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  1. Mixed cultures of Kimchi lactic acid bacteria show increased cell ...

    African Journals Online (AJOL)

    ufuoma

    kimchi lactobacilli on batch fermentation increased the cell density and lactic acid production with low nutrients .... first series of fermentations were carried out using pure cultures of each ... Each number represents the mean. ± SD of three ...

  2. Higher frequency of social learning in China than in the West shows cultural variation in the dynamics of cultural evolution.

    Science.gov (United States)

    Mesoudi, Alex; Chang, Lei; Murray, Keelin; Lu, Hui Jing

    2015-01-07

    Cultural evolutionary models have identified a range of conditions under which social learning (copying others) is predicted to be adaptive relative to asocial learning (learning on one's own), particularly in humans where socially learned information can accumulate over successive generations. However, cultural evolution and behavioural economics experiments have consistently shown apparently maladaptive under-utilization of social information in Western populations. Here we provide experimental evidence of cultural variation in people's use of social learning, potentially explaining this mismatch. People in mainland China showed significantly more social learning than British people in an artefact-design task designed to assess the adaptiveness of social information use. People in Hong Kong, and Chinese immigrants in the UK, resembled British people in their social information use, suggesting a recent shift in these groups from social to asocial learning due to exposure to Western culture. Finally, Chinese mainland participants responded less than other participants to increased environmental change within the task. Our results suggest that learning strategies in humans are culturally variable and not genetically fixed, necessitating the study of the 'social learning of social learning strategies' whereby the dynamics of cultural evolution are responsive to social processes, such as migration, education and globalization.

  3. Challenging patient safety culture: survey results.

    Science.gov (United States)

    Hellings, Johan; Schrooten, Ward; Klazinga, Niek; Vleugels, Arthur

    2007-01-01

    The purpose of this paper is to measure patient safety culture in five Belgian general hospitals. Safety culture plays an important role in the approach towards greater patient safety in hospitals. The Patient Safety Culture Hospital questionnaire was distributed hospital-wide in five general hospitals. It evaluates ten patient safety culture dimensions and two outcomes. The scores were expressed as the percentage of positive answers towards patient safety for each dimension. The survey was conducted from March through November 2005. In total, 3,940 individuals responded (overall response rate = 77 per cent), including 2,813 nurses and assistants, 462 physicians, 397 physiotherapists, laboratory and radiology assistants, social workers and 64 pharmacists and pharmacy assistants. The dimensional positive scores were found to be low to average in all the hospitals. The lowest scores were "hospital management support for patient safety" (35 per cent), "non-punitive response to error" (36 per cent), "hospital transfers and transitions" (36 per cent), "staffing" (38 per cent), and "teamwork across hospital units" (40 per cent). The dimension "teamwork within hospital units" generated the highest score (70 per cent). Although the same dimensions were considered problematic in the different hospitals, important variations between the five hospitals were observed. A comprehensive and tailor-made plan to improve patient safety culture in these hospitals can now be developed. Results indicate that important aspects of the patient safety culture in these hospitals need improvement. This is an important challenge to all stakeholders wishing to improve patient safety.

  4. THE "GREATEST SHOW ON EARTH": FOOTBALL AND ITS ABILITY TO BREAK THE LEVELS OF MASS CULTURE

    OpenAIRE

    Humberto Ivan Keske; Cleber Cristiano Prodanov; Vinícius Moser

    2012-01-01

    This article examines how the media, especially radio, television and web, strengthen football and give national and international visibility to this sport, within the different levels of culture proposed by Umberto Eco (2004). The text also shows how soccer provides a contextual and dialogical relationship between the different levels of culture, being able to transgress them, rearranging them into a new proposal.

  5. The Construction of National Memory Through Popular Culture. The Case of the Argentinian TV Show,

    Directory of Open Access Journals (Sweden)

    Elizondo Oviedo, María Verónica

    2010-07-01

    Full Text Available This article addresses the borders of the complex television sign through the analysis of the Argentinian TV show "Peter Capusotto y sus videos". In it we find that humor is used as a mechanism to dismantle binary hierarchic political and social oppositions. The program is presented as a musical and comedy TV show where concepts such as popular culture and mass culture are questioned through different false musicians. We focus our attention in the character of Bombita Rodríguez, el Palito Ortega montonero due to the controversy generated in the cultural scene by the fact of bringing up recent aspects of Argentinian history.

  6. THE "GREATEST SHOW ON EARTH": FOOTBALL AND ITS ABILITY TO BREAK THE LEVELS OF MASS CULTURE

    Directory of Open Access Journals (Sweden)

    Humberto Ivan Keske

    2012-07-01

    Full Text Available This article examines how the media, especially radio, television and web, strengthen football and give national and international visibility to this sport, within the different levels of culture proposed by Umberto Eco (2004. The text also shows how soccer provides a contextual and dialogical relationship between the different levels of culture, being able to transgress them, rearranging them into a new proposal.

  7. Cultured corneas show dendritic spread and restrict herpes simplex virus infection that is not observed with cultured corneal cells

    Science.gov (United States)

    Thakkar, Neel; Jaishankar, Dinesh; Agelidis, Alex; Yadavalli, Tejabhiram; Mangano, Kyle; Patel, Shrey; Tekin, Sati Zeynep; Shukla, Deepak

    2017-01-01

    Herpes simplex virus-1 (HSV-1) causes life-long morbidities in humans. While fever blisters are more common, occasionally the cornea is infected resulting in vision loss. A very intriguing aspect of HSV-1 corneal infection is that the virus spread is normally restricted to only a small fraction of cells on the corneal surface that connect with each other in a dendritic fashion. Here, to develop a comprehensive understanding of the susceptibility of human corneal epithelial (HCE) cells to HSV-1 infection, we infected HCE cells at three different dosages of HSV-1 and measured the outcomes in terms of viral entry, gene and protein expression, viral replication and cytokine induction. In cultured cells, infectivity and cytokine induction were observed even at the minimum viral dosage tested, while a more pronounced dose-restricted infectivity was seen in ex vivo cultures of porcine corneas. Use of fluorescent HSV-1 virions demonstrated a pattern of viral spread ex vivo that mimics clinical findings. We conclude that HCE cell cultures are highly susceptible to infection whereas the cultured corneas demonstrate a higher ability to restrict the infection even in the absence of systemic immune system. The restriction is helped in part by local interferon response and the unique cellular architecture of the cornea. PMID:28198435

  8. Culturable associated-bacteria of the sponge Theonella swinhoei show tolerance to high arsenic concentrations

    Directory of Open Access Journals (Sweden)

    Ray eKeren

    2015-02-01

    Full Text Available Sponges are potent filter feeders and as such are exposed to high fluxes of toxic trace elements, which can accumulate in their body over time. Such is the case of the Red Sea sponge Theonella swinhoei, which has been shown to accumulate up to 8500 mg/Kg of the highly toxic element arsenic. T. swinhoei is known to harbor a multitude of sponge-associated bacteria, so it is hypothesized that the associated-bacteria will be tolerant to high arsenic concentration. This study also investigates the fate of the arsenic accumulated in the sponge to test if the associated-bacteria have an important role in the arsenic accumulation process of their host, since bacteria are key players in the natural arsenic cycle. Separation of the sponge to sponge cells and bacteria enriched fractions showed that arsenic is accumulated by the bacteria. Sponge-associated, arsenic-tolerant bacteria were cultured in the presence of 5 mM of either arsenate or arsenite (equivalent to 6150 mg/Kg arsenic, dry weight. The 54 isolated bacteria were grouped to 15 OTUs and isolates belonging to 12 OTUs were assessed for tolerance to arsenate at increased concentrations up to 100 mM. Eight of the 12 OTUs tolerated an order of magnitude increase in the concentration of arsenate, and some exhibited external biomineralization of arsenic-magnesium salts. The biomineralization of this unique mineral was directly observed in bacteria for the first time. These results may provide an explanation for the ability of the sponge to accumulate considerable amounts of arsenic. Furthermore arsenic-mineralizing bacteria can potentially be used for the study of bioremediation, as arsenic toxicity affects millions of people worldwide.

  9. Amnion-derived mesenchymal stromal cells show a mesenchymal-epithelial phenotype in culture.

    Science.gov (United States)

    König, Julia; Lang, Ingrid; Siwetz, Monika; Fröhlich, Julia; Huppertz, Berthold

    2014-06-01

    The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to change their phenotype in culture. We analyzed the mesenchymal phenotype of hAMSC at different stages after isolation using immunohistochemistry. Shortly after isolation (1 day), 92 % (± 7 %) of the hAMSC expressed the mesenchymal marker vimentin, 2 % (± 1 %) stained for the epithelial marker cytokeratin-7 and 5 % (± 4 %) co-expressed these markers. After 5 days, the double positive cells slightly increased to 7 % (± 3 %), while exclusive expression of cytokeratin-7 or vimentin remained unchanged (1 % ± 2 % and 92 % ± 1 %, respectively). After the first passage, all attached cells were vimentin-positive, while 54 % (± 9 %) co-expressed cytokeratin-7 and vimentin. Thus, we conclude that under culture, hAMSC adopt a hybrid mesenchymal-epithelial phenotype. It is also essential to perform microscopical examination during the first days after isolation to detect contaminations with human amnion-derived epithelial cells in cultures of hAMSC.

  10. Improve Results of English Teaching Through Fostering Students' Cultural Competence

    Institute of Scientific and Technical Information of China (English)

    郭秀梅

    2004-01-01

    This paper, based on recent overseas and home research findings and the author's teaching experiences,discusses the close relationship between culture background knowledge and English teaching, analyses the possible reasons for students' deficiency of culture background Knowledge, especially proposes several practical approaches of English teaching to help English teachers develop students' culture background knowledge so as to foster students' cultural competence and then improve the results of English teaching.

  11. Crustacean peptidergic neurons in culture show immediate outgrowth in simple medium.

    Science.gov (United States)

    Cooke, I; Graf, R; Grau, S; Haylett, B; Meyers, D; Ruben, P

    1989-01-01

    The survival and outgrowth of neurons in culture has usually required conditioning factors. We now report that crustacean neurons, taken from the peptidergic neurosecretory system of the eyestalk of crabs (Cardisoma carnifex) and lobsters (Panulirus marginatus), show immediate outgrowth, sustained for a week or more, in defined medium as simple as physiological saline with glucose and glutamine. The neurons show peptide hormone immunoreactivity that is prominent at growth cones, exhibit differences in form correlated with their immunoreactivity, release peptides to the medium, and have voltage-dependent currents, including a well-sustained Ca current. Cd blocks secretion, growth, and the Ca current. Peptidergic secretory neurons may be able to utilize existing membrane from their store of granules and already active synthetic, transport, and secretory mechanisms for immediate outgrowth.

  12. The dietary biogenic amines tyramine and histamine show synergistic toxicity towards intestinal cells in culture.

    Science.gov (United States)

    Del Rio, Beatriz; Redruello, Begoña; Linares, Daniel M; Ladero, Victor; Fernandez, Maria; Martin, Maria Cruz; Ruas-Madiedo, Patricia; Alvarez, Miguel A

    2017-03-01

    Tyramine and histamine are the biogenic amines (BA) most commonly found at high concentrations in food; they may even appear together at toxic concentrations. The present work examines, via real-time cell analysis, whether histamine and tyramine show synergistic toxicity towards intestinal cell cultures. Employing a constant equipotency ratio, their interaction was examined via the combination index (CI) method of Chou & Talalay. Co-treatment with tyramine and histamine was associated with a stronger cytotoxic effect than was treatment with either BA or on its own. Indeed, a synergistic interaction (CIhistamine, at concentrations below the legal limit, increases the cytotoxicity of tyramine at concentrations frequently reached in some foods. The synergistic cytotoxicity of tyramine and histamine should be taken into account when establishing legal limits designed to ensure consumer safety.

  13. Cultural consultation as a model for training multidisciplinary mental healthcare professionals in cultural competence skills: preliminary results.

    Science.gov (United States)

    Owiti, J A; Ajaz, A; Ascoli, M; de Jongh, B; Palinski, A; Bhui, K S

    2014-01-01

    Lack of cultural competence in care contributes to poor experiences and outcomes from care for migrants and racial and ethnic minorities. As a result, health and social care organizations currently promote cultural competence of their workforce as a means of addressing persistent poor experiences and outcomes. At present, there are unsystematic and diverse ways of promoting cultural competence, and their impact on clinician skills and patient outcomes is unknown. We developed and implemented an innovative model, cultural consultation service (CCS), to promote cultural competence of clinicians and directly improve on patient experiences and outcomes from care. CCS model is an adaptation of the McGill model, which uses ethnographic methodology and medical anthropological knowledge. The method and approach not only contributes both to a broader conceptual and dynamic understanding of culture, but also to learning of cultural competence skills by healthcare professionals. The CCS model demonstrates that multidisciplinary workforce can acquire cultural competence skills better through the clinical encounter, as this promotes integration of learning into day-to-day practice. Results indicate that clinicians developed a broader and patient-centred understanding of culture, and gained skills in narrative-based assessment method, management of complexity of care, competing assumptions and expectations, and clinical cultural formulation. Cultural competence is defined as a set of skills, attitudes and practices that enable the healthcare professionals to deliver high-quality interventions to patients from diverse cultural backgrounds. Improving on the cultural competence skills of the workforce has been promoted as a way of reducing ethnic and racial inequalities in service outcomes. Currently, diverse models for training in cultural competence exist, mostly with no evidence of effect. We established an innovative narrative-based cultural consultation service in an inner

  14. School Nurse Cultural Competence Needs Assessment: Results and Response.

    Science.gov (United States)

    Matza, Maria; Maughan, Erin; Barrows, Beth M

    2015-11-01

    NASN conducted a needs assessment to learn about the cultural competence skills needed by school nurses. The purpose of this article is to describe the results of the needs assessment and describe actions taken to address cultural competency needs for the U.S. school nurse workforce.

  15. Astronomy Diagnostic Test Results Reflect Course Goals and Show Room for Improvement

    Science.gov (United States)

    LoPresto, Michael C.

    2007-01-01

    The results of administering the Astronomy Diagnostic Test (ADT) to introductory astronomy students at Henry Ford Community College over three years have shown gains comparable with national averages. Results have also accurately corresponded to course goals, showing greater gains in topics covered in more detail, and lower gains in topics covered…

  16. Gun Shows and Gun Violence: Fatally Flawed Study Yields Misleading Results

    Science.gov (United States)

    Hemenway, David; Webster, Daniel; Pierce, Glenn; Braga, Anthony A.

    2010-01-01

    A widely publicized but unpublished study of the relationship between gun shows and gun violence is being cited in debates about the regulation of gun shows and gun commerce. We believe the study is fatally flawed. A working paper entitled “The Effect of Gun Shows on Gun-Related Deaths: Evidence from California and Texas” outlined this study, which found no association between gun shows and gun-related deaths. We believe the study reflects a limited understanding of gun shows and gun markets and is not statistically powered to detect even an implausibly large effect of gun shows on gun violence. In addition, the research contains serious ascertainment and classification errors, produces results that are sensitive to minor specification changes in key variables and in some cases have no face validity, and is contradicted by 1 of its own authors’ prior research. The study should not be used as evidence in formulating gun policy. PMID:20724672

  17. Gun shows and gun violence: fatally flawed study yields misleading results.

    Science.gov (United States)

    Wintemute, Garen J; Hemenway, David; Webster, Daniel; Pierce, Glenn; Braga, Anthony A

    2010-10-01

    A widely publicized but unpublished study of the relationship between gun shows and gun violence is being cited in debates about the regulation of gun shows and gun commerce. We believe the study is fatally flawed. A working paper entitled "The Effect of Gun Shows on Gun-Related Deaths: Evidence from California and Texas" outlined this study, which found no association between gun shows and gun-related deaths. We believe the study reflects a limited understanding of gun shows and gun markets and is not statistically powered to detect even an implausibly large effect of gun shows on gun violence. In addition, the research contains serious ascertainment and classification errors, produces results that are sensitive to minor specification changes in key variables and in some cases have no face validity, and is contradicted by 1 of its own authors' prior research. The study should not be used as evidence in formulating gun policy.

  18. The Visual Cultures of Showing ‘Nourishment’ in a Glass Case

    DEFF Research Database (Denmark)

    Buhl, Mie

    2016-01-01

    by placing it where you cannot touch it, eat it, smell it, taste it – in other words of turning it into an exhibition item? By removing the characteristics from food, it would in all probability be even more difficult to attain the purpose of exhibiting it: to sell it to students and staff. My discussion...... of the phenomenon of nourishment in glass cases draws on approaches developed and discussed as visual culture methodology. With a visual culture approach, visuals work as constructions of visual knowledge and as indicators of the relation between an observer's glance and the phenomenon observed. The implication...

  19. Human Airway Primary Epithelial Cells Show Distinct Architectures on Membrane Supports Under Different Culture Conditions.

    Science.gov (United States)

    Min, Kyoung Ah; Rosania, Gus R; Shin, Meong Cheol

    2016-06-01

    To facilitate drug development for lung delivery, it is highly demanding to establish appropriate airway epithelial cell models as transport barriers to evaluate pharmacokinetic profiles of drug molecules. Besides the cancer-derived cell lines, as the primary cell model, normal human bronchial epithelial (NHBE) cells have been used for drug screenings because of physiological relevance to in vivo. Therefore, to accurately interpret drug transport data in NHBE measured by different laboratories, it is important to know biophysical characteristics of NHBE grown on membranes in different culture conditions. In this study, NHBE was grown on the polyester membrane in a different medium and its transport barrier properties as well as cell architectures were fully characterized by functional assays and confocal imaging throughout the days of cultures. Moreover, NHBE cells on inserts in a different medium were subject to either of air-interfaced culture (AIC) or liquid-covered culture (LCC) condition. Cells in the AIC condition were cultivated on the membrane with medium in the basolateral side only, whereas cells with medium in apical and basolateral sides under the LCC condition. Quantitative microscopic imaging with biophysical examination revealed distinct multilayered architectures of differentiated NHBE cells, suggesting NHBE as functional cell barriers for the lung-targeting drug transport.

  20. Feasibility Study on the Development of Index that Shows Social and Cultural Acceptance of Nuclear Power

    Energy Technology Data Exchange (ETDEWEB)

    Cho, SeongKyung [Bangmok College of General Education at Myongji Univ., Yongin (Korea, Republic of); Choi, Seungho; Yoon, Hana; Song, Jiyeon [Domo Brodeur, Seoul (Korea, Republic of)

    2015-05-15

    In this context, it is necessary to manage and develop an index that can measure the level of public acceptance by establishing the terms of social/cultural public acceptance of nuclear power in a practical manner and by identifying influential factors of public acceptance. Developing an index itself is not intended to increase the public acceptance of nuclear power. This study intends to contribute to determining energy policy acceptable to the public by estimating the level of potential social conflicts related to nuclear power policies with eligible evaluation criteria on social/cultural acceptance and by reducing relevant social costs. Key conclusions and proposal of this research are as follows. First, the influential factors of acceptance are reliability of nuclear safety, risk perception of nuclear power and beneficial perception of nuclear power. Among them, reliability of nuclear safety appears to have the most influence. In addition, benefit perception of nuclear power at the social level is significantly higher than that at the individual level. However, in relation to risk perception, a gap between experts and the public is found as nuclear industry premises that accident does not occur while the public premises that accident may occur.

  1. Bacteriologic results from 4,000 root canal cultures

    NARCIS (Netherlands)

    Winkler, K.C.; Amerongen, J. van

    1959-01-01

    The bacteriologic results from more than 4,000 root canal cultures are discussed. Streptococci form 61 per cent of the isolated organisms. Among these, the group of hemolytic, indifferent, and indifferent anaerobic streptococci, mainly belonging to serologic groups F, G, and C, seem the most serious

  2. Nanotextured polymer substrates show enhanced cancer cell isolation and cell culture

    Science.gov (United States)

    Islam, Muhymin; Sajid, Adeel; Arif Iftakher Mahmood, M.; Motasim Bellah, Mohammad; Allen, Peter B.; Kim, Young-Tae; Iqbal, Samir M.

    2015-06-01

    Detection of circulating tumor cells (CTCs) in the early stages of cancer is a great challenge because of their exceedingly small concentration. There are only a few approaches sensitive enough to differentiate tumor cells from the plethora of other cells in a sample like blood. In order to detect CTCs, several antibodies and aptamers have already shown high affinity. Nanotexture can be used to mimic basement membrane to further enhance this affinity. This article reports an approach to fabricate nanotextured polydimethylsiloxane (PDMS) substrates using micro reactive ion etching (micro-RIE). Three recipes were used to prepare nanotextured PDMS using oxygen and carbon tetrafluoride. Micro-RIE provided better control on surface properties. Nanotexturing improved the affinity of PDMS surfaces to capture cancer cells using surface immobilized aptamers against cell membrane overexpressed with epidermal growth factor receptors. In all cases, nanotexture of PDMS increased the effective surface area by creating nanoscale roughness on the surface. Nanotexture also enhanced the growth rate of cultured cells compared to plain surfaces. A comparison among the three nanotextured surfaces demonstrated an almost linear relationship between the surface roughness and density of captured tumor cells. The nanotextured PDMS mimicked biophysical environments for cells to grow faster. This can have many implications in microfluidic platforms used for cell handling.

  3. Chaud show et froid à Libreville : consommation alimentaire et culture urbaine

    OpenAIRE

    Pourtier, R.

    1998-01-01

    Technological advances in food conservation have led to radical changes in eating habits in the capital of Gabon. A novel baking technique using microwave ovens has led to a revolution in bread production and distribution. As of 1987, fifty stands called "Hot Shows" (from the French term "Chaud Show") mushroomed throughout the city, selling crisp baguettes and pastries. Along with the more traditional-style bakeries, they have become new areas of urban life. Futhermore, the large proportion o...

  4. Pilot evaluation of a media literacy program for tobacco prevention targeting early adolescents shows mixed results.

    Science.gov (United States)

    Kaestle, Christine E; Chen, Yvonnes; Estabrooks, Paul A; Zoellner, Jamie; Bigby, Brandon

    2013-01-01

    The purpose of this pilot study was to assess the impact of media literacy for tobacco prevention for youth delivered through a community site. A randomized pretest-posttest evaluation design with matched-contact treatment and control conditions. The pilot study was delivered through the YMCA in a lower-income suburban and rural area of Southwest Virginia, a region long tied, both economically and culturally, to the tobacco industry. Children ages 8 to 14 (76% white, 58% female) participated in the study (n = 38). The intervention was an antismoking media literacy program (five 1-hour lessons) compared with a matched-contact creative writing control program. General media literacy, three domains of tobacco-specific media literacy ("authors and audiences," "messages and meanings," and "representation and reality"), tobacco attitudes, and future expectations were assessed. Multiple regression modeling assessed the impact of the intervention, controlling for pretest measures, age, and sex. General media literacy and tobacco-specific "authors and audiences" media literacy improved significantly for treatment compared with control (p media literacy measures and for tobacco attitudes were not significant. Future expectations of smoking increased significantly for treatment participants ages 10 and younger (p media literacy are accompanied by an increase in future expectations to smoke for younger children.

  5. Long-Term Trial Results Show No Mortality Benefit from Annual Prostate Cancer Screening

    Science.gov (United States)

    Thirteen year follow-up data from the Prostate, Lung, Colorectal and Ovarian (PLCO) cancer screening trial show higher incidence but similar mortality among men screened annually with the prostate-specific antigen (PSA) test and digital rectal examination

  6. Comparison of some results of program SHOW with other solar hot water computer programs

    Science.gov (United States)

    Young, M. F.; Baughn, J. W.

    Subroutines and the driver program for the simulation code SHOW (solar hot water) for solar thermosyphon systems are discussed, and simulations are compared with predictions by the F-CHART and TRNSYS codes. SHOW has the driver program MAIN, which defines the system control logic for choosing the appropriate system subroutine for analysis. Ten subroutines are described, which account for the solar system physical parameters, the weather data, the manufacturer-supplied system specifications, mass flow rates, pumped systems, total transformed radiation, load use profiles, stratification in storage, an electric water heater, and economic analyses. The three programs are employed to analyze a thermosiphon installation in Sacramento with two storage tanks. TRNSYS and SHOW were in agreement and lower than F-CHARt for annual predictions, although significantly more computer time was necessary to make TRNSYS converge.

  7. Results From Mars Show Electrostatic Charging of the Mars Pathfinder Sojourner Rover

    Science.gov (United States)

    Kolecki, Joseph C.; Siebert, Mark W.

    1998-01-01

    Indirect evidence (dust accumulation) has been obtained indicating that the Mars Pathfinder rover, Sojourner, experienced electrostatic charging on Mars. Lander camera images of the Sojourner rover provide distinctive evidence of dust accumulation on rover wheels during traverses, turns, and crabbing maneuvers. The sol 22 (22nd Martian "day" after Pathfinder landed) end-of-day image clearly shows fine red dust concentrated around the wheel edges with additional accumulation in the wheel hubs. A sol 41 image of the rover near the rock "Wedge" (see the next image) shows a more uniform coating of dust on the wheel drive surfaces with accumulation in the hubs similar to that in the previous image. In the sol 41 image, note particularly the loss of black-white contrast on the Wheel Abrasion Experiment strips (center wheel). This loss of contrast was also seen when dust accumulated on test wheels in the laboratory. We believe that this accumulation occurred because the Martian surface dust consists of clay-sized particles, similar to those detected by Viking, which have become electrically charged. By adhering to the wheels, the charged dust carries a net nonzero charge to the rover, raising its electrical potential relative to its surroundings. Similar charging behavior was routinely observed in an experimental facility at the NASA Lewis Research Center, where a Sojourner wheel was driven in a simulated Martian surface environment. There, as the wheel moved and accumulated dust (see the following image), electrical potentials in excess of 100 V (relative to the chamber ground) were detected by a capacitively coupled electrostatic probe located 4 mm from the wheel surface. The measured wheel capacitance was approximately 80 picofarads (pF), and the calculated charge, 8 x 10(exp -9) coulombs (C). Voltage differences of 100 V and greater are believed sufficient to produce Paschen electrical discharge in the Martian atmosphere. With an accumulated net charge of 8 x 10(exp

  8. Data for behavioral results and brain regions showing a time effect during pair-association retrieval

    Directory of Open Access Journals (Sweden)

    Koji Jimura

    2016-09-01

    Full Text Available The current data article provides behavioral and neuroimaging data for the research article "Relatedness-dependent rapid development of brain activity in anterior temporal cortex during pair-association retrieval” (Jimura et al., 2016 [1]. Behavioral performance is provided in a table. Fig. 2 of the article is based on this table. Brain regions showing time effect are provided in a table. A statistical activation map for the time effect is shown in Fig. 3C of the article.

  9. A cross-cultural comparison of high school students' responses to a science centre show on the physics of sound in South Africa.

    Science.gov (United States)

    Fish, Derek; Allie, Saalih; Pelaez, Nancy; Anderson, Trevor

    2016-04-25

    We report on the attitudes and ideas developed by students from three distinct school groups to a science show about sound. We addressed two research questions: (1) How do the students compare with respect to their (a) attitudes to the sound show and to science in general and (b) changes in conceptual understanding as a result of the show and (2) what changes could be made to the show, and to science shows in general, that would be sensitive to the cultural and language differences of the groups? These were addressed by multiple-choice, pre- and post-tests comprising both attitudinal and conceptual questions. Our results pointed to a common enjoyment of the show but a different understanding of concepts and consequent learning, which suggest that science shows (and science teaching) need to be adjusted to accommodate different cultural groups for maximum impact.

  10. Animation shows promise in initiating timely cardiopulmonary resuscitation: results of a pilot study.

    Science.gov (United States)

    Attin, Mina; Winslow, Katheryn; Smith, Tyler

    2014-04-01

    Delayed responses during cardiac arrest are common. Timely interventions during cardiac arrest have a direct impact on patient survival. Integration of technology in nursing education is crucial to enhance teaching effectiveness. The goal of this study was to investigate the effect of animation on nursing students' response time to cardiac arrest, including initiation of timely chest compression. Nursing students were randomized into experimental and control groups prior to practicing in a high-fidelity simulation laboratory. The experimental group was educated, by discussion and animation, about the importance of starting cardiopulmonary resuscitation upon recognizing an unresponsive patient. Afterward, a discussion session allowed students in the experimental group to gain more in-depth knowledge about the most recent changes in the cardiac resuscitation guidelines from the American Heart Association. A linear mixed model was run to investigate differences in time of response between the experimental and control groups while controlling for differences in those with additional degrees, prior code experience, and basic life support certification. The experimental group had a faster response time compared with the control group and initiated timely cardiopulmonary resuscitation upon recognition of deteriorating conditions (P < .0001). The results demonstrated the efficacy of combined teaching modalities for timely cardiopulmonary resuscitation. Providing opportunities for repetitious practice when a patient's condition is deteriorating is crucial for teaching safe practice.

  11. FES Training in Aging: interim results show statistically significant improvements in mobility and muscle fiber size

    Directory of Open Access Journals (Sweden)

    Helmut Kern

    2012-03-01

    Full Text Available Aging is a multifactorial process that is characterized by decline in muscle mass and performance. Several factors, including reduced exercise, poor nutrition and modified hormonal metabolism, are responsible for changes in the rates of protein synthesis and degradation that drive skeletal muscle mass reduction with a consequent decline of force generation and mobility functional performances. Seniors with normal life style were enrolled: two groups in Vienna (n=32 and two groups in Bratislava: (n=19. All subjects were healthy and declared not to have any specific physical/disease problems. The two Vienna groups of seniors exercised for 10 weeks with two different types of training (leg press at the hospital or home-based functional electrical stimulation, h-b FES. Demografic data (age, height and weight were recorded before and after the training period and before and after the training period the patients were submitted to mobility functional analyses and muscle biopsies. The mobility functional analyses were: 1. gait speed (10m test fastest speed, in m/s; 2. time which the subject needed to rise from a chair for five times (5x Chair-Rise, in s; 3. Timed –Up-Go- Test, in s; 4. Stair-Test, in s; 5. isometric measurement of quadriceps force (Torque/kg, in Nm/kg; and 6. Dynamic Balance in mm. Preliminary analyses of muscle biopsies from quadriceps in some of the Vienna and Bratislava patients present morphometric results consistent with their functional behaviors. The statistically significant improvements in functional testings here reported demonstrates the effectiveness of h-b FES, and strongly support h-b FES, as a safe home-based method to improve contractility and performances of ageing muscles.

  12. Reception of Talent Shows in Denmark: First Results from a Trans-National Audience Study of a Global Format Genre

    DEFF Research Database (Denmark)

    Jensen, Pia Majbritt

    This paper will discuss the methodology and present the preliminary findings of the Danish part of a trans-national, comparative audience study of the musical talent show genre undertaken in Denmark, Finland, Germany and Great Britain in Spring 2013. Within the international business model...... of format adaptation, the musical talent show genre has been particularly successful in crossing cultural borders. Formats such as Idols, X Factor and Voice have sold to a large variety of countries, covering all continents. Such global reach inevitably raises the question of the genre’s audience appeal...... that television formats to a considerable degree are adapted according to national audiences and, hence, national cultural tastes. And in our research approach, we do recognize that musical talent shows appear to contribute to ‘imagining the nation’ (Anderson 1983), often even mentioning the nation in the title...

  13. Reception of Talent Shows in Denmark: First Results from a Trans-National Audience Study of a Global Format Genre

    DEFF Research Database (Denmark)

    Jensen, Pia Majbritt

    of format adaptation, the musical talent show genre has been particularly successful in crossing cultural borders. Formats such as Idols, X Factor and Voice have sold to a large variety of countries, covering all continents. Such global reach inevitably raises the question of the genre’s audience appeal......This paper will discuss the methodology and present the preliminary findings of the Danish part of a trans-national, comparative audience study of the musical talent show genre undertaken in Denmark, Finland, Germany and Great Britain in Spring 2013. Within the international business model...... that television formats to a considerable degree are adapted according to national audiences and, hence, national cultural tastes. And in our research approach, we do recognize that musical talent shows appear to contribute to ‘imagining the nation’ (Anderson 1983), often even mentioning the nation in the title...

  14. Simulating cultural transmission: preliminary results and their implications for the study of formal variability of material culture in the Central Balkan Neolithic

    Directory of Open Access Journals (Sweden)

    Marko Porčić

    2014-12-01

    Full Text Available In this paper, we adopt the theoretical framework of evolutionary archaeology in order to model and simulate cultural transmission between hypothetical Neolithic sites in Balkans. We simulate neutral cultural transmission in order to compare the simulation results with empirically observed patterns of material culture variability such as traditional archaeological cultures. Our preliminary results show that a series of random local interactions can result in spatial groupings of typologically similar assemblages that correspond to the spatial distributions of traditional archaeological cultures, even in the absence of any other ‘external’ factor such as an overarching regional political structure or shared collective identity.

  15. Transcriptome profiling shows gene regulation patterns in a flavonoid pathway in response to exogenous phenylalanine in Boesenbergia rotunda cell culture.

    Science.gov (United States)

    Md-Mustafa, Noor Diyana; Khalid, Norzulaani; Gao, Huan; Peng, Zhiyu; Alimin, Mohd Firdaus; Bujang, Noraini; Ming, Wong Sher; Mohd-Yusuf, Yusmin; Harikrishna, Jennifer A; Othman, Rofina Yasmin

    2014-11-18

    Panduratin A extracted from Boesenbergia rotunda is a flavonoid reported to possess a range of medicinal indications which include anti-dengue, anti-HIV, anti-cancer, antioxidant and anti-inflammatory properties. Boesenbergia rotunda is a plant from the Zingiberaceae family commonly used as a food ingredient and traditional medicine in Southeast Asia and China. Reports on the health benefits of secondary metabolites extracted from Boesenbergia rotunda over the last few years has resulted in rising demands for panduratin A. However large scale extraction has been hindered by the naturally low abundance of the compound and limited knowledge of its biosynthetic pathway. Transcriptome sequencing and digital gene expression (DGE) analysis of native and phenylalanine treated Boesenbergia rotunda cell suspension cultures were carried out to elucidate the key genes differentially expressed in the panduratin A biosynthetic pathway. Based on experiments that show increase in panduratin A production after 14 days post treatment with exogenous phenylalanine, an aromatic amino acid derived from the shikimic acid pathway, total RNA of untreated and 14 days post-phenylalanine treated cell suspension cultures were extracted and sequenced using next generation sequencing technology employing an Illumina-Solexa platform. The transcriptome data generated 101, 043 unigenes with 50, 932 (50.41%) successfully annotated in the public protein databases; including 49.93% (50, 447) in the non-redundant (NR) database, 34.63% (34, 989) in Swiss-Prot, 24,07% (24, 316) in Kyoto Encyclopedia of Genes and Genomes (KEGG) and 16.26% (16, 426) in Clusters of Orthologous Groups (COG). Through DGE analysis, we found that 14, 644 unigenes were up-regulated and 14, 379 unigenes down-regulated in response to exogenous phenylalanine treatment. In the phenylpropanoid pathway leading to the proposed panduratin A production, 2 up-regulated phenylalanine ammonia-lyase (PAL), 3 up-regulated 4-coumaroyl

  16. Pharmacy Survey on Patient Safety Culture: Benchmarking Results.

    Science.gov (United States)

    Herner, Sheryl J; Rawlings, Julia E; Swartzendruber, Kelly; Delate, Thomas

    2017-03-01

    This study's objective was to assess the patient safety culture in a large, integrated health delivery system's pharmacy department to allow for benchmarking with other health systems. This was a cross-sectional survey conducted in a pharmacy department consisting of staff members who provide dispensing, clinical, and support services within an integrated health delivery system. The U.S. Agency for Healthcare Research and Quality's 11-composite, validated Pharmacy Survey on Patient Safety Culture questionnaire was transcribed into an online format. All departmental staff members were invited to participate in this anonymous survey. Cronbach α and overall results and contrasts between dispensing and clinical services staff and dispensing pharmacists and technicians/clerks as percentage positive scores (PPSs) are presented. Differences in contrasts were assessed with χ tests of association. Completed questionnaires were received from 598 (69.9%) of 855 employees. Cronbach α ranged from 0.55 to 0.90. Overall, the highest and lowest composite PPSs were for patient counseling (94.5%) and staffing and work pressure (44.7%), respectively. Compared with dispensing service, the clinical service participants had statistically higher PPSs for all composites except patient counseling, communication about mistakes, and staffing and work pressure (all P > 0.05). The technicians/clerks had a statistically higher PPS compared with the pharmacists for communication about mistakes (P = 0.007). All other composites were equivalent between groups. Patient counseling consistently had the highest PPS among composites measured, but opportunities existed for improvement in all aspects measured. Future research should identify and assess interventions targeted to improving the patient safety culture in pharmacy.

  17. THE COLORS OF THE OUTSKIRTS: THE AESTHETIC OF THE POPULAR CULTURE IN THE OPENING VIDEOCLIP OF THE "ESQUENTA!" TV SHOW

    Directory of Open Access Journals (Sweden)

    Regiane Regina Ribeiro

    2015-08-01

    Full Text Available Displayed by Globo TV since the year 2012, the tv show “Esquenta!” airs on Sundays as a popular attraction of drapery and dedicated to present on national television which is typical of the culture of Brazilian periphery. His introductory video clip, the object of analysis of this research, based on the use of some aesthetic elements to communicate to the Brazilian population that purpose to make the program into a showcase of the periphery on television. Based on image analysis, this article identifies framework elements, Figure and background, perspective and image composition that contribute to the identification of elements of popular within the program and the connection between this and your audience.

  18. Cells derived from porcine aorta tunica media show mesenchymal stromal-like cell properties in in vitro culture.

    Science.gov (United States)

    Zaniboni, Andrea; Bernardini, Chiara; Alessandri, Marco; Mangano, Chiara; Zannoni, Augusta; Bianchi, Francesca; Sarli, Giuseppe; Calzà, Laura; Bacci, Maria Laura; Forni, Monica

    2014-02-15

    Several studies have already described the presence of specialized niches of precursor cells in vasculature wall, and it has been shown that these populations share several features with mesenchymal stromal cells (MSCs). Considering the relevance of MSCs in the cardiovascular physiopathology and regenerative medicine, and the usefulness of the pig animal model in this field, we reported a new method for MSC-like cell isolation from pig aorta. Filling the vessel with a collagenase solution for 40 min, all endothelial cells were detached and discarded and then collagenase treatment was repeated for 4 h to digest approximately one-third of the tunica media. The ability of our method to select a population of MSC-like cells from tunica media could be ascribed in part to the elimination of contaminant cells from the intimal layer and in part to the overnight culture in the high antibiotic/antimycotic condition and to the starvation step. Aortic-derived cells show an elongated, spindle shape, fibroblast-like morphology, as reported for MSCs, stain positively for CD44, CD56, CD90, and CD105; stain negatively for CD34 and CD45; and express CD73 mRNA. Moreover, these cells show the classical mesenchymal trilineage differentiation potential. Under our in vitro culture conditions, aortic-derived cells share some phenotypical features with pericytes and are able to take part in the formation of network-like structures if cocultured with human umbilical vein endothelial cells. In conclusion, our work reports a simple and highly suitable method for obtaining large numbers of precursor MSC-like cells derived from the porcine aortic wall.

  19. [On the Way to Culture-Sensitive Patient Information Materials: Results of a Focus Group Study].

    Science.gov (United States)

    Ries, Zivile; Frank, Fabian; Bermejo, Isaac; Kalaitsidou, Chariklia; Zill, Jördis; Dirmaier, Jörg; Härter, Martin; Bengel, Jürgen; Hölzel, Lars

    2017-09-28

    Aim This study was part of a double-blind randomised controlled trial aimed to evaluate the effects of culture-sensitive patient information materials (PIM) compared with standard translated material. The study aimed to obtain the data for the development of culture sensitive PIM about unipolar depression for the 4 largest migrant groups in Germany (Turkish, Polish, Russian and Italian migration background). Method A qualitative study using 4 manual-based focus groups (FG), one for each migrant group, with 29 participants (9 with a Turkish (TüG), 8 with a Polish (PoG), 5 with a Russian (RuG) and 7 with an Italian (ItG) migration background) was conducted. The discussions were recorded, transcribed and analyzed using qualitative content analysis. Results 7 categories were identified. For the (1.) development of a good culture-sensitive PIM an easy language, a clear structure, an assessable extent of information and the avoidance of stereotypes were highlighted cross-culturally in all four FG. RuG and PoG had the largest (2.) lack of information about the German health care system. Concerning the (3.) illness perception RuG named problems with recognizing and understanding depression. PoG, RuG and TüG thematized (4.) feared consequences of the illness and of professional helpseeking. ItG, PoG, RuG had fears concerning (5.) psychotropic drugs as a result from insufficient knowledge about medication. For (6.) doctor-patient relationship cultural specifics were identified in RuG and TüG and for (7.) migration or culture specific reasons for depression in RuG, ItG and TüG. Conclusion Although the identified categories were relevant for all or for the majority of migrant groups, for most categories specific cultural aspects were discovered. These findings show the importance of a culture sensitive adaptation of PIM. © Georg Thieme Verlag KG Stuttgart · New York.

  20. Allotransplantation of cultured parathyroid progenitor cells without immunosuppression: clinical results.

    Science.gov (United States)

    Nawrot, Ireneusz; Woźniewicz, Bogdan; Tołłoczko, Tadeusz; Sawicki, Andrzej; Górski, Andrzej; Chudziński, Witold; Wojtaszek, Mikołaj; Grzesiuk, Wiesław; Sladowski, Dariusz; Karwacki, Jerzy; Zawitkowska, Teresa; Szmidt, Jacek

    2007-03-27

    Hypoparathyroidism is a well-known consequence of extensive thyroid and parathyroid surgery. Allotransplantation of cultured parathyroid cells can be considered as an alternative to vitamin D3 and calcium supplementation in treatment of hypoparathyroidism. We present the long-term allotransplant activity in 85 patients who had undergone cellular allotransplantation for surgical hypoparathyroidism. Also, a modified technique to prepare parathyroid explants is described for obtaining a new nonimmunogenic cell population. From March 1990 to December 2004, 85 patients underwent 116 allotransplantations of cultured parathyroid cells. Mean recipient age was 46.2+/-11.1 years. Donors were selected from patients undergoing parathyroidectomy for secondary and tertiary hyperparathyroidism. After 6 weeks of cultivation and freezing, the parathyroid cells decreased their normal human leukocyte antigen (HLA) class I ABC expression and were free of HLA class II positive cells. The viability of cultured cells was 95.15+/-2.94%. Eighty-five patients underwent primary allotransplantation. Of these, 25 patients subsequently underwent a repeat procedure. In six cases, the parathyroid cells were obtained from the same donor and in 19 cases from a different donor. For all patients, the mean cellular allograft survival was 6.35+/-13.08 months. In 64 patients (55.1%), the allografts retained their endocrine function for more than 2 months. The present study has shown that in some patients parathyroid cell allotransplantation may be considered a method of treatment for permanent hypoparathyroidism after thyroid surgery. Graft function and/or survival did not depend on the baseline viability or secretory activity of cultured cells used for transplantation.

  1. Effect of Semi-quantitative Culture Results from Complex Host Surgical Wounds on Dehiscence Rates.

    Science.gov (United States)

    Elmarsafi, Tammer; Garwood, Caitlin S; Steinberg, John S; Evans, Karen K; Attinger, Christopher E; Kim, Paul J

    2017-01-16

    The primary aim of this study was to determine the effect of positive bacterial cultures at the time of closure on dehiscence rates. Pre and post-débridement wound cultures from patients undergoing serial surgical débridement of infected wounds were compared with outcomes 30 days postoperatively. One-hundred patients were enrolled; 35 were excluded for incomplete culture data. Sixty-five patients were evaluated for species counts, including Coagulase negative Staphylococcus, and semi-quantitative culture data for each débridement. The post-débridement cultures on the date of closure had no growth in 42 patients (64.6%) of which 6 dehisced (14.3%), and 36 remained closed; with no statistically significant difference in dehiscence rates (p=0.0664). Pre-débridement cultures from the 1(st) débridement of the 65 patients showed 8 patients had no growth, 29 grew 1 species, 19 grew 2 species, and 9 had 3-5 species. There was a reduction in the number of species and improvement of semi-quantitative cultures with each subsequent débridement. The dehiscence rate for those who had 2 débridements (n=42) was 21.4% at 30 day follow-up and 21.7% in those who had 3 débridements (n=23). The number of débridements had no statistical significance on dehiscence rates. The presence of Coagulase negative Staphylococcus (CoNS) on the day of closure was a statistically significant risk for dehiscence within 30 days (p=0.0091) postoperatively. This data demonstrates: (1) positive post-débridement cultures (scant/rare, growth in enrichment broth) at the time of closure did not affect overall dehiscence rates (p=0.0664), (2) the number of species and semi-quantitiative culture results both improved with each subsequent débridement, (3) the number of surgical débridement did not influence post-closure dehiscence rates. (4) Positive cultures containing Coagulase negative Staphylococcus at the time of closure is a risk factor for dehiscence (p=0.0091). This article is protected by

  2. Copper incorporation in foraminiferal calcite: results from culturing experiments

    Directory of Open Access Journals (Sweden)

    G. J. van der Zwaan

    2007-07-01

    Full Text Available A partition coefficient for copper (DCu in foraminiferal calcite has been determined by culturing individuals of two benthic species under controlled laboratory conditions. The partition coefficient of a trace element (TE is an emperically determined relation between the TE/Ca ratio in seawater and the TE/Ca ratio in foraminiferal calcite and has been established for many divalent cations. Despite its potential to act as a tracer of human-induced, heavy metal pollution, data is not yet available for copper. Since partition coefficients are usually a function of multiple factors (seawater temperature, pH, salinity, metabolic activity of the organism, etc., we chose to analyze calcite from specimens cultured under controlled laboratory conditions. They were subjected to different concentrations of Cu2+ (0.1–20 µmol/l and constant temperature (10 and 20°C, seawater salinity and pH. We monitored the growth of new calcite in specimens of the temperate, shallow-water foraminifer Ammonia tepida and in the tropical, symbiont-bearing Heterostegina depressa. Newly formed chambers were analyzed for Cu/Ca ratios by laser ablation-ICP-MS. The estimated partition coefficient (0.1–0.4 was constant to within experimental error over a large range of (Cu/Caseawater ratios and was remarkably similar for both species. Neither did the presence or absence of symbionts affect the DCu, nor did we find a significant effect of temperature or salinity on Cu-uptake.

  3. Gene expression profiles of mouse aorta and cultured vascular smooth muscle cells differ widely, yet show common responses to dioxin exposure.

    Science.gov (United States)

    Puga, Alvaro; Sartor, Maureen A; Huang, Ming-Ya; Kerzee, J Kevin; Wei, Yu-Dan; Tomlinson, Craig R; Baxter, C Stuart; Medvedovic, Mario

    2004-01-01

    Exposure to environmental toxicants may play a role in the onset and progression of cardiovascular disease. Many environmental agents, such as dioxin, are risk factors for atherosclerosis because they may exacerbate an underlying disease by altering gene expression patterns. Expression profiling of vascular tissues allows the simultaneous analysis of thousands of genes and may provide predictive information particularly useful in early disease stages. Often, however, in vivo experiments are unfeasible for material or ethical reasons, and data from cultured cells must be used instead, even though it may not be known whether cultured cells and live tissues share common global responses to the same toxicant. In a search for genes responsive to dioxin exposure, we used oligonucleotide microarrays with DNA sequences from 13,433 genes to compare global gene expression profiles of C57BL/6 mice aortas with cultured vascular smooth muscle cells (vSMCs) of the same mice. Aorta segments and vSMCs differed in the expression of more than 4500 genes, many showing expression differences greater than 1000-fold. Integration of microarray data into Gene Ontology Project annotations showed that many of the genes differentially expressed belonged to the same biological process or metabolic pathway. Notwithstanding these results, a subset of 35 genes responded in the same fashion to dioxin exposure in both systems. Genes in this subset encoded phase I and phase II detoxification enzymes, signal transduction kinases and phosphatases, and proteins involved in DNA repair and the cell cycle. We conclude that vSMCS may be useful aorta surrogates to study early gene expression responses to dioxin exposure, provided that analyses focus on this subset of genes.

  4. Rapid reporting of urine culture results: impact of the uro-quick screening system.

    Science.gov (United States)

    Ilki, Arzu; Bekdemir, Pinar; Ulger, Nurver; Soyletir, Guner

    2010-04-01

    This study evaluated the impact of the Uro-Quick (UQ) screening system (Alifax, Italy) for a rapid and accurate reporting of urine cultures, and whether it can provide bacterial yield to be used in identification and susceptibility testing. A total of 1480 urine samples collected between October 2006 and July 2008 were tested by conventional culture (CC) methods and UQ simultaneously. Sediments of positive UQ vials were used as bacterial yields for identification and susceptibility testing procedures. Of the 1480 samples, 999 revealed bacteria and/or leukocytes in direct microscopy. Among these 999 samples, positive growth was detected in 420 (42%) and 433 (43.3%) by UQ and CC, respectively. However, only 0.6% of samples without bacteria and leukocytes exhibited positive growth. When compared to CC, UQ demonstrated high levels of positive predictive value (95.9%), negative predictive value (94.8%), sensitivity (93%) and specificity (96.9%). Both CC isolates and UQ bacteria showed 81.3% concordance in identification results. Susceptibility testing of UQ bacteria displayed >90% agreement, when compared with standardized disk diffusion test. Our results indicate that UQ can reliably be used in routine laboratories giving microbial growth results in 3 hours. The most significant part of the study is that bacterial yields of UQ positive samples can be used in identification and susceptibility testing, allowing a rapid, same-day reporting of urine cultures.

  5. STARtorialist: Astronomy Fashion & Culture Blog and Reader Survey Results

    Science.gov (United States)

    Ash, Summer; Rice, Emily L.; Jarreau, Paige B.

    2016-01-01

    STARtorialist (startorialist.com) is a Tumblr-based blog that curates the proliferation of "Astro Fashion" - clothing accessories, decor, and more - with the goal of celebrating the beauty of the universe and highlighting the science behind the astronomical imagery. Since launching in January 2013, we have written over 1000 unique posts about everything from handmade and boutique products to mass-produced commercial items to haute couture seen on fashion runways. Each blog post features images and descriptions of the products with links to the original astronomical images or other relevant science content. We also feature profiles of astronomers, scientists, students, and communicators wearing, making, or decorating with "startorial" items. Our most popular posts accumulate hundreds or thousands of notes (faves or reblogs, in Tumblr parlance), and one post has nearly 150,000 notes. In our second year, we have grown from 1,000 to just shy of 20,000 followers on Tumblr, with an increased audience on Twitter, Instagram, and Facebook as well. We present preliminary results from a reader survey conducted September-October 2015 in collaboration with science communication researcher Dr. Paige Jarreau. The survey provides data on reader habits, motivations, attitudes, and demographics in order to assess how STARtorialist has influenced our readers' views on science, scientists, and the scientific community as a whole.

  6. Building an Evaluative Culture: The Key to Effective Evaluation and Results Management

    Science.gov (United States)

    Mayne, John

    2009-01-01

    As many reviews of results-based performance systems have noted, a weak evaluative culture in an organization undermines attempts at building an effective evaluation and/or results management regime. This article sets out what constitutes a strong evaluative culture where information on performance results is deliberately sought in order to learn…

  7. Astrocyte Cultures Mimicking Brain Astrocytes in Gene Expression, Signaling, Metabolism and K(+) Uptake and Showing Astrocytic Gene Expression Overlooked by Immunohistochemistry and In Situ Hybridization.

    Science.gov (United States)

    Hertz, Leif; Chen, Ye; Song, Dan

    2017-01-01

    Based on differences in gene expression between cultured astrocytes and freshly isolated brain astrocytes it has been claimed that cultured astrocytes poorly reflect the characteristics of their in vivo counterparts. This paper shows that this is not the case with the cultures of mouse astrocytes we have used since 1978. The culture is prepared following guidelines provided by Drs. Monique Sensenbrenner and John Booher, with the difference that dibutyryl cyclic AMP is added to the culture medium from the beginning of the third week. This addition has only minor effects on glucose and glutamate metabolism, but it is crucial for effects by elevated K(+) concentrations and for Ca(2+) homeostasis, important aspects of astrocyte function. Work by Liang Peng and her colleagues has shown identity between not only gene expression but also drug-induced gene upregulations and editings in astrocytes cultured by this method and astrocytes freshly isolated from brains of drug-treated animals. Dr. Norenberg's laboratory has demonstrated identical upregulation of the cotransporter NKCC1 in ammonia-exposed astrocytes and rats with liver failure. Similarity between cultured and freshly isolated astrocytes has also been shown in metabolism, K(+) uptake and several aspects of signaling. However, others have shown that the gene for the glutamate transporter GLT1 is not expressed, and rat cultures show some abnormalities in K(+) effects. Nevertheless, the overall reliability of the cultured cells is important because immunohistochemistry and in situ hybridization poorly demonstrate many astrocytic genes, e.g., those of nucleoside transporters, and even microarray analysis of isolated cells can be misleading.

  8. A weight-loss intervention program designed for Mexican-American women: Cultural adaptations and results

    Science.gov (United States)

    Lindberg, Nangel M.; Stevens, Victor J.; Vega-López, Sonia; Kauffman, Tia; Calderón, Mariana Rosales; Cervantes, María Antonieta

    2013-01-01

    Background This study assessed the feasibility of a culturally-appropriate weight-loss intervention targeting obese Spanish-speaking Mexican women. Methods This 12-month weight-loss program was based on behavioral interventions previously used successfully with English-speaking participants. Cultural adaptations included: female interventionists, minimal written materials, emphasis on group activities, focus on Mexican traditions and beliefs, and skill-building approach to food measurement. All sessions were conducted in Spanish. The study had few exclusionary criteria, which allowed participation of women with a wide range of literacy levels. Results Recruitment exceeded expectations, with 47 participants enrolling in the program. Not counting participants who became pregnant during the study, attendance at 6 and 12 months was 62% and 50% respectively. Mean weight loss at 6 and 12 months was 5.3 kg and 7.2 kg, respectively, with a mean reduction in BMI of 4.0 kg/m2 and 5.5 kg/m2 from baseline to 6 and 12 months, respectively. Discussion This pilot study shows that it is feasible to develop and implement culturally-appropriate behavioral lifestyle interventions for obesity treatment in Mexican-American women. PMID:22460538

  9. "Showing roughness in a beautiful way": talk about love, coercion, and rape in South African youth sexual culture.

    Science.gov (United States)

    Wood, Kate; Lambert, Helen; Jewkes, Rachel

    2007-09-01

    Sexual violence within as well as outside sexual relationships has far-reaching public health and human rights implications and is a continuing focus of popular debate, media coverage, and research in postapartheid South Africa. Partly because it has been shown to affect individual vulnerability to HIV/AIDS, sexual violence has in recent years become framed as a global public health issue. International research efforts to document the scale of this personally and politically sensitive problem can encounter conceptual, definitional, and methodological difficulties that anthropology is well placed to assist in alleviating. This article offers an ethnographic exploration of the spectrum of practices relating to sexual coercion and rape among young people in a township in the former Transkei region of South Africa. Contextualizing meanings of sexual coercion within local youth sexual culture, the article considers two emic categories associated with sex that is "forced": ukulala ngekani: "to sleep with by force" or ukunyanzela: "to force," both usually used to describe episodes occurring within sexual partnerships; and ukudlwengula, used to describe rape by a nonpartner or stranger. The article discusses the semantic content of and differences between these two key categories, demonstrating that encounters described as "forced sex" encompass not only various forms of sexual coercion but also, particularly in the narratives of young men, instances of more consensual sex. Of importance, in turn, in defining an act as "rape" rather than as "forced sex" are the character of the relationship between the two parties and interlinked ideas relating to exchange and sexual entitlement, love, and the importance of "intention," violation, and "deserving" victimhood.

  10. Reliability of environmental sampling culture results using the negative binomial intraclass correlation coefficient.

    Science.gov (United States)

    Aly, Sharif S; Zhao, Jianyang; Li, Ben; Jiang, Jiming

    2014-01-01

    The Intraclass Correlation Coefficient (ICC) is commonly used to estimate the similarity between quantitative measures obtained from different sources. Overdispersed data is traditionally transformed so that linear mixed model (LMM) based ICC can be estimated. A common transformation used is the natural logarithm. The reliability of environmental sampling of fecal slurry on freestall pens has been estimated for Mycobacterium avium subsp. paratuberculosis using the natural logarithm transformed culture results. Recently, the negative binomial ICC was defined based on a generalized linear mixed model for negative binomial distributed data. The current study reports on the negative binomial ICC estimate which includes fixed effects using culture results of environmental samples. Simulations using a wide variety of inputs and negative binomial distribution parameters (r; p) showed better performance of the new negative binomial ICC compared to the ICC based on LMM even when negative binomial data was logarithm, and square root transformed. A second comparison that targeted a wider range of ICC values showed that the mean of estimated ICC closely approximated the true ICC.

  11. The performance of fully automated urine analysis results for predicting the need of urine culture test

    Directory of Open Access Journals (Sweden)

    Hatice Yüksel

    2014-06-01

    Full Text Available Objectives: Urinalysis and urine culture are most common tests for diagnosis of urinary tract infections. The aim of our study is to examine the diagnostic performance of urine analysis and the role of urine analysis to determine the requirements for urine culture. Methods: Urine culture and urine analysis results of 362 patients were retrospectively analyzed. Culture results were taken as a reference for chemical and microscopic examination of urine and diagnostic accuracy of the test parameters, that may be a marker for urinary tract infection, and the performance of urine analysis were calculated for predicting the urine culture requirements. Results: A total of 362 urine culture results of patients were evaluated and 67% of them were negative. The results of leukocyte esterase and nitrite in chemical analysis and leukocytes and bacteria in microscopic analysis were normal in 50.4% of culture negative urines. In diagnostic accuracy calculations, leukocyte esterase (86.1% and microscopy leukocytes (88.0% were found with high sensitivity, nitrite (95.4% and bacteria (86.6% were found with high specificity. The area under the curve was calculated as 0.852 in ROC analysis for microscopic examination for leukocytes. Conclusion: Full-automatic urine devices can provide sufficient diagnostic accuracy for urine analysis. The evaluation of urine analysis results in an effective way can predict the necessity for urine culture requests and especially may contribute to a reduction in the work load and cost. J Clin Exp Invest 2014; 5 (2: 286-289

  12. THE COLOMBIAN REALITY FROM THE PERSPECTIVE OF BEHAIOVR ANALYSIS: PEACE AS A RESULT OF CULTURAL PRACTICES

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    BLANCA PATRICIA BALLESTEROS DE VALDERRAMA

    2002-01-01

    Full Text Available This article presents the advantage of applying behavior analysis to cultural practices comprehension, considering cultural practices as group behaviors, selected and maintained by social reinforcement contingencies and metacontingencies. The three types of contingencies formulated by Skinner are described, and the principal existing data related to aggressive behavior. A proposal to analyze some relevant aspects to Colombian reality is formulated, and finally, an intent to analyze peace as a result of cultural practices is presented, emphasizing positive reciprocal social processes. Mattaini's (1996 suggestion to graphical representation of cultural practices analysis is applied.

  13. Transgenic plants expressing HC-Pro show enhanced virus sensitivity while silencing of the transgene results in resistance

    NARCIS (Netherlands)

    Mlotshwa, S.; Verver, J.; Sithole-Niang, I.; Prins, M.; Kammen, van A.; Wellink, J.

    2002-01-01

    Nicotiana benthamiana plants were engineered to express sequences of the helper component-proteinase (HC-Pro) of Cowpea aphid-borne mosaic potyvirus (CABMV). The sensitivity of the transgenic plants to infection with parental and heterologous viruses was studied. The lines expressing HC-Pro showed

  14. High Content Analysis of Hippocampal Neuron-Astrocyte Co-cultures Shows a Positive Effect of Fortasyn Connect on Neuronal Survival and Postsynaptic Maturation

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    Anne-Lieke F. van Deijk

    2017-08-01

    Full Text Available Neuronal and synaptic membranes are composed of a phospholipid bilayer. Supplementation with dietary precursors for phospholipid synthesis –docosahexaenoic acid (DHA, uridine and choline– has been shown to increase neurite outgrowth and synaptogenesis both in vivo and in vitro. A role for multi-nutrient intervention with specific precursors and cofactors has recently emerged in early Alzheimer's disease, which is characterized by decreased synapse numbers in the hippocampus. Moreover, the medical food Souvenaid, containing the specific nutrient combination Fortasyn Connect (FC, improves memory performance in early Alzheimer's disease patients, possibly via maintaining brain connectivity. This suggests an effect of FC on synapses, but the underlying cellular mechanism is not fully understood. Therefore, we investigated the effect of FC (consisting of DHA, eicosapentaenoic acid (EPA, uridine, choline, phospholipids, folic acid, vitamins B12, B6, C and E, and selenium, on synaptogenesis by supplementing it to primary neuron-astrocyte co-cultures, a cellular model that mimics metabolic dependencies in the brain. We measured neuronal developmental processes using high content screening in an automated manner, including neuronal survival, neurite morphology, as well as the formation and maturation of synapses. Here, we show that FC supplementation resulted in increased numbers of neurons without affecting astrocyte number. Furthermore, FC increased postsynaptic PSD95 levels in both immature and mature synapses. These findings suggest that supplementation with FC to neuron-astrocyte co-cultures increased both neuronal survival and the maturation of postsynaptic terminals, which might aid the functional interpretation of FC-based intervention strategies in neurological diseases characterized by neuronal loss and impaired synaptic functioning.

  15. Maraviroc shows differential effects on glucose uptake and lipolysis in human subcutaneous cultured adipocytes in comparison with omental adipocytes

    Directory of Open Access Journals (Sweden)

    P Perez-Matute

    2012-11-01

    Full Text Available Maraviroc (MVC, the first approved CC-chemokine receptor 5 (CCR5 antagonist, is used for treating HIV-1-infected patients with CCR5 tropism. MVC has been proved safe in all respects and showed beneficial effects on lipid profile of HIV patients with dislipidemia. Adipocyte dysfunction seems to be responsible for many metabolic alterations such as insulin resistance and dislipidemia. Subcutaneous and visceral fat depots are not only physiologically but also metabolically different and metabolic disturbances are more closely associated with visceral than subcutaneous fat accumulation. It has been suggested that antiretrovirals affect both fat depots in a different extent. Thus, whether isolated human adipocytes display regio-specific sensitivity to the metabolic effects of MVC have been tested in this study. Human subcutaneous and omental preadipocytes were used as the source of human adipocytes. These cells were treated with therapeutic concentrations of MVC (0.5–25 µM at day 14 post-differentiation (4 and 24 hours of treatment. Glucose utilization, lactate production and glycerol released into the media were measured using an autoanalyzer. Adiponectin secretion was determined by an ELISA array. A dose-dependent increase in glucose uptake was observed in subcutaneous adipocytes treated with MVC (+72% of stimulation for MVC 25 µM, p < 0.01. This stimulatory effect was tissue specific, as no effects were observed in omental adipocytes. MVC did not exert any significant effect on adiponectin secretion. No significant effects were observed on lactate production neither in subcutaneous nor omental adipocytes. Interestingly, 4 hours of treatment with MVC induced a significant increase in the amount of glycerol released into the media by subcutaneous adipocytes (p < 0.001, but this effect disappeared with longer exposure of adipocytes to MVC (24 h. No effects were observed on lipolysis in omental adipocytes although a slight tendency to

  16. Early Results Show Reduced Infection Rate Using No-touch Technique for Expander/ADM Breast Reconstruction

    OpenAIRE

    Henry B. Wilson, MD, FACS

    2015-01-01

    Summary: Infection is a common complication of immediate breast reconstruction that often leads to device removal, a result emotionally devastating to the patient and frustrating for her surgeon. “No-touch” techniques have been used in other surgical disciplines and plastic surgery, but they have not been reported for breast reconstruction with tissue expanders or implants and acellular dermis. We report a novel technique of tissue expander and acellular dermis placement using no-touch princi...

  17. Early Results Show Reduced Infection Rate Using No-touch Technique for Expander/ADM Breast Reconstruction

    Directory of Open Access Journals (Sweden)

    Henry B. Wilson, MD, FACS

    2015-03-01

    Full Text Available Summary: Infection is a common complication of immediate breast reconstruction that often leads to device removal, a result emotionally devastating to the patient and frustrating for her surgeon. “No-touch” techniques have been used in other surgical disciplines and plastic surgery, but they have not been reported for breast reconstruction with tissue expanders or implants and acellular dermis. We report a novel technique of tissue expander and acellular dermis placement using no-touch principles with a self-retaining retractor system that holds promise to decrease infectious complications of breast reconstruction.

  18. Mixed culture biofilms of Salmonella Typhimurium and cultivable indigenous microorganisms on lettuce show enhanced resistance of their sessile cells to cold oxygen plasma.

    Science.gov (United States)

    Jahid, Iqbal Kabir; Han, Noori; Zhang, Cheng-Yi; Ha, Sang-Do

    2015-04-01

    Control of foodborne pathogens in fresh produce is crucial for food safety, and numerous Salmonella Typhimurium (ST) outbreaks have been reported already. The present study was done to assess effectiveness of cold oxygen plasma (COP) against biofilms of ST mixed with cultivable indigenous microorganisms (CIM). ST and CIM were grown at 15 °C as monocultures and mixed cultures for planktonic state, biofilm on stainless steel, and lettuce leaves. Thereafter, the samples were treated with COP and surviving populations were counted using plate counting methods. Biofilms and stomatal colonization were examined using field emission scanning electron microscopy (FESEM) and food quality was assessed after treatment. Mixed cultures of ST and CIM showed an antagonistic interaction on lettuce but not on SS or in planktonic state. Mixed cultures showed significantly (p biofilms on lettuce but not on SS or planktonic state. Shift from smooth to rugose colony type was found for planktonic and for biofilms on SS but not on lettuce for ST. Mixed culture biofilms colonized stomata on the inside as demonstrated by FESEM. Although, lettuce quality was not affected by COP, this technology has to be optimized for further development of the successful inactivation of complex multispecies biofilm structures presented by real food environment.

  19. Cultural adaptation and validation of an instrument on barriers for the use of research results.

    Science.gov (United States)

    Ferreira, Maria Beatriz Guimarães; Haas, Vanderlei José; Dantas, Rosana Aparecida Spadoti; Felix, Márcia Marques Dos Santos; Galvão, Cristina Maria

    2017-03-02

    to culturally adapt The Barriers to Research Utilization Scale and to analyze the metric validity and reliability properties of its Brazilian Portuguese version. methodological research conducted by means of the cultural adaptation process (translation and back-translation), face and content validity, construct validity (dimensionality and known groups) and reliability analysis (internal consistency and test-retest). The sample consisted of 335 nurses, of whom 43 participated in the retest phase. the validity of the adapted version of the instrument was confirmed. The scale investigates the barriers for the use of the research results in clinical practice. Confirmatory factorial analysis demonstrated that the Brazilian Portuguese version of the instrument is adequately adjusted to the dimensional structure the scale authors originally proposed. Statistically significant differences were observed among the nurses holding a Master's or Doctoral degree, with characteristics favorable to Evidence-Based Practice, and working at an institution with an organizational cultural that targets this approach. The reliability showed a strong correlation (r ranging between 0.77 and 0.84, pcultura organizacional dirigida hacia tal aproximación. La fiabilidad presentó correlación fuerte (r variando entre 0,77 y 0,84, pcultura organizacional direcionada para tal abordagem. A confiabilidade apresentou correlação forte (r variando entre 0,77e 0,84, p<0,001) e a consistência interna foi adequada (alfa de Cronbach variando entre 0,77 e 0,82) . a versão para o português brasileiro do instrumento The Barriers Scale demonstrou-se válida e confiável no grupo estudado.

  20. Selection Indices and Multivariate Analysis Show Similar Results in the Evaluation of Growth and Carcass Traits in Beef Cattle.

    Science.gov (United States)

    Brito Lopes, Fernando; da Silva, Marcelo Corrêa; Magnabosco, Cláudio Ulhôa; Goncalves Narciso, Marcelo; Sainz, Roberto Daniel

    2016-01-01

    This research evaluated a multivariate approach as an alternative tool for the purpose of selection regarding expected progeny differences (EPDs). Data were fitted using a multi-trait model and consisted of growth traits (birth weight and weights at 120, 210, 365 and 450 days of age) and carcass traits (longissimus muscle area (LMA), back-fat thickness (BF), and rump fat thickness (RF)), registered over 21 years in extensive breeding systems of Polled Nellore cattle in Brazil. Multivariate analyses were performed using standardized (zero mean and unit variance) EPDs. The k mean method revealed that the best fit of data occurred using three clusters (k = 3) (P multivariate index (LD1) were moderate to high, ranging from 0.48 to 0.97. This reveals that both types of indices give similar results and that the multivariate approach is reliable for the purpose of selection. The alternative tool seems very handy when economic weights are not available or in cases where more rapid identification of the best animals is desired. Interestingly, multivariate analysis allowed forecasting information based on the relationships among breeding values (EPDs). Also, it enabled fine discrimination, rapid data summarization after genetic evaluation, and permitted accounting for maternal ability and the genetic direct potential of the animals. In addition, we recommend the use of longissimus muscle area and subcutaneous fat thickness as selection criteria, to allow estimation of breeding values before the first mating season in order to accelerate the response to individual selection.

  1. Low-frequency ac electroporation shows strong frequency dependence and yields comparable transfection results to dc electroporation.

    Science.gov (United States)

    Zhan, Yihong; Cao, Zhenning; Bao, Ning; Li, Jianbo; Wang, Jun; Geng, Tao; Lin, Hao; Lu, Chang

    2012-06-28

    Conventional electroporation has been conducted by employing short direct current (dc) pulses for delivery of macromolecules such as DNA into cells. The use of alternating current (ac) field for electroporation has mostly been explored in the frequency range of 10kHz-1MHz. Based on Schwan equation, it was thought that with low ac frequencies (10Hz-10kHz), the transmembrane potential does not vary with the frequency. In this report, we utilized a flow-through electroporation technique that employed continuous 10Hz-10kHz ac field (based on either sine waves or square waves) for electroporation of cells with defined duration and intensity. Our results reveal that electropermeabilization becomes weaker with increased frequency in this range. In contrast, transfection efficiency with DNA reaches its maximum at medium frequencies (100-1000Hz) in the range. We postulate that the relationship between the transfection efficiency and the ac frequency is determined by combined effects from electrophoretic movement of DNA in the ac field, dependence of the DNA/membrane interaction on the ac frequency, and variation of transfection under different electropermeabilization intensities. The fact that ac electroporation in this frequency range yields high efficiency for transfection (up to ~71% for Chinese hamster ovary cells) and permeabilization suggests its potential for gene delivery.

  2. Selection Indices and Multivariate Analysis Show Similar Results in the Evaluation of Growth and Carcass Traits in Beef Cattle.

    Directory of Open Access Journals (Sweden)

    Fernando Brito Lopes

    Full Text Available This research evaluated a multivariate approach as an alternative tool for the purpose of selection regarding expected progeny differences (EPDs. Data were fitted using a multi-trait model and consisted of growth traits (birth weight and weights at 120, 210, 365 and 450 days of age and carcass traits (longissimus muscle area (LMA, back-fat thickness (BF, and rump fat thickness (RF, registered over 21 years in extensive breeding systems of Polled Nellore cattle in Brazil. Multivariate analyses were performed using standardized (zero mean and unit variance EPDs. The k mean method revealed that the best fit of data occurred using three clusters (k = 3 (P < 0.001. Estimates of genetic correlation among growth and carcass traits and the estimates of heritability were moderate to high, suggesting that a correlated response approach is suitable for practical decision making. Estimates of correlation between selection indices and the multivariate index (LD1 were moderate to high, ranging from 0.48 to 0.97. This reveals that both types of indices give similar results and that the multivariate approach is reliable for the purpose of selection. The alternative tool seems very handy when economic weights are not available or in cases where more rapid identification of the best animals is desired. Interestingly, multivariate analysis allowed forecasting information based on the relationships among breeding values (EPDs. Also, it enabled fine discrimination, rapid data summarization after genetic evaluation, and permitted accounting for maternal ability and the genetic direct potential of the animals. In addition, we recommend the use of longissimus muscle area and subcutaneous fat thickness as selection criteria, to allow estimation of breeding values before the first mating season in order to accelerate the response to individual selection.

  3. Results of Performing Blood Cultures at Hospital Admission in Patients with Community-acquired Pneumonia

    Directory of Open Access Journals (Sweden)

    Iris González Morales

    2014-02-01

    Full Text Available Background: community-acquired pneumonia is a major health problem worldwide, in Cuba and in the province of Cienfuegos. Objective: to report the results of blood cultures performed at hospital admission in patients with community-acquired pneumonia. Methods: a prospective descriptive study was conducted in the Gustavo Aldereguía Lima Hospital in Cienfuegos, during the second half of 2012. It included 52 patients with community-acquired pneumonia who underwent blood culture at their admission to the hospital. Results: only five patients (9.6% of the cases had a positive test result; Streptococcus pneumoniae was isolated from only one positive culture; Staphylococcus aureus and Klebsiella pneumoniae were isolated from the rest. Conclusions: the percentage obtained in this study confirms the low diagnostic yield of blood cultures performed at admission in patients with community-acquired pneumonia; the low isolation rate of S. pneumoniae in our study was also significant.

  4. PERCEPTION OF FOREIGN DIRECT INVESTMENT AS A RESULT OF CULTURAL DIFFERENTIATION

    Directory of Open Access Journals (Sweden)

    Sebastian D. TOCAR

    2017-06-01

    Full Text Available The impact of foreign direct investment (FDI on the economy is difficult to overestimate. The influence of cultural factors on FDI is recognized in the scientific community. There are attempts to approach the subject, although they are usually superficial and focused on a certain side of the relation, qualitative analysis is rarely used. The paper presents a qualitative approach to the link between the country's culture and FDI. We propose the results of content analysis of the articles published in three countries: Romania, Ukraine and Moldova, which aim to highlight differences in the vision of FDI as a result of cultural differentiation and cohere with the amount of FDI. The research results aim to underline the role of cultural differentiation between the countries.

  5. Determining the Clinical Utility of an Absolute Procalcitonin Value for Predicting a Positive Culture Result.

    Science.gov (United States)

    Caffarini, Erica M; DeMott, Joshua; Patel, Gourang; Lat, Ishaq

    2017-05-01

    Various procalcitonin ranges have been established to guide antimicrobial therapy; however, there are no data that establish whether the initial procalcitonin value can determine the likelihood of a positive culture result. This study aimed to establish if the initial procalcitonin value, on clinical presentation, has a positive predictive value for any positive culture result. This was a retrospective study of 813 medical intensive care unit patients. Data collected included patient demographics, procalcitonin assay results, sources of infection, culture results, and lengths of stay. Patients were excluded if they were immunocompromised. The primary outcome of this study was to determine a procalcitonin value that would predict any positive culture. Secondary outcomes included the sensitivity, specificity, positive predictive value, and negative predictive value for procalcitonin. After exclusions, a total of 519 patient charts were reviewed to determine the impact of the initial procalcitonin value on culture positivity. In our analyses, the receiver operating characteristic values were 0.62 for all cultures, 0.49 for pulmonary infections, 0.43 for urinary tract infections, and 0.78 for bacteremia. A procalcitonin value of 3.61 ng/ml was determined to be the threshold value for a positive blood culture result (prevalence, 4%). For bacteremia, the sensitivity of procalcitonin was 75%, the specificity was 72%, the positive predictive value was 20%, and the negative predictive value was 97%. Procalcitonin was a poor predictor of culture positivity. An initial procalcitonin value of less than 3.61 ng/ml may be useful in predicting whether bacteremia is absent. Procalcitonin should not be used as the only predictor for determining initiation of antibiotic therapy. Copyright © 2017 American Society for Microbiology.

  6. Getting carried away: a note showing baseline observation carried forward (BOCF) results can be calculated from published complete-cases results.

    Science.gov (United States)

    Kaiser, K A; Affuso, O; Beasley, T M; Allison, D B

    2012-06-01

    Randomized controlled trials (RCTs) in obesity are plagued by missing data due to participant dropouts. Most methodologists and regulatory bodies agree that the primary analysis of such RCTs should be based on the intent-to-treat (ITT) principle, such that all randomized subjects are included in the analysis, even those who dropped out. Unfortunately, some authors do not include an ITT analysis in their published reports. Here we show that one form of ITT analysis, baseline observation carried forward (BOCF), can be performed utilizing only information available in a published complete-case (CC) analysis, permitting readers, editors, meta-analysts and regulators to easily conduct their own ITT analyses when the original authors do not report one. We mathematically derive a simple method for estimating and testing treatment effects using the BOCF to allow a more conservative comparison of treatment effects when there are dropouts in a clinical trial. We provide two examples of this method using available CC analysis data from reported obesity trials to illustrate the application for readers who wish to determine a range of treatment effects based on published summary statistics. Commonly used CC analyses may lead to inflated type I error rates and/or treatment effect estimates. The method described herein can be useful for researchers who wish to estimate a conservative range of plausible treatment effects based on limited reported data. Limitations of this method are discussed.

  7. Renegotiating cultural practices as a result of HIV in the eastern region of Malawi.

    Science.gov (United States)

    Banda, Felix; Kunkeyani, Thokozani E

    2015-01-01

    A number of studies have shown that HIV awareness is very high among Malawians and yet infection rates are rising. Local cultural practices have been identified as contributing to this contradictory situation. Using data from 12 focus-group discussions collected in Balaka, Zomba, Machinga and Mangochi, the paper explores the reformulation of nine cultural practices as a preventive measure against HIV. The study reveals that cultural practices that involve sexual acts for completion are mediated through condoms and HIV tests. The study also shows that traditional herbs known for healing ailments are repurposed to symbolise sexual acts. We conclude that the idea of repurposing offers an avenue in which initiation and cleansing rites that involve sexual acts are replaced by other semiotics such as a traditional medicine called mtela. We also conclude that the modifications to cultural practices do not indicate complete abandonment of associated traditions, rather, they constitute the renegotiation of cultural practices and meanings associated with particular rites of passage. Lastly, we propose that a comprehensive prevention programme needs to be part of a wider national HIV-prevention effort combining a women and child rights and empowerment agenda and, critically, lifestyle lessons in a process of cultural renegotiation.

  8. Illegal and legal parrot trade shows a long-term, cross-cultural preference for the most attractive species increasing their risk of extinction.

    Directory of Open Access Journals (Sweden)

    José L Tella

    Full Text Available Illegal trade constitutes a major threat for a variety of wildlife. A criminology framework has been recently applied to parrot poaching in Mexico, suggesting an opportunistic crime in which the most abundant and accessible species, and not the rare or highly priced species, were poached more often. We analyzed this information, together with additional long-term data (1981-2005 on both the legal and illegal trade of the 22 Mexican parrot species (n = 31,019 individuals, using multivariate statistics and hypothesis-testing approaches. Our results showed a selective capture of parrot species attending to their attractiveness. Parrot species widely differed in attractiveness to people (as reflected by their combined measures of body size, coloration, and ability to imitate human speech, and their attractiveness strongly correlated with their prices both in the Mexican and US markets. The most attractive and valuable species (amazons and macaws were disproportionally caught attending to the number of years they were legally trapped. Similar patterns were found for parrots poached for the domestic Mexican market, for those smuggled to the USA, and for those legally exported before or after 1992, when the USA ban led parrot exports to be mostly directed to European countries. Finally, the long-term cross-cultural preference for the most attractive species has led them to be among the most threatened species today. Since current parrot poaching mostly responds to local demand, socio-ecological work is needed to reverse the long-standing pet-keeping tradition that may decimate the most desired species in Neotropical countries.

  9. Bacterial Contamination of CT Equipment: Use of ATP Detection and Culture Results to Target Quality Improvement.

    Science.gov (United States)

    Childress, John; Burch, Debborah; Kucharski, Cheryl; Young, Carol; Kazerooni, Ella A; Davenport, Matthew S

    2017-08-01

    This study aimed to evaluate the use of an adenosine triphosphate (ATP) monitoring system to minimize surface contamination on inpatient computed tomography (CT) scanners. The bore, table, and wrap of two quaternary care inpatient CT scanners (load/scanner: ~ 30-40 CT examinations/day) were assayed with bacterial cultures and an ATP detection system during six prospective iterative plan-do-check-act improvement cycles from January 6, 2016 to October 12, 2016. Per-cycle sampling was for eight consecutive weekdays. ATP detection was expressed as relative light units (RLUs) through a luciferase reaction, with >350 RLU considered contaminated per manufacturer recommendations. Culture swabs were placed into 6.5% NaCl broth, a Staphylococcus enrichment broth, and incubated aerobically at 37°C for 48 hours. Positive broths were plated to chromogenic Staphylococcus media. Culture rates (Fisher exact test) and RLU values (Mann-Whitney U test) were compared. In Cycle 1, both culture results and median RLU values indicated the wrap was the most contaminated item (positive culture rate: 63% [10/16], median RLU interquartile range: 173 [IQR: 56-640]); however, RLU values were not predictive of per-sample culture results (P = .36). Following iterative improvements, RLU values at Cycle 6 were significantly lower than at peak (P = .02-.04) and within manufacturer's recommendations: all samples: 45 (IQR: 16-87), bore: 26 (IQR: 0-51), table: 68 (IQR: 21-89), wrap: 47 (IQR: 38-121). The Velcro wrap is the most contaminated item on a CT scanner, and special processes may be needed to ensure adequate cleansing. ATP detection is a crude surrogate for bacterial culture results but benefits from speed, reduced cost, and greater statistical power. Copyright © 2017 The Association of University Radiologists. Published by Elsevier Inc. All rights reserved.

  10. 试论公共艺术在城市景观中的重要性%On Showing Cultural Characteristics of Urban Landscape by Public Art

    Institute of Scientific and Technical Information of China (English)

    罗智; 李明炅

    2012-01-01

    This paper discusses on showing cultural characteristics of urban landscape by public art, together with relative concept and cases.%公共艺术是城市景观的重要组成要素之一。通过分析公共艺术的概念和国内外公共艺术作品的实例,探讨如何在城市景观中体现公共艺术的文化性。

  11. High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis

    Directory of Open Access Journals (Sweden)

    Kristina Vukusic

    2013-01-01

    Full Text Available 3D environment and high cell density play an important role in restoring and supporting the phenotypes of cells represented in cardiac tissues. The aim of this study was therefore to investigate the suitability of high density sphere (HDS cultures for studies of cardiomyocyte-, endothelial-, and stem-cell biology. Primary adult cardiac cells from nine human biopsies were cultured using different media for up to 9 weeks. The possibilities to favor a certain cell phenotype and induce production of extra cellular matrix (ECM were studied by histology, immunohistochemistry, and quantitative real-time PCR. Defined media gave significant increase in both cardiac- and progenitor-specific markers and also an intraluminal position of endothelial cells over time. Cardiac media showed indication of differentiation and maturity of HDS considering the ECM production and activities within NOTCH regulation but no additional cardiac differentiation. Endothelial media gave no positive effects on endothelial phenotype but increased proliferation without fibroblast overgrowth. In addition, indications for early vasculogenesis were found. It was also possible to affect the Wnt signaling in HDS by addition of a glycogen synthase kinase 3 (GSK3 inhibitor. In conclusion, these findings show the suitability of HDS as in vitro model for studies of cardiomyocyte-, endothelial-, and stem-cell biology.

  12. Management Control, Results-Oriented Culture and Public Sector Performance : Empirical Evidence on New Public Management

    NARCIS (Netherlands)

    Verbeeten, Frank H M; Speklé, Roland F.

    2015-01-01

    New Public Management (NPM) has been guiding public sector reform for over 25 years. Its position on the design of effective management control rests on three key ideas: (1) performance improvement requires a results-oriented culture that emphasizes outcomes rather than inputs or processes; (2) publ

  13. Birthweight distribution in ART singletons resulting from embryo culture in two different culture media compared with the national population

    DEFF Research Database (Denmark)

    Lemmen, Josephine Gabriela; Pinborg, Anja; Rasmussen, S

    2014-01-01

    STUDY QUESTION: Is there a difference in birthweight distribution in ART singletons born after IVF culture in two different culture media? SUMMARY ANSWER: There is no effect of culture media on both crude and adjusted birthweight distributions in ART singletons from nulliparous mothers. WHAT...... IS KNOWN ALREADY: Studies on human ART singletons have reported a difference in birthweight in singletons following IVF culture in different culture media. However, other studies comparing different culture media have not shown any significant differences in birthweight. STUDY DESIGN, SIZE, DURATION...... culture media groups, we found the same slightly lower mean birthweight in IVF/ICSI singletons when compared with the national birth cohort as has been previously reported (Cook-d2 + Medicult-d2 + d3 versus birth cohort; girls: P

  14. Effect of adipose tissue processing procedures in culture result: a study preliminary

    Directory of Open Access Journals (Sweden)

    Jeanne A. Pawitan

    2011-02-01

    Full Text Available Background: There are various methods of processing adipose tissue before culture, depending on the adipose tissue samples. The aim of this study is to compare several modifications of culturing and sub-culturing procedures of adipose tissue to fit the condition in our laboratory.Method: This is a descriptive study that was done in the Immunology and Endocrinology Integrated Laboratory, University of Indonesia, from  October 2009 to April 2010. Three adipose tissue processing procedures, various amount of seeding and two subculture methods were compared in term of cell yield and time needed. In the first procedure, collagenase-1 digestion was done in 30minutes, cell seeding were 24,000 and 36,000 per flask; in the second procedure, collagenase-1 digestion was done in 60minutes, cell seeding were 24,000, 48,000, and 72,000 per flask; and in the third procedure, the adipose tissue remnants from the first  procedure were again digested for another 45 minutes, cell seeding were 74,000, and 148,000 per flask. Difference in subculture methods were the presence or absence of washing step.Result: Procedure 1 yielded the lowest amount of cell, and after culture, the cells grew very slow, and was contaminated before harvest of primary culture. Procedure-2 and -3 succeeded to yield primary cultures. Some of the cultures were contaminated, so that further subculture was not  applicable, and only one tissue processing procedure (procedure 2: 60 minute collagenase-1 digestion, without lysis buffer, cell seeding 48,000 and 72,000 could complete the three subcultures. Though some of the procedures could not be completed, final result could be concluded.Conclusion: In this preliminary study, 60 minute colagenase-1 digestion with intermittent shaking every 5 minutes and cell seeding around 50,000 or more, followed by subculture method without washing step gave the best result. (Med J Indones 2011; 20:15-9Keywords: collagenase-1, primary culture, subculture

  15. Medical educational culture: introducing patients to applicants as part of the medical school interview: feasibility and initial impact show and tell

    Directory of Open Access Journals (Sweden)

    Shireen Madani Sims

    2016-08-01

    Full Text Available Introduction: The College of Medicine at our institution underwent a major curricular revision in order to develop a patient-centered context for learning. The admission process was revised to reflect this change, adopting a holistic review process, with the hope of attracting students who were particularly well suited to a patient-centered curriculum and learning culture. Methods: Patients from a single practitioner, who were accustomed to working with medical students, were asked if they would like to select the next generation of physicians. The patient's experience included a brief didactic presentation related to the patient's diagnosis and treatment. This was followed by an informal session with the applicants and the physician, where they shared their story in a small group setting. They were encouraged to share their experiences with the healthcare system, both positive and negative. The goal was to allow applicants to glean the importance of the human aspects of disease in our institutional culture of learning. Results: The response and experience were overwhelmingly positive for the patients who donated their time to participate and for our applicants. Follow-up surveys indicated that our applicants found the experience to be unique and positive. Many of the students who chose to attend our university cited the interview experience and learning culture as factors that influenced their choice of medical schools. In addition, the Liaison Committee on Medical Education cited the favorability of the admission process in their recent site visit. Discussion: Now in its fifth year, we can say that the inclusion of patients as part of the interview day is feasible as part of our admission process. We continue to make changes and monitor our progress, and we have added several other faculty members and specialties in order to ensure the program is sustainable.

  16. Comparing results of cultured and uncultured biological methods used in biological phosphorus removal

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Increasing attention has been paid to phosphate-accumulating organisms (PAOs) for their important role in biological phosphorus removal. In this study, microbial communities of PAOs cultivated under different carbon sources (sewage, glucose, and sodium acetate) were investigated and compared through culture-dependent and culture-independent methods, respectively. The results obtained using denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction-amplified 16S rDNA fragments revealed that the diversity of bacteria in a sewage-fed reactor (1#) was much higher than in a glucose-fed one (2#) and a sodium acetate-fed one (3#); there were common PAOs in three reactors fed by different carbon sources. Five strains were separated from three systems by using a phosphate-rich medium; they were from common bacteria isolated and three isolates could not be found in DGGE profile at all. Two isolates had good phosphorus removal ability. When the microbial diversity was studied, the molecular biological method was better than the culture-dependent one. When phosphorus removal characteristics were investigated, culture-dependent approach was more effective. Thus a combination of two methods is necessary to have a comprehensive view of PAOs.

  17. O "Grande Irmão" e a empresa: indústria cultural, reality shows e espetáculos organizacionais

    Directory of Open Access Journals (Sweden)

    Marcos Goulart Castelo

    Full Text Available A motivação para este ensaio relaciona-se à busca de uma via mais direta de comunicação entre pesquisadores descontentes e participantes organizacionais pouco identificados com leituras críticas e mais interessados em render homenagem a certos produtos da indústria cultural. O objetivo do texto é revelar pontos de tangência entre programas de reality show e organizações produtivas, movimento para o qual se convida outros acadêmicos igualmente inconformados com a recorrente dificuldade de estabelecer um diálogo crítico entre teóricos e praticantes. Inicialmente, o texto discute cultura de massa, reality shows e empresas como produtos e produtores de uma representação social focada no espetáculo e na hiper-realidade, para depois ilustrar sua argumentação por meio do desenvolvimento de três facetas do problema: seleção de participantes, jogos de poder e manipulação de comportamentos. Nesse contexto, a posição de acadêmicos como meros espectadores não parece condizente com os desafios enfrentados pelos jogadores/participantes/atores dos programas e das organizações.

  18. Predicting results of mycobacterial culture on sputum smear reversion after anti-tuberculous treatment: a case control study

    Directory of Open Access Journals (Sweden)

    Lee Li-Na

    2010-03-01

    Full Text Available Abstract Background Little is currently known regarding sputum smear reversion (acid-fast smear becomes positive again after negative conversion during anti-tuberculous treatment. This study aimed to evaluate its occurrence in patients with pulmonary tuberculosis (TB and identify factors predicting results of mycobacterial culture for smear-reversion of sputum samples. Methods The retrospective review was performed in a tertiary referral center and a local teaching hospital in Taiwan. From 2000 to 2007, patients with smear-positive culture-confirmed pulmonary TB experiencing smear reversion after 14 days of anti-tuberculous treatment were identified. Results The 739 patients with smear-positive pulmonary TB had 74 (10% episodes of sputum smear reversion that grew Mycobacterium tuberculosis in 22 (30% (Mtb group. The remaining 52 episodes of culture-negative sputum samples were classified as the non-Mtb group. The anti-tuberculous regimen was modified after confirming smear reversion in 15 (20%. Fourteen episodes in the Mtb group and 15 in the non-Mtb group occurred during hospitalization. All were admitted to the negative-pressure rooms at the time of smear reversion. Statistical analysis showed that any TB drug resistance, smear reversion within the first two months of treatment or before culture conversion, and the absence of radiographic improvement before smear reversion were associated with the Mtb group. None of the smear reversion was due to viable M. tuberculosis if none of the four factors were present. Conclusions Sputum smear reversion develops in 10% of patients with smear-positive pulmonary TB, with 30% due to viable M. tuberculosis bacilli. Isolation and regimen modification may not be necessary for all drug-susceptible patients who already have radiographic improvement and develop smear reversion after two months of treatment or after sputum culture conversion.

  19. Cross-cultural aspects of ICT use by older people: preliminary results of a four-country ethnographical study

    DEFF Research Database (Denmark)

    Blat, Josep; Sayago, Sergio; Kälviäinen, Mirja

    2011-01-01

    Culture is crucial in understanding how people use technologies and designing better ones. However, very little is known about cross-cultural aspects of Information and Communication Technologies (ICT) use by older people (60+), despite the heterogeneity of this user group. This short paper...... addresses this issue by drawing on an ethnographical study of ICT use conducted with over 120 people, aged 67-71, in four European countries: Finland, Denmark, Italy and Spain, over a 6-month period. The preliminary results show that making a social, independent and worth use of ICT are common aspects...... across the four countries, despite the so-called heterogeneity of older people as ICT users. This short paper also touches on two key aspects which emerged from the study, engaging older people in research and the evolution of some barriers to technology use....

  20. Show Time

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    <正> Story: Show Time!The whole class presents the story"Under the Sea".Everyone is so excited and happy.Both Leo and Kathy show their parentsthe characters of the play."Who’s he?"asks Kathy’s mom."He’s the prince."Kathy replies."Who’s she?"asks Leo’s dad."She’s the queen."Leo replieswith a smile.

  1. Snobbish Show

    Institute of Scientific and Technical Information of China (English)

    YIN PUMIN

    2010-01-01

    @@ The State Administration of Radio,Film and Television (SARFT),China's media watchdog,issued a new set of mles on June 9 that strictly regulate TV match-making shows,which have been sweeping the country's primetime programming. "Improper social and love values such as money worship should not be presented in these shows.Humiliation,verbal attacks and sex-implied vulgar content are not allowed" the new roles said.

  2. Transitioning from preclinical to clinical chemopreventive assessments of lyophilized black raspberries: interim results show berries modulate markers of oxidative stress in Barrett's esophagus patients.

    Science.gov (United States)

    Kresty, Laura A; Frankel, Wendy L; Hammond, Cynthia D; Baird, Maureen E; Mele, Jennifer M; Stoner, Gary D; Fromkes, John J

    2006-01-01

    Increased fruit and vegetable consumption is associated with decreased risk of a number of cancers of epithelial origin, including esophageal cancer. Dietary administration of lyophilized black raspberries (LBRs) has significantly inhibited chemically induced oral, esophageal, and colon carcinogenesis in animal models. Likewise, berry extracts added to cell cultures significantly inhibited cancer-associated processes. Positive results in preclinical studies have supported further investigation of berries and berry extracts in high-risk human cohorts, including patients with existing premalignancy or patients at risk for cancer recurrence. We are currently conducting a 6-mo chemopreventive pilot study administering 32 or 45 g (female and male, respectively) of LBRs to patients with Barrett's esophagus (BE), a premalignant esophageal condition in which the normal stratified squamous epithelium changes to a metaplastic columnar-lined epithelium. BE's importance lies in the fact that it confers a 30- to 40-fold increased risk for the development of esophageal adenocarcinoma, a rapidly increasing and extremely deadly malignancy. This is a report on interim findings from 10 patients. To date, the results support that daily consumption of LBRs promotes reductions in the urinary excretion of two markers of oxidative stress, 8-epi-prostaglandin F2alpha (8-Iso-PGF2) and, to a lesser more-variable extent, 8-hydroxy-2'-deoxyguanosine (8-OHdG), among patients with BE.

  3. EROBATIC SHOW

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    Visitors look at plane models of the Commercial Aircraft Corp. of China, developer of the count,s first homegrown large passenger jet C919, during the Singapore Airshow on February 16. The biennial event is the largest airshow in Asia and one of the most important aviation and defense shows worldwide. A number of Chinese companies took part in the event during which Okay Airways, the first privately owned aidine in China, signed a deal to acquire 12 Boeing 737 jets.

  4. Comparative usefulness of inflammatory markers to indicate bacterial infection-analyzed according to blood culture results and related clinical factors.

    Science.gov (United States)

    Nishikawa, Hirokazu; Shirano, Michinori; Kasamatsu, Yu; Morimura, Ayumi; Iida, Ko; Kishi, Tomomi; Goto, Tetsushi; Okamoto, Saki; Ehara, Eiji

    2016-01-01

    To assess relationships of inflammatory markers and 2 related clinical factors with blood culture results, we retrospectively investigated inpatients' blood culture and blood chemistry findings that were recorded from January to December 2014 using electronic medical records and analyzed the data of 852 subjects (426 culture-positive and 426 culture-negative). Results suggested that the risk of positive blood culture statistically increased as inflammatory marker levels and the number of related factors increased. Concerning the effectiveness of inflammatory markers, when the outcome definition was also changed for C-reactive protein (CRP), the odds ratio had a similar value, whereas when the outcome definition of blood culture positivity was used for procalcitonin (PCT), the greatest effectiveness of that was detected. Therefore, the current results suggest that PCT is more useful than CRP as an auxiliary indication of bacterial infection.

  5. Heavy metal uptake in foraminiferal calcite: results of multi-element culture experiments

    Directory of Open Access Journals (Sweden)

    D. Munsel

    2010-02-01

    Full Text Available The incorporation of heavy metals into the test of the shallow water benthic foraminifer Ammonia tepida was investigated under controlled laboratory conditions. Except for the concentrations of the trace elements, all other culture conditions such as pH, temperature and salinity were kept constant. In the experiments, the concentrations of Ni, Cu and Mn were 5, 10, and 20 times higher than those in natural North Sea water, whereas in a control experiment foraminifera were cultured in filtered natural North Sea water. Concentrations of Cu and Ni from newly grown chambers were determined by means of both μ-synchrotron XRF and laser ablation inductively coupled plasma mass spectroscopy (LA-ICP-MS. Both independent analytical approaches agreed within the analytical uncertainty intervals. The calculated partition coefficients were 0.17±0.09 and 1.3±0.7 for Cu and Ni, respectively. Potential toxic and/or chemical competition effects might have lead to a decreasing incorporation rate of Cu and Ni into the calcite of the specimens of the tank with the highest chemical concentrations. Mn showed great scattering in the aquarium with the 20-fold higher element concentrations potentially due to antagonism effects with Cu. Nevertheless, the established partition coefficients now open the way for reconstructing past concentrations for these elements in sea water.

  6. The uneven results of institutional changes in Central and Eastern Europe: The role of culture

    Directory of Open Access Journals (Sweden)

    Pejović Svetozar

    2004-01-01

    Full Text Available It has been widely observed that the same formal rules, enacted in the parliaments in the form of written laws, give vastly different results in different social and cultural environments. This phenomenon came to be particularly pronounced in the process of transition of the formerly communist countries to market economies and politically pluralized societies. Highly similar and occasionally identical institutional changes turned out to be unequally accepted by the societies under consideration and produced widely different results in the material restructuring of the economies. It became clear that the notion of institutions had to be widened so as to encompass the informal rules: the customs, the traditions, cultural values and national myths. Informal rules define the constraints for implementing the formal ones and, on the other hand, determine the actual effects of the latter once they are implemented. Forcing the formal rules upon the transition societies cannot be successful unless preceding and/or contemporaneous changes of informal rules are provided for. The paper ends with a design of the strategy for the decisively important changes in values and other components of informal rules.

  7. Nissan Showcases the Results of an Energy-Wise Corporate Culture

    Energy Technology Data Exchange (ETDEWEB)

    None

    2010-06-11

    The corporate leadership at Nissan cultivates a culture of energy efficiency, encouraging employees to practice good energy management at work and in every part of their lives. Read about Nissan's energy-conscience culture.

  8. Evaluation of patient safety culture among Malaysian retail pharmacists: results of a self-reported survey

    Directory of Open Access Journals (Sweden)

    Sivanandy P

    2016-07-01

    Full Text Available Palanisamy Sivanandy,1 Mari Kannan Maharajan,1 Kingston Rajiah,1 Tan Tyng Wei,2 Tan Wee Loon,2 Lim Chong Yee2 1Department of Pharmacy Practice, School of Pharmacy, 2School of Pharmacy, International Medical University, Wilayah Persekutuan Kuala Lumpur, Malaysia Background: Patient safety is a major public health issue, and the knowledge, skills, and experience of health professionals are very much essential for improving patient safety. Patient safety and medication error are very much associated. Pharmacists play a significant role in patient safety. The function of pharmacists in the medication use process is very different from medical and nursing colleagues. Medication dispensing accuracy is a vital element to ensure the safety and quality of medication use.Objective: To evaluate the attitude and perception of the pharmacist toward patient safety in retail pharmacies setup in Malaysia.Methods: A Pharmacy Survey on Patient Safety Culture questionnaire was used to assess patient safety culture, developed by the Agency for Healthcare Research and Quality, and the convenience sampling method was adopted.Results: The overall positive response rate ranged from 31.20% to 87.43%, and the average positive response rate was found to be 67%. Among all the eleven domains pertaining to patient safety culture, the scores of “staff training and skills” were less. Communication openness, and patient counseling are common, but not practiced regularly in the Malaysian retail pharmacy setup compared with those in USA. The overall perception of patient safety of an acceptable level in the current retail pharmacy setup.Conclusion: The study revealed that staff training, skills, communication in patient counseling, and communication across shifts and about mistakes are less in current retail pharmacy setup. The overall perception of patient safety should be improved by educating the pharmacists about the significance and essential of patient safety. Keywords

  9. Microbiology of shallow subsurface aquifer and carbonate rocks studied by culture-dependent and culture-independent methods. Preliminary results on an underground laboratory, the LSBB, Rustrel, France

    Directory of Open Access Journals (Sweden)

    Galès Grégoire

    2016-01-01

    Full Text Available We investigated the microbiology of a shallow subsurface site, the LSBB, located near Avignon. This site lies in carbonate rocks, belonging to the Urgonian facies. Rock, concrete and water samples were collected and directly transferred to the laboratory. Studies of microorganisms as pure cultures are the only way to get their real physiological properties. Nevertheless, microbiologists cannot cultivate and isolate the majority of microorganisms for several reasons, one being our lack of understanding of their minimal needs. Molecular studies, e.g. extraction and sequencing of the total nucleic acids present in an environment provide phylogenetic and metabolic information on uncultivated microorganisms. We performed aerobic and anaerobic culture with various electron acceptors and donors, searching for heterotrophic, methanogenic, sulphate- nitrate- and FeIII- reducing Prokaryotes. We also performed DNA extractions and PCR amplification of ribosomal RNA genes, to test if our protocols were adapted to this environment. Our results show that the LSBB galleries are colonized by a low diversity microbiote, with a strong influence of anthropogenic activities. Further studies will link the microorganisms biodiversity and the petrophysic properties of rocks.

  10. Coccidioidomycosis: adenosine deaminase levels, serologic parameters, culture results, and polymerase chain reaction testing in pleural fluid.

    Science.gov (United States)

    Thompson, George R; Sharma, Shobha; Bays, Derek J; Pruitt, Rachel; Engelthaler, David M; Bowers, Jolene; Driebe, Elizabeth M; Davis, Michael; Libke, Robert; Cohen, Stuart H; Pappagianis, Demosthenes

    2013-03-01

    In a patient with positive serum serology for coccidioidomycosis, the differential diagnosis of concurrent pleural effusions can be challenging. We, therefore, sought to clarify the performance characteristics of biochemical, serologic, and nucleic-acid-based testing in an attempt to avoid invasive procedures. The utility of adenosine deaminase (ADA), coccidioidal serology, and polymerase chain reaction (PCR) in the evaluation of pleuropulmonary coccidioidomycosis has not been previously reported. Forty consecutive patients evaluated for pleuropulmonary coccidioidomycosis were included. Demographic data, pleural fluid values, culture results, and clinical diagnoses were obtained from patient chart review. ADA testing was performed by ARUP Laboratories, coccidioidal serologic testing was performed by the University of California-Davis coccidioidomycosis serology laboratory, and PCR testing was performed by the Translational Genomics Research Institute using a previously published methodology. Fifteen patients were diagnosed with pleuropulmonary coccidioidomycosis by European Organization for the Research and Treatment of Cancer/Mycoses Study Group criteria. Pleural fluid ADA concentrations were coccidioidomycosis were not elevated in this study. The sensitivity and specificity of coccidioidal serologic testing in nonserum samples remained high, but the clinical usefulness of PCR testing in pleural fluid was disappointing and was comparable to pleural fluid culture.

  11. 服装表演传播的商业和文化功能分析%Commercial and Cultural Functions of Fashion Show Transmission

    Institute of Scientific and Technical Information of China (English)

    侯锐

    2013-01-01

    This paper describes the transmission mode of the fashion show, points that the different types of performances need different mode of transmission, and focuses on business and social functions of the fashion show. The fashion show is a kind of communication activities, but its nature is the transfer of information. This paper analyzes the function and results of transmission, and explores the fashion show with several established theoretical model and points out the positive and negative social function of the fashion show.%  文章阐述了服装表演的传播模式,指出不同类型的表演需要选择不同的传播模式,着重讨论了服装表演的商业和社会功能。服装表演是一种传播活动,但就其本质是信息的传递。提出了针对传播的功能和传播效果的分析,并用几个既定的理论模型来分析服装表演。指出服装表演的积极和消极社会功能。

  12. Use of a serum-free epidermal culture model to show deleterious effects of epidermal growth factor on morphogenesis and differentiation.

    Science.gov (United States)

    Chen, C S; Lavker, R M; Rodeck, U; Risse, B; Jensen, P J

    1995-01-01

    The presence of serum has limited the utility of many culture models for the study of cytokine effects because its complexity and variability can confound the interpretation of data. In the present study, a serum-free skin co-culture model was used to investigate the effect of exogenous epidermal growth factor (EGF) on epidermal proliferation and differentiation. Human keratinocytes cultured on collagen rafts at the air-liquid interface produced a well-differentiated epithelium that resembled normal epidermis. Keratin filaments, membrane-coating granules, and keratohyalin granules were all observed. Epidermal differentiation markers keratin K1/K10, involucrin, and transglutaminase were localized in most of the suprabasal layers, whereas profilaggrin/filaggrin was confined to the granular layers and stratum corneum. In the continual presence of 10-20 ng/mL EGF, the epidermis was less organized, thinner, and less proliferative. EGF also depressed several indicators of differentiation: The number of keratohyalin granules and membrane-coating granules was greatly decreased; antigen expression of profilaggrin/filaggrin appeared diminished by immunocytochemical staining; frequent nuclear retention was noted in the relatively thickened stratum corneum-like layers. As detected by immunohistochemical staining, the expression of EGF receptor in the epidermis was reduced by exogenous EGF. These data illustrate that EGF cannot be considered a simple mitogen. Our findings also underscore the importance of using sophisticated culture models to assess complex cytokine effects that may be dependent on the architecture of a differentiating epidermis.

  13. Usability in a Cultural Context: A Report on the Scope, Process and Research Results of CultUsab - The Cultural Usability Project

    OpenAIRE

    Clemmensen, Torkil; Yammiyavar, Pradeep; Ørngreen, Rikke; Katre, Dinesh

    2010-01-01

    International audience; This paper focuses on presenting and discussing the aim, context, challenges, results, and impact of the Cultural usability project named as CultUsab. This project was a four year international research effort from 2006 to 2009, which was supported by a grant from the Danish Research Councils for Independent Research in Culture and Communication. The project aimed at innovating processes in Information and Communication Technology development through an understanding o...

  14. 237例血培养阳性结果分析%Analysis of 237 positive results from blood cultures

    Institute of Scientific and Technical Information of China (English)

    陈梅莲; 邓任堂; 李柳燕; 李金洳; 付文金

    2015-01-01

    Objective To investigate the strains distribution and resistance characteristics of pathogenic bacteria isolated from blood culture and provide basis for clinical medication. Methods Blood samples were cultivated and continuously monitored by VersaTREK automated blood culture system with the matching blood culture bottles,and the positive bottles were transferred timely. The identification of bacteria and susceptibility test were performed by MicroScan A/S-4 bacterial automatic analyzer. Results A total of 237 strains of pathogenic bacteria were isolated from 2460 blood culture samples. The top five strains included CNS, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Enterococcus. Positive pathogens were mainly distributed in ICU, general surgical department and respiratory medicine department. Susceptibility tests showed that Gram negative bacilli were highly sensitive to imipenem and Gram positive cocci had no resistance to vancomycin and linezolid. Conclusion It is important to comprehend the blood culture results promptly for the selection of antimicrobial agents and has great significance to improve cure rate.%目的:对血培养病原菌的种类分布和耐药情况进行回顾性分析,为临床用药提供依据。方法采用VersaTREK全自动血培养仪及配套血培养瓶连续监测培养细菌,阳性瓶及时转种。用MicroScan A/S-4微生物分析仪及配套试剂对分离菌进行鉴定和药敏试验。结果2460例血培养标本中共分离出237株病原菌,居前5位的病原菌依次为凝固酶阴性葡萄球菌、大肠埃希菌、肺炎克雷伯菌、金黄色葡萄球菌和肠球菌。临床分布以ICU、普外科及呼吸内科为主。药敏结果显示:革兰阴性杆菌对亚胺培南高度敏感,未发现耐万古霉素和利奈唑胺的革兰阳性球菌。结论及时了解血培养结果可以为临床抗菌治疗提供依据,对提高治愈率有重要的意义。

  15. Comparison of length of stay and outcomes of patients with positive versus negative blood culture results.

    Science.gov (United States)

    Armstrong-Briley, Danielle; Hozhabri, Neda S T; Armstrong, Kris; Puthottile, Jason; Benavides, Raul; Beal, Stacy

    2015-01-01

    In the United States, sepsis is the leading cause of death in critically ill patients. The fatality rate for severe sepsis is about 40%, and treatment costs over $16 billion annually. It is critical to identify and treat the source of sepsis. While there are varying guidelines determining when to draw blood for culture, at Baylor University Medical Center at Dallas, blood cultures are ordered for patients with new onset of fever, immunosuppression, or a suspicion of an underlying infectious etiology. We conducted a retrospective study of patients who had blood cultures after hospital admission or in the emergency department in December 2013. We compared length of stay and outcomes of patients with positive versus negative blood cultures. There was no significant difference for length of stay or outcomes among patients with positive and negative blood cultures. For patients admitted from the emergency department, there was a longer length of stay for patients with positive cultures; however, the overall prognosis was not worse.

  16. Do students have cultural scripts? Results from the first implementation of open source tutorials in Japan

    Science.gov (United States)

    Hull, Michael Malvern

    In the 1980's and 1990's, results from flurries of standardized exams (particularly in 4th and 8th grade mathematics and science) reached the attention of ever-growing numbers of Americans with an alarming message: our children are not even close to keeping up with those in China, Japan, and Korea. As a step towards improving American classrooms, cross-cultural education researchers began to investigate differences in classroom structure, curricular content and focus, and attitudes and beliefs of students towards learning. Based upon research findings in cognitive science about the fluidity of student beliefs, we hypothesized that the treatment of student beliefs as being stable is overly simplistic and ineffective at describing certain classroom phenomena that would be of interest to the cross-cultural education research field. We felt such phenomena could be instructional to American educators, and that a failure to understand such phenomena would imply a failure to learn from these classrooms. We hypothesized that, were we to introduce reformed physics curriculum from America to students in Japan, we would observe context-dependency in how students approached the material. Furthermore, we hypothesized that this curriculum, which was motivated by the assumption that students have multiple ways of approaching knowing and learning, would be productive in the Japanese classroom. Either of these results would go against the tacit assumption of the cross-cultural education research field that students have a stable belief about how learning should take place, and would cast doubt on such a framework. Curriculum developed and tested at the University of Maryland was translated into Japanese and implemented in the spring semester of 2011 at Tokyo Gakugei University. Based upon available literature on the education system in Japan, we hypothesized that students would be entering the college classroom having had three years of cramming for entrance exams in high school

  17. [Pediatric osteo-articular infections with negative culture results: what about Kingella kingae?].

    Science.gov (United States)

    Cherkaoui, A; Ceroni, D; Ferey, S; Emonet, S; Schrenzel, J

    2009-11-04

    Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no crossreactivity with several related species and common osteoarticular pathogens. Its clinical impact is illustrated by three pediatric cases.

  18. Pilot outcome results of culturally adapted evidence-based substance use disorder treatment with a Southwest Tribe

    Directory of Open Access Journals (Sweden)

    Kamilla L. Venner

    2016-06-01

    Conclusions: Results suggest that culturally adapted EBTs yield significant improvements in alcohol use, psychological distress, and legal problems among AI/ANs. Future research using RCT methodology is needed to examine efficacy and effectiveness.

  19. Analysis of conservative tracer measurement results using the Frechet distribution at planted horizontal subsurface flow constructed wetlands filled with coarse gravel and showing the effect of clogging processes.

    Science.gov (United States)

    Dittrich, Ernő; Klincsik, Mihály

    2015-11-01

    A mathematical process, developed in Maple environment, has been successful in decreasing the error of measurement results and in the precise calculation of the moments of corrected tracer functions. It was proved that with this process, the measured tracer results of horizontal subsurface flow constructed wetlands filled with coarse gravel (HSFCW-C) can be fitted more accurately than with the conventionally used distribution functions (Gaussian, Lognormal, Fick (Inverse Gaussian) and Gamma). This statement is true only for the planted HSFCW-Cs. The analysis of unplanted HSFCW-Cs needs more research. The result of the analysis shows that the conventional solutions (completely stirred series tank reactor (CSTR) model and convection-dispersion transport (CDT) model) cannot describe these types of transport processes with sufficient accuracy. These outcomes can help in developing better process descriptions of very difficult transport processes in HSFCW-Cs. Furthermore, a new mathematical process can be developed for the calculation of real hydraulic residence time (HRT) and dispersion coefficient values. The presented method can be generalized to other kinds of hydraulic environments.

  20. On the design principle of modern science and technology culture show%浅析现代科技文化展示的设计原则

    Institute of Scientific and Technical Information of China (English)

    钱涛

    2014-01-01

    在信息化的今天,科技文化展示设计与数字技术发展有着天然的联系,形成不同与其它展示设计的内容与性质,因此,传统的展示原则与现代科技展示设计的发展已不相适应,本文力图从通用性、易用性和快乐性等设计原则的探析,实现科技文化展示设计中艺术与科技的完美统一。%In today's information age, science and technology culture exhibition design with the development of digital technology has a natural connection, unlike other display design content and nature, form the therefore, display principle of traditional and modern technology exhibition design is not adapt to the development, this paper tries to universal, easy to use and so on happiness the design principle, implementation science and technology culture display the perfect unity of art and technology in design.

  1. Transgene silencing of the Hutchinson-Gilford progeria syndrome mutation results in a reversible bone phenotype, whereas resveratrol treatment does not show overall beneficial effects

    DEFF Research Database (Denmark)

    Strandgren, Charlotte; Nasser, Hasina Abdul; McKenna, Tomás

    2015-01-01

    Hutchinson-Gilford progeria syndrome (HGPS) is a rare premature aging disorder that is most commonly caused by a de novo point mutation in exon 11 of the LMNA gene, c.1824C>T, which results in an increased production of a truncated form of lamin A known as progerin. In this study, we used a mouse...... model to study the possibility of recovering from HGPS bone disease upon silencing of the HGPS mutation, and the potential benefits from treatment with resveratrol. We show that complete silencing of the transgenic expression of progerin normalized bone morphology and mineralization already after 7...... weeks. The improvements included lower frequencies of rib fractures and callus formation, an increased number of osteocytes in remodeled bone, and normalized dentinogenesis. The beneficial effects from resveratrol treatment were less significant and to a large extent similar to mice treated with sucrose...

  2. Simple instruments used in monitoring ionospheric perturbations and some observational results showing the ionospheric responses to the perturbations mainly from the lower atmosphere

    Science.gov (United States)

    Xiao, Zuo; Hao, Yongqiang; Zhang, Donghe; Xiao, Sai-Guan; Huang, Weiquan

    Ionospheric disturbances such as SID and acoustic gravity waves in different scales are well known and commonly discussed topics. Some simple ground equipment was designed and used for monitoring continuously the effects of these disturbances, especially, SWF, SFD. Besides SIDs, They also reflect clearly the acoustic gravity waves in different scale and Spread-F and these data are important supplementary to the traditional ionosonde records. It is of signifi-cance in understanding physical essentials of the ionospheric disturbances and applications in SID warning. In this paper, the designing of the instruments is given and results are discussed in detail. Some case studies were introduced as example which showed very clearly not only immediate effects of solar flare, but also the phenomena of ionospheric responses to large scale gravity waves from lower atmosphere such as typhoon, great earthquake and volcano erup-tion. Particularlyresults showed that acoustic gravity waves play significant role in seeding ionospheric Spread-F. These examples give evidence that lower atmospheric activities strongly influence the ionosphere.

  3. Transgene silencing of the Hutchinson-Gilford progeria syndrome mutation results in a reversible bone phenotype, whereas resveratrol treatment does not show overall beneficial effects.

    Science.gov (United States)

    Strandgren, Charlotte; Nasser, Hasina Abdul; McKenna, Tomás; Koskela, Antti; Tuukkanen, Juha; Ohlsson, Claes; Rozell, Björn; Eriksson, Maria

    2015-08-01

    Hutchinson-Gilford progeria syndrome (HGPS) is a rare premature aging disorder that is most commonly caused by a de novo point mutation in exon 11 of the LMNA gene, c.1824C>T, which results in an increased production of a truncated form of lamin A known as progerin. In this study, we used a mouse model to study the possibility of recovering from HGPS bone disease upon silencing of the HGPS mutation, and the potential benefits from treatment with resveratrol. We show that complete silencing of the transgenic expression of progerin normalized bone morphology and mineralization already after 7 weeks. The improvements included lower frequencies of rib fractures and callus formation, an increased number of osteocytes in remodeled bone, and normalized dentinogenesis. The beneficial effects from resveratrol treatment were less significant and to a large extent similar to mice treated with sucrose alone. However, the reversal of the dental phenotype of overgrown and laterally displaced lower incisors in HGPS mice could be attributed to resveratrol. Our results indicate that the HGPS bone defects were reversible upon suppressed transgenic expression and suggest that treatments targeting aberrant progerin splicing give hope to patients who are affected by HGPS.

  4. Melanoma cultures show different susceptibility towards E1A-, E1B-19 kDa- and fiber-modified replication-competent adenoviruses.

    Science.gov (United States)

    Schmitz, M; Graf, C; Gut, T; Sirena, D; Peter, I; Dummer, R; Greber, U F; Hemmi, S

    2006-06-01

    Replicating adenovirus (Ad) vectors with tumour tissue specificity hold great promise for treatment of cancer. We have recently constructed a conditionally replicating Ad5 AdDeltaEP-TETP inducing tumour regression in a xenograft mouse model. For further improvement of this vector, we introduced four genetic modifications and analysed the viral cytotoxicity in a large panel of melanoma cell lines and patient-derived melanoma cells. (1) The antiapoptotic gene E1B-19 kDa (Delta19 mutant) was deleted increasing the cytolytic activity in 18 of 21 melanoma cells. (2) Introduction of the E1A 122-129 deletion (Delta24 mutant), suggested to attenuate viral replication in cell cycle-arrested cells, did not abrogate this activity and increased the cytolytic activity in two of 21 melanoma cells. (3) We inserted an RGD sequence into the fiber to extend viral tropism to alphav integrin-expressing cells, and (4) swapped the fiber with the Ad35 fiber (F35) enhancing the tropism to malignant melanoma cells expressing CD46. The RGD-fiber modification strongly increased cytolysis in all of the 11 CAR-low melanoma cells. The F35 fiber-chimeric vector boosted the cytotoxicity in nine of 11 cells. Our results show that rational engineering additively enhances the cytolytic potential of Ad vectors, a prerequisite for the development of patient-customized viral therapies.

  5. Clinical characteristics associated with Borrelia burgdorferi sensu lato skin culture results in patients with erythema migrans.

    Directory of Open Access Journals (Sweden)

    Franc Strle

    Full Text Available Clinical characteristics associated with isolation of Borrelia burgdorferi sensu lato from skin have not been fully evaluated. To gain insight into predictors for a positive EM skin culture, we compared basic demographic, epidemiologic, and clinical data in 608 culture-proven and 501 culture-negative adult patients with solitary EM. A positive Borrelia spp. skin culture was associated with older age, a time interval of >2 days between tick bite and onset of the skin lesion, EM ≥ 5 cm in diameter, and location of the lesion on the extremities, whereas several other characteristics used as clinical case definition criteria for the diagnosis of EM (such as tick bite at the site of later EM, information on expansion of the skin lesion, central clearing were not. A patient with a 15-cm EM lesion had almost 3-fold greater odds for a positive skin culture than patients with a 5-cm lesion. Patients with a free time interval between the tick bite and onset of EM had the same probability of a positive skin culture as those who did not recall a tick bite (OR=1.02; however, the two groups had >3-fold greater odds for EM positivity than patients who reported a tick bite with no interval between the bite and onset of the lesion. In conclusion, several yet not all clinical characteristics used in EM case definitions were associated with positive Borrelia spp. skin culture. The findings are limited to European patients with solitary EM caused predominantly by B. afzelii but may not be valid for other situations.

  6. Rapamycin and chloroquine: the in vitro and in vivo effects of autophagy-modifying drugs show promising results in valosin containing protein multisystem proteinopathy.

    Directory of Open Access Journals (Sweden)

    Angèle Nalbandian

    Full Text Available Mutations in the valosin containing protein (VCP gene cause hereditary Inclusion body myopathy (hIBM associated with Paget disease of bone (PDB, frontotemporal dementia (FTD, more recently termed multisystem proteinopathy (MSP. Affected individuals exhibit scapular winging and die from progressive muscle weakness, and cardiac and respiratory failure, typically in their 40s to 50s. Histologically, patients show the presence of rimmed vacuoles and TAR DNA-binding protein 43 (TDP-43-positive large ubiquitinated inclusion bodies in the muscles. We have generated a VCPR155H/+ mouse model which recapitulates the disease phenotype and impaired autophagy typically observed in patients with VCP disease. Autophagy-modifying agents, such as rapamycin and chloroquine, at pharmacological doses have previously shown to alter the autophagic flux. Herein, we report results of administration of rapamycin, a specific inhibitor of the mechanistic target of rapamycin (mTOR signaling pathway, and chloroquine, a lysosomal inhibitor which reverses autophagy by accumulating in lysosomes, responsible for blocking autophagy in 20-month old VCPR155H/+ mice. Rapamycin-treated mice demonstrated significant improvement in muscle performance, quadriceps histological analysis, and rescue of ubiquitin, and TDP-43 pathology and defective autophagy as indicated by decreased protein expression levels of LC3-I/II, p62/SQSTM1, optineurin and inhibiting the mTORC1 substrates. Conversely, chloroquine-treated VCPR155H/+ mice revealed progressive muscle weakness, cytoplasmic accumulation of TDP-43, ubiquitin-positive inclusion bodies and increased LC3-I/II, p62/SQSTM1, and optineurin expression levels. Our in vitro patient myoblasts studies treated with rapamycin demonstrated an overall improvement in the autophagy markers. Targeting the mTOR pathway ameliorates an increasing list of disorders, and these findings suggest that VCP disease and related neurodegenerative multisystem

  7. Childhood Context Explains Cultural Variance in Implicit Parenting Motivation: Results from Two Studies with Six Samples from Cameroon, Costa Rica, Germany, and PR China

    Directory of Open Access Journals (Sweden)

    Athanasios Chasiotis

    2014-04-01

    Full Text Available We investigated the effect of the childhood context variables number of siblings (study 1 and 2 and parental SES (study 2 on implicit parenting motivation across six cultural samples, including Africa (2xCameroon, Asia (PR China, Europe (2xGermany, and Latin America (Costa Rica. Implicit parenting motivation was assessed using an instrument measuring implicit motives (OMT, Operant Multimotive Test; Kuhl and Scheffer, 2001. Replicating and extending results from previous studies, regression analyses and structural equation models show that the number of siblings and parental SES explain a large amount of cultural variance, ranging from 64% to 82% of the cultural variance observed in implicit parenting motivation. Results are discussed within the framework of evolutionary developmental psychology.

  8. Effect of Changing Governance System: Result of Western Style Management Adoption to Japanese Culture of Ambiguity

    Directory of Open Access Journals (Sweden)

    Kohichiro Hotta

    2009-02-01

    Full Text Available This paper considers the difficulty of management style change through observation of the management style of Company-A, one of the biggest Japanese IT companies. Japanese economy grew after World War II until the early 1990's. During that era, Ba or SECI process worked in Japanese organizations very well. Further, there was an ambiguous culture in the background of such characteristics. Some kinds of ambiguity or adhocracy made positive effects for Japanese organizational activity, or ambiguity played an important role for Ba activity. There were nested Ba's in each organization with ambiguity. Ambiguous descriptions of roles for each organizational unit activated nested Ba's and generated hot groups. After the economic crisis, Company-A changed its governance and gave clear targets for each organizational unit and for each employee. This change gave new difficulty and diminishes its competence. The change denied the ambiguity in the organization but it was the basis of the competence. Adopting a new system of governance is not a simple activity. Systems must be adjusted to the culture of the organization. Company-A should study competitors in different cultures and adjust the methodology for its culture.

  9. VIABILITY AND ISOLATION OF MARINE-BACTERIA BY DILUTION CULTURE - THEORY, PROCEDURES, AND INITIAL RESULTS

    NARCIS (Netherlands)

    BUTTON, DK; SCHUT, F; QUANG, P; MARTIN, R; ROBERTSON, BR

    1993-01-01

    Dilution culture, a method for growing the typical small bacteria from natural aquatic assemblages, has been developed. Each of 11 experimental trials of the technique was successful. Populations are measured, diluted to a small and known number of cells, inoculated into unamended sterilized seawate

  10. Goal Translation: How To Create a Results-Focused Organizational Culture.

    Science.gov (United States)

    Mourier, Pierre

    2000-01-01

    Presents a model for changing human and organizational behavior. Highlights include behavioral dynamics; expectations; alignment; organizational structure; organizational culture; individual skills and training; leadership; management systems; developing corporate-level goals; communicating goals to the organization; and developing employee goals.…

  11. Effect of Changing Governance System: Result of Western Style Management Adoption to Japanese Culture of Ambiguity

    Directory of Open Access Journals (Sweden)

    Kohichiro Hotta

    2009-02-01

    Full Text Available This paper considers the difficulty of management style change through observation of the management style of Company-A, one of the biggest Japanese IT companies. Japanese economy grew after World War II until the early 1990's. During that era, Ba or SECI process worked in Japanese organizations very well. Further, there was an ambiguous culture in the background of such characteristics. Some kinds of ambiguity or adhocracy made positive effects for Japanese organizational activity, or ambiguity played an important role for Ba activity. There were nested Ba's in each organization with ambiguity. Ambiguous descriptions of roles for each organizational unit activated nested Ba's and generated hot groups. After the economic crisis, Company-A changed its governance and gave clear targets for each organizational unit and for each employee. This change gave new difficulty and diminishes its competence. The change denied the ambiguity in the organization but it was the basis of the competence. Adopting a new system of governance is not a simple activity. Systems must be adjusted to the culture of the organization. Company-A should study competitors in different cultures and adjust the methodology for its culture.

  12. Chalearn looking at people 2015: Apparent age and cultural event recognition datasets and results

    DEFF Research Database (Denmark)

    Escalera, Sergio; Fabian, Junior; Baro, Xavier;

    2015-01-01

    Following previous series on Looking at People (LAP) competitions [14, 13, 11, 12, 2], in 2015 ChaLearn ran two new competitions within the field of Looking at People: (1) age estimation, and (2) cultural event recognition, both in still images. We developed a crowd-sourcing application to collec...

  13. cultural

    Directory of Open Access Journals (Sweden)

    Irene Kreutz

    2006-01-01

    Full Text Available Es un estudio cualitativo que adoptó como referencial teorico-motodológico la antropología y la etnografía. Presenta las experiencias vivenciadas por mujeres de una comunidad en el proceso salud-enfermedad, con el objetivo de comprender los determinantes sócio-culturales e históricos de las prácticas de prevención y tratamiento adoptados por el grupo cultural por medio de la entrevista semi-estructurada. Los temas que emergieron fueron: la relación entre la alimentación y lo proceso salud-enfermedad, las relaciones con el sistema de salud oficial y el proceso salud-enfermedad y lo sobrenatural. Los dados revelaron que los moradores de la comunidad investigada tienen un modo particular de explicar sus procedimientos terapéuticos. Consideramos que es papel de los profesionales de la salud en sus prácticas, la adopción de abordajes o enfoques que consideren al individuo en su dimensión sócio-cultural e histórica, considerando la enorme diversidad cultural en nuestro país.

  14. A Successfully Treated Case of Gastrointestinal Stromal Tumor Causing Severe Anemia and Localized Peritonitis Showing Angina Pectoris Resulting in Watershed Cerebral Infarction

    Directory of Open Access Journals (Sweden)

    Yoshihide Sehara

    2017-01-01

    Full Text Available Ischemic stroke following acute myocardial infarction is a rare but a serious complication. Because the pathophysiology of stroke is dynamic, it is often hard to identify the cause of stroke. Here, we present the case of a 75-year-old man with ischemic stroke following angina pectoris caused by severe anemia and localized peritonitis due to gastrointestinal stromal tumor of small intestine. On admission, he showed consciousness disturbance, fever, and left hemiplegia. The electrocardiogram on admission showed ST-segment depression in V2 to V6 which was normalized 4 hours later. The ultrasound cardiogram showed the mild hypokinesis in the apical portion of left ventricle which was also normalized later. The magnetic resonance imaging and angiography showed ischemic stroke in watershed area between right anterior and middle cerebral arteries area and stenosis of distal portion of right middle cerebral artery. The computed tomography of abdomen showed a mass of small intestine. We decided to perform curative surgery after transfusion and successfully resected the mass of the small intestine, which was revealed to be a gastrointestinal stromal tumor (GIST. This is a successfully treated case of GIST in which the complicated pathophysiology of watershed cerebral infarction following angina pectoris might be clearly revealed.

  15. Comparison of a quantitative real-time polymerase chain reaction (qPCR) with conventional PCR, bacterial culture and ELISA for detection of Mycobacterium avium subsp. paratuberculosis infection in sheep showing pathology of Johne's disease.

    Science.gov (United States)

    Sonawane, Ganesh G; Tripathi, Bhupendra N

    2013-12-01

    A quantitative real-time PCR (qPCR) assay employing IS900 gene specific primers of Mycobacterium avium subsp. parartuberculosis (MAP) was compared with conventional PCR, bacterial culture and enzyme-linked immunosorbent assay in 38 sheep showing granulomatous enteritis and lymphadenitis with and without demonstration of acid-fast bacilli (AFB). The lesions were classified as multibacillary (MB) (n = 23), which had diffuse granulomatous lesions with abundant AFB, and paucibacillary (PB) (n = 15), which had focal or multifocal granulomatous lesions with few or no AFB. In the multibacillary group (MB), IS900 PCR detected 19 (82.6%), and qPCR detected all 23 (100%) sheep positive for MAP in the intestine and lymph node tissues. In the paucibacillary group (PB), IS900 PCR detected 2 (13.3%), and qPCR detected all 15 (100%) sheep positive for MAP in tissues. When results of both groups were taken together, IS900 PCR detected 21(55.2%), and qPCR detected all 38 (100%) animals positive for MAP genome either in the intestine or lymph node tissues. On Herrold egg yolk medium, tissues of 14 (60.9%) MB and 5 (33.3%) PB sheep were found to be positive for MAP. Out of 27 sheep (PB = 8, MB = 19) tested by an ELISA, 21 (77.7%) were found to be positive for MAP antibody, of which 25% (2/8) and 100% (19/19) sheep were from PB and MB sheep, respectively. Based on the results of the present study, it was concluded that qPCR was a highly sensitive test in comparison to conventional PCR, ELISA and bacterial culture for the diagnosis of paratuberculosis on infected tissues especially from paucibacillary sheep.

  16. Diagnostic accuracy of uriSed automated urine microscopic sediment analyzer and dipstick parameters in predicting urine culture test results.

    Science.gov (United States)

    Huysal, Kağan; Budak, Yasemin U; Karaca, Ayse Ulusoy; Aydos, Murat; Kahvecioğlu, Serdar; Bulut, Mehtap; Polat, Murat

    2013-01-01

    Urinary tract infection (UTI) is one of the most common types of infection. Currently, diagnosis is primarily based on microbiologic culture, which is time- and labor-consuming. The aim of this study was to assess the diagnostic accuracy of urinalysis results from UriSed (77 Electronica, Budapest, Hungary), an automated microscopic image-based sediment analyzer, in predicting positive urine cultures. We examined a total of 384 urine specimens from hospitalized patients and outpatients attending our hospital on the same day for urinalysis, dipstick tests and semi-quantitative urine culture. The urinalysis results were compared with those of conventional semiquantitative urine culture. Of 384 urinary specimens, 68 were positive for bacteriuria by culture, and were thus considered true positives. Comparison of these results with those obtained from the UriSed analyzer indicated that the analyzer had a specificity of 91.1%, a sensitivity of 47.0%, a positive predictive value (PPV) of 53.3% (95% confidence interval (CI) = 40.8-65.3), and a negative predictive value (NPV) of 88.8% (95% CI = 85.0-91.8%). The accuracy was 83.3% when the urine leukocyte parameter was used, 76.8% when bacteriuria analysis of urinary sediment was used, and 85.1% when the bacteriuria and leukocyturia parameters were combined. The presence of nitrite was the best indicator of culture positivity (99.3% specificity) but had a negative likelihood ratio of 0.7, indicating that it was not a reliable clinical test. Although the specificity of the UriSed analyzer was within acceptable limits, the sensitivity value was low. Thus, UriSed urinalysis resuIts do not accurately predict the outcome of culture.

  17. Evaluation of patient safety culture among Malaysian retail pharmacists: results of a self-reported survey.

    Science.gov (United States)

    Sivanandy, Palanisamy; Maharajan, Mari Kannan; Rajiah, Kingston; Wei, Tan Tyng; Loon, Tan Wee; Yee, Lim Chong

    2016-01-01

    Patient safety is a major public health issue, and the knowledge, skills, and experience of health professionals are very much essential for improving patient safety. Patient safety and medication error are very much associated. Pharmacists play a significant role in patient safety. The function of pharmacists in the medication use process is very different from medical and nursing colleagues. Medication dispensing accuracy is a vital element to ensure the safety and quality of medication use. To evaluate the attitude and perception of the pharmacist toward patient safety in retail pharmacies setup in Malaysia. A Pharmacy Survey on Patient Safety Culture questionnaire was used to assess patient safety culture, developed by the Agency for Healthcare Research and Quality, and the convenience sampling method was adopted. The overall positive response rate ranged from 31.20% to 87.43%, and the average positive response rate was found to be 67%. Among all the eleven domains pertaining to patient safety culture, the scores of "staff training and skills" were less. Communication openness, and patient counseling are common, but not practiced regularly in the Malaysian retail pharmacy setup compared with those in USA. The overall perception of patient safety of an acceptable level in the current retail pharmacy setup. The study revealed that staff training, skills, communication in patient counseling, and communication across shifts and about mistakes are less in current retail pharmacy setup. The overall perception of patient safety should be improved by educating the pharmacists about the significance and essential of patient safety.

  18. Culture-based Calibration of the Benthic Foraminiferal Mg/Ca Paleothermometer: Initial Results

    Science.gov (United States)

    Lincoln, S. A.; Filipsson, H. L.; Bernhard, J. M.; McCorkle, D. C.; Shimizu, N.; Birdwhistell, S. P.

    2007-12-01

    The magnesium calcium (Mg/Ca) paleothermometer has become a widely used tool for estimating deep water temperatures. To date, calibrations of the proxy have relied on core-top samples; in such studies, water chemistry and biological factors including food supply often co-vary with temperature, making it difficult to isolate the true Mg/Ca / temperature relationship. A multi-temperature culture experiment was conducted at the Woods Hole Oceanographic Institution from December 2006 through May 2007 in order to study the relationship between Mg/Ca and temperature under controlled conditions. Several species of benthic foraminifera were collected from four locations (the Skagerrak and Gullmar Fjord, Sweden; the Bahamas; and the Charleston Bump, United States; 70 to 800 m water depth), and were grown in microcosms under known, constant physical and chemical conditions at 3.5, 7.0, 14.0, and 21 C. Bulimina species ( B. aculeata and B. marginata) were the most successful, reproducing at 7.0 and 14.0 C and adding chambers at all temperatures. These newly added chambers are the focus of our first Mg/Ca analyses. Because cultured benthic foraminifera are typically lightly calcified, sensitive microanalytical techniques with high spatial resolution are required to measure trace element concentrations in single chambers of cultured specimens. We have explored the use of both secondary ion mass spectrometry and laser ablation inductively coupled mass spectrometry for this application, and present preliminary Mg/Ca data from Bulimina species across the experimental temperature range.

  19. Storytelling Slide Shows to Improve Diabetes and High Blood Pressure Knowledge and Self-Efficacy: Three-Year Results among Community Dwelling Older African Americans

    Science.gov (United States)

    Bertera, Elizabeth M.

    2014-01-01

    This study combined the African American tradition of oral storytelling with the Hispanic medium of "Fotonovelas." A staggered pretest posttest control group design was used to evaluate four Storytelling Slide Shows on health that featured community members. A total of 212 participants were recruited for the intervention and 217 for the…

  20. Storytelling Slide Shows to Improve Diabetes and High Blood Pressure Knowledge and Self-Efficacy: Three-Year Results among Community Dwelling Older African Americans

    Science.gov (United States)

    Bertera, Elizabeth M.

    2014-01-01

    This study combined the African American tradition of oral storytelling with the Hispanic medium of "Fotonovelas." A staggered pretest posttest control group design was used to evaluate four Storytelling Slide Shows on health that featured community members. A total of 212 participants were recruited for the intervention and 217 for the…

  1. A study on factors of dissatisfaction and stress of the blacksmiths resulting from the organizational culture in the surgical instrument industry of India

    Directory of Open Access Journals (Sweden)

    Tirthankar Ghosh

    2011-01-01

    Full Text Available Background: It is important to understand what motivates workers and the extent to which the organization and other contextual variables satisfy them. The aim of the study was to determine factors of dissatisfaction resulting from the organizational culture among the blacksmiths involved in the surgical instrument industry. Materials and Methods: Fifty male surgical blacksmiths each of the skilled and unskilled groups of the forging section were selected. Organizational Role Stress Scale was used to measure the individuals′ role stress and several forms of conflict within an organization. Also, the organizational culture and personal involvement in an organization was measured among the surgical blacksmiths. Results: The mean score for total role stress for Skilled was 71.7 and for unskilled was 77.2. The most frequent type of organizational culture was reported to be hierarchy, both by skilled and unskilled surgical blacksmiths, followed by market and clan culture. Conclusion: This study shows that the skilled surgical blacksmiths have lower level of stress and conflicts in comparison with unskilled surgical blacksmiths. Both skilled and unskilled surgical blacksmiths estimated their level of personal involvement as low and indicated insufficient involvement in work teams. The satisfaction of the employees with their status and role in the organizational culture was also poor for both skilled and unskilled surgical blacksmiths.

  2. Embryos cultured in a time-lapse system result in superior treatment outcomes: a strict matched pair analysis.

    Science.gov (United States)

    Barrie, Amy; Homburg, Roy; McDowell, Garry; Brown, Jeremy; Kingsland, Charles; Troup, Stephen

    2017-09-01

    A retrospective strict matched-pair analysis of 728 treatment cycles between January 2011 and September 2014 was performed. A total of 364 treatment cycles, where all embryos were cultured and examined in EmbryoScope(®), were matched to treatment cycles where all the embryos were cultured in a standard incubator with conventional morphological examination. Matching was performed for patient age, number of oocytes collected, treatment type and date of oocyte collection (± six months). The clinical (CPR), implantation (IR), live birth (LBR) and miscarriage rates (MR) were calculated and considered significant when p result was not statistically significant (18.9% versus 24.4%, p = 0.19). There is a paucity of well-designed studies to confirm that embryos cultured and examined in TLS can result in superior treatment outcomes, and this strict-matched pair analysis with a large cohort of treatment cycles indicates the advantage of using TLS.

  3. An evaluation of culture results during treatment for tuberculosis as surrogate endpoints for treatment failure and relapse.

    Directory of Open Access Journals (Sweden)

    Patrick P J Phillips

    Full Text Available It is widely acknowledged that new regimens are urgently needed for the treatment of tuberculosis. The primary endpoint in the Phase III trials is a composite outcome of failure at the end of treatment or relapse after stopping treatment. Such trials are usually both long and expensive. Valid surrogate endpoints measured during or at the end of treatment could dramatically reduce both the time and cost of assessing the effectiveness of new regimens. The objective of this study was to evaluate sputum culture results on solid media during treatment as surrogate endpoints for poor outcome. Data were obtained from twelve randomised controlled trials conducted by the British Medical Research Council in the 1970s and 80s in East Africa and East Asia, consisting of 6974 participants and 49 different treatment regimens. The month two culture result was shown to be a poor surrogate in East Africa but a good surrogate in Hong Kong. In contrast, the month three culture was a good surrogate in trials conducted in East Africa but not in Hong Kong. As well as differences in location, ethnicity and probable strain of Mycobacteria tuberculosis, Hong Kong trials more often evaluated regimens with rifampicin throughout and intermittent regimens, and patients in East African trials more often presented with extensive cavitation and were slower to convert to culture negative during treatment. An endpoint that is a summary measure of the longitudinal profile of culture results over time or that is able to detect the presence of M. tuberculosis later in treatment is more likely to be a better endpoint for a phase II trial than a culture result at a single time point and may prove to be an acceptable surrogate. More data are needed before any endpoint can be used as a surrogate in a confirmatory phase III trial.

  4. Randomised control trial showed that delayed cord clamping and milking resulted in no significant differences in iron stores and physical growth parameters at one year of age.

    Science.gov (United States)

    Agarwal, Shivam; Jaiswal, Vijay; Singh, Dharamveer; Jaiswal, Prateek; Garg, Amit; Upadhyay, Amit

    2016-11-01

    Placental redistribution has been shown to improve haematological outcomes in the immediate neonatal period and early infancy. This study compared the effects of delayed cord clamping (DCC) and umbilical cord milking (UCM) on haematological and growth parameters at 12 months of age. This was a follow-up study of a randomised control trial, conducted in a tertiary care paediatric centre from August 2013 to August 2014. We studied 200 apparently healthy Indian infants randomised at birth to receive DCC for 60-90 seconds or UCM. The outcome measures were iron status and physical growth parameters at 12 months. Of the 200 babies, 161 completed the follow-up and baseline characteristics were comparable in both groups. The mean haemoglobin in the DCC group (102.2 (17.2) g/L and serum ferritin 16.44 (2.77) μg/L) showed no significant differences to the UCM group (98.6 (17.1) g/L and 18.2 (2.8) μg/L) at one year. In addition, there were no significant differences in weight, height and mid-upper arm circumference in the two groups. Term-born Indian infants who had DCC at 60-90 seconds or UCM showed no significant differences in ferritin and haemoglobin levels and growth parameters at 12 months of age. ©2016 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.

  5. The Cultivation Theory: AResearch Towards to the Ideological Cultural Results of Living with Television

    OpenAIRE

    Omer Ozer

    2005-01-01

    In this study, we try find out if there is a role of television on the people’s per- ception of the world and their characterization of the social reality. In other words, we investigate the television’s cultivation role. To investigate the television’s cultivation role, we use the components of Cultural Indicators Project of George Gerbner such as Cultivation Analysis and Message System. Analysis To investigate the television’s cultiva- tion role, first of all, we analyze the prime t...

  6. Showing results? An analysis of the perceptions of internal and external stakeholders of the public performance communication by the Belgian and Dutch Railways

    NARCIS (Netherlands)

    Gelders, Dave; Galetzka, Mirjam; Verckens, Jan Pieter; Seydel, E.R.

    2008-01-01

    Information Quarterly, 15: 153–156). In Belgium and the Netherlands, one Performance measurement and communicating about it with the broader public is not self-evident if one looks at public services organizations (Hernon, 1998 P. Hernon, The government performance and results act. Government

  7. Showing results? An analysis of the perceptions of internal and external stakeholders of the public performance communication by the Belgian and Dutch Railways

    NARCIS (Netherlands)

    Gelders, Dave; Galetzka, Mirjam; Verckens, Jan Pieter; Seydel, Erwin

    2008-01-01

    Information Quarterly, 15: 153–156). In Belgium and the Netherlands, one Performance measurement and communicating about it with the broader public is not self-evident if one looks at public services organizations (Hernon, 1998 P. Hernon, The government performance and results act. Government Inform

  8. The relevance of external quality assessment for molecular testing for ALK positive non-small cell lung cancer: results from two pilot rounds show room for optimization.

    Directory of Open Access Journals (Sweden)

    Lien Tembuyser

    Full Text Available Molecular profiling should be performed on all advanced non-small cell lung cancer with non-squamous histology to allow treatment selection. Currently, this should include EGFR mutation testing and testing for ALK rearrangements. ROS1 is another emerging target. ALK rearrangement status is a critical biomarker to predict response to tyrosine kinase inhibitors such as crizotinib. To promote high quality testing in non-small cell lung cancer, the European Society of Pathology has introduced an external quality assessment scheme. This article summarizes the results of the first two pilot rounds organized in 2012-2013.Tissue microarray slides consisting of cell-lines and resection specimens were distributed with the request for routine ALK testing using IHC or FISH. Participation in ALK FISH testing included the interpretation of four digital FISH images.Data from 173 different laboratories was obtained. Results demonstrate decreased error rates in the second round for both ALK FISH and ALK IHC, although the error rates were still high and the need for external quality assessment in laboratories performing ALK testing is evident. Error rates obtained by FISH were lower than by IHC. The lowest error rates were observed for the interpretation of digital FISH images.There was a large variety in FISH enumeration practices. Based on the results from this study, recommendations for the methodology, analysis, interpretation and result reporting were issued. External quality assessment is a crucial element to improve the quality of molecular testing.

  9. Non-Neuronal Cells Are Required to Mediate the Effects of Neuroinflammation: Results from a Neuron-Enriched Culture System.

    Directory of Open Access Journals (Sweden)

    Chin Wai Hui

    Full Text Available Chronic inflammation is associated with activated microglia and reactive astrocytes and plays an important role in the pathogenesis of neurodegenerative diseases such as Alzheimer's. Both in vivo and in vitro studies have demonstrated that inflammatory cytokine responses to immune challenges contribute to neuronal death during neurodegeneration. In order to investigate the role of glial cells in this phenomenon, we developed a modified method to remove the non-neuronal cells in primary cultures of E16.5 mouse cortex. We modified previously reported methods as we found that a brief treatment with the thymidine analog, 5-fluorodeoxyuridine (FdU, is sufficient to substantially deplete dividing non-neuronal cells in primary cultures. Cell cycle and glial markers confirm the loss of ~99% of all microglia, astrocytes and oligodendrocyte precursor cells (OPCs. More importantly, under this milder treatment, the neurons suffered neither cell loss nor any morphological defects up to 2.5 weeks later; both pre- and post-synaptic markers were retained. Further, neurons in FdU-treated cultures remained responsive to excitotoxicity induced by glutamate application. The immunobiology of the FdU culture, however, was significantly changed. Compared with mixed culture, the protein levels of NFκB p65 and the gene expression of several cytokine receptors were altered. Individual cytokines or conditioned medium from β-amyloid-stimulated THP-1 cells that were, potent neurotoxins in normal, mixed cultures, were virtually inactive in the absence of glial cells. The results highlight the importance of our glial-depleted culture system and identifies and offer unexpected insights into the complexity of -brain neuroinflammation.

  10. Organizational culture and the implementation of person centered care: results from a change process in Swedish hospital care.

    Science.gov (United States)

    Alharbi, Tariq Saleem J; Ekman, Inger; Olsson, Lars-Eric; Dudas, Kerstin; Carlström, Eric

    2012-12-01

    Sweden has one of the oldest, most coherent and stable healthcare systems in the world. The culture has been described as conservative, mechanistic and increasingly standardized. In order to provide a care adjusted to the patient, person centered care (PCC) has been developed and implemented into some parts of the health care industry. The model has proven to decrease patient uncertainty. However, the impact of PCC has been limited in some clinics and hospital wards. An assumption is that organizational culture has an impact on desired outcomes of PCC, such as patient uncertainty. Therefore, in this study we identify the impact of organizational culture on patient uncertainty in five hospital wards during the implementation of PCC. Data from 220 hospitalized patients who completed the uncertainty cardiovascular population scale (UCPS) and 117 nurses who completed the organizational values questionnaire (OVQ) were investigated with regression analysis. The results seemed to indicate that in hospitals where the culture promotes stability, control and goal setting, patient uncertainty is reduced. In contrast to previous studies suggesting that a culture of flexibility, cohesion and trust is positive, a culture of stability can better sustain a desired outcome of reform or implementation of new care models such as person centered care. It is essential for health managers to be aware of what characterizes their organizational culture before attempting to implement any sort of new healthcare model. The organizational values questionnaire has the potential to be used as a tool to aid health managers in reaching that understanding. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  11. On Cultural Empathy of Chinese Movies and TV Shows for Intercultural Communication%跨文化交际视角下影视剧的文化移情

    Institute of Scientific and Technical Information of China (English)

    余爱菊

    2014-01-01

    由于受不同的思维方式、价值观念和审美认知等因素影响,影视产品在对外传播过程中会遭遇交流障碍。为克服交流屏障,提高影视艺术品的传播效果,我国影视剧应从跨文化交际视角观照题材选择和艺术风格表现,适当运用文化移情策略,以得到海外观众的理解和认同。影视剧的文化移情包括题材移情,思想主题移情和审美移情三个方面。%Movies and TV shows will meet with barriers in intercultural communication due to differences in way of thinking ,value and aesthetic cognition .In order to promote the circulation of Chinese cultural products ,we can exert cultural empathy for the target audience to appreciate .Cultural empathy includes selecting the subject matter which can reflect the essence of Chinese culture and the theme that merging Chinese with western cultures , and adapting to the expectations of their aesthetic cognition .

  12. Cyberbullying as a negative result of cyber-culture of Slovak children and adolescents: selected research findings

    Directory of Open Access Journals (Sweden)

    Hollá Katarína

    2016-05-01

    Full Text Available Cyber-culture points out the life in cyberspace and goes beyond national cultures. It is particularly attractive for the young people who use Information and Communications Technologies (ICT to express their attitudes, values, beliefs and thinking. Those do not need to be necessarily in accordance with the standards of an individual society. Cyberculture becomes dangerous. Great risk lies in cyberbullying that represents negative impact of cyber-culture on human behavior. The aim of the study is to detect cyberbullying as a negative impact of cyber-culture among of Slovak children and adolescents. The research was carried out on a sample of 1619 11-18-year old respondents (average age was 14.51. Results of cyberbullying research carried out using Latent Class Analysis (LCA have proved the appropriateness of 3-latent-class module. Relative entropy of the module reached 0.915. It was demonstrated that 52.9% of respondents belonged to the group of uninvolved, 42.7% were victims and 4.4% were victims-aggressors. Being a negative consequence of cyber-culture, cyberbullying is a challenge that educators - including other assisting professions - face when educating children and adolescents to orientate in cyberspace, behave responsibly, express themselves in a way that would not interfere others’ integrity and identity (personal and virtual. The study was written under VEGA MŠVVaŠ SR a SAV č. 1/0244/15: “Detekcia a riešenie kyberšikany”.

  13. Mathematical modelling in Matlab of the experimental results shows the electrochemical potential difference - temperature of the WC coatings immersed in a NaCl solution

    Science.gov (United States)

    Benea, M. L.; Benea, O. D.

    2016-02-01

    The method used for purchasing the corrosion behaviour the WC coatings deposited by plasma spraying, on a martensitic stainless steel substrate consists in measuring the electrochemical potential of the coating, respectively that of the substrate, immersed in a NaCl solution as corrosive agent. The mathematical processing of the obtained experimental results in Matlab allowed us to make some correlations between the electrochemical potential of the coating and the solution temperature is very well described by some curves having equations obtained by interpolation order 4.

  14. The Use of Online Pre-Lab Assessments Compared with Written Pre-Lab Assignments Requiring Experimental Result Prediction Shows No Difference in Student Performance

    Directory of Open Access Journals (Sweden)

    Erica L. Suchman

    2015-08-01

    Full Text Available Exam performance was compared for students who hand wrote questions designed to prepare them for daily lab activities in a senior level virology laboratory course versus those who answered questions created to mirror the written questions on-line.  No significant difference was noted in exam scores on any of the three midterms, written final exam, nor the practical exam.  Neither was there a significant difference in the quality of the laboratory reports turned in as evidenced by similar average scores over four years.  These results indicate that using online pre-labs to prepare students for the laboratory sessions leads to equivalent learning as answering handwritten pre-lab assignments.  Online pre-labs significantly reduced the amount of grading without reducing student learning, allowing a reduction in the number of teaching assistants required per section.

  15. Cells exposed to a huntingtin fragment containing an expanded polyglutamine tract show no sign of ion channel formation: results arguing against the ion channel hypothesis

    DEFF Research Database (Denmark)

    Nørremølle, Anne; Grunnet, Morten; Hasholt, Lis

    2003-01-01

    tract to form ion channels in two cell types. Whole cell current from Xenopus oocytes was recorded using two-electrode voltage-clamp technique, and whole cell current from CHO-K1 cells was recorded by patch-clamp technique. The fragment with an expanded polyglutamine sequence induced no change......Ion channels formed by expanded polyglutamine tracts have been proposed to play an important role in the pathological processes leading to neurodegeneration in Huntington's disease and other CAG repeat diseases. We tested the capacity of a huntingtin fragment containing an expanded polyglutamine...... in the currents recorded in any of the two expression systems, indicating no changes in ion channel activity. The results therefore argue against the proposed hypothesis of expanded polyglutamines forming ion channels....

  16. Value of Fused 18F-Choline-PET/MRI to Evaluate Prostate Cancer Relapse in Patients Showing Biochemical Recurrence after EBRT: Preliminary Results

    Directory of Open Access Journals (Sweden)

    Arnoldo Piccardo

    2014-01-01

    Full Text Available Purpose. We compared the accuracy of 18F-Choline-PET/MRI with that of multiparametric MRI (mMRI, 18F-Choline-PET/CT, 18F-Fluoride-PET/CT, and contrast-enhanced CT (CeCT in detecting relapse in patients with suspected relapse of prostate cancer (PC after external beam radiotherapy (EBRT. We assessed the association between standard uptake value (SUV and apparent diffusion coefficient (ADC. Methods. We evaluated 21 patients with biochemical relapse after EBRT. Patients underwent 18F-Choline-PET/contrast-enhanced (CeCT, 18F-Fluoride-PET/CT, and mMRI. Imaging coregistration of PET and mMRI was performed. Results. 18F-Choline-PET/MRI was positive in 18/21 patients, with a detection rate (DR of 86%. DRs of 18F-Choline-PET/CT, CeCT, and mMRI were 76%, 43%, and 81%, respectively. In terms of DR the only significant difference was between 18F-Choline-PET/MRI and CeCT. On lesion-based analysis, the accuracy of 18F-Choline-PET/MRI, 18F-Choline-PET/CT, CeCT, and mMRI was 99%, 95%, 70%, and 85%, respectively. Accuracy, sensitivity, and NPV of 18F-Choline-PET/MRI were significantly higher than those of both mMRI and CeCT. On whole-body assessment of bone metastases, the sensitivity of 18F-Choline-PET/CT and 18F-Fluoride-PET/CT was significantly higher than that of CeCT. Regarding local and lymph node relapse, we found a significant inverse correlation between ADC and SUV-max. Conclusion. 18F-Choline-PET/MRI is a promising technique in detecting PC relapse.

  17. Analysis on difference between manual and apparatus blood culture results%手工和仪器两种血培养结果差异性分析

    Institute of Scientific and Technical Information of China (English)

    张国荣; 彭松庆; 张秋

    2012-01-01

    Objective To retrospectively analyze the positive rates in the manual method of biphasic blood culture bottle and BACTEC9120 automated blood culture system. Methods The blood samples were inoculated at the same time onto biphasic blood culture bottle and BACTEC9120 automated blood culture bottle with instrument supporting. The positive results were transplanted onto blood culture medium, while the negative ones were transplanted onto chocolate culture medium. Resultsln the 370 samples of blood culture, there were 25 positive samples in biphasic blood culture bottle, and the positive rate was 6. 76% (25/370) . 59 samples of resin aerobic ( children) bottles were alarmed positively by BACTEC9120, and the positive rate was 15.9% (59/370). 54 samples showed positive results after being transplanted onto blood culture mediums and chocolate culture mediums, the positive rate was 14.6% (54/370). 5 samples were false positive, and the false positive rate was 1.4% (5/370). A total of 29 patients resin aerobic (children) bottle was positive, while the biphasic blood culture bottle was negative. There were highly significant difference in two kinds of culture methods ( P < 0. 001 ). Conclusion BACTEC9120 automated blood culture system can improve the positive rate, shorten the positive reports time better than the traditional biphasic blood culture medium.%目的 对手工法双相血培养瓶和BACTEC9120全自动血培养仪的阳性率作回顾性分析.方法 将血液标本同时接种双相血培养基和BACTEC9120全自动血培养仪配套血瓶中,将阳性结果移种血平板,如为阴性再移种巧克力平板.结果 370例血培养,双相血培养瓶阳性25例,阳性率为6.76% (25/370),树脂需氧(儿童)瓶BACTEC9120报警显示阳性59例,阳性率为15.9% (59/370),阳性标本移种到血平板及巧克力平板阳性54例,阳性率为14.6% (54/370),假阳性5例,假阳性率为1.4% (5/370),共有29例树脂需氧(儿童)瓶阳性,而

  18. Cultural competency in peer-run programs: results of a web survey and implications for future practice.

    Science.gov (United States)

    Jonikas, Jessica A; Kiosk, Stephen; Grey, Dennis D; Hamilton, Marie M; McNulty, James; Cook, Judith A

    2010-01-01

    The study explored perceptions of adults with psychiatric disabilities regarding cultural competency of peer-run mental health support groups and programs. Web survey respondents were recruited via mental health list-servs, web sites, newsletters, emails, and word of mouth. A total of 527 peers were surveyed about cultural competency barriers facing peer-run programs; common reasons for not using peer services; and strategies to engage diverse communities. Both multicultural and Caucasian respondents agreed that lack of funding and staff education about diversity were barriers to cultural competency in peer programs. Multicultural respondents were more likely than whites to feel that both the recognition of the need for and interest in attending cultural competency training is lacking in peer programs, as well as information about the diverse composition of peer program memberships. Among those who had never participated in peer support, people of color were more likely than whites to endorse feeling they would not belong and believing their languages would not be spoken in peer programs. Whites, on the other hand, were more likely to cite a preference for professional over peer support, while nearly half of both groups indicated that the main reason for non-attendance is a lack of knowledge about peer programs. Qualitative results highlighted successful outreach and engagement strategies. Study findings informed development of a cultural competency tool that was pilot-tested among peer-run programs. Given the importance of peer support in recovery, these findings suggest the need for additional research on cultural competency in peer programs.

  19. Clinical features, cytology and bacterial culture results in dogs with and without cheilitis and comparison of three sampling techniques.

    Science.gov (United States)

    Doelle, Maren; Loeffler, Anette; Wolf, Katharina; Kostka, Veit; Linek, Monika

    2016-06-01

    Cheilitis is a common presentation in dogs associated with a variety of skin diseases and often complicated by microbial infections. To describe and compare clinical and cytological features and bacterial culture results from the lower lips of dogs with cheilitis (as compared to healthy controls), and to evaluate three cytology sampling techniques for their abilities to differentiate between the groups. Fifty six dogs with cheilitis and 54 controls. Anatomy and clinical signs of the lower lip were recorded. Cytology samples taken by tape strip, direct impression and swabs rolled over skin were scored semiquantitatively for microorganisms, inflammatory cells and keratinocytes. Cytology scores were correlated with semiquantitative bacterial culture scores. Pure breeds, frequency of lip folds and all cytology scores except keratinocytes were higher in dogs with cheilitis than in controls, but a substantial overlap was seen in all microorganisms between the groups. Hypersensitivity disorders were diagnosed in 40 of 56 dogs with cheilitis. The tape strip technique yielded the greatest differences between groups. Bacterial growth was reported in 100% of dogs with cheilitis and in 93% of the controls. Pathogens such as Staphylococcus pseudintermedius, Escherichia coli and Pseudomonas spp were found more frequently in dogs with cheilitis. Cytology and bacterial culture were poorly correlated. Cheilitis was associated with primary hypersensitivity disorders and the presence of a lip fold was a predisposing factor. Results of aerobic culture were similar to prior studies on pyoderma of other body sites, except for higher rates of Pseudomonas spp. isolation. © 2016 ESVD and ACVD.

  20. Fast Filtration of Bacterial or Mammalian Suspension Cell Cultures for Optimal Metabolomics Results.

    Science.gov (United States)

    Bordag, Natalie; Janakiraman, Vijay; Nachtigall, Jonny; González Maldonado, Sandra; Bethan, Bianca; Laine, Jean-Philippe; Fux, Elie

    2016-01-01

    The metabolome offers real time detection of the adaptive, multi-parametric response of the organisms to environmental changes, pathophysiological stimuli or genetic modifications and thus rationalizes the optimization of cell cultures in bioprocessing. In bioprocessing the measurement of physiological intracellular metabolite levels is imperative for successful applications. However, a sampling method applicable to all cell types with little to no validation effort which simultaneously offers high recovery rates, high metabolite coverage and sufficient removal of extracellular contaminations is still missing. Here, quenching, centrifugation and fast filtration were compared and fast filtration in combination with a stabilizing washing solution was identified as the most promising sampling method. Different influencing factors such as filter type, vacuum pressure, washing solutions were comprehensively tested. The improved fast filtration method (MxP® FastQuench) followed by routine lipid/polar extraction delivers a broad metabolite coverage and recovery reflecting well physiological intracellular metabolite levels for different cell types, such as bacteria (Escherichia coli) as well as mammalian cells chinese hamster ovary (CHO) and mouse myeloma cells (NS0).The proposed MxP® FastQuench allows sampling, i.e. separation of cells from medium with washing and quenching, in less than 30 seconds and is robustly designed to be applicable to all cell types. The washing solution contains the carbon source respectively the 13C-labeled carbon source to avoid nutritional stress during sampling. This method is also compatible with automation which would further reduce sampling times and the variability of metabolite profiling data.

  1. Prenatal diagnosis and selective abortion: a result of the cultural turn?

    Science.gov (United States)

    Bromage, D I

    2006-06-01

    There is a growing trend in obstetric medicine of prenatal diagnosis and the selective abortion of foetuses that are likely to be born with a disability. Reasons commonly given to explain this trend include the financial implications of screening and testing policies, the disruption to families caused by the birth of a child with a disability, and the potential quality of life of the unborn child. This paper reflects upon another possible reason for this. It is argued that it is, in part, a consequence of our attitudes towards disability and a pursuit of aesthetic perfection. These attitudes arise from a social context that may be explained by considering the effect on the disabled community of the transition from modernity to postmodernity. This shift is demonstrated by inspecting some of the synonymous developments in art history. It is suggested that this "cultural turn" may have both helped and hindered people with disabilities, but the hypothesis requires further testing. This could best be achieved with a qualitative study of what motivates parental decision making in the obstetric unit.

  2. Research culture and capacity in community health services: results of a structured survey of staff.

    Science.gov (United States)

    Friesen, Emma L; Comino, Elizabeth J

    2016-08-17

    Developing research capacity is recognised as an important endeavour. However, little is known about the current research culture, capacity and supports for staff working in community-based health settings. A structured survey of Division of Community Health staff was conducted using the research capacity tool. The survey was disseminated by email and in paper format. Quantitative data were analysed using descriptive statistics. Qualitative data were analysed thematically. In total, 109 usable responses were received, giving a response rate of 26%. Respondents were predominately nurses (n=71, 65.7%), with ~50% reporting post-graduate vocational qualifications. The highest levels of skills or organisational success were in using evidence to plan, promote and guide clinical practice. Most participants were unsure of organisational and team level skills and success at generating research. Few reported recent experience in research-generating activities. Barriers to undertaking research included lack of skills, time and access to external support and funding. Lack of skills and success in accessing external funding and resources to protect research time or to 'buy-in' technical expertise appeared to exacerbate these barriers. Community health staff have limited capacity to generate research with current levels of skill, funding and time. Strategies to increase research capacity should be informed by knowledge of clinicians' research experience and interests, and target development of skills to generate research. Resources and funding are needed at the organisational and team levels to overcome the significant barriers to research generation reported.

  3. SisterTalk: final results of a culturally tailored cable television delivered weight control program for Black women.

    Science.gov (United States)

    Risica, Patricia Markham; Gans, Kim M; Kumanyika, Shiriki; Kirtania, Usree; Lasater, Thomas M

    2013-12-27

    Obesity among Black women continues to exceed that of other women. Most weight loss programs created without reference to specific cultural contexts are less effective for Black than White women. Weight control approaches accessible to Black women and adapted to relevant cultural contexts are important for addressing this problem. This paper reports the final results of SisterTalk, the randomized controlled trial of a cable TV weight control program oriented toward Black women. A five group design included a comparison group and a 2 × 2 factorial comparison of a) interactive vs. passive programming and b) telephone social support vs no telephone support, with 12 weekly initial cable TV programs followed by 4 monthly booster videos. At baseline, 3, 8, and 12 months post randomization, telephone and in person surveys were administered on diet, physical activity, and physical measurements of height and weight were taken to calculate body mass index (BMI). Analysis of variance (ANOVA) was used to examine differences over time, and between treatment and comparison groups. Dose variables reflecting use of the TV/video and written materials were also assessed. At 3 months, BMI, weight, and dietary fat were significantly lower and physical activity significantly higher among women exposed to the Cable TV intervention compared to the wait-list comparison group. Significant dietary fat differences were still observed at 8 and 12 month evaluations, but not BMI or physical activity differences. Main effects were not observed for interactive programming or enhanced social support at any time point. Within the intervention group, higher watching of the TV series and higher reading of educational materials were both (separately) associated with significantly lower dietary fat. Cable TV was an effective delivery channel to assist Black women with weight control, increasing physical activity and decreasing dietary fat during an initial intervention period, but only dietary

  4. 论交友类真人秀节目在我国电视审美文化地位的凸显%On Position Highlight of Dating Reality Show Program in Our TV Aesthetic Culture

    Institute of Scientific and Technical Information of China (English)

    解芳

    2012-01-01

    Dating reality show program is spread into our country from Euro-American countries. At the background of global culture, it is a new program form which takes visual turn as the premise. At the same time, it is different from the western dating reality show programs. It contains our traditional cultural thought, shows our program character with Chinese characteristics, and is a typical representative of the present TV aesthetic culture.%交友类真人秀节目由欧美国家传入我国,它是在文化全球化的大背景下,以视觉转向为前提的一种新的节目形式。同时,它又区别于西方的交友类真人秀节目,蕴含着我国的传统文化思想,昭示出具有中国特色的节目品格,是当代电视审美文化中的典型代袁。

  5. Fast Filtration of Bacterial or Mammalian Suspension Cell Cultures for Optimal Metabolomics Results.

    Directory of Open Access Journals (Sweden)

    Natalie Bordag

    Full Text Available The metabolome offers real time detection of the adaptive, multi-parametric response of the organisms to environmental changes, pathophysiological stimuli or genetic modifications and thus rationalizes the optimization of cell cultures in bioprocessing. In bioprocessing the measurement of physiological intracellular metabolite levels is imperative for successful applications. However, a sampling method applicable to all cell types with little to no validation effort which simultaneously offers high recovery rates, high metabolite coverage and sufficient removal of extracellular contaminations is still missing. Here, quenching, centrifugation and fast filtration were compared and fast filtration in combination with a stabilizing washing solution was identified as the most promising sampling method. Different influencing factors such as filter type, vacuum pressure, washing solutions were comprehensively tested. The improved fast filtration method (MxP® FastQuench followed by routine lipid/polar extraction delivers a broad metabolite coverage and recovery reflecting well physiological intracellular metabolite levels for different cell types, such as bacteria (Escherichia coli as well as mammalian cells chinese hamster ovary (CHO and mouse myeloma cells (NS0.The proposed MxP® FastQuench allows sampling, i.e. separation of cells from medium with washing and quenching, in less than 30 seconds and is robustly designed to be applicable to all cell types. The washing solution contains the carbon source respectively the 13C-labeled carbon source to avoid nutritional stress during sampling. This method is also compatible with automation which would further reduce sampling times and the variability of metabolite profiling data.

  6. The culture of cancer cell lines as tumorspheres does not systematically result in cancer stem cell enrichment.

    Science.gov (United States)

    Calvet, Christophe Y; André, Franck M; Mir, Lluis M

    2014-01-01

    Cancer stem cells (CSC) have raised great excitement during the last decade and are promising targets for an efficient treatment of tumors without relapses and metastases. Among the various methods that enable to enrich cancer cell lines in CSC, tumorspheres culture has been predominantly used. In this report, we attempted to generate tumorspheres from several murine and human cancer cell lines: B16-F10, HT-29, MCF-7 and MDA-MB-231 cells. Tumorspheres were obtained with variable efficiencies from all cell lines except from MDA-MB-231 cells. Then, we studied several CSC characteristics in both tumorspheres and adherent cultures of the B16-F10, HT-29 and MCF-7 cells. Unexpectedly, tumorspheres-forming cells were less clonogenic and, in the case of B16-F10, less proliferative than attached cells. In addition, we did not observe any enrichment in the population expressing CSC surface markers in tumorspheres from B16-F10 (CD133, CD44 and CD24 markers) or MCF-7 (CD44 and CD24 markers) cells. On the contrary, tumorspheres culture of HT-29 cells appeared to enrich in cells expressing colon CSC markers, i.e. CD133 and CD44 proteins. For the B16-F10 cell line, when 1 000 cells were injected in syngenic C57BL/6 mice, tumorspheres-forming cells displayed a significantly lower tumorigenic potential than adherent cells. Finally, tumorspheres culture of B16-F10 cells induced a down-regulation of vimentin which could explain, at least partially, the lower tumorigenicity of tumorspheres-forming cells. All these results, along with the literature, indicate that tumorspheres culture of cancer cell lines can induce an enrichment in CSC but in a cell line-dependent manner. In conclusion, extensive characterization of CSC properties in tumorspheres derived from any cancer cell line or cancer tissue must be performed in order to ensure that the generated tumorspheres are actually enriched in CSC.

  7. Evaluation of routinely reported surgical site infections against microbiological culture results: a tool to identify patient groups where diagnosis and treatment may be improved

    Directory of Open Access Journals (Sweden)

    Kievit Job

    2009-11-01

    Full Text Available Abstract Background Surgeons may improve their decision making by assessing the extent to which their initial clinical diagnosis of a surgical site infection (SSI was supported by culture results. Aim of the present study was to evaluate routinely reported SSI by surgeons against microbiological culture results, to identify patient groups with lower agreement where decision making may be improved. Methods 701 admissions with SSI were reported by surgeons in a university medical centre in the period 1997-2005, which were retrospectively checked for microbiological culture results. Reporting a SSI was conditional on treatment being given (e.g. antibiotics and was classified by severity. To identify specific patient groups, patients were classified according to the surgery group of the first operation during admission (e.g. trauma. Results Of all reported SSI, 523 (74.6% had a positive culture result, 102 (14.6% a negative culture result and 76 (10.8% were classified as unknown culture result (due to no culture taken. Given a known culture result, reported SSI with positive culture results less often concerned trauma patients (16% versus 26%, X2 = 4.99 p = 0.03 and less severe SSI (49% versus 85%, X2 = 10.11 p Conclusion Routine reporting of SSI was mostly supported by culture results. However, this support was less often found in trauma patients and less severe SSI, thereby giving surgeons feedback that diagnosis and treatment may be improved in these cases.

  8. Increased bacterial growth efficiency with environmental variability: results from DOC degradation by bacteria in pure culture experiments

    Directory of Open Access Journals (Sweden)

    M. Eichinger

    2010-06-01

    Full Text Available This paper assesses how considering variation in DOC availability and cell maintenance in bacterial models affects Bacterial Growth Efficiency (BGE estimations. For this purpose, we conducted two biodegradation experiments simultaneously. In experiment one, a given amount of substrate was added to the culture at the start of the experiment whilst in experiment two, the same amount of substrate was added, but using periodic pulses over the time course of the experiment. Three bacterial models, with different levels of complexity, (the Monod, Marr-Pirt and the dynamic energy budget – DEB – models, were used and calibrated using the above experiments. BGE has been estimated using the experimental values obtained from discrete samples and from model generated data. Cell maintenance was derived experimentally, from respiration rate measurements. The results showed that the Monod model did not reproduce the experimental data accurately, whereas the Marr-Pirt and DEB models demonstrated a good level of reproducibility, probably because cell maintenance was built into their formula. Whatever estimation method was used, the BGE value was always higher in experiment two (the periodically pulsed substrate as compared to the initially one-pulsed-substrate experiment. Moreover, BGE values estimated without considering cell maintenance (Monod model and empirical formula were always smaller than BGE values obtained from models taking cell maintenance into account. Since BGE is commonly estimated using constant experimental systems and ignore maintenance, we conclude that these typical methods underestimate BGE values. On a larger scale, and for biogeochemical cycles, this would lead to the conclusion that, for a given DOC supply rate and a given DOC consumption rate, these BGE estimation methods overestimate the role of bacterioplankton as CO2 producers.

  9. Increased bacterial growth efficiency with environmental variability: results from DOC degradation by bacteria in pure culture experiments

    Directory of Open Access Journals (Sweden)

    M. Eichinger

    2010-02-01

    Full Text Available This paper assesses how considering variation in DOC availability and cell maintenance in bacterial models affects Bacterial Growth Efficiency (BGE estimations. For this purpose, we conducted two biodegradation experiments simultaneously. In experiment one, a given amount of substrate was added to the culture at the start of the experiment whilst in experiment two, the same amount of substrate was added, but using periodic pulses over the time course of the experiment. Three bacterial models, with different levels of complexity, (the Monod, Marr-Pirt and the dynamic energy budget (DEB model, were used, and calibrated using the above experiments. BGE has been estimated using the experimental values obtained from discrete samples and from model generated data. Cell maintenance was derived experimentally, from respiration rate measurements. The results showed that the Monod model did not reproduce the experimental data accurately, whereas the Marr-Pirt and DEB models demonstrated a good level of reproducibility, probably because cell maintenance was built into their formula. Whatever estimation method was used, the BGE value was always higher in experiment two (the periodically pulsed substrate as compared to the initially one-pulsed-substrate experiment. Moreover, BGE values estimated without considering cell maintenance (Monod model and empirical formula were always smaller than BGE values obtained from models taking cell maintenance into account. Since BGE is commonly estimated using constant experimental systems and ignore maintenance, we conclude that these typical methods underestimate BGE values. On a larger scale, and for biogeochemical cycles, this would lead to the conclusion that, for a given DOC supply rate and a given DOC consumption rate, these BGE estimation methods overestimate the role of bacterioplankton as CO2 producers.

  10. Randomised Response Technique-An Innovative Method To Measure Culturally Sensitive Variables : Results From A Pilot Study

    Directory of Open Access Journals (Sweden)

    Soudarssanane M. B

    2003-01-01

    Full Text Available Research questions: What is the advantage of Randomized Response Technique (RRT over the conventional Direct Interview (DI and Anonymous Questionnaire (AQ in the assessment of culturally sensitive variables? Objectives: To compare the efficacy of the three methods, namely RRT, DI and AQ in the measurement of prevalence of Pre/Extra marital sex. Study design: Cross sectional study, using the three methods. Setting: A pilot study in a given community in Pondicherry. Statistical analysis: Probability equations. Results: The prevalence of pre/extra marital sex in the study population by the DI, AQ and RRT methods were 0%, 6% and 10% respectively in this pilot study. Conclusion: RRT improves validity of measurement of culturally sensitive variables both by ensuring a high participation in the study and by enabling a true response by assuring full confidentiality of information.

  11. Laser induced breakdown spectroscopy for elemental analysis in environmental, cultural heritage and space applications: a review of methods and results.

    Science.gov (United States)

    Gaudiuso, Rosalba; Dell'Aglio, Marcella; De Pascale, Olga; Senesi, Giorgio S; De Giacomo, Alessandro

    2010-01-01

    Analytical applications of Laser Induced Breakdown Spectroscopy (LIBS), namely optical emission spectroscopy of laser-induced plasmas, have been constantly growing thanks to its intrinsic conceptual simplicity and versatility. Qualitative and quantitative analysis can be performed by LIBS both by drawing calibration lines and by using calibration-free methods and some of its features, so as fast multi-elemental response, micro-destructiveness, instrumentation portability, have rendered it particularly suitable for analytical applications in the field of environmental science, space exploration and cultural heritage. This review reports and discusses LIBS achievements in these areas and results obtained for soils and aqueous samples, meteorites and terrestrial samples simulating extraterrestrial planets, and cultural heritage samples, including buildings and objects of various kinds.

  12. Laser Induced Breakdown Spectroscopy for Elemental Analysis in Environmental, Cultural Heritage and Space Applications: A Review of Methods and Results

    Directory of Open Access Journals (Sweden)

    Alessandro De Giacomo

    2010-08-01

    Full Text Available Analytical applications of Laser Induced Breakdown Spectroscopy (LIBS, namely optical emission spectroscopy of laser-induced plasmas, have been constantly growing thanks to its intrinsic conceptual simplicity and versatility. Qualitative and quantitative analysis can be performed by LIBS both by drawing calibration lines and by using calibration-free methods and some of its features, so as fast multi-elemental response, micro-destructiveness, instrumentation portability, have rendered it particularly suitable for analytical applications in the field of environmental science, space exploration and cultural heritage. This review reports and discusses LIBS achievements in these areas and results obtained for soils and aqueous samples, meteorites and terrestrial samples simulating extraterrestrial planets, and cultural heritage samples, including buildings and objects of various kinds.

  13. An Autonomous System for Experimental Evolution of Microbial Cultures: Test Results Using Ultraviolet-C Radiation and Escherichia Coli.

    Science.gov (United States)

    Ouandji, Cynthia; Wang, Jonathan; Arismendi, Dillon; Lee, Alonzo; Blaich, Justin; Gentry, Diana

    2017-01-01

    At its core, the field of microbial experimental evolution seeks to elucidate the natural laws governing the history of microbial life by understanding its underlying driving mechanisms. However, observing evolution in nature is complex, as environmental conditions are difficult to control. Laboratory-based experiments for observing population evolution provide more control, but manually culturing and studying multiple generations of microorganisms can be time consuming, labor intensive, and prone to inconsistency. We have constructed a prototype, closed system device that automates the process of directed evolution experiments in microorganisms. It is compatible with any liquid microbial culture, including polycultures and field samples, provides flow control and adjustable agitation, continuously monitors optical density (OD), and can dynamically control environmental pressures such as ultraviolet-C (UV-C) radiation and temperature. Here, the results of the prototype are compared to iterative exposure and survival assays conducted using a traditional hood, UV-C lamp, and shutter system.

  14. Analysis of bacterial culture results of two types of platelet products%两种血小板制品细菌培养结果分析

    Institute of Scientific and Technical Information of China (English)

    林栋; 崔四平; 苏婉兰; 陈宝婵; 叶柱江

    2014-01-01

    目的:探讨单采血小板与混合血小板在血液采集制备过程中细菌污染的危险程度,为血小板的采集制备过程中改良工艺,提高输血安全性提供可靠数据。方法采用生物梅里埃公司生产的BaeT/Alert 3D 全自动细菌培养仪及应用需氧瓶(BPA Culture Bottle)、厌氧瓶(BPN Culture Bottle)对5210例单采血小板,1653例混合血小板进行了细菌培养,细菌培养阳性者转种,转种阳性者进行细菌鉴定。结果在5210例单采血小板细菌培养经确定阳性6例,阳性率为1.15‰;1653例混合血小板中细菌培养经确定阳性10例,阳性率6.05‰。结论混合血小板细菌培养阳性率(6.05‰)高于单采血小板(1.15‰),两者比较差异有统计学意义(P<0.01);血小板细菌污染情况仍然严峻,应引起注意。%Objective To investigate the criticality of bacterial pollution in the blood collection and preparation process of single donor platelets and mixed platelets in order to provide reliable data for improving the technology in the collection and preparation process of platelets and improving the blood transfusion safety. Methods The BaeT/Alert 3D Full-Automatic Bacterial Culture System produced by BioMérieux and the BPA Culture Bottle and BPN Culture Bottle were used for the bacterial culture of 5210 cases of single donor platelets and 1653 cases of mixed platelets.The samples showing g positive bacterial culture results received subcultivation and the subcultivated sample showing positive results received bacterial identification. Results Of the 5210 cases of single donor platelets,bacterial culture confirmed 6 positive cases,with the positive rate of 1.15‰.Of the 1653 cases of mixed platelets,bacterial culture confirmed 10 positive cases,with the positive rate of 6.05‰. Conclusion The positive bacterial culture rate of mixed platelets (6.05‰)is higher than that of the single donor platelets (1.15

  15. Sustainability and the cultural factor. Results from the Dutch GOES Mass Public Module

    Energy Technology Data Exchange (ETDEWEB)

    Ester, P.; Vinken, H. [Institute for Globalization and Sustainable Development GLOBUS, Tilburg University, Tilburg (Netherlands)

    2000-07-01

    GOES stands for the GlObal Environmental Survey. The Dutch government declared sustainable development as the cornerstone of its environmental policy. This strategic policy shift presupposes a concerned citizenry willing to accept the way this might affect private lifestyles. The Dutch GOES MASS PUBLIC MODULE - part of a large-scale international study on citizens' environmental perceptions, values, and behaviors - shows the Dutch are concerned about environmental problems. Though they are most worried about classic environmental problems (e.g. air pollution), they realize that more long-term issues (e.g. global warming) are pressing too. They define themselves as being well-informed, feel the individual can make a difference, display a high self-efficacy, and prioritize ecological concerns over economic gains. They, however, do not accept policies that limit personal choice. The Dutch favor soft policies, voluntary lifestyle change, and softer ways of taking action (e.g. checkbook participation). Water and energy conservation no longer seem on the household agenda. Explaining policy preferences, protective action, and individual environmentally-friendly behavior shows environmental attitudes hardly make a difference. Basic values (e.g. post materialism), supporting the environment over economic growth, and a higher education do have an impact as regards protective action. This is less so for policy preferences or environmentally-friendly behavior. The Dutch GOES MASS PUBLIC MODULE points at the imperative necessity to bridge the grand environmental narratives of policy makers, particularly when they frame ecological problems as global, abstract, and long-term issues, and the here-and-now narratives of the well-informed and concerned citizens in their role of responsible consumers.

  16. Environmental change and cultural adaptation in the southern Caucasus: latest results from Aghitu-3 Cave, Armenia

    Science.gov (United States)

    Kandel, Andrew; Gasparyan, Boris; Bruch, Angela; Nahapetyan, Samvel; Weissbrod, Lior

    2014-05-01

    The well stratified locality of Aghitu-3 Cave in the southern Caucasus provides a glimpse into the daily lives of the earliest known Upper Paleolithic inhabitants of Armenia. With three main phases of sedimentation serving as a backdrop to human settlement, the locality provides an excellent stratigraphic record documenting environmental change between about 40,000 and 24,000 cal BP. From the end of MIS 3 with its warm and humid climate, early modern humans made use of the cave with increasing frequency during the colder and drier MIS 2. People produced stone tools from obsidian and chert, hunted medium sized ungulates and equids, and used bone tools to make clothing. The archaeological finds from Aghitu-3 suggest that mobile groups of Upper Paleolithic people used this high altitude (1601 m) cave as a seasonal camp with increasing frequency over time. Carnivores and birds of prey also made use of the cave, likely when humans were not present. The paleoenvironmental indicators confirm a warmer and more humid climate during the first sedimentary phase between about 40,000 and 33,000 cal BP. At about 35,000 cal BP the pollen profile indicates a vegetation of mixed deciduous and steppic species. The presence of green algae supports the interpretation that more humid conditions prevailed. Geological analysis indicates uniform, fine-grained deposition along the banks of a slow moving stream. This and the general lack of rock falls confirm a warmer and more humid climate. The spectrum of micromammals shows a higher proportion of golden hamster, also supporting a favorable climate. However, starting about 33,000 cal BP, we observe a second phase of deposition characterized by coarser sediment with evidence of rill washing, cycles of erosion and frequent large basalt rock falls. These observations suggest a dramatic cooling trend as the climate began to deteriorate. The third depositional phase starts about 29,000 cal BP and is marked by mainly aeolian deposition

  17. Growth and Survival Rate of Tilapia (Oreochromis niloticus Larvae Fed by Daphnia magna Cultured With Organic Fertilizer Resulted From Probiotic Bacteria Fermentation

    Directory of Open Access Journals (Sweden)

    Vivi Endar Herawati

    2015-10-01

    Full Text Available Daphnia magna is a potential feed for fish. The aim of this research was to find the best treatment and effect of D. magna culture addition from fermented organic fertilizer, to growth and survival rate of Oreochromis niloticus larvae. There were five treatments, each with three repetitions used in the study. All treatments used chicken dung, and different combinations of rice bran, coconut oilcake waste and tilapia larvae. Feeding on tilapia was given by ad libitum method for five times a day until 14 days. Water quality during the research was maintained at temperature 28–29°C, DO 0.3 ppm and pH 8.1–8.2. Observed variables include relative growth rate, survival rate, food consumption rate and water quality. Our results showed that D. magna cultured by fermented organic fertilizer for tilapia larvae (O. niloticus had high significant effect (p < 0.01 on the relative growth rate and survival rate. Treatment of D. magna cultured by 1.2 g/L chicken manure, 0.9 g/L rice bran and 0.3 g/L coconut oilcake showed the highest value on the relative growth rate (10.86%; survival rate (98.46% and food consumption at first week (106.43% and second week (152.76%.

  18. The cultural dissemination significance of TV reality show--Tang Poetry as example%浅析电视真人秀节目的文化传播意义--以《唐诗风云会》为例

    Institute of Scientific and Technical Information of China (English)

    陈丹

    2016-01-01

    “真人秀”节目从西方电视媒体开始,经历的高度集中的发展,以惊人的速度在中国电视上发展壮大。在当今中国真人秀节目高度密集的情况下,如何利用真人秀节目进行对外文化传播,传播中国传统文化。文章通过对陕西卫视《唐诗风云会》节目的分析,总结了节目成功的基本要素。%The "reality show" program starts from the Western TV media, has experienced a high concentration of development, with amazing speed in the development of China's television. Chinese reality show highly intensive, how to use the reality show to carry out foreign cultural communication, spread traditional Chinese culture. This paper analyzes the Shaanxi satelite TV's "Tang poetry" program, summarizes the basic elements of the success of the program.

  19. Telling, showing, showing off: a walking tour

    NARCIS (Netherlands)

    Bal, M.G.

    1992-01-01

    Outlines how New York City sets an image, using nature as a sign. Its very layout demonstrates the importance of a balanced intercourse between background and figure, between plan and details, between chaos and organization. Central Park, the domesticated preserve of nature-within-culture; Exhibits;

  20. Process for the lysis of a culture of lactic acid bacteria by means of a lysin, and uses of the resulting lysed culture

    NARCIS (Netherlands)

    Buist, Girbe; Venema, Gerard; Kok, Jan; Ledeboer, Aat M.

    1995-01-01

    The invention provides a process for the lysis of a culture of lactic acid bacteria, or a product containing such culture e.g. cheese, by means of a lysin through the in situ production of a homologous autolysin, or a heterologous autolysin obtainable from Gram-positive bacteria esp. from lactic aci

  1. Process for inhibiting the growth of a culture of lactic acid bacteria, and optionally lysing the bacterial cells, and uses of the resulting lysed culture

    NARCIS (Netherlands)

    Nauta, Arjen; Venema, Gerard; Kok, Jan; Ledeboer, Aat M.

    1995-01-01

    The invention provides a process for inhibiting the growth of a culture of lactic acid bacteria, or a product containing such culture e.g. a cheese product, in which in the cells of the lactic acid bacteria a holin obtainable from bacteriophages of Gram-positive bacteria, esp. from bacteriophages of

  2. The culture of cancer cell lines as tumorspheres does not systematically result in cancer stem cell enrichment.

    Directory of Open Access Journals (Sweden)

    Christophe Y Calvet

    Full Text Available Cancer stem cells (CSC have raised great excitement during the last decade and are promising targets for an efficient treatment of tumors without relapses and metastases. Among the various methods that enable to enrich cancer cell lines in CSC, tumorspheres culture has been predominantly used. In this report, we attempted to generate tumorspheres from several murine and human cancer cell lines: B16-F10, HT-29, MCF-7 and MDA-MB-231 cells. Tumorspheres were obtained with variable efficiencies from all cell lines except from MDA-MB-231 cells. Then, we studied several CSC characteristics in both tumorspheres and adherent cultures of the B16-F10, HT-29 and MCF-7 cells. Unexpectedly, tumorspheres-forming cells were less clonogenic and, in the case of B16-F10, less proliferative than attached cells. In addition, we did not observe any enrichment in the population expressing CSC surface markers in tumorspheres from B16-F10 (CD133, CD44 and CD24 markers or MCF-7 (CD44 and CD24 markers cells. On the contrary, tumorspheres culture of HT-29 cells appeared to enrich in cells expressing colon CSC markers, i.e. CD133 and CD44 proteins. For the B16-F10 cell line, when 1 000 cells were injected in syngenic C57BL/6 mice, tumorspheres-forming cells displayed a significantly lower tumorigenic potential than adherent cells. Finally, tumorspheres culture of B16-F10 cells induced a down-regulation of vimentin which could explain, at least partially, the lower tumorigenicity of tumorspheres-forming cells. All these results, along with the literature, indicate that tumorspheres culture of cancer cell lines can induce an enrichment in CSC but in a cell line-dependent manner. In conclusion, extensive characterization of CSC properties in tumorspheres derived from any cancer cell line or cancer tissue must be performed in order to ensure that the generated tumorspheres are actually enriched in CSC.

  3. Cultural universality and specificity of student engagement in school: The results of an international study from 12 countries.

    Science.gov (United States)

    Lam, Shui-fong; Jimerson, Shane; Shin, Hyeonsook; Cefai, Carmel; Veiga, Feliciano H; Hatzichristou, Chryse; Polychroni, Fotini; Kikas, Eve; Wong, Bernard P H; Stanculescu, Elena; Basnett, Julie; Duck, Robert; Farrell, Peter; Liu, Yi; Negovan, Valeria; Nelson, Brett; Yang, Hongfei; Zollneritsch, Josef

    2016-03-01

    A comprehensive understanding of the contextual factors that are linked to student engagement requires research that includes cross-cultural perspectives. This study investigated how student engagement in school is associated with grade, gender, and contextual factors across 12 countries. It also investigated whether these associations vary across countries with different levels of individualism and socio-economic development. The participants were 3,420 7th, 8th, and 9th grade students from Austria, Canada, China, Cyprus, Estonia, Greece, Malta, Portugal, Romania, South Korea, the United Kingdom, and the United States. The participants completed a questionnaire to report their engagement in school, the instructional practices they experienced, and the support they received from teachers, peers, and parents. Hierarchical linear modelling was used to examine the effects at both student and country levels. The results across countries revealed a decline in student engagement from Grade 7 to Grade 9, with girls reporting higher engagement than boys. These trends did not vary across the 12 countries according to the Human Development Index and Hofstede's Individualism Index. Most of the contextual factors (instructional practices, teacher support, and parent support) were positively associated with student engagement. With the exception that parent support had a stronger association with student engagement in countries with higher collectivism, most of the associations between the contextual factors and student engagement did not vary across countries. The results indicate both cultural universality and specificity regarding contextual factors associated with student engagement in school. They illustrate the advantages of integrating etic and emic approaches in cross-cultural investigations. © 2015 The British Psychological Society.

  4. EXPANDING URBAN AMERICAN INDIAN YOUTHS’ REPERTOIRE OF DRUG RESISTANCE SKILLS: PILOT RESULTS FROM A CULTURALLY ADAPTED PREVENTION PROGRAM

    Science.gov (United States)

    Kulis, Stephen; Dustman, Patricia A.; Brown, Eddie F.; Martinez, Marcos

    2013-01-01

    This article examines changes in the drug resistance strategies used by urban American Indian (UAI) middle school students during a pilot test of a substance use prevention curriculum designed specifically for UAI youth, Living in 2 Worlds (L2W). L2W teaches four drug resistance strategies (refuse, explain, avoid, leave [R-E-A-L]) in culturally appropriate ways. Data come from 57 UAI students (53% female; mean age = 12.5 years) who participated in L2W during an academic enrichment class for Native youth at two Phoenix schools. Students completed a pre-test questionnaire before the L2W lessons and a post-test 7 months later. Questions assessed the use of R-E-A-L and alternative strategies commonly reported by UAI youth (change the subject, use humor). Tests of mean differences from pre-test to post-test showed significant increases in use of refuse, R-E-A-L repertoire. Use of more passive strategies (avoid, use humor) did not change significantly, except for change the subject, which increased. Changes in the use of strategies did not differ significantly by gender, age, school grades, parental education, or length of urban residence. The L2W curriculum appears effective in teaching culturally relevant communication strategies that expand UAI youths’ repertoire of drug resistance skills. PMID:23529769

  5. Review of Deconversion: Qualitative and Quantitative Results from Cross-Cultural Research in Germany and the USA

    National Research Council Canada - National Science Library

    Andreea Nica

    2016-01-01

    ...: the active process by which individuals disaffiliate from religion. The 'Bielefeld-Based Cross-Cultural Study of Deconversion' consists of a mixed methods and cross-cultural approach to religious change in the USA and Germany...

  6. Culture and Psychiatric Symptoms in Puerto Rican Children: Longitudinal Results from One Ethnic Group in Two Contexts

    Science.gov (United States)

    Duarte, Cristiane S.; Bird, Hector R.; Shrout, Patrick E.; Wu, Ping; Lewis-Fernandez, Roberto; Shen, Sa; Canino, Glorisa

    2008-01-01

    Background: The development of youth psychopathology may be associated with direct and continuous contact with a different culture (acculturation) and to distress related to this process (cultural stress). We examine cultural experiences of Puerto Rican families in relation to youth psychiatric symptoms in two different contexts: one in which…

  7. Culture and Psychiatric Symptoms in Puerto Rican Children: Longitudinal Results from One Ethnic Group in Two Contexts

    Science.gov (United States)

    Duarte, Cristiane S.; Bird, Hector R.; Shrout, Patrick E.; Wu, Ping; Lewis-Fernandez, Roberto; Shen, Sa; Canino, Glorisa

    2008-01-01

    Background: The development of youth psychopathology may be associated with direct and continuous contact with a different culture (acculturation) and to distress related to this process (cultural stress). We examine cultural experiences of Puerto Rican families in relation to youth psychiatric symptoms in two different contexts: one in which…

  8. How many sputum culture results do we need to monitor multidrug-resistant-tuberculosis (MDR-TB) patients during treatment?

    Science.gov (United States)

    Janssen, Saskia; Padanilam, Xavier; Louw, Rianna; Mahanyele, Russel; Coetzee, Gerrit; Hänscheid, Thomas; Leenstra, Tjalling; Grobusch, Martin P

    2013-02-01

    Discharge of a hospital patient after a single negative sputum culture may save money when treating multidrug-resistant tuberculosis. However, after initial sputum conversion in 336 South Africans, 11.6% and 5.4% reconverted after 1 and 2 months, respectively. These findings endorse the WHO definitions of 2 negative cultures taken ≥ 30 days apart after sputum culture conversion.

  9. A yeast glycolipid biosurfactant, mannosylerythritol lipid, shows potential moisturizing activity toward cultured human skin cells: the recovery effect of MEL-A on the SDS-damaged human skin cells.

    Science.gov (United States)

    Morita, Tomotake; Kitagawa, Masaru; Suzuki, Michiko; Yamamoto, Shuhei; Sogabe, Atsushi; Yanagidani, Shusaku; Imura, Tomohiro; Fukuoka, Tokuma; Kitamoto, Dai

    2009-01-01

    Mannosylerythritol lipids (MELs) are produced in large amounts from renewable vegetable oils by Pseudozyma antarctica, and are the most promising biosurfactants known due to its versatile interfacial and biochemical actions. In order to broaden the application in cosmetics and pharmaceuticals, the skin care property of MEL-A, the major component of MELs, was investigated using a three-dimensional cultured human skin model. The skin cells were cultured and treated with sodium dodecyl sulfate (SDS) solution of 1 wt%, and the effects of different lipids on the SDS-damaged cells were then evaluated on the basis of the cell viability. The viability of the damaged cells was markedly recovered by the addition of MEL-A in a dose-dependent manner. Compared to the control, MEL-A solutions of 5 wt% and 10 wt% gave the recovery rate of 73% and 91%, respectively, while ceramide solution of 1 wt% gave the rate of over 100%. This revealed that MEL-A shows a ceramide-like moisturizing activity toward the skin cells. Considering the drawbacks of natural ceramides, namely limited amount and high production cost, the yeast biosurfactants should have a great potential as a novel moisturizer for treating the damaged skin.

  10. Integrated management of pearl culture in French Polynesia in the context of global change: Synopsis of existing results

    Science.gov (United States)

    Gueguen, Yannick; Gaertner-Mazouni, Nabila; Lo, Cedrik; Le Moullac, Gilles

    2016-12-01

    The paper presents a state of the art on knowledge of the black pearl oyster aquaculture at the scale of French Polynesia. It also introduces the main results obtained throughout the multi-partners POLYPERL project. The goal of this project was to develop an integrated and participative action-research of the pearl oyster culture system, focusing on the environmental, technological, economical and societal dimensions impacting the industry. The research proposed ranges from the understanding of biological phenomena of the production system to socio-economic aspects and governance of the industry, taking into account the management of anthropogenic, climate and health risks. We introduce here the different papers from the POLYPERL project that are compiled in this volume of ECSS dealing with recent scientific work that can contribute to sustainability of the pearl industry in French Polynesia.

  11. Heavy metal incorporation in foraminiferal calcite: results from multi-element enrichment culture experiments with Ammonia tepida

    Directory of Open Access Journals (Sweden)

    G.-J. Reichart

    2010-08-01

    Full Text Available The incorporation of heavy metals into carbonate tests of the shallow water benthic foraminifer Ammonia tepida was investigated under controlled laboratory conditions. Temperature, salinity, and pH of the culture solutions were kept constant throughout the duration of this experiment, while trace metal concentrations were varied. Concentrations of Ni, Cu, and Mn were set 5-, 10-, and 20 times higher than levels found in natural North Sea water; for reference, a control experiment with pure filtered natural North Sea water was also analysed. The concentrations of Cu and Ni from newly grown chambers were determined by means of both μ-synchrotron XRF and Laser Ablation Inductively Coupled Plasma Mass Spectroscopy (LA-ICP-MS. The results of both independent analytical techniques agreed within the analytical uncertainty. In general, the concentration of the analysed elements in the tests increased in line with their concentration in the culture solutions. Potential toxic and/or chemical competition effects might have resulted in the decreased incorporation of Ni and Cu into the calcite of the specimens exposed to the highest elemental concentrations. Mn incorporation exhibited large variability in the experiment with the 20-fold increased element concentrations, potentially due to antagonistic effects with Cu. The partition coefficients of Cu and Ni were calculated to be 0.14 ± 0.02 and 1.0 ± 0.5, respectively, whereas the partition coefficient of Mn was estimated to be least 2.4. These partition coefficients now open the way for reconstructing past concentrations for these elements in sea water.

  12. Patient Safety Culture in Nephrology Nurse Practice Settings: Results by Primary Work Unit, Organizational Work Setting, and Primary Role.

    Science.gov (United States)

    Ulrich, Beth; Kear, Tamara

    2015-01-01

    Patient safety culture is critical to the achievement of patient safety. In 2014, a landmark national study was conducted to investigate patient safety culture in nephrology nurse practice settings. In this secondary analysis of data from that study, we report the status of patient safety culture by primary work unit (chronic hemodialysis unit, acute hemodialysis unit, peritoneal dialysis unit) and organizational work setting (for-profit organization, not-for-profit organization), and compare the perceptions of direct care nurses and managers/administrators on components of patient safety culture.

  13. Shared cultural norms for justice in water institutions: results from Fiji, Ecuador, Paraguay, New Zealand, and the U.S.

    Science.gov (United States)

    Wutich, Amber; York, Abigail M; Brewis, Alexandra; Stotts, Rhian; Roberts, Christopher M

    2012-12-30

    It is widely agreed that current institutions are insufficient to meet global water challenges, and extensive institutional reforms are needed. To achieve effective local water management, institutional rules should be congruent with local cultural norms. Conversely, a major potential challenge is posed by tensions between institutional rules and local cultural norms for justice. We propose and demonstrate a new approach to cross-cultural analysis designed to investigate this tension, which can assess when local cultural norms are likely to facilitate or impede the acceptance of specific institutional rules. Using data from 238 respondents in five global sites (in Fiji, Ecuador, Paraguay, New Zealand, and the U.S.) analyzed using cultural consensus analysis, we find evidence of culturally-shared norms of justice in water institutions in at least six domains: a human right to water, water governance, water access, environmental stewardship, aspects of water markets, and aspects of water quality and health. Additionally, local cultural models across sites differed on only two topics: (1) ownership and allocation and (2) restrictions and enforcement. Indigenous heritage is the best single predictor of views on controversial institutional rules dealing with water restrictions/enforcement and ownership/allocation. This approach can help build effective water management solutions by identifying cases in which specific institutional reforms are congruent with local cultural norms (or not), and when those will matter most.

  14. Real life turnaround time of blood cultures in the clinical microbiology laboratory: results of the first Italian survey, May 2015

    Directory of Open Access Journals (Sweden)

    Fabio Arena

    2016-10-01

    Full Text Available Background and aims: Blood culture (BC results are essential to guide antimicrobial chemotherapy for patients with sepsis. However, BC is a time-consuming exam, which can take several days. Reducing BCs turn around time (TAT could impact on multiple outcome parameters and TAT monitoring is an important tool for measurement of microbiology laboratory performance. The aim of this study was to provide an overview of BC TATs among Italian microbiology laboratories. Materials and methods: Five laboratories collected and recorded, for a month period, date and time of the BC processing events. Cumulative TATs were analysed using the GraphPad software. Results: Participating laboratories reported data from 302 sepsis episodes. The median time from when the BC system produced a positive signal until Gram-stain results were reported was 7.6 hours. A rapid molecular identification and antimicrobial susceptibility testing (AST was performed in 26.5% of BCs. Mean TAT for identification report was significantly lower when a molecular approach was adopted (12 vs. 28.7 hours, P<0.001. Similarly, results of the molecular AST were obtained more than 24 hours in advance compared with phenotypic AST (mean 13.2 vs. 47.6, P<0.001. TATs from BC positivity of laboratories opened 7 days/week were not significantly lower than those of laboratories opened 6 days/week. Conclusions: BC is a time-consuming exam, however, molecular identification and AST methods can drastically reduce time to results. The lack of difference between TATs observed for laboratories working 7 days/week and 6 days/week, coupled with a high rate of BCs turning positive during the night enable to conclude that the most urgent measure to reduce TATs is the expansion of laboratory regular duty hours.

  15. Violence and TV Shows

    OpenAIRE

    ÖZTÜRK, Yrd. Doç. Dr. Şinasi

    2008-01-01

    This study aims to discuss theories on theviolent effects of TV shows on viewers, especiallyon children. Therefore, this study includes a briefdiscussion of definitions of violence, discussionof violence theories, main results of researcheson televised violence, measuring TV violence,perception of televised violence, individualdifferences and reactions to TV violence,aggressiveness and preferences for TV violence.

  16. Treasures on Show

    Institute of Scientific and Technical Information of China (English)

    YIN PUMIN

    2010-01-01

    @@ An exhibition of important relics and contemporary art pieces of China was held in Beijing on October 21-24,idisplaying the country's rich culture and its boundless charm. As the largest Chinese antique art fair,the Second Beijing China Art International Fair (BCAIF) offered a good opportunity for people to enjoy hundreds of the best and rare Chinese art treasures, said Yu Ping,Deputy Director of the Beijing Municipal Administration of Cultural Heritage, one of the sponsors of the fair.

  17. Benthic foraminifera cultured over a large salinity gradient: first results and comparison with field data from the Baltic Sea.

    Science.gov (United States)

    Groeneveld, Jeroen; Filipsson, Helena L.; Austin, William E. N.; Darling, Kate; Quintana Krupinski, Nadine B.

    2015-04-01

    Some of the most significant challenges in paleoclimate research arise from the need to both understand and reduce the uncertainty associated with proxy methods for climate reconstructions. This is especially important for shelf and coastal environments where increasing numbers of high-resolution paleorecords are being generated. These challenges are further highlighted in connection with ECORD/IODP Expedition 347: Baltic Sea Paleoenvironments. This large-scale drilling operation took place in the Baltic Sea region during the autumn of 2013. At this time, there is a pressing need for proxy calibrations directly targeted at the brackish Baltic environment. Within the CONTEMPORARY project we are investigating different temperature and salinity proxy variables through a combination of field- and culture-based benthic foraminiferal samples, together with genetic characterization (genotyping) of the morphospecies. We have completed two field campaigns where we collected (living) foraminifera and water samples at several sites, ranging from fully marine to low salinity conditions. The core-top foraminifera have been analysed for trace metal/Ca, stable oxygen and carbon isotopes, and faunal composition. Living foraminifera collected from the sediment-water interface were cultured in sea water in two long-term experiments at different temperatures (5°C and 10°C) and at three different salinities (15, 25, and 35). The first experiment yielded a large number of reproduced and experimentally-grown Elphidium specimens. The second experiment resulted in growth but no reproduction. We will provide a summary of the experimentally grown material and discuss the challenges of generating new proxy calibrations for foraminiferal shell geochemistry in the Baltic Sea. Furthermore, specimens of Elphidium and Ammonia, found at two sampling sites (Anholt, Kattegat and Hanöbay) with differing salinities, were genotyped and the results indicate that the same genotype of Elphidium is

  18. Can We Reduce Negative Blood Cultures With Clinical Scores and Blood Markers? Results From an Observational Cohort Study

    Science.gov (United States)

    Laukemann, Svenja; Kasper, Nina; Kulkarni, Prasad; Steiner, Deborah; Rast, Anna Christina; Kutz, Alexander; Felder, Susan; Haubitz, Sebastian; Faessler, Lukas; Huber, Andreas; Fux, Christoph A.; Mueller, Beat; Schuetz, Philipp

    2015-01-01

    Abstract Only a small proportion of blood cultures routinely performed in emergency department (ED) patients is positive. Multiple clinical scores and biomarkers have previously been examined for their ability to predict bacteremia. Conclusive clinical validation of these scores and biomarkers is essential. This observational cohort study included patients with suspected infection who had blood culture sampling at ED admission. We assessed 5 clinical scores and admission concentrations of procalcitonin (PCT), C-reactive protein (CRP), lymphocyte and white blood cell counts, the neutrophil-lymphocyte count ratio (NLCR), and the red blood cell distribution width (RDW). Two independent physicians assessed true blood culture positivity. We used logistic regression models with area under the curve (AUC) analysis. Of 1083 patients, 104 (9.6%) had positive blood cultures. Of the clinical scores, the Shapiro score performed best (AUC 0.729). The best biomarkers were PCT (AUC 0.803) and NLCR (AUC 0.700). Combining the Shapiro score with PCT levels significantly increased the AUC to 0.827. Limiting blood cultures only to patients with either a Shapiro score of ≥4 or PCT > 0.1 μg/L would reduce negative sampling by 20.2% while still identifying 100% of positive cultures. Similarly, a Shapiro score ≥3 or PCT >0.25 μg/L would reduce cultures by 41.7% and still identify 96.1% of positive blood cultures. Combination of the Shapiro score with admission levels of PCT can help reduce unnecessary blood cultures with minimal false negative rates. The study was registered on January 9, 2013 at the ‘ClinicalTrials.gov’ registration web site (NCT01768494). PMID:26656373

  19. Can We Reduce Negative Blood Cultures With Clinical Scores and Blood Markers? Results From an Observational Cohort Study.

    Science.gov (United States)

    Laukemann, Svenja; Kasper, Nina; Kulkarni, Prasad; Steiner, Deborah; Rast, Anna Christina; Kutz, Alexander; Felder, Susan; Haubitz, Sebastian; Faessler, Lukas; Huber, Andreas; Fux, Christoph A; Mueller, Beat; Schuetz, Philipp

    2015-12-01

    Only a small proportion of blood cultures routinely performed in emergency department (ED) patients is positive. Multiple clinical scores and biomarkers have previously been examined for their ability to predict bacteremia. Conclusive clinical validation of these scores and biomarkers is essential.This observational cohort study included patients with suspected infection who had blood culture sampling at ED admission. We assessed 5 clinical scores and admission concentrations of procalcitonin (PCT), C-reactive protein (CRP), lymphocyte and white blood cell counts, the neutrophil-lymphocyte count ratio (NLCR), and the red blood cell distribution width (RDW). Two independent physicians assessed true blood culture positivity. We used logistic regression models with area under the curve (AUC) analysis.Of 1083 patients, 104 (9.6%) had positive blood cultures. Of the clinical scores, the Shapiro score performed best (AUC 0.729). The best biomarkers were PCT (AUC 0.803) and NLCR (AUC 0.700). Combining the Shapiro score with PCT levels significantly increased the AUC to 0.827. Limiting blood cultures only to patients with either a Shapiro score of ≥4 or PCT > 0.1 μg/L would reduce negative sampling by 20.2% while still identifying 100% of positive cultures. Similarly, a Shapiro score ≥3 or PCT >0.25 μg/L would reduce cultures by 41.7% and still identify 96.1% of positive blood cultures.Combination of the Shapiro score with admission levels of PCT can help reduce unnecessary blood cultures with minimal false negative rates.The study was registered on January 9, 2013 at the 'ClinicalTrials.gov' registration web site (NCT01768494).

  20. Culture of mouse amniotic fluid-derived cells on irradiated STO feeders results in the generation of primitive endoderm cell lines capable of self-renewal in vitro.

    Science.gov (United States)

    Babic, Aleksandar M; Jang, Sunyoung; Nicolov, Eugenia; Voicu, Horatiu; Luckey, Chance J

    2013-01-01

    The cells present in amniotic fluid (AF) are currently used for prenatal diagnosis of fetal anomalies but are also a potential source of cells for cell therapy. To better characterize putative progenitor cell populations present in AF, we used culture conditions that support self-renewal to determine if these promoted the generation of stable cell lines from AF-derived cells (AFC). Cells isolated from E11.5 mouse were cultured on irradiated STO fibroblast feeder layers in human embryonic germ cell derivation conditions. The cultures grew multicellular epithelial colonies that could be repropagated from single cells. Reverse transcription semiquantitative polymerase chain reaction of established cell lines revealed that they belonged to the extraembryonic endoderm (ExEn) expressing high levels of Gata6, Gata4, Sox17, Foxa2 and Sox7 mRNA. Hierarchical clustering based on the whole transcriptome expression profile of the AFC lines (AFCL) shows significant correlation between transcription profiles of AFCL and blastocyst-derived XEN, an ExEn cell line. In vitro differentiation of AFCL results in the generation of cells expressing albumin and α-fetoprotein (AFP), while intramuscular injection of AFCL into immunodeficient mice produced AFP-positive tumors with primitive endodermal appearance. Hence, E11.5 mouse AF contains cells that efficiently produce XEN lines. These AF-derived XEN lines do not spontaneously differentiate into embryonic-type cells but are phenotypically stable and have the capacity for extensive expansion. The lack of requirement for reprogramming factors to turn AF-derived progenitor cells into stable cell lines capable of massive expansion together with the known ability of ExEn to contribute to embryonic tissue suggests that this cell type may be a candidate for banking for cell therapies. © 2013 S. Karger AG, Basel

  1. Nutritional value content, biomass production and growth performance of Daphnia magna cultured with different animal wastes resulted from probiotic bacteria fermentation

    Science.gov (United States)

    Endar Herawati, Vivi; Nugroho, R. A.; Pinandoyo; Hutabarat, Johannes

    2017-02-01

    Media culture is an important factor for the growth and quality of Daphnia magna nutrient value. This study has purpose to find the increasing of nutritional content, biomass production and growth performance of D. magna using different animal wastes fermented by probiotic bacteria. This study conducted using completely randomized experimental design with 10 treatments and 3 replicates. Those media used different animal manures such as chicken manure, goat manure and quail manure mixed by rejected bread and tofu waste fermented by probiotic bacteria then cultured for 24 days. The results showed that the media which used 50% chicken manure, 100% rejected bread and 50% tofu waste created the highest biomass production, population and nutrition content of D.magna about 2111788.9 ind/L for population; 342 grams biomass production and 68.85% protein content. The highest fatty acid profile is 6.37% of linoleic and the highest essential amino acid is 22.8% of lysine. Generally, the content of ammonia, DO, temperature, and pH during the study were in the good range of D. magna’s life. This research has conclusion that media used 50% chicken manure, 100% rejected bread and 50% tofu waste created the highest biomass production, population and nutrition content of D. magna.

  2. A culture-based calibration of benthic foraminiferal paleotemperature proxies: δ18O and Mg/Ca results

    Science.gov (United States)

    Filipsson, H. L.; Bernhard, J. M.; Lincoln, S. A.; McCorkle, D. C.

    2010-04-01

    Benthic foraminifera were cultured for five months at four temperatures (4, 7, 14 and 21 °C) to establish the temperature dependence of foraminiferal calcite δ18O and Mg/Ca. Two Bulimina species (B. aculeata and B. marginata) were most successful in terms of calcification, adding chambers at all four temperatures and reproducing at 7 and 14 °C. Foraminiferal δ18O values displayed ontogenetic variations, with lower values in younger individuals. The δ18O values of adult specimens decreased with increasing temperature in all but the 4 °C treatment, exhibiting a relationship consistent with previous δ18O paleotemperature calibration studies. Foraminiferal Mg/Ca values, determined by laser ablation inductively coupled plasma mass spectrometry, were broadly consistent with previous Mg/Ca calibration studies, but extremely high values in the 4 °C treatment and higher than predicted values at two of the other three temperatures make it challenging to interpret these results.

  3. Education and guidance to the civil protection culture: insights and first results from the SicuraMente project

    Science.gov (United States)

    Romeo, Saverio; Benvenuti, Eugenio; Calabrese, Danilo; Galluccio, Dario Giuseppe; Tocci, Mauro

    2017-04-01

    To gain awareness on natural hazards social and educational initiatives are required, aimed at promoting a civil protection culture able to guide towards conscious and correct behavior. People, well-informed about risks in their territory, are safer citizens. In this perspective, it is important that the learning environments, such as schools and universities, play a key role on promoting dialogue and interactions among institutions, becoming a useful resource for the local community. In this framework, the present work describes an experimental project, called SicuraMente, started 2 years ago, about safety and civil protection culture coordinated by LARES Italia, a national civil protection association. This ongoing project, that involves high school and university students, is a training course that teaches skills and grants its participants specific toolset to increase the civic awareness and personal knowledge on actions to take in specific emergency situations. SicuraMente is a multi-hazard project, concerning several natural phenomena such as earthquake, flood, landslide, fire and extreme meteorological events. Each topic is discussed with a global approach starting from the scientific knowledge of the phenomenon, with a final discussion on the best practices about disaster prevention and preparedness. Additionally, in order to encourage the interest of students, 4 contexts are taken in example (home, school, city, society) with special case study sessions of each, with open discussion and debate. The project's activities are conducted on two levels: peer education lessons with "learning by doing" approach (e.g. simulation-based learning) under the coordination of LARES volunteers, and a further training stage at home in e-learning environment (www.sicuramentelab.it). In the first year of the project, 669 students from 9 schools of Regione Umbria (Central Italy) were involved. Thanks to the fundamental support of the volunteers of LARES association, 543

  4. Actionable Cultural Understanding for Support to Tactical Operations (ACUSTO): The Effect of Data Quality on Spatial Analysis Results

    Science.gov (United States)

    2009-06-01

    intelligence analysis ( Satyanarayana and Yogendran 2009), including socio-cultural factors are all with in the realm of GIS. These applications are... Satyanarayana , P., and S. Yogendran. 2009. Military Applications of GIS. IIC Technologies Private Limited, Hyderabad, India, Unpublished Manuscript

  5. Continuous Passaging of a Recombinant C-Strain Virus in PK-15 Cells Selects Culture-Adapted Variants that Showed Enhanced Replication but Failed to Induce Fever in Rabbits.

    Science.gov (United States)

    Tong, Chao; Chen, Ning; Liao, Xun; Yuan, Xuemei; Sun, Mengjiao; Li, Xiaoliang; Fang, Weihuan

    2017-09-28

    Classical swine fever virus (CSFV) is the etiologic agent of classical swine fever, a highly contagious disease that causes significant economic losses to the swine industry. The lapinized C-strain, a widely used vaccine strain against CSFV, has low growth efficiency in cell culture, which limits the productivity in the vaccine industry. In this study, a recombinant virus derived from C-strain was constructed and subjected to continuous passaging in PK-15 cells with the goal of acquiring a high progeny virus yield. A cell-adapted virus variant, RecCpp80, had nearly 1,000-fold higher titer than its parent C-strain but lost the ability to induce fever in rabbits. Sequence analysis of cell-adapted RecC variants indicated that at least six nucleotide changes were fixed in RecCpp80. Further adaption of RecCpp80 variant in swine testicle cells led to a higher virus yield without additional mutations. Introduction of each of these residues into the wild-type RecC backbone showed that one mutation, M979R (T3310G), located in the C-terminal region of E2 might be closely related to the cell-adapted phenotype. Rabbit inoculation revealed that RecCpp80+10 failed to induce fever in rabbits, whereas RecCpp40+10 caused a fever response similar to the commercial C-strain vaccine. In conclusion, the C-strain can be adapted to cell culture by introducing specific mutations in its E2 protein. The mutations in RecCpp80 that led to the loss of fever response in rabbits require further investigation. Continuous passaging of the C-strain-based recombinant viruses in PK-15 cells could enhance its in vitro adaption. The non-synonymous mutations at 3310 and 3531 might play major roles in the enhanced capacity of general virus reproduction. Such findings may help design a modified C-strain for improved productivity of commercial vaccines at reduced production cost.

  6. Class size and academic results, with a focus on children from culturally, linguistically and economically disenfranchised communities

    Directory of Open Access Journals (Sweden)

    David Zyngier

    2014-03-01

    Full Text Available The question of class size continues to attract the attention of educational policymakers and researchers alike. Australian politicians and their advisers, policy makers and political commentators agree that much of Australia’s increased expenditure on education in the last 30 years has been ‘wasted’ on efforts to reduce class sizes. They conclude that funding is therefore not the problem in Australian education, arguing that extra funding has not led to improved academic results. Many scholars have found serious methodological issues with the existing reviews that make claims for the lack of educational and economic utility in reducing class sizes in schools. Significantly, the research supporting the current policy advice to both state and federal ministers of education is highly selective, and based on limited studies originating from the USA. This comprehensive review of 112 papers from 1979-2014 assesses whether these conclusions about the effect of smaller class sizes still hold. The review draws on a wider range of studies, starting with Australian research, but also includes similar education systems such as England, Canada, New Zealand and non-English speaking countries of Europe. The review assesses the different measures of class size and how they affect the results, and also whether other variables such as teaching methods are taken into account. Findings suggest that smaller class sizes in the first four years of school can have an important and lasting impact on student achievement, especially for children from culturally, linguistically and economically disenfranchised communities. This is particularly true when smaller classes are combined with appropriate teacher pedagogies suited to reduced student numbers. Suggested policy recommendations involve targeted funding for specific lessons and schools, combined with professional development of teachers. These measures may help to address the inequality of schooling and

  7. Treasures on Show

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The international art fair provides an opportunity to build Beijing into an artwork trading center An exhibition of important relics and contemporary art pieces of China was held in Beijing on October 21-24, displaying the country’s rich culture and its boundless charm.

  8. Deficits in knowledge, attitude, and practice towards blood culture sampling: results of a nationwide mixed-methods study among inpatient care physicians in Germany.

    Science.gov (United States)

    Raupach-Rosin, Heike; Duddeck, Arne; Gehrlich, Maike; Helmke, Charlotte; Huebner, Johannes; Pletz, Mathias W; Mikolajczyk, Rafael; Karch, André

    2017-08-01

    Blood culture (BC) sampling rates in Germany are considerably lower than recommended. Aim of our study was to assess knowledge, attitudes, and practice of physicians in Germany regarding BC diagnostics. We conducted a cross-sectional mixed-methods study among physicians working in inpatient care in Germany. Based on the results of qualitative focus groups, a questionnaire-based quantitative study was conducted in 2015-2016. In total, 706 medical doctors and final-year medical students from 11 out of 16 federal states in Germany participated. BC sampling was considered an important diagnostic tool by 95% of the participants. However, only 23% of them would collect BCs in three scenarios for which BC ordering is recommended by present guidelines in Germany; almost one out of ten physicians would not have taken blood cultures in any of the three scenarios. The majority of participants (74%) reported not to adhere to the guideline recommendation that blood culture sampling should include at least two blood culture sets from two different injection sites. High routine in blood culture sampling, perceived importance of blood culture diagnostics, the availability of an in-house microbiological lab, and the department the physician worked in were identified as predictors for good blood culture practice. Our study suggests that there are substantial deficits in BC ordering and the application of guidelines for good BC practice in Germany. Based on these findings, multimodal interventions appear necessary for improving BC diagnostics.

  9. Using a participatory action strategic approach to enhance accessibility and participation in arts and cultural events: results of four focus groups.

    Science.gov (United States)

    Milligan, Nancy Vandewiele; Nieuwenhuijsen, Els R; Grawi, Carolyn L

    2014-01-01

    Cultural events are abundant in a midwestern college town; however, individuals with disabilities have expressed concerns about their accessibility. Policymakers, business owners, and managers often ignore disability-related issues. Research shows accessibility is the main environmental barrier to participation in arts and cultural events. Individuals with disabilities are disconnected from managers of cultural organizations and city leaders. The lack of awareness about accessibility, including access to the built environment, impedes participation in cultural events in this college town. To encourage the participation of people with disabilities in cultural events in a midwestern college town, a bold strategic project was initiated to conduct a community-based needs assessment as a foundation for an action plan. Participation in arts and culture was selected as a unique focal point for exploring ways to enhance accessibility. Thirty-nine stakeholders participated in four different focus groups: individuals with disabilities, managers of cultural organizations, caregivers and health care providers, and other stakeholders including politicians. Critical problem areas identified were mapped onto the environmental factors in the International Classification of Functioning, Disability, and Health (ICF). Three themes emerged: 1) limited awareness about accessibility among the residents with disabilities and a lack of awareness about disability-related issues and accessibility among the managers of cultural organizations; 2) the need for a "central information clearinghouse" to share, provide, and retrieve information; 3) the need for inclusive city-level policies. Raising awareness about disabilities and accessibility, providing a clearinghouse for information sharing and implementing inclusive policies are crucial to strengthen participation in community life. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Deaf Adults' Reasons for Genetic Testing Depend on Cultural Affiliation: Results from a Prospective, Longitudinal Genetic Counseling and Testing Study

    Science.gov (United States)

    Boudreault, Patrick; Baldwin, Erin E.; Fox, Michelle; Dutton, Loriel; Tullis, LeeElle; Linden, Joyce; Kobayashi, Yoko; Zhou, Jin; Sinsheimer, Janet S.; Sininger, Yvonne; Grody, Wayne W.; Palmer, Christina G. S.

    2010-01-01

    This article examines the relationship between cultural affiliation and deaf adults' motivations for genetic testing for deafness in the first prospective, longitudinal study to examine the impact of genetic counseling and genetic testing on deaf adults and the deaf community. Participants (n = 256), classified as affiliating with hearing, Deaf,…

  11. Cultural Universality and Specificity of Student Engagement in School: The Results of an International Study from 12 Countries

    Science.gov (United States)

    Lam, Shui-fong; Jimerson, Shane; Shin, Hyeonsook; Cefai, Carmel; Veiga, Feliciano H.; Hatzichristou, Chryse; Polychroni, Fotini; Kikas, Eve; Wong, Bernard P. H.; Stanculescu, Elena; Basnett, Julie; Duck, Robert; Farrell, Peter; Liu, Yi; Negovan, Valeria; Nelson, Brett; Yang, Hongfei; Zollneritsch, Josef

    2016-01-01

    Background: A comprehensive understanding of the contextual factors that are linked to student engagement requires research that includes cross-cultural perspectives. Aims: This study investigated how student engagement in school is associated with grade, gender, and contextual factors across 12 countries. It also investigated whether these…

  12. Assessing safety culture in NICU: psychometric properties of the Italian version of Safety Attitude Questionnaire and result implications.

    Science.gov (United States)

    Zenere, Alessandra; Zanolin, M Elisabetta; Negri, Roberta; Moretti, Francesca; Grassi, Mario; Tardivo, Stefano

    2016-04-01

    Neonatal intensive care units (NICUs) are a high-risk setting. The Safety Attitude Questionnaire (SAQ) is a widely used tool to measure safety culture. The aims of the study are to verify the psychometric properties of the Italian version of SAQ, to evaluate safety culture in the NICUs and to identify improvement interventions. A cross-sectional study was conducted in 6 level III NICUs. The SAQ was translated into Italian and adapted to the context, a confirmatory factor analysis (CFA) was performed to validate the questionnaire. 193 questionnaires were collected. The mean response rate was 59.7% (range 44.5%-95.7%). The answers were analysed according to six factors: f1 - teamwork climate, f2 - safety climate, f3 - job satisfaction, f4 - stress recognition, f5 - perception of management, f6 - working conditions. The CFA indexes were adequate (McDonald's omega indexes varied from 0.74 to 0.94, the SRMR index was equal to 0.79 and the RMSEA index was 0.070, 95% CI = 0.063-0.078). The mean composite score was 57.6 (SD 17.9), ranging between 42.3 and 69.7 on a standardized 100-point scale. We highlighted significant differences among units and professions (P tool to evaluate and compare the safety culture in the NICUs. The obtained scores significantly varied both within and among the NICUs. The organizational and structural characteristics of the involved hospitals probably affect the safety culture perception by the staff. © 2015 John Wiley & Sons, Ltd.

  13. Deaf Adults’ Reasons for Genetic Testing Depend on Cultural Affiliation: Results From a Prospective, Longitudinal Genetic Counseling and Testing Study

    OpenAIRE

    Boudreault, Patrick; Baldwin, Erin E.; Fox, Michelle; Dutton, Loriel; Tullis, LeeElle; Linden, Joyce; Kobayashi, Yoko; Zhou, Jin; Sinsheimer, Janet S.; Sininger, Yvonne; Grody, Wayne W.; Palmer, Christina G. S.

    2010-01-01

    This article examines the relationship between cultural affiliation and deaf adults’ motivations for genetic testing for deafness in the first prospective, longitudinal study to examine the impact of genetic counseling and genetic testing on deaf adults and the deaf community. Participants (n = 256), classified as affiliating with hearing, Deaf, or both communities, rated interest in testing for 21 reasons covering 5 life domains. Findings suggest strong interest in testing to learn why they ...

  14. Public medical shows.

    Science.gov (United States)

    Walusinski, Olivier

    2014-01-01

    In the second half of the 19th century, Jean-Martin Charcot (1825-1893) became famous for the quality of his teaching and his innovative neurological discoveries, bringing many French and foreign students to Paris. A hunger for recognition, together with progressive and anticlerical ideals, led Charcot to invite writers, journalists, and politicians to his lessons, during which he presented the results of his work on hysteria. These events became public performances, for which physicians and patients were transformed into actors. Major newspapers ran accounts of these consultations, more like theatrical shows in some respects. The resultant enthusiasm prompted other physicians in Paris and throughout France to try and imitate them. We will compare the form and substance of Charcot's lessons with those given by Jules-Bernard Luys (1828-1897), Victor Dumontpallier (1826-1899), Ambroise-Auguste Liébault (1823-1904), Hippolyte Bernheim (1840-1919), Joseph Grasset (1849-1918), and Albert Pitres (1848-1928). We will also note their impact on contemporary cinema and theatre.

  15. 28292份临床血液标本细菌培养结果%Bacterial culture results of 28 292 clinical blood specimens

    Institute of Scientific and Technical Information of China (English)

    孔繁林; 储从家; 管新龙; 李杰芬; 杨宇溪

    2011-01-01

    Objective To investigate the distribution characteristics of pathogens isolated from clinical blood specimens from a hospital. Methods Bacteria isolated from 55 606 bottles of 28 292 blood specimens between 1999 and 2008 were cultured and identified by mimi VITAL Blood Culture Automation System and Bact/ALERT 3D System and VITEK 32 Automicrobic System,and the culture result was analysed statistically. Results In aerobic and anaerobic culture of 28 292 blood specimens,5 837 showed positive culture results, the positive rate was 20. 63%;Thepositive rate of aerobic and anaerobic bacteria was 83. 50%(4 874/5 837) and 82. 30%(4 804/5 837)respectively. A total of 5 837 strains of 117 species of 43 genus were detected, Salmonella paratyphi A accounted for 4 486 stains (76. 85%). The distribution showed three peaks of bacterial detection were in the year of 2001 (1 263 strains) ,2004 (740 strains) and 2006 (713 strains). Gram-positive cocci were the main isolated bacteria , the ratio of grampositive cocci to gram-negative bacilli and fungi was 65. 73: 25. 30: 2. 74. Coagulase negative Staphylococcus (CNS) accounted for 37. 46% (493/1 316) of the detected bacteria except Salmonella spp. , and had a tendency of increasing gradually(x2 = 127. 81, P<0. 01 ). The isolation rate of fungus was increasing gradually(x2 = 29. 77, P<0. 01). The obligate anaerobes accounted for 0. 38%(22/5 837) of all isolated bacteria , and accounted for 1.67%(22/1 316) except Salmonella. Conclusion Performing both aerobic and anaerobic culture can enhance the positive detection rate. Constant detection of Salmonella paratyphi A and increasing tendency of opportunistic pathogens (CNS and fungus) should be paid much attention to.%目的 调查某院临床送检血液标本中病原菌的分布特征.方法 采用mimi VITAl全自动荧光血培养仪、Bact/ALERT 3D培养仪和VITEK32自动细菌鉴定仪对该院1999--2008年临床送检的28 292人份55 606瓶血标本进行培养

  16. Seeking Opportunities at The Show

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    There are four numbers that can describe a city, 7300 years of ancestor culture, 4300 years of city culture, 2700 years the canal culture, and 130 years of industrial culture. Another set of four numbers are: as the Chinese Knitting Culture

  17. The"showing"of traditional culture elements in outstanding photography work%刍议优秀摄影作品中传统文化元素的“流露”

    Institute of Scientific and Technical Information of China (English)

    侯承

    2013-01-01

    The photography can be regarded as the record of life, nature and scene, is the practice of a combination of art and the reality. For a superb photographer, not only pursue techniques and skills of photography, but also pursue artistic charm and cultural temperament of photography work. So, real excellent photography, many are the traditional cultural enlightenment. For many outstanding works of our country, including fine art, nor the lack of standards and traditional culture works. This paper discusses it as the clue, elaborates the revealing of traditional cultural element in present outstanding photographic works.%摄影可以看做是对生活、自然和景象的记录,是一种现实与艺术相结合的实践行为。对于高超的摄影师而言,不但要追求摄影的技巧和技艺,更要追求摄影作品的艺术魅力和文化气质。所以,真正优秀的摄影作品,很多都是受到了传统文化的启示。对于我国的很多优秀摄影作品来说,其中不乏艺术精品,也不缺少与传统文化接轨的作品。本文以此为论述线索,阐述了优秀摄影作品中传统文化元素的展现和流露。

  18. [Increase in the production of oncovirus type C by an L929 cell culture as a result of BCG infection].

    Science.gov (United States)

    Klitsunova, N V; Gosteva, V V; Kim, A A; Bykovskiĭ, A F

    1984-01-01

    The effect of BCG infection of L929 cells on replication of oncovirus type C was studied. Ultrathin sections of the BCG-infected culture were examined electron microscopically 1, 3, 6, 8, and 10 days postinfection. Most microorganisms with the morphology typical of mycobacteria were found inside phagosomes. The number of extracellular virions as well as budding and abnormal forms per one cell contour was counted. BCG-infected cells were found to produce significantly more virus than the controls. The difference was maximal 3 days postinoculation. Possible reasons for the increased oncovirus production by continuous cell lines after infection with BCG are discussed.

  19. The Great Cometary Show

    Science.gov (United States)

    2007-01-01

    The ESO Very Large Telescope Interferometer, which allows astronomers to scrutinise objects with a precision equivalent to that of a 130-m telescope, is proving itself an unequalled success every day. One of the latest instruments installed, AMBER, has led to a flurry of scientific results, an anthology of which is being published this week as special features in the research journal Astronomy & Astrophysics. ESO PR Photo 06a/07 ESO PR Photo 06a/07 The AMBER Instrument "With its unique capabilities, the VLT Interferometer (VLTI) has created itself a niche in which it provide answers to many astronomical questions, from the shape of stars, to discs around stars, to the surroundings of the supermassive black holes in active galaxies," says Jorge Melnick (ESO), the VLT Project Scientist. The VLTI has led to 55 scientific papers already and is in fact producing more than half of the interferometric results worldwide. "With the capability of AMBER to combine up to three of the 8.2-m VLT Unit Telescopes, we can really achieve what nobody else can do," added Fabien Malbet, from the LAOG (France) and the AMBER Project Scientist. Eleven articles will appear this week in Astronomy & Astrophysics' special AMBER section. Three of them describe the unique instrument, while the other eight reveal completely new results about the early and late stages in the life of stars. ESO PR Photo 06b/07 ESO PR Photo 06b/07 The Inner Winds of Eta Carinae The first results presented in this issue cover various fields of stellar and circumstellar physics. Two papers deal with very young solar-like stars, offering new information about the geometry of the surrounding discs and associated outflowing winds. Other articles are devoted to the study of hot active stars of particular interest: Alpha Arae, Kappa Canis Majoris, and CPD -57o2874. They provide new, precise information about their rotating gas envelopes. An important new result concerns the enigmatic object Eta Carinae. Using AMBER with

  20. Interspecies interactions result in enhanced biofilm formation by co-cultures of bacteria isolated from a food processing environment.

    Science.gov (United States)

    Røder, Henriette L; Raghupathi, Prem K; Herschend, Jakob; Brejnrod, Asker; Knøchel, Susanne; Sørensen, Søren J; Burmølle, Mette

    2015-10-01

    Bacterial attachment and biofilm formation can lead to poor hygienic conditions in food processing environments. Furthermore, interactions between different bacteria may induce or promote biofilm formation. In this study, we isolated and identified a total of 687 bacterial strains from seven different locations in a meat processing environment and evaluated their biofilm formation capability. A diverse group of bacteria was isolated and most were classified as poor biofilm producers in a Calgary biofilm device assay. Isolates from two sampling sites, the wall and the meat chopper, were further examined for multispecies biofilm formation. Eight strains from each sampling site were chosen and all possible combinations of four member co-cultures were tested for enhanced biofilm formation at 15 °C and 24 °C. In approximately 20% of the multispecies consortia grown at 15 °C, the biofilm formation was enhanced when comparing to monospecies biofilms. Two specific isolates (one from each location) were found to be present in synergistic combinations with higher frequencies than the remaining isolates tested. This data provides insights into the ability of co-localized isolates to influence co-culture biofilm production with high relevance for food safety and food production facilities.

  1. Oxygen glucose deprivation in rat hippocampal slice cultures results in alterations in carnitine homeostasis and mitochondrial dysfunction.

    Directory of Open Access Journals (Sweden)

    Thomas F Rau

    Full Text Available Mitochondrial dysfunction characterized by depolarization of mitochondrial membranes and the initiation of mitochondrial-mediated apoptosis are pathological responses to hypoxia-ischemia (HI in the neonatal brain. Carnitine metabolism directly supports mitochondrial metabolism by shuttling long chain fatty acids across the inner mitochondrial membrane for beta-oxidation. Our previous studies have shown that HI disrupts carnitine homeostasis in neonatal rats and that L-carnitine can be neuroprotective. Thus, this study was undertaken to elucidate the molecular mechanisms by which HI alters carnitine metabolism and to begin to elucidate the mechanism underlying the neuroprotective effect of L-carnitine (LCAR supplementation. Utilizing neonatal rat hippocampal slice cultures we found that oxygen glucose deprivation (OGD decreased the levels of free carnitines (FC and increased the acylcarnitine (AC: FC ratio. These changes in carnitine homeostasis correlated with decreases in the protein levels of carnitine palmitoyl transferase (CPT 1 and 2. LCAR supplementation prevented the decrease in CPT1 and CPT2, enhanced both FC and the AC∶FC ratio and increased slice culture metabolic viability, the mitochondrial membrane potential prior to OGD and prevented the subsequent loss of neurons during later stages of reperfusion through a reduction in apoptotic cell death. Finally, we found that LCAR supplementation preserved the structural integrity and synaptic transmission within the hippocampus after OGD. Thus, we conclude that LCAR supplementation preserves the key enzymes responsible for maintaining carnitine homeostasis and preserves both cell viability and synaptic transmission after OGD.

  2. Hot topic: Bovine milk samples yielding negative or nonspecific results in bacterial culturing--the possible role of PCR-single strand conformation polymorphism in mastitis diagnosis.

    Science.gov (United States)

    Schwaiger, K; Wimmer, M; Huber-Schlenstedt, R; Fehlings, K; Hölzel, C S; Bauer, J

    2012-01-01

    A large proportion of mastitis milk samples yield negative or nonspecific results (i.e., no mastitis pathogen can be identified) in bacterial culturing. Therefore, the culture-independent PCR-single strand conformation polymorphism method was applied to the investigation of bovine mastitis milk samples. In addition to the known mastitis pathogens, the method was suitable for the detection of fastidious bacteria such as Mycoplasma spp., which are often missed by conventional culturing methods. The detection of Helcococcus ovis in 4 samples might indicate an involvement of this species in pathogenesis of bovine mastitis. In conclusion, PCR-single-strand conformation polymorphism is a promising tool for gaining new insights into the bacteriological etiology of mastitis.

  3. Healing of the canoe: preliminary results of a culturally tailored intervention to prevent substance abuse and promote tribal identity for Native youth in two Pacific Northwest tribes.

    Science.gov (United States)

    Donovan, Dennis M; Thomas, Lisa Rey; Sigo, Robin Little Wing; Price, Laura; Lonczak, Heather; Lawrence, Nigel; Ahvakana, Katie; Austin, Lisette; Lawrence, Albie; Price, Joseph; Purser, Abby; Bagley, Lenora

    2015-01-01

    Using Community-based and Tribal Participatory Research (CBPR/TPR) approaches, an academic-tribal partnership between the University of Washington Alcohol and Drug Abuse Institute and the Suquamish and Port Gamble S'Klallam Tribes developed a culturally grounded social skills intervention to promote increased cultural belonging and prevent substance abuse among tribal youth. Participation in the intervention, which used the Canoe Journey as a metaphor for life, was associated with increased hope, optimism, and self-efficacy and with reduced substance use, as well as with higher levels of cultural identity and knowledge about alcohol and drugs among high school-age tribal youth. These results provide preliminary support for the intervention curricula in promoting positive youth development, an optimistic future orientation, and the reduction of substance use among Native youth.

  4. Showing Value (Editorial

    Directory of Open Access Journals (Sweden)

    Denise Koufogiannakis

    2009-06-01

    librarians on student achievement. Todd notes, “If we do not show value, we will not have a future. Evidence-based practice is not about the survival of school librarians, it’s about the survival of our students” (40. In this issue we feature school libraries and their connection to evidence based practice. Former Editor-in-Chief, Lindsay Glynn, began putting the wheels in motion for this feature almost a year ago. She invited Carol Gordon and Ross Todd to act as guest editors of the section, drawing upon their contacts and previous work in this field. The result is an issue with five feature articles exploring different aspects of the connection between school libraries and evidence based practice, from the theoretical to the practical. In addition, there is a thought-provoking Commentary by David Loertscher, asking whether we need the evolutionary model of evidence based practice, or something more revolutionary!In addition to the Feature section, we have a well-rounded issue with articles on the topics of library human resources, and the development of a scholars’ portal. As well, there are a record 10 evidence summaries and our educational EBL101 column. I hope there is something for everyone in this issue of EBLIP – enjoy, and see you soon in Stockholm!

  5. Throat Culture

    Science.gov (United States)

    ... products and services. Advertising & Sponsorship: Policy | Opportunities Throat Culture Share this page: Was this page helpful? Collecting | ... treatment | Getting results | see BLOOD SAMPLE Collecting A culture is a test that is often used to ...

  6. Preliminary results of implantation in animal model and osteoblast culture evaluation of prototypes of biomimetic multispiked connecting scaffold for noncemented stemless resurfacing hip arthroplasty endoprostheses.

    Science.gov (United States)

    Uklejewski, Ryszard; Rogala, Piotr; Winiecki, Mariusz; Kędzia, Andrzej; Ruszkowski, Piotr

    2013-01-01

    We present the new fixation method for RHA (resurfacing hip arthroplasty) endoprostheses by means of the biomimetic multispiked connecting scaffold (MSC-Scaffold). Such connecting scaffold can generate new type of RHA endoprostheses, that is stemless and fixed entirely without cement. The preprototypes of this MSC-Scaffold were manufactured with modern additive laser additive technology (SLM). The pilot surgical implantations in animal model (two laboratory swine) of MSC-Scaffold preprototypes have showed after two months neither implant loosening, migration, and nor other early complications. From the results of performed histopathological evaluation of the periscaffold spikes bone tissue and 10-day culture of human osteoblasts (NHOst) we can conclude that (1) the scaffolding effect was obtained and (2) to improve the osseointegration of the scaffold spikes, their material surface should be physicochemically modified (e.g., with hydroxyapatite). Some histopathological findings in the periscaffold domain near the MSC-Scaffold spikes bases (fibrous connective tissue and metallic particles near the MSC-Scaffold spikes bases edges) prompt considering the necessity to optimize the design of the MSC-Scaffold in the regions of its interspike space near the spikes bases edges, to provide more room for new bone formation in this region and for indispensable post-processing (glass pearl blasting) after the SLM manufacturing.

  7. Interspecies interactions result in enhanced biofilm formation by co-cultures of bacteria isolated from a food processing environment

    DEFF Research Database (Denmark)

    Røder, Henriette Lyng; Raghupathi, Prem Krishnan; Herschend, Jakob

    2015-01-01

    examined for multispecies biofilm formation. Eight strains from each sampling site were chosen and all possible combinations of four member co-cultures were tested for enhanced biofilm formation at 15°C and 24°C. In approximately 20% of the multispecies consortia grown at 15°C, the biofilm formation......Bacterial attachment and biofilm formation can lead to poor hygienic conditions in food processing environments. Furthermore, interactions between different bacteria may induce or promote biofilm formation. In this study, we isolated and identified a total of 687 bacterial strains from seven...... different locations in a meat processing environment and evaluated their biofilm formation capability. A diverse group of bacteria was isolated and most were classified as poor biofilm producers in a Calgary biofilm device assay. Isolates from two sampling sites, the wall and the meat chopper, were further...

  8. The People's Show: A Critical Analysis

    OpenAIRE

    Robin Francis

    1996-01-01

    The 1990s heralded a new form of museum exhibition: "The People's Show." A light-hearted celebration of popular culture, the concept has had phenomenal success throughout the United Kingdom. Beneath the humour, however, are more complex and radical agendas relating to cultural rights. The paper explores the issues associated with the rise and possible wane of this museum-based popular cultural phenomenon.

  9. O show de Darwin

    Directory of Open Access Journals (Sweden)

    Steve Shapin

    2010-07-01

    Full Text Available As comemorações dos 200 anos de nascimento de Charles Darwin revelaram menos sobre a figura histórica do cientista vitoriano do que sobre o lugar da ciência e do cientista na cultura moderna. O artigo explora os eventos do "Dia de Darwin" e a produção recente sobre o "pai da teoria da evolução" com a intenção de ligá-los à recepção contemporânea da obra (e da figura de Charles Darwin.The celebrations of the 200th anniversary of Charles Darwin's birth were less about the historical figure of the Victorian scientist than about the place of science and the scientist in modern culture. The article explores the "Darwin Day" events and recent books on the "father of the theory of evolution" in order to relate them to the contemporary reception of Darwin's life and work.

  10. Incidence of Salmonella Enteritidis in chicken layer flocks in Turkey: results by real-time polymerase chain reaction and International Organization for Standardization culture methods.

    Science.gov (United States)

    Temelli, S; Kahya, S; Eyigor, A; Carli, K T

    2010-07-01

    This study presents Salmonella Enteritidis incidence in chicken layer flocks in Turkey determined by real-time PCR (rPCR) and by International Organization for Standardization (ISO) method 6579:2002/Amd 1:2007. A total of 259 samples, composed of 1,036 individual samples each pooled into 4, including 175 cloacal swab, 14 intestine, 35 gizzard swab, and 35 cecal swab samples, belonging to 6 major companies, were collected from 50 layer flocks and tested by rPCR and ISO culture methods. Overall incidence of Salmonella in layer flocks by rPCR and culture was 61.0 and 55.6%, respectively, where 70.1% of these Salmonella isolates were determined as Salmonella Enteritidis. Incidences of Salmonella Enteritidis in culture-positive samples were 65.3% in cloacal swabs, 50.0% in intestines, 73.9% in gizzard swabs, and 87.5% in cecal swabs. The rPCR results were in 100% agreement (100% sensitivity and specificity) with culture results when cecal swabs were selected as the sample type. The relative accuracy of rPCR was 92.4, 91.4, and 84% for intestine, gizzard, and cloacal swab samples, respectively. As a result, by using rPCR and ISO culture, we determined that the Salmonella Enteritidis incidence in layer flocks in Turkey was high and that the use of cecal swab and intestine samples in Salmonella detection would yield reliable results. To reduce this high Salmonella Enteritidis incidence in layer flocks, Salmonella Enteritidis-specific vaccination should be implemented properly in conjunction with a well-designed biosecurity plan, including verifiable corrective actions.

  11. The Health Show

    OpenAIRE

    Swann, David

    2011-01-01

    Dr David Swann interviewed on The Health Show, Series 1, Episode 5, 2011 for BBC World about the award-winning 21st Century Nursing Bag. BBC World News reaches 241million people every week, available in 296 million homes, 1.8 million hotel rooms and has the highest average viewership on a weekday of any international news channel. The Health Show is a new 26-part series for BBC World News covering the most important news stories from around the world.

  12. Cultural Rights and Cultural Diversity

    Institute of Scientific and Technical Information of China (English)

    WANG SIXIN

    2011-01-01

    @@ Culture is a very big concept, big enough almost to comprise all the activities of human beings and the tangible and intangible results caused by human activities.Therefore, it is very difficult to define culture in a few words.

  13. A Fashion Show

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    <正>Story: The yearly fashion show day.The children take turns to walk on the stage and show the class their favorite clothes.Now it’s Joe’s and Phoebe’s turn.Joe walks on the stage and says,“My shorts are blue.Do you like my blue shorts?”On the other side of the stage, Phoebe is wearing her favorite pink skirt.“My skirt is pink.Do you like my pink skirt?”asks

  14. On not showing scalps

    DEFF Research Database (Denmark)

    Marselis, Randi Lorenz

    2016-01-01

    proposed by Janet Marstine, the editor of the Routledge Companion to Museum Ethics, I show how the museum succeeded in engaging users in questions of museum ethics. However, this specific debate on human remains in museums developed into an encounter between a global, museological discourse...

  15. Honored Teacher Shows Commitment.

    Science.gov (United States)

    Ratte, Kathy

    1987-01-01

    Part of the acceptance speech of the 1985 National Council for the Social Studies Teacher of the Year, this article describes the censorship experience of this honored social studies teacher. The incident involved the showing of a videotape version of the feature film entitled "The Seduction of Joe Tynan." (JDH)

  16. A Visionary Show

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Seduction. Distinction. Relax. Pulsation. These are the "style universes" on display at Première Vision, heralded as "The World’s Premiere Fabric Show." Started more than 35 years ago by 15 French weavers, Première Vision has expanded beyond its

  17. Honored Teacher Shows Commitment.

    Science.gov (United States)

    Ratte, Kathy

    1987-01-01

    Part of the acceptance speech of the 1985 National Council for the Social Studies Teacher of the Year, this article describes the censorship experience of this honored social studies teacher. The incident involved the showing of a videotape version of the feature film entitled "The Seduction of Joe Tynan." (JDH)

  18. Shanghai Shows Its Heart

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The city known as China’s economic powerhouse showed a more caring face as host of the Special Olympic Games Between October 2 and 11,the Special Olympics Summer Games were hosted in Shanghai,the first time the 40-year-old athletic com- petition for people with intellectual disabilities came to a developing country. This Special Olympics was also larger than all previous games in temps of the number of athletes.

  19. Dietary fiber showed no preventive effect against colon and rectal cancers in Japanese with low fat intake: an analysis from the results of nutrition surveys from 23 Japanese prefectures

    Directory of Open Access Journals (Sweden)

    Sugawara Kazuo

    2001-10-01

    Full Text Available Abstract Background Since Fuchs' report in 1999, the reported protective effect of dietary fiber from colorectal carcinogenesis has led many researchers to question its real benefit. The aim of this study is to evaluate the association between diet, especially dietary fiber and fat and colorectal cancer in Japan. Methods A multiple regression analysis (using the stepwise variable selection method was performed using the standardized mortality ratios (SMRs of colon and rectal cancer in 23 Japanese prefectures as objective variables and dietary fiber, nutrients and food groups as explanatory variables. Results As for colon cancer, the standardized partial correlation coefficients were positively significant for fat (1,13, P = 0.000, seaweeds (0.41, P = 0.026 and beans (0.45, P = 0.017 and were negatively significant for vitamin A (-0.63, P = 0.003, vitamin C (-0.42, P = 0.019 and yellow-green vegetables (-0.37, P = 0.046. For rectal cancer, the standardized partial correlation coefficient in fat (0.60, P = 0.002 was positively significant. Dietary fiber was not found to have a significant relationship with either colon or rectal cancers. Conclusions This study failed to show any protective effect of dietary fiber in subjects with a low fat intake (Japanese in this analysis, which supports Fuchs' findings in subjects with a high fat intake (US Americans.

  20. Instilling a Culture of Accountability at the Department of Homeland Security (DHS): Leveraging the Government Performance and Results Modernization Act (GPRA-MA) of 2010

    Science.gov (United States)

    2013-09-01

    He presages major cultural changes, even a seismic shift in mindset pertaining to government performance and results that will be much more far...holistic and seismic shift within both of these arenas.54 Their model nicely summarizes the bi-furcated philosophical approach to leadership and...responsibility to change, offering a few “government-proof” (i.e., safe) techniques for change. Much like performing acrobatics in a straight jacket , almost

  1. Clinical Neisseria gonorrhoeae isolate with a N. meningitidis porA gene and no prolyliminopeptidase activity, Sweden, 2011: danger of false-negative genetic and culture diagnostic results.

    Science.gov (United States)

    Golparian, D; Johansson, E; Unemo, M

    2012-03-01

    We describe a Neisseria gonorrhoeae strain, found in Sweden in 2011, that harbours a N. meningitidis porA gene causing false-negative results in PCRs targeting the gonococcal porA pseudogene. Furthermore, the strain had no prolyliminopeptidase (PIP) activity that many commercial biochemical kits for species verification in culture rely on. Enhanced awareness of the spread of such strains and screening for them can be crucial.

  2. Seeking Opportunities at The Show

    Institute of Scientific and Technical Information of China (English)

    Zhao Fei

    2010-01-01

    @@ There are four numbers that can describe a city,7300 years of ancestor culture,4300 years of city culture,2700 years the canal culture,and 130 years of industrial culture.Another set of four numbers are: as the Chinese Knitting Culture T-shirt Industry Base,Shizhong district has more than 300 textile clothing enterprises and 50,000 staff; annual production of each kind of clothing is 300 million,and Culture T-shirt is 500 million.The city is Zaozhuang,Shandong province.

  3. Not a "reality" show.

    Science.gov (United States)

    Wrong, Terence; Baumgart, Erica

    2013-01-01

    The authors of the preceding articles raise legitimate questions about patient and staff rights and the unintended consequences of allowing ABC News to film inside teaching hospitals. We explain why we regard their fears as baseless and not supported by what we heard from individuals portrayed in the filming, our decade-long experience making medical documentaries, and the full un-aired context of the scenes shown in the broadcast. The authors don't and can't know what conversations we had, what documents we reviewed, and what protections we put in place in each televised scene. Finally, we hope to correct several misleading examples cited by the authors as well as their offhand mischaracterization of our program as a "reality" show.

  4. 1 341 cases of blood culture results and antibiotic resistance analysis%1341例血培养结果和药敏分析

    Institute of Scientific and Technical Information of China (English)

    丁耀菁

    2011-01-01

    Objective: To investigate the distribution of blood germ culture and its drug fast condition.Methods: Isolating bacteria in 1 341 hospitalized patients with blood culture specimen in our hospital from January 2008 to December 2010 and its drug fast condition were analyzed retrospectively.Results: The result of blood culture indicated that Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis were the major pathogenic bacteria, Gram negative bacillus had 100% sensitivity to imipenem and meropenem.Vancomycin are sensitive to gram positive coccus.Conclusion: Blood culture and drug sensitivity tencency, it will provide science evidences for the rational use of antibiotics.%目的:了解血培养常见病原菌及其药敏情况.方法:对我院2008年1月~2010年12月住院患者1 341例血培养结果进行病原学和药敏情况分析.结果:培养结果揭示病原菌以大肠埃希菌、肺炎克雷伯菌、金黄色葡萄球菌、表皮葡萄球菌为本研究病例中主要病原菌.革兰阴性杆菌对亚胺培南、美罗培南敏感率为100%,革兰阳性球菌对万古霉素敏感.结论:血培养和药敏结果可为临床诊治血液感染提供科学依据.

  5. The Cultural Politics of National Testing and Test Result Release Policy in South Korea: A Critical Discourse Analysis

    Science.gov (United States)

    Sung, Youl-Kwan; Kang, Mi Ok

    2012-01-01

    This paper examines the ideological construction of educational discourses embedded within the South Korean print media. Significantly, these discourses have recently promoted the resurrection of a sweeping national testing and test results release policy. Through careful examination of the "test plus release" policy, the authors show…

  6. Gene disruption of Plasmodium falciparum p52 results in attenuation of malaria liver stage development in cultured primary human hepatocytes.

    Directory of Open Access Journals (Sweden)

    Ben C L van Schaijk

    Full Text Available Difficulties with inducing sterile and long lasting protective immunity against malaria with subunit vaccines has renewed interest in vaccinations with attenuated Plasmodium parasites. Immunizations with sporozoites that are attenuated by radiation (RAS can induce strong protective immunity both in humans and rodent models of malaria. Recently, in rodent parasites it has been shown that through the deletion of a single gene, sporozoites can also become attenuated in liver stage development and, importantly, immunization with these sporozoites results in immune responses identical to RAS. The promise of vaccination using these genetically attenuated sporozoites (GAS depends on translating the results in rodent malaria models to human malaria. In this study, we perform the first essential step in this transition by disrupting, p52, in P. falciparum an ortholog of the rodent parasite gene, p36p, which we had previously shown can confer long lasting protective immunity in mice. These P. falciparum P52 deficient sporozoites demonstrate gliding motility, cell traversal and an invasion rate into primary human hepatocytes in vitro that is comparable to wild type sporozoites. However, inside the host hepatocyte development is arrested very soon after invasion. This study reveals, for the first time, that disrupting the equivalent gene in both P. falciparum and rodent malaria Plasmodium species generates parasites that become similarly arrested during liver stage development and these results pave the way for further development of GAS for human use.

  7. Myo1e impairment results in actin reorganization, podocyte dysfunction, and proteinuria in zebrafish and cultured podocytes.

    Directory of Open Access Journals (Sweden)

    Jianhua Mao

    Full Text Available BACKGROUND: Podocytes serve as an important constituent of the glomerular filtration barrier. Recently, we and others identified Myo1e as a key molecular component of the podocyte cytoskeleton. RESULTS: Myo1e mRNA and protein was expressed in human and mouse kidney sections as determined by Northern blot and reverse transcriptase PCR, and its expression was more evident in podocytes by immunofluorescence. By specific knock-down of MYO1E in zebrafish, the injected larvae exhibited pericardial edema and pronephric cysts, consistent with the appearance of protein in condensed incubation supernate. Furthermore, specific inhibition of Myo1e expression in a conditionally immortalized podocyte cell line induced morphological changes, actin cytoskeleton rearrangement, and dysfunction in cell proliferation, migration, endocytosis, and adhesion with the glomerular basement membrane. CONCLUSIONS: Our results revealed that Myo1e is a key component contributing to the functional integrity of podocytes. Its impairment may cause actin cytoskeleton re-organization, alteration of cell shape, and membrane transport, and podocyte drop-out from the glomerular basement membrane, which might eventually lead to an impaired glomerular filtration barrier and proteinuria.

  8. Simvastatin Results in a Dose-Dependent Toxic Effect on Spiral Ganglion Neurons in an In Vitro Organotypic Culture Assay

    Directory of Open Access Journals (Sweden)

    Katharina Leitmeyer

    2016-01-01

    Full Text Available Statins are inhibitors of the 3-hydroxy-3-methylglutaryl-coenzyme A reductase, an enzyme necessary for the production of mevalonate. They are widely used as cholesterol-lowering drugs. However, conflicting data about the effect of statins on neuronal cells has been published. To explore the effect of simvastatin on spiral ganglion neurons (SGNs, SG explants of 5-day-old rats were treated with increasing concentrations of simvastatin. In addition, SG explants were treated with mevalonate and with the combination of simvastatin and mevalonate. SGN number, length of the neurites, area of nonneuronal supporting cells, and neuronal survival were analyzed. Simvastatin treatment results in a significant dose-dependent decrease of SG neurite number, length of neurites, area of supporting cells, and SG neuronal survival compared to control. Interestingly, treatment with mevalonate in addition to simvastatin increased SG neuronal survival compared to simvastatin treatment only. However, treatment with mevalonate in addition to simvastatin did not influence SG neurite number, length of neurites, and area of supporting cells compared to simvastatin treatment only. Our results suggest a neurotoxic effect of simvastatin on SGNs in vitro. Neurotoxicity seems to be at least partially mediated by the mevalonate pathway. Therefore, caution is warranted to use simvastatin as a potential otoprotective drug.

  9. Children’s School-Breakfast Reports and School-Lunch Reports (in 24-hour Dietary Recalls): Conventional and Reporting-Error-Sensitive Measures Show Inconsistent Accuracy Results for Retention Interval and for Breakfast Location

    Science.gov (United States)

    Baxter, Suzanne Domel; Guinn, Caroline H.; Smith, Albert F.; Hitchcock, David B.; Royer, Julie A.; Puryear, Megan P.; Collins, Kathleen L.; Smith, Alyssa L.

    2017-01-01

    Validation-study data were analyzed to investigate retention interval (RI) and prompt effects on accuracy of fourth-grade children’s reports of school-breakfast and school-lunch (in 24-hour recalls), and accuracy of school-breakfast reports by breakfast location (classroom; cafeteria). Randomly-selected fourth-grade children at 10 schools in four districts were observed eating school-provided breakfast and lunch, and interviewed under one of eight conditions (two RIs [short (prior-24-hour recall obtained in afternoon); long (previous-day recall obtained in morning)] crossed with four prompts [forward (distant-to-recent), meal-name (breakfast, etc.), open (no instructions), reverse (recent-to-distant)]). Each condition had 60 children (half girls). Of 480 children, 355 and 409 reported meals satisfying criteria for reports of school-breakfast and school-lunch, respectively. For breakfast and lunch separately, a conventional measure—report rate—and reporting-error-sensitive measures—correspondence rate and inflation ratio—were calculated for energy per meal-reporting child. Correspondence rate and inflation ratio—but not report rate—showed better accuracy for school-breakfast and school-lunch reports with the short than long RI; this pattern was not found for some prompts for each sex. Correspondence rate and inflation ratio showed better school-breakfast report accuracy for the classroom than cafeteria location for each prompt, but report rate showed the opposite. For each RI, correspondence rate and inflation ratio showed better accuracy for lunch than breakfast, but report rate showed the opposite. When choosing RI and prompts for recalls, researchers and practitioners should select short RIs to maximize accuracy. Recommendations for prompt selections are less clear. As report rates distort validation-study accuracy conclusions, reporting-error-sensitive measures are recommended. PMID:26865356

  10. Evaluation of the association between Hospital Survey on Patient Safety Culture (HSOPS) measures and catheter-associated infections: results of two national collaboratives

    Science.gov (United States)

    Meddings, Jennifer; Reichert, Heidi; Greene, M Todd; Safdar, Nasia; Krein, Sarah L; Olmsted, Russell N; Watson, Sam R; Edson, Barbara; Albert Lesher, Mariana; Saint, Sanjay

    2017-01-01

    Background The Agency for Healthcare Research and Quality (AHRQ) has funded national collaboratives using the Comprehensive Unit-based Safety Program to reduce rates of two catheter-associated infections—central-line-associated bloodstream infection (CLABSI) and catheter-associated urinary tract infection (CAUTI), using evidence-based intervention bundles to improve technical aspects of care and socioadaptive approaches to foster a culture of safety. Objective Examine the association between hospital units' results for the Hospital Survey on Patient Safety Culture (HSOPS) and catheter-associated infection rates. Methods We analysed data from two prospective cohort studies from acute-care intensive care units (ICUs) and non-ICUs participating in the AHRQ CLABSI and CAUTI collaboratives. National Healthcare Safety Network catheter-associated infections per 1000 catheter-days were collected at baseline and quarterly postimplementation. The HSOPS was collected at baseline and again 1 year later. Infection rates were modelled using multilevel negative binomial models as a function of HSOPS components over time, adjusted for hospital-level characteristics. Results 1821 units from 1079 hospitals (CLABSI) and 1576 units from 949 hospitals (CAUTI) were included. Among responding units, infection rates declined over the project periods (by 47% for CLABSI, by 23% for CAUTI, unadjusted). No significant associations were found between CLABSI or CAUTI rates and HSOPS measures at baseline or over time. Conclusions We found no association between results of the HSOPS and catheter-associated infection rates when measured at baseline and postintervention in two successful large national collaboratives focused on prevention of CLABSI and CAUTI. These results suggest that it may be possible to improve CLABSI and CAUTI rates without making significant changes in safety culture, particularly as measured by instruments like HSOPS. PMID:27222593

  11. In favour of the definition "adolescents with idiopathic scoliosis": juvenile and adolescent idiopathic scoliosis braced after ten years of age, do not show different end results. SOSORT award winner 2014

    OpenAIRE

    Donzelli, Sabrina; Zaina, Fabio; Lusini, Monia; Minnella, Salvatore; Negrini, Stefano

    2014-01-01

    Background The most important factor discriminating juvenile (JIS) from adolescent idiopathic scoliosis (AIS) is the risk of deformity progression. Brace treatment can change natural history, even when risk of progression is high. The aim of this study was to compare the end of growth results of JIS subjects, treated after 10 years of age, with final results of AIS. Methods Design: prospective observational controlled cohort study nested in a prospective database. Setting: outpatient tertiary...

  12. Correlation between Clostridium difficile bacterial load, commercial real-time PCR cycle thresholds, and results of diagnostic tests based on enzyme immunoassay and cell culture cytotoxicity assay.

    Science.gov (United States)

    Dionne, Léa-Laurence; Raymond, Frédéric; Corbeil, Jacques; Longtin, Jean; Gervais, Philippe; Longtin, Yves

    2013-11-01

    The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four assays: a glutamate dehydrogenase (GDH) enzyme immunoassay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture cytotoxicity assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold (CT) with the results of quantitative culture using Spearman's rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR(+) (group 1), 37 PCR(+) GDH(+) (group 2), 24 PCR(+) GDH(+) CCA(+) (group 3), and 125 PCR(+) GDH(+) ToxAB(+) (group 4). The overall median fecal load in log10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups (P tests.

  13. Analysis of positive result of blood culture with BACTEC FX automatic blood culture system and its application%BACTEC FX 全自动血培养仪阳性结果分析及应用评价

    Institute of Scientific and Technical Information of China (English)

    麦珍; 朱雄; 陈海; 李欢; 黎元莉; 黎礼达

    2014-01-01

    目的:对美国BD公司 BACTEC FX全自动血培养仪进行血培养结果分析,并对其临床应用进行评价。方法收集2011年10月-2012年11月医院收治怀疑血流感染患者的血液标本,血培养及鉴定采用美国BD公司BACTEC FX全自动血培养仪和BD Phoenix l00自动细菌鉴定仪。结果2590份血液标本中实际检出阳性标本264份,阳性率10.2%,假阳性率0.15%;264株病原菌中,最快检出时间为2.7 h ,有26.9%在12 h内检出,51.1%在24 h内检出,86.0%在48 h内检出,95.8%在72 h内检出;有40.5%标本厌氧瓶先报警、需氧瓶后报警,34.5%标本需氧瓶先报警、厌氧瓶后报警,11.8%标本需氧瓶先报警、但厌氧瓶不报警。结论美国BD公司BACTEC FX全自动血培养仪操作简便、灵敏度高,提高了细菌检出的阳性率及种类,检出速度更快捷、更准确,为治疗患者赢得了宝贵的时间。%OBJECTIVE To analyze the result of blood culture with BACTEC FX automatic blood culture system of BD company of America and evaluate the clinical application .METHODS The blood specimens were collected from the patients with suspected bloodstream infections who were treated in the hospital from Oct 2011 to Nov 2012 , then the blood culture and the identification of bacteria were performed with the use of BACTEC FX automatic blood culture system of BD company of America and the BD Phoenix l00 automatic bacteria identification system . RESULTS Of the 2 590 blood specimens ,264 were actually cultured positive with the positive rate of 10 .2% and the false positive rate of 0 .15% .Among the 264 strains of isolated pathogens ,the rapidest detection time was 2 .7 hours ,26 .9% of the pathogens were detected within 12 hours ,51 .1% detected within 24 hours ,86 .0% detected within 48 hours ,95 .8% detected within 72 hours .40 .5% of the specimens firstly alarmed in the anaerobic bottles then in the aerobic bottles

  14. SYMBOLICS IN NATIONAL CULTURE

    Directory of Open Access Journals (Sweden)

    G. M. Gadzhinayev

    2013-01-01

    Full Text Available Abstract. Features of national culture as component of culture of ethnos, and as phenomenon of social and historical and cultural life of the people are defined in the article.National artist of force of space felt in all that was for it considerable in nature and in creations of a man. Human it is learned through natural, natural through the human. Animals, birds, plants personify forces of nature, serve as the symbols connecting people with space.It was manysets of various symbols, magic signs in the dwelling andhouse furniture, which subsequently some have lost initial magic value, having given way, mainly, to esthetic appointment. Ornaments of embroideries and jewelry, ornamental compositions and elements of symbols of other nationalities show it. Every ensemble of art have the semantic loading designating the age characteristic and a social standing.Results of the work can be used as information for the tourist organizations, in esthetics, cultural science, culture ecology.

  15. Social learning in a high-risk environment: incomplete disregard for the 'minnow that cried pike' results in culturally transmitted neophobia.

    Science.gov (United States)

    Crane, Adam L; Mathiron, Anthony G E; Ferrari, Maud C O

    2015-08-07

    Many prey species rely on conspecifics to gather information about unknown predation threats, but little is known about the role of varying environmental conditions on the efficacy of social learning. We examined predator-naive minnows that had the opportunity to learn about predators from experienced models that were raised in either a low- or high-risk environment. There were striking differences in behaviour among models; high-risk models showed a weaker response to the predator cue and became neophobic in response to the control cue (a novel odour, NO). Observers that were previously paired with low-risk models acquired a strong antipredator response only to the predator cue. However, observers that interacted with high-risk models, displayed a much weaker response to the predator odour and a weak neophobic response to the NO. This is the first study reporting such different outcomes of social learning under different environmental conditions, and suggests high-risk environments promote the cultural transmission of neophobia more so than social learning. If such a transfer can be considered similar to secondary traumatization in humans, culturally transmitted neophobia in minnows may provide a good model system for understanding more about the social ecology of fear disorders. © 2015 The Author(s).

  16. Peptidomic profiling of secreted products from pancreatic islet culture results in a higher yield of full-length peptide hormones than found using cell lysis procedures.

    Science.gov (United States)

    Taylor, Steven W; Nikoulina, Svetlana E; Andon, Nancy L; Lowe, Carolyn

    2013-08-02

    Peptide Hormone Acquisition through Smart Sampling Technique-Mass Spectrometry (PHASST-MS) is a peptidomics platform that employs high resolution liquid chromatography-mass spectrometry (LC-MS) techniques to identify peptide hormones secreted from in vitro or ex vivo cultures enriched in endocrine cells. Application of the methodology to the study of murine pancreatic islets has permitted evaluation of the strengths and weaknesses of the approach, as well as comparison of our results with published islet studies that employed traditional cellular lysis procedures. We found that, while our PHASST-MS approach identified fewer peptides in total, we had greater representation of intact peptide hormones. The technique was further refined to improve coverage of hydrophilic as well as hydrophobic peptides and subsequently applied to human pancreatic islet cultures derived from normal donors or donors with type 2 diabetes. Interestingly, in addition to the expected islet hormones, we identified alpha-cell-derived bioactive GLP-1, consistent with recent reports of paracrine effects of this hormone on beta-cell function. We also identified many novel peptides derived from neurohormonal precursors and proteins related to the cell secretory system. Taken together, these results suggest the PHASST-MS strategy of focusing on cellular secreted products rather than the total tissue peptidome may improve the probability of discovering novel bioactive peptides and also has the potential to offer important new insights into the secretion and function of known hormones.

  17. Unity in diversity: results of a randomized clinical culturally tailored pilot HIV prevention intervention trial in Baltimore, Maryland, for African American men who have sex with men.

    Science.gov (United States)

    Tobin, Karin; Kuramoto, Satoko J; German, Danielle; Fields, Errol; Spikes, Pilgrim S; Patterson, Jocelyn; Latkin, Carl

    2013-06-01

    Unity in Diversity was a randomized controlled trial of a culturally tailored HIV prevention intervention for African American men who have sex with men. The intervention condition was six group-based sessions and one individual session. The control condition was a single-session HIV prevention review. Participants were aged 18 years or older, identified as African American/Black race, reported having at least two sex partners in the prior 90 days (at least one of whom must be a male partner), unprotected anal sex with male partner in the prior 90 days, and willing to test for HIV. Retention exceeded 95% at 3-month follow-up. Results of multivariate logistic regression analysis adjusting for baseline risk, HIV status, and health insurance indicate intervention efficacy in decreasing the number of male sex partners and marginal effects on condom use with male partners and HIV-negative/unknown partners. Specifically, intervention condition was associated with increased odds of zero male sex partners (adjusted odds ratio [AOR] = 3.03, 95% confidence interval [CI] = 1.26-7.28), condom use with male partners (AOR = 2.64, 95% CI = 0.95-7.36), and HIV-negative/unknown status partners (AOR = 3.19, 95% CI = 0.98-10.38) at follow-up. These results contribute to the limited number of culturally appropriate models of HIV prevention intervention that are urgently needed for African American men who have sex with men to address their persistently high rates of HIV.

  18. Results of a psychosomatic training program in China, Vietnam and Laos: successful cross-cultural transfer of a postgraduate training program for medical doctors

    Directory of Open Access Journals (Sweden)

    Fritzsche Kurt

    2012-08-01

    Full Text Available Abstract Background With the “ASIA-LINK” program, the European Community has supported the development and implementation of a curriculum of postgraduate psychosomatic training for medical doctors in China, Vietnam and Laos. Currently, these three countries are undergoing great social, economic and cultural changes. The associated psychosocial stress has led to increases in psychological and psychosomatic problems, as well as disorders for which no adequate medical or psychological care is available, even in cities. Health care in these three countries is characterized by the coexistence of Western medicine and traditional medicine. Psychological and psychosomatic disorders and problems are insufficiently recognized and treated, and there is a need for biopsychosocially orientated medical care. Little is known about the transferability of Western-oriented psychosomatic training programs in the Southeast Asian cultural context. Methods The curriculum was developed and implemented in three steps: 1 an experimental phase to build a future teacher group; 2 a joint training program for future teachers and German teachers; and 3 training by Asian trainers that was supervised by German teachers. The didactic elements included live patient interviews, lectures, communication skills training and Balint groups. The training was evaluated using questionnaires for the participants and interviews of the German teachers and the future teachers. Results Regional training centers were formed in China (Shanghai, Vietnam (Ho Chi Minh City and Hue and Laos (Vientiane. A total of 200 physicians completed the training, and 30 physicians acquired the status of future teacher. The acceptance of the training was high, and feelings of competence increased during the courses. The interactive training methods were greatly appreciated, with the skills training and self-experience ranked as the most important topics. Adaptations to the cultural background of the

  19. The "Life Potential": a new complex algorithm to assess "Heart Rate Variability" from Holter records for cognitive and diagnostic aims. Preliminary experimental results showing its dependence on age, gender and health conditions

    CERN Document Server

    Barra, Orazio A

    2013-01-01

    Although HRV (Heart Rate Variability) analyses have been carried out for several decades, several limiting factors still make these analyses useless from a clinical point of view. The present paper aims at overcoming some of these limits by introducing the "Life Potential" (BMP), a new mathematical algorithm which seems to exhibit surprising cognitive and predictive capabilities. BMP is defined as a linear combination of five HRV Non-Linear Variables, in turn derived from the thermodynamic formalism of chaotic dynamic systems. The paper presents experimental measurements of BMP (Average Values and Standard Deviations) derived from 1048 Holter tests, matched in age and gender, including a control group of 356 healthy subjects. The main results are: (a) BMP always decreases when the age increases, and its dependence on age and gender is well established; (b) the shape of the age dependence within "healthy people" is different from that found in the general group: this behavior provides evidence of possible illn...

  20. Adaptação cultural e confiabilidade para o Brasil do Automated Telephone Disease Management: resultados preliminares Adaptación cultural y confiabilidad para el Brasil del Automated Telephone Disease Management: resultados preliminares Cultural adaptation and reliability for Brazil of the Automated Telephone Disease Management: Preliminary results

    Directory of Open Access Journals (Sweden)

    Talita Balaminut

    2012-01-01

    study whose cultural adaptation process included: translation, expert committee, back translation, semantic analysis and pretesting. This study included a sample of 39 Brazilian adults with DM enrolled in an educational program in São Paulo. RESULTS: The adapted version of the instrument showed good acceptance with easy comprehension of the items by the participants, with reliability ranging between 0.30 and 0.43. CONCLUSION: After analyzing the psychometric properties and finalizing the validation process in the country, the instrument can be used by Brazilian researchers, making it possible to compare with other cultures.

  1. Evaluation of real-time data obtained from gravimetric preparation of antineoplastic agents shows medication errors with possible critical therapeutic impact: Results of a large-scale, multicentre, multinational, retrospective study.

    Science.gov (United States)

    Terkola, R; Czejka, M; Bérubé, J

    2017-08-01

    Medication errors are a significant cause of morbidity and mortality especially with antineoplastic drugs, owing to their narrow therapeutic index. Gravimetric workflow software systems have the potential to reduce volumetric errors during intravenous antineoplastic drug preparation which may occur when verification is reliant on visual inspection. Our aim was to detect medication errors with possible critical therapeutic impact as determined by the rate of prevented medication errors in chemotherapy compounding after implementation of gravimetric measurement. A large-scale, retrospective analysis of data was carried out, related to medication errors identified during preparation of antineoplastic drugs in 10 pharmacy services ("centres") in five European countries following the introduction of an intravenous workflow software gravimetric system. Errors were defined as errors in dose volumes outside tolerance levels, identified during weighing stages of preparation of chemotherapy solutions which would not otherwise have been detected by conventional visual inspection. The gravimetric system detected that 7.89% of the 759 060 doses of antineoplastic drugs prepared at participating centres between July 2011 and October 2015 had error levels outside the accepted tolerance range set by individual centres, and prevented these doses from reaching patients. The proportion of antineoplastic preparations with deviations >10% ranged from 0.49% to 5.04% across sites, with a mean of 2.25%. The proportion of preparations with deviations >20% ranged from 0.21% to 1.27% across sites, with a mean of 0.71%. There was considerable variation in error levels for different antineoplastic agents. Introduction of a gravimetric preparation system for antineoplastic agents detected and prevented dosing errors which would not have been recognized with traditional methods and could have resulted in toxicity or suboptimal therapeutic outcomes for patients undergoing anticancer treatment.

  2. Short term results of complete (D3) vs. standard (D2) mesenteric excision in colon cancer shows improved outcome of complete mesenteric excision in patients with TNM stages I-II.

    Science.gov (United States)

    Storli, K E; Søndenaa, K; Furnes, B; Nesvik, I; Gudlaugsson, E; Bukholm, I; Eide, G E

    2014-06-01

    The aim of the present study was to investigate whether the new method of complete mesocolic excision (CME) with a high (apical) vascular tie (D3 resection) had an immediate effect compared with a conventional (standard) approach even in those patients without lymph node metastases. A cohort of 189 consecutive patients with tumour-nodal-metastasis (TNM) stages I-II and a mean age of 73 years were operated on in the period from January 2007 to December 2008 in three community teaching hospitals. The CME approach (n = 89), used in hospital A, was compared to the standard technique used (n = 105) in two other hospitals, B and C. Lymph node yields from the specimens were used as a surrogate measure of radical resections. Outcome was analysed after a median follow-up of 50.2 months. In-hospital mortality rate was 2.8 % in the CME group and 8.6 % in the standard group. The 3-year overall survival (OS) in the CME group was 88.1 versus 79.0 % (p = 0.003) in the standard group, and the corresponding disease-free survival (DFS) was 82.1 versus 74.3 % (p = 0.026). Cancer-specific survival was 95.2 % in the CME group versus 90.5 % in the standard group (p = 0.067). Age, operative technique, and T category were significant in multiple Cox regressions of OS and DFS. Compared with the standard (D2) approach, introduction of CME surgical management of colon cancer resulted in a significant immediate improvement of 3-year survival for patients with TNM stage I-II tumours as assessed by OS and DFS.

  3. Novel recurrent chromosomal aberrations detected in clonal plasma cells of light chain amyloidosis patients show potential adverse prognostic effect: first results from a genome-wide copy number array analysis.

    Science.gov (United States)

    Granzow, Martin; Hegenbart, Ute; Hinderhofer, Katrin; Hose, Dirk; Seckinger, Anja; Bochtler, Tilmann; Hemminki, Kari; Goldschmidt, Hartmut; Schönland, Stefan O; Jauch, Anna

    2017-07-01

    Immunoglobulin light chain (AL) amyloidosis is a rare plasma cell dyscrasia characterized by the deposition of abnormal amyloid fibrils in multiple organs, thus impairing their function. In the largest cohort studied up to now of 118 CD138-purified plasma cell samples from previously untreated immunoglobulin light chain amyloidosis patients, we assessed in parallel copy number alterations using high-density copy number arrays and interphase fluorescence in situ hybridization (iFISH). We used fluorescence in situ hybridization probes for the IgH translocations t(11;14), t(4;14), and t(14;16) or any other IgH rearrangement as well as numerical aberrations of the chromosome loci 1q21, 8p21, 5p15/5q35, 11q22.3 or 11q23, 13q14, 15q22, 17p13, and 19q13. Recurrent gains included chromosomes 1q (36%), 9 (24%), 11q (24%), as well as 19 (15%). Recurrent losses affected chromosome 13 (29% monosomy) and partial losses of 14q (19%), 16q (14%) and 13q (12%), respectively. In 88% of patients with translocation t(11;14), the hallmark chromosomal aberration in AL amyloidosis, a concomitant gain of 11q22.3/11q23 detected by iFISH was part of the unbalanced translocation der(14)t(11;14)(q13;q32) with the breakpoint in the CCND1/MYEOV gene region. Partial loss of chromosome regions 14q and 16q were significantly associated to gain 1q. Gain 1q21 detected by iFISH almost always resulted from a gain of the long arm of chromosome 1 and not from trisomy 1, whereas deletions on chromosome 1p were rarely found. Overall and event-free survival analysis found a potential adverse prognostic effect of concomitant gain 1q and deletion 14q as well as of deletion 1p. In conclusion, in the first whole genome report of clonal plasma cells in AL amyloidosis, novel aberrations and hitherto unknown potential adverse prognostic effects were uncovered. Copyright© 2017 Ferrata Storti Foundation.

  4. Factors that Predict Negative Results of QuantiFERON-TB Gold In-Tube Test in Patients with Culture-Confirmed Tuberculosis: A Multicenter Retrospective Cohort Study.

    Directory of Open Access Journals (Sweden)

    Yong-Soo Kwon

    Full Text Available Interferon-γ release assays such as the QuantiFERON-TB Gold In-Tube Test (QFT-GIT are designed to detect Mycobacterium tuberculosis infections, whether latent or manifesting as disease. However, a substantial number of persons with culture-confirmed tuberculosis (TB have negative QFT-GITs. Information on host factors contributing to false-negative and indeterminate results are limited.A multicenter retrospective cohort study was performed with 1,264 culture-confirmed TB patients older than 18 years who were subjected to the QFT-GIT at one of the six hospitals between May 2007 and February 2014. Patients with human immunodeficiency virus infection were excluded. Clinical and laboratory data were collected in South Korea.Of all patients, 87.6% (1,107/1,264 were diagnosed with pulmonary TB and 12.4% (157/1,264 with extrapulmonary TB. The rate of negative results was 14.4% (182/1,264. The following factors were highly correlated with false-negative results in the QFT-GIT: advanced age (age ≥ 65 years, odds ratio [OR] 1.57, 95% confidence interval [CI] 1.03-2.39, bilateral disease as determined by chest radiography (OR 1.75, 95% CI 1.13-2.72, malignancy (OR 2.42, 95% CI 1.30-4.49, and lymphocytopenia (total lymphocyte count < 1.0 × 109/L, OR 1.86, 95% CI 1.21-2.87.Consequently, QFT-GIT results need to be interpreted with caution in patients with these host risk factors such as the elderly, bilateral disease on chest radiography, or malignancy, or lymphocytopenia.

  5. Critical Literacy: Does Advertising Show Gender and Cultural Stereotyping?

    Science.gov (United States)

    Russo, Elizabeth

    1996-01-01

    The critical literacy component of an adult program developed skills in analyzing media advertising; using math for data analysis, graphing, and computation; interpreting data; and becoming aware of advertising's part in reenforcing gender roles. (SK)

  6. Different Cultures Show Same Respect for Human Dignity

    Institute of Scientific and Technical Information of China (English)

    Luo Haocai

    2012-01-01

    In September the clouds are thin and the sky is high in Beijing.In such a fine season,the 4th Beijing Forum on Human Rights,jointly sponsored by the China Society for Human Rights Studies and the China Foundation for Human Rights Development opens today.On behalf of the sponsors,I extend my warm welcome and sincere thanks to all friends who have come to attend this forum.

  7. Enterococcus faecalis strains show culture heterogeneity in cell surface charge

    NARCIS (Netherlands)

    van Merode, Annet; van der Mei, HC; Busscher, HJ; Waar, K; Krom, BP

    2006-01-01

    Adhesion of micro-organisms to biotic and abiotic surfaces is an important virulence factor and involves different types of interactions. Enterococcus faecalis, a human commensal and an important opportunistic pathogen, has the ability to adhere to surfaces. Biliary stents frequently become clogged

  8. Effects of ethanol on voltage-sensitive Na-channels in cultured skeletal muscle: Up-regulation as a result of chronic treatment

    Energy Technology Data Exchange (ETDEWEB)

    Brodie, C.; Sampson, S.R. (Bar-Ilan Univ., Ramat-Gan (Israel))

    1990-12-01

    The effects of acute and chronic treatment with ethanol were studied on the number and activity of tetrodotoxin-sensitive Na-channels in cultured rat skeletal muscle. The number of channels was determined by measurements of specific binding of (3H) saxitoxin (STX) in whole cell preparations. Measurements were also made of the frequency and rate of rise of spontaneously occurring action potentials, which are the physiologic expression of Na-channel density. Acute ethanol (37.5-150 mM), while causing depolarization of membrane potential and blockade of electrical activity, was without effect on specific STX binding. Neither methanol, acetaldehyde nor ethylene glycol had significant effects on these properties when given acutely in the same concentrations as ethanol. Chronic ethanol caused dose-related increases in STX binding and action potential properties with maximal levels being attained after 3 days of treatment at a concentration of 150 mM. On removal of ethanol from the culture medium all properties returned to control levels after 48 hr. Both increased external K+ and tetrodotoxin, which up-regulate Na-channels by reducing cytosolic Ca++, potentiated the ethanol-induced increase in Na-channel density. The increase in STX binding was not associated with changes in affinity of the binding sites for the ligand but was completely prevented by treatment with cycloheximide and actinomycin D. The results demonstrate that ethanol interacts with the cell membrane to induce synthesis of STX-binding sites.

  9. “Spiritual and Moral Culture in the Higher Education” Expectations and fears (the results of ‘Christmas Educational Readings’ in PFUR

    Directory of Open Access Journals (Sweden)

    Zh V Puzanova

    2016-12-01

    Full Text Available The article provides an overview of the key issues of the III International Conference “Spiritual and Moral Culture in the Higher Education” devoted to the discussion of the action plan to implement the National Strategy of Education Development until 2025. The authors consider the basic ideas of the key conference speakers’ presentations and present the results of the express-poll among the conference participants conducted to identify their expectations. The main leitmotif of all speakers’ presentations was as follows: the higher school is responsible for training the new generations of professionals - with high moral standards and responsibility for the fate of the country and one’s homeland. Young Russian citizens focused on fruitful activity can be raised only by joint efforts of the state, the church and society. It is extremely important to coordinate the efforts of the church and the state in the support and protection of the family, in combating extremism, and in forming high moral standards. ‘Christmas Readings’ is a good tradition established several years ago, which continues to bring together people interested in solving the most urgent problems in the educational sphere. The participants of the round tables discussed the issues of youth education and its impact on the country’s national security, the relationships of spiritual values and nation’s health, pedagogical aspects of spiritual and moral education of the youth, the impact of mass culture on the society, the student volunteer movement, spiritual and moral criteria of science.

  10. Stem cells show promising results for lymphoedema treatment

    DEFF Research Database (Denmark)

    Toyserkani, Navid Mohamadpour; Quaade, Marlene Louise; Sheikh, Søren Paludan

    2015-01-01

    Abstract Lymphoedema is a debilitating condition, manifesting in excess lymphatic fluid and swelling of subcutaneous tissues. Lymphoedema is as of yet still an incurable condition and current treatment modalities are not satisfactory. The capacity of mesenchymal stem cells to promote angiogenesis......, secrete growth factors, regulate the inflammatory process, and differentiate into multiple cell types make them a potential ideal therapy for lymphoedema. Adipose tissue is the richest and most accessible source of mesenchymal stem cells and they can be harvested, isolated, and used for therapy...... in a single stage procedure as an autologous treatment. The aim of this paper was to review all studies using mesenchymal stem cells for lymphoedema treatment with a special focus on the potential use of adipose-derived stem cells. A systematic search was performed and five preclinical and two clinical...

  11. Variable cultural acquisition costs constrain cumulative cultural evolution.

    Directory of Open Access Journals (Sweden)

    Alex Mesoudi

    Full Text Available One of the hallmarks of the human species is our capacity for cumulative culture, in which beneficial knowledge and technology is accumulated over successive generations. Yet previous analyses of cumulative cultural change have failed to consider the possibility that as cultural complexity accumulates, it becomes increasingly costly for each new generation to acquire from the previous generation. In principle this may result in an upper limit on the cultural complexity that can be accumulated, at which point accumulated knowledge is so costly and time-consuming to acquire that further innovation is not possible. In this paper I first review existing empirical analyses of the history of science and technology that support the possibility that cultural acquisition costs may constrain cumulative cultural evolution. I then present macroscopic and individual-based models of cumulative cultural evolution that explore the consequences of this assumption of variable cultural acquisition costs, showing that making acquisition costs vary with cultural complexity causes the latter to reach an upper limit above which no further innovation can occur. These models further explore the consequences of different cultural transmission rules (directly biased, indirectly biased and unbiased transmission, population size, and cultural innovations that themselves reduce innovation or acquisition costs.

  12. Culture Negative Listeria monocytogenes Meningitis Resulting in Hydrocephalus and Severe Neurological Sequelae in a Previously Healthy Immunocompetent Man with Penicillin Allergy

    Directory of Open Access Journals (Sweden)

    Shahin Gaini

    2015-01-01

    Full Text Available A previously healthy 74-year-old Caucasian man with penicillin allergy was admitted with evolving headache, confusion, fever, and neck stiffness. Treatment for bacterial meningitis with dexamethasone and monotherapy ceftriaxone was started. The cerebrospinal fluid showed negative microscopy for bacteria, no bacterial growth, and negative polymerase chain reaction for bacterial DNA. The patient developed hydrocephalus on a second CT scan of the brain on the 5th day of admission. An external ventricular catheter was inserted and Listeria monocytogenes grew in the cerebrospinal fluid from the catheter. The patient had severe neurological sequelae. This case report emphasises the importance of covering empirically for Listeria monocytogenes in all patients with penicillin allergy with suspected bacterial meningitis. The case also shows that it is possible to have significant infection and inflammation even with negative microscopy, negative cultures, and negative broad range polymerase chain reaction in cases of Listeria meningitis. Follow-up spinal taps can be necessary to detect the presence of Listeria monocytogenes.

  13. Lymph node culture

    Science.gov (United States)

    Culture - lymph node ... or viruses grow. This process is called a culture. Sometimes, special stains are also used to identify specific cells or microorganisms before culture results are available. If needle aspiration does not ...

  14. Blood culture

    Science.gov (United States)

    Culture - blood ... A blood sample is needed . The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  15. callus culture

    African Journals Online (AJOL)

    steve

    and KH2PO4 -NH4NO3 depletion was fatal to grape cells and resulted in formation ... Individual phenolic compounds found in the grape cell cultures were ...... Characterization of in vitro anthocyanin-producing sour cherry ... Process Biochem.

  16. The cultural competence of health care professionals: conceptual analysis using the results from a national pilot study of training and assessment

    NARCIS (Netherlands)

    Prescott-Clements, L.; Schuwirth, L.; Vleuten, C.P.M. van der; Hurst, Y.; Whelan, G.; Gibb, E.; Rennie, J.

    2013-01-01

    The cultural competence of health professionals affects the satisfaction with, and outcomes of, patient care. Patient-centered cultural training was implemented with 76 trainees. Four months later, they were assessed using standardized patient scenarios and their performance compared to a control gr

  17. Conantokins inhibit NMDAR-dependent calcium influx in developing rat hippocampal neurons in primary culture with resulting effects on CREB phosphorylation.

    Science.gov (United States)

    Huang, Luoxiu; Balsara, Rashna D; Sheng, Zhenyu; Castellino, Francis J

    2010-10-01

    The effects of conantokin (con)-G, con-R[1-17], and con-T on ion flow through N-methyl-D-aspartate receptor (NMDAR) ion channels were determined in cultured primary rat hippocampal neurons. The potency of con-G diminished, whereas inhibition by con-R[1-17] and con-T did not change, as the neurons matured. Con-G, con-R[1-17], and con-T effectively diminished NMDA-induced Ca(2+) influx into the cells. A similar age-dependent decrease in con-G-mediated inhibition of the amplitude of spontaneous excitatory postsynaptic currents (sEPSCs) was observed, compared to con-R[1-17] and con-T. The effects of the conantokins on NMDA-induced cAMP response element-binding protein (CREB) phosphorylation in immature (DIV 9) and mature (DIV 16) neurons showed that, at DIV 9, con-G, con-R[1-17], and con-T inhibited NMDA-mediated P-CREB levels, whereas in DIV 16 neurons the conantokins did not inhibit overall levels of NMDA-induced P-CREB. In contrast, P-CREB levels were enhanced through inhibition of the protein phosphatases, PP1 and PP2B (calcineurin). This ability of conantokins to sustain CREB phosphorylation can thus enhance neuronal survival and plasticity.

  18. Factors Associated to a Reactive Result of Rapid-HIV Test in Socio-culturally Adapted Services in Primary Care in Spain.

    Science.gov (United States)

    Esteban-Vasallo, M D; Domínguez-Berjón, M F; García-Riolobos, C; Morán-Arribas, M; Rico-Bermejo, J; Collado-González, S; Aguirre Martín-Gil, R; López Arilla, G; Ultra-Berzosa, J; Jiménez-García, R

    2015-12-01

    The Services of Prevention and Early diagnosis of HIV in Madrid (Spain) are set in selected primary care centers. Cultural mediators targeted to vulnerable groups (economic immigrants, MSM, sex workers…) perform risk assessment and counselling. Between 2010 and 2014 they performed 6 039 rapid-HIV test, 27.8 % in MSM, 41.2 % in men who have sex exclusively with women (MSW) and 31.0 % in women; 35.7 % in immigrants, mainly from Latin America. A reactive result was more common among MSM (6.0 %) compared to women (0.6 %) and MSW (0.5 %). In MSM it was associated to being immigrant and to antecedents of sexually transmitted infections (STI). Among MSW the factors associated to a reactive result were: seropositivity of sexual partner and heroine consumption, and in women: infrequent use of condoms, seropositivity of sexual partner and antecedents of STI. Preventive interventions to reduce risk of HIV transmission and for early detection should be adapted and targeted to high risk population.

  19. National Cultures and Human Development Index

    Directory of Open Access Journals (Sweden)

    Edvard Konrad

    2012-12-01

    Full Text Available This paper explores the relationships between basic cultural characteristics of countries and some economic indexes. As cultural characteristics, the data from The Global Leadership and Organizational Behavior Effectiveness Research Program (GLOBE about the 9 cultural dimensions for 60 countries were used. Two facets of cultural dimensions were measured: the perceptions of actual practices and the perceptions of preferred values. On the other hand, the data about different economic indexes were taken from archival sources such as Human Development Report. Results show that some cultural practices and preferences are related to the development of countries as measured by Human Development Index (HDI. The implications of these results are discussed.

  20. Culture and Happiness.

    Science.gov (United States)

    Ye, Dezhu; Ng, Yew-Kwang; Lian, Yujun

    Culture is an important factor affecting happiness. This paper examines the predictive power of cultural factors on the cross-country differences in happiness and explores how different dimensions of cultural indices differ in their effects on happiness. Our empirical results show that the global leadership and organizational behavior effectiveness nine culture indices are all significantly related with happiness. Out of these nine indices, power distance (PDI) and gender egalitarianism (GEI) play the most important and stable role in determining subjective well-being (SWB). We further examine the relative importance of the various variables in contributing to the R-squared of the regression. The results show that PDI is the most important, accounting for 50 % of the contributions to R-squared of all variables, or equalling the combined contributions of income, population density and four other traditional variables. The contribution of GEI is 37.1 %, also well surpassing other variables. Our results remain robust even taking account of the different data for culture and SWB.

  1. A paradox of cumulative culture.

    Science.gov (United States)

    Kobayashi, Yutaka; Wakano, Joe Yuichiro; Ohtsuki, Hisashi

    2015-08-21

    Culture can grow cumulatively if socially learnt behaviors are improved by individual learning before being passed on to the next generation. Previous authors showed that this kind of learning strategy is unlikely to be evolutionarily stable in the presence of a trade-off between learning and reproduction. This is because culture is a public good that is freely exploited by any member of the population in their model (cultural social dilemma). In this paper, we investigate the effect of vertical transmission (transmission from parents to offspring), which decreases the publicness of culture, on the evolution of cumulative culture in both infinite and finite population models. In the infinite population model, we confirm that culture accumulates largely as long as transmission is purely vertical. It turns out, however, that introduction of even slight oblique transmission drastically reduces the equilibrium level of culture. Even more surprisingly, if the population size is finite, culture hardly accumulates even under purely vertical transmission. This occurs because stochastic extinction due to random genetic drift prevents a learning strategy from accumulating enough culture. Overall, our theoretical results suggest that introducing vertical transmission alone does not really help solve the cultural social dilemma problem. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Native Culture Issues in Cross-cultural Teaching

    Institute of Scientific and Technical Information of China (English)

    万欣

    2012-01-01

      The bi-direction of cross-cultural communication determines culture teaching should include both target culture and native culture. Currently, however, mere emphasis of target culture with ignoring native culture has resulted in“two-skin”phenomenon and“aphasia of Chinese culture”. Therefore, this paper aims to underline native culture teaching, to explore proper techniques for native culture teaching, to achieve integration of target culture and native culture, to enhance students’expressive competence in native culture and finally to carry out effective cross-cultural communication.

  3. Culture Matters

    Directory of Open Access Journals (Sweden)

    Gillian Warner-Søderholm

    2012-12-01

    Full Text Available Whether managers are concerned with financial issues, marketing, or human resource management (HRM, cultural values and practices do matter. The purpose of this article is to understand Norwegian managers’ cultural values within the cross-cultural landscape of her neighbors in the “Scandinavian cluster.” Clearly, subtle but disturbing differences may surface even when representatives from similar cultures work together. As a follow on from the GLOBE project, data based on the GLOBE instrument were collected on culture and communication values in Norway from 710 Norwegian middle managers for this present study. Although the Scandinavian cultures appear ostensibly similar, the results illustrate that research can reveal subtle but important cultural differences in nations that are similar yet dissimilar. All three Scandinavian societies appear intrinsically egalitarian; they appear to value low Power Distance, directness, and consensus in decision making and to promote Gender Egalitarianism. Nevertheless, there are significant differences in the degrees of commitment to these values by each individual Scandinavian partner. These differences need to be understood and appreciated to avoid misunderstandings.

  4. Cultural tourism and tourism cultures

    DEFF Research Database (Denmark)

    Ooi, Can-Seng

    Presenting a comprehensive and dynamic understanding of cultural tourism, this volume examines cultural mediators and how they help tourists appreciate foreign cultures. It also shows how tourism experiences are strategically crafted by mediators, the complexity of the mediation process, and how...... various products are mediated differently. A number of different products are investigated, including destination brand identities, "living" cultures and everyday life, art and history. The author illustrates his arguments by comparing the tourism strategies of Copenhagen and Singapore, and demonstrates...... how tourism is an agent for social change. The author also offers an original and refreshing way of understanding tourist behaviour through the concept of the "versatile tourist". The book's empirical cases and dialogic framework provide new and deep insights into tourism activities. In his...

  5. Does horizontal transmission invalidate cultural phylogenies?

    Science.gov (United States)

    Greenhill, Simon J; Currie, Thomas E; Gray, Russell D

    2009-06-22

    Phylogenetic methods have recently been applied to studies of cultural evolution. However, it has been claimed that the large amount of horizontal transmission that sometimes occurs between cultural groups invalidates the use of these methods. Here, we use a natural model of linguistic evolution to simulate borrowing between languages. The results show that tree topologies constructed with Bayesian phylogenetic methods are robust to realistic levels of borrowing. Inferences about divergence dates are slightly less robust and show a tendency to underestimate dates. Our results demonstrate that realistic levels of reticulation between cultures do not invalidate a phylogenetic approach to cultural and linguistic evolution.

  6. 2009 Hands Across Pacific Show Held

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    <正>The 2009 Hands Across the Pacific Show co-sponsored by the Canadian International Cultural Exchanges (CICEX) and Hunan Provincial People’s Association for Friendship with Foreign Countries(HPPAFFC) was held respectively in the Tower Hall of Tokyo,Japan from July 2 to 7 and in the Hunan Provincial Museum from August 1 to 5.Jing Dunquan,Vice President of the

  7. Cultural competence in medical education

    DEFF Research Database (Denmark)

    Sørensen, Janne; Jervelund, Signe Smith; Nørredam, Marie Louise

    2017-01-01

    the survey, and 199 responded. The response rate is 14%. Data were analysed through descriptive calculations, and answers to open-ended questions were coded using content analysis. Results: Results showed that 82.4% of the informants agreed or strongly agreed that the medical education programme should...... in receiving training on cultural competence. Conclusions: Generally, there is interest in and acknowledgement of the importance of cultural competence in Danish medical education among teachers at the University of Copenhagen. This creates an opportunity to implement cultural competence in the medical...

  8. Proteome Analyses of Strains ATCC 51142 and PCC 7822 of the Diazotrophic Cyanobacterium Cyanothece sp under Culture Conditions Resulting in Enhanced H-2 Production

    Energy Technology Data Exchange (ETDEWEB)

    Aryal, Uma K.; Callister, Stephen J.; Mishra, Sujata; Zhang, Xiaohui; Shutthanandan, Janani I.; Angel, Thomas E.; Shukla, Anil K.; Monroe, Matthew E.; Moore, Ronald J.; Koppenaal, David W.; Smith, Richard D.; Sherman, Louis

    2013-02-01

    Cultures of the cyanobacterial genus Cyanothece have been shown to produce high levels of biohydrogen. These strains are diazotrophic and undergo pronounced diurnal cycles when grown under N2-fixing conditions in light-dark cycles. We seek to better understand the way in which proteins respond to these diurnal changes and we performed quantitative proteome analysis of Cyanothece ATCC 51142 and PCC 7822 grown under 8 different nutritional conditions. Nitrogenase expression was limited to N2-fixing conditions, and in the absence of glycerol, nitrogenase gene expression was linked to the dark period. However, glycerol induced expression of nitrogenase during part of the light period, together with cytochrome c oxidase (Cox), glycogen phosphorylase (Glp), and glycolytic and pentose-phosphate pathway (PPP) enzymes. This indicated that nitrogenase expression in the light was facilitated via higher respiration and glycogen breakdown. Key enzymes of the Calvin cycle were inhibited in Cyanothece ATCC 51142 in the presence of glycerol under H2 producing conditions, suggesting a competition between these sources of carbon. However, in Cyanothece PCC 7822, the Calvin cycle still played a role in cofactor recycling during H2 production. Our data comprise the first comprehensive profiling of proteome changes in Cyanothece PCC 7822, and allows an in-depth comparative analysis of major physiological and biochemical processes that influence H2-production in both the strains. Our results revealed many previously uncharacterized proteins that may play a role in nitrogenase activity and in other metabolic pathways and may provide suitable targets for genetic manipulation that would lead to improvement of large scale H2 production.

  9. Proteome Analyses of Strains ATCC 51142 and PCC 7822 of the Diazotrophic Cyanobacterium Cyanothece sp. under Culture Conditions Resulting in Enhanced H2 Production

    Science.gov (United States)

    Aryal, Uma K.; Callister, Stephen J.; Mishra, Sujata; Zhang, Xiaohui; Shutthanandan, Janani I.; Angel, Thomas E.; Shukla, Anil K.; Monroe, Matthew E.; Moore, Ronald J.; Koppenaal, David W.; Smith, Richard D.

    2013-01-01

    Cultures of the cyanobacterial genus Cyanothece have been shown to produce high levels of biohydrogen. These strains are diazotrophic and undergo pronounced diurnal cycles when grown under N2-fixing conditions in light-dark cycles. We seek to better understand the way in which proteins respond to these diurnal changes, and we performed quantitative proteome analysis of Cyanothece sp. strains ATCC 51142 and PCC 7822 grown under 8 different nutritional conditions. Nitrogenase expression was limited to N2-fixing conditions, and in the absence of glycerol, nitrogenase gene expression was linked to the dark period. However, glycerol induced expression of nitrogenase during part of the light period, together with cytochrome c oxidase (Cox), glycogen phosphorylase (Glp), and glycolytic and pentose phosphate pathway (PPP) enzymes. This indicated that nitrogenase expression in the light was facilitated via higher levels of respiration and glycogen breakdown. Key enzymes of the Calvin cycle were inhibited in Cyanothece ATCC 51142 in the presence of glycerol under H2-producing conditions, suggesting a competition between these sources of carbon. However, in Cyanothece PCC 7822, the Calvin cycle still played a role in cofactor recycling during H2 production. Our data comprise the first comprehensive profiling of proteome changes in Cyanothece PCC 7822 and allow an in-depth comparative analysis of major physiological and biochemical processes that influence H2 production in both strains. Our results revealed many previously uncharacterized proteins that may play a role in nitrogenase activity and in other metabolic pathways and may provide suitable targets for genetic manipulation that would lead to improvement of large-scale H2 production. PMID:23204418

  10. Molecular characterization of a dechlorinating community resulting from in situ biostimulation in a trichloroethene-contaminated deep, fractured basalt aquifer and comparison to a derivative laboratory culture.

    Science.gov (United States)

    Macbeth, Tamzen W; Cummings, David E; Spring, Stefan; Petzke, Lynn M; Sorenson, Kent S

    2004-12-01

    Sodium lactate additions to a trichloroethene (TCE) residual source area in deep, fractured basalt at a U.S. Department of Energy site have resulted in the enrichment of the indigenous microbial community, the complete dechlorination of nearly all aqueous-phase TCE to ethene, and the continued depletion of the residual source since 1999. The bacterial and archaeal consortia in groundwater obtained from the residual source were assessed by using PCR-amplified 16S rRNA genes. A clone library of bacterial amplicons was predominated by those from members of the class Clostridia (57 of 93 clones), of which a phylotype most similar to that of the homoacetogen Acetobacterium sp. strain HAAP-1 was most abundant (32 of 93 clones). The remaining Bacteria consisted of phylotypes affiliated with Sphingobacteria, Bacteroides, Spirochaetes, Mollicutes, and Proteobacteria and candidate divisions OP11 and OP3. The two proteobacterial phylotypes were most similar to those of the known dechlorinators Trichlorobacter thiogenes and Sulfurospirillum multivorans. Although not represented by the bacterial clones generated with broad-specificity bacterial primers, a Dehalococcoides-like phylotype was identified with genus-specific primers. Only four distinct phylotypes were detected in the groundwater archaeal library, including predominantly a clone affiliated with the strictly acetoclastic methanogen Methanosaeta concilii (24 of 43 clones). A mixed culture that completely dechlorinates TCE to ethene was enriched from this groundwater, and both communities were characterized by terminal restriction fragment length polymorphism (T-RFLP). According to T-RFLP, the laboratory enrichment community was less diverse overall than the groundwater community, with 22 unique phylotypes as opposed to 43 and a higher percentage of Clostridia, including the Acetobacterium population. Bioreactor archaeal structure was very similar to that of the groundwater community, suggesting that methane is

  11. Cultural differences in risk

    Directory of Open Access Journals (Sweden)

    Do-Yeong Kim

    2010-08-01

    Full Text Available We compared South Koreans with Australians in order to characterize cultural differences in attitudes and choices regarding risk, at both the individual and group levels. Our results showed that Australians, when assessed individually, consistently self-reported higher preference for risk than South Koreans, regardless of gender. The data revealed that South Koreans, regardless of gender composition, were willing to take greater risks when making decisions in group decision-making situations than when they were alone. This is a different pattern from that seen in the Australian sample, in which a risky shift was noted only among males. This difference was attributed to the influence of various cultural orientations (independent vs. interdependent relationship styles. This study also provides a discussion of the implications of these results in terms of cultural differences in attitudes and decisions regarding risk.

  12. On the origin of the two cultures in 19th-century Europe (Cultural impact of a new view of the relation between man and nature resulting from the "Darwinian revolution")

    NARCIS (Netherlands)

    Van Berkel, K

    2000-01-01

    The separation between nature, as the domain of scientific laws, and culture, as the domain of human values, is potentially already present in the seventeenth century. The idea of a benevolent purpose in nature, the so-called physico-theology, for a long time helped to accommodate the findings of sc

  13. Absorbing the Culture Shock

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Participants at a forum on communication between cultures generate ideas on how China can deal with its growing cultural deficit Five months ago, Ding Wei, Assistant Minister of Culture, described China's deficit in international cultural trade as "huge" at a press conference held by the State Council Information Office. "Our statistics years ago showed that the ratio of imports of cultural products to exports was 10 to 1," he

  14. Cultural Capital in Context: Heterogeneous Returns to Cultural Capital Across Schooling Environments

    DEFF Research Database (Denmark)

    Andersen, Ida Gran; Jæger, Mads Meier

    2015-01-01

    This paper tests two competing explanations of differences in returns to cultural capital across schooling environments: Cultural reproduction (cultural capital yields a higher returns in high-achieving environments than in low-achieving ones) and cultural mobility (cultural capital yields higher...... returns in low-achieving environments). Using multilevel mixture models, empirical results from analyses based on PISA data from three countries (Canada, Germany, and Sweden) show that returns to cultural capital tend to be higher in low-achieving schooling environments than in high-achieving ones....... These results principally support the cultural mobility explanation and suggest that research should pay explicit attention to the institutional contexts in which cultural capital is converted into educational success....

  15. Cultural Capital in Context: Heterogeneous Returns to Cultural Capital Across Schooling Environments

    DEFF Research Database (Denmark)

    Andersen, Ida Gran; Jæger, Mads Meier

    2015-01-01

    returns in low-achieving environments). Using multilevel mixture models, empirical results from analyses based on PISA data from three countries (Canada, Germany, and Sweden) show that returns to cultural capital tend to be higher in low-achieving schooling environments than in high-achieving ones......This paper tests two competing explanations of differences in returns to cultural capital across schooling environments: Cultural reproduction (cultural capital yields a higher returns in high-achieving environments than in low-achieving ones) and cultural mobility (cultural capital yields higher....... These results principally support the cultural mobility explanation and suggest that research should pay explicit attention to the institutional contexts in which cultural capital is converted into educational success....

  16. Research on the Pueblo culture settlement system from the North American Southwest: Results of the Sand Canyon-Castle Rock Community Archaeological Project

    Directory of Open Access Journals (Sweden)

    Radosław Palonka

    2015-02-01

    Full Text Available Investigating ancient Pueblo culture from the North American Southwest is challenging task involving cooperationof scientists from different disciplines, mainly archaeology, history, anthropology, and linguistics. There isalso a large body of information in native oral tradition that has enormous potential for enriching our knowledgeof the past and our understanding of how Pueblo societies functioned. The paper focuses on one of the mostintriguing periods of Pueblo Indians culture, the thirteenth century A.D., in the central Mesa Verde region onpresent Utah-Colorado border. It was the time of great development of Pueblo societies and close to the centuryfall of the settlement system and total migration from the area to what is present-day Arizona and New Mexico.One of the projects in the area is Sand Canyon-Castle Rock Community Archaeological Project. The projectfocuses on analysis and reconstruction of the settlement structure and socio-cultural changes that took placein Pueblo culture during the thirteenth century A.D. in Sand Canyon, Rock Creek Canyon and several othersmall canyons located in one subarea within the Mesa Verde region, Colorado.

  17. Application of a quality by design approach to the cell culture process of monoclonal antibody production, resulting in the establishment of a design space.

    Science.gov (United States)

    Nagashima, Hiroaki; Watari, Akiko; Shinoda, Yasuharu; Okamoto, Hiroshi; Takuma, Shinya

    2013-12-01

    This case study describes the application of Quality by Design elements to the process of culturing Chinese hamster ovary cells in the production of a monoclonal antibody. All steps in the cell culture process and all process parameters in each step were identified by using a cause-and-effect diagram. Prospective risk assessment using failure mode and effects analysis identified the following four potential critical process parameters in the production culture step: initial viable cell density, culture duration, pH, and temperature. These parameters and lot-to-lot variability in raw material were then evaluated by process characterization utilizing a design of experiments approach consisting of a face-centered central composite design integrated with a full factorial design. Process characterization was conducted using a scaled down model that had been qualified by comparison with large-scale production data. Multivariate regression analysis was used to establish statistical prediction models for performance indicators and quality attributes; with these, we constructed contour plots and conducted Monte Carlo simulation to clarify the design space. The statistical analyses, especially for raw materials, identified set point values, which were most robust with respect to the lot-to-lot variability of raw materials while keeping the product quality within the acceptance criteria.

  18. COMMON MENTAL-DISORDERS AND DISABILITY ACROSS CULTURES - RESULTS FROM THE WHO COLLABORATIVE STUDY ON PSYCHOLOGICAL-PROBLEMS IN GENERAL HEALTH-CARE

    NARCIS (Netherlands)

    ORMEL, J; VONKORFF, M; USTUN, TB; PINI, S; KORTEN, A; OLDEHINKEL, T

    1994-01-01

    Objective.-To examine the impact of common mental illness on functional disability and the cross-cultural consistency of this relationship while controlling for physical illness. A secondary objective was to determine the level of disability associated with specific psychiatric disorders. Design.-A

  19. "Less Clicking, More Watching": Results from the User-Centered Design of a Multi-Institutional Web Site for Art and Culture.

    Science.gov (United States)

    Vergo, John; Karat, Clare-Marie; Karat, John; Pinhanez, Claudio; Arora, Renee; Cofino, Thomas; Riecken, Doug; Podlaseck, Mark

    This paper summarizes a 10-month long research project conducted at the IBM T.J. Watson Research Center aimed at developing the design concept of a multi-institutional art and culture web site. The work followed a user-centered design (UCD) approach, where interaction with prototypes and feedback from potential users of the web site were sought…

  20. COMMON MENTAL-DISORDERS AND DISABILITY ACROSS CULTURES - RESULTS FROM THE WHO COLLABORATIVE STUDY ON PSYCHOLOGICAL-PROBLEMS IN GENERAL HEALTH-CARE

    NARCIS (Netherlands)

    ORMEL, J; VONKORFF, M; USTUN, TB; PINI, S; KORTEN, A; OLDEHINKEL, T

    1994-01-01

    Objective.-To examine the impact of common mental illness on functional disability and the cross-cultural consistency of this relationship while controlling for physical illness. A secondary objective was to determine the level of disability associated with specific psychiatric disorders. Design.-A

  1. Communication as a mechanism for cultural integration.

    Science.gov (United States)

    Backstrom, Tomas; Hagstrom, Tom; Goransson, Susanna

    2013-01-01

    Providing autonomy for employees ensures innovation competence if balanced by integration into the organization. The aim of this article is to study processes leading to the integration of employees into the company culture. The two research questions are: What makes the culture of a work group similar to the company culture? How is a work group culture constructed? Theories that are employed concern culture as an organizing structure emerging in the interaction, company culture as a way to exert control, and social networks as a way to describe the interaction. Empirical data come from a merchant bank from which 105 respondents from ten work groups answered questions about their communication and their integration into the company culture. The results show that the sub-culture of the group emerges in communication between members of the group. There seems to be a self-reinforcing spiral between collegial talk, especially about goals, plans and changes at the work place, and cultural integration. All members of a group should be included in this communication to create a strong culture. The value system of the supervisor strongly influences the sub-culture of the work group. Appointing supervisors with values that correspond to the company culture and provide for employee communications is thus central for organizations using culture as a tool for control.

  2. 论泛娱乐时代电视真人秀节目的文化自觉——以职场真人秀《职来职往》为例%Discussion on Cultural Consciousness of Reality TV Show in the Pan-entertainment Era

    Institute of Scientific and Technical Information of China (English)

    郝伟

    2011-01-01

    随着中国电视媒体泛娱乐化倾向的扩大,煊赫一时的"真人秀"节目也逐渐脱离其精神价值,陷入发展的文化困境。因此,探索一条适合中国电视文化语境的,健康、可持续的发展之路至关重要。当下收视与口碑双赢的职场真人秀《职来职往》为我们提供了有价值的发展思路。%With the overflow of pan-entertainment phenomenon in the Chinese TV programs,the used-to-be popular 'Reality TV Shows' gradually reveal the trend of vulgarization and get into the cultural dilemma of development.Therefore,it is of utmost importance to explore a healthy,sustainable way of development which fits the Chinese TV cultural context.The job-hunting reality show 'Jobs Come Jobs go',which enjoys a high audience rating and positive comments,provides a beneficial reference to us.

  3. 249例前列腺液细菌培养及药敏结果分析%Analysis of bacterial culture and drug sensitivity results of 249 prostate lfuid samples

    Institute of Scientific and Technical Information of China (English)

    杨彦磊; 王瑞; 张卫星

    2014-01-01

    目的:分析前列腺液细菌培养结果,探索细菌感染在慢性细菌性前列腺炎病因学中的意义,对临床慢性细菌性前列腺炎的治疗提供参考。方法嘱前列腺炎患者停用抗生素3d以上,清洗尿道外口,按摩前列腺液作细菌培养及药敏试验。结果249份前列腺液标本中,98份细菌培养阳性,阳性率39.4%。检出细菌98株,其中革兰阳性菌92株,占93.9%,革兰阴性杆菌6株,占6.1%。78株葡萄球菌的体外药敏试验结果显示,仅对万古霉素、利奈唑胺和替考拉宁敏感,而对青霉素、阿奇霉素、红霉素和克拉霉素全部耐药。对其他种类抗生素亦有不同程度的耐药。6株革兰阴性杆菌药敏结果显示,对常见的抗生素均敏感。结论革兰阳性葡萄球菌及棒状杆菌可能在慢性细菌性前列腺炎病原学中占有一定比例,抗生素的耐药性应引起高度重视。%Objective To analyze bacterial culture results of prostate fluid samples and explore the etiological significance of bacterial infection for clinical treatment of chronic bacterial prostatitis. Methods Patients with prostatitis were asked to stop antibiotics treatment for more than 3 days and clean their external orifice of urethra prior to prostatic fluid collecting. Prostatic fluid samples were collected for bacterial culture and drug sensitivity test. Results In all 249 prostatic fluid samples, 98 of them showed positive result in bacterial culture. The positive rate was 39.4%, including 92 strains of Gram-positive Bacterial strains, accounting for 93.9%, 6 strains of Gram-negative bacterial strains, accounting for 6.1%. Drug sensitivity results from 78 cases of staphylococcal strains showed they were sensitive to vancomycin, linezolid and teicoplanin, but resistant to penicilinc, azithromycin, erythromycin, clarithromycin and other antibiotics. Drug sensitivity results from 6 strains of gram negative bacterial showed they were

  4. Reflection on cultural value of emotional consumption in Reality Show programs:a case study of Fighting for Her%真人秀节目情感消费的文化价值思考——以江苏卫视《为她而战》为例

    Institute of Scientific and Technical Information of China (English)

    葛靖

    2015-01-01

    现代社会公共交往空间的压缩带来公众情感需求的匮乏,真人秀作为推崇与电视观众情感共鸣的媒介产品,无疑是消费文化和媒介文化结合的产物,但真人秀节目纯粹消费明星情感是一种双向消费,对电视媒体而言不仅是极端娱乐化的问题,亦有忽略情感本身的现实基础问题,电视上的秀终究是为了迎合受众所制造出的虚幻幕剧.随着受众批判思维的拓进,以网络和电视为代表的新时代受众便会产生窥视不属于自身阶层生活的失落感和迷失感.文章基于对江苏卫视《为她而战》节目情感消费逻辑的梳理,以情感的蛰伏期、认同期、崩塌期作为发展脉络,深度剖析真人秀节目情感消费的文化价值,并以此为范本反思当下真人秀节目的消费文化怪圈,提出感官刺激和异化审美下真人秀节目制作逻辑的肇因,以期能纠偏真人秀节目的发展歪路.%The compression of public communication space in modern society brings about the deficiency of public emotional needs. The Reality Show program on TV,a media product that promotes the echoes of the audience,is un-doubtedly a combination of consumption culture and media culture. The consumption of the stars'emotion in Real-ity Show,however,is problematic in that it has a tendency of extreme entertainment for the television media,and it caters excessively to the audience at the expense of emotion itself. With the development of the audience's critical thinking,the new audience stimulated by the network and television tends to have the sense of loss or the sense of disorientation after getting a glimpse of the life that does not belong to his own social stratum. In this paper,we take the Reality Show program Fighting for Her from Jiangsu Satelite Television as a sample to analyze the cultural value of emotion consumption in the three periods of emotion consumption. Based on the case study of Fighting for Her,we reflect the

  5. Mayan Culture

    DEFF Research Database (Denmark)

    Hervik, Peter Bent

    1992-01-01

    The social categories « Maya » and « mestizo » habe been applied to denote the Yucatec Mayan people in Mexico. The A. examines the cluster of perceived attributes (schemata) evoked by the terms and how they relate to each other. He shows that there is an incongruency between them along the lines ...... of local and academic categorization, which is an implication of the different social spaces in which they arise. In spite of the incongruency and the cultural plurality evoked by their usage, the A. argues that the people of Yucatec share a single culture....

  6. Influence of organisational culture on company performance

    Directory of Open Access Journals (Sweden)

    R.M. Yusuff

    2008-12-01

    Full Text Available Purpose: The lack of organizational effort to assess cultural compatibility or fit prior to the engagement offirms has contributed to the failure of several mergers and acquisition. A Korean public listed company withmanufacturing plants in Malaysia and New Zealand found that the performances of the newly acquired plantswere significantly lower than the manufacturing plants in Korea.Design/methodology/approach: In this study, the influence of national culture on organizational culture andthe effect on the organizational performance was conducted on 6 manufacturing plants in Korea (2, Malaysia(3 and New Zealand (1. Hofstede’s culture dimensions were used to determine culture diversity between themanufacturing plants.Findings: The values survey module was used to calculate the index scores on five dimensions of national valuesystem as components of national cultures: power distance, Individualism, Masculinity, Uncertainty avoidanceand Long term orientation.Practical implications: The model of culture fit assesses the dimensions of socio-cultural environment, internalwork culture and HRM practices. The resulting multiple regression analysis showed that there are culturaldiversities between the manufacturing plants and national culture does influence organizational performance.Originality/value: The results also showed that a dimension of internal work culture does influenceorganizational performance.

  7. Surveys of organizational culture and safety culture in nuclear power

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Walter S. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2000-07-30

    The results of a survey of organizational culture at a nuclear power plant are summarized and compared with those of a similar survey which has been described in the literature on ''high-reliability organizations''. A general-purpose cultural inventory showed a profile of organizational style similar to that reported in the literature; the factor structure for the styles was also similar to that of the plant previously described. A specialized scale designed to measure ''safety culture'' did not distinguished among groups within the organization that would be expected to differ.

  8. Simultaneous knockdown of p18INK4C, p27Kipl and MAD1 via RNA interference results in the expansion of long-term culture-initiating cells of murine bone mar-row cells in vitro

    Institute of Scientific and Technical Information of China (English)

    Yan-Yi Wang; Yong Yang; Qingyong Chen; Jianping Yu; Yongzhong Hou; Lizhen Han; Jun He; Demin Jiao; Huihui Yu

    2008-01-01

    A combination of extrinsic hematopoietic growth regulators,such as stem cell factor (SCF), interleukin (IL)-3 and IL-6,can induce division of quiescent hematopoietic stem cells (HSCs), but it usually impairs HSCs' serf-renewal ability.However,intrinsic negative cell cycle regulators,such as p18INK4C(p18),p27Kip1(p27)and MAD1,can regulate the self-renewal of HSCs.It is unknown whether the removal of some extrinsic regulators and the knockdown of intrinsic negative cell cycle regulators via RNA interference (RNAi)induce ex vivo expansion of the HSCs.To address this question,a lentiviral vector-based RNAitool was developed to produce two copies of small RNA that target multiple genes to knock-down the intrinsic negative cell cycle regulators p18,p27 and MAD1.Colony-forming cells,long-term culture-initiating cell(LTC-IC)and engraftment assays were used to evaluate the effects of extrinsic and intrinsic regulators.Results showed that the medium with only SCF,but without IL-3 and IL-6,could maintain the sca-1+c-kit+bone marrow cells with high LTC-IC frequency and low cell division. However,whenthe sca-1+c-kit+bone marrow cells were cultured in a medium with only SCF and simultaneously knocked down the expression of p18,p27and MAD1 via the lentiviral vector-based RNAI,the cells exhibited both high LTC-IC frequency and high cell division,though engraftment failed.Thus,the simultaneous lnockdown of p18,p27and MAD1 with a medium of only SCF can induce LTC-IC expansion despite the loss of engraftment ability.

  9. Expression of an Antirrhinum dihydroflavonol reductase gene results in changes in condensed tannin structure and accumulation in root cultures of Lotus corniculatus (bird's foot trefoil).

    Science.gov (United States)

    Bavage, A D; Davies, I G; Robbins, M P; Morris, P

    1997-11-01

    Condensed tannins (proanthocyanidins) are an important factor in the nutritive and dietary quality of many forage crops. We report here experiments aimed at altering the levels and monomer composition of condensed tannins (CTs) in 'hairy root' cultures of Lotus corniculatus (bird's foot trefoil) using genetic manipulation. An Antirrhinum majus dihydroflavonol reductase (DFR) cDNA was expressed in sense in L. corniculatus and CT levels in transgenic root cultures were analysed. Two co-transformed lines were noted with decreased CT content relative to controls and these levels were comparable with antisense-DFR phenotypes. In ADFR10, a co-transformed line with the highest CT levels, CT structure was altered in a manner consistent with the substrate specificity of the introduced gene; that is an increase in pro-pelargonidin monomers noted after hydrolysis of CTs. RT-PCR confirmed the expression of endogenous DFR gene(s) in both putatively co-suppressed lines and also in ADFR10. Analysis of selected root culture lines indicated that the monomer composition of CTs did not change during growth and development but that levels of CTs varied in a regulated manner.

  10. Planning a Successful Tech Show

    Science.gov (United States)

    Nikirk, Martin

    2011-01-01

    Tech shows are a great way to introduce prospective students, parents, and local business and industry to a technology and engineering or career and technical education program. In addition to showcasing instructional programs, a tech show allows students to demonstrate their professionalism and skills, practice public presentations, and interact…

  11. Organizational Culture and the Financial Performance of Manufacturing Firms

    Directory of Open Access Journals (Sweden)

    Mahrooz Koochaki Golafzani

    2016-06-01

    Full Text Available This paper intends to examine the relationship between organizational culture and the financial performance of manufacturing firms in the province of Guilan (Iran. To do so, a statistical sample with the size of 247 firms located at industrial towns/parks in Guilan was selected. The required data was collected through questionnaire. Then, the relationship between organization culture, including the clan culture, adhocracy culture, market culture and hierarchy culture, and the financial performance was analyzed according to the proposed model and by using Spearman’s rank correlation coefficient. The results show that there is a significant positive relationship between clan culture (correlation coefficient: 0/65, adhocracy culture (0/63, market culture (0/68 and hierarchy culture (0/59 and the financial performance of manufacturing firms.

  12. Cultural determinants of email communication styles

    OpenAIRE

    Holtbrügge, Dirk; Weldon, Abigail; Rogers, Helen

    2014-01-01

    Based on a sample of 234 IT consulting and services professionals from various cultural backgrounds, this study investigates whether Edward T. Hall’s concept of culture can explain cultural differences in email communication. Although a vast array of intercultural research employs Hall’s concept in various communication settings, few take into account the computer-mediated communication technologies that now play an integral role in the operations of international companies. Our results show ...

  13. Cultural Influences on Play Style

    NARCIS (Netherlands)

    Bialas, Mateusz; Tekofsky, Shoshannah; Spronck, P.H.M.

    2014-01-01

    In general, video game researchers do not differentiate between players’ nationalities. Cultural theories, however, show that cultural differences concern numerous values, including values associated with interaction with media. We therefore ask the question whether there exist cross-cultural differ

  14. Risk Aversion in Game Shows

    DEFF Research Database (Denmark)

    2008-01-01

    We review the use of behavior from television game shows to infer risk attitudes. These shows provide evidence when contestants are making decisions over very large stakes, and in a replicated, structured way. Inferences are generally confounded by the subjective assessment of skill in some games......, and the dynamic nature of the task in most games. We consider the game shows Card Sharks, Jeopardy!, Lingo, and finally Deal Or No Deal. We provide a detailed case study of the analyses of Deal Or No Deal, since it is suitable for inference about risk attitudes and has attracted considerable attention....

  15. Measuring performance at trade shows

    DEFF Research Database (Denmark)

    Hansen, Kåre

    2004-01-01

    Trade shows is an increasingly important marketing activity to many companies, but current measures of trade show performance do not adequately capture dimensions important to exhibitors. Based on the marketing literature's outcome and behavior-based control system taxonomy, a model is built...... that captures a outcome-based sales dimension and four behavior-based dimensions (i.e. information-gathering, relationship building, image building, and motivation activities). A 16-item instrument is developed for assessing exhibitors perceptions of their trade show performance. The paper presents evidence...

  16. Project Organizations and Their Present and Preferred Culture

    Directory of Open Access Journals (Sweden)

    Lajos SZABO

    2015-12-01

    Full Text Available Although several research has investigated organizational culture (Schein, 2010; Alvesson, 2013, less research has been conducted on the comparison of present and preferred cultures in project context. This paper aims to fill this gap by focusing on project managers and on the investigation of the present and the preferred culture profile of their project organizations. Based on Cameron and Quinn's (2011 Competing Values Framework using the Organizational Culture Assessment Instrument a quantitative survey was conducted. The questionnaire used gathered data from project managers working in various industries and organizations. The sample consisted of 695 respondents. The empirical study by focusing on project organizations hypothesizes that four project organization groups can be revealed based on their dominant cultural orientation. To test this hypothesis cluster analysis was used. The study also hypothesizes that the present and preferred culture profiles of project organizations do not show significant difference. To prove this statement paired samples t-test was chosen. The results showed that instead of four groups of project organizations with one dominant culture type, there are only three project organizations with the domination of one culture type. Continuing the investigation with these three project organizations, the present and preferred project culture profiles were compared. The results showed that in all three project organizations there are differences between the present and preferred project culture profiles. These differences are manifested mainly by the change of the dominant culture type but the remaining culture types determining the culture profile of the project organizations also show differences.

  17. Value of children and fertility: Results from a cross-cultural comparative survey in eighteen areas in Asia, Africa, Europe and America.

    Science.gov (United States)

    Nauck, Bernhard

    2014-09-01

    For explaining cross-cultural differences in fertility behavior, this paper conjoins three complementary approaches: the 'demand'-based economic theory of fertility (ETF), a revised version of the 'supply'-based 'value-of-children' (VOC)-approach as a special theory of the general social theory of social production functions and the framing theory of variable rationality. A comprehensive model is specified that encompasses the variable efficiency of having children for the optimization of physical well-being and of social esteem of (potential) parents; it also accounts for the variable rationality of fertility decisions. The model is tested with a data set that comprises information on VOC and fertility of women within the social settings of 18 areas (Peoples Republic of China, North and South India, Indonesia, Palestine, Israel, Turkey, Ghana, South Africa, East and West Germany, the Czech Republic, France, Russia, Poland, Estonia, the United States and Jamaica). Latent class analysis is used to establish a measurement model for the costs and benefits of children and to analyze area differences by a two-level multinomial-model. Two-level Cox-regressions are used to estimate the effects of perceived costs and benefits of children, individual resources and context opportunities, with births of different parity as dependents. This simultaneous test in a cross-cultural context goes beyond the current state of fertility research and provides evidence about the cross-cultural validity of the model, the systematic effects of VOC on fertility and the changing rationality of fertility decisions during demographic transition and socio-economic change.

  18. Cultural Capital in Context:

    DEFF Research Database (Denmark)

    Andersen, Ida Gran; Jæger, Mads Meier

    This paper analyzes the extent to which the effect of cultural capital on academic achievement varies across high- and low-achieving schooling environments. We distinguish three competing theoretical models: Cultural reproduction (cultural capital yields higher returns in high-achieving schooling...... environments than in low-achieving ones), cultural mobility (cultural capital yields higher returns in low-achieving environments), and cultural resources (cultural capital yields the same returns in different environments). We analyze PISA data from six countries and find that returns to cultural capital tend...... to be higher in low-achieving schooling environments than in high-achieving ones. These results support the cultural mobility explanation and are in line with previous research suggesting that children from low-SES families benefit more from cultural capital than children from high-SES families....

  19. Tokyo Motor Show 2003; Tokyo Motor Show 2003

    Energy Technology Data Exchange (ETDEWEB)

    Joly, E.

    2004-01-01

    The text which follows present the different techniques exposed during the 37. Tokyo Motor Show. The report points out the great tendencies of developments of the Japanese automobile industry. The hybrid electric-powered vehicles or those equipped with fuel cells have been highlighted by the Japanese manufacturers which allow considerable budgets in the research of less polluting vehicles. The exposed models, although being all different according to the manufacturer, use always a hybrid system: fuel cell/battery. The manufacturers have stressed too on the intelligent systems for navigation and safety as well as on the design and comfort. (O.M.)

  20. DIAGNOSIS OF CULTURAL ORGANIZATIONS

    Directory of Open Access Journals (Sweden)

    ALBU MĂDĂLINA

    2015-04-01

    Full Text Available Cultural institution management is to direct the organization to a specific cultural profile purpose, namely production values esthetic sense, artistic, moral, spiritual, distribution, promotion of these values, protection and circulation of cultural heritage. In this regard, an analysis in the diagnosis cultural organizations aims to determine the main strengths and weaknesses, assess the potential and making recommendations focused on the root causes of failures and positive aspects. This paper presents considerations diligence activity Culture House "IL Caragiale "in Ploiesti. The mission of this organization is to contribute to the cultural development of the community by initiating projects and cultural programs, offer development programs and services to meet cultural needs, increase public access to diverse cultural life, providing a constant presence institution circuit local, national, European and international level. Conclusions drawn from the analysis shows that in a world of economic globalization, information and culture in a company in constant change, in a competitive market where there is information readily available means and leisure, but not cultural consistency in a social environment where interest in culture of people is declining, the situation of the population is impaired, the remuneration of staff working in the field of cultural education is demotivating, the funds allocated to culture have grown lately effectively lead a cultural institution is a challenge.

  1. Complexity of culture: the role of identity and context in bicultural individuals' body ideals.

    Science.gov (United States)

    Guan, Mei; Lee, Fiona; Cole, Elizabeth R

    2012-07-01

    Culture plays an important role in shaping body image, and people from different cultures have different beliefs about what constitutes the "ideal" body type. This study examines the relationship between culture and body ideals in Asian-American and Black-American women. Results from two studies show that subjective cultural identity and situational cultural cues had different relationships with body ideals. Among Asian-American women, identification with Asian culture was related to a thinner body ideal, but exposure to Asian cultural cues (relative to American cultural cues) was related to a thicker body ideal. Among Black-American women, identification with Black culture was related to a thicker body ideal, but exposure to Black cultural cues (relative to American cultural cues) was related to a thinner body ideal. These results have theoretical and practical implications for understanding how internal and external manifestations of culture can differentially influence body image.

  2. Risk Aversion in Game Shows

    DEFF Research Database (Denmark)

    Andersen, Steffen; Lau, Morten I.; Rutström, E. Elisabet

    2008-01-01

    , and the dynamic nature of the task in most games. We consider the game shows Card Sharks, Jeopardy!, Lingo, and finally Deal Or No Deal. We provide a detailed case study of the analyses of Deal Or No Deal, since it is suitable for inference about risk attitudes and has attracted considerable attention....

  3. Phyllodes tumor showing intraductal growth.

    Science.gov (United States)

    Makidono, Akari; Tsunoda, Hiroko; Mori, Miki; Yagata, Hiroshi; Onoda, Yui; Kikuchi, Mari; Nozaki, Taiki; Saida, Yukihisa; Nakamura, Seigo; Suzuki, Koyu

    2013-07-01

    Phyllodes tumor of the breast is a rare fibroepithelial lesion and particularly uncommon in adolescent girls. It is thought to arise from the periductal rather than intralobular stroma. Usually, it is seen as a well-defined mass. Phyllodes tumor showing intraductal growth is extremely rare. Here we report a girl who has a phyllodes tumor with intraductal growth.

  4. Pembrolizumab Shows Promise for NSCLC.

    Science.gov (United States)

    2015-06-01

    Data from the KEYNOTE-001 trial show that pembrolizumab improves clinical outcomes for patients with advanced non-small cell lung cancer, and is well tolerated. PD-L1 expression in at least 50% of tumor cells correlated with improved efficacy.

  5. Create a Polarized Light Show.

    Science.gov (United States)

    Conrad, William H.

    1992-01-01

    Presents a lesson that introduces students to polarized light using a problem-solving approach. After illustrating the concept using a slinky and poster board with a vertical slot, students solve the problem of creating a polarized light show using Polya's problem-solving methods. (MDH)

  6. On value and culture

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Value stands for the significance of things,and concepts of value are ideas,opinions and attitudes about what kind of things are significant.Studies on the concept of value cannot be separated from culture.Every society has its own distinct culture and concept of value shared by its people.The relationship between concept of value and culture shows that the building of the concept of value must be based on culture.Developing culture,providing excellent products of culture and better humanitarian environment are the premise for people's possession of the correct concept of value.

  7. Cultur(ally) Jammed: Culture Jams as a Form of Culturally Responsive Teaching

    Science.gov (United States)

    Martinez, Ulyssa

    2012-01-01

    Does the person become the name or does the name become the person? This question was asked by a participant of my culture jam entitled, "What's my name?" In this culture jam, I asked people to discern the name of a person based solely on their appearance and a list of possible names below their picture. This article aims to show how culture jams…

  8. Cultural Consumption of the Overseas Chinese Garden in the Process of Cross-cultural Communication

    Science.gov (United States)

    Zhai, L.

    2015-08-01

    When referring to the tangible cultural heritage, people tend to concern more about the conservation and research of the entity of the tangible heritage than the cross-cultural communication of the cultural heritage which is also one of the most important components of the preservation of the cultural heritage. As an exotic new born of the cultural heritage, the entity born from the cross-cultural communication inherits the properties of the cultural heritage on the one hand, and on the other hand generates diversities as a result of the differences based on social, cultural and environment. And the business model is one of the most important reasons for the production of diversities. There's no doubt that a good form of business model makes great significance to the cross-cultural communication. Therefore, the study of the business model of cultural heritage in the process of cross-cultural communication will not only contributes to the deeper understanding towards the phenomenon of the cultural heritage's cross-cultural communication, but also leads to the introspection to the tangible cultural heritage itself. In this way, a new kind of conservative notion could take form, and the goal of protecting cultural heritage could be achieved. Thus the Chinese Garden is a typical representation of the cultural heritage which makes great sense in the cross-cultural communication. As a kind of tangible cultural heritage, the Chinese gardens are well preserved in different regions in China. While the spirits of the Chinese garden carry forward through the construction of the Chinese gardens abroad during the cross-cultural communication. As a new kind of form of the cross-cultural communication of the cultural heritage, on the one hand, the Chinese gardens overseas built ever since China's Reform and Opening express creatively of the materialist and the spirituality of the traditional Chinese Garden, and on the other hand, those Chinese gardens overseas face all kinds of

  9. 对血培养实施全面质量控制前后结果分析%Analysis on results before and after implementing total quality control on blood culture

    Institute of Scientific and Technical Information of China (English)

    朱中华; 张茂海; 张桂花

    2016-01-01

    目的:通过对血培养实施全面质量控制措施,观察血培养实施前、后的结果变化。方法对进行质量控制措施前一年的血培养结果与进行质量控制措施后1年的结果进行比较分析。结果对血培养进行质量控制措施后,血培养的阳性率升高2.4%、污染率下降8.6%。结论通过对血培养实施全面质量控制可提高血培养阳性率、降低血培养污染率。污染菌的判定,一定结合实验室检查资料和患者临床情况。确保为临床提供准确、有效的抗菌药物。%Objective To observe the changes of the blood culture results before and after implementing the quality control on blood culture .Methods The blood culture results in 1 year before implementing the quality control and in 1 year after implementing the quality control were performed the contrastive analysis .Results After implementing the quality control on blood culture ,the positive rate of blood culture was elevated by 2 .4% and the pollution rate was decreased by 8 .6% after the quality control .Conclu‐sion Implementing the total quality control on blood culture can improve the positive rate of blood culture and reduces the pollu‐tion rate of blood culture .The determination of contamination bacteria must combine the laboratory detection data with the clinical situation for ensuring to provide accurate and effective antimicrobial agents for clinic .

  10. Reality show: um paradoxo nietzschiano

    Directory of Open Access Journals (Sweden)

    Ilana Feldman

    2011-01-01

    Full Text Available

    O fenômeno dos reality shows - e a subseqüente relação entre imagem e verdade - assenta-se sobre uma série de paradoxos. Tais paradoxos podem ser compreendidos à luz do pensamento do filósofo alemão Friedrich Nietzsche, que, através dos usos de formulações paradoxais, concebia a realidade como um mundo de pura aparência e a verdade como um acréscimo ficcional, como um efeito. A ficção é então tomada, na filosofia de Nietzsche, não em seu aspecto falsificante e desrealizador - como sempre pleiteou nossa tradição metafísica -, mas como condição necessária para que certa espécie de invenção possa operar como verdade. Sendo assim, a própria expressão reality show, através de sua formulação paradoxal, engendra explicitamente um mundo de pura aparência, em que a verdade, a parte reality da proposição, é da ordem do suplemento, daquilo que se acrescenta ficcionalmente - como um adjetivo - a show. O ornamento, nesse caso, passa a ocupar o lugar central, apontando para o efeito produzido: o efeito-de-verdade. Seguindo, então, o pensamento nietzschiano e sua atualização na contemporaneidade, investigaremos de que forma os televisivos “shows de realidade” operam paradoxalmente, em consonância com nossas paradoxais práticas culturais.

  11. The impact of yeast starter cultures on the microbial communities and volatile compounds in cocoa fermentation and the resulting sensory attributes of chocolate.

    Science.gov (United States)

    Batista, Nádia Nara; Ramos, Cíntia Lacerda; Dias, Disney Ribeiro; Pinheiro, Ana Carla Marques; Schwan, Rosane Freitas

    2016-02-01

    Theobroma cacao seeds are the main raw material for chocolate production. During their fermentation, a succession of microorganisms are responsible for the physicochemical changes occurring in the pulp and inside the beans. The aim of this study was to investigate the effects of yeast inoculation (Saccharomyces cerevisiae UFLA CA11, Pichia kluivery CCMA0237, and Hanseniaspora uvarum CCMA0236) on the profile of the volatile compounds and microbial communities in cocoa fermentation. The resulting chocolate was also evaluated by temporal dominance of sensations (TDS) analyses. The dominant microorganisms during spontaneous fermentation were S. cerevisiae, H. uvarum, H. guilliermondii, Lactobacillus fermentum, Pediococcus sp., and Acetobacter pasteurianus. Similarly, S. cerevisiae, P. kluyveri, Candida sp., Pediococcus sp., and A. pasteurianus were the predominant microorganisms assessed by Denaturing Gradient Gel Electrophoresis (DGGE) in inoculated fermentation. Sixty-seven volatile compounds were detected and quantified by gas chromatography/mass spectrometry (GC/MS) at the end of fermentation and chocolates. The main group of volatile compound found after the inoculated and spontaneous fermentations was esters (41 and 39 %, respectively). In the chocolates, the main group was acids (73 and 44 % from the inoculated and spontaneous fermentations, respectively). The TDS analyses showed a dominance of bitter and cocoa attributes in both chocolates. However, in the inoculated chocolate, lingering fruity notes were more intense, while the chocolate produced by spontaneous fermentation was more astringent. Thus, the inoculation of yeast influenced the microbial profile, which likely affected the volatile compounds that affect sensory characteristics, resulting in chocolate with dominant bitter, cocoa, and fruity attributes.

  12. Picasso on Show in Shanghai

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    A staff member of the National Picasso Museum of France checks one of the great Spanish artist Pablo Picasso’s works at the China Pavilion inside the site of the 2010 World Expo in Shanghai on October 12.Sixty-two priceless paintings and statues selected from the works of the renowned artist have been brought to the pavilion for an upcoming exhibition to premiere on October 18.Besides these representative masterpieces,50 valuable photographs showing the artist’s whole life will also be presented.The exhibition’s estimated value is 678 million euros ($934 million).It will be held until January 10,2012.

  13. Graphene Oxides Show Angiogenic Properties.

    Science.gov (United States)

    Mukherjee, Sudip; Sriram, Pavithra; Barui, Ayan Kumar; Nethi, Susheel Kumar; Veeriah, Vimal; Chatterjee, Suvro; Suresh, Kattimuttathu Ittara; Patra, Chitta Ranjan

    2015-08-05

    Angiogenesis, a process resulting in the formation of new capillaries from the pre-existing vasculature plays vital role for the development of therapeutic approaches for cancer, atherosclerosis, wound healing, and cardiovascular diseases. In this report, the synthesis, characterization, and angiogenic properties of graphene oxide (GO) and reduced graphene oxide (rGO) have been demonstrated, observed through several in vitro and in vivo angiogenesis assays. The results here demonstrate that the intracellular formation of reactive oxygen species and reactive nitrogen species as well as activation of phospho-eNOS and phospho-Akt might be the plausible mechanisms for GO and rGO induced angiogenesis. The results altogether suggest the possibilities for the development of alternative angiogenic therapeutic approach for the treatment of cardiovascular related diseases where angiogenesis plays a significant role.

  14. Phytoceramide Shows Neuroprotection and Ameliorates Scopolamine-Induced Memory Impairment

    Directory of Open Access Journals (Sweden)

    Seikwan Oh

    2011-10-01

    Full Text Available The function and the role phytoceramide (PCER and phytosphingosine (PSO in the central nervous system has not been well studied. This study was aimed at investigating the possible roles of PCER and PSO in glutamate-induced neurotoxicity in cultured neuronal cells and memory function in mice. Phytoceramide showed neuro-protective activity in the glutamate-induced toxicity in cultured cortical neuronal cells. Neither phytosphingosine nor tetraacetylphytosphingosine (TAPS showed neuroproective effects in neuronal cells. PCER (50 mg/kg, p.o. recovered the scopolamine-induced reduction in step-through latency in the passive avoidance test; however, PSO did not modulate memory function on this task. The ameliorating effects of PCER on spatial memory were confirmed by the Morris water maze test. In conclusion, through behavioral and neurochemical experimental results, it was demonstrated that central administration of PCER produces amelioration of memory impairment. These results suggest that PCER plays an important role in neuroprotection and memory enhancement and PCER could be a potential new therapeutic agent for the treatment of neurodegenerative diseases such as Alzheimer’s disease.

  15. Co-culture of neural crest stem cells (NCSC and insulin producing beta-TC6 cells results in cadherin junctions and protection against cytokine-induced beta-cell death.

    Directory of Open Access Journals (Sweden)

    Anongnad Ngamjariyawat

    Full Text Available PURPOSE: Transplantation of pancreatic islets to Type 1 diabetes patients is hampered by inflammatory reactions at the transplantation site leading to dysfunction and death of insulin producing beta-cells. Recently we have shown that co-transplantation of neural crest stem cells (NCSCs together with the islet cells improves transplantation outcome. The aim of the present investigation was to describe in vitro interactions between NCSCs and insulin producing beta-TC6 cells that may mediate protection against cytokine-induced beta-cell death. PROCEDURES: Beta-TC6 and NCSC cells were cultured either alone or together, and either with or without cell culture inserts. The cultures were then exposed to the pro-inflammatory cytokines IL-1β and IFN-γ for 48 hours followed by analysis of cell death rates (flow cytometry, nitrite production (Griess reagent, protein localization (immunofluorescence and protein phosphorylation (flow cytometry. RESULTS: We observed that beta-TC6 cells co-cultured with NCSCs were protected against cytokine-induced cell death, but not when separated by cell culture inserts. This occurred in parallel with (i augmented production of nitrite from beta-TC6 cells, indicating that increased cell survival allows a sustained production of nitric oxide; (ii NCSC-derived laminin production; (iii decreased phospho-FAK staining in beta-TC6 cell focal adhesions, and (iv decreased beta-TC6 cell phosphorylation of ERK(T202/Y204, FAK(Y397 and FAK(Y576. Furthermore, co-culture also resulted in cadherin and beta-catenin accumulations at the NCSC/beta-TC6 cell junctions. Finally, the gap junction inhibitor carbenoxolone did not affect cytokine-induced beta-cell death during co-culture with NCSCs. CONCLUSION: In summary, direct contacts, but not soluble factors, promote improved beta-TC6 viability when co-cultured with NCSCs. We hypothesize that cadherin junctions between NCSC and beta-TC6 cells promote powerful signals that maintain beta

  16. Reality shows: uma abordagem psicossocial

    Directory of Open Access Journals (Sweden)

    Marília Pereira Bueno Millan

    Full Text Available Desde os primórdios da civilização, o ser humano mostra necessidade de representar cenicamente seus dramas pessoais e vicissitudes existenciais. O "reality show" é uma das versões pós-modernas da encenação da vida humana. Este artigo, por meio de uma pesquisa bibliográfica, analisa criticamente as relações existentes entre o "reality show" e aspectos psicossociais do comportamento humano. Conclui-se que tais programas televisivos são o retrato da contemporaneidade, ou seja, revelam a morte do sujeito, a fugacidade das experiências vividas, a desvalorização da história e o culto à imagem e à superficialidade. Por meio da sedução do espectador, mobilizam-se aspectos primitivos de seu psiquismo, fazendo com que ele se sinta narcisicamente poderoso e onipotente e se acredite dono do destino dos participantes do programa. Sugerem-se novos estudos que contribuam para a reflexão crítica e maior conscientização.

  17. "Medicine show." Alice in Doctorland.

    Science.gov (United States)

    1987-01-01

    This is an excerpt from the script of a 1939 play provided to the Institute of Social Medicine and Community Health by the Library of Congress Federal Theater Project Collection at George Mason University Library, Fairfax, Virginia, pages 2-1-8 thru 2-1-14. The Federal Theatre Project (FTP) was part of the New Deal program for the arts 1935-1939. Funded by the Works Progress Administration (WPA) its goal was to employ theater professionals from the relief rolls. A number of FTP plays deal with aspects of medicine and public health. Pageants, puppet shows and documentary plays celebrated progress in medical science while examining social controversies in medical services and the public health movement. "Medicine Show" sharply contrasts technological wonders with social backwardness. The play was rehearsed by the FTP but never opened because funding ended. A revised version ran on Broadway in 1940. The preceding comments are adapted from an excellent, well-illustrated review of five of these plays by Barabara Melosh: "The New Deal's Federal Theatre Project," Medical Heritage, Vol. 2, No. 1 (Jan/Feb 1986), pp. 36-47.

  18. An assessment of cultural values and resident-centered culture change in U.S. nursing facilities.

    Science.gov (United States)

    Banaszak-Holl, Jane; Castle, Nicholas G; Lin, Michael; Spreitzer, Gretchen

    2013-01-01

    Culture change initiatives propose to improve care by addressing the lack of managerial supports and prevalent stressful work environments in the industry; however, little is known about how culture change facilities differ from facilities in the industry that have not chosen to affiliate with the resident-centered care movements. The aim of this study was to evaluate representation of organizational culture values within a random sample of U.S. nursing home facilities using the competing values framework and to determine whether organizational values are related to membership in resident-centered culture change initiatives. We collected reports of cultural values using a well-established competing values framework instrument in a random survey of facility administrators and directors of nursing within all states. We received responses from 57% of the facilities that were mailed the survey. Directors of nursing and administrators did not differ significantly in their reports of culture and facility measures combined their responses. Nursing facilities favored market-focused cultural values on average, and developmental values, key to innovation, were the least common across all nursing homes. Approximately 17% of the facilities reported that all cultural values were strong within their facilities. Only high developmental cultural values were linked to participation in culture change initiatives. Culture change facilities were not different from non-culture change facilities in the promotion of employee focus as organizational culture, as emphasized in group culture values. Likewise, culture change facilities were also not more likely to have hierarchical or market foci than non-culture change facilities. Our results counter the argument that culture change facilities have a stronger internal employee focus than facilities more generally but do show that culture change facilities report stronger developmental cultures than non-culture change facilities, which

  19. Talking Culture

    DEFF Research Database (Denmark)

    Holmgreen, Lise-Lotte

    When Danish businesses move production abroad, ‘culture’ is often seen as a huge challenge to the successful outcome of cross-border collaboration. Therefore, business leaders often seek information and guidelines of how to cope in the vast amount of literature on culture and intercultural...... communication. Much of this literature is based on functionalist approaches providing the dos and don’ts of intercultural encounters. This involves inter alia conceptualising ‘culture’ as a relatively fixed, homogeneous entity of values, attitudes and norms shared by members of a group, often leading readers...... to adopt dichotomised understandings and discourses about other cultures (see e.g. Hofstede 2001; Jandt 1998; Trompenaars & Hampden-Turner 1997). However, experience shows that the world in which intercultural encounters take place is not as simple and easy to categorise as these approaches may suggest...

  20. Casimir experiments showing saturation effects

    CERN Document Server

    Sernelius, Bo E

    2009-01-01

    We address several different Casimir experiments where theory and experiment disagree. First out is the classical Casimir force measurement between two metal half spaces; here both in the form of the torsion pendulum experiment by Lamoreaux and in the form of the Casimir pressure measurement between a gold sphere and a gold plate as performed by Decca et al.; theory predicts a large negative thermal correction, absent in the high precision experiments. The third experiment is the measurement of the Casimir force between a metal plate and a laser irradiated semiconductor membrane as performed by Chen et al.; the change in force with laser intensity is larger than predicted by theory. The fourth experiment is the measurement of the Casimir force between an atom and a wall in the form of the measurement by Obrecht et al. of the change in oscillation frequency of a 87 Rb Bose-Einstein condensate trapped to a fused silica wall; the change is smaller than predicted by theory. We show that saturation effects can exp...

  1. Enhancing students' cultural competence using cross-cultural experiential learning.

    Science.gov (United States)

    Kratzke, Cynthia; Bertolo, Melissa

    2013-01-01

    The purpose of this study was to explore undergraduate community health students' perceptions of their cultural competence. Little is known about students' cultural awareness, knowledge, and skills after their experience working with diverse cultural groups and language barriers. A cross-cultural experiential learning exercise was used as an educational approach. Reflective writing was used to elicit students' attitudes of the other culture and their coping skills. Three themes emerged as cultural awareness and knowledge, observation and learning, and cross-cultural communication. Results underscore the need for student academic preparation using cross-cultural educational approaches to enhance cultural competence.

  2. The potential probiotic Lactobacillus rhamnosus CTC1679 survives the passage through the gastrointestinal tract and its use as starter culture results in safe nutritionally enhanced fermented sausages.

    Science.gov (United States)

    Rubio, Raquel; Martín, Belén; Aymerich, Teresa; Garriga, Margarita

    2014-09-01

    The human-derived potential probiotic strain Lactobacillus rhamnosus CTC1679 was used as a starter culture in reduced fat and sodium low-acid fermented sausages (fuets) to assess its ability to survive through the gastrointestinal tract (GIT) in a human intervention study consisting of 5 healthy volunteers who consumed 25 g fuet a day for 21 days. Faecal samples were analysed during and after consumption. L. rhamnosus CTC1679 produced a transient colonisation of the human GIT and persisted during the ingestion period of fuet containing L. rhamnosus CTC1679 at levels ca. 8log CFU/g. After 3 days of non-consumption, the strain was still recovered in the faeces of all the volunteers. To evaluate the safety of the nutritionally enhanced manufactured fuets, a challenge test was designed in a separately manufactured batch. L. rhamnosus CTC1679 was able to grow, survive and dominate (levels ca. 10(8) CFU/g) the endogenous lactic acid bacteria (LAB), prevented the growth of Listeria monocytogenes throughout the whole ripening process of the fuets and eliminated Salmonella. After 35 days of storage at 4 °C, L. monocytogenes was not detected, achieving absence in 25 g of the product. The application of high hydrostatic pressure (HHP) treatment (600 MPa for 5 min) at the end of ripening (day 14) produced an immediate reduction of L. monocytogenes to levels fermented sausages.

  3. Do the Associations of Parenting Styles With Behavior Problems and Academic Achievement Vary by Culture? Results From a Meta-Analysis.

    Science.gov (United States)

    Pinquart, Martin; Kauser, Rubina

    2017-04-10

    The study tested whether associations of parenting styles with internalizing problems, externalizing problems, and academic achievement vary between ethnic groups in western countries, between different regions of the globe, and by level of collectivism/individualism of individual countries. A systematic search in electronic databases and cross referencing identified 428 studies that were included in the random-effects meta-analysis. More ethnic and regional similarities than differences were identified. In western countries, associations of authoritative parenting with academic achievement were stronger in non-Hispanic, White families than in Asian minorities. In these countries, associations of authoritarian parenting with academic achievement were less negative in Hispanic families than in non-Hispanic, White families. Authoritative parenting was associated with at least 1 positive child outcome and authoritarian parenting was associated with at least 1 negative outcome in all regions of the globe, with some regional variation. Finally, associations of authoritarian parenting with child outcomes were weaker in countries with a higher individualism score, as were associations of authoritative parenting with academic performance. Parents across the globe could be recommended to behave authoritatively, although authoritarian and permissive parenting is, to some extent, tolerable in a few cultural contexts. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  4. Examining cultural drifts in artworks through history and development: cultural comparisons between Japanese and western landscape paintings and drawings.

    Science.gov (United States)

    Nand, Kristina; Masuda, Takahiko; Senzaki, Sawa; Ishii, Keiko

    2014-01-01

    Research on cultural products suggest that there are substantial cultural variations between East Asian and European landscape masterpieces and contemporary members' landscape artwork (Masuda et al., 2008c), and that these cultural differences in drawing styles emerge around the age of 8 (Senzaki et al., 2014b). However, culture is not static. To explore the dynamics of historical and ontogenetic influence on artistic expressions, we examined (1) 17-20th century Japanese and Western landscape masterpieces, and (2) cross-sectional adolescent data in landscape artworks alongside previous findings of elementary school-aged children, and undergraduates. The results showed cultural variations in artworks and masterpieces as well as substantial "cultural drifts" (Herskovits, 1948) where at certain time periods in history and in development, people's expressions deviated from culturally default patterns but occasionally returned to its previous state. The bidirectional influence of culture and implications for furthering the discipline of cultural psychology will be discussed.

  5. Examining cultural drifts in artworks through history and development: cultural comparisons between Japanese and western landscape paintings and drawings

    Science.gov (United States)

    Nand, Kristina; Masuda, Takahiko; Senzaki, Sawa; Ishii, Keiko

    2014-01-01

    Research on cultural products suggest that there are substantial cultural variations between East Asian and European landscape masterpieces and contemporary members' landscape artwork (Masuda et al., 2008c), and that these cultural differences in drawing styles emerge around the age of 8 (Senzaki et al., 2014b). However, culture is not static. To explore the dynamics of historical and ontogenetic influence on artistic expressions, we examined (1) 17–20th century Japanese and Western landscape masterpieces, and (2) cross-sectional adolescent data in landscape artworks alongside previous findings of elementary school-aged children, and undergraduates. The results showed cultural variations in artworks and masterpieces as well as substantial “cultural drifts” (Herskovits, 1948) where at certain time periods in history and in development, people's expressions deviated from culturally default patterns but occasionally returned to its previous state. The bidirectional influence of culture and implications for furthering the discipline of cultural psychology will be discussed. PMID:25285085

  6. Examining Cultural Drifts in Artworks through History and Development: Cultural Comparisons between Japanese and Western Landscape Paintings and Drawings.

    Directory of Open Access Journals (Sweden)

    Kristina eNand

    2014-09-01

    Full Text Available Research on cultural products suggest that there are substantial cultural variations between East Asian and European landscape masterpieces and contemporary members’ landscape artwork (Masuda et al., 2008, and that these cultural differences in drawing styles emerge around the age of 8 (Senzaki et al., 2014. However, culture is not static. To explore the dynamics of historical and ontogenetic influence on artistic expressions, we examined (1 17th to 20th century Japanese and Western landscape masterpieces, and (2 cross-sectional adolescent data in landscape artworks alongside previous findings of elementary school-aged children, and undergraduates. The results showed cultural variations in artworks and masterpieces as well as substantial cultural drifts (Herskovits, 1948 where at certain time periods in history and in development, people’s expressions deviated from culturally default patterns but occasionally returned to its previous state. The bidirectional influence of culture and implications for furthering the discipline of cultural psychology will be discussed.

  7. Cross cultural usability testing

    DEFF Research Database (Denmark)

    Clemmensen, Torkil; Goyal, Shivam

    2005-01-01

    In this paper, we present the results of a pilot study in Denmark of cross cultural effects on Think Aloud usability testing. We provide an overview of previous research on cross cultural usability evaluation with a special focus on the relationship between the evaluator and the test user....... This relation was studied in an experiment with usability testing of a localized clipart application in which eight participants from Denmark and India formed pairs of evaluator-test user. The test users were asked to think aloud and the evaluators' role were to facilitate the test users thinking aloud...... and hereby identify usability problems with the clipart application. Data on the evaluators' and test users' behaviour were recorded and analyzed by coding and summarizing statistics on these behavioural events. The results show that Think Aloud Usability Test of a localized application is most effectively...

  8. THE INTEGRATION OF CULTURAL RESOURCES MANAGEMENT IN DISASTER MANAGEMENT AT SPECIAL REGION PROVINCE OF YOGYAKARTA

    Directory of Open Access Journals (Sweden)

    Deffi Ayu Puspito Sari

    2017-03-01

    Full Text Available The Republic of Indonesia Law number 24 year 2007 on disaster emphasizes that the protection of national assets is in line with Law No. 11 year 2010 on the cultural heritage. Yogyakarta Province has 12 disaster hazards and has five complete archaeological cultural layers in Indonesia. In the event of a disaster, potential damage to the cultural heritage is exposed. The archaeological cultural layer consists of prehistoric, classical, Islamic and colonial. The lack of research related to cultural heritage in the province resulting in increasing vulnerability of cultural heritage and society. Using qualitative method with in-depth interview, the aim of this study is to analyse the management of cultural heritage from the perspective of disaster management. Archaeological cultural layers that embedded into the realm of cultural heritage is defined as a national asset that should be protected. The result shows that the management of cultural resources in the province is not yet integrated with disaster management. However, the results of the archaeological identification of cultural heritage in each cultural layer in Yogyakarta showed the development of community adaptation to the disaster. Utilization of cultural heritage as an element of the panca gatra has been impartial that affected the regional resilience and security in facing the disaster. Both of these problems can be overcome by integrating the cultural resources management and disaster management, the establishment of an emergency response team on cultural preservation, and disaster risk analysis on cultural heritage that annexed by BPBD and Cultural Office of Yogyakarta Province.

  9. Culture and social class.

    Science.gov (United States)

    Miyamoto, Yuri

    2017-08-08

    A large body of research in Western cultures has demonstrated the psychological and health effects of social class. This review outlines a cultural psychological approach to social stratification by comparing psychological and health manifestations of social class across Western and East Asian cultures. These comparisons suggest that cultural meaning systems shape how people make meaning and respond to material/structural conditions associated with social class, thereby leading to culturally divergent manifestations of social class. Specifically, unlike their counterparts in Western cultures, individuals of high social class in East Asian cultures tend to show high conformity and other-orientated psychological attributes. In addition, cultures differ in how social class impacts health (i.e. on which bases, through which pathways, and to what extent). Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Clinical Analysis of a New Hospital Patients with Blood Culture Results in Department of Respiration Pneumonia%新建医院呼吸科肺炎住院患者血培养结果临床分析

    Institute of Scientific and Technical Information of China (English)

    成苏杭

    2014-01-01

    [ABSTRACT]Objective: A preliminary analysis of blood culture positive rate in pneumonia patients be in new hospital. Methods:Get a selection of 81 cases of pneumonia in hospital patients,with reference to the ministry of health diagnosis and treatment guidelines and the clinical pathway for pneumonia,and 38 patients with blood culture were match the inspection condition, analysis the blood culture and influence factors were positive. Result: Only 1 report was positive in 38 cases of blood culture in pneumonia patients. Conclusion:We should pay attention to the examine of pathogeny in pneumonia patients.%目的:初步分析新建医院肺炎住院患者血培养阳性率。方法:参照卫生部的肺炎诊疗指南及临床路径,选取81例肺炎住院患者,其中38例患者符合血培养检查条件,分析血培养阳性率结果及影响因素。结果:38例肺炎住院患者行血培养检查,结果只有1例血培养报告阳性。结论:新建医院需重视肺炎患者病原学检查。

  11. Urine culture

    Science.gov (United States)

    Culture and sensitivity - urine ... when urinating. You also may have a urine culture after you have been treated for an infection. ... when bacteria or yeast are found in the culture. This likely means that you have a urinary ...

  12. Splitting of IVP bovine blastocyst affects morphology and gene expression of resulting demi-embryos during in vitro culture and in vivo elongation.

    Science.gov (United States)

    Velasquez, Alejandra E; Castro, Fidel O; Veraguas, Daniel; Cox, Jose F; Lara, Evelyn; Briones, Mario; Rodriguez-Alvarez, Lleretny

    2016-02-01

    Embryo splitting might be used to increase offspring yield and for molecular analysis of embryo competence. How splitting affects developmental potential of embryos is unknown. This research aimed to study the effect of bovine blastocyst splitting on morphological and gene expression homogeneity of demi-embryos and on embryo competence during elongation. Grade I bovine blastocyst produced in vitro were split into halves and distributed in nine groups (3 × 3 setting according to age and stage before splitting; age: days 7-9; stage: early, expanded and hatched blastocysts). Homogeneity and survival rate in vitro after splitting (12 h, days 10 and 13) and the effect of splitting on embryo development at elongation after embryo transfer (day 17) were assessed morphologically and by RT-qPCR. The genes analysed were OCT4, SOX2, NANOG, CDX2, TP1, TKDP1, EOMES, and BAX. Approximately 90% of split embryos had a well conserved defined inner cell mass (ICM), 70% of the halves had similar size with no differences in gene expression 12 h after splitting. Split embryos cultured further conserved normal and comparable morphology at day 10 of development; this situation changes at day 13 when embryo morphology and gene expression differed markedly among demi-embryos. Split and non-split blastocysts were transferred to recipient cows and were recovered at day 17. Fifty per cent of non-split embryos were larger than 100 mm (33% for split embryos). OCT4, SOX2, TP1 and EOMES levels were down-regulated in elongated embryos derived from split blastocysts. In conclusion, splitting day-8 blastocysts yields homogenous demi-embryos in terms of developmental capability and gene expression, but the initiation of the filamentous stage seems to be affected by the splitting.

  13. Safeguards Culture

    Energy Technology Data Exchange (ETDEWEB)

    Frazar, Sarah L.; Mladineo, Stephen V.

    2012-07-01

    The concepts of nuclear safety and security culture are well established; however, a common understanding of safeguards culture is not internationally recognized. Supported by the National Nuclear Security Administration, the authors prepared this report, an analysis of the concept of safeguards culture, and gauged its value to the safeguards community. The authors explored distinctions between safeguards culture, safeguards compliance, and safeguards performance, and evaluated synergies and differences between safeguards culture and safety/security culture. The report concludes with suggested next steps.

  14. NBA扣篮展示的篮球文化对篮球训练起到的导向作用%NBA's Dunking Show Basketball Culture and its Guiding Role to the Improvement of China's Basketball Training

    Institute of Scientific and Technical Information of China (English)

    李向前

    2011-01-01

    NBA篮球技术层文化是以篮球核心技术——投篮为载体的,而扣篮这一独特、富有激情与想象的技术,所展现的是美国篮球的个性文化、球星文化、审美文化。NBA扣篮展示文化符合了现阶段篮球运动发展的特征、规律,引领着美国篮球乃至世界篮球的发展方向,对我国篮球训练水平的提高起着重要的导向作用。%Basketball is an upward movement.All the movements of basketball is finally for upward.NBA’s technology culture is based on basketball’s core technology — shooting,which can reflect American basketball culture such as: personality culture,star culture,aesthetic culture.NBA’s "dunking" culture accords with characteristics and laws of present basketball development,and leads the directions of American basketball development and even world basketball development.Therefore it plays an important guiding role to the improvement of China’s basketball.

  15. Organizational Culture

    Directory of Open Access Journals (Sweden)

    Adrian HUDREA

    2006-02-01

    Full Text Available Cultural orientations of an organization can be its greatest strength, providing the basis for problem solving, cooperation, and communication. Culture, however, can also inhibit needed changes. Cultural changes typically happen slowly – but without cultural change, many other organizational changes are doomed to fail. The dominant culture of an organization is a major contributor to its success. But, of course, no organizational culture is purely one type or another. And the existence of secondary cultures can provide the basis for change. Therefore, organizations need to understand the cultural environments and values.

  16. Light and temperature effects on δ11B and B / Ca ratios of the zooxanthellate coral Acropora sp.: results from culturing experiments

    Directory of Open Access Journals (Sweden)

    P. Louvat

    2012-11-01

    Full Text Available The boron isotopic composition (δ11B of marine carbonates (e.g. corals is increasingly utilised as a proxy for paleo-pH, with the strong correlation between δ11B of marine calcifiers and seawater pH now well documented. However, the potential roles of other environmental parameters that may also influence both the boron isotopic composition and boron concentration into coral aragonite are poorly known. To overcome this, the tropical scleractinian coral Acropora sp. was cultured under 3 different temperatures (22, 25 and 28 °C and two light conditions (200 and 400 μmol photon m−2 s−1. The δ11B indicates an increase in internal pH that is dependent on the light conditions. Changes in light intensities from 200 to 400 μmol photon m−2 s−1 seem to indicate an apparent decrease in pH at the site of calcification, contrary to what is expected in most models of light-enhanced calcification. Thus, variations in light conditions chosen to mimic average annual variations of the natural environments where Acropora sp. colonies can be found could bias pH reconstructions by about 0.05 units. For both light conditions, a significant impact of temperature on δ11B can be observed between 22 and 25 °C, corresponding to an increase of about 0.02 pH-units, while no further δ11B increase can be observed from 25 to 28 °C. This non-linear temperature effect complicates the determination of a correction factor. B / Ca ratios decrease with increasing light, consistent with the decrease in pH at the site of calcification under enhanced light intensities. When all the other parameters are constant, boron concentrations in Acropora sp. increase with increasing temperatures and increasing carbonate ion concentrations. These observations contradict previous studies where B / Ca in corals was found to vary inversely with temperature, suggesting that the controlling factors driving boron concentrations have not yet been adequately identified and might be

  17. 利用抗氧化剂促进羊水细胞培养效果的研究%The study of improving the culture results of amniotic fluid cells by adding antioxidants

    Institute of Scientific and Technical Information of China (English)

    丘力功; 蔡小杰; 黄志诚

    2012-01-01

    目的 通过添加抗氧化剂促进羊水细胞的培养效果.方法 分离传代的成纤维型羊水细胞,以生长指数为检测指标,在H配方的基础上利用部分析因设计法和最陡爬坡法对促进羊水细胞生长的抗氧化剂进行筛选和优化.优化后的培养基通过原代羊水细胞培养进行效果验证.结果 添加的维生素E、L-抗坏血酸、α硫辛酸和还原型谷胱甘肽等抗氧化剂皆能促进羊水细胞的生长,且具有协同效应.与H培养基相比,经最陡爬坡法优化的培养基培养的羊水细胞在克隆总数、生长指数等指标都提高3倍以上,分裂相细胞总数增长4倍以上.结论 改良后的羊水细胞培养基培养效果要显著优于H配方培养基,在产前诊断应用中具有推广价值.%Objective To improve the culture results of amniotic fluid by adding antioxidants. Methods Using Fractional factorial designs combined with the steepest ascent path approach, the concentration of antioxidants added in H medium was optimized to increase the growth speed of the passage fibroblast cells from amniotic fluid . And the performance of the optimized culture medium was tested with the growth index, the numbers of clones and metaphase of primary amniotic fluid cells culture. Results Vitamin E, L-ascorbic acid,α-lipoic acid and reduced CSH can all promote the growth of the amniotic fluid cells with synergic effect. Total number of clones,growth index,and total number of metaphase cells from amniotic fluid cells cultured in medium optimized by the steepest ascent path approach are all 3 times higher than those from H medium. Conclusion The optimization method of adding antioxidants in H medium can improve significantly the results of amniotic fluid cells culture and the optimized culture medium is applicable to the prenatal diagnosis.

  18. Gene–culture coevolution in whales and dolphins

    Science.gov (United States)

    Whitehead, Hal

    2017-01-01

    Whales and dolphins (Cetacea) have excellent social learning skills as well as a long and strong mother–calf bond. These features produce stable cultures, and, in some species, sympatric groups with different cultures. There is evidence and speculation that this cultural transmission of behavior has affected gene distributions. Culture seems to have driven killer whales into distinct ecotypes, which may be incipient species or subspecies. There are ecotype-specific signals of selection in functional genes that correspond to cultural foraging behavior and habitat use by the different ecotypes. The five species of whale with matrilineal social systems have remarkably low diversity of mtDNA. Cultural hitchhiking, the transmission of functionally neutral genes in parallel with selective cultural traits, is a plausible hypothesis for this low diversity, especially in sperm whales. In killer whales the ecotype divisions, together with founding bottlenecks, selection, and cultural hitchhiking, likely explain the low mtDNA diversity. Several cetacean species show habitat-specific distributions of mtDNA haplotypes, probably the result of mother–offspring cultural transmission of migration routes or destinations. In bottlenose dolphins, remarkable small-scale differences in haplotype distribution result from maternal cultural transmission of foraging methods, and large-scale redistributions of sperm whale cultural clans in the Pacific have likely changed mitochondrial genetic geography. With the acceleration of genomics new results should come fast, but understanding gene–culture coevolution will be hampered by the measured pace of research on the socio-cultural side of cetacean biology. PMID:28739936

  19. Gene-culture coevolution in whales and dolphins.

    Science.gov (United States)

    Whitehead, Hal

    2017-07-24

    Whales and dolphins (Cetacea) have excellent social learning skills as well as a long and strong mother-calf bond. These features produce stable cultures, and, in some species, sympatric groups with different cultures. There is evidence and speculation that this cultural transmission of behavior has affected gene distributions. Culture seems to have driven killer whales into distinct ecotypes, which may be incipient species or subspecies. There are ecotype-specific signals of selection in functional genes that correspond to cultural foraging behavior and habitat use by the different ecotypes. The five species of whale with matrilineal social systems have remarkably low diversity of mtDNA. Cultural hitchhiking, the transmission of functionally neutral genes in parallel with selective cultural traits, is a plausible hypothesis for this low diversity, especially in sperm whales. In killer whales the ecotype divisions, together with founding bottlenecks, selection, and cultural hitchhiking, likely explain the low mtDNA diversity. Several cetacean species show habitat-specific distributions of mtDNA haplotypes, probably the result of mother-offspring cultural transmission of migration routes or destinations. In bottlenose dolphins, remarkable small-scale differences in haplotype distribution result from maternal cultural transmission of foraging methods, and large-scale redistributions of sperm whale cultural clans in the Pacific have likely changed mitochondrial genetic geography. With the acceleration of genomics new results should come fast, but understanding gene-culture coevolution will be hampered by the measured pace of research on the socio-cultural side of cetacean biology.

  20. How Cultural Knowledge Shapes Design Thinking

    DEFF Research Database (Denmark)

    Clemmensen, Torkil; Ranjan, Apara; Bødker, Mads

    2016-01-01

    This paper challenges the ‘core design thinking and its application’ as outlined by Dorst (2011) and uses a dynamic constructivist notion of cultural-cognitive performance to analyze aspects of a design thinking process (Clemmensen, 2009; Hong & Mallorie, 2004). Based on a qualitative analysis...... of some of the events in the DTRS11 data set and using the theory of Dorst on design thinking as well as Hong & Mallorie’s socio-cognitive theory of cultural knowledge networks, the paper shows how it is possible and useful to analyze design thinking from a cultural perspective. The results show...... that cultural knowledge, either as shared knowledge by the cross-cultural team or group specific knowledge, influences the Dorst design thinking equations across all the 16 episodes analyzed in DTRS11 data set. Furthermore, most of the design discussions were approached by the designers as problem situations...

  1. How Cultural Knowledge Shapes Design Thinking

    DEFF Research Database (Denmark)

    Clemmensen, Torkil; Ranjan, Apara; Bødker, Mads

    2016-01-01

    This paper challenges the ‘core design thinking and its application’ as outlined by Dorst (2011) and uses a dynamic constructivist notion of cultural-cognitive performance to analyze aspects of a design thinking process (Clemmensen, 2009; Hong & Mallorie, 2004). Based on a qualitative analysis...... of some of the events in the DTRS11 data set and using the theory of Dorst on design thinking as well as Hong & Mallorie’s socio-cognitive theory of cultural knowledge networks, the paper shows how it is possible and useful to analyze design thinking from a cultural perspective. The results show...... that cultural knowledge, either as shared knowledge by the cross-cultural team or group specific knowledge, influences the Dorst design thinking equations across all the 16 episodes analyzed in DTRS11 data set. Furthermore, most of the design discussions were approached by the designers as problem situations...

  2. The Empirical Research on the Results of Cultural Teaching in the Perspective of Listening and Audio-visual Reform%大学英语视听说教改视域下的文化教学研究

    Institute of Scientific and Technical Information of China (English)

    王一鸣

    2014-01-01

    无论是培养学生的实际运用能力,还是增进学生跨文化交际素质,视听说作为培养学生口语交际能力的一门课程,都具有重要的作用;该课题旨在研究文化教学在视听说课程改革中的教学效果,通过与无文化教学的传统视听说教学方式对比,研究发现融合文化因素的教学方式对学生的口语能力有更大的促进作用,促进作用主要体现在学生口语交际的流利性,准确度和地道性三个方面。%English Listening and speaking course has always been playing a significant role in cultivating students' cross cultural communicative competence as well as practical communication. This paper tends to research on the impacts of cultural teaching compared with the traditional audio-visual teaching method, which shows that the teaching method integrated with cultural fac-tors is prone to have even greater impacts on students oral English and tend to make their speaking more fluent, much more accu-rate and more idiomatic.

  3. Industrial cultures

    DEFF Research Database (Denmark)

    Rasmussen, Lauge Baungaard

    1996-01-01

    The chapter deals with different paradigms andtheories of cultural development. The problem toexplain change and methods to analyse developmentin different cultures are presented and discussed.......The chapter deals with different paradigms andtheories of cultural development. The problem toexplain change and methods to analyse developmentin different cultures are presented and discussed....

  4. CORE CULTURE IN LANGUAGE LEARNING

    Institute of Scientific and Technical Information of China (English)

    Jane Orton

    2002-01-01

    This paper presents the essential role of culture in the creation of meaning of language-inuse, showing it to be the source of both the contextual shaping of a particular text and the fundamental core of beliefs and values from which contemporary contextual features derive. As a result of this essential relationship, it is argued, modern language learners need to master the cultural system as well as the linguistic system of their new language if they are to be able to use it competently in real life, as mo st intend today. Samples of texts are examined to show how the meanings of both context and core culture are naturally embedded in ordinary language and suggestions are provided for how these might be successfully brought to students' attention and mastery in a classroom.

  5. Cultural evolution and individual development of openness and conservatism.

    Science.gov (United States)

    Acerbi, Alberto; Enquist, Magnus; Ghirlanda, Stefano

    2009-11-10

    We present a model of cultural evolution in which an individual's propensity to engage in social learning is affected by social learning itself. We assume that individuals observe cultural traits displayed by others and decide whether to copy them based on their overall preference for the displayed traits. Preferences, too, can be transmitted between individuals. Our results show that such cultural dynamics tends to produce conservative individuals, i.e., individuals who are reluctant to copy new traits. Openness to new information, however, can be maintained when individuals need significant time to acquire the cultural traits that make them effective cultural models. We show that a gradual enculturation of young individuals by many models and a larger cultural repertoire to be acquired are favorable circumstances for the long-term maintenance of openness in individuals and groups. Our results agree with data about lifetime personality change, showing that openness to new information decreases with age. Our results show that cultural remodeling of cultural transmission is a powerful force in cultural evolution, i.e., that cultural evolution can change its own dynamics.

  6. MERICAN CULTURAL TOLERANCE

    Institute of Scientific and Technical Information of China (English)

    ZHANG RUIXIAO

    2014-01-01

    As an emigrant country, the essential characteristic of America culture is its tolerance. It contributes to the formation the diversity of American culture. By tracing back to American history, this essay shows what caused American cultural tolerance. Through describing briefly the manifestation of American cultural tolerance from certain aspects and analyzing the major factors, it will give us a clue about the reason why America can be always prosperous. At last, the paper shows the limitation as well as advantages about the tolerance from the point of current status.

  7. Biological Control of Aphid Using Fungal Culture and Culture Filtrates of Beauveria bassiana.

    Science.gov (United States)

    Kim, Jeong Jun; Jeong, Gayoung; Han, Ji Hee; Lee, Sangyeob

    2013-12-01

    Aphids are one of the most destructive pests in crop production such as pepper, cucumber, and eggplants. The importance of entomopathogenic fungi as alternative pest control agents is increasing. Conidia of entomopathogenic fungi are influenced by environmental conditions, such as temperature and relative humidity, and cause slow and fluctuating mortality. These factors have prevented wider application and use of biocontrol agents. For investigation of means of mitigation of such problems, we conducted bioassays with 47 fungal culture filtrates in order to evaluate the potential of secondary metabolites produced by entomopathogenic fungi for use in aphid control. Among 47 culture filtrates cultured potato dextrose broth, filtrate of Beauveria bassiana Bb08 showed the highest mortality (78%) against green peach aphid three days after treatments. Filtrate of Bb08 cultured in Adamek's medium showed higher toxicity as 100% to third instar nymphs of the aphid compared with seven other filtrates cultured in different broths amended with colloidal chitin or oil. The culture filtrates and fungal cultures from media amended with colloidal chitin or oil had lower control efficacies than filtrates without these additives in three different media. These results indicate that the fungal culture fluid or culture filtrate of B. bassiana Bb08 cultured in Adamek's medium has potential for development as a mycopesticide for aphid control.

  8. Pediatric blood culture: time to positivity.

    Science.gov (United States)

    Kara, Ateş; Kanra, Güler; Cengiz, A Bülent; Apiş, Menekşe; Gür, Deniz

    2004-01-01

    The aim of this study was to determine how long it takes blood culture to become positive using a blood culture system that can be monitored continuously in pediatric patients. Data were collected prospectively on 1,000 positive blood culture results from a tertiary pediatric university hospital from April 2000 to May 2002. The laboratory used the BACTEC 9120 fluorescent blood culture system. Patient's age ranged from less than a day to 20 years of age (mean 3 years). Five hundred and four cultures (50.4%) out of 1,000 yielded coagulase negative staphylococcus (CNS), 81 (8.1%) S. aureus, 53 (5.3%). Pseudomonas and 50 (5.0%) Klebsiella species. Of the 504 coagulase negative staphylococcal blood culture isolates, 314 (62.3% of CNS) were regarded as skin contaminants. Of the 1,000 cultures, 9.6% were reported as positive in the first day, 27.8% in the second day, 54.7% in the third day, 77.0% in the fourth and 89.4% in the fifth day. There was no association between previous antibiotic usage and the period required for isolate recovery. The clinician can expect to get results of positive blood cultures with susceptibility data, at a rate of 77.1% by day four and almost 90% by day five of sampling in the bacteriemic patient. Blood cultures yielding coagulase negative staphylococci in the first three days almost always show bacteremia with those microorganisms.

  9. Cultural effects on mindreading.

    Science.gov (United States)

    Perez-Zapata, Daniel; Slaughter, Virginia; Henry, Julie D

    2016-01-01

    People from other cultural backgrounds sometimes seem inscrutable. We identified a potential cause of this phenomenon in two experiments demonstrating that adults' mental state inferences are influenced by the cultural identity of the target. We adapted White, Hill, Happé, and Frith's (2009) Strange Stories to create matched intra-cultural and cross-cultural mindreading and control conditions. Experiment 1 showed that Australian participants were faster to respond and received higher scores in the intra-cultural mindreading condition relative to the cross-cultural mindreading condition, but performance in the control conditions was equivalent. Experiment 2 replicated this pattern in independent samples of Australian and Chilean participants. These findings have important implications for cross-cultural communication and understanding.

  10. THE IMPACT OF TRANSLATION WORKS AS AN ENCOUNTER AND INTERACTION TOOL OF CULTURES ON TURKISH CULTURE

    Directory of Open Access Journals (Sweden)

    Aliye USLU ÜSTTEN

    2016-12-01

    Full Text Available Culture is not only a set of beliefs and behaviors that separates a nation from others, but it is also a mirror which shows identity of the community. This common belief, feeling and thinking style is also reflected in the nation's fine arts and literature. Literature, by transferring culture to future generations through language, helps communication and realization of social integration. In line with the changes in society, the way of living and expectations of the human-being, which is the main subject of literature and the way which culture is handled change. National language creates a national culture; and national culture results in the concept of the national state. Therefore, stronger the bonds between the communities and their history and culture, richer and more closed to negative effects are the elements that feed the living culture. The changes that took place in the social structure of the Ottoman state in 19th century led to new developments in administration, economics, education as well as in the fields of culture and literature. Efforts towards modernization, which began with the concept of Westernization, put the literary scholars at a crossroads between the eastern customs and the translations made from the West. Translated works were seen as a bridge between Turkish society and the advanced countries in terms of culture and civilization. However, changes in cultural values such as customs and traditions, sense of humor, morals, also created negative impacts on Turkish culture. This study, which tries to demonstrate changes in social and cultural values of the Turkish society as a result of these influences, is based on translated texts starting from Tanzimat Period. We report the synthesis of cultural elements of the translated works with elements of Turkish culture.

  11. Measuring patient safety culture in Taiwan using the Hospital Survey on Patient Safety Culture (HSOPSC

    Directory of Open Access Journals (Sweden)

    Chen I-Chi

    2010-06-01

    Full Text Available Abstract Background Patient safety is a critical component to the quality of health care. As health care organizations endeavour to improve their quality of care, there is a growing recognition of the importance of establishing a culture of patient safety. In this research, the authors use the Hospital Survey on Patient Safety Culture (HSOPSC questionnaire to assess the culture of patient safety in Taiwan and attempt to provide an explanation for some of the phenomena that are unique in Taiwan. Methods The authors used HSOPSC to measure the 12 dimensions of the patient safety culture from 42 hospitals in Taiwan. The survey received 788 respondents including physicians, nurses, and non-clinical staff. This study used SPSS 15.0 for Windows and Amos 7 software tools to perform the statistical analysis on the survey data, including descriptive statistics and confirmatory factor analysis of the structural equation model. Results The overall average positive response rate for the 12 patient safety culture dimensions of the HSOPSC survey was 64%, slightly higher than the average positive response rate for the AHRQ data (61%. The results showed that hospital staff in Taiwan feel positively toward patient safety culture in their organization. The dimension that received the highest positive response rate was "Teamwork within units", similar to the results reported in the US. The dimension with the lowest percentage of positive responses was "Staffing". Statistical analysis showed discrepancies between Taiwan and the US in three dimensions, including "Feedback and communication about error", "Communication openness", and "Frequency of event reporting". Conclusions The HSOPSC measurement provides evidence for assessing patient safety culture in Taiwan. The results show that in general, hospital staffs in Taiwan feel positively toward patient safety culture within their organization. The existence of discrepancies between the US data and the Taiwanese data

  12. Cultural heritage training in the US military.

    Science.gov (United States)

    Svec, Leedjia

    2014-01-01

    Cultural competence is a vital component of many missions in today's military. Cultural competence enables one to further a mission, save resources, and save lives. Conversely, a lack of cultural competence may bring about challenges to mission completion, requirement for more resources, waste of resources, and destruction of lives. Cultural competence involves many components. One particular component is cultural heritage awareness and protection of cultural property. This study sought to assess current understanding of cultural property protection and determine the effectiveness of a training aimed at increasing cultural property protection awareness, knowledge, and comfort within the military setting. It was hypothesized that participants would vary in their level of awareness, knowledge, and comfort of cultural property protection, and that all would show a significant improvement in knowledge scores post training. Factors such as deployment experience were examined for potential correlation with measures such as awareness. A 14 question pre-read survey was developed to assess participants' demographics, awareness, knowledge, and comfort with cultural property protection. Awareness included value, laws, and procedures while knowledge examined "know how" such as how to bed down in a protected structure or communicate information about the structure. Comfort assessed one's comfort with engaging in the knowledge based tasks. A 24 question post read survey was administered to assess awareness, knowledge, and comfort, and to solicit additional feedback on the manual itself. The survey utilized a 1-5 rating scale with 1 representing no awareness, knowledge, or comfort and 5 representing absolute awareness, knowledge, and comfort with different aspects of cultural property protection. Cultural property protection value was highest pre and post training while knowledge regarding recovery of property was rated lowest pre and post training. Results are encouraging for

  13. Cultural Capital in Context:

    DEFF Research Database (Denmark)

    Andersen, Ida Gran; Jæger, Mads Meier

    This paper analyzes the extent to which the effect of cultural capital on academic achievement varies across high- and low-achieving schooling environments. We distinguish three competing theoretical models: Cultural reproduction (cultural capital yields higher returns in high-achieving schooling...... to be higher in low-achieving schooling environments than in high-achieving ones. These results support the cultural mobility explanation and are in line with previous research suggesting that children from low-SES families benefit more from cultural capital than children from high-SES families....

  14. Molecular Characterization of a Dechlorinating Community Resulting from In Situ Biostimulation in a Trichloroethene-Contaminated Deep, Fractured Basalt Aquifer and Comparison to a Derivative Laboratory Culture

    OpenAIRE

    Macbeth, Tamzen W.; Cummings, David E; Spring, Stefan; Petzke, Lynn M.; Sorenson, Kent S.

    2004-01-01

    Sodium lactate additions to a trichloroethene (TCE) residual source area in deep, fractured basalt at a U.S. Department of Energy site have resulted in the enrichment of the indigenous microbial community, the complete dechlorination of nearly all aqueous-phase TCE to ethene, and the continued depletion of the residual source since 1999. The bacterial and archaeal consortia in groundwater obtained from the residual source were assessed by using PCR-amplified 16S rRNA genes. A clone library of...

  15. The Value of Design and Development of the Modern Tourism and Cultural Goods Showing the Han and Tang Dynasty Style Characteristic%探析设计开发彰显汉唐风格特点的现代旅游文化商品价值

    Institute of Scientific and Technical Information of China (English)

    王欢

    2012-01-01

    Xi'an is a city with culture history, and its tourism resources is rich, but tourism commodity development has not reflected the level to match with history culture. The mutual relationship between culture and tourism industry has been confirmed, and also has been supported by policy, but related research have not been integrated. If we want to play the larger economic benefits of tourism culture commodity as the core of tourism products, related research needs supplementary.%西安是个文化历史名城,其旅游资源十分丰富,但在旅游商品开发上并没有体现出与其历史文化相匹配的水准,文化与旅游产业的相互关系已经得到证实,政策上也对其进行了扶持,但相关研究都没有在此得到整合,要想使旅游文化商品作为旅游产品的核心发挥更大的经济效益,相关领域的研究需要进行补充.

  16. Worldwide genetic and cultural change in human evolution.

    Science.gov (United States)

    Creanza, Nicole; Feldman, Marcus W

    2016-12-01

    Both genetic variation and certain culturally transmitted phenotypes show geographic signatures of human demographic history. As a result of the human cultural predisposition to migrate to new areas, humans have adapted to a large number of different environments. Migration to new environments alters genetic selection pressures, and comparative genetic studies have pinpointed numerous likely targets of this selection. However, humans also exhibit many cultural adaptations to new environments, such as practices related to clothing, shelter, and food. Human culture interacts with genes and the environment in complex ways, and studying genes and culture together can deepen our understanding of human evolution.

  17. Analysis and identification on the results of blood culture from 2007 to 2009%2007至2009年血培养检测结果分析与判断

    Institute of Scientific and Technical Information of China (English)

    沈思娣; 唐建英

    2011-01-01

    目的 通过对血培养阳性的分析,减少对污染瓶的误判,提高与临床的沟通能力,把有限的医疗资源用的更好.方法 把已抽血的专用培养瓶放入BacT/ALERT 3D select全自动血培养仪中,仪器报阳性经VITEK-32细菌培养鉴定仪鉴定到种.结果 9687瓶次血培养中有714瓶有细菌生长,分离率为7.37%;明确污染140瓶,污染率为19.61%.结论 血培养有细菌生长并不一定是患者真正感染的细菌,一定要认真分析.%Objective To reduce the misjudgment of polluted bottles by analyzing the positive samples of blood culture, and improve the communication with the clinic and utilize the limited medical resources more efficiently.Methods The bottles with blood were put into the BacT/ALERT 3D select automatic blood culture system to detect the positive samples. The VITEK-32 bacterial culture identification analyzer was used to identify each isolate. Results Among the 9 687 bottles, bacterial growth was found in 714 bottles, with the isolation rate of 7.37%. 140 bottles were identified as polluted, with the pollution rate 19.61%. Conclusions Bacteria could grow in the blood culture, but they may not cause the infection of the patients. It should make a careful analysis.

  18. Cheese production using kefir culture entrapped in milk proteins.

    Science.gov (United States)

    Dimitrellou, Dimitra; Kandylis, Panagiotis; Kourkoutas, Yiannis; Koutinas, Athanasios A; Kanellaki, Maria

    2015-05-01

    The aim of the present study was to evaluate the use of kefir culture entrapped in casein and in whey protein as starter cultures for the production of Feta-type cheese. Microbiological analysis showed that counts of enterobacteria, coliforms, and staphylococci were significantly reduced due to kefir culture. In addition, the effect of kefir culture on the formation of volatile compounds, such as esters, organic acids, alcohols, carbonyl compounds, and lactones, was also investigated using the SPME GC/MS technique. Cheese samples produced with kefir culture entrapped in milk proteins presented improved profile of aroma-related compounds. Principal component analysis of the results indicated that the volatile composition of the different cheese types was dependent on the nature of the starter culture. Finally, the sensory evaluation showed that the products produced with kefir culture had a soft, fine taste, and were of improved quality.

  19. Culture-sensitive psychotraumatology

    Directory of Open Access Journals (Sweden)

    Ulrich Schnyder

    2016-07-01

    Full Text Available Background: Although there is some evidence of the posttraumatic stress disorder (PTSD construct's cross cultural validity, trauma-related disorders may vary across cultures, and the same may be true for treatments that address such conditions. Experienced therapists tailor psychotherapy to each patient's particular situation, to the nature of the patient's psychopathology, to the stage of therapy, and so on. In addition, culture-sensitive psychotherapists try to understand how culture enhances the meaning of their patient's life history, the cultural components of their illness and help-seeking behaviors, as well as their expectations with regard to treatment. We cannot take for granted that all treatment-seeking trauma survivors speak our language or share our cultural values. Therefore, we need to increase our cultural competencies. Methods: The authors of this article are clinicians and/or researchers from across the globe, working with trauma survivors in various settings. Each author focused on one or more specific cultural aspects of working with trauma survivors and highlighted the following aspects. Results: As a result of culture-specific individual and collective meanings linked to trauma and trauma-related disorders survivors may be exposed to (self-stigma in the aftermath of trauma. Patients who are reluctant to talk about their traumatic experiences may instead be willing to write or use other ways of accessing the painful memories such as drawing. In other cultures, community and family cohesion are crucial elements of recovery. While awareness of culture-specific aspects is important, we also need to beware of premature cultural stereotyping. When disseminating empirically supported psychotherapies for PTSD across cultures, a number of additional challenges need to be taken into account: many low and middle income countries have very limited resources available and suffer from a poor health infrastructure. Conclusions: In summary

  20. Cultural psychology.

    Science.gov (United States)

    Heine, Steven J; Ruby, Matthew B

    2010-03-01

    Humans are a cultural species, constantly navigating a complex web of culturally bound practices, norms, and worldviews. This article provides a brief overview of the relatively young field of cultural psychology, which investigates the many ways psychology and culture interweave with one another. Highlighting the cultural nature of the human species, it draws upon research on cultural evolution, enculturation, and developmental processes. This review further summarizes a number of cultural differences in how people perceive the self, and the behavioral consequences that follow from these differences, in the domains of internal and external attribution styles, motivations for self-enhancement, approach/avoidance, primary and secondary control, as well as motivations for distinctiveness and conformity. Additionally, the review discusses research on the intersection of culture and emotion, as well as cultural differences in cognition, perception, and reasoning. Copyright © 2010 John Wiley & Sons, Ltd. For further resources related to this article, please visit the WIREs website. Copyright © 2010 John Wiley & Sons, Ltd.

  1. Blockbusters as vehicles for cultural debate in cultural journalism

    DEFF Research Database (Denmark)

    From, Unni; Kristensen, Nete Nørgaard

    2014-01-01

    cultural approach in cultural journalism since the mid-20th century. The analysis shows that the negotiation and definition of the blockbuster as cultural phenomenon has changed; whereas it was originally, critically, interpreted as a sign of cultural imperialism, Americanisation, or artistic decline...... at different times provides different reflective spaces for popular culture; and that contemporary cultural journalism continues to be critical and contemplative at the same time as it provides cultural service and infotainment. Consequently, the analysis also illustrates how the role of journalists...

  2. Cultural commons and cultural evolution

    OpenAIRE

    Giangiacomo Bravo

    2010-01-01

    Culture evolves following a process that is akin to biological evolution, although with some significant differences. At the same time culture has often a collective good value for human groups. This paper studies culture in an evolutionary perspective, with a focus on the implications of group definition for the coexistence of different cultures. A model of cultural evolution is presented where agents interacts in an artificial environment. The belonging to a specific memetic group is a majo...

  3. Micro fluidic System for Culturing and Monitoring of Neuronal Cells and Tissue

    DEFF Research Database (Denmark)

    Bakmand, Tanya; Waagepetersen, Helle S.

    . Tests show that the function of neurons cultured on PNWs lies closer to neurons in vivo than neurons cultured on conventional plastic substrates. The second part of the thesis describes a fluidic system for culturing of brain slices. It describes the fabrication and use of the system as well as results...... for culturing of brain tissue. The second goal was to develop a sensor system with the potential for incorporation into both conventional culture systems and fluidic culturing systems. The third and final goal of this project was to develop a system for culturing of neuronal cells with the possibility...... neuronal cells on a Peptide Nano Wires (PNW) modified substrate aiming to bring conventional neuronal cultures closer to mimic the in vivo situation. The work describes both the fabrication of the culture substrates and results comparing the performance of PNWcultured neurons and conventional cultures...

  4. Evaluation of culture techniques and bacterial cultures from uroliths.

    Science.gov (United States)

    Perry, Leigh A; Kass, Philip H; Johnson, Dee L; Ruby, Annette L; Shiraki, Ryoji; Westropp, Jodi L

    2013-03-01

    The association between urolithiasis and growth of bacteria in the urine or urolith has not been recently evaluated in the past 15 years, and the effects of antimicrobial administration on urolith cultures have not been reported. As well, laboratory techniques for urolith cultures have not been critically evaluated. The objectives of the current study were to 1) report bacterial isolates from uroliths and their association with signalment, urolith composition, antimicrobial use, and urine cultures and 2) evaluate laboratory techniques for urolith cultures. For the first objective, a retrospective search of bacterial isolates cultured from uroliths submitted to the laboratory as well as the signalment, urine culture results, and antimicrobial use were recorded. For the second objective, 50 urolith pairs were cultured by washing each urolith either 1or 4 times and culturing the core. Five hundred twenty canine and 168 feline uroliths were reviewed. Struvite-containing uroliths had an increased prevalence of a positive culture compared to nonstruvite-containing uroliths (P culture results and previous antimicrobial administration was found (P = 0.41). Eighteen percent of cases with negative urine cultures had positive urolith cultures. There was no significant difference in core culture results whether the urolith was washed 1 or 4 times (P = 0.07). Urolith culture outcome was not always influenced by previous antimicrobial administration, and bacterial culture of a urolith may not yield the same results as those obtained from the urine. The modified protocol, which requires less time and expense for urolith cultures, may be an acceptable alternative.

  5. Factors relating to the feeling of school avoidance among elementary school children: Results from the MEXT (Ministry of Education, Culture, Sports, Science and Education) Super Shokuiku School Project.

    Science.gov (United States)

    Akimoto, Masahiro; Sekine, Michikazu; Yamada, Masaaki; Tatsuse, Takashi

    2017-01-01

    up (OR 1.64, 95%CI [1.30-2.06]), being unsatisfied with their own health (OR 1.43, 95%CI [1.10-1.87]), and dislike for playing outside (OR 1.62, 95%CI [1.05-2.52]).Conclusions This study showed that feeling of school avoidance was significantly correlated with several lifestyle factors. School health activities aiming at establishing desirable lifestyles for children could reduce the number of children who have the feeling of school avoidance.

  6. Differential sensitivity of aerobic gram-positive and gram-negative microorganisms to 2,4,6-trinitrotoluene (TNT) leads to dissimilar growth and TNT transformation: Results of soil and pure culture studies

    Energy Technology Data Exchange (ETDEWEB)

    Fuller, M.E.; Manning, J.F. Jr.

    1996-07-30

    The effects of 2,4,6-trinitrotoluene (TNT) on indigenous soil populations and pure bacterial cultures were examined. The number of colony-forming units (CFU) appearing when TNT-contaminated soil was spread on 0.3% molasses plates decreased by 50% when the agar was amended with 67 {mu}g TNT mL{sup -1}, whereas a 99% reduction was observed when uncontaminated soil was plated. Furthermore, TNT-contaminated soil harbored a greater number of organisms able to grow on plates amended with greater than 10 {mu}g TNT mL{sup -1}. The percentage of gram-positive isolates was markedly less in TNT-contaminated soil (7%; 2 of 30) than in uncontaminated soil (61%; 20 of 33). Pseudomonas aeruginosa, Pseudomonas corrugate, Pseudomonasfluorescens and Alcaligenes xylosoxidans made up the majority of the gram-negative isolates from TNT-contaminated soil. Gram-positive isolates from both soils demonstrated marked growth inhibition when greater than 8-16 {mu}g TNT mL{sup -1} was present in the culture media. Most pure cultures of known aerobic gram-negative organisms readily degraded TNT and evidenced net consumption of reduced metabolites. However, pure cultures of aerobic gram-positive bacteria were sensitive to relatively low concentrations of TNT as indicated by the 50% reduction in growth and TNT transformation which was observed at approximately 10 {mu}g TNT mL{sup -1}. Most non-sporeforming gram-positive organisms incubated in molasses media amended with 80 {mu}g TNT mL{sup -1} or greater became unculturable, whereas all strains tested remained culturable when incubated in mineral media amended with 98 {mu}g TNT mL{sup -1}, indicating that TNT sensitivity is likely linked to cell growth. These results indicate that gram-negative organisms are most likely responsible for any TNT transformation in contaminated soil, due to their relative insensitivity to high TNT concentrations and their ability to transform TNT.

  7. Organizational Culture and the Military

    Science.gov (United States)

    2007-11-02

    This project reviews key concepts of organizational culture and examines how our military culture may change as a result of various social and...of the society it is sworn to defend. This study examined the military’s organizational culture through the use of an organizational culture /climate

  8. Repellent Culture.

    Science.gov (United States)

    Carroll, Jeffrey

    2001-01-01

    Considers defining "culture," noting how it is difficult to define because those individuals defining it cannot separate themselves from it. Relates these issues to student writing and their writing improvement. Addresses violence in relation to culture. (SG)

  9. Culturing Protozoa.

    Science.gov (United States)

    Stevenson, Paul

    1980-01-01

    Compares various nutrient media, growth conditions, and stock solutions used in culturing protozoa. A hay infusion in Chalkey's solution maintained at a stable temperature is recommended for producing the most dense and diverse cultures. (WB)

  10. Culturing Protozoa.

    Science.gov (United States)

    Stevenson, Paul

    1980-01-01

    Compares various nutrient media, growth conditions, and stock solutions used in culturing protozoa. A hay infusion in Chalkey's solution maintained at a stable temperature is recommended for producing the most dense and diverse cultures. (WB)

  11. Measuring Cultural Diversity

    DEFF Research Database (Denmark)

    Patsiurko, Natalka; Campbell, John L.; Hall, John A.

    2012-01-01

    Many claim that national economic success depends upon cultural homogeneity. We collect new time-series data and develop new measures of ethnic, linguistic and religious fractionalization for the OECD countries. We show that cultural diversity may vary by type across countries and over short...

  12. Etruscan Culture Exhibition

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    EARLY this year an exhibition on the ancient civilization of Etruria was held at the Beijing-based China Millennium Monument.The theme of the exhibition was Etruscan Culture and on show were the most representative cultural and historical relics of this ancient civilization unearthed in the past 20 years. The 349 exhibits from various

  13. Safety culture

    Energy Technology Data Exchange (ETDEWEB)

    Keen, L.J. [Canadian Nuclear Safety Commission, Ottawa, Ontario (Canada)

    2003-07-01

    Safety culture has become a topic of increasing interest for industry and regulators as issues are raised on safety problems around the world. The keys to safety culture are organizational effectiveness, effective communications, organizational learning, and a culture that encourages the identification and resolution of safety issues. The necessity of a strong safety culture places an onus on all of us to continually question whether the safety measures already in place are sufficient, and are being applied. (author)

  14. Group cohesion: Relationships with work team culture.

    Science.gov (United States)

    Sánchez, José C; Yurrebaso, Amaia

    2009-02-01

    The aim of this study was to examine the relationships between group cohesion and the perceived culture and desired culture of work teams. Two separate studies were carried out with a time interval of one year. The first study had a sample of 50 work teams belonging to different organizations and the second study had a sample of 75 work teams. We used Lindell's index of agreement to estimate the culture and cohesion variables. Multiple and hierarchical regression analysis in both studies confirmed our initial hypothesis, showing that perceived and desired culture were positively related to group cohesion, and that culture gap had a negative association with cohesion. To a lesser extent, control variables such as job tenure and gender had a positive relationship with cohesion. The implications of these results are discussed along with the importance of considering the group level as analysis.

  15. Cultural clashes

    National Research Council Canada - National Science Library

    2001-01-01

    ... is essentially a cultural, not religious, practice. Siddiq Bazarwala Singapore From Georgina Dubourcq Sir: While I don't disagree with Dr Dalrymple's comments about the way Muslim culture can treat women, I wonder how a culture which condones lap-dancing, pornographic videos and often turns a blind eye to under-age prostitution can really feel superior. I...

  16. Handling Culture

    NARCIS (Netherlands)

    Pieter van Nispen tot Pannerden

    2011-01-01

    The article indicates how companies may prepare for and deal with cultural differences. Because the research base is still rather limited an overall perspective may not be realised. After discussing definitions and concepts of culture, as well as values, cultural differences between states are discu

  17. Handling Culture

    NARCIS (Netherlands)

    Nispen tot Pannerden, P.J.M. van

    2011-01-01

    The article indicates how companies may prepare for and deal with cultural differences. Because the research base is still rather limited an overall perspective may not be realised. After discussing definitions and concepts of culture, as well as values, cultural differences between states are discu

  18. Beyond Culture.

    Science.gov (United States)

    Barron, Daniel D.

    1993-01-01

    Discusses the lack of literature relating to cultural differences and school library media programs and reviews the book "Beyond Culture" by Edward T. Hall. Highlights include the population/environment crisis, cultural literacy, the use of technology, and Marshall McLuhan's idea of the global village. (LRW)

  19. Recycling as a Result of "Cultural Greening"?

    Science.gov (United States)

    Flagg, Julia A.; Bates, Diane C.

    2016-01-01

    Purpose: This study aims to test whether faculty and students who have developed the most pro-environmental values and concerns are also the most likely to reduce the on-campus waste stream. It does so by using the theory of ecological modernization. Design/methodology/approach: Questionnaires were created and disseminated to a representative…

  20. Cultural bases for self-evaluation: seeing oneself positively in different cultural contexts.

    Science.gov (United States)

    Becker, Maja; Vignoles, Vivian L; Owe, Ellinor; Easterbrook, Matthew J; Brown, Rupert; Smith, Peter B; Bond, Michael Harris; Regalia, Camillo; Manzi, Claudia; Brambilla, Maria; Aldhafri, Said; González, Roberto; Carrasco, Diego; Paz Cadena, Maria; Lay, Siugmin; Schweiger Gallo, Inge; Torres, Ana; Camino, Leoncio; Özgen, Emre; Güner, Ülkü E; Yamakoğlu, Nil; Silveira Lemos, Flávia Cristina; Trujillo, Elvia Vargas; Balanta, Paola; Macapagal, Ma Elizabeth J; Cristina Ferreira, M; Herman, Ginette; de Sauvage, Isabelle; Bourguignon, David; Wang, Qian; Fülöp, Márta; Harb, Charles; Chybicka, Aneta; Mekonnen, Kassahun Habtamu; Martin, Mariana; Nizharadze, George; Gavreliuc, Alin; Buitendach, Johanna; Valk, Aune; Koller, Silvia H

    2014-05-01

    Several theories propose that self-esteem, or positive self-regard, results from fulfilling the value priorities of one's surrounding culture. Yet, surprisingly little evidence exists for this assertion, and theories differ about whether individuals must personally endorse the value priorities involved. We compared the influence of four bases for self-evaluation (controlling one's life, doing one's duty, benefitting others, achieving social status) among 4,852 adolescents across 20 cultural samples, using an implicit, within-person measurement technique to avoid cultural response biases. Cross-sectional and longitudinal analyses showed that participants generally derived feelings of self-esteem from all four bases, but especially from those that were most consistent with the value priorities of others in their cultural context. Multilevel analyses confirmed that the bases of positive self-regard are sustained collectively: They are predictably moderated by culturally normative values but show little systematic variation with personally endorsed values.

  1. Advertising cultures

    DEFF Research Database (Denmark)

    Malefyt, Timothy de Waal; Moeran, Brian

    The growth, success and secrets of advertizing are legendary. Advertizing agencies ceaselessly churn out evermore sophisticated campaigns that, when successful, manage to capture the every essence of consumer desire. The secrets of advertizing are perhaps best understood by turning......, but exposes, through in-depth accounts based on personal experience, the inner workings of the advertizing industry. How do adverts manage to capture "real" life? What issues do agencies have to consider when using an advert in a range of different countries? What specific methods are used to persuade us...... to the relationship between advertizing and anthropology. The link between them may come as a surprise to those who consider advertizing to be firmly rooted in commerce and anthropology in culture. Through the lens of anthropologists, this book not only shows how anthropology and advertizing are connected...

  2. Comparison of the results between quantitative procalcitonin detection and blood culture%血清降钙素原定量检测与血培养结果的比较

    Institute of Scientific and Technical Information of China (English)

    王凯飞; 沈定霞; 刘朝军; 叶丽艳; 王磊利; 张有江

    2012-01-01

    目的 探讨降钙素原(PCT)定量检测在血流感染患者中的临床应用价值.方法 采用回顾性研究,对解放军总医院1066例患者同时送检血液培养和PCT检测的结果进行分析,其中有效结果1010例.比较血培养结果与血清PCT浓度的关系及PCT浓度在革兰阴性细菌、革兰阳性细菌及假丝酵母菌之间的差异.并且对33例进行过多次PCT检测的血培养阳性患者预后进行了分析.三组间PCT值差异比较采用Mann-Whitney U检验;死亡率比较采用Fisher's精确概率法.结果 血培养阴性患者PCT为0.37(0.11 ~1.67) μg/L.血培养阳性患者PCT水平为2.24(0.57~11.59)μg/L;PCT在革兰阴性细菌、革兰阳性细菌及假丝酵母菌中的阳性率分别为86.6%、72.0%及75.7%.在33例多次PCT检测的血培养阳性患者中,PCT进行性减低者预后较好,PCT均值>5 μg/L者预后较差.结论 定量检测PCT可作为快速排除和诊断血流感染的辅助检测手段.观察PCT变化趋势对预后判断有一定的参考价值.%Objective To evaluate the application value of the quantitative procalcitonin (PCT) test in bloodstream infection.Methods Of 1066 patients with blood culture and PCT detection were collected in our hospital,retrospectively,1010 were effective cases.The relationship between blood culture results and serum PCT levels was investigated.PCT levels in gram-negative bacterial infection,gram-positive bacterial infection and candidiasis were compared.The prognosis of 33 blood culture positive patients with repeated PCT detection results were analyzed.Mann-Whitney U test was used to compare the PCT value among the three groups,and Fisher' s test was used to compare the death rate among the three groups.Results In the patients with negative blood culture results,the median of PCT was 0.37 (0.11 - 1.67) μg/L.But in the patients with positive blood culture results,the median of PCT were 2.24(0.57 -11.59) μg/L The positive rate of PCT in gram

  3. Culture, attention, and emotion.

    Science.gov (United States)

    Grossmann, Igor; Ellsworth, Phoebe C; Hong, Ying-yi

    2012-02-01

    This research provides experimental evidence for cultural influence on one of the most basic elements of emotional processing: attention to positive versus negative stimuli. To this end, we focused on Russian culture, which is characterized by brooding and melancholy. In Study 1, Russians spent significantly more time looking at negative than positive pictures, whereas Americans did not show this tendency. In Study 2, Russian Latvians were randomly primed with symbols of each culture, after which we measured the speed of recognition for positive versus negative trait words. Biculturals were significantly faster in recognizing negative words (as compared with baseline) when primed with Russian versus Latvian cultural symbols. Greater identification with Russian culture facilitated this effect. We provide a theoretical discussion of mental processes underlying cultural differences in emotion research.

  4. Leveraging organisational cultural capital

    Directory of Open Access Journals (Sweden)

    R Scheel

    2007-01-01

    Full Text Available Organisational culture discourse mandates a linear approach of diagnosis, measurement and gap analysis as standard practice in relation to most culture change initiatives. Therefore, a problem solving framework geared toward “fixing�? and/or realigning an organisation’s culture is usually prescribed. The traditional problem solving model seeks to identify gaps between current and desired organisational cultural states, inhibiting the discovery of an organisation’s unique values and strengths, namely its cultural capital. In pursuit of discovering and leveraging organisational cultural capital, a descriptive case study is used to show how an Appreciative Inquiry process can rejuvenate the spirit of an organisation as a system-wide inquiry mobilises a workforce toward a shared vision.

  5. Cryopreservation of olive embryogenic cultures.

    Science.gov (United States)

    Sánchez-Romero, C; Swennen, R; Panis, B

    2009-01-01

    The aim of this work was to optimize a protocol for the cryopreservation of embryogenic cultures of olive (Olea europaea L.). Exposure time to loading solution and PVS2 significantly influenced the regrowth rate of both organized and non-organized tissues. Organized tissues were more sensitive to prolonged treatments with vitrification solutions compared to non-organized tissues. Three cryopreservation protocols were compared using non-organized tissues: the "classical" vitrification protocol, an ultra-fast freezing method using droplet vitrification on aluminium foil strips and a "classical" slow freezing method (1 degree C per min). The best results were obtained using the droplet vitrification method after a 60 min dehydration period with PVS2. Under these conditions, all cryopreserved cultures showed renewed embryogenesis six weeks after thawing. A long-term (7-8 weeks) sucrose preculture had a significant effect on the initial response of the cultures, allowing particularly to protect cells against the toxic effects of the vitrification solution.

  6. The Test of Strategic Culture

    DEFF Research Database (Denmark)

    Dalgaard-Nielsen, Anja

    2005-01-01

    Germany was the first country to issue a categorical refusal to support the US-led war in Iraq. Some have interpreted this as the result of a clash between the strategic cultures of Germany and the USA, others as a sign that a more nationalistic and assertive Germany is emerging. This article...... explains the apparently contradictory aspects of Germany’s stance on Iraq by identifying two competing strands within Germany’s strategic culture. It concludes that the German refusal signals neither a reversion to a pacifist stance nor that Germany is in a process of shedding the bonds and alliances...... that have so far framed the reunified Germany’s military policy. Iraq simply showed that Germany, like most other countries, has conditions that have to be met – in Germany’s case, conditions flowing from the coexistence of two competing schools of thought within Germany’s strategic culture....

  7. Culturing Fundamentals Used To Design And Execute A Long-Term Multi-stressor Experiment To Assess Impact Of Deoxygenation, Ocean Acidification, And Warming On Benthic Foraminiferal Community Composition, Growth, And Carbonate Yield: Design And Results

    Science.gov (United States)

    Bernhard, J. M.; Wit, J. C.

    2015-12-01

    The geochemistry recorded in carbonate foraminiferal tests (shells) is often used as proxy for past oceanographic events and environments. By understanding past oceanic and climatic conditions, we can better predict future climate scenarios, a relevant ability in these times of global change. The fact that foraminifera are biological entities can be pivotal for understanding their geochemical records. Thus, growing foraminifera under known physicochemical conditions and analyzing the geochemistry of their cultured carbonate can yield insightful perspectives for proxy refinement and development. Because parameters often co-vary in nature, proper proxy calibration can only be done with materials grown in strictly controlled and known environments. This presentation will review the various crucial aspects of foraminiferal maintenance and culturing, especially from the perspective of proxy development. These fundamentals were used to design a long-term multi-stressor experiment with oxygen, pCO2 (pH), and temperature as variables to test the single, double or triple threats of deoxygenation, ocean acidification, and oceanic warming. Results on assemblage composition, survivorship and growth of a continental shelf benthic foraminiferal community will be presented. Although one agglutinated morphospecies grew in each of the five treatments, growth of individual calcareous species was more restricted. Initial results indicate that pCO2 was not the factor that impacted communities most. Supported in part by NSF OCE-1219948.

  8. Cultural Aspects of Secrecy in Global Economy

    Science.gov (United States)

    Serradell-Lopez, Enric; Cavaller, Victor

    The main objective of this paper is to provide greater understanding of the nature of secrecy in firms. It presents an effort to develop some links between management of the secrecy and its relationship with culture. Using measures from Hofstede's work, we have linked some dimensions of national culture with CIS 3 UE survey database. The results show that some attributes of the culture as Masculinity and Uncertainty Avoidance have impact on the tendency of the firms for not to patent and maintain secrecy of their innovations.

  9. Culture moderates children's responses to ostracism situations.

    Science.gov (United States)

    Over, Harriet; Uskul, Ayse K

    2016-05-01

    Across a series of studies, we investigated cultural differences in children's responses to ostracism situations. Working with the children of farmers and herders, we focused on how painful children estimate ostracism to be. Study 1a showed that 4- to 8-year-old children from a socially interdependent farming community estimated ostracism to be less painful than did children from an independent herding community. Study 1b showed that this cultural difference was specific to social pain and did not apply to physical pain. Study 2 replicated the results of Study 1a and showed that individual differences in parents' level of social interdependence mediated the relationship between cultural group and how painful children estimate ostracism to be. Study 3 replicated this effect again and showed that children's tendency to recommend seeking social support following ostracism mediated the relationship between cultural group and the perceived pain of being excluded. Finally, Study 4 investigated cultural differences in moral responses to ostracism and showed that children from the farming community punished an individual who ostracized someone else less harshly than did children from the independent herding community. Thus different economic cultures are associated with striking differences in social interdependence and responses to ostracism from early in development. (PsycINFO Database Record

  10. Características del liderazgo en el sector de relaciones públicas. Resultados comparativos del Cross-Cultural Study of Leadership in Public Relations and Communication Management /Characteristics of Leadership in Public Relations. A comparison of results

    Directory of Open Access Journals (Sweden)

    Cristina Navarro Ruiz

    2013-05-01

    Full Text Available ResumenEste artículo ofrece un análisis comparativo de los factores de liderazgo en el sector de las relaciones públicas y la gestión de la comunicación entre España y el conjunto de los 23 países que han participado en el Cross-Cultural Cultural Study of Leadership in Public Relations and Communication Management, promovido por la Universidad de Alabama y financiado por IBM y Heyman Associates. Siguiendo el modelo conceptual de Meng y Berger (2011, se abordan los desafíos que afrontan las relaciones públicas en la actualidad, así como los requisitos y características de los ‘líderes excelentes’. Los resultados obtenidos suponen un avance en el conocimiento científico sobre el liderazgo en la gestión de comunicación, un tema aún de escaso desarrollo en la literatura académica internacional y especialmente poco explorado en España.AbstractThis paper offers a comparative analysis of leadership dimensions in public relations and communication management between Spain and a group of 23 countries which have participated in the Cross-Cultural Study of Leadership in Public relations and CommunicationManagement, conducted by the University of Alabama and financed by IBM and Heymann Associates. Following the conceptual model from Meng and Berger (2011, this paper investigates the challenges faced by public relations, as well as the prerequisites of excellent leaders. Achieved results make a breakthrough in scientific knowledge about leadership in communication management, a little developed subject in academic literature, especially in Spain.

  11. Cultural Novelty and Adjustment: Western Business Expatriates in China

    DEFF Research Database (Denmark)

    Selmer, Jan

    2006-01-01

    Western business expatriates in China. Three sociocultural adjustment variables were examined; general, interaction and work adjustment. Although a negative relationship was hypothesized between cultural novelty and the three adjustment variables, results of the hierarchical multiple regression analysis...... showed that there was no significant association between them. Although highly tentative, the suggestion that it is as difficult for business expatriates to adjust to a very similar culture as to a very dissimilar culture, is fundamental. Implications of this potentially crucial finding are discussed...

  12. THE PRELIMINARY STUDY ON LANDSCAPE CULTURE ORIENTATION AND EXPLOITATION OF THE SOUTH DONGTING LAKE WETLAND

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Dongting Lake is internationally an important wetland. We studied and summarized the conception, function, classification and current situation of the wetland-landscape culture in this region. The results showed that the culture of Dongting Lake wetland was rich in diversity, which are the Rice Cultivation Culture, high-balustrade dwelling,Nuo Culture, Ship Culture, Dragon Boat Culture, Chu Culture, Ancient Architecture Landscape, Wetland Foodstuff andCuisine Culture, Civil Art, Historic Heritage and Cultural Relics, Revolutionary Sites and Ruins, and Production andLiving Culture, etc. We also evaluated the eeo-tourism value of wetland landscape culture, and analyzed its features andorientation. The results revealed that the south Dongting Lake wetland plays a key role on the Changjiang(Yangtze) Riverreaches civilization and Chinese civilization, even has great influence on the global civilization. We summarized that thesoul of the south Dongting Lake Culture was Wetland Culture, Water Culture, Rice Cultivation and Chu Culture. Thethoughts, principles and approaches of sustainable exploitation and utilization of the wetland landscape culture were formulated and suggested.

  13. THE PRELIMINARY STUDY ON LANDSCAPE CULTURE ORIENTATION AND EXPLOITATION OF THE SOUTH DONGTING LAKE WETLAND

    Institute of Scientific and Technical Information of China (English)

    HEPing; WANGBao-zhong

    2002-01-01

    Dongting Lake is internationally an important wetland.We summarized the conception,func-tion,classification and current situation of the wetland-landscape culture in this region.The results showed that the cul-ture of Dongting Lake wetland was rich in diversity,which are the Rice Cultivation Culture,high-balustrade dwelling,Nuo Culture,Ship Culture ,Dragon Boat Culture,Chu Culture,Ancient Architecture Landscape,Wetland Foodstuff and Cuisine Culture,Civil Art.Historic Heritage and Cultural Relics,Revolutionary Sites and Ruins,and Production and Living Culture,etc.We also evaluated the eco-tourism value of wetland landscape culture,and analyzed its features and orientation.The results revealed that the south Dongting Lake wetland plays role on the Changjiang(Yangtze)River reaches civilization and Chinese civilization,even has great influence on the global civilization.We summarized that the soul of the south Dongting Lake Culture was Wetland Culture ,Water Culture,Rice Cultivation and Chu Culture.The thoughts ,principles and approaches of sustainable exploitation and utilization of the wetland landscape culture were formulat-ed and suggested.

  14. Paradigm dichotomies of international cultural relations

    OpenAIRE

    Rogač-Mijatović, Ljiljana

    2012-01-01

    International cultural relations are being discussed in terms of both theory and practice, as well as designing terms of reference 'cultural diplomacy' and 'public diplomacy'. The presented analysis shows the development of international cultural relations in a historical perspective, from cultural internationalism and cultural propaganda, to the paradigms of the Cold War post-Cold War cultural relations. The text as a key idea tackles the issue of diverse conceptions of culture in internatio...

  15. Culture-sensitive psychotraumatology

    Science.gov (United States)

    Schnyder, Ulrich; Bryant, Richard A.; Ehlers, Anke; Foa, Edna B.; Hasan, Aram; Mwiti, Gladys; Kristensen, Christian H.; Neuner, Frank; Oe, Misari; Yule, William

    2016-01-01

    Background Although there is some evidence of the posttraumatic stress disorder (PTSD) construct's cross cultural validity, trauma-related disorders may vary across cultures, and the same may be true for treatments that address such conditions. Experienced therapists tailor psychotherapy to each patient's particular situation, to the nature of the patient's psychopathology, to the stage of therapy, and so on. In addition, culture-sensitive psychotherapists try to understand how culture enhances the meaning of their patient's life history, the cultural components of their illness and help-seeking behaviors, as well as their expectations with regard to treatment. We cannot take for granted that all treatment-seeking trauma survivors speak our language or share our cultural values. Therefore, we need to increase our cultural competencies. Methods The authors of this article are clinicians and/or researchers from across the globe, working with trauma survivors in various settings. Each author focused on one or more specific cultural aspects of working with trauma survivors and highlighted the following aspects. Results As a result of culture-specific individual and collective meanings linked to trauma and trauma-related disorders survivors may be exposed to (self-)stigma in the aftermath of trauma. Patients who are reluctant to talk about their traumatic experiences may instead be willing to write or use other ways of accessing the painful memories such as drawing. In other cultures, community and family cohesion are crucial elements of recovery. While awareness of culture-specific aspects is important, we also need to beware of premature cultural stereotyping. When disseminating empirically supported psychotherapies for PTSD across cultures, a number of additional challenges need to be taken into account: many low and middle income countries have very limited resources available and suffer from a poor health infrastructure. Conclusions In summary, culture

  16. Islamic Cultural Exchanges

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The China-Indonesia Islamic Culture Expo and Art Show 2010 was held in Indonesia on July 23-25.With the theme of Peace,Friendship, Cooperation and Progress,the event also commemorated the 60th anniversary

  17. Online Italian fandoms of American TV shows

    Directory of Open Access Journals (Sweden)

    Eleonora Benecchi

    2015-06-01

    Full Text Available The Internet has changed media fandom in two main ways: it helps fans connect with each other despite physical distance, leading to the formation of international fan communities; and it helps fans connect with the creators of the TV show, deepening the relationship between TV producers and international fandoms. To assess whether Italian fan communities active online are indeed part of transnational online communities and whether the Internet has actually altered their relationship with the creators of the original text they are devoted to, qualitative analysis and narrative interviews of 26 Italian fans of American TV shows were conducted to explore the fan-producer relationship. Results indicated that the online Italian fans surveyed preferred to stay local, rather than using geography-leveling online tools. Further, the sampled Italian fans' relationships with the show runners were mediated or even absent.

  18. Culture evolves.

    Science.gov (United States)

    Whiten, Andrew; Hinde, Robert A; Laland, Kevin N; Stringer, Christopher B

    2011-04-12

    Culture pervades human lives and has allowed our species to create niches all around the world and its oceans, in ways quite unlike any other primate. Indeed, our cultural nature appears so distinctive that it is often thought to separate humanity from the rest of nature and the Darwinian forces that shape it. A contrary view arises through the recent discoveries of a diverse range of disciplines, here brought together to illustrate the scope of a burgeoning field of cultural evolution and to facilitate cross-disciplinary fertilization. Each approach emphasizes important linkages between culture and evolutionary biology rather than quarantining one from the other. Recent studies reveal that processes important in cultural transmission are more widespread and significant across the animal kingdom than earlier recognized, with important implications for evolutionary theory. Recent archaeological discoveries have pushed back the origins of human culture to much more ancient times than traditionally thought. These developments suggest previously unidentified continuities between animal and human culture. A third new array of discoveries concerns the later diversification of human cultures, where the operations of Darwinian-like processes are identified, in part, through scientific methods borrowed from biology. Finally, surprising discoveries have been made about the imprint of cultural evolution in the predispositions of human minds for cultural transmission.

  19. Primary Culture of Porcine Pancreatic Acinar Cells

    Directory of Open Access Journals (Sweden)

    Zhao X

    2001-03-01

    Full Text Available OBJECTIVE: To develop a method for the primary culture of porcine pancreatic acinar cells. INTERVENTIONS: Dispersed pancreatic acinar cells available utilizing RPMI-1640 medium containing collagenase III. After purification, the isolated acinar cells were cultured in RPMI-1640 medium with the addition of 2.5% fetal bovine serum. MAIN OUTCOME MEASURES: The morphological characteristics of acinar cells were described. (3H-thymidine incorporation of acinar cells and the activity of amylase or lipase were determined during the culture process. RESULTS: There were no remarkable morphological changes in the pancreatic acinar cells during the 20 days culture. The acini showed a tendency to gather but did not attach to the walls of the culture disks. A good (3H-thymidine incorporation of acinar cells in the primary culture was maintained. The secretion of amylase or lipase from the acini decreased with the length of time of the culture. DISCUSSION: The primary culture of acinar cells from a porcine pancreas which was carried out in this study maintained the normal morphology of the acinar cells and their ability to grow but not their secretion of amylase or lipase. The method would benefit by the further experiments on acini of porcine pancreas.

  20. Parenting, Mental Health and Culture: A Fifth Cross-Cultural Research on Parenting and Psychological Adjustment of Children

    Science.gov (United States)

    Dwairy, Marwan; Achoui, Mustafa; Filus, Anna; Rezvan nia, Parissa; Casullo, Maria Martina; Vohra, Neharika

    2010-01-01

    We examined psychological disorders across cultures and their associations with parental factors (control, inconsistency, and rejection). A questionnaire assessing psychological disorders was administered to male and female adolescents in nine countries. The results showed that psychological disorders differ across cultures. Parental factors are…

  1. Updating cultural capital theory

    DEFF Research Database (Denmark)

    Prieur, Annick; Savage, Mike

    2011-01-01

    This paper considers how the analysis of cultural engagement can be elaborated through a reworking of the concept of cultural capital, as originally derived from Bourdieu’s (1984) Distinction. Drawing on detailed studies of the UK and Aalborg, Denmark, we show that despite the weakening of ‘‘high......This paper considers how the analysis of cultural engagement can be elaborated through a reworking of the concept of cultural capital, as originally derived from Bourdieu’s (1984) Distinction. Drawing on detailed studies of the UK and Aalborg, Denmark, we show that despite the weakening......, and art); and (c) an international and a local or national orientation stand out as the most important. We discuss whether these oppositions can usefully be conceptualised as cultural capital. We argue that such a conceptualisation demands, first, that cultural capital is understood in relative rather...... than absolute terms, and that a field analytic perspective provides the means of understanding cultural capital as such a relative entity. Second, the move from the empirical observation of oppositions to the conceptualisation of cultural capital also demands that the functioning of features...

  2. Spatial Culture

    DEFF Research Database (Denmark)

    2012-01-01

    – 2006. The essays published here allow us to subdivide the field of spatial culture into five major domains, summarized in the titles of chapters in the book: ”Perception and Strategies: Architecture”, ”Politics and Poetics: Urban Spaces”, ”Movements and Cityscape: Textuality”, ”Crisis and Construction......Spatial Culture – A Humanities Perspective Abstract of introductory essay by Henrik Reeh Secured by alliances between socio-political development and cultural practices, a new field of humanistic studies in spatial culture has developed since the 1990s. To focus on links between urban culture...... and modern society is, however, an intellectual practice which has a much longer history. Already in the 1980s, the debate on the modern and the postmodern cited Paris and Los Angeles as spatio-cultural illustrations of these major philosophical concepts. Earlier, in the history of critical studies, the work...

  3. When you have lived in a different culture, does returning 'home' not feel like home? Predictors of psychological readjustment to the heritage culture.

    Science.gov (United States)

    Altweck, Laura; Marshall, Tara C

    2015-01-01

    Many repatriates find it challenging to readjust to their heritage culture after spending a significant period of time abroad. Research on predictors of readjustment, however, remains limited. The present study in particular investigated the identification of third culture individuals (TCIs) - that is, individuals who spent their formative years outside of their heritage culture - with an abstract, third culture. Our findings demonstrated that TCIs' identification with the third culture was empirically distinct from that of the heritage and host cultures. The present study further examined whether several variables - sojourner type (TCI vs. non-TCI), perceived conflict between heritage and host culture, perceived cultural distance, and cultural identification with heritage and other cultures - predicted psychological readjustment (stress, anxiety, depression and overall psychological readjustment). The results showed that strong heritage culture identification was associated with better psychological readjustment, whereas cultural conflict was generally associated with poorer readjustment. Furthermore, sojourner type significantly moderated the latter association, such that cultural conflict predicted the stress aspect of psychological readjustment for non-TCIs, but not for TCIs. As the present investigation is the first study to empirically establish identification with a 'third culture' we discuss implications for the literature on third culture individuals and psychological adjustment upon re-entry.

  4. When you have lived in a different culture, does returning 'home' not feel like home? Predictors of psychological readjustment to the heritage culture.

    Directory of Open Access Journals (Sweden)

    Laura Altweck

    Full Text Available Many repatriates find it challenging to readjust to their heritage culture after spending a significant period of time abroad. Research on predictors of readjustment, however, remains limited. The present study in particular investigated the identification of third culture individuals (TCIs - that is, individuals who spent their formative years outside of their heritage culture - with an abstract, third culture. Our findings demonstrated that TCIs' identification with the third culture was empirically distinct from that of the heritage and host cultures. The present study further examined whether several variables - sojourner type (TCI vs. non-TCI, perceived conflict between heritage and host culture, perceived cultural distance, and cultural identification with heritage and other cultures - predicted psychological readjustment (stress, anxiety, depression and overall psychological readjustment. The results showed that strong heritage culture identification was associated with better psychological readjustment, whereas cultural conflict was generally associated with poorer readjustment. Furthermore, sojourner type significantly moderated the latter association, such that cultural conflict predicted the stress aspect of psychological readjustment for non-TCIs, but not for TCIs. As the present investigation is the first study to empirically establish identification with a 'third culture' we discuss implications for the literature on third culture individuals and psychological adjustment upon re-entry.

  5. Culture Shock

    Institute of Scientific and Technical Information of China (English)

    宋文玲

    2004-01-01

    Specialists say that it is not easy to get used to life in a new culture.“Culture shock”is the term these specialists use when talking about the feelings that people have in a new environment.There are three stages of culture shock,say the specialists.In the first stage,the newcomers like their new environment,Then when the fresh experience

  6. Cultural diversity

    OpenAIRE

    Raghavan, Raghu

    2011-01-01

    The concept of cultural diversity has emerged as an influential one having impact on multiple policy and legal instruments especially following the adoption of the UNESCO Convention on the Protection and Promotion of the Diversity of Cultural Expressions in 2005. The discussions on its appropriate implementation are however profoundly fragmented and often laden with political considerations. The present brief paper offers some thoughts on the meaning of cultural diversity and its implementati...

  7. Primary culture of human skin melanocyte and comparison of culture in the presence and absence of phorbol ester

    Directory of Open Access Journals (Sweden)

    Reza Yarani

    2013-06-01

    Full Text Available Background: Primary culture takes place following the cell isolation from tissues. Isolation and culture of melanocytes based on their roll in the protection of body against hazardous sun rays, production of skin, cornea and hair color is really important. This study was done to set isolation, culture and proliferation of melanocytes from children foreskin and adult eyelashes, and also comparison of two types of melanocyte culture medium. Methods: Human foreskin and eyelash samples were used for melanocyte isolation and culture. After isolation of epidermis from dermis, epidermis cell suspensions were prepared by enzymatic digestion. The isolated cells were cultured in two melanocyte selective culture media. Immunocytochemistary and reverse transcription-polymerase chain reaction (RT-PCR assays were used for confirmation of isolated and cultured melanocytes. Results: Our results indicated that isolated melanocyte cultured in the selective medium without phorbol esters is better than the melanocytes cultured in selective medium cont-aining phorbol esters not only morphologically but also physiologically and from the aspect of cell adhesion. In addition, the results showed that isolated melanocyte from adult eyelashes are more dendritic than melanocytes isolated from children foreskin. Conversely, our results indicated that the number of cell passages in melanocyte isolat-ed from foreskin is more than melanocytes isolated from adult eyelashes. Conclusion: Melanocytes cultured in selective medium containing convenient growth factors in absence of phorbol esters show more native physiological and adhesive properties. In addition, melanocyte isolated from younger tissues such as foreskin have better proliferative and sub-culturing properties so we suggest isolation and culture of younger tissues.

  8. Coincidence of sputum smear and culture results of children with lower respiratory tract infection%儿童下呼吸道感染痰涂片与痰培养结果一致性研究

    Institute of Scientific and Technical Information of China (English)

    张艳; 王红梅; 蒋元琴; 陈虹宇; 赵瑞珍; 马东礼

    2015-01-01

    Objective To compare sputum smear and culture results in children with lower respiratory infection, realize the laboratory diagnostic value of sputum smear,and analyze the distribution and antimicrobial resistance of cultured pathogens in infection,so as to guide rational antimicrobial use in clinic.Methods Qualified sputum speci-mens collected from hospitalized children with lower respiratory tract infection from March to May 2014 were con-ducted gram-staining after smeared,meanwhile,bacterial culture,identification and antimicrobial susceptibility tes-ting of sputum specimens were performed,gram-staining microscopic examination and culture results were com-pared.Results The overall coincidence rate of sputum smear and culture results was 67.43%(n =617),positive rate of sputum culture was 65.90%(n =603);730 pathogenic strains were isolated,the top 3 isolated pathogens were Haemophilus parainfluenzae (36.17%),Streptococcus pneumoniae (24.38%),and Moraxella catarrhalis (13.29%).β-lactamase-producing rates of Haemophilus parainfluenzae ,Haemophilus influenzae ,and Moraxella catarrhalis were 44.70%(118/264),45.83%(11/24),and 93.81 %(91/97)respectively.Streptococcus pneumoniae were susceptible to most antimicrobial agents,while resistant rates to erythromycin,tetracycline,and compound sulfamethoxazole were high, vancomycin-, linezolid-, and/or moxifloxacin-resistant strains were not found. Conclusion Sputum smear has certain laboratory diagnostic value,gram-staining is recommended while sputum cul-ture is performed,antimicrobial agents should be used rationally according to antimicrobial susceptibility testing re-sults,so as to reduce the emergence of antimicrobial-resistant organisms.%目的:比较儿童下呼吸道感染痰涂片与痰培养结果,了解痰涂片的实验室诊断价值,同时对痰培养感染病原菌的分布及耐药性进行分析,以指导临床合理用药。方法收集2014年3—5月某院915份下呼吸道感染住院患儿的合

  9. Isolation and culture of chicken primordial follicles.

    Science.gov (United States)

    Leghari, Imdad Hussain; Zhao, Dan; Mi, Yuling; Zhang, Caiqiao

    2015-10-01

    The establishment of a primordial follicle culture system is important for the study of follicular development. Hence, the objective of this study was to isolate chicken primordial follicles and establish culture methods. Ovaries from 2-wk-old chickens were treated with trypsin-EDTA, collagenase II, or collagenase type IA, along with a mechanical isolation technique. Isolated follicles were cultured under different conditions. Results showed a significant difference in the follicular recovery and survival rates among different enzymes and methods used. The maximal follicular yield was obtained by trypsin+EDTA and collagenase II digestion, followed by collagenase type IA digestion. However, the highest follicular viability rate was observed in groups of collagenase type IA digestion and the mechanical isolation method. Enzymatic treatment resulted in higher misshapen oocytes or follicles, though the diameters of the follicles were not significantly changed. In addition, our follicle culture results for different conditions showed maximal survival rates of primordial follicles in alginate hydrogel beads after 12 d of culture. Thus, we successfully established methods for isolating and culturing chicken primordial follicles. The present method will greatly facilitate investigation of the regulation of follicular development. © 2015 Poultry Science Association Inc.

  10. Organisational culture: variation across hospitals and connection to patient safety climate.

    Science.gov (United States)

    Speroff, T; Nwosu, S; Greevy, R; Weinger, M B; Talbot, T R; Wall, R J; Deshpande, J K; France, D J; Ely, E W; Burgess, H; Englebright, J; Williams, M V; Dittus, R S

    2010-12-01

    Bureaucratic organisational culture is less favourable to quality improvement, whereas organisations with group (teamwork) culture are better aligned for quality improvement. To determine if an organisational group culture shows better alignment with patient safety climate. Cross-sectional administration of questionnaires. Setting 40 Hospital Corporation of America hospitals. 1406 nurses, ancillary staff, allied staff and physicians. Competing Values Measure of Organisational Culture, Safety Attitudes Questionnaire (SAQ), Safety Climate Survey (SCSc) and Information and Analysis (IA). The Cronbach alpha was 0.81 for the group culture scale and 0.72 for the hierarchical culture scale. Group culture was positively correlated with SAQ and its subscales (from correlation coefficient r = 0.44 to 0.55, except situational recognition), ScSc (r = 0.47) and IA (r = 0.33). Hierarchical culture was negatively correlated with the SAQ scales, SCSc and IA. Among the 40 hospitals, 37.5% had a hierarchical dominant culture, 37.5% a dominant group culture and 25% a balanced culture. Group culture hospitals had significantly higher safety climate scores than hierarchical culture hospitals. The magnitude of these relationships was not affected after adjusting for provider job type and hospital characteristics. Hospitals vary in organisational culture, and the type of culture relates to the safety climate within the hospital. In combination with prior studies, these results suggest that a healthcare organisation's culture is a critical factor in the development of its patient safety climate and in the successful implementation of quality improvement initiatives.

  11. Southern Federal University in Relation to Teacher Education Modernization Project: Strategic Reflection of the Testing Results of Integrative Modules of the “Cultural-Historical Psychology and Activity Approach in Education” Research Master’s Program

    Directory of Open Access Journals (Sweden)

    Chernaya A. V.

    2015-11-01

    Full Text Available The article describes the experience of a strategic reflection of the Southern Federal University participation in the project of modernization of teacher education. It analyses how theoretical, methodological and organizational-activity basis of “Cultural-Historical Psychology and Activity Approach in Education” research master’s program designed by Moscow State University of Psychology & Education relates to the strategic objectives of Southern Federal University. Priorities and forward-looking statements in the strategy for the training of specialists in education based on action-competence approach, integrative principle of educational modules construction, network forms of cooperation of educational institutions are shown. Basic methodological ideas of culturalhistorical psychology and activity theory and their applicability to the needs of modern education objectified in the draft are specially considered. The article presents the preliminary test results of integrative modules of research master’s program for Southern Federal University

  12. Skin or nail culture

    Science.gov (United States)

    Mucosal culture; Culture - skin; Culture - mucosal; Nail culture; Culture - fingernail; Fingernail culture ... There, it is placed in a special dish (culture). It is then watched to see if bacteria, ...

  13. Formal Safety versus Real Safety: Quantitative and Qualitative Approaches to Safety Culture – Evidence from Estonia

    Directory of Open Access Journals (Sweden)

    Järvis Marina

    2016-10-01

    Full Text Available This paper examines differences between formal safety and real safety in Estonian small and medium-sized enterprises. The results reveal key issues in safety culture assessment. Statistical analysis of safety culture questionnaires showed many organisations with an outstanding safety culture and positive safety attitudes. However, qualitative data indicated some important safety weaknesses and aspects that should be included in the process of evaluation of safety culture in organisations.

  14. 探讨血培养阳性结果在新生儿血流感染中的意义%Significance of blood culture positive results in judging neonatal bloodstream infections

    Institute of Scientific and Technical Information of China (English)

    张斗星; 张林; 李宗光; 胡安群

    2012-01-01

    目的 探讨新生儿血培养阳性的临床意义.方法 对2011年1 月至2011年12月科住院患儿血培养标本中分离到的阳性结果116 例进行分析,区别致病菌和污染菌.结果 116 例血培养阳性结果中,主要为革兰阳性球菌82 例(70.7%),革兰阴性杆菌33例(28.4%),白色念珠菌1 例(0.9%),判断为血流感染为52 例(44.8%),污染菌为64 例(55.2%),污染菌主要以血浆凝固酶阴性葡萄球菌为主;MRSA、MRCNS 和产ESBLs 细菌在致病菌和污染菌检出率基本一致,耐药谱基本相同;致病菌的阳性报警时间明显少于污染菌报警时间.结论 新生儿血培养报警阳性中,污染率较高,以血浆凝固酶阴性葡萄球菌为主,医师需将实验室结果与临床资料结合考虑,正确区分污染菌,发挥血培养的作用.%Objective To explore the clinical significance of the positive blood culture results in neonates. Methods A retrospective analysis of positive blood culture from 116 neonatal hospitalized patients in Anqing Municipal Hospital from January 2011 to December 2011 was done. Strains of pathogen and bacterium were identified. Results Eighty-two of 116 strains were from patients with gram-positive bacteria, 33 strains with gram negative bacilli, 1 strain with Candida albicans; fifty-two of 116 strains from patients with bloodstream infection and the other 64 strains with bacterium, mainly coagula.se negative staphylococcus (CNS). The detection rales of MRSA, MRCNS and KSBI.s from pathogen and bacterium were basically the same and the drug resistance was also similar. The mean growth time of pathogen was significantly shorter than that oi bacterium. Conclusion The incidence of coagulase negative staphylococcus bacterium in neonatal positive blood culture was relatively high. It is important for doctors to combine laboratory results with clinical materials to indentify bacterium and play the role of blood culture.

  15. Modeling Cultural Dynamics

    CERN Document Server

    Gabora, Liane

    2008-01-01

    EVOC (for EVOlution of Culture) is a computer model of culture that enables us to investigate how various factors such as barriers to cultural diffusion, the presence and choice of leaders, or changes in the ratio of innovation to imitation affect the diversity and effectiveness of ideas. It consists of neural network based agents that invent ideas for actions, and imitate neighbors' actions. The model is based on a theory of culture according to which what evolves through culture is not memes or artifacts, but the internal models of the world that give rise to them, and they evolve not through a Darwinian process of competitive exclusion but a Lamarckian process involving exchange of innovation protocols. EVOC shows an increase in mean fitness of actions over time, and an increase and then decrease in the diversity of actions. Diversity of actions is positively correlated with population size and density, and with barriers between populations. Slowly eroding borders increase fitness without sacrificing diver...

  16. This research is to study the factors which influence the business success of small business ‘processed rotan’. The data employed in the study are primary data within the period of July to August 2013, 30 research observations through census method. Method of analysis used in the study is multiple linear regressions. The results of analysis showed that the factors of labor, innovation and promotion have positive and significant influence on the business success of small business ‘processed rotan’ simultaneously. The analysis also showed that partially labor has positive and significant influence on the business success, yet innovation and promotion have insignificant and positive influence on the business success.

    OpenAIRE

    Nasution, Inggrita Gusti Sari; Muchtar, Yasmin Chairunnisa

    2013-01-01

    This research is to study the factors which influence the business success of small business ‘processed rotan’. The data employed in the study are primary data within the period of July to August 2013, 30 research observations through census method. Method of analysis used in the study is multiple linear regressions. The results of analysis showed that the factors of labor, innovation and promotion have positive and significant influence on the business success of small busine...

  17. Should we learn culture in chemistry classroom? Integration ethnochemistry in culturally responsive teaching

    Science.gov (United States)

    Rahmawati, Yuli; Ridwan, Achmad; Nurbaity

    2017-08-01

    The papers report the first year of two-year longitudinal study of ethnochemistry integration in culturally responsive teaching in chemistry classrooms. The teaching approach is focusing on exploring the culture and indigenous knowledge in Indonesia from chemistry perspectives. Ethnochemistry looks at the culture from chemistry perspectives integrated into culturally responsive teaching has developed students' cultural identity and students' engagement in chemistry learning. There are limited research and data in exploring Indonesia culture, which has around 300 ethics, from chemistry perspectives. Students come to the chemistry classrooms from a different background; however, their chemistry learning disconnected with their background which leads to students' disengagement in chemistry learning. Therefore this approach focused on students' engagement within their differences. This research was conducted with year 10 and 11 from four classrooms in two secondary schools through qualitative methodology with observation, interviews, and reflective journals as data collection. The results showed that the integration of ethnochemistry in culturally responsive teaching approach can be implemented by involving 5 principles which are content integration, facilitating knowledge construction, prejudice reduction, social justice, and academic development. The culturally responsive teaching has engaged students in their chemistry learning and developed their cultural identity and soft skills. Students found that the learning experiences has helped to develop their chemistry knowledge and understand the culture from chemistry perspectives. The students developed the ability to work together, responsibility, curiosity, social awareness, creativity, empathy communication, and self-confidence which categorized into collaboration skills, student engagement, social and cultural awareness, and high order thinking skills. The ethnochemistry has helped them to develop the critical self

  18. Culturing Schwann Cells from Neonatal Rats by Improved Enzyme Digestion Combined with Explants-culture Method.

    Science.gov (United States)

    Liu, Di; Liang, Xiao-Chun; Zhang, Hong

    2016-08-01

    Objective To develop an improved method for culturing Schwann cells(SCs) by using both enzyme digestion and explants-culture approaches and compared with traditional explants-culture method and general hemi-explants-culture method. Methods Bilaterally sciatic nerves and brachial plexus nerves were dissected from 3 to 5-day-old neonatal SD rats and explants-culture method,general hemi-explants-culture method,and improved enzyme digestion combined with explants-culture method were adopted to culture SCs,respectively. SCs were digested and passaged after 7 days in culture and counted under the microscope. The purity of SCs was identified by S-100 immunofluorescence staining. Results The SCs of improved method group grew fastest and the total number of cells obtained was(1.85±0.13)×10(6);the SCs of the hemi-explants-culture method group grew slower than the improved method group and the total number of cells obtained was (1.10±0.10)×10(6);the SCs of the explants-culture method group grew slowest and the total number of cells obtained was (0.77±0.03)×10(6).The total number of cells obtained showed significant difference among the three groups(Pculture method group,and (74.50±4.23)% in the explants-culture method group(Pculture method can obtain sufficient amount of high-purity SCs in a short time and thus may be applied in further research on peripheral nerve regeneration.

  19. Biomimetic Multispiked Connecting Ti-Alloy Scaffold Prototype for Entirely-Cementless Resurfacing Arthroplasty Endoprostheses—Exemplary Results of Implantation of the Ca-P Surface-Modified Scaffold Prototypes in Animal Model and Osteoblast Culture Evaluation

    Directory of Open Access Journals (Sweden)

    Ryszard Uklejewski

    2016-06-01

    Full Text Available We present here—designed, manufactured, and tested by our research team—the Ti-alloy prototype of the multispiked connecting scaffold (MSC-Scaffold interfacing the components of resurfacing arthroplasty (RA endoprostheses with bone. The spikes of the MSC-Scaffold prototype mimic the interdigitations of the articular subchondral bone, which is the natural biostructure interfacing the articular cartilage with the periarticular trabecular bone. To enhance the osteoinduction/osteointegration potential of the MSC-Scaffold, the attempts to modify its bone contacting surfaces by the process of electrochemical cathodic deposition of Ca-P was performed with further immersion of the MSC-Scaffold prototypes in SBF in order to transform the amorphous calcium-phosphate coating in hydroxyapatite-like (HA-like coating. The pilot experimental study of biointegration of unmodified and Ca-P surface-modified MSC-Scaffold prototypes was conducted in an animal model (swine and in osteoblast cell culture. On the basis of a microscope-histological method the biointegration was proven by the presence of trabeculae in the interspike spaces of the MSC-Scaffold prototype on longitudinal and cross-sections of bone-implant specimens. The percentage of trabeculae in the area between the spikes of specimen containing Ca-P surface modified scaffold prototype observed in microCT reconstructions of the explanted joints was visibly higher than in the case of unmodified MSC-Scaffold prototypes. Significantly higher Alkaline Phosphatase (ALP activity and the cellular proliferation in the case of Ca-P-modified MSC-Scaffold pre-prototypes, in comparison with unmodified pre-prototypes, was found in osteoblast cell cultures. The obtained results of experimental implantation in an animal model and osteoblast cell culture evaluations of Ca-P surface-modified and non-modified biomimetic MSC-Scaffold prototypes for biomimetic entirely-cementless RA endoprostheses indicate the

  20. Cultural probes

    DEFF Research Database (Denmark)

    Madsen, Jacob Østergaard

    2016-01-01

    The aim of this study was thus to explore cultural probes (Gaver, Boucher et al. 2004), as a possible methodical approach, supporting knowledge production on situated and contextual aspects of occupation.......The aim of this study was thus to explore cultural probes (Gaver, Boucher et al. 2004), as a possible methodical approach, supporting knowledge production on situated and contextual aspects of occupation....

  1. Manuscript Cultures

    DEFF Research Database (Denmark)

    What do Mesoamerica, Greece, Byzantium, Island, Chad, Ethiopia, India, Tibet, China and Japan have in common? Like many other cultures of the world, they share a particular form of cultural heritage: ancient handwritten documents. In 2007, scholars from some20 countries around the world gathered...

  2. Culture Stories

    DEFF Research Database (Denmark)

    Jensen, Ole B.

    2007-01-01

    by certain representations and embedded in certain norms and values. The analytical framework is applied on a case of cultural urban branding. The case is the harbour front in Aalborg, Denmark where a number of flagship architecture projects and cultural institutions are being planned. It is shown how...

  3. Cultural Communications.

    Science.gov (United States)

    Armas, Jose

    It is too often taken for granted that the communication process with culturally different children takes place as readily as it might with children from Anglo cultures. Most teachers receive training in verbal and formal communication skills; children come to school with nonverbal and informal communication skills. This initially can create…

  4. Optimization and radiation protection culture

    Energy Technology Data Exchange (ETDEWEB)

    Jeon, In Young; Shin, Hyeong Ki; Lee, Chan Mi [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)

    2013-04-15

    Safety culture or radiation protection culture is based in common on the term, 'culture'. Culture is defined as the learned, shared set of symbols and patterns of basic assumptions, which is invented, discovered, or developed by a given group as it learns to cope with its problem of external adaptation and internal integration. Safety culture generally refers to the attitude and behaviors affecting safety performance. The concept of 'Safety Culture' was introduced after the Chernobyl accident in 1986. For the accident, nuclear society reached the conclusion that the cause was the wrong management attitude of the NPP, that is, deficient 'Safety Culture'. Recently, 'Radiation Protection Culture' was introduced as the core concept of nuclear safety culture. There have been many efforts to establish definition and develop assessment tool for radiation protection culture in international level such as ICRP and IRPA as well as NRC. In the same context with the safety culture, radiation protection culture is defined as 'the core values and behaviors resulting from a collective commitment by leaders and individual's to emphasize safety over competing goals to ensure protection of people and the environment.' It is worthwhile to recognize that regulatory enforcement in establishing healthy radiation protection culture of operators should be minimized because culture is not in the domain of regulatory enforcement. However, as 'ALARA', the most important concept in radiation protection, may be successfully achieved only in well established radiation protection culture, the least regulatory intervention would be needed in promoting and nurturing radiation protection culture in licensee. In addition, the concept of radiation protection culture should be addressed in plant operational policy to achieve the goals of ALARA. The pre-condition of the successful radiation protection culture is a healthy organizational

  5. Distinction or Omnivorousness? -- An Analysis of Cultural Participation and Class Reproduction in Kaohsiung from the Perspective of Pierre Bourdieu

    Directory of Open Access Journals (Sweden)

    Chih-Hsien Chen

    2016-01-01

    Full Text Available Focusing upon cultural politics of class relations, this study uses the case of Kaohsiung to explore into four major research questions: How do people participate in cultural activities? What are the possible factors that have influenced cultural taste? What are significant variables related to the quality and quantity of cultural participation? And to what extent there do exist distinction and class reproduction? Data have been collected through a citywide telephone random survey and analyzed from the perspective of Pierre Bourdieu to sort out the causes and consequences of cultural participation. Statistic results show that music, movie and animation are the most popular cultural activities and they are regarded as the very cultural industries that should be fully promoted by the government as well. Cultural capital, according to regression analyses, determines the quality and quantity of cultural participation, whereas cultural participation has further demarcated the difference of class identities, reproduced existing cultural advantage, and facilitated the exchange between cultural capital and social capital. Overall speaking, the composition and volume of cultural capital and economic capital are related to the quality of cultural participation. The volume of cultural capital has positive correlation with the quantity of cultural participation. Distinction, thus, works with cultural omnivorousness side by side, and the outcomes of cultural participation in return have led to certain kinds of class identification and class reproduction.

  6. CULTURAL TOURISM

    Directory of Open Access Journals (Sweden)

    Dana POP

    2016-07-01

    Full Text Available In this paper we will try to analyse the cultural tourism. We will start by referring to the complex concepts of tourism and culture and to the synergies existing between them. We will define cultural tourism and present its appearance and evolution as well as its importance as a modern form of tourism. We will present the various types of cultural tourism with their characteristics and the specific features of cultural tourists according to their interests. We will also mention that there are advantages and disadvantages for any kind of tourism depending on the position – local communities, companies or tourists. For the future we will refer to the new partnership between UNWTO and UNESCO.

  7. Cultural hegemony? Educators’ perspectives on facilitating cross-cultural dialogue

    Science.gov (United States)

    Zaidi, Zareen; Verstegen, Daniëlle; Vyas, Rashmi; Hamed, Omayma; Dornan, Tim; Morahan, Page

    2016-01-01

    Background We live in an age when education is being internationalized. This can confront students with ‘cultural hegemony’ that can result from the unequal distribution of power and privilege in global society. The name that is given to awareness of social inequality is ‘critical consciousness’. Cross-cultural dialogue provides an opportunity for learners to develop critical consciousness to counter cultural hegemony. The purpose of this research was to understand how learners engage with cross-cultural dialogue, so we can help them do so more effectively in the future. Method The setting for this research was an online discussion in an international health professions educator fellowship program. We introduced scenarios with cultural references to study the reaction of participants to cultural conversation cues. We used an inductive thematic analysis to explore power and hegemony issues. Results Participants reflected that personally they were more likely to take part in cross-cultural discussions if they recognized the context discussed or had prior exposure to educational settings with cultural diversity. They identified barriers as lack of skills in facilitating cross-cultural discussions and fear of offending others. They suggested deliberately introducing cultural issues throughout the curriculum. Conclusion Our results indicate that developing critical consciousness and cross-cultural competency will require instructional design to identify longitudinal opportunities to bring up cross-cultural issues, and training facilitators to foster cross-cultural discussions by asking clarifying questions and navigating crucial/sensitive conversations. PMID:27890048

  8. Cultural hegemony? Educators’ perspectives on facilitating cross-cultural dialogue

    Directory of Open Access Journals (Sweden)

    Zareen Zaidi

    2016-11-01

    Full Text Available Background: We live in an age when education is being internationalized. This can confront students with ‘cultural hegemony’ that can result from the unequal distribution of power and privilege in global society. The name that is given to awareness of social inequality is ‘critical consciousness’. Cross-cultural dialogue provides an opportunity for learners to develop critical consciousness to counter cultural hegemony. The purpose of this research was to understand how learners engage with cross-cultural dialogue, so we can help them do so more effectively in the future. Method: The setting for this research was an online discussion in an international health professions educator fellowship program. We introduced scenarios with cultural references to study the reaction of participants to cultural conversation cues. We used an inductive thematic analysis to explore power and hegemony issues. Results: Participants reflected that personally they were more likely to take part in cross-cultural discussions if they recognized the context discussed or had prior exposure to educational settings with cultural diversity. They identified barriers as lack of skills in facilitating cross-cultural discussions and fear of offending others. They suggested deliberately introducing cultural issues throughout the curriculum. Conclusion: Our results indicate that developing critical consciousness and cross-cultural competency will require instructional design to identify longitudinal opportunities to bring up cross-cultural issues, and training facilitators to foster cross-cultural discussions by asking clarifying questions and navigating crucial/sensitive conversations.

  9. Culture growth of testate amoebae under different silicon concentrations.

    Science.gov (United States)

    Wanner, Manfred; Seidl-Lampa, Barbara; Höhn, Axel; Puppe, Daniel; Meisterfeld, Ralf; Sommer, Michael

    2016-10-01

    Testate amoebae with self-secreted siliceous shell platelets ("idiosomes") play an important role in terrestrial silicon (Si) cycles. In this context, Si-dependent culture growth dynamics of idiosomic testate amoebae are of interest. Clonal cultures of idiosomic testate amoebae were analyzed under three different Si concentrations: low (50μmolL(-1)), moderate/site-specific (150μmolL(-1)) and high Si supply (500μmolL(-1)). Food (Saccharomyces cerevisiae) was provided in surplus. (i) Shell size of four different clones of idiosomic testate amoebae either decreased (Trinema galeata, Euglypha filifera cf.), increased (E. rotunda cf.), or did not change (E. rotunda) under the lowest Si concentration (50μmolSiL(-1)). (ii) Culture growth of idiosomic Euglypha rotunda was dependent on Si concentration. The more Si available in the culture medium, the earlier the entry into exponential growth phase. (iii) Culture growth of idiosomic Euglypha rotunda was dependent on origin of inoculum. Amoebae previously cultured under a moderate Si concentration revealed highest sustainability in consecutive cultures. Amoebae derived from cultures with high Si concentrations showed rapid culture growth which finished early in consecutive cultures. (iv) Si (diluted in the culture medium) was absorbed by amoebae and fixed in the amoeba shells resulting in decreased Si concentrations.

  10. 立足“高等性”、凸显“职业性”是高职院校校园文化建设的核心--以顺德职业技术学院为例%Based on the"Higher Attribute",Showing the"Vocational Attribute"the Core of the Construction of Campus Culture in Higher Vocational Colleges---Taking Shunde Polytechnic as an example

    Institute of Scientific and Technical Information of China (English)

    强金国; 胡蓉

    2014-01-01

    Higher vocational campus culture construction possesses " higher attribute " and " vocational attribute ", during its construction, we should keep a foothold on " higher attribute ",promote its gradation and taste, show " vocational attribute ", highlight its role and target, which aims to construct favorable atmosphere for cultivating higher quality skilled personnel. In this aspect, Shunde Polytechnic carried out precious exploration and practice.%高职校园文化兼具高等性和职业性特征。建设中,要立足“高等性”,提升其层次和品位;凸显“职业性”,突出其角色和面向,为培养高素质技能型人才营造良好氛围。这方面,顺德职院开展了可贵的探索和实践。

  11. Astronomy and culture

    CERN Document Server

    Hetherington, Edith

    2009-01-01

    While astronomy is a burgeoning science, with tremendous increases in knowledge every year, it also has a tremendous past, one that has altered humanity's understanding of our place in the universe. The impact of astronomy on culture - whether through myths and stories, or through challenges to the intellectual status quo - is incalculable. This volume in the Greenwood Guides to the Universe series examines how human cultures, in all regions and time periods, have tried to make sense of the wonders of the universe. Astronomy and Culture shows students how people throughout time have struggled

  12. Organizational Culture and Industrialization

    DEFF Research Database (Denmark)

    Kragh, Simon Ulrik

    Drawing on a revised version ofHofstede's theory ofindustrialization and cultural change contained in his explanation of individualism and collectivism, the paper proposes that countries which are in the earlier stages of industrialization have a common culture that governs organizational...... behaviours. In-group/out-group particularist values that have been handed over from preindustrial society tend to overlay and replace impersonal and universalistic bureaucracies and market exchange typical ofindustrial society. The paper shows how these values shape the culture of organizations in Latin...... America, Africa and Germany around 1850....

  13. Bilingual Cultural Differences and Communication

    Institute of Scientific and Technical Information of China (English)

    ZHANG Fu-sheng; HUA Qing-liang

    2001-01-01

    For historical, regional or other reasons, there are some great differences between the Chinese and the English culture. Generally, the keynote of the western culture is the superiority of lust, while the Chinese culture stresses on reservation and compromise. The westerners emphasize individualism, while the Chinese lay stress on context. Different cultures contribute to different habits, characters and behaviors as well as different registers.Cultural differences can directly lead to information loss, information misleading, thus result in obstacles in communication. This essay will have a detailed analysis on the cultural differences and their influence on communication.

  14. a Cultural Market Model

    Science.gov (United States)

    HerdaǦDELEN, Amaç; Bingol, Haluk

    Social interactions and personal tastes shape our consumption behavior of cultural products. In this study, we present a computational model of a cultural market and we aim to analyze the behavior of the consumer population as an emergent phenomena. Our results suggest that the final market shares of cultural products dramatically depend on consumer heterogeneity and social interaction pressure. Furthermore, the relation between the resulting market shares and social interaction is robust with respect to a wide range of variation in the parameter values and the type of topology.

  15. A Cultural Market Model

    CERN Document Server

    Herdagdelen, Amac

    2008-01-01

    Social interactions and personal tastes shape our consumption behavior of cultural products. In this study, we present a computational model of a cultural market and we aim to analyze the behavior of the consumer population as an emergent phenomena. Our results suggest that the final market shares of cultural products dramatically depend on consumer heterogeneity and social interaction pressure. Furthermore, the relation between the resulting market shares and social interaction is robust with respect to a wide range of variation in the parameter values and the type of topology.

  16. Cultural History and Cultural Materialism.

    Science.gov (United States)

    Berman, Ronald

    1990-01-01

    Historicism critiques cultural history and cultural materialism as a methodology for literary analysis. Questions the finality of interpretation, how original values change, and whether dramatic history implies actual history. Using Shakespearean plays, analyzes the power and politics of a play in relation to its audience; posits that cultural…

  17. Cultural History and Cultural Materialism.

    Science.gov (United States)

    Berman, Ronald

    1990-01-01

    Historicism critiques cultural history and cultural materialism as a methodology for literary analysis. Questions the finality of interpretation, how original values change, and whether dramatic history implies actual history. Using Shakespearean plays, analyzes the power and politics of a play in relation to its audience; posits that cultural…

  18. Digging culture and doing culture

    NARCIS (Netherlands)

    Andries van den Broek; Jos de Haan; Frank Huysmans

    2009-01-01

    Original title: Cultuurbewonderaars en cultuurbeoefenaars. There are people who love art and culture and there are people who practise it; people who enjoy it and people who are themselves creative in their leisure time. Who are these culture-lovers and practitioners? How has participation in

  19. Reactive glia show increased immunoproteasome activity in Alzheimer's disease.

    Science.gov (United States)

    Orre, Marie; Kamphuis, Willem; Dooves, Stephanie; Kooijman, Lieneke; Chan, Elena T; Kirk, Christopher J; Dimayuga Smith, Vanessa; Koot, Sanne; Mamber, Carlyn; Jansen, Anne H; Ovaa, Huib; Hol, Elly M

    2013-05-01

    The proteasome is the major protein degradation system within the cell, comprised of different proteolytic subunits; amyloid-β is thought to impair its activity in Alzheimer's disease. Neuroinflammation is a prominent hallmark of Alzheimer's disease, which may implicate an activation of the immunoproteasome, a specific proteasome variant induced by immune signalling that holds slightly different proteolytic properties than the constitutive proteasome. Using a novel cell-permeable proteasome activity probe, we found that amyloid-β enhances proteasome activity in glial and neuronal cultures. Additionally, using a subunit-specific proteasome activity assay we showed that in the cortex of the APPswePS1dE9 plaque pathology mouse model, immunoproteasome activities were strongly increased together with increased messenger RNA and protein expression in reactive glia surrounding plaques. Importantly, this elevated activity was confirmed in human post-mortem tissue from donors with Alzheimer's disease. These findings are in contrast with earlier studies, which reported impairment of proteasome activity in human Alzheimer's disease tissue and mouse models. Targeting the increased immunoproteasome activity with a specific inhibitor resulted in a decreased expression of inflammatory markers in ex vivo microglia. This may serve as a potential novel approach to modulate sustained neuroinflammation and glial dysfunction associated with Alzheimer's disease.

  20. Cultural change that sticks.

    Science.gov (United States)

    Katzenbach, Jon R; Steffen, Ilona; Kronley, Caroline

    2012-01-01

    When a major change initiative runs aground, leaders often blame their company's culture for pushing it off course. They try to forge ahead by overhauling the culture--a tactic that tends to fizzle, fail, or backfire. Most cultures are too well entrenched to be jettisoned. The secret is to stop fighting your culture--and to work with and within it, until it evolves in the right direction. Today's best-performing companies, such as Southwest Airlines, Apple, and the Four Seasons, understand this, say the authors, three consultants from Booz & Company. These organizations follow five principles for making the most of their cultures: 1. Match strategy to culture. Culture trumps strategy every time, no matter how brilliant the plan, so the two need to be in alignment. 2. Focus on a few critical shifts in behavior. Wholesale change is hard; choose your battles wisely. 3. Honor the strengths of the existing culture. Every culture is the product of good intentions and has strengths; put them to use. 4. Integrate formal and informal interventions. Don't just implement new rules and processes; identify "influencers" who can bring other employees along. 5. Measure and monitor cultural evolution. Otherwise you can't identify backsliding or correct course. When the leaders of Aetna applied these rules while implementing a new strategy in the early 2000s, they reinvigorated the company's ailing culture and restored employee pride. That shift was reflected in the business results, as Aetna went from a $300 million loss to a $1.7 billion gain.

  1. Culture and body image

    Directory of Open Access Journals (Sweden)

    D. Alves

    2009-01-01

    Full Text Available The present study aims to investigate the relationship between culture and body image. We intend to know how socio-cultural factors influence the levels of satisfaction with body image. The emphasis is given to the cultural values as represented by the sociocultural norms of societies such as the United States of America and Europe. It is argued that through the media, the values of these industrialized societies are dissipated throughout the world provoking cultural changes and uniformization of behavioural standards. From the literature review, it is possible to conclude that body dissatisfaction is a reality to both sexes and a direct result of the non-conformity to cultural-esthetical patterns promoted by the profit-oriented societies.

  2. Culture and cooperation.

    Science.gov (United States)

    Gächter, Simon; Herrmann, Benedikt; Thöni, Christian

    2010-09-12

    Does the cultural background influence the success with which genetically unrelated individuals cooperate in social dilemma situations? In this paper, we provide an answer by analysing the data of Herrmann et al. (2008a), who studied cooperation and punishment in 16 subject pools from six different world cultures (as classified by Inglehart & Baker (2000)). We use analysis of variance to disentangle the importance of cultural background relative to individual heterogeneity and group-level differences in cooperation. We find that culture has a substantial influence on the extent of cooperation, in addition to individual heterogeneity and group-level differences identified by previous research. The significance of this result is that cultural background has a substantial influence on cooperation in otherwise identical environments. This is particularly true in the presence of punishment opportunities.

  3. Impact of seawater pCO2 changes on calcification and on Mg/Ca and Sr/Ca in benthic foraminifera calcite (Ammonia tepida: results from culturing experiments

    Directory of Open Access Journals (Sweden)

    J. Bijma

    2009-04-01

    Full Text Available Evidence is accumulating of increasing concentrations of dissolved carbon dioxide in the ocean and associated acidification impacts on calcifying organisms. Among these organisms, benthic and planktonic foraminifera are responsible for a large amount of the globally precipitated calcium carbonate. Therefore, their response to an acidifying ocean may have important consequences for future inorganic carbon cycling. To assess the sensitivity of benthic foraminifera to changing carbon dioxide levels and subsequent alteration in seawater carbonate chemistry, we cultured specimens of the shallow water species Ammonia tepida at two concentrations of atmospheric CO2 (120 and 2000 ppm and two temperatures (10°C and 15°C. Shell weights and elemental compositions were determined. Results indicate that shell weights decrease with decreasing [CO32−], and increase with decreasing temperature. Changes in [CO32−] or total dissolved inorganic carbon do not affect the Mg partition coefficient. On the contrary, Sr incorporation is enhanced under increasing [CO32−]. Implications of these results for the paleoceanographic application of foraminifera are also discussed.

  4. The Notion of Culture in Linguistic Research

    Directory of Open Access Journals (Sweden)

    Dominic Busch

    2009-01-01

    Full Text Available Many works on intercultural communication from the field of linguistics share the assumption that influences of culture on social interaction will manifest in communicative exchanges—and conversely, that an academic's look at these exchanges will be a sufficient basis for an adequate description of what intercultural communication is supposed to be about. Linguistic theory itself lacking of places to integrate culture as a factor into its concepts, urges scholars to borrow operationalizations of culture from neighboring disciplines like e.g. different strands of psychology, sociology or anthropology. Approaches resulting from this transdisciplinary orientation as a consequence share very divergent assumptions on how, at what moment in a communicative process and with what effects culture influences social interaction. While many surveys on similar behalf distinguish between primordial and constructionist approaches, a closer look at different strands of empirical linguistic research may reveal even more precise and detailed distinctions on how culture may be captured and framed. This article will present and analyze a selection of approaches from the mentioned field, e.g. from intercultural and contrastive pragmatics, interactional sociolinguistics, ethnography of communication, ethnomethodology as well as discourse analysis. In each case, the underlying notions of culture will be revealed and put into contrast. Additionally, this exemplary analysis may show that most of the empirical schools mentioned follow and adopt changing notions of culture from social theory over time. URN: urn:nbn:de:0114-fqs0901508

  5. Dynamic culture improves cell reprogramming efficiency.

    Science.gov (United States)

    Sia, Junren; Sun, Raymond; Chu, Julia; Li, Song

    2016-06-01

    Cell reprogramming to pluripotency is an inefficient process and various approaches have been devised to improve the yield of induced pluripotent stem cells. However, the effect of biophysical factors on cell reprogramming is not well understood. Here we showed that, for the first time, dynamic culture with orbital shaking significantly improved the reprogramming efficiency in adherent cells. Manipulating the viscosity of the culture medium suggested that the improved efficiency is mainly attributed to convective mixing rather than hydrodynamic shear stress. Temporal studies demonstrated that the enhancement of reprogramming efficiency required the dynamic culture in the middle but not early phase. In the early phase, fibroblasts had a high proliferation rate, but as the culture became over-confluent in the middle phase, expression of p57 was upregulated to inhibit cell proliferation and consequently, cell reprogramming. Subjecting the over confluent culture to orbital shaking prevented the upregulation of p57, thus improving reprogramming efficiency. Seeding cells at low densities to avoid over-confluency resulted in a lower efficiency, and optimal reprogramming efficiency was attained at a high seeding density with dynamic culture. Our findings provide insight into the underlying mechanisms of how dynamic culture condition regulate cell reprogramming, and will have broad impact on cell engineering for regenerative medicine and disease modeling.

  6. Clinical study of results of sputum culture for elderly patients with severe infections before and after treatment%重症感染老年患者治疗前后痰培养结果的临床研究

    Institute of Scientific and Technical Information of China (English)

    方明; 陈霞; 龚旭红

    2015-01-01

    目的:探讨内分泌科重症感染老年患者抗感染治疗前后痰培养结果的改变以及影响患者预后的因素,以降低感染率。方法选取内分泌科收治的重症感染老年患者87例,于治疗前以及治疗后每周进行1次痰培养,记录培养结果;在患者治疗4周后,根据治疗效果将其分为有效组58例和无效组29例,对两组患者的临床资料进行统计,分析影响治疗效果的因素。结果经治疗,继续接受治疗患者逐渐减少,但痰培养细菌阳性率逐渐升高,在第4周,仍然有36例患者接受治疗,这些患者痰培养全部为阳性;治疗前共检出病原菌66株,其中革兰阴性菌51株占77.27%,革兰阳性菌10株占15.15%,真菌4株占6.06%;随着治疗时间的推移,接受治疗患者的病原菌谱无显著性变化;随着治疗的进行,菌株清除率逐渐升高,但是也不断的有新菌株定植;无效或死亡患者中肾功能不全者明显多于显效有效患者,两组比较差异有统计学意义(P<0.05)。结论铜绿假单胞菌是内分泌科重症感染老年患者常见病原菌,且随着治疗患者的细菌菌谱也随之变化,在临床治疗过程中应根据药敏试验选择敏感抗菌药物的同时适当应用广谱抗菌药物,同时主要保护患者的肾功能。%OBJECTIVE To explore the change of results of sputum culture for the elderly patients with severe in‐fections in endocrinology department before and after the anti‐infection therapy and observe the influencing factors for clinical outcomes so as to reduce the infection rate .METHODS A total of 87 elderly patients with severe infec‐tions who were treated in the endocrinology department were enrolled in the study ,then the sputum culture was performed before the treatment and once per week after the treatment ,and the culture results were recorded ;at week 4 of treatment ,the patients were divided into the

  7. Interpretation of California mastitis test scores using Staphylococcus aureus culture results for screening of subclinical mastitis in low yielding smallholder dairy cows in the Dar es Salaam region of Tanzania.

    Science.gov (United States)

    Kivaria, F M; Noordhuizen, J P T M; Nielen, M

    2007-03-17

    Screening of subclinical mastitis under field conditions is done using the California mastitis test (CMT). CMT score of > or = 1 corresponding to > or = 500,000 somatic cells ml(-1) is commonly used as threshold of subclinical mastitis in temperate countries. However, given the innately high physiological level of somatic cells in low yielding dairy cows, this threshold may not apply to low yielding dairy cows. The current study was undertaken to investigate the clinical utility of CMT for screening of Staphylococcus aureus subclinical mastitis in low yielding smallholder dairy cows in Tanzania. A total of 1151 of quarter-milk samples were CMT tested, of these 914-originated from cows with a lactation period of 14-305 days. All samples were screened for subclinical mastitis by the CMT as well as microbiological culture of single, duplicate (two consecutive) and triplicate (three consecutive) samples as a gold standard. For the duplicate and triplicate quarter-samples, cows were considered positive for S. aureus subclinical mastitis if results of microbiologic culture for S. aureus were positive for two of two, and for at least two of the first three consecutive quarter-milk samples collected from that cow, respectively. Using a CMT score of > or = 1 would classify 78.6% of the 940 quarter-samples as positive. Eighty-two percent of the samples in which S. aureus was isolated had CMT scores > or = 2; this would classify 51.6% of the 940 quarter-samples as positive. For the single sample, this cut-off had sensitivity, specificity and likelihood ratio for S. aureus of 0.87, 0.83 and 4.24, respectively. For the duplicate quarter-milk samples this cut-off had sensitivity, specificity, and likelihood ratio of 0.94, 0.86, and 5.19. While, for the triplicate quarter-milk samples this cut-off had sensitivity, specificity and likelihood ratio of 0.97, 0.92 and 7.47, respectively. Based on these results and practical considerations, it is concluded that CMT score of > or = 2

  8. Cultural diversity for virtual characters investigating behavioral aspects across cultures

    CERN Document Server

    Endrass, Birgit

    2014-01-01

    Culture plays a crucial role in our lives. Depending on our cultural background, we judge on and react to everything that we encounter. Subtle differences in behavior can lead to misunderstandings or even culture shock. In a similar manner, virtual characters can be declined by certain user groups when showing culturally inappropriate behavior. But how can social aspects such as culture be integrated into the behavioral models of virtual characters Birgit Endrass addresses this question by carrying out a hybrid approach that is based on theoretical background from the social sciences as well a

  9. Open Culture Data position paper

    NARCIS (Netherlands)

    L.B. Baltussen (Lotte); M. Brinkerink (Maarten); N. Timmermans (Nikki); M. Zeinstra (Maarten)

    2013-01-01

    textabstractOpen Culture Data started as a grassroots movement at the end of 2011, with the aim to open up data in the cultural sector and stimulate (creative) reuse. In this context, we organised a hackathon, which resulted in the creation of 13 Open Culture Data apps. After this successful first h

  10. Cultural Spectrum

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Exorbitant Performance Fees ProhibitedThe Ministry of Culture has taken measures todiscourage performance artists from charging excessively for their stage appearances. According to the ministry, payment for performers and ticket prices must be in line with average con-

  11. Rectal culture

    Science.gov (United States)

    ... 2016:chap 283. Haines CF, Sears CL. Infectious enteritis and proctocolitis. In: Feldman M, Friedman LS, Brandt ... PA: Elsevier; 2017:chap 22. Read More Cryptosporidium enteritis Fecal culture Proctitis Review Date 5/11/2016 ...

  12. Culture School

    Institute of Scientific and Technical Information of China (English)

    MATTHEW LIM

    2008-01-01

    @@ Until recently, employees posted abroad would, if they were lucky, receive some limited language training before they relocated. How they would cope with living in a completely new culture when they arrived was left up to them to figure out.

  13. Yangshao Culture

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Yangshao culture was born in the Neolithic Age. In 1921, archaeologists unearthed a number of chipped stone implements such as knives and axes; bone objects and everyday ceramic items. Thus Yangshao Village and its matriarchal society have

  14. Lemurs and macaques show similar numerical sensitivity

    Science.gov (United States)

    Jones, Sarah M.; Pearson, John; DeWind, Nicholas K.; Paulsen, David; Tenekedjieva, Ana-Maria; Brannon, Elizabeth M.

    2013-01-01

    We investigated the precision of the approximate number system (ANS) in three lemur species (Lemur catta, Eulemur mongoz, and Eulemur macaco flavifrons), one Old World monkey species (Macaca mulatta) and humans (Homo sapiens). In Experiment 1, four individuals of each nonhuman primate species were trained to select the numerically larger of two visual arrays on a touchscreen. We estimated numerical acuity by modeling Weber fractions (w) and found quantitatively equivalent performance among all four nonhuman primate species. In Experiment 2, we tested adult humans in a similar procedure, and they outperformed the four nonhuman species but showed qualitatively similar performance. These results indicate that the ANS is conserved over the primate order. PMID:24068469

  15. Ancient bacteria show evidence of DNA repair

    DEFF Research Database (Denmark)

    Johnson, Sarah Stewart; Hebsgaard, Martin B; Christensen, Torben R

    2007-01-01

    Recent claims of cultivable ancient bacteria within sealed environments highlight our limited understanding of the mechanisms behind long-term cell survival. It remains unclear how dormancy, a favored explanation for extended cellular persistence, can cope with spontaneous genomic decay over......-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence of bacterial survival in samples up to half a million years in age, making this the oldest independently authenticated DNA to date obtained from viable cells. Additionally, we find strong evidence...... that this long-term survival is closely tied to cellular metabolic activity and DNA repair that over time proves to be superior to dormancy as a mechanism in sustaining bacteria viability....

  16. Constructivism in cultural competence education.

    Science.gov (United States)

    Hunter, Jennifer L; Krantz, Steven

    2010-04-01

    A graduate course on cultural diversity, based in constructivist theory and structured on the Process of Cultural Competence in the Delivery of Healthcare Services model, was developed and taught through classroom and online methods. The following research questions were explored: 1) Can an educational experience, built on constructivist learning theory tenets, change students' perceptions, attitudes, knowledge, and skills in the area of cultural competence? 2) Does the delivery method, online or traditional classroom, influence the degree of change? The study used a quasi-experimental, pretest-posttest control group design using the Inventory for Assessing the Process of Cultural Competence Among healthcare Professionals Revised. Findings showed significant changes (p<0.001) in cultural competence scores and subscores for all learners with both teaching modalities based on interval scale and in categories of cultural knowledge, skills, desire, and overall competence based on a nominal scale. The untaught construct of cultural desire showed the most significant improvement.

  17. The Influence of Probiotics on Results of Common carp,Crucian Carp,and Grass Carp Culture%微生态制剂对鲤、鲫和草鱼养殖池塘效益的影响

    Institute of Scientific and Technical Information of China (English)

    李绍戊; 王荻; 尹家胜; 卢彤岩

    2011-01-01

    The effects of probiotics I containing primarily Bacillus subtilis and probiotics II containing a mixture of various microorganisms on weight gain,food conversion ratio and drug cost of common carp(Cyprinus carpio),Crucian carp(Carassius auratus) and grass carp(Ctenophyargodon idellus) in culture ponds in Heilongjiang and Liaoning provinces.The probiotics,alone or together,were showed to have influence on weight gain,the food conversion ratio and the drug cost in the fish culture.There were 423.57% higher weight gain in Heilongjiang province and 90% higher weight gain in Liaoning province in the ponds treated with the probiotics used together,than that in the control ponds with lower drug production cost of 29% in Heilongjiang province and 56.25% in Liaoning province.The food conversion ratio was found to be decreased by 0.35 and 0.05 in the ponds treated with probiotics used together,indicating that the probiotics have important influence on the profit of grass carp culture in Liaoning province and of common carp farming in Heilongjiang and have a promotion of green aquaculture with less drugs,less feed and more profits.%以枯草芽孢杆菌为主的主要用于促进鱼类消化生长的微生态制剂I、多种混合微生物主要用于调节水质的微生态制剂II,或是两者混合使用的方法,比较研究了黑龙江省及辽宁省池塘养殖的鲤(Cyprinus carpio)、鲫(Carassius auratus)和草鱼(Ctenophyargodon idellus)的增重率、饲料系数及药价等效益。结果表明,单种或者混合使用微生态制剂均对池塘养殖鲤及草鱼的增重率、饵料系数及用药价格有一定影响,同时使用两种微生态制剂提高鱼类增重率最显著,辽宁省和黑龙江省分别提高了423.57%和90%;而两省养殖池塘的药价也分别降低了29%和56.25%。微生态制剂I及混合同时使用两种微生态制剂均能显著降低饲料系数,辽宁及黑龙江分别降低0.35及0.05。结果可

  18. Culture perspectives.

    Science.gov (United States)

    Locsin, Rozzano C

    2002-10-01

    All cultures have had means and techniques that express their immediate aims. The thing that interests me is that today, painters do not have to go to a subject matter outside of themselves. They work from a different source. They work from within. It seems to me that the modern artist cannot express this age, the airplane, the atom bomb, the radio, in the old form of the Renaissance or of any of the old cultures.

  19. Cultural Imports

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    FOREIGN commodities have flowed into China since the country opened its doors to the outside world. China is an expansive territory with a huge population offering a vast potential consumer market. There are absolutely no limits to the world of culture in China, with Chinese people having access to foreign films, dramas, music and books, all of which have helped to strengthen exchanges between Chinese and Western cultures.

  20. Attitudes toward older adults: A matter of cultural values or personal values?

    Science.gov (United States)

    Zhang, Xin; Xing, Cai; Guan, Yanjun; Song, Xuan; Melloy, Robert; Wang, Fei; Jin, Xiaoyu

    2016-02-01

    The current research aimed to address the inconsistent findings regarding cultural differences in attitudes toward older adults by differentiating the effects of personal and cultural values. In Study 1, we used data from the sixth wave of the World Values Survey to examine attitudes toward older adults across cultures, and how different personal values (i.e., communal vs. agentic) and cultural values (i.e., individualism) predicted these attitudes. The results of hierarchical linear modeling analyses showed that after controlling for potential covariates, personal communal values positively correlated with positive attitudes toward older adults; however, cultural individualistic values did not. To further examine the causal effects of personal values (vs. cultural values), we conducted an experimental study and confirmed that priming personal values rather than cultural values had significant effects on ageism attitudes. The present studies help to reconcile conflicting results on cultural differences in attitudes toward older adults.

  1. Uncovering Metaethical Assumptions in Bioethical Discourse across Cultures.

    Science.gov (United States)

    Sullivan, Laura Specker

    2016-03-01

    Much of bioethical discourse now takes place across cultures. This does not mean that cross-cultural understanding has increased. Many cross-cultural bioethical discussions are marked by entrenched disagreement about whether and why local practices are justified. In this paper, I argue that a major reason for these entrenched disagreements is that problematic metaethical commitments are hidden in these cross-cultural discourses. Using the issue of informed consent in East Asia as an example of one such discourse, I analyze two representative positions in the discussion and identify their metaethical commitments. I suggest that the metaethical assumptions of these positions result from their shared method of ethical justification: moral principlism. I then show why moral principlism is problematic in cross-cultural analyses and propose a more useful method for pursuing ethical justification across cultures.

  2. Algerian National Culture and TQM

    Directory of Open Access Journals (Sweden)

    Abdelkrim Yahia-Berrouiguet

    2015-01-01

    Full Text Available The literature on total quality management confirmed that the national culture has an effect on total quality management implementation. The aim of this study is to compare the Algerian national culture and the TQM culture. The results indicated that not all the national culture dimensions are appropriate for TQM implementation in the Algerian context: the collectivism dimension is appropriate, the power distance and uncertainty avoidance dimensions are not appropriate.

  3. A case study of organisational cultural competence in mental healthcare

    Directory of Open Access Journals (Sweden)

    Bhui Kamaldeep

    2011-09-01

    Full Text Available Abstract Background Ensuring Cultural Competence (CC in health care is a mechanism to deliver culturally appropriate care and optimise recovery. In policies that promote cultural competence, the training of mental health practitioners is a key component of a culturally competent organisation. This study examines staff perceptions of CC and the integration of CC principles in a mental healthcare organisation. The purpose is to show interactions between organisational and individual processes that help or hinder recovery orientated services. Methods We carried out a case study of a large mental health provider using a cultural competence needs analysis. We used structured and semi-structured questionnaires to explore the perceptions of healthcare professionals located in one of the most ethnically and culturally diverse areas of England, its capital city London. Results There was some evidence that clinical staff were engaged in culturally competent activities. We found a growing awareness of cultural competence amongst staff in general, and many had attended training. However, strategic plans and procedures that promote cultural competence tended to not be well communicated to all frontline staff; whilst there was little understanding at corporate level of culturally competent clinical practices. The provider organisation had commenced a targeted recruitment campaign to recruit staff from under-represented ethnic groups and it developed collaborative working patterns with service users. Conclusion There is evidence to show tentative steps towards building cultural competence in the organisation. However, further work is needed to embed cultural competence principles and practices at all levels of the organisation, for example, by introducing monitoring systems that enable organisations to benchmark their performance as a culturally capable organisation.

  4. Culture of cryopreserved rat hepatocyte

    Institute of Scientific and Technical Information of China (English)

    Haitao Yin; Gaojun Teng; Lifeng Wang; Baorui Liu; Xiaoping Qian

    2006-01-01

    Objective: To study the method of cryopreserving rat hepatocytes and double collagen gel culture measurement after its cryopreservation. Methods: Rat hepatocytes, isolated by two-step perfusion with collagenase using an extra corporeal perfusion apparatus, were cryopreserved in double collagen gel with culture medium added by epidermal growth factor(EGF).The expression of cell function and cellular morphology were examined during culture. Results: The hepatocytes cryopreserved in double collagen gel concluding EGF showed good morphology and biological characteristics. After thawing, the MTT metabolism and protein synthesis of hepatocytes in sandwich ± EGF groups were better than those in control group. And the morphology and function of hepatocytes in sandwich group was better than that in EGF group(P < 0.05). Conclusion: Double collagen gel culture can keep hepatocyte's activities. Thawed hepatocytes can be cultivated with collagenous matrix, which provides an environment that more closely resembles that in vivo and maintain the expression of certain liver-specific function of hepatocytes.

  5. Modeling the genealogy of a cultural trait.

    Science.gov (United States)

    Aguilar, Elliot; Ghirlanda, Stefano

    2015-05-01

    The mathematical study of genealogies has yielded important insights in population biology, such as the ability to estimate the time to the most recent common ancestor (MRCA) of a sample of genetic sequences or of a group of individuals. Here we introduce a model of cultural genealogies that is a step toward answering similar questions for cultural traits. In our model individuals can inherit from a variable, potentially large number of ancestors, rather than from a fixed, small number of ancestors (one or two) as is typical of genetic evolution. We first show that, given a sample of individuals, a cultural common ancestor does not necessarily exist. We then introduce a related concept: the most recent unique ancestor (MRUA), i.e., the most recent single individual who is the earliest cultural ancestor of the sample. We show that, under neutral evolution, the time to the MRUA can be staggeringly larger than the time to MRCA in a single ancestor model, except when the average number of learning opportunities per individuals is small. Our results point out that the properties of cultural genealogies may be very different from those of genetic genealogies, with potential implications for reconstructing the histories of cultural traits.

  6. Tetrahydrobiopterin shows chaperone activity for tyrosine hydroxylase.

    Science.gov (United States)

    Thöny, Beat; Calvo, Ana C; Scherer, Tanja; Svebak, Randi M; Haavik, Jan; Blau, Nenad; Martinez, Aurora

    2008-07-01

    Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of catecholamine neurotransmitters. Primary inherited defects in TH have been associated with l-DOPA responsive and non-responsive dystonia and infantile parkinsonism. In this study, we show that both the cofactor (6R)-l-erythro-5,6,7,8-tetrahydrobiopterin (BH(4)) and the feedback inhibitor and catecholamine product dopamine increase the kinetic stability of human TH isoform 1 in vitro. Activity measurements and synthesis of the enzyme by in vitro transcription-translation revealed a complex regulation by the cofactor including both enzyme inactivation and conformational stabilization. Oral BH(4) supplementation to mice increased TH activity and protein levels in brain extracts, while the Th-mRNA level was not affected. All together our results indicate that the molecular mechanisms for the stabilization are a primary folding-aid effect of BH(4) and a secondary effect by increased synthesis and binding of catecholamine ligands. Our results also establish that orally administered BH(4) crosses the blood-brain barrier and therapeutic regimes based on BH(4) supplementation should thus consider the effect on TH. Furthermore, BH(4) supplementation arises as a putative therapeutic agent in the treatment of brain disorders associated with TH misfolding, such as for the human TH isoform 1 mutation L205P.

  7. Ribavirin shows immunomodulatory effects on activated microglia.

    Science.gov (United States)

    Savic, Danijela; Stojiljkovic, Mirjana; Lavrnja, Irena; Parabucki, Ana; Bjelobaba, Ivana; Nedeljkovic, Nadezda; Herdegen, Thomas; Pekovic, Sanja

    2014-12-01

    Abstract Ribavirin (RBV) is synthetic purine nucleoside analogue, licensed as anti-viral drug that displays immunomodulatory actions on various immune cells. Our previous ex vivo studies have demonstrated immunosuppressive effects of RBV on reactive T-lymphocytes in experimental autoimmune encephalomyelitis. Here, we examined the effects of RBV on inflammatory response of microglia. RBV potency to down-regulate microglia inflammatory response was assessed by measuring microglia cell body size, and the production of nitric oxide (NO) and pro- and anti-inflammatory cytokines. RBV exerted cytotoxic effects on LPS-stimulated microglia, leaving non-stimulated microglia unaffected. The exposure of activated microglia to RBV led to: decrease in the level of NO as a result of decreased cell number, lower average cell surface, the reduction of membrane ruffling, the suppression of interleukin-6 release and promoted interleukin-10 production. On the other hand, RBV promoted LPS-induced interleukin-1 beta release. Our results imply that RBV is a complex immunomodulator showing both anti- and pro-inflammatory effects on activated microglia.

  8. Cultural Effects of Trade Liberalization

    OpenAIRE

    Steven M. Suranovic; Robert Winthrop

    2005-01-01

    We incorporate culture into a standard trade model in two distinct ways. In the ¡°cultural affinity from work¡± model, workers receive a non- pecuniary cultural benefit from work in a particular industry. In the ¡°cultural externality¡± model, consumers of a product receive utility from other consumer¡¯s consumption of a domestic good. We show that resistance to change due to cultural concerns can reduce the national benefits from trade liberalization. Complete movements to free trade will ha...

  9. Brand image, cultural events and loyalty in mobile industry

    Directory of Open Access Journals (Sweden)

    Thelma Valéria Rocha

    2013-05-01

    Full Text Available : This study analyses the effect of cultural events in brand image and customers’ loyalty in the mobile industry. The main relevance is to discuss the effectiveness’ of cultural events, those demanding high investments’ for the companies which have to explain the results in terms of loyalty and brand image. The subject is reviewed theoretical considering three topics: cultural events, brand image and loyalty, which lead to hypotheses to be tested. The field research of a quantitative nature uses the survey method, which was applied to 150 teenagers consumers of mobiles companies, classes A and B. The results of field research show that there is a positive relationship between cultural activities and brand image, and between the brand image and loyalty. However, there was significant correlation between cultural events and loyalty only to B Company. It may be related with B events wish were prepared to this target and may improve loyalty. To companies as A and C, brand image acts as moderator between cultural events and loyalty. These results indicated that cultural events may have impact in loyalty, since the company developed those actions to the target. As contribution, this study fills a gap which is the study that discusses effect of cultural activities on brand image and loyalty, in emerging economies, as Brazil, where few researches consider cultural events.

  10. Urine culture - catheterized specimen

    Science.gov (United States)

    Culture - urine - catheterized specimen; Urine culture - catheterization; Catheterized urine specimen culture ... urinary tract infections may be found in the culture. This is called a contaminant. You may not ...

  11. Evaluation of multiple mechanism-based toxicity endpoints in primary cultured human hepatocytes for the identification of drugs with clinical hepatotoxicity: Results from 152 marketed drugs with known liver injury profiles.

    Science.gov (United States)

    Zhang, Jie; Doshi, Utkarsh; Suzuki, Ayako; Chang, Ching-Wei; Borlak, Jürgen; Li, Albert P; Tong, Weida

    2016-08-05

    We report here the results of a collaborative research program to develop a robust and reliable in vitro system to allow an accurate definition of the drug-induced liver injury (DILI) potential of new drug entities during drug development. The in vitro hepatotoxic potential of 152 drugs with known DILI profiles were evaluated in primary cultured human hepatocytes with four mechanistically-relevant endpoints: cellular ATP depletion, reactive oxygen species (ROS), glutathione (GSH) depletion, and caspase activation for apoptosis. The drugs, 80 in the testing set and 72 in the validation set, were classified based on serious clinical/regulatory outcomes as defined by reported acute liver failure, black-box warning, and/or withdrawal. The drugs were further sub-categorized for dominant types of liver injury. Logistic regression models were performed to calculate the area under the receiver operating characteristics curve (AUROC) and to evaluate the prediction potential of the selected endpoints for serious clinical/regulatory outcomes. The ROS/ATP ratio was found to yield an excellent AUROC in both the testing (0.8989, P drugs associated with severe from non-severe DILI cases (p drugs in primary human hepatocytes using the ROS/ATP ratio endpoint may aid the definition of their potential to cause severe DILI.

  12. Class, Culture and Politics

    DEFF Research Database (Denmark)

    Harrits, Gitte Sommer

    2013-01-01

    Even though contemporary discussions of class have moved forward towards recognizing a multidimensional concept of class, empirical analyses tend to focus on cultural practices in a rather narrow sense, that is, as practices of cultural consumption or practices of education. As a result......, discussions within political sociology have not yet utilized the merits of a multidimensional conception of class. In light of this, the article suggests a comprehensive Bourdieusian framework for class analysis, integrating culture as both a structural phenomenon co-constitutive of class and as symbolic...

  13. Does cultural capital really affect academic achievement?

    DEFF Research Database (Denmark)

    Jæger, Mads Meier

    2011-01-01

    This paper provides new estimates of the causal effect of cultural capital on academic achievement. The author analyzes data from the National Longitudinal Survey of Youth – Children and Young Adults and uses a fixed effect design to address the problem of omitted variable bias which has resulted...... cultural mobility theory (cultural capital more important in low-SES environments) for indicators capturing “concerted cultivation.”...... previously reported; and (3) the effect of cultural capital varies in high- and low-SES environments. Results mostly support cultural reproduction theory (cultural capital more important in high-SES environments) for cultural capital indicators capturing familiarity with legitimate culture and mostly support...

  14. A Cultural Sexuality or a Sexual Culture?

    NARCIS (Netherlands)

    Vandermeersch, Patrick

    1990-01-01

    P. Vandermeersch, A Cultural Sexuality or a Sexual Culture? In: F. VAN DE VIJVER & G. HUTSCHEMAEKERS (ed.), The Investigation of Culture. Current Issues in Cultural Psychology, Tilburg, Tilburg University Press, 1990, 43-58.

  15. LINGUISTIC ANALYSIS OF ECONOMIC TERMS IN CULTURAL CONTEXTS

    Directory of Open Access Journals (Sweden)

    Gurenkova, Y.V.

    2017-06-01

    Full Text Available This article is the result of a comparative analysis of five economic terms ("business", "competition", "salary", "boss", "profit" in the context of the culture of Russia and the United States. The research shows that some words were borrowed in both languages from different sources (for example, "salary", some of them were borrowed from one source (for example, "business", and some were received from own original words (for example, "profit", "boss". Comparison of cultural contexts and dictionary entries shows that the terms analyzed in this paper primarily help to understand how the two countries (Russia and the US can be culturally different. The study also illustrates how these terms have developed their own additional meanings, characteristic of their cultural and social development. Consequently, certain concepts can be transformed through the prism of a cultural vision of the world of different peoples.

  16. Retinoblastoma pathway defects show differential ability to activate the constitutive DNA damage response in human tumorigenesis

    DEFF Research Database (Denmark)

    Tort, F.; Bartkova, J.; Sehested, M.

    2006-01-01

    Loss of G(1)-S control and aberrations of the p16(Ink4a)-cyclin D1/cyclin-dependent kinase (CDK) 4(6)-pRb-E2F-cyclin E/CDK2 pathway are common in human cancer. Previous studies showed that oncogene-induced aberrant proliferation, such as on cyclin E overexpression, causes DNA damage and checkpoint...... culture models with differential defects of retinoblastoma pathway components, as overexpression of cyclin D1 or lack of p16(Ink4a), either alone or combined, did not elicit detectable DDR. In contrast, inactivation of pRb, the key component of the pathway, activated the DDR in cultured human or mouse...... cells, analogous to elevated cyclin E. These results highlight differential effect of diverse oncogenic events on driving the 'cancer cell cycles' and their ability to deregulate the replication-driving CDK2 kinase and to alarm the DDR as a potential anticancer barrier in accordance...

  17. 降钙素原和超敏C反应蛋白预测败血症血液培养阳性的临床价值%Clinical value of procalcitonin and hs-CRP in predicting positive blood culture results in sepsis

    Institute of Scientific and Technical Information of China (English)

    于波海; 余敏红; 张萌; 柴淼; 苏丽菊; 高春波; 孙琦; 滕旭

    2015-01-01

    Objective To investigate and compare the clinical values of serum procalcitonin (PCT)and high sensitivity C-reac-tive protein (hs-CRP)levels for predicting the blood culture positivity in the patients with sepsis.Methods 132 adult patients with sepsis were enrolled in this study.Blood cultures were performed before the antibacterial therapy.The white blood cell (WBC) count,absolute neutrophil count(ANC),levels of PCT and hs-CRP were determined.The application value of PCT and hs-CRP for predicting the positive blood culture results were evaluated.Results The median serum PCT levels in the blood culture positive group and the blood culture negative group were 7.92 ng/mL and 0.95 ng/mL respectively,the difference had statistical signifi-cance(P <0.01).The receiver operating characteristic (ROC)curves showed that PCT had a higher predictive accuracy for blood culture positivity compared with hs-CRP,the area under the curve (AUC)was 0.810(P =0.001)and 0.690(P =0.274),respec-tively.The combined detection of PCT and hs-CRP for predicting the blood culture positive results was similar to the performance of PCT alone,AUC as 0.885 (P =0.001 ).The median cut point of PCT was 0.91 ng/mL,the sensitivity of PCT for predicting blood culture positivity was 90%.This sensitivity remained unchanged when PCT cut point was1.14ng/mL.Using the PCT cut points of 0.91 and 1.14 enabled reducing the submitted blood cultures by 51% and 56% respectively.Conclusion Compared with hs-CRP,serum PCT level could better predict the blood culture positivity in the patients with sepsis.%目的:探讨和比较降钙素原(PCT)和超敏 C 反应蛋白(hs-CRP)预测败血症患者血液培养阳性的临床价值。方法132例败血症患者纳入该研究,在应用抗菌药物治疗前进行血液培养实验,并检测白细胞计数、中性粒细胞绝对值、PCT、hs-CRP,评价 PCT 和 hs-CRP 预测血液培养阳性的应用价值。结果血液培养

  18. Workshop on cultural usability and human work interaction design

    DEFF Research Database (Denmark)

    Clemmensen, Torkil; Ørngreen, Rikke; Roese, Kerstin

    2008-01-01

    , Workplace observation, Think-Aloud Usability Test, etc. These techniques often give - seemingly - similar results when applied in diverse cultural settings, but experience shows that we need a deep understanding of the cultural, social and organizational context to interpret the results, and to transform...... and climate, etc.). See conference website http://www.nordichi2008.org/index.php?option=com_content&task=view&id=68&Itemid=92 and workgroup website at http://ilex.cbs.dk/hwid/, where the proceedings is available....

  19. Splitting culture medium by air-jet and rewetting for the assessment of the wettability of cultured epithelial cell surfaces.

    Science.gov (United States)

    Tanaka, Nobuyuki; Kondo, Makoto; Uchida, Ryohei; Kaneko, Makoto; Sugiyama, Hiroaki; Yamato, Masayuki; Okano, Teruo

    2013-12-01

    This study found that the phenomenon of rewetting after squeezing culture medium varied in different culture conditions for rat oral mucosal epithelial cells. When culture medium covering over cultured cells was squeezed by an air-jet application, the motion of squeezed culture medium was able to be observed by using a commercially available movie camera. Squeezed width on cells cultured in keratinocyte culture medium (KCM), which contained with fetal bovine serum, was one-sixth of that in FBS-free KCM. This result corresponded to the mucous layer staining statuses of cultured cells in both cases; positive in KCM and negative in FBS-free medium. Furthermore, the gene expression of mucous glycoprotein MUC4 in KCM was 100 times higher than that in FBS-free medium, and the expression of MUC4 protein only showed on the apical surface of cells cultured in KCM. The relative gene expression levels of MUC1, 13, 15, and 16 in both the normal and FBS-free medium were found to be no more than one-thirtieth of that of MUC4 in KCM. The main factor of the wettability difference between KCM and FBS-free medium was speculated to be the difference of MUC4 expression between both media. This method can be a simple technique for testing not only the surface wettability but also the mucous formation of cultured cells. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. Cultural Robotics: The Culture of Robotics and Robotics in Culture

    OpenAIRE

    2013-01-01

    In this paper, we have investigated the concept of "Cultural Robotics" with regard to the evolution of social into cultural robots in the 21st Century. By defining the concept of culture, the potential development of a culture between humans and robots is explored. Based on the cultural values of the robotics developers, and the learning ability of current robots, cultural attributes in this regard are in the process of being formed, which would define the new concept of cultural robotics. Ac...

  1. CREATING VALUE WITHIN CONSUMPTION CULTURE

    Directory of Open Access Journals (Sweden)

    Daniel Adrian Gârdan

    2017-01-01

    Full Text Available The issue of culture consumption is a particular concern within the modern marketing theory. Culture can be seen as representing a body of knowledge, beliefs, values, attitudes, symbols etc, developed in a certain period of time by a group of individuals, items transmitted with the help of a social learning process to other generations within the group. Thus, the consumption of culture will identify itself with the consumption of any product, service or a combination of them, directly resulted as manifestation of culture, expressions of artistic creativity specific for a certain cultural space. The present paper proposes the analysis of the phenomenon referring to the culture consumption in terms of specific characteristics. The paper reviews the features specific to the modern consumer of culture, the relationship that exists between the individuals’ level of education and the culture consumption and value creation process or augmentation of the intrinsic value of an artistic product as a result of the contribution that the culture consumer can bring himself. The authors highlight the fact that within extremely complex processes which are defining the culture consumption, consumers can assume an active role, becoming on their turn co-participants in the cultural goods and services value creation and transmission. The modern consumer benefits more than ever from the advantages offered by the information technology, being called to respond to major challenges of the postmodernism paradigm in terms of culture consumption. Globalization and other social economic and politic phenomena have profoundly changed the reports between individual and culture, between self and other members of the society, causing synthesis and essential transformations of culture consumption, of culture consumers typologies, and not least of the very forms of artistic expression related to cultural goods and services.

  2. NASA GIBS Use in Live Planetarium Shows

    Science.gov (United States)

    Emmart, C. B.

    2015-12-01

    The American Museum of Natural History's Hayden Planetarium was rebuilt in year 2000 as an immersive theater for scientific data visualization to show the universe in context to our planet. Specific astrophysical movie productions provide the main daily programming, but interactive control software, developed at AMNH allows immersive presentation within a data aggregation of astronomical catalogs called the Digital Universe 3D Atlas. Since 2006, WMS globe browsing capabilities have been built into a software development collaboration with Sweden's Linkoping University (LiU). The resulting Uniview software, now a product of the company SCISS, is operated by about fifty planetariums around that world with ability to network amongst the sites for global presentations. Public presentation of NASA GIBS has allowed authoritative narratives to be presented within the range of data available in context to other sources such as Science on a Sphere, NASA Earth Observatory and Google Earth KML resources. Specifically, the NOAA supported World Views Network conducted a series of presentations across the US that focused on local ecological issues that could then be expanded in the course of presentation to national and global scales of examination. NASA support of for GIBS resources in an easy access multi scale streaming format like WMS has tremendously enabled particularly facile presentations of global monitoring like never before. Global networking of theaters for distributed presentations broadens out the potential for impact of this medium. Archiving and refinement of these presentations has already begun to inform new types of documentary productions that examine pertinent, global interdependency topics.

  3. Cultural Resurrection

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    "Who are we?Where are we from?"Humans have been pondering these questions since the day they first came into being.One of the ways we preserve memories of the past is through our cul- tural heritage that has been passed on from generation to genera- tion.Intangible cultural heritage,as well as tangible cultural her- itage,is essential to the continuity of human civilization. Since the United Nations Educational,Scientific and Cultural Organization(UNESCO)unveiled the Masterpieces of the Oral and Intangible Heritage of Humanity in 2001,China has had Kunqu opera,Guqin and its music,the art of Uygur Muqam of Xinjiang and the traditional Mongolian folk song Long Song added to UNESCO’s protection list.It is now one of the coun-

  4. Japanese Shame Culture and American Guilt Culture

    Institute of Scientific and Technical Information of China (English)

    Lu Weijie

    2016-01-01

    Culture is an important factor contributing to the success of intercultural communication. In the east and west, there are many different cultures, among which Japanese shame culture and American guilt culture are two typical ones. Influenced by different cultures, these two countries have different characteristics, which reminds us that in intercultural communication culture should be paid much attention to.

  5. Culturally Responsive Teaching: Understanding Disability Culture

    Science.gov (United States)

    Darrow, Alice-Ann

    2013-01-01

    To be culturally responsive teachers, we must first have an understanding of other cultures and how students from these cultures differ from one another. As we consider the many cultures represented in our classrooms, we might also consider students with disabilities as a cultural group. Within any main culture are subgroups differentiated by…

  6. MRI shows clodronate-liposomes attenuating liverinjuryinratswithsevereacutepancreatitis

    Institute of Scientific and Technical Information of China (English)

    Jian-Xin Zhang; Sheng-Chun Dang; Yong Zhang; Xin Sha; Li-Rong Zhang; Chuan-She Wei; Min Chen; De-Li Jiang

    2010-01-01

    BACKGROUND: Studies have revealed that macrophages play an important role in the development of severe acute pancreatitis (SAP). Activated macrophages can lead to a systemic inlfammatory response, induce lipid peroxidation, impair membrane structure, result in injury to the liver and the other extrahepatic organs, and eventually result in multiple organ dysfunction syndrome by promoting excessive secretion of cytokines. Liver injury can further aggravate the systemic inlfammatory response and increase mortality by affecting the metabolism of toxins and the release of excessive inlfammatory mediators. Clodronate is a synthetic bisphosphonate, which is often used for treating bone changes caused by osteoporosis and other factors. In the current study, we created liposomes containing superparamagnetic iron oxide particles (SPIOs) for macrophage labeling and magnetic resonance imaging, using a novel method that can bind the clodronate to induce apoptosis and deplete macrophages. METHODS: Superparamagnetic Fe3O4 nanoparticles were prepared by chemical coprecipitation. SPIO-containing liposomes and SPIO-clodronate-containing liposomes were prepared by the thin iflm method. SAP models were prepared by injection of sodium taurocholate (2 ml/kg body weight) into the subcapsular space of the pancreas. Sprague-Dawley rats were randomly divided into a control group, a SAP plus SPIO-liposome group, and a SAP plus SPIO-clodronate-containing group. Two and six hours after SAP models were available, T2-weighted MRI scans (in the same plane) of the livers of rats in each group were performed. At the end of the scans, 2 ml of blood was taken from the superior mesenteric vein to measure the levels of serum amylase, ALT, AST, TNF-α, and IL-6. Pathological changes in the liver and pancreas were assessed. RESULTS: Transmission electron microscopy showed that the liposomes had a uniform size. No pathological changes in the pancreata of rats in the control group were noted. The

  7. Natural Product Shows Effectiveness in Combating Colorectal Cancer | Poster

    Science.gov (United States)

    An herbal extract used for centuries to prevent heart disease has now been shown to be effective against colorectal cancer when tested in laboratory cell cultures. Scientists from NCI at Frederick found that the natural extract cryptotanshinone (CPT) stops the uncontrolled cell growth characteristic of cancer by interfering with a protein that has been implicated in several cancers, including those of the colon and rectum. The results appear in the journal Molecular and Cellular Biochemistry.

  8. Safety Culture in Indian Hospitals: A Cultural Adaptation of the Safety Attitudes Questionnaire.

    Science.gov (United States)

    Patel, Saharsh; Wu, Albert W

    2016-06-01

    Patient safety is increasingly recognized as a global health concern because of a staggering number of health care-related injuries and deaths. Although many hospitals are attempting to promote a patient safety agenda, there are relatively few options to track progress. The aims of this study were to translate and culturally adapt the Safety Attitudes Questionnaire (SAQ) into Gujarati and to provide evidence for its reliability and validity in hospitals in Gujarat, India. We also present preliminary data on the safety culture in these organizations. The first phase was the cultural adaptation and translation of the SAQ into Gujarati. The second phase was a cross-sectional survey of safety attitudes in 4 private hospitals. The survey was distributed to 424 health care workers and elicited an overall response rate of 79%. The questionnaire showed acceptable reliability and preliminary evidence for construct validity among health care workers in 4 private hospitals of varying size. The initial culture score results showed outcomes similar to international standards, with two-thirds of the respondents describing teamwork climate positively and more than half of the respondents describing safety climate positively. This study reveals promising initial results for patient safety culture in India, but further study is needed. The development and validation of the SAQ-Gujarati allow additional hospitals to evaluate their patient safety culture. As the first rendition of the SAQ to an Indian setting, the tool could help to initiate safety discourse and improve the potential for institutions to provide feedback to their staff members.

  9. Cultural Transmission on the Taskscape: Exploring the Effects of Taskscape Visibility on Cultural Diversity

    Science.gov (United States)

    Premo, L. S.; Tostevin, Gilbert B.

    2016-01-01

    Culturally transmitted behavior can be structured in its performance both geographically and temporally, in terms of where and when implements are made and used on the landscape (what Ingold calls “the taskscape”). Yet cultural transmission theory has not yet explored the consequences of behaviors transmitted differently due to their enactment at different taskscape locations, what Tostevin calls “taskscape visibility.” Here, we use computer simulations to explore how taskscape visibility and forager mobility affect the diversity of two selectively neutral culturally transmitted traits within a single population of social learners. The trait that can be transmitted from residential bases only (lower taskscape visibility) shows greater diversity than the trait that can be transmitted from residential bases and logistical camps (higher taskscape visibility). In addition, increased logistical mobility has a positive effect on the diversity of the trait with the lower taskscape visibility while it generally shows little to no effect on the diversity of the trait with higher taskscape visibility. Without an appreciation for the ways in which taskscape visibility and mobility can structure cultural transmission in space and through time, the difference in the observed equilibrium diversity levels of the two traits might be incorrectly interpreted as resulting from qualitatively different forms of biased cultural transmission. The results of our simulation experiment suggest that researchers may need to take the taskscape visibility into account when inferring cultural transmission from archaeological data. PMID:27583682

  10. Cultural Transmission on the Taskscape: Exploring the Effects of Taskscape Visibility on Cultural Diversity.

    Science.gov (United States)

    Premo, L S; Tostevin, Gilbert B

    2016-01-01

    Culturally transmitted behavior can be structured in its performance both geographically and temporally, in terms of where and when implements are made and used on the landscape (what Ingold calls "the taskscape"). Yet cultural transmission theory has not yet explored the consequences of behaviors transmitted differently due to their enactment at different taskscape locations, what Tostevin calls "taskscape visibility." Here, we use computer simulations to explore how taskscape visibility and forager mobility affect the diversity of two selectively neutral culturally transmitted traits within a single population of social learners. The trait that can be transmitted from residential bases only (lower taskscape visibility) shows greater diversity than the trait that can be transmitted from residential bases and logistical camps (higher taskscape visibility). In addition, increased logistical mobility has a positive effect on the diversity of the trait with the lower taskscape visibility while it generally shows little to no effect on the diversity of the trait with higher taskscape visibility. Without an appreciation for the ways in which taskscape visibility and mobility can structure cultural transmission in space and through time, the difference in the observed equilibrium diversity levels of the two traits might be incorrectly interpreted as resulting from qualitatively different forms of biased cultural transmission. The results of our simulation experiment suggest that researchers may need to take the taskscape visibility into account when inferring cultural transmission from archaeological data.

  11. Information cultures

    DEFF Research Database (Denmark)

    2017-01-01

    The purpose of this article is to suggest a genealogy of the concept of information beyond the 20th century. The article discusses how the concept of information culture might provide a way of formulating such a genealogic strategy. The article approaches this purpose by providing a general...... narrative of premodern information cultures, examining works on early-modern scholars and 18th century savants and discussion of what seems to be a Foucauldian rupture in the conceptualization of information in 19th century England. The findings of the article are situated in the thinking that a genealogy...... of information would reveal that information had specific purposes in specific settings....

  12. Culture Consciousness

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    This year, June 10 marked China's first Cultural Heritage Day. The designation by the Chinese Government aims to raise awareness of the need to protect and understand the value of the nation's abundant cultural treasures. In future the second Saturday in June each year will be set aside for this purpose. Recently, the State Council published the sixth group of major relics under state protection. On the list are 1,080 historic relics such as the Grand Canal from Beijing to Hangzhou and the

  13. Boosting Culture

    Institute of Scientific and Technical Information of China (English)

    ZHOU JIANXIONG

    2011-01-01

    Culture makes up an indispensable part of our lives,just like material comfort.It is thought of as an important source of a nation's vitality and creativity,and constitutes a key factor uniting the nation,while making it distinctive from other countries.It is also said culture is a productive power that not only shapes human concepts and impacts their behavior,but also contributes in no small measure to the betterment of our material as well as spiritual world.

  14. MARKETING CULTURAL

    OpenAIRE

    Ramírez, Claudia Gómez

    2013-01-01

    Este artículo analiza la definición de "Marketing" Cultural y la adaptación y beneficios del "marketing" tradicional respecto al conjunto de manifestaciones artísticas de las diversas industrias involucradas en el sector cultural o artístico; asimismo, se desagregan los conceptos básicos que lo componen como factor de éxito en dichas empresas. Se hace uso de la exposición de casos específicos para ilustrar la articulación de estos dos conceptos aparentemente contrapuestos, cultura y "marketin...

  15. Do men and women show love differently in marriage?

    Science.gov (United States)

    Schoenfeld, Elizabeth A; Bredow, Carrie A; Huston, Ted L

    2012-11-01

    In Western societies, women are considered more adept than men at expressing love in romantic relationships. Although scholars have argued that this view of love gives short shrift to men's ways of showing love (e.g., Cancian, 1986; Noller, 1996), the widely embraced premise that men and women "love differently" has rarely been examined empirically. Using data collected at four time points over 13 years of marriage, the authors examined whether love is associated with different behaviors for husbands and wives. Multilevel analyses revealed that, counter to theoretical expectations, both genders were equally likely to show love through affection. But whereas wives expressed love by enacting fewer negative or antagonistic behaviors, husbands showed love by initiating sex, sharing leisure activities, and doing household work together with their wives. Overall, the findings indicate that men and women show their love in more nuanced ways than cultural stereotypes suggest.

  16. Cultural psychiatry: a general perspective.

    Science.gov (United States)

    Alarcón, Renato D

    2013-01-01

    The current scene in the field of cultural psychiatry shows a vigorous growth, multifaceted conceptual and research developments and more relevant clinical presence. After a pertinent definition of the discipline, this chapter examines the contribution of cultural psychiatry to the etiopathogenesis of mental disorders, to the variations of clinical presentations in numerous entities, to psychiatric diagnosis and treatment and to the relatively unexplored rubric of preventive psychiatry. Advanced concepts of neurosciences and technology-based research can find a place in the realm of biocultural correlates. The role of culture in the definition of mental illness, the renewed notions of the old 'culture-bound syndromes', hope, cognition and culture in psychiatric treatments (including the so-called 'cultural therapies'), and resiliency are areas duly examined and discussed. Cultural psychiatry has re-emerged as a reliable body of knowledge aimed at a comprehensive assessment of human beings as patients.

  17. Study on in vitro Cultural Behaviors of Spermatogonium Stem Cells of New Born Calves

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jinyou; HU Pengfei; HUANG Zhijun; LV Zhonghua; ZHANG Guixue

    2008-01-01

    Three methods were adopted in culture spermatogoniums of newly born calf in vitro, such as enzymatic digestion and percoll density gradient centrifugation(Method I ), tubular fragments culture(Method Ⅱ ) and tissue culture(Method Ⅲ), and cultural behaviors of cells were observed. The results showed that typical spermatogonium colonies appeard at 144 h of culture by enzymatic digestion-percoll density gradient centrifugation method and tubular fragments culture method, 2.5% FBS kept the characteristics of spermatogonium stem cell better than others, produced more mass clones, and FBS of more than 2.5% concentration benefited spermatogonium differentiation and the number of colonies was significantly affected by FBS concentration. After 1 week of culture in method Ⅲ, the diameter of lumens and quantity of sertoli's cells in tubal wall increased obviously, lumen of seminiferous tubules appeared. Sertoli's cells kept constant and the number of spermatogoniums decreased obviously after 2 weeks of culture.

  18. Molluscan cells in culture: primary cell cultures and cell lines

    OpenAIRE

    2013-01-01

    In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as bi...

  19. Cyclic tensile stretch load and oxidized low density lipoprotein synergistically induce lectin-like oxidized ldl receptor-1 in cultured bovine chondrocytes, resulting in decreased cell viability and proteoglycan synthesis.

    Science.gov (United States)

    Akagi, Masao; Nishimura, Shunji; Yoshida, Kohji; Kakinuma, Takumi; Sawamura, Tatsuya; Munakata, Hiroshi; Hamanishi, Chiaki

    2006-08-01

    Mechanical stimulation is known to be an essential factor in the regulation of cartilage metabolism. We tested the hypothesis that expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) can be modulated by cyclic tensile stretch load in chondrocytes. Cyclic loading of repeated stretch stress at 10 cycles per minute with 10 kPa of stress for 6 h induced expression of LOX-1 to 2.6 times control in cultured bovine articular chondrocytes, equivalent to the addition of 10 microg/mL oxidized low density lipoprotein (ox-LDL) (2.4 times control). Application of the cyclic load to the chondrocytes along with 10 microg/mL ox-LDL resulted in synergistically increased LOX-1 expression to 6.3 times control. Individual application of cyclic loading and 10 microg/mL ox-LDL significantly suppressed chondrocytes viability (84.6% +/- 3.4% and 80.9% +/- 3.2% of control at 24 h, respectively; n = 3; p LDL (n = 3)]. Cyclic loading and 10 microg/mL ox-LDL synergistically affected cell viability and proteoglycan synthesis, which were significantly suppressed to 45.6% +/- 4.9% and 48.7% +/- 6.7% of control at 24 h, respectively (n = 3; p LDL). In this study, we demonstrated synergistic effects of cyclic tensile stretch load and ox-LDL on cell viability and proteoglycan synthesis in chondrocytes, which may be mediated through enhanced expression of LOX-1 and which has important implications in the progression of cartilage degeneration in osteoarthritis.

  20. Geology's Impact on Culture

    Science.gov (United States)

    Pizzorusso, Ann

    2017-04-01

    Most people consider geology boring, static and difficult. The fields of astronomy and physics have "rebranded" themselves with exciting programs formatted so as to be readily understandable to the general public. The same thing can be done for geology. My research on geology's influence on other disciplines has resulted in a book, Tweeting da Vinci, in which I was able to show how geology affected Italy's art, architecture, medicine, religion, literature, engineering and just about everything else. The reaction to the book and my lectures by both students and the general public has been very positive, including four gold medals, with reviews and comments indicating that they never knew geology could be so exciting. The book is very user friendly, packed with facts, full-color photos, paintings, sketches and illustrations. Complex aspects of geology are presented in an easily understandable style. Widely diverse topics—such as gemology, folk remedies, grottoes, painting, literature, physics and religion—are stitched together using geology as a thread. Quoting everyone from Pliny the Elder to NASA physicist Friedemann Freund, the work is solidly backed scholarship that reads as easily as a summer novel. The book can be used in classes such as physics, chemistry, literature, art history, medicine, Classical Studies, Latin, Greek and Italian. By incorporating a "geologic perspective" in these courses, it can be perceived as a more "all encompassing" discipline and encourage more students to study it. The lectures I have given on college campuses have resulted in students seeing their own majors from a different perspective and some have even signed up for introductory geology courses. One college organized summer course to the Bay of Naples based on the book. We followed the geology as well as the culture of the area and the students were profoundly moved. To encourage dialog, the book is linked to Facebook, Twitter and Instagram. This has enabled followers from