WorldWideScience

Sample records for culture media components

  1. 21 CFR 864.2220 - Synthetic cell and tissue culture media and components.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Cell And Tissue Culture Products § 864.2220 Synthetic cell and tissue culture media and components. (a) Identification. Synthetic... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Synthetic cell and tissue culture media...

  2. Cell culture medium improvement by rigorous shuffling of components using media blending.

    Science.gov (United States)

    Jordan, Martin; Voisard, Damien; Berthoud, Antoine; Tercier, Laetitia; Kleuser, Beate; Baer, Gianni; Broly, Hervé

    2013-01-01

    A novel high-throughput methodology for the simultaneous optimization of many cell culture media components is presented. The method is based on the media blending approach which has several advantages as it works with ready-to-use media. In particular it allows precise pH and osmolarity adjustments and eliminates the need of concentrated stock solutions, a frequent source of serious solubility issues. In addition, media blending easily generates a large number of new compositions providing a remarkable screening tool. However, media blending designs usually do not provide information on distinct factors or components that are causing the desired improvements. This paper addresses this last point by considering the concentration of individual medium components to fix the experimental design and for the interpretation of the results. The extended blending strategy was used to reshuffle the 20 amino acids in one round of experiments. A small set of 10 media was specifically designed to generate a large number of mixtures. 192 mixtures were then prepared by media blending and tested on a recombinant CHO cell line expressing a monoclonal antibody. A wide range of performances (titers and viable cell density) was achieved from the different mixtures with top titers significantly above our previous results seen with this cell line. In addition, information about major effects of key amino acids on cell densities and titers could be extracted from the experimental results. This demonstrates that the extended blending approach is a powerful experimental tool which allows systematic and simultaneous reshuffling of multiple medium components.

  3. HEK293 cell culture media study towards bioprocess optimization: Animal derived component free and animal derived component containing platforms.

    Science.gov (United States)

    Liste-Calleja, Leticia; Lecina, Martí; Cairó, Jordi Joan

    2014-04-01

    The increasing demand for biopharmaceuticals produced in mammalian cells has lead industries to enhance bioprocess volumetric productivity through different strategies. Among those strategies, cell culture media development is of major interest. In the present work, several commercially available culture media for Human Embryonic Kidney cells (HEK293) were evaluated in terms of maximal specific growth rate and maximal viable cell concentration supported. The main objective was to provide different cell culture platforms which are suitable for a wide range of applications depending on the type and the final use of the product obtained. Performing simple media supplementations with and without animal derived components, an enhancement of cell concentration from 2 × 10(6) cell/mL to 17 × 10(6) cell/mL was achieved in batch mode operation. Additionally, the media were evaluated for adenovirus production as a specific application case of HEK293 cells. None of the supplements interfered significantly with the adenovirus infection although some differences were encountered in viral productivity. To the best of our knowledge, the high cell density achieved in the work presented has never been reported before in HEK293 batch cell cultures and thus, our results are greatly promising to further study cell culture strategies in bioreactor towards bioprocess optimization. Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  4. Screening of biofilm formation by beneficial vaginal lactobacilli and influence of culture media components.

    Science.gov (United States)

    Terraf, M C Leccese; Juárez Tomás, M S; Nader-Macías, M E F; Silva, C

    2012-12-01

    To assess the ability of vaginal lactobacilli to form biofilm under different culture conditions and to determine the relationship between their growth and the capability of biofilm formation by selected strains. Fifteen Lactobacillus strains from human vagina were tested for biofilm formation by crystal violet staining. Only Lactobacillus rhamnosus Centro de Referencia para Lactobacilos Culture Collection (CRL) 1332, Lact. reuteri CRL 1324 and Lact. delbrueckii CRL 1510 were able to grow and form biofilm in culture media without Tween 80. However, Lact. gasseri CRL 1263 (a non-biofilm-forming strain) did not grow in these media. Scanning electron microscopy showed that Lact. rhamnosus CRL 1332 and Lact. reuteri CRL 1324 formed a highly structured biofilm, but only Lact. reuteri CRL 1324 showed a high amount of extracellular material in medium without Tween. Biofilm formation was significantly influenced by the strain, culture medium, inoculum concentration, microbial growth and chemical nature of the support used for the assay. The results allow the selection of biofilm-forming vaginal Lactobacillus strains and the conditions and factors that affect this phenomenon. © 2012 The Society for Applied Microbiology.

  5. Benefits and Limitations of Protein Hydrolysates as Components of Serum-Free Media for Animal Cell Culture Applications

    Science.gov (United States)

    Lobo-Alfonso, Juliet; Price, Paul; Jayme, David

    Increased understanding of influential factors for the cultivation of animal cells, combined with heightened regulatory concern over potential transmission of adventitious contaminants associated with serum and other animal-derived components, has elevated interest in using protein hydrolysates as serum replacements or nutrient supplements. This paper reviews the chemistry and biology of various hydrolysates derived from animal, plant and microbial sources. It provides specific examples of a beneficial selection of plant and yeast hydrolysates as ingredients of serum-free nutrient formulations for bioproduction applications of cultured mammalian and insect cells. Strategies for customizing and optimizing nutrients for specialized applications and general benefits and limitations of protein hydrolysates for biopharmaceutical production are also discussed.

  6. Games culture and media practices

    Directory of Open Access Journals (Sweden)

    Pau Alsina

    2007-05-01

    Full Text Available Our aim in this article is to explore the relationship between videogames and other practices related to audiovisual media in everyday life; we are specifically interested in examining how far videogames, as a cultural form that combines audiovisual narrative with the fun of a game, may be useful in understanding broader cultural transformations in relation to cultural production in the new media context opened up by information and communication technologies.

  7. Social Media as Leisure Culture

    DEFF Research Database (Denmark)

    Albrechtslund, Anne-Mette Bech; Albrechtslund, Anders

    2014-01-01

    The purpose of this paper is to situate the everyday use of social media in the broader cultural practice of leisure. Whereas the use of social media has many different aims and contexts, our main idea is to emphasize how social media practices associated with leisure and playfulness rather than...... functionality and tasks — therefore seemingly “useless” in a strictly utilitarian sense — are practices which are meaningful. We point to certain dynamics in social media practices which we connect to the culture of twentieth century mass tourism, using observations of central touristic practices to motivate...... an analysis of social media use as leisure culture. This gives us a nuanced understanding of the activities connecting everyday life and social media. Further, our analysis provides new insights into the basic motivation for engaging in online sociality despite concerns about privacy, time...

  8. Social Media as Leisure Culture

    DEFF Research Database (Denmark)

    Albrechtslund, Anne-Mette Bech; Albrechtslund, Anders

    2014-01-01

    The purpose of this paper is to situate the everyday use of social media in the broader cultural practice of leisure. Whereas the use of social media has many different aims and contexts, our main idea is to emphasize how social media practices associated with leisure and playfulness rather than...... functionality and tasks — therefore seemingly “useless” in a strictly utilitarian sense — are practices which are meaningful. We point to certain dynamics in social media practices which we connect to the culture of twentieth century mass tourism, using observations of central touristic practices to motivate...... an analysis of social media use as leisure culture. This gives us a nuanced understanding of the activities connecting everyday life and social media. Further, our analysis provides new insights into the basic motivation for engaging in online sociality despite concerns about privacy, time...

  9. Micropolitics of Media Culture

    NARCIS (Netherlands)

    Pisters, Patricia

    2001-01-01

    This book focuses on the micro-political implications of the work of Gilles Deleuze (and Felix Guattari). General philosophical articles are coupled to more specific analyses of films (such as Fight Club and Schindler's List) and other expressions of contemporary culture. The choice of giving specif

  10. Micropolitics of Media Culture

    NARCIS (Netherlands)

    Pisters, Patricia

    2001-01-01

    This book focuses on the micro-political implications of the work of Gilles Deleuze (and Felix Guattari). General philosophical articles are coupled to more specific analyses of films (such as Fight Club and Schindler's List) and other expressions of contemporary culture. The choice of giving specif

  11. Rethinking Popular Culture and Media

    Science.gov (United States)

    Marshall, Elizabeth, Ed.; Sensoy, Ozlem, Ed.

    2011-01-01

    "Rethinking Popular Culture and Media" is a provocative collection of articles that begins with the idea that the "popular" in classrooms and in the everyday lives of teachers and students is fundamentally political. This anthology includes outstanding articles by elementary and secondary public school teachers, scholars, and activists who…

  12. Media Translation and Cultural Soft Power

    Institute of Scientific and Technical Information of China (English)

    段蕾

    2015-01-01

    Media translation is a kind of applied translation,it is the symbol of a country’s development,and the demonstration of its cultural soft power.This essay mainly talks about how does media translation affect a country’s cultural soft power from three forms of media translation—news,movie and television,newspapers and magazines.

  13. Media Translation and Cultural Soft Power

    Institute of Scientific and Technical Information of China (English)

    段蕾

    2015-01-01

    Media translation is a kind of applied translation,itis the symbol of a country’s development,and the demonstration of its cultural soft power.This essay mainly talks about how does media translation affect a country’s cultural soft power from three forms of media translation-news,movie and television,newspapers and magazines.

  14. Personality, media preferences, and cultural participation

    NARCIS (Netherlands)

    Kraaykamp, Gerbert; Eijck, Koen van

    2005-01-01

    In this paper, the impact of the Big Five personality factors (extraversion, friendliness, conscientiousness, emotional stability, and openness) on media preferences (TV programs) and cultural participation (book reading and attending museums and concerts) was examined. The analyses were carried out

  15. Personality, media preferences, and cultural participation

    NARCIS (Netherlands)

    Kraaykamp, Gerbert; Eijck, Koen van

    2005-01-01

    In this paper, the impact of the Big Five personality factors (extraversion, friendliness, conscientiousness, emotional stability, and openness) on media preferences (TV programs) and cultural participation (book reading and attending museums and concerts) was examined. The analyses were carried out

  16. Children and media: a cultural studies approach

    OpenAIRE

    D av i d B u c k i n g h a m

    2012-01-01

    Defying the traditional psychology understanding on what are the effects of the media and also the researches made on the subject, the present article offers an approach to the study of the relationship between children and the media, focusing mainly on television. We retrace the Cultural Studies perspective, although the researches from the Birmingham Centre have not worked over such age group.The work includes the model of the cultural circuit, it refuses to understand meaning as something ...

  17. Harnessing the landscape of microbial culture media to predict new organism–media pairings

    Science.gov (United States)

    Oberhardt, Matthew A.; Zarecki, Raphy; Gronow, Sabine; Lang, Elke; Klenk, Hans-Peter; Gophna, Uri; Ruppin, Eytan

    2015-01-01

    Culturing microorganisms is a critical step in understanding and utilizing microbial life. Here we map the landscape of existing culture media by extracting natural-language media recipes into a Known Media Database (KOMODO), which includes >18,000 strain–media combinations, >3300 media variants and compound concentrations (the entire collection of the Leibniz Institute DSMZ repository). Using KOMODO, we show that although media are usually tuned for individual strains using biologically common salts, trace metals and vitamins/cofactors are the most differentiating components between defined media of strains within a genus. We leverage KOMODO to predict new organism–media pairings using a transitivity property (74% growth in new in vitro experiments) and a phylogeny-based collaborative filtering tool (83% growth in new in vitro experiments and stronger growth on predicted well-scored versus poorly scored media). These resources are integrated into a web-based platform that predicts media given an organism's 16S rDNA sequence, facilitating future cultivation efforts. PMID:26460590

  18. Cell culture media impact on drug product solution stability.

    Science.gov (United States)

    Purdie, Jennifer L; Kowle, Ronald L; Langland, Amie L; Patel, Chetan N; Ouyang, Anli; Olson, Donald J

    2016-07-08

    To enable subcutaneous administration of monoclonal antibodies, drug product solutions are often needed at high concentrations. A significant risk associated with high drug product concentrations is an increase in aggregate level over the shelf-life dating period. While much work has been done to understand the impact of drug product formulation on aggregation, there is limited understanding of the link between cell culture process conditions and soluble aggregate growth in drug product. During cell culture process development, soluble aggregates are often measured at harvest using cell-free material purified by Protein A chromatography. In the work reported here, cell culture media components were evaluated with respect to their impact on aggregate levels in high concentration solution drug product during accelerated stability studies. Two components, cysteine and ferric ammonium citrate, were found to impact aggregate growth rates in our current media (version 1) leading to the development of new chemically defined media and concentrated feed formulations. The new version of media and associated concentrated feeds (version 2) were evaluated across four cell lines producing recombinant IgG4 monoclonal antibodies and a bispecific antibody. In all four cell lines, the version 2 media reduced aggregate growth over the course of a 12 week accelerated stability study compared with the version 1 media, although the degree to which aggregate growth decreased was cell line dependent. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:998-1008, 2016.

  19. Cultural Journalism and Cultural Critique in a changing Media Landscape

    DEFF Research Database (Denmark)

    Nørgaard Kristensen, Nete; From, Unni

    2015-01-01

    This special issue addresses a topic of journalism studies that has previously been somewhat neglected but which has gained increasing scholarly attention since the mid-2000s: the coverage and evaluation of art and culture, or what we term “cultural journalism and cultural critique.......” In this introduction, we highlight three issues that serve to frame the study of cultural journalism and cultural critique more generally and the eight articles of this special issue more specifically: (1) the constant challenge of demarcating cultural journalism and cultural critique, including the interrelations...... of “journalism” and “critique”; (2) the dialectic of globalisation’s cultural homogenisation, on the one hand, and the specificity of local/national cultures, on the other; and (3) the digital media landscape seen in terms of the need to rethink, perhaps even redefine cultural journalism and cultural critique...

  20. Culture of Scientific Information in Mass Media on the Internet

    Directory of Open Access Journals (Sweden)

    Arwa 'Isa al-Yasiry

    2006-12-01

    Full Text Available This research aims at evaluating the quality of scientific information culture that introduce it the Arabic mass media in the internet and how it covering the reality of Arabic scientific information by using analysis content method for these websites then we most be know how these websites treating with information culture considering information systems has input, output and mutual relations between the elements of this system that include the following three components: 1- External relations that connecting between the culture and the reality. 2- Internal elements for this system. 3- Infrastructures for this system that represented in the cultural policy, informational , information resources and human resources

  1. Media evolution and ‘epi-technic’ digital media: Media as cultural selection mechanisms

    DEFF Research Database (Denmark)

    Olesen, Mogens

    2016-01-01

    The explosive development of new digital media technologies is often described as a media evolution but hardly ever is the concept of ‘media evolution’ taken at face value. This article takes up that challenge by combining cultural evolution theories with medium theory. The article argues...... that biological selection mechanisms can provide an inroad into a new kind of historical and structural understanding of the relation between human culture and our technologies. In specific, human history is seen as a cultural evolution in which media technologies are the selection mechanisms....

  2. Valorization of crude glycerol and eggshell biowaste as media components for hydrogen production: A scale-up study using co-culture system.

    Science.gov (United States)

    Pachapur, Vinayak Laxman; Das, Ratul Kumar; Brar, Satinder Kaur; Le Bihan, Yann; Buelna, Gerardo

    2017-02-01

    The properties of eggshells (EGS) as neutralizing and immobilizing agent were investigated for hydrogen (H2) production using crude glycerol (CG) by co-culture system. Eggshells of different sizes and concentrations were used during batch and repeated-batch fermentation. For batch and repeated-batch fermentation, the maximum H2 production (36.53±0.53 and 41.16±0.95mmol/L, respectively) was obtained with the EGS size of 33μmglycerol utilization was obtained.

  3. A cultural evolution approach to digital media

    Directory of Open Access Journals (Sweden)

    Alberto Acerbi

    2016-12-01

    Full Text Available Digital media have today an enormous diffusion, and their influence on the behaviour of a vast part of the human population can hardly be underestimated. In this review I propose that cultural evolution theory, including both a sophisticated view of human behaviour and a methodological attitude to modelling and quantitative analysis, provides a useful framework to study the effects and the developments of media in the digital age. I will first give a general presentation of the cultural evolution framework, and I will then introduce this more specific research program with two illustrative topics.The first topic concerns how cultural transmission biases, that is, simple heuristics such as copy prestigious individuals or copy the majority, operate in the novel context of digital media. The existence of transmission biases is generally justified with their adaptivity in small-scale societies. How do they operate in an environment where, for example, prestigious individuals possess not-relevant skills, or popularity is explicitly quantified and advertised?The second aspect relates to fidelity of cultural transmission. Digitally-mediated interactions support cheap and immediate high-fidelity transmission, in opposition, for example, to oral traditions. How does this change the content that is more likely to spread? Overall, I suggest the usefulness of a long view to our contemporary digital environment, contextualised in cognitive science and cultural evolution theory, and I discuss how this perspective could help us to understand what is genuinely new and what is not.

  4. A Cultural Evolution Approach to Digital Media

    Science.gov (United States)

    Acerbi, Alberto

    2016-01-01

    Digital media have today an enormous diffusion, and their influence on the behavior of a vast part of the human population can hardly be underestimated. In this review I propose that cultural evolution theory, including both a sophisticated view of human behavior and a methodological attitude to modeling and quantitative analysis, provides a useful framework to study the effects and the developments of media in the digital age. I will first give a general presentation of the cultural evolution framework, and I will then introduce this more specific research program with two illustrative topics. The first topic concerns how cultural transmission biases, that is, simple heuristics such as “copy prestigious individuals” or “copy the majority,” operate in the novel context of digital media. The existence of transmission biases is generally justified with their adaptivity in small-scale societies. How do they operate in an environment where, for example, prestigious individuals possess not-relevant skills, or popularity is explicitly quantified and advertised? The second aspect relates to fidelity of cultural transmission. Digitally-mediated interactions support cheap and immediate high-fidelity transmission, in opposition, for example, to oral traditions. How does this change the content that is more likely to spread? Overall, I suggest the usefulness of a “long view” to our contemporary digital environment, contextualized in cognitive science and cultural evolution theory, and I discuss how this perspective could help us to understand what is genuinely new and what is not. PMID:28018200

  5. A Cultural Evolution Approach to Digital Media.

    Science.gov (United States)

    Acerbi, Alberto

    2016-01-01

    Digital media have today an enormous diffusion, and their influence on the behavior of a vast part of the human population can hardly be underestimated. In this review I propose that cultural evolution theory, including both a sophisticated view of human behavior and a methodological attitude to modeling and quantitative analysis, provides a useful framework to study the effects and the developments of media in the digital age. I will first give a general presentation of the cultural evolution framework, and I will then introduce this more specific research program with two illustrative topics. The first topic concerns how cultural transmission biases, that is, simple heuristics such as "copy prestigious individuals" or "copy the majority," operate in the novel context of digital media. The existence of transmission biases is generally justified with their adaptivity in small-scale societies. How do they operate in an environment where, for example, prestigious individuals possess not-relevant skills, or popularity is explicitly quantified and advertised? The second aspect relates to fidelity of cultural transmission. Digitally-mediated interactions support cheap and immediate high-fidelity transmission, in opposition, for example, to oral traditions. How does this change the content that is more likely to spread? Overall, I suggest the usefulness of a "long view" to our contemporary digital environment, contextualized in cognitive science and cultural evolution theory, and I discuss how this perspective could help us to understand what is genuinely new and what is not.

  6. 21 CFR 866.2450 - Supplement for culture media.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Supplement for culture media. 866.2450 Section 866...) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2450 Supplement for culture media. (a) Identification. A supplement for culture media is a device, such as a vitamin or sugar...

  7. Harnessing the landscape of microbial culture media to predict new organism–media pairings

    OpenAIRE

    Oberhardt, Matthew A.; Zarecki, Raphy; Gronow, Sabine; Lang, Elke; Klenk, Hans-Peter; Gophna, Uri; Ruppin, Eytan

    2015-01-01

    Culturing microorganisms is a critical step in understanding and utilizing microbial life. Here we map the landscape of existing culture media by extracting natural-language media recipes into a Known Media Database (KOMODO), which includes >18,000 strain–media combinations, >3300 media variants and compound concentrations (the entire collection of the Leibniz Institute DSMZ repository). Using KOMODO, we show that although media are usually tuned for individual strains using biologically comm...

  8. Media Culture 2020: Collaborative Teaching and Blended Learning Using Social Media and Cloud-Based Technologies

    Science.gov (United States)

    Vickers, Richard; Field, James; Melakoski, Cai

    2015-01-01

    In 2013 five universities from across Europe undertook an innovative project "Media Culture 2020", combining skills and forces to develop new practices that would face the challenge of the convergence of digital media, taking full advantage of social media and cloud-based technologies. The aim of the Media Culture 2020 project was to…

  9. Association of Various Components of Media Literacy and Adolescent Smoking

    Science.gov (United States)

    Primack, Brian A.; Hobbs, Renee

    2009-01-01

    Objective: To determine which specific aspects of media literacy were most strongly associated with smoking outcomes. Methods: Students at a public high school responded to cross-sectional survey items measuring smoking outcomes, components of media literacy, and other variables. Results: Of the 1211 participants, 19% were current smokers (N =…

  10. Media Literacy: A Central Component of Democratic Citizenship

    Science.gov (United States)

    Burroughs, Susie; Brocato, Kay; Hopper, Peggy F.; Sanders, Angela

    2009-01-01

    Educators from Europe, Latin America, and the United States convened to explore issues inherent in democratic citizenship. Media literacy, a central component of democratic citizenship, was studied in depth. Data from the camp were examined for evidence of the participants' understandings of media literacy and how it might be taught. Results…

  11. Spatio temporal media components for neurofeedback

    DEFF Research Database (Denmark)

    Jensen, Camilla Birgitte Falk; Petersen, Michael Kai; Larsen, Jakob Eg

    2013-01-01

    a strong influence on the ability to control brain activity. Therefore, identifying the different interface components and exploring their individual effects might be key for constructing new interfaces that support more efficient neurofeedback training. We discuss experiments involving two different...... designs of neurofeedback interfaces and suggest further research to clarify the influence of different audiovisual components and temporal settings on neurofeedback effect....

  12. Transculturality as a Perspective: Researching Media Cultures Comparatively

    Directory of Open Access Journals (Sweden)

    Andreas Hepp

    2009-01-01

    Full Text Available Most of the research on media cultures operates in a "national-territorial" frame. Media cultures are considered as national cultures and other forms of media culture (for example professional journalism cultures, diasporas, celebrity cultures etc. are not investigated in their "deterritorial" character. But it is exactly such deterritorial forms of media culture that are gaining relevance with the ongoing pace of media globalization: they therefore have to be placed in the focus of comparative media and communication research. Starting with this consideration, the article develops a transcultural perspective on researching media cultures. Within this perspective it becomes possible to conduct comparative research on (territorial national media cultures as well as on other (deterritorial forms of present media cultures, as this approach moves the processes of cultural construction and articulation into the focus of analysis. To arrive at a better understanding of this approach, "media cultures" are defined as translocal phenomena in their territorial as well as their deterritorial relations. Based on this, the "semantics" of a transcultural research perspective are outlined, which then makes it possible to formulate practical principles for carrying out comparative qualitative research within this framework. URN: urn:nbn:de:0114-fqs0901267

  13. Brand Identity, Adaptation, and Media Franchise Culture

    Directory of Open Access Journals (Sweden)

    Marazi Katerina

    2014-12-01

    Full Text Available In spite of the noticeable practices within the field of Adaptation, Adaptation theory seems to be lagging behind whilst perpetuating various fallacies. Geoffrey Wagner’s types of Adaptation and Kamilla Elliott’s proposed concepts for examining adaptations have proved useful but due to their general applicability they seem to perpetuate the fallacies existing within the field of Adaptation. This article will propose a context-specific concept pertaining to Media Franchise Culture for the purpose of examining Adaptations and re-assessing long-held debates concerning the Original, the Content/Form debate and Fidelity issues that cater to the twelve fallacies discussed by Thomas Leitch.

  14. EFFECTS OF CULTIVATION MEDIA COMPONENTS ON BIOSURFACTANT AND PIGMENT PRODUCTION FROM Pseudomonas aeruginosa PAO1

    Directory of Open Access Journals (Sweden)

    Subhasish Das

    2015-06-01

    Full Text Available AbstractBiosurfactant and pigment production by P. aeruginosa have been known to be associated with its selfish growth and colonization. However, according to recent studies these products can be exploited for human benefits. In the present work the effects of culture media components on yield of these two products from P. aeruginosa PAO1 have been studied with statistical screening design experiments. Biosurfactant yield was found to be increased by two times in a modified medium. This study will help in further modifying the media composition for cheaper media development, kinetic modelling and fermentation strategy development.

  15. Information Feedback and Mass Media Effects in Cultural Dynamics

    OpenAIRE

    González-Avella, Juan Carlos; Cosenza, Mario G.; Klemm, Konstantin; Eguíluz, Víctor M; San Miguel, Maxi

    2007-01-01

    We study the effects of different forms of information feedback associated with mass media on an agent-agent based model of the dynamics of cultural dissemination. In addition to some processes previously considered, we also examine a model of local mass media influence in cultural dynamics. Two mechanisms of information feedback are investigated: (i) direct mass media influence, where local or global mass media act as an additional element in the network of interactions of each agent, and (i...

  16. Information feedback and mass media effects in cultural dynamics

    OpenAIRE

    González-Avella, Juan Carlos; Mario G. Cosenza; Klemm, Konstantin; Eguíluz, Víctor M.; San Miguel, Maxi

    2007-01-01

    We study the effects of different forms of information feedback associated with mass media on an agent-agent based model of the dynamics of cultural dissemination. In addition to some processes previously considered, we also examine a model of local mass media influence in cultural dynamics. Two mechanisms of information feedback are investigated: (i) direct mass media influence, where local or global mass media act as an additional element in the network of interactions of each agent, and (i...

  17. RELIGIOUS COMMUNICATION IN THE CONTEXT OF CULTURE MEDIA

    OpenAIRE

    Petrushkevych, Maria S.

    2014-01-01

    Purpose. The aim of the work is to determine the features of media culture that bind it with mass culture and mass communications and have the most significant effect on the general principles of the religious mass communication. In addition, the objective is to identify the skills system and traits of mass human that are necessary for using media culture. Methodology. The methodological basis is related to structuring, analytical analysis and synthesis of media features; highlighting phenome...

  18. A fluorescence anisotropy method for measuring protein concentration in complex cell culture media.

    Science.gov (United States)

    Groza, Radu Constantin; Calvet, Amandine; Ryder, Alan G

    2014-04-22

    The rapid, quantitative analysis of the complex cell culture media used in biopharmaceutical manufacturing is of critical importance. Requirements for cell culture media composition profiling, or changes in specific analyte concentrations (e.g. amino acids in the media or product protein in the bioprocess broth) often necessitate the use of complicated analytical methods and extensive sample handling. Rapid spectroscopic methods like multi-dimensional fluorescence (MDF) spectroscopy have been successfully applied for the routine determination of compositional changes in cell culture media and bioprocess broths. Quantifying macromolecules in cell culture media is a specific challenge as there is a need to implement measurements rapidly on the prepared media. However, the use of standard fluorescence spectroscopy is complicated by the emission overlap from many media components. Here, we demonstrate how combining anisotropy measurements with standard total synchronous fluorescence spectroscopy (TSFS) provides a rapid, accurate quantitation method for cell culture media. Anisotropy provides emission resolution between large and small fluorophores while TSFS provides a robust measurement space. Model cell culture media was prepared using yeastolate (2.5 mg mL(-1)) spiked with bovine serum albumin (0 to 5 mg mL(-1)). Using this method, protein emission is clearly discriminated from background yeastolate emission, allowing for accurate bovine serum albumin (BSA) quantification over a 0.1 to 4.0 mg mL(-1) range with a limit of detection (LOD) of 13.8 μg mL(-1). Copyright © 2014. Published by Elsevier B.V.

  19. Cross-Cultural Identity and Media Globalization, Localization

    Institute of Scientific and Technical Information of China (English)

    ZHANG Hai-yan

    2015-01-01

    Recent years witness the great concerns and anxieties from an increasing number of researchers upon the issue of media globalization and culture identity, or rather homogenization and heterogeneity. There, however, exists a harmonious relationship be⁃tween media globalization and culture identity, rather than a contradictive one. Media globalization can effectively represent audi⁃ence’s culture identity in its process of localization. In sense of culture, reasons to media localization and measures to achieve lo⁃calization will be discussed from the commercial, social and practical perspectives.

  20. Media and Cultural Industries: a Socioeconomic Approach

    Directory of Open Access Journals (Sweden)

    Bernard Miège

    2008-05-01

    Full Text Available This article reviews the discussion that initiated in the 70’s about the relationship between communication and information phenomena, and decisions in the economic field. This discussion, according to Miège, has been undertaken from different perspectives that have placed economy and technology at the core of the analysis. The author proposes to study these phenomena through an interdisciplinary methodology, based on the theories of cultural industries and thepolitical economy of communication. Miège argues that with industrialization of media contents, consumer product access is no longer direct and products may be available without any cost to the consumer, since the cost of informational and cultural products is paid through advertising. However, this new environment creates certain problems, such as regulating the sale of these products, turning them and their symbolic content as marketable goods or hiring intellectual and artistic workers under an unregulated framework. He also discusses a double economic operation: the sale of products to publicists, and the sale of the same products by the publicists according to the market demand. The last part of the article is an analysis made by the author on the consequences that economic changes might have on cultural industries, because of their current need to keep cooperation relationships with technological industries, as well as connections with large financial groups.

  1. Main Quality Attributes of Monoclonal Antibodies and Effect of Cell Culture Components

    Science.gov (United States)

    Torkashvand, Fatemeh; Vaziri, Behrouz

    2017-05-01

    The culture media optimization is an inevitable part of upstream process development in therapeutic monoclonal antibodies (mAbs) production. The quality by design (QbD) approach defines the assured quality of the final product through the development stage. An important step in QbD is determination of the main quality attributes. During the media optimization, some of the main quality attributes such as glycosylation pattern, charge variants, aggregates, and low-molecular-weight species, could be significantly altered. Here, we provide an overview of how cell culture medium components affects the main quality attributes of the mAbs. Knowing the relationship between the culture media components and the main quality attributes could be successfully utilized for a rational optimization of mammalian cell culture media for industrial mAbs production.

  2. Culture of Chlorella ellipsoidea in different culture media

    Directory of Open Access Journals (Sweden)

    MM Mohshina

    2017-06-01

    Full Text Available An experiment of algal culture was conducted in natural light and temperature conditions at a balcony of a room at the 2nd floor of Fisheries Faculty Building facing the north. The experiment was done to evaluate the growth of Chlorella ellipsoidea in four different media, viz, medium I (inorganic, medium II (organic, whole pulse powder extract, medium III (organic, whole lentil powder extract and medium IV (organic, whole gram powder extract under natural environment conditions during January-June, 2015. Growth rates of the algal species in four different media were found not significantly different. The alga, C. ellipsoidea attained maximum cell density of 28.89×106 cell ml-1 in the 15th day in medium I, of 30.69×106 cell ml-1 in the 13th day in medium II, of 26.18×106 cell ml-1 in the 15th day in medium III and of 21.12×106 cell ml-1 in the 13th day in medium IV. The ranges of air temperature, water temperature and light intensity were 21°C to 38°C, 23°C to 36°C and 2.28×103to 9.60×103 Lux respectively during the culture period. The average sunshine period was 5.87±2.82 hrs. Total alkalinity, free CO2, pH , NO3-N and PO4-P of algal culture media I, II, III and IV were 128, 540, 554 and 322 mgL-1; 32, 162, 102, 70 mgL-1; 7.4, 8, 7.9 and 7.9; 180, 36.6, 62.4 and 150 mgL-1, and 25.2, 48.2, 42.4 and 45.6 mgL-1, respectively. According to ANOVA of cell densities of cultures of C. ellipsoidea under treatments are not significantly different (F=1.441077. It is clear that differences between them are not significant i.e. mean algal cell densities are more or less same as differences between treatments are less than 20%.

  3. Colorimetric pH measurement of animal cell culture media.

    Science.gov (United States)

    Jang, Juno; Moon, Soo-Jin; Hong, Sung-Hwan; Kim, Ik-Hwan

    2010-11-01

    Most animal cell culture media can be buffered using bicarbonate and high pressure CO(2) in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO(2) pressure between the culture media and the atmosphere. Therefore, it is important to measure the exact pH of the culture media in an intact closed system. In this study, a pH measurement method was developed using visible light. The pH was calculated from light absorbance by the cells and by the culture media. This method was successfully applied to both suspension and anchorage-dependent cell cultures.

  4. Social media is a necessary component of surgery practice.

    Science.gov (United States)

    Steele, Scott R; Arshad, Seyed; Bush, Ruth; Dasani, Serena; Cologne, Kyle; Bleier, Joshua I S; Raphaeli, Tal; Kelz, Rachel R

    2015-09-01

    Social media is a necessary component of the practice of surgery. Each surgeon must embrace the power and potential of social media and serve as a guide or content expert for patients and other health care providers to facilitate and share responsible use of the various media available. Social media facilitates rapid communication of information not only across providers but also between patients and providers. The power of social media has the potential to improve consultation and collaboration, facilitate patient education, and expand research efforts; moreover, by harnessing its potential, the appropriate use of many of the avenues of social media also can be used to disseminate campaigns to increase disease awareness and communicate new research findings and best-practice guidelines. Because its reach is so broad within as well as outside the censorship of medical experts, professional oversight and engagement is required to maximize responsible use. Staying consistent with our history of surgery, rich in innovation and technologic advancement, surgeons must get to the front of this evolving field and direct the path of social media as it applies to the practice of surgery rather than take a passive role. Published by Elsevier Inc.

  5. Overcoming Impossible Bodies: Using Media Literacy to Challenge Popular Culture.

    Science.gov (United States)

    Graydon, Shari

    1997-01-01

    Media education can be taught by analyzing the ways popular media represent the sexes. Discusses stereotyped gender images in popular culture and outlines classroom activities investigating modeling poses, images of ideal and successful males and females, gender sensitive language, sex role portrayal, and violence for a media literacy unit using…

  6. Use of gamma radiation for preparation of nutrient culture media

    Energy Technology Data Exchange (ETDEWEB)

    Speranskaya, I.D.; Tumanyan, M.A.; Mironova, L.L.

    1977-01-01

    A technique was developed for sterilization of nutrient culture media using ..gamma..-radiation. For this purpose, dry preparations were exposed to 3 to 6 Mrad radiation, then dissolved in sterile distilled water. The quality of media and solutions thus obtained is as good as that of preparations sterilized by filtration. The advantage of the proposed sterilization method is that liquid media can be rapidly prepared and dry sterile media can be stored at room temperature for long periods of time.

  7. Greening the Media Literacy Ecosystem: Situating Media Literacy for Green Cultural Citizenship

    Science.gov (United States)

    Lopez, Antonio R.

    2013-01-01

    Media literacy is touted as a necessary life skill for cultural citizenship, yet as it is generally practiced there is little engagement with sustainability issues. In order to gain insights into why this is the case, this research investigated how media literacy practitioners use metaphors to frame both the role of media education in the world…

  8. Karl Marx and the Study of Media and Culture Today

    OpenAIRE

    Fuchs, Christian

    2014-01-01

    The task of this paper discusses the role of Marx in analysing media, communica-tion and culture today. An analysis of three contemporary Cultural Studies works – Lawrence Grossberg’s monograph Cultural Studies in the Future Tense, John Hartley’s monograph Digital Futures for Cultural and Media Studies and Paul Smith’s edited volume The Renewal of Cultural Studies – shows that there is an agreement that the economy needs to be taken more into account by Cultural Studies, but disagreement on w...

  9. Cultural Studies and Media Ecology: Meyrowitz's Medium Theory and Carey's Cultural Studies.

    Science.gov (United States)

    Flayhan, Donna P.

    2001-01-01

    Examines work of two communication and media studies scholars, Joshua Meyrowitz and James Carey. Suggests their studies represent media ecology with analyses of the dynamic interaction between communication, consciousness, and culture. Highlights how their work embodies a North American cultural studies approach to media studies (moving away from…

  10. Cultural Studies and Media Ecology: Meyrowitz's Medium Theory and Carey's Cultural Studies.

    Science.gov (United States)

    Flayhan, Donna P.

    2001-01-01

    Examines work of two communication and media studies scholars, Joshua Meyrowitz and James Carey. Suggests their studies represent media ecology with analyses of the dynamic interaction between communication, consciousness, and culture. Highlights how their work embodies a North American cultural studies approach to media studies (moving away from…

  11. Karl Marx and the Study of Media and Culture Today

    Directory of Open Access Journals (Sweden)

    Christian Fuchs

    2014-02-01

    Full Text Available The task of this paper discusses the role of Marx in analysing media, communica-tion and culture today. An analysis of three contemporary Cultural Studies works – Lawrence Grossberg’s monograph Cultural Studies in the Future Tense, John Hartley’s monograph Digital Futures for Cultural and Media Studies and Paul Smith’s edited volume The Renewal of Cultural Studies – shows that there is an agreement that the economy needs to be taken more into account by Cultural Studies, but disagreement on which approach should be taken and what the role of Karl Marx’s works shall be. The paper argues that Marx’s labour theory of value is especially important for critically analysing the media, culture and communica-tion. Labour is still a blind spot of the study of culture and the media, although this situation is slowly improving. It is maintained that the turn away from Marx in Cultural and Media Studies was a profound mistake that should be reverted. Only an engagement with Marx can make Cultural and Media Studies topical, politically relevant, practical and critical, in the current times of global crisis and resurgent critique.

  12. Media, cultural diversity and globalization: challenges and opportunities.

    Science.gov (United States)

    Zayani, Mohamed

    2011-01-01

    This paper explores the role media play in safeguarding cultural diversity, promoting cultural dialogue, facilitating the exercise of cultural rights,fostering cultural understanding and cultivating intercultural citizenship in the age of globalization. The paper highlights several interconnected leverage points: media content, practices, processes, ownership, education, structures, and policies. It argues that fostering cultural diversity in and through the media can go a long way toward bringing a civic discourse which favors tolerance and facilitates co-existence. It can contribute to the breaking down of cultural barriers, the initiation of cultural dialogues, the empowerment of marginalized groups, and the practice of good governance. At the same time, this paper argues, the celebration of difference does not preclude the valuation of a common cultural core or a common humanity which brings people together in spite of their differences.

  13. Data Mining Cultural Aspects of Social Media Marketing

    OpenAIRE

    Hochreiter, Ronald; Waldhauser, Christoph

    2014-01-01

    For marketing to function in a globalized world it must respect a diverse set of local cultures. With marketing efforts extending to social media platforms, the crossing of cultural boundaries can happen in an instant. In this paper we examine how culture influences the popularity of marketing messages in social media platforms. Text mining, automated translation and sentiment analysis contribute largely to our research. From our analysis of 400 posts on the localized Google+ pages of German ...

  14. MEDIA COMMUNICATION: UNIFORMITY OR CULTURAL DIVERSITY?

    OpenAIRE

    Simona Wist

    2009-01-01

    Thanks to the extent acquired in the recent decades, especially after the development of new communication technologies and the explosion of mass media such as press, radio, Internet and television, media communication is attaining the status of supreme power in the social hierarchy in Europe and worldwide, and its effects are felt widely by individuals.Of all mass media, television is one that is individualized by its symbolic power, having a noticeable influence, direct and undisputed upon ...

  15. A micropolar mixture theory of multi-component porous media

    Institute of Scientific and Technical Information of China (English)

    Lu HUANG; Cheng-gang ZHAO

    2009-01-01

    A mixture theory is developed for multi-component micropolar porous media with a combination of the hybrid mixture theory and the micropolar continuum theory.The system is modeled as multi-component micropolar elastic solids saturated with multicomponent micropolar viscous fluids. Balance equations are given through the mixture theory. Constitutive equations are developed based on the second law of thermodynamics and constitutive assumptions. Taking account of compressibility of solid phases,the volume fraction of fluid as an independent state variable is introduced in the free energy function,and the dynamic compatibility condition is obtained to restrict the change of pressure difference on the solid-fluid interface. The constructed constitutive equations are used to close the field equations. The linear field equations are obtained using a linearization procedure,and the micropolar thermo-hydro-mechanical component transport model is established. This model can be applied to practical problems,such as contaminant,drug,and pesticide transport. When the proposed model is supposed to be porous media,and both fluid and solid are single-component,it will almost agree with Eringen's model.

  16. Health communication: a media and cultural studies approach

    National Research Council Canada - National Science Library

    Lewis, Belinda; Lewis, Jeff

    2015-01-01

    "This book is about communicating for health and social change. With a clear focus on public health and health promotion practice, it provides a unique introduction to media and cultural studies perspectives on health communication...

  17. PERSON AS A MEDIA PHENOMENON OF SOCIO-CULTURAL PROCESSES

    National Research Council Canada - National Science Library

    M. M. GUKASOVA

    2016-01-01

    The article discusses a reference concept of a person, a media phenomenon of socio-cultural process, it also finds out problems of personalization and their salvation in anthropocentric and socio-centric aspects...

  18. Queer Girls and Popular Culture: Reading, Resisting, and Creating Media

    Science.gov (United States)

    Blackburn, Mollie V.

    2010-01-01

    This article reviews Driver's monograph, "Queer Girls and Popular Culture: Reading, Resisting, and Creating Media," reporting on queer girls' active engagement with television characters, films, lesbian magazines, online communities, and music. She explores the consequences of their engagements with these media on their lives and their…

  19. Mediatization: Theorizing the Interplay Between Media, Culture, and Society

    DEFF Research Database (Denmark)

    Hepp, Andreas; Hjarvard, Stig; Lundby, Knut

    2015-01-01

    with the complex relationship between changes in media and communication on the one hand and changes in various fields of culture and society on the other. We conclude that the emergence of the concept of mediatization is part of a paradigmatic shift within media and communication research....

  20. Queer Girls and Popular Culture: Reading, Resisting, and Creating Media

    Science.gov (United States)

    Blackburn, Mollie V.

    2010-01-01

    This article reviews Driver's monograph, "Queer Girls and Popular Culture: Reading, Resisting, and Creating Media," reporting on queer girls' active engagement with television characters, films, lesbian magazines, online communities, and music. She explores the consequences of their engagements with these media on their lives and their…

  1. Cultural Genocide through Mainstream Media: A Brief Critical Analysis

    Science.gov (United States)

    Olague, Rubén; Ekiaka Nzai, Valentin

    2014-01-01

    While the U.S. mainstream media continues to exercise its right of way in the American landscape, the predominant culture faces a population and popularity decrease. Diversity is slowly finding a perennial nest for growth, although minorities are still being shelled by mainstream media that consciously and unconsciously make the attack a priority…

  2. Reengineering the Innovation Culture through Social media Crowdsourcing

    DEFF Research Database (Denmark)

    Scupola, Ada; Nicolajsen, Hanne Westh

    2012-01-01

    In this article we investigate how social media-based crowdsourcing systems can be used to reengineer the innovation culture in an organization. Based on a case study of a large engineering consultancy’s use of a social media crowdsourcing system we investigate the impact on the organizations...... innovation culture using theory on organizational culture and crowdsourcing. The analysis shows that the organizational crowdsourcing event has supported an innovation culture change in the case company towards a more including approach to innovation; creating a new and different awareness of innovation...

  3. Dynamics of two-component membranes surrounded by viscoelastic media.

    Science.gov (United States)

    Komura, Shigeyuki; Yasuda, Kento; Okamoto, Ryuichi

    2015-11-01

    We discuss the dynamics of two-component fluid membranes which are surrounded by viscoelastic media. We assume that membrane-embedded proteins can diffuse laterally and induce a local membrane curvature. The mean squared displacement of a tagged membrane segment is obtained as a generalized Einstein relation. When the elasticity of the surrounding media obeys a power-law behavior in frequency, an anomalous diffusion of the membrane segment is predicted. We also consider the situation where the proteins generate active non-equilibrium forces. The generalized Einstein relation is further modified by an effective temperature that depends on the force dipole energy. The obtained generalized Einstein relations are useful for membrane microrheology experiments.

  4. RELIGIOUS COMMUNICATION IN THE CONTEXT OF CULTURE MEDIA

    Directory of Open Access Journals (Sweden)

    Maria S. Petrushkevych

    2014-06-01

    Full Text Available Purpose. The aim of the work is to determine the features of media culture that bind it with mass culture and mass communications and have the most significant effect on the general principles of the religious mass communication. In addition, the objective is to identify the skills system and traits of mass human that are necessary for using media culture. Methodology. The methodological basis is related to structuring, analytical analysis and synthesis of media features; highlighting phenomena that illustrate modern communicative situation; characteristics of media trends influence for the specific functioning of religious communication. Scientific novelty. Main part of the work is devoted to the analysis of the progressive media culture, mass-media and their main features, design of religious communication in this culture. Media gradually form the appearance of religious communication quietly, especially the mass one, they adapt the modern religious discourse to rates of transfer and perception of information. Modern believer gets a lot of different kinds of religious information, on any subject, any explanation of the religious question, with respect to any religion. Such volume of religious information and the speed with which a person receives it, does not usually make it religious or spiritually advanced, but only informed. Spiritual perfection and religious development, religious communication is possible only when the customer is aware of media culture and way of seeing the ultimate goal of such communications using the Mass Media. So far these mechanisms are perfectly designed in traditional religious communication. Phenomena, that reflects the dramatic changes in the communicative environment are: mediatization of body and mind, the new practice of processing / reading information, the phenomenon of simultaneous perception of a large number of information channels – similar or different. Features of media culture that connect it with

  5. 77 FR 68829 - Certain Electronic Digital Media Devices and Components Thereof; Notice of Request for Statements...

    Science.gov (United States)

    2012-11-16

    ... From the Federal Register Online via the Government Publishing Office INTERNATIONAL TRADE COMMISSION Certain Electronic Digital Media Devices and Components Thereof; Notice of Request for Statements... limited exclusion order against certain electronic digital media devices and components thereof...

  6. 78 FR 6130 - Certain Electronic Digital Media Devices and Components Thereof: Commission Determination To...

    Science.gov (United States)

    2013-01-29

    ... From the Federal Register Online via the Government Publishing Office INTERNATIONAL TRADE COMMISSION Certain Electronic Digital Media Devices and Components Thereof: Commission Determination To... certain electronic digital media devices and components thereof by reason of infringement of...

  7. 21 CFR 876.5885 - Tissue culture media for human ex vivo tissue and cell culture processing applications.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Tissue culture media for human ex vivo tissue and... DEVICES Therapeutic Devices § 876.5885 Tissue culture media for human ex vivo tissue and cell culture processing applications. (a) Identification. Tissue culture media for human ex vivo tissue and cell culture...

  8. Media literacy and the challenges of contemporary media culture: on savvy viewers and critical apathy

    NARCIS (Netherlands)

    Teurlings, J.

    2010-01-01

    This article aims to make a contribution to the media literacy movement by focusing on the debate between liberal and more radical approaches. It argues that the media literacy movement is fighting a battle that is already partly won, but that contemporary popular culture has moved into a terrain th

  9. 76 FR 47610 - Certain Electronic Digital Media Devices and Components Thereof; Notice of Institution of...

    Science.gov (United States)

    2011-08-05

    ... COMMISSION Certain Electronic Digital Media Devices and Components Thereof; Notice of Institution of... of certain electronic digital media devices and components thereof by reason of infringement of... electronic digital media devices and components thereof ] that infringe one or more of claims 1, 3-6, and...

  10. Survival and function of phagocytes in blood culture media

    DEFF Research Database (Denmark)

    Fischer, T K; Prag, J; Kharazmi, A

    1999-01-01

    The survival and function of human phagocytes in sterile aerobic and anaerobic blood culture media were investigated using neutrophil morphology, white blood cell count in a haemoanalyser, flow cytometry, oxidative burst response, and bactericidal effect in Colorbact and Septi-Chek blood culture...

  11. Mass Society/Culture/Media: An Eclectic Approach.

    Science.gov (United States)

    Clavner, Jerry B.

    Instructors of courses in mass society, culture, and communication start out facing three types of difficulties: the historical orientation of learning, the parochialism of various disciplines, and negative intellectually elitist attitudes toward mass culture/media. Added to these problems is the fact that many instructors have little or no…

  12. Peace process in cultural conflict: The role of the media

    Directory of Open Access Journals (Sweden)

    Dov Shinar

    2003-04-01

    Full Text Available This article explores (1 the cultural nature of the Palestinian-Israeli conflict; (2 the "intractability" of cultural conflicts; (3 conflict management models: reconciliation/"end-of-conflict" versus "conflict transformation" and their relation to cultural conflict; (4 the serious consequences of the wrong matching of models and conflicts, such as using the reconciliation model in cultural conflict; (5 the changing role of the media in international relations, and their contribution to the "crisis of expectations" that came to fruition in September 2000, with the eruption of the Intifada; (6 the possibility of the media contributing to peace processes; and (7 implications of the media adoption of the conflict transformation model. The premises are that, unlike other violent confrontations, the Middle Eastern conflict is fundamentally cultural, particularly in its Palestinian-Israeli version; that cultural conflicts are "intractable" (Lederach, 1998; Burgess&Burgess, 1996; Kraybill, 1995, in the sense that they are very difficult, perhaps impossible to resolve; that reconciliation is not the only possible or desirable outcome of conflict: transformation (Vayrynen, 1991 is another viable option; that mistaken interpretations of conflict-resolution strategies can lead to "crises of expectations" in policy-making, in the media, and in public opinion; and that the media can play important roles in these processes.

  13. Suitability of peracetic acid for sterilization of media for mycoplasma cultures.

    Science.gov (United States)

    Wutzler, P; Sprössig, M; Peterseim, H

    1975-01-01

    The utility of peracetic acid for sterilization of serum and yeast extract additions to mycoplasma medium was studied by culturing six Mycoplasma species. Culture media containing additions that had been sterilized with peracetic acid proved to be as good as filtered components. The use of 0.05 to 0.1% peracetic acid is recommended to sterilize the serum and yeast extract additions since savings in time and equipment can be accomplished. PMID:1100656

  14. Stability of resazurin in buffers and mammalian cell culture media

    DEFF Research Database (Denmark)

    Rasmussen, Eva; Nicolaisen, G.M.

    1999-01-01

    The utility of a ferricyanide/ferrocyanide system used in the AlamarBlue(TM) (Serotec, Oxford, UK) vital. dye to inhibit the reduction of resazurin by mammalian cell culture media is questioned. Resazurin was found to be relatively stable when dissolved in phosphate-buffered saline (PBS). The use...... of HEPES resulted in a huge immediate dye reduction, which was significantly enhanced by exposure to diffuse light from fluorescent tubes in the laboratory 8 h per day. The reduction of resazurin by various cell culture media was time and temperature dependent, and it was significantly enhanced......'s nutrient mixture F-10 and F-12. Fetal calf serum (5-20%) slightly decreased resazurin reduction during the first 2 days of incubation. The reduction of resazurin by mammalian cell culture media do not appear to be problematic under normal culture conditions, and it is primarily dependent upon the presence...

  15. Media organizational culture and innovative performance

    NARCIS (Netherlands)

    R. van der Wurff; M. Leenders

    2008-01-01

    Innovation is an important dimension of company performance, especially in the media industry where yesterday’s news is old news, audience tastes are shifting unexpectedly, and technology is changing at the proverbial Internet speed. In this paper, we discuss innovative performance in relation to th

  16. Cultural Heritage Meets Mobile Media - and New Games Emerge

    DEFF Research Database (Denmark)

    Jensen, Jens F.

    The paper describes and evaluates a recent project in Aalborg, Denmark, dealing with the communication of cultural heritage and industrial culture to young people via their own preferred media platform: mobile phones. The communication was based on the new cultural genre: Alternative Reality Games......, a method that - so to speak - writes the player into the story and history, and a method that because of the narrative form is especially well-suited to support coherences and coherent stories....

  17. Culture and Language Teaching through Media

    Science.gov (United States)

    Tanriverdi, Belgin; Apak, Ozlem

    2008-01-01

    The topic of teaching and learning culture has been a matter of considerable interest to language educators and much has been written about the role of culture in foreign language instruction over the past two decades. ESL students whose success in a new environment is conditioned not only by their mastery of the new language, but also, and…

  18. Cultural Journalism and Cultural Critique in a changing Media Landscape

    DEFF Research Database (Denmark)

    Nørgaard Kristensen, Nete; From, Unni

    2015-01-01

    This special issue addresses a topic of journalism studies that has previously been somewhat neglected but which has gained increasing scholarly attention since the mid-2000s: the coverage and evaluation of art and culture, or what we term “cultural journalism and cultural critique...

  19. Spreadable media: Creating value and meaning in a networked culture

    Directory of Open Access Journals (Sweden)

    Moderated by Louisa Stein

    2014-09-01

    Full Text Available Online Roundtable on Spreadable Media, by Henry Jenkins, Sam Ford, and Joshua Green, with participants Paul Booth, Kristina Busse, Melissa Click, Sam Ford, Henry Jenkins, Xiaochang Li, and Sharon Ross. Section 1 first published as the article "Spreadable Media: Creating Value and Meaning in a Networked Culture", by Louisa Stein, from Cinema Journal Volume 53 Issue 3, pp152-177. Copyright 2014 by The University of Texas Press. All rights reserved.

  20. The Components of the Organizational Culture

    Directory of Open Access Journals (Sweden)

    Cornelia Elena TUREAC

    2005-10-01

    Full Text Available Knowing that the organizational culture functions constitute the base of notification and capitalization of the major importance, which ishaving for every company, regardless the domain of activity, dimension, economic potential or belonging to a certain national culture. Theimportance of the organizational culture is consisting of its functions, the importance is manifesting trough some concrete elements the companylevel and not only. The organizational culture is allowing the identification and the description of some numerous situations and real facts, of humannature from the company’s life, with major implications over the development and the activity results, that weren’t taken into consideration in themanagerial classic leading. This explains why the elements referring to the organizational culture were accepted very fast by numerous companies’managers from the developed countries.

  1. Youth culture, media and sexuality: What could faith communities contribute?

    Directory of Open Access Journals (Sweden)

    Anita Cloete

    2012-02-01

    Full Text Available This article provided an overview of youth culture and how the media shapes youth culture today. Its specific aim was to focus on the access to sexual content that the different forms of media provide and the possible effect that they have on youth culture today. The sexual development of teenagers is one of the most important areas of their journey into adulthood and can easily be influenced by media messages on sex and sexuality. As such, the sexual behaviour of teenagers mostly seems to demonstrate a misconception on sex and sexuality. The author argued that sex and sexuality can also be viewed as theological issues and concluded by offering a few suggestions on how faith communities can become a more relevant and effective partner in fostering a theological understanding of sex and sexuality, especially to the youth.

  2. Hermeneutic Analysis of the Cultural Context of the Functioning of Media in Society and Media Texts on Media Literacy Education Classes

    Directory of Open Access Journals (Sweden)

    Alexander Fedorov

    2015-12-01

    Full Text Available Hermeneutic Analysis of Cultural Context is a study of the process of interpretation of media texts, cultural and historical factors influencing the viewpoint agencies / media text and the author's point of view on the audience. Hermeneutical analysis suggests a media text comprehension through comparison with cultural tradition and reality; penetration into the logic of media texts; analysis skills through comparison of artistic images in the historical and cultural context. Thus, the system is subject to analysis media and its operation in society, human interaction, language, and media using. For the purposes of media education in this case stand out: the creation of a culture of interaction with the media, the development of the perception of different types of information, skills analysis and interpretation of media texts, the formation of critical thinking, creativity in the field of media. This article presented the maindirections for Hermeneutic Analysis of Cultural Context on media literacy education classes for student audience, including the examples of creative problems and issues associated with this type of the analysis in the context of media literacy education problems, ie based on six key concepts of media literacy education: agency, category, language, technology, audience, representation. The author supposes that the Hermeneutic Analysis of Cultural Context of media texts on media literacy education classes can significantly develop media competence of students, including critical thinking.

  3. 78 FR 49764 - Certain Electronic Digital Media Devices and Components Thereof; Commission's Final Determination...

    Science.gov (United States)

    2013-08-15

    ... COMMISSION Certain Electronic Digital Media Devices and Components Thereof; Commission's Final Determination...'') (collectively, ``Samsung''), from importing certain electronic digital media devices that infringe one or more... digital media devices and components thereof by reason of infringement of certain claims of the '949,...

  4. Culture Media and Individual Hosts Affect the Recovery of Culturable Bacterial Diversity from Amphibian Skin.

    Science.gov (United States)

    Medina, Daniel; Walke, Jenifer B; Gajewski, Zachary; Becker, Matthew H; Swartwout, Meredith C; Belden, Lisa K

    2017-01-01

    One current challenge in microbial ecology is elucidating the functional roles of the large diversity of free-living and host-associated bacteria identified by culture-independent molecular methods. Importantly, the characterization of this immense bacterial diversity will likely require merging data from culture-independent approaches with work on bacterial isolates in culture. Amphibian skin bacterial communities have become a recent focus of work in host-associated microbial systems due to the potential role of these skin bacteria in host defense against the pathogenic fungus Batrachochytrium dendrobatidis (Bd), which is associated with global amphibian population declines and extinctions. As there is evidence that some skin bacteria may inhibit growth of Bd and prevent infection in some cases, there is interest in using these bacteria as probiotic therapy for conservation of at-risk amphibians. In this study, we used skin swabs from American toads (Anaxyrus americanus) to: (1) assess the diversity and community structure of culturable amphibian skin bacteria grown on high and low nutrient culture media, (2) determine which culture media recover the highest proportion of the total skin bacterial community of individual toads relative to culture-independent data, and (3) assess whether the plated communities from the distinct media types vary in their ability to inhibit Bd growth in in-vitro assays. Overall, we found that culture media with low nutrient concentrations facilitated the growth of more diverse bacterial taxa and grew distinct communities relative to media with higher nutrient concentrations. Use of low nutrient media also resulted in culturing proportionally more of the bacterial diversity on individual toads relative to the overall community defined using culture-independent methods. However, while there were differences in diversity among media types, the variation among individual hosts was greater than variation among media types, suggesting that

  5. Designing media for animal cell culture: CHO cells, the industrial standard.

    Science.gov (United States)

    Landauer, Karlheinz

    2014-01-01

    The success of culturing CHO cells solely depends on functionality of the used media. Cell culture technology is more than 50 years old, and the knowledge of cell requirements increased steadily. In the beginning, animal-sourced components were the key to growth. Nowadays state-of-the-art media do not contain any animal or naturally sourced components. The compositions are based on scientific awareness of the needs of the cells. The result is high lot-to-lot consistency and high performance.In this book section, a method for the development of a synthetic, animal component-free medium is described. The composition is based on public available formulations and information based on the work of many scientists printed in numerous papers and manuscripts. The method shall help beginners to design their own medium, although some knowledge of biochemistry and animal cells is still required.

  6. Mass Media and Cultural Memory: Idealization of Values

    Directory of Open Access Journals (Sweden)

    Liljana Siljanovska

    2014-12-01

    Full Text Available The theoretical approach in defining the means for mass communication expressed in functionalist theory, especially in John Riley’s model, determines mass media as a social subsystem which is functionally connected with other systems in society that arises from their mutual conditionality and their causative and consequential connection with politics, economy, education, socialization and culture. The functions of articulating opinion by themselves problematize the creation of creative-thinking public because the imposition of topics, representation of individuals, values and norms of a culture, a space, a time is mediated by the ideological and functional mechanism of an organized structuring and transfer of messages simultaneously to as big an audience as possible. The vastness of the audience simply cannot by itself be understood as democratization of the culture in its broadest sense or simply because it is not a high, elite culture intended solely for a certain number of users.  It is that exact media reality, which almost always and exclusively is created through the selection of facts and values in relation to the audience and the factor of time, which simultaneously problematizes individual and collective memory. In the era of postmodernism and globalization of societies, media shaped content, in different mass media, especially on TV and the Internet, stimulate cultural development and pluralism of ideas in intercultural communication. However at the same time the setting of the stage for a media product, imposed by market logic of supply and demand erases the borders of difference, restructures the modalities of cultural identifiers and relativizes the dimensions of cultural identity through the unification of values transformed in surpassed or modern collective memories and concepts, such as – Balkanization, Americanization, Europeanization, civil society.

  7. Genotoxic Effects of Culture Media on Human Pluripotent Stem Cells

    Science.gov (United States)

    Prakash Bangalore, Megha; Adhikarla, Syama; Mukherjee, Odity; Panicker, Mitradas M.

    2017-01-01

    Culture conditions play an important role in regulating the genomic integrity of Human Pluripotent Stem Cells (HPSCs). We report that HPSCs cultured in Essential 8 (E8) and mTeSR, two widely used media for feeder-free culturing of HPSCs, had many fold higher levels of ROS and higher mitochondrial potential than cells cultured in Knockout Serum Replacement containing media (KSR). HPSCs also exhibited increased levels of 8-hydroxyguanosine, phospho-histone-H2a.X and p53, as well as increased sensitivity to γ-irradiation in these two media. HPSCs in E8 and mTeSR had increased incidence of changes in their DNA sequence, indicating genotoxic stress, in addition to changes in nucleolar morphology and number. Addition of antioxidants to E8 and mTeSR provided only partial rescue. Our results suggest that it is essential to determine cellular ROS levels in addition to currently used criteria i.e. pluripotency markers, differentiation into all three germ layers and normal karyotype through multiple passages, in designing culture media. PMID:28176872

  8. Proctection of Culture in Global Media Age

    OpenAIRE

    Judhariksawan

    2016-01-01

    Indonesia is the largest tropical archipelago in the South-East Asian region which consists of over 17,500 islands. It is located among two oceans and two continents with population over 246 million people, making it the fifth most populous nation in the world. Indonesia is a portrait of a country that reflects the diversity of culture. There are more than 300 ethnic groups, among others, Acehnese, Bataknese, Minangkabau, Betawi, Baduy, Javanese, Madurese, Balinese, Dayak, Banjar, Minahasans,...

  9. The Cultural Component of Language Teaching.

    Science.gov (United States)

    Kramsch, Claire

    1995-01-01

    This article proposes a theoretical framework for teaching culture through language that suspends the traditional dichotomy between the universal and the particular in language teaching and embraces the particular as a platform for dialogue and as a common struggle to realign differences. (30 references) (Author/CK)

  10. German financial media's responsiveness to Deutsche Bank's cultural change

    NARCIS (Netherlands)

    Strauß, N.

    2015-01-01

    Based on first-order and second-order agenda building theory, this study analyzes the responsiveness of German financial media to frames of the "cultural change" proclaimed in the banking industry, exemplified by Deutsche Bank. Findings suggest a difference between the two major German financial med

  11. Components of cultural competence in three mental health programs.

    Science.gov (United States)

    Siegel, Carole; Haugland, Gary; Reid-Rose, Lenora; Hopper, Kim

    2011-06-01

    The aim of this study was to identify components of cultural competence in mental health programs developed for cultural groups by community and mental health professionals from these groups. Three programs were studied: a prevention program primarily serving African-American and Afro-Caribbean youth, a Latino adult acute inpatient unit, and a Chinese day treatment program in a community-based agency. Nine study-trained field researchers used a semistructured instrument that captures program genealogy, structure, processes, and cultural infusion. Program cultural elements were identified from field notes and from individual and group interviews of consumers and staff (N=104). A research-group consensus process with feedback from program staff was used to group elements by shared characteristics into the program components of cultural competence. Components included communication competencies (with use of colloquialisms and accepted forms of address); staff in culturally acceptable roles; culturally framed trust building (such as pairing youths with mentors), stigma reduction, friendly milieus (such as serving culturally familiar foods and playing music popular with the culture), and services; and peer, family, and community involvement (including use of peer counselors and mentors, hosting parent weekends, and linking clients with senior center and community services). Incorporating these components into any program in which underserved cultural populations are seen is recommended for improving cultural competence.

  12. Culture media used in the micropropagation of orchids hybrids

    Directory of Open Access Journals (Sweden)

    Luzia Yuriko Miyata

    2014-09-01

    Full Text Available In orchid cultivation, tissue culture has been an important and effective tool for obtaining good quality seedlings on a large scale and within a short period of time. This study aims to evaluate culture media on the in vitro growth and development of orchid hybrids. Seedlings 1-1.5 cm long, deriving from in vitro germination of seeds, were inoculated in 250 mL flasks containing 50 mL of culture medium, with salts from the media where each of them were treated. Treatments consisted of applying culture media (MS; DSD1; Knudson; B5; and WPM, combined with 5 orchid hybrids (CW1, CW2, CW3, CI e BLC. The media were added 2 g L-1 of activated charcoal and solidified with 5 g L-1 of agar, and their pH was adjusted to 5.8 ± 0.1, before autoclaving at 121°C and at 1.1 atm for 20 minutes. After sterilization of media, seedlings were inoculated in a laminar flow chamber and then kept in a growth room at the temperature of 25 ± 1ºC, with photoperiod of 16 h and irradiance of 32 ?mol.m–2.s–1. The experimental design was completely randomized, in a factorial 5 x 5 scheme, containing 6 seedlings per repetition. After 120 days, we evaluated root number, shoot number, leaf and root length, and fresh root weight. In in vitro cultivation of orchids, the media standing out are MS and DSD1 and the hybrids CI, CW1, and BLC.

  13. [Isolation of an Paracoccidioides brasiliensis exoantigen from solid culture media].

    Science.gov (United States)

    Gago, J; Godio, C; Ochoa, L; Negroni, R; Nejamkis, M R

    1995-01-01

    The goal of this work was to develop in solid medium a fast method to obtain Paracoccidioides brasiliensis (Pb) with a high yield. Four culture media were assayed: Sabouraud honey-agar, Sabouraud dextrose-agar, tomato -agar-medium (TOM) and a medium based on grape pulp. The most exhuberant growth was observed in medium based on grape pulp. Antigen was prepared in microscale at 6, 10 and 15 days incubation of solid cultures and the crude product concentrated by means of Centriplus tubes (Helena, France). Isolated antigens were subjected to polyacrylamide gel electrophoresis, followed by immunolabelling and detection of the characteristic gp45 antigen employing human and Pb-infected rat sera. Best results were observed after 10 days culture in grape medium. None of the other three media afforded comparable results.

  14. Characterization of the volatile oil compositions from Hypericum perforatum L. shoot cultures in different basal media

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Morshedloo

    2017-02-01

    Full Text Available St. John’s wort (Hypericum perforatum L. is the most important species of the genus Hypericum and produces a wide range of chemical constituents including essential oil. Regarding advantages of in vitro culture techniques in production of desired metabolites, the present study was aimed to investigate volatile constituents of H. perforatum shoots cultured in different basal media. Shoot cultures were established by culturing six nodes of aseptic plants in three liquid media including MS (Murashige and Skoog, B5 (Gamborg B-5 and half-strength B5 containing 30 g L-1 sucrose and 0.5 mg L-1 BA (6-benzyladenine. According to the results, growth and profile of volatile constituents of cultured shoots were affected by the type of medium used and shoots cultured in the B5 medium exhibited the highest growth which was reached to 42.95 g flask-1. On the other hand, 44 components were totally identified by GC-FID and GC-MS analysis of essential oils of cultured shoots. Decane (27.7%, menthol (8.9%, methyl decanoate (4.6% and β-elemene (4.6% were the major volatile constituents of the shoots cultured in MS medium, while eudesma4(15,7-dien-1-β-ol (8.1-7.5%, thymol (7-7.2% and 1,4-trans-1,7-trans-acorenone (5.2-5.5% were found as the principal components of shoots cultured in B5 and half-strength B5 media.

  15. How do culture media influence in vitro perivascular cell behavior?

    Science.gov (United States)

    Huber, Birgit; Volz, Ann-Cathrin; Kluger, Petra Juliane

    2015-12-01

    Perivascular cells are multilineage cells located around the vessel wall and important for wall stabilization. In this study, we evaluated a stem cell media and a perivascular cell-specific media for the culture of primary perivascular cells regarding their cell morphology, doubling time, stem cell properties, and expression of cell type-specific markers. When the two cell culture media were compared to each other, perivascular cells cultured in the stem cell medium had a more elongated morphology and a faster doubling rate and cells cultured in the pericyte medium had a more typical morphology, with several filopodia, and a slower doubling rate. To evaluate stem cell properties, perivascular cells, CD146(-) cells, and mesenchymal stem cells (MSCs) were differentiated into the adipogenic, osteogenic, and chondrogenic lineages. It was seen that perivascular cells, as well as CD146(-) cells and MSCs, cultured in stem cell medium showed greater differentiation than cells cultured in pericyte-specific medium. The expression of pericyte-specific markers CD146, neural/glial antigen 2 (NG2), platelet-derived growth factor receptor-β (PDGFR-β), myosin, and α-smooth muscle actin (α-SMA) could be found in both pericyte cultures, as well as to varying amounts in CD146(-) cells, MSCs, and endothelial cells. The here presented work shows that perivascular cells can adapt to their in vitro environment and cell culture conditions influence cell functionality, such as doubling rate or differentiation behavior. Pericyte-specific markers were shown to be expressed also from cells other than perivascular cells. We can further conclude that CD146(+) perivascular cells are inhomogeneous cell population probably containing stem cell subpopulations, which are located perivascular around capillaries.

  16. Man & the Media II: Media and Cross-Cultural Communication in Foreign Language Learning.

    Science.gov (United States)

    Kelling, Hans-Wilhelm; Niedzielski, Henry

    1987-01-01

    Summarizes presentations given at the second annual symposium "Man and the Media" at the Institute for Romance Languages at the University of Saarbrucken, West Germany in September 1986. Includes comparisons between French and German news coverage on TV, teaching French using TV, and cross-cultural problems in using instructional videos.…

  17. Man & the Media II: Media and Cross-Cultural Communication in Foreign Language Learning.

    Science.gov (United States)

    Kelling, Hans-Wilhelm; Niedzielski, Henry

    1987-01-01

    Summarizes presentations given at the second annual symposium "Man and the Media" at the Institute for Romance Languages at the University of Saarbrucken, West Germany in September 1986. Includes comparisons between French and German news coverage on TV, teaching French using TV, and cross-cultural problems in using instructional videos.…

  18. American Multinational Media and the Expansion of American Mass Culture

    Institute of Scientific and Technical Information of China (English)

    王茜

    2011-01-01

    With the development of globalization and gradual intensification of mutual dependence of the world,"hard power" which indudes economic and military strength is no longer the primary consideration for many countries.They tend to pay more attention to the attractiveness of their "soft power",which includes values,life style,ideology and so forth.Just as Joseph S.Ny,the famous professor of Harvard who puts forward the concept of soft power,says,"only by widespread communication and diffusion can a country reinforces its soft power".This is mass media's function.Since the 1990s,tens of thousands cultural products brought in from America are exported to foreign countries,including popular music,films,TV programs,magazines,books and so forth.By making advantage of its monopoly position in media circle,the United States pursues cultural hegemonism and expands its popttlar culture all over the world.This thesis takes a general view of the great advantages of America multi-national media groups and how they push forward the American popular culture all over the world.

  19. 21 CFR 864.2360 - Mycoplasma detection media and components.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Cell And Tissue Culture Products... microbial contaminant in cell cultures. (b) Classification. Class I (general controls). These devices...

  20. Introduction Social Media as a Component of Reading Courses

    Science.gov (United States)

    Bresmer, Allison; Hynes-Musnisky, Elizabeth; Newlin-Wagner, Susan; Sikinger-Golde, Kerry

    2015-01-01

    Incorporating various forms of social media in the classroom enhances learning and motivates students to contribute to class lessons and activities. This article discusses some successful approaches for implementing wikis, Facebook, Pinterest and Goodreads in the classroom. Social media in the classroom has proved beneficial to developmental and…

  1. Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy

    Directory of Open Access Journals (Sweden)

    Katarína Mlynáriková

    2015-11-01

    Full Text Available Raman spectroscopy has a broad range of applications across numerous scientific fields, including microbiology. Our work here monitors the influence of culture media on the Raman spectra of clinically important microorganisms (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans. Choosing an adequate medium may enhance the reproducibility of the method as well as simplifying the data processing and the evaluation. We tested four different media per organism depending on the nutritional requirements and clinical usage directly on a Petri dish. Some of the media have a significant influence on the microbial fingerprint (Roosvelt-Park Institute Medium, CHROMagar and should not be used for the acquisition of Raman spectra. It was found that the most suitable medium for microbiological experiments regarding these organisms was Mueller-Hinton agar.

  2. McLuhan and the Cultural Theory of Media

    Directory of Open Access Journals (Sweden)

    Mark Poster

    2010-02-01

    Full Text Available Media are surely central to Western societies of the past several centuries and to the emerging global societies of the contemporary era and the future. There is a thickening, an intensification and an increasing complexity to the use of information machines, technologies that are necessary in the production, reproduction, storing and distribution of texts, images and sounds, the constituent elements of culture. The phenomenon has been termed a “media ecology,” adding a new layer to the ecologies of animal, vegetable and mineral. It behooves anyone engaged in critical discourse to take serious account of media. I argue they offer a key to understanding the process of globalization in relation to a new configuration of interaction between humans and machines.

  3. Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy.

    Science.gov (United States)

    Mlynáriková, Katarína; Samek, Ota; Bernatová, Silvie; Růžička, Filip; Ježek, Jan; Hároniková, Andrea; Šiler, Martin; Zemánek, Pavel; Holá, Veronika

    2015-11-24

    Raman spectroscopy has a broad range of applications across numerous scientific fields, including microbiology. Our work here monitors the influence of culture media on the Raman spectra of clinically important microorganisms (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans). Choosing an adequate medium may enhance the reproducibility of the method as well as simplifying the data processing and the evaluation. We tested four different media per organism depending on the nutritional requirements and clinical usage directly on a Petri dish. Some of the media have a significant influence on the microbial fingerprint (Roosvelt-Park Institute Medium, CHROMagar) and should not be used for the acquisition of Raman spectra. It was found that the most suitable medium for microbiological experiments regarding these organisms was Mueller-Hinton agar.

  4. 21 CFR 866.2480 - Quality control kit for culture media.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Quality control kit for culture media. 866.2480... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2480 Quality control kit for culture media. (a) Identification. A quality control kit for culture media is a device...

  5. Rapid characterization and quality control of complex cell culture media solutions using raman spectroscopy and chemometrics.

    Science.gov (United States)

    Li, Boyan; Ryan, Paul W; Ray, Bryan H; Leister, Kirk J; Sirimuthu, Narayana M S; Ryder, Alan G

    2010-10-01

    The use of Raman spectroscopy coupled with chemometrics for the rapid identification, characterization, and quality assessment of complex cell culture media components used for industrial mammalian cell culture was investigated. Raman spectroscopy offers significant advantages for the analysis of complex, aqueous-based materials used in biotechnology because there is no need for sample preparation and water is a weak Raman scatterer. We demonstrate the efficacy of the method for the routine analysis of dilute aqueous solution of five different chemically defined (CD) commercial media components used in a Chinese Hamster Ovary (CHO) cell manufacturing process for recombinant proteins.The chemometric processing of the Raman spectral data is the key factor in developing robust methods. Here, we discuss the optimum methods for eliminating baseline drift, background fluctuations, and other instrumentation artifacts to generate reproducible spectral data. Principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA) were then employed in the development of a robust routine for both identification and quality evaluation of the five different media components. These methods have the potential to be extremely useful in an industrial context for "in-house" sample handling, tracking, and quality control.

  6. Stability of resazurin in buffers and mammalian cell culture media

    DEFF Research Database (Denmark)

    Rasmussen, Eva; Nicolaisen, G.M.

    1999-01-01

    The utility of a ferricyanide/ferrocyanide system used in the AlamarBlue(TM) (Serotec, Oxford, UK) vital. dye to inhibit the reduction of resazurin by mammalian cell culture media is questioned. Resazurin was found to be relatively stable when dissolved in phosphate-buffered saline (PBS). The use...... of HEPES resulted in a huge immediate dye reduction, which was significantly enhanced by exposure to diffuse light from fluorescent tubes in the laboratory 8 h per day. The reduction of resazurin by various cell culture media was time and temperature dependent, and it was significantly enhanced...... by prolonged exposure to the Light in the laboratory. A pronounced reduction of resazurin was observed in the RPMI 1640 medium, which contains 1 mg/L of reduced glutathione. No significant differences in resazurin reduction were observed between McCoy's 5A medium, Dulbecco's Modified Essential medium, and Ham...

  7. The replacement of serum by hormones in cell culture media.

    Science.gov (United States)

    Sato, G; Hayashi, I

    1976-12-01

    The replacement of serum by hormones in cell culture media. (Reemplazo del suero por hormonas en el medio de cultivo de células). Arch. Biol. Med. Exper. 10: 120-121, 1976. The serum used in cell culture media can be replaced by a mixture of hormones and some accesory blood factors. The pituitary cell line GH3 can be grown in a medium in which serum is replaced by triiodothyronine, transferrin, parathormone, tyrotrophin releasing hormone and somatomedins. Hela and BHK cell strains can also be grown in serum free medium supplemented with hormones. Each cell type appears to have different hormonal requirements yet it may found that some hormones are required for most cell types.

  8. Culture of green algae Chlorella ellipsoidea in inexpensive media

    OpenAIRE

    Rahman,M. S.; Hossain, M Anwar; Fatema, S.; Hossain, M. Amzad

    2005-01-01

    The culture of the of green alga Chlorella ellipsoidea was conducted under natural conditions at the same place simultaneously in five different media, viz., medium-I (inorganic medium), medium-II (powdered whole-pulse medium), medium-III (medium of pulse bran), medium-IV (mixed medium = 50% inorganic medium + 50% whole-pulse powder medium), medium-V (mixed medium = 50% inorganic medium + 50% pulse bran medium). The culture was done in 500 ml conical flask. Growth rates of C. ellipsoidea in f...

  9. Studying Mobile Media Cultural Technologies, Mobile Communication, and the IPhone

    CERN Document Server

    Hjorth, Larissa; Richardson, Ingrid

    2012-01-01

    The iPhone represents an important moment in both the short history of mobile media and the long history of cultural technologies. Like the Walkman of the 1980s, it marks a juncture in which notions about identity, individualism, lifestyle and sociality require rearticulation. this book explores not only the iPhone's particular characteristics, uses and "affects," but also how the "iPhone moment" functions as a barometer for broader patterns of change. In the iPhone moment, this study considers the convergent trajectories in the evolution of digital and mobile culture, and

  10. A mediação cultural na biblioteca escolar

    Directory of Open Access Journals (Sweden)

    Diego Andrés Salcedo

    2015-02-01

    Full Text Available Inúmeras são as bibliotecas escolares e muitos são os jovens estudantes que precisam frequentá-la para o auxílio em suas atividades ou para simplesmente ter um local silencioso para a concentração. Mas a biblioteca escolar pode ter muito mais a oferecer. O bibliotecário pode trabalhar como mediador e levar àquele ambiente, diversas atividades para o aperfeiçoamento do processo de aprendizagem cultural daquele público. A mediação cultural pode ser feita sob diversas formas, levando peças, pinturas, exposições das mais variadas formas a este local, tornando-se assim, um dispositivo cultural. E tão importante para o funcionamento deste dispositivo, é a interação das equipes ligadas à mediação. Um espaço físico adequado e mediadores conectados com o mundo atual são alguns dos fatores para a elaboração de uma boa mediação. A biblioteca escolar é analisada como um meio em que a cultura (seja ela qual for é encaminhada para esse público tão curioso, que possui a mente aberta para entrada de conhecimentos diversos, e consequentemente, indivíduos culturalmente ricos serão criados.

  11. Reassessing culture media and critical metabolites that affect adenovirus production.

    Science.gov (United States)

    Shen, Chun Fang; Voyer, Robert; Tom, Roseanne; Kamen, Amine

    2010-01-01

    Adenovirus production is currently operated at low cell density because infection at high cell densities still results in reduced cell-specific productivity. To better understand nutrient limitation and inhibitory metabolites causing the reduction of specific yields at high cell densities, adenovirus production in HEK 293 cultures using NSFM 13 and CD 293 media were evaluated. For cultures using NSFM 13 medium, the cell-specific productivity decreased from 3,400 to 150 vp/cell (or 96% reduction) when the cell density at infection was increased from 1 to 3 x 10(6) cells/mL. In comparison, only 50% of reduction in the cell-specific productivity was observed under the same conditions for cultures using CD 293 medium. The effect of medium osmolality was found critical on viral production. Media were adjusted to an optimal osmolality of 290 mOsm/kg to facilitate comparison. Amino acids were not critical limiting factors. Potential limiting nutrients including vitamins, energy metabolites, bases and nucleotides, or inhibitory metabolites (lactate and ammonia) were supplemented to infected cultures to further investigate their effect on the adenovirus production. Accumulation of lactate and ammonia in a culture infected at 3 x 10(6) cells/mL contributed to about 20% reduction of the adenovirus production yield, whereas nutrient limitation appeared primarily responsible for the decline in the viral production when NSFM 13 medium was used. Overall, the results indicate that multiple factors contribute to limiting the specific production yield at cell densities beyond 1 x 10(6) cells/mL and underline the need to further investigate and develop media for better adenoviral vector productions.

  12. Arginine and glutamine supplementation to culture media improves the performance of various channel catfish immune cells.

    Science.gov (United States)

    Pohlenz, Camilo; Buentello, Alejandro; Mwangi, Waithaka; Gatlin, Delbert M

    2012-05-01

    Specific components of both the innate and adaptive immune systems of channel catfish were evaluated after supplementation of culture media with arginine (ARG) and/or glutamine (GLN). Primary cell cultures of head-kidney macrophages (MØ) were used for phagocytic and bactericidal assays against Edwardsiella ictaluri. Additionally, proliferation assays were conducted with naïve peripheral blood lymphocytes (PBL) exposed to non-specific mitogens. To indirectly assess amino acid utilization of both MØ and PBL, amino acid levels, with emphasis on ARG and GLN, were evaluated in the basal medium before and after activation or mitogenic exposure. After bactericidal and proliferation assays, the sum of the media free amino acid pool significantly (P Glutamine levels in medium decreased by 38% and ARG by 18% during the bactericidal assay. Also, decreases of 52 and 46% from initial values were found after the proliferation assay for GLN and ARG, respectively. Macrophage phagocytosis and killing ability was significantly (P < 0.05) enhanced by ARG supplementation to culture media regardless of GLN supplementation. Proliferation of naïve T- and B-lymphocytes upon mitogenic exposure was significantly (P < 0.05) enhanced by supplementing ARG and GLN to the media, but limited synergistic effects were observed. These results suggest that in vitro, ARG and GLN are important substrates and immunomodulators of both innate and adaptive responses in fish leukocytes, and further highlights the potential use of ARG and GLN as immunonutrients in aquafeeds.

  13. Study on recycling of waste rubbers as medium components for hydroponic culture of rose

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jin-Kuk; Lee, Hyung-Gyu; Jeong, Byoung-Ryong; Hwang, Seung-Jae [Gyeongsang National Univ., Kumi(Korea)

    2000-06-30

    Recently, the efficient disposal of the waste rubber is necessary due to increasing amount of the waste rubbers. In this paper, method of recycling waste rubbers as components of medium for hydroponic rose culture was suggested. We investigated growth of rose, and macro- and micro-elements, pH and EC of the media amended with waste rubber. In the beginning of culture, stress symptoms such as thin brittle stem and incipient wilting were observed, but they disappeared in a few weeks. Concentration of Zn{sup 2+} in media at flowering increased in proportion to contents of waste tire in the media. pH of media at flowering were in the range of 5.70 to 6.35. Rose growth in all media, except in waste rock wool mixed with EPDM powder at 9:3 ratio, was normal and equivalent to the control in terms of stem length, number of stems harvested and fresh weight. (author). 10 refs., 5 tabs., 4 figs.

  14. Translating Cuba: Diasporic writing between moving cultures and moving media

    Directory of Open Access Journals (Sweden)

    Andrea Gremels

    2015-04-01

    Full Text Available This article discusses the interrelation between transculturality and transmediality with an emphasis on processes of translation. It focuses on two examples of transcultural and transmedial writing taken from contemporary Cuban literature in Paris: Miguel Sales's recontextualization of Cuban popular music in Paris and William Navarrete's ekphrastic reinscription of his island into the realm of French romantic painting. The case studies are significant in this context because they show how cultural borders are simultaneously set and transgressed at medial crossings—between music and poetry, text, and image. Thus, cultural translations go hand in hand with medial transpositions that include forms of rewriting, recomposition, and revisualization. The connection between moving cultures and moving media also points to the question of “travelling memory” in diaspora.

  15. Washington Crossing the Media: American Presidential Rhetoric and Cultural Iconography

    Directory of Open Access Journals (Sweden)

    Jutta Ernst

    2012-04-01

    Full Text Available The Revolutionary War has been of prime importance to American self-perceptions and to the formation of its national identity. As a foundational moment with a strong mythical dimension it has become a cherished point of reference for the nation’s leaders, who, in their speeches and written communications, rely on the most widely accepted cultural iconography to recall this event. A time-honored, traditional discourse might, however, go together with the use of contemporary media technology as a means of distribution, as in the case of Barack Obama. Framing Obama’s rhetorical strategies within 19th- and 20th-century artistic representations of one specific historical moment from the Revolutionary War, Washington’s crossing of the Delaware river, this paper seeks to contribute to an enlarged understanding of the intricate relations between politics, the arts, and media development and of the ways they appropriate the past

  16. Birthweight distribution in ART singletons resulting from embryo culture in two different culture media compared with the national population

    DEFF Research Database (Denmark)

    Lemmen, Josephine Gabriela; Pinborg, Anja; Rasmussen, S

    2014-01-01

    STUDY QUESTION: Is there a difference in birthweight distribution in ART singletons born after IVF culture in two different culture media? SUMMARY ANSWER: There is no effect of culture media on both crude and adjusted birthweight distributions in ART singletons from nulliparous mothers. WHAT...... IS KNOWN ALREADY: Studies on human ART singletons have reported a difference in birthweight in singletons following IVF culture in different culture media. However, other studies comparing different culture media have not shown any significant differences in birthweight. STUDY DESIGN, SIZE, DURATION...... culture media groups, we found the same slightly lower mean birthweight in IVF/ICSI singletons when compared with the national birth cohort as has been previously reported (Cook-d2 + Medicult-d2 + d3 versus birth cohort; girls: P

  17. Media Aid Beyond the Factual: Culture, Development, and Audiovisual Assistance

    Directory of Open Access Journals (Sweden)

    Benjamin A. J. Pearson

    2015-01-01

    Full Text Available This paper discusses audiovisual assistance, a form of development aid that focuses on the production and distribution of cultural and entertainment media such as fictional films and TV shows. While the first audiovisual assistance program dates back to UNESCO’s International Fund for the Promotion of Culture in the 1970s, the past two decades have seen a proliferation of audiovisual assistance that, I argue, is related to a growing concern for culture in post-2015 global development agendas. In this paper, I examine the aims and motivations behind the EU’s audiovisual assistance programs to countries in the Global South, using data from policy documents and semi-structured, in-depth interviews with Program Managers and administrative staff in Brussels. These programs prioritize forms of audiovisual content that are locally specific, yet globally tradable. Furthermore, I argue that they have an ambivalent relationship with traditional notions of international development, one that conceptualizes media not only as a means to achieve economic development and human rights aims, but as a form of development itself.

  18. Practicing Critical Media Literacy Education: Developing a Community of Inquiry among Teachers Using Popular Culture

    Science.gov (United States)

    Flores-Koulish, Stephanie

    2010-01-01

    Media literacy compels us to look anew at the most mundane, that which surrounds us: the media and our popular culture. From there media literacy compels us to accept that the media are constructed and to seek various ways to analyze them, while considering our own beliefs to evaluate for ourselves an ultimate interpretation. This process has the…

  19. Media Bound Culture of Women’s Identity and Desire

    Directory of Open Access Journals (Sweden)

    Adhikari Harasankar

    2016-12-01

    Full Text Available The post global era is signified in terms of women’s changed status in the patriarchal society of India. Their participation in the work force is not experienced as good or as desired because they have to face rampant violence related to their reorganized desires. The media-bound culture is a prime cause of their mimetic behavior in lifestyle and other choices. This paper attempts to raise two questions: Might this be the major cause of the violence? Is there a relationship between consumerism and women’s body as sex commodity?

  20. Modeling media as latent semantics based on cognitive components

    DEFF Research Database (Denmark)

    Petersen, Michael Kai

    dimensionality. While top-down, patterns of emotional categorization emerge by defining term vector distances to affective adjectives, that constrain the latent semantic structures according to the neurophysiological dimensions of valence and arousal. The thesis thus combines elements of machine learning...... with aspects of cognitive linguistics that potentially could be utilized in applications ranging from information retrieval and media personalization, to emotional brand building or neuroscientific modeling of syntax and semantics....

  1. Plant-based culture media: Efficiently support culturing rhizobacteria and correctly mirror their in-situ diversity

    Science.gov (United States)

    Youssef, Hanan H.; Hamza, Mervat A.; Fayez, Mohamed; Mourad, Elhussein F.; Saleh, Mohamed Y.; Sarhan, Mohamed S.; Suker, Ragab M.; Eltahlawy, Asmaa A.; Nemr, Rahma A.; El-Tahan, Mahmod; Ruppel, Silke; Hegazi, Nabil A.

    2015-01-01

    Our previous publications and the data presented here provide evidences on the ability of plant-based culture media to optimize the cultivability of rhizobacteria and to support their recovery from plant-soil environments. Compared to the tested chemically-synthetic culture media (e.g. nutrient agar and N-deficient combined-carbon sources media), slurry homogenates, crude saps, juices and powders of cactus (Opuntia ficus-indica) and succulent plants (Aloe vera and Aloe arborescens) were rich enough to support growth of rhizobacteria. Representative isolates of Enterobacter spp., Klebsiella spp., Bacillus spp. and Azospirillum spp. exhibited good growth on agar plates of such plant-based culture media. Cell growth and biomass production in liquid batch cultures were comparable to those reported with the synthetic culture media. In addition, the tested plant-based culture media efficiently recovered populations of rhizobacteria associated to plant roots. Culturable populations of >106–108 cfu g−1 were recovered from the ecto- and endo-rhizospheres of tested host plants. More than 100 endophytic culture-dependent isolates were secured and subjected to morphophysiological identification. Factor and cluster analyses indicated the unique community structure, on species, genera, class and phyla levels, of the culturable population recovered with plant-based culture media, being distinct from that obtained with the chemically-synthetic culture media. Proteobacteria were the dominant (78.8%) on plant-based agar culture medium compared to only 31% on nutrient agar, while Firmicutes prevailed on nutrient agar (69%) compared to the plant-based agar culture media (18.2%). Bacteroidetes, represented by Chryseobacterium indologenes, was only reported (3%) among the culturable rhizobacteria community of the plant-based agar culture medium. PMID:26966571

  2. Plant-based culture media: Efficiently support culturing rhizobacteria and correctly mirror their in-situ diversity.

    Science.gov (United States)

    Youssef, Hanan H; Hamza, Mervat A; Fayez, Mohamed; Mourad, Elhussein F; Saleh, Mohamed Y; Sarhan, Mohamed S; Suker, Ragab M; Eltahlawy, Asmaa A; Nemr, Rahma A; El-Tahan, Mahmod; Ruppel, Silke; Hegazi, Nabil A

    2016-03-01

    Our previous publications and the data presented here provide evidences on the ability of plant-based culture media to optimize the cultivability of rhizobacteria and to support their recovery from plant-soil environments. Compared to the tested chemically-synthetic culture media (e.g. nutrient agar and N-deficient combined-carbon sources media), slurry homogenates, crude saps, juices and powders of cactus (Opuntia ficus-indica) and succulent plants (Aloe vera and Aloe arborescens) were rich enough to support growth of rhizobacteria. Representative isolates of Enterobacter spp., Klebsiella spp., Bacillus spp. and Azospirillum spp. exhibited good growth on agar plates of such plant-based culture media. Cell growth and biomass production in liquid batch cultures were comparable to those reported with the synthetic culture media. In addition, the tested plant-based culture media efficiently recovered populations of rhizobacteria associated to plant roots. Culturable populations of >10(6)-10(8) cfu g(-1) were recovered from the ecto- and endo-rhizospheres of tested host plants. More than 100 endophytic culture-dependent isolates were secured and subjected to morphophysiological identification. Factor and cluster analyses indicated the unique community structure, on species, genera, class and phyla levels, of the culturable population recovered with plant-based culture media, being distinct from that obtained with the chemically-synthetic culture media. Proteobacteria were the dominant (78.8%) on plant-based agar culture medium compared to only 31% on nutrient agar, while Firmicutes prevailed on nutrient agar (69%) compared to the plant-based agar culture media (18.2%). Bacteroidetes, represented by Chryseobacterium indologenes, was only reported (3%) among the culturable rhizobacteria community of the plant-based agar culture medium.

  3. 78 FR 33110 - Certain Electronic Digital Media Devices and Components Thereof; Determination To Review a Remand...

    Science.gov (United States)

    2013-06-03

    ... COMMISSION Certain Electronic Digital Media Devices and Components Thereof; Determination To Review a Remand... the sale within the United States after importation of certain electronic digital media devices and..., including in the JP published unexamined application HII-288766 (``Kawano'') and the YP-T7J portable...

  4. Improved Murine Blastocyst Quality and Development in a Single Culture Medium Compared to Sequential Culture Media.

    Science.gov (United States)

    Hennings, Justin M; Zimmer, Randall L; Nabli, Henda; Davis, J Wade; Sutovsky, Peter; Sutovsky, Miriam; Sharpe-Timms, Kathy L

    2016-03-01

    Validate single versus sequential culture media for murine embryo development. Prospective laboratory experiment. Assisted Reproduction Laboratory. Murine embryos. Thawed murine zygotes cultured for 3 or 5 days (d3 or d5) in single or sequential embryo culture media developed for human in vitro fertilization. On d3, zygotes developing to the 8 cell (8C) stage or greater were quantified using 4',6-diamidino-2-phenylindole (DAPI), and quality was assessed by morphological analysis. On d5, the number of embryos reaching the blastocyst stage was counted. DAPI was used to quantify total nuclei and inner cell mass nuclei. Localization of ubiquitin C-terminal hydrolase L1 (UCHL1) and ubiquitin C-terminal hydrolase L3 (UCHL3) was reference points for evaluating cell quality. Comparing outcomes in single versus to sequential media, the odds of embryos developing to the 8C stage on d3 were 2.34 time greater (P = .06). On d5, more embryos reached the blastocyst stage (P = quality hatching blastocysts. Localization of UCHL1 and UCHL3 was diffuse and inappropriately dispersed throughout the cytoplasm in low-quality nonhatching blastocysts. Single medium yields greater cell numbers, an increased growth rate, and more hatching of murine embryos. Cytoplasmic UCHL1 and nuclear UHCL3 localization patterns were indicative of embryo quality. Our conclusions are limited to murine embryos but one might speculate that single medium may also be more beneficial for human embryo culture. Human embryo studies are needed. © The Author(s) 2015.

  5. Plant-based culture media: Efficiently support culturing rhizobacteria and correctly mirror their in-situ diversity

    OpenAIRE

    Youssef, Hanan H.; Hamza, Mervat A.; Fayez, Mohamed; Mourad, Elhussein F.; Saleh, Mohamed Y.; Sarhan, Mohamed S.; Suker, Ragab M.; Eltahlawy, Asmaa A.; Nemr, Rahma A.; El-Tahan, Mahmod; Ruppel, Silke; Hegazi, Nabil A.

    2015-01-01

    Our previous publications and the data presented here provide evidences on the ability of plant-based culture media to optimize the cultivability of rhizobacteria and to support their recovery from plant-soil environments. Compared to the tested chemically-synthetic culture media (e.g. nutrient agar and N-deficient combined-carbon sources media), slurry homogenates, crude saps, juices and powders of cactus (Opuntia ficus-indica) and succulent plants (Aloe vera and Aloe arborescens) were rich ...

  6. Cultures in orbit: Satellite technologies, global media and local practice

    Science.gov (United States)

    Parks, Lisa Ann

    Since the launch of Sputnik in 1957, satellite technologies have had a profound impact upon cultures around the world. "Cultures in Orbit" examines these seemingly disembodied, distant relay machines in relation to situated social and cultural processes on earth. Drawing upon a range of materials including NASA and UNESCO documents, international satellite television broadcasts, satellite 'development' projects, documentary and science fiction films, remote sensing images, broadcast news footage, World Wide Web sites, and popular press articles I delineate and analyze a series of satellite mediascapes. "Cultures in Orbit" analyzes uses of satellites for live television relay, surveillance, archaeology and astronomy. The project examines such satellite media as the first live global satellite television program Our World, Elvis' Aloha from Hawaii concert, Aboriginal Australian satellite programs, and Star TV's Asian music videos. In addition, the project explores reconnaissance images of mass graves in Bosnia, archaeological satellite maps of Cleopatra's underwater palace in Egypt, and Hubble Space Telescope images. These case studies are linked by a theoretical discussion of the satellite's involvement in shifting definitions of time, space, vision, knowledge and history. The satellite fosters an aesthetic of global realism predicated on instantaneous transnational connections. It reorders linear chronologies by revealing traces of the ancient past on the earth's surface and by searching in deep space for the "edge of time." On earth, the satellite is used to modernize and develop "primitive" societies. Satellites have produced new electronic spaces of international exchange, but they also generate strategic maps that advance Western political and cultural hegemony. By technologizing human vision, the satellite also extends the epistemologies of the visible, the historical and the real. It allows us to see artifacts and activities on earth from new vantage points

  7. Laboratory experience with radiometric detection of bacteremia with three culture media

    Energy Technology Data Exchange (ETDEWEB)

    Wicher, K.; Koscinski, D.

    1984-10-01

    In two long-term studies, the BACTEC radiometric system for detection of bacteremia was evaluated with three culture media each: (i) BACTEC media 6A (for aerobes) and 7B (for anaerobes) plus a thioglycolate medium and (ii) BACTEC media 6A, 7B, and 8A (hypertonic). In study 1, clinically significant isolates were identified in 1,873 (13.9%) of 13,432 blood cultures with all three media. The thioglycolate medium revealed 143 (1.1%) organisms not recovered from the 6A and 7B media. In study 2, isolates were identified in 1,135 (12.9%) of 8,759 cultures with all three media; 104 (1.2%) organisms were isolated only from the hypertonic medium. The increased yield of positive cultures in the three-medium system is likely due to the larger volume of blood cultured.

  8. Original people – Mapuche - Cultural identity - Social media - Digital divide

    Directory of Open Access Journals (Sweden)

    López-Vicent, Patricia

    2014-01-01

    Full Text Available This paper reflects on the value of the implementation of ICT in indigenous communities in southern Chile, related to the appreciation of cultural identity. Assuming the presence of ICT in all indigenous communities in the world, and specially in the Mapuche communities, we present a training-oriented approach from the concept of digital literacy, and introduce social media as tools available to any member of these communities, in order to access, create and disseminate information, and to communicate and collaborate with their community and other communities, geographically close or distant. The results presented in this article draw from an international cooperation project that started in 2010 between the University of La Frontera (Temuco, Chile and the University of Murcia (Murcia, Spain. This article in written in Spanish

  9. Establishing axenic cultures from mature pecan embryo explants on media with low water availability.

    Science.gov (United States)

    Obeidy, A A; Smith, M A

    1990-12-01

    Endophytic fungi associated with mature pecan (Carya illinoensis (Wangenh.) C. Koch) nuts prevented successful, contaminant-free in vitro culture of embryo expiants, even after rigorous surface disinfestation of the nuts and careful aseptic shelling. Disinfestation with sodium hypochlorite after shell removal was also unsuccessful, because even dilute concentrations which were ineffective against the fungal contaminants prevented subsequent growth from the embryo. Explanting media with low water availability which would not sustain growth of fungal contaminants, but supported growth from mature pecan embryos, were developed as an alternative disinfestation method. The explanting media were supplemented with 0.9-1.5% agar, and other media components were selectively omitted to test their influence on water availability and fungal growth. Disinfestation of up to 65% of the cultures was accomplished, depending on the medium formulation, compared to 100% loss to contamination on control medium (0.5% agar). A complete medium (containing sucrose, salts, vitamins, 18 μM BAP, and 5 μM IBA) with 1.5% agar provided control of contamination, and encouraged subsequent regeneration from the embryo expiants, which remained free of contaminant growth through subsequent subcultures.

  10. Popular Culture and Critical Media Literacy in Adult Education: Theory and Practice

    Science.gov (United States)

    Tisdell, Elizabeth J.

    2007-01-01

    This chapter introduces the volume, provides an overview of the theory and literature on popular culture and critical media literacy in education, and discusses ways to use popular culture in adult education.

  11. Culture in the Media: the Representation of Culture in "Jutarnji List"

    Directory of Open Access Journals (Sweden)

    Marko Pavlovski

    2015-09-01

    Full Text Available In today's picture of the widely circulated print media a famous assertion is that culture as a subject is decreasing in relevancy, as seen by the amount of pages dedicated to the cultural section and informations on cultural events, especially those of international importance, in the entire content of the most widely read newspapers. The aim of this paper is to show the presence of cultural themes in one of the most popular daily newspaper published in the Republic of Croatia, Jutarnji list. The research will cover the content of this daily for a period of one year (January 1st 2013 to 1st January 2014, to show the presence of cultural themes in it. I suppose, because of a prior knowledge of the materials, that notifications on events in culture at home and abroad will reside outside pages dedicated to the cultural section, so it will be necessary to examine the contents of whole numbers. Furthermore, I will compare the representation of various artistic genres, the presence of film art compared to the amount of articles devoted to literature, and I will devote particular attention to the content dedicated to the non-commercial aspects of a particular genre, for example the amount of information about events in the poetry scene, promotions or group exhibitions of young filmmakers at home and abroad.

  12. Use of pruning waste compost as a component in soilless growing media.

    Science.gov (United States)

    Benito, Marta; Masaguer, Alberto; De Antonio, Roberto; Moliner, Ana

    2005-03-01

    The objective of this work was to study the use of pruning wastes compost (PWC) as a growing media component for ornamental plants. The main physical, chemical and biological characteristics of PWC were analysed in order to evaluate its suitability for use in soil-less cultivation. Six growth substrates were prepared by mixing PWC with peat (P), ground leaves (GL), sand (S) and spent mushroom compost (SMC) in different proportions. Two different pot experiments were carried out to test its characteristics of production using perennial ryegrass (Lolium perenne L.) and cypress (Cupressus sempervirens L.) as indicators and the different media as treatments. The growth experiments showed that PWC required mixing with a nutrient-richer material to produce higher results. Therefore, substrates containing SMC (PWC+P+SMC and PWC+SMC) seems to be the most adequate growing media. After the statistical analysis, we concluded that the PWC could be used as a growing media component.

  13. Digital Media Literacy in a Sports, Popular Culture and Literature Course

    Science.gov (United States)

    Fortuna, Carolyn

    2015-01-01

    This article considers how media sports culture is an apt space for digital media literacy instruction. Describing a senior year high school English course that requires students to deconstruct and compose with sports media texts, the author outlines how learning modules, analysis of curated collections of texts through heuristics, and mentor…

  14. Digital Media Literacy in a Sports, Popular Culture and Literature Course

    Science.gov (United States)

    Fortuna, Carolyn

    2015-01-01

    This article considers how media sports culture is an apt space for digital media literacy instruction. Describing a senior year high school English course that requires students to deconstruct and compose with sports media texts, the author outlines how learning modules, analysis of curated collections of texts through heuristics, and mentor…

  15. Comparison of Fluorescence Microscopy and Different Growth Media Culture Methods for Acanthamoeba Keratitis Diagnosis.

    Science.gov (United States)

    Peretz, Avi; Geffen, Yuval; Socea, Soergiu D; Pastukh, Nina; Graffi, Shmuel

    2015-08-01

    Acanthamoeba keratitis (AK), a potentially blinding infection of the cornea, is caused by a free-living protozoan. Culture and microscopic examination of corneal scraping tissue material is the conventional method for identifying Acanthamoeba. In this article, we compared several methods for AK diagnosis of 32 patients: microscopic examination using fluorescent dye, specific culture on growth media-non-nutrient agar (NNA), culture on liquid growth media-peptone yeast glucose (PYG), and TYI-S-33. AK was found in 14 patients. Thirteen of the specimens were found AK positive by fluorescence microscopic examination, 11 specimens were found AK positive on PYG growth media, and 9 specimens were found AK positive on TYI-S-33 growth media. Only five specimens were found AK positive on NNA growth media. Therefore, we recommend using fluorescence microscopy technique and culture method, especially PYG liquid media.

  16. Effects of embryo culture media do not persist after implantation: a histological study in mice

    NARCIS (Netherlands)

    Hemkemeyer, Sandra A.; Schwarzer, Caroline; Boiani, Michele; Ehmcke, Jens; le Gac, Severine; Schlatt, Stefan; Nordhoff, Verena

    2014-01-01

    Is post-implantation embryonic development after blastocyst transfer affected by exposure to different assisted reproduction technology (ART) culture media? Fetal development and placental histology of ART embryos cultured in vitro in different ART media was not impaired compared with embryos grown

  17. 9 CFR 113.25 - Culture media for detection of bacteria and fungi.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Culture media for detection of bacteria and fungi. 113.25 Section 113.25 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION... STANDARD REQUIREMENTS Standard Procedures § 113.25 Culture media for detection of bacteria and fungi. (a...

  18. The Role of Social Media in Higher Education : Case KTUAS Faculty of Business and Culture

    OpenAIRE

    Mattila, Katja

    2012-01-01

    Mattila, Katja. 2012. The Role of Social Media in Higher Education: Case KTUAS Faculty of Business and Culture. Master’s Thesis. Kemi-Tornio University of Applied Sciences. Business and Culture. Pages 72. Appendices 1 - 3. The main objective of this thesis is to delineate the role and implications of the use of social media in educational work in higher education as well as discuss the influences social media use has on the everyday work within the case organization. The case organization ...

  19. Social media in higher education: A look at participatory culture in graduate coursework

    OpenAIRE

    Ann-Louise Davidson; Renee-Marie Fountain

    2013-01-01

    Society has become fascinated with web- based social media. Recently, aspects of social media environments such as participatory culture, new media digital literacies, and connectivism have been increasingly investigated. However, current university policies often restrict, if not forbid, the use of social networking sites in class. For professors seeking to introduce social media into their teaching practice, these restrictive policies can make it difficult to teach with and about social com...

  20. [Cultural components within DSM-5: achievements, hopes, and expectations].

    Science.gov (United States)

    Alarcón, Renato D

    2014-01-01

    Cultural Psychiatry deals with the description, definition, evaluation and management of psychiatric conditions as a clinical reflection of cultural factors within an integral context, and as an explanatory, interpretative, nosological, therapeutic and preventive attribute in professional practice. This article attempts to analyze that link in the context of the dominant classification in our era, the Diagnostic and Statistical Manual of Mental Disorders (DSM) of the American Psychiatric Association (APA), with emphasis on the development of its latest version, DSM-5. The cultural content of the International Classification of Diseases (ICD) of the World Health Organization (WHO) can be the subject of further analysis, even when it can be said that, in general, it seemingly has less reach than the American classification. The author's participation, work and reflections about the DSM-5 Committee, created by the APA at the beginning of this century, constitute the basis of the presentation and discussion of concrete achievements, more or less idealized hopes, and more or less realistic expectations with a view to the future. Conclusions will also try to cover implications of DSM-5 cultural components in the field of Latin American and spanish-speaking psychiatry.

  1. New dimensions of identity – media, popular culture, digital reality

    Directory of Open Access Journals (Sweden)

    Wojciech Baluch

    2016-06-01

    Full Text Available The text concentrates on the issue of identity and its transformations, driven by the spread of media and digital culture. In the first part it presents three canonic proposals for reflections on virtual identity. Firstly, reflections on the communication dimension of identity derived from Martin Buber’s philosophy of dialog. Secondly, the concept of “ontological shift” as suggested by Marek Krajewski, who sees in it the remedy for the lack of a real dimension of many virtual contacts. Thirdly, the issues of the integrity of the individual and the fluidity of modern reality in the approaches proposed by Wolfgang Welsh, Anthony Giddens, and Zygmunt Bauman. The analytical part contains an analysis of the important aspects of the notion of identity. The first is the issue of the subjectivity of an utterance and its source, which will be discussed using the example of a play based on texts that adapted from Internet blogs. The second issue is an attempt to answer the question posed by Sherry Turkle of whether if it is possible to kill someone virtually. The point of reference for the search for a solution to this issue is the popular computer game “Vampire”.The last aspect has to do with celebrity identity in the media world. This concerns the phenomenon of celebrities in the pragmatic dimension as a model strategy of behaviour in the world, in which the boundary between privacy and public space has been blurred. On the basis of these analyses, which cover merely a small part of human experience, the author proposes a new methodological approach to perceiving and analysing aspects of identity revealed through the confrontation with the new virtual reality.

  2. Media Consumption on the World Wide Web: Integrating Theories of Media Choice and Global Media Flows to Explain Global Cultural Consumption

    Science.gov (United States)

    Taneja, Harsh

    2014-01-01

    The cross border availability of media content has raised speculations that content preferences would largely drive audience choices. In such a scenario, technologies and institutional structures would primarily shape patterns of global cultural consumption, sweeping away old allegiances based on cultural traits such as language and geography. On…

  3. Effect of Media Culture on Growth and Sucker Pandanus Plant

    Directory of Open Access Journals (Sweden)

    ali salehi sardoei

    2017-02-01

    Full Text Available Introduction: One factor that is of great importance to the cultivation of flowers and ornamental plants, is the media. Planting plants in containers as an important component of the nursery technology has grown. Compared with farm volume, growth media used for each plant greatly reduce plant growth that largely influence by the physical and chemical properties of growth media used. Therefore, good management of potted plants bed will cause the plants have good quality. A good growth media with optimal physical and biological properties, relatively inexpensive, stable and style enough to work should be available. The Burgers showed that composted green waste can be used as substrates for soilless cultivation and improve the water-holding capacity of soil. The garden has a range of materials including hardwood and softwood bark, leaves, soil, waste, sewage sludge and coconut (cocopeat that has been used as a seed bed. According to the economic issues and increasing moisture storage, palm peat substrates are primary material that can be prepared as a good growth medium for the producing's presented level Country. Peat moss is not applicable to all plants because of high cost and poor absorption characteristics like low pH and low water holding capacity . This study was conducted to investigate the possibility of replacing peat moss palm waste and the effect of it on growth characteristics were studied. Materials and Methods: The experimental design was completely randomized design with four replications of eight treatments. The compressed unit (block was supplied and commercial cocopeat was used because of reducing the cost of transportation. Before applying this material, the amount of water was added for opening up and voluminous and become it completely uniform.. In treatments containing sand + perlite, these four types volume ratio of 1:1 and mixed with sand + perlite were used. First, wooden cuttings of pandanus in a bed of sand rooted in the

  4. Factors Influencing Social Media Marketing In Different Culture Context.

    OpenAIRE

    Omar, Juwayria

    2014-01-01

    Social media has gained precedence in today‟s business environment, and consumers themselves are more receptive to this marketing media. This study aims to identify the factors affecting users‟ attitudes towards social media marketing. From the literature review, a conceptual model was proposed, and five hypotheses were developed. The model studies the effect of several independent variables on attitude towards social media marketing. A questionnaire was completed by students from Norway and ...

  5. Factors Influencing Social Media Marketing In Different Culture Context.

    OpenAIRE

    Omar, Juwayria

    2014-01-01

    Social media has gained precedence in today‟s business environment, and consumers themselves are more receptive to this marketing media. This study aims to identify the factors affecting users‟ attitudes towards social media marketing. From the literature review, a conceptual model was proposed, and five hypotheses were developed. The model studies the effect of several independent variables on attitude towards social media marketing. A questionnaire was completed by students from Norway and ...

  6. Communication and Cultural Memory in Contemporary Tourism Media Products: Culture-specific and Cross-cultural Perspectives

    Directory of Open Access Journals (Sweden)

    Aleksandra Salamurović

    2015-08-01

    Full Text Available Communication practices which are a part of the contemporary media-culture are intrinsically tied to the processes of (recreating collective identities. One of the possible strategies in the frame of the mediated communication practice is to connect traditional ele-ments of cultural memory with new ones, which are declared as preferable and acceptable. In that way the collective identity remains, on the one hand, “homoge-neous”, offering stability to the members of communica-tion community, on the other hand, it is subject to change and dynamics, always “ready” to be reshaped in order to achieve wider acceptance. The tourism media products, especially tourism promotion videos, are the best examples for this mediated communication prac-tice. The visual images, combined with text messages, i.e. slogans, are not only some of the most important narrative mechanisms in the presentation of certain tourist destination, they are also the key elements of the mediated collective cultural memory and identity of the respective country presented in the tourism promotion videos. The main goal of this article is to examine the represen-tation and composition forms of some of the tourism promotion videos both from the Balkan countries as well as from other regions worldwide related especially to the elements of the cultural memory in order to de-fine culture-specific and cross-cultural strategies rele-vant to the creation of the collective identity. The analy-sis is based on the Critical Discourse Analysis, respec-tively the analytical framework of the “Grammar of Vis-ual Design” by Kress/van Leeuwen.

  7. Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media.

    Science.gov (United States)

    Jozala, Angela Faustino; Pértile, Renata Aparecida Nedel; dos Santos, Carolina Alves; de Carvalho Santos-Ebinuma, Valéria; Seckler, Marcelo Martins; Gama, Francisco Miguel; Pessoa, Adalberto

    2015-02-01

    Bacterial cellulose (BC) is used in different fields as a biological material due to its unique properties. Despite there being many BC applications, there still remain many problems associated with bioprocess technology, such as increasing productivity and decreasing production cost. New technologies that use waste from the food industry as raw materials for culture media promote economic advantages because they reduce environmental pollution and stimulate new research for science sustainability. For this reason, BC production requires optimized conditions to increase its application. The main objective of this study was to evaluate BC production by Gluconacetobacter xylinus using industry waste, namely, rotten fruits and milk whey, as culture media. Furthermore, the structure of BC produced at different conditions was also determined. The culture media employed in this study were composed of rotten fruit collected from the disposal of free markets, milk whey from a local industrial disposal, and their combination, and Hestrin and Schramm media was used as standard culture media. Although all culture media studied produced BC, the highest BC yield-60 mg/mL-was achieved with the rotten fruit culture. Thus, the results showed that rotten fruit can be used for BC production. This culture media can be considered as a profitable alternative to generate high-value products. In addition, it combines environmental concern with sustainable processes that can promote also the reduction of production cost.

  8. Quantifying the Economic and Cultural Biases of Social Media through Trending Topics.

    Science.gov (United States)

    Carrascosa, Juan Miguel; Cuevas, Ruben; Gonzalez, Roberto; Azcorra, Arturo; Garcia, David

    2015-01-01

    Online social media has recently irrupted as the last major venue for the propagation of news and cultural content, competing with traditional mass media and allowing citizens to access new sources of information. In this paper, we study collectively filtered news and popular content in Twitter, known as Trending Topics (TTs), to quantify the extent to which they show similar biases known for mass media. We use two datasets collected in 2013 and 2014, including more than 300.000 TTs from 62 countries. The existing patterns of leader-follower relationships among countries reveal systemic biases known for mass media: Countries concentrate their attention to small groups of other countries, generating a pattern of centralization in which TTs follow the gradient of wealth across countries. At the same time, we find subjective biases within language communities linked to the cultural similarity of countries, in which countries with closer cultures and shared languages tend to follow each other's TTs. Moreover, using a novel methodology based on the Google News service, we study the influence of mass media in TTs for four countries. We find that roughly half of the TTs in Twitter overlap with news reported by mass media, and that the rest of TTs are more likely to spread internationally within Twitter. Our results confirm that online social media have the power to independently spread content beyond mass media, but at the same time social media content follows economic incentives and is subject to cultural factors and language barriers.

  9. Quantifying the Economic and Cultural Biases of Social Media through Trending Topics

    Science.gov (United States)

    Carrascosa, Juan Miguel; Cuevas, Ruben; Gonzalez, Roberto; Azcorra, Arturo; Garcia, David

    2015-01-01

    Online social media has recently irrupted as the last major venue for the propagation of news and cultural content, competing with traditional mass media and allowing citizens to access new sources of information. In this paper, we study collectively filtered news and popular content in Twitter, known as Trending Topics (TTs), to quantify the extent to which they show similar biases known for mass media. We use two datasets collected in 2013 and 2014, including more than 300.000 TTs from 62 countries. The existing patterns of leader-follower relationships among countries reveal systemic biases known for mass media: Countries concentrate their attention to small groups of other countries, generating a pattern of centralization in which TTs follow the gradient of wealth across countries. At the same time, we find subjective biases within language communities linked to the cultural similarity of countries, in which countries with closer cultures and shared languages tend to follow each other’s TTs. Moreover, using a novel methodology based on the Google News service, we study the influence of mass media in TTs for four countries. We find that roughly half of the TTs in Twitter overlap with news reported by mass media, and that the rest of TTs are more likely to spread internationally within Twitter. Our results confirm that online social media have the power to independently spread content beyond mass media, but at the same time social media content follows economic incentives and is subject to cultural factors and language barriers. PMID:26230656

  10. Quantifying the Economic and Cultural Biases of Social Media through Trending Topics.

    Directory of Open Access Journals (Sweden)

    Juan Miguel Carrascosa

    Full Text Available Online social media has recently irrupted as the last major venue for the propagation of news and cultural content, competing with traditional mass media and allowing citizens to access new sources of information. In this paper, we study collectively filtered news and popular content in Twitter, known as Trending Topics (TTs, to quantify the extent to which they show similar biases known for mass media. We use two datasets collected in 2013 and 2014, including more than 300.000 TTs from 62 countries. The existing patterns of leader-follower relationships among countries reveal systemic biases known for mass media: Countries concentrate their attention to small groups of other countries, generating a pattern of centralization in which TTs follow the gradient of wealth across countries. At the same time, we find subjective biases within language communities linked to the cultural similarity of countries, in which countries with closer cultures and shared languages tend to follow each other's TTs. Moreover, using a novel methodology based on the Google News service, we study the influence of mass media in TTs for four countries. We find that roughly half of the TTs in Twitter overlap with news reported by mass media, and that the rest of TTs are more likely to spread internationally within Twitter. Our results confirm that online social media have the power to independently spread content beyond mass media, but at the same time social media content follows economic incentives and is subject to cultural factors and language barriers.

  11. Transformative Power of Digital Citizenship: Critical Perspectives on Culture, New Media and Pedagogy

    Science.gov (United States)

    Kurubacak, Gulsun

    2007-01-01

    This paper discusses culture, as a source of conflict than of synergy, how affects the use of new media to build digital citizenships. It also argues that the cultural dimensions of Geert Hofstede, who demonstrates that there are national and regional cultural groupings that affect the behavior of organizations, are very persistent across time.…

  12. The role of culture media on embryonation and subsequent infectivity of Capillaria obsignata eggs.

    Science.gov (United States)

    Tiersch, K M; Daş, G; Samson-Himmelstjerna, G V; Gauly, M

    2013-01-01

    This study investigated whether infectivity of Capillaria obsignata eggs depends on media culture used for embryonation. Intact female worms were kept in one of following four media: 0.5 % formalin, 2 % formalin, 0.1 % potassium dichromate and 0.1 N sulfuric acid. Embryonation rates of the eggs were quantified either daily in intact females for 16 days, or weekly in disrupted females. Infectivity of the embryonated eggs was tested through an experimental infection of chickens with a single dose of 250 eggs/ bird. The vast majority of the eggs (>82 %) in the first two thirds of the uteri was able to complete embryonation, irrespective of the culture media used for incubation. However, only 32.6 % of total eggs could be harvested after disruption of the intact females. Embryonation rates of the eggs from disrupted worms were different among four culture media, with 0.1 N sulfuric acid resulting in the highest embryonation rate (44.2 %). All the experimentally infected birds harboured mature worms, with varying establishment rates depending on the culture media (P media. However, disruption of the intact females results in lower number of harvestable embryonated eggs, with a considerable variation due to culture media used. With the exception of 0.1 % potassium dichromate, any of the three media, particularly 0.1 N sulfuric acid, can be suggested for embryonation of C. obsignata eggs.

  13. Digital Culture and Social Media versus the Traditional Education

    OpenAIRE

    Agim Poshka

    2014-01-01

    This article aims to reflect on the increasing momentum that social media have in the everyday life our students and to investigate the uniqueness that this media offers to the process of education. The study investigates the benefits that Facebook and Twitter have as the leading technologically mediated spaces and its application to the learning habitat of the learner in the public pedagogy. The article reflects on the opportunities that social media offers in order to avoid the self-created...

  14. Cultural Resiliency and the Rise of Indigenous Media

    Directory of Open Access Journals (Sweden)

    Derek Moscato

    2016-04-01

    Full Text Available Valerie Alia’s book, The New Media Nation: Indigenous Peoples and Global Communication (New York: Berghahn Books, 2012, 270 pp., points the way to major communication breakthroughs for traditional communities around the world, in turn fostering a more democratic media discourse. From Canada to Japan, and Australia to Mexico, this ambitious and wide-reaching work examines a broad international movement that at once protects ancient languages and customs but also communicates to audiences across countries, oceans, and political boundaries. The publication is divided roughly into five sections: The emergence of a global vision for Indigenous communities scattered around the world; government policy obstacles and opportunities; lessons from Canada, where Indigenous media efforts have been particularly dynamic; the global surge in television, radio and other technological media advances; and finally the long-term prospects and aspirations for Indigenous media. By laying out such a comprehensive groundwork for the rise of global Indigenous media over a variety of formats, particularly over the past century, Alia shows how recent social media breakthroughs such as the highly successful #IdleNoMore movement—a sustained online protest by Canada’s First Nations peoples—have been in fact inevitable. The world’s Indigenous communities have leveraged media technologies to overcome geographic isolation, to foster new linkages with Indigenous populations globally, and ultimately to mitigate structural power imbalances exacerbated by non-Indigenous media and other institutions.

  15. Cultural Resiliency and the Rise of Indigenous Media

    Directory of Open Access Journals (Sweden)

    Derek Moscato

    2016-04-01

    Full Text Available Valerie Alia’s book, The New Media Nation: Indigenous Peoples and Global Communication (New York: Berghahn Books, 2012, 270 pp., points the way to major communication breakthroughs for traditional communities around the world, in turn fostering a more democratic media discourse. From Canada to Japan, and Australia to Mexico, this ambitious and wide-reaching work examines a broad international movement that at once protects ancient languages and customs but also communicates to audiences across countries, oceans, and political boundaries. The publication is divided roughly into five sections: The emergence of a global vision for Indigenous communities scattered around the world; government policy obstacles and opportunities; lessons from Canada, where Indigenous media efforts have been particularly dynamic; the global surge in television, radio and other technological media advances; and finally the long-term prospects and aspirations for Indigenous media. By laying out such a comprehensive groundwork for the rise of global Indigenous media over a variety of formats, particularly over the past century, Alia shows how recent social media breakthroughs such as the highly successful #IdleNoMore movement—a sustained online protest by Canada’s First Nations peoples—have been in fact inevitable. The world’s Indigenous communities have leveraged media technologies to overcome geographic isolation, to foster new linkages with Indigenous populations globally, and ultimately to mitigate structural power imbalances exacerbated by non-Indigenous media and other institutions.

  16. Silver nanoparticle protein corona composition in cell culture media.

    Directory of Open Access Journals (Sweden)

    Jonathan H Shannahan

    Full Text Available The potential applications of nanomaterials as drug delivery systems and in other products continue to expand. Upon introduction into physiological environments and driven by energetics, nanomaterials readily associate proteins forming a protein corona (PC on their surface. This PC influences the nanomaterial's surface characteristics and may impact their interaction with cells. To determine the biological impact of nanomaterial exposure as well as nanotherapeutic applications, it is necessary to understand PC formation. Utilizing a label-free mass spectrometry-based proteomics approach, we examined the composition of the PC for a set of four silver nanoparticles (AgNPs including citrate-stabilized and polyvinlypyrrolidone-stabilized (PVP colloidal silver (20 or 110 nm diameter. To simulate cell culture conditions, AgNPs were incubated for 1 h in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum, washed, coronal proteins solubilized, and proteins identified and quantified by label-free LC-MS/MS. To determine which attributes influence PC formation, the AgNPs were characterized in both water and cell culture media with 10% FBS. All AgNPs associated a common subset of 11 proteins including albumin, apolipoproteins, keratins, and other serum proteins. 110 nm citrate- and PVP-stabilized AgNPs were found to bind the greatest number of proteins (79 and 85 respectively compared to 20 nm citrate- and PVP-stabilized AgNPs (45 and 48 respectively, suggesting a difference in PC formation based on surface curvature. While no relationships were found for other protein parameters (isoelectric point or aliphatic index, the PC on 20 nm AgNPs (PVP and citrate consisted of more hydrophobic proteins compared to 110 nm AgNPs implying that this class of proteins are more receptive to curvature-induced folding and crowding in exchange for an increased hydration in the aqueous environment. These observations demonstrate the significance of

  17. Social Media and eBusiness: Cultural Impacts on the Influence Process in Consumer Communities

    Science.gov (United States)

    Chen, Yong; Chen, Hong; Xu, Li

    2016-08-01

    Social media has been used as an important tool by firms to influence consumers’ attitude and behavior. Influence occurs in consumer communities in social media because community members have the control of discovering, producing, sharing, and distributing information and because the spread out of their experiences and opinions in the format of electronic word-of-mouth forms emerging conformance. Prior research has explored how the influence occurring in online social media communities impacts consumers’ attitude and behavior (e.g., product attitude and purchase decision, effectual thinking and behavior, brand trust and brand loyalty). But because social media has the ability of global reach, cross-border factors should not be neglected in studying the influence process. As such, this paper adopts national cultural dimensions identified by Hofstede (1984), individualism/collectivism and power distance particularly, the index of cultural distance, and the social influence theory to explore how culture impacts the influence occurring in consumer communities in social media.

  18. Remote Control Childhood? Combating the Hazards of Media Culture.

    Science.gov (United States)

    Levin, Diane E.

    Ever since television became a daily staple of U.S. family life, its influence on children has been the subject of study and debate. No aspect of the debate has been more heated than violence in the media. But a growing knowledge base has shifted the focus of the debate from whether media violence contributes to violence in real life to what can…

  19. The New Audiovisual Media Services Directive : Television without Frontiers, Television without Cultural Diversity

    OpenAIRE

    Burri, Mira

    2007-01-01

    After long deliberations, the European Community (EC) has completed the reform of its audiovisual media regulation. The paper examines the main tenets of this reform with particular focus on its implications for the diversity of cultural expressions in the European media landscape. It also takes into account the changed patterns of consumer and business behaviour due to the advances in digital media and their wider spread in society. The paper criticises the somewhat unimaginative approach of...

  20. Film production, social media marketing and participatory culture

    DEFF Research Database (Denmark)

    Waade, Anne Marit

    The Danish youth film ‘Lev Stærkt’ (Live strong) is recently shot in Aarhus, and as part of the release and marketing plan, the producers incorporate social media showing behind the scenes video clip as a way to include and engage the film’s target groups a year before the planned release. Using...... tendency within film and TV industry, in which behind the scene clips and comments are used in advantage to promote the product, as well as using social media as the main marketing channel (Caldwell, 2008; Gray, 2010; Johnson, 2012). Social media marketing is in itself representing a new field within...... branding and marketing, and there is a boom of new handbook literature describing “Everything You Need to Know to Get Social Media Working in Your Business” (Wollan & Nick Zhou, 2010). Social media makes it easy to engage the consumers as strategic communicators, it is cheap and fast compared to print...

  1. Film production, social media marketing and participatory culture

    DEFF Research Database (Denmark)

    Waade, Anne Marit

    tendency within film and TV industry, in which behind the scene clips and comments are used in advantage to promote the product, as well as using social media as the main marketing channel (Caldwell, 2008; Gray, 2010; Johnson, 2012). Social media marketing is in itself representing a new field within......The Danish youth film ‘Lev Stærkt’ (Live strong) is recently shot in Aarhus, and as part of the release and marketing plan, the producers incorporate social media showing behind the scenes video clip as a way to include and engage the film’s target groups a year before the planned release. Using...... branding and marketing, and there is a boom of new handbook literature describing “Everything You Need to Know to Get Social Media Working in Your Business” (Wollan & Nick Zhou, 2010). Social media makes it easy to engage the consumers as strategic communicators, it is cheap and fast compared to print...

  2. Folk Culture Resources as a Component of Tourism Space

    OpenAIRE

    Mokras-Grabowska, Justyna

    2014-01-01

    The paper concerns folk tourism - describes the mutual relations between folk culture and tourism and the main mechanisms of the commercialization of cultural heritage. Moreover it locates folk culture resources in tourism space and includes hospitality.

  3. "Japanese Patterns of Paticipation in Cultural and Media-related Activities"

    OpenAIRE

    Ikeguchi, Cecilia

    2001-01-01

    Using two groups of samples,this investigation looks into the patterns of interaction between reading books,as a communications media,and various modes of cultural behabiour such as theater,concerts,museums and attending lectures ...

  4. The Impact of Social Media Enterprise Crowdsourcing on Company Innovation Culture

    DEFF Research Database (Denmark)

    Hugger, Ada Scupola; Nicolajsen, Hanne Westh

    2012-01-01

    In this article we investigate how social media-based crowdsourcing systems can be used to reengineer the innovation culture in an organization. Based on a case study of a large engineering consultancy's use of a social media crowdsourcing system we investigate the impact on the organizations...

  5. A Cross-Cultural Comparison of Domestic American and International Chinese Students' Social Media Usage

    Science.gov (United States)

    Xu, Qiong; Mocarski, Richard

    2014-01-01

    This survey of American and Chinese students at a state university in the southern United States measures Social Media (SM) use and attitudes toward SM. The purpose of this study was to investigate student perception and motivation of social media communication and the relationship between student cultural values and their social media…

  6. Tissue culture media supplemented with 10% fetal calf serum contains a castrate level of testosterone.

    NARCIS (Netherlands)

    Sedelaar, J.P.M.; Isaacs, J.T.

    2009-01-01

    BACKGROUND: Human prostate cancer cells are routinely maintained in media supplemented with 10% Fetal Calf Serum (FCS) to provide androgen. In the present study, total and free testosterone levels in 10%FCS supplemented tissue culture media were determined and compared to levels in intact and

  7. Developmentally Appropriate New Media Literacies: Supporting Cultural Competencies and Social Skills in Early Childhood Education

    Science.gov (United States)

    Alper, Meryl

    2013-01-01

    Young children explore their world through manipulatives, playing with "technology" that may or may not be digital. To this end, I offer an exploration into how the existing framework of the New Media Literacies (NMLs) paradigm set forth by Henry Jenkins (2006) in "Confronting the Challenges of Participatory Culture: Media Education…

  8. Understanding Social Media Culture and its Ethical Challenges for Art Therapists

    Science.gov (United States)

    Belkofer, Christopher M.; McNutt, Jill V.

    2011-01-01

    This article discusses ethics in the context of the participatory culture of social media as it relates to art therapy. The authors present the view that social media formats are important venues for expression that contribute to interpersonal connections and social learning via the active participation of their members. To make informed ethical…

  9. Cultural Diversity in the News Media: A Democratic or a Commercial Need?

    NARCIS (Netherlands)

    I. Awad Cherit (Isabel)

    2008-01-01

    textabstractThis paper distinguishes between laissez-faire and interventionist models used to justify and implement cultural diversity initiatives in the news media. The laissez-faire model is characteristic of U.S journalism. However, due to the convergence of media systems and the widespread adopt

  10. Developmentally Appropriate New Media Literacies: Supporting Cultural Competencies and Social Skills in Early Childhood Education

    Science.gov (United States)

    Alper, Meryl

    2013-01-01

    Young children explore their world through manipulatives, playing with "technology" that may or may not be digital. To this end, I offer an exploration into how the existing framework of the New Media Literacies (NMLs) paradigm set forth by Henry Jenkins (2006) in "Confronting the Challenges of Participatory Culture: Media Education…

  11. Innovative use of recovered municipal solid waste incineration bottom ash as a component in growing media.

    Science.gov (United States)

    Sormunen, Annika; Teo, Kanniainen; Tapio, Salo; Riina, Rantsi

    2016-07-01

    The utilisation of municipal solid waste incineration bottom ash has been extensively studied, for example, in the unbound layers of roads and the products of cement and concrete industry. On the other hand, less attention has been given to other innovative utilisation possibilities, such as using the municipal solid waste incineration bottom ash as a component in growing media of plants. The municipal solid waste incineration bottom ash contains useful substances, such as calcium, that can influence plant growth in a positive manner. Therefore, the utilisation of this waste-derived material in the growing media may substitute the use of commercial fertilisers. Since the municipal solid waste incineration bottom ash also contains hazardous substances that can be toxic to plants, the main aim of this study was to add different amounts of recovered municipal solid waste incineration bottom ash in the growing media and to evaluate the effect of this material on plant growth. Based on the obtained results, the concentration of, for example copper and zinc, increased in test plants; ryegrass and barley, when recovered municipal solid waste incineration bottom ash was added in their growing media. On the other hand, this did not have a significant effect on plant growth, if compared with the growth of plants in commercially produced growing medium. Furthermore, the replacement of natural sand with municipal solid waste incineration bottom ash had a positive liming effect in the growing media. Overall, these findings suggest that the utilisation of recovered municipal solid waste incineration bottom ash as a component in growing media is possible and, thus, may allow more widespread and innovative use of this waste-derived material. © The Author(s) 2016.

  12. Investigating the Role of Sport Media in Development and Promotion of Components of Physical Readiness Sport

    Directory of Open Access Journals (Sweden)

    Mehdi Moradi

    2012-06-01

    Full Text Available Given the undeniable role of sport media in shaping and giving direction to the beliefs, attitudes and thought of society and also the important role of physical activities and physical readiness in the physical and mental health of society and reducing therapeutic costs, the issue that rose is that sport media to what extent partake in development and promotion the components of physical readiness? The research methodology is descriptive-comparative, the type of the research was applied, the method of accomplishment was survey, and data collection was field study. Research Population was the sports and media experts. Research sample included 140 people and the sampling method was non-random. Descriptive statistics was used to describe the personal specifications of examinees. Kolmogorov-Smirnov test was used to investigate if the data is normal and Wilcoxon’s test was used to determine the significance of hypotheses. A researcher-made questionnaire including 60 items in 10 dimensions and 5-points Likert spectrum was used to collect data. The validity of the questionnaire was determined by 15 academics and experts in the field of communications, physical education and sport. Its reliability is also determined through Cronbach's Alpha (α=0.84. From the viewpoint of sport and media experts, there is significant difference between status quo and desired status of the role of sport media in developing and promoting the components of physical readiness in level P=0.05. From the viewpoints of examinees, the lack of universality in sport media’s programs and the lack of recognition in media people about the various dimensions of athletic sports are the main reasons for the difference between status quo and desired status.

  13. "Rape Culture" language and the news media: contested versus non-contested cases

    Directory of Open Access Journals (Sweden)

    April COBOS

    2014-12-01

    Full Text Available The American news media has recently reported on several rape and sexual assault cases in various cultural settings, sparking public conversations about rape culture in different cultural contexts. The article is focused as a Critical Discourse Analysis that compares the language use in news articles from The New York Times and The Wall Street Journal over a six months period in order to more clearly understand the way the news media uses language in regards to gender and sexual assault and creates a spectrum of valid versus contested reports of sexual assault in different cultural settings.

  14. The Impact of Social Media Enterprise Crowdsourcing on Company Innovation Culture

    DEFF Research Database (Denmark)

    Hugger, Ada Scupola; Nicolajsen, Hanne Westh

    2012-01-01

    innovation culture using theory on organizational culture and crowdsourcing. The analysis shows that the organizational crowdsourcing event has supported an innovation culture change in the case company towards a more open approach to innovation; creating a new and different awareness of innovation, allowing......In this article we investigate how social media-based crowdsourcing systems can be used to reengineer the innovation culture in an organization. Based on a case study of a large engineering consultancy's use of a social media crowdsourcing system we investigate the impact on the organizations...

  15. The Impact of Social Media Enterprise Crowdsourcing on Company Innovation Culture

    DEFF Research Database (Denmark)

    Hugger, Ada Scupola; Nicolajsen, Hanne Westh

    2012-01-01

    In this article we investigate how social media-based crowdsourcing systems can be used to reengineer the innovation culture in an organization. Based on a case study of a large engineering consultancy's use of a social media crowdsourcing system we investigate the impact on the organizations...... innovation culture using theory on organizational culture and crowdsourcing. The analysis shows that the organizational crowdsourcing event has supported an innovation culture change in the case company towards a more open approach to innovation; creating a new and different awareness of innovation, allowing...

  16. The Impact of Social Media and Crowdsourcing on Organizational Innovation Culture

    DEFF Research Database (Denmark)

    Scupola, Ada; Nicolajsen, Hanne Westh

    In this article we investigate how social media-based crowdsourcing systems can be used to reengineer the innovation culture in an organization. Based on a case study of a large engineering consultancy’s use of a social media crowdsourcing system we investigate the impact on the organizations...... innovation culture using theory on organizational culture and crowdsourcing. The analysis shows that the organizational crowdsourcing event has supported an innovation culture change in the case company towards a more open approach to innovation; creating a new and different awareness of innovation, allowing...

  17. Mídia, juventude e memória cultural Media, youth and cultural memory

    Directory of Open Access Journals (Sweden)

    Rosa Maria Bueno Fischer

    2008-10-01

    Full Text Available O texto trata das relações entre memória, mídia e juventude. Discute os resultados de uma pesquisa com estudantes universitários e de ensino médio, sobre suas memórias culturais e midiáticas, problematizando questões contemporâneas sobre alteridade, memória e produções audiovisuais, a partir de autores como Henri Bergson, Andreas Huyssen, Maria Rita Kehl e Michel Foucault, entre outros. Desenvolve-se o argumento de que a produção de sujeitos, em nosso tempo, estaria estreitamente relacionada à experiência cotidiana, em particular dos mais jovens, com as imagens e textos oferecidos pelos meios tecnológicos de informação e comunicação. Estes meios, segundo a argumentação tecida, parecem operar fortemente nos processos de elaboração de nossas memórias individuais e sociais, bem como na construção de modos de existência específicos, relacionados à construção de nós mesmos e de nossas diferenças.This text aims to discuss relations between memory, media and youth. I discuss dates from a research with Brazilian students about their cultural memories, alterity and audiovisual products in our culture. Theoretical references are concepts from Henri Bergson, Andreas Huyssen, Maria Rita Kehl and Michel Foucault. I put forward the argument that production of subjectivity, in our times, is narrowly related to the experience with images and texts from different media. Technologies of communication and information offer an important source to memories construction, in order to shape our lives and our differences.

  18. mRNA fragments in in vitro culture media are associated with bovine preimplantation embryonic development.

    Science.gov (United States)

    Kropp, Jenna; Khatib, Hasan

    2015-01-01

    In vitro production (IVP) systems have been used to bypass problems of fertilization and early embryonic development. However, embryos produced by IVP are commonly selected for implantation based on morphological assessment, which is not a strong indicator of establishment and maintenance of pregnancy. Thus, there is a need to identify additional indicators of embryonic developmental potential. Previous studies have identified microRNA expression in in vitro culture media to be indicative of embryo quality in both bovine and human embryos. Like microRNAs, mRNAs have been shown to be secreted from cells into the extracellular environment, but it is unknown whether or not these RNAs are secreted by embryos. Thus, the objective of the present study was to determine whether mRNAs are secreted into in vitro culture media and if their expression in the media is indicative of embryo quality. In vitro culture medium was generated and collected from both blastocyst and degenerate (those which fail to develop from the morula to blastocyst stage) embryos. Small-RNA sequencing revealed that many mRNA fragments were present in the culture media. A total of 17 mRNA fragments were differentially expressed between blastocyst and degenerate conditioned media. Differential expression was confirmed by quantitative real-time PCR for fragments of mRNA POSTN and VSNL-1, in four additional biological replicates of media. To better understand the mechanisms of mRNA secretion into the media, the expression of a predicted RNA binding protein of POSTN, PUM2, was knocked down using an antisense oligonucleotide gapmer. Supplementation of a PUM2 gapmer significantly reduced blastocyst development and decreased secretion of POSTN mRNA into the media. Overall, differential mRNA expression in the media was repeatable and sets the framework for future study of mRNA biomarkers in in vitro culture media to improve predictability of reproductive performance.

  19. Effects of Mass Media and Cultural Drift in a Nonequilibrium Model for Social Influence

    CERN Document Server

    Mazzitello, K I; Dossetti, V; Candia, Juli\\'an; Mazzitello, Karina I.

    2006-01-01

    In the context of an extension of Axelrod's nonequilibrium model for social influence, we study the interplay and competition between the cultural drift, represented as random perturbations, and mass media, introduced by means of an external homogeneous field. Unlike previous studies [J. C. Gonz\\'alez-Avella {\\it et al}, Phys. Rev. E {\\bf 72}, 065102(R) (2005)], the mass media coupling proposed here is capable of affecting the cultural traits of any individual in the society, including those who do not share any features with the external message. A noise-driven transition is found: for large noise rates, both the ordered (culturally polarized) phase and the disordered (culturally fragmented) phase are observed, while, for lower noise rates, the ordered phase prevails. In the former case, the external field is found to induce cultural ordering, a behavior opposite to that reported in previous studies using a different prescription for the mass media interaction. We compare the predictions of this model to sta...

  20. Chromogenic media for urine cultures can be cost-effective

    Directory of Open Access Journals (Sweden)

    Matjaž J. Retelj

    2007-03-01

    Full Text Available Background: Chromogenic media for diagnostic urinary bacteriology have several advantages over traditional media, such as cysteine-lactose-electrolyte deficient (CLED medium. Chromogenic media allow for easier recognition of mixed growth, save time, reduce workload and provide higher detection rates. However, the cost of chromogenic media is significantly higher compared to CLED and performance of chromogenic media varies depending on the manufacturer. In the present study, performance, turn-around time and cost of Uriselect4 chromogenic medium was compared to CLED.Methods: For performance analysis, 351 midstream urine (MSU samples from September 2005 to December 2005 were directly plated in parallel on Uriselect4 and CLED agar using the calibrated loop technique. Isolates on Uriselect4 were presumptively identified according to the product insert. For cost-effectiveness analysis, we included 1,972 consecutive MSU samples from May 2005 to July 2006. We compared the cost of required materials as well as technologists’ or specialists’ time for each medium examined.Results: No significant differences were found between the isolation rates of urinary pathogens on the studied media. The procedure using chromogenic media for uropathogens is slightly cheaper than the procedure using CLED, considering the proportion of bacteriuria positive samples (50.5 % and the distribution of taxa among isolates (namely Escherichia coli with 59.6 % observed in our laboratory. At the current isolation proportion in MSU samples processed in our laboratory, the average time to reporting results could be decreased by 0.3 days.Conclusions: Use of chromogenic media for urine investigations offers multiple advantages without increasing costs compared to procedures using CLED.

  1. Cold Culture: Polar Media and the Nazi Occult

    OpenAIRE

    Krapp, Peter

    2009-01-01

    The inaccessibility of the North and South Pole makes them a crucible for persistent questions of access and data visualization that characterize the information age. Arctic and Antarctic have become increasingly topical in popular cinema as well as in media arts. As representations of polar regions grapple with the fictions that mark representations of science, they illustrate the perils and perks of polar travel in the age of digital media. This essay sets out to trace representations of th...

  2. Cultured meat in western media:The disproportionate coverage of vegetarian reactions, demographic realities, and implications for cultured meat marketing

    Institute of Scientific and Technical Information of China (English)

    Patrick D Hopkins

    2015-01-01

    This paper examines the media coverage of the 2013 London cultured meat tasting event, particularly in the United States, Canada, and the United Kingdom. Using major news outlets, prominent magazines covering food and science issues, and advocacy websites concerning meat consumption, the paper characterizes the overal emphases of the coverage, the tenor of the coverage, and compares the media portrayal of the important issues to the demographic and psychological realities of the actual consumer market into which cultured meat wil compete. In particular, the paper argues that Western media gives a distorted picture of what obstacles are in the path of cultured meat acceptance, especial y by overemphasizing and overrepresenting the importance of the reception of cultured meat among vegetarians. Promoters of cultured meat should recognize the skewed impression that this media coverage provides and pay attention to the demographic data that suggests strict vegetarians are a demographical y negligible group. Resources for promoting cultured meat should focus on the empirical demographics of the consumer market and the empirical psychology of mainstream consumers.

  3. Representation and Dissemination of Intangible Cultural Heritage of Bangladesh through Social Media

    DEFF Research Database (Denmark)

    Khalid, Md. Saifuddin; Chowdhury, Md Saiful Alam

    2016-01-01

    Bangladesh is one of the next eleven countries and home to more than 160 million people. The country is experiencing an exponential growth of social media users due to the increase in affordability of smartphones, literacy rate, education level, and adoption of Internet services and applications...... of strategically representing and diffusing ICH through social media, this research explores the current roles of social media in the transmission of ICH in the virtual world. The research question is: How are Baul song and Jamdani weaving as intangible cultural heritage of Bangladesh represented and disseminated...... through social media platforms?...

  4. 关于批判性媒体或文化研究%Toward a critical media/cultural studies

    Institute of Scientific and Technical Information of China (English)

    道格拉斯·凯尔纳; 吴学琴; 杨婷婷

    2012-01-01

    project was completed by British cultural studies. They thought the conflicts of different interest groups ,such as class, gender, race, ethnicity, and national strata, contributed to the grand view of contemporary media culture,when seen from social production and reproduction. So critical media/cultural studies should have three component parts: media culture production and political economy, media culture texts, and the audience reception and effects of media culture. People can comprehend media culture critically and construct the framework for analyzing media culture only from these perspectives by using diverse cultures and views. This kind of research methods is essentially reading,interpreting, criticizing different human relations occurring in the text , and analyzing the politics of representation formed in media/cultural texts and their mutual exchanges among sense of class, ethnicity and race, gender and sexuality with the background of production, distribution, and consumption and use of social relations. Meanwhile,it also studies how the audience read, understand and use media culture, and how the mainstream culture is produced.

  5. Reengineering the Innovation Culture through Social media Crowdsourcing

    DEFF Research Database (Denmark)

    Scupola, Ada; Nicolajsen, Hanne Westh

    2012-01-01

    innovation culture using theory on organizational culture and crowdsourcing. The analysis shows that the organizational crowdsourcing event has supported an innovation culture change in the case company towards a more including approach to innovation; creating a new and different awareness of innovation...

  6. Foundations of Socio-Cultural Ecology: Consequences for Media Education and Mobile Learning in Schools

    Directory of Open Access Journals (Sweden)

    Klaus Rummler

    2014-07-01

    Full Text Available This conceptual paper offers insights to the foundations of Socio-Cultural Ecology and relates this concept to traditional concepts of Ecology e.g. media ecology or Bronfenbrenner’s ecological model of child development. It will further discuss the term «ecology» as a relation between learners and their surrounding physical and structural world, e. g. an ecology of resources or the classroom as an ecological system. Thirdly more recent concepts in ecology will be considered e. g. Digital Media Ecology including media ecology (German: Medienökologie from a German perspective. This contribution tries to describe common principles of (media ecologies and will ask after their meaning and relation to media education and mobile learning. One of the main results is the realisation that cultural practices of school learning and cultural practices of media acquisition take place in different worlds or in different ecological spheres. The question is thus again of how to bridge these ecological spheres, and how «agency» developed outside school, can be nourished inside school. In other words: how can we bridge socio-cultural and technological structures within these cultural practices.

  7. The Electric Humanities; Patterns for Teaching Mass Media and Popular Culture.

    Science.gov (United States)

    Allen, Don; Warren, Brent

    For generations teachers have tried to teach the approved "classics" of our culture. Today, with the mass media claiming so much of students' time and interest, this approach is more than ever doomed to failure. A better plan is to focus on popular culture: comic books, popular fiction (westerns, horror tales, and science fiction), movies, and…

  8. Culture of non-typeable Haemophilus influenzae from the nasopharynx: Not all media are equal.

    Science.gov (United States)

    Harris, Tegan M; Rumaseb, Angela; Beissbarth, Jemima; Barzi, Federica; Leach, Amanda J; Smith-Vaughan, Heidi C

    2017-03-22

    The efficacy of chocolate agar, versus bacitracin, vancomycin, clindamycin, chocolate agar (BVCCA) for the isolation of non-typeable Haemophilus influenzae (NTHi) from nasopharyngeal swabs was determined. BVCCA cultured NTHi from 97.3% of NTHi-positive swabs, compared to 87.1% for chocolate agar. To maximise culture sensitivity, the use of both media is recommended.

  9. Mediação cultural, informação e ensino

    Directory of Open Access Journals (Sweden)

    Giulia Crippa

    2011-12-01

    Full Text Available O trabalho examina algumas das implicações teóricas e práticas do conceito de mediação cultural e da informação. A partir da descrição e da análise de um evento realizado em 2007 - uma exposição artística, cultural e científica -, são observados potencialidades e desafios das atividades de mediação cultural, com ênfase em seus aspectos formativos e educacionais.

  10. Making Digital Cultures of Gender and Sexuality With Social Media

    Directory of Open Access Journals (Sweden)

    Jean Burgess

    2016-09-01

    Full Text Available This article introduces a special issue concerning the interweaving of gender, sexuality, and social media. There are 10 articles included in the issue which together map out a landscape of diverse areas of interest covering topics such as sexism and harassment, health and wellbeing, relationships, and leisure.

  11. Digital and Media Literacy: Connecting Culture and Classroom

    Science.gov (United States)

    Hobbs, Renee

    2011-01-01

    Today's students tweet, text, and navigate apps up to 12 hours each day, but they may not know how to effectively analyze a TV show or website. Award-winning author Renee Hobbs demonstrates how to incorporate media literacy into the secondary classroom, providing the tools teachers need to: (1) Effectively foster students' critical thinking,…

  12. Effect of different temperature and culture media on the growth of Macrophomina phaseolina.

    Science.gov (United States)

    Csöndes, I; Kadlicskó, S; Gáborjányi, R

    2007-01-01

    The charcoal root disease caused by Macrophomina phaseolina (Tassi) Goidanich may cause considerable damages in hot as well as in dry seasons. The effect of temperature and culture media were studied on the growing patterns of 35 M. phaseolina isolates, collected from different districts of Hungary. The isolates were grown at 10, 15, 20, 25, 30, 35 and 40 degrees C temperatures respectively, and additionally at 25 degrees C on potato-dextrose-, malt-extract-, Czapek-Dox-, Sabouraud-glucose-, maize-flour- and watery agar media, using 90 mm Petri-dishes, 4 repetitions in each case. For all the isolates the most favourable temperature regime was 25 to 35 degrees C and the most advantageous media was the malt-extract-, Sabouraud-glucose- and potato-dextrose-agar media. At these conditions (temperatures and culture media) mycelia growth and the diameter of microsclerotial colonies reached the 90 mm at the 5th day. Mycelia growth of the pathogen was very low at 10, 15 and 40 degrees C, and did not form microsclerotia. On watery agar microsclerotial colony seldom developed, it needed 14 days, and no continuous mycelia developed even in a 8th months culture. Diameter of microsclerotia measured on different culture media varied between 39-308 microm.

  13. Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles.

    Science.gov (United States)

    Veranth, John M; Cutler, N Shane; Kaser, Erin G; Reilly, Christopher A; Yost, Garold S

    2008-03-01

    Cultured lung cells provide an alternative to animal exposures for comparing the effects of different types of air pollution particles. Studies of particulate matter in vitro have reported proinflammatory cytokine signaling in response to many types of environmental particles, but there have been few studies comparing identical treatments in multiple cell types or identical cells with alternative cell culture protocols. We compared soil-derived, diesel, coal fly ash, titanium dioxide, and kaolin particles along with soluble vanadium and lipopolysaccharide, applied to airway-derived cells grown in submerged culture. Cell types included A549, BEAS-2B, RAW 264.7, and primary macrophages. The cell culture models (specific combinations of cell types and culture conditions) were reproducibly different in the cytokine signaling responses to the suite of treatments. Further, Interleukin-6 (IL-6) response to the treatments changed when the same cells, BEAS-2B, were grown in KGM versus LHC-9 media or in media containing bovine serum. The effect of changing media composition was reversible over multiple changes of media type. Other variables tested included culture well size and degree of confluence. The observation that sensitivity of a cell type to environmental agonists can be manipulated by modifying culture conditions suggests a novel approach for studying biochemical mechanisms of particle toxicity.

  14. Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles

    Energy Technology Data Exchange (ETDEWEB)

    Veranth, J.M.; Cutler, N.S.; Kaser, E.G.; Reilly, C.A.; Yost, G.S. [University of Utah, Salt Lake City, UT (United States)

    2008-03-15

    Cultured lung cells provide an alternative to animal exposures for comparing the effects of different types of air pollution particles. Studies of particulate matter in vitro have reported proinflammatory cytokine signaling in response to many types of environmental particles, but there have been few studies comparing identical treatments in multiple cell types or identical cells with alternative cell culture protocols. We compared soil-derived, diesel, coal fly ash, titanium dioxide, and kaolin particles along with soluble vanadium and lipopolysaccharide, applied to airway-derived cells grown in submerged culture. Cell types included A549, BEAS-2B, RAW 264.7, and primary macrophages. The cell culture models (specific combinations of cell types and culture conditions) were reproducibly different in the cytokine signaling responses to the suite of treatments. Further, Interleukin-6 (IL-6) response to the treatments changed when the same cells, BEAS-2B, were grown in KGM versus LHC-9 media or in media containing bovine serum. The effect of changing media composition was reversible over multiple changes of media type. Other variables tested included culture well size and degree of confluence. The observation that sensitivity of a cell type to environmental agonists can be manipulated by modifying culture conditions suggests a novel approach for studying biochemical mechanisms of particle toxicity.

  15. Cultural carrying capacity: Organ donation advocacy, discursive framing, and social media engagement.

    Science.gov (United States)

    Bail, Christopher A

    2016-09-01

    Social media sites such as Facebook have become a powerful tool for public health outreach because they enable advocacy organizations to influence the rapidly increasing number of people who frequent these forums. Yet the very open-ness of social media sites creates fierce competition for public attention. The vast majority of social media messages provoke little or no reaction because of the sheer volume of information that confronts the typical social media user each day. In this article, I present a theory of the "cultural carrying capacity" of social media messaging campaigns. I argue that advocacy organizations inspire more endorsements, comments, and shares by social media users if they diversify the discursive content of their messages. Yet too much diversification creates large, disconnected audiences that lack the sense of shared purpose necessary to sustain an online movement. To evaluate this theory, I created a Facebook application that collects social media posts produced by forty-two organ donation advocacy organizations over 1.5 years, as well as supplemental information about the organization, its audience, and the broader social context in which they interact. Time series models provide strong evidence for my theory net of demographic characteristics of social media users, the resources and tactics of each organization, and broader external factors. I conclude by discussing the implications of these findings for public health, cultural sociology, and the nascent field of computational social science. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Importance of the Mass Media Education: Western Cultural Dilemmas.

    Science.gov (United States)

    Littunen, Yrjo

    1978-01-01

    Discusses features in the Western cultural background which may be relevant to changes in international opinion. Notes problems of freedom of information and cultural processes caused by mass communication among nations, including the high violence content of United States television programs compared with those of Canada and Europe. (MF)

  17. Media Cultures of Young Turkish Migrants and German Resettlers in Germany

    Directory of Open Access Journals (Sweden)

    Annett Heft

    2013-05-01

    Full Text Available This article contributes to the understanding of young people’s media cultures by addressing the question whether and to what extent young people with different cultural backgrounds differ in their exposure to and usage of traditional mass media and new digital media as well as in their engagement in various online activities. It presents empirical data of a German survey about the social environment, media use and Internet behaviour among 605 German resettlers and people with a Turkish migration background aged between 12 and 29 years living in North Rhine-Westphalia and compares the results of the 12- to 19-year old youth with data of the same age group within the German general population. To further assess how cultural and social factors might explain the variation within the youth and young adults with migration background, similarities and differences in their media use patterns are traced with respect to their cultural contexts as well as the factors education, age and gender. The findings are discussed in the context of societal integration of young people with migration background, the homogeneity of mediatised youth cultures and the thesis of the digital divide.

  18. CATEGORICAL IMAGE COMPONENTS IN THE FORMING SYSTEM OF A MARKETING TECHNIQUES MANAGER’S IMAGE CULTURE

    OpenAIRE

    Anna Borisovna Cherednyakova

    2015-01-01

    Based on the understanding of the image culture formation of managers of marketing techniques, as a representative of the social and communication interaction of public structures, categorical apparatus of image culture with an emphasis on the etymology of the image, as an integral component of image culture was analyzed. Categorical components of the image are presented from the standpoint of image culture, as personal new formation, an integral part of the professional activity of the marke...

  19. Cultural text mining: using text mining to map the emergence of transnational reference cultures in public media repositories

    NARCIS (Netherlands)

    Pieters, Toine; Verheul, Jaap

    2014-01-01

    This paper discusses the research project Translantis, which uses innovative technologies for cultural text mining to analyze large repositories of digitized public media, such as newspapers and journals.1 The Translantis research team uses and develops the text mining tool Texcavator, which is base

  20. Analysis of culture media screening data by projection to latent pathways: The case of Pichia pastoris X-33.

    Science.gov (United States)

    Isidro, Inês A; Ferreira, Ana R; Clemente, João J; Cunha, António E; Oliveira, Rui

    2016-01-10

    Cell culture media formulations contain hundreds of individual components in water solutions which have complex interactions with metabolic pathways. The currently used statistical design methods are empirical and very limited to explore such a large design space. In a previous work we developed a computational method called projection to latent pathways (PLP), which was conceived to maximize covariance between envirome and fluxome data under the constraint of metabolic network elementary flux modes (EFM). More specifically, PLP identifies a minimal set of EFMs (i.e., pathways) with the highest possible correlation with envirome and fluxome measurements. In this paper we extend the concept for the analysis of culture media screening data to investigate how culture medium components up-regulate or down-regulate key metabolic pathways. A Pichia pastoris X-33 strain was cultivated in 26 shake flask experiments with variations in trace elements concentrations and basal medium dilution, based on the standard BSM+PTM1 medium. PLP identified 3 EFMs (growth, maintenance and by-product formation) describing 98.8% of the variance in observed fluxes. Furthermore, PLP presented an overall predictive power comparable to that of PLS regression. Our results show iron and manganese at concentrations close to the PTM1 standard inhibit overall metabolic activity, while the main salts concentration (BSM) affected mainly energy expenditures for cellular maintenance.

  1. Cultural Mythology Analysisof Media texts in the Classroom at the Student Audience

    Directory of Open Access Journals (Sweden)

    Alexander Fedorov

    2015-05-01

    Full Text Available The author of this article presents the cultural mythology analysis of media texts: identification and analysis of mythologizing (including in the framework of the so-called folk sources - fairy tales, urban legends, etc. plot, those types of characters, etc. in media texts. In particular, the audience (for example, students offered by critical analysis to answer the question why so many entertainment media texts so popular with a mass audience? The author thinks that the media texts relating to the mass / popular culture, have success with the audience is not due to the fact that they supposedly only target people with low aesthetic taste, subject to psychological pressure, easy to believing the lie, etc., but because their authors respect and learning needs of the audience, including - information, compensatory, hedonistic, recreational, moral, aesthetic, etc.

  2. CATEGORICAL IMAGE COMPONENTS IN THE FORMING SYSTEM OF A MARKETING TECHNIQUES MANAGER’S IMAGE CULTURE

    Directory of Open Access Journals (Sweden)

    Anna Borisovna Cherednyakova

    2015-08-01

    Full Text Available Based on the understanding of the image culture formation of managers of marketing techniques, as a representative of the social and communication interaction of public structures, categorical apparatus of image culture with an emphasis on the etymology of the image, as an integral component of image culture was analyzed. Categorical components of the image are presented from the standpoint of image culture, as personal new formation, an integral part of the professional activity of the marketing techniques manager: object-communicative categorical component, subject-activity categorical component of image, personality-oriented categorical component, value-acmeological categorical component of image.The aim is to identify and justify the image categorical components as a component of image culture of the marketing techniques manager.Method and methodology of work – a general scientific research approach reflecting scientific apparatus of research.Results. Categorical components of the image, as an image culture component of manager of marketing techniques were defined.Practical implication of the results. The theoretical part of «Imageology» course, special course «Image culture of manager of marketing techniques», the theoretical and methodological study and the formation of image culture.

  3. Broader context for social, economic, and cultural components

    Science.gov (United States)

    Patricia L. Winter; Jonathan W. Long; Frank K. Lake; Susan. Charnley

    2014-01-01

    This chapter sets the context for the following sociocultural sections of the synthesis by providing information on the broader social, cultural, and economic patterns in the Sierra Nevada and southern Cascade Range. Demographic influences surrounding population change, including those accounted for through amenity migration, are examined. Social and cultural concerns...

  4. The future of meat: a qualitative analysis of cultured meat media coverage.

    Science.gov (United States)

    Goodwin, J N; Shoulders, C W

    2013-11-01

    This study sought to explore the informational themes and information sources cited by the media to cover stories of cultured meat in both the United States and the European Union. The results indicated that cultured meat news articles in both the United States and the European Union commonly discuss cultured meat in terms of benefits, history, process, time, livestock production problems, and skepticism. Additionally, the information sources commonly cited in the articles included cultured meat researchers, sources from academia, People for the Ethical Treatment of Animals (PETA), New Harvest, Winston Churchill, restaurant owners/chefs, and sources from the opposing countries (e.g. US use some EU sources and vice versa). The implications of this study will allow meat scientists to understand how the media is influencing consumers' perceptions about the topic, and also allow them to strategize how to shape future communication about cultured meat. Published by Elsevier Ltd.

  5. Culturing Fern Gametophytes on Solid Mineral Media for Classroom Study.

    Science.gov (United States)

    Chilton, Glen; Graham, Lane C.

    1988-01-01

    Described are the techniques and results of using this tissue culture activity. Discusses the advantages of using such techniques in teaching alternation of generation life cycles to biology students. (CW)

  6. Viable Legionella Pneumophila Not Detectable by Culture on Agar Media

    Science.gov (United States)

    1987-09-01

    UNIT ELEMENT NO. NO. NO. ACCESSION NO. , N/A 1 1. TITLE (JIncJuue Security Ciaisuicarlon) Viable Legionella Pneumophila not Detectable by Culture on...106 cells-𔄁. Legionella have others. To study this loss of culturability, been sliown to vary in an tigenic composition, virulence L. pneumophila ...COSATICODES 18. SUBJECT TERMS (Continue on reverse if necessary and identify by block number) GROUP SUB-GROUP LEGIONELLA FLUORESCENT ANTIBODY

  7. [Culture media for the detection and the identification of Streptococcus agalactiae].

    Science.gov (United States)

    de la Rosa, M; Pérez, M; Carazo, C; Pareja, L; Orts, A; Cantudo, P

    1994-01-01

    Streptococcus agalactiae, a Group B streptococcus, is the main cause of bacterial perinatal infection and is also an important opportunistic pathogen. Detection and identification of S. agalactiae are straight forward with special culture media, where Group B streptococci show a specific, typical pink or red pigment. To quickly and easily detect the pigment, culture media should contain: (i) starch; (ii) an inhibitor of the folate pathway; (iii) animal serum; (iv) a pepsic proteic hydrolysate; and (v) glucose, together with a high-capacity buffer. When selective antibiotics are added to culture media designed in this way, it is possible to detect S. agalactiae directly from clinical samples by observation of its pigment after less than 12 hours of aerobic incubation.

  8. A mediação cultural como categoria autônoma.

    Directory of Open Access Journals (Sweden)

    Edmir Perrotti

    2014-10-01

    Full Text Available Introdução: Reflexão sobre a noção de mediação cultural,  como categoria teórica e operacional autônoma, definida em articulação permanente com as  da produção e da recepção culturais, considerados processos dinâmicos e complexos que regem a ecologia simbólica.Objetivo: definir a mediação cultural como instância essencial dos processos de produção de sentidoMetodologia: estudo dos elementos constitutivos de uma experiência cultural autobiográfica,  relatada por Clarice Lispector no conto Felicidade Clandestina.Resultados: a mediação cultural não é simples recurso de transferência de dados/informações, mero “canal” ou instância de apoio visando a criação de elos entre sujeitos.Conclusão: A mediação cultural  é ato autônomo, com identidade e lógicas próprias, definidas em relação com as esferas da produção e da recepção de informação e cultura. Tal abordagem, assumindo modelo triádico (mediação-produção-recepção, rompe com compreensões dualistas e mecânicas dos campos da Informação e da Comunicação, mostrando-se heurística, posto que se compatível com a centralidade dos dispostivos de mediação cultural na atualidade.

  9. Microspectroscopic investigation of the membrane clogging during the sterile filtration of the growth media for mammalian cell culture.

    Science.gov (United States)

    Cao, Xiaolin; Loussaert, James A; Wen, Zai-qing

    2016-02-01

    Growth media for mammalian cell culture are very complex mixtures of several dozens of ingredients, and thus the preparation of qualified media is critical to viable cell density and final product titers. For liquid media prepared from powdered ingredients, sterile filtration is required prior to use to safeguard the cell culture process. Recently one batch of our prepared media failed to pass through the sterile filtration due to the membrane clogging. In this study, we report the root cause analysis of the failed sterile filtration based on the investigations of both the fouling media and the clogged membranes with multiple microspectroscopic techniques. Cellular particles or fragments were identified in the fouling media and on the surfaces of the clogged membranes, which were presumably introduced to the media from the bacterial contamination. This study demonstrated that microspectroscopic techniques may be used to rapidly identify both microbial particles and inorganic precipitates in the cell culture media.

  10. Ethno-cultural component in Sochi residents’ geopolitical consciousness

    Directory of Open Access Journals (Sweden)

    Svetlana V. Petrova

    2011-04-01

    Full Text Available The article concerns crucial issues of Sochi residents’ political consciousness features formation in the context of toleration and interethnic dialog development, analyses factors, having effect on cultures interpenetration in Sochi politics.

  11. Chinese Traditional Cultural Components in The Joy Luck Club

    Institute of Scientific and Technical Information of China (English)

    黄秀琴

    2010-01-01

    In the film The Joy Luck Club, which is written originally by a Chinese-American writer, Amy Tan, describes the relation-ship between the Joy Luck Club mothers and their daughters and their cultural conflicts. There are four pairs of mother-daughter. Mothers are the first generation immigrants who come from the Chinese traditional families with Chinese traditional culture in their minds firmly in 1940s while their daughters are born and raised in American, the second generations immigrants, who don't quiet understand their mot-hers' Chinese traditional culture and the way their thinking. So there are misunderstandings and conflicts between them, but finally the daughters gradually understand their mothers and their Chinese traditional culture through the great efforts the mothers make.

  12. Impact of different culture media on hairy roots growth of Valeriana officinalis L.

    OpenAIRE

    Pakdin Parizi, Ali; Farsi, Mohammad; Nematzadeh, Ghorban-Ali; Amin MIRSHAMSI

    2015-01-01

    Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V. officinalis hairy roots, MS, B5 media (1.0X and 0.5X strength), N6 medium and a modified MS medium without phytohormones were used. In addition, different NH4+ to NO3- ratios in MS medium were studied. The effects of these treatments were evaluated after 21 days of culture in relation to hairy roo...

  13. Two-component vector solitons in defocusing Kerr-type media with spatially modulated nonlinearity

    Energy Technology Data Exchange (ETDEWEB)

    Zhong, Wei-Ping, E-mail: zhongwp6@126.com [Department of Electronic and Information Engineering, Shunde Polytechnic, Guangdong Province, Shunde 528300 (China); Texas A and M University at Qatar, P.O. Box 23874 Doha (Qatar); Belić, Milivoj [Texas A and M University at Qatar, P.O. Box 23874 Doha (Qatar); Institute of Physics, University of Belgrade, P.O. Box 57, 11001 Belgrade (Serbia)

    2014-12-15

    We present a class of exact solutions to the coupled (2+1)-dimensional nonlinear Schrödinger equation with spatially modulated nonlinearity and a special external potential, which describe the evolution of two-component vector solitons in defocusing Kerr-type media. We find a robust soliton solution, constructed with the help of Whittaker functions. For specific choices of the topological charge, the radial mode number and the modulation depth, the solitons may exist in various forms, such as the half-moon, necklace-ring, and sawtooth vortex-ring patterns. Our results show that the profile of such solitons can be effectively controlled by the topological charge, the radial mode number, and the modulation depth. - Highlights: • Two-component vector soliton clusters in defocusing Kerr-type media are reported. • These soliton clusters are constructed with the help of Whittaker functions. • The half-moon, necklace-ring and vortex-ring patterns are found. • The profile of these solitons can be effectively controlled by three soliton parameters.

  14. Media and cultural influences in african-american girls' eating disorder risk.

    Science.gov (United States)

    Jones, Lakaii A; Cook-Cottone, Catherine

    2013-01-01

    Objective. To investigate media and cultural influences in eating disorder development in African-American adolescent females. Method. Fifty-seven participants were recruited through churches and community organizations to complete a questionnaire. Results. Mainstream sociocultural identification was associated with more eating disorder behavior in African-American females; cultural ethnic identification was not significantly associated with eating disorder behavior in African-American females, mainstream sociocultural identification, cultural ethnic identification, and body dissatisfaction significantly predicted eating disorder behavior; and cultural ethnic identification was positively correlated with mainstream sociocultural identification. This study provides support for the importance of eating disorder prevention interventions that focus specifically on African-American girls.

  15. Youth culture, media and sexuality: What could faith communities ...

    African Journals Online (AJOL)

    2012-02-14

    Feb 14, 2012 ... space in which a theological understanding of sex and sexuality is facilitated ... in relation to how the social category of 'youth' and their way of living, ... Schools became one of the most important societal structures where youth culture developed ..... This interaction and acknowledgement of one another as.

  16. In vitro culture of individual mouse preimplantation embryos: the role of embryo density, microwells, oxygen, timing and conditioned media.

    Science.gov (United States)

    Kelley, Rebecca L; Gardner, David K

    2017-02-15

    Single embryo culture is suboptimal compared with group culture, but necessary for embryo monitoring, and culture systems should be improved for single embryos. Pronucleate mouse embryos were used to assess the effect of culture conditions on single embryo development. Single culture either before or after compaction reduced cell numbers (112.2 ± 3.1; 110.2 ± 3.5) compared with group culture throughout (127.0 ± 3.4; P media volume from 20 µl to 2 µl increased blastocyst cell numbers in single embryos cultured in 5% oxygen (84.4 ± 3.2 versus 97.8 ± 2.8; P media to single embryos increased hatching rate and blastocyst cell number (91.5 ± 4.7 versus 113.1 ± 4.4; P media volume and microwells influence single embryo development; and embryo-conditioned media may substitute for group culture.

  17. Culture and spirituality: essential components of palliative care.

    Science.gov (United States)

    Speck, Peter

    2016-06-01

    Palliative care advocates a holistic, multiprofessional approach to the care of people with life-threatening disease. In addition to the control of physical symptoms attention should also be paid to psychosocial, cultural and spiritual aspects of the patient's experience of illness. Guidance documents and research evidence reflect the complexity of the patient's journey and the need to regularly assess these areas of need over time. Cultural background can shape how patients respond to life-threatening illness, as can the beliefs held by the patients, whether religious or more broadly spiritual. Research evidence shows the importance of identifying and addressing cultural and spiritual aspects of care held by patients, families and staff. These are often neglected in clinical practice due to the focus on biomedical concerns and staff discomfort in engaging with beliefs and culture. Recent studies have highlighted gaps in the research, and some methodological difficulties and indicate many patients welcome healthcare staff enquiring about the importance of their beliefs and culture. Identifying research priorities is necessary to guide future research and strengthen the evidence base.

  18. The Conceptualisation of the Cultural Component of Language Teaching in Australian Language-in-Education Policy

    Science.gov (United States)

    Liddicoat, Anthony J.

    2004-01-01

    Culture is recognised as an important part of languages teaching in Australia and has been increasingly integrated into policy and curriculum documents and the general rhetoric of languages education. The result is that policies include statements about aspects of cultural competence. However, the nature and scope of the cultural component in…

  19. Media and Cultural Influences in African-American Girls’ Eating Disorder Risk

    OpenAIRE

    Jones, Lakaii A.; Cook-Cottone, Catherine

    2013-01-01

    Objective. To investigate media and cultural influences in eating disorder development in African-American adolescent females. Method. Fifty-seven participants were recruited through churches and community organizations to complete a questionnaire. Results. Mainstream sociocultural identification was associated with more eating disorder behavior in African-American females; cultural ethnic identification was not significantly associated with eating disorder behavior in African-American female...

  20. Mass media tests: socio-cultural aspect (based on advertising texts)

    OpenAIRE

    MOSHCHEVA SVETLANA

    2014-01-01

    The article is devoted to the social and cultural aspects of mass media texts. The ways and the principles of this research are defined. The study concludes that the social nature of advertising communication enables it to reflect all the nuances of life of society and to form an expedient paradigm of human relations in the context of various social and cultural phenomena of reality.

  1. Cultural Reductionism and the Media: Polarising Discourses around Schools, Violence and Masculinity in an Age of Terror

    Science.gov (United States)

    Mills, Martin; Keddie, Amanda

    2010-01-01

    This paper provides a media analysis of three interrelated sets of newspaper articles dealing with youth, schooling and violence. Understanding the media as a dominant and powerful cultural text that creates the realities it describes, the paper takes a critical view of the 'standpoint' of recent media representations of the Cronulla (Sydney,…

  2. Expression of microRNAs in bovine and human pre-implantation embryo culture media

    Directory of Open Access Journals (Sweden)

    Jenna eKropp

    2014-04-01

    Full Text Available MicroRNAs (miRNA are short non-coding RNAs which act to regulate expression of genes driving numerous cellular processes. These RNAs are secreted within exosomes from cells into the extracellular environment where they may act as signaling molecules. In addition, they are relatively stable and are specifically expressed in association to certain cancers making them strong candidates as biological markers. Moreover, miRNAs have been detected in body fluids including urine, milk, saliva, semen, and blood plasma. However, it is unknown whether they are secreted by embryonic cells into the culture media. Given that miRNAs are expressed throughout embryonic cellular divisions and embryonic genome activation, we hypothesized that they are secreted from the embryo into the extracellular environment and may play a role in the developmental competence of bovine embryos. To test this hypothesis, bovine embryos were cultured individually from day 5 to day 8 of development in an in vitro fertilization system and gene expression of 5 miRNAs was analyzed in both embryos and culture media. Differential miRNA gene expression was observed between embryos that developed to the blastocyst stage and those that failed to develop from the morula to blastocyst stage, deemed degenerate embryos. MiR-25, mir-302c, miR-196a2, and miR-181a expression was found to be higher in degenerate embryos compared to blastocyst embryos. Interestingly, these miRNAs were also found to be expressed in the culture media of both bovine and human pre-implantation embryos. Overall, our results show for the first time that miRNAs are secreted from pre-implantation embryos into culture media and that miRNA expression may correlate with developmental competence of the embryo. Expression of miRNAs in in vitro culture media could allow for the development of biological markers for selection of better quality embryos and for subsequent successful pregnancy.

  3. Navigating Online Selves: Social, Cultural, and Material Contexts of Social Media Use by Diasporic Gay Men

    Directory of Open Access Journals (Sweden)

    Alexander Dhoest

    2016-09-01

    Full Text Available Social media not only create new opportunities but also pose new challenges for the ways people navigate their online selves. As noted by boyd, social media are characterized by unique dynamics such as collapsed contexts, implying that one’s distinct offline social worlds meet online. This creates particular challenges for lesbian, gay, bisexual, transgender, and queer (LGBTQ people, at least those who find it crucial to maintain distinct contexts in which they disclose or conceal their gender and/or sexual selves. However, the existing scholarship on social media use by LGBTQs is predominantly anchored in English-language Western contexts and tends to lose sight of the cultural specificities of Internet use. Therefore, in this article, we build on the scholarship to further investigate the role of context for disclosing or concealing gender and/or sexual selves online. More specifically, we ask, “How do social, cultural, and material contexts affect the ways LGBTQs navigate their selves on social media?” To investigate this question, we analyze in-depth face-to-face interviews with gay men who themselves, or whose parents, migrated to Belgium. Because their migration background forces them to negotiate different social, cultural, and material contexts, our focus on diasporic gay men helps to bring out the issue of context in social media use.

  4. Production of bacterial cellulose using different carbon sources and culture media.

    Science.gov (United States)

    Mohammadkazemi, Faranak; Azin, Mehrdad; Ashori, Alireza

    2015-03-01

    In this work, the effects of carbon sources and culture media on the production and structural properties of bacterial cellulose (BC) have been studied. BC nanofibers were synthesized using Gluconacetobacter xylinus strain PTCC 1734. Media used were Hestrin-Schramm (H), Yamanaka (Y), and Zhou (Z). Five different carbon sources, namely date syrup, glucose, mannitol, sucrose, and food-grade sucrose were used in these media. All the produced BC pellicles were characterized in terms of dry weight production, biomass yield, thermal stability, crystallinity and morphology by thermogravimetric analysis (TGA), x-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM). The obtained results showed that mannitol lead to the highest yield, followed by sucrose. The highest production efficiency of mannitol might be due to the nitrogen source, which plays an important role. The maximum improvement on the thermal stability of the composites was achieved when mannitol was used in H medium. In addition, the crystallinity was higher in BC formed in H medium compared to other media. FE-SEM micrographs illustrated that the BC pellicles, synthesized in the culture media H and Z, were stable, unlike those in medium Y that were unstable. The micrographs of BC produced in media containing mannitol and sucrose provided evidence of the strong interfacial adhesion between the BC fibers without noticeable aggregates.

  5. Advertising as a socio-cultural field: discursive component

    Directory of Open Access Journals (Sweden)

    O. I. Gerus

    2016-10-01

    Full Text Available Using the theory of social fields by P. Bourdieu the article describes the features of the advertising as a symbolic cultural field functioning. Confined to the locality of its actions, advertising creates cultural forms, images, facilities, value­based judgments that become widespread, claiming the role of human behavior that must be followed. The author defines advertising as a symbolic cultural field. To analyse the advertising field and determine the impact of advertising on learning and the formation of certain values and cultural patterns the author justifies application of critical discourse analysis (CDA. The meaning of notions «advertising discourse» and «discourses of advertising» are being exposed and delineated. The author propose and test research methodology of development and impact of advertising discourse on advertising consumers. The types of value discourses prevalent in the field of commercial advertising were identified. In the study, the author found that advertising discourses are dynamic entities that are changed according to time and the state of the society in which they operate. In particular, the discourses produced in advertising influences social context ­ developments in society, the dominant values and attitudes.

  6. Nutrient and media recycling in heterotrophic microalgae cultures.

    Science.gov (United States)

    Lowrey, Joshua; Armenta, Roberto E; Brooks, Marianne S

    2016-02-01

    In order for microalgae-based processes to reach commercial production for biofuels and high-value products such as omega-3 fatty acids, it is necessary that economic feasibility be demonstrated at the industrial scale. Therefore, process optimization is critical to ensure that the maximum yield can be achieved from the most efficient use of resources. This is particularly true for processes involving heterotrophic microalgae, which have not been studied as extensively as phototrophic microalgae. An area that has received significant conceptual praise, but little experimental validation, is that of nutrient recycling, where the waste materials from prior cultures and post-lipid extraction are reused for secondary fermentations. While the concept is very simple and could result in significant economic and environmental benefits, there are some underlying challenges that must be overcome before adoption of nutrient recycling is viable at commercial scale. Even more, adapting nutrient recycling for optimized heterotrophic cultures presents some added challenges that must be identified and addressed that have been largely unexplored to date. These challenges center on carbon and nitrogen recycling and the implications of using waste materials in conjunction with virgin nutrients for secondary cultures. The aim of this review is to provide a foundation for further understanding of nutrient recycling for microalgae cultivation. As such, we outline the current state of technology and practical challenges associated with nutrient recycling for heterotrophic microalgae on an industrial scale and give recommendations for future work.

  7. Social Media in the Science Classroom: Using Instagram With Young Women to Incorporate Visual Literacy and Youth Culture

    Science.gov (United States)

    Serpagli, Lauren Paola

    The purpose of this study is to explore the impact that a digital, picture sharing platform, specifically Instagram, can have on the learning experience in the biology classroom. Students are surrounded by a societal culture inundated with technology, including smart phones and social media, and science educators need to find ways to harness the popularity of these tools in the classroom. The theoretical frameworks guiding this study are Culturally Relevant Pedagogy (CRP), Digital Visual Literacy, and a Critical Feminism. To understand the many ways of social media, specifically Instagram, could influence science content understanding in the classroom, the research methodology used was a connective ethnography. This approach allowed for analysis for the creation of the dual-setting of the classroom and the digital platform and the emerging culture that resulted. As Instagram was used as the virtual component of the classroom, this gave rise to a new identity for the classroom, one in which a digital culture was established. Instagram served as an extension of the classroom space that was not limited by time, location, or teacher availability. The participants in this study were female high school biology students in New York City. An Instagram profile was created for the course and used in different ways: To post homework reminders, lab pictures, biology memes, current events, and discoveries, thereby exposing students to science in "nontraditional" ways. Students discussed their reactions and feelings of the uses and effectiveness of Instagram in the class and made suggestions for future applications through questionnaires, focus groups, and individual interviews. Findings reveal Instagram to ease access for review and reminders, integrate teenage culture into learning, and serve as an effective supplement tool to traditional classroom instruction. One chief goal of this research project was to help educators increase their understanding of the role that social

  8. Local Political Culture and Use of Local Media: Is There a Relationship?

    DEFF Research Database (Denmark)

    Hoff, Jens Villiam

    this model also turned out to be quite apt in explaining variation in local media use, the citizen role model was at least as good as this model in explaining these variations. Thus, our model of the four citizen roles/four different local cultures seems to be very robust when it comes to explaining......This paper investigates whether there is a relationship between local political culture and use of local media; especially the internet. The investigation is done on the basis of a case study conducted in the Danish municipality of Odder between 2003 and 2006. The case study is a very thorough...... investigation of local media, local politics and local democracy. In connection with the case study a wealth of qualitative as well as quantitative data were collected, among these two surveys conducted in 2003 and 2005. On the basis of a triangulation between theoretical considerations concerning...

  9. Social media, digital video and health promotion in a culturally and linguistically diverse Australia.

    Science.gov (United States)

    O'Mara, Ben

    2013-09-01

    Participatory processes are effective for digital video production that promotes health and wellbeing with communities from diverse cultural and linguistic backgrounds, including migrants and refugees. Social media platforms YouTube, Vimeo, Flickr and others demonstrate potential for extending and enhancing this production approach. However, differences within and between communities in terms of their quality of participation online suggest that social media risk becoming exclusive online environments and a barrier to health and wellbeing promotion. This article examines the literature and recent research and practice in Australia to identify opportunities and challenges when using social media with communities from diverse cultural and linguistic backgrounds. It proposes a hybrid approach for digital video production that integrates 'online' and 'offline' participation and engages with the differences between migrants and refugees to support more inclusive health and wellbeing promotion using digital technology.

  10. USES OF SOCIAL MEDIA TO PROMOTE ASEAN SOCIO-CULTURAL COMMUNITY IN VIETNAM

    Directory of Open Access Journals (Sweden)

    Ulas Basar Gezgin

    2017-01-01

    Full Text Available The global openness of Vietnam brought out very fast major social changes. The country is a party to a number of international economic agreements and frameworks including World Trade Organization, Trans Pacific Partnership as well as ASEAN (Association of South East Asian Nations. External economic factors such as those associated with South East Asian integration are coupled with ASEAN’s ambition to set up a socio-cultural community in addition to the economic community. In the meantime, the popularity of social media is rapidly growing in Vietnam with more than 30 million Vietnamese Facebook accounts. Considering these 2 major influences, ie social media and regional integration efforts, this article proposes a number of recommendations to use social media to promote ASEAN Socio-Cultural Community.

  11. Culturing Chaetoceros muelleri using simplified media with different N sources: effects on production and lipid content

    NARCIS (Netherlands)

    Reis Batista, Isabel; Garcia, Ainhoa Blanco; Dalen, Van Pim; Kamermans, Pauline; Verdegem, Marc; Smaal, Aad C.

    2015-01-01

    Land-based bivalve aquaculture depends on large-scale cultures of live microalgae for food. The intensity of large-scale microalgal production is important for cost-effectiveness. Using Walne’s medium as the control, simplified media containing nitrogen, phosphorus, silica, iron, manganese and

  12. Impact of different culture media on hairy roots growth of Valeriana officinalis L.

    Directory of Open Access Journals (Sweden)

    Ali PAKDIN PARIZI

    2015-12-01

    Full Text Available Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V. officinalis hairy roots, MS, B5 media (1.0X and 0.5X strength, N6 medium and a modified MS medium without phytohormones were used. In addition, different NH4+ to NO3- ratios in MS medium were studied. The effects of these treatments were evaluated after 21 days of culture in relation to hairy root growth. B5 and ½ B5 media were the best basal media for hairy root growth. MS medium supplemented with a 20:20 ratio (mM of NH4+ to NO3- displayed highest growth rates and biomass yield in hairy root cultures. The present study demonstrated that the composition of culture medium and the ratio of different nitrogen sources have significant impact on the growth of V. officinalis hairy roots.

  13. Media, Tourism, Environment, and Cultural Issues in Australia: A Case Study of a Study Abroad Program

    Science.gov (United States)

    Freedman, Eric

    2010-01-01

    A multidisciplinary study abroad program developed by a U.S. journalism school and cosponsored by a college of agriculture and natural resources interweaves the themes of mass media, tourism, environment, and cultural issues in Australia. This article traces the development and evolution of the faculty-led program and discusses its curriculum,…

  14. Burmese Attitude toward Chinese: Portrayal of the Chinese in Contemporary Cultural and Media Works

    National Research Council Canada - National Science Library

    Min Zin

    2012-01-01

    .... Such sentiment is not just transient public opinion, but an attitude. The author measures it by studying contemporary cultural and media works as found in legally published expressions, so as to exclude any material rejected by the regime’s censors...

  15. Selection of chemically defined media for CHO cell fed-batch culture processes

    NARCIS (Netherlands)

    Pan, X.; Streefland, M.; Dalm, C.; Wijffels, R.H.; Martens, D.E.

    2017-01-01

    Two CHO cell clones derived from the same parental CHOBC cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures.
    Higher a

  16. Bullying the media : Cultural and climato-economic readings of press repression versus press freedom

    NARCIS (Netherlands)

    Van de Vliert, E.

    Journalists and media assistants in many places are murdered, imprisoned, censored, threatened, and similarly harrassed. Here I document that, and explain why, there are three climato-economic niches of press repression versus press freedom as part of broader syndromes of national culture. A

  17. Social Critique "and" Pleasure: Critical Media Literacy with Popular Culture Texts

    Science.gov (United States)

    Gainer, Jesse

    2007-01-01

    This article addresses the importance of opening space for young people to engage in critical media literacy learning using popular culture texts. Children's background knowledge includes a wide-variety of texts that are often ignored or excluded from school curriculum. The author shows how popular music, for example, can offer powerful…

  18. Either/or Rules: Social Studies Teachers' Talk about Media and Popular Culture

    Science.gov (United States)

    Mangram, Jefery A.

    2008-01-01

    This article examines how 15 secondary social studies teachers made meaning of media and popular culture, and how those perspectives informed their relationships with their students. Using data from a 3-year qualitative study in which multiple in-depth interviews were conducted, this article also analyzes the discourses that circulated in the…

  19. Remote Control Childhood: Combating the Hazards of Media Culture in Schools

    Science.gov (United States)

    Levin, Diane

    2010-01-01

    Background: Media culture touches most aspects of the lives of children growing up today, beginning at the earliest ages. It is profoundly the lessons children learn as well as how they learn, thereby contributing to what this article characterizes as "remote control childhood." Educators need to understand remote control childhood so…

  20. Homocysteine in embryo culture media as a predictor of pregnancy outcome in assisted reproductive technology.

    Science.gov (United States)

    Boyama, Burcu Aydin; Cepni, Ismail; Imamoglu, Metehan; Oncul, Mahmut; Tuten, Abdullah; Yuksel, Mehmet Aytac; Kervancioglu, Mehmet Ertan; Kaleli, Semih; Ocal, Pelin

    2016-01-01

    The aim of this study was to determine whether homocysteine (hcy) concentrations in embryo culture media correlate with pregnancy outcome in assisted reproductive technology (ART) cycles. Forty patients who underwent single embryo transfer at the infertility clinic of a tertiary care center were recruited for this case-control study. Spent embryo culture media from all patients were collected after single embryo transfer on day 3 (n = 40). Hcy concentrations in embryo culture media were analyzed by enzyme cycling method. Patients were grouped according to the diagnosis of a clinical pregnancy. Sixteen patients were pregnant while 24 patients failed to achieve conception. Mean Hcy levels in the culture media were significantly different between the groups (p pregnant group and 3.37 ± 0.92 μmol/l in the pregnant group. Receiver operator curve analysis for determining the diagnostic potential of Hcy for pregnancy revealed an area under the curve of 0.792 (confidence interval: 0.65-0.94; p < 0.05). A cut-off value of 3.53 μmol/l was determined with a sensitivity of 83.3%, and a specificity of 68.8%. Lower hcy levels were associated with a better chance of pregnancy and better embryo grades. Hcy may be introduced as an individual metabolomic profiling marker for embryos.

  1. Media, Tourism, Environment, and Cultural Issues in Australia: A Case Study of a Study Abroad Program

    Science.gov (United States)

    Freedman, Eric

    2010-01-01

    A multidisciplinary study abroad program developed by a U.S. journalism school and cosponsored by a college of agriculture and natural resources interweaves the themes of mass media, tourism, environment, and cultural issues in Australia. This article traces the development and evolution of the faculty-led program and discusses its curriculum,…

  2. [Automated methods of culture determination of M. tuberculosis in liquid media].

    Science.gov (United States)

    Irtuganova, O A; Smirnova, N S; Slogotskaia, L V; Moroz, A M; Litvinov, V I

    2001-01-01

    A hundred and seventy respiratory samples from patients with different forms of tuberculosis were used to test the efficiency of the automatic liquid culture systems BACTEC MGIT 960 and MB/BacT with inoculation into the standard dense media. All these media provided 47 M. tuberculous isolates, of them 41 (87.2%), 38 (80.9%), and 76.6% on the BACTER 960, MB/BacT, and dense media, respectively. The average time of detection of mycobacterial growth by means of automatic systems was much shorter and equal to 10.7 days on the BACTEC 960 and 18.7 days on the MB/BacT versus 33.2 days on the standard dense medium. In terms of their sensitivity and detection rate, the automatic systems were superior to the dense media widely used in laboratory practice.

  3. Social media in higher education: A look at participatory culture in graduate coursework

    Directory of Open Access Journals (Sweden)

    Ann-Louise Davidson

    2013-01-01

    Full Text Available Society has become fascinated with web- based social media. Recently, aspects of social media environments such as participatory culture, new media digital literacies, and connectivism have been increasingly investigated. However, current university policies often restrict, if not forbid, the use of social networking sites in class. For professors seeking to introduce social media into their teaching practice, these restrictive policies can make it difficult to teach with and about social computing and computer-supported collaborative work. This descriptive paper presents the experiences of two professors who integrated Web 2.0 practices into their respective graduate-level education courses titled Social Computing and Computer-Supported Collaborative Work and Web 2.0 = Pedagogy 2.0? and describes their underlying theories and concepts. Subsequently, the courses’ rationales theoretical underpinnings, and teaching approaches are delineated, and implementation strategies are suggested.

  4. Identification of suitable media based on hydroponic culture for production Zucchini squash

    Directory of Open Access Journals (Sweden)

    TP Suvo

    2016-12-01

    Full Text Available An experiment was conducted to identify the hydroponic culture based suitable media for the production of Zucchini Squash in the Biochemistry Laboratory, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh during 2014. Zucchini plant (Cucurbita pepo L. were grown in closed soilless systems to determine the effect of four different hydroponics media on plant growth, yield and nutrient contents (fruit moisture content, ascorbic acid content on fruit, fruit protein content, protein content in leaves. Three types of substrates (coconut husk, jute, cotton along with Hoagland solution were used in this experiment. Result revealed that media using Jute fiber showed significant effect on plant growth and nutritional values than the other media (media of cotton with Hoagland solution, coconut husk with Hoagland solution and only Hoagland solution. The plant grown using jute media showed the highest plant height (60.33 cm, number of leaves (17.33, yield (1.5 kg plant-1, fruit moisture content (97.33%, Ascorbic acid content in fruit (28.73 mg 100g-1, protein percentage in fruit (1.406% and percentage (1.326% in leaves than the other media. Therefore, with the controlled nutrient supply, less expense, less labor, no use of pesticides or fertilizer with controlled environment the use of jute fiber as a substrate with Hoagland solution can be an effective one.

  5. Setting the agenda: Different strategies of a Mass Media in a model of cultural dissemination

    Science.gov (United States)

    Pinto, Sebastián; Balenzuela, Pablo; Dorso, Claudio O.

    2016-09-01

    Day by day, people exchange opinions about news with relatives, friends, and coworkers. In most cases, they get informed about a given issue by reading newspapers, listening to the radio, or watching TV, i.e., through a Mass Media (MM). However, the importance of a given new can be stimulated by the Media by assigning newspaper's pages or time in TV programs. In this sense, we say that the Media has the power to "set the agenda", i.e., it decides which new is important and which is not. On the other hand, the Media can know people's concerns through, for instance, websites or blogs where they express their opinions, and then it can use this information in order to be more appealing to an increasing number of people. In this work, we study different scenarios in an agent-based model of cultural dissemination, in which a given Mass Media has a specific purpose: To set a particular topic of discussion and impose its point of view to as many social agents as it can. We model this by making the Media has a fixed feature, representing its point of view in the topic of discussion, while it tries to attract new consumers, by taking advantage of feedback mechanisms, represented by adaptive features. We explore different strategies that the Media can adopt in order to increase the affinity with potential consumers and then the probability to be successful in imposing this particular topic.

  6. Different culture media containing methyldopa for melanin production by Cryptococcus species

    Directory of Open Access Journals (Sweden)

    Ralciane de Paula Menezes

    2011-10-01

    Full Text Available INTRODUCTION: Melanin production by species of Cryptococcus is widely used to characterize C. neoformans complex in mycology laboratories. This study aims to test the efficacy of methyldopa from pharmaceutical tablet as a substrate for melanin production, to compare the production of melanin using different agar base added with methyldopa, and to compare the melanin produced in those media with that produced in Niger seed agar and sunflower seed agar by C. neoformans, C. laurentii, and C. albidus. Two isolates of each species, C. neoformans, C. laurentii, and C. albidus, and one of Candida albicans were used to experimentally detect conditions for melanin production. METHODS: The following media were tested: Mueller-Hinton agar (MHA, brain and heart infusion agar (BHIA, blood agar base (BAB, and minimal medium agar (MMA, all added with methyldopa, and the media Niger seed agar (NSA and sunflower seed agar (SSA. RESULTS: All isolates grew in most of the culture media after 24h. Strains planted on media BAB and BHIA showed growth only after 48h. All isolates produced melanin in MMA, MHA, SSA, and NSA media. CONCLUSIONS: Methyldopa in the form pharmaceutical tablet can be used as a substrate for melanin production by Cryptococcus species; minimal medium plus methyldopa was more efficient than the BAB, MHA, and BHIA in the melanin production; and NSA and SSA, followed by MMA added with methyldopa, were more efficient than other media studied for melanin production by all strains studied.

  7. Cultural self-awareness as a crucial component of military cross-cultural competence.

    Science.gov (United States)

    Pappamihiel, Constantine J; Pappamihiel, Eleni

    2013-01-01

    The military forces in the United States represent a unique culture that includes many subcultures within their own military society. Acculturation into the military often deemphasizes the influence of personal narrative and thereby establishes the primacy of military culture over personal cultural influences. The authors make the argument that military personnel need to further develop an understanding and appreciation of personal cultural narrative as well as organizational culture. The increased integration of military personnel with interagency partners, along with cooperative efforts between relief organizations, and nongovernmental organizations in politically/economically unstable areas around the globe serves to make cross-cultural interaction unavoidable in the future. Military medical personnel are especially likely to interact with others who have culturally different values. These interactions can occur between organizations as easily as they can during patient care. They must be able to step outside of their military culture and develop cross-cultural competence that is grounded in cultural self-awareness. Without an appropriate level of cultural self-awareness, military and medical personnel run the risk of being unable to communicate across dissimilar cultures or worse, alienating key stakeholders in collaborative operations between military services, coalition partners, and nonmilitary organizations. It is the authors? contention that unless military personnel, especially those in the medical arena, are able to appropriately self-assess situations that are impacted by culture, both their own and the other personnel involved, the resulting cultural dissonance is more likely to derail any significant positive effect of such collaborations.

  8. CROSSING THE THRESHOLD OF HOPE INTO THE MEDIA CULTURE

    Directory of Open Access Journals (Sweden)

    Margaret J. Obrovac

    2015-09-01

    Full Text Available The “new atheism” and the “new evangelization” have become the buzzwords of the age. Atheism is now the fastest growing “religious” group in the United States; the new evangelization decisively shaped the conclave that elected Jorge Bergoglio to the papacy. Twenty years ago, in Crossing the Threshold of Hope, John Paul II reflected pastorally on some of the philosophical, spiritual, and cultural roots of both. His insights, embodied in Christians who live them, offer the Church a key to our times. If evangelization today is to announce the Gospel in the languages of today, what script might it use? What images might it evoke? What might its cadence be like?

  9. Battling for Shared Culture Between Indonesia and Malaysia in The Social Media Era

    Directory of Open Access Journals (Sweden)

    Liliek Adelina Suhardjono

    2012-04-01

    Full Text Available This is a research paper that takes as its central issue recent contestations and negotiations between Indonesia and Malaysia regarding certain forms of these countries’ intangible cultural heritage. In recent years, there has been an increasing incident in relation with cultural conflicts that happens between these two neighboring countries. These cultural conflicts specifically referred to the traditional performance arts, which has been hereditarily bequeathed by their respective ancestors. Despite the facts that both countries shared the same cultural roots, each side seems has a tendency to despise each other whenever the subject of culture ownership is being brought: both claim for the same cultural rights. Questions on which side is more entitled to the rights became public fierce debates; and it wildly happened especially in the online world through the medium of social networking platform. This paper examines the phenomenon on how should the ownership of authenticity be placed. And since it happens in the modern societies which cannot be separated from their digital personas, the role of social media is inexorably also be addressed. It will link the two identities of the public who is initially mere the audience of the performance then transform into the owner of the cultural product in dispute. This essay begins by a major length discussion about the concept of authenticity in culture, along with the ownership of intangible heritage, the motivation for claiming authenticity, and the protection of cultural property. It will then go on to the topic of the major changes brought by social media in the modern society. Subsequently it will continue to the state of cultural conflict between Indonesia and Malaysia over ‘stolen’ cultural heritage. 

  10. Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free or chemically defined media.

    Science.gov (United States)

    Serrato, J Antonio; Hernández, Vanessa; Estrada-Mondaca, Sandino; Palomares, Laura A; Ramírez, Octavio T

    2007-06-01

    SFM (serum-free medium) is preferred to media containing animal-derived components when culturing mammalian cells for the production of therapeutic recombinant proteins and mAbs (monoclonal antibodies). Nonetheless, eliminating animal-derived components from media can strongly modify culture performance and alter protein glycosylation. In the present study, mAb glycosylation profiles, extracellular exoglycosidase activities, hybridoma growth and mAb production in traditional medium containing 10% (v/v) FBS (fetal bovine serum) [DMEM (Dulbecco's modified Eagle's medium)/FBS] were compared with those obtained in either SFM or CDM (chemically defined medium). SFM and CDM supported higher cell and mAb concentrations than did DMEM/FBS; however, CE (capillary electrophoresis) analyses revealed important changes in mAb glycosylation patterns. Glycosylation patterns showed a broad microheterogeneity in all the media, ranging from complex to high-mannose and paucimannosidic glycans. mAb produced in DMEM/FBS presented 26 glycan structures, whereas a lower glycan microheterogeneity was found for cultures in CDM or SFM, which presented 24 and 22 structures respectively. In DMEM/FBS and CDM, complex glycans without terminal galactose (G0) represented 28 and 32% of the total glycans respectively and 42 and 46% corresponded to galactosylated structures (G1 plus G2) respectively. In contrast, G0 glycans in SFM accounted for 58%, whereas only 28% corresponded to G1 and G2 structures. Extracellular beta-galactosidase activity increased approx. 3-fold in SFM, which can explain the higher G0 content compared with cultures in the other two media. A desirable decrease in sialylated structures, but an undesirable increase in fucosylated forms, was observed in mAb produced in SFM and CDM media. Approxi. 80% of potential mAb glycosylation sites were occupied, regardless of the culture medium used.

  11. ARPE-19 cell growth and cell functions in euglycemic culture media.

    Science.gov (United States)

    Heimsath, Ernest G; Unda, Richard; Vidro, Eileen; Muniz, Albert; Villazana-Espinoza, Elia T; Tsin, Andrew

    2006-12-01

    Human retinal pigmented epithelial cells (ARPE-19) grown in euglycemic media (5.5 mM) had lower cell number, significantly different cell morphology, and lower levels of vascular endothelial growth factor (VEGF) in the culture media than those grown in hyperglycemic media (18 mM) customarily used for culturing ARPE-19 cells. Although it has been shown that within a 24-hour period, all-trans retinoic acid significantly reduces VEGF secretion by retinal pigmented epithelial cells (grown in 18 mM glucose), such an inhibitory effect was not observed in cells grown in 5.5 mM glucose. Our results suggest that ARPE-19 cells grown in euglycemic media exhibit distinctly different cell growth, cell differentiation, and cell functions in comparison to cells grown in hyperglycemic media. Because euglycemic conditions provide a physiological glucose environment, this glucose concentration must be included in all future investigations of the mechanism of diabetic retinopathy when studying VEGF secretion by ARPE-19 cells.

  12. Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea in different culture media

    Directory of Open Access Journals (Sweden)

    Angela de Oliveira

    2010-08-01

    Full Text Available The aim of the present paper was to evaluate cyst formation and growth parameters of Borrelia garinii in a range of media differing in formulation and cost. A qualitative assessment of morphology and motility of B. garinii was conducted. All media were prepared aseptically and used in test tubes or Petri dishes. For each medium, the initial spirochete concentration was standardized to 10³ spirochets/mL. The following culture media were suitable to grow B. garinii: Barbour-Stoenner-Kelly, brain heart infusion and PMR. Growth was minimal at six weeks post-inoculation and maximum spirochete density was observed between 9-12 weeks. Often, the cultures developed cysts of different sizes, isolated or in groups, with a spiraled portion of variable sizes, mainly in unfavorable culture media. Brazilian Lyme disease-like illness, also known as Baggio-Yoshinari syndrome (BYS, is a new and interesting emerging tick-borne disease, caused by Borrelia burgdorferi sensu lato spirochetes, only during its cystic forms. It has been assumed that the peculiar clinical and laboratory features of BYS are consequential to the absence of a human sucker Ixodes ricinus complex tick at risk areas in Brazil, supporting the concept that the borrelia phenotypic expression pattern is modified as it is transmitted through the host.

  13. The Relevance of Cultural and Media Studies to Theatre and Television in Bali

    Directory of Open Access Journals (Sweden)

    Mark Hobart

    2015-10-01

    Full Text Available AbstractA critical approach to Balinese society presents a starkly different picturefrom the representations that Balinese usually tell themselves, whichare largely myths to disguise a painful reality. Bali no longer belongsto Balinese but to international capital, a process of alienation by whichBalinese energetically commoditize their culture while claiming theopposite. Even the frames of reference for discussing what is happeningare inadequate because they predate the rise of contemporary consumercapitalism and the mass media. That is why critical media and culturalstudies, disciplines designed precisely to address such phenomena, arepotentially so relevant for Indonesian intellectuals.

  14. Setting the Agenda: Different strategies of a Mass Media in a model of cultural dissemination

    CERN Document Server

    Pinto, Sebastián; Dorso, Claudio O

    2015-01-01

    Day by day, people exchange opinions about a given new with relatives, friends, and coworkers. In most cases, they get informed about a given issue by reading newspapers, listening to the radio, or watching TV, i.e., through a Mass Media (MM). However, the importance of a given new can be stimulated by the Media by assigning newspaper's pages or time in TV programs. In this sense, we say that the Media has the power to "set the agenda", i.e., it decides which new is important and which is not. On the other hand, the Media can know people's concerns through, for instance, websites or blogs where they express their opinions, and then it can use this information in order to be more appealing to an increasing number of people. In this work, we study different scenarios in an agent-based model of cultural dissemination, in which a given Mass Media has a specific purpose: To set a particular topic of discussion and impose its point of view to as many social agents as it can. We model this by making the Media has a ...

  15. [Phylogenetic diversity of the culturable rare actinomycetes in marine sponge Hymeniacidon perlevis by improved isolation media].

    Science.gov (United States)

    Xin, Yanjuan; Wu, Peichun; Deng, Maicun; Zhang, Wei

    2009-07-01

    Based on the molecular diversity information, seven actinomycete-selective culture media and isolation conditions were modified to isolate and cultivate diverse rare actinomycetes from Hymeniacidon perlevis. Modified, selective cultivation and enrichment media were used, with the addition of an elemental solution of simulating the elemental composition of marine sponge H. perlevis. Restriction Fragment Length Polymorphism (RFLP) analysis of 16S rDNA sequence was used to reveal the diversity of culturable rare actinomycetes. A total of 59 actinomycete strains were isolated from the marine sponge H. perlevis. A total of 27 representative actinomycetes were selected according to their morphological feature, color and pigments. They gave 15 different RFLP patterns after digesting their PCR products of 16s rDNA with Hha I. The results showed that these isolates belonged to 10 genera: Streptomyces, Nocardiopsis, Micromonospora, Cellulosimicrobium, Gordonia, Nocardia, Prauseria, Pseudonocardia , Saccharomonospora and Microbacterium. The modified isolation media and selective cultivation procedures are highly effective in the recovery of culturable actinomycetes from the marine sponge H. perlevis, resulting in the highest diversity of culturable rare actinomycetes from any sponges.

  16. In vitro fertilization (IVF) of mouse ova in HEPES-buffered culture media.

    Science.gov (United States)

    Behr, B R; Stratton, C J; Foote, W D; Knutzen, V; Sher, G

    1990-02-01

    Some major drawbacks of a bicarbonate-buffered culture medium include the requirement of an elaborate incubator system able to maintain a 5% CO2 environment and the inability of the culture medium to maintain a physiological pH range (pH 7.3-7.4) in room air (0.03% CO2). This work resulted in the development of IVF culture media, BB (modified T6) and Hams-HEPES, which use HEPES-buffered systems not requiring the specialized CO2 environment to maintain a physiological pH range in room air. These media generate above-average cleavage rates in in vitro fertilized, superovulated B6CBAF1 mice ova. The effect of heparin and HEPES on cleavage was studied and neither had a significant effect at the concentrations used. Cleavage rates of nonfertilized ova (parthenogenic division) were 9 to 13%. There was no significant difference in parthenogenesis between any of the culture media and it appears to be a function of the strain of mice and the timing between human chorionic gonadotropin (hCG) injection and ovum collection. These results emphasize the need to account for parthenogenesis when determining cleavage rates of in vitro fertilized mouse ova. Also, the results suggest that because of individual mouse differences in cleavage rates, it is important to use an adequate number of mice per group to determine an accurate, average cleavage rate.

  17. The decline of natural sciences in the culture of mass media

    Science.gov (United States)

    Elías, Carlos

    2011-06-01

    This study sets out to determine if the interest in and study of natural sciences is declining in western countries as scientists currently contend. Part one demonstrates how survey results reveal a decline of interest in scientific news in the EU. Part two explores the decline of interest further through examining data such as the number of students interested in scientific subjects and scientific careers. I explore the hypothesis that the lack of interest in scientific subjects is influenced by the culture of the mass media, and the manner in which the media covers scientific items. I examine a range of media outlets, from reality TV shows and TV series, to movies and the press. Many aspects of this paper have been discussed in depth in my book published in 2008: La razón estrangulada (Reason Strangled: the Crisis of Science in Contemporary Society).

  18. Media socialisation and the culturally dominant mode of representation - On the way from the coherent media to semiotic spaces, the example of Popstars

    Directory of Open Access Journals (Sweden)

    Ben Bachmair

    2006-06-01

    Full Text Available The cultural relation between media and its users is undergoing transition. One influence comes from the changing quality of the interrelation of media, commodities and events. This essay is an attempt to reveal the quality of this development by using two strands of argumentation. Firstly the cultural form (Raymond Williams 1975 of the relation between media, commodities, and situation for which Popstars is an example. The second strand of the essay will deal with the socialization process resultant from of this transitional cultural form. Differently expressed with a more actual wording of the available theories, a specific socialization process emerges with the complex of multimedia, intertextual cultural products, landscapes and mediated spaces of childhood.

  19. Differences in gene expression profiles between human preimplantation embryos cultured in two different IVF culture media

    NARCIS (Netherlands)

    Kleijkers, S.H.M.; Eijssen, L.M.T.; Coonen, E.; Derhaag, J.G.; Mantikou, E.; Jonker, M.J.; Mastenbroek, S.; Repping, S.; Evers, J.L.H.; Dumoulin, J.C.M.; van Montfoort, A.P.A.

    2015-01-01

    STUDY QUESTION: Is gene expression in human preimplantation embryos affected by the medium used for embryo culture in vitro during an IVF treatment? SUMMARY ANSWER: Six days of in vitro culture of human preimplantation embryos resulted in medium-dependent differences in expression level of genes inv

  20. The media and its involvement in building the culture of peace or violence. A reflectionfor peace studies.

    Directory of Open Access Journals (Sweden)

    María Gabriela Villar García

    2013-09-01

    Full Text Available The aim of this article is to meditate about the role that the mass media have and have had building the culture of peace and symbolic violence and/or culture from the perspective of peace studies. We highlight the concepts of culture of peace, violence, cultural and symbolic violence from a dialectical perspective. We look for a reflection that make the reader understand about building a culture of peace in a historical context framed by violence worldwide.

  1. Innovative Approaches Using Lichen Enriched Media to Improve Isolation and Culturability of Lichen Associated Bacteria

    Science.gov (United States)

    Biosca, Elena G.; Flores, Raquel; Santander, Ricardo D.; Díez-Gil, José Luis; Barreno, Eva

    2016-01-01

    Lichens, self-supporting mutualistic associations between a fungal partner and one or more photosynthetic partners, also harbor non-photosynthetic bacteria. The diversity and contribution of these bacteria to the functioning of lichen symbiosis have recently begun to be studied, often by culture-independent techniques due to difficulties in their isolation and culture. However, culturing as yet unculturable lichenic bacteria is critical to unravel their potential functional roles in lichen symbiogenesis, to explore and exploit their biotechnological potential and for the description of new taxa. Our objective was to improve the recovery of lichen associated bacteria by developing novel isolation and culture approaches, initially using the lichen Pseudevernia furfuracea. We evaluated the effect of newly developed media enriched with novel lichen extracts, as well as the influence of thalli washing time and different disinfection and processing protocols of thalli. The developed methodology included: i) the use of lichen enriched media to mimic lichen nutrients, supplemented with the fungicide natamycin; ii) an extended washing of thalli to increase the recovery of ectolichenic bacteria, thus allowing the disinfection of thalli to be discarded, hence enhancing endolichenic bacteria recovery; and iii) the use of an antioxidant buffer to prevent or reduce oxidative stress during thalli disruption. The optimized methodology allowed significant increases in the number and diversity of culturable bacteria associated with P. furfuracea, and it was also successfully applied to the lichens Ramalina farinacea and Parmotrema pseudotinctorum. Furthermore, we provide, for the first time, data on the abundance of culturable ecto- and endolichenic bacteria that naturally colonize P. furfuracea, R. farinacea and P. pseudotinctorum, some of which were only able to grow on lichen enriched media. This innovative methodology is also applicable to other microorganisms inhabiting these

  2. Innovative Approaches Using Lichen Enriched Media to Improve Isolation and Culturability of Lichen Associated Bacteria.

    Science.gov (United States)

    Biosca, Elena G; Flores, Raquel; Santander, Ricardo D; Díez-Gil, José Luis; Barreno, Eva

    2016-01-01

    Lichens, self-supporting mutualistic associations between a fungal partner and one or more photosynthetic partners, also harbor non-photosynthetic bacteria. The diversity and contribution of these bacteria to the functioning of lichen symbiosis have recently begun to be studied, often by culture-independent techniques due to difficulties in their isolation and culture. However, culturing as yet unculturable lichenic bacteria is critical to unravel their potential functional roles in lichen symbiogenesis, to explore and exploit their biotechnological potential and for the description of new taxa. Our objective was to improve the recovery of lichen associated bacteria by developing novel isolation and culture approaches, initially using the lichen Pseudevernia furfuracea. We evaluated the effect of newly developed media enriched with novel lichen extracts, as well as the influence of thalli washing time and different disinfection and processing protocols of thalli. The developed methodology included: i) the use of lichen enriched media to mimic lichen nutrients, supplemented with the fungicide natamycin; ii) an extended washing of thalli to increase the recovery of ectolichenic bacteria, thus allowing the disinfection of thalli to be discarded, hence enhancing endolichenic bacteria recovery; and iii) the use of an antioxidant buffer to prevent or reduce oxidative stress during thalli disruption. The optimized methodology allowed significant increases in the number and diversity of culturable bacteria associated with P. furfuracea, and it was also successfully applied to the lichens Ramalina farinacea and Parmotrema pseudotinctorum. Furthermore, we provide, for the first time, data on the abundance of culturable ecto- and endolichenic bacteria that naturally colonize P. furfuracea, R. farinacea and P. pseudotinctorum, some of which were only able to grow on lichen enriched media. This innovative methodology is also applicable to other microorganisms inhabiting these

  3. A model for cross-cultural reciprocal interactions through mass media

    CERN Document Server

    González-Avella, J C; Miguel, M San

    2012-01-01

    We investigate the problem of cross-cultural interactions through mass media in a model where two populations of social agents, each with its own internal dynamics, get information about each other through reciprocal global interactions. As the agent dynamics, we employ Axelrod's model for social influence. The global interaction fields correspond to the statistical mode of the states of the agents and represent mass media messages on the cultural trend originating in each population. Several phases are found in the collective behavior of either population depending on parameter values: two homogeneous phases, one having the state of the global field acting on that population, and the other consisting of a state different from that reached by the applied global field; and a disordered phase. In addition, the system displays nontrivial effects: (i) the emergence of a largest minority group of appreciable size sharing a state different from that of the applied global field; (ii) the appearance of localized orde...

  4. Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture.

    Science.gov (United States)

    Sahu, Neety; Pal, Shilpa; Sapru, Sunaina; Kundu, Joydip; Talukdar, Sarmistha; Singh, N Ibotambi; Yao, Juming; Kundu, Subhas C

    2016-01-01

    Silk protein sericins, in the recent years, find application in cosmetics and pharmaceuticals and as biomaterials. We investigate the potential of sericin, extracted from both mulberry Bombyx mori and different non-mulberry sources, namely, tropical tasar, Antheraea mylitta; muga, Antheraea assama; and eri, Samia ricini, as growth supplement in serum-free culture medium. Sericin supplemented media containing different concentrations of sericins from the different species are examined for attachment, growth, proliferation, and morphology of fibrosarcoma cells. The optimum sericin supplementation seems to vary with the source of sericins. The results indicate that all the sericins promote the growth of L929 cells in serum-free culture media; however, S. ricini sericin seems to promote better growth of cells amongst other non-mulberry sericins.

  5. Transparent polymeric cell culture chip with integrated temperature control and uniform media perfusion

    DEFF Research Database (Denmark)

    Petronis, Sarunas; Stangegaard, Michael; Christensen, C.

    2006-01-01

    Modern microfabrication and microfluidic technologies offer new opportunities in the design and fabrication of miniaturized cell culture systems for online monitoring of living cells. We used laser micromachining and thermal bonding to fabricate an optically transparent, low-cost polymeric chip...... for long-term online cell culture observation under controlled conditions. The chip incorporated a microfluidic flow equalization system, assuring uniform perfusion of the cell culture media throughout the cell culture chamber. The integrated indium-tin-oxide heater and miniature temperature probe linked...... to an electronic feedback system created steady and spatially uniform thermal conditions with minimal interference to the optical transparency of the chip. The fluidic and thermal performance of the chip was verified by finite element modeling and by operation tests under fluctuating ambient temperature conditions...

  6. The mass media destabilizes the cultural homogenous regime in Axelrod's model

    Energy Technology Data Exchange (ETDEWEB)

    Peres, Lucas R; Fontanari, Jose F [Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Caixa Postal 369, 13560-970 Sao Carlos SP (Brazil)

    2010-02-05

    An important feature of Axelrod's model for culture dissemination or social influence is the emergence of many multicultural absorbing states, despite the fact that the local rules that specify the agents interactions are explicitly designed to decrease the cultural differences between agents. Here we re-examine the problem of introducing an external, global interaction-the mass media-in the rules of Axelrod's model: in addition to their nearest neighbors, each agent has a certain probability p to interact with a virtual neighbor whose cultural features are fixed from the outset. Most surprisingly, this apparently homogenizing effect actually increases the cultural diversity of the population. We show that, contrary to previous claims in the literature, even a vanishingly small value of p is sufficient to destabilize the homogeneous regime for very large lattice sizes.

  7. [Use of culture media with sheep serum for maintenance and isolation of Leptospira strains].

    Science.gov (United States)

    Nicolescu, M; Moldoveanu, G

    1976-01-01

    A study was carried out on the effects of a culture medium prepared with sheep serum inactivated at 68 degrees C on Leptospira cultures. Good results were obtained showing that the medium with sheep serum can be used for the cultivation of Leptospira in view of the preparation of antigens for microscopic agglutination and complement fixation. In experiments on the isolation of germs from organocultures and haemocultures the proportion of positive results obtained with sheep serum was smaller than with the media containing rabbit serum.

  8. Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media

    OpenAIRE

    Jozala,Angela Faustino; Pértile, Renata Aparecida Nedel; Santos, Carolina Alves dos; Ebinuma, Valéria de Carvalho Santos; Seckler, Marcelo Martins; Gama, F. M.; Pessoa Júnior, Adalberto

    2015-01-01

    Bacterial cellulose (BC) is used in different fields as a biological material due to its unique properties. Despite there being many BC applications, there still remain many problems associated with bioprocess technology, such as increasing productivity and decreasing production cost. New technologies that use waste from the food industry as raw materials for culture media promote economic advantages because they reduce environmental pollution and stimulate new research for science sustainabi...

  9. Microdrop preparation factors influence culture-media osmolality, which can impair mouse embryo preimplantation development.

    Science.gov (United States)

    Swain, J E; Cabrera, L; Xu, X; Smith, G D

    2012-02-01

    Because media osmolality can impact embryo development, the effect of conditions during microdrop preparation on osmolality was examined. Various sizes of microdrops were prepared under different laboratory conditions. Drops were pipetted directly onto a dish and covered by oil (standard method) or pipetted on the dish, overlaid with oil before removing the underlying media and replaced with fresh media (wash-drop method). Drops were made at 23°C or on a heated stage (37°C) and with or without airflow. Osmolality was assessed at 5 min and 24h. The biological impact of osmolality change was demonstrated by culturing 1-cell mouse embryos in media with varying osmolality. Reduced drop volume, increased temperature and standard method were associated with a significant increase in osmolality at both 5 min and 24h (P-values media with elevated osmolality (>310mOsm/kg; P<0.05). Procedures in the IVF laboratory can alter osmolality and impact embryo development.

  10. Fan Letters to the Cultural Industries: Border Literature about Mass Media

    Directory of Open Access Journals (Sweden)

    Claire Fox

    2001-01-01

    Full Text Available The concentration of the Mexican and U.S. cultural industries in cities outside of the border region and the intermittent outsourcing of Hollywood movies to production facilities in Baja, California, have had a marked impact on the literary practice of "fronterizo" 'border' intellectuals. This essay discusses the theme of the cinema in three narratives by authors from the U.S.-Mexico border region: "Hotel Frontera" ("Border Hotel", by Gabriel Trujillo Muñoz, "Canícula," by Norma Elia Cantú, and "The Magic of Blood," by Dagoberto Gilb. These narratives provide ethnographic information about the reception of nationally distributed mass media in the border region; at the same time they produce a contestatory discourse that challenges the manner in which the border and its populations have been portrayed and employed in the U.S. and Mexican film industries. The study of film culture must take into consideration patterns of consumption as well as production, and literature about mass media is one arena through which it is possible to focus on both of these processes simultaneously. Fronteriza/o writing about cinema reveals a desire to inhabit popular cinematic genres such as film noir and the western while at the same time retaining a critical stance towards them. This ambivalence is understood as a localist response to the marginalization of fronteriza/o cultural production in a bi-national context, rather than as general suspicion toward visual mass media on the part of "traditional" literary intellectuals.

  11. An electrochemical approach to monitor pH change in agar media during plant tissue culture.

    Science.gov (United States)

    Wang, Min; Ha, Yang

    2007-05-15

    In this work, metal oxide microelectrodes were developed to monitor pH change in agar media during plant tissue culture. An antimony wire was produced by a new approach "capillary melt method". The surface of the obtained antimony wire was oxidized in a potassium nitrate melt to fabricate an antimony oxide film for pH sensing. Characterization results show that the oxide layer grown on the wire surface consists of Sb(2)O(3) crystal phase. The sensing response, open-circuit potential, of the electrode has a good linear relationship (R(2)=1.00) with pH value of the test solution. Adding organic compounds into the test media would not affect the linear relationship, although the slope of the lines varied with different ingredients added. The antimony oxide electrodes were employed to continuously monitor pH change of agar culture media during a 2-week plant tissue culture of Dendrobium candidum. The antimony oxide electrode fabricated this way has the advantages of low cost, easy fabrication, fast response, and almost no contamination introduced into the system. It would be suitable for in situ and continuous pH measurement in many bio applications.

  12. Dissolution and aggregation of Cu nanoparticles in culture media: effects of incubation temperature and particles size

    Energy Technology Data Exchange (ETDEWEB)

    Li, Lingxiangyu [Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, State Key Laboratory of Environmental Chemistry and Ecotoxicology (China); Fernández-Cruz, María Luisa; Connolly, Mona [Spanish National Institute for Agricultural and Food Research and Technology - INIA, Department of Environment (Spain); Schuster, Michael [Technische Universität München, Department of Chemistry (Germany); Navas, José María, E-mail: jmnavas@inia.es [Spanish National Institute for Agricultural and Food Research and Technology - INIA, Department of Environment (Spain)

    2015-01-15

    Here, the effects of incubation temperature and particle size on the dissolution and aggregation behavior of copper nanoparticles (CuNPs) in culture media were investigated over 96 h, equivalent to the time period for acute cell toxicity tests. Three CuNPs with the nominal sizes of 25, 50, and 100 nm and one type of micro-sized particles (MPs, ∼500 nm) were examined in culture media used for human and fish hepatoma cell lines acute tests. A large decrease in sizes of CuNPs in the culture media was observed in the first 24 h incubation, and subsequently the sizes of CuNPs changed slightly over the following 72 h. Moreover, the decreasing rate in size was significantly dependent on the incubation temperature; the higher the incubation temperature, the larger the decreasing rate in size. In addition to that, we also found that the release of copper ions depended on the incubation temperature. Moreover, the dissolution rate of Cu particles increased very fast in the first 24 h, with a slight increase over the following 72 h.

  13. Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC.

    Science.gov (United States)

    Adamzyk, Carina; Emonds, Tanja; Falkenstein, Julia; Tolba, René; Jahnen-Dechent, Wilhelm; Lethaus, Bernd; Neuss, Sabine

    2013-01-01

    Orthopedic implants including engineered bone tissue are commonly tested in sheep. To avoid rejection of heterologous or xenogeneic cells, autologous cells are preferably used, that is, ovine mesenchymal stem cells (oMSC). Unlike human MSC, ovine MSC are not well studied regarding isolation, expansion, and characterization. Here we investigated the impact of culture media composition on growth characteristics, differentiation, and surface antigen expression of oMSC. The culture media varied in fetal calf serum (FCS) content and in the addition of supplements and/or additional epidermal growth factor (EGF). We found that FCS strongly influenced oMSC proliferation and that specific combinations of supplemental factors (MCDB-201, ITS-plus, dexamethasone, and L-ascorbic acid) determined the expression of surface epitopes. We compared two published protocols for oMSC differentiation towards the osteogenic, adipogenic, and chondrogenic fate and found (i) considerable donor to donor variations, (ii) protocol-dependent variations, and (iii) variations resulting from the preculture medium composition. Our results indicate that the isolation and culture of oMSC in different growth media are highly variable regarding oMSC phenotype and behaviour. Furthermore, variations from donor to donor critically influence growth rate, surface marker expression, and differentiation.

  14. Development of Colletotrichum gloeosporioides isolated from green pepper in different culture media, temperatures, and light regimes

    Directory of Open Access Journals (Sweden)

    Mello Alexandre Furtado Silveira

    2004-01-01

    Full Text Available Control of anthracnose in green pepper involves the use of resistant varieties and/or fungicides. The selection of varieties and efficient products demands great amounts of conidia as inoculum. It is thus necessary to optimize the production of Colletotrichum gloeosporioides conidia in the laboratory, establishing the best conditions for fungus development. The present study aimed at determining the most favorable culture media, temperature, and light conditions for the production of fungus inoculum. The fungus was isolated from green pepper fruits (Capsicum annuum L. and transferred to four culture media (PDA, oat, filtered pepper extract, and autoclaved pepper extract, under different temperatures (15, 20, 25, 30, and 35ºC and light conditions (24h dark, and 24h light. Colony growth was evaluated after 7 and 12 days of incubation. No differences were found between the culture media. However, the greatest number of conidia was obtained from colonies grown in oat medium at 25ºC. Temperatures of 20 and 25ºC were the most favorable for colony growth and sporulation. Higher sporulation was obtained under incubation in constant light. Cultivation of C. gloeosporioides in oat medium, at 25ºC, and constant light is recommended.

  15. Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC

    Directory of Open Access Journals (Sweden)

    Carina Adamzyk

    2013-01-01

    Full Text Available Orthopedic implants including engineered bone tissue are commonly tested in sheep. To avoid rejection of heterologous or xenogeneic cells, autologous cells are preferably used, that is, ovine mesenchymal stem cells (oMSC. Unlike human MSC, ovine MSC are not well studied regarding isolation, expansion, and characterization. Here we investigated the impact of culture media composition on growth characteristics, differentiation, and surface antigen expression of oMSC. The culture media varied in fetal calf serum (FCS content and in the addition of supplements and/or additional epidermal growth factor (EGF. We found that FCS strongly influenced oMSC proliferation and that specific combinations of supplemental factors (MCDB-201, ITS-plus, dexamethasone, and L-ascorbic acid determined the expression of surface epitopes. We compared two published protocols for oMSC differentiation towards the osteogenic, adipogenic, and chondrogenic fate and found (i considerable donor to donor variations, (ii protocol-dependent variations, and (iii variations resulting from the preculture medium composition. Our results indicate that the isolation and culture of oMSC in different growth media are highly variable regarding oMSC phenotype and behaviour. Furthermore, variations from donor to donor critically influence growth rate, surface marker expression, and differentiation.

  16. Production of red pigments by Monascus ruber in culture media containing corn steep liquor

    Directory of Open Access Journals (Sweden)

    P. S. Hamano

    2006-12-01

    Full Text Available The production of red pigments by Monascus ruber was evaluated utilizing complex culture media composed of glucose or sucrose (10 g/L, corn steep liquor (5 or 10 g/L and monosodium glutamate (0, 5.0, 7.6, 11.4 or 15.2 g/L. Medium containing 10 g/L glucose, 5 g/L corn steep liquor and 7.6 g/L monosodium glutamate resulted the highest values of extracellular red pigment absorbance (20.7 U and productivity (0.35 U/h. This medium also produced better results than using semi-synthetic medium with analytical grade reagents (12.4 U and 0.21 U/h. The cell growth was similar in both media (X @ 6.5 g/L, indicating that the capacity of the cells to produce red pigments was higher in complex culture media. In addition, in the complex culture medium, less of the intracellular red pigments accumulated than in semi-synthetic medium (9.1% and 30%, respectively.

  17. Evaluation of guar gum derivatives as gelling agents for microbial culture media.

    Science.gov (United States)

    Gangotri, Waikhom; Jain-Raina, Ruchi; Babbar, Shashi B

    2012-05-01

    Guar gum, a galactomannan, has been reported to be an inexpensive substitute of agar for microbial culture media. However, its use is restricted probably because of (1) its highly viscous nature even at high temperatures, making dispensing of the media to Petri plates difficult and (2) lesser clarity of the guar gum gelled media than agar media due to impurities present in guar gum. To overcome these problems, three guar gum derivatives, carboxymethyl guar, carboxymethyl hydroxypropyl guar and hydroxypropyl guar, were tested as gelling agents for microbial growth and differentiation. These were also evaluated for their suitability for other routine microbiological methods, such as, enumeration, use of selective and differential media, and antibiotic sensitivity test. For evaluation purpose, growth and differentiation of eight fungi and eight bacteria grown on the media gelled with agar (1.5%), guar gum (4%) or one of the guar gum derivatives (4%), were compared. All fungi and bacteria exhibited normal growth and differentiation on all these media. Generally, growth of most of the fungi was better on guar gum derivatives gelled medium than on agar medium. The enumeration carried out for Serratia sp. and Pseudomonas aeruginosa by serial dilution and pour plate method yielded similar counts in all the treatments. Likewise, the selective succinate medium, specific for P. aeruginosa, did not allow growth of co-inoculated Bacillus sp. even if gelled with guar gum derivatives. The differential medium, Congo red mannitol agar could not differentiate between Agrobacterium tumefaciens and Rhizobium meliloti on color basis, if gelled with guar gum or any of its derivatives However, for antibiotic sensitivity tests for both Gram-positive and -negative bacteria, guar gum and its derivatives were as effective as agar.

  18. Bacterial isolates from the bryozoan Membranipora membranacea: influence of culture media on isolation and antimicrobial activity.

    Science.gov (United States)

    Heindl, Herwig; Thiel, Vera; Wiese, Jutta; Imhoff, Johannes F

    2012-03-01

    From specimens of the bryozoan Membranipora membranacea collected in the Baltic Sea, bacteria were isolated on four different media, which significantly increased the diversity of the isolated groups. All isolates were classified according to 16S rRNA gene sequence analysis and tested for antimicrobial properties using a panel of five indicator strains and six different media. Each medium featured a unique set of isolated phylotypes, and a phylogenetically diverse collection of isolates was obtained. A total of 96 isolates were assigned to 49 phylotypes and 29 genera. Only one-third of the members of these genera had been isolated previously from comparable sources. The isolates were affiliated with Alpha- and Gammaproteobacteria, Bacilli, and Actinobacteria. A comparable large portion of up to 22 isolates, i.e., 15 phylotypes, probably represent new species. Likewise, 47 isolates (approximately 50%) displayed antibiotic activities, mostly against grampositive indicator strains. Of the active strains, 63.8 % had antibiotic traits only on one or two of the growth media, whereas only 12.7 % inhibited growth on five or all six media. The application of six different media for antimicrobial testing resulted in twice the number of positive hits as obtained with only a single medium. The use of different media for the isolation of bacteria as well as the variation of media considered suitable for the production of antibiotic substances significantly enhanced both the number of isolates obtained and the proportion of antibiotic active cultures. Thus the approach described herein offers an improved strategy in the search for new antibiotic compounds.

  19. La convergencia cultural a través de la ecología de medios Understanding Cultural Convergence through Media Ecology

    Directory of Open Access Journals (Sweden)

    Octavio Islas

    2009-10-01

    Full Text Available Antes de Internet cada medio de comunicación tenía funciones y mercados perfectamente definidos. Sin embargo, a consecuencia del formidable desarrollo de Internet y de las comunicaciones digitales, el mismo contenido hoy puede circular a través de distintos medios de comunicación. Esa es la convergencia cultural. El relato transmediático anticipa el advenimiento de nuevos mercados de consumo cultural. Con base en la ecología de medios y particularmente considerando las tesis de Marshall McLuhan, Neil Postman y Henry Jenkins, es analizada la convergencia cultural como complejo ambiente comunicativo. La convergencia cultural modifica los procedimientos de operación de las industrias mediáticas. Los cambios más significativos, sin embargo, se presentan en las comunidades de conocimiento. Before the Internet, the different media had specifically defined functions and markets. However, since the emergence of the Internet and digital communication, the same content can be found right across the media; this is known as cultural convergence. This media crossing anticipates the coming of new markets of cultural consumption. Based on media ecology, with specific reference to the thesis developed by Marshall McLuhan, Neil Postman, and Henry Jenkins, cultural convergence is studied as a complex communication environment. Cultural convergence modifies the operative procedures of media industries. However, the most significant changes can be found within the knowledge communities.

  20. Assessment of developmental potential of caprine cloned embryos with ooplasm replenishment under two culture media.

    Science.gov (United States)

    Gopalakrishna, R; Kumar, Dharmendra; Singh, Ajay Pratap; Pandey, Saurabh Kumar; Ranjan, Rakesh; Sarkhel, B C

    2014-03-12

    The present study was designed to assess the developmental potential of somatic cell nuclear transfer (SCNT) embryos, with and without replenishment of ooplasm into enucleated oocytes, by culturing separately in two culture media. The enucleated oocytes were replenished with exogenous matured ooplasm under replenished nuclear transfer (RNT) method and compared with conventional nuclear transfer (CNT) method without replenishment. The fusion efficiency in RNT group was found to be significantly higher (P < 0.05) than CNT group (59.39 ± 7.36 vs 45.57 ± 3.68%). The completely fused reconstructed oocytes from both groups were cultured separately in research vitro cleave medium (RVCL) and RVCL-Blast medium. The embryonic development of 2 cell, 4 cell, 8-16 cell and 16-32 cell stages in the RNT group was superior to the CNT group regardless of the culture media used (P < 0.05). The embryonic development of the 8-16 cell, 16-32 cell, morula, and blastocyst stages in the RVCL-Blast medium was significantly higher (P < 0.05) than the RVCL alone for both RNT as well as CNT groups. RNT method with RVCL-Blast produced highly significant (P < 0.01) embryonic development for 8-16 cell and 16-32 cell stage when compared to CNT with RVCL. Conclusively, the combination of RNT with RVCL-Blast culture media enabled an overall increase in the embryonic developmental potential.

  1. Evaluation of different cryoprotective agents in maintenance of viability of Helicobacter pylori in stock culture media

    Directory of Open Access Journals (Sweden)

    Daryoush Davoudi Oskouei

    2010-12-01

    Full Text Available Four different cryoprotective supplemented stock media were evaluated for maintaining better survival and recovery of H. pylori type strain NCTC 11637 at two different maintenance temperatures of -20°C and -80°C after one month preservation as frozen stocks. The spread plate colony count method was used to investigate the recovery rate of H. pylori from equally inoculated bacterial suspensions in differently prepared stock cultures. After the preservation of H. pylori for one month in different cryoprotectant-supplemented stock media, the recovery rates for -20°C obtained for stock cultures supplemented with dimethyl sulfoxide (DMSO, polyethylene glycol (PEG, glycerol and glycerol+sucrose, as well as controls with and without human serum alone were 7.13, 6.97, 7.93, 7.99, 6.95 and 0.0 log CFU/ml, respectively. Maintenance of bacteria at -80°C gave statistically higher recovery rates compared to preservation at -20°C with the values of 8.55, 8.24, 8.59, 8.66, 8.01 and 0.0 log CFU/ml for these above mentioned stock cultures. The stock cultures supplemented with glycerol+sucrose and glycerol showed the highest recovery rates, 7.99 and 7.93 for -20°C vs. 8.66 and 8.59 for -80°C respectively, which were statistically different from the others. Our study revealed that H. pylori type strain NCTC 11637 could be better preserved at -80°C than -20°C. The best stock media which supported viability or culturability of bacteria were brain heart infusion broth (BHI+glycerol+human serum and BHI+glycerol+sucrose+human serum, where the latter yielded the higher recovery rate.

  2. Factual accuracy and the cultural context of science in popular media: Perspectives of media makers, middle school students, and university students on an entertainment television program.

    Science.gov (United States)

    Szu, Evan; Osborne, Jonathan; Patterson, Alexis D

    2017-07-01

    Popular media influences ideas about science constructed by the public. To sway media productions, public policy organizations have increasingly promoted use of science consultants. This study contributes to understanding the connection from science consultants to popular media to public outcomes. A science-based television series was examined for intended messages of the creator and consulting scientist, and received messages among middle school and non-science university students. The results suggest the consulting scientist missed an opportunity to influence the portrayal of the cultural contexts of science and that middle school students may be reading these aspects uncritically-a deficiency educators could potentially address. In contrast, all groups discussed the science content and practices of the show, indicating that scientific facts were salient to both media makers and audiences. This suggests popular media may influence the public knowledge of science, supporting concerns of scientists about the accuracy of fictional television and film.

  3. 'Transatlantic Print Culture, 1880-1940: Emerging Media, Emerging Modernisms', edited by Ann Ardis and Patrick Collier

    Directory of Open Access Journals (Sweden)

    Janet Floyd

    2009-11-01

    Full Text Available A review of 'Transatlantic Print Culture, 1880-1940: Emerging Media, Emerging Modernisms', edited by Ann Ardis and Patrick Collier (London: Palgrave Macmillan, 2008. Hardback, 259 pages, £50, ISBN 9780554269.

  4. Ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media.

    Science.gov (United States)

    Gustavsson, J; Ginebra, M P; Engel, E; Planell, J

    2011-12-01

    Solution-mediated surface reactions occur for most calcium phosphate-based biomaterials and may influence cellular response. A reasonable extrapolation of such processes observed in vitro to in vivo performance requires a deep understanding of the underlying mechanisms. We therefore systematically investigated the nature of ion reactivity of calcium-deficient hydroxyapatite (CDHA) by exposing it for different periods of time to standard cell culture media of different chemical composition (DMEM and McCoy medium, with and without osteogenic supplements and serum proteins). Kinetic ion interaction studies of principal extracellular ions revealed non-linear sorption of Ca²⁺ (∼50% sorption) and K⁺ (∼8%) as well as acidification of all media during initial contact with CDHA (48h). Interestingly, inorganic phosphorus (P(i)) was sorbed from McCoy medium (∼50%) or when using osteogenic media containing β-glycerophosphate, but not from DMEM medium. Non-linear sorption data could be perfectly described by pseudo-first-order and pseudo-second-order sorption models. At longer contact time (21 days), and with frequent renewal of culture medium, sorption of Ca²⁺ remained constant throughout the experiment, while sorption of P(i) gradually decreased in McCoy medium. In great contrast, CDHA began to release P(i) slowly with time when using DMEM medium. Infrared spectra showed that CDHA exposed to culture media had a carbonated surface chemistry, suggesting that carbonate plays a key role in the ion reactivity of CDHA. Our data show that different compositions of the aqueous environment may provoke opposite ion reactivity of CDHA, and this must be carefully considered when evaluating the osteoinductive potential of the material.

  5. Children's media culture in the new millennium: mapping the digital landscape.

    Science.gov (United States)

    Montgomery, K C

    2000-01-01

    A new "children's digital media culture" is swiftly moving into place on the Internet. In this article, the author describes the technological, demographic, and market forces shaping this new digital media culture and the rich array of Web sites being created for children and teens. Many nonprofit organizations, museums, educational institutions, and government agencies are playing a significant role in developing online content for children, offering them opportunities to explore the world, form communities with other children, and create their own works of art and literature. For the most part, however, the heavily promoted commercial sites, sponsored mainly by media conglomerates and toy companies, are overshadowing the educational sites. Because of the unique interactive features of the Internet, companies are able to integrate advertising and Web site content to promote "brand awareness" and "brand loyalty" among children, encouraging them to become consumers beginning at a very early age. The possibility that a child's exploration on the Internet might lead to inappropriate content, aggressive advertising, or even dangerous contact with strangers has given rise to a number of efforts to create "safe zones" for children--that is, places in cyberspace where children can be protected from both marketers and predators. Federal legislation now requires parental permission before commercial Web sites can collect personal information from children under age 13. Several companies offer filtering, blocking, and monitoring software to safeguard children from harmful content or predators. Generally lacking in debates concerning children's use of the Internet, however, is a more proactive definition of quality--one that would help ensure the creation and maintenance of Web sites that enhance children's learning and development and not merely keep them from harm. In the concluding section of this article, the author recommends actions to promote development of a quality

  6. Cultural products go online: Comparing the internet and print media on distributions of gender, genre and commercial success

    NARCIS (Netherlands)

    M.N.M. Verboord (Marc)

    2011-01-01

    textabstractThis article examines whether the attention to cultural products on the internet is more democratically structured (in terms of gender and genre distributions) than in traditional print media, and how these types of media attention affect commercial success. For the U.S. fiction book rel

  7. Organizational Information-Seeking in the Digital Era: A Model of New Media Use, Uncertainty Reduction, Identification and Culture

    Science.gov (United States)

    Ju, Ran

    2013-01-01

    This dissertation examines the role of new media in individuals' organizational socialization process across cultures. First, this study has explored individuals' use of new media in their organizational socialization process in two countries, China and the United States, to gain a general understanding of the usage patterns. Second, this study…

  8. Photocatalytic effect of anodic titanium oxide nanotubes on various cell culture media

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Chun-Kang; Hu, Kan-Hung; Wang, Shing-Hoa [National Taiwan Ocean University, Center for Marine Bioenvironment and Biotechnology, Keelung (China); National Taiwan Ocean University, Department of Mechanical and Mechatronic Engineering, Keelung (China); Hsu, Todd [National Taiwan Ocean University, Center for Marine Bioenvironment and Biotechnology, Keelung (China); National Taiwan Ocean University, Institute of Bioscience and Biotechnology, Keelung (China); Tsai, Huei-Ting [National Taiwan Ocean University, Institute of Bioscience and Biotechnology, Keelung (China); Chen, Chien-Chon [National United University, Department of Energy and Resources, Miaoli (China); Liu, Shiu-Mei [National Taiwan Ocean University, Center for Marine Bioenvironment and Biotechnology, Keelung (China); National Taiwan Ocean University, Institute of Marine Biology, Keelung (China); Lin, Tai-Yuan [National Taiwan Ocean University, Institute of Optoelectronic Sciences, Keelung (China); Chen, Chin-Hsing [National Chiao Tong University, Department of Applied Chemistry, Hsinchu (China)

    2011-02-15

    The use of titanium dioxide (TiO{sub 2}) in photodynamic therapy for the treatment of cancer cells has been proposed following studies of cultured cancer cells. In this work, an ordered channel array of anodic titanium oxide (ATO) was fabricated by anodizing titanium foil. The ATO layer of nanotubes with diameters of 100 nm was made in NH{sub 4}F electrolyte by anodization. The photocatalytic effect of ATO was examined on various culture media by ultraviolet A (UV-A) (366 nm) irradiation. After UV-A irradiation of the ATO layer, redox potential of Tris-HCl buffer (pH 7.5) and dilute acrylamide solution increased instantaneously. The redox potential of the serum-containing RPMI1640 medium also increased dramatically, while that of serum-containing MEM and DMEM media increased slightly. The UVA-induced high redox potential was correlated with the greater ability to break down plasmid DNA strands. These phenomena suggest that a culture medium, such as RPMI1640, with a greater ability to produce free radical may be associated with a stronger photocatalytic effect of ATO on cultured cancer cells reported previously. (orig.)

  9. Identifying the essential components of cultural competence in a Chinese nursing context: A qualitative study.

    Science.gov (United States)

    Cai, Duanying; Kunaviktikul, Wipada; Klunklin, Areewan; Sripusanapan, Acharaporn; Avant, Patricia Kay

    2017-06-01

    This qualitative study using semi-structured interviews was conducted to identify the essential components of cultural competence from the perspective of Chinese nurses. A purposive sample of 20 nurse experts, including senior clinical nurses, nurse administrators, and educators in transcultural nursing, was recruited. Using thematic analysis, four themes: awareness, attitudes, knowledge, and skills, with two subthemes for each, were identified. Notably, culture in China was understood in a broad way. The participants' responses focused upon demographic attributes, individuality, and efforts to facilitate quality care rather than on the cultural differences of ethnicity and race and developing the capacity to change discrimination or health disparities. A greater understanding of cultural competence in the Chinese nursing context, in which a dominant cultural group exists, is essential to facilitate the provision of culturally competent care to diverse populations. © 2016 John Wiley & Sons Australia, Ltd.

  10. Parental cultural socialization and educational attainment: Trend effects of traditional cultural capital and media involvement

    NARCIS (Netherlands)

    Kraaykamp, G.L.M.; Notten, N.J.W.R.

    2016-01-01

    This article analyzes long-term developments in parental cultural socialization effects for children's educational attainment. Retrospective information of 3.106 respondents from the Family Survey of the Dutch population are used to address questions on trends in the impact of traditional measures o

  11. Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals.

    Science.gov (United States)

    Chang, Mi-Yoon; Oh, Boram; Rhee, Yong-Hee; Lee, Sang-Hun

    2015-11-01

    Culturing human embryonic stem and induced pluripotent stem cells (hESCs/iPSCs) is one of the most costly and labor-intensive tissue cultures, as media containing expensive factors/cytokines should be changed every day to maintain and propagate undifferentiated hESCs/iPSCs in vitro. We recently reported that doxycycline, an anti-bacterial agent, had dramatic effects on hESC/iPSC survival and promoted self-renewal. In this study, we extended the effects of doxycycline to a more practical issue to save cost and labor in hESC/iPSC cultures. Regardless of cultured cell conditions, hESCs/iPSCs in doxycycline-supplemented media were viable and proliferating for at least 3 days without media change, while none or few viable cells were detected in the absence of doxycycline in the same conditions. Thus, hESCs/iPSCs supplemented with doxycycline can be cultured for a long period of time with media changes at 3-day intervals without altering their self-renewal and pluripotent properties, indicating that doxycycline supplementation can reduce the frequency of media changes and the amount of media required by 1/3. These findings strongly encourage the use of doxycycline to save cost and labor in culturing hESCs/iPSCs.

  12. LIF and TNF alpha concentrations in embryo culture media are predictive for embryo implantation in IVF

    Institute of Scientific and Technical Information of China (English)

    Ursula Zollner; Sonja Bischofs; Irena Lalic; Klaus-Peter Zollner

    2012-01-01

    Objective:There is strong evidence that the cytokines leucemia inhibitory factor (LIF) and tumor necrosis factor (TNF) alpha are related to embryo development and implantation. The aim of this study was to determine the levels of LIF and TNF alpha in embryo culture media and to assess its relationship to the outcome of in-vitro fertilization and embryo transfer. Methods:A total of 99 patients were included in this prospective trial and underwent either IVF or ICSI procedure. A total of 865 oocytes were collected. Embryos were cultured in sequential media until day 5. A standardized morphology evaluation of all embryos, including a detailed pronuclear scoring, was performed daily during this period followed by the replacement of one or two selected embryos. Collected embryo culture fluids of days 3 and 5 were analysed for LIF and TNF alpha on days 3 and 5. Results:Mean TNF alpha concentration in culture media on day 3 was 0.54 and 0.37 pg/mL on day 5 and was significantly lower in women conceiving than in not conceiving (0.43 pg/mL versus 0.59 pg/mL on day 3). Mean LIF concentration on day 3 was 31.5 pg/mL and 35.5 pg/mL on day 5 and was significantly higher in women conceiving (56.2 pg/mL versus 22.2 pg/mL on day 3). Conclusions:The results indicate that LIF could have a function in early embryogenesis and as a factor required for embryo implantation. High TNF alpha concentrations seem to be predictive of implantation failure.

  13. A Sensitive Competitive ELISA for Determination of Biotin in Transformed Yeast Culture Media

    Institute of Scientific and Technical Information of China (English)

    YANGHong

    2003-01-01

    Aim To develop a sensitive competitive ELISA for the determination of biotin in transformed yeast culture media.Methods The ELISA plate was firstly coated with Mycoplasma hyopneumoniae, and then successively incubated with rabbit ami-Mycoplasma hyopneumoniae serum and goat anti-rabbit IgG-biotin to form the solid biotin, which competed with the biotin in the solution (standard or sample) for the limited streptavidin-horse radish peroxidase conjugate. The standard calibration curve for biotin analysis was constructed in the range of 50-2000ng·L-1. Results The detection limit for biotin was found to be 83 ng·L-1 , which waa about 1000 times lower than the lowest determination concenlration in the reported ELISA for biotin analysis. The relative standard deviations for the spiked samples at biotin concerarations of 200 ng·L-1, 500 ng·L-1 , and 1000 ng·L-1 were 24.87%, 6.15%, and 7.86%, respectively, with the average recovery of 101.13%. The wild yeast and its sixty-three transformed yeast culture media were applied to the developed ELISA for the determination of biotin. It was found that the biotin concentrations in more than 85 % of the tested samples were enhanced with different increase factors after transformation. Conclusion Utilization of Mycoplasma hyopnetunoniae as the coating protein improves the precision and accmacy oftbe ELISA assay, which might be used for the biotin assay in other media.

  14. Pollen germination of the walnut cultivar ‛Geisenheim 286’ on different culture media

    Directory of Open Access Journals (Sweden)

    Janković Dragan M.

    2014-01-01

    Full Text Available Pollen of the walnut cultivar ‛Geisenheim 286(27.8% when the germination medium contained 0.8% of agar, 15% of sucrose, 600 ppm of ’ was cultured on germination media containing all possible combinations of sucrose (10, 15 and 20%; agar (0.6 and 0.8%; boric acid (0, 300 and 600 ppm and calcium chloride (0, 50 and 100 ppm. A total of 54 different combinations of germination media were tested in an attempt to establish a suitable culture in vitro pollen germinability The interactions of the concentrations of agar and calcium chloride, boric acid and sucrose, calcium chloride and sucrose, as well as those of boric acid, calcium chloride and sucrose were significant. Pollen germination was maximized media for studying of the walnut. Significant differences in pollen germination were observed in response to changing concentrations of sucrose, boric acid and calcium chloride, but germination was not affected by changes in agar concentration. boric acid and 50 ppm of calcium chloride.

  15. Development of cattleya amethystoglossa x nobilior - orquidaceae in simplified culture media

    Directory of Open Access Journals (Sweden)

    Murilo Goulart Berka

    2014-08-01

    Full Text Available Three simplified, low-cost culture media, prepared with foliar fertilizers, were compared with the commonly used ½ MS-ban (Murashige Skoog medium ½ salt concentration, with banana fruit for the in vitro multiplication of the orchid hybrid Cattleya amethystoglossa x nobilior. The simplified culture media tested were Hy-ban, which is made with 1.33 g L-1 of Hyponex fertilizer (NPK 6.5-6-19; KP-ban, which is made with 0.92 g L-1 of Kristalon™ (NPK 6-12-36 and 0.26 g L-1 of Peters (NPK 30-10-10; and Kcal-ban, which is made with 0.92 g L-1 of Kristalon™ (NPK 6-12-36 and 0.51 g L-1 of Calcinit (NPK 15.5-0-0+Ca 19.0%. Each medium was supplemented with 20 g L-1 of sucrose, 1 g L-1 of activated charcoal, 7 g L-1 of agar-agar and 40 g L-1 of banana fruit, pH 5.6. Plantlets were evaluated after 80 days for the following parameters: number of shoots, shoot length, number of roots, longest root length and fresh weight. While all four media can be used for the studied hybrid, the best medium was Kcal-ban.

  16. Buffalo (Bubalus bubalis in vitro embryo production in two different defined culture media

    Directory of Open Access Journals (Sweden)

    B. Gasparrini

    2011-03-01

    Full Text Available In vitro embryo production (IVEP is largely applied world wide to animal breeding. One of the principal steps of the IVEP is represented by embryo culture (Khurana and Niemann., 2000. In the past, embryos were grown in co-culture systems with other cells such as oviductal epithelial cells, cumulus cells, Buffalo rat liver (BRL and VERO cells (Duszewska et al., 2000. These cells are able to supply the nutrients for embryo development by their replication and metabolism. Nevertheless, the metabolic activity of these cells is also responsible of an early lowering of pH in the culture medium: that needs to be changed every two days. Furthermore, with this culture system it is impossible to standardize all the procedure: in fact the result is dependent from several variables, as the quality of the cells and their concentration in co-culture. The use of defined culture media is necessary to acquire a better comprehension of metabolism and biochemical requirements for IVEP........

  17. Evaluation of economically feasible, natural plant extract-based microbiological media for producing biomass of the dry rot biocontrol strain Pseudomonas fluorescens P22Y05 in liquid culture.

    Science.gov (United States)

    Khalil, Sadia; Ali, Tasneem Adam; Skory, Chris; Slininger, Patricia J; Schisler, David A

    2016-02-01

    The production of microbial biomass in liquid media often represents an indispensable step in the research and development of bacterial and fungal strains. Costs of commercially prepared nutrient media or purified media components, however, can represent a significant hurdle to conducting research in locations where obtaining these products is difficult. A less expensive option for providing components essential to microbial growth in liquid culture is the use of extracts of fresh or dried plant products obtained by using hot water extraction techniques. A total of 13 plant extract-based media were prepared from a variety of plant fruits, pods or seeds of plant species including Allium cepa (red onion bulb), Phaseolus vulgaris (green bean pods), and Lens culinaris (lentil seeds). In shake flask tests, cell production by potato dry rot antagonist Pseudomonas fluorescens P22Y05 in plant extract-based media was generally statistically indistinguishable from that in commercially produced tryptic soy broth and nutrient broth as measured by optical density and colony forming units/ml produced (P ≤ 0.05, Fisher's protected LSD). The efficacy of biomass produced in the best plant extract-based media or commercial media was equivalent in reducing Fusarium dry rot by 50-96% compared to controls. In studies using a high-throughput microbioreactor, logarithmic growth of P22Y05 in plant extract-based media initiated in 3-5 h in most cases but specific growth rate and the time of maximum OD varied as did the maximum pH obtained in media. Nutrient analysis of selected media before and after cell growth indicated that nitrogen in the form of NH4 accumulated in culture supernatants, possibly due to unbalanced growth conditions brought on by a scarcity of simple sugars in the media tested. The potential of plant extract-based media to economically produce biomass of microbes active in reducing plant disease is considerable and deserves further research.

  18. Cultural Self-Awareness as a Crucial Component of Military Cross-Cultural Competence

    Science.gov (United States)

    2013-04-08

    identified personality traits and discussed the supporting evidence as it relates to job performance in multicultural situations. They identified...cultural interaction on a tactical level in the military can be quite different from a business negotiation the business model does provide the most

  19. USDA FSIS and FDA BAM culture methods BBL CHROMagar Salmonella prepared plated and Difco dehydrated culture media.

    Science.gov (United States)

    Ritter, Vicki; Dick, Nancy

    2009-01-01

    BBL and Difco CHROMagar Salmonella (CS) was evaluated internally and externally for the recovery of Salmonella in raw chicken, raw ground beef, raw fish, lettuce, and shell eggs. The raw chicken and ground beef were processed according to the U.S. Department of Agriculture, Food Safety and Inspection Service reference methods. The raw fish, lettuce, and shell eggs were processed according to the U.S. Food and Drug Administration, Bacteriological Analytical Manual procedures. Only raw chicken was found to be naturally contaminated with Salmonella; all other matrixes were seeded with an appropriate dilution of organism to achieve fractionally positive results. Salmonella strains were permitted to equilibrate with the culture-negative matrixes for 48 h at 4 degrees C. Twenty 25 g samples of each food matrix plus 5 uninoculated samples were processed. CS prepared plates (CS PPM) and laboratory prepared plates from dehydrated culture media (CS DCM) were evaluated with the reference method media. A total of 16 positive cultures were obtained from the raw chicken samples, 17 in the raw ground beef, 18 in the raw fish and lettuce, and 11 in the shell eggs. A Chi-square analysis was performed on each of the food matrixes. BBL CS produced comparable results with the reference methods on all matrixes, resulting in a method agreement of 100% based on the Chi-square results. In testing known isolates the sensitivity and specificity was determined to be 94%. Specificity improved to 98% when tetrathionate broth enrichment was used. A negative- and false-positive rate of 6% was found with known isolates. No false negatives were found in testing the food matrixes. The performance of the CS prepared plate and laboratory prepared plate was identical.

  20. 论当代媒介文化的特征%On the Characteristics of Contemporary Media Culture

    Institute of Scientific and Technical Information of China (English)

    刘雄仕

    2012-01-01

    media as the products of culture and the main carrier of culture polymerization, spread and inheritage, it has a profound impact on the cultural development. Media culture is a new cultural form due to the influence of the mass media and the subculture system influenced by the mass media in the overall culture in modem society. Media culture has the huge impact characteristics of mixed complexity, multiple function, and business consumption.%媒介作为文化的生成物,又是文化聚合、播散、传承的主要载体,对文化的发展及走向产生着深刻的影响。媒介文化是因大众媒介的影响而产生的一种新兴的文化形态,是现代社会总体文化中,通过大众传播媒介的影响而建构起的亚文化系统。媒介文化具有混合复杂性、多重功能性、商业消费性、巨大冲击性特征。

  1. Violence in Pop-Culture Media and The Hunger Games as a Prime Artifact

    Directory of Open Access Journals (Sweden)

    Jenna Benson

    2014-10-01

    Full Text Available This paper uses the Critical Discourse Analysis (CDA methodology to analyze the meanings conveyed in relation to violence in Suzanne Collins' popular novel The Hunger Games and its film. As a representational popular­culture artifact marketed to young adults and teens, it is a primary example for the exposure of this age group to the levels of violence regularly displayed in contemporary popular media. This analysis seeks to critique the assertion that the types of violent exposure in the novel and the film are possibly inappropriate for the audience targeted. A new wave of attention and awareness on the part of producers of popular media and people of contemporary society alike is necessary.

  2. Effects of Growth Media on the Diversity of Culturable Fungi from Lichens.

    Science.gov (United States)

    Muggia, Lucia; Kopun, Theodora; Grube, Martin

    2017-05-17

    Microscopic and molecular studies suggest that lichen symbioses contain a plethora of associated fungi. These are potential producers of novel bioactive compounds, but strains isolated on standard media usually represent only a minor subset of these fungi. By using various in vitro growth conditions we are able to modulate and extend the fraction of culturable lichen-associated fungi. We observed that the presence of iron, glucose, magnesium and potassium in growth media is essential for the successful isolation of members from different taxonomic groups. According to sequence data, most isolates besides the lichen mycobionts belong to the classes Dothideomycetes and Eurotiomycetes. With our approach we can further explore the hidden fungal diversity in lichens to assist in the search of novel compounds.

  3. New Additive for Culture Media for Rapid Identification of Aflatoxin-Producing Aspergillus Strains

    Science.gov (United States)

    Fente, C. A.; Ordaz, J. Jaimez; Vázquez, B. I.; Franco, C. M.; Cepeda, A.

    2001-01-01

    A new reliable, fast, and simple method for the detection of aflatoxigenic Aspergillus strains, consisting of the addition of a cyclodextrin (a methylated β-cyclodextrin derivative) to common media used for testing mycotoxin production ability, was developed. We propose the use of this compound as an additive for fungal culture media to enhance the natural fluorescence of aflatoxins. The production of aflatoxins coincided with the presence of a bright blue or blue-green fluorescent area surrounding colonies when observed under long-wavelength (365-nm) UV light after 3 days of incubation at 28°C. The presence of aflatoxins was confirmed by extracting the medium with chloroform and examining the extracts by high-pressure liquid chromatography with fluorescence detection. PMID:11571194

  4. [Evaluation of culture media for detecting the starch hydrolysis reaction in pathovars of Xanthomonas campestris].

    Science.gov (United States)

    Alippi, A M

    1991-01-01

    Sixty strains of different pathovars of Xanthomonas campestris have been tested for the evaluation of various starch agars and compounds of starch degradation on six media: soluble starch, potato insoluble starch, corn insoluble starch, potato amylopectin, corn amylopectin and potato amylose. The purpose of the present investigation was the selection of the most suitable medium for the visualization of the starch hydrolysis test, presenting this reaction as a distinct character between pathovars of the Xanthomonas campestris group. From 60 strains tested, 74% gave positive reactions. Pathovars holcicola, pelargonii, pruni and vitians were negative. Regarding X. campestris pv. vesicatoria cultures, results were variable. Potato and corn insoluble starch agars were the most suitable media for the visualization of the starch hydrolysis reaction and at the same time the most appropriate for direct isolation. Differentiation at species level could be practicable, but within the Xanthomonas campestris group, variation amongst pathovars suggest the unsuitability of the test in spite of the high percentage of positive reactions.

  5. Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes

    Directory of Open Access Journals (Sweden)

    Onofre Sideney Becker

    2001-01-01

    Full Text Available Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA, Czapek-agar (CZP and a complete agar medium (CM under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle were investigated. A completely randomized 3 × 3 (culture media × lighting regime factorial design with four replicates was used. The best mycelial growth and sporulation occurred on the PDA and CM media under continuous illumination (P <= 0,05.

  6. Italianization Accomplished. Forms and Structures of Albanian Television’s Dependency on Italian Media and Culture

    Directory of Open Access Journals (Sweden)

    Paolo Carelli

    2014-06-01

    Full Text Available After the fall of the Berlin Wall and the end of communist regimes in 1989, national media systems of the Eastern European countries belonging to the Soviet bloc began a process of transformation along the way of liberalization and commercialization. In Albania, this process occurred in different phases, but with a common trend, that is the Italian television as a structural, economic and cultural model to inspire. In this article, I try to outline the deep influences and legacies between Italian and Albanian television systems (dating back to the last years of the regime showing how, despite a progressive sliding towards americanization, they remain a typical landmark of Albanian media. From the formats of the first programmes after the regime to the recent appearances of Italian Tv presenters (in particular, from Berlusconi's channels on Albanian private channels, we can observe a subtle but rooted and continuous dependency from the country on the other side of the Adriatic Sea.  

  7. Impact of Dissolved Oxygen during UV-Irradiation on the Chemical Composition and Function of CHO Cell Culture Media.

    Directory of Open Access Journals (Sweden)

    Sarah M Meunier

    Full Text Available Ultraviolet (UV irradiation is advantageous as a sterilization technique in the biopharmaceutical industry since it is capable of targeting non-enveloped viruses that are typically challenging to destroy, as well as smaller viruses that can be difficult to remove via conventional separation techniques. In this work, we investigated the influence of oxygen in the media during UV irradiation and characterized the effect on chemical composition using NMR and LC-MS, as well as the ability of the irradiated media to support cell culture. Chemically defined Chinese hamster ovary cell growth media was irradiated at high fluences in a continuous-flow UV reactor. UV-irradiation caused the depletion of pyridoxamine, pyridoxine, pyruvate, riboflavin, tryptophan, and tyrosine; and accumulation of acetate, formate, kynurenine, lumichrome, and sarcosine. Pyridoxamine was the only compound to undergo complete degradation within the fluences considered; complete depletion of pyridoxamine was observed at 200 mJ/cm2. Although in both oxygen- and nitrogen-saturated media, the cell culture performance was affected at fluences above 200 mJ/cm2, there was less of an impact on cell culture performance in the nitrogen-saturated media. Based on these results, minimization of oxygen in cell culture media prior to UV treatment is recommended to minimize the negative impact on sensitive media.

  8. [Extraction and analysis of chemical components of essential oil in Thymus vulgaris of tissue culture].

    Science.gov (United States)

    Li, Xiao-Dong; Yang, Li; Xu, Shi-Qian; Li, Jian-Guo; Cheng, Zhi-Hui; Dang, Jian-Zhang

    2011-10-01

    To extract the essential oils from the Seedlings, the Aseptic Seedlings and the Tissue Culture Seedlings of Thymus vulgaris and analyze their chemical components and the relative contents. The essential oils were extracted by steam distillation, the chemical components and the relative contents were identified and analyzed by gas chromatography-mass spectrometry (GC/MS) and peak area normalization method. The main chemical components of essential oil in these three samples had no significant difference, they all contained the main components of essential oil in Thymus vulgaris: Thymol, Carvacrol, o-Cymene, gamma-Terpinene, Caryophyllene et al. and only had a slight difference in the relative content. This study provides important theoretical foundation and data reference for further study on production of essential oil in thyme by tissue culture technology.

  9. Components of Executive Control with Advantages for Bilingual Children in Two Cultures

    OpenAIRE

    Bialystok, Ellen; VISWANATHAN, MYTHILI

    2009-01-01

    The present study used a behavioral version of an anti-saccade task, called the ‘faces task’, developed by Bialystok, Craik, & Ryan (2006) to isolate the components of executive functioning responsible for previously reported differences between monolingual and bilingual children and to determine the generality of these differences by comparing bilinguals in two cultures. Three components of executive control were investigated: response suppression, inhibitory control, and cognitive flexibili...

  10. In Vitro Cultivation of 'Unculturable' Oral Bacteria, Facilitated by Community Culture and Media Supplementation with Siderophores.

    Science.gov (United States)

    Vartoukian, Sonia R; Adamowska, Aleksandra; Lawlor, Megan; Moazzez, Rebecca; Dewhirst, Floyd E; Wade, William G

    2016-01-01

    Over a third of oral bacteria are as-yet-uncultivated in-vitro. Siderophores have been previously shown to enable in-vitro growth of previously uncultivated bacteria. The objective of this study was to cultivate novel oral bacteria in siderophore-supplemented culture media. Various compounds with siderophore activity, including pyoverdines-Fe-complex, desferricoprogen and salicylic acid, were found to stimulate the growth of difficult-to-culture strains Prevotella sp. HOT-376 and Fretibacterium fastidiosum. Furthermore, pyrosequencing analysis demonstrated increased proportions of the as-yet-uncultivated phylotypes Dialister sp. HOT-119 and Megasphaera sp. HOT-123 on mixed culture plates supplemented with siderophores. Therefore a culture model was developed, which incorporated 15 μg siderophore (pyoverdines-Fe-complex or desferricoprogen) or 150 μl neat subgingival-plaque suspension into a central well on agar plates that were inoculated with heavily-diluted subgingival-plaque samples from subjects with periodontitis. Colonies showing satellitism were passaged onto fresh plates in co-culture with selected helper strains. Five novel strains, representatives of three previously-uncultivated taxa (Anaerolineae bacterium HOT-439, the first oral taxon from the Chloroflexi phylum to have been cultivated; Bacteroidetes bacterium HOT-365; and Peptostreptococcaceae bacterium HOT-091) were successfully isolated. All novel isolates required helper strains for growth, implying dependence on a biofilm lifestyle. Their characterisation will further our understanding of the human oral microbiome.

  11. El aporte cultural y educativo de la Baja Edad Media The cultural and educational contribution of the Low Middle Ages

    Directory of Open Access Journals (Sweden)

    Clara Tamayo de Serrano

    2007-12-01

    Full Text Available Este trabajo permite conocer por qué la Baja Edad Media fue un periodo de consolidación de varias de las instituciones educativas, de centros de estudio y del surgimiento de diferentes medios para la difusión de la cultura. También se muestra el proceso histórico mediante el cual, a través de los manuscritos, los códices, los volúmenes, los libros, los relieves, las ilustraciones y las bibliotecas, hubo en la Baja Edad Media una luz, que se ha proyectado al Renacimiento, al mundo moderno y al contemporáneo.This paper explains why the Low Middle Ages was a period for the consolidation educational institutions and study centers and for the rise of different means of propagation of culture. It also shows the historical process by which manuscripts, codices, volumes, books, embossments, illustrations and libraries threw a light into the Low Middle Ages and projected it into the Renaissance, the modern world and the contemporary world.

  12. Methodological aspect of research of the process of socialization in media-cultural space of information society

    Directory of Open Access Journals (Sweden)

    N. Y. Hirlina

    2016-03-01

    Full Text Available Integrated within the social and philosophical discourse interdisciplinary methodology, based on the classic philosophical methodology for the analysis of socio-cultural phenomena enables a holistic understanding of the studied phenomenon. From a methodological point of view it is important to determine the social and philosophical understanding of the impact medіa cultural space of personality in conditions of dynamically changing socio-cultural environment. important social and philosophical methodological guideline should be considered on a thesis constant presence in the media culture of human space as being due to the fact that man is a social being, and the information society without media culture as its attribute exists. Philosophical «core» study of the spiritual culture of youth is humanism in its broadest sense, that is, understanding of the studied phenomenon primarily as a multi-dimensional culturing of human values. Submission materialistic determinant factors medіa cultural spiritual space is only possible under the dominance of humanistic values. With all the variety to understanding the spiritual dimension of the relationship of the individual with the socio-cultural environment common dominant philosophical idea of guidelines is the recognition of the spiritual and cultural autonomy rights. Globalization and its associated civilization and processes are seen as foreign in relation to social rights, while the internal spiritual content is cultural processes. Anthropological oriented cultural space of socialization based on interpersonal cultural interaction that produces unique and distinctive personality.

  13. Quantitative isolation of biocontrol agents Trichoderma spp., Gliocladium spp. and actinomycetes from soil with culture media.

    Science.gov (United States)

    Vargas Gil, S; Pastor, S; March, G J

    2009-01-01

    Soil biodiversity plays a key role in the sustainability of agriculture systems and indicates the level of health of soil, especially when considering the richness of microorganisms that are involved in biological control of soilborne diseases. Cultural practices may produce changes in soil microflora, which can be quantified through the isolation of target microorganisms. Rhizosphere soil samples were taken from an assay with different crop rotations and tillage systems, and populations of Trichoderma spp., Gliocladium spp. and actinomycetes were quantified in order to select the general and selective culture media that better reflect the changes of these microbial populations in soil. The most efficient medium for the isolation of Trichoderma spp. and Gliocladium spp. was potato dextrose agar modified by the addition of chloramphenicol, streptomycin and rose bengal, and for actinomycetes was Küster medium, with cycloheximide and sodium propionate.

  14. Dynamics of Coupled Contaminant and Microbial Transport in Heterogeneous Porous Media: Purdue Component

    Energy Technology Data Exchange (ETDEWEB)

    Cushman, J.H.; Madilyn Fletcher

    2000-06-01

    Dynamic microbial attachment/detachment occurs in subsurface systems in response to changing environmental conditions caused by contaminant movement and degradation. Understanding the environmental conditions and mechanisms by which anaerobic bacteria partition between aqueous and solid phases is a critical requirement for designing and evaluating in situ bioremediation efforts. This interdisciplinary research project, of which we report only the Purdue contribution, provides fundamental information on the attachment/detachment dynamics of bacteria in heterogeneous porous media. Fundamental results from the Purdue collaboration are: (a) development of a matched-index method for obtaining 3-D Lagrangian trajectories of microbial sized particles transporting within porous media or microflow cells, (b) application of advanced numerical methods to optimally design a microflow cell for studying anaerobic bacterial attachment/detachment phenomena, (c) development of two types of models for simulating bacterial movement and attachment/detachment in microflow cells and natural porous media, (d) application of stochastic analysis to upscale pore scale microbial attachment/detachment models to natural heterogeneous porous media, and (e) evaluation of the role nonlocality plays in microbial dynamics in heterogeneous porous media

  15. Dynamics of Coupled Contaminant and Microbial Transport in Heterogeneous Porous Media: Purdue Component

    Energy Technology Data Exchange (ETDEWEB)

    Cushman, J.H.

    2000-06-01

    Dynamic microbial attachment/detachment occurs in subsurface systems in response to changing environmental conditions caused by contaminant movement and degradation. Understanding the environmental conditions and mechanisms by which anaerobic bacteria partition between aqueous and solid phases is a critical requirement for designing and evaluating in situ bioremediation efforts. This interdisciplinary research project, of which we report only the Purdue contribution, provides fundamental information on the attachment/detachment dynamics of bacteria in heterogeneous porous media. Fundamental results from the Purdue collaboration are: (a) development of a matched-index method for obtaining 3-D Lagrangian trajectories of microbial sized particles transporting within porous media or microflow cells, (b) application of advanced numerical methods to optimally design a microflow cell for studying anaerobic bacterial attachment/detachment phenomena, (c) development of two types of models for simulating bacterial movement and attachment/detachment in microflow cells and natural porous media, (d) application of stochastic analysis to upscale pore scale microbial attachment/detachment models to natural heterogeneous porous media, and (e) evaluation of the role nonlocality plays in microbial dynamics in heterogeneous porous media.

  16. Parallel experimental design and multivariate analysis provides efficient screening of cell culture media supplements to improve biosimilar product quality.

    Science.gov (United States)

    Brühlmann, David; Sokolov, Michael; Butté, Alessandro; Sauer, Markus; Hemberger, Jürgen; Souquet, Jonathan; Broly, Hervé; Jordan, Martin

    2017-07-01

    Rational and high-throughput optimization of mammalian cell culture media has a great potential to modulate recombinant protein product quality. We present a process design method based on parallel design-of-experiment (DoE) of CHO fed-batch cultures in 96-deepwell plates to modulate monoclonal antibody (mAb) glycosylation using medium supplements. To reduce the risk of losing valuable information in an intricate joint screening, 17 compounds were separated into five different groups, considering their mode of biological action. The concentration ranges of the medium supplements were defined according to information encountered in the literature and in-house experience. The screening experiments produced wide glycosylation pattern ranges. Multivariate analysis including principal component analysis and decision trees was used to select the best performing glycosylation modulators. Subsequent D-optimal quadratic design with four factors (three promising compounds and temperature shift) in shake tubes confirmed the outcome of the selection process and provided a solid basis for sequential process development at a larger scale. The glycosylation profile with respect to the specifications for biosimilarity was greatly improved in shake tube experiments: 75% of the conditions were equally close or closer to the specifications for biosimilarity than the best 25% in 96-deepwell plates. Biotechnol. Bioeng. 2017;114: 1448-1458. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  17. Inhibitory effect of chromogenic culture media on the growth of Rhodotorula: relevance to the diagnosis of Rhodotorula spp. infections.

    Science.gov (United States)

    Bellanger, Anne-Pauline; Grenouillet, Frédéric; François, Nadine; Skana, Florence; Millon, Laurence

    2013-11-01

    With the increasing incidence and diverse etiologies of fungal infections, chromogenic yeast culture media are increasingly used for routine diagnosis. Rhodotorula species, which are characterized by the production of carotenoid pigments, are considered as emerging opportunistic pathogens. We recently diagnosed two fungemia due to Rhodotorula spp. and noticed that in both cases, the yeast failed to grow in subculture on the chromogenic yeast culture medium. This study was thus undertaken to investigate more thoroughly the ability (or inability) of Rhodotorula species to grow on different commercially available chromogenic media for yeast. Eighteen Rhodotorula spp. were checked for their ability to grow on four chromogenic yeast culture media: CHROMagar Candida (BD), Candi 4 Select (Biorad), Brilliance Candida (Oxoid), and Candida ID 2 (BioMerieux). All the Rhodotorula spp. strains grew on Brilliance and Candida ID 2, while only six isolates grew on Candi 4, and seven on CHROMagar. Two chromogenic yeast culture media showed a significant inhibitory effect on the growth of Rhodotorula species. As all Rhodotorula species are resistant to echinocandins and fluconazole, it is essential to isolate and identify these yeast quickly to initiate appropriate amphotericin B antifungal treatment as early as possible. The choice of media for routine use should take into account the ability of different media to allow all emerging fungal pathogens to grow. © 2013 APMIS. Published by John Wiley & Sons Ltd.

  18. Burmese Attitude toward Chinese: Portrayal of the Chinese in Contemporary Cultural and Media Works

    Directory of Open Access Journals (Sweden)

    Min Zin

    2012-01-01

    Full Text Available This paper argues that since at least the mid 1980s, there has been an observable negative attitude among the people of Burma against the Chinese. Such sentiment is not just transient public opinion, but an attitude. The author measures it by studying contemporary cultural and media works as found in legally published expressions, so as to exclude any material rejected by the regime’s censors. The causes of such sentiment are various: massive Chinese migration and purchases of real estate (especially in Upper Burma, Chinese money that is inflating the cost of everything, and cultural “intrusion.” The sentiment extends to the military, as well: the article examines a dozen memoirs of former military generals and finds that Burma’s generals do not trust the Chinese, a legacy of China’s interference in Burma’s civil war until the 1980s. The public outcry over the Myitsone dam issue, however, was the most significant expression of such sentiment since 1969, when anti-Chinese riots broke out in Burma. The relaxation of media restrictions under the new government has allowed this expression to gather steam and spread throughout the country, especially in private weekly journals that are becoming more outspoken and daring in pushing the boundaries of the state’s restrictions.

  19. INTERPASSIVITY AS A SUBJECTIVE EFFECT OF MODERN MEDIA-CULTURE: TO THE STATEMENT OF PROBLEM

    Directory of Open Access Journals (Sweden)

    Olena V. Khodus

    2014-06-01

    Full Text Available The purpose. In this article, research interest is fixed directly on the circumstances theming access personal / private as interpassivity practice of subjective life and creativity, which are mediated by modern media discourse. Methodology. Methodological base of research of contemporary experience of subjectivity is heuristic "ontological turn" in social theory, which allows to consider the subjectivity not as a reality (given, but as a procedural phenomenon, performativity, which exist in conditions of unstable ontologies, world without guarantees, indirect, thus, mass-media representations. Particular emphasis is placed on the deconstruction of traditional oppositions "objective/subjective", "public/private", "active/passive". As a result, a special interest in issues of privacy as "the place" in which the modern subject unstable ontology prefers to realize its self. Scientific novelty. It is proved that in the modern media culture, the subject is not so much a passive spectator, observer, collector information and events to represent relevant interested agents. He also actively reproduces itself in a form of "perceived privacy", which allows special mode of the experiences of private emotions "alone with everybody." Perceived privacy, although the individual reserves the right to individual control over personal space and personal statement (for example, through personal and individual electronic access to digital representations of reality, however, suggests certain techniques of "publication" themselves. It is the question of the interpassivity nature of such practices subjective life and creativity. Conclusions. Proposed research optics suggests that the person remains - the world-making being, who actively constructs their personal reality, however, when this reality is mediated by media representations, life world turns into a ready code, respectively, the active construction becomes superfluous. On the contrary, interpassivity as

  20. “Moros en la costa”: Islam in Spanish visual and media culture

    Directory of Open Access Journals (Sweden)

    Lauren Beck

    2012-10-01

    Full Text Available Los medios de comunicación occidentales caracterizan a los musulmanes como anticuados, fanáticos belicosos que carecen de razón y que exhiben ciertos rasgos físicos. Los polemistas medievales islamificaron a los musulmanes para los lectores occidentales que tenían escaso contacto con el Islam. La islamificación es un discurso que aún domina la representación del Islam, aunque ha evolucionado durante siglos. En este artículo se examina la representación del Islam en los medios de comunicación españoles y de otros países europeos en relación con el orientalismo medieval y moderno, y la aparente fusión de los dos en los medios contemporáneos, y específicamente en los periódicos, la pintura y las viñetas políticas.Palabras clave: medios de comunicación, islam, islamificación_______________________Abstract:Muslims in western media can be portrayed as antiquated, un-modern, bellicose fanatics who lack reason and exhibit certain physical characteristics. Medieval polemics islamified Muslims for westerners who had relatively little contact with Islam. The discourse of islamification is one that still dominates the representation of Islam, although it has evolved over the centuries. This article examines the representation of Islam in Spanish and European media culture in terms of medieval and modern orientalism, and an apparent fusion of these two representational modes in contemporary media representations that include newspapers, painting and political illustration. Keywords: media, islam, islamification

  1. Cost Study of Educational Media Systems and Their Equipment Components. Volume II, Technical Report. Final Report.

    Science.gov (United States)

    General Learning Corp., Washington, DC.

    A common instructional task and a set of educational environments are hypothesized for analysis of media cost data. The analytic structure may be conceptulized as a three-dimensional matrix: the first vector separates costs into production, distribution, and reception; the second vector delineates capital (initial) and operating (annual) costs;…

  2. Spent mushroom substrates as component of growing media for germination and growth of horticultural plants.

    Science.gov (United States)

    Medina, E; Paredes, C; Pérez-Murcia, M D; Bustamante, M A; Moral, R

    2009-09-01

    This research work was conducted in order to investigate the possibility of using spent mushroom substrate (SMS) in the production of horticultural seedlings replacing part of the peat in the growing media. Three vegetable species with different salt sensitivities, the less sensitive being tomato (Lycopersicon esculentum var. Muchamiel), the moderately salt-sensitive being courgette (Cucurbita pepo L. var. Afrodite F1) and the most salt-sensitive being pepper (Capsicum annum L. var. Lamuyo F1) were grown in 12 media containing SMS of two types of mushroom (Agaricus bisporus (SMS-AB) and Pleurotus ostreatus (SMS-PO)) or a mixture of both 50% (v/v) (SMS-50), as well as peat in various ratios. The proportions of each residue in the mixtures elaborated with peat were 25%, 50%, 75% and 100% v/v residue. A substrate of 100% peat was used as control. The experiment was arranged in a completely-randomised design with two replicates per treatment under greenhouse conditions. Prior to sowing, some physical, physico-chemical and chemical properties of the growing media were determined and seed germination and fresh weight of seedling were also measured. In most of the cases, the addition of SMS to the growing media produced an increase in the pH values, salt contents, macro and micronutrient concentrations and a decrease in the water holding capacity contents in comparison to peat, whereas great differences were found in the air capacity values between SMS-based substrates and peat. Up to 75% SMS can be used in mixtures with peat for seed germination of the plant species studied. Regarding the most suitable SMS-based substrates for plant growth, any substrate could be used for tomato seedling production. However, all SMS-AB-based substrates and the media containing low dose of SMS-PO and SMS-50 were adequate for growth of courgette and pepper.

  3. Still and Moving Image Evidences for Mating of Echinococcus granulosus Reared in Culture Media.

    Directory of Open Access Journals (Sweden)

    Tahereh Mohammadzadeh

    2014-03-01

    Full Text Available Echinococcus granulosus cultivation is very important for improvement of different aspect of medical and veterinary researches. Despite many advances in this case, there is a missing link for in vitro life cycle of adult worms and it is fertilization. Regarding the researchers' observations, self-fertilization can be done in worms living in dog intestine, but despite all sorts of experimental techniques, this phenomenon has never been observed in reared worms in culture media. Furthermore, cross fertilization has not been observed in vitro and even in parasites with dog intestinal origin; although it theoretically is possible. During a follow-up of cultivated adult worms, evidences of behaviors similar to self-mating (Type 2 and cross-mating were observed in our lab which will be presented here.Protoscoleces were aseptically removed from sheep hydatid cysts, washed twice with PBS and then cultivated in S.10E.H culture medium. The stages of parasite growth were observed using an inverted microscope for two months and all stages and behaviors were microscopically photographed. Different movies have also been made from these behavioral features.After around 55 days post cultivation, some evidences of behaviors similar to self-mating (Type 2 and cross-mating were observed in some of the mature adult worms. However, fertile eggs in these parasites have never been observed.Regarding the above observations, these parasites show tendency to unsuccessful self-mating/fertilization (type 2 which failure could be due to anatomical position and physiological maturation. Also lack of suitable conditions for self-fertilization causes the worms try to do unsuccessful cross- mating/fertilization in culture media.

  4. A Single Dynamic Metabolic Model Can Describe mAb Producing CHO Cell Batch and Fed-Batch Cultures on Different Culture Media.

    Science.gov (United States)

    Robitaille, Julien; Chen, Jingkui; Jolicoeur, Mario

    2015-01-01

    CHO cell culture high productivity relies on optimized culture medium management under fed-batch or perfused chemostat strategies enabling high cell densities. In this work, a dynamic metabolic model for CHO cells was further developed, calibrated and challenged using datasets obtained under four different culture conditions, including two batch and two fed-batch cultures comparing two different culture media. The recombinant CHO-DXB11 cell line producing the EG2-hFc monoclonal antibody was studied. Quantification of extracellular substrates and metabolites concentration, viable cell density, monoclonal antibody concentration and intracellular concentration of metabolite intermediates of glycolysis, pentose-phosphate and TCA cycle, as well as of energetic nucleotides, were obtained for model calibration. Results suggest that a single model structure with a single set of kinetic parameter values is efficient at simulating viable cell behavior in all cases under study, estimating the time course of measured and non-measured intracellular and extracellular metabolites. Model simulations also allowed performing dynamic metabolic flux analysis, showing that the culture media and the fed-batch strategies tested had little impact on flux distribution. This work thus paves the way to an in silico platform allowing to assess the performance of different culture media and fed-batch strategies.

  5. Ribotyping of strains of Moraxella (Branhamella) catarrhalis cultured from the nasopharynx and middle ear of children with otitis media

    DEFF Research Database (Denmark)

    Brygge, K; Sørensen, C H; Colding, H

    1998-01-01

    . The distribution of these types was found to be almost identical to the distribution among 16 M. catarrhalis strains cultured from middle ear exudates of 16 children with acute otitis media. Ribotype HAPA was found in two-thirds of all the cultures investigated, and 44% of the children harboured more than one......Moraxella (Branhaomella) catarrhalis is frequently present in the nasopharyngeal microflora of small children, especially during episodes of acute otitis media . By means of ribotyping (restriction endonuclease analysis of chromosomal DNA combined with rRNA probing), we studied the genetic...... heterogeneity of 78 cultures of M. catarrhalis obtained from different localities in the nasopharynx of nine young children with secretory otitis media. Using HindIII and PstI as endonucleases, five different ribotypes were recognized, representing at least five different genotypes of M. catarrhalis...

  6. Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii.

    Science.gov (United States)

    Farkas, Joel; Chung, Daehwan; Cha, Minseok; Copeland, Jennifer; Grayeski, Philip; Westpheling, Janet

    2013-01-01

    Methods for efficient growth and manipulation of relatively uncharacterized bacteria facilitate their study and are essential for genetic manipulation. We report new growth media and culture techniques for Caldicellulosiruptor bescii, the most thermophilic cellulolytic bacterium known. A low osmolarity defined growth medium (LOD) was developed that avoids problems associated with precipitates that form in previously reported media allowing the monitoring of culture density by optical density at 680 nm (OD(680)) and more efficient DNA transformation by electroporation. This is a defined minimal medium and does not support growth when a carbon source is omitted, making it suitable for selection of nutritional markers as well as the study of biomass utilization by C. bescii. A low osmolarity complex growth medium (LOC) was developed that dramatically improves growth and culture viability during storage, making it a better medium for routine growth and passaging of C. bescii. Both media contain significantly lower solute concentration than previously published media, allowing for flexibility in developing more specialized media types while avoiding the issues of growth inhibition and cell lysis due to osmotic stress. Plating on LOD medium solidified by agar results in ~1,000-fold greater plating efficiency than previously reported and allows the isolation of discrete colonies. These new media represent a significant advance for both genetic manipulation and the study of biomass utilization in C. bescii, and may be applied broadly across the Caldicellulosiruptor genus.

  7. Evaluation of karst water quality as an early reference of land suitability mapping for vaname shrimp (Litopenaeusvannamei) culture media

    Science.gov (United States)

    Wildan, D. M.; Affandi, R.; Pratiwi, N. T. M.; Krisanti, M.; Ayu, I. P.; Iswantari, A.

    2017-01-01

    Vaname shrimp (Litopenaeusvannamei) is one of the excellent fishery commodities in Indonesia. Vaname shrimp farming can be conducted in low salinity water. Low salinity water sources which could be used as culture media is karst water because it has a high mineral. The research was aimedto evaluate land suitability mapping for pond as the vaname shrimpculture mediaseen from the water quality. Research was conducted in May and August 2016. Water sampling was conducted in several locations; Ancol-Jakarta (seawater), Ciseeng-Bogor (karst water salinity), Ciampea-Bogor (karst freshwater), and Situ Gede Bogor (freshwater). Evaluating the suitability of karst water quality for vaname shrimp culture media, done by the results of karst water quality analysis compared with seawater and SNI 01-7246-2006 on shrimp vaname culture media. The results showed that Karst water of Ciseeng and Ciampea could not directly use as vaname shrimp culture media. It is needed water quality treatment of ozonation and aeration of karst water to improve water quality. Ozonation and aeration treatments were able to improve the quality of karst water up to approach the living quality standard of vaname shrimp media.

  8. mRNA Fragments in In-Vitro Culture Media are Associated with Bovine Preimplantation Embryonic Development

    Directory of Open Access Journals (Sweden)

    Jenna eKropp

    2015-08-01

    Full Text Available In vitro production (IVP systems have been used to bypass problems of fertilization and early embryonic development. However, embryos produced by IVP are commonly selected for implantation based on morphological assessment, which is not a strong indicator of establishment and maintenance of pregnancy. Thus, there is a need to identify additional indicators of embryonic developmental potential. Previous studies have identified microRNA expression in in vitro culture media to be indicative of embryo quality in both bovine and human embryos. Like microRNAs, mRNAs have been shown to be secreted from cells into the extracellular environment, but it is unknown whether or not these RNAs are secreted by embryos. Thus, the objective of the present study was to determine whether mRNAs are secreted into in vitro culture media and if their expression in the media is indicative of embryo quality. In vitro culture medium was generated and collected from both blastocyst and degenerate (those which fail to develop from the morula to blastocyst stage embryos. Small-RNA sequencing revealed that many mRNA fragments were present in the culture media. A total of 17 mRNA fragments were differentially expressed between blastocyst and degenerated conditioned media. Differential expression was confirmed by quantitative real-time PCR for

  9. Optical propagation in linear media atmospheric gases and particles, solid-state components, and water

    CERN Document Server

    Thomas, Michael E

    2006-01-01

    PART I: Background Theory and Measurement. 1. Optical Electromagnetics I. 2. Optical Electromagnetics II. 3. Spectroscopy of Matter. 4. Electrodynamics I: Macroscopic Interaction of Light and Matter. 5. Electrodynamics II: Microscopic Interaction of Light and Matter. 6. Experimental Techniques. PART II: Practical Models for Various Media. 7. Optical Propagation in Gases and the Atmosphere of the Earth. 8. Optical Propagation in Solids. 9. Optical Propagation in Liquids. 10. Particle Absorption and Scatter. 11. Propagation Background and Noise

  10. Water relations in culture media influence maturation of avocado somatic embryos.

    Science.gov (United States)

    Márquez-Martín, Belén; Sesmero, Rafael; Quesada, Miguel A; Pliego-Alfaro, Fernando; Sánchez-Romero, Carolina

    2011-11-15

    Application of transformation and other biotechnological tools in avocado (Persea americana Mill.) is hampered by difficulties in obtaining mature somatic embryos capable of germination at an acceptable rate. In this work, we evaluated the effect of different compounds affecting medium water relations on maturation of avocado somatic embryos. Culture media were characterized with respect to gel strength, water potential and osmotic potential. Improved production of mature somatic embryos was achieved with gelling agent concentrations higher than those considered standard. The osmotic agents such as sorbitol and PEG did not have positive effects on embryo maturation. The number of w-o mature somatic embryos per culture was positively correlated with medium gel strength. Gel strength was significantly affected by gelling agent type as well as by gelling agent and PEG concentration. Medium water potential was influenced by sorbitol concentration; incorporation of PEG to a culture medium did not affect medium water potential. The highest maturation results were achieved on a medium gelled with 10 gl(-1) agar. Moreover, these somatic embryos had improved germination rates. These results corroborate the role of water restriction as a key factor controlling maturation of somatic embryos.

  11. Evaluation and optimization of hepatocyte culture media factors by design of experiments (DoE) methodology.

    Science.gov (United States)

    Dong, Jia; Mandenius, Carl-Fredrik; Lübberstedt, Marc; Urbaniak, Thomas; Nüssler, Andreas K N; Knobeloch, Daniel; Gerlach, Jörg C; Zeilinger, Katrin

    2008-07-01

    Optimization of cell culture media based on statistical experimental design methodology is a widely used approach for improving cultivation conditions. We applied this methodology to refine the composition of an established culture medium for growth of a human hepatoma cell line, C3A. A selection of growth factors and nutrient supplements were systematically screened according to standard design of experiments (DoE) procedures. The results of the screening indicated that the medium additives hepatocyte growth factor, oncostatin M, and fibroblast growth factor 4 significantly influenced the metabolic activities of the C3A cell line. Surface response methodology revealed that the optimum levels for these factors were 30 ng/ml for hepatocyte growth factor and 35 ng/ml for oncostatin M. Additional experiments on primary human hepatocyte cultures showed high variance in metabolic activities between cells from different individuals, making determination of optimal levels of factors more difficult. Still, it was possible to conclude that hepatocyte growth factor, epidermal growth factor, and oncostatin M had decisive effects on the metabolic functions of primary human hepatocytes.

  12. Taking into account socio-cultural component in teaching a foreign (Russian) language

    OpenAIRE

    Kavaliauskas, Mindaugas

    2015-01-01

    The article discusses the problem of implementing socio-cultural component in the process of teaching a foreign (Russian) language. The basic principle of teaching foreign languages focuses on linguistic identity of a student at a concrete study stage. Linguistic identity represents a multi-componential set of language abilities, skills and inclination towards certain language behavior. The principle of focusing teaching on the formation of a learner as a linguistic personality presents a cha...

  13. [Effect of culture media on surface and enzymatic activity in Klebsiella species].

    Science.gov (United States)

    Hostacká, A

    2002-08-01

    The effect of three complex media (KM) [Mueller-Hinton broth (MHB), brain heart infusion (MSI) and proteose peptone (PP)] and one mineral medium (MM) on surface and enzyme activities of five strains Klebsiella species was studied. Klebsiella oxytoca and Klebsiella ornithinolytica had a marked hydrophobic character after growth in MHB, MSI and MM, Klebsiella terrigena only in PP. K. oxytoca and K. ornithinolytica had a higher motility after cultivation in KM compared with MM, the motility of K. terrigena was not affected. The lipolytic activity of all tested strains was highest after growth in MSI and PP. The composition of culture medium affected bacterial parameters tested to a different extent depending on the species.

  14. Chemically differentiating ascorbate-mediated dissolution of quantum dots in cell culture media

    Science.gov (United States)

    Su, Cheng-Kuan; Sun, Yuh-Chang

    2013-02-01

    To investigate the dynamic dissolution of quantum dots (QDs) in cell culture media, in this study we constructed an online automatic analytical system comprising a sequential in-tube solid phase extraction (SPE) device and an inductively coupled plasma (ICP) mass spectrometer. By means of selectively extracting QDs and cadmium ions (Cd2+) onto the interior surface of the polytetrafluoroethylene (PTFE) tube, this novel SPE device could be used to determine the degree of QD dissolution through a simple adjustment of sample acidity. To the best of our knowledge, this study is the first to exploit PTFE tubing as a selective SPE adsorbent for the online chemical differentiation of QDs and Cd2+ ions with the goal of monitoring the phenomenon of QD dissolution in complicated biological matrices. We confirmed the analytical reliability of this system through comparison of the measured Cd-to-QD ratios to the expected values. When analyzing QDs and Cd2+ ions at picomolar levels, a temporal resolution of 8 min was required to load sufficient amounts of the analytes to meet the sensitivity requirements of the ICP mass spectrometer. To demonstrate the practicability of this developed method, we measured the dynamic variations in the Cd-to-QD705 ratio in the presence of ascorbate as a physiological stimulant to generate reactive oxygen species in cell culture media and trigger the dissolution of QDs; our results suggest that the ascorbate-induced QD dissolution was dependent on the time, treatment concentration, and nature of the biomolecule.To investigate the dynamic dissolution of quantum dots (QDs) in cell culture media, in this study we constructed an online automatic analytical system comprising a sequential in-tube solid phase extraction (SPE) device and an inductively coupled plasma (ICP) mass spectrometer. By means of selectively extracting QDs and cadmium ions (Cd2+) onto the interior surface of the polytetrafluoroethylene (PTFE) tube, this novel SPE device could be

  15. Rising bonds: wars, media propaganda, culture of fear and ¨hedocynicism¨

    Directory of Open Access Journals (Sweden)

    Dr. Tasio Camiñas Hernández

    2007-01-01

    Full Text Available This article is a critical reflection based on the study of the events of September 11, 2001 as presented by experts in the Spanish press of reference. This analysis shows evidence of an official propagandistic discourse imposed by the global media companies as well as by the political and economic powers to promote the culture of fear and war within a society deeply rooted in two rising values: cynicism and hedonism (henceforth "hedocynicism". The September 11 events initiated a period of "war on terrorism" characterised by a new conflict between the East and the West, the clash of religionism (radical Christianity versus radical Islamism and a third phase of the globalization, this time based on global armament deterrence.

  16. Bill-Postings as Pervasive Media Culture. Evidence from Edo Central District of Edo State, Nigeria

    Directory of Open Access Journals (Sweden)

    Osakue Stevenson Omoera

    2012-06-01

    Full Text Available This study examines the problem of bill-postings as pervasive media culture, using Edo Central District (ECD of Edo State, Nigeria as a case study. To achieve the set task, it adopts an evaluative methodology. This is complemented by interviews and random photographic snapshots of indiscriminately posted-bills across the district under examination. This paper argues that the indiscriminately posted-bills are eyesores, which apart from not being aesthetically pleasing, exacerbate the environmental management challenge in the ECD. It further contends that the problem is a multilayered one, judging from the different kinds of posters that are commonly posted by diverse groups in society. Consequently, this study asserts that probing the sociological causes and implications of the menace of bill-postings could offer some insights on how to redress the situation. To this end, a number of suggestions are made, with a view to improving the condition of the physical environment in ECD.

  17. DESCRIPTION OF THE CULTURE CHARACTERISTICS OF SOME LIGNICOLOUS BASIDIOMYCETES SPECIES GROWN ON THREE SYNTHETIC MEDIA

    Directory of Open Access Journals (Sweden)

    PETRE Cristiana Virginia

    2013-12-01

    Full Text Available A number of 12 species of lignicolous basidiomycetes were cultivated on potato dextrose agar and malt extract agar, incubated at 25 °C and carefully analyzed for a period of 5 weeks. Lignicolous basidiomycetes are fungi that produce potent enzymes and bioactive secondary metabolites which are successfully used in various industries: bioremediation of polluted environments, biodegradation of toxic substances, pharmacology or agriculture. The objective of this study was the description of the main characteristics of in vitro cultures of some lignicolous basidiomycetes species grown on synthetic media. The main characteristics followed were: the growth rate of the colonies, the general features of the mycelium: shape, color, surface aspect, reverse, the presence of fruiting bodies and exudates and the particular odor.

  18. The Fairy Tale and Its Uses in Contemporary New Media and Popular Culture Introduction

    Directory of Open Access Journals (Sweden)

    Claudia Schwabe

    2016-09-01

    Full Text Available Ever since the beginning of the 21st century, the fairy tale has not only become a staple of the small and silver screen around the globe, it has also migrated into new media, overwhelming audiences with imaginative and spectacular retellings along the way. Indeed, modern fairy-tale adaptations pervading contemporary popular culture drastically subvert, shatter, and alter the public’s understanding of the classic fairy tale. Because of the phenomenally increasing proliferation of fairy-tale transformations in today’s “old” and “new” media, we must reflect upon the significance of the fairy tale for society and its social uses in a nuanced fashion. How, why, and for whom have fairy-tale narratives, characters, and motifs metamorphosed in recent decades? What significant intermedial and intertextual relationships exist nowadays in connection with the fairy tale? This special issue features 11 illuminating articles of 13 scholars in the fields of folklore and fairy-tale studies tackling these and other relevant questions.

  19. USDA FSIS, FDA BAM, AOAC, and ISO culture methods BD BBL CHROMagar Listeria Media.

    Science.gov (United States)

    Ritter, Vicki; Kircher, Susan; Sturm, Krista; Warns, Patty; Dick, Nancy

    2009-01-01

    BBL CHROMagar Listeria Media (CL) was evaluated for detection of Listeria monocytogenes in raw ground beef, smoked salmon, lettuce, and Brie cheese. The recovery of L. monocytogenes on CL was compared to the U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM), U.S. Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS), AOAC, and International Organization for Standardization (ISO) reference-plated media using the recommended pre-enrichments and selective enrichments. Of the 265 food samples tested, 140 were tested using BAM, USDA, or AOAC methods and 125 were tested using ISO methods. CL produced comparable results with the reference methods on all matrixes with a sensitivity of 99.3% and a specificity of 100%. No false negatives were found in testing the food matrixes. There was no statistical difference in recovery based on Chi-square analysis. Known isolates were evaluated, and CL had a sensitivity and specificity of 100%. The results of this study demonstrate that CL is an effective medium for the recovery and detection of L. monocytogenes in raw ground beef, smoked salmon, lettuce, and Brie cheese using FDA BAM, USDA FSIS, AOAC, and ISO culture methods.

  20. Enhanced tetrazolium violet reduction of Salmonella spp. by magnesium addition to the culture media.

    Science.gov (United States)

    Junillon, Thomas; Morand, Lucie; Flandrois, Jean Pierre

    2014-09-01

    Tetrazolium salts (TTZ), such as tetrazolium violet (TV), have been widely used for microbiological studies. The formation of the colored formazan product due to bacterial reduction of the uncolored reagent is extensively exploited to stain cells or colonies in agar or on filters. But an important toxic effect of tetrazolium salts on bacteria exists that limits their use at high concentrations, impairing the efficient staining of the colonies. This is especially the case for Salmonella spp. where we observed, using a classic photometric approach and mathematical modeling of the growth, an important impact of tetrazolium violet on the apparent growth rate below the inhibitory concentration. In this study, we demonstrate that adding magnesium to the medium in the presence of TV leads to a significant increase in the apparent growth rate. Moreover, when higher TV concentrations are used which lead to total inhibition of Salmonella strains, magnesium addition to the culture media allows growth and TV reduction. This effect of magnesium may allow the use of higher TTZ concentrations in liquid growth media and enhance bacteria detection capabilities.

  1. Spreading the Spirit Word: Print Media, Storytelling, and Popular Culture in Nineteenth-Century Spiritualism

    Directory of Open Access Journals (Sweden)

    Simone Natale

    2015-09-01

    Full Text Available Spiritualists in the nineteenth century gave much emphasis to the collection of evidences of scientific meaning. During séances, they used instruments similar to those employed in scientific practice to substantiate their claims. However, these were not the only source of legitimization offered in support of the spiritualist claims. In fact, writers who aimed to provide beliefs in spiritualism with a reliable support relied very often on the testimonies of eyewitness that were reported in a narrative fashion. This article interrogates the role of such anecdotal testimonies in nineteenth-century spiritualism. It argues that they played a twofold role: on one side, they offered a form of evidentiary proof that was complementary to the collection of mechanical-based evidences; on the other side, they circulated in spiritualist publications, creating opportunities to reach a wide public of readers that was made available by the emergence of a mass market for print media. Able to convince, but also to entertain the reader, anecdotal testimonies were perfectly suited for publications in spiritualist books and periodicals. The proliferation of anecdotal testimonies in spiritualist texts, in this regard, hints at the relevance of storytelling in the diffusion of beliefs about religious matters as well as scientific issues within the public sphere. By reporting and disseminating narrative testimonies, print media acted as a channel through which spiritualism’s religious and scientific endeavors entered the field of a burgeoning popular culture.

  2. Simple identification of Trichophyton tonsurans by chlamydospore-like structures produced in culture media.

    Science.gov (United States)

    Mochizuki, Takashi; Anzawa, Kazushi; Sakata, Yuichi; Fujihiro, Machiko

    2013-12-01

    Trichophyton tonsurans is known to be the causative agent of a worldwide epidemic of dermatophytoses among contact sports practitioners, and is spreading among the general population of Japan. Prompt and simple identification of T. tonsurans in diagnostic laboratories is crucial to control infection. The present study evaluated the availability of observation of chlamydospore-like structures grown in culture media as a characteristic for identification of T. tonsurans. Twenty-five strains of T. tonsurans and five strains each of Trichophyton verrucosum, Trichophyton rubrum and Trichophyton mentagrophytes were inoculated on Mycosel agar plates and inoculated Petri dishes were observed by light microscopy from the reverse side. Twenty-three of 25 T. tonsurans strains showed chlamydospore-like structures within 5 days, and all strains at day 8. The numbers of chlamydospore-like structures were very abundant in most strains. The majority of strains of other species showed no chlamydospore-like structures, or very few when present. Positive for chlamydospore-like structures among 15 strains other than T. tonsurans was one strain at day 5 and six strains at day 8. As for the identification of T. tonsurans, presence of chlamydospore-like structures showed 92.0% sensitivity (23/25) and 93.3% specificity (14/15) at day 5, and 100% sensitivity (25/25) and 60.0% specificity (9/15) at day 8. Electron microscopic findings suggest chlamydospore-like structures are not true chlamydospores but are produced by inflation of actively growing hyphae by developing vacuoles in cells. In conclusion, observation of development of chlamydospore-like structures in culture media is the simplest method for identification of T. tonsurans.

  3. High TNT-transforming activity by a mixed culture acclimated and maintained on crude-oil-containing media

    Energy Technology Data Exchange (ETDEWEB)

    Popesku, J. T.; Singh, A.; Ward, O. P. [Waterloo Univ., Dept. of Biology, Waterloo, ON (Canada); Xhao, J-S.; Hawari, J. [National Research Council, Biotechnology Research Inst., Montreal, PQ (Canada)

    2003-05-01

    Results of an investigation of the ability of a culture, which neither originated from a munitions-contaminated site, nor was preselected, nor acclimated to TNT-containing media, to rapidly transform TNT, are presented. Cultivation of a mixed culture in glucose-containing medium for 29 hours resulted in almost complete transformation of 100 ppm TNT, suggesting that some of the enzymes and/or metabolic pathways present in crude-oil-degrading micro-organism have the capacity to transform TNT. Both resting and growing cells were able to transform TNT. When sub-cultured, it was found that TNT was able to support growth of the mixed culture when supplied as sole carbon source, sole nitrogen source, or sole carbon and nitrogen source. It is suggested that high TNT-transforming ability without prior subculture on TNT-containing media may have potential applications in bioremediation of munitions-contaminated soil and wastewater. 20 refs., 3 tabs., 2 figs.

  4. Cultural policy of sed in popularization of progress of USSR in GDR and role of mass media in culture of Eastern German in the seventies

    OpenAIRE

    Nefedov, V.

    2011-01-01

    Nefedov V. V. use materials from Archives foreign policy of the RF and study a question on cultural policy of the SED in the popularization of progress of the USSR in the GDR. Author show role of the mass media in the cultureof the Eastern German in the seventies.Author conclude, in the GDR it was «eastern-germanpatriotism», republic have a advanced culture and «inner emigration». Author as a source of information use materials his conversation with cultural workers.

  5. The neural correlates of persuasion: a common network across cultures and media.

    Science.gov (United States)

    Falk, Emily B; Rameson, Lian; Berkman, Elliot T; Liao, Betty; Kang, Yoona; Inagaki, Tristen K; Lieberman, Matthew D

    2010-11-01

    Persuasion is at the root of countless social exchanges in which one person or group is motivated to have another share its beliefs, desires, or behavioral intentions. Here, we report the first three functional magnetic resonance imaging studies to investigate the neurocognitive networks associated with feeling persuaded by an argument. In the first two studies, American and Korean participants, respectively, were exposed to a number of text-based persuasive messages. In both Study 1 and Study 2, feeling persuaded was associated with increased activity in posterior superior temporal sulcus bilaterally, temporal pole bilaterally, and dorsomedial prefrontal cortex. The findings suggest a discrete set of underlying mechanisms in the moment that the persuasion process occurs, and are strengthened by the fact that the results replicated across two diverse linguistic and cultural groups. Additionally, a third study using region-of-interest analyses demonstrated that neural activity in this network was also associated with persuasion when a sample of American participants viewed video-based messages. In sum, across three studies, including two different cultural groups and two types of media, persuasion was associated with a consistent network of regions in the brain. Activity in this network has been associated with social cognition and mentalizing and is consistent with models of persuasion that emphasize the importance of social cognitive processing in determining the efficacy of persuasive communication.

  6. Superoxol and amylase inhibition tests for distinguishing gonococcal and nongonococcal cultures growing on selective media.

    Science.gov (United States)

    Arko, R J; Odugbemi, T

    1984-01-01

    Two inexpensive screening tests were evaluated singly and in tandem for distinguishing Neisseria gonorrhoeae from other oxidase-positive microorganisms growing on selective gonococcal media. In tests of 728 cultures, including 460 N. gonorrhoeae, 4 Neisseria lactamica, 257 Neisseria meningitidis, and 7 Branhamella catarrhalis, both Superoxol (30% H2O2; J. T. Baker Chemical Co., Phillipsburg, N.J.) and amylase inhibition tests were 100% sensitive (positive) for 20-h cultures of N. gonorrhoeae. Singly, the Superoxol test was 92.7% specific for N. gonorrhoeae, compared with a specificity of 82.3% for the amylase inhibition test. By using tandem screening tests to distinguish gonococci, we achieved an overall specificity of 98.6%. Group A meningococci were the primary source of error in the Superoxol test, with 97% (37 of 38) strains producing gonococcal like reactions for catalase. From 5 to 20% of N. meningitidis serogroups X, Y, Z, and Z' and nontypable strains, as well as about 50% of B. catarrhalis and N. lactamica strains, were also strong catalase producers. Images PMID:6205016

  7. The Neural Correlates of Persuasion: A Common Network across Cultures and Media

    Science.gov (United States)

    Falk, Emily B.; Rameson, Lian; Berkman, Elliot T.; Liao, Betty; Kang, Yoona; Inagaki, Tristen K.; Lieberman, Matthew D.

    2011-01-01

    Persuasion is at the root of countless social exchanges in which one person or group is motivated to have another share its beliefs, desires, or behavioral intentions. Here, we report the first three functional magnetic resonance imaging studies to investigate the neurocognitive networks associated with feeling persuaded by an argument. In the first two studies, American and Korean participants, respectively, were exposed to a number of text-based persuasive messages. In both Study 1 and Study 2, feeling persuaded was associated with increased activity in posterior superior temporal sulcus bilaterally, temporal pole bilaterally, and dorsomedial prefrontal cortex. The findings suggest a discrete set of underlying mechanisms in the moment that the persuasion process occurs, and are strengthened by the fact that the results replicated across two diverse linguistic and cultural groups. Additionally, a third study using region-of-interest analyses demonstrated that neural activity in this network was also associated with persuasion when a sample of American participants viewed video-based messages. In sum, across three studies, including two different cultural groups and two types of media, persuasion was associated with a consistent network of regions in the brain. Activity in this network has been associated with social cognition and mentalizing and is consistent with models of persuasion that emphasize the importance of social cognitive processing in determining the efficacy of persuasive communication. PMID:19925175

  8. Quantitative image analysis as a tool for Yarrowia lipolytica dimorphic growth evaluation in different culture media.

    Science.gov (United States)

    Braga, A; Mesquita, D P; Amaral, A L; Ferreira, E C; Belo, I

    2016-01-10

    Yarrowia lipolytica, a yeast strain with a huge biotechnological potential, capable to produce metabolites such as γ-decalactone, citric acid, intracellular lipids and enzymes, possesses the ability to change its morphology in response to environmental conditions. In the present study, a quantitative image analysis (QIA) procedure was developed for the identification and quantification of Y. lipolytica W29 and MTLY40-2P strains dimorphic growth, cultivated in batch cultures on hydrophilic (glucose and N-acetylglucosamine (GlcNAc) and hydrophobic (olive oil and castor oil) media. The morphological characterization of yeast cells by QIA techniques revealed that hydrophobic carbon sources, namely castor oil, should be preferred for both strains growth in the yeast single cell morphotype. On the other hand, hydrophilic sugars, namely glucose and GlcNAc caused a dimorphic transition growth towards the hyphae morphotype. Experiments for γ-decalactone production with MTLY40-2P strain in two distinct morphotypes (yeast single cells and hyphae cells) were also performed. The obtained results showed the adequacy of the proposed morphology monitoring tool in relation to each morphotype on the aroma production ability. The present work allowed establishing that QIA techniques can be a valuable tool for the identification of the best culture conditions for industrial processes implementation. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media

    Science.gov (United States)

    Kim, Kyukwang; Choi, Duckyu; Lim, Hwijoon; Kim, Hyeongkeun; Jeon, Jessie S.

    2016-01-01

    The detection of bacterial growth in liquid media is an essential process in determining antibiotic susceptibility or the level of bacterial presence for clinical or research purposes. We have developed a system, which enables simplified and automated detection using a camera and a striped pattern marker. The quantification of bacterial growth is possible as the bacterial growth in the culturing vessel blurs the marker image, which is placed on the back of the vessel, and the blurring results in a decrease in the high-frequency spectrum region of the marker image. The experiment results show that the FFT (fast Fourier transform)-based growth detection method is robust to the variations in the type of bacterial carrier and vessels ranging from the culture tubes to the microfluidic devices. Moreover, the automated incubator and image acquisition system are developed to be used as a comprehensive in situ detection system. We expect that this result can be applied in the automation of biological experiments, such as the Antibiotics Susceptibility Test or toxicity measurement. Furthermore, the simple framework of the proposed growth measurement method may be further utilized as an effective and convenient method for building point-of-care devices for developing countries. PMID:27999349

  10. Response of growth, quality parameters and photosynthetic apparatus of endive plant to different culture media

    Directory of Open Access Journals (Sweden)

    Kowalczyk Katarzyna

    2016-06-01

    Full Text Available Endive (Cichorium endivia L. is a leaf vegetable with high vitamin and nutritional values. Therefore, an increase of its cultivation in Poland and other European countries has been observed in recent years. The aim of this research was to study the effect of different growth media on the quality and photosynthetic efficiency of different endive cultivars. The experiment was conducted in controlled greenhouse conditions. Endive was grown in three independent NFT cultivation systems: a standard culture medium (control, A1, a culture medium concentrated three-fold (A2 and a medium concentrated five-fold (A3. Four cultivars were used: ‘Kethel’, ‘Barundi’, ‘Galanti’ and ‘Perceval’. The plants were examined for the number and weight of fully-grown leaves and the weight of roots, dry matter content and chemical quality attributes of endive such as ascorbic acid (AA, total soluble solids (TSS, chlorophyll and proline. In addition, several physiological traits such as modulated chlorophyll a fluorescence were measured.

  11. Evaluation of Culture Media for Isolation of Mycobacterium Species from Human Clinical Specimens

    Science.gov (United States)

    Narang, Rahul; Kandi, Venkataramana

    2016-01-01

    Background: Laboratory diagnosis of tuberculosis has undergone a rapid change during last few years and a number of techniques for culture as well as molecular diagnosis have been used with their respective advantages and disadvantages. Sporadic studies have also reported the isolation of M. tuberculosis on standard blood agar (BA), which at one time was not considered as a suitable medium for mycobacterial culture. The present study was conducted to evaluate the routine use of 5% sheep BA in a mycobacteriology laboratory by comparing isolation rates and time for isolation of mycobacteria from pulmonary and extrapulmonary samples with those on Lowenstein-Jensen (LJ) medium. Material and Methods: BA with antibiotics was prepared and dispensed as slants in McCartney bottles. LJ was prepared and dispensed following the Revised National Tuberculosis Control Programme (RNTCP) guidelines. A total of 500 suspected tuberculosis samples were inoculated on both media in duplicate, incubated at 370C, and observed daily until the appearance of growth. Results: Out of 500 inoculated samples, 99 showed growth on BA and 112 showed growth on LJ medium. Mean growth time on BA was less as compared to LJ medium. The contamination rate was found to be more on BA (7.2%) than on LJ (4.8%). Conclusions:  Mycobacterial growth time was less on BA as compared to LJ.  PMID:27733962

  12. Component

    Directory of Open Access Journals (Sweden)

    Tibor Tot

    2011-01-01

    Full Text Available A unique case of metaplastic breast carcinoma with an epithelial component showing tumoral necrosis and neuroectodermal stromal component is described. The tumor grew rapidly and measured 9 cm at the time of diagnosis. No lymph node metastases were present. The disease progressed rapidly and the patient died two years after the diagnosis from a hemorrhage caused by brain metastases. The morphology and phenotype of the tumor are described in detail and the differential diagnostic options are discussed.

  13. Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods

    DEFF Research Database (Denmark)

    van der Valk, J; Brunner, D; De Smet, K

    2010-01-01

    , reproducible and reduce the use of experimental animals. Good cell culture practice (GCCP) is an attempt to develop a common standard for in vitro methods. The implementation of the use of chemically defined media is part of the GCCP. This will decrease the dependence on animal serum, a supplement......Quality assurance is becoming increasingly important. Good laboratory practice (GLP) and good manufacturing practice (GMP) are now established standards. The biomedical field aims at an increasing reliance on the use of in vitro methods. Cell and tissue culture methods are generally fast, cheap...... with an undefined and variable composition. Defined media supplements are commercially available for some cell types. However, information on the formulation by the companies is often limited and such supplements can therefore not be regarded as completely defined. The development of defined media is difficult...

  14. Visual Communication in Transition: Designing for New Media Literacies and Visual Culture Art Education across Activities and Settings

    Science.gov (United States)

    Zuiker, Steven J.

    2014-01-01

    As an example of design-based research, this case study describes and analyses the enactment of a collaborative drawing and animation studio in a Singapore secondary school art classroom. The design embodies principles of visual culture art education and new media literacies in order to organize transitions in the settings of participation and…

  15. What's Our Position? A Critical Media Literacy Study of Popular Culture Websites with Eighth-Grade Special Education Students

    Science.gov (United States)

    Kesler, Ted; Tinio, Pablo P. L.; Nolan, Brian T.

    2016-01-01

    This article reports on an action research project with 9 eighth-grade special education students in a self-contained classroom in an urban public school. The 1st author, in collaboration with the classroom teacher (3rd author), taught the students a critical media literacy framework to explore popular culture websites. Students learned to analyze…

  16. (Not) Made by the human hand: media consciousness and immediacy in the cultural production of the real

    NARCIS (Netherlands)

    van de Port, M.

    2011-01-01

    Taking its examples from the realm of popular religion and popular culture, this essay shows how sensations of im-mediacy are sought and produced in a great number of fantasy scripts. Some of these scripts seek to undo media-awareness: concealing or denying the involvement of the human hand they pro

  17. Engaging cultural resources to promote mental health in Dutch LSES neighborhoods: study of a community-based participatory media project.

    Science.gov (United States)

    Knibbe, Mare; de Vries, Marten; Horstman, Klasien

    2015-09-28

    Community-based participatory media projects form a promising new strategy for mental health promotion that can help address the mental health-gap identified by the World Health Organization. (2008b) mhGAP, Mental Health Gap Action Programme: Scaling Up Care for Mental, Neurological and Substance Use Disorders. World Health Organization, Geneva. In this article we present an ethnographic study about a participatory media project that was developed to promote mental health in selected Dutch low socio-economic status neighborhoods. Through narrowcastings (group film viewings), participant observation and interviews we mapped the ways in which the media project effected and facilitated the collective sense-making process of the audience with regard to sources of stress impacting mental health and opportunities for action. These determinants of mental health are shaped by cultural dimensions, since the cultural context shapes everyday experiences of stress as well as the resources and skills to manage them. Our analysis shows that the media project engaged cultural resources to challenge stressful social scripts. We conclude that more attention should be paid to cultural narratives in a community to understand how health promotion strategies can support social resilience.

  18. Visual Communication in Transition: Designing for New Media Literacies and Visual Culture Art Education across Activities and Settings

    Science.gov (United States)

    Zuiker, Steven J.

    2014-01-01

    As an example of design-based research, this case study describes and analyses the enactment of a collaborative drawing and animation studio in a Singapore secondary school art classroom. The design embodies principles of visual culture art education and new media literacies in order to organize transitions in the settings of participation and…

  19. What's Our Position? A Critical Media Literacy Study of Popular Culture Websites with Eighth-Grade Special Education Students

    Science.gov (United States)

    Kesler, Ted; Tinio, Pablo P. L.; Nolan, Brian T.

    2016-01-01

    This article reports on an action research project with 9 eighth-grade special education students in a self-contained classroom in an urban public school. The 1st author, in collaboration with the classroom teacher (3rd author), taught the students a critical media literacy framework to explore popular culture websites. Students learned to analyze…

  20. Outgrowth of fibroblast cells from goat skin explants in three different culture media and the establishment of cell lines.

    Science.gov (United States)

    Singh, Mahipal; Sharma, Anil K

    2011-02-01

    Three different commercially available media, known to support human and porcine-specific fibroblast cultures, were tested for their growth potential on goat skin explants. Although outgrowth of fibroblasts was observed in all media tested, irrespective of breed, porcine-specific media exhibited higher rate of growth. Using this media, three fibroblast cell lines (GSF289, GSF737, and GSF2010) from ear skin explants of normal healthy dairy goats of Kiko and Saanen breed were successfully established in culture. Liquid nitrogen stocks of these frozen cells had a viability rate of 96.2% in in vitro cultures. These cells were morphologically indistinguishable from the cell stocks prior to freezing. Analysis of the growth of a fifth passage culture revealed an 'S' shaped growth curve with a population doubling time of 25 h. The cell lines were found negative for microbial, fungal, and mycoplasma contaminations. These goat skin fibroblast lines and the simple method of their isolation and freezing with high rate of viability will provide additional tools to study molecular mechanisms that regulate fibroblast function and for genetic manipulation of small ruminants.

  1. Probing Multi-Component Systems in Porous Media Over a Hierarchy of Length-Scales

    Science.gov (United States)

    Gladden, L. F.; Chandrasekera, T. C.; Fordham, E. J.; Johns, M. L.; Mantle, M. D.; Mitchell, J.; Sankey, M. H.; Sederman, A. J.; Weber, D.

    2008-12-01

    Understanding transport processes in porous media is central to the design and operation of many chemical and physical processes. Applications of two magnetic resonance techniques implemented to study the operation of fixed-bed catalytic reactors are reported here. First, gas and liquid velocity fields within a bead pack is reported, demonstrating that we can now image velocity fields within gas-liquid-solid reactors. In a separate experiment, T1-T2 correlations are shown to gain insight into liquid-surface interactions within catalyst pellets. The two-dimensional relaxation time correlation approach is then extended to the implementation of a chemical-shift (δ)-resolved T2-T1-δ correlation, which has initially been applied to the identification of oil and water fractions in a permeable rock.

  2. Peroxidized mineral oil increases the oxidant status of culture media and inhibits in vitro porcine embryo development.

    Science.gov (United States)

    Martinez, C A; Nohalez, A; Ceron, J J; Rubio, C P; Roca, J; Cuello, C; Rodriguez-Martinez, H; Martinez, E A; Gil, M A

    2017-11-01

    The use of oils with undetected alterations is a long-recognized problem for in vitro embryo production systems. Since peroxides in oils have been associated with reduced embryo production outcomes, our goals were (1) to evaluate the effects of a batch of mineral oil (MO) that was suspected to be altered on the in vitro production of pig embryos and (2) to determine oil peroxide values throughout culture and the transfer of oxidant agents from oil to culture media. Sunflower oil, which has a completely different chemical composition than MO but a higher oxidative status, and unaltered MO were used as controls. Oocyte maturation, fertilization and embryo development were affected differently depending on the oil overlay used. While the suspected MO was not able to sustain in vitro maturation and fertilization, the oocytes incubated in the presence of sunflower oil were matured and fertilized similarly to those of the unaltered MO group. Moreover, the cleavage rate of presumed zygotes cultured under the suspected MO was severely reduced compared with those cultured under the other oils, and none of the cleaved embryos developed to the blastocyst stage. Although the cleavage rates in the sunflower oil and unaltered MO groups were similar, embryos cultured under sunflower oil also failed to develop to the blastocyst stage. Our results revealed that the suspected MO and sunflower oil had similar levels of peroxides and that these levels were much higher than those of the unaltered MO. The total oxidant status was higher in media incubated under peroxidized oils than in fresh media or media incubated without an oil overlay or under unaltered MO, indicating that oxidant agents were transferred to the incubation media. However, unlike the sunflower oil group, the culture media incubated under the suspected MO had high levels of total oxidant status and low levels of hydrogen peroxide and reactive oxygen species, suggesting the presence of other unknown oxidant agents in

  3. Regeneration of viable oil palm plants from protoplasts by optimizing media components, growth regulators and cultivation procedures.

    Science.gov (United States)

    Masani, Mat Yunus Abdul; Noll, Gundula; Parveez, Ghulam Kadir Ahmad; Sambanthamurthi, Ravigadevi; Prüfer, Dirk

    2013-09-01

    Oil palm protoplasts are suitable as a starting material for the production of oil palm plants with new traits using approaches such as somatic hybridization, but attempts to regenerate viable plants from protoplasts have failed thus far. Here we demonstrate, for the first time, the regeneration of viable plants from protoplasts isolated from cell suspension cultures. We achieved a protoplast yield of 1.14×10(6) per gram fresh weight with a viability of 82% by incubating the callus in a digestion solution comprising 2% cellulase, 1% pectinase, 0.5% cellulase onuzuka R10, 0.1% pectolyase Y23, 3% KCl, 0.5% CaCl2 and 3.6% mannitol. The regeneration of protoplasts into viable plants required media optimization, the inclusion of plant growth regulators and the correct culture technique. Microcalli derived from protoplasts were obtained by establishing agarose bead cultures using Y3A medium supplemented with 10μM naphthalene acetic acid, 2μM 2,4-dichlorophenoxyacetic acid, 2μM indole-3-butyric acid, 2μM gibberellic acid and 2μM 2-γ-dimethylallylaminopurine. Small plantlets were regenerated from microcalli by somatic embryogenesis after successive subculturing steps in medium with limiting amounts of growth regulators supplemented with 200mg/l ascorbic acid.

  4. Use of cell culture media for cultivation of the mite pathogenic fungi Neozygites tanajoae and Neozygites floridana.

    Science.gov (United States)

    Delalibera, Italo; Hajek, Ann E; Humber, Richard A

    2003-10-01

    The pathogenic fungus Neozygites tanajoae, one of the most efficient natural enemies of the cassava green mite (CGM) Mononychellus tanajoa in Brazil, was introduced experimentally in Benin in 1998/1999 for the control of CGM. Isolation methods and culture media for in vitro production of N. tanajoae are reported for the first time in this study. Continuous growth of N. tanajoae was achieved using medium NT-1 (IPL-41+5-10% fetal bovine serum+0.3% lactalbumin hydrolysate+0.3% yeastolate). This medium supported production of N. tanajoae up to 1.53 (+ or - 0.08) x 10(7) hyphal bodies/mL after 8 days. The growth of N. tanajoae from Cruz das Almas, Brazil, was compared to the growth of two Neozygites floridana isolates with wider host ranges from North Carolina, US, and Palmira, Colombia, in 11 cell culture media. We demonstrated that differences in nutritional requirements exist between N. tanajoae and the similar species, N. floridana. N. tanajoae is a particularly fastidious species highly specific to CGM and grows well in few media while N. floridana which is less host specific, grows in a broader range of media, including serum free media. N. floridana isolates produced more than 2 x 10(6) hyphal bodies/mL in > or =7 of the 11 media tested. However, the N. tanajoae isolate reached the same final concentration in only 3 media. Cell densities of N. tanajoae also increased slower than in N. floridana isolates in most media. N. tanajoae differed morphologically from the two N. floridana isolates in vitro. Hyphal bodies of eight N. tanajoae isolates are shorter than hyphal bodies of the two N. floridana isolates. The distinction of these two species was initially proposed based on host specificity, genetic and physiological patterns and is supported by the results presented in this study.

  5. Subretinal Pigment Epithelial Deposition of Drusen Components Including Hydroxyapatite in a Primary Cell Culture Model

    Science.gov (United States)

    Pilgrim, Matthew G.; Lengyel, Imre; Lanzirotti, Antonio; Newville, Matt; Fearn, Sarah; Emri, Eszter; Knowles, Jonathan C.; Messinger, Jeffrey D.; Read, Russell W.; Guidry, Clyde; Curcio, Christine A.

    2017-01-01

    Purpose Extracellular deposits containing hydroxyapatite, lipids, proteins, and trace metals that form between the basal lamina of the RPE and the inner collagenous layer of Bruch's membrane are hallmarks of early AMD. We examined whether cultured RPE cells could produce extracellular deposits containing all of these molecular components. Methods Retinal pigment epithelium cells isolated from freshly enucleated porcine eyes were cultured on Transwell membranes for up to 6 months. Deposit composition and structure were characterized using light, fluorescence, and electron microscopy; synchrotron x-ray diffraction and x-ray fluorescence; secondary ion mass spectroscopy; and immunohistochemistry. Results Apparently functional primary RPE cells, when cultured on 10-μm-thick inserts with 0.4-μm-diameter pores, can produce sub-RPE deposits that contain hydroxyapatite, lipids, proteins, and trace elements, without outer segment supplementation, by 12 weeks. Conclusions The data suggest that sub-RPE deposit formation is initiated, and probably regulated, by the RPE, as well as the loss of permeability of the Bruch's membrane and choriocapillaris complex associated with age and early AMD. This cell culture model of early AMD lesions provides a novel system for testing new therapeutic interventions against sub-RPE deposit formation, an event occurring well in advance of the onset of vision loss. PMID:28146236

  6. In vitro culture of Cucumis sativus L. VI. Histological analysis of leaf explants cultured on media with 2, 4-D or 2, 4, 5-T

    OpenAIRE

    Anna Nadolska-Orczyk; Stefan Malepszy

    2014-01-01

    The developmental sequence of callus initiation and somatic embryogenesis in leaf explants of Cucumis sativus cv. Borszczagowski was analysed and compared on media containing two different auxin phenoxy-derivatives (2,4-D and 2,4,5-T) and cytokinin (BAP or 2iP). During the first 20 days of culture on media with 2,4,5-T proliferation of parenchymatic tissue occurred mainly and only small meristematic centers were observed. There was an intensive detachment of parenchymatic cells and dissociati...

  7. Sensitivity and rapidity of blood culture bottles in the detection of cornea organ culture media contamination by bacteria and fungi

    OpenAIRE

    Thuret, G; Carricajo, A.; Chiquet, C.; Vautrin, A C; Celle, N; Boureille, M; Acquart, S; Aubert, G.; Maugery, J; Gain, P.

    2002-01-01

    Aims: To test the bactericidal activity of standard organ culture medium, and to compare the sensitivity and rapidity of blood culture bottles with conventional microbiological methods for detection of bacteria and fungi inoculated in a standard cornea organ culture medium.

  8. Effects of variations in culture media and hormonal treatments upon callus induction potential in endosperm explant of Barringtonia racemosa L.

    Institute of Scientific and Technical Information of China (English)

    Nurul Izzati Osman; Norrizah Jaafar Sidik; Asmah Awal

    2016-01-01

    Objective: To induce callus from the medicinally valuable species, Barringtonia racemosa L.(B. racemosa) whereby the formation of callus is essential for micropropagation studies and in vitro plant secondary metabolites production.Methods: The callus induction potential in B. racemosa was assessed from endosperm explant cultured on different culture media and plant hormonal treatments. Lloyd and Mc Cown’s woody plant medium and Murashige and Skoog’s medium were used in the study as culture media. On the other hand, various concentrations and combinations of2,4-dichlorophenoxyacetic acid(1.0–2.0 mg/L) and kinetin(0.5–2.5 mg/L) had been incorporated in the culture media to exert the effects of auxin and cytokinin on callus induction.Results: From the present study, it was found that the profuse [(1.681 ± 0.770) g fresh weight,(0.239 ± 0.239) g dry weight] and friable callus formation was optimally produced with desirable morphology and considerable percentage of callus induction(56.70%) in endosperm explants cultured on 1.0 mg/L 2,4-dichlorophenoxyacetic acid and 1.5 mg/L kinetin in Murashige and Skoog’s medium.Conclusions: A reliable protocol for inducing callus formation of profuse and friable morphology in endosperm explant of B. racemosa had therefore been successfully established.

  9. Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media

    Directory of Open Access Journals (Sweden)

    Regiane de Fátima Travensolo

    2009-06-01

    Full Text Available DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.DNA Microarray foi desenvolvida para monitorar a expressão de muitos genes de Xylella fastidiosa, permitindo a comparação de duas situações distintas em um único experimento. Os experimentos foram feitos utilizando células de X. fastidiosa cultivada em dois meios de cultura: BCYE e XDM2. Pares de oligonucleotídeos iniciadores foram sintetizados, depositados em lâminas de vidro e o arranjo foi hibridizado contra cDNAs marcados fluorescentemente. Os sinais emitidos foram quantificados, normalizados e os dados foram estatisticamente analisados para verificar os genes diferencialmente expressos. De acordo com nossos dados, 104 genes foram diferencialmente expressos para o meio de cultura XDM2 e 30 genes para o BCYE. No presente estudo, nós demonstramos que a técnica de DNA microarrays eficientemente diferencia genes expressos sob diferentes condições de cultivo.

  10. Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3

    Directory of Open Access Journals (Sweden)

    Kumar Pranaw

    2014-01-01

    Full Text Available Xenorhabdus indica KB-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont Steinernema thermophilum. Since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth were optimized by rotatable central composite design (RCCD using response surface methodology (RSM. The effects of all five chemical components (considered as independent variables, namely tryptone, soya peptone, dextrose, NaCl, and dipotassium phosphate, on protease production (dependent variable were studied, and it was found that tryptone and dextrose had maximum influence on protease production. The protease production was increased significantly by 66.31% under optimal medium conditions (tryptone—5.71, soya peptone—4.9, dextrose—1.45, NaCl—6.08, and dipotassium phosphate—0.47 in g/L. To best of knowledge, there are no reports on optimization of medium component for protease production by X. indica KB-3 using RSM and their application in fibrinolysis. This study will be useful for industrial processes for production of protease enzyme from X. indica KB-3 for its application in the field of agriculture and medicine.

  11. Correlation between plasma component levels of cultured fish and resistance to bacterial infection

    Science.gov (United States)

    Maita, M.; Satoh, K.-I.; Fukuda, Y.; Lee, H.-K.; Winton, J.R.; Okamoto, N.

    1998-01-01

    Mortalities of yellowtail Seriola quinqueradiata artificially infected with Lactococcus garvieae and of rainbow trout Oncorhynchus mykiss artificially infected with Vibrio anguillarum were compared with the levels of plasma components measured prior to challenge. The levels of plasma total cholesterol, free cholesterol and phospholipid of fish surviving infection were significantly higher in both yellowtail and rainbow trout than those of fish which died during the challenge test. Mortality of yellowtail with plasma total cholesterol levels lower than 250 mg/100 ml was significantly higher than that of fish which had cholesterol levels higher than 275 mg/100 ml (p < 0.05). Rainbow trout whose cholesterol was lower than 520 mg/100 ml suffered a significantly higher mortality due to vibriosis than fish having cholesterol levels higher than 560 mg/100 ml (p < 0.005). These results indicate that low levels of plasma lipid components may be an indicator of lowered disease resistance in cultured fish.

  12. Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media

    Directory of Open Access Journals (Sweden)

    Gesiane Ribeiro

    2013-12-01

    Full Text Available The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.

  13. Design of serum-free medium for suspension culture of CHO cells on the basis of general commercial media.

    Science.gov (United States)

    Miki, Hideo; Takagi, Mutsumi

    2015-08-01

    The design of serum-free media for suspension culture of genetically engineered Chinese hamster ovary (CHO) cells using general commercial media as a basis was investigated. Subcultivation using a commercial serum-free medium containing insulin-like growth factor (IGF)-1 with or without FCS necessitated additives other than IGF-1 to compensate for the lack of FCS and improve cell growth. Suspension culture with media containing several combinations of growth factors suggested the effectiveness of addition of both IGF-1 and the lipid signaling molecule lysophosphatidic acid (LPA) for promoting cell growth. Subcultivation of CHO cells in suspension culture using the commercial serum-free medium EX-CELL™302, which contained an IGF-1 analog, supplemented with LPA resulted in gradually increasing specific growth rate comparable to the serum-containing medium and in almost the same high antibody production regardless of the number of generations. The culture with EX-CELL™302 supplemented with LPA in a jar fermentor with pH control at 6.9 showed an apparently higher cell growth rate than the cultures without pH control and with pH control at 6.8. The cell growth in the medium supplemented with aurintricarboxylic acid (ATA), which was much cheaper than IGF-1, in combination with LPA was synergistically promoted similarly to that in the medium supplemented with IGF-1 and LPA. In conclusion, the serum-free medium designed on the basis of general commercial media could support the growth of CHO cells and antibody production comparable to serum-containing medium in suspension culture. Moreover, the possibility of cost reduction by the substitution of IGF-1 with ATA was also shown.

  14. Strategy for selecting disposable bags for cell culture media applications based on a root-cause investigation.

    Science.gov (United States)

    Wood, Joseph; Mahajan, Ekta; Shiratori, Masaru

    2013-01-01

    The use of disposable bags for cell culture media storage has grown significantly in the past decade. Some of the key advantages of using disposable bags relative to non-disposable containers include increased product throughput, decreased cleaning validation costs, reduced risk of cross contamination and lower facility costs. As the scope of use of disposable bags for cell culture applications increases, problematic bags and scenarios should be identified and addressed to continue improving disposables technologies and meet the biotech industry's needs. In this article, we examine a cell culture application wherein media stored in disposable bags is warmed at 37°C before use for cell culture operations. A problematic bag film was identified through a prospective and retrospective cell culture investigation. The investigation provided information on the scope and variation of the issue with respect to different Chinese hamster ovary (CHO) cell lines, cell culture media, and application-specific parameters. It also led to the development of application-specific test methods and enabled a strategy for disposable bag film testing. The strategy was implemented for qualifying an alternative bag film for use in our processes. In this test strategy, multiple lots of 13 bag film types, encompassing eight vendors were evaluated using a three round, cell culture-based test strategy. The test strategy resulted in the determination of four viable bag film options based on the technical data. The results of this evaluation were used to conclude that a volatile or air-quenched compound, likely generated by gamma irradiation of the problematic bag film, negatively impacted cell culture performance.

  15. Comparing Climate Change Coverage in Canadian English and French-Language Print Media: Environmental Values, Media Cultures, and the Narration of Global Warming

    Directory of Open Access Journals (Sweden)

    Eric Dugas

    2012-01-01

    Full Text Available This article compares how climate change is presented in English- andFrench-language print media in Canada. In recent years, climate change has become an increasingly divisive issue, with the media playing a central role in the promotion of competing claims and narratives in the public sphere. Using concepts from environmental sociology and the sociology of journalism, we examine content from six English- and two French-language newspapers from 2007–2008 (N=2,249, and find significant evidence of both convergence and divergence across the language divide. Among the most significant findings are differences in how complexity is handled: English outlets present diverse coveragethat is highly compartmentalized, while the French newspapers present a narrower range of coverage but with thematically richer articles that better link climate change issues to the realms of culture, politics, and economy.

  16. (Dis)connecting Cultures: The Diary of a Short Lived Media Space

    Science.gov (United States)

    Bly, Sara; Harrison, Steve

    Most media space projects were reported as “successes” in that the participants developed or maintained close working relationships. A few reported problematic social results, but were created wholesale with groups with no media space experience. This chapter reports a case where media space users tried and failed to extend the media space to new user communities, building in part on experiences gained in the earlier “successes” and, in part, ignoring the key elements of those successes.

  17. Dispersing multi-component and unstable powders in aqueous media using comb-type anionic polymers

    DEFF Research Database (Denmark)

    Laarz, E.; Kauppi, A.; Andersson, K.M.

    2006-01-01

    of the grafted ethylene oxide side chains showed that the dispersants adsorb onto a MgO surface and infer a repulsion where the range scales with the length of the poly ethylene oxide side chains. The compressibility and the consolidation behavior of MgO particle networks in response to a centrifugal force field...... dispersants also in multi-component powder mixtures with a complex solution and surface chemistry and result in more robust suspensions at significantly higher solids loading compared with e.g., a traditional cationic polyelectrolyte. Direct force measurements on comb-type dispersants with different lengths...

  18. Media Creation and Sharing in Informal, Situated, Authentic Mobile Learning for Local Cultural Diversity Investigation

    Science.gov (United States)

    Walinski, Jacek

    2013-01-01

    New media shape the intensity of intercultural contacts not only through content consumption but also through content creation with modern technologies. Enabling citizens to participate in the content exchange via the Web 2.0 paradigm (audiences as both media consumers and media creators, which is prevalent in modern online services) results in…

  19. Fatal Amusements: Contemplating the Tempest of Contemporary Media and American Culture

    Science.gov (United States)

    Strate, Lance

    2016-01-01

    Our use of the electronic media to conduct serious discourse raises the question of whether "we are amusing ourselves to death," as Neil Postman argued. The approach known as "media ecology," the study of media as environments, which emphasizes the need to understand context and find balance, provides a basis for the analysis…

  20. Numerical analysis of a non equilibrium two-component two-compressible flow in porous media

    KAUST Repository

    Saad, Bilal Mohammed

    2013-09-01

    We propose and analyze a finite volume scheme to simulate a non equilibrium two components (water and hydrogen) two phase flow (liquid and gas) model. In this model, the assumption of local mass non equilibrium is ensured and thus the velocity of the mass exchange between dissolved hydrogen and hydrogen in the gas phase is supposed finite. The proposed finite volume scheme is fully implicit in time together with a phase-by-phase upwind approach in space and it is discretize the equations in their general form with gravity and capillary terms We show that the proposed scheme satisfies the maximum principle for the saturation and the concentration of the dissolved hydrogen. We establish stability results on the velocity of each phase and on the discrete gradient of the concentration. We show the convergence of a subsequence to a weak solution of the continuous equations as the size of the discretization tends to zero. At our knowledge, this is the first convergence result of finite volume scheme in the case of two component two phase compressible flow in several space dimensions.

  1. One pot four component sequential synthesis of hexahydroquinoline derivatives in aqueous media via enaminone intermediates: A green protocol

    Directory of Open Access Journals (Sweden)

    D. Patil

    2017-01-01

    Full Text Available A convenient green chemistry method through one pot four component tandem synthesis of hexahydroquinoline via enaminone intermediate using dimedone, ammonium acetate, aryl aldehydes and malononitrile has been described in aqueous media without the use of any external catalyst. The excess of ammonium acetate used acts as a reagent as well as catalyst. The incorporation of water as solvent along with eradication of external catalyst renders the protocol to comply with the green chemistry aspects. Shorter reaction time, high atom economy, easy work up and purification of products by non-chromatographic method are the crucial features of this methodology. The crystal structure of hexahydroquinoline basically shaped by chromatographic free selective reaction was determined by single crystal X-ray diffraction analysis.

  2. Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media.

    Science.gov (United States)

    Hsu, Hsan-yin; Ohta, Aaron T; Chiou, Pei-Yu; Jamshidi, Arash; Neale, Steven L; Wu, Ming C

    2010-01-21

    Optoelectronic tweezers (OET), based on light-induced dielectrophoresis, has been shown as a versatile tool for parallel manipulation of micro-particles and cells (P. Y. Chiou, A. T. Ohta and M. C. Wu, Nature, 2005, 436, 370-372). However, the conventional OET device cannot operate in cell culture media or other high-conductivity physiological buffers due to the limited photoconductivity of amorphous silicon. In this paper, we report a new phototransistor-based OET (Ph-OET). Consisting of single-crystalline bipolar junction transistors, the Ph-OET has more than 500x higher photoconductivity than amorphous silicon. Efficient cell trapping of live HeLa and Jurkat cells in Phosphate Buffered Saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) has been demonstrated using a digital light projector, with a cell transport speed of 33 microm/sec, indicating a force of 14.5 pN. Optical concentration of cells and real-time control of individually addressable cell arrays have also been realized. Precise control of separation between two cells has also been demonstrated. We envision a new platform for single cell studies using Ph-OET.

  3. Comparative Study on Antistaphylococcal Activity of Lipopeptides in Various Culture Media

    Directory of Open Access Journals (Sweden)

    Maciej Jaśkiewicz

    2017-08-01

    Full Text Available Staphylococcus aureus bacteria are one of the leading microorganisms responsible for nosocomial infections as well as being the primary causative pathogen of skin and wound infections. Currently, the therapy of staphylococcal diseases faces many difficulties, due to a variety of mechanisms of resistance and virulence factors. Moreover, a number of infections caused by S. aureus are connected with biofilm formation that impairs effectiveness of the therapy. Short cationic lipopeptides that are designed on the basis of the structure of antimicrobial peptides are likely to provide a promising alternative to conventional antibiotics. Many research groups have proved a high antistaphylococcal potential of lipopeptides, however, the use of different protocols for determination of antimicrobial activity may be the reason for inconsistency of the results. The aim of this study was to learn how the use of various bacteriological media as well as solvents may affect activity of lipopeptides and their cyclic analogs. Obtained results showed a great impact of these variables. For example, cyclic analogs were more effective when dissolved in an aqueous solution of acetic acid and bovine serum albumin (BSA. The greater activity against planktonic cultures was found in brain-heart infusion broth (BHI and tryptic-soy broth (TSB, while the antibiofilm activity was higher in the Mueller-Hinton medium.

  4. Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media

    Directory of Open Access Journals (Sweden)

    Ana Karla de Souza Abud

    2017-05-01

    Full Text Available Biomass from lignocellulosic material constitutes a promising energy alternative and without competing with food production. However, pretreatments are required for conversion into sugars which release hexoses, pentoses and other sugars, coupled to inhibitors. Current analysis focuses on ethanol production with the three major inhibitors of lignocellulosic biomass pretreatment, namely, acetic acid, furfural and 5-hydroxymetilfurfural (HMF, and investigates the influence of a mixture of these inhibitors on fermentation by Pichia stipits, using commercial xylose as the only carbon source, through a full factorial 23 + 3 design of experiments (DOE. Fermentations were conducted in a laboratory scale, at 150 rpm and 72h, in a complex culture media with xylose and different inhibitor concentrations, based on the experimental analysis of sugarcane bagasse and 2.107 cell mL-1 of initial concentration of the microorganism. Experimental results showed a significant influence of acetic acid concentration, which must be at the lowest possible level, with no influence of furfural and hydroxymethyl furfural respectively up to concentrations 2.25 and 0.75 g L-1.

  5. Optimization of culture media for enhancing gamma-linolenic acid production by Mucor hiemalis

    Directory of Open Access Journals (Sweden)

    Mina Mohammadi Nasr

    2016-03-01

    Full Text Available Introduction: g-linolenic acid is an essential fatty acid in human nutrition. In the present study, production of g-linolenic acid by Mucor hiemalis PTCC 5292 was evaluated in submerged fermentation. Materials and methods: The fermentation variables were chosen according to the fractional factorial design and further optimized via full factorial method. Four significant variables, glucose, peptone, ammonium nitrate and pH were selected for the optimization studies. The design consisted of total 16 runs consisting of runs at two levels for each factor with three replications of the center points. Results: The analysis of variance and three-dimensional response surface plot of effects indicated that variables were regarded to be significant for production of g-linolenic acid by Mucor hiemalis. Results indicated that fermentation at the optimum conditions (100 g/l glucose concentration; 1 g/l peptone; 1 g/l ammonium nitrate, and pH of 4.5 enhanced the g-linolenic acid production up to 709 mg/l. Discussion and conclusion: The results of this study indicated that higher g-linolenic acid yield can be achieved in a simple medium at high glucose and ammonium nitrate, low peptone concentrations and acidic pH by Mucor hiemalis PTCC 5292. This simple and low cost optimization condition of culture media can be applied for g-linolenic acid production at higher scale for pharmaceutical and nutritional industries. 

  6. Alcohol Control in Cuba: Preventing Countervailing Cultural and Mass Media Influences.

    Science.gov (United States)

    González-Menéndez, Ricardo Á

    2016-07-01

    Harmful use of alcohol-the prime gateway drug to other addictions-is also a problem in Cuba, even though the National Program for Prevention of Harmful Use of Alcohol includes the most effective measures used in analogous programs around the world. As a participant in the program's committee and empirical observer of its accomplishments and unaccomplished goals, I draw attention to the community's attitude of tolerance toward intoxication manifested by the lack of proportional consequences, and I insist on the need to broaden the community's understanding of the risks of non-social drinking, which in Latin America is practically limited to alcoholism and its complications. This undervalues the damage wreaked by unpredictable and dangerous behavior under the influence, as well as the suffering of codependents and other "passive drinkers," and the adverse effects of even social drinking. KEYWORDS Alcohol abuse/prevention and control, alcohol consumption, alcohol drinking/culture, alcoholism, drinking behavior, behavior and behavior mechanisms, social determinants of health, social reinforcement, mass media, communication, Cuba.

  7. Transport of Organic Oil Components from Immobile and Bypassed Oil in Heterogeneous Porous Media

    Energy Technology Data Exchange (ETDEWEB)

    Huseby, O.; Haugan, A.; Sagen, J.; Muller, J.; Bennett, B.; Larter, S.; Kikkinides, E.S.; Stubos, A.K.; Yousefian, F.; Thovert, J.-F.; Adler, P.M.

    2001-12-01

    An experimental study, as well as theoretical and numerical models, are used to validate a methodology to exploit conventional geochemical data with regard to the concentration profiles of organic components occurring naturally in hydrocarbon reservoir oils. The experiment was designed to study transport of organic compounds from immobile oil during water injection. A homogeneous oil-filled core sample was made heterogeneous by drilling a hole through its central axis and filling it with a highly permeable material. Under the present conditions, diffusion coefficients are the most important parameters controlling the transport, and the effect of partitioning could be accounted for by a simple normalization. The experimental results are well described by a simple 2-D analytical model which assumes instantaneous removal of solutes from the oil-water interface. The experimental results are also well described by two numerical models, of which one is a full featured reservoir scale model, suitable for applications of the methodology to reservoir scale cases. (author)

  8. Transport of Organic Oil Components from Immobile and Bypassed Oil in Heterogeneous Porous Media

    Energy Technology Data Exchange (ETDEWEB)

    Huseby, O.; Haugan, A.; Sagen, J.; Muller, J.; Bennett, B.; Larter, S.; Kikkinides, E.S.; Stubos, A.K.; Yousefian, F.; Thovert, J.-F.; Adler, P.M.

    2001-12-01

    An experimental study, as well as theoretical and numerical models, are used to validate a methodology to exploit conventional geochemical data with regard to the concentration profiles of organic components occurring naturally in hydrocarbon reservoir oils. The experiment was designed to study transport of organic compounds from immobile oil during water injection. A homogeneous oil-filled core sample was made heterogeneous by drilling a hole through its central axis and filling it with a highly permeable material. Under the present conditions, diffusion coefficients are the most important parameters controlling the transport, and the effect of partitioning could be accounted for by a simple normalization. The experimental results are well described by a simple 2-D analytical model which assumes instantaneous removal of solutes from the oil-water interface. The experimental results are also well described by two numerical models, of which one is a full featured reservoir scale model, suitable for applications of the methodology to reservoir scale cases. (author)

  9. Ideological and Temporal Components of Network Polarization in Online Political Participatory Media

    CERN Document Server

    Garcia, David; Schweighofer, Simon; Serdült, Uwe; Schweitzer, Frank

    2015-01-01

    Political polarization is traditionally analyzed through the ideological stances of groups and parties, but it also has a behavioral component that manifests in the interactions between individuals. We present an empirical analysis of the digital traces of politicians in politnetz.ch, a Swiss online platform focused on political activity, in which politicians interact by creating support links, comments, and likes. We analyze network polarization as the level of intra- party cohesion with respect to inter-party connectivity, finding that supports show a very strongly polarized structure with respect to party alignment. The analysis of this multiplex network shows that each layer of interaction contains relevant information, where comment groups follow topics related to Swiss politics. Our analysis reveals that polarization in the layer of likes evolves in time, increasing close to the federal elections of 2011. Furthermore, we analyze the internal social network of each party through metrics related to hierar...

  10. The Effect of Growth Media on Cucumber Yield and its Uptake of Some Nutrient Elements in Soilless Culture

    Directory of Open Access Journals (Sweden)

    N. Alifar

    2010-06-01

    Full Text Available Hydroponic growth systems are expanding due to their advantages such as maximum yield, healthy crop production and reduced labour requirement. Vegetable in each stage of the plant growth needs adequate nutrients in different growing media. The objective of this study was to examine the effect of some growth media on N, K and Mg uptake by greenhouse cucumber. This study was carried out in the research greenhouse of Islamic Azad University-Khorasgan branch, using a completely randomized design with 5 treatments, 4 replications and 10 plants in each replication. The Five different growth media consited of pure cocopeat, perlite-cocopeat (50-50 v/v, perlite-cocopeat-peatmoss (50-20-30 v/v and 50-30-20 and perlite-peatmoss. Physical and chemical properties and some nutrients concentrations such as N, K and Mg as well as fruit yield were examined. Although no significant difference was found among the studied growth media in the fruit yield of cucumber, the highest yield was obtained for cocopeat. Significant difference (P < 0.05 was observed in the plant biomass among the growth media. The highest biomass was obtained for the cocopeat and perlite-cocopeat media. No significant difference was found among the growth media in concentrations of N, K and Mg although the highest concentrations of N and K in the second and third stages of sampling were obtained in cocopeat. Shoot concentration of Mg at the first and second stages of sampling was more than the critical deficiency level. The best concentration of Mg was obtained in cocopeat. In general, the best nutrient concentration and yield were found in cocopeat substrate which can be used as an appropriate growing cucumber media for in soilless culture.

  11. Development of an animal-component free medium for vero cells culture.

    Science.gov (United States)

    Rourou, Samia; van der Ark, Arno; van der Velden, Tiny; Kallel, Héla

    2009-01-01

    This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. Statistical experimental design was applied to identify crucial nutrients that affect cell growth. Using Medium 199 or MEM as a basal medium, a serum-free medium (SFM) referred as IPT-SFM that only enclosed transferrin as a component of animal origin was developed at first. Then, the composition of IPT-SFM was further improved to obtain an animal-component free medium named IPT-AFM. IPT-AFM contains M199 as a basal medium, plant hydrolysates, epidermal growth factor, ethanolamine, ferric citrate, and vitamin C. Among various plant hydrolysates, specific combinations of soy (Hypep 1510) and wheat gluten (Hypeps 4601 and 4605) hydrolysates, were identified to promote cell growth; whereas individual Hypeps had a minor positive effect on cell growth. Nevertheless, the removal of serum did influence cell attachment. Coating tissue-culture flasks with teleostean, a product extracted from cold water fish skin, had not only enhanced cell attachment but also improved cell growth performance in static cultures. Different non-animal proteases were also assessed as an alternative to trypsin. TrypLE Select, a recombinant trypsin, gave the best cell growth performances. Kinetics of cell growth in IPT-AFM were investigated in T-flasks, cell growth was comparable with that obtained in MEM+10% fetal calf serum (FCS). A mean cell division number equal to 2.26 +/- 0.18 and a specific growth rate micro 0.019 +/- 0.003 h(-1) were achieved in IPT-AFM. (c) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009.

  12. Geographic differences in time to culture conversion in liquid media: Tuberculosis Trials Consortium study 28. Culture conversion is delayed in Africa.

    Directory of Open Access Journals (Sweden)

    William R Mac Kenzie

    Full Text Available BACKGROUND: Tuberculosis Trials Consortium Study 28, was a double blind, randomized, placebo-controlled, phase 2 clinical trial examining smear positive pulmonary Mycobacterium tuberculosis. Over the course of intensive phase therapy, patients from African sites had substantially delayed and lower rates of culture conversion to negative in liquid media compared to non-African patients. We explored potential explanations of this finding. METHODS: In TBTC Study 28, protocol-correct patients (n = 328 provided spot sputum specimens for M. tuberculosis culture in liquid media, at baseline and weeks 2, 4, 6 and 8 of study therapy. We compared sputum culture conversion for African and non-African patients stratified by four baseline measures of disease severity: AFB smear quantification, extent of disease on chest radiograph, cavity size and the number of days to detection of M. tuberculosis in liquid media using the Kaplan-Meier product-limit method. We evaluated specimen processing and culture procedures used at 29 study laboratories serving 27 sites. RESULTS: African TB patients had more extensive disease at enrollment than non-African patients. However, African patients with the least disease by the 4 measures of disease severity had conversion rates on liquid media that were substantially lower than conversion rates in non-African patients with the greatest extent of disease. HIV infection, smoking and diabetes did not explain delayed conversion in Africa. Some inter-site variation in laboratory processing and culture procedures within accepted practice for clinical diagnostic laboratories was found. CONCLUSIONS: Compared with patients from non-African sites, African patients being treated for TB had delayed sputum culture conversion and lower sputum conversion rates in liquid media that were not explained by baseline severity of disease, HIV status, age, smoking, diabetes or race. Further investigation is warranted into whether modest

  13. Particulate metal bioaccessibility in physiological fluids and cell culture media: Toxicological perspectives.

    Science.gov (United States)

    Leclercq, Bérénice; Alleman, Laurent Yves; Perdrix, Esperanza; Riffault, Véronique; Happillon, Mélanie; Strecker, Alain; Lo-Guidice, Jean-Marc; Garçon, Guillaume; Coddeville, Patrice

    2017-07-01

    According to the literature, tiny amounts of transition metals in airborne fine particles (PM2.5) may induce proinflammatory cell response through reactive oxygen species production. The solubility of particle-bound metals in physiological fluids, i.e. the metal bioaccessibility is driven by factors such as the solution chemical composition, the contact time with the particles, and the solid-to-liquid phase ratio (S/L). In this work, PM2.5-bound metal bioaccessibility was assessed in various physiological-like solutions including cell culture media in order to evidence the potential impact on normal human bronchial epithelial cells (NHBE) when studying the cytotoxicity and inflammatory responses of PM2.5 towards the target bronchial compartment. Different fluids (H2O, PBS, LHC-9 culture medium, Gamble and human respiratory mucus collected from COPD patients), various S/L conditions (from 1/6000 to 1/100,000) and exposure times (6, 24 and 72h) were tested on urban PM2.5 samples. In addition, metals' total, soluble and insoluble fractions from PM2.5 in LHC-9 were deposited on NHBE cells (BEAS-2B) to measure their cytotoxicity and inflammatory potential (i.e., G6PDH activity, secretion of IL-6 and IL-8). The bioaccessibility is solution-dependent. A higher salinity or organic content may increase or inhibit the bioaccessibiliy according to the element, as observed in the complex mucus matrix. Decreasing the S/L ratio also affect the bioaccessibility depending on the solution tested while the exposure time appears less critical. The LHC-9 culture medium appears to be a good physiological proxy as it induces metal bioaccessibilities close to the mucus values and is little affected by S/L ratios or exposure time. Only the insoluble fraction can be linked to the PM2.5-induced cytotoxicity. By contrast, both soluble and insoluble fractions can be related to the secretion of cytokines. The metal bioaccessibility in LHC-9 of the total, soluble, and insoluble fractions of the

  14. Components of executive control with advantages for bilingual children in two cultures.

    Science.gov (United States)

    Bialystok, Ellen; Viswanathan, Mythili

    2009-09-01

    The present study used a behavioral version of an anti-saccade task, called the 'faces task', developed by [Bialystok, E., Craik, F. I. M., & Ryan, J. (2006). Executive control in a modified anti-saccade task: Effects of aging and bilingualism. Journal of Experimental Psychology: Learning, Memory, and Cognition, 32, 1341-1354] to isolate the components of executive functioning responsible for previously reported differences between monolingual and bilingual children and to determine the generality of these differences by comparing bilinguals in two cultures. Three components of executive control were investigated: response suppression, inhibitory control, and cognitive flexibility. Ninety children, 8-years old, belonged to one of three groups: monolinguals in Canada, bilinguals in Canada, and bilinguals in India. The bilingual children in both settings were faster than monolinguals in conditions based on inhibitory control and cognitive flexibility but there was no significant difference between groups in response suppression or on a control condition that did not involve executive control. The children in the two bilingual groups performed equivalently to each other and differently from the monolinguals on all measures in which there were group differences, consistent with the interpretation that bilingualism is responsible for the enhanced executive control. These results contribute to understanding the mechanism responsible for the reported bilingual advantages by identifying the processes that are modified by bilingualism and establishing the generality of these findings across bilingual experiences. They also contribute to theoretical conceptions of the components of executive control and their development.

  15. Historical and Cultural Informativeness of French Phrasal Units with Component-dendronym

    Directory of Open Access Journals (Sweden)

    Taisiya I. Skorobogatova

    2016-12-01

    Full Text Available Research of national specificity of phraseology in a national language by distinguishing thematic groups of phraseological units and the analysis of their component composition becomes increasingly important in linguistic studies. This article is devoted to the analysis of the French phraseological units, which include dendronyms. Authors narrow notion of “dendronym” and use it only to refer to the names of trees. In French phraseological corpus authors identified 18 dendronyms, which are core components of the floral phrasal units: amandier (almond tree, cèdre (cedar, chêne (oak, cocotier (coconut palm, cyprès (cypress, figuier (fig tree, laurier (laurel tree, mûrier (mulberry, olivier (olive tree, orme (elm, osier (willow, palmier (palm, peuplier (poplar, platane (sycamore, poirier (pear, pommier (apple, prunier (plum, sapin (spruce, fir. The following list proves that the repertoire of dendronyms used to form French FU is not too wide. However, the authors confirmed the possibility to consider idioms-dendronym component as special items of historical and cultural memory.

  16. Effect of Sample Preparation on the Discrimination of Bacterial Isolates Cultured in Liquid Nutrient Media Using Laser-Induced Breakdown Spectroscopy (LIBS).

    Science.gov (United States)

    Gamble, Gary R; Park, Bosoon; Yoon, Seung-Chul; Lawrence, Kurt C

    2016-03-01

    Laser-induced breakdown spectroscopy (LIBS) is used as the basis for discrimination between two genera of gram-negative bacteria and two genera of gram-positive bacteria representing pathogenic threats commonly found in poultry processing rinse waters. Because LIBS-based discrimination relies primarily upon the relative proportions of inorganic cell components including Na, K, Mg, and Ca, this study aims to determine the effects of trace mineral content and pH found in the water source used to isolate the bacteria upon the reliability of the resulting discriminant analysis. All four genera were cultured using tryptic soy agar (TSA) as the nutrient medium, and were grown under identical environmental conditions. The only variable introduced is the source water used to isolate the cultured bacteria. Cultures of each bacterium were produced using deionized (DI) water under two atmosphere conditions, reverse osmosis (RO) water, tap water, phosphate buffered saline (PBS) water, and TRIS buffered water. After 3 days of culture growth, the bacteria were centrifuged and washed three times in the same water source. Bacteria were then freeze dried, mixed with microcrystalline cellulose, and a pellet was made for LIBS analysis. Principal component analysis (PCA) was used to extract related variations in LIBS spectral data among the four bacteria genera and six water types used to isolate the bacteria, and Mahalanobis discriminant analysis (MDA) was used for classification. Results indicate not only that the four genera can be discriminated from each other in each water type, but that each genus can be discriminated by water type used for isolation. It is concluded that in order for LIBS to be a reliable and repeatable method for discrimination of bacteria grown in liquid nutrient media, care must be taken to insure that the water source used in purification of the culture be precisely controlled regarding pH, ionic strength, and proportionate amounts of mineral cations

  17. Culture of reminiscing and the media narratives of the conflicts in Croatia

    Directory of Open Access Journals (Sweden)

    Jelena Vasiljević

    2016-03-01

    Full Text Available In this paper I am analyzing the use of memories, inscribing images of the past and speci- fic history interpretations present in Serbian and Croatian press published during the conflict in Croatia-period between 1991. and 1995. Although it is concerned with the media and the war in ex-Yugoslavia, the research presented here does not follow the known and many times formulated problem frame, which was conducted by all of these notions in order to de- monstrate their perilous co-dependence-through the role and responsibility of the media in the latest Balkan wars. What I am searching for in this paper are conceptions about war (and wider topics, such as, conflict and its causes. I am interested in the ways war conceptualized itself through the process of transformation/shaping the information from the battlefields into narratives, stories which created war reality, by offering explanatory frame, protagonists, motives, causes and purposes. In that sense, I see the press as an ideal source for studying the production of daily narratives regarding the war, since it reported daily on it, offering various models for elucidating the war and its interpretation, reflecting public perceptions that domi- nated the society. The analyzed material are texts from Serbian and Croatian press that covered the war in the mentioned period of five years-starting from the first war skirmishes in Plitvice up to Croatian "Storm" military operation. The essay is concerned with those texts that make images of the past topical and where collective memories appear as the shapers of the narratives, basis for understanding and self-understanding in the efforts of constructing a point of stability from which comprehending the reality would suggest/impose itself. From these texts we read different stories on war events which refer to history and pre-history in their explanations, bring suppressed emotions, old "truths" which were "proven" in the past and new ideas legitimized

  18. Application of principal component analysis-multivariate adaptive regression splines for the simultaneous spectrofluorimetric determination of dialkyltins in micellar media.

    Science.gov (United States)

    Ghasemi, Jahan B; Zolfonoun, Ehsan

    2013-11-01

    A new multicomponent analysis method, based on principal component analysis-multivariate adaptive regression splines (PC-MARS) is proposed for the determination of dialkyltin compounds. In Tween-20 micellar media, dimethyl and dibutyltin react with morin to give fluorescent complexes with the maximum emission peaks at 527 and 520nm, respectively. The spectrofluorimetric matrix data, before building the MARS models, were subjected to principal component analysis and decomposed to PC scores as starting points for the MARS algorithm. The algorithm classifies the calibration data into several groups, in each a regression line or hyperplane is fitted. Performances of the proposed methods were tested in term of root mean square errors of prediction (RMSEP), using synthetic solutions. The results show the strong potential of PC-MARS, as a multivariate calibration method, to be applied to spectral data for multicomponent determinations. The effect of different experimental parameters on the performance of the method were studied and discussed. The prediction capability of the proposed method compared with GC-MS method for determination of dimethyltin and/or dibutyltin. Copyright © 2013 Elsevier B.V. All rights reserved.

  19. The capacity of some newly bacteria and fungi for biodegradation of herbicide trifluralin under agiated culture media.

    Science.gov (United States)

    Erguven, G O; Bayhan, H; Ikizoglu, B; Kanat, G; Nuhoglu, Y

    2016-05-30

    Bioremediation is the use of microorganisms to degrade environmental contaminants (pesticides, polyaromatic hydrocarbons etc.) into less toxic forms or compounds. In this study microbial biodegradation of trifluralin was performed in liquid media with 11 different types of identified fungi and bacteria cultures and their mixtures in agiated culture media. The isolated fungi and bacteria mixtures showed the highest degradation, reaching 93% in the chemical oxygen demand (COD) parameter in four days and 82% as trifluralin active ingredient in five days. Bacteria and fungi mixtures achieved 69% and 66% degradations of trifluralin active ingredient respectively. In the fungi studies, the best removal was achieved by M.Chlamydosporia at 80%, in the bacteria studies, the best removal was achieved by Bacillus simplex about 95% in five days. These different removal rates were due to the microbial differencies.

  20. Medias miradas. Un análisis cultural de la imagen femenina (Enrique Gil Calvo

    Directory of Open Access Journals (Sweden)

    Mª Concepción Jimeno Calderón

    2008-05-01

    Full Text Available Enrique Gil Calvo nos ofrece un análisis de los signos visuales que se muestran en el escenario del anuncio publicitario como expresión de la identidad femenina. Para ello, a través de la observación de anuncios publicitarios impresos de distintas marcas de medias, como signo de distinción femenina (Medias Sely en 1933, Medias Eugenia de Montijo en 1960, Glory Complet en1964, Medias Balenciaga en 1966, Medias Helanca en 1980 estudia la repetición de los iconos y de los estereotipos utilizados, con una clara resonancia sexual (la mujer como objeto del deseo sexual masculino, como un transparente envoltorio de medias, dado el compulsivo fetichismo masculino y su dominio de la opinión pública.

  1. Biohydrogen production from rotten orange with immobilized mixed culture: Effect of immobilization media for various composition of substrates

    Energy Technology Data Exchange (ETDEWEB)

    Damayanti, Astrilia, E-mail: liasholehasd@gmail.com [Department of Chemical Engineering, Faculty of Engineering, Semarang State University, E1 Building, 2nd floor, Kampus Sekaran, Gunungpati, Semarang 50229 (Indonesia); Department of Chemical Engineering, Faculty of Engineering, Gadjah Mada University, Jl. Grafika No. 2, Kampus UGM, Yogyakarta 55281 (Indonesia); Sarto,; Syamsiah, Siti; Sediawan, Wahyudi B. [Department of Chemical Engineering, Faculty of Engineering, Gadjah Mada University, Jl. Grafika No. 2, Kampus UGM, Yogyakarta 55281 (Indonesia)

    2015-12-29

    Enriched–immobilized mixed culture was utilized to produce biohydrogen in mesophilic condition under anaerobic condition using rotten orange as substrate. The process was conducted in batch reactors for 100 hours. Microbial cultures from three different sources were subject to a series of enrichment and immobilized in two different types of media, i.e. calcium alginate (CA, 2%) and mixture of alginate and activated carbon (CAC, 1:1). The performance of immobilized culture in each media was tested for biohydrogen production using four different substrate compositions, namely orange meat (OM), orange meat added with peel (OMP), orange meat added with limonene (OML), and mixture of orange meat and peel added with limonene (OMPL). The results show that, with immobilized culture in CA, the variation of substrate composition gave significant effect on the production of biohydrogen. The highest production of biohydrogen was detected for substrate containing only orange meet, i.e. 2.5%, which was about 3-5 times higher than biohydrogen production from other compositions of substrate. The use of immobilized culture in CAC in general has increased the hydrogen production by 2-7 times depending on the composition of substrate, i.e. 5.4%, 4.8%, 5.1%, and 4.4% for OM, OMP, OML, and OMPL, respectively. The addition of activated carbon has eliminated the effect of inhibitory compounds in the substrate. The major soluble metabolites were acetic acid, propionic acid, and butyric acid.

  2. Biohydrogen production from rotten orange with immobilized mixed culture: Effect of immobilization media for various composition of substrates

    Science.gov (United States)

    Damayanti, Astrilia; Sarto, Syamsiah, Siti; Sediawan, Wahyudi B.

    2015-12-01

    Enriched-immobilized mixed culture was utilized to produce biohydrogen in mesophilic condition under anaerobic condition using rotten orange as substrate. The process was conducted in batch reactors for 100 hours. Microbial cultures from three different sources were subject to a series of enrichment and immobilized in two different types of media, i.e. calcium alginate (CA, 2%) and mixture of alginate and activated carbon (CAC, 1:1). The performance of immobilized culture in each media was tested for biohydrogen production using four different substrate compositions, namely orange meat (OM), orange meat added with peel (OMP), orange meat added with limonene (OML), and mixture of orange meat and peel added with limonene (OMPL). The results show that, with immobilized culture in CA, the variation of substrate composition gave significant effect on the production of biohydrogen. The highest production of biohydrogen was detected for substrate containing only orange meet, i.e. 2.5%, which was about 3-5 times higher than biohydrogen production from other compositions of substrate. The use of immobilized culture in CAC in general has increased the hydrogen production by 2-7 times depending on the composition of substrate, i.e. 5.4%, 4.8%, 5.1%, and 4.4% for OM, OMP, OML, and OMPL, respectively. The addition of activated carbon has eliminated the effect of inhibitory compounds in the substrate. The major soluble metabolites were acetic acid, propionic acid, and butyric acid.

  3. The preparation and culture of washed human sperm:A comparison of a suite of protein-free media with media containing human serum albumin

    Institute of Scientific and Technical Information of China (English)

    Kelli L Peirce; Peter Roberts; Jaffar Ali; Phillip Matson

    2015-01-01

    Objective:To compare two suites of culture media (one with HSA and one protein-free (PF) supplemented with methylcellulose) for washing human sperm in IVF.Methods:Semen samples (n=41) underwent parallel density gradient preparation using PF or HSA-supplemented culture medium and subsequent yield, survival, morphology and motility were compared.Results:The PF medium resulted in a significantly higher sperm yield (P<0.0001), but similar sperm morphology (P=0.822) and 24-hour survival (P=0.11). There was, however, a lower percentage of progressively motile sperm (P<0.0001) and a higher proportion of sperm demonstrating non-progressive motility (P<0.0001) in the PF medium when observed on a Makler Chamber, apparently an artefact as a similar sperm motility index was measured using a Sperm Quality Analyser (P=0.83). Attachment of sperm in PF medium to the glass chamber reduced with time and any differences had disappeared after 6 minutes on the counting chamber.Conclusion:These results support the use of PF media supplemented with methylcellulose as an alternative to HSA, although a modification to the manufacturer’s protocol of 6-minutes pre-incubation before assessing sperm motility must be used. Further studies should investigate the function of such sperm prepared in PF medium.

  4. Socio Cultural Media Sharing as Conversations Sensing and Modeling Behavior in Response to Environmental Changes

    Science.gov (United States)

    2015-07-06

    media systems and online social networks . Media Coverage vs. Online Discussion: We aim to compare the public opinions of specific topics like “Gun...frequently are messages from users with larger networks and whose tweets contain hashtags. Results also show a significant negative relationship between the...considering the context of evolving mass media systems and online social networks . Distribution Statement This is block 12 on the SF298 form

  5. MediaCommons for cultural heritage: Applied mixed media visualization storytelling for high resolution collaborative cyberarchaeological displays

    KAUST Repository

    Mangan, John

    2013-10-01

    Archaeology is a discipline that studies time through an understanding of space and objects in that space; archaeology is ultimately, therefore, an intersection where the visualization of space and the visualization of time meet. Archaeology has long utilized visualization as a technique to analyze and disseminate information; however, comprehensive and collaborative analysis and storytelling with this visual data has always been limited by the capacity of the systems, which create and display it. To present the most complete narrative of the past, one must seek the \\'big picture\\' by assembling the disparate pieces of data, which reflect the lives of the humans we study. This paper presents a framework for the visualization of and interaction with rich data collections in high resolution, networked, tiled-display environments, called the MediaCommons Framework. © 2013 IEEE.

  6. Community Media: Muting the Democratic Media Discourse?

    NARCIS (Netherlands)

    Carpentier, N.; Lie, R.; Servaes, J.

    2003-01-01

    Focuses on the concept of community media. Components that construct the identity of community media; Multi-theoretical approaches for analysis of community media; Definition of community media based on the concept of alternative media; Link between community media and civil society; Problems faced

  7. Effects of Culture Media and Light Intensity on in vitro Growth of Oncidium under CO2 Enrichment Condition

    Institute of Scientific and Technical Information of China (English)

    He Songlin; Pan Huitang; Yang Qiusheng; Kong Dezheng; Zhang Qixiang; Michio Tanaka

    2003-01-01

    The effects of culture media and light intensity on in vitro growth of Oncidium 'Aloha Iwanga' were investigated under CO2 enrichment condition. Height, fresh and dry weight of the Oncidium seedlings were measured, and the leaf number per plant, shoot number per plant, leaf width and leaf chlorophyll content were also investigated. The results were as follows: 1) The seedling height, fresh and dry weight, leaf number per plant, leaf width and leaf chlorophyll content of the shoots growing on MS complete culture medium were higher than those on 1/2MS, VW and 1/2VW media. The root number per plant and ratio of dry matter of the seedlings cultured on 1/2MS and 1/2VW media were higher than those on MS and VW; 2) The seedling height, fresh weight, dry weight, dry matter ratio and leaf chlorophyll content, leaf length, leaf width, root length, leaf number per plant, root number per plant of seedlings of Oncidium growing under 4 500 lx and 1 700 lx were higher than those under 750 lx. However, there was no significant difference in those growth parameters mentioned above while dealing with 4 500 lx and 1 700 lx except for the seedling height. Nevertheless, the leaf color of plants under 4 500 lx was lighter and the leaves of the lower parts became yellowish in comparison with those growing under 1 700 lx.

  8. Tunable swelling of polyelectrolyte multilayers in cell culture media for modulating NIH-3T3 cells adhesion.

    Science.gov (United States)

    Qi, Wei; Cai, Peng; Yuan, Wenjing; Wang, Hua

    2014-11-01

    For polyelectrolyte multilayers (PEMs) assembled by the layer-by-layer (LbL) assembly technique, their nanostructure and properties can be governed by many parameters during the building process. Here, it was demonstrated that the swelling of the PEMs containing poly(diallyldimethylammonium chloride) (PDDA) and poly(sodium 4-styrenesulfonate) (PSS) in cell culture media could be tuned with changing supporting salt solutions during the assembly process. Importantly, the influence of the PEMs assembled in different salt solutions on NIH-3T3 cell adhesion was observable. Specifically, the cells could possess a higher affinity for the films assembled in low salt concentration (i.e. 0.15M NaCl) or no salt, the poorly swelling films in cell culture media, which was manifested by the large cell spreading area and focal adhesions. In contrast, those were assembled in higher salt concentration, highly swelling films in cell culture media, were less attractive for the fibroblasts. As a result, the cell adhesion behaviors may be manipulated by tailoring the physicochemical properties of the films, which could be performed by changing the assembly conditions such as supporting salt concentration. Such a finding might promise a great potential in designing desired biomaterials for tissue engineering and regenerative medicine.

  9. Effect of active species on animal cells in culture media induced by DBD Plasma irradiation using air

    Science.gov (United States)

    Ohtsubo, Tetsuya; Ono, Reoto; Hayashi, Nobuya

    2015-09-01

    Little has been reported on action mechanism of active species produced by plasmas affecting living cells. In this study, active species in culture medium generated by torch type DBD and variations of animal cells are attempted to be clarified. Animal cells are irradiated by DBD plasma through various media such as DMEM, PBS and distilled water. Irradiation period is 1 to 15 min. The distance between the lower tip of plasma touch and the surface of the medium is 10 mm. Concentrations of NO2 -, O2 in liquid are measured. After the irradiation, the cells were cultivated in culture medium and their modifications are observed by microscope and some chemical reagents. Concentration of NO2 - and H2 O2 in all media increased with discharge period. Increase rate of NO2 -concentration is much higher than that of hydrogen peroxide. After plasma irradiation for 15 min, concentrations of NO2 were 80 mg/L in DMEM, 30 mg/L in PBS and 15 mg/L in distilled water. Also, the concentration of H2 O2 became 3mg/L in DMEM, 6.5 mg/L in PBS and 6.5mg/L in distilled water. The significant inactivation of cells was observed in the PBS. Above results indicate that, in this experiment, H2 O2 or OH radicals would affect animal cells in culture media.

  10. Identification of Candida albicans by using different culture medias and its association in potentially malignant and malignant lesions

    Directory of Open Access Journals (Sweden)

    Sonal Saigal

    2011-01-01

    Full Text Available Background and Objective: The present study evaluates the association of Candida albicans with normal control group, potentially malignant and malignant lesions of oral cavity by using two different liquid culture media. Materials and Methods: Saliva was collected and biopsy was taken only from those clinically suspected potentially malignant and malignant lesions for histopathological diagnosis. Saliva samples were inoculated for fungal growth in Sabouraud′s dextrose agar and culture-positive samples had undergone for Germ tube test. Germ tube-positive samples were further taken for quantification of chlamydospore production in liquid media at 8 and 16 hours. Results: In normal control groups no fungus growth was found; however, potentially malignant and malignant cases showed fungus growth, positive germ tube test and chlamydospore formation. The result also showed rapid and quantitatively more chlamydospore formation in corn meal broth + 5% milk in comparison to serum milk culture media. Conclusion: The oral mucosa is compromised in potentially malignant lesions, it can be argued that this species may be involved in carcinogenesis by elaborating the nitrosamine compounds which either act directly on oral mucosa or interact with other chemical carcinogens to activate specific proto-oncogenes and thereby initiate oral neoplasia.

  11. EDUCATION AS A SOCIO-CULTURAL PHENOMENON: ANALYSIS OF VALUETARGET COMPONENTS

    Directory of Open Access Journals (Sweden)

    Victor A. SHTURBA

    2015-01-01

    Full Text Available In the article authors analyze the socio-cultural phenomenon of education through the prism of its values and target components. Understanding of the role and place of education in society is not possible out of the context the human essence. In living nature man appears as a phenomenon - this thesis is recognized the absolute majority of scientific and philosophical theories, concepts and doctrines, including nonhumanistic oriented. It is known that man is a social being - society is environment for its full life. As Aristotle said, only the gods and the animals can live outside society. However, human nature does not imply a simple social existence in the form of an intensive exchange of information with similar entities and, based on this, building complex collective actions. The man has a mind that is capable of abstract thought, speech, language, complex psyche, important component of which is imagination. These features formed the basis of theoretical and methodological basis of the analysis of education as a phenomenon. The authors indicated value and target measurement of socio-cultural phenomenon of education, which led to the conclusion about the need to rely on a properly organized philosophical and pedagogical knowledge, the importance of implementation in the educational process of program-target approach and the relevance of the audit of modern educational models for nonborrowed trainings, upbringing and socialization.

  12. Antagonism among Gluconacetobacter diazotrophicus strains in culture media and in endophytic association.

    Science.gov (United States)

    Muñoz-Rojas, Jesús; Fuentes-Ramírez, Luis E; Caballero-Mellado, Jesús

    2005-09-01

    In this study the antagonistic activity among 55 Gluconacetobacter diazotrophicus strains, belonging to 13 electrophoretic types (ETs), in culture media was analyzed. Antagonistic effects were seen only in strains belonging to two ETs named ET-1 and ET-3. Two out of 29 ET-1 strains, and 3 out of 7 ET-3 strains of G. diazotrophicus showed antagonistic effects against many other strains belonging to all the ETs of this species analyzed, and against closely related strains of Gluconacetobacter species, including Gluconacetobacter johannae, Gluconacetobacter azotocaptans and Gluconacetobacter liquefaciens but not against other phylogenetically distant bacterial species. Results showed that the substance responsible of such antagonistic activity is a low molecular mass molecule (approximately 3400 Da), stable from pH 3.5 to 8.5, and very stable at 4 degrees C for 10 months. This substance was sensitive to proteases, and the antagonistic activity was lost after 2 h at 95 degrees C. All of these features show that the substance is related to bacteriocin-like molecules. The antagonistic substance should be chromosomally encoded because ET-3 strains of G. diazotrophicus do not harbor any plasmids. The antagonistic ability of ET-3 strains of G. diazotrophicus could be an advantage for the natural colonization of the sugarcane environment, as was observed in experiments with micropropagated sterile sugarcane plantlets co-inoculated with a bacteriocin-producer strain and a bacteriocin-sensitive strain of G. diazotrophicus. In these experiments, both in the rhizosphere as well as inside the roots, the bacteriocin-sensitive population decreased drastically. In addition, this study shows that inside the plants there may exist antagonistic interactions among endophytic bacteria like to those described among the rhizospheric community.

  13. 媒介文化:一种废弃论分析%Media Culture:Analysis of Waste Theory

    Institute of Scientific and Technical Information of China (English)

    潘天波; 刘永宁

    2014-01-01

    在传媒时代,现代媒介已然开启知识的生产、消费与接受的新路径。现代性进程既是书写发展、进步与增益的文明史,也是书写过度、剩余、废弃物以及废弃物处理的荒野史。大规模的媒介文化生产也意味着废弃主义时代的来临,媒介技术缺陷、传播主观色彩、讯息光缆拥塞与他择性传播接受等都是媒介废弃物生产的致命隐患。对现代媒介的废弃论分析,旨在检阅与反思现代传媒及其背后的文化状态,以期诊断媒介文化的废弃假象以及救赎我们对媒介文化的理解力。%In the media era,the modern media have opened the door to a new path of knowledge production, consumption and accept.The process of modernity is not only to write a civilization history of development, progress and gains,but also make out a wilderness history of immoderacy,surplus,waste and waste han-dling.Mass media culture production also means the arrival of the rejecting era.Technical defects,commu-nication subj ective color,message transportation congestion and alternative spread and accept are fatal hid-den trouble of media waste production.Analysis of the modern media waste theory aims to review and re-flect on modern communication and the media culture,in order to diagnose and save our understanding of the media culture.

  14. Best practices for the use and evaluation of animal serum as a component of cell culture medium.

    Science.gov (United States)

    Nims, Raymond W; Harbell, John W

    2017-07-21

    Animal serum is a common additive for cell culture medium and is often required at 5 to 10% (v/v) for the attachment and growth of primary and continuous anchorage-dependent (monolayer) cultures. The use of animal serum in cell culture medium confers several advantages and also some risks. This article discusses the use of animal serum as a component of cell culture medium. The best practices associated with the sourcing, storage, thawing, testing, and mitigation of risk associated with the use of animal sera are among the topics described in this article.

  15. Immigrant Hungarian Families' Perceptions of New Media Technologies in the Transmission of Heritage Language and Culture

    Science.gov (United States)

    Szecsi, Tunde; Szilagyi, Janka

    2012-01-01

    The goal of this research was to explore thoroughly the perceptions of dispersed immigrant professionals and their bilingual and bicultural children regarding the place of new media technology in their lives. Open-ended interviewing and autoethnography were used to explore families' perceptions of the role of media technology in their children's…

  16. From Snow White to Digimon: Using Popular Media to Confront Confucian Values in Taiwanese Peer Cultures

    Science.gov (United States)

    Hadley, Kathryn Gold; Nenga, Sandi Kawecka

    2004-01-01

    Parents, educators and social commentators have repeatedly claimed that passive media consumption can harm children. Building on recent attempts to understand how children actively interpret media, the authors use an interpretive model of socialization to analyze fieldnote excerpts from a Taiwanese kindergarten and first grade. Contrary to popular…

  17. Media Literacy in the ESL/ESL Classroom: Reading Images and Cultural Stories.

    Science.gov (United States)

    Quinlisk, Carla Chamberlin

    2003-01-01

    Stresses the importance of developing media literacy in the English-as-a-Second/Foreign-Language classroom, not only as a way to incorporate mass media as a source of linguistic input, but also as a tool for learning to interpret multiple layers of messages and to separate mediated images of people, places, things, ideas, and values from those of…

  18. Qualitative process evaluation of an Australian alcohol media literacy study: recommendations for designing culturally responsive school-based programs.

    Science.gov (United States)

    Gordon, Chloe S; Kervin, Lisa K; Jones, Sandra C; Howard, Steven J

    2017-02-02

    Alcohol media literacy programs seek to mitigate the potentially harmful effects of alcohol advertising on children's drinking intentions and behaviours through equipping them with skills to challenge media messages. In order for such programs to be effective, the teaching and learning experiences must be tailored to their specific cultural context. Media in the Spotlight is an alcohol media literacy program aimed at 9 to 12 year old Australian children. This study evaluates the process and implementation of the program, outlining the factors that facilitated and inhibited implementation. From this evaluation, a pedagogical framework has been developed for health professionals implementing culturally responsive programs in school settings. Process measures included: semi-structured interviews with teachers before and after the program was implemented (n = 11 interviews), program evaluation questionnaires completed by children (n = 166), lesson observations completed by teachers (n = 35 observations), and reflective journal entries completed by the researcher (n = 44 entries). A thematic analysis approach was used to analyse all of the data sets using NVivo. Inductive coding was used, whereby the findings were derived from the research objectives and multiple readings and interpretations of the data. Five key pedagogical considerations were identified that facilitated implementation. These were: connecting to the students' life worlds to achieve cultural significance; empowering students with real-world skills to ensure relevance; ensuring programs are well structured with strong connections to the school curriculum; creating developmentally appropriate activities while providing a range of assessment opportunities; and including hands-on and interactive activities to promote student engagement. Three potential inhibitors to implementing the alcohol media literacy program in upper-elementary school classrooms were identified. These included topic

  19. Polymerase Chain Reaction (PCR) Versus Bacterial Culture in Detection of Organisms in Otitis Media with Effusion (OME) in Children.

    Science.gov (United States)

    Aly, Balegh H; Hamad, Mostafa S; Mohey, Mervat; Amen, Sameh

    2012-03-01

    The aim of this study was to compare between polymerase chain reaction (PCR) and bacterial culture in detection of Streptococcus Pneumonia and M. Catarrhalis in otitis media with effusion (OME) in children. Fifty patients having OME were included in this study between 2003 and 2008. Myringotomy and tympanostomy tube insertion were done in every patient and the middle ear effusion samples were aspirated. The samples were subjected to bacteriological study in the form of culture and molecular study in the form of PCR using JM201/202-204 primer probe set for both S. pneumonia and M. catarrhalis. The results of Bacterial cultures are as follows: five cases (10%) were culture positive for S. pneumonia. Six cases (12%) were culture positive for M. catarrhalis. Only one case (2%) showed positively for both S. pneumonia and M. catarrhalis. Polymerase chain reaction test shows that 18 cases (36%) were positive for S. pneumonia, 22 cases (44%) were positive for M. catarrhalis, 6 cases (12%) were positive for both organism and 4 cases (8%) were negative. The difference between the proportion of culture positive and PCR positive specimens for both organisms individually and collectively was significant (P PCR is more accurate than bacterial culture in detection of organisms in middle ear fluid in OME and that M. catarrhalis plays a significant rule in OME as it is the sole organism identified more than the other one by PCR.

  20. Application of stem-cell media to explant culture of human periosteum: An optimal approach for preparing osteogenic cell material

    Directory of Open Access Journals (Sweden)

    Kohya Uematsu

    2013-10-01

    Full Text Available As part of our clinical tests on bone regeneration using cultured periosteal sheets, here, we prepared cultured periosteal sheets in two types of stem-cell culture media, STK1 and STK3. Human periosteum was expanded either in 1% human serum–supplemented STK1 for 28 days, in 1% human serum–supplemented STK1 for 14 days followed by 1% human serum–supplemented STK3 for 14 days (1% human serum–supplemented STK1+3, or in 10% fetal bovine serum–supplemented Medium 199 for 28 days (control. Cultured periosteal sheet diameter and DNA content were significantly higher, and the multilayer structure was prominent in 1% human serum–supplemented STK1 and 1% human serum–supplemented STK1+3. The messenger RNA of osteoblastic markers was significantly upregulated in 1% human serum–supplemented STK1+3. Osteopontin-immunopositive staining and mineralization were evident across a wide area of the cultured periosteal sheet in 1% human serum–supplemented STK1+3. Subcutaneous implantation in nude mice following expansion in 1% human serum–supplemented STK1+3 produced the highest cultured periosteal sheet osteogenic activity. Expansion in 1% human serum–supplemented STK1+3 successfully induced cultured periosteal sheet growth while retaining osteogenic potential, and subsequent osteoblastic induction promoted the production of homogeneous cell material.

  1. Identification and quantitative determination of pinoresinol in Taxus ×media Rehder needles, cell suspension and shoot cultures

    Directory of Open Access Journals (Sweden)

    Paulina Mistrzak

    2015-01-01

    Full Text Available The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of Taxus ×media. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of T. ×media. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw was about twice as high as that of free pinoresinol (0.43 mg/g dw. The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.

  2. Use of Selective Fungal Culture Media Increases Rates of Detection of Fungi in the Respiratory Tract of Cystic Fibrosis Patients.

    Science.gov (United States)

    Hong, Gina; Miller, Heather B; Allgood, Sarah; Lee, Richard; Lechtzin, Noah; Zhang, Sean X

    2017-04-01

    The prevalence of fungi in the respiratory tracts of cystic fibrosis (CF) patients has risen. However, fungal surveillance is not routinely performed in most clinical centers in the United States, which may lead to an underestimation of the true prevalence of the problem. We conducted a prospective study comparing the rates of detection for clinically important fungi (CIF), defined as Aspergillus, Scedosporium, and Trichosporon species and Exophiala dermatitidis, in CF sputa using standard bacterial and selective fungal culture media, including Sabouraud dextrose agar with gentamicin (SDA), inhibitory mold agar (IMA), and brain heart infusion (BHI) agar with chloramphenicol and gentamicin. We described the prevalence of these fungi in an adult CF population. A total of 487 CF respiratory samples were collected from 211 unique participants. CIF were detected in 184 (37.8%) samples. Only 26.1% of CIF-positive samples were detected in bacterial culture medium, whereas greater rates of detection for fungi were found in IMA (65.8%; P fungi by bacterial culture alone. The prevalence of fungi in CF may be better estimated by using selective fungal culture media, and this may translate to important clinical decisions. Copyright © 2017 American Society for Microbiology.

  3. Movie-An Important Media in Cultural Communication between China and America

    Institute of Scientific and Technical Information of China (English)

    信丹丹

    2014-01-01

    Culture is the accumulation of life style and idea. As the container of culture, movies become the most important me⁃dia of cross-cultural communication. This paper explains movie’s significance for cross-cultural communication, it tells of the in⁃ter-effects on life and spirit between Chinese and American, it provides the strategy for cross-cultural communication for movies.

  4. Implementing high-temperature short-time media treatment in commercial-scale cell culture manufacturing processes.

    Science.gov (United States)

    Pohlscheidt, Michael; Charaniya, Salim; Kulenovic, Fikret; Corrales, Mahalia; Shiratori, Masaru; Bourret, Justin; Meier, Steven; Fallon, Eric; Kiss, Robert

    2014-04-01

    The production of therapeutic proteins by mammalian cell culture is complex and sets high requirements for process, facility, and equipment design, as well as rigorous regulatory and quality standards. One particular point of concern and significant risk to supply chain is the susceptibility to contamination such as bacteria, fungi, mycoplasma, and viruses. Several technologies have been developed to create barriers for these agents to enter the process, e.g. filtration, UV inactivation, and temperature inactivation. However, if not implemented during development of the manufacturing process, these types of process changes can have significant impact on process performance if not managed appropriately. This article describes the implementation of the high-temperature short-time (HTST) treatment of cell culture media as an additional safety barrier against adventitious agents during the transfer of a large-scale commercial cell culture manufacturing process. The necessary steps and experiments, as well as subsequent results during qualification runs and routine manufacturing, are shown.

  5. An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms

    Energy Technology Data Exchange (ETDEWEB)

    Holmes, Aimee E.; Sego, Landon H.; Webb-Robertson, Bobbie-Jo M.; Kreuzer, Helen W.; Anderson, Richard M.; Unwin, Stephen D.; Weimar, Mark R.; Tardiff, Mark F.; Corley, Courtney D.

    2013-02-01

    We demonstrate an approach for assessing the quality of a signature system designed to predict the culture medium used to grow a microorganism. The system was comprised of four chemical assays designed to identify various ingredients that could be used to produce the culture medium. The analytical measurements resulting from any combination of these four assays can be used in a Bayesian network to predict the probabilities that the microorganism was grown using one of eleven culture media. We evaluated combinations of the signature system by removing one or more of the assays from the Bayes network. We measured and compared the quality of the various Bayes nets in terms of fidelity, cost, risk, and utility, a method we refer to as Signature Quality Metrics

  6. In vitro culture of Cucumis sativus L. VI. Histological analysis of leaf explants cultured on media with 2, 4-D or 2, 4, 5-T

    Directory of Open Access Journals (Sweden)

    Anna Nadolska-Orczyk

    2014-01-01

    Full Text Available The developmental sequence of callus initiation and somatic embryogenesis in leaf explants of Cucumis sativus cv. Borszczagowski was analysed and compared on media containing two different auxin phenoxy-derivatives (2,4-D and 2,4,5-T and cytokinin (BAP or 2iP. During the first 20 days of culture on media with 2,4,5-T proliferation of parenchymatic tissue occurred mainly and only small meristematic centers were observed. There was an intensive detachment of parenchymatic cells and dissociation of their cell walls near vessels and in the lower part of the explant adjacent to the medium. These cells were strongly plasmolysed. On the 2,4-D containing medium mostly meristematic tissue developed, proliferating around vascular bundles and forming meristematic centers or promeristem-like structures. After 35-50 days of culture, secondary callus was formed by separation of meristematic cells from the meristem surface in explants cultured on the 2,4-D containing medium. On medium supplemented with 2, 4, 5-T the detachment of parenchymatic and meristematic cells occurred, along with formation of a gel-like substance. The gel-like callus contained multi-cellular aggregates, proembryoids and embryoids. This type of callus tissue was initiated more intensively on medium with 2, 4, 5-T, but the frequency of somatic embryogenesis was much lower. The periferial cells of aggregates, proembryoids and embryoids showed the tendency to separate from the surface of the tissue. Many embryoids formed adventitious embryos.

  7. [Classification of anticancer drugs with different mechanisms based on amino-acid consumption profiling in culture media].

    Science.gov (United States)

    Han, Xiaofei; Wang, Longxing; Yang, Qianxu; Xiao, Hongbin

    2011-04-01

    An approach for quantitative determination of amino-acid consumption profiling in culture media by high performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed and validated, using o-phthalic dicarboxaldehyde (OPA) as the derivatizing reagent and norvaline as the internal standard. Mobile phase A was 10 mmol/L Na2HPO4Na2B4O7 buffer (pH 7.95), and mobile phase B was acetonitrile-methanol-water (45:45:10, v/v/v). The linear elution program was 5% B at the start and 52% B at the end in 35 min. The 17 free amino-acids (FAAs) were separated satisfactorily in 33 min. Following HeLa cells incubation in conditioned medias of taxol (4 micromol/L) and mitomycin (75 micromol/L), respectively, with control for 24 h, the media 17 amino-acid consumption profilings were determined, and then analyzed by multivariate statistical analysis based on Matlab7.1 software platform. Relation analysis performed by partial least squares-discriminant analysis (PLS-DA) indicated that in comparison with the control group, the media amino-acid consumption profiling can distinguish the two anticancer drugs with different mechanisms, which provides a new perspective for the pre-classification of drug action mechanisms during the screening of new anticancer drugs. Meanwhile, the idea from the outer into the inner has convenient and economic characteristics.

  8. The Investigation of the effect of Emotional Intelligence and Spiritual Intelligence Components on the Organizational Culture of Ilam University Staff

    Directory of Open Access Journals (Sweden)

    Seidmehdi Mehdi Veiseh

    2014-12-01

    Full Text Available Spiritual intelligence has been defined as a framework for identifying and organizing skills and competencies necessary to use adaptive spirituality. The present study seeks to investigate the effect of the components of emotional intelligence and spiritual intelligence on the organizational culture of staff of Ilam University. The population under study was the staff of Ilam University, who was 400 people. The sample size was 110, which were randomly selected. To collect the data, Bar-on Emotional Intelligence (EQ scale and Amram and Dryer‟s Integrated Spiritual Intelligences Scale (ISIS were used. The obtained data were then analyze dusing Pearson correlation and simultaneous multiple regression analysis. The results showed that there is a significant relationship between emotional intelligence and organizational culture, also spiritual intelligence and organizational culture. The results also revealed that among the components of emotional intelligence and spiritual intelligence, intrapersonal skills, interpersonal skills, adaptability, Transcendence, dignity and Meaningful are significant predictors for organization culture, respectively.

  9. Social media and pre-project inter-cultural dialogue in international workcamps: potential and reality

    OpenAIRE

    Burghardt, Anne

    2015-01-01

    Vor dem Hintergrund ihrer weltweiten Popularität haben einige Praktiker der internationalen Jugendarbeit die Vermutung geäußert, Teilnehmende internationaler Begegnungsmaßnahmen würden, ohne Zutun der Teamer, bereits vor Beginn einer Begegnung Social Media in interkulturellen Dialog treten. Aufgrund dieser Annahme wurde eine empirische Studie durchgeführt, welche Aufschluss darüber gibt, ob und wie Teilnehmende internationaler Workcamps Social Media wirklich vor Beginn eines Projektes als Kom...

  10. Bioenergetics of the growth of the lactobacillus culture on various nutrient media

    Science.gov (United States)

    Ur'yash, V. F.; Gorlova, I. S.; Novoselova, N. V.; Kon'kova, N. K.

    2010-07-01

    The energy (enthalpy) of the multiplication of lactobacilli on various nutrient media was measured on a DAK-1-1 differential microcalorimeter. Cultivation of lactobacilli on the nutrient media studied was accompanied by a release of energy. The time dependences of heat evolution were similar in shape to the curves of the growth of the microorganism population. The conclusion was drawn that the quantity of the evolved energy depended on the type of the nutrient medium.

  11. New Media Streaming and Cultural Development in Ethnic Minority Areas%新兴媒体流与少数民族地区文化发展

    Institute of Scientific and Technical Information of China (English)

    马小凤

    2011-01-01

    文章在分析新媒体的发展现状和新媒体在少数民族地区发展的文化背景的基础上,探讨了新媒体与少数民族文化价值的关系.%The paper analyzes the current development of new media and the cultural background of new media in the development of ethnic minority areas, on the basis, explores the relations between new media and cultural values of ethnic.

  12. Individual and culture-level components of survey response styles: A multi-level analysis using cultural models of selfhood.

    Science.gov (United States)

    Smith, Peter B; Vignoles, Vivian L; Becker, Maja; Owe, Ellinor; Easterbrook, Matthew J; Brown, Rupert; Bourguignon, David; Garðarsdóttir, Ragna B; Kreuzbauer, Robert; Cendales Ayala, Boris; Yuki, Masaki; Zhang, Jianxin; Lv, Shaobo; Chobthamkit, Phatthanakit; Jaafar, Jas Laile; Fischer, Ronald; Milfont, Taciano L; Gavreliuc, Alin; Baguma, Peter; Bond, Michael Harris; Martin, Mariana; Gausel, Nicolay; Schwartz, Seth J; Des Rosiers, Sabrina E; Tatarko, Alexander; González, Roberto; Didier, Nicolas; Carrasco, Diego; Lay, Siugmin; Nizharadze, George; Torres, Ana; Camino, Leoncio; Abuhamdeh, Sami; Macapagal, Ma Elizabeth J; Koller, Silvia H; Herman, Ginette; Courtois, Marie; Fritsche, Immo; Espinosa, Agustín; Villamar, Juan A; Regalia, Camillo; Manzi, Claudia; Brambilla, Maria; Zinkeng, Martina; Jalal, Baland; Kusdil, Ersin; Amponsah, Benjamin; Çağlar, Selinay; Mekonnen, Kassahun Habtamu; Möller, Bettina; Zhang, Xiao; Schweiger Gallo, Inge; Prieto Gil, Paula; Lorente Clemares, Raquel; Campara, Gabriella; Aldhafri, Said; Fülöp, Márta; Pyszczynski, Tom; Kesebir, Pelin; Harb, Charles

    2016-12-01

    Variations in acquiescence and extremity pose substantial threats to the validity of cross-cultural research that relies on survey methods. Individual and cultural correlates of response styles when using 2 contrasting types of response mode were investigated, drawing on data from 55 cultural groups across 33 nations. Using 7 dimensions of self-other relatedness that have often been confounded within the broader distinction between independence and interdependence, our analysis yields more specific understandings of both individual- and culture-level variations in response style. When using a Likert-scale response format, acquiescence is strongest among individuals seeing themselves as similar to others, and where cultural models of selfhood favour harmony, similarity with others and receptiveness to influence. However, when using Schwartz's (2007) portrait-comparison response procedure, acquiescence is strongest among individuals seeing themselves as self-reliant but also connected to others, and where cultural models of selfhood favour self-reliance and self-consistency. Extreme responding varies less between the two types of response modes, and is most prevalent among individuals seeing themselves as self-reliant, and in cultures favouring self-reliance. As both types of response mode elicit distinctive styles of response, it remains important to estimate and control for style effects to ensure valid comparisons. © 2016 International Union of Psychological Science.

  13. Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography.

    Science.gov (United States)

    Fa, Yun; Yang, Haiyan; Ji, Chengshuai; Cui, He; Zhu, Xinshu; Du, Juan; Gao, Jun

    2013-10-10

    An improved method for the simultaneous determination of 20 amino acids and 7 carbohydrates using one-valve switching after injection, ion chromatography, and integrated pulsed amperometric detection is proposed. The resolution of the amino acids and carbohydrates in the cation trap column was investigated. In addition, parameters including flow liquid type, flow rate, concentration, and valve-switch timing were optimized. The method is time-saving, effective, and accurate for the simultaneous separation of amino acids and carbohydrates, with a mean correlation coefficient of >0.99 and repeatability of 0.5-4.6% for eight replicates. The method was successfully applied in the analysis of amino acids and carbohydrates in aseptic media and in extracellular culture media of three phenotypes of Clostridium thermocellum.

  14. Bimbo or boffin? Women in science: an analysis of media representations and how female scientists negotiate cultural contradictions.

    Science.gov (United States)

    Chimba, Mwenya; Kitzinger, Jenny

    2010-09-01

    This paper examines the gendered representations of scientists in the UK media. Our analysis reveals the asymmetrical ways in which men and women working in science, engineering and technology are portrayed, in particular through the emphasis on women's appearance and a focus on their exceptional status. It also highlights the way female scientists may be used to "sex up" the discipline in the context of increasing concern about the (un)popularity of science. This media analysis is contextualized by drawing on data from 86 scientists examining how women themselves experience press and television coverage and address the cultural contradictions surrounding their role. The research highlights the challenges facing women working in these fields and the dilemmas for those seeking to develop a "human" face for science and promote a positive image for women.

  15. Exact Solution for Non-Self-Similar Wave-Interaction Problem during Two-Phase Four-Component Flow in Porous Media

    Directory of Open Access Journals (Sweden)

    S. Borazjani

    2014-01-01

    Full Text Available Analytical solutions for one-dimensional two-phase multicomponent flows in porous media describe processes of enhanced oil recovery, environmental flows of waste disposal, and contaminant propagation in subterranean reservoirs and water management in aquifers. We derive the exact solution for 3×3 hyperbolic system of conservation laws that corresponds to two-phase four-component flow in porous media where sorption of the third component depends on its own concentration in water and also on the fourth component concentration. Using the potential function as an independent variable instead of time allows splitting the initial system to 2×2 system for concentrations and one scalar hyperbolic equation for phase saturation, which allows for full integration of non-self-similar problem with wave interactions.

  16. CD109 is a component of exosome secreted from cultured cells.

    Science.gov (United States)

    Sakakura, Hiroki; Mii, Shinji; Hagiwara, Sumitaka; Kato, Takuya; Yamamoto, Noriyuki; Hibi, Hideharu; Takahashi, Masahide; Murakumo, Yoshiki

    2016-01-22

    Exosomes are 50-100-nm-diameter membrane vesicles released from various types of cells. Exosomes retain proteins, mRNAs and miRNAs, which can be transported to surrounding cells. CD109 is a glycosylphosphatidylinositol-anchored glycoprotein, and is released from the cell surface to the culture medium in vitro. Recently, it was reported that secreted CD109 from the cell surface downregulates transforming growth factor-β signaling in human keratinocytes. In this study, we revealed that CD109 is a component of the exosome in conditioned medium. FLAG-tagged human CD109 (FLAG-CD109) in conditioned medium secreted from HEK293 cells expressing FLAG-CD109 (293/FLAG-CD109) was immunoprecipitated with anti-FLAG affinity gel, and the co-precipitated proteins were analyzed by mass spectrometry and western blotting. Exosomal proteins were associated with CD109. We revealed the presence of CD109 in exosome fractions from conditioned medium of 293/FLAG-CD109. Moreover, the localization of CD109 in the exosome was demonstrated using immuno-electron microscopy. When we used HEK293 cells expressing FLAG-tagged truncated CD109, which does not contain the C-terminal region, the association of truncated CD109 with exosomes was not detected in conditioned medium. These findings indicate that CD109 is an exosomal protein and that the C-terminal region of CD109 is required for its presence in the exosome.

  17. "The Role ofL-arginine in Control of Apoptosis in Preimplantation Mouse Embryos Cultured in High Glucose Media "

    Directory of Open Access Journals (Sweden)

    Mohammad Barbarestani

    2004-06-01

    Full Text Available Maternal hyperglycemia causes delay in early stages of embryonic growth and development, higher incidence of congenital malformations and spontaneous miscarriage compared with those of non-diabetic conditions. High glucosis tratogenicity seems to be related to reduction of Nitric Oxide production (NO in hyperglycemic condition. In order to test this hypothesis, 2-cell stage embryos of normal mice were cultured with high concentration of glucose (30mM and different concentrations of L-arginine (5,10,20 mM or L-NAME, an NO syntase (NOS inhibitor. In the end of culture, blastocysts were stained by by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL technique and apoptotic cells were detected by using a Fluorescence microscope. Finally the amount of nitrite in the cultured media was assayed by Griess method. The results indicated that high glucose reduces Nitric Oxide production by preimplantation embryos and increases apoptosis of embryonic cells, but 5-20mM of L-arginine significantly increases Nitric Oxide production and decreases apoptosis. On the contrary L-NAME significantly inhibits the development of pre-implantation embryos. In conclusion, this study indicated that reduced nitric oxide production in high glucosis condition is a main factor for embryonic damage, and supplementation of high glucose media with L-arginine has an important role in prevention of high glucosis embryotoxicity

  18. Evaluation of three formulations of culture media for isolation of Brucella spp. regarding their ability to inhibit the growth of contaminating organisms.

    Science.gov (United States)

    Vicente, Acácia F; Antunes, João M A P; Lara, Gustavo H B; Mioni, Mateus S R; Allendorf, Susan D; Peres, Marina G; Appolinário, Camila M; Listoni, Fernando J P; Ribeiro, Marcio G; Megid, Jane

    2014-01-01

    Three culture media (Brucella agar, Farrell medium, and CITA) were compared for their effectiveness in inhibiting contamination and for isolating Brucella spp. One hundred lymph nodes from pigs (n = 50) and wild boars (n = 50) with lymphadenitis were collected in slaughterhouses in the State of São Paulo and were assessed on these three selective media for Brucella spp. All of the samples were negative for Brucella spp. on the three culture media. On the agar medium, fungal (70 plates) and Gram-positive bacterial (59 plates) contaminants were observed; in the CITA medium, the absence of fungal and Gram-positive bacteria on 15 plates was observed; no bacterial or fungal growth was observed on the Farrell media. The results demonstrated that the CITA and Farrell media inhibited the growth of contaminants better than the Brucella agar.

  19. Raman spectroscopy analysis of differences in composition of spent culture media of in vitro cultured preimplantation embryos isolated from normal and fat mice dams.

    Science.gov (United States)

    Fabian, Dušan; Kačmarová, Martina; Kubandová, Janka; Čikoš, Štefan; Koppel, Juraj

    2016-06-01

    The aim of the present study was to compare overall patterns of metabolic activity of in vitro cultured preimplantation embryos isolated from normal and fat mice dams by means of non-invasive profiling of spent culture media using Raman spectroscopy. To produce females with two different types of body condition (normal and fat), a previously established two-generation model was used, based on overfeeding of experimental mice during prenatal and early postnatal development. Embryos were isolated from spontaneously ovulating and naturally fertilized dams at the 2-cell stage of development and cultured to the blastocyst stage in synthetic oviductal medium KSOMaa. Embryos from fat mice (displaying significantly elevated body weight and fat) showed similar developmental capabilities in vitro as embryos isolated from normal control dams (displaying physiological body weight and fat). The results show that alterations in the composition of culture medium caused by the presence of developing mouse preimplantation embryos can be detected using Raman spectroscopy. Metabolic activity of embryos was reflected in evident changes in numerous band intensities in the 1620-1690cm(-1) (amide I) region and in the 1020-1140cm(-1) region of the Raman spectrum for KSOMaa. Moreover, multivariate analysis of spectral data proved that the composition of proteins and other organic compounds in spent samples obtained after the culture of embryos isolated from fat dams was different from that in spent samples obtained after the culture of embryos from control dams. This study demonstrates that metabolic activity of cultured preimplantation embryos might depend on the body condition of their donors.

  20. Culture and Art Channel network: media, culture and politics TV Cultura e arte: mídia, cultura e política

    Directory of Open Access Journals (Sweden)

    Claudio Bertolli Filho

    2007-05-01

    Full Text Available This paper focuses the short life of the Culture and Art Channel, a TV company created and supported by the Ministry of Culture in Brazil. The search for a perfect knowledge of how this public channel should be took it to the poor context of the cultural politics proposed by the administration of the former president Fernando Henrique Cardoso. The way the strategies of implementation and the answers to the critics were conducted also facilitated its failure. As a result, the television company did not have good indices of audience and its activities were closed down less than 2 years after its first broadcasting. Keywords: Culture and Arte channel network; public TV; cultural politics; Fernando Henrique Cardoso Administration; media and power. Este artigo tem como objetivo focar a curta trajetória de vida da TV Cultura e Arte, emissora criada e patrocinada pelo Ministério da Cultura do Brasil. A busca do entendimento do caminho tomada por esse canal público levou a situá-lo no contexto da precária política cultural adotada pela administração FHC e também das estratégias de legitimação da iniciativa e das respostas oferecidas aos críticos pelos idealizadores e responsáveis pelo funcionamento da TV Cultura e Arte. Resultado de tudo isso, a emissora contou com pouca audiência, encerrando suas atividades menos de dois anos depois de ter sido colocada no ar. Palavras-chave: TV Cultura e Arte. TV pública. Política cultural. Governo FHC. Mídia e poder.

  1. Selective enrichment media bias the types of Salmonella enterica strains isolated from mixed strain cultures and complex enrichment broths.

    Science.gov (United States)

    Gorski, Lisa

    2012-01-01

    For foodborne outbreak investigations it can be difficult to isolate the relevant strain from food and/or environmental sources. If the sample is contaminated by more than one strain of the pathogen the relevant strain might be missed. In this study mixed cultures of Salmonella enterica were grown in one set of standard enrichment media to see if culture bias patterns emerged. Nineteen strains representing four serogroups and ten serotypes were compared in four-strain mixtures in Salmonella-only and in cattle fecal culture enrichment backgrounds using Salmonella enrichment media. One or more strain(s) emerged as dominant in each mixture. No serotype was most fit, but strains of serogroups C2 and E were more likely to dominate enrichment culture mixtures than strains of serogroups B or C1. Different versions of Rappaport-Vassiliadis (RV) medium gave different patterns of strain dominance in both Salmonella-only and fecal enrichment culture backgrounds. The fittest strains belonged to serogroups C1, C2, and E, and included strains of S. Infantis, S. Thompson S. Newport, S. 6,8:d:-, and S. Give. Strains of serogroup B, which included serotypes often seen in outbreaks such as S. Typhimurium, S. Saintpaul, and S. Schwarzengrund were less likely to emerge as dominant strains in the mixtures when using standard RV as part of the enrichment. Using a more nutrient-rich version of RV as part of the protocol led to a different pattern of strains emerging, however some were still present in very low numbers in the resulting population. These results indicate that outbreak investigations of food and/or other environmental samples should include multiple enrichment protocols to ensure isolation of target strains of Salmonella.

  2. A randomized control comparison study of culture media (HTF versus P1) for human in vitro fertilization.

    Science.gov (United States)

    Artini, Paolo G; Valentino, Valeria; Cela, Vito; Cristello, Francesca; Vitè, Alessandra; Genazzani, Andrea R

    2004-10-15

    It is now widely accepted that increasing the number of replacement embryos (>3 embryos per embryo transfer [ET]) is associated with an increased risk of multiple pregnancies. While embryo reduction is often proposed when there is a high risk of multiple pregnancies, it is a difficult decision for the couple. For this reason, different studies have focused on single embryo transfer, more precisely blastocyst transfer. The aim of the study is to confirm that phosphate-free culture media can be used to generate greater quality embryos. We carried out a study to compare the efficacy of human tubal fluid (HTF) versus preimplantation stage one (P1) as culture media for assisted reproductive therapy (ART). In 109 nonselected patients, we obtained an embryo fertilization rate with HTF and P1 culture media of 58.6 and 62.5% (P = 0.003), respectively. After 48 and 72 h, the morphology was similar for both P1 and HTF embryos in most patients. However, in the same patients, when HTF embryo quality was low (15.4%), P1 embryo quality was significantly higher 68.7% (P = 0.002). Some embryos were transferred at 48 h and some at 72 h after retrieval, in a randomized manner. We transferred a maximum of up to three embryos per ET. The implantation rate was significantly different; at 48 h, it was 6.8 and 12.2% for HTF and P1, respectively (P = 0.02). The pregnancy rate was 17.1% for HTF embryos and 23.7% for P1 embryos (P = 0.02). Therefore, we observed a significant difference between P1 and HTF in the fertilization rate, in embryo quality, in implantation rate and in pregnancy rate. But the most important difference between this study and others is that every patient was the control of herself, so we eliminated every variable.

  3. Effects of different media on vegetative growth of two Lilium cultivars in soilless culture

    Directory of Open Access Journals (Sweden)

    A. Khaligy

    2011-07-01

    Full Text Available Strong, tall and healthy stems, as well as green background by means of leaves, are very important characters in marketing of cut flowers including Lily (Lilium. Also, in order to reuse the bulbs for next crop production, they should have enough stored materials and perimeter. This investigation was conducted to evaluate the effects of different media on some vegetative characters of two Lillium cultivars in greenhouse conditions and determine the most suitable medium. The experiment was based on randomized complete design with two cultivars namely Bernini (Oriental and Cebdazzle (Asiatic and 4 replicates. Ten treatments of the experiment were organic and mineral media including cocopeat, sand, vermiculite, perlite and mixture of each two media at equal volumes (50: 50. All the pots were fertigated daily by 250 ml of Hoagland half strength solution. Means were compared by Duncan multiple range test at 5% probability level. The results showed superiority of cocopeat over other media in terms of plant chlorophyll content, fresh and dry weight of leaves and stem, leaf area, height, stem diameter, number of leaves, and bulb perimeter in both cultivars. Root length of both cultivars reached the highest using the mixture of perlite and cocopeat. The performance of Cebdazzle cv. against Bernini cv. regarding fresh and dry weight of leaves, leaf area, stem diameter, number of leaves, stem dry weight and root length were better in all the media. While, mean chlorophyll content and fresh weight of stems in Bernini was significantly higher than Cebdazzle.

  4. 传媒转型时期管理文化软实力构建%Construction of Management Culture Soft Power in Media Transition Period

    Institute of Scientific and Technical Information of China (English)

    文崇坚; 帅锦平

    2012-01-01

    向管理要效益是市场经济的使然.管理的本质就是一种文化现象.管理文化是一种强大的软实力.只有构建和谐的管理文化的柜架,做到“管”与“理”的有机搭配,管理才会产生它本质上的意义,推进企业健康发展.西方文明是中国传媒文化需要吸收的精华所在,源远流长的中华文化给传媒文化以厚实的根基.传媒管理文化的形成具有自己的模式,构建卓越的传媒文化需要全方位探索.企业文化实质就是企业家的文化,传媒领袖人物个人魅力至关重要.强化传媒企业的社会责任,是其外在形象之所在.塑造传媒人为新闻事业的献身精神,是其内在依托.注重跨国文化沟通,是全球经济一体化的必然选择.打造传媒品牌,是传播管理文化的灵魂.文化是柄双刃剑.中国传媒业应当大力发挥传播管理文化的积极功能,克服其消极功能.只有这样,才能彰显传媒管理文化绚丽的光华.传媒管理文化软实力如何构建,当下传媒学界颇有研究的必要.%Media-cultural management and cultural studies is to enhance the country's cultural soft power is an important topic. Media is a product of culture, the essence of management is a cultural phenomenon. Western civilization is the Chinese media need to absorb the essence of culture, the heritage of Chinese culture to media culture with thick roots. The formation of the media management culture has its own pattern, and cultural needs of the media to build outstanding all-round exploration. Essence of corporate culture and cultural entrepreneurs, media leaders charisma is essential. Strengthen the social responsibility of media companies, is the external image of lies. News media to shape human dedication to the cause, is its inherent support. Focus on cross-cultural communication, global economic integration is an inevitable choice. Create a media brand, is to spread the management culture of the soul. Culture is

  5. Evaluation of culture media for the production of secondary metabolites in a natural products screening program.

    Science.gov (United States)

    Vandermolen, Karen M; Raja, Huzefa A; El-Elimat, Tamam; Oberlies, Nicholas H

    2013-12-17

    Variation in the growing environment can have significant impacts on the quantity and diversity of fungal secondary metabolites. In the industrial setting, optimization of growing conditions can lead to significantly increased production of a compound of interest. Such optimization becomes challenging in a drug-discovery screening situation, as the ideal conditions for one organism may induce poor metabolic diversity for a different organism. Here, the impact of different media types, including six liquid media and five solid media, on the secondary metabolite production of three fungal strains was examined in the context of the drug-discovery screening process. The relative production of marker compounds was used to evaluate the usefulness and reliability of each medium for the purpose of producing secondary metabolites.

  6. 媒介文化视域下的意识形态传播策略探析%Ideology Communication Strategies in a Media Cultural Perspective

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    媒介文化在意识形态传播中发挥着重要的载体功能。然而媒介文化是表达多元话语的场域,不尽是服务于意识形态,它们之间存在着既合作又疏离的关系。因此,意识形态需要运用一定的传播策略争取媒介文化的话语主导权。当下,我国主流意识形态传播需要融进媒介文化中,积极回应媒介文化所表达的多元话语,并适度对媒介文化进行信息控制,实现我国主流意识形态能够引导媒介文化中的多元话语表达。%Media culture communication plays an important carrier of the ideological function. However media cul-ture is expression of pluralistic discourse filed,it is not entirely in the service of ideology,between them is both cooperation and alienation. Therefore,need to use communication strategies for media culture ideology discourse dominance. At present,our country mainstream ideology spread into media culture,Actively respond to the media culture express multiple words,and appropriate to control the information media culture,realize the mainstream ideology discourse dominance in the media culture.

  7. A qualitative study exploring visible components of organizational culture: what influences the use of psychotropic medicines in nursing homes?

    Science.gov (United States)

    Sawan, Mouna J; Jeon, Yun-Hee; Fois, Romano J; Chen, Timothy F

    2016-10-01

    The influence of organizational culture on how psychotropic medicines are used in nursing homes has not been extensively studied. Schein's theory provides a framework for examining organizational culture which begins with the exploration of visible components of an organization such as behaviors, structures, and processes. This study aimed to identify key visible components related to the use of psychotropic medicines in nursing homes. A qualitative study was conducted in eight nursing homes in Sydney, Australia. Purposive sampling was used to conduct semi-structured interviews with 40 participants representing a broad range of health disciplines. Thematic analysis was used to derive concepts. Three visible components were related to psychotropic medicine use. These were drugs and therapeutics committee meetings, pharmacist led medication management reviews and formal and informal meetings with residents and their families. We found that only a few nursing homes utilized drugs and therapeutics committee meetings to address the overuse of psychotropic medicines. Pharmacist led medication management reviews provided a lever to minimize inappropriate psychotropic prescribing for a number of nursing homes; however, in others it was used as a box-ticking exercise. We also found that some nursing homes used meetings with residents and their families to review the use of psychotropic medicines. This study was the first to illustrate that visible components of organizational culture do influence the use of psychotropic medicines and explains in detail what of the culture needs to be addressed to reduce inappropriate psychotropic prescribing.

  8. People’s confidence in innovation: A component of the entrepreneurial mindset, embedded in gender and culture

    DEFF Research Database (Denmark)

    Ashourizadeh, Shayegheh; Hassannezhad Chavousi, Zohreh; Schøtt, Thomas

    2014-01-01

    especially high for entrepreneurs and by being related to other components such as self-efficacy, opportunity-alertness, risk-propensity and role-modelling. People’s confidence are unrelated to their gender, unexpectedly, but are especially high in traditional culture, as hypothesised. Confidence...

  9. COMPONENTS ANALYSIS OF ORGANIZATIONAL CULTURE ON WORK AND JOB SATISFACTION IN A PUBLIC INSTITUTION

    Directory of Open Access Journals (Sweden)

    ILEANA DANUȚ

    2016-02-01

    Full Text Available Public sector organizations face increasing pressure to adapt to new requirements from users, generated by phenomena such as the expansion of information technologies, globalization, increasing citizens' awareness of their rights. These new requirements involve changes in cultures of such organizations. While the rules, procedures, activities of public organizations are constantly evolving, culture of an organization change is an effort that requires a very long period. To some extent, cultural change process is lengthy due to the resistance that may arise in the event of planned changes in the culture of an organization. To facilitate the process of cultural change is necessary to know the particularities of organizational culture, in particular those related to work, job satisfaction and human resources policies. In this work we intend to research and interpret employee perceptions of organizational culture Gendarmerie Calarasi on issues related to work, job satisfaction and human resources policies to improve those aspects aiming human resource satisfaction.

  10. Microorganisms detection in culture media by neutron radiography technique; Deteccao de microorganismos em meios de cultura pela tecnica de neutrongrafia

    Energy Technology Data Exchange (ETDEWEB)

    Wacha, Reinaldo; Lopes, Joana D' Arc L.; Crispim, Verginia R. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia. Programa de Engenharia Nuclear; Lage, Claudia [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Biologia Carlos Chagas Filho

    1998-12-01

    The study aims to obtain a more effective and faster method forthe detection of bacteria in several culture media, such as portable water and blood. After the process of growth in the culture medium, separation and suspension in buffer solution based on boron, the bacteria are deposited in track detectors that are submitted to thermal neutron beams (neutron flux: 2,2 x 10{sup 5} n.cm{sup -2}.s{sup -1}), resulting from the channel J-9 of the Argonauta research reactor, from the Nuclear Engineering B(n,{alpha}) Li and, after having been revealed, are analyzed by an optical microscope that allows to the identification of the tracks of the alpha particles. (author)

  11. Demography of zooplankton (Anuraeopsis fissa, Brachionus rubens and Moina macrocopa) fed Chlorella vulgaris and Scenedesmus acutus cultured on different media.

    Science.gov (United States)

    Morales-Ventura, Jesús; Nandini, S; Sarma, S S S; Castellanos-Páez, Maria Elena

    2012-09-01

    Generally zooplankton growth is often limited by the quality of their algal diet. A cheaper common practice in aquaculture, is to culture algae with fertilizers; however, the demography of zooplankton when fed these algae has not yet been evaluated. We studied the population growth and life table demography of the rotifers Anuraeopsis fissa and Brachionus rubens, and the cladoceran Moina macrocopa. For this, the algae Scenedesmus acutus or Chlorella vulgaris were cultured on defined (Bold's basal) medium or the commercial liquid fertilizer (Bayfolan). Experiments were conducted at one algal concentration 1.0 x 10(6) cells/mL of C. vulgaris or its equivalent dry weight of 0.5 x 10(6) cells/mL of S. acutus. The population dynamics were tested at 23 +/- 1 degrees C in 100 mL transparent jars, each with 50mL of the test medium, with an initial density of 0.5indiv/mL, for a total of 48 test jars (3 zooplankton 2 algal species x 2 culture media x 4 replicates). For the life table experiments with M. macrocopa, we introduced 10 neonates (liquid fertilizer as compared to the other diets. The growth rates of M. macrocopa ranged from 0.1 to 0.38/d, and were highest with diets of C. vulgaris cultured in Bold medium and S. acutus cultured in fertilizer. Thus, regardless of the culture medium used, the growth rates of the evaluated zooplankton species were higher with Chlorella than with Scenedesmus. The peak population density was highest (2 800ind/mL) for A. fissa fed Chlorella that was cultured on liquid fertilizers, while B. rubens and M. macrocopa had peak abundances of 480 and 12ind/mL, respectively under similar conditions.

  12. CD109 is a component of exosome secreted from cultured cells

    Energy Technology Data Exchange (ETDEWEB)

    Sakakura, Hiroki [Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya (Japan); Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya (Japan); Mii, Shinji [Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya (Japan); Hagiwara, Sumitaka [Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya (Japan); Department of Head and Neck Surgery, Aichi Cancer Center Hospital, Nagoya (Japan); Kato, Takuya [Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya (Japan); Yamamoto, Noriyuki; Hibi, Hideharu [Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya (Japan); Takahashi, Masahide, E-mail: mtakaha@med.nagoya-u.ac.jp [Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya (Japan); Murakumo, Yoshiki, E-mail: murakumo@med.kitasato-u.ac.jp [Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya (Japan); Department of Pathology, Kitasato University School of Medicine, Sagamihara, Kanagawa (Japan)

    2016-01-22

    Exosomes are 50–100-nm-diameter membrane vesicles released from various types of cells. Exosomes retain proteins, mRNAs and miRNAs, which can be transported to surrounding cells. CD109 is a glycosylphosphatidylinositol-anchored glycoprotein, and is released from the cell surface to the culture medium in vitro. Recently, it was reported that secreted CD109 from the cell surface downregulates transforming growth factor-β signaling in human keratinocytes. In this study, we revealed that CD109 is a component of the exosome in conditioned medium. FLAG-tagged human CD109 (FLAG-CD109) in conditioned medium secreted from HEK293 cells expressing FLAG-CD109 (293/FLAG-CD109) was immunoprecipitated with anti-FLAG affinity gel, and the co-precipitated proteins were analyzed by mass spectrometry and western blotting. Exosomal proteins were associated with CD109. We revealed the presence of CD109 in exosome fractions from conditioned medium of 293/FLAG-CD109. Moreover, the localization of CD109 in the exosome was demonstrated using immuno-electron microscopy. When we used HEK293 cells expressing FLAG-tagged truncated CD109, which does not contain the C-terminal region, the association of truncated CD109 with exosomes was not detected in conditioned medium. These findings indicate that CD109 is an exosomal protein and that the C-terminal region of CD109 is required for its presence in the exosome. - Highlights: • CD109 is an exosomal protein. • The C-terminal region of CD109 is required for its presence in the exosome. • Part of the secreted CD109 is present in the exosome-free fraction in the conditioned medium.

  13. Battling for Shared Culture between Indonesia and Malaysia In The Social Media Era

    OpenAIRE

    Liliek Adelina Suhardjono

    2012-01-01

    This is a research paper that takes as its central issue recent contestations and negotiations between Indonesia and Malaysia regarding certain forms of these countries’ intangible cultural heritage. In recent years, there has been an increasing incident in relation with cultural conflicts that happens between these two neighboring countries. These cultural conflicts specifically referred to the traditional performance arts, which has been hereditarily bequeathed by their respective ancestors...

  14. The Impact of Social Media and Crowdsourcing on Organizational Innovation Culture

    DEFF Research Database (Denmark)

    Scupola, Ada; Nicolajsen, Hanne Westh

    innovation culture using theory on organizational culture and crowdsourcing. The analysis shows that the organizational crowdsourcing event has supported an innovation culture change in the case company towards a more open approach to innovation; creating a new and different awareness of innovation, allowing...... for internal process innovations, empowering the employees, supporting knowledge work and collaboration across the organization to a new extent and overcoming the traditional hierarchy in the organization....

  15. Innovative Approaches Using Lichen Enriched Media to Improve Isolation and Culturability of Lichen Associated Bacteria

    OpenAIRE

    Biosca, Elena G.; Flores, Raquel; Ricardo D Santander; D?ez-Gil, Jos? Luis; Barreno, Eva

    2016-01-01

    Lichens, self-supporting mutualistic associations between a fungal partner and one or more photosynthetic partners, also harbor non-photosynthetic bacteria. The diversity and contribution of these bacteria to the functioning of lichen symbiosis have recently begun to be studied, often by culture-independent techniques due to difficulties in their isolation and culture. However, culturing as yet unculturable lichenic bacteria is critical to unravel their potential functional roles in lichen sy...

  16. Battling for Shared Culture between Indonesia and Malaysia In The Social Media Era

    National Research Council Canada - National Science Library

    Liliek Adelina

    2012-01-01

    This is a research paper that takes as its central issue recent contestations and negotiations between Indonesia and Malaysia regarding certain forms of these countries intangible cultural heritage...

  17. 媒介研究中的科技与文化--对媒介决定论的再思考%Technology and Culture in the Study of Media:Rethinking of Media Determinism

    Institute of Scientific and Technical Information of China (English)

    罗崇宏

    2014-01-01

    There are two quite different views in the study of media culture:media determinism,and the theory of social and cultural choice.Media determinism says that new technology can bring about new forms of social culture .But social and cultural determinism is thought to have social and cultural context of potential“call”.And then a corresponding form of science and technology arise.With the emergence of new media,media determinism seems to occupy the mainstream place.But the theory of choice can also play a good role .There should be a complementary relationship between them . The emergence of new media is supposed to the“resultant force”of the progress of technology and culture of the“call”.%在媒介文化研究中存在着两种截然不同的观点:媒介决定论和社会文化选择论。媒介决定论认为新科技带来新的社会文化形式;而社会文化选择论则认为先有社会文化语境的潜在“召唤”,然后才出现相应的科技形式。随着新媒介的出现,媒介决定论似乎占据主流,但是文化选择论对媒介决定论又起到很好的补充作用,它们之间是一种互补关系。新媒介的出现应该是在科技的进步和文化“召唤”的合力作用下产生的。

  18. Determination of amino acids in cell culture and fermentation broth media using anion-exchange chromatography with integrated pulsed amperometric detection.

    Science.gov (United States)

    Hanko, Valoran P; Rohrer, Jeffrey S

    2004-01-01

    Cell culture and fermentation broth media are used in the manufacture of biotherapeutics and many other biological materials. Characterizing the amino acid composition in cell culture and fermentation broth media is important because deficiencies in these nutrients can reduce desired yields or alter final product quality. Anion-exchange (AE) chromatography using sodium hydroxide (NaOH) and sodium acetate gradients, coupled with integrated pulsed amperometric detection (IPAD), determines amino acids without sample derivatization. AE-IPAD also detects carbohydrates, glycols, and sugar alcohols. The presence of these compounds, often at high concentrations in cell culture and fermentation broth media, can complicate amino acid determinations. To determine whether these samples can be analyzed without sample preparation, we studied the effects of altering and extending the initial NaOH eluent concentration on the retention of 42 different carbohydrates and related compounds, 30 amino acids and related compounds, and 3 additional compounds. We found that carbohydrate retention is impacted in a manner different from that of amino acid retention by a change in [NaOH]. We used this selectivity difference to design amino acid determinations of diluted cell culture and fermentation broth media, including Bacto yeast extract-peptone-dextrose (yeast culture medium) broth, Luria-Bertani (bacterial culture medium) broth, and minimal essential medium and serum-free protein-free hybridoma medium (mammalian cell culture media). These media were selected as representatives for both prokaryotic and eukaryotic culture systems capable of challenging the analytical technique presented in this paper. Glucose up to 10mM (0.2%, w/w) did not interfere with the chromatography, or decrease recovery greater than 20%, for the common amino acids arginine, lysine, alanine, threonine, glycine, valine, serine, proline, isoleucine, leucine, methionine, histidine, phenylalanine, glutamate, aspartate

  19. European MEDIA Programme: the role of 'language' and 'visual images' in the processes of constructing European culture and identity.

    Science.gov (United States)

    Bozić-Vrbancić, Senka; Vrbancić, Mario; Orlić, Olga

    2008-12-01

    Questions of diversity and multiculturalism are at the heart of many discussions on European supranational identity within contemporary anthropology, sociology, cultural studies, linguistics and so on. Since we are living in a period marked by the economic and political changes which emerged after European unification, a call for a new analysis of heterogeneity, cultural difference and issues of belonging is not surprising. This call has been fuelled by the European Union's concern with "culture" as one of the main driving forces for constructing "European identity". While the official European policy describes European culture as common to all Europeans, Europe is also-seen as representing "unity in diversity". By analysing contemporary European MEDIA policies and programs this article attempts to contribute to a small but growing body of work that explores what role "language" and "visual images" play in the process of constructing European culture and supranational European identity. More specifically, the article explores the complex articulation of language and culture in order to analyse supranational imaginary of European identity as it is expressed through the simple slogan "Europe: unity in diversity". We initially grounded our interest in the politics of identity within the European Union within theoretical frameworks of "power and knowledge" and "identity and subjectivity". We consider contemporary debates in social sciences and humanities over the concepts of language", "culture" and "identity" as inseparable from each other (Ahmed 2000; Brah 1996, 2000; Butler 1993, Derrida 1981; Gilroy 2004; Laclau 1990). Cultural and postcolonial studies theorists (e.g. Brah 1996; Bhabha 1994; Hall 1992, 1996, among others) argue that concepts of "culture" and "identity" signify a historically variable nexus of social meanings. That is to say, "culture" and "identity" are discursive articulations. According to this view, "culture" and "identity" are not separate

  20. Plasma-on-chip device for stable irradiation of cells cultured in media with a low-temperature atmospheric pressure plasma.

    Science.gov (United States)

    Okada, Tomohiro; Chang, Chun-Yao; Kobayashi, Mime; Shimizu, Tetsuji; Sasaki, Minoru; Kumagai, Shinya

    2016-09-01

    We have developed a micro electromechanical systems (MEMS) device which enables plasma treatment for cells cultured in media. The device, referred to as the plasma-on-chip, comprises microwells and microplasma sources fabricated together in a single chip. The microwells have through-holes between the microwells and microplasma sources. Each microplasma source is located on the backside of each microwells. The reactive components generated by the microplasma sources pass through the through-holes and reach cells cultured in the microwells. In this study, a plasma-on-chip device was modified for a stable plasma treatment. The use of a dielectric barrier discharge (DBD) technique allowed a stable plasma treatment up to 3 min. The plasma-on-chip with the original electrode configuration typically had the maximum stable operation time of around 1 min. Spectral analysis of the plasma identified reactive species such as O and OH radicals that can affect the activity of cells. Plasma treatment was successfully performed on yeast (Saccharomyces cerevisiae) and green algae (Chlorella) cells. While no apparent change was observed with yeast, the treatment degraded the activity of the Chlorella cells and decreased their fluorescence. The device has the potential to help understand interactions between plasma and cells.

  1. Eyewitnesses of History: Italian Amateur Cinema as Cultural Heritage and Source for Audiovisual and Media Production

    NARCIS (Netherlands)

    Simoni, Paolo

    2015-01-01

    abstractThe role of amateur cinema as archival material in Italian media productions has only recently been discovered. Italy, as opposed to other European countries, lacked a local, regional and national policy for the collection and preservation of private audiovisual documents, which led, as a re

  2. Communicating Culture in the 21st Century: The Power of Media-Enhanced Immersive Storytelling

    Science.gov (United States)

    Stogner, Maggie Burnette

    2011-01-01

    This paper explores the potential of digital media technologies and new storytelling techniques in giving objects an emotional dimension and thus encouraging affective learning. The use of new immersive and participatory techniques is a means of contextualizing real objects, and perhaps a more effective way to reach diverse audiences and create…

  3. "On Behalf of a Shared World": Arendtian Politics in a Culture of Youth Media Participation

    Science.gov (United States)

    Poyntz, Stuart R.

    2009-01-01

    More than 30 years since Hannah Arendt's death in 1975 at the age of 69, her novel theory of the public realm continues to attract attention and debate. In this article, the author contributes to this discussion by drawing on Arendt's theory of public life to investigate the space of youth media production in relation to questions of democratic…

  4. Mexico: The Role of the Participatory Media in Immigration/Emigration as Culture and Political Economy.

    Science.gov (United States)

    Oseguera, A. Anthony

    Mexican mass media, especially television, incorporates an abundance of American programming and serves as a catalyst to motivate lower strata Mexicans to pursue life in the United States, resulting in a tremendous influx of both legal and illegal Mexicans and other Latin Americans to the United States. Although Mexico benefits because many…

  5. Of Sound Mind: Mental Distress and Sound in Twentieth-Century Media Culture

    NARCIS (Netherlands)

    Birdsall, Carolyn; Siewert, Senta

    2013-01-01

    abstractThis article seeks to specify the representation of mental disturbance in sound media during the twentieth century. It engages perspectives on societal and technological change across the twentieth century as crucial for aesthetic strategies developed in radio and sound film production. The

  6. Of Sound Mind: Mental Distress and Sound in Twentieth-Century Media Culture

    NARCIS (Netherlands)

    Birdsall, C.; Siewert, S.

    2013-01-01

    This article seeks to specify the representation of mental disturbance in sound media during the twentieth century. It engages perspectives on societal and technological change across the twentieth century as crucial for aesthetic strategies developed in radio and sound film production. The analysis

  7. Italianization Accomplished. Forms and Structures of Albanian Television’s Dependency on Italian Media and Culture

    NARCIS (Netherlands)

    Carelli, Paolo

    2014-01-01

    abstractAfter the fall of the Berlin Wall and the end of communist regimes in 1989, national media systems of the Eastern European countries belonging to the Soviet bloc began a process of transformation along the way of liberalization and commercialization. In Albania, this process occurred in diff

  8. MODERN MEDIA EDUCATION MODELS

    Directory of Open Access Journals (Sweden)

    Alexander Fedorov

    2011-03-01

    Full Text Available The author supposed that media education models can be divided into the following groups:- educational-information models (the study of the theory, history, language of media culture, etc., based on the cultural, aesthetic, semiotic, socio-cultural theories of media education;- educational-ethical models (the study of moral, religions, philosophical problems relying on the ethic, religious, ideological, ecological, protectionist theories of media education;- pragmatic models (practical media technology training, based on the uses and gratifications and ‘practical’ theories of media education;- aesthetical models (aimed above all at the development of the artistic taste and enriching the skills of analysis of the best media culture examples. Relies on the aesthetical (art and cultural studies theory; - socio-cultural models (socio-cultural development of a creative personality as to the perception, imagination, visual memory, interpretation analysis, autonomic critical thinking, relying on the cultural studies, semiotic, ethic models of media education.

  9. Impact of social media as an instructional component on content knowledge, attitudes, and public engagement related to global climate change

    Science.gov (United States)

    Greenberg, Sallie E.

    Social media (SM) are considered important avenues to reach citizens and engage them in social change. Given the widespread use of SM and their potential to enhance communication, they could also have significant influence when used as an educational tool. Educators are exploring whether classroom SM use has instructional benefits, such as enhancing interactivity and engagement. It is critical to understand the potential of SM for creating meaningful learning environments and public engagement pathways. Much work remains to understand the use of SM in this context and how to use them effectively. This study draws on active learning theory to examine the impact of SM as an instructional component with community college students learning to make connections among science, social responsibility, and global understanding in an environmental biology course (the Course). Using global climate change as a theme, the Course included a Facebook instructional component. A pretest--posttest, nonrandomized comparison group design was used to measure the impact of Facebook as an integrated component of the Course. The treatment and comparison groups were determined to be comparable based on demographics, access and ownership of digital devices, and SM use despite non-random assignment. No statistically significant differences were found between groups on these factors. The intervention consisted of semester-long required use of Facebook for the treatment group. The impact of the SM intervention was measured in three areas: (a) content knowledge, (b) attitudes toward climate change, and (c) public engagement actions and intentions to act. At the conclusion of the Course, no discernable difference was measured in content knowledge gains between the two groups. However, students who used Facebook experienced statistically significant differences in attitude, becoming increasingly concerned about global climate change. The comparison group demonstrated statistically significant

  10. A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

    OpenAIRE

    Ascensión Martínez-Márquez; Jaime Morante-Carriel; Karla Ramírez-Estrada; Rosa Cusido; Susana Sellés-Marchart; Javier Palazon; Maria Angeles Pedreño; Roque Bru-Martínez

    2015-01-01

    One of the major intent of metabolic engineering in cell culture systems is to increase yields of secondary metabolites. Efficient transformation methods are a priority to successfully apply metabolic engineering to cell cultures of plants that produce bioactive or therapeutic compounds, such as Vitis vinifera and Taxus x media. The aim of this study was to establish a reliable method to transform non-embryogenic cell cultures of these species. The V. vinifera cv. Gamay/cv. Monastrell cell li...

  11. The regional component of university courses of “Russian language and culture of speech” at the national branch

    Directory of Open Access Journals (Sweden)

    Makhmutova A. S.

    2015-01-01

    Full Text Available The article is devoted to the formation of linguistic, communicative and cultural competence among students bilinguals in teaching Russian language and speech culture. The authors put forward the thesis that the training of specialists in the conditions of bilingualism re quires not only a higher level of learning a second language, but also a qualitatively different level of comprehension. It is proved that the discipline “Russian and the culture of speech” assumes formation of communicative and culturological competence of the higher education institutions, which are trained on two-profile offices taking into account a regional component. Relevance of research in this area is increasing each year since the treatment of the person as the center of all systems of cognition highlights in the pedagogical University the formation of the modern language personality, capable of effective professional activity. The functional approach provides detection of features of functioning of language units in different spheres of public work. The communicative principle means that acquisition of Russian as a means of communication happens in the course of speech practice most approached to process of communication in Russian. The detection of similarity and distinctions between languages, interlingual comparison promotes establishment and a lexical interference. The linguistic-cultural principle provides understanding of language as the phenomena of culture and is connected with ability to define national-cultural components of linguistic units - words, phraseological combinations, grammatical categories of the text. Involvement of regional component as a basis in practice of teaching Russian and culture of speech increases efficiency of all process of training of students of national branch.

  12. Influence of Culture Media on Biofilm Formation by Candida Species and Response of Sessile Cells to Antifungals and Oxidative Stress

    Science.gov (United States)

    Serrano-Fujarte, Isela; Reyna-López, Georgina Elena; Martínez-Gámez, Ma. Alejandrina; Vega-González, Arturo; Cuéllar-Cruz, Mayra

    2015-01-01

    The aims of the study were to evaluate the influence of culture media on biofilm formation by C. albicans, C. glabrata, C. krusei, and C. parapsilosis and to investigate the responses of sessile cells to antifungals and reactive oxygen species (ROS) as compared to planktonic cells. For biofilm formation, the Candida species were grown at different periods of time in YP or YNB media supplemented or not with 0.2 or 2% glucose. Sessile and planktonic cells were exposed to increasing concentrations of antifungals, H2O2, menadione or silver nanoparticles (AgNPs). Biofilms were observed by scanning electron microscopy (SEM) and quantified by the XTT assay. C. albicans formed biofilms preferentially in YPD containing 2% glucose (YPD/2%), C. glabrata in glucose-free YNB or supplemented with 0.2% glucose (YNB/0.2%), while C. krusei and C. parapsilosis preferred YP, YPD/0.2%, and YPD/2%. Interestingly, only C. albicans produced an exopolymeric matrix. This is the first report dealing with the in vitro effect of the culture medium and glucose on the formation of biofilms in four Candida species as well as the resistance of sessile cells to antifungals, AgNPs, and ROS. Our results suggest that candidiasis in vivo is a multifactorial and complex process where the nutritional conditions, the human immune system, and the adaptability of the pathogen should be considered altogether to provide an effective treatment of the patient. PMID:25705688

  13. Tswanarising global gayness: the 'unAfrican' argument, Western gay media imagery, local responses and gay culture in Botswana.

    Science.gov (United States)

    McAllister, John

    2013-01-01

    This paper is a strategic intervention in the debate over the value of globalised gay identity for emerging sexual minority communities in the South. Focusing on self-identifying gay men in Botswana using semi-structured interviews, it explores their views of what characterises 'modern gay culture' and relates these to international media clichés of a glamorous, stylish, hedonistic gayness. I argue that identifying with what is so visibly a Western image of gayness exposes sexual minority communities to the most dangerous of the justifications for homophobia in Africa, the argument that sexual dissidence is a neo-colonial conspiracy to subvert 'African values'. The 'unAfrican' argument has to be taken very seriously, not only because it taps into the intense, conflicted emotions at the heart of the post-colonial condition, but also because it contains an undeniable germ of truth. This poses a dilemma, since global gay discourses, including the media clichés, are an important source of inspiration for African sexual minorities. A communication activism strategy is proposed to undermine the unAfrican argument by cultivating and asserting the 'tswanarisation' of gay culture in Botswana that is already taking place. A similar strategy may also be effective in other African societies.

  14. Influence of culture media on biofilm formation by Candida species and response of sessile cells to antifungals and oxidative stress.

    Science.gov (United States)

    Serrano-Fujarte, Isela; López-Romero, Everardo; Reyna-López, Georgina Elena; Martínez-Gámez, Ma Alejandrina; Vega-González, Arturo; Cuéllar-Cruz, Mayra

    2015-01-01

    The aims of the study were to evaluate the influence of culture media on biofilm formation by C. albicans, C. glabrata, C. krusei, and C. parapsilosis and to investigate the responses of sessile cells to antifungals and reactive oxygen species (ROS) as compared to planktonic cells. For biofilm formation, the Candida species were grown at different periods of time in YP or YNB media supplemented or not with 0.2 or 2% glucose. Sessile and planktonic cells were exposed to increasing concentrations of antifungals, H2O2, menadione or silver nanoparticles (AgNPs). Biofilms were observed by scanning electron microscopy (SEM) and quantified by the XTT assay. C. albicans formed biofilms preferentially in YPD containing 2% glucose (YPD/2%), C. glabrata in glucose-free YNB or supplemented with 0.2% glucose (YNB/0.2%), while C. krusei and C. parapsilosis preferred YP, YPD/0.2%, and YPD/2%. Interestingly, only C. albicans produced an exopolymeric matrix. This is the first report dealing with the in vitro effect of the culture medium and glucose on the formation of biofilms in four Candida species as well as the resistance of sessile cells to antifungals, AgNPs, and ROS. Our results suggest that candidiasis in vivo is a multifactorial and complex process where the nutritional conditions, the human immune system, and the adaptability of the pathogen should be considered altogether to provide an effective treatment of the patient.

  15. Lupin peptone as a replacement for animal-derived peptone in rich culture media for yeast.

    Science.gov (United States)

    Chapman, Melissa; Mariano, Krichelle; Macreadie, Ian

    2015-02-01

    Lupin peptone was shown to be a suitable replacement for traditional bacteriological peptone in the culture of Candida glabrata, Candida albicans and Saccharomyces cerevisiae. This new medium formulation allows yeast researchers to increase safety and to eliminate the use of animal products for the culture of yeast in rich medium. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. A Double-Edged Sword: Social Media as a Tool of Online Disinhibition Regarding American Sign Language and Deaf Cultural Experience Marginalization, and as a Tool of Cultural and Linguistic Exposure

    Directory of Open Access Journals (Sweden)

    K. Crom Saunders

    2016-01-01

    Full Text Available Social media has become a venue for social awareness and change through forum discussions and exchange of viewpoints and information. The rate at which awareness and cultural understanding regarding specific issues has not been quantified, but examining awareness about issues relevant to American Sign Language (ASL and American Deaf culture indicates that progress in increasing awareness and cultural understanding via social media faces greater friction and less progress compared to issues relevant to other causes and communities, such as feminism, the lesbian, gay, bisexual, and transgender (LGBT community, or people of color. The research included in this article examines online disinhibition, cyberbullying, and audism as it appears in the real world and online, advocacy for and against Deafness as a cultural identity, and a history of how Deaf people are represented in different forms of media, including social media. The research itself is also examined in terms of who conducts the research. The few incidents of social media serving the Deaf community in a more positive manner are also examined. This is to provide contrast to determine which factors may contribute to greater progress in fostering greater awareness of Deaf cultural issues without the seemingly constant presence of resistance and lack of empathy for the Deaf community’s perspectives on ASL and Deaf culture.

  17. Correlation between mastitis occurrence and the count of microorganisms in bulk raw milk of bovine dairy herds in four selective culture media.

    Science.gov (United States)

    Souto, Luís I M; Minagawa, Clarice Y; Telles, Evelise O; Garbuglio, Márcio A; Amaku, Marcos; Melville, Priscilla A; Dias, Ricardo A; Sakata, Sonia T; Benites, Nilson R

    2010-02-01

    Milk is the normal secretion of the mammary gland, practically free of colostrum and obtained by the complete milking of one or more healthy animals. Mastitis is an inflammatory process of the mammary gland and it may cause alterations in the milk. The present work aimed to verify whether it is possible, by means of the counts of microorganism in the bulk raw milk in four selective culture media, to establish a correlation with the occurrence of mastitis and therefore, to monitor this disease in bovine dairy herds. The following selective culture media were used: KF Streptococcus Agar, Edwards Agar, Baird-Parker Agar, Blood Agar plus potassium tellurite. Spearman's correlation coefficient was calculated in order to compare the occurrence of mastitis (percentage) in each herd with respective selective culture media counts of microorganisms in bulk raw milk. Thirty-six possibilities were analysed (Tamis and CMT-positive rates were compared with the log-transformed count in four selective culture media) and there was a negative correlation between Tamis 3 and the Baird-Parker Agar plate count. The total results of microbiological tests showed that there were three correlations of the counts in selective culture media. Fifty-two possibilities were analysed and there was a negative correlation between no-bacterial-growth mastitis rates and log10 of KF Streptoccocus Agar plate count and there were two positive correlations between coagulase-positive staphylococci and log10 of Baird-Parker Agar plate count and Blood Agar plus potassium tellurite plate count.

  18. New Media and the Construction of Tongliao City Culture%新媒介与通辽城市文化的构建磁

    Institute of Scientific and Technical Information of China (English)

    董拓

    2013-01-01

    City culture is focused on the process of meaning construction ,and it is belongs to the popular cul-ture .Nowadays ,popular culture mainly as a media culture .Reflections on the construction of city culture ,there are two main aspects :The characteristics of the new media and the importance of the new media on the city cul-ture of Tongliao .Media shaping the culture of the city ,and determines how city culture is expressed .New me-dia also play a special role in the culture of small and medium -sized cities .%  城市文化着重意义的构建,它的性质是大众文化。城市的大众文化在今日呈现为媒介文化。从新媒介的角度考察城市文化的构建,一是新媒介对于大众文化的重要性和特殊性,二是新媒介对于内蒙古自治区通辽市这城市文化的特殊性和重要性。媒介塑造着城市的文化,媒介的形式决定着城市文化如何被表达,媒介的倾向决定着城市文化如何被塑造。新媒介主导的城市文化在相对落后地区的中小城市同样有着它的特殊作用和重要意义。

  19. Huringiensis strategy to culture media design for the fermentation of bacillus thuringiensis

    OpenAIRE

    L. Beltrán; S. Díaz; Berdugo, C.; G. Buitrago; A Zamora; N. Moreno

    2012-01-01

    In this work was studied the culture medium for the Bacillus thuringiensis fermentation, the purpose was the production of biopesticide using as active ingredient native strains. The culture was developed in flasks of 1000 ml containing 100ml of culture medium, which was inoculated with 10ml of the bacteria, incubated at 29 ºC and 200 rpm. In this study we used an experimental design model for the strain HD1 of Bacillus thuringiensis, with glucose as carbon source, evaluating the concentratio...

  20. What does it mean to be a man today?: bloke culture and the media.

    Science.gov (United States)

    Hodgetts, Darrin; Rua, Mohi

    2010-03-01

    Psychologists have paid scant attention to the positive relationships and community contributions of working class men who are not in trouble, and have focused instead on men who are 'in trouble.' In addressing this oversight, we draw on insights from ethnographic observations, life narrative interviews, photographic techniques and media items, which have been compiled by 12 working class men from a shared community of practice in New Zealand. We illustrate how these men often appropriate aspects from contemporary media deliberations regarding what it means to be a man today in order to make sense of their own lives. The implications of participants' emphasis on friendship, support, familial obligations, and community participation are discussed in relation to the place of working class men in society.

  1. Modern Media Education Models

    Science.gov (United States)

    Fedorov, Alexander

    2011-01-01

    The author supposed that media education models can be divided into the following groups: (1) educational-information models (the study of the theory, history, language of media culture, etc.), based on the cultural, aesthetic, semiotic, socio-cultural theories of media education; (2) educational-ethical models (the study of moral, religions,…

  2. Promoting the Effect of the Qing Dynasty Imperial Garden Architectural Component Library on the Digitalization of Cultural Heritage

    Science.gov (United States)

    Jindan, C.; Junsong, Z.; Jiujun, Z.

    2015-08-01

    With the development of computer technology and practical verification, digital virtual technology has matured and is increasingly being widely applied to cultural heritage protection and research. With this advancement in technology, there is pressing need to simplify heritage-related puzzles. Thus the main question that has increasingly become the most central and fundamental problem in heritage digitalization work is how to choose the "proper technology" that provides support directly, accurately and rapidly for the research, protection and exchange of cultural heritage. Based on the principles of "authenticity" and "completeness" found in the Venice Charter in regards to dealing with cultural heritage; this paper proposes the concept of the component library which facilitates the improvement and efficiency of virtual reconstruction, provides a visual discussion platform for cultural heritage protection, virtual scene construction, accuracy assessment, and multi-space-time exhibition; thereby implementing the spirit of tolerance and respect found in the Nara Document on Authenticity. The paper further aims to illustrate the significance of the Qing dynasty imperial garden architectural component library for cultural heritage study and protection, the principles for virtual library construction, use and maintenance of the library, and classification approaches, and also provide some suggestions about making high quality 3D models and effective means for database integration.

  3. THE INFLUENCE OF CULTURE MEDIA ON ACETIC FERMENTATION WITH SELECTED Acetobacter STRAINS

    OpenAIRE

    MARIA CRISTIANA GARNAI

    2012-01-01

    We have systematically followed the efficiency of acetic fermentation, by cultivating 14 Acetobacter strains (previously isolated and identified), within a medium obtain out of ethanol and acetic acid, in various proportions, and utilizing corn extract (CE) as a nutrient. The purpose of the research was to determine the resistance of the studied Acetobacter strains related to the composition of the cultivation media (acidity and alcohol content of the medium), as well as following the dy...

  4. Morphological and Physicochemical Characterization of Agglomerates of Titanium Dioxide Nanoparticles in Cell Culture Media

    Directory of Open Access Journals (Sweden)

    Verónica Freyre-Fonseca

    2016-01-01

    Full Text Available Titanium dioxide nanoparticles (TiO2 NP are possible carcinogenic materials (2B-IARC and their toxicity depends on shape, size, and electrical charge of primary NP and on the system formed by NP media. The aim of this work was to characterize agglomerates of three TiO2 NP by evaluating their morphometry, stability, and zeta potential (ζ in liquid media and their changes with time. Sizes of agglomerates by dynamic light scattering (DLS resulted to be 10–50 times larger than those obtained by digital image analysis (DIA given the charged zone around particles. Fractal dimension (FD was highest for agglomerates of spheres and belts in F12K, and in E171 in FBS media. E171 and belts increased FD with time. At time zero, using water as dispersant FD was larger for agglomerates of spheres than for of E171. Belts suspended in water had the smallest values of circularity (Ci which was approximately unchanged with time. All dispersions had ζ values around −30 mV at physiological pH (7.4 and dispersions of NP in water and FBS showed maximum stability (Turbiscan Lab analysis. Results help in understanding the complex NP geometry-size-stability relationships when performing in vivo and in vitro environmental-toxicity works and help in supporting decisions on the usage of TiO2 NP.

  5. News Media as a Source of German Culture in the Classroom.

    Science.gov (United States)

    Michalski, John

    This article comments on the use of radio, television, films, newspaper, and other printed materials to supplement existing programs and textbooks. Specific references to television networks and newspapers illustrate the type of cultural material suggested for classroom use. (RL)

  6. Innovative Approaches Using Lichen Enriched Media to Improve Isolation and Culturability of Lichen Associated Bacteria

    National Research Council Canada - National Science Library

    Biosca, Elena G; Flores, Raquel; Santander, Ricardo D; Díez-Gil, José Luis; Barreno, Eva

    2016-01-01

    ... and for the description of new taxa. Our objective was to improve the recovery of lichen associated bacteria by developing novel isolation and culture approaches, initially using the lichen Pseudevernia...

  7. Book review: media and the rhetoric of body perfection:\\ud cosmetic surgery, weight loss and beauty in popular culture\\ud by Deborah Harris-Moore

    OpenAIRE

    Monson, Olivia

    2014-01-01

    Against the background of the so-called ‘obesity epidemic’, Media and the Rhetoric of Body Perfection critically examines the discourses of physical perfection that pervade Western societies, aiming to shed new light on the rhetorical forces behind body anxieties and extreme methods of weight loss and beautification. Drawing on interview material with cosmetic surgery patients and offering fresh analyses of various texts from popular culture, this book examines the ways in which the media cap...

  8. Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.

    Science.gov (United States)

    Ramos, Ana Carolina; Carvalhaes, Cecília Godoy; Cordeiro-Moura, Jhonatha Rodrigo; Rockstroh, Anna Carolina; Machado, Antonia Maria Oliveira; Gales, Ana Cristina

    2016-07-01

    In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producing Acinetobacter baumannii isolates grown on MacConkey agar medium. The other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.

  9. Effects of Cell Type and Culture Media on Interleukin-6 Secretion in Response to Environmental Particles

    OpenAIRE

    Veranth, John M; Cutler, N. Shane; Kaser, Erin G.; Reilly, Christopher A.; Yost, Garold S.

    2007-01-01

    Cultured lung cells provide an alternative to animal exposures for comparing the effects of different types of air pollution particles. Studies of particulate matter in vitro have reported proinflammatory cytokine signaling in response to many types of environmental particles, but there have been few studies comparing identical treatments in multiple cell types or identical cells with alternative cell culture protocols. We compared soil-derived, diesel, coal fly ash, titanium dioxide, and kao...

  10. O documento fotográfico : da mediação cultural à mediação técnica

    Directory of Open Access Journals (Sweden)

    Maria do Carmo Serén

    2011-01-01

    Full Text Available The media society is a global society, nomad and dedicated to recording, which, with the practiceof digital information, led to the definition of documentality (phenomenology of social objects. As a socialdocument, the photograph depends on and exists if people believe it exists as a document – as an intention,a will and an expression. Today, the photo-document is approached as form, sign and medium. If it is arecord that is apprehended with meaning it is sharable. With digital means, operations are done in any placeand at any time; the archiving of data preservation is delocalised (no direct appropriation takes place;reading requires the interface of a machine, breaking the imbrications and continuity between traditionalsupport and inscription, now subject to the programme’s formatting, which may place interpretation atrisk. Preservation only allows for migration to another devise, what remains is a logical structure, thecapacity to reproduce. Cultural measurement has been joined by technical measurement, which graduallytakes priority.

  11. Disordered eating, socio-cultural media influencers, body image, and psychological factors among a racially/ethnically diverse population of college women.

    Science.gov (United States)

    Quick, Virginia M; Byrd-Bredbenner, Carol

    2014-01-01

    This study examined disordered eating, socio-cultural media influencers, body image, and psychological factors among a large, racially/ethnically diverse sample of college women (n=1445; 58% White, 21% Asian, 11% Hispanic, 11% Black) who completed an online survey. Black women were significantly more satisfied with their weight and shape and had lower eating concerns, disinhibited eating, and emotional eating than all other racial/ethnic groups. Black women tended to have significantly higher levels of self-esteem, were less likely to compare their body to those of people in the media, felt less pressured to attain the physical appearance standard set by the media, and had less awareness of the societal appearance norms set by the media than other racial groups. Findings suggest that Black college women, independent of weight status, may be protected from disordered eating, negative body image, and societal media pressures. © 2013.

  12. Fast Swinnex Filtration (FSF): A fast and robust sampling and extraction method suitable for metabolomics analysis of cultures grown in complex media

    DEFF Research Database (Denmark)

    McCloskey, Douglas; Utrilla, Jose; Naviaux, Robert K.;

    2015-01-01

    of a suitable method for anaerobic cultures grown in complex media. Given that a vast majority of bacteria are facultative or obligate anaerobes that grow to low biomass density and need to be cultured in complex media, a suitable sampling and extraction strategy for anaerobic cultures is needed. In this work......Liquid chromatography tandem mass spectrometry (LC–MS/MS) provides a powerful means to analyze intracellular metabolism. A prerequisite to accurate metabolomics analysis using LC–MS/MS is a robust sampling and extraction protocol. One unaddressed area in sampling is a detailed examination......, we develop a fast-filtration method using pressuredriven Swinnex filters. We show that the method is fast enough to provide an accurate snapshot of intracellular metabolism, reduces matrix interference from the media to improve the number of compounds that can be detected, and is applicable...

  13. Viability and proliferation of L929, tumour and hybridoma cells in the culture media containing sericin protein as a supplement or serum substitute.

    Science.gov (United States)

    Cao, Ting-Ting; Zhang, Yu-Qing

    2015-09-01

    Cell cultures often require the addition of animal serum and other supplements. In this study, silk sericin, a bioactive protein, recovered from the waste of silk floss production was hydrolysed into three pepsin-degraded sericin peptides with different ranges of molecular mass. Normal animal cells, tumour cells and hybridoma cells were cultured systematically in FBS culture media containing sericin as a supplement or serum substitute. The culture test and microscopic observation of L929 cells showed that the smaller molecular weight of the degraded sericin is most suitable for cell culture. The cell culture results showed that with the degradation of sericin, for normal mouse fibroblast L929 cells, addition of 0.75 % sericin into FBS culture medium yields cell viability that is superior to FBS culture medium alone. When all serum was replaced by sericin, cell viability in the sericin medium could reach about one half of that in FBS medium. When in a medium containing a mixture of FBS: sericin (6:4, v/v), the cell culture effect is about 80 %. For the cultures of four tumour and one hybridoma cells, regardless of the molecular weight range, these degraded sericin peptides could substitute all serum in FBS media. The cell viability and proliferation of these tumour and hybridoma cells are equivalent or superior to that in FBS medium. In other words, cell viability and proliferation of these tumour and hybridoma cells in sericin media are more preferable to serum media. The mechanism of the sericin protein to promote cell growth and proliferation will be further investigated later.

  14. Media Convergence Requires the Elimination of Culture Gap%我国媒介融合需要消除文化隔膜

    Institute of Scientific and Technical Information of China (English)

    易法万

    2012-01-01

    Cultural integration is the essential requirement of media convergence and the overall economic efficiency of media industry. For the sake of media convergence, it's necessary to reform and innovate the journalism system, eliminate the culture gap between the traditional media and the new media, and take the popular path of development. It's also necessary to encourage the integration of media technology and the economic investment from all the society with harmonious culture orientation.%文化融合是媒介融合的本质要求,是传媒产业综合效益的内在保障。改革并创新新闻事业体制和新闻传播机制,消除传统媒体与新兴媒体之间的文化隔膜,走大众化的发展道路,媒介技术融合和全社会的经济投入以和谐的文化为导向,才是媒介融合潮流的正确选择。

  15. Nanoparticle dispersion in environmentally relevant culture media: a TiO{sub 2} case study and considerations for a general approach

    Energy Technology Data Exchange (ETDEWEB)

    Horst, Allison M., E-mail: ahorst@umail.ucsb.edu [University of California at Santa Barbara, Bren School of Environmental Science and Management (United States); Ji, Zhaoxia [UC Center for the Environmental Implications of Nanotechnology (UC CEIN) (United States); Holden, Patricia A. [University of California at Santa Barbara, Bren School of Environmental Science and Management (United States)

    2012-08-15

    Nanoparticle exposure in toxicity studies requires that nanoparticles are bioavailable by remaining highly dispersed in culture media. However, reported dispersion approaches are variable, mostly study-specific, and not transferable owing to their empirical basis. Furthermore, many published approaches employ proteinaceous dispersants in rich laboratory media, both of which represent end members in environmental scenarios. Here, a systematic approach was developed to disperse initially agglomerated TiO{sub 2} nanoparticles (Aeroxide Registered-Sign TiO{sub 2} P25, Evonik, NJ; primary particle size range 6.4-73.8 nm) in oligotrophic culture medium for environmentally relevant bacterial toxicity studies. Based on understanding particle-particle interactions in aqueous media and maintaining environmental relevance, the approach involves (1) quantifying the relationship between pH and zeta potential to determine the point of zero charge of select nanoparticles in water; (2) nominating, then testing and selecting, environmentally relevant stabilizing agents; and (3) dispersing via 'condition and capture' whereby stock dry powder nanoparticles are sonicated in pre-conditioned (with base, or acid, plus stabilizing agent) water, then diluted into culture media. The 'condition and capture' principle is transferable to other nanoparticle and media chemistries: simultaneously, mechanically and electrostatically, nanoparticles can be dispersed with surrounding stabilizers that coat and sterically hinder reagglomeration in the culture medium.

  16. The Transnational Identity of European Film Festival. New Media and Cultural Branding Employed at Transylvania International Film Festival

    Directory of Open Access Journals (Sweden)

    Ileana Nicoleta SĂLCUDEAN

    2017-07-01

    Full Text Available European film festival venues are explored in their relation to transnationalism, a “supranational sphere”, as well as with political and economic implications (Acciari, 2014. The international film festivals are seen as cosmopolitan spaces (Chan 2011, 253, yet, the new morphology of film festivals - perceived as "public spheres" or as new objects of historical research - bring a new light on film festivals and the theory of culture and visual discourse, especially with the new reconfiguration of festivals in Europe, the insertion of new technologies and new opportunities to explore local identity. The article examines the cultural determinants and the new vocabulary of visual discourse, exploring the implied questions regarding national identity vs. European identity, and the possibility of building a cultural city/ country branding. With a case study on Transylvania International Film Festival, I attempt an inquiry of three interconnected aspects employed in exploring film festivals and their transnational dimension: the impact of the new media on the audience, the challenge of identity and the possibility to create a city/ country branding.

  17. Analysis of adult otitis media: polymerase chain reaction versus culture for bacteria and viruses.

    Science.gov (United States)

    Liederman, E M; Post, J C; Aul, J J; Sirko, D A; White, G J; Buchman, C A; Ehrlich, G D

    1998-01-01

    Recent studies using the polymerase chain reaction (PCR) have identified bacterial and viral genomic sequences in culture-negative pediatric middle ear effusions. To evaluate this technique in adults, 19 effusions were analyzed to compare bacterial and viral culture and PCR detection of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and adenovirus. Effusions from 4 subjects positive for human immunodeficiency virus (HIV) were analyzed by PCR for HIV virus. Three of 19 effusions were culture-positive for bacteria, and 0 of 19 for viruses. Fifteen of 19 effusions were PCR-positive for bacterial genomic sequences, and 0 of 19 for adenovirus. Thirteen of 15 PCR-positive specimens demonstrated S pneumoniae, 5 of 15 H influenzae, and 0 of 13 M catarrhalis. All 4 effusions from HIV-positive subjects were PCR-positive for HIV. No effusion was culture-positive and PCR-negative. These results confirm that culture-negative middle ear effusions contain genomic sequences from bacterial pathogens. Finding of HIV RNA and DNA in effusion from HIV-positives suggests replicating virus in this fluid.

  18. Variability in biochemical components of the mussel (Mytilus galloprovincialis) cultured after Prestige oil spill.

    Science.gov (United States)

    Peteiro, Laura G; Labarta, Uxío; Fernández-Reiriz, María José

    2007-05-01

    The biochemical composition (proteins, carbohydrates, glycogen, total lipids and lipid classes) of the mussel Mytilus galloprovincialis was investigated during an experimental culture using mussel seed from areas with different degree of exposure to the Prestige oil spill. The aim of the study was to identify alterations in the biochemical composition of mussel seed from natural populations commonly used in Galicia for mussel raft culture that might be linked to previous oil exposure. We have selected three mussel seed populations from Pindo, Miranda and Redes, that were characterised in a previous study according to the oil exposure three months after the spill. These populations were transplanted to a raft culture system in the Ría de Ares-Betanzos where our experimental culture followed standard commercial techniques from March 2003 to February 2004. Mussels from Pindo (characterised as the most affected area by the oil spill) showed marked differences in lipid composition with regard to other populations in the content of triacylglycerols, (PRedes (designed as reference area) which may reflect that Miranda mussels were not affected or only hardly affected by the spill. With the exception of the onset of the culture, biochemical composition showed similar patterns in all mussel populations. Then, the fact of being cultured in a common environment seemed to be more responsible for the long-term variability in the energetic reserve than the origin of the populations or their previous biochemical status.

  19. Comparison of New and Traditional Culture-Dependent Media for Enumerating Foodborne Yeasts and Molds.

    Science.gov (United States)

    Beuchat, Larry R; Mann, David A

    2016-01-01

    Fifty-six foods and food ingredients were analyzed for populations of naturally occurring yeasts and molds using Petrifilm rapid yeast and mold (RYM) count plates, Petrifilm yeast and mold (YM) count plates, dichloran rose bengal chloramphenicol (DRBC) agar plates, acidified potato dextrose agar (APDA) plates, and dichloran 18% glycerol (DG18) agar plates. Colonies were counted after incubating plates for 48, 72, and 120 h at 25°C. Of 56 foods in which either yeasts or molds were detected on at least one medium incubated for 120 h, neither yeasts nor molds were detected in 55.4, 73.2, 21.4, 19.6, and 71.4% of foods plated on the five respective media and incubated for 48 h; 10.7, 14.3, 3.6, 1.8, and 19.6% of foods were negative after 72 h, and 3.6, 1.8, 0, 0, and 0% of foods were negative after 120 h. Considering all enumeration media, correlation coefficients were 0.03 to 0.97 at 48 h of incubation; these values increased to 0.75 to 0.99 at 120 h. Coefficients of variation for total yeasts and molds were as high as 30.0, 30.8, and 27.2% at 48, 72, and 120 h, respectively. The general order of performance was DRBC = APDA > RYM Petrifilm > YM Petrifilm ≥ DG18 when plates were incubated for 48 h, DRBC > APDA > RYM Petrifilm > YM Petrifilm ≥ DG18 when plates were incubated for 72 h, and DRBC > APDA > RYM Petrifilm > YM Petrifilm > DG18 when plates were incubated for 120 h. Differences in performance among media are attributed to the diversity of yeasts and molds likely to be present in test foods and differences in nutrient, pH, and water activity requirements for resuscitation of stressed cells and colony development.

  20. THE INFLUENCE OF CULTURE MEDIA ON ACETIC FERMENTATION WITH SELECTED Acetobacter STRAINS

    Directory of Open Access Journals (Sweden)

    MARIA CRISTIANA GARNAI

    2012-06-01

    Full Text Available We have systematically followed the efficiency of acetic fermentation, by cultivating 14 Acetobacter strains (previously isolated and identified, within a medium obtain out of ethanol and acetic acid, in various proportions, and utilizing corn extract (CE as a nutrient. The purpose of the research was to determine the resistance of the studied Acetobacter strains related to the composition of the cultivation media (acidity and alcohol content of the medium, as well as following the dynamics of the acetic fermentation by calculating the practical yield. The research led to optimal variants which may be industrially exploited in order to obtain vinegar.