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Sample records for curl philippines virus

  1. Transmission of Sweet Potato Leaf Curl Virus by Bemisia tabaci

    Science.gov (United States)

    Sweetpotato, Ipomoea batatas (L.) Lam. (Solanales: Convolvulaceae), is an important world food crop, and Asia is the focal production region. Because it is vegetatively propagated, sweetpotato is especially prone to accumulate infections by several viruses. Sweet potato leaf curl virus (SPLCV) (ss...

  2. The complete nucleotide sequence of pelargonium leaf curl virus.

    Science.gov (United States)

    McGavin, Wendy J; MacFarlane, Stuart A

    2016-05-01

    Investigation of a tombusvirus isolated from tulip plants in Scotland revealed that it was pelargonium leaf curl virus (PLCV) rather than the originally suggested tomato bushy stunt virus. The complete sequence of the PLCV genome was determined for the first time, revealing it to be 4789 nucleotides in size and to have an organization similar to that of the other, previously described tombusviruses. Primers derived from the sequence were used to construct a full-length infectious clone of PLCV that recapitulates the disease symptoms of leaf curling in systemically infected pelargonium plants.

  3. Pepper leaf curl Lahore virus requires the DNA B component of Tomato leaf curl New Delhi virus to cause leaf curl symptoms

    Directory of Open Access Journals (Sweden)

    Briddon Rob W

    2010-12-01

    Full Text Available Abstract Background Begomoviruses are whitefly-transmitted geminiviruses with genomes that consist of either two components (known as DNA A and DNA B or a single component (homologous to the DNA A component of bipartite begomoviruses. Monopartite begomoviruses are often associated with a symptom-modulating DNA satellite (collectively known as betasatellites. Both bipartite and monopartite begomoviruses with associated satellites have previously been identified in chillies showing leaf curl symptoms in Pakistan. Results A chilli plant (Capsicum annum with chilli leaf curl disease symptoms was found to contain a begomovirus, a betasatellite and the DNA B component of Tomato leaf curl New Delhi virus (ToLCNDV. The begomovirus consisted of 2747 nucleotides and had the highest sequence identity (99% with Pepper leaf curl Lahore virus (PepLCLV-[PK: Lah:04], acc. no. AM404179. Agrobacterium-mediated inoculation of the clone to Nicotiana benthamiana, induced very mild symptoms and low levels of viral DNA, detected in systemically infected leaves by PCR. No symptoms were induced in Nicotiana tabacum or chillies either in the presence or absence of a betasatellite. However, inoculation of PepLCLV with the DNA B component of ToLCNDV induced leaf curl symptoms in N. benthamiana, N. tabacum and chillies and viral DNA accumulated to higher levels in comparison to plants infected with just PepLCLV. Conclusions Based on our previous efforts aimed at understanding of diversity of begomoviruses associated with chillies, we propose that PepLCLV was recently mobilized into chillies upon its interaction with DNA B of ToLCNDV. Interestingly, the putative rep-binding iterons found on PepLCLV (GGGGAC differ at two base positions from those of ToLCNDV (GGTGTC. This is the first experimental demonstration of the infectivity for a bipartite begomovirus causing chilli leaf curl disease in chillies from Pakistan and suggests that component capture is contributing to the

  4. Molecular Characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite Naturally Infecting Pumpkin (Cucurbita moschata) in India.

    Science.gov (United States)

    Namrata, Jaiswal; Saritha, R K; Datta, D; Singh, M; Dubey, R S; Rai, A B; Rai, M

    2010-10-01

    Pumpkin cultivation in India is affected by severe incidence of a yellow vein mosaic disease. Tomato leaf curl New Delhi virus and Squash leaf curl China virus are known to be associated with this disease in India. We were able to identify a third begomovirus-Tomato leaf curl Palampur virus (ToLCPMV), from pumpkin showing typical symptoms of the disease at Varanasi based on the sequence of complete DNA-A genome of the virus. The complete DNA-A sequence of the virus shared more than 99% sequence identity with other ToLCPMV isolates available in the GenBank and clustered with them in the phylogenetic analysis. This betasatellite amplified from the same infected sample has been identified as Pepper leaf curl betasatellite (PepLCB) which also infects chilli in India. There was 92% sequence identity between the two isolates. This is the first report of natural infection of ToLCPMV on pumpkin and association of PepLCB with yellow vein mosaic disease of pumpkin in India.

  5. Evaluating Weeds as Hosts of Tomato yellow leaf curl virus.

    Science.gov (United States)

    Smith, Hugh A; Seijo, Teresa E; Vallad, Gary E; Peres, Natalia A; Druffel, Keri L

    2015-08-01

    Bemisia tabaci (Gennadius) biotype B transmits Tomato yellow leaf curl virus (TYLCV), which affects tomato production globally. Prompt destruction of virus reservoirs is a key component of virus management. Identification of weed hosts of TYLCV will be useful for reducing such reservoirs. The status of weeds as alternate hosts of TYLCV in Florida remains unclear. In greenhouse studies, B. tabaci adults from a colony reared on TYLCV-infected tomato were established in cages containing one of four weeds common to horticultural fields in central and south Florida. Cages containing tomato and cotton were also infested with viruliferous whiteflies as a positive control and negative control, respectively. Whitefly adults and plant tissue were tested periodically over 10 wk for the presence of TYLCV using PCR. After 10 wk, virus-susceptible tomato plants were placed in each cage to determine if whiteflies descended from the original adults were still infective. Results indicate that Bidens alba, Emilia fosbergii, and Raphanus raphanistrum are not hosts of TYLCV, and that Amaranthus retroflexus is a host. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  6. Sweet Potato Leaf Curl Virus: Virus Reservoir in Species of Wild Morning Glory

    Science.gov (United States)

    Recent increases in populations of the Sweetpotato leaf curl virus (SPLCV) vector, the sweetpotato whitefly, Bemisia tabaci (Gennadius), led to a dramatic increase in the disease in sweetpotato (Ipomoea batatas). Knowledge of crop or weed species that occur in sweetpotato growing areas and can serv...

  7. Senna leaf curl virus: a novel begomovirus identified in Senna occidentalis.

    Science.gov (United States)

    Kumar, Jitesh; Alok, Anshu; Kumar, Jitendra; Tuli, Rakesh

    2016-09-01

    Begomoviruses are whitefly-transmitted, single-stranded DNA viruses that infect a variety of cultivated (crop) and non-cultivated (weed) plants. The present study identified a novel begomovirus and satellites (alpha- and betasatellite) in Senna occidentalis (syn. Cassia occidentalis) showing leaf curl symptoms. The begomovirus shared a maximum sequence identity of 88.6 % with french bean leaf curl virus (JQ866297), whereas the alphasatellite and the betasatellite shared identities of 98 % and 90 % with ageratum yellow vein India alphasatellite (LK054802) and papaya leaf curl betasatellite (HM143906), respectively. No other begomovirus or satellites were detected in the suspected plants. We propose to name the virus "senna leaf curl virus" (SenLCuV).

  8. Novel Synthetic Promoters from the Cestrum Yellow Leaf Curling Virus.

    Science.gov (United States)

    Sahoo, Dipak Kumar; Sarkar, Shayan; Maiti, Indu B; Dey, Nrisingha

    2016-01-01

    Constitutive promoters direct gene expression uniformly in most tissues and cells at all stages of plant growth and development; they confer steady levels of transgene expression in plant cells and hence their demand is high in plant biology. The gene silencing due to promoter homology can be avoided by either using diverse promoters isolated from different plant and viral genomes or by designing synthetic promoters. The aim of this chapter was to describe the basic protocols needed to develop and analyze novel, synthetic, nearly constitutive promoters from Cestrum yellow leaf curling virus (CmYLCV) through promoter/leader deletion and activating cis-sequence analysis. We also describe the methods to evaluate the strength of the promoters efficiently in various transient expression systems like agroinfiltration assay, gene-gun method, and assay in tobacco protoplasts. Besides, the detailed methods for developing transgenic plants (tobacco and Arabidopsis) for evaluation of the promoter using the GUS reporter gene are also described. The detailed procedure for electrophoretic mobility shift assay (EMSA) coupled with super-shift EMSA analysis are also described for showing the binding of tobacco transcription factor, TGA1a to cis-elements in the CmYLCV distal promoter region.

  9. First Report of Tomato yellow leaf curl virus in Tomato in the Netherlands

    NARCIS (Netherlands)

    Botermans, M.; Verhoeven, J.Th.J.; Jansen, C.C.C.; Roenhorst, J.W.; Stijger, C.C.M.M.; Pham, K.T.K.

    2009-01-01

    Tomato yellow leaf curl virus (TYLCV) is an economically important virus with tomato (Solanum lycopersicum L.) as its main host. The virus is widely distributed in subtropical areas and is transmitted by the tobacco whitefly (Bemisia tabaci) in a persistent manner. TYLCV has a quarantine status

  10. First Report of Tomato yellow leaf curl virus in Tomato in the Netherlands

    NARCIS (Netherlands)

    Botermans, M.; Verhoeven, J.Th.J.; Jansen, C.C.C.; Roenhorst, J.W.; Stijger, C.C.M.M.; Pham, K.T.K.

    2009-01-01

    Tomato yellow leaf curl virus (TYLCV) is an economically important virus with tomato (Solanum lycopersicum L.) as its main host. The virus is widely distributed in subtropical areas and is transmitted by the tobacco whitefly (Bemisia tabaci) in a persistent manner. TYLCV has a quarantine status (IIA

  11. BIO-EFFICACY OF NEWER INSECTICIDES AGAINST TOMATO LEAF CURL VIRUS DISEASE AND ITS VECTOR WHITEFLY (BEMISIA TABACI) IN TOMATO

    National Research Council Canada - National Science Library

    Govindappa, M.R; Bhemanna, M; Arunkumar Hosmani; V.N. Ghante

    2013-01-01

    .... Present investigations on Invitro efficacy of new insecticide molecules on whitefly mortality and leaf curl virus transmission revealed that adult mortality varies with the length of incubation...

  12. Phylogenetic analysis of Melon chlorotic leaf curl virus from Guatemala: Another emergent species in the Squash leaf curl virus clade

    KAUST Repository

    Brown, J.K.

    2011-06-01

    The genome of a new bipartite begomovirus Melon chlorotic leaf curl virus from Guatemala (MCLCuV-GT) was cloned and the genome sequence was determined. The virus causes distinct symptoms on melons that were not previously observed in melon crops in Guatemala or elsewhere. Phylogenetic analysis of MCLCuV-GT and begomoviruses infecting cucurbits and other host plant species indicated that its closest relative was MCLCuV from Costa Rica (MCLCuV-CR). The DNA-A components of two isolates shared 88.8% nucleotide identity, making them strains of the same species. Further, both MCLCuV-GT and MCLCuV-CR grouped with other Western Hemisphere cucurbit-infecting species in the SLCV-clade making them the most southerly cucurbit-infecting members of the clade to date. Although the common region of the cognate components of MCLCuV-GT and MCLCuV-CR, shared similar to 96.3% nucleotide identity. While DNA-A and DNA-B components of MCLCuV-GT were less than 86% nucleotide identity with the respective DNAA and DNA-B common regions of MCLCuV-CR. The late viral genes of the two strains shared the least nt identity (<88%) while their early genes shared the highest nt identity (>90%). The collective evidence suggests that these two strains of MCLCuV are evolutionarily divergent owing in part to recombination, but also due to the accumulation of a substantial number of mutations. In addition they are differentially host-adapted, as has been documented for other cucurbit-infecting, bean-adapted, species in the SLCV clade. (C) 2011 Elsevier B.V. All rights reserved.

  13. Chinese squash leaf curl virus: a new whitefly-transmitted geminivirus

    Institute of Scientific and Technical Information of China (English)

    洪益国; 王小凤; 田波; 蔡健和

    1995-01-01

    Coat protein (CP) gene of the Chinese squash !eaf curl virus (SqLCV-C) was amplified through PC’R, cloned and completely sequenced. Based on the comparisons at the levels of both CP gene nucleotide and CP -deduced amino acid sequences with other geminiviruses, SqLCV-C is confirmed to be distinct from the American squash leaf curl virus (SqLCV-E). It is a new geminivirus transmitted by whitefly Bemisia tabaci, which infects dicotyledonous plants and is more closely related to the Indian cassava mosaic virus (ICMV).

  14. Management of sweet potato leaf curl virus in sweetpotatoes using insecticides

    Science.gov (United States)

    Sweetpotato leaf curl virus (SPLCV), which is transmitted by the sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), can severely affect yields of commercial sweetpotatoes, Ipomoea batatas (L.) Lam. (Convolvulaceae). This virus occurs every year at the U.S. Vegetable Laborato...

  15. Whitefly transmission of Sweet potato leaf curl virus in sweetpotato germplasm

    Science.gov (United States)

    Sweetpotato, Ipomoea batatas (L.) Lam., is among an extensive number of plant species attacked by Bemisia tabaci (Gennadius). Because this important world food crop is vegetatively propagated, it can conveniently accumulate infections by several viruses. Sweet potato leaf curl virus (SPLCV) (ssDNA...

  16. Molecular Characterization of Chikungunya Virus, Philippines, 2011-2013.

    Science.gov (United States)

    Sy, Ava Kristy; Saito-Obata, Mariko; Medado, Inez Andrea; Tohma, Kentaro; Dapat, Clyde; Segubre-Mercado, Edelwisa; Tandoc, Amado; Lupisan, Socorro; Oshitani, Hitoshi

    2016-05-01

    During 2011-2013, a nationwide outbreak of chikungunya virus infection occurred in the Philippines. The Asian genotype was identified as the predominant genotype; sporadic cases of the East/Central/South African genotype were detected in Mindanao. Further monitoring is needed to define the transmission pattern of this virus in the Philippines.

  17. Chinese tomato yelIow Ieaf curl virus- a new species of geminivirus.

    Science.gov (United States)

    Liu, Y; Cai, J; Li, D; Qin, B; Tian, B

    1998-08-01

    Chine tomato yellow leaf curl virus (TYLCV-CHI) and other geminiviruses were analysed with 20 monoclonaI antibodies. It was shown that TYLCV-CHI is serclogicaIly close to Chinese tabacco Ieaf curl virus (TbLCV-CHI). The fragment of TYLCV-CHI DNA including the common region (CR), N-terminal of coat protein gene and AV1 gene was amplified by PCR and cloned, and its DNA sequence was determined. These raults showed that TYLCV-CHI is different from other known geminiviruses in the world, and is a new whitefly-transmitted gerninivirus.

  18. Phylogenetic lineage of Tobacco leaf curl virus in Korea and estimation of recombination events implicated in their sequence variation.

    Science.gov (United States)

    Park, Jungan; Lee, Hyejung; Kim, Mi-Kyung; Kwak, Hae-Ryun; Auh, Chung-Kyoon; Lee, Kyeong-Yeoll; Kim, Sunghan; Choi, Hong-Soo; Lee, Sukchan

    2011-08-01

    New strains of Tobacco leaf curl virus (TbLCV) were isolated from tomato plants in four different local communities of Korea, and hence were designated TbLCV-Kr. Phylogenetic analysis of the sequences of the whole genome and of individual ORFs of these viruses indicated that they are closely related to the Tobacco leaf curl Japan virus (TbLCJV) cluster, which includes Honeysuckle yellow vein virus (HYVV), Honeysuckle yellow vein mosaic virus (HYVMV), and TbLCJV isolates. Four putative recombination events were recognized within these virus sequences, suggesting that the sequence variations observed in these viruses may be attributable to intraspecific and interspecific recombination events involving some TbLCV-Kr isolates, Papaya leaf curl virus (PaLCV), and a local isolate of Tomato yellow leaf curl virus (TYLCV).

  19. Relationship between Tomato yellow leaf curl viruses and the whitefly vector.

    Science.gov (United States)

    Ssekyewa, Charles; Van Damme, Patrick L J; Hofte, Monica

    2007-01-01

    Tomato Yellow Leaf Curl Virus (TYLCV) and Tomato Leaf Curl Virus (ToLCV) are the currently known begomoviruses in Uganda. The relationship with their whiteflies (Bemisia tabaci) vector and its management were not known in Uganda. A direct relationship was expected between these begomoviruses and whiteflies at Buwama in Mpigi district of Uganda. Farmer practices were expected to have limited efficacy. To investigate this, a completely randomised block design was used for all trials with six treatments replicated three times. Treatments evaluated were farmer whitefly pest management practices. These included both chemical pesticide and non-pesticide applications. Data on whitefly population and tomato yellow leaf curl virus disease incidence was recorded weekly, and analysed using SAS and SPSS statistical programmes for ANOVA, and correlations. Ranked means, coefficients of variation and standard errors were noted. Virus-vector relationship field studies established that virus occurrence varied in space and time, and with management practices, crop development stage, and weather conditions. A negative relationship (R = -0.14, p 0.04) was established between number of plants infected with TYLCV (sensu lato) and percentage marketable tomato yield. Tomato maturity was inversely propotional to whitefly infestation (R = -0.5, p 0.0001). Uprooting and application of dimethoate was most effective of the six treatments. Tomato leaf curl virus diseases and whitefly management options were established in Uganda.

  20. Transcript mapping of Cotton leaf curl Burewala virus and its cognate betasatellite, Cotton leaf curl Multan betasatellite

    Directory of Open Access Journals (Sweden)

    Akbar Fazal

    2012-10-01

    Full Text Available Abstract Background Whitefly-transmitted geminiviruses (family Geminiviridae, genus Begomovirus are major limiting factors for the production of numerous dicotyledonous crops throughout the warmer regions of the world. In the Old World a small number of begomoviruses have genomes consisting of two components whereas the majority have single-component genomes. Most of the monopartite begomoviruses associate with satellite DNA molecules, the most important of which are the betasatellites. Cotton leaf curl disease (CLCuD is one of the major problems for cotton production on the Indian sub-continent. Across Pakistan, CLCuD is currently associated with a single begomovirus (Cotton leaf curl Burewala virus [CLCuBuV] and the cotton-specific betasatellite Cotton leaf curl Multan betasatellite (CLCuMuB, both of which have recombinant origins. Surprisingly, CLCuBuV lacks C2, one of the genes present in all previously characterized begomoviruses. Virus-specific transcripts have only been mapped for few begomoviruses, including one monopartite begomovirus that does not associate with betasatellites. Similarly, the transcripts of only two betasatellites have been mapped so far. The study described has investigated whether the recombination/mutation events involved in the evolution of CLCuBuV and its associated CLCuMuB have affected their transcription strategies. Results The major transcripts of CLCuBuV and its associated betasatellite (CLCuMuB from infected Nicotiana benthamiana plants have been determined. Two complementary-sense transcripts of ~1.7 and ~0.7 kb were identified for CLCuBuV. The ~1.7 kb transcript appears similar in position and size to that of several begomoviruses and likely directs the translation of C1 and C4 proteins. Both complementary-sense transcripts can potentially direct the translation of C2 and C3 proteins. A single virion-sense transcript of ~1 kb, suitable for translation of the V1 and V2 genes was identified. A predominant

  1. There is the second virus that causes tobacco leaf curl disease (not TbLCV-CHI) in the field

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Sequence analysis of virus isolation DNA of tobacco leaf curl disease shows that there is the second geminivirus (not Chinese Tobacco Leaf Curl Virus, TbLCV-CHI) that causes tobacco leaf curl disease in the field in the Guangxi Zhuang Autonomous Region, China. This virus DNA-A contains 2 734 nt. Large intergenic region (LIR) contains 269 nt, the virus sense strand contains 2 open reading frames (ORFs): AV1 (115 aa) and AV2 (coat protein gene, CP, 256 aa), and the complementary sense strand contains 4 ORFs: AC1 (replicase gene, 361 aa), AC2 (transactivator, 134 aa), AC3 (134 aa) and AC4 (97 aa). The virus belongs to one kind of subgroup Ⅲ gemini- viruses from old world, and could be the Chinese tomato yellow leaf curl virus (TYLCV-CHI).

  2. Is the begomovirus, sweet potato leaf curl virus, really seed transmitted in sweetpotato?

    Science.gov (United States)

    Sweetpotato is one of the major root crops in the world and is also widely grown in the southern United States. Sweet potato leaf curl virus (SPLCV) is a begomovirus posing a serious threat to sweetpotato production worldwide and is primarily transmitted by whitefly (Bemisia tabaci) or through veget...

  3. Discovery and demonstration of small circular DNA molecules derived from Chinese tomato yellow leaf curl virus

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Tomato yellow leaf curl viruses belong to Begomoviruses of geminiviruses.In this work, we first found and demonstrated that the small circular DNA molecules were derived from Chinese tomato yellow leaf curl viruses (TYLCV-CHI).These small circular DNA molecules are about 1.3 kb, which are half the full-length of TYLCV-CHI DNA A.It was shown by sequence determination and analysis that there was unknown-origin sequence insertion in the middle of the small molecules.These sequences of unknown-origin were neither homologous to DNA A nor to DNA B, and were formed by recombination of virus DNA and plant DNA.Although various defective molecules contained different unknown-origin sequence insertion, all the molecules contained the intergenic region and part of the AC1(Rep) gene.But they did not contain full ORF.

  4. Molecular characterization and pathogenicity of tomato yellow leaf curl virus in China.

    Science.gov (United States)

    Zhang, Hui; Gong, Huanran; Zhou, Xueping

    2009-10-01

    Several tomato production regions in China were surveyed for tomato yellow leaf curl disease (TYLCD), and 31 tomato leaf samples showing TYLCD-like symptoms were collected. The partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (TYLCV) was detected in Shanghai, Zhejiang, Jiangsu Shandong and Hebei provinces of China. The TYLCV isolates found in China share high sequence identity ([98%) and have more than 97% sequence identity with TYLCVIL[ IL:Reo] (X15656). Phylogenetic relationship analysis reveals that although with little genetic variability, they can form two groups and all the TYLCV isolates in China belong to the group I. An infectious clone of TYLCV-[CN:SH2] (AM282874) was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum, Petunia hybrida, Cucumis sativus, Gossypium hirsutum, S. melongena, and Capsicum annuum. TYLCV-[CN:SH2] can induce severe leaf curling and stunting symptoms in these plants except C. sativus, G. hirsutum, S. melongena and C. annuum.We verified that TYLCV can trans-replicate tomato yellow leaf curl China virus DNA-b in N. benthamiana and S. lycopersicum and induced more severe symptoms with distortion and yellow vein.

  5. Tomato yellow leaf curl viruses: ménage à trois between the virus complex, the plant and the whitefly vector.

    Science.gov (United States)

    Díaz-Pendón, Juan Antonio; Cañizares, M Carmen; Moriones, Enrique; Bejarano, Eduardo R; Czosnek, Henryk; Navas-Castillo, Jesús

    2010-07-01

    Tomato yellow leaf curl disease (TYLCD) is one of the most devastating viral diseases affecting tomato crops in tropical, subtropical and temperate regions of the world. Here, we focus on the interactions through recombination between the different begomovirus species causing TYLCD, provide an overview of the interactions with the cellular genes involved in viral replication, and highlight recent progress on the relationships between these viruses and their vector, the whitefly Bemisia tabaci. The tomato yellow leaf curl virus-like viruses (TYLCVs) are a complex of begomoviruses (family Geminiviridae, genus Begomovirus) including 10 accepted species: Tomato yellow leaf curl Axarquia virus (TYLCAxV), Tomato yellow leaf curl China virus (TYLCCNV), Tomato yellow leaf curl Guangdong virus (TYLCGuV), Tomato yellow leaf curl Indonesia virus (TYLCIDV), Tomato yellow leaf curl Kanchanaburi virus (TYLVKaV), Tomato yellow leaf curl Malaga virus (TYLCMalV), Tomato yellow leaf curl Mali virus (TYLCMLV), Tomato yellow leaf curl Sardinia virus (TYLCSV), Tomato yellow leaf curl Thailand virus (TYLCTHV), Tomato yellow leaf curl Vietnam virus (TYLCVNV) and Tomato yellow leaf curl virus(TYLCV). We follow the species demarcation criteria of the International Committee on Taxonomy of Viruses (ICTV), the most important of which is an 89% nucleotide identity threshold between full-length DNA-A component nucleotide sequences for begomovirus species. Strains of a species are defined by a 93% nucleotide identity threshold. The primary host of TYLCVs is tomato (Solanum lycopersicum), but they can also naturally infect other crops [common bean (Phaseolus vulgaris), sweet pepper (Capsicum annuum), chilli pepper (C. chinense) and tobacco (Nicotiana tabacum)], a number of ornamentals [petunia (Petuniaxhybrida) and lisianthus (Eustoma grandiflora)], as well as common weeds (Solanum nigrum and Datura stramonium). TYLCVs also infect the experimental host Nicotiana benthamiana. Infected tomato

  6. Tomato Leaf Curl New Delhi Virus: An Emerging Virus Complex Threatening Vegetable and Fiber Crops

    Directory of Open Access Journals (Sweden)

    Enrique Moriones

    2017-09-01

    Full Text Available The tomato leaf curl New Delhi virus (ToLCNDV (genus Begomovirus, family Geminiviridae represents an important constraint to tomato production, as it causes the most predominant and economically important disease affecting tomato in the Indian sub-continent. However, in recent years, ToLCNDV has been fast extending its host range and spreading to new geographical regions, including the Middle East and the western Mediterranean Basin. Extensive research on the genome structure, protein functions, molecular biology, and plant–virus interactions of ToLCNDV has been conducted in the last decade. Special emphasis has been given to gene silencing suppression ability in order to counteract host plant defense responses. The importance of the interaction with DNA alphasatellites and betasatellites in the biology of the virus has been demonstrated. ToLCNDV genetic variability has been analyzed, providing new insights into the taxonomy, host adaptation, and evolution of this virus. Recombination and pseudorecombination have been shown as motors of diversification and adaptive evolution. Important progress has also been made in control strategies to reduce disease damage. This review highlights these various achievements in the context of the previous knowledge of begomoviruses and their interactions with plants.

  7. Tomato Leaf Curl New Delhi Virus: An Emerging Virus Complex Threatening Vegetable and Fiber Crops.

    Science.gov (United States)

    Moriones, Enrique; Praveen, Shelly; Chakraborty, Supriya

    2017-09-21

    The tomato leaf curl New Delhi virus (ToLCNDV) (genus Begomovirus, family Geminiviridae) represents an important constraint to tomato production, as it causes the most predominant and economically important disease affecting tomato in the Indian sub-continent. However, in recent years, ToLCNDV has been fast extending its host range and spreading to new geographical regions, including the Middle East and the western Mediterranean Basin. Extensive research on the genome structure, protein functions, molecular biology, and plant-virus interactions of ToLCNDV has been conducted in the last decade. Special emphasis has been given to gene silencing suppression ability in order to counteract host plant defense responses. The importance of the interaction with DNA alphasatellites and betasatellites in the biology of the virus has been demonstrated. ToLCNDV genetic variability has been analyzed, providing new insights into the taxonomy, host adaptation, and evolution of this virus. Recombination and pseudorecombination have been shown as motors of diversification and adaptive evolution. Important progress has also been made in control strategies to reduce disease damage. This review highlights these various achievements in the context of the previous knowledge of begomoviruses and their interactions with plants.

  8. Coat protein promoter from cotton leaf curl virus is not a tissue-specifically expressed promoter

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Geminivirus is a kind of single-stranded DNA virus. Experimental results from tomato golden mosaic virus (TGMV) showed that expression pattern of coat protein gene (cp) promoter was phloem specifically expressed. In this note, the studies on cp promoter of cotton leaf curl virus (CLCuV) which is found and identified recently suggest that the promoter is not phloem specifically expressed. The expressing activity of gus gene driven by the promoter exists not only in phloem but also in mesophyll tissues and root tip meristem. Transient expression suggests that cp promoter transactivated by AC2 shows expressing activity in mesophyll and vascular tissue of leaf vein.

  9. Serological and molecular identification of Tomato yellow leaf curl virus in Khuzestan province of Iran

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    Shahrokh MALEKZADEH

    2011-09-01

    Full Text Available A survey was conducted from 2006 to 2007 to identify the causal agent of leaf curling of tomato in eight major tomato-growing areas of Khuzestan province in southwest of Iran. Tomato leaf samples showing leaf curling, yellowing, and stunting were collected and screened for the presence of Tomato yellow leaf curl virus (TYLCV by TAS-ELISA. Further confi rmation was completed using graft transmission onto healthy tomato plants and PCR. Results confi rmed that TYLCV is a causal agent of tomato yellow leaf curl disease (TYLCD and is widely distributed in all the major tomato growing areas in southwest of Iran. The nucleotide sequences of the coat protein (CP gene of four isolates (Dezfoul, Shoush, Behbahan, and Ramhormoz were determined and deposited in GenBank (EF199814-7. Phylogenetic analysis of the CP gene further showed that all four Iranian isolates have very close relationship and formed a Compact cluster together with previously sequenced Iranian TYLCV isolates.

  10. Phylogeographic analysis of rabies viruses in the Philippines.

    Science.gov (United States)

    Tohma, Kentaro; Saito, Mariko; Kamigaki, Taro; Tuason, Laarni T; Demetria, Catalino S; Orbina, Jun Ryan C; Manalo, Daria L; Miranda, Mary E; Noguchi, Akira; Inoue, Satoshi; Suzuki, Akira; Quiambao, Beatriz P; Oshitani, Hitoshi

    2014-04-01

    Rabies still remains a public health threat in the Philippines. A significant number of human rabies cases, about 200-300 cases annually, have been reported, and the country needs an effective strategy for rabies control. To develop an effective control strategy, it is important to understand the transmission patterns of the rabies viruses. We conducted phylogenetic analyses by considering the temporal and spatial evolution of rabies viruses to reveal the transmission dynamics in the Philippines. After evaluating the molecular clock and phylogeographic analysis, we estimated that the Philippine strains were introduced from China around the beginning of 20th century. Upon this introduction, the rabies viruses evolved within the Philippines to form three major clades, and there was no indication of introduction of other rabies viruses from any other country. However, within the Philippines, island-to-island migrations were observed. Since then, the rabies viruses have diffused and only evolved within each island group. The evolutionary pattern of these viruses was strongly shaped by geographical boundaries. The association index statistics demonstrated a strong spatial structure within the island group, indicating that the seas were a significant geographical barrier for viral dispersal. Strong spatial structure was also observed even at a regional level, and most of the viral migrations (79.7% of the total median number) in Luzon were observed between neighboring regions. Rabies viruses were genetically clustered at a regional level, and this strong spatial structure suggests a geographical clustering of transmission chains and the potential effectiveness of rabies control that targets geographical clustering. Dog vaccination campaigns have been conducted independently by local governments in the Philippines, but it could be more effective to implement a coordinated vaccination campaign among neighboring areas to eliminate geographically-clustered rabies

  11. Replication of Tomato Yellow Leaf Curl Virus in Its Whitefly Vector, Bemisia tabaci.

    Science.gov (United States)

    Pakkianathan, Britto Cathrin; Kontsedalov, Svetlana; Lebedev, Galina; Mahadav, Assaf; Zeidan, Muhammad; Czosnek, Henryk; Ghanim, Murad

    2015-10-01

    Tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted exclusively by the whitefly Bemisia tabaci in a persistent, circulative manner. Replication of TYLCV in its vector remains controversial, and thus far, the virus has been considered to be nonpropagative. Following 8 h of acquisition on TYLCV-infected tomato plants or purified virions and then transfer to non-TYLCV-host cotton plants, the amounts of virus inside whitefly adults significantly increased (>2-fold) during the first few days and then continuously decreased, as measured by the amounts of genes on both virus DNA strands. Reported alterations in insect immune and defense responses upon virus retention led us to hypothesize a role for the immune response in suppressing virus replication. After virus acquisition, stress conditions were imposed on whiteflies, and the levels of three viral gene sequences were measured over time. When whiteflies were exposed to TYLCV and treatment with two different pesticides, the virus levels continuously increased. Upon exposure to heat stress, the virus levels gradually decreased, without any initial accumulation. Switching of whiteflies between pesticide, heat stress, and control treatments caused fluctuating increases and decreases in virus levels. Fluorescence in situ hybridization analysis confirmed these results and showed virus signals inside midgut epithelial cell nuclei. Combining the pesticide and heat treatments with virus acquisition had significant effects on fecundity. Altogether, our results demonstrate for the first time that a single-stranded DNA plant virus can replicate in its hemipteran vector. Plant viruses in agricultural crops are of great concern worldwide. Many of them are transmitted from infected to healthy plants by insects. Persistently transmitted viruses often have a complex association with their vectors; however, most are believed not to replicate within these vectors. Such replication is important, as it contributes to the

  12. Cotton Leaf Curl Virus: lonic Status of Leaves and Symptom Development

    Institute of Scientific and Technical Information of China (English)

    Naeem Iqbal; Muhammad Yasin Ashraf; Farrukh Javed; Muhammad Ashraf; Sohail Hameed

    2006-01-01

    In the present study, the relationship between the nutritional status of leaves and the development of symptoms of cotton leaf curl virus (CLCuV) in two cotton (Gossypium hirsutum L.) cultivars (i.e. CIM-240 and S-12) was investigated. The incidence of disease attack was found to be 100% in the S-12 cultivar and 16% in the CIM-240 cultivar. Geminivirus particles in infected leaves were confirmed by transmission electron microscope examination of highly specific geminivirus coat protein antisera-treated cell sap. The CLCuV impaired the accumulation of different nutrients in both cultivars. A marked decrease in the accumulation of Ca2+ and K+ was observed in infected leaves. However, the disease had no effect on leaf concentrations of Na+, N, and P. It was observed that the curling of leaf margins in CLCuV-infected plants was associated with the leaf Ca2+ content; leaf curling was severe in plants with a significant reduction in Ca2+ content.Moreover, leaf K+ content was found to be associated with resistance/susceptibility to CLCuV infection.

  13. Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato

    NARCIS (Netherlands)

    Yang, X.; Caro Rios, C.M.; Hutton, S.F.; Scott, J.W.; Guo, Y.; Wang, Xiaoxuan; Rashid, H.; Szinay, D.; Jong, de J.H.S.G.M.; Visser, R.G.F.; Bai, Y.; Du, Y.

    2014-01-01

    Resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (TYLCV), have been introgressed to cultivated tomato (Solanumlycopersicum) fromwild tomato accessions. A major gene, Ty-2 from S. habrochaites f. glabratum accession ‘‘B6013,’’ that

  14. Natural association of two different betasatellites with Sweet potato leaf curl virus in wild morning glory (Ipomoea purpurea) in India.

    Science.gov (United States)

    Swapna Geetanjali, A; Shilpi, S; Mandal, Bikash

    2013-08-01

    Wild morning glory (Ipomoea purpurea) was observed to be affected by leaf curl and yellow vein diseases during summer-rainy season of 2009 in New Delhi, India. The virus was experimentally transmitted through whitefly, Bemisia tabaci to I. purpurea that reproduced the two distinct symptoms. Sequence analysis of multiple full-length clones obtained through rolling circle amplification from the leaf curl and yellow vein samples showed 91.8-95.3% sequence identity with Sweet potato leaf curl virus (SPLCV) and the isolates were phylogenetically distinct from those reported from Brazil, China, Japan and USA. Interestingly, two different betasatellites, croton yellow vein mosaic betasatellite and papaya leaf curl betasatellite were found with SPLCV in leaf curl and yellow vein diseases of I. purpurea, respectively. This study is the first report of occurrence of SPLCV in wild morning glory in India. SPLCV was known to infect other species of morning glory; our study revealed that I. purpurea, a new species of morning glory was a natural host of SPLCV. To date, betasatellite associated with SPLCV in Ipomoea spp. is not known. Our study provides evidence of natural association of two different betasatellites with SPLCV in leaf curl and yellow vein diseases of I. purpurea.

  15. Seed Transmission of Tomato yellow leaf curl virus in White Soybean (Glycine max).

    Science.gov (United States)

    Kil, Eui-Joon; Park, Jungho; Choi, Hong-Soo; Kim, Chang-Seok; Lee, Sukchan

    2017-08-01

    Tomato yellow leaf curl virus (TYLCV) infection of the common bean (Phaseolus vulgaris) has been reported, but soybean (Glycine max) has not previously been identified as a TYLCV host. Five cultivars of white soybean were agro-inoculated using an infectious TYLCV clone. At 30 days post-inoculation, they showed infection rates of 25% to 100%. Typical TYLCV symptoms were not observed in any inoculated plants. To examine whether TYLCV was transmitted in soybean seeds, DNA was isolated from bundles of five randomly selected seeds from TYLCV-inoculated soybean plants and amplified with a TYLCV-specific primer set. With the exception of one bundle, all bundles of seeds were verified to be TYLCV-infected. Virus dissemination was also confirmed in three of the 14 bunches. Viral replication was also identified in seeds and seedlings. This is the first report demonstrating that soybean is a TYLCV host, and that TYLCV is a seed-transmissible virus in white soybean.

  16. Detection and molecular characterization of tomato yellow leaf curl virus naturally infecting Lycopersicon esculentum in Egypt.

    Science.gov (United States)

    Rabie, M; Ratti, C; Abdel Aleem, E; Fattouh, F

    2017-01-01

    Tomato yellow leaf curl virus (TYLCV) infections of tomato crops in Egypt were widely spread in 2014. Infected symptomatic tomato plants from different governorates were sampled. TYLCV strains Israel and Mild (TYLCV-IL, TYLCV-Mild) were identified by multiplex and real-time PCR. In addition, nucleotide sequence analysis of the V1 and V2 protein genes, revealed ten TYLCV Egyptian isolates (TYLCV from TY1 to 10). Phylogenetic analysis showed their high degree of relatedness with TYLCV-IL Jordan isolate (98%). Here we have showed the complete nucleotide sequence of the TYLCV Egyptian isolate TY10, sampled from El Beheira. A high degree of similarity to other previously reported Egyptian isolates and isolates from Jordan and Japan reflect the importance of phylogenetic analysis in monitoring virus genetic diversity and possibilities for divergence of more virulent strains or genotypes.

  17. A Novel Strain of Tomato Leaf Curl New Delhi Virus Has Spread to the Mediterranean Basin

    Directory of Open Access Journals (Sweden)

    Isabel M. Fortes

    2016-11-01

    Full Text Available Tomato leaf curl New Delhi virus (ToLCNDV is a whitefly-transmitted bipartite begomovirus (genus Begomovirus, family Geminiviridae that causes damage to multiple cultivated plant species mainly belonging to the Solanaceae and Cucurbitaceae families. ToLCNDV was limited to Asian countries until 2012, when it was first reported in Spain, causing severe epidemics in cucurbit crops. Here, we show that a genetically-uniform ToLCNDV population is present in Spain, compatible with a recent introduction. Analyses of ToLCNDV isolates reported from other parts of the world indicated that this virus has a highly heterogeneous population genetically with no evident geographical, plant host or year-based phylogenetic groups observed. Isolates emerging in Spain belong to a strain that seems to have evolved by recombination. Isolates of this strain seem adapted to infecting cucurbits, but poorly infect tomatoes.

  18. Natural Occurrence of Tomato leaf curl New Delhi virus in Iranian Cucurbit Crops

    Directory of Open Access Journals (Sweden)

    Sara Yazdani-Khameneh

    2016-06-01

    Full Text Available The main areas for field-grown vegetable production in Iran were surveyed during the years of 2012–2014 to determine the occurrence of begomoviruses infecting these crops. A total of 787 leaf samples were collected from vegetables and some other host plants showing virus-like symptoms and tested by an enzyme-linked immunosorbent assay (ELISA using polyclonal antibodies produced against Tomato yellow leaf curl virus (TYLCV. According to the ELISA results, 81 samples (10.3% positively reacted with the virus antibodies. Begomovirus infections were confirmed by polymerase chain reaction (PCR using previously described TYLCV-specific primer pair TYLCV-Sar/TYLCV-Isr or universal primer pair Begomo-F/Begomo-R. The PCR tests using the primer pair TYLCV-Sar/TYLCV-Isr resulted in the amplification of the expected fragments of ca. 0.67-kb in size for ELISA-positive samples tested from alfalfa, pepper, spinach and tomato plants, confirming the presence of TYLCV. For one melon sample, having a week reaction in ELISA and no reaction in PCR using TYLCV-specific primers, the PCR reaction using the primer pair Begomo-F/Begomo-R resulted in the amplification fragments of the expected size of ca. 2.8 kb. The nucleotide sequences of the DNA amplicons derived from the isolate, Kz-Me198, were determined and compared with other sequences available in GenBank. BLASTN analysis confirmed the begomovirus infection of the sample and showed 99% identities with Tomato leaf curl New Delhi virus (ToLCNDV; phylogenetic analysis supported the results of the database searches. This study reports the natural occurrence of TYLCV in different hosts in Iran. Our results also reveal the emergence of ToLCNDV in Iranian cucurbit crops.

  19. Detection of tomato yellow leaf curl Thailand virus by PCR without DNA extraction.

    Science.gov (United States)

    Ieamkhang, Supaporn; Riangwong, Lumpueng; Chatchawankanphanich, Orawan

    2005-11-01

    We report the simple and rapid method for detection of tomato yellow leaf curl Thailand virus (TYLCTHV) based on the direct capture of virus particles to the surface of a polymerase chain reaction (PCR) tube. This method allowed PCR without the time-consuming procedures of DNA extraction from infected plant tissue. A small amount of tomato tissue (approximately 10 mg) was ground in extraction buffer to release viruses from plant tissues. The constituents of the plant extract that might inhibit PCR activity were discarded by washing the tube with PBST buffer before adding the PCR mixture to the tube. This method was used for detection of TYLCTHV with plant sap solution diluted up to 1:20,000 and was more sensitive than an enzyme-linked immunosorbent assay (ELISA) method. In addition, this method can be used for detection of TYLCTHV in viruliferous whiteflies. The PCR tubes with captured TYLCTHV could be used for PCR, after storage at 4 degrees C for 4 wk. The method presented here was used for detection of begomoviruses in cucurbit and pepper. In addition, this method was effectively used to detect papaya ringspot virus in papaya and zucchini yellow mosaic virus in cucumber by reverse transcriptase (RT)-PCR.

  20. Genetic diversity, host range, and distribution of tomato yellow leaf curl virus in Iran.

    Science.gov (United States)

    Shirazi, M; Mozafari, J; Rakhshandehroo, F; Shams-Bakhsh, M

    2014-01-01

    Tomato yellow leaf curl virus (TYLCV) is considered one of the most important tomato pathogens in tropical and subtropical regions including Iran. During the years 2007 to 2009, a total number of 510 symptomatic and asymptomatic vegetable, ornamental and weed samples were collected from fields and greenhouses in ten provinces of Iran. Symptoms included stunting, yellowing, leaf curl and flower senescence. PCR with specific primers showed TYLCV infection in 184 samples (36%) such as cucumber, pepper, tomato and several weeds from seven provinces. Based on the geographical origin, host range and symptoms, twenty three representative isolates were selected for phylogenetic analysis. An amplicon with a size about 608 base pair (bp) comprising partial sequence of the coat (CP) and movement protein (MP) coding regions of the viral genome was sequenced and compared with the corresponding selected sequences available in GenBank for Iran and worldwide. Phylogenetic analyses on the basis of the nucleotide sequences indicated two geographically separated clades. Isolates collected from Hormozgan, Khuzestan and Kerman provinces were grouped together with other Iranian isolates including TYLCV-Ir2, TYLCV-Kahnooj, and an isolate from Oman. It was also revealed that isolates collected from Boushehr, Fars, Tehran, and Isfahan placed close to the Iranian isolate TYLCV-Abadeh and isolates from Israel and Egypt. No correlation was found between the genetic variation and the host species, but selected Iranian isolates were grouped on the basis of the geographical origins. Results of this study indicated a high genetic diversity among Iranian TYLCV isolates.

  1. Tomato yellow leaf curl virus can be acquired and transmitted by Bemisia tabaci (Gennadius) from tomato fruits

    NARCIS (Netherlands)

    Delatte, H.; Dalmon, A.; Rist, D.; Soustrade, I.; Wuster, G.; Lett, J.M.; Goldbach, R.W.; Peterschmitt, M.; Reynaud, B.

    2003-01-01

    The whitefly Bemisia tabaci is an insect pest causing worldwide economic losses, especially as a vector of geminiviruses such as Tomato yellow leaf curl virus (TYLCV). Currently, imported and exported tomato fruit are not monitored for TYLCV infection because they are not considered to represent a

  2. Characterization of Cestrum yellow leaf curling virus: a new member of the family Caulimoviridae.

    Science.gov (United States)

    Stavolone, Livia; Ragozzino, Antonio; Hohn, Thomas

    2003-12-01

    Cestrum yellow leaf curling virus (CmYLCV) has been characterized as the aetiological agent of the Cestrum parqui mosaic disease. The virus genome was cloned and the clone was proven to be infectious to C. parqui. The presence of typical viroplasms in virus-infected plant tissue and the information obtained from the complete genomic sequence confirmed CmYLCV as a member of the Caulimoviridae family. All characteristic domains conserved in plant pararetroviruses were found in CmYLCV. Its genome is 8253 bp long and contains seven open reading frames (ORFs). Phylogenetic analysis of the relationships with other members of the Caulimoviridae revealed that CmYLCV is closely related to the Soybean chlorotic mottle virus (SbCMV)-like genus and particularly to SbCMV. However, in contrast to the other members of this genus, the primer-binding site is located in the intercistronic region following ORF Ib rather than within this ORF, and an ORF corresponding to ORF VII is missing.

  3. The Involvement of Heat Shock Proteins in the Establishment of Tomato Yellow Leaf Curl Virus Infection

    Science.gov (United States)

    Gorovits, Rena; Czosnek, Henryk

    2017-01-01

    Tomato yellow leaf curl virus (TYLCV), a begomovirus, induces protein aggregation in infected tomatoes and in its whitefly vector Bemisia tabaci. The interactions between TYLCV and HSP70 and HSP90 in plants and vectors are necessity for virus infection to proceed. In infected host cells, HSP70 and HSP90 are redistributed from a soluble to an aggregated state. These aggregates contain, together with viral DNA/proteins and virions, HSPs and components of the protein quality control system such as ubiquitin, 26S proteasome subunits, and the autophagy protein ATG8. TYLCV CP can form complexes with HSPs in tomato and whitefly. Nonetheless, HSP70 and HSP90 play different roles in the viral cell cycle in the plant host. In the infected host cell, HSP70, but not HSP90, participates in the translocation of CP from the cytoplasm into the nucleus. Viral amounts decrease when HSP70 is inhibited, but increase when HSP90 is downregulated. In the whitefly vector, HSP70 impairs the circulative transmission of TYLCV; its inhibition increases transmission. Hence, the efficiency of virus acquisition by whiteflies depends on the functionality of both plant chaperones and their cross-talk with other protein mechanisms controlling virus-induced aggregation.

  4. Identification of a bi-directional promoter from Tomato yellow leaf curl China virus

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A bi-directional promoter of Tomato yellow leaf curl China virus (TYLCCNV) was obtained with the total DNA from TYLCCNV isolate Y10 infected tobacco leaves as a template. Plant expression vectors were constructed by fusing the amplified DNA fragment with the gus gene and nopaline terminator in different orientations. The vectors containing promoter fragments were transferred into leaf cells and plant stems of Nicotiana benthamiana by Agrobacterium-mediated method. Transient expression results showed that both the complementary and virion-sense promoters could drive the gus gene to express, and the GUS activity of the complementary-sense promoter was stronger than that of the virion-sense. Co-expression of the vector containing βC1 gene of TYLCCNV DNAβ with the vector containing a bi-directional promoter revealed that the βC1 protein has no impact on expression of either the virion- or the complementarysense promoter.

  5. Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato

    OpenAIRE

    Yang, X.; Caro Rios, C.M.; Hutton, S.F.; Scott, J. W.; Guo, Y.; Wang, Xiaoxuan; Rashid, H.; Szinay, D.; de Jong; Visser, R.G.F.; Bai, Y.; Du, Y.

    2014-01-01

    Resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (TYLCV), have been introgressed to cultivated tomato (Solanum lycopersicum) from wild tomato accessions. A major gene, Ty-2 from S. habrochaites f. glabratum accession “B6013,” that confers resistance to TYLCV was previously mapped to a 19-cM region on the long arm of chromosome 11. In the present study, approximately 11,000 plants were screened and nearly 157 recombination even...

  6. Impact of Wheat streak mosaic virus and Triticum mosaic virus co-infection of wheat on transmission rates by wheat curl mites

    Science.gov (United States)

    Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are transmitted by the wheat curl mite (WCM, Aceria tosichella Keifer). Previous work has shown that different mite genotypes transmit TriMV at different rates. The objective of this research was to determine if mite genotypes differ...

  7. Efficient Regeneration and Selection of Virus-free Sweetpotato Plants from Sweet Potato Leaf Curl Virus Infected Materials and Their Effects on Yields in Field Trials

    Science.gov (United States)

    Sweet potato leaf curl virus (SPLCV) is an emerging virus disease in sweetpotato (Ipomoea batata) in the U.S. The incidence of SPLCV infection on sweetpotato increased dramatically in recent years due to the explosion of whitefly, Bemisia tabaci (Gennadius) populations. Among several sweetpotato v...

  8. Inhibition of Tomato Yellow Leaf Curl Virus (TYLCV using whey proteins

    Directory of Open Access Journals (Sweden)

    Dawood Abdelgawad

    2010-02-01

    Full Text Available Abstract The antiviral activity of native and esterified whey proteins fractions (α-lactalbumin, β-lactoglobulin, and lactoferrin was studied to inhibit tomato yellow leaf curl virus (TYLCV on infected tomato plants. Whey proteins fractions and their esterified derivatives were sprayed into TYLCV-infected plants. Samples were collected from infected leaves before treatment, 7 and 15 days after treatment for DNA and molecular hybridization analysis. The most evident inhibition of virus replication was observed after 7 and 15 days using α-lactoferrin and α-lactalbumin, respectively. Native and esterified lactoferrin showed complete inhibition after 7 days. On the other hand, native β-lactoglobulin showed inhibition after 7 and 15 days whereas esterified β-lactoglobulin was comparatively more effective after 7 days. The relative amount of viral DNA was less affected by the esterified α-lactalbumin whereas native α-lactalbumin inhibited virus replication completely after 15 days. These results indicate that native or modified whey proteins fractions can be used for controlling the TYLCV-infected plants.

  9. Squash leaf curl virus (SLCV): a serious disease threatening cucurbits production in Palestine.

    Science.gov (United States)

    Ali-Shtayeh, M S; Jamous, R M; Hussein, E Y; Mallah, O B; Abu-Zeitoun, S Y

    2014-04-01

    The incidence of squash leaf curl disease and molecular characterization of the Palestinian isolate of Squash leaf curl virus [SLCV-(PAL)] are described in this study. Symptomatic leaf samples obtained from squash (Cucurbita pepo), watermelon [Citrullus lanatus (Thunb.)], and cucumber (Cucumis sativus L.) plants were tested for SLCV-[PAL] infection by PCR and RCA. SLCV was also found to occur naturally in Chenopodium murale, Convolvulus sp, and Prosporis farcta which showed yellowing. The disease incidence was 85 % in samples collected from Nablus in summer season, while it was 98 % in samples collected from Qalqilia in autumn. On the other hand, SLCV incidence did not exceed 25 % in winter season. The full-length DNA-A and DNA-B genomes of SLCV-[PAL] were amplified and sequenced, and the sequences were deposited in the GenBank. Sequence analysis reveals that SLCV-[PAL] is closely related to other isolates from Lebanon (SLCV-LB2), Jordan (SLCV-JO), Israel (SLCV-IL), and Egypt (SLCV-EG). DNA-A of SLCV-[PAL] showed the highest nucleotide identity (99.4 %) with SLCV-JO, and SLCV-LB2, while DNA-B had the highest nucleotide identity (99.3 %) with SLCV-IL. However, following genome sequencing, it was found that due to two separate point mutations, two viral open reading frames (ORF) were altered in some SLCV Palestinian isolates. The AC2 ORF was extended by 141 nucleotides, while the AC4 ORF was extended by 36 nucleotides.

  10. Detection and Host Range Study of Virus Associated with Pepper Yellow Leaf Curl Disease

    Directory of Open Access Journals (Sweden)

    SRI SULANDARI

    2006-03-01

    Full Text Available High incidence of Pepper yellow leaf curl virus (PepYLCV was observed in Indonesia since early 2000. Disease incidence in Yogyakarta, Central and West Java reached 100% on Capsicum frutescens, but only 10-35% on C. annuum. As an exception, the disease incidence on C. annuum cv. TM 999 was in the range of 70-100%. The causal agent of the disease, PepYLCV, was detected by polymerase chain reaction. Viral specific DNA fragment of the size ~1600 bp and ~550 bp was amplified from infected plants using two pairs of geminivirus universal primers pAL1v1978/pAL1c715, and pAv494/pAc1048, respectively. The PepYLCV has an intermediate host range including plants belonging to the family of Solanaceae, Leguminosae, and Compositae. The species belonging to the families of Cucurbitaceae, Malvaceae, Chenopodiaceae, and Amaranthaceae were resistant to the virus. Physalis floridana, is very prospective as a propagation host for the geminivirus infecting pepper. Nicotiana spp., cucumber, watermelon, cotton, and Sida sp. could be used as a differential host. Besides, Capsicum frutescens cv. Cakra, tomato, N. benthamiana, N. glutinosa, and Ageratum conyzoides could be used as indicator plants for the geminivirus infecting pepper.

  11. Rapid detection of squash leaf curl virus by loop-mediated isothermal amplification.

    Science.gov (United States)

    Kuan, Cheng-Ping; Wu, Min-Tze; Lu, Yi-Lin; Huang, Hung-Chang

    2010-10-01

    A loop-mediated isothermal amplification (LAMP) assay was employed to develop a simple and efficient system for the detection of squash leaf curl virus (SLCV) in diseased plants of squash (Cucurbita pepo) and melon (Cucumis melo). Completion of LAMP assay required 30-60 min under isothermal conditions at 65 degrees C by employing a set of four primers targeting SLCV. Although the sensitivity of the LAMP assay and the polymerase chain reaction (PCR) assay was comparable at high virus concentrations, the LAMP assay was by a 10-fold dilution factor more sensitive than the PCR assay for the detection of SLCV in diseased plants. No reaction was detected in the tissues of healthy plants by either the LAMP or the PCR. The LAMP products can be visualized by staining directly in the tube with SYBR Safe DNA gel stain dye. The sensitivity of the SYBR Safe DNA gel stain is similar to analysis by gel electrophoresis. Although both the LAMP and the PCR methods were capable of detecting SLCV in infected tissues of squash and melon, the LAMP method would be more useful than the PCR method for detection of SLCV infection in cucurbitaceous plants because it is more rapid, simple, accurate and sensitive.

  12. Detection and quantitation of the new world Squash leaf curl virus by TaqMan real-time PCR.

    Science.gov (United States)

    Abrahamian, Peter E; Abou-Jawdah, Yusuf

    2013-07-01

    Squash leaf curl diseases are caused by distinct virus species that are separated into two major phylogenetic groups, western and eastern hemisphere groups. The western group includes the new world Squash leaf curl virus (SLCV) which causes major losses to cucurbit production and induces severe stunting and leaf curl in squash plants. A TaqMan-based real time polymerase chain reaction (qPCR) assay has been developed for detection and quantitation of SLCV. Designed primers and probe targeted the AV1 (coat protein) gene and in silico analysis showed that they detect a large number of SLCV isolates. The developed assay could detect the virus in 18fg of total nucleic acid and 30 genomic units. The qPCR assay was about 1000 times more sensitive than PCR and amplified successfully SLCV from a wide range of cucurbit hosts and from viruliferous whiteflies. The developed qPCR assay should be suitable for detection and quantitation purposes for all reported SLCV isolates of the western hemisphere.

  13. Recessive Resistance Derived from Tomato cv. Tyking-Limits Drastically the Spread of Tomato Yellow Leaf Curl Virus

    Directory of Open Access Journals (Sweden)

    Rita C. Pereira-Carvalho

    2015-05-01

    Full Text Available The tomato yellow leaf curl disease (TYLCD causes severe damage to tomato (Solanum lycopersicum L. crops throughout tropical and subtropical regions of the world. TYLCD is associated with a complex of single-stranded circular DNA plant viruses of the genus Begomovirus (family Geminiviridae transmitted by the whitefy Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae. The tomato inbred line TX 468-RG is a source of monogenic recessive resistance to begomoviruses derived from the hybrid cv. Tyking F1. A detailed analysis of this germplasm source against tomato yellow leaf curl virus-Israel (TYLCV-IL, a widespread TYLCD-associated virus, showed a significant restriction to systemic virus accumulation even under continuous virus supply. The resistance was effective in limiting the onset of TYLCV-IL in tomato, as significantly lower primary spread of the virus occurred in resistant plants. Also, even if a limited number of resistant plants could result infected, they were less efficient virus sources for secondary spread owing to the impaired TYLCV-IL accumulation. Therefore, the incorporation of this resistance into breeding programs might help TYLCD management by drastically limiting TYLCV-IL spread.

  14. Deletional analysis of functional regions of complementary sense promoter from cotton leaf curl virus

    Institute of Scientific and Technical Information of China (English)

    谢迎秋; 刘玉乐; 朱祯

    2000-01-01

    Complementary sense promoter from cotton leaf curl virus (CLCuV) is a novel plant promoter for genetic engineering that could drive high-level foreign gene expression in plant. To determine the optimal promoter sequence for gene expression, CLCuV promoter was deleted from its 5’ end to form promoter fragments with five different lengths, and chimeric gus genes were constructed using the promoterdeletion. These vectors were delivered into Agrobacterium and tobacco (Nicotiana tabacum L cv. Xanthi) plants which were transformed by leaf discs method. GUS activity of transgenic plants was measured. The results showed that GUS activities with the promoter deleted to -287 and -271 from the translation initiation site were respectively about five and three times that of full-length promoter. There exists a c/s-element which is important for the expressing activity in phloem from -271 to -176. Deletion from -176 to -141 resulted in a 20-30-fold reduction in GUS activity in leaves with weak activity in leaves and

  15. Deletional analysis of functional regions of complementary sense promoter from cotton leaf curl virus

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Complementary sense promoter from cotton leaf curl virus (CLCuV) is a novel plant promoter for genetic engineering that could drive high-level foreign gene expression in plant. To determine the optimal promoter sequence for gene expression, CLCuV promoter was deleted from its 5' end to form promoter fragments with five different lengths, and chimeric gus genes were constructed using the promoter deletion. These vectors were delivered into Agrobacterium and tobacco (Nicotiana tabacum L. cv. Xanthi) plants which were transformed by leaf discs method. GUS activity of transgenic plants was measured. The results showed that GUS activities with the promoter deleted to -287 and -271 from the translation initiation site were respectively about five and three times that of full-length promoter. There exists a cis-element which is important for the expressing activity in phloem from -271 to -176. Deletion from -176 to -141 resulted in a 20-30-fold reduction in GUS activity in leaves with weak activity in leaves and stems and losing GUS activity in roots. The functional domains of complementary sense gene promoter of CLCuV were firstly analyzed and compared. It was found that the promoter activity with the deletion of negative cis-elements was much stronger than that of full-length promoter and was about twelve times on average that of CaMV 35S promoter, suggesting that the promoter has great application potential. Results also provide novel clues for understanding the mechanisms of geminivirus gene regulation and interaction between virus and plant.

  16. Characterization of a synergistic interaction between two cucurbit-infecting begomoviruses: Squash leaf curl virus and Watermelon chlorotic stunt virus.

    Science.gov (United States)

    Sufrin-Ringwald, Tali; Lapidot, Moshe

    2011-02-01

    Squash leaf curl virus (SLCV) and Watermelon chlorotic stunt virus (WmCSV) are cucurbit-infecting bipartite begomoviruses. Both viruses are found in the eastern Mediterranean basin but the effects of dual infection of both viruses on melon (Cucumis melo L.) have not been described. 'Arava' melon plants were inoculated in the greenhouse, using whiteflies, with either SLCV, WmCSV, or both. Control plants were exposed to nonviruliferous whiteflies or not exposed at all. Following inoculation, plants were transplanted to a 50-mesh insect-proof nethouse and grown until fruit maturity. The experiment was performed in two melon-growing seasons: spring, transplant in May and harvest in July; and summer, transplant in August and harvest in October. Following inoculation, SLCV-infected melon plants showed mild symptoms that disappeared with time, and there was no effect on plant height. WmCSV-infected plants developed disease symptoms that became more obvious with time, and plants were somewhat shorter than control plants in the spring but not in the summer. SLCV had no effect on yield, regardless of season. WmCSV had no statistically significant effect on yield in the spring but, in the summer, reduced yield by 22%, on average. Dual-inoculated plants showed a synergistic interaction between the two viruses. They developed disease symptoms that were more pronounced than WmCSV alone, with plants being shorter than control plants by 20 to 25% regardless of season. Moreover, the yield of dual-inoculated plants was reduced on average by 21% in the spring and 54% in the summer, and fruit appearance was adversely affected. Dual inoculation did not affect WmCSV DNA level but SLCV DNA level was increased several-fold by the presence of WmCSV.

  17. Molecular and biological characterization of a new Tomato mild yellow leaf curl Aragua virus strain producing severe symptoms in tomato.

    Science.gov (United States)

    Romay, Gustavo; Chirinos, Dorys T; Geraud-Pouey, Francis; Gillis, Annika; Mahillon, Jacques; Desbiez, Cécile; Bragard, Claude

    2017-06-20

    Tomato mild yellow leaf curl Aragua virus (ToMYLCV) is a begomovirus first reported infecting tomato (Solanum lycopersicum) and milkweed (Euphorbia heterophylla) in Venezuela. In this study, a ToMYLCV isolate (Zulia-219) was completely sequenced and its host range was evaluated. The DNA-A and DNA-B components of isolate Zulia-219 showed 93 and 85% nucleotide sequence identity with the respective counterparts of the ToMYLCV type strain. According to current demarcation criteria for begomovirus species, Zulia-219 is a new strain of ToMYLCV. Interestingly, tomato plants inoculated with ToMYLCV Zulia-219 displayed severe symptoms, including severe chlorotic leaf curling, in contrast to mild symptoms associated with the type strain of this begomovirus. These results indicate potential risks associated with this new ToMYLCV strain for tomato production in Venezuela.

  18. Association of an alphasatellite with tomato yellow leaf curl virus and ageratum yellow vein virus in Japan is suggestive of a recent introduction.

    Science.gov (United States)

    Shahid, Muhammad Shafiq; Ikegami, Masato; Waheed, Abdul; Briddon, Rob W; Natsuaki, Keiko T

    2014-01-14

    Samples were collected in 2011 from tomato plants exhibiting typical tomato leaf curl disease symptoms in the vicinity of Komae, Japan. PCR mediated amplification, cloning and sequencing of all begomovirus components from two plants from different fields showed the plants to be infected by Tomato yellow leaf curl virus (TYLCV) and Ageratum yellow vein virus (AYVV). Both viruses have previously been shown to be present in Japan, although this is the first identification of AYVV on mainland Japan; the virus previously having been shown to be present on the Okinawa Islands. The plant harboring AYVV was also shown to contain the betasatellite Tomato leaf curl Java betasatellite (ToLCJaB), a satellite not previously shown to be present in Japan. No betasatellite was associated with the TYLCV infected tomato plants analyzed here, consistent with earlier findings for this virus in Japan. Surprisingly both plants were also found to harbor an alphasatellite; no alphasatellites having previously been reported from Japan. The alphasatellite associated with both viruses was shown to be Sida yellow vein China alphasatellite which has previously only been identified in the Yunnan Province of China and Nepal. The results suggest that further begomoviruses, and their associated satellites, are being introduced to Japan. The significance of these findings is discussed.

  19. Advances of research on Cotton leaf curl virus%棉花曲叶病毒研究进展

    Institute of Scientific and Technical Information of China (English)

    丁小兰; 赵竹; 李明福

    2013-01-01

    The Cotton leaf curl virus as a quarantine virus in China is severely harmful to the production of the cotton. It caused destructive damage on cotton production in Pakistan and India. The virus could be transimitted by the whitefly and had a wide host range. In 2006, the Cotton leaf curl virus was found to infect Hibiscus rosa -sinensis in Guangdong. Moreover, in 2010, CLCuV was reported to be associated with cotton leaf curl disease in a experimental field of Nanning, Guangxi province in China. Cotton is an important crop in China, once the virus is spread to the main cotton producing areas, the consequences would be disastrous. In this paper, the variants of the virus, distribution and popular trend, disease epidemiology, the influence of CLCuV on cotton production and quality traits and disease control are analysed. In addition, the interesting issues and effective management measures for CLCuV were discussed also.%棉花曲叶病毒(Cotton leaf curl virus,CLCuV)是我国的进境检疫性有害生物,严重危害棉花生长,在巴基斯坦和印度的棉花产区已造成毁灭性灾害.该病毒主要由烟粉虱传播,寄主范围广泛.2006年在广东首次发现棉花曲叶病毒(CLCuV)入侵我国危害朱槿,2010年有报道证实CLCuV已侵染广西南宁试验田的棉花.我国是棉花生产大国,该病毒一旦扩散传播到棉花主产区,后果将不堪设想.本文对CLCuV的变异、国内外分布和流行趋势、影响棉花曲叶病流行的因素、病毒对棉花的品质和产量的影响及病害治理等方面进行综述,并探讨了棉花曲叶病毒研究存在的问题及有效防止该病毒传播和扩散的措施.

  20. Melon chlorotic leaf curl virus: characterization and differential reassortment with closest relatives reveal adaptive virulence in the squash leaf curl virus clade and host shifting by the host-restricted bean calico mosaic virus.

    Science.gov (United States)

    Idris, A M; Mills-Lujan, K; Martin, K; Brown, J K

    2008-02-01

    The genome components of the Melon chlorotic leaf curl virus (MCLCuV) were cloned from symptomatic cantaloupe leaves collected in Guatemala during 2002. The MCLCuV DNA-A and DNA-B components shared their closest nucleotide identities among begomoviruses, at approximately 90 and 81%, respectively, with a papaya isolate of MCLCuV from Costa Rica. The closest relatives at the species level were other members of the Squash leaf curl virus (SLCV) clade, which is endemic in the southwestern United States and Mexico. Biolistic inoculation of cantaloupe seedlings with the MCLCuV DNA-A and -B components resulted in the development of characteristic disease symptoms, providing definitive evidence of causality. MCLCuV experimentally infected species within the Cucurbitaceae, Fabaceae, and Solanaceae. The potential for interspecific reassortment was examined for MCLCuV and its closest relatives, including the bean-restricted Bean calico mosaic virus (BCaMV), and three other cucurbit-infecting species, Cucurbit leaf crumple virus (CuLCrV), SLCV, and SMLCV. The cucurbit viruses have distinct but overlapping host ranges. All possible reassortants were established using heterologous combinations of the DNA-A or DNA-B components. Surprisingly, only certain reassortants arising from MCLCuV and BCaMV, or MCLCuV and CuLCrV, were viable in bean, even though it is a host of all of the "wild-type" (parent) viruses. The bean-restricted BCaMV was differentially assisted in systemically infecting the cucurbit test species by the components of the four cucurbit-adapted begomoviruses. In certain heterologous combinations, the BCaMV DNA-A or -B component was able to infect one or more cucurbit species. Generally, the reassortants were less virulent in the test hosts than the respective wild-type (parent) viruses, strongly implicating adaptive modulation of virulence. This is the first illustration of reassortment resulting in the host range expansion of a host-restricted begomovirus.

  1. Philippines.

    Science.gov (United States)

    1986-08-01

    The Philippines is an archipelago of 7100 islands and islets, 11 of which compose about 95% of the total area and population. The majority of the Filipinos are descendants of Indonesians and Malays. Approximately 90% of the population are Christian with the majority of the remaining 10% being Moslems. In the 1960s, the annual population growth rate was roughly 3%, but it fell to 2.4% in the late 1970s and was still 2.4% in 1985. In 1970, President Marcos implemented an official family planning policy to reduce the high growth rate and thereby stimulate economic development. A population commission coordinates family planning efforts. Both the Spanish (1521-1898) and the United States (1898-1946) have ruled the Philippines with a brief occupation by the Japanese (1942-1945). The US assisted in the reconstruction of the economy following World War II and continues to maintain and operate military bases. Further, from 1946-1986, the Philippines has received $3.7 billion in economic and military assistance from the US. The government operated under a constitutional democracy from 1946-1972, but in 1972 President Marcos declared martial law. In 1981, martial law ended and Marcos called for a presidential election. After winning the election, he called for an amendment of the 1972 constitution making him, rather than the prime minister, the head of government. Even though martial law ended in 1981, the Marcos government retained its wide powers to arrest and detain anyone. In February 1986, popular support backed by a peaceful civilian-military uprising brought Corazon Aquino to the Presidency. In the mid 1980s a severe economic recession hit the Philippines with the real GNP growth rate ranging from -5.3%-0%. The Philippines have diplomatic relations with the south east Asian nations, many East Bloc nations, the US, China, Cuba, and the Soviet Union.

  2. Amplicon based RNA interference targeting V2 gene of cotton leaf curl Kokhran virus-Burewala strain can provide resistance in transgenic cotton plants

    Science.gov (United States)

    An RNAi based gene construct designated “C2” was used to target the V2 region of the cotton leaf curl virus (CLCuV) genome which is responsible for virus movement. The construct was transformed into two elite cotton varieties MNH-786 and VH-289. A shoot apex method of plant transformation using Agr...

  3. Evaluation under diverse conditions of a differential host reaction scale to Tomato yellow leaf curl virus in tomato

    Directory of Open Access Journals (Sweden)

    Ana PÉREZ-DE-CASTRO

    2017-05-01

    Full Text Available Tomato yellow leaf curl virus (TYLCV is the most widespread virus species causing Tomato yellow leaf curl disease. Accurate methods for assessment of resistance are requisite in breeding programs. Researchers at the Volcani Center (Israel developed a scale of differential TYLCV reactions in seven non-segregating tomato host lines, with virus responses ranging from susceptible to highly resistant. In the present work, this scale has been evaluated with inoculation and assessment methods routinely used by two different research groups, in Spain and Cuba. Different TYLCV isolates, inoculation methods, plant ages, and environmental conditions were compared. Symptom scores of the tomato lines were generally lower in the conditions assayed in both locations than those originally described. Reaction ranking order of the standard tomato lines was more similar to the original description in the assays carried out in Spain. However, response of the lines to TYLCV obtained in both locations did not correspond to scale grades for most of the conditions. A large difference between symptom scores in the susceptible and the resistant lines was observed, with the range in the resistant lines being narrow. These results indicate that the number of standard lines used could be reduced, selecting the most susceptible and the most resistant lines, and one with intermediate resistance. All the factors evaluated affected symptom development. This highlights the importance of establishing a standard inoculation method, experimental conditions, evaluation period and appropriate resistance evaluation criteria, to ensure precise evaluation of genotype responses.

  4. Flupyradifurone effectively manages whitefly Bemisia tabaci MED (Hemiptera: Aleyrodidae) and tomato yellow leaf curl virus in tomato.

    Science.gov (United States)

    Roditakis, Emmanouil; Stavrakaki, Marianna; Grispou, Maria; Achimastou, Aikaterini; Van Waetermeulen, Xavier; Nauen, Ralf; Tsagkarakou, Anastasia

    2017-08-01

    The cotton whitefly Bemisia tabaci (Gennadius) is among the most important pests of numerous crops and a vector of more than 100 plant viruses, causing significant crop losses worldwide. Managing this pest as well as inhibiting the transmission of major viruses such as tomato yellow leaf curl virus (TYLCV) are of utmost importance for sustainable yields. The efficacy against both whitefly and virus transmission of the novel systemic butenolide insecticide flupyradifurone was investigated in this study. The inhibition of TYLCV transmission by flupyradifurone was compared to that by thiamethoxam, a neonicotinoid insecticide reported to inhibit virus transmission. The experiment was performed under high virus pressure conditions (10 viruliferous insects per plant for 48 h) using a fully characterized field strain of B. tabaci. The insecticides were foliarly applied at recommended label rates under greenhouse conditions. Flupyradifurone suppressed virus transmission by 85% while levels of suppression after thiamethoxam treatments were just 25% and significantly lower. In untreated control plots, 100% of plants were infected by TYLCV. The observed difference in the potential to suppress virus transmission is linked to a strong knockdown effect as well as prolonged feeding inhibition in flupyradifurone treatments. Flupyradifurone is shown to be an extremely useful, fast-acting, new chemical tool in integrated crop management offering simultaneous control of whiteflies and strong suppression of viral infections via its rapid knockdown action and good residual activity. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  5. Detection of novel respiratory viruses from influenza-like illness in the Philippines.

    Science.gov (United States)

    Furuse, Yuki; Suzuki, Akira; Kishi, Makiko; Galang, Hazel O; Lupisan, Socorro P; Olveda, Remigio M; Oshitani, Hitoshi

    2010-05-01

    Several novel viruses have been recently identified in respiratory samples. However, the epidemiology of these viruses in tropical countries remains unclear. The aim of the present study was to provide an overview of the epidemiology of novel respiratory viruses, including human metapneumovirus, human bocavirus, new subtypes of human coronavirus (NL63 and HKU1), KI virus, WU virus, and Melaka virus in the Philippines, a tropical country. Nasopharyngeal aspirates from 465 patients with influenza-like illness were collected in 2006 and 2007. Reverse transcription polymerase chain reaction (RT-PCR) and PCR were performed to detect viruses from culture-negative specimens. Human metapneumovirus, human bocavirus, human coronavirus HKU1, KI virus, and WU virus were detected for the first time in the Philippines; Melaka virus was not found.

  6. Genome organization of Tobacco leaf curl Zimbabwe virus, a new, distinct monopartite begomovirus associated with subgenomic defective DNA molecules.

    Science.gov (United States)

    Paximadis, M; Rey, M E

    2001-12-01

    The complete DNA A of the begomovirus Tobacco leaf curl Zimbabwe virus (TbLCZWV) was sequenced: it comprises 2767 nucleotides with six major open reading frames encoding proteins with molecular masses greater than 9 kDa. Full-length TbLCZWV DNA A tandem dimers, cloned in binary vectors (pBin19 and pBI121) and transformed into Agrobacterium tumefaciens, were systemically infectious upon agroinoculation of tobacco and tomato. Efforts to identify a DNA B component were unsuccessful. These findings suggest that TbLCZWV is a new member of the monopartite group of begomoviruses. Phylogenetic analysis identified TbLCZWV as a distinct begomovirus with its closest relative being Chayote mosaic virus. Abutting primer PCR amplified ca. 1300 bp molecules, and cloning and sequencing of two of these molecules revealed them to be subgenomic defective DNA molecules originating from TbLCZWV DNA A. Variable symptom severity associated with tobacco leaf curl disease and TbLCZWV is discussed.

  7. MicroRNA profiling of tomato leaf curl new delhi virus (tolcndv infected tomato leaves indicates that deregulation of mir159/319 and mir172 might be linked with leaf curl disease

    Directory of Open Access Journals (Sweden)

    Haq Qazi MR

    2010-10-01

    Full Text Available Abstract Background Tomato leaf curl virus (ToLCV, a constituent of the genus Begomovirus, infects tomato and other plants with a hallmark disease symptom of upward leaf curling. Since microRNAs (miRs are known to control plants developmental processes, we evaluated the roles of miRNAs in Tomato leaf curl New Delhi virus (ToLCNDV induced leaf curling. Results Microarray analyses of miRNAs, isolated from the leaves of both healthy and ToLCNDV agroinfected tomato cv Pusa Ruby, revealed that ToLCNDV infection significantly deregulated various miRNAs representing ~13 different conserved families (e.g., miR319, miR172, etc.. The precursors of these miRNAs showed similar deregulated patterns, indicating that the transcription regulation of respective miRNA genes was perhaps the cause of deregulation. The expression levels of the miRNA-targeted genes were antagonistic with respect to the amount of corresponding miRNA. Such deregulation was tissue-specific in nature as no analogous misexpression was found in flowers. The accumulation of miR159/319 and miR172 was observed to increase with the days post inoculation (dpi of ToLCNDV agroinfection in tomato cv Pusa Ruby. Similarly, these miRs were also induced in ToLCNDV agroinfected tomato cv JK Asha and chilli plants, both exhibiting leaf curl symptoms. Our results indicate that miR159/319 and miR172 might be associated with leaf curl symptoms. This report raises the possibility of using miRNA(s as potential signature molecules for ToLCNDV infection. Conclusions The expression of several host miRNAs is affected in response to viral infection. The levels of the corresponding pre-miRs and the predicted targets were also deregulated. This change in miRNA expression levels was specific to leaf tissues and observed to be associated with disease progression. Thus, certain host miRs are likely indicator of viral infection and could be potentially employed to develop viral resistance strategies.

  8. New insecticides for management of tomato yellow leaf curl, a virus vectored by the silverleaf whitefly, Bemisia tabaci.

    Science.gov (United States)

    Smith, H A; Giurcanu, M C

    2014-01-01

    Greenhouse studies using a randomized complete block design were carried out to evaluate the effect of six insecticides on transmission of Tomato yellow leaf curl virus (TYLCV) by the silverleaf whitefly, Bemisia tabaci biotype B Gennadius (Hemiptera: Aleyrodidae) to tomato, Lycopersicon esculentum (Miller) (Solanales: Solanaceae), seedlings that were inoculated with whiteflies from a TYLCV colony in cages 3, 7, or 14 d after treatment with insecticide. The purpose was to reveal differences in residual efficacy of four materials that are nearing registration for use on tomato-cyazypyr, flupyradifurone, pyrafluquinazon, and sulfoxaflor-and to compare them with two established insecticides, pymetrozine and a zeta-cypermethrin/bifenthrin combination. Differences in efficacy were expected because these six materials represent five distinct modes of action and both contact and systemic materials. Percentage of tomato seedlings expressing virus symptoms tended to be lowest in seedlings treated with flupyradifurone. The zeta-cypermethrin/bifenthrin insecticide demonstrated comparable efficacy to flupyradifurone in some trials at 3 and 7 d after treatment inoculations, but not the 14 d after treatment inoculation. Pyrafluquinazon was not statistically different from cyazypyr or sulfoxaflor in percentage of plants with virus symptoms in any trial. Percentage virus in the cyazypyr and sulfoxaflor treatments was not statistically different in the 3 and 7 d after treatment inoculations. Among seedlings treated with insecticide, percentage with virus symptoms tended to be highest in the seedlings treated with pymetrozine.

  9. Transcriptome profiling to discover defense-related genes associated with resistance line ty-5 against Tomato yellow leaf curl virus in tomato

    Science.gov (United States)

    Tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted begomovirus, has caused serious economic losses to tomato crops in the U.S. and around the world. The most effective management would be the use of a TYLCV-resistant tomato cultivar. Several sources of TYLCV resistance genes have been ide...

  10. Field evaluation of yield effects on the U.S.A. heirloom sweet potato cultivars infected by sweet potato leaf curl virus

    Science.gov (United States)

    The incidence of Sweet potato leaf curl virus (SPLCV), a Begomovirus, infection of sweetpotato Ipomoea batatas (L.) Lam. (Convolvulaceae) in South Carolina, USA has increased rapidly in recent years. This is likely due to the use of infected propagating materials and the increasing population of it...

  11. Comparison of transmission of Papaya leaf curl China virus among four cryptic species of the whitefly Bemisia tabaci complex

    Science.gov (United States)

    Guo, Tao; Guo, Qi; Cui, Xi-Yun; Liu, Yin-Quan; Hu, Jian; Liu, Shu-Sheng

    2015-01-01

    Begomoviruses are transmitted by cryptic species of the whitefly Bemisia tabaci complex, often in a species-specific manner. Papaya leaf curl China virus (PaLCuCNV) has been recorded to infect several crops including papaya, tomato and tobacco in China. To help assess the risks of spread of this virus, we compared the acquisition, retention and transmission of PaLCuCNV among four species of whiteflies, Middle East-Asia Minor 1 (MEAM1), Mediterranean (MED), Asia 1 and Asia II 7. All four species of whiteflies are able to acquire, retain and transmit the virus, but with different levels of efficiency. Transmission tests using tomato as the host plant showed that MEAM1 transmitted PaLCuCNV with substantially higher efficiency than did MED, Asia 1 and Asia II 7. Furthermore, accumulation of PaLCuCNV in the whiteflies was positively associated with its efficiency of transmitting the virus. Altogether, these findings indicate that MEAM1 is the most efficient vector for PaLCuCNV in the four species of whiteflies, and suggest that risks of PaLCuCNV pandemics are high in regions where MEAM1 occurs. PMID:26486606

  12. Molecular dissection of Tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum.

    Science.gov (United States)

    Anbinder, Ilana; Reuveni, Moshe; Azari, Raviv; Paran, Ilan; Nahon, Sahadia; Shlomo, Haviva; Chen, Lea; Lapidot, Moshe; Levin, Ilan

    2009-08-01

    Tomato yellow leaf curl virus (TYLCV) is devastating to tomato (Solanum lycopersicum) crops and resistant cultivars are highly effective in controlling the disease. The breeding line TY172, originating from Solanum peruvianum, is highly resistant to TYLCV. To map quantitative trait loci (QTLs) controlling TYLCV resistance in TY172, appropriate segregating populations were analyzed using 69 polymorphic DNA markers spanning the entire tomato genome. Results show that TYLCV resistance in TY172 is controlled by a previously unknown major QTL, originating from the resistant line, and four additional minor QTLs. The major QTL, we term Ty-5, maps to chromosome 4 and accounts for 39.7-46.6% of the variation in symptom severity among segregating plants (LOD score 33-35). The minor QTLs, originated either from the resistant or susceptible parents, were mapped to chromosomes 1, 7, 9 and 11, and contributed 12% to the variation in symptom severity in addition to Ty-5.

  13. TaqMan real-time PCR for detection and quantitation of squash leaf curl virus in cucurbits.

    Science.gov (United States)

    Kuan, Cheng-Ping; Huang, Hung-Chang; Chang, Chia-Che; Lu, Yi-Lin

    2012-02-01

    A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the real-time PCR established in this study enabled detection of as little as 10(2) copies of SLCV DNA with CP gene as the target. This TaqMan real-time PCR assay for detection and quantitation of SLCV would be a useful tool for application in quarantine and certification of SLCV in cucurbits as well as in the research of disease resistance and epidemiology.

  14. Host range and genetic diversity of croton yellow vein mosaic virus, a weed-infecting monopartite begomovirus causing leaf curl disease in tomato.

    Science.gov (United States)

    Pramesh, D; Mandal, Bikash; Phaneendra, Chigurupati; Muniyappa, V

    2013-03-01

    Croton yellow vein mosaic virus (CYVMV) is a widely occurring begomovirus in Croton bonplandianum, a common weed in the Indian subcontinent. In this study, CYVMV (genus Begomovirus, family Geminiviridae) was transmitted by whiteflies (Bemisia tabaci) to as many as 35 plant species belonging to 11 families, including many vegetables, tobacco varieties, ornamentals and weeds. CYVMV produced bright yellow vein symptoms in croton, whereas in all the other host species, the virus produced leaf curl symptoms. CYVMV produced leaf curl in 13 tobacco species and 22 cultivars of Nicotiana tabacum and resembled tobacco leaf curl virus (TobLCV) in host reactions. However, CYVMV was distinguished from TobLCV in four differential hosts, Ageratum conyzoides, C. bonplandianum, Euphorbia geniculata and Sonchus bracyotis. The complete genome sequences of four isolates originating from northern, eastern and southern India revealed that a single species of DNA-A and a betasatellite, croton yellow vein mosaic betasatellite (CroYVMB) were associated with the yellow vein mosaic disease of croton. The sequence identity among the isolates of CYVMV DNA-A and CroYVMB occurring in diverse plant species was 91.8-97.9 % and 83.3-100 %, respectively. The CYVMV DNA-A and CroYVMB generated through rolling-circle amplification of the cloned DNAs produced typical symptoms of yellow vein mosaic and leaf curling in croton and tomato, respectively. The progeny virus from both the croton and tomato plants was transmitted successfully by B. tabaci. The present study establishes the etiology of yellow vein mosaic disease of C. bonplandianum and provides molecular evidence that a weed-infecting monopartite begomovirus causes leaf curl in tomato.

  15. Implication of the bacterial endosymbiont Rickettsia spp. in interactions of the whitefly Bemisia tabaci with tomato yellow leaf curl virus.

    Science.gov (United States)

    Kliot, Adi; Cilia, Michelle; Czosnek, Henryk; Ghanim, Murad

    2014-05-01

    Numerous animal and plant viruses are transmitted by arthropod vectors in a persistent, circulative manner. Tomato yellow leaf curl virus (TYLCV) is transmitted by the sweet potato whitefly Bemisia tabaci. We report here that infection with Rickettsia spp., a facultative endosymbiont of whiteflies, altered TYLCV-B. tabaci interactions. A B. tabaci strain infected with Rickettsia acquired more TYLCV from infected plants, retained the virus longer, and exhibited nearly double the transmission efficiency compared to an uninfected B. tabaci strain with the same genetic background. Temporal and spatial antagonistic relationships were discovered between Rickettsia and TYLCV within the whitefly. In different time course experiments, the levels of virus and Rickettsia within the insect were inversely correlated. Fluorescence in situ hybridization analysis of Rickettsia-infected midguts provided evidence for niche exclusion between Rickettsia and TYLCV. In particular, high levels of the bacterium in the midgut resulted in higher virus concentrations in the filter chamber, a favored site for virus translocation along the transmission pathway, whereas low levels of Rickettsia in the midgut resulted in an even distribution of the virus. Taken together, these results indicate that Rickettsia, by infecting the midgut, increases TYLCV transmission efficacy, adding further insights into the complex association between persistent plant viruses, their insect vectors, and microorganism tenants that reside within these insects. Interest in bacterial endosymbionts in arthropods and many aspects of their host biology in agricultural and human health systems has been increasing. A recent and relevant studied example is the influence of Wolbachia on dengue virus transmission by mosquitoes. In parallel with our recently studied whitefly-Rickettsia-TYLCV system, other studies have shown that dengue virus levels in the mosquito vector are inversely correlated with bacterial load. Our work

  16. Genetic diversity and geographic distribution of genetically distinct rabies viruses in the Philippines.

    Science.gov (United States)

    Saito, Mariko; Oshitani, Hitoshi; Orbina, Jun Ryan C; Tohma, Kentaro; de Guzman, Alice S; Kamigaki, Taro; Demetria, Catalino S; Manalo, Daria L; Miranda, Mary Elizabeth G; Noguchi, Akira; Inoue, Satoshi; Quiambao, Beatriz P

    2013-01-01

    Rabies continues to be a major public health problem in the Philippines, where 200-300 human cases were reported annually between 2001 and 2011. Understanding the phylogeography of rabies viruses is important for establishing a more effective and feasible control strategy. We performed a molecular analysis of rabies viruses in the Philippines using rabied animal brain samples. The samples were collected from 11 of 17 regions, which covered three island groups (Luzon, Visayas, and Mindanao). Partial nucleoprotein (N) gene sequencing was performed on 57 samples and complete glycoprotein (G) gene sequencing was performed on 235 samples collected between 2004 and 2010. The Philippine strains of rabies viruses were included in a distinct phylogenetic cluster, previously named Asian 2b, which appeared to have diverged from the Chinese strain named Asian 2a. The Philippine strains were further divided into three major clades, which were found exclusively in different island groups: clades L, V, and M in Luzon, Visayas, and Mindanao, respectively. Clade L was subdivided into nine subclades (L1-L9) and clade V was subdivided into two subclades (V1 and V2). With a few exceptions, most strains in each subclade were distributed in specific geographic areas. There were also four strains that were divided into two genogroups but were not classified into any of the three major clades, and all four strains were found in the island group of Luzon. We detected three major clades and two distinct genogroups of rabies viruses in the Philippines. Our data suggest that viruses of each clade and subclade evolved independently in each area without frequent introduction into other areas. An important implication of these data is that geographically targeted dog vaccination using the island group approach may effectively control rabies in the Philippines.

  17. Genetic diversity and geographic distribution of genetically distinct rabies viruses in the Philippines.

    Directory of Open Access Journals (Sweden)

    Mariko Saito

    Full Text Available BACKGROUND: Rabies continues to be a major public health problem in the Philippines, where 200-300 human cases were reported annually between 2001 and 2011. Understanding the phylogeography of rabies viruses is important for establishing a more effective and feasible control strategy. METHODS: We performed a molecular analysis of rabies viruses in the Philippines using rabied animal brain samples. The samples were collected from 11 of 17 regions, which covered three island groups (Luzon, Visayas, and Mindanao. Partial nucleoprotein (N gene sequencing was performed on 57 samples and complete glycoprotein (G gene sequencing was performed on 235 samples collected between 2004 and 2010. RESULTS: The Philippine strains of rabies viruses were included in a distinct phylogenetic cluster, previously named Asian 2b, which appeared to have diverged from the Chinese strain named Asian 2a. The Philippine strains were further divided into three major clades, which were found exclusively in different island groups: clades L, V, and M in Luzon, Visayas, and Mindanao, respectively. Clade L was subdivided into nine subclades (L1-L9 and clade V was subdivided into two subclades (V1 and V2. With a few exceptions, most strains in each subclade were distributed in specific geographic areas. There were also four strains that were divided into two genogroups but were not classified into any of the three major clades, and all four strains were found in the island group of Luzon. CONCLUSION: We detected three major clades and two distinct genogroups of rabies viruses in the Philippines. Our data suggest that viruses of each clade and subclade evolved independently in each area without frequent introduction into other areas. An important implication of these data is that geographically targeted dog vaccination using the island group approach may effectively control rabies in the Philippines.

  18. Phylodynamic reconstruction of O CATHAY topotype foot-and-mouth disease virus epidemics in the Philippines.

    Science.gov (United States)

    Di Nardo, Antonello; Knowles, Nick J; Wadsworth, Jemma; Haydon, Daniel T; King, Donald P

    2014-01-01

    Reconstructing the evolutionary history, demographic signal and dispersal processes from viral genome sequences contributes to our understanding of the epidemiological dynamics underlying epizootic events. In this study, a Bayesian phylogenetic framework was used to explore the phylodynamics and spatio-temporal dispersion of the O CATHAY topotype of foot-and-mouth disease virus (FMDV) that caused epidemics in the Philippines between 1994 and 2005. Sequences of the FMDV genome encoding the VP1 showed that the O CATHAY FMD epizootic in the Philippines resulted from a single introduction and was characterised by three main transmission hubs in Rizal, Bulacan and Manila Provinces. From a wider regional perspective, phylogenetic reconstruction of all available O CATHAY VP1 nucleotide sequences identified three distinct sub-lineages associated with country-based clusters originating in Hong Kong Special Administrative Region (SAR), the Philippines and Taiwan. The root of this phylogenetic tree was located in Hong Kong SAR, representing the most likely source for the introduction of this lineage into the Philippines and Taiwan. The reconstructed O CATHAY phylodynamics revealed three chronologically distinct evolutionary phases, culminating in a reduction in viral diversity over the final 10 years. The analysis suggests that viruses from the O CATHAY topotype have been continually maintained within swine industries close to Hong Kong SAR, following the extinction of virus lineages from the Philippines and the reduced number of FMD cases in Taiwan.

  19. AC2 and AC4 proteins of Tomato yellow leaf curl China virus and Tobacco curly shoot virus mediate suppression of RNA silencing

    Institute of Scientific and Technical Information of China (English)

    CUI Xiaofeng; ZHOU Xueping

    2004-01-01

    Tomato yellow leaf curl China virus Y10 isolate (TYLCCNV-Y10) alone could systemically infect host plants such as Nicotiana benthamiana without symptoms. In contrast, Tobacco curly shoot virus Y35 isolate (TbCSV-Y35) alone induces leaf curl symptoms in N. benthamiana. When inoculated into transgenic N. benthamiana plants expressing GFP gene (line 16c), TYLCCNV-Y10 neither reverses the established GFP silencing nor blocks the onset of GFP silencing. In contrast, TbCSV-Y35 can partially reverse the established GFP silencing and block the onset of GFP silencing in new leaves. In the patch co-infiltration assays, the AC2 and AC4 proteins of TYLCCNV-Y10 and TbCSV-Y35 could suppress local GFP silencing and delay systemic GFP silencing, suggesting that they are suppressors of RNA silencing. Comparison of the accumulation levels of GFP mRNA in the co-infiltration patches showed that Y10 AC2 and Y35 AC2 proteins had similar efficiency for suppression of RNA silencing. However, Y35 AC4 protein functioned as a stronger suppressor of RNA silencing than Y10 AC4 protein. Therefore, the pathogenicity difference between TbCSV-Y35 and TYLCCNV-Y10 may be related to the functional difference in their AC4 proteins.

  20. Rapid accumulation and low degradation: key parameters of Tomato yellow leaf curl virus persistence in its insect vector Bemisia tabaci.

    Science.gov (United States)

    Becker, Nathalie; Rimbaud, Loup; Chiroleu, Frédéric; Reynaud, Bernard; Thébaud, Gaël; Lett, Jean-Michel

    2015-12-02

    Of worldwide economic importance, Tomato yellow leaf curl virus (TYLCV, Begomovirus) is responsible for one of the most devastating plant diseases in warm and temperate regions. The DNA begomoviruses (Geminiviridae) are transmitted by the whitefly species complex Bemisia tabaci. Although geminiviruses have long been described as circulative non-propagative viruses, observations such as long persistence of TYLCV in B. tabaci raised the question of their possible replication in the vector. We monitored two major TYLCV strains, Mild (Mld) and Israel (IL), in the invasive B. tabaci Middle East-Asia Minor 1 cryptic species, during and after the viral acquisition, within two timeframes (0-144 hours or 0-20 days). TYLCV DNA was quantified using real-time PCR, and the complementary DNA strand of TYLCV involved in viral replication was specifically quantified using anchored real-time PCR. The DNA of both TYLCV strains accumulated exponentially during acquisition but remained stable after viral acquisition had stopped. Neither replication nor vertical transmission were observed. In conclusion, our quantification of the viral loads and complementary strands of both Mld and IL strains of TYLCV in B. tabaci point to an efficient accumulation and preservation mechanism, rather than to a dynamic equilibrium between replication and degradation.

  1. Functional characterization of a strong bi-directional constitutive plant promoter isolated from cotton leaf curl Burewala virus.

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    Zainul A Khan

    Full Text Available Cotton leaf curl Burewala virus (CLCuBuV, belonging to the genus Begomovirus, possesses single-stranded monopartite DNA genome. The bidirectional promoters representing Rep and coat protein (CP genes of CLCuBuV were characterized and their efficacy was assayed. Rep and CP promoters of CLCuBuV and 35S promoter of Cauliflower mosaic virus (CaMV were fused with β-glucuronidase (GUS and green fluorescent protein (GFP reporter genes. GUS activity in individual plant cells driven by Rep, CP and 35S promoters was estimated using real-time PCR and fluorometric GUS assay. Histochemical staining of GUS in transformed tobacco (Nicotiana tabacum cv. Xanthi leaves showed highest expression driven by Rep promoter followed by 35S promoter and CP promoter. The expression level of GUS driven by Rep promoter in transformed tobacco plants was shown to be two to four-fold higher than that of 35S promoter, while the expression by CP promoter was slightly lower. Further, the expression of GFP was monitored in agroinfiltrated leaves of N. benthamiana, N. tabacum and cotton (Gossypium hirsutum plants using confocal laser scanning microscopy. Rep promoter showed strong consistent transient expression in tobacco and cotton leaves as compared to 35S promoter. The strong constitutive CLCuBuV Rep promoter developed in this study could be very useful for high level expression of transgenes in a wide variety of plant cells.

  2. Functional Characterization of a Bidirectional Plant Promoter from Cotton Leaf Curl Burewala Virus Using an Agrobacterium-Mediated Transient Assay

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    Muhammad Aleem Ashraf

    2014-01-01

    Full Text Available The C1 promoter expressing the AC1 gene, and V1 promoter expressing the AV1 gene are located in opposite orientations in the large intergenic region of the Cotton leaf curl Burewala virus (CLCuBuV genome. Agro-infiltration was used to transiently express putative promoter constructs in Nicotiana tabacum and Gossypium hirsutum leaves, which was monitored by a GUS reporter gene, and revealed that the bidirectional promoter of CLCuBuV transcriptionally regulates both the AC1 and AV1 genes. The CLCuBuV C1 gene promoter showed a strong, consistent transient expression of the reporter gene (GUS in N. tabacum and G. hirsutum leaves and exhibited GUS activity two- to three-fold higher than the CaMV 35S promoter. The CLCuBuV bidirectional gene promoter is a nearly constitutive promoter that contains basic conserved elements. Many cis-regulatory elements (CREs were also analyzed within the bidirectional plant promoters of CLCuBuV and closely related geminiviruses, which may be helpful in understanding the transcriptional regulation of both the virus and host plant.

  3. Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato.

    Science.gov (United States)

    Yang, Xiaohui; Caro, Myluska; Hutton, Samuel F; Scott, John W; Guo, Yanmei; Wang, Xiaoxuan; Rashid, Md Harunur; Szinay, Dora; de Jong, Hans; Visser, Richard G F; Bai, Yuling; Du, Yongchen

    2014-01-01

    Resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (TYLCV), have been introgressed to cultivated tomato (Solanum lycopersicum) from wild tomato accessions. A major gene, Ty-2 from S. habrochaites f. glabratum accession "B6013," that confers resistance to TYLCV was previously mapped to a 19-cM region on the long arm of chromosome 11. In the present study, approximately 11,000 plants were screened and nearly 157 recombination events were identified between the flanking markers C2_At1g07960 (82.5 cM, physical distance 51.387 Mb) and T0302 (89 cM, 51.878 Mb). Molecular marker analysis of recombinants and TYLCV evaluation of progeny from these recombinants localized Ty-2 to an approximately 300,000-bp interval between markers UP8 (51.344 Mb) and M1 (51.645 Mb). No recombinants were identified between TG36 and C2_At3g52090, a region of at least 115 kb, indicating severe recombination suppression in this region. Due to the small interval, fluorescence in situ hybridization analysis failed to clarify whether recombination suppression is caused by chromosomal rearrangements. Candidate genes predicted based on tomato genome annotation were analyzed by RT-PCR and virus-induced gene silencing. Results indicate that the NBS gene family present in the Ty-2 region is likely not responsible for the Ty-2-conferred resistance and that two candidate genes might play a role in the Ty-2-conferred resistance. Several markers very tightly linked to the Ty-2 locus are presented and useful for marker-assisted selection in breeding programs to introgress Ty-2 for begomovirus resistance.

  4. Rapid spread of tomato yellow leaf curl virus in China is aided differentially by two invasive whiteflies.

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    Huipeng Pan

    Full Text Available BACKGROUND: Tomato yellow leaf curl virus (TYLCV was introduced into China in 2006, approximately 10 years after the introduction of an invasive whitefly, Bemisia tabaci (Genn. B biotype. Even so the distribution and prevalence of TYLCV remained limited, and the economic damage was minimal. Following the introduction of Q biotype into China in 2003, the prevalence and spread of TYLCV started to accelerate. This has lead to the hypothesis that the two biotypes might not be equally competent vectors of TYLCV. METHODOLOGY/PRINCIPAL FINDINGS: The infection frequency of TYLCV in the field-collected B. tabaci populations was investigated, the acquisition and transmission capability of TYLCV by B and Q biotypes were compared under the laboratory conditions. Analysis of B. tabaci populations from 55 field sites revealed the existence of 12 B and 43 Q biotypes across 18 provinces in China. The acquisition and transmission experiments showed that both B and Q biotypes can acquire and transmit the virus, however, Q biotype demonstrated superior acquisition and transmission capability than its B counterparts. Specifically, Q biotype acquired significantly more viral DNA than the B biotype, and reached the maximum viral load in a substantially shorter period of time. Although TYLCV was shown to be transmitted horizontally by both biotypes, Q biotype exhibited significantly higher viral transmission frequency than B biotype. Vertical transmission result, on the other hand, indicated that TYLCV DNA can be detected in eggs and nymphs, but not in pupae and adults of the first generation progeny. CONCLUSIONS/SIGNIFICANCE: These combined results suggested that the epidemiology of TYLCV was aided differentially by the two invasive whiteflies (B and Q biotypes through horizontal but not vertical transmission of the virus. This is consistent with the concomitant eruption of TYLCV in tomato fields following the recent rapid invasion of Q biotype whitefly in China.

  5. A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus

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    Bongjun Bang

    2014-09-01

    Full Text Available Tomato yellow leaf curl virus (TYLCV, a member of the genus Begomovirus, is responsible for one of the most devastating viral diseases in tomato-growing countries and is becoming a serious problem in many subtropical and tropical countries. The climate in Korea is getting warmer and developing subtropical features in response to global warming. These changes are being accompanied by TYLCV, which is now becoming a large problem in the Korean tomato industry. The most effective way to reduce damage caused by TYLCV is to breed resistant varieties of tomatoes. To accomplish this, it is necessary to establish a simple inoculation technique for the efficient evaluation of resistance to TYLCV. Here, we present the rolling circle amplification (RCA method, which employs a bacteriophage using phi-29 DNA polymerase for construction of infectious TYLCV clones. The RCA method is simple, does not require sequence information for cloning, and is less expensive and time consuming than conventional PCR based-methods. Furthermore, RCA-based construction of an infectious clone can be very useful to other emerging and unknown geminiviruses in Korea.

  6. A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus.

    Science.gov (United States)

    Bang, Bongjun; Lee, Jongyun; Kim, Sunyoung; Park, Jungwook; Nguyen, Thao Thi; Seo, Young-Su

    2014-09-01

    Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is responsible for one of the most devastating viral diseases in tomato-growing countries and is becoming a serious problem in many subtropical and tropical countries. The climate in Korea is getting warmer and developing subtropical features in response to global warming. These changes are being accompanied by TYLCV, which is now becoming a large problem in the Korean tomato industry. The most effective way to reduce damage caused by TYLCV is to breed resistant varieties of tomatoes. To accomplish this, it is necessary to establish a simple inoculation technique for the efficient evaluation of resistance to TYLCV. Here, we present the rolling circle amplification (RCA) method, which employs a bacteriophage using phi-29 DNA polymerase for construction of infectious TYLCV clones. The RCA method is simple, does not require sequence information for cloning, and is less expensive and time consuming than conventional PCR based-methods. Furthermore, RCA-based construction of an infectious clone can be very useful to other emerging and unknown geminiviruses in Korea.

  7. Expressing activity of promoter elements of large intergenic region from cotton leaf curl virus in host plant*

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Cotton leaf curl virus (CLCuV) is a type of single-stranded DNAvirus, belonging to geminivirus of subgroup III. In order to determine the function of CLCuV large intergenic region (LIR), total DNA of CLCuV-infected cotton leaves was used as template, and fragment of LIR was obtained by PCR and inserted into clone vector. The fragment of LIR was fused with gus reporter gene and nos terminator in the orientation of transcription of virion sense and complementary sense respectively, and the plant expression vectors were constructed. GUS activity of Agrobacterium-mediated transgenic tobacco was measured. The result indicated that LIR showed strong promoter activity in complementary sense gene orientation. Average GUS activity of the complementary sense promoter was 5-6 times that of CaMV 35S promoter, and the highest GUS activity of individual plant was ten times of that of CaMV 35S promoter. Histochemical localization confirmed its activity in both mesophyll and vascular tissues. Activity of virion sense of LIR was rather low. Thus LIR isolated from CLCuV could be used as a novel strong promoter in plant genetic manipulation.

  8. Correlation of meteorological parameters and remotely sensed normalized difference vegetation index (NDVI) with cotton leaf curl virus (CLCV) in Multan

    Science.gov (United States)

    Ahmed, A.; Akhtar, A.; Khalid, B.; Shamim, A.

    2013-06-01

    Climate change and weather has a profound effect on the spread of Cotton Leaf Curl Virus (CLCV) which is transmitted by whitefly. Climate change is altering temperature and precipitation patterns, resulting in the shift of some insect/pest from small population to large population thus effecting crops yield. To find out the relationship between the weather conditions, outburst of CLCV and changes in Normalized Difference Vegetation Index (NDVI) values due to the outburst of CLCV, a study was carried out for tehsil Multan. Data was acquired for the months of June, July, August and September for the year 2010. Regression analysis between CLCV and meteorological conditions as well as between CLCV and NDVI was performed. Meteorological parameters included temperature, humidity, precipitation, cloud cover, wind direction, pan evaporation and sunshine hours. NDVI values were calculated from SPOT satellite imagery (1km) using ArcMap10 and WinDisp v5.1. Correlation coefficients obtained in most of the cases were acceptable however the significance F and P-value were higher than their critical value at 95% level of significance. Therefore significant correlation was found only between CLCV and temperature and between CLCV and PAN evaporation during the month of July.

  9. Molecular characterization of sweet potato leaf curl virus isolate from China (SPLCV-CN) and its phylogenetic relationship with other members of the Geminiviridae.

    Science.gov (United States)

    Luan, Yu Shi; Zhang, Juan; Liu, Dan Mei; Li, Wen Li

    2007-10-01

    A Sweet potato-infecting sweet potato leaf curl virus (SPLCV) isolated in China was detected by Polymerase Chain Reaction (PCR). PCR products amplified from DNA-A were cloned and sequenced. The isolates of SPLCV from China(SPLCV-CN)has a genome organization similar to that of monopartite begomoviruses. The DNA-A had two ORFs (AV1 and AV2) in the virion sense and four ORFs (AC1, AC2, AC3, and AC4) in the complementary sense, separated by an intergenic region (IR) containing a conserved stem-loop motif. Three incomplete direct repeat iterons were also found within the IR. The presence of AV2 ORF supports the relationship of SPLCV-CN to the Old World gemimiviruses. Sequence comparisons showed that the DNA-A sequence of SPLCV-CN were closely related to those of sweet potato leaf curl Georgia virus-[16] (SPLCGV-[16]), Ipomoea yellow vein virus (IYVV-SI), and sweet potato leaf curl virus (SPLCV) with nucleotide sequence identity ranging from 88% to 91%. Comparison of individual encoded proteins between SPLCV-CN and that of three other SPLCV isolates showed the coat protein (AV1) shared the highest amino acid sequence identity (93%-96%), suggesting the coat protein of these viruses may have identical ancestor. The relationships between SPLCV-CN and other whitefly-transmitted geminiviruses were investigated by using phylogeny of derived AV1, AC1, and AV2 amino acid sequences. In all phylogenetic trees, SPLCV-CN clustered with three other isolates of SPLCV. The analyses revealed that the four isolates of SPLCV have coat proteins which are unique from its counterparts from both the Old World and New World. The present of AV2 and phylogenic analysis of AC1 suggest that SPLCV is more close to begomoviruses from the Old World but isolates of this virus seems to form a separate subset.

  10. Tomato yellow leaf curl virus resistance by Ty-1 involves increased cytosine methylation of viral genomes and is compromised by cucumber mosaic virus infection.

    Science.gov (United States)

    Butterbach, Patrick; Verlaan, Maarten G; Dullemans, Annette; Lohuis, Dick; Visser, Richard G F; Bai, Yuling; Kormelink, Richard

    2014-09-02

    Tomato yellow leaf curl virus (TYLCV) and related begomoviruses are a major threat to tomato production worldwide and, to protect against these viruses, resistance genes from different wild tomato species are introgressed. Recently, the Ty-1 resistance gene was identified, shown to code for an RNA-dependent RNA polymerase and to be allelic with Ty-3. Here we show that upon TYLCV challenging of resistant lines carrying Ty-1 or Ty-3, low virus titers were detected concomitant with the production of relatively high levels of siRNAs whereas, in contrast, susceptible tomato Moneymaker (MM) revealed higher virus titers but lower amounts of siRNAs. Comparative analysis of the spatial genomic siRNA distribution showed a consistent and subtle enrichment for siRNAs derived from the V1 and C3 genes in Ty-1 and Ty-3. In plants containing Ty-2 resistance the virus was hardly detectable, but the siRNA profile resembled the one observed in TYLCV-challenged susceptible tomato (MM). Furthermore, a relative hypermethylation of the TYLCV V1 promoter region was observed in genomic DNA collected from Ty-1 compared with that from (MM). The resistance conferred by Ty-1 was also effective against the bipartite tomato severe rugose begomovirus, where a similar genome hypermethylation of the V1 promoter region was discerned. However, a mixed infection of TYLCV with cucumber mosaic virus compromised the resistance. The results indicate that Ty-1 confers resistance to geminiviruses by increasing cytosine methylation of viral genomes, suggestive of enhanced transcriptional gene silencing. The mechanism of resistance and its durability toward geminiviruses under natural field conditions is discussed.

  11. A begomovirus associated with Ageratum yellow vein disease in Indonesia: evidence for natural recombination between tomato leaf curl Java virus and Ageratum yellow vein virus-[Java].

    Science.gov (United States)

    Kon, T; Kuwabara, K; Hidayat, S H; Ikegami, M

    2007-01-01

    A begomovirus (2747 nucleotides) and a satellite DNA beta component (1360 nucleotides) have been isolated from Ageratum conyzoides L. plants with yellow vein symptoms growing in Java, Indonesia. The begomovirus is most closely related to Tomato leaf curl Java virus (ToLCJV) (91 and 98% in the total nucleotide and coat protein amino acid sequences, respectively), although the products of ORFs C1 and C4 are more closely related to those of Ageratum yellow vein virus-[Java] (91 and 95% identity, respectively). For this reason, the begomovirus it is considered to be a strain of ToLCJV and is referred to as ToLCJV-Ageratum. The virus probably derives from a recombination event in which nucleotides 2389-2692 of ToLCJV have been replaced with the corresponding region of the AYVV-[Java] genome, which includes the 5' part of the intergenic region and the C1 and C4 ORFs. Infection of A. conyzoides with ToLCJV-Ageratum alone produced no symptoms, but co-infection with DNAbeta induced yellow vein symptoms. Symptoms induced in Nicotiana benthamiana by ToLCJV-Ageratum, ToLCJV and AYVV-[Java] are consistent with the exchange of pathogenicity determinant ORF C4 during recombination.

  12. Detection and molecular characterization of bovine leukemia virus in Philippine cattle.

    Science.gov (United States)

    Polat, Meripet; Ohno, Ayumu; Takeshima, Shin-Nosuke; Kim, Jiyun; Kikuya, Mari; Matsumoto, Yuki; Mingala, Claro Niegos; Onuma, Misao; Aida, Yoko

    2015-01-01

    Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. However, there are no comprehensive studies on the distribution of BLV in the Philippines, and the genetic characteristics of Philippine BLV strains are unknown. Therefore, the aim of this study was to detect BLV infections in the Philippines and determined their genetic variability. Blood samples were obtained from 1116 cattle from different farms on five Philippine islands, and BLV provirus was detected by BLV-CoCoMo-qPCR-2 and nested PCR targeting BLV long terminal repeats. Out of 1116 samples, 108 (9.7 %) and 54 (4.8 %) were positive for BLV provirus, as determined by BLV-CoCoMo-qPCR-2 and nested PCR, respectively. Of the five islands, Luzon Island showed the highest prevalence of BLV infection (23.1 %). Partial env gp51 genes from 43 samples, which were positive for BLV provirus by both methods, were sequenced for phylogenetic analysis. Phylogenetic analysis based on a 423-bp fragment of the env gene revealed that Philippine BLV strains clustered into either genotype 1 or genotype 6. Substitutions were mainly found in antigenic determinants, such as the CD4(+) T-cell epitope, the CD8(+) T-cell epitope, the second neutralizing domain, B and E epitopes, and these substitutions varied according to genotype. This study provides comprehensive information regarding BLV infection levels in the Philippines and documents the presence of two BLV genotypes, genotypes 1 and 6, in this population.

  13. Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus.

    Science.gov (United States)

    Seepiban, Channarong; Charoenvilaisiri, Saengsoon; Warin, Nuchnard; Bhunchoth, Anjana; Phironrit, Namthip; Phuangrat, Bencharong; Chatchawankanphanich, Orawan; Attathom, Supat; Gajanandana, Oraprapai

    2017-05-30

    Tomato yellow leaf curl Thailand virus, TYLCTHV, is a begomovirus that causes severe losses of tomato crops in Thailand as well as several countries in Southeast and East Asia. The development of monoclonal antibodies (MAbs) and serological methods for detecting TYLCTHV is essential for epidemiological studies and screening for virus-resistant cultivars. The recombinant coat protein (CP) of TYLCTHV was expressed in Escherichia coli and used to generate MAbs against TYLCTHV through hybridoma technology. The MAbs were characterized and optimized to develop triple antibody sandwich enzyme-linked immunosorbent assays (TAS-ELISAs) for begomovirus detection. The efficiency of TAS-ELISAs for begomovirus detection was evaluated with tomato, pepper, eggplant, okra and cucurbit plants collected from several provinces in Thailand. Molecular identification of begomoviruses in these samples was also performed through PCR and DNA sequence analysis of the CP gene. Two MAbs (M1 and D2) were generated and used to develop TAS-ELISAs for begomovirus detection. The results of begomovirus detection in 147 field samples indicated that MAb M1 reacted with 2 begomovirus species, TYLCTHV and Tobacco leaf curl Yunnan virus (TbLCYnV), whereas MAb D2 reacted with 4 begomovirus species, TYLCTHV, TbLCYnV, Tomato leaf curl New Delhi virus (ToLCNDV) and Squash leaf curl China virus (SLCCNV). Phylogenetic analyses of CP amino acid sequences from these begomoviruses revealed that the CP sequences of begomoviruses recognized by the narrow-spectrum MAb M1 were highly conserved, sharing 93% identity with each other but only 72-81% identity with MAb M1-negative begomoviruses. The CP sequences of begomoviruses recognized by the broad-spectrum MAb D2 demonstrated a wider range of amino acid sequence identity, sharing 78-96% identity with each other and 72-91% identity with those that were not detected by MAb D2. TAS-ELISAs using the narrow-specificity MAb M1 proved highly efficient for the detection of

  14. Identification and application of biocontrol agents against Cotton leaf curl virus disease in Gossypium hirsutum under greenhouse conditions

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    Memoona Ramzan

    2016-05-01

    Full Text Available Biological control is a novel approach in crop protection. Bacteria, such as Bacillus spp. and Pseudomonas spp., are reported for this purpose and some of their products are already commercially available. In this study, the rhizosphere and phyllosphere of healthy cotton plants were used as a source of bacterial isolates with properties of potential biocontrol agents. The isolates were screened for phosphate solubilization activity, indole acetic acid (IAA production and antifungal activity. Two isolates, S1HL3 and S1HL4, showed phosphate solubilization and IAA production simultaneously, while another two, JS2HR4 and JS3HR2, demonstrated potential to inhibit fungal pathogens. These bacteria were identified as Pseudomonas aeruginosa (S1HL3, Burkholderia sp. (S1HL4 and Bacillus sp. (JS2HR4 and JS3HR2 based on biochemical and molecular characteristics. The isolates were tested against Cotton leaf curl virus (CLCuV in greenhouse conditions, both as individual bacterial isolates and consortia. Treated plants were healthy as compared to control plants, where up to 74% of the plants were symptomatic for CLCuV infection. Maximum inhibition of CLCuV was observed in the plants treated with a mixture of bacterial isolates: the viral load in the treated plants was only 0.4% vs. up to 74% in controls. This treatment consortium included P. aeruginosa S1HL3, Burkholderia sp. S1HL4 and Bacillus spp. isolates, JS2HR4 and JS3HR2. The principal-component biplot showed a highly significant correlation between the viral load percentage and the disease incidence.

  15. The Tomato Yellow Leaf Curl Virus resistance genes Ty-1 and Ty-3 are allelic and code for DFDGD-class RNA-dependent RNA polymerases.

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    Maarten G Verlaan

    2013-03-01

    Full Text Available Tomato Yellow Leaf Curl Virus Disease incited by Tomato yellow leaf curl virus (TYLCV causes huge losses in tomato production worldwide and is caused by different related begomovirus species. Breeding for TYLCV resistance has been based on the introgression of multiple resistance genes originating from several wild tomato species. In this study we have fine-mapped the widely used Solanum chilense-derived Ty-1 and Ty-3 genes by screening nearly 12,000 plants for recombination events and generating recombinant inbred lines. Multiple molecular markers were developed and used in combination with disease tests to fine-map the genes to a small genomic region (approximately 70 kb. Using a Tobacco Rattle Virus-Virus Induced Gene Silencing approach, the resistance gene was identified. It is shown that Ty-1 and Ty-3 are allelic and that they code for a RNA-dependent RNA polymerase (RDR belonging to the RDRγ type, which has an atypical DFDGD motif in the catalytic domain. In contrast to the RDRα type, characterized by a catalytic DLDGD motif, no clear function has yet been described for the RDRγ type, and thus the Ty-1/Ty-3 gene unveils a completely new class of resistance gene. Although speculative, the resistance mechanism of Ty-1/Ty-3 and its specificity towards TYLCV are discussed in light of the function of the related RDRα class in the amplification of the RNAi response in plants and transcriptional silencing of geminiviruses in plants.

  16. Respiratory viruses from hospitalized children with severe pneumonia in the Philippines.

    Science.gov (United States)

    Suzuki, Akira; Lupisan, Socorro; Furuse, Yuki; Fuji, Naoko; Saito, Mariko; Tamaki, Raita; Galang, Hazel; Sombrero, Lydia; Mondoy, Melisa; Aniceto, Rapunzel; Olveda, Remigio; Oshitani, Hitoshi

    2012-10-23

    Pneumonia remains a leading cause of child death in developing countries. The viruses in severe pneumonia remain poorly defined. The study was conducted at the Eastern Visayas Regional Medical Center in Tacloban City, Philippines from May 2008 to May 2009. Patients aged 8 days to 13 years old who were admitted to the Department of Pediatrics with severe pneumonia were enrolled for the study. Upon admission, polymerase chain reaction was performed using nasopharyngeal swabs and blood cultures to detect respiratory viruses and bacteria, respectively. Among the 819 patients enrolled, at least one virus was detected in 501 cases (61.2%). In addition, 423 cases were positive for a single virus while bacteria were detected in the blood culture sample of 31 cases. The most commonly detected viruses were human rhinoviruses (n = 189), including types A (n = 103), B (n = 17), and C (n = 69), and respiratory syncytial virus (RSV) (n = 165). Novel viruses such as human metapneumovirus, human coronavirus NL63, human bocavirus, and human polyomaviruses WU and KI were also detected. There were 70 deaths, and one or more viruses were detected in 35 (50%) of these cases. Positivity only for influenza A virus (OR = 4.3, 95% CI = 1.3-14.6) was significantly associated with fatal outcome. From the blood culture, Burkholderia cepacia group (n = 9), Streptococcus pneumoniae (n = 4), Staphylococcus aureus (n = 4), Haemophilus influenzae (n = 1), and Salmonella C1 (n = 1) were also isolated. Viruses were commonly detected in children with severe pneumonia in the Philippines. Hence, viral etiologies should be considered while developing better effective strategies to reduce child pneumonia-related deaths in developing countries.

  17. Respiratory viruses from hospitalized children with severe pneumonia in the Philippines

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    Suzuki Akira

    2012-10-01

    Full Text Available Abstract Background Pneumonia remains a leading cause of child death in developing countries. The viruses in severe pneumonia remain poorly defined. Methods The study was conducted at the Eastern Visayas Regional Medical Center in Tacloban City, Philippines from May 2008 to May 2009. Patients aged 8 days to 13 years old who were admitted to the Department of Pediatrics with severe pneumonia were enrolled for the study. Upon admission, polymerase chain reaction was performed using nasopharyngeal swabs and blood cultures to detect respiratory viruses and bacteria, respectively. Result Among the 819 patients enrolled, at least one virus was detected in 501 cases (61.2%. In addition, 423 cases were positive for a single virus while bacteria were detected in the blood culture sample of 31 cases. The most commonly detected viruses were human rhinoviruses (n = 189, including types A (n = 103, B (n = 17, and C (n = 69, and respiratory syncytial virus (RSV (n = 165. Novel viruses such as human metapneumovirus, human coronavirus NL63, human bocavirus, and human polyomaviruses WU and KI were also detected. There were 70 deaths, and one or more viruses were detected in 35 (50% of these cases. Positivity only for influenza A virus (OR = 4.3, 95% CI = 1.3-14.6 was significantly associated with fatal outcome. From the blood culture, Burkholderia cepacia group (n = 9, Streptococcus pneumoniae (n = 4, Staphylococcus aureus (n = 4, Haemophilus influenzae (n = 1, and Salmonella C1 (n = 1 were also isolated. Conclusion Viruses were commonly detected in children with severe pneumonia in the Philippines. Hence, viral etiologies should be considered while developing better effective strategies to reduce child pneumonia-related deaths in developing countries.

  18. Local persistence and global dissemination play a significant role in the circulation of influenza B viruses in Leyte Island, Philippines.

    Science.gov (United States)

    Furuse, Yuki; Odagiri, Takashi; Tamaki, Raita; Kamigaki, Taro; Otomaru, Hirono; Opinion, Jamie; Santo, Arlene; Dolina-Lacaba, Donna; Daya, Edgard; Okamoto, Michiko; Saito-Obata, Mariko; Inobaya, Marianette; Tan, Alvin; Tallo, Veronica; Lupisan, Socorro; Suzuki, Akira; Oshitani, Hitoshi

    2016-05-01

    The local and global transmission dynamics of influenza B virus is not completely understood mainly because of limited epidemiological and sequence data for influenza B virus. Here we report epidemiological and molecular characteristics of influenza B viruses from 2010 to 2013 in Leyte Island, Philippines. Phylogenetic analyses showed global dissemination of the virus among both neighboring and distant areas. The analyses also suggest that southeast Asia is not a distributor of influenza B virus and can introduce the virus from other areas. Furthermore, we found evidence on the local persistence of the virus over years in the Philippines. Taken together, both local persistence and global dissemination play a significant role in the circulation of influenza B virus.

  19. Real-time PCR protocols for the quantification of the begomovirus tomato yellow leaf curl Sardinia virus in tomato plants and in its insect vector.

    Science.gov (United States)

    Noris, Emanuela; Miozzi, Laura

    2015-01-01

    Tomato yellow leaf curl Sardinia virus (TYLCSV) (Geminiviridae) is an important pathogen, transmitted by the whitefly Bemisia tabaci, that severely affects the tomato production in the Mediterranean basin. Here, we describe real-time PCR protocols suitable for relative and absolute quantification of TYLCSV in tomato plants and in whitefly extracts. Using primers and probe specifically designed for TYLCSV, the protocols for relative quantification allow to compare the amount of TYLCSV present in different plant or whitefly samples, normalized to the amount of DNA present in each sample using endogenous tomato or Bemisia genes as internal references. The absolute quantification protocol allows to calculate the number of genomic units of TYLCSV over the genomic units of the plant host (tomato), with a sensitivity of as few as ten viral genome copies per sample. The described protocols are potentially suitable for several applications, such as plant breeding for resistance, analysis of virus replication, and virus-vector interaction studies.

  20. Genetic characterization of measles virus in the Philippines, 2008-2011.

    Science.gov (United States)

    Centeno, Rex; Fuji, Naoko; Okamoto, Michiko; Dapat, Clyde; Saito, Mariko; Tandoc, Amado; Lupisan, Socorro; Oshitani, Hitoshi

    2015-06-03

    Large outbreaks of measles occurred in the Philippines in 2010 and 2011. Genetic analysis was performed to identify the genotype of measles virus (MeV) that was responsible for the large outbreaks. A total of 114 representative MeVs that were detected in the Philippines from 2008 to 2011 were analyzed by sequencing the C-terminal region of nucleocapsid (N) gene and partial hemagglutinin (H) gene and by inferring the phylogenetic trees. Genetic analysis showed that genotype D9 was the predominant circulating strain during the 4-year study period. Genotype D9 was detected in 23 samples (92%) by N gene sequencing and 93 samples (94%) by H gene analysis. Sporadic cases of genotype G3 MeV were identified in 2 samples (8%) by N gene sequencing and 6 samples (6%) by H gene analysis. Genotype G3 MeV was detected mainly in Panay Island in 2009 and 2010. Molecular clock analysis of N gene showed that the recent genotype D9 viruses that caused the big outbreaks in 2010 and 2011 diverged from a common ancestor in 2005 in one of the neighboring Southeast Asian countries, where D9 was endemic. These big outbreaks of measles resulted in a spillover and were associated with genotype D9 MeV importation to Japan and the USA. Genotype D9 MeV became endemic and caused two big outbreaks in the Philippines in 2010 and 2011. Genotype G3 MeV was detected sporadically with limited geographic distribution. This study highlights the importance of genetic analysis not only in helping with the assessment of measles elimination program in the country but also in elucidating the transmission dynamics of measles virus.

  1. Molecular Characterization of Human Respiratory Syncytial Virus in the Philippines, 2012-2013.

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    Rungnapa Malasao

    Full Text Available Human respiratory syncytial virus (HRSV is a major cause of acute lower respiratory tract infections in infants and children worldwide. We performed molecular analysis of HRSV among infants and children with clinical diagnosis of severe pneumonia in four study sites in the Philippines, including Biliran, Leyte, Palawan, and Metro Manila from June 2012 to July 2013. Nasopharyngeal swabs were collected and screened for HRSV using real-time polymerase chain reaction (PCR. Positive samples were tested by conventional PCR and sequenced for the second hypervariable region (2nd HVR of the G gene. Among a total of 1,505 samples, 423 samples were positive for HRSV (28.1%, of which 305 (72.1% and 118 (27.9% were identified as HRSV-A and HRSV-B, respectively. Two genotypes of HRSV-A, NA1 and ON1, were identified during the study period. The novel ON1 genotype with a 72-nucleotide duplication in 2nd HVR of the G gene increased rapidly and finally became the predominant genotype in 2013 with an evolutionary rate higher than the NA1 genotype. Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p<0.05 and massive O- and N-glycosylation in the 2nd HVR of the G gene. Among HRSV-B, BA9 was the predominant genotype circulating in the Philippines. However, two sporadic cases of GB2 genotype were found, which might share a common ancestor with other Asian strains. These findings suggest that HRSV is an important cause of severe acute respiratory infection among children in the Philippines and revealed the emergence and subsequent predominance of the ON1 genotype and the sporadic detection of the GB2 genotype. Both genotypes were detected for the first time in the Philippines.

  2. Molecular Characterization of Human Respiratory Syncytial Virus in the Philippines, 2012-2013

    Science.gov (United States)

    Malasao, Rungnapa; Okamoto, Michiko; Chaimongkol, Natthawan; Imamura, Tadatsugu; Tohma, Kentaro; Dapat, Isolde; Dapat, Clyde; Suzuki, Akira; Saito, Mayuko; Saito, Mariko; Tamaki, Raita; Pedrera-Rico, Gay Anne Granada; Aniceto, Rapunzel; Quicho, Reynaldo Frederick Negosa; Segubre-Mercado, Edelwisa; Lupisan, Socorro; Oshitani, Hitoshi

    2015-01-01

    Human respiratory syncytial virus (HRSV) is a major cause of acute lower respiratory tract infections in infants and children worldwide. We performed molecular analysis of HRSV among infants and children with clinical diagnosis of severe pneumonia in four study sites in the Philippines, including Biliran, Leyte, Palawan, and Metro Manila from June 2012 to July 2013. Nasopharyngeal swabs were collected and screened for HRSV using real-time polymerase chain reaction (PCR). Positive samples were tested by conventional PCR and sequenced for the second hypervariable region (2nd HVR) of the G gene. Among a total of 1,505 samples, 423 samples were positive for HRSV (28.1%), of which 305 (72.1%) and 118 (27.9%) were identified as HRSV-A and HRSV-B, respectively. Two genotypes of HRSV-A, NA1 and ON1, were identified during the study period. The novel ON1 genotype with a 72-nucleotide duplication in 2nd HVR of the G gene increased rapidly and finally became the predominant genotype in 2013 with an evolutionary rate higher than the NA1 genotype. Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p<0.05) and massive O- and N-glycosylation in the 2nd HVR of the G gene. Among HRSV-B, BA9 was the predominant genotype circulating in the Philippines. However, two sporadic cases of GB2 genotype were found, which might share a common ancestor with other Asian strains. These findings suggest that HRSV is an important cause of severe acute respiratory infection among children in the Philippines and revealed the emergence and subsequent predominance of the ON1 genotype and the sporadic detection of the GB2 genotype. Both genotypes were detected for the first time in the Philippines. PMID:26540236

  3. Tomato yellow leaf curl virus infection of tomato does not affect the performance of the Q and ZHJ2 biotypes of the viral vector Bemisia tabaci

    Institute of Scientific and Technical Information of China (English)

    Meng Li; Jian Liu; Shu-Sheng Liu

    2011-01-01

    To better understand the etiology of begomovirus epidemics in regions under invasion we need to know how indigenous and invasive whitefly vectors respond to virus infection.We investigated both direct and indirect effects of infection with Tomato yellow leaf curl virus(TYLCV)on the performance of the invasive Q biotype and the indigenous Asian ZHJ2 biotype of whitefly Bemisia tabaci.The Q biotype performed better than the ZHJ2 biotype on either uninfected or virus-infected tomato plants.However,virus-infection of host plants did not,or only marginally affected,the performance of either biotype of whiteflies m terms of fecundity,longevity,survival,development and population increase.Likewise,association of the vectors with TYLCV did not affect fecundity and longevity of the Q or ZHJ2 biotypes on cotton,a non-host of TYLCV.These results indicate that the alien Q biotype whitefly,but not the indigenous ZHJ2 biotype,is likely to become the major vector of TYLCV in the field and facilitate virus epidemics.

  4. Clathrin-mediated endocytosis is involved in Tomato yellow leaf curl virus transport across the midgut barrier of its whitefly vector.

    Science.gov (United States)

    Pan, Li-Long; Chen, Qun-Fang; Zhao, Juan-Juan; Guo, Tao; Wang, Xiao-Wei; Hariton-Shalev, Aliza; Czosnek, Henryk; Liu, Shu-Sheng

    2017-02-01

    Tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted by the whitefly Bemisia tabaci. The circulative translocation of the virus in the insect is known in its broad line. However, transit of TYLCV from the digestive tract into the haemolymph is poorly understood. We studied the involvement of clathrin in this process by disrupting the clathrin-mediated endocytosis and the endosome network using inhibitor feeding, antibody blocking and dsRNA silencing. We monitored the quantities of TYLCV in the whitefly and virus transmission efficiency. Following endocytosis and endosome network disruption, the quantity of virus was higher in the midgut relative to that of the whole insect body, and the quantity of virus in the haemolymph was reduced. The transmission efficiency of TYLCV by the treated insects was also reduced. These findings indicate that clathrin-mediated endocytosis and endosomes play an important role in the transport of TYLCV across the whitefly midgut. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  5. Response of resistant breeding lines of tomato germplasm and their progenies with Seedathip3 to Tomato Yellow Leaf Curl Virus, Thailand isolate (TYLCTHV-[2

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    Ornubol Chomdej

    2007-11-01

    Full Text Available Tomato germplasm accessions; FLA456-4, FLA591-15, H24, CLN2443A, CLN2443B, CLN2443C, TLB111, TLB182-1, TLB111-F6-4-1, TLB130-F6-3-1 and TLB134-F6-8-1 from the Asian Vegetable Research Development Center (AVRDC, Taiwan, were screened for resistance to the Tomato Yellow Leaf Curl Virus, Thailand isolate (TYLCTHV-[2]. The accessions expressing the resistant genotype were then crossed to the TYLCV-susceptible female parent, Seedathip3 (SD3, to produce F1 hybrids. Tomato parents and their F1 progenies were inoculated with TYLCTHV-[2] at 3 weeks of seedling age using viruliferous whitefly (Bemisia tabaci as the inoculation vector. Disease response of the seedling was rated according to the incidence and severity of the development of yellowing and curling symptoms. The presence of TYLCTHV-[2] in the inoculated plants was confirmed by Enzyme-Linked Immunosorbent Assay (ELISA. AVRDC tomato parental lines: H24, FLA591-15 and FLA456-4 expressed mild or no symptoms after one month inoculation. Progeny of crosses between the AVRDC donor parental lines and susceptible Thai cultivars showed intermediate tolerance to TYLCTHV-[2] infection. This indicated that resistance was incompletely dominant.

  6. Comparative transcriptome profiling of a resistant vs. susceptible tomato (Solanum lycopersicum cultivar in response to infection by tomato yellow leaf curl virus.

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    Tianzi Chen

    Full Text Available Tomato yellow leaf curl virus (TYLCV threatens tomato production worldwide by causing leaf yellowing, leaf curling, plant stunting and flower abscission. The current understanding of the host plant defense response to this virus is very limited. Using whole transcriptome sequencing, we analyzed the differential gene expression in response to TYLCV infection in the TYLCV-resistant tomato breeding line CLN2777A (R and TYLCV-susceptible tomato breeding line TMXA48-4-0 (S. The mixed inoculated samples from 3, 5 and 7 day post inoculation (dpi were compared to non-inoculated samples at 0 dpi. Of the total of 34831 mapped transcripts, 209 and 809 genes were differentially expressed in the R and S tomato line, respectively. The proportion of up-regulated differentially expressed genes (DEGs in the R tomato line (58.37% was higher than that in the S line (9.17%. Gene ontology (GO analyses revealed that similar GO terms existed in both DEGs of R and S lines; however, some sets of defense related genes and their expression levels were not similar between the two tomato lines. Genes encoding for WRKY transcriptional factors, R genes, protein kinases and receptor (-like kinases which were identified as down-regulated DEGs in the S line were up-regulated or not differentially expressed in the R line. The up-regulated DEGs in the R tomato line revealed the defense response of tomato to TYLCV infection was characterized by the induction and regulation of a series of genes involved in cell wall reorganization, transcriptional regulation, defense response, ubiquitination, metabolite synthesis and so on. The present study provides insights into various reactions underlining the successful establishment of resistance to TYLCV in the R tomato line, and helps in the identification of important defense-related genes in tomato for TYLCV disease management.

  7. Comparative Analyses of Tomato yellow leaf curl virus C4 Protein-Interacting Host Proteins in Healthy and Infected Tomato Tissues

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    Namgyu Kim

    2016-10-01

    Full Text Available Tomato yellow leaf curl virus (TYLCV, a member of the genus Begomovirus, is one of the most important viruses of cultivated tomatoes worldwide, mainly causing yellowing and curling of leaves with stunting in plants. TYLCV causes severe problems in sub-tropical and tropical countries, as well as in Korea. However, the mechanism of TYLCV infection remains unclear, although the function of each viral component has been identified. TYLCV C4 codes for a small protein involved in various cellular functions, including symptom determination, gene silencing, viral movement, and induction of the plant defense response. In this study, through yeast-two hybrid screenings, we identified TYLCV C4-interacting host proteins from both healthy and symptom-exhibiting tomato tissues, to determine the role of TYLCV C4 proteins in the infection processes. Comparative analyses of 28 proteins from healthy tissues and 36 from infected tissues showing interactions with TYLCV C4 indicated that TYLCV C4 mainly interacts with host proteins involved in translation, ubiquitination, and plant defense, and most interacting proteins differed between the two tissues but belong to similar molecular functional categories. Four proteins—two ribosomal proteins, S-adenosyl-L-homocysteine hydrolase, and 14-3-3 family protein—were detected in both tissues. Furthermore, the identified proteins in symptom-exhibiting tissues showed greater involvement in plant defenses. Some are key regulators, such as receptor-like kinases and pathogenesis-related proteins, of plant defenses. Thus, TYLCV C4 may contribute to the suppression of host defense during TYLCV infection and be involved in ubiquitination for viral infection.

  8. The complete genomic sequence of pepper yellow leaf curl virus (PYLCV and its implications for our understanding of evolution dynamics in the genus polerovirus.

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    Aviv Dombrovsky

    Full Text Available We determined the complete sequence and organization of the genome of a putative member of the genus Polerovirus tentatively named Pepper yellow leaf curl virus (PYLCV. PYLCV has a wider host range than Tobacco vein-distorting virus (TVDV and has a close serological relationship with Cucurbit aphid-borne yellows virus (CABYV (both poleroviruses. The extracted viral RNA was subjected to SOLiD next-generation sequence analysis and used as a template for reverse transcription synthesis, which was followed by PCR amplification. The ssRNA genome of PYLCV includes 6,028 nucleotides encoding six open reading frames (ORFs, which is typical of the genus Polerovirus. Comparisons of the deduced amino acid sequences of the PYLCV ORFs 2-4 and ORF5, indicate that there are high levels of similarity between these sequences to ORFs 2-4 of TVDV (84-93% and to ORF5 of CABYV (87%. Both PYLCV and Pepper vein yellowing virus (PeVYV contain sequences that point to a common ancestral polerovirus. The recombination breakpoint which is located at CABYV ORF3, which encodes the viral coat protein (CP, may explain the CABYV-like sequences found in the genomes of the pepper infecting viruses PYLCV and PeVYV. Two additional regions unique to PYLCV (PY1 and PY2 were identified between nucleotides 4,962 and 5,061 (ORF 5 and between positions 5,866 and 6,028 in the 3' NCR. Sequence analysis of the pepper-infecting PeVYV revealed three unique regions (Pe1-Pe3 with no similarity to other members of the genus Polerovirus. Genomic analyses of PYLCV and PeVYV suggest that the speciation of these viruses occurred through putative recombination event(s between poleroviruses co-infecting a common host(s, resulting in the emergence of PYLCV, a novel pathogen with a wider host range.

  9. Global Analysis of the Transcriptional Response of Whitefly to Tomato Yellow Leaf Curl China Virus Reveals the Relationship of Coevolved Adaptations▿ †

    Science.gov (United States)

    Luan, Jun-Bo; Li, Jun-Min; Varela, Nélia; Wang, Yong-Liang; Li, Fang-Fang; Bao, Yan-Yuan; Zhang, Chuan-Xi; Liu, Shu-Sheng; Wang, Xiao-Wei

    2011-01-01

    The begomoviruses are the largest and most economically important group of plant viruses transmitted exclusively by the whitefly Bemisia tabaci in a circulative, persistent manner. The circulation of the viruses within the insect vectors involves complex interactions between virus and vector components; however, the molecular mechanisms of these interactions remain largely unknown. Here we investigated the transcriptional response of the invasive B. tabaci Middle East-Asia Minor 1 species to Tomato yellow leaf curl China virus (TYLCCNV) using Illumina sequencing technology. Results showed that 1,606 genes involved in 157 biochemical pathways were differentially expressed in the viruliferous whiteflies. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that TYLCCNV can perturb the cell cycle and primary metabolism in the whitefly, which explains the negative effect of this virus on the longevity and fecundity of B. tabaci. Our data also demonstrated that TYLCCNV can activate whitefly immune responses, such as autophagy and antimicrobial peptide production, which might lead to a gradual decrease of viral particles within the body of the viruliferous whitefly. Furthermore, PCR results showed that TYLCCNV can invade the ovary and fat body tissues of the whitefly, and Lysotracker and Western blot analyses revealed that the invasion of TYLCCNV induced autophagy in both the ovary and fat body tissues. Surprisingly, TYLCCNV also suppressed the whitefly immune responses by downregulating the expression of genes involved in Toll-like signaling and mitogen-activated protein kinase (MAPK) pathways. Taken together, these results reveal the relationship of coevolved adaptations between begomoviruses and whiteflies and will provide a road map for future investigations into the complex interactions between plant viruses and their insect vectors. PMID:21270146

  10. Global analysis of the transcriptional response of whitefly to tomato yellow leaf curl China virus reveals the relationship of coevolved adaptations.

    Science.gov (United States)

    Luan, Jun-Bo; Li, Jun-Min; Varela, Nélia; Wang, Yong-Liang; Li, Fang-Fang; Bao, Yan-Yuan; Zhang, Chuan-Xi; Liu, Shu-Sheng; Wang, Xiao-Wei

    2011-04-01

    The begomoviruses are the largest and most economically important group of plant viruses transmitted exclusively by the whitefly Bemisia tabaci in a circulative, persistent manner. The circulation of the viruses within the insect vectors involves complex interactions between virus and vector components; however, the molecular mechanisms of these interactions remain largely unknown. Here we investigated the transcriptional response of the invasive B. tabaci Middle East-Asia Minor 1 species to Tomato yellow leaf curl China virus (TYLCCNV) using Illumina sequencing technology. Results showed that 1,606 genes involved in 157 biochemical pathways were differentially expressed in the viruliferous whiteflies. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that TYLCCNV can perturb the cell cycle and primary metabolism in the whitefly, which explains the negative effect of this virus on the longevity and fecundity of B. tabaci. Our data also demonstrated that TYLCCNV can activate whitefly immune responses, such as autophagy and antimicrobial peptide production, which might lead to a gradual decrease of viral particles within the body of the viruliferous whitefly. Furthermore, PCR results showed that TYLCCNV can invade the ovary and fat body tissues of the whitefly, and Lysotracker and Western blot analyses revealed that the invasion of TYLCCNV induced autophagy in both the ovary and fat body tissues. Surprisingly, TYLCCNV also suppressed the whitefly immune responses by downregulating the expression of genes involved in Toll-like signaling and mitogen-activated protein kinase (MAPK) pathways. Taken together, these results reveal the relationship of coevolved adaptations between begomoviruses and whiteflies and will provide a road map for future investigations into the complex interactions between plant viruses and their insect vectors.

  11. Development of primers for loop-mediated isothermal amplification of Philippine white spot syndrome virus isolates

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    Benedict A. Maralit

    2012-09-01

    Full Text Available Because there are currently no effective chemicals or drugs to completely treat bacterial andviral diseases, effective means of prevention such as early detection methods are continuously beingexplored and fully exhausted. Over the past few years, we have seen the development of cutting edgetechnologies to specifically and efficiently detect White Spot Syndrome Virus (WSSV, one of the majorviruses that devastated global shrimp aquaculture. The recent discovery of loop mediated isothermalamplification (LAMP brought about opportunities for fast diagnosis of a lot of animal diseases includinghumans. Here, the development of alternative LAMP primers specifically for Philippine WSSV isolates wasdiscussed. The sensitivity of the established detection protocol was found to be at 0.3954 pg of shrimpDNA template while exhibiting high specificity to the viral target. In addition, alternative visualizationtechniques and comparison with other detection protocols are also discussed.

  12. Whole-genome sequence analysis of Zika virus, amplified from urine of traveler from the Philippines.

    Science.gov (United States)

    Gu, Se Hun; Song, Dong Hyun; Lee, Daesang; Jang, Jeyoun; Kim, Min Young; Jung, Jaehun; Woo, Koung In; Kim, Mirang; Seog, Woong; Oh, Hong Sang; Choi, Byung Seop; Ahn, Jong-Seong; Park, Quehn; Jeong, Seong Tae

    2017-08-09

    Zika virus (ZIKV) (genus Flavivirus, family Flaviviridae) is an emerging pathogen associated with microcephaly and Guillain-Barré syndrome. The rapid spread of ZIKV disease in over 60 countries and the large numbers of travel-associated cases have caused worldwide concern. Thus, intensified surveillance of cases among immigrants and tourists from ZIKV-endemic areas is important for disease control and prevention. In this study, using Next Generation Sequencing, we reported the first whole-genome sequence of ZIKV strain AFMC-U, amplified from the urine of a traveler returning to Korea from the Philippines. Phylogenetic analysis showed geographic-specific clustering. Our results underscore the importance of examining urine in the diagnosis of ZIKV infection.

  13. Virion stability is important for the circulative transmission of tomato yellow leaf curl sardinia virus by Bemisia tabaci, but virion access to salivary glands does not guarantee transmissibility.

    Science.gov (United States)

    Caciagli, Piero; Medina Piles, Vicente; Marian, Daniele; Vecchiati, Manuela; Masenga, Vera; Mason, Giovanna; Falcioni, Tania; Noris, Emanuela

    2009-06-01

    The capsid protein (CP) of the monopartite begomovirus Tomato yellow leaf curl Sardinia virus (TYLCSV), family Geminiviridae, is indispensable for plant infection and vector transmission. A region between amino acids 129 and 152 is critical for virion assembly and insect transmissibility. Two previously described mutants, one with a double Q129P Q134H mutation (PNHD) and another with a further D152E change (PNHE), were found nontransmissible (NT). Another NT mutant with a single N130D change (QDQD) was retrieved from a new mutational analysis. In this study, these three NT mutants and the wild-type (wt) virus were compared in their relationships with the whitefly vector Bemisia tabaci and the nonvector Trialeurodes vaporariorum. Retention kinetics of NT mutants were analyzed by quantitative dot blot hybridization in whiteflies fed on infected plants. The QDQD mutant, whose virions appeared nongeminate following purification, was hardly detectable in either whitefly species at any sampling time. The PNHD mutant was acquired and circulated in both whitefly species for up to 10 days, like the wt virus, while PNHE circulated in B. tabaci only. Using immunogold labeling, both PNHD and PNHE CPs were detected in B. tabaci salivary glands (SGs) like the wt virus, while no labeling was found in any whitefly tissue with the QDQD mutant. Significant inhibition of transmission of the wt virus was observed after prior feeding of the insects on plants infected with the PNHE mutant, but not on plants infected with the other mutants. Virion stability and ability to cross the SG barrier are necessary for TYLCSV transmission, but interactions with molecular components inside the SGs are also critical for transmissibility.

  14. Discovering Host Genes Involved in the Infection by the Tomato Yellow Leaf Curl Virus Complex and in the Establishment of Resistance to the Virus Using Tobacco Rattle Virus-based Post Transcriptional Gene Silencing

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    Rosa Lozano-Durán

    2013-03-01

    Full Text Available The development of high-throughput technologies allows for evaluating gene expression at the whole-genome level. Together with proteomic and metabolomic studies, these analyses have resulted in the identification of plant genes whose function or expression is altered as a consequence of pathogen attacks. Members of the Tomato yellow leaf curl virus (TYLCV complex are among the most important pathogens impairing production of agricultural crops worldwide. To understand how these geminiviruses subjugate plant defenses, and to devise counter-measures, it is essential to identify the host genes affected by infection and to determine their role in susceptible and resistant plants. We have used a reverse genetics approach based on Tobacco rattle virus-induced gene silencing (TRV-VIGS to uncover genes involved in viral infection of susceptible plants, and to identify genes underlying virus resistance. To identify host genes with a role in geminivirus infection, we have engineered a Nicotiana benthamiana line, coined 2IRGFP, which over-expresses GFP upon virus infection. With this system, we have achieved an accurate description of the dynamics of virus replication in space and time. Upon silencing selected N. benthamiana genes previously shown to be related to host response to geminivirus infection, we have identified eighteen genes involved in a wide array of cellular processes. Plant genes involved in geminivirus resistance were studied by comparing two tomato lines: one resistant (R, the other susceptible (S to the virus. Sixty-nine genes preferentially expressed in R tomatoes were identified by screening cDNA libraries from infected and uninfected R and S genotypes. Out of the 25 genes studied so far, the silencing of five led to the total collapse of resistance, suggesting their involvement in the resistance gene network. This review of our results indicates that TRV-VIGS is an exquisite reverse genetics tool that may provide new insights into the

  15. The Whitefly Bemisia tabaci Knottin-1 Gene Is Implicated in Regulating the Quantity of Tomato Yellow Leaf Curl Virus Ingested and Transmitted by the Insect

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    Aliza Hariton Shalev

    2016-07-01

    Full Text Available The whitefly Bemisia tabaci is a major pest to agricultural crops. It transmits begomoviruses, such as Tomato yellow leaf curl virus (TYLCV, in a circular, persistent fashion. Transcriptome analyses revealed that B. tabaci knottin genes were responsive to various stresses. Upon ingestion of tomato begomoviruses, two of the four knottin genes were upregulated, knot-1 (with the highest expression and knot-3. In this study, we examined the involvement of B. tabaci knottin genes in relation to TYLCV circulative transmission. Knottins were silenced by feeding whiteflies with knottin dsRNA via detached tomato leaves. Large amounts of knot-1 transcripts were present in the abdomen of whiteflies, an obligatory transit site of begomoviruses in their circulative transmission pathway; knot-1 silencing significantly depleted the abdomen from knot-1 transcripts. Knot-1 silencing led to an increase in the amounts of TYLCV ingested by the insects and transmitted to tomato test plants by several orders of magnitude. This effect was not observed following knot-3 silencing. Hence, knot-1 plays a role in restricting the quantity of virions an insect may acquire and transmit. We suggest that knot-1 protects B. tabaci against deleterious effects caused by TYLCV by limiting the amount of virus associated with the whitefly vector.

  16. Genome-wide analysis of bHLH transcription factor and involvement in the infection by yellow leaf curl virus in tomato (Solanum lycopersicum).

    Science.gov (United States)

    Wang, Jinyan; Hu, Zhongze; Zhao, Tongmin; Yang, Yuwen; Chen, Tianzi; Yang, Mali; Yu, Wengui; Zhang, Baolong

    2015-02-05

    The basic helix-loop-helix (bHLH) proteins are a superfamily of transcription factors that can bind to specific DNA target sites. They have been well characterized in model plants such as Arabidopsis and rice and have been shown to be important regulatory components in many different biological processes. However, no systemic analysis of the bHLH transcription factor family has yet been reported in tomatoes. Tomato yellow leaf curl virus (TYLCV) threatens tomato production worldwide by causing leaf yellowing, leaf curling, plant stunting and flower abscission. A total of 152 bHLH transcription factors were identified from the entire tomato genome. Phylogenetic analysis of bHLH domain sequences from Arabidopsis and tomato facilitated classification of these genes into 26 subfamilies. The evolutionary and possible functional relationships revealed during this analysis are supported by other criteria, including the chromosomal distribution of these genes, the conservation of motifs and exon/intron structural patterns, and the predicted DNA binding activities within subfamilies. Distribution mapping results showed bHLH genes were localized on the 12 tomato chromosomes. Among the 152 bHLH genes from the tomato genome, 96 bHLH genes were detected in the TYLCV-susceptible and resistant tomato breeding line before (0 dpi) and after TYLCV (357 dpi) infection. As anticipated, gene ontology (GO) analysis indicated that most bHLH genes are related to the regulation of macromolecule metabolic processes and gene expression. Only four bHLH genes were differentially expressed between 0 and 357 dpi. Virus-induced gene silencing (VIGS) of one bHLH genes SlybHLH131 in resistant lines can lead to the cell death. In the present study, 152 bHLH transcription factor genes were identified. One of which bHLH genes, SlybHLH131, was found to be involved in the TYLCV infection through qRT-PCR expression analysis and VIGS validation. The isolation and identification of these bHLH transcription

  17. 番茄黄化曲叶病毒病暴发原因分析及防控对策%Analysis on the outbreaks of tomato yellow leaf curl virus and its control strategy

    Institute of Scientific and Technical Information of China (English)

    尹哲; 杨普云; 刘树生; 赵中华; 单绪南

    2010-01-01

    @@ 番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)为双生病毒科(Geminniviridae)菜豆金色花叶病毒属(Begomovirus)成员[1].该类病毒通过B型烟粉虱和Q型烟粉虱进行传播.同时可经嫁接传播,但不能经机械摩擦或种子传播[2].

  18. Prediction and characterization of Tomato leaf curl New Delhi virus (ToLCNDV) responsive novel microRNAs in Solanum lycopersicum.

    Science.gov (United States)

    Pradhan, Bhubaneswar; Naqvi, Afsar Raza; Saraf, Shradha; Mukherjee, Sunil Kumar; Dey, Nrisingha

    2015-01-02

    Tomato leaf curl New Delhi virus (ToLCNDV) infects tomato (Solanum lycopersicum) plants and causes severe crop losses. As the microRNAs (miRNAs) are deregulated during stressful events, such as biotic stress, we wanted to study the effect of ToLCNDV infection on tomato miRNAs. We constructed two libraries, isolating small RNAs (sRNAs) from healthy (HT) and ToLCNDV infected (IT) tomato leaves, and sequenced the library-specific sRNAs using the next generation sequencing (NGS) approach. These data helped predict 112 mature miRNA sequences employing the miRDeep-P program. A substantial number (58) of the sequences were 24-mer in size, which was a bit surprising. Based on the calculation of precision values, 53 novel miRNAs were screened from the predicted sequences. Nineteen of these were chosen for expression analysis; a northern blot analysis showed 15 to be positive. Many of the predicted miRNAs were up-regulated following viral infection. The target genes of the miRNAs were also predicted and the expression analysis of selected transcripts showed a typical inverse relation between the accumulation of target transcripts and the abundance of corresponding miRNAs. Furthermore, the cleavage sites of the target transcripts for three novel miRNAs were mapped, confirming the correct annotation of the miRNA-targets. The sRNA deep sequencing clearly revealed that the virus modulated global miRNA expression in the host. The validated miRNAs (Tom_4; Tom_14; Tom_17; Tom_21; Tom_29; Tom_43) could be valuable tools for understanding the ToLCNDV-tomato interaction, ultimately leading to the development of a virus-resistant tomato plant. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Cestrum yellow leaf curling virus (CmYLCV) promoter: a new strong constitutive promoter for heterologous gene expression in a wide variety of crops.

    Science.gov (United States)

    Stavolone, Livia; Kononova, Maria; Pauli, Sandra; Ragozzino, Antonio; de Haan, Peter; Milligan, Steve; Lawton, Kay; Hohn, Thomas

    2003-11-01

    Appropriately regulated gene expression requires a suitable promoter. A number of promoters have been isolated and shown to be functional in plants, but only a few of them activate transcription of transgenes at high levels constitutively. We report here the cloning and characterization of a novel, constitutively expressed promoter isolated from Cestrum yellow leaf curling virus (CmYLCV), a double-stranded DNA plant pararetrovirus belonging to the Caulimoviridae family. The CmYLCV promoter is highly active in callus, meristems and vegetative and reproductive tissues in Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Zea mays and Oryza sativa. Furthermore, the level of expression is comparable to, or higher than, that from the CaMV 35S, the 'super-promoter' or the maize ubiquitin 1 promoters, three frequently used promoters in agricultural biotechnology. The heritable, strong and constitutive activity in both monocotyledonous and dicotyledonous plants, combined with the extremely narrow CmYLCV host range, makes the CmYLCV promoter an attractive tool for regulating transgene expression in a wide variety of plant species.

  20. Sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation.

    Science.gov (United States)

    Kil, Eui-Joon; Byun, Hee-Seong; Kim, Sunhoo; Kim, Jaedeok; Park, Jungan; Cho, Seungchan; Yang, Dong-Cheol; Lee, Kyeong-Yeoll; Choi, Hong-Soo; Kim, Ji-Kwang; Lee, Sukchan

    2014-09-01

    Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, has a single-stranded DNA genome. TYLCV can induce severe disease symptoms on tomato plants, but other hosts plants such as cucurbits and peppers are asymptomatic. A full-length DNA clone of a Korean TYLCV isolate was constructed by rolling-circle amplification from TYLCV-infected tomatoes in Korea. To assess relative susceptibility of sweet pepper varieties to TYLCV, 19 cultivars were inoculated with cloned TYLCV by agro-inoculation. All TYLCV-infected sweet peppers were asymptomatic, even though Southern hybridization and polymerase chain reaction analysis showed TYLCV genomic DNA accumulation in roots, stems, and newly produced shoots. Southern hybridization indicated that TYLCV replicated and moved systemically from agro-inoculated apical shoot tips to roots or newly produced shoots of sweet peppers. Whitefly-mediated inoculation experiments showed that TYLCV can be transmitted to tomatoes from TYLCV-infected sweet peppers. Taken together, these results indicate that sweet pepper can be a reservoir for TYLCV in nature.

  1. Eugenol confers resistance to Tomato yellow leaf curl virus (TYLCV) by regulating the expression of SlPer1 in tomato plants.

    Science.gov (United States)

    Sun, Wei-Jie; Lv, Wen-Jing; Li, Li-Na; Yin, Gan; Hang, Xiaofang; Xue, Yanfeng; Chen, Jian; Shi, Zhiqi

    2016-05-25

    Tomato yellow leaf curl virus (TYLCV) is one of the most devastating plant diseases, and poses a significant agricultural concern because of the lack of an efficient control method. Eugenol is a plant-derived natural compound that has been widely used as a food additive and in medicine. In the present study, we demonstrated the potential of eugenol to enhance the resistance of tomato plants to TYLCV. The anti-TYLCV efficiency of eugenol was significantly higher than that of moroxydine hydrochloride (MH), a widely used commercial antiviral agent. Eugenol application stimulated the production of endogenous nitric oxide (NO) and salicylic acid (SA) in tomato plants. The full-length cDNA of SlPer1, which has been suggested to be a host R gene specific to TYLCV, was isolated from tomato plants. A sequence analysis suggested that SlPer1 might be a nucleobase-ascorbate transporter (NAT) belonging to the permease family. The transcript levels of SlPer1 increased markedly in response to treatment with eugenol or TYLCV inoculation. The results of this study also showed that SlPer1 expression was strongly induced by SA, MeJA (jasmonic acid methyl ester), and NO. Thus, we propose that the increased transcription of SlPer1 contributed to the high anti-TYLCV efficiency of eugenol, which might involve in the generation of endogenous SA and NO. Such findings provide the basis for the development of eugenol as an environmental-friendly agricultural antiviral agent.

  2. Whitefly population dynamics and evaluation of whitefly-transmitted tomato yellow leaf curl virus (TYLCV)-resistant tomato genotypes as whitefly and TYLCV reservoirs.

    Science.gov (United States)

    Srinivasan, Rajagopalbabu; Riley, David; Diffie, Stan; Sparks, Alton; Adkins, Scott

    2012-08-01

    Sweetpotato whitefly, Bemisia tabaci (Gennadius), and whitefly-transmitted tomato yellow leaf curl virus (TYLCV) are major threats to tomato production in the southeastern United States. TYLCV was introduced to Florida from the Caribbean islands and has spread to other southern states of the United States. In Georgia, in recent years, the incidence of TYLCV has been steadily increasing. Studies were conducted to monitor population dynamics of whiteflies in the vegetable production belt of Georgia, to evaluate TYLCV-resistant genotypes against whiteflies and TYLCV, and to assess the potential role of resistant genotypes in TYLCV epidemiology. Monitoring studies indicated that the peak incidence of whiteflies varied seasonally from year to year. In general, whitefly populations were not uniformly distributed. Tomato genotypes exhibited minor differences in their ability to support whitefly populations. TYLCV symptoms were visually undetectable in all but one resistant genotype. The infection rates (visually) in susceptible genotypes ranged from 40 to 87%. Greenhouse inoculations with viruliferous whiteflies followed by polymerase chain reaction (PCR) indicated that up to 100% of plants of resistant genotypes were infected, although predominantly symptomless. TYLCV acquisition by whiteflies from TYLCV-infected genotypes was tested by PCR; TYLCV acquisition rates from resistant genotypes were less than from susceptible genotypes. Nevertheless, this difference did not influence TYLCV transmission rates from resistant to susceptible genotypes. Results emphasize that resistant genotypes can serve as TYLCV and whitefly reservoirs and potentially influence TYLCV epidemics.

  3. Effectiveness of Cyantraniliprole for Managing Bemisia tabaci (Hemiptera: Aleyrodidae) and Interfering with Transmission of Tomato Yellow Leaf Curl Virus on Tomato.

    Science.gov (United States)

    Caballero, Rafael; Schuster, David J; Peres, Natalia A; Mangandi, Jozer; Hasing, Tomas; Trexler, Fred; Kalb, Steve; Portillo, Héctor E; Marçon, Paula C; Annan, I B

    2015-06-01

    Cyantraniliprole is the second xylem-systemic active ingredient in the new anthranilic diamide class. Greenhouse (2006), growth chamber (2007), and field studies (2009-2010) were conducted to determine the efficacy of cyantraniliprole for managing Bemisia tabaci (Gennadius) biotype B and in interfering with transmission of tomato yellow leaf curl virus (TYLCV) by this whitefly. Cyantraniliprole applied as soil treatments (200 SC) or foliar sprays (100 OD) provided excellent adult whitefly control, TYLCV suppression, and reduced oviposition and nymph survival, comparable to current standards. The positive results observed in these greenhouse experiments with a high level of insect pressure (10× the field threshold of one adult per plant) and disease pressure (five adults per plant, with a high level of confidence that TYLCV virulent adults were used), indicate a great potential for cyantraniliprole to be used in a whitefly management program. Field evaluations of soil drench treatments confirmed the suppression of TYLCV transmission demonstrated in the greenhouse studies. Field studies in 2009 and 2010 showed that cyantraniliprole (200 SC) provided TYLCV suppression for 2 wk after a drench application, when using a susceptible (2009) or imidacloprid-tolerant (2010) whitefly population. Cyantraniliprole was demonstrated to be a promising tool for management of TYLCV in tomato production, which is very difficult and expensive, and which has limited options. The integration of cyantraniliprole into a resistance management program will help to ensure the continued sustainability of this and current insecticides used for the management of insect vectors, including whiteflies and the TYLCV they spreads.

  4. Development of Gene Expression and Gene Silencing Vectors Based onCotton Leaf Curl Virus for Functional Genomics in Cotton `%基于抗卷叶病毒棉花基因组研究的基因表达和基因沉默载体的构建

    Institute of Scientific and Technical Information of China (English)

    Shahid MANSOOR; Muhammad SAQIB; Mazhar HUSSAIN; Rob BRIDDON; Kauser A. MALIK; Yusuf ZAFAR

    2002-01-01

    @@ Cotton leaf curl virus is the first example of a cotton-infecting virus where infectious clones are available. Plant viruses are valuable tools in understanding plant biology as they can be engineered for expression of foreign genes or silencing of genes homologous to cloned genes.

  5. SlMAPK3 enhances tolerance to tomato yellow leaf curl virus (TYLCV) by regulating salicylic acid and jasmonic acid signaling in tomato (Solanum lycopersicum).

    Science.gov (United States)

    Li, Yunzhou; Qin, Lei; Zhao, Jingjing; Muhammad, Tayeb; Cao, Hehe; Li, Hailiang; Zhang, Yan; Liang, Yan

    2017-01-01

    Several recent studies have reported on the role of mitogen-activated protein kinase (MAPK3) in plant immune responses. However, little is known about how MAPK3 functions in tomato (Solanum lycopersicum L.) infected with tomato yellow leaf curl virus (TYLCV). There is also uncertainty about the connection between plant MAPK3 and the salicylic acid (SA) and jasmonic acid (JA) defense-signaling pathways. The results of this study indicated that SlMAPK3 participates in the antiviral response against TYLCV. Tomato seedlings were inoculated with TYLCV to investigate the possible roles of SlMAPK1, SlMAPK2, and SlMAPK3 against this virus. Inoculation with TYLCV strongly induced the expression and the activity of all three genes. Silencing of SlMAPK1, SlMAPK2, and SlMAPK3 reduced tolerance to TYLCV, increased leaf H2O2 concentrations, and attenuated expression of defense-related genes after TYLCV infection, especially in SlMAPK3-silenced plants. Exogenous SA and methyl jasmonic acid (MeJA) both significantly induced SlMAPK3 expression in tomato leaves. Over-expression of SlMAPK3 increased the transcript levels of SA/JA-mediated defense-related genes (PR1, PR1b/SlLapA, SlPI-I, and SlPI-II) and enhanced tolerance to TYLCV. After TYLCV inoculation, the leaves of SlMAPK3 over-expressed plants compared with wild type plants showed less H2O2 accumulation and greater superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) activity. Overall, the results suggested that SlMAPK3 participates in the antiviral response of tomato to TYLCV, and that this process may be through either the SA or JA defense-signaling pathways.

  6. SlMAPK3 enhances tolerance to tomato yellow leaf curl virus (TYLCV) by regulating salicylic acid and jasmonic acid signaling in tomato (Solanum lycopersicum)

    Science.gov (United States)

    Li, Yunzhou; Qin, Lei; Zhao, Jingjing; Muhammad, Tayeb; Cao, Hehe; Li, Hailiang; Zhang, Yan; Liang, Yan

    2017-01-01

    Several recent studies have reported on the role of mitogen-activated protein kinase (MAPK3) in plant immune responses. However, little is known about how MAPK3 functions in tomato (Solanum lycopersicum L.) infected with tomato yellow leaf curl virus (TYLCV). There is also uncertainty about the connection between plant MAPK3 and the salicylic acid (SA) and jasmonic acid (JA) defense-signaling pathways. The results of this study indicated that SlMAPK3 participates in the antiviral response against TYLCV. Tomato seedlings were inoculated with TYLCV to investigate the possible roles of SlMAPK1, SlMAPK2, and SlMAPK3 against this virus. Inoculation with TYLCV strongly induced the expression and the activity of all three genes. Silencing of SlMAPK1, SlMAPK2, and SlMAPK3 reduced tolerance to TYLCV, increased leaf H2O2 concentrations, and attenuated expression of defense-related genes after TYLCV infection, especially in SlMAPK3-silenced plants. Exogenous SA and methyl jasmonic acid (MeJA) both significantly induced SlMAPK3 expression in tomato leaves. Over-expression of SlMAPK3 increased the transcript levels of SA/JA-mediated defense-related genes (PR1, PR1b/SlLapA, SlPI-I, and SlPI-II) and enhanced tolerance to TYLCV. After TYLCV inoculation, the leaves of SlMAPK3 over-expressed plants compared with wild type plants showed less H2O2 accumulation and greater superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) activity. Overall, the results suggested that SlMAPK3 participates in the antiviral response of tomato to TYLCV, and that this process may be through either the SA or JA defense-signaling pathways. PMID:28222174

  7. Natural vertical transmission of dengue viruses in Aedes aegypti in selected sites in Cebu City, Philippines.

    Science.gov (United States)

    Edillo, Frances E; Sarcos, Janet R; Sayson, Stephanie L

    2015-12-01

    We attempted to determine the vertical transmission of dengue virus (DENV) in Aedes aegypti in selected sites in Cebu City, Philippines. Mosquito sub-adults were collected monthly from households and the field during the wet-dry-wet season from November, 2011 to July, 2012 and were laboratory-reared to adults. Viral RNA extracts in mosquitoes were assayed by hemi-nested RT-PCR. Results showed that 62 (36.26%; n=679) out of 171 mosquito pools (n=2,871) were DENV+. The minimum infection rate (MIR) of DENV ranged from 0 in wet months to 48.22/1,000 mosquitoes in April, 2012 (mid-dry). DENVs were detected in larvae, pupae, and male and female adults, with DENV-4, DENV-3, and DENV-1, in that rank of prevalence. DENV-1 co-infected with either DENV-3 or -4 or with both in April, 2012; DENV-3 and -4 were present in both seasons. More DENV+ mosquitoes were collected from households than in field premises (p0.05). By Generalized Linear Mixed models, the type of premises nested in sites and monthly total rainfall were significant predictors of monthly dengue cases (pdengue outbreak.

  8. 棉花曲叶病毒对棉花造成的经济损失评估%The economic losses of Cottone leaf curl virus invading China on cotton

    Institute of Scientific and Technical Information of China (English)

    饶玉燕; 沈玲丽; 朱水芳; 严进; 黄冠胜

    2011-01-01

    棉花曲叶病毒(Cotton leaf curl virus)是我国进境检疫性病毒,严重危害棉花的生长,给棉花生产带来巨大经济损失.文中分析此病毒可能给棉花生产所造成的损失,包括直接经济损失、间接经济损失和防治费用.估算了棉花曲叶病毒对棉花造成的经济损失值为77.27亿元~498.61亿元.%The Cotton leaf curl virus as a quarantine virus in China severly harmed cotton, and leaded to seriously economical losing. In this paper we analyzed all the possible losses including three parts: direct economic losses,indirect economic losses and the cost of its control on this virus, and estimated the losses would be about 7727 million to 49 861 million yuan RMB.

  9. MicroRNA profiling of the whitefly Bemisia tabaci Middle East-Aisa Minor I following the acquisition of Tomato yellow leaf curl China virus.

    Science.gov (United States)

    Wang, Bi; Wang, Lanlan; Chen, Fangyuan; Yang, Xiuling; Ding, Ming; Zhang, Zhongkai; Liu, Shu-Sheng; Wang, Xiao-Wei; Zhou, Xueping

    2016-02-02

    The begomoviruses are the largest and most economically important group of plant viruses exclusively vectored by whitefly (Bemisia tabaci) in a circulative, persistent manner. During this process, begomoviruses and whitefly vectors have developed close relationships and complex interactions. However, the molecular mechanisms underlying these interactions remain largely unknown, and the microRNA profiles for viruliferous and nonviruliferous whiteflies have not been studied. Sequences of Argonaute 1(Ago1) and Dicer 1 (Dcr1) genes were cloned from B. tabaci MEAM1 cDNAs. Subsequently, deep sequencing of small RNA libraries from uninfected and Tomato yellow leaf curl China virus (TYLCCNV)-infected whiteflies was performed. The conserved and novel miRNAs were identified using the release of miRBase Version 19.0 and the prediction software miRDeep2, respectively. The sequencing results of selected deregulated and novel miRNAs were further confirmed using quantitative reverse transcription-PCR. Moreover, the previously published B. tabaci MEAM1 transcriptome database and the miRNA target prediction algorithm miRanda 3.1 were utilized to predict potential targets for miRNAs. Gene Ontology (GO) analysis was also used to classify the potential enriched functional groups of their putative targets. Ago1 and Dcr1orthologs with conserved domains were identified from B. tabaci MEAM1. BLASTn searches and sequence analysis identified 112 and 136 conserved miRNAs from nonviruliferous and viruliferous whitefly libraries respectively, and a comparison of the conserved miRNAs of viruliferous and nonviruliferous whiteflies revealed 15 up- and 9 down-regulated conserved miRNAs. 7 novel miRNA candidates with secondary pre-miRNA hairpin structures were also identified. Potential targets of conserved and novel miRNAs were predicted using GO analysis, for the targets of up- and down-regulated miRNAs, eight and nine GO terms were significantly enriched. We identified Ago1 and Dcr1 orthologs

  10. Influenza and other respiratory viruses detected by influenza-like illness surveillance in Leyte Island, the Philippines, 2010-2013.

    Directory of Open Access Journals (Sweden)

    Hirono Otomaru

    Full Text Available This study aimed to determine the role of influenza-like illness (ILI surveillance conducted on Leyte Island, the Philippines, including involvement of other respiratory viruses, from 2010 to 2013. ILI surveillance was conducted from January 2010 to March 2013 with 3 sentinel sites located in Tacloban city, Palo and Tanauan of Leyte Island. ILI was defined as fever ≥38°C or feverish feeling and either cough or running nose in a patient of any age. Influenza virus and other 5 respiratory viruses were searched. A total of 5,550 ILI cases visited the 3 sites and specimens were collected from 2,031 (36.6% cases. Among the cases sampled, 1,637 (75.6% were children aged <5 years. 874 (43.0% cases were positive for at least one of the respiratory viruses tested. Influenza virus and respiratory syncytial virus (RSV were predominantly detected (both were 25.7% followed by human rhinovirus (HRV (17.5%. The age distributions were significantly different between those who were positive for influenza, HRV, and RSV. ILI cases were reported throughout the year and influenza virus was co-detected with those viruses on approximately half of the weeks of study period (RSV in 60.5% and HRV 47.4%. In terms of clinical manifestations, only the rates of headache and sore throat were significantly higher in influenza positive cases than cases positive to other viruses. In conclusion, syndromic ILI surveillance in this area is difficult to detect the start of influenza epidemic without laboratory confirmation which requires huge resources. Age was an important factor that affected positive rates of influenza and other respiratory viruses. Involvement of older age children may be useful to detect influenza more effectively.

  11. Whitefly resistance traits derived from the wild tomato Solanum pimpinellifolium affect the preference and feeding behavior of Bemisia tabaci and reduce the spread of Tomato yellow leaf curl virus.

    Science.gov (United States)

    Rodríguez-López, M J; Garzo, E; Bonani, J P; Fereres, A; Fernández-Muñoz, R; Moriones, E

    2011-10-01

    Breeding of tomato genotypes that limit whitefly (Bemisia tabaci) access and feeding might reduce the spread of Tomato yellow leaf curl virus (TYLCV), a begomovirus (genus Begomovirus, family Geminiviridae) that is the causal agent of tomato yellow leaf curl disease. TYLCV is restricted to the phloem and is transmitted in a persistent manner by B. tabaci. The tomato breeding line ABL 14-8 was developed by introgressing type IV leaf glandular trichomes and secretion of acylsucroses from the wild tomato Solanum pimpinellifolium accession TO-937 into the genetic background of the whitefly- and virus-susceptible tomato cultivar Moneymaker. Results of preference bioassays with ABL 14-8 versus Moneymaker indicated that presence of type IV glandular trichomes and the production of acylsucrose deterred the landing and settling of B. tabaci on ABL 14-8. Moreover, electrical penetration graph studies indicated that B. tabaci adults spent more time in nonprobing activities and showed a reduced ability to start probing. Such behavior resulted in a reduced ability to reach the phloem. The superficial type of resistance observed in ABL 14-8 against B. tabaci probing significantly reduced primary and secondary spread of TYLCV.

  12. Novel subtypes (subgenotypes) of hepatitis B virus genotypes B and C among chronic liver disease patients in the Philippines.

    Science.gov (United States)

    Sakamoto, Tomoyuki; Tanaka, Yasuhito; Orito, Etsuro; Co, Jonard; Clavio, Joseph; Sugauchi, Fuminaka; Ito, Kiyoaki; Ozasa, Atsushi; Quino, Alvin; Ueda, Ryuzo; Sollano, Jose; Mizokami, Masashi

    2006-07-01

    Several hepatitis B virus (HBV) subtypes (subgenotypes), HBV/Aa (A1 : Asia/Africa), Ae (A2 : Europe), Bj (B1 : Japan) and Ba (B2 : Asia), have been reported with respect to clinical differences between patients infected with these subtypes (subgenotypes). HBV genotype distribution among patients with chronic liver diseases was investigated in the Philippines, where such studies have not been carried out previously. One hundred sera were obtained from such patients, consisting of 32 chronic hepatitis (CH), 37 cirrhosis and 31 hepatocellular carcinoma (HCC) patients. Nine complete genomes and 100 core promoter/precore genes of HBV were sequenced directly. Phylogenetic analyses revealed 51 HBV/A (Aa/A1), 22 HBV/B and 27 HBV/C strains. Interestingly, most HBV/C strains in the Philippines formed a specific cluster distinct from previous HBV/C strains (C1-4), indicating a novel subtype (subgenotype), HBV/C5. Moreover, most HBV/B strains fell within the specific cluster of the HBV/B subtype (subgenotype) B5, with viral characteristics of HBV/Ba (B2) carrying a recombination with HBV/C over the precore and core genes. Of the three genotypes, HBV/B and HBV/C were significantly more prevalent than HBV/A in cirrhosis and HCC patients (P<0.02). The prevalence of the core promoter mutations T1762/A1764 was higher in HCC patients with HBV/B and HBV/C. Multivariate analysis indicated that age [odds ratio (OR) 3.43; 95 % confidence interval (CI) 1.04-11.36; P=0.044] and the core promoter mutation (OR 14.08; 95% CI 3.62-4.74; P<0.001) were significant factors for HCC development. In conclusion, novel HBV subtypes (subgenotypes) C5 and B5 are prevalent in the Philippines, as well as HBV/Aa (A1).

  13. Comparative transcriptome analysis in Bemisia tabaci in response to tomato yellow leaf curl virus and development of ribonucleic acid interference to manage whitefly-transmitted viruses

    Science.gov (United States)

    The whitefly, Bemisia tabaci transmits over 300 plant viruses, with the majority of them belonging to the Begomovirus genus. Begomoviruses are obligately transmitted to a wide range of agriculture crops, resulting in the loss of billions of dollars annually, while jeopardizing food security worldwid...

  14. Transcriptome analysis of Bemisia tabaci during tomato yellow leaf curl virus acquisition and ribonucleic acid interference to manage whitefly-transmitted viruses

    Science.gov (United States)

    Over 300 viruses are transmitted by the whitefly, Bemisia tabaci, with 90% of them belonging to the genus, Begomovirus. Begomoviruses are obligately transmitted by whiteflies to a wide range of agriculture crops, resulting in billions of dollars lost annually, while jeopardizing food security worldw...

  15. Harm mechanism and method of prevention and cure of yellow leaf curl virus(TYLCV) in tomato%番茄黄化曲叶病毒的危害机理及防治方法

    Institute of Scientific and Technical Information of China (English)

    马妙芳

    2015-01-01

    通过对野生番茄的对比研究,发现并找到了番茄抗黄化曲叶病毒6个抗TYLCV的基因。分别是TY-1、TY-2、TY-3,TY-3a、TY-4、TY-5,这些抗TY病毒的基因都是从国外的材料中发现的。目前我国已经育成的抗TY病毒的番茄品种有1000多个。防治番茄黄化曲叶病毒主要措施;一是严把抗性鉴定关,主推抗性强的优良品种;二是加强田间管理,控制病毒传播;三是加强对中心病株的防治。%Through comparative study of wild tom ato, the six resistance gene of tomato yellow leaf curl virus (TYLCV)were found and they were TY-1、TY-2、TY-3,TY-3a、TY-4 and TY-5. These resistant genes were found in the material from abroad. At present our country had bred more than 1 000 tomato varieties with TY virus resistance.The main measures of prevention of tomato yellow leaf curl virus (TYLCV) were strengthening resistance identification, popularizing varieties of strong resistance, strengthening field management, controling the spread of the virus and strengthening the treatment of center infected plant.

  16. Molecular characterization of the spike and ORF3 genes of porcine epidemic diarrhea virus in the Philippines.

    Science.gov (United States)

    Kim, Yong Kwan; Cho, Yoon-Young; An, Byung-Hyun; Lim, Seong-In; Lim, Ji-Ae; Cho, In-Soo; Le, Van Phan; An, Dong-Jun

    2016-05-01

    Porcine epidemic diarrhea virus (PEDV) is a highly contagious enteric pathogen of swine. In the present study, we analyzed the spike genes and ORF3 genes of seven PEDV strains detected in Philippine pigs in June 2014. There are four major epitope regions in the spike glycoprotein: a CO-26K equivalent (COE) domain, SS2 and SS6 epitopes, and an epitope region recognized by the 2C10 monoclonal antibody. Analysis of Philippine strains revealed amino acid substitutions in the SS6 epitope region (LQDGQVKI to SQSGQVKI) of the S1 domain. Substitutions were also detected in the 2C10 epitope region (GPRLQPY to GPRFQPY) in the cytoplasmic domain. Phylogenetic analysis of the complete spike gene sequences from the seven strains revealed that they clustered within the G2 group but were distantly related to the North American and INDELs clusters. Interestingly, these strains were close to Vietnamese PEDVs on the ORF3 genetic tree and showed high (97.0-97.6 %) sequence identity to ORF3 genes at the nucleotide level.

  17. Resistance to tomato yellow leaf curl Thailand virus,TYLCTHV-[2] from Solanum habrochaites accession ‘L06112’ in F1 and BC1F1 generations

    Directory of Open Access Journals (Sweden)

    Julapark Chunwongse4

    2008-07-01

    Full Text Available Resistance to tomato yellow leaf curl disease caused by Tomato yellow leaf curl Thailand virus (TYLCTHV-[2] inwild tomato, Solanum habrochaites ‘L06112’ was investigated. The ‘L06112’ accession expressing the resistant phenotypewas crossed to the TYLCV-susceptible female parent, Seedathip3, to produce F1 hybrids. Parental polymorphism and hybrididentity were tested using 12 pairs of microsatellite markers for each chromosome. All markers were polymorphic betweenthe parents, but only markers SSR46, SSR115, SSR117 and SSR128 gave results suitable to assess hybrid relationships.Polymorphic bands were sharp, concise and distinguishable between hybrids and selfed plants. The stem cuttings of donorand recurrent parents, their F1 and BC1F1 were inoculated with TYLCTHV-[2] using viruliferous whiteflies. Diseaseresponse of the plants was evaluated by Enzyme-Linked Immunosorbent Assay (ELISA at 45 days post inoculation. Thedonor parental line showed complete resistance to TYLCTHV-[2] while the F1 and BC1F1 expressed various ELISA readingsfor TYLCTHV-[2] concentration. BC1F1; 04T105-7, 04T105-1, 04T105-10, 04T109-4 and 04T104-1 developed from thisstudy showed the high level of resistance to TYLCV, Thailand isolate.

  18. PCR detection of white spot syndrome virus (WSSV from farmed Pacific white shrimp (Litopenaeus vannamei in selected sites of the Philippines

    Directory of Open Access Journals (Sweden)

    Mary Beth B. Maningas

    2011-10-01

    Full Text Available Great losses caused by white spot syndrome virus (WSSV in shrimp culture have beenattributed to poor screening procedures in farms and the lack of sufficient access to specific pathogenfree brood stock. Thus, early detection of the virus is considered the best option for shrimp farmers. Thestudy, thus, assessed viral incidence in the Philippines and partially sequenced and characterized thePhilippine WSSV isolate with regards to other isolates in GenBank. Developed primers for PCR can detecttarget genes from 0.4 pg of DNA extract from shrimp samples. PCR detection revealed that 6.67 %(1/15 of market samples from Zambales are infected with WSSV. Shrimp samples from a local shop anda public market in General Santos City showed 46.67% (7/15 and 20% (3/15 WSSV-positive samplesrespectively. Shrimp sources from Capiz and Batangas, however, showed negative detection for WSV. Nosignificant difference in the number of infected samples from the sampling sites was found. Combineddetections reveal that the Philippines has a low infection rate of 14.67%. The study has partiallysequenced and characterized Philippine isolate. During the sampling period, most shrimps in GeneralSantos City were WSSV-positive by PCR detection.

  19. Making a friend from a foe: expressing a GroEL gene from the whitefly Bemisia tabaci in the phloem of tomato plants confers resistance to tomato yellow leaf curl virus.

    Science.gov (United States)

    Akad, F; Eybishtz, A; Edelbaum, D; Gorovits, R; Dar-Issa, O; Iraki, N; Czosnek, H

    2007-01-01

    Some (perhaps all) plant viruses transmitted in a circulative manner by their insect vectors avoid destruction in the haemolymph by interacting with GroEL homologues, ensuring transmission. We have previously shown that the phloem-limited begomovirus tomato yellow leaf curl virus (TYLCV) interacts in vivo and in vitro with GroEL produced by the whitefly vector Bemisia tabaci. In this study, we have exploited this phenomenon to generate transgenic tomato plants expressing the whitefly GroEL in their phloem. We postulated that following inoculation, TYLCV particles will be trapped by GroEL in the plant phloem, thereby inhibiting virus replication and movement, thereby rendering the plants resistant. A whitefly GroEL gene was cloned in an Agrobacterium vector under the control of an Arabidopsis phloem-specific promoter, which was used to transform two tomato genotypes. During three consecutive generations, plants expressing GroEL exhibited mild or no disease symptoms upon whitefly-mediated inoculation of TYLCV. In vitro assays indicated that the sap of resistant plants contained GroEL-TYLCV complexes. Infected resistant plants served as virus source for whitefly-mediated transmission as effectively as infected non-transgenic tomato. Non-transgenic susceptible tomato plants grafted on resistant GroEL-transgenic scions remained susceptible, although GroEL translocated into the grafted plant and GroEL-TYLCV complexes were detected in the grafted tissues.

  20. Viruses associated with epidemic hemorrhagic fevers of the Philippines and Thailand.

    Science.gov (United States)

    HAMMON, W M; RUDNICK, A; SATHER, G E

    1960-04-15

    Epidemiologic, clinical, and etiologic studies were carried out on a newly recognized, frequently fatal, pediatric disease syndrome which occurred in urban areas infested with Aedes aegypti mosquitoes. Four types of dengue virus (two of which are new), chikungunya virus, and another virus yet to be identified were isolated from the blood of patients. Dengue viruses, types 2 and 3, were isolated from the mosquitoes. Ample serologic confirmation was obtained of concurrent hemorrhagic fever and infection with one or more of these viruses. Thus, it was discovered that viruses of previously recognized types and of closely related new types apparently have etiologic roles in a new and highly dangerous epidemic disease syndrome.

  1. A new isolate of hepatitis B virus from the Philippines possibly representing a new subgenotype C6.

    Science.gov (United States)

    Cavinta, Lolita; Sun, Jianguang; May, Anja; Yin, Jianhua; von Meltzer, Markus; Radtke, Monika; Barzaga, Nina G; Cao, Guangwen; Schaefer, Stephan

    2009-06-01

    Hepatitis B virus (HBV) genotypes and subgenotypes show distinct geographical prevalence. A genotyping analysis of 28 samples from asymptomatic HBV carriers from the Philippines gave a distribution of HBV genotypes as expected from a previous study: 54% B (15/28), C5 18% (5/28), 14% D (4/28), 7% A1 (2/28). In addition, 7% (2/28) of the samples showed a double infection with genotypes B and D. One of the isolates sequenced completely, ph105, did not group into one of the known subgenotypes after phylogenetic analysis. Ph105 formed a separate clade in genotype C. With a genome length of 3,215 nt. and a serological subtype adr, ph105 exhibited the main features of most genotype C strains. However, ph105 differed by 4.1-7.2% from HBV subgenotypes C1 to C5 when comparing the nucleotide sequence of whole genomes. With only 4.1% difference ph105 was most closely related to subgenotype C2. SimPlot analysis gave no indication for recombination with known HBV genotypes. Ph105 fulfils all criteria for a new subgenotype C6.

  2. Diversity, Mutation and Recombination Analysis of Cotton Leaf Curl Geminiviruses.

    Directory of Open Access Journals (Sweden)

    Huma Saleem

    Full Text Available The spread of cotton leaf curl disease in China, India and Pakistan is a recent phenomenon. Analysis of available sequence data determined that there is a substantial diversity of cotton-infecting geminiviruses in Pakistan. Phylogenetic analyses indicated that recombination between two major groups of viruses, cotton leaf curl Multan virus (CLCuMuV and cotton leaf curl Kokhran virus (CLCuKoV, led to the emergence of several new viruses. Recombination detection programs and phylogenetic analyses showed that CLCuMuV and CLCuKoV are highly recombinant viruses. Indeed, CLCuKoV appeared to be a major donor virus for the coat protein (CP gene, while CLCuMuV donated the Rep gene in the majority of recombination events. Using recombination free nucleotide datasets the substitution rates for CP and Rep genes were determined. We inferred similar nucleotide substitution rates for the CLCuMuV-Rep gene (4.96X10-4 and CLCuKoV-CP gene (2.706X10-4, whereas relatively higher substitution rates were observed for CLCuMuV-CP and CLCuKoV-Rep genes. The combination of sequences with equal and relatively low substitution rates, seemed to result in the emergence of viral isolates that caused epidemics in Pakistan and India. Our findings also suggest that CLCuMuV is spreading at an alarming rate, which can potentially be a threat to cotton production in the Indian subcontinent.

  3. Study on the Color Analysis of Tomato Yellow Leaf Curl Virus Disease Based on Digital Images%基于数字图像的番茄黄化曲叶病毒病色彩分析研究

    Institute of Scientific and Technical Information of China (English)

    李俊; 陈振德

    2013-01-01

    为实现番茄黄化曲叶病毒病的快速无损监测,利用计算机图像处理技术对番茄叶片图像进行研究。在3种颜色系统中比较9种颜色参数,发现其中5种色彩参数存在显著差异,通过进一步的分布统计研究,发现了各参数的最优区分区间。其中G、Y、Cb3个值对感病叶片的区分率均达到70%以上,最优区分点分别在135、121和110,可以作为TYLCVD的特征参数应用于识别模型为后续研究识别模型提供重要的参数依据。试验结果表明,基于色彩分析法对番茄黄化曲叶病毒病进行识别是可行的。%In order to achieve rapid non-destructive monitoring of tomato yellow leaf curl virus disease, the author analyzed the images of tomato leaves using computer image processing technology. The author compared 9 color parameters in 3 color systems. The study showed that there were significant differences in 5 color parameters. In a further study on distribution statistics, it was found the optimal distinguish interval of each parameter. Each distinguish rate of G, Yand Cbwas more than 70%. The optimal distinguish interval of each 3 value was 135, 121 and 110. This provided important parameter basis for the follow-up study on identify model. The result showed that it’s feasible to identify tomato yellow leaf curl virus disease based on color analysis.

  4. Molecular Identification and Sequence Analysis of Tobacco Leaf Curl Begomovirus from Jember, East Java, Indonesia

    Directory of Open Access Journals (Sweden)

    SRI HENDRASTUTI HIDAYAT

    2008-03-01

    Full Text Available Begomovirus had been proved as the causal agent of leaf curl disease in tobacco in Indonesia, or commonly in Indonesia called as penyakit krupuk tembakau. Association of Begomovirus with the disease was further confirmed by sequence analysis. Amplification of the virus was conducted following whitefly (Bemisia tabaci Genn. transmission. Fragment of DNA 1.6 kb was amplified by polymerase chain reaction (PCR located within the replication initiator protein gene and coat protein gene (top region. Conserved sequence of stem loop region was found, included nonanucleotide sequence TAATATTAC present in all geminiviruses. Begomovirus associated with leaf curl disease in tobacco showed the closest relationship with Ageratum yellow vein virus - Zimbabwe, a strain of Tobacco leaf curl virus from Southern Africa. It was also known that Begomovirus associated with leaf curl disease in tobacco from Jember, East Java was different from other Indonesian Begomoviruses reported earlier.

  5. Gene expression profiling of the whitefly(Bemisia tabaci)Middle East-Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus-infected tobacco

    Institute of Scientific and Technical Information of China (English)

    Jun-Min Li; Yong-Ming Ruan; Fang-Fang Li; Shu-Sheng Liu; Xiao-Wei Wang

    2011-01-01

    Begomoviruses are exclusively transmitted by whitefly(Bemisia tabaci)in a circulative,non-propagative manner.The influences of begomoviruses on whitefly vector are complex with both direct and indirect effects.However,the molecular mechanisms underlying these effects are poorly understood and the transcriptional profiles of whitefly on healthy and virus-infected plants have not yet been studied.Using suppression subtractive hybridization,we investigated the differentially expressed genes in whitefly Middle East-Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus(TYLCCNV)infected tobacco.From the forward cDNA library,124 differentially expressed expression sequence tags(ESTs)were obtained which represent up-regulated genes in the whiteflies feeding on the infected tobacco.From the reverse library,112 ESTs were isolated which represent down-regulated genes.Among the up-regulated genes,we identified several genes that are probably involved in direct interaction between whitefly and TYLCCNV,including a 26/29-kDa proteinase that appears to participate in the elimination of foreign proteins,heparan sulfate proteoglycan which mediates the entry of several viruses into host cells and two genes of Rickettsia-a secondary endosymbiotic bacterium of whitefly.In addition,we identified a number of genes involved in metabolism,transcription and translation which might be the result of indirect effects of TYLCCNV on the whitefly via host plants.Collectively,our results suggest that TYLCCNV-infected tobacco changes the gene expression profiles of whitefly via both direct and indirect interactions.This study revealed a number of genes involved in whitefly-TYLCCNV-tobacco interactions and provided useful information for future study on this complex system.

  6. Tomato yellow leaf curl Sardinia virus-resistant tomato plants expressing the multifunctional N-terminal domain of the replication-associated protein show transcriptional changes resembling stress-related responses.

    Science.gov (United States)

    Lucioli, Alessandra; Berardi, Alessandra; Gatti, Francesca; Tavazza, Raffaela; Pizzichini, Daniele; Tavazza, Mario

    2014-01-01

    The N-terminal domain (amino acids 1-130) of the replication-associated protein (Rep130 ) of Tomato yellow leaf curl Sardinia virus (TYLCSV) retains the ability of full-length Rep to localize to the nucleus and to down-regulate C1 transcription when ectopically expressed in plants, both functions being required to inhibit homologous viral replication. In this study, we analysed the effect of Rep130 expression on virus resistance and the plant transcriptome in the natural and agronomically important host species of TYLCSV, Solanum lycopersicum. Tomato plants accumulating high levels of Rep130 were generated and proved to be resistant to TYLCSV. Using an in vitro assay, we showed that plant-expressed Rep130 also retains the catalytic activity of Rep, thus supporting the notion that this protein domain is fully functional. Interestingly, Rep130 -expressing tomatoes were characterized by an altered transcriptional profile resembling stress-related responses. Notably, the serine-type protease inhibitor (Ser-PI) category was over-represented among the 20 up-regulated genes. The involvement of Rep130 in the alteration of host mRNA steady-state levels was confirmed using a distinct set of virus-resistant transgenic tomato plants expressing the same TYLCSV Rep130 , but from a different, synthetic, gene. Eight genes were found to be up-regulated in both types of transgenic tomato and two encoded Ser-PIs. Four of these eight genes were also up-regulated in TYLCSV-infected wild-type tomato plants. Implications with regard to the ability of this Rep domain to interfere with viral infections and to alter the host transcriptome are discussed.

  7. Purification of tomato yellow leaf curl geminivirus.

    Science.gov (United States)

    Luisoni, E; Milne, R G; Vecchiati, M

    1995-07-01

    Attempts were made to find a good purification procedure for tomato yellow leaf curl virus (TYLCV), a dangerous and continuously spreading whitefly-transmitted germinivirus, up to now only partially purified. Electron microscopy, serology and spectrophotometry were used to evaluate different procedures. The scheme finally adopted was the following: collect leaves and stems from Nicotiana benthamiana graft-infected 45-60 days previously (5-10 g/plant); homogenize with 0.5 M phosphate buffer pH 6 containing 2.5 mM NaEDTA, 10 mM Na2SO3, 0.1% 2-mercaptoethanol, 1% Triton X-100 and 0.1% Driselase (3-4 ml of buffer for each g of material); incubate overnight on ice with gentle agitation; filter; emulsify with 15% cold chloroform; centrifuge at low speed; ultracentrifuge supernatant; resuspend pellets in 0.5 M phosphate buffer pH 7 containing 2.5 mM NaEDTA; centrifuge at low speed; repeat resuspension of the pellets and low-speed centrifugation; ultracentrifuge the pooled supernatant on a Cs2SO4 gradient (e.g. for 5 h at 41,000 rpm); collect the virus band and dialyse or ultracentrifuge the virus. The virus yield was 5-10 mg per kg of tissue.

  8. Manila, Philippines

    National Research Council Canada - National Science Library

    Fatima Arkin

    2015-01-01

    .... Not odd assignments, considering that in the Philippine capital and its suburbs, roughly 10 percent of the workforce is unemployed and, in Manila alone, about one-third of residents live in slums...

  9. The C2 Protein from the Geminivirus Tomato Yellow Leaf Curl Sardinia Virus Decreases Sensitivity to Jasmonates and Suppresses Jasmonate-Mediated Defences

    Directory of Open Access Journals (Sweden)

    Tábata Rosas-Díaz

    2016-01-01

    Full Text Available An increasing body of evidence points at a role of the plant hormones jasmonates (JAs in determining the outcome of plant-virus interactions. Geminiviruses, small DNA viruses infecting a wide range of plant species worldwide, encode a multifunctional protein, C2, which is essential for full pathogenicity. The C2 protein has been shown to suppress the JA response, although the current view on the extent of this effect and the underlying molecular mechanisms is incomplete. In this work, we use a combination of exogenous hormone treatments, microarray analysis, and pathogen infections to analyze, in detail, the suppression of the JA response exerted by C2. Our results indicate that C2 specifically affects certain JA-induced responses, namely defence and secondary metabolism, and show that plants expressing C2 are more susceptible to pathogen attack. We propose a model in which C2 might interfere with the JA response at several levels.

  10. Biology and interactions of two distinct monopartite begomoviruses and betasatellites associated with radish leaf curl disease in India

    Directory of Open Access Journals (Sweden)

    Singh AK

    2012-02-01

    Full Text Available Abstract Background Emerging whitefly transmitted begomoviruses are major pathogens of vegetable and fibre crops throughout the world, particularly in tropical and sub-tropical regions. Mutation, pseudorecombination and recombination are driving forces for the emergence and evolution of new crop-infecting begomoviruses. Leaf curl disease of field grown radish plants was noticed in Varanasi and Pataudi region of northern India. We have identified and characterized two distinct monopartite begomoviruses and associated beta satellite DNA causing leaf curl disease of radish (Raphanus sativus in India. Results We demonstrate that RaLCD is caused by a complex of two Old World begomoviruses and their associated betasatellites. Radish leaf curl virus-Varanasi is identified as a new recombinant species, Radish leaf curl virus (RaLCV sharing maximum nucleotide identity of 87.7% with Tomato leaf curl Bangladesh virus-[Bangladesh:2] (Accession number AF188481 while the virus causing radish leaf curl disease-Pataudi is an isolate of Croton yellow vein mosaic virus-[India] (CYVMV-IN (Accession number AJ507777 sharing 95.8% nucleotide identity. Further, RDP analysis revealed that the RaLCV has a hybrid genome, a putative recombinant between Euphorbia leaf curl virus and Papaya leaf curl virus. Cloned DNA of either RaLCV or CYVMV induced mild leaf curl symptoms in radish plants. However, when these clones (RaLCV or CYVMV were individually co-inoculated with their associated cloned DNA betasatellite, symptom severity and viral DNA levels were increased in radish plants and induced typical RaLCD symptoms. To further extend these studies, we carried out an investigation of the interaction of these radish-infecting begomoviruses and their associated satellite, with two tomato infecting begomoviruses (Tomato leaf curl Gujarat virus and Tomato leaf curl New Delhi virus. Both of the tomato-infecting begomoviruses showed a contrasting and differential interaction with

  11. 抗番茄黄化卷叶病毒番茄新品种'科大204'%A New Tomato Hybrid'Keda 204'with Resistance to Tomato yellow leaf curl virus

    Institute of Scientific and Technical Information of China (English)

    宋建军; 艾鹏飞; 李振侠; 仇燕; 李敏

    2011-01-01

    '科大204' 番茄是以优良自交系'TP025-2'为母本,'869-1'为父本杂交育成的抗番茄黄化卷叶病毒的新品种.生长势强,无限生长类型,7~8片真叶着生第一花序.果实扁圆形,单果质量200~250g,成熟果粉红色,中等硬度,口感好,品质优良.抗番茄黄化卷叶病毒,兼抗枯萎病、叶霉病和TMV.中早熟,平均产量121.8 t·hm-2,适宜北方保护地早春和秋延后栽培.%‘Keds 204’ is a new pink tomato hybrid resistant to Tomato yellow leaf curl virus (TYLCV), which is developed by crossing ‘TP025-2’ × ‘869-1’ . The hybrid grows strongly with indeterminate plant type and mid-early maturity. The matured fruits are oblate in shape, pink in color with moderate firmness and good quality. The single fruit weight ranges from 200 g to 250 g. Especially, it is resistance to TYLCV, as well as fusarium wilt, leaf mould and TMV. The average yield of ‘Keda 204’was 121.8 t. hm-2 as a result of multiple experiments. The hybrid is suitable for cultivation in early spring and late autumn under greenhouse conditions in northern China.

  12. A review of the mechanisms and components that determine the transmission efficiency of Tomato yellow leaf curl virus (Geminiviridae; Begomovirus) by its whitefly vector.

    Science.gov (United States)

    Ghanim, Murad

    2014-06-24

    Begomoviruses are a group of icosahedral single stranded DNA viruses exclusively transmitted by the sweet potato whitefly Bemisia tabaci in a persistent, circulative manner. In this mode of transmission, begomoviruses are acquired by their insect vector as intact virions from the plant phloem, move along the food canal, foregut and esophagus and reach the midgut where they are absorbed into the hemolymph via the filter chamber. The filter chamber is the site where most of the ingested food is filtered, and the first site where the majority of begomoviruses appear to be translocated into the hemolymph via unknown proteins or receptors. Transport from the filter chamber to the hemolymph is aided by a Heat Shock Protein 70. Virus particles not translocated across the filter chamber circulate in the midgut loop but it is not known whether absorption into the hemolymph occurs along this loop. Localization studies have confirmed that begomoviruses are not associated with the hindgut and absorption of virions in this organ is unlikely. In the hemolymph, virions have been shown to interact with a GroEL chaperone produced by the whitefly's endosymbiontic bacteria for ensuring their safe journey to the salivary glands. Virions penetrate the primary salivary glands via unknown proteins or receptors and are transported and secreted outside the whitefly to the plant with salivary secretions. Several recent studies have demonstrated the implications of insect and endosymbiont proteins such as the heat shock protein 70 and the bacterial GroEL protein, in the transmission of begomoviruses by B. tabaci. Additional studies attempting to identify other proteins that aid or interact with begomoviruses along their circulation pathway in the whitefly are reviewed in this paper.

  13. Analysis of the entire nucleotide sequence of hepatitis B virus genotype B in the Philippines reveals a new subgenotype of genotype B.

    Science.gov (United States)

    Nagasaki, Futoshi; Niitsuma, Hirofumi; Cervantes, Julieta G; Chiba, Masanori; Hong, Shan; Ojima, Toshiaki; Ueno, Yoshiyuki; Bondoc, Edgardo; Kobayashi, Koju; Ishii, Motoyasu; Shimosegawa, Tooru

    2006-05-01

    The entire nucleotide sequences were determined for hepatitis B virus (HBV) genotype B (HBV/B) genomes extracted from five patients in the Philippines and designated GenBank AB219426, AB219427, AB219428, AB219429 and AB219430. The serotype of the first four isolates was ayw and that of GenBank AB219430 was adw. Divergences of entire sequences were 1.0-2.0 % between the first four isolates and 3.8-4.2 % between these four and GenBank AB219430. Phylogenetic-tree analysis revealed that, worldwide, HBV/B comprises five subgenotypes: B1, B2, B3, B4 and the new Philippines group, designated B5. Divergences of the entire genome sequences between four isolates in subgenotype B5 and isolates from other countries (subgenotypes) were 4.4-4.8 % with Vietnam (B4), 2.9-3.5 % with Indonesia (B3), 4.7-5.1 % with China (B2) and 5.4-6.0 % with Japan (B1). Similarly, GenBank AB219430 showed the lowest divergences: 3.4 % with the isolate from Indonesia (B3), 5.0 % with Vietnam (B4), 5.4 % with China (B2) and 6.1 % with Japan (B1). This is the first report of entire nucleotide sequences of HBV/B from the Philippines and the results show that these sequences belong to a new subgenotype, B5. The present study identified that HBV/B isolates throughout the world are divided genetically into five subgenotypes, the relationships between geographical distances and the genetic distances of HBV/B being well-correlated.

  14. Exploitation of Solanum chilense and Solanum peruvianum in tomato breeding for resistance to Tomato yellow leaf curl disease.

    OpenAIRE

    Julián Rodríguez, Olga

    2014-01-01

    Among viral diseases affecting cultivated tomato, Tomato yellow leaf curl disease (TYLCD) is one of the most devastating. This disease is caused by a complex of viruses of which Tomato yellow leaf curl virus (TYLCV) is regarded as the most important species. Current control strategies to fight viral diseases in tomato are mainly based on genetic resistance derived from wild relatives. In the present thesis, resistance derived from S. chilense and S. peruvianum has been exploited in breeding f...

  15. 番茄黄化曲叶病毒对温室白粉虱适合度的影响%Effects of tomato yellow leaf curl virus on the fitness of greenhouse whitefly

    Institute of Scientific and Technical Information of China (English)

    付雪; 赵奎军; 赵娜; 谭巍; 裴海英; 闫春秀; 叶乐夫

    2015-01-01

    Objectives] The greenhouse whitefly is a major insect pest of multiple horticultural and vegetable crops in Northern China. The sweet potato whitefly species complex is another pest species that has recently spread into parts of Heilongjiang Province as a result of the flower trade and is rapidly replacing native species to become the new dominant species. The tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted by the sweet potato whitefly that can cause serious damage to plant crops. There has been little recent research on the effect of this virus on the greenhouse whitefly, or on competition between these two whitefly species. [Methods] We observed the population dynamics of both whitefly species on infected and un-infected tomato plants and related these to host plant’s morphological and physical indices. [Results] 1) Tomato plants infested by the sweet potato whitefly became short and thin with more root biomass; 2) Plants infested with the greenhouse whitefly became a little shorter but thicker; 3) Infected plants became shorter and thicker (or thinner), without significant root variance; 4) Compared with infested plants, plants that were both infected with the virus and infested with the greenhouse whitefly became shorter and thinner with a sharp decrease in root biomass, whereas those infested with the sweet potato whitefly showed much reduced symptoms; 5) Different enzymes responded differently to plant infection; higher AchE activity injured plants but higher GST was beneficial. [Conclusion] Infestation by the sweet potato whitefly injured tomato seedlings, but the greenhouse whitefly didn’t directly affect tomato plants; virus infection reduces the plant response to sweet potato whitefly infestation, but enhances the response to infestation by the greenhouse whitefly, that is to say, the growth and development of infected plants infested by the greenhouse whitefly was significantly suppressed. The sweet potato whitefly laid less eggs

  16. 植物源抗病毒剂丁香酚防治番茄黄化曲叶病毒病的效果分析%Control Effect of Botanical Virucide Eugenol against Tomato Yellow Leaf Curl Virus Disease

    Institute of Scientific and Technical Information of China (English)

    张晓艳; 陈浩; 张晓阳; 石志琦

    2013-01-01

    In this study, the field plot experiment was carried out to investigate the control effect of eugenol against tomato yellow leaf curl virus disease.The results showed that the control effect of using 20%eugenol EW for 4 times from tomato seedling stage was the highest (70.96%); the control effect of applying 20% eugenol EW for 3 times from tomato flowering stage was 65.07%;the control effect of first applying 20%guanidine・ copper wettable powder (WP) for 2 times and then using 20%euge-nol EW for 2 times was 60.31%, which was not significantly different from that of applying 20%eugenol EW;the continuous appli-cation of 20%guanidine・ copper WP for 4 times showed the lowest control effect (53.57%), which was significantly lower than that of all the above treatments.%采用田间小区试验的方法,研究丁香酚防治番茄黄化曲叶病毒病的效果,结果表明:从番茄苗期开始,施用4次20%丁香酚水乳剂的防效最高,为70.96%;从花期开始施用3次20%丁香酚水乳剂的防效为65.07%;先施用2次20%吗胍・铜可湿性粉剂,再施用2次20%丁香酚水乳剂的防效为60.31%,与20%丁香酚水乳剂处理没有显著差异;连续施用4次20%吗胍・铜可湿性粉剂的防效最低,为53.57%,显著低于其他处理。

  17. Spatial and temporal diversity of begomoviral complexes in papayas with leaf curl disease.

    Science.gov (United States)

    Singh-Pant, P; Pant, P; Mukherjee, S K; Mazumdar-Leighton, S

    2012-07-01

    Old World, monopartite begomoviruses associated with satellite DNA β were observed in papaya showing symptoms of leaf curl disease sampled randomly over five years from within a radius of 250 km in north-central India. Three groups of DNA A sequences were evident. One group resembled chili leaf curl virus infecting tomatoes (ChiLCuV). Another group resembled tomato leaf curl New Delhi virus (ToLCuNDV). The third group was novel (tentatively named papaya leaf crumple virus, PaLCrV), with less than 89% identity to known begomovirus sequences in the GenBank database. At least seven DNA A sequences were putative recombinants. The AC4-encoding regions exhibited highest numbers of non-synonymous substitutions. Most DNA β sequences resembled tomato leaf curl virus-associated DNA βs. A few DNA β sequences were similar to that of croton yellow vein mosaic virus-associated DNA β (CroYVMVβ). One DNA β sequence was novel and showed papayas grown in plantations, kitchen gardens and feral patches in the region are vulnerable to disease outbreak. No geographic or temporal patterns were discernable in the distribution of these viruses.

  18. Preliminary Functional Studies on AC2, a Novel Trans-acting Factor from Cotton Leaf Curl Virus%棉花曲叶病毒反式作用因子AC2的功能初探

    Institute of Scientific and Technical Information of China (English)

    谢迎秋; 孟蒙; 朱祯; 吴茜; 徐鸿林; 刘玉乐

    2001-01-01

    Studies on tomato golden mosaic virus and African cassava mosaicvirus suggested that virion sense promoter was trans-activated in transient expression by AC2 encoded by geminivirus. The AC2 gene fragment of cotton leaf curl virus (CLCuV) was obtained from total DNA of CLCuV infected tobacco leaves by polymerase chain reaction, and the amplified DNA fragment was cloned into vector. Transient expression vectors were constructed by fusing the AC2 gene fragment with CaMV 35S promoter and nopaline terminator. These constructs were delivered into tobacco (Nicotiana tabacum L.) and cotton (Gossypium hirsutum L.) leaf cells for transient expression by particle bombardment. Results indicated that activity of virion sense promoter was activated by AC2 and increased remarkably. However, the activity of trans-activated virion sense promoter was still lower than that of complementary sense promoter. Expression pattern of trans-activated virion sense promoter was similar to that of complementary sense promoter with the high activity in both mesophyll and vascular of leaf vein. In this paper, the expression behavior of AC2 in Agrobacterium-mediated transgenic plants was also discussed.%有关非洲木薯花叶病毒(ACMV)、番茄金色花叶病毒(TGMV)的研究表明,双生病毒编码的反式作用因子AC2反式激活病毒链基因启动子的瞬时表达。以棉花曲叶病毒(CLCuV)侵染的烟草叶片组织总DNA为模板,通过聚合酶链反应扩增CLCuV的AC2基因片段并插入克隆载体。将AC2置于CaMV35S启动子下构建了瞬时表达载体。通过基因枪法将质粒载体导入烟草(Nicotianatabacum L.)和棉花(Gossypium hirsutum L.)叶片细胞中进行瞬时表达,结果表明,在反式作用因子AC2的激活下,病毒链基因启动子驱动的GUS活性明显增强,然而激活后的病毒链基因启动子的活性仍低于互补链基因方向启动子;其表达方式与互补链基因启动子相似,即在叶肉及叶脉

  19. 基于微流控芯片电泳的番茄黄化曲叶病毒快速检测%Microfluidic Electrophoresis Detection of Tomato yellow leaf curl virus(TYLCV)

    Institute of Scientific and Technical Information of China (English)

    余明芬; 曾洪梅; 钟润涛; 赵小明; 邱德文

    2014-01-01

    控芯片电泳进行比较,确立了后者在核酸检测方面的应用价值;通过微流控芯片电泳对TYLCV PCR产物的检测,建立了基于微流控芯片电泳的TYLCV快速检测方法,为TYLCV的快速检测提供新的技术支持。%Objective]The objectives of this study are to explore the effect of microfluidic electrophoresis on detection of PCR products, build a detection method for Tomato yellow leaf curl virus (TYLCV) by electrophoresis in microfluidic chip, and to remedy the defect in reagent consumption, long time, lack of safety of agarose gel electrophoresis.[Method]Primers in relatively stable positions of TYLCV genome were designed, some primers in references were taken into account, and these selected primers were verified. TYLCV primers were screened out based on the criterion of specificity, stability, and sensitivity. DNA standardsφX174/BsuR I (HaeⅢ) marker was subjected to agarose gel electrophoresis and microfluidic electrophoresis, and the two methods were compared in supplies, time-consumption and sensitivity to confirm the value of microfluidic electrophoresis in nucleic acid detection. In order to evaluate the value of microfluidic electrophoresis in virus detection, the PCR amplification products of one pair of selected primers on the actual samples were processed by microfluidic electrophoresis.[Result]Fourteen pairs of TYLCV primers were screened out, 2 pairs came from the literatures and the other 12 pairs were designed in this study. Each pair of primers could meet the requirement for microfluidic detection. TYLCV-T was chosen from these primers for the subsequent study. By comparison of agarose gel electrophoresis and microfluidic electrophoresis, the time consumption and reagent consumption of microfluidic electrophoresis were 1/10 and 1/8 of those of agarose gel electrophoresis, respectively. The detection sensitivity of microfluidic electrophoresis was at least 103 times higher than that of agarose gel

  20. Genetic control of leaf curl in maize.

    Science.gov (United States)

    Entringer, G C; Guedes, F L; Oliveira, A A; Nascimento, J P; Souza, J C

    2014-03-17

    Among the many implications of climatic change on agriculture, drought is expected to continue to have a major impact on agribusinesses. Leaf curling is an anatomical characteristic that might be potentially used to enhance plant tolerance to water deficit. Hence, we aimed to study the genetic control of leaf curl in maize. From 2 contrasting inbred lines for the trait, generations F1, F2, and the backcrosses were obtained. All of these generations were evaluated in a randomized block design with 2 replicates. Leaf curl samples were collected from 3 leaves above the first ear at the tasseling stage, and quantified by dividing the width of the leaf blade with natural curling against its extended width. The mean and variance components were estimated by the weighted least square method. It was found that the trait studied has predominance of the additive effects, with genetic control being attributed to few genes that favor selection and exhibit minimal influence from the environment.

  1. Suppressors of RNA silencing encoded by tomato leaf curl betasatellites

    Indian Academy of Sciences (India)

    Richa Shukla; Sunita Dalal; V G Malathi

    2013-03-01

    Virus encoded RNA-silencing suppressors (RSSs) are the key components evolved by the viruses to counter RNA-silencing defense of plants. Whitefly-transmitted begomoviruses infecting tomato crop code for five different proteins, ORF AC4, ORF AC2 and ORF AV2 in DNA-A component, ORF BV1 in DNA-B and ORF C1 in satellite DNA which are predicted to function as silencing suppressors. In the present study suppressor function of ORF C1 of three betasatellites Tomato leaf curl Bangalore betasatellite ToLCBB-[IN:Hess:08], Cotton leaf curl Multan betasatellite CLCuMB–[IN:Sri:02] and Luffa leaf distortion betasatellite LuLDB-[IN:Lu:04] were examined. Agroinfiltration of GFP-silenced Nicotiana tabaccum cv. Xanthi with the cells expressing C1 protein resulted in reversal of silenced GFP expression. GFP-siRNA level was more than 50-fold lower compared to silenced plants in plants infiltrated with C1 gene from ToLCBB. However, in the case of 35S-C1 CLCuMB and 35S-C1 LuLDB construct, although GFP was expressed, siRNA level was not reduced, indicating that the step at which C1 interfere in RNA-silencing pathway is different.

  2. Suppressors of RNA silencing encoded by tomato leaf curl betasatellites.

    Science.gov (United States)

    Shukla, Richa; Dalal, Sunita; Malathi, V G

    2013-03-01

    Virus encoded RNA-silencing suppressors (RSSs) are the key components evolved by the viruses to counter RNA-silencing defense of plants. Whitefly-transmitted begomoviruses infecting tomato crop code for five different proteins, ORF AC4, ORF AC2 and ORF AV2 in DNA-A component, ORF BV1 in DNA-B and ORF beta C1 in satellite DNA beta which are predicted to function as silencing suppressors. In the present study suppressor function of ORF beta C1 of three betasatellites Tomato leaf curl Bangalore betasatellite ToLCBB-[IN:Hess:08], Cotton leaf curl Multan betasatellite CLCuMB-[IN:Sri:02] and Luffa leaf distortion betasatellite LuLDB-[IN:Lu:04] were examined. Agroinfiltration of GFP-silenced Nicotiana tabaccum cv. Xanthi with the cells expressing betaC1 protein resulted in reversal of silenced GFP expression. GFP-siRNA level was more than 50-fold lower compared to silenced plants in plants infiltrated with betaC1 gene from ToLCBB. However, in the case of 35S-beta C1 CLCuMB and 35S- beta C1 LuLDB construct, although GFP was expressed, siRNA level was not reduced, indicating that the step at which beta C1 interfere in RNA-silencing pathway is different.

  3. Chikungunya Virus Infections Among Patients with Dengue-Like Illness at a Tertiary Care Hospital in the Philippines, 2012-2013.

    Science.gov (United States)

    Velasco, John Mark; Valderama, Maria Theresa; Lopez, Maria Nila; Chua, Domingo; Latog, Rene; Roque, Vito; Corpuz, June; Klungthong, Chonticha; Rodpradit, Prinyada; Hussem, Kittinun; Poolpanichupatam, Yongyuth; Macareo, Louis; Fernandez, Stefan; Yoon, In-Kyu

    2015-12-01

    Chikungunya virus (CHIKV) often co-circulates with dengue virus (DENV). A cross-sectional surveillance study was conducted at a tertiary hospital in Manila, Philippines, to describe the prevalence and characteristics of DENV and CHIKV infections among patients seeking care for dengue-like illness. Acute blood samples from patients ≥ 6 months of age clinically diagnosed with dengue from November 2012 to December 2013 underwent reverse transcription polymerase chain reaction (RT-PCR) to detect DENV and CHIKV RNA. A total of 118 patients with clinically diagnosed dengue (age range = 1-89 years, mean = 22 years; male-to-female ratio = 1.51) were tested by DENV RT-PCR; 40 (34%) were DENV PCR-positive (age range = 1-45 years, mean = 17 years). All DENV serotypes were detected: 11 (28%) DENV-1, 6 (15%) DENV-2, 6 (15%) DENV-3, and 17 (42%) DENV-4. Of 112 patients clinically diagnosed with dengue and tested by CHIKV RT-PCR, 11 (10%) were CHIKV PCR-positive (age range = 2-47 years, mean = 20.3 years). No coinfections were detected. Presenting signs/symptoms did not differ between DENV- and CHIKV-positive cases. Sequencing of envelope 1 gene from two CHIKV PCR-positive samples showed Asian genotype. This study highlights the potential for misdiagnosis of medically attended CHIKV infections as DENV infection and the difficulty in clinically differentiating dengue and chikungunya based on presenting signs/symptoms alone. This underscores the necessity for diagnostic laboratory tests to distinguish CHIKV infections in the background of actively co-circulating DENV.

  4. High rate of subclinical chikungunya virus infection and association of neutralizing antibody with protection in a prospective cohort in the Philippines.

    Directory of Open Access Journals (Sweden)

    In-Kyu Yoon

    2015-05-01

    Full Text Available Chikungunya virus (CHIKV is a globally re-emerging arbovirus for which previous studies have indicated the majority of infections result in symptomatic febrile illness. We sought to characterize the proportion of subclinical and symptomatic CHIKV infections in a prospective cohort study in a country with known CHIKV circulation.A prospective longitudinal cohort of subjects ≥6 months old underwent community-based active surveillance for acute febrile illness in Cebu City, Philippines from 2012-13. Subjects with fever history were clinically evaluated at acute, 2, 5, and 8 day visits, and at a 3-week convalescent visit. Blood was collected at the acute and 3-week convalescent visits. Symptomatic CHIKV infections were identified by positive CHIKV PCR in acute blood samples and/or CHIKV IgM/IgG ELISA seroconversion in paired acute/convalescent samples. Enrollment and 12-month blood samples underwent plaque reduction neutralization test (PRNT using CHIKV attenuated strain 181/clone25. Subclinical CHIKV infections were identified by ≥8-fold rise from a baseline enrollment PRNT titer 50 years old. Baseline CHIKV PRNT titer ≥10 was associated with 100% (95%CI: 46.1, 100.0 protection from symptomatic CHIKV infection. Phylogenetic analysis demonstrated Asian genotype closely related to strains from Asia and the Caribbean.Subclinical infections accounted for a majority of total CHIKV infections. A positive baseline CHIKV PRNT titer was associated with protection from symptomatic CHIKV infection. These findings have implications for assessing disease burden, understanding virus transmission, and supporting vaccine development.

  5. Chikungunya Virus Infections among Patients with Dengue-Like Illness at a Tertiary Care Hospital in the Philippines, 2012–2013

    Science.gov (United States)

    Velasco, John Mark; Valderama, Maria Theresa; Lopez, Maria Nila; Chua, Domingo; Latog, Rene; Roque, Vito; Corpuz, June; Klungthong, Chonticha; Rodpradit, Prinyada; Hussem, Kittinun; Poolpanichupatam, Yongyuth; Macareo, Louis; Fernandez, Stefan; Yoon, In-Kyu

    2015-01-01

    Chikungunya virus (CHIKV) often co-circulates with dengue virus (DENV). A cross-sectional surveillance study was conducted at a tertiary hospital in Manila, Philippines, to describe the prevalence and characteristics of DENV and CHIKV infections among patients seeking care for dengue-like illness. Acute blood samples from patients ≥ 6 months of age clinically diagnosed with dengue from November 2012 to December 2013 underwent reverse transcription polymerase chain reaction (RT-PCR) to detect DENV and CHIKV RNA. A total of 118 patients with clinically diagnosed dengue (age range = 1–89 years, mean = 22 years; male-to-female ratio = 1.51) were tested by DENV RT-PCR; 40 (34%) were DENV PCR-positive (age range = 1–45 years, mean = 17 years). All DENV serotypes were detected: 11 (28%) DENV-1, 6 (15%) DENV-2, 6 (15%) DENV-3, and 17 (42%) DENV-4. Of 112 patients clinically diagnosed with dengue and tested by CHIKV RT-PCR, 11 (10%) were CHIKV PCR-positive (age range = 2–47 years, mean = 20.3 years). No coinfections were detected. Presenting signs/symptoms did not differ between DENV- and CHIKV-positive cases. Sequencing of envelope 1 gene from two CHIKV PCR-positive samples showed Asian genotype. This study highlights the potential for misdiagnosis of medically attended CHIKV infections as DENV infection and the difficulty in clinically differentiating dengue and chikungunya based on presenting signs/symptoms alone. This underscores the necessity for diagnostic laboratory tests to distinguish CHIKV infections in the background of actively co-circulating DENV. PMID:26416109

  6. Flange Curling in Cold Formed Profiles

    DEFF Research Database (Denmark)

    Jönsson, Jeppe; Ramonas, Gediminas

    2012-01-01

    The non-linear flange curling phenomenon in cold formed profiles is the tendency of slender flanges to deform towards the neutral axis for increasing flexural curvature. Based on Braziers work, Winter proposed a simple engineering formula for determination of the local flange deformation towards...

  7. Resistance to tomato yellow leaf curl virus-Thailand isolate (TYLCTHV-[2] and markers loci association in BC2F1 population from a cross between Seedathip 3 and a wild tomato, Solanum habrochaites ‘L06112’ clone no.1

    Directory of Open Access Journals (Sweden)

    Uraiwan Pongpayaklers

    2012-02-01

    Full Text Available The BC2F1 population from a cross between wild tomato, Solanum habrochaites ‘L06112’ and recurrent susceptiblevariety, Seedathip 3 was investigated for a resistance to Tomato yellow leaf curl Thailand virus (TYLCTHV-[2]. Five stemcuttings from each of the 196 lines were inoculated with TYLCTHV-[2] using viruliferous whiteflies as the inoculation vector.Disease response was recorded weekly intervals and scored for three weeks according to the severity of the symptoms. Thepresence of TYLCTHV-[2] was confirmed by enzyme-linked immunosorbent assay (ELISA at three weeks after inoculation.ELISA readings showed a normal distribution for the BC2F1 population ranging from 0.055 (resistant to 0.930 (susceptible.DNA samples from BC2F1 population were analyzed for genes and markers association; Ty-2 on chromosome 11 using AVRDCprimer number 11-090.0 (TG105A, and Ty-3 on chromosome 6 using marker C2_At3g11210. Results showed that Ty-2 waslost during stepwise selection at BC1F1 generation, while Ty-3 showed no relationship to marker C2_At3g11210 and theamount of virus detected from ELISA reading. This indicated that the TYLCTHV-[2] resistant phenotype response in theBC2F1 population neither came from Ty-2 nor linked to the Ty-3 gene.

  8. Deformation behavior of curling strips on tearing tubes

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Ji Won; Kwon, Tae Soo; Jung, Hyun Seung; Kim, Jin Sung [Dept. of Robotics and Virtual Engineering, Korea University of Science and Technology, Seoul (Korea, Republic of)

    2015-10-15

    This paper discusses the analysis of the curl deformation behavior when a dynamic force is applied to a tearing tube installed on a flat die to predict the energy absorption capacity and deformation behavior. The deformation of the tips of the curling strips was obtained when the curl tips and tube body are in contact with each other, and a formula describing the energy dissipation rate caused by the deformation of the curl tips is proposed. To improve this formula, we focused on the variation of the curl radius and the reduced thickness of the tube. A formula describing the mean curl radius is proposed and verified using the curl radius measurement data of collision test specimens. These improved formulas are added to the theoretical model previously proposed by Huang et al. and verified from the collision test results of a tearing tube.

  9. Preparation of Curled Micro bers by Electrospinning with Tip Collector

    Institute of Scientific and Technical Information of China (English)

    TANG Cheng-Chun; CHEN Jun-Chi; LONG Yun-Ze; YIN Hong-Xing; SUN Bin; ZHANG Hong-Di

    2011-01-01

    We report on curled polyvinylpyrrolidone (PVP) microfibers fabricated by a modified electrospinning with a small nail as the tip collector. PVP (45 wt%) ethanol solution is electrospun under different working voltages ranging from 10 to 15, 20, 30 and 40kV. It is found that with the increase of working voltage, the proportion of the curled fibers increases and the uniformity of the curled fibers improves, as well as the repeat distance of the curled structures reducing. Particularly, some curled fibers develop into helical structures under relatively high voltages. Further analyses indicate that the formation mechanism for the curled polymer fibers can be ascribed to electrical driven bending instability and/or mechanical jet buckling when hitting the collector surface. This modified electrospinning technique may be a cost-effective approach for the mass production of curled microfibers.%@@ We report on curled polyvinylpyrrolidone(PVP) microfibers fabricated by a modified electrospinning with a small nail as the tip collector.PVP(45 wt%) ethanol solution is electrospun under different working voltages ranging from 10 to 15,20,30 and 40kV.It is found that with the increase of working voltage,the proportion of the curled fibers increases and the uniformity of the curled fibers improves,as well as the repeat distance of the curled structures reducing.Particularly,some curled fibers develop into helical structures under relatively high voltages.Further analyses indicate that the formation mechanism for the curled polymer fibers can be ascribed to electrical driven bending instability and/or mechanical jet buckling when hitting the collector surface.This modified electrospinning technique may be a cost-effective approach for the mass production of curled microfibers.

  10. Prevention and Control of Field Planting with Ditch,Cooling and Moisture Retention on Tomato Yellow Leaf Curl Virus Disease%“沟栽降温保湿法”定植防控番茄黄化曲叶病毒病的研究初报

    Institute of Scientific and Technical Information of China (English)

    郭建武; 姜冬仓; 薛爱利

    2011-01-01

    近几年,我国夏季番茄生产中黄化曲叶病毒病为害日趋严重,北方地区由于夏季极端高温天数不断增多,导致夏秋番茄黄化曲叶病毒病的发病规模迅速扩大,严重为害夏秋淡季番茄的生产。目前该病害尚无有效的根治措施,只能是预防为主。以夏秋茬大棚番茄为试材,采用对比设计,设沟栽降温保湿法(简称为"沟栽法")定植和平畦定植(CK)2种处理方式,研究了沟栽法定植对番茄黄化曲叶病毒病的防控效果。结果表明:当温室大棚内平均最高温度为33~36℃时,利用"沟栽法"定植可使沟内番茄根际5~25 cm地温稳定在28℃以下,较平畦栽植平均降低3.1℃;沟内土壤相对湿度60%~80%时,5 cm地温均可降至28℃以下,降温效果明显。同时,随着根际土壤温度的降低,番茄黄化曲叶病毒病的发生率极显著降低(仅为8.2%),较平畦栽植降幅达89.62%,差异达极显著水平;病情指数降低了27.36%,差异达显著水平。"沟栽降温保湿法"定植能够有效防控番茄黄化曲叶病毒病的发生。在番茄生产上,采取"沟栽降温保湿法"定植可以作为防控番茄黄化曲叶病毒病的关键技术。%In recent years,the tomato yellow leaf curl virus disease in summer was increasingly severe in China.The days of extreme high temperature in summer in northern regions increased,which caused the tomato yellow leaf curl virus disease expanding rapidly and severely damaged the slack season tomato production in summer and autumn.Currently the disease could not effectively controlled,which only be prevented.Using greenhouse tomato in summer and autumn as material,the control effect of field planting with ditch,cooling and moisture retention on tomato yellow leaf curl virus disease was studied.The results showed that when the average maximum temperature in greenhouse was 33-36 ℃,the ground temperature around 5-25 cm of rhizosphere was below 28

  11. The Philippines: Historical Overview.

    Science.gov (United States)

    Shackford, Julie; Aquino, Belinda A., Ed.

    This book provides readings and student lessons about the Philippines. Lessons and activities follow a chronological sequence and provide a good resource for those interested in the Philippines. The materials begin with prehistoric times and continue to the presidency of Corazon Aquino. Each chapter provides background information along with a…

  12. The Philippines: Historical Overview.

    Science.gov (United States)

    Shackford, Julie; Aquino, Belinda A., Ed.

    This book provides readings and student lessons about the Philippines. Lessons and activities follow a chronological sequence and provide a good resource for those interested in the Philippines. The materials begin with prehistoric times and continue to the presidency of Corazon Aquino. Each chapter provides background information along with a…

  13. A New Tomato Hybrid‘Hangza 3’Resistant to Tomato yellow leaf curl virus%抗番茄黄化曲叶病毒番茄新品种‘航杂3号’

    Institute of Scientific and Technical Information of China (English)

    郑积荣; 王慧俐; 王世恒

    2012-01-01

    ‘Hangza 3’is a new red tomato hybrid resistant to Tomato yellow leaf curl virus(TYLCV) and root knot nematode,which is developed by crossing‘HT1-1-3-2-5-3’ב00-03-19’.The hybrid grows strongly with indeterminate plant type and mid-early maturity. Its first inflorescence is 7–8 leaf,Its average single fruit weight is 136 g. The matured fruits are round in shape,red in color with good quality. Especially,it is resistant to TYLCV and root knot nematode and transportation,The average yield of ‘Hangza 3’was 81.0 t · hm-2 as a result of multiple experiments. The hybrid is suitable for cultivation in early spring and late autumn under greenhouse conditions.%‘航杂3号’番茄是以引自荷兰品种‘9956’分离后代的高代自交系‘9956-3-2-1-1-1’经航天搭载诱变后选出的优良自交系‘HT1-1-3-2-5-3’为母本,以引自西班牙品种‘T00’高代自交系‘00-03-19’为父本,杂交育成的新品种。生长势强,无限生长类型,7~8片真叶着生第一花序。果实圆形,单果质量136g,成熟果大红色,耐贮运,商品性好。抗番茄黄化曲叶病毒,兼抗根结线虫病。中早熟,平均产量81.0t·hm-2,适宜保护地早春和秋延后栽培。

  14. H(curl) Auxiliary Mesh Preconditioning

    Energy Technology Data Exchange (ETDEWEB)

    Kolev, T V; Pasciak, J E; Vassilevski, P S

    2006-08-31

    This paper analyzes a two-level preconditioning scheme for H(curl) bilinear forms. The scheme utilizes an auxiliary problem on a related mesh that is more amenable for constructing optimal order multigrid methods. More specifically, we analyze the case when the auxiliary mesh only approximately covers the original domain. The latter assumption is important since it allows for easy construction of nested multilevel spaces on regular auxiliary meshes. Numerical experiments in both two and three space dimensions illustrate the optimal performance of the method.

  15. Computed tomography of ball pythons (Python regius) in curled recumbency.

    Science.gov (United States)

    Hedley, Joanna; Eatwell, Kevin; Schwarz, Tobias

    2014-01-01

    Anesthesia and tube restraint methods are often required for computed tomography (CT) of snakes due to their natural tendency to curl up. However, these restraint methods may cause animal stress. The aim of this study was to determine whether the CT appearance of the lungs differs for ball pythons in a curled position vs. tube restraint. Whole body CT was performed on ten clinically healthy ball pythons, first in curled and then in straight positions restrained in a tube. Curved multiplanar reformatted (MPR) lung images from curled position scans were compared with standard MPR lung images from straight position scans. Lung attenuation and thickness were measured at three locations for each scan. Time for positioning and scanning was 12 ± 5 min shorter for curled snakes compared to tube restraint. Lung parenchyma thickness and attenuation declined from cranial to caudal on both straight and curled position images. Mean lung parenchyma thickness was greater in curled images at locations 1 (P = 0.048) and 3 (P = 0.044). Mean lung parenchyma thickness decreased between location 1 and 2 by 86-87% (straight: curled) and between location 1 and 3 by 51-50% (straight: curled). Mean lung attenuation at location 1 was significantly greater on curled position images than tube restraint images (P = 0.043). Findings indicated that CT evaluation of the lungs is feasible for ball pythons positioned in curled recumbency if curved MPR is available. However, lung parenchyma thickness and attenuation in some locations may vary from those acquired using tube restraint.

  16. 1991 Pinatubo, Philippines Images

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Mount Pinatubo is an andesitic island arc volcano, located on the southwestern part of the island of Luzon in the Philippines. Prior to 1991, it had been dormant for...

  17. 寿光地区感病辣椒TYLCV的分子鉴定和病毒DNA-A序列分析%Molecular Identification and DNA-A Gene Sequence Analysis of Tomato Yellow Leaf Curl Virus on Pepper( Capsicum annuum )in Shouguang Region

    Institute of Scientific and Technical Information of China (English)

    刘春香; 刘彩云; 李会平; 曹京

    2012-01-01

    由于TYLCV的宿主广泛,本试验对山东寿光地区的感病毒病辣椒进行了TYLCV感染的分子鉴定,发现寿光地区的辣椒感染了TYLCV病毒,且感染率较高.通过对病毒基因组DNA-A序列的克隆和序列分析发现,寿光感病辣椒的TYLCV序列与临沂和德州的TYLCV亲缘关系最近,并对具体突变点进行了分析.进化树分析显示,克隆得到的辣椒病毒与墨西哥的TYLCV病毒同源性较高,而与危害辣椒较严重的TYLCSV的遗传距离较远.%Molecular identification were done on tomato yellow leaf curl virus ( TYLCV) isolated from pepper plants showing TYLCV symptoms in Shouguang region because TYLCV have more hosts. The result showed peppers in Shouguang infected TYLCV with high efficiency among detected plants. The isolate was the most close to the TYLCV Linyi isolate and Dezhou isolate, and the detail point mutation of Shouguang TYLCV genome compared with Linyi were analyzed. Phylogenetic tree analysis indicated that TYLCV of mexico were found to show high sequence i-dentity with that we cloned, and with relatively low similarity to TYLCSV strains which damaged pepper seriously.

  18. 侵染云南白肋烟的中国番茄黄化曲叶病毒及伴随卫星DNA分子的基因组特征%Genomic Characterization of DNA-A and Associated Satellite DNA Molecule of an Isolate of Tomato Yellow Leaf Curl China Virus Infecting Nicotiana tabacum White Burley in Yunnan

    Institute of Scientific and Technical Information of China (English)

    李桂新; 范三微; 李正和; 谢艳; 周雪平

    2003-01-01

    从中国云南省大理地区表现曲叶症状的白肋烟(Nicotina tabacum White Burley)上分离到病毒分离物Y43,该病毒可经烟粉虱(Bemisia tabaci)及嫁接传播.用15种粉虱传双生病毒的单抗对病样进行TAS-ELISA检测,结果表明,该病毒属菜豆金色花叶病毒属(Begomovirus)病毒.对DNA-A1.7kb基因组序列测定和分析表明,Y43与中国广西报道的中国番茄黄化曲叶病毒(Tomata yellow leaf curl China virus,TYLCCNV)同源性达89%,其中外壳蛋白(CP)氨基酸同源性达96%,因此Y43应为TYLCCNV的一个新分离物.进一步研究发现,Y43还伴随着一个长1 349 nt的卫星DNA分子(DNAβ).Y43DNAβ与AYVVDNAβ、BYVMVDNAβ和CLCuVDNAβ的同源性较低,而与中国分离的烟草曲叶病毒Y5 DNAβ和Y8 DNA β的亲缘关系较近.DNAβ可能编码7个分子量超过3.5kD的ORF,其中C1推测为有功能的ORF.

  19. Nucleotide sequence and intergeminiviral homologies of the DNA-A of papaya leaf curl geminivirus from India.

    Science.gov (United States)

    Saxena, S; Hallan, V; Singh, B P; Sane, P V

    1998-06-01

    Coat protein gene, rep protein gene and intergenic region of the genome of a whitefly transmitted geminivirus (WTG) causing severe leaf curl in papaya plants were PCR amplified, cloned and sequenced. Comparison of the amino acid sequence of the putative coat protein product of papaya leaf curl virus (PLCV) with some other mono and bipartite WTGs revealed a maximum of 89.8% homology with Indian cassava mosaic virus. The genomic organization of PLCV-India is similar to other WTGs with bipartite genomes. Comparison of the coat protein N-terminal 70 amino acid sequence (and other biological features) of PLCV with other geminiviruses shows that PLCV is a distinct geminivirus from India and is related to WTGs from the old world.

  20. 木尔坦棉花曲叶病毒基因重组和缺失产生DNAβ相关的新型小分子%Novel DNA-β associated molecules produced by sequence recombination and deletion of cotton leaf curl Multan virus complex

    Institute of Scientific and Technical Information of China (English)

    赖艺祯; 谢科; 蔡健和; 秦碧霞; 李战彪; 刘玉乐

    2012-01-01

    [目的]棉花曲叶病是棉花生产上的一种重要的病毒病害,在巴基斯坦和印度等国家地区大面积流行,造成严重的经济损失.近年在中国广西南宁的棉花田间发现了棉花曲叶病害,在广西的黄秋葵中也发生了曲叶病,二者的病原均为木尔坦棉花曲叶病毒(Cotton Leaf Curl Multan Virus,CLCuMV),为了对这2个病害有更深的了解,本文对该双生病毒伴随的DNA小分子进行测序分析.[方法]分别从广西南宁地区感染CLCuMV的3棵棉花和3棵黄秋葵中提取总DNA,用CLCuMV DNAβ的特异引物进行PCR扩增,将产物分离纯化并克隆测序,进行序列比对分析.[结果]从棉花曲叶病害中分离得到了1384 nt的新型重组DNA分子,以及从黄秋葵曲叶病害中分离得到了754 nt的新型缺失型DNA分子.研究结果表明1384 nt重组分子是由CLCuMV GX1的DNA-A和DNAβ重组而成.重组分子大部分来源于CLCuMV的DNA-A,包含基因间隔区,附近的部分AV2和AC1基因,以及反向互补的部分AC3基因.其余部分来源于伴随的DNAβ,包含A-rich区域.分析拼接片段的附近序列,发现接头部分含有2-3个共同碱基,推测为重组作用发生的位点.与以前报道的在实验室中产生的CLCuMV重组分子进行比较显示,DNA-A的基因间隔区和DNAβ的A-rich 区在重组过程中非常保守.另外,754 nt的重组小分子是由CLCuMV Okra1 DNAβ缺失突变产生,缺失了大部分的编码C1蛋白开放阅读框(Open Reading Frame,ORF)以及小部分的A-rich区.[结论]本研究在自然条件下分离到了来源于CLCuMV和卫星DNAβ的重组分子,以及DNAβ缺陷型分子.这2种重组小分子以前未见报道,这也是在中国发现的棉花曲叶病毒中首次发现重组分子.这种基因组变异现象在棉花曲叶病毒的进化和寄主适应过程中可能有重要的意义.%[Objective] Cotton leaf curl disease (CLCuD) is a major constraint to cotton production, causing great economic losses

  1. THE SPORTS SCIENCE OF CURLING: A PRACTICAL REVIEW

    Directory of Open Access Journals (Sweden)

    John L. Bradley

    2009-12-01

    Full Text Available Curling is a sport played on ice in which two teams each deliver 8 granite stones towards a target, or 'house'. It is the only sport in which the trajectory of the projectile can be influenced after it has been released by the athlete. This is achieved by sweeping the ice in front of the stone to change the stone-ice friction and thereby enable to stone to travel further, curl more or stay straight. Hard sweeping is physically demanding. Different techniques of sweeping can also have different effects on the stone. This paper will review the current research behind sweeping a curling stone, outline the physiological demands of sweeping, the associated performance effects and suggest potential strategies of sweeping that can be used by both coaches and curling teams

  2. Collection for Philippines

    CERN Multimedia

    Staff Association

    2015-01-01

    Following the devastating Typhoon Haiyan that hit the Philippines in autumn 2013, a collection of funds to help the victims was organised at CERN. An amount of 16 950 CHF had been contributed and was forwarded to Caritas Switzerland. Tuesday 14 July, we received a message from Caritas in order to thank all the contributors for their generosity and to share with you their project’s progress and the results obtained so far. You can find the report on our website: http://staff-association.web.cern.ch/sites/staff-association.web.cern.ch/files/Docs/Rapport_Philippines.pdf

  3. 耐番茄黄化曲叶病毒病新品种‘申粉V-1’的选育%Breeding of a new tomato variety ‘Shenfen V-1' tolerant to tomato yellow leaf curl virus

    Institute of Scientific and Technical Information of China (English)

    朱龙英; 朱为民; 万延慧; 张辉; 于力; 刘娜

    2012-01-01

    'Shenfen V-1',a new medium-early tomato variety,was bred by crossing between two inbred lines 06-2-4-8-11 (female parent)and Z09A39-3 (male parent). It is of indeterminate growth type,and its first inflorescence occurs at the 7th or 8th node.The mature fruit is pink in color without shoulder,averagely weighs 166.6 g,and is 0.75 kg/cm2 in hardness and tolerant to storage and transportation. The fruit is also good in quality,containing 168.0 mg/kg vitamin C,5.07% soluble solids, and 53.4 mg/kg lycopene.Its yield is 87.00 t/hm2 in spring greenhouse culture and 65.25 t/hm2 in autumn greenhouse culture.It has good tolerance to tomato yellow leaf curl virus (TYLCV)and can be cultivated in areas where TYLCV occurs heavily.%‘申粉V-1’是以自交系06-2-4-8-11为母本、自交系Z09A39-3为父本配制而成的中早熟番茄一代杂种.该品种为无限生长类型,第1花序着生于第7~8节,成熟果粉红色,无绿果肩,单果质量166.6 g,果实硬度0.75 kg/cm2,耐贮运,品质优良,Vc含量168.0 mg/kg,可溶性固形物5.07%,番茄红素53.4mg/kg;春大棚栽培产量87.00 t/hm2,秋大棚栽培产量65.25 t/hm2,耐番茄黄化曲叶病毒病,适于该病发生严重的地区栽培.

  4. Dimensions of Philippine population.

    Science.gov (United States)

    1980-01-01

    Major findings of a 2 1/2 year research program on Philippine population are presented. The population situation is described with respect to fertility, mortality, life expectancy, migration, labor force, and family formation. Policy recommendations addressing problems in each of these areas are made.

  5. Malthus and the Philippines.

    Science.gov (United States)

    Arcega, M B

    1976-01-01

    Jose D. Drilon, Jr., president of Food Terminal, Inc., and a former undersecretary of the then Department of Agriculture and Natural Resources in the Philippines, attributes the widening gap between food supply and demand in developing countries to the high rate of population growth and to the inability of poor countries to produce more food. This situation, in which many countries are facing hunger, was predicted by Thomas Robert Malthus as early as the 16th century. The primary concern of Malthus was the problem of making the food supply keep pace with a constantly growing population. The question arises as to how reliable is Malthusian theory. According to Drilon, Malthus was correct in predicting that population would expand at a rate not previously imagined but that the other aspects of Malthusian theory might not hold true due to the intervention of human beings. For example, it is hoped that the imbalance between population growth and food production can be minimized in the future. In the Philippines there is good reason to be concerned about the validity of Malthusian theory. Although the country's growth rate has been reduced from 3.01% in 1970 to 2.6%, it is still quite high. However, the Philippines has actually been producing sufficient food to feed its population. To make the Philippines self-sufficient in rice, the government initiated the Masagana 99 program in May 1973. Technical and material resources from the public and private sectors were provided to aid rice producers. A nationwide information campaign was also launched to familiarize the farmers with the new methods of rice culture. Masagana 99 has been costly but effective. Since the launching of the program, rice production in the Philippines has been increasing at 7% a year. The government is now using the Masagana 99 formula to increase the production of other crops.

  6. White spot syndrome virus (WSSV) risk factors associated with farming practices in polyculture and monoculture P. monodon farms in the Philippines

    NARCIS (Netherlands)

    Tendencia Alapide, E.; Bosma, R.H.; Verreth, J.A.J.

    2011-01-01

    White spot sydrome virus (WSSV) is one of the most important viral disease of shrimp. Several studies to control the disease have been done. Tank experiments identified WSSV risk factors related to the physico chemical properties of the water. A few studies reported pond level WSSV risk factors. Thi

  7. Trends in Philippine Library History.

    Science.gov (United States)

    Hernandez, Vicente S.

    This paper divides Philippine library history into three periods, establishing a relationship between historical events and library trends. During the Spanish period, modern library trends were introduced through the establishment of the Sociedad Economica in 1780, but did not influence Philippine library culture until the later part of the 19th…

  8. Influence of cutting conditions on chip side curl

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    2004-01-01

    The paper describes the influence of local variations of contact length, cutting speed and material constraint, showing the effect of lubrication, on the side curl of the chip. The following examples are illustrated by experiments: cutting of a tube vs. cutting of a bar; cutting using a tool...

  9. Influence of cutting conditions on chip side curl

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    2004-01-01

    The paper describes the influence of local variations of contact length, cutting speed and material constraint, showing the effect of lubrication, on the side curl of the chip. The following examples are illustrated by experiments: cutting of a tube vs. cutting of a bar; cutting using a tool with...

  10. Computation of Surface Integrals of Curl Vector Fields

    Science.gov (United States)

    Hu, Chenglie

    2007-01-01

    This article presents a way of computing a surface integral when the vector field of the integrand is a curl field. Presented in some advanced calculus textbooks such as [1], the technique, as the author experienced, is simple and applicable. The computation is based on Stokes' theorem in 3-space calculus, and thus provides not only a means to…

  11. Whitefly-mediated transmission of cotton leaf curl Multan betasatellite: evidence for betasatellite encapsidation in coat protein of helper begomoviruses.

    Science.gov (United States)

    Tabein, S; Behjatnia, S A Akbar; Anabestani, A; Izadpanah, K

    2013-01-01

    Cotton leaf curl Multan betasatellite (CLCuMB) is responsible for symptom expression of a devastating disease of cotton in the Indian subcontinent. CLCuMB depends on helper virus replication-associated protein for its replication and on viral coat protein (CP) for its encapsidation. However, no direct evidence of encapsidation of CLCuMB in viral CP has been available. In the present study, non-viruliferous whiteflies were placed on tomato plants that had been agroinoculated with infectious clones of an Iranian isolate of tomato yellow leaf curl virus (TYLCV-[Ab]) and CLCuMB for an acquisition access period of 72 h and then transferred to healthy tomato seedlings at the 3- to 4-leaf stage. Typical symptoms of TYLCV-[Ab] appeared on inoculated seedlings 30-45 days post-inoculation. The presence of TYLCV-[Ab] and CLCuMB DNAs in symptomatic test plants and viruliferous whiteflies was confirmed by PCR analysis using specific primers and DIG Southern blotting. Furthermore, the possibility of CLCuMB DNA encapsidation in TYLCV-[Ab] CP within infected plants was examined by immunocapture PCR. The results showed that CLCuMB DNA was encapsidated in TYLCV-[Ab] CP. Whitefly-mediated transmission of CLCuMB in the presence of helper virus is additional evidence for encapsidation of CLCuMB by TYLCV-[Ab] CP.

  12. Aging trends -- the Philippines.

    Science.gov (United States)

    Biddlecom, A E; Domingo, L J

    1996-03-01

    This report presents a description of the trends in growth of the elderly population in the Philippines and their health, disability, education, work status, income, and family support. The proportion of elderly in the Philippines is much smaller than in other Southeast Asian countries, such as Singapore and Malaysia. The elderly population aged over 65 years increased from 2.7% of total population in 1990 to 3.6% in 1990. The elderly are expected to comprise 7.7% of total population in 2025. The proportion of elderly is small due to the high fertility rate. Life expectancy averages 63.5 years. The aged dependency ratio will double from 5.5 elderly per 100 persons aged 15-64 years in 1990 to 10.5/100 in 2025. A 1984 ASEAN survey found that only 11% of elderly rated their health as bad. The 1990 Census reveals that 3.9% were disabled elderly. Most were deaf, blind, or orthopedically impaired. 16% of elderly in the ASEAN survey reported not seeing a doctor even when they needed to. 54% reported that a doctor was not visited due to the great expense. In 1980, 67% of men and 76% of women aged over 60 years had less than a primary education. The proportion with a secondary education in 2020 is expected to be about 33% for men and 33% for women. 66.5% of men and 28.5% of women aged over 60 years were in the formal labor force in 1990. Women were less likely to receive cash income from current jobs or pensions. 65% of earnings from older rural people was income from agricultural production. 60% of income among urban elderly was from children, and 23% was from pensions. Family support is provided to the elderly in the form of coresidence. In 1988, 68% of elderly aged over 60 years lived with at least one child. Retirement or nursing homes are uncommon. The Philippines Constitution states that families have a duty to care for elderly members.

  13. Epidemiology of Japanese encephalitis in the Philippines: a systematic review.

    Directory of Open Access Journals (Sweden)

    Anna Lena Lopez

    2015-03-01

    Full Text Available Japanese encephalitis virus (JEV is an important cause of encephalitis in most of Asia, with high case fatality rates and often significant neurologic sequelae among survivors. The epidemiology of JE in the Philippines is not well defined. To support consideration of JE vaccine for introduction into the national schedule in the Philippines, we conducted a systematic literature review and summarized JE surveillance data from 2011 to 2014.We conducted searches on Japanese encephalitis and the Philippines in four databases and one library. Data from acute encephalitis syndrome (AES and JE surveillance and from the national reference laboratory from January 2011 to March 2014 were tabulated and mapped.We identified 29 published reports and presentations on JE in the Philippines, including 5 serologic surveys, 18 reports of clinical cases, and 8 animal studies (including two with both clinical cases and animal data. The 18 clinical studies reported 257 cases of laboratory-confirmed JE from 1972 to 2013. JE virus (JEV was the causative agent in 7% to 18% of cases of clinical meningitis and encephalitis combined, and 16% to 40% of clinical encephalitis cases. JE predominantly affected children under 15 years of age and 6% to 7% of cases resulted in death. Surveillance data from January 2011 to March 2014 identified 73 (15% laboratory-confirmed JE cases out of 497 cases tested.This comprehensive review demonstrates the endemicity and extensive geographic range of JE in the Philippines, and supports the use of JE vaccine in the country. Continued and improved surveillance with laboratory confirmation is needed to systematically quantify the burden of JE, to provide information that can guide prioritization of high risk areas in the country and determination of appropriate age and schedule of vaccine introduction, and to measure the impact of preventive measures including immunization against this important public health threat.

  14. Epidemiology of Japanese encephalitis in the Philippines: a systematic review.

    Science.gov (United States)

    Lopez, Anna Lena; Aldaba, Josephine G; Roque, Vito G; Tandoc, Amado O; Sy, Ava Kristy; Espino, Fe Esperanza; DeQuiroz-Castro, Maricel; Jee, Youngmee; Ducusin, Maria Joyce; Fox, Kimberley K

    2015-03-01

    Japanese encephalitis virus (JEV) is an important cause of encephalitis in most of Asia, with high case fatality rates and often significant neurologic sequelae among survivors. The epidemiology of JE in the Philippines is not well defined. To support consideration of JE vaccine for introduction into the national schedule in the Philippines, we conducted a systematic literature review and summarized JE surveillance data from 2011 to 2014. We conducted searches on Japanese encephalitis and the Philippines in four databases and one library. Data from acute encephalitis syndrome (AES) and JE surveillance and from the national reference laboratory from January 2011 to March 2014 were tabulated and mapped. We identified 29 published reports and presentations on JE in the Philippines, including 5 serologic surveys, 18 reports of clinical cases, and 8 animal studies (including two with both clinical cases and animal data). The 18 clinical studies reported 257 cases of laboratory-confirmed JE from 1972 to 2013. JE virus (JEV) was the causative agent in 7% to 18% of cases of clinical meningitis and encephalitis combined, and 16% to 40% of clinical encephalitis cases. JE predominantly affected children under 15 years of age and 6% to 7% of cases resulted in death. Surveillance data from January 2011 to March 2014 identified 73 (15%) laboratory-confirmed JE cases out of 497 cases tested. This comprehensive review demonstrates the endemicity and extensive geographic range of JE in the Philippines, and supports the use of JE vaccine in the country. Continued and improved surveillance with laboratory confirmation is needed to systematically quantify the burden of JE, to provide information that can guide prioritization of high risk areas in the country and determination of appropriate age and schedule of vaccine introduction, and to measure the impact of preventive measures including immunization against this important public health threat.

  15. CRISPR/CAS9: A TOOL TO CIRCUMSCRIBE COTTON LEAF CURL DISEASE

    Directory of Open Access Journals (Sweden)

    Zafar eIqbal

    2016-04-01

    Full Text Available The begomoviruses (family Geminiviridae associated with cotton leaf curl disease (CLCuD pose a major threat to cotton productivity in South-East Asia including Pakistan and India. These viruses have single-stranded, circular DNA genome, of ~2800 nt in size, encapsidated in twinned icosa-hedera, transmitted by ubiquitous whitefly and are associated with satellite molecules referred to as alpha- and betasatellite. To circumvent the proliferation of these viruses numerous techniques, ranging from conventional breeding to molecular approaches have been applied. Such devised strategies worked perfectly well for a short time period and then viruses relapse due to various reasons including multiple infections, where related viruses synergistically interact with each other, virus proliferation and evolution. Another shortcoming is, until now, that all molecular biology approaches are devised to control only helper begomoviruses but not to control associated satellites. Despite the fact that satellites could add various functions to helper begomoviruses, they remain ignored. Such conditions necessitate a very comprehensive technique that can offer best controlling strategy not only against helper begomoviruses but also their associated DNA-satellites. In the current scenario clustered regulatory interspaced short palindromic repeats (CRISPR/CRISPR associated nuclease 9 (Cas9 has proved to be versatile technique that has very recently been deployed successfully to control different geminiviruses. The CRISPR/Cas9 system has been proved to be a comprehensive technique to control different geminiviruses however, like previously used techniques, only a single virus is targeted and hitherto it has not been deployed to control begomovirus complexes associated with DNA-satellites. Here in this article, we proposed an inimitable, unique and broad spectrum controlling method based on multiplexed CRISPR/Cas9 system where a cassette of sgRNA is designed to target

  16. Flat acoustic lens by acoustic grating with curled slits

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Pai; Xiao, Bingmu; Wu, Ying, E-mail: ying.wu@kaust.edu.sa

    2014-10-03

    We design a flat sub-wavelength lens that can focus acoustic wave. We analytically study the transmission through an acoustic grating with curled slits, which can serve as a material with tunable impedance and refractive index for acoustic waves. The effective parameters rely on the geometry of the slits and are independent of frequency. A flat acoustic focusing lens by such acoustic grating with gradient effective refractive index is designed. The focusing effect is clearly observed in simulations and well predicted by the theory. We demonstrate that despite the large impedance mismatch between the acoustic lens and the matrix, the intensity at the focal point is still high due to Fabry–Perot resonance. - Highlights: • Expression of transmission coefficient of an acoustic grating with curled slits. • Non-dispersive and tunable effective medium parameters for the acoustic grating. • A flat acoustic focusing lens with gradient index by using the acoustic grating.

  17. Cosmic Shear of the Microwave Background: The Curl Diagnostic

    CERN Document Server

    Cooray, A R; Caldwell, R R; Cooray, Asantha; Kamionkowski, Marc; Caldwell, Robert R.

    2005-01-01

    Weak-lensing distortions of the cosmic-microwave-background (CMB) temperature and polarization patterns can reveal important clues to the intervening large-scale structure. The effect of lensing is to deflect the primary temperature and polarization signal to slightly different locations on the sky. Deflections due to density fluctuations, gradient-type for the gradient of the projected gravitational potential, give a direct measure of the mass distribution. Curl-type deflections can be induced by, for example, a primordial background of gravitational waves from inflation or by second-order effects related to lensing by density perturbations. Whereas gradient-type deflections are expected to dominate, we show that curl-type deflections can provide a useful test of systematics and serve to indicate the presence of confusing secondary and foreground non-Gaussian signals.

  18. Curling Liquid Crystal Microswimmers: A Cascade of Spontaneous Symmetry Breaking

    Science.gov (United States)

    Krüger, Carsten; Klös, Gunnar; Bahr, Christian; Maass, Corinna C.

    2016-07-01

    We report curling self-propulsion in aqueous emulsions of common mesogenic compounds. Nematic liquid crystal droplets self-propel in a surfactant solution with concentrations above the critical micelle concentration while undergoing micellar solubilization [Herminghaus et al., Soft Matter 10, 7008 (2014)]. We analyzed trajectories both in a Hele-Shaw geometry and in a 3D setup at variable buoyancy. The coupling between the nematic director field and the convective flow inside the droplet leads to a second symmetry breaking which gives rise to curling motion in 2D. This is demonstrated through a reversible transition to nonhelical persistent swimming by heating to the isotropic phase. Furthermore, autochemotaxis can spontaneously break the inversion symmetry, leading to helical trajectories in 3D.

  19. Philippines revises power plans

    Energy Technology Data Exchange (ETDEWEB)

    Hayes, D.

    1988-02-01

    Following an unexpected surge in electricity demand the Philippines has revised its medium term power development programme. The sharp increase in electricity consumption follows three years of slack demand due to civil disturbances before the end of the Macros administration and the election of Corazon Aquino as President in 1986. Originally, the Aquino government's medium term power development plans called for about 500MW of generating capacity to be installed between 1986 and 1992. The three proposed plants were scheduled for commissioning in 1991 and 1992. However, a spurt in power demand growth during the past nine months has caused concern among industrialists that power shortages could occur by the end of the decade. So additional capacity will be installed to prevent an anticipated shortfall in electricity supplies by the early 1990s.

  20. Effect of neck flexion restriction on sternocleidomastoid and abdominal muscle activity during curl-up exercises.

    Science.gov (United States)

    Lee, Dong-Kyu; Moon, Dong-Chul; Hong, Ki-Hoon

    2016-01-01

    [Purpose] The purpose of this study was to investigate the effect of neck flexion restriction on sternocleidomastoid (SCM), rectus abdominis (RA), and external oblique (EO) muscle activity during a traditional curl-up exercise and a curl-up with neck flexion restriction. [Subjects] In total, 13 healthy male subjects volunteered for this study. [Methods] All subjects performed a traditional curl-up exercise and a curl-up exercise in which neck flexion was restricted by the subject's hand. Surface electromyography (EMG) signals were recorded from the SCM, RA, and EO during the curl-up. [Results] There was significantly lower EMG activity of the SCM during the curl-up exercise with neck flexion restriction compared to the traditional curl-up exercise. Conversely, the activity of the RA and EO muscles was significantly higher in the curl-up exercise with neck flexion restriction than in the traditional curl-up exercise. [Conclusion] Neck flexion restriction is recommended to prevent excessive activation of superficial cervical flexors during the curl-up exercise.

  1. Analytical investigation into the resonance frequencies of a curling probe

    Science.gov (United States)

    Arshadi, Ali; Brinkmann, Ralf Peter

    2016-08-01

    The term ‘active plasma resonance spectroscopy’ (APRS) denotes a class of closely related plasma diagnostic methods which utilize the natural ability of plasmas to resonate on or near the electron plasma frequency {ω\\text{pe}} ; an electrical radio frequency signal (in the GHz range) is coupled into the plasma via an antenna or a probe, the spectral response is recorded and a mathematical model is employed to determine plasma parameters such as the plasma density and the electron temperature. The curling probe, recently invented by Liang et al (2011 Appl. Phys. Express 4 066101), is a novel realization of the APRS concept which has many practical advantages. In particular, it can be miniaturized and flatly embedded into the chamber wall, thus allowing the monitoring of plasma processes without contamination nor disturbance. Physically, the curling probe can be understood as a ‘coiled’ form of the hairpin probe (Stenzel 1976 Rev. Sci. Instrum. 47 603). Assuming that the spiralization of the probe has little electrical effect, this paper investigates the characteristcs of a ‘straightened’ curling probe by modeling it as an infinite slot-type resonator that is in direct contact with the plasma. The diffraction of an incident plane wave at the slot is calculated by solving the cold plasma model and Maxwell’s equations simultaneously. The resonance frequencies of the probe are derived and are found to be in good agreement with the numerical results of the probe inventors.

  2. Hydro-responsive curling of the resurrection plant Selaginella lepidophylla.

    Science.gov (United States)

    Rafsanjani, Ahmad; Brulé, Véronique; Western, Tamara L; Pasini, Damiano

    2015-01-27

    The spirally arranged stems of the spikemoss Selaginella lepidophylla, an ancient resurrection plant, compactly curl into a nest-ball shape upon dehydration. Due to its spiral phyllotaxy, older outer stems on the plant interlace and envelope the younger inner stems forming the plant centre. Stem curling is a morphological mechanism that limits photoinhibitory and thermal damages the plant might experience in arid environments. Here, we investigate the distinct conformational changes of outer and inner stems of S. lepidophylla triggered by dehydration. Outer stems bend into circular rings in a relatively short period of desiccation, whereas inner stems curl slowly into spirals due to hydro-actuated strain gradient along their length. This arrangement eases both the tight packing of the plant during desiccation and its fast opening upon rehydration. The insights gained from this work shed light on the hydro-responsive movements in plants and might contribute to the development of deployable structures with remarkable shape transformations in response to environmental stimuli.

  3. The CURL-CoFoR Collaborative Collection Management Project

    Directory of Open Access Journals (Sweden)

    Gregory Walker

    2005-09-01

    Full Text Available CoFoR originated from an initiative by CURL, the Consortium of Research Libraries in the British Isles. CURL’s Task Force on Resource Management proposed a project, which would develop and test several interrelated aspects of collaborative collection management (CCM, to help in determining the most effective kinds of collaboration between major research collections. The Task Force was concerned that local pressures on libraries were tending to make them take collection management decisions in isolation, and they were looking for a framework within which better informed decisions on libraries’ collections could be taken with greater confidence and a clearer awareness of national provision. CURL was seeking to establish which CCM approaches could be expected to: · offer the most attractive benefits for costs incurred; · be applicable to a wide range of subjects / areas; · and be viable in the longer term. In lat 2002 CURL accepted a proposal from an existing project team to undertake this work.

  4. Molecular Evolution of Enterovirus 68 Detected in the Philippines

    Science.gov (United States)

    Imamura, Tadatsugu; Suzuki, Akira; Lupisan, Socorro; Okamoto, Michiko; Aniceto, Rapunzel; Egos, Rutchie J.; Daya, Edgardo E.; Tamaki, Raita; Saito, Mariko; Fuji, Naoko; Roy, Chandra Nath; Opinion, Jaime M.; Santo, Arlene V.; Macalalad, Noel G.; Tandoc, Amado; Sombrero, Lydia; Olveda, Remigio; Oshitani, Hitoshi

    2013-01-01

    Background Detection of Enterovirus 68 (EV68) has recently been increased. However, underlying evolutionary mechanism of this increasing trend is not fully understood. Methods Nasopharyngeal swabs were collected from 5,240 patients with acute respiratory infections in the Philippines from June 2009 to December 2011. EV68 was detected by polymerase chain reaction (PCR) targeting for 5′ untranslated region (5′UTR), viral protein 1 (VP1), and VP4/VP2. Phylogenetic trees were generated using the obtained sequences. Results Of the 5,240 tested samples, 12 EV68 positive cases were detected between August and December in 2011 (detection rate, 0.23%). The detection rate was higher among inpatients than outpatients (p<0.0001). Among VP1 sequences detected from 7 patients in 2011, 5 in lineage 2 were diverged from those detected in the Philippines in 2008, however, 2 in lineage 3 were not diverged from strains detected in the Philippines in 2008 but closely associated with strains detected in the United States. Combined with our previous report, EV68 occurrences were observed twice in the Philippines within the last four years. Conclusions EV68 detections might be occurring in cyclic patterns, and viruses might have been maintained in the community while some strains might have been newly introduced. PMID:24073203

  5. [Researches on virology at the Tohoku University Research Center in the Philippines].

    Science.gov (United States)

    Oshitani, Hitoshi; Saito, Mariko; Okamoto, Michiko; Tamaki, Raita; Kamigaki, Taro; Suzuki, Akira

    2013-01-01

    Tohoku University Graduate School of Medicine has established the Tohoku-RITM Collaborative Research Center on Emerging and Re-emerging Diseases at Research Institute for Tropical Medicine (RITM) in the Philippines in 2008. Our aim of the center is to conduct operational researches, which can contribute to control of infectious diseases in the Philippines. Therefore most of our researches in the Philippines are being conducted in the fields. Main research themes include severe acute respiratory infections in children, influenza disease burden study, molecular epidemiology of rabies, and viral etiology of acute diarrhea. The study on severe acute respiratory infections in children in Leyte Island has recruited hospitalized cases with severe pneumonia. We showed that enterovirus 68 was one of important causative agents in severe pneumonia cases. We also conducted other analyses including molecular epidemiology of respiratory syncytial virus (RSV) and pathogenesis of human rhinoviruses (HRV). Based on these studies, we initiated more comprehensive researches in the Philippines since 2010.

  6. Curl-meter of Electrical Fields In The Ground.

    Science.gov (United States)

    Krylov, S. M.; Maibuk, Z.-Ju. Ja.; Nikiforova, N. N.

    A special instrument U curl-meter was designed and manufactured in the Institute of Physics of the Earth of RAS for measuring of variable electric fields during alternation of stressedly-deformed state in rock mass. The instrument consist the four-electrode unit and a circuit of analogue signal processing for separation of E U circulations or according to the StokesSs theorem, Curl E in absence of indirect sources. Four electrodes are laied out in rocks on angles of square and they are affixed by ring-type circuit to uninverting inputs of precision operational amplifiers. First input is connected to electrode N1, the second one is connected to N2 and so on. The independent inputs are grounded to a arbitrary point (the fifth electrode is SzeroT). The transmission factors of the circuit are set by resistors accurate to within 0.25 %. First and third, and also second and fourth outputs of the amplifiers are connected to the grad EX and grad EY calculation circuit (deduction circuits). So, if the vector components have different signs of both two EX values and two values EY, the gradient calculation circuit generates signal extremums. If in this case the signs inside pairs are identical , that means that the signal not- ring-type and it is absent on output (difference of the equal values with equal signs). The signals from outputs of the gradient calculations act into adding device for calculation of Curl E (circulation). Curl-meter differs by high security from clutters and from cues on any of inputs rather of "zero point" (ground) reacting only on a ring-type current, thus it is essential (on the order) the noise level and drift of operational amplifiers is moderated. Curl-meter works in a complex of measuring devices on Obninsk seismological polygon for study of behavior of superlow frequency of tectonic genesis electromagnetic emission. Through four inputs (electrode spacing 7x7 2, resistance between welding rods 0.8 - 1.1 kOm), manufactured from fine- dyspersated

  7. China expreses interest in philippine hydro

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    MEMBERS OF A CHINESE trade mission which visited the Philippines in December 2000 have said they are prepared to invest up to US$ 19M in the Philippines' energy sector, and are particularly interested in hydro power projects.

  8. PARALLEL AUXILIARY SPACE AMG FOR H(curl) PROBLEMS

    Institute of Scientific and Technical Information of China (English)

    Tzanio V. Kolev; Panayot S. Vassilevski

    2009-01-01

    In this paper we review a number of auxiliary space based preconditioners for the second order definite and semi-definite Maxwell problems discretized with the lowest order Nedelec finite elements. We discuss the parallel implementation of the most promising of these methods, the ones derived from the recent Hiptmair-Xu (HX) auxiliary space decomposition [Hiptmair and Xu, SIAM J. Numer. Anal., 45 (2007), pp. 2483-2509]. An extensive set of numerical experiments demonstrate the scalability of our implementation on large-scale H(curl) problems.

  9. Lymphatic filariasis in the Philippines.

    Science.gov (United States)

    Kron, M; Walker, E; Hernandez, L; Torres, E; Libranda-Ramirez, B

    2000-08-01

    Lymphatic filariasis caused by Wuchereria bancrofti and Brugia malayi is endemic throughout most of the southern half of the Philippine archipelago. Economic and manpower shortages prior to 1996 made it difficult to acquire new prevalence data and vector control data concurrently from all provinces. Nevertheless, analysis of cumulative prevalence data on filariasis indicates the persistence of filariasis in each of the three major island groups - Luzon, Visayas and Mindanao - including 45 out of 77 provinces. Here, Michael Kron and colleagues summarize the prevalence data, and review host, parasite and vector characteristics relevant to the design and implementation of disease control initiatives in the Philippines planned for the year 2000.

  10. Edge curling that has plagued scrolls for millenniums

    Science.gov (United States)

    Chou, Ming-Han; Shen, Wei-Chao; Wang, Yi-Ping; Hung, Sun-Hsin; Hong, Tzay-Ming; Department of Registration and Conservation, National Palace Museum Collaboration; Department of Physics, National Tsing Hua University Team

    2014-03-01

    Qi-Wa refers to the up curl on the lengths of handscrolls and hanging scrolls, which has troubled Chinese artisans and emperors for as long as the art of painting and calligraphy exists. This warp is unwelcome not only for aesthetic reasons, but its potential damage to the fiber and ink. Although it is generally treated as a part of the cockling and curling due to moisture, consistency of paste, and defects from the mounting procedures, we demonstrate that the spontaneous extrinsic curvature incurred from the storage is in fact more essential to understanding and curing Qi-Wa. In contrast to the former factors whose effects are less predictable, the plastic deformation and strain distribution on a membrane are a well-defined mechanical problem. We study this phenomenon by experiments, theoretical models, and Molecular Dynamics Simulation, and obtain consistent scaling relations for the Qi-Wa height. This knowledge enables us to propose modifications on the traditional mounting techniques, that are tested on real mounted paper to be effective at mitigating Qi-Wa. By experimenting on polymer-based films, we demonstrate possible relevance of our study to the modern development of flexible electronic paper.

  11. Curling Edges: A Problem that Has Plagued Scrolls for Millennia

    Science.gov (United States)

    Chou, Ming-Han; Shen, Wei-Chao; Wang, Yi-Ping; Hung, Sun-Hsin; Hong, Tzay-Ming

    2014-01-01

    Qi-Wa refers to the up curl on the lengths of hand scrolls and hanging scrolls, which has troubled Chinese artisans and emperors for as long as the art of painting and calligraphy has existed. This warp is unwelcome not only for aesthetic reasons, but its potential damage to the fiber and ink. Although it is generally treated as a part of the cockling and curling due to moisture, consistency of paste, and defects from the mounting procedures, we demonstrate that the spontaneous extrinsic curvature incurred from the storage is in fact more essential to understanding and curing Qi-Wa. In contrast to the former factors whose effects are less predictable, the plastic deformation and strain distribution on a membrane are a well-defined mechanical problem. We study this phenomenon by experiments, theoretical models, and molecular dynamics simulation, and obtain consistent scaling relations for the Qi-Wa height. This knowledge enables us to propose modifications on the traditional mounting techniques that are tested on real mounted paper to be effective at mitigating Qi-Wa. By experimenting on polymer-based films, we demonstrate the possible relevance of our study to the modern development of flexible electronic paper.

  12. College Choice in the Philippines

    Science.gov (United States)

    Tan, Christine Joy

    2009-01-01

    This descriptive and correlational study examined the applicability of major U.S. college choice factors to Philippine high school seniors. A sample of 226 students from a private school in Manila completed the College Choice Survey for High School Seniors. Cronbach's alpha for the survey composite index was 0.933. The purposes of this…

  13. Philippine Friendship Delegation in China

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    <正>Avisit by a Philippines delegation to major Chinese cities last October provided an opportunity for some longstanding friends of China, some preceding the establishment of diplomatic relations,to see and appreciate the great changes the country has undergone in recent decades. They were greatly impressed and full of praise for the many obvious achievements.

  14. Scientists Investigate Recent Philippine Landslide

    Science.gov (United States)

    Lagmay, Alfredo Mahar A.; Ong, John Burtkenley T.; Fernandez, Dan Ferdinand D.; Lapus, Mark R.; Rodolfo, Raymond S.; Tengonciang, Arlene Mae P.; Soria, Janneli Lea A.; Baliatan, Eden G.; Quimba, Zareth L.; Uichanco, Christopher L.; Paguican, Engielle Mae R.; Remedio, Armelle Reca C.; Lorenzo, Genevieve Rose H.; Avila, Francia B.; Valdivia, Waldemar

    2006-03-01

    A massive landslide devastated the community of Barangay Guinsaugon, Municipality of St. Bernard, Southern Leyte Province, Philippines, at about 10:30 local time on 17 February. The landslide occurred along the steep fault scarp of the Philippine Fault Zone (PFZ) (Figure 1a), a large and active tectonic structure that traverses the entire length of the Philippines [Allen, 1962]. Barangay Guinsaugon is located at the foot of the scarp, directly in the path of the downward moving mass of earth. As of 24 February, the landslide caused 122 confirmed deaths; 1,328 people still are missing. To assist in the search and rescue operations that followed the landside, a team of geologists and physicists from the University of Philippines (UP-Diliman, Quezon City) and Ateneo de Manila University conducted an investigation of this area on 21-25 February. The UP-Ateneo team provided technical advice on the geology, which included the identification of the type and characteristics of the landslide.

  15. CPI buildup in the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    Ronquillo, B.

    1980-02-11

    To slash oil-import bills, the Philippines is pushing hard to set up about 35 distilleries to produce ethyl alcohol which will be blended with gasoline. The project, which will use sugarcane as the feedstock may result in ethylene being produced eventually from ethanol. This would make the nation less dependent on petroleum as the basic source for chemicals.

  16. CPAFFC Delegation in the Philippines

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    <正>The signing of a cooperative agreement on comprehensive cooperation was one of the main fruits of a visit made to the Philippines Feb. 28-March 3 by a goodwill delegation led by Luo Qin,Secretary General of the CPAFFC. The participants in the program are the China Service Center for Friendship and Cooperation with For-

  17. Cotton Leaf Curl Multan Betasatellite DNA as a Tool to Deliver and Express the Human B-Cell Lymphoma 2 (Bcl-2) Gene in Plants.

    Science.gov (United States)

    Kharazmi, Sara; Ataie Kachoie, Elham; Behjatnia, Seyed Ali Akbar

    2016-05-01

    The betasatellite DNA associated with Cotton leaf curl Multan virus (CLCuMB) contains a single complementary-sense ORF, βC1, which is a pathogenicity determinant. CLCuMB was able to replicate in plants in the presence of diverse helper geminiviruses, including Tomato leaf curl virus-Australia (TLCV-Au), Iranian isolate of Tomato yellow leaf curl virus (TYLCV-[Ab]), and Beet curly top virus (BCTV-Svr), and can be used as a plant gene delivery vector. To test the hypothesis that CLCuMB has the potential to act as an animal gene delivery vector, a specific insertion construct was produced by the introduction of a human B-cell lymphoma 2 (Bcl-2) cDNA into a mutant DNA of CLCuMB in which the βC1 was deleted (β∆C1). The recombinant βΔC1-Bcl-2 construct was successfully replicated in tomato and tobacco plants in the presence of TLCV-Au, BCTV-Svr and TYLCV-[Ab]. Real-time PCR and Western blot analyses of plants containing the replicative forms of recombinant βΔC1-Bcl-2 DNA showed that Bcl-2 gene was expressed in an acceptable level in these plants, indicating that β∆C1 can be used as a tool to deliver and express animal genes in plants. This CLCuMB-based system, having its own promoter activity, offers the possibility of production of animal recombinant proteins in plants.

  18. Flat acoustic lens by acoustic grating with curled slits

    KAUST Repository

    Peng, Pai

    2014-10-01

    We design a flat sub-wavelength lens that can focus acoustic wave. We analytically study the transmission through an acoustic grating with curled slits, which can serve as a material with tunable impedance and refractive index for acoustic waves. The effective parameters rely on the geometry of the slits and are independent of frequency. A flat acoustic focusing lens by such acoustic grating with gradient effective refractive index is designed. The focusing effect is clearly observed in simulations and well predicted by the theory. We demonstrate that despite the large impedance mismatch between the acoustic lens and the matrix, the intensity at the focal point is still high due to Fabry-Perot resonance.

  19. PHILIPPINE COCONUT INDUSTRY AND THE INTERNATIONAL TRADE

    OpenAIRE

    Oniki, Shunji

    1992-01-01

    This study explores effects of Philippines' coconut policies on the performance in the international market. Analysis of the coconut sector found that the Philippine government successfully changed the structure of the coconut industry during the 1970's using a fund collected as coconut levies. Since the Philippines dominated the international trade market of coconut products, it could exercise dominant market power in the world trade, by integrating the domestic sector. However, the industri...

  20. Philippines - Revenue Administration Reform Project (RARP) Evaluation

    Data.gov (United States)

    Millennium Challenge Corporation — The Millennium Challenge Account-Philippines' (MCA-P) implementation of the Revenue Administration Reform Project (RARP) is expected to improve tax administration,...

  1. Combating opposition in the Philippines.

    Science.gov (United States)

    Marcelino, A B

    1996-01-01

    The Philippine family planning movement has been in existence for 25 years. The Catholic Church, however, has launched a smear campaign against it with the shibboleths of antichild, antifamily, antilife, and pro-abortion that had to be countered by the association. At the Pro-Life's First Training Congress on Love, Life, and Family held in 1995 at the country's oldest Catholic university in Manila (University of Santo Tomas), charges were leveled against it and the International Planned Parenthood Federation as owners of multinational companies that manufacture infant formulas, contraceptives, and abortion machines. At the IPPF Members' Assembly in 1995 this anti-family planning campaign escalated with charges that the IPPF was the agent of free sex, promiscuity, and low morality. All this in the face of IPPF's strong commitment to reproductive rights embodied in the Cairo and Beijing conference concluding documents on reproductive health. The President of the Philippines also addressed this meeting and voiced his support for family planning as an integral part of national development. The major issues in this arena are the gender relations between men and women; prevailing cultural norms and beliefs; lack of information and education; inadequate health care delivery; and poverty. In this climate Pro-Life, Philippines and Human Life International are outspoken in their 1996-98 advocacy campaign to: 1) capture key local government positions and promote their anti-FP agenda; 2) to undertake grassroots education through the Church's Family Life Apostolate; and 3) to campaign for the removal of population education currently being introduced in public schools and replace them with anti-FP and anti-population control education modules. This campaign poses a major challenge for the Philippine FP organization to work toward: 1) changing the knowledge, attitudes, and practices of women and couples toward FP; 2) changing the knowledge, attitudes, and practices of decision

  2. Characterization of a New World Monopartite Begomovirus Causing Leaf Curl Disease of Tomato in Ecuador and Peru Reveals a New Direction in Geminivirus Evolution

    OpenAIRE

    Melgarejo, Tomas A.; Kon, Tatsuya; Rojas, Maria R.; Paz-Carrasco, Lenin; Zerbini, F. Murilo; Gilbertson, Robert L.

    2013-01-01

    All characterized whitefly-transmitted geminiviruses (begomoviruses) with origins in the New World (NW) have bipartite genomes composed of a DNA-A and DNA-B component. Recently, an NW begomovirus lacking a DNA-B component was associated with tomato leaf curl disease (ToLCD) in Peru, and it was named Tomato leaf deformation virus (ToLDeV). Here, we show that isolates of ToLDeV associated with ToLCD in Ecuador and Peru have a single, genetically diverse genomic DNA that is most closely related ...

  3. Wind Stress, METOP ASCAT, 0.25 degrees, Global, Near Real Time, Curl

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes near real time wind stress data in zonal, meridional, modulus, and wind stress curl sets. This data begins with wind velocity...

  4. A Scott bench with ergonomic thorax stabilisation pad improves body posture during preacher arm curl exercise.

    Science.gov (United States)

    Biscarini, Andrea; Benvenuti, Paolo; Busti, Daniele; Zanuso, Silvano

    2016-05-01

    We assessed whether the use of an ergonomic thorax stabilisation pad, during the preacher arm curl exercise, could significantly reduce the excessive shoulder protraction and thoracic kyphosis induced by the standard flat pad built into the existing preacher arm curl equipment. A 3D motion capture system and inclinometers were used to measure shoulder protraction and thoracic kyphosis in 15 subjects performing preacher arm curl with a plate-loaded machine provided with the standard flat pad. The same measures were repeated after replacing the flat pad with a new ergonomic pad, specifically designed to accommodate the thorax profile and improve body posture. Pad replacement significantly (p thorax stabilisation pad for the preacher arm curl exercise. The new ergonomic pad improves the poor posture conditions induced by the standard flat pad and may potentially allow a more effective training, prevent musculoskeletal discomfort, improve the breathing function and reduce the risk of injury.

  5. Wind Stress, QuikSCAT SeaWinds, 0.25 degrees, Global, Science Quality, Curl

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes science quality wind stress data in zonal, meridional, modulus, and wind stress curl sets. This data begins with wind velocity...

  6. Wind Stress, METOP ASCAT, 0.25 degrees, Global, Near Real Time, Curl

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes near real time wind stress data in zonal, meridional, modulus, and wind stress curl sets. This data begins with wind velocity...

  7. Wind Stress, QuikSCAT SeaWinds, 0.25 degrees, Global, Science Quality, Curl

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes science quality wind stress data in zonal, meridional, modulus, and wind stress curl sets. This data begins with wind velocity...

  8. Scales of North Atlantic wind stress curl determined from the comprehensive ocean-atmosphere data set

    Science.gov (United States)

    Ehret, Laura L.; O'Brien, James J.

    1989-01-01

    Nineteen years of wind data over the North Atlantic are used to calculate a field of wind stress curl. An empirical orthogonal function (EOF) analysis is performed on this field, resulting in spatial patterns of wind stress curl and associated time series. A Monte Carlo technique is used to establish the statistical significance of each spatial pattern, and the associated time series are spectrally analyzed. The first four statistically significant EOF modes represent more than 50 percent of the curl variance, and the spatial patterns of curl associated with these modes exhibit the major elements of North Atlantic climatology. Most of the time series spectral variance is contained in annual and semiannual frequencies. The features observed include the individual annual variation of the subtropical high and the subpolar low, the annual oscillation of intensity between pressure centers, the influence of localized strong SST gradients and associated cyclogenesis regions, and the constant nature of the trades.

  9. Drama-in-Schools in the Philippines

    Science.gov (United States)

    Pañares, Alice A.; Cabangon, Maria Gloriosa S.

    2016-01-01

    Drama in the Philippines has been an integral part of the lives of Filipinos. Drama-in-schools came about with the establishment of the formal school system during the Spanish and American period of colonisation of the Philippines. With the establishment of the public schools system, the American teachers introduced drama in the schools, as part…

  10. Case Study: Operation Enduring Freedom Philippines

    Science.gov (United States)

    2010-10-01

    del Norte on Mindanao. Much of the Armed Forces of the Philippines action on Basilan was curtailed to pursue the ASG leader, Abu Sabaya, who was...importance of space, and governed space, and ungoverned space. What we’ve seen in the Southern Philippines we’ve seen in places like the Horn of Africa

  11. Sustainable Landscapes Assessment for the Philippines

    Science.gov (United States)

    Oliver Agoncillo; Jennifer Conje; Susan Cordell; James Halperin; Roopa Karia; Beth. Lebow

    2011-01-01

    As part of their development of a new Country Development Strategy, and with potential for incoming Sustainable Landscapes funding, USAID/Philippines commissioned the U.S. Forest Service to work with them on an assessment of efforts aimed at Reducing Emissions from Deforestation and Forest Degradation (REDD+) in the Philippines, including the role of conservation,...

  12. The Philippine "Hip Hop Stick Dance"

    Science.gov (United States)

    Lewis, Lisa

    2012-01-01

    This article introduces a dance that blends the traditional cultural heritage of the Philippines with modern music and moves. "Hip Hop Stick Dance" incorporates Tinikling (the Philippine national dance) and Arnis (a Filipino style of martial arts) to create a contemporary combination of rhythm, dance, and fitness. It was designed to introduce…

  13. Lexicography in the Philippines (1600-1800)

    NARCIS (Netherlands)

    Fernández Rodriguez, R.

    2014-01-01

    Spanish missionary lexicography in America and the Philippines is extensive and deserving of detailed research. In the Philippines, from 1600 up to 1898, more than fifty vocabularies were published in thirteen different languages. Alongside these are numerous vocabularies preserved only as manuscrip

  14. The Philippine "Hip Hop Stick Dance"

    Science.gov (United States)

    Lewis, Lisa

    2012-01-01

    This article introduces a dance that blends the traditional cultural heritage of the Philippines with modern music and moves. "Hip Hop Stick Dance" incorporates Tinikling (the Philippine national dance) and Arnis (a Filipino style of martial arts) to create a contemporary combination of rhythm, dance, and fitness. It was designed to introduce…

  15. Lexicography in the Philippines (1600-1800)

    NARCIS (Netherlands)

    R. Fernández Rodriguez

    2014-01-01

    Spanish missionary lexicography in America and the Philippines is extensive and deserving of detailed research. In the Philippines, from 1600 up to 1898, more than fifty vocabularies were published in thirteen different languages. Alongside these are numerous vocabularies preserved only as manuscrip

  16. Curling dynamics of naturally curved ribbons from high to low Reynolds numbers

    Science.gov (United States)

    Albarran Arriagada, Octavio; Massiera, Gladys; Abkarian, Manouk

    2012-11-01

    Curling deformation of thin elastic sheets appears in numerous structures in nature, such as membranes of red blood cells, epithelial tissues or green algae colonies to cite just a few examples. However, despite its ubiquity, the dynamics of curling propagation in a naturally curved material remains still poorly investigated. Here, we present a coupled experimental and theoretical study of the dynamical curling deformation of naturally curved ribbons. Using thermoplastic and metallic ribbons molded on cylinders of different radii, we tune separately the natural curvature and the geometry to study curling dynamics in air, water and in viscous oils, thus spanning a wide range of Reynolds numbers. Our theoretical and experimental approaches separate the role of elasticity, gravity and hydrodynamic dissipation from inertia and emphasize the fundamental differences between the curling of a naturally curved ribbon and a rod described by the classical Elastica. Our work shows evidence for the propagation of a single instability front, selected by a local buckling condition. We show that depending on gravity, and both the Reynolds and the Cauchy numbers, the curling speed and shape are modified by the large scale drag and the local lubrication forces. This work was supported by the French Ministry of Research, the CNRS Physics-Chemistry-Biology Interdisciplinary Pro- gram, the University Montpellier 2 Interdisciplinary Program and the Region Languedoc-Roussillon.

  17. Finding the Missing Link to a Successful Philippine Counterinsurgency Strategy

    Science.gov (United States)

    2007-06-01

    Globalization and State Capacity: The Philippines,” Philippine Institute for Development Studies, ( Discussion Paper Series No. 2003-20, Dec 2003...Capacity: The Philippines.” Philippine Institute for Development Studies. Discussion Paper Series No. 2003-20, Dec 2003. Available from http

  18. Analytical solutions of the electrostatically actuated curled beam problem

    KAUST Repository

    Younis, Mohammad I.

    2014-07-24

    This works presents analytical expressions of the electrostatically actuated initially deformed cantilever beam problem. The formulation is based on the continuous Euler-Bernoulli beam model combined with a single-mode Galerkin approximation. We derive simple analytical expressions for two commonly observed deformed beams configurations: the curled and tilted configurations. The derived analytical formulas are validated by comparing their results to experimental data and numerical results of a multi-mode reduced order model. The derived expressions do not involve any complicated integrals or complex terms and can be conveniently used by designers for quick, yet accurate, estimations. The formulas are found to yield accurate results for most commonly encountered microbeams of initial tip deflections of few microns. For largely deformed beams, we found that these formulas yield less accurate results due to the limitations of the single-mode approximation. In such cases, multi-mode reduced order models are shown to yield accurate results. © 2014 Springer-Verlag Berlin Heidelberg.

  19. Cotton leaf curl disease - an emerging threat to cotton production worldwide.

    Science.gov (United States)

    Sattar, M Naeem; Kvarnheden, Anders; Saeed, Muhammad; Briddon, Rob W

    2013-04-01

    Cotton leaf curl disease (CLCuD) is a serious disease of cotton which has characteristic symptoms, the most unusual of which is the formation of leaf-like enations on the undersides of leaves. The disease is caused by whitefly-transmitted geminiviruses (family Geminiviridae, genus Begomovirus) in association with specific, symptom-modulating satellites (betasatellites) and an evolutionarily distinct group of satellite-like molecules known as alphasatellites. CLCuD occurs across Africa as well as in Pakistan and north-western India. Over the past 25 years, Pakistan and India have experienced two epidemics of the disease, the most recent of which involved a virus and satellite that are resistance breaking. Loss of this conventional host-plant resistance, which saved the cotton growers from ruin in the late 1990s, leaves farmers with only relatively poor host plant tolerance to counter the extensive losses the disease causes. There has always been the fear that CLCuD could spread from the relatively limited geographical range it encompasses at present to other cotton-growing areas of the world where, although the disease is not present, the environmental conditions are suitable for its establishment and the whitefly vector occurs. Unfortunately recent events have shown this fear to be well founded, with CLCuD making its first appearance in China. Here, we outline recent advances made in understanding the molecular biology of the components of the disease complex, their interactions with host plants, as well as efforts being made to control CLCuD.

  20. Contraceptive practice in the Philippines.

    Science.gov (United States)

    1989-01-01

    Contraceptive use increased substantially in the Philippines from 1983 to 1986. Prevalence increased 32.1% in 1983, 45.7% in 1986. Increase in use was among married women in Northern Mindanao, Central Visayas, Southern Tagalog, Bicol, and Western Mindanao. Prevalence remained unchanged in Southern Mindanao. During this period, modern contraceptive methods also increased with more women opting to use the pill, IUD, injectables, and sterilization. In most areas in the Philippines, contraceptive use escalated with the more traditional methods like rhythm and withdrawal. Modern family planning increased most in urban areas such as Northern and Western Mindanao. Age also played a significant part in the study. Women aged 20-24 increased usage between 1983 and 1986. A more substantial increase was noticed in the 25-49 year old group, and the highest increase came from married women aged 30-34, with at least 3-4 children, during 1983. This figure shifted in 1986 with contraceptive use becoming more pronounced among 35-39 year olds. Modern methods declined at the 40-44 age bracket due to low fecundity. Throughout the country, modern contraceptive methods were more widely practiced by working women and was defined occupationally. Women in production, labor, and service occupations displayed a low level of modern usage, while those women in professional, administrative, and technical positions were more likely to use modern methods.

  1. EFFECT OF THE SHOULDER POSITION ON THE BICEPS BRACHII EMG IN DIFFERENT DUMBBELL CURLS

    Directory of Open Access Journals (Sweden)

    Taian M.M. Vieira

    2009-03-01

    Full Text Available Incline Dumbbell Curl (IDC and Dumbbell Preacher Curl (DPC are two variations of the standard Dumbbell Biceps Curl (DBC, generally applied to optimize biceps brachii contribution for elbow flexion by fixing shoulder at a specific angle. The aim of this study is to identify changes in the neuromuscular activity of biceps brachii long head for IDC, DPC and DBC exercises, by taking into account the changes in load moment arm and muscle length elicited by each dumbbell curl protocol. A single cycle (concentric-eccentric of DBC, IDC and DPC, was applied to 22 subjects using a submaximal load of 40% estimated from an isometric MVC test. The neuromuscular activity of biceps brachii long head was compared by further partitioning each contraction into three phases, according to individual elbow joint range of motion. Although all protocols elicited a considerable level of activation of the biceps brachii muscle (at least 50% of maximum RMS, the contribution of this muscle for elbow flexion/extension varied among exercises. The submaximal elbow flexion (concentric elicited neuro muscular activity up to 95% of the maximum RMS value during the final phase of IDC and DBC and 80% for DPC at the beginning of the movement. All exercises showed significant less muscle activity for the elbow extension (eccentric. The Incline Dumbbell Curl and the classical Dumbbell Biceps Curl resulted in similar patterns of biceps brachii activation for the whole range of motion, whereas Dumbbell Preacher Curl elicited high muscle activation only for a short range of elbow joint angle

  2. Leaf curl diseases of two solanaceous species in Southwest Arabia are caused by a monopartite begomovirus evolutionarily most closely related to a species from the Nile Basin and unique suite of betasatellites

    KAUST Repository

    Idris, Ali

    2012-10-01

    The complete genome of 2780 bases was amplified using rolling circle amplification, and cloned, and sequenced for two distinct strains of the monopartite begomovirus Tomato leaf curl Sudan virus (ToLCSDV). The two strains shared 86-91% identity with the previously described ToLCSDV from the Nile Basin, and 90-91% identity with one another. One strain was cloned from symptomatic tomato plants from Tihamah (ToLCSDV-YE[YE:Tih:05]) while the other was cloned from symptomatic tobacco plants collected from Wadi Hadramaut (ToLCSDV-YE[YE:Had:89]). A distinct full-length betasatellite molecule (1352 bases) was cloned from the respective field-infected tomato and tobacco plants. Agro-inoculation of tomato and Nicotiana benthamiana plants with cloned partial tandem repeats of ToLCSDV-YE[YE:Tih11:05]) and the associated betasatellite, Tomato leaf curl Yemen betasatellite (ToLCYEB-[Tih:tom:137:05]), resulted in the reproduction of leaf curl disease symptoms in test plants like those observed in the field-infected plants. The betasatellite contributed to symptom severity in N. benthamiana test plants when it was co-inoculated with ToLCSDV-YE, compared to the milder symptoms that were observed in tobacco plants infected with the helper virus alone. © 2012 Elsevier B.V.

  3. Progress toward measles elimination—Philippines, 1998-2014.

    Science.gov (United States)

    Takashima, Yoshihiro; Schluter, W William; Mariano, Kayla Mae L; Diorditsa, Sergey; de Quiroz Castro, Maricel; Ou, Alan C; Ducusin, Maria Joyce U; Garcia, Luzviminda C; Elfa, Dulce C; Dabbagh, Alya; Rota, Paul; Goodson, James L

    2015-04-10

    In 2005, the Regional Committee for the World Health Organization (WHO) Western Pacific Region (WPR) established a goal to eliminate measles by 2012.The recommended elimination strategies in WPR include 1) ≥95% 2-dose coverage with measles-containing vaccine (MCV) through routine immunization services and supplementary immunization activities (SIAs); 2) high-quality case-based measles surveillance; 3) laboratory surveillance with timely and accurate testing of specimens to confirm or discard suspected cases and detect measles virus genotypes; and 4) measles outbreak preparedness, rapid response, and appropriate case management. In the WPR, the Philippines set a national goal in 1998 to eliminate measles by 2008. This report describes progress toward measles elimination in the Philippines during 1998-2014 and challenges remaining to achieve the goal. WHO-United Nations Children's Fund (UNICEF)-estimated coverage with the routine first dose of MCV (MCV1) increased from 80% in 1998 to 90% in 2013, and coverage with the routine second dose of MCV (MCV2) increased from 10% after nationwide introduction in 2010 to 53% in 2013. After nationwide SIAs in 1998 and 2004, historic lows in the numbers and incidence of reported measles cases occurred in 2006. Despite nationwide SIAs in 2007 and 2011, the number of reported cases and incidence generally increased during 2007-2012, and large measles outbreaks occurred during 2013-2014 that affected infants, young children, older children, and young adults and that were prolonged by delayed and geographically limited outbreak response immunization activities during 2013-2014. For the goal of measles elimination in WPR to be achieved, sustained investments are required in the Philippines to strengthen health systems, implement the recommended elimination strategies, and develop additional strategies to identify and reduce measles susceptibility in specific geographic areas and older age groups.

  4. Schistosomiasis and the Philippine campaign

    Institute of Scientific and Technical Information of China (English)

    Cristóbal S.Berry-Cabá

    2008-01-01

    Schistosomiasis is an infection with one of a series of related trematode parasites that are endemic to at least 76 tropical and subtropical countries.Schistosomiasis affects over 200 million people in tropical and subtropical regions of South America,Africa and Asia.This paper reported more than 1500 soldiers of US troops and 560 men of Australian Air Force were affected schistosomiasis japonicum in the Leyte of the Philippines during the liberation of the Philippines from Octorber 1944 to the early months of 1945.Many troops there were affected by contaminated water with snail hosts.At first,the medical corps knew little about the situations of the schis-tosomiasis japonica in the Leyte,no preventive measure was adopted.On December 23,1944 CPT morris Goldberg discovered schistosomes first from the hospitalized soldiers while conducting a liver biopsy.Some sol-diers with markedly signs and symptoms were admitted to hospital for treatment.Preventive measure was taken since.The US sent a lot of experts to Philippines for the research of schistosomiasis japonicum,including epi-demiology,prevention,diagnosis,treatment and so on.Nineteen chemicals were used to control snail hosts. In clinical,schistosomiasis has acute and chronic stages,the manifestations are plenty and various,the liver, intestine,veins,spleen and skin etc are common sites of impingement.An extensive educational program was carried out in the troops through different means,which yielded remarkable effect,the new incidence of schis-tosomiasis dropped.Though hospitalized soldiers were a few,but there were scare and some morale and psy-chological problems among them and the other affected victims,they worried about their prognoses or being in-fected.Some patients were evacuated to United States for treatment after diagnosis,some patients were treated in Leyte.Before World War II,schistosomiasis was treated generally with trivalent antimony compounds,Fua-din and tartar emetic.In this event,no case died

  5. Natural fertility in the Philippines.

    Science.gov (United States)

    Aquino, E G

    1982-01-01

    The hypothesis that modernization trends in the Philippines led to an increase in fecundity and natural fertility between 1953-1972 was tested, using data from the 1973 National Demographic Survey. More specifically, it was hypothesized that increases in education, income levels, urbanization, female labor force participation, and other factors exerted a positive influence on the population's health and nutritional status and increased the risk of pregnancy by diminishing the strength of sexual taboos and by decreasing the incidence of breastfeeding. These changes, in turn, had a positive impact on natural fertility. Natural fertility was defined as marital fertility in the absence of specific efforts to control fertility. The use of natural fertility instead of fecundity allowed for the influence of behavioral patterns, such as breastfeeding and sexual taboos, on fertility. Period analysis of age specific marital fertility rates for each 5 year period between 1953-72 and cohort analysis of age specific marital fertility rates for the birth cohorts, aged 55-59, 50-54, 45-49, 40-44, and 35-39 in 1973 were undertaken. The effect of fertility control was determined by using an index derived from an equation provided by Coale and Trussell. Findings of both the period and cohort analysis supported the hypothesis. Period analysis revealed that natural fertility increased between 1953-57 and 1969-72 by 10% and that the greatest increase occurred during the 1950s when Philippine society experienced major modernization changes. The increases in natural fertility were accompanied by corresponding increases in fertility regulation in each time period. These trends tended to cancel each other out and resulted in a relatively stable total marital fertility rate throughout the time period. Cohort analysis revealed that only the total marital fertility rate of the youngest cohort was influenced by fertility regulation. The level of natural fertility for all cohorts as a group

  6. Reliability of Arm Curl and Chair Stand tests for assessing muscular endurance in older people

    Directory of Open Access Journals (Sweden)

    Boneth M

    2012-05-01

    Full Text Available Objective: to assess the test-retest reliability and level of agreement between measures of the 30 second (30-s Arm Curl and 30-s Chair Stand test in a sample of older adults from Bucaramanga. Materials and methods: a study of evaluation of diagnostic technology was done. Both tests were administered by the same evaluator to 111 adults older than 59 year-old (70,4 ± 7,3, on two occasions, with an interval of time between measures of 4 to 8 days. In the analysis, test-retest reliability was determined using the Intraclass Correlation Coefficient ICC= 2,1 with their confidence interval 95% (CI 95% respective. The agreement level was established by the Bland and Altman method. Results: the test-retest reproducibility of the 30-s Arm Curl test was very good ICC= 0,88 and to the Chair Stand test was good ICC= 0,78. The agreement was very good for both tests of muscle endurance. The CI 95% were between -3,8 and 3,2 stands to 30-s Chair Stand test and between -3,1 and 2,8 curls to 30-s Arm Curl test. Conclusion: the 30-s Arm Curl and 30-s Chair Stand test have good reliability and agreement to assess muscle endurance in older adults functionally independent.

  7. The Economics of Counterinsurgency in the Philippines

    Science.gov (United States)

    2016-09-30

    Cambridge, MA 02138-5398 Under the direction of: Principal Investigator Ethan Kapstein, Arizona Centennial Professor oflnternational Affairs, Arizona...wellbeing; generate testable implications; test using data from Philippine insurgency. Develop implications for force configuration, rebel surrender

  8. Genetic counseling training in the Philippines.

    Science.gov (United States)

    Laurino, Mercy Ygona; Padilla, Carmencita David

    2013-12-01

    The recently established Master of Science in Genetic Counseling (MSGC) program serves a vital role in implementing and expanding genetic counseling services in the Philippines. Currently, only eight clinical geneticists practice in the Philippines, a country of approximately 94 million people, which yields a clinical-geneticist-to-population-density ratio of 1:11,750,000. The MSGC program was created to train health care providers to become crucial members of medical genetics teams being formed to meet increasing patient and healthcare provider demands. In 2011, the Board of Regents approved our proposed curriculum at the Department of Pediatrics College of Medicine, University of the Philippines Manila. As we relate how the Philippines began its efforts to implement the program and attempted to overcome the challenges the program faced, we hope we can provide an example to those interested in creating a similar MSGC program in other low-income and middle-income countries.

  9. Mount Pinatubo, Philippines: June 1991 Eruptions

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Mount Pinatubo is an andesitic island arc volcano, located on the southern Luzon Island, Philippines. Prior to 1991 it had been dormant for more than 635 years. On...

  10. Hydropower strategy for the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    McCandless, D.H. [Harza Consulting Engineers, Chicago, IL (United States)

    1995-11-01

    The government-owned National Power Corporation (NPC) of the Philippines is in the early stages of a complete privatisation programme. The electric power sector is expected eventually to be structured around a company that will own the major transmission facilities and will serve as a broker of power and energy between generating companies and the existing distribution companies, Rural Electric Co-operations and other power users. NPC`s non-transmission assets will probably be spun-off into various corporations including three generating companies, an engineering and services company and a company owning the Calivaya-Botocan-Kalayaan hydro complex. Plans for increased capacity include a real term increase in the hydropower contribution although it will decline as a percentage of the energy mix as coal and gas plants are developed. Details of the privatisation of specific hydropower projects in the Luzon, Visayas and Mindanao grids are described. (U.K.)

  11. Field Trial and Molecular Characterization of RNAi-Transgenic Tomato Plants That Exhibit Resistance to Tomato Yellow Leaf Curl Geminivirus.

    Science.gov (United States)

    Fuentes, Alejandro; Carlos, Natacha; Ruiz, Yoslaine; Callard, Danay; Sánchez, Yadira; Ochagavía, María Elena; Seguin, Jonathan; Malpica-López, Nachelli; Hohn, Thomas; Lecca, Maria Rita; Pérez, Rosabel; Doreste, Vivian; Rehrauer, Hubert; Farinelli, Laurent; Pujol, Merardo; Pooggin, Mikhail M

    2016-03-01

    RNA interference (RNAi) is a widely used approach to generate virus-resistant transgenic crops. However, issues of agricultural importance like the long-term durability of RNAi-mediated resistance under field conditions and the potential side effects provoked in the plant by the stable RNAi expression remain poorly investigated. Here, we performed field trials and molecular characterization studies of two homozygous transgenic tomato lines, with different selection markers, expressing an intron-hairpin RNA cognate to the Tomato yellow leaf curl virus (TYLCV) C1 gene. The tested F6 and F4 progenies of the respective kanamycin- and basta-resistant plants exhibited unchanged field resistance to TYLCV and stably expressed the transgene-derived short interfering RNA (siRNAs) to represent 6 to 8% of the total plant small RNAs. This value outnumbered the average percentage of viral siRNAs in the nontransformed plants exposed to TYLCV-infested whiteflies. As a result of the RNAi transgene expression, a common set of up- and downregulated genes was revealed in the transcriptome profile of the plants selected from either of the two transgenic events. A previously unidentified geminivirus causing no symptoms of viral disease was detected in some of the transgenic plants. The novel virus acquired V1 and V2 genes from TYLCV and C1, C2, C3, and C4 genes from a distantly related geminivirus and, thereby, it could evade the repressive sequence-specific action of transgene-derived siRNAs. Our findings shed light on the mechanisms of siRNA-directed antiviral silencing in transgenic plants and highlight the applicability limitations of this technology as it may alter the transcriptional pattern of nontarget genes.

  12. Social Protection and Disaster Risk Management in the Philippines

    OpenAIRE

    Bowen, Thomas

    2015-01-01

    This paper evaluates how the Philippines utilize social protection systems and programs to help households better manage disaster risk. Exposure and vulnerability to natural disasters and the effects of climate change are particularly high in the Philippines. At the same time, the Philippines has developed one of the most advanced social protection systems in the East Asia Pacific region. ...

  13. The University of San Carlos Herbarium, Cebu City, The Philippines

    NARCIS (Netherlands)

    Seidenschwarz, F.

    1990-01-01

    The University of San Carlos, Cebu City, (‘USC’) holds a botanical collection which is the fourth largest in size within the Philippines. The three leading herbaria of the Philippines are situated in or close to Manila. The 36 year old USC Herbarium is the only major collection in the Philippines ou

  14. Clinical innovations in Philippine thoracic surgery.

    Science.gov (United States)

    Danguilan, Jose Luis J

    2016-08-01

    Thoracic surgery in the Philippines followed the development of thoracic surgery in the United States and Europe. With better understanding of the physiology of the open chest and refinements in thoracic anesthetic and surgical approaches, Filipino surgeons began performing thoracoplasties, then lung resections for pulmonary tuberculosis and later for lung cancer in specialty hospitals dealing with pulmonary diseases-first at the Quezon Institute (QI) and presently at the Lung Center of the Philippines although some university and private hospitals made occasional forays into the chest. Esophageal surgery began its early attempts during the post-World War II era at the Philippine General Hospital (PGH), a university hospital affiliated with the University of the Philippines. With the introduction of minimally invasive thoracic surgical approaches, Filipino thoracic surgeons have managed to keep up with their Asian counterparts although the problems of financial reimbursement typical of a developing country remain. The need for creative innovative approaches of a focused multidisciplinary team will advance the boundaries of thoracic surgery in the Philippines.

  15. Leachable 226Ra in Philippine phosphogypsum and its implication in groundwater contamination in Isabel, Leyte, Philippines.

    Science.gov (United States)

    Cañete, Socrates Jose P; Palad, Lorna Jean H; Enriquez, Eliza B; Garcia, Teofilo Y; Yulo-Nazarea, Teresa

    2008-07-01

    Phosphogypsum (PG), the major waste material in phosphate fertilizer processing, has been known to contain enhanced levels of naturally-occurring radionuclides especially (226)Ra. The lack of radioactivity data regarding Philippine phosphogypsum and its environmental behavior in the Philippine setting has brought concern on possible contamination of groundwater beneath the phosphogypsum ponds in Isabel, Leyte, Philippines. The radioactivity of Philippine phosphogypsum was determined and the leaching of (226)Ra from phosphogypsum and through local soil was quantified. Level of (226)Ra in groundwater samples in Isabel, Leyte, Philippines was also quantified to address the primary concern. It was found that the (226)Ra activity in Philippine phosphogypsum is distributed in a wide range from 91.5 to 935 Bq/kg. As much as 5% of (226)Ra can be leached from Philippine PG with deionized water. In vitro soil leach experiments suggest that the soil in the phosphate fertilizer plant area would be able to deter the intrusion of (226)Ra into the water table. Compared to reported values of natural groundwater levels of (226)Ra, the concentration of this radionuclide in Isabel, Leyte groundwater suggest that there is no (226)Ra intrusion brought about by the presence of phosphogypsum ponds in the area.

  16. Analysis of resonance frequency and pull-in voltages of curled micro-bimorph cantilevers

    NARCIS (Netherlands)

    Abdulla, S.M.C.; Yagubizade, H.; Krijnen, G.J.M.

    2012-01-01

    A systematic study is presented on the modelling, fabrication and measurements of curled micro-bimorph cantilevers, which are composed of a dielectric beam with a metal electrode layer coated on top. The device, having stress-induced upward curvature in the electrical off-state, functions as a verti

  17. Effects of thermal treatments with a curling iron on hair fiber.

    Science.gov (United States)

    Ruetsch, S B; Kamath, Y K

    2004-01-01

    The effect of curling hair with a curling iron has been investigated. Possibilities of thermal damage with repeated curling according to, and in violation of, the manufacturer's specifications have been studied. The propensity of hair surface to damage depends on the moisture content of the hair, and these experiments have been conducted in both wet and dry conditions, with and without application of tension, and with short or prolonged times. Scanning electron microscopic (SEM) examination revealed that fibers treated under the dry condition (50% RH) show radial and axial cracking along with scale edge fusion. Similar thermal treatment on wet hair resulted in severe damage of the type described above, as well as bubbling and buckling of the cuticle due to the formation and escaping of steam from the fiber. Fibers subjected to repeated curling in the dry condition show slight increases in tensile mechanical properties, characteristic of a crosslinked fiber. Fibers treated with conditioners show an improvement in characteristic life, especially in the case of low-molecular-weight conditioners, such as CETAB, which can penetrate into the hair fiber (shown by TOF-SIMS analysis).

  18. Wind stress, curl and vertical velocity in the Bay of Bengal during southwest monsoon, 1984

    Digital Repository Service at National Institute of Oceanography (India)

    Babu, M.T.; Heblekar, A.K.; Murty, C.S.

    Wind distribution observed during southwest monsoon of 1984 has used to derive the mean wind stress for the season at every 1 degree square grid and curl over the Bay of Bengal. Two regions of maximum wind stress are present over the Bay of Bengal...

  19. Mainstreaming in the Philippines programme.

    Science.gov (United States)

    Smith, S

    1998-07-01

    In the Philippines, where the Oxfam team has worked on gender issues since 1990, the group recently conducted an in-house workshop to identify achievements and areas for improvement in gender mainstreaming. Gender activities began with a needs assessment of the fisheries in 1990 and have included gender-sensitivity training for Oxfam partner groups, support of women's issues, and gender research into such areas as disaster management. Successes include the appointment of women to key positions in the community, which was a direct result of advocacy work. Major challenges include 1) determining the best models to develop sectoral networking and advocacy on gender and 2) overcoming gender-resistance in some partners. The team concluded that it is still learning how to mainstream gender issues and is still searching for a framework to integrate gender into other approaches to planning, monitoring, and evaluation. In the future, Oxfam will 1) undertake small-scale and simple gender reviews with selected partners and then pilot gender-mainstreaming contracts with them, 2) build gender-learning teams within sectoral programs, 3) continue supporting networking and capability-building in feminist organizations, and 4) focus on gender at the next partners' meeting.

  20. Religious Tourism in Batangas, Philippines

    Directory of Open Access Journals (Sweden)

    Leonna Marrien U. Asi

    2015-11-01

    Full Text Available This study assessed religious tourism in Batangas, Philippines on the following aspects: historical significance, holiness and spirituality, and customs and traditions. The assessments made were also subjected to tests of differences when the respondents are grouped as to either residents or tourists and according to their age, sex, educational attainment and employment. Results show that both tourists and residents generally concurred on all religious tourism indicators presented to them for assessment, although there were also few indicators that had lower mean assessments than the others. While the differences on the assessments made by the different groups of respondents were not statistically significant, the slight differences are still noticeable. In particular, residents had somewhat more favourable assessment than the tourists, older respondents more than the younger, males more than the females, those with higher education more than those with lower education, and government employees more than those who are not in government service. These slight but still noticeable differences, together with religious tourism indicators having lower mean assessments than the others, can serve as bases for making proposals on how to further enrich the religious tourism in Batangas.

  1. Tectonics in the Northwestern West Philippine Basin

    Institute of Scientific and Technical Information of China (English)

    Ni Xianglong; Wu Shiguo; Shinjo Ryuichi

    2008-01-01

    The West Philippine basin (WPB) is a currently inactive marginal basin belonging to Philippine Sea plate, which has a complex formation history and various crust structures. Based on gravity, magnetic and seismic data, the tectonics in West Philippine basin is characterized by amagnma spreading stage and strike slip fractures. NNE trending Okinawa-Luzon fracture zone is a large fracture zone with apparent geomorphology and shows a right-handed movement. The results of joint gravity-magnetic-seismic inversion suggest that the Okinawa-Luzon fracture zone has intensive deformation and is a transform fault. Western existence of the NW trending fractures under Ryukyu Islands Arc is the main cause of the differences between south and north Okinawa Trough. The Urdaneta plateau is not a remained arc, but remnant of mantle plume although its lava chemistry is similar to oceanic island basalt (OIB).

  2. Utilization of electron accelerator in the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    Cabalfin, Estelita G. [Philippine Nuclear Research Institute, Quezon (Philippines)

    2003-02-01

    Radiosterilization of medical and surgical supplies, radiation treatment of consumer products such as food, pharmaceuticals and cosmetics, and the modification of polymers, crosslinking or curing, using gamma irradiation facilities in Philippine industries are overviewed. Philippine Nuclear Research Institute(PNRI) conducts bioburden determination, dose setting and validation of compatibility with radiation of product and packaging with the technical assistance of IAEA. The products with yields treated at the irradiation facility from 1996-2001 are shown in table. An electron accelerator of 10 MeV and 28 kW, established by Terumo Corporation, is used since 2000 for in-house radiation sterilization of syringes. Current regulations and regulatory authority in Philippines are also briefly introduced. Future processes such as radiation vulcanized natural rubber latex (RVNRL), use of carrageenan as hydrogel for dressing wounds and others and fermented bagasse as animal feed are now in progress. (S. Ohno)

  3. Security Force Assistance in the Philippines

    Science.gov (United States)

    2009-04-01

    34 ~"ř’ "CHINA,..-’ South ChintJ Sea -1:!" Luzon’ Strait ". , 1;:’~ 󈧄 200km 100 200nri Philippine Sea Source: The Central Intelligence Agency, The...tactics. The central Philippine government was generally uninterested because it was preoccupied with economic crises and corruption. is Insurgent Moro...making them extremely adept with speed and " banca " boats.3o The ASG worked with Ramzi Yoesef, mastermind of the 1993 World Trade Center bombings and

  4. Analyzing the influence of curl speed in fatiguing biceps brachii muscles using sEMG signals and multifractal detrended moving average algorithm.

    Science.gov (United States)

    Marri, Kiran; Swaminathan, Ramakrishnan

    2016-08-01

    In this work, an attempt has been made to analyze surface electromyography (sEMG) signals of fatiguing biceps brachii muscles at different curl speeds using multifractal detrended moving average (MFDMA) algorithm. For this purpose, signals are recorded from fifty eight healthy subjects while performing curl exercise at their comfortable speed until fatigue. The signals of first and last curls are considered as nonfatigue and fatigue conditions, respectively. Further, the number of curls performed by each subject and the endurance time is used for computing the normalized curl speed. The signals are grouped into fast, medium and slow using curl speeds. The curl segments are subjected to MFDMA to derive degree of multifractality (DOM), maximum singularity exponent (MXE) and exponent length multifractality index (EMX). The results show that multifractal features are able to differentiate sEMG signals in fatiguing conditions. The multifractality increased with faster curls as compared with slower curl speed by 12%. High statistical significance is observed using EMX and DOM values between curl speed and fatigue conditions. It appears that this method of analyzing sEMG signals with curl speed can be useful in understanding muscle dynamics in varied neuromuscular conditions and sports medicine.

  5. Philippine Local Government Delegation in China

    Institute of Scientific and Technical Information of China (English)

    Hu; Yang

    2013-01-01

    <正>A 20-member Philippine local government delegation headed by Victor A.Yap,Governor of Tarlac,visited China in early September and attended the 2013 China International Cities Cooperation Forum,a subforum of the China Jilin Northeast Asia Investment and Trade Expo held in Changchun,the provincial capital.CPAFFC President Li Xiaolin met

  6. Area Handbook Series. Philippines: A Country Study

    Science.gov (United States)

    1991-06-01

    Southeast Asian Studies, 1987. Villanueva , Honesto A. "The Independence Mission 1919: In- dependence Lies Ahead," Asian Studies [Manila], 9, No. 3...26, No. 5, May 1986, 501-17. Majul, Cesar Abid. "The Moros of the Philippines," Conflict, 8. Nos. 2-3, 1988, 169-84. Malajacan, Marcelino Q., Jr

  7. OUTLINE OF VOCATIONAL TRAINING IN THE PHILIPPINES.

    Science.gov (United States)

    Australian Dept. of Labour and National Service, Perth.

    THE PHILIPPINES HAVE A POPULATION OF 32 MILLION OF WHICH 60 PERCENT ARE ENGAGED IN AGRICULTURE. RECENT ECONOMIC DEVELOPMENT HAS INVOLVED INCREASING STABILITY TO PROVIDE A BASIS FOR GROWTH. THE EDUCATION SYSTEM IS BASED ON A 6-YEAR ELEMENTARY AND A 4-YEAR SECONDARY SCHOOL COURSE. SECONDARY EDUCATION IS PROVIDED AT EITHER A GENERAL SCHOOL OR A…

  8. Studies in Philippine Languages and Cultures.

    Science.gov (United States)

    Brainard, Sherri, Ed.

    2001-01-01

    This issue of Studies in Philippine Languages and Cultures contains the following articles: "Functions of Locatives in Northern Subanen Expository and Hortatory Discourse" (Josephine Sanicas-Daguman); "Functions of Demonstratives in Sama Bangingi' Expository Discourse" (John Blakely); "A Brief Look at Sinama Basic Verbs…

  9. Philippines: Asia Pacific energy series: Country report

    Energy Technology Data Exchange (ETDEWEB)

    Hoffman, S.

    1988-11-01

    The purpose of this report is to present an overview of Philippines energy planning and policy and to analyze its energy resources and economic developments. Geography, population, geothermal reserves, coal, oil industry, electricity, and renewable energy are the major topics included in the report. A chapter is focused on national economy and energy policy. 40 tabs., 2 figs.

  10. Micro hydro demo in the Philippines

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The Philippine department of Energy (DOE) has signed a memorandum of agreement with the Japanese government for a P33.SM (US$0.67M) demonstration project. The 65kW micro hydro power system will benefit about 200 households in Leyte.

  11. Taxonomy and conservation of Philippine Cycads

    NARCIS (Netherlands)

    Madulid, D.A.; Agoo, E.M.G.

    2009-01-01

    Six species of cycads are recorded in the Philippines, three of which are endemic. The different species of cycads can be recognized by the characters of the microsporophylls, megasporophylls, and seeds. The current conservation status of the different species of Cycas is assessed and categorized us

  12. Game Theory, People Power and Philippine Politics.

    Science.gov (United States)

    Lee-Chua, Queena N.

    2000-01-01

    Delineates two illustrative game-theoretic applications to Philippine politics: (1) People Power Revolution in the mid-1980s and (2) conflict over Spratly Islands in the mid-1990s. Uses zero-sum games to model these two events, and elementary matrix theory to determine pure strategies and locate equilibrium points. Includes recommendations for…

  13. Power barges ease Philippines power shortage

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1996-02-01

    The Navotas barge project has been a cornerstone in helping the Philippines through the power crisis that almost brought the economy to a standstill. Van der Horst`s stake in the project recently reached more than 240 MW with the delivery of the latest of its four power barges. (author)

  14. Guiding Principle 27 and Philippine typhoon response

    Directory of Open Access Journals (Sweden)

    Sara McHattie

    2008-12-01

    Full Text Available Oxfam GB’s response to the devastation and displacementcaused by Typhoon Durian included advocacy withPhilippine state, NGO, community and private sectoractors to raise awareness of Principle 27 – obligingproviders of humanitarian assistance to “give due regardto the protection needs and human rights” of IDPs.

  15. Researching English in the Philippines: Bibliographical Resources

    Science.gov (United States)

    Bautista, Ma. Lourdes S.

    2004-01-01

    The academic literature on issues related to the Philippine English language and literature is substantial. This bibliography surveys relevant work on such related fields as the sociology of language and language planning, Bilingualism, bilingual education, and languages in education, language attitudes, code-switching and code-mixing, Philippine…

  16. Philippines : PPP Options in Irrigation Sector

    OpenAIRE

    World Bank

    2013-01-01

    This study proposes an analytical framework to determine the extent to which Public-Private Partnership (PPP) is a viable and effective business option for developing and modernizing the irrigation sector in the Philippines. It focuses on: (a) assessing and providing recommendations to address the policy, regulatory and institutional constraints that limit the recourse to PPP as a tool to ...

  17. Student thinking about the divergence and curl in mathematics and physics contexts

    CERN Document Server

    Baily, Charles; Pattie, Andrew; van Kampen, Paul; De Cock, Mieke

    2015-01-01

    Undergraduate physics students are known to have difficulties with understanding mathematical tools, and with applying their knowledge of mathematics to physical contexts. Using survey statements based on student interviews and written responses to open-ended questions, we investigated the prevalence of correct and incorrect conceptions regarding the divergence and curl of vector fields, among both mathematics and physics students. We compare and contrast pre-instruction responses from intermediate-level E&M students at KU Leuven and the University of St Andrews with post-instruction responses from St Andrews students enrolled in a vector calculus course. In comparing pre- and post-instruction responses from E&M students we see that, although their understanding of the divergence and curl improved, relatively few of them were able to provide completely correct survey responses at mid-semester.

  18. A simple and straightforward expression for curling probe electron density diagnosis in reactive plasmas

    Science.gov (United States)

    Arshadi, Ali; Brinkmann, Ralf Peter; Hotta, Masaya; Nakamura, Keiji

    2017-04-01

    Active plasma resonance spectroscopy (APRS) refers to the family of plasma diagnostic methods which utilize the ability of plasmas to resonate at frequencies close to the plasma frequency. APRS operates by exciting the plasma with a weak RF signal by means of a small electric probe. The response of the plasma is recorded by a network analyzer (NA). A mathematical model is applied to derive characteristics like the electron density and the electron temperature. The curling probe is a promising realization of APRS. The curling probe is well-qualified for the local measurement of the electron density in reactive plasmas. This spiral probe resonates in plasma at a larger density dependent frequency than the plasma frequency. This manuscript represents a simple and straightforward expression relating this resonance frequency to the electron density of the plasma. A good agreement is observed between the proposed expression and the results obtained from previous studies and numerical simulations.

  19. Efficient Assembly of H(div) and H(curl) Conforming Finite Elements

    CERN Document Server

    Rognes, Marie; Logg, Anders; 10.1137/08073901X

    2012-01-01

    In this paper, we discuss how to efficiently evaluate and assemble general finite element variational forms on H(div) and H(curl). The proposed strategy relies on a decomposition of the element tensor into a precomputable reference tensor and a mesh-dependent geometry tensor. Two key points must then be considered: the appropriate mapping of basis functions from a reference element, and the orientation of geometrical entities. To address these issues, we extend here a previously presented representation theorem for affinely mapped elements to Piola-mapped elements. We also discuss a simple numbering strategy that removes the need to contend with directions of facet normals and tangents. The result is an automated, efficient, and easy-to-use implementation that allows a user to specify finite element variational forms on H(div) and H(curl) in close to mathematical notation.

  20. Effect of Performance Speed on Trunk Movement Control During the Curl-Up Exercise

    Directory of Open Access Journals (Sweden)

    Barbado David

    2015-06-01

    Full Text Available Trunk exercise speed has significant effects on neuro-mechanical demands; however, the influence of a variety of exercise speeds on motor control of the trunk displacement remains unknown. The aim of this study was to assess the effect of performance speed on trunk motion control during the curl-up exercise by analyzing the kinematic variance about the sagittal trajectory. Seventeen subjects volunteered to perform curl-ups at different cadences controlled by a metronome. Standard deviation (SD and range (RG of shoulder girdle medial-lateral displacement (SGML and detrended fluctuation analysis (DFA of SGML were calculated to examine linear variability and long range autocorrelation of medial-lateral upper trunk displacements, respectively. In addition, SD, RG and DFA of centre of pressure medial-lateral displacement (COPML were performed to analyze the behavior of the motor system while controlling trunk displacement. Although SD and RG of COPML increased as speed increased, the curl-up cadence did not have significant effects on SD and RG of SGML. These results suggest that although high speed curl-ups challenged participants’ ability to carry out medial-lateral adjustments, an increase of performance speed did not modify the linear variability about the sagittal trajectory. Regarding DFA, the scaling exponent α of SGML and COPML was higher for the fastest movements, mainly in long term fluctuations. Therefore, to maintain the target trajectory, participants used different strategies depending on performance speed. This is to say, there were less trajectory changes when participants performed the fastest exercises.

  1. Qualitative investigation into students' use of divergence and curl in electromagnetism

    Science.gov (United States)

    Bollen, Laurens; van Kampen, Paul; Baily, Charles; De Cock, Mieke

    2016-12-01

    Many students struggle with the use of mathematics in physics courses. Although typically well trained in rote mathematical calculation, they often lack the ability to apply their acquired skills to physical contexts. Such student difficulties are particularly apparent in undergraduate electrodynamics, which relies heavily on the use of vector calculus. To gain insight into student reasoning when solving problems involving divergence and curl, we conducted eight semistructured individual student interviews. During these interviews, students discussed the divergence and curl of electromagnetic fields using graphical representations, mathematical calculations, and the differential form of Maxwell's equations. We observed that while many students attempt to clarify the problem by making a sketch of the electromagnetic field, they struggle to interpret graphical representations of vector fields in terms of divergence and curl. In addition, some students confuse the characteristics of field line diagrams and field vector plots. By interpreting our results within the conceptual blending framework, we show how a lack of conceptual understanding of the vector operators and difficulties with graphical representations can account for an improper understanding of Maxwell's equations in differential form. Consequently, specific learning materials based on a multiple representation approach are required to clarify Maxwell's equations.

  2. Chopping of near- and mid-infrared radiation using a curled electrostatic MEMS actuator

    Science.gov (United States)

    Dausch, David E.; Goodwin, Scott H.; Exarhos, Gregory J.

    2003-09-01

    An electrostatic MEMS actuator known as the "Artificial Eyelid" can be used as a micromechanical chopper for IR detectors. The actuator structure consists of a curled polymer/metal film stack which is microfabricated and released from an IR transparent substrate. The film stack is uncurled by applying an electric field between the curled film and the transparent fixed electrode on the substrate. These flexible film actuators can act as IR choppers, providing transmission of radiation to the sensor elements when open (curled) and reflection when closed (uncurled). Arrays of actuators were fabricated on ITO-coated glass substrates and ranged in size from 4 x 4 mm to 7.5 x 15 mm with individual elements ranging from 250 to 500 μm on a side. Actuation for devices with average radius of curvature of 120 μ was consistently achieved at 150-170 V operation with 98-100% of the elements functioning and long lifetimes. IR chopper characteristics were measured using a blackbody source and pyroelectric detector by applying sine and square wave voltage to the actuators at a frequency of 30 Hz. The capability of the artificial eyelid for chopping near- and mid-IR radiation, including future fabrication of devices using NiCo2O4 or NiRh2O4 films for IR transparent electrodes, will be discussed.

  3. Southern Philippines Public Perception Survey Exploration and Analysis

    Science.gov (United States)

    2013-07-31

    23 Table 3. Factor Analysis Results for Cotabato. The dark shaded region of the tables indicates common factors with common loadings, the lighter... tourism , security, etc. It is home to Western Mindanao Command and some of the Philippine Air Forces. It also enjoys reliable power most of the time...Philippines as part of a widespread Islamic state which would span Indonesia, Malaysia , southern Thailand, Singapore, Brunei, and the southern Philippines

  4. US influence on military professionalism in the Philippines

    OpenAIRE

    2003-01-01

    Approved for public release; distribution is unlimited The "special relationship" between the US and the Philippines date back to the beginning of the 20th Century. During this period, the US played a significant role in the development of the Armed Forces of the Philippines (AFP). This thesis assesses the scope of US influence in shaping military professionalism in the Philippines. Military professionalism is essential in keeping positive civil-military relations and sustaining democracy....

  5. Labor mobility, trade and structural change: the Philippine experience.

    Science.gov (United States)

    Abella, M I

    1993-01-01

    "This article addresses three questions: (1) Is the high rate of emigration of labor from the Philippines related to the country's trade policy? (2) Why have migration and accompanying remittances not made much of an impact on the growth and structure of the Philippine economy? (3) Would economic growth and structural change eventually curtail labor emigration? The Philippines' history of labor export and its economic development are contrasted with those of Asian NIEs [newly industrialized economies] which have adopted liberal trade regimes."

  6. Post-disaster resettlement in the Philippines: a risky strategy

    Directory of Open Access Journals (Sweden)

    Alice R Thomas

    2015-05-01

    Full Text Available Experience in the Philippines following Typhoon Haiyan suggests that resettlement as a strategy for mitigating disaster-induced displacement can create significant protection risks.

  7. A Case for Cohabitative Security: The Philippine and Malaysian Experience

    National Research Council Canada - National Science Library

    Michael Intal Magcamit

    2014-01-01

    .... Using the Philippines and Malaysia as case studies, the article determines the extent to which public officials and policymakers have redefined and reenvisioned national security by incorporating non...

  8. International nurse migration: lessons from the Philippines.

    Science.gov (United States)

    Brush, Barbara L; Sochalski, Julie

    2007-02-01

    Developed countries facing nursing shortages have increasingly turned to aggressive foreign nurse recruitment, primarily from developing nations, to offset their lagging domestic nurse supplies and meet growing health care demands. Few donor nations are prepared to manage the loss of their nurse workforce to migration. The sole country with an explicit nurse export policy and the world's leading donor of nurse labor - the Philippines - is itself facing serious provider maldistribution and countrywide health disparities. Examining the historical roots of Philippines nurse migration provides lessons from which other nurse exporting countries may learn. The authors discuss factors that have predicated nurse migration and policies that have eased the way. Furthermore, the authors analyze how various stakeholders influence migratory patterns, the implications of migration for nurses and the public in their care, and the challenges that future social policy and political systems face in addressing global health issues engendered by unfettered recruitment of nurses and other health workers.

  9. Tourism and Crime: Evidence from the Philippines

    OpenAIRE

    Rosalina Palanca-Tan; Garces, Len Patrick Dominic M.; Angelica Nicole C. Purisima; Zaratan, Angelo Christian L.

    2015-01-01

    Using panel data gathered from 16 regions of the Philippines for the period 2009–11, this paper investigates the relationship between tourism and crime. The findings of the study show that the relation between tourism and crime may largely depend on the characteristics of visitors and the types of crime. For all types of crime and their aggregate, no significant correlation between the crime rate (defined as the number of crime cases divided by population) and total tourist arrivals is found....

  10. The Philippines’ Contribution to Hemispheric Defense

    Science.gov (United States)

    1949-05-10

    corn, tobacco and maguey. The principal fruit is the pine- apple but there are also mangoes , papaya, lanzones, pilinut, chico, mandarins and oranges...privately owned property. The United States shall pay just compensation for injury to persons or damage to property that may result from action taken in...property, both real and personal, or personal injury or death of inhabitants of the Philippines, when such damage, loss destruction or injury is caused

  11. The Philippines in a Changing Southeast Asia

    Science.gov (United States)

    1989-03-01

    FORCES OF THE PHILIPPINES AND THE AQUINO REGIME By Benjamin N. Muego, Bowling Green State University It has been more than three years since Corazon ...metPiin fie,94. Puluhan, Guerrero de Jesus, Kilusan Laban sa Komunista, Nakasaka, Surit-Surit, and Sagrado Corazon del Senor. For 50. Almonte, op...34reformists" abortive effort to bring arms from China via the ship Karagaan, such as Corazon Aquino in a coalition calling for Marcos to step which was

  12. Clinical innovations in Philippine thoracic surgery

    OpenAIRE

    Jose Luis. J. Danguilan

    2016-01-01

    Thoracic surgery in the Philippines followed the development of thoracic surgery in the United States and Europe. With better understanding of the physiology of the open chest and refinements in thoracic anesthetic and surgical approaches, Filipino surgeons began performing thoracoplasties, then lung resections for pulmonary tuberculosis and later for lung cancer in specialty hospitals dealing with pulmonary diseases—first at the Quezon Institute (QI) and presently at the Lung Center of the P...

  13. Origins of the 1986 Philippine Constitution

    Science.gov (United States)

    1993-04-01

    instituted the encomienda system, similar to the feudal system of old, primarily to reward Spaniards who had rendered service in the conquest of the...Philippines. The encomiendas were large parcels of land including its inhabitants and resources. The encomendero, or owner of the encomienda , ruled...over the inhabitants and kept order by the execution of laws created mostly by decree. The encomienda was abolished after only a few generations of

  14. Excerpt from Dead Stars: American and Philippine Literary Perspectives on the American Colonization of the Philippines

    Directory of Open Access Journals (Sweden)

    Jennifer M. McMahon

    2011-12-01

    Full Text Available Dead Stars: American and Philippine Literary Perspectives on the American Colonization of the Philippines examines the American colonization of the Philippines from three distinct but related literary perspectives. The first is the reaction of anti-imperialist American writers Mark Twain, W. E. B. Du Bois, and William James to America’s first foray into the role of colonizer and how their varied essays, letters, and speeches provide an incisive delineation of fundamental conflicts in American identity at the turn of the twentieth century. The book then analyzes how these same conflicts surface in the colonial regime’s use of American literature as a tool to inculcate American values in the colonial educational system. Finally, Dead Stars considers the way three early and important Filipino writers—Paz Marquez Benitez, Maximo Kalaw, and Juan C. Laya—interpret and represent these same tensions in their fiction.

  15. Development of radioisotope production in the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    Cabalfin, E.G. [Philippine Nuclear Research Institute, Quezon (Philippines)

    1998-10-01

    The Philippine Nuclear Research Institute (PNRI) started its activities on radioisotope production more than three decades ago, when the Philippine Research Reactor (PRR-1) started operating at its full rated power of 1 MW. Since then, several radionuclides in different chemical forms, were routinely produced and supplied for use in nuclear medicine, industry, agriculture, research and training, until the conversion of the PRR-1 to a 3 MW TRIGA type reactor. After the criticality test of the upgraded reactor, a leak was discovered in the pool liner. With the repair of the reactor still ongoing, routine radioisotope production activities have been reduced to dispensing of imported bulk {sup 131}I. In the Philippines, radioisotopes are widely used in nuclear medicine, with {sup 131}I and {sup 99m}Tc as the major radionuclides of interest. Thus the present radioisotope production program of PNRI is directed to meet this demand. With the technical assistance of the International Atomic Energy Agency (IAEA), PNRI is setting up a new {sup 131}I production facility. The in-cell equipment have been installed and tested using both inactive and active target, obtained from BATAN, Indonesia. In order to meet the need of producing {sup 99}Mo-{sup 99m}Tc generators, based on low specific activity reactor-produced {sup 99}Mo, research and development work on the preparation of {sup 99m}Tc gel generators is ongoing. (author)

  16. Photovoltaic battery charging experience in the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    Navarro, S.T. Jr.

    1997-12-01

    With the turn of the century, people in remote areas still live without electricity. Conventional electrification will hardly reach the remaining 50% of the population of the Philippines in remote areas. With photovoltaic technology, the delivery of electricity to remote areas can be sustainable. Malalison island was chosen as a project site for electrification using photovoltaic technology. With the fragile balance of ecology and seasonal income in this island, the PV electrification proved to be a better option than conventional fossil based electrification. The Solar Battery Charging Station (SBCS) was used to suit the economic and geographical condition of the island. Results showed that the system can charge as many as three batteries in a day for an average fee of $0.54 per battery. Charging is measured by an ampere-hour counter to determine the exact amount of charge the battery received. The system was highly accepted by the local residents and the demand easily outgrew the system within four months. A technical, economic and social evaluation was done. A recovery period of seven years and five months is expected when competed with the conventional battery charging in the mainland. The technical, economic, institutional and social risks faced by the project were analyzed. Statistics showed that there is a potential of 920,000 households that can benefit from PV electrification in the Philippines. The data and experiences gained in this study are valuable in designing SBCS for remote unelectrified communities in the Philippines and other developing countries.

  17. Genetics and Genomics of Cotton Leaf Curl Disease, Its Viral Causal Agents and Whitefly Vector: A Way Forward to Sustain Cotton Fiber Security

    Directory of Open Access Journals (Sweden)

    Mehboob-ur- Rahman

    2017-07-01

    Full Text Available Cotton leaf curl disease (CLCuD after its first epidemic in 1912 in Nigeria, has spread to different cotton growing countries including United States, Pakistan, India, and China. The disease is of viral origin—transmitted by the whitefly Bemisia tabaci, which is difficult to control because of the prevalence of multiple virulent viral strains or related species. The problem is further complicated as the CLCuD causing virus complex has a higher recombination rate. The availability of alternate host crops like tomato, okra, etc., and practicing mixed type farming system have further exaggerated the situation by adding synergy to the evolution of new viral strains and vectors. Efforts to control this disease using host plant resistance remained successful using two gene based-resistance that was broken by the evolution of new resistance breaking strain called Burewala virus. Development of transgenic cotton using both pathogen and non-pathogenic derived approaches are in progress. In future, screening for new forms of host resistance, use of DNA markers for the rapid incorporation of resistance into adapted cultivars overlaid with transgenics and using genome editing by CRISPR/Cas system would be instrumental in adding multiple layers of defense to control the disease—thus cotton fiber production will be sustained.

  18. Genetics and Genomics of Cotton Leaf Curl Disease, Its Viral Causal Agents and Whitefly Vector: A Way Forward to Sustain Cotton Fiber Security.

    Science.gov (United States)

    Rahman, Mehboob-Ur-; Khan, Ali Q; Rahmat, Zainab; Iqbal, Muhammad A; Zafar, Yusuf

    2017-01-01

    Cotton leaf curl disease (CLCuD) after its first epidemic in 1912 in Nigeria, has spread to different cotton growing countries including United States, Pakistan, India, and China. The disease is of viral origin-transmitted by the whitefly Bemisia tabaci, which is difficult to control because of the prevalence of multiple virulent viral strains or related species. The problem is further complicated as the CLCuD causing virus complex has a higher recombination rate. The availability of alternate host crops like tomato, okra, etc., and practicing mixed type farming system have further exaggerated the situation by adding synergy to the evolution of new viral strains and vectors. Efforts to control this disease using host plant resistance remained successful using two gene based-resistance that was broken by the evolution of new resistance breaking strain called Burewala virus. Development of transgenic cotton using both pathogen and non-pathogenic derived approaches are in progress. In future, screening for new forms of host resistance, use of DNA markers for the rapid incorporation of resistance into adapted cultivars overlaid with transgenics and using genome editing by CRISPR/Cas system would be instrumental in adding multiple layers of defense to control the disease-thus cotton fiber production will be sustained.

  19. Effect of ambient storage on the quality characteristics of aerobically packaged fish curls incorporated with different flours.

    Science.gov (United States)

    Raja, Waseem Hussain; Kumar, Sunil; Bhat, Zuhaib Fayaz; Kumar, Pavan

    2014-01-01

    The present study was conducted to evaluate the effect of ambient storage on the quality attributes of aerobically packaged fish curls incorporated with optimum levels of different flours. The curls were developed by extrusion technology using fish meat (Catla catla). The fish curls containing optimum levels of different flours viz. 20 percent corn flour, 10 percent black gram flour and 10 percent peanut flour were compared with the control snacks containing 30 percent rice flour and assessed for storage quality and shelf life at ambient temperature. The curls were aerobically packaged in LDPE (low density polyethylene) pouches and evaluated for various physicochemical, microbiological and sensory parameters. Mean values of pH of all the curls showed significantly (p flour incorporated samples, 6.36 ± 0.01 on day 0 and 6.14 ± 0.01 on day 28 for black gram flour incorporated samples, 6.57 ± 0.007 on day 0 and 6.34 ± 0.01 on day 28 for peanut flour incorporated samples). TBARS (mg malonaldehyde/kg), total plate count (log cfu/g) and yeast and mould count (log cfu/g) for the control as well as treatment samples showed significantly (p flours were acceptable up to 21 days of ambient storage within the LDPE pouches.

  20. Plurality in Unity: Challenges toward Religious Education in the Philippines

    Science.gov (United States)

    Baring, Rito V.

    2011-01-01

    This article reviews the challenges provided by a plural condition toward doing religious education in the Philippines. The problem of Philippine religious education hinges on the fact that the growing plural condition in the educational system remains until now "un-discussed"; or integrated in many schools. Not much is heard about proposing a…

  1. Revision of the genus Cleistanthus (Euphorbiaceae) in the Philippines

    NARCIS (Netherlands)

    Dressler, Stefan

    1999-01-01

    The Philippine species of the euphorbiaceous genus Cleistanthus are revised. Sixteen species are recognised for this archipelago of which two are recorded from there for the first time. The oldest available combination from the Philippines [C. orgyalis (Blanco) Merr.] remains obscure and three colle

  2. News Reporting in the Philippines: English in Print Media

    Science.gov (United States)

    Dayag, Danilo T.

    2010-01-01

    This study aims to determine how a hostage drama in Iraq involving an overseas Filipino worker was framed in English-language newspapers in the Philippines. Data came from the July 9-25, 2004 issues of five leading English-language broadsheets in the Philippines. The study found that the event was given maximum salience and prominence by the…

  3. Conservation agriculture for food security in the Philippines

    OpenAIRE

    University of the Philippines - Los Baños

    2010-01-01

    This brochure describes the project to promote Conservation Agriculture as a technologically-feasible, economically-viable, environmentally-sustainable and gender-responsive production system that will contribute to food security of small farm communities in the Philippines. LTRA-12 (Conservation agriculture for food security in Cambodia and the Philippines)

  4. Bringing Reliable Electricity to Rural Areas of the Philippines

    OpenAIRE

    Cariaga, Art; Jed A. Sevilla; Fernando-Pacua, Marianna; Beloe, William Trant

    2009-01-01

    Electric Cooperatives (ECs) in the Philippines are generally undercapitalized, and their operational performance is poor. As a result, the supply of electricity in rural areas is unstable, with frequent outages and fluctuating voltages. International Finance Corporation (IFC) Philippines advisory services found that a principal reason was the ECs' lack of sound capital expenditure (capex) ...

  5. Higher education accreditation in the Philippines: A literature review

    Directory of Open Access Journals (Sweden)

    Ching, Gregory S.

    2013-01-01

    Full Text Available This paper discusses the historical perspective, rationales, implications, different types and processes that undergoes in the accreditation of private higher educational institutions in the Philippines. As a result, higher education accreditation in the Philippines is centered on four key result areas, namely: quality of teaching and research, support for students, relations with the community, and management of resources.

  6. 78 FR 57620 - Trade Mission to Philippines and Malaysia

    Science.gov (United States)

    2013-09-19

    ... International Trade Administration Trade Mission to Philippines and Malaysia AGENCY: International Trade... FR 22237, April 15, 2013, regarding the education industry trade mission to Manila, Philippines and Kuala Lumpur, Malaysia scheduled for October 23-October 30, 2013, to revise the mission description from...

  7. A Case Study on Special Education in the Philippines.

    Science.gov (United States)

    Pascual, Dolores M.; Gregorio, Julieta A.

    This case study, undertaken as part of a Unesco survey of its member states, documents facilities and services for the education of disabled persons in the Philippines. The case study reports that Philippine children and youth with special needs are provided with access to educational opportunities that develop their potential and enable them to…

  8. A Case Study on Special Education in the Philippines.

    Science.gov (United States)

    Pascual, Dolores M.; Gregorio, Julieta A.

    This case study, undertaken as part of a Unesco survey of its member states, documents facilities and services for the education of disabled persons in the Philippines. The case study reports that Philippine children and youth with special needs are provided with access to educational opportunities that develop their potential and enable them to…

  9. Regulatory and Skills Requirements for Higher Education in the Philippines

    Science.gov (United States)

    Adeyemo, Kolawole Samuel

    2015-01-01

    The provision of public resources to manage the expansion of the higher education system in the Philippines has been inadequate, and this has given rise to many private providers entering the HE domain. The proper regulation of higher education in the country is important if the Philippines is to respond to the challenge of producing the skills it…

  10. Fictionalized History in the Philippines: Five Narratives of Collective Amnesia

    Science.gov (United States)

    Ortiz, Will P.

    2008-01-01

    The paper analyzes five historical fictions for children in the Batang Historyador (Young Historian) series which detail five periods in Philippine history. The books discuss the issues of child labor in precolonial Philippines, child labor and the right to education regardless of gender during the Spanish colonial period, child labor during the…

  11. 7 CFR 319.56-33 - Mangoes from the Philippines.

    Science.gov (United States)

    2010-01-01

    ... Administrator has determined meets the criteria set forth in § 319.56-5 with regard to the mango seed weevil... 7 Agriculture 5 2010-01-01 2010-01-01 false Mangoes from the Philippines. 319.56-33 Section 319.56... Mangoes from the Philippines. Mangoes (fruit) (Mangifera indica) may be imported into the United...

  12. Attentional Demands in the Execution Phase of Curling in Novices and Experts

    Directory of Open Access Journals (Sweden)

    Véronique Shank

    2013-03-01

    Full Text Available The purpose of this study was to determine the attentional demands of a curling delivery using a dual-task procedure. Skilled and novice curlers were asked to perform take outs and draws while attentional demands were measured using reaction time (RT in the different phases of the throw. Results showed attentional demands for the draw and take out were highest at the beginning of the delivery. Compared to the draw, a significant rise of RT was shown at the end of the take out shot. Shot success was significantly higher for the take out condition in expert compared to novice curlers.

  13. Curling up two spatial dimensions with SU(1,1)/U(1)

    Energy Technology Data Exchange (ETDEWEB)

    Gell-Mann, M.; Zwiebach, B.

    1984-11-01

    It is seen that a nonlinear sigma model based on the noncompact coset space SU(1,1)/U(1) can curl up two spatial dimensions into a topologically noncompact surface of finite area with a compact U(1) isometry group. This mechanism can be used for several higher-dimensional supergravity theories. In particular, chiral N = 2, D = 10 supergravity would reduce to an N = 1, D = 8 theory in which the masslessness of fermions does not depend only on supersymmetry. Further reduction to four dimensions is possible.

  14. Parallel H1-based auxiliary space AMG solver for H(curl) problems

    Energy Technology Data Exchange (ETDEWEB)

    Kolev, T V; Vassilevski, P S

    2006-06-30

    This report describes a parallel implementation of the auxiliary space methods for definite Maxwell problems proposed in [4]. The solver, named AMS, extends our previous study [7]. AMS uses ParCSR sparse matrix storage and the parallel AMG (algebraic multigrid) solver BoomerAMG [1] from the hypre library. It is designed for general unstructured finite element discretizations of (semi)definite H(curl) problems discretized by Nedelec elements. We document the usage of AMS and illustrate its parallel scalability and overall performance.

  15. Fatal poisoning by Rumex crispus (curled dock): pathological findings and application of scanning electron microscopy.

    Science.gov (United States)

    Reig, R; Sanz, P; Blanche, C; Fontarnau, R; Dominguez, A; Corbella, J

    1990-10-01

    A case of fatal poisoning due to ingestion of the plant Rumex crispus (curled dock) is described. The patient, a 53-year-old male, presented with gastrointestinal symptoms, severe hypocalcemia, metabolic acidosis and acute hepatic insufficiency. Despite therapeutic measures, the patient died 72 h after ingestion of the plant material. Noteworthy among the pathological findings were centrolobular hepatic necrosis and birefringent crystals in the liver and kidneys that were identified by histochemical techniques and scanning electron microscopy. These observations are compared with other reports in the medical literature, with an emphasis on the risk involved in the use of these plants for culinary or medicinal purposes.

  16. 中国男子冰壶队与欧美强队扫冰技术能力对比--以2014男子冰壶世界锦标赛为例%Technical Ability Comparative of Sweeping of Chinese Curling Male Team and Euro-American Curling Strong Teams--An Example of 2014 World Curling Male Championship

    Institute of Scientific and Technical Information of China (English)

    马永俊; 牛晓暹; 臧荣海; 许水生

    2014-01-01

    冰壶运动中扫冰技术是对比赛过程产生影响的重要因素。利用文献资料、现场观察等研究方法,对2014男子冰壶世界锦标赛中国队与欧美强队扫冰技术能力进行对比分析,从中找出中国男子冰壶队与欧美强队在扫冰技术能力上的差别,提高中国男子冰壶队竞赛成绩。结果表明:扫冰技术在冰壶比赛中极为重要,对比赛的过程产生重要的影响;中国队与欧美强队相比扫冰技术能力相对较弱;在队投壶技术的稳定性方面中国队比欧美强要好。建议中国队在扫冰技术方面进行针对性训练,加强对冰壶运动轨迹产生影响因素的综合理解认识;增强扫冰技术团队配合能力;提高扫冰技术专项力量训练。%Sweeping is an important factor to influence a process of a game in curling,with the use the methods of literature,field observation and so on. By comparing analyzing on the sweeping technical ability of Chinese curling male team and Euro-American Curling strong teams,find out the difference of technical ability of Chinese curling male team and Euro-American Curling strong teams,to improve the performance of Chinese curling male team. The results show:sweeping technology is very important in curling competition,and have an important impact on the process of games;The sweeping technical ability of Chinese Team is relatively weak to Europe-American strong teams;The stability of throwing curling technology of Chinese Team is better than Europe-American strong teams. Suggest Chinese to have targeted training in sweeping technology, strengthen the comprehensive understanding of curling motion track;Enhance the cooperated ability of sweeping technology;Improve the special strength training of sweeping technology.

  17. Photovoltaics provide electricity to rural communities in the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-31

    Almost a million rural households in the Visayas region of the Philippines have no electrical power. Solar Resources Inc of Wilmington, Massachusetts has entered into a joint venture with three Philippine companies to install and monitor 18 residential photovoltaic demonstration systems to provide electricity to rural families for lighting and radio or television. The joint venture plans to install 2,000 more systems over the next 16 months. Members of rural Philippine electric co-operatives were trained to provide regular maintenance and it is planned that several of the system components will be sourced or manufactured in the Philippines. Most of the system components will be rented to the Philippine families for a monthly fee. To promote self-maintenance, each family will own its system battery. (UK)

  18. First report of Tomato chlorosis virus infecting sweet pepper in Costa Rica

    Science.gov (United States)

    In September 2008, a survey of whiteflies and whitefly-borne viruses was performed in greenhouses in the province of Cartago, Costa Rica. During this survey, sweet pepper (Capsicum annuum cv. Nataly) plants showing interveinal chlorosis, enations, necrosis, and mild upward leaf curling were observed...

  19. Behavior of the Linea Alba During a Curl-up Task in Diastasis Rectus Abdominis: An Observational Study.

    Science.gov (United States)

    Lee, Diane; Hodges, Paul W

    2016-07-01

    Study Design Cross-sectional repeated measures. Background Rehabilitation of diastasis rectus abdominis (DRA) generally aims to reduce the inter-rectus distance (IRD). We tested the hypothesis that activation of the transversus abdominis (TrA) before a curl-up would reduce IRD narrowing, with less linea alba (LA) distortion/deformation, which may allow better force transfer between sides of the abdominal wall. Objectives This study investigated behavior of the LA and IRD during curl-ups performed naturally and with preactivation of the TrA. Methods Curl-ups were performed by 26 women with DRA and 17 healthy control participants using a natural strategy (automatic curl-up) and with TrA preactivation (TrA curl-up). Ultrasound images were recorded at 2 points above the umbilicus (U point and UX point). Ultrasound measures of IRD and a novel measure of LA distortion (distortion index: average deviation of the LA from the shortest path between the recti) were compared between 3 tasks (rest, automatic curl-up, TrA curl-up), between groups, and between measurement points (analysis of variance). Results Automatic curl-up by women with DRA narrowed the IRD from resting values (mean U-point between-task difference, -1.19 cm; 95% confidence interval [CI]: -1.45, -0.93; PUX-point between-task difference, -0.51 cm; 95% CI: -0.69, -0.34; PUX-point between-task difference, 0.025; 95% CI: 0.004, 0.045; P = .02). Although TrA curl-up induced no narrowing or less IRD narrowing than automatic curl-up (mean U-point difference between TrA curl-up versus rest, -0.56 cm; 95% CI: -0.82, -0.31; PUX-point between-task difference, 0.02 cm; 95% CI: -0.22, 0.19; P = .86), LA distortion was less (mean U-point between-task difference, -0.025; 95% CI: -0.037, -0.012; PUX-point between-task difference, -0.021; 95% CI: -0.038, -0.005; P = .01). Inter-rectus distance and the distortion index did not change from rest or differ between tasks for controls (P≥.55). Conclusion Narrowing of the IRD

  20. Curl flux, coherence, and population landscape of molecular systems: Nonequilibrium quantum steady state, energy (charge) transport, and thermodynamics

    CERN Document Server

    Zhang, Zhedong

    2015-01-01

    We established a theoretical framework in terms of the curl flux, population landscape, and coherence for non-equilibrium quantum systems at steady state, through exploring the energy and charge transport in molecular processes. The curl quantum flux plays the key role in determining transport properties and the system reaches equilibrium when flux vanishes. The novel curl quantum flux reflects the degree of non-equilibriumness and the time-irreversibility. We found an analytical expression for the quantum flux and its relationship to the environmental pumping (non-equilibriumness quantified by the voltage away from the equilibrium) and the quantum tunneling. Furthermore, we investigated another quantum signature, the coherence, quantitatively measured by the non-zero off diagonal element of the density matrix. Besides the environment-assistance which can give dramatic enhancement of coherence and quantum flux with high voltage at a fixed tunneling strength, the quantum flux is promoted by the coherence in th...

  1. 77 FR 40329 - U.S. Renewable Energy Trade Mission Philippines and Thailand, Manila, Philippines and Bangkok...

    Science.gov (United States)

    2012-07-09

    ... web sites, press releases to general and trade media, direct mail, notices by industry trade... International Trade Administration U.S. Renewable Energy Trade Mission Philippines and Thailand, Manila, Philippines and Bangkok, Thailand, September 17-20, 2012 AGENCY: International Trade Administration...

  2. Epidemiology of cholera in the Philippines.

    Directory of Open Access Journals (Sweden)

    Anna Lena Lopez

    2015-01-01

    Full Text Available Despite being a cholera-endemic country, data on cholera in the Philippines remain sparse. Knowing the areas where cholera is known to occur and the factors that lead to its occurrence will assist in planning preventive measures and disaster mitigation.Using sentinel surveillance data, PubMed and ProMED searches covering information from 2008-2013 and event-based surveillance reports from 2010-2013, we assessed the epidemiology of cholera in the Philippines. Using spatial log regression, we assessed the role of water, sanitation and population density on the incidence of cholera.We identified 12 articles from ProMED and none from PubMed that reported on cholera in the Philippines from 2008 to 2013. Data from ProMed and surveillance revealed 42,071 suspected and confirmed cholera cases reported from 2008 to 2013, among which only 5,006 were confirmed. 38 (47% of 81 provinces and metropolitan regions reported at least one confirmed case of cholera and 32 (40% reported at least one suspected case. The overall case fatality ratio in sentinel sites was 0.62%, but was 2% in outbreaks. All age groups were affected. Using both confirmed and suspected cholera cases, the average annual incidence in 2010-2013 was 9.1 per 100,000 population. Poor access to improved sanitation was consistently associated with higher cholera incidence. Paradoxically, access to improved water sources was associated with higher cholera incidence using both suspected and confirmed cholera data sources. This finding may have been due to the breakdown in the infrastructure and non-chlorination of water supplies, emphasizing the need to maintain public water systems.Our findings confirm that cholera affects a large proportion of the provinces in the country. Identifying areas most at risk for cholera will support the development and implementation of policies to minimize the morbidity and mortality due to this disease.

  3. People first in the Philippines: Gelia Castillo.

    Science.gov (United States)

    Yabes, C

    1995-01-01

    Gelia Castillo, a specialist in rural sociology whose ideas have influenced policymakers in government, international development circles, and academe, pioneered the concept of participatory development. Born into a poor family, her father convinced her that academic achievement was the key to her future; her academic training includes a Bachelor of Arts in psychology from the University of the Philippines, a Master's in rural sociology from Pennsylvania State University, and a Doctorate from Cornell University. She served as professor of rural sociology at the College of Agriculture, University of the Philippines, and published extensively on women's roles, the sex roles of Filipino adolescents, agricultural school administration, the team approach in community development, rice and potato farming, the changing social images in a developing society, and the protein gap. "Beyond Manila," her most famous book, was the first comprehensive research work on income distribution, employment, labor, education, and migration in the rural Philippines. She underscored the definition of "household" and observed that the role of women and children contributes greatly to the dynamics of society. Her husband, Leopoldo Castillo, an animal nutritionist who is now professor emeritus at the Institute of Animal Science, has long supported his wife's career. The mother of two daughters and one son was the only female member of the board of the International Potato Center for 6 years and served on the boards of the IDRC, the International Service for National Agriculture Research, and the International Council for Research in Agroforestry. At 66, and retired, she believes affirmative action is necessary and that the feminist movement has only helped professional women.

  4. Epidemiology of Cholera in the Philippines

    Science.gov (United States)

    Lopez, Anna Lena; Macasaet, Lino Y.; Ylade, Michelle; Tayag, Enrique A.; Ali, Mohammad

    2015-01-01

    Background Despite being a cholera-endemic country, data on cholera in the Philippines remain sparse. Knowing the areas where cholera is known to occur and the factors that lead to its occurrence will assist in planning preventive measures and disaster mitigation. Methods Using sentinel surveillance data, PubMed and ProMED searches covering information from 2008–2013 and event-based surveillance reports from 2010–2013, we assessed the epidemiology of cholera in the Philippines. Using spatial log regression, we assessed the role of water, sanitation and population density on the incidence of cholera. Results and Discussion We identified 12 articles from ProMED and none from PubMed that reported on cholera in the Philippines from 2008 to 2013. Data from ProMed and surveillance revealed 42,071 suspected and confirmed cholera cases reported from 2008 to 2013, among which only 5,006 were confirmed. 38 (47%) of 81 provinces and metropolitan regions reported at least one confirmed case of cholera and 32 (40%) reported at least one suspected case. The overall case fatality ratio in sentinel sites was 0.62%, but was 2% in outbreaks. All age groups were affected. Using both confirmed and suspected cholera cases, the average annual incidence in 2010–2013 was 9.1 per 100,000 population. Poor access to improved sanitation was consistently associated with higher cholera incidence. Paradoxically, access to improved water sources was associated with higher cholera incidence using both suspected and confirmed cholera data sources. This finding may have been due to the breakdown in the infrastructure and non-chlorination of water supplies, emphasizing the need to maintain public water systems. Conclusion Our findings confirm that cholera affects a large proportion of the provinces in the country. Identifying areas most at risk for cholera will support the development and implementation of policies to minimize the morbidity and mortality due to this disease. PMID:25569505

  5. Toxicity of thiamethoxam against Philippine subterranean termites.

    Science.gov (United States)

    Acda, Menandro N

    2007-01-01

    Thiamethoxam (ACTARA 25WG) was evaluated for its termiticidal properties against three species of economically important subterranean termites (Isoptera: Termitidae) in the Philippines: Nasutitermes luzonicus Oshima, Macrotermes gilvus Hagen, and Microcerotermes losbanosensis Oshima. Results of the study indicated that exposure to soil or ingestion of paper treated with thiamethoxam at concentration above 0.41 ppm may provide an adequate chemical barrier or induce high mortality against N. luzonicus, M. gilvus and M. losbanosensis after 5-9 days. Feeding bioassays showed that thiamethoxam was not repellent to M. gilvus and M. losbanosensis but had an anti-feeding effect on N. luzonicus.

  6. Profile: the Philippine Population Information Network.

    Science.gov (United States)

    1991-06-01

    The profile of Philippine Population Information Network (POPIN) is described in this article as having changed management structure from the Population Center Foundation to the Government's Population Commission, Information Management and Research Division (IMRD) in 1989. This restructuring resulted in the transfer in 1990 of the Department of Social Welfare and Development to the Office of the President. POPIN also serves Asia/Pacific POPIN. POPCOM makes policy and coordinates and monitors population activities. POPIN's goal is to improve the flow and utilization of population information nationwide. The National Population Library was moved in 1989 to the POPCOM Central Office Building and became the Philippine Information Center. The collection includes 6000 books, 400 research reports, and 4000 other documents (brochures, reprints, conference materials, and so on); 42 video tapes about the Philippine population program and a cassette player are available. In 1989, 14 regional centers were set up in POPCOM regional offices and designated Regional Population Information Centers. There are also school-based information centers operating as satellite information centers. The Regional and school-based centers serve the purpose of providing technical information through collection development, cataloguing, classification, storage and retrieval, and circulation. The target users are policy makers, government and private research agencies, researchers, and faculty and students. Publications developed and produced by the Center include the 3rd Supplement of the Union Catalogue of Population Literature, the 1987-88 Annotated Bibliography of Philippine Population Literature (PPL), the forthcoming 1989-90 edition of the Annotated Bibliography of PPL, and a biyearly newsletter, POPINEWS. Microcomputers have been acquired for the Regional Centers, with the idea of computerizing POPIN. Computer upgrading is also being done within the IMRD to provide POPLINE CD

  7. The real stroke burden in the Philippines.

    Science.gov (United States)

    Navarro, Jose C; Baroque, Alejandro C; Lokin, Johnny K; Venketasubramanian, Narayanaswamy

    2014-07-01

    Stroke is the Philippines' second leading cause of death. It has a prevalence of 0·9%; ischemic stroke comprises 70% while hemorrhagic stroke comprises 30%. Age-adjusted hypertension prevalence is 20·6%, diabetes 6·0%, dyslipidemia 72·0%, smoking 31%, and obesity 4·9%. The neurologist-to-patient ratio is 1:330·000, with 67% of neurologists practicing in urban centers. Health care is largely private and the cost is borne out-of-pocket by patients and their families. Challenges include delivering adequate support to the rural communities and to the underprivileged sectors.

  8. Philippines: Small-scale renewable energy update

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-01

    This paper gives an overview of the application of small scale renewable energy sources in the Philippines. Sources looked at include solar, biomass, micro-hydroelectric, mini-hydroelectric, wind, mini-geothermal, and hybrid. A small power utilities group is being spun off the major utility, to provide a structure for developing rural electrification programs. In some instances, private companies have stepped forward, avoiding what is perceived as overwhelming beaurocracy, and installed systems with private financing. The paper provides information on survey work which has been done on resources, and the status of cooperative programs to develop renewable systems in the nation.

  9. Wave control through soft microstructural curling: bandgap shifting, reconfigurable anisotropy and switchable chirality

    Science.gov (United States)

    Celli, Paolo; Gonella, Stefano; Tajeddini, Vahid; Muliana, Anastasia; Ahmed, Saad; Ounaies, Zoubeida

    2017-03-01

    In this work, we discuss and numerically validate a strategy to attain reversible macroscopic changes in the wave propagation characteristics of cellular metamaterials with soft microstructures. The proposed cellular architecture is characterized by unit cells featuring auxiliary populations of symmetrically-distributed smart cantilevers stemming from the nodal locations. Through an external stimulus (the application of an electric field), we induce extreme, localized, reversible curling deformation of the cantilevers—a shape modification which does not affect the overall shape, stiffness and load bearing capability of the structure. By carefully engineering the spatial pattern of straight (non activated) and curled (activated) cantilevers, we can induce several profound modifications of the phononic characteristics of the structure: generation and/or shifting of total and partial bandgaps, cell symmetry relaxation (which implies reconfigurable wave beaming), and chirality switching. While in this work we discuss the specific case of composite cantilevers with a PDMS core and active layers of electrostrictive terpolymer P(VDF-TrFE-CTFE), the strategy can be extended to other smart materials (such as dielectric elastomers or shape-memory polymers).

  10. Radiographic inguinal curl may indicate paraspinal misplacement of percutaneously inserted central venous catheters: report of three cases

    Energy Technology Data Exchange (ETDEWEB)

    Chedid, Faris [Al Tawam Hospital, Department of Paediatrics, Al Ain, Abu Dhabi (United Arab Emirates); Abbas, Adil [Royal Children' s Hospital, Department of Clinical Haematology/Oncology, Brisbane (Australia); Morris, Lloyd [Women' s and Children' s Hospital, Department of Medical Imaging, Adelaide (Australia)

    2005-07-01

    Misplacement of percutaneously inserted central venous catheters (PCVCs) into the paraspinal venous plexus can result in devastating outcomes. Several cases have been reported in the literature together with an explanation of the mechanism. To describe three premature babies with their PCVCs inserted through the left saphenous vein that ended up in the lumbar spinal dural venous plexus. Plain radiographs obtained to check positions showed an unusual 360 curl of the PCVC in the left inguinal area. We believe that misplacement of the catheter into the paraspinal venous plexus could be diagnosed with great accuracy if such a curl is seen. (orig.)

  11. Ixora (Rubiaceae) on the Philippines - crossroad or cradle?

    Science.gov (United States)

    Banag, Cecilia I; Mouly, Arnaud; Alejandro, Grecebio Jonathan D; Bremer, Birgitta; Meve, Ulrich; Grimm, Guido W; Liede-Schumann, Sigrid

    2017-06-07

    The Philippine archipelago is globally one of the most important model island systems for studying evolutionary processes. However, most plant species on this archipelago have not yet been studied in sufficient detail. The main aim of this study is to unravel the evolutionary history and biogeographic relationships of the Philippine members of the pantropical genus Ixora. The complex plastid and nuclear divergence patterns in Philippine Ixora, documented using tree and network approaches, reveal a highly dynamic evolution in Ixora, involving several phases of radiation and recolonization. Philippine Ixora comprises at least five lineages, of which one is most closely related to species from Wallacea, and the remaining four to species from Asia. Our study highlights the importance of Philippine species for understanding phytogeographic patterns in the Indomalayan-Australasian eco-region. The overall genetic differentiation, as well as the incongruence between genealogies based on the biparentally inherited nucleome and the maternally inherited plastome in Ixora, reflect the complex tectonic history of the Philippine archipelago. The Ixora lineage related to Wallacean species supports the delimitation of different ecozones along Huxley's line, because it is absent from Palawan. The remaining four lineages are all allied with Asian taxa, reflecting several waves of colonization. Close relationships between some widespread Philippine species and locally adapted narrow endemics suggest that the widespread, genetically diverse species act as pools for the formation of new species in a process of ongoing speciation. Our results suggest that the species concepts of some of the more widespread taxa need to be revised.

  12. Philippines to seek Thai assistance in fighting AIDS.

    Science.gov (United States)

    A group of Philippine officials left for Thailand to learn from the Thai government's experience in fighting AIDS. Thailand has one of the highest AIDS levels in Asia and has instituted a variety of public and private programs to deal with the disease. Health Undersecretary Carmencita Reodica, who is leading the delegation, said the Philippines does not expect as many AIDS cases as Thailand. "But if the Philippines does not improve its planning and surveillance system, we could face the same threat in the future," she said. Thailand has a good surveillance program, valuable support services such as mobile clinics, and active private groups fighting AIDS, she said. The group is to meet with Prime Minister Banharn Silpa-archa, who heads the Thai National AIDS Council, and other officials involved in Thailand's anti-AIDS campaign. Thailand has recorded 22,135 AIDS cases and 750,000 people with HIV. In comparison, the Philippine government has documented only 234 AIDS cases in the Philippines and 704 people with HIV. Health officials estimate that as many as 35,000 people in the country are infected with HIV. While Thailand monitors the number of AIDS cases in the general population, the Philippines only monitors the disease among so-called high-risk groups such as prostitutes, homosexuals, and intravenous drug users. Reodica said legislation is needed in the Philippines to support the country's AIDS program, as well as to answer ethical and legal issues concerning AIDS cases. full text

  13. Electricity industry reforms in the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    Sharma, Deepak E-mail: deepak.sharma@uts.edu.au; Madamba, S.E.; Chan, M.R.L

    2004-09-01

    The privatization of the electricity industry appears to be the leitmotif of the Philippine electricity reform. The restructuring of the industry is expected to make it malleable enough for the private sector to then mould it--with support from pro-private-market regulation--into an efficient enterprise. This, the argument continues, will have a vivifying effect on all spheres of the economy. A deeper review of the proposed reform program however suggests that much of the underlying argumentation relating to the why and how of the reform program is untenable. It is based largely on perceptions and unfounded beliefs rather than reality and meaningful analysis. This could result in the obfuscation of real challenges confronting the Philippine electricity industry and preclude consideration of meaningful alternatives to improve industry performance. The broad contours of such alternatives could include competitive sourcing of new generation capacity, publicly owned transmission and distribution networks, a regulated retail segment and responsive regulatory and governance arrangements capable of conciliating the aspirations of various interests groups in a transparent and efficacious manner.

  14. Population planning policy in the Philippines.

    Science.gov (United States)

    Flavier, J M

    1973-01-01

    Population policy of the Philippine government is discussed in its evolution, current status under the 1973 constitution, and future development. Despite the low income, strong family ties, 7000 islands, 87 languages and 82% Catholic population, the Philippines has an official population policy which evolved into the Population Commission in 1969. The commission recommended setting goals, furthering education, removing legal drawbacks, reducing mortality, regulating internal migration and coordinating public and private international funding organizations. The private organizations, international funding,mass media, academic influence, and possibly the Catholic hierarchy influenced events positively. During martial law (September 1972 to January 1973) the government took on responsibility to require licensed professionals to learn family planning, and to change tax exemptions. The constitution now states that the State must achieve a population level conducive to national welfare. The present policy considers population planning an economic priority, has placed 5 cabinet members on the board of the Population Commission (no representatives from private organizations, which recruit 70% of acceptors), and has an extremely optimistic 5 year plan. No public protest had emerged, and because rhythm is included, it is hoped that rhythm users well eventually change to effective methods. Foreign assistance will be needed, and is channeled through the National Economic and Development Authority. Future needs include paramidics, nonclinical methods, rural access, incentives for program workers, and more effective propaganda.

  15. Links between the Philippines and Spain: migration and bilateral relations

    Directory of Open Access Journals (Sweden)

    Rogelia Pe-Pua

    2005-01-01

    Full Text Available This paper analyses the implications which Spanish policy regarding foreign workers has on the living and working conditions of the Filipino community in Spain. The author pays special attention to bilateral relations between the Philippines and Spain in issues suchas Spanish investment in the Philippines, the trade balance between the two countries and labour relations. In conclusion the article considers the necessity of reaching a bilateral labour agreement which would be beneficial to both countries and which at the same time would improve the working conditions and the integration of Philippine nationals living in Spain.

  16. Status of radiation education and training in the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    Bernido, C.C. [Philippine Nuclear Research Institute, Commonwealth Avenue, Diliman, Quezon (Philippines)

    1999-09-01

    There are three major sources and levels of obtaining radiation or nuclear education and training in the Philippines: the secondary schools or high schools; colleges and universities; and training courses in nuclear science and radiation protection offered by government agencies such as the Philippine Nuclear Research Institute (PNRI) of the Department of Science and Technology and the Radiation Health Service (RHS) of the Department of Health. This paper summarizes the status, some of the activities and some of the problems of radiation education in the Philippines. (author)

  17. Core Muscle Activation During Unstable Bicep Curl Using a Water-Filled Instability Training Tube.

    Science.gov (United States)

    Glass, Stephen C; Blanchette, Taylor W; Karwan, Lauren A; Pearson, Spencer S; OʼNeil, Allison P; Karlik, Dustin A

    2016-11-01

    Glass, SC, Blanchette, TW, Karwan, LA, Pearson, SS, O'Neil, AP, and Karlik, DA. Core muscle activation during unstable bicep curl using a water-filled instability training tube. J Strength Cond Res 30(11): 3212-3219, 2016-The purpose of this study was to assess compensatory muscle activation created during a bicep curl using a water-filled, unstable lifting tube. Ten men (age = 21 ± 1.6 years, height = 180.0 ± 3.3 cm, mass = 87.4 ± 15.0 kg) and 10 women (age = 19.6 ± 1.3 years, height = 161.4 ± 12.0 cm, mass = 61.2 ± 7.4 kg) completed bicep curls using an 11.4-kg tube partially filled with water during a 50% open-valve, 100% open, and control setting. Subjects completed 8 repetitions within each condition with integrated electromyographic signal (converted to percent maximal voluntary contraction) of the bicep, deltoid, rectus abdominus, and paraspinal muscles measured. Compensatory activation was determined using the natural log of coefficient of variation across concentric (CON) and eccentric (ECC) contractions. There were no differences between gender for any condition. Significant variability was seen across treatments for paraspinal muscles for CON and ECC at 50% (CON LnCV = 3.13 ± 0.56%, ECC LnCV = 3.34 ± 0.58%) and 100% (CON = 3.24 ± 0.34%, ECC = 3.46 ± 0.35%) compared with control (CON = 2.59 ± 0.47%, ECC = 2.80 ± 0.61%). Deltoid variability was greater at the 100% open setting (CON = 3.51 ± 0.53%, ECC = 3.56 ± 0.36%) compared with control (CON = 2.98 ± 0.35%, ECC = 2.97 ± 0.45%). The abdominal CON 100% showed variability (3.02 ± 0.47%) compared with control (2.65 ± 0.43%). Bicep activation remained unvaried. Compensatory activation of postural muscles contribute to postural stability. This device may be a useful tool for neuromuscular training leading to improved stability and control.

  18. Developing the Philippines as a Global Hub for Disaster Risk Reduction - A Health Research Initiative as Presented at the 10th Philippine National Health Research System Week Celebration

    OpenAIRE

    Banwell, Nicola; Montoya, Jaime; Opeña, Merlita; IJsselmuiden, Carel; Law, Ronald; Balboa, Gloria J.; Rutherford, Shannon; Chu, Cordia; Murray, Virginia

    2016-01-01

    The recent Philippine National Health Research System (PNHRS) Week Celebration highlighted the growing commitment to Disaster Risk Reduction (DRR) in the Philippines. The event was lead by the Philippine Council for Health Research and Development of the Department of Science and Technology and the Department of Health, and saw the participation of national and international experts in DRR, and numerous research consortia from all over the Philippines. With a central focus on the Sendai Frame...

  19. Nod factor-induced root hair curling: continuous polar growth towards the point of nod factor application

    NARCIS (Netherlands)

    Esseling, J.J.; Lhuissier, F.G.P.; Emons, A.M.C.

    2003-01-01

    A critical step in establishing a successful nitrogen-fixing symbiosis between rhizobia and legume plants is the entrapment of the bacteria between root hair cell walls, usually in characteristic 180degrees to 360degrees curls, shepherd's crooks, which are formed by the host's root hairs. Purified b

  20. Occurrence and distribution of pepper veinal mottle virus and cucumber mosaic virus in pepper in Ibadan, Nigeria

    Directory of Open Access Journals (Sweden)

    Arogundade Olawale

    2012-04-01

    Full Text Available Abstract Viral diseases constitute obstacles to pepper production in the world. In Nigeria, pepper plants are primarily affected by pepper veinal mottle virus (PVMV, Cucumber mosaic virus (CMV, Pepper leaf curl Virus (TLCV, Tobacco mosaic virus (TMV, Pepper mottle virus (PMV and a host of other viruses. The experiment was carried out with a diagnostic survey on the experimental field of the National Horticultural Research Institute, Ibadan, Nigeria and on pepper farms in six local government areas within Ibadan Oyo State, Nigeria, forty samples were collected from each of the farms. Diseased samples were obtained from the field and taken to the laboratory for indexing. In ELISA test some of the samples from the pepper farms showed positive reaction to single infection with PVMV (36.79%, CMV (22.14% while some others showed positive reaction to mixed infection of the two viruses (10% but some also negative reaction to PVMV and CMV antisera (31.07.

  1. REDD+ in the Philippines: Legal status and conservation of mangrove forests in the Philippines

    Directory of Open Access Journals (Sweden)

    Gloria Estenzo Ramos

    2013-07-01

    Full Text Available Mangroves perform a crucial role in maintaining the ecological integrity of the coastal ecosystem. They  act as filters in the coastal zone, preventing the damaging effects of upland sediments on seagrass beds and coral reefs, minimise the effects of storm surges and act as carbon sinks that mitigate climate change. These essential services, however, are degraded through indiscriminate cutting, conversion of mangrove swamps to fishponds, reclamation projects and other coastal developments and pollution. Experts reveal that the Indo-Malay Philippine Archipelago has one of the highest rates of mangroves loss. From an estimated 500,000 hectares of mangrove cover in 1918, only 120,000 hectares of mangroves remain in the Philippines today. The country has had the legal and policy framework to protect and conserve mangroves. But weak implementation of laws, overlapping functions among agencies and, in general, poor management by the people and local governments have hindered the sustainable management of mangrove forests. Positive developments, however, are taking place with the promulgation of laws on climate change and executive orders which specifically include mangrove and protected areas under the National Greening Program (NGP and addresses equity, food security and poverty issues by giving preference to NGP beneficiary communities as a priority in the Conditional Cash Transfer (CCT Program.  Moreover, participatory Planning and Multi-stakeholder Approaches are among the strategies contemplated by the Philippine National REDD + Strategy. The article examines the implementation and effects of the Philippine National REDD+ Strategy, the National Climate Change Action Plan which specifically integrates REDD+ and ecosystem valuation into decision-making, and the executive orders which support the mainstreaming of the National Greening Program.

  2. Human-Mediated Marine Dispersal Influences the Population Structure of Aedes aegypti in the Philippine Archipelago.

    Directory of Open Access Journals (Sweden)

    Eugenio Fonzi

    Full Text Available Dengue virus (DENV is an extraordinary health burden on global scale, but still lacks effective vaccine. The Philippines is endemic for dengue fever, but massive employment of insecticides favored the development of resistance mutations in its major vector, Aedes aegypti. Alternative vector control strategies consist in releasing artificially modified mosquitos in the wild, but knowledge on their dispersal ability is necessary for a successful implementation. Despite being documented that Ae. aegypti can be passively transported for long distances, no study to date has been aimed at understanding whether human marine transportation can substantially shape the migration patterns of this mosquito. With thousands of islands connected by a dense network of ships, the Philippines is an ideal environment to fill this knowledge gap.Larvae of Ae. aegypti from 15 seaports in seven major islands of central-western Philippines were collected and genotyped at seven microsatellite loci. Low genetic structure and considerable gene flow was found in the area. Univariate and multivariate regression analyses suggested that anthropic factors (specifically the amount of processed cargo and human population density can explain the observed population structure, while geographical distance was not correlated. Interestingly, cargo shipments seem to be more efficient than passenger ships in transporting Ae. aegypti. Bayesian clustering confirmed that Ae. aegypti from busy ports are more genetically similar, while populations from idle ports are relatively structured, regardless of the geographical distance that separates them.The results confirmed the pivotal role of marine human-mediated long-range dispersal in determining the population structure of Ae. aegypti. Hopefully corroborated by further research, the present findings could assist the design of more effective vector control strategies.

  3. World Rabies Day campaign in the Philippines.

    Science.gov (United States)

    Medina, Danellie Joy O; Jayme, Sarah I; Amparo, Anna Charinna B; Cresencio, Rubina O; Lopez, Emelinda L; Baquilod, Mario S; Hernandez, Leda M; Villalon, Ernesto E S; Nel, Louis D

    2016-01-01

    Rabies is a fatal disease, claiming the lives of around 59,000 people annually worldwide. It is considered a neglected and underreported disease leading to inadequate support from governments. Apart from dog vaccination and proper animal bite management, an integral part of a successful rabies control program is community education. The Philippine government conducts an extensive nationwide annual World Rabies Day (WRD) celebration as part of its community education. Strong inter-sectoral collaboration at the national level is a key factor for the success of WRD, capitalizing on the partners' strengths to mobilize various sectors. Strategies include the National WRD Celebration and releasing national government memorandums. An invitation letter campaign was initiated, encouraging stakeholders to register their activities. Banners were given as an incentive for those who registered. Mass and social media were also utilized to promote WRD. Registered WRD events held in the Philippines rose from 10 events in 2012, to 37 events in 2013, to 66 events in 2014 and 76 events in 2015. The individual activities involved veterinary services and information, communication, and education (IEC) activities. Nine unique WRD IEC activities are highlighted in this paper. Promotion of WRD through social media was also utilized in recent years. More news items were published online than those printed in newspapers and aired on television. The campaign's success underlines the value of a national government-led program. The national rabies program sets the agenda for priority activities including the WRD campaign. Its capacity to allocate funds for the program also denotes stability which is beneficial for local program implementers. Different segments of society were tapped through various strategies. The campaign's flexibility allowed for a large range of activities and presented opportunities for expanding partnerships and integration with others interventions for its sustainability

  4. Parallel eigensolver for H(curl) problems using H1-auxiliary space AMG preconditioning

    Energy Technology Data Exchange (ETDEWEB)

    Kolev, T V; Vassilevski, P S

    2006-11-15

    This report describes an application of the recently developed H{sup 1}-auxiliary space preconditioner for H(curl) problems to the Maxwell eigenvalue problem. The auxiliary space method based on the new (HX) finite element space decomposition introduced in [7], was implemented in the hypre library, [10, 11] under the name AMS. The eigensolver considered in the present paper, referred to as the AME, is an extension of the AMS. It is based on the locally optimal block eigensolver LOBPCG [9] and the parallel AMG (algebraic multigrid) solver BoomerAMG [2] from the hypre library. AME is designed to compute a block of few minimal nonzero eigenvalues and eigenvectors, for general unstructured finite element discretizations utilizing the lowest order Nedelec elements. The main goal of the current report is to document the usage of AME and to illustrate its parallel scalability.

  5. Effect of Deformation Condition on Axial CompressivePrecision Forming Process of Tube with Curling Die

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The effect of the deformation condition on the axial compressive precision forming process of tube with curling die was investigated by using a rigid-plastic FEM. The results show that the forming accuracy depends mainly on geometric condition rρ/d0, little on tube material properties and friction condition; the relative gap Δ/2rρ of double-walled tubes obtained decreases with increasing rρ/d0, and there is a parameter k for a given t0/d0 or rρ/t0, when rρ,/d0>k, Δ/2rρ<1,otherwise Δ/2rρ>1.

  6. Simple and Accurate Analytical Solutions of the Electrostatically Actuated Curled Beam Problem

    KAUST Repository

    Younis, Mohammad I.

    2014-08-17

    We present analytical solutions of the electrostatically actuated initially deformed cantilever beam problem. We use a continuous Euler-Bernoulli beam model combined with a single-mode Galerkin approximation. We derive simple analytical expressions for two commonly observed deformed beams configurations: the curled and tilted configurations. The derived analytical formulas are validated by comparing their results to experimental data in the literature and numerical results of a multi-mode reduced order model. The derived expressions do not involve any complicated integrals or complex terms and can be conveniently used by designers for quick, yet accurate, estimations. The formulas are found to yield accurate results for most commonly encountered microbeams of initial tip deflections of few microns. For largely deformed beams, we found that these formulas yield less accurate results due to the limitations of the single-mode approximations they are based on. In such cases, multi-mode reduced order models need to be utilized.

  7. Enhancement of lesion classification using divergence, curl and curvature of skin pattern.

    Science.gov (United States)

    She, Zhishun; Duller, A W G; Fish, P J

    2004-11-01

    The observation that skin pattern tends to be disrupted by malignant but not by benign skin lesions suggests that measurements of skin pattern disruption on simply captured white light optical clinical (WLC) skin images could be a useful contribution to a diagnostic feature set. Previous work which generated a flow field of skin pattern using a measurement of local line direction and variation determined by the minimum eigenvalue and its corresponding eigenvector of the local tensor matrix to measure skin pattern disruption was computationally low cost and encouraging. This paper explores the possibility of extracting new features from the first and second differentiations of this flow field to enhance classification performance. The skin pattern was extracted from WLC skin images by high-pass filtering. The skin line direction was estimated using a local image gradient matrix to produce a flow field of skin pattern. Divergence, curl, mean and Gaussian curvatures of this flow field were computed from the first and second differentiations of this flow field. The difference of these measures combined with skin line direction across the lesion image boundary was used as a lesion classifier. A set of images of malignant melanoma and benign naevi were analysed as above and the scatter plot in a two-dimensional dominant feature space using principal component analysis showed excellent separation of benign and malignant lesions. A receiver operating characteristic plot enclosed an area of 0.96. The experimental results show that the divergence, curl, mean and Gaussian curvatures of the flow field can increase lesion classifier accuracy. Combined with skin line direction they are promising features for distinguishing malignant melanoma from benign lesions and the methods used are computationally efficient which is important if their use is to be considered in clinical practice.

  8. Population-Development-Environment Modeling in the Philippines: A Review

    OpenAIRE

    1996-01-01

    This article surveys existing forecasting models in the Philippines and discusses several promising alternatives in the process of developing a methodological modeling. Investigation of CGE models leads to the findings of the absence of population-environment interactions.

  9. Philippine Public Relations: An Industry and Practitioner Profile.

    Science.gov (United States)

    Panol, Zenaida Sarabia

    2000-01-01

    Provides a comprehensive look at public relations in the Philippines; traces how public relations evolved in this Southeast Asian country; and discusses the current status of the industry and its practitioners. (NH)

  10. Nepenthes samar (Nepenthaceae), a new species from Samar, Philippines

    NARCIS (Netherlands)

    Cheek, M.; Jebb, M.

    2013-01-01

    Nepenthes samar is described from the island of Samar in the Visayas of the Philippines. Similar to N. merrilliana of Mindanao, it is distinguished on foliar and floral characteristics and assessed as Critically Endangered using the IUCN 2001 standard.

  11. The Northern Philippine Sea: A Bioregional Development Communication Strategy

    Directory of Open Access Journals (Sweden)

    Marivic G. Pajaro

    2013-12-01

    Full Text Available The Philippine marine management challenge requires a scaled up ecosystem approach to the biodiversity-based bioregional level used in marine spatial planning. The related communication challenge is being addressed by a currently informal consortium that includes non-government organizations, local government units, as well as state colleges and universities. The evolving communication strategy described here is focused upon considerations that include local government mandates, status of marine development, provinceby- province assessment of coastal economies, cultural relevance, academic programming, and the need for national inputs on counterpart funding. The current work provides a possible model for Philippine application in all marine bioregions. The concept of the bioregional approach was systematically advocated across one bioregion, the Northern Philippine Sea. The Philippine strategy of development communication was used as a template to promote the development of a bioregional approach by establishing an initial level of participation involving the provincial governments as well as the state universities and colleges.

  12. Nepenthes samar (Nepenthaceae), a new species from Samar, Philippines

    NARCIS (Netherlands)

    Cheek, M.; Jebb, M.

    2013-01-01

    Nepenthes samar is described from the island of Samar in the Visayas of the Philippines. Similar to N. merrilliana of Mindanao, it is distinguished on foliar and floral characteristics and assessed as Critically Endangered using the IUCN 2001 standard.

  13. Hydrodynamics, sediment transport and light extinction off Cape Bolinao, Philippines

    NARCIS (Netherlands)

    Rivera, P.C.

    1997-01-01

    Observational and numerical modelling studies of the hydrodynamics, sediment transport, and light extinction were undertaken in the marine environment around Cape Bolinao in the Lingayen Gulf (Northwest Philippines). Abundant with ecologically important seagrasses and benthic organisms,

  14. Tradition, Misconception, and Contribution: Chinese Influences in Philippine Culture

    Directory of Open Access Journals (Sweden)

    Maningning C. Miclat

    2000-12-01

    Full Text Available This paper discusses Chinese influence on Philippine arts and crafts, as shown in artifacts from the Sino-Philippine trade of pre-Hispanic times-the churches, religious icons, and paintings of the Spanish period-and in the contemporary art of the Chinese Filipinos. The Chinese traditional elements are given new meanings in a new environment, and it is these misconceptions and misinterpretations of the imported concepts that influence and enrich our culture.

  15. Transvestites and transgressions: Panggagaya in Philippine gay cinema.

    Science.gov (United States)

    Tolentino, R B

    2000-01-01

    Philippine cinema has long afforded spectators constructions of queerness not only deviating from Western queer theory but also from other continentally Asian homosexual traditions. Using as my primary text Lino Brocka's 1978 film My Father, My Mother, I examine how indigenous ideas of transsexuality and transgender are not only constructed by alternatively sexual images manufactured and appropriated by the Marcos regime, but how those sexual images construct and perpetuate class divisions as criteria of an ongoing Philippine sexual economy.

  16. Nematode problems affecting agriculture in the Philippines.

    Science.gov (United States)

    Davide, R G

    1988-04-01

    Nematodes are considered major pests on most economic crops in the Philippines, particularly on banana, pineapple, citrus, tomato, ramie, and sugarcane. Radopholus similis is the most destructive nematode on banana, while Meloidogyne spp. are more serious on various vegetable crops such as tomato, okra, and celery and on fiber crops such as ramie. Tylenchulus semipenetrans is a problem on citrus and Rotylenchulus reniformis on pineapple and some legume crops. Hirschmanniella oryzae and Aphelenchoides besseyi are becoming serious on rice, and Pratylenchus zeae is affecting corn in some areas. Lately, Globodera rostochiensis has been causing serious damage on potato in the highlands. Control measures such as crop rotation, planting resistant varieties, chemical nematicide application, and biological control have been recommended to control these nematodes.

  17. Independent focuses Philippines exploration on Visayan basin

    Energy Technology Data Exchange (ETDEWEB)

    Rillera, F.G. [Cophil Exploration Corp., Manila (Philippines)

    1995-08-21

    Cophil Exploration Corp., a Filipino public company, spearheaded 1995 Philippine oil and gas exploration activity with the start of its gas delineation drilling operations in Libertad, northern Cebu. Cophil and its Australian partners, Coplex Resources NL and PacRim Energy NL, have set out to complete a seven well onshore drilling program within this block this year. The companies are testing two modest shallow gas plays, Libertad and Dalingding, and a small oil play, Maya, all in northern Cebu about 500 km southeast of Manila. Following a short discussion on the geology and exploration history of the Visayan basin, this article briefly summarizes Cophil`s ongoing Cebu onshore drilling program. Afterwards, discussion focuses on identified exploration opportunities in the basin`s offshore sector.

  18. Status of radiation processing in the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    Dela Rosa, A.M. [Philippine Nuclear Research Institute, Diliman, Quezon City (Philippines)

    2001-03-01

    Radiation processing, one of the well established applications of radiation and radioisotopes, has been successfully integrated into the industrial development of many countries worldwide. Environmental considerations embodied in the Montreal Protocol, the need for energy conservation brought about by escalating costs of fossil fuels, and the bright prospect of utilizing indigenous materials for value-added products have catalyzed the rapid development of radiation processing in the Asia region. This paper presents an overview of current developments in research activities and commercial applications of radiation processing in the Philippines. The areas of application include radiation sterilization of medical products, food irradiation, radiation vulcanization of natural rubber latex, radiation modification of the marine polysaccharide, carrageenan, and upgrading of cellulosic agriwaste by irradiation. (author)

  19. Factors Affecting Fertility Desires in the Philippines

    Directory of Open Access Journals (Sweden)

    Clarissa C. David

    2014-12-01

    Full Text Available Factors affecting fertility desires in the Philippines were examined using data from a national survey and from individual and group qualitative interviews involving 143 respondents. Fertility goals usually range from two to three children, but evidence suggests that they are dynamic and may change over a person’s lifetime. Qualitative interviews reveal that when negotiating about family size, it is the partner who wants more children that will be followed. A strong demand for gender balance among offspring creates a willingness to have more children than originally desired. Fertility goals increase over time among women. While those who start childbirth at a very young age successfully space their children, they tend to want larger families than those who start late. Initial fertility goals among women are generally low but may increase because of higher fertility desires among men, a demand for gender balance in children, and the desire for babies once their children have grown.

  20. Rapid diagnosis of vibriosis and white spot syndrome (WSS) in the culture of shrimp, Penaeus monodon in Philippines.

    Science.gov (United States)

    Caipang, Christopher Marlowe A; Aguana, Mary Paz N

    2010-10-01

    Viral and bacterial pathogens have raised serious concerns in the sustainability of the shrimp culture industry in the Philippines. Heavy mortality associated with luminous vibriosis and white spot syndrome virus (WSSV) infection has been the major problem besetting the industry. Using published PCR protocols for the diagnosis of vibriosis and white spot syndrome virus (WSSV) disease in shrimp, we optimized these assays that could be suited to the shrimp aquaculture setting in the Philippines. Genomic DNAs of Vibrio spp. that exhibited luminescence as well as those that grew on thiosulfate citrate bile salt sucrose agar (TCBS) were used for the PCR amplification of the ribonuclease P (RNase P) gene. There was differential amplification of the RNase P gene based on the phenotypic characters of the Vibrio spp. Similar results were also obtained using direct colony PCR of the bacterial colonies. White spot syndrome virus was also detected in the infected shrimp and there were differences in the detection frequency in relation to the tissues used for PCR amplification. Duplex PCR was also optimized that could be used for simultaneous detection of these pathogens in shrimp.

  1. Seasonal Genetic Changes of Aedes aegypti (Diptera: Culicidae) Populations in Selected Sites of Cebu City, Philippines.

    Science.gov (United States)

    Sayson, S L; Gloria-Soria, A; Powell, J R; Edillo, F E

    2015-07-01

    Aedes aegypti (L.) is the primary vector of dengue virus in the Philippines, where dengue is endemic. We examined the genetic changes of Ae. aegypti collected from three selected sites in Cebu city, Philippines, during the relatively wet (2011-2012) and dry seasons (2012 and 2013). A total of 493 Ae. aegypti adults, reared in the laboratory from field-collected larvae, were analyzed using 11 microsatellite loci. Seasonal variation was observed in allele frequencies and allelic richness. Average genetic differentiation (DEST=0.018; FST=0.029) in both dry seasons was higher, due to reduced Ne, than in the wet season (DEST=0.006; FST=0.009). Thus, average gene flow was higher in the wet season than in the dry seasons. However, the overall FST estimate (0.02) inclusive of the two seasons showed little genetic differentiation as supported by Bayesian clustering analysis. Results suggest that during the dry season the intense selection that causes a dramatic reduction of population size favors heterozygotes, leading to small pockets of mosquitoes (refuges) that exhibit random genetic differentiation. During the wet season, the genetic composition of the population is reconstituted by the expansion of the refuges that survived the preceding dry season. Source reduction of mosquitoes during the nonepidemic dry season is thus recommended to prevent dengue re-emergence in the subsequent wet season.

  2. Natural incidence of tomato viruses in the North of Iran

    Directory of Open Access Journals (Sweden)

    Alireza Mohammadi HAJIABADI

    2012-09-01

    Full Text Available A survey was conducted in Qazvin province in the North of Iran, to determine the incidence of tomato viruses including: Tobacco mosaic virus (TMV, Tomato yellow leaf curl virus (TYLCV, Tomato chlorotic spot virus (TCSV, Tomato bushy stunt virus (TBSV, Tomato spotted wilt virus (TSWV, Tomato ring spot virus (ToRSV, Tomato aspermy virus (TAV, Potato virus Y (PVY, Beet curly top virus (BCTV, and Cucumber mosaic virus (CMV. A total of 742 tomato symptomatic samples were collected during the summer of 2007 in five regions of Qazvin province (Qazvin, Takestan, Boeen-Zahra, Alborz and Abiyek and tested by enzyme-linked immunosorbent assay (ELISA. TSWV was detected in Alborz (4.4 % and Abiyek (3.57% regions but TMV and CMV were detected in all five regions. The greatest and least incidence of tomato viruses were recorded in Alborz (40.7 % and Takestan (11.1 %, respectively. The presence of these viruses was also evaluated in the weed hosts as natural sources of plant viruses. The greatest and least incidence of tomato viruses in weed hosts were recorded in Boeen-Zahra (25.6 % and Qazvin (12.8 %, respectively. TSWV was not detected in weeds. Transmission tests demonstrated that Thrips tabaci acts as TSWV carrier and Myzus persicae and Aphis gossypii were CMV carriers. Seed transmission tests were positive for TMV (13 tomato seedlings from 100 seedlings, but no TSWV transmission was observed through the seeds of infected tomato fruits.

  3. The Philippines, the East Asian 'developmental states' and education: a comparative analysis of why the Philippines failed to develop

    OpenAIRE

    Maca, M.; Morris, P.

    2012-01-01

    After WWII, the economic prospects of the Philippines, then the second-largest economy in Asia, were viewed positively, but by the mid-1970s it had become Asia?s developmental puzzle for its failure to sustain economic growth. In contrast during the same period, regional neighbours, Hong Kong, Taiwan, South Korea and Singapore, achieved previously unknown levels of economic growth and were dubbed the ?East Asian Tigers?. This article analyses the post-war development of the Philippines, focus...

  4. Smallholder rubber agroforestry system in Mindanao, Philippines: A village approach to climate change mitigation

    OpenAIRE

    Mercado, Agustin R., Jr.; Edralin, Don Immanuel; Arcinal, Gil A.; Reyes, Manuel R.

    2014-01-01

    This poster presented the impact, sustainability and adaptability of Rubber Agroforestry System (RAS) to smallholder farmers Claveria, Philippines. LTRA-12 (Conservation agriculture for food security in Cambodia and the Philippines)

  5. 78 FR 41421 - Certain Oil Country Tubular Goods From India, Korea, Philippines, Saudi Arabia, Taiwan, Thailand...

    Science.gov (United States)

    2013-07-10

    ..., Philippines, Saudi Arabia, Taiwan, Thailand, Turkey, Ukraine, and Vietnam of certain oil country tubular goods... COMMISSION Certain Oil Country Tubular Goods From India, Korea, Philippines, Saudi Arabia, Taiwan, Thailand, Turkey, Ukraine, and Vietnam; Institution of Antidumping and Countervailing Duty Investigations...

  6. Making contract farming work? : society and technology in Philippine transnational agribusiness

    NARCIS (Netherlands)

    Vellema, S.

    2002-01-01

    Keywords: contract farming, agribusiness, Philippines, Southeast Asia, asparagus, hybrid maizeContract farming is a widespread and important tool for organising agricultural production in line with corporate strategies and market demands. This book analyses how Philippine farmers and transnational a

  7. BG J. Franklin Bell and the Practice of Operational Art in the Philippines, 1901-1902

    Science.gov (United States)

    2011-12-01

    37 Daniel B. Schirmer and Stephen R. Shalom, eds., The Philippines Reader: a History of Colonialism, Neocolonialism , Dictatorship, and Resistance...Shalom, ed. The Philippines Reader: a History of Colonialism, Neocolonialism , Dictatorship, and Resistance. Boston: Souht End Press, 1999

  8. Preface to the Special Issue on "Geodynamics of Taiwan and the Philippines"

    Directory of Open Access Journals (Sweden)

    Win-Bin Cheng and Shou-Yeh Gong

    2015-01-01

    Full Text Available This special issue contains a collection of six papers dealing with the geology, geophysics, and tectonics of Taiwan and the Philippines. Most of these papers were presented at the fourth _ Earth Sciences International _ in Manila, Philippines, 8 - 9 June 2013. The tectonics of Taiwan and the Philippines are controlled mainly by three plates: the Indian, Eurasian, and Philippine Sea Plates. Taiwan and the Philippines are located next to each other in terms of geography and also plate tectonics. Luzon Island originated from the interactions between the South China Sea and Philippine Sea Plates. Taiwan was also created by collisions between the Philippine Sea Plate and Eurasian Plates. The convergence of these plates also results in many earthquakes and volcanic activities in the region. Both Taiwan and the Philippines face similar geological hazards, such as earthquakes, landslides, volcanic eruptions, and tsunamis.

  9. Making contract farming work? : society and technology in Philippine transnational agribusiness

    NARCIS (Netherlands)

    Vellema, S.

    2002-01-01

    Keywords: contract farming, agribusiness, Philippines, Southeast Asia, asparagus, hybrid maize

    Contract farming is a widespread and important tool for organising agricultural production in line with corporate strategies and market demands. This book analyses how Philippine farmers

  10. Molecular detection and characterization of sapovirus in hospitalized children with acute gastroenteritis in the Philippines.

    Science.gov (United States)

    Liu, Xiaofang; Yamamoto, Dai; Saito, Mariko; Imagawa, Toshifumi; Ablola, Adrianne; Tandoc, Amado O; Segubre-Mercado, Edelwisa; Lupisan, Socorro P; Okamoto, Michiko; Furuse, Yuki; Saito, Mayuko; Oshitani, Hitoshi

    2015-07-01

    Human sapovirus (SaV) is a causative agent of acute gastroenteritis. Recently, SaV detection has been increasing worldwide due to the emerging SaV genotype I.2. However, SaV infection has not been reported in the Philippines. To evaluate the prevalence and genetic diversity of SaV in hospitalized children aged less than 5 years with acute gastroenteritis. Stool samples were collected from children with acute gastroenteritis at three hospitals in the Philippines from June 2012 to August 2013. SaV was detected by reverse transcription real-time PCR, and the polymerase and capsid gene sequences were analyzed. Full genome sequencing and recombination analysis were performed on possible recombinant viruses. SaV was detected in 7.0% of the tested stool samples (29/417). In 10 SaV-positive cases, other viruses were also detected, including rotavirus (n=6), norovirus (n=2), and human astrovirus (n=2). Four known SaV genotypes (GI.1 [7], GI.2 [2], GII.1 [12], and GV [2]) and one novel recombinant (n=3) were identified by polymerase and capsid gene sequence analysis. Full genome sequencing revealed that the 5' nontranslated region (NTR) and nonstructural protein region of the novel recombinant were closely related to the GII.1 Bristol/98/UK variant, whereas the structural protein region and 3' NTR were closely related to the GII.4 Kumamoto6/Mar2003/JPN variant. SaV was regularly detected in hospitalized children due to acute gastroenteritis during the study period. A novel recombinant, SaV GII.1/GII.4, was identified in three cases at two different study sites. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  11. Field trial with oral vaccination of dogs against rabies in the Philippines

    Directory of Open Access Journals (Sweden)

    De Leon Renato

    2001-11-01

    Full Text Available Abstract Background The potential role of oral vaccination of dogs against rabies in the Philippines was investigated in terms of safety and efficacy. Methods Prior to the vaccination campaign, a house-to-house survey was carried out to collect data on the dog population in the study area, the coastal village of Mindoro. During the vaccination campaign all households were visited again, and all dogs encountered (>2 months old were, if possible, vaccinated. Furthermore, 14 dogs vaccinated were bled on different occasions. Results During the survey, a total of 216 dogs were counted, and none of these animals had previously been vaccinated against rabies. Only 17 dogs could be restrained and subsequently vaccinated directly by the vaccinators. Another 126 dogs were offered a local-made boiled intestine bait, containing a capsule filled with 3.0 ml SAD B19 (107.9 FFU/ml. The bait acceptance rate of dogs offered a bait was 96.1%. The vaccination coverage of the dog population (> 2 months old estimated by the number of animals vaccinated directly and the number of dogs that accepted a bait and subsequently punctured the vaccine container was 76%. Fifteen and 29 days after the vaccination campaign 6 and 10 dogs (n = 14 had rabies virus neutralizing antibody titres of ≥ 0.5 IU/ml, respectively. No unintentional contacts of nontarget species, including humans, with the vaccine virus were reported. Conclusions The results of the campaign show that oral vaccination of dogs against rabies is a promising supplementary method in dog rabies control in the Philippines.

  12. Numerical study on the curling and warping of hardened rigid pavement slabs

    Science.gov (United States)

    Qin, Yinghong

    In-service hardened concrete pavement suffers from environmental loadings caused by curling and warping of the slab. Traditionally, these loadings are computed on the basis of treating the slab as an elastic material, and of evaluating separately the curling and warping components. This dissertation simulates temperature distribution and moisture distribution through the slabs by use of a developed numerical model that couples the heat transfer and moisture transport. The computation of environmental loadings treats the slab as an elastic-viscous material, which considers the relaxation behavior and Pickett effect of the concrete. The heat transfer model considers the impacts of solar radiation, wind speed, air temperature, pavement slab albedo, etc. on the pavement temperature distribution. This dissertation assesses the difference between documented models that aim to predict pavement temperature, highlighting their pros and cons. The moisture transport model is unique for the documented models; it mimics the wetting and drying events occurring at the slab surface. These events are estimated by a proposed statistical algorithm, which is verified by field rainfall data. Analysis of the predicted results examines on the roles of the local air RH (relative humidity), wind speed, rainy pattern in the moisture distribution through the slab. The findings reveal that seasonal air RH plays a decisive role on the slab's moisture distribution; but wind speed and its daily variation, daily RH variation, and seasonal rainfall pattern plays only a secondary role. This dissertation sheds light on the computation of environmental loadings that in-service pavement slabs suffer from. Analysis of the computed stresses centers on the stress relaxation near the surface, stress evolution after the curing ends, and the impact of construction season on the stress's magnitude. An unexpected finding is that the total environmental loadings at the cyclically-stable state divert from the

  13. High order gradient, curl and divergence conforming spaces, with an application to NURBS-based IsoGeometric Analysis

    CERN Document Server

    Hiemstra, R R; Gerritsma, M I

    2012-01-01

    Conservation laws, in for example, electromagnetism, solid and fluid mechanics, allow an exact discrete representation in terms of line, surface and volume integrals. We develop high order interpolants, from any basis that is a partition of unity, that satisfy these integral relations exactly, at cell level. The resulting gradient, curl and divergence conforming spaces have the property that the conservation laws become completely independent of the basis functions. This means that the conservation laws are exactly satisfied even on curved meshes. As an example, we develop high order gradient, curl and divergence conforming spaces from NURBS - non uniform rational B-splines - and thereby generalize the compatible spaces of B-splines developed by Buffa et al.[1]. We give several examples of 2D Stokes flow calculations which result, amongst others, in a point wise divergence free velocity field.

  14. Guidelines and policies on collection of biological specimens in the Philippines. Philippine Congress, International Convention on Biodiversity.

    Science.gov (United States)

    Madulid, D A

    1996-04-01

    In October, 1993, 16 months after the United Nations approved the International Convention on Biodiversity held in Rio de Janeiro, June, 1992, the Philippine Congress ratified and adopted the Convention. This is a manifestation of the full support of the Philippines for the principles and policies adopted by the UN body on the conservation of biodiversity, sustainable development of biological resources and equitable sharing of benefits between users and owners of biodiversity resources. The Philippine scientific community has long recognized the need for and importance of a national guideline and policy with regard to the collection of plants and animals in the Philippines for scientific or commercial purposes. A series of consultative meetings were held by representatives of government agencies, non-government organizations, private organizations, academic and private persons concerned with biodiversity conservation to formulate national guidelines that regulate the collection of plant and animal specimens in the country. Guidelines were unanimously adopted by various government agencies and academia and a Memorandum of Agreement (MOA) was signed on September 28, 1990. Very recently a new document was drafted, specifically to serve as a guideline for those who desire to undertake sample collecting in the Philippines for biodiversity prospecting. The document is now being reviewed by government departments and agencies and will be presented to the President of the Philippines for signing as an Executive Order (EO). Once signed, this EO will serve as a national policy for bioprospecting in the country. The Philippines is one of the countries in Southeast Asia that has endorsed the adoption of regional guidelines on the collection of plant and animal organisms for drug development. The ASEAN Agreement on the Conservation of Nature and Natural Resources (1985). The Manila Declaration (1992) and lately, the Melaka Accord (1994), all of which were signed by various

  15. 38 CFR 17.350 - The program of assistance to the Philippines.

    Science.gov (United States)

    2010-07-01

    ... Republic of the Philippines and to furnishing medical services under 38 U.S.C. 1724 and 1732, and 38 CFR 17... to the Philippines. 17.350 Section 17.350 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS MEDICAL Grants to the Republic of the Philippines § 17.350 The program of assistance...

  16. A Bibliography of Philippine Language Dictionaries and Vocabularies. Special Monograph Issue, Number 30.

    Science.gov (United States)

    Hendrickson, Gail R., Comp.; Newell, Leonard E., Comp.

    This bibliography is a comprehensive listing of dictionaries and vocabularies, published and unpublished, of the Philippine languages. Introductory sections chronicle briefly the histories of Philippine lexicography and Philippine bibliographies, describe the scope of the present work, and outline the organization of the bibliography itself and…

  17. 77 FR 39735 - Stainless Steel Butt-Weld Pipe Fittings From Italy, Malaysia, and the Philippines

    Science.gov (United States)

    2012-07-05

    ...)] Stainless Steel Butt-Weld Pipe Fittings From Italy, Malaysia, and the Philippines Determination On the basis..., Malaysia, and the Philippines would be likely to lead to continuation or recurrence of material injury to... Pipe Fittings from Italy, Malaysia, and the Philippines: Inv. Nos. 731-TA-865-867 (Second...

  18. The Politics of “Public Opinion” in the Philippines

    Directory of Open Access Journals (Sweden)

    Eva-Lotta E. Hedman

    2010-01-01

    Full Text Available In May 2010, national elections in the Philippines saw front-runner presidential candidate Benigno “Noynoy” Aquino III win a landslide victory which set the stage for an orderly transition of power from the administration of Gloria Macapagal-Arroyo. This article argues that Aquino’s victory, rather than signalling a clear departure from the old ways of doing politics or the mere reproduction of established patterns of oligarchical politics, points towards a more gradual and limited change in the mobilisation of voters in the Philippines. This change, it is further argued, reflects in part the rise of “public opinion” as a social fact in Philippine politics and society in the period since the resurrection of formal democratic institutions and regular elections. The article identifies the broad parameters of the rise in polls and surveys in the Philippines, and, drawing on the critical insights of Pierre Bourdieu, examines the nature and significance of “public opinion” itself. However, the argument advanced here is a cautionary one, indicating that, while the emergence of public opinion as a social fact alters political calculations and dynamics associated with voter mobilisation, the politics of public opinion may only have limited transformative potential for democracy in the Philippines.

  19. Large-Deformation Curling Actuators Based on Carbon Nanotube Composite: Advanced-Structure Design and Biomimetic Application.

    Science.gov (United States)

    Chen, Luzhuo; Weng, Mingcen; Zhou, Zhiwei; Zhou, Yi; Zhang, Lingling; Li, Jiaxin; Huang, Zhigao; Zhang, Wei; Liu, Changhong; Fan, Shoushan

    2015-12-22

    In recent years, electroactive polymers have been developed as actuator materials. As an important branch of electroactive polymers, electrothermal actuators (ETAs) demonstrate potential applications in the fields of artificial muscles, biomimetic devices, robotics, and so on. Large-shape deformation, low-voltage-driven actuation, and ultrafast fabrication are critical to the development of ETA. However, a simultaneous optimization of all of these advantages has not been realized yet. Practical biomimetic applications are also rare. In this work, we introduce an ultrafast approach to fabricate a curling actuator based on a newly designed carbon nanotube and polymer composite, which completely realizes all of the above required advantages. The actuator shows an ultralarge curling actuation with a curvature greater than 1.0 cm(-1) and bending angle larger than 360°, even curling into a tubular structure. The driving voltage is down to a low voltage of 5 V. The remarkable actuation is attributed not only to the mismatch in the coefficients of thermal expansion but also to the mechanical property changes of materials during temperature change. We also construct an S-shape actuator to show the possibility of building advanced-structure actuators. A weightlifting walking robot is further designed that exhibits a fast-moving motion while lifting a sample heavier than itself, demonstrating promising biomimetic applications.

  20. Tourism and Crime: Evidence from the Philippines

    Directory of Open Access Journals (Sweden)

    Rosalina Palanca-Tan

    2015-12-01

    Full Text Available Using panel data gathered from 16 regions of the Philippines for the period 2009–11, this paper investigates the relationship between tourism and crime. The findings of the study show that the relation between tourism and crime may largely depend on the characteristics of visitors and the types of crime. For all types of crime and their aggregate, no significant correlation between the crime rate (defined as the number of crime cases divided by population and total tourist arrivals is found. However, a statistically significant positive relation is found between foreign tourism and robbery and theft cases as well as between overseas Filipino tourism and robbery. On the other hand, domestic tourism is not significantly correlated with any of the four types of crimes. These results, together with a strong evidence of the negative relationship between crime and the crime clearance efficiency, present much opportunity for policy intervention in order to minimize the crime externality of the country’s tourism-led development strategy.

  1. The skin whitening industry in the Philippines.

    Science.gov (United States)

    Mendoza, Roger Lee

    2014-05-01

    Skin whitening is a big and booming industry in many developing countries. Its popularity owes mainly to post-colonial, internalized racism. This study examines whether government intervention is necessary and more efficient than market-driven approaches in addressing the health risks and harms associated with skin whitening. We gathered empirical data on the quality and quantity of health-related information about skin whiteners with a multi-stage probability sample of consumers in two cities in the Philippines (n=110; α=0.05). Regardless of their socio-demographic characteristics, we find that cognitive biases and information asymmetries build and sustain consumers' trust in manufacturers and distributors of skin whiteners while, paradoxically, breeding uncertainties over the integrity of these products. The results are product adulteration and misbranding, leading to pricing advantages for toxic whiteners over safer products. This has impeded regulatory efficacy. We recommend anchoring government intervention in transaction cost-reduction, containing the externalities of skin bleaching, and institutionalizing third party partnerships. Failure to do so would leave consumers extremely vulnerable to the forces of supply and demand that favor toxic whiteners, particularly in a market where voluntary collective action is difficult to organize.

  2. Molecular characterization and experimental host range of an isolate of Wissadula golden mosaic St. Thomas virus.

    Science.gov (United States)

    Collins, A M; Mujaddad-ur-Rehman, Malik; Brown, J K; Reddy, C; Wang, A; Fondong, V; Roye, M E

    2009-12-01

    Partial genome segments of a begomovirus were previously amplified from Wissadula amplissima exhibiting yellow-mosaic and leaf-curl symptoms in the parish of St. Thomas, Jamaica and this isolate assigned to a tentative begomovirus species, Wissadula golden mosaic St. Thomas virus. To clone the complete genome of this isolate of Wissadula golden mosaic St. Thomas virus, abutting primers were designed to PCR amplify its full-length DNA-A and DNA-B components. Sequence analysis of the complete begomovirus genome obtained, confirmed that it belongs to a distinct begomovirus species and this isolate was named Wissadula golden mosaic St. Thomas virus-[Jamaica:Albion:2005] (WGMSTV-[JM:Alb:05]). The genome of WGMSTV-[JM:Alb:05] is organized similar to that of other bipartite Western Hemisphere begomoviruses. Phylogenetic analyses placed the genome components of WGMSTV-[JM:Alb:05] in the Abutilon mosaic virus clade and showed that the DNA-A component is most closely related to four begomovirus species from Cuba, Tobacco leaf curl Cuba virus, Tobacco leaf rugose virus, Tobacco mottle leaf curl virus, and Tomato yellow distortion leaf virus. The putative Rep-binding-site motif in the common region of WGMSTV-[JM:Alb:05] was observed to be identical to that of Chino del tomate virus-Tomato [Mexico:Sinaloa:1983], Sida yellow mosaic Yucatan virus-[Mexico:Yucatan:2005], and Tomato leaf curl Sinaloa virus-[Nicaragua:Santa Lucia], suggesting that WGMSTV-[JM:Alb:05] is capable of forming viable pseudo-recombinants with these begomoviruses, but not with other members of the Abutilon mosaic virus clade. Biolistic inoculation of test plant species with partial dimers of the WGMSTV-[JM:Alb:05] DNA-A and DNA-B components showed that the virus was infectious to Nicotiana benthamiana and W. amplissima and the cultivated species Phaseolus vulgaris (kidney bean) and Lycopersicon esculentum (tomato). Infected W. amplissima plants developed symptoms similar to symptoms observed under field

  3. Ebola Virus and Marburg Virus

    Science.gov (United States)

    Diseases and Conditions Ebola virus and Marburg virus By Mayo Clinic Staff Ebola virus and Marburg virus are related viruses that cause hemorrhagic ... Africa, where sporadic outbreaks have occurred for decades. Ebola virus and Marburg virus live in animal hosts, ...

  4. Ebola Virus and Marburg Virus

    Science.gov (United States)

    Ebola virus and Marburg virus Overview By Mayo Clinic Staff Ebola virus and Marburg virus are related viruses that ... Africa, where sporadic outbreaks have occurred for decades. Ebola virus and Marburg virus live in animal hosts, ...

  5. Curling probe measurement of large-volume pulsed plasma confined by surface magnetic field

    Science.gov (United States)

    Pandey, Anil; Sakakibara, Wataru; Matsuoka, Hiroyuki; Nakamura, Keiji; Sugai, Hideo; Chubu University Team; DOWA Thermotech Collaboration

    2015-09-01

    Curling probe (CP) has recently been developed which enables the local electron density measurement even in plasma for non-conducting film CVD. The electron density is obtained from a shift of resonance frequency of spiral antenna in discharge ON and OFF monitored by a network analyzer (NWA). In case of a pulsed glow discharge, synchronization of discharge pulse with frequency sweep of NWA must be established. In this paper, we report time and space-resolved CP measurement of electron density in a large volume plasma (80 cm diameter, 110 cm length) confined by surface magnetic field (multipole cusp field ~0.03 T). For plasma-aided modification of metal surface, the plasma is produced by 1 kV glow discharge at pulse frequency of 0.3 - 25 kHz with various duty ratio in gas (Ar, N2, C2H2) at pressure ~ 1 Pa. A radially movable CP revealed a remarkable effect of surface magnetic confinement: detach of plasma from the vessel wall and a fairly uniform plasma in the central region. In afterglow phase, the electron density was observed to decrease much faster in C2H2 discharge than in Ar discharge.

  6. Curling probe measurement of a large-volume pulsed plasma with surface magnetic confinement

    Science.gov (United States)

    Pandey, A.; Tashiro, H.; Sakakibara, W.; Nakamura, K.; Sugai, H.

    2016-12-01

    A curling probe (CP) based on microwave resonance is applied to the measurement of electron density in a pulsed DC glow discharge under surface magnetic confinement (SMC) provided by a number of permanent magnets on a chamber wall. Owing to the SMC effects, a 1 m scale large-volume plasma is generated by a relatively low voltage (~1 kV) at low pressure (~1 Pa) in various gases (Ar, CH4, and C2H2). Temporal variation of the electron density is measured for pulse frequency f  =  0.5-25 kHz for various discharge-on times (T ON) with a high resolution time (~0.2 µs), using the on-point mode. In general, the electron density starts to increase at time t  =  0 after turn-on of the discharge voltage, reaches peak density at t  =  T ON, and then decreases after turn-off. The peak electron density is observed to increase with the pulse frequency f for constant T ON owing to the residual plasma. This dependence is successfully formulated using a semi-empirical model. The spatio-temporal evolution of the cathode sheath in the pulsed discharge is revealed by a 1 m long movable CP. The measured thickness of the high-voltage cathode fall in a steady state coincides with the value of the so-called Child-Langmuir sheath.

  7. Curl flux induced drift in stochastic differential equations in the zero-mass limit

    Science.gov (United States)

    Wang, Jinhua; Yuan, Bo

    2016-11-01

    We consider the nonlinear stochastic dynamics of dissipative Hamiltonian systems with state-dependent friction and diffusion connected by the fluctuation-dissipation relation in high dimensions. The system under study has a close connection to Ao's framework in constructing a dynamical potential for non-equilibrium processes without detailed balance. We study the limiting case where the mass approaches zero and give a new and complete derivation of effective stochastic differential equations. Using the Ito stochastic integral convention, we show that the limiting effective Langevin equations have a new drift term. This extra term happens to be identical to the corresponding anti-Ito (or isothermal) integral (requiring constant temperature) in one dimension. We, however, cannot obtain this additional drift term using conventional stochastic integrals in high dimension. It is interesting to note that in a high-dimensional system, a curl flux induced drift may appear even if the diffusion matrix is constant. Our findings are supported by numerical simulations. We further analyze and discuss the role of this new drift term in calculating the classic escape time. For the first time, to our knowledge, the relation between the escape rate and the anti-Ito integral is presented. We also demonstrate that the derived diffusion equations give a new sampling algorithm which can increase convergence speed in a simple two-dimensional example.

  8. FUNCTIONAL AND NEUROMUSCULAR CHANGES IN THE HAMSTRINGS AFTER DROP JUMPS AND LEG CURLS

    Directory of Open Access Journals (Sweden)

    Nejc Sarabon

    2013-09-01

    Full Text Available The purpose of this study was to use a holistic approach to investigate changes in jumping performance, kinaesthesia, static balance, isometric strength and fast stepping on spot during a 5-day recovery period, following an acute bout of damaging exercise consisted of drop jumps and leg curls, where specific emphasis was given on the hamstring muscles. Eleven young healthy subjects completed a series of highly intensive damaging exercises for their hamstring muscles. Prior to the exercise, and during the 5-day recovery period, the subjects were tested for biochemical markers (creatine kinase, aspartate aminotransferase, and lactate dehydrogenase, perceived pain sensation, physical performance (squat jump, counter movement jump, maximal frequency leg stamping, maximal isometric torque production and maximally explosive isometric torque production, kinaesthesia (active torque tracking and static balance. We observed significant decreases in maximal isometric knee flexion torque production, the rate of torque production, and majority of the parameters for vertical jump performance. No alterations were found in kinaesthesia, static balance and fast stepping on spot. The highest drop in performance and increase in perceived pain sensation generally occurred 24 or 48 hours after the exercise. Damaging exercise substantially alters the neuromuscular functions of the hamstring muscles, which is specifically relevant for sports and rehabilitation experts, as the hamstrings are often stretched to significant lengths, in particular when the knee is extended and hip flexed. These findings are practically important for recovery after high-intensity trainings for hamstring muscles

  9. Timing of Surface-Rupturing Earthquakes on the Philippine Fault Zone in Central Luzon Island, Philippines

    Science.gov (United States)

    Tsutsumi, H.; Daligdig, J. A.; Goto, H.; Tungol, N. M.; Kondo, H.; Nakata, T.; Okuno, M.; Sugito, N.

    2006-12-01

    The Philippine fault zone is an arc-parallel left-lateral strike-slip fault zone related to oblique subduction of the Philippine Sea plate beneath the Philippine island arc. The fault zone extends for about 1300 km from the Luzon Island southward to the Mindanao Island. This fault zone has been seismically active with more than 10 earthquakes greater than M7 in the last century. The July 16, 1990, Luzon earthquake was the largest event that produced 120-km-long surface rupture along the Digdig fault. The coseismic displacement was predominantly left-lateral strike-slip with maximum slip of about 6 m. The Philippine fault zone in the Luzon Island consists of four left-stepping en echelon faults: the San Manuel, San Jose, Digdig, and Gabaldon faults from north to south. Historical documents and geomorphic data suggest that the San Manuel and Gabaldon faults ruptured most recently during historical earthquakes in 1796 and 1645, respectively. However, paleoseismic activities and slip rates for these faults were poorly constrained. In order to reconstruct chronology of surface-rupturing earthquakes, we excavated multiple trenches across these faults in the past three years. We have excavated two sites, San Gregorio and Puncan sites, across the Digdig fault. At the both sites, we identified near vertical fault zones that contain evidence for four surface-rupturing earthquakes during the past 2000 years, including the 1990 rupture. The timing of the penultimate earthquake is constrained to prior to 1400 AD, suggesting that the Digdig fault did not rupture during the 1645 earthquake. The average recurrence interval of the Digdig fault is about 600 years. A left-lateral slip rate of 8-13 mm/yr was obtained for the Digdig fault based on stream offsets and age of alluvial fan at San Juan in the central portion of the fault. For the San Jose fault, we excavated two trenches north of downtown San Jose. The sediments exposed on the trench walls were warped into a monocline by

  10. Fontes de resistência em tomateiro aos begomovírus bissegmentados Tomato yellow spot virus e Tomato severe rugose virus Sources of resistance in tomato to bipartite begomoviruses Tomato yellow spot virus and Tomato severe rugose virus

    OpenAIRE

    2012-01-01

    Os begomovírus causam doenças de grande importância econômica em diversas culturas, principalmente em regiões tropicais e subtropicais. Juntamente com outras famílias de vírus, os begomovírus têm causado grande prejuízo para os produtores de tomate in natura e para processamento industrial. O objetivo deste trabalho foi avaliar o comportamento de 11 genótipos resistentes ao Tomato yellow leaf curl virus (TYLCV) frente à infecção pelos begomovírus Tomato yellow spot virus (ToYSV) e Tomato seve...

  11. Modeling Philippine Stock Exchange Composite Index Using Time Series Analysis

    Science.gov (United States)

    Gayo, W. S.; Urrutia, J. D.; Temple, J. M. F.; Sandoval, J. R. D.; Sanglay, J. E. A.

    2015-06-01

    This study was conducted to develop a time series model of the Philippine Stock Exchange Composite Index and its volatility using the finite mixture of ARIMA model with conditional variance equations such as ARCH, GARCH, EG ARCH, TARCH and PARCH models. Also, the study aimed to find out the reason behind the behaviorof PSEi, that is, which of the economic variables - Consumer Price Index, crude oil price, foreign exchange rate, gold price, interest rate, money supply, price-earnings ratio, Producers’ Price Index and terms of trade - can be used in projecting future values of PSEi and this was examined using Granger Causality Test. The findings showed that the best time series model for Philippine Stock Exchange Composite index is ARIMA(1,1,5) - ARCH(1). Also, Consumer Price Index, crude oil price and foreign exchange rate are factors concluded to Granger cause Philippine Stock Exchange Composite Index.

  12. Curl flux, coherence, and population landscape of molecular systems: Nonequilibrium quantum steady state, energy (charge) transport, and thermodynamics

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Z. D. [Department of Physics and Astronomy, SUNY Stony Brook, New York 11794 (United States); Wang, J. [Department of Physics and Astronomy, SUNY Stony Brook, New York 11794 (United States); Department of Chemistry, SUNY Stony Brook, New York 11794 (United States); State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022 (China)

    2014-06-28

    We established a theoretical framework in terms of the curl flux, population landscape, and coherence for non-equilibrium quantum systems at steady state, through exploring the energy and charge transport in molecular processes. The curl quantum flux plays the key role in determining transport properties and the system reaches equilibrium when flux vanishes. The novel curl quantum flux reflects the degree of non-equilibriumness and the time-irreversibility. We found an analytical expression for the quantum flux and its relationship to the environmental pumping (non-equilibriumness quantified by the voltage away from the equilibrium) and the quantum tunneling. Furthermore, we investigated another quantum signature, the coherence, quantitatively measured by the non-zero off diagonal element of the density matrix. Populations of states give the probabilities of individual states and therefore quantify the population landscape. Both curl flux and coherence depend on steady state population landscape. Besides the environment-assistance which can give dramatic enhancement of coherence and quantum flux with high voltage at a fixed tunneling strength, the quantum flux is promoted by the coherence in the regime of small tunneling while reduced by the coherence in the regime of large tunneling, due to the non-monotonic relationship between the coherence and tunneling. This is in contrast to the previously found linear relationship. For the systems coupled to bosonic (photonic and phononic) reservoirs the flux is significantly promoted at large voltage while for fermionic (electronic) reservoirs the flux reaches a saturation after a significant enhancement at large voltage due to the Pauli exclusion principle. In view of the system as a quantum heat engine, we studied the non-equilibrium thermodynamics and established the analytical connections of curl quantum flux to the transport quantities such as energy (charge) transfer efficiency, chemical reaction efficiency, energy

  13. A synoptic review of the ant genera (Hymenoptera, Formicidae) of the Philippines

    Science.gov (United States)

    General, David M.; Alpert, Gary D.

    2012-01-01

    Abstract An overview of the history of myrmecology in the Philippine archipelago is presented. Keys are provided to the 11 ant subfamilies and the 92 ant genera known from the Philippines. Eleven ant genera (12%), including 3 undescribed genera, are recorded for the first time from the Philippines. The biology and ecology of the 92 genera, illustrated by full-face and profile photo-images, of Philippine ants are summarized in the form of brief generic accounts. A bibliography of significant taxonomic and behavioral papers on Philippine ants and a checklist of valid species and subspecies and their island distributions are provided. PMID:22767999

  14. A synoptic review of the ant genera (Hymenoptera, Formicidae of the Philippines

    Directory of Open Access Journals (Sweden)

    David General

    2012-06-01

    Full Text Available An overview of the history of myrmecology in the Philippine archipelago is presented. Keys are provided to the 11 ant subfamilies and the 92 ant genera known from the Philippines. Eleven ant genera (12%, including 3 undescribed genera, are recorded for the first time from the Philippines. The biology and ecology of the 92 genera, illustrated by full-face and profile photo-images, of Philippine ants are summarized in the form of brief generic accounts. A bibliography of significant taxonomic and behavioral papers on Philippine ants and a checklist of valid species and subspecies and their island distributions are provided.

  15. Todaro migration and primacy models: relevance to the urbanization of the Philippines.

    Science.gov (United States)

    Cuervo, J C; Kim Hin, D H

    1998-08-01

    "This paper looks into the set of factors that [influence] the urbanization of the Philippines, a fast-growing developing economy in South East Asia. The paper demonstrates that the ¿migration primacy urbanization model' is an appropriate one that is able to explain the urbanization case in the Philippines. The model draws supporting evidence from rank-size distribution analysis of major cities in the Philippines, a detailed examination of historical, geopolitical and economic forces which have evolved in the development of the Philippines as a sovereign state, and the applicability of the Todaro model on rural-urban migration to the Philippines." excerpt

  16. R & D Activities on Urban Forestry in the Philippines

    Institute of Scientific and Technical Information of China (English)

    LeilaC.America

    2004-01-01

    This paper highlights the research and development activities being done on urban forestry in the Philippines. The role that R&D plays in urban forestry is likewise discussed in the introduction. Recommended tree species for urban planting are presented including their characteristics and distribution. Researches related to urban forestry being conducted are listed with a short description of each project. Development activities on urban forestry are likewise discussed. Finally, recommended R&D agenda and researchable areas based on the Philippine Agenda 21 and the Department of Environment and Natural Resources (DENR) R&D Framework are presented.

  17. The feminization of migration from a Philippine perspective

    Directory of Open Access Journals (Sweden)

    Natalia Ribas Mateos

    2005-01-01

    Full Text Available This paper deals with the tendency towards the increase in female migration and uses as a paradigm the example of the migration of Philippine females to Spain. The starting point is the connection between the processes associated with globalization and other numerous changes which have led to the exodus of Philippine women to work as domestic servants in the large cities of the south of Europe. Finally, the various issues which have been used to illustrate this paper converge in an overall vision of this specific situation which is considered as an “icon of domesticity”.

  18. The Rockefeller agenda for American/Philippines nursing relations.

    Science.gov (United States)

    Brush, B L

    1995-10-01

    The Philippines leads all countries in global nurse emigration. Today, Filipino nurses represent over 75% of the foreign nurse labor force recruited to and working in American hospitals, most of which are inner-city municipally operated institutions with reported shortages of nursing personnel. This article examines the historical roots of the American/Philippines nursing relationship more generally and the particular role of the Rockefeller Foundation in the 20th-century emigration patterns and work practices of Filipino nurses. Examination of one group of nurse workers enhances an understanding of the ways in which social, cultural, economic, and political factors influence broader health care decisions.

  19. The Philippine Bases: Continuing Utility in a Changing Strategic Context,

    Science.gov (United States)

    1980-02-01

    Mathews, "The Philippines’ Imelda Marcos Awaits the Call to Power," Washington Post, 22 September 1978, p. A19; and Kit G. Machado , "The Philippines 1978... Machado , "The Philip- pines 1978," pp. 137-139; and Sheilah Ocampo, "Marcos Still Calls the Shots," Far Eastern Economic Review, 10 August 1979, p... assis - tance-grants, loans and sales credits), the length of the agree- ment, and the renewal stipulations. Since the breakdown of Marcos-Ford

  20. Ageratum enation virus Infection Induces Programmed Cell Death and Alters Metabolite Biosynthesis in Papaver somniferum

    Directory of Open Access Journals (Sweden)

    Ashish Srivastava

    2017-07-01

    Full Text Available A previously unknown disease which causes severe vein thickening and inward leaf curl was observed in a number of opium poppy (Papaver somniferum L. plants. The sequence analysis of full-length viral genome and associated betasatellite reveals the occurrence of Ageratum enation virus (AEV and Ageratum leaf curl betasatellite (ALCB, respectively. Co-infiltration of cloned agroinfectious DNAs of AEV and ALCB induces the leaf curl and vein thickening symptoms as were observed naturally. Infectivity assay confirmed this complex as the cause of disease and also satisfied the Koch’s postulates. Comprehensive microscopic analysis of infiltrated plants reveals severe structural anomalies in leaf and stem tissues represented by unorganized cell architecture and vascular bundles. Moreover, the characteristic blebs and membranous vesicles formed due to the virus-induced disintegration of the plasma membrane and intracellular organelles were also present. An accelerated nuclear DNA fragmentation was observed by Comet assay and confirmed by TUNEL and Hoechst dye staining assays suggesting virus-induced programmed cell death. Virus-infection altered the biosynthesis of several important metabolites. The biosynthesis potential of morphine, thebaine, codeine, and papaverine alkaloids reduced significantly in infected plants except for noscapine whose biosynthesis was comparatively enhanced. The expression analysis of corresponding alkaloid pathway genes by real time-PCR corroborated well with the results of HPLC analysis for alkaloid perturbations. The changes in the metabolite and alkaloid contents affect the commercial value of the poppy plants.

  1. SPace Radar Image of Mt. Pinatubo, Philippines

    Science.gov (United States)

    1999-01-01

    This is a false color L-band and C-band image of the area around Mount Pinatubo in the Philippines, centered at about 15 degrees north latitude, 120.5 degrees east longitude. This image was acquired by the Spaceborne Imaging Radar-C and X-Band Synthetic Aperture Radar (SIR-C/X-SAR) aboard the space shuttle Endeavour on orbit 78 on April 13, 1994. The false-color composite is made by displaying the L-band HH return in red, the L-band HV return in green and the C-band HV return in blue. The area shown is approximately 45 by 68 kilometers (28 by 42 miles). The main volcanic crater on Mount Pinatubo produced by the June 1991 eruptions, and the steep slopes on the upper flanks of the volcano, are easily seen in this image. The red color on the high slopes show the rougher ash deposited during the 1991 eruption. The dark drainages are the smooth mudflows which continue to flood the river valleys after heavy rain. Radar images such as this one can be used to identify the areas flooded by mudflows, which are difficult to distinguish visually, and to assess the rate at which the erosion and deposition continues. A key aspect of the second SIR-C/X-SAR mission in August 1994 will be to collect a second image of Pinatubo during the summer monsoon season -- new mudflows will have occurred -- and to evaluate the short-term changes. The 1991 eruption of Mount Pinatubo in the Philippines is well known for its near-global effects on the atmosphere and climate due to the large amount of sulfur dioxide that was injected into the upper atmosphere. What is less widely known is that even today the volcano continues to be a major hazard to the people who have returned to the area around the volcano. Dangerous mudflows (called 'lahars') are often generated by heavy rains, and these can still sweep down river valleys and wash out roads and villages, or bury low lying areas in several meters of mud and volcanic debris. These mudflows will continue to be a severe hazard around Pinatubo for

  2. Expressing activity of promoter elements of large intergenic region from cotton leaf curl virus in host plant*

    Institute of Scientific and Technical Information of China (English)

    XIE; Yingqiu; (

    2001-01-01

    [1]Jiao Jiujiu, Grey hydrogeologic system analysis and time series model, Survey Science and Technology (in Chinese), 1987,(10): 39-43.[2]Li Shuwen, Wang Baolai, Xiao Guoqiang, A compound model of grey and periodic scrape and its application in groundwater prediction, Journal of Hebei Institute of Architectural Science & Technology (in Chinese), 1992, (3): 246-251.[3]Wang Qingyin, Li Shuwen, Grey distributed parameter model and groundwater analog, Journal of Hebei Institute of Architectural Science & Technology (in Chinese), 1992, (3): 66-70.[4]Guo Chunqing, Xia Riyuan, Liu Zhenglin, Gray Systematic Theory and Methodological Study of Krast Groundwater Resources Evaluation (in Chinese), Beijing: Geological Publishing House, 1993, 3-60.[5]Wang Qingyin, Liu Kaidi, The Mathematical Method of Grey Systematic Theory and Its Application (in Chinese), Chengdu: Publishing House of Southwestern China University of Communication, 1990, 23-27.[6]Wang Qingyin, Wu Heqing, The concept of grey number and its property, in Proceedings of NAFIPS98, USA, 1998,45-49.[7]Givoli, D., Doukhovni, I., Finite element programming approach for contact problems with geometrical nonlinearity, Computers and Structures, 1996, (8): 31-41.[8]Li Shuwen, Wang Zhiqiang, Wu Qiang, The superiority of storage-centered finite element method in solving seepage problem, Coal Geology and Exploration (in Chinese), 1999, (5): 46-49.

  3. Initial development of a set of introgression lines from Solanum peruvianum PI 126944 into tomato:exploitation of resistance to viruses

    OpenAIRE

    Julián Rodríguez, Olga; Herraiz García, Francisco Javier; Corella, S.; Lolli Rodríguez, María Iciar Di; Soler Aleixandre, Salvador; Díez Niclós, Mª José Teresa De Jesús; Pérez De Castro, Ana María

    2013-01-01

    Resistance to Tomato yellow leaf curl virus (TYLCV) and Tomato spotted wilt virus (TSWV), among other diseases, has been reported in Solanum peruvianum PI 126944. Introgression lines (ILs) from S. peruvianum PI 126944 into the genetic background of cultivated tomato (S. lycopersicum) are being developed. Several generations were derived from three interspecific hybrids previously obtained.A lot of crosses and embryo rescue were required until the third backcross, due to the high degree of ...

  4. A Brief History of Educational Assessment in the Philippines

    Science.gov (United States)

    Magno, Carlo

    2010-01-01

    The report presents a primer on the history of educational assessment in the Philippines. The history of educational assessment is described by the different pillars that contributed to its development. These factors include timelines of government mandates, studies done in the national level, universities that shape experts in the field,…

  5. Philippines: The Role of Language and Education in Globalization

    Science.gov (United States)

    Quijano, Consuelo A.

    2012-01-01

    International education has never been discussed in-depth by many citizens of the Philippines. International education refers to a study abroad program or an approach to prepare students to function in a global society. The focus of discussion is more on primary, secondary, and tertiary education. In any level of education, language plays a vital…

  6. Quo Vadis? LIS Postgraduate Education in the Philippines

    Science.gov (United States)

    Dizon, Fernan R.; Sagun, Karryl Kim A.; Alfiler-Macalalad, Ana Grace P.

    2011-01-01

    The paper intends to shed light on the predicament faced by many Filipino. Librarians: the lack of local institutions offering a library and information science (LIS) postgraduate degree. The paper aims to reveal the state of Philippine LIS postgraduate education by considering the number of librarians who have pursued and are still pursuing…

  7. Skills for the Labor Market in the Philippines

    Science.gov (United States)

    Di Gropello, Emanuela

    2010-01-01

    The Philippines has experienced overall growth over these last twenty years, but the growth of the manufacturing sector has been sluggish and the country has lost innovation capacity. Re-gaining momentum will depend on many factors, but skills have a key role to play to support the growing service sector, help improve the competitiveness of the…

  8. Biodistribution of the Informal Group Basommatophora in the Philippines

    Directory of Open Access Journals (Sweden)

    Patrick Noel Y. Young

    2014-06-01

    Full Text Available Basommatophora is an informal group within the molluscan subclass Pulmonata comprising of air-breathing freshwater snails that are typically characterized by eyespots located at the base of two noncontractile tentacles and two external genital orifices. They also have varied shell structures and habitats, not only within the group but also within families. Families of the Basommatophora are highly ubiquitous and may play a role in the life cycles of various parasites of humans and animals. Basommatophora has a worldwide geographical distribution across freshwater, terrestrial and marine habitats. However, little is known on their distribution in the Philippines. This report focuses on describing the biogeographical distribution of the basommatophorans in the Philippines through data gathered from museum collections, foreign databases accessed online, and identification of species found in various literatures. A qualitative description of the distribution of each Basommatophora family in the Philippines is given by distribution maps, indicating locations where specimens were collected and/or identified. A total of 336 counts of basommatophorans from 22 genera were encountered from available literature, museums and public databases. The majority of the occurrences are from the genera Siphonaria. The data and maps generated describe most of the distribution to be in Luzon, with Visayas and Mindanao having close counts with each other. The Philippines has the third most occurrences and genera of basommatophorans of all tropical countries in the world. However, the true diversity of the group could be higher if a more systematic sampling of the archipelago is conducted.

  9. Understanding Student Attitudes toward Bible Reading: A Philippine Experience

    Science.gov (United States)

    Baring, Rito V.

    2008-01-01

    Reflecting from the Philippine experience, this article explores an emerging picture that characterizes contemporary Bible reading attitudes of college students. Six new attitude factor definitions are developed following the development of the Bible Reading (BR) attitude scale for college students constructed by this author in a separate study.…

  10. Government officials' representation of nurses and migration in the Philippines.

    Science.gov (United States)

    Masselink, Leah E; Daniel Lee, Shoou-Yih

    2013-01-01

    During the past few decades, the nursing workforce has been in crisis in the United States and around the world. Many health care organizations in developed countries recruit nurses from other countries to maintain acceptable staffing levels. The Philippines is the centre of a large, mostly private nursing education sector and an important supplier of nurses worldwide, despite its weak domestic health system and uneven distribution of health workers. This situation suggests a dilemma faced by developing countries that train health professionals for overseas markets: how do government officials balance competing interests in overseas health professionals' remittances and the need for well-qualified health professional workforces in domestic health systems? This study uses case studies of two recent controversies in nursing education and migration to examine how Philippine government officials represent nurses when nurse migration is the subject of debate. The study finds that Philippine government officials cast nurses as global rather than domestic providers of health care, implicating them in development more as sources of remittance income than for their potential contributions to the country's health care system. This orientation is motivated not simply by the desire for remittance revenues, but also as a way to cope with overproduction and lack of domestic opportunities for nurses in the Philippines.

  11. Records & reputations: Everyday politics of a Philippine Development NGO

    NARCIS (Netherlands)

    Hilhorst, D.

    2000-01-01

    This study looks into the working of policies, practices and accountability of NGOs. It is based on fieldwork with one development NGO in the Cordillera of the Philippines: the Cordillera Women NGO, or CWNGO (a pseudonym). Through this study I wanted to find out why certain groups of actors form org

  12. Organ donation in the Philippines: should the dead do more?

    Science.gov (United States)

    de Castro, Leonardo D

    2014-01-01

    This paper asks whether the Philippines should focus on ways of dealing with end-stage renal disease by getting more transplantable kidneys from the dead. Would it be more ethical to put the burden to donate on the dead (who have already lost their chance to consent) than on the living (who can consent)? Given the risks involved in undergoing nephrectomy and the lack of benefits arising from the procedure to donors, the dead should be the first to put their kidneys on the line. In the Philippines, unfortunately, living donors have had to bear the greater burden in this regard. Starting with a brief account of developments surrounding the impact of the Declaration of Istanbul on the situation in the Philippines as well as in other countries, the paper examines what the living have been expected to do, what they have actually done, and what lessons the experience with living donors offers for the understanding of cadaver transplants. The paper then looks at possible ways of increasing the sources of kidneys for transplantation and asks if these ways could be implemented successfully and ethically in the Philippines.

  13. Mapping the Philippines' mangrove forests using Landsat imagery

    Science.gov (United States)

    Long, J.B.; Giri, C.

    2011-01-01

    Current, accurate, and reliable information on the areal extent and spatial distribution of mangrove forests in the Philippines is limited. Previous estimates of mangrove extent do not illustrate the spatial distribution for the entire country. This study, part of a global assessment of mangrove dynamics, mapped the spatial distribution and areal extent of the Philippines' mangroves circa 2000. We used publicly available Landsat data acquired primarily from the Global Land Survey to map the total extent and spatial distribution. ISODATA clustering, an unsupervised classification technique, was applied to 61 Landsat images. Statistical analysis indicates the total area of mangrove forest cover was approximately 256,185 hectares circa 2000 with overall classification accuracy of 96.6% and a kappa coefficient of 0.926. These results differ substantially from most recent estimates of mangrove area in the Philippines. The results of this study may assist the decision making processes for rehabilitation and conservation efforts that are currently needed to protect and restore the Philippines' degraded mangrove forests. ?? 2011 by the authors; licensee MDPI, Basel, Switzerland.

  14. Piper (Piperaceae) in the Philippine Islands: the climbing species

    NARCIS (Netherlands)

    Gardner, R.O.

    2006-01-01

    Piper in the Philippine Islands is reviewed. Fifteen climbing species are recognized (many fewer than in previous treatments) and distinguished in a key. Most are widely distributed through Malesia, with ranges that end eastwards in the Solomon Islands or Australia. Piper myrmecophilum, the only tax

  15. Philippines: Mobile Tent School for Selected Communities. Literacy Lessons.

    Science.gov (United States)

    Lasam, Gloria Z.

    The Philippines received the literacy award from Unesco in the 1986 International Literacy Day competition. The award was for the mobile tent school (MTS), an alternative school that can reach out to the most remote and deprived areas where groups of ethnic minorities exist whose cultures have resisted the lure of civilization. The nongraded MTS…

  16. New Thoughts on the Origins of Zamboangueno (Philippine Creole Spanish).

    Science.gov (United States)

    Lipski, John M.

    1992-01-01

    Attempts to reconcile the similarities and differences among Philippine Creole Spanish (PCS) dialects by suggesting that Zamboangueno was formed gradually in a downward fashion from received Spanish, aided by two components. The first is pidginization that resulted in the Spanish garrison at Zamboanga, and the second was the arrival of Manila Bay…

  17. Philippine Environmental Impact Assessment, Mining and Genuine Development

    Directory of Open Access Journals (Sweden)

    Allan Ingelson, William Holden & Meriam Bravante

    2009-06-01

    Full Text Available Genuine development reflects sustainability. To promote genuine development in the context of mining, the environmental impact assessment process in the Philippines needs to be changed to respect ecological integrity, mitigate cumulative environmental effects, provide more information on environmental impacts to residents affected by a proposed mine and facilitate meaningful public participation in the impact assessment process.

  18. Molecular detection and characterization of Theileria species in the Philippines.

    Science.gov (United States)

    Belotindos, Lawrence P; Lazaro, Jonathan V; Villanueva, Marvin A; Mingala, Claro N

    2014-09-01

    Theileriosis is a tick-borne disease of domestic and wild animals that cause devastating economic loss in livestock in tropical and subtropical regions. Theileriosis is not yet documented in the Philippines as compared to babesiosis and anaplasmosis which are considered major tick-borne diseases that infect livestock in the country and contribute major losses to the livestock industry. The study was aimed to detect Theileria sp. at genus level in blood samples of cattle using polymerase chain reaction (PCR) assay. Specifically, it determined the phylogenetic relationship of Theileria species affecting cattle in the Philippines to other Theileria sp. registered in the GenBank. A total of 292 blood samples of cattle that were collected from various provinces were used. Theileria sp. was detected in 43/292 from the cattle blood samples using PCR assay targeting the major piroplasm surface protein (MPSP) gene. DNA sequence showed high similarity (90-99%) among the reported Theileria sp. isolates in the GenBank and the Philippine isolates of Theileria. Phylogenetic tree construction using nucleotide sequence classified the Philippine isolates of Theileria as benign. However, nucleotide polymorphism was observed in the new isolate based on nucleotide sequence alignment. It revealed that the new isolate can be a new species of Theileria.

  19. Understanding electricity market reforms and the case of Philippine deregulation

    Energy Technology Data Exchange (ETDEWEB)

    Santiago, Andrea; Roxas, Fernando

    2010-03-15

    The experience of the Philippines offers lessons that should be relevant to any country seeking to deregulate its power industry. Regardless of structure, consumers must face the real price of electricity production and delivery that is closer to marginal cost. Politically motivated prices merely shift the burden from ratepayers to taxpayers. And any reform should work within a reasonable timetable. (author)

  20. An Analysis of the Communist Insurgency in the Philippines

    Science.gov (United States)

    2006-06-16

    insurgency situation in the Philippines from the Martial Law years until President Corazon Aquino took over. He believes that while Martial Law...2001), I-7. 54 CHAPTER 4 THE INSURGENCY (1987–DATE) A year after President Corazon Aquino assumed office in 1986 the CPP-NPA reached its peak

  1. The Philippine Press after Marcos: Restored Freedoms and New Problems.

    Science.gov (United States)

    Guimary, Donald L.

    With the overthrow of Ferdinand Marcos from his 20-year rule of the Philippines, the news media regained its freedom and its voice, and now faces a new set of problems: low circulation, questionable ethical standards of reporters and their lack of experience, and ominous indications from the Corazon Aquino government that the administration might…

  2. Geographies of Social Exclusion: Education Access in the Philippines

    Science.gov (United States)

    Symaco, Lorraine Pe

    2013-01-01

    Despite the massive education sector of the country, the Philippines--being located in the Pacific Ring of Fire and the typhoon belt--is home to a number of natural instabilities that frequently disrupt the school calendar. Conflict areas in selected parts of the island of Mindanao and the remote geographic features of the country also pose…

  3. Advancing Low Emission Growth in the Philippines (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    Watson, A.; Butheau, M.; Sandor, D.

    2013-11-01

    The Philippines, an island nation of nearly 100 million people and one of the world?s 15 most populous nations, is grappling with the challenge of expanding economic opportunities for its people. At the same time, it is one of the nations at greatest risk from climate change impacts, including rising sea levels and more frequent and intense floods and storms.

  4. Geographies of Social Exclusion: Education Access in the Philippines

    Science.gov (United States)

    Symaco, Lorraine Pe

    2013-01-01

    Despite the massive education sector of the country, the Philippines--being located in the Pacific Ring of Fire and the typhoon belt--is home to a number of natural instabilities that frequently disrupt the school calendar. Conflict areas in selected parts of the island of Mindanao and the remote geographic features of the country also pose…

  5. Vegetation types on Mount Akiki, Northern Luzon, Philippines

    NARCIS (Netherlands)

    Bout, I.E.

    2002-01-01

    Mount Akiki (16° 37’ N, 120° 53’ E, c. 2760 m alt.) is one of the highest mountain peaks in the Cordillera mountain range, Luzon Island, Philippines. It is situated in the municipality of Benguet, north-east of Baguio City (a world famous tourist city in the region) and is north-west of Mt Pulog, Lu

  6. The University of the Philippines College Baguio Herbarium

    NARCIS (Netherlands)

    Dogup-Balangcod, T.

    2001-01-01

    On 21 February 2000, the Discipline of Biology, University of the Philippines College Baguio opened its Herbarium to the public. It is the first local herbarium to be established in the entire Cordillera region of Luzon. The foundation was laid by collections made by me and other faculty members in

  7. International Briefing 26: Training and Development in the Philippines

    Science.gov (United States)

    Udani, Zenon Arthur S.; Sunio, Varsolo C.; Dado, Raul H.; Udani, Delia S.

    2012-01-01

    The Philippines has a population of more than 94 million. In addition, 10 million Filipinos work and live overseas. Filipino talent is ubiquitous overseas, working in offices, universities, hotels and restaurants, factories, shopping malls, theaters and arenas, and private homes around the world. The country's projected high rate of economic…

  8. Virginia Tech administrator receives award from Philippine government

    OpenAIRE

    2004-01-01

    Philippine President Gloria Macapagal Arroyo has honored S. K. De Datta of Blacksburg, associate provost for international affairs at Virginia Tech, with a Presidential Citation Award. The award recognizes De Datta's contributions towards eradicating hunger through improved agricultural productivity and food security, particularly for the production of rice.

  9. Piper (Piperaceae) in the Philippine Islands: the climbing species

    NARCIS (Netherlands)

    Gardner, R.O.

    2006-01-01

    Piper in the Philippine Islands is reviewed. Fifteen climbing species are recognized (many fewer than in previous treatments) and distinguished in a key. Most are widely distributed through Malesia, with ranges that end eastwards in the Solomon Islands or Australia. Piper myrmecophilum, the only

  10. Nepenthes ultra (Nepenthaceae), a new species from Luzon, Philippines

    NARCIS (Netherlands)

    Cheek, M.; Jebb, M.

    2013-01-01

    Nepenthes ultra is described from low altitude, ultramafic coastal scrub in Luzon, Philippines. It is placed in the Nepenthes alata group and is assessed as Endangered using the IUCN 2001 standard. Open cast mining and touristic development are threats to this species.

  11. A Composite Dictionary of Philippine Creole Spanish (PCS).

    Science.gov (United States)

    Riego de Rios, Maria Isabelita

    1989-01-01

    This dictionary is a composite of four Philippine Creole Spanish dialects: Cotabato Chabacano and variants spoken in Ternate, Cavite City, and Zamboanga City. The volume contains 6,542 main lexical entries with corresponding entries with contrasting data from the three other variants. A concluding section summarizes findings of the dialect study…

  12. 76 FR 60007 - TRICARE Demonstration Project for the Philippines

    Science.gov (United States)

    2011-09-28

    ..., controlling costs, eliminating any balance billing issues, and ensuring that the billing practices comply with... will no longer require payments at the time of service nor will they subject beneficiaries to balanced... Standard option in the Philippines, controlling costs, eliminating any balance billing issues, and...

  13. Oral History in the Philippines: Trends and Prospects.

    Science.gov (United States)

    Foronda, Marcelino A., Jr.

    1981-01-01

    Discusses oral history in precolonial and more recent times in the Philippines, with particular emphasis on the activities of the Filipino institutions and scholars engaged currently in oral history projects. Projects are classified by geographical location in the Metro Manila area, in the Visayas, and in Mindanao. (DB)

  14. PEACE CORPS PRIMER FOR THE WESTERN VISAYAS, PHILIPPINES.

    Science.gov (United States)

    ZORC, R. DAVID; AND OTHERS

    THIS TEXT WAS WRITTEN FOR PEACE CORPS VOLUNTEERS WORKING IN AREAS OF PANAY AND THE ROMBLON ISLANDS IN THE PHILIPPINES. COVERED IN THIS SHORT TEXT ARE "ALL THE DIALECTS OF THE WESTERN VISAYAS" (EXCEPT THE DIALECTS SPOKEN BY THE NEGRITOS)--AKLANON, KINARAY-A, CAPIZNON, ILONGO, LOOCNON, ODIONGANON, AND ROMBLOMANON. IN SPITE OF DIFFERENCES AMONG THESE…

  15. A new Stenolemus from the Philippines (Heteroptera, Reduviidae, Emesinae)

    NARCIS (Netherlands)

    Doesburg, van P.H.

    1975-01-01

    During investigations of fig-wasps in the Philippines in 1964, Dr. J. T. Wiebes collected a new species of bug, a small reduviid belonging to the genus Stenolemus Signoret: its description is presented here. For comparison, the type specimen of Stenolemus crassirostris Stâl, 1870 (similarly from the

  16. The Rhynchium species of the Philippine Islands (Hymenoptera, Eumenidae)

    NARCIS (Netherlands)

    Vecht, van der J.

    1968-01-01

    It has generally been accepted that the Philippine Islands are inhabited by only a single species of the Eumenid genus Rhynchium. In Miss Baltazar's valuable catalogue (1966) it is listed as a subspecies, atrum Saussure, of R. haemorrhoidale (Fabr.). However, when in 1963 I examined an extensive col

  17. Counseling in the Philippines: Past, Present, and Future

    Science.gov (United States)

    Tuason, Ma. Teresa G.; Galang Fernandez, Karina Therese; Catipon, Maria Aurora D. P.; Trivino-Dey, Louise; Arellano-Carandang, Ma. Lourdes

    2012-01-01

    The history of colonization and persistent attempts at self-governance in the Philippines parallel the beginnings of counseling in the country, which were largely influenced by the United States. Because of the Guidance and Counseling Act of 2004 and the Psychology Act of 2009, counseling is on its way to professionalization and regulation.…

  18. 78 FR 22237 - Trade Mission to Philippines and Malaysia

    Science.gov (United States)

    2013-04-15

    ... sites, press releases to general and trade media, direct mail, notices by industry trade associations... International Trade Administration Trade Mission to Philippines and Malaysia AGENCY: International Trade... Commerce, International Trade Administration, U.S. & Foreign Commercial Service (US&FCS), is organizing an...

  19. Forensic DNA evidence and the death penalty in the Philippines.

    Science.gov (United States)

    De Ungria, M C A; Sagum, M S; Calacal, G C; Delfin, F C; Tabbada, K A; Dalet, M R M; Te, T O; Diokno, J I; Diokno, M S I; Asplen, C A

    2008-09-01

    The death penalty remains a contentious issue even though it has been abolished in countries such as Australia, New Zealand, Canada, European Union member nations and some Asian countries such as Cambodia, East Timor and Nepal. Many argue that the irrevocability of the death penalty, in the face of potential erroneous convictions, can never justify its imposition. The Philippines, the first Asian country that abolished the death penalty in 1987, held the record for the most number of mandatory death offenses (30 offenses) and death eligible offenses (22 offenses) after it was re-imposed in 1994. Majority of death penalty convictions were decided based on testimonial evidence. While such cases undergo automatic review by the Supreme Court, the appellate process in the Philippines is not structured to accept post-conviction evidence, including DNA evidence. Because of the compelling nature of post-conviction DNA evidence in overturning death penalty convictions in the United States, different groups advocated its use in the Philippines. In one such case, People v Reynaldo de Villa, the defendant was charged with raping his 13-year-old niece that supposedly led to birth of a female child, a situation commonly known as 'criminal paternity'. This paper reports the results of the first post-conviction DNA test using 16 Short Tandem Repeat (STR) DNA markers in a criminal paternity case (People v Reynaldo de Villa) and discusses the implications of these results in the Philippine criminal justice system.

  20. 冰壶运动员致胜心理因素探析%Analysis on Winning Psychological Factors of Curling Athletes

    Institute of Scientific and Technical Information of China (English)

    史孟玉; 姜淼淼

    2014-01-01

    针对我国冰壶运动发展的优势和存在的问题,抓住冰壶运动独具的项目特征,分析冰壶运动员致胜心理因素,加快提升我国冰壶运动整体竞技能力,力争在索契冬奥会上取得佳绩,是我国冰壶运动教练员、运动员亟待解决的重要课题。良好的心理状态是冰壶运动员最佳竞技状态形成的重要因素。从团队精神、动机、心理准备、自信心、注意力和焦虑控制等方面探究冰壶运动员致胜心理因素,旨在寻求提高冰壶运动训练水平、提升冰壶运动员整体竞技能力和冰壶运动员在比赛中取得优异成绩的突破口。%Aiming at the advantages and problems of Chinese curling development,stressing the unique characteristics of curling,analyzing the winning psychological factors of curling athletes, improving the overall competitive ability of Chinese curling and striving for good performance in Olympics in Sochi is the important task which should be solved quickly by Chinese curling coaches and athletes. A good mental state is the important factor of curling athletes being at their best competitive form. Analyze the winning psychological factors of curling athletes from team spirit, motivation,psychological preparation, self-confidence,attention and anxious control,in order to seek a breach which improves training level of curling,improve the overall competitive ability of curling athletes and get good performance in competitions.

  1. Epidemiology of drowning deaths in the Philippines, 1980 to 2011

    Directory of Open Access Journals (Sweden)

    Rammell Eric Martinez

    2016-11-01

    Full Text Available Drowning kills 372 000 people yearly worldwide and is a serious public health issue in the Philippines. This study aims to determine if the drowning death rates in the Philippine Health Statistics (PHS reports from 1980 to 2011 were underestimated. A retrospective descriptive study was conducted to describe the trend of deaths caused by drowning in the Philippines from official and unofficial sources in the period 1980 to 2011. Information about deaths related to cataclysmic causes, particularly victims of storms and floods, and maritime accidents in the Philippines during the study period were reviewed and compared with the PHS drowning death data. An average of 2496 deaths per year caused by drowning were recorded in the PHS reports from 1980 to 2011 (range 671-3656. The average death rate was 3.5/100 000 population (range 1.3-4.7. An average of 4196 drowning deaths were recorded from 1980 to 2011 (range 1220 to 8788 when catacylsmic events and maritime accidents were combined with PHS data. The average death rate was 6/100 000 population (range 2.5-14.2. Our results showed that on average there were 1700 more drowning deaths per year when deaths caused by cataclysms and maritime accidents were added to the PHS data. This illustrated that drowning deaths were underestimated in the official surveillance data. Passive surveillance and irregular data management are contributing to underestimation of drowning in the Philippines. Additionally, deaths due to flooding, storms and maritime accidents are not counted as drowning deaths, which further contributes to the underestimation. Surveillance of drowning data can be improved using more precise case definitions and a multisectoral approach.

  2. Vector wind, horizontal divergence, wind stress and wind stress curl from SEASAT-SASS at one degree resolution

    Science.gov (United States)

    Pierson, W. J., Jr.; Sylvester, W. B.; Salfi, R. E.

    1984-01-01

    Conventional data obtained in 1983 are contrasted with SEASAT-A scatterometer and scanning multichannel microwave radiometer (SMMR) data to show how observations at a single station can be extended to an area of about 150,000 square km by means of remotely sensed data obtained in nine minutes. Superobservations at a one degree resolution for the vector winds were estimated along with their standard deviations. From these superobservations, the horizontal divergence, vector wind stress, and the curl of the wind stress can be found. Weather forecasting theory is discussed and meteorological charts of the North Pacific Ocean are presented. Synoptic meteorology as a technique is examined.

  3. The Philippines, the East Asian "Developmental States" and Education: A Comparative Analysis of Why the Philippines Failed to Develop

    Science.gov (United States)

    Maca, Mark; Morris, Paul

    2012-01-01

    After WWII, the economic prospects of the Philippines, then the second-largest economy in Asia, were viewed positively, but by the mid-1970s it had become Asia's developmental puzzle for its failure to sustain economic growth. In contrast during the same period, regional neighbours, Hong Kong, Taiwan, South Korea and Singapore, achieved previously…

  4. Vertical Movements, Behavior and Habitat Utilization of Adult Yellowfin Tuna (Thunnus albacares) Tagged in West Philippine Sea, Mindoro Island, Philippines

    NARCIS (Netherlands)

    Macusi, Edison; Babaran, R.P.; Ingles, J.T.

    2015-01-01

    This paper describes the vertical movements, behavior and habitat utilization of an electronically tagged adult yellowfin tuna (Thunnus albacares) after 17 days of liberty in the Philippines based on analyses of 23,845 datapoints recorded from a recovered electronic popup tag. The exhaustive set pro

  5. Vertical Movements, Behavior and Habitat Utilization of Adult Yellowfin Tuna (Thunnus albacares) Tagged in West Philippine Sea, Mindoro Island, Philippines

    NARCIS (Netherlands)

    Macusi, Edison; Babaran, R.P.; Ingles, J.T.

    2015-01-01

    This paper describes the vertical movements, behavior and habitat utilization of an electronically tagged adult yellowfin tuna (Thunnus albacares) after 17 days of liberty in the Philippines based on analyses of 23,845 datapoints recorded from a recovered electronic popup tag. The exhaustive set

  6. Foraging behaviour and landscape utilisation by the endangered golden-crowned flying fox (Acerodon jubatus, the Philippines.

    Directory of Open Access Journals (Sweden)

    Carol de Jong

    Full Text Available Species of Old World fruit-bats (family Pteropodidae have been identified as the natural hosts of a number of novel and highly pathogenic viruses threatening livestock and human health. We used GPS data loggers to record the nocturnal foraging movements of Acerodon jubatus, the Golden-crowned flying fox in the Philippines to better understand the landscape utilisation of this iconic species, with the dual objectives of pre-empting disease emergence and supporting conservation management. Data loggers were deployed on eight of 54 A. jubatus (two males and six females captured near Subic Bay on the Philippine island of Luzon between 22 November and 2 December 2010. Bodyweight ranged from 730 g to 1002 g, translating to a weight burden of 3-4% of bodyweight. Six of the eight loggers yielded useful data over 2-10 days, showing variability in the nature and range of individual bat movements. The majority of foraging locations were in closed forest and most were remote from evident human activity. Forty-six discrete foraging locations and five previously unrecorded roost locations were identified. Our findings indicate that foraging is not a random event, with the majority of bats exhibiting repetitious foraging movements night-to-night, that apparently intact forest provides the primary foraging resource, and that known roost locations substantially underestimate the true number (and location of roosts. Our initial findings support policy and decision-making across perspectives including landscape management, species conservation, and potentially disease emergence.

  7. An HIV epidemic is ready to emerge in the Philippines

    Directory of Open Access Journals (Sweden)

    Farr Anna C

    2010-04-01

    Full Text Available Abstract Background The state of the HIV epidemic in the Philippines has been described as "low and slow", which is in stark contrast to many other countries in the region. A review of the conditions for HIV spread in the Philippines is necessary. Methods We evaluated the current epidemiology, trends in behaviour and public health response in the Philippines to identify factors that could account for the current HIV epidemic, as well as to review conditions that may be of concern for facilitating an emerging epidemic. Results The past control of HIV in the Philippines cannot be attributed to any single factor, nor is it necessarily a result of the actions of the Filipino government or other stakeholders. Likely reasons for the epidemic's slow development include: the country's geography is complicated; injecting drug use is relatively uncommon; a culture of sexual conservatism exists; sex workers tend to have few clients; anal sex is relatively uncommon; and circumcision rates are relatively high. In contrast, there are numerous factors suggesting that HIV is increasing and ready to emerge at high rates, including: the lowest documented rates of condom use in Asia; increasing casual sexual activity; returning overseas Filipino workers from high-prevalence settings; widespread misconceptions about HIV/AIDS; and high needle-sharing rates among injecting drug users. There was a three-fold increase in the rate of HIV diagnoses in the Philippines between 2003 and 2008, and this has continued over the past year. HIV diagnoses rates have noticeably increased among men, particularly among bisexual and homosexual men (114% and 214% respective increases over 2003-2008. The average age of diagnosis has also significantly decreased, from approximately 36 to 29 years. Conclusions Young adults, men who have sex with men, commercial sex workers, injecting drug users, overseas Filipino workers, and the sexual partners of people in these groups are particularly

  8. Screening low fire blight susceptible Crataegus species for host suitability to hawthorn leaf-curling aphids (Dysaphis spp.).

    Science.gov (United States)

    Bribosia, E; Bylemans, D; Van Impe, G; Migon, M

    2002-01-01

    The group of hawthorn leaf-curling aphids (Dysaphis spp.) hosted by the common hawthorn Crataegus monogyna Jacq. may play an important role in the biological control of the rosy apple aphid, Dysaphis plantaginea (Passerini), by increasing reproduction opportunities for the indigenous hymenopteran parasitoid Ephedrus persicae Froggatt. Unfortunately, most fruitgrowers hesitate to introduce the common hawthorn in their orchards because they fear fire blight infections which may be transmitted by this highly susceptible hawthron species. This potential hazard led us to investigate the suitability to leaf-curling aphids of alternative Crataegus species. As representative for these closely-related aphids, the species Dysaphis apiifolia petroselini (Börner) was used in the trials. Ten Crataegus species characterized by their very low susceptibility to fire blight were examined from two angles. Firstly, aphid sexuals were introduced in autumn onto the different species to verify whether egg laying could take place. Secondly, the development of fundatrices and gall formation were followed the next spring. Although eggs and mature fundatrices could be obtained on almost all species, no fundatrice-hosting galls were recorded in spring. The possible causes of these negative results with respect to the geographical origin of the particular Crataegus species involved in this work are discussed.

  9. ENSO-correlated fluctuations in ocean bottom pressure and wind-stress curl in the North Pacific

    Directory of Open Access Journals (Sweden)

    D. P. Chambers

    2011-07-01

    Full Text Available We examine the magnitude of ENSO-correlated variations in wind-stress curl and ocean bottom pressure in the North Pacific between 1992 until 2010, using satellite observations and model output. Our analysis indicates that while there are significant fluctuations correlated with some El Niño and La Niña events, the correlation is still relatively low. Moreover, the ENSO-correlated variability explains only 50 % of the non-seasonal, low-frequency variance. There are significant residual fluctuations in both wind-stress curl and ocean bottom pressure in the region with periods of 4-years and longer. One such fluctuation began in late 2002 and has been observed by the Gravity Recovery and Climate Experiment (GRACE. Even after accounting for ENSO variations, there is a significant trend in ocean bottom pressure in the region, equivalent to 0.7 ± 0.3 cm yr−1 of sea level from January 2003 until December 2008, which is confirmed with steric-corrected altimetry. Although this low-frequency fluctuation does not appear in an ocean model, we show that the winds used to force the model have a significantly reduced trend that is inconsistent with satellite observations over the same time period.

  10. Method of inhibiting plant virus pathogen infections by crispr/cas9-mediated interference

    KAUST Repository

    Mahfouz, Magdy Mahmoud

    2016-11-24

    A genetically modified tobacco plant or tomato plant resistant to at least one pathogenic geminiviridae virus species is provided. The plant comprises a heterologous CRISPR/Cas9 system and at least one heterologous nucleotide sequence that is capable of hybridizing to a nucleotide sequence of the pathogenic virus and that directs inactivation of the pathogenic virus species or plurality of viral species by the CRISPR/Cas9 system. The heterologous nucleotide sequence can be complementary to, but not limited to an Intergenic Region (IR) of the Tomato Yellow Leaf Curl Virus (TYLCV), Further provided are methods of generating a genetically modified plant that is resistant to a virus pathogen by a heterologous CRISPR/Cas9 system and expression of a gRNA specifically targeting the virus.

  11. 38 CFR 17.367 - Republic of the Philippines to print forms.

    Science.gov (United States)

    2010-07-01

    ... 38 U.S.C. 1724 and 1732, and 38 CFR 17.36 through 17.40 and §§ 17.350 through 17.370. The forms will... Philippines to print forms. 17.367 Section 17.367 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS MEDICAL Grants to the Republic of the Philippines § 17.367 Republic of the Philippines...

  12. Determinants of International Tourism Demand for the Philippines: An Augmented Gravity Model Approach

    OpenAIRE

    Deluna, Roperto Jr; Jeon, Narae

    2014-01-01

    This study was conducted to investigate the determinants of international tourism demand for the Philippines.This study employed a double-log augmented form of gravity model estimated using the robust random effects model.Results revealed that tourist arrival in the Philippines are generally increasing from 2001 to 2012. Empirical estimation was conducted to determine factors affecting Philippine tourism demand. These factors include income, market size, and distance. Relative prices was also...

  13. Disease Burden of Dengue in the Philippines: Adjusting for Underreporting by Comparing Active and Passive Dengue Surveillance in Punta Princesa, Cebu City.

    Science.gov (United States)

    Undurraga, Eduardo A; Edillo, Frances E; Erasmo, Jonathan Neil V; Alera, Maria Theresa P; Yoon, In-Kyu; Largo, Francisco M; Shepard, Donald S

    2017-04-01

    AbstractDengue virus (DENV) is a serious threat to public health. Having reliable estimates of the burden of dengue is important to inform policy and research, but surveillance systems are not designed to capture all symptomatic DENV infections. We derived the rate of reporting of dengue by comparing active surveillance of symptomatic DENV infections in a prospective community-based seroepidemiological cohort study (N = 1008) of acute febrile illness in Punta Princesa, Cebu City, Philippines, with passive surveillance data from the Cebu City Health Department. Febrile episodes detected in a weekly follow-up of participants were tested for serotype-specific DENV by hemi-nested reverse transcription-polymerase chain reaction (nested RT-PCR) and acute/convalescent blood samples tested by dengue IgM/IgG enzyme immunoassay. We estimated the burden of dengue in the Philippines in disability-adjusted life years (DALYs), and conducted a probabilistic sensitivity analysis using Monte-Carlo simulations to address uncertainty. The results showed a 21% cumulative reporting rate of symptomatic DENV infections, equivalent to an expansion factor of 4.7 (95% certainty level [CL]: 2.2-15.1). Based on surveillance data in the Philippines for 2010-2014, we estimated 794,255 annual dengue episodes (95% CL: 463,000-2,076,000) and a disease burden of 535 (95% CL: 380-994) DALYs per million population using age weights and time discounting and 997 (95% CL: 681-1,871) DALYs per million population without age and time adjustments. Dengue imposes a substantial burden in the Philippines; almost 10 times higher than estimated for rabies, about twice the burden of intestinal fluke infections, and about 10% of the burden of tuberculosis. Our estimates should inform policy makers and raise awareness among the public.

  14. Determination of Euler parameters of Philippine Sea plate and the inferences

    Institute of Scientific and Technical Information of China (English)

    臧绍先; 陈起永; 宁杰远; 沈正康; 刘永刚

    2002-01-01

    Euler vectors of 12 plates, including Philippine Sea plate (PH), relative to a randomly fixed Pacific plate(PA) were determined by inverting the 1122 data from NUVEL-1 global plate motion model, earthquake slip vectors along Philippine Sea plate boundary, and GPS observed velocities. Euler vectors of Philippine Sea plate relative to adjacent plates are also gained. Our results are well consistent with observed data and can satisfy the geological and geophysical constraints along the Caroline(CR)-PH and PA-CR boundaries. Deformation of Philippine Sea plate is also discussed by using the plate motion Euler parameters.

  15. Fontes de resistência em tomateiro aos begomovírus bissegmentados Tomato yellow spot virus e Tomato severe rugose virus

    OpenAIRE

    2012-01-01

    Os begomovírus causam doenças de grande importância econômica em diversas culturas, principalmente em regiões tropicais e subtropicais. Juntamente com outras famílias de vírus, os begomovírus têm causado grande prejuízo para os produtores de tomate in natura e para processamento industrial. O objetivo deste trabalho foi avaliar o comportamento de 11 genótipos resistentes ao Tomato yellow leaf curl virus (TYLCV) frente à infecção pelos begomovírus Tomato yellow spot virus (ToYSV) e Tomato seve...

  16. Lessons From Early REDD+ Experiences in the Philippines

    Directory of Open Access Journals (Sweden)

    Rodel D. Lasco

    2013-01-01

    Full Text Available There is growing interest globally in REDD+ initiatives to help mitigate climate change; the Philippines is no exception. In this paper, we review early REDD+ project experiences in the country. The guiding document for REDD+ in the Philippines is the National REDD+ Strategy (PNRPS which was prepared by a multisectoral group of authors. There are five REDD+ projects that are underway. The critical factors emerging from these early REDD+ actions are the following: external support, local participation, free prior and informed consent, capacity building, sustainability, national laws and policies, biodiversity conservation, and use of safeguards. The pioneering projects reviewed here as well as the emerging lessons from them will hopefully provide a firmer basis for future REDD+ actions in the country.

  17. State of Art and Knowledgeof Urban Forestry in the Philippines

    Institute of Scientific and Technical Information of China (English)

    PALIJONArmandoManito

    2005-01-01

    Urban forestry is an emerging science and art in the field of resource management in the Philippines.The concepts and principles are quite similar with traditional forestry being both resource management strategies. As a science and art, urban forestry needs to be assessed as to its present status. Hence, in this paper, I presented a brief historical background about urban forestry in the Philippines including the previous and present efforts, policies and programs related to urban forestry and the extent of their implementation.I also included the result of my assessment about the initiatives and capabilities of the local agencies specifically the local government units (LGUs) in Metro Manila on urban forestry. I also included the status of urban forestry research including the research gaps that need to be addressed. Some recommendations that are logical toward sustainability of urban green spaces are likewise enumerated.

  18. Modernity vs. Culture: Protecting the Indigenous Peoples of the Philippines

    Directory of Open Access Journals (Sweden)

    Michael Teodoro G. Ting Jr.

    2016-01-01

    Full Text Available The indigenous peoples of the Philippines (IPs held a distinct culture before the arrival of the Spanish, American, and Japanese colonizers in the archipelago. Once, they were original settlers with revered customs and rituals. Over time, the IPs evolved into a minority group of decreasing social, economic, and political power. Gradually, they transformed into one of the most ignored sectors of Philippine society. Progress looked down on their civilization. Technology threatened their traditions. Modernity infringed on their rights. This paper examines the plight of the IPs using historical, cultural, legal, and political viewpoints. It shall gauge their current situation, and recommend viable ways to improve their present condition and secure their future.

  19. STR polymorphisms in Philippine ethnolinguistic groups: evaluation of forensic utility.

    Science.gov (United States)

    Miranda, Jasmin Jiji; Halos, Saturnina C

    2004-01-01

    Population data was collected for the STR loci F13AO1, FES/FPS, HUMvWA, and HUMTHO1, in three major Philippine ethnolinguistic groups and used to estimate statistical parameters for identity testing in forensic work on Filipinos. The Cebuano, Ilocano, and Pampango populations in the Philippines were studied because they are among the biggest linguistic groups in the country, thus their genotypic profiles should substantially represent those of many Filipinos. The number of alleles varied from 4 to 9 at all loci, falling within the range observed for other local and world populations. Pairwise comparisons of the allele frequency distributions showed no statistical differences among the populations. The test for linkage equilibrium showed no evidence of non-random association of alleles across the physically unlinked loci in any of the three populations. The four loci combined gave an exclusion power of > or =0.9995 and a power of paternity exclusion of 0.8859-0.9389.

  20. Traditional animal disease control methods in the Philippines.

    Science.gov (United States)

    Topacio, T M; Jovellanos, M L

    1994-06-01

    The authors present a number of animal disease control methods practised in the Philippines which have been handed down from generation to generation. Data were obtained through questionnaires, personal interviews and a literature search. Most of the data collected refer to the Cordillera Autonomous Region. Rituals, incantations and prayers to ward off evil spirits were the most popular practices in the mountain provinces of this region. Sacrifice of animals to appease evil spirits, and thanksgiving rituals to improve fertility and production were also common. In addition, evil spirits may be driven away by burning incense or herbs, tying a red ribbon round the necks of animals or fencing off the perimeter of a pen or corral. A total of fifteen traditional practices is reported. Other indigenous practices worthy of note may also occur within the Muslim and Visayan communities in central and southern areas of the Philippines.

  1. Modernity vs. Culture: Protecting the Indigenous Peoples of the Philippines

    Directory of Open Access Journals (Sweden)

    Michael Teodoro G. Ting Jr.

    2008-05-01

    Full Text Available The indigenous peoples of the Philippines (IPs held a distinct culture before the arrival of the Spanish, American, and Japanese colonizers in the archipelago. Once, they were original settlers with revered customs and rituals. Over time, the IPs evolved into a minority group of decreasing social, economic, and political power. Gradually, they transformed into one of the most ignored sectors of Philippine society. Progress looked down on their civilization. Technology threatened their traditions. Modernity infringed on their rights. This paper examines the plight of the IPs using historical, cultural, legal, and political viewpoints. It shall gauge their current situation, and recommend viable ways to improve their present condition and secure their future.

  2. Tourism and Crime: Evidence from the Philippines

    OpenAIRE

    Palanca-Tan, Rosalina; Garces, Len Patrick Dominic M.; Angelica Nicole C. Purisima; Zaratan, Angelo Christian L.

    2015-01-01

    Using panel data gathered from 16 regions of the Philippines for the period 2009–11, this paper investigates the relationship between tourism and crime. The findings of the study show that the relation between tourism and crime may largely depend on the characteristics of visitors and the types of crime. For all types of crime and their aggregate, no significant correlation between the crime rate (defined as the number of crime cases divided by population) and total tourist arrivals is found....

  3. US Influence on Military Professionalism in the Philippines

    Science.gov (United States)

    2003-12-01

    formed and led by Colonel “Gringo” Honasan. This group of military officers rallied under the leadership of Defense Minister Juan Ponce Enrile to oust... Reyes spearheaded the military intervention by shifting his support to Macapagal-Arroyo. The January 2001...for designating the Philippines as a “Major Non-Nato Ally.” As the new Commander-in-Chief, Macapal-Arroyo appointed General Angelo Reyes as

  4. Philippines and Indonesia: on the way to a migration transition.

    Science.gov (United States)

    Amjad, R

    1996-01-01

    "This paper, in a comparative analysis of the Philippines and Indonesia, examines first under what conditions can migration favorably contribute to the process of economic development and then to what extent can economic growth impact upon reducing emigration pressures in these labor surplus economies. The paper also argues that there is still considerable scope for putting in place [an] agreed set of rules and policies to ensure better protection for the more vulnerable migrants."

  5. Structurally controlled hazard mapping of Southern Leyte, Philippines

    Science.gov (United States)

    Luzon, P. K.; Montalbo, K. P.; Galang, J. A. M.; Sabado, J. M.; Escape, C. M.; Felix, R. P.; Lagmay, A. M. F.

    2015-10-01

    The 2006 Guinsaugon landslide in St. Bernard, Southern Leyte is one of the largest known landslides in the Philippines in recent history. It consists of a 15-20 million m3 rockslide-debris avalanche from an approximately 675 m high mountain weakened by continuous movement of the Philippine fault. The catastrophic Guinsaugon landslide killed 1221 people and displaced 19 000 residents over its 4.5 km path. To investigate the present day morphology of the scar and potential failure that may occur, analysis of a 5 m resolution IfSAR-derived Digital Elevation Model was conducted using Coltop3D and Matterocking software, leading to the generation of a landslide hazard map for the province of Southern Leyte in Central Philippines. The dip and dip-direction of discontinuity sets that contribute to gravitational failure in mountainous areas of the province were identified and measured using a lower Schmidt-Lambert color scheme. After measurement of the morpho-structural orientations, potential sites of failure were analyzed. Conefall was then utilized to compute the extent of rock mass runout. Results of the analysis show instability in the scarp area of the 2006 Guinsaugon landslide and in adjacent slopes because of the presence of steep discontinuities that range from 45-60°. Apart from the 2006 Guinsaugon landslide site, runout models simulated farther rock mass extent in its adjacent slopes, revealing a high potential for fatal landslides to happen in the municipality of St. Bernard. Concerned agencies may use maps produced in the same manner as this study to identify possible sites where structurally-controlled landslides can occur. In a country like the Philippines, where fractures and faults are common, this type of simulated hazard maps would be useful for disaster prevention and facilitate disaster risk reduction efforts for landslide-susceptible areas.

  6. Assessment of Solid Waste Management Strategies in Camarines Norte, Philippines

    OpenAIRE

    Maria Cristina C. Azuelo; Leah N. Barbado; Luz Menda L. Reyes

    2016-01-01

    The Ecological Solid Waste Management Act of 2000 or RA 9003 mandates the local government units to take initiatives in managing their daunting problems on ecological solid waste disposal. Consequently, compliance of Camarines Norte, Philippines on this mandate needs assessment to determine the existing solid waste management (SWM) strategies, the effectiveness and the possibility of adoption in each municipality. This study utilized the descriptive method using questionnaire as t...

  7. Forward Deployed Naval Forces in the Republic of the Philippines

    Science.gov (United States)

    2016-06-10

    Department of State, accessed April 5, 2016, http://www.state.gov/secretary/remarks/2013/12/218869.htm. 5 Eliza Gray, “U.S. Marine Suspected in...information about the advantages and disadvantages was available. In conducting research, the reliance of professional experience from other naval...Periodicals Gray, Eliza . “U.S. Marine Suspected in Killing of Transgender Woman in Philippines.” Time Magazine, October 14, 2014. Accessed April

  8. Resolving Questioned Paternity Issues Using a Philippine Genetic Database

    Directory of Open Access Journals (Sweden)

    Maria Corazon De Ungria

    2002-06-01

    Full Text Available The utility of the Philippines genetic database consisting of seven Short Tandem Repeat (STR markers for testing of ten questioned paternity cases was investigated. The markers used were HUMvWA, HUMTH01, HUMCSF1PO, HUMFOLP23, D8S306, HUMFES/FPS, and HUMF13A01. These markers had a combined Power of Paternity Exclusion of 99.17%. Due to the gravity of some cases handled in the laboratory, routine procedures must be assessed to determine the capacity of the analysis to exclude a non-father of predict paternity. Clients showed a preference for only testing father and child to lower costs and reduce conflicts, particularly when the mother objects to the conduct of DNA tests, or when she is deceased or cannot be located. The Probability of Paternity was calculated with and without the mother’s profile in each of the cases. In all instances, results were more informative when the mother’s DNA profile was included. Moreover, variations in the allelic distribution of five STR markers among eight Caucasian, one African-American, and two Amerindian (Argentina populations resulted in significant differences in Probability of Paternity estimates compared to those calculated using the Philippine Database.Based on the results of the present study, it is recommended that tests on alleged father-child samples be performed to screen for at least two mismatches. In the absence of theses mismatches, further analysis that includes the mother’s DNA profile is recommended. Moreover, it is recommended that a Philippines genetic database be used for DNA-based paternity testing in the Philippines.

  9. Burgos Wind Farm Project : The Philippines Technical Desk Review

    OpenAIRE

    Cronin, Tom; Clausen, Niels-Erik; Nielsen, Morten; Jørgensen, Erik

    2012-01-01

    This report presents the findings of a review carried out by Riso National Laboratory of a 30MW wind farm in Northern Luzon, The Philippines. The review was commissioned by the World Bank and is intended as a brief second opinion desk review of the technical, contractual and financial aspects of the project. The evaluation of the Annual Energy Production of the site by PNOC-EDC seems to fo...

  10. Multiple infections of Anaplasma platys variants in Philippine dogs

    Directory of Open Access Journals (Sweden)

    Adrian Patalinghug Ybañez

    2016-12-01

    Full Text Available Aim: Anaplasma platys, the causative agent of infectious canine cyclic thrombocytopenia, is a tick-borne pathogen that also has been implicated as potentially zoonotic. To provide molecular evidence on the multiple infections of A. platys variants in Philippine dogs. Materials and Methods: DNA fragments of A. platys from infected dogs in the Philippines were molecularly characterized. For screening, 25 dogs suspected to have canine anaplasmosis were tested using a 16S rRNA-based nested polymerase chain reaction (PCR. Infection was confirmed by sequencing of positive amplicons. Second round PCR targeting a longer 16S rRNA fragment was subsequently performed on the first round PCR amplicons of the positive samples. Further characterization using the heat-shock operon (groEL gene was also performed on the A. platys-positive samples. Results: 10 16S rRNA sequences were obtained and found 99.6-100% identical to each other and 99.6-99.7% identical to the closest registered A. platys sequences. On the other hand, 36 groEL clone sequences were obtained and found to be 85.1-99.8% identical with each other and 85.0-88.9% identical to the closest previously registered A. platys sequences. Four dogs were found coinfected with 2-3 groEL variant sequences. Phylogenetic analyses suggest that the detected A. platys in the Philippines may represent unique variants. Conclusion: A. platys variants were detected in Philippine dogs. Coinfection of different A. platys variants in dogs was also demonstrated. The present study may indicate the potential genetic diversity of A. platys in the country.

  11. Basic Study on Flood Management Assessment in Metro Manila, Philippines

    OpenAIRE

    Romeo Libunao, Gilbuena Jr

    2013-01-01

    Flooding is the most frequent and damaging natural hazard worldwide. The resulting impact of flood disasters on society depends on the economic strength of the affected country prior to the disaster. The larger the disaster and the smaller the economy, the more significant is the impact. This is very clearly seen in developing countries, like the Philippines, where weak economies become much weaker after a devastating flood event. In 2009, tropical storm Ondoy, brought heavy rainfalls that pr...

  12. “Successes” and Adjustment in the Philippine Labor Market

    OpenAIRE

    Gerardo P. Sicat

    2004-01-01

    A high degree of unemployment and underemployment characterizes the Philippine economic development record. In large part, the mainstream labor policies that emphasized high labor welfare standards and high minimum manages over the creation of employment are responsible for this outcome. In spite of this poor record of employment generation, some successes have been achieved in generating jobs and incomes in specific areas of industry and services. Focusing on these successes gives an insight...

  13. A NEW SPECIES OF CHRYSODEMA FROM MINDORO, PHILIPPINES (COLEOPTERA, BUPRESTIDAE

    Directory of Open Access Journals (Sweden)

    Maurizio Gigli

    2007-10-01

    Full Text Available Chrysodema danterina n. sp. from Mindoro, Philippines, is described and illustrated. Comparative remarks are made with the widely distributed Chrysodema smaragdu­la (Olivier, 1790 that, despite a very different general look, shows several similar characters. The new species, whose female is unknown, is immediately recognizable from any other in the genus for the proportions of the body, the completely black colour and the shape and the exceptional size of male genitalia.

  14. Philippine Counterinsurgency Success: Implications for the Human Domain of Warfare

    Science.gov (United States)

    2013-03-01

    And if it is indeed new and distinct from the land domain, inanimate and geographic, we in our Nation’s Army should be preparing ourselves to...different ethnic, cultural, and religious backgrounds. The post-World War II Philippine government has been no stranger to internal conflict...environments. We must identify how we get ourselves to where we need to be in order to best influence outcomes, to include better applying the tools we

  15. Infectivity analysis of a blackgram isolate of Mungbean yellow mosaic virus and genetic assortment with MYMIV in selective hosts.

    Science.gov (United States)

    Haq, Q M I; Rouhibakhsh, A; Ali, Arif; Malathi, V G

    2011-06-01

    Yellow mosaic disease in grain legumes in Indian subcontinent is caused by two important virus species viz. Mungbean yellow mosaic virus (MYMV) and Mungbean yellow mosaic India virus (MYMIV), belonging to the genus Begomovirus of the family Geminiviridae. The genomic components of a begomovirus causing yellow mosaic disease in blackgram in southern India were cloned and sequenced. Nucleotide sequence comparison of DNA A component shows the virus isolate to be a variant of Mungbean yellow mosaic virus:-(MYMV-[IN:Vam:05]). However, DNA B component of the present virus isolate has greater similarity (92%) to Mungbean yellow mosaic India virus. Agroinoculations of the viral clones produced typical yellow mosaic symptoms in blackgram and mungbean, severe leaf curl and stunting in French bean, similar to blackgram isolate of MYMIV. Blackgram isolates of both the virus species were only mildly infectious on cowpea, produced atypical leaf curl symptoms and not yellow or golden mosaic. In agroinoculations done by exchanging genomic components, symptom expression was seen only in French bean. In cowpea, blackgram and mungbean there was no visible symptoms though viral DNA could be detected by PCR.

  16. Malvastrum yellow vein virus,a new Begomovirus species associated with satellite DNA molecule

    Institute of Scientific and Technical Information of China (English)

    ZHOU Xueping; XIE Yan; PENG Yan; ZHANG Zhongkai

    2003-01-01

    Virus isolate Y47 was obtained from Malvastrum coromandelianum showing yellow vein symptom in Honghe, Yunnan Province. The complete nucleotide sequence of DNA-A was determined, it contains 2731 nucleotides, having typical genomic organization of a begomovirus, encoding 6 ORFs with 2 ORFs [AV1(CP) and AV2] in virion- sense DNA and 4 ORFs (AC1-AC4) in complementary- sense DNA. Comparisons show that the total DNA-A of Y47 has the highest sequence identity (77%) with that of Okra yellow vein mosaic virus-[201] (AJ002451), while less than 76% identities are found when compared with other begomoviruses. The molecular data show that virus isolate Y47 is a distinct begomovirus species, for which the name Malvastrum yellow vein virus is proposed. Satellite DNA molecule (Y47β) was found to be associated with Y47 using the primers (beta01 and beta02) specific for DNAβ. Y47β consists of 1348 nucleotides, with a functional ORF (C1) in complementary-sense DNA. Y47β Has 62%-67% sequence identity with DNAβ molecule associated with Cotton leaf curl Multan virus or Cotton leaf curl Rajasthan virus, while lower than 46% sequence identities are found when compared with other reported DNAβ molecules. Relationship dendrograms show that DNAβ molecules are co-evolved with their help begomoviruses.

  17. Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer.

    Science.gov (United States)

    Chatterjee, Aparajita; Carpentieri, Andrea; Ratner, Daniel M; Bullitt, Esther; Costello, Catherine E; Robbins, Phillips W; Samuelson, John

    2010-08-19

    The infectious and diagnostic stage of Giardia lamblia (also known as G. intestinalis or G. duodenalis) is the cyst. The Giardia cyst wall contains fibrils of a unique beta-1,3-linked N-acetylgalactosamine (GalNAc) homopolymer and at least three cyst wall proteins (CWPs) composed of Leu-rich repeats (CWP(LRR)) and a C-terminal conserved Cys-rich region (CWP(CRR)). Our goals were to dissect the structure of the cyst wall and determine how it is disrupted during excystation. The intact Giardia cyst wall is thin (approximately 400 nm), easily fractured by sonication, and impermeable to small molecules. Curled fibrils of the GalNAc homopolymer are restricted to a narrow plane and are coated with linear arrays of oval-shaped protein complex. In contrast, cyst walls of Giardia treated with hot alkali to deproteinate fibrils of the GalNAc homopolymer are thick (approximately 1.2 microm), resistant to sonication, and permeable. The deproteinated GalNAc homopolymer, which forms a loose lattice of curled fibrils, is bound by native CWP1 and CWP2, as well as by maltose-binding protein (MBP)-fusions containing the full-length CWP1 or CWP1(LRR). In contrast, neither MBP alone nor MBP fused to CWP1(CRR) bind to the GalNAc homopolymer. Recombinant CWP1 binds to the GalNAc homopolymer within secretory vesicles of Giardia encysting in vitro. Fibrils of the GalNAc homopolymer are exposed during excystation or by treatment of heat-killed cysts with chymotrypsin, while deproteinated fibrils of the GalNAc homopolymer are degraded by extracts of Giardia cysts but not trophozoites. These results show the Leu-rich repeat domain of CWP1 is a lectin that binds to curled fibrils of the GalNAc homopolymer. During excystation, host and Giardia proteases appear to degrade bound CWPs, exposing fibrils of the GalNAc homopolymer that are digested by a stage-specific glycohydrolase.

  18. Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer.

    Directory of Open Access Journals (Sweden)

    Aparajita Chatterjee

    2010-08-01

    Full Text Available The infectious and diagnostic stage of Giardia lamblia (also known as G. intestinalis or G. duodenalis is the cyst. The Giardia cyst wall contains fibrils of a unique beta-1,3-linked N-acetylgalactosamine (GalNAc homopolymer and at least three cyst wall proteins (CWPs composed of Leu-rich repeats (CWP(LRR and a C-terminal conserved Cys-rich region (CWP(CRR. Our goals were to dissect the structure of the cyst wall and determine how it is disrupted during excystation. The intact Giardia cyst wall is thin (approximately 400 nm, easily fractured by sonication, and impermeable to small molecules. Curled fibrils of the GalNAc homopolymer are restricted to a narrow plane and are coated with linear arrays of oval-shaped protein complex. In contrast, cyst walls of Giardia treated with hot alkali to deproteinate fibrils of the GalNAc homopolymer are thick (approximately 1.2 microm, resistant to sonication, and permeable. The deproteinated GalNAc homopolymer, which forms a loose lattice of curled fibrils, is bound by native CWP1 and CWP2, as well as by maltose-binding protein (MBP-fusions containing the full-length CWP1 or CWP1(LRR. In contrast, neither MBP alone nor MBP fused to CWP1(CRR bind to the GalNAc homopolymer. Recombinant CWP1 binds to the GalNAc homopolymer within secretory vesicles of Giardia encysting in vitro. Fibrils of the GalNAc homopolymer are exposed during excystation or by treatment of heat-killed cysts with chymotrypsin, while deproteinated fibrils of the GalNAc homopolymer are degraded by extracts of Giardia cysts but not trophozoites. These results show the Leu-rich repeat domain of CWP1 is a lectin that binds to curled fibrils of the GalNAc homopolymer. During excystation, host and Giardia proteases appear to degrade bound CWPs, exposing fibrils of the GalNAc homopolymer that are digested by a stage-specific glycohydrolase.

  19. Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines.

    Science.gov (United States)

    Apostol, Lea Necitas G; Imagawa, Tomifumi; Suzuki, Akira; Masago, Yoshifumi; Lupisan, Socorro; Olveda, Remigio; Saito, Mariko; Omura, Tatsuo; Oshitani, Hitoshi

    2012-10-01

    Despite the vast distribution and expansive diversity of enteroviruses reported globally, indicators defining a complete view of the epidemiology of enteroviruses in tropical countries such as the Philippines are yet to be established. Detection of enteroviruses in the environment has been one of the markers of circulating viruses in a community. This study aimed to bridge the gap in the epidemiology of enteroviruses in the Philippines by providing an overview of the occurrence of enteroviruses in both urban and rural rivers. Molecular detection directed at the VP1 region of the enterovirus genome was performed on 44 grab river water samples collected from April to December 2009. The majority of the enterovirus serotypes detected were clustered with human enterovirus C species (HEV-C; 21/42), followed by HEV-B (12/42) and HEV-A (9/42). Porcine enterovirus 9 was also found in 12 out of 44 water samples. Phylogenetic analysis indicated that the viruses detected were closely related, if not all forming a monophyletic clade, with those enteroviruses detected previously from acute flaccid paralysis cases in the country. The clustering of environmental and human enterovirus strains implies that the circulation of these strains were associated with river contamination. This study gives further evidence of the environmental persistence of enteroviruses once they are shed in feces and likewise, provides additional data which may help in understanding the epidemiology of enteroviruses in humans, highlighting the need for more studies on the potential public health risks linked with enteroviruses found in the environment and their eventual clinical consequences in the country.

  20. Philippine Astronomy Convention 2009 Abstract: Program Offerings in Astronomy in the Philippines

    Science.gov (United States)

    Torres, J. R. F.

    2009-03-01

    The formal academic programs in Astronomy of the Rizal Technological University are the first such programs in the Philippines. The Master of Science in Astronomy program is envisioned to provide the student with a wide range of knowledge in many areas of Astronomy, leaning towards the descriptive aspects of knowledge. The student will choose the field or research most suitable to his or her interests. Three of these researches done while enrolled in the program, and even researches completed before the student actually enrolled in the program, may be considered as his or her thesis. The program suits professionals in all persuasions who wish to study Astronomy either for professional advancement or plainly for the love of the science or for intellectual satisfaction. Non-science majors can enroll. In 2008, the RTU Graduate School decided to ladderize the MS program and the Graduate Diploma in Astronomy was designed. This program is suited for science educators, astronomy lecturers and entrepreneurs, members of astronomical societies, and plain astronomy enthusiasts who like to gain in-depth knowledge in the most important aspects of astronomy. A bachelor's degree in any field is required. The program can be finished in two semesters and one summer. If the student opts to continue in the MS in Astronomy program, all the courses he or she has earned in the Diploma will be credited. The Bachelor of Science in Astronomy Technology is an intensive baccalaureate degree program designed to prepare students to become future research scientists and technologists in the field of Astronomy. The BS in Astronomy Technology is a cross-fertilized program, integrating interrelated sciences, such as engineering, geology, remote sensing, physics, atmospheric and environmental science, biology and biochemistry, and even philosophy and entrepreneurship into the study. Thus, the B.S. in Astronomy Technology program gives the student excellent job opportunities in many fields.

  1. Predation on the lizard Polychrus acutirostris (Squamata, Polychrotidae) by the curl-crested jay Cyanocorax cristatellus (Aves, Corvidae) in the Cerrado of Central Brazil

    OpenAIRE

    Frederico Gustavo Rodrigues França; Vívian da Silva Braz

    2009-01-01

    Predation on lizards is difficult to observe in nature. Here, we report for the first time an act of predation on the lizard Polychrus acutirostris by the Curl-crested Jay Cyanocorax cristatellus in a Cerrado area of the Chapada dos Veadeiros National Park, central Brazil, thus increasing knowledge of the diet of this bird species.

  2. Predation on the lizard Polychrus acutirostris (Squamata, Polychrotidae by the curl-crested jay Cyanocorax cristatellus (Aves, Corvidae in the Cerrado of Central Brazil

    Directory of Open Access Journals (Sweden)

    Frederico Gustavo Rodrigues França

    2009-09-01

    Full Text Available Predation on lizards is difficult to observe in nature. Here, we report for the first time an act of predation on the lizard Polychrus acutirostris by the Curl-crested Jay Cyanocorax cristatellus in a Cerrado area of the Chapada dos Veadeiros National Park, central Brazil, thus increasing knowledge of the diet of this bird species.

  3. Spatial and host-associated variation in prevalence and population density of wheat curl mite (Aceria tosichella) cryptic genotypes in agricultural landscapes.

    Science.gov (United States)

    The wheat curl mite (WCM), Aceria tosichella Keifer, is a major pest of cereals worldwide that also comprises a complex of at least 16 genetic lineages with divergent physiological traits, including host preference and specificity. The goal of this study was to test the extent to which host-plant sp...

  4. Excerpt from Dead Stars: American and Philippine Literary Perspectives on the American Colonization of the Philippines

    Directory of Open Access Journals (Sweden)

    Jennifer M. McMahon

    2011-12-01

    Full Text Available Dead Stars: American and Philippine Literary Perspectives on the American Colonization of the Philippines examines the American colonization of the Philippines from three distinct but related literary perspectives. The first is the reaction of anti-imperialist American writers Mark Twain, W. E. B. Du Bois, and William James to America’s first foray into the role of colonizer and how their varied essays, letters, and speeches provide an incisive delineation of fundamental conflicts in American identity at the turn of the twentieth century. The book then analyzes how these same conflicts surface in the colonial regime’s use of American literature as a tool to inculcate American values in the colonial educational system. Finally, Dead Stars considers the way three early and important Filipino writers—Paz Marquez Benitez, Maximo Kalaw, and Juan C. Laya—interpret and represent these same tensions in their fiction.

  5. Characteristics of virus and virus-like rose degeneration and dieback diseases

    Directory of Open Access Journals (Sweden)

    Marek S. Szyndel

    2013-12-01

    Full Text Available Presented review of rose diseases, associated with the plant degeneration symptoms, includes rose streak, rose colour break, rose rosette or rose witches' broom, rose dieback, rose wilt, rose stunt, rose leaf curl, rose spring dwarf, rose little leaf, rose "frisure", rose bud proliferation and rose flower proliferation. Most of these disorders are characterized by stunting or dwarfing of plants accompanied by a partial wilt and dieback of shoots. Proliferation and epinasty often occurred. Apart from detected viruses (TSV. SLRSV parts of degeneration syndrome have been transmitted by grafting other have failed to achieve any transmission of the causal agent. It seems likely that rose degeneration disorders in spite of symptom similarities are due to a complex of interacting factors including probably viruses.

  6. Clarification of the type locality of the Curl-crested Aracari Pteroglossus beauharnaesii Wagler, 1832 (Aves: Ramphastidae).

    Science.gov (United States)

    Costa, Thiago V V; Pacheco, José Fernando; Silveira, Luís Fábio

    2017-03-23

    The Curl-crested Aracari Pteroglossus beauharnaesii Wagler, 1832 is one of the most remarkable species in the family Ramphastidae. Among other distinctive plumage traits, its modified curly, shiny black crown feathers are unique in the family and are modified to an extent not found in any other living bird species (Brush 1967; Short & Horne 2002). The crown feathers, along with the distinct white throat, were considered sufficient justification in the past to include the species in the monotypic genus Beauharnaisius Bonaparte; however, molecular studies have shown that it is embedded within the genus Pteroglossus Illiger, sister to P. bitorquatus (Hackett & Lehn 1997; Eberhard & Bermingham 2005; Pereira & Wajntal 2008). It is a southern Amazonian species, occurring in the lowlands of northern Peru, north and central Bolivia and western and central Amazonian Brazil, south of the Marañon-Solimões-Amazonas rivers (Short & Horne 2002).

  7. Dual-Band Operation of a Circularly Polarized Four-Arm Curl Antenna with Asymmetric Arm Length

    Directory of Open Access Journals (Sweden)

    Son Xuat Ta

    2016-01-01

    Full Text Available This paper presents dual-band operation of a single-feed composite cavity-backed four-arm curl antenna. Dual-band operation is achieved with the presence of the asymmetrical arm structure. A pair of vacant-quarter printed rings is used in the feed structure to produce a good circular polarization (CP at both bands. The cavity-backed reflector is employed to improve the CP radiation characteristics in terms of the 3-dB axial ratio beamwidth and broadside gain. The proposed antenna is widely applicable in dual-band communication systems that have a small frequency ratio. Examples of such a system are global positioning systems.

  8. Rare Social Marketing for Sustainable Fishing in Cortes, Surigao Del Sur, Philippines

    Science.gov (United States)

    Day, Brian A.; DeWan, Amielle; Cadiz, Fel Ceasar; Jakosalem-Balane, Joy; Dueñas, Vincent; Trinidad, Pedro M., Jr.

    2014-01-01

    Rare's approach to promoting sustainable fishing through social marketing in the Philippines is exemplified in the Cortes Pride campaign. The Cortes Pride campaign is a social marketing behavior change program that was part of a cohort of 12 similar sustainable fishing campaigns in the Philippines, all of which used a unique blend of social…

  9. A Farewell to Welfare: Reflections on the Political Function of Social Welfare in the Philippines

    NARCIS (Netherlands)

    J.B.W. Kuitenbrouwer (Joost)

    1979-01-01

    textabstractThis analysis is the result of a study undertaken in the Philippines in the course of 1975. Fieldwork was preceded and followed by a series of meetings with officials of the Department of Social Welfare and of other Departments, as well as with scholars of the University of the Philippin

  10. Getting a Grip on Climate Change in the Philippines : Extended Technical Report

    OpenAIRE

    World Bank

    2013-01-01

    Philippines currently experience and will continue to face significant impacts from climate change. To ensure climate resilience, build a low-carbon economy, and increase its role in the global climate change dialogue, the Philippine government has launched strong climate policy and institutional and financing reforms, supported by a clear rationale for no-regrets action. However, transfor...

  11. Getting a Grip on Climate Change in the Philippines : Executive Report

    OpenAIRE

    World Bank

    2013-01-01

    The Philippines already experiences and will continue to face impacts from climate change. In the decades ahead, the most serious consequences will be felt in coastal and urban areas. Severe hardships are expected in agriculture and fisheries, leading to negative impacts on jobs and the economy. With these risks in mind the Philippine Government has initiated significant climate reforms, e...

  12. Collaborative Philippine-Canadian Action Cycles for Strategic International Coastal Ecohealth

    Science.gov (United States)

    Watts, Paul David; Pajaro, Marivic Gosamo

    2014-01-01

    Canadian-Philippine linkages on multi-year coastal Action Research and learning cycles are detailed within established participatory development strategies. Philippine sustainable development is further considered as a function of inter-jurisdictional considerations, and reflexive role shifts for academe. An organizational process is outlined to…

  13. Rare Social Marketing for Sustainable Fishing in Cortes, Surigao Del Sur, Philippines

    Science.gov (United States)

    Day, Brian A.; DeWan, Amielle; Cadiz, Fel Ceasar; Jakosalem-Balane, Joy; Dueñas, Vincent; Trinidad, Pedro M., Jr.

    2014-01-01

    Rare's approach to promoting sustainable fishing through social marketing in the Philippines is exemplified in the Cortes Pride campaign. The Cortes Pride campaign is a social marketing behavior change program that was part of a cohort of 12 similar sustainable fishing campaigns in the Philippines, all of which used a unique blend of social…

  14. Philippine Fig wasps 1. Records and descriptions of Otitesellini (Hymenoptera Chalcidoidea, Torymidae)

    NARCIS (Netherlands)

    Wiebes, J.T.

    1974-01-01

    In 1964, by awarding to me that year's proceeds of the "Pieter Langerhuizen Fonds", the Hollandsche Maatschappij der Wetenschappen enabled me to study figs and fig wasps in the Philippines. While several Philippine fig wasps are already known from the papers by Ashmead (1904, 1905), Brown (1906), Ba

  15. Statistics Education Research in Malaysia and the Philippines: A Comparative Analysis

    Science.gov (United States)

    Reston, Enriqueta; Krishnan, Saras; Idris, Noraini

    2014-01-01

    This paper presents a comparative analysis of statistics education research in Malaysia and the Philippines by modes of dissemination, research areas, and trends. An electronic search for published research papers in the area of statistics education from 2000-2012 yielded 20 for Malaysia and 19 for the Philippines. Analysis of these papers showed…

  16. A Tale of Two Countries: Comparing Civic Education in the Philippines and Singapore

    Science.gov (United States)

    Baildon, Mark; Sim, Jasmine B.-Y.; Paculdar, Agnes

    2016-01-01

    This article provides a comparative analysis of citizenship education in the Philippines and Singapore. Through an analysis of historical contexts, citizenship education policy and curriculum, it examines "Makabayan" in the Philippines and "National Education" in Singapore. It identifies particular policy and curriculum…

  17. Arguing in L2: Discourse Structure and Textual Metadiscourse in Philippine Newspaper Editorials

    Science.gov (United States)

    Tarrayo, Veronico N.; Duque, Marie Claire T.

    2011-01-01

    This study described the discourse structure and textual metadiscourse in newspaper editorials in the Philippines where English is used as a second language or L2. Specifically, it sought answers to the following questions: (1) What discourse features characterize the structure of the following parts of Philippine newspaper editorials--orientation…

  18. Rare Social Marketing for Sustainable Fishing in Cortes, Surigao Del Sur, Philippines

    Science.gov (United States)

    Day, Brian A.; DeWan, Amielle; Cadiz, Fel Ceasar; Jakosalem-Balane, Joy; Dueñas, Vincent; Trinidad, Pedro M., Jr.

    2014-01-01

    Rare's approach to promoting sustainable fishing through social marketing in the Philippines is exemplified in the Cortes Pride campaign. The Cortes Pride campaign is a social marketing behavior change program that was part of a cohort of 12 similar sustainable fishing campaigns in the Philippines, all of which used a unique blend of social…

  19. 77 FR 31829 - Importation of Fresh Bananas From the Philippines Into the Continental United States...

    Science.gov (United States)

    2012-05-30

    ... the Continental United States'' and published in the Federal Register on April 16, 2012 (77 FR 22510... Animal and Plant Health Inspection Service Importation of Fresh Bananas From the Philippines Into the... the importation of fresh bananas from the Philippines into the continental United States....

  20. An Assessment of the Armed Forces of the Philippines Modernization Program

    Science.gov (United States)

    2007-12-14

    consultations, exchanges, and the like ( Narcise 2003, 6). Although the VFA would not entail the reopening of the US bases in the country, the signing...AFP acquisition system. Commandant’s Paper, Armed Forces of the Philippines Command and General Staff College, Quezon City, Philippines. Narcise