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Sample records for cucurbita moschata duchesne

  1. Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel

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    El Zawane Kamarudin; Qamar Uddin Ahmed; Zuvairea Nazren Mohd Sirajudin; Ahmad Jalal Khan Chowdhury

    2014-01-01

    Objective: To explore the in vitro antibacterial potential of the peel of Cucurbita moschata Duchesne (tropical pumpkin) (C. moschata) against human pathogenic bacteria. Methods:In the present study, dichloromethane (DCM), methanol (MEOH) and aqueous extracts of C. moschata peel were examined for in vitro antibacterial potency against eight bacterial strains i.e. Bacillus cereus, Burkholderia cepacia, Escherichia coli, Enterococcus faecalis, Staphyloccocus aureus, Pseudomonas aerugenosa, Vibrio alginolyticus, Vibrio parahaemolyticus using Kirby-Bauer disk diffusion susceptibility and broth micro-dilution methods. Results: DCM extract of pumpkin peel exhibited the maximum zone of inhibition against Staphyloccocus aureus (21 mm) whereas aqueous extract of pumpkin peel revealed the least zone of inhibition against Escherichia coli (8 mm). MEOH extract gave maximum zone of inhibition against Pseudomonas aerugenosa (19 mm). Broth micro-dilution method showed minimum inhibitory concentration for the DCM extract against Burkholderia cepacia at 6.25 mg/mL. The minimum bactericidal concentrations were also determined to know the nature of all extracts. DCM and MEOH extracts exhibited bactericidal nature to all bacterial strains except for the Vibrio alginolyticus. The minimum bactericidal concentrations values exhibited bactericidal nature ranging from 3.12 mg/mL to 100.00 mg/mL. The screening of antimicrobial properties of different extracts of C. moschata peel revealed that the DCM extract possessed good antimicrobial efficacy compared to MEOH and aqueous extracts. Conclusions: Peel of C. moschata possesses antibacterial compounds and could be potential source for a new class of antibiotics.

  2. Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne peel

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    El Zawane Kamarudin

    2014-02-01

    Full Text Available Objective: T o explore the in vitro antibacterial potential of the peel of Cucurbita moschata D uchesne ( tropical pumpkin ( C. moschata against human pathogenic bacteria. Methods: I n the present study, dichloromethane ( DCM , methanol ( MEOH and aqueous extracts of C. moschata peel were examined for in vitro antibacterial potency against eight bacterial strains i.e. Bacillus cereus, Burkholderia cepacia, Escherichia coli, Enterococcus faecalis, Staphyloccocus aureus, Pseudomonas aerugenosa, Vibrio alginolyticus, Vibrio parahaemolyticus using K irby- B auer disk diffusion susceptibility and broth micro-dilution methods. Results: DCM extract of pumpkin peel exhibited the maximum zone of inhibition against Staphyloccocus aureus ( 21 mm whereas aqueous extract of pumpkin peel revealed the least zone of inhibition against Escherichia coli ( 8 mm . MEOH extract gave maximum zone of inhibition against Pseudomonas aerugenosa ( 19 mm . B roth micro-dilution method showed minimum inhibitory concentration for the DCM extract against Burkholderia cepacia at 6 . 25 mg/m L . T he minimum bactericidal concentrations were also determined to know the nature of all extracts. DCM and MEOH extracts exhibited bactericidal nature to all bacterial strains except for the Vibrio alginolyticus. T he minimum bactericidal concentrations values exhibited bactericidal nature ranging from 3 . 12 mg/m L to 100 . 00 mg/m L . T he screening of antimicrobial properties of different extracts of C. moschata peel revealed that the DCM extract possessed good antimicrobial efficacy compared to MEOH and aqueous extracts. Conclusions: P eel of C. moschata possesses antibacterial compounds and could be potential source for a new class of antibiotics.

  3. Evaluation of phytochemicals, antioxidant and burn wound healing activities of Cucurbita moschata Duchesne fruit peel

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    Roodabeh Bahramsoltani

    2017-07-01

    Full Text Available Objective(s: Cucurbita moschata Duchesne (pumpkin is a well-known plant with several pharmacological effects. The aim of the present study was to assess burn wound healing activity of C. moschata peel extract (CE. Also, standardized CE was assessed for antioxidant activity and antibacterial effects against major pathogens of burns. Materials and Methods: Healing properties of topical preparation of 10% and 20% concentrations of CE were assessed on second degree burn in rats during a 14-day period as well as histological studies, total antioxidant power, lipid peroxidation and total thiol content of skin tissue samples. Results: Radical scavenging IC50 and ferric-reducing antioxidant power value were 4.015±0.20 mg/ml and 142.63±2.65 mmol Fe2+/g, respectively. Total mucilage content was 13.8%. The optimal results were obtained by 20% CE that showed 90.80±5.86 % wound closure and tissue repair as well as significant reduction of tissue oxidative stress biomarkers. Histological analyses confirmed wound healing activity of pumpkin peel extract. Conclusion: Considering the high mucilage content of the plant, providing a moist environment for wound, C. moschata peel extract could be a natural remedy for treatment of burns. Further clinical studies are suggested to confirm C. moschata peel extract as a wound healing agent.

  4. Seed oil content and fatty acid composition in a genebank collection of Cucurbita moschata Duchesne and C. argyrosperma C. Huber

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    Data on intraspecific variability for seed oil content, fatty acid composition and seed oil characteristics in Cucurbita moschata and C. argyrosperma are lacking in the scientific literature. We examined 528 genebank accessions of C. moschata and 166 accessions of C. argyrosperma - that included mem...

  5. 南瓜矮生基因Bu的比较定位%Comparative Mapping of the Dwarf Gene Bu from Tropical Pumpkin (Cucurbita moschata Duchesne)

    Institute of Scientific and Technical Information of China (English)

    王深浩; 李海真; 张忠华; 贺俊; 贾长才; 张帆; 黄三文

    2011-01-01

    A segregating BC6F2 population was developed using a dwarf mutant of Cucurbita moschata Duch. as donor and the vine C. maxima Duch. ‘Mengri’ as recurrent parent. Using the segregating population, the gene Bu was comparatively mapped on chromosome 5 of cucumber. An intron-flanking marker IF3629 was found to be tightly linked to the Bu gene, with a genetic distance of 1.0 cM. The marker IF3629 will not only be useful in markers-assisted selection for dwarf plant, but also provide a starting point toward positional isolation of the dwarf Bu gene in squash.%以中国南瓜矮生突变体为供体亲本,以印度蔓生南瓜为轮回亲本,构建了BC6F2分离群体.利用黄瓜基因组序列,将南瓜矮生基因Bu比较定位至黄瓜5号染色体,并开发了一个新的PCR标记IF3629,该标记与矮生基因Bu连锁遗传距离为1.0 cM.该标记不仅可以用于分子标记辅助选择育种,而且为Bu基因的克隆奠定了基础.

  6. Qualitative and quantitative differences in carotenoid composition among Cucurbita moschata, Cucurbita maxima, and Cucurbita pepo.

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    Azevedo-Meleiro, Cristiane H; Rodriguez-Amaya, Delia B

    2007-05-16

    Squashes and pumpkins are important dietary sources of carotenoids worldwide. The carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. In the present work, the carotenoid composition of squashes and pumpkins currently marketed in Campinas, Brazil, were determined by HPLC-DAD, complemented by HPLC-MS for identification. Cucurbita moschata 'Menina Brasileira' and C. moschata 'Goianinha' had similar profiles, with beta-carotene and alpha-carotene as the major carotenoids. The hybrid 'Tetsukabuto' resembled the Cucurbita pepo 'Mogango', lutein and beta-carotene being the principal carotenoids. Cucurbita maxima 'Exposição' had a different profile, with the predominance of violaxanthin, followed by beta-carotene and lutein. Combining data from the current study with those in the literature, profiles for the Cucurbita species could be observed. The principal carotenoids in C. moschata were beta-carotene and alpha-carotene, whlereas lutein and beta-carotene dominate in C. maxima and C. pepo. It appears that hydroxylation is a control point in carotenoid biosynthesis.

  7. New phenolic glycosides from the seeds of Cucurbita moschata.

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    Li, Fa-Sheng; Dou, De-Qiang; Xu, Liang; Chi, Xiao-Feng; Kang, Ting-Guo; Kuang, Hai-Xue

    2009-07-01

    Two new phenolic glycosides were isolated from the seeds of Cucurbita moschata. Their structures were elucidated as (2-hydroxy)phenylcarbinyl 5-O-benzoyl-beta-D-apiofuranosyl(1-->2)-beta-D-glucopyranoside (1) and 4-beta-D-(glucopyranosyl hydroxymethyl)phenyl 5-O-benzoyl-beta-D-apiofuranosyl(1-->2)-beta-D-glucopyranoside (2) on the basis of spectroscopic analysis and chemical evidence.

  8. Antioxidative activity of water soluble polysaccharide in pumpkin fruits (Cucurbita maxima Duchesne).

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    Nara, Kazuhiro; Yamaguchi, Akira; Maeda, Naomi; Koga, Hidenori

    2009-06-01

    We evaluated the antioxidative activity of a water soluble polysaccharide fraction (WSP) from pumpkin fruits (Cucurbita maxima Duchesne). In the WSP, DPPH radical scavenging and superoxide dismutase-like activity increased depending on the total sugar content. Furthermore, the WSP can serve as an inhibitor of ascorbic acid oxidation. The efficacy was also affected by the total sugar content.

  9. SSR-based genetic linkage map of Cucurbita moschata and its synteny with Cucurbita pepo.

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    Gong, L; Pachner, M; Kalai, K; Lelley, T

    2008-11-01

    The first SSR-based genetic linkage map of Cucurbita moschata was created by integrating the maps of two F2 populations with one common parent developed from the crosses Waltham Butternut (WB) x Nigerian Local (NL) and ZHOU (a hull-less type) x WB. The integrated C. moschata map comprises 205 SSR markers and two morphological traits (Gr and n). The map is composed of 27 linkage groups with a marker density of 7 cM. Comparing the C. moschata map with the published Cucurbita pepo map, we found a high level of macrosynteny. Seventy-two of 76 common SSR markers between C. moschata and C. pepo were located in homologous linkage groups. These markers in general have conserved orders and similar genetic distances; they represent orthologous loci. A reference map based on these SSRs was obtained. No major chromosomal rearrangement between the two species could be detected at present, although four SSR markers were mapped in nonhomologous linkage groups. The comparative alignment of SSR markers did not provide any indication of a possible ancient polyploid origin of the species. The comparative mapping of C. moschata and C. pepo reported here will be useful for further studies on Cucurbit evolution, gene isolation, and breeding work.

  10. Isolation of pectin from pumpkin (Cucurbita moschata, L.) by acid hydrolysis;Isolamento de pectina de abobora (Cucurbita moschata, L.) por hidrolise acida

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    Souza, Jose R.R.; Ricardo, Nagila M.P.S.; Paula, Regina C.M. de; Feitosa, Judith P.A., E-mail: judith@dqoi.ufc.b [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica. Lab. de Polimeros; Brito, Edy S. [EMBRAPA Agroindustria Tropical, Fortaleza, CE (Brazil)

    2009-07-01

    Pumpkin (Cucurbita moschata, L.) constitutes an excellent source of carotenoids, precursors of vitamin A. Besides, it also that constitutes also a great natural source of low-cost pectin. Pectin is a heterogeneous complex polysaccharide found in the primary cell wall of most plants and its effect on health is receiving increasing interest from the scientific community. In this work, high-methoxy pectin was obtained from pumpkin (Cucurbita moschata, L.) through the acid hydrolysis methodology. The pectin obtained was characterized by FTIR, NMR {sup 1}H, GPC and rheology. (author)

  11. Cucumber (Cucumis sativus L.) and kabocha squash (Cucurbita moschata Duch).

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    Nanasato, Yoshihiko; Tabei, Yutaka

    2015-01-01

    We established improved methods for Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) and kabocha squash (Cucurbita moschata Duch). Vacuum infiltration of cotyledonary explants with Agrobacterium suspension enhanced the Agrobacterium infection efficiency in the proximal regions of explants. Wounding treatment was also essential for kabocha squash. Cocultivation on filter paper wicks suppressed necrosis of explants, keeping regeneration efficacy. Putative transgenic plants were screened by kanamycin resistance and green fluorescent protein (GFP) fluorescence. These putative transgenic plants grew normally and T1 seeds were obtained, and stable integration and transmission of the transgene in T1 generations were confirmed by Southern hybridization and PCR. The average transgenic efficiency for cucumber and kabocha squash was 11.9 ± 3.5 and 9.2 ± 2.9 %, respectively.

  12. Chemical and Physicochemical Characterization of Winter Squash (Cucurbita moschata D.

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    Noelia JACOBO-VALENZUELA

    2011-05-01

    Full Text Available Winter squash cv ‘Cehualca’ (Cucurbita moschata Duchense is a seasonal crop that has been used as food and animal feed. The objective of the present study was to characterize physical, chemical and physicochemical properties of the winter squash cv ‘Cehualca’. Morphological, chemical and physicochemical analyses were performed, including fiber, carotenoids, phenolic and mineral contents in the winter squash. The morphological analysis showed that the squash ‘Cehualca’ did not have a homogeneous morphology. Data about their physical and physicochemical characteristics showed large variability. Also, high content of carotenoids and dietary fiber was observed in squash. The oil and total phenolic content was low in comparison with other fruits. The mineral content exceeded the values recommended to meet the nutritional needs of consumers, except for sodium (both in pulp and shell, potassium and magnesium in the shell.

  13. The sterols of Cucurbita moschata ("calabacita") seed oil.

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    Rodriguez, J B; Gros, E G; Bertoni, M H; Cattaneo, P

    1996-11-01

    From the sterol fraction of seed oil from commercial Cucurbita moschata Dutch ("calabacita") delta 5 and delta 7 sterols having saturated and unsaturated side chain were isolated by chromatographic procedures and characterized by spectroscopic (1H and 13C-nuclear magnetic resonance, mass spectrometry) methods. The main components were identified as 24S-ethyl 5 alpha-cholesta-7,22E-dien-3 beta-ol (alpha-spinasterol); 24S-ethyl 5 alpha-cholesta-7,22E,25-trien-3 beta-ol (25-dehydrochondrillasterol); 24S-ethyl 5 alpha-cholesta-7,25-dien-3 beta-ol; 24R-ethyl-cholesta-7-en-3 beta-ol (delta 7-stigmastenol) and 24-ethyl-cholesta-7, 24(28)-dien-3 beta-ol (delta 7,24(28)-stigmastadienol).

  14. [Allelopathic effects of cultured Cucurbita moschata root exudates].

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    Li, Min; Ma, Yongqin; Shui, Junfeng

    2005-04-01

    By using the techniques of tissue culture, bio-assay and laboratory analysis, this paper studied the effects of the allelopathic chemicals from pumpkin (Cucurbita moschata) roots on the seed germination and seedling growth of pumpkin, wheat (Triticum aestivum), and radish (Raphanus sativus). The pumpkin root was cultured on a sterile B5 media, and the concentrations of macro- and microelements, organic supplements and hormones in the media were adjusted by using an orthogonal design. After culturing, the culture media was filtered and used in a bioassay to test the autotoxicity and allelopathic effects. The results showed that the pumpkin had both autotoxic and allelopathic effects, and the media having been used to culture the pumpkin roots contained the chemicals that significantly inhibited the seedling growth of wheat and radish. The allelopathic effect decreased when the culture media was diluted. The production of allelochemicals seemed to be related to the growth rate of the pumpkin roots. When the root growth was rapid, the concentration of allelochemicals was high. The allelopathic effect was stronger on radish than on wheat. The optimum concentrations of macro- and microelements, vitamins and hormones for culturing pumpkin root were determined, and the effect of pumpkin root nutrition on the production of allelochemicals was tested. The results indicated that pumpkin root nutrition had a significant effect on the production of allelochemicals.

  15. Evaluación de poblaciones de zapallo (Cucurbita moschata por caracteres de importancia agroindustrial Evaluation of squash (Cucurbita moschata populations for significant agribusiness traits

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    Magda Piedad Valdés Restrepo

    2010-01-01

    Full Text Available Se evaluó el potencial agroindustrial de siete poblaciones de zapallo (Cucurbita moschata derivadas de la recombinación de genotipos de alto desempeño en caracteres asociados con rendimiento y calidad de fruto. Los análisis de varianza para los resultados obtenidos en los semestres 2008-A y 2008-B permitieron detectar diferencias altamente significativas (P The agribusiness potential of seven squash (Cucurbita moschata populations derived of the recombination of genotypes of high performing in characters associated with yield and quality of the fruit was evaluated. The Anova analyses for semesters 2008-A and 2008-B allowed to detect highly significant differences (P < 0.01 among populations, for all the variables, except for color flesh fruit in 2008-A. The populations 1 and 5 showed a relative permanence in dry matter content in two cycles of genetic recombination.

  16. In vitro effects of Cucurbita moschata seed extracts on Haemonchus contortus.

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    Marie-Magdeleine, C; Hoste, H; Mahieu, M; Varo, H; Archimede, H

    2009-04-01

    This study was carried out to evaluate the in vitro effect of Cucurbita moschata seed against the parasitic nematode of small ruminants Haemonchus contortus. Three extracts (aqueous, methanolic and dichloromethane) of C. moschata seed were tested in vitro on four developmental stages of H. contortus using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition (LMI) assay and adult worm motility (AWM) assay. The highly significant (P90% for each extract) and the negative effect of the dichloromethane and methanolic extracts on adult worm motility (inhibition of motility >59.2% after 24h of incubation) compared to the negative controls, suggest anthelmintic properties of C. moschata seed against H. contortus. The active principles responsible for the activity could be secondary metabolites such as amino acid compounds or terpenoid compounds present in the extracts.

  17. In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata

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    Marta Valdez-Melara

    2009-11-01

    Full Text Available An efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP, 2,4-dichlorophenoxyacetic acid (2,4-D and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56% and cotyledons from seedlings (70% of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally. Rev. Biol. Trop. 57 (Suppl. 1: 119-127. Epub 2009 November 30.

  18. Structures of new phenolic glycosides from the seeds of Cucurbita moschata.

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    Li, Fa-Sheng; Xu, Jing; Dou, De-Qiang; Chi, Xiao-Feng; Kang, Ting-Guo; Kuang, Hai Xue

    2009-04-01

    A new phenolic glycoside and three known compounds were isolated from the seeds of Cucurbita moschata. The structures of the new compound was elucidated as phenylcarbinyl 5-O-(4-hydroxy)benzoyl-beta-D-apiofuranosyl (1-->2)-beta-D-glucopyranoside on the basis of spectroscopic analysis and chemical evidence. Three known compounds were identified as 1-O-benzyl[5-O-benzoyl-beta-D-apiofuranosyl(1-->2)]-beta-D-glucopyranoside 2, cucurbitosides C 3 and A 4, by comparison of the spectral data with reported data. Compound 2 was isolated from this plant for the first time.

  19. Development of microsatellite markers from an enriched genomic library of pumpkin (Cucurbita moschata L.

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    Nuchjaree Watcharawongpaiboon

    2007-09-01

    Full Text Available Development of microsatellite markers in Pumpkin (Cucurbita moschata L. was performed using the biotin-streptavidin enrichment procedure. One hundred and thirty three clones were randomly selected. After sequence analysis of 31 randomly picked positive colonies, 100% of the colonies were found to contain microsatellite sequences, and 9 primer pairs were designed. Five of the primers tested could amplify pumpkins DNA and can be used for genetic purity testing of the commercial hybrids. This paper reports the first isolation and utilization of microsatellite markers in pumpkin.

  20. In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata

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    Marta Valdez-Melara

    2009-11-01

    Full Text Available An efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP, 2,4-dichlorophenoxyacetic acid (2,4-D and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56% and cotyledons from seedlings (70% of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally. Rev. Biol. Trop. 57 (Suppl. 1: 119-127. Epub 2009 November 30.En este estudio se desarrolló un método eficiente y reproducible para la regeneración de líneas puras de la planta tropical Cucurbita moschata mediante la vía de embriogénesis somática. Además se investigó acerca de la influencia del genotipo, transplante, y la concentración de N6-benzylaminopurina (BAP, 2,4-diclorofenoxyacetico ácido (2,4-D y 2,4,5-triclorofenoxyacetico ácido (2,4,5-T en la inducción de embriogénesis somática. Los callos embriogenéticos viables fueron producidos de embriones zigóticos (53-56% y cotiledones de semillas (70% de C. moschata cv. Sello de Oro cultivados en un medio de inducción de callos (CIM suplementado con 0.5 mg/l o 3.5 mg

  1. Molecular Replacement Studies of Cucurmosin from Cucurbita Moschata: Structure Homology with Trichosanthin

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    SHI Xiao-Li(石小莉); Edward X. Zhou; YE Xiao-Ming(叶晓明); Edward J. Meehan; CHEN Ming-Huang(陈明晃); Liqing Chen(陈荔清)

    2003-01-01

    High diffraction quality crystals of cucurmosin, a type Ⅰ ribosome inactivating protein isolated from the sarcocarp of Cucurbita moschata (pumpkin), have been grown under newly optimised conditions. With in-house rotating anode X-ray source, these crystals diffract to 1.65 A resolution which is much higher than that of the previously reported crystals that diffracted only to 3 A resolution. The crystals belong to space group P212121 with cell parameters a = 41.5, b = 58.4 and c = 99.3 A. Molecular replacement studies indicate that the cucurmosin structure is homologous to trichosanthin. The initial structural model has been obtained and the model fitting/refinement is in progress.

  2. Evaluación de poblaciones de zapallo (Cucurbita moschata por caracteres de importancia agroindustrial

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    Magda Piedad Valdés Restrepo

    2010-01-01

    Full Text Available Se evaluó el potencial agroindustrial de siete poblaciones de zapallo (Cucurbita moschata derivadas de la recombinación de genotipos de alto desempeño en caracteres asociados con rendimiento y calidad de fruto. Los análisis de varianza para los resultados obtenidos en los semestres 2008-A y 2008-B permitieron detectar diferencias altamente significativas (P < 0.01 entre poblaciones, para todas las variables, con excepción de color de fruto en el semestre 2008-A. Las poblaciones 1 y 5 mostraron consistencia para el contenido de materia seca en los dos ciclos de recombinación genética.

  3. Cellulase-assisted extraction of polysaccharides from Cucurbita moschata and their antibacterial activity.

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    Qian, Zhi-Gang

    2014-01-30

    In this study, cellulase-assisted extraction of water soluble polysaccharides from pumpkin (Cucurbita moschata) and their antibacterial activity were investigated. The polysaccharides yield was monitored during the extraction process. The optimum extraction conditions were determined as follows: time, 40 min; temperature, 55°C; pH, 4.5; and cellulase amount, 4,000 U/g. The extracts were centrifuged, filtered, proteins removed by Sevag method, concentrated to approximately 15% (w/v), precipitated with 5 volumes of absolute ethanol, freeze-dried, and pulverized to yield a water soluble powder of pumpkin polysaccharides (PP). The sugar content of the product was 68.3%, and the yield was 17.34% (w/w), respectively. The PP had high antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli at the concentration of 100 mg/mL.

  4. Evaluación de poblaciones de zapallo (Cucurbita moschata por caracteres de importancia agroindustrial

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    Vallejo Cabrera Franco Alirio

    2010-03-01

    Full Text Available Se evaluó el potencial agroindustrial de siete poblaciones de zapallo (Cucurbita moschata derivadas de la recombinación de genotipos de alto desempeño en caracteres asociados con rendimiento y calidad de fruto. Los análisis de varianza para los resultados obtenidos en los semestres 2008-A y 2008-B permitieron detectar diferencias altamente significativas (P < 0.01 entre poblaciones, para todas las variables, con excepción de color de fruto en el semestre 2008-A. Las poblaciones 1 y 5 mostraron consistencia para el contenido de materia seca en los dos ciclos de recombinación genética.

  5. Improved postharvest quality in patagonian squash ( Cucurbita moschata) coated with radiation depolymerized chitosan

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    Pugliese, Maria Alicia; Goitia, Maria Teresa; Yossen, Mariana; Cifone, Norma; Agulló, Enrique; Andreucetti, Noemi

    2011-12-01

    Different molecular weight chitosans were evaluated on the decay of coated Anquito squashes ( Cucurbita moschata) as well as the maintenance of the fruit quality along five storage months. The original chitosan (Mw=391 kDa, 83% DD), was depolymerized by gamma radiation. Apart from chain scission, other chemical changes were not detected by FTIR or UV-vis analyses. The molecular weight characterization of chitosans was done by size exclusion chromatography with dual light scattering and concentration detection (SEC-MALLS-RI). The coating effectiveness was evaluated on the following parameters: fungal decay incidence, weight loss, firmness, total reducing sugar, soluble solid, flesh color, carotene content, pH and titratable acidity. No sign of fungal decay was observed in squashes coated with 122 and 56 kDa chitosans, which were also the most effective treatments in reducing the weight loss. The chitosan with Mw=122 kDa was also the best treatment considering firmness, internal aspect, sugar and carotene content. Then, radiation degraded chitosan was better in C. moschata preservation than the original chitosan.

  6. Pumpkin (Cucurbita moschata) fruit extract improves physical fatigue and exercise performance in mice.

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    Wang, Shih-Yi; Huang, Wen-Ching; Liu, Chieh-Chung; Wang, Ming-Fu; Ho, Chin-Shan; Huang, Wen-Pei; Hou, Chia-Chung; Chuang, Hsiao-Li; Huang, Chi-Chang

    2012-10-09

    Pumpkin (Cucurbita moschata) is a popular and nutritious vegetable consumed worldwide. The overall purpose of this study was to evaluate the effects of C. moschata fruit extract (CME) on anti-fatigue and ergogenic functions following physiological challenges. Male ICR mice from four groups designated vehicle, CME-50, CME-100 and CME-250, respectively (n = 8 per group in each test) were orally administered CME for 14 days at 0, 50, 100 and 250 mg/kg/day. The anti-fatigue activity and exercise performance were evaluated using exhaustive swimming time, forelimb grip strength, as well as levels of plasma lactate, ammonia, glucose, and creatine kinase after an acute swimming exercise. The resting muscular and hepatic glycogen was also analyzed after 14-day supplementation with CME. Trend analysis revealed that CME treatments increased grip strength. CME dose-dependently increased 5% body weight loaded swimming time, blood glucose, and muscular and hepatic glycogen levels. CME dose-dependently decreased plasma lactate and ammonia levels and creatine kinase activity after a 15-min swimming test. The mechanism was relevant to the increase in energy storage (as glycogen) and release (as blood glucose), and the decrease of plasma levels of lactate, ammonia, and creatine kinase. Therefore, CME may be potential for the pharmacological effect of anti-fatigue.

  7. Pumpkin (Cucurbita moschata Fruit Extract Improves Physical Fatigue and Exercise Performance in Mice

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    Chia-Chung Hou

    2012-10-01

    Full Text Available Pumpkin (Cucurbita moschata is a popular and nutritious vegetable consumed worldwide. The overall purpose of this study was to evaluate the effects of C. moschata fruit extract (CME on anti-fatigue and ergogenic functions following physiological challenges. Male ICR mice from four groups designated vehicle, CME-50, CME-100 and CME-250, respectively (n = 8 per group in each test were orally administered CME for 14 days at 0, 50, 100 and 250 mg/kg/day. The anti-fatigue activity and exercise performance were evaluated using exhaustive swimming time, forelimb grip strength, as well as levels of plasma lactate, ammonia, glucose, and creatine kinase after an acute swimming exercise. The resting muscular and hepatic glycogen was also analyzed after 14-day supplementation with CME. Trend analysis revealed that CME treatments increased grip strength. CME dose-dependently increased 5% body weight loaded swimming time, blood glucose, and muscular and hepatic glycogen levels. CME dose-dependently decreased plasma lactate and ammonia levels and creatine kinase activity after a 15-min swimming test. The mechanism was relevant to the increase in energy storage (as glycogen and release (as blood glucose, and the decrease of plasma levels of lactate, ammonia, and creatine kinase. Therefore, CME may be potential for the pharmacological effect of anti-fatigue.

  8. Improved postharvest quality in patagonian squash (Cucurbita moschata) coated with radiation depolymerized chitosan

    Energy Technology Data Exchange (ETDEWEB)

    Pugliese, Maria Alicia; Goitia, Maria Teresa [Laboratorio de Investigaciones Basicas Aplicadas en Quitina, Departamento de Quimica, Universidad Nacional del Sur. Avenida Alem 1253, B8000CPB Bahia Blanca (Argentina); Yossen, Mariana [Instituto de Desarrollo Tecnologico para la Industria Quimica (INTEC), CONICET-Universidad Nacional del Litoral, Ruta Nacional 168-Paraje ' El Pozo' , 3000 Santa Fe (Argentina); Cifone, Norma; Agullo, Enrique [Laboratorio de Investigaciones Basicas Aplicadas en Quitina, Departamento de Quimica, Universidad Nacional del Sur. Avenida Alem 1253, B8000CPB Bahia Blanca (Argentina); Andreucetti, Noemi, E-mail: andreuce@criba.edu.ar [Laboratorio de Radioisotopos, Departamento de Quimica, Universidad Nacional del Sur, Avenida Alem 1253, B8000CPB Bahia Blanca (Argentina)

    2011-12-15

    Different molecular weight chitosans were evaluated on the decay of coated Anquito squashes (Cucurbita moschata) as well as the maintenance of the fruit quality along five storage months. The original chitosan (Mw=391 kDa, 83% DD), was depolymerized by gamma radiation. Apart from chain scission, other chemical changes were not detected by FTIR or UV-vis analyses. The molecular weight characterization of chitosans was done by size exclusion chromatography with dual light scattering and concentration detection (SEC-MALLS-RI). The coating effectiveness was evaluated on the following parameters: fungal decay incidence, weight loss, firmness, total reducing sugar, soluble solid, flesh color, carotene content, pH and titratable acidity. No sign of fungal decay was observed in squashes coated with 122 and 56 kDa chitosans, which were also the most effective treatments in reducing the weight loss. The chitosan with Mw=122 kDa was also the best treatment considering firmness, internal aspect, sugar and carotene content. Then, radiation degraded chitosan was better in C. moschata preservation than the original chitosan. - Highlights: > Original Chitosan was radiation depolymerized producing chitosans with lower molecular weights. > Gamma-irradiated chitosans only exhibit chain scission. > SEC-MALLS-RI chromatography is a useful tool in molecular weight analysis. > Depolymerized chitosans were the best in maintaining the quality and the storage life of coated squashes.

  9. Chemical composition and antioxidant activity of an acidic polysaccharide extracted from Cucurbita moschata Duchesne ex Poiret.

    Science.gov (United States)

    Yang, Xingbin; Zhao, Yan; Lv, You

    2007-06-13

    A simple and sensitive high-performance capillary electrophoresis (HPCE) method was designed for quantitative analysis of the component monosaccharides of an acidic polysaccharide extracted from pumpkin. In this method, the polysaccharide was hydrolyzed into component monosaccharides with 2.0 M trifluoroacetic acid at 100 degrees C for 6 h and then labeled with 1-phenyl-3-methyl-5-pyrazolone, and subsequently the labeled monosaccharide derivatives were separated by HPCE. As a result, glucose (21.7%) and glucuronic acid (18.9%) were identified to be the main component monosaccharides, followed by galactose (11.5%), arabinose (9.8%), xylose (4.4%), and rhamnose (2.8%). Furthermore, the pumpkin polysaccharide was also demonstrated to effectively inhibit the H2O2-caused decrease of cell viability, lactate dehydrogenase leakage, and malondialdehyde formation, and also reduced the H2O2-caused decline of superoxide dismutase activity and glutathione depletion in cultured mouse peritoneal macrophages, indicating that pumpkin polysaccharide possessed significant cytoprotective effect and antioxidative activity.

  10. Crystallization and preliminary crystallographic study of cucurmosin, a ribosome-inactivating protein from the sarcocarp of Cucurbita moschata.

    Science.gov (United States)

    Chen, M; Ye, X; Cai, J; Lin, Y

    2000-05-01

    Cucurmosin, a ribosome-inactivating protein purified from pumpkin, the sarcocarp of Cucurbita moschata, has been crystallized using polyethylene glycol as a precipitant. The crystals belong to space group P2(1)2(1)2(1) and have unit-cell parameters a = 41.91, b = 59. 48, c = 98.78 A. There is one molecule in the asymmetric unit. The diffraction data to 3.0 A resolution were collected on a MAR Research image-plate detector.

  11. Genetic Correlations and Path Analysis in Butternut Squash Cucurbita moschata Duch / Correlaciones Genéticas y Análisis de Sendero en Zapallo Cucurbita moschata Duch

    Directory of Open Access Journals (Sweden)

    Sanín Ortiz Grisales

    2014-12-01

    Full Text Available Abstract. A genetic correlation and path analysis was carried out for yield and fruit quality components of diallel crosses of butternut squash Cucurbita moschata Duch, using openpollinated introductions, S0 and inbred lines S1 and S2 at two sites: Candelaria and Buga, located in the Department of Valle delCauca, Colombia. A split-plot experimental design with arranged, random blocks and four replicates was used. The average fruit weight presented positive genetic correlations with the total production per plant in all three generations and at both test sites. The total plant dry matter and total production per plant presented a high genetic correlation, and the fruit dry matter was inversely correlated with the total carotene. The total carotene and fruit color were independent. The path analysis confirmed high genetic correlations between the number of fruits per plant, average fruitweight, and total plant dry matter, with direct, positive effects, that were significantly greater than zero for the variables of number of fruits per plant and average fruit weight in regards to the total plant dry matter. The total production per plant expressed a direct, negative effect on the total plant dry matter. The fruit dry matter presented an indirect, negative effect on the total plant dry matter through the average fruit weight and an indirect, positive effect through the total production per plant. / Resumen. Se estudiaron las correlaciones genéticas y el análisis de sendero para los componentes del rendimiento y calidad del fruto de zapallo Cucurbita moschata Duch, en cruzamientos dialélicos entre introducciones de libre polinización S0 y sus líneas S1 y S2 en dos localidades, Candelaria y Buga, ubicadas en el Departamento del Valle del Cauca, Colombia. Se usó un diseño experimental en parcelas divididas, arregladas en bloques al azar con cuatro repeticiones. El peso promedio del fruto presentó correlación gen

  12. An overview of winter squash (Cucurbita maxima Duch. and pumpkin (Cucurbita moschata Duch. growing in Turkey

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    Ahmet Balkaya

    2015-06-01

    Full Text Available Cucurbita L. species of the pumpkin and winter squash are grown all over the world. Winter squash and pumpkin are two of the most important Cucurbit vegetable crops in Turkey. Turkey is one of the important diversity areas, for the cultivated cucurbits because of their adaptation to diverse ecological conditions as a result of both natural selection and also the selection by farmers. Farmers have maintained the local population of winter squash and pumpkin, which are mainly sold in local markets. Only one improved cultivar of the winter squash is currently grown commercially in Turkey. It is a traditional vegetable often grown in small gardens. In this contribution, the last status of winter squash and pumpkin production in Turkey, the growing techniques and problems of these winter squash and pumpkin species, their genetic collection and characterization, and the utilization of the presented species in Turkey are examined.

  13. Diversidad genética de germoplasma colombiano de zapallo (Cucurbita moschata Dúchesne Exp. Prior

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    Vallejo C. F. A.

    2004-12-01

    Full Text Available Se colectaron, caracterizaron morfológicamente y evaluaron agronómicamente 133 accesiones de Cucurbita moschata en Colombia (departamentos del Cauca, Valle del Cauca, Risaralda, Quindío, Caldas, Antioquia, Bolívar, Magdalena y Atlántico, las cuales presentaron alta diversidad en tamaño, forma y color de fruto. Los descriptores pubescencia de la hoja, color mancha de la hoja, días a floración masculina, días a floración femenina, espesor de pulpa, tamaño de semilla, forma de margen de semilla, ancho de fruto y peso promedio de fruto permitieron la diferenciación de grupos. Se encontró un índice de diversidad de 62% entre las accesiones colectadas en los departamentos de la región andina con las de la Costa Atlántica. Ninguno de los grupos presentó índices de similaridad del 100%. ABSTRACT This study was carried at Candelaria (Colombia, from August 2001 to July 2003. This study aimed at evaluating, by multivariate analyses techniques, the genetic diversity of 133 accesions, collected from distinct areas of regions of Colombia. The experimental design was of randomized complete blocks with four replications. The data (average were collected from three plants per plot. Thirteen nine traits were evaluated, 9 vegetatives, 24 reproductives and 1 related to susceptibility to stress. The data were analysed using the herarquical method of WARD and were transformed into a complete disjunctive table, where the modalities of each character is a new variable. The analysis revealed five cluster, with to R2 = 97% and diversity index 62%. The most important characteristics to explain the variability were: days to first female and male flower, fruit width, fruit weight, flesh thickness, leaf pubescence dorsal and ventral surface, colour of leaf spots and seed size. The 5, 7, 47 , 109 and 28 accesion might be recommended for breeding programs. Keywords: Cucurbita moschata, Germplasm, Genetic resources, plant breeding, Morphology

  14. Cucurbita moschata Duch. and its active component, β-carotene effectively promote the immune responses through the activation of splenocytes and macrophages.

    Science.gov (United States)

    Kim, Hee-Yun; Nam, Sun-Young; Yang, Shi-Young; Kim, Hyung-Min; Jeong, Hyun-Ja

    2016-10-01

    Cucurbita moschata Duch. has long been used for traditional health food in many countries. However, to enhance the immune system of Cucurbita moschata Duch. and its major component, β-carotene is not clear. Here, we determined the immune enhancement effect of Cucurbita moschata Duch. and β-carotene in mouse splenocytes and RAW 264.7 macrophage cell line. We prepared baked Cucurbita moschata Duch. (Sweetme Sweet Pumpkin(TM), SSP) and steamed Cucurbita moschata Duch. (SC). Splenocytes isolated from the spleen of BALB/c mice were treated with SSP, SC, and β-carotene for 24 h. RAW 264.7 cells were stimulated with recombinant interferon-γ (rIFN-γ) for 6 h before treatment with SSP, SC, or β-carotene. SSP, SC and β-carotene significantly up-regulated the proliferation of splenocyte and mRNA expression of KI-67. The levels of interleukin-2 and IFN-γ were up-regulated by SSP, SC, or β-carotene in the splenocytes. SC and β-carotene also increased the levels of tumor necrosis factor-α (TNF-α) in the splenocytes. In addition, SSP, SC, or β-carotene significantly increased the levels of TNF-α through the nuclear translocation of the nuclear factor-κB and phosphorylation of IκBα in the rIFN-γ-primed RAW 264.7 cells. These data indicate that Cucurbita moschata Duch. and β-carotene may have an immune-enhancing effect through the production of Th1 cytokines by activation of splenocytes and macrophages.

  15. Carotenoid composition and vitamin A value of an Argentinian squash (Cucurbita moschata).

    Science.gov (United States)

    González, E; Montenegro, M A; Nazareno, M A; López de Mishima, B A

    2001-12-01

    The carotenoid composition of butternut squash (Cucurbita moschata) cultivated in the province of Santiago del Estero, Argentina, was determined. The main carotenoids isolated were identified as beta-carotene (beta,beta-carotene), alpha-carotene (beta,epsilon-carotene), and lutein (beta,epsilon-carotene-3,3'-diol) and the minor carotenoids, as phytofluene (7,8,11,12,7',8'-hexahydro-psi,psi-carotene), zeta-carotene (7,8,7',8'-tetrahydro-psi,psi-carotene), neurosporene (7,8-dihydro-psi,psi-carotene), violaxanthin (5,6,5',6'- diepoxy-5,6,5',6'-tetrahydro-beta,beta-carotene-3,3'-diol) and neoxanthin (5,6-epoxy-6,7-didehydro-5,6,5',6'-tetrahydro-beta,beta- carotene-3,5,3'-triol). In some samples, 5,6,5',6'-beta-carotene diepoxide, (5,6,5',6'-diepoxy-5,6,5',6'-tetrahydro-beta,beta-carotene) and flavoxanthin (5,8-epoxy-5,8-dihydro-beta,epsilon-carotene-3,3'-diol) were detected. The presence of cis-isomers of beta,beta-carotene was also detected by HPLC. The vitamin A value obtained was 432 micrograms RE/100 g fresh sample, which indicates that this vegetable is an important source of provitamin A.

  16. Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata).

    Science.gov (United States)

    Wu, Hao; Zhu, Junxiang; Diao, Wenchao; Wang, Chengrong

    2014-11-26

    An efficient ultrasound-assisted enzymatic extraction (UAEE) of Cucurbita moschata polysaccharides (CMCP) was established and the CMCP antioxidant activities were studied. The UAEE operating parameters (extraction temperature, ultrasonic power, pH, and liquid-to-material ratio) were optimized using the central composite design (CCD) and the mass transfer kinetic study in UAEE procedure was used to select the optimal extraction time. Enzymolysis and ultrasonication that were simultaneously conducted was selected as the UAEE synergistic model and the optimum extraction conditions with a maximum polysaccharide yield of 4.33 ± 0.15% were as follows: extraction temperature, 51.5 °C; ultrasonic power, 440 W; pH, 5.0; liquid-to-material ratio, 5.70:1 mL/g; and extraction time, 20 min. Evaluation of the antioxidant activity in vitro suggested that CMCP has good potential as a natural antioxidant used in the food or medicine industry because of their high reducing power and positive radical scavenging activity for DPPH radical.

  17. Effect of storage on physicochemical, microbial and antioxidant properties of pumpkin (Cucurbita moschata candy

    Directory of Open Access Journals (Sweden)

    Sabeera Muzzaffar

    2016-12-01

    Full Text Available Pumpkin (Cucurbita moschata is highly nutritious and antioxidant-rich vegetable widely grown all over the world. Present study reports the effect of storage on physicochemical, microbial, and antioxidant properties of pumpkin candy. Pumpkin and its candy were analyzed for the physicochemical characteristics like moisture content, ash, total soluble solids (TSS, titrable acidity, total sugar, reducing sugar, and color. Beta-carotene and vitamin-C content of pumpkin and its candy were also studied. Antioxidant properties like 1, 1-Diphenyl-2-picrylhydrazyl (DPPH, ferric reducing antioxidant power (FRAP, total phenolic content (TPC, reducing power, and lipid peroxidation of methanolic extracts of pumpkin and processed candy were evaluated. During storage, a significant increase in TSS while a non-significant increase in titrable acidity, reducing and total sugars was observed. Beta-carotene, vitamin C, color, and antioxidant properties (DPPH, FRAP, TPC, reducing power, and lipid peroxidation also showed a non-significant decrease during storage at ambient temperature. Microbial load of pumpkin candy (1.74–3.2 log cfu/g suggested that candies were safe for human consumption during storage. Hence, candy preparation from pumpkin could be an effective method for preservation of pumpkin and retention of its bioactive components.

  18. [Immunocytological localization of IAA in the parenchyma cell and vascular elements in the graft union of Cucurbita pepo/Cucurbita moschata at the early developmental stage].

    Science.gov (United States)

    Wang, Y Q; Han, J; Jia, W S; Du, Z

    2000-06-01

    Immuno-gold localization of IAA in cells of the graft union in the explant internode graft of Cucurbita pepo/Cucurbita moschata were investigated with electron microscopy. In parenchyma cells near the graft union, the gold particles were mainly accumulated in nucleus, plastid and endoplasmic reticulum, while no gold particles was detected in Golgi body, mitochondrion, cell wall and vacuoles. In the differentiating xylem element, the gold particles were labeled in secondary wall and cytoplasm. In the sieve element gold particles were found in the sieve plate, sieve pore and cytoplasm. There was a dense label of the gold particles in the companion cell. The role of IAA in the differentiation of the vascular elements was discussed.

  19. 13-Hydroxy-9Z,11E,15E-octadecatrienoic acid from the leaves of Cucurbita moschata.

    Science.gov (United States)

    Bang, Myun-Ho; Han, Jae-Taek; Kim, Hae Yeong; Park, Young-Doo; Park, Chang-Ho; Lee, Kang-Ro; Baek, Nam-In

    2002-08-01

    A new unsaturated hydroxy fatty acid was isolated from the leaves of Cucurbita moschata through repeated silica gel column chromatography and chemical methods. The structure of the new fatty acid was determined as 13-hydroxy-9,11,15-octadecatrienoic acid on the basis of several spectral data including 2D-NMR. The stererostructures of double bonds were determined to be 9Z, 11E and 15E by coupling patterns of related proton signals in the 1H-NMR and NOESY experiments.

  20. Secagem de fatias de abóboras (Cucurbita moschata, L. por convecção natural e forçada Drying of pumpkin (Cucurbita moschata, L. slices by natural and forced convection

    Directory of Open Access Journals (Sweden)

    Soraia Vilela Borges

    2008-12-01

    Full Text Available A abóbora (Cucurbita moschata, L. é uma importante fonte de provitamina A, de baixo custo, e sob a forma desidratada oferece diferentes opções de utilização e consumo. Secagens por convecção natural e forçada foram comparadas quanto ao grau de secagem atingido e encolhimento, em função da temperatura, velocidade de ar e dimensões do produto. Os resultados obtidos mostraram que o uso de fatias com volume de 6,25 cm³ em secador por convecção forçada a 50 °C e à velocidade de 5,5 x 10-4 ms-1 resultaram em produtos de menor encolhimento, sendo recomendadas estas condições.Besides its low price, Pumpkin (Cucurbita moschata, L. is an important source of provitamin-A, and when dehydrated it offers different options of utilizations and consumption. Natural and forced convection drying were compared according to the drying degree shrinkage as a function of temperature, and air velocity and product dimensions. The obtained results showed that slices with the volume of 6.25 cm³ in forced convection oven at 50 °C and at the velocity of 5.5 x 10-4 ms-1 resulted in a lower shrinkage products, so these conditions were recommended.

  1. Characterization of bioactive compounds, antioxidant activity and minerals in landraces of pumpkin (Cucurbita moschata cultivated in Southern Brazil

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    Daniela PRIORI

    2016-01-01

    Full Text Available Abstract The objective of this work was to evaluate the genetic variability for the synthesis of bioactive compounds and minerals in pumpkin (Cucurbita moschata landraces. Total phenolic compounds, carotenoids, antioxidant activity and minerals were evaluated in 10 accessions of Cucurbita moschata landraces from the Genebank of Embrapa Temperate Agriculture (Pelotas - RS, Brazil. Twenty plants of each accession were cultivated in the field during the spring/summer of 2013/2014. After harvesting of mature fruits, the seeds were discarded and opposite longitudinal portions of the pulp were manually prepared for analysis of the bioactive compounds. For the determination of minerals, pumpkin samples were frozen in plastic bags, and after freeze-dried and milled. All analysis were performed in triplicate. The data obtained showed high genetic variability for the synthesis of phenolic compounds, carotenoids, antioxidant activity and minerals. The accessions C52, C81, C267 e C389 showed high levels of antioxidants and minerals, being recommended for use in pumpkin breeding programs. The accessions C52 and C389 are promising, especially because they present the highest levels of total carotenoids.

  2. Carboxylesterase-involved metabolism of di-n-butyl phthalate in pumpkin (Cucurbita moschata) seedlings.

    Science.gov (United States)

    Lin, Qingqi; Chen, Siyuan; Chao, Yuanqing; Huang, Xiongfei; Wang, Shizhong; Qiu, Rongliang

    2017-01-01

    Uptake and accumulation by plants is a significant pathway in the migration and transformation of phthalate esters (PAEs) in the environment. However, limited information is available on the mechanisms of PAE metabolism in plants. Here, we investigated the metabolism of di-n-butyl phthalate (DnBP), one of the most frequently detected PAEs, in pumpkin (Cucurbita moschata) seedlings via a series of hydroponic experiments with an initial concentration of 10 mg L(-1). DnBP hydrolysis occurred primarily in the root, and two of its metabolites, mono-n-butyl phthalate (MnBP) and phthalic acid (PA), were detected in all plant tissues. The MnBP concentration was an order of magnitude higher than that of PA in shoots, which indicated MnBP was more readily transported to the shoot than was PA because of the former's dual hydrophilic and lipophilic characteristics. More than 80% of MnBP and PA were located in the cell water-soluble component except that 96% of MnBP was distributed into the two solid cellular fractions (i.e., cell wall and organelles) at 96 h. A 13-20% and 29-54% increase of carboxylesterase (CXE) activity shown in time-dependent and concentration-dependent experiments, respectively, indicated the involvement of CXEs in plant metabolism of DnBP. The level of CXE activity in root subcellular fractions was in the order: the cell water-soluble component (88-94%) > cell wall (3-7%) > cell organelles (3-4%), suggesting that the cell water-soluble component is the dominant locus of CXE activity and also the domain of CXE-catalyzed hydrolysis of DnBP. The addition of triphenyl phosphate, a CXE inhibitor, led to 43-56% inhibition of CXE activity and 16-25% increase of DnBP content, which demonstrated the involvement of CXEs in plant metabolism of DnBP. This study contributes to our understanding of enzymitic mechanisms of PAE transformation in plants.

  3. Inbreeding and Gene Action in Butternut Squash (Cucurbita moschata Seed Starch Content / Endocría y Acción Génica para el Contenido Almidón en Semilla de Zapallo (Cucurbita moschata

    Directory of Open Access Journals (Sweden)

    Sanin Ortiz Grisales

    2014-03-01

    Full Text Available Abstract. The effect of inbreeding and gene action on butternutsquash (Cucurbita moschata Duch. seed production and seedstarch content was evaluated at two locations in the departmentof Valle del Cauca, Colombia, using six accessions (S0 and their inbred lines S1 and S2. Significant differences were found between the accessions and inbred lines, but not between localities. The seed production and seed starch content showed no significant effects of inbreeding depression. An additive-type gene action predominated, suggesting that recurrent selection could be the best strategy to increase the frequencies of genes promoting seed production and seed starch content. / Resumen. Se evaluó el efecto de la endocría y la acción génica en la producción de semilla y en el contenido de almidón en la semilla de zapallo (Cucurbita moschata Duch. en dos localidades del departamento del Valle del Cauca, Colombia, utilizando seis accesiones (S0 y sus respectivas líneas endocriadas S1 y S2. Se detectaron diferencias significativas entre accesiones y líneas endocriadas pero no entre localidades. La producción de semilla y el contenido de almidón no presentaron efectos significativos de depresión endogámica. Predominó la acción génica de tipo aditivo, sugiriendo que la selección recurrente podría ser la mejor estrategia para incrementar las frecuencias de los genes que favorecen la producción de semilla y contenido de almidón en la semilla.

  4. Kinetic mechanism and order of substrate binding for sn-glycerol-3-phosphate acyltransferase from squash (Cucurbita moschata).

    Science.gov (United States)

    Hayman, Matthew W; Fawcett, Tony; Slabas, Antoni R

    2002-03-13

    sn-Glycerol-3-phosphate acyltransferase (G3PAT, EC 2.3.1.15), a component of glycerolipid biosynthesis, is an important enzyme in chilling sensitivity in plants. The three-dimensional structure of the enzyme from squash (Cucurbita moschata), without bound substrate, has been determined [Turnbull et al. (2001) Acta Crystallogr. D 57, 451-453; Turnbull et al. (2001) Structure 9, 347-353]. Here we report the kinetic mechanism of plastidial G3PAT from squash and the order of substrate binding using acyl-acyl carrier protein (acyl-ACP) substrates. The reaction proceeds via a compulsory-ordered ternary complex with acyl-ACP binding before glycerol-3-phosphate. We have also determined that the reaction will proceed with C(4:0)-CoA, C(6:0)-CoA and C(12:0)-ACP substrates, allowing a wider choice of acyl groups for future co-crystallisation studies.

  5. Mutagenesis of squash (Cucurbita moschata) glycerol-3-phosphate acyltransferase (GPAT) to produce an enzyme with altered substrate selectivity.

    Science.gov (United States)

    Hayman, M W; Fawcett, T; Schierer, T F; Simon, J W; Kroon, J T; Gilroy, J S; Rice, D W; Rafferty, J; Turnbull, A P; Sedelnikova, S E; Slabas, A R

    2000-12-01

    In an attempt to rationalize the relationship between structure and substrate selectivity of glycerol-3-phosphate acyltransferase (GPAT, 1AT, EC 2.3.1.15) we have cloned a number of cDNAs into the pET overexpression system using a PCR-based approach. Following assay of the recombinant enzyme we noted that the substrate selectivity of the squash (Cucurbita moschata) enzyme had altered dramatically. This form of GPAT has now been crystallized and its full three-dimensional structure elucidated. Since we now have two forms of the enzyme that display different substrate selectivities this should provide a powerful tool to determine the basis of the selectivity changes. Kinetic and structural analyses are currently being performed to rationalize the changes which have taken place.

  6. Effects of different pruning ways on the sugar-decreasing matters of pumpkin (Cucurbita moschata) fruits during the ripeness

    Institute of Scientific and Technical Information of China (English)

    NI Shujun; JIANG Xinmei; LIU Shouwei; YU Xihong

    2007-01-01

    This experiment used pumpkin Longyuanlixiang as material to research the effect of different pruning ways with one branch, two branches, and three branches on the sugar-decreasing matters during the ripeness of pumpkin (Cucurbita moschata) fruits. The results showed that pectin contents of pumpkin fruits during the ripeness all increased at first and then dropped in every treatment. The pectin contents under the treatments of one branch and two branches reached the high apex after pollinated for 30 d as well as for 35 d under the treatment of three branches. Pentose and chromium contents of fruits had the same trend under different pruning ways and increased during the ripenes. Three kinds of sugar-decreasing matters contents under treatment of two branches were bigger than treatment of one branch, while treatment of three branches was the lowest.

  7. Agrobacterium-mediated transformation of kabocha squash (Cucurbita moschata Duch) induced by wounding with aluminum borate whiskers.

    Science.gov (United States)

    Nanasato, Yoshihiko; Konagaya, Ken-ichi; Okuzaki, Ayako; Tsuda, Mai; Tabei, Yutaka

    2011-08-01

    An efficient genetic transformation method for kabocha squash (Cucurbita moschata Duch cv. Heiankogiku) was established by wounding cotyledonary node explants with aluminum borate whiskers prior to inoculation with Agrobacterium. Adventitious shoots were induced from only the proximal regions of the cotyledonary nodes and were most efficiently induced on Murashige-Skoog agar medium with 1 mg/L benzyladenine. Vortexing with 1% (w/v) aluminum borate whiskers significantly increased Agrobacterium infection efficiency in the proximal region of the explants. Transgenic plants were screened at the T(0) generation by sGFP fluorescence, genomic PCR, and Southern blot analyses. These transgenic plants grew normally and T(1) seeds were obtained. We confirmed stable integration of the transgene and its inheritance in T(1) generation plants by sGFP fluorescence and genomic PCR analyses. The average transgenic efficiency for producing kabocha squashes with our method was about 2.7%, a value sufficient for practical use.

  8. Correlaciones fenotípicas, genéticas y ambientales en Cucurbita moschata Duch. Ex Poir

    Directory of Open Access Journals (Sweden)

    Baena García D.

    2005-03-01

    Full Text Available El estudio estimó las correlaciones fenotípicas, genéticas y ambientales entre doce caracteres agronómicos en dos dialélicos de zapallo (uno entre cinco variedades y otro entre cinco líneas S1, originadas de las anteriores. Se usó un diseño en bloques completos al azar con 15 tratamientos (5 progenitores + 10 cruzamientos F1 para cada dialélico y cinco repeticiones. Los resultados señalaron mayor estimación de las correlaciones (en magnitud y significancia estadística a favor del dialélico de líneas S1. Las correlaciones genéticas fueron superiores a las fenotípicas y las ambientales. La producción por planta (PFP presentó las correlaciones parciales fenotípicas y genéticas más altas con el peso por fruto (PPF y el número de frutos por planta (NFP (rPF y rPG > 0.80. El PPF y NFP pueden usarse como criterios de selección para la obtención de cultivares de altos rendimientos en C. moschata. ABSTRACT Phenotypic, genotypic and environmental correlations in Cucurbita moschata Duch. Ex Poir. The study evaluated phenotypic, genotypic and environmental correlations among 12 agronomy characters, in two set diallelic from five parents each one. The first set from five open pollinated varieties and the second one from five self-pollinated lines S1, obtained from the previous varieties. The two experiments were in completely randomized blocks with five replications. A higher estimation of correlation (in magnitude and statistical significance was observed in favor of the S1 lines diallelic. The genetic correlations were higher than the phenotypic and environmental correlations. The plant yield (PFP showed the highest partial phenotypic and genetic correlations with the fruit weight (PPF and fruits per plant (NFP (rPF and rPG > 0.80. It is suggested to use the PPF and NFP as selection criteria for breeding of C. moschata for development of high yield cultivars. Key words: Calabaza (cucurbita, genetic correlation, agronomic

  9. VARIASI KOMBINASI TEPUNG LABU KUNING (Cucurbita moschata D.) DAN TEPUNG AZOLLA (Azolla pinnata R.br.) PADA KECERAHAN WARNA IKAN KOI (Cyprinus carpio L.)

    OpenAIRE

    Utami, Diah Ayu Tri

    2014-01-01

    Warna memegang peranan penting dalam menentukan kualitas ikan koi (Cyprinus carpio L.), bahkan dipandang sebagai komponen penting dalam proses seleksi ikan hias. Permasalahan yang sering timbul adalah memudarnya warna ikan bila dipelihara dalam jangka waktu yang lama. Penelitian ini menambahkan tepung labu kuning (Cucurbita moschata D.) dan tepung Azolla (Azolla pinnata R.Br.) ke dalam pakan buatan. Dengan penambahan tepung labu kuning dan tepung Azolla ini memungkinkan ikan memperoleh lebih ...

  10. Caracterización molecular de introducciones colombianas de zapallo Cucurbita moschata Molecular characterization of colombian introductions of squash Cucurbita moschata

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    Javier A. Restrepo S

    2008-01-01

    Full Text Available Se realizó la caracterización molecular mediante el polimorfismo en la longitud de fragmentos amplificados (AFLP de 121 introducciones de C. moschata, del Banco de Germoplasma del Programa de Investigación en Hortalizas de la Universidad Nacional de Colombia Sede Palmira, provenientes de ocho departamentos de Colombia. Los datos AFLP se evaluaron utilizando análisis de correspondencia múltiple (ACM, distancia genética, análisis genético con el programa TFPGA y el método de agrupamiento UPGMA. La diversidad genética de estas introducciones fue alta y estuvo de acuerdo con la diversidad morfoagronómica estudiada previamente. Los valores Fst indicaron que existe estructura genética entre la mayoría de las introducciones. La mayoría de la variación genética entre las introducciones se atribuyó a variación entre individuos dentro de cada departamento.The molecular characterization was carried out by means of the amplified fragment length polymorphism (AFLP of 121 introductions of C. moschata, of the germoplasm bank of the Vegetables Research Program of the Universidad Nacional de Colombia campus Palmira, originating from eight departments of Colombia . The AFLP data were evaluated utilizing multiple correspondence analysis (MCA, genetic distance, genetic analysis with the TFPGA program and the UPGMA cluster analysis. The genetic diversity of these introductions was high and was according with the morphoagronomic diversity previously studied. The Fst values indicated that exist genetic structure between most of the introductions. The most of the genetic variation between the introductions corresponded to variations between them inside each department.

  11. Molecular characterization of colombian introductions of squash Cucurbita moschata Caracterización molecular de introducciones colombianas de zapallo Cucurbita moschata

    Directory of Open Access Journals (Sweden)

    Restrepo Javier

    2008-03-01

    Full Text Available The molecular characterization was carried out by means of the amplified fragment length polymorphism (AFLP of 121 introductions of C. moschata, of the germoplasm bank of the Vegetables Research Program of the Universidad Nacional de Colombia campus Palmira, originating from eight departments of Colombia. The AFLP data were evaluated utilizing multiple correspondence analysis (MCA, genetic distance, genetic analysis with the TFPGA program and the UPGMA cluster analysis. The genetic diversity of these introductions was high and was according with the morphoagronomic diversity previously studied. The Fst values indicated that exist genetic structure between most of the introductions. The most of the genetic variation between the introductions corresponded to variations between them inside each department.Se realizó la caracterización molecular mediante el polimorfismo en la longitud de fragmentos amplificados (AFLP de 121 introducciones de C. moschata, del Banco de Germoplasma del Programa de Investigación en Hortalizas de la Universidad Nacional de Colombia Sede Palmira, provenientes de ocho departamentos de Colombia. Los datos AFLP se evaluaron utilizando análisis de correspondencia múltiple (ACM, distancia genética, análisis genético con el programa TFPGA y el método de agrupamiento UPGMA. La diversidad genética de estas introducciones fue alta y estuvo de acuerdo con la diversidad morfoagronómica estudiada previamente. Los valores Fst indicaron que existe estructura genética entre la mayoría de las introducciones. La mayoría de la variación genética entre las introducciones se atribuyó a variación entre individuos dentro de cada departamento.

  12. 5个南瓜自交系光合特性的比较%Comparison of Photosynthetic Characteristics in Five Pumpkin(Cucurbita moschata Duch) Inbred Lines

    Institute of Scientific and Technical Information of China (English)

    刘振威; 李新峥; 孙丽; 李贞霞

    2007-01-01

    南瓜为一年生葫芦科南瓜属植物,是一种古老而又普通的蔬菜种类,在世界范围内广泛栽培。主要栽培种有3个:南瓜(Cucurbita moschata Duch),又叫中国南瓜;笋瓜(Cucurbita maxima Duch),又叫印度南瓜;西葫芦(Cucurbita pepo L.),又叫美洲南瓜。随着科技的进步和对南瓜研究的深入,南瓜在中国人民的膳食结构中被赋予了新的功能,它不仅是人们喜食的菜肴,而且是重要的营养保健食品。

  13. Variabilidad en frutos y semillas de Cucurbita moschata Duch. y Cucurbita argyrosperma subsp. sororia L.H. Bailey Merrick & D.M. Bates: Fruit and seeds variability in butternut squash Cucurbita moschata Duch. and Cucurbita argyrosperma subsp. sororia L. H.Bailey Merrick & D.M. Bates

    Directory of Open Access Journals (Sweden)

    Magda Piedad Valdés Restrepo

    2014-09-01

    Full Text Available En las cucurbitáceas (zapallo se presenta una gama amplia de variantes para el color de la semilla, desde blanco a café, que pueden tener significado evolutivo y de distribución geográfica. Se evaluó la variabilidad en semillas de 295 introducciones de Cucurbita moschata Duch. y 99 de Cucurbita argyrosperma subsp. sororia, todas procedentes de libre polinización. El análisis de conglomerados evidenció 11 grupos para cada una de las especies, las cuales expresaron alta variabilidad entre y dentro de las introducciones para los caracteres porcentaje de extracto etéreo en semilla (EE, peso de semilla por fruto (PSPF y número de frutos por planta (NFP. En C. moschata se destacaron dos grupos (5 y 9 por presentar altos promedios para producción por planta (PPL, peso de semilla por fruto (PSPF y extracto etéreo por planta (EEPP, con coeficientes de variación (CV entre 52% y 93%. En C. argyrosperma subsp. sororia se destacaron para los mismos caracteres los grupos 5 y 11, con un CV entre 53% y 79%. Los elevados coeficientes de variación evidenciaron amplia variabilidad entre y dentro de introducciones para los caracteres de importancia agronómica y agroindustrial. Teniendo en cuenta el análisis de agrupamiento y el índice de selección ponderado (ISP se seleccionaron las seis mejores introducciones en cada especie.

  14. Effect of squash seed meal (Cucurbita moschata on broiler performance, sensory meat quality, and blood lipid profile

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    YM Aguilar

    2011-12-01

    Full Text Available In this experiment, 240 Cobb-500® broilers reared from1 to 49 days, and distributed according to a completely experimental randomized design with four treatments of four replicates each in order to evaluate the effect of the dietary inclusion of 0, 33, 66 or 100 g/kg of squash seed meal (SSM (Cucurbita moschata on the performance, carcass yield, serum lipid profile and sensory meat quality of broilers. Significant differences (p<0.05 were detected in performance, carcass weight, weight and breast yield, and leg weight. The best results were obtained with 33 and 66 g/kg as compared to the control diet and 100 g SSM /kg. Abdominal fat decreased with the inclusion of 66 and 100 g SSM / kg, but the sensory quality of breast and thighs was not affected by the inclusion of SSM. The serum levels of total cholesterol, very low density (VLDL and low density (LDL lipoproteins, triglycerides, glucose and atherogenic index decreased with the inclusion of 100 g/kg of SSM, except for high density lipoproteins (HDL, which increased. The inclusion of 0, 33, 66 and 100 g/kg of SSM in broiler diets, partially replacing soybean meal and vegetable oil, improved live performance and edible portions yield. In addition, abdominal fat and serum levels of harmful lipids were reduced, whereas serum levels of beneficial lipids increased. There was no effect on meat sensory quality.

  15. Location of Caspase 3-like Protease in the Development of Sieve Element and Tracheary Element of Stem in Cucurbita moschata

    Institute of Scientific and Technical Information of China (English)

    Xia Hao; Jie Qian; Shan Xu; Xin Song; Jian Zhu

    2008-01-01

    The casepase is considered to regulate the process of programmed cell death in the development of organisms. In this study, caspase 3-like protease was detected by immunohistochemistry and immunoelectron microscopy during the development of sieve element and tracheary element of stem in Cucurbita moschata Duch. Antibody with brown color (under light microscopy) and gold particles (under transmission electron microscopy) for detecting caspase 3-like protease was mainly displayed in inner phloem, extemal phloem and xylem in the region close to procambium. From the results it was considered that caspase 3-like protease did exist in vascular elements and played different roles during the development of sieve and tracheary elements, and different types of programmed cell death might be carried out. The caspase 3-like protease mainly participated in making cytoplasmic streaming cease and in degrading P-protein bodies; however, it rarely participated in the function for signal transferring in the developmental sieve element. However, it might induce calcium accumulation for rupturing the tonoplast in the signal of PCD in the developmental tracheary element.

  16. Atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of Cucurbita moschata.

    Science.gov (United States)

    Hou, Xiaomin; Meehan, Edward J; Xie, Jieming; Huang, Mingdong; Chen, Minghuang; Chen, Liqing

    2008-10-01

    A novel type 1 ribosome-inactivating protein (RIP) designated cucurmosin was isolated from the sarcocarp of Cucurbita moschata (pumpkin). Besides rRNA N-glycosidase activity, cucurmosin exhibits strong cytotoxicities to three cancer cell lines of both human and murine origins, but low toxicity to normal cells. Plant genomic DNA extracted from the tender leaves was amplified by PCR between primers based on the N-terminal sequence and X-ray sequence of the C-terminal. The complete mature protein sequence was obtained from N-terminal protein sequencing and partial DNA sequencing, confirmed by high resolution crystal structure analysis. The crystal structure of cucurmosin has been determined at 1.04A, a resolution that has never been achieved before for any RIP. The structure contains two domains: a large N-terminal domain composed of seven alpha-helices and eight beta-strands, and a smaller C-terminal domain consisting of three alpha-helices and two beta-strands. The high resolution structure established a glycosylation pattern of GlcNAc(2)Man(3)Xyl. Asn225 was identified as a glycosylation site. Residues Tyr70, Tyr109, Glu158 and Arg161 define the active site of cucurmosin as an RNA N-glycosidase. The structural basis of cytotoxicity difference between cucurmosin and trichosanthin is discussed.

  17. Location of caspase 3-like protease in the development of sieve element and tracheary element of stem in Cucurbita moschata.

    Science.gov (United States)

    Hao, Xia; Qian, Jie; Xu, Shan; Song, Xin; Zhu, Jian

    2008-12-01

    The casepase is considered to regulate the process of programmed cell death in the development of organisms. In this study, caspase 3-like protease was detected by immunohistochemistry and immunoelectron microscopy during the development of sieve element and tracheary element of stem in Cucurbita moschata Duch. Antibody with brown color (under light microscopy) and gold particles (under transmission electron microscopy) for detecting caspase 3-like protease was mainly displayed in inner phloem, external phloem and xylem in the region close to procambium. From the results it was considered that caspase 3-like protease did exist in vascular elements and played different roles during the development of sieve and tracheary elements, and different types of programmed cell death might be carried out. The caspase 3-like protease mainly participated in making cytoplasmic streaming cease and in degrading P-protein bodies; however, it rarely participated in the function for signal transferring in the developmental sieve element. However, it might induce calcium accumulation for rupturing the tonoplast in the signal of PCD in the developmental tracheary element.

  18. [Effects of Celosia argentea and Cucurbita moschata extracts on anti-DNP IgE antibody production in mice].

    Science.gov (United States)

    Imaoka, K; Ushijima, H; Inouye, S; Takahashi, T; Kojima, Y

    1994-05-01

    We have already reported that the Perilla frutescens extract (PFE) suppressed anti-DNA IgE antibody production in mice. In this study, we prepared extracts of Celosia argentea L. (CAE) and Cucurbita moschata Duch (CME), which are Chinese herbal medicines like Perilla frutescens, and examined the effects on anti-DNP antibody responses in mice. To examine the effects of CAE & CME on primary antibody responses, CAE & CME were intraperitoneally injected the day before primary immunization of DNP-ovalbumin. Anti-DNP antibody production was markedly suppressed. Then, we examined the effects on secondary antibody responses. CEA & CME were injected only the day before secondary immunization. Anti-DNP IgE production was markedly suppressed, but IgG responses were not affected. It was also found that mitogenic activity occurred in CAE & CME dose dependently in vitro. These effects of CAE & CME were superior to that of PFE. These results suggest that CAE & CME may be more useful than PFE for the suppression of IgE antibody in certain allergic disorders.

  19. Atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of Cucurbita moschata

    Energy Technology Data Exchange (ETDEWEB)

    Hou, Xiaomin; Meehan, Edward J.; Xie, Jieming; Huang, Mingdong; Chen, Minghuang; Chen, Liqing (UAH); (Fujian); (Chinese Aca. Sci.)

    2008-10-27

    A novel type 1 ribosome-inactivating protein (RIP) designated cucurmosin was isolated from the sarcocarp of Cucurbita moschata (pumpkin). Besides rRNA N-glycosidase activity, cucurmosin exhibits strong cytotoxicities to three cancer cell lines of both human and murine origins, but low toxicity to normal cells. Plant genomic DNA extracted from the tender leaves was amplified by PCR between primers based on the N-terminal sequence and X-ray sequence of the C-terminal. The complete mature protein sequence was obtained from N-terminal protein sequencing and partial DNA sequencing, confirmed by high resolution crystal structure analysis. The crystal structure of cucurmosin has been determined at 1.04 {angstrom}, a resolution that has never been achieved before for any RIP. The structure contains two domains: a large N-terminal domain composed of seven {alpha}-helices and eight {beta}-strands, and a smaller C-terminal domain consisting of three {alpha}-helices and two {beta}-strands. The high resolution structure established a glycosylation pattern of GlcNAc{sub 2}Man3Xyl. Asn225 was identified as a glycosylation site. Residues Tyr70, Tyr109, Glu158 and Arg161 define the active site of cucurmosin as an RNA N-glycosidase. The structural basis of cytotoxicity difference between cucurmosin and trichosanthin is discussed.

  20. Subcellular distribution and uptake mechanism of di-n-butyl phthalate in roots of pumpkin (Cucurbita moschata) seedlings.

    Science.gov (United States)

    Lin, Qingqi; Yang, Xiuhong; Huang, Xiongfei; Wang, Shizhong; Chao, Yuanqing; Qiu, Rongliang

    2016-01-01

    Phthalate acid esters (PAEs) are of particular concern due to their potential environmental risk to human and nonhuman organisms. Although uptake of PAEs by plants has been reported by several researchers, information about the intracellular distribution and uptake mechanisms of PAEs is still lacking. In this study, a series of hydroponic experiments using intact pumpkin (Cucurbita moschata) seedlings was conducted to investigate how di-n-butyl phthalate (DnBP), one of the most frequently identified PAEs in the environment, enters and is distributed in roots. DnBP was transported into subcellular tissues rapidly in the initial uptake period (<12 h). More than 80% of DnBP was detected in the cell walls and organelles, which suggests that DnBP is primarily accumulated in these two fractions due to their high affinity to DnBP. The kinetics of DnBP uptake were fitted well with the Michaelis-Menten equation, suggesting that a carrier-mediated process was involved. The application of 2,4-dinitrophenol and sodium vanadate reduced the uptake of DnBP by 37 and 26%, respectively, while aquaporin inhibitors, silver and glycerol, had no effect on DnBP uptake. These data demonstrated that the uptake of DnBP included a carrier-mediated and energy-dependent process without the participation of aquaporins.

  1. EVALUACIÓN DE PARÁMETROS PRODUCTIVOS Y PIGMENTACIÓN EN POLLOS ALIMENTADOS CON HARINA ZAPALLO (Cucurbita moschata

    Directory of Open Access Journals (Sweden)

    JULIANA CARVAJAL TAPIA

    2017-12-01

    Full Text Available On the farms you can get different products from small farming system that can contribute to the economy of the poultyman farmer, for example, the squash (Cucurbita moschata. With the squash flour was evaluated pigmentation of skin and productive parameters in broilers. With the inclusion of 0, 7,5 and 15 percent in diets. A design completely at random (DCA was used with four repetitions and four animals per experimental unit. The analysis of variety did not show statistic differences (p≤0,05 for the feed intake in the two phases, either in weight gain and feed conversion in the ending phase; there were showed meaningful statistic differences for weight gain and feed conversion in the starter phase and the pigmentation in the final phase, the skin pigmentation of animals fed with squash flour was intense yellow according to the variety of colors DMS compared to those fed with the witness treatment. The inclusion of squash flour in diets for feeding broiler chicken bigger than 7,5% y less than 15%, is considered as a no conventional raw material that helps to the pigmentation of the skin, without affecting productive parameters in the ending phase and it becomes in an alternative for the productive systems of the small producers generating a value added in terms of skin pigmentation.

  2. 'Brasileirinha': cultivar de abóbora (Cucurbita moschata de frutos bicolores com valor ornamental e aptidão para consumo verde 'Brasileirinha': an ornamental bicolor squash (Cucurbita moschata cultivar for immature fruit consumption

    Directory of Open Access Journals (Sweden)

    Leonardo S Boiteux

    2007-03-01

    Full Text Available 'Brasileirinha' é uma cultivar de abóbora (Cucurbita moschata com frutos bicolores que foi desenvolvida com o objetivo de disponibilizar um produto diferenciado devido ao aspecto ornamental e a composição nutricional de seus frutos. Esta cultivar foi selecionada na geração F7, sendo obtida via cruzamentos convencionais entre um acesso de frutos bicolores, provavelmente devido à presença de um alelo do gene B, e a cultivar Mocinha (com frutos imaturos de cor verde uniforme. A característica peculiar da cultivar Brasileirinha é a produção de frutos com casca apresentando uma marcante coloração bicolor (coloração amarela na região proximal e verde na posição distal dos frutos. A polpa apresenta coloração amarela-esverdeada em frutos colhidos imaturos e, à medida que o fruto amadurece, intensifica-se uma coloração alaranjada. Beta-caroteno e luteína são os principais carotenóides presentes em frutos para consumo verde. Em frutos em completo estádio de maturação (polpa laranja intensa verifica-se a acumulação de beta-caroteno e alfa-caroteno (precursores da vitamina A em torno de 243 mg g-1. A cultivar Brasileirinha tem apresentado boa resistência de campo a diferentes raças de oídio (Podosphaera xanthii. Esta cultivar é preferencialmente recomendada para consumo como abobrinha verde (no estádio de fruto imaturo e para fins ornamentais (frutos em todos os estádios. Uma opção é o uso de frutos jovens em conservas. A cultivar Brasileirinha é recomendada para plantio em todas as tradicionais regiões produtoras do país. O sistema de produção para esta cultivar tem sido o mesmo adotado para outros tipos de abóboras.'Brasileirinha' is a squash (Cucurbita moschata cultivar developed by Embrapa Vegetable Crops, with a appealing ornamental appearance and carotenoid composition of its fruits might provide raw material for the development of value-added products targeting new market niches. This cultivar is an F7

  3. Does crotalaria (Crotalaria breviflora or pumpkin (Cucurbita moschata inter-row cultivation in restoration plantings control invasive grasses?

    Directory of Open Access Journals (Sweden)

    Ricardo Gomes César

    2013-08-01

    Full Text Available Alternative methods to control invasive fodder grasses are necessary to reduce the use of herbicides in forest restoration, which has been carried out primarily in riparian zones. We sought to investigate if inter-row cultivation of crotalaria (Crotalaria breviflora DC or pumpkin (Cucurbita moschata Duschene ex. Poir with native tree species is an efficient strategy to control invasive fodder grasses in restoration plantings. We tested five treatments in a randomized block design, namely (1 control of brachiaria grass (Urochloa decumbens (Stapf. Webster with glyphosate in the implementation and post-planting grass control of the reforestation, (2 and 3 glyphosate use in the implementation and inter-row sowing of crotalaria (2 or pumpkin (3, and control of brachiaria by mowing in the post-planting phase, (4 and 5 mowing in the implementation and inter-row sowing of crotalaria (4 or pumpkin (5, and control of brachiaria by mowing in the post-planting phase. Post-planting grass control was carried out four and nine months after tree seedling planting. Throughout 13 months, we evaluated the percentage of ground cover by brachiaria grass, pumpkin production, and native tree seedling mortality, height and crown cover. The exclusive use of glyphosate, without inter-row sowing of pumpkin or crotalaria showed the most favorable results for controlling brachiaria grass and, consequently, for tree seedling development. Hence, inter-row cultivation of green manure or short-lived crop species is not enough to control invasive grasses in restoration plantings, and complementary weeding is necessary to reduce the highly competitive potential of C4 grasses for supporting native species seedlings growth.

  4. Dynamics of Selected Bioactive Substances Changes in Cucurbita Moschata Duch. Ex Poir. After Storage and Different Methods of Technological Processing

    Directory of Open Access Journals (Sweden)

    Alena Andrejiová

    2016-01-01

    Full Text Available The winter squash is an important source of antioxidants, especially carotenoids. The aim of submitted research work was to determine the effect of genotype, storage and different methods of technological processing (baking, boiling and sterilization on the content of ascorbic acid and total carotenoids in fruits of winter squash (Cucurbita moschata Duch. ex Poir.. The small-plot field experiment was established at Slovak University of Agriculture in Nitra in 2013. Five cultivars of winter squash (‘Liscia’, ‘Orange’, ‘Hannah’, ‘UG 205 F1’ and ‘Waltham’ were examined in experiment. The total carotenoids content in the pulp of fresh fruits was ranged from 9.33 to 15.10 mg.100 g−1. Its highest value was determined in case of ‘Orange’ variety. The storage and the thermal treatment of fruit pulp in case of baking had positive impact from the total carotenoid content point of view. The baking resulted in the increase of its value in winter squash. On the contrary, sterilization tended to the decrease of total carotenoid content in edible part of squash. The total carotenoids content in the baking pulp was ranged from 14.27 to 31.87 mg.100 g−1. The vitamin C content before storage and technological processing ranged in interval from 13.88 to 18.69 mg.100 g−1. Particular thermal methods of processing and storage resulted in decrease of vitamin C content in the pulp of all winter squash varieties.

  5. Caracterización molecular de introducciones colombianas de zapallo Cucurbita moschata

    Directory of Open Access Journals (Sweden)

    Javier A. Restrepo S

    2008-01-01

    Full Text Available Se realizó la caracterización molecular mediante el polimorfismo en la longitud de fragmentos amplificados (AFLP de 121 introducciones de C. moschata, del Banco de Germoplasma del Programa de Investigación en Hortalizas de la Universidad Nacional de Colombia Sede Palmira, provenientes de ocho departamentos de Colombia. Los datos AFLP se evaluaron utilizando análisis de correspondencia múltiple (ACM, distancia genética, análisis genético con el programa TFPGA y el método de agrupamiento UPGMA. La diversidad genética de estas introducciones fue alta y estuvo de acuerdo con la diversidad morfoagronómica estudiada previamente. Los valores Fst indicaron que existe estructura genética entre la mayoría de las introducciones. La mayoría de la variación genética entre las introducciones se atribuyó a variación entre individuos dentro de cada departamento.

  6. 中国南瓜无蔓性状种间遗传研究%Genetic Study on the Vineless Characters of Cucurbita moschata among Species

    Institute of Scientific and Technical Information of China (English)

    马海龙; 智海英; 岳青; 梁燕平; 苗如意

    2011-01-01

    In order to inquire the meaning and feasibling on transfer the vineless character from C. moschata into C. maxima and C. pepo, the interspecific crossing between C. moschata materials with vineless character and C. maxima materials or C. pepo materials was conducted, we studied the interspecific genetic development on vineless character by the RAPD molecular marker and field investigation. Genetic performance of the vineless character of Cucurbita moschata in three mainly cultivated species in Cucurbita was studied in this paper. The results showed that the segregation ratios of the vineless character in F1 group, F2 group and BC1F1 group of the interspecific hybrids fit the Mendelian law. It was different from intraspecific hybridization that the first filial generation between vineless C. moschata and the other vine pumpkin species showed that the internode elongated and the inheritance belonged to incomplete dominant inheritance. The unique vineless character of C. moschata was transferred to C. pepo and C. maxima by the means of interspecific hybridization and the brand-new germplasm resources were created.%为探讨向印度南瓜及美洲南瓜中转育中国南瓜无蔓性状的意义及可行性,进行了无蔓中国南瓜种间杂交试验,采用田间调查结合RAPD分子标记鉴定的方法研究中国南瓜无蔓性状种间遗传规律.通过比较中国南瓜无蔓性状在南瓜属3个种中的遗传表现发现:中国南瓜无蔓性状在种间杂种F1、F2及BC1F1中的分离比例符合孟德尔遗传规律;与种内杂交不同,无蔓中国南瓜与蔓生异种南瓜材料的种间杂交一代表现出节间变长的倾向,表现为不完全显性遗传;通过种间杂交,可将中国南瓜特有的无蔓性状转育到美洲南瓜及印度南瓜中,创造全新的种质资源.

  7. Morphological, biochemical and molecular characterization of Herpetomonas samuelpessoai camargoi n. subsp., a trypanosomatid isolated from the flower of the squash Cucurbita moschata.

    Science.gov (United States)

    Fiorini, J E; Takata, C S; Teofilo, V M; Nascimento, L C; Faria-e-Silva, P M; Soares, M J; Teixeira, M M; De Souza, W

    2001-01-01

    We report the morphological, biochemical and molecular characteristics of a trypanosomatid isolated from the flower of Cucurbita moschata. Although the trypanosomatid was isolated from a plant, the lack of recognition of Phytomonas-specific molecular markers based on spliced-leader and ribosomal genes as well as by monoclonal antibodies specific for Phytomonas argues against assigning it to this genus. Because the isolate displayed typical opisthomastigote forms in culture, it is assigned to the genus Herpetomonas. Analysis of randomly amplified polymorphic DNA (RAPD) patterns and characterization of ribosomal SSU and ITS markers suggest that it is more closely related to H. samuelpessoai than to any other species. However, the presence of spined flagellates in culture (displaying lateral expansions of the plasma membrane originating near the flagellar pocket) and isolate-specific RAPD fingerprints argue strongly that the trypanosomatid belongs to a new subspecies, for which the name Herpetomonas samuelpessoai camargoi n. subsp. is proposed.

  8. 中国南瓜幼苗植物学数量性状的评价%Evaluation on Botanical Quantitative Characters of Cucurbita moschata Seedling

    Institute of Scientific and Technical Information of China (English)

    周俊国; 扈惠灵; 李新峥; 李海真

    2010-01-01

    以135份中国南瓜(Cucurbita moschata Duch.)农家品种资源的自交系为试验材料,调查分析了中国南瓜自交系幼苗的下胚轴粗度、下胚轴高度、子叶长度、子叶宽度和予叶形状等5个数量性状,并对这些数量性状进行了统计分析,提出了描述评价标准,为研制中国南瓜特异性一一致性-稳定性(Distinetness-Uniformity-Stability,DUS)测试标准中幼苗性状的测试内容奠定了基础.

  9. 南瓜主要病毒病的细胞病理观察%Cytopathologic observation on main viruses causing disease of pumpkin(Cucurbita moschata)

    Institute of Scientific and Technical Information of China (English)

    方琦; 杨国慧; 丁铭; 李婷婷; 张丽珍; 彭潞波; 苏晓霞; 张仲凯

    2004-01-01

    南瓜属葫芦科南瓜属(Cucurbita moschata),一年生草本植物,是常见的蔬菜品种。由于南瓜的经济价值和药用价值高,近年来南瓜产业得到了迅速发展,随着南瓜种植面积不断扩大,随之而来的南瓜病毒病也日益加剧,致使南瓜的产量和品质受到严重影响。为了解决南瓜病毒病给生产带来的危害,在

  10. Ribosome-inactivating proteins in edible plants and purification and characterization of a new ribosome-inactivating protein from Cucurbita moschata.

    Science.gov (United States)

    Barbieri, Luigi; Polito, Letizia; Bolognesi, Andrea; Ciani, Marialibera; Pelosi, Emanuele; Farini, Valentina; Jha, Ajay K; Sharma, Neelam; Vivanco, Jorge M; Chambery, Angela; Parente, Augusto; Stirpe, Fiorenzo

    2006-05-01

    The basic protein fraction of tissue extracts from 40 edible plants inhibited cell-free protein synthesis and released adenine from herring sperm DNA, thus having adenine glycosylase activity. This suggested the presence of ribosome-inactivating proteins (RIPs) in the plant extracts. This indication was further strengthened by the presence of the two activities after a partial chromatographic purification of three extracts, including that from Lycopersicon esculentum (tomato), which had very low activity. From the extract of Cucurbita moschata (pumpkin), the most active one, a glycoprotein of 30,665 Da was purified which had the properties of a RIP, in that (i) it inhibited protein synthesis by a rabbit reticulocyte lysate with IC50 (concentration giving 50% inhibition) 0.035 nM (1.08 ng ml(-1)) and by HeLa, HT29 and JM cells with IC50 in the 100 nM range, (ii) deadenylated hsDNA and other polynucleotidic substrates, and (iii) depurinated yeast rRNA at a concentration of 0.1 ng ml(-1), all values being comparable to those of other RIPs. The C. moschata RIP gave a weak cross-reaction only with an antiserum against dianthin 32, but not with antisera against other RIPs, and had superoxide dismutase, antifungal and antibacterial activities.

  11. Evaluación de familias de zapallo (Cucurbita moschata Duch. seleccionadas por mayor contenido de materia seca en el fruto y otras características agronómicas Evaluation of squash (Cucurbita moschata Duch. families selected for higher content of dry matter in the fruit and other agronomic traits

    Directory of Open Access Journals (Sweden)

    Dora Enith Tobar Tosse

    2010-01-01

    Full Text Available Se evaluaron cuatro familias de hermanos completos de zapallo, Cucurbita moschata Duch, provenientes de dos ciclos de estabilización genética mediante cruzamientos fraternales y seleccionadas por mayor contenido de materia seca (MS en el fruto y buenas características para consumo en fresco. La familia F7a sobresalió por su mayor contenido de materia MS en el fruto (15.86% ± 2.17 en comparación con el testigo comercial Unapal-Bolo Verde (10.68% ± 2.08, forma esférica levemente achatada, color externo amarillo claro, color de pulpa amarilla a salmón, espesor de pulpa de 3.71 ± 0.58 cm y peso promedio del fruto de 3.10 ± 0.73 kg, que la convierte en una alternativa para consumo en fresco.Four full-sib families of squash, Cucurbita moschata Duch, from two cycles of genetic stability through crosses fraternal and selected by a higher content of dry matter in fruit and good characteristics for fresh consumption was evaluated. The F7a family had at increased dry matter content in fruit (15.86% ± 2.17 compared with the control-commercial Unapal Bolo Verde (10.68% ± 2.08, slightly flattened spherical form, external color yellow, light yellow flesh color to salmon, flesh thickness of 3.71 cm ± 0.58 and average fruit weight of 3.10 kg ± 0.73, which makes it an alternative for fresh consumption.

  12. A water-soluble extract from Cucurbita moschata shows anti-obesity effects by controlling lipid metabolism in a high fat diet-induced obesity mouse model.

    Science.gov (United States)

    Choi, Hyounjeong; Eo, Haekwan; Park, Kyoungcheol; Jin, Mirim; Park, Eun-Jin; Kim, Seon-Hee; Park, Jeong Euy; Kim, Sunyoung

    2007-08-01

    During the screening of a variety of plant sources for their anti-obesity activity, it was found that a water-soluble extract, named PG105, prepared from stem parts of Cucurbita moschata, contains potent anti-obesity activities in a high fat diet-induced obesity mouse model. In this animal model, increases in body weight and fat storage were suppressed by 8-week oral administration of PG105 at 500 mg/kg, while the overall amount of food intake was not affected. Furthermore, PG105 protected the development of fatty liver and increased the hepatic beta-oxidation activity. Results from blood analysis showed that the levels of triglyceride and cholesterol were significantly lowered by PG105 administration, and also that the level of leptin was reduced, while that of adiponectin was increased. To understand the underlying mechanism at the molecular level, the effects of PG105 were examined on the expression of the genes involved in lipid metabolism by Northern blot analysis. In the liver of PG105-treated mice, the mRNA level of lipogenic genes such as SREBP-1c and SCD-1 was decreased, while that of lipolytic genes such as PPARalpha, ACO-1, CPT-1, and UCP-2 was modestly increased. Our data suggest that PG105 may have great potential as a novel anti-obesity agent in that both inhibition of lipid synthesis and acceleration of fatty acid breakdown are induced by this reagent.

  13. Establishment of Cucurbita moschata Genetic Transformation System by Agrobacterium tumefaciens Transfection%农杆菌介导南瓜遗传转化体系的建立

    Institute of Scientific and Technical Information of China (English)

    付洪冰; 崔崇士; 赵曦; 刘琦

    2010-01-01

    以南瓜金辉一号(Cucurbita moschata 'Jinhui 1')为实验材料,利用根癌农杆菌(Agrobacterium tumefaciens)介导转化南瓜子叶节,研究了预培养时间、侵染时间、乙酰丁香酮(AS)浓度和共培养时间,抗生素羧苄青霉素(Carb)、头孢霉素(Cef)以及筛选剂卡那霉素(Kan)等因素对离体不定芽的影响,建立了南瓜最适遗传转化体系.结果表明:外植体预培养0天,侵染时间30分钟,AS浓度为100 mg·L-1,共培养5天可获得最高遗传转化效率;最适除菌剂为Cef,其最适浓度为500mg·L-1;最适Kan筛选浓度为100 mg·L-1:在MS培养基上培养抗性芽生根,经PCR和Southern blot检测,证明为转基因植株.

  14. Effect of drying and co-matrix addition on the yield and quality of supercritical CO₂ extracted pumpkin (Cucurbita moschata Duch.) oil.

    Science.gov (United States)

    Durante, Miriana; Lenucci, Marcello S; D'Amico, Leone; Piro, Gabriella; Mita, Giovanni

    2014-04-01

    In this work a process for obtaining high vitamin E and carotenoid yields by supercritical carbon dioxide (SC-CO₂) extraction from pumpkin (Cucurbita moschata Duch.) is described. The results show that the use of a vacuum oven-dried [residual moisture (∼8%)] and milled (70 mesh sieve) pumpkin flesh matrix increased SC-CO₂ extraction yields of total vitamin E and carotenoids of ∼12.0- and ∼8.5-fold, respectively, with respect to the use of a freeze-dried and milled flesh matrix. The addition of milled (35 mesh) pumpkin seeds as co-matrix (1:1, w/w) allowed a further ∼1.6-fold increase in carotenoid yield, besides to a valuable enrichment of the extracted oil in vitamin E (274 mg/100 g oil) and polyunsaturated fatty acids. These findings encourage further studies in order to scale up the process for possible industrial production of high quality bioactive ingredients from pumpkin useful in functional food or cosmeceutical formulation.

  15. Effect of gamma irradiation and storage time on microbial growth and physicochemical characteristics of pumpkin (Cucurbita Moschata Duchesne ex Poiret) puree.

    Science.gov (United States)

    Gliemmo, María F; Latorre, María E; Narvaiz, Patricia; Campos, Carmen A; Gerschenson, Lía N

    2014-01-01

    The effect of gamma irradiation (0-2 kGy) and storage time (0-28 days) on microbial growth and physicochemical characteristics of a packed pumpkin puree was studied. For that purpose, a factorial design was applied. The puree contained potassium sorbate, glucose and vanillin was stored at 25°C . Gamma irradiation diminished and storage time increased microbial growth. A synergistic effect between both variables on microbial growth was observed. Storage time decreased pH and color of purees. Sorbate content decreased with storage time and gamma irradiation. Mathematical models of microbial growth generated by the factorial design allowed estimating that a puree absorbing 1.63 kGy would have a shelf-life of 4 days. In order to improve this time, some changes in the applied hurdles were assayed. These included a thermal treatment before irradiation, a reduction of irradiation dose to 0.75 kGy and a decrease in storage temperature at 20°C . As a result, the shelf-life of purees increased to 28 days.

  16. Influence of Nitrogen Sources and Plant Growth-Promoting Rhizobacteria Inoculation on Growth, Crude Fiber and Nutrient Uptake in Squash (Cucurbita moschata Duchesne ex Poir.) Plants

    OpenAIRE

    Alice I. TCHIAZE; Victor D. TAFFOUO; Henri FANKEM; Kenne, Martin; Régis BAZIRAMAKENGA; Ekodeck, Georges E.; Antoun, Hani

    2016-01-01

    Plant growth promoting rhizobacteria (PGPR, B) have immense potential application in sustainable agriculture as ecofriendly biofertilizers and biopesticides. In this study, the effects of three nitrogen (N) sources (NO3-, NH4+ and NO3NH4) and PGPR on growth, crude fiber and nutrient uptake were investigated in squash plants. Some growth parameters [root dry weight (RDW), shoot dry weight (SDW), total plant dry weight (PDW), number of leaves (NL), shoot length (SL), stem diameter (SD) and numb...

  17. Influence of Nitrogen Sources and Plant Growth-Promoting Rhizobacteria Inoculation on Growth, Crude Fiber and Nutrient Uptake in Squash (Cucurbita moschata Duchesne ex Poir. Plants

    Directory of Open Access Journals (Sweden)

    Alice I. TCHIAZE

    2016-06-01

    Full Text Available Plant growth promoting rhizobacteria (PGPR, B have immense potential application in sustainable agriculture as ecofriendly biofertilizers and biopesticides. In this study, the effects of three nitrogen (N sources (NO3-, NH4+ and NO3NH4 and PGPR on growth, crude fiber and nutrient uptake were investigated in squash plants. Some growth parameters [root dry weight (RDW, shoot dry weight (SDW, total plant dry weight (PDW, number of leaves (NL, shoot length (SL, stem diameter (SD and number of ramifications (NR], crude fiber (cellulose content and nutrient uptake (N, P, K, Ca, Mg, Na, Fe, Cu, Mn and Zn were determined. Application of NO3-,NH4+ or NO3NH4 singly or in combination with PGPR inoculation led to a significant increase in RDW, SDW, PDW, NL, SL, SD and NR. Na, Cu and Zn contents, on the contrary, decreased in inoculated treated plants while no significant differences were recorded in cellulose contents (CE of leaves except in plants fed with NO3-. The leaf CE content ranged from 12.58 to 13.67%. The plants supplied with NO3+B, NH4+B and NO3NH4+B showed significantly higher plant biomass and accumulation of N, P, K and Mn concentrations in leaves compared to all other treatments. These results suggest that specific combinations of PGPR with NO3-, NH4+ or NO3NH4 fertilizers can be considered as efficient alternative biofertilizers to improve significantly the squash growth and nutrient uptake.

  18. Herança de formato e produção de frutos imaturos em abobrinha (Cucurbita moschata Inheritance of immature fruit shape and production in summer squash (Cucurbita moschata

    Directory of Open Access Journals (Sweden)

    A.I.I. Cardoso

    1995-12-01

    Full Text Available O objetivo deste trabalho foi determinar a herança de formato e produção de frutos imaturos de 2 novas linhagens de C. moschata. Para tal obtiveram-se as gerações Fl, F2 e retrocruzamentos para ambos os progenitores. Para a avaliação das diferentes gerações utilizou-se o delineamento em blocos ao acaso com 4 repetições e número variável de plantas por repetição. As estimativas dos parâmetros genéticos foram obtidas segundo o modelo de MATHER & JINKS. Foram avaliados o peso, comprimento e diâmetros próximos das extremidades peduncular e estilar dos frutos. Observou-se dominância para maior velocidade de crescimento dos frutos, caracterizada pelos maiores comprimentos, diâmetros e peso médio na geração F1. Observou-se também dominancia para frutos mais bojudos e com menor relação entre o comprimento e a média dos diâmetros. Devido ao maior peso médio de frutos, e não ao número, obteve-se heterose significativa (20,5% para produção total por planta.Two inbred lines of C. moschata were crossed to obtain Fl, F2 and F1BC1 for both parental generations to study the inheritance of immature fruit shape and production. Four replications for each generation were choosen in a randomized block design. For each one of the eleven harvestings, the following parameters were evaluated: weight, length and diameter near peduncle and style ends of the immature fruits. Through the results it is concluded that there is dominance for higher growth speed of the fruits, which determines greater length, diameters and weight, when they were harvested at the same age, and, therefore, greater yield per plant.

  19. Characterization of acid-extracted pectin-enriched products obtained from red beet (Beta vulgaris L. var. conditiva) and butternut (Cucurbita moschata Duch ex Poiret).

    Science.gov (United States)

    Fissore, Eliana N; Ponce, Nora M A; de Escalada Pla, Marina; Stortz, Carlos A; Rojas, Ana M; Gerschenson, Lía N

    2010-03-24

    Chemical and rheological characteristics of fractions enriched in soluble dietary fiber are reported. These fractions were obtained through acid hydrolysis of butternut (Cucurbita moschata Duch ex Poiret) and red beet (Beta vulgaris L. var. conditiva) cell wall enriched powders. Hydrolysis was performed using citric acid at different pH values and reaction times (2 and 3 h). Yields obtained for butternut fractions were between 21 and 28 g/100 g; for red beet, yields were 24 and 31 g/100 g for pH 1.5 and 11 and 17 g/100 g for pH 2.0 for previously mentioned times; in general, the increase of the yield was directly correlated with the decrease of pH and the increase of reaction time. Products enriched in low methoxyl pectins were obtained in all cases. At the lowest pH assayed, pectins were essentially constituted by homogalacturonan; a significant content of neutral sugars was determined at the higher extraction pH. Neutral sugars were constituted mainly by arabinose, galactose, rhamnose, and glucose in different proportions for each fraction; in general, butternut fractions showed high glucose contents. Flow behavior for 2.00% (w/v) aqueous systems of the different products was evaluated. Data obtained for fractions isolated at pH 1.5 fit to Herschel-Bulkley and Cross models while those isolated at pH 2.0 fit to Ostwald and Cross models. All samples showed low viscosity and, hence, poor thickening properties.

  20. Dehydrodiconiferyl alcohol isolated from Cucurbita moschata shows anti-adipogenic and anti-lipogenic effects in 3T3-L1 cells and primary mouse embryonic fibroblasts.

    Science.gov (United States)

    Lee, Junghun; Kim, Donghyun; Choi, Jonghyun; Choi, Hyounjeong; Ryu, Jae-Ha; Jeong, Jinhyun; Park, Eun-Jin; Kim, Seon-Hee; Kim, Sunyoung

    2012-03-16

    A water-soluble extract from the stems of Cucurbita moschata, code named PG105, was previously found to contain strong anti-obesity activities in a high fat diet-induced obesity mouse model. One of its biological characteristics is that it inhibits 3T3-L1 adipocyte differentiation. To isolate the biologically active compound(s), conventional solvent fractionation was performed, and the various fractions were tested for anti-adipogenic activity using Oil Red O staining method. A single spot on thin layer chromatography of the chloroform fraction showed a potent anti-adipogenic activity. When purified, the structure of its major component was resolved as dehydrodiconiferyl alcohol (DHCA), a lignan, by NMR and mass spectrometry analysis. In 3T3-L1 cells, synthesized DHCA significantly reduced the expression of several adipocyte marker genes, including peroxisome proliferator-activated receptor γ (Pparg), CCAAT/enhancer-binding protein α (Cebpa), fatty acid-binding protein 4 (Fabp4), sterol response element-binding protein-1c (Srebp1c), and stearoyl-coenzyme A desaturase-1 (Scd), and decreased lipid accumulation without affecting cell viability. DHCA also suppressed the mitotic clonal expansion of preadipocytes (an early event of adipogenesis), probably by suppressing the DNA binding activity of C/EBPβ, and lowered the production level of cyclinA and cyclin-dependent kinase 2 (Cdk2), coinciding with the decrease in DNA synthesis and cell division. In addition, DHCA directly inhibited the expression of SREBP-1c and SCD-1. Similar observations were made, using primary mouse embryonic fibroblasts. Taken together, our data indicate that DHCA may contain dual activities, affecting both adipogenesis and lipogenesis.

  1. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata) Seeds and Recycling of Phase Components.

    Science.gov (United States)

    Amid, Mehrnoush; Manap, Mohd Yazid; Hussin, Muhaini; Mustafa, Shuhaimi

    2015-06-17

    Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w) Triton X-100 and 20% (w/w) xylitol, at 56.2% of tie line length (TLL), (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases) and a crude load of 25% (w/w) at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  2. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata Seeds and Recycling of Phase Components

    Directory of Open Access Journals (Sweden)

    Mehrnoush Amid

    2015-06-01

    Full Text Available Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w Triton X-100 and 20% (w/w xylitol, at 56.2% of tie line length (TLL, (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases and a crude load of 25% (w/w at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  3. EFEITOS DE PODA DE RAMA NA PRODUÇÃO DE ABOBRINHA (Curcubita moschata CV. “MENINA BRASILEIRA” INFLUENCE OF VINE PRUMING ON THE YIELD OF SQUASH (Cucurbita moschata CV. ‘MENINA BRASILEIRA’

    Directory of Open Access Journals (Sweden)

    João Batista Duarte

    2007-09-01

    Full Text Available

    Alguns produtores hortícolas goianos cortam a ponta da rama principal da aboboreira para estimular a ramificação lateral e aumentar e/ou antecipar a produção. Em experimento, realizado na área experimental da Escola de Agronomia da Universidade Federal de Goiás, Goiânia, avaliaram-se os efeitos deste tipo de poda na produção de abobrinha da cultivar ‘Menina Brasileira' . Em doze colheitas, feitas entre 17/11 e 23/12/93, determinou-se o número e o peso de frutos. O tratamento não influenciou significativamente a produção de frutos nem sua distribuição ao longo das colheitas. A produção das plantas podadas, porém, foi ligeiramente mais precoce, fato que exige maior investigação.

    PALAVRAS-CHAVE: Abobrinha; Cucurbita moschata; poda de rama.

    Some squash producers in the region of Goiânia (Brazil prune the top of the main vine in order to increase and/or anticipate production by stimulating lateral branching. In this trial, carried out at the Escola de Agronomia da Universidade Federal de Goiás, Goiânia, vine pruning was tested on the cv. ‘Menina Brasileira’. Fruits were harvested twice a week, from November 17 to December 23 (1993. Significant differences between treatment and control were found neither in number and weight of fruits nor in distribution of the yield over the harvest period. The pruned plants however were slightly earlier in production. This should be investigated with more accuracy.

    KEY-WORDS: Squash; vine pruning.

  4. Cloning and Sequence Analysis of CmNAC Gene from Cucurbita moschata%南瓜基因CmNAC的克隆与序列分析(英文)

    Institute of Scientific and Technical Information of China (English)

    郝芮; 赵福宽; 孙清鹏; 杨爱珍

    2012-01-01

    [Objective] The aim was to clone the CmNAC gene from Cucurbita moschata and analyze the sequence characteristics. [Method] A pair of degenerate primers was designed based on the conserved sequences of NAC gene from Brassica napus, Lycopersicon esculentum and Capsicum annuum. NAC transcription factor gene was amplified by RT-PCR from Cucurbita moschata leaves and cloned into pMD-19T vector; then the recombinant clones were sequenced. Finally, the sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software. [Result] The NAC transcription factor gene cloned from C. moschata included 442 bp encoding 147 amino acids, named CmNAC. The NAC gene fragment contained a conserved region like other plant NAC genes and belonged to the NAC family ATAF1/2 subfamily. [Conclusion] The stress resistance related gene NAC cloned from C. moschata is a foundation for further study on the biological function of the gene and plant genetic engineering.%[目的]克隆南瓜基因CmNAC并进行序列分析。[方法]以南瓜叶片为材料,根据甘蓝型油菜NAC1、番茄NAC和辣椒NAC保守结构域设计一对简并引物,采用RT-PCR方法扩增得出长度约为440bp大小的DNA片段,将其克隆至pMDl9-T载体上,对重组克隆进行测序,用BLAST和DNAMAN软件对核酸及氨基酸序列进行分析。[结果]所获得的南瓜NAC基因片段由442个碱基组成,编码147个氨基酸,命名为CmNAC;该基因片段具有其它植物NAC基因中存在的保守区,并且属于NAC家族中ATAF1/2亚家族。[结论]实验拟在南瓜中获得和抗逆性相关的NAC基因,为进一步研究该基因的生物学功能和植物基因工程奠定理论基础。

  5. Volatiles in Male Flowers of Cucurbita moschata by HS-SPME-GC-MS%HS-SPME-GC-MS分析金钩南瓜雄花挥发性成分

    Institute of Scientific and Technical Information of China (English)

    张伟; 卢引; 李昌勤; 康文艺

    2012-01-01

    目的:分析金钩南瓜雄花挥发性成分.方法:采用顶空固相微萃取和气质联用技术(HS-SPME-GC-MS)结合保留指数法,并用峰面积归一化法测定相对百分含量.结果:从金钩南瓜雄花中鉴定出59个化合物,占总峰面积的90.99%.结论:研究表明α-佛手柑油烯含量最高,占总挥发性成分的21.71%.该研究可为进一步开发利用南瓜资源提供科学依据.%Objective: To study the volatile constituents in male flower of Cucurbita moschata. Method; The volatiles were analyzed by head-space solid micro-extraction, coupled with GC-MS and Kovats indices. A quantitative analysis in percent was performed by peak area normalization measurements. Result; Fifty-nine compounds were identified in Jingou accounting for 90.99% of the total volatiles. Conclusion; a-bergamotene (21.71% ) was the highest. The study can provide scientific basis for the further development of the seeds of C. moschata.

  6. Volatile constituents from flower of Tianmian Cucurbita moschata Duch. by head-space solid micro-extraction coupled with GC-MS%HS-SPME-GC-MS分析甜面大南瓜花挥发性成分

    Institute of Scientific and Technical Information of China (English)

    李昌勤; 卢引; 李新铮; 邢晗; 康文艺

    2012-01-01

    Objective: To study the volatile constituents in flowers of Cucurbita moschata Duch. (Tianmian). Methods The volatiles were analyzed by head-space solid micro-extraction,coupled with GC-MS and Kovats indices for the first time. A quantitative analysis in percent was performed by peak area normalization measurements to search the volatile constituents from flowers of C. moschata Duch. (Tianmian). Results 37 compounds were identified from the male flowers and 16 from the female flowers,98.24% and 94.45% of the total essential constituents respectively,the male flowers and the female had 10 common components. Conclusion =The volatile constituents were different between the female flowers and the male of C. moschata.%目的:分析甜面大南瓜花挥发性成分。方法:采用顶空固相微萃取和气质联用技术(HS-SPME-GC-MS),结合保留指数法,首次采用峰面积归一化法计算各化合物的相对百分含量来分析甜面大南瓜花挥发性成分。结果:从甜面大南瓜雄花和雌花中分别鉴定出37和16种化合物,分别占总峰面积的98.24%和94.45%。其中雄花和雌花有10种共有成分。结论:发现甜面大南瓜雌雄花的挥发性成分具有差别。

  7. Cloning and Sequence Analysis of CmNA C Gene Fragments in Cucurbita moschata%南瓜CmNAC基因片段的克隆与序列分析

    Institute of Scientific and Technical Information of China (English)

    郝芮; 赵福宽; 孙清鹏; 杨爱珍

    2011-01-01

    NAC转录因子是特异存在于植物中具有多种生物功能的新型转录因子,其家族成员N端含有保守氨基酸序列,C端为高度变异的转录激活区.本研究以南瓜叶片为材料,根据甘蓝型油菜NAC1、番茄NAC、辣椒NAC保守结构域设计1对简并引物,采用RT-PCR方法扩增得出长度约为440 bp大小的DNA片段,将其克隆至pMDl9-T载体上,而后对重组克隆进行测序,用BLAST和DNAMAN软件对核酸及氨基酸序列进行分析,结果表明所获得的南瓜NAC基因片段由442个碱基组成,编码147个氨基酸,命名为CmNAG.该基因片段具有其他植物NAC基因中存在的保守区,并且属于NAC家族中ATAF1/2亚家族.%NAG transcription factors that are unique to plants are the new type transcription regulatory factors with multiple biological functions. Their family proteins contain a conserved amino acid sequence in N-terminal ends and highly different transcription regulatory domain in C terminal ends. In this experiment, a pair of degenerate primers was designed based on the conserved sequences of NAC gene from Brassica napus,tomato and Chili. NAC transcription factor gene fragment about 440 bp was amplified by RT-PCR from Cucurbita moschata leaves and cloned into pMD-19T vector, and then the recombinant clones were sequenced.The sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software. The results showed that NAC transcription factor gene gained from Cucurbita moschata included 442 bp, encoding 147 amino acids, which named CmNAC. The NAC gene fragments contained a conserved region like other plant NAC genes and belonged to the NAC family A TAF1/2 subfamily.

  8. Purification and characterization of Moschatin, a novel type I ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells.

    Science.gov (United States)

    Xia, Heng Chuan; Li, Feng; Li, Zhen; Zhang, Zu Chuan

    2003-10-01

    A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of approximately 29 kD. It is a rRNA N-glycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a IC50 of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M21 with a IC50 of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent.

  9. Purification and characterization of Moschatin, a novel type Ⅰ ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata),and preparation of its immunotoxin against human melanoma cells

    Institute of Scientific and Technical Information of China (English)

    CHAO TONG; HENG YU FAN; DA YUAN CHEN; XIANG FEN SONG; HEIDE SCHATTEN; QING YUAN SUN

    2003-01-01

    A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of~29 kD. It is a rRNA Nglycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a IC50 of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M21 with a IC50 of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent.

  10. Quantitative character analysis and cluster analysis of 37 pumpkin (Cucurbita moschata Duch) inbred- lines%南瓜自交系数量性状分析与聚类分析

    Institute of Scientific and Technical Information of China (English)

    周俊国; 李桂荣; 杨鹏鸣

    2006-01-01

    对南瓜[Cucurbita moschata(Duch.)Poiret]37个自交系的茎节间长、主蔓粗、叶片长、叶片宽、叶柄长、叶柄粗、主蔓第1朵雌花着生节位、主蔓20节内着生雌花数等8个数值数量性状的调查数据进行了统计分析.结果表明,所调查的数量性状变异系数有差异,主蔓20节内着生雌花数的变异系数达69.15%,最小的是叶柄长的变异系数,只有12.00%.主蔓第1雌花着生节位与主蔓20节内着生雌花数呈极显著的负相关,主蔓粗、叶柄粗均与主蔓第1雌花着生节位呈显著负相关,叶柄粗与主蔓20节内着生的雌花数呈显著正相关.37个南瓜自交系经聚类分析,可分为2个类群4个大类.

  11. Evaluating Criteria of Some Botanical Quantitative Characters of Vining Pumpkin (Cucurbita moschata Duch.) Germplasm Resources%蔓生型南瓜资源部分植物学数量性状的评价探讨

    Institute of Scientific and Technical Information of China (English)

    周俊国; 李新峥; 朱月林; 李海真

    2007-01-01

    对123份蔓生型南瓜(Cucurbita moschata Duch.)自交系的茎节间长度、主蔓粗、叶片长度、叶片宽度、叶柄长度、叶柄粗、第一朵雌花着生节位、主蔓20节内着生雌花数、花径9个数量性状进行了统计分析,提出节间长度、叶片长度、叶柄长度、第一雌花着生节位和主蔓20节内着生雌花数5个性状可以作为蔓生南瓜植物学性状评价的代表性状,并采用9级评价分级体系将这些代表性状进行评价分级,为建立规范化、标准化的南瓜种质资源评价系统奠定了基础.

  12. Sodium and chloride exclusion and retention by non-grafted and grafted melon and Cucurbita plants.

    Science.gov (United States)

    Edelstein, M; Plaut, Z; Ben-Hur, M

    2011-01-01

    The effects of grafting on Na and Cl(-) uptake and distribution in plant tissues were quantified in a greenhouse experiment using six combinations of melon (Cucumis melo L. cv. Arava) and pumpkin (Cucurbita maxima Duchesne×Cucurbita moschata Duchesne cv. TZ-148): non-grafted, self-grafted, melons grafted on pumpkins, and pumpkins grafted on melons. Total Na concentration in shoots of plants with pumpkin or melon rootstocks was 400 mmol kg(-1), respectively, regardless of the scion. In contrast, shoot Cl(-) concentrations were quite similar among the different scion-rootstock combinations. Na concentrations in exudates from cut stems of plants with a pumpkin rootstock were very low (<0.18 mM), whereas those in the exudates of plants with melon rootstocks ranged from 4.7 mM to 6.2 mM, and were quite similar to the Na concentration in the irrigation water. Root Na concentrations averaged 11.7 times those in the shoots of plants with pumpkin rootstocks, while in plants with melon rootstocks, values were similar. Two mechanisms could explain the decrease in shoot Na concentrations in plants with pumpkin rootstocks: (i) Na exclusion by the pumpkin roots; and (ii) Na retention and accumulation within the pumpkin rootstock. Quantitative analysis indicated that the pumpkin roots excluded ∼74% of available Na, while there was nearly no Na exclusion by melon roots. Na retention by the pumpkin rootstocks decreased its amount in the shoot by an average 46.9% compared with uniform Na distribution throughout the plant. In contrast, no retention of Na could be found in plants grafted on melons.

  13. 南瓜幼茎中螺纹导管次生壁的发育%Secondary Wall Formation of Helical Tracheary Elements in the Stem of Cucurbita Moschata

    Institute of Scientific and Technical Information of China (English)

    许珊; 祝建; 钱洁

    2006-01-01

    目的 观察南瓜(Cucurbita moschata)茎螺纹导管发育过程中次生壁的形成过程.方法 利用透射电镜对同一植株的不同茎段的螺纹导管次生壁及原生质体进行超微结构观察.结果 螺纹导管次生壁发育早期,细胞具有较大的细胞核,细胞质浓稠,具有核糖体、线粒体和高尔基体等细胞器.初生壁与质膜之间有间隔较规律的次生壁突起;中期,线粒体和高尔基体的数量明显增加,细胞质中几乎充满高尔基体及其囊泡,次生壁中微纤丝已成有序排列;晚期,细胞核消失,导管中央形成空腔,细胞器迅速减少,仅观察到少量高尔基体和线粒体,有大量周质微管沿细胞长轴分布,且在次生壁的周围有大量小泡附着,次生壁的分层结构清晰可见.结论 高尔基体及其分泌小泡和内质网等细胞器参与了导管次生壁的形成,其中周质微管也起了一定的作用.导管次生壁的形成与程序性细胞死亡(programmed cell death,PCD)密切相关.

  14. Effect of Chitosan Coating on the Quality of Fresh-cut Pumpkin(Cucurbita Moschata) during Storage%壳聚糖涂膜对鲜切南瓜贮藏品质的影响

    Institute of Scientific and Technical Information of China (English)

    张丹丹; 杨绍兰; 吴昊; 张宏斌; 王成荣

    2012-01-01

    Fresh "MiBen" pumpkins were subjected to coating with chitosan at different concentrations (0.5%, 1.0%, 1.5%)and subsequent storage at 4 ℃ in order to examine the fresh-keeping effect of chitosan coating on fresh-cut pumpkin. During the storage, changes in polysaccharose, carotenoid and total phenol contents, ethylene production, weight loss rate, firmness and the activity of PAL and POD of fresh-cut pumpkin were measured. Results showed that chitosan coating could delay the reductions of firmness, polysaccharose, carotenoid and total phenol contents, and also could delay the increase of ethylene production, obviously enhance PAL and POD activities, reduce weight loss rate, and substantially extend the shelf life of fresh-cut pumpkin, especially at a concentration of 1.0%.%以新鲜的“蜜本”南瓜(Cucurbita moschata)为试验材料,探讨不同质量分数(0.5%、1.0%、1.5%)壳聚糖涂膜液处理对鲜切南瓜贮藏品质的影响.结果表明:采用壳聚糖涂膜液处理,不同程度地延缓了鲜切南瓜乙烯释放量的增加和硬度的下降,阻止了多糖和类胡萝卜素的减少,抑制了POD、PAL酶活性的下降和总酚含量的降低.其中以质量分数为1.0%壳聚糖涂膜液处理的效果最好,有效地保持了鲜切南瓜贮藏期间的品质.

  15. Antioxidant enzymatic activities and gene expression associated with heat tolerance in the stems and roots of two cucurbit species ("Cucurbita maxima" and "Cucurbita moschata") and their interspecific inbred line "Maxchata".

    Science.gov (United States)

    Ara, Neelam; Nakkanong, Korakot; Lv, Wenhui; Yang, Jinghua; Hu, Zhongyuan; Zhang, Mingfang

    2013-12-10

    The elucidation of heat tolerance mechanisms is required to combat the challenges of global warming. This study aimed to determine the antioxidant enzyme responses to heat stress, at the enzymatic activity and gene expression levels, and to investigate the antioxidative alterations associated with heat tolerance in the stems and roots of squashes using three genotypes differing in heat tolerance. Plants of heat-tolerant "C. moschata", thermolabile "C. maxima" and moderately heat-tolerant interspecific inbred line "Maxchata" genotypes were exposed to moderate (37 °C) and severe (42 °C) heat shocks. "C. moschata" exhibited comparatively little oxidative damage, with the lowest hydrogen peroxide (H2O2), superoxide (O2(-)) and malondialdehyde (MDA) contents in the roots compared to stems, followed by "Maxchata". The enzyme activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) were found to be increased with heat stress in tolerant genotypes. The significant inductions of FeSOD, MnSOD, APX2, CAT1 and CAT3 isoforms in tolerant genotypes suggested their participation in heat tolerance. The differential isoform patterns of SOD, APX and CAT between stems and roots also indicated their tissue specificity. Furthermore, despite the sequence similarity of the studied antioxidant genes among "C. maxima" and "Maxchata", most of these genes were highly induced under heat stress in "Maxchata", which contributed to its heat tolerance. This phenomenon also indicated the involvement of other unknown genetic and/or epigenetic factors in controlling the expression of these antioxidant genes in squashes, which demands further exploration.

  16. Effect of disruption of a cutinase gene (cutA) on virulence and tissue specificity of Fusarium solani f. sp. cucurbitae race 2 toward Cucurbita maxima and C. moschata.

    Science.gov (United States)

    Crowhurst, R N; Binnie, S J; Bowen, J K; Hawthorne, B T; Plummer, K M; Rees-George, J; Rikkerink, E H; Templeton, M D

    1997-04-01

    A 3.9-kb genomic DNA fragment from the cucurbit pathogen Fusarium solani f. sp. cucurbitae race 2 was cloned. Sequence analysis revealed an open reading frame of 690 nucleotides interrupted by a single 51-bp intron. The nucleotide and predicted amino acid sequences showed 92 and 98% identity, respectively, to those of the cutA gene of the pea pathogen F. solani f. sp. pisi. A gene replacement vector was constructed and used to generate cutA- mutants that were detected with a polymerase chain reaction (PCR) assay. Seventy-one cutA- mutants were identified among the 416 transformants screened. Vector integration was assessed by Southern analysis in 23 of these mutants. PCR and Southern analysis data showed the level of homologous integration was 14%. Disruption of the cutA locus in mutants was confirmed by RNA gel blot hybridization. Neither virulence on Cucurbita maxima cv. Delica at any of six different inoculum concentrations, nor pathogenicity on intact fruit of four different species or cultivars of cucurbit or hypocotyl tissue of C. maxima cv. Crown, was found to be affected by disruption of the cutA gene.

  17. Comportamiento de dos variedades de sandía Citrullus lanatus (Thunb Matsura y Nakan, injertadas sobre los portainjertos de calabaza, Lagenaria siceraria, zapallo Cucurbita moschata, ahuyama Cucurbita máxima y estropajo Luffa cylindrica The performance of two varieties of water melon Citrullus lanatus (Thub Matsura and nakai, grafted over pumpkin Lagenaria siceraria, zapallo Cucurbita moschata, ahuyama Cucurbita maxima and estropajo Luffa cylindrica graftholders

    Directory of Open Access Journals (Sweden)

    Moreno Juan Carlos

    1992-12-01

    Full Text Available

    En 1987-I, se realizó en predios de la Universidad Nacional de Colombia, Sede Palmira un estudio para evaluar la viabilidad de los injertos, cuantificar la producción e introducir alternativas de manejo de los problemas fitosanitarios. El suelo bien estructurado tiene textura FArL, pH 7.0, materia orgánica 3%, P 48 ppm, K .7 me/100g de suelo y una CIC de 19 me/100g de suelo. Se realizó un injerto de púa o escudete con plántulas de sandía de las variedades Charleston Gray y Sugar baby sobre los patrones de las especies citadas. Al patrón se le eliminó el meristemo apical y se rajó longitudinalmente 1.0 - 1.5 cm y se le introdujo la púa en forma de cuña. La púa consistió en el 50% del tallo, las hojas cotiledonares y el meristemo apical. Las unidades experimentales en el campo se distribuyeron en bloques completos al azar, en donde los tratamientos fueron las plantas injertadas y los tratamientos testigos las variedades de sandía sembradas directamente por semillas. El prendimiento de las púas superó el 90% en todos los casos. Los portainjertos Lagenaria y Cucurbita influyeron en mayor aumento de área foliar, mientras que Luffa disminuyó ostensiblemente. Los portainjertos no desmejoraron las características organolépticas de las frutas de las dos variedades. El número de frutos en las plantas infestadas sobre zapallo y estropajo disminuyeron.

    In the first semester of 1987 was done this experiment at Universidad Nacional, Palmira. The objectives were to evaluate grafts viability, to quantify production and to give alternatives to phytosanitary problems. It was done a prick or escutcheon graft with water melon small plants from Charleston Gray and Sugar Baby. The summit bud was eliminated and it was longitudinally cut 1.0 - 1.5 cm for inserting the prick cotyledon leaves and the summit bud. Experimental units were distributed in random complete blocks, where experiment treatments were grafted plants and control the

  18. 中国南瓜主要性状配合力与遗传力分析%Combining Ability and Heritability of 12 Quantitative Traits in Cucurbita moschata

    Institute of Scientific and Technical Information of China (English)

    王磊; 刘童光; 戴祖云; 施文政; 谈敏; 许晓婷

    2012-01-01

    以12个中国南瓜高世代自交系为试验材料,采用NCⅡ设计配制36个杂交组合,分析了12个数量性状的遗传力、配合力.结果表明:在一般配合力上,6个母本的优劣顺序为Q1>Q3>Q4>Q6>Q2>Q5,6个父本的优劣顺序为B1>B3>B5>B4>B2>B6;亲本的一般配合力与其在杂交组合中的特殊配合力之间无必然联系;主蔓长度、可溶性固形物含量、干物质含量的狭义遗传力较高,早代选择效果较好;节间长度、单果质量、多糖含量、β-胡萝卜素含量、维生素C含量、果实纵径、果实横径的狭义遗传力较低,适合在中高代进行选择.%Thirty six hybrids derived from 12 inbred lines using NC II design were used to study combining ability and heritability of 12 quantitative traits in Cucurbita moschata squash. The results indicated that the general combining ability order of female parent was Q1>Q3> Q4>Q6>Q2>Q5 ,and that of male parents was B1>B3>B5>B4>B2>B6. There was no inevitable corresponding relation between general combining ability of the parent and special combining ability of its hybrid. The main vine length .soluble solid content,dry matter content had higher narrow sense heritability,and they should be selected in early generation. The inter-node length,single fruit weight,polysaccharide content,($-carotene content,Vitamin C content,fruit length,fruit diameter had lower narrow sense heritability,and they should be selected in later generations.

  19. Electron microscopic diagnosis of multiple pathogen of Watermelon mosaic virus and Zucchini yellow mosaic virus in infected Cucurbita moschata%西瓜花叶病毒和小西葫芦黄花叶病毒复合侵染南瓜的透射电镜诊断

    Institute of Scientific and Technical Information of China (English)

    宋西娇; 陈浙; 何步远; 李艳敏; 王荣洲; 吴建祥; 洪健

    2015-01-01

    The multiple infecting pathogens were detected in a sample of yellow mosaic Cucurbita moschata from Longyou, Zhejiang province, by electron microscopy and RT⁃PCR. Filamentous virus particles of 600 ~800 nm in length were observed in Cucurbita moschata by negative staining. The pathological study revealed that III⁃type andⅣ⁃type cylindrical inclusions appeared in cytoplasm of the infected cells. Chloroplast vacuolization and membranous proliferation were also found in the infected cells. There were 622bp and 696bp special productions in gel electrophoresis after RT⁃PCR using universal primers of WMV and ZYMV,respectively. Sequencing after RT⁃PCR revealed that the 622bp and 696bp special productions had homology of 88% ~95% with the WMV sequence and 93%~99% with the ZYMV sequence, respectively. The results above suggested that Cucurbita moschata were multiple⁃infected by WMV and ZYMV.%应用透射电镜和RT⁃PCR技术对浙江龙游的南瓜黄化花叶病株进行病原诊断,电镜负染色观察到病株汁液中存在大量线状病毒粒子,超薄切片观察到病叶细胞质中存在III型、Ⅳ型两种类型的风轮状内含体,细胞质中膜结构增生形成许多小泡结构,叶绿体产生周边空泡。用西瓜花叶病毒( WMV)和小西葫芦黄花叶病毒( ZYMV)特异性引物扩增到622 bp和696 bp目的片段,序列测定发现与WMV和ZYMV 同源性分别为88%~95%、93%~99%。根据以上结果诊断南瓜病株为WMV和ZYMV复合侵染。

  20. HS-SPME-GC-MS Analysis of Volatiles Components in the Pulp and Flesh of Cucurbita moschata from Xinmin of Liaoning Province%辽宁新民金钩南瓜肉和瓤挥发性成分的HS-SPME-GC-MS分析

    Institute of Scientific and Technical Information of China (English)

    张伟; 卢引; 李昌勤; 康文艺

    2012-01-01

    OBJECTIVE: To analyze the volatile components in pulp and flesh of Cucurbita moschata from Xinmin of Liaoning province. METHODS: The samples were analyzed by HS-SPME-GC-MS and Kovats indices, and relative percentage of each component was determined by peak area normalization measurements. RESULTS: 35 compounds were identified from the pulp and 33 from the flesh, accounting for 90.97% and 95.19% of total peak area, respectively. There were 19 same compounds. CONCLUSION: The volatile components are different between the pulp and flesh of C. Moschata. The study can provide reference for the further research of C. Moschata from Xinmin of Liaoning province.%目的:分析辽宁新民金钩南瓜肉和瓤的挥发性成分.方法:采用顶空固相微萃取和气相色谱-质谱联用技术结合保留指数法对样品进行分析,并用峰面积归一化法测定各成分的相对百分含量.结果:从辽宁新民金钩南瓜的果肉和瓤中分别鉴定出35和33个化合物,各占总峰面积的90.97%和95.19%;其中肉和瓤有19个共有成分.结论:辽宁新民金钧南瓜肉和瓤的挥发性成分具有差别.本试验结果可为辽宁新民金钩南瓜的进一步研究提供依据.

  1. Volatile Constituents from Three Parts of Cucurbita Moschata Duch. (Miben) by Head-Space Solid Phase Micro-Extraction Coupled with GC-MS%HS-SPME-GC-MS分析蜜本南瓜3个部位的挥发性成分

    Institute of Scientific and Technical Information of China (English)

    张伟; 彭涛; 卢引; 顾雪竹; 李昌勤; 康文艺

    2013-01-01

    This study was aimed to analyze the volatile constituents from flower, stem tip and seed of Cucurbita moschata Duch.(Miben). The volatiles were analyzed by head-space solid micro-extraction, coupled with GC-MS and Kovats indices for the first time . The results showed that 22 compounds were identified from the flower , 20 from the stem tip and 21 from the seed of the C. moschata (Miben). The total essential constituents from each part were 91 . 89%, 89 . 24% and 96 . 26%, respectively . A total of 10 compounds in the flower and stem tip were mutual. And 3 compounds in the flower, stem tip and seed were mutual. It was concluded that the β-bourbonene (17.57%) and heneicosane (11.90%) were the highest components of the total essential constituents of the flower of C. moschata (Miben). Decanal (28.77%) was the highest components of the stem tip and hexadecanoic acid ethyl ester (29.12%), 2,3-butanediol (16.90%) and linoleic acid ethyl ester (16.52%) were the highest compo-nents of seed of C. moschata (Miben).%目的:对蜜本南瓜花、茎尖和籽的挥发性成分进行分析。方法:采用顶空固相微萃取和气质联用技术(HS-SPME-GC-MS),结合保留指数法,首次分析蜜本南瓜3个部位的挥发性成分。结果:从蜜本南瓜花、茎尖、籽中分别鉴定出22、20和21个挥发性成分,占总峰面积的91.89%、89.24%和96.26%。其中花和茎尖共有10个共有成分,花、茎尖和籽有3个共有成分。结论:蜜本南瓜花中茁-波旁烯(17.57%)和二十一烷(11.90%)含量较高;蜜本南瓜茎尖中癸醛(28.77%)含量最高;籽中棕榈酸乙酯(29.12%)、2,3-丁二醇(16.90%)和亚油酸乙酯(16.52%)含量较高。

  2. [Entomophilic pollination of squash, Cucurbita moschata (Cucurbitaceae)].

    Science.gov (United States)

    Serra, Bruna D V; Campos, Lucio A de O

    2010-01-01

    The objectives of this work were to determine the squash entomofauna in the region of Viçosa, Minas Gerais state, to study their behavior on flowers and their importance for pollination, verifying the role of each pollinator. The most common species were Trigona spinipes (Fabricius), Trigona hyalinata (Lepeletier), Apis mellifera (L.) and Melipona quadrifasciata (Lepeletier). The visitation behavior of A. mellifera, M. quadrifasciata, and Bombus morio (Swederus) were similar. They visited flowers for nectar collection, positioning themselves vertically between the corolla and the sexual structures of the flowers, with the back directed toward the floral axis, which permitted the removal of pollen from the anthers of flowers with stamens and its deposition on the stigma of flowers with pistils, being considered therefore effective pollinators. Trigona spinipes and T. hyalinata foraged in groups, preventing other species from landing on the flowers which they occupied. Due to their small body size and only infrequent contact with the sexual structures of the flowers, these species are considered occasional pollinators. The number of fruits produced differed between freely visited flowers, those prevented from receiving visits and those visited only a single time by M. quadrifasciata, B. morio, A. mellifera, T. hyalinata or T. spinipes. Flowers prevented from receiving visits or visited only once by T. spinipes did not produce fruits. The remaining pollination systems led to fruitification, with open pollination or a single visit from either M. quadrifasciata or B. morio leading to most fruit production.

  3. Study on α-glucosidase inhibitory activity of extracts from six varieties of Cucurbita moschata Duch.%6种栽培品种南瓜提取物的α-葡萄糖苷酶抑制活性的研究

    Institute of Scientific and Technical Information of China (English)

    李昌勤; 卢引; 顾雪竹; 顾海鹏; 康文艺

    2012-01-01

    Objective: To study the α-glucosidase inhibitory activity of extracts from six varieties of Cucurbita moschata Duch. (Jingou, Tianmian, Riben, Chaotianmiben, Miben and Liaoningxinminjingou ) . Methods. By established α-glucosidase inhibitory model in vitro,the activity of extracts from C.moschata was screened.The relationship of inhibitory ratio and the extract concentration were also studied. Results: Different extracts from six varieties of C.moschata all had inhibitory activity of a-glucosidase, among which petroleum ether extract of C.moschata(Jingou) had the highest inhibitor activity of α-glucosidase (IC50 = 143.91μg/mL), which was lower than that of Acarbose( IC50 = 1103.0μg/mL).Among different extracts inhibitor activity of petroleum ether extract was higher than that of ethyl acetate extract and methanol extract.Conclusion:The extracts from six varieties of C. moschata all had inhibitory activity of α-glucosidase, but different varieties of the inhibition rate had certain difference.%目的:对6种不同栽培品种南瓜(金钩、甜面、日本、辽宁新民金钩、超甜蜜本、蜜本)提取物α-葡萄糖苷酶抑制活性进行研究。方法:通过建立体外α-葡萄糖苷酶抑制模型,对南瓜提取物进行活性筛选,并对提取物浓度与抑制活性关系进行研究。结果:6种栽培品种南瓜不同溶剂提取物均有一定的α-葡萄糖苷酶抑制活性,其中,金钩南瓜石油醚提取物的抑制活性最好(IC50=143.91μg/mL),活性远大于阳性对照阿卡波糖(IC50=1103.01μg/mL)。不同溶剂提取物显示石油醚提取物抑制活性均高于乙酸乙酯提取物和甲醇提取物。结论:6种栽培品种南瓜提取物均有一定的α-葡萄糖苷酶抑制活性,但不同品种其抑制活性具有一定的差别。

  4. KARAKTERISTIK SERBUK LABU KUNING (Cucurbita moschata [Characteristic of Pumpkin Powder (Cucurbita moschata

    Directory of Open Access Journals (Sweden)

    Maria O G3

    2005-08-01

    Full Text Available Instant powder of pumpkins was made by mollen dryer in order to extend the product shelf life The aim of the research was to determine the characteristics of the pumpkin powder. The research had been designed using completely randomized block factorial pattern, two levels of drying-up temperature and three levels of mollen dryer speed rotation on two blocks of pumpkin (I and II groups. The parameters measured were percentage of yield, water content, ash, solubility, and sugar, pH, bulk density (g/ml, beta-carotene (µg/g, colors, and hedonic test (scale 1 = not accepted through 5 = really accepted. The research result showed that the drying temperature affected solubility, and the speed rotation affected sugar content of pumpkin powder. The best pumpkin powder was produced by mollen dryer at 60ºC degree and 6 rpm speed rotation which was characterized by high yield and solubility, also produced best hedonic value on taste, color and performance as compared to the other treatment.

  5. Analysis of Genetic Specificity and DNA Fingerprinting Establishment for the Hainan Island Landraces of Cucurbita moschata%中国南瓜海南农家品种间的遗传特异性分析和DNA指纹图谱构建

    Institute of Scientific and Technical Information of China (English)

    云天海; 郑道君; 谢良商; 邓长智

    2013-01-01

    Based on the collection of Hainan island landraces of Cucurbita moschata,the present study was aimed to analyze their genetic specificity and establish DNA fingerprinting for 28 landraces by inter-simple sequence repeat(ISSR) and sequence-related amplified polymorphism (SRAP),which would provide applicable guidance for identification,evaluation,protection,and utilization of Hainan island landraces of C.moschata.The results showed that genetic specificity was significant among the 28 landraces with special genetic background,1O specific markers and 11 unique-lack bands scored were produced by 6 ISSR primers and 11 SRAP primer pairs.And four DNA fingerprintings for the 28 landraces were developed by two ISSR primer combinations and two SRAP primer combinations,i.e.UBC807/UBC814/UBC844/UBC868,UBC808/UBC814/UBC844/UBC868,Me1/Em2 + Me1/Em10 +Me2/Em3,Me1/Em1 + Me1/Em10 + Me8/Em3,respectively.All the DNA fingerprintings developed were intuitionistic and simple.ISSR and SRAP molecular markers could be effectively used in the analysis of genetic specificity and DNA fingerprinting establishment for the landraces of C.moschata.%在收集中国南瓜海南农家品种的基础上,应用ISSR和SRAP标记技术对28份海南农家品种间的遗传特异性进行了分析,并构建指纹图谱,为中国南瓜海南农家品种鉴定、评价、保护和利用提供科学依据.结果表明,所供试的品种间存在显著的遗传特异性,具有特殊的遗传基础或背景,所筛选的6个ISSR引物和11对SRAP引物共产生了10个特异标记和11条唯一缺失带;应用ISSR引物组合UBC807/UBC814/UBC844/UBC868和UBC808/UBC814/UBC844/UBC868,以及SRAP引物组合Me1/Em2+ Me1/Em10+ Me2/Em3和Me1/Em1+Me1/Em10+ Me8/Em3分别绘制了4个28份中国南瓜海南农家品种的DNA指纹图谱,所构建的DNA指纹图谱直观、简单.ISSR标记和SRAP标记技术可有效应用于中国南瓜海南农家品种DNA指纹图谱的构建和遗传特异性鉴定.

  6. Election Districts and Precincts, Duchesne 2, Published in 2002, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Election Districts and Precincts dataset, was produced all or in part from Other information as of 2002. It is described as 'Duchesne 2'. Data by this publisher...

  7. Cucurbita spp. and Cucumis sativus enhance the dissipation of polychlorinated biphenyl congeners by stimulating soil microbial community development.

    Science.gov (United States)

    Qin, Hua; Brookes, Philip C; Xu, Jianming

    2014-01-01

    A number of Cucurbita species have the potential to extract polychlorinated biphenyls (PCBs) from soil, but their impact on the soil microbial communities responsible for PCB degradation remains unclear. A greenhouse experiment was conducted to investigate the effect of three Cucurbita and one Cucumis species on PCB dissipation and soil microbial community structure. Compared to the unplanted control, enhanced losses of PCBs (19.5%-42.7%) were observed in all planted soils. Cucurbita pepo and Cucurbita moschata treatments were more efficient in PCB dissipation, and have similar patterns of soil phospholipid fatty acids (PLFAs) and PCB congener profiles. Cucurbita treatments tend to have higher soil microbial biomass than Cucumis. Gram-negative (G(-)) bacteria were significantly correlated with PCB degradation rates (R(2) = 0.719, p Cucurbita related soil microorganisms could play an important role in remediation of PCB contaminated soils.

  8. Rootstock of interspecific squash hybrids (Cucurbita maxima x Cucurbita moschata) increases lycopene content of watermelon

    Science.gov (United States)

    The watermelon industry in the U.S. is facing increased soil-borne disease pressure and the loss of the soil fumigant methyl bromide. To combat this, grafting of scions with disease-resistant rootstocks of cucurbit species has garnered widespread interest. Both scion and rootstock can affect plant...

  9. Genetic structure and indirect estimates of gene flow in three taxa of Cucurbita (Cucurbitaceae) in western Mexico.

    Science.gov (United States)

    Montes-Hernandez, Salvador; Eguiarte, Luis E

    2002-07-01

    Cultivated squash (Cucurbita argyrosperma ssp. argyrosperma and C. moschata) are important in the Mexican traditional agroecosystem. They are typically cultivated within maize fields where adjacent populations of a wild, close relative, C. argyrosperma ssp. sororia, occur. Consequently, there are ample opportunities for gene flow between domesticated and free-living Cucurbita populations. We used allozymes to examine genetic variation and gene flow among these three Cucurbita taxa in the state of Jalisco in Western Mexico. Twelve polymorphic allozyme loci were used to calculate genetic diversity for 16 populations of Cucurbita. We found high levels of genetic variation: polymorphism of 0.96, mean allelic diversity of 2.08, average expected heterozygosity 0.407, and little differentiation among conspecific populations (D = 0.081; F(ST) = 0.087; N(e)m = 5.22). These findings indicate that Cucurbita possess a high pollen dispersal potential, a somewhat surprising result considering they have specialist pollinators. Unweighted pair group method with arithmetic means (UPGMA) analysis of allozymes suggests the existence of at least two distinct groups of populations, one consisting of both subspecies of C. argyrosperma and another consisting of C. moschata.

  10. Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.

    Science.gov (United States)

    Gong, L; Stift, G; Kofler, R; Pachner, M; Lelley, T

    2008-06-01

    Until recently, only a few microsatellites have been available for Cucurbita, thus their development is highly desirable. The Austrian oil-pumpkin variety Gleisdorfer Olkürbis (C. pepo subsp. pepo) and the C. moschata cultivar Soler (Puerto Rico) were used for SSR development. SSR-enriched partial genomic libraries were established and 2,400 clones were sequenced. Of these 1,058 (44%) contained an SSR at least four repeats long. Primers were designed for 532 SSRs; 500 primer pairs produced fragments of expected size. Of these, 405 (81%) amplified polymorphic fragments in a set of 12 genotypes: three C. moschata, one C. ecuadorensis, and eight C. pepo representing all eight cultivar groups. On an average, C. pepo and C. moschata produced 3.3 alleles per primer pair, showing high inter-species transferability. There were 187 SSR markers detecting polymorphism between the USA oil-pumpkin variety "Lady Godiva" (O5) and the Italian crookneck variety "Bianco Friulano" (CN), which are the parents of our previous F(2) mapping population. It has been used to construct the first published C. pepo map, containing mainly RAPD and AFLP markers. Now the updated map comprises 178 SSRs, 244 AFLPs, 230 RAPDs, five SCARs, and two morphological traits (h and B). It contains 20 linkage groups with a map density of 2.9 cM. The observed genome coverage (Co) is 86.8%.

  11. Phylogenetic relationships among domesticated and wild species of Cucurbita (Cucurbitaceae) inferred from a mitochondrial gene: Implications for crop plant evolution and areas of origin.

    Science.gov (United States)

    Sanjur, Oris I; Piperno, Dolores R; Andres, Thomas C; Wessel-Beaver, Linda

    2002-01-01

    We have investigated the phylogenetic relationships among six wild and six domesticated taxa of Cucurbita using as a marker an intron region from the mitochondrial nad1 gene. Our study represents one of the first successful uses of a mtDNA gene in resolving inter- and intraspecific taxonomic relationships in Angiosperms and yields several important insights into the origins of domesticated Cucurbita. First, our data suggest at least six independent domestication events from distinct wild ancestors. Second, Cucurbita argyrosperma likely was domesticated from a wild Mexican gourd, Cucurbita sororia, probably in the same region of southwest Mexico that gave rise to maize. Third, the wild ancestor of Cucurbita moschata is still unknown, but mtDNA data combined with other sources of information suggest that it will probably be found in lowland northern South America. Fourth, Cucurbita andreana is supported as the wild progenitor of Cucurbita maxima, but humid lowland regions of Bolivia in addition to warmer temperate zones in South America from where C. andreana was originally described should possibly be considered as an area of origin for C. maxima. Fifth, our data support other molecular results that indicate two separate domestications in the Cucurbita pepo complex. The potential zone of domestication for one of the domesticated subspecies, C. pepo subsp. ovifera, includes eastern North America and should be extended to northeastern Mexico. The wild ancestor of the other domesticated subspecies, C. pepo subsp. pepo, is undiscovered but is closely related to C. pepo subsp. fraterna and possibly will be found in southern Mexico.

  12. Agrobiodiversity in Cucurbita spp. landraces collected in Rio de Janeiro assessed by molecular markers

    Directory of Open Access Journals (Sweden)

    Marilene Hilma dos Santos

    2012-01-01

    Full Text Available Diversity and genetic relationship in forty landraces of Cucurbita spp. collected at small farms in Rio de Janeiro, Brazil, were analyzed by RAPD and ISSR markers, using 20 and 15 primers, respectively. Both markers were efficient to cluster the accessions separating among species, but not so much to the detection of intra-specific variability, considering the event of different pairs of accessions comprising null genetic distances observed for both markers in C. moschata. Low values observed for genetic distance among the C. moschata landraces showed that most likely genetic losses is in progress in that region of cultivation due to anthropic and market pressure, which are stimulating the small farmers to abandon their local varieties in order to use commercial seeds, including hybrids, which is causing risk of genetic erosion.

  13. Estudio Farmacognóstico de la droga cruda de la semilla de calabaza (Cucurbita SPP

    Directory of Open Access Journals (Sweden)

    Juan Abreu Payrol

    2001-12-01

    Full Text Available Se presentan algunos parámetros farmacognósticos de la droga cruda de la semilla de calabaza, con vistas a su posterior uso en formulaciones antiparasitarias. Se comparan semillas de 3 variedades: Cucurbita pepo var RG, C. moschata Duch ex Lam Duch ex Porr (Cuba Cueto 8574 y C. maxima var INIVIT C88, en cuanto a masa, humedad residual, cenizas, sustancias solubles y tamizaje fitoquímico, a fin de proponer la mejor para su empleo como antiparasitario, y se trata de forma breve la propuesta de una técnica sencilla para la extracción del principio activo de la semilla (cucurbitina.Some pharmacognostic parameters of the raw drug from the pumpkin seed are presented in order to use it later in antiparasitic formulations. The seeds of 3 varieties are compared: Cucurbita pepo var RG, C. moschata Duch ex Lam Duch ex Porr (Cuba Cueto 8574 and C. maxima var INIVIT C88. Mass, residual humidity, ashes, soluble substances and phytochemical screening were analyzed aimed at proposing the best for its use as antiparasitic. The proposal of a simple technique for the extraction of the active principle of the seed (cucurbitina is briefly explained.

  14. Karakteristik Sosis dengan Fortifikasi β-Caroten dari Labu Kuning (Cucurbita moschata (Sausage Characteristics as Affected by β-Caroten Fortification of Pumpkin (Cucurbita moschata

    Directory of Open Access Journals (Sweden)

    Agus Hadi Prayitno

    2012-02-01

    the filler substitutions on sausage processing. The treatments were: 0% (control, 25, 50, 75, and 100% of the filler. There were five replications in each treatment. The data of physical and chemical characteristics from the completely randomized design were analysed by analysis of variance. The data of sensory characteristics were analysed by the analysis of non parametric test of Hedonic Kruskal-Wallis. The results showed that sausage fortified with β-caroten of pumpkin as the filler substitutions up to 100% level on sausage processing affected significantly (P<0.01 on physical characteristics of sausage (decreased water-holding capacity, increased tenderness and it did not affect pH value of sausage, chemical characteristics of sausage (decreased moisture, increased protein, fiber, β-caroten, and it did not affect the fat content of sausage, and sensory characteristics of sausage (increased taste, decreased color, aroma, texture, touchness, and acceptability of sausage. (Key words: Sausage, Pumpkin, β-caroten, Physical characteristics, Chemical, Sensory

  15. Un ingrediente activo con acción antihelmíntica, a partir de las semillas de Curcubita moschata Duch:estudios analíticos y preformulación

    OpenAIRE

    2007-01-01

    En el presente trabajo se presentan los resultados obtenidos de los estudios analíticos y de preformulación realizados en un sólido pulverulento obtenido a partir de las semillas de Cucurbita moschata Duch mediante un nuevo proceso tecnológico. El ingrediente activo obtenido presenta propiedades físicas, químicas y tecnológicas que permiten su utilización en la elaboración de formas farmacéuticas con acción antihelmíntica. Se evaluó el procedimiento tradicional para la obtención de polvo a pa...

  16. Transcriptome characterization and high throughput SSRs and SNPs discovery in Cucurbita pepo (Cucurbitaceae

    Directory of Open Access Journals (Sweden)

    Nuez Fernando

    2011-02-01

    Full Text Available Abstract Background Cucurbita pepo belongs to the Cucurbitaceae family. The "Zucchini" types rank among the highest-valued vegetables worldwide, and other C. pepo and related Cucurbita spp., are food staples and rich sources of fat and vitamins. A broad range of genomic tools are today available for other cucurbits that have become models for the study of different metabolic processes. However, these tools are still lacking in the Cucurbita genus, thus limiting gene discovery and the process of breeding. Results We report the generation of a total of 512,751 C. pepo EST sequences, using 454 GS FLX Titanium technology. ESTs were obtained from normalized cDNA libraries (root, leaves, and flower tissue prepared using two varieties with contrasting phenotypes for plant, flowering and fruit traits, representing the two C. pepo subspecies: subsp. pepo cv. Zucchini and subsp. ovifera cv Scallop. De novo assembling was performed to generate a collection of 49,610 Cucurbita unigenes (average length of 626 bp that represent the first transcriptome of the species. Over 60% of the unigenes were functionally annotated and assigned to one or more Gene Ontology terms. The distributions of Cucurbita unigenes followed similar tendencies than that reported for Arabidopsis or melon, suggesting that the dataset may represent the whole Cucurbita transcriptome. About 34% unigenes were detected to have known orthologs of Arabidopsis or melon, including genes potentially involved in disease resistance, flowering and fruit quality. Furthermore, a set of 1,882 unigenes with SSR motifs and 9,043 high confidence SNPs between Zucchini and Scallop were identified, of which 3,538 SNPs met criteria for use with high throughput genotyping platforms, and 144 could be detected as CAPS. A set of markers were validated, being 80% of them polymorphic in a set of variable C. pepo and C. moschata accessions. Conclusion We present the first broad survey of gene sequences and allelic

  17. Glucose-lowering activity of novel tetrasaccharide glyceroglycolipids from the fruits of Cucurbita moschata.

    Science.gov (United States)

    Jiang, Zhiguo; Du, Qizhen

    2011-02-01

    Two new tetrasaccharide glyceroglycolipids were obtained from pumpkin. The structures of the two compounds were determined using chemical methods and spectroscopic analysis. Both compounds demonstrated significant glucose-lowering effects in streptozotocin- and high-fat-diet-induced diabetic mice.

  18. Modeling and optimization of ultrasound-assisted extraction of polysaccharide from Cucurbita moschata.

    Science.gov (United States)

    Prakash Maran, J; Mekala, V; Manikandan, S

    2013-02-15

    Polysaccharides from pumpkin were extracted by ultrasound-assisted extraction technology using four factors at five levels central composite rotatable response surface design (CCRD). On using single factor analysis, process variables such as extraction temperature (50-70 °C), power of ultrasound (50-70 W), time (15-25 min) and solid-liquid ratio (1:10-1:20 g/ml) were selected. Experiments were conducted to evaluate the effects of four independent variables on the maximum extraction yield of polysaccharides. From the experimental data, second order polynomial mathematical model were developed with high coefficient of determination values (R(2)>0.96). From response surface plots, temperature and ultrasound power exhibited independent and interactive effects on the extraction yields. Extraction temperature of 70 °C, ultrasound power of 70 W, time of 23 min and solid-liquid ratio of 1:10 g/ml were determined as optimal conditions with a maximum polysaccharides yield of 16.21%, which was confirmed through the validation of the experiments.

  19. [Chemical and biological characterization of meal and protein isolates from pumpkin seed (Cucurbita moschata)].

    Science.gov (United States)

    Salgado, J M; Takashima, M K

    1992-12-01

    The present study was carried out in order to check through chemical and biological analyses the nutritional characteristics of pumpkin seed, its delipidized meal and its proteic concentrate, considering its availability, nutritional potential, facility for production in poor soils and the need for new food resources. Another objective was to complement the amino acid pattern of pumpkin with others protein sources for human consumption. The results obtained indicate that: Raw pumpkin seed meal has a proteic values of 37.6% and the delipidized meal 68.8%; The PER values for raw seed meal and delipidized meal were 2.26 and 1.65, respectively; The chemical composition revealed that the delipized pumpkin seed meal was limited in threonine (66.8%); The isolate and seed meal proteins were both complemented with lysine and with cowpea bean meal; Whole pumpkin seed meal obtained from variety Caravelle is a good caloric material (approximately 568 cal/100 g).

  20. Per se performance of genotypes and correlation analysis in Pumpkin (Cucurbita moschata Duch.ex Poir

    Directory of Open Access Journals (Sweden)

    N.A.Tamil selvi, P.Jansirani, L.Pugalendhi, A.Nirmalakumari

    2012-12-01

    Full Text Available Evaluation of 15 pumpkin genotypes collected from various sources was carried out. Observations were recorded on thefollowing traits viz. vine length, days to first female flower appearance, node number for first female flower appearance, sexratio, days to first harvest, fruit number per vine, fruit weight, fruit equatorial diameter, fruit polar diameter, flesh thickness,seed number per fruit, seed weight per fruit and fruit yield per vine along with quality traits such as total carbohydratescontent, total carotenoids content and crude fibre content of the fruit and analysed statistically. Among the genotypes, CM6followed by CM10 and CM9 recorded the highest mean value of fruit yield. Correlation analysis revealed that fruit yield per vinewas significantly and positively correlated with fruit number per vine, flesh thickness and total carotenoids content.However negative association was observed with days to first female flower appearance, node number for first femaleflower appearance, sex ratio, days to first harvest, fruit weight, fruit equatorial diameter, fruit polar diameter and crude fibrecontent. Hence selecting pumpkin genotypes with narrow sex ratio, more number of fruits per vine, fruits with high flesh thicknessand total carotenoids content will help to improve yield per vine and quality of pumpkin fruits.

  1. Processing and Utilization of Cucurbita moschata Fruit%南瓜的加工利用

    Institute of Scientific and Technical Information of China (English)

    郭安

    2005-01-01

    以南瓜肉为原料,采用不同处理方法,进行3种制品加工工艺的探索研制.结果表明:通过对南瓜的适当处理,将其制成南瓜果脯、南瓜粉、南瓜脆片系列食品是可行的.

  2. Quality Parameters Of Wheat Bread Enriched With Pumpkin (Cucurbita Moschata) By-Products

    OpenAIRE

    Kampuse Solvita; Ozola Liene; Straumite Evita; Galoburda Ruta

    2015-01-01

    Pumpkin processing into puree, juice, candied fruit and pumpkin seed oil results in large amount of by-products. Pumpkins are rich in carotenes, vitamins, minerals, pectin and dietary fibre. The aim of the current study was to evaluate effect of pumpkin pomace and pumpkin residue powder on wheat bread quality. The total content of carotenes was analyzed by spectrophotometric method. The initial increase of pumpkin residue addition indicated increase in loaf volume, which started to decrease a...

  3. Drying characteristics of pumpkin ( Cucurbita moschata) slices in convective and freeze dryer

    Science.gov (United States)

    Caliskan, Gulsah; Dirim, Safiye Nur

    2017-06-01

    This study was intended to determine the drying and rehydration kinetics of convective and freeze dried pumpkin slices (0.5 × 3.5 × 0.5 cm). A pilot scale tray drier (at 80 ± 2 °C inlet temperature, 1 m s-1 air velocity) and freeze drier (13.33 kPa absolute pressure, condenser temperature of -48 ± 2 °C) were used for the drying experiments. Drying curves were fitted to six well-known thin layer drying models. Nonlinear regression analysis was used to evaluate the parameters of the selected models by using statistical software SPSS 16.0 (SPSS Inc., USA). For the convective and freeze drying processes of pumpkin slices, the highest R2 values, and the lowest RMSE as well as χ2 values were obtained from Page model. The effective moisture diffusivity (Deff) of the convective and freeze dried pumpkin slices were obtained from the Fick's diffusion model, and they were found to be 2.233 × 10-7 and 3.040 × 10-9 m2s-1, respectively. Specific moisture extraction rate, moisture extraction rate, and specific energy consumption values were almost twice in freeze drying process. Depending on the results, moisture contents and water activity values of pumpkin slices were in acceptable limits for safe storage of products. The rehydration behaviour of [at 18 ± 2 and 100 ± 2 °C for 1:25, 1:50, 1:75, 1:100, and 1:125 solid:liquid ratios (w:w)] dried pumpkin slices was determined by Peleg's model with the highest R2. The highest total soluble solid loss of pumpkin slices was observed for the rehydration experiment which performed at 1:25 solid: liquid ratio (w:w). Rehydration ratio of freeze dried slices was found 2-3 times higher than convective dried slices.

  4. Effects of season and storage period on accumulation of individual carotenoids in pumpkin flesh (Cucurbita moschata).

    Science.gov (United States)

    Jaswir, Irwandi; Shahidan, Norshazila; Othman, Rashidi; Has-Yun Hashim, Yumi Zuhanis; Octavianti, Fitri; bin Salleh, Mohammad Noor

    2014-01-01

    Carotenoids are antioxidants with pharmaceutical potential. The major carotenoids important to humans are α-carotene, β-carotene, lycopene, lutein, zeaxanthin, and β-cryptoxanthin. Some of the biological functions and actions of these individual carotenoids are quite similar to each other, whereas others are specific. Besides genotype and location, other environmental effects such as temperature, light, mineral uptake, and pH have been found affect carotenoid development in plant tissues and organs. Therefore, this research investigated the effects of the season and storage periods during postharvest handling on the accumulation of carotenoid in pumpkin. This study shows that long-term storage of pumpkins resulted in the accumulation of lutein and β-carotene with a slight decrease in zeaxanthin. The amounts of β-carotene ranged from 174.583±2.105 mg/100g to 692.871±22.019 mg/100g, lutein from 19.841±9.693 mg/100g to 59.481±1.645 mg/100g, and zeaxanthin from not detected to 2.709±0.118 mg/100g. The pumpkins were collected three times in a year; they differed in that zeaxanthin was present only in the first season, while the amounts of β-carotene and lutein were the highest in the second and third seasons, respectively. By identifying the key factors among the postharvest handling conditions that control specific carotenoid accumulations, a greater understanding of how to enhance the nutritional values of pumpkin and other crops will be gained. Postharvest storage conditions can markedly enhance and influence the levels of zeaxanthin, lutein, and β-carotene in pumpkin. This study describes how the magnitudes of these effects depend on the storage period and season.

  5. Drying characteristics of pumpkin (Cucurbita moschata) slices in convective and freeze dryer

    Science.gov (United States)

    Caliskan, Gulsah; Dirim, Safiye Nur

    2017-01-01

    This study was intended to determine the drying and rehydration kinetics of convective and freeze dried pumpkin slices (0.5 × 3.5 × 0.5 cm). A pilot scale tray drier (at 80 ± 2 °C inlet temperature, 1 m s-1 air velocity) and freeze drier (13.33 kPa absolute pressure, condenser temperature of -48 ± 2 °C) were used for the drying experiments. Drying curves were fitted to six well-known thin layer drying models. Nonlinear regression analysis was used to evaluate the parameters of the selected models by using statistical software SPSS 16.0 (SPSS Inc., USA). For the convective and freeze drying processes of pumpkin slices, the highest R2 values, and the lowest RMSE as well as χ2 values were obtained from Page model. The effective moisture diffusivity (Deff) of the convective and freeze dried pumpkin slices were obtained from the Fick's diffusion model, and they were found to be 2.233 × 10-7 and 3.040 × 10-9 m2s-1, respectively. Specific moisture extraction rate, moisture extraction rate, and specific energy consumption values were almost twice in freeze drying process. Depending on the results, moisture contents and water activity values of pumpkin slices were in acceptable limits for safe storage of products. The rehydration behaviour of [at 18 ± 2 and 100 ± 2 °C for 1:25, 1:50, 1:75, 1:100, and 1:125 solid:liquid ratios (w:w)] dried pumpkin slices was determined by Peleg's model with the highest R2. The highest total soluble solid loss of pumpkin slices was observed for the rehydration experiment which performed at 1:25 solid: liquid ratio (w:w). Rehydration ratio of freeze dried slices was found 2-3 times higher than convective dried slices.

  6. Phenotypic stability of traits associated with fruit quality in butternut squash (Cucurbita moschata Duch.

    Directory of Open Access Journals (Sweden)

    Magda Piedad Valdés-Restrepo

    2013-08-01

    Full Text Available The aim of this study was to identify and select genotypes with high fruit dry matter, represented by high starches and carotenes, to obtain a cultivar apt for agroindustrial processes. The model of Eberhart and Russell was used to measure the stability parameter and evaluate the phenotypic stability of traits associated with fruit quality in four populations of butternut squash at three localities in the department of Valle del Cauca (Colombia: Candelaria, Palmira and El Cerrito. Highly significant differences were detected for the genotype x location (P=0.001 interaction for the traits of dry matter, carotene and starch. Except for population 1 in El Cerrito, there was a negative effect on the accumulation of photosynthates at 1,536 m a.s.l., probably due to the low quality of the heliophany. The populations 1, 2 and 3 were stable and predictable, complying with the parameters β = 1 and d = 0 of the Eberhart and Russell methodology. Population 1 was notable in terms of high fruit production, high dry matter content and good phenotypic stability

  7. MANFAAT BIJI LABU (Cucurbita sp. UNTUK KESEHATAN

    Directory of Open Access Journals (Sweden)

    Djoko Hargono

    2012-09-01

    Full Text Available Daging buah C. moschata Duch. ex Poir yang telah masak dapat dimakan setelah dikukus, disayur atau dijadikan kolak. Daun tumbuhan yang muda dan kuncup bunganya dimakan sebagai sayuran. Bijinya setelah diasin dan dikeringkan di sinar matahari atau cara lain dikonsumsi sebagai kuaci. Daging buah dan biji C. pepo L dan C. maxima Duch dimakan setelah diolah seperti daging buah dan biji C. moschata Duch. ex Poir. Secara tradisional biji C. moschata Duch ex Poir digunakan sebagai obat kecacingan, terutama untuk cacing pita.

  8. High Throughput Sequencing of Small RNAs in the Two Cucurbita Germplasm with Different Sodium Accumulation Patterns Identifies Novel MicroRNAs Involved in Salt Stress Response.

    Directory of Open Access Journals (Sweden)

    Junjun Xie

    Full Text Available MicroRNAs (miRNAs, a class of small non-coding RNAs, recognize their mRNA targets based on perfect sequence complementarity. MiRNAs lead to broader changes in gene expression after plants are exposed to stress. High-throughput sequencing is an effective method to identify and profile small RNA populations in non-model plants under salt stresses, significantly improving our knowledge regarding miRNA functions in salt tolerance. Cucurbits are sensitive to soil salinity, and the Cucurbita genus is used as the rootstock of other cucurbits to enhance salt tolerance. Several cucurbit crops have been used for miRNA sequencing but salt stress-related miRNAs in cucurbit species have not been reported. In this study, we subjected two Cucurbita germplasm, namely, N12 (Cucurbita. maxima Duch. and N15 (Cucurbita. moschata Duch., with different sodium accumulation patterns, to Illumina sequencing to determine small RNA populations in root tissues after 4 h of salt treatment and control. A total of 21,548,326 and 19,394,108 reads were generated from the control and salt-treated N12 root tissues, respectively. By contrast, 19,108,240 and 20,546,052 reads were obtained from the control and salt-treated N15 root tissues, respectively. Fifty-eight conserved miRNA families and 33 novel miRNAs were identified in the two Cucurbita germplasm. Seven miRNAs (six conserved miRNAs and one novel miRNAs were up-regulated in salt-treated N12 and N15 samples. Most target genes of differentially expressed novel miRNAs were transcription factors and salt stress-responsive proteins, including dehydration-induced protein, cation/H+ antiporter 18, and CBL-interacting serine/threonine-protein kinase. The differential expression of miRNAs between the two Cucurbita germplasm under salt stress conditions and their target genes demonstrated that novel miRNAs play an important role in the response of the two Cucurbita germplasm to salt stress. The present study initially explored small

  9. High Throughput Sequencing of Small RNAs in the Two Cucurbita Germplasm with Different Sodium Accumulation Patterns Identifies Novel MicroRNAs Involved in Salt Stress Response.

    Science.gov (United States)

    Xie, Junjun; Lei, Bo; Niu, Mengliang; Huang, Yuan; Kong, Qiusheng; Bie, Zhilong

    2015-01-01

    MicroRNAs (miRNAs), a class of small non-coding RNAs, recognize their mRNA targets based on perfect sequence complementarity. MiRNAs lead to broader changes in gene expression after plants are exposed to stress. High-throughput sequencing is an effective method to identify and profile small RNA populations in non-model plants under salt stresses, significantly improving our knowledge regarding miRNA functions in salt tolerance. Cucurbits are sensitive to soil salinity, and the Cucurbita genus is used as the rootstock of other cucurbits to enhance salt tolerance. Several cucurbit crops have been used for miRNA sequencing but salt stress-related miRNAs in cucurbit species have not been reported. In this study, we subjected two Cucurbita germplasm, namely, N12 (Cucurbita. maxima Duch.) and N15 (Cucurbita. moschata Duch.), with different sodium accumulation patterns, to Illumina sequencing to determine small RNA populations in root tissues after 4 h of salt treatment and control. A total of 21,548,326 and 19,394,108 reads were generated from the control and salt-treated N12 root tissues, respectively. By contrast, 19,108,240 and 20,546,052 reads were obtained from the control and salt-treated N15 root tissues, respectively. Fifty-eight conserved miRNA families and 33 novel miRNAs were identified in the two Cucurbita germplasm. Seven miRNAs (six conserved miRNAs and one novel miRNAs) were up-regulated in salt-treated N12 and N15 samples. Most target genes of differentially expressed novel miRNAs were transcription factors and salt stress-responsive proteins, including dehydration-induced protein, cation/H+ antiporter 18, and CBL-interacting serine/threonine-protein kinase. The differential expression of miRNAs between the two Cucurbita germplasm under salt stress conditions and their target genes demonstrated that novel miRNAs play an important role in the response of the two Cucurbita germplasm to salt stress. The present study initially explored small RNAs in the

  10. Host plant oviposition preference of Bactrocera cucurbitae (Coquillett) (Diptera : Tephritidae)%瓜实蝇对寄主植物的产卵选择性

    Institute of Scientific and Technical Information of China (English)

    彭帅; 郑丽霞; 吴伟坚

    2013-01-01

    The melon fruit fly, Bactrocera cucurbitae ( Coquillett) has been reported to damage more than 100 host plants and is a major pest of cucurbitaceous vegetables. Choice oviposition experiments were conducted under laboratory conditions to help assess host plant specificity of the fruit fly. Six host species: Momordica charantiap, Luffa cylindrical, Cucumis sativus, Cucurbita moschata, Carica papaya and Cucurbita pepo were tested. Oviposition rates were highest on M. charantiap (56. 18% of eggs) and C. sativus (29. 00% ) , with low rates on C. papaya (1. 85% ) and C. moschata (0. 00% ) . In contrast, Oviposition rates were highest on C. sativus ( 34. 64% ) , C. pepo ( 30. 37% ) and C. moschata (18. 05% ) , with low rates on C. papaya (0. 60% ) when the fruit peel were pared. Oviposition rates of B. cucurbitae females on fruits with peel had a significant negative correlation with the hardness of peel, and the correlation coefficient was - 0. 8772. The results imply that in addition to the in - built chemical compounds, plants have physical barrier like hard fruit peel, which impact melon fruit fly oviposition.%瓜实蝇Bactrocera cucurbitae (Coquillett)可危害100多种寄主植物,是葫芦科蔬菜的重要害虫.为了探明瓜实蝇的寄主特异性,在实验室条件下研究了瓜实蝇对黄瓜Cucumis sativus、苦瓜Momordica charantiap、丝瓜Luffa cylindrica、南瓜Cucurbita moschata、番木瓜Carica papaya和西葫芦Cucurbita pepo的产卵选择性.瓜实蝇在苦瓜和黄瓜上的产卵比率较高,分别为56.18%和29.00%,在番木瓜和南瓜上产卵比率较低,分别为1.85%和0.00%,但是在去掉果皮后,黄瓜、西葫芦和南瓜的产卵比率较高,分别达到34.64%、30.37%和18.05%,在番木瓜上的产卵率较低仅为0.60%.瓜实蝇雌虫在带皮果实上的产卵比率与果皮硬度呈显著的负相关关系,相关系数为-0.8772结果表明除了自身的化合物之外,植物具有的物理屏障如坚硬的

  11. Biology of Anastrepha grandis (Diptera: Tephritidae) in Different Cucurbits.

    Science.gov (United States)

    Bolzan, Anderson; Nava, Dori E; Garcia, Flávio R M; Valgas, Ricardo A; Smaniotto, Giovani

    2015-06-01

    Anastrepha grandis (Macquart) (Diptera: Tephritidae) is one of the main pests of cucurbits in Brazil. Losses occur due to the damage caused to the fruits and the embargo on exports, as A. grandis is considered a quarantine pest in countries that import Brazilian cucurbits. This study aimed to evaluate the development of A. grandis in hosts of the Cucurbitaceae family. The hosts used were stem squash (Cucurbita pepo L.), squash (Cucurbita moschata Duchesne), chayote [Sechium edule (Jacq.) Swartz], mini watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai], Spanish melon (Cucumis melo L.), hybrid squash "Tetsukabuto" (C. moschata×Cucurbita maxima Duchesne), and salad cucumber (Cucumis sativus L.). We evaluated the viability and duration of egg-to-pupa period, pupal weight, sex ratio, and average number of pupae per fruit under controlled conditions of temperature, relative humidity, and photophase. The preoviposition and oviposition periods, fecundity, fertility, and longevity of females were determined for adults. Hosts of the genus Cucurbita provided a better development of A. grandis in comparison with other hosts, and presented a greater number of insects on fruit as well as higher infestation rate. Fecundity and longevity were also higher for females that developed in hosts of the genus Cucurbita, although values of these biological parameters varied between stem squash, squash, hybrid squash "Tetsukabuto."

  12. 西洋南瓜和中国南瓜引种栽培试验%Introduction of Pumpkin Cultivars Cucurbita maxima and Cucurbita moschata

    Institute of Scientific and Technical Information of China (English)

    崔群香; 张爱慧; 朱丽梅; 朱凌丽; 朱士农

    2007-01-01

    在江苏南京地区对引种的5个西洋南瓜和6个中国南瓜品种进行了春秋两季栽培,结果表明:万寿、绿彩2002、金彩2002、富贵甜栗、镇江青板栗等5个西洋南瓜品种适宜春季栽培,栽培过程中宜采用适宜的整枝方式.中国南瓜品种中蜜本南瓜座果率高,品质好,产量高,抗病虫,春秋两季均可栽培;黄狼南瓜、徐-1、徐-2、盐-1、宁-1等5个品种适宜春季粗放栽培,兼食南瓜花和南瓜茎.

  13. 砍瓜×广西蜜本南瓜的杂种鉴定%Identification of Hybrid F1 of Cucurbita kangua Li. × Cucurbita moschata Duch.

    Institute of Scientific and Technical Information of China (English)

    李晓林; 韦静宜; 叶绍华

    2007-01-01

    采用聚丙烯酰胺凝胶垂直平板电泳法对砍瓜(母本)、广西蜜本南瓜(父本)以及杂种F1代的POD与Est同工酶进行测定,结果表明,父母本的两种同工酶均有明显差异,F1代两种同工酶谱表现出双亲的互补性,F1代Est同工酶出现杂种酶带Rf=0.816.证明POD和Est同工酶是可以用来鉴定南瓜品种及其杂种.气孔密度和形态也反应出双亲与F1代的差异,F1代气孔密度介于双亲之间,气孔形态偏向于父本.因此,气孔形态特征也可用来鉴定双亲和杂种.F1代瓜皮、瓜肉颜色和品质更接近父本.

  14. DEPRESIÓN EN VIGOR POR ENDOGAMIA Y HETEROSIS PARA EL RENDIMIENTO Y SUS COMPONENTES EN ZAPALLO Cucurbita moschata Duch. Ex Poir INBREEDING DEPRESSION AND HETEROSIS FOR YIELD AND ITS COMPONENTS IN PUMPKIN Cucurbita moschata Duch. Ex Poir

    Directory of Open Access Journals (Sweden)

    Miguel Mariano Espitia Camacho

    2006-06-01

    Full Text Available El estudio tuvo como objetivos estimar la depresión por endogamia (DE y cuatro tipos de heterosis: promedio general (HP, promedio de progenitores (HM, heterobeltiosis (HB y útil (HU, en dos dialélos [uno entre cinco variedades (V y el otro entre cinco líneas endogamicas S1 (LS1, derivadas de éstas], para el rendimiento por planta (PFP, número de frutos por planta (NFP y peso promedio de fruto (PPF. Los 30 genotipos se evaluaron en el municipio de Candelaria (Valle del Cauca, Colombia, en un diseño de bloques completos al azar con cinco repeticiones. Se presentaron diferencias significativas (p 40 % y HU (>60 % en PFP.With the goal of estimating inbreeding depression (DE and the four types of heterosis: general average (HP, mean (HM, heterobeltiosis (HB and standard (HU, in two diallelic [one among five cross pollinated varieties (VAR and the other among five self-pollinated lines S1(LS1, derived from these], for yield/plant (PFP, number of fruits/plant (NFP and weight/fruit (PPF. The 30 genotypes were evaluated using a complete randomized design with five replicates in Candelaria (Valle del Cauca, Colombia . Significant differences (p 40 % and HU (>60 % in PFP.

  15. EFECTOS HETERÓTICOS Y HABILIDAD COMBINATORIA PARA EL RENDIMIENTO POR PLANTA EN Cucurbita moschata DUCH. Ex Poir HETEROTIC EFFECTS AND COMBINING ABILITY FOR YIELD PER PLANT IN Cucurbita moschata DUCH. Ex Poir

    Directory of Open Access Journals (Sweden)

    Miguel Mariano Espitia Camacho

    2006-06-01

    Full Text Available El estudio tuvo como objetivos estimar los efectos heteróticos y de habilidad combinatoria (HCG y específica (HCE, para el rendimiento/planta (RFP, en dos dialelos [uno entre cinco variedades (VAR y el otro entre cinco líneas endogámicas S1 (LS1, derivadas de éstas], mediante las metodologías propuestas por Gardner y Eberhart (GyE: 1966: Análisis II y Hallauer y Miranda (HyM:1981. Los genotipos se evaluaron en Candelaria (Valle del Cauca, Colombia, en un diseño de bloques completos al azar con cinco repeticiones. Las diferencias entre genotipos en el dialelo de LS1 por GyE, fueron explicadas por los efectos de variedades (v j: p jj’: p : p The purpose of this study was to estimate the heterotic effects, general combining ability (GCA, and specific (SCA for fruit yield per plant (RFP in two diallelic [one among five cross pollinated varieties (VAR and the other among five self-pollinated lines S1 (LS1, derived from these], according to the methodologies designed by Gardner y Eberhart (GyE: 1966: Analysis II and Hallauer and Miranda (HyM: 1981 using a complete randomized design with five replicates in Candelaria (Valle del Cauca, Colombia. The differences between genotypes observed in the diallelic of LS1 by GyE, were explained by the effects of varieties (v j: p (h jj’: p : p < 0,01 and specific heterosis (Sjj’: p < 0,05 explained the differences for h jj in the diallelic of LS1. According to the methodology of HyM, the additive (GCA: p < 0,01 and non additive genic action (SCA: p < 0,05 controlled the expression of RFP in the diallele of LS1 with greater importance of the GCA. In the diallele of VAR, only the GCA (p < 0,01 explained such control. The genotypes Bolo Verde (RFP = 17,8 kg/plant; v j = 3,34**; g i = 1,73* and Soler (PFP = 17 kg/plant; v j = 2,89*; g i = 175* are the recommended genotypes to exploit the additives effects and the hybrid LA34 x LSO (RFP = 26 kg/plant; Sjj’= 3,55** to exploit the additive and non additive effects through reciprocal recurrent selection.

  16. 无公害南瓜生产技术%Pollution-free pumpkin (Cucurbita moschata) production technology

    Institute of Scientific and Technical Information of China (English)

    冯成玉; 于宝富

    2005-01-01

    南瓜是一种营养丰富同时具有保健功能的蔬菜,通常以食用其嫩瓜或老熟瓜为主.由于南瓜生长期间基本不需使用农药,是深受消费者青睐的无公害蔬菜.为满足市场需求,我们结合农业结构调整,成功地摸索出一套南瓜无公害生产模式,取得了显著成效.

  17. Crystallization and preliminary X-ray analysis of the glycerol-3-phosphate 1-acyltransferase from squash (Cucurbita moschata).

    Science.gov (United States)

    Turnbull, A P; Rafferty, J B; Sedelnikova, S E; Slabas, A R; Schierer, T P; Kroon, J T; Nishida, I; Murata, N; Simon, J W; Rice, D W

    2001-03-01

    Glycerol-3-phosphate 1-acyltransferase (E.C. 2.3.1.15; G3PAT) catalyses the incorporation of an acyl group from either acyl-acyl carrier proteins (acylACPs) or acylCoAs into the sn-1 position of glycerol 3-phosphate to yield 1-acylglycerol 3-phosphate. Crystals of squash G3PAT have been obtained by the hanging-drop method of vapour diffusion using PEG 4000 as the precipitant. These crystals are most likely to belong to space group P2(1)2(1)2(1), with approximate unit-cell parameters a = 61.1, b = 65.1, c = 103.3 A, alpha = beta = gamma = 90 degrees and a monomer in the asymmetric unit. X-ray diffraction data to 1.9 A resolution have been collected in-house using a MAR 345 imaging-plate system.

  18. Molecular Characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite Naturally Infecting Pumpkin (Cucurbita moschata) in India.

    Science.gov (United States)

    Namrata, Jaiswal; Saritha, R K; Datta, D; Singh, M; Dubey, R S; Rai, A B; Rai, M

    2010-10-01

    Pumpkin cultivation in India is affected by severe incidence of a yellow vein mosaic disease. Tomato leaf curl New Delhi virus and Squash leaf curl China virus are known to be associated with this disease in India. We were able to identify a third begomovirus-Tomato leaf curl Palampur virus (ToLCPMV), from pumpkin showing typical symptoms of the disease at Varanasi based on the sequence of complete DNA-A genome of the virus. The complete DNA-A sequence of the virus shared more than 99% sequence identity with other ToLCPMV isolates available in the GenBank and clustered with them in the phylogenetic analysis. This betasatellite amplified from the same infected sample has been identified as Pepper leaf curl betasatellite (PepLCB) which also infects chilli in India. There was 92% sequence identity between the two isolates. This is the first report of natural infection of ToLCPMV on pumpkin and association of PepLCB with yellow vein mosaic disease of pumpkin in India.

  19. Nutritional evaluation of phosphorylated pumpkin seed (Cucurbita moschata) protein concentrate in silver catfish Rhamdia quelen (Quoy and Gaimard, 1824).

    Science.gov (United States)

    Lovatto, Naglezi de Menezes; Goulart, Fernanda Rodrigues; de Freitas, Silvandro Tonetto; Mombach, Patricia Inês; Loureiro, Bruno Bianch; Bender, Ana Betine Beutinger; Boligon, Aline Augusti; Radünz Neto, João; da Silva, Leila Picolli

    2015-12-01

    An 8-week feeding trial was conducted to evaluate the effect of replacing fish meal with pumpkin seed meal (PSM) or phosphorylated protein concentrate of pumpkin seed meal (PPCPS) on growth and metabolic responses of silver catfish. Five isonitrogenous and isocaloric diets were formulated. Control diet contained fish meal as the main protein source. The treatment groups contained 25 and 50% of either PSM or PPCPS protein replaced the fishmeal protein. A total of 400 silver catfish, with initial mean weight of 24 ± 0.46 g, were distributed into 20 tanks. For data four orthogonal contrasts were applied: control diet versus PSM diets; control diets versus PPCPS diets; control versus other diets; PSM diets versus PPCPS diets. The results indicated that the fish fed PSM diets had lower weight gain when compared to either control diet or PPCPS. The PPCPS do not affect growth and protein efficiency ratio. Lower albumin contents were found for the control diet fish for the contrasts control diet versus PPCPS diet and control diet versus other diets. The hepatic ALAT enzyme activity was higher in the fish fed the control diet (P < 0.05). The hepatic ALP was most active in fish that received the PPCPS diets, when comparing control diet versus PPCPS diets and control diet versus other diets. The hepatosomatic index was higher for fish fed the PPCPS. Our results indicated that PPCPS presents relevant nutritional quality for fish and can replace the fish meal protein up to 50% without affecting growth, PER and intermediate metabolites in silver catfish.

  20. The Effect of Butternut Squash (Cucurbita moschata) on the Incomes of the Small-Holders in Suba District, Kenya

    OpenAIRE

    H. N. Isaboke; P. Mshenga; M. Saidi

    2012-01-01

    Butternut squash is an emerging economic crop in Kenya with ready market and high nutritional value. It was introduced to promote food security and enhance the incomes of the small-holder farmers in Suba district. However it is not clear whether butternut could address the problem of low income among small-holder farmers. The aim of the study was to investigate the effect of butternut squash on the incomes of the small-holders. The choice of the study area was due to persistence of poverty pr...

  1. 南瓜化学成分的研究%Study on Chemical Constituents of Cucurbita Moschata Duch

    Institute of Scientific and Technical Information of China (English)

    王岱杰; 杜琪珍; 王晓; 耿岩玲; 张立金; 段文娟; 傅茂润; 葛长军

    2010-01-01

    目的 研究南瓜的化学成分.方法 采用乙醇、氯仿提取,硅胶、Sephadex LH-20柱色谱分离,通过理化常数和光谱分析鉴定化合物的结构.结果 从南瓜的氯仿提取物中分离并鉴定了6个化合物:β-谷甾醇(β-sitosterol,Ⅰ)、顺-15-十八烯酸(cis-15-vaccenic acid,Ⅱ)、硬脂酸(stearic acid,Ⅲ)、顺-15-十八烯酸甲酯(cis-15-vacxenic acid methyl ester,Ⅳ)、大豆脑苷Ⅰ(soya-cerebroside Ⅰ,Ⅴ)和蔗糖(sucrose,Ⅵ).结论 6个化合物均系首次从南瓜中分离得到.

  2. Nutrient Content And Acceptability Of Snakehead-Fish (Ophiocephalus Striatus) And Pumpkin (Cucurbita Moschata) Based Complementary Foods

    Science.gov (United States)

    Ratna Noer, Etika; Candra, Aryu; Panunggal, Binar

    2017-02-01

    Poor nutrient-dense complementary foods is one of the common factors contributed for decline growth pattern in children. Snakehead-fish and Pumpkin Complementary Feeding (SPCF) base on locally food can help to reduce child malnutrition. Specifically, high protein and vitamin A in SPCF may improve immunity and nutrition status of malnutrition children. This study aimed to formulate low-cost, nutritive value and acceptable of SPCF on malnutrition children in coastal area. Carbohydrate content was determined by difference, protein by Kjeldahl, betacaroten by spectofotometri and sensory evaluation using a five point hedonic scale. Fe and zinc was determined by AAS. There is an effect of the substitution of snake-head fish flour and yellow pumpkin flour toward the nutrient content and the acceptability

  3. Efeito do tratamento térmico em sementes de abóboras (Cucurbita spp.) sobre os níveis de fatores antinutricionais e/ou tóxicos Effect of the thermal processing on pumpkin seeds (Cucurbita spp.) on the levels of antinutritionales factors and/or toxics

    OpenAIRE

    Glauciemar Del-Vechio; Angelita Duarte Corrêa; Celeste Maria Patto de Abreu; Custódio Donizete dos Santos

    2005-01-01

    Os teores de alguns antinutrientes de sementes cruas, cozidas e tostadas de três espécies de abóboras, Cucurbita maxima (CMA), C. moschata (CMO) e o híbrido F1 (CMA X CMO), foram investigados, a fim de assegurar o seu uso em preparações dietéticas, produtos industrializados e formulações de novos produtos. Não foram detectados em nenhuma das espécies estudadas teores de ácido oxálico e nitrato. A espécie C. maxima apresentou os níveis mais baixos de cianeto e de polifenóis e maior digestibili...

  4. Efeito do tratamento térmico em sementes de abóboras (Cucurbita spp. sobre os níveis de fatores antinutricionais e/ou tóxicos Effect of the thermal processing on pumpkin seeds (Cucurbita spp. on the levels of antinutritionales factors and/or toxics

    Directory of Open Access Journals (Sweden)

    Glauciemar Del-Vechio

    2005-04-01

    Full Text Available Os teores de alguns antinutrientes de sementes cruas, cozidas e tostadas de três espécies de abóboras, Cucurbita maxima (CMA, C. moschata (CMO e o híbrido F1 (CMA X CMO, foram investigados, a fim de assegurar o seu uso em preparações dietéticas, produtos industrializados e formulações de novos produtos. Não foram detectados em nenhuma das espécies estudadas teores de ácido oxálico e nitrato. A espécie C. maxima apresentou os níveis mais baixos de cianeto e de polifenóis e maior digestibilidade protéica in vitro. O cozimento acarretou redução nos níveis de cianeto, inibidor de tripsina, da atividade de hemaglutinina e de polifenóis e aumento da digestibilidade protéica in vitro. Dos tratamentos térmicos utilizados, o cozimento foi o mais eficiente na redução desses constituintes.The contents of some antinutrients of raw, boiled and toasted seeds of three pumpkin species, Cucurbita maxima (CMA, C. moschata (CMO and the F1 hybrid (CMA x CMO, were investigated in order to assure the use in dietary preparations industrialized products and formulations of new products. Levels of oxalic acid and nitrate were not detected in any of the species studied. CMA presented the lowest levels of cyanide, polyphenols and highest in vitro protein digestibility. Cooking brought about reduction in the levels of cyanide, trypsin inhibitor, hemaglutinin activity and polyphenols and increased of in vitro protein digestibility. Of the thermal processing employed, cooking was the most efficient in reducing of that constituints.

  5. Novel calicivirus from a ferret badger (Melogale moschata) in China.

    Science.gov (United States)

    Miao, Fa-Ming; Li, Yue-Hong; Liu, Ye; Zhang, Shou-Feng; Miao, Fu-Chun; Zhao, Jing-Hui; Hu, Rong-Liang

    2015-07-01

    We describe the isolation and complete genome sequence of a new calicivirus, FBCV-JX12, isolated from a ferret badger (Melogale moschata). Comparison of FBCV-JX12 with other vesiviruses revealed that it shared the highest amino acid sequence identities of 71.6, 60.5, and 59.3% in the nonstructural protein, VP1, and VP2, respectively, with MCV-DL2007 (mink calicivirus). Phylogenetic analysis of the whole genomic sequence showed that it clustered most closely with MCV-DL2007 of the genus Vesivirus, but with low nucleotide similarity in the three open reading frames (62.1-68.5%).

  6. Eficiência de acessos de Cucurbita maxima como polinizadores de abóbora híbrida do tipo "Tetsukabuto" Efficiency of Cucurbita maxima accessions as pollinators in the commercial production of pumpkin ("Tetsukabuto" type

    Directory of Open Access Journals (Sweden)

    Warley Marcos Nascimento

    2008-12-01

    Full Text Available A abóbora tipo “Tetsukabuto” é um híbrido interespecífico, macho estéril, resultante do cruzamento entre Cucurbita maxima e C. moschata. Uma das estratégias utilizadas para produção comercial de frutos de “Tetsukabuto” tem sido o plantio adjacente e concomitante de acessos de C. maxima ou C. moschata como polinizadores. Este trabalho teve por objetivo avaliar a eficiência de diferentes acessos de C. maxima como fontes de pólen para produção comercial de abóbora tipo “Tetsukabuto”. A cultivar de moranga "Exposição" e o acesso "Nirvana" foram utilizados como polinizadores para produção de frutos de dois híbridos do tipo varietal “Tetsukabuto” ("Jabras" e "Kyoto". Foram avaliados os tratamentos (cruzamentos Jabras x Exposição, Jabras x Nirvana, Tetsukabuto x Exposição, Tetsukabuto x Nirvana, em quatro repetições. Foram determinados os parâmetros massa dos frutos, diâmetro dos frutos, espessura da polpa, número e massa de sementes. O acesso "Nirvana" (cultivar em fase de validação pode ser utilizado na produção comercial como polinizador de abóboras deste grupo varietal sem nenhum prejuízo quando comparado com a moranga "Exposição"."Tetsukabuto" pumpkins are interspecific hybrids between Cucurbita maxima x C. moschata accessions. Such hybrids, however, are often male-sterile due to the impaired ability of the staminate flowers to produce functional pollen. The use of intervening rows of staminate (pollen-donor plants is one strategy employed in order to have commercial fruit production. In the present work, two C. maxima accessions ["Exposição" (E and "Nirvana" (N] were employed as pollen-donors (staminate parents and their efficiency was compared considering fruit and seed yield parameters. Two pistillate Tetsukabuto hybrids ["Jabras" (J and "Kyoto Tetsukabuto" (KT] were employed in four possible unidirectional crosses (J x E, J x N, KT x E and KT x N. Fruits were produced using standard manual

  7. Phytoaccumulation of antimicrobials by hydroponic Cucurbita pepo.

    Science.gov (United States)

    Aryal, Niroj; Reinhold, Dawn

    2013-01-01

    Consumer use of antimicrobial-containing products continuously introduces triclocarban and triclosan into the environment. Triclocarban and triclosan adversely affect plants and animals and have the potential to affect human health. Research examined the phytoaccumulation of triclocarban and triclosan by pumpkin (Cucurbita pepo cultivar Howden) and zucchini (Cucurbita pepo cultivar Gold Rush) grown hydroponically. Pumpkin and zucchini were grown in nutrient solution spiked with 0.315 microg/mL triclocarban and 0.289 microg/mL triclosan for two months. Concentrations of triclocarban and triclosan in nutrient solutions were monitored weekly. At the end of the trial, roots and shoots were analyzed for triclocarban and triclosan. Research demonstrated that pumpkin and zucchini accumulated triclocarban and triclosan. Root accumulation factors were 1.78 and 0.64 and translocation factors were 0.001 and 0.082 for triclocarban and triclosan, respectively. The results of this experiment were compared with a previous soil column study that represented environmentally relevant exposure of antimicrobials from biosolids and had similar root mass. Plants were not as efficient in removing triclocarban and triclosan in hydroponic systems as in soil systems. Shoot concentrations of antimicrobials were the same or lower in hydroponic systems than in soil columns, indicating that hydroponic system does not overpredict the concentrations of antimicrobials.

  8. Genetics of oil pumpkin, Cucurbita pepo L.

    Directory of Open Access Journals (Sweden)

    Berenji Janoš

    2011-01-01

    Full Text Available Oil pumpkin (Cucurbita pepo L. belongs to alternative crops grown for seeds and high quality seed oil. One of the basic prerequisites of successful production of oil pumpkin is the proper choice of cultivar. The genetics of different traits of oil pumpkin represents the basis for breeding of new cultivars of oil pumpkin. Of special interest for oil pumpkin breeding are the genetics of seed- coat, plant growth type, resistance to diseases and genetic basis of male sterility. For practical breeding of oil pumpkin the theory of monogenic inheritance of seed coat is quite satisfactory. In light of the theory of developmental reversal of dominance, it may be concluded for C. pepo that the bush type of growth is almost completely dominant over the vine habit at the beginning of plant growth, be- coming incompletely dominant in the second part of the season. In C. moshata several resistance genes of interest in oil pumpkin breeding for virus resistance were discovered. Male sterility, which was transferred to different genotypes of naked seeded oil pumpkin has potential for production of F1 hybrid seed. In addition to traits mention above, other genes of interest for genetics and breeding of oil pumpkin are also mentioned in this paper.

  9. Phytolith evidence for early Holocene Cucurbita domestication in southwest Ecuador.

    Science.gov (United States)

    Piperno, Dolores R; Stothert, Karen E

    2003-02-14

    Cucurbita (squash and gourd) phytoliths recovered from two early Holocene archaeological sites in southwestern Ecuador and directly dated to 10,130 to 9320 carbon-14 years before the present (about 12,000 to 10,000 calendar years ago) are identified as derived from domesticated plants because they are considerably larger than those from modern wild taxa. The beginnings of plant husbandry appear to have been preceded by the exploitation of a wild species of Cucurbita during the terminal Pleistocene. These data provide evidence for an independent emergence of plant food production in lowland South America that was contemporaneous with or slightly before that in highland Mesoamerica.

  10. Biological control of Cucurbita pepo var texana (Texas gourd) in cotton (Gossypium hirsutum) with the fungus Fusarium solani f sp Cucurbitae

    Science.gov (United States)

    Experiments were conducted to evaluate various formulations and application methods of the fungus Fusarium solani f. sp. cucurbitae (FSC) for controlling Texas gourd (Cucurbita pepo var. texana) in cotton (Gosssypium hirsutum). In greenhouse tests, Texas gourd was controlled 93% and 96%, respective...

  11. Plant pigments (antioxidants) of medicinal plants Malva silvestris L. and Malva moschata L. (Malvaceae).

    Science.gov (United States)

    Redzić, Sulejman; Hodzić, Nizama; Tuka, Mijat

    2005-05-01

    Qualitative-quantitative structure of plant pigments in wild plants Malva silvestrs L. and Malva moschata L. (Malvaceae), which were collected in 20 locations in Sarajevo area and surroundings, was tested during spring and summer in 2003. Acetone extracts of both categories were made and rising paper-chromatography done for the purpose of qualitative analysis. Quantitative analysis was done by spectrophotometry. Chlorophyll a, chlorophyll b and xanthophylls presence was confirmed by separation of pigments from acetone extract of these plant species. Spectrophotometric analysis of acetone extracts showed these results (given in mg/L): chlorophyll a 2,386, chlorophyll b 0,332 and carrotenoides 1,037. Data given in mg/g dry substance are: chlorophyll a 1,193x10(-2), chlorophyll b 1,66x10(-3), and carrotenoides 5,185x10(-3). Pigments structure (in mg/L) in species Malva moschata is 1,6 for chlorophyll; 1,419 for chlorophyll b; and 0,364 for carrotenoides. Data given in mg/g are: chlorophyll a 8x10(-3), chlorophyll b 7,09x10(-3), and carrotenoides 1,82x10(-3). Considering that species Malva moschata L. grows on ecologically clear soils as opposed to well-known medicinal species Malva sylvestris L., and considering the production of phytomass, phytochemical structure and pharmacological influence it can be considered very medical and be given advantage over this wider spread category.

  12. Evolutionary and domestication history of Cucurbita (pumpkin and squash) species inferred from 44 nuclear loci.

    Science.gov (United States)

    Kates, Heather R; Soltis, Pamela S; Soltis, Douglas E

    2017-03-10

    Phylogenetics can facilitate the study of plant domestication by resolving sister relationships between crops and their wild relatives, thereby identifying the ancestors of cultivated plants. Previous phylogenetic studies of the six Cucurbita crop lineages (pumpkins and squashes) and their wild relatives suggest histories of deep coalescence that complicate uncovering the genetic origins of the six crop taxa. We investigated the evolution of wild and domesticated Cucurbita using the most comprehensive and robust molecular-based phylogeny for Cucurbita to date based on 44 loci derived from introns of single-copy nuclear genes. We discovered novel relationships among Cucurbita species and recovered the first Cucurbita tree with well-supported resolution within species. Cucurbita comprises a clade of mesophytic annual species that includes all six crop taxa and a grade of xerophytic perennial species that represent the ancestral xerophytic habit of the genus. Based on phylogenetic resolution within-species we hypothesize that the magnitude of domestication bottlenecks varies among Cucurbita crop lineages. Our phylogeny clarifies how wild Cucurbita species are related to the domesticated taxa. We find close relationships between two wild species and crop lineages not previously identified. Expanded geographic sampling of key wild species is needed for improved understanding of the evolution of domesticated Cucurbita.

  13. Purification and characterization of moschins, arginine-glutamate-rich proteins with translation-inhibiting activity from brown pumpkin (Cucurbita moschata) seeds.

    Science.gov (United States)

    Ng, T B; Parkash, A; Tso, W W

    2002-10-01

    From fresh brown pumpkin seeds, two proteins with a molecular mass of 12kDa and an N-terminal sequence rich in arginine and glutamate residues were obtained. The protein designated alpha-moschin closely resembled the fruitfly programmed-cell death gene product and the protein designated beta-moschin demonstrated striking similarity to prepro 2S albumin in N-terminal sequence. alpha- and beta-moschins inhibited translation in the rabbit reticulocyte lysate system with an IC(50) of 17 microM and 300nM, respectively.

  14. In vitro Antioxidant Activity of the Ethanol Extract of Cucurbita moschata D.(pumpkin)%南瓜醇提物的体外抗氧化活性

    Institute of Scientific and Technical Information of China (English)

    李潇霞; 杨建雄

    2008-01-01

    In this study,the antioxidant activities of pumpkin ethanol extract(PEE)were evaluated using various estabscavenging assays,reducing power,β-carotsne/linoleie acid model system,lipid peroxidation.The radical-scavenging effect was in a dose-dependent manner,and the IC50 values for DPPH,superoxide,and hydroxyl radicals were found to be 18.8 mg/mL,29.0 mg/mL and 44.9 μg/mL,respectively.The reducing power and total antioxidant activity were excellent and increasing steadily with increasing amount of sample.In lipid peroxidation assay,the maximum inhibition was 45.81% at 8.26 mg/mL concentration.The results indicated that PEE may be exploited as health food or functional food,to alleviate oxidative stress.%采用化学体系模拟法体外测定南瓜醇提物((pumpkin ethanol extract,PEE)对1,1-二苯基-2-苦苯肼自由基(DPPH·)、超氧阴离子自由基(O2)和羟自由基(·OH)的清除能力,总还原力,对β-胡萝卜素/亚油酸自氧化体系的总抗氧化能力以及脂质过氧化的抑制能力.结果显示PEE对DPPH·、02-和·OH均有较强的清除能力,IC50值分别为18.8 mg/mL、29.0 ms/mL和44.9μg/mL,有显著的还原力和总抗氧化力,对脂质过氧化有一定的抑制作用.PEE的体外抗氧化活性均有良好的量效关系.上述结果为南瓜作为抗氧化的保健食品或功能食品开发利用提供了依据.

  15. Comercialización de semillas importadas de cilantro Coriandrum sativum L. y zapallo Cucurbita moschata durante el periodo enero 1990-agosto 1994

    Directory of Open Access Journals (Sweden)

    Lema Nuñez Rosario M.

    1996-12-01

    Full Text Available

    Of the quantity of imported coriander seeds (3% and of pumpkin (0.2% represent 3.2 % of the total imported a volume of (648.000 kg and 0.79% of the total imported value. Due to a high concentration of these distribution companies in Colombian market (4 or 5 this creates an oligapoly which restricts other producers entering the market, determining the quality, packaging format. The brands are characterized by low prices and reputable quality. The importers fix a high marginal sale (80%, in keeping with the greater percentage (61% of their profits because of low commercialization costs (3% for pumpkin and 5% for coriander. The remaining percentage (14% in coriander and 6% in pumpkin are the detailed marginal output. The preferred variety of pumpkin were Conneticut Field, Candelaria, Sugar, Pie and Golden Delicius, the hybrid seed have not been accepted by the agriculture community.

    Las cantidades importadas de semillas de cilantro (20.393 kg  y de zapallo (1.518 Kg. representaron el 3.2% del volumen total para hortalizas (648.000 kilos y el 0.79% del valor total de dichas importaciones ($8.900 millones. Por la alta concentración del mercado colombiano en 4 ó 5 empresas -productoras y distribuidoras-, constituyen oligopolios, los cuales impiden la entrada de nuevas empresas diferenciando las semillas en forma real (calidad y no real (marca, color del empaque. Las marcas se caracterizan por los precios bajos y calidad. El importador fija márgenes de mercadeo altos (80%, correspondiendo el mayor porcentaje (61% a sus ganancias porque los costos de comercialización son bajos (3% en zapallo y 5% en cilantro. El porcentaje restante (14% en cilantro y 6% en zapallo es el margen del detallista. Las variedades preferidas de zapallo fueron Conneticut Field, Candelaria, Sugar Pie y Golden Delicius; la semilla híbrida no ha sido aceptada por los agricultores. En cilantro se importan el American Long Standing y Coriander (común o de larga duración.

  16. Comercialización de semillas importadas de cilantro coriandrum sativum l. y zapallo cucurbita moschata durante el periodo enero 1990-agosto 1994

    OpenAIRE

    Lema Nuñez, Rosario M.

    2010-01-01

    Las cantidades importadas de semillas de cilantro (20.393 kg)  y de zapallo (1.518 Kg.) representaron el 3.2% del volumen total para hortalizas (648.000 kilos) y el 0.79% del valor total de dichas importaciones ($8.900 millones). Por la alta concentración del mercado colombiano en 4 ó 5 empresas -productoras y distribuidoras-, constituyen oligopolios, los cuales impiden la entrada de nuevas empresas diferenciando las semillas en forma real (calidad) y no real (marca, color del empaque). Las m...

  17. 南瓜和苦瓜的生药鉴定%Pharmacognostic identification of fruit of Cucurbita moschata and Momordica charantia

    Institute of Scientific and Technical Information of China (English)

    刘金荣; 成玉怀; 王航宇; 唐辉

    2001-01-01

    目的为南瓜和苦瓜的鉴定和开发利用提供科学依据.方法显微鉴定和理化鉴定.结果南瓜和苦瓜均有明显的显微和理化鉴别特征.结论这些特征可作为南瓜和苦瓜鉴别的根据.

  18. 南瓜色素提取及稳定性研究%Study on Extraction and Stability of Pigment from Cucurbita Moschata

    Institute of Scientific and Technical Information of China (English)

    张德华; 金明

    2013-01-01

    以南瓜色素为提取产品,研究其提取工艺及产品的稳定条件.结果表明,南瓜色素提取的最佳条件:以无水乙醇为浸提溶剂,料液比为1∶9,浸提温度为60℃,浸提时间为3.0h;每100 g南瓜干品可提取南瓜色素为1.6547 g.研究表明南瓜色素在中性、偏碱性以及较高温度条件下都比较稳定,但对光照比较敏感.

  19. Analysis of Cucurbita Moschata Duch Oil by GC - MS%南瓜籽油脂肪酸的GC-MS分析

    Institute of Scientific and Technical Information of China (English)

    王晓; 程传格; 马小来; 董富英; 刘建华

    2002-01-01

    用正已烷抽提南瓜籽,然后将样品进行皂化、甲酯化,以毛细管色谱-质谱联用法(GC-MS)测定了南瓜籽油的组成,共鉴定出12种成分,其中主要成分为亚油酸和油酸,其相对含量分别为38.60%和28.63%.

  20. Analysis of volatile components from the flesh of chaotianmiben Cucurbita moschata Duch%超甜蜜本南瓜瓤挥发性成分分析

    Institute of Scientific and Technical Information of China (English)

    李昌勤; 张橡楠; 卢引; 康文艺

    2013-01-01

    目的 分析超甜蜜本南瓜瓤挥发性成分.方法 采用顶空固相微萃取和气质联用技术(HS-SPME/GC-MS )结合保留指数法,首次并用峰面积归一化法测定相对百分含量.结果 从超甜蜜本南瓜的瓤中鉴定出31个化合物,占总峰面积的99.28%.结论 研究表明其中棕榈酸乙酯含量最高(22.68%),其次为亚麻酸乙酯(15.57%).

  1. Effects of Different Pruning Methods on Yield of Cucurbita moschata Duch%不同整枝方式对密本南瓜产量影响

    Institute of Scientific and Technical Information of China (English)

    张永刚; 杨选辉; 严立; 余常水

    2014-01-01

    为提高南瓜生产水平,对密本南瓜进行了整枝试验.结果表明:在遵义县试验条件下,整枝留2条子蔓和留3条子蔓能使密本南瓜提前7d采收,单瓜重比不整枝的高30%和31.69%,单产比不整枝的分别高27.56%,29.75%.说明密本南瓜整枝更有利于获得更大的经济价值.

  2. 南瓜矮生性研究的AFLP体系的建立%A Construction of AFLP Analysis System for Dwarf Cucurbita moschata Research

    Institute of Scientific and Technical Information of China (English)

    武峻新; 畅志坚

    2011-01-01

    Based on dwarf and vine inbred lines, key factors of AFLP analysis system( such as genomic DNA extraction, time requirement of digestion and ligation reactions, dilution of pre-amplification and silver staining method et al) were studied. The result showed: the best quality genomic DNA were adding PVP and β-mercaptoethanol modified CTAB method; 5.5 h can be meet digestion and ligation reactions; Diluted 5 times for products of digestion and ligation reactions and diluted 30 times for products of pre-amplification can be meet experimental conditions. 96 primers screened, reproduce, clear polymorphic bands were obtained between dwarf and vine inbred lines. It laid good foundation to tropic pumpkin dwarf vine mapping.%以南瓜矮生和蔓生的自交系为材料,通过对影响南瓜AFLP反应体系中的关键因素(如DNA的提取方法、酶切和连接反应时间、预扩增产物稀释倍数和银染检测方法等)进行研究,结果表明,采用加入PVP和β-巯基乙醇后的改良CTAB提取法获得的南瓜嫩叶基因组DNA质量较好;5.5 h可以满足酶切和连接反应;酶切连接产物稀释5倍,预扩增产物稀释30倍,可以满足试验要求.应用该体系,对96对引物进行筛选,在南瓜矮生和蔓生自交系间获得的引物扩增出了重复性好、清晰的南瓜矮生和蔓生的自交系多态性条带.为进一步进行南瓜矮生基因定位奠定了良好的基础.

  3. 印度南瓜与中国南瓜有性杂交亲和性研究%Study on Affinity of Sexual Hybridization between Cucurbita maxima and Cucurbita moschata

    Institute of Scientific and Technical Information of China (English)

    程永安; 张恩慧; 许忠民; 王妍妮; 付巧玲

    2002-01-01

    为提供用中国南瓜改良印度南瓜的理论依据,选用印度南瓜的3个栽培品种与中国南瓜的4个栽培品种进行人工杂交,观察其杂交亲和性表现.结果表明:印度南瓜P6,P7等品种与中国南瓜P2,P3,P4,P5等品种间存在杂交不亲和性,印度南瓜P1与中国南瓜P4,P5间存在杂交不亲和性,而与P2,P3间不存在杂交不亲和性.

  4. Study Progress on the Formation Mechanism of Hull-less Seed in Cucurbita moschata and Cucurbita pepo%南瓜、西葫芦薄种皮形成机制研究进展

    Institute of Scientific and Technical Information of China (English)

    申琼; 武峻新

    2014-01-01

    南瓜、西葫芦薄种皮由于其特性成为优良的资源材料,近几十年来针对其成因学者们进行了大量研究,主要集中在对薄种皮突变体形成的遗传学机理研究、采用RAPD,AFLP,ISSR,SRAP等标记进行遗传多样性分析、对有皮和薄皮种的种皮组成成分进行分析、对薄皮种进行基因定位等方面.针对目前的研究进行了综述,并对今后有待解决的问题及研究方向进行了展望.

  5. 盐胁迫对黑籽南瓜和白籽南瓜种子萌发特性的影响%Effect of NaCl stress on germination characters of Cucurbita ficifolia and Cucurbita moschata

    Institute of Scientific and Technical Information of China (English)

    王冉; 陈贵林; 梁静; 吕桂云; 卢凤刚

    2005-01-01

    以黑籽南瓜和白籽南瓜种子为材料,用120、180、240 mmol/L NaCl进行处理,对黑籽南瓜和白籽南瓜发芽期的耐盐性进行了比较.结果表明:在各个NaCl浓度下,白籽南瓜的发芽势、发芽率、发芽指数都高于黑籽南瓜,且白籽南瓜种子萌发的活力指数和胚根的根系活力高、胚根细胞质膜渗透率低.比较两者的盐害指数得出,白籽南瓜发芽期耐盐性高于黑籽南瓜.

  6. Preliminary studies of plant distant crossing between Cucurbita moschata Duche and Cucurbita maxima Duche%印度南瓜与中国南瓜的远缘杂交初探

    Institute of Scientific and Technical Information of China (English)

    沈吾山; 许小江; 黄伟忠

    2007-01-01

    以2个中国南瓜为父本、3个印度南瓜为母本杂交,观察其F1代的性状表现,以了解中国南瓜与印度南瓜栽培种间的相互改良与融合的可行性.结果表明,植物学性状中茎、叶、花和抗逆性偏向于父本,具中国南瓜特性;早熟性受父本影响较大;品质中糖类和淀粉受母本的影响较大;父母本果实形状和氨基酸含量都对其产生影响.用中国南瓜和印度南瓜远缘杂交,可产生优良的杂交组合或亲本材料,关键在于父母本的性状表现.

  7. Preliminary Study on Interspecific Transfer of Bush Trait through Cucurbita moschata by Cucurbita maxima%南瓜丛生性状的种间转育研究初报

    Institute of Scientific and Technical Information of China (English)

    刘小俊; 李跃建; 张知仪; 刘独臣; 房超; 梁根云

    2009-01-01

    利用一对种间可杂交材料印度南瓜P1和中国南瓜P2,成功将中国南瓜丛生性状转入印度南瓜.初步分析表明,该丛生性状在印度南瓜中的形态表现和遗传规律与在中国南瓜中一样;它对印度南瓜的蔓生和半丛生性状呈完全显性关系.获得的丛生印度南瓜材料通过进一步研究和改良,可解决生产上存在的印度南瓜早熟、优质,但单产的问题,也可为丛生印度南瓜育种提供一条新途径.

  8. Pollinator and herbivore attraction to cucurbita floral volatiles.

    Science.gov (United States)

    Andrews, Elizabeth S; Theis, Nina; Adler, Lynn S

    2007-09-01

    Mutualists and antagonists may place conflicting selection pressures on plant traits. For example, the evolution of floral traits is typically studied in the context of attracting pollinators, but traits may incur fitness costs if they are also attractive to antagonists. Striped cucumber beetles (Acalymma vittatum) feed on cucurbits and are attracted to several volatiles emitted by Cucurbita blossoms. However, the effect of these volatiles on pollinator attraction is unknown. Our goal was to determine whether pollinators were attracted to the same or different floral volatiles as herbivorous cucumber beetles. We tested three volatiles previously found to attract cucumber beetles in a factorial design to determine attraction of squash bees (Peponapis pruinosa), the specialist pollinators of cucurbita species, as well as the specialist herbivore A. vittatum. We found that 1,2,4-trimethoxybenzene was attractive to both the pollinator and the herbivore, indole was attractive only to the herbivore, and (E)-cinnamaldehyde was attractive only to the pollinator. There were no interactions among volatiles on attraction of squash bees or cucumber beetles. Our results suggest that reduced indole emission could benefit plants by reducing herbivore attraction without loss of pollination, and that 1,2,4-trimethoxybenzene might be under conflicting selection pressure from mutualists and antagonists. By examining the attraction of both mutualists and antagonists to Cucurbita floral volatiles, we have demonstrated the potential for some compounds to influence only one type of interaction, while others may affect both interactions and possibly result in tradeoffs. These results shed light on the potential evolution of fragrance in native Cucurbita, and may have consequences for yield in agricultural settings.

  9. Distribution and abundance of Eledone cirrhosa (Lamarck, 1798 and Eledone moschata (Lamarck, 1798 (Cephalopoda: Octopoda in the Mediterranean Sea

    Directory of Open Access Journals (Sweden)

    Paola Belcari

    2002-06-01

    Full Text Available Information on distribution, abundance and size composition of the two octopods Eledone cirrhosa and E. moschata was obtained from the MEDITS trawl surveys, carried out in a wide area of the Mediterranean basin from 1994 to 1999. Both species showed a wide geographic distribution, as they were collected in all the major areas investigated. E. cirrhosa showed a wide depth distribution, down to the 800 m isobath, while E. moschata was mostly restricted to within 200 m. Further analysis on spatio-temporal basis with a Generalised Linear Model, evidenced that differences among major areas, depth strata and their interaction were always significant. In the case of E. cirrhosa, differences among years and year-major area interaction were also significant. Two cohorts can be singled out in the size frequency distributions of E. cirrhosa, whereas only one mode can be clearly distinguished in most of the length distributions of E. moschata.

  10. Annotated world bibliography of host plants of the melon fly, Bactrocera cucurbitae(Cocquillett)(Diptera:Tephritidae)

    Science.gov (United States)

    The melon fly, Bactrocera cucurbitae(Coquillett), is a widespread, economically important tephritid fruit fly (Diptera: Tephritidae) species. Bactrocera cucurbitae infests fruits and vegetables of a number of different plant species, with many host plants in the plant family Cucurbitaceae, but with ...

  11. Molecular cloning and expression of a bush related CmV1 gene in tropical pumpkin.

    Science.gov (United States)

    Wu, Tao; Cao, Jiashu

    2010-02-01

    A bush-type plant was selected from tropical pumpkin 'cga' (Cucurbita moschata Duchesne) in order to study the vine development in C. moschata. In this study, a novel gene encoding NADH dehydrogenase was isolated from the vine line (cgaV) of C. moschata, that was not expressed in the near isogenic bush line (cgaBu). This gene, designated as CmV1 (C. moschata vine 1), was 545 bp in length and was composed of a 477 bp open reading frame, which had 99% nucleotide similarity to the chloroplast ndhJ gene for NADH dehydrogenase subunit J from Brassica oleracea. The deduced amino acid sequence of CmV1 had 99% similarity to NADH dehydrogenase subunit J from Arabidopsis and had 98% similarity to NADH dehydrogenase subunit from Barbarea verna. Analysis of the basic characteristics of the CmV1 protein revealed that it has one Respiratory chain NADH dehydrogenase 30 kD subunit signature, three N-myristoylation sites, one Casein kinase II phosphorylation site, and one Protein kinase C phosphorylation site. Reverse transcriptase-PCR analysis showed that CmV1 was expressed at a high level in the internodes and hypocotyls and was expressed stronger in elongating internodes than in fully expanded internodes. In conclusion, results obtained in the present study suggest that CmV1 gene might play important roles in vine elongation of tropical pumpkin.

  12. Effect of roasting on tocopherols of gourd seeds (Cucurbita pepo

    Directory of Open Access Journals (Sweden)

    Didier, Montet

    2006-12-01

    Full Text Available The effect of roasting at high temperatures on the Vitamin E in hulled gourd seeds of Cucurbita pepo was studied. Roasting at 140°C for 5 min induced a swelling of the seed with an increase in volume of 43 %, and a weight loss of 6.5 %.The seed acquired the desired puffed-up appearance. The water activity of raw seeds was 0.544 and decreased during roasting to 0.105. Cucurbita pepo seeds contained 51.0 % fat. Tocopherol content of roasted seeds was 68mg/100g oil and of non roasted seeds was 107mg/100g oil. γ-tocopherol represented 96% of the total tocopherols. Total tocopherol loss during roasting was 36%. β-tocopherol loss was the highest at 50%; for α-tocopherol it was 41% and for γ-tocopherol it was 36%.Se ha estudiado el efecto del tostado a alta temperatura sobre la vitamina E en semillas de calabaza Cucurbita pepo. El tostado a 140°C durante 5 min indujo una hinchazón de la semilla con un aumento en el volumen del 43 %, y una pérdida de peso del 6,5 %. La semilla adquirió la apariencia deseada después del tostado. La actividad del agua en la semilla cruda fue de 0,544 y disminuyó durante el tostado a 0,105. Las semillas de Cucurbita pepo contenían un 51,0 % de grasa. El contenido de tocoferol de las semillas tostadas fue de 68mg/100g y el de las semillas no tostadas de 107mg/100g de aceite. El γ-tocoferol representó el 96% de los tocoferoles totales. La pérdida de tocoferoles totales durante el tostado fue del 36% siendo la má alta la del β-tocoferol con un 50%; la del α-tocoferol fue del 41% y la del γ-tocoferol del 36%.

  13. High-throughput SNP genotyping in Cucurbita pepo for map construction and quantitative trait loci mapping

    OpenAIRE

    2012-01-01

    Background: Cucurbita pepo is amember of the Cucurbitaceae family, the second-most important horticultural family in terms of economic importance after Solanaceae. The ¿summer squash¿ types, including Zucchini and Scallop, rank among the highest-valued vegetables worldwide. There are few genomic tools available for this species. The first Cucurbita transcriptome, along with a large collection of Single Nucleotide Polymorphisms (SNP), was recently generated using massive sequencing. A set o...

  14. Lycopene and flesh colour differences in grafted and non-grafted watermelon

    Directory of Open Access Journals (Sweden)

    Fekete D.

    2015-01-01

    Full Text Available The experiment was carried out in three regions in Hungary (Jászszentandrás, Cece, Újkígyós in 2013 to determine the fruit quality of grafted watermelon (Citrullus lanatus Thunb.. The “RX 467” seedless watermelon variety was grafted on two commercial rootstocks “FR STRONG” [Lagenaria siceraria (Mol. Standl.] and “RS 841” (Cucurbita maxima Duchesne × Cucurbita moschata Duchesne. The lycopene and flesh colours are important quality characteristics even of the selfrooted and grafted watermelon. Some differences can be attributed to different environments, technological methods and to the type of rootstockscion combination. Lycopene is a strong antioxidant; therefore, we considered to examine the content change. Regardless of growing location, the lycopene concentration and the chroma (C* showed the best result in the case of interspecific rootstock. The result also showed that in two regions (Jászszentandrás, Cece we can find negative correlation between the lycopene concentration and the L* value of the flesh colour.

  15. Three new triterpene esters from pumpkin (Cucurbita maxima) seeds.

    Science.gov (United States)

    Kikuchi, Takashi; Ueda, Shinsuke; Kanazawa, Jokaku; Naoe, Hiroki; Yamada, Takeshi; Tanaka, Reiko

    2014-04-16

    Three new multiflorane-type triterpene esters, i.e. 7α-hydroxymultiflor-8-ene-3α,29-diol 3-acetate-29-benzoate (1), 7α-methoxymultiflor-8-ene-3α,29-diol 3,29-dibenzoate (2), and 7β-methoxymultiflor-8-ene-3α,29-diol 3,29-dibenzoate (3), were isolated from seeds of Cucurbita maxima, along with the known compound, multiflora-7,9(11)-diene-3α,29-diol 3,29-dibenzoate (4). Compound 1 exhibited melanogenesis inhibitory activities comparable with those of arbutin. In cytotoxicity assays, compounds 1 and 3 exhibited weak cytotoxicity, with IC50 values of 34.5-93.7 μM against HL-60 and P388 cells.

  16. Electrochemical and Corrosion Inhibition Studies of Cucurbita Maxima

    Directory of Open Access Journals (Sweden)

    K. Anbarasi

    2016-08-01

    Full Text Available The influence of the acid extract of peel of Cucurbita maxima (PCM on the corrosion of mild steel in 1N H2SO4 was investigated by weight loss, polarization and impedance methods and SEM analysis. The inhibition efficiency increases with extract concentration and immersion period. Weight loss and corrosion rates of mild steel decreased as the concentration of inhibitor increased. The results showed that PCM was potential corrosion inhibitor and maximum inhibition efficiency (IE % obtained was 98% for 3%PCM at 1h. Impedance measurement results an increase in charge transfer resistance (Rct, which also confirms the corrosion inhibitive nature of the plant extract. Potentiodynamic study showed that PCM acts as a mixed type of inhibitor, which controls both the anodic and cathodic reactions. Scanning electron microscopic studies provided the evidence of improved surface condition for the corrosion protection, due to the adsorption.

  17. Earliest Cucurbita from the Great Lakes, Northern USA

    Science.gov (United States)

    Monaghan, G. William; Lovis, William A.; Egan-Bruhy, Kathryn C.

    2006-03-01

    Directly dated Cucurbita from archaeological sites near Lake Huron expand the range and human usage of adventive, cultivated wild gourds or squash into the Great Lakes region, USA, by 4000 14C yr BP. The data also show that domesticated C. pepo squash was cultivated there by 3000 14C yr BP. Although milder Hypsithermal climate may have been a contributing factor, squash and gourds expanded northward during the mid-Holocene mainly by human agency and may be the first human-introduced adventive plant in temperate North America. Even after 3000 14C yr BP, when domesticated squash generally replaced wild varieties at northern sites, squash stands were probably informally managed rather than intensively cultivated.

  18. Genes for "Reverse" fruit striping in squash (Cucurbita pepo).

    Science.gov (United States)

    Paris, Harry S

    2009-01-01

    Longitudinal striping is a common fruit color pattern in Cucurbita pepo and is conferred by any of several alleles at the light coloration-1 locus. Normally, dark stripes appear over the fruit surface areas between the 10 main subepidermal vein tracts with the lighter background color over and adjacent to these vein tracts. Less commonly, the striping is "reversed," that is, lighter than the background color. The present work was conducted to elucidate the mode of inheritance of reverse striping. The results indicated that reverse striping is conferred by the complementary interaction of an allele for striping with a heretofore unidentified allele at the light coloration-2 locus which is hereby designated l-2(R). Fruits of plants carrying an allele for striping and that are homozygous for l-2(R) have completely reversed striping, whereas those heterozygous for l-2(R) have striping that is completely normal or that is partially normal and partially reversed.

  19. Gourds and squashes (Cucurbita spp.) adapted to megafaunal extinction and ecological anachronism through domestication.

    Science.gov (United States)

    Kistler, Logan; Newsom, Lee A; Ryan, Timothy M; Clarke, Andrew C; Smith, Bruce D; Perry, George H

    2015-12-01

    The genus Cucurbita (squashes, pumpkins, gourds) contains numerous domesticated lineages with ancient New World origins. It was broadly distributed in the past but has declined to the point that several of the crops' progenitor species are scarce or unknown in the wild. We hypothesize that Holocene ecological shifts and megafaunal extinctions severely impacted wild Cucurbita, whereas their domestic counterparts adapted to changing conditions via symbiosis with human cultivators. First, we used high-throughput sequencing to analyze complete plastid genomes of 91 total Cucurbita samples, comprising ancient (n = 19), modern wild (n = 30), and modern domestic (n = 42) taxa. This analysis demonstrates independent domestication in eastern North America, evidence of a previously unknown pathway to domestication in northeastern Mexico, and broad archaeological distributions of taxa currently unknown in the wild. Further, sequence similarity between distant wild populations suggests recent fragmentation. Collectively, these results point to wild-type declines coinciding with widespread domestication. Second, we hypothesize that the disappearance of large herbivores struck a critical ecological blow against wild Cucurbita, and we take initial steps to consider this hypothesis through cross-mammal analyses of bitter taste receptor gene repertoires. Directly, megafauna consumed Cucurbita fruits and dispersed their seeds; wild Cucurbita were likely left without mutualistic dispersal partners in the Holocene because they are unpalatable to smaller surviving mammals with more bitter taste receptor genes. Indirectly, megafauna maintained mosaic-like landscapes ideal for Cucurbita, and vegetative changes following the megafaunal extinctions likely crowded out their disturbed-ground niche. Thus, anthropogenic landscapes provided favorable growth habitats and willing dispersal partners in the wake of ecological upheaval.

  20. Calcium Distribution in Globoid Crystals of Cucurbita Cotyledon Protein Bodies 1

    Science.gov (United States)

    Lott, John N. A.; Spitzer, Ernest; Vollmer, Catherine M.

    1979-01-01

    Energy-dispersive x-ray analysis was used to investigate the location of globoid crystals with relatively high Ca levels within cotyledons of Cucurbita maxima, Cucurbita mixta, and Cucurbita andreana. The small globoid crystals in both upper and lower epidermal cells commonly contained Ca. Ca was present in globoid crystals of all provascular regions with the exception of the very small provascular regions of C. maxima. In C. maxima and C. mixta cotyledons, some cases were observed where Ca was found in the globoid crystals of the first layer of mesophyll cells surrounding the provascular region, but in general Ca was absent from globoid crystals of palisade and spongy mesophyll cells. In C. andreana, globoid crystals of palisade and spongy mesophyll cells commonly contained at least some Ca. Cell position and cell type are factors affecting the Ca content of globoid crystals in protein bodies. PMID:16660825

  1. Sensory Acceptability of Squash (Cucurbita Maximain Making Ice Cream

    Directory of Open Access Journals (Sweden)

    Raymund B. Moreno

    2015-02-01

    Full Text Available - This experimental research was conducted to determine the sensory acceptability of mashed squash (Cucurbita Maxima of different proportions in making ice cream in terms of appearance, aroma, texture, taste and general acceptability. Five treatments were formulated in the study—four of which utilized mashed squash at various proportions and one treatment was used as the control variable which contains no mashed squash at all. The respondents of the study were the 20 Food Technology students and 10 faculty members of West Visayas State University Calinog Campus who were selected through random sampling. The respondents evaluated the finished products using a modified sensory evaluation score sheet based on Six Point Hedonic Scale. The statistical tools used were the means, standard deviation, Wilcoxon Signed Rank Test. The 0.01 alpha level was used as the criterion for acceptance or rejection of the null hypothesis. The result of the study led to the conclusion that there is a significant difference that existed in the level of acceptability of mashed squash in making ice cream in terms of appearance, aroma, and general acceptability, therefore the null hypothesis is rejected. However, no significant difference in the level of acceptability of using mashed squash in making ice cream in terms of taste and texture.

  2. Identification of viruses infecting pumpkins (Cucurbita pepo L in Yugoslavia

    Directory of Open Access Journals (Sweden)

    Krstić Branka B.

    2002-01-01

    Full Text Available This study was carried out in order to identify the major viruses infecting pumpkins (Cucurbita pepogrown in Serbia. Leaf samples from virus-infected pumpkin plants were collected in mid-July 2001. Naked-seeded and hulled oil pumpkins, patty pan, zucchini and summer squash from three different locations were included (Table 1. Virus-infected plants showed different symptoms (Table 2 and Figures 1-4. Due to the great variability of the symptoms, the causal viruses could not be fully and precisely determined by visual examination only. The infected samples were tested by the biotest, as well as by two serological methods, ELISA and EBIA. Polyclonal antibodies raised against cucumber mosaic cucumovirus (CMV, zucchini yellow mosaic potyvirus (ZYMV, watermelon mosaic potyvirus 1 (WMV-1, watermelon mosaic potyvirus 2 (WMV-2 and squash mosaic comovirus (SqMV were used. In each of the 50 collected samples one or two viruses were detected (Tables 3 and 4. The most prevalent viruses infecting pumpkins were ZYMV (62% and CMV (58%. WMV-2 was extremely rare.

  3. Shoot production in squash (Cucurbita pepo) by in vitro organogenesis.

    Science.gov (United States)

    Ananthakrishnan, G; Xia, X; Elman, C; Singer, S; Paris, H S; Gal-On, A; Gaba, V

    2003-04-01

    Seedling-derived cotyledon explants of squash ( Cucurbita pepo L.) of commercial cultivars True French, Ma'yan and Goldy were regenerated in vitro on Murashige and Skoog medium augmented with 1 mg/l benzyladenine. After 4 weeks in culture small shoots and buds regenerated only on the most proximal cotyledon edge. Culture on an elongation medium with a reduced cytokinin concentration (0.1 mg/l) with or without 1 mg/l gibberellic acid (GA(3)) facilitated the recovery of shoots. Fresh shoots could be recovered at each subculture of the regenerating mass. Peak productivity was during the third cycle of subculture, and shoot production ceased after the fifth subculture. Culture on elongation medium supplemented with GA(3) was 55% more effective with respect to overall shoot production than that on medium without GA(3), with 22 shoots recovered in total per explant from the former. Regeneration occurred under both light and dark conditions. All of the shoots tested were diploid. The shoots were rooted and transferred to the greenhouse where they grew and flowered normally.

  4. Inheritance of parthenocarpy in summer squash (Cucurbita pepo L.).

    Science.gov (United States)

    Menezes, Cícero B de; Maluf, Wilson R; Azevedo, Sebastião M de; Faria, Marcos V; Nascimento, Ildon R; Nogueira, Douglas W; Gomes, Luiz A A; Bearzoti, Eduardo

    2005-03-31

    The inheritance of the tendency to set parthenocarpic fruit in the summer squash (Cucurbita pepo L.) line Whitaker was studied. Two parental lines, Whitaker (parthenocarpic) and Caserta (non-parthenocarpic), and the F1 and F2 generations and backcrosses to both parents were tested. The parthenocarpic tendency of individual plants was scored on a scale from 1 (non-parthenocarpic fruit) to 5 (parthenocarpic fruit). The Whitaker line produced parthenocarpic fruit and had a mean score of 4.2, whereas Caserta did not set parthenocarpic fruit and had a score of 1.55. The heritability estimates indicated that genetic gains from selection were feasible. The additive-dominant model showed a good fit, with epistasis being negligible or nonexistent. The hypothesis of monogenic inheritance with incomplete dominance was not rejected within the degree of dominance range from 0.2 to 0.5. These results indicate that parthenocarpy is controlled by a single locus, with incomplete dominance in the direction of parthenocarpic expression.

  5. Chemical Profile, Antioxidant and Antibacterial Activities of Achillea moschata Wulfen, an Endemic Species from the Alps

    Directory of Open Access Journals (Sweden)

    Sara Vitalini

    2016-06-01

    Full Text Available Aerial parts of Achillea moschata Wulfen (Asteraceae growing wild in the Italian Rhaetian Alps were investigated to describe, for the first time, their phenolic content, as well as to characterize the essential oil. Inspection of the metabolic profile combining HPLC-DAD and ESI-MS/MS data showed that the methanol extract contained glycosylated flavonoids with luteolin and apigenin as the main aglycones. Among them, the major compound was 7-O-glucosyl apigenin. Caffeoyl derivates were other phenolics identified. The essential oil obtained by steam distillation and investigated by GC/FID and GC/MS showed camphor, 1,8-cineole, and bornylacetate as the main constituents. The antioxidant capacity of three different extracts with increasing polarity and of the essential oil was evaluated by employing ABTS·+ and DPPH· radical scavenging assays. The methanolic extract was the only significantly effective sample against both synthetic radicals. All samples were also tested against Gram-positive (Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus and Gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa bacterial species using the disk diffusion assay. The non-polar extracts (dichloromethane and petroleum ether and the essential oil possessed a broad spectrum of antimicrobial activity expressed according to inhibition zone diameter (8–24 mm.

  6. GH gene polymorphisms and expression associated with egg laying in muscovy ducks (Cairina moschata).

    Science.gov (United States)

    Wu, X; Yan, M J; Lian, S Y; Liu, X T; Li, A

    2014-02-01

    Accumulated evidence suggests that the growth hormone (GH) gene plays a physiological role in the control of reproductive function. Here, we examined the correlation between egg-laying traits and GH gene polymorphisms and expression patterns in the muscovy duck (Cairina moschata). PCR single-strand conformation polymorphism was used to identify polymorphisms in intron 3 of GH. One single nucleotide polymorphism (g.3270 A > G) was detected by sequencing, and the frequencies of the A and G alleles in the population were 0.65 and 0.35, respectively. A comparison test showed that the AA genotype group had more consecutive laying days and more eggs at 300 days than the GG genotype group (P 0.05). Such a significant correlation between GH polymorphisms and egg-laying performance suggested that GH could be a candidate locus affecting the laying trait in muscovy duck. Furthermore, real-time fluorescent quantitative PCR demonstrated that GH is expressed in all selected tissues, but is highly expressed in the hypothalamic-pituitary-gonadal axis and heart. This unique expression pattern suggested that GH may exert its local physiological function through the autocrine or paracrine pathway during gonad development and growth in the muscovy duck. The data presented in this paper revealed GH polymorphisms and expression patterns in the muscovy duck and indicated a potential regulatory effect of GH on reproduction.

  7. Laboratory evaluation of the chemosterilant lufenuron against Ceratitis capitata, Bactrocera dorsalis, B. cucurbitae, and B. latifrons.

    Science.gov (United States)

    Four species of tephritid fruit flies, Ceratitis capitata, Bactrocera dorsalis, B. cucurbitae, and B. latifrons were evaluated for toxic, developmental, and physiological responses to the chemosterilant lufenuorn incorporated in an agar adult diet and a liquid larval diet. No significant mortality o...

  8. WATERMELON MOSAIC VIRUS OF PUMPKIN (Cucurbita maxima FROM SULAWESI: IDENTIFICATION, TRANSMISSION, AND HOST RANGE

    Directory of Open Access Journals (Sweden)

    Wasmo Wakmana

    2016-10-01

    Full Text Available A mosaic disease of pumpkin (Cucurbita maxima was spread widely in Sulawesi. Since the virus had not yet been identified, a study was conducted to identify the disease through mechanical inoculation, aphid vector transmission, host range, and electron microscopic test. Crude sap of infected pumpkin leaf samples was rubbed on the cotyledons of healthy pumpkin seedlings for mechanical inoculation. For insect transmission, five infective aphids were infected per seedling. Seedlings of eleven different species were inoculated mechanically for host range test. Clarified sap was examined under the electron microscope. Seeds of two pumpkin fruits from two different infected plants were planted and observed for disease transmission up to one-month old seedlings. The mosaic disease was transmitted mechanically from crude sap of different leaf samples to healthy pumpkin seedlings showing mosaic symptoms. The virus also infected eight cucurbits, i.e., cucumber (Cucumis sativus, green melon (Cucumis melo, orange/rock melon (C. melo, zucchini (Cucurbita pepo, pumpkin (Cucurbita maxima, water melon (Citrulus vulgaris, Bennicosa hispida, and blewah (Cucurbita sp.. Aphids  transmitted the disease from one to other pumpkin seedlings. The virus was not transmitted by seed. The mosaic disease of pumpkin at Maros, South Sulawesi, was associated with flexious particles of approximately 750 nm length, possibly a potyvirus, such as water melon mosaic virus rather than papaya ringspot virus or zucchini yellow mosaic virus.

  9. Estimation of humoral immune response in rabbits fed with Cucurbita maxima seeds

    Directory of Open Access Journals (Sweden)

    V. Ranganathan

    Full Text Available Aim : The objective of the study was to estimate the humoral immune response in rabbits treated with Cucurbita maxima seeds. Materials and Methods: Thirty six male Newzealand White rabbits were divided into six groups (I, II, III, IV, V, VI of six in each. Group I was the untreated control. Group II was treated with dexamethasone sodium (2 mg/Kg, i.m for 7 days. Group III was treated with levamisole hydrochloride at 2.5 mg/kg (s.c thrice a week. Group IV was treated with Cucurbita maxima seeds. Group V was treated with levamisole and dexamethasone and Group VI was treated with dexamethasone and Cucurbita maxima seeds. The seed was given @ 1000 mg/kg orally for 10 days. Antibody titre and serum immunoglobulin concentration were estimated along with haematology. Results: Dexamethasone caused significant decreases in the antibody titre, immunoglobulin concentration where as Curcurbita maxima, Dexamethasone + Curcurbita maxima and dexamethasone + levamisole groups showed significant increase in these entities. There were no significant differences in RBC count, Haemoglobin contents among all the groups studied. Conclusion: Results suggest that Cucurbita maxima seeds has the ability to stimulate humoral immune response in rabbits. [Vet World 2013; 6(7.000: 396-399

  10. Relative incidence of Bactrocera cucurbitae (Coquillett) and Dacus ciliatus Loew on cucurbitaceous vegetables

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, N.K. Krishna; Verghese, Abraham; Shivakumara, B.; Krishnamoorthy, P.N.; Ranganath, H.R. [Indian Institute of Horticultural Research, Bangalore (India). Div. of Entomology and Nematology

    2006-07-01

    The melon fly, Bactrocera cucurbitae (Coquillett) is a major pest of cucurbitaceous vegetables and fruits in many parts of the world. Infestation of an another species, the lesser pumpkin fly, Dacus ciliatus Loew is reported on a few cucurbits in the Indian sub-continent and Africa. While extensive work on seasonality, infestation percent, host preference, attraction to para pheromone on B. cucurbitae has been reported, little is known of D. ciliatus. Field experiments were carried out at the Indian Institute of Horticultural Research (IIHR), Bangalore (12058'N; 77035'E) from June 2002- October 2003. Cucumber (Cucumis sativus L), ridge gourd (Luffa acutangula (L.) Roxb), bitter gourd (Momordica charantia L.) and pickling cucumbers [C. sativus L (variety. Ijax)] were raised at monthly interval. Cue lure baited bottle traps were hung to monitor B. cucurbitae and other related species. Bactrocera cucurbitae was present all through the year and maximum number of adults was trapped during August (14.14/trap/week). Dacus ciliatus was trapped only from May to October but in relatively less numbers ({approx} 1/week). Maximum fruit fly infestation was 77.03 % on bitter gourd (August 2003), 75.65 % on ridge gourd (Nov. 02), 73.83 % on cucumber (October, 02) and 63.31 % on pickling cucumber (October, 02). Trap catches of B. cucurbitae was significantly and positively correlated with relative humidity. Maximum and minimum temperature, RH (%), rainfall (mm), evaporation (mm) and wind speed (km/h) collectively determined 44 % of B. cucurbitae trap catches. Maximum fruit fly emergence of 494.64/ kg fruit was on bitter gourd (October, 2002) followed by cucumber (431.97, November, 2002), pickling cucumber (307.51, October 2002) and ridge gourd (210.74, October, 2003). Dacus ciliatus formed only 4.5% of the total number of fruit flies on bitter gourd and 0.2% on pickling cucumber. Its infestation was not observed on cucumber and ridge gourd. Parasitism by the larval

  11. High-throughput SNP genotyping in Cucurbita pepo for map construction and quantitative trait loci mapping

    Directory of Open Access Journals (Sweden)

    Esteras Cristina

    2012-02-01

    Full Text Available Abstract Background Cucurbita pepo is a member of the Cucurbitaceae family, the second- most important horticultural family in terms of economic importance after Solanaceae. The "summer squash" types, including Zucchini and Scallop, rank among the highest-valued vegetables worldwide. There are few genomic tools available for this species. The first Cucurbita transcriptome, along with a large collection of Single Nucleotide Polymorphisms (SNP, was recently generated using massive sequencing. A set of 384 SNP was selected to generate an Illumina GoldenGate assay in order to construct the first SNP-based genetic map of Cucurbita and map quantitative trait loci (QTL. Results We herein present the construction of the first SNP-based genetic map of Cucurbita pepo using a population derived from the cross of two varieties with contrasting phenotypes, representing the main cultivar groups of the species' two subspecies: Zucchini (subsp. pepo × Scallop (subsp. ovifera. The mapping population was genotyped with 384 SNP, a set of selected EST-SNP identified in silico after massive sequencing of the transcriptomes of both parents, using the Illumina GoldenGate platform. The global success rate of the assay was higher than 85%. In total, 304 SNP were mapped, along with 11 SSR from a previous map, giving a map density of 5.56 cM/marker. This map was used to infer syntenic relationships between C. pepo and cucumber and to successfully map QTL that control plant, flowering and fruit traits that are of benefit to squash breeding. The QTL effects were validated in backcross populations. Conclusion Our results show that massive sequencing in different genotypes is an excellent tool for SNP discovery, and that the Illumina GoldenGate platform can be successfully applied to constructing genetic maps and performing QTL analysis in Cucurbita. This is the first SNP-based genetic map in the Cucurbita genus and is an invaluable new tool for biological research

  12. Pumpkin Seed Oil Extracted From Cucurbita maxima Improves Urinary Disorder in Human Overactive Bladder.

    Science.gov (United States)

    Nishimura, Mie; Ohkawara, Tatsuya; Sato, Hiroji; Takeda, Hiroshi; Nishihira, Jun

    2014-01-01

    The pumpkin seed oil obtained from Cucurbita pepo has been shown to be useful for the treatment of nocturia in patients with urinal disorders in several western countries. In this study, we evaluated the effect of the pumpkin seed oil from Cucurbita maxima on urinary dysfunction in human overactive bladder (OAB). Forty-five subjects were enrolled in this study. An extract of pumpkin seed oil from C. maxima (10 g of oil/day) was orally administrated for 12 weeks. After 6 and 12 weeks, urinary function was evaluated using Overactive Bladder Symptom Score (OABSS). Pumpkin seed oil from C. maxima significantly reduced the degree of OABSS in the subjects. The results from our study suggest that pumpkin seed oil extracts from C. maxima as well as from C. pepo are effective for urinary disorders such as OAB in humans.

  13. Flowering biology, nectar production and insect visits in Cucurbita pepo L. flowers

    Directory of Open Access Journals (Sweden)

    Marta Dmitruk

    2012-12-01

    Full Text Available In 1998-2000 studies on length and abundance of flowering and on nectar productivity of zucchini and marrow (Cucurbita pepo L. were carried out in Lublin area. Flowers visitors were also monitored. Flowering of plants lasted from the end of June till the end of September. The mean number of flowers per plant of zucchini reached: 31 (male flowers and 26 (female flowers, and for marrow 226 and 22, respectively. Flowers lived, on average, for 5 hours. Female flowers of marrow secreted the highest amount of nectar - 1.354 g per 10 flowers, on average. Sugar content in nectar was 21.84%-27.31%. The mean total amount of sugars secreted by 10 flowers of Cucurbita pepo L. was 21.5-304.3 mg. Pollinators were mainly bumblebees and honey bees.

  14. Chloroplast phylogeny of Cucurbita: Evolution of the domesticated and wild species

    Institute of Scientific and Technical Information of China (English)

    Yi-Hong ZHENG; Andrew J.ALVERSON; Qing-Feng WANG; Jeffrey D.PALMER

    2013-01-01

    The genus Cucurbita (Cucurbitaceae) includes five species that were domesticated independently in the Americas,giving rise to an immense diversity of squashes,pumpkins,and gourds.To gain an improved understanding of the evolution of Cucurbita and its domesticated taxa,we used four chloroplast loci to estimate the phylogeny of 23 taxa that represent the broad-level diversity within Cucurbita.Our results provide a strongly supported framework hypothesis for the phylogeny of the genus,robustly confirming the basal position of the C.digitata group of xerophytic perennials and the monophyly of a large group of mesophytic annuals that represent most of the known diversity in the genus,both wild and domesticated.The chloroplast evidence provides strong support for a novel grouping of the mesophytic annual C ficifolia (known only from cultivation) with the xerophytic perennials C.foetidissima and C.pedatifolia.This study also provides the first DNA-based evidence in support of the isozymebased hypothesis that C.pepo subsp.ovifera var.ovifera (represented by most ornamental gourds and several squashes) was domesticated from the wild taxon C.pepo subsp.ovifera var.ozarkana.This lends support to the hypothesis that var.ovifera was domesticated in the eastern United States and that this region served as one of about 10 independent centers of origin of human agriculture.Although the level of bootstrap support for this and certain other peripheral relationships in Cucurbita is low,definitive resolution of these issues is within reach,as nextgeneration sequencing should soon deliver entire organelle genome sequences from a comprehensive sampling of the genus.

  15. Chemotaxonomical study of vegetables X. The carotenoids of some vegetables from Cucurbita genus

    Directory of Open Access Journals (Sweden)

    Gavrila NEAMTU

    1978-08-01

    Full Text Available Im Rahmen unserer chemotaxonomischen Untersuchungen uber die Carotinoide hoherer Pflanzen (1, 2, 3, 4, 5 befassen wir uns in vorliegender Arbeit mit den Carotinoiden einiger Pflanzen der Gattung Cucurbita. Wir berichten von C. maxima Duch. var. bananina Greb., C. maxima Duch. var. maxima cv. Marica, C. pepo var. oblonga Greb. und C. pepo var. pyriformis Alef., unter Hinweis auf carotenotaxonomisch interessante Befunde.

  16. Isolation and Molecular Characterization of the Transformer Gene From Bactrocera cucurbitae (Diptera: Tephritidae)

    Science.gov (United States)

    Luo, Ya; Zhao, Santao; Li, Jiahui; Li, Peizheng

    2017-01-01

    transformer (tra) is a switch gene of sex determination in many insects, particularly in Dipterans. However, the sex determination pathway in Bactrocera cucurbitae (Coquillett), a very destructive pest on earth, remains largely uncharacterized. In this study, we have isolated and characterized one female-specific and two male-specific transcripts of the tra gene (Bcutra) of B. cucurbitae. The genomic structure of Bcutra has been determined and the presence of multiple conserved Transformer (TRA)/TRA-2 binding sites in Bcutra has been found. BcuTRA is highly conservative with its homologues in other tephritid fruit flies. Gene expression analysis of Bcutra at different developmental stages demonstrates that the female transcript of Bcutra appears earlier than the male counterparts, indicating that the maternal TRA is inherited in eggs and might play a role in the regulation of TRA expression. The conservation of protein sequence and sex-specific splicing of Bcutra and its expression patterns during development suggest that Bcutra is probably the master gene of sex determination of B. cucurbitae. Isolation of Bcutra will facilitate the development of a genetic sexing strain for its biological control.

  17. COMPARATIVE STUDIES ON ANTHELMINTIC POTENTIAL OF CUCURBITA MAXIMA (PUMPKIN SEEDS AND CARICA PAPAYA (PAPAYA SEEDS

    Directory of Open Access Journals (Sweden)

    Sengupta Rupa

    2013-08-01

    Full Text Available The crude extract of Carica papaya (papaya seeds (CP and Cucurbita maxima (Pumpkin seeds (CM were assayed against adult earthworms (Pheretima posthuma for the evaluation of anthelmintic activity. Various concentrations of both extracts were tested and results were expressed in terms of time for paralysis (P and time for death (D of worms. Albendazole was used as a reference standard. The result showed that in both of the extracts (i.e. CP and CM dose of 60 mg / ml possesses more wormicidal activity. The time of paralysis was 1.88 ± 0.52 minute and 1.93 ± 0.57 minute whereas the time of death was 3.45 ± 0.17 minute and 4.90 ± 0.18 minute in the case of Carica papaya and Cucurbita maxima respectively. In conclusion, the use of seeds of Carica papaya (CP and Cucurbita maxima (CM for anthelmintic activity have been confirmed and further studies are suggested to isolate the active principles responsible for the activity. Both the extracts showed significant anthelmintic activity, but the comparative study showed that out of these two, Carica papaya proves to be a better anthelmintic remedy.

  18. Purification of Colocasia esculenta lectin and determination of its anti-insect potential towards Bactrocera cucurbitae.

    Science.gov (United States)

    Thakur, Kshema; Kaur, Manpreet; Kaur, Satwinder; Kaur, Amritpal; Kamboj, Sukhdev Singh; Singh, Jatinder

    2013-01-01

    The present study reports the purification of a lectin from Colocasia esculenta (L.) Schott corms and evaluation of its anti-insect potential towards Bactrocera cucurbitae (Coquilett). The lectin was found to be specific towards N-acetyl-D-lactosamine (LacNac), a disaccharide and asialofetuin, a desialylated serum glycoprotein in hemagglutination inhibition assay. Asialofetuin was used as a ligand to purify Colocasia esculenta agglutinin (CEA) by affinity chromatography. The purity of CEA was ascertained by the presence of a single band in reducing SDS-PAGE at pH 8.3. The affinity purified CEA was employed in artificial diet bioassay of second instar larvae (64-72 hr old) of the B. cucurbitae at concentrations ranging between 10-160 microg ml(-1). The lectin significantly (p < 0.01) decreased the percent pupation and emergence with respect to control. Effect on various enzymes was studied by employing LC50 (51.6 microg ml(-1)) CEA in the artificial diet bioassay of second instar larvae. All the enzymes tested namely esterases, phosphatases (acid and alkaline), superoxide dismutases, catalase and glutathione-S-transferase showed a significant (p < 0.01, p < 0.05) increase in their enzyme and specific activities. These results showed that CEA affected normal growth and development and presented stress to the larvae, activating their detoxification and anti-oxidant systems. Thus, the lectin seems to be a useful candidate for the control measures of B. cucurbitae under the integrated pest management (IPM) system.

  19. Novel toxicity of Bacillus thuringiensis strains against the melon fruit fly, Bactrocera cucurbitae (Diptera: Tephritidae).

    Science.gov (United States)

    Shishir, Md Asaduzzaman; Akter, Asma; Bodiuzzaman, Md; Hossain, M Aftab; Alam, Md Musfiqul; Khan, Shakil Ahmed; Khan, Shakila Nargis; Hoq, M Mozammel

    2015-01-01

    Bactrocera cucurbitae (melon fruit fly) is one of the most detrimental vegetable-damaging pests in Bangladesh. The toxicity of Bacillus thuringiensis (Bt) has been reported against a few genera of Bactrocera in addition to numerous other insect species. Bt strains, harbouring cry1A-type genes were, therefore, assayed in vivo against the 3(rd) instar larvae of B. cucurbitae in this study. The biotype-based prevalence of cry1 and cry1A genes was calculated to be 30.8% and 11.16%, respectively, of the test strains (n=224) while their prevalence was greatest in biotype kurstaki. Though three indigenous Bt strains from biotype kurstaki with close genetic relationship exhibited higher toxicity, maximum mortalities were recorded for Btk HD-73 (96%) and the indigenous Bt JSc1 (93%). LC50 and LC99 values were determined to be 6.81 and 8.32 for Bt JSc1, 7.30 and 7.92 for Bt SSc2, and 6.99 and 7.67 for Btk HD-73, respectively. The cause of toxicity and its variation among the strains was found to be correlated with the synergistic toxic effects of cry1, cry2, cry3 and cry9 gene products, i.e. relevant Cry proteins. The novel toxicity of the B. thuringiensis strains against B. cucurbitae revealed in the present study thus will help in developing efficient and eco-friendly control measures such as Bt biopesticides and transgenic Bt cucurbits.

  20. A second gene for acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase in squash, Cucurbita moschata cv. Shirogikuza(*), codes for an oleate-selective isozyme: molecular cloning and protein purification studies.

    Science.gov (United States)

    Nishida, I; Sugiura, M; Enju, A; Nakamura, M

    2000-12-01

    A new isogene for acyl-(acyl-carrier-protein):glycerol-3-phosphate acyltransferase (GPAT; EC 2.3.1.15) in squash has been cloned and the gene product was identified as oleate-selective GPAT. Using PCR primers that could hybridise with exons for a previously cloned squash GPAT, we obtained two PCR products of different size: one coded for a previously cloned squash GPAT corresponding to non-selective isoforms AT2 and AT3, and the other for a new isozyme, probably the oleate-selective isoform AT1. Full-length amino acid sequences of respective isozymes were deduced from the nucleotide sequences of genomic genes and cDNAs, which were cloned by a series of PCR-based methods. Thus, we designated the new gene CmATS1;1 and the other one CmATS1;2. Genome blot analysis revealed that the squash genome contained the two isogenes at non-allelic loci. AT1-active fractions were partially purified, and three polypeptide bands were identified as being AT1 polypeptides, which exhibited relative molecular masses of 39.5-40.5 kDa, pI values of 6.75-7.15, and oleate selectivity over palmitate. Partial amino-terminal sequences obtained from two of these bands verified that the new isogene codes for AT1 polypeptides.

  1. 利用SSR标记技术鉴定南瓜杂交种纯度的研究%The Research of the Cucurbita moschata Hybrids Purity Identification by SSR Makers

    Institute of Scientific and Technical Information of China (English)

    韩小霞; 胡新军; 袁祖华; 李勇奇; 杨建国; 粟建文

    2014-01-01

    为研究SSR标记技术在南瓜杂交种纯度鉴定上的可行性,本研究利用选取的33对南瓜SSR引物,对2个中国南瓜杂交品种(大果蜜本与蜜宝)和2个中国南瓜杂交组合(P12与P13)的杂交种样品进行了纯度鉴定,并与田间形态鉴定结果进行了对比分析.研究结果表明,SSR鉴定与田间种植鉴定的结果差异在0.7%~1.8%之间,相关系数达到0.999,达到极显著相关水平,并进一步建立回归方程.本研究结果证实了SSR标记技术应用于南瓜杂交种纯度鉴定工作的可行性与可靠性,为南瓜杂交种纯度鉴定提供技术与理论支持.

  2. 超甜蜜本南瓜茎尖挥发性成分分析%Analysis on the volatile constituents from stem tip of chaotianmiben Cucurbita moschata Duch.

    Institute of Scientific and Technical Information of China (English)

    卢引; 张橡楠; 李昌勤; 邢晗; 康文艺

    2013-01-01

    目的 分析超甜蜜本南瓜茎尖挥发性成分.方法 采用顶空固相微萃取和气质联用技术(HS-SPME/GC-MS )结合保留指数法,首次并用峰面积归一化法测定相对百分含量.结果 从超甜蜜本南瓜茎尖中鉴定出26个化合物,占总峰面积的98.04%.结论 研究表明植酮含量最高(10.14%),其次为丁羟甲苯(9.24%)、(-)-香叶烯D(8.07%).

  3. Description and management of fruit characters of pumpkin (Cucurbita moschata D.)germplasm resources%南瓜资源果实性状描述和管理研究

    Institute of Scientific and Technical Information of China (English)

    周俊国; 李新峥; 李桂荣; 陈志恒

    2006-01-01

    通过对具有不同果实性状特征的637个南瓜果实进行系统的观察比较,详细描述了18个非数值性状的分类特征,并以此为基础建立了种质资源的计算机管理方案,为南瓜种质资源的筛选和育种提供了一定依据,也为南瓜种质资源的管理提供了有效手段.

  4. 采后南瓜果实中几种营养成分的变化%Changes in Several Nutrient Components in Pumpkin (Cucurbita moschata Duch.) Fruit After Harvest

    Institute of Scientific and Technical Information of China (English)

    李新峥; 范文秀; 刘振威; 沈军

    2005-01-01

    "蜜本"南瓜采后0~150 d期间,其果实中多糖和抗坏血酸含量下降,β-胡萝卜素含量增加;总糖、还原糖和蛋白质含量增加,后期下降;氨基酸含量下降,后期增加.

  5. 南瓜叶浓缩蛋白制品的食用安全性初步评价%Preliminary dietary safety evaluation on protein concentrates from pumpkins (Cucurbita moschata) leaves

    Institute of Scientific and Technical Information of China (English)

    黄威

    2013-01-01

    The dietary safety of pumpkin leaf protein products was evaluated in this experiment by using standard methods and experimental models described in the procedures for toxicological assessment on food safety. The result suggested that the LD50 of pumpkin leaf protein concentrate would be higher than 21.501g/kg·bw, which indicated the non-acute toxicity of the experimental material according to the procedures for toxicological assessment on food safety. And.no abnormality was observed after feeding the experimental animals for 30d. Therefore,it was concluded that the pumpkin leaf protein concentrate experimented should not be acutely toxic to the experimental animals.%采用我国《食品安全性毒理学评价程序》中所规定的标准方法和实验模型,对南瓜叶浓缩蛋白制品的食用安全性进行评价.结果显示,该产品小鼠经口LD50值大于21.501g/kg·bw,属无毒级;小鼠30d喂养未显示任何毒副作用.因此,该产品对实验动物不具有急性毒性作用.

  6. An acorn squash (Cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in Cucurbita

    Science.gov (United States)

    Wyatt, Lindsay E; Strickler, Susan R; Mueller, Lukas A; Mazourek, Michael

    2015-01-01

    Acorn squash (Cucurbita pepo) is an iconic fall vegetable in the United States, known for its unique fruit shape and also prized for its culinary properties. Little is known about the metabolism that underlies the development of fruit quality attributes such as color, sweetness, texture and nutritional qualities in acorn squash, or any other winter squash grown worldwide. To provide insight into winter squash fruit and seed development and add to the genomic resources in the Cucurbita genus, RNA sequencing was used to generate an acorn squash fruit and seed transcriptome from the cultivar Sweet REBA at critical points throughout fruit development. 141 838 600 high-quality paired-end Illumina reads were assembled into 55 949 unigenes. 85% of unigenes with predicted open reading frames had homology with previously identified genes and over 62% could be functionally annotated. Comparison with the watermelon and cucumber genomes provided confirmation that the unigenes are full-length and comprehensive, covering an average of 90% of the coding sequence of their homologs and 72% of the cucumber and watermelon exomes. Key candidate genes associated with carotenoid and carbohydrate metabolism were identified toward a resource for winter squash fruit quality trait dissection. This transcriptome represents a major advance in C. pepo genomics, providing significant new sequence information and revealing the repertoire of genes expressed throughout winter squash fruit and seed development. Future studies on the genetic basis of fruit quality and future breeding efforts will be enhanced by tools and insights developed from this resource. PMID:26504561

  7. Parhadjelia cairinae n. sp. (Nematoda: Habronematoidea: Habronematidae) in the Muscovy duck, Cairina moschata (Linnaeus, 1758) (Aves: Anseriformes: Anatidae), from the area de conservacion Guanacaste, Costa Rica.

    Science.gov (United States)

    Zhang, Luping; Brooks, Daniel R

    2005-04-01

    A new species of Parahadjelia occurs in the muscovy duck, Cairina moschata (Linnaeus, 1758), from the Area de Conservacion Guanacaste, Costa Rica. The new species differs from Parhadjelia neglecta Lent and Freitas, 1939, in the body size, in the spicules shape and ratio of spicules, and in having 2 pairs of sessile papillae near the tail tip of the male. The characters exhibited by P. neglecta and the new species validate its generic status distinct from Hadjelia.

  8. Hosting Capacity of Horticultural Plants for Insect Pests in Brazil Capacidad de Alojamiento de Plantas Hortícolas para Plagas de Insectos en Brasil

    Directory of Open Access Journals (Sweden)

    Germano L.D Leite

    2011-09-01

    Full Text Available Factors such as fertilization, allelochemicals, trichomes, weather, and natural enemies can influence pest populations. Thus, it is necessary to understand the factors that predispose vegetable species to pests and the role of polyculture, crop rotation, and neighboring plants. The objective of this research was to study the hosting capacity for pests of Abelmoschus esculentus (L., Brassica oleracea L. vars. acephala and capitata, Capsicum annuum L., Cucurbita moschata (Duchesne, Cucurbita maxima Duchesne and Cucumis sativus L., Lycopersicon esculentum Mill., Solanum gilo Raddi and Solanum melongena L., and Phaseolus vulgaris L. The higher density of Bemisia tabaci (Genn. adults on C. sativus can be due to the higher amount of pentacosane and octacosane in this plant. The occurrence of Brevicoryne brassicae (L. only in Brassica spp. can be accounted for by the nonacosane of these plants. The low trichome density and greater palmitic acid level can explain the greatest damage by Aphis gossypii Glover in A. esculentum. Empoasca sp. was more frequent in P. vulgaris followed by A. esculentum, which are plants with lower K content. Solanum melongena was attacked more by Hydrangea similis (Walker and Epitrix sp. perhaps because of higher palmitic acid and 11,14,17-eicosatrienoic methyl ester concentrations in their leaves. Frankliniella sp. exhibited more damage in C. sativus probably owing to higher pentacosane and octacosane in its leaves. Sistena sp. was more frequent in C. maxima and had higher octadecane levels and trichome density. The presence of ¥-humulene and hexacosane can explain the damage by Tuta absoluta (Meyrick on L. esculentum.Factores tales como la fertilización, aleloquímicos, tricomas, el clima y los enemigos naturales pueden influir en las poblaciones de plagas. Por lo tanto, es necesario comprender los factores que predisponen a las especies vegetales a las plagas y el papel de policultivos, rotación de cultivos y las plantas

  9. Effects of NaCl stress, Ca2+ and GA3 on seed germination of three vegetables in Cucurbita L.%NaCl胁迫及Ca2+和GA3对南瓜属3种蔬菜种子发芽的影响

    Institute of Scientific and Technical Information of China (English)

    王广印; 韩世栋; 赵一鹏; 周秀梅; 李新峥

    2005-01-01

    研究了NaCl胁迫对南瓜(Cucurbita moschata Duch.)、笋瓜(C. maxima Duch.)和西葫芦(C.pepo L.)种子萌发的影响及不同浓度外源Ca2+和GA3对NaCl胁迫下南瓜种子发芽的效应.结果表明,用30 mmol·L-1NaCl处理,南瓜种子的发芽率高于对照(蒸馏水),而用100和170 mmol·L-1NaCl处理,西葫芦和笋瓜种子的发芽率下降率为负值,表明较低浓度NaCl胁迫可一定程度提高西葫芦、笋瓜和南瓜种子的发芽率;高浓度NaCl胁迫对种子发芽有明显的抑制作用.种子萌发期耐盐能力从大至小依次为西葫芦、南瓜、笋瓜.在170 mmol·L-1NaCl胁迫下,施加5~30 mmol·L-1外源Ca2+或浸种处理,对南瓜种子发芽有促进作用,但高浓度外源Ca2+(≥50 mmol·L-1CaCl2)则具有抑制作用.在170 mmol·L-1NaCl胁迫下,用不同浓度GA3浸种处理,对南瓜种子发芽有抑制作用.

  10. Growth and yield of grafted cucumbers in soil infested with root-knot nematodes

    Directory of Open Access Journals (Sweden)

    Smiljana Goreta Ban

    2014-03-01

    Full Text Available The aim of this study was to determine the effect of rootstocks on the growth and yield of cucumber (Cucumis sativus L. plants in soils infested with root-knot nematodes (Meloidogyne spp. Cucumber 'Adrian' was grown with its own roots or was grafted onto three rootstocks of Lagenariasiceraria (Molina Standi. ('Emphasis', 'S-1', and 'Gourd', two interspecific hybrid rootstocks of Cucurbita maxima Duchesne x C. moschata Duchesne ('Strong Tosa' and 'RS 841 Improved' and zucchini Cucurbita pepo L. ('Romanesco Zucchini'. The experiments were conducted in commercial greenhouse, with cucumber grafted onto three rootstocks in the first season and onto six rootstocks in the second spring-summer season. The number of leaves was considerably affected by the rootstock in both seasons, and was the highest for the plants grafted onto interspecific rootstocks (28.0 in the first and 44.9 in the second season. The plants grafted onto 'Strong Tosa' had higher total number of fruits (19.9 and yield (5.38 kg compared to other rootstocks or non-grafted plants in first season, and the same result was found for two interspecific rootstocks in the second season (6.96 kg and more than 28.9 fruits per plant. The total soluble solids, pH and electrical conductivity of the fruit were not affected by rootstock, while titratable acidity changed with the rootstock type. The grafting of cucumber plants onto different rootstocks was confirmed as an acceptable non-chemical method to compete with the limitations of soils infected with root-knot nematodes, but the effect was highly dependent on the choice of the rootstock.

  11. Estudio de un producto de V gama a partir de calabacín (Cucurbita pepo)

    OpenAIRE

    Sáez Uréndez, Víctor

    2007-01-01

    Actualmente se está estudiando y trabajando con los llamados alimentos de V gama: cuya característica es estar el alimento envasado al vacío y tratado térmicamente. La vida útil suele tener una durada de unas cuatro semanas. Este proyecto consiste en estudiar un producto de la V gama de alimentos, para ello se ha utilizado una hortaliza como es el calabacín (Cucurbita pepo). El objetivo del trabajo es determinar que tratamiento térmico es más idóneo para el calabacín. Para realizar el estudio...

  12. Mathematical modelling of light-induced electric reaction of Cucurbita pepo L. leaves

    Directory of Open Access Journals (Sweden)

    Jan Stolarek

    2014-01-01

    Full Text Available The bioelectRIc reactions of 14-16 day old plants of pumpkin (Cucurbita pepo L. and internodal cells of Nitellopsis obtusa to the action of visible and ultraviolet light (UV-C were studied. The possibility of analyzing the bioelectric reaction of pumpkin plants induced by visible light by means of mathematical modelling using a linear differential equation of the second order was considered. The solution of this equation (positive and negative functions can, in a sufficient way, reflect the participation of H+ and CI- ions in the generation of the photoelectric response in green plant cells.

  13. Three new multiflorane-type triterpenes from pumpkin (Cucurbita maxima) seeds.

    Science.gov (United States)

    Kikuchi, Takashi; Takebayashi, Mika; Shinto, Mayumi; Yamada, Takeshi; Tanaka, Reiko

    2013-05-14

    Three new multiflorane-type triterpenes; 7a-methoxymultiflor-8-ene-3a,29-diol 3-acetate-29-benzoate (1), 7-oxomultiflor-8-ene-3a,29-diol 3-acetate-29-benzoate (2), and multiflora-7,9(11)-diene-3a,29-diol 3-p-hydroxybenzoate-29-benzoate (3), were isolated from seeds of Cucurbita maxima, along with three known compounds. Compound 3 and multiflora-7,9(11)-diene-3a-29-diol 3-benzoate (5) exhibited potent inhibitory effects on melanogenesis, with low cytotoxicities, and 2 exhibited single-digit micromolar cytotoxicity against HL-60 and P388 cells.

  14. [Influence of a variation potential on photosynthesis in pumpkin seedlings (Cucurbita pepo L.)].

    Science.gov (United States)

    Sukhov, V S; Shesterneva, O N; Surova, L M; Rumiantsev, E A; Vodeneev, V A

    2013-01-01

    The influence of a variation potential on photosynthesis in pumpkin seedlings (Cucurbita pepo L.) was investigated in our work. It was shown that the variation potential induced by cotyledon burning propagates into a leaf. It decreases CO2 assimilation and transpiration as well as increases nonphotochemical quenching. Investigation of isolated chloroplasts showed that lowering of the pH in incubation medium from 6.9-7.2 to 6.5 increases nonphotochemical quenching. It was proposed that lowering of the cytoplasmic pH induced by the variation potential takes place in the photosynthetic response development.

  15. Two new ent-kaurane-type diterpene glycosides from zucchini (Cucurbita pepo L.) seeds.

    Science.gov (United States)

    Kikuchi, Takashi; Ando, Hiromi; Maekawa, Ken-Ichiro; Arie, Hiroki; Yamada, Takeshi; Tanaka, Reiko

    2015-12-01

    Two new ent-kaurane diterpene glycosides; 12α-(β-d-glucopyranosyloxy)-7β-hydroxykaurenolide (1) and 7β-(β-d-glucopyranosyloxy)-12α-hydroxykaurenolide (2), a new steroid; (24S)-stigmasta-7,22E,25-trien-3-one (12), and known compounds (3-11, 13-14) were isolated from zucchini (Cucurbita pepo L.) seeds. The absolute structures of 1 and 2 were determined by acid hydrolysis and application of a modified Moscher's method. Furthermore, isolated compounds (1-14), and a derivative, 1a, were evaluated for their inhibitory effects on macrophage activation by an inhibitory assay of nitric oxide (NO) production.

  16. Novel Pseudomonas syringae strains associated with leaf spot diseases on watermelon (Citrullus lanatus) and squash (Cucurbita pepo) in California

    Science.gov (United States)

    In 2006 and 2011, bacteria, fluorescent on KMB, were isolated from leaf spots of greenhouse-grown watermelon (Citrullus lanatus) and field-grown squash (Cucurbita pepo) in coastal California. Biochemical characterization of the isolates indicated that they belonged to Pseudomonas syringae. Multilocu...

  17. Response of the pearly eye melon fly Bactrocera cucurbitae(Coquillett)(Diptera:Tephritidae) mutant to host-associated visual cues

    Science.gov (United States)

    We report on a pearly eye mutant (PEM) line generated from a single male Bactrocera cucurbitae collected in Kapoho, Hawaii. Crossing experiments with colony wild-type flies indicate that the locus controlling this trait is autosomal and the mutant allele is recessive. Experiments with females to ass...

  18. Attraction of wild-like and colony-reared Bactrocera cucurbitae (Diptera: Tephritidae) to Cuelure in the field

    Science.gov (United States)

    The attraction of wild tephritids to semiochemical-based lures are the ideal basis for trap network design in detection programs, but in practice, mass-reared colony insects are usually used to determine trap efficiency. For Bactrocera cucurbitae Coquillett, a lower response by wild males compared w...

  19. Transgenic virus resistance in crop-wild Cucurbita pepo does not prevent vertical transmission of zucchini yellow mosaic virus

    Science.gov (United States)

    H. E. Simmons; Holly Prendeville; J. P. Dunham; M. J. Ferrari; J. D. Earnest; D. Pilson; G. P. Munkvold; E. C. Holmes; A. G. Stephenson

    2015-01-01

    Zucchini yellow mosaic virus (ZYMV) is an economically important pathogen of cucurbits that is transmitted both horizontally and vertically. Although ZYMV is seed-transmitted in Cucurbita pepo, the potential for seed transmission in virus-resistant transgenic cultivars is not known. We crossed and backcrossed a transgenic...

  20. Genetic relationship of the melon fly, Bactrocera cucurbitae (Diptera: Tephritidae) inferred from mitochondrial DNA

    Institute of Scientific and Technical Information of China (English)

    Yi Wu; Bruce A. McPheron; Jia-Jiao Wu; Zhi-Hong Li

    2012-01-01

    The melon fruit fly,Bactrocera cucurbitae (Coquillett) (Diptera:Tephritidae),has been the subject of worldwide quarantine and management efforts due to its widespread agricultural impact and potential for rapid range expansion.From its presumed native distribution in India,this species has spread throughout the hot-humid regions of the world.We provide information that reveals population structure,invasion history and population connectivity from 23 locations covering nine countries based on DNA sequences of the mitochondrial cytochrome oxidase I (COI) gene.Forty-two polymorphic sites were described among 38 haplotypes.The most common haplotype,H1,was observed in 73% of the samples distributed among all populations.Highest genetic diversity was seen within populations,and no isolation-by-distance was detected.The western regions (Nepal,Bangladesh,Thailand,Burma and China-west) showed higher haplotype diversity than eastern regions (Chins-east).China-Yunnan showed highest levels of genetic diversity in China.Haplotype diversity decreased with longitude from west to east.Together,these analyses suggest that B.cucurbitae has expanded from west to east within a limited geographic scale and recently invaded China through Yunnan Province.

  1. Nectar defense and hydrogen peroxide in floral nectar of Cucurbita pepo

    Directory of Open Access Journals (Sweden)

    Daniele Nocentini

    2015-03-01

    Full Text Available This study was carried out to investigate some similarities between the nectaries of Nicotiana sp. and Cucurbita pepo, such as starch accumulation in the nectary parenchyma, changes in nectary color during maturation, and the production of a large quantity of sucrose-dominant nectar. The concentration of hydrogen peroxide in C. pepo floral nectar was determined in order to verify the presence of a defense mechanism similar to that found in Nicotiana sp. which protects nectar from yeast and bacteria proliferation. We also tested the eventual accumulation of antioxidants in the nectary of C. pepo as a protection against oxidative stress caused by hydrogen peroxide. The level of hydrogen peroxide found in the floral nectar of C. pepo was much lower than that found in Nicotiana sp. and the male flowers of Cucurbita had a higher concentration than the female flowers. The low oxidative stress induced by this level of hydrogen peroxide caused the accumulation of a low amount of lutein inside the plastoglobules which were contained in amyloplasts. Plastids of the C. pepo nectary are specialized in the accumulation of starch rather than antioxidants.

  2. Pollination value of male bees: the specialist bee Peponapis pruinosa (Apidae) at summer squash (Cucurbita pepo).

    Science.gov (United States)

    Cane, James H; Sampson, Blair J; Miller, Stephanie A

    2011-06-01

    Male bees can be abundant at flowers, particularly floral hosts of those bee species whose females are taxonomic pollen specialists (oligolecty). Contributions of male bees to host pollination are rarely studied directly despite their prevalence in a number of pollination guilds, including those of some crop plants. In this study, males of the oligolectic bee, Peponapis pruinosa Say, were shown to be effective pollinators of summer squash, Cucurbita pepo L. Seven sequential visits from male P. pruinosa maximized squash fruit set and growth. This number of male visits accumulated during the first hour of their foraging and mate searching at flowers soon after sunrise. Pollination efficacy of male P. pruinosa and their abundances at squash flowers were sufficient to account for most summer squash production at our study sites, and by extrapolation, to two-thirds of all 87 North American farms and market gardens growing squashes that were surveyed for pollinators by collaborators in the Squash Pollinators of the Americas Survey. We posit that the substantial pollination value of male Peponapis bees is a consequence of their species' oligolecty, their mate seeking strategy, and some extreme traits of Cucurbita flowers (massive rewards, flower size, phenology).

  3. IMMUNOCYTOLOGICAL LOCALIZATION OF IAA IN THE PARENCHYMA CELL AND VASCULAR ELEMENTS IN THE GRAFT UNION OF CUCURBITA PEPO/CUCURBITA MOSCHATA AT THE EARLY DEVELOPMENTAL STAGE%嫁接早期IAA在西葫芦/南瓜嫁接面处薄壁细胞和维管分子中的免疫细胞学定位

    Institute of Scientific and Technical Information of China (English)

    王幼群; 韩静; 贾文锁; 杜中

    2000-01-01

    采用免疫胶体金法对IAA在西葫芦/南瓜离体茎段嫁接早期发育时期在嫁接面处的分布进行了超微结构水平的定位.电镜观察表明:在嫁接面处的薄壁细胞中IAA主要定位于细胞核、质体、内质网等细胞器上.在高尔基体、线粒体、细胞壁和液泡中,未发现胶体金颗粒的标记.在分化中的管状分子中,胶体金颗粒位于次生壁上和细胞质中.在筛分子分化过程中,IAA主要定位于筛板、筛孔和细胞质中.在伴胞中有较高的金颗粒密度.对于IAA在嫁接体维管分子分化过程中的作用进行了讨论.

  4. 低温胁迫对砧木南瓜F1代及其亲本幼苗的影响%Effects of Low Temperature Stress on Seedlings of Rootstoch Hybrid F1 (Cucurbita maxima × Cucurbita moschata) and Their Parents

    Institute of Scientific and Technical Information of China (English)

    孙吉庆; 孙令强; 李敏

    2013-01-01

    以印度南瓜(母本,FP)、中国南瓜(父本,MP)及其种间杂交F1(印度南瓜×中国南瓜)代幼苗为试材,采用5℃低温处理幼苗2d,分别测定低温胁迫前后幼苗根系活力、电导率和丙二醛(MDA)含量以及保护酶活性的变化.结果表明:低温胁迫后F1代的根系活力最强,相对电导率最低,MDA含量最低,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性最高,其次是母本印度南瓜,父本中国南瓜最低.这些生理指标的变化与砧木南瓜的耐寒性密切相关,即F1代的耐寒性最强,印度南瓜次之,中国南瓜最弱.

  5. The Respons of Pumpkin Seedlings of Rootstock Hybrid F1 (Cucurbita maxima × Cucurbita moschata) and their Parents to Salt Stress%砧木南瓜F1代及其亲本幼苗对盐胁迫的响应

    Institute of Scientific and Technical Information of China (English)

    赵青华

    2014-01-01

    以青农南瓜砧木1、砧木2及其亲本父本1、父本2、母本1、母本2幼苗为试材,进行100 mmol/LNaCl胁迫,研究胁迫对南瓜幼苗过氧化物酶(POD)、过氧化氢酶(CAT)、脯氨酸及可溶性蛋白质的影响.结果表明,NaCl胁迫使各砧木南瓜幼苗的生长显著受到抑制,处理后幼苗的POD、CAT活性及脯氨酸、可溶性蛋白质含量均比对照高.综合各指标的测定结果认为,母本2幼苗的耐盐性相对较强,砧木处于中间.可见,最耐盐的南瓜品种是青农大的母本2.

  6. Polinização entomófila em abóbora caipira, Cucurbita mixta (Curcubitaceae = Entomophilus pollination in pumpkins, Cucurbita mixta (Curcubitaceae

    Directory of Open Access Journals (Sweden)

    Luiz Henrique Lattaro

    2006-10-01

    Full Text Available O objetivo do presente estudo foi avaliar os insetos visitantes nas flores masculinas e femininas da abóbora caipira (Cucurbita mixta, em Ribeirão Preto, Estado de São Paulo. Para isso, foram realizadas contagens dos insetos presentes nas flores das 7h00 às 18h00, durante 10 minutos em cada horário, com três repetições (três dias distintos, em agosto de 2004. Observou-se que abelha africanizada (Apis mellifera foi o inseto visitante mais freqüente nas flores. Essa abelha visitou as flores até 13h00 e preferiu coletar néctar nasflores masculinas (68,4%, em comparação ao pólen nas flores masculinas (18,6% e ao néctar nas flores femininas (13,0%. A freqüência dessas abelhas nas flores diminuiu no decorrer do dia. A relação de flor masculina para feminina foi 3,2:1.This study aimed to evaluate the insect visitors in the male and female flowers of the pumpkin, Cucurbita mixta, in Ribeirão Preto, São Paulo State, Brazil. The experiment was conducted in August, 2004. The insects in the flowers were counted from 7:00 a.m. to 6:00p.m., for 10 minutes, every hour, with three replications and in three different days. It was observed that the insect which most frequently visited the flowers was the Africanized honey bee, Apis mellifera. That bee visited flowers until 1:00 p.m. and preferred to collect nectar from the male flowers (68.4%, when compared to pollen from the male flowers (18.6% and nectar from the female flowers (13.0%. The frequency of those bees in the flowers decreased in elapsing of the day. The relationship between male and female flowers was 3.2:1.

  7. 黑籽南瓜种间杂交研究%Research of Distant Hybridization Between Fig-leaf Gourd (Cucurbita ficifolia)and Three Mainly Cultivated Species in Cucurbita

    Institute of Scientific and Technical Information of China (English)

    马海龙; 智海英; 岳青

    2011-01-01

    Asynchronous flowering period in different species of Cucurbits was overcome by the means of adjusting sowing time. The experiment of interspecific hybridization was done between 12 materials of three mainly cultivated species (C. Pepo, C. Moschata and C. Maxima) and fig-leaf gourd (C. Ficifolia.). It was no fruit when the fig-leaf gourd act as female parent, and it was fruiting when the other three mainly cultivated species did. There were no seeds in the fruits when C. pepoxC. Gcifolia and C. maximaxC. Gcifolia. 18 Fi plants of C. moschataxC. Gcifolia were obtained. The stamens were degenerate in the plants of the interspecific hybrids Fi. It was fruiting but no seeds when the interspecific hybrids Fi as female parent and backcrossing using C. Moschata or the C. Gcifolia as male parent. The plant morphology of Fi of C. Moschata X C. Gcifolia was intermediate type of parents and had a tendency to the male parent. Disease resistance and insect resistance of the Fi were worse than C. Gcifolia of male parent and approached C. Moschata of female parent.%通过调整播期克服了南瓜属种间花期不遇的问题,以美洲南瓜、中国南瓜、印度南瓜3个种共12个材料与黑籽南瓜进行了种间杂交试验.黑籽南瓜为母本不结实,以其他3个种为母本则均可结实.美洲南瓜×黑籽南瓜及印度南瓜×黑籽南瓜杂交获得的果实内均无种子.获得了18株中国南瓜×黑籽南瓜F1植株.种间杂种F1植株雄蕊退化,以其作为母本以中国南瓜或黑籽南瓜为父本进行回交,可结实但无种子.中国南瓜×黑籽南瓜F1形态为双亲中间型偏父本,其抗病性、抗虫性不及父本黑籽南瓜而倾向于母本中国南瓜.

  8. Monitoring Resistance to Spinosad in the Melon Fly (Bactrocera cucurbitae in Hawaii and Taiwan

    Directory of Open Access Journals (Sweden)

    Ju-Chun Hsu

    2012-01-01

    Full Text Available Spinosad is a natural insecticide with desirable qualities, and it is widely used as an alternative to organophosphates for control of pests such as the melon fly, Bactrocera cucurbitae (Coquillett. To monitor the potential for development of resistance, information about the current levels of tolerance to spinosad in melon fly populations were established in this study. Spinosad tolerance bioassays were conducted using both topical applications and feeding methods on flies from field populations with extensive exposure to spinosad as well as from collections with little or no prior exposure. Increased levels of resistance were observed in flies from the field populations. Also, higher dosages were generally required to achieve specific levels of mortality using topical applications compared to the feeding method, but these levels were all lower than those used for many organophosphate-based food lures. Our information is important for maintaining effective programs for melon fly management using spinosad.

  9. Degradation of disperse dye from textile effluent by free and immobilized Cucurbita pepo peroxidase

    Science.gov (United States)

    Boucherit, N.; Abouseoud, M.; Adour, L.

    2012-06-01

    Disperse dyes constitute the largest group of dyes used in local textile industry. This work evaluates the potential of the Cucurbita peroxidase(C-peroxidase) extracted from courgette in the decolourization of disperse dye in free and immobilized form. The optimal conditions for immobilization of C-peroxidase in Ca-alginate were identified. The immobilization was optimized at 2%(w/v) of sodium alginate and 0.2 M of calcium chloride. After optimization of treatment parameters, the results indicate that at pH 2, dye concentration: 80 mg/L(for FCP) and 180 mg/L(for ICP), H2O2 dose: 0,02M (for FCP) and 0,12M(for ICP), the decolourization by free and immobilized C-peroxidase were 72.02% and 69.71 % respectively. The degradation pathway and the metabolic products formed after the degradation were also predicted using UV-vis spectroscopy analysis.

  10. CHEMICAL WEED CONTROL IN OIL PUMPKIN (CUCURBITA PEPO L. VAR. OLEIFERA PIETSCH

    Directory of Open Access Journals (Sweden)

    Zdenko Besek

    2012-06-01

    Full Text Available A two-year experiment (2002 – 2003 was conducted in oil pumpkin (Cucurbita pepo var. oleifera at two localities (Vranjevo and Poljanice to evaluate the effectiveness of chemical weed control through application of herbicides, and to compare it with mechanical weed control. Main weeds were Amaranthus retroflexus, Setaria viridis and Echinochloa crus-galli. All herbicide treatments, except combination of prometrine + fluazifop-p butyl36, in 2002 appeared to be acceptable to high efficacy in control of annual grass- and broad-leaved weeds. The efficacy of all herbicide treatments in 2003 was lower. The best results of herbicide effectiveness were achieved with combination of linuron + dimethenamid (91.3%, trifluralin (90.1% + napropamide and clomazone + linuron (89%.

  11. Comparison of Enzymatic and Ultrasonic Extraction of Albumin from Defatted Pumpkin (Cucurbita pepo Seed Powder

    Directory of Open Access Journals (Sweden)

    Gia Loi Tu

    2015-01-01

    Full Text Available In this study, ultrasound- and enzyme-assisted extractions of albumin (water-soluble protein group from defatted pumpkin (Cucurbita pepo seed powder were compared. Both advanced extraction techniques strongly increased the albumin yield in comparison with conventional extraction. The extraction rate was two times faster in the ultrasonic extraction than in the enzymatic extraction. However, the maximum albumin yield was 16 % higher when using enzymatic extraction. Functional properties of the pumpkin seed albumin concentrates obtained using the enzymatic, ultrasonic and conventional methods were then evaluated. Use of hydrolase for degradation of cell wall of the plant material did not change the functional properties of the albumin concentrate in comparison with the conventional extraction. The ultrasonic extraction enhanced water-holding, oil-holding and emulsifying capacities of the pumpkin seed albumin concentrate, but slightly reduced the foaming capacity, and emulsion and foam stability.

  12. Sources of resistance in accessions of Cucurbita spp. to virus species from the genus Potyvirus

    Directory of Open Access Journals (Sweden)

    Graziela da Silva Barbosa

    Full Text Available ABSTRACT The identification of source of resistance in cultivated cucurbits species is very important for the development of resistant cultivars to control diseases caused by virus from the genus Potyvirus. The present research had the objective to evaluate the phenotypic reactions and the behavior of pumpkin (Cucurbita spp. accessions to the virus species from the genus Potyvirus: Papaya ringspot virus type Watermelon (PRSV-W, Zucchini yellow mosaic virus (ZYMV and Watermelon mosaic virus (WMV. Twenty-eight accessions of pumpkin from the Cucurbit Germplasm Bank from Embrapa Semiárido, Petrolina, PE, Brazil were evaluated. Twelve young plants from each pumpkin accession were inoculated with each one of the virus species and were maintained at greenhouse for their symptom reaction evaluations. All possible virus infections or absence of infection were confirmed by plate-trapped antigen enzyme linked immunosorbent assay (PTA-ELISA against antisera specific to PRSV-W, ZYMV and WMV at the Plant Virus Laboratory, Universidade Federal do Ceará. Three pumpkin accessions showed extreme resistant to WMV and eight accessions presented mild mosaic when inoculated with PRSV-W. The most severe symptoms were observed on those accessions inoculated with ZYMV and 50% of the accessions showed to be highly susceptible. On the other hand, the pumpkin accessions inoculated with WMV presented the mildest symptoms, indicating that 39% of them were resistant, and 39% were tolerant. The Cucurbita spp. accessions BGC 518, BGC 530, BGC 567, and BGC 683 that showed resistance to one or more than one virus species constitute promising sources of resistance for developing virus resistant pumpkin cultivars or hybrids.

  13. Accumulation of weathered p,p'-DDTs in hybridized Cucurbita pepo cultivars.

    Science.gov (United States)

    Isleyen, Mehmet; Sevim, Pinar; White, Jason C

    2012-08-01

    Cucurbita pepo spp pepo (zucchini) is known as an exceptional weathered dichlorodiphenyldichloroethylene (DDE) accumulator, whereas Cucurbita pepo ssp ovifera (squash) is termed a nonaccumulator. Experiments were conducted with hybridized zucchini and squash to assess the inheritance pattern of DDX (the sum of p,p'-dichlorodiphenyltrichloroethane [p,p'-DDT], p,p'-dichlorodiphenyldichloroethane [p,p'-DDD], and p,p'-dichlorodiphenyldichloroethylene [p,p'-DDE]) accumulation potential in xylem sap and tissues of parental, F1 hybrids, and F1 backcross (BC) generations of plants. Plants were grown in pots containing soil with weathered DDX at 732 to 1,130 ng/g soil or under field conditions in soil with 322 to 2,700 ng/g. The DDX stem bioconcentration factors and xylem sap values showed differences between parental and hybridized plants of squash and zucchini. For squash grown in greenhouse conditions, the DDX flow rate in the xylem sap was 17.3, 121, and 40.8 ng/h in parental, F1 hybrids, and F1 BC plants, respectively. Similarly, the stem DDX content of parental, F1, and F1 BC squash was 11, 253, and 96 ng/g (dry wt), respectively. A similar inheritance pattern for squash was observed when the plants were grown under field conditions. The DDX flow rates in the xylem sap of pot-grown parental, F1, and F1 BC zucchini cultivars were 100, 8.5, and 26 ng/hr, respectively, and the stem DDX content was 191, 102, and 142 ng/g, respectively. Again, similar trends in accumulation potential were observed for hybridized zucchini grown under field conditions. The DDX concentrations in parental plants matched the expected pattern, with hybrids midway between the two species, and the backcross being more like the parent again for both species. This inheritance pattern of contaminant accumulation and translocation ability follows classical Mendelian segregation and suggests single-gene or single-locus control.

  14. Sphingomonas taxi, Isolated from Cucurbita pepo, Proves to Be a DDE-Degrading and Plant Growth-Promoting Strain.

    Science.gov (United States)

    Eevers, Nele; Van Hamme, Jonathan D; Bottos, Eric M; Weyens, Nele; Vangronsveld, Jaco

    2015-05-14

    The draft genome of Sphingomonas taxi, a strain of the Sphingomonadaceae isolated from Cucurbita pepo root tissue, is presented. This Gram-negative bacterium shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (DDE)-degrading potential and plant growth-promoting capacities. An analysis of its 3.9-Mb draft genome will enhance the understanding of DDE-degradation pathways and phytoremediation applications for DDE-contaminated soils. Copyright © 2015 Eevers et al.

  15. Draft Genome Sequence of Enterobacter aerogenes, a DDE-Degrading and Plant Growth-Promoting Strain Isolated from Cucurbita pepo

    OpenAIRE

    Eevers, Nele; Van Hamme, J.D.; Bottos, E.M.; Weyens, Nele; Vangronsveld, Jaco

    2015-01-01

    We report here the draft genome of Enterobacter aerogenes, a Gram-negative bacterium of the Enterobacteriaceae isolated from Cucurbita pepo root tissue. This bacterium shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (DDE)-degrading potential and plant growth-promoting capacity. An analysis of its 4.5-Mb draft genome will enhance the understanding of DDE degradation pathways and phytoremediation applications for DDE-contaminated soils.

  16. Insights into the evolution of mitochondrial genome size from complete sequences of Citrullus lanatus and Cucurbita pepo (Cucurbitaceae).

    Science.gov (United States)

    Alverson, Andrew J; Wei, XiaoXin; Rice, Danny W; Stern, David B; Barry, Kerrie; Palmer, Jeffrey D

    2010-06-01

    The mitochondrial genomes of seed plants are unusually large and vary in size by at least an order of magnitude. Much of this variation occurs within a single family, the Cucurbitaceae, whose genomes range from an estimated 390 to 2,900 kb in size. We sequenced the mitochondrial genomes of Citrullus lanatus (watermelon: 379,236 nt) and Cucurbita pepo (zucchini: 982,833 nt)--the two smallest characterized cucurbit mitochondrial genomes--and determined their RNA editing content. The relatively compact Citrullus mitochondrial genome actually contains more and longer genes and introns, longer segmental duplications, and more discernibly nuclear-derived DNA. The large size of the Cucurbita mitochondrial genome reflects the accumulation of unprecedented amounts of both chloroplast sequences (>113 kb) and short repeated sequences (>370 kb). A low mutation rate has been hypothesized to underlie increases in both genome size and RNA editing frequency in plant mitochondria. However, despite its much larger genome, Cucurbita has a significantly higher synonymous substitution rate (and presumably mutation rate) than Citrullus but comparable levels of RNA editing. The evolution of mutation rate, genome size, and RNA editing are apparently decoupled in Cucurbitaceae, reflecting either simple stochastic variation or governance by different factors.

  17. Can Adverse Effects of Acidity and Aluminum Toxicity be Alleviated by Appropriate Rootstock Selection in Cucumber?

    Directory of Open Access Journals (Sweden)

    Youssef Rouphael

    2016-08-01

    Full Text Available Low-pH and aluminium (Al stresses are the major constraints that limit crop yield in acidic soils. Grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and Al toxicity. Two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition and assimilate partitioning in plant tissues of cucumber plants (Cucumis sativus L.‘Ekron’ either non-grafted or grafted onto ‘P360’ (Cucurbita maxima Duchesne × Cucurbita moschata Duchesne; E/C or figleaf gourd (Cucurbita ficifolia Bouché; E/F. Cucumber plants were cultured in pots and supplied with nutrient solutions having different pH and Al concentrations: pH 6, pH 3.5, pH 3.5 + 1.5 mM Al and pH 3.5 + 3 mM Al (Exp. 1, 14 d and pH 6, pH 3.5 and pH 3.5 +0.75 mM Al (Exp. 2, 67 d. Significant depression in shoot and root biomass was observed in response to acidity and Al concentrations, with Al-stress being more phytotoxic than low pH treatment. Significant decrease in yield, shoot and root biomass, leaf area, SPAD index, N, K, Ca, Mg, Mn, and B concentration in aerial parts (leaves and stems in response to low pH with more detrimental effects at pH 3.5 + Al. Grafted E/C plants grown under low pH and Al had higher yield, shoot and root biomass compared to E/F and non-grafted plants. This better crop performance of E/C plants in response to Al stress was related to i a reduced translocation of Al from roots to the shoot, ii a better shoot and root nutritional status in K, Ca, Mg, Mn, and Zn concentration, iii a higher chlorophyll synthesis, as well as iv the ability to maintain cell membrane stability and integrity (lower electrolyte leakage. Data provide insight into the role of grafting on Al stress tolerance in cucumber.

  18. Can Adverse Effects of Acidity and Aluminum Toxicity Be Alleviated by Appropriate Rootstock Selection in Cucumber?

    Science.gov (United States)

    Rouphael, Youssef; Rea, Elvira; Cardarelli, Mariateresa; Bitterlich, Michael; Schwarz, Dietmar; Colla, Giuseppe

    2016-01-01

    Low-pH and aluminum (Al) stresses are the major constraints that limit crop yield in acidic soils. Grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and Al toxicity. Two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition, and assimilate partitioning in plant tissues of cucumber plants (Cucumis sativus L. "Ekron") either non-grafted or grafted onto "P360" (Cucurbita maxima Duchesne × Cucurbita moschata Duchesne; E/C) or figleaf gourd (Cucurbita ficifolia Bouché; E/F). Cucumber plants were cultured in pots and supplied with nutrient solutions having different pH and Al concentrations: pH 6, pH 3.5, pH 3.5 + 1.5 mM Al, and pH 3.5 + 3 mM Al (Experiment 1, 14 days) and pH 6, pH 3.5, and pH 3.5 + 0.75 mM Al (Experiment 2, 67 days). Significant depression in shoot and root biomass was observed in response to acidity and Al concentrations, with Al-stress being more phytotoxic than low pH treatment. Significant decrease in yield, shoot, and root biomass, leaf area, SPAD index, N, K, Ca, Mg, Mn, and B concentration in aerial parts (leaves and stems) in response to low pH with more detrimental effects at pH 3.5 + Al. Grafted E/C plants grown under low pH and Al had higher yield, shoot, and root biomass compared to E/F and non-grafted plants. This better crop performance of E/C plants in response to Al stress was related to (i) a reduced translocation of Al from roots to the shoot, (ii) a better shoot and root nutritional status in K, Ca, Mg, Mn, and Zn concentration, (iii) a higher chlorophyll synthesis, as well as (iv) the ability to maintain cell membrane stability and integrity (lower electrolyte leakage). Data provide insight into the role of grafting on Al stress tolerance in cucumber.

  19. Effects of pollen selection on progeny vigor in a Cucurbita pepo x C. texana hybrid.

    Science.gov (United States)

    Quesada, M; Winsor, J A; Stephenson, A G

    1996-05-01

    We examined the effects of pollen selection for rapid pollen-tube growth on progeny vigor. First, we crossed a wild gourd (Cucurbita texana) to a cultivated zucchini (Cucurbita pepo cv 'Black Beauty') to produce an F1 and then an F2 generation. Half of the F1 seeds were produced by depositing small loads of C. texana pollen onto the stigmas of C. pepo. These small pollen loads were insufficient to produce a full complement of seeds and, consequently, both the fast- and the slow-growing pollen tubes were permitted to achieve fertilization. An F2 generation was then produced by depositing small loads of F1 pollen onto stigmas of F1 plants. The F2 seeds resulting from two generations of small pollen loads are termed the non-selected line because there was little or no selection for pollen-tube growth rate on these plants. The other half of the F1 and F2 seeds were produced by depositing large pollen loads (>10 000 pollen grains) onto stigmas and then allowing only the first 1% or so of the pollen tubes that entered the ovary to fertilize the ovules. We did this by excising the styles at the ovary at 12-15 h after pollination. The resulting F2 seeds are termed 'the selected line' because they were produced by two generations of selection for only the fastest growing pollen tubes. Small pollen loads from the F2plants, both the selected and the non-selected lines, were then deposited onto stigmas of different C. pepo flowers, and the vigor of the resulting seeds was compared under greenhouse and field conditions. The results showed that the seeds fertilized by pollen from the selected line had greater vegetative vigor as seedlings and greater flower and fruit production as mature plants than the seeds fertilized by pollen from the non-selected line. This study demonstrates that selection for fast pollen-tube growth (selection on the microgametophyte) leads to a correlated increase in sporophyte (progeny) vigor.

  20. Plant growth regulators induced urease activity in Cucurbita pepo L. cotyledons.

    Science.gov (United States)

    El Shora, Hamed M; Ali, Awatif S

    2016-03-01

    This study is aimed to investigate the activity of urease (EC 3.5.1.5, urea amidohydrolase) that catalyzes the hydrolysis of urea in 5-day-old Cucurbita pepo cotyledons subjected to various concentrations of different growth regulators. The treatment of C. pepo cotyledons with different concentrations (100-600 μmol) of different auxins [indole-3-acetic acid (IAA), indole butyric acid (IBA), indole propionic acid (IPA) and naphthalene acetic acid (NAA)]; or with different concentrations (100-300 μmol) of different cytokinins [kinetin, zeatin and benzyladenine (6-BA)] resulted in a significant increase of urease activity, compared to control. The optimal effects were recorded for each of 500 μmol of IAA and 300 μmol of zeatin treatments. A gradual increase in urease activity was detected in cotyledons treated with various concentrations (0.2-1.0 mM) of 28-homobrassinolide (HBL), in relative to control. A substantial increase in urease activity was observed in cotyledons subjected to different concentrations of triazole (10-60 mg L(-1)), containing either triadimefon (TDM) or hexaconazole (HEX), compared to control. The combination of 300 μmol zeatin with any of protein inhibitors, namely 5-fluorouridine (FUrd), cordycepin and α-amanitin, resulted in the alleviation of their inhibitory effect on the urease activity.

  1. Effects of amendments on the uptake and distribution of DDT in Cucurbita pepo ssp pepo plants

    Energy Technology Data Exchange (ETDEWEB)

    Whitfield Aslund, Melissa L.; Lunney, Alissa I. [Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, ON, K7K 7B4 (Canada); Rutter, Allison [School of Environmental Studies, Biosciences Complex, Queen' s University, Kingston, ON, K7L 3N6 (Canada); Zeeb, Barbara A., E-mail: zeeb-b@rmc.c [Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, ON, K7K 7B4 (Canada)

    2010-02-15

    The effects of soil amendments on the phytoextraction of SIGMADDT (DDT + DDD + DDE) from soil ([SIGMADDT] approx 1500 ng/g) by a pumpkin variety of Cucurbita pepo ssp pepo were tested and the patterns of SIGMADDT storage throughout the plant shoot were examined. The soil amendments did not increase the total amount of SIGMADDT extracted into plant shoots, but new information about SIGMADDT distribution in the plants was obtained. As observed previously, the SIGMADDT concentration in plant leaves (mean 290 ng/g) was significantly lower than in plant stems (mean 2600 ng/g). Further analysis revealed that SIGMADDT composition was consistent throughout the plant shoot and that SIGMADDT concentration in leaves and stems decreased exponentially as distance from the root increased, which was previously unknown. This new information about the patterns of SIGMADDT uptake and translocation within pumpkin plants highlights the need for appropriate plant sampling strategies in future POPs phytoextraction research. - Patterns of SIGMADDT storage in a pumpkin plant are elucidated and specific surfactant and mycorrhizal soil amendments did not increase the total amount of SIGMADDT phytoextracted into plant shoots.

  2. Chemical composition and biological activity of ripe pumpkin fruits (Cucurbita pepo L.) cultivated in Egyptian habitats.

    Science.gov (United States)

    Badr, Sherif E A; Shaaban, Mohamed; Elkholy, Yehya M; Helal, Maher H; Hamza, Akila S; Masoud, Mohamed S; El Safty, Mounir M

    2011-09-01

    The chemical composition and biological activity of three parts (rind, flesh and seeds) of pumpkin fruits (Cucurbita pepo L.) cultivated in Egypt were studied. Chemical analysis of fibre, protein, β-carotene, carbohydrates, minerals and fatty acids present in the rind, flesh, seeds and defatted seeds meal was conducted. Chemical, GC-MS and biological assays of organic extracts of the main fruit parts, rind and flesh established their unique constituents. Chromatographic purification of the extracts afforded triglyceride fatty acid mixture (1), tetrahydro-thiophene (2), linoleic acid (3), calotropoleanly ester (4), cholesterol (5) and 13(18)-oleanen-3-ol (6). GC-MS analysis of the extract's unpolar fraction revealed the existence of dodecane and tetradecane. Structures of the isolated compounds (1-6) were confirmed by NMR and EI-MS spectrometry. Antimicrobial, antiviral and antitumour activities of the fruit parts were discussed. The promising combined extract of rind and flesh was biologically studied for microbial and cytotoxic activities in comparison with the whole isolated components.

  3. Supercritical carbon dioxide extraction of carotenoids from pumpkin (Cucurbita spp.): a review.

    Science.gov (United States)

    Durante, Miriana; Lenucci, Marcello Salvatore; Mita, Giovanni

    2014-04-21

    Carotenoids are well known for their nutritional properties and health promoting effects representing attractive ingredients to develop innovative functional foods, nutraceutical and pharmaceutical preparations. Pumpkin (Cucurbita spp.) flesh has an intense yellow/orange color owing to the high level of carotenoids, mainly α-carotene, β-carotene, β-cryptoxanthin, lutein and zeaxanthin. There is considerable interest in extracting carotenoids and other bioactives from pumpkin flesh. Extraction procedures able to preserve nutritional and pharmacological properties of carotenoids are essential. Conventional extraction methods, such as organic solvent extraction (CSE), have been used to extract carotenoids from plant material for a long time. In recent years, supercritical carbon dioxide (SC-CO2) extraction has received a great deal of attention because it is a green technology suitable for the extraction of lipophylic molecules and is able to give extracts of high quality and totally free from potentially toxic chemical solvents. Here, we review the results obtained so far on SC-CO2 extraction efficiency and quali-quantitative composition of carotenoids from pumpkin flesh. In particular, we consider the effects of (1) dehydration pre-treatments; (2) extraction parameters (temperature and pressure); the use of water, ethanol and olive oil singularly or in combination as entrainers or pumpkin seeds as co-matrix.

  4. Antioxidative activities and phenolic compounds of pumpkin (Cucurbita pepo) seeds and amaranth (Amaranthus caudatus) grain extracts.

    Science.gov (United States)

    Peiretti, Pier Giorgio; Meineri, Giorgia; Gai, Francesco; Longato, Erica; Amarowicz, Ryszard

    2017-01-23

    Phenolic compounds were extracted from pumpkin (Cucurbita pepo) seed and amaranth (Amaranthus caudatus) grain into 80% (v/v) methanol. The extracts obtained were characterised by the contents of total phenolic compounds (TPC), trolox equivalent antioxidant capacity (TEAC), ferric-reducing antioxidant power (FRAP) and antiradical activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH(·)) radical. The content of individual phenolic compounds was determined by HPLC-DAD method. Pumpkin seeds showed the higher content of TPC than that from amaranth. The TEAC values of both extracts were similar each other. The lower value of FRAP was observed for pumpkin seed. Phenolic compound present in amaranth grain exhibited strongest antiradical properties against DPPH radical. Several peaks were present on the HPLC chromatograms of two extracts. The UV-DAD spectra confirmed the presence of vanillic acid derivatives in the amaranth grain. The three main phenolic compound present in pumpkin seed were characterised by UV-DAD spectra with maximum at 258, 266 and 278 nm.

  5. Solution properties of a heteropolysaccharide extracted from pumpkin (Cucurbita pepo, lady godiva).

    Science.gov (United States)

    Song, Yi; Zhao, Jing; Ni, Yuanying; Li, Quanhong

    2015-11-05

    A water-soluble galactoglucofucomannan was extracted from pumpkin (Cucurbita pepo, lady godiva variety). GC-MS analysis indicated that the polysaccharide was composed of 1,6-linked-glucosyl, 1,2,6-linked-mannosyl, 1,3,6-linked-mannosyl, 1,2,6-linked-galactosyl, 1,2,6-linked-galactosyl, terminal fucosyl and terminal glucose. The solution properties of the polysaccharide were studied systematically by using size-exclusion chromatography combined with multi-angle laser light scattering, viscometry and dynamic light scattering at 25 °C. The weight average molecular masses (Mw), intrinsic viscosity [η], radius of gyration (Rg) and hydrodynamic radius (Rh) were found to be 12.7 × 10(5)g/mol, 780 ml/g, 68 nm and 116 nm, respectively. The fraction dimension and value of ρ (Rg/Rh) of the polysaccharide revealed that it existed in a sphere-like conformation in distilled water. The dependence of zero shear specific viscosity on the coil overlap parameter was analyzed using different models. Furthermore, degradation of samples upon autoclaving has been observed and quantified by intrinsic viscosity determination and SEC-MALLS.

  6. Pumpkin (Cucurbita maxima) seed proteins: sequential extraction processing and fraction characterization.

    Science.gov (United States)

    Rezig, Leila; Chibani, Farhat; Chouaibi, Moncef; Dalgalarrondo, Michèle; Hessini, Kamel; Guéguen, Jacques; Hamdi, Salem

    2013-08-14

    Seed proteins extracted from Tunisian pumpkin seeds ( Cucurbita maxima ) were investigated for their solubility properties and sequentially extracted according to the Osborne procedure. The solubility of pumpkin proteins from seed flour was greatly influenced by pH changes and ionic strength, with higher values in the alkaline pH regions. It also depends on the seed defatting solvent. Protein solubility was decreased by using chloroform/methanol (CM) for lipid extraction instead of pentane (P). On the basis of differential solubility fractionation and depending on the defatting method, the alkali extract (AE) was the major fraction (42.1 (P), 22.3% (CM)) compared to the salt extract (8.6 (P), 7.5% (CM)). In salt, alkali, and isopropanol extracts, all essential amino acids with the exceptions of threonine and lysine met the minimum requirements for preschool children (FAO/WHO/UNU). The denaturation temperatures were 96.6 and 93.4 °C for salt and alkali extracts, respectively. Pumpkin protein extracts with unique protein profiles and higher denaturation temperatures could impart novel characteristics when used as food ingredients.

  7. Rapid affinity-purification and physicochemical characterization of pumpkin (Cucurbita maxima) phloem exudate lectin.

    Science.gov (United States)

    Narahari, Akkaladevi; Swamy, Musti J

    2010-04-21

    The chito-oligosaccharide-specific lectin from pumpkin (Cucurbita maxima) phloem exudate has been purified to homogeneity by affinity chromatography on chitin. After SDS/PAGE in the presence of 2-mercaptoethanol, the pumpkin phloem lectin yielded a single band corresponding to a molecular mass of 23.7 kDa, whereas ESI-MS (electrospray ionization MS) gave the molecular masses of the subunit as 24645 Da. Analysis of the CD spectrum of the protein indicated that the secondary structure of the lectin consists of 9.7% alpha-helix, 35.8% beta-sheet, 22.5% beta-turn and 32.3% unordered structure. Saccharide binding did not significantly affect the secondary and tertiary structures of the protein. The haemagglutinating activity of pumpkin phloem lectin was mostly unaffected in the temperature range 4-70 degrees C, but a sharp decrease was seen between 75 and 85 degrees C. Differential scanning calorimetric and CD spectroscopic studies suggest that the lectin undergoes a co-operative thermal unfolding process centred at approx. 81.5 degrees C, indicating that it is a relatively stable protein.

  8. Developmental instability: measures of resistance and resilience using pumpkin (Cucurbita pepo L.)

    Science.gov (United States)

    Freeman, D. Carl; Brown, Michelle L.; Dobson, Melissa; Jordan, Yolanda; Kizy, Anne; Micallef, Chris; Hancock, Leandria C.; Graham, John H.; Emlen, John M.

    2003-01-01

    Fluctuating asymmetry measures random deviations from bilateral symmetry, and thus estimates developmental instability, the loss of ability by an organism to regulate its development. There have been few rigorous tests of this proposition. Regulation of bilateral symmetry must involve either feedback between the sides or independent regulation toward a symmetric set point. Either kind of regulation should decrease asymmetry over time, but only right–left feedback produces compensatory growth across sides, seen as antipersistent growth following perturbation. Here, we describe the developmental trajectories of perturbed and unperturbed leaves of pumpkin, Cucurbita pepoL., grown at three densities. Covering one side of a leaf with aluminium foil for 24 h perturbed leaf growth. Reduced growth on the perturbed side caused leaves to become more asymmetrical than unperturbed controls. After the treatment the size-corrected asymmetry decreased over time. In addition, rescaled range analysis showed that asymmetry was antipersistent rather than random, i.e. fluctuation in one direction was likely to be followed by fluctuations in the opposite direction. Development involves right–left feedback. This feedback reduced size-corrected asymmetry over time most strongly in the lowest density treatment suggesting that developmental instability results from a lack of resilience rather than resistance. 

  9. Carbohydrate metabolism before and after dehiscence in the recalcitrant pollen of pumpkin (Cucurbita pepo L.).

    Science.gov (United States)

    Carrizo García, C; Guarnieri, M; Pacini, E

    2015-05-01

    Pumpkin (Cucurbita pepo L.) pollen is starchy, sucrose-poor and recalcitrant, features opposite to those of several model species; therefore, some differences in carbohydrate metabolism could be expected in this species. By studying pumpkin recalcitrant pollen, the objective was to provide new biochemical evidence to improve understanding of how carbohydrate metabolism might be involved in pollen functioning in advanced stages. Four stages were analysed: immature pollen from 1 day before anthesis, mature pollen, mature pollen exposed to the environment for 7 h, and pollen rehydrated in a culture medium. Pollen viability, water and carbohydrate content and activity of enzymes involved in carbohydrate metabolism were quantified in each stage. Pollen viability and water content dropped quickly after dehiscence, as expected. The slight changes in carbohydrate concentration and enzyme activity during pollen maturation contrast with major changes recorded with ageing and rehydration. Pumpkin pollen seems highly active and closely related to its surrounding environment in all the stages analysed; the latter is particularly evident among insoluble sucrolytic enzymes, mainly wall-bound acid invertase, which would be the most relevant for sucrose cleavage. Each stage was characterised by a particular metabolic/enzymatic profile; some particular features, such as the minor changes during maturation, fast sucrolysis upon rehydration or sharp decrease in insoluble sucrolytic activity with ageing seem to be related to the lack of dormancy and recalcitrant nature of pumpkin pollen.

  10. Movement of soil-applied imidacloprid and thiamethoxam into nectar and pollen of squash (Cucurbita pepo).

    Science.gov (United States)

    Stoner, Kimberly A; Eitzer, Brian D

    2012-01-01

    There has been recent interest in the threat to bees posed by the use of systemic insecticides. One concern is that systemic insecticides may translocate from the soil into pollen and nectar of plants, where they would be ingested by pollinators. This paper reports on the movement of two such systemic neonicotinoid insecticides, imidacloprid and thiamethoxam, into the pollen and nectar of flowers of squash (Cucurbita pepo cultivars "Multipik," "Sunray" and "Bush Delicata") when applied to soil by two methods: (1) sprayed into soil before seeding, or (2) applied through drip irrigation in a single treatment after transplant. All insecticide treatments were within labeled rates for these compounds. Pollen and nectar samples were analyzed using a standard extraction method widely used for pesticides (QuEChERS) and liquid chromatography mass spectrometric analysis. The concentrations found in nectar, 10 ± 3 ppb (mean ± s.d) for imidacloprid and 11 ± 6 ppb for thiamethoxam, are higher than concentrations of neonicotinoid insecticides in nectar of canola and sunflower grown from treated seed, and similar to those found in a recent study of neonicotinoids applied to pumpkins at transplant and through drip irrigation. The concentrations in pollen, 14 ± 8 ppb for imidacloprid and 12 ± 9 ppb for thiamethoxam, are higher than those found for seed treatments in most studies, but at the low end of the range found in the pumpkin study. Our concentrations fall into the range being investigated for sublethal effects on honey bees and bumble bees.

  11. Characterization and Pathogenicity of Alternaria burnsii from Seeds of Cucurbita maxima (Cucurbitaceae) in Bangladesh.

    Science.gov (United States)

    Paul, Narayan Chandra; Deng, Jian Xin; Lee, Hyang Burm; Yu, Seung-Hun

    2015-12-01

    In the course of survey of endophytic fungi from Bangladesh pumpkin seeds in 2011~2012, two strains (CNU111042 and CNU111043) with similar colony characteristics were isolated and characterized by their morphology and by molecular phylogenetic analysis of the internal transcribed spacer, glyceraldehydes-3-phosphate dehydrogenase (gpd), and Alternaria allergen a1 (Alt a1) sequences. Phylogenetic analysis of all three sequences and their combined dataset revealed that the fungus formed a subclade within the A. alternata clade, matching A. burnsi and showing differences with its other closely related Alternaria species, such as A. longipes, A. tomato, and A. tomaticola. Long ellipsoid, obclavate or ovoid beakless conidia, shorter and thinner conidial size (16~60 [90] × 6.5~14 [~16] µm) distinguish this fungus from other related species. These isolates showed more transverse septation (2~11) and less longitudinal septation (0~3) than did other related species. Moreover, the isolate did not produce any diffusible pigment on media. Therefore, our results reveal that the newly recorded fungus from a new host, Cucurbita maxima, is Alternaria burnsii Uppal, Patel & Kamat.

  12. Morphological and Nutritive Variation in a Collection of Cucurbita pepo L. Growing in Kosova

    Directory of Open Access Journals (Sweden)

    Sali ALIU

    2011-05-01

    Full Text Available Kosovo is the one of the important diversity centers for Cucurbita pepo because of their adaptation to diverse agro ecological conditions as a result of natural and also farmer�s selection. In Kosovo, in most cases C. pepo is grown both in maize fields and vegetable gardens as well as in other more intensive systems. Our expedition have identified different location in the part of Kosovo where were collected a samples at altitudes from 545 till 748 MASL. Kjeldahl method we have used to determine Protein content (PC. Lipids were determined by extraction using Soxhlet apparatus and diethyl ether was used as extraction solvent. The aim of the present investigation was carried out to study morphological diversity, protein and lipids content. Pumpkin populations show great diversity in morphological characteristics, particularly fruit weight (FW, fruit shape (FSH. All populations for FW were on higher genetic variability + 2556.9 g or 97.71%. The collection showed appreciable phenotypic variation in fruit shape (FSH. The greater part of variance was accounted for protein content (PC and lipids content (LC. The estimation overall value ? in seed for PC and LC were 14.59%, and 32.99% respectively. This evaluation of traits variability can assist breeders to identify populations with desirable characteristics for inclusion in variety breeding programs.

  13. Supercritical Carbon Dioxide Extraction of Carotenoids from Pumpkin (Cucurbita spp.: A Review

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    Miriana Durante

    2014-04-01

    Full Text Available Carotenoids are well known for their nutritional properties and health promoting effects representing attractive ingredients to develop innovative functional foods, nutraceutical and pharmaceutical preparations. Pumpkin (Cucurbita spp. flesh has an intense yellow/orange color owing to the high level of carotenoids, mainly α-carotene, β-carotene, β-cryptoxanthin, lutein and zeaxanthin. There is considerable interest in extracting carotenoids and other bioactives from pumpkin flesh. Extraction procedures able to preserve nutritional and pharmacological properties of carotenoids are essential. Conventional extraction methods, such as organic solvent extraction (CSE, have been used to extract carotenoids from plant material for a long time. In recent years, supercritical carbon dioxide (SC-CO2 extraction has received a great deal of attention because it is a green technology suitable for the extraction of lipophylic molecules and is able to give extracts of high quality and totally free from potentially toxic chemical solvents. Here, we review the results obtained so far on SC-CO2 extraction efficiency and quali-quantitative composition of carotenoids from pumpkin flesh. In particular, we consider the effects of (1 dehydration pre-treatments; (2 extraction parameters (temperature and pressure; the use of water, ethanol and olive oil singularly or in combination as entrainers or pumpkin seeds as co-matrix.

  14. Selection of reference genes for gene expression studies in zucchini (Cucurbita pepo) using qPCR.

    Science.gov (United States)

    Obrero, Angeles; Die, Jose V; Román, Belén; Gómez, Pedro; Nadal, Salvador; González-Verdejo, Clara I

    2011-05-25

    The zucchini (Cucurbita pepo) is an important food crop, the transcriptomics of which are a fundamental tool to accelerate the development of new varieties by breeders. However, the suitability of reference genes for data normalization in zucchini has not yet been studied. The aim of this study was to assess the suitability of 13 genes for their potential use as reference genes in quantitative real-time PCR. Assays were performed on 34 cDNA samples representing plants under different stresses and at different developmental stages. The application of geNorm and NormFinder software revealed that the use of a combination of UFP, EF-1A, RPL36aA, PP2A, and CAC genes for the different experimental sets was the best strategy for reliable normalization. In contrast, 18S rRNA and TUA were less stable and unsuitable for use as internal controls. These results provide the possibility to allow more accurate use of qPCR in this horticultural crop.

  15. Interaction of xylem and phloem during exudation and wound occlusion in Cucurbita maxima.

    Science.gov (United States)

    Zimmermann, Matthias R; Hafke, Jens B; van Bel, Aart J E; Furch, Alexandra C U

    2013-01-01

    Collection of cucurbit exudates from cut petioles has been a powerful tool for gaining knowledge on phloem sap composition without full notion of the complex exudation mechanism. Only few publications explicitly mentioned that exudates were collected from the basal side of the cut, which exudes more copiously than the apical side. This is surprising since only exudation from the apical side is supposedly driven by phloem pressure gradients. Composition of carbohydrates and pH values at both wounding sides are equal, whereas protein concentration is higher at the basal side. Apparently, exudation is far more complex than just the delivery of phloem sap. Xylem involvement is indicated by lower protein concentrations after elimination of root pressure. Moreover, dye was sucked into xylem vessels owing to relaxation of negative pressure after cutting. The lateral water efflux from the vessels increases turgor of surrounding cells including sieve elements. Simultaneously, detached parietal proteins (PP1/PP2) induce occlusion of sieve plates and cover wound surface. If root pressure is strong enough, pure xylem sap can be collected after removal of the occlusion plug at the wound surface. The present findings provide a mechanism of sap exudation in Cucurbita maxima, in which the contribution of xylem water is integrated. © 2012 Blackwell Publishing Ltd.

  16. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers.

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    Ferriol, M; Picó, B; Nuez, F

    2003-07-01

    Cucurbita pepo is a highly polymorphic species. The cultivars can be grouped into eight morphotypes in two subspecies, ssp. pepo and ssp. ovifera. A collection of 69 accessions representative of the morphotypes and some unclassified types was used for analysing the morphological and molecular diversity of this species. This collection includes commercial cultivars and Spanish landraces, which represent the great diversification of types that have arisen in Europe after this species arrived from America. For the molecular variability studies, two PCR-based systems were employed, AFLP and SRAP, which preferentially amplify ORFs. Principal coordinates analysis and cluster analysis using the UPGMA method clearly separate the accessions into the two subspecies through the use of both markers. However, the gene diversity and the genetic identity values among morphotypes and subspecies varied between the two marker systems. The information given by SRAP markers was more concordant to the morphological variability and to the evolutionary history of the morphotypes than that of AFLP markers. In ssp. ovifera, the accessions of the different morphotypes were basically grouped according to the fruit colour. This may indicate different times of development and also the extent of breeding in the accessions used. This study has allowed identification of new types that can be employed for the development of new cultivars. The landraces of the spp. ovifera, used as ornamental in Europe, have proved to be of great interest for preserving the diversity of C. pepo.

  17. Genetic diversity in a collection of ornamental squash (Cucurbita pepo L.

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    Mladenović Emina

    2014-01-01

    Full Text Available The aim of this study is collecting and preserving of unique Cucurbita pepo germplasm in Serbia. This study also describes the intraspecific variation of C. pepo within twenty germplasm accessions. Conservation work aimed to develop a representative core collection of C. pepo germplasm, to guide future studies and breeding of its ornamental use. A wide range of C. pepo germplasm was collected from diferent parts of the world. Nineteen variables were recorded in 20 varieties to determine the overall degree of polymorphism and to detect similarities among them. Qualitative traits of fruit such is shape, color and texture, showed immense variation. Coefficient of variation were highest for fruit length, fruit weight and number of fruits per plant (CV=56.69161.32%, while they were the lowest for leaf length (CV=20.65%. Morphological characterization is need to facilitate the use of C. pepo varieties in breeding work. Based on the PCA results, 20 accessions of squash are separate in five groups. Those groups are unique in qualitative and quantitative traits. Knowledge of genetic divergence among varieties is essential for breeding.

  18. Nano-silicon dioxide mitigates the adverse effects of salt stress on Cucurbita pepo L.

    Science.gov (United States)

    Siddiqui, Manzer H; Al-Whaibi, Mohamed H; Faisal, Mohammad; Al Sahli, Abdulaziz A

    2014-11-01

    Research into nanotechnology, an emerging science, has advanced in almost all fields of technology. The aim of the present study was to evaluate the role of nano-silicon dioxide (nano-SiO2 ) in plant resistance to salt stress through improvement of the antioxidant system of squash (Cucurbita pepo L. cv. white bush marrow). Seeds treated with NaCl showed reduced germination percentage, vigor, length, and fresh and dry weights of the roots and shoots. However, nano-SiO2 improved seed germination and growth characteristics by reducing malondialdehyde and hydrogen peroxide levels as well as electrolyte leakage. In addition, application of nano-SiO2 reduced chlorophyll degradation and enhanced the net photosynthetic rate (Pn ), stomatal conductance (gs ), transpiration rate, and water use efficiency. The increase in plant germination and growth characteristics through application of nano-SiO2 might reflect a reduction in oxidative damage as a result of the expression of antioxidant enzymes, such as catalase, peroxidase, superoxide dismutase, glutathione reductase, and ascorbate peroxidase. These results indicate that nano-SiO2 may improve defense mechanisms of plants against salt stress toxicity by augmenting the Pn , gs , transpiration rate, water use efficiency, total chlorophyll, proline, and carbonic anhydrase activity in the leaves of plants.

  19. Expression Profile of Carotenoid Cleavage Dioxygenase Genes in Summer Squash (Cucurbita pepo L.).

    Science.gov (United States)

    González-Verdejo, Clara I; Obrero, Ángeles; Román, Belén; Gómez, Pedro

    2015-06-01

    Carotenoids are important dietary components that can be found in vegetable crops. The accumulation of these compounds in fruit and vegetables is altered by the activity of carotenoid cleavage dioxygenases (CCDs) enzymes that produce their degradation. The aim of this work was to study the possible implication of CCD genes in preventing carotenoid storage in the horticultural crop summer squash (Cucurbita pepo L.). The relationship between the presence of these compounds and gene expression for CCDs was studied in three varieties showing different peel and flesh colour. Expression analysis for the CCD genes CpNCED1, CpNCED2, CpNCED3, CpNCED9, CpCCD1, CpCCD4a, CpCCD4b and CpCCD8 was carried out on different organs and at several fruit developmental stages. The results showed that the CpCCD4a and CpCCD4b genes were highly expressed in the variety with lowest carotenoid content suggesting a putative role in carotenoid accumulation pattern in summer squash fruit.

  20. Movement of soil-applied imidacloprid and thiamethoxam into nectar and pollen of squash (Cucurbita pepo.

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    Kimberly A Stoner

    Full Text Available There has been recent interest in the threat to bees posed by the use of systemic insecticides. One concern is that systemic insecticides may translocate from the soil into pollen and nectar of plants, where they would be ingested by pollinators. This paper reports on the movement of two such systemic neonicotinoid insecticides, imidacloprid and thiamethoxam, into the pollen and nectar of flowers of squash (Cucurbita pepo cultivars "Multipik," "Sunray" and "Bush Delicata" when applied to soil by two methods: (1 sprayed into soil before seeding, or (2 applied through drip irrigation in a single treatment after transplant. All insecticide treatments were within labeled rates for these compounds. Pollen and nectar samples were analyzed using a standard extraction method widely used for pesticides (QuEChERS and liquid chromatography mass spectrometric analysis. The concentrations found in nectar, 10 ± 3 ppb (mean ± s.d for imidacloprid and 11 ± 6 ppb for thiamethoxam, are higher than concentrations of neonicotinoid insecticides in nectar of canola and sunflower grown from treated seed, and similar to those found in a recent study of neonicotinoids applied to pumpkins at transplant and through drip irrigation. The concentrations in pollen, 14 ± 8 ppb for imidacloprid and 12 ± 9 ppb for thiamethoxam, are higher than those found for seed treatments in most studies, but at the low end of the range found in the pumpkin study. Our concentrations fall into the range being investigated for sublethal effects on honey bees and bumble bees.

  1. Dieldrin-dissolving abilities of the xylem saps of several plant families, particularly Cucurbita pepo L.

    Science.gov (United States)

    Murano, Hirotatsu; Otani, Takashi; Seike, Nobuyasu

    2010-10-01

    The uptake ability of hydrophobic organic chemicals by plants and the nature of xylem sap of the plants were studied. The plants were grown in soil contaminated with dieldrin. High amounts of dieldrin were detected in the shoots of Cucurbita pepo and Cucumis sativus, but little was seen in the shoots of Brassica oleracea var. italica, Solanum lycopersicum, Glycine max, Zea mays, and Helianthus annuus. The xylem saps of C. pepo and C. sativus leached dieldrin adsorbed on C8 granules, but those of the other plants did not. The xylem saps of C. pepo and C. sativus eluted high amounts of dieldrin from the size-exclusion chromatography column near the fractions of RNase A (13.7 kDa) after Aprotinin (6.5 kDa), which has a larger molecular weight than dieldrin (381). The enhancement of dieldrin solubility by xylem sap was reduced by proteinase and heating. It was suspected that the protein-like materials in the xylem sap delivered dieldrin from the roots to the shoots. Environ. Toxicol. Chem. 2010;29:2269-2277. © 2010 SETAC.

  2. Insecticidal activity of basil oil, trans-anethole, estragole, and linalool to adult fruit flies of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae.

    Science.gov (United States)

    Chang, Chiou Ling; Cho, Il Kyu; Li, Qing X

    2009-02-01

    Basil oil and its three major active constituents (trans-anethole, estragole, and linalool) obtained from basil (Oscimum basilicum L.) were tested on three tephritid fruit fly species [Ceratitis capitata (Wiedemann), Bactrocera dorsalis (Hendel), and Bactrocera cucurbitae (Coquillett)] for insecticidal activity. All test chemicals acted fast and showed a steep dose-response relationship. The lethal times for 90% mortality/knockdown (LT90) of the three fly species to 10% of the test chemicals were between 8 and 38 min. The toxic action of basil oil in C. capitata occurred significantly faster than in B. cucurbitae but slightly faster than in B. dorsalis. Estragole acted faster in B. dorsalis than in C. capitata and B. cucurbitae. Linalool action was faster in B. dorsalis and C. capitata than in B. cucurbitae. trans-Anethole action was similar to all three species. Methyl eugenol acted faster in C. capitata and B. cucurbitae than in B. dorsalis. When linalool was mixed with cuelure (attractant to B. cucurbitae male), its potency to the three fly species decreased as the concentration of cuelure increased. This was due to linalool hydrolysis catalyzed by acetic acid from cuelure degradation, which was confirmed by chemical analysis. When methyl eugenol (B. dorsalis male attractant) was mixed with basil oil, trans-anethole, estragole, or linalool, it did not affect the toxicity of basil oil and linalool to B. dorsalis, but it did significantly decrease the toxicity of trans-anethole and estragole. Structural similarity between methyl eugenol and trans-anethole and estragole suggests that methyl eugenol might act at a site similar to that of trans-anethole and estragole and serve as an antagonist if an action site exists. Methyl eugenol also may play a physiological role on the toxicity reduction.

  3. Grafting for Management of Southern Root-knot Nematode, Meloidogyne Incognita, in Watermelon

    Science.gov (United States)

    Four bottlegourd (Lagenaria siceraria) cultivars, one squash (Cucurbita moschata x C. maxima) hybrid, four wild watermelon (Citrullus lanatus var. citroides) germplasm lines, and one commercial wild watermelon (C. lanatus var. citroides) cultivar were evaluated as rootstocks for cultivated watermelo...

  4. Grafting – a tool for managing root-knot nematodes in watermelon?

    Science.gov (United States)

    Wild watermelon (Citrullus lanatus var. citroides) germplasm, bottlegourd (Lagenaria siceraria) cultivars, hybrid squash (Cucurbita moschata x C. maxima), and commercial wild watermelon (C. lanatus) were evaluated as rootstocks for cultivated watermelon (C. lanatus var. lanatus) in a field infested ...

  5. Utility of Grafting for Managing Southern Root-knot Nematode, Meloidogyne Incognita, in Watermelon

    Science.gov (United States)

    Four bottlegourd (Lagenaria siceraria) cultivars, one squash (Cucurbita moschata x C. maxima) hybrid, four wild watermelon (Citrullus lanatus var. citroides) germplasm lines, and one commercial wild watermelon (C. lanatus var. citroides) cultivar were evaluated as rootstocks for cultivated watermelo...

  6. Boron excess affects photosynthesis and antioxidant apparatus of greenhouse Cucurbita pepo and Cucumis sativus.

    Science.gov (United States)

    Landi, Marco; Remorini, Damiano; Pardossi, Alberto; Guidi, Lucia

    2013-11-01

    This study aimed to evaluate the behavior of zucchini (Cucurbita pepo L.) and cucumber (Cucumis sativus L.) under boron (B) excess. Plants were grown under greenhouse conditions in a sandy soil-peat mixture using a nutrient solution containing 0.2 (control), 10 and 20 mg L(-1) B. Visible symptoms were quantified and leaf B accumulation, gas exchanges, chlorophyll (Chl) a fluorescence, malondialdehyde by-products and antioxidants were investigated 20 days after the beginning of the treatments. Boron toxicity induced oxidative load and leaf necrotic burns coupled with the reduction of leaf growth and biomass accumulation in both species. Boron excess resulted in a decrease of Chl a/b ratio, potential (Fv/Fm) and actual (ΦPSII) PSII quantum efficiency, photosynthetic rate (Pn), stomatal conductance (gs), and transpiration (E) as well. A general stimulation of the antioxidant enzymes ascorbate peroxidase, catalase and superoxide dismutase was observed, and a significant increase in the oxidized form of ascorbate and glutathione was evidenced for treated plants of both species. A difference between the two species was observed: C. pepo appeared to be more sensitive to B stress being damaged at all B concentration. C. sativus grown at 10 mg L(-1) B in nutrient solution showed some down-regulated mechanisms, i.e. increase in Chl b content and a good photochemical PSII efficiency as well as a higher amount of constitutive antioxidant molecules, that, however, are not sufficient to contrast the negative effects of B.

  7. Kinetics of the extraction of pumpkin seed oil (Cucurbita pepo L by supercritical CO2

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    Sovilj Milan N.

    2005-01-01

    Full Text Available This paper deals with the supercritical carbon dioxide extraction of pumpkin seed oil (Cucurbita pepo L. Supercritical fluid extraction (SFE has been used to obtain total lipid extracts from other oilseeds, and it has also proved successful in the isolation and enrichment of sterols from oilseeds. The SFE of pumpkin seed oil on a laboratory scale was investigated in thus paper, with special interest in the influence of the extraction pressure on the overall yield of pumpkin seed oil. Extractions were carried out at the pressures of 15, 25 and 30 MPa and at 313 K, and at a pressure of 30 MPa and the temperatures 313, 323, and 333 K. The yield of the extractions conducted at 15 MPa was rather low, 0.1814 g oil per 1g of seed feed (18.4% for an extraction time of 14 h. However, extractions at higher pressures yielded greater quantities of the oil; at 22.5 MPa for 9 h, 36.3% of the oil and at 30 MPa for 6 h, 41.0% of the oil. For comparison, hexane extraction of the seed material yielded less than 40% of the oil. Temperature did not influence the extraction yield. At a pressure of 30 MPa, the color of the fractions yielded during successive extraction time intervals varied greatly, from pail yellow (the first 2 h, through orange-yellow (from 2-4 h to red (after 4 h. The experimental results of the oil yields were compared with the data obtained by the mathematical model of Hong et al., presented in the literature.

  8. EFFECT OF SPACING SIZE AND SHAPE ON OIL PUMPKIN YIELD (Cucurbita pepo L. var. oleifera

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    Zvjezdana Augustinović

    2006-12-01

    Full Text Available Effect of spacing size and shape on oil pumpkin yield (Cucurbita pepo L. var. oleifera was investigated in two – year field experiments during 2004 and 2005 on The College of Agriculture at Križevci experimental fields. The investigated planting distances were: 100x100, 140x70, 140 x 50, 80 x 80 and 140x30 cm. The experiment was set out by to the Latin square method with five replications, and data was analyzed using analysis of variance. Austrian cultivar Gleissdorf was tested. Number of fruits per plant, average fruit weight, number of fruits per hectare, yield of fruits per hectare, average seed weight per fruit and seed yield per hectare were established. Highly significant large number of fruits per plant in both years was established at greater spacing size (100x100 cm – 1.43 fruit/plant in 2004; 140x70 cm – 1.1 fruit/plant in 2005. Average fruit weight regularly was higher at greater spacing size (140x70 cm – 4239 g in 2004; and 100x100 cm – 4183 g in 2005 but not significant. Even though the differences were not significant between treatmants in both years the greatest number of fruits per hectare (16 167- 2004; 13 917 – 2005 and fruit yield per hectare (60.1 t/ha – 2004; 54.2 t/ha – 2005 was obtained at spacing 140x30 cm. The highest seed yield per hectare in both years (1397.2 kg/ha – 2004; 1167.6 kg/ha – 2005 was obtained at the same spacing (140x30 cm but high significant only in 2004 year.

  9. Uptake and translocation of p,p'-dichlorodiphenyldichloroethylene supplied in hydroponics solution to Cucurbita.

    Science.gov (United States)

    Gent, Martin P N; White, Jason C; Parrish, Zakia D; Isleyen, Mehmet; Eitzer, Brian D; Mattina, MaryJane Incorvia

    2007-12-01

    Field studies show shoots of zucchini (Cucurbita pepo L.) accumulate various hydrophobic contaminants from soil, although many other plants do not, including cucumber (Cucumis sativus L.). To investigate the mechanism for this uptake, we presented p,p'-dichlorodiphenyldichloroethylene (DDE) to these two species in hydroponics solution. A mixture of DDE bound to Tenax beads stirred with a solution of water passing through a reservoir provided a flowing solution containing DDE at approximately 2 microg/L for many weeks duration. Approximately 90% of the DDE supplied in solution was adsorbed on the roots of both cucumber and zucchini. Less than 10% of the sorbed DDE was released subsequently when clean solution flowed past these contaminated roots for 9 d. The shoots of both species accumulated DDE, but the fraction that moved from the roots to the shoot in zucchini, ranging from 6 to 27% in various trials, was 10-fold greater than that in cucumber, 0.7 to 2%. The gradient in DDE concentration in zucchini tissues was in the order root more more than stem > petiole > leaf blade, indicating the movement was through the xylem in the transpiration stream. Some DDE in leaf blades might have been absorbed from the air, because the concentration in this tissue varied less with time, position in trough, or species, than did DDE in stems and petioles. The remarkable ability of zucchini to translocate DDE could not be attributed to differences in tissue composition, growth rate, distribution of weight among plant parts, or in the leaf area and rate of transpiration of water from leaves. Some other factor enables efficient translocation of hydrophobic organic contaminants in the xylem of zucchini.

  10. Inheritance profile of weathered chlordane and p,p'-DDTs accumulation by Cucurbita pepo hybrids.

    Science.gov (United States)

    Isleyen, Mehmet; Sevim, Pinar; Hawthorne, Joseph; Berger, William; White, Jason C

    2013-01-01

    Cucurbita pepo ssp pepo (zucchini) accumulates significant levels of persistent organic pollutants in its roots, followed by unexpectedly high contaminant translocation to the stems. Most other plant species, including the closely related C. pepo ssp ovifera (squash), do not have this ability. To investigate the mechanism of contaminant accumulation, two cultivars each of parental zucchini and squash, as well as previously created first filial (F1) hybrids and F1 backcrosses (BC) of those parental cultivars, were grown under field conditions in a soil contaminated with weathered chlordane (2.29 microg/g) and DDX residues (0.30 microg/g; sum of DDT, DDE, DDD). The parental zucchini had stem-to-soil bioconcentration factors (BCF, contaminant ratio of stem to soil) for chlordane and DDX of 6.23 and 3.10; these values were 2.2 and 3.7 times greater than the squash, respectively. Chlordane and DDX translocation factors, the ratio of contaminant content in the stems to that in the root, were 2.1 and 3.2 times greater for zucchini than for squash. The parental zucchini and squash also differed significantly in chlordane component ratios (relative amounts of trans-nonachlor [TN], cis-chlordane [CC], trans-chlordane [TC]) and enantiomer fractions for the chiral CC and TC. Hybridization of the parental squash and zucchini resulted in significant differences in contaminant uptake. For both the three separate component ratios (CR) and two sets of enantiomer fraction (EF) values, subspecies specific differences in the parental generation became statistically equivalent in the F1 hybrid zucchini and squash. When backcrossed (BC) with the original parental plants, the zucchini and squash F1 BC cultivars reverted to the statistically distinct CR and EF patterns. This pattern of trait segregation upon hybridization suggests either single gene or single locus control for persistent organic pollutant (POP) uptake ability by C. pepo ssp pepo.

  11. Climatic Suitability of Growing Summer Squash (Cucurbita pepo L. as a Medicinal Plant in Iran

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    Mohammad BANNAYAN

    2011-05-01

    Full Text Available Diversification of production by including a broader range of plant species, can significantly contribute to improve health and nutrition, livelihoods, household food security and ecological sustainability. Exploring the climate impact on any given crop is one of the first priorities to find new suitable areas for production and management of new crops. Summer squash (Cucurbita pepo L. is an economically valuable plant with various medicinal potentials. In order to investigate summer squash cultivation feasibility under Iran�s climate, three main agricultural regions (Azerbaijan, Khorasan and central part of Iran (Fars and Isfahan were selected. These regions suitability for summer squash cultivation were evaluated by considering three vital climate variables encompass temperature, precipitation, and sunshine hours. These regions show distinct and representative climatic conditions of Iran. Annual and growing season average of maximum, minimum, mean temperature, precipitation, and sunshine hours were calculated (May-September for all locations with 44 years historical weather data (1961-2005 for 8 locations (Oroomieh, Tabriz, Khoy, Mashhad, Sabzevar, Birjand, Shiraz and Isfahan, 39 years (1966-2005 for 2 locations (Kashan and Fassa, 28 years (1977-2005 for 4 locations (Ardebil, Abadeh, Bojnurd and Shargh Isfahan and 20 years (1985-2005 for 9 locations (Mahabad, Sarab, Maragheh, Parsabad, Khalkhal, Ferdous, Ghaen, Kashmar and Sarakhs. Climatic demands of summer squash were determined by four years field studies at four different locations in Iran. Our results showed Azerbaijan region has a suitable condition for this crop cultivation especially from precipitation and temperature perspectives. Central part of Iran and Khorasan were also found as partly suitable locations however as they are near to deserts with hotter and drier climate, there might be some other crops considered as priorities in these areas.

  12. Genetic relationships in Cucurbita pepo (pumpkin, squash, gourd) as viewed with high frequency oligonucleotide–targeting active gene (HFO–TAG) markers

    Science.gov (United States)

    Cucurbita pepo is a highly diverse, economically important member of the Cucurbitaceae. C. pepo encompasses hundreds of cultivars of pumpkins, squash, and gourds. Although C. pepo has been scrutinized with various types of DNA markers, the relationships among the cultivar-groups of C. pepo subsp. p...

  13. Insecticidal Activity of Basil Oil, trans-Anethole, Estragole, and Linalool to Adults of Ceratitis capitata, Bactrocera dorsalis, and B. cucurbitae

    Science.gov (United States)

    Pest tephritid fruit flies, Ceratitis capitata (Wiedemann), Bactrocera dorsalis (Hendel), and B. cucurbitae (Cocquillett) are among the species of economic significance. Their management has primarily relied on the use of food baits, male attractants and their combinations with insecticides. Basil o...

  14. Monitoring and Varietal Screening Cucurbit Fruit Fly, Bactrocera cucurbitae Coquillett (Diptera: Tephritidae on Cucumber in Bhaktapur and Kathmandu, Nepal

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    Ranju Maharjan

    2015-12-01

    Full Text Available Monitoring of cucurbit fruit fly by using four different types of traps was conducted in Sipadole VDC of Bhaktapur district during 2012 to observe the population dynamics. Three different types of fruit flies were recorded, in which the number of B. cucurbitae dominated to other species. Only B. cucurbitae damaged the cucumber, which was trapped 92.68%, 87.05%, 90.61%, and 69.38% in cue-lure, banana pulp bait, sticky traps and fly catcher, respectively. The highest number of fruit flies (167.5 male fruit flies/3traps was recorded in cue-lure trap during the first week of September, which coincided with 85.45% RH and 21.67°C and 25.04°C minimum and maximum temperature, respectively. Positive relation of temperature, relative humidity and fruit fly catches was observed. Thus, cue-lure was the most effective traps for monitoring of fruit fly population. In varietal screening, among the six different varieties of cucumber, i.e. Kathmandu local, Kusle, Kamini, Malini, Kasinda and Mahyco Green Long, they were highly significant difference in yield. Kamini gave the highest marketable fruit 26.66 mt/ha yield and the lowest by Kusle (5.05 mt/ha. All the varieties were affected by cucurbit fruit fly. The highest number of unmarketable fruit set was observed in Kamini (22.29 fruits/plant.

  15. Designing a New Nano-Plant Composite of Cucurbita pepo for Wound Repair of Skin in Male Albino Mice: A New Nano Approach for Skin Repair

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    Nooshin Naghsh

    2013-06-01

    Full Text Available Background & Objective : The Cucurbita pepo is one of plants that are functional in traditional therapy. This plant has antioxidant and skin damage repair properties. This study investigated the effect of Cucurbita pepo nano silver as a new nano-plant composition in wound repair skin in male mice.   Materials & Methods: In this investigation, male albino mice were places in 8 groups, each containing 8 animals. Group I – VIII were treated with nano silver (500, 250, and 125 ppm concentrations and different concentrations of extracts [70%, 50%, and 25%] and the control group received a mixture of 25% Cucurbita pepo extract (125 ppm nano silver. The eighth group, as control, was treated with sterile deionizer water after the induction of wound skin. The average diameter of the wounds was measured 28 days after treatment in the control and treatment groups. These data were analyzed using the t-test and ANOVA statistical method.   Results: The results of this study showed that ethanol extraction (80% has its highest repair effect 28 days post treatment. The average diameter of the wounds in the control group was 1.16 ±. 0.46 cm, which was decreased to 0 cm and 0.12 ±. 0.23 cm in the ethanol extract (70% of the Cucurbita pepo and component groups, respectively (p value ≤ 0.01.   Conclusion: In this project, nano silver-Cucurbita pepo ethanol extraction for wound repair in albino male mice was more effective than single materials. These findings show that the repair synergic effects are between alcoholic extract and nano silver in this nano composite.

  16. Unusual ploidy in wild strawberry species

    Science.gov (United States)

    The strawberry genus Fragaria L. (Rosaceae) has a base chromosome number of x = 7 with economically important diploid (F. vesca L.), hexaploid (F. moschata Weston), octoploid (F. ×ananassa Duchesne ex Rozier), or artificially developed decaploid (F. ×vescana Rud. Bauer & A. Bauer) cultivars. Since e...

  17. Breeding biology of muscovy duck (Cairina moschata) under natural incubation: the use of the weibull function and a beta-binomial model to predict nest hatchability

    Science.gov (United States)

    Harun; Draisma; Frankena; Veeneklaas; Van Kampen M

    1999-05-07

    In this paper we tested the Weibull function and beta-binomial distribution to analyse and predict nest hatchability, using empirical data on hatchability in Muscovy duck (Cairina moschata) eggs under natural incubation (932 successfully incubated nests and 11 822 eggs). The estimated parameters of the Weibull function and beta-binomial model were compared with the logistic regression analysis. The maximum likelihood estimation of the parameters was used to quantify simultaneously the influence of the nesting behaviour and the duration of the reproduction cycle on hatchability. The estimated parameters showed that the hatchability was not affected in natural dump nests, but in artificial dump nests and in nests with non-term eggs the hatchability was reduced by 10 and 25%, respectively. Similar results were obtained using logistic regression. Both models provided a satisfactory description of the observed data set, but the beta-binomial model proved to have more parameters with practical and biological meaningful interpretations, because this model is able to quantify and incorporate the unexplained variation in a single parameter theta (which is a variance measure). Copyright 1999 Academic Press.

  18. Comparative Study of Strawberry(Fragaria ananassa Duchesne) Leaves Photosynthetic Capacity at Different Ages%不同叶龄草莓叶片光合能力比较研究

    Institute of Scientific and Technical Information of China (English)

    程建军; 高照全; 吴晓云

    2012-01-01

    [Objective] The essay aims to find out the photosynthetic characteristics of Zhangji strawberry leaves at different ages,and provide a theoretical basis for its high yield and good quality cultivation in protective cultivation. [ Method ] The photo synthetic parameters of Zhangji Strawberry in facility environment were determined,and the amount of chlorophyll in the leaves was compared. [ Result] The results showed the photosynthetic rate of strawberry leaves appeared to be a sine wave in one day. At noon,the "nap" of new leaves was more obvious than that of the other leaves. The response trend of photosynthesis of leaves with different folia ages was more or less the same,in other words,the brighter the sunshine was,the bigger the photosynthetic rate was. According to the result,the photosynthetic rate of function leaves in brighter sunshine was obviously bigger than that of new or older leaves. The average photosynthetic rate of function leaves in the daytime was 7.93 μmol/( m2 · s) ,and the new leaves was 5.24 μmol/( m2 · s),while the older leaves was 3.54 μmol/( m2 ·s). The amount of chlorophyll of function leaves was more and consistene with its strong photosynthetic capacity. [Conclusion] The photosynthetic capacity of function leaves is bigger than that of new leaves and older leaves.%[目的]探索不同叶龄“章姬”草莓(Fragaria ananassa Duchesne)叶片的光合特性,为该品种在保护地条件下的高产优质栽培提供理论依据.[方法]对“章姬”草莓在设施条件下的光合参数进行测定,并对其叶绿素含量进行了比较研究.[结果]草莓的光合速率在一天当中大致呈正弦曲线.在晴天中午时新叶的“午休”比中部和内膛叶片明显.不同叶龄叶片对光照的响应趋势基本一致,光照越强,光合速率越大,功能叶片在高光强下的光合速率明显大于新生叶和老叶.白天功能叶片的平均光合速率为7.93 μmol/(m2·s),新叶为5.24 μmol/(m2

  19. First report of zucchini tigre mosaic virus infecting several cucurbit plants in China

    Science.gov (United States)

    Pumpkin (Cucurbita moschata Duch.), Cucumber (Cucumis sativus Linn.) and Zucchini (Cucurbita pepo Linn.) are important crops in tropical and subtropical regions in the world, and they are popular vegetable crops in China. There are currently 59 viruses known infecting cucurbit plants which including...

  20. Comparison of Enzymatic and Ultrasonic Extraction of Albumin from Defatted Pumpkin (Cucurbita pepo)
Seed Powder.

    Science.gov (United States)

    Tu, Gia Loi; Bui, Thi Hoang Nga; Tran, Thi Thu Tra; Ton, Nu Minh Nguyet; Man Le, Van Viet

    2015-12-01

    In this study, ultrasound- and enzyme-assisted extractions of albumin (water-soluble protein group) from defatted pumpkin (Cucurbita pepo) seed powder were compared. Both advanced extraction techniques strongly increased the albumin yield in comparison with conventional extraction. The extraction rate was two times faster in the ultrasonic extraction than in the enzymatic extraction. However, the maximum albumin yield was 16% higher when using enzymatic extraction. Functional properties of the pumpkin seed albumin concentrates obtained using the enzymatic, ultrasonic and conventional methods were then evaluated. Use of hydrolase for degradation of cell wall of the plant material did not change the functional properties of the albumin concentrate in comparison with the conventional extraction. The ultrasonic extraction enhanced water-holding, oil-holding and emulsifying capacities of the pumpkin seed albumin concentrate, but slightly reduced the foaming capacity, and emulsion and foam stability.

  1. Effect of acetylation on antioxidant and cytoprotective activity of polysaccharides isolated from pumpkin (Cucurbita pepo, lady godiva).

    Science.gov (United States)

    Song, Yi; Yang, Yang; Zhang, Yuyu; Duan, Liusheng; Zhou, Chunli; Ni, Yuanying; Liao, Xiaojun; Li, Quanhong; Hu, Xiaosong

    2013-10-15

    Acetylation of pumpkin (Cucurbita pepo, lady godiva variety) polysaccharide using acetic anhydride with pyridines as catalyst under different conditions was conducted to obtain different degrees of acetylation on a laboratory scale. Furthermore, antioxidant activities and cytoprotective effects of pumpkin polysaccharide and its acetylated derivatives were investigated employing various established in vitro systems. Results showed that addition of pyridine as catalyst could increase the degree of substitution, whereas volume of acetic anhydride had little effect. The acetylated polysaccharides in DPPH scavenging radical activity assay, superoxide anion radical activity assay and reducing power assay exhibited higher antioxidant activity than that of unmodified polysaccharide. H2O2-induced oxidative damages on rat thymic lymphocyte were also prevented by pumpkin polysaccharide and its acetylated derivatives and the derivatives presented higher protective effects. On the whole, acetylated polysaccharide showed relevant antioxidant activity both in vitro and in a cell system.

  2. The effect of pumpkin (Cucurbita pepo L) seeds and L-arginine supplementation on serum lipid concentrations in atherogenic rats.

    Science.gov (United States)

    Abuelgassim, Abuelgassim O; Al-showayman, Showayman I A

    2012-01-01

    The present study aimed to examine the effect of pumpkin (Cucurbita pepo L.) seeds supplementation on atherogenic diet-induced atherosclerosis. Rat were divided into two main groups , normal control and atherogenic control rats , each group composed of three subgroups one of them supplemented with 2% arginine in drinking water and the other supplemented with pumpkin seeds in diet at a concentration equivalent to 2% arginine. Supplementation continued for 37 days. Atherogenic rats supplemented with pumpkin seeds showed a significant decrease (ppumpkin seeds significantly decreased serum concentrations of TC and LDL-C. Our findings suggest that pumpkin seeds supplementation has a protective effect against atherogenic rats and this protective effect was not attributed to the high arginine concentrations in pumpkin seeds.

  3. Two and three dimensional characterization of Zucchini Yellow Mosaic Virus induced structural alterations in Cucurbita pepo L. plants.

    Science.gov (United States)

    Zellnig, Günther; Pöckl, Michael Herbert; Möstl, Stefan; Zechmann, Bernd

    2014-05-01

    Infection of plants by Zucchini Yellow Mosaic Virus (ZYMV) induces severe ultrastructural changes. The aim of this study was to investigate ultrastructural changes during ZYMV-infection in Cucurbita pepo L. plants on the two and three dimensional (2D and 3D) level and to correlate these changes with the spread of ZYMV throughout the plant by transmission electron microscopy (TEM) and image analysis. This study revealed that after inoculation of the cotyledons ZYMV moved into roots [3 days post inoculation (dpi)], then moved upwards into the stem and apical meristem (5 dpi), then into the first true leaf (7 dpi) and could finally be found in all plant parts (9 dpi). ZYMV-infected cells contained viral inclusion bodies in the form of cylindrical inclusions (CIs). These CIs occurred in four different forms throughout the cytosol of roots and leaves: scrolls and pinwheels when cut transversely and long tubular structures and bundles of filaments when cut longitudinally. 3D reconstruction of ZYMV-infected cells containing scrolls revealed that they form long tubes throughout the cytosol. The majority has a preferred orientation and an average length and width of 3 μm and 120 nm, respectively. Image analysis revealed an increased size of cells and vacuoles (107% and 447%, respectively) in younger ZYMV-infected leaves leading to a similar ratio of cytoplasm to vacuole (about 1:1) in older and younger ZYMV-infected leaves which indicates advanced cell growth in younger tissues. The collected data advances the current knowledge about ZYMV-induced ultrastructural changes in Cucurbita pepo. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

  4. Diversity of bacterial communities in the midgut of Bactrocera cucurbitae (Diptera: Tephritidae) populations and their potential use as attractants.

    Science.gov (United States)

    Hadapad, Ashok B; Prabhakar, Chandra S; Chandekar, Snehal C; Tripathi, Jyoti; Hire, Ramesh S

    2016-06-01

    The microbiota plays an important role in insect development and fitness. Understanding the gut microbiota composition is essential for the development of pest management strategies. Midgut bacteria were isolated from nine wild B. cucurbitae populations collected from different agroecological zones of India. These isolates were further studied for attractant potential of fruit fly adults, and the chemical constituents in the supernatants of gut bacteria were analysed. Twenty-six bacterial isolates belonging to the families Enterobacteriaceae, Bacillaceae, Micrococcaceae and Staphylococcaceae were isolated and identified on the basis of 16S rRNA gene sequence analysis. The dominant species in the midgut of melon fly were from the genera Enterobacter (34.6%), Klebsiella (19.2%), Citrobacter (7.7%), Bacillus (15.4%) and Providencia (7.7%), and 3.8% each of Micrococcus, Staphylococcus, Leclercia and Exiguobacterium. Bactrocera cucurbitae and B. dorsalis adults were significantly attracted to bacterial whole cell cultures and their supernatants in the fruit fly attraction bioassays. Bacillus cereus, Enterobacter, Klebsiella, Citrobacter and Providencia species attracted both male and females of Bactrocera species. The supernatants of Klebsiella, Citrobacter and Providencia species attracted a significantly greater number of females than males. The most abundant chemical constituents in supernatants of K. oxytoca and C. freundii were 3-methyl-1-butanol, 2-phenylethanol, butyl isocyanatoacetate, 2-methyl-1-propanol and 3-hydroxy-2-butanone, as identified by gas chromatography-mass spectrometry. The bacterial endosymbionts associated with melon fly exhibited attractant potential which could facilitate eco-friendly insect control strategies. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  5. Evidence for the control of phytolith formation in Cucurbita fruits by the hard rind (Hr) genetic locus: Archaeological and ecological implications.

    Science.gov (United States)

    Piperno, Dolores R; Holst, Irene; Wessel-Beaver, Linda; Andres, Thomas C

    2002-08-06

    Many angiosperms, both monocotyledons and dicotyledons, heavily impregnate their vegetative and reproductive organs with solid particles of silicon dioxide (SiO(2)) known as opaline phytoliths. The underlying mechanisms accounting for the formation of phytoliths in plants are poorly understood, however. Using wild and domesticated species in the genus Cucurbita along with their F(1) and F(2) progeny, we have demonstrated that the production of large diagnostic phytoliths in fruit rinds exhibits a one-to-one correspondence to the lignification of these structures. We propose that phytolith formation in Cucurbita fruits is primarily determined by a dominant genetic locus, called hard rind (Hr), previously shown to code for lignin deposition. If true, this evidence represents a demonstration of genetic control over phytolith production in a dicotyledon and provides considerable support to hypotheses that silica phytoliths constitute another important system of mechanical defense in plants. Our research also identifies Hr as another single locus controlling more than one important phenotypic difference between wild and domesticated plants, and establishes rind tissue cell structure and hardness under the effects of Hr as an important determinant of phytolith morphology. When recovered from pre-Columbian archaeological sites, Cucurbita phytoliths represent genetically controlled fossil markers of exploitation and domestication in this important economic genus.

  6. Captures of Wild Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae (Diptera: Tephritidae) in Traps with Improved Multilure TMR Dispensers Weathered in California.

    Science.gov (United States)

    Vargas, Roger I; Souder, Steven K; Morse, Joseph G; Grafton-Cardwell, Elizabeth E; Haviland, David R; Kabashima, John N; Faber, Ben A; Mackey, Bruce; Cook, Peter

    2016-04-01

    During 2012–2013, solid Mallet TMR (trimedlure [TML], methyl eugenol [ME], raspberry ketone [RK]) wafers impregnated with DDVP (2, 2-dichlorovinyl dimethyl phosphate) insecticide were weathered during summer (8 wk) and winter (12 wk) in five California citrus-growing counties (Kern, Ventura, Orange, Tulare, and Riverside). In addition, TMR wafers without DDVP and with a Hercon Vaportape II insecticidal strip were compared with TMR dispensers with DDVP at Exeter and Riverside. Weathered treatments were shipped every week (overnight delivery) to Hawaii and frozen for a later bioassay in a 1,335-ha coffee plantation near Numila, Kauai Island, HI, where Mediterranean fruit fly, Ceratitis capitata (Wiedemann), oriental fruit fly, Bactrocera dorsalis Hendel, and melon fly, Bactrocera cucurbitae Coquillett, were all present. We compared trap captures of the three species, C. capitata, B. dorsalis, and B. cucurbitae, for the five different weathering locations. Captures of C. capitata, B. dorsalis, and B. cucurbitae with Mallet TMR dispensers (with DDVP) were not significantly different for the five locations. Captures with the Mallet TMR dispenser without DDVP and Vaportape were similar to those for Mallet TMR with DDVP, although there were some slight location differences. In conclusion, based on these results, the Mallet TMR dispenser could potentially be used in California habitats where large numbers of detection traps are currently deployed. Use of Vaportape with dispensers would not require them to be registered with US Environmental Protection Agency (EPA). Dispensers for use as Male Annihilation Technique (MAT) devices will be tested further in Hawaii.

  7. Assessment of attractiveness of plants as roosting sites for the melon fly, Bactrocera cucurbitae, and oriental fruit fly, Bactrocera dorsalis.

    Science.gov (United States)

    McQuate, Grant T; Vargas, Roger I

    2007-01-01

    The use of toxic protein bait sprays to suppress melon fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae), populations typically involves application to vegetation bordering agricultural host areas where the adults seek shelter ("roost"). Although bait spray applications for suppression of oriental fruit fly, Bactrocera dorsalis (Hendel), populations have traditionally been applied to the host crop, rather than to crop borders, roosting by oriental fruit flies in borders of some crop species, such as papaya, Carica papaya L. (Brassicales: Caricaceae), suggests that bait spray applications to crop borders could also help in suppression of B. dorsalis populations. In order to develop improved recommendations for application of bait sprays to border plants for suppression of melon fly and oriental fruit fly populations, the relative attractiveness of a range of plant species, in a vegetative (non-flowering) stage, was tested to wild melon fly and oriental fruit fly populations established in a papaya orchard in Hawaii. A total of 20 plant species were evaluated, divided into four categories: 1) border plants, including corn, Zea mays L. (Poales: Poaceae), windbreaks and broad-leaved ornamentals, 7 species; 2) weed plants commonly found in agricultural fields in Hawaii, 6 species; 3) host crop plants, 1 species- zucchini, Cucurbita pepo L. (Violales: Curcurbitaceae), and 4) locally grown fruit trees, 6 species. Plants were established in pots and placed in an open field, in clusters encircling protein bait traps, 20 m away from the papaya orchard. Castor bean, Ricinus communis L. (Euphorbiales: Euphorbiaceae), panax, Polyscias guilfoylei (Bull) Bailey (Apiales: Araliaceae), tiger's claw, Erythnna variegata L. (Fabales: Fabaceae), and guava, Psidium guajava L. (Myrtales: Myrtaceae) were identified as preferred roosting hosts for the melon fly, and tiger's claw, panax, castor bean, Canada cocklebur, Xanthium strumarium L. (Asterales: Asteraceae), Brazilian

  8. Effect of Cucurbita ficifolia and Probiotic Yogurt Consumption on Blood Glucose, Lipid Profile, and Inflammatory Marker in Type 2 Diabetes

    Science.gov (United States)

    Bayat, Azade; Azizi-Soleiman, Fatemeh; Heidari-Beni, Motahar; Feizi, Awat; Iraj, Bijan; Ghiasvand, Reza; Askari, Gholamreza

    2016-01-01

    Background: Control of blood sugar, hypertension, and dyslipidemia are key factors in diabetes management. Cucurbita ficifolia (pumpkin) is a vegetable which has been used traditionally as a remedy for diabetes in Iran. In addition, consumption of probiotics may have beneficial effects on people with Type 2 diabetes. The aim of this study was an investigation of the effects of C. ficifolia and probiotic yogurt consumption alone or at the same time on blood glucose and serum lipids in diabetic patients. Methods: Eighty eligible participants randomly were assigned to four groups: 1 - green C. ficifolia (100 g); 2 - probiotic yogurt (150 g); 3 - C. ficifolia plus probiotic yogurt (100 g C. ficifolia plus 150 g yogurt); and 4 -control (dietary advice) for 8 weeks. Blood pressure, glycemic response, lipid profile, and high-sensitive C-reactive protein (hsCRP) were measured before and after the intervention. Results: Total cholesterol (TC) decreased significantly in yogurt and yogurt plus C. ficifolia groups (within groups P = 0.010, and P < 0.001, respectively). C. ficifolia plus yogurt consumption resulted in a decrease in triglyceride (TG) and an increase in high-density lipoprotein cholesterol (HDL-C) (within groups P < 0.001 and P = 0.001, respectively). All interventions led to a significant decrease in blood sugar, hemoglobin A1c (HbA1c), hsCRP, and low-density lipoprotein cholesterol (LDL-C) level within groups. Blood pressure decreased significantly in Cucurbita group and yogurt group (within groups P < 0.001, and P = 0.001 for systolic blood pressure [SBP] and P < 0.001, and P = 0.004 for diastolic blood pressure [DBP], respectively). All variables changed between groups significantly except LDL-C level. Conclusions: Variables including TG, HDL-C, TC, fasting blood sugar, HbA1c, SBP, DBP, and hsCRP changed beneficially between groups. It seems that consumption of C. ficifolia and probiotic yogurt may help treatment of diabetic patients. PMID:26955460

  9. 南瓜果肉可溶性固形物含量与叶、花、果肉色泽的相关性分析%Correlation analysis between the soluble solids content of fruit sarcocarp and colour of leaf, flower, fruit sarcocarp on Cucurbita moschata

    Institute of Scientific and Technical Information of China (English)

    周俊国; 鲁晓晓; 杨道印; 聂建满

    2016-01-01

    The colour values of leaf, flower, fruit sarcocarp by CR-3 colorimeter and the soluble solids content (SSC) of fruit sarcocarp by the PAL-1 digital display sugar meter were measured for 14 pumpkin cultivars respectively in this experiment. The correlation between colour values of leaf, flower, fruit sarcocarp and SSC of fruit sarcocarp were analyzed. The results showed that there were differences between the coefficient of variation of leaf, flower, fruit sarcocarp colour values, and fruit sarcocarp redness in the coefficient of variation was the largest (CV=27.43%). SSC of fruit sarcocarp was highly significantly positive correlated (P<0.01, r=0.632 ) with brightness of leaf, and it was significantly positive correlated (P<0.05, r=0.412 ) with its redness. This Results provided a convenient method to screen high SSC of fruit sarcocarp in the filed for pumpkin quality breeding.%以14种不同品种的南瓜为材料,在果实成熟期采用CR-3型色差仪测量其叶片、花朵、果肉的Lab值,同时采用PAL-1型测糖仪测量果肉中可溶性固形物含量,并对叶、花、果肉的Lab值与果肉中可溶性固形物含量进行了相关性分析。结果表明,南瓜叶、花、果肉色泽的Lab值的变异系数有差异,果肉中红色成分变异系数最大,达27.43%;果肉中可溶性固形物含量与叶片的亮度呈极显著正相关(r=0.632),与果肉中红色成分呈显著正相关(r=0.412)。这为南瓜品质育种田间筛选可溶性固形物含量高的试验材料提供了便捷的方法。

  10. Analysis of the volatile constituents from seeds of chaotianmiben Cucurbita moschata Duch.by head-space solid micro-extraction,coupled with GC-MS%HS-SPME/GC-MS 分析超甜蜜本南瓜籽挥发性成分

    Institute of Scientific and Technical Information of China (English)

    李昌勤; 王海燕; 卢引; 薛志平; 康文艺

    2013-01-01

    目的 分析超甜蜜本南瓜籽挥发性成分.方法 采用顶空固相微萃取和气质联用技术(HS-SPME/GC-MS )结合保留指数法,并用峰面积归一化法测定相对百分含量.结果 从超甜蜜本南瓜籽中鉴定出35个化合物,占总峰面积的96.34%.结论 棕榈酸乙酯含量最高,占总成分的24.52%,其次为[R,R]-2,3-丁二醇(14.30%)、亚油酸乙酯(11.67%).

  11. Analysis of volatile components from the pulp of chaotianmiben Cucurbita moschata Duch.by head-space solid phase micro-extraction coupled with GC-MS%顶空固相微萃取法分析超甜蜜本南瓜肉挥发性成分

    Institute of Scientific and Technical Information of China (English)

    李昌勤; 王海燕; 卢引; 康文艺

    2013-01-01

    目的 分析超甜蜜本南瓜肉挥发性成分.方法 采用顶空固相微萃取和气质联用技术(HS-SPME/GC-MS ) 结合保留指数法,首次并用峰面积归一化法测定相对百分含量.结果 从超甜蜜本南瓜的肉中鉴定出22个化合物,占总峰面积的92.59%.结论 研究表明其挥发性成分中吡喃酮含量最高(14.87%),其次为棕榈酸 (13.20%)等.

  12. HS-SPME/GC-MS 分析超甜蜜本南瓜雄花挥发性成分%Analysis of volatile components from the male flowers of chaotianmiben Cucurbita moschata Duch.by head-space solid phase micro-extraction coupled with GC-MS

    Institute of Scientific and Technical Information of China (English)

    卢引; 李昌勤; 李新铮; 邢晗; 康文艺

    2013-01-01

    目的 分析超甜蜜本南瓜雄花挥发性成分.方法 采用顶空固相微萃取和气质联用技术(HS-SPME/GC-MS )结合保留指数法,首次并用峰面积归一化法测定相对百分含量.结果 从超甜蜜本南瓜的雄花中鉴定出26个化合物,占总峰面积的95.66%.结论 研究表明β-波旁烯含量最高(21.68%),其次为香叶烯 D(9.80%)、二甲基硫醚(9.16%)等.

  13. Gama-Tocopherol Accumulation and Floral Differentiation of Medicinal Pumpkin (Cucurbita pepo L. in Response to Plant Growth Regulators

    Directory of Open Access Journals (Sweden)

    Mohammad SEDGHI

    2008-08-01

    Full Text Available Changes in biochemical and agronomical characteristics were studied in medicinal pumpkin (Cucurbita pepo L. plants under different treatments with plant growth regulators (PGRs. The seeds were subjected to priming with PGRs before planting and plants unerwent foliar sprays with different solutions of PGRs at 10 day-intervals after flowering. y-tocopherol (y-toc content of grains was found to be increased under gibberellic acid (GA3 treatment. The accumulation of y-tocopherol in the grains of GA3 treated plants was approximately 19.5 % higher than the control and reached 220.2 mg kg-1 dry pumpkin grains. The number of female flowers per plant was positively affected by PGRs and was 10.1 and 1.89 respectively, in the naphthalene acetic acid (NAA and GA3 treated plants. A significant efficiency of treatments was observed upon fresh fruit yield. The yield increased from 4831 t ha-1 in the control to 6820 t ha-1 with NAA treatment. Priming treatments increased seedling emergence rate and percent. The highest seedling emergence rate was found in GA3 treated seeds.

  14. Changes in the ascorbate system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress.

    Science.gov (United States)

    Dipierro, Nunzio; Mondelli, Donato; Paciolla, Costantino; Brunetti, Gennaro; Dipierro, Silvio

    2005-05-01

    The involvement of the ascorbate (AsA) system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress was studied. The treatment of 5-day-old pumpkin seedlings with 50 microM aluminium sulphate resulted in approximately 60% inhibition of root growth within 48-60 h of treatment, while aluminium accumulated in the roots reaching a maximum within 48h. During the same period, the hydrogen peroxide content of the roots was strongly enhanced. The increased level of hydrogen peroxide was matched by both increased ascorbate peroxidase (APX) (EC 1.11.1.11) activity and ascorbate free radical reductase (AFRR) (EC 1.1.5.4) activity, while dehydroascorbate reductase (DHAR) (EC 1.8.5.1) and glutathione reductase (GR) (EC 1.6.4.2) did not change. The levels of AsA in the roots were also increased by the Al treatment. It was concluded that an oxidative burst is probably involved in the toxicity of Al in pumpkin roots and that plants react to the enhanced production of reactive oxygen species by expressing higher levels of scavenging systems such as the AsA-APX system.

  15. Physiological effects of magnetite (Fe3O4) nanoparticles on perennial ryegrass (Lolium perenne L.) and pumpkin (Cucurbita mixta) plants.

    Science.gov (United States)

    Wang, Huanhua; Kou, Xiaoming; Pei, Zhiguo; Xiao, John Q; Shan, Xiaoquan; Xing, Baoshan

    2011-03-01

    To date, knowledge gaps and associated uncertainties remain unaddressed on the effects of nanoparticles (NPs) on plants. This study was focused on revealing some of the physiological effects of magnetite (Fe(3)O(4)) NPs on perennial ryegrass (Lolium perenne L.) and pumpkin (Cucurbita mixta cv. white cushaw) plants under hydroponic conditions. This study for the first time reports that Fe(3)O(4) NPs often induced more oxidative stress than Fe(3)O(4) bulk particles in the ryegrass and pumpkin roots and shoots as indicated by significantly increased: (i) superoxide dismutase and catalase enzyme activities, and (ii) lipid peroxidation. However, tested Fe(3)O(4) NPs appear unable to be translocated in the ryegrass and pumpkin plants. This was supported by the following data: (i) No magnetization was detected in the shoots of either plant treated with 30, 100 and 500 mg l(-1) Fe(3)O(4) NPs; (ii) Fe K-edge X-ray absorption spectroscopic study confirmed that the coordination environment of Fe in these plant shoots was similar to that of Fe-citrate complexes, but not to that of Fe(3)O(4) NPs; and (iii) total Fe content in the ryegrass and pumpkin shoots treated with Fe(3)O(4) NPs was not significantly increased compared to that in the control shoots.

  16. The effects of pruning and nodal adventitious roots on polychlorinated biphenyl uptake by Cucurbita pepo grown in field conditions

    Energy Technology Data Exchange (ETDEWEB)

    Low, Jennifer E.; Whitfield Aslund, Melissa L. [Department of Chemistry and Chemical Engineering, Royal Military College of Canada, PO Box 17000 Station Forces, Kingston, ON, K7K 7B4 (Canada); Rutter, Allison [School of Environmental Studies, Rm 0626 Biosciences Complex, Queen' s University, 116 Barrie St., Kingston, ON, K7L 3N6 (Canada); Zeeb, Barbara A., E-mail: zeeb-b@rmc.ca [Department of Chemistry and Chemical Engineering, Royal Military College of Canada, PO Box 17000 Station Forces, Kingston, ON, K7K 7B4 (Canada)

    2011-03-15

    Two cultivation techniques (i-pruning and ii-nodal adventitious root encouragement) were investigated for their ability to increase PCB phytoextraction by Cucurbita pepo ssp pepo cv. Howden (pumpkin) plants in situ at a contaminated industrial site in Ontario (Aroclor 1248, mean soil [PCB] = 5.6 {mu}g g{sup -1}). Pruning was implemented to increase plant biomass close to the root where PCB concentration is known to be highest. This treatment was found to have no effect on final shoot biomass or PCB concentration. However, material pruned from the plant is not included in the final shoot biomass. The encouragement of nodal adventitious roots at stem nodes did significantly increase the PCB concentration in the primary stem, while not affecting shoot biomass. Both techniques are easily applied cultivation practices that may be implemented to decrease phytoextraction treatment time. - Research highlights: > Presence of nodal adventitious roots do increase phytoextraction efficiency. > Pruning may increase the biomass of pumpkin plants during phytoextraction. > [Aroclor 1248] decreases in plant tissue with increasing distance from the root. - The application of cultivation practices (pruning and nodal adventitious root encouragement) increases phytoextraction of PCBs in C. pepo.

  17. Tryptophan exposure and accessibility in the chitooligosaccharide-specific phloem exudate lectin from pumpkin (Cucurbita maxima). A fluorescence study.

    Science.gov (United States)

    Narahari, Akkaladevi; Swamy, Musti J

    2009-10-06

    The exposure and accessibility of the tryptophan residues in the chitooligosaccharide-specific pumpkin (Cucurbita maxima) phloem exudate lectin (PPL) have been investigated by fluorescence spectroscopy. The emission lambda(max) of native PPL, seen at 338nm was red-shifted to 348nm upon denaturation by 6M Gdn.HCl in the presence of 10mM beta-mercaptoethanol, indicating near complete exposure of the tryptophan residues to the aqueous medium, whereas a blue-shift to 335nm was observed in the presence of saturating concentrations of chitotriose, suggesting that ligand binding leads to a decrease in the solvent exposure of the tryptophan residues. The extent of quenching was maximum with the neutral molecule, acrylamide whereas the ionic species, iodide and Cs(+) led to significantly lower quenching, which could be attributed to the presence of charged amino acid residues in close proximity to some of the tryptophan residues. The Stern-Volmer plot for acrylamide was linear for native PPL and upon ligand binding, but became upward curving upon denaturation, indicating that the quenching occurs via a combination of static and dynamic mechanisms. In time-resolved fluorescence experiments, the decay curves could be best fit to biexponential patterns, for native protein, in the presence of ligand and upon denaturation. In each case both lifetimes systematically decreased with increasing acrylamide concentrations, indicating that quenching occurs predominantly via a dynamic process.

  18. Genetic relationships and evolution in Cucurbita pepo (pumpkin, squash, gourd) as revealed by simple sequence repeat polymorphisms.

    Science.gov (United States)

    Gong, Li; Paris, Harry S; Nee, Michael H; Stift, Gertraud; Pachner, Martin; Vollmann, Johann; Lelley, Tamas

    2012-03-01

    Genetic relationships among 104 accessions of Cucurbita pepo were assessed from polymorphisms in 134 SSR (microsatellite) and four SCAR loci, yielding a total of 418 alleles, distributed among all 20 linkage groups. Genetic distance values were calculated, a dendrogram constructed, and principal coordinate analyses conducted. The results showed 100 of the accessions as distributed among three clusters representing each of the recognized subspecies, pepo, texana, and fraterna. The remaining four accessions, all having very small, round, striped fruits, assumed central positions between the two cultivated subspecies, pepo and texana, suggesting that they are relicts of undescribed wild ancestors of the two domesticated subspecies. In both, subsp. texana and subsp. pepo, accessions belonging to the same cultivar-group (fruit shape) associated with one another. Within subsp. pepo, accessions grown for their seeds or that are generalists, used for both seed and fruit consumption, assumed central positions. Specialized accessions, grown exclusively for consumption of their young fruits, or their mature fruit flesh, or seed oil extraction, tended to assume outlying positions, and the different specializations radiated outward from the center in different directions. Accessions of the longest-fruited cultivar-group, Cocozelle, radiated bidirectionally, indicating independent selection events for long fruits in subsp. pepo probably driven by a common desire to consume the young fruits. Among the accessions tested, there was no evidence for crossing between subspecies after domestication.

  19. A biosensor based on zucchini (Cucurbita pepo L.) homogenate as a biorecognition layer for ascorbic acid determination.

    Science.gov (United States)

    Sezgintürk, Mustafa Kemal; Koca, Halit Buğra; Ozben, Yavuz Selim; Dinçkaya, Erhan

    2010-08-01

    An amperometric biosensor based on zucchini (Cucurbita pepo) tissue homogenate is presented. The zucchini tissue homogenate was crosslinked with gelatine using glutaraldehyde and fixed on a pretreated teflon membrane. The zucchini tissue contained the enzyme ascorbate oxidase and this enzyme catalyzed the oxidation of ascorbic acid in the presence of dissolved oxygen. The principle of the measurements was based on the determination of the decrease in the dissolved oxygen level. Determinations were carried out by standard curves, which were obtained by the measurement of the decrease in the oxygen level related to ascorbic acid concentration. Optimization and characterization studies of the biosensor were carried out in detail. First of all, the amounts of zucchini tissue homogenate, gelatin, and glutaraldehyde percentage were optimized. Experimental parameters such as buffer system, pH, buffer concentration, and temperature were also optimized carefully. Thermal stability, storage stability, and repeatability of the biosensor were investigated. A linear response was observed from 5x10(-6) M to 1.2x10(-3) M ascorbic acid. Finally, the results of some plant and drug samples analyzed with the presented biosensor compared with the spectrophotometric method (Tillman reagent) used as a reference.

  20. Bacterial degradation of Aroclor 1242 in the mycorrhizosphere soils of zucchini (Cucurbita pepo L.) inoculated with arbuscular mycorrhizal fungi.

    Science.gov (United States)

    Qin, Hua; Brookes, Philip C; Xu, Jianming; Feng, Youzhi

    2014-11-01

    A greenhouse experiment was conducted to investigate the effects of zucchini (Cucurbita pepo L.), inoculated with the arbuscular mycorrhizal (AM) species Acaulospora laevis, Glomus caledonium, and Glomus mosseae, on the soil bacterial community responsible for Aroclor 1242 dissipation. The dissipation rates of Aroclor 1242 and soil bacteria abundance were much higher with the A. laevis and G. mosseae treatments compared to the non-mycorrhizal control. The biphenyl dioxygenase (bphA) and Rhodococcus-like 2,3-dihydroxybiphenyl dioxygenase (bphC) genes were more abundant in AM inoculated soils, suggesting that the bphA and Rhodococcus-like bphC pathways play an important role in Aroclor 1242 dissipation in the mycorrhizosphere. The soil bacterial communities were dominated by classes Betaproteobacteria and Actinobacteria, while the relative proportion of Actinobacteria was significantly (F=2.288, P<0.05) correlated with the PCB congener profile in bulk soil. Our results showed that AM fungi could enhance PCB dissipation by stimulating bph gene abundance and the growth of specific bacterial groups.

  1. Effect of pistil age on pollen tube growth, fruit development and seed set in Cucurbita pepo L.

    Directory of Open Access Journals (Sweden)

    Ettore Pacini

    2014-01-01

    Full Text Available The effects of pistil age on pollen tube growth, fruit development and seed set were studied in Cucurbita pepo L., the flower of which opens for only six hours. Stigma receptivity lasts four days, from one day before until two days after anthesis. Style receptivity lasts three days, from the day before to the day after anthesis. Ovule receptivity lasts two days: the day of anthesis and the day before. The rate of pollen tube growth varies in different parts of the pistil and in relation to pistil age. In the stigmatic and stylar region, the tubes grow faster if pollination occurs the day before anthesis; in the ovary they grow faster when pollination occurs at anthesis. In the receptacle region, where the transmitting tissue is reduced, the growth rate decreases independently of the time of pollination. The fruits are larger and heavier with more seeds when pollination occurs at anthesis. There is a positive correlation between seed number and fruit weight when pollination occurred at anthesis and the day before.

  2. Activity of β-Galactosidase and Polygalacturonase in Zucchini Squash (Cucurbita pepo L. Stored at Low Temperatures

    Directory of Open Access Journals (Sweden)

    René Renato Balandrán-Quintana

    2007-01-01

    Full Text Available When fruits are exposed to chilling temperatures, enzymatic systems are affected and normal cell metabolism is altered; cell wall enzymes are the least studied in this respect. Our objective is to determine the effect of storage temperature and/or kind of tissue on the activity of the enzymes polygalacturonase (PG and β-galactosidase (β-gal in zucchini squash (Cucurbita pepo L. subjected to 2.5 and/or 12 °C for 16 days. Exocarp and endocarp samples were analyzed every 4 days for PG and β-gal activities. The highest β-gal activity was found in the exocarp at 12 °C; in both tissues β-gal decreased with time at 2.5 °C, but increased at 12 °C. PG activity was higher in the endocarp (highest activity at 2.5 °C than in the exocarp (highest activity at 12 °C. It was concluded that PG activity in the exocarp constitutes an adequate system for studying the relationship between chilling injury and cell wall biological activity.

  3. Implementing a spinosad-based local bait station to control Bactrocera cucurbitae (Diptera: Tephritidae) in high rainfall areas of Reunion Island.

    Science.gov (United States)

    Delpoux, Camille; Deguine, Jean-Philippe

    2015-01-01

    Three species of fruit flies cause serious damage to cucurbit crops on Reunion Island: Bactrocera cucurbitae (Diptera: Tephritidae) (Coquillett 1899), Dacus ciliatus (Loew 1901), and Dacus demmerezi (Bezzi 1917). To control them, a program of agroecological management of cucurbit flies has been implemented based on the application of Synéis-appât, especially spot sprays on corn borders. However, the high rainfall on Reunion Island limits the long-term efficiency of the bait; in addition, this method cannot be used for large chayote trellises, because corn borders cannot be planted around them. The aim of this study was to design a bait station adapted to prevailing conditions on Reunion Island. An 'umbrella trap' tested in Taiwan was used as a reference to compare its efficacy with our local bait station. Experiments were conducted in field cages on B. cucurbitae to test different characteristics of bait stations and to construct one using local materials. Results were validated in the field. The attractiveness of the bait station was related mainly to the color of the external surface, yellow being the most attractive color. The efficacy of the bait station with respect to fly mortality was found to be linked to the accessibility of the bait, and direct application of Synéis-appât on the bait station was found to be the most efficient. In the field, B. cucurbitae were more attracted to the local bait station than to the umbrella trap, while the two other fly species displayed equal attraction to both trap types. Our local bait station is a useful alternative to spot sprays of Synéis-appât and is now included in a local pest management program and is well accepted by farmers.

  4. Weathering and Chemical Degradation of Methyl Eugenol and Raspberry Ketone Solid Dispensers for Detection, Monitoring, and Male Annihilation of Bactrocera dorsalis and Bactrocera cucurbitae (Diptera: Tephritidae) in Hawaii.

    Science.gov (United States)

    Vargas, Roger I; Souder, Steven K; Nkomo, Eddie; Cook, Peter J; Mackey, Bruce; Stark, John D

    2015-08-01

    Solid male lure dispensers containing methyl eugenol (ME) and raspberry ketone (RK), or mixtures of the lures (ME + RK), and dimethyl dichloro-vinyl phosphate (DDVP) were evaluated in area-wide pest management bucket or Jackson traps in commercial papaya (Carica papaya L.) orchards where both oriental fruit fly, Bactrocera dorsalis (Hendel), and melon fly, Bactrocera cucurbitae (Coquillett), are pests. Captures of B. dorsalis with fresh wafers in Jackson and bucket traps were significantly higher on the basis of ME concentration (Mallet ME [56%] > Mallet MR [31.2%] > Mallet MC [23.1%]). Captures of B. cucurbitae with fresh wafers in Jackson and bucket traps were not different regardless of concentration of RK (Mallet BR [20.1%] = Mallet MR [18.3%] = Mallet MC [15.9%]). Captures of B. dorsalis with fresh wafers, compared with weathered wafers, were significantly different after week 12; captures of B. cucurbitae were not significantly different after 16 wk. Chemical analyses revealed presence of RK in dispensers in constant amounts throughout the 16-wk trial. Degradation of both ME and DDVP over time was predicted with a high level of confidence by nonlinear asymptotic exponential decay curves. Results provide supportive data to deploy solid ME and RK wafers (with DDVP) in fruit fly traps for detection programs, as is the current practice with solid TML dispensers placed in Jackson traps. Wafers with ME and RK might be used in place of two separate traps for detection of both ME and RK responding fruit flies and could potentially reduce cost of materials and labor by 50%.

  5. Produção de variedades de Cucurbita pepo premunizadas com estirpes fracas do Papaya ringspot virus - type W e do Zucchini yellow mosaic virus

    OpenAIRE

    Estela Bonilha; Ricardo Gioria; Rômulo Fujito Kobori; Paulo Tarcísio Della Vecchia; Sônia Maria de Stefano Piedade; Jorge Alberto Marques Rezende

    2009-01-01

    Papaya ringspot virus - type W (PRSV-W) and Zucchini yellow mosaic virus (ZYMV) are the most prevalent viruses in cucurbit crops in Brazil and responsible for frequent yield losses. Diseases caused by these viruses are difficult to control. The objective of this work was to evaluate the effects of mild strains PRSV-W-1 and ZYMV-M on the yield of Cucurbita pepo L. cvs. Samira, Novita Plus, AF 2847, and Yasmin, under plastic greenhouse and field conditions. Plants infected with ZYMV-M and grown...

  6. A novel 3α-p-Nitrobenzoylmultiflora-7:9(11)-diene-29-benzoate and two new triterpenoids from the seeds of zucchini (Cucurbita pepo L).

    Science.gov (United States)

    Tanaka, Reiko; Kikuchi, Takashi; Nakasuji, Saori; Ue, Yasuhiro; Shuto, Daisuke; Igarashi, Keishi; Okada, Rina; Yamada, Takeshi

    2013-06-26

    Three novel multiflorane-type triterpenoids, 3α-p-nitrobenzoylmultiflora-7:9(11)-diene-29-benzoate (1), 3α-acetoxymultiflora-7:9(11)-diene-29-benzoate (2), and 3α-acetoxymultiflora-5(6):7:9(11)-triene-29-benzoate (3), along with two known related compounds 4 and 5 were isolated from the seeds of zucchini (Cucurbita pepo L). Their structures were determined on the basis of 1D and 2D NMR spectroscopy and HREIMS. Triterpenoids possessing a nitro group were not isolated previously.

  7. Nutrientes e propriedades funcionais em sementes de abóbora (Cucurbita maxima submetidas a diferentes processamentos Nutrients and functional properties in pumpkin seed (Cucurbita maxima submitted to different processings

    Directory of Open Access Journals (Sweden)

    Luciana de Paula Naves

    2010-05-01

    Full Text Available O objetivo deste trabalho foi verificar a influência dos processamentos térmicos sobre os nutrientes e propriedades funcionais das sementes da abóbora Cucurbita maxima. As sementes foram, em quatro repetições, submetidas aos seguintes processamentos: utilizadas na forma crua; cozidas em água em ebulição por três tempos: 5, 10 e 15 minutos; e cozidas no vapor por 10 minutos. Posteriormente foram liofilizadas, trituradas e armazenadas em temperatura ambiente até a realização das análises de composição centesimal, minerais e propriedades funcionais. Não houve diferença significativa entre os processamentos para os níveis de proteína bruta, fibra alimentar, extrato etéreo, cinzas, S, P, Mg, Ca, Cu, Zn, solubilidade do nitrogênio (nos pH 4, 5 e 6, absorção de água e óleo, volume de espuma e estabilidade de emulsão. O cozimento em água em ebulição reduziu o teor de K. Todos os processamentos térmicos diminuíram os níveis de Mn e Fe. As sementes cruas apresentaram a maior solubilidade do nitrogênio nos pH 2, 3, 7, 8 e 9. Conclui-se que os processamentos acarretaram diferença significativa apenas nos teores de K, Mn, Fe e solubilidade do nitrogênio; e que as sementes apresentam potencial para serem incorporadas, provavelmente, em alimentos que requeiram elevada taxa de absorção de óleo.The objective of this paper was to verify the influence of the thermal processings on the nutrients and functional properties of the pumpkin seeds Cucurbita maxima. Seeds were, in four repetitions, submitted to the following processings: used in the raw form; cooked in boiling water for three times: 5, 10 and 15 minutes; and cooked in steam for 10 minutes. Thereafter were freeze-dried, grinded and stored at room temperature up to accomplishment of the analyses of centesimal composition, minerals and functional properties. There wasn't significant difference among the processings to the levels of crude protein, dietary fiber, ether

  8. 中国南瓜主要性状遗传特性的研究%Study on Genetic Character of Cultivars moschata Main Traits

    Institute of Scientific and Technical Information of China (English)

    李新峥; 杨鹏鸣; 刘振威; 杜晓华

    2011-01-01

    Genetic laws, correlation, heterosis of main traits of Cultivars moschata, Chinese pumpkin, were studied by using twenty-four representative inbred lines and hybrids crossed by the inbreds. The results showed that genetic laws of Chinese pumpkin were complex. In same trait, some Chinese pumpkins behaved qualitative character while other Chinese pumpkins behaved quantitative character. Variation coefficients of Chinese pumpkin 7 traits in turn were as followings: fruit shape index, powdery mildew disease index, viral disease index, mass of single fruit, yield, soluble solid content and first fruit node. Yield and powdery mildew disease index of Chinese pumpkin had high heterosis from mid-parent and soluble solid content had low heterosis from mid-parent. There were significant correlations between fruit shape index and first fruit node, yield and mass of single fruit. There were significant negative correlations between fruit shape index and mass of single fruit, yield and viral disease index, viral disease index and soluble solid content. Chinese pumpkins resisted viral disease was 84. 4% while Chinese pumpkins sensed powdery mildew disease was 81.3%.%选择具有代表性的24个中国南瓜自交系和由这些自交系所组配的杂交组合作样本,对其主要农艺性状的遗传规律、相关性及杂种优势进行分析.结果表明:南瓜主要性状遗传规律较为复杂,同一性状,有些南瓜表现为质量性状,有些南瓜表现为数量性状.中国南瓜7种性状的变异系数大小依次为果形指数、白粉病病情指数、病毒病病情指数、单果质量、产量、可溶性同形物含量、第1坐果节位.产量和白粉病病情指数具有较明显的离中优势,可溶性同形物的离中优辨较低.果形指数与第1坐果节位呈极显著正相关,与单果质量呈极显著负相关.产量与单果质量呈极显著正相关,与病毒病情指数呈极显著负相关.病毒病病情指数与南瓜果实可溶

  9. Mutational analysis of the pumpkin (Cucurbita maxima) phloem exudate lectin, PP2 reveals Ser-104 is crucial for carbohydrate binding.

    Science.gov (United States)

    Bobbili, Kishore Babu; Bandari, Shyam; Grobe, Kay; Swamy, Musti J

    2014-07-18

    The pumpkin phloem lectin (PP2) is an RNA-binding, defense-related, chitooligosaccharide-specific, homodimeric lectin of Mr 48 kDa expressed at high concentrations in the sieve elements and companion cells of pumpkin (Cucurbita maxima). In the present study, PP2 was expressed in the methylotrophic yeast Pichia pastoris with the Saccharomyces α-factor sequence to direct the recombinant protein into the secretory pathway as a prerequisite for unimpaired folding and posttranslational glycosylation of recombinant PP2. Previous computational modeling and ligand docking studies predicted a putative chitooligosaccharide-binding site on the PP2 surface, which was divided into three subsites, with two amino acid residues in each subsite identified as possible candidates for interaction with chitooligosaccharides (CHOs). In this work, mutational analysis and hemagglutination assays were employed to verify the role of the predicted residues in the carbohydrate binding activity of the protein. The results obtained revealed that mutation of Ser-104 to Ala (S104A) at subsite-2 resulted in about 90% loss of agglutination activity of the protein, indicating that Ser-104 is crucial for the binding of CHOs to PP2. Also, L100A (at subsite-1) and K200A (at subsite-3) independently decreased the lectin activity by about 40%, indicating that these two residues also contribute significantly to sugar binding by PP2. Together, these findings confirm that all the three subsites contribute to varying degrees toward PP2-carbohydrate interaction, and confirm the validity of the computational model, as proposed earlier.

  10. Oligoglucans as Elicitors of an Enzymatic Antioxidant System in Zucchini Squash (Cucurbita pepo L. Seedlings at Low Temperature

    Directory of Open Access Journals (Sweden)

    Abel Ceron-Garcia

    2011-01-01

    Full Text Available Problem statement: Non-controlled low temperatures affect physiological and biochemical processes leading to Reactive Oxygen Species (ROS accumulation in plants, which normally are stabilized by the antioxidant system. Superoxide Dismutase (SOD, Ascorbate Peroxidase (APX and Catalase (CAT are the most important ROS-detoxification enzymes in stressed plants. Approach: The activity of these enzymes were examined in response to fungal Oligoglucans (OG in cold-stressed cotyledons of Cucurbita pepo L. Cold stress condition was settled in zucchini cotyledons using relative Ion Leakage (IL and H2O2 accumulation. Then, OG were obtained from Trichoderma harzianum cellwall by enzymatic and chemical hydrolysis. These elicitors were purified by size-exclusion and anionexchange chromatography and tested in cold-stressed cotyledons. Results: No significant changes on H2O2 content and IL were observed between treated and control cold-stressed cotyledons, during the first 7 days of storage at 5°C. Afterwards H2O2 values increased greatly after cold-stressed. Then, cold stress conditions were established (7 d, 5°C. The APX and CAT activities remained stable, but not SOD activity, which decreased about 42% in control cotyledons. When a chemical OG mixture (CM was applied to cold-stressed cotyledons, APX and CAT activity levels increased. The SOD activity was only increased by an enzymatic OG mixture (EM. The APX activity level was increased in coldstressed cotyledons using OG size-exclusion chromatography fractions from both CM and EM; however, changes in CAT activity were only possible using EM fractions and no changes were detected in SOD activity. The highest CAT activity levels were triggered using OG anion-exchange chromatography sub-fractions from both CM and EM fractions (120 and 119 % of enzymatic activity, respectively. Conclusion: The oxidative stress can be counteracted by the triggering of APX and CAT activity in cold

  11. Analysis of viral (zucchini yellow mosaic virus) genetic diversity during systemic movement through a Cucurbita pepo vine.

    Science.gov (United States)

    Dunham, J P; Simmons, H E; Holmes, E C; Stephenson, A G

    2014-10-13

    Determining the extent and structure of intra-host genetic diversity and the magnitude and impact of population bottlenecks is central to understanding the mechanisms of viral evolution. To determine the nature of viral evolution following systemic movement through a plant, we performed deep sequencing of 23 leaves that grew sequentially along a single Cucurbita pepo vine that was infected with zucchini yellow mosaic virus (ZYMV), and on a leaf that grew in on a side branch. Strikingly, of 112 genetic (i.e. sub-consensus) variants observed in the data set as a whole, only 22 were found in multiple leaves. Similarly, only three of the 13 variants present in the inoculating population were found in the subsequent leaves on the vine. Hence, it appears that systemic movement is characterized by sequential population bottlenecks, although not sufficient to reduce the population to a single virion as multiple variants were consistently transmitted between leaves. In addition, the number of variants within a leaf increases as a function of distance from the inoculated (source) leaf, suggesting that the circulating sap may serve as a continual source of virus. Notably, multiple mutational variants were observed in the cylindrical inclusion (CI) protein (known to be involved in both cell-to-cell and systemic movement of the virus) that were present in multiple (19/24) leaf samples. These mutations resulted in a conformational change, suggesting that they might confer a selective advantage in systemic movement within the vine. Overall, these data reveal that bottlenecks occur during systemic movement, that variants circulate in the phloem sap throughout the infection process, and that important conformational changes in CI protein may arise during individual infections. Copyright © 2014 Elsevier B.V. All rights reserved.

  12. Nutritional and anti-nutritional compositions of Cleome rutidosperma, Lagenaria siceraria, and Cucurbita maxima seeds from Nigeria.

    Science.gov (United States)

    Ojiako, O A; Igwe, C U

    2007-12-01

    Flours produced from the seeds of Cleome rutidosperma (DC), Lagenaria siceraria (Mol.), and Cucurbita maxima (Duch) were evaluated for proximate and elemental compositions. Preliminary phytochemical screening was also carried out on the seed flours, which revealed the presence of alkaloids, steroids, pentose, and reducing sugars in all the seed types. Tannins, flavonoids, and cardiac glycosides were also detected in only C. rutidosperma seed. The results also showed that the seeds of C. rutidosperma, L. siceraria, and C. maxima contained carbohydrates (74.43%, 45.93%, and 24.30%), fats (7.20%, 38.92%, and 51.49%), and proteins (11.73%, 8.93%, and 16.80%), respectively. The major mineral contents of the three defatted seed flours were found to include potassium (39.00 ppm, 19.50 ppm, and 39.00 ppm), sodium (23.00 ppm, 11.50 ppm, and 11.50 ppm), and calcium (18.00 ppm, 12.00 ppm, and 15.00 ppm), respectively. They were also found to contain zinc (0.013 ppm, 0.04 ppm, and 0.084 ppm) and iron (0.014 ppm, 0.028 ppm, and 0.032 ppm), respectively. The proximate and mineral compositions suggest that the seeds are potential sources of carbohydrate, fats, protein, and micro- and macrominerals and may find use as human food or for incorporation into livestock feed. However, the seeds were revealed to also contain slight traces of lead, although at such very low concentrations they may be artifacts. This calls for further research into possible lead toxicity after prolonged consumption of these seeds.

  13. Colonization and degradation of senescent flowers of zucchini squash by Trichoderma harzianum YC459, a biocontrol agent of gray mold, Botrytis cinerea

    Institute of Scientific and Technical Information of China (English)

    Geun Gon Kim; Young Ryun Chung

    2004-01-01

    @@ In commercial greenhouses, senescent flower petals or flowers of vegetables such as tomato,strawberry, hot pepper and zucchini squash were blighted to be removed from fruits within five days after spraying of Trichoderna harzianun YC459 (TORY() , JGreen Inc.), a biocontrol agent with good and consistent efficacy as chemical fungicides for the control of gray mold rot caused by B.cinerea. The mechanism for selective colonization of senescent floral tissues by T. harzianum YCA59was elucidated using fresh and senescent (4 days and 14 days after pollination, respectively) floral tissues of zucchini squash (Cucurbita moschata Duchesne) . The spores of T. harzianum YCA59were produced much more on water agar and liquid culture media supplemented with 5% dry powder of senescent floral tissues than with fresh tissues during 15 days incubation. Mycelial growth was also much better in the media with senescent tissues than with fresh tissues. Enzyme activities of carboxymethyl cellulase, amylase and polygalacturonase in the liquid media, which might be involved in the colonization and degradation of tissues by T. harzianum YCA59 were compared. The activities of three enzymes were significantly higher in the media with senescent floral tissues than with fresh floral tissues reaching to the maximum during 9 to 12 days of incubation. Especially, the activities of carboxymethyl cellulase and polygalacturonase of T. harzianum YC459 were much higher than those of other Trichoderma species, T. asperellum, T. viride and T. koningii in the liquid media with senescent floral tissues. Based on the results, the selective colonization and degradation of senescent floral tissues, an important habitat for B. cinerea, may be another mechanism for the biocontrol of gray mold rot of vegetables by T. harzianurm YC459.

  14. Buttercup squash provides a marketable alternative to blue hubbard as a trap crop for control of striped cucumber beetles (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Cavanagh, Andrew F; Adler, Lynn S; Hazzard, Ruth V

    2010-12-01

    Winter squash is a vital agricultural commodity worldwide. In the Northeastern United States, the primary insect pest is the striped cucumber beetle, Acalymma vittatum F. Using a Blue Hubbard squash (Cucurbita maxima Duchesne) perimeter trap crop system can reduce insecticide use by >90% in butternut squash (C. moschata Poir), the primary winter squash grown in this region. Despite the savings in insecticide costs, growers may be reluctant to give up field space for a perimeter crop of Blue Hubbard squash, which comprises only 5% of the winter squash market in New England as compared with 19% for buttercup squash. Finding a more marketable trap crop would lower the barrier for adoption of this system. We tested eight varieties of three species of cucurbits for attractiveness to beetles relative to Blue Hubbard and butternut squash, and chose buttercup squash as the most promising replacement. We compared the effect of a buttercup border, Blue Hubbard border, or control (no border) on beetle numbers, herbivory, insecticide use, pollination, and pollen limitation in the main crop. We found that buttercup squash performed equally well as Blue Hubbard as a trap crop, with 97% reduction in total insecticide use compared with control fields. Honey bees (Apis mellifera L.) and squash bees (Peponapis pruinosa Say) were the predominant pollinators, and border treatments did not affect visitation. Hand pollination did not increase reproduction or yield, indicating that natural pollination was sufficient for full yield. This study confirms the effectiveness of perimeter trap crop systems and offers growers a more marketable trap crop for managing cucumber beetle damage.

  15. Classification of pumpkin seed oils according to their species and genetic variety by attenuated total reflection Fourier-transform infrared spectroscopy.

    Science.gov (United States)

    Saucedo-Hernández, Yanelis; Lerma-García, María Jesús; Herrero-Martínez, José Manuel; Ramis-Ramos, Guillermo; Jorge-Rodríguez, Elisa; Simí-Alfonso, Ernesto F

    2011-04-27

    Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), followed by multivariate treatment of the spectral data, was used to classify seed oils of the genus Cucurbita (pumpkins) according to their species as C. maxima, C. pepo, and C. moschata. Also, C. moschata seed oils were classified according to their genetic variety as RG, Inivit C-88, and Inivit C-2000. Up to 23 wavelength regions were selected on the spectra, each region corresponding to a peak or shoulder. The normalized absorbance peak areas within these regions were used as predictors. Using linear discriminant analysis (LDA), an excellent resolution among all categories concerning both Cucurbita species and C. moschata varieties was achieved. The proposed method was straightforward and quick and can be easily implemented. Quality control of pumpkin seed oils is important because Cucurbita species and genetic variety are both related to the pharmaceutical properties of the oils.

  16. Phytoextraction of weathered p,p'-DDE by zucchini (Cucurbita pepo) and cucumber (Cucumis sativus) under different cultivation conditions.

    Science.gov (United States)

    Wang, Xiaoping; White, Jason C; Gent, Martin P N; Iannucci-Berger, William; Eitzer, Brian D; Mattina, MaryJane Incorvia

    2004-01-01

    Previous studies have shown that zucchini (Cucurbita pepo) and cucumber (Cucumis sativus) under field conditions are good and poor accumulators, respectively, of persistent organic pollutants from soil. Here, each species was grown under three cultivation regimes: dense (five plants in 5 kg soil): nondense (one plant in 80 kg soil): and field conditions (two to three plants in approximately 789 kg soil). p,p'-DDE and inorganic element content in roots, stems, leaves, and fruit were determined. In addition. rhizosphere, near-root, and unvegetated soil fractions were analyzed for concentrations of 11 low-molecular-weight organic acids (LMWOA) and 14 water-extractable inorganic elements. Under field conditions, zucchini phytoextracted 1.3% of the weathered p,p'-DDE with 98% of the contaminant in the aerial tissues. Conversely, cucumber removed 0.09% of the p,p'-DDE under field conditions with 83% in the aerial tissues. Under dense cultivation, cucumber produced a fine and fibrous root system not observed in our previous experiments and phytoextracted 0.78% of the contaminant, whereas zucchini removed only 0.59% under similar conditions. However. cucumber roots translocated only 5.7% of the pollutant to the shoot system, while in zucchini 48% of the p,p'-DDE in the plant was present in the aerial tissue. For each species, the concentrations of LMWOA in soil increased with increasing impact by the root system both within a given cultivation regime (i.e., rhizosphere > near-root > unvegetated) and across cultivation regimes (i.e., dense > nondense > field conditions). Under dense cultivation, the rhizosphere concentrations of LMWOAs were significantly greater for cucumber than for zucchini; no species differences were evident in the other two cultivation regimes. To enable direct comparison across cultivation regimes, total in planta p,p'-DDE and inorganic elements were mass normalized or multiplied by the ratio of plant mass to soil mass. For cucumber, differences in

  17. Risk assessment of genetically engineered crops: fitness effects of virus-resistance transgenes in wild Cucurbita pepo.

    Science.gov (United States)

    Laughlin, Karen D; Power, Alison G; Snow, Allison A; Spencer, Lawrence J

    2009-07-01

    The development of crops genetically engineered for pathogen resistance has raised concerns that crop-to-wild gene flow could release wild or weedy relatives from regulation by the pathogens targeted by the transgenes that confer resistance. Investigation of these risks has also raised questions about the impact of gene flow from conventional crops into wild plant populations. Viruses in natural plant populations can play important roles in plant fecundity and competitive interactions. Here, we show that virus-resistance transgenes and conventional crop genes can increase fecundity of wild plants under virus pressure. We asked how gene flow from a cultivated squash (Cucurbita pepo) engineered for virus resistance would affect the fecundity of wild squash (C. pepo) in the presence and absence of virus pressure. A transgenic squash cultivar was crossed and backcrossed with wild C. pepo from Arkansas. Wild C. pepo, transgenic backcross plants, and non-transgenic backcross plants were compared in field plots in Ithaca, New York, USA. The second and third generations of backcrosses (BC2 and BC3) were used in 2002 and 2003, respectively. One-half of the plants were inoculated with zucchini yellow mosaic virus (ZYMV), and one-half of the plants were maintained as healthy controls. Virus pressure dramatically decreased the fecundity of wild C. pepo plants and non-transgenic backcross plants relative to transgenic backcross plants, which showed continued functioning of the virus-resistance transgene. In 2002, non-transgenic backcross fecundity was slightly higher than wild C. pepo fecundity under virus pressure, indicating a possible benefit of conventional crop alleles, but they did not differ in 2003 when fecundity was lower in both groups. We detected no fitness costs of the transgene in the absence of the virus. If viruses play a role in the population dynamics of wild C. pepo, we predict that gene flow from transgenic, virus-resistant squash and, to a much lesser

  18. Italian horticultural and culinary records of summer squash (Cucurbita pepo, Cucurbitaceae) and emergence of the zucchini in 19th-century Milan.

    Science.gov (United States)

    Lust, Teresa A; Paris, Harry S

    2016-07-01

    Summer squash, the young fruits of Cucurbita pepo, are a common, high-value fruit vegetable. Of the summer squash, the zucchini, C. pepo subsp. pepo Zucchini Group, is by far the most cosmopolitan. The zucchini is easily distinguished from other summer squash by its uniformly cylindrical shape and intense colour. The zucchini is a relatively new cultivar-group of C. pepo, the earliest known evidence for its existence having been a description in a book on horticulture published in Milan in 1901. For this study, Italian-language books on agriculture and cookery dating from the 16th to 19th centuries have been collected and searched in an effort to follow the horticultural development and culinary use of young Cucurbita fruits in Italy. The results indicate that Cucurbita fruits, both young and mature, entered Italian kitchens by the mid-16th century. A half-century later, round and elongate young fruits of C. pepo were addressed as separate cookery items and the latter had largely replaced the centuries-old culinary use of young, elongate bottle gourds, Lagenaria siceraria Allusion to a particular, extant cultivar of the longest fruited C. pepo, the Cocozelle Group, dates to 1811 and derives from the environs of Naples. The Italian diminutive word zucchini arose by the beginning of the 19th century in Tuscany and referred to small, mature, desiccated bottle gourds used as containers to store tobacco. By the 1840s, the Tuscan word zucchini was appropriated to young, primarily elongate fruits of C. pepo The Zucchini Group traces its origins to the environs of Milan, perhaps as early as 1850. The word zucchini and the horticultural product zucchini arose contemporaneously but independently. The results confirm that the Zucchini Group is the youngest of the four cultivar-groups of C. pepo subsp. pepo but it emerged approximately a half-century earlier than previously known. © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany

  19. Differential responses in yield of pumpkin (Cucurbita maxima L.) and nightshade (Solanum retroflexum Dun.) to the application of three animal manures.

    Science.gov (United States)

    Azeez, J O; Van Averbeke, W; Okorogbona, A O M

    2010-04-01

    Crop responses to different manures differs considerably, however, the factors responsible for it have not been conclusively elucidated. Consequently, this study examined the biomass response of Cucurbita maxima and Solanum retroflexum to application rates of chicken and kraal manures of cattle and goat, and soil factors related to salinity. The crops' biomass yield increased linearly with increase in application rates of kraal and chicken manures, but steeper in the latter. Results showed that significant decline in biomass yield in chicken manure at rates above 8.5 tons ha(-1) were not due to salinity. The crops' response to cattle and goat kraal manures was linear but polynomial (cubic) in layer chicken manure. It was concluded that the yield decline in chicken manure was due to other manure factors except salinity, probably toxicity effect of the manure fatty acids. Further research was however, recommended to elucidate this claim.

  20. Effects of Zeolite and Selenium Application on Some Physiological Traits and Oil Yield of Medicinal Pumpkin (Cucurbita pepo L. under Drought Stress

    Directory of Open Access Journals (Sweden)

    Kourosh Eskandari Zanjani

    2012-07-01

    Full Text Available There is no doubt that certain plants offer valuable medicinal properties. But there are many challenges that local communities face in the harvesting and cultivation of these plants. Medicinal pumpkin (Cucurbita pepo L. var. Styriaca is an economically important medicinal plant cultivated for oil production. Water source limitation is one of the important problems in agriculture worldwide. Natural zeolits have some properties such as water absorption and emission and nitrate leaching inhibition, which is useful for soil amendment. This study was carried out to investigate the effects of zeolite and selenium application on some physiological traits and oil yield of medicinal pumpkin (Cucurbita pepo L. under drought stress. A field experiment was carried out in a factorial based on a randomized complete block design with three replications. The factors included: Irrigation (control, drought stress imposed during at flowering phase and drought stress imposed during fruit formation, Zeolite (non-application and application of 10 ton/ha and Selenium (nonapplication and 30 g/Lit from sodium selenate. All physiological parameters were affected by drought stress and zeolite application. Drought stress decreased total chlorophyll content, stomatal conductance, RWC, leaf soluble protein, oil content and oil yield and zeolite application in drought stress increased mentioned traits except oil content. Proline accumulation, WSD and CAT antioxidant enzyme activity were reduced in presence of zeolite in drought stress conditions than under control. Selenium spraying with zeolite application in drought conditions increased RWC, CAT antioxidant enzyme activity and oil yield. Drought stress at fruit formation phase reduced oil content and oil yield more severe than that on flowering stage. Finally, it seems that zeolite and selenium application in dry lands are exposed to drought stress be helpful for physiological traits and oil yield improvement and

  1. Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo

    Directory of Open Access Journals (Sweden)

    Vieira Iolanda Cruz

    2003-01-01

    Full Text Available Crude extracts of several vegetables such as peach (Prunus persica, yam (Alocasia macrorhiza, manioc (Manihot utilissima, artichoke (Cynara scolymus L, sweet potato (Ipomoea batatas (L. Lam., turnip (Brassica campestre ssp. rapifera, horseradish (Armoracia rusticana and zucchini (Cucurbita pepo were investigated as the source of peroxidase (POD: EC 1.11.1.7. Among those, zucchini (Cucurbita pepo crude extract was found to be the best one. This enzyme in the presence of hydrogen peroxide catalyses the oxidation of paracetamol to N-acetyl-p-benzoquinoneimine which the electrochemical reduction back to paracetamol was obtained at a peak potential of fraction three-quarters0.10V. A cyclic voltammetric study was performed by scanning the potential from + 0.5 to fraction three-quarters 0.5 V. The recovery of paracetamol from two samples ranged from 97.3 to 106% and a rectilinear calibration curve for paracetamol concentration from 1.2x10-4 to 2.5x10-3 mol L-1 (r=0.9965 were obtained. The detection limit was 6.9x10-5 mol L-1 and the relative standard deviation was less than 1.1% for a solution containing 2.5x10-3 mol L-1 paracetamol and 2.0x10-3 mol L-1 hydrogen peroxide (n=12. The results obtained for paracetamol in pharmaceutical products using the proposed biosensor and Pharmacopoeial procedures are in agreement at the 95% confidence level.

  2. Age-stage, two-sex life tables of Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae) with a discussion on the problem of applying female age-specific life tables to insect populations

    Institute of Scientific and Technical Information of China (English)

    Yu-Bing Huang; Hsin Chi

    2012-01-01

    Age-stage,two-sex life tables of the melon fly,Bactrocera cucurbitae ( Coquillett) (Diptera:Tephritidae),reared on cucumber (Cucumis sativus L.),sponge gourd (Luffa cylindrica Roem) and a carrot medium (mashed Daucus carota L.mixed with sucrose and yeast hydrolysate) were constructed under laboratory conditions at 25 ± 1 ℃,65% ± 0.5%relative humidity,and a photoperiod 12 ∶ 12h (L ∶ D).The intrinsic rates of increase of B.cucurbitae were 0.144 6,0.141 2 and 0.068 8 days on cucumber,sponge gourd,and carrot medium,respectively.The highest net reproduction rate was 172 offspring per fly reared on sponge gourd.The mean generation times of B.cucurbitae ranged from 34 days reared on cucumber to 56 days reared on carrot medium.The life history raw data was analyzed using the traditional female age-specific life table and compared to results obtained using the age-stage,two-sex life table.When the age-specific female life table is applied to an age-stage-structured two-sex population,survival and fecundity curves will be improperly manipulated due to an inability to include variation in preadult development time.We discussed different interpretations of the relationship between the net reproductive rate and the intrinsic rate of increase to clarify possible misunderstanding in the literature.

  3. USO DE CULTURAS INICIADORAS PARA A ELABORAÇÃO DE UM EMBUTIDO À BASE DE CARNE DE PATO (Cairina moschata USE OF STARTER CULTURES TO PRODUCE FERMENTED SAUSAGE FROM DUCK MEAT

    Directory of Open Access Journals (Sweden)

    Felipe Oliveira CARIONI

    2001-12-01

    Full Text Available No Brasil, tanto a criação quanto a comercialização da carne de pato (Cairina moschata ainda é incipiente, mas devido a sua rusticidade, fácil manejo, e carne apreciada no mercado, entende-se que a obtenção de produtos à base de carne de pato apresenta-se como uma alternativa rentável na indústria de alimentos. O uso de culturas iniciadoras na elaboração de produtos fermentados é uma prática comum na indústria de produtos cárneos estando diretamente relacionada às características de flavor, textura, cor e vida-de-prateleira do produto final. Avaliou-se o uso de culturas iniciadoras na elaboração de embutido fermentado à base de carne de pato obtida da desossa manual da coxa e sobrecoxa. Uma mistura de culturas iniciadoras de Lactobacillus plantarum BN e Kokuria varians CCT 4492 foi usada para inocular a massa cárnea. Os embutidos foram defumados em câmara para defumação a 23±1°C por aproximadamente 19 horas e curados por 25 dias. A contagem inicial de células viáveis na massa do embutido foi de 6,08Log10UFC/g e de 6,04Log10UFC/g para bactérias ácido-lácticas e Micrococcacea, respectivamente. Após o segundo dia do processo, bactérias ácido-lácticas apresentaram um crescimento de 0,79 ciclos logarítmicos e no décimo primeiro dia um aumento de 2,58 ciclos logarítmicos. O valor médio de acidez, em ácido láctico, no produto final foi de 0,39% e o valor de pH de 5,11. As análises físico-químicas apresentaram-se dentro dos padrões da legislação brasileira. O produto elaborado apresentou perfil sensorial dentro dos padrões aceitáveis de qualidade.In Brazil, duck raising (Cairina moschata and duck meat commercialization are still incipient activities, but due to this animal's rusticity, easiness of management and the marketability of its meat, they may be seen as profitable alternatives in the food industry. The use of starters in the manufacturing of fermented products is a common practice in the meat

  4. Thick root of cucumber: other susceptible plants and the effect of pH

    NARCIS (Netherlands)

    Gaag, van der D.J.; Paternotte, P.; Hamelink, R.

    2002-01-01

    Thick root is a relatively new disorder of cucumber grown in artificial substrates. Plants of cucumber, tomato, sweet pepper, lupin, anthurium, Cucurbita ficifolia, C. maxima and two lines from crosses between C. maxima and C. moschata were grown in thick root disease (TRD)-infested nutrient solutio

  5. Is Grafting Useful for Managing Root-Knot Nematodes in Watermelon?

    Science.gov (United States)

    Five Citrullus lanatus var. citroides germplasm lines, four Lagenaria siceraria cultivars, one Cucurbita moschata x C. maxima hybrid, and one commercial wild watermelon (Citrullus lanatus spp.) cultivar were evaluated as rootstocks for watermelon in a field infested with the southern root-knot nemat...

  6. Grafting for Management of Root-Knot Nematodes in Watermelon

    Science.gov (United States)

    Five wild watermelon (Citrullus lanatus var. citroides) germplasm lines, four bottlegourd (Lagenaria siceraria) cultivars, one squash (Cucurbita moschata x C. maxima) hybrid, and one commercial wild watermelon (C. lanatus spp.) cultivar were evaluated as rootstocks for watermelon in a field infested...

  7. Partially resistant Cucurbita pepo showed late onset of the Zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar

    Directory of Open Access Journals (Sweden)

    Slavomira eNovakova

    2015-04-01

    Full Text Available Zucchini yellow mosaic virus (ZYMV is an emerging viral pathogen in cucurbit-growing areas wordwide. Infection causes significant yield losses in several species of the family Cucurbitaceae. To identify proteins potentially involved with resistance towards infection by the severe ZYMV-H isolate, two Cucurbita pepo cultivars (Zelena susceptible and Jaguar partially resistant were analyzed using a two-dimensional gel electrophoresis-based proteomic approach. Initial symptoms on leaves (clearing veins developed 6-7 days post inoculation (dpi in the susceptible C. pepo cv. Zelena. In contrast, similar symptoms appeared on the leaves of partially resistant C. pepo cv. Jaguar only after 15 dpi. This finding was confirmed by immune-blot analysis which showed higher levels of viral proteins at 6 dpi in the susceptible cultivar. Leaf proteome analyses revealed 28 and 31 spots differentially abundant between cultivars at 6 and 15 dpi, respectively. The variance early in infection can be attributed to a rapid activation of proteins involved with redox homeostasis in the partially resistant cultivar. Changes in the proteome of the susceptible cultivar are related to the cytoskeleton and photosynthesis.

  8. Macrocospic and physiochemical characterization of a sugarless and gluten-free cake enriched with fibers made from pumpkin seed (Cucurbita maxima, L. flour and cornstarch

    Directory of Open Access Journals (Sweden)

    Cristiane Mesquita da Silva Gorgônio

    2011-03-01

    Full Text Available The Consumers' interest for products with caloric reduction has increased, and their development is a technological challenge. The consumption of cakes has grown in importance and the demand for dietary products has stimulated the use of sweeteners and the optimization of bakery products. The consumption of fibers is related to chronic diseases prevention. Pumpkin seeds (maximum Cucurbita, L., rich in fibers, can be used as a source of fiber in food products. A gluten-free diet is not easy to follow since gluten free products are not always available. The objective of this work was to perform a physicochemical characterization of cakes prepared with flours blends (FB based on Pumpkin Seed Flour (PSF. The cakes were elaborated with FB in the ratios of 30:70 (C30 and 40:60 (C40 of PSF and cornstarch (CS, respectively. The results showed gluten absence and near-neutral pH. The chemical analysis of C30 and B40 showed increase of ashes, lipids, proteins, and insoluble dietary fiber and a decrease in the content of carbohydrates and calories. The chemical composition of C40 presented the greatest content of lipids, proteins, and dietary fibers, the lowest content of calories, and the best physical parameters. Therefore, both products proved suitable for human consumption.

  9. Application of cavitation system to accelerate aqueous enzymatic extraction of seed oil from Cucurbita pepo L. and evaluation of hypoglycemic effect.

    Science.gov (United States)

    Li, Xiao-Juan; Li, Zhu-Gang; Wang, Xun; Han, Jun-Yan; Zhang, Bo; Fu, Yu-Jie; Zhao, Chun-Jian

    2016-12-01

    Cavitation-accelerated aqueous enzymatic extraction (CAEE) of seed oil from Cucurbita pepo was performed. An enzyme cocktail comprised of cellulose, pectinase and proteinase can work synergistically in releasing the oil. The CAEE extraction conditions were optimized by a Plackett-Burman design followed by a central composite methodology. A maximal extraction yield of 58.06% was achieved under optimal conditions of vacuum degree -0.07, enzyme amount 1.05% and extraction time 69min. As compared to soxhlet extraction (SE)-derived oil, CAEE-derived oil exhibited similar physical properties and better oxidation stability. In addition, chemical composition analyzing showed that the content of linoleic acid obtained by CAEE (47.67%) was higher than that of SE (44.51%). Moreover, the IC50 of oil obtained by CAEE and SE, as measured by α-amylase inhibition assay, were 40.68μg/mL and 45.46μg/mL. All results suggest that CAEE represents an excellent alternative protocol for production of oil from oil-bearing materials.

  10. Effects of head pruning and different nutritional systems (chemical, biological and integrated on seed yield and oil content in medicinal pumpkin (Cucurbita pepo L.

    Directory of Open Access Journals (Sweden)

    Zarei Dariush

    2016-01-01

    Full Text Available To evaluate the effect of head pruning and different nutritional systems (chemical, biological and integrated on yield and seed oil content in medicinal pumpkin (Cucurbita pepo L., an experiment was conducted in Kermanshah/Iran during the 2013 growing season. The experimental treatments consisted of two levels - no head pruning, control (Co and head pruning (C1 allocated to the main plots. Four levels of different fertilizing systems - control (without fertilizer (T0, chemical (T1, biological (a combination of nitrogen fixing bacteria, Azospirillum brasilense and Glomus mosseae (T2, and integrated fertilizing system (biological fertilizer + 50% chemical fertilizer (T3 were assigned to the sub-plots. The experimental treatments were arranged as a split plot based on a randomized complete block design with three replications. The results showed that the highest percentage of seed oil was obtained (37% in the integrated nutritional system along with the head pruning treatment. The highest grain yields of 53 and 50 g per square meter were obtained in integrated and chemical fertilizing systems, respectively while no pruning was applied. The highest fruit yields of 3,710 and 3,668 kg per hectare were produced by chemical and integrated fertilizing systems, respectively. The biological nutrition system required more time to demonstrate its positive effect on the growth and yield of medicinal pumpkin.

  11. Amino acid, mineral and fatty acid content of pumpkin seeds (Cucurbita spp) and Cyperus esculentus nuts in the Republic of Niger.

    Science.gov (United States)

    Glew, R H; Glew, R S; Chuang, L-T; Huang, Y-S; Millson, M; Constans, D; Vanderjagt, D J

    2006-06-01

    Dried seeds and nuts are widely consumed by indigenous populations of the western Sahel, especially those who inhabit rural areas. In light of the need for quantitative information regarding the content of particular nutrients in these plant foods, we collected dried pumpkin (Cucurbita spp) seeds and nuts of Cyperus esculentus in the Republic of Niger and analyzed them for their content of essential amino acids, minerals and trace elements, and fatty acids. On a dry weight basis, pumpkin seed contained 58.8% protein and 29.8% fat. However, the lysine score of the protein was only 65% relative to the FAO/WHO protein standard. The pumpkin seed contained useful amounts of linoleic (92 microg/g dry weight) and the following elements (on a microg per g dry weight basis): potassium (5,790), magnesium (5,690), manganese (49.3), zinc (113), selenium (1.29), copper (15.4), chromium (2.84), and molybdenum (0.81), but low amounts of calcium and iron. Except for potassium (5,573 microg/g dry weight) and chromium (2.88 microg/g dry weight), the C. esculentis nuts contained much less of these same nutrients compared to pumpkin seeds. In conclusion, pumpkin seeds represent a useful source of many nutrients essential to humans. The data in this report should of practical value to public health officials in rural areas of sub-Saharan Africa.

  12. Effect of oil extraction assisted by ultrasound on the physicochemical properties and fatty acid profile of pumpkin seed oil (Cucurbita pepo).

    Science.gov (United States)

    Hernández-Santos, Betsabé; Rodríguez-Miranda, Jesús; Herman-Lara, Erasmo; Torruco-Uco, Juan G; Carmona-García, Roselis; Juárez-Barrientos, José M; Chávez-Zamudio, Rubí; Martínez-Sánchez, Cecilia E

    2016-07-01

    The effects of amplitude and time of ultrasound-assisted extraction on the physicochemical properties and the fatty acid profile of pumpkin seed oil (Cucurbita pepo) were evaluated. Ultrasound time (5-30 min) and the response variables amplitude (25-100%), extraction yield, efficiency, oxidative stability in terms of the free fatty acids (FFA) of the plant design comprising two independent experiments variables, peroxide (PV), p-anisidine (AV), totox value (TV) and the fatty acid profile were evaluated. The results were analyzed by multiple linear regression. The time and amplitude showed significant differences (P<0.05) for all variables. The highest yield of extraction was achieved at 5 min and amplitude of 62.5% (62%). However, the optimal ultrasound-assisted extraction conditions were as follows: ultrasound time of 26.34 min and amplitude of 89.02%. All extracts showed low FFA (2.75-4.93% oleic acid), PV (1.67-4.68 meq/kg), AV (1.94-3.69) and TV (6.25-12.55) values. The main fatty acids in all the extracts were oleic and linoleic acid. Therefore, ultrasound-assisted oil extraction had increased performance and reduced extraction time without affecting the oil quality.

  13. Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCl4-induced liver injury in low-protein fed rats.

    Science.gov (United States)

    Nkosi, C Z; Opoku, A R; Terblanche, S E

    2005-04-01

    The effects of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of lactate dehydrogenase (LD), alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) against carbon tetrachloride (CCl4)-induced acute liver injury in low-protein fed rats were investigated. A group of male Sprague-Dawley rats maintained on a low-protein diet for 5 days were divided into three subgroups. Two subgroups were injected with carbon tetrachloride and the other group with an equivalent amount of olive oil. Two hours after CCl4 intoxication one of the two subgroups was administered with pumpkin seed protein isolate. All three subgroups of rats were maintained on the low-protein diet for the duration of the investigation. Groups of rats from the different subgroups were killed at 24, 48 and 72 h after their respective treatments. After 5 days on the low-protein diet the activity levels of all four enzymes were significantly higher than their counterparts on a normal balanced diet. CCl4 intoxication resulted in significant increases in the activity levels of all four enzymes investigated. The administration of pumpkin seed protein isolate after CCl4 intoxication resulted in significantly reduced activity levels of all four enzymes. It is concluded that pumpkin seed protein isolate administration was effective in alleviating the detrimental effects associated with protein malnutrition.

  14. Overexpression of the pumpkin (Cucurbita maxima) 16 kDa phloem protein CmPP16 increases tolerance to water deficit.

    Science.gov (United States)

    Ramírez-Ortega, Francisco Arturo; Herrera-Pola, Paul Starsky; Toscano-Morales, Roberto; Xoconostle-Cázares, Beatriz; Ruiz-Medrano, Roberto

    2014-01-01

    The phloem plays an important role in the delivery of nutrients and signals between photosynthetic to heterotrophic tissues. Proteins and RNAs in the phloem translocation stream may have an important role in maintaining the integrity of the sieve tube system, as well as in long-distance signaling. CmPP16 is a pumpkin phloem protein, which has been shown to bind RNA in a non-sequence specific manner, and move it cell-to-cell and conceivably, long-distance. The protein and RNA are found in both companion cell (CC) and sieve elements (SE). However, a more precise function for this protein is not known. In this work we report the overexpression of CmPP16 fused to GFP via transformation of pumpkin (Cucurbita maxima cv. Big Max) plants in the cotyledonary stage by direct inoculation of Agrobacterium tumefaciens and Agrobacterium rhizogenes. Plants overexpressing CmPP16 did not show an obvious phenotype. However, these plants displayed higher photosynthetic capacity during drought than wild-type (WT) pumpkin or transformed with another construct. These results suggest that CmPP16 may be involved in the response to stress through long-distance signaling.

  15. Natural abundance 15N NMR assignments delineate structural differences between intact and reactive-site hydrolyzed Cucurbita maxima trypsin inhibitor III.

    Science.gov (United States)

    Krishnamoorthi, R; Nemmers, S; Tobias, B

    1992-06-15

    15N NMR assignments were made to the backbone amide nitrogen atoms at natural isotopic abundance of intact and reactive-site (Arg5-Ile6) hydrolyzed Cucurbita maxima trypsin inhibitor III (CMTI-III and CMTI-III*, respectively) by means of 2D proton-detected heteronuclear single bond chemical shift correlation (HSBC) spectroscopy, utilizing the previously made sequence-specific 1H NMR assignments (Krishnamoorthi et al. (1992) Biochemistry 31, 898-904). Comparison of the 15N chemical shifts of the two forms of the inhibitor molecule revealed significant changes not only for residues located near the reactive-site region, but also for those distantly located. Residues Cys3, Arg5, Leu7, Met8, Cys10, Cys16, Glu19, His25, Tyr27, Cys28 and Gly29 showed significant chemical shift changes ranging from 0.3 to 6.1 ppm, thus indicating structural perturbations that were transmitted throughout the molecule. These findings confirm the earlier conclusions based on 1H NMR investigations.

  16. Methyl jasmonate is a more effective senescence-promoting factor in Cucurbita pepo (zucchini) cotyledons when compared with darkness at the early stage of senescence.

    Science.gov (United States)

    Ananieva, Kalina; Ananiev, Evgueni D; Mishev, Kiril; Georgieva, Katya; Malbeck, Jiri; Kamínek, Miroslav; Van Staden, Johannes

    2007-09-01

    The effects of short-term darkening and methyl jasmonate (MeJA) on cotyledon senescence were studied 24h after transfer of intact 7-day-old Cucurbita pepo (zucchini) seedlings to darkness or spraying with 100 microM MeJA. The jasmonate inhibitory effect on chlorophyll content and chloroplast transcriptional activity was stronger compared with darkness. Further, MeJA reduced the photosynthetic rate whereas darkness did not affect photosynthesis. Neither stress factor affected the photochemical quantum efficiency of photosystem II (PSII) estimated by the variable fluorescence (F(v))/maximal fluorescence (F(m)) ratio, suggesting the existence of mechanisms protecting the functional activity of PSII at earlier stages of senescence, thus making this parameter more stable compared to others used to quantify senescence. Both stress factors caused a decrease in the content of physiologically active cytokinins, especially trans-zeatin (Z), with the jasmonate effect being much more pronounced when compared to darkness. Our results indicate that MeJA is a more potent inducer of senescence in zucchini cotyledons, at least within the relatively short period of the 24h treatment. This is likely due to its stronger down-regulatory effect on the levels of physiologically active cytokinins.

  17. Partially resistant Cucurbita pepo showed late onset of the Zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar.

    Science.gov (United States)

    Nováková, Slavomíra; Flores-Ramírez, Gabriela; Glasa, Miroslav; Danchenko, Maksym; Fiala, Roderik; Skultety, Ludovit

    2015-01-01

    Zucchini yellow mosaic virus (ZYMV) is an emerging viral pathogen in cucurbit-growing areas wordwide. Infection causes significant yield losses in several species of the family Cucurbitaceae. To identify proteins potentially involved with resistance toward infection by the severe ZYMV-H isolate, two Cucurbita pepo cultivars (Zelena susceptible and Jaguar partially resistant) were analyzed using a two-dimensional gel electrophoresis-based proteomic approach. Initial symptoms on leaves (clearing veins) developed 6-7 days post-inoculation (dpi) in the susceptible C. pepo cv. Zelena. In contrast, similar symptoms appeared on the leaves of partially resistant C. pepo cv. Jaguar only after 15 dpi. This finding was confirmed by immune-blot analysis which showed higher levels of viral proteins at 6 dpi in the susceptible cultivar. Leaf proteome analyses revealed 28 and 31 spots differentially abundant between cultivars at 6 and 15 dpi, respectively. The variance early in infection can be attributed to a rapid activation of proteins involved with redox homeostasis in the partially resistant cultivar. Changes in the proteome of the susceptible cultivar are related to the cytoskeleton and photosynthesis.

  18. L-ascorbic acid determination in pharmaceutical formulations using a biosensor based on carbon paste modified with crude extract of zucchini (Cucurbita pepo

    Directory of Open Access Journals (Sweden)

    Fatibello-Filho Orlando

    2000-01-01

    Full Text Available A biosensor based on carbon paste modified with crude extract of zucchini (Cucurbita pepo as a source of peroxidase is proposed for determining L-ascorbic acid in pharmaceutical formulations. This enzyme in the presence of hydrogen peroxide catalyses the oxidation of hydroquinone to p-quinone whose electrochemical reduction back to hydroquinone was obtained at peak potential of -0.14V. Thus, when L-ascorbic acid is added to the solution, this acid can reduce chemically p-quinone to hydroquinone and/or reduce hydrogen peroxide, decreasing the peak current obtained proportionally to the increase of its concentration. The recovery of L-ascorbic acid from five samples ranged from 98.1 to 102.1% and a rectilinear calibration curve for L-ascorbic acid concentration from 2.0x10-4 to 5.5x10-3 mol L-1 (r=0.9992 was obtained. The detection limit was 2.2x10-5 mol L-1 and relative standard deviation was < 1.3% for a solution containing 4.0x10-3 mol L-1 L-ascorbic acid, 7.0x10-3 mol L-1 hydroquinone and 2.0x10-4 mol L-1 hydrogen peroxide. The results obtained for L-ascorbic acid in pharmaceutical formulations using the proposed biosensor and those obtained using the Pharmacopeia method are in agreement at the 95 % confidence level.

  19. Immunologically related lectins from stems and roots of developing seedlings of Cucurbita ficifolia: purification and some properties of root and stem lectins

    Directory of Open Access Journals (Sweden)

    Irena Lorenc-Kubis

    2014-02-01

    Full Text Available Hemagglutinating activity has been found in acetate extracts from roots and stems of squash seedlings (Cucurbita ficifolia. The hemaglutinating activity changes during seeds germination and seedling development. Dot blot and Western blot techniques have shown that proteins from these vegetative tissues cross-reacted with antibodies raised against endogenous cotyledons lectin CLBa and Con A.Lectins were isolated from stems and roots of 6-day old seedlings by precipitation with ethanol, affinity chromatography on Con A-Sepharose, gel filtration on Bio-gel P100 and separated by electrophoresis on polyacrylamide gel. Three purified lectins (RLA1, RLA2, RLA3 were obtained from roots and four from stems (SLA1, SLA2, SLA3, SLA4. The purified lectins from roots and stems agglutinated all human red blood cells, but sheep erythrocytes were most sensitive to agglutination. The hemagglutination of the root lectins RLA2 and RLA3 was inhibited by a very low concentration of arabinose, while RLA1, of xylose and Ga1NAc. Arabinose and Xylose were also found to be the most effective inhibitors of all stem lectins.

  20. INHIBICIÓN DEL CRECIMIENTO DE Giardia lamblia POR ACCIÓN DEL EXTRACTO ACUOSO Y METANÓLICO DE SEMILLAS DE Cucurbita pepo

    Directory of Open Access Journals (Sweden)

    María Porfiria Barrón González

    2010-02-01

    Full Text Available Giardia lamblia es el protozoario parásito causante de la giardiasis, la cual se caracteriza por molestia abdominal, pérdida de peso y desnutrición. La droga de elección para su tratamiento es el metronidazol sin embargo, presenta diversos efectos secundarios adversos en el paciente. Por otra parte, en la herbolaria se conoce a la semilla de Cucurbita pepo por sus propiedades desparasitante, principalmente sobre Entamoeba histolytica y Taenia solium. Por lo anterior se planteó la hipótesis de que las semillas de C. pepo contienen principios activos capaces de inhibir el crecimiento de G. lamblia bajo condiciones axénicas in vitro. El objetivo de esta investigación fue evaluar la actividad biológica del extracto acuoso y metanólico de las semillas de C. pepo sobre el crecimiento de G. lamblia. Tanto el extracto metanólico como el acuoso se identificaron siete grupos funcionales y la actividad antioxidante no fue significativa

  1. Zinc finger protein genes from Cucurbita pepo are promising tools for conferring non-Cucurbitaceae plants with ability to accumulate persistent organic pollutants.

    Science.gov (United States)

    Inui, Hideyuki; Hirota, Matashi; Goto, Junya; Yoshihara, Ryouhei; Kodama, Noriko; Matsui, Tomomi; Yamazaki, Kiyoshi; Eun, Heesoo

    2015-03-01

    Some cultivars of cucumbers, melons, pumpkins, and zucchini, which are members of the Cucurbitaceae family, are uniquely subject to contamination by hydrophobic pollutants such as the organohalogen insecticides DDT. However, the molecular mechanisms for the accumulation of these pollutants in cucurbits have not been determined. Here, cDNA subtraction analysis of Cucurbita pepo cultivars that are low and high accumulators of hydrophobic contaminants revealed that a gene for zinc finger proteins (ZFPs) are preferentially expressed in high accumulators. The cloned CpZFP genes were classified into 2 types: (1) the PBG type, which were expressed in C. pepo cultivars Patty Green, Black Beauty, and Gold Rush, and (2) the BG type, which were expressed in Black Beauty and Gold Rush. Expression of these CpZFP genes in transgenic tobacco plants carrying an aryl hydrocarbon receptor-based inducible gene expression system significantly induced β-glucuronidase activity when the plants were treated with a polychlorinated biphenyl (PCB) compound, indicating that highly hydrophobic PCBs accumulated in the plants. In transgenic tobacco plants carrying CpZFPs, accumulation of dioxins and dioxin-like compounds increased in their aerial parts when they were cultivated in the dioxin-contaminated soil. In summary, we propose that addition of CpZFP genes is a promising tool for conferring noncucurbits with the ability to accumulate hydrophobic contaminants.

  2. Effects of different levels of Pumpkin (Cucurbita Pepo residue silage replacement with forage part of ration on male buffalo calves fattening performance

    Directory of Open Access Journals (Sweden)

    A. Hashemi

    2010-02-01

    Full Text Available An experiment was conducted to study the effects of replacing forage part of ration with different levels of Pumpkin (Cucurbita Pepo residual silage (PRS on fattening performance of male buffalo calves. Twenty Four calves with 203 + 27.9 Kg of BW fed experimental diets in a completely randomized design with 4 groups and 6 replicates. The four groups (1, 2, 3 and 4 included 0 (control, 20, 40 and 60 percent replacement of forage part of ration with PRS, respectively in basal diet. The results showed that dry matter intake (DMI was not significantly different between the groups. The highest and the lowest DMI were shown at the groups 1 and 4, respectively. Daily weight gain (DWG was not significantly different between the groups. The highest and the lowest DWG were shown at the groups 3 and 2, respectively. Feed conversion ratio (FCR was not significantly different between the groups. But the best FCR was obtained at group 1. It may conclude that forage part of ration can be substituted with PRS at 60 % level with no negative effects on male buffalo calves fattening performance.

  3. Insecticidal activity of the leaf essential oil of Peperomia borbonensis Miq. (Piperaceae) and its major components against the melon fly Bactrocera cucurbitae (Diptera: Tephritidae).

    Science.gov (United States)

    Dorla, Emmanuelle; Bialecki, Anne; Deuscher, Zoé; Allibert, Agathe; Grondin, Isabelle; Deguine, Jean-Philippe; Laurent, Philippe

    2017-03-08

    The essential oil from leaves of Peperomia borbonensis from Réunion Island was obtained by hydrodistillation and characterized using GC-FID, GC-MS and NMR. The main components were myristicin (39.5%) and elemicin (26.6%). The essential oil (EO) of Peperomia borbonensis and its major compounds (myristicin and elemicin), pure or in a mixture, were evaluated for their insecticidal activity against Bactrocera cucurbitae (Diptera: Tephritidae) using a filter paper impregnated bioassay. The concentrations necessary to kill 50% (LC50 ) and 90% (LC90 ) of the flies in three hours were determined. The LC50 was 0.23 ± 0.009 mg/cm² and the LC90 was 0.34 ± 0.015 mg/cm² for the EO. The median lethal time (LT50 ) was determined to compare the toxicity of EO and the major constituents. The EO was the most potent insecticide (LT50 = 98 ± 2 min), followed by the mixture of myristicin and elemicin (1.4:1) (LT50 = 127± 2 min) indicating that the efficiency of the EO is potentiated by minor compounds and emphasizing one of the major assets of EOs against pure molecules. This article is protected by copyright. All rights reserved.

  4. Effects of temperature and light on take-off activity of Bactrocera cucurbitae%温度、光照强度对瓜实蝇成虫飞行行为的影响

    Institute of Scientific and Technical Information of China (English)

    崔志富; 曹凤勤; 程立生; 符悦冠; 万方浩; 张桂芬

    2016-01-01

    [Background] Bactrocera cucurbitae is the important pest of Amphisarca in Sourthern China. The objective of this study was to examine the fly movement of B. cucurbitae to between control this fly. [Method] Based on the known biological characteris-tics, artificial climate chamber and some other equipment were used to study the effects of temperature and light changes on fly be-havior activity of B. cucurbitae. [Result] The results showed that the fly activities increased with temperature starting at 15℃ with an optimum temperature between 20 and 30 ℃. Flying activities were obviously the greatest at temperatures above 35 ℃ and the least when temperatures dropped under 10℃. The activity percent of B. cucurbitae is more than 50% at 25~40℃, but it is 0% at 10、15 and 45 ℃ respectively. Light intensity also affected fly behavior with fly activities increasing over 2400 lx and reaching a peak at 9600 lx. Extreme light intensity (12000 lx) inhibited flight behavior. The number of take-off activity of B. cucurbitae increased with the increase of light intensity.[Conclusion and significance] Extremes in temperature and light intensity may limit flight activity of B. cucurbitae. Moderate high temperature can encourage fly behavior.%【背景】瓜实蝇是我国南方瓜果的主要害虫。本试验分析了影响瓜实蝇成虫飞行行为及活跃度的原因,以便能更有效地控制瓜实蝇危害。【方法】在已知瓜实蝇的生物学特性及部分活动规律的基础上利用智能人工气候箱等设施,分别测定温度、光照对瓜实蝇的飞行行为的影响。【结果】瓜实蝇成虫在15℃下开始飞行,飞行活动的群体数量随着温度的升高而增加,最适飞行活动温度范围为20~30℃,低温10℃明显对其飞行行为有抑制作用,高温(35℃以上)明显对其有刺激作用,促进其飞行行为的发生。瓜实蝇成虫的活跃度在25~40℃下较高,均大于50%,在10、15和45℃下则为0

  5. Specific Water Quality Sites for Duchesne County, Utah

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This map shows specific water-quality items and hydrologic data site information which come from QWDATA (Water Quality) and GWSI (Ground Water Information System)....

  6. Insect Distribution and Habitat, Grasshop, Published in 2004, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Insect Distribution and Habitat dataset, was produced all or in part from Other information as of 2004. It is described as 'Grasshop'. Data by this publisher...

  7. Election Districts and Precincts, Fruitland, Published in 2001, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Election Districts and Precincts dataset, was produced all or in part from Other information as of 2001. It is described as 'Fruitland'. Data by this publisher...

  8. Election Districts and Precincts, Neola, Published in 2001, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Election Districts and Precincts dataset, was produced all or in part from Other information as of 2001. It is described as 'Neola'. Data by this publisher are...

  9. Cellular Phone Towers, Towers, Published in 2008, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Cellular Phone Towers dataset, was produced all or in part from Other information as of 2008. It is described as 'Towers'. Data by this publisher are often...

  10. Trails, RS2477, picpts, Published in 2006, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Trails, RS2477 dataset, was produced all or in part from Other information as of 2006. It is described as 'picpts'. Data by this publisher are often provided in...

  11. Flood Control Structures, POD 77, Published in 2001, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Flood Control Structures dataset, was produced all or in part from Other information as of 2001. It is described as 'POD 77'. Data by this publisher are often...

  12. Zoning Districts, ag-res, Published in 2008, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Zoning Districts dataset, was produced all or in part from Other information as of 2008. It is described as 'ag-res'. Data by this publisher are often provided...

  13. Canals, Bypass Canal, Published in 2002, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Canals dataset, was produced all or in part from Other information as of 2002. It is described as 'Bypass Canal'. Data by this publisher are often provided in...

  14. The Evaluation of Dipeptidyl Peptidase (DPP)-IV, α-Glucosidase and Angiotensin Converting Enzyme (ACE) Inhibitory Activities of Whey Proteins Hydrolyzed with Serine Protease Isolated from Asian Pumpkin (Cucurbita ficifolia)

    OpenAIRE

    Konrad, Babij; Anna, Dąbrowska; Marek, Szołtysik; Marta, Pokora; Aleksandra, Zambrowicz; Józefa, Chrzanowska

    2014-01-01

    In the present study, whey protein concentrate (WPC-80) and β-lactoglobulin were hydrolyzed with a noncommercial serine protease isolated from Asian pumpkin (Cucurbita ficifolia). Hydrolysates were further fractionated by ultrafiltration using membranes with cut-offs equal 3 and 10 kDa. Peptide fractions of molecular weight lower than 3 and 3–10 kDa were further subjected to the RP-HPLC. Separated preparations were investigated for their potential as the natural inhibitors of dipeptidyl pepti...

  15. 瓜实蝇DNA甲基化的MSAP体系建立与优化%Establishment and optimization of MSAP Analysis System for DNA Methylation in Bactrocera cucurbitae

    Institute of Scientific and Technical Information of China (English)

    龚治; 周世豪; 马华博; 张亚楠; 符悦冠

    2016-01-01

    The melon fly [Bactrocera cucurbitae (Coquillett)] is one of the most important vegetables insect pest in China. However, the DNA methylation in melon fly has not been reported yet. Methylation sensitive amplified polymorphism (MSAP) is an important research tecnnique to analysis the DNA methylation. The MSAP can be established through the enzyme digestion reaction, ligation, PCR amplification system, and primer screening, such as 10 U restriction endonuclease is put in the 20 μL enzyme digestion system, and then react with 600 ng of genome DNA at 37℃ overnight; 1U of T4 ligase is put in the 20 μL ligation system, 50 pmol of HpaⅡ-MspⅠ-adapter and 5 pmol of EcoR I-adapter which react at 16℃ for 12 hours. Diluent ligation products were used in PCR to pre-amplification and selected amplification, the result is tested by silver staining and 6% denaturing polyacrylamide gel electrophoresis. Six pairs of primers which suited for DNA methylation sensitive amplified polymorphism were selected out by this system. The MSAP system provides the technical support for the study of the epigenetics research of melon fly.%瓜实蝇[Bactrocera cucurbitae (Coquillett)]是中国重要的蔬菜害虫,但其DNA甲基化研究尚未见报道。甲基化敏感扩增多态性是研究DNA甲基化的重要技术之一。通过对酶切反应、连接、 PCR扩增和引物筛选等条件优化,建立瓜实蝇MSAP反应体系,即:①20μL酶切体系中加入10 U的限制性内切酶与600 ng基因组DNA,于37℃酶切反应过夜;②20μL连接体系中加入T4连接酶1 U, HpaⅡ-MspⅠ-adapter接头50 pmol, EcoR I-adapter接头5 pmol,并于16℃反应12 h;③连接产物稀释后进行PCR预扩增和选择性扩增,再经6%变性聚丙烯酰胺凝胶电泳和银染检测结果。通过该体系筛选出适用于瓜实蝇基因组DNA甲基化多态性研究的6对引物;瓜实蝇MSAP体系为瓜实蝇的表观遗传学研究提供了技术支持。

  16. Use of video surveillance to measure the influences of habitat management and landscape composition on pollinator visitation and pollen deposition in pumpkin (Cucurbita pepo agroecosystems

    Directory of Open Access Journals (Sweden)

    Benjamin W. Phillips

    2015-11-01

    Full Text Available Pumpkin (Cucurbita pepo production relies on insect-mediated pollination, which is provided by managed and wild pollinators. The goals of this study were to measure the visitation frequency, longevity and temporal activity patterns of pumpkin pollinators and to determine if local habitat management and landscape composition affected this pollination service. We used video surveillance to monitor bee acitivty within male and female pumpkin flowers in 2011 and 2012 across a pollination window of 0600–1200 h. We also quantified the amount of pollen deposited in female flowers across this time period. In 2011, A. mellifera made significantly more floral visits than other bees, and in 2012 Bombus spp. was the dominant pumpkin pollinator. We found variation in visitation among male and female pumpkin flowers, with A. mellifera visiting female flowers more often and spending longer per visit within them than male flowers in both 2011 and 2012. The squash bee P. pruinosa visited male flowers more frequently in 2012, but individuals spent equal time in both flower sexes. We did not find variation in the timing of flower visitation among species across the observed pollination window. In both 2011 and 2012 we found that the majority of pollen deposition occurred within the first two hours (0600–0800 h of observation; there was no difference between the pollen deposited during this two-hour period and full pollination window (0600–1200 h. Local additions of sweet alyssum floral strips or a field buffer strip of native wildflowers did not have an effect on the foraging activity of bees or pollen deposition. However, semi-natural and urban habitats in the surrounding landscape were positively correlated with the frequency of flower visitation by wild pollinators and the amount of pollen deposited within female flowers.

  17. Use of video surveillance to measure the influences of habitat management and landscape composition on pollinator visitation and pollen deposition in pumpkin (Cucurbita pepo) agroecosystems.

    Science.gov (United States)

    Phillips, Benjamin W; Gardiner, Mary M

    2015-01-01

    Pumpkin (Cucurbita pepo) production relies on insect-mediated pollination, which is provided by managed and wild pollinators. The goals of this study were to measure the visitation frequency, longevity and temporal activity patterns of pumpkin pollinators and to determine if local habitat management and landscape composition affected this pollination service. We used video surveillance to monitor bee acitivty within male and female pumpkin flowers in 2011 and 2012 across a pollination window of 0600-1200 h. We also quantified the amount of pollen deposited in female flowers across this time period. In 2011, A. mellifera made significantly more floral visits than other bees, and in 2012 Bombus spp. was the dominant pumpkin pollinator. We found variation in visitation among male and female pumpkin flowers, with A. mellifera visiting female flowers more often and spending longer per visit within them than male flowers in both 2011 and 2012. The squash bee P. pruinosa visited male flowers more frequently in 2012, but individuals spent equal time in both flower sexes. We did not find variation in the timing of flower visitation among species across the observed pollination window. In both 2011 and 2012 we found that the majority of pollen deposition occurred within the first two hours (0600-0800 h) of observation; there was no difference between the pollen deposited during this two-hour period and full pollination window (0600-1200 h). Local additions of sweet alyssum floral strips or a field buffer strip of native wildflowers did not have an effect on the foraging activity of bees or pollen deposition. However, semi-natural and urban habitats in the surrounding landscape were positively correlated with the frequency of flower visitation by wild pollinators and the amount of pollen deposited within female flowers.

  18. Antioxidant and anti-inflammatory effects of a hypoglycemic fraction from Cucurbita ficifolia Bouché in streptozotocin-induced diabetes mice.

    Science.gov (United States)

    Roman-Ramos, R; Almanza-Perez, J C; Fortis-Barrera, A; Angeles-Mejia, S; Banderas-Dorantes, T R; Zamilpa-Alvarez, A; Diaz-Flores, M; Jasso, I; Blancas-Flores, G; Gomez, J; Alarcon-Aguilar, F J

    2012-01-01

    Type 2 diabetes is characterized by oxidative stress and a chronic low-grade inflammatory state, which also play roles in the pathogenesis of this disease and the accompanying vascular complications by increasing the production of free radicals and pro-inflammatory cytokines. Cucurbita ficifolia Bouché (C. ficifolia) is an edible Mexican plant whose hypoglycemic activity has been demonstrated in several experimental and clinical conditions. Recently, D-chiro-inositol has been proposed as the compound responsible for the hypoglycemic effects; however, the antioxidant and anti-inflammatory potential of this plant has not yet been explored. The aim of this research is to study the influence of a hypoglycemic, D-chiro-inositol-containing fraction from the C. ficifolia fruit (AP-Fraction) on biomarkers of oxidative stress, as well as on the inflammatory cytokines in streptozotocin-induced diabetes. The AP-Fraction obtained from the mature fruit of C. ficifolia contained 3.31 mg of D-chiro-inositol/g of AP-Fraction. The AP-Fraction was administrated daily by gavage to normal mice for 15 days as a preventive treatment. Then these animals were given streptozotocin, and the treatments were continued for an additional 33 days. Pioglitazone was used as a hypoglycemic drug for comparison. Administration of the AP-Fraction significantly increased glutathione (GSH) and decreased malondialdehyde (MDA) in the liver without significantly affecting the levels in other tissues. The AP-Fraction reduced TNF-α and increased IL-6 and IFN-γ in serum. Interestingly, the AP-Fraction also increased IL-10, an anti-inflammatory cytokine. These results suggest that C. ficifolia might be used as an alternative medication for the control of diabetes mellitus and that it has antioxidant and anti-inflammatory properties in addition to its hypoglycemic activity.

  19. Direct and individual analysis of stress-related phytohormone dispersion in the vascular system of Cucurbita maxima after flagellin 22 treatment.

    Science.gov (United States)

    Furch, Alexandra C U; Zimmermann, Matthias R; Kogel, Karl-Heinz; Reichelt, Michael; Mithöfer, Axel

    2014-03-01

    • The stress-related phytohormones, salicylic acid (SA) and abscisic acid (ABA), and the three jasmonates, jasmonic acid (JA), cis-12-oxo-phytodienoic acid (cis-OPDA), and (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile), were investigated in phloem and xylem exudates of Cucurbita maxima. • Phloem and xylem exudates were separately collected and analysed via liquid chromatography-mass spectrometry. • We show direct evidence for all three jasmonates, ABA, and SA in both phloem and xylem exudates of C. maxima. JA and JA-Ile concentrations are higher in xylem (JA: c(xylem) ≈ 199.5 nM, c(phloem) ≈ 43.9 nM; JA-Ile: c(xylem) ≈ 7.9 nM, c(phloem) ≈ 1.6 nM), whereas ABA and SA concentrations are higher in phloem exudates (ABA: c(xylem) ≈ 37.1 nM, c(phloem) ≈ 142.6 nM; SA: c(xylem) ≈ 61.6 nM, c(phloem) ≈ 1319 nM). During bacteria-derived flagellin 22 (flg22)-triggered remote root-to-shoot signalling, phytohormone concentration changed rapidly both in phloem and xylem. • The unequal distribution of phytohormones suggests that phloem and xylem have distinct roles in defence responses. Our data shed light on systemic phytohormone signalling and help explain how plants cope with environmental challenges by lateral exchange between phloem and xylem. Our analysis is a starting point for further investigations of how phytohormones contribute to phloem- and xylem-based defence signalling. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

  20. Study on characteristics of polyphenol oxidase of Cucurbita pepo L.%西葫芦多酚氧化酶酶学特性研究

    Institute of Scientific and Technical Information of China (English)

    王国英; 董海洲; 王兆升; 刘传富

    2012-01-01

    The characteristics of polyphenol oxidase (PPO)of Cucurbita pepo L.and effect of various inhibitors to PPO were studied by spectrophotometer.The results showed that the optimum pH and temperature were 6.6 and 35℃. However,the PPQ was inactivated in 90℃ water for 5min.The kinetics of enzyme-catalyzed reaction of PPO was in accord with Michaelis-Menten equation,the Km, Vmax of kinetic equation were 0.0417mol/L,208.33U using catechol as substrate,respectively.Four kinds of inhibitors had different effects on PPO, and the order of PPO as NaHSO3〉 L-Cysteine 〉 ascorbic acid 〉 citric acid.%以邻苯二酚为底物,采用分光光度法对西葫芦多酚氧化酶(PPO)的酶学特性及不同抑制剂对多酚氧化酶活性的影响进行研究。结果表明,西葫芦多酚氧化酶最适pH为6.6,最适温度为35℃,90℃处理5rain可使该酶失活。多酚氧化酶催化的酶促褐变反应动力学符合米氏方程,动力学参数为Km=0.0417mol/L,Vmax=208.33U。四种抑制剂对PPO的抑制效果由强到弱依次为:亚硫酸氢钠〉L-半胱氨酸〉抗坏血酸〉柠檬酸。

  1. Cytotoxicity of hydro-alcoholic extracts of Cucurbita pepo and Solanum nigrum on HepG2 and CT26 cancer cell lines

    Directory of Open Access Journals (Sweden)

    M Shokrzadeh

    2010-01-01

    Full Text Available Plants are used worldwide for the treatment of diseases, and novel drugs continue to be developed through research from plants. There are more than 20,000 species of plants used in traditional medicines, and these are all potential reservoirs for new drugs. Cucurbita pepo has been used in traditional folk medicine to treat cold and alleviate ache. Previous pharmacological tests have shown that it possesses antiviral, anti-inflammatory, and analgesic effects. Also, Solanum nigrum has been used as a diuretic and an antipyretic agent and it has also been used to cure inflammation, edema, mastitis and hepatic cancer. In this investigation, cytotoxicity of specific concentrations of hydro-alcoholic extracts of C. pepo and S. nigrum was studied on normal [Chinese hamster ovarian cells (CHO and rat fibroblast] and cancer (HepG2 and CT26 cell lines. The cytotoxic effects and IC 50 of the extracts on the selected cell lines were studied followed by colonogenic assay method. The results showed that IC 50 of S. nigrum extract was significantly lower than that of the C. pepo extract on all four cell lines (P < 0.05. On the other hand, IC 50 of S. nigrum extract was significantly higher than the extract of Taxus baccata and Cisplatin, herbal and chemical control positive anticancer compounds, respectively, on all four cell lines (P < 0.05. As a result, it is concluded that the extract of S. nigrum has almost similar cytotoxicity to the extract of T. baccata on cancer cells.

  2. Cloning and characterisation of a putative pollen-specific polygalacturonase gene (CpPG1) differentially regulated during pollen development in zucchini (Cucurbita pepo L.).

    Science.gov (United States)

    Carvajal, F; Garrido, D; Jamilena, M; Rosales, R

    2014-03-01

    Studies in zucchini (Cucurbita pepo L. spp. pepo) pollen have been limited to the viability and morphology of the mature pollen grain. The enzyme polygalacturonase (PG) is involved in pollen development and pollination in many species. In this work, we study anther and pollen development of C. pepo and present the cloning and characterisation of a putative PG CpPG1 (Accession no. HQ232488) from pollen cDNA in C. pepo. The predicted protein for CpPG1 has 416 amino acids, with a high homology to other pollen PGs, such as P22 from Oenothera organensis (76%) and PGA3 from Arabidopsis thaliana (73%). CpPG1 belongs to clade C, which comprises PGs expressed in pollen, and presents a 34 amino acid signal peptide for secretion towards the cell wall. DNA-blot analysis revealed that there are at least another two genes that code for PGs in C. pepo. The spatial and temporal accumulation of CpPG1 was studied by semi-quantitative- and qRT-PCR. In addition, mRNA was detected only in anthers, pollen and the rudimentary anthers of bisexual flowers (only present in some zucchini cultivars under certain environmental conditions that trigger anther development in the third whorl of female flowers). However, no expression was detected in cotyledons, stem or fruit. Furthermore, CpPG1 mRNA was accumulated throughout anther development, with the highest expression found in mature pollen. Similarly, exo-PG activity increased from immature anther stages to mature anthers and mature pollen. Overall, these data support the pollen specificity of this gene and suggest an involvement of CpPG1 in pollen development in C. pepo.

  3. EST-SSR Markers Development from Cucurbita and Their Use in Purity Testing of F1 Hybrid Seed%南瓜属EST-SSR标记的开发及在杂种纯度鉴定中的应用

    Institute of Scientific and Technical Information of China (English)

    张国裕; 张帆; 姜立纲; 翟伟卜; 李海真

    2011-01-01

    Expressed Sequence Tags(ESTs) are a source of simple sequence repeats (SSRs) that can be used to develop molecular markers for genetic studies. The object of the present study is to develop EST-SSR markers from Cucurbita and uses these markers in quickly purity testing of F1 hybrid seed. The availability of 1457 ESTs for cucurbita,which documented in GenBank, provided a unique opportunity to develop EST-SSR markers, and 215 SSRs were identified among these non-redundant EST sequences. These SSRs contained 2 - 6 bp nucleotide motifs including 111 different motif types. The trinucleotide repeat is the dominant type,accounting for 40% with 86 motifs, and the frequency of occurrence of dinucleotide repeat is 28. 84% with 62 motifs. Among these motifs,CT motif (21 ,9. 77% ) was the most common type,and then were TC and AAG motifs,accounting for 6. 05% and 5. 58% , respectively. A total of 215 primer pairs were designed and 43 of them were synthesized and used to determine their usability by amplification in 4 Cucurbita inbred lines. The results show that 28 of them could successfully amplify, accounting for 65. 12% of testing primer pairs. Then these primer pairs were used to test the F, hybrid seed purity in Cucurbita. Seed genetic purity from 13 combinations ranged from 79. 2% to 100. 0% .which were in high accordance with those from field grow-out trials. Taken together, this study reported an effective and feasible approach to develop SSR markers for cucurbita,and demonstrated their usefulness in purity testing of hybrid squash seed.%为利用SSR标记进行快速、准确的南瓜杂交种纯度分析,进行了南瓜EST-SSR标记的开发.从NCBI数据库下载1 457条南瓜属作物EST序列,去除冗余序列后进行SSR位点搜索,共得到215条含有SSR位点的EST序列.这些SSR位点包含111种重复基元,其中二核苷酸(62个,占28.84%)和三核苷酸(86个,占40.0%)重复类型占主导地位;重复基元中出现最多的是CT(21个,占9.77

  4. Evaluation of microbial products for the control of zucchini foot and root rot caused by Fusarium solani f. sp. cucurbitae race 1

    Directory of Open Access Journals (Sweden)

    Roberta ROBERTI

    2012-09-01

    Full Text Available Microbial products containing bacteria (Cedomon [Pseudomonas chlororaphis MA342, PC-MA342], Mycostop [Streptomyces sp. K61, SG-K61], Proradix®Agro [Pseudomonas sp. DSMZ13134, PS-DSMZ13134] and fungi (Clonotry [Trichoderma harzianum and Clonostachys rosea, TH+CR], Remedier [T. asperellum ICC012 and T. gamsii ICC080, TA-ICC012+TG-ICC080], Rootshield WP [T. harzianum T22, TH-T22] were tested for efficacy against Fusarium solani f. sp. cucurbitae race 1 (FSC7 strain on zucchini. They were applied to seeds (S, plant growth substrate (PGS and both (S+PGS in a growth chamber experiment, and to PGS, transplantation soil mixture (TSM and both (PGS+TSM in a greenhouse experiment. FSC7 was inoculated in PGS at sowing time in the growth chamber and in TSM at transplant in the greenhouse. In the growth chamber, the most effective products were Cedomon (S and S+PGS treatments, Rootshield (PGS treatment and Proradix (S+PGS treatment, reducing the disease by 39.7, 43.1, 25.8 and 36.4%, respectively. In the greenhouse, all tested products applied to PGS reduced the disease severity and more markedly when applied to PGS+TSM. In the PGS and PGS+TSM treatments, Cedomon was the most effective product showing a disease decrease by 42.4 and 59.5%, respectively. The data obtained in vivo were consistent with the ability of the antagonists to colonize zucchini rhizosphere and with their inhibitory effects on the growth of the pathogen in in vitro assays. The bacteria caused the greatest growth inhibition of FSC7 showing abnormal morphology, while Trichoderma spp. parasitized FSC7 hyphae. Bacteria were the most active in reducing pathogen colony growth through antibiotic metabolites. All antagonists produced exo- and endochitinase enzymes. Trichoderma strains showed greater levels of β-N-acetylhexosaminidase and endochitinase, whereas SG-K61 was the most active producer of chitin 1,4-β-chitobiosidase. These results indicate that the studied bioproducts have potential

  5. Antioxidative effects of pumpkin seed (Cucurbita pepo) protein isolate in CCl4-induced liver injury in low-protein fed rats.

    Science.gov (United States)

    Nkosi, C Z; Opoku, A R; Terblanche, S E

    2006-11-01

    The effects of pumpkin seed (Cucurbita pepo) protein isolate on the plasma activity levels of catalase (CA), superoxide dismutase (SOD), glutathione peroxidase (GSHpx) and total antioxidant capacity (TAC) as well as glucose-6-phosphatase (G6Pase) in liver homogenates and lipid peroxidation (LPO-malondialdehyde-MDA) levels in liver homogenates and liver microsomal fractions against carbon tetrachloride (CCl(4))-induced acute liver injury in low-protein fed Sprague-Dawley rats (Rattus norvegicus) were investigated. A group of male Sprague-Dawley rats maintained on a low-protein diet for 5 days were divided into three subgroups. Two subgroups were injected with carbon tetrachloride and the other group with an equivalent amount of olive oil. Two hours after CCl(4) intoxication one of the two subgroups was administered with pumpkin seed protein isolate and thereafter switched onto a 20% pumpkin seed protein isolate diet. The other two groups of rats were maintained on the low-protein diet for the duration of the investigation. Groups of rats from the different subgroups were killed at 24, 48 and 72 h after their respective treatments. After 5 days on the low-protein diet the activity levels of all the enzymes as well as antioxidant levels were significantly lower than their counterparts on a normal balanced diet. However, a low-protein diet resulted in significantly increased levels of lipid peroxidation. The CCl(4) intoxicated rats responded in a similar way, regarding all the variables investigated, to their counterparts on a low-protein diet. The administration of pumpkin seed protein isolate after CCl(4) intoxication resulted in significantly increased levels of all the variables investigated, with the exception of the lipid peroxidation levels which were significantly decreased. From the results of the present study it is concluded that pumpkin seed protein isolate administration was effective in alleviating the detrimental effects associated with protein

  6. Total body nitrogen and total body carbon as indicators of body protein and body lipids in the melon fly Bactrocera cucurbitae: effects of methoprene, a juvenile hormone analogue, and of diet supplementation with hydrolyzed yeast.

    Science.gov (United States)

    ul Haq, Ihsan; Mayr, Leopold; Teal, P E A; Hendrichs, Jorge; Robinson, Alan S; Stauffer, Christian; Hood-Nowotny, Rebecca

    2010-12-01

    The application of methoprene, and providing access to diet including hydrolyzed yeast, are treatments known to enhance mating success in the male melon fly Bactrocera cucurbitae Coquillett (Diptera: Tephritidae), supporting their use in mass rearing protocols for sterile males in the context of sterile insect technique (SIT) programmes. The objective of the present laboratory study was to investigate the effect of methoprene application and diet supplementation with hydrolyzed yeast (protein) on the turnover of body lipids and protein to confirm the feasibility of their application in melon fly SIT mass-rearing programmes. While females had access to a diet that included hydrolyzed yeast (protein), males were exposed to one of the following treatments: (1) topical application of methoprene and access to diet including protein (M+P+); (2) only diet including protein (M-P+); (3) only methoprene (M+P-) and (4) untreated, only sugar-fed, control males (M-P-). Total body carbon (TBC) and total body nitrogen (TBN) of flies were measured at regular intervals from emergence to 35 days of age for each of the different treatments. Nitrogen assimilation and turnover in the flies were measured using stable isotope ((15)N) dilution techniques. Hydrolyzed yeast incorporation into the diet significantly increased male body weight, TBC and TBN as compared to sugar-fed males. Females had significantly higher body weight, TBC and TBN as compared to all males. TBC and TBN showed age-dependent changes, increasing until the age of sexual maturity and decreasing afterwards in both sexes. Methoprene treatment did not significantly affect TBC or TBN. The progressive increase with age of TBC suggests that lipogenesis occurs in adult male B. cucurbitae, as is the case in other tephritids. Stable isotope dilution was shown to be an effective method for determining N uptake in B. cucurbitae. This technique was used to show that sugar-fed males rely solely on larval N reserves and that the N

  7. Mercury Pollution Characteristics in Tongren Mercury Mining Area,Guizhou Province, China%贵州省铜仁汞矿区汞污染特征研究

    Institute of Scientific and Technical Information of China (English)

    夏吉成; 胡平; 王建旭; 张华; 冯新斌

    2016-01-01

    铜仁汞矿位于铜仁市碧江区云场坪镇,曾是贵州省最大的汞矿之一.为了了解该矿在闭坑后矿区的汞污染特征,采集了矿区的大气、水体、矿渣、土壤和农作物样品,分析了汞的分布特征.结果表明,矿区大气汞浓度为7.29~ 139 ng·m-3、地表水汞浓度为81.6~425×103 ng·L-1、矿渣汞含量为2.79~510 mg·kg-1、土壤汞含量为3,06~2.92× 103 mg· kg-1.可见,大气、水体、矿渣和土壤中的汞含量远高于对照区或国家相关标准.共采集了10种农作物玉米(Zea mays L)、高粱(Chinese sorghum)、枣(Ziziphus jujuba Mill)、梨(Pyrus spp)、茄子(Solanum melongena)、丝瓜(Luffa cylindrica)、西红柿(Lycopersicon esculentum)、南瓜(Cucurbita moschata duchesne)、苦瓜(Balsam pear)和辣椒(Capsicum annuum),除了茄子和南瓜外,其余农作物可食部分的汞含量都高于国家《食品安全国家标准》.综上所述,铜仁汞矿开采和冶炼带来的汞污染已严重影响周边生态环境和食品安全,矿区汞污染不容忽视.

  8. Efeito da farinha de semente de abóbora (Cucurbita maxima, L. sobre o metabolismo glicídico e lipídico em ratos The pumpkin (Cucurbita maxima, L. seed flour effect on the rat glucose and lipid metabolism

    Directory of Open Access Journals (Sweden)

    Priscila Machado de Cerqueira

    2008-04-01

    Full Text Available OBJETIVO: O objetivo desta pesquisa foi avaliar o efeito da farinha de semente de abóbora (Cucurbita maxima, L. sobre o metabolismo glicídico e lipídico em ratos. MÉTODOS: Vinte ratos Wistar machos recém-desmamados, distribuídos em quatro grupos, receberam por 10 dias, rações controle e experimental com farinhas de semente de abóbora integral, peneirada ou residual substituindo 30% do valor total de amido e dextrina da dieta controle. As dietas foram isocalóricas. Foram determinados os macronutrientes e a fibra insolúvel nas farinhas. As dietas tiveram a composição química calculada a partir dos dados dos rótulos dos produtos, da tabela de composição de alimentos e da análise química das farinhas de semente de abóbora. O peso corporal e a ingestão dos animais foram tomados a cada 48 horas. O sangue, coletado por punção cardíaca, teve os níveis de triacilgliceróis, colesterol e glicose analisados por métodos enzimáticos. RESULTADOS: As farinhas de semente de abóbora foram boas fontes de proteínas, lipídeos e, especialmente, fibras alimentares. Os animais tiveram ganho ponderal e ingestão semelhante (p>0,05. Os níveis de glicose e triacilgliceróis foram reduzidos significantemente para os grupos que receberam dietas com farinhas de semente de abóbora integral e peneirada. CONCLUSÃO: Frente às suas propriedades química e funcional, concluiu-se que a farinha de semente de abóbora interferiu no metabolismo do rato diminuindo significantemente os níveis de glicose e triacilgliceróis séricos.OBJECTIVE: The aim of the present research was to evaluate the pumpkin (Cucurbita maxima, L. seed flour effect on the rat glucose and lipid metabolism. METHODS: Twenty recent weaned male Wistar rats, divided in four groups, received for 10 days control and experimental diets containing whole, sifted and residual pumpkin seed flour on the rate of 30% of the total starch and dextrin in the control diet. All diets were

  9. Yield of varieties of Cucurbita pepo preimmunized with mild strains of Papaya ringspot virus - type W and Zucchini yellow mosaic virus Produção de variedades de Cucurbita pepo premunizadas com estirpes fracas do Papaya ringspot virus - type W e do Zucchini yellow mosaic virus

    Directory of Open Access Journals (Sweden)

    Estela Bonilha

    2009-06-01

    Full Text Available Papaya ringspot virus - type W (PRSV-W and Zucchini yellow mosaic virus (ZYMV are the most prevalent viruses in cucurbit crops in Brazil and responsible for frequent yield losses. Diseases caused by these viruses are difficult to control. The objective of this work was to evaluate the effects of mild strains PRSV-W-1 and ZYMV-M on the yield of Cucurbita pepo L. cvs. Samira, Novita Plus, AF 2847, and Yasmin, under plastic greenhouse and field conditions. Plants infected with ZYMV-M and grown in a plastic greenhouse did not exhibit typical leaf symptoms or significant alterations in quantitative and qualitative fruit yield. However, when infected with PRSV-W-1, or PRSV-W-1 + ZYMV-M, the plants exhibited severe leaf mosaic symptoms and reduced fruit quality, although there were no changes in the number and mean fruit weight harvested from these plants. When these plants were infected with PRSV-W-1 and studied simultaneously in the field and plastic greenhouse, intensification of symptoms in the fruits and leaves was more pronounced under the greenhouse conditions. Quantitative yield did not change. Environmental factors seem to influence symptoms induced by PRSV-W-1.O Papaya ringspot virus - type W (PRSV-W e o Zucchini yellow mosaic virus (ZYMV são os vírus predominantes em culturas de cucurbitáceas no Brasil, onde geralmente causam danos significativos na produção. As doenças causadas por ambos os vírus são de difícil controle. O objetivo desse trabalho foi avaliar o efeito das estirpes fracas PRSV-W-1 e ZYMV-M na produção de abobrinha de moita (Cucurbita pepo L. cvs. Samira, Novita Plus, AF 2847, and Yasmin em condições de estufa plástica e de campo. Plantas infectadas com a estirpe ZYMV-M sob condições de estufa plástica não exibiram sintomas foliares típicos da doença e alterações na quantidade e qualidade dos frutos produzidos. No entanto, quando infectadas com a estirpe PRSV-W-1, ou PRSV-W-1 + ZYMV-M, as plantas

  10. Repelência e deterrência na oviposição de Bemisia tabaci biótipo B pelo uso de extratos vegetais em Cucurbita pepo L Repellence and deterrence on oviposition of Bemisia tabaci biotype B by the use of vegetal extracts in Cucurbita pepo L

    Directory of Open Access Journals (Sweden)

    J.P.G.F. Silva

    2012-01-01

    Full Text Available Bemisia tabaci biótipo B é um dos principais insetos-praga na cultura da abobrinha (Cucurbita pepo L.. O manejo dessa mosca-branca tornou-se grande desafio aos agricultores, uma vez que esta apresenta rápida capacidade de desenvolver resistência a diferentes classes de inseticidas. Como alternativa vem sendo investigado o uso de extratos vegetais com atividades inseticida e/ou insetistática, os quais têm revelado resultados promissores no combate a inseto. O presente trabalho teve por objetivo avaliar os possíveis efeitos de sete extratos provenientes de cinco espécies vegetais, Ruta graveolens L. (folhas, Azadirachta indica A. Juss. (folhas + ramos, Trichilia pallida Swartz (ramos, T. pallida (folhas, A. indica (amêndoas, Chenopodium ambrosioides L. (inflorescências + ramos+ folhas e Mentha pulegium L. (folhas, sobre Bemisia tabaci biótipo B em abobrinha, por meio de testes de repelência e deterrência para oviposição. Em teste com chance de escolha, avaliou-se a atratividade e determinou-se o índice de repelência após 6, 24 e 48 horas da aplicação dos extratos. A oviposição foi verificada após a terceira contagem do número de adultos. O extrato à base de folhas de M. pulegium mostrou repelência e deterrência à oviposição de B. tabaci biótipo B, podendo ser recomendado como alternativa para o manejo do inseto.Bemisia tabaci biotype B is one of the main pests in squash (Cucurbita pepo L.. The management of this whitefly has become a major challenge to growers, since they have the ability to quickly develop resistance to different classes of insecticides. Alternatively, the use of plant extracts with insecticide and/or insectistatic activities has been investigated, showing promising results for the whitefly control. The present study evaluated the possible effects of seven extracts from five plant species, Ruta graveolens L. (leaves, Azadirachta indica A. Juss. (leaves + branches, Trichilia pallida Swartz (branches

  11. Field trials of solid triple lure (trimedlure, methyl eugenol, raspberry ketone, and DDVP) dispensers for detection and male annihilation of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae (Diptera: Tephritidae) in Hawaii.

    Science.gov (United States)

    Vargas, Roger I; Souder, Steven K; Mackey, Bruce; Cook, Peter; Morse, Joseph G; Stark, John D

    2012-10-01

    Solid Mallet TMR (trimedlure [TML], methyl eugenol [ME], raspberry ketone [RK]) wafers and Mallet CMR (ceralure, ME, RK, benzyl acetate) wafers impregnated with DDVP (2,2-dichlorovinyl dimethyl phosphate) insecticide were measured in traps as potential detection and male annihilation technique (MAT) devices. Comparisons were made with 1) liquid lure and insecticide formulations, 2) solid cones and plugs with an insecticidal strip, and 3) solid single and double lure wafers with DDVP for captures of Mediterranean fruit fly, Ceratitis capitata (Wiedemann); oriental fruit fly, Bactrocera dorsalis Hendel; and melon fly, B. cucurbitae Coquillett. Bucket and Jackson traps were tested in a coffee plantation near Eleele, Kauai Island, HI (trials at high populations) and avocado orchards near Kona, HI Island, HI (trials at low populations). Captures of all three species with Mallet TMR were not different from Mallet CMR; therefore, subsequent experiments did not include Mallet CMR because of higher production costs. In MAT trials near Eleele, HI captures in AWPM traps with Mallet TMR wafers were equal to any other solid lure (single or double) except the Mallet ME wafer. In survey trials near Kona, captures of C. capitata, B. cucurbitae, and B. dorsalis with Mallet TMR wafers were equal to those for the standard TML, ME, and C-L traps used in FL and CA. A solid Mallet TMR wafer is safer, more convenient to handle, and may be used in place of several individual lure and trap systems, potentially reducing costs of large survey and detection programs in Florida and California, and MAT programs in Hawaii.

  12. CRITERIOS DE SELECCIÓN EN LOS PROCESOS DE MANIPULACIÓN VEGETAL: EL APORTE DE LA ETNOBOTÁNICA A LA INTERPRETACIÓN DE RESTOS ARQUEOBOTÁNICOS DE CUCURBITA SP

    Directory of Open Access Journals (Sweden)

    Verónica S. Lema

    2009-01-01

    Full Text Available En este trabajo se realiza el estudio etnobotánico-etnoarqueológico de diversos cultivos locales de Cucurbita maxima subsp. maxima, Cucurbitaceae. El objetivo es comprobar si los caracteres cuantitativos y cualitativos de las semillas procedentes de los mencionados cultivos, así como también de plantas resultantes de prácticas de cultivo o cuidado, se agrupan o no en morfotipos diferenciales que reflejen materialmente la diversidad localmente reconocida y los distintos comportamientos involucrados. La generación de dichos morfotipos se propone como una vía para explorar si es factible reconocer cultivares locales y distintas formas de relación hombre - planta en muestras arqueobotánicas. Las plantas domesticadas son el producto de la puesta en práctica de criterios de selección socialmente construidos y por lo tanto susceptibles de constituir morfotipos diferenciales que operen como portadores de significados sociales. De allí que podamos hablar de una etnobotánica etnoarqueológica generadora de modelos a contrastar en el trabajo paleoetnobotánico. Se aplicaron los descriptores morfológicos macroscópicos comúnmente empleados en paleoetnobotánica y arqueobotánica para el estudio de semillas de Cucurbita sp., así como también un conjunto nuevo de descriptores micro morfológicos. Se constató que los caracteres cuantitativos micro morfológicos son los que mejor sirven para crear morfotipos que se corresponden con los cultivares localmente reconocidos y con las prácticas de cultivo o cuidado ejercidas sobre las poblaciones vegetales.

  13. Cultivar-Based Introgression Mapping Reveals Wild Species-Derived Pm-0, the Major Powdery Mildew Resistance Locus in Squash.

    Science.gov (United States)

    Holdsworth, William L; LaPlant, Kyle E; Bell, Duane C; Jahn, Molly M; Mazourek, Michael

    2016-01-01

    Powdery mildew is a major fungal disease on squash and pumpkin (Cucurbita spp.) in the US and throughout the world. Genetic resistance to the disease is not known to occur naturally within Cucurbita pepo and only infrequently in Cucurbita moschata, but has been achieved in both species through the introgression of a major resistance gene from the wild species Cucurbita okeechobeensis subsp. martinezii. At present, this gene, Pm-0, is used extensively in breeding, and is found in nearly all powdery mildew-resistant C. pepo and C. moschata commercial cultivars. In this study, we mapped C. okeechobeensis subsp. martinezii-derived single nucleotide polymorphism (SNP) alleles in a set of taxonomically and morphologically diverse and resistant C. pepo and C. moschata cultivars bred at Cornell University that, by common possession of Pm-0, form a shared-trait introgression panel. High marker density was achieved using genotyping-by-sequencing, which yielded over 50,000 de novo SNP markers in each of the three Cucurbita species genotyped. A single 516.4 kb wild-derived introgression was present in all of the resistant cultivars and absent in a diverse set of heirlooms that predated the Pm-0 introgression. The contribution of this interval to powdery mildew resistance was confirmed by association mapping in a C. pepo cultivar panel that included the Cornell lines, heirlooms, and 68 additional C. pepo cultivars and with an independent F2 population derived from C. okeechobeensis subsp. martinezii x C. moschata. The interval was refined to a final candidate interval of 76.4 kb and CAPS markers were developed inside this interval to facilitate marker-assisted selection.

  14. 自毒物质对黄瓜和南瓜种子萌芽的影响%Effects of Toxic Substances on Germination of Cucumber and Pumpkin Seeds

    Institute of Scientific and Technical Information of China (English)

    乔永旭; 张彦; 张永平; 陈超; 刘海英

    2015-01-01

    Effects of benzoic acid on seed germination rate,root length,root diameter,root curvature and seed viability of cucumber and pumpkin was investigated.The results showed that with the increasing of the benzoic acid concentration,seed germination rate,radicle length and seed viability declined,and Cucumis sativas decreased the most obvious,followed by cucurbita moschata,finally was cucurbita f1cifolia.The bending angle of seeds and the root diameter increased,Cucumis sativas increased the most,followed by cucurbita moschata,finally was cucurbita f1cifolia.So benzoic acid does more harmful to Cucumis sativas,then is cucurbita moschata,finally is cucurbita f1cifolia.%以黄瓜、黑籽南瓜和白籽南瓜种子为试材,研究不同浓度的苯甲酸对种子露白率、胚根长度、胚根弯曲度、胚根直径及种子活率的影响.结果表明:随着苯甲酸浓度的增加,种子露白率、胚根长度和种子活率均呈下降趋势,且黄瓜降低的最明显,其次是白籽南瓜,最后是黑籽南瓜;种子的弯曲度和胚根直径则呈现上升趋势,黄瓜增加的最明显,其次是白籽南瓜,最后是黑籽南瓜.总之,黑籽南瓜受到苯甲酸的危害程度低,其次是白籽南瓜,受到苯甲酸危害最大的是黄瓜.

  15. 不同砧木嫁接对西瓜生长发育及产量的影响%Effects of Different Rootstock on the Yield and Development of Citrullus lanatus var.lanatus

    Institute of Scientific and Technical Information of China (English)

    常高正; 张慎璞; 杨红丽; 赵卫星; 徐小利; 李晓慧; 张四普; 杨帆

    2009-01-01

    研究了豫园砧木王(Citrullus lanatus var.citroides cv.Yuyuanzhenmu)、超丰F1(Lagenaria siceraria cv.Chaofeng F1)、新土佐(Cucurbita maxima xC.moschata,Sintozwa)3个西瓜砧木嫁接对西瓜(Citrullus lanatus var.lanatus)生长发育、根系的分布及产量的影响.结果表明,3种不同砧木西瓜嫁接苗与自根苗相比,根系发达,茎叶生长旺盛,且有增产作用.其中豫园砧木王和新土佐砧木的嫁接效果最好,其能明显增强嫁接苗的长势,可分别使西瓜增产21.4%和25.0%.因此,建议选择豫园砧木王和新土佐嫁接西瓜为好,以达到丰产的目的.%Effects of Citrullus lanatus var. citroides cv. Yuyuanzhenmu,Lagenaria siceraria cv. Chaofeng Fl and Sintozwa (Cucurbita maxima ×C. moschata) on the development,distinction of the roots and the yield of C. lanatus var. lanatus were studied. The results showed that,compared with non-grafted seedling,the roots and shoots of the grafted seedlings with three rootstocks developed faster,and the yield increased. Of the three rootstocks,the grafting effect of C. lanatus var. citroides Yuyuanzhenmu and Sintozwa (Cucurbita maxima ×C. moschata) was better. The growth vigor and yields of grafted seedlings increased by 21.4% and 25.0% respectively. So,to grain high production,C. lanatus var. citroides Yuyuanzhenmu and Sintozwa (Cucurbita maxima ×C. moschata) were recommended to be used as rootstocks of C. lanatus var. lanatus.

  16. Evaluating Pumpkin Properly and Furthering Pumpkin Industry Correctly%科学评价南瓜,促进南瓜产业健康发展

    Institute of Scientific and Technical Information of China (English)

    刘宜生; 王长林

    2005-01-01

    从园艺学的分类来看,南瓜包括南瓜(中国南瓜)(Cucurbita moschata D.)、笋瓜(印度南瓜)(C.maxima D.)、西葫芦(美洲南瓜)(C.pepo L.)、黑籽南瓜(C.ficifolia B.)和灰籽南瓜(C.mixta P.)等五个栽培种。

  17. 南瓜藤作秀宴会厅

    Institute of Scientific and Technical Information of China (English)

    冯启浩

    2004-01-01

    @@ 南瓜,又称番瓜,沪语中称作饭瓜者众,顾名思义,昔时家境贫困者曾以它作为主食之一.它在科学分类学上属于葫芦科,南瓜属中的"南瓜"者.若用全世界公认的通用大名则用拉丁文写出该是Cucurbita moschata.

  18. FLOWERING LOCUS T protein may act as the long-distance florigenic signal in the cucurbits.

    Science.gov (United States)

    Lin, Ming-Kuem; Belanger, Helene; Lee, Young-Jin; Varkonyi-Gasic, Erika; Taoka, Ken-Ichiro; Miura, Eriko; Xoconostle-Cázares, Beatriz; Gendler, Karla; Jorgensen, Richard A; Phinney, Brett; Lough, Tony J; Lucas, William J

    2007-05-01

    Cucurbita moschata, a cucurbit species responsive to inductive short-day (SD) photoperiods, and Zucchini yellow mosaic virus (ZYMV) were used to test whether long-distance movement of FLOWERING LOCUS T (FT) mRNA or FT is required for floral induction. Ectopic expression of FT by ZYMV was highly effective in mediating floral induction of long-day (LD)-treated plants. Moreover, the infection zone of ZYMV was far removed from floral meristems, suggesting that FT transcripts do not function as the florigenic signal in this system. Heterografting demonstrated efficient transmission of a florigenic signal from flowering Cucurbita maxima stocks to LD-grown C. moschata scions. Real-time RT-PCR performed on phloem sap collected from C. maxima stocks detected no FT transcripts, whereas mass spectrometry of phloem sap proteins revealed the presence of Cm-FTL1 and Cm-FTL2. Importantly, studies on LD- and SD-treated C. moschata plants established that Cmo-FTL1 and Cmo-FTL2 are regulated by photoperiod at the level of movement into the phloem and not by transcription. Finally, mass spectrometry of florally induced heterografted C. moschata scions revealed that C. maxima FT, but not FT mRNA, crossed the graft union in the phloem translocation stream. Collectively, these studies are consistent with FT functioning as a component of the florigenic signaling system in the cucurbits.

  19. [Effects of NaCl stress on cation contents in seedlings of two pumpkin varieties].

    Science.gov (United States)

    Wang, Ran; Chen, Gui-Lin; Song, Wei; Lü, Gui-Yun; Liang, Jing; Li, Wei-Xin

    2006-02-01

    Effects of different concentration NaCl stress on cation contents of pumpkin cultivars (Cucurbita ficifolia and Cucurbita moschata) usually used as rootstocks of cucumber were studied. The results showed that, in both cultivars treated with 150, 300 and 500 mmol/L NaCl for 7 d, increasing salinity caused in increasing retardation of shoot and root growth, and reduction of shoot relative water content (Table 1). With increasing NaCl concentration, Na(+) content increased while K(+) content decreased distinctly in roots, stems and leaves of both cultivars. And Na(+) and K(+) contents in different organs were in the orders roots>stems>leaves and stems>leaves>roots respectively (Fig.3). There were less Na(+) in root, stem and leaf (Fig.3), more free proline and soluble sugar in leaves (Fig.1, 2), and higher shoot relative water content (Table 1) in C. ficifolia than in C. moschata. So there are differences between C. ficifolia and C. moschata in osmotic adjustment mechanisms and selective absorption and transportation of different cations, and the salt tolerance of C. ficifolia is higher than C. moschata.

  20. The Evaluation of Dipeptidyl Peptidase (DPP)-IV, α-Glucosidase and Angiotensin Converting Enzyme (ACE) Inhibitory Activities of Whey Proteins Hydrolyzed with Serine Protease Isolated from Asian Pumpkin (Cucurbita ficifolia).

    Science.gov (United States)

    Konrad, Babij; Anna, Dąbrowska; Marek, Szołtysik; Marta, Pokora; Aleksandra, Zambrowicz; Józefa, Chrzanowska

    2014-01-01

    In the present study, whey protein concentrate (WPC-80) and β-lactoglobulin were hydrolyzed with a noncommercial serine protease isolated from Asian pumpkin (Cucurbita ficifolia). Hydrolysates were further fractionated by ultrafiltration using membranes with cut-offs equal 3 and 10 kDa. Peptide fractions of molecular weight lower than 3 and 3-10 kDa were further subjected to the RP-HPLC. Separated preparations were investigated for their potential as the natural inhibitors of dipeptidyl peptidase (DPP-IV), α-glucosidase and angiotensin converting enzyme (ACE). WPC-80 hydrolysate showed higher inhibitory activities against the three tested enzymes than β-lactoglobulin hydrolysate. Especially high biological activities were exhibited by peptide fractions of molecular weight lower than 3 kDa, with ACE IC50 <0.64 mg/mL and DPP-IV IC50 <0.55 mg/mL. This study suggests that peptides generated from whey proteins may support postprandial glycemia regulation and blood pressure maintenance, and could be used as functional food ingredients in the diet of patients with type 2 diabetes.

  1. 2D NMR对苦味西葫芦果实中的一个三萜皂苷进行结构解析%Structure Determination of A Cucurbitacin Glycoside Extracted from Cucurbita pepo cv Dayangua by 2D NMR

    Institute of Scientific and Technical Information of China (English)

    王大成; 葛杉; 高慧媛; 蔡辉; 吴斌; 吴立军; 邓旭明

    2005-01-01

    通过核磁共振谱并结合文献对一个三萜皂苷葫芦素E 2-O-β-D-葡萄糖苷进行结构解析.通过2D NMR(1H-1H COSY、HMQC和HMBC)进行了NMR全归属.%A triterpene saponin was extracted from traditional Chinese medicine Cucurbita pepo cv Dayangua. Its structure was determined by 2D NMR. Complete assignment of the 1H and 13C chemical shifts for the compound was obtained. The structure was elucidated to be cucurbitacin E 2-O-b-D-glucoside.

  2. Antalya İli ve İlçelerindeki Örtüaltı Hıyar (Cucumis sativus L. ve Kabak (Cucurbita pepo L. Üretim Alanlarında Viral Etmenlerin Saptanması

    Directory of Open Access Journals (Sweden)

    Ahmet ÇAT

    2016-04-01

    Full Text Available Bu çalışma, 2014-2015 yılları arasında Antalya ili ve ilçelerinde örtüaltı hıyar (Cucumis sativus L. ve kabak  (Cucurbita pepo L. üretim alanlarında ZYMV (Zucchini yellow mosaic virus, PRSV (Papaya ring spot virus, SqMV (Squash mosaic virus ve CMV (Cucumber mosaic virus’nün varlığının, serolojik, ve biyolojik yöntemlerle saptanması ve toplanan örneklerdeki yaygınlığının ortaya konulması amacıyla 2014-2015 yılları arasında yürütülmüştür. Örtüaltı hıyar ve kabak üretim alanlarından alınan ve virüs şüphesi duyulan 455 yaprak ve meyve örneğinin hepsi DAS-ELISA ile testlenmiştir. Teslenen 455 örneğin 346 adedininde (%76 bir ve daha fazla virüs ile enfekteli olduğu belirlenmiştir. ELISA testleri sonucunda pozitif reaksiyon veren bitkilerden alınan dokular mekanik inokulasyon çalışmalarında kullanılmıştır.  İndikatör bitkiler üzerinde 15-30 gün gibi bir sürede belirti gözlenmiştir.

  3. Toxicity Determination of Several Insecticides and Their Mixtures to Bactrocera (Zeugodacus) cucurbitae ( Coquillett)%几种杀虫剂及其混配对瓜实蝇成虫的毒力测定

    Institute of Scientific and Technical Information of China (English)

    刘奎; 付步礼; 邱海燕; 符悦冠

    2011-01-01

    采用微量点滴法,测定了毒死蜱、甲维盐、敌敌畏、顺式氯氰菊酯等4种杀虫剂对瓜实蝇成虫的毒力和二元混配的联合毒力.结果表明:80%敌敌畏乳油(EC)的LD50最小,为1.683 9μg·g-1;100g·L-1顺式氯氰菊酯EC的LD50最大,为8.877 0μ g·g-1.供试杀虫剂毒性大小依次为:80%敌敌畏EC>5%甲维盐微乳剂(ME) >480g·L-1毒死蜱EC> 100 g·L-1顺式氯氰菊酯EC.敌敌畏与甲维盐(1∶1)混配的增效作用最强,共毒系数为426.%Four insecticides toxicities to adult of Bactrocera (Zeugodacus ) cucurbitae ( Coquillett) and their co-efficient ratio were determined by micro drop method. The results showed that LD50 of 80 % dichlorvos EC was minimum for 1.683 9 μg ? G-1 and alpha-cypermethrin EC was the largest for 8.877 0 μg ? G-1. The toxicity order was 80 % dichlorvos EC>5 % emamectin-benzoate ME > 480 g ? L-1 chlorpyrifos EC> 100 g ? L-1 alpha-cypermethrin EC. The co-efficient ratio of the mixture of dichlorvos and emamectin-benzoate (1:1) was 426.

  4. 不同温度对西葫芦果实冷害及生理变化的影响%Effects of Different Temperature on Chilling Injury and Physiological Changes during Storage of Cucurbita pepo

    Institute of Scientific and Technical Information of China (English)

    王清; 杨娜; 刘凤娟; 高丽朴

    2012-01-01

    通过试验确定西葫芦果实在不同温度下贮藏的安全期,并分析不同冷藏温度下其营养品质和生理生化指标的变化.结果表明,在单纯5、8、10℃低温处理下冷害出现的时间分别为处理后的7、5、6d.但5、8、10℃处理分别冷藏3、4、5d后,再在20℃环境的货架上1d就会表现出冷害;冷害对西葫芦果实可溶性蛋白含量影响不大,但会降低可溶性固形物的含量与过氧化氢酶活性,影响细胞膜透性、丙二醛和过氧化氢含量.%To ensure the safety period of summer squash (Cucurbita pepo L.) at different temperature, the changes of nutrition quality and physiological and biochemical indexes of summer squash at different cold storage temperature were studied. The results showed that the chilling injury of summer squash occurred 7, 5, 6d after being stored at 5, 8, 10 ℃, respectively. But chilling injury would occur on shelf just in 1 d at 20 ℃ after being stored at 5, 8, 10 ℃ for 3, 4, 5d, respectively. The effect of chilling injury on the soluble protein content of summer squash was limited. However, the soluble solid content and catalase activity were reduced; and the cell membrane permeability, content of Malondialdehyde and Hydrogen peroxide were influenced.

  5. 橘小实蝇、瓜实蝇和南亚果实蝇人工饲料的优化%Optimization of artificial diets for mass rearing of Bactrocera dorsalis,Bactrocera cucurbitae and Bactrocera tau

    Institute of Scientific and Technical Information of China (English)

    林明光; 汪兴鉴; 张艳; 孙蕊芬; 曾玲

    2013-01-01

    在温度25 ~28℃、相对湿度70%~75%和光照周期L:D=14∶10条件下进行了人工大量饲养橘小实蝇Bactrocera dorsalis (Hendel)和瓜实蝇B.cucurbitae (Coquillett)成虫人工饲料配方的筛选试验.结果显示,1∶2重量比例混合的蔗糖和啤酒酵母是饲养这2种果实蝇的最佳成虫人工饲料,用其饲养的单雌产卵量、产卵期和孵化率分别为424.16 ~ 445.75粒,30.90 ~31.87 d,74.60% ~ 75.40%.同时,对18种由不同配方配制成的幼虫人工饲料饲养橘小实蝇、瓜实蝇和南亚果实蝇B.tau (Walker)的效果进行了比较.结果表明,玉米和麦麸作为饲料的介质优于麦片和麦麸.橘小实蝇幼虫人工饲料的优化配方为:玉米+麦麸(125 g+25 g),蔗糖25 g,啤酒酵母25 g,对羟基苯甲酸甲酯0.9g,1 mol/L盐酸4 mL,纸巾4 g,自来水300 mL;用其幼虫人工饲料饲养该虫的生物学参数包括子代孵化率、化蛹率、羽化率和平均蛹重分别为81.17% ±0.05%,96.41% ±0.02%,94.85%±0.01%与(19.40±0.08) mg.而瓜实蝇和南亚果实蝇幼虫人工饲料的优化配方为:玉米+麦麸(100 g+50 g),蔗糖30 g,啤酒酵母25 g,以及一定量的其他组分(同上);用其饲料饲养这2种果实蝇的相关参数:子代孵化率、化蛹率、羽化率和平均蛹重分别为78.50%±0.04%与76.96%±0.12%,95.73% ±0.03%与94.69% ±0.02%,94.57%±0.02%与95.82%±0.03%,(18.62±0.23) mg与(22.83±1.38) mg.试验证实,优化后的成、幼虫人工饲料具有饲养效果好、方法简便,配方材料来源广泛和价格低廉等优点,可用于室内人工大量饲养上述3种果实蝇属害虫.%Artificial diets for adult Bactrocera dorsalis (Hendel) and B.cucurbitae (Coquillett) were evaluated in the laboratory at 25-28℃,70%-75% RH and a L∶ D ratio of 14∶ 10.A mixture of 1 part sucrose to 2 parts beer yeast by weight was the optimum artificial diet for adults of these two species.The egg quantity

  6. INFLUENCE OF ROOTSTOCKS ON Fusarium WILT, NEMATODE INFESTATION, YIELD AND FRUIT QUALITY IN WATERMELON PRODUCTION

    Directory of Open Access Journals (Sweden)

    Juan Carlos Álvarez-Hernández

    2015-08-01

    Full Text Available Cucurbita maxima x Cucurbita moschata rootstock are used to prevent infection with Fusarium oxysporum f. sp. niveum in watermelon production; however, this rootstock is not effective against nematode attack. Because of their vigor, the grafted plants can be planted at lower plant densities than the non-grafted plants. The tolerance to Fusarium oxysporum f. sp. niveum and Meloidogyne incognita was assessed in watermelon plants grafted onto a hybrid of Citrullus lanatus cv Robusta or the Cucurbita maxima x Cucurbita moschata cv Super Shintoza rootstocks. The densities of plants were 2083 and 4166 plants ha-1. Non-grafted watermelons were the controls. The Crunchy Red and Sangría watermelon cultivars were used as the scions, it the latter as a pollinator. The experiments were performed for two production cycles in soils infested with Fusarium oxysporum f. sp. niveum and Meloidogyne incognita. The incidence of Fusarium oxysporum f. sp. niveum was significantly greater in the non-grafted than in the grafted plants. The grafted plants presented similar resistance to Fusarium regardless of the rootstock. The root-knot galling index for Meloidogyne incognita was significantly lower in plants grafted onto Citrullus lanatus cv Robusta than onto the other rootstock. The yields of plants grafted onto Citrullus lanatus cv Robusta grown at both plant densities were significantly higher than in the other treatments.

  7. Characteristics of organic acids in the fruit of different pumpkin species.

    Science.gov (United States)

    Nawirska-Olszańska, Agnieszka; Biesiada, Anita; Sokół-Łętowska, Anna; Kucharska, Alicja Z

    2014-04-01

    The aim of the research was to determine the composition of organic acids in fruit of different cultivars of three pumpkin species. The amount of acids immediately after fruit harvest and after 3 months of storage was compared. The content of organic acids in the examined pumpkin cultivars was assayed using the method of high performance liquid chromatography (HPLC). Three organic acids (citric acid, malic acid, and fumaric acid) were identified in the cultivars, whose content considerably varied depending on a cultivar. Three-month storage resulted in decreased content of the acids in the case of cultivars belonging to Cucurbita maxima and Cucurbita pepo species, while a slight increase was recorded for Cucurbita moschata species.

  8. Reassessing Coxcatlan Cave and the early history of domesticated plants in Mesoamerica.

    Science.gov (United States)

    Smith, Bruce D

    2005-07-05

    Reanalysis and direct accelerator mass spectrometry radiocarbon dating of the cucurbit assemblage from Coxcatlan Cave provide information on the timing and sequence of the initial appearance of three domesticated plants in the Tehuacán Valley (Puebla, Mexico) and allow reassessment of the overall temporal context of plant domestication in Mexico. Cucurbita pepo is the earliest documented domesticate in the cave, dating to 7,920 calibrated calendrical (cal) years B.P. The bottle gourd (Lagenaria siceraria) is dated at 7,200 cal years B.P. Cucurbita argyrosperma does not appear until 2,065 cal years B.P. The earlier identification of Cucurbita moschata specimens is not confirmed. Seventy-one radiocarbon dates, including 23 accelerator mass spectrometry dates on cucurbits, provide ample evidence of postdepositional vertical displacement of organic materials in the western half of Coxcatlan Cave, but they also indicate that the eastern half of the cave was largely undisturbed.

  9. 促性腺激素释放激素基因(GnRH)和生长激素基因(GH)对番鸭产蛋性能的遗传效应分析%Genetic Effects of Gonadotropin-releasing Hormone (GnRH) and Growth Hormone (GH) Genes on the Egg Performance in Muscovy Duck(Cairina moschata)

    Institute of Scientific and Technical Information of China (English)

    吴旭; 严美姣; 刘丽平; 连森阳; 傅星源; 王光瑛; 李昂

    2012-01-01

    Gonadotropin-releasing hormone (GnRH) and growth hormone (GH), which control the reproductive performance of animal, are secreted by hypothalamus pituitary gonadal axis and GH/IGF axis. The single nucleotide polymorphism of GnRH and GH genes was detected for the association with egg performance in white muscovy duck (Cairina moschata) RF line which came from Putian Guangdong Wenshi Poultry Co., Ltd.. PCR-SSCP method was used for identification of genotypes. The results showed that three genotypes AA, AB and BB were found in GnRH gene and a mutation G→A was found by DNA sequencing. Three genotypes CC, CD and DD were found in GH gene and a mutation A→G was found by DNA sequencing. Chi-square test indicated that the two polymorphism sites fitted Hardy-Weinberg equilibrium (P>0.05).The frequency of A/B alleles in thepopulation was for GnRH 0.675 and 0.325, and for GH 0.667 and 0.333. GnRH genotype had been showed a significant difference in the egg number of 300 d (P0.05). The general linear model (GLM) analysis results showed that both of GnRH and GH genes showed mainly in the pattern of additive effect and all the dominant effects were not significant. The additive effect of GnRH gene in the egg number of 300 d and the highest clutch days were -3.53 and -3.33, respectively. The additive effect of GH gene in the highest clutch days was -2.44. It implies that the GnRH and GH genes can be candidate genes locus or linked mark to the major gene, which affect the laying traits significantly in Muscovy duck.%促性腺激素释放激素(GnRH)和生长激素(GH)分别是下丘脑-垂体-性腺轴(HPG)与GH/IGF轴分泌的激素,共同参与调控动物的繁殖性能.本研究以白羽番鸭(Cairina moschata)RF系为实验材料,采用PCR-SSCP技术进行基因分型,同时建立适合的线性统计模型,分析基因型与产蛋性能的关联性.结果表明,GnRH基因5’调控区检测到3种基因型AA/AB/BB,经测序比对AA型与BB型相比发

  10. cDNA Cloning of Vasoactice Intestinal Peptide Receptor-1 Gene (VIPR-1) and Its Expression Characteristics in Tissues of Black Muscovy Duck ( Cairina moschata)%黑番鸭血管活性肠肽受体-1基因(VIPR-1)的cDNA克隆及其组织表达特性分析

    Institute of Scientific and Technical Information of China (English)

    郑嫩珠; 陈晓燕; 陈晖; 朱志明; 缪中纬; 辛清武; 王正朝; 卢立志

    2012-01-01

    最近的研究表明,血管活性肠肽(VIP)与家禽的就巢习性密切相关.本研究采用反转录PCR方法从黑番鸭(Cairina moschata)母鸭下丘脑组织中克隆了血管活性肠肽受体(VIPR-1)基因的cDNA序列,长度为1125bp,编码355个氨基酸(GenBank登录号:JN625215).序列比对结果表明,该序列与家禽(鸡、火鸡、鹌鹑)和哺乳动物(人、小鼠、猪、牛)的基因序列分别有93%~94%和69%~71%的同源性,而相应氨基酸序列的同源性分别为96%和70%~72%.荧光定量PCR结果发现,黑番鸭VIPR-1基因的表达量在产蛋期、就巢期和休产期差异显著(P<0.05)或极显著(P<0.01),就巢期表达量最高,休产期次之,产蛋期表达量最低,表明VIPR-1基因与繁殖阶段变化密切相关;对不同组织VIPR-1的表达量分析发现,VIPR-1在垂体、下丘脑和卵巢中均有表达,其中垂体最多,其次是下丘脑,卵巢中的表达量最低,差异极显著(P<0.01).研究结果提示,VIPR-1基因具有高度保守性,参与下丘脑-垂体-卵巢轴(特别是垂体)对黑番鸭就巢行为的调控.%Recent studies have already indicated that vasoactice intestinal peptide (VIP) is closely related to the habit of birds' nesting. In this study, we first cloned VIP receptor gene and analyzed its characteristics of expression in different tissues of muscovy duck. Reversed transcript PCR was used to clone vasoactice intestinal peptide receptor-1 (VIPR-1) gene from the hypothalamus of black muscovy ducks(Cairirna moschata), the cDNA sequence of this gene consisted of 1 125 nucleotides, which coded 335 amino acids (GenBank accession No. JN625215). The sequence of VIPR-1 was highly conserved in avian species. The muscovy duck VJPR-] shared 96% amino acid identity to that of chicken (Gallus gallus), turkey (Meleagris gallopavo), and quail (Coturnix japonica), while it shared only 70%~72% amino acid identing to that of some mammalians like human(Homo sapiens), mice

  11. 黄瓜、西瓜和南瓜EIN3基因片段的克隆与序列分析%Cloning and Sequence of EIN3 Gene from Cucumis sativus, Citrullus lanatus and Cucurbita maxima

    Institute of Scientific and Technical Information of China (English)

    陈惠明; 卢向阳; 许亮; 许勇; 张海英; 刘晓虹

    2007-01-01

    EIN3(ethylene insensitive 3)位于细胞核的核蛋白,为乙烯信号转导的下游调控基因,根据GenBank植物EIN3基因家族的保守序列设计了1对引物,以黄瓜(Cucumis sativus)、西瓜(Citrullus lanatus)和南瓜(Cucurbita maxima)总DNA为模板PCR扩增到3个725 bp的基因片段CsEIN3、ClEIN3和CmEIN3,并提交GenBank,登录号分别为AY973275、DQ023225和DQ023224.将片段序列在NCBI数据库中Blastn同源搜寻,显示151条有同源性的序列全部是EIN3基因.NCBI网站的ORF(open reading frame)Finder找到正确的开放式阅读框,翻译成为氨基酸序列,对CsEIN3、ClEIN3及CmEIN3氨基酸序列在NCBI网站进行Blastp比对,在大分子结构数据库(molecular modelling database,MMDB)搜索,3个推测蛋白保守结构域三级结构与拟南芥EIN3的DNA结合域(MMDB:30598;PDB:1WIJ)完全相同,系统进化分析表明,黄瓜、西瓜和南瓜与双子叶植物甜瓜、绿豆、蒺藜状苜蓿、烟草、番茄、拟南芥及单子叶水稻、桃红蝴蝶兰等的EIN3家族成员都有较高的相似性.

  12. 印度南瓜梭形果突变体的发现及遗传分析%Discovery and Genetic Analysis of a Fusiform Fruit Mutant in Winter Squash (Cucurbita Maxima Duch.)

    Institute of Scientific and Technical Information of China (English)

    马海龙; 智海英; 韩红艳; 岳青; 梁燕平

    2012-01-01

    A fusiform fruit mutant HL-fu was found from inbred line HL of Cucurbita maxima. There was obvious biological characteristic variation on a lot of organs of the mutant, such as flower, fruit, stem. The residual degree of the stamen from female flower and the pistil from male flower of the mutant were more than the wild type one. Its fruit was fusiform, it was five times on the fruit shape index of the mutant than the wild type one. The arrangement status of the mutant internode was long short alternative distribution, the phyllotaxis was similar to the opposite phyllotaxis. The reciprocal cross F, was constructed by crossing mutant HL-fu and wild type HL, the genetics of the groups of cross F2, BC1 ( P1 ) F1, and BC, ( P2) F1 were analyzed. We speculated that the variation type was recessive mult-effect single gene mutant, the mutant character was controlled by a pair of recessive nuclear genera. We discussed the potential value of fusiform fruit mutant on the pumpkin breeding and the basic study.%从印度南瓜自交系HL中发现了一个梭形果突变体HL-fu.该突变体的花、果、茎等多器官的生物学特性同时发生了明显变异:雌花及雄花花器中异性花蕊的残存度较野生型高;果实为梭形,果形指数达野生型5倍;节间呈现长短交替分布的排列状态,叶序类似于对生.以突变体HL-fu与野生型HL杂交分别构建了正、反交F1,正交F2,BC1(P1)F1、BC1( P2)F1群体进行遗传分析,推测该变异类型为隐性多效单基因突变,突变性状受1对隐性核基因fu控制.探讨了梭形果突变体在南瓜育种及相关基础研究方面的潜在价值.

  13. 西葫芦单倍体诱导及分子检测鉴定%In vitro Induction of Haploid Plants in Squash (Cucurbita pepo L.)and Selection by Molecular Markers

    Institute of Scientific and Technical Information of China (English)

    苏敏; 张国裕; 赵尊练; 张帆; 姜立纲; 李海真

    2011-01-01

    以3种基因型西葫芦为试材,探讨不同基因型与花粉辐照剂量(25~400 Gy)对西葫芦单倍体植株诱导的影响.结果表明,基因型与辐照剂量对植株坐果率和单倍体诱导率均有显著影响.辐照剂量率2 Gy/min,剂量125~250 Gy比较适宜西葫芦单倍体的诱导,获得的再生植株较多,有利于单倍体植株的产生.单倍体植株主要由点状胚与箭形胚发育而来.基因型330的单倍体诱导率为5.08%,基因型331的为7.40%,而基因型332未能获得单倍体植株.利用特异的EST-SSR标记快速对再生植株中的单倍体或自然加倍单倍体植株进行鉴定,并通过保卫细胞叶绿体计数法及流式细胞仪验证SSR标记分析结果的可靠性,确定植株染色体倍性水平.西葫芦单倍体植株生长势弱,叶片较小,花粉败育.%The influence of pollen irradiation and plant genotype on the production of in vitro haploid plants from in situ induced haploid embryos was investigated in squash (Cucurbita pepo L. ). Pollen was irradiated at different gamma-ray doses (25 - 400 Gray). Production of in vitro haploid plantlets was influenced by irradiation dose, genotype, embryo type and embryo stage. Embryos were obtained from irradiation doses within the range of 125 to 250 Gray. Necrotic embryos were higher than normal embryos when the irradiation doses higher than 250 Gray. The haploid plants were produced mostly from point and arrow-tip embryos. The frequencies of haploid plantlets were 5. 08% and 7. 40% for genotype 330 and 331, respectively. EST-SSR, chloroplast number per guard cell counting and flow cytometry were used to determine the haploid plants in this study. The haploid plants achieved low growth ability and small leaves with sterile stamen.

  14. Oil Well Top Hole Locations, State-oil-08-08, Published in 2008, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Oil Well Top Hole Locations dataset, was produced all or in part from Other information as of 2008. It is described as 'State-oil-08-08'. Data by this publisher...

  15. Oil Well Top Hole Locations, wells-08-2008, Published in 2008, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Oil Well Top Hole Locations dataset, was produced all or in part from Other information as of 2008. It is described as 'wells-08-2008'. Data by this publisher...

  16. Oil Well Top Hole Locations, Oil-May-2006, Published in 2007, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Oil Well Top Hole Locations dataset, was produced all or in part from Other information as of 2007. It is described as 'Oil-May-2006'. Data by this publisher...

  17. Oil Well Top Hole Locations, wells-4-2008, Published in 2008, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Oil Well Top Hole Locations dataset, was produced all or in part from Other information as of 2008. It is described as 'wells-4-2008'. Data by this publisher...

  18. Fire and EMS Districts, Fruitland140, Published in 2001, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Fire and EMS Districts dataset, was produced all or in part from Other information as of 2001. It is described as 'Fruitland140'. Data by this publisher are...

  19. Parcels and Land Ownership, 1s8w-36, Published in 2002, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset, was produced all or in part from Other information as of 2002. It is described as '1s8w-36'. Data by this publisher are...

  20. Plant Distribution and Habitat, Other, Weed Points, Published in 2006, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Plant Distribution and Habitat, Other dataset, was produced all or in part from Other information as of 2006. It is described as 'Weed Points'. Data by this...

  1. Trails, RS2477, TransplanGood, Published in 2007, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Trails, RS2477 dataset, was produced all or in part from Other information as of 2007. It is described as 'TransplanGood'. Data by this publisher are often...

  2. Trails, RS2477, picpts-Project, Published in 2006, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Trails, RS2477 dataset, was produced all or in part from Other information as of 2006. It is described as 'picpts-Project'. Data by this publisher are often...

  3. Trails, RS2477, B-Roads83, Published in 2006, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Trails, RS2477 dataset, was produced all or in part from Other information as of 2006. It is described as 'B-Roads83'. Data by this publisher are often provided...

  4. Trails, RS2477, B-Roads83, Published in 2008, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Trails, RS2477 dataset, was produced all or in part from Other information as of 2008. It is described as 'B-Roads83'. Data by this publisher are often provided...

  5. Parcels and Land Ownership, 1s3w-merged, Published in 2001, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset, was produced all or in part from Other information as of 2001. It is described as '1s3w-merged'. Data by this publisher are...

  6. Parcels and Land Ownership, 1s7w-31, Published in 2002, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset, was produced all or in part from Other information as of 2002. It is described as '1s7w-31'. Data by this publisher are...

  7. Cities, Towns and Villages, cities83, Published in 2008, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Cities, Towns and Villages dataset, was produced all or in part from Other information as of 2008. It is described as 'cities83'. Data by this publisher are...

  8. Parcels and Land Ownership, 1n4w-indian, Published in 2002, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset, was produced all or in part from Other information as of 2002. It is described as '1n4w-indian'. Data by this publisher are...

  9. Parcels and Land Ownership, 1s9w-24, Published in 2002, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset, was produced all or in part from Other information as of 2002. It is described as '1s9w-24'. Data by this publisher are...

  10. Parcels and Land Ownership, 1n2w-36, Published in 2002, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset as of 2002. It is described as '1n2w-36'. Data by this publisher are often provided in UTM coordinate system; in a Transverse...

  11. Cities, Towns and Villages, Bridgeland694, Published in 2002, Duchesne County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Cities, Towns and Villages dataset, was produced all or in part from Other information as of 2002. It is described as 'Bridgeland694'. Data by this publisher...

  12. 镧对西葫芦幼苗模拟酸雨胁迫的缓解效应%Alleviative effects of LaCl_3 on simulated acid rain stresses for Cucurbita pepo seedlings

    Institute of Scientific and Technical Information of China (English)

    边才苗; 王锦文

    2011-01-01

    Using Cucurbita pepo as the experimental material,the alleviative effects of foliar surface spraying for LaCl3(12 mg/L) on seedling growth and three physiological indexes of C.pepo were investigated under some simulated acid rain(SAR) stresses.Results show that the damage effects of SAR on C.pepo seedlings are alleviated by the LaCl3 treatment.The fresh weights of the seedlings treated with LaCl3 solutions of pH 3.5 and pH 3.0 are significantly higher than those of the treatments only with SAR,and are equivalent to 94.7% and 78.6% of the control level(pH 6.5).However,no significant differences are observed between the treatment with other SAR+LaCl3 solution and the treatment only with SAR.The chlorophyll and dissolved protein contents follow the trend of the seedling fresh weight,and the indexes treated with pH 3.0 and pH 3.5 SAR+LaCl3 solutions are higher significantly than that treated only with SAR,but the differences of pH 3.0 are slightly greater than those of pH 3.5.Compared with the control,the dissolved protein content treated with pH 3.5+LaCl3 solution is the highest.The proline content increase consistently with the pH value,and improved by the LaCl3 treatment.The proline contents treated with pH 3.5 and pH 3.0 SAR+LaCl3 solution are higher significantly than that treated only with SAR.Therefore,the alleviative effects of LaCl3 on middle SAR stresses for C.pepo seedling are significant.%以西葫芦为材料,研究模拟酸雨胁迫下适量镧(La)对幼苗生长及叶绿素、可溶性蛋白和脯氨酸含量的影响。结果显示,镧处理对酸雨胁迫有一定的缓解作用。在pH 3.5和3.0时,幼苗鲜重均显著高于模拟酸雨组,并恢复到对照的94.7%和78.6%;但在其他胁迫强度下,镧处理的缓解效应不显著。叶绿素和可溶性蛋白的含量变化与幼苗鲜重类似,在pH 3.0时镧处理的缓解效应最显著;pH 3.5时镧处理的可溶性蛋白含量超过对照。脯氨酸含量呈现持

  13. Cloning and Expression Analysis of PAL Gene in Seed Coat of Cucurbita pepo%美洲南瓜(Cucurbitapepo)种皮苯丙氨酸解氨酶基因克隆与表达分析

    Institute of Scientific and Technical Information of China (English)

    刘佳; 徐秉良; 薛应钰; 张树武; 陈荣贤

    2014-01-01

    Objective]The aim of this study was to clone full-length cDNA of phenylalanine ammonia-lyase (PAL) gene (CP-PAL) in seed coat of the hulled Cucurbita pepo, analyze its expressions in seed coat development of the hulled and hull-less C. pepo, thus providing a theoretical support for revealing the mechanism of seed development and function of accumulation of lignin in seed coat development.[Method]The full sequence of CP-PAL was cloned by RT-PCR and RACE techniques. The bioinformatics method was used to analyze cDNA sequence and deduced amino acid sequence, and the real-time PCR and 2-△△Ct method were used to analyze the expression profile of PAL gene in the whole period of seed coat development. [Result] The full-length sequence of CP-PAL consists of 1 720 bp with an intact open reading frame of 1 359 bp, 5′UTR of 114 bp, 3′UTR of 236 bp, polyA of 11 bp, encoding a polypeptide of 452 amino acids. The molecular weight of CP-PAL was 48.86 kD, pI was 6.5, total number of atoms was 6 885 and the formula was C2158H3449N607O657S14. Homology analysis showed that the CP-PAL nucleotide sequences and deduced amino acids were highly homologous to that of PAL gene in Cucumis sativus. CP-PAL contained three functional domains of PAL-HAL, PLN02457, phe_am_lyase and the typical PAL enzyme active site sequence (GTITASGDLVPLSYIA), and it was one member of Lyase_I_Like superfamily. CP-PAL was most likely to be located in the cytoplasm and endoplasmic reticulum, without signal peptide and leader peptide. And it was non-transmembrane and soluble protein. Besides, CP-PAL included casein kinase II phosphorylation site for four, protein kinase C phosphorylation site for six, N-myristoylation site for twelve and N-glycosylation site for two. In addition, CP-PAL had eighteen serine phosphorylation sites, six threonine phosphorylation sites and five tyrosine phosphorylation sites. Random coil was the maximum structural part in the protein secondary structure of CP-PAL, alpha helix

  14. Fitoextracción en suelos contaminados con cadmio y zinc usando especies vegetales comestibles

    OpenAIRE

    Beltrán Villavicencio, Margarita

    2003-01-01

    En este trabajo se presenta un estudio de fitoextracción en suelos contaminados con cadmio y cadmio-zinc a través de especies vegetales comestibles como la lechuga (Lactuga sativa) en dos variedades, la de hoja rizada (Lactuca sativa crispa) y la romana (Lactuca sativa longifolia), el calabacín (Cucurbita moschata) y el girasol (Helianthus annus). El objetivo fue evaluar la capacidad de asimilación y concentración de cadmio y zinc por las especies vegetales mencionadas como un proceso de rest...

  15. 中国南瓜种质资源信息分析及特征评价

    Institute of Scientific and Technical Information of China (English)

    刘庞源

    2006-01-01

    @@ 一、中国南瓜资源的保存现状 中国南瓜属葫芦科中国南瓜属,学名Cucurbita moschata Duch.,英文名Pumpkin,中国南瓜的果和种子均可食用.中国南瓜的野生祖先原产于墨西哥、北美、危地马拉一带,后传播到欧亚大陆,并在西欧到东亚的广大地区种植.

  16. 保健食品新星——南瓜系列产品

    Institute of Scientific and Technical Information of China (English)

    吴丽玢

    2000-01-01

    @@ 1 前言 南瓜(Cucurbita Moschata Duch)又名倭瓜,为葫芦科南瓜属植物,一年生蔓生藤本.其果肉含瓜氨酸、精氨酸、天门冬素、葫芦巴碱(Trigonelline)、腺嘌呤、胡萝卜素、维生素B、抗坏血酸、脂肪、葡萄糖、蔗糖、戊聚糖及甘露醇等.

  17. Efecto de la endocría en caracteres relacionados con la calidad del fruto de zapallo

    Directory of Open Access Journals (Sweden)

    Baena García Diosdado

    2009-09-01

    Full Text Available Se evaluó el rendimiento y características relacionados con la calidad del fruto de seis introducciones de zapallo, Cucurbita moschata Duch., a libre polinización (S0 y en las líneas endocriadas S1 y S2. La endocría acompañada de selección no causó efectos negativos en las variables producción por planta, espesor de la pulpa del fruto, color de la pulpa del fruto y materia seca en el fruto.

  18. Hypolipidemic effect of the polysaccharides extracted from pumpkin by cellulase-assisted method on mice.

    Science.gov (United States)

    Zhao, Xin-Hua; Qian, Li; Yin, De-Lu; Zhou, Yi

    2014-03-01

    The fruit of pumpkin (Cucurbita moschata) is one of the most important vegetables in the world. This study was conducted to investigate the hypolipidemic effect of the polysaccharide isolated from pumpkin (PP). Male Sprague-Dawley rats were divided into three groups and treated with diets containing either high fat, PP, or normal fat. Oral administration of PP could significantly decrease the levels of plasma triacylglycerol (TG), total cholesterol (TC), and plasma low-density lipoprotein cholesterol and increase the levels of fecal fat, cholesterol, and plasma high-density lipoprotein cholesterol. Therefore, results suggest that PP had a high hypolipidemic activity and could be explored as a possible agent for hyperlipidemia.

  19. The effects of endogenous mycorrhiza (Glomus spp. on plant growth and yield of grafted cucumber (Cucumis sativum l under common commercial greenhouse conditions

    Directory of Open Access Journals (Sweden)

    ISMET BABAJ

    2014-07-01

    Full Text Available The objective of the study was to evaluate the effect of the Arbuscular mycorrhizae (AM fungi on plant growth and yield of grafted cucumber seedlings. The experiment was conducted in a plastic greenhouse located in Shipol, Mitrovica municipality of Kosovo.Graded seeds of cucumber (cv. Ekron F1, and graded seeds of a rootstock (cv. Nimbus F1; C. maxima Duchesne x C. moschata Duchesne, were sown in polysterol trays. Three types of grafted seedlings; self-grafted (SEG, splice grafted (SG and root pruned splice grafted (RPSG were simultaneously produced in equal number as inoculated and non- inoculated with endogenous mycorrhiza. The combined effects of grafting methods and endogenous mycorrhiza (Glomus spp. application on the growth parameters during the nursery period as well as growth rate and yield after transplanting were tested under common commercial conditions. The different grafting methods of cucumber seedlings on C. maxima x C. moschata (SG versus RPSG have significant effects on seedlings growth parameters. Despite of commonly higher relative growth rate of RPSG seedlings till the transplanting time, SG seedlings have a significantly higher total plant dry weight (W. No difference was found regarding leaf dry weight (LW, while a significantly larger leaf area was found for RPSG seedlings. No effect of AM fungi presence was found regarding the growth parameters of grafted cucumber seedlings during the nursery stage, but the presence of AM fungi has significantly improved the growth rate of each grafting method after transplanting, as also increased the total harvested yield. The highest yield was recorded by AM inoculated RPSG seedlings.

  20. Angolan vegetable crops have unique genotypes of potential value for future breeding programmes

    Directory of Open Access Journals (Sweden)

    José P. Domingos

    2016-03-01

    Full Text Available A survey was carried out in Angola with the aim of collecting vegetable crops. Collecting expeditions were conducted in Kwanza-Sul, Benguela, Huíla and Namibe Provinces and a total of 80 accessions belonging to 22 species was collected from farmers and local markets. Species belonging to the Solanaceae (37 accessions and Cucurbitaceae (36 accessions families were the most frequently found with pepper and eggplant being the predominant solanaceous crops collected. Peppers were sold in local markets as a mixture of different types, even different species: Capsicum chinense, C. baccatum, C. frutescens and C. pubescens. Most of the eggplant accessions collected belonged to Solanum aethiopicum L. Gilo Group, the so-called ‘scarlet eggplant’. Cucurbita genus was better represented than the other cucurbit crops. A high morphological variation was present in the Cucurbita maxima and C. moschata accessions. A set of 22 Cucurbita accessions from Angola, along with 32 Cucurbita controls from a wide range of origins, was cultivated in Valencia, Spain and characterised based on morphology and molecularity using a set of 15 microsatellite markers. A strong dependence on latitude was found in most of the accessions and as a result, many accessions did not set fruit. The molecular analysis showed high molecular variability and uniqueness in the collected accessions, as shown by their segregation from the set of global controls. In summary, the material collected is quite valuable because of its uniqueness and the potential of the breeding characteristics it possesses.

  1. Sequence variations in the FAD2 gene in seeded pumpkins.

    Science.gov (United States)

    Ge, Y; Chang, Y; Xu, W L; Cui, C S; Qu, S P

    2015-12-21

    Seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. The fatty acid desaturase-2 (FAD2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. However, little is known about sequence variations in FAD2 in seeded pumpkins. Twenty-seven FAD2 clones from 27 accessions of Cucurbita moschata, Cucurbita maxima, Cucurbita pepo, and Cucurbita ficifolia were obtained (totally 1152 bp; a single gene without introns). More than 90% nucleotide identities were detected among the 27 FAD2 clones. Nucleotide substitution, rather than nucleotide insertion and deletion, led to sequence polymorphism in the 27 FAD2 clones. Furthermore, the 27 FAD2 selected clones all encoded the FAD2 enzyme (delta-12 desaturase) with amino acid sequence identities from 91.7 to 100% for 384 amino acids. The same main-function domain between 47 and 329 amino acids was identified. The four species clustered separately based on differences in the sequences that were identified using the unweighted pair group method with arithmetic mean. Geographic origin and species were found to be closely related to sequence variation in FAD2.

  2. Carotenoid accumulation and carotenogenic gene expression during fruit development in novel interspecific inbred squash lines and their parents.

    Science.gov (United States)

    Nakkanong, Korakot; Yang, Jing Hua; Zhang, Ming Fang

    2012-06-13

    Carotenoid levels and composition during squash fruit development were compared in Cucurbita moschata , Cucurbita maxima , and two lines of their interspecific inbred lines, namely, Maxchata1 and Maxchata2. Eight genes associated with carotenoid biosynthesis were analyzed by quantitative RT-PCR. The two squash species and their interspecific inbred lines exhibited different qualitative and quantitative carotenoid profiles and regulatory mechanisms. C. moschata had the lowest total carotenoid content and mainly accumulated α-carotene and β-carotene, as expected in a fruit with pale-orange flesh. Low carotenoid content in this species was probably due to the comparatively low expression of all genes investigated, especially PSY1 gene, compared to the other squashes. The predominant carotenoids in C. maxima were violaxanthin and lutein, which produced a corresponding yellow flesh color in mature fruit. The relationship between the expression of the CHYB and ZEP genes may result in almost equal concentrations of violaxanthin and lutein in C. maxima at fruit ripening. In contrast, their interspecific inbred lines principally accumulated lutein and β-carotene, leading to orange flesh color. The PSY1 gene exhibited higher expression levels at earlier stages of fruit development in the Maxchata lines, potentially triggering the increased carotenoid accumulation seen in these fruits. Likewise, the higher transcription level of CHYB gene observed in the two interspecific inbred lines might be correlated with high lutein in these hybrids. However, this study could not explain the observed β-carotene accumulation on the basis of gene expression.

  3. Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica

    Directory of Open Access Journals (Sweden)

    Ruth M. Castro

    2013-09-01

    Full Text Available Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ∼1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ∼580 bp corresponding to the coat protein (AV1 was obtained from a chayote (S. edule leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV and Pepper golden mosaic virus (PepGMV were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV infecting chayote in the Western Hemisphere.

  4. Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica.

    Science.gov (United States)

    Castro, Ruth M; Moreira, Lisela; Rojas, María R; Gilbertson, Robert L; Hernández, Eduardo; Mora, Floribeth; Ramírez, Pilar

    2013-09-01

    Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ∼1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ∼580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere.

  5. Efecto de la endocría en caracteres relacionados con la calidad del fruto de zapallo Effect of inbreeding on the quality traits of squash fruit

    Directory of Open Access Journals (Sweden)

    Sanín Ortiz Grisales

    2009-07-01

    Full Text Available Se evaluó el rendimiento y características relacionados con la calidad del fruto de seis introducciones de zapallo, Cucurbita moschata Duch., a libre polinización (S0 y en las líneas endocriadas S1 y S2. La endocría acompañada de selección no causó efectos negativos en las variables producción por planta, espesor de la pulpa del fruto, color de la pulpa del fruto y materia seca en el fruto.Yield and related traits with fruit quality for six squash introductions Cucurbita moschata Duch. from open pollination (S0 and their respective inbreeding lines (S>1 and S2 was measured. It was determined that inbreed accompanied by selection didn't cause negative effects of depression in yield and agribusiness characteristic: plant production, wall fruit thickness , color of the fruit flesh and dry matter in fruit.

  6. 南瓜种质资源遗传多态性的ISSR分析%Analysis of Genetic Diversity in Squash by ISSR Markers

    Institute of Scientific and Technical Information of China (English)

    朱海生; 卢丽芳; 陈敏氡; 林珲; 王彬; 温庆放; 林义章

    2015-01-01

    本研究应用ISSR分子标记技术对85份南瓜种质资源进行遗传多样性分析。结果显示:从100条ISSR引物中筛选出12条引物进行PCR扩增,共获得条带125条,多态性占115条,10条条带为所有南瓜品种所共有,平均每个引物的条带数为10.42条,多态性比例为92%。通过Ntsys2.10e软件计算出85份南瓜资源的遗传相似系数0.3840~1.0000之间。通过聚类分析将所收集的南瓜品种分为三类:分别是美洲南瓜、中国南瓜和印度南瓜,其中亲缘关系较近的是中国南瓜和美洲南瓜,中国南瓜和美洲南瓜与印度南瓜的亲缘关系均比较远。本研究为南瓜种质资源的保护、利用及新品种的培育提供了科学依据。%In this study, the genetic diversity of 85 accessions in Squash was revealed by ISSR markers. 12 polymorphic primers were screened out of 100 polymorphic primers, which generated 125 DNA bands with average 10.42 bands each primer. 115 bands were polymorphic, 10 bands were shared with all tested primers. The ratio of polymorphic bands was 92%, and the genetic similarity coefficient of 85 accessions in squash ranged between 0.384 0 to 1.000 0, which was calculated by Ntsys2.10e. The cluster analysis showed that squashes could be divided into three groups: Cucurbita pepo L., Cucurbita moschata, and Cucurbita maxima. The relationship of Cucurbita moschata and Cucurbita pepo L. was close, whereas their kinship with the Cucurbita maxima was far. This study provided scientific basis for the protection and utilization of squash germplasm resources and cultivation of new varieties.

  7. Ajuste de metodologia da eletroforese de proteínas para obtenção de fenótipos isoenzimáticos de moranga Adjustment of the methodology of protein electrophoresis to obtain isozymatic phenotypes of pumpkin

    Directory of Open Access Journals (Sweden)

    Antonio Teixeira do Amaral Júnior

    1994-01-01

    Full Text Available Modificações no procedimento eletroforético foram introduzidas para uma satisfatória obtenção de fenótipos isoenzimáticos de moranga (Cucurbita maxima Duchesne. Extratos de folhas definitivas de oito acessos de moranga foram submetidos à análise eletroforética para revelação dos seguintes sistemas: esterase (EST; fosfatase ácida (ACP; transaminase do glutamato oxaloacetato (GOT, desidrogenase do malato (MDH e peroxidase (PO. Os resultados apontaram a ineficiência do tampão extrator utilizado e satisfatória qualidade do gel de amido de milho nas resoluções dos sistemas EST, ACP, GOT e MDH. Para o sistema PO, adequado efeito de peneiramento molecular só foi possível com a utilização do amido hidrolisado de batata (Sigma. Ajustes no tempo de cozimento do gel, manuseio da corrente elétrica, preparo de soluções corantes e tempo de revelação indicaram os melhores protocolos ao pro-cedimento eletroforético.Changes in the electrophoresis procedures were introduced to obtain a better isozymatic phenotypes of pumpkin (Cucurbita maxima Duchesne. Extracts of eight access of pumpkin's leaves were subjected to electrophoresis analysis in order to reveal the following systems: esterase (EST, acid phosphatase (ACP, glutamate oxaloacetate transaminase (GOT, malato dehydrogenase (MDH and peroxidase (PO. The results pointed out the inefficiency of the extractor buffer used, and the satisfactory quality of maize starch gel electrophoresis in the resolution of EST, ACP, GOT and MDH systems. For PO system a better effect of "molecular sieving" was only possible when potato starch gel (Sigma was used. Adjusting the starch cooking time, handling the electric current, preparing the staining solutions and revealing time indicated the best protocol to the electrophoresis process.

  8. Breeding of Interspecific Hybrid Squash Rootstock Jingxinzhen No.3 for Melon and Watermelon%甜瓜、西瓜专用砧木品种——京欣砧3号的选育和推广

    Institute of Scientific and Technical Information of China (English)

    贾长才; 李海真; 张帆; 姜立纲

    2011-01-01

    Jingxinzhen No. 3 is interspecific hybrid squash rootstock for melon and watermelon. It was developed by using line 05-88 (Cucwbita maxima) as female parent and line 04-36 (Cucurbita moschata) male parent. Compared with check varieties , Jingxinzhen No. 3 has higher germination, uniform seedling, strong plant vigor. It has high grafting affinity with watermelon and melon scion. The grafted plants are tolerant to low temperature and weak light,resistant to Fusarium wilt,and tolerant to salinity. The grafted plant produces higher yield and good quality fruit.%京欣砧3号是用印度南瓜种05-88自交系作母本,中国南瓜系04-36自交系作父本配制而成的远缘南瓜(Cucurbita maxima×Cucurbita moschata)杂交种,主要用于甜瓜和西瓜嫁接栽培.采用京欣砧3号等不同砧木嫁接甜瓜和西瓜试验结果显示:与其他参试葫芦砧木和各类南瓜砧木相比,京欣砧3号发芽率高,出苗整齐,苗壮.与甜瓜、西瓜嫁接亲和性好,共生力强,结合面致密,成活率高.嫁接苗耐低温弱光.抗枯萎病,耐盐碱,叶部病害轻.嫁接后增产效果明显,对果实品质影响小.

  9. Microscale extraction method for HPLC carotenoid analysis in vegetable matrices

    Directory of Open Access Journals (Sweden)

    Sidney Pacheco

    2014-10-01

    Full Text Available In order to generate simple, efficient analytical methods that are also fast, clean, and economical, and are capable of producing reliable results for a large number of samples, a micro scale extraction method for analysis of carotenoids in vegetable matrices was developed. The efficiency of this adapted method was checked by comparing the results obtained from vegetable matrices, based on extraction equivalence, time required and reagents. Six matrices were used: tomato (Solanum lycopersicum L., carrot (Daucus carota L., sweet potato with orange pulp (Ipomoea batatas (L. Lam., pumpkin (Cucurbita moschata Duch., watermelon (Citrullus lanatus (Thunb. Matsum. & Nakai and sweet potato (Ipomoea batatas (L. Lam. flour. Quantification of the total carotenoids was made by spectrophotometry. Quantification and determination of carotenoid profiles were formulated by High Performance Liquid Chromatography with photodiode array detection. Microscale extraction was faster, cheaper and cleaner than the commonly used one, and advantageous for analytical laboratories.

  10. Cultivar Groups of Cultivated Squash%栽培种南瓜的品种群研究

    Institute of Scientific and Technical Information of China (English)

    林德佩; 刘小俊

    2011-01-01

    作者建议在葫芦科专家Whitaker划分的品种群(cultivar groups)分类基础上,按照果实形状及果形指数为主要指标,结合其他亲缘关系,将3个栽培种南瓜Cuc urb ita moc hata,Cpepo,Cmaxima划分成18个品种群,其中2个(C.pepo的观赏瓜品种群,C.maxima的板栗瓜品种群)是作者新划分的.%Based on Dr. Whitaker's taxonomy for 3 cultivated squash, 18 cultivar groups were suggested for Cucurbita moschata,C. Pepo and C. Maxima using mature fruit shape and fruit shape index as major traits for grouping. Two cultivar groups (the ornamental group for C. Pepo and the panli group for C. Maxima) are newly proposed groups.

  11. Antioxidant capacity and phenolic content of some Thai culinary plants

    Directory of Open Access Journals (Sweden)

    Wiwat Wangcharoen

    2007-08-01

    Full Text Available The antioxidant capacity of red and green bird chili (Capsicum frutescens Linn., red and green chili spur pepper (Capsicum annuum Linn. var. acuminatum Fingerh., red and white holy basil (Ocimum sanctum Linn., garlic (Allium sativum Linn., and pumpkin (Cucurbita moschata Decne., which have been normally used in Thailand as food ingredients, was estimated by three different methods: ferric reducing antioxidant power (FRAP assay, improved ABTS radical cation decolorization assay, and DPPH free radical scavenging assay. Additionally, their total phenolic contents were analyzed by Folin-Ciocalteau micro method. The result showed that red holy basil and red bird chili seemed to be better sources of antioxidant compounds, followed by green bird chili and red chili spur pepper, white holy basil, green chili spur pepper, garlic and pumpkin, respectively. For results of total phenolic content analysis, they were significantly related to those of FRAP (r = 0.825, ABTS (r = 0.973 and DPPH (r = 0.683.

  12. 华青南瓜

    Institute of Scientific and Technical Information of China (English)

    秦玉红; 张宏斌; 位绍文; 刘绍渚

    2004-01-01

    华青南瓜是山东省青岛市农业科学院2000年育成的优良早熟中国南瓜(Cucurbita moschata D.)一代杂种。其母本是从当地农家品种中选育成的自交系,父本是从日本引进的品种古里中选育成的自交系。配制组合后在露地和塑料大棚中进行了3a(年)4个茬次的栽培试验,均表现性状稳定,既适于春、秋露地栽培,也适于秋季日光温室栽培。

  13. Metabolites of 2,4,4'-tribrominated diphenyl ether (BDE-28) in pumpkin after in vivo and in vitro exposure.

    Science.gov (United States)

    Yu, Miao; Liu, Jiyan; Wang, Thanh; Sun, Jianteng; Liu, Runzeng; Jiang, Guibin

    2013-01-01

    There is currently limited knowledge on PBDE metabolism in plants although they could play an important role in the environmental transformation of these persistent organic pollutants. In this study, pumpkin (Cucurbita maxima × C. moschata) was chosen as the model to understand the fate of BDE-28 in plants. MeO-tri-BDEs, OH-tri-BDEs, and OH-tri-BDEs were found as metabolites in plant samples of both in vivo hydroponic and in vitro tissue culture exposure. Three MeO-tri-BDEs were further identified as para-substituted metabolites. MeO-BDEs and OH-BDEs, respectively, accounted for about 1.6% and 1.5% (recovery corrected) of initial amount of BDE-28 according to the semiquantitative results. Other PBDEs, especially less brominated PBDEs as impurities in the standard of BDE-28, were also detected. The impurities and evaporation of the standard must be considered when trace metabolites are studied in exposure experiments.

  14. 西瓜专用砧木——京欣砧4号的选育%Development of a New Watermelon Stock Cultivar, Jingxinzhen No.4 Hybrid

    Institute of Scientific and Technical Information of China (English)

    李海真; 宫国义; 张帆; 贾长才; 姜立纲

    2009-01-01

    京欣砧4号是用母本04-63和父本04-217配制而成的南瓜(Cucurbita moschata)杂交种,是西瓜专用优良砧木,其发芽率高,出苗齐整、苗壮;与西瓜嫁接亲和性好,共生亲和力强,结合面致密;嫁接苗在低温弱光下生长强健,根系发达,吸肥力强,成活率高;嫁接瓜果实大、产量高,嫁接后对果实的品质影响小.高抗枯萎病,叶部病害轻.

  15. 迷你南瓜银叶病的发生与防治%Occurrence and Control of Squash Silverleaf Disease

    Institute of Scientific and Technical Information of China (English)

    陈军; 陈国元; 林惠鸣

    2013-01-01

    迷你南瓜(Cucurbita moschata)银叶病典型症状表现为叶面银灰色,主要由银叶粉虱(Bemisia argentifolii Bellows&Perring)传播,发病程度因受光照、温度、湿度的影响而有所变化,据此提出迷你南瓜银叶病的有效防治措施.%Typical symptoms of squash silverleaf disease was silver-gray foliage.The disease was mainly transmited by the silverleaf whitefly (Bemisia argentifolii Bellows & Perring).The incidence is affected by light,temperature,humidity change.Accordingly,effective prevention and control measures about squash silverleaf disease were proposed.

  16. 食用菌深层发酵法制备南瓜汁脱糖营养液工艺研究%The Making of Nutritious Beverage Glucose Removed from Pumpkin Juice by Edible Fungi Submerged Fermentation

    Institute of Scientific and Technical Information of China (English)

    刘志强; 傅承新; 杨志坚; 林小青; 吕月珍

    2003-01-01

    本项实验研究以南瓜(Cucurbita moschata Duch)汁为原料,经食用菌深层发发酵脱糖获得营养液的制备工艺,适宜条件为:发酵液为黄狼南瓜汁,果泥含量20%、灵芝(Ganoderma lueidum)L203PDA液体种,接种量1:10,通汽量1:1.5,发酵温度26±1℃.发酵72h后,经过滤制取脱糖营养液.产品中含有多种氨基酸,并对四氧嘧啶糖尿病型小鼠有降血糖和预防血糖升高的营养效果.

  17. 南瓜多糖降血糖有效部位的提取分离及检测%Extraction,separation, and examination of the effective hypoglycemic component of cushaw polysaccharide

    Institute of Scientific and Technical Information of China (English)

    熊学敏; 石扬; 康明; 曹珏; 赵银鹰

    2001-01-01

    目的:对具有降血糖作用的南瓜多糖粗品进行提取、分离和纯度检测.方法:将葫芦科植物南瓜Cucurbita moschata(Duch.)Poiret用水提、醇沉后经大孔离子柱和Sepharose柱、Sephadex A-200柱分离,获得多糖粗品,冷冻干燥后得白色粉末多糖,对该南瓜多糖有效组分经纸层析分析和玻璃纤维纸电泳纯度检查.结果:纯度检查不多于3个紫红色斑点.结论:该南瓜多糖有效部位为相对单一组分.

  18. Genes for resistance to zucchini yellow mosaic in tropical pumpkin.

    Science.gov (United States)

    Pachner, Martin; Paris, Harry S; Lelley, Tamas

    2011-01-01

    Four cultigens of Cucurbita moschata resistant to zucchini yellow mosaic virus were crossed with the susceptible 'Waltham Butternut' and with each other in order to clarify the mode of inheritance of resistance and relationships among the genes involved. Five loci were segregating, with genes for resistance Zym-0 and Zym-4 carried by 'Nigerian Local' and one of them also carried by 'Nicklow's Delight,' Zym-1 carried by 'Menina,' and zym-6 carried by 'Soler.' A recessive gene carried by 'Waltham Butternut,' zym-5, is complementary with the dominant Zym-4 of 'Nigerian Local,' that is, the resistance conferred by Zym-4 is only expressed in zym-5/zym-5 individuals. Gene zym-6 appears to be linked to either Zym-0 or Zym-4, and it is also possible that Zym-1 is linked to one of them as well.

  19. LIKUIFASI ENZIMATIK β - KAROTEN SEBAGAI FUNCTIONAL FOOD YANG TERDAPAT DALAM POMACE DARI BUAH LABU KUNING

    Directory of Open Access Journals (Sweden)

    Santosa Herry

    2012-07-01

    Full Text Available Attempts to separate the β carotene contained in pomace or pulp byproduct pumpkin fruit juicing process(Cucurbitae moschata is potential. The magnitude of benefit of β-carotene as a chemo preventive, high contentof β-carotene in pumpkin, strong pumpkin fruit shelf life and high content of β-carotene in the pomace, an initialconsideration of this research. β-Carotene enzmatik liquefaction using pectinase enzyme combination (P andcellulose (C, subject to the pomace. liquefaction / hydrolysis carried out in a stirred tank reactor to determine:the most influential variable ratio between enzyme ratio P: C; temperature and pH. Influential process variableswas determined using two-level factorial design method Quicker. From the experimental results of the data if itis known that the degree of acidity of pH is the single most influential variable in the process of β-caroteneliquefaction presents in pumpkin fruit pomace.

  20. Effect of different drying methods on moisture ratio and rehydration of pumpkin slices.

    Science.gov (United States)

    Seremet Ceclu, Liliana; Botez, Elisabeta; Nistor, Oana-Viorela; Andronoiu, Doina Georgeta; Mocanu, Gabriel-Danut

    2016-03-15

    This study was carried to determine the influence of hot air drying process and combined methods on physicochemical properties of pumpkin (Cucurbita moschata) samples. The experiments in hot air chamber were lead at 50, 60 and 70 °C. The combined method consists of a triple combination of the main drying techniques. Thus, in first stage the samples were dried in hot air convection at 60 °C followed by hot air ventilation at 40 °C simultaneous with microwave. The time required to reduce the moisture content to any given level was highly dependent on the drying conditions. So, the highest value of drying time in hot air has been 540 min at 50 °C, while the lowest time has been 189 min in hot air combined by microwave at 40 °C and a power of 315 W. The samples dried by hot air shows a higher rehydration capacity than samples dried by combined method.

  1. Pharmacokinetics of doxycycline after a single intravenous, oral or intramuscular dose in Muscovy ducks (Cairina moschata).

    Science.gov (United States)

    Yang, F; Sun, N; Zhao, Z S; Wang, G Y; Wang, M F

    2015-01-01

    1. The pharmacokinetics of doxycycline in ducks were investigated after a single intravenous (IV), intramuscular (IM) or oral (PO) dose at 20 mg/kg body weight. 2. The concentrations of doxycycline in plasma samples were assayed using a high performance liquid chromatography method, and pharmacokinetic parameters were calculated using a non-compartmental model. 3. After IV administration, doxycycline had a mean (±SD) distribution volume (Vz) of 1761.9 ± 328.5 ml/kg and was slowly eliminated with a terminal half-life (t₁/₂λz) of 21.21±1.47 h and a total body clearance (Cl) of 57.51 ± 9.50 ml/h/kg. Following PO and IM administration, doxycycline was relatively slowly absorbed - the peak concentrations (Cmax) were 17.57 ± 4.66 μg/ml at 2 h and 25.01 ± 4.18 μg/ml at 1.5 h, respectively. The absolute bioavailabilities (F) of doxycycline after PO and IM administration were 39.13% and 70.71%, respectively. 4. The plasma profile of doxycycline exhibited favourable pharmacokinetics characteristics in Muscovy ducks, such as wide distribution, relatively slow absorption and slow elimination, though oral bioavailability was low.

  2. Increase in Zucchini yellow mosaic virus Symptom Severity in Tolerant Zucchini Cultivars Is Related to a Point Mutation in P3 Protein and Is Associated with a Loss of Relative Fitness on Susceptible Plants.

    Science.gov (United States)

    Desbiez, C; Gal-On, A; Girard, M; Wipf-Scheibel, C; Lecoq, H

    2003-12-01

    ABSTRACT Zucchini yellow mosaic virus (ZYMV, Potyvirus) is a very damaging cucurbit virus worldwide. Interspecific crosses with resistant Cucurbita moschata have led to the release of "resistant" zucchini squash (C. pepo) F(1) hybrids. However, although the resistance is almost complete in C. moschata, the commercial C. pepo hybrids are only tolerant. ZYMV evolution toward increased aggressiveness on tolerant hybrids was observed in the field and was obtained experimentally. Sequence comparisons and recombination experiments revealed that a point mutation in the P3 protein of ZYMV was enough to induce tolerance breaking. Competition experiments were performed between quasi-isogenic wild-type, and aggressive variants of ZYMV distinguished by monoclonal antibodies. The aggressive mutants were more fit than wild-type strains in mixed infections of tolerant zucchini, but they presented a drastic fitness loss in mixed infections of susceptible zucchini or melon. Thus, the ability to induce severe symptoms in tolerant zucchini is related to a genetic load in susceptible zucchini, but also on other susceptible hosts. This represents the first quantitative study of the fitness cost associated with tolerance breaking for a plant virus. Thus, although easily broken, the tolerance might prove durable in some conditions if the aggressive variants are counterselected in susceptible crops.

  3. [Potential allelopathic effects of Piper nigrum, Mangifera indica and Clausena lansium].

    Science.gov (United States)

    Yan, Guijun; Zhu, Chaohua; Luo, Yanping; Yang, Ye; Wei, Jinju

    2006-09-01

    With Piper nigrum, Mangifera indica and Clausena lansium as the donators, this paper studied their potential allelopathic effects on the germination and growth of Zea mays, Glycine max, Cucurbita moschata, Arachis hypogaea, Raphanus sativus, Echinochloa crusgalli, Digitaria sanguinalis and Stylosanthes guianensis. The results showed that the aqueous extracts of these donators could inhibit the germination and growth of Z. mays, G. max, C. moschata, E. crus-galli and D. sanguinalis at high concentration, but stimulate them at low concentration. In rhizosphere soil of P. nigrum and M. indica, the germination and growth of Z. mays L was stimulated, while A. hypogaea was inhibited. The aqueous extracts of the donators were extracted by ethyl acetate and n-butanol, respectively, and the inhibitory activity of both aqueous and n-butanol fractions from P. nigrum and M. indica on Z. mays, R. sativus and S. guianensis was stronger than that of ethyl acetate fraction, indicating that P. nigrum and M. indica contained the allelochemicals with high polarity.

  4. Grafting of Romanian Melons and Watermelons for Culture from South Area of Romania

    Directory of Open Access Journals (Sweden)

    Dorin Sora

    2016-11-01

    Full Text Available The vegetable grafting is useful in Romania; it is more difficult in watermelons and melons and it is continuously developing. The research was aimed the establishing of the technological stages for seedling producing of scions (Romanian melons and watermelons and rootstocks (F1 hybrids of Lagenaria siceraria and Cucurbita maxima x C. moschata for obtaining of grafted plant seedlings. The experience was realized out on a collection consisting from two Romanian scions, melon (‘Fondant’ variety and watermelon (‘Dochiţa’ variety obtained at Research and Development Station for Vegetable Growing Buzău and two rootstocks, bottle gourd - L. siceraria (‘Emphasis’ F1 and interspecific hybrid squash - C. maxima x C. moschata (‘Cobalt’ F1. The obtaining of scion and rootstock plants was made according to the ecological requirements of the species. The grafting was made by annexation (splice grafting. The plants had optimal diameters for splice grafting. Between scions (‘Fondant’ and ‘Dochiţa’ are no diference, statistical analysis could not be performed. Technological stages for producing grafted seedlings of Romanian melon and watermelon were established. The grafting was performed successfully for cucurbit symbiotes (scions and rootstocks. These technological stages for grafting by annexation of Romanian melons and watermelons are recommended for cultures in the south area of Romania.

  5. Characterization of the small RNA component of leaves and fruits from four different cucurbit species

    Directory of Open Access Journals (Sweden)

    Jagadeeswaran Guru

    2012-07-01

    Full Text Available Abstract Background MicroRNAs (miRNAs are a class of non-coding small RNAs involved in post-transcriptional regulation of gene expression critical for plant growth and development, stress responses and other diverse biological processes in plants. The Cucurbitaceae or cucurbit family represents some of economically important species, particularly those with edible and medicinal fruits. Genomic tools for the molecular analysis of members of this family are just emerging. Partial draft genome sequence became available recently for cucumber and watermelon facilitating investigation of the small RNA component of the transcriptomes in cucurbits. Results We generated four small RNA libraries from bottle gourd (Lagenaria siceraria, Cucurbita moschata, Cucurbita pepo, and, watermelon (Citrullus lanatus var. lanatus in order to identify conserved and novel lineage specific miRNAs in these cucurbits. Deep sequencing of small RNA libraries from these species resulted in 1,597,263, 532,948, 601,388, and 493,384 unique sRNA reads from bottle gourd, moschata, pepo and watermelon, respectively. Sequence analysis of these four libraries resulted in identification of 21 miRNA families that are highly conserved and 8 miRNA families that are moderately conserved in diverse dicots. We also identified 4 putative novel miRNAs in these plant species. Furthermore, the tasiRNAs were identified and their biogenesis was determined in these cucurbits. Small RNA blot analysis or q-PCR analyses of leaf and fruit tissues of these cucurbits showed differential expression of several conserved miRNAs. Interestingly, the abundance of several miRNAs in leaves and fruits of closely related C. moschata and C. pepo was also distinctly different. Target genes for the most conserved miRNAs are also predicted. Conclusion High-throughput sequencing of small RNA libraries from four cucurbit species has provided a glimpse of small RNA component in their transcriptomes. The analysis also

  6. Effects of NaCl Stress on Cation Contents in Seedlings of Two Pumpkin Varieties%NaCl胁迫对两种南瓜幼苗离子含量的影响

    Institute of Scientific and Technical Information of China (English)

    王冉; 陈贵林; 宋炜; 吕桂云; 梁静; 李卫欣

    2006-01-01

    NaCl胁迫7 d明显抑制了两种南瓜--黑籽南瓜(Cucurbita ficifolia Bouche.)和白籽南瓜(Cucurbita moschata Duch.)幼苗地上部和根系的生长,使地上部相对含水量明显下降.随NaCl胁迫浓度(150、300和500mmol/L)的提高,两种南瓜幼苗根、茎和叶片中Na+含量明显增加,K+含量明显下降.Na+和K+含量在两个南瓜品种各器官中的分布规律分别是:根>茎>叶片和茎>叶片>根.NaCl胁迫后黑籽南瓜根、茎和叶片Na+含量远低于白籽南瓜,而叶片游离脯氨酸含量、可溶性糖含量和地上部相对含水量明显高于白籽南瓜.表明两者在渗透调节方式、离子的吸收和运输能力上有差异,黑籽南瓜耐盐性较强.

  7. 黄瓜专用砧木品种京欣砧6号的选育%A New Cucumber Rootstock Hybrid——Jingxinzhen No.6

    Institute of Scientific and Technical Information of China (English)

    李海真; 张帆; 贾长才; 姜立纲; 张国裕

    2011-01-01

    Jingxinzhen No. 6 is a cucumber hybrid rootstock developed by crossing winter squash (Cucurbita moschata Duch.)lines N11 as female parent and N78 as male parent. It has good compatibility and affinity when cucumber scion is grafted on. The grafted plants are tolerant to the low temperature and low light,and resistant to Fusarium wilt. The grafted cucumber plants produce higher yield and fruits with shiny dark green skin and good quality. It is adapted to spring and winter protected production of cucumber.%京欣砧6号是以自交系N11为母本,N78为父本配制而成的中国南瓜(Cucurbita rrtoschata Duch.)杂交种.该砧木品种嫁接黄瓜亲和力好,共生亲和力强,成活率高,结合面致密,耐低温弱光,抗枯萎病,有促进黄瓜生长、增强其抗病能力和提高黄瓜产量的效果.嫁接黄瓜后,瓜条亮绿无蜡粉,明显提高了黄瓜商品品质.适宜早春和越冬保护地黄瓜嫁接栽培.

  8. [Effects of host plants on the life table parameters of experimental populations of Aphis gossypii].

    Science.gov (United States)

    Li, Yan-Yan; Zhou, Xiao-Rong; Pang, Bao-Ping; Chang, Jing

    2013-05-01

    A comparative study was conducted on the life table parameters of Aphis gossypii reared on five host plant species at (25 +/- 1) degrees C in laboratory. There existed significant differences in the durations of various developmental stages, adult longevity, mean offspring number per day, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time, and population doubling time for the A. gossypii populations reared on the host plants. For the aphids on Lagenaria siceraria var. turbinate, they needed the longest time (5.84 days) to complete one generation, but for those on the other four plants, no significant differences were observed, with the time needed ranged from 5.24 to 5.45 days. The adult longevity was the longest (20.04 days) on Cucumis sativus, but had no significant differences on the other four host plants, being from 14.76 to 16.03 days. The populations' survival curves on all test host plants were of Deevey I, i. e., the death mainly occurred during late period. The survival rate on C. sativus was higher than those on the other four host plants. Based on the intrinsic rates of increase of A. gossypii, its host suitability was in the order of Cucumis melo var. saccharinus > Lagenaria siceraria var. turbinate > Cucurbita moschata var. melonaeformis > Cucumis sativus > Cucurbita pepo var. medullosa.

  9. 某矿区附近不同作物对3种重金属富集能力的研究%Study on the Concentration Capacity to Three Kinds of Heavy Metals for Different Crops Around the Gold Area

    Institute of Scientific and Technical Information of China (English)

    李庚飞

    2012-01-01

    为了研究不同作物对重金属的积累特征,以陕西省某金矿生产区及附近不同区段生长的4种作物——玉米(Zea mays L.)、南瓜(Cucurbita moschata)、野大豆(Glycine javanica L.)和黄豆(Giycine max (L) Merril)为研究对象,采用火焰原子吸收光谱法测定并分析其体内铜、镉和锌含量,以便确定建立人工生态系统的植物种类.结果表明:除野大豆根际的重金属Zn外,4种作物根际土壤中的3种重金属单项污染指数均大于1,均处于污染状态;玉米、黄豆和南瓜对Zn的富集能力均较强,其中以南瓜的富集能力最强,其富集系数为7.11,转移系数为43.18;野大豆和玉米对Cu的富集能力较强;而4种作物对Cd的富集能力均较弱,虽然转移系数大于1,但富集系数均小于1.%In order to study accumulation property of the heavy metals in different crops and establish the plant species of artificial eco-system, the author chose four species crops as subjects, including Zea mays L., Cucurbits moschata, Glycine javanica L., Giycine max (L) Merril, investigated around the golden mineral in Shaanxi Province and determined the concentration of the heavy metals by applying flame atomic absorption spectrometry, including copper, zinc and cadmium. The results showed that, except the rhizospheric soil of Glycine javanica L. was clean to Zn, the rhizospheric soils of four species crops were contaminated by 3 kinds of metals because their single factor pollution index were greater than 1. Among these crops, the concentration capacity to zinc was all powerful for Zea mays L., Giycine max (L) Merril and Cucurbita moschata. Especially Cucurbita moschata had the biggest transfer factors and concentration factors to Zn, and they were 43.18 and 7.11 individually. Glycine javanica L. and Zea mays L. had the powerful concentration capacity to copper. Although all crops transfer factors were more than 1, their concentration factors were all less than 1, so all

  10. Effects of Different Rotation Modes on Microbial Population in Gravel Mulched Field%砂田轮作模式对土壤微生物区系的影响

    Institute of Scientific and Technical Information of China (English)

    赵亚慧; 吴宏亮; 康建宏; 许强; 杨金娟; 姚姗; 尹冠华

    2012-01-01

    [目的]研究砂田轮作模式对土壤微生物区系的影响,从而通过制定合理的栽培制度创造良好的土壤生态环境,为促进农业的可持续发展提供理论依据.[方法]对宁夏中卫市香山地区压砂地土壤进行取样,并进行了微生物区系的分析.[结果]西瓜(Citrullus lanatus)与绿豆(Vigna radiata)、南瓜(Cucurbita moschata)、油葵(Helianthus annuus)、西葫芦(Cucurbita pepo L.)、芝麻(Sesamum indicum)轮作,土壤微生物多样性指数均高于西瓜连作.[结论]轮作能有效调节土壤微生物区系,有利于微生物群落的多样性和稳定性的提高,最终改善了土壤的微生态环境,其中以西瓜→南瓜效果最好.%[ Objective] The purpose was to study the effects of different rotation modes on microbial population in gravel mulched field, then create good soil ecological environment by drawing up reasonable cultivation system to provide a theoretical basis for promoting agricultural sustainable development of agriculture, [Method] Soil samples were taken from gravel mulched field in Xiangshan Area, Zhongwei City, Ningxia Hui Autonomous Region and their microbial population was analyzed. [Result] Compared with the watermelon continuous crop, the microbial diversity index was higher in the planting mode of mung bean( Vigna radiata) , pumpkins( Cucurbita moschata) , oil sunflower(Helianthus arm-uus) , squash(Cucurbitapepo L. ) and sesame(Sesamum indicum) rotating with watermelon( Citrultus lanatus). [Conclusion] Rotating effectively adjusts soil microbial population, which is conducive to improving microbial population diversity and stability, and eventually improving the soil ecology environment. Among these rotation modes, squash rotation with watermelon was the best.

  11. Temperatura e embalagem para abóbora minimamente processada Temperature and packaging of minimally processed pumpkin (Curcubita moschata

    Directory of Open Access Journals (Sweden)

    Ana Veruska Cruzda Silva

    2009-06-01

    Full Text Available O objetivo deste trabalho foi avaliar temperaturas de armazenamento e embalagens para abóbora minimamente processada. Pedaços de abóbora foram cortados em tamanho de 5 x 10 cm, embalados em bandejas de poliestireno recobertas com filme polivinilcloreto e em embalagem de polietileno de alta densidade a vácuo. O produto foi mantido a 5 e 10 °C por um período de 12 dias. A cada três dias avaliou-se o teor de sólidos solúveis, acidez total titulável, pH, vitamina C e coloração. Os resultados mostraram não haver diferenças significativas entre as duas temperaturas de refrigeração utilizadas na conservação da abóbora. Entretanto, a embalagem com filme PVC permitiu maior conservação dos atributos de qualidade da abóbora até o 9º dia, com exceção da cor, que sofreu menores alterações quando usada embalagem a vácuo.The present work aimed to evaluate the efficiency of different storage temperatures and packing materials for pumpkin fresh cuts. Pumpkin cuts of 5 x 10 cm were packed in polystyrene trays covered with polivynilchloride film or in vacuum high density polyethylene bags. The trays and bags were kept at 5 and 10 °C for 12 days. Soluble solids, total titratable acidity, pH, vitamin C, and color of pumpkin cuts were evaluated every 3 days. The different temperatures did not affect the storage of the pumpkins. However, packaging with PVC film allowed a longer conservation by keeping the pumpkin quality attributes up to the 9th day, except for the color which undergone minor alterations when stored within a vacuum pack.

  12. Sexual size dimorphism and sex determination by morphometric measurements in locally adapted muscovy duck (Cairina moschata in Nigeria

    Directory of Open Access Journals (Sweden)

    Abel Olusegun OGUNTUNJI

    2015-11-01

    Full Text Available Sexual size dimorphism (SSD is a common phenomenon in most mammalian and poultry species. This study was undertaken to investigate SSD in nondescript locally adapted Muscovy ducks in Nigeria by applying descriptive analysis, sexual dimorphism index (SDI, sexual size dimorphism index (SSDI and Principal Component analysis to morphological variables. Besides, attempt was made to identify best morphological predictors of sex using Stepwise Discriminant analysis. Data on ten morphological traits; body weight, body girth, body length, wing length, shank length, shank circumference, thigh length, total leg length, bill length and bill width were taken from 1,020 extensively managed adult Muscovy ducks comprising 287 males and 733 females. These birds were randomly sampled from Rain Forest, Derived Savanna and Guinea Savanna agro-ecological zones of Nigeria. Sex significantly (P < 0.001 affected all morphometric measurements in favour of males. Assessment of relative contribution of morphological traits to SSD through SDI and SSDI revealed that body weight followed by skeletal frame-related variables (body length and wing length were the most dimorphic traits. Three Principal Components were generated for each sex; accounting for 62.70 % and 54.24 % of the total variance explained of male and female ducks, respectively. In tandem with the SDI and SSDI results, Stepwise Discriminant analysis indicated body weight as the best predictor of sex of adult Muscovy ducks followed by wing length and body length; correctly classifying 98.5 % of ducks and are reliable for sex determination in the field. The established SSD in this study could be employed for description, utilization, selection and planning of improvement programs of male and female Muscovy ducks.

  13. Sexual size dimorphism and sex determination by morphometric measurements in locally adapted muscovy duck (Cairina moschata) in Nigeria:

    OpenAIRE

    2014-01-01

    Sexual size dimorphism (SSD) is a common phenomenon in most mammalian and poultry species. This study was undertaken to investigate SSD in nondescript locally adapted Muscovy ducks in Nigeria by applying descriptive analysis, sexual dimorphism index (SDI), sexual size dimorphism index (SSDI) and Principal Component analysis to morphological variables. Besides, attempt was made to identify best morphological predictors of sex using Stepwise Discriminant analysis. Data on ten morphological trai...

  14. Thyroid status affects membranes susceptibility to free radicals and oxidative balance in skeletal muscle of Muscovy ducklings (Cairina moschata).

    Science.gov (United States)

    Rey, Benjamin; Romestaing, Caroline; Bodennec, Jacques; Dumet, Adeline; Fongy, Anaïs; Duchamp, Claude; Roussel, Damien

    2014-10-01

    Thyroid hormones (TH) are major contributor to oxidative stress in mammals because they (1) stimulate reactive oxygen species generation (ROS), (2) impair antioxidant defenses, and (3) increase the susceptibility to free radicals of most tissues. Unlike mammals, THs seem to diminish mitochondrial ROS while they have limited effect on the antioxidant machinery in birds. However, how THs modify the susceptibility to ROS has never been explored in an avian model, and very little is known about their effect on oxidative balance in birds. Therefore, the objective of our study was to examine the effect of chronic pharmacological hypo- and hyperthyroidism on (i) the susceptibility of mitochondrial membranes to ROS; and (ii) the level of oxidative stress assessed by measuring oxidative damage to lipids, nucleic acids and proteins in the gastrocnemius muscle of ducklings. We show that hypothyroidism had no effect on the susceptibility of mitochondrial membranes to free radicals. Hypothyroid ducklings had lower oxidized lipids (-31%) and DNA (-25%) but a similar level of protein carbonylation relative to controls. Conversely, mitochondrial membranes of hyperthyroid ducklings exhibited higher unsaturation (+12%) and peroxidation (+31%) indexes than in controls indicating a greater susceptibility to free radicals. However, hyperthyroid ducklings exhibited more oxidative damages on proteins (+67%) only, whereas lipid damages remained unchanged, and there was a slight reduction (-15%) in damages to DNA compared to euthyroid controls. Our results indicate that birds and mammals present fundamental differences in their oxidative stress response to thyroid status.

  15. Phloem long-distance delivery of FLOWERING LOCUS T (FT) to the apex.

    Science.gov (United States)

    Yoo, Soo-Cheul; Chen, Cheng; Rojas, Maria; Daimon, Yasufumi; Ham, Byung-Kook; Araki, Takashi; Lucas, William J

    2013-08-01

    Cucurbita moschata FLOWERING LOCUS T-LIKE 2 (hereafter FTL2) and Arabidopsis thaliana (Arabidopsis) FLOWERING LOCUS T (FT), components of the plant florigenic signaling system, move long-distance through the phloem from source leaves to the vegetative apex where they mediate floral induction. The mechanisms involved in long-distance trafficking of FT/FTL2 remain to be elucidated. In this study, we identified the critical motifs on both FT and FTL2 required for cell-to-cell trafficking through mutant analyses using a zucchini yellow mosaic virus expression vector. Western blot analysis, performed on phloem sap collected from just beneath the vegetative apex of C. moschata plants, established that all mutant proteins tested retained the ability to enter the phloem translocation stream. However, immunolocalization studies revealed that a number of these FTL2/FT mutants were defective in the post-phloem zone, suggesting that a regulation mechanism for FT trafficking exists in the post-phloem unloading step. The selective movements of FT/FTL2 were further observed by microinjection and trichome rescue studies, which revealed that FT/FTL2 has the ability to dilate plasmodesmata microchannels during the process of cell-to-cell trafficking, and various mutants were compromised in their capacity to traffic through plasmodesmata. Based on these findings, a model is presented to account for the mechanism by which FT/FTL2 enters the phloem translocation stream and subsequently exits the phloem and enters the apical tissue, where it initiates the vegetative to floral transition.

  16. Screening of hosts for artificial multiplication of Parasaissetia nigra (Nietner)%人工扩繁橡胶盔蚧的寄主材料的筛选

    Institute of Scientific and Technical Information of China (English)

    王进强; 许丽月; 李国华; 周明

    2013-01-01

    To screen hosts for multiplication of Parasaissetia nigra and its parasitiods wasps indoor, rearing test was done using seedlings of Hevea brasiliensis, seedlings of Solanum tuherosum, seedlings of Hibiscus rosa - sinensis, fruits of Citrus limon and fruits of Cucurbita moschata. In the process of the test, eight terms were compared respectively with hosts including survival rate of the scale insects, degree of difficulty to inoculate the scale insects, fitness of the scale insects to hosts, storage time of the hosts, whether hosts suiting for growing or placement indoor or not, hosts' management level of difficulty, the level of difficulty to inoculate or withdraw parasitic wasps and to prevent their escape. Finally, C. moschata was confirmed as the optimal host.%为筛选出能在室内大量繁殖橡胶盔蚧及其寄生蜂的寄主材料,用橡胶苗、马铃薯苗、扶桑苗、柠檬和南瓜等5种材料进行了饲养试验,通过比较蚧虫存活率、接种蚧虫的难易、蚧虫适宜度、寄主材料的耐保存度、寄主材料室内生长和存放的适合度、寄主材料的管理难易、接种和回收寄生蜂的难易、防止寄生蜂逃逸的难易等8项指标,最终确定南瓜为理想的寄主材料.

  17. Anti-hyperglycaemic globulins from selected Cucurbitaceae seeds used as antidiabetic medicinal plants in Africa

    Science.gov (United States)

    2013-01-01

    Background The recent epidemic of diabetes mellitus (DM) in Africa, coupled with rampant poverty, is an indication of the urgent need to develop new efficacious, cheaper and more available drugs to face this growing public health challenge. A number of plants products among which the protein-rich Cucurbitaceae seeds are commonly used in traditional medicine with increasing acclaimed efficacy against DM. The aim of this study was to analyse and evaluate the hypoglycaemic activity of storage proteins of five species of Cucurbitaceae, which include Telfairia occidentalis, Citrullus lanatus, Lagenaria siceraria, Cucumeropsis mannii and Cucurbita moschata. Methods The different families of storage proteins were extracted following differential solubility, and their contents were estimated using the Bradford method. The analysis of these proteins was done by electrophoresis in non-denaturing and denaturing conditions. The evaluation of hypoglycaemic properties of various globulins extracted was performed on male Wistar rats by the oral glucose tolerance test. Results The results showed that among the proteins extracted, globulins constitute the most abundant class of storage proteins in all five species selected. Citrullus lanatus and Cucurbita moschata presented the highest levels of globulin (275.34 and 295.11 mg/g dry matter, respectively). The results of electrophoresis showed that all species possess acidic and neutrals albumins and globulins, with molecular weight of protein subunits ranging from 6.36-44.11 kDa for albumins, 6.5-173.86 kDa for globulins and 6.5-49.66 kDa for glutelins. The 6.36 kDa of albumin subunit protein and the 6.5 kDa of globulin subunit protein were present in all the species. The oral glucose tolerance test showed that the globulins of the seeds of all species except Cucumeropsis mannii caused significant drop in blood sugar (88 – 137.80%, compared to the controls, p<0.05). Conclusions These findings showed that the selected Cucurbitaceae

  18. Unapal-Llanogrande, nuevo cultivar de zapallo adaptado a las condiciones del valle geográfico del río Cauca, Colombia Unapal Llanogrande, pumkim new cultivar adapted to the Cauca Valley, Colombia

    Directory of Open Access Journals (Sweden)

    Edgar Iván Estrada Salazar

    2010-04-01

    Full Text Available El nuevo cultivar de zapallo (Cucurbita moschata Unapal-Llanogrande se originó a partir de la población básica P-34 obtenida en la segunda colección colombiana de germoplasma realizada por Montes (2003. La población heterogénea (P-34 fue sometida a tres ciclos de selección recurrente fenotípica para los caracteres: producción por planta, prolificidad, tamaño de fruto pequeño (2 - 3 kg, calidad de fruto y sanidad de la planta. El cultivar expresa alta uniformidad y estabilidad fenotípica para hábito de crecimiento postrado con moderado número de guías, tallo cilíndrico angular y pubescente, hojas lobuladas reniformes pequeñas, con alta frecuencia de manchas plateadas, floración monoica, inicio de floración estaminada y pistilada entre 60-70 días después de la emergencia, inicio de cosecha de 130 a140 días, con plantas de alta prolificidad (9 a 11 frutos y alto rendimiento (entre 10 y 20 kg/planta. El fruto es redondo con superficie lisa, sin costillar, de color verde brillante y manchas amarillas o rojizas en la madurez, el peso varía entre 1.5 y 3.0 kg, el diámetro de pulpa entre 3.8 y 4.8 cm y el de cavidad placentaria entre 9 y 12 cm. El color de pulpa es amarillo intenso.Unapal-Llanogrande, is a pumkin (Cucurbita moschata breeding population, originated from the second germoplasm colombian colection obtained for Consuelo Montes (2003. The heterogeneous population P-34 was subjected to three cycles of phenotypic recurrent selection that were carried out at Experimental Center of the National University of Colombia, Palmira, Valle del Cauca. Plant production, plant prolific, small fruit weight, fruit quality and tolerance to diseases and pests were evaluated. The Unapal-Llanogrande, had height uniformity and stability postrate growth habit, low number of branch per plant, 60-70 days to male and female flowering, 130-140 days to harvesting. Fruits had a round shape, green whit yellow an red spot colors at maturity, fruit

  19. Condutividade elétrica em sementes de abóbora, híbrido Bárbara Electrical conductivity of pumpkin hybrid Bárbara seeds

    Directory of Open Access Journals (Sweden)

    Roberval Daiton Vieira

    2006-09-01

    Full Text Available A condutividade elétrica na solução de embebição de sementes tem sido um procedimento usado com sucesso para avaliar o vigor de sementes. A presente pesquisa foi desenvolvida com o objetivo de determinar os procedimentos adequados para o teste de condutividade elétrica de sementes de abóbora (Cucurbita moschata Duch ex. Lam. Foram utilizadas sementes do híbrido Bárbara, representadas por cinco lotes (1 a 5 na primeira etapa e por outros cinco (6 a 10 na segunda. Foram realizados os teste de germinação, primeira contagem de germinação, emergência de plântulas, índice de velocidade de emergência e de condutividade elétrica (CE. Para o teste de CE, foram estudadas variações no volume de água (50 e 75mL, na temperatura (20, 25 e 30ºC e no tempo de embebição (1, 2, 4, 8, 12, 16, 20 e 24 horas. Assim, concluiu-se que a condição mais adequada para o teste de CE é a utilização de 50 sementes em 75mL de água por oito horas, à temperatura de 25ºC.The electrical conductivity in the soaked seed solution has been used successfully to evaluate seed vigor. This work was developed with the objective of determining the adequate procedures to evaluate the electrical conductivity test of pumpkin (Cucurbita moschata Duch ex. Lam seeds. For pumpkin, hybrid Bárbara, five lots of seeds, in both stages, were used, all with quality patterns (germination above the minimum demanded by commercialization. The standard germination, first countig of germination, seedling emergency, and emergency speed index and electrical conductivity (EC test were run. For the EC test two volumes of water (50 and 75mL, three temperatures (20, 25 and 30ºC and eight period of seed soaking (1, 2, 4, 8, 12, 16, 20 and 24 hours were used. So, it can be concluded that the EC test can be conducted using 50 seeds, 25ºC, 75mL and 8h of embibition period for pumpkin seeds.

  20. Avaliação de extratos vegetais no controle de mosca-branca, Bemisia tabaci biótipo B em abóbora Evaluation of plant extracts in the control of whitefly Bemisia tabaci biotype B in squash

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    Bruno Marcus Freire Vieira Lima

    2013-09-01

    Full Text Available A abóbora (Cucurbita moschata Duch. é uma cultura de importância para a agricultura familiar da região Nordeste do Brasil. No entanto, a ocorrência do prateamento das folhas da aboboreira ocasionado pela mosca-branca, Bemisia tabaci biótipo B limita sua produção, causando prejuízos aos agricultores. O objetivo deste trabalho foi avaliar a eficiência de extratos vegetais sobre o nível populacional de ninfas de mosca-branca em abóbora cv. Jacarezinho. O experimento foi instalado em campo experimental, em blocos casualizados, constituído de seis tratamentos e três repetições. As aplicações dos extratos vegetais foram feitas com intervalo de sete dias, fazendo-se uso de substâncias extraídas das seguintes plantas: canudo (Ipomoea carnea subsp. fistulosa, mamona (Ricinus communis L., tinguí (Mascagnia rigida Griseb, cardo-santo (Argemone mexicana L., e o óleo comercial Natuneem®. Todos os extratos vegetais obtiveram eficiência no controle de ninfas B. tabaci, sendo o R. communis (75,49%, M. rigida (73,99%, I. carnea (72,24%, óleo de nim (70,4% e A. mexicana (69,16%.The squash (Cucurbita moschata Duch. is an important crop for family farmers in the northeast of Brazil. However, the occurrence of leaf silvering caused by the whitefly, Bemisia tabaci biotype B, limits production, causing losses to farmers. The aim of this study was to evaluate the efficiency of plant extracts on the population levels of whitefly nymphs on the squash cv. Jacarezinho. The experiment was conducted at an experimental field, in a randomized block design consisting of six treatments and three replications. Applications of plant extracts were made at an interval of seven days, making use of substances extracted from the following plants: Ipomoea carnea subsp. Fistulosa, castor bean (Ricinus communis L., Tingui (Mascagnia rigida Griseb, cardo-santo (Argemone mexicana L., and the commercial oil product Natuneem ®. All of the plant extracts were

  1. O teste de frio sem solo em sementes de abóbora The modified cold germination test in squash seeds

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    Derblai Casaroli

    2006-12-01

    Full Text Available Este trabalho teve como objetivo determinar o período de exposição à baixa temperatura no teste de frio sem solo, para avaliação de diferentes potenciais fisiológicos em lotes de sementes de abóbora. Foram avaliados seis lotes de sementes de abóbora cultivar "Menina Brasileira" (Cucurbita moschata Duch. produzidas na safra 2003/2004. As sementes foram submetidas a diferentes períodos (três, cinco, sete e nove dias de exposição à baixa temperatura para condução do teste de frio sem solo, a uma temperatura constante de 10°C. Utilizou-se o delineamento inteiramente casualizado, em que os tratamentos constituíram um fatorial 6x4 (seis lotes e quatro períodos de exposição, com quatro repetições de 50 sementes. As médias foram comparadas pelo teste de Tukey em nível de 5% de probabilidade de erro. Realizou-se também o teste de correlação simples de Pearson .Os resultados permitiram concluir que o período de três dias de exposição à baixa temperatura foi o mais indicado para a condução do teste de frio sem solo em sementes de abóbora.This research was aimed at determining the exposition period at low temperature in the modified cold germination test, to evaluate physiological potential of squash seed lots. Six seed lots of squash, variety Menina Brasileira (Cucurbita moschata Duch., produced in 2003/2004, were submitted at different exposition times for the development of the cold test. The modified cold gernation test was carried out at 10°C of constant temperature, during exposition time of three, five, seven and nine days. A completely randomized desing was used to the cold test, 0 constituting a factorial 6x4, corresponding to the six seed lots and four exposition time (3, 5, 7, and 9 days, with four repetitions. The averages were compared by the Tukey test, at 5% of probability. The correlation test of Person (r was used too. For the modified cold germination test, the period of three days was the most

  2. The Effect of Different Cucurbit Rootstocks on Some Morphological and Physiological Traits of Cucumber (Cucumis sativus cv. Super Dominus

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    reihane Mesgari

    2017-02-01

    Full Text Available Introduction: Cucumber is one of the most important vegetable crops for the local consumption and exportation. The use of grafted vegetable seedlings has been popular in many countries during recent years. Growing fruit-bearing vegetables, chiefly tomato, cucumber and watermelon through grafted seedlings become a widespread practice worldwide. Grafting is a valuable technique to avoid soil-borne diseases, provide biotic and abiotic stress tolerance, enhance nutrient uptake, optimize water use, and increase fruit yield and quality. Vegetable grafting is a new topic in Iran and there are a limited number of studies on grafted vegetable production. However, attention to grafting by researchers has recently increased. Suitable rootstocks should be identified and characterized for the effective utilization of grafting. The rootstock's vigorous root system increases the efficiency of water and nutrient absorption, and may also serve as a source of endogenous plant hormones, thus leading to increased growth and yield in addition to disease control. In the present study, we investigated the response of two Cucurbita sp. and an Iranian melon as rootstocks for cucumber. Materials and methods: In order to study the effect of cucurbit rootstocks and grafting method on growth, yield and fruit quality of cucumber (Cucumis sativus cv. Super Dominus, an experiment was conducted as a factorial design in the base of RCBD with three replications in the greenhouse and research farm, University of Zanjan. Treatments were included three rootstocks (Cucurbita moschata L., Lagenaria siceraria and Cucumis melo L. and ungrafted plants (control and two grafting method (hole insertion and splice grafting. Seeds were sown simultaneously in plastic pots. For obtaining the same stem diameter of scion and rootstocks, cucumber seeds were planted four days earlier than rootstocks seeds. The seedlings were grown in an environment-controlled greenhouse with 25/20 day

  3. Control biológico de enfermedades mediante el tratamiento de semillas de cucurbitáceas con rizobacterias promotoras del crecimiento de las plantas (PGPR./Biological control of diseases by cucurbits seeds treatment with rhizobacteria plant growth promoting (PGPR.

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    Gilda Jiménez Montejo

    2014-01-01

    Full Text Available Resumen:Las regulaciones sobre la producción de alimentos sanos limitan actualmente el uso de fungicidas sintéticos para el tratamiento de semillas y, por otra parte, no se dispone de bactericidas efectivos. Se condujeron experimentos en condiciones controladas para evaluar el efecto de las cepas de rizobacterias antagonistas Bacillus subtilis F16/95, B. subtilis Xph y Pseudomonas putida 14A en el control biológico de la Mancha bacteriana de las cucurbitáceas, la Mancha bacteriana del fruto de melón y la pudrición de la raíz, provocadas por Xanthomonas cucurbitae, Acidovorax avenae subsp. citrulli y Fusarium oxysporum, respectivamente. Se realizó el tratamiento de semillas de pepino (C. sativus, L. var. Poinsett, calabaza (Cucurbita moschata, Duch. var. INIVIT C-88, y melón de Castilla (Cucumis melo, L. var. Hale’s Best. Los tratamientos biológicos mostraron una reducción en la incidencia de las enfermedades e incrementos de longitud del tallo y peso fresco de hojas y raíces, en todas las interacciones planta-patógeno-antagonista. La colonización de raíces alcanzó rangos de 8,9-8,0 y 7,8-7,0 log (ufc cm-1 de raíz a los 4 y 15 días después de la siembra. Estos resultados caracterizaron las cepas aplicadas como rizobacterias promotoras del crecimiento vegetal (PGPR y aconsejan la realización de ensayos de campo./Abstract:Nowadays the regulations about the production of healthy foods limit the use of synthetic fungicides for seeds treatment and, on the other hand, effective bactericides are not available. Experiments under controlled conditions were conducted to evaluate the effect of rhizobacterial antagonistic strains Bacillus subtilis F16/95, B. subtilis Xph and Pseudomonas putida 14A on the biological control of Cucurbits bacterial spot, Watermelon bacterial fruit blotch and Fusarium root rot caused by Xanthomonas cucurbitae, Acidovorax avenae subsp. citrulli and Fusarium oxysporum, respectively. Cucumber (Cucumis sativus

  4. 基于外壳蛋白基因序列对3种葫芦科作物病毒的分子分析%Molecular analysis of three cucurbit viruses based on coat protein gene sequences

    Institute of Scientific and Technical Information of China (English)

    古勤生; 田延平; 彭斌; 刘丽锋; 邓丛良; 梁新苗; 孟娟

    2008-01-01

    从山西运城的南瓜(Cucurbita moschata)、河南开封和孟津的西葫芦(Cucurbita pepo)上通过ELISA分别检测到南瓜花叶病毒(Squash mosaic virus,SqMV)、西瓜花叶病毒(Watermelon mosaic virus,WMV)和番木瓜环斑病毒西瓜株系(Papa-ya ringspot virus-Watermelon type,PRSV-W),通过接种分离纯化获得其病毒毒原(CH99/211,CH99/113,CH99/69).本研究克隆了这3个分离物的外壳蛋白基因,应用DNAStar、Clustal X、Phylip等分析软件将这些序列同已报道的相应序列进行分析并构建系统树.结果发现SqMV可以分为两个组,CH99/211分离物与美国的Arizona分离物接近,应属于Z组;WMV分为两个组,CH99/69同国内黑龙江分离物(WMV-HLJ)和云南分离物(WMV-CHN)最接近,成一小簇;PRSV分成两个组,CH99/113属于Ⅱ组,与国内SP、MZ和BN分离物接近.序列分析表明PRSV和WMV在进化上具有地理相关性.SqMV和PRSV-W外壳蛋白基因序列分析属国内首次报道.

  5. Host Reaction of Watermelon mosaic virus Isolates Infecting Melon from Different Geographical Origins in Xinjiang of China

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    Dong WANG

    2017-01-01

    Full Text Available Watermelon mosaic virus (WMV is one of the major viruses infecting cucurbit crops worldwide. Although WMV is very common worldwide, little is known about the biological traits of WMV isolates from China. Hence, this study aimed to characterize 11 WMV isolates infecting melon from different geographical origins in Xinjiang based on experimental hosts. Sap inoculation of the 11 WMV isolates onto a range of 13 plant species revealed some differences compared to the WMV isolates collected from other countries. Our results showed that, overall, there were no obvious correlations of host responses to inoculation with WMV isolates from different geographical origins. However, isolate JS-1 caused mild mosaic on Cucurbita moschata, whereas the remaining 10 isolates were asymptomatic on this plant species. Moreover, in Datura stramonium, isolate TYG-1 induced mosaic, whereas the remaining 10 isolates did not infect this species. All isolates infected systemically Cucurbita pepo and Cucumis melo plants, causing severe symptoms. All isolates did not induce any symptoms on Cucumis sativus, but the virus could be detected using RT-PCR. Additionally, all isolates infected systemically Nicotiana tabacum plants, causing mild mosaics. Chenopodium amaranticolor and Chenopodium quinoa reacted to all isolates by chlorotic local lesions in the inoculated leaves, and the virus was detected in the inoculated leaves using RT-PCR. In addition, the attempts to transmit the isolates to Luffa cylindrica, Vicia faba, Phaseolus vulgaris, Vigna unguiculata or Pisum sativum failed as confirmed by negative RT-PCR. Our results would be useful for understanding the biological variability of WMV.

  6. Composition analysis and the application to pork emulsion sausage of three kinds of pumpkin powders%3种南瓜粉成分分析及其在猪肉乳化香肠中的应用研究

    Institute of Scientific and Technical Information of China (English)

    滕安国; 贾鸿茭; 王稳航

    2016-01-01

    南瓜富含多糖、蛋白质,是一种优良的膳食纤维来源.首先对密本南瓜(Cucurbita moschata)、窝瓜(Cucubita maxima)和美洲南瓜(Cucurbita pepo)的常规成分及加工性能进行了研究.经检测,3种南瓜粉总膳食纤维含量分别为20.81%、52.83%、46.28%,其吸油性介于4.59~5.63g/g,吸水性介于为8.85~11.26 g/g.分别以1%、3%、5%的比例将南瓜粉添加到猪肉乳化香肠中,结果发现:3种南瓜粉都随着添加量的增大而使乳化香肠的蒸煮损失率显著降低(p<0.05);香肠硬度、弹性、咀嚼性和黏聚性逐渐增大;亮度(L*)减小,红度(a*)和黄度(b*)增大;pH变化不显著.南瓜粉可作为一种潜在的高膳食纤维配料应用于功能性肉制品的开发.

  7. A Research on the Relationship between GPAT and Plant Chill-Resistance%甘油-3磷酸转酰酶氨基酸与植物抗冷性关系初探

    Institute of Scientific and Technical Information of China (English)

    杨明挚; 陈善娜; 鄢波; 黄兴奇; 刘继梅

    2000-01-01

    甘油-3磷酸转酰酶(GPAT)与植物抗冷性密切相关.南瓜(Cucurbita moschata)与黑子南瓜(Cucurbita ficifolia)同属不同种,亲缘关系较近,但却存在显著的抗冷性差异.南瓜及黑子南瓜GPAT基因的克隆,可以使我们从二者推导的有限氨基酸的差异中对GPAT氨基酸组成及其与植物抗冷性作一定的探讨.发现在南瓜与黑子南瓜13个不同的氨基酸残基中有3个与抗冷性植物拟南芥菜(Arabidopsis thaliana)、豌豆(Pisum sativum)、红花(Carthamus tinctorius)和菠菜(Spinacia oleracea)等相同,可能与黑子南瓜比南瓜更具抗冷性的原因有关.比较南瓜、黑子南瓜、豌豆、红花、拟南芥菜和菠菜等植物中GPAT基因推导的氨基酸序列发现,在比较抗冷的拟南芥菜、红花、豌豆和菠菜等植物中,虽然它们之间的亲缘关系都比较远,但某些位点上的氨基酸残基却完全相同,而与南瓜等抗冷性较差的植物不同,这些位点的氨基酸残基可能也与GPAT对底物酰基的选择性有关.

  8. Effects of grafting with pumpkin rootstock on carbohydrate metabolism in cucumber seedlings under Ca(NO3)2 stress.

    Science.gov (United States)

    Xing, Wen-wen; Li, Lin; Gao, Pan; Li, He; Shao, Qiao-sai; Shu, Sheng; Sun, Jin; Guo, Shi-rong

    2015-02-01

    This study investigated the effects of grafting on the carbohydrate status and the enzymes of carbohydrate metabolism in self-grafted and grafted cucumber seedlings using the salt-tolerant pumpkin rootstock 'Qingzhen 1' (Cucurbita maxima × Cucurbita moschata) under 80 mM Ca(NO3)2 stress for 6 d. The growth of self-grafted seedlings was significantly inhibited after the treatment of Ca(NO3)2 stress, whereas the inhibition of growth was alleviated in pumpkin rootstock-grafted seedlings. Ca(NO3)2 stress increased the contents of the total soluble sugar, sucrose and fructose, but decreased the starch content in rootstock-grafted leaves. However, compared with self-grafted plants, rootstock-grafted seedlings were observed with a higher content of sucrose and total soluble sugar (TSS) under salt stress. Rootstock-grafted seedlings exhibited higher activities of acid invertase (AI), neutral invertase (NI) and phosphate sucrose synthase (SPS) of sucrose metabolism in leaves than that of self-grafted seedlings under salinity. Moreover, the activities of fructokinase (FK), hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK) of glycolysis were maintained at a higher level in leaves of rootstock-grafted seedlings after Ca(NO3)2 stress. Additionally, rootstock-grafting decrease the high percentage enhancement of key enzymes gene expression in glycolysis in the scion leaves of cucumber seedlings induced by salt stress. These results suggest that the rootstock-grafting improved salt tolerance, which might play a role in elevated sucrose metabolism and a glycolytic pathway regulated by the pumpkin rootstock.

  9. Distinct genetic lineages of Bactrocera caudata (Insecta: Tephritidae revealed by COI and 16S DNA sequences.

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    Phaik-Eem Lim

    Full Text Available The fruit fly Bactrocera caudata is a pest species of economic importance in Asia. Its larvae feed on the flowers of Cucurbitaceae such as Cucurbita moschata. To-date it is distinguished from related species based on morphological characters. Specimens of B. caudata from Peninsular Malaysia and Indonesia (Bali and Lombok were analysed using the partial DNA sequences of cytochrome c oxidase subunit I (COI and 16S rRNA genes. Both gene sequences revealed that B. caudata from Peninsular Malaysia was distinctly different from B. caudata of Bali and Lombok, without common haplotype between them. Phylogenetic analysis revealed two distinct clades, indicating distinct genetic lineage. The uncorrected 'p' distance for COI sequences between B. caudata of Malaysia-Thailand-China and B. caudata of Bali-Lombok was 5.65%, for 16S sequences from 2.76 to 2.99%, and for combined COI and 16S sequences 4.45 to 4.46%. The 'p' values are distinctly different from intraspecific 'p' distance (0-0.23%. Both the B. caudata lineages are distinctly separated from related species in the subgenus Zeugodacus - B. ascita, B. scutellata, B. ishigakiensis, B. diaphora, B. tau, B. cucurbitae, and B. depressa. Molecular phylogenetic analysis indicates that the B. caudata lineages are closely related to B. ascita sp. B, and form a clade with B. scutellata, B. ishigakiensis, B. diaphora and B. ascita sp. A. This study provides additional baseline for the phylogenetic relationships of Bactrocera fruit flies of the subgenus Zeugodacus. Both the COI and 16S genes could be useful markers for the molecular differentiation and phylogenetic analysis of tephritid fruit flies.

  10. Distinct genetic lineages of Bactrocera caudata (Insecta: Tephritidae) revealed by COI and 16S DNA sequences.

    Science.gov (United States)

    Lim, Phaik-Eem; Tan, Ji; Suana, I Wayan; Eamsobhana, Praphathip; Yong, Hoi Sen

    2012-01-01

    The fruit fly Bactrocera caudata is a pest species of economic importance in Asia. Its larvae feed on the flowers of Cucurbitaceae such as Cucurbita moschata. To-date it is distinguished from related species based on morphological characters. Specimens of B. caudata from Peninsular Malaysia and Indonesia (Bali and Lombok) were analysed using the partial DNA sequences of cytochrome c oxidase subunit I (COI) and 16S rRNA genes. Both gene sequences revealed that B. caudata from Peninsular Malaysia was distinctly different from B. caudata of Bali and Lombok, without common haplotype between them. Phylogenetic analysis revealed two distinct clades, indicating distinct genetic lineage. The uncorrected 'p' distance for COI sequences between B. caudata of Malaysia-Thailand-China and B. caudata of Bali-Lombok was 5.65%, for 16S sequences from 2.76 to 2.99%, and for combined COI and 16S sequences 4.45 to 4.46%. The 'p' values are distinctly different from intraspecific 'p' distance (0-0.23%). Both the B. caudata lineages are distinctly separated from related species in the subgenus Zeugodacus - B. ascita, B. scutellata, B. ishigakiensis, B. diaphora, B. tau, B. cucurbitae, and B. depressa. Molecular phylogenetic analysis indicates that the B. caudata lineages are closely related to B. ascita sp. B, and form a clade with B. scutellata, B. ishigakiensis, B. diaphora and B. ascita sp. A. This study provides additional baseline for the phylogenetic relationships of Bactrocera fruit flies of the subgenus Zeugodacus. Both the COI and 16S genes could be useful markers for the molecular differentiation and phylogenetic analysis of tephritid fruit flies.

  11. Wheat Bread with Pumpkin (Cucurbita maxima L. Pulp as a Functional Food Product

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    Renata Różyło

    2014-01-01

    Full Text Available In this study, a new application of pumpkin pulp in bread production is shown. The aim of this work is to determine the influence of the addition of fresh pumpkin pulp directly into wheat flour on physical, sensorial and biological properties of bread. The bioaccessibility of active compounds was also studied. An increase in the addition of pumpkin pulp from 5 to 20 % (converted to dry matter caused a decrease of bread volume and increase of crumb hardness and cohesiveness. The sensory characteristics of the bread showed that a partial replacement of wheat fl our with up to 10 % of pumpkin pulp gave satisfactory results. The taste, aroma and overall acceptability of control bread and bread containing 5 or 10 % of pulp had the highest degree of liking. The addition of higher levels of pumpkin pulp caused an unpleasant aroma and taste. Pumpkin pulp is a good material to complement the bread with potentially bioaccessible phenolics (including flavonoids and, especially, with peptides. The highest antioxidant activity was observed, in most cases, of the samples with added 10 and 15 % of pumpkin pulp. The addition of the pulp significantly enriched the bread with potentially bioaccessible angiotensin-converting enzyme (ACE inhibitors. The highest activity was determined in the bread with 15 and 20 % pumpkin pulp. ACE inhibitors from the tested bread were highly bioaccessible in vitro. Pumpkin pulp seems to be a valuable source of active compounds to complement the wheat bread. Adding the pulp directly to the wheat flour gives satisfactory baking results and reduces the cost of production. Additionally, pumpkin pulp is sometimes treated as waste material after the acquisition of seeds, thus using it as bread supplement also has environmental and economic benefi ts.

  12. Health hazards and heavy metals accumulation by summer squash (Cucurbita pepo L.) cultivated in contaminated soils.

    Science.gov (United States)

    Galal, Tarek M

    2016-07-01

    The present study was carried out to investigate the heavy metal concentration accumulated by summer squash cultivated in contaminated soil and their health hazards for public consumers at south Cairo Province, Egypt. Soil and plants were sampled from contaminated and reference farms, using 1 m(2) quadrats, for biomass estimation and nutrient analysis. The daily intake of metals (DIM) and health risk index (HRI) were estimated. Significant differences in soil variables (except As) between contaminated and reference sites were recognized. Summer squash showed remarkable reduction in fresh and dry biomass, fruit production, and photosynthetic pigments under pollution stress. The inorganic and organic nutrients in the aboveground and belowground parts showed significant reduction in contaminated site. In addition, higher concentrations of heavy metals were accumulated in the edible parts and roots more than shoots. The bioaccumulation factor of summer squash for investigated metals was greater than 1, while the translocation factor did not exceed unity in both contaminated and reference sites. The DIM for all investigated metals in the reference site and in the contaminated site (except Fe and Mn) did not exceed 1 in both adults and children. However, HRI of Ni and Mn in the reference site and Pb, Cd, Cu, Ni, Fe, Mn, and Zn in the contaminated one exceeded unity indicating great potential to pose health risk to the consumers. The author recommends that people living in the contaminated area should not eat large quantities of summer squash, so as to avoid excess accumulation of heavy metals in their bodies.

  13. Screening of Furanone in Cucurbita melo and Evaluation of its Bioactive Potential Using In Silico Studies.

    Science.gov (United States)

    Singh, Nidhi; Sudandiradoss, C; Abraham, Jayanthi

    2016-12-01

    The work presented here attempts to screen for the presence of 4-Hydroxy-2,5-dimethyl-3(2H)-furanone in fruits and also any bioactive potential present in the fruit extracts. Curcurbita melo was selected for the study, and the fruit was crushed, filtered and extracted with ethyl acetate as the solvent. The resulting extract was subjected to disk diffusion test using Kirby Bauer method for checking its antimicrobial potential. Melon extract showed promising results against different clinical pathogens, 19-mm zone being the largest against Klebsiella pneumoniae and 17 mm against Shigella dysenteriae. GC-MS data of the melon extract confirmed the existence of furanone derivative in the extract. To evaluate the antimicrobial activity, in silico studies were performed using AutoDock 4.0 software, and topoisomerase was used as the target protein molecule for the isolated compound and 3-methyl-2-(2-oxopropyl)furan as the ligand. Further, the results were interpreted using PyMol and Ligplot plus softwares. The results confirmed the presence of molecular interactions between the protein molecule and the ligand. It can be envisaged that these interactions are responsible for the inhibitory effects of the extract .

  14. Influence of different fertilizer types of zucchini (Cucurbita pepo) on the structure of nematode communities.

    Science.gov (United States)

    Haytova, D; Bileva, T

    2011-01-01

    Increasing efficiency of production of vegetable crops is directly related to search for appropriate solution to increase their productivity. Organic amendments have been used for centuries to improve soil fertility and crop yield. Our study suggests that organic amendments can also be used as nematicidal agents. The survey was conducted on Experimental field of Department Horticulture at Agricultural University of Plovdiv, Bulgaria in 2009 on nematode infested sites. Combination with two types of fertilizers was used to investigate their effects on the community of soil nematodes. Characterization and comparative analysis among treatments of soil nematode community structure based on different ecological measures such as total nematode abundance, number of genera, trophic diversity and etc., was made. Changes in the composition and structure of nematode community as result of different fertilizer types were assessed.

  15. Ammonium-related metabolic changes affect somatic embryogenesis in pumpkin (Cucurbita pepo L.).

    Science.gov (United States)

    Mihaljević, Snježana; Radić, Sandra; Bauer, Nataša; Garić, Rade; Mihaljević, Branka; Horvat, Gordana; Leljak-Levanić, Dunja; Jelaska, Sibila

    2011-11-01

    Somatic embryogenesis in pumpkin can be induced on auxin-containing medium and also on hormone-free medium containing 1mM ammonium (NH(4)(+)) as the sole source of nitrogen. Growth of NH(4)(+)-induced embryogenic tissue was slow and caused considerable acidification of the culture medium. Small spherical cells with dense cytoplasma formed proembryogenic cell clusters that could not develop into late stage embryos. Buffering of NH(4)(+) medium with 25mM 2-(N-morpholino)-ethane-sulfonic acid enhanced tissue proliferation, but no further differentiation was observed. Later stage embryos developed only after re-supply of nitrogen in form of nitrate or l-glutamine. Effects of nitrogen status and pH of culture media on ammonium assimilation were analyzed by following the activity of glutamine synthetase (GS) in relation to phenylalanine ammonia-lyase (PAL). Increased activity of GS and PAL in NH(4)(+) induced tissue coincided with significantly higher activity of stress-related enzymes superoxide dismutase (SOD) and soluble peroxidase (POD), indicating oxidative stress response of embryogenic tissue to NH(4)(+) as the sole source of nitrogen. In addition, considerable increase was observed in callose accumulation and esterase activity, the early markers of somatic embryogenesis. Activity of stress-related enzymes decreased after the re-supply of nitrate (20mM) or Gln (10mM) in combination with NH(4)(+) (1mM), which subsequently triggered globular embryo development. Together, these results suggest that stress responses, as affected by nitrogen supply, contribute to the regulation of embryogenic competence in pumpkin.

  16. Effect of phosphorylation on antioxidant activities of pumpkin (Cucurbita pepo, Lady godiva) polysaccharide.

    Science.gov (United States)

    Song, Yi; Ni, Yuanying; Hu, Xiaosong; Li, Quanhong

    2015-11-01

    Phosphorylated derivatives of pumpkin polysaccharide with different degree of substitution were synthesized using POCl3 and pyridine. Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems. Results showed that high ratio of POCl3/pyridine could increase the degree of substitution and no remarkable degradation occurred in the phosphorylation process. Characteristic absorption of phosphorylation appeared both in the IR and (31)P NMR spectrum. The df values between 2.27 and 2.55 indicated the relatively expanded conformation of the phosphorylated derivatives. All the phosphorylated polysaccharides exhibited higher antioxidant activities. H2O2-induced oxidative damages on rat thymic lymphocyte were also prevented by the derivatives. In general, phosphorylation could improve the antioxidant activities of pumpkin polysaccharide both in vitro and in a cell system.

  17. Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin.

    Science.gov (United States)

    Wyatt, Lindsay E; Strickler, Susan R; Mueller, Lukas A; Mazourek, Michael

    2016-01-01

    Both the fruit mesocarp and the seeds of winter squash can be used for consumption, although the focus of breeding efforts varies by cultivar. Cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content, percent dry matter, and percent soluble solids, while these traits are essentially ignored in oilseed pumpkins. To compare fruit development in these two types of squash, we sequenced the fruit transcriptome of two cultivars bred for different purposes: an acorn squash, 'Sweet REBA', and an oilseed pumpkin, 'Lady Godiva'. Putative metabolic pathways were developed for carotenoid, starch, and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the pathways. Gene expression, especially of known rate-limiting and branch point genes, corresponded with metabolite accumulation both across development and between the two cultivars. Thus, developmental regulation of metabolite genes is an important factor in winter squash fruit quality.

  18. Application of Asian pumpkin (Cucurbita ficifolia) serine proteinase for production of biologically active peptides from casein.

    Science.gov (United States)

    Dąbrowska, Anna; Szołtysik, Marek; Babij, Konrad; Pokora, Marta; Zambrowicz, Aleksandra; Chrzanowska, Józefa

    2013-01-01

    The main objective of this study was to determine potential application of a serine proteinase derived from Asian pumpkin for obtaining biologically active peptides from casein. The course of casein hydrolysis by three doses of the enzyme (50, 150, 300 U/mg of protein) was monitored for 24 hours by the determinations of: hydrolysis degree DH (%), free amino group content (μmole Gly/g), RP HPLC peptide profiles and by polyacrylamide gel electrophoresis. In all hydrolyzates analyzed antioxidant activities were determined using three tests: the ability to reduce iron ions in FRAP test, the ability to scavenge free radicals in DPPH test, and Fe(2+) chelating activity. The antimicrobial activity of obtained peptide fractions was determined as the ability to inhibit the growth of Escherichia coli, Bacillus cereus and Pseudomonas fluorescens in a diffusion plate test. The deepest degradation, expressed as the DH [%] and the free amino group content (67% and 7528 µmole Gly/mg, respectively), was noted in samples hydrolyzed with 300 U/ml of enzyme for 24 hours, while in other samples the determined values were about three and two times lower. The results were in agreement with the peptide profiles obtained by RP HPLC. The highest antioxidative activities determined in all tests were seen for the casein hydrolysate obtained with 300 U/mg protein of serine proteinase after 24 h of reaction (2.15 µM Trolox/mg, 96.15 µg Fe(3+)/mg, 814.97 µg Fe(2+)/mg). Antimicrobial activity was presented in three preparations. In other samples no antimicrobial activity was detected.

  19. Structural diversity of pectins isolated from the Styrian oil-pumpkin (Cucurbita pepo var. styriaca) fruit.

    Science.gov (United States)

    Košťálová, Zuzana; Hromádková, Zdenka; Ebringerová, Anna

    2013-03-01

    To evaluate the seeded fruit biomass of the Styrian oil-pumpkin in view of its pectin component, a series of acidic polysaccharides were isolated by a six-step sequential extraction using hot water, EDTA, dilute HCl (twice) and dilute and stronger NaOH solutions. Chemical, physicochemical and spectroscopy analyses revealed that the first four fractions comprised partially methyl-esterified and acetylated pectins with varying proportions of rhamnogalacturonan regions ramified with galactose- and arabinose-containing side chains and showed considerable polymolecularity. The alkali-extracted polysaccharides contained lower amounts of pectins with homogalacturonan and arabinose-rich rhamnogalacturonan regions next to hemicelluloses prevailing in the last polysaccharide. Using (1)H-(13)C HSQC and HMBC spectroscopy, the resonances of free and methylesterified galacturonic acid residues in the purified acid-extracted pectin were unambiguously established and various diads formed by both residues identified. The results might serve as a basis for searching technological conditions to produce pectin from the oil-pumpkin fruit biomass.

  20. Wheat Bread with Pumpkin (Cucurbita maxima L.) Pulp
as a Functional Food Product.

    Science.gov (United States)

    Różyło, Renata; Gawlik-Dziki, Urszula; Dziki, Dariusz; Jakubczyk, Anna; Karaś, Monika; Różyło, Krzysztof

    2014-12-01

    In this study, a new application of pumpkin pulp in bread production is shown. The aim of this work is to determine the influence of the addition of fresh pumpkin pulp directly into wheat flour on physical, sensorial and biological properties of bread. The bioaccessibility of active compounds was also studied. An increase in the addition of pumpkin pulp from 5 to 20% (converted to dry matter) caused a decrease of bread volume and increase of crumb hardness and cohesiveness. The sensory characteristics of the bread showed that a partial replacement of wheat flour with up to 10% of pumpkin pulp gave satisfactory results. The taste, aroma and overall acceptability of control bread and bread containing 5 or 10% of pulp had the highest degree of liking. The addition of higher levels of pumpkin pulp caused an unpleasant aroma and taste. Pumpkin pulp is a good material to complement the bread with potentially bioaccessible phenolics (including flavonoids) and, especially, with peptides. The highest antioxidant activity was observed, in most cases, of the samples with added 10 and 15% of pumpkin pulp. The addition of the pulp significantly enriched the bread with potentially bioaccessible angiotensin-converting enzyme (ACE) inhibitors. The highest activity was determined in the bread with 15 and 20% pumpkin pulp. ACE inhibitors from the tested bread were highly bioaccessible in vitro. Pumpkin pulp seems to be a valuable source of active compounds to complement the wheat bread. Adding the pulp directly to the wheat flour gives satisfactory baking results and reduces the cost of production. Additionally, pumpkin pulp is sometimes treated as waste material after the acquisition of seeds, thus using it as bread supplement also has environmental and economic benefits. Key words: pumpkin, bread, texture, antioxidants, bioaccessibility in vitro, angiotensin-converting enzyme (ACE) inhibition.

  1. Effect of NPK fertilizer on chemical composition of pumpkin (Cucurbita pepo Linn.) seeds.

    Science.gov (United States)

    Oloyede, F M; Obisesan, I O; Agbaje, G O; Obuotor, E M

    2012-01-01

    An investigation of the proximate composition and antioxidant profile of pumpkin seeds obtained from different levels of NPK 15 : 15 : 15 compound fertilizer application at the Obafemi Awolowo University, Ile-Ife, Nigeria was carried out. Pumpkin seeds were grown in 2010 for two cropping seasons (May to August and August to November), and the following fertilizer rates were applied: 0, 50, 100, 150, 200, and 250 kg/ha. Standard analytical methods were used to determine protein, crude fibre, ash, fat, carbohydrate, antioxidant activities, phenol, flavonoid, proanthocyanidin, and anthocyanin. The highest concentrations of the proximate and antioxidants analysed were found from the seeds of control and those treated with lower NPK rates. The mean protein, ash, crude fibre, and carbohydrate values of pumpkin seeds at zero to 100 kg NPK/ha were 27%, 1.56%, 0.56%, and 11.7% respectively. At these same levels of fertilizer, pumpkin seed oil yield was 59%. Antioxidant activities ranged from 89.9 to 90.4% while total phenol was 47 mg/100 g. Except for carbohydrate, the % concentration of nutrients and antioxidants in pumpkin seeds was significantly (P = 0.05) depressed with fertilizer rates above 100 g/ha.

  2. Wheat Bread with Pumpkin (Cucurbita maxima L.) Pulp
as a Functional Food Product

    Science.gov (United States)

    Gawlik-Dziki, Urszula; Dziki, Dariusz; Jakubczyk, Anna; Karaś, Monika; Różyło, Krzysztof

    2014-01-01

    Summary In this study, a new application of pumpkin pulp in bread production is shown. The aim of this work is to determine the influence of the addition of fresh pumpkin pulp directly into wheat flour on physical, sensorial and biological properties of bread. The bioaccessibility of active compounds was also studied. An increase in the addition of pumpkin pulp from 5 to 20% (converted to dry matter) caused a decrease of bread volume and increase of crumb hardness and cohesiveness. The sensory characteristics of the bread showed that a partial replacement of wheat flour with up to 10% of pumpkin pulp gave satisfactory results. The taste, aroma and overall acceptability of control bread and bread containing 5 or 10% of pulp had the highest degree of liking. The addition of higher levels of pumpkin pulp caused an unpleasant aroma and taste. Pumpkin pulp is a good material to complement the bread with potentially bioaccessible phenolics (including flavonoids) and, especially, with peptides. The highest antioxidant activity was observed, in most cases, of the samples with added 10 and 15% of pumpkin pulp. The addition of the pulp significantly enriched the bread with potentially bioaccessible angiotensin-converting enzyme (ACE) inhibitors. The highest activity was determined in the bread with 15 and 20% pumpkin pulp. ACE inhibitors from the tested bread were highly bioaccessible in vitro. Pumpkin pulp seems to be a valuable source of active compounds to complement the wheat bread. Adding the pulp directly to the wheat flour gives satisfactory baking results and reduces the cost of production. Additionally, pumpkin pulp is sometimes treated as waste material after the acquisition of seeds, thus using it as bread supplement also has environmental and economic benefits. Key words: pumpkin, bread, texture, antioxidants, bioaccessibility in vitro, angiotensin-converting enzyme (ACE) inhibition PMID:27904316

  3. Functional properties of pumpkin (Cucurbita pepo seed protein isolate and hydrolysate

    Directory of Open Access Journals (Sweden)

    Bučko Sandra Đ.

    2016-01-01

    Full Text Available Pumpkin seed protein isolate (PSPI was enzymatically hydrolysed by pepsin to obtain pumpkin seed protein hydrolysate, PSPH. Investigation on solubility, interfacial and emulsifying properties of both PSPI and PSPH was conducted under different conditions of pH (3-8 and ionic strength (0-1 mol/dm3 NaCl. PSPI had the lowest solubility, i.e. isoelectric point (pI, at pH 5. PSPH had higher solubility than PSPI over whole range of pH and ionic strengths tested. Decrease in surface and interfacial tension evidenced that both PSPI and PSPH adsorb at air/protein solution and oil/protein solution interface. Emulsions (20 % oil in water stabilized by 1 g/100cm3 PSPI or PSPH solution were prepared at pH 3, 5 and 8 and ionic strength of 0 and 0.5 mol/dm3 NaCl. PSPH stabilized emulsions from coalescence at all pH and ionic strengths tested. PSPI was able to stabilize emulsions at pH 3 and 0 mol/dm3 NaCl, and at pH 8 regardless of ionic strength, while emulsions at pH 5 and both 0 and 0.5 mol/dm3 NaCl and at pH 3 when ionic strength was increased separated to oil and serum layer immediately after preparation. All emulsions were susceptible to creaming instability. [Projekat Ministarstva nauke Republike Srbije, br. III 46010

  4. Changes in carbohydrate content in zucchini fruit (Cucurbita pepo L.) under low temperature stress.

    Science.gov (United States)

    Palma, Francisco; Carvajal, Fátima; Lluch, Carmen; Jamilena, Manuel; Garrido, Dolores

    2014-03-01

    The postharvest handling of zucchini fruit includes low-temperature storage, making cold stress unavoidable. We have investigated the changes of soluble carbohydrates under this stress and its relation with weight loss and chilling injury in zucchini fruit during postharvest storage at 4 °C and 20 °C for up to 14 days. Two varieties with different degrees of chilling tolerance were compared: Natura, the more tolerant variety, and Sinatra, the variety that suffered more severe chilling-injury symptoms and weight loss. In both varieties, total soluble carbohydrates, reducing soluble carbohydrates and polyols content was generally higher during storage at 4 °C than at 20 °C, thus these parameters are related to the physiological response of zucchini fruit to cold stress. However, the raffinose content increased in Natura and Sinatra fruits during storage at 4 °C and 20 °C, although at 20 °C the increase in raffinose was more remarkable than at 4 °C in both varieties, so that the role of raffinose could be more likely related to dehydration than to chilling susceptibility of zucchini fruit. Glucose, fructose, pinitol, and acid invertase activity registered opposite trends in both varieties against chilling, increasing in Natura and decreasing in Sinatra. The increase in acid invertase activity in Natura fruit during cold storage could contribute in part to the increase of these reducing sugars, whose metabolism could be involved in the adaptation to postharvest cold storage. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  5. Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin

    Science.gov (United States)

    Wyatt, Lindsay E; Strickler, Susan R; Mueller, Lukas A; Mazourek, Michael

    2016-01-01

    Both the fruit mesocarp and the seeds of winter squash can be used for consumption, although the focus of breeding efforts varies by cultivar. Cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content, percent dry matter, and percent soluble solids, while these traits are essentially ignored in oilseed pumpkins. To compare fruit development in these two types of squash, we sequenced the fruit transcriptome of two cultivars bred for different purposes: an acorn squash, ‘Sweet REBA’, and an oilseed pumpkin, ‘Lady Godiva’. Putative metabolic pathways were developed for carotenoid, starch, and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the pathways. Gene expression, especially of known rate-limiting and branch point genes, corresponded with metabolite accumulation both across development and between the two cultivars. Thus, developmental regulation of metabolite genes is an important factor in winter squash fruit quality. PMID:27688889

  6. EFFECT OF GRAFTING DIFFERENT PUMPKIN STOCKS ON GROWTH OF NETTED MELON%不同南瓜砧木品种对网纹甜瓜生长发育的影响

    Institute of Scientific and Technical Information of China (English)

    陈幼源; 陈绯翔

    2002-01-01

    用六个常用的南瓜(Cucurbita moschata Duch.)砧木品种嫁接网纹甜瓜(Cucumis melo L.varreticulatusNand.),试验结果表明:网纹甜瓜嫁接后,可大大降低枯萎病(Fusarium oxysporum f.sp.Cucumerinum)、疫霉病(Phytophthoraa drechsleri Tucker)等土传病害及蔓枯病[Mycosphaerlla melonis(Pass.)Chiuet Walker]的发生率,但各砧木品种的表现不同,综合各砧木品种的性状表现,其中以刚力、铁かぶと和"ジセスト"较适宜作为网纹甜瓜的嫁接砧木;黑籽南瓜(C.fieifolia Bouche)、白菊座不宜作为网纹甜瓜的砧木品种.网纹甜瓜嫁接后尽管能明显降低几种主要病害的发生率,但其不利的影响也较明显,受影响的主要性状有果实可溶性固形物、外观、果肉的质地、风味等.

  7. In vivo metabolism of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) in young whole pumpkin plant.

    Science.gov (United States)

    Sun, Jianteng; Liu, Jiyan; Yu, Miao; Wang, Chang; Sun, Yuzhen; Zhang, Aiqian; Wang, Thanh; Lei, Zhen; Jiang, Guibin

    2013-04-16

    Polybrominated diphenyl ethers (PBDEs) are widely distributed persistent organic pollutants. In vitro and in vivo research using various animal models have shown that PBDEs might be transformed to hydroxylated PBDEs, but there are few studies on in vivo metabolism of PBDEs by intact whole plants. In this research, pumpkin plants (Cucurbita maxima × C. moschata) were hydroponically exposed to 2,2',4,4'-tetrabromodiphenyl ether (BDE-47). A debromination product (BDE-28) and four hydroxylated metabolites (5-OH-BDE-47, 6-OH-BDE-47, 4'-OH-BDE-49, and 4-OH-BDE-42) were detected in different parts of the whole plant. In addition, 4-methoxylated-2,2',3,4'-tetraBDE (4-MeO-BDE-42) was observed as a methoxylation product. Root exudates in solution were found to play an important role in metabolizing BDE-47 to a specific OH-PBDE: 4'-OH-BDE-49. BDE-28 was found to translocate more easily and accumulate in shoots than BDE-47 due to the lower hydrophobicity and molecular weight. The concentration ratio between metabolites and parent compound BDE-47 was lower for OH-PBDEs than that for both BDE-28 and 4-MeO-BDE-42. The metabolism pathway of BDE-47 in young whole plants was proposed in this study.

  8. Molecular Markers Linked to the Dwarf Gene in Squash%与南瓜矮生基因连锁的分子标记

    Institute of Scientific and Technical Information of China (English)

    李云龙; 李海真; 崔崇士; 张海英; 宫国义

    2007-01-01

    以中国南瓜(Cucurbita moschata Duch)矮生突变体(矮10)为供体亲本,以印度蔓生南瓜(C.maxima Duch)(蒙日南瓜)为轮回亲本,经6代回交和2代自交选育出S1~S9共9个南瓜矮生近等基因纯合株系,其中S2株系再与蒙日南瓜杂交获得F2分离群体共306株.利用RAPD技术,采用近等基因系(near isogenic lines,NILs)分析法,结果表明,在640条随机引物中,发现RAPD标记S1225-548与矮生单基因D呈现连锁关系,遗传距离为2.29 cM.RAPD标记成功转化成更为稳定的SCAR标记SCAR3-398.

  9. A molecular model for diacylglycerol acyltransferase from Mortierella ramanniana var. angulispora.

    Science.gov (United States)

    Mishra, Sanjay; Dwivedi, Surya Prakash; Dwivedi, Neeraja; Kumar, Ajay; Rawat, Anil; Kamisaka, Yasushi

    2009-06-28

    Acyl CoA diacylglycerol acyltransferase (DGAT, EC 2.3.120) is recognized as a key player of cellular diacylglycerol metabolism. It catalyzes the terminal, yet the committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl CoA as substrates. The protein sequence of diacylglycerol acyltransferse (DGAT) Type 2B in Moretierella ramanniana var. angulispora (Protein_ID = AAK84180.1) was retrieved from GenBank. However, a structure is not yet available for this sequence. The 3D structure of DGAT Type 2B was modeled using a template structure (PDB ID: 1K30) obtained from Protein databank (PDB) identified by searching with position specific iterative BLAST (PSI-BLAST). The template (PDB ID: 1K30) describes the structure of DGAT from Cucurbita moschata. Modeling was performed using Modeller 9v2 and protein model is hence generated. The DGAT type 2B protein model was subsequently docked with six inhibitors (sphingosine; trifluoroperazine; phosphatidic acid; lysophospatidylserine; KCl; 1, 2-diolein) using AutoDock (a molecular docking program). The binding of inhibitors to the protein model of DGAT type 2B is discussed.

  10. Characterization of the major integral protein of vacuolar membrane.

    Science.gov (United States)

    Maeshima, M

    1992-04-01

    The vacuolar membrane of radish (Raphanus sativus) taproot contained a large quantity of a protein of 23 kilodaltons that accounted for more than 25% of the total membrane proteins. The protein, tentatively named VM 23, was purified and characterized. VM 23 tends to aggregate at high temperature even in the presence of 1% sodium dodecyl sulfate. The apparent molecular size of VM 23 was estimated to be about 400 kilodaltons by polyacrylamide gel electrophoresis in the presence of 0.1% Triton X-100. VM 23 was partially extracted from the vacuolar membranes with chloroform:methanol, indicating its high hydrophobicity. The hydrophobic carboxyl modifier N,N'-dicyclohexylcarbodiimide bound covalently to VM 23. The results suggest that VM 23 may act as a secondary transport system coupled with the proton transport. The antibody against radish VM 23 reacted with the major proteins in the vacuolar membranes of mung bean (Vigna radiata) and castor bean (Ricinus communis) hypocotyls and pumpkin (Cucurbita moschata) epicotyl, but not with that of sugar beet (Beta vulgaris) taproot. VM 23 comigrated with vacuolar H(+)-pyrophosphatase on sucrose density gradient centrifugation after sonication of membranes, indicating that it is associated with the vacuolar membrane.

  11. Glutamate dehydrogenase from pumpkin cotyledons: characterization and isoenzymes.

    Science.gov (United States)

    Chou, K H; Splittstoesser, W E

    1972-04-01

    Glutamate dehydrogenase from pumpkin (Cucurbita moschata Pior. cultivar Dickinson Field) cotyledons was found in both soluble and particulate fractions with the bulk of the activity in the soluble fraction. Both enzymes used NAD(H) and NADP(H) but NAD(H) was favored. The enzymes were classified as glutamate-NAD oxidoreductase, deaminating (EC 1.4.1.3). Both enzymes were heat stable, had a pH optimum for reductive amination of 8.0, and were inhibited by high concentrations of NH(4) (+) or alpha-ketoglutarate. The soluble enzyme was more sensitive to NH(4) (+) inhibition and was activated by metal ions after ammonium sulfate fractionation while the solubilized particulate enzyme was not. Inhibition by ethylenediaminetetraacetate was restored by several divalent ions and inhibition by p-hydroxymercuribenzoate was reversed by glutathione. Particulate glutamate dehydrogenase showed a greater activity with NADP. The molecular weights of the enzymes are 250,000. Separation of the enzymes by disc gel electrophoresis showed that during germination the soluble isoenzymes increased from 1 to 7 in number, while only one particulate isoenzyme was found at any time. This particulate isoenzyme was identical with one of the soluble isoenzymes. A number of methods indicated that the soluble isoenzymes were not simply removed from the particulate fraction and that true isoenzymes were found.

  12. Antilisterial effects of ethanolic extracts of some edible Thai plants on refrigerated cooked pork

    Directory of Open Access Journals (Sweden)

    Siriporn Stonsaovapak

    2010-12-01

    Full Text Available Listeria monocytogenes is a major foodborne pathogen responsible for the disease listeriosis.Effective methods for reducing L. monocytogenes in foods would reduce the likelihood of foodborneoutbreaks of listeriosis and decrease economic losses to the food industry. Crude ethanolic extracts from 50 edible Thai plants were screened for inhibitory effects on isolated strains and type strains of L.monocytogenes by the well assay technique. Ethanolic extracts of Micromelum minutum, Artocarpus heterophyllus, Piper retrofractum and Cucurbita moschata, which showed listerial growth inhibition,were applied to cooked pork to determine their antimicrobial activities against L. monocytogenes. Pork was cooked to an internal temperature of 85C, allowed to cool to 8C and then treated by surface application with the plant extracts. Low (102 cfu g-1 or high (105 cfu g-1 population of L.monocytogenes were applied and samples were stored at 4C for up to 7 days. M. minutum and A.heterophyllus extracts were most effective in inhibiting the growth of the pathogen. These results suggested that some edible Thai plant extracts might be useful as antimicrobials in cooked, ready-to-eatpork.

  13. Extracción y caracterización de aceite de semillas de zapallo

    Directory of Open Access Journals (Sweden)

    Valdés Restrepo Magda Piedad

    2009-09-01

    Full Text Available Se seleccionaron 79 introducciones de zapallo (Cucurbita moschata Duch teniendo en cuenta el contenido de extracto etéreo (EE de las semillas. El EE fue estable física y químicamente, con propiedades organolépticas óptimas de aceite comestible, no presentó características de rancidez; aceite semisecante (122.90 mg/g de KOH.; índice de acidez 3.25 mg/g de KOH; la prueba presuntiva de yodo supone presencia de polinsaturación y sin formación de cristales. La composición de ácidos grasos mostró: palmítico C16:0 (25.11 – 36.94%; esteárico C18:0 (10.79 – 13.37%; linoleíco C18:2 (48.23 – 62.41%; linolénico C18:3 (0.66% y araquídico C20:0 (0.53 – 0.78%. El aceite de semilla de zapallo contiene 55.28% de ácidos grasos insaturados con una cantidad apreciable de linoleíco (55.11%. La torta de semilla presentó proteína (51.11 ± 0.95% y energía (4604. 66 ± 134.08 kcal/kg

  14. Crop candidates for the bioregenerative life support systems in China

    Science.gov (United States)

    Chunxiao, Xu; Hong, Liu

    The use of plants for life support applications in space is appealing because of the multiple life support functions by the plants. Research on crops that were grown in the life support system to provide food and oxygen, remove carbon dioxide was begun from 1960. To select possible crops for research on the bioregenerative life support systems in China, criteria for the selection of potential crops were made, and selection of crops was carried out based on these criteria. The results showed that 14 crops including 4 food crops (wheat, rice, soybean and peanut) and 7 vegetables (Chinese cabbage, lettuce, radish, carrot, tomato, squash and pepper) won higher scores. Wheat ( Triticum aestivum L.), rice ( Oryza sativa L.), soybean ( Glycine max L.) and peanut ( Arachis hypogaea L.) are main food crops in China. Chinese cabbage ( Brassica campestris L. ssp. chinensis var. communis), lettuce ( Lactuca sativa L. var. longifolia Lam.), radish ( Raphanus sativus L.), carrot ( Daucus carota L. var. sativa DC.), tomato ( Lycopersicon escalentum L.), squash ( Cucurbita moschata Duch.) and pepper ( Capsicum frutescens L. var. longum Bailey) are 7 vegetables preferred by Chinese. Furthermore, coriander ( Coriandum sativum L.), welsh onion ( Allium fistulosum L. var. giganteum Makino) and garlic ( Allium sativum L.) were selected as condiments to improve the taste of space crew. To each crop species, several cultivars were selected for further research according to their agronomic characteristics.

  15. Extracción y caracterización de aceite de semillas de zapallo

    Directory of Open Access Journals (Sweden)

    Sanín Ortiz Grisales

    2009-07-01

    Full Text Available Se seleccionaron 79 introducciones de zapallo (Cucurbita moschata Duch teniendo en cuenta el contenido de extracto etéreo (EE de las semillas. El EE fue estable física y químicamente, con propiedades organolépticas óptimas de aceite comestible, no presentó características de rancidez; aceite semisecante (122.90 mg/g de KOH.; índice de acidez 3.25 mg/g de KOH; la prueba presuntiva de yodo supone presencia de polinsaturación y sin formación de cristales. La composición de ácidos grasos mostró: palmítico C16:0 (25.11 - 36.94%; esteárico C18:0 (10.79 - 13.37%; linoleíco C18:2 (48.23 - 62.41%; linolénico C18:3 (0.66% y araquídico C20:0 (0.53 - 0.78%. El aceite de semilla de zapallo contiene 55.28% de ácidos grasos insaturados con una cantidad apreciable de linoleíco (55.11%. La torta de semilla presentó proteína (51.11 ± 0.95% y energía (4604. 66 ± 134.08 kcal/kg.

  16. 南瓜籽活性成分研究及应用

    Institute of Scientific and Technical Information of China (English)

    宗玉丽; 李鑫; 付英梅; 郭玲玲

    2011-01-01

    @@ 南瓜(cucurbita moschata Duch.),别名番瓜、饭瓜等,是葫芦科一年生草本植物.南瓜的营养价值很高,除含有多种游离氨基酸、胡罗卜素、VB、VC、甘露醇等营养成分外,还含有多种功效成分,国内对南瓜的研究较多,对南瓜籽的研究起步较晚.南瓜籽为南瓜的成熟种子,其含有丰富的不饱和脂肪酸、维生素、生物黄酮、南瓜多糖及矿物质等,这些功效成分有着广泛而重要的生物学功能,具有较好的研究与应用价值.

  17. Complete nucleotide sequence of watermelon chlorotic stunt virus originating from Oman.

    Science.gov (United States)

    Khan, Akhtar J; Akhtar, Sohail; Briddon, Rob W; Ammara, Um; Al-Matrooshi, Abdulrahman M; Mansoor, Shahid

    2012-07-01

    Watermelon chlorotic stunt virus (WmCSV) is a bipartite begomovirus (genus Begomovirus, family Geminiviridae) that causes economic losses to cucurbits, particularly watermelon, across the Middle East and North Africa. Recently squash (Cucurbita moschata) grown in an experimental field in Oman was found to display symptoms such as leaf curling, yellowing and stunting, typical of a begomovirus infection. Sequence analysis of the virus isolated from squash showed 97.6-99.9% nucleotide sequence identity to previously described WmCSV isolates for the DNA A component and 93-98% identity for the DNA B component. Agrobacterium-mediated inoculation to Nicotiana benthamiana resulted in the development of symptoms fifteen days post inoculation. This is the first bipartite begomovirus identified in Oman. Overall the Oman isolate showed the highest levels of sequence identity to a WmCSV isolate originating from Iran, which was confirmed by phylogenetic analysis. This suggests that WmCSV present in Oman has been introduced from Iran. The significance of this finding is discussed.

  18. Dehydrodiconiferyl alcohol (DHCA) modulates the differentiation of Th17 and Th1 cells and suppresses experimental autoimmune encephalomyelitis.

    Science.gov (United States)

    Lee, Junghun; Choi, Jinyong; Lee, Wonwoo; Ko, Kyeongryang; Kim, Sunyoung

    2015-12-01

    Dehydrodiconiferyl alcohol (DHCA), originally isolated from the stems of Cucurbita moschata, has previously been shown to exhibit anti-adipogenic and anti-lipogenic effects in 3T3-L1 cells and primary mouse embryonic fibroblasts (MEFs) (Lee et al., 2012). Here, we investigated whether synthetic DHCA could suppress the CD4 T helper 17 (Th17)-mediated production of the interleukin (IL)-17 protein. The results from RT-qPCR suggest that DHCA-mediated down-regulation of IL-17 occurred at the transcriptional level by suppressing the expression of RAR-related orphan receptor (ROR)γt, the master transcription factor involved in the differentiation of Th17 cells. Furthermore, such inhibition was mediated by the suppression of NF-κB activity. DHCA also inhibited the Th1-mediated production of interferon (IFN) γ by controlling the expression of a key transcription factor known to regulate the production of this cytokine, T-bet. In the mouse experimental autoimmune encephalomyelitis (EAE) model, DHCA showed significant therapeutic effects by inhibiting the infiltration of immune cells into the spinal cords, decreasing the differentiation of pathogenic Th17 and Th1 cells, suppressing the expression of various pro-inflammatory cytokines, and eventually ameliorating the clinical symptoms of EAE mice. Taken together, our data indicate that DHCA may be a potential candidate as an agent for the control of Th17 and Th1-mediated inflammatory diseases.

  19. Functional group diversity of bee pollinators increases crop yield.

    Science.gov (United States)

    Hoehn, Patrick; Tscharntke, Teja; Tylianakis, Jason M; Steffan-Dewenter, Ingolf

    2008-10-01

    Niche complementarity is a commonly invoked mechanism underlying the positive relationship between biodiversity and ecosystem functioning, but little empirical evidence exists for complementarity among pollinator species. This study related differences in three functional traits of pollinating bees (flower height preference, daily time of flower visitation and within-flower behaviour) to the seed set of the obligate cross-pollinated pumpkin Cucurbita moschata Duch. ex Poir. across a land-use intensity gradient from tropical rainforest and agroforests to grassland in Indonesia. Bee richness and abundance changed with habitat variables and we used this natural variation to test whether complementary resource use by the diverse pollinator community enhanced final yield. We found that pollinator diversity, but not abundance, was positively related to seed set of pumpkins. Bees showed species-specific spatial and temporal variation in flower visitation traits and within-flower behaviour, allowing for classification into functional guilds. Diversity of functional groups explained even more of the variance in seed set (r2=45%) than did species richness (r2=32%) highlighting the role of functional complementarity. Even though we do not provide experimental, but rather correlative evidence, we can link spatial and temporal complementarity in highly diverse pollinator communities to pollination success in the field, leading to enhanced crop yield without any managed honeybees.

  20. 组培条件下中国南瓜杂交种耐盐材料的筛选%SELECTION OF SALT-TOLERANT MATERIALS OF PUMPKIN HYBRIDS in vitro

    Institute of Scientific and Technical Information of China (English)

    周俊国; 朱月林; 刘正鲁; 王建国

    2007-01-01

    在组培条件下以69份中国南瓜(Cucurbita moschata Duch.)杂交种和4份商品种为试验材料,研究中国南瓜杂交种的耐盐状况,确定筛选耐盐材料的适当NaCl浓度.结果表明,中国南瓜杂交种在不同NaCl浓度处理下存在明显的耐盐性差异,69份杂交种在120mmol/L NaCl浓度处理下的盐胁迫指数介于29.94~100之间,呈正态分布,有6份杂交种表现出较高的耐盐性,其中360-3×112-2杂交种最耐盐.认为在组培条件下采用120mmol/LNaCl浓度处理中国南瓜幼苗是一种有效筛选耐盐材料的方法.