Juan J. Loor
Full Text Available Dairy cows undergo tremendous metabolic and physiological adaptations around parturition to support lactation. The liver is central to many of these processes, including gluconeogenesis and metabolism of fatty acids mobilized from adipose tis- sue. Fat accumulation may impair normal functions of the liver and increase ketogenesis, which in turn may predispose cows to other metabolic abnormalities. Several aspects of dietary management and body condition may alter these adaptations, affect dry matter intake, and increase or decrease susceptibility to periparturient health problems. Overfeeding energy dur- ing the dry period is a prominent risk factor. Considerable progress has been made in recent years in describing the adap- tive changes in the liver and other organs in normal and abnormal states, but this knowledge has not yet identified unequiv- ocally the key steps that might be compromised during development of metabolic disorders. The potential role of signaling compounds, such as the inflammatory cytokines released in response to environmental stressors, infectious challenge, and oxidative stress, in the pathogenesis of periparturient disease is under investigation. New techniques such as functional genomics, using cDNA or oligonucleotide microarrays, as well as proteomics and metabolomics, provide additional high- throughput tools to determine the effects of nutrition, management, or stressors on tissue function in development of dis- ease. Integrative approaches should be fruitful in unraveling the complex interactions of metabolism, immune activation, stress physiology, and endocrinology that likely underlie development of periparturient disease.
Williams, JB; Tieleman, BI; Williams, Joseph B.
We call into question the idea that birds have not evolved unique physiological adaptations to desert environments. The rate at which desert larks metabolize energy is lower than in mesic species within the same family, and this lower rate of living translates into a lower overall energy requirement
Wang, Qiangjun; Zhao, Xiaowei; Zhang, Zijun; Zhao, Huiling; Huang, Dongwei; Cheng, Guanglong; Yang, Yongxin
Lactation performance of dairy cattle is susceptible to heat stress. The liver is one of the most crucial organs affected by high temperature in dairy cows. However, the physiological adaption by the liver to hot summer conditions has not been well elucidated in lactating dairy cows. In the present study, proteomic analysis of the liver in dairy cows in spring and hot summer was performed using a label-free method. In total, 127 differentially expressed proteins were identified; most of the upregulated proteins were involved in protein metabolic processes and responses to stimuli, whereas most of the downregulated proteins were related to oxidation-reduction. Pathway analysis indicated that 3 upregulated heat stress proteins (HSP90α, HSP90β, and endoplasmin) were enriched in the NOD-like receptor signaling pathway, whereas several downregulated NADH dehydrogenase proteins were involved in the oxidative phosphorylation pathway. The protein-protein interaction network indicated that several upregulated HSPs (HSP90α, HSP90β, and GRP78) were involved in more interactions than other proteins and were thus considered as central hub nodes. Our findings provide novel insights into the physiological adaption of liver function in lactating dairy cows to natural high temperature. Copyright © 2017. Published by Elsevier Ltd.
Moyes, Kasey; Friggens, Nic; Ingvartsen, Klaus Lønne
Thirty Danish Holstein cows were used to determine how cows in early lactation adapt to changes in protein to starch supply in order to manipulate metabolism to combat physiological imbalance. During weeks 4 through 6 of lactation, 10 cows were fed either a high protein to starch ratio (high) diet...... for the low, control and high diets, respectively. Besides milk urea nitrogen, no other production or metabolic parameters were affected by treatment. In conclusion, manipulation of dietary protein to starch is not a potential strategy to combat physiological imbalance during early lactation...
Thatcher, W W; Santos, J E P; Silvestre, F T; Kim, I H; Staples, C R
Increasing reproductive performance of post-partum lactating dairy cows is a multi-factorial challenge involving disciplines of production medicine, nutrition, physiology and herd management. Systems of programmed timed insemination have been fine-tuned to achieve pregnancy per artificial inseminations (AI) approximating 45%. Systems have optimized follicle development, integrated follicle development with timing of induced corpus luteum regression and fine-tuned sequential timing of induced ovulation and AI. Use of programmes for insemination have identified occurrence of anovulatory ovarian status, body condition, uterine health and seasonal summer stress as factors contributing to reduced herd fertility. Furthermore, programmes of timed insemination provide a platform to evaluate efficacy of nutritional and herd health systems targeted to the transition and post-partum periods. The homeorhetic periparturient period, as cows deal with decreases in dry matter intake, results in a negative energy balance and is associated with a period of immunosuppression. Cows that transition well will cycle earlier and have a greater risk of becoming pregnant earlier post-partum. The innate arms of the immune system (acute and adaptive) are suppressed during the periparturient period. Cows experiencing the sequential complex of disorders such as dystocia, puerperal metritis, metritis, endometritis and subclinical endometritis are subsequently less fertile. Targeted strategies of providing specific nutraceuticals that provide pro- and anti-inflammatory effects, such as polyunsaturated fatty acids (e.g., linoleic, eicosapentaenoic/docosahexaenoic, conjugated linoleic acid), sequential glycogenic and lipogenic enrichment of diets, and organic selenium appear to differentially regulate and improve the immune and reproductive systems to benefit an earlier restoration of ovarian activity and increased fertility. © 2010 Blackwell Verlag GmbH.
Derno, Michael; Otten, Winfried; Mielenz, Manfred; Nürnberg, Gerd
High ambient temperatures have severe adverse effects on biological functions of high-yielding dairy cows. The metabolic adaption to heat stress was examined in 14 German Holsteins transition cows assigned to two groups, one heat-stressed (HS) and one pair-fed (PF) at the level of HS. After 6 days of thermoneutrality and ad libitum feeding (P1), cows were challenged for 6 days (P2) by heat stress (temperature humidity index (THI) = 76) or thermoneutral pair-feeding in climatic chambers 3 weeks ante partum and again 3 weeks post-partum. On the sixth day of each period P1 or P2, oxidative metabolism was analyzed for 24 hours in open circuit respiration chambers. Water and feed intake, vital parameters and milk yield were recorded. Daily blood samples were analyzed for glucose, β-hydroxybutyric acid, non-esterified fatty acids, urea, creatinine, methyl histidine, adrenaline and noradrenaline. In general, heat stress caused marked effects on water homeorhesis with impairments of renal function and a strong adrenergic response accompanied with a prevalence of carbohydrate oxidation over fat catabolism. Heat-stressed cows extensively degraded tissue protein as reflected by the increase of plasma urea, creatinine and methyl histidine concentrations. However, the acute metabolic heat stress response in dry cows differed from early-lactating cows as the prepartal adipose tissue was not refractory to lipolytic, adrenergic stimuli, and the rate of amino acid oxidation was lower than in the postpartal stage. Together with the lower endogenous metabolic heat load, metabolic adaption in dry cows is indicative for a higher heat tolerance and the prioritization of the nutritional requirements of the fast-growing near-term fetus. These findings indicate that the development of future nutritional strategies for attenuating impairments of health and performance due to ambient heat requires the consideration of the physiological stage of dairy cows. PMID:25938406
Lamp, Ole; Derno, Michael; Otten, Winfried; Mielenz, Manfred; Nürnberg, Gerd; Kuhla, Björn
High ambient temperatures have severe adverse effects on biological functions of high-yielding dairy cows. The metabolic adaption to heat stress was examined in 14 German Holsteins transition cows assigned to two groups, one heat-stressed (HS) and one pair-fed (PF) at the level of HS. After 6 days of thermoneutrality and ad libitum feeding (P1), cows were challenged for 6 days (P2) by heat stress (temperature humidity index (THI) = 76) or thermoneutral pair-feeding in climatic chambers 3 weeks ante partum and again 3 weeks post-partum. On the sixth day of each period P1 or P2, oxidative metabolism was analyzed for 24 hours in open circuit respiration chambers. Water and feed intake, vital parameters and milk yield were recorded. Daily blood samples were analyzed for glucose, β-hydroxybutyric acid, non-esterified fatty acids, urea, creatinine, methyl histidine, adrenaline and noradrenaline. In general, heat stress caused marked effects on water homeorhesis with impairments of renal function and a strong adrenergic response accompanied with a prevalence of carbohydrate oxidation over fat catabolism. Heat-stressed cows extensively degraded tissue protein as reflected by the increase of plasma urea, creatinine and methyl histidine concentrations. However, the acute metabolic heat stress response in dry cows differed from early-lactating cows as the prepartal adipose tissue was not refractory to lipolytic, adrenergic stimuli, and the rate of amino acid oxidation was lower than in the postpartal stage. Together with the lower endogenous metabolic heat load, metabolic adaption in dry cows is indicative for a higher heat tolerance and the prioritization of the nutritional requirements of the fast-growing near-term fetus. These findings indicate that the development of future nutritional strategies for attenuating impairments of health and performance due to ambient heat requires the consideration of the physiological stage of dairy cows.
Full Text Available High ambient temperatures have severe adverse effects on biological functions of high-yielding dairy cows. The metabolic adaption to heat stress was examined in 14 German Holsteins transition cows assigned to two groups, one heat-stressed (HS and one pair-fed (PF at the level of HS. After 6 days of thermoneutrality and ad libitum feeding (P1, cows were challenged for 6 days (P2 by heat stress (temperature humidity index (THI = 76 or thermoneutral pair-feeding in climatic chambers 3 weeks ante partum and again 3 weeks post-partum. On the sixth day of each period P1 or P2, oxidative metabolism was analyzed for 24 hours in open circuit respiration chambers. Water and feed intake, vital parameters and milk yield were recorded. Daily blood samples were analyzed for glucose, β-hydroxybutyric acid, non-esterified fatty acids, urea, creatinine, methyl histidine, adrenaline and noradrenaline. In general, heat stress caused marked effects on water homeorhesis with impairments of renal function and a strong adrenergic response accompanied with a prevalence of carbohydrate oxidation over fat catabolism. Heat-stressed cows extensively degraded tissue protein as reflected by the increase of plasma urea, creatinine and methyl histidine concentrations. However, the acute metabolic heat stress response in dry cows differed from early-lactating cows as the prepartal adipose tissue was not refractory to lipolytic, adrenergic stimuli, and the rate of amino acid oxidation was lower than in the postpartal stage. Together with the lower endogenous metabolic heat load, metabolic adaption in dry cows is indicative for a higher heat tolerance and the prioritization of the nutritional requirements of the fast-growing near-term fetus. These findings indicate that the development of future nutritional strategies for attenuating impairments of health and performance due to ambient heat requires the consideration of the physiological stage of dairy cows.
Palacio, S; Bergeron, R; Lachance, S; Vasseur, E
Access to pasture has advantages for cows such as reduced lameness and improved udder health, but also may expose cows to stressors such as extreme heat. The objective of this study was to understand how portable shade affected physiological and behavioral responses of pastured dairy cows in a Canadian summer. Over 8wk, a total of 24 lactating Holstein cows were separated into 2 treatments, one with access to shade and a control without access to shade. The cows were pastured in groups of 4, with 3 field sections per treatment. Instantaneous scan sampling of behaviors (drinking, lying, grazing, other) performed in the shade or not were recorded every 5min for 3h/d during the hottest part of the day (peak hours: 1130-1530h) 3d/wk. Ambient temperature, humidity, and vaginal temperature were recorded at 10-min intervals. Daily milk production was also recorded. Differences between treatments by week were analyzed using the generalized linear mixed model with group as random effect and treatment as fixed effect. Cows with shade access were observed at the water trough up to 6.42 times less and lying down up to 1.75 times more. Cows with shade access grazed up to 1.5 times more but only when the temperature-humidity index was above their comfort threshold (≥72) during the hottest part of the day (wk 2). Cows sought shade when it was made available, but spent less than half of their time observed (%) in the shade (40.8±4.67) with the exception of wk 2 when most of the time was spent under the shade (74.3±4.77). Daily lying time was highest during peak hours for cows with shade access. However, no overall difference in total lying time between the 2 treatments was observed. No differences were found in vaginal temperature or milk production between treatments with the exception of wk 1 for daily milk production, which was higher for cows in the control treatment. In conclusion, cows sought shade when it was provided at pasture, whereas cows without access to shade
Full Text Available Stress factors are so numerous and so diverse in their strength and duration that the consequences on animal welfare can be quite varied. The first important distinction concerns the characterization of acute and chronic stress conditions. Acute stress is a short-lived negative situation that allows a quick and quite complete recovery of the physiological balance (adaptation, while chronic stress is a long lasting condition from which the subject cannot fully recover (maladaptation. In the latter case, the direct effects of the stress factors (heat, low energy, anxiety, suffering etc., as well as the indirect ones (changes occurring at endocrinological, immune system or function level can be responsible for pre-pathological or pathological consequences which reduce animal welfare. To evaluate the possible chronic stress conditions in single animals or on a farm (in particular a farm of dairy cows, some parameters of the direct or indirect effects can be utilised. They are physiological (mainly hormone changes: cortisol, β-endorphin, behavioural (depression, biochemical (metabolites, acute phase proteins, glycated proteins etc., as well as performance parameters (growing rate, milk yield, fertility, etc.. Special attention has been paid to the interpretation of cortisol levels and to its changes after an ACTH challenge. Despite fervent efforts, well established and accepted indices of chronic stress (distress are currently lacking; but without this objective evaluation, the assessment of animal welfare and, therefore, the optimization of the livestock production, could prove more difficult.
Gallardo, M. R.; Valtorta, S. E.; Leva, P. E.; Gaggiotti, M. C.; Conti, G. A.; Gregoret, R. F.
The objective of this trial was to evaluate the effects of diet and cooling in the holding pen before milking on rectal temperature, respiration rate and milk production and composition. Fifty-eight lactating Holstein cows were used in a factorial split-plot design, at Rafaela Experimental Station from 12 January to 3 March 2003. The treatments were combinations of two diets: control (CD) and balanced (BD) with two levels of cooling before milking: none (NSF) and a sprinkler and fans (SF). Forage:concentrate ratios for CD and BD were 81:19 and 68:32, respectively. Cows were milked twice daily. Milk production was recorded daily, and milk composition (fat, protein, lactose and urea) was analysed twice a week. The physiological data were recorded once a week, before the cattle entered the holding pen and after milking, in the afternoon. Average maximum weekly temperature humidity index was 75.4 and ranged from 61.4 to 83. There were highly significant effects of cooling on physiological responses. Milk production was affected by diet and cooling, with no interaction; the highest and lowest production of milk was 22.42 and 20.07 l/cow per day, for BD+SF and CD+NSF, respectively. Protein was affected by diet, and was higher for BD (3.17 vs. 3.08%). There were interaction effects on milk fat at the 8% level, the highest concentration being 3.65% for BD+NFS. It was concluded that under grazing conditions, cooling by sprinkler and fans before milking improves the comfort of dairy cows, and that the effects on milk production and composition are enhanced when diets are specially formulated for heat-stress periods.
Legrand, A; Schütz, K E; Tucker, C B
Water is commonly used to cool cattle in summer either at milking or over the feed bunk, but little research has examined how dairy cows voluntarily use water separate from these locations. The objectives were to describe how and when dairy cattle voluntarily used an overhead water source separate from other resources, such as feed, and how use of this water affected behavioral and physiological indicators of heat stress. Half of the 24 nonlactating cattle tested had access to a "cow shower" composed of 2 shower heads activated by a pressure-sensitive floor. All animals were individually housed to prevent competition for access to the shower. Over 5 d in summer (air temperature=25.3±3.3°C, mean ± standard deviation), cattle spent 3.0±2.1 h/24h in the shower, but considerable variability existed between animals (individual daily values ranged from 0.0 to 8.2 h/24h). A portion of this variation can be explained by weather; shower use increased by 0.3h for every 1°C increase in ambient temperature. Cows preferentially used the shower during the daytime, with 89±12% of the time spent in the shower between 1000 and 1900 h. Respiration rate and skin temperature did not differ between treatments [53 vs. 61 breaths/min and 35.0 vs. 35.4°C in shower and control cows, respectively; standard error of the difference (SED)=5.6 breaths/min and 0.49°C]. In contrast, body temperature of cows provided with a shower was 0.2°C lower than control cows in the evening (i.e., 1800 to 2100h; SED=0.11°C). Cows with access to a shower spent half as much time near the water trough than control animals, and this pattern became more pronounced as the temperature-humidity index increased. In addition, cattle showed other behavioral changes to increasing heat load; they spent less time lying when heat load index increased, but the time spent lying, feeding, and standing without feeding did not differ between treatments. Cows had higher respiration rate, skin temperature, and body
Baffy, György; Loscalzo, Joseph
Living organisms constantly interact with their surroundings and sustain internal stability against perturbations. This dynamic process follows three fundamental strategies (restore, explore, and abandon) articulated in historical concepts of physiological adaptation such as homeostasis, allostasis, and the general adaptation syndrome. These strategies correspond to elementary forms of behavior (ordered, chaotic, and static) in complex adaptive systems and invite a network-based analysis of the operational characteristics, allowing us to propose an integrated framework of physiological adaptation from a complex network perspective. Applicability of this concept is illustrated by analyzing molecular and cellular mechanisms of adaptation in response to the pervasive challenge of obesity, a chronic condition resulting from sustained nutrient excess that prompts chaotic exploration for system stability associated with tradeoffs and a risk of adverse outcomes such as diabetes, cardiovascular disease, and cancer. Deconstruction of this complexity holds the promise of gaining novel insights into physiological adaptation in health and disease. Published by Elsevier Inc.
Rafael Nogueira Furtado
Full Text Available The aim of the present study was to evaluate the effect of substituting Tifton-85 grass hay with different quantities of xiquexique (Pilosocereus gounellei (0, 12, 24, and 36% on the nutrient intake and physiological responses of lactating dairy cows. Eight multiparous crossbred cows at approximately 100 days in milk, with an average milk yield of 15 kg of milk per day-1, and an average body weight (BW of 465.20 ± 39.37 kg, were distributed in a 4 × 4 double Latin square design. Each experimental period lasted 16 days, consisting of 10 days for adaptation and 6 days for data collection, giving a total of 64 experimental days. The roughage: concentrate ratio was 60:40, on a dry matter (DM basis. The DM intake, expressed in kg day-1, was affected quadratically by the levels of xiquexique in the diets. The intakes of DM, expressed in % BW and g kg-0.75, and neutral detergent fiber (NDF in the three units analyzed (kg day-1, % BW, and g kg-0.75, as well as the intakes of organic matter (OM, crude protein (CP, ether extract (EE, and total carbohydrates (TC, expressed in kg/day-1, decreased linearly with the levels of xiquexique in the diet. Milk yield (kg day-1 was reduced by the addition of xiquexique into the diet, but feed efficiency was not influenced. Water intake from feed (WIFeed, expressed in kg day-1 and % BW, incresed linearly with increasing levels of xiquexique in the total diet, while voluntary water intake, expressed in kg day-1 and % BW (WIVoluntary, decreased linearly. The total water intake (WITotal was not affected by experimental treatments. Participation of WIVoluntary in the WITotal linearly reduced with Xiquexique levels in the diet. The respiratory rate and surface temperature during both periods of the day (morning and afternoon, and rectal temperature during the morning were not influenced by the levels of xiquexique in the diet. Therefore, xiquexique can be utilized in the feeding of medium and high producing dairy cows
R.T. Mankowski (Robert T.); S.D. Anton (Stephen D.); T.W. Buford (Thomas W.); C. Leeuwenburgh (Christiaan)
textabstractIntroduction Adaptive responses to exercise training (ET) are crucial in maintaining physiologic homeostasis and health span. Exercise-induced aerobic bioenergetic reactions in the mitochondria and cytosol increase production of reactive oxygen species, where excess of reactive oxygen
Enemark, J M; Frandsen, A M; Thilsing-Hansen, T
The objective of the present study was to monitor serum and urine biochemical changes in dairy cows during and after oral administration of a synthetic sodium aluminium-silicate (zeolite A). A prospective longitudinal study involving four non-pregnant and non-lactating cows was chosen. Cows were......), while cows in the experimental group were fed the basic diet and supplemented with 1 kg zeolite pellets once daily. During the third week (period 3) both groups were fed the basic ration only and observed for any persistent effects after zeolite withdraw. Daily sampling included blood and urine....... Selected physiological parameters were compared between groups during period 2 and 3, whereas mean values from period 1, 2 and 3 were compared within the groups. Zeolite supplementation revealed a significant influence on calcium homeostasis. A slight decrease in serum Ca and in renal excretion of calcium...
Angela Maria de Vasconcelos
Full Text Available Aiming to evaluate the lactation performance and adaptability of confined dairy cows fed diets containing soybean and its by-products, this study used 12 Holsteincows with initial production of 30 kg milk-1 day-1 day-1 in feedlot system distributed in a 4 x 4 Latin square design. The experimental period lasted 84 days. The dry matter intake (DMI and meteorological variables were recorded daily. Milk production was measured from the 15th to the 21st day, with milk analysis twice in each period, and physiological variables collected on the 15, 17th and 21st days of each experimental period. The thermal comfort indices and rectal temperature were considered normal, however the respiratory frequency and heart rate were different between the periods. Total milk production and percentage of crude protein were not affected. The thermal environment had influence on the CMS and on the percentage of milk fat in warmer periods, but the mechanism of heat dissipation was efficient for the animals to maintain homeothermy without affecting milk production.
Prinzell, Lawrence J.; Pope, Alan T.; Freeman, Frederick G.
Adaptive automation has been proposed as a solution to current problems of human-automation interaction. Past research has shown the potential of this advanced form of automation to enhance pilot engagement and lower cognitive workload. However, there have been concerns voiced regarding issues, such as automation surprises, associated with the use of adaptive automation. This study examined the use of psychophysiological self-regulation training with adaptive automation that may help pilots deal with these problems through the enhancement of cognitive resource management skills. Eighteen participants were assigned to 3 groups (self-regulation training, false feedback, and control) and performed resource management, monitoring, and tracking tasks from the Multiple Attribute Task Battery. The tracking task was cycled between 3 levels of task difficulty (automatic, adaptive aiding, manual) on the basis of the electroencephalogram-derived engagement index. The other two tasks remained in automatic mode that had a single automation failure. Those participants who had received self-regulation training performed significantly better and reported lower National Aeronautics and Space Administration Task Load Index scores than participants in the false feedback and control groups. The theoretical and practical implications of these results for adaptive automation are discussed.
Weaver, Samantha R.; Prichard, Allan S.; Maerz, Noah L.; Prichard, Austin P.; Endres, Elizabeth L.; Hernández-Castellano, Lorenzo E.; Akins, Matthew S.; Bruckmaier, Rupert M.
Serotonin is known to regulate energy and calcium homeostasis in several mammalian species. The objective of this study was to determine if pre-partum infusions of 5-hydroxytryptophan (5-HTP), the immediate precursor to serotonin synthesis, could modulate energy homeostasis at the level of the hepatocyte in post-partum Holstein and Jersey dairy cows. Twelve multiparous Holstein cows and twelve multiparous Jersey cows were intravenously infused daily for approximately 7 d pre-partum with either saline or 1 mg/kg bodyweight of 5-HTP. Blood was collected for 14 d post-partum and on d30 post-partum. Liver biopsies were taken on d1 and d7 post-partum. There were no changes in the circulating concentrations of glucose, insulin, glucagon, non-esterified fatty acids, or urea nitrogen in response to treatment, although there were decreased beta-hydroxybutyrate concentrations with 5-HTP treatment around d6 to d10 post-partum, particularly in Jersey cows. Cows infused with 5-HTP had increased hepatic serotonin content and increased mRNA expression of the serotonin 2B receptor on d1 and d7 post-partum. Minimal changes were seen in the hepatic mRNA expression of various gluconeogenic enzymes. There were no changes in the mRNA expression profile of cell-cycle progression marker cyclin-dependent kinase 4 or apoptotic marker caspase 3, although proliferating cell nuclear antigen expression tended to be increased in Holstein cows infused with 5-HTP on d1 post-partum. Immunofluorescence assays showed an increased number of CASP3- and Ki67-positive cells in Holstein cows infused with 5-HTP on d1 post-partum. Given the elevated hepatic serotonin content and increased mRNA abundance of 5HTR2B, 5-HTP infusions may be stimulating an autocrine-paracrine adaptation to lactation in the Holstein cow liver. PMID:28922379
Samantha R Weaver
Full Text Available Serotonin is known to regulate energy and calcium homeostasis in several mammalian species. The objective of this study was to determine if pre-partum infusions of 5-hydroxytryptophan (5-HTP, the immediate precursor to serotonin synthesis, could modulate energy homeostasis at the level of the hepatocyte in post-partum Holstein and Jersey dairy cows. Twelve multiparous Holstein cows and twelve multiparous Jersey cows were intravenously infused daily for approximately 7 d pre-partum with either saline or 1 mg/kg bodyweight of 5-HTP. Blood was collected for 14 d post-partum and on d30 post-partum. Liver biopsies were taken on d1 and d7 post-partum. There were no changes in the circulating concentrations of glucose, insulin, glucagon, non-esterified fatty acids, or urea nitrogen in response to treatment, although there were decreased beta-hydroxybutyrate concentrations with 5-HTP treatment around d6 to d10 post-partum, particularly in Jersey cows. Cows infused with 5-HTP had increased hepatic serotonin content and increased mRNA expression of the serotonin 2B receptor on d1 and d7 post-partum. Minimal changes were seen in the hepatic mRNA expression of various gluconeogenic enzymes. There were no changes in the mRNA expression profile of cell-cycle progression marker cyclin-dependent kinase 4 or apoptotic marker caspase 3, although proliferating cell nuclear antigen expression tended to be increased in Holstein cows infused with 5-HTP on d1 post-partum. Immunofluorescence assays showed an increased number of CASP3- and Ki67-positive cells in Holstein cows infused with 5-HTP on d1 post-partum. Given the elevated hepatic serotonin content and increased mRNA abundance of 5HTR2B, 5-HTP infusions may be stimulating an autocrine-paracrine adaptation to lactation in the Holstein cow liver.
Martín-Tereso López, J.
Dairy cows suffer from hypocalcaemia in the days around calving, which may result in a condition generally known as milk fever. Calcium metabolism sharply shifts at the start of lactation, because Ca needs suddenly become much greater than at the end of gestation. Calcium metabolism is able to adapt
Kamphuis, C.; Frank, E.; Burke, J.; Verkerk, G.A.; Jago, J.
The hypothesis was that sensors currently available on farm that monitor behavioral and physiological characteristics have potential for the detection of lameness in dairy cows. This was tested by applying additive logistic regression to variables derived from sensor data. Data were collected
Baffy, Gyorgy; Loscalzo, Joseph
Living organisms constantly interact with their surroundings and sustain internal stability against perturbations. This dynamic process follows three fundamental strategies (restore, explore, and abandon) articulated in historical concepts of physiological adaptation such as homeostasis, allostasis, and the general adaptation syndrome. These strategies correspond to elementary forms of behavior (ordered, chaotic, and static) in complex adaptive systems and invite a network-based analysis of t...
Barragan Hernandez, Wilson Andres; Cajas-Giron, Yasmin Socorro; Mahecha-Ledesma, Lilliana
Changes in physiological and metabolic parameters were assessed as indicators of caloric stress of cows under grazing were investigated. The study was developed at the Centro de Investigacion Corpoica Turipana, Region Caribe, Cerete, Colombia, during the years 2011-2012. Temperature (T) and relative humidity (H), and in animals: rectal temperature (RT), skin temperature (TP), respiratory rate (RF) and acid-base status were determined. The variables were measured in the morning (6:00 h) and in the afternoon (13:00 h). Effect of treatment on environmental temperature was found with 7 and 6% less temperature in p-Arbur-Arbor and p-Arbor, respectively, compared with the grass treatment. There was an effect of time (p [es
Geburt, Katrin; Friedrich, Morten; Piechotta, Marion; Gauly, Matthias; König von Borstel, Uta
The objectives of the present study were to evaluate the suitability of potential biomarkers for maternal ability in cattle, and in addition to test the hypothesis that dairy cows have a less pronounced motherliness than beef cows. Therefore, maternal behavior of 20 Simmental beef-type (S) and 20 German Black Pied (dairy-type) Cattle (BP) was assessed on the 2nd and again on the 3rd day of the calf's life. Measurements included the frequency of interactions between cow and calf, the cow's willingness to defend her calf, the overall maternal behavior, saliva cortisol, saliva oxytocin, heart rate, and thermal images of the eye (ET). Mixed model analysis revealed that BP had significantly (Pcows. Simmental (beef) cows showed more defensive behavior (3.5±0.2 vs. 2.7±0.2 scores), but fewer total interactions between cow and calf (8.1±1.4 vs. 13.8±1.4), compared to BP (dairy). However, with the exception of heart rate and overall maternal behavior, breed differences tended to diminish from the 2nd to the 3rd day of the calf's life. Repeatabilities ranged from 9±23% (ET) to 77±7% (maternal behavior measured on a visual analogue scale), and correlations between physiological parameters and behavior differed between breeds and were generally at a low level. In conclusion, beef cows do not seem to be per se more maternal compared to dairy cows, and the assessed parameters are of limited use as biomarkers for maternal behavior. Copyright © 2014 Elsevier Inc. All rights reserved.
Leiva, T; Cooke, R F; Brandão, A P; Schubach, K M; Batista, L F D; Miranda, M F; Colombo, E A; Rodrigues, R O; Junior, J R G; Cerri, R L A; Vasconcelos, J L M
This study compared vaginal temperature, physiologic, and productive parameters in lactating dairy cows supplemented or not with Omnigen-AF (Phibro Animal Health, Teaneck, NJ) during the summer months in a tropical environment. Thirty-two lactating, primiparous (n = 16) and multiparous (n = 16) pregnant Holstein × Gir cows were ranked by parity, days in milk, body weight, and body condition score (BCS), and assigned to receive (SUPP; n = 16) or not (CON; n = 16) Omnigen-AF (Phibro Animal Health, Teaneck, NJ) at 56 g/cow daily (as-fed basis). During the experimental period (d -6 to 56), cows were maintained in a single drylot pen with ad libitum access to water and a total mixed ration, and milked twice daily. Cows received Omnigen-AF mixed with 200 g of corn (as-fed basis) after the daily morning milking through self-locking head gates, whereas CON cows concurrently received 56 g of kaolin mixed with 200 g of corn. For feed intake evaluation, cows from both treatments were randomly divided in 4 groups of 8 cows each, and allocated to 8 individual feeding stations for 3 d. Intake was evaluated 4 times per group from d 1 to 56. From d -6 to 0, d 15 to 28, and d 43 to 56, cow vaginal temperature was recorded hourly. Environmental temperature-humidity index (THI) was also recorded hourly from d 15 to 28 and d 43 to 56. Cows were evaluated for body weight and BCS on d -6 and 56, individual milk production was recorded daily from d -6 to 56, and milk samples were collected on d -6, 0, 7, 14, 21, 28, 35, 42, 49, and 56 for analyses of somatic cell count and milk components. Blood samples were collected on d -6, -3, 0, 9, 15, 18, 21, 24, 27, 36, 45, 48, 51, 54, and 56. Results from samples or observations collected from d -6 to 0 were included as an independent covariate in each respective analysis. Environmental THI was 74.2 ± 0.5 and cows were exposed to THI >68 for 633 h within a total of 672 h of evaluation. Cows assigned to CON had greater vaginal temperature on d
Maha M. Hady
Full Text Available Aim: The current study aimed to compare the impact of heat stress (HS on some physiological functions and blood oxidative stress biomarkers between dry dairy crossbred (Balady X Friesian cows and buffaloes during Egyptian summer season (July-September. Materials and Methods: A total of 26 healthy animals were equally used in the in the current study. The criterion for cows and buffaloes selection and the management conditions were similar. A total mixed ration to meet the animal's requirements was used, and dry matter intake (DMI was calculated. Ambient temperature, relative humidity, temperature humidity index (THI, respiratory rate, and rectal temperature (RT were daily recorded. Meanwhile, live body weight and body condition score were weekly recorded. Blood samples were collected bi-weekly, and plasma samples were harvested for malondialdehyde (MDA content and enzymatic antioxidants such as glutathione peroxidase, superoxide dismutase, and catalase activities determinations throughout the experimental period (8 weeks - prepartum. Results: The results confirmed, the HS condition, as the THI values ranged from 79.74 to 90.4 throughout the experimental period. In both species, HS increased RT and decreased DMI (<10.5 kg/day and 9.5 kg/day in cows and buffaloes, respectively. Buffaloes seemed to be more affected by the hostile environmental condition of this study compared with their respective cows. Buffaloes had recorded up to 1 °C increase in their RTs in most of the point's period compared to cows. There was a continuous increase in MDA values (194.7 and 208.4 nmol/gHb in buffaloes and cows, respectively, 2 weeks prepartum as the animals come close to parturition with moderate decrements for the enzymatic antioxidant activities in both cows and buffaloes. Conclusion: It can be concluded that during Egyptian's summer season, HS had adversely affected feed intake and consequently animal's production performances.
Rafael Herrera Alvarez
Full Text Available The aim of this study was to compare ovarian response and embryo production of superovulated Bos indicus and Bos taurus cows adapted to the environmental conditions from São Paulo State, Brazil. Ninety non-lactating cows from Caracu ( Bos taurus, n=40 and Nelore (Bos indicus, n=50 were treated with an intravaginal device containing progesterone (1.38 mg; CIDRB ®, Pfizer Animal Health, Montreal, Québec, Canada and 2.5 mg, intramuscularly (IM, of estradiol benzoate (Estrogin®, Farmavet, São Paulo, Brazil. Four days later, all animals were treated with multiple IM injections of 400 IU of FSH (Pluset®, Calier, Spain in decreasing doses (75–75; 75–50; 50–25, and 25–25 IU at 12-h intervals over 4 days. On the seventh day, CIDR-B device was removed and cows received, IM, 150 ìg of cloprostenol (Veteglan®, Calier, Spain. Cows were then inseminated 48 and 62 h after cloprostenol treatment and embryos were recovered non-surgically seven days after first insemination. Differences in the number of corpora lutea (CL number, total number of structures (ova/embryos, and number of transferable embryos were analyzed by Student t test. There was no difference (P > 0.05 in the average number of CL, total ova/embryos and transferable embryos of Caracu (11.4 ± 3.3; 8.6 ± 2.6 e 6.0 ± 2.4 and Nelore (12.0 ± 4.1; 9.0 ± 4.3 e 5.1 ± 2.9 cows, respectively. These results suggest that Caracu and Nelore cows superovulated in tropical climate had similar ovarian responses and embryo production.
Trifković, Julijana; Jovanović, Ljubomir; Đurić, Miloje; Stevanović-Đorđević, Snežana; Milanović, Svetlana; Lazarević, Miodrag; Sladojević, Željko; Kirovski, Danijela
Season may affect calves' thermal comfort and behavior, but the data related to the overall influence of seasonal variations on dams' colostrum and postnatal adaptive capability of calves are limited. The aim of this study was to measure the effects of a 49-day-long low air temperature (LAT) season (5.20 ± 0.46 °C mean air temperature) and a 53-day-long high air temperature (HAT) season (27.40 ± 0.39 °C mean air temperature) on dams' colostrum quality and physiological, biochemical, hormonal, and oxidative stress parameters of their calves during the first 7 days of life. The dams' colostrum was sampled at 2, 14, and 26 h after calving, before feeding of their calves. Calves' blood samples were taken before the first colostrum intake and on days 1, 2, 3, and 7 of life. Calves' physiological parameters were measured on days 0 and 7. HAT season significantly reduced the quality of dams' colostrum. The ingestion of the low-quality colostrum, combined with the thermal discomfort during HAT season, probably provoked impaired physiological, biochemical, hormonal, and oxidative stress parameters in samples taken from the post-colostral calves. Additionally, intravenous glucose tolerance test was performed on day 7, which suggested an enhanced insulin response in HAT season calves. This study highlights the importance of adequate supporting strategies for the care of the late gestation cows and postnatal calves during the HAT season.
Lu, Wei; Wang, Zhenhong
The present studies examined the influence of extraversion on physiological reactivity, recovery, and physiological habituation-sensitization to repeated social stressors. In Study 1, subjective and physiological data were collected from 97 college students who were categorized as high (n = 51) and low (n = 46) on extraversion (NEO-FFI) across five laboratory stages: baseline, stress 1, poststress 1, stress 2, and poststress 2. Results indicated high extraversion (HE) participants exhibited relative lesser heart rate (HR) reactivity and respiratory sinus arrhythmia (RSA) withdrawals to, and more complete HR and RSA recovery after the first social stress, and also exhibited relative lesser HR reactivity to the second social stress. When repeatedly exposed to a social stressor, HE participants showed pronounced systolic blood pressure (SBP) adaptation, low extraversion (LE) participants displayed diastolic blood pressure (DBP) sensitization. In Study 2, data were collected from another 78 participants (HE: n = 40, LE: n = 38) across the same laboratory stages with speech performance videotaped. After controlling for the speech styles, Study 2 found the same HR response and SBP/DBP adaptation pattern across extraversion groups to social stress as Study 1 but not RSA reactivity. These findings suggest extraverts exhibit more adaptive physiological reactivity to recurrent social stressors, which thus might benefit their health. © 2016 Society for Psychophysiological Research.
Whitehead, Andrew; Galvez, Fernando; Zhang, Shujun; Williams, Larissa M; Oleksiak, Marjorie F
Evolutionary solutions to the physiological challenges of life in highly variable habitats can span the continuum from evolution of a cosmopolitan plastic phenotype to the evolution of locally adapted phenotypes. Killifish (Fundulus sp.) have evolved both highly plastic and locally adapted phenotypes within different selective contexts, providing a comparative system in which to explore the genomic underpinnings of physiological plasticity and adaptive variation. Importantly, extensive variation exists among populations and species for tolerance to a variety of stressors, and we exploit this variation in comparative studies to yield insights into the genomic basis of evolved phenotypic variation. Notably, species of Fundulus occupy the continuum of osmotic habitats from freshwater to marine and populations within Fundulus heteroclitus span far greater variation in pollution tolerance than across all species of fish. Here, we explore how transcriptome regulation underpins extreme physiological plasticity on osmotic shock and how genomic and transcriptomic variation is associated with locally evolved pollution tolerance. We show that F. heteroclitus quickly acclimate to extreme osmotic shock by mounting a dramatic rapid transcriptomic response including an early crisis control phase followed by a tissue remodeling phase involving many regulatory pathways. We also show that convergent evolution of locally adapted pollution tolerance involves complex patterns of gene expression and genome sequence variation, which is confounded with body-weight dependence for some genes. Similarly, exploiting the natural phenotypic variation associated with other established and emerging model organisms is likely to greatly accelerate the pace of discovery of the genomic basis of phenotypic variation.
Avendaño-Reyes, L.; Hernández-Rivera, J. A.; Álvarez-Valenzuela, F. D.; Macías-Cruz, U.; Díaz-Molina, R.; Correa-Calderón, A.; Robinson, P. H.; Fadel, J. G.
Heat stress generates a significant economic impact for the dairy industry in arid and semi-arid regions of the world, so that heat abatement is an important issue for dairy producers. The objective of this study was to evaluate effects of two short-term cooling periods on physiological and productive status of lactating Holstein cows during hot ambient temperatures. Thirty-nine multiparous cows were blocked by milk yield and assigned to one of three treatments including: control group (C), cows cooled before milking time (0500 and 1700 h daily, 1 h cooling); AM group, cows cooled at 1000 h and before milking (2 h cooling); and AM + PM group, cows cooled at 1100, 1500 and 2200 h, as well as before milking (4 h cooling). The cooling system was placed in the holding pen which the cows were moved through for cooling. Respiratory rate, and temperatures of thurl and right flank, were lower ( P cows from the AM + PM group than AM and C cows during the morning and afternoon. However, udder temperature was higher in the AM + PM group compared to AM and C groups during the afternoon, although lower than the AM group during the morning. Rectal temperature was similar in all groups. Thyroxin concentrations tended ( P cows had a trend ( P cows.
Kirkeby, Carsten Thure; Græsbøll, Kaare; Nielsen, Søren Saxmose
consequences of continuously adapting the sampling interval in response to the estimated true prevalence in the herd. The key results were that the true prevalence was greatly affected by the hygiene level and to some extent by the test-frequency. Furthermore, the choice of prevalence that will be tolerated...... through a variety of test-strategies, but are challenged by the lack of perfect tests. Frequent testing increases the sensitivity but the costs of testing are a cause of concern for farmers. Here, we used a herd simulation model using milk ELISA tests to evaluate the epidemiological and economic...... in a control scenario had a major impact on the true prevalence in the normal hygiene setting, but less so when the hygiene was poor. The net revenue is not greatly affected by the test-strategy, because of the general variation in net revenues between farms. An exception to this is the low hygiene herd, where...
Chen, Jennifer M; Schütz, Karin E; Tucker, Cassandra B
Dairies commonly mount nozzles above the feed bunk that intermittently spray cows to dissipate heat. These sprinklers use potable water-an increasingly scarce resource-but there is little experimental evidence for how much is needed to cool cows in loose housing. Sprinkler flow rate may affect the efficacy of heat abatement, cattle avoidance of spray (particularly on the head), and water waste. Our objectives were to determine how sprinkler flow rate affects cattle behavioral, physiological, and production responses when cows are given 24-h access to spray in freestall housing, and to evaluate heat abatement in relation to water use. We compared 3 treatments: sprinklers that delivered 1.3 or 4.9L/min (both 3min on and 9min off, 24h/d) and an unsprayed control. Nine pairs of high-producing lactating Holstein cows received each treatment at a shaded feed bunk for 2d in a replicated 3×3 Latin square design [air temperature (T): 24-h maximum=33±3°C, mean ± SD]. Cows spent 5.8±0.9h/24h (mean ± SD) at the feed bunk overall, regardless of treatment. With few exceptions, cows responded similarly to the 1.3 and 4.9L/min flow rates. Sprinklers resulted in visits to the feed bunk that were on average 23 to 27% longer and 13 to 16% less frequent compared with the control, perhaps because cows avoided walking through spray. Indeed, when the sprinklers were on, cows left the feed bunk half as often as expected by chance, and when cows chose to walk through spray, they lowered their heads on average 1.7- to 3-fold more often than in the control. Despite possible reluctance to expose their heads to spray, cows did not avoid sprinklers overall. In warmer weather, cows spent more time at the feed bunk when it had sprinklers (on average 19 to 21min/24h for each 1°C increase in T), likely for heat abatement benefits. Compared with the control, sprinklers resulted in 0.3 to 0.7°C lower body temperature from 1300 to 1500h and 1700 to 2000h overall and attenuated the rise in this
Full Text Available Paratuberculosis is a chronic infection that in dairy cattle causes reduced milk yield, weight loss, and ultimately fatal diarrhea. Subclinical animals can excrete bacteria (Mycobacterium avium ssp. paratuberculosis, MAP in feces and infect other animals. Farmers identify the infectious animals through a variety of test-strategies, but are challenged by the lack of perfect tests. Frequent testing increases the sensitivity but the costs of testing are a cause of concern for farmers. Here, we used a herd simulation model using milk ELISA tests to evaluate the epidemiological and economic consequences of continuously adapting the sampling interval in response to the estimated true prevalence in the herd. The key results were that the true prevalence was greatly affected by the hygiene level and to some extent by the test-frequency. Furthermore, the choice of prevalence that will be tolerated in a control scenario had a major impact on the true prevalence in the normal hygiene setting, but less so when the hygiene was poor. The net revenue is not greatly affected by the test-strategy, because of the general variation in net revenues between farms. An exception to this is the low hygiene herd, where frequent testing results in lower revenue. When we look at the probability of eradication, then it is correlated with the testing frequency and the target prevalence during the control phase. The probability of eradication is low in the low hygiene herd, and a test-and-cull strategy should probably not be the primary strategy in this herd. Based on this study we suggest that, in order to control MAP, the standard Danish dairy farm should use an adaptive strategy where a short sampling interval of three months is used when the estimated true prevalence is above 1%, and otherwise use a long sampling interval of one year.
Teama, F.E.I.; Osman, S.F.; El-Tarabany, A.A.
The aim of the present study is to examine the physiological effect of supplemental palm oil fatty acids- Ca salt and buffer on some biochemical and hormonal levels, milk yield and composition for high yielding Holstein Friesian cows during transition period. Thirty six Holstein Friesian cows, aged 53±1.4 months and 739±19.86 kg body weight, were used. The cows were randomly divided into three groups; 12 cows each, control group, the second group received 4% calcium salt palm oil fatty acid (Ca-POFA) and the third group received Ca-POFA + buffer ( 0.5% sodium bicarbonate) for three weeks before and after parturition (transition period). Blood samples were collected at 21, 14, 7 and 2 days before and 2, 7, 14 and 21 days after parturition to assess total protein, albumin, globulin, glucose, cholesterol, creatinine, total lipids and total cholesterol in addition to cortisol and progesterone hormones. Milk yield were recorded for each animal and samples were taken after parturition at different intervals (7, 14 and 21 days) to assess milk composition. Significant decreases in total protein, globulin, total lipids, cortisol and progesterone levels in post-partum cows compared to pre-partum were improved after Ca-POFA + buffer supplementation. On the other hand, increased levels of serum albumin, cholesterol and creatinine were recorded in post-partum period which returned to the normal level as shown in albumin only after treatment with Ca-POFA + buffer. A continuous increase in cholesterol and creatinine levels was observed as a result of supplementation of Ca-POFA + buffer (0.5% sodium bicarbonate). Significant improvements in milk yield, 4% FCM and GE were determined after Ca-POFA + buffer supplementation whereas non-significant changes were recorded in total solids, fat, protein and ash content of milk. In the present study, supplementation of cows in transition period with Ca-POFA + buffer (0.5% sodium bicarbonate) can improve the levels of some blood components
Moyes, Kasey; Larsen, Torben; Ingvartsen, Klaus Lønne
Physiological imbalance (PI) is a situation in which physiological parameters deviate from the normal and cows consequently have an increased risk of developing production diseases and reduced production or reproduction. The objectives of this work were (1) to generate an index for PI based...
The central aim of this thesis is to investigate whether individual dairy cows display different and coherent patterns of physiological and behavioural stress responses. Such responses enable them to successful adapt in a changing environment.
In Chapter 1, current
Calfapietra, Carlo; Peñuelas, Josep; Niinemets, Ülo
Urban environments that are stressful for plant function and growth will become increasingly widespread in future. In this opinion article, we define the concept of 'urban plant physiology', which focuses on plant responses and long term adaptations to urban conditions and on the capacity of urban vegetation to mitigate environmental hazards in urbanized settings such as air and soil pollution. Use of appropriate control treatments would allow for studies in urban environments to be comparable to expensive manipulative experiments. In this opinion article, we propose to couple two approaches, based either on environmental gradients or manipulated gradients, to develop the concept of urban plant physiology for assessing how single or multiple environmental factors affect the key environmental services provided by urban forests. Copyright © 2014 Elsevier Ltd. All rights reserved.
Metabolic disorders are a key problem in the transition period of dairy cows and often appear before the onset of further health problems. They mainly derive from difficulties the animals have in adapting to changes and disturbances occurring both outside and inside the organisms and due to varying gaps between nutrient supply and demand. Adaptation is a functional and target-oriented process involving the whole organism and thus cannot be narrowed down to single factors. Most problems which challenge the organisms can be solved in a number of different ways. To understand the mechanisms of adaptation, the interconnectedness of variables and the nutrient flow within a metabolic network need to be considered. Metabolic disorders indicate an overstressed ability to balance input, partitioning and output variables. Dairy cows will more easily succeed in adapting and in avoiding dysfunctional processes in the transition period when the gap between nutrient and energy demands and their supply is restricted. Dairy farms vary widely in relation to the living conditions of the animals. The complexity of nutritional and metabolic processes Animals 2015, 5 979 and their large variations on various scales contradict any attempts to predict the outcome of animals' adaptation in a farm specific situation. Any attempts to reduce the prevalence of metabolic disorders and associated production diseases should rely on continuous and comprehensive monitoring with appropriate indicators on the farm level. Furthermore, low levels of disorders and diseases should be seen as a further significant goal which carries weight in addition to productivity goals. In the long run, low disease levels can only be expected when farmers realize that they can gain a competitive advantage over competitors with higher levels of disease.
Lane, H. W.; LeBlanc, A. D.; Putcha, L.; Whitson, P. A.
Space flight provides a model for the study of healthy individuals undergoing unique stresses. This review focuses on how physiological adaptations to weightlessness may affect nutrient and food requirements in space. These adaptations include reductions in body water and plasma volume, which affect the renal and cardiovascular systems and thereby fluid and electrolyte requirements. Changes in muscle mass and function may affect requirements for energy, protein and amino acids. Changes in bone mass lead to increased urinary calcium concentrations, which may increase the risk of forming renal stones. Space motion sickness may influence putative changes in gastro-intestinal-hepatic function; neurosensory alterations may affect smell and taste. Some or all of these effects may be ameliorated through the use of specially designed dietary countermeasures.
da Costa, Antônio Nélson Lima; Feitosa, José Valmir; Júnior, Péricles Afonso Montezuma; de Souza, Priscila Teixeira; de Araújo, Airton Alencar
This study compared two breed groups of Girolando (½ Holstein ½ Gyr vs. ¾ Holstein ¼ Gyr) through analysis of physiological, productive, and reproductive parameters to determine the group best suited to rearing in a semiarid tropical climate. The experiment was conducted at the Companhia de Alimentos do Nordeste (CIALNE) farm, in the municipality of Umirim, State of Ceará, Brazil. Eighty cows were used in a 2 × 2 factorial study; 40 of each breed group were kept under an extensive system during the wet season and an intensive system during the dry season. The collection of physiological data and blood samples were obtained in the afternoon after milking. Rectal temperature (RT), surface temperature (ST), and respiratory rate (RR) were obtained for each cow after milking. Blood samples were obtained by tail vein puncture and were determined triiodothyronine (T3) and thyroxine (T4) and cortisol. The environmental parameters obtained were relative humidity (RH) and air temperature (AT), and from these, a temperature and humidity index (THI) was calculated. Pregnancy diagnosis (PD) was determined by ultrasonography 30 days after artificial insemination (AI). The milk production of each cow was recorded with automated milkings in the farm. The variables were expressed as mean and standard error, evaluated by ANOVA at 5 % probability using the Proc GLM of SAS. Chi-square test at 5 % probability was applied to data of pregnancy rate (PR) and the number of AI's to obtain pregnancy. It can be concluded that the breed group ½ Holstein ½ Gyr is most suited for farming under conditions of thermal stress.
Signore, Anthony V.; Fago, Angela; Weber, Roy E.
sensitivity that can maintain adequate O2 delivery at low temperatures. This phenotype has primarily been attributed to the binding of additional allosteric effectors to the Hb moiety relative to those of non-cold adapted species. However, we find that Hb from the extinct Steller’s sea cow binds fewer...... paenungulate Hbs (woolly mammoth, Asian elephant, Steller’s sea cow, dugong, ancestral dugongid, and Florida manatee) in the presence of serially increasing Cl- and 2,3-diphosphoglycerate (DPG) concentrations at both 25 and 37C. Quantitation of effector binding revealed that the number of oxygenation...
Martinez, N; Sinedino, L D P; Bisinotto, R S; Ribeiro, E S; Gomes, G C; Lima, F S; Greco, L F; Risco, C A; Galvão, K N; Taylor-Rodriguez, D; Driver, J P; Thatcher, W W; Santos, J E P
The objectives were to study the effects of induced subclinical hypocalcemia [SCH, blood ionized Ca (iCa(2+)) dairy cows. Ten nonpregnant, nonlactating Holstein cows were blocked by lactation and assigned randomly to a normocalcemic (NC; intravenous infusion of 0.9% NaCl i.v. plus 43 g of oral Ca, as Ca sulfate and Ca chloride, at -1 and 11h) or an induced SCH [SCHI, 5% ethylene glycol tetraacetic acid (EGTA), a selective iCa(2+) chelator, intravenous infusion] treatment for 24h, using a crossover design. The sequence of treatments was either NC-SCHI or SCHI-NC, with a 6-d washout period. Ionized Ca was evaluated before, hourly during the infusion period, and at 48 and 72 h, to monitor concentrations and adjust the rate of infusion, maintaining blood iCa(2+) insulin in plasma, and urinary excretion of Ca. Total and differential leukocyte count in blood was also performed. The concentration of cytosolic iCa(2+) in neutrophils and lymphocytes was quantified and neutrophil function was assayed in vitro. Infusion of a 5% EGTA solution successfully induced SCH in all SCHI cows, resulting in decreased blood iCa(2+) concentrations throughout the 24-h treatment period (0.77 ± 0.01 vs. 1.26 ± 0.01 mM iCa(2+)). Induction of SCH reduced dry matter intake on the day of infusion (5.3 ± 0.8 vs. 9.1 ± 0.8 kg/d) and rumen contractions (1.9 ± 0.2 vs. 2.7 ± 0.2 contractions/2 min) for the last 12h of infusion. Cows in SCHI had decreased plasma insulin concentration (1.44 ± 0.23 vs. 2.32 ± 0.23 ng/mL) evident between 6 and 18 h after the beginning of the infusion, accompanied by increased concentrations of glucose (4.40 ± 0.04 vs. 4.17 ± 0.04 mM). Plasma nonesterified fatty acids concentration was greater for SCHI than NC cows (0.110 ± 0.019 vs. 0.061 ± 0.014 mM). Neutrophils of cows in SCHI had a faster decrease in cytosolic iCa(2+) after stimulation with ionomycin (9.9 ± 1.0 vs. 13.6 ± 1.4 Fluo-4:Fura Red post-end ratio) in vitro. Furthermore, induction of SCH reduced
Mankowski, Robert T.; Anton, Stephen D.; Buford, Thomas W.; Leeuwenburgh, Christiaan
Adaptive responses to exercise training (ET) are crucial in maintaining physiological homeostasis and health span. Exercise-induced aerobic bioenergetic reactions in mitochondria and cytosol increase production of reactive oxygen species (ROSs), where excess of ROS can be scavenged by enzymatic as well as non-enzymatic antioxidants to protect against deleterious oxidative stress. Free radicals, however, have recently been recognized as crucial signaling agents that promote adaptive mechanisms to ET, such as mitochondrial biogenesis, antioxidant (AO) enzyme activity defense system upregulation, insulin sensitivity, and glucose uptake in skeletal muscle. Commonly used non-enzymatic AO supplements, such as vitamins C and E, a-lipoic acid, and polyphenols, in combination with ET, have been proposed as ways to prevent exercise-induced oxidative stress and hence improve adaptation responses to endurance training. Preclinical and clinical studies to date have shown inconsistent results indicating either positive or negative effects of endurance training combined with different blends of AO supplements (mostly vitamins C and E and a-lipoic acid) on redox status, mitochondrial biogenesis pathways, and insulin sensitivity. Preclinical reports on ET combined with resveratrol, however, have shown consistent positive effects on exercise performance, mitochondrial biogenesis, and insulin sensitivity, with clinical trials reporting mixed effects. Relevant clinical studies have been few and have used inconsistent results and methodology (types of compounds, combinations, and supplementation time). The future studies would investigate the effects of specific antioxidants and other popular supplements, such as a-lipoic acid and resveratrol, on training effects in humans. Of particular importance are older adults who may be at higher risk of age-related increased oxidative stress, an impaired AO enzyme defense system, and comorbidities such as hypertension, insulin resistance, and
Bjerre-Harpøth, V; Friggens, N C; Thorup, V M; Larsen, T; Damgaard, B M; Ingvartsen, K L; Moyes, K M
Physiological imbalance (PI) is a situation in which physiological parameters deviate from the normal, and cows consequently have an increased risk of developing production diseases and reduced production or reproduction. Our objectives were to (1) determine the effect of stage of lactation and milk yield on metabolic and production responses of cows during a nutrient restriction period to experimentally increase PI; (2) identify major metabolites that relate to degree of PI; and (3) identify potential biomarkers in milk for on-farm detection of PI throughout lactation. Forty-seven Holstein cows in early [n=14; 49±22 d in milk (DIM); parity=1.6±0.5], mid (n=15; 159±39 DIM; parity=1.5±0.5), and late (n=18; 273±3 DIM; parity=1.3±0.5) lactation were used. Prior to restriction, all cows were fed the same total mixed ration ad libitum. All cows were then nutrient restricted for 4 d by supplementing the ration with 60% wheat straw to induce PI. After restriction, cows returned to full feed. Daily milk yield was recorded and composite milk samples were analyzed for fat, protein, lactose, citrate, somatic cells, uric acid, alkaline phosphatase, β-hydroxybutyrate (BHBA), and milk urea nitrogen. Blood was collected daily and analyzed for metabolites: nonesterified fatty acids (NEFA), BHBA, glucose, plasma urea nitrogen, and insulin. The revised quantitative insulin sensitivity check index (RQUICKI) was calculated for each cow. Liver biopsies collected before and during restriction were analyzed for triglycerides, glycogen, phospholipids, glucose, and total lipid content. A generalized linear mixed model was used to determine the effect of stage of lactation on responses during restriction. Regression analyses were used to examine the effect of pre-restriction levels on changes during restriction. Similar decreases in milk yield among groups indicate that the capacity of individual responses is dependent on milk yield but the coping strategies used are dependent on
Wilson Andrés Berragán-Hernández
Full Text Available The aim of this work was to analyze changes of physiological and metabolic parameters as indicators of heat stress of cows in pasture systems. The research was carried out from 2011 to 2012 at the Turipaná Agricultural Research Center of Corpoica located in the Caribbean region in Cereté–Colombia. Environmental temperature (T and relative humidity (H were determined, as well as and rectal temperature (RT, skin temperature (ST, respiratory frequency (RF and the acid-basic status of animals. The variables were measured in the morning (6:00 h and in the afternoon (13:00 h. Significant Statistical differences were observed (p<0.05 in environmental temperature treatments (T with 7% and 6% less temperature in p-Arbus-Arbor y p-Arbor, respectively, compared with grass treatment. There was a significant hour effect on T and H (p<0.05 and a significant treatment-hour interaction on T (p<0.05. TP and FR showed a significant treatment-hour interaction per hour (6:00/13:00 h. The results show a positive effect of shadow from trees on the physiological variables. The negative effects observed on the physiological variables of unshaded treatments impacted in a minimal way the metabolic variables suggesting homeostatic responses in the animals under the evaluated stressful environmental conditions.
Alimohammadi, Nasrollah; Maleki, Bibi; Shahriari, Mohsen; Chitsaz, Ahmad
Stroke is a stressful event with several functional, physical, psychological, social, and economic problems that affect individuals' different living balances. With coping strategies, patients try to control these problems and return to their natural life. The aim of this study is to investigate the effect of a care plan based on Roy adaptation model biological dimension on stroke patients' physiologic adaptation level. This study is a clinical trial in which 50 patients, affected by brain stroke and being admitted in the neurology ward of Kashani and Alzahra hospitals, were randomly assigned to control and study groups in Isfahan in 2013. Roy adaptation model care plan was administered in biological dimension in the form of four sessions and phone call follow-ups for 1 month. The forms related to Roy adaptation model were completed before and after intervention in the two groups. Chi-square test and t-test were used to analyze the data through SPSS 18. There was a significant difference in mean score of adaptation in physiological dimension in the study group after intervention (P adaptation in the patients affected by brain stroke in the study and control groups showed a significant increase in physiological dimension in the study group by 47.30 after intervention (P adaptation model biological dimension care plan can result in an increase in adaptation in patients with stroke in physiological dimension. Nurses can use this model for increasing patients' adaptation.
Moyes, Kasey; Bendixen, Emøke; Codrea, Marius Cosmin
the ration with 60% wheat straw. Liver biopsies were collected −1 and 3 d relative to restriction. Before restriction, an index for PI was calculated based on plasma nonesterified fatty acids, β-hydroxybutyrate, and glucose concentrations. Within E and M cows, a subsets of 6 cow was classified as having...... as potential hepatic biomarkers for PI for cows during early lactation and alcohol dehydrogenase-4 and methylmalonate-semialdehyde dehydrogenase for cows in mid lactation. This preliminary study identified new biomarkers in liver for PI and provided a better understanding of the differences in coping...
Hammer, Graeme L; van Oosterom, Erik; McLean, Greg; Chapman, Scott C; Broad, Ian; Harland, Peter; Muchow, Russell C
Progress in molecular plant breeding is limited by the ability to predict plant phenotype based on its genotype, especially for complex adaptive traits. Suitably constructed crop growth and development models have the potential to bridge this predictability gap. A generic cereal crop growth and development model is outlined here. It is designed to exhibit reliable predictive skill at the crop level while also introducing sufficient physiological rigour for complex phenotypic responses to become emergent properties of the model dynamics. The approach quantifies capture and use of radiation, water, and nitrogen within a framework that predicts the realized growth of major organs based on their potential and whether the supply of carbohydrate and nitrogen can satisfy that potential. The model builds on existing approaches within the APSIM software platform. Experiments on diverse genotypes of sorghum that underpin the development and testing of the adapted crop model are detailed. Genotypes differing in height were found to differ in biomass partitioning among organs and a tall hybrid had significantly increased radiation use efficiency: a novel finding in sorghum. Introducing these genetic effects associated with plant height into the model generated emergent simulated phenotypic differences in green leaf area retention during grain filling via effects associated with nitrogen dynamics. The relevance to plant breeding of this capability in complex trait dissection and simulation is discussed.
Popović, Sinisa; Horvat, Marko; Kukolja, Davor; Dropuljić, Branimir; Cosić, Kresimir
Significant proportion of psychological problems related to combat stress in recent large peacekeeping operations underscores importance of effective methods for strengthening the stress resistance of military personnel. Adaptive control of virtual reality (VR) stimulation, based on estimation of the subject's emotional state from physiological signals, may enhance existing stress inoculation training (SIT). Physiology-driven adaptive VR stimulation can tailor the progress of stressful stimuli delivery to the physiological characteristics of each individual, which is indicated for improvement in stress resistance. Therefore, following an overview of SIT and its applications in the military setting, generic concept of physiology-driven adaptive VR stimulation is presented in the paper. Toward the end of the paper, closed-loop adaptive control strategy applicable to SIT is outlined.
This viewgraph presentation reviews human physiological responses to spaceflight, and the countermeasures taken to prevent adverse effects of manned space flight. The topics include: 1) Human Spaceflight Experience; 2) Human Response to Spaceflight; 3) ISS Expeditions 1-16; 4) Countermeasure; and 5) Biomedical Data;
Ilardo, Melissa A; Moltke, Ida; Korneliussen, Thorfinn S
Understanding the physiology and genetics of human hypoxia tolerance has important medical implications, but this phenomenon has thus far only been investigated in high-altitude human populations. Another system, yet to be explored, is humans who engage in breath-hold diving. The indigenous Bajau...
Voronina, L.; Morachevskaya, E.
It is known, that brassinosteroids are capable in small quantities (10 - 12-10 - 7M) to optimize physiology-biochemical processes in plants in stressful conditions. the aim of this study was to investigate the role of anti stress and protective properties of phyto hormone 24-epibrassinolide (24-epiBS). in view of its functional features and biological activity. (Author)
Yu, Zhi-jun; Yang, Xiao-long; Chen, Jie; Liu, Jing-ze
The current paper introduces the recent research and development on the cryobiology of ticks, based on their overwinter behavior strategy and biochemical and physiological adaptation mechanisms, and provides detail information on the cold hardiness, biochemical and physiological mechanisms, the relationship between cold hardiness and diapause, which will give theoretical clues for subsequent research on the molecular regulation of cold hardiness of ticks.
Brown Sam P
Full Text Available Abstract Background Confronted with well-defended, novel hosts, should an enemy invest in avoidance of these hosts (behavioral adaptation, neutralization of the defensive innovation (physiological adaptation or both? Although simultaneous investment in both adaptations may first appear to be redundant, several empirical studies have suggested a reinforcement of physiological resistance to host defenses with additional avoidance behaviors. To explain this paradox, we develop a mathematical model describing the joint evolution of behavioral and physiological adaptations on the part of natural enemies to their host defenses. Our specific goals are (i to derive the conditions that may favor the simultaneous investment in avoidance and physiological resistance and (ii to study the factors that govern the relative investment in each adaptation mode. Results Our results show that (i a simultaneous investment may be optimal if the fitness costs of the adaptive traits are accelerating and the probability of encountering defended hosts is low. When (i holds, we find that (ii the more that defended hosts are rare and/or spatially aggregated, the more behavioral adaptation is favored. Conclusion Despite their interference, physiological resistance to host defensive innovations and avoidance of these same defenses are two strategies in which it may be optimal for an enemy to invest in simultaneously. The relative allocation to each strategy greatly depends on host spatial structure. We discuss the implications of our findings for the management of invasive plant species and the management of pest resistance to new crop protectants or varieties.
Thompson-Crispi, K A; Hine, B; Quinton, M; Miglior, F; Mallard, B A
The objective of this study was to use previously calculated estimated breeding values for cell- (CMIR) and antibody-mediated immune responses (AMIR) to determine associations between immune response (IR) and economically important diseases of dairy cattle. In total, 699 Holsteins were classified as high, average, or low for CMIR, AMIR, and overall IR (combined CMIR and AMIR), and associations with mastitis, metritis, ketosis, displaced abomasums, and retained fetal membranes were determined. The incidence of mastitis was higher among average cows as compared with cows classified as high AMIR [odds ratio (OR)=2.5], high CMIR (OR=1.8), or high IR (OR=1.8). Low-CMIR cows had a higher incidence of metritis (OR=11.3) and low-IR cows had a higher incidence of displaced abomasum (OR=4.1) and retained fetal membrane (OR=2.8) than did average responders. Results of this study show that cows classified as high immune responders have lower occurrence of disease, suggesting that breeding cattle for enhanced IR may be a feasible approach to decrease the incidence of infectious and metabolic diseases in the dairy industry. Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Costa Mário J.
Full Text Available The purpose of this systematic review was to summarize longitudinal studies on swimming physiology and get implications for daily practice. A computerized search of databases according to the PRISMA statement was employed. Studies were screened for eligibility on inclusion criteria: (i present two testing points; (ii on swimming physiology; (iii using adult elite swimmers; (iv no case-studies or with small sample sizes. Two independent reviewers used a checklist to assess the methodological quality of the studies. Thirty-four studies selected for analysis were gathered into five main categories: blood composition (n=7, endocrine secretion (n=11, muscle biochemistry (n=7, cardiovascular response (n=8 and the energetic profile (n=14. The mean quality index was 10.58 ± 2.19 points demonstrating an almost perfect agreement between reviewers (K = 0.93. It can be concluded that the mixed findings in the literature are due to the diversity of the experimental designs. Micro variables obtained at the cellular or molecular level are sensitive measures and demonstrate overtraining signs and health symptoms. The improvement of macro variables (i.e. main physiological systems is limited and may depend on the athletes’ training background and experience.
Cosić, Kresimir; Popović, Sinisa; Kukolja, Davor; Horvat, Marko; Dropuljić, Branimir
The significant proportion of severe psychological problems related to intensive stress in recent large peacekeeping operations underscores the importance of effective methods for strengthening the prevention and treatment of stress-related disorders. Adaptive control of virtual reality (VR) stimulation presented in this work, based on estimation of the person's emotional state from physiological signals, may enhance existing stress inoculation training (SIT). Physiology-driven adaptive VR stimulation can tailor the progress of stressful stimuli delivery to the physiological characteristics of each individual, which is indicated for improvement in stress resistance. Following an overview of physiology-driven adaptive VR stimulation, its major functional subsystems are described in more detail. A specific algorithm of stimuli delivery applicable to SIT is outlined.
Celi, Francesco S; Le, Trang N; Ni, Bin
In homoeothermic organisms, the preservation of core temperature represents a primal function, and its costs in terms of energy expenditure can be considerable. In modern humans, the endogenous thermoregulation mechanisms have been replaced by clothing and environmental control, and the maintenance of thermoneutrality has been successfully achieved by manipulation of the micro- and macroenvironment. The rediscovery of the presence and activity of brown adipose tissue in adult humans has renewed the interest on adaptive thermogenesis (AT) as a means to facilitate weight loss and improve carbohydrate metabolism. The aim of this review is to describe the recent advancements in the study of this function, and to assess the potential and limitations of exploiting AT for environmental/behavioral, and pharmacological interventions. Copyright © 2015 Elsevier Ltd. All rights reserved.
Zhang, L; Ying, S J; An, W J; Lian, H; Zhou, G B; Han, Z Y
This study aimed to determine whether feeding betaine to cows elevates their production performance during summer heat stress. Thirty-two lactating Holstein cows were randomly divided into 4 groups: the control group, which received a total mixed ration (TMR), and 3 experimental groups that received TMR blended with 10 g/day (group I), 15 g/day (group II), and 20 g/day (group III) betaine for 8 weeks. Milk and blood were sampled throughout the experimental period. The average maximum and minimum air temperatures were 28.3 and 24.1°C, respectively. The average temperature-humidity index was 78.6 units. The results showed that feeding betaine to cows increased feed intake, milk yield, milk lactose, milk protein, plasma cortisol, glutathione peroxidase, superoxide dismutase, and malondialdehyde levels (Pcows increases their milk performance and improves their antioxidant capacity; these processes help relieve the cow from heat stress. In conclusion, supplementing dairy cows with 15 g/day betaine generated the most positive influence on performance and productivity, and hence caused the greatest reduction in heat stress.
de Paula Xavier de Andrade, Rafael; de Andrade Ferreira, Marcelo; de Azevedo, Marcílio; da Silva, Emmanuelle Cordeiro; Urbano, Stela Antas; da Conceição, Maria Gabriela; de Lima Silva, Janaina
The effects of time of feed delivery (14.00 hours; 14.00 and 20.00 hours; 16.00 and 18.00 hours) on the physiological parameters, ingestive behavior, nutrient intake and production of lactating cows maintained in a semi-arid region during the hot season were evaluated. Regardless of treatment, all animals received the first feeding supply at 06.00 hours. Eight cows with an average body weight of 600 kg, average milk yield of 20 kg/day and 80 days in milk were utilized. The rectal temperature, respiratory rate and sweating rate were not affected (P > 0.05), with average values of 38.5°C, 53.8 movements/min and 104 g/m 2 /h respectively. There was no effect (P > 0.05) on the eating time (314 min/day), ruminating time (564 min/day), drinking time (61 min/day) and idle time (502 min/day). Similarly, the intake of nutrients and performance of lactating cows were not affected (P > 0.05), with average dry matter intake of 19.8 kg/day, 4% fat-corrected milk of 20.6 kg/day and milk fat concentration of 4.03 g/100 g. Since the behavior and performance have not altered, any times of feed delivery evaluated could be used to crossbred Holstein × Zebu cows maintained on a feedlot in semi-arid regions during the hot season. © 2016 Japanese Society of Animal Science.
Ingvartsen, Klaus Lønne; Kuhla, Björn
The high-yielding dairy cow faces major adaptions during the transition period from late pregnancy to early lactation where physiological changes occur in support of the dramatically increase in milk yield. The coordinated physiological changes secure mobilization of nutrients and energy from the...
During the transition period, dairy cows experience a negative energy balance (NEB) caused by the high energy requirement for milk yield, while feed intake is limited. Severity of the NEB has been associated with an increased incidence of metabolic disorders and infectious
Zupanska, Agata K.; Schultz, Eric R.; Yao, JiQiang; Sng, Natasha J.; Zhou, Mingqi; Callaham, Jordan B.; Ferl, Robert J.; Paul, Anna-Lisa
Scientific access to spaceflight and especially the International Space Station has revealed that physiological adaptation to spaceflight is accompanied or enabled by changes in gene expression that significantly alter the transcriptome of cells in spaceflight. A wide range of experiments have shown that plant physiological adaptation to spaceflight involves gene expression changes that alter cell wall and other metabolisms. However, while transcriptome profiling aptly illuminates changes in gene expression that accompany spaceflight adaptation, mutation analysis is required to illuminate key elements required for that adaptation. Here we report how transcriptome profiling was used to gain insight into the spaceflight adaptation role of Altered response to gravity 1 (Arg1), a gene known to affect gravity responses in plants on Earth. The study compared expression profiles of cultured lines of Arabidopsis thaliana derived from wild-type (WT) cultivar Col-0 to profiles from a knock-out line deficient in the gene encoding ARG1 (ARG1 KO), both on the ground and in space. The cell lines were launched on SpaceX CRS-2 as part of the Cellular Expression Logic (CEL) experiment of the BRIC-17 spaceflight mission. The cultured cell lines were grown within 60 mm Petri plates in Petri Dish Fixation Units (PDFUs) that were housed within the Biological Research In Canisters (BRIC) hardware. Spaceflight samples were fixed on orbit. Differentially expressed genes were identified between the two environments (spaceflight and comparable ground controls) and the two genotypes (WT and ARG1 KO). Each genotype engaged unique genes during physiological adaptation to the spaceflight environment, with little overlap. Most of the genes altered in expression in spaceflight in WT cells were found to be Arg1-dependent, suggesting a major role for that gene in the physiological adaptation of undifferentiated cells to spaceflight.
Balen, V.A.L. van; Gansewinkel, T.A.G. van; Haas, S.; Kuijk, S.M.J. van; Drongelen, J. van; Ghossein-Doha, C.; Spaanderman, M.E.A.
OBJECTIVES: To establish reference values for flow-mediated dilatation (FMD) and brachial artery diameter (BAD) in pregnancy and to provide insight into the physiological and pathological course of endothelial adaptation throughout human singleton pregnancy. METHODS: A meta-analysis was performed
Weigle, David S.; Buben, Amelia; Burke, Caitlin C.; Carroll, Nels D.; Cook, Brett M.; Davis, Benjamin S.; Dubowitz, Gerald; Fisher, Rian E.; Freeman, Timothy C.; Gibbons, Stephen M.; Hansen, Hale A.; Heys, Kimberly A.; Hopkins, Brittany; Jordan, Brittany L.; McElwain, Katherine L.; Powell, Frank L.; Reinhart, Katherine E.; Robbins, Charles D.; Summers, Cameron C.; Walker, Jennifer D.; Weber, Steven S.; Weinheimer, Caroline J.
In this article, an experiential learning activity is described in which 19 university undergraduates made experimental observations on each other to explore physiological adaptations to high altitude. Following 2 wk of didactic sessions and baseline data collection at sea level, the group ascended to a research station at 12,500-ft elevation.…
Full Text Available Eccentric exercise is characterized by initial unfavorable effects such as subcellular muscle damage, pain, reduced fiber excitability, and initial muscle weakness. However, stretch combined with overload, as in eccentric contractions, is an effective stimulus for inducing physiological and neural adaptations to training. Eccentric exercise-induced adaptations include muscle hypertrophy, increased cortical activity, and changes in motor unit behavior, all of which contribute to improved muscle function. In this brief review, neuromuscular adaptations to different forms of exercise are reviewed, the positive training effects of eccentric exercise are presented, and the implications for training are considered.
Ceciliani, Fabrizio; Lecchi, Cristina; Urh, Christiane; Sauerwein, Helga
The transition from late pregnancy to early lactation is a critical period in a dairy cow's life due to the rapidly increasing drain of nutrients from the maternal organism towards the foetus and into colostrum and milk. In order to cope with the challenges of parturition and lactation, comprehensive adaptive reactions comprising the endocrine and the immune system need to be accomplished. There is high variation in this coping ability and both metabolic and infectious diseases, summarized as "production diseases", such as hypocalcaemia (milk fever), fatty liver syndrome, laminitis and ketosis, may occur and impact welfare, productive lifespan and economic outcomes. Proteomics and metabolomics have emerged as valuable techniques to characterize proteins and metabolite assets from tissue and biological fluids, such as milk, blood and urine. In this review we provide an overview on metabolic status and physiological changes during the transition period and the related production diseases in dairy cows, and summarize the state of art on proteomics and metabolomics of biological fluids and tissues involved in metabolic stress during the peripartum period. We also provide a current and prospective view of the application of the recent achievements generated by omics for biomarker discovery and their potential in diagnosis. For high-yielding dairy cows there are several "occupational diseases" that occur mainly during the metabolic challenges related to the transition from pregnancy to lactation. Such diseases and their sequelae form a major concern for dairy production, and often lead to early culling of animals. Beside the economical perspective, metabolic stress may severely influence animal welfare. There is a multitude of studies about the metabolic backgrounds of such so called production diseases like ketosis, fatty liver, or hypocalcaemia, although the investigations aiming to assess the complexity of the pathophysiological reactions are largely focused on gene
Rivera-Brown, Anita M; Frontera, Walter R
Physical activity and fitness are associated with a lower prevalence of chronic diseases, such as heart disease, cancer, high blood pressure, and diabetes. This review discusses the body's response to an acute bout of exercise and long-term physiological adaptations to exercise training with an emphasis on endurance exercise. An overview is provided of skeletal muscle actions, muscle fiber types, and the major metabolic pathways involved in energy production. The importance of adequate fluid intake during exercise sessions to prevent impairments induced by dehydration on endurance exercise, muscular power, and strength is discussed. Physiological adaptations that result from regular exercise training such as increases in cardiorespiratory capacity and strength are mentioned. The review emphasizes the cardiovascular and metabolic adaptations that lead to improvements in maximal oxygen capacity. Copyright © 2012 American Academy of Physical Medicine and Rehabilitation. Published by Elsevier Inc. All rights reserved.
Beef cow efficiency, a century’s old debate, on what the criteria, certain phenotypic traits, and definition of an “efficient” cow really should be. However, we do know that energy utilization by the cow herd is proportionally large compared to the rest of the sector. This requirement accounts up to...
MacInnis, Martin J; Gibala, Martin J
Interval exercise typically involves repeated bouts of relatively intense exercise interspersed by short periods of recovery. A common classification scheme subdivides this method into high-intensity interval training (HIIT; 'near maximal' efforts) and sprint interval training (SIT; 'supramaximal' efforts). Both forms of interval training induce the classic physiological adaptations characteristic of moderate-intensity continuous training (MICT) such as increased aerobic capacity (V̇O2 max ) and mitochondrial content. This brief review considers the role of exercise intensity in mediating physiological adaptations to training, with a focus on the capacity for aerobic energy metabolism. With respect to skeletal muscle adaptations, cellular stress and the resultant metabolic signals for mitochondrial biogenesis depend largely on exercise intensity, with limited work suggesting that increases in mitochondrial content are superior after HIIT compared to MICT, at least when matched-work comparisons are made within the same individual. It is well established that SIT increases mitochondrial content to a similar extent to MICT despite a reduced exercise volume. At the whole-body level, V̇O2 max is generally increased more by HIIT than MICT for a given training volume, whereas SIT and MICT similarly improve V̇O2 max despite differences in training volume. There is less evidence available regarding the role of exercise intensity in mediating changes in skeletal muscle capillary density, maximum stroke volume and cardiac output, and blood volume. Furthermore, the interactions between intensity and duration and frequency have not been thoroughly explored. While interval training is clearly a potent stimulus for physiological remodelling in humans, the integrative response to this type of exercise warrants further attention, especially in comparison to traditional endurance training. © 2016 The Authors. The Journal of Physiology © 2016 The Physiological Society.
Kumyaito, Nattapon; Yupapin, Preecha; Tamee, Kreangsak
An effective training plan is an important factor in sports training to enhance athletic performance. A poorly considered training plan may result in injury to the athlete, and overtraining. Good training plans normally require expert input, which may have a cost too great for many athletes, particularly amateur athletes. The objectives of this research were to create a practical cycling training plan that substantially improves athletic performance while satisfying essential physiological constraints. Adaptive Particle Swarm Optimization using ɛ-constraint methods were used to formulate such a plan and simulate the likely performance outcomes. The physiological constraints considered in this study were monotony, chronic training load ramp rate and daily training impulse. A comparison of results from our simulations against a training plan from British Cycling, which we used as our standard, showed that our training plan outperformed the benchmark in terms of both athletic performance and satisfying all physiological constraints.
M. Khodzhiev; N.F. Izmerov; I.V. Bukhtiyarov
The paper focuses on the results of physiological research performed on 9 occupational groups of migrants who came to Moscow region of Russia from southern Central Asia republics. We studied adaptation processes characteristics in labor migrants as per cardiovascular system parameters and neuromuscular apparatus parameters. We revealed peculiarities in heart rate variability (changes in stress index S1, values of AMo, VLF spectrum power, PARS parameter) in migrants de-pending on neuro-emotion...
Huey, Raymond B.; Kearney, Michael R.; Krockenberger, Andrew; Holtum, Joseph A. M.; Jess, Mellissa; Williams, Stephen E.
A recently developed integrative framework proposes that the vulnerability of a species to environmental change depends on the species' exposure and sensitivity to environmental change, its resilience to perturbations and its potential to adapt to change. These vulnerability criteria require behavioural, physiological and genetic data. With this information in hand, biologists can predict organisms most at risk from environmental change. Biologists and managers can then target organisms and habitats most at risk. Unfortunately, the required data (e.g. optimal physiological temperatures) are rarely available. Here, we evaluate the reliability of potential proxies (e.g. critical temperatures) that are often available for some groups. Several proxies for ectotherms are promising, but analogous ones for endotherms are lacking. We also develop a simple graphical model of how behavioural thermoregulation, acclimation and adaptation may interact to influence vulnerability over time. After considering this model together with the proxies available for physiological sensitivity to climate change, we conclude that ectotherms sharing vulnerability traits seem concentrated in lowland tropical forests. Their vulnerability may be exacerbated by negative biotic interactions. Whether tropical forest (or other) species can adapt to warming environments is unclear, as genetic and selective data are scant. Nevertheless, the prospects for tropical forest ectotherms appear grim. PMID:22566674
Full Text Available Prolactin is one of the most versatile hormones known. It is considered an adaptive hormone due to the key roles it plays in the modulation of the stress response and during pregnancy and lactation. Within the brain, prolactin acts as a neuropeptide to promote physiological responses related to reproduction, stress adaptation, neurogenesis, and neuroprotection. The action of prolactin on the nervous system contributes to the wide array of changes that occur in the female brain during pregnancy and result in the attenuation of the hypothalamic pituitary adrenal axis. Together, all these changes promote behavioral and physiological adaptations of the new mother to enable reproductive success. Brain adaptations driven by prolactin are also important for the regulation of maternal emotionality and wellbeing Prolactin also affects the male brain during the stress response but its effects have been less studied. Prolactin regulates neurogenesis both in the subventricular zone and in the hippocampus. Therefore, alterations in the prolactin system due to stress, or exposure to substances that reduce neurogenesis or other conditions, could contribute to maladaptive responses and pathological behavioral outcomes. Here we review the prolactin system and the role it plays in the modulation of stress response and emotion regulation. We discuss the effects of prolactin on neurogenesis and neuroprotection, the putative neuronal mechanisms underlying these effects, and their contribution to the onset of psychopathological states like depression.
André, G.; Engel, B.; Berentsen, P.B.M.; Oude Lansink, A.G.J.M.
Automated feeding and milking of dairy cows enables the application of individual cow settings for concentrate supply and milking frequency. Currently, general settings are used, based on knowledge about energy and nutrient requirements in relation to milk production at the group level. Individual
Farney, Jaymelynn K.; Mamedova, Laman K.; Coetzee, Johann F.; KuKanich, Butch; Sordillo, Lorraine M.; Stoakes, Sara K.; Minton, J. Ernest; Hollis, Larry C.; Bradford, Barry J.
Adapting to the lactating state requires metabolic adjustments in multiple tissues, especially in the dairy cow, which must meet glucose demands that can exceed 5 kg/day in the face of negligible gastrointestinal glucose absorption. These challenges are met through the process of homeorhesis, the alteration of metabolic setpoints to adapt to a shift in physiological state. To investigate the role of inflammation-associated pathways in these homeorhetic adaptations, we treated cows with the no...
Carlos E. Oltramari
Full Text Available Twenty-four dairy cows, with daily average milk production of 18.1 kg, were fed diets containing different selenium (Se sources. The purpose of this paper is to evaluate the effects of such diets on milk production and quality, on the occurrence of mastitis, and on physiological variables. During the experimental period (124 days, all the cows received the same diet: a total mixed feed with 0.278 mg.kg- 1 DM of selenium. In the inorganic Se treatment, the selenium source was sodium selenite and in the organic Se treatment the source was selenium yeast (Saccharomyces cerevisiae CNCM I-3060. There were no significant differences in milk yield or in Se concentration in the milk. No significant differences between the treatments were observed in protein, lactose, solids-not-fat andpercentage of total solids. The animals subjected to the organic Se treatment presented higher (P=0.013 percentage of milk fat and lower (P=0.014 somatic cell count (SCC than those subjected to the inorganic Se treatment. There was no significant difference in subclinical mastitisas determined by the California Mastitis Test (CMT between the treatments. However, both Se sources reduced the incidence of mastitis (subclinical positive mastitis and strongly positive mastitis between the pre-experimental and experimental phases. There was no significant difference in rectal temperature (RT between the treatments. Respiratory frequency (RF was lower (P=0.027 in the inorganic treatment than in the organic one, whereas haircoat temperature (HT was lower (P=0.007 in the organic treatment than in the inorganic one.
Daramola, J O; Adeloye, A A
West African Dwarf (WAD) goats are widely distributed in the subhumid and humid zones of Africa but are particularly associated with less favourable environments. Adaptive features such as feeding behaviour, efficiency of feed use and disease tolerance enable WAD goats to thrive on natural resources left untouched by other domestic ruminants. In marginal environments this goat remains the only domestic species that is able to survive. Among its physiological features small body size and low metabolic requirements are important traits that enable the animal to minimize its requirements in area or season where food sources are limited in quality and quantity. Specialized feeding behaviour and an efficient digestive system enable the animal to maximize food intake. Coat colour plays an important role in the evolved adaptation of this goat type. Reproductive fitness as manifested by prolific breeding is a major factor of adaptation. Defence mechanisms against infectious agents enable this type to thrive well in the hot humid tropics. The mechanisms involved in the regulation of these physiological functions of WAD goat are discussed. An understanding of these mechanisms could result in the development of improved techniques for enhancing goat productivity in humid environments.
Bianchini, Adalto; Lauer, Mariana Machado; Nery, Luiz Eduardo Maia; Colares, Elton Pinto; Monserrat, José María; Dos Santos Filho, Euclydes Antônio
Neohelice granulata (Chasmagnathus granulatus) is an intertidal crab species living in salt marshes from estuaries and lagoons along the Atlantic coast of South America. It is a key species in these environments because it is responsible for energy transfer from producers to consumers. In order to deal with the extremely marked environmental salinity changes occurring in salt marshes, N. granulata shows important and interesting structural, biochemical, and physiological adaptations at the gills level. These adaptations characterize this crab as a euryhaline species, tolerating environmental salinities ranging from very diluted media to concentrated seawater. These characteristics had led to its use as an animal model to study estuarine adaptations in crustaceans. Therefore, the present review focuses on the influence of environmental salinity on N. granulata responses at the ecological, organismic and molecular levels. Aspects covered include salinity tolerance, osmo- and ionoregulatory patterns, morphological and structural adaptations at the gills, and mechanisms of ion transport and their regulation at the gills level during environmental salinity acclimation. Finally, this review compiles information on the effects of some environmental pollutants on iono- and osmoregulatory adaptations showed by N. granulata.
He, Z.; Zhou, A.; Baidoo, E.; He, Q.; Joachimiak, M. P.; Benke, P.; Phan, R.; Mukhopadhyay, A.; Hemme, C.L.; Huang, K.; Alm, E.J.; Fields, M.W.; Wall, J.; Stahl, D.; Hazen, T.C.; Keasling, J.D.; Arkin, A.P.; Zhou, J.
The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by physiological, global transcriptional, and metabolite analyses. The growth of D. vulgaris was inhibited by high levels of NaCl, and the growth inhibition could be relieved by the addition of exogenous amino acids (e.g., glutamate, alanine, tryptophan) or yeast extract. Salt adaptation induced the expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). Genes involved in carbon metabolism, cell motility, and phage structures were repressed. Comparison of transcriptomic profiles of D. vulgaris responses to salt adaptation with those of salt shock (short-term NaCl exposure) showed some similarity as well as a significant difference. Metabolite assays showed that glutamate and alanine were accumulated under salt adaptation, suggesting that they may be used as osmoprotectants in D. vulgaris. A conceptual model is proposed to link the observed results to currently available knowledge for further understanding the mechanisms of D. vulgaris adaptation to elevated NaCl.
Zhang, Y; Sun, J W; Rolfe, P
The non-invasive measurement of cerebral functional haemodynamics using near-infrared spectroscopy (NIRS) instruments is often affected by physiological interference. The suppression of this interference is crucial for reliable recovery of brain activity measurements because it can significantly affect the signal quality. In this study, we present a recursive least-squares (RLS) algorithm for adaptive filtering to reduce the magnitude of the physiological interference component. To evaluate it, we implemented Monte Carlo simulations based on a five-layer slab model of a human adult head with a multidistance source–detector arrangement, of a short pair and a long pair, for NIRS measurement. We derived measurements by adopting different interoptode distances, which is relevant to the process of optimizing the NIRS probe configuration. Both RLS and least mean squares (LMS) algorithms were used to attempt the removal of physiological interference. The results suggest that the RLS algorithm is more capable of minimizing the effect of physiological interference due to its advantages of faster convergence and smaller mean squared error (MSE). The influence of superficial layer thickness on the performance of the RLS algorithm was also investigated. We found that the near-detector position is an important variable in minimizing the MSE and a short source–detector separation less than 9 mm is robust to superficial layer thickness variation. (paper)
van Oppen Madeleine JH
Full Text Available Abstract Background Divergent natural selection across environmental gradients has been acknowledged as a major driver of population and species divergence, however its role in the diversification of scleractinian corals remains poorly understood. Recently, it was demonstrated that the brooding coral Seriatopora hystrix and its algal endosymbionts (Symbiodinium are genetically partitioned across reef environments (0-30 m on the far northern Great Barrier Reef. Here, we explore the potential mechanisms underlying this differentiation and assess the stability of host-symbiont associations through a reciprocal transplantation experiment across habitats ('Back Reef', 'Upper Slope' and 'Deep Slope', in combination with molecular (mtDNA and ITS2-DGGE and photo-physiological analyses (respirometry and HPLC. Results The highest survival rates were observed for native transplants (measured 14 months after transplantation, indicating differential selective pressures between habitats. Host-symbiont assemblages remained stable during the experimental duration, demonstrating that the ability to "shuffle" or "switch" symbionts is restricted in S. hystrix. Photo-physiological differences were observed between transplants originating from the shallow and deep habitats, with indirect evidence of an increased heterotrophic capacity in native deep-water transplants (from the 'Deep Slope' habitat. Similar photo-acclimatisation potential was observed between transplants originating from the two shallow habitats ('Back Reef' and 'Upper Slope', highlighting that their genetic segregation over depth may be due to other, non-photo-physiological traits under selection. Conclusions This study confirms that the observed habitat partitioning of S. hystrix (and associated Symbiodinium is reflective of adaptive divergence along a depth gradient. Gene flow appears to be reduced due to divergent selection, highlighting the potential role of ecological mechanisms, in addition to
David J McKenzie
Full Text Available In watersheds of equatorial West Africa, monophyletic groups of killifish species (genus Aphyosemion occur in discrete altitudinal ranges, low altitude species (LA, sea level to ∼350 m or high altitude species (HA, 350 to 900 m. We investigated the hypothesis that local adaptation to altitude by the LA and HA species would be revealed as divergent effects of temperature on their physiological energetics. Two species from each group (mass ∼350 mg were acclimated to 19, 25 and 28°C, with 19 and 28°C estimated to be outside the thermal envelope for LA or HA, respectively, in the wild. Wild-caught animals (F0 generation were compared with animals raised in captivity at 25°C (F1 generation to investigate the contribution of adaptation versus plasticity. Temperature significantly increased routine metabolic rate in all groups and generations. However, LA and HA species differed in the effects of temperature on their ability to process a meal. At 25°C, the specific dynamic action (SDA response was completed within 8 h in all groups, but acclimation to temperatures beyond the thermal envelope caused profound declines in SDA performance. At 19°C, the LA required ∼14 h to complete the SDA, whereas the HA required only ∼7 h. The opposite effect was observed at 28°C. This effect was evident in both F0 and F1. Reaction norms for effects of temperature on SDA therefore revealed a trade-off, with superior performance at warmer temperatures by LA being associated with inferior performance at cooler temperatures, and vice-versa in HA. The data indicate that divergent physiological responses to temperature in the LA and HA species reflect local adaptation to the thermal regime in their habitat, and that local adaptation to one thermal environment trades off against performance in another.
Edifizi, Diletta; Schumacher, Björn
DNA damage causally contributes to aging and age-related diseases. The declining functioning of tissues and organs during aging can lead to the increased risk of succumbing to aging-associated diseases. Congenital syndromes that are caused by heritable mutations in DNA repair pathways lead to cancer susceptibility and accelerated aging, thus underlining the importance of genome maintenance for withstanding aging. High-throughput mass-spectrometry-based approaches have recently contributed to identifying signalling response networks and gaining a more comprehensive understanding of the physiological adaptations occurring upon unrepaired DNA damage. The insulin-like signalling pathway has been implicated in a DNA damage response (DDR) network that includes epidermal growth factor (EGF)-, AMP-activated protein kinases (AMPK)- and the target of rapamycin (TOR)-like signalling pathways, which are known regulators of growth, metabolism, and stress responses. The same pathways, together with the autophagy-mediated proteostatic response and the decline in energy metabolism have also been found to be similarly regulated during natural aging, suggesting striking parallels in the physiological adaptation upon persistent DNA damage due to DNA repair defects and long-term low-level DNA damage accumulation occurring during natural aging. These insights will be an important starting point to study the interplay between signalling networks involved in progeroid syndromes that are caused by DNA repair deficiencies and to gain new understanding of the consequences of DNA damage in the aging process.
Full Text Available DNA damage causally contributes to aging and age-related diseases. The declining functioning of tissues and organs during aging can lead to the increased risk of succumbing to aging-associated diseases. Congenital syndromes that are caused by heritable mutations in DNA repair pathways lead to cancer susceptibility and accelerated aging, thus underlining the importance of genome maintenance for withstanding aging. High-throughput mass-spectrometry-based approaches have recently contributed to identifying signalling response networks and gaining a more comprehensive understanding of the physiological adaptations occurring upon unrepaired DNA damage. The insulin-like signalling pathway has been implicated in a DNA damage response (DDR network that includes epidermal growth factor (EGF-, AMP-activated protein kinases (AMPK- and the target of rapamycin (TOR-like signalling pathways, which are known regulators of growth, metabolism, and stress responses. The same pathways, together with the autophagy-mediated proteostatic response and the decline in energy metabolism have also been found to be similarly regulated during natural aging, suggesting striking parallels in the physiological adaptation upon persistent DNA damage due to DNA repair defects and long-term low-level DNA damage accumulation occurring during natural aging. These insights will be an important starting point to study the interplay between signalling networks involved in progeroid syndromes that are caused by DNA repair deficiencies and to gain new understanding of the consequences of DNA damage in the aging process.
Smith, R L
Results are reviewed concerning some effects, at a units's characteristic frequency, of a short-term conditioning stimulus on the responses to perstimulatory and poststimulatory test tones. A phenomenological equation is developed from the poststimulatory results and shown to be consistent with the perstimulatory results. According to the results and equation, the response to a test tone equals the unconditioned or unadapted response minus the decrement produced by adaptation to the conditioning tone. Furthermore, the decrement is proportional to the driven response to the conditioning tone and does not depend on sound intensity per se. The equation has a simple interpretation in terms of two processes in cascade--a static saturating nonlinearity followed by additive adaptation. Results are presented to show that this functional model is sufficient to account for the "physiological masking" produced by wide-band backgrounds. According to this interpretation, a sufficiently intense background produces saturation. Consequently, a superimposed test tone cause no change in response. In addition, when the onset of the background precedes the onset of the test tone, the total firing rate is reduced by adaptation. Evidence is reviewed concerning the possible correspondence between the variables in the model and intracellular events in the auditory periphery.
Piechotta, Marion; Holzhausen, Lars; Araujo, Marcelo Gil; Heppelmann, Maike; Sipka, Anja; Pfarrer, Chistiane; Schuberth, Hans-Joachim; Bollwein, Heinrich
Cows with different Insulin-like Growth Factor-I (IGF-I) concentrations showed comparable expression levels of hepatic growth hormone receptor (GHR). Suppressor of cytokine signaling 2 (SOCS2), could be responsible for additional inhibition of the GHR signal cascade. The aims were to monitor cows with high or low antepartal IGF-I concentrations (IGF-I(high) or IGF-I(low)), evaluate the interrelationships of endocrine endpoints, and measure hepatic SOCS2 expression. Dairy cows (n = 20) were selected (240 to 254 days after artificial insemination (AI)). Blood samples were drawn daily (day -17 until calving) and IGF-I, GH, insulin, thyroid hormones, estradiol, and progesterone concentrations were measured. Liver biopsies were taken (day 264 ± 1 after AI and postpartum) to measure mRNA expression (IGF-I, IGFBP-2, IGFBP-3, IGFBP-4, acid labile subunit (ALS), SOCS2, deiodinase1, GHR1A). IGF-I concentrations in the two groups were different (p 0.05). Thyroxine levels and ALS expression were higher in the IGF-I(high) cows compared to IGF-I(low) cows. Estradiol concentration tended to be greater in the IGF-I(low) group (p = 0.06). It was hypothesized that low IGF-I levels are associated with enhanced SOCS2 expression although this could not be decisively confirmed by the present study.
Holzhausen, Lars; Araujo, Marcelo Gil; Heppelmann, Maike; Sipka, Anja; Pfarrer, Chistiane; Schuberth, Hans-Joachim; Bollwein, Heinrich
Cows with different Insulin-like Growth Factor-I (IGF-I) concentrations showed comparable expression levels of hepatic growth hormone receptor (GHR). Suppressor of cytokine signaling 2 (SOCS2), could be responsible for additional inhibition of the GHR signal cascade. The aims were to monitor cows with high or low antepartal IGF-I concentrations (IGF-Ihigh or IGF-Ilow), evaluate the interrelationships of endocrine endpoints, and measure hepatic SOCS2 expression. Dairy cows (n = 20) were selected (240 to 254 days after artificial insemination (AI)). Blood samples were drawn daily (day -17 until calving) and IGF-I, GH, insulin, thyroid hormones, estradiol, and progesterone concentrations were measured. Liver biopsies were taken (day 264 ± 1 after AI and postpartum) to measure mRNA expression (IGF-I, IGFBP-2, IGFBP-3, IGFBP-4, acid labile subunit (ALS), SOCS2, deiodinase1, GHR1A). IGF-I concentrations in the two groups were different (p 0.05). Thyroxine levels and ALS expression were higher in the IGF-Ihigh cows compared to IGF-Ilow cows. Estradiol concentration tended to be greater in the IGF-Ilow group (p = 0.06). It was hypothesized that low IGF-I levels are associated with enhanced SOCS2 expression although this could not be decisively confirmed by the present study. PMID:24962413
Katiyatiya, C. L. F.; Muchenje, V.; Mushunje, A.
Seasonal variations in hair length, tick loads, cortisol levels, haematological parameters (HP) and temperature humidity index (THI) in Nguni cows of different colours raised in two low-input farms, and a commercial stud was determined. The sites were chosen based on their production systems, climatic characteristics and geographical locations. Zazulwana and Komga are low-input, humid-coastal areas, while Honeydale is a high-input, dry-inland Nguni stud farm. A total of 103 cows, grouped according to parity, location and coat colour, were used in the study. The effects of location, coat colour, hair length and season were used to determine tick loads on different body parts, cortisol levels and HP in blood from Nguni cows. Highest tick loads were recorded under the tail and the lowest on the head of each of the animals ( P cows recorded the highest tick loads under the tails of all the cows used in the study from the three farms ( P cows with long hairs. Hair lengths were longest during the winter season in the coastal areas of Zazulwana and Honeydale ( P cows had significantly longer ( P heat stress in Nguni cows.
Full Text Available Shift work has become an integral part of our life with almost 20% of the population being involved in different shift schedules in developed countries. However, the atypical work times, especially the night shifts, are associated with reduced quality and quantity of sleep that leads to increase of sleepiness often culminating in accidents. It has been demonstrated that shift workers' sleepiness can be improved by a proper scheduling of light exposure and optimizing shifts timing. Here, an integrated physiologically-based model of sleep-wake cycles is used to predict adaptation to shift work in different light conditions and for different shift start times for a schedule of four consecutive days of work. The integrated model combines a model of the ascending arousal system in the brain that controls the sleep-wake switch and a human circadian pacemaker model. To validate the application of the integrated model and demonstrate its utility, its dynamics are adjusted to achieve a fit to published experimental results showing adaptation of night shift workers (n = 8 in conditions of either bright or regular lighting. Further, the model is used to predict the shift workers' adaptation to the same shift schedule, but for conditions not considered in the experiment. The model demonstrates that the intensity of shift light can be reduced fourfold from that used in the experiment and still produce good adaptation to night work. The model predicts that sleepiness of the workers during night shifts on a protocol with either bright or regular lighting can be significantly improved by starting the shift earlier in the night, e.g.; at 21:00 instead of 00:00. Finally, the study predicts that people of the same chronotype, i.e. with identical sleep times in normal conditions, can have drastically different responses to shift work depending on their intrinsic circadian and homeostatic parameters.
Postnova, Svetlana; Robinson, Peter A; Postnov, Dmitry D
Shift work has become an integral part of our life with almost 20% of the population being involved in different shift schedules in developed countries. However, the atypical work times, especially the night shifts, are associated with reduced quality and quantity of sleep that leads to increase of sleepiness often culminating in accidents. It has been demonstrated that shift workers' sleepiness can be improved by a proper scheduling of light exposure and optimizing shifts timing. Here, an integrated physiologically-based model of sleep-wake cycles is used to predict adaptation to shift work in different light conditions and for different shift start times for a schedule of four consecutive days of work. The integrated model combines a model of the ascending arousal system in the brain that controls the sleep-wake switch and a human circadian pacemaker model. To validate the application of the integrated model and demonstrate its utility, its dynamics are adjusted to achieve a fit to published experimental results showing adaptation of night shift workers (n = 8) in conditions of either bright or regular lighting. Further, the model is used to predict the shift workers' adaptation to the same shift schedule, but for conditions not considered in the experiment. The model demonstrates that the intensity of shift light can be reduced fourfold from that used in the experiment and still produce good adaptation to night work. The model predicts that sleepiness of the workers during night shifts on a protocol with either bright or regular lighting can be significantly improved by starting the shift earlier in the night, e.g.; at 21:00 instead of 00:00. Finally, the study predicts that people of the same chronotype, i.e. with identical sleep times in normal conditions, can have drastically different responses to shift work depending on their intrinsic circadian and homeostatic parameters.
Postnova, Svetlana; Robinson, Peter A.; Postnov, Dmitry D.
Shift work has become an integral part of our life with almost 20% of the population being involved in different shift schedules in developed countries. However, the atypical work times, especially the night shifts, are associated with reduced quality and quantity of sleep that leads to increase of sleepiness often culminating in accidents. It has been demonstrated that shift workers’ sleepiness can be improved by a proper scheduling of light exposure and optimizing shifts timing. Here, an integrated physiologically-based model of sleep-wake cycles is used to predict adaptation to shift work in different light conditions and for different shift start times for a schedule of four consecutive days of work. The integrated model combines a model of the ascending arousal system in the brain that controls the sleep-wake switch and a human circadian pacemaker model. To validate the application of the integrated model and demonstrate its utility, its dynamics are adjusted to achieve a fit to published experimental results showing adaptation of night shift workers (n = 8) in conditions of either bright or regular lighting. Further, the model is used to predict the shift workers’ adaptation to the same shift schedule, but for conditions not considered in the experiment. The model demonstrates that the intensity of shift light can be reduced fourfold from that used in the experiment and still produce good adaptation to night work. The model predicts that sleepiness of the workers during night shifts on a protocol with either bright or regular lighting can be significantly improved by starting the shift earlier in the night, e.g.; at 21∶00 instead of 00∶00. Finally, the study predicts that people of the same chronotype, i.e. with identical sleep times in normal conditions, can have drastically different responses to shift work depending on their intrinsic circadian and homeostatic parameters. PMID:23308206
Full Text Available The paper focuses on the results of physiological research performed on 9 occupational groups of migrants who came to Moscow region of Russia from southern Central Asia republics. We studied adaptation processes characteristics in labor migrants as per cardiovascular system parameters and neuromuscular apparatus parameters. We revealed peculiarities in heart rate variability (changes in stress index S1, values of AMo, VLF spectrum power, PARS parameter in migrants de-pending on neuro-emotional nature of working activity and muscular loads intensity. We detected apparent decrease in dynamometric parameters of endurance and maximum working capacity of arms muscles and backbones and legs muscles by the end of their working day. We created a procedure to determine an adaptation process stage under combined effects ex-erted by labor physical hardness and neuro-emotional intensity; unsatisfactory adaptation increases health disorders risks. We showed that long-term and intense impacts by factors determining labor process hardness caused occupational diseases of musculoskeletal system and peripheral nervous system. We revealed that as labor intensity grew working stress in a body also increased and it could transform into overstrain and occupational diseases evolvement (primary hypertension, ischemic heart diseases, and neurotic disorders. A distinctive feature is that men suffer from cardiovascular system pathologies more frequently while women tend to suffer from nervous system pathologies. In this relation, we should make separate predictions of occupational pathology probability depending on labor intensity level for males and females. Adaptation processes optimization should include organization of rational work and rest regime, healthy lifestyle for-mation, improvements in medical aid for migrants.
Habeeb, A.A.M.; Marai, I.F.M.; Gabr, H.; Farghaly, H.M.
A number of 20 of each of healthy lactating friesian and holstein cows similar age and body weight, non-pregnant at the 3 rd lactation season, 80-100 days postpartum, were used in the present study. The investigation was carried out in two farms at the same time during the hot summer season of egypt and lasted 8 weeks. The first farm was in damietta (North east of the nile delta, 31 degree 40 N) including 20 friesian cows and the second one was in Fakous (East of the Nile delta 30 degree 40 N) including 20 holstein cows. In each of the two farms, 10 cows were newly imported and other 10 ones were born in egypt. The results showed that the newly imported cows were significantly (P <0.01) higher than the locally born cows in average daily milk yield, as well as, milk production in both friesian and holstein strains at the same time, the newly imported cows were significantly (P < 0.01 or 0.05) lower than the locally born in T4, T3, urea-N, blood picture values and GPT enzyme activity in the two strains of cattle, while the locally born cows showed significantly (P < 0.01 or 0.05) lower values in rectal temperature and respiration rate, as well as, Got and ALK-P enzymes activities than in the newly imported cows either in friesian or holstein cattle. Holstein cows surpassed friesian ones (P < 0.01 or 0.05) in milk production, thyroid hormones content, cholesterol, haemoglobin, packed cell volume and erythrocyte count values. The opposite was true in serum total protein, urea-N, creatinine concentrations, leucocyte counts, Got, GPT and ALK-P enzymes activities
Becker, Karsten; Bierbaum, Gabriele; von Eiff, Christof; Engelmann, Susanne; Götz, Friedrich; Hacker, Jörg; Hecker, Michael; Peters, Georg; Rosenstein, Ralf; Ziebuhr, Wilma
Staphylococcus aureus as well as coagulase-negative staphylococci are medically highly important pathogens characterized by an increasing resistance rate toward many antibiotics. Although normally being skin and mucosa commensals, some staphylococcal species and strains have the capacity to cause a wide range of infectious diseases. Many of these infections affect immunocompromised patients in hospitals. However, community-acquired staphylococcal infections due to resistant strains are also currently on the rise. In the light of this development, there is an urgent need for novel anti-staphylococcal therapeutic and prevention strategies for which a better understanding of the physiology of these bacteria is an essential prerequisite. Within the past years, staphylococci have been in the focus of genomic research, resulting in the determination and publication of a range of full-genome sequences of different staphylococcal species and strains which provided the basis for the design and application of DNA microarrays and other genomic tools. Here we summarize the results of the project group 'Staphylococci' within the research network 'Pathogenomics' giving new insights into the genome structure, molecular epidemiology, physiology, and genetic adaptation of both S. aureus and coagulase-negative staphylococci.
Fléchard, Maud; Gilot, Philippe
We have referenced and described Streptococcus agalactiae transposable elements encoding DDE transposases. These elements belonged to nine families of insertion sequences (ISs) and to a family of conjugative transposons (TnGBSs). An overview of the physiological impact of the insertion of all these elements is provided. DDE-transposable elements affect S. agalactiae in a number of aspects of its capability to adapt to various environments and modulate the expression of several virulence genes, the scpB-lmB genomic region and the genes involved in capsule expression and haemolysin transport being the targets of several different mobile elements. The referenced mobile elements modify S. agalactiae behaviour by transferring new gene(s) to its genome, by modifying the expression of neighbouring genes at the integration site or by promoting genomic rearrangements. Transposition of some of these elements occurs in vivo, suggesting that by dynamically regulating some adaptation and/or virulence genes, they improve the ability of S. agalactiae to reach different niches within its host and ensure the 'success' of the infectious process. © 2014 The Authors.
Sawka, Michael N; Leon, Lisa R; Montain, Scott J; Sonna, Larry A
This article emphasizes significant recent advances regarding heat stress and its impact on exercise performance, adaptations, fluid electrolyte imbalances, and pathophysiology. During exercise-heat stress, the physiological burden of supporting high skin blood flow and high sweating rates can impose considerable cardiovascular strain and initiate a cascade of pathophysiological events leading to heat stroke. We examine the association between heat stress, particularly high skin temperature, on diminishing cardiovascular/aerobic reserves as well as increasing relative intensity and perceptual cues that degrade aerobic exercise performance. We discuss novel systemic (heat acclimation) and cellular (acquired thermal tolerance) adaptations that improve performance in hot and temperate environments and protect organs from heat stroke as well as other dissimilar stresses. We delineate how heat stroke evolves from gut underperfusion/ischemia causing endotoxin release or the release of mitochondrial DNA fragments in response to cell necrosis, to mediate a systemic inflammatory syndrome inducing coagulopathies, immune dysfunction, cytokine modulation, and multiorgan damage and failure. We discuss how an inflammatory response that induces simultaneous fever and/or prior exposure to a pathogen (e.g., viral infection) that deactivates molecular protective mechanisms interacts synergistically with the hyperthermia of exercise to perhaps explain heat stroke cases reported in low-risk populations performing routine activities. Importantly, we question the "traditional" notion that high core temperature is the critical mediator of exercise performance degradation and heat stroke. Published 2011. This article is a U.S. Government work and is in the public domain in the USA.
Soberon, M A; Cherney, J H; Liu, R H; Ross, D A; Cherney, D J R
Ferulic acid (FRA), a phenolic compound with antioxidant and anticancer activities, naturally occurs in plants as a lignin precursor. Many veins of research have been devoted to releasing FRA from the lignin complex to improve digestibility of ruminant feeds. Thus, the objective of this research was to investigate the transfer of a given dosage of the free form of FRA into the milk of dairy cattle. Six mid- to late-lactation Holstein cows at the Cornell Research Farm (Harford, NY) were given 14-d adaptation to diet and stall position. Ad libitum access to a total mixed ration based on haylage and maize silage (31.1% neutral detergent fiber containing 5.52 mg of FRA/g) was provided during the study. A crossover design was implemented so that each cow alternated weekly between FRA-dosed and control. On d 1, jugular cannulas and urine catheters were placed in all cows. On d 2, FRA-dosed cows received a single dosage of 150 g of pure FRA powder at 0830 h via their fistula (n=4) or a balling gun for nonfistulated cows (n=2). Plasma, urine, feces, feed, orts, milk, and rumen fluid were sampled intensively for the next 36 h and analyzed for FRA concentration. On d 8, the cows crossed over and the experiment was repeated. When compared with the control, FRA administration did not have an effect on dry matter intake, milk yield, milk fat yield, milk protein yield, somatic cell count, or neutral detergent fiber content of orts and feces. The concentration of FRA in the feces did not change as a result of FRA dosage. As expected, FRA concentration increased dramatically upon FRA dosage and decreased over time until returning to basal levels in rumen fluid (4 h after dosage), plasma (5.5 h after dosage), urine (10 h after dosage), and milk (14 h after dosage). Baseline values for FRA in urine and rumen fluid were variable among cows and had an effect on FRA concentration in FRA-dosed cows. From this study, it is observed that orally ingested FRA can be transported into the
Becker, B. A.; Johnson, H. D.; Li, R.; Collier, R. J.
A study was conducted to evaluate the effect of bovine somatotropin (BST) supplementation in twelve lactating dairy cows maintained in cold environmental conditions. Six cows were injected daily with 25 mg of BST; the other six were injected with a control vehicle. Cows were maintained under standard dairy management during mid-winter for 30 days. Milk production was recorded twice daily, and blood samples were taken weekly. Animals were then transferred to environmentally controlled chambers and exposed to cycling thermoneutral (15° to 20° C) and cycling cold (-5° to +5° C) temperatures for 10 days in a split-reversal design. Milk production, feed and water intake, body weights and rectal temperatures were monitored. Blood samples were taken on days 1, 3, 5, 8 and 10 of each period and analyzed for plasma triiodothyronine (T3), thyroxine (T4), cortisol, insulin and prolactin. Under farm conditions, BST-treated cows produced 11% more milk than control-treated cows and in environmentally controlled chambers produced 17.4% more milk. No differences due to BST in feed or water intake, body weights or rectal temperatures were found under laboratory conditions. Plasma T3 and insulin increased due to BST treatment while no effect was found on cortisol, prolactin or T4. The results showed that the benefits of BST supplementation in lactating dairy cows were achieved under cold environmental conditions.
Full Text Available Experimentation on the International Space Station has reached the stage where repeated and nuanced transcriptome studies are beginning to illuminate the structural and metabolic differences between plants grown in space compared to plants on the Earth. Genes that are important in establishing the spaceflight responses are being identified, their roles in spaceflight physiological adaptation are increasingly understood, and the fact that different genotypes adapt differently is recognized. However, the basic question of whether these spaceflight responses are actually required for survival has yet to be posed, and the fundamental notion that spaceflight responses may be non-adaptive has yet to be explored. Therefore the experiments presented here were designed to ask if portions of the plant spaceflight response can be genetically removed without causing loss of spaceflight survival and without causing increased stress responses. The CARA experiment compared the spaceflight transcriptome responses in the root tips of two Arabidopsis ecotypes, Col-0 and WS, as well as that of a PhyD mutant of Col-0. When grown with the ambient light of the ISS, phyD plants displayed a significantly reduced spaceflight transcriptome response compared to Col-0, suggesting that altering the activity of a single gene can actually improve spaceflight adaptation by reducing the transcriptome cost of physiological adaptation. The WS genotype showed an even simpler spaceflight transcriptome response in the ambient light of the ISS, more broadly indicating that the plant genotype can be manipulated to reduce the cost of spaceflight adaptation, as measured by transcriptional response. These differential genotypic responses suggest that genetic manipulation could further reduce, or perhaps eliminate the metabolic cost of spaceflight adaptation. When plants were germinated and then left in the dark on the ISS, the WS genotype actually mounted a larger transcriptome response
Christian O. Chukwuka
Full Text Available The survival of land snails in an adverse environmental condition depends on the integral physiological, morphological and behavioural adaptations. These adaptations are essential in understanding the species-specific habitat requirements and in predicting their environmental responses. In this study, the monthly and the periodic patterns of eco-physiological adaptation of land snail, Achatina achatina in Nsukka tropical agro-ecosystem were assessed from December 2012 to July 2013. Standard methods were employed in sampling the land snail and determination of the water content, biochemical fuel reserves and enzyme concentrations of the samples. The present results showed that lipids were high at the beginning of aestivation and depleted as the aestivation progressed. Glycogen was significantly low throughout the aestivation months (December–March and increased in the active months (April–July. Protein content recorded a definite pattern all through the months studied. Catabolism of lactate and a decrease in activity of LDH during aestivation and substantial increase upon activation were observed. Data showed that transaminase and aspartate enzymes depleted during the aestivation months indicating that the snails may have developed potential cell injury due to oxidative stress and thermal heat. A disassociation between the physiological responses and climatic data was recorded. The physiological adaptation of A. achatina ensures regular adjustment under extreme conditions and compensates for its metabolic regulation in the tropics. It is concluded that survival of A. achatina is not environmentally predicted; rather it depends on the species-specific inherent process in predicting responses for survival.
Sreeman, Sheshshayee M.; Vijayaraghavareddy, Preethi; Sreevathsa, Rohini; Rajendrareddy, Sowmya; Arakesh, Smitharani; Bharti, Pooja; Dharmappa, Prathibha; Soolanayakanahally, Raju
Burgeoning population growth, industrial demand and the predicted global climate change resulting in erratic monsoon rains are expected to severely limit fresh water availability for agriculture both in irrigated and rainfed ecosystems. In order to remain food and nutrient secure, agriculture research needs to focus on devising strategies to save water in irrigated conditions and to develop superior cultivars with improved water productivity to sustain yield under rainfed conditions. Recent opinions accruing in the scientific literature strongly favour the adoption of a “trait based” approach for increasing water productivity especially the traits associated with maintenance of positive tissue turgor and maintenance of increased carbon assimilation as the most relevant traits to improve crop growth rates under water limiting conditions and to enhance water productivity. The advent of several water saving agronomic practices notwithstanding, a genetic enhancement strategy of introgressing distinct physiological, morphological and cellular mechanisms on to a single elite genetic background is essential for achieving a comprehensive improvement in drought adaptation in crop plants. The significant progress made in genomics, though would provide the necessary impetus, a clear understanding of the “traits” to be introgressed is the most essential need of the hour. Water uptake by a better root architecture, water conservation by preventing unproductive transpiration is crucial for maintaining positive tissue water relations. Improved carbon assimilation associated with carboxylation capacity and mesophyll conductance is equally important in sustaining crop growth rates under water limited conditions. Besides these major traits, we summarized the available information in literature on classifying various drought adaptive traits. We provide evidences that water-use efficiency when introgressed with moderately higher transpiration, would significantly enhance
Sheshshayee M. Sreeman
Full Text Available Burgeoning population growth, industrial demand, and the predicted global climate change resulting in erratic monsoon rains are expected to severely limit fresh water availability for agriculture both in irrigated and rainfed ecosystems. In order to remain food and nutrient secure, agriculture research needs to focus on devising strategies to save water in irrigated conditions and to develop superior cultivars with improved water productivity to sustain yield under rainfed conditions. Recent opinions accruing in the scientific literature strongly favor the adoption of a “trait based” crop improvement approach for increasing water productivity. Traits associated with maintenance of positive tissue turgor and maintenance of increased carbon assimilation are regarded as most relevant to improve crop growth rates under water limiting conditions and to enhance water productivity. The advent of several water saving agronomic practices notwithstanding, a genetic enhancement strategy of introgressing distinct physiological, morphological, and cellular mechanisms on to a single elite genetic background is essential for achieving a comprehensive improvement in drought adaptation in crop plants. The significant progress made in genomics, though would provide the necessary impetus, a clear understanding of the “traits” to be introgressed is the most essential need of the hour. Water uptake by a better root architecture, water conservation by preventing unproductive transpiration are crucial for maintaining positive tissue water relations. Improved carbon assimilation associated with carboxylation capacity and mesophyll conductance is important in sustaining crop growth rates under water limited conditions. Besides these major traits, we summarize the available information in literature on classifying various drought adaptive traits. We provide evidences that Water-Use Efficiency when introgressed with moderately higher transpiration, would
Full Text Available Objective: To report on a new surgical technique to treat obesity -Digestive Adaptation - and to present its preliminary results.Method: The technique includes a vertical (sleeve gastrectomy,omentectomy and enterectomy maintaining the initial 150-cmportionof the jejunum and the final 150-cm-portion of the ileum.The three first obese patients operated on are described. Results:With a minimum follow-up of 6 months, all patients refer earlysatiety, are free of symptoms and have a BMI <31 Kg/m2.Conclusions: This procedure does not use prostheses and doesnot cause exclusion of gastrointestinal segments. It does notcreate subocclusions neither malabsorption nor blind endoscopicareas and above all, it causes no harm to important digestivefunctions. Conversely, it aims at moderate restriction with earlysatiety by distension, and at interfering in the neuroendocrineprofile, resulting in slow gastric emptying, early and prolongedsatiety, as well as positive changes in the metabolic profile. Basedon recent physiological data, the procedure aims at decreasingthe production of ghrelin, plasminogen activator inhibitor-1 (PAI-1and resistin, and at raising the levels of glucagon-like peptide-1(GLP-1. The patients operated on do not need nutritional supportor to take drugs because of the procedure, which is easy and safeto perform.
Mustonen, Anne-Mari; Pyykönen, Teija; Puukka, Matti; Asikainen, Juha; Hänninen, Sari; Mononen, Jaakko; Nieminen, Petteri
This study investigated the physiological adaptations to fasting using the farmed blue fox (Alopex lagopus) as a model for the endangered wild arctic fox. Sixteen blue foxes were fed throughout the winter and 32 blue foxes were fasted for 22 d in Nov-Dec 2002. Half of the fasted blue foxes were food-deprived again for 22 d in Jan-Feb 2003. The farmed blue fox lost weight at a slower rate (0.97-1.02% body mass d(-1)) than observed previously in the arctic fox, possibly due to its higher initial body fat content. The animals experienced occasional fasting-induced hypoglycaemia, but their locomotor activity was not affected. The plasma triacylglycerol and glycerol concentrations were elevated during phase II of fasting indicating stimulated lipolysis, probably induced by the high growth hormone concentrations. The total cholesterol, HDL- and LDL-cholesterol, urea, uric acid and total protein levels and the urea:creatinine ratio decreased during fasting. Although the plasma levels of some essential amino acids increased, the blue foxes did not enter phase III of starvation characterized by stimulated proteolysis during either of the 22-d fasting procedures. Instead of excessive protein catabolism, it is liver dysfunction, indicated by the increased plasma bilirubin levels and alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase activities, that may limit the duration of fasting in the species.
Fibach-Paldi, Sharon; Burdman, Saul; Okon, Yaacov
Azospirillum brasilense is a plant growth promoting rhizobacterium (PGPR) that is being increasingly used in agriculture in a commercial scale. Recent research has elucidated key properties of A. brasilense that contribute to its ability to adapt to the rhizosphere habitat and to promote plant growth. They include synthesis of the auxin indole-3-acetic acid, nitric oxide, carotenoids, and a range of cell surface components as well as the ability to undergo phenotypic variation. Storage and utilization of polybetahydroxyalkanoate polymers are important for the shelf life of the bacteria in production of inoculants, products containing bacterial cells in a suitable carrier for agricultural use. Azospirillum brasilense is able to fix nitrogen, but despite some controversy, as judging from most systems evaluated so far, contribution of fixed nitrogen by this bacterium does not seem to play a major role in plant growth promotion. In this review, we focus on recent advances in the understanding of physiological properties of A. brasilense that are important for rhizosphere performance and successful interactions with plant roots. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Bjerre-Harpøth, Vibeke; Friggens, N C; Thorup, Vivi Mørkøre
. Blood was collected daily and analyzed for metabolites: nonesterified fatty acids (NEFA), BHBA, glucose, plasma urea nitrogen, and insulin. The revised quantitative insulin sensitivity check index (RQUICKI) was calculated for each cow. Liver biopsies collected before and during restriction were analyzed...... for triglycerides, glycogen, phospholipids, glucose, and total lipid content. A generalized linear mixed model was used to determine the effect of stage of lactation on responses during restriction. Regression analyses were used to examine the effect of pre-restriction levels on changes during restriction. Similar...... decreases in milk yield among groups indicate that the capacity of individual responses is dependent on milk yield but the coping strategies used are dependent on stage of lactation. Milk yield was a better predictor of feed intake than DIM. Plasma glucose decreased for all cows, and cows in early lactation...
Iggo, A; Ogawa, H
1. A total of fifty-four mechanoreceptor afferent units with fast conducting axons in the tibial nerve innervating the glabrous skin of the hind leg were isolated in anaesthetized cats. 2. Twenty-six rapidly adapting units (RA), eighteen slowly adapting units (SA) and ten Pacinian corpuscle units (PC) were differentiated from each other mainly on the presence of the off response in RA and PC units to a ramp stimulation, the persistence of discharges of the SA units during steady pressure on the receptive field and the classical tuning curve seen in the PC units. A few PC units in the hairy skin were also studied for comparison. 3. Lamellated corpuscles were found histologically in the skin of the receptive field of RA units and identified as Krause's corpuscle of cylindrical type by their superficial location in the cutaneous tissue and their structure revealed by electron microscopy. 4. Physiological characteristics of RA units to various forms of mechanical stimulation were studied and compared with those of the other two kinds of units. SA units had the lowest critical slope among three groups and PC units the highest. 5. The discharge pattern of RA and PC units to a ramp stimulation was found to be time-locked, whereas with SA unites only the first spike appeared at a fixed latency from the start of stimulation. 6. Some RA units showed a tuning curve which was flat from 10 to 200 Hz. Those with narrowly tuned curves had a best turning frequency at around 20 Hz. They were easily differentiated from the SA and PC units. SA units were tuned best at 5 HZ or less, and PC units at around 200 HZ. 7. The relation between the indentation velocity and amplitude of the ramp and the spike discharges was analysed in eleven RA units. In most cases the relation between identation velocity and maximum instataneous frequency was found to be best fit with a power function although other kinds of functions (linear, logarithmic, and logarithmic hyperbolic tangent) could also fit
Rayan, A M; Farghali, K A
Under natural drought, some physiological parameters were measured in some wild species inhabiting the western desert of Egypt. Seasonal changes of nitrogen metabolites and Na/K ratio were detected in the investigated species. Effect of seasons, species, and their interaction played an important role on total free amino acids, soluble proteins and Na/K ratio at two oases (Dakhla and Kharga). Species diversity showed more effective variable in regulating such metabolites at Kharga oasis. Plants responded to their environment in two ways, either by increasing their water binding molecules or by preventing the formation of amino acids into proteins. Some of the halophytic and xerophytic species may adjust osmotically to stress by the contribution of nitrogen metabolites. On the other hand, Zygophyllum coccineum, the succulent plant, may adapt to environmental conditions through the accumulation of free amino acids. Correlation analysis between Na+/K+ ratio with free amino acids, soluble proteins and water content in Tamarix aphylla, Salsola imbricata, Balanites aegyptiaca, Trichodesma africanum, and Z. coccineum (Kharga) indicated changes in ionic fraction or accumulating soluble organic compounds which were osmotically active and contribute to osmotic adjustment. Correlations were found between chlorophyll content, ionic and nitrogen metabolites. In Acacia nilotica, Suaeda monoica and Z. coccineum at Dakhla oasis, changes in soluble proteins or ionic ratio could be caused by chlorophyll response to stress, while S. imbricata and T. aphylla may control cellular protein contents. On the other hand, the sharing of both free amino acids and ionic fraction may play an important role of osmoregulation in S. imbricata, Citrullus colocynthis and Z. coccineum at Kharga oasis. (author)
This bachelor thesis titled Nutrition of the transition cow deals with nutrition of dairy cows in peripartum period with regard to prevention of development of metabolic diseases. Anatomy of digestive system and physiology of digestive processes are briefly described. Characteristic of nutrients and of the most common feeds used for nutrition of dairy cattle serves as introduction to formulation of dairy rations. Metabolic diseases caused by inadequate nutrition in transition period are the b...
Koubková, M.; Knížková, I.; Kunc, P.; Hartlová, H.; Flusser, Jan
Roč. 47, č. 8 (2002), s. 309-318 ISSN 1212-1819 R&D Projects: GA ČR GA523/99/1489 Institutional research plan: CEZ:AV0Z1075907 Keywords : dairy cows * heat stress * blood samples Subject RIV: GH - Livestock Nutrition Impact factor: 0.187, year: 2002
Vorup Petersen, Jacob
function, and 3) the feasibility, motivation and injury rate of regular small-sided ball games in untrained elderly. Purpose. The overall aims of the present thesis were to examine physiological adaptations important for health after a period of small-sided ball game training and protein ingestion...... adults, 16 weeks of small-sided soccer was shown to improve physical function and exercise capacity, whereas muscle mass was unaffected by the training despite a high intake of daily protein. However, a number of questions still need to be answered regarding small-sided ball game training in untrained...... elderly: 1) The effect of other small-sided ball games, e.g. floorball training and cone ball, on physiological adaptations important for health as well as the effect of combined protein intake, 2) the effect of regular small-sided ball games in older adults with a more advanced age and low physical...
Vorup Petersen, Jacob
Introduction. Aging is associated with a physiological decline that contributes to loss of physical function and increased risk of adverse cardiovascular events and development of type 2 diabetes. The vast majority of research has focused on traditional exercise activities such as brisk walking......, cycling or resistance training in the prevention of this physiological decline with age. However, untrained elderly may be reluctant to participate in multiple training sessions a week, and may also be unwilling to take part in intense exercise due to motivational reasons. Thus, efficient and motivating...... elderly: 1) The effect of other small-sided ball games, e.g. floorball training and cone ball, on physiological adaptations important for health as well as the effect of combined protein intake, 2) the effect of regular small-sided ball games in older adults with a more advanced age and low physical...
Sep 20, 2012 ... terms of longevity and culling reasons were investigated. For the group of PHF cows, ... breeding work, there has been a considerable increase in productivity .... day of cow's life, it was found that as far as the pro- ductive life ...
Broom, Donald M
welfare can be very good when it is occurring. Other adaptation is difficult and may involve lower or higher level emergency physiological responses or abnormal behaviour, often with bad feelings such as pain or fear. In that case, welfare is poor or very poor even if complete adaptation eventually occurs and there is no long-term threat to the life of the individual. In some circumstances, adaptation may be unsuccessful, the individual is not able to cope, stress occurs and welfare is ultimately very poor.
Lopes van Balen, V A; van Gansewinkel, T A G; de Haas, S; van Kuijk, S M J; van Drongelen, J; Ghossein-Doha, C; Spaanderman, M E A
To establish reference values for flow-mediated dilatation (FMD) and brachial artery diameter (BAD) in pregnancy and to provide insight into the physiological and pathological course of endothelial adaptation throughout human singleton pregnancy. A meta-analysis was performed following a systematic review of current literature on FMD, as a derivative for endothelial function, and BAD, throughout uncomplicated and complicated pregnancy. PubMed (NCBI) and EMBASE (Ovid) electronic databases were used for the literature search, which was performed from inception to 9 June 2016. To allow judgment of changes in comparison with the non-pregnant state, studies were required to report both non-pregnant mean reference of FMD (matched control group, prepregnancy or postpartum measurement) and mean FMD at a predetermined and reported gestational age. Pooled mean differences between the reference and pregnant FMD values were calculated for predefined intervals of gestational age. Fourteen studies that enrolled 1231 participants met the inclusion criteria. Publication dates ranged from 1999 to 2014. In uncomplicated pregnancy, FMD was increased in the second and third trimesters. Between 15 and 21 weeks of gestation, absolute FMD increased the most, by a mean (95% CI) of 1.89% (0.25-3.53%). This was a relative increase of 22.5% (3.0-42.0%) compared with the non-pregnant reference. BAD increased progressively, in a steady manner, by the second trimester but not significantly in the first half of the second trimester. We could not discern differences in FMD and BAD between complicated and uncomplicated pregnancies at 29-35 weeks' gestation, reported in the three studies that met our inclusion criteria. Despite the increase in FMD and BAD throughout gestation, both reference curves were characterized by wide 95% CIs. During healthy pregnancy, endothelium-dependent vasodilatation and BAD increase. Women with a complicated pregnancy had FMD values within the lower range when
De Koster, Jenne D; Opsomer, Geert
Glucose is the molecule that drives milk production, and insulin plays a pivotal role in the glucose metabolism of dairy cows. The effect of insulin on the glucose metabolism is regulated by the secretion of insulin by the pancreas and the insulin sensitivity of the skeletal muscles, the adipose tissue, and the liver. Insulin resistance may develop as part of physiologic (pregnancy and lactation) and pathologic processes, which may manifest as decreased insulin sensitivity or decreased insulin responsiveness. A good knowledge of the normal physiology of insulin is needed to measure the in vivo insulin resistance of dairy cows. Copyright © 2013 Elsevier Inc. All rights reserved.
... Safe Videos for Educators Search English Español Mad Cow Disease KidsHealth / For Teens / Mad Cow Disease What's ... are people to get it? What Is Mad Cow Disease? Mad cow disease is an incurable, fatal ...
Full Text Available For large-scale network simulations, it is often desirable to have computationally tractable, yet in a defined sense still physiologically valid neuron models. In particular, these models should be able to reproduce physiological measurements, ideally in a predictive sense, and under different input regimes in which neurons may operate in vivo. Here we present an approach to parameter estimation for a simple spiking neuron model mainly based on standard f-I curves obtained from in vitro recordings. Such recordings are routinely obtained in standard protocols and assess a neuron's response under a wide range of mean input currents. Our fitting procedure makes use of closed-form expressions for the firing rate derived from an approximation to the adaptive exponential integrate-and-fire (AdEx model. The resulting fitting process is simple and about two orders of magnitude faster compared to methods based on numerical integration of the differential equations. We probe this method on different cell types recorded from rodent prefrontal cortex. After fitting to the f-I current-clamp data, the model cells are tested on completely different sets of recordings obtained by fluctuating ('in-vivo-like' input currents. For a wide range of different input regimes, cell types, and cortical layers, the model could predict spike times on these test traces quite accurately within the bounds of physiological reliability, although no information from these distinct test sets was used for model fitting. Further analyses delineated some of the empirical factors constraining model fitting and the model's generalization performance. An even simpler adaptive LIF neuron was also examined in this context. Hence, we have developed a 'high-throughput' model fitting procedure which is simple and fast, with good prediction performance, and which relies only on firing rate information and standard physiological data widely and easily available.
National Aeronautics and Space Administration — In this study transcriptome profiling was used to gain insight into the spaceflight adaptation role of Altered response to gravity-1 (Arg1) a gene known to affect...
Haas, S.; Ghossein-Doha, C.; Kuijk, S.M. van; Drongelen, J. van; Spaanderman, M.E.A.
OBJECTIVE: To describe the physiological pattern of gestational plasma volume adjustments in normal singleton pregnancy and compare this with the pattern in pregnancies complicated by pregnancy-induced hypertension, pre-eclampsia or fetal growth restriction. METHODS: We performed a meta-analysis of
Antoni, Michael H; Lechner, Suzanne; Diaz, Alain; Vargas, Sara; Holley, Heather; Phillips, Kristin; McGregor, Bonnie; Carver, Charles S; Blomberg, Bonnie
A diagnosis of breast cancer and treatment are psychologically stressful events, particularly over the first year after diagnosis. Women undergo many demanding and anxiety-arousing treatments such as surgery, radiation and chemotherapy. Psychosocial interventions that promote psychosocial adaptation to these challenges may modulate physiological processes (neuroendocrine and immune) that are relevant for health outcomes in breast cancer patients. Women with Stages 1-3 breast cancer recruited 4-8 weeks after surgery were randomized to either a 10-week group-based cognitive behavioral stress management (CBSM) intervention or a 1-day psychoeducational control group and completed questionnaires and late afternoon blood samples at study entry and 6 and 12 months after assignment to experimental condition. Of 128 women initially providing psychosocial questionnaire and blood samples at study entry, 97 provided complete data for anxiety measures and cortisol analysis at all time points, and immune assays were run on a subset of 85 of these women. Those assigned to a 10-week group-based CBSM intervention evidenced better psychosocial adaptation (lower reported cancer-specific anxiety and interviewer-rated general anxiety symptoms) and physiological adaptation (lower cortisol, greater Th1 cytokine [interleukin-2 and interferon-gamma] production and IL-2:IL-4 ratio) after their adjuvant treatment compared to those in the control group. Effects on psychosocial adaptation indicators and cortisol appeared to hold across the entire 12-month observation period. Th1 cytokine regulation changes held only over the initial 6-month period. This intervention may have facilitated a "recovery or maintenance" of Th1 cytokine regulation during or after the adjuvant therapy period. Behavioral interventions that address dysregulated neuroendocrine function could play a clinically significant role in optimizing host immunologic resistance during a vulnerable period.
Kinoshita, Asako; Kenéz, Ákos; Locher, Lena; Meyer, Ulrich; Dänicke, Sven; Rehage, Jürgen; Huber, Korinna
The glucose homeostasis in dairy cattle is very well controlled, in line with the metabolic adaptation during the periparturient period. Former studies showed that nicotinic acid (NA) lowered plasma non-esterified fatty acids (NEFA) concentrations and increased insulin sensitivity in dairy cows. Thus, the purpose of this study was to investigate whether the expression of proteins involved in hepatic and adipose insulin signaling and protein expression of hepatic glucose transporter 2 (GLUT2) were affected by dietary NA and dietary concentrate intake in periparturient dairy cows. Twenty pluriparous German Holstein cows were fed with the same diet from about 21 days before the expected calving date (d-21) to calving. After calving, cows were randomly assigned in 4 groups and fed with diets different in concentrate proportion ("HC" with 60:40% or "LC" with 30:70% concentrate-to-roughage ratio) and supplemented with NA (24 g/day) (NA) or without (CON) until d21. Biopsy samples were taken from the liver, subcutaneous (SCAT) and retroperitoneal (RPAT) adipose tissues at d-21 and d21. Protein expression of insulin signaling molecules (insulin receptor (INSR), phosphatidylinositol-3-kinase (PI3K), protein kinase Cζ (PKCζ)) and hepatic GLUT2 was measured by Western Blotting. The ratio of protein expression at d21/at d-21 was calculated and statistically evaluated for the effects of time and diet. Cows in HC had significantly higher dietary energy intake than cows in LC. In RPAT a decrease in PI3K and PKCζ expression was found in all groups, irrespectively of diet. In the liver, the GLUT2 expression was significantly lower in cows in NA compared with cows in CON. In conclusion, insulin signaling might be decreased in RPAT over time without any effect of diet. NA was able to modulate hepatic GLUT2 expression, but its physiological role is unclear.
Full Text Available The glucose homeostasis in dairy cattle is very well controlled, in line with the metabolic adaptation during the periparturient period. Former studies showed that nicotinic acid (NA lowered plasma non-esterified fatty acids (NEFA concentrations and increased insulin sensitivity in dairy cows. Thus, the purpose of this study was to investigate whether the expression of proteins involved in hepatic and adipose insulin signaling and protein expression of hepatic glucose transporter 2 (GLUT2 were affected by dietary NA and dietary concentrate intake in periparturient dairy cows. Twenty pluriparous German Holstein cows were fed with the same diet from about 21 days before the expected calving date (d-21 to calving. After calving, cows were randomly assigned in 4 groups and fed with diets different in concentrate proportion ("HC" with 60:40% or "LC" with 30:70% concentrate-to-roughage ratio and supplemented with NA (24 g/day (NA or without (CON until d21. Biopsy samples were taken from the liver, subcutaneous (SCAT and retroperitoneal (RPAT adipose tissues at d-21 and d21. Protein expression of insulin signaling molecules (insulin receptor (INSR, phosphatidylinositol-3-kinase (PI3K, protein kinase Cζ (PKCζ and hepatic GLUT2 was measured by Western Blotting. The ratio of protein expression at d21/at d-21 was calculated and statistically evaluated for the effects of time and diet. Cows in HC had significantly higher dietary energy intake than cows in LC. In RPAT a decrease in PI3K and PKCζ expression was found in all groups, irrespectively of diet. In the liver, the GLUT2 expression was significantly lower in cows in NA compared with cows in CON. In conclusion, insulin signaling might be decreased in RPAT over time without any effect of diet. NA was able to modulate hepatic GLUT2 expression, but its physiological role is unclear.
PISCHAN S.; LYTVYSCHENKO L.; MARTYNENKO A.; PISCHAN І.
The results of studies of physiological and productive milking cows in the early postnatal period. Proved that nepatolohichna modified organism physiological state accompanied by changes in body temperature, milk production and quality of dairy animals.
Romero-Lopez, Julia; Lopez-Rodas, Victoria [Genetica, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, E-28040 Madrid (Spain); Costas, Eduardo, E-mail: firstname.lastname@example.org [Genetica, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, E-28040 Madrid (Spain)
Highlights: Black-Right-Pointing-Pointer Microalgae are able to physiological acclimatization low doses of petroleum and diesel oil. Black-Right-Pointing-Pointer When petroleum or oil concentration exceeds these limits, survival depend of rare mutations. Black-Right-Pointing-Pointer Petroleum-resistant and diesel oil mutants occur spontaneously prior to oil exposure. Black-Right-Pointing-Pointer After 300 generations of artificial selection resistant strains were obtained. Black-Right-Pointing-Pointer Cyanobacteria has more difficulties to achieve petroleum resistance than Chlorophyta. - Abstract: There is increasing scientific interest in how phytoplankton reacts to petroleum contamination, since crude oil and its derivatives are generating extensive contamination of aquatic environments. However, toxic effects of short-term petroleum exposure are more widely known than the adaptation of phytoplankton to long-term petroleum exposure. An analysis of short-term and long-term effects of petroleum exposure was done using experimental populations of freshwater (Scenedesmus intermedius and Microcystis aeruginosa) and marine (Dunaliella tertiolecta) microalgae isolated from pristine sites without crude oil product contamination. These strains were exposed to increased levels of petroleum and diesel oil. Short-term exposure to petroleum or diesel oil revealed a rapid inhibition of photosynthetic performance and cell proliferation in freshwater and marine phytoplankton species. A broad degree of inter-specific variation in lethal contamination level was observed. When different strains were exposed to petroleum or diesel oil over the long-term, the cultures showed massive destruction of the sensitive cells. Nonetheless, after further incubation, some cultures were able to grow again due to cells that were resistant to the toxins. By means of a fluctuation analysis, discrimination between cells that had become resistant due to physiological acclimatization and resistant
Romero-Lopez, Julia; Lopez-Rodas, Victoria; Costas, Eduardo
Highlights: ► Microalgae are able to physiological acclimatization low doses of petroleum and diesel oil. ► When petroleum or oil concentration exceeds these limits, survival depend of rare mutations. ► Petroleum-resistant and diesel oil mutants occur spontaneously prior to oil exposure. ► After 300 generations of artificial selection resistant strains were obtained. ► Cyanobacteria has more difficulties to achieve petroleum resistance than Chlorophyta. - Abstract: There is increasing scientific interest in how phytoplankton reacts to petroleum contamination, since crude oil and its derivatives are generating extensive contamination of aquatic environments. However, toxic effects of short-term petroleum exposure are more widely known than the adaptation of phytoplankton to long-term petroleum exposure. An analysis of short-term and long-term effects of petroleum exposure was done using experimental populations of freshwater (Scenedesmus intermedius and Microcystis aeruginosa) and marine (Dunaliella tertiolecta) microalgae isolated from pristine sites without crude oil product contamination. These strains were exposed to increased levels of petroleum and diesel oil. Short-term exposure to petroleum or diesel oil revealed a rapid inhibition of photosynthetic performance and cell proliferation in freshwater and marine phytoplankton species. A broad degree of inter-specific variation in lethal contamination level was observed. When different strains were exposed to petroleum or diesel oil over the long-term, the cultures showed massive destruction of the sensitive cells. Nonetheless, after further incubation, some cultures were able to grow again due to cells that were resistant to the toxins. By means of a fluctuation analysis, discrimination between cells that had become resistant due to physiological acclimatization and resistant cells arising from rare spontaneous mutations was accomplished. In addition, an analysis was done as to the maximum capacity of
Cohen, Alan A
The longstanding debate about whether aging may have evolved for some adaptive reason is generally considered to pit evolutionary theory against empirical observations consistent with aging as a programmed aspect of organismal biology, in particular conserved aging genes. Here I argue that the empirical evidence on aging mechanisms does not support a view of aging as a programmed phenomenon, but rather supports a view of aging as the dysregulation of complex networks that maintain organismal homeostasis. The appearance of programming is due largely to the inadvertent activation of existing pathways during the process of dysregulation. It is argued that aging differs markedly from known programmed biological phenomena such as apoptosis in that it is (a) very heterogeneous in how it proceeds, and (b) much slower than it would need to be. Furthermore, the taxonomic distribution of aging across species does not support any proposed adaptive theories of aging, which would predict that aging rate would vary on a finer taxonomic scale depending on factors such as population density. Thus, while there are problems with the longstanding non-adaptive paradigm, current evidence does not support the notion that aging is programmed or that it may have evolved for adaptive reasons.
Laboratory mice housed under standard vivarium conditions with an ambient temperature (Ta) of -22°C are likely to be cold stressed because this Ta is below their thermoneutral zone (TNZ). Mice raised at Tas within the TNZ adapt to the warmer temperatures, developing smaller int...
Joseph S Uzarski
Full Text Available Endothelial cell (EC function is mediated by variable hemodynamic shear stress patterns at the vascular wall, where complex shear stress profiles directly correlate with blood flow conditions that vary temporally based on metabolic demand. The interactions of these more complex and variable shear fields with EC have not been represented in hemodynamic flow models. We hypothesized that EC exposed to pulsatile shear stress that changes in magnitude and duration, modeled directly from real-time physiological variations in heart rate, would elicit phenotypic changes as relevant to their critical roles in thrombosis, hemostasis, and inflammation. Here we designed a physiological flow (PF model based on short-term temporal changes in blood flow observed in vivo and compared it to static culture and steady flow (SF at a fixed pulse frequency of 1.3 Hz. Results show significant changes in gene regulation as a function of temporally variable flow, indicating a reduced wound phenotype more representative of quiescence. EC cultured under PF exhibited significantly higher endothelial nitric oxide synthase (eNOS activity (PF: 176.0±11.9 nmol/10(5 EC; SF: 115.0±12.5 nmol/10(5 EC, p = 0.002 and lower TNF-a-induced HL-60 leukocyte adhesion (PF: 37±6 HL-60 cells/mm(2; SF: 111±18 HL-60/mm(2, p = 0.003 than cells cultured under SF which is consistent with a more quiescent anti-inflammatory and anti-thrombotic phenotype. In vitro models have become increasingly adept at mimicking natural physiology and in doing so have clarified the importance of both chemical and physical cues that drive cell function. These data illustrate that the variability in metabolic demand and subsequent changes in perfusion resulting in constantly variable shear stress plays a key role in EC function that has not previously been described.
Khalilieh, Anton; McCue, Marshall D; Pinshow, Berry
Many wild birds fast during reproduction, molting, migration, or because of limited food availability. Species that are adapted to fasting sequentially oxidize endogenous fuels in three discrete phases. We hypothesized that species not adapted to long fasts have truncated, but otherwise similar, phases of fasting, sequential changes in fuel oxidization, and similar changes in blood metabolites to fasting-adapted species. We tested salient predictions in house sparrows (Passer domesticus biblicus), a subspecies that is unable to tolerate more than ~32 h of fasting. Our main hypothesis was that fasting sparrows sequentially oxidize substrates in the order carbohydrates, lipids, and protein. We dosed 24 house sparrows with [(13)C]glucose, palmitic acid, or glycine and measured (13)CO(2) in their breath while they fasted for 24 h. To ascertain whether blood metabolite levels reflect fasting-induced changes in metabolic fuels, we also measured glucose, triacylglycerides, and β-hydroxybutyrate in the birds' blood. The results of both breath (13)CO(2) and plasma metabolite analyses did not support our hypothesis; i.e., that sparrows have the same metabolic responses characteristic of fasting-adapted species, but on a shorter time scale. Contrary to our main prediction, we found that recently assimilated (13)C-tracers were oxidized continuously in different patterns with no definite peaks corresponding to the three phases of fasting and also that changes in plasma metabolite levels accurately tracked the changes found by breath analysis. Notably, the rate of recently assimilated [(13)C]glycine oxidization was significantly higher (P fast for longer than 32 h is likely related to their inability to accrue large lipid stores, separately oxidize different fuels, and/or spare protein during fasting.
Tyler, Christopher J; Reeve, Tom; Hodges, Gary J; Cheung, Stephen S
Exercise performance and capacity are impaired in hot, compared to temperate, conditions. Heat adaptation (HA) is one intervention commonly adopted to reduce this impairment because it may induce beneficial exercise performance and physiological and perceptual adaptations. A number of investigations have been conducted on HA but, due to large methodological differences, the effectiveness of different HA regimens remain unclear. (1) To quantify the effect of different HA regimens on exercise performance and the physiological and perceptual responses to subsequent heat exposure. (2) To offer practical HA recommendations and suggestions for future HA research based upon a systematic and quantitative synthesis of the literature. PubMed was searched for original research articles published up to, and including, 16 February 2016 using appropriate first- and second-order search terms. English-language, peer-reviewed, full-text original articles using human participants were reviewed using the four-stage process identified in the PRISMA statement. Data for the following variables were obtained from the manuscripts by at least two of the authors: participant sex, maximal oxygen consumption and age; HA duration, frequency, modality, temperature and humidity; exercise performance and capacity; core and skin temperature; heart rate, stroke volume, cardiac output, skin blood flow, sweat onset temperature, body mass loss, sweat rate, perception of thirst, volitional fluid consumption, plasma volume changes; sweat concentrations of sodium, chloride and potassium; aldosterone, arginine vasopressin, heat shock proteins (Hsp), ratings of perceived exertion (RPE) and thermal sensation. Data were divided into three groups based upon the frequency of the HA regimen. Performance and capacity data were also divided into groups based upon the type of HA used. Hedges' g effect sizes and 95 % confidence intervals were calculated. Correlations were run where appropriate. Ninety-six articles
Iaia, F. M.; Bangsbo, Jens
the oxidative capacity and improve intense short-duration/repeated high-intensity exercise performance lasting 30 s to 4 min, as it occurs in a number of sports. When combined with a basic volume of training including some aerobic high-intensity sessions, speed endurance training is also useful in enhancing......The present article reviews the physiological and performance effects of speed endurance training consisting of exercise bouts at near maximal intensities in already trained subjects. Despite a reduction in training volume, speed endurance training of endurance-trained athletes can maintain...... performance during longer events, e.g. 40 K cycling and 10 K running. Athletes in team sports involving intense exercise actions and endurance aspects can also benefit from performing speed endurance training. These improvements don't appear to depend on changes in maximum oxygen uptake (VO2max), muscle...
Stefan De Smet
Full Text Available In the setting of “living high,” it is unclear whether high-intensity interval training (HIIT should be performed “low” or “high” to stimulate muscular and performance adaptations. Therefore, 10 physically active males participated in a 5-week “live high-train low or high” program (TR, whilst eight subjects were not engaged in any altitude or training intervention (CON. Five days per week (~15.5 h per day, TR was exposed to normobaric hypoxia simulating progressively increasing altitude of ~2,000–3,250 m. Three times per week, TR performed HIIT, administered as unilateral knee-extension training, with one leg in normobaric hypoxia (~4,300 m; TRHYP and with the other leg in normoxia (TRNOR. “Living high” elicited a consistent elevation in serum erythropoietin concentrations which adequately predicted the increase in hemoglobin mass (r = 0.78, P < 0.05; TR: +2.6%, P < 0.05; CON: −0.7%, P > 0.05. Muscle oxygenation during training was lower in TRHYP vs. TRNOR (P < 0.05. Muscle homogenate buffering capacity and pH-regulating protein abundance were similar between pretest and posttest. Oscillations in muscle blood volume during repeated sprints, as estimated by oscillations in NIRS-derived tHb, increased from pretest to posttest in TRHYP (~80%, P < 0.01 but not in TRNOR (~50%, P = 0.08. Muscle capillarity (~15% as well as repeated-sprint ability (~8% and 3-min maximal performance (~10–15% increased similarly in both legs (P < 0.05. Maximal isometric strength increased in TRHYP (~8%, P < 0.05 but not in TRNOR (~4%, P > 0.05. In conclusion, muscular and performance adaptations were largely similar following normoxic vs. hypoxic HIIT. However, hypoxic HIIT stimulated adaptations in isometric strength and muscle perfusion during intermittent sprinting.
Manor, Brad; Costa, Madalena D; Hu, Kun; Newton, Elizabeth; Starobinets, Olga; Kang, Hyun Gu; Peng, C K; Novak, Vera; Lipsitz, Lewis A
The degree of multiscale complexity in human behavioral regulation, such as that required for postural control, appears to decrease with advanced aging or disease. To help delineate causes and functional consequences of complexity loss, we examined the effects of visual and somatosensory impairment on the complexity of postural sway during quiet standing and its relationship to postural adaptation to cognitive dual tasking. Participants of the MOBILIZE Boston Study were classified into mutually exclusive groups: controls [intact vision and foot somatosensation, n = 299, 76 ± 5 (SD) yr old], visual impairment only (Postural sway (i.e., center-of-pressure) dynamics were assessed during quiet standing and cognitive dual tasking, and a complexity index was quantified using multiscale entropy analysis. Postural sway speed and area, which did not correlate with complexity, were also computed. During quiet standing, the complexity index (mean ± SD) was highest in controls (9.5 ± 1.2) and successively lower in the visual (9.1 ± 1.1), somatosensory (8.6 ± 1.6), and combined (7.8 ± 1.3) impairment groups (P = 0.001). Dual tasking resulted in increased sway speed and area but reduced complexity (P postural sway speed from quiet standing to dual-tasking conditions. Sensory impairments contributed to decreased postural sway complexity, which reflected reduced adaptive capacity of the postural control system. Relatively low baseline complexity may, therefore, indicate control systems that are more vulnerable to cognitive and other stressors.
Mariggiò, Maria A; Falone, Stefano; Morabito, Caterina; Guarnieri, Simone; Mirabilio, Alessandro; Pilla, Raffaele; Bucciarelli, Tonino; Verratti, Vittore; Amicarelli, Fernanda
Depending on the absolute altitude and the duration of exposure, a high altitude environment induces various cellular effects that are strictly related to changes in oxidative balance. In this study, we used in vitro isolated peripheral blood lymphocytes as biosensors to test the effect of hypobaric hypoxia on seven climbers by measuring the functional activity of these cells. Our data revealed that a 21-day exposure to high altitude (5000 m) (1) increased intracellular Ca(2+) concentration, (2) caused a significant decrease in mitochondrial membrane potential, and (3) despite possible transient increases in intracellular levels of reactive oxygen species, did not significantly change the antioxidant and/or oxidative damage-related status in lymphocytes and serum, assessed by measuring Trolox-equivalent antioxidant capacity, glutathione peroxidase activity, vitamin levels, and oxidatively modified proteins and lipids. Overall, these results suggest that high altitude might cause an impairment in adaptive antioxidant responses. This, in turn, could increase the risk of oxidative-stress-induced cellular damage. In addition, this study corroborates the use of peripheral blood lymphocytes as an easily handled model for monitoring adaptive response to environmental challenge.
Tienken, Reka; Kersten, Susanne; Frahm, Jana; Hüther, Liane; Meyer, Ulrich; Huber, Korinna; Rehage, Jürgen; Dänicke, Sven
Simple Summary Several biological changes occur during the transition from late pregnancy to early lactation which is associated with a high susceptibility of health disorders. Nicotinic acid, as feed additive, is suggested to balance catabolic metabolism of periparturient dairy cows by attenuating lipolysis and impact production performance. This study provides information of the biological changes occurring around parturition with special emphasis on differences between primiparous and multiparous cows. Present results showed that energy-dense feeding prepartum did not result in metabolic imbalances postpartum in dairy cows which were similar in body condition score. Nicotinic acid supplementation did not reveal any effect. Abstract The periparturient period is critical according to health, productivity and profitability. As this period is fundamental for the success of the lactation period, the interest in improving periparturient health by dietary supplements increased in recent years. The present study investigated the effects of feeding nicotinic acid (NA) combined with varying dietary energy densities on immunological, hematological and biochemical parameters of periparturient cows differing in parity. Thirty-six multiparous and 20 primiparous dairy cows were enrolled in the study 42 days before expected parturition date until 100 days postpartum with the half of the cows being supplemented with 24 g of NA/d. After parturition a diet with 30% concentrate was fed to all cows which was followed by different concentrate escalation strategies. Dietary NA supplementation was ceased on day 24 postpartum. Dietary NA increased (P = 0.010) serum nicotinamide concentrations (mean of 3.35 ± 1.65 µg/mL), whereas NA could not be detected. Present data emphasize that periparturient cows are faced with major physiological challenges and that both parity-groups have different prerequisites to adapt to those changes irrespective of NA supplementation. The overfeeding of
Sang, Hongqiang; Yang, Chenghao; Liu, Fen; Yun, Jintian; Jin, Guoguang; Chen, Fa
Hand physiological tremor of surgeons can cause vibration at the surgical instrument tip, which may make it difficult for the surgeon to perform fine manipulations of tissue, needles, and sutures. A zero phase adaptive fuzzy Kalman filter (ZPAFKF) is proposed to suppress hand tremor and vibration of a robotic surgical system. The involuntary motion can be reduced by adding a compensating signal that has the same magnitude and frequency but opposite phase with the tremor signal. Simulations and experiments using different filters were performed. Results show that the proposed filter can avoid the loss of useful motion information and time delay, and better suppress minor and varying tremor. The ZPAFKF can provide less error, preferred accuracy, better tremor estimation, and more desirable compensation performance, to suppress hand tremor and decrease vibration at the surgical instrument tip. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.
Weber, Roy E.
In transporting O2 from the respiratory surfaces to the respiring tissues of animals, hemoglobin (Hb) directly links aerobic metabolism with O2 availability and is a paradigm for studying mechanisms of molecular adaptations. Hb-O2 binding is cooperative (described by sigmoid O2 binding curves......) and decreased by allosteric effectors (protons, CO2, lactate, organic phosphates and chloride ions) that modulate O2 binding in response to changes in environmental and metabolic dictates. Hb-O2 affinity moreover decreases with rising temperature. This increases O2 unloading in warm tissues that consume more O2......, but may be maladaptive – and thus is reduced - in regional heterothermic animals where it may hamper O2 unloading (in cold extremities of Artic mammals) or cause excessive O2 release (in warm organs of fast-swimming fish). Illustrated with case studies (estivating lungfish, high altitude frogs, birds...
de Haas, S; Ghossein-Doha, C; van Kuijk, S M J; van Drongelen, J; Spaanderman, M E A
To describe the physiological pattern of gestational plasma volume adjustments in normal singleton pregnancy and compare this with the pattern in pregnancies complicated by pregnancy-induced hypertension, pre-eclampsia or fetal growth restriction. We performed a meta-analysis of the current literature on plasma volume adjustments during physiological and complicated pregnancies. Literature was retrieved from PubMed (NCBI) and EMBASE (Ovid) databases. Included studies reported both reference plasma volume measurements (non-pregnant, prepregnancy or postpartum) and measurements obtained during predetermined gestational ages. Mean differences bet ween the reference and pregnancy plasma volume measurements were calculated for predefined intervals of gestational age using a random-effects model described by DerSimonian and Laird. Thirty studies were included in the meta-analysis with publication dates ranging from 1934 to 2007. Plasma volume increased in the first weeks of pregnancy, with the steepest increase occurring during the second trimester. Plasma volume continued to increase in the third trimester with a pooled maximum increase of 1.13 L (95% CI, 1.07-1.19 L), an increase of 45.6% (95% CI, 43.0-48.1%) in physiological pregnancies compared with the reference value. The plasma volume expansion in gestational hypertensive and growth-restricted pregnancies was 0.80 L (95% CI, 0.59-1.02 L), an increase of 32.3% (95% CI, 23.6-41.1%) in the third trimester, a smaller increase than in physiological pregnancies (P embarazo: una revisi\\xF3n sistemática y metaanálisis RESUMEN OBJETIVO: Describir el patrón fisiológico de los cambios en el volumen del plasma gestacional en embarazos normales con feto único y compararlo con el patrón en los embarazos complicados por hipertensión gestacional, preeclampsia o restricción del crecimiento fetal. MÉTODOS: Se realizó un metaanálisis de la literatura actual sobre los cambios en el volumen de plasma durante
Mustonen, Anne-Mari; Bowman, Jeff; Sadowski, Carrie; Nituch, Larissa A; Bruce, Laura; Halonen, Toivo; Puukka, Katri; Rouvinen-Watt, Kirsti; Aho, Jari; Nieminen, Petteri
Wintertime physiology of captive striped skunks (Mephitis mephitis) in response to cold ambient temperature (Ta) and fasting was investigated with body temperature (Tb) and activity recordings and analyses of hematology, plasma biochemistry and tissue fatty acids (FA). After 105 days of food deprivation, the skunks were in phase II of fasting indicated by the elevated plasma nonesterified FA and glycerol but no accumulation of nitrogen end products. Shorter-chain saturated and monounsaturated FA together with C18-20 n-3 polyunsaturated FA were preferentially mobilized. Individual amino acids responded to fasting in a complex manner, while essential and nonessential amino acid sums remained stable. Increases in hemoglobin and hematocrit suggested dehydration. The activity levels were lower in mid-January-early March, and the activity bouts were mostly displayed between 17:00-23:00 h. Daily torpor was observed in two females with 29 and 46 bouts. The deepest torpor (Tb<31 °C) occurred between dawn and early afternoon and lasted for 3.3 ± 0.18 h. The average minimum Tb was 29.2 ± 0.15 °C and the lowest recorded Tb was 25.8 °C. There was significant relation between the average 24-h Tb and Ta. Increases in wintertime Ta, as predicted by climate change scenarios, could influence torpor patterns in the species. © 2013.
Polle, Andrea; Chen, Shaoliang
Saline and sodic soils that cannot be used for agriculture occur worldwide. Cultivating stress-tolerant trees to obtain biomass from salinized areas has been suggested. Various tree species of economic importance for fruit, fibre and timber production exhibit high salinity tolerance. Little is known about the mechanisms enabling tree crops to cope with high salinity for extended periods. Here, the molecular, physiological and anatomical adjustments underlying salt tolerance in glycophytic and halophytic model tree species, such as Populus euphratica in terrestrial habitats, and mangrove species along coastlines are reviewed. Key mechanisms that have been identified as mediating salt tolerance are discussed at scales from the genetic to the morphological level, including leaf succulence and structural adjustments of wood anatomy. The genetic and transcriptomic bases for physiological salt acclimation are salt sensing and signalling networks that activate target genes; the target genes keep reactive oxygen species under control, maintain the ion balance and restore water status. Evolutionary adaptation includes gene duplication in these pathways. Strategies for and limitations to tree improvement, particularly transgenic approaches for increasing salt tolerance by transforming trees with single and multiple candidate genes, are discussed. © 2014 John Wiley & Sons Ltd.
Tao, S; Dahl, G E
In dairy cattle, late gestation is a critical period for fetal growth and physiological transition into the next lactation. Environmental factors, such as temperature and light, exert dramatic effects on the production, health, and well-being of animals during this period and after parturition. The aim of this review was to introduce effects of heat stress during late gestation on dairy cattle, and discuss the biological mechanisms that underlie the observed production and health responses in the dam and her fetus. Relative to cooled cows, cows that are heat stressed during late gestation have impaired mammary growth before parturition and decreased milk production in the subsequent lactation. In response to higher milk yield, cows cooled prepartum undergo a series of homeorhetic adaptations in early lactation to meet higher demand for milk synthesis compared with heat-stressed cows, but no direct effect of environmental heat stress on metabolism exists during the dry period. Prepartum cooling improves immune status of transition cows and evidence suggests that altered prolactin signaling in immune cells mediates the effects of heat stress on immune function. Late-gestation heat stress compromises placental development, which results in fetal hypoxia, malnutrition, and eventually fetal growth retardation. Maternal heat stress may also have carryover effects on the postnatal growth of offspring, but direct evidence is still lacking. Emerging evidence suggests that offspring from prepartum heat-stressed cows have compromised passive immunity and impaired cell-mediated immune function compared with those from cooled cows. Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Natsuike, Masafumi; Oikawa, Hiroshi; Matsuno, Kohei; Yamaguchi, Atsushi; Imai, Ichiro
Abundant cyst distributions of the toxic dinoflagellate Alexandrium fundyense (previous A. tamarense north American clade) were recently observed on the north Chukchi Sea shelf and on the eastern Bering Sea shelf, suggesting that A. fundyense is both highly adapted to the local environments in the high latitude areas and might cause toxin contamination of plankton feeders. However, little is known about the physiological characteristics and toxin profiles of A. fundyense in these areas, which are characterized by low water temperatures, weak sunlight, and more or less permanent ice cover during winter. To clarify the physiological characteristics of A. fundyense, the effects of water temperature and light intensity on the vegetative growth and toxin profiles of this species were examined using A. fundyense strains isolated from one sediment sample collected from each area. Using the same sediments samples, seasonal changes of the cyst germination in different water temperatures were investigated. Vegetative cells grew at temperatures as low as 5°C and survived at 1°C under relatively low light intensity. They also grew at moderate water temperatures (10-15°C). Their cysts could germinate at low temperatures (1°C) and have an endogenous dormancy period from late summer to early spring, and warmer water temperatures (5-15°C) increased germination success. These physiological characteristics suggest that A. fundyense in the Chukchi Sea and eastern Bering Sea is adapted to the environments of high latitude areas. In addition, the results suggest that in the study areas A. fundyense has the potential to germinate and grow when water temperatures increase. Cellular toxin amounts of A. fundyense strains from the eastern Bering Sea and Chukchi Sea were ranged from 7.2 to 38.2 fmol cell -1 . These toxin amounts are comparable with A. fundyense strains isolated from other areas where PSP toxin contamination of bivalves occurs. The dominant toxin of the strains isolated
Milk and children; Cow's milk allergy - children; Lactose intolerance - children ... You may have heard that cow's milk should not be given to babies younger than 1 year old. This is because cow's milk doesn't provide enough ...
Paul, Sangeeta; Aggarwal, Chetana; Thakur, Jyoti Kumar; Bandeppa, G. S.; Khan, Md. Aslam; Pearson, Lauren M.; Babnigg, Gyorgy; Giometti, Carol S.; Joachimiak, Andrzej
Bacillus licheniformis strain SSA 61, originally isolated from Sambhar salt lake, was observed to grow even in the presence of 25 % salt stress. Osmoadaptive mechanisms of this halotolerant B. licheniformis strain SSA 61, for long-term survival and growth under salt stress, were determined. Proline was the preferentially accumulated compatible osmolyte. There was also increased accumulation of antioxidants ascorbic acid and glutathione. Among the different antioxidative enzymes assayed, superoxide dismutase played the most crucial role in defense against salt-induced stress in the organism. Adaptation to stress by the organism involved modulation of cellular physiology at various levels. There was enhanced expression of known proteins playing essential roles in stress adaptation, such as chaperones DnaK and GroEL, and general stress protein YfkM and polynucleotide phosphorylase/polyadenylase. Proteins involved in amino acid biosynthetic pathway, ribosome structure, and peptide elongation were also overexpressed. Salt stress-induced modulation of expression of enzymes involved in carbon metabolism was observed. There was up-regulation of a number of enzymes involved in generation of NADH and NADPH, indicating increased cellular demand for both energy and reducing power.
Full Text Available Non-irrigated crops in temperate climates and irrigated crops in arid climates are subjected to continuous cycles of water stress and re-watering. Thus, fast and efficient recovery from water stress may be among the key determinants of plant drought adaptation. The present study was designed to comparatively analyze the roles of drought resistance and drought recovery in drought adaptation and to investigate the physiological basis of genotypic variation in drought adaptation in maize (Zea mays seedlings. As the seedlings behavior in growth associate with yield under drought, it could partly reflect the potential of drought adaptability. Growth and physiological responses to progressive drought stress and recovery were observed in seedlings of ten maize lines. The results showed that drought adaptability is closely related to drought recovery (r = 0.714**, but not to drought resistance (r = 0.332. Drought induced decreases in leaf water content, water potential, osmotic potential, gas exchange parameters, chlorophyll content, Fv/Fm and nitrogen content, and increased H2O2 accumulation and lipid peroxidation. After recovery, most of these physiological parameters rapidly returned to normal levels. The physiological responses varied between lines. Further correlation analysis indicated that the physiological bases of drought resistance and drought recovery are definitely different, and that maintaining higher chlorophyll content (r = 0.874*** and Fv/Fm (r = 0.626* under drought stress contributes to drought recovery. Our results suggest that both drought resistance and recovery are key determinants of plant drought adaptation, and that drought recovery may play a more important role than previously thought. In addition, leaf water potential, chlorophyll content and Fv/Fm could be used as efficient reference indicators in the selection of drought-adaptive genotypes.
Full Text Available Populus x canadensis clone I-214 exhibits a general indicator phenotype in response to excess Zn, and a higher metal uptake in roots than in shoots with a reduced translocation to aerial parts under hydroponic conditions. This physiological adaptation seems mainly regulated by roots, although the molecular mechanisms that underlie these processes are still poorly understood. Here, differential expression analysis using RNA-sequencing technology was used to identify the molecular mechanisms involved in the response to excess Zn in root. In order to maximize specificity of detection of differentially expressed (DE genes, we consider the intersection of genes identified by three distinct statistical approaches (61 up- and 19 down-regulated and validate them by RT-qPCR, yielding an agreement of 93% between the two experimental techniques. Gene Ontology (GO terms related to oxidation-reduction processes, transport and cellular iron ion homeostasis were enriched among DE genes, highlighting the importance of metal homeostasis in adaptation to excess Zn by P. x canadensis clone I-214. We identified the up-regulation of two Populus metal transporters (ZIP2 and NRAMP1 probably involved in metal uptake, and the down-regulation of a NAS4 gene involved in metal translocation. We identified also four Fe-homeostasis transcription factors (two bHLH38 genes, FIT and BTS that were differentially expressed, probably for reducing Zn-induced Fe-deficiency. In particular, we suggest that the down-regulation of FIT transcription factor could be a mechanism to cope with Zn-induced Fe-deficiency in Populus. These results provide insight into the molecular mechanisms involved in adaption to excess Zn in Populus spp., but could also constitute a starting point for the identification and characterization of molecular markers or biotechnological targets for possible improvement of phytoremediation performances of poplar trees.
The decline in dairy cow reproductive performance compromised the productivity and profitability of dairy production worldwide. The phenotypic performance of lactating cows with similar proportions of Holstein genes, similar genetic merit for milk production traits, but either good (Fert+) or poor (Fert-) genetic merit for fertility traits managed in a standardised environment was compared. The objective of this study was to elucidate the physiological mechanisms contributing to suboptimal re...
da Costa, Antônio Nélson Lima; Feitosa, José Valmir; Montezuma, Péricles Afonso; de Souza, Priscila Teixeira; de Araújo, Airton Alencar
This study compared the two breed groups of Girolando (½ Holstein ½ Gyr vs. ¾ Holstein ¼ Gyr) through analysis of the percentages (stressed or non-stressed cows) of rectal temperature (RT), respiratory rate (RR) and pregnancy rate (PR), and means of production and reproduction parameters to determine the group best suited to rearing in semiarid tropical climate. The experiment was conducted at the farm, in the municipality of Umirim, State of Ceará, Brazil. Two hundred and forty cows were used in a 2 × 2 factorial study; 120 of each group were kept under an intensive system during wet and dry seasons. The environmental parameters obtained were relative humidity (RH), air temperature (AT), and the temperature and humidity index (THI). Pregnancy diagnosis (PD) was determined by ultrasonography 30 days after artificial insemination (AI). The milk production of each cow was recorded with automated milkings in the farm. The variables were expressed as mean and standard error, evaluated by ANOVA at 5 % probability using the GLM procedure of SAS. Chi-square test at 5 % probability was applied to data of RT, RR, pregnancy rate (PR), and the number of AIs to obtain pregnancy. The majority of ½ Holstein cows showed mean values of RT and RR within the normal range in both periods and shifts. Most animals of the ¾ Holstein group exhibited the RR means above normal during the afternoon in the rainy and dry periods and RT means above normal during the afternoon in the dry period. After analyses, ½ Holstein crossbred cows are more capable of thermoregulating than ¾ Holstein cows under conditions of thermal stress, and the dry period was more impacting for bovine physiology with significant changes in physiological parameters, even for the first breed group. Knowledge of breed groups adapted to climatic conditions of northeastern Brazil can directly assist cattle farmers in selecting animals best adapted for forming herds.
Coleman, S W; Chase, C C; Riley, D G; Williams, M J
This study was initiated to evaluate performance and patterns of cow traits and blood metabolites of 3 breeds of cows grazing bahiagrass (Paspalum notatum Flügge) pastures in central Florida. Purebred cows (n = 411) of either Angus (Bos taurus), Brahman (Bos indicus), or Romosinuano (Bos taurus) breeding, rotationally grazed (moved twice weekly) bahiagrass pastures year-round, and received bahiagrass hay supplemented with molasses and soyhulls or legume hay supplemented with unfortified molasses from October to June each production year. At monthly intervals, all cows were weighed, measured at the hip (HH), scored for BCS, and blood samples collected by jugular puncture from 10 cows per cow breed/block group for plasma urea N (PUN), glucose and non-esterified fatty acids (NEFA). Data were analyzed on cows that calved with a statistical model that included fixed effects of year, cowage, cow breed, month, block, supplement group (n = 2, but not presented), and whether the cow weaned a calf the previous year. Cow was a repeated observation over mo. Three-way interactions involving monthly patterns for cowage x year, year x lactation status the previous year, cowage × cow breed, year × cow breed, and cow breed × lactation status the previous year were significant (P cow breed × month was important (P cows compared to 3-yr old cows; 2) greater BW and BCS before calving for cows that did not lactate the previous year; 3) PUN levels were above 11 mg/dl except for February, August and September, and was generally greater in tropically adapted breeds; 4) GLU was greatest in Brahman, lowest in Angus, and intermediate in Romosinuano cows; and 5) plasma levels of NEFA escalated at calving and then declined, but Brahman cows maintained greater (P Cows that lactated the previous year had less NEFA than those that did not lactate. Brahman cows were less fertile than Bos taurus breeds, and weaned heavier calves.
Brown, Alastair; Thatje, Sven
Bathymetric biodiversity patterns of marine benthic invertebrates and demersal fishes have been identified in the extant fauna of the deep continental margins. Depth zonation is widespread and evident through a transition between shelf and slope fauna from the shelf break to 1000 m, and a transition between slope and abyssal fauna from 2000 to 3000 m; these transitions are characterised by high species turnover. A unimodal pattern of diversity with depth peaks between 1000 and 3000 m, despite the relatively low area represented by these depths. Zonation is thought to result from the colonisation of the deep sea by shallow-water organisms following multiple mass extinction events throughout the Phanerozoic. The effects of low temperature and high pressure act across hierarchical levels of biological organisation and appear sufficient to limit the distributions of such shallow-water species. Hydrostatic pressures of bathyal depths have consistently been identified experimentally as the maximum tolerated by shallow-water and upper bathyal benthic invertebrates at in situ temperatures, and adaptation appears required for passage to deeper water in both benthic invertebrates and demersal fishes. Together, this suggests that a hyperbaric and thermal physiological bottleneck at bathyal depths contributes to bathymetric zonation. The peak of the unimodal diversity-depth pattern typically occurs at these depths even though the area represented by these depths is relatively low. Although it is recognised that, over long evolutionary time scales, shallow-water diversity patterns are driven by speciation, little consideration has been given to the potential implications for species distribution patterns with depth. Molecular and morphological evidence indicates that cool bathyal waters are the primary site of adaptive radiation in the deep sea, and we hypothesise that bathymetric variation in speciation rates could drive the unimodal diversity-depth pattern over time. Thermal
Full Text Available Braya humilis (Brassicaceae is a widely distributed plant in arid and semi-arid regions of northern Asia. This plant is well adapted to extremely arid conditions and is a promising candidate species to discover novel drought tolerance strategies. However, not much information about the mechanism(s mediating drought resistance in this species is currently available. Therefore, the present study aimed to characterize the physiological traits and expression patterns of a polyethylene glycol (PEG-responsive gene in B. humilis responding to different levels of osmotic stress induced by PEG-6000. Several important physiological parameters were examined, including the levels of relative water content, soluble protein, malondialdehyde, and antioxidant enzyme activity. A tolerance threshold between 20 and 30% PEG-6000 was identified for B. humilis. The water status and oxidative damage below this threshold were maintained at a relatively constant level during the 12 h of treatment. However, once the threshold was exceeded, the water status and oxidative damage were obviously affected after treatment for 4 h. The soluble protein results suggest that B. humilis maintains a vigorous resistance to osmotic stress and that it may play a greater role in osmotic regulation at late stages of stress. Moreover, superoxide dismutase and catalase may be important at preventing oxidative damage in plants at early stages of stress, while peroxidase may be more involved in some biological processes that resist osmotic stress at the late stage, especially in severely damaged plants. Furthermore, a PEG-responsive gene, BhCIPK12, was identified by differential display reverse transcription-polymerase chain reaction (PCR, cloned, and characterized by quantitative real-time PCR. We hypothesized that this gene may play an important role in mediating osmotic stress or drought resistance in plants. Altogether, these results provide valuable insights into the mechanism
Rugh, John P [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Chaney, Larry [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Hepokoski, Mark [ThermoAnalytics Inc.; Curran, Allen [ThermoAnalytics Inc.; Burke, Richard [Measurement Technology NW; Maranville, Clay [Ford Motor Company
Reliable assessment of occupant thermal comfort can be difficult to obtain within automotive environments, especially under transient and asymmetric heating and cooling scenarios. Evaluation of HVAC system performance in terms of comfort commonly requires human subject testing, which may involve multiple repetitions, as well as multiple test subjects. Instrumentation (typically comprised of an array of temperature sensors) is usually only sparsely applied across the human body, significantly reducing the spatial resolution of available test data. Further, since comfort is highly subjective in nature, a single test protocol can yield a wide variation in results which can only be overcome by increasing the number of test replications and subjects. In light of these difficulties, various types of manikins are finding use in automotive testing scenarios. These manikins can act as human surrogates from which local skin and core temperatures can be obtained, which are necessary for accurately predicting local and whole body thermal sensation and comfort using a physiology-based comfort model (e.g., the Berkeley Comfort Model). This paper evaluates two different types of manikins, i) an adaptive sweating thermal manikin, which is coupled with a human thermoregulation model, running in real-time, to obtain realistic skin temperatures; and, ii) a passive sensor manikin, which is used to measure boundary conditions as they would act on a human, from which skin and core temperatures can be predicted using a thermophysiological model. The simulated physiological responses and comfort obtained from both of these manikin-model coupling schemes are compared to those of a human subject within a vehicle cabin compartment transient heat-up scenario.
Hornstrup, Therese; Wikman, Johan Michael; Fristrup, Bjørn
, 38.1 ± 3.7 mL/min/kg). Physiological and psychological and motivational training adaptations were assessed pre- and post-intervention by Dual-Energy X-ray Absorptiometry (DXA) scans, blood sampling, physical tests, and questionnaires. Results: The average heart rate (HR) over all training sessions...... an increase in intrinsic motivation (p fitness, well...
Full Text Available Salinity tolerance has been extensively investigated in recent years due to its agricultural importance. Several features, such as the regulation of ionic transporters and metabolic adjustments, have been identified as salt tolerance hallmarks. Nevertheless, due to the complexity of the trait, the results achieved to date have met with limited success in improving the salt tolerance of rice plants when tested in the field, thus suggesting that a better understanding of the tolerance mechanisms is still required. In this work, differences between two varieties of rice with contrasting salt sensitivities were revealed by the imaging of photosynthetic parameters, ion content analysis and a transcriptomic approach. The transcriptomic analysis conducted on tolerant plants supported the setting up of an adaptive program consisting of sodium distribution preferentially limited to the roots and older leaves, and in the activation of regulatory mechanisms of photosynthesis in the new leaves. As a result, plants resumed grow even under prolonged saline stress. In contrast, in the sensitive variety, RNA-seq analysis revealed a misleading response, ending in senescence and cell death. The physiological response at the cellular level was investigated by measuring the intracellular profile of H2O2 in the roots, using a fluorescent probe. In the roots of tolerant plants, a quick response was observed with an increase in H2O2 production within 5 min after salt treatment. The expression analysis of some of the genes involved in perception, signal transduction and salt stress response confirmed their early induction in the roots of tolerant plants compared to sensitive ones. By inhibiting the synthesis of apoplastic H2O2, a reduction in the expression of these genes was detected. Our results indicate that quick H2O2 signaling in the roots is part of a coordinated response that leads to adaptation instead of senescence in salt-treated rice plants.
Nymo, Siren; Coutinho, Silvia R; Torgersen, Linn-Christin H; Bomo, Ola J; Haugvaldstad, Ingrid; Truby, Helen; Kulseng, Bård; Martins, Catia
Diet-induced weight loss (WL) is associated with reduced resting and non-resting energy expenditure (EE), driven not only by changes in body composition but also potentially by adaptive thermogenesis (AT). When exactly this happens, during progressive WL, remains unknown. The aim of this study was to determine the timeline of changes in RMR and exercise-induced EE (EIEE), stemming from changes in body composition v. the presence of AT, during WL with a very-low-energy diet (VLED). In all, thirty-one adults (eighteen men) with obesity (BMI: 37 (sem 4·5) kg/m2; age: 43 (sem 10) years) underwent 8 weeks of a VLED, followed by 4 weeks of weight maintenance. Body weight and composition, RMR, net EIEE (10, 25 and 50 W) and AT (for RMR (ATRMR) and EIEE (ATEIEE)) were measured at baseline, day 3 (2 (sem 1) % WL), after 5 and 10 % WL and at weeks 9 (16 (sem 2) %) and 13 (16 (sem 1) %). RMR and fat mass were significantly reduced for the first time at 5 % WL (12 (sem 8) d) (P<0·01 and P<0·001, respectively) and EIEE at 10 % WL (32 (sem 8) d), for all levels of power (P<0·05), and sustained up to week 13. ATRMR was transiently present at 10 % WL (-460 (sem 690) kJ/d, P<0·01). A fall in RMR should be anticipated at ≥5 % WL and a reduction in EIEE at ≥10 % WL. Transient ATRMR can be expected at 10 % WL. These physiological adaptations may make progressive WL difficult and will probably contribute to relapse.
Silva, P R B; Machado, K S; Da Silva, D N Lobão; Moraes, J G N; Keisler, D H; Chebel, R C
The aim of this experiment was to determine effects of treating peripartum dairy cows with body condition score ≥3.75 with recombinant bovine somatotropin (rbST) on immune, inflammatory, and metabolic responses. Holstein cows (253±1d of gestation) were assigned randomly to 1 of 3 treatments: untreated control (n=53), rbST87.5 (n=56; 87.5mg of rbST), and rbST125 (n=57; 125mg of rbST). Cows in the rbST87.5 and rbST125 treatments received rbST weekly from -21 to 28d relative to calving. Growth hormone, insulin-like growth factor 1, haptoglobin, tumor necrosis factor α, nonesterified fatty acids, β-hydroxybutyrate, glucose, and cortisol concentrations were determined weekly from -21 to 21d relative to calving. Blood sampled weekly from -14 to 21d relative to calving was used for hemogram and polymorphonuclear leukocyte (PMNL) expression of adhesion molecules, phagocytosis, and oxidative burst. Cows were vaccinated with ovalbumin at -21, -7, and 7d relative to calving, and blood was collected weekly from -21 to 21d relative to calving to determine IgG anti-ovalbumin concentrations. A subsample of cows had liver biopsied -21, -7, and 7d relative to calving to determine total lipids, triglycerides, and glycogen content. Growth hormone concentrations prepartum (control=11.0±1.2, rbST87.5=14.1±1.2, rbST125=15.1±1.3ng/mL) and postpartum (control=14.4±1.1, rbST87.5=17.8±1.2, rbST125=21.8±1.1ng/mL) were highest for rbST125 cows. Cows treated with rbST had higher insulin-like growth factor 1 concentrations than control cows (control=110.5±4.5, rbST87.5=126.2±4.5, rbST125=127.2±4.5ng/mL) only prepartum. Intensity of L-selectin expression was higher for rbST125 than for control and rbST87.5 cows [control=3,590±270, rbST87.5=3,279±271, rbST125=4,371±279 geometric mean fluorescence intensity (GMFI)] in the prepartum period. The PMNL intensities of phagocytosis (control=3,131±130, rbST87.5=3,391±133, rbST125=3,673±137 GMFI) and oxidative burst (control=9,588±746
Irineu Arcaro Júnior
Full Text Available O objetivo deste trabalho foi avaliar a eficiência do uso da climatização no ambiente da sala de espera (ventilação forçada ou ventilação forçada e aspersão sobre as variáveis ambientais e fisiológicas de vacas em lactação. O período experimental teve duração de 90 dias, em que foram avaliadas 21 fêmeas, com produção média de leite de 21kg leite d-1,distribuídas em delineamento inteiramente casualizado. Os tratamentos foram: sala de espera sem climatização (CONTR, sala de espera com ventilação forçada (V e sala de espera com ventilação forçada e aspersão (VA. As variáveis fisiológicas analisadas foram a temperatura retal (TR, freqüência respiratória (FR e temperatura de pele (cabeça, dorso e glândula mamária coletadas antes e depois da aplicação de cada tratamento. As variáveis ambientais registradas foram a temperatura de bulbo seco (TBS, temperatura de globo negro (TGN e umidade relativa (UR, coletadas antes e depois da aplicação de cada tratamento. O tratamento VA foi mais eficiente em reduzir a TBS (6,4°C e TGN (6,5°C. Os tratamentos V e VA diminuíram significativamente a FR dos animais. A variável temperatura de pele apresentou redução de 4,2°C para a região da cabeça e 2,8°C para a região do dorso, no tratamento VA. O tratamento VA proporcionou maiores reduções nas variáveis ambientais e fisiológicas resultando em melhores condições de conforto aos animais e maior eficácia na dissipação de calor pela pele dos animais.The aim of this study was to evaluate the effects of climatization (ventilation or ventilation plus sprinkler inside a holding pen in a milking parlor on environmental and physiological parameters. The experiment was conducted from August to October during 90 consecutive days. Twenty-one lactating dairy cows with an average milk yield of 21kg.d-1 were randomly allocated in three treatments. The treatments were: holding pen without cooling (control; holding pen
Full Text Available A study was conducted to understand the host-parasite relationship in terms of anatomical and physiological adaptations in Morinda tinctoria Roxb. and Cassytha filiformis L. Anatomically the haustorium of Cassytha is found to have two parts, the upper haustorium and the endophyte. The former is the portion of a haustorium that lies external to the host organ, whereas the endophyte is the portion of a haustorium that penetrates host tissues. It was also observed that the host organ triggers the dedifferentiation of cortical parenchyma to develop dense cytoplasm, conspicuous nuclei and numerous starch grains and these cells are found to serve as the initials of upper haustorium. The level of Chl b was lower than Chl a and xanthophylls in Cassytha when compared to Morinda. The photosynthetic activity was measured in intact leaves/stems of both the host and parasitic plant using Chl a fluorescence induction kinetics, which revealed that the photosynthetic efficiency was very low in the infected sample as well as in the parasite stem. Over all, the reduction in the photosynthetic efficiency was correlated to the poorly developed PS II complex.
Carlson, Jane E; Adams, Christopher A; Holsinger, Kent E
Trait-environment relationships are commonly interpreted as evidence for local adaptation in plants. However, even when selection analyses support this interpretation, the mechanisms underlying differential benefits are often unknown. This study addresses this gap in knowledge using the broadly distributed South African shrub Protea repens. Specifically, the study examines whether broad-scale patterns of trait variation are consistent with spatial differences in selection and ecophysiology in the wild. In a common garden study of plants sourced from 19 populations, associations were measured between five morphological traits and three axes describing source climates. Trait-trait and trait-environment associations were analysed in a multi-response model. Within two focal populations in the wild, selection and path analyses were used to test associations between traits, fecundity and physiological performance. Across 19 populations in a common garden, stomatal density increased with the source population's mean annual temperature and decreased with its average amount of rainfall in midsummer. Concordantly, selection analysis in two natural populations revealed positive selection on stomatal density at the hotter, drier site, while failing to detect selection at the cooler, moister site. Dry-site plants with high stomatal density also had higher stomatal conductances, cooler leaf temperatures and higher light-saturated photosynthetic rates than those with low stomatal density, but no such relationships were present among wet-site plants. Leaf area, stomatal pore index and specific leaf area in the garden also co-varied with climate, but within-population differences were not associated with fitness in either wild population. The parallel patterns of broad-scale variation, differences in selection and differences in trait-ecophysiology relationships suggest a mechanism for adaptive differentiation in stomatal density. Densely packed stomata may improve performance by
Carter, Jason R.; West, John B.
Compare and contrast strategies remain common pedagogical practices within physiological education. With the support of an American Physiological Society Teaching Career Enhancement Award, we have developed a junior- or senior-level undergraduate curriculum for exercise physiology that compares and contrasts the physiological adaptations of…
Preliminary biological screening of microbes isolated from cow dung in Kampar. KC Teo, SM Teoh. Abstract. Five distinct morphologically and physiologically isolates were isolated from cow dung at Kampar, Perak, Malaysia and cultured on nutrient agar (NA) plates. Morphological studies including microscopic examination ...
Flávio Baccari Jr
High producing dairy cows are more sensitive to heat stress due mainly to their higher resting metabolic rate as compared to low producing and dry cows. Their responses to increasing levels of the temperature-humidity and the black globe-humidity indices are discussed as well as some aspects of heat tolerance as related to body temperature increase and milk production decrease. Some mitigation and adaptation practices are recommended to face the challenges of global climate changes.
Pang, Jiayin; Yang, Jiyun; Lambers, Hans; Tibbett, Mark; Siddique, Kadambot H M; Ryan, Megan H
The aim of this study was to investigate the capacity of three perennial legume species to access sources of varyingly soluble phosphorus (P) and their associated morphological and physiological adaptations. Two Australian native legumes with pasture potential (Cullen australasicum and Kennedia prostrata) and Medicago sativa cv. SARDI 10 were grown in sand under two P levels (6 and 40 µg P g(-1) ) supplied as Ca(H2 PO4 )2 ·H2 O (Ca-P, highly soluble, used in many fertilizers) or as one of three sparingly soluble forms: Ca10 (OH)2 (PO4 )6 (apatite-P, found in relatively young soils; major constituent of rock phosphate), C6 H6 O24 P6 Na12 (inositol-P, the most common form of organic P in soil) and FePO4 (Fe-P, a poorly-available inorganic source of P). All species grew well with soluble P. When 6 µg P g(-1) was supplied as sparingly soluble P, plant dry weight (DW) and P uptake were very low for C. australasicum and M. sativa (0.1-0.4 g DW) with the exception of M. sativa supplied with apatite-P (1.5 g). In contrast, K. prostrata grew well with inositol-P (1.0 g) and Fe-P (0.7 g), and even better with apatite-P (1.7 g), similar to that with Ca-P (1.9 g). Phosphorus uptake at 6 µg P g(-1) was highly correlated with total root length, total rhizosphere carboxylate content and total rhizosphere acid phosphatase (EC 22.214.171.124) activity. These findings provide strong indications that there are opportunities to utilize local Australian legumes in low P pasture systems to access sparingly soluble soil P and increase perennial legume productivity, diversity and sustainability. © 2014 Scandinavian Plant Physiology Society.
Heterosis and direct effects for Charolais-sired calf weight and growth, cow weight and weight change, and ratios of cow and calf weights and weight changes across warm season lactation in Romosinuano, Angus, and F cows in Arkansas.
Riley, D G; Burke, J M; Chase, C C; Coleman, S W
The use of Brahman in cow-calf production offers some adaptation to the harsh characteristics of endophyte-infected tall fescue. Criollo breeds, such as the Romosinuano, may have similar adaptation. The objectives were to estimate genetic effects in Romosinuano, Angus, and crossbred cows for their weights, weights of their calves, and ratios (calf weight:cow weight and cow weight change:calf weight gain) across lactation and to assess the influence of forage on traits and estimates. Cows ( = 91) were bred to Charolais bulls after their second parity. Calves ( = 214) were born from 2006 to 2009. Cows and calves were weighed in early (April and June), mid- (July), and late lactation (August and October). Animal was a random effect in analyses of calf data; sire was random in analyses of cow records and ratios. Fixed effects investigated included calf age, calf sex, cow age-year combinations, sire breed of cow, dam breed of cow, and interactions. Subsequent analyses evaluated the effect of forage grazed: endophyte-free or endophyte-infected tall fescue. Estimates of maternal heterosis for calf weight ranged from 9.3 ± 4.3 to 15.4 ± 5.7 kg from mid-lactation through weaning ( cow) were -6.8 ± 3.0 and -8.9 ± 4.2 kg for weights recorded in April and June. Calf weights and weight gains from birth were greater ( cows grazing endophyte-free tall fescue except in mid-summer. Cow weight change from April to each time was negative for Angus cows and lower ( Cows grazing endophyte-free tall fescue were heavier ( cows had the lowest ( cow weight change:calf weight gain, indicating an energy-deficit condition. Cows grazing endophyte-free tall fescue had more negative ( cow weight, 7.9 ± 3.0 to 15.8 ± 5.0 kg for cow weight change, and 0.07 ± 0.03 to 0.27 ± 0.1 for cow weight change:calf weight gain. Direct Romosinuano effects ranged from 14.8 ± 4.2 to 49.8 ± 7.7 kg for cow weight change and 0.2 ± 0.04 to 0.51 ± 0.14 for cow weight change:calf weight gain. The adaptive
Amaro, Alexandre Slowetzky; Teixeira, Maria Cristina Triguero Veloz; de Mesquita, Maria Luiza Guedes; Rodrigues, Graciele Massoli; Rubin, Daniela Andrea; Carreiro, Luiz Renato Rodrigues
Physical activity programs are a powerful tool against several diseases including obesity and their comorbidities. Prader-Willi syndrome is the most common genetic disease associated with obesity, and brings with it behavioral and emotional problems that need complex management. Research into the effect of physical activity programs on Prader-Willi syndrome is limited and it is frequently argued that if a physical activity program is too complex, the participants are more likely to drop out. Therefore, in this study, we assessed the physiological adaptation effect of a physical activity program with increasing complexity and load, in a boy and a girl with Prader-Willi syndrome by assessing changes in lipid profile, body composition, and physical fitness parameters. Case 1 was an 11-year-old girl, mixed race (brown), with an intelligence quotient of 68, 52.0 % body fat, and a body mass index of 45.3 kg/m(2). The Prader-Willi syndrome diagnosis was made when she was 5-years old and was found to be due to an imprinting genomic defect. Case 2 was a 14-year-old boy, mixed race (brown), with an intelligence quotient of 74, 48.8 % body fat, and a body mass index of 37.3 kg/m(2). The diagnosis was made when he was 10-years old and was found to be caused by gene deletion. Both participants presented physical characteristics and behavior problems typical of Prader-Willi syndrome. Case 2 presented high blood pressure, high cholesterol and sleep apnea and had to use continuous positive airway pressure to sleep. Both participants were assessed for 12 weeks (three times a week) using a physical activity program designed to improve strength and muscle hypertrophy. The work load was progressively adjusted as necessary and new exercises were added to the program. Prior to the program, the participants' parents received instructions about managing problem behavior and advice about nutrition. After physical activity program several health markers assessed by biological tests and
efficient compensation of pH disturbances during exposure to elevated environmental pCO2. Compensation of extracellular acid-base status in turn may be important in avoiding metabolic depression. So far, maintained "performance" at higher seawater pCO2 (>0.3 to 0.6 kPa has only been observed in adults/juveniles of active, high metabolic species with a powerful ion regulatory apparatus. However, while some of these taxa are adapted to cope with elevated pCO2 during their regular embryonic development, gametes, zygotes and early embryonic stages, which lack specialized ion-regulatory epithelia, may be the true bottleneck for ecological success – even of the more tolerant taxa. Our current understanding of which marine animal taxa will be affected adversely in their physiological and ecological fitness by projected scenarios of anthropogenic ocean acidification is quite incomplete. While a growing amount of empirical evidence from CO2 perturbation experiments suggests that several taxa might react quite sensitively to ocean acidification, others seem to be surprisingly tolerant. However, there is little mechanistic understanding on what physiological traits are responsible for the observed differential sensitivities (see reviews of Seibel and Walsh, 2003; Pörtner et al., 2004; Fabry et al., 2008; Pörtner, 2008. This leads us to the first very basic question of how to define general CO2 tolerance on the species level.
Safarimosavi, Saleh; Mohebbi, Hamid; Rohani, Hadi
Safarimosavi, S, Mohebbi, H, and Rohani, H. High-intensity interval vs. continuous endurance training: Preventive effects on hormonal changes and physiological adaptations in prediabetes patients. J Strength Cond Res XX(X): 000-000, 2018-The aim of this study was to examine the effects of a 12-week high-intensity interval training (HIIT) intervention, or an isocaloric continuous endurance training (CET) intervention on insulin resistance indices and change in irisin and preptin in patients with prediabetes. Thirty-two prediabetic male patients (age = 38.7 ± 4; body mass index = 26.9 ± 1.4 kg·m; and V[Combining Dot Above]O2peak = 2.49 ± 0.22 L·min) were randomly assigned into 3 training groups (N = 8). These groups were matched based on the required energy expenditure (EE) for completing each protocol: (a) HIIT (10 × 60 seconds at 90% peak oxygen uptake [V[Combining Dot Above]O2peak], 1: 1 work to recovery at 50 W), (b) CET at an intensity equivalent to maximal fat oxidation (Fatmax) (CETFAT) (pedaling for a duration that expends an equivalent EE to an HIIT session [E ≈ HIIT]), (c) CET at an intensity equivalent to anaerobic threshold (CETAT) (E ≈ HIIT), and (d) the control group (CON): continued to perform their daily activities. After intervention, blood glucose levels were significantly (p HIIT group compared with CETAT group. Exercise training improved the insulin resistance index by 35, 28, and 37% in CETFAT, CETAT, and HIIT groups, respectively. Irisin concentrations in the HIIT and CETAT groups was significantly (p HIIT and CETFAT resulted in significant (p HIIT and CETFAT protocols had similar effects on the insulin resistance index of prediabetic patients. Also, the intensity and type of exercise were effective factors in changing irisin and preptin concentrations.
Dennis F A E Voeten
brain shape and accessory organs were achieved through heterochronic development of the cranium, particularly the braincase. In summary, the cranium of Nothosaurus marchicus reflects important physiological and neurosensory adaptations that enabled the group's explosive invasion of shallow marine habitats in the late Early Triassic.
... Safe Videos for Educators Search English Español Mad Cow Disease KidsHealth / For Parents / Mad Cow Disease What's ... Is Being Done About It Print About Mad Cow Disease Mad cow disease has been in the ...
Jin Wook Kim
Full Text Available This review focuses on modulation of growth hormone (GH and its downstream actions on periparturient dairy cows undergoing physiological and metabolic adaptations. During the periparturient period, cows experience a negative energy balance implicating that the feed intake does not meet the total energy demand for the onset of lactation. To regulate this metabolic condition, key hormones of somatotropic axis such as GH, IGF-I and insulin must coordinate adaptations required for the preservation of metabolic homeostasis. The hepatic GHR1A transcript and GHR protein are reduced at parturition, but recovers on postpartum. However, plasma IGF-I concentration remains low even though hepatic abundance of the GHR and IGF-I mRNA return to pre-calving value. This might be caused by alternation in IGFBPs and ALS genes, which consequently affect the plasma IGF-I stability. Plasma insulin level declines in a parallel manner with the decrease in plasma IGF-I after parturition. Increased GH stimulates the lipolytic effects and hepatic glucose synthesis to meet the energy requirement for mammary lactose synthesis, suggesting that GH antagonizes insulin-dependent glucose uptake and attenuates insulin action to decrease gluconeogenesis.
Friggens, Nic; Disenhaus, C; Petit, H V
for the dairy producer. In this study we focus on understanding the overall biological phenomena associated with nutritional sub-fertility rather than the underlying multiplicity of physiological interactions (already described in a number of recent studies). These phenomena are important because they represent...... the natural adaptations of the animal for dealing with variations in the nutritional environment. They can also be used to monitor and modulate reproductive performance on-farm. There is an underlying trade-off between two aspects of reproduction: investment in the viability of the current calf and investment...... embryos, which are of poorer quality, than do non-lactating cows. The same applies to high- compared to medium-genetic merit cows. Another important biological property is the adaptive use of body reserves in support of reproduction. Orchestrated endocrine changes in pregnancy and lactation facilitate...
In the dairy industry, udder health is associated with mastitis management, of which blanket dry cow treatment has been an important part for decades. To prevent the udder from new intramammary infections during the dry period, the use of blanket dry cow treatment has been advocated for more than 50 years as part of the five-point mastitis prevention program. The goal of dry cow treatment is to reduce the prevalence of intramammary infections by eliminating infections already present at dryin...
KANDEMİR, GAYE; ÖNDE, SERTAÇ; TEMEL, FATİH; KAYA, ZEKİ
Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to ...
The role of oxidative, inflammatory and neuroendocrinological systems during exercise stress in athletes: implications of antioxidant supplementation on physiological adaptation during intensified physical training.
Slattery, Katie; Bentley, David; Coutts, Aaron J
During periods of intensified physical training, reactive oxygen species (ROS) release may exceed the protective capacity of the antioxidant system and lead to dysregulation within the inflammatory and neuroendocrinological systems. Consequently, the efficacy of exogenous antioxidant supplementation to maintain the oxidative balance in states of exercise stress has been widely investigated. The aim of this review was to (1) collate the findings of prior research on the effect of intensive physical training on oxidant-antioxidant balance; (2) summarise the influence of antioxidant supplementation on the reduction-oxidation signalling pathways involved in physiological adaptation; and (3) provide a synopsis on the interactions between the oxidative, inflammatory and neuroendocrinological response to exercise stimuli. Based on prior research, it is evident that ROS are an underlying aetiology in the adaptive process; however, the impact of antioxidant supplementation on physiological adaptation remains unclear. Equivocal results have been reported on the impact of antioxidant supplementation on exercise-induced gene expression. Further research is required to establish whether the interference of antioxidant supplementation consistently observed in animal-based and in vivo research extends to a practical sports setting. Moreover, the varied results reported within the literature may be due to the hormetic response of oxidative, inflammatory and neuroendocrinological systems to an exercise stimulus. The collective findings suggest that intensified physical training places substantial stress on the body, which can manifest as an adaptive or maladaptive physiological response. Additional research is required to determine the efficacy of antioxidant supplementation to minimise exercise-stress during intensive training and promote an adaptive state.
Pérez-Ramírez, E; Peyraud, J L; Delagarde, R
In pasture-based dairy systems, daily time at pasture is restricted during several periods of the year. The aim of this experiment was to evaluate the effect of restricting time at pasture on milk yield, pasture dry matter (DM) intake, and grazing behavior of dairy cows according to pasture allowance (PA), which partly defines pasture availability. The experiment was carried out in spring on strip-grazed perennial ryegrass pastures. The 6 treatments consisted of 3 durations of daily time at pasture [U: unrestricted day and night grazing (22 h at pasture); R9: 1 grazing session restricted to 9 h between the 2 milkings; R5: 2 grazing sessions of 2.75 h after each milking) compared at low and high PA (13 and 24 kg of DM/d per cow >5 cm, respectively). Eighteen mid-lactation Holstein dairy cows were used according to a 6 x 4 incomplete Latin square design replicated 3 times with four 14-d periods. Pasture DM intake was measured by the ytterbium-fecal index method and grazing behavior from portable devices. On average, restricting time at pasture from U to R (mean of R5 + R9) decreased pasture intake by 2.9 kg of DM, milk yield by 1.3 kg, and milk protein concentration by 0.11%, and increased milk fat concentration by 0.20%. Pasture intake and milk yield did not differ significantly between R9 and R5. The reduction of pasture intake and milk yield with decreasing time at pasture was greater at high compared with low PA. Grazing times were 536, 414, and 305 min, representing proportions of time spent grazing of 0.40, 0.77, and 0.93 for treatments U, R9, and R5, respectively. The reduction of grazing time with decreasing time at pasture was greater at high compared with low PA. Pasture intake rate greatly increased with decreasing time at pasture, but mainly on R5 (29.8, 31.6, and 42.1 g of DM/min for U, R9, and R5, respectively). The effect of time at pasture on pasture intake rate was unaffected by PA. In conclusion, the effect of restriction of time at pasture on
... this page: //medlineplus.gov/ency/article/002448.htm Cow's milk - infants To use the sharing features on ... year old, you should not feed your baby cow's milk, according to the American Academy of Pediatrics ( ...
In the dairy industry, udder health is associated with mastitis management, of which blanket dry cow treatment has been an important part for decades. To prevent the udder from new intramammary infections during the dry period, the use of blanket dry cow treatment has been advocated for more than 50
Full text: The rumen is an important part of the digestive tract of ruminant animals such as cattle, buffalo, sheep and goats. It contains large numbers of micro-organisms whose function is to break down fibrous feed materials such as grass and straw and convert them to products that can be used by the animal to produce meat, milk, wool or draught power. To study the microbial population of the rumen under controlled laboratory conditions, Dr. J.W. Czerkawski of the Hannah Research Institute, Scotland, U.K., developed an 'artificial cow'. The 'cow', named RUSITEC (from the acronym of 'Rumen Simulation Technique') is today being used as part of a project to analyse different feedstuffs being carried out by the Food and Agriculture Organization of the United Nations (FAO) and the International Atomic Energy Agency (IAEA) at their joint Agricultural Laboratory at Seibersdorf near Vienna, Austria. In the artificial rumen micro-organisms can be indefinitely maintained by feeding a normal ruminant diet each day and providing the correct physiological conditions in terms of temperature, pH and flow of saliva. As RUSITEC chews its way through different feeds, scientists use radioactive tracing techniques to compare their digestibility. (The higher the digestibility of a foodstuff, the higher the nutritive value that can be derived from it.) By analysing the quality of different feeding materials in this way, scientists are seeking to propose improved diets for domestic animals in the developing world. Photos on this page show RUSITEC at work. Below, the vessels representing the rumen, where microbial fermentation of diets takes place; right, the rumen simulation technique in operation; below right, analysis of the end products of fermentative digestion
ENRICO L. REZENDE
Full Text Available How complex physiological systems evolve is one of the major questions in evolutionary physiology. For example, how traits interact at the physiological and genetic level, what are the roles of development and plasticity in Darwinian evolution, and eventually how physiological traits will evolve, remains poorly understood. In this article we summarize the current frame of work evolutionary physiologists are employing to study the evolution of physiological adaptations, as well as the role of developmental and reversible phenotypic plasticity in this context. We also highlight representative examples of how the integration of evolutionary and developmental physiology, concomitantly with the mechanistic understanding of physiological systems, can provide a deeper insight on how endothermic vertebrates could cope with reduced ambient temperatures and oxygen availability characteristic of high altitude environments. In this context, high altitude offers a unique system to study the evolution of physiological traits, and we believe much can be gained by integrating theoretical and empirical knowledge from evolutionary biology, such as life-history theory or the comparative method, with the mechanistic understanding of physiological processesUna de las preguntas más importantes en fisiología evolutiva es como evolucionan los sistemas fisiológicos complejos. Por ejemplo, actualmente sabemos poco sobre la interacción entre varios rasgos a niveles genéticos y fisiológicos, sobre el papel de la plasticidad fenotípica durante distintas etapas del desarrollo y madurez para la evolución fisiológica dentro de un linaje. En este trabajo explicamos el marco conceptual ocupado por fisiólogos evolutivos en la actualidad para estudiar adaptaciones fisiológicas a nivel evolutivo y el papel de la plasticidad dentro de la evolución Darviniana. Citamos ejemplos de como la integración de la fisiología evolutiva y del desarrollo nos permitió un mayor
building skills, knowledge or networks on adaptation, ... the African partners leading the AfricaAdapt network, together with the UK-based Institute of Development Studies; and ... UNCCD Secretariat, Regional Coordination Unit for Africa, Tunis, Tunisia .... 26 Rural–urban Cooperation on Water Management in the Context of.
Katiyatiya, C. L. F.; Muchenje, V.
Breed, age, coat colour, month and temperature humidity index (THI) influence on body weight (W), body condition score (BCS), thermophysiological variables (rectal temperature (Tr), skin temperature (Tsk)) and hair length was studied in Nguni ( n = 19) and Boran ( n = 16). As a result of this study, breed influenced W, BCS and Tsk on the neck and belly ( P cows were higher than the Boran cows. Hair length of both breeds increased from February to August. The THI influenced thermophysiological variables ( P cows (3-8 years) had lower weight and high Tr and Tsk ( P cows had high neck and thurl temperatures in June while Boran cows had the highest in August ( P cows had increased BCS. Nguni cows with red, fawn and white cows had high BCS. Fawn-coloured Nguni cows and white-brown Boran cows had the more weight than cows with other colours compared in the study. White-red Nguni and Boran cows recorded the highest Tr. For Nguni cows, neck and belly temperatures were significantly ( P cows had significant ( P cows were more adapted to the prevailing bioclimatic changes. However, Boran cows have the potential of performing well under heat stress conditions over time.
Friggens, N C; Disenhaus, C; Petit, H V
There has been a significant decline in the reproductive performance of dairy cattle in recent decades. Cows, take longer time to return to the oestrus after calving, have poorer conception rates, and show fewer signs of oestrus. Achieving good reproductive performance is an increasing challenge for the dairy producer. In this study we focus on understanding the overall biological phenomena associated with nutritional sub-fertility rather than the underlying multiplicity of physiological interactions (already described in a number of recent studies). These phenomena are important because they represent the natural adaptations of the animal for dealing with variations in the nutritional environment. They can also be used to monitor and modulate reproductive performance on-farm. There is an underlying trade-off between two aspects of reproduction: investment in the viability of the current calf and investment in future offspring. As the investment in, and viability of, the current calf is related to maternal milk production, we can expect that level of milk production per se has effects on subsequent reproductive performance (investment in future offspring). Lactating cows have a lower proportion of viable embryos, which are of poorer quality, than do non-lactating cows. The same applies to high- compared to medium-genetic merit cows. Another important biological property is the adaptive use of body reserves in support of reproduction. Orchestrated endocrine changes in pregnancy and lactation facilitate the deposition of body lipid during pregnancy and mobilisation in early lactation. When the cow fails to accumulate the reserves she needs to safeguard reproduction she delays committing to further reproductive investment. But how does the cow 'know' that she is failing in energy terms? We argue that the cow does this by 'monitoring' both the body fat mobilisation and body fatness. Excessive body fat mobilisation indicates that current conditions are worse than
Derous, D.; Kelder, T.; Schothorst, E.M. van; Erk, M. van; Voigt, A.; Klaus, S.; Keijer, J.; Radonjic, M.
Health is influenced by interplay of molecular, physiological and environmental factors. To effectively maintain health and prevent disease, health-relevant relations need to be understood at multiple levels of biological complexity. Network-based methods provide a powerful platform for integration
Nelson, Regina K.
A mixed-methods research study was designed to test whether undergraduate engineering students were better prepared to learn advanced topics in biomedical engineering if they learned physiology via a quantitative, concept-based approach rather than a qualitative, system-based approach. Experiments were conducted with undergraduate engineering…
Bugental, Daphne Blunt
Children's physiological reactions to stress are presented from the broader theoretical perspective of adaptive calibration to the environment, as rooted in life history theory. Del Giudice, Hinnant, Ellis, and El-Sheikh (2012) focus on children's physiological responses to a stressful task as a consequence of their history of family stress.…
Full Text Available Abstract Impairment of the insulin regulation of energy metabolism is considered to be an etiologic key component for metabolic disturbances. Methods for studies of insulin sensitivity thus are highly topical. There are clear indications that reduced insulin sensitivity contributes to the metabolic disturbances that occurs especially among obese lactating cows. Direct measurements of insulin sensitivity are laborious and not suitable for epidemiological studies. We have therefore adopted an indirect method originally developed for humans to estimate insulin sensitivity in dairy cows. The method, "Revised Quantitative Insulin Sensitivity Check Index" (RQUICKI is based on plasma concentrations of glucose, insulin and free fatty acids (FFA and it generates good and linear correlations with different estimates of insulin sensitivity in human populations. We hypothesized that the RQUICKI method could be used as an index of insulin function in lactating dairy cows. We calculated RQUICKI in 237 apparently healthy dairy cows from 20 commercial herds. All cows included were in their first 15 weeks of lactation. RQUICKI was not affected by the homeorhetic adaptations in energy metabolism that occurred during the first 15 weeks of lactation. In a cohort of 24 experimental cows fed in order to obtain different body condition at parturition RQUICKI was lower in early lactation in cows with a high body condition score suggesting disturbed insulin function in obese cows. The results indicate that RQUICKI might be used to identify lactating cows with disturbed insulin function.
Lori Marino; Kristin Allen
Domestic cows (Bos taurus) are consumed worldwide as beef and veal, kept as dairy product producers, employed as draft animals in labor, and are used for a long list of other products, including leather and manure. But despite global reliance on cows for thousands of years, most people’s perception of them is as plodding herd animals with little individual personality and very simple social relationships or preferences. Yet, a review of the scientific literature on cow behavior points to more...
Bargatze, L. F.
Active Data Archive Product Tracking (ADAPT) is a collection of software routines that permits one to generate XML metadata files to describe and register data products in support of the NASA Heliophysics Virtual Observatory VxO effort. ADAPT is also a philosophy. The ADAPT concept is to use any and all available metadata associated with scientific data to produce XML metadata descriptions in a consistent, uniform, and organized fashion to provide blanket access to the full complement of data stored on a targeted data server. In this poster, we present an application of ADAPT to describe all of the data products that are stored by using the Common Data File (CDF) format served out by the CDAWEB and SPDF data servers hosted at the NASA Goddard Space Flight Center. These data servers are the primary repositories for NASA Heliophysics data. For this purpose, the ADAPT routines have been used to generate data resource descriptions by using an XML schema named Space Physics Archive, Search, and Extract (SPASE). SPASE is the designated standard for documenting Heliophysics data products, as adopted by the Heliophysics Data and Model Consortium. The set of SPASE XML resource descriptions produced by ADAPT includes high-level descriptions of numerical data products, display data products, or catalogs and also includes low-level "Granule" descriptions. A SPASE Granule is effectively a universal access metadata resource; a Granule associates an individual data file (e.g. a CDF file) with a "parent" high-level data resource description, assigns a resource identifier to the file, and lists the corresponding assess URL(s). The CDAWEB and SPDF file systems were queried to provide the input required by the ADAPT software to create an initial set of SPASE metadata resource descriptions. Then, the CDAWEB and SPDF data repositories were queried subsequently on a nightly basis and the CDF file lists were checked for any changes such as the occurrence of new, modified, or deleted
The COW, or Console On Wheels, is the primary operator interface to the SLC accelerator control system. A hardware and software description of the COW, a microcomputer based system with a color graphics display output and touch-panel and knob inputs, is given. The ease of development and expandability, due to both the modular nature of the hardware and the multitasking, interrupt driven software running in the COW, are described. Integration of the COW into the SLCNET communications network and SLC Control system is detailed
The COW, or Console On Wheels, is the primary operator interface to the SLC accelerator control system. A hardware and software description of the COW, a microcomputer based system with a color graphics display output and touchpanel and knob inputs, is given. The ease of development and expandability, due to both the modular nature of the hardware and the multitasking, interrupt driven software running in the COW, are described. Integration of the COW into the SLCNET communications network and SLC Control system is detailed
Allemand, Denis; Furla, Paola
Cnidarians (corals and sea anemones) harbouring photosynthetic microalgae derive several benefits from their association. To allow this association, numerous symbiotic-dependent adaptations in both partners, resulting from evolutionary pressures, have been selected. The dinoflagellate symbionts (zooxanthellae) are located inside a vesicle in the cnidarian host cell and are therefore exposed to a very different environment compared to the free-living state of these microalgae in terms of ion concentration and carbon content and speciation. In addition, this intracellular localization imposes that they rely completely upon the host for their nutrient supply (nitrogen, CO 2 ). Symbiotic-dependent adaptations imposed to the animal host by phototrophic symbiosis are more relevant to photosynthetic organisms than to metazoans: indeed, the cnidarian host often harbours diurnal changes of morphology to adapt itself to the amount of light and possesses carbon-concentrating mechanisms, antioxidative defences and UV sunscreens similar to that present in phototrophs. These adaptations and the contrasting fragility of the association are discussed from both ecological and evolutionary points of view. Copyright © 2018 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.
Schuback, N.; Hippmann, A.; Maldonado, M. T.; Allen, A. E.; McCrow, J.; Foster, L. J.; Green, B. R.; Alami, M.
Iron plays a significant role in controlling marine primary productivity. Despite that extremely low dissolved iron (Fe) concentrations are found in Fe-limited regions, some phytoplankton are able to survive and thrive. Two strains of the model oceanic diatom Thalassiosira oceanica, TO 1003 and TO 1005, have both been used in previous studies to characterize adaptations to iron limitation. These studies have shown that T. oceanica has lowered its Fe requirements and increased its Fe acquisition efficiency compared to coastal counterparts. Both strategies may impose a higher cellular copper (Cu) demand. However, the underlying biochemical adaptations in these oceanic diatoms remain unknown. Recently, the genome, as well as the first proteomic and transcriptomic analyses of T. oceanica 1005 grown under different Fe levels, were published. To further our understanding of the interplay between Fe- and Cu- physiology in open ocean diatoms, we examined an array of physiological responses to varying degrees of Fe-, Cu- and Fe/Cu co-limitation in both strains. We also determined the differential expression of proteins using stable isotope labeling and LC-MS/MS proteomic analysis. The two strains, TO 1003 and TO 1005, need markedly different metal concentrations in the media. TO1003 requires 30% less Cu to sustain its optimal growth and less than 1/10th of the minimum Cu that is needed by TO 1005 to survive. In contrast, TO 1005 is able to grow with less Fe available in the media. The physiological and proteomic responses of these two strains when acclimated to low Fe and/or Cu concentrations will be presented. The evolutionary implications will be discussed.
Kletkiewicz, H; Rogalska, J; Nowakowska, A; Wozniak, A; Mila-Kierzenkowska, C; Caputa, M
It is well known that decrease in body temperature provides protection to newborns subjected to anoxia/ischemia. We hypothesized that the normal body temperature of 33°C in neonatal rats (4°C below normal body temperature in adults) is in fact a preadaptation to protect CNS from anoxia and further reductions as well as elevations in temperature may be counterproductive. Our experiments aimed to examine the effect of changes in body temperature on oxidative stress development in newborn rats exposed to anoxia. Two-day-old Wistar rats were divided into 4 temperature groups: i. hypothermic at body temperature of 31°C, ii. maintaining physiological neonatal body temperature of 33°C, iii. forced to maintain hyperthermic temperature of 37°C, and i.v. forced to maintain hyperthermic temperature of 39°C. The temperature was controlled starting 15 minutes before and afterword during 10 minutes of anoxia as well as for 2 hours post-anoxia. Cerebral concentrations of lipid peroxidation products malondialdehyde (MDA) and conjugated dienes (CD) and the activities of antioxidant enzymes had been determined post mortem: immediately after anoxia was finished and 3, 7, and 14 days later. There were no post-anoxic changes in the concentration of MDA, CD and in antioxidant enzymes activity in newborn rats kept at their physiological body temperature of 33°C. In contrast, perinatal anoxia at body temperature elevated to 37°C or 39°C as well as under hypothermic conditions (31°C) intensified post-anoxic oxidative stress and depleted the antioxidant pool. Overall, these findings suggest that elevated body temperature (hyperthermia or fever), as well as exceeding cooling beyond the physiological level of body temperature of newborn rats, may extend perinatal anoxia-induced brain lesions. Our findings provide new insights into the role of body temperature in anoxic insult in vivo.
Sejian, Veerasamy; Maurya, Vijai P.; Naqvi, Syed M. K.
Sheep in hot semi-arid environments are mostly reared using extensive systems. In addition to thermal stress and feed scarcity, the animals need to walk long distances for grazing in this ecological zone. A study was conducted to assess the effect of long-distance walking on adaptive capability in terms of physiological, biochemical and endocrine responses in Malpura ewes. Fourteen adult Malpura non-pregnant ewes weighing between 33 and 35 kg were used in the study. The ewes were randomly allocated into two groups of seven animals each: GI ( n = 7; Control), and GII ( n = 7; walking stress). The animals were stall-fed with a diet consisting of 70% roughage and 30% concentrate. Both GI and GII ewes had uniform access to feed and water. The walking stress group (GII) ewes were made to walk 14 km in two spans between 0900 and 1500 hours with 1 h 30 min for each span (7 km) of walking. The ewes subjected to walking stress (GII) were prevented from grazing by applying a face mask made of cotton thread. The study was conducted for a period of two estrous cycles (35 days) during the autumn season (October-November). Physiological responses were recorded twice daily at 0800 and 1400 hours at weekly intervals. Blood samples were collected from the jugular vein at weekly intervals to study the effects of walking stress on blood biochemical and endocrine parameters. The results indicate that walking stress had significant ( P ewes have the capability to adapt to long-distance walking, and that adrenal and thyroid gland hormones play a significant role in such adaptation.
Laura A Thompson
Full Text Available Increased pressure, associated with diving, can alter cell function through several mechanisms and has been shown to impact immune functions performed by peripheral blood mononuclear cells (PBMC in humans. While marine mammals possess specific adaptations which protect them from dive related injury, it is unknown how their immune system is adapted to the challenges associated with diving. The purpose of this study was to measure PBMC activation (IL2R expression and Concanavalin A induced lymphocyte proliferation (BrdU incorporation in belugas following in vitro pressure exposures during baseline, Out of Water Examination (OWE and capture/release conditions. Beluga blood samples (n=4 were obtained from animals at the Mystic Aquarium and from free ranging animals in Alaska (n=9. Human blood samples (n=4 (Biological Specialty Corporation were run for comparison. In vivo catecholamines and cortisol were measured in belugas to characterize the neuroendocrine response. Comparison of cellular responses between controls and pressure exposed cells, between conditions in belugas, between belugas and humans as well as between dive profiles, were run using mixed generalized linear models (α=0.05. Cortisol was significantly higher in wild belugas and OWE samples as compared with baseline for aquarium animals. Both IL2R expression and proliferation displayed significant pressure induced changes, and these responses varied between conditions in belugas. Both belugas and humans displayed increased IL2R expression, while lymphocyte proliferation decreased for aquarium animals and increased for humans and wild belugas. Results suggest beluga PBMC function is altered during diving and changes may represent dive adaptation as the response differs from humans, a non-dive adapted mammal. In addition, characteristics of a dive (i.e., duration, depth as well as neuroendocrine activity can alter the response of beluga cells, potentially impacting the ability of animals
Prinzel, Lawrence J., III; Pope, Alan T.; Freeman, Frederick G.
Prinzel, Hadley, Freeman, and Mikulka found that adaptive task allocation significantly enhanced performance only when used at the endpoints of the task workload continuum (i.e., very low or high workload), but that the technique degraded performance if invoked during other levels of task demand. These researchers suggested that other techniques should be used in conjunction with adaptive automation to help minimize the onset of hazardous states of awareness (HSA) and keep the operator 'in-the-loop.' The paper reports on such a technique that uses psychophysiological self-regulation to modulate the level of task engagement. Eighteen participants were assigned to three groups (self-regulation, false feedback, and control) and performed a compensatory tracking task that was cycled between three levels of task difficulty on the basis of the electroencephalogram (EEG) record. Those participants who had received self-regulation training performed significantly better and reported lower NASA-TLX scores than participants in the false feedback and control groups. Furthermore, the false feedback and control groups had significantly more task allocations resulting in return-to-manual performance decrements and higher EEG difference scores. Theoretical and practical implications of these results for adaptive automation are discussed.
Full Text Available Domestic cows (Bos taurus are consumed worldwide as beef and veal, kept as dairy product producers, employed as draft animals in labor, and are used for a long list of other products, including leather and manure. But despite global reliance on cows for thousands of years, most people’s perception of them is as plodding herd animals with little individual personality and very simple social relationships or preferences. Yet, a review of the scientific literature on cow behavior points to more complex cognitive, emotional and social characteristics. Moreover, when cow behavior is addressed, it is almost entirely done within the framework of and applied to their use as food commodities. Therefore, there is relatively little attention to the study of cow intelligence, personality and sociality at a basic comparative level. In this review, we examine the current state of scientific knowledge about cows within an objective comparative framework, describing their cognitive, emotional, and social characteristics. Our aim is to provide a more veridical and objective current summary of cow psychology on its own terms and in ways which will facilitate better-informed comparisons with other animals. Moreover, an understanding of the capabilities and characteristics of domestic cows will, it is hoped, advance our understanding of who they are as individuals.
Cincović, M.R.; Belić, Branislava; Radojičić, Biljana; Hristov, S.; Đoković, R.
The aim of this study was to examine the characteristics of the metabolic profile and complete blood count in cows in the periparturient period on the basis of the intensity of lipolysis and ketogenesis (concentration of non esterified fatty acid - NEFA and betahydroxybutyrate - BHB). Based on median values of NEFA and BHB cows were divided into 3 groups: cows physiologically burdened with catabolism (NEFA and BHB levels above the median one week after part...
Full Text Available One of the most powerful factors of environment that has impact on human body, is low temperature. The degree of its influence depends on the area, containment of the cooling surface of the body, the duration and power of exposure, as well as a number of related factors (humidity of atmospheric air, speed of movement. The ability of thermoregulation in humans provides a constant temperature of the body within certain limits of temperature fluctuations of the environment. These fluctuations are not the same for different people. An important role is played by the individual endurance to cold at the same ambient temperature. The ability to maintain homeostatic temperature balance in the system “organism-environment” is one of the most important features of a living system. The article considers the specifics of adaptation of students from warm countries to the climatic conditions of the Volgograd region. The authors describe the climatic characteristics of the Volgograd region. The weather in winter is characterized by the cyclones, which cause heavy snowfalls, blizzards, strong winds up to 13-18 m/s, temperature decrease (to – 28°С.The article presents the results of the evaluation of the average temperature of the body, the parameters of physical development, data cold test. Increasing the average body temperature after the winter period shows the developing process of adapting the body of international students. At the same time the negative reaction to the cold test was revealed with greater manifestations of students from Turkmenistan. It indicates the presence of adverse reactions and the formation of the difficulties of adaptation processes.
Andrew T. Ludlow
Full Text Available Aging is associated with a tissue degeneration phenotype marked by a loss of tissue regenerative capacity. Regenerative capacity is dictated by environmental and genetic factors that govern the balance between damage and repair. The age-associated changes in the ability of tissues to replace lost or damaged cells is partly the cause of many age-related diseases such as Alzheimer's disease, cardiovascular disease, type II diabetes, and sarcopenia. A well-established marker of the aging process is the length of the protective cap at the ends of chromosomes, called telomeres. Telomeres shorten with each cell division and with increasing chronological age and short telomeres have been associated with a range of age-related diseases. Several studies have shown that chronic exposure to exercise (i.e., exercise training is associated with telomere length maintenance; however, recent evidence points out several controversial issues concerning tissue-specific telomere length responses. The goals of the review are to familiarize the reader with the current telomere dogma, review the literature exploring the interactions of exercise with telomere phenotypes, discuss the mechanistic research relating telomere dynamics to exercise stimuli, and finally propose future directions for work related to telomeres and physiological stress.
Wall, Christopher E; Cozza, Steven; Riquelme, Cecilia A; McCombie, W Richard; Heimiller, Joseph K; Marr, Thomas G; Leinwand, Leslie A
The infrequently feeding Burmese python (Python molurus) experiences significant and rapid postprandial cardiac hypertrophy followed by regression as digestion is completed. To begin to explore the molecular mechanisms of this response, we have sequenced and assembled the fasted and postfed Burmese python heart transcriptomes with Illumina technology using the chicken (Gallus gallus) genome as a reference. In addition, we have used RNA-seq analysis to identify differences in the expression of biological processes and signaling pathways between fasted, 1 day postfed (DPF), and 3 DPF hearts. Out of a combined transcriptome of ∼2,800 mRNAs, 464 genes were differentially expressed. Genes showing differential expression at 1 DPF compared with fasted were enriched for biological processes involved in metabolism and energetics, while genes showing differential expression at 3 DPF compared with fasted were enriched for processes involved in biogenesis, structural remodeling, and organization. Moreover, we present evidence for the activation of physiological and not pathological signaling pathways in this rapid, novel model of cardiac growth in pythons. Together, our data provide the first comprehensive gene expression profile for a reptile heart.
Shih, Kai-Ming; Chang, Chung-Te; Chung, Jeng-Der; Chiang, Yu-Chung; Hwang, Shih-Ying
Double digest restriction site-associated DNA sequencing (ddRADseq) is a tool for delivering genome-wide single nucleotide polymorphism (SNP) markers for non-model organisms useful in resolving fine-scale population structure and detecting signatures of selection. This study performs population genetic analysis, based on ddRADseq data, of a coniferous species, Keteleeria davidiana var. formosana , disjunctly distributed in northern and southern Taiwan, for investigation of population adaptive divergence in response to environmental heterogeneity. A total of 13,914 SNPs were detected and used to assess genetic diversity, F ST outlier detection, population genetic structure, and individual assignments of five populations (62 individuals) of K. davidiana var. formosana . Principal component analysis (PCA), individual assignments, and the neighbor-joining tree were successful in differentiating individuals between northern and southern populations of K. davidiana var. formosana , but apparent gene flow between the southern DW30 population and northern populations was also revealed. Fifteen of 23 highly differentiated SNPs identified were found to be strongly associated with environmental variables, suggesting isolation-by-environment (IBE). However, multiple matrix regression with randomization analysis revealed strong IBE as well as significant isolation-by-distance. Environmental impacts on divergence were found between populations of the North and South regions and also between the two southern neighboring populations. BLASTN annotation of the sequences flanking outlier SNPs gave significant hits for three of 23 markers that might have biological relevance to mitochondrial homeostasis involved in the survival of locally adapted lineages. Species delimitation between K. davidiana var. formosana and its ancestor, K. davidiana , was also examined (72 individuals). This study has produced highly informative population genomic data for the understanding of population
Full Text Available Double digest restriction site-associated DNA sequencing (ddRADseq is a tool for delivering genome-wide single nucleotide polymorphism (SNP markers for non-model organisms useful in resolving fine-scale population structure and detecting signatures of selection. This study performs population genetic analysis, based on ddRADseq data, of a coniferous species, Keteleeria davidiana var. formosana, disjunctly distributed in northern and southern Taiwan, for investigation of population adaptive divergence in response to environmental heterogeneity. A total of 13,914 SNPs were detected and used to assess genetic diversity, FST outlier detection, population genetic structure, and individual assignments of five populations (62 individuals of K. davidiana var. formosana. Principal component analysis (PCA, individual assignments, and the neighbor-joining tree were successful in differentiating individuals between northern and southern populations of K. davidiana var. formosana, but apparent gene flow between the southern DW30 population and northern populations was also revealed. Fifteen of 23 highly differentiated SNPs identified were found to be strongly associated with environmental variables, suggesting isolation-by-environment (IBE. However, multiple matrix regression with randomization analysis revealed strong IBE as well as significant isolation-by-distance. Environmental impacts on divergence were found between populations of the North and South regions and also between the two southern neighboring populations. BLASTN annotation of the sequences flanking outlier SNPs gave significant hits for three of 23 markers that might have biological relevance to mitochondrial homeostasis involved in the survival of locally adapted lineages. Species delimitation between K. davidiana var. formosana and its ancestor, K. davidiana, was also examined (72 individuals. This study has produced highly informative population genomic data for the understanding of
Full Text Available The aim of the study was technological and economical analysis of free range cow breeding. The case study analyzed two different systems of holstein-friesian dairy cows breeding. The model total costs enterprise budget was developed for evaluation of economic feasibility of loose housing dairy cows in comparison with tied cow breeding system. Computer supported calculation enabled estimation of the most important economical parameters (net return, total cost, and coefficient of economics. Results obtained show that (at observed input parameters loose housing system is economically feasible, if there is a minimum of 41 dairy cows with an average milk production of 8610 kg per cow. It was also established that cows need approximately 6 months to fully adapt to the loose housing system.
Moran, Clinton J; Gerry, Shannon P; O'Neill, Matthew W; Rzucidlo, Caroline L; Gibb, Alice C
Morphological streamlining is often associated with physiological advantages for steady swimming in fishes. Though most commonly studied in pelagic fishes, streamlining also occurs in fishes that occupy high-flow environments. Before the installation of dams and water diversions, bonytail (Cyprinidae, Gila elegans ), a fish endemic to the Colorado River (USA), regularly experienced massive, seasonal flooding events. Individuals of G. elegans display morphological characteristics that may facilitate swimming in high-flow conditions, including a narrow caudal peduncle and a high aspect ratio caudal fin. We tested the hypothesis that these features improve sustained swimming performance in bonytail by comparing locomotor performance in G. elegans with that of the closely related roundtail chub ( Gila robusta ) and two non-native species, rainbow trout ( Oncorhynchus mykiss ) and smallmouth bass ( Micropterus dolomieu ), using a Brett-style respirometer and locomotor step-tests. Gila elegans had the lowest estimated drag coefficient and the highest sustained swimming speeds relative to the other three species. There were no detectible differences in locomotor energetics during steady swimming among the four species. When challenged by high-velocity water flows, the second native species examined in this study, G. robusta , exploited the boundary effects in the flow tank by pitching forward and bracing the pelvic and pectoral fins against the acrylic tank bottom to 'hold station'. Because G. robusta can station hold to prevent being swept downstream during high flows and G. elegans can maintain swimming speeds greater than those of smallmouth bass and rainbow trout with comparable metabolic costs, we suggest that management agencies could use artificial flooding events to wash non-native competitors downstream and out of the Colorado River habitat. © 2018. Published by The Company of Biologists Ltd.
Palma, Margarida; Guerreiro, Joana F; Sá-Correia, Isabel
Acetic acid is an important microbial growth inhibitor in the food industry; it is used as a preservative in foods and beverages and is produced during normal yeast metabolism in biotechnological processes. Acetic acid is also a major inhibitory compound present in lignocellulosic hydrolysates affecting the use of this promising carbon source for sustainable bioprocesses. Although the molecular mechanisms underlying Saccharomyces cerevisiae response and adaptation to acetic acid have been studied for years, only recently they have been examined in more detail in Zygosaccharomyces bailii . However, due to its remarkable tolerance to acetic acid and other weak acids this yeast species is a major threat in the spoilage of acidic foods and beverages and considered as an interesting alternative cell factory in Biotechnology. This review paper emphasizes genome-wide strategies that are providing global insights into the molecular targets, signaling pathways and mechanisms behind S. cerevisiae and Z. bailii tolerance to acetic acid, and extends this information to other weak acids whenever relevant. Such comprehensive perspective and the knowledge gathered in these two yeast species allowed the identification of candidate molecular targets, either for the design of effective strategies to overcome yeast spoilage in acidic foods and beverages, or for the rational genome engineering to construct more robust industrial strains. Examples of successful applications are provided.
Guernec, Anthony; Robichaud-Rincon, Philippe; Saucier, Linda
Bacteria on meat are subjected to specific living conditions that differ drastically from typical laboratory procedures in synthetic media. This study was undertaken to determine the behavior of bacteria when transferred from a rich-liquid medium to solid matrices, as is the case during microbial process validation. Escherichia coli cultured in Brain-Heart Infusion (BHI) broth to different growth phases were inoculated in ground beef (GB) and stored at 5°C for 12 days or spread onto BHI agar and cooked meat medium (CMM), and incubated at 37°C for several hours. We monitored cell densities and the expression of σ factors and genes under their control over time. The initial growth phase of the inoculum influenced growth resumption after transfer onto BHI agar and CMM. Whatever the solid matrix, bacteria adapted to their new environment and did not perceive stress immediately after inoculation. During this period, the σ(E) and σ(H) regulons were not activated and rpoD mRNA levels adjusted quickly. The rpoS and gadA mRNA levels did not increase after inoculation on solid surfaces and displayed normal growth-dependent modifications. After transfer onto GB, dnaK and groEL gene expression was affected more by the low temperature than by the composition of a meat environment. Copyright © 2012 Elsevier Ltd. All rights reserved.
Luciane Silva Martello
Full Text Available Este trabalho foi realizado, no verão de 2002, com o objetivo de avaliar a influência de alguns recursos de climatização na produção de leite e na termorregulação dos animais. Foram utilizadas 10 primíparas e 17 multíparas em lactação, distribuídas em instalação controle (ICO, instalação com nebulizador associado a ventiladores (ICL e instalação com tela de sombreamento (IT. A produção de leite e o consumo individual foram medidos diariamente para cada animal. A temperatura retal foi medida três vezes ao dia, em três animais de cada instalação; a temperatura de superfície da pele, três vezes ao dia em todos os animais; e a freqüência respiratória, duas vezes ao dia em todos os animais. Os dados climáticos de cada instalação foram registrados e posteriormente calculados os índices de temperatura e umidade (ITU e de globo negro e umidade (ITGU. Foram selecionados e analisados 26 dias com entalpia elevada. O ITU entre 75 e 76, apesar de considerado estressante por diversas fontes da literatura, não foi associado à condição de estresse pelos animais. As multíparas da instalação climatizada apresentaram freqüência respiratória e temperatura de pele significativamente menor em relação às multíparas das demais instalações. As primíparas apresentaram freqüências respiratórias e temperaturas retais mais altas que as multíparas, nos horários mais quentes do dia. A maior produção de leite das multíparas foi observada no tratamento com tela.This work was carried out during the summer of 2002 to evaluate the influence of some cooling systems on the milk yield and animal thermoregulation. Ten heifers and seventeen milking cows were assigned to the control housing (ICO, mist & fan housing (ICL and shade cloth (80% (IT. The milk yield and the individual intake were daily measured for each animal. The rectal temperature was measured three times a day with three animals from each treatment. The skin
Melissa J Caimano
Full Text Available Leptospirosis, an emerging zoonotic disease with worldwide distribution, is caused by spirochetes belonging to the genus Leptospira. More than 500,000 cases of severe leptospirosis are reported annually, with >10% of these being fatal. Leptospires can survive for weeks in suitably moist conditions before encountering a new host. Reservoir hosts, typically rodents, exhibit little to no signs of disease but shed large numbers of organisms in their urine. Transmission occurs when mucosal surfaces or abraded skin come into contact with infected urine or urine-contaminated water or soil. In humans, leptospires can cause a variety of clinical manifestations, ranging from asymptomatic or mild fever to severe icteric (Weil's disease and pulmonary haemorrhage. Currently, little is known about how Leptospira persist within a reservoir host. Prior in vitro studies have suggested that leptospires alter their transcriptomic and proteomic profiles in response to environmental signals encountered during mammalian infection. However, no study has examined gene expression by leptospires within a mammalian host-adapted state. To obtain a more faithful representation of how leptospires respond to host-derived signals, we used RNA-Seq to compare the transcriptome of L. interrogans cultivated within dialysis membrane chambers (DMCs implanted into the peritoneal cavities of rats with that of organisms grown in vitro. In addition to determining the relative expression levels of "core" housekeeping genes under both growth conditions, we identified 166 genes that are differentially-expressed by L. interrogans in vivo. Our analyses highlight physiological aspects of host adaptation by leptospires relating to heme uptake and utilization. We also identified 11 novel non-coding transcripts that are candidate small regulatory RNAs. The DMC model provides a facile system for studying the transcriptional and antigenic changes associated with mammalian host-adaptation
Soundararajan, Prabhakaran; Manivannan, Abinaya; Ko, Chung Ho; Muneer, Sowbiya; Jeong, Byoung Ryong
understanding on potential mechanism(s) adapted by rose due to the exogenous Si supplementation during the salinity stress.
Wu, Guangxi; Zhao, He; Li, Chenhao; Rajapakse, Menaka Priyadarsani; Wong, Wing Cheong; Xu, Jun; Saunders, Charles W; Reeder, Nancy L; Reilman, Raymond A; Scheynius, Annika; Sun, Sheng; Billmyre, Blake Robert; Li, Wenjun; Averette, Anna Floyd; Mieczkowski, Piotr; Heitman, Joseph; Theelen, Bart; Schröder, Markus S; De Sessions, Paola Florez; Butler, Geraldine; Maurer-Stroh, Sebastian; Boekhout, Teun; Nagarajan, Niranjan; Dawson, Thomas L
Malassezia is a unique lipophilic genus in class Malasseziomycetes in Ustilaginomycotina, (Basidiomycota, fungi) that otherwise consists almost exclusively of plant pathogens. Malassezia are typically isolated from warm-blooded animals, are dominant members of the human skin mycobiome and are associated with common skin disorders. To characterize the genetic basis of the unique phenotypes of Malassezia spp., we sequenced the genomes of all 14 accepted species and used comparative genomics against a broad panel of fungal genomes to comprehensively identify distinct features that define the Malassezia gene repertoire: gene gain and loss; selection signatures; and lineage-specific gene family expansions. Our analysis revealed key gene gain events (64) with a single gene conserved across all Malassezia but absent in all other sequenced Basidiomycota. These likely horizontally transferred genes provide intriguing gain-of-function events and prime candidates to explain the emergence of Malassezia. A larger set of genes (741) were lost, with enrichment for glycosyl hydrolases and carbohydrate metabolism, concordant with adaptation to skin's carbohydrate-deficient environment. Gene family analysis revealed extensive turnover and underlined the importance of secretory lipases, phospholipases, aspartyl proteases, and other peptidases. Combining genomic analysis with a re-evaluation of culture characteristics, we establish the likely lipid-dependence of all Malassezia. Our phylogenetic analysis sheds new light on the relationship between Malassezia and other members of Ustilaginomycotina, as well as phylogenetic lineages within the genus. Overall, our study provides a unique genomic resource for understanding Malassezia niche-specificity and potential virulence, as well as their abundance and distribution in the environment and on human skin.
Full Text Available Malassezia is a unique lipophilic genus in class Malasseziomycetes in Ustilaginomycotina, (Basidiomycota, fungi that otherwise consists almost exclusively of plant pathogens. Malassezia are typically isolated from warm-blooded animals, are dominant members of the human skin mycobiome and are associated with common skin disorders. To characterize the genetic basis of the unique phenotypes of Malassezia spp., we sequenced the genomes of all 14 accepted species and used comparative genomics against a broad panel of fungal genomes to comprehensively identify distinct features that define the Malassezia gene repertoire: gene gain and loss; selection signatures; and lineage-specific gene family expansions. Our analysis revealed key gene gain events (64 with a single gene conserved across all Malassezia but absent in all other sequenced Basidiomycota. These likely horizontally transferred genes provide intriguing gain-of-function events and prime candidates to explain the emergence of Malassezia. A larger set of genes (741 were lost, with enrichment for glycosyl hydrolases and carbohydrate metabolism, concordant with adaptation to skin's carbohydrate-deficient environment. Gene family analysis revealed extensive turnover and underlined the importance of secretory lipases, phospholipases, aspartyl proteases, and other peptidases. Combining genomic analysis with a re-evaluation of culture characteristics, we establish the likely lipid-dependence of all Malassezia. Our phylogenetic analysis sheds new light on the relationship between Malassezia and other members of Ustilaginomycotina, as well as phylogenetic lineages within the genus. Overall, our study provides a unique genomic resource for understanding Malassezia niche-specificity and potential virulence, as well as their abundance and distribution in the environment and on human skin.
Full Text Available This paper describes the genome-based analysis of Tepidanaerobacter acetatoxydans strain Re1, a syntrophic acetate-oxidising bacterium (SAOB. Principal issues such as environmental adaptations, metabolic capacities, and energy conserving systems have been investigated and the potential consequences for syntrophic acetate oxidation discussed. Briefly, in pure culture, T. acetatoxydans grows with different organic compounds and produces acetate as the main product. In a syntrophic consortium with a hydrogenotrophic methanogen, it can also reverse its metabolism and instead convert acetate to formate/H2 and CO2. It can only proceed if the product formed is continuously removed. This process generates a very small amount of energy that is scarcely enough for growth, which makes this particular syntrophy of special interest. As a crucial member of the biogas-producing community in ammonium-rich engineered AD processes, genomic features conferring ammonium resistance, bacterial defense, oxygen and temperature tolerance were found, as well as attributes related to biofilm formation and flocculation. It is likely that T. acetatoxydans can form an electrochemical gradient by putative electron-bifurcating Rnf complex and [Fe-Fe] hydrogenases, as observed in other acetogens. However, genomic deficiencies related to acetogenic metabolism and anaerobic respiration were discovered, such as the lack of formate dehydrogenase and F1F0 ATP synthase. This has potential consequences for the metabolic pathways used under SAO and non-SAO conditions. The two complete sets of bacteriophage genomes, which were found to be encoded in the genome, are also worthy of mention.
Nalcakan Gulbin Rudarli
Full Text Available The purpose of this study was to compare the effects of sprint interval training (SIT and continuous endurance training (CET on selected anthropometric, aerobic, and anaerobic performance indices as well as the blood lipid profile, inflammatory and muscle damage markers in healthy young males. Fifteen recreationally active male volunteers (age: 21.7 ±2.2 years, body mass: 83.0 ±8.0 kg, body height: 1.82 ±0.05 m were divided into two groups according to their initial VO2max levels. Training programs were conducted 3 times per week for 7 weeks. The SIT program consisted of 4-6 Wingate anaerobic sprints with a 4.5 min recovery, while CET consisted of 30-50 min cycling at 60% VO2max. Biochemical, anthropometric and fitness assessments were performed both pre and post-intervention. Significant improvements in VO2max, anaerobic power and capacity, and VO2 utilization during the submaximal workout and significant decreases in body fat and in waist circumference after the intervention occurred in both SIT and CET groups. Significantly greater gross efficiency was measured in the CET group. No differences in the lipid profile or serum levels of inflammatory, myocardial and skeletal muscle damage markers were observed after the training period. The study results agree with the effectiveness of a 30 s all-out training program with a reduced time commitment for anthropometric, aerobic and anaerobic adaptation and eliminate doubts about its safety as a model.
Mad Cow Disease, or bovine spongiform encephalopathy (BSE) is one of ... humoral immunity is developed against such infections. ... Most infecti ve agents, ranging from the more complex protozoans to bacteri(! and viruses, contain nucleic.
C. E. F. Clark
Full Text Available Pasture-based automatic milking systems (AMS require cow traffic to enable cows to be milked. The interval between milkings can be manipulated by strategically allocating pasture. The current experiment investigated the effect of replacing an allocation of grazed pasture with grazed soybean (Glycine max with the hypothesis that incorporating soybean would increase voluntary cow traffic and milk production. One hundred and eighty mixed age, primiparous and multiparous Holstein-Friesian/Illawarra cows were randomly assigned to two treatment groups (n = 90/group with a 2×2 Latin square design. Each group was either offered treatments of kikuyu grass (Pennisetum clandestinum Hoach ex Chiov. pasture (pasture or soybean from 0900 h to 1500 h during the experimental period which consisted of 2 periods of 3 days following 5 days of training and adaptation in each period with groups crossing over treatments after the first period. The number of cows trafficking to each treatment was similar together with milk yield (mean ≈18 L/cow/d in this experiment. For the cows that arrived at soybean or pasture there were significant differences in their behaviour and consequently the number of cows exiting each treatment paddock. There was greater cow traffic (more cows and sooner exiting pasture allocations. Cows that arrived at soybean stayed on the allocation for 25% more time and ate more forage (8.5 kg/cow/d/allocation relative to pasture (4.7 kg/cow/d/allocation. Pasture cows predominantly replaced eating time with rumination. These findings suggest that replacing pasture with alternative grazeable forages provides no additional incentive to increase voluntary cow traffic to an allocation of feed in AMS. This work highlights the opportunity to increase forage intakes in AMS through the incorporation of alternative forages.
Effects of ruminal ammonia and butyrate concentrations on reticuloruminal epithelial blood flow and volatile fatty acid absorption kinetics under washed reticulorumen conditions in lactating dairy cows
Storm, Adam Christian; Hanigan, M.D.; Kristensen, Niels Bastian
and mesenteric, right ruminal, and hepatic portal veins. The experiment was designed with 2 groups of cows: 4 cows adapted to high crude protein (CP) and 4 to low CP. All cows were subjected to 3 buffers: butyric, ammonia, and control in a randomized replicated 3 × 3 incomplete Latin square design. The buffers...
María Luisa eDurán-Pastén
Full Text Available Pituitary gonadotrophs are a small fraction of the anterior pituitary population, yet they synthesize gonadotropins: luteinizing (LH and follicle stimulating (FSH, essential for gametogenesis and steroidogenesis. LH is secreted via a regulated pathway while FSH release is mostly constitutive and controlled by synthesis. Although gonadotrophs fire action potentials spontaneously, the intracellular Ca2+ rises produced do not influence secretion, which is mainly driven by Gonadotropin Releasing Hormone (GnRH, a decapeptide synthesized in the hypothalamus and released in a pulsatile manner into the hypophyseal portal circulation. GnRH binding to G protein coupled receptors triggers Ca2+ mobilization from InsP3-sensitive intracellular pools, generating the global Ca2+ elevations necessary for secretion. Ca2+ signaling responses to increasing [GnRH] vary in stereotyped fashion from subthreshold to baseline spiking (oscillatory, to biphasic (spike-oscillatory or spike-plateau. This progression varies somewhat in gonadotrophs from different species and biological preparations. Both baseline spiking and biphasic GnRH-induced Ca2+ signals control LH/FSH synthesis and exocytosis. Estradiol and testosterone regulate gonadotropin secretion through feedback mechanisms, while FSH synthesis and release are influenced by activin, inhibin and follistatin. Adaptation to physiological events like the estrous cycle, involves changes in GnRH sensitivity and LH/FSH synthesis: in proestrus, estradiol feedback regulation abruptly changes from negative to positive, causing the pre-ovulatory LH surge. Similarly, when testosterone levels drop after orquiectomy the lack of negative feedback on pituitary and hypothalamus boosts both GnRH and LH secretion, gonadotrophs GnRH sensitivity increases and Ca2+ signaling patterns change. In addition, gonadotrophs proliferate and grow. These plastic changes denote a more vigorous functional adaptation in response to an extraordinary
Full Text Available A striking characteristic of endemic Antarctic brown algae is their broad vertical distribution. This feature is largely determined by the shade adaptation in order to cope with the seasonal variation in light availability. However, during spring-summer months, when light penetrates deep in the water column these organisms have to withstand high levels of solar radiation, including UV. In the present study we examine the light use characteristics in parallel to a potential for UV tolerance (measured as content of phenolic compounds, antioxidant activity and maximum quantum yield of fluorescence in conspecific populations of four Antarctic brown algae (Ascoseira mirabilis, Desmarestia menziesii, D. anceps and Himantothallus grandifolius distributed over a depth gradient between 5 and 30 m. The main results indicated that a photosynthetic efficiency was uniform along the depth gradient in all the studied species, and b short-term (6 h exposure to UV radiation revealed a high tolerance measured as chlorophyll fluorescence, phlorotannin content and antioxidant capacity. Multivariate analysis of similarity indicated that light requirements for photosynthesis, soluble phlorotannins and antioxidant capacity are the variables determining the responses along the depth gradient in all the studied species. The suite of physiological responses of algae with a shallower distribution (A. mirabilis and D. menziesii differed from those with deeper vertical range (D. anceps and H. grandifolius. These patterns are consistent with the underwater light penetration that defines two zones: 0-15 m, with influence of UV radiation (1% of UV-B and UV-A at 9 m and 15 m respectively and a zone below 15 m marked by PAR incidence (1% up to 30 m. These results support the prediction that algae show a UV stress tolerance capacity along a broad depth range according to their marked shade adaptation. The high contents of phlorotannins and antioxidant potential appear to be
Rutherfurd, S.M.; Darragh, A.J.; Hendriks, W.H.; Prosser, C.G.; Lowry, D.
Goat milk and cow milk are commonly used in infant formula preparations and, as such, understanding the nutritional characteristics of infant formulas made from these milks is important. In this study, a goat milk infant formula was compared with an adapted (whey-enhanced) cow milk infant formula
Cow's milk allergy is more common in children than in adults. CaSSim ... adverse reactions to cow's milk protein such as lactose intolerance. .... possible hormonal effects on the reproductive ... formula in humans – such studies are much.
den Hoed, M; Smeets, A J P G; Veldhorst, M A B; Nieuwenhuizen, A G; Bouwman, F G; Heidema, A G; Mariman, E C M; Westerterp-Plantenga, M S; Westerterp, K R
The postprandial responses in (an)orexigenic hormones and feelings of hunger are characterized by large inter-individual differences. Food intake regulation was shown earlier to be partly under genetic control. This study aimed to determine whether the postprandial responses in (an)orexigenic hormones and parameters of food intake regulation are associated with single nucleotide polymorphisms (SNPs) in genes encoding for satiety hormones and their receptors. Peptide YY (PYY), glucagon-like peptide 1 and ghrelin levels, as well as feelings of hunger and satiety, were determined pre- and postprandially in 62 women and 41 men (age 31+/-14 years; body mass index 25.0+/-3.1 kg/m(2)). Dietary restraint, disinhibition and perceived hunger were determined using the three-factor eating questionnaire. SNPs were determined in the GHRL, GHSR, LEP, LEPR, PYY, NPY, NPY2R and CART genes. The postprandial response in plasma ghrelin levels was associated with SNPs in PYY (215G>C, PG and 688A>G, PGHRL (-501A>C, PA, PG and 585T>C, PA, PA and 204T>C, P<0.05). Part of the inter-individual variability in postprandial responses in (an)orexigenic hormones can be explained by genetic variation. These postprandial responses represent either long-term physiological adaptations to facilitate homeostasis or reinforce direct genetic effects.
Amrit, Francis Raj Gandhi; Steenkiste, Elizabeth Marie; Ratnappan, Ramesh; Chen, Shaw-Wen; McClendon, T. Brooke; Kostka, Dennis; Yanowitz, Judith; Olsen, Carissa Perez; Ghazi, Arjumand
Elimination of the proliferating germline extends lifespan in C. elegans. This phenomenon provides a unique platform to understand how complex metazoans retain metabolic homeostasis when challenged with major physiological perturbations. Here, we demonstrate that two conserved transcription regulators essential for the longevity of germline-less adults, DAF-16/FOXO3A and TCER-1/TCERG1, concurrently enhance the expression of multiple genes involved in lipid synthesis and breakdown, and that both gene classes promote longevity. Lipidomic analyses revealed that key lipogenic processes, including de novo fatty acid synthesis, triglyceride production, desaturation and elongation, are augmented upon germline removal. Our data suggest that lipid anabolic and catabolic pathways are coordinately augmented in response to germline loss, and this metabolic shift helps preserve lipid homeostasis. DAF-16 and TCER-1 also perform essential inhibitory functions in germline-ablated animals. TCER-1 inhibits the somatic gene-expression program that facilitates reproduction and represses anti-longevity genes, whereas DAF-16 impedes ribosome biogenesis. Additionally, we discovered that TCER-1 is critical for optimal fertility in normal adults, suggesting that the protein acts as a switch supporting reproductive fitness or longevity depending on the presence or absence of the germline. Collectively, our data offer insights into how organisms adapt to changes in reproductive status, by utilizing the activating and repressive functions of transcription factors and coordinating fat production and degradation. PMID:26862916
Bionaz, Massimo; Loor, Juan J.
High-throughput ‘omics’ data analysis via bioinformatics is one key component of the systems biology approach. The systems approach is particularly well-suited for the study of the interactions between nutrition and physiological state with tissue metabolism and functions during key life stages of organisms such as the transition from pregnancy to lactation in mammals, ie, the peripartal period. In modern dairy cows with an unprecedented genetic potential for milk synthesis, the nature of the physiologic and metabolic adaptations during the peripartal period is multifaceted and involves key tissues such as liver, adipose, and mammary. In order to understand such adaptation, we have reviewed several works performed in our and other labs. In addition, we have used a novel bioinformatics approach, Dynamic Impact Approach (DIA), in combination with partly previously published data to help interpret longitudinal biological adaptations of bovine liver, adipose, and mammary tissue to lactation using transcriptomics datasets. Use of DIA with transcriptomic data from those tissues during normal physiological adaptations and in animals fed different levels of energy prepartum allowed visualization and integration of most-impacted metabolic pathways around the time of parturition. The DIA is a suitable tool for applying the integrative systems biology approach. The ultimate goal is to visualize the complexity of the systems at study and uncover key molecular players involved in the tissue’s adaptations to physiological state or nutrition. PMID:22807626
Vargas-Bello-Pérez, Einar; Íñiguez-González, Gonzalo; Cancino-Padilla, Nathaly; Loor, Juan J; Garnsworthy, Philip C
The aim of this study was to elucidate the effect of dietary supplementation of soybean oil (SO) and hydrogenated palm oil (HPO) on the transport of fatty acids (FA) within plasma lipoproteins in lactating and non-lactating cows. Three lactating and three non-lactating Holstein cows were used in two different 3 × 3 Latin square experiments that included three periods of 21 d. Dietary treatments for lactating cows consisted of a basal diet (control; no fat supplement) and fat-supplemented diets containing SO (500 g/d per cow) or HPO (500 g/d per cow). For non-lactating cows, dietary treatments consisted of a basal diet (control; no fat supplement) and fat-supplemented diets containing SO (170 g/d per cow) or HPO (170 g/d per cow). Compared with the control and SO diet, HPO addition increased (p lipoprotein (HDL). Total saturated FA were increased (p lipoprotein (VLDL). In non-lactating cows, the concentration of C18:0 was increased (p lipoprotein. Overall, it was found that distribution and transport of FA within the bovine plasma lipoproteins may be influenced by chain length and degree of unsaturation of dietary lipids. Also, the distribution of individual FA isomers such as C18:1trans-11 and C18:2cis-9,trans-11 may vary depending on the physiological state of the cow (lactating or non-lactating), and are increased in plasma (lactating cows) and the HDL (non-lactating cows) when cows are fed SO.
Heublein, C; Dohme-Meier, F; Südekum, K-H; Bruckmaier, R M; Thanner, S; Schori, F
As ruminants are able to digest fibre efficiently and assuming that competition for feed v. food use would intensify in the future, cereals and other field crops should primarily be destined to cover the dietary needs of humans and monogastric animals such as poultry and pigs. Farming systems with a reduced or absent concentrate supplementation, as postulated by organic agriculture associations, require adapted dairy cows. The aim of this experiment was to examine the impact of concentrate supplementation on milk production, grazing and rumination behaviour, feed intake, physical activity and blood traits with two Holstein-Friesian cow strains and to conclude the consequences for sustainable and organic farming. The experiment was a cross-over study and took place on an organic farm in Switzerland. In all, 12 Swiss Holstein-Friesian (HCH) cows and 12 New Zealand Holstein-Friesian (HNZ) cows, which were paired according to lactation number, days in milk and age for primiparous cows, were used. All cows grazed full time and were supplemented either with 6 kg/day of a commercial, organic cereal-grain mix or received no supplement. After an adaptation period of 21 days, a measurement period of 7 days followed, where milk yield and composition, pasture dry matter intake estimated with the n-alkane double-indicator technique, physical activity based on pedometer measurements, grazing behaviour recorded by automatic jaw movement recorder and blood samples were investigated. Non-supplemented cows had a lower milk yield and supplemented HCH cows produced more milk than supplemented HNZ cows. Grazing time and physical activity were greater for non-supplemented cows. Supplementation had no effect on rumination behaviour, but HNZ cows spent longer ruminating compared with HCH cows. Pasture dry matter intake decreased with the concentrate supplementation. Results of blood analysis did not indicate a strong negative energy balance for either non-supplemented or supplemented cows
Høst, Arne; Halken, Susanne
Since the 1930's the scientific literature on cow's milk protein allergy (CMPA) has accumulated. Over the last decade new diagnostic tools and treatment approaches have been developed. The diagnosis of reproducible adverse reactions to cow's milk proteins (CMP), i.e. CMPA, still has to be confirmed...... by controlled elimination and challenge procedures. Advanced diagnostic testing using epitope and microarray technology may in the future improve the diagnostic accuracy of CMPA by determination of specific IgE against specific allergen components of cow's milk protein. The incidence of CMPA in early childhood...... is approximately 2-3% in developed countries. Symptoms suggestive of CMPA may be encountered in 5-15% of infants emphasizing the importance of controlled elimination/milk challenge procedures. Reproducible clinical reactions to CMP in human milk have been reported in 0.5% of breastfed infants. Most infants...
Katie Persons, Biologist Peter ’Ringens, Biochemist Oliver Heroux, Ph.D., Physiologist Edward Koo, Biologist Martin Steiner, Biologist Louise Janes...and aspects . This manuscript includes about one-fifth of our data on this topic, obtained in the last two years. Of course, my motivation for studying...ARCTIC LEIMINGS Progressive depression of renal function during hypo- thermia generally is presupposed. Data gathered incidentally as part of a
Ion Valeriu Caraba
Full Text Available As a research on the reproductive physiology and endocrinology surrounding the estrous cycle in dairy cattle has been compiled, several estrous synchronization programs have been developed for use with dairy cows. These include several programs that facilitate the mass breeding of all animals at a predetermined time (timed-AI rather than the detection of estrus. We studied on 15 dary cows which were synchronized by Ovsynch and Cosynch programs. The estrus response for cows in Ovsynch protocol was of 63%. Pregnancy per insemination at 60 days was of 25%. Estrus response for cow in Cosynch protocol was of 57%. Pregnancy per insemination at 60 days was of 57%. Synchronization of ovulation using Ovsynch protocols can provide an effective way to manage reproduction in lactating dairy cows by eliminating the need for estrus detection. These are really efficient management programs for TAI of dairy cows that are able to reduce both the labour costs and the extra handling to daily estrus detection and AI.
Full Text Available Purpose: The present study evaluated the effects of regular participation in small-sided team handball training on body composition, osteogenic response, physical performance, and cardiovascular risk factors, as well as well-being and motivation, in young untrained women. Methods: Twenty-eight untrained 20- to 30-year-old women were randomized to a handball training group (HG; n = 14, height 170 ± 5 cm, weight 73 ± 11 kg, VO2peak 37.7 ± 4.1 mL/min/kg that trained 1.7 ± 0.3 times per week over 12 weeks (70 min 4 v 4 handball sessions or an inactive control group (CG; n = 14, 169 ± 5 cm, 71 ± 12 kg, 38.1 ± 3.7 mL/min/kg. Physiological and psychological and motivational training adaptations were assessed pre- and post-intervention by dual-energy X-ray Absorptiometry (DXA scans, blood sampling, physical tests, and questionnaires. Results: The average heart rate (HR over all training sessions was equal to 85% ± 6% HRmax. Between-group intervention effects were observed in favor of HG for muscle mass (2.1%, p = 0.024, proximal femur bone mineral density (0.8%, p = 0.041, Yo-Yo IE1 intermittent endurance test level 1 (IE1 performance (35%, p < 0.001, and incremental treadmill test performance (11.5%, p = 0.003, but not total fat mass (p = 0.176, mean arterial blood pressure (p = 0.328, resting HR (p = 0.219, or blood lipids (p = 0.298–0.854. In CG, no changes were observed in any of the measured physiological variables after the training period. Compared to CG, HG had an increase in intrinsic motivation (p < 0.001 and in the well-being subscale “energy” (p = 0.010. Conclusion: Participation in regular recreational team handball training organized as small-sided games has marked beneficial effects on physical performance, musculoskeletal fitness, well-being, and motivation in untrained young women. Keywords: Bone mineral density (BMD, Intensity
Full Text Available Assessing the metabolic profile based on the concept that the laboratory measurement of certain circulating components is a tool to evaluate metabolic status of dairy cows. Veterinarian also can evaluate the energy input-output relationships by assessing the metabolic profile to prevent and control of negative energy balance, metabolic disorders and nutritional insufficiencies. In the present study, 25 multiparous Holstein dairy cows were divided to 5 equal groups containing early, mid and late lactation, and far-off and close-up dry. Blood samples were collected from all cows through jugular venipuncture and sera were evaluated for glucose, insulin, β-hydroxybutyric acid (BHBA, non-esterified fatty acid (NEFA, cholesterol, triglyceride (TG, high, low and very low density lipoproteins (HDL, LDL and VLDL. Insulin levels in mid lactation and close-up dry cows were significantly higher than other groups (P<0.05 and the lowest insulin concentration was detected in far-off dry group. Serum concentrations of NEFA and BHBA in early and mid-lactation and close-up dry cows were significantly higher than late lactation and far-off dry animals (P<0.05. Baseline levels of cholesterol in mid and late lactation were significantly higher than other groups. The level of LDL in mid lactation cows was higher than others significantly, and its value in far-off dry cows was significantly lower than other group (P<0.05. It may be concluded that the detected changes among different groups induce commonly by negative energy balance, lactogenesis and fetal growth in each state. The presented metabolic profile can be considered as a tool to assess the energy balance in dairy cows at different physiologic states. It can be used to evaluate the metabolic situations of herd and manage the metabolic and production disorders.
Rigsby, C M; Showalter, D N; Herms, D A; Koch, J L; Bonello, P; Cipollini, D
Emerald ash borer, Agrilus planipennis Fairmaire, an Asian wood-boring beetle, has devastated ash (Fraxinus spp.) trees in North American forests and landscapes since its discovery there in 2002. In this study, we collected living larvae from EAB-resistant Manchurian ash (Fraxinus mandschurica), and susceptible white (Fraxinus americana) and green (Fraxinus pennsylvanica) ash hosts, and quantified the activity and production of selected detoxification, digestive, and antioxidant enzymes. We hypothesized that differences in larval physiology could be used to infer resistance mechanisms of ash. We found no differences in cytochrome P450, glutathione-S-transferase, carboxylesterase, sulfotransferase, and tryptic BApNAase activities between larvae feeding on different hosts. Despite this, Manchurian ash-fed larvae produced a single isozyme of low electrophoretic mobility that was not produced in white or green ash-fed larvae. Additionally, larvae feeding on white and green ash produced two serine protease isozymes of high electrophoretic mobility that were not observed in Manchurian ash-fed larvae. We also found lower activity of β-glucosidase and higher activities of monoamine oxidase, ortho-quinone reductase, catalase, superoxide dismutase, and glutathione reductase in Manchurian ash-fed larvae compared to larvae that had fed on susceptible ash. A single isozyme was detected for both catalase and superoxide dismutase in all larval groups. The activities of the quinone-protective and antioxidant enzymes are consistent with the resistance phenotype of the host species, with the highest activities measured in larvae feeding on resistant Manchurian ash. We conclude that larvae feeding on Manchurian ash could be under quinone and oxidative stress, suggesting these may be potential mechanisms of resistance of Manchurian ash to EAB larvae, and that quinone-protective and antioxidant enzymes are important counter-adaptations of larvae for dealing with these resistance
Gartland, P; Schiavo, J; Hall, C E; Foote, R H; Scott, N R
Electrical resistance (ohms) of mucus were analyzed in 20 postpartum Holstein cows by use of a probe inserted into the anterior vagina every other day for 30 days. Composite milk samples were taken on the same day, and progesterone was determined by radioimmunoassay. Cows were observed twice daily for standing estrus and reproductive organs palpated weekly per rectum (rectal palpation). Fifteen cows which were cycling showed increasing progesterone 6 to 7 days after the onset of estrus with values of 8.1 to 10.0 ng progesterone/ml milk on days 10 to 17. Concentrations had declined rapidly 2 days before onset of the next estrus. Progesterone in milk was affected by cow and by day of the cycle. Electrical resistance followed a similar cyclical pattern, but variability was large and only cows differed. The correlation between milk progesterone and mucus resistance was .22. Progesterone concentrations for four cows with follicular cysts fluctuated randomly with a mean of 2.6 ng/ml. Mean resistance of vaginal mucus was 44 omega for both cycling and cystic cows, indicating that a single measurement of electrical resistance every 2nd day was unreliable in distinguishing physiological states. One cow had high progesterone in milk on days 19 to 25 and was diagnosed pregnant by rectal palpation 3 wk later. Cows were not seen in estrus 28% of the time when milk progesterone and rectal palpation indicated they were in the follicular phase of the estrous cycle and were cycling.
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Correa-Calderon, Abelardo; Armstrong, Dennis; Ray, Donald; DeNise, Sue; Enns, Mark; Howison, Christine
. Thirty-seven Holstein and 26 Brown Swiss dairy cows were used to evaluate the effect of two different cooling systems on physiological and hormonal responses during the summer. A control group of cows had access only to shade (C). A second group was cooled with spray and fans (S/F) and the third group was under an evaporative cooling system called Korral Kool (KK). The maximum temperature humidity index during the trial was from 73 to 85. Rectal temperatures and respiration rates of the C group were higher (P cows. Triiodothyronine levels in milk were higher (P cows during summer in hot, dry climates.
Alex Sandro Campos Maia
. These traits were measured in an area 20cm below the dorsal line in the center of the thorax, for the black as for the white haircoat. Hair samplings were performed with adapted pliers. The method of Restricted Maximum Likelihood (REML was used to estimate the variance and covariance components under the Animal model, the program used being the MTDFREML. The results showed that traits of black haircoat were different from that of the white haircoat, when the animals are breed in a tropical environment. Black haircoat was less dense, with shorter and ticker hair fibres in order to ease the heat dissipation, while the white haircoat was denser with longer hairs, which favored protection against direct solar radiation. The selection of predominantly black cows would be a good choice under the strong solar radiation in tropical environment, because the pigmented skin under this type coat. Such a selection could be easily performed considering the high heritability (h²=0.75 of the proportion of black coat, and must consider a less dense haircoat with short and tick hairs to help the dissipation sensible and latent heat.
After recalling the medical reluctance as well as the risks that there are in complete elimination of milk in infants, the author presents several clinical pictures and then a classification of the immunological types: Allergic shock of neonates, digestive and extra-digestive (skin and respiratory airways) symptoms finally the rare chronic gastro-enteritis to cow milk. Non-reaginic food allergies: Acute gastro-enteropathy to cow milk, with villous atrophy and Heiner's syndrome, delayed hypersensitivities are studied, of difficult diagnosis that may cover almost all pathologies. They may be found in the digestive system, respiratory, the kidneys and even in the organs of behaviour. Migraine of food origin must be remembered. Development in regressive rules is a function of the type of allergy and the suddenness of the symptoms. Diagnosis is above all by questioning and confirmation or not by skin and in vitro tests. Certainty can only be shown by tests of elimination and re-introduction. The diet, at the same time of both diagnostic and therapeutic value, is based on the replacement of cow milk by foods that contain the same amount of proteins. It is essential, especially in the very small, to have perfect match of food so as to avoid any risk of a dramatic hypoprotinemia, which may happen if the child does not like the suggested diet, or if the parents cannot buy the substitution products. In such conditions great care must be taken to avoid provoking a crisis. Care must be taken to decide: If the elimination of cow milk is always justified each time. If it is, always check that the substituted protein is properly made, the family may change the diet mistakenly.
This review pulls together the results of over 40 research projects and brings us up to date with the latest in thinking on dairy cow nutrition, incorporates the findings of a wide range of organic trials and draws some clear recommendations on appropriate strategies for forage type and management, supplementary feeding, ration formulation and farming systems. It raises important issues around sustainability versus optimum production and highlights future research priorities.
Heterosis and direct effects for Charolais-sired calf weight and growth, cow weight and weight change, and ratios of cow and calf weights and weight changes across warm season lactation in Romosinuano, Angus, and F1 cows in A
The use of Brahman in cow-calf production in the southeastern U.S. offers some adaptation to the harsh characteristics of endophyte-infected tall fescue. Other breeds, such as the Criollo breed Romosinuano, may provide similar adaptative characteristics. The objectives were to evaluate Romosinuano...
Full Text Available Requirements on increasing economic efficiency of cattle breeding force farmers to use the latest up-to-datetechnology for monitoring and management of farming quality. Regular weighing and data processing can forinstance discover mistakes that can indicate defects, e.g. nutrition deficiencies, incorrect embryonic development,health problems, demanding nursing intervention. The aim of the research was to monitor manifestations of diseasesand metabolic disorders in the course of weight curve based on data from an automated system for weighing the liveweight of dairy cows. There was used in the weighing unit for milking robots Astronaut A3 (Lely company to obtainweight data of individual cows. There were selected dairy cows with the longest period of lactation or already dryingoff, and especially dairy cows with various health problems for study. Limiting values of weight changes wereestablished after assembling a general equation of mass curve. In the sphere of the diseases there was manifestedonly ketosis in the weight curves with a loss of 10.2 kg / day (38% weight loss. The results of the study will beapplied for compiling algorithm that will be implemented in the complete management system of cattle breeding,monitoring the dairy cows every day and highlight possible deviations exceeding of physiological changes in weight.
Vidić Branka M.
Full Text Available Data on L. hardjo infection of dairy cows in the world pint out its important role in the occurrence of health and economic problem. L. interrogans serotype hardjo has been described as the cause of miscarriages, stillbirts, or the birhs of poorly vital calves, agalactia, mastitis, and low fertility in cows. Two L. hardjo genotypes have been identified in cows, namely, hardjopraitno and hardjobovis. Serological investigations have established a drastic increase in this leptospiral infection in cows. L. hardjo has become adapted to cattle as the primary host, so that an infection is maintained in herds and becomes deeply rooted because of the permanent presence of the source of infection. It was believed that sheep were accidental hosts, but the latest research suggest that they are yet another, transitory, host for maintining this leptospira serotype. L. hardjo is also important from the aspect of human health, especially of persons who are professionally exposed to this infection. L. hardjo infection is detected using serological tests and by proving the presence of leptospira. The medicine of choice in the therapy of leptospiral infections is streptomycin (DSM. Therapy using oxytetracyclines for clinical mastitis was also proven effective. Treatment is most successful in the early stage of the disease. A single dose of streptomycin administered in infected herds reduces the duration period of leptospira excretion through urine, thus preventing the spread of infection thorugh contaminated urine. The basic components of the plan to contain leptospira are the following: serological investigations, sanitary-higiene measures, the elimination of animals which excrete leptospira through urine, therapy, vaccination, quarantine.
Weikard, Rosemarie; Demasius, Wiebke; Hadlich, Frieder; Kühn, Christa
Periparturient cows have been found to reveal immunosuppression, frequently associated with increased susceptibility to uterine and mammary infections. To improve understanding of the causes and molecular regulatory mechanisms accounting for this phenomenon around calving, we examined the effect of an antigen challenge on gene expression modulation on cows prior to (BC) or after calving (AC) using whole transcriptome sequencing (RNAseq). The transcriptome analysis of the cows’ blood identified a substantially higher number of loci affected in BC cows (2,235) in response to vaccination compared to AC cows (208) and revealed a divergent transcriptional profile specific for each group. In BC cows, a variety of loci involved in immune defense and cellular signaling processes were transcriptionally activated, whereas protein biosynthesis and posttranslational processes were tremendously impaired in response to vaccination. Furthermore, energy metabolism in the blood cells of BC cows was shifted from oxidative phosphorylation to the glycolytic system. In AC cows, the number and variety of regulated pathways involved in immunomodulation and maintenance of immnunocompetence are considerably lower after vaccination, and upregulation of arginine degradation was suggested as an immunosuppressive mechanism. Elevated transcript levels of erythrocyte-specific genes involved in gas exchange processes were a specific transcriptional signature in AC cows pointing to hematopoiesis activation. The divergent and substantially lower magnitude of transcriptional modulation in response to vaccination in AC cows provides evidence for a suppressed immune capacity of early lactating cows on the molecular level and demonstrates that an efficient immune response of cows is related to their physiological and metabolic status. PMID:26317664
Xu, Chuang; Shu, Shi; Xia, Cheng; Wang, Pengxian; Sun, Yuhang; Xu, Chuchu; Li, Changsheng
Ketosis is an important metabolic disorder in dairy cows during the transition period. The urine proteomics of ketosis has not been investigated using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). The aim is to determine differences between urine proteomic profiles of healthy cows and those with clinical ketosis, and facilitate studies of the underlying physiological and biochemical mechanisms that lead to liver pathology in ketosis. We analyzed the urine samples of 20 cows with clinical ketosis (group 1) and 20 control cows (group 2) using SELDI-TOF-MS. Thirty-nine peptide peaks differed between both groups. Polypeptides corresponding to 26 of these differential peptide peaks were identified using the SWISS-PROT protein database. We found that the peaks of 11 distinct polypeptides from the urine samples of the ketosis group were significantly reduced, compared with those of the control group as based on the Wilcoxon rank sum test. Among these were VGF (non-acronymic) protein, amyloid precursor protein, serum amyloid A (SAA), fibrinogen, C1INH, apolipoprotein C-III, cystatin C, transthyretin, hepcidin, human neutrophil peptides, and osteopontin. These proteins may represent novel biomarkers of the metabolic changes that occur in dairy cows with ketosis. Our results will help to better understand the physiological changes and pathogenesis observed in cows with ketosis. The SELDI-TOF-MS can be used to understand the physiological and biochemical mechanisms of ketosis and identify biomarkers of the disease.
P.A. Counce; Davidi R. Gealy; Shi-Jean Susana Sung
Physiology occurs tn physical space through chemical reactions constrained by anatomy and morphology, yet guided by genetics. Physiology has been called the logic of life. Genes encode structural and fimcdonal proteins. These proteins are subsequently processed to produce enzymes that direct and govern the biomechanical processes involved in the physiology of the...
Madsen, Torben Gosvig; Nielsen, Mette Benedicte Olaf; Andersen, Jens Bech
Reports over the past decade have indicated that normal lactational performance can be achieved in genetically superior and high-producing dairy cows, even when the dry period between 2 lactations is omitted. The hypothesis tested in this experiment was that normal lactogenesis I and metabolic...... function may be achievable in continuously milked high-yielding dairy cows as a result of the genetic selection for lactation performance and hence longevity of mammary epithelial cells. The milk production and mammary nutrient uptake in response to omission of the dry period for cows with an expected peak...... milk yield higher than 45 kg/d were studied in 28 Holstein dairy cows managed without bovine somatotropin. Performance and metabolic parameters were followed in late gestation and in the following early lactation. Fourteen cows were milked continuously throughout late gestation, and another 14 dairy...
Broucek, Jan; Uhrincat, Michal; Mihina, Stefan; Soch, Miloslav; Mrekajova, Andrea; Hanus, Anton
Simple Summary The purpose of this study was to evaluate the influence of moving cows from the barn with stanchion-stall housing to free-stall housing on their behaviour and production. Cows lay down up to ten hours after removing. The cows in their second lactation and open cows tended to lie sooner after removing than cows in their first lactation and pregnant cows. The times of total lying and rumination were increasing from the first day to the tenth day after removing. Cows produced 23.3% less milk at the first day following the transfer than at the last day prior to moving (23.76 ± 7.20 kg vs. 30.97 ± 7.26 kg, p cows achieved maximum production. The difference was found in milk losses due to the shift between cows in first and second lactation. Abstract Transfer of cattle to an unknown barn may result in a reduction in its welfare. Housing and management practices can result in signs of stress that include a long-term suppression of milk efficiency. The purpose of this study was to evaluate the influence of moving cows from the stanchion-stall housing to free-stall housing on their behaviour and production. The Holstein cows were moved into the new facility with free-stall housing from the old barn with stanchion-stall housing. Cows lay down up to ten hours (596.3 ± 282.7 min) after removing. The cows in their second lactation and open cows tended to lie sooner after removing than cows in their first lactation and pregnant cows. The times of total lying and rumination were increasing from the first day to the tenth day after removing (23.76 ± 7.20 kg vs. 30.97 ± 7.26 kg, p Cows produced 23.3% less milk at the first day following the transfer than at the last day prior to moving (p cows on the first and second lactation (p cows’ milk production. However, when the cows are moved to a better environment, they rapidly adapt to the change. PMID:28273810
Cafarchia, C.; Latrofa, M.S.; Figueredo, L.A.; da Silva Machado, M.L.; Ferreiro, L.; Guillot, J.; Boekhout, T.; Otranto, D.
Three lipid-dependent Malassezia isolates (here named 114A, 114B and 114C) recovered from a dog with skin lesions were phenotypically and genotypically characterized. All presented ovoid cells and buds formed on a narrow base. Most of the results from physiological tests were consistent with those
Upper respiratory tract nociceptor stimulation and stress response following acute and repeated Cyfluthrin inhalation in normal and pregnant rats: Physiological rat-specific adaptions can easily be misunderstood as adversities.
This paper reviews the results from past regulatory and mechanistic inhalation studies in rats with the type II pyrethroid Cyfluthrin. Apart from many chemical irritants, Cyfluthrin was shown to be a neuroexcitatory agent without any inherent tissue-destructive or irritant property. Thus, any Cyfluthrin-induced neuroexcitatory afferent sensory stimulus from peripheral nociceptors in the upper respiratory tract is likely to be perceived as a transient stimulus triggering annoyance and/or avoidance by both rats and humans. However, while thermolabile rats respond to such stresses reflexively, homeothermic humans appear to respond psychologically. With this focus in mind, past inhalation studies in rats and human volunteers were reevaluated and assessed to identify common denominators to such neuroexcitatory stimuli upon inhalation exposure. This analysis supports the conclusion that the adaptive physiological response occurring in rats secondary to such chemosensory stimuli requires inhalation exposures above the chemosensory threshold. Rats, a species known to undergo adaptively a hibernation-like physiological state upon environmental stresses, experienced reflexively-induced bradypnea, bradycardia, hypothermia, and changes in acid-base status during inhalation exposure. After cessation of the sensory stimulus, rapid recovery occurred. Physiological data of male and female rats from a 4-week repeated inhalation study (exposure 6-h/day, 5-times/week) were used to select concentration for a 10-day developmental inhalation toxicity study in pregnant rats. Maternal hypothermia and hypoventilation were identified as likely cause of fetal and placental growth retardations because of a maternal adaptation-driven reduced feto-placental transfer of oxygen. In summary, maternal reflex-hypothermia, reduced cardiac output and placental perfusion, and disruption of the gestation-related hyperventilation are believed to be the maternally mediated causes for developmental
Zambrano-Nava, Sunny; Boscán-Ocando, Julio; Nava, Jexenia
In order to describe the normal bacterial flora in vaginas of Criollo Limonero cows, 51 healthy multiparous cows, at least 90-day postpartum, were selected. Duplicated swabs (N = 102) were taken from the vaginal fornix of cows to perform aerobic and anaerobic cultures as well as conventional biochemical tests. Out of 102 swabs, bacterial growth was obtained in 55 (53.9%) while the remaining 47 (46.1%) did not exhibited any bacterial growth. Of the 55 bacterial growths, 23 (41.8%) were aerobic whereas 32 (58.1%) were anaerobic. Likewise, 29 (52.72%) of bacterial growths were pure and 26 (47.27%) were mixed. Under both aerobic and anaerobic conditions, Gram positive bacteria were predominant (81.82% and 73.08%, respectively) over Gram negative bacteria (18.18% and 26.92%, respectively). Isolated bacteria were Arcanobacterium pyogenes (22.92%), Staphylococcus aureus (15.63%), Staphylococcus coagulase negative (17.71%), Erysipelothrix rhusiopathiae (6.25%), Bacteroides spp. (13.54%), and Peptostreptococcus spp. (7.29%). In conclusion, normal vaginal bacterial flora of Criollo Limonero cows was predominantly Gram positive and included A. pyogenes, S. aureus, coagulase negative Staphylococcus, E. rhusiopathiae, Bacteroides spp., and Peptostreptococcus spp. In Criollo Limonero cattle, adaptive aspects such as development of humoral and physical mechanisms for defense, and bacterial adaptation to host deserve research attention.
Gasteiner, J; Horn, M; Steinwidder, A
This study was performed to investigate the effect of the transition from barn feeding to pasture on the pattern of reticuloruminal pH values in 8 multiparous dairy cows. A indwelling wireless data transmitting system for pH measurement was given to 8 multiparous cows orally. Reticuloruminal pH values were measured every 600 s over a period of 42 days. After 7 days of barn feeding (period 1), all of the animals were pastured with increasing grazing times from 2 to 7 h/day over 7 days (period 2). From day 15 to day 21 (period 3), the cows spent 7 h/day on pasture. Beginning on day 22, the animals had 20 h/day access to pasture (day and night grazing). To study reticuloruminal adaptation to pasture feeding, the phase of day and night grazing was subdivided into another 3 weekly periods (periods 4-6). Despite a mild transition period from barn feeding to pasture, significant effects on reticuloruminal pH values were observed. During barn feeding, the mean reticuloruminal pH value for all of the cows was 6.44 ± 0.14, and the pH values decreased significantly (p pH values increased again (pH 6.25 ± 0.22; pH 6.31 ± 0.17; pH 6.37 ± 0.16). Our results showed that the animals had significantly lowered reticuloruminal pH during the periods of feed transition from barn to pasture feeding. Despite these significant changes, the decrease was not harmful, as indicated by data of feed intake and milk production. Journal of Animal Physiology and Animal Nutrition © 2014 Blackwell Verlag GmbH.
Talukder, S; Ingenhoff, L; Kerrisk, K L; Celi, P
This study was conducted to examine the oxidative stress biomarkers in a cow diagnosed with a follicular cyst in her left ovary. Progesterone (P4) and plasma oxidative stress status was measured in 13 Holstein cows after synchronization of oestrus with controlled internal drug release (CIDR) and prostaglandinF2α (PGF2α) protocol. The presence and size of ovarian structures were monitored by transrectal ultrasound at 4 hourly intervals. Of the 13 cows, 12 were monitored until ovulation was detected and recorded, whereas one cow failed to ovulate and developed a follicular cyst. Oxidative stress biomarkers; reactive oxygen metabolites (ROMs), biological antioxidant potential (BAP), oxidative stress index (OSI), glutathione (GSH), ceruloplasmin and advanced oxidation protein products (AOPP) were measured in the cystic cow and compared to those of the 12 ovulated cows and are referred to as higher or lower if they are outside the mean ± standard error of mean of those of ovulated cows. The cystic cow had lower ROMs and OSI between 36 and 84 h after PGF2α injection and at 9 h, from 36 to 60 h after PGF2α injection respectively. On the other hand, antioxidant (BAP and GSH) was higher in the cystic cow compared to her ovulated herd mates. The observed imbalance between oxidant and antioxidant might have disrupted the physiological events for ovulation to occur, leading to cystic ovarian disease.
Cow's milk is increasingly suggested to play a role in the development of chronic degenerative, non-communicable disorders whereas goat's milk is advocated as having several health benefits. Cow's milk is a rich and cheap source of protein and calcium, and a valuable food for bone health. Despite their high content in saturated fats, consumption of full-fat dairy products does not seem to cause significant changes in cardiovascular disease risk variables. Early introduction of cow's milk is a strong negative determinant of iron status. Unmodified cow's milk does not meet nutritional requirements of infants although it is acceptable to add small volumes of cow's milk to complementary foods. Cow's milk protein allergy has a prevalence ranging from 2 to 7%, and the age of recovery is usually around 2-3 years. The evidence linking cow's milk intake to a later risk of type 1 diabetes or chronic degenerative, non-communicable disorders (obesity, metabolic syndrome, type 2 diabetes, hypertension) is not convincing. Milk probably protects against colorectal cancer, diets high in calcium are a probable cause of prostate cancer, and there is limited evidence suggesting that high consumption of milk and dairy products increases the risk for prostate cancer. There is no evidence to support the use of a cow's milk-free diet as a primary treatment for individuals with autistic spectrum disorders. Unmodified goat's milk is not suitable for infants because of the high protein and minerals content and of a low folate content. Goat's milk has no clear nutritional advantage over cow's milk and is not less allergenic. The European Food Safety Authority recently stated that proteins from goat's milk can be suitable as a protein source for infant and follow-on formula, provided the final product complies with the compositional criteria laid down in Directive 2006/141/EC. Copyright © 2013 S. Karger AG, Basel.
Naves, A B; Freitas Júnior, J E; Barletta, R V; Gandra, J R; Calomeni, G D; Gardinal, R; Takiya, C S; Vendramini, T H A; Mingoti, R D; Rennó, F P
Differing soya bean particle sizes may affect productive performance and ruminal fermentation due to the level of fatty acid (FA) exposure of the cotyledon in soya bean grain and because the protein in small particles is more rapidly degraded than the protein in large particles, which influence ruminal fibre digestion and the amounts of ruminally undegradable nutrients. The objective of this experiment was to investigate the effects of raw soya bean particle size on productive performance, digestion and milk FA profile of dairy cows. Twelve Holstein cows were assigned to three 4 × 4 Latin squares with 21-day periods. At the start of the experiment, cows were 121 days in milk (DIM) and yielded 30.2 kg/day of milk. Cows were fed 4 diets: (i) control diet (CO), without raw soya bean; (ii) whole raw soya bean (WRS); (iii) cracked raw soya bean in Wiley mill 4-mm screen (CS4); and (iv) cracked raw soya bean in Wiley mill 2-mm screen (CS2). The inclusion of soya beans (whole or cracked) was 200 g/kg on dry matter (DM) basis and partially replaced ground corn and soya bean meal. Uncorrected milk yield and composition were not influenced by experimental diets; however, fat-corrected milk (FCM) decreased when cows were fed soya bean treatments. Soya bean diets increased the intake of ether extract (EE) and net energy of lactation (NEL ), and decreased the intake of DM and non-fibre carbohydrate (NFC). Ruminal propionate concentration was lower in cows fed WRS than cows fed CS2 or CS4. Cows fed cracked raw soya bean presented lower nitrogen in faeces than cows fed WRS. The milk of cows fed WRS, CS2 and CS4 presented higher unsaturated FA than cows fed CO. The addition of raw soya bean in cow diets, regardless of the particle size, did not impair uncorrected milk yield and nutrient digestion, and increased the concentration of unsaturated FA in milk. Cows fed cracked raw soya bean presented similar productive performance to cows fed whole raw soya bean. Journal of
Bishop, Phillip A.; Fortney, Suzanne; Greenisen, Michael; Siconolfi, Steven F.; Bamman, Marcas M.; Moore, Alan D., Jr.; Squires, William
This manual describes the laboratory methods used to collect flight crew physiological performance data at the Johnson Space Center. The Exercise Countermeasures Project Laboratory is a standard physiology laboratory; only the application to the study of human physiological adaptations to spaceflight is unique. In the absence of any other recently published laboratory manual, this manual should be a useful document staffs and students of other laboratories.
Jorritsma, R.; Wensing, T.; Kruip, T.A.M.; Vos, P.L.A.M.; Noordhuizen, J.P.T.M.
This review addresses the suggestion that the decline in dairy reproductive performance, as increasingly observed these days, may be due to a hampered process of metabolic adaptation in early lactating cows. In our opinion, adaptation to the negative energy balance is a gradual process. Because
Gauly, M; Bollwein, H; Breves, G; Brügemann, K; Dänicke, S; Daş, G; Demeler, J; Hansen, H; Isselstein, J; König, S; Lohölter, M; Martinsohn, M; Meyer, U; Potthoff, M; Sanker, C; Schröder, B; Wrage, N; Meibaum, B; von Samson-Himmelstjerna, G; Stinshoff, H; Wrenzycki, C
It is well documented that global warming is unequivocal. Dairy production systems are considered as important sources of greenhouse gas emissions; however, little is known about the sensitivity and vulnerability of these production systems themselves to climate warming. This review brings different aspects of dairy cow production in Central Europe into focus, with a holistic approach to emphasize potential future consequences and challenges arising from climate change. With the current understanding of the effects of climate change, it is expected that yield of forage per hectare will be influenced positively, whereas quality will mainly depend on water availability and soil characteristics. Thus, the botanical composition of future grassland should include species that are able to withstand the changing conditions (e.g. lucerne and bird's foot trefoil). Changes in nutrient concentration of forage plants, elevated heat loads and altered feeding patterns of animals may influence rumen physiology. Several promising nutritional strategies are available to lower potential negative impacts of climate change on dairy cow nutrition and performance. Adjustment of feeding and drinking regimes, diet composition and additive supplementation can contribute to the maintenance of adequate dairy cow nutrition and performance. Provision of adequate shade and cooling will reduce the direct effects of heat stress. As estimated genetic parameters are promising, heat stress tolerance as a functional trait may be included into breeding programmes. Indirect effects of global warming on the health and welfare of animals seem to be more complicated and thus are less predictable. As the epidemiology of certain gastrointestinal nematodes and liver fluke is favourably influenced by increased temperature and humidity, relations between climate change and disease dynamics should be followed closely. Under current conditions, climate change associated economic impacts are estimated to be
Khelil-Arfa, H; Faverdin, P; Boudon, A
The aim of this study was to quantify the effect of the interaction between 2 constant ambient temperatures [thermoneutrality (TN; 15°C) and high temperature (HT; 28°C)] and 2 levels of Na bicarbonate supplementation [calculated to provide diet Na contents of 0.20%DM (Na-) and 0.50%DM (Na+)] on water partitioning in dairy cows. Treatments were compared on 4 dry and 4mid-lactation Holstein cows according to 2 Latin squares (1 for each physiological stage) over the course of 4 periods of 15d. Diets consisted of a total mixed ration based on maize silage. Dry cows were restricted to their protein and energy requirements, whereas lactating cows were fed ad libitum. The daily average temperature-humidity index was 59.4 for TN and 73.2 for HT. Lactating and dry cows had higher vaginal temperatures at HT than at TN, but the increase was more pronounced in lactating cows (+1.05 vs. +0.12°C for vaginal temperature, respectively). Dry matter intake (DMI) of lactating cows decreased by 2.3kg/d at HT. Free water intake (FWI) and estimated volume of water lost to evaporation increased at HT in both lactating and dry cows; no interactions were observed between temperature and physiological stage. When expressed as a proportion of DMI, the increase in evaporation that occurred with increasing temperature was completely compensated for by an increase in FWI for both physiological stages. The urinary water excretion increased slightly at HT in lactating cows but not in dry cows, which may be related to the low chloride content of the offered diet. High Na supplementation increased DMI slightly in lactating cows, but milk yield was not affected. Sodium supplementation did not limit the decrease in DMI observed in lactating cows at HT; this observation is likely due to the high diet electrolyte balance of the offered diets. Sodium supplementation increased FWI in lactating cows and urinary flow in both physiological states. The interaction between ambient temperature and Na
Autogenic Feedback Training Exercise (AFTE) is an protocol and technology developed by Dr. Patricia Cowings and her associates at NASA Ames Research Center as a means to facilitate astronaut adaptation to space and exposure to the microgravity. AFTE is a training method which involves teaching subjects to voluntarily control several of their own physiological responses to environmental stressors. As the procedures matured, the training program was expanded to determine if technology developed to facilitate astronaut adaptation to space would be valuable in treating patients suffering from autonomic and vestibular pathologies and symptomatic relief from nausea and/or blood pressure control anomalies such as hypo- or hypertension. The present study, performed in conjunction with Morehouse School of Medicine, Biomedical Engineering at The University of Akron and NASA Ames Research Center has demonstrated that this technology can be successfully applied over vast distances. The specific purpose of this research was to develop a PC based system which could handle processing of twenty channels of acquired physiological data in addition to the necessary duplex communication protocols that would, for example, permit a patient in Atlanta, GA to be trained by a clinician stationed in San Jose, CA. Sixteen channels of physiological data and 20 channels of processed data are included.
Nielsen, Bodil Højlund
and therefore has a major impact on economics and cow welfare. Moist and unhygienic conditions in the cows’ surroundings are considered as important risk factors for DD partly because this can disturb the skin barrier and make the animals more susceptible to infection and partly because the environment might...... act as an infection reservoir. Measures of cow leg cleanliness can be used as a proxy of the hygienic conditions on the floors. Nonetheless, only few studies have used direct measures of cleanliness in association with the risk of DD. Also, little is known about what factors can influence the cow leg...... cleanliness. More knowledge on these aspects will increase our understanding of the disease epidemiology and is essential to improve the success of controlling DD at the herd level. Therefore, the objectives of the present PhD thesis were 1) To investigate the relationship between cow leg cleanliness and DD...
Puppel, Kamila; Kuczyńska, Beata
The term 'metabolic profile' refers to the analysis of blood biochemical parameters that are useful to assess and prevent metabolic and nutritional disorders in dairy herds. In the higher standards of milk production, the priority in modern breeding is keeping dairy cows in high lactation and healthy. The proper analysis, as well as control. of their feeding and metabolic status is immensely important for the health condition of the herd. The disproportion between the genetically determined ability for milk production and the limitations in improving the energy value of the ration may be the cause of metabolic disorders. Negative energy balance has a major impact on the body's hormonal balance and organ functions and mostly appears during transition periods: from 3 to 2 weeks prepartum until 2-3 weeks postpartum. The term 'transition' is used to underscore the important physiological, metabolic and nutritional changes occurring in this time. The manner in which these changes occur and how they are diagnosed and detected are extremely important, as they are closely related to clinical and subclinical postpartum diseases, lactation and reproductive performance - factors that significantly shape the profitability of production. Therefore the priority for intensive milk production is prevention of metabolic diseases and other disorders. It is the intent of this review to synthesize and summarize the information currently available on metabolic status and physiological changes in the cow's body that occur during lactation, as well as to discuss the interpretation of the results, which will be a useful diagnostic tool in nutritional evaluations of the dairy herd. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.
Feuz, Dillon M; Umberger, Wendy J
Cow-calf production occurs in all 50 states over varied resource bases and under vastly different environmental conditions. Multiple breeds exist and management styles and objectives are as numerous as the number of cow-calf producers. There is not one area of the country, one breed of cattle, or one management style that is most profitable for producing cows and calves. There are, however, some common strategies that can be employed by cow-calf producers to enhance profitability. Costs need to be controlled without jeopardizing cow herd productivity or net returns. It appears that the cost associated with purchased and harvested feeds varies considerably across operations. Understanding cyclic and seasonal price patterns, weight-price slides, cattle shrink, and other marketing costs can help producers enhance their profit by marketing (and not by just selling) their cattle. Producers with superior cattle genetics can become part of a specific alliance or, at a minimum, document the performance of their cattle so that they can get paid for the superior genetics. The beef industry is changing and will likely continue to change. Cow-calf producers will need to examine their own management practices to determine whether they are optimal for the current industry. Those producers who are most adept at matching their management abilities to their cattle type, their resource base, and the appropriate market outlet will be the most successful in the future.
Adipose tissue is a central regulator of metabolism in dairy cows; however, little is known about the association between various proteins in adipose tissue and the metabolic status of peripartum cows. Therefore, the objectives were to (1) examine total protein expression in adipose tissue of dairy cows and (2) identify biomarkers in adipose that are linked to insulin resistance and to cows' metabolic status. Adipose tissue biopsies were obtained from eight multiparous cows at -17 and +4 days relative to parturition. Proteins were analyzed by intensity-based, label-free, quantitative shotgun proteomics (nanoLC-MS/MS). Cows were divided into groups with insulin-resistant (IR) and insulin-sensitive (IS) adipose according to protein kinase B phosphorylation following insulin stimulation. Cows with IR adipose lost more body weight postpartum compared with IS cows. Differential expression of 143 out of 586 proteins was detected in prepartum versus postpartum adipose. Comparing IR to IS adipose revealed differential expression of 18.9% of the proteins; those related to lipolysis (hormone-sensitive lipase, perilipin, monoglycerol lipase) were increased in IR adipose. In conclusion, we found novel biomarkers related to IR in adipose and to metabolic status that could be used to characterize high-yielding dairy cows that are better adapted to peripartum metabolic stress.
In dairy cattle, the increase in milk yield has been accompanied by a decrease in fertility and a more negative energy balance. As the hormone leptin is involved in regulation of nutritional status and reproductive function (Chapter 2) this is an
Liefers, S.C.; Veerkamp, R.F.; Pas, te M.F.W.; Chilliard, Y.; Lende, van der T.
In dairy cattle, the increase in milk yield has been accompanied by a more negative energy balance (EB) during early lactation and a decrease in fertility. As the hormone leptin is involved in regulation of nutritional status and reproductive function this hormone is an interesting protein to
Jones Bernt V
Full Text Available Abstract Background An elevated plasma glucose concentration has been considered to be a potential risk factor in the pathogenesis of left-displaced abomasums (DA. Therefore the present study was performed to investigate if spontaneous disease (parturient paresis, metritis, ketosis etc in dairy cows results in elevated concentrations of glucose and cortisol in blood as cortisol is the major regulator of glucose in ruminants. Methods Cortisol, insulin, β-hydroxybutyric acid (BHBA, non esterified fatty acids (NEFA, and serum calcium were analyzed in blood serum and glucose, in whole blood, from 57 spontaneously diseased cows collected at different farms. The cows were grouped according to the disease; parturient paresis, recumbent for other reasons, mastitis, metritis, ketosis, inappetance and others. Results No elevated concentrations of cortisol or glucose were found in cows with metritis and mastitis but both cortisol and glucose were elevated in cows stressed by recumbency. Cows with ketonemia (BHBA > 1.5 mmol/l did not have low concentration of glucose in blood but significantly low levels of cortisol. Some of these cows even had cortisol concentrations below the detection limit of the analysing method ( Conclusions The study gives patho-physiological support to the treatment strategies of ketosis, recommending glucocorticoids, insulin etc. However further studies of this problem are needed to understand why cows with ketosis have low levels of cortisol and normal levels of glucose. To what extent elevated cortisol and glucose levels in hypocalcemic and recumbent cows are involved in the ethiology and /or the pathogenesis of DA also will need further research.
This paper introduces the concept of personalized physiological medicine that is specifically directed at the needs of the critically ill patient. This differs from the conventional view of personalized medicine, characterized by biomarkers and gene profiling, instead focusing on time-variant changes in the pathophysiology and regulation of various organ systems and their cellular and subcellular constituents. I propose that personalized physiological medicine is composed of four pillars relevant to the critically ill patient. Pillar 1 is defined by the frailty and fitness of the patient and their physiological reserve to cope with the stress of critical illness and therapy. Pillar 2 involves monitoring of the key physiological variables of the different organ systems and their response to disease and therapy. Pillar 3 concerns the evaluation of the success of resuscitation by assessment of the hemodynamic coherence between the systemic and microcirculation and parenchyma of the organ systems. Finally, pillar 4 is defined by the integration of the physiological and clinical data into a time-learning adaptive model of the patient to provide feedback about the function of organ systems and to guide and assess the response to disease and therapy. I discuss each pillar and describe the challenges to research and development that will allow the realization of personalized physiological medicine to be practiced at the bedside for critically ill patients.
Fregonesi, J A; Veira, D M; von Keyserlingk, M A G; Weary, D M
Cows prefer to spend more time lying down in free stalls with more bedding, but no research to date has addressed the effects of bedding quality. Bedding in stalls often becomes wet either from exposure to the elements or from feces and urine. The aim of this study was to test the effect of wet bedding on stall preference and use. Four groups of 6 nonlactating Holstein cows were housed in free stalls bedded daily with approximately 0.1 m of fresh sawdust. Following a 5-d adaptation period, each group of cows was tested sequentially with access to stalls with either dry or wet sawdust bedding (86.4 +/- 2.1 vs. 26.5 +/- 2.1% dry matter), each for 2 d. These no-choice phases were followed by a 2-d free-choice phase during which cows had simultaneous access to stalls containing either wet or dry bedding. Stall usage was assessed by using 24-h video recordings scanned at 10-min intervals, and responses were analyzed by using a mixed model, with group (n = 4) as the observational unit. The minimum and maximum environmental temperatures during the experiment were 3.4 +/- 2.2 and 6.8 +/- 2.5 degrees C, respectively. When cows had access only to stalls with wet bedding, they spent 8.8 +/- 0.8 h/d lying down, which increased to 13.8 +/- 0.8 h/d when stalls with dry bedding were provided. Cows spent more time standing with their front 2 hooves in the stall when provided with wet vs. dry bedding (92 +/- 10 vs. 32 +/- 10 min/d). During the free-choice phase, all cows spent more time lying down in the dry stalls, spending 12.5 +/- 0.3 h/d in the dry stalls vs. 0.9 +/- 0.3 h/ d in stalls with wet bedding. In conclusion, dairy cows show a clear preference for a dry lying surface, and they spend much more time standing outside the stall when only wet bedding is available.
McConnel, Craig S; McNeil, Ashleigh A; Hadrich, Joleen C; Lombard, Jason E; Garry, Franklyn B; Heller, Jane
Over the past 175 years, data related to human disease and death have progressed to a summary measure of population health, the Disability-Adjusted Life Year (DALY). As dairies have intensified there has been no equivalent measure of the impact of disease on the productive life and well-being of animals. The development of a disease-adjusted metric requires a consistent set of disability weights that reflect the relative severity of important diseases. The objective of this study was to use an international survey of dairy authorities to derive disability weights for primary disease categories recorded on dairies. National and international dairy health and management authorities were contacted through professional organizations, dairy industry publications and conferences, and industry contacts. Estimates of minimum, most likely, and maximum disability weights were derived for 12 common dairy cow diseases. Survey participants were asked to estimate the impact of each disease on overall health and milk production. Diseases were classified from 1 (minimal adverse effects) to 10 (death). The data was modelled using BetaPERT distributions to demonstrate the variation in these dynamic disease processes, and to identify the most likely aggregated disability weights for each disease classification. A single disability weight was assigned to each disease using the average of the combined medians for the minimum, most likely, and maximum severity scores. A total of 96 respondents provided estimates of disability weights. The final disability weight values resulted in the following order from least to most severe: retained placenta, diarrhea, ketosis, metritis, mastitis, milk fever, lame (hoof only), calving trauma, left displaced abomasum, pneumonia, musculoskeletal injury (leg, hip, back), and right displaced abomasum. The peaks of the probability density functions indicated that for certain disease states such as retained placenta there was a relatively narrow range of
Farney, Jaymelynn K.; Mamedova, Laman K.; Coetzee, Johann F.; KuKanich, Butch; Sordillo, Lorraine M.; Stoakes, Sara K.; Minton, J. Ernest; Hollis, Larry C.
Adapting to the lactating state requires metabolic adjustments in multiple tissues, especially in the dairy cow, which must meet glucose demands that can exceed 5 kg/day in the face of negligible gastrointestinal glucose absorption. These challenges are met through the process of homeorhesis, the alteration of metabolic setpoints to adapt to a shift in physiological state. To investigate the role of inflammation-associated pathways in these homeorhetic adaptations, we treated cows with the nonsteroidal anti-inflammatory drug sodium salicylate (SS) for the first 7 days of lactation. Administration of SS decreased liver TNF-α mRNA and marginally decreased plasma TNF-α concentration, but plasma eicosanoids and liver NF-κB activity were unaltered during treatment. Despite the mild impact on these inflammatory markers, SS clearly altered metabolic function. Plasma glucose concentration was decreased by SS, but this was not explained by a shift in hepatic gluconeogenic gene expression or by altered milk lactose secretion. Insulin concentrations decreased in SS-treated cows on day 7 compared with controls, which was consistent with the decline in plasma glucose concentration. The revised quantitative insulin sensitivity check index (RQUICKI) was then used to assess whether altered insulin sensitivity may have influenced glucose utilization rate with SS. The RQUICKI estimate of insulin sensitivity was significantly elevated by SS on day 7, coincident with the decline in plasma glucose concentration. Salicylate prevented postpartum insulin resistance, likely causing excessive glucose utilization in peripheral tissues and hypoglycemia. These results represent the first evidence that inflammation-associated pathways are involved in homeorhetic adaptations to lactation. PMID:23678026
The physiological characteristics of man depend on the intake, metabolism and excretion of stable elements from food, water, and air. The physiological behavior of natural radionuclides and radionuclides from nuclear weapons testing and from the utilization of nuclear energy is believed to follow the pattern of stable elements. Hence information on the normal physiological processes occurring in the human body plays an important role in the assessment of the radiation dose received by man. Two important physiological parameters needed for internal dose determination are the pulmonary function and the water balance. In the Coordinated Research Programme on the characterization of Asian population, five participants submitted data on these physiological characteristics - China, India, Japan, Philippines and Viet Nam. During the CRP, data on other pertinent characteristics such as physical and dietary were simultaneously being collected. Hence, the information on the physiological characteristics alone, coming from the five participants were not complete and are probably not sufficient to establish standard values for the Reference Asian Man. Nonetheless, the data collected is a valuable contribution to this research programme
Boer, H M T; Butler, S T; Stötzel, C; Te Pas, M F W; Veerkamp, R F; Woelders, H
A recently developed mechanistic mathematical model of the bovine estrous cycle was parameterized to fit empirical data sets collected during one estrous cycle of 31 individual cows, with the main objective to further validate the model. The a priori criteria for validation were (1) the resulting model can simulate the measured data correctly (i.e. goodness of fit), and (2) this is achieved without needing extreme, probably non-physiological parameter values. We used a least squares optimization procedure to identify parameter configurations for the mathematical model to fit the empirical in vivo measurements of follicle and corpus luteum sizes, and the plasma concentrations of progesterone, estradiol, FSH and LH for each cow. The model was capable of accommodating normal variation in estrous cycle characteristics of individual cows. With the parameter sets estimated for the individual cows, the model behavior changed for 21 cows, with improved fit of the simulated output curves for 18 of these 21 cows. Moreover, the number of follicular waves was predicted correctly for 18 of the 25 two-wave and three-wave cows, without extreme parameter value changes. Estimation of specific parameters confirmed results of previous model simulations indicating that parameters involved in luteolytic signaling are very important for regulation of general estrous cycle characteristics, and are likely responsible for differences in estrous cycle characteristics between cows.
Full Text Available Composition and physico-chemical properties of buffalo and cow milks were compared at their initial pH and during chemical acidification. As compare to cow milk, buffalo milk was richer in caseins and minerals such as Ca, Mg and Pi. Along with these differences, the capacity of buffalo milk to be acidified named buffering capacity was also higher than cow milk. The pH decrease induced aggregation of caseins at their isoelectric pH and solubilisations of Ca and Pi were significant for both milks. For both species, these molecular changes were qualitatively similar but quantitatively different. These differences suggested that the acidification process in dairy technology which is well established for cow milk can not be directly extrapolated to buffalo milk and some adaptations are necessary.
Full Text Available Cows exposed to heat stress exhibit a decreased ability to mobilize lipids due to increased sensitivity to insulin, which is expressed in a decreased concentration of NEFA. However, certain cows can preserve the level of lipid mobilization after adapting to heat stress. We assumed that cows that have a preserved ability to mobilize lipids are less sensitive to insulin and that they have a lower tolerance for glucose. The aim of this work was to compare the results of an intravenous glucose tolerance test in cows that exhibited, in prolonged heat stress, a decreased (NEFA0.30 mmol/l ability for lipid mobilization. Glucose concentration and NEFA concentration were measured following intravenous application of glucose. The mean glycaemic index value did not differ statistically significantly between the two groups of cows at 10, 15 and 20 minutes after glucose application (p>0.05, but there was a tendency at 10 and 15 minutes for the glycaemia to be higher in cows with preserved lipomobilization (p<0.1. At 30, 60 and 90 minutes after glucose application, glycaemia was statistically significantly higher (p<0.01; p<0.05 and p<0.05 in the group of cows with preserved lipomobilization. The glycaemic index values (mmol/l shown in the same order (30, 60 and 90 minutes were as follows 9.91±0.21: 9.23±0.41; 5.41±0.5: 4.67±0.33 and 4.31±0.39: 3.47±0.37. The mean value for NEFA concentration in samples originating from the two experimental groups of cows did not differ statistically significantly following glucose application. The NEFA concentration showed a tendency to be higher in cows with preserved lipid mobilization in comparison with cows with decreased lipomobilization at 20 and 30 minutes after glucose application (p<0.1. Following the intravenous glucose tolerance test, NEFA and glucose concentrations were in a significant negative correlation, and that correlation was more expressed in cows with decreased lipomobilization. Cows with preserved
Buchheit, M; Voss, S C; Nybo, Lars
The aim of the present study was to examine the associations between adaptive responses to an in-season soccer training camp in the heat and changes in submaximal exercising heart rate (HRex, 5-min run at 9 ¿km/h), postexercise HR recovery (HRR) and HR variability (HRV). Fifteen well-trained but ......The aim of the present study was to examine the associations between adaptive responses to an in-season soccer training camp in the heat and changes in submaximal exercising heart rate (HRex, 5-min run at 9 ¿km/h), postexercise HR recovery (HRR) and HR variability (HRV). Fifteen well......-trained but non-heat-acclimatized male adult players performed a training week in Qatar (34.6¿±¿1.9°C wet bulb globe temperature). HRex, HRR, HRV (i.e. the standard deviation of instantaneous beat-to-beat R-R interval variability measured from Poincaré plots SD1, a vagal-related index), creatine kinase (CK...... at the beginning and at the end of the training week. Throughout the intervention, HRex and HRV showed decreasing (P¿...
Steeneveld, W.; Vernooij, J.C.M.; Hogeveen, H.
To improve management on dairy herds, sensor systems have been developed that can measure physiological, behavioral, and production indicators on individual cows. It is not known whether using sensor systems also improves measures of health and production in dairy herds. The objective of this study
Steeneveld, W.; Vernooij, J.C.M.; Hogeveen, H.
To improve management on dairy herds, sensor systems have been developed that can measure physiological, behavioral, and production indicators on individual cows. It is not known whether using sensor systems also improves measures of health and production in dairy herds. The objective of this study
Larsen, Mogens; Kristensen, Niels Bastian
-organ transfer of nitrogen from catabolised AA. AAs seem to be prioritised for anabolic purposes, indicating the relevance of investigating effects of supplying additional protein to post partum dairy cows. Combining data from quantitative and qualitative experimental techniques on L-lactate metabolism point...... to the conclusion that the quantitatively most important adaptation of metabolism to support the increased glucose demand in the immediate post partum period is endogenous recycling of glucogenic carbon through lactate. This is mediated by a dual site of adaptation of metabolism in the liver and in the peripheral...
Full Text Available The objectives of this study were to evaluate the effects of different supplemental levels of Saccharomyces cerevisiae fermentation product (SCFP; Original XP; Diamond V on lactation performance in Holstein dairy cows under heat stress. Eighty-one multiparous Holstein dairy cows were divided into 27 blocks of 3 cows each based on milk yield (23.6±0.20 kg/d, parity (2.88±0.91 and day in milk (204±46 d. The cows were randomly assigned within blocks to one of three treatments: 0 (control, 120, or 240 g/d of SCFP mixed with 240, 120, or 0 g of corn meal, respectively. The experiment was carried out during the summer season of 2014, starting from 14 July 2014 and lasting for 9 weeks with the first week as adaption period. During the experimental period, average daily temperature-humidity index (measured at 08:00, 14:00, and 20:00 was above 68, indicating that cows were exposed to heat stress throughout the study. Rectal temperatures tended to decrease linearly (p = 0.07 for cows supplemented with SCFP compared to the control cows at 14:30, but were not different at 06:30 (p>0.10. Dry matter intake was not affected by SCFP supplementation (p>0.10. Milk yield increased linearly (p0.10 was observed among the treatments in conversion of dietary crude protein to milk protein yield. In summary, supplementation of SCFP alleviated the negative effect of heat stress in lactating Holstein dairy cows and allowed cows to maintain higher milk production, feed efficiency and net energy balance. Effects of SCFP were dose-dependent and greater effects were observed from higher doses.
Background Pichia pastoris is an established eukaryotic host for the production of recombinant proteins. Most often, protein production is under the control of the strong methanol-inducible aox1 promoter. However, detailed information about the physiological alterations in P. pastoris accompanying the shift from growth on glycerol to methanol-induced protein production under industrial relevant conditions is missing. Here, we provide an analysis of the physiological response of P. pastoris GS115 to methanol-induced high-level production of the Hepatitis B virus surface antigen (HBsAg). High product titers and the retention of the protein in the endoplasmic reticulum (ER) are supposedly of major impact on the host physiology. For a more detailed understanding of the cellular response to methanol-induced HBsAg production, the time-dependent changes in the yeast proteome and ultrastructural cell morphology were analyzed during the production process. Results The shift from growth on glycerol to growth and HBsAg production on methanol was accompanied by a drastic change in the yeast proteome. In particular, enzymes from the methanol dissimilation pathway started to dominate the proteome while enzymes from the methanol assimilation pathway, e.g. the transketolase DAS1, increased only moderately. The majority of methanol was metabolized via the energy generating dissimilatory pathway leading to a corresponding increase in mitochondrial size and numbers. The methanol-metabolism related generation of reactive oxygen species induced a pronounced oxidative stress response (e.g. strong increase of the peroxiredoxin PMP20). Moreover, the accumulation of HBsAg in the ER resulted in the induction of the unfolded protein response (e.g. strong increase of the ER-resident disulfide isomerase, PDI) and the ER associated degradation (ERAD) pathway (e.g. increase of two cytosolic chaperones and members of the AAA ATPase superfamily) indicating that potential degradation of HBsAg could
Davis, Amanda N; Clegg, J L; Perry, C A; McFadden, J W
The progression of insulin resistance in dairy cows represents a maternal adaptation to support milk production during heightened energy demand; however, excessive adipose tissue lipolysis can develop. In diabetic non-ruminants, the mechanisms that mediate insulin resistance involve the sphingolipid ceramide. We tested the hypothesis that ceramide accumulates in dairy cows experiencing lipolysis and insulin resistance. Nine dairy cows were utilized in a replicated 3 × 3 Latin square design. Cows were ad libitum fed, nutrient-restricted (NR), or NR with nicotinic acid (NA; 5 mg of NA/h per kg BW; delivered i.v.) for 34 h. When provided access, cows were ad libitum fed a mixed ration of grass hay and ground corn to meet requirements. Intake for NR cows was limited to vitamins and minerals. Nicotinic acid was administered to suppress lipolysis. Saline was infused in cows not provided NA. At 32 and 33 h of treatment, a liver biopsy and insulin tolerance test were performed, respectively. Samples were analyzed using colorimetry, immunoassay, and mass spectrometry. Nutrient restriction increased serum fatty acids and ceramide levels, and impaired insulin sensitivity; however, NA infusion was unable to prevent these responses. We also show that NR increases hepatic ceramide accumulation, a response that was positively associated with serum ceramide supply. Our data demonstrate that circulating and hepatic 24:0-Cer are inversely associated with systemic insulin tolerance, an effect not observed for the 16:0 moiety. In conclusion, our results suggest that ceramide accrual represents a metabolic adaptation to nutrient restriction and impaired insulin action in dairy cows.
Jimenez Infante, Francy M.
Given the high salinity, prevailing annual high temperatures, and ultra-oligotrophic conditions in the Red Sea isolation and characterization of important microbial groups thriving in this environment is important in understanding the ecological significance and metabolic capabilities of these communities. By using a high-throughput cultivation technique in natural seawater amended with minute amounts of nutrients, members of the rare biosphere (PS1), methylotrophic Betaproteobacteria (OM43), and the ubiquitous and abundant SAR11 group (Pelagibacterales), were isolated in pure culture. Phylogenetic analyses of Red Sea isolates along with comparative genomics with close representatives from disparate provinces revealed ecotypes and genomic differentiation among the groups. Firstly, the PS1 alphaproteobacterial clade was found to be present in very low abundance in several metagenomic datasets form divergent environments. While strain RS24 (Red Sea) harbored genomic islands involved in polymer degradation, IMCC14465 (East (Japan) Sea) contained unique genes for degradation of aromatic compounds. Secondly, methylotrophic OM43 bacteria from the Red Sea (F5, G12 and H7) showed higher similarities with KB13 isolate from Hawaii, forming a ‘H-RS’ (Hawaii-Red Sea) cluster separate from HTCC2181 (Oregon isolate). HTCC2181 members were shown to prevail in cold, productive coastal environments and had an nqrA-F system for energy generation by sodium motive force. On the contrary, H-RS cluster members may be better adapted to warm and oligotrophic environments, and seem to generate energy by using a proton-translocating NADH:Quinone oxidoreductase (complex I; nuoA-N subunits). Moreover, F5, G12, and H7 had unique proteins related to resistance to UV, temperature and salinity, in addition to a heavy metal ‘resistance island’ as adaptive traits to cope with the environmental conditions in the Red Sea. Finally, description of the Red Sea Pelagibacterales
Schindlbacher, Andreas; Schnecker, Jörg; Takriti, Mounir; Borken, Werner; Wanek, Wolfgang
Thermal adaptations of soil microorganisms could mitigate or facilitate global warming effects on soil organic matter (SOM) decomposition and soil CO2 efflux. We incubated soil from warmed and control subplots of a forest soil warming experiment to assess whether 9 years of soil warming affected the rates and the temperature sensitivity of the soil CO2 efflux, extracellular enzyme activities, microbial efficiency, and gross N mineralization. Mineral soil (0-10 cm depth) was incubated at temperatures ranging from 3 to 23 °C. No adaptations to long-term warming were observed regarding the heterotrophic soil CO2 efflux (R10 warmed: 2.31 ± 0.15 μmol m(-2) s(-1) , control: 2.34 ± 0.29 μmol m(-2) s(-1) ; Q10 warmed: 2.45 ± 0.06, control: 2.45 ± 0.04). Potential enzyme activities increased with incubation temperature, but the temperature sensitivity of the enzymes did not differ between the warmed and the control soils. The ratio of C : N acquiring enzyme activities was significantly higher in the warmed soil. Microbial biomass-specific respiration rates increased with incubation temperature, but the rates and the temperature sensitivity (Q10 warmed: 2.54 ± 0.23, control 2.75 ± 0.17) did not differ between warmed and control soils. Microbial substrate use efficiency (SUE) declined with increasing incubation temperature in both, warmed and control, soils. SUE and its temperature sensitivity (Q10 warmed: 0.84 ± 0.03, control: 0.88 ± 0.01) did not differ between warmed and control soils either. Gross N mineralization was invariant to incubation temperature and was not affected by long-term soil warming. Our results indicate that thermal adaptations of the microbial decomposer community are unlikely to occur in C-rich calcareous temperate forest soils. © 2015 The Authors. Global Change Biology published by John Wiley & Sons Ltd.
Full Text Available The purpose of this study was to investigate the eco-physiological adaptation of indigenous woody species to their habitats in karst areas of southwestern China. Two contrasting forest habitats were studied: a degraded habitat in Daxiagu and a well-developed habitat in Tianlongshan, and the eco-physiological characteristics of the trees were measured for three growth seasons. Photosynthetic rate (Pn, stomatal conductance (gs, and transpiration rate (Tr of the tree species in Daxiagu were 2-3 times higher than those in Tianlongshan under ambient conditions. However, this habitat effect was not significant when measurements were taken under controlled conditions. Under controlled conditions, Pn, gs, and Tr of the deciduous species were markedly higher than those for the evergreen species. Habitat had no significant effect on water use efficiency (WUE or photochemical characteristics of PSII. The stomatal sensitivity of woody species in the degraded habitat was much higher than that in the well-developed habitat. Similarly, the leaf total nitrogen (N and phosphorus (P contents expressed on the basis of either dry mass or leaf area were also much higher in Daxiagu than they were in Tianlongshan. The mass-based leaf total N content of deciduous species was much higher than that of evergreen species, while leaf area-based total N and P contents of evergreens were significantly higher than those of deciduous species. The photosynthetic nitrogen- and phosphorus-use efficiencies (PNUE and PPUE of deciduous species were much higher than those of evergreens. Further, the PPUE of the woody species in Tianlongshan was much higher than that of the woody species in Daxiagu. The results from three growth seasons imply that the tree species were able to adapt well to their growth habitats. Furthermore, it seems that so-called “temporary drought stress” may not occur, or may not be severe for most woody plants in karst areas of southwestern China.
Full Text Available The purpose of this study was to investigate the eco-physiological adaptation of indigenous woody species to their habitats in karst areas of southwestern China. Two contrasting forest habitats were studied: a degraded habitat in Daxiagu and a well-developed habitat in Tianlongshan, and the eco-physiological characteristics of the trees were measured for three growth seasons. Photosynthetic rate (Pn, stomatal conductance (gs, and transpiration rate (Tr of the tree species in Daxiagu were 2-3 times higher than those in Tianlongshan under ambient conditions. However, this habitat effect was not significant when measurements were taken under controlled conditions. Under controlled conditions, Pn, gs, and Tr of the deciduous species were markedly higher than those for the evergreen species. Habitat had no significant effect on water use efficiency (WUE or photochemical characteristics of PSII. The stomatal sensitivity of woody species in the degraded habitat was much higher than that in the well-developed habitat. Similarly, the leaf total nitrogen (N and phosphorus (P contents expressed on the basis of either dry mass or leaf area were also much higher in Daxiagu than they were in Tianlongshan. The mass-based leaf total N content of deciduous species was much higher than that of evergreen species, while leaf area-based total N and P contents of evergreens were significantly higher than those of deciduous species. The photosynthetic nitrogen- and phosphorus-use efficiencies (PNUE and PPUE of deciduous species were much higher than those of evergreens. Further, the PPUE of the woody species in Tianlongshan was much higher than that of the woody species in Daxiagu. The results from three growth seasons imply that the tree species were able to adapt well to their growth habitats. Furthermore, it seems that so-called “temporary drought stress” may not occur, or may not be severe for most woody plants in karst areas of southwestern China.
Daga, Pankaj R; Bolger, Michael B; Haworth, Ian S; Clark, Robert D; Martin, Eric J
When medicinal chemists need to improve bioavailability (%F) within a chemical series during lead optimization, they synthesize new series members with systematically modified properties mainly by following experience and general rules of thumb. More quantitative models that predict %F of proposed compounds from chemical structure alone have proven elusive. Global empirical %F quantitative structure-property (QSPR) models perform poorly, and projects have too little data to train local %F QSPR models. Mechanistic oral absorption and physiologically based pharmacokinetic (PBPK) models simulate the dissolution, absorption, systemic distribution, and clearance of a drug in preclinical species and humans. Attempts to build global PBPK models based purely on calculated inputs have not achieved the optimization. In this work, local GastroPlus PBPK models are instead customized for individual medchem series. The key innovation was building a local QSPR for a numerically fitted effective intrinsic clearance (CL loc ). All inputs are subsequently computed from structure alone, so the models can be applied in advance of synthesis. Training CL loc on the first 15-18 rat %F measurements gave adequate predictions, with clear improvements up to about 30 measurements, and incremental improvements beyond that.
Hu, Y. [Mayo Clinic Arizona (United States)
MRI-guided treatment is a growing area of medicine, particularly in radiotherapy and surgery. The exquisite soft tissue anatomic contrast offered by MRI, along with functional imaging, makes the use of MRI during therapeutic procedures very attractive. Challenging the utility of MRI in the therapy room are many issues including the physics of MRI and the impact on the environment and therapeutic instruments, the impact of the room and instruments on the MRI; safety, space, design and cost. In this session, the applications and challenges of MRI-guided treatment will be described. The session format is: Past, present and future: MRI-guided radiotherapy from 2005 to 2025: Jan Lagendijk Battling Maxwell’s equations: Physics challenges and solutions for hybrid MRI systems: Paul Keall I want it now!: Advances in MRI acquisition, reconstruction and the use of priors to enable fast anatomic and physiologic imaging to inform guidance and adaptation decisions: Yanle Hu MR in the OR: The growth and applications of MRI for interventional radiology and surgery: Rebecca Fahrig Learning Objectives: To understand the history and trajectory of MRI-guided radiotherapy To understand the challenges of integrating MR imaging systems with linear accelerators To understand the latest in fast MRI methods to enable the visualisation of anatomy and physiology on radiotherapy treatment timescales To understand the growing role and challenges of MRI for image-guided surgical procedures My disclosures are publicly available and updated at: http://sydney.edu.au/medicine/radiation-physics/about-us/disclosures.php.
Full Text Available The social network game Farmville, which allows players to grow crops, raise animals, and produce a variety of goods, proved enormously successful within a year of its launch in 2009, attracting 110 million Facebook users. However, the game has been criticised for its mindless mechanics, which require little more than repeated clicking on its colourful icons. By way of parody, Ian Bogost’s Cow Clicker permits its players to simply click on a picture of a cow once every six hours. In this essay I extend Bogost’s critique and suggest that Cow Clicker highlights not just the soulless inanity of Farmville gameplay but also the paucity of that game’s portrayal of the painful reality of a dairy cow’s punishing daily existence and untimely end.
Nielsen, Bodil Højlund
cleanliness was explored but no effect was found. In the second study, potential herd and cow level risk factors for poor hind leg cleanliness were evaluated. Data were obtained from a cross sectional study in 42 commercial dairy herds conducted by senior scientist Peter T. Thomsen. Here, no access to pasture......Digital dermatitis (DD) is an infectious cattle disease presumably caused by Treponema spp. It results in painful, ulcerative lesions in the skin of the distal extremities and can be associated with lameness in affected animals. Today, DD is a very prevalent disease in the dairy industry......; 2) To identify potential risk factors for poor cow leg cleanliness; and 3) To gain more knowledge about potential means of controlling DD. Data was obtained from three studies conducted in commercial Danish dairy herds and the results are presented in four scientific papers. In the first study, cow...
Lane, Helen W.; Whitson, Peggy A.; Putcha, Lakshmi; Baker, Ellen; Smith, Scott M.; Stewart, Karen; Gretebeck, Randall; Nimmagudda, R. R.; Schoeller, Dale A.; Davis-Street, Janis
As noted elsewhere in this report, a central goal of the Extended Duration Orbiter Medical Project (EDOMP) was to ensure that cardiovascular and muscle function were adequate to perform an emergency egress after 16 days of spaceflight. The goals of the Regulatory Physiology component of the EDOMP were to identify and subsequently ameliorate those biochemical and nutritional factors that deplete physiological reserves or increase risk for disease, and to facilitate the development of effective muscle, exercise, and cardiovascular countermeasures. The component investigations designed to meet these goals focused on biochemical and physiological aspects of nutrition and metabolism, the risk of renal (kidney) stone formation, gastrointestinal function, and sleep in space. Investigations involved both ground-based protocols to validate proposed methods and flight studies to test those methods. Two hardware tests were also completed.
There has been a long history of interaction between mathematics and physiology. This book looks in detail at a wide selection of mathematical models in physiology, showing how physiological problems can be formulated and studied mathematically, and how such models give rise to interesting and challenging mathematical questions. With its coverage of many recent models it gives an overview of the field, while many older models are also discussed, to put the modern work in context. In this second edition the coverage of basic principles has been expanded to include such topics as stochastic differential equations, Markov models and Gibbs free energy, and the selection of models has also been expanded to include some of the basic models of fluid transport, respiration/perfusion, blood diseases, molecular motors, smooth muscle, neuroendrocine cells, the baroreceptor loop, turboglomerular oscillations, blood clotting and the retina. Owing to this extensive coverage, the second edition is published in two volumes. ...
Objective refraction through plus fogging lenses and base-in prisms revealed that normally accommodation is not completely relaxed when the stimulus to accommodation is zero. The myopic shift in the refractive error due to this focus error of accommodation was defined as physiological pseudomyopia. Two previously established features of accommodation are responsible for this behavior: (1) accommodation acts as a proportional control system for steady-state responses; and (2) the rest focus of accommodation is nonzero. It is proposed that the hyperopic shift in refraction observed in cycloplegia is the result of elimination of physiological pseudomyopia.
Grellet-Tinner, G; Lindsay, S; Thompson, M B
Megapodes are galliform birds endemic to Australasia and unusual among modern birds in that they bury their eggs for incubation in diverse substrates and using various strategies. Alectura lathami and Leipoa ocellata are Australian megapodes that build and nest in mounds of soil and organic matter. Such unusual nesting behaviours have resulted in particular evolutionary adaptations of their eggs and eggshells. We used a combination of scanning electron microscopy, including electron backscatter diffraction and energy-dispersive X-ray spectroscopy, to determine the fine structure of the eggshells and micro-CT scanning to map the structure of pores. We discovered that the surface of the eggshell of A. lathami displays nodes similar to those of extinct titanosaur dinosaurs from Transylvania and Auca Mahuevo egg layer #4. We propose that this pronounced nodular ornamentation is an adaptation to an environment rich in organic acids from their nest mound, protecting the egg surface from chemical etching and leaving the eggshell thickness intact. By contrast, L. ocellata nests in mounds of sand with less organic matter in semiarid environments and has eggshells with weakly defined nodes, like those of extinct titanosaurs from AM L#3 that also lived in a semiarid environment. We suggest the internode spaces in both megapode and titanosaur species act as funnels, which concentrate the condensed water vapour between the nodes. This water funnelling in megapodes through the layer of calcium phosphate reduces the likelihood of bacterial infection by creating a barrier to microbial invasion. In addition, the accessory layer of both species possesses sulphur, which reinforces the calcium phosphate barrier to bacterial and fungal contamination. Like titanosaurs, pores through the eggshell are Y-shaped in both species, but A. lathami displays unique mid-shell connections tangential to the eggshell surface and that connect some adjacent pores, like the eggshells of titanosaur of AM
Moriel, P; Scatena, T S; Sá Filho, O G; Cooke, R F; Vasconcelos, J L M
Two experiments were conducted to investigate the effects of carbohydrate source and processing on serum progesterone (P4) and insulin concentrations of nonlactating dairy cows. In experiment 1, 12 ovariectomized grazing Gir x Holstein cows were stratified by body weight and body condition score, and randomly assigned to receive a supplement containing either finely ground corn or citrus pulp in a Latin square crossover design. Diets were fed individually, twice daily at a rate of 10.9 kg of dry matter per cow. Cows received a controlled intravaginal P4-releasing insert before the beginning of the study, and inserts were replaced every 7 d. During the first experimental period, cows were adapted to treatments from d 0 to 13 and blood was collected on d 14, whereas during the second experimental period cows were adapted to treatments from d 0 to 6 and blood samples were collected on d 7. In both periods, blood samples were collected immediately before and at 1, 2, 3, 4, 5, and 6 h after the first supplement feeding of the collection day. In experiment 2, the cows utilized in experiment 1 were randomly assigned to receive a supplement based on finely ground corn, coarsely ground corn, or high-moisture corn in a Latin square crossover design. Cows were fed and received the controlled intravaginal P4-releasing insert as in experiment 1. Within each of the 3 experimental periods, cows were adapted to diets from d 0 to 6, and blood samples were collected on d 7 as in experiment 1. Time effects were detected in experiments 1 and 2 because insulin concentrations increased by 1 h (4.6 +/- 0.90 vs. 7.4 +/- 0.91 microIU/mL for 0 and 1 h, respectively) and P4 concentrations decreased by 3 h (1.8 +/- 0.12 vs. 1.2 +/- 0.11 ng/mL for 0 and 3 h, respectively) after supplements were offered. In experiment 2, insulin concentrations were greater in cows fed high-moisture corn compared with those fed coarsely or finely ground corn (8.8 +/- 1.05, 5.7 +/- 1.05, and 6.1 +/- 1.05 micro
Full text: The rumen is an important part of the digestive tract of ruminant animals such as cattle, buffalo, sheep and goats. It contains large numbers of micro-organisms whose function is to break down fibrous feed materials such as grass and straw and convert them to products that can be used by the animal to produce meat, milk, wool or draught power. To study the microbial population of the rumen under controlled laboratory conditions, Dr. J.W. Czerkawski of the Hannah Research Institute, Scotland, U.K., developed an 'artificial cow'. The 'cow', named RUSITEC (from the acronym of 'Rumen Simulation Technique') is today being used as part of a project to analyse different feedstuffs being carried out by the Food and Agriculture Organization of the United Nations (FAO) and the International Atomic Energy Agency (IAEA) at their joint Agricultural Laboratory at Seibersdorf near Vienna, Austria. In the artificial rumen micro-organisms can be indefinitely maintained by feeding a normal ruminant diet each day and providing the correct physiological conditions in terms of temperature, pH and flow of saliva. As RUSITEC chews its way through different feeds, scientists use radioactive tracing techniques to compare their digestibility. (The higher the digestibility of a foodstuff, the higher the nutritive value that can be derived from it.) By analysing the quality of different feeding materials in this way, scientists are seeking to propose improved diets for domestic animals in the developing world. Photos on this page show RUSITEC at work. Below, the vessels representing the rumen, where microbial fermentation of diets takes place; right, the rumen simulation technique in operation; below right, analysis of the end products of fermentative digestion.
Vitali, A; Felici, A; Esposito, S; Bernabucci, U; Bertocchi, L; Maresca, C; Nardone, A; Lacetera, N
This study investigated the mortality of dairy cows during heat waves. Mortality data (46,610 cases) referred to dairy cows older than 24mo that died on a farm from all causes from May 1 to September 30 during a 6-yr period (2002-2007). Weather data were obtained from 12 weather stations located in different areas of Italy. Heat waves were defined for each weather station as a period of at least 3 consecutive days, from May 1 to September 30 (2002-2007), when the daily maximum temperature exceeded the 90th percentile of the reference distribution (1971-2000). Summer days were classified as days in heat wave (HW) or not in heat wave (nHW). Days in HW were numbered to evaluate the relationship between mortality and length of the wave. Finally, the first 3 nHW days after the end of a heat wave were also considered to account for potential prolonged effects. The mortality risk was evaluated using a case-crossover design. A conditional logistic regression model was used to calculate odds ratio and 95% confidence interval for mortality recorded in HW compared with that recorded in nHW days pooled and stratified by duration of exposure, age of cows, and month of occurrence. Dairy cows mortality was greater during HW compared with nHW days. Furthermore, compared with nHW days, the risk of mortality continued to be higher during the 3 d after the end of HW. Mortality increased with the length of the HW. Considering deaths stratified by age, cows up to 28mo were not affected by HW, whereas all the other age categories of older cows (29-60, 61-96, and >96mo) showed a greater mortality when exposed to HW. The risk of death during HW was higher in early summer months. In particular, the highest risk of mortality was observed during June HW. Present results strongly support the implementation of adaptation strategies which may limit heat stress-related impairment of animal welfare and economic losses in dairy cow farm during HW. Copyright © 2015 American Dairy Science
Gessner, D K; Most, E; Schlegel, G; Kupczyk, K; Schwarz, F J; Eder, K
This study was performed to investigate the hypothesis that supplementation of conjugated linoleic acid (CLA) changes the concentrations of retinol and tocopherols in the milk of cows. To investigate this hypothesis, Holstein cows received daily from 3 weeks ante-partum to 14 weeks post-partum either 172 g of a CLA-free rumen-protected control fat (control group, n = 20) or the same amount of a rumen-protected CLA fat, supplying 4.3 g of cis-9, trans-11 CLA and 3.8 g of trans-10, cis-12 CLA per d (CLA group, n = 20). Milk samples (collected at weeks 1, 3, 5, 8 and 11 of lactation) were analysed for retinol, α- and γ-tocopherol concentrations. Milk of cows supplemented with CLA had higher concentrations of retinol (+34%), α-tocopherol (+44%) and γ-tocopherol (+21%) than milk of control cows (p tocopherol and γ-tocopherol, respectively, p tocopherols, concentrations of thiobarbituric acid-reactive substances, determined in milk of week 5, were lower in cows of the CLA group than in control cows, indicative of a lower susceptibility of milk lipids to peroxidation. Plasma concentrations of retinol and α-tocopherol, determined at 1 and 5 weeks post-partum, were not different between the two groups of cows. In conclusion, this study shows that supplementing dairy cows with a moderate amount of CLA causes an increase of the concentrations of vitamins A and E in the milk and results in an increased output of those vitamins via milk. These effects might be beneficial with respect to the nutritional value of dairy products and the susceptibility of milk fat to oxidative deterioration. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.
Rodrigues, P F; Menezes, L M; Azambuja, R C C; Suñé, R W; Barbosa Silveira, I D; Cardoso, F F
This study assessed milk yield and composition of Angus and Angus-cross beef cows raised in southern Brazil. A total of 128 records were collected in 2 consecutive calving seasons from cows between 3 and 5 yr of age of 4 breed compositions: Angus (ANAN), Caracu × Angus (CRAN), Hereford × Angus (HHAN), and Nelore × Angus (NEAN). These cows were mated to Brangus (BN) or Braford (BO) bulls and managed under extensive grazing conditions in southern Brazil. Milk production of these cows was assessed by 2 procedures: indirectly by the calf weigh-suckle-weigh procedure (WD) and directly by machine milking (MM). Lactation curves were estimated using nonlinear regression and the following related traits were derived: peak yield (PY), peak week (PW), total yield at 210 d (TY210), and lactation persistence (PERS). Milk composition and calf weaning weight adjusted to 210 d (WW210) were also determined. The MM technique was considered more accurate because of lower standard errors of estimated means, greater statistical power, and greater correlation between TY210 and WW210 (0.50) compared to WD (0.36). Considering the more precise evaluation by MM, the CRAN and NEAN cows had greater TY210 (1070 and 1116 kg, respectively) and PY (8.1 and 7.8 kg, respectively) compared to ANAN and HHAN cows, which had 858 and 842 kg for TY210 and 6.6 and 6.3 kg for PY, respectively. The NEAN cows had the latest PW at 10.8 wk. Late-calving cows had 21% lower TY210 compared to cows that calved earlier. Milk composition was influenced by cow genotype, with CRAN and NEAN cows producing milk with greater fat (3.8 and 3.9%, respectively) and protein (3.2 and 3.1%, respectively) content compared to ANAN and HHAN cows. Regardless of the genotype, fat, protein, and total solids increased in concentration from beginning to end of lactation, while lactose content decreased. Crossbreeding of Angus with adapted breeds of taurine or indicine origin can be effective in increasing milk yield and nutrient
Kammes, K L; Heemink, G B H; Albrecht, K A; Combs, D K
The mixture of kura clover (Trifolium ambiguum M. Bieb.) and reed canarygrass (Phalaris arundinacea L.) has proven to be extremely persistent in the northern United States, but information about dairy cow performance on this mixture is lacking. Twenty lactating Holstein cows were used in a crossover design to compare dry matter (DM) intake and milk production from diets containing kura clover-reed canarygrass silage (KRS) or alfalfa (Medicago sativa L.) silage (AS). Forages were cut, wilted, ensiled in horizontal plastic bags, and allowed to ferment for at least 50 d before beginning the feeding experiment. The KRS was approximately 40% kura clover and 60% reed canarygrass. Treatments were total mixed rations formulated with either 57% of total DM from 1) AS or 2) KRS. Experimental periods were 28 d, with the first 14 d for diet adaptation and the last 14 d for measurement of intake and milk production. The neutral detergent fiber (NDF) concentrations of AS and KRS were 37.3 and 47.3%, respectively. The fermentation analyses indicated that both silages underwent a restricted fermentation, producing primarily lactic acid and some acetic acid. Dry matter intake (24.2 vs. 22.8 kg) and 4% fat-corrected milk (32.8 vs. 30.9 kg) were significantly higher for cows fed AS than for cows fed KRS. Cows consumed less NDF (6.7 vs. 8.0 kg) and less digestible NDF (3.0 vs. 4.4 kg) when fed AS diets compared with KRS diets, but the pool of ruminally undegraded NDF was similar (3.7 kg) between diets. Cows produced 1.5 kg of milk/kg of DM consumed regardless of the diet, indicating that digestible NDF of KRS was utilized with similar efficiency as the cell wall constituents of AS, but the intake of cows fed KRS may have been limited by rumen fill. Milk fat concentration tended to be higher for cows fed AS, but the milk true protein concentration and yields of fat and protein did not differ by treatment. Milk urea nitrogen content was higher when cows consumed AS (16.4 mg/ d
Kiens, Bente; Richter, Erik; Wojtaszewski, Jørgen
The passing of Professor Bengt Saltin on September 12, 2014 truly marks the end of an era. As editor of the Journal of Applied Physiology and one of Bengt’s many collaborators and colleagues, I wanted the Journal to celebrate his many seminal contributions by means of an Editorial. Professor Bent...
Ovarian cortex from high A4 cows secretes excess A4, and exhibits increased oxidative stress, macrophage markers and arrested follicle development which can be partially rescued by angiogenic VEGFA Is
We have identified a population of cows within the UNL physiology herd with excess androstenedione (A4) in follicular fluid and 17% reduction in calving rate. Because excess androgens can cause follicle arrest we hypothesized that 1) folliculogenesis would be disrupted in High A4 cows; and 2) vascul...
deeper understanding on potential mechanism(s adapted by rose due to the exogenous Si supplementation during the salinity stress.
O'Toole, M L; Douglas, P S
The triathlon is a 3-event endurance sport in which athletes compete sequentially in swimming, cycling and running. The primary determinant of success is the ability to sustain a high rate of energy expenditure for prolonged periods of time. Exercise training-induced physiological adaptations in virtually all systems of the body allow the athlete to accomplish this. Aerobic capacity (measured as maximal oxygen uptake, VO2max), economy of motion (submaximal VO2) and fractional utilisation of maximal capacity (%VO2max) reflect the integrated responses of these physiological adaptations. Numerous studies have reported relatively high mean VO2max values for various groups of triathletes that are comparable to those reported for athletes in single-event endurance sports and clearly above those reported for untrained individuals. In shorter distance triathlons and in studies using recreational (rather than elite) triathletes, VO2max is related to performance in the corresponding event of the triathlon (e.g. tethered swimming VO2max with swim time). In longer events and with more elite triathletes, VO2max correlates less well with performance. The physiological adaptations that correspond to and facilitate improved VO2max occur centrally in the cardiovascular system, centred on increased maximal cardiac output, and peripherally in the metabolic systems, centred around increased arterio-venous O2 (a-v O2) difference. While a high VO2max in individuals is clearly of importance to triathlon performance, energy output must be sustained for long periods of time, making economy of motion also very important. Studies suggests that competitive swimmers have better swimming economy than triathletes. However, since many triathletes have previously been competitive swimmers this finding is questionable. The finding suggests that triathletes from nonswimming backgrounds would benefit from improving swimming technique rather than concentrating training workouts solely on distance. In
is primarily caused by dryness of the skin and is linked to hereditary factors. However ... in Table I. FPI disorders are typically cow's milk and soya protein induced3 but may also ... fish, chicken, turkey, corn and vegetables. FPI enteropathy ...
The dairy cow's teat is the first line of defence against mastitis pathogens. The milking process may affect the teat's condition, increasing the risk of mastitis. It is well-proven that teat-ends with severe erosions or broken skin will have an increased risk of mastitis. However, more common
The dairy cow's teat is the first line of defence against mastitis pathogens. The milking process may affect the teat's condition, increasing the risk of mastitis. It is well-proven that teat-ends with severe erosions or broken skin will have an increased risk of mastitis. However, more common
Full Text Available Tapetum lucidum is the specially modifided, bio-reflecting tissue layer, which at most vertebrates covers the biggest part of the middle eye tunic, the chorioidea. Physiologically, the most important role of this layer is to reflect light which passes through the retina back to it, causing the secondary stimulation of the photoreceptors. In the dim light ambient conditions when the pupil is dilated, reflected beams of the light create the visual effect of the “animals' eyes shining in the dark“, while exiting the eyes. The aims of this study are to define bio-reflecting and morphological charachteristics of the tapetum lucidum in the dog and cow eyes. Because the tapetum lucidum is an adaptive structure of the eye on poorly illuminated environment, the results of our research indicate that the anatomy of the eye, particulary shape of the pupil, placement of the eyes on the animals' head, and diet, all affect the morphology and bio-reflecting characteristics of this structure.Key words: tapetum lucidum, eyeshine, morphology
... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW equipment: Removal. 157.170... Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.170 COW equipment: Removal. (a) Whenever a deck mounted COW machine is removed from the tank, the master shall ensure that: (1) The supply...
... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW operations: Changed... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.158 COW operations: Changed characteristics. The COW system may be operated with characteristics that do not meet those...
Cavestany, D.; Tagle, R.; Lanzzeri, S.; Miranda, W.; Gama, S.
Trials to determine the onset of post-partum (pp) ovarian activity in a Holstein herd were carried out in 1985 and repeated during the same time of the year in 1987. In each, 20 recently calved adult cows and first-calf heifers were randomly selected and sampled for milk progesterone (MP 4 ) determination twice a week between days 10 and 90 pp. Climatic conditions and availability of high quality fodder were better in 1987 than 1985. Results showed differences both in pregnancy rate (40% vs. 65%), as well as in the percentage of anoestrous cows (60% vs. 15%) between 1985 and 1987, respectively. In the second trial, not all cows in physiological oestrus (according to MP 4 measurements) were identified in heat by observation of behavioural signs. It was concluded that nutritional levels greatly influence the reproductive efficiency of the herd on this farm, and that poor oestrus detection was a further contributory factor. Another trial conducted on a dairy herd in order to compare the efficiency of oestrus synchronization by PGF 2α with manual enucleation of the corpus luteum (CL) showed no differences between treatment groups. Ovarian palpation to assess the presence of a CL was accurate in 69% of cases while the use of MP 4 estimations proved to be 100% efficent. (author). 13 refs, 1 fig., 2 tabs
Summaries of research projects conducted during 1978 and 1979 are presented. Subject areas include: the effects of environmental pollutants on homeostasis of the hematopoietic system; pollutant effects on steroid metabolism; pollutant effects on pulmonary macrophages; effects of toxic gases on lung cells; the development of immunological methods for assessing lung damage at the cellular level; the response of erythropoietin concentration to various physiological changes; and the study of actinide metabolism in monkey skeletons
Associação da palma forrageira com diferentes tipos de volumosos em dietas para vacas em lactação: comportamento ingestivo e parâmetros fisiológicos - DOI: 10.4025/actascianimsci.v29i2.239 Association of the forage cactus with different types of forage in diets for lactating cows: ingestive behaviour and physiologic parameters - DOI: 10.4025/actascianimsci.v29i2.239
Marcilio de Azevedo
Full Text Available O experimento foi realizado de janeiro a abril de 2005, objetivando avaliar o comportamento ingestivo e os parâmetros fisiológicos de vacas da raça Holandesa em lactação, alimentadas com concentrado e palma forrageira associada a diferentes volumosos (bagaço de cana-de-açúcar em in natura, feno de capim-tifton, feno de capim-elefante, silagem de sorgo e bagaço de cana mais silagem de sorgo. Foram utilizadas cinco vacas, distribuídas em quadrado latino 5 x 5, após período pré-experimental de quatorze dias. Cada período experimental teve duração de 14 dias. O registro das variáveis comportamentais foi de forma visual, em intervalos de cinco minutos, em 24 horas. O ambiente foi monitorado de hora em hora, das 6h às 18h, por meio dos termômetros de bulbo seco e úmido. A temperatura retal e freqüência respiratória foram registradas às 9h e 18h. O consumo de água foi mensurado às 5h30min e 17h30min. Não houve diferença (p > 0,05 entre os volumosos, quanto às variáveis fisiológicas e comportamentais, ao número de defecação e micção e a procura por água. Houve diferença (p The experiment was carried out from January to April 2005, evaluating the ingestive behaviour and physiologic parameters of lactating Holstein cows fed with cactus forage associated with different forage (sugarcane bagasse in natura, tifton grass hay, elephant grass hay, sorghum silage and sorghum silage plus sugarcane bagasse. Five cows were assigned to a 5 x 5 latin square design, after pre-experimental period of 14 days. Each experimental period lasted 14 days. The registration of the behavior variables was in a visual way, at five-minute intervals, in 24 hours. The ambient temperature was monitored every hour, from 6 am to 6 pm, through bulb dry and humid thermometer. Rectal temperature and respiratory frequency were registered at 9 am and at 6 pm. The water intake was measured at 5.30 am and at 5.30 pm. There was no difference (p > 0.05 among
Full Text Available For the correction of reproductive function of cows with ovarian hypofunction practices use a number of hormones. Recently, to stimulate reproductive function using herbal medicines that have gonadotropic effect or stimulate secretion of steroid hormones who try to use to increase fertility. Therefore, we carried out an attempt to develop a method of regulation of reproductive function of the ovaries of cows using combination therapies that can provide effective treatment by studying the biochemical parameters of animals. The cows were divided depending on the treatment to control and two experimental groups of 5 animals in each group. Groups were formed by the following treatment regimens indicated pathology. Cows control group treated by next scheme: day 1 — intramuscular injection drug in vitro at a dose of 10 ml; day 2 —PMSG intramuscular administration of the drug at a dose of 500 IU; day 3 —intramuscular injection drug Surfahon at a dose of 50 mg. Cows from experimental group 1 was injected intramuscularly liposomal drug based on herbal (Rhodiola rosea, Salvia; Animals from second experimental group were injected intramuscularly liposomal drug based on phyto-substances (Rhodiola rosea, Salvia with gonadotropin-releasing hormone (Surfahon. Analysis of biochemical parameters of blood serum of cows with ovarian hypofunction found low concentrations of estradiol-17-β and progesterone. Between the control and experimental groups concentration of progesterone and estradiol-17-β differ within 10%, which indicates the same level of disease in all animals selected. Level carotene, ascorbic acid and cholesterol in all groups was within the physiological norm and differed slightly. It was established that the treatment of cows with hypofunction ovaries in the experimental group 1 progesterone level 7 days after treatment was 11.5, and 2 - on 41,4% (p <0,01 higher than in the control group animals, indicating that the revitalization of the
Lassen, Jan; Garnsworthy, P C; Chagunda, M G
Over the last 5 years a number of methods have been developed and adapted to measure and quantify methane emission from individual animals. Their approaches show similarities and yet also differences. In general they focus on being noninvasive measuring in the cow everyday environment. In order...
Loss, G.; Depner, M.; Ulfman, L.H.; Neerven, van R.J.J.; Hose, A.J.; Genuneit, J.
Background: Breast-feeding is protective against respiratory infections in early life. Given the co-evolutionary adaptations of humans and cattle, bovine milk might exert similar anti-infective effects in human infants. Objective: To study effects of consumption of raw and processed cow's milk on
Full Text Available This paper describes paratuberculosis in an isolated breeding herd of 25 high-yield dairy cows of the Red Holstein breed. The animals were examined clinically and then given the test for ldelayed type hypersensitivity and their blood serum was examined for the presence of specific antibodies against Mycobacterium avium subsp. paratuberculosis (Map. The clinical examination revealed that two cows exhibited symptoms of the disease that indicated an advanced stage of paratuberculosis. The following parameters were examined in the blood of the cows that showed clinical signs of the disease: leukocytes and erythrocytes count, concentrations of total proteins, albumin, iron, sodium, potassium, and activity of creatine kinase. The analysis of the red blood cell count revealed certain digressions that indicated the existence of hypochromic microcytic anaemia. The number of leukocytes was within the physiological values, but the neutrophil-lymphocyte ratio was disrupted and stood at almost 1:1. The results of the biochemical analyses of the blood serum of diseased cows indicated hypoproteinaemia, hypoalbuminaemia, hypoferremia, hyposodiumaemia, hypokalemia, and increased activities of creatine kinase enzymes. A suspect reaction on the site of application of avian tuberculin was determined in two animals. Animals with clinical signs of the disease reacted negative to the test of delayed type hypersensitivity. The presence of specific antibodies against the cause of paratuberculosis was proven in four animals (16%, including two animals with clinical signs of the disease and one that had a suspect reaction on the site of application of avian tuberculin. Furthermore, one animal that died exhibited macroscopic and microscopic changes regarding the intensity and distribution of lesions, the type of cellular infiltrate, and the number of present acidresistent bacteria, and the changes were characterized as diffuse changes of multibacillary type. The cause of
Gaillard, Charlotte; Martin, O; Blavy, P
The GARUNS model is a lifetime performance model taking into account the changing physiological priorities of an animal during its life and through repeated reproduction cycles. This dynamic and stochastic model has been previously used to predict the productive and reproductive performance...... of various genotypes of cows across feeding systems. In the present paper, we used this model to predict the lifetime productive and reproductive performance of Holstein cows for different lactation durations, with the aim of determining the lifetime scenario that optimizes cows' performance defined...... by lifetime efficiency (ratio of total milk energy yield to total energy intake) and pregnancy rate. To evaluate the model, data from a 16-mo extended lactation experiment on Holstein cows were used. Generally, the model could consistently fit body weight, milk yield, and milk components of these cows...
Barletta, R V; Gandra, J R; Freitas Junior, J E; Verdurico, L C; Mingoti, R D; Bettero, V P; Benevento, B C; Vilela, F G; Rennó, F P
The aim of this study was to evaluate the effects of high levels of whole raw soya beans in the diets of lactating cows. Twelve Holstein dairy cows were used, randomized in three 4 × 4 balanced and contemporary Latin squares and fed the following diets: (i) control (C), without including whole raw soya beans; (ii) 80 g/kg in DM of whole raw soya beans (G80); (iii) 160 g/kg in DM of whole raw soya beans (G160); and (iv) 240 g/kg in DM of whole raw soya beans (G240). There was significant reduction (p beans in dairy cow diets improves the unsaturated fatty acid profile in milk, and the diets (G80 and G160) led to minor alterations in the digestive processes and animal metabolism. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.
Full Text Available Aim: The aim of this study was to determine selenium (Se concentration in the blood serum of dairy cows and to establish its influence on the level of production and reproduction traits. Materials and Methods: The study was performed on the farm located in Western Pomerania - Poland and involved 120 cows, which were selected using the analog method on the basis of their physiological state, lactation number, milk yield, age, and genotype. The following indices were analyzed in individual groups: Calving interval, gestation interval, insemination index, standstill of placenta. Se concentration in the blood serum was determined with the spectrofluorometric method. Results: The mean serum Se concentration was in cows 0.038 μg/ml. The use of Se preparations has raised fertility, which was documented statistically. Conclusion: The study revealed that the problem of Se deficiency is still present in some dairy cattle herds in Western Pomerania - Poland.
Tauck Shaun A
Full Text Available Abstract Background The physiological mechanism by which bulls stimulate resumption of ovarian cycling activity in postpartum, anovular, suckled cows after calving may involve the concurrent activation of the hypothalamic-hypophyseal-ovarian (HPO axis and hypothalamic-hypophyseal-adrenal (HPA axis. Thus, the objectives of this experiment were to determine if characteristics of temporal patterns of cortisol and luteinizing hormone (LH in postpartum, anovular, beef cows are influenced by acute exposure to bulls. The null hypotheses were that daily, temporal characteristics of cortisol and LH concentration patterns do not differ between cows exposed acutely to bulls or steers. Methods Sixteen cows were assigned randomly 67 +/- 4 (+/- SE after calving to be exposed to bulls (EB, n = 8 or steers (ES, n = 8 5 h daily for 9 d (D 0 to 8. Blood samples were collected daily from each cow via jugular catheters at 15-min intervals for 6 h from 1000 to 1600 h each day. The 5-h exposure period began 1 h after the start of the intensive bleeding period. Characteristics of cortisol and LH concentration patterns (mean, baseline, pulse frequency, pulse amplitude, and pulse duration were identified by PULSAR analyses. Results Mean cortisol concentrations decreased (P 0.10 between EB and ES cows. The decrease in mean cortisol concentrations in EB and ES cows from D 0 to D 2 was attributed to cows acclimatizing to intensive blood sampling and handling procedures. Consequently, analyses for characteristics of cortisol and LH concentration patterns included D 2 through 8 only. Cortisol mean and baseline concentrations, and pulse amplitude did not differ (P > 0.10 between EB and ES cows. However, cortisol pulse duration tended to be longer (P = 0.09 and pulse frequency was lower (P = 0.05 in EB than ES cows. LH pulse frequency was greater (P = 0.06 in EB than ES cows. All other characteristics of LH concentration patterns did not differ (P > 0.10 between EB and ES cows
Toomingas, Allan; Tornqvist, Ewa Wigaeus
In a clear and accessible presentation, Occupational Physiology focuses on important issues in the modern working world. Exploring major public health problems-such as musculoskeletal disorders and stress-this book explains connections between work, well-being, and health based on up-to-date research in the field. It provides useful methods for risk assessment and guidelines on arranging a good working life from the perspective of the working individual, the company, and society as a whole.The book focuses on common, stressful situations in different professions. Reviewing bodily demands and r
Zhou, Z; Bulgari, O; Vailati-Riboni, M; Trevisi, E; Ballou, M A; Cardoso, F C; Luchini, D N; Loor, J J
The immunometabolic status of peripartal cows is altered due to changes in liver function, inflammation, and oxidative stress. Nutritional management during this physiological state can affect the biological components of immunometabolism. The objectives of this study were to measure concentrations of biomarkers in plasma, liver tissue, and milk, and also polymorphonuclear leukocyte function to assess the immunometabolic status of cows supplemented with rumen-protected methionine (Met) or choline (CHOL). Forty-eight multiparous Holstein cows were used in a randomized complete block design with 2×2 factorial arrangement of Met (Smartamine M, Adisseo NA, Alpharetta, GA) and CHOL (ReaShure, Balchem Inc., New Hampton, NY) level (with or without). Treatments (12 cows each) were control (CON), no Met or CHOL; CON and Met (SMA); CON and CHOL (REA); and CON and Met and CHOL (MIX). From -50 to -21d before expected calving, all cows received the same diet [1.40Mcal of net energy for lactation (NE L )/kg of DM] with no Met or CHOL. From -21d to calving, cows received the same close-up diet (1.52Mcal of NE L /kg of DM) and were assigned randomly to each treatment. From calving to 30d, cows were on the same postpartal diet (1.71Mcal of NE L /kg of DM) and continued to receive the same treatments until 30d. The Met supplementation was adjusted daily at 0.08% DM of diet, and CHOL was supplemented at 60g/cow per day. Liver (-10, 7, 21, and 30d) and blood (-10, 4, 8, 20, and 30d) samples were harvested for biomarker analyses. Neutrophil and monocyte phagocytosis and oxidative burst were assessed at d 1, 4, 14, and 28d. The Met-supplemented cows tended to have greater plasma paraoxonase. Greater plasma albumin and IL-6 as well as a tendency for lower haptoglobin were detected in Met- but not CHOL-supplemented cows. Similarly, cows fed Met compared with CHOL had greater concentrations of total and reduced glutathione (a potent intracellular antioxidant) in liver tissue. Upon a
Kynchev, L; Naidenov, N
30 - 50 microlitres of milk are diluted in pH 6.9 - 7.5 phosphate buffer in the ratio of 1:3 to 1:6. Then 200 microlitres of radioactive progesterone and its antibody in the ratio 1:1 are added and incubated for 80 - 90 minutes at 2 - 7 deg. C. After the expiration of the incubation period 0.5 - 0.1 millilitres of 0.2 - 0.6 percent narcol suspension are added, followed by 10 minutes of centrifugation at 2 - 7 deg C and 2200 - 3800 rpm. The resultant fluid is then poured into vials containing 5 - 10 millilitres of scintillation liquid. Then the samples are subjected to B-scintillation count. If blood contains up to 20 picogrammes of progesterone the cow is not pregnant, if the blood contains 20 - 30 picogrammes - there are reasonable doubts, and over 30 picogrammes the cow is pregnant.
Harms, H K
Cow's milk protein sensitive enteropathy (CMPSE) is characterized by the following items: 1. The great majority of affected infants have not been breast fed or only for a few days. Additional risks are immaturity, preceding enteritis, trisomy 21, and abdominal operation in the newborn. 2. Half of the patients become ill during the first two weeks after starting cow's milk formula. The main symptoms are watery, mucus containing diarrhea, vomiting, abdominal distension, pallor and rapid weight loss. 3. In CMPSE the small intestinal mucosa shows varying degrees of inflammation and villous atrophy. Bloody stools refer to large bowel affection. 4. CMPSE is always transitory and usually persists for less than one year. Inadequate treatment leads to "severe protracted diarrhea" or "intractable diarrhea" syndrome. Soya-based formula should not be the diet of first choice, since secondary intolerance to soya proteins will frequently develop. Exclusive breast feeding during the first months of life is the best prophylaxis of CMPSE.
Full Text Available Negative energy balance can impair the metabolism of high producing dairy cows and supplying the glucose, as an energy source; can prevent the metabolic disorders in these animals. Hence, we hypothesized that bolus intravenous glucose administration may change the concentrations of metabolic hormones in order to prevent and control of metabolic dysfunctions of dairy cows. Twenty five multiparous Holstein dairy cows were divided to 5 equal groups containing early, mid and late lactations, far-off and close-up dry periods. All cows were received dextrose 50% intravenously at 500 mg/kg, 10 mL/kg/h. Blood samples were collected from all animals prior to and 1, 2, 3 and 4 after dextrose 50% infusion and sera were separated to determine glucose, triiodothyronine (T3, thyroxine (T4, serum free T3 (fT3, free T4 (fT4, cortisol and insulin like growth factor-1 (IGF-1. The decreasing pattern of T3 concentration was detected in all studied animals following intravenous glucose infusion (P<0.05. The significant increasing pattern of T4 levels was seen in early and mid lactation cows after glucose administration (P<0.05. The significant decreasing pattern of IGF-1 was detected in mid and late lactations and far-off dry groups (P<0.05. There were no significant alterations in fT3, fT4 and cortisol concentrations following glucose infusion in all experimental groups. In conclusion, bolus intravenous glucose infusion could influence the metabolic hormones in high producing Holstein dairy cows. Alterations of metabolic hormones following bolus intravenous glucose administration indicated that glucose is an important direct controller of metabolic interactions and responses in dairy cows during different physiological states.
Adaptive capability as indicated by behavioral and physiological responses, plasma HSP70 level, and PBMC HSP70 mRNA expression in Osmanabadi goats subjected to combined (heat and nutritional) stressors
Shilja, Shaji; Sejian, V.; Bagath, M.; Mech, A.; David, C. G.; Kurien, E. K.; Varma, Girish; Bhatta, Raghavendra
A study was conducted to assess the impact of heat and nutritional stress simultaneously on the adaptive capability as indicated by behavioral and physiological responses, plasma heat shock protein 70 (HSP70) level, and peripheral blood mononuclear cells (PBMC) HSP70 gene expression in goats. Twenty-four adult Osmanabadi bucks (average body weight (BW) 16.0 kg) were used in the present study. The bucks were divided into four groups viz., C ( n = 6; control), HS ( n = 6; heat stress), NS ( n = 6; nutritional stress), and CS ( n = 6; combined stress). The study was conducted for a period of 45 days. C and HS bucks had ad libitum access to their feed while NS and CS bucks were under restricted feed (30 % intake of C bucks) to induce nutritional stress. The HS and CS bucks were exposed to solar radiation for 6 h a day between 10:00 a.m. and 4:00 p.m. to induce heat stress. The data was analyzed using repeated measures analysis of variance. The standing time differed significantly ( P < 0.01) between ad libitum fed groups (C and HS) and restricted feeding groups (NS and CS). The highest ( P < 0.01) lying time was recorded in the CS group while the lowest in the C and HS groups. The highest ( P < 0.01) drinking frequency was also recorded in the CS group. Water intake recorded was significantly ( P < 0.01) higher in both the HS and CS groups. The highest respiration rate (RR), pulse rate (PR), and rectal temperature (RT) during the afternoon were also recorded in the CS group. Further, skin temperature of the head, flank, and scrotum during the afternoon was also higher ( P < 0.01) in the CS group. In addition, both plasma HSP70 concentration and PBMC HSP70 messenger RNA (mRNA) transcript expression were also significantly ( P < 0.01) higher in the CS group. It can be concluded from this study that when two stressors occur simultaneously, they may have severe impact on adaptive capabilities of Osmanabadi bucks as compared to that would occur individually. Further, the
Gionbelli, Mateus P.; Duarte, Marcio S.; Valadares Filho, Sebastião C.; Detmann, Edenio; Chizzotti, Mario L.; Rodrigues, Felipe C.; Zanetti, Diego; Gionbelli, Tathyane R. S.; Machado, Marcelo G.
Background Beef cows herd accounts for 70% of the total energy used in the beef production system. However, there are still limited studies regarding improvement of production efficiency in this category, mainly in developing countries and in tropical areas. One of the limiting factors is the difficulty to obtain reliable estimates of weight variation in mature cows. This occurs due to the interaction of weight of maternal tissues with specific physiological stages such as pregnancy. Moreover, variation in gastrointestinal contents due to feeding status in ruminant animals is a major source of error in body weight measurements. Objectives Develop approaches to estimate the individual proportion of weight from maternal tissues and from gestation in pregnant cows, adjusting for feeding status and stage of gestation. Methods and Findings Dataset of 49 multiparous non-lactating Nellore cows (32 pregnant and 17 non-pregnant) were used. To establish the relationships between the body weight, depending on the feeding status of pregnant and non-pregnant cows as a function of days of pregnancy, a set of general equations was tested, based on theoretical suppositions. We proposed the concept of pregnant compound (PREG), which represents the weight that is genuinely related to pregnancy. The PREG includes the gravid uterus minus the non-pregnant uterus plus the accretion in udder related to pregnancy. There was no accretion in udder weight up to 238 days of pregnancy. By subtracting the PREG from live weight of a pregnant cow, we obtained estimates of the weight of only maternal tissues in pregnant cows. Non-linear functions were adjusted to estimate the relationship between fasted, non-fasted and empty body weight, for pregnant and non-pregnant cows. Conclusions Our results allow for estimating the actual live weight of pregnant cows and their body constituents, and subsequent comparison as a function of days of gestation and feeding status. PMID:25793770
The Khadi and Village Industries Commission offers a gobar gas (methane gas) production scheme. The gas plant, available in sizes of 60 to 3000 cu ft, requires only low maintenance expenditures. The cow dung, which is at present being wasted or burned as domestic fuel, can be used for manufacturing methane for fuel gas. The residue will be a good fertilizer for increasing food production. There are now about 4000 gobar gas plants in India.
Perri, A F; Mejía, M E; Licoff, N; Lazaro, L; Miglierina, M; Ornstein, A; Becu-Villalobos, D; Lacau-Mengido, I M
Parasitism in cattle is known to impair growth and development. Recent findings suggest that productivity of adult animals is also affected, but little is known about the physiological mechanisms involved. Furthermore, development of nematode resistance to drugs makes imperative the search of management practices that avoid whole herd treatment. We undertook an epidemiological and endocrine study in a grass based dairy farm in Argentina to study the effect of parasites on milk production and the underlying mechanisms involved, and identify individual animals that would benefit from antiparasitic treatment. All the cows in the dairy were followed monthly for egg parasite output in feces. Samples were cultured for genera determination. Milk production and reproductive results were recorded and periodical bleedings for hormone determination were performed. Nematode egg output (EPG) was maximal in late Summer and Autumn and minimal in Spring in coincidence with the Ostertagia inhibition-disinhibition cycle as this genus had the highest prevalence in all the study. The highest proportion of positive samples was found in the high producing herd and maximal counts were found in the peripartal period. Milk production did not correlate with EPG mean values but, when cows were grouped by EPG positivity around parturition, a significant difference in total milk production between EPG null and positive cows was observed. Positive cows produced 7%, 12% or 15% less milk than null EPG cows, depending on the sampling month/s chosen for classification. The highest difference was seen when both prepartum and postpartum samples were taken into account. No difference in lactation length and a marginal effect on partum to first service interval were encountered. Endocrine studies revealed a decrease in serum growth hormone (GH), type I insulin-like growth factor (IGF-I) and prolactin during lactation in cows with positive EPG in the first postpartum sample with respect to null EPG cows
Frey, H-J; Gross, J J; Petermann, R; Probst, S; Bruckmaier, R M; Hofstetter, P
Feeding dairy cows indoors or on pasture affects not only labour, machinery and housing costs, but also animals' performance and metabolism. This study investigates the effects of indoor feeding (IF) with a partial-mixed ration (PMR) versus pasture-based feeding (PF) on milk production, fertility, backfat thickness (BFT), body weight (BW) loss and energy metabolism of Brown Swiss (BS) dairy cows with similar genetic production potential. The IF herd consisted of 13 cows fed a PMR composed of maize and grass silage plus protein concentrate according to each cow's requirements. The PF herd consisted of 14 cows offered barn-ventilated hay ad libitum after calving from January until March and grazed on semi-continuous pastures during the vegetation period. The IF cows produced more energy-corrected milk (ECM) per standard lactation (9,407 vs. 5,960 kg; p dairy cows in our trial seem to have a high capacity for metabolic adaptation to different production systems. © 2017 Blackwell Verlag GmbH.
Gonçalves,Geógenes da Silva; Pedreira,Marcio dos Santos; Pereira,Mara Lúcia Albuquerque; Santos,Dimas Oliveira; Souza,Dicastro Dias de; Porto Junior,Antonio Ferraz
The aim of this study was to evaluate the effect of dairy cow diets containing two different sources of urea on nitrogen metabolism and microbial synthesis. Eight crossbred cows were confined and distributed in two 4x4 Latin: FS - soybean meal (control), conventional urea (UC) 100%; ULL 44 UC = 56% / 44% ULL, ULL 88 UC = 12% / 88% ULL. Diets were offered to animals during 21 days with 14 days of adaptation. The N intake, retained nitrogen balance, nitrogen excretion in milk, urine, feces and ...
Gleidiana Amélia Pontes de Almeida; Marcelo de Andrade Ferreira; Janaina de Lima Silva; Juana Catarina Cariri Chagas; Antônia Sherlânea Chaves Véras; Leonardo José Assis de Barros; Gledson Luiz Pontes de Almeida
Objective The study aimed to evaluate sugarcane bagasse as roughage in lactating cow on feed intake, digestibility, ingestive behavior, milk production and composition, and microbial protein synthesis. Methods Ten Girolando cows at initial body weight of 450±25.6 kg and at 143.7±30.7 days in milk were assigned in two 5×5 Latin square designs. Five 21-day experimental periods were adopted (1° to 14-day: diets adaptation period; 15° to 21-day: data collection and sampling period). The diets con...
Mendes, Aline Medeiros de Paula; Silva, Dulciene Karla de Andrade; Ferreira, Marcelo de Andrade; Veras, Antonia Sherlânea Chaves; Ferreira, Geane Dias Gonçalves; Vigoderis, Ricardo Brauer; Arcanjo, Helton Gregory Santos; Silva, Jarbas Miguel; de Almeida, Josimar Santos; Fotius, Anna Christine Alencar; Farias, Glébio de Almeida
The aim of this study was to assess the effect of different supplements on the behavior variables of crossbred dairy cows that were in pasture that comprised two 4 × 4 Latin squares (four periods, four treatments, and four animals). Each experimental period lasted 15 days (10 days to adapt animals to treatments and 5 days for data collection). The animals were supplemented twice a day, with different forage (corn silage and cactus pear) and concentrate sources (soy mea + wheat meal + corn meal + cotton seed together and soy meal as a single constituent of the concentrate). A significant difference (p ingestive behavior of crossbred dairy cows.
Young, Eric D.
The analysis of physiological sound in the peripheral auditory system solves three important problems. First, sound energy impinging on the head must be captured and presented to the transduction apparatus in the ear as a suitable mechanical signal; second, this mechanical signal needs to be transduced into a neural representation that can be used by the brain; third, the resulting neural representation needs to be analyzed by central neurons to extract information useful to the animal. This chapter provides an overview of some aspects of the first two of these processes. The description is entirely focused on the mammalian auditory system, primarily on human hearing and on the hearing of a few commonly used laboratory animals (mainly rodents and carnivores). Useful summaries of non-mammalian hearing are available . Because of the large size of the literature, review papers are referenced wherever possible.
Full Text Available It is considered that high air temperature and humidity during the summer are the main factors which adversely affect both the health and production-reproductive performance of high yielding dairy cows. The resulting heath stress leads to a series of changes in endocrine regulation of homeostasis. The changes in hormonal status reflect in some way to the indicators of metabolic status of the cows. The objective of this work was to investigate the influence of heat stress on metabolic status of cows. The experiment was carried out on 20 cows of Holstein-Friesian breed during the summer, in the period from 18th to 45th day of lactation. During the performance of the experiment, the value of heat index (THI was determined hourly and then the value of average morning (from 10 pm the previous day to 9 am the current day, afternoon (from 10 am to 9 pm the current day and all-day THI was calculated. Blood sampling was carried out on the 1st, 2nd, 8th, 11th, 14th, 18th, 25th, 29th and 37th day of the experiment, in the morning and the afternoon. On the basis of hourly THI values, whole experimental period was divided into three periods: period A during which the cows were exposed to a extreme high heat stress (THI≥78 at least 7 hours in 24 hours; period B during which the cows were exposed to a moderate heat stress (72≥THI≤78 at least 7 hours in 24 hours; period C during which the cows were not exposed to a heat stress (THI≤72 in 24 hours. The average daily THI in period A (73,25±0,89 was significantly higher (p<0,01, individually in regard to period B (71,45±0,96 and period C (65,41±2,09. THI was significantly higher in the period B than in the period C (p<0,01. Significantly lower blood glucose value (p<0,05 during the afternoon period in the cows exposed to the extreme heat stress (3,02±0,31 mmol/L in regard to the morning period (3,14±0,41 mmol/L points to the fact that in such conditions, metabolism redirects to use of glucose as an
Full Text Available The aim of this study was to examine the characteristics of the metabolic profile and complete blood count in cows in the periparturient period on the basis of the intensity of lipolysis and ketogenesis (concentration of non esterified fatty acid - NEFA and betahydroxybutyrate - BHB. Based on median values of NEFA and BHB cows were divided into 3 groups: cows physiologically burdened with catabolism (NEFA and BHB levels above the median one week after parturition, cows significantly burdened with catabolism (NEFA and BHB levels above the median one week before and after parturition and cows that are not burdened with catabolism (NEFA and BHB below the median, i.e. the control group. The median value of NEFA was 0.27 mmol/L one week before parturition and 0.61 mmol/L one week after it. The median value of BHB was 0.51 mmol/L one week before parturition and 0.99 mmol/L one week after it. A significant group effect was shown for each week separately, so that cows physiologically burdened with catabolism and/or cows significantly burdened with catabolism compared to the control group have the following features of the metabolic profile and complete blood count: higher concentrations of NEFA and BHB (weeks: -1, 1, 2, 4, 8, lower concentrations of glucose (weeks: 1, 4, lower concentration of cholesterol (week 8, lower concentrations of total protein (weeks: 1, 2, lower concentrations of urea (weeks: 1, 2, 4, 8 and a higher concentration of bilirubin (weeks: - 1, 1, 2, 4, 8, increased levels of AST (weeks: -1, 1 and ALT (weeks: -1, 2, lower value of Ca (week -1, lower hemoglobin concentration (week -1, lower white blood cell count (week 4, a larger number of neutrophils (weeks: -1, 1, 2 and a higher number of lymphocytes (week 4. Using the method of factor analysis and principal components showed that NEFA, BHB and glucose are the major components that affect the metabolic profile and blood count, making 71.8% of the variability of all parameters. Cows
Bijl, E.; Vries, de R.F.M.; Valenberg, van H.J.F.; Huppertz, T.; Hooijdonk, van A.C.M.
The average casein micelle size varies widely between milk samples of individual cows. The factors that cause this variation in size are not known but could provide more insight into casein micelle structure and into the physiology of casein micelle formation. The objective of this research was
Nielsen, Nicolaj Ingemann; Hameleers, A; Young, F J
The present experiment examined the effect of offering either a high- (H) or low- (L) energy-density diet in late gestation and early lactation on physiological parameters, body condition score (BCS) and milk production in early lactation. In all, 40 multiparous Holstein cows were randomly alloca...
Lange, K. A.
Research in the field of animal and human physiology is reviewed. The following topics on problems of physiological science and related fields of knowledge are discussed: neurophysiology and higher nervous activity, physiology of sensory systems, physiology of visceral systems, evolutionary and ecological physiology, physiological cybernetics, computer application in physiology, information support of physiological research, history and theory of development of physiology. Also discussed were: artificial intelligence, physiological problems of reflex therapy, correlation of structure and function of the brain, adaptation and activity, microcirculation, and physiological studies in nerve and mental diseases.
... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Cow Bayou. 117.965 Section 117.965 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Texas § 117.965 Cow Bayou. The draws of the Orange County...
Scherpenzeel, C G M; Hogeveen, H; Maas, L; Lam, T J G M
The objective of this study was to develop a mathematical model to identify a scenario with the lowest costs for mastitis associated with the dry period while restricting the percentage of cows to be dried off with dry cow antimicrobials. Costs of clinical and subclinical mastitis as well as
Jakusova, Lubica; Jesenak, Milos; Schudichova, Jela; Banovcin, Peter
Children with cow milk allergy are suspected to develop calcium metabolism disturbances. We observed increased markers of bone turnover in these children. Children with cow milk allergy are more prone to develop the disturbances of the bone mineralization even in the first year of life.
Piechotta, M; Sander, A K; Kastelic, J P; Wilde, R; Heppelmann, M; Rudolphi, B; Schuberth, H J; Bollwein, H; Kaske, M
Because peripartal production diseases are prevalent in dairy cows, early recognition is crucial. Several studies reported metabolic variables as risk predictors for subsequent diseases. To improve on-farm testing and application of those methods, the sampling procedure should take into account variation in gestation length. Furthermore, additional variables indicating cows at risk of any production disease should be sought. Therefore, the objective was to characterize differences between cows with and without postpartum production disease (retained fetal membranes, ketosis, hypocalcemia, abomasal displacement, metritis, mastitis) by prepartum measurement of serum nonesterified fatty acid (NEFA) and plasma insulin-like growth factor (IGF)-I concentrations relative to the artificial insemination (AI) that established pregnancy. Blood was collected from 41 Holstein Friesian cows on 235 to 241, 242 to 248, 249 to 255, 256 to 262, 263 to 269, 270 to 276, 277 to 283, and 284 to 290 d after AI. Health status was assessed daily for 3 wk after calving; 25 cows (66%) had at least one production disease. Cows developing postpartum diseases had higher mean serum NEFA concentrations (450 ± 26 μmol/L; mean ± SE) and lower plasma IGF-I concentrations (78 ± 6 ng/mL) prepartum compared with healthy cows (259 ± 19 μmol/L and 117 ± 8 ng/mL, respectively). In conclusion, because of substantial variation among cows in gestation length, blood samples should be collected and studies performed on risk prediction relative to AI rather than expected date of calving. As the somatotropic axis is one of the key regulators of metabolic adaption for onset of lactation, IGF-I might be a useful variable to differentiate between cows susceptible to production diseases and cows that are able to adapt adequately within the transition period and remain healthy. Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Daniella Flavia Vilas Boas
Full Text Available For dairy cattle breeds, mainly the taurine ones, the selection emphasized for many years the increase in milk yields and, as a consequence, the adaptive and reproductive traits were negatively affected. The aim of this study was to verify the influence of genetic and environmental effects on the reproductive traits in a dairy herd selected for high milk production levels. The data set comprised 1,737 first lactations Holsteins cows of Agrindus Farm, located at Southeastern region of Brazil. The records of the following reproductive traits: calving to first heat interval (CFHI, calving to conception interval (CCI and first to second calving interval (FCI were analyzed as dependent variables by least squares method using GLM procedure (SAS. Linear models were considered including two production levels (1= less than 9,500 kg and 2= more than 9,501 kg of total milk yield, contemporary group (year and months calving, management group, sire of cow, and the sire used to breeding cows, as classificatory variables. As covariates were included for all traits the peak milk yield in lactation (linear effect, age at calving only for CFHI (linear and quadratic effects since this effect was not significant for other traits, and CFHI (linear effect only for FCI. The coefficients of determination represented 24%, 74% and 75%, respectively for CFHI, FCI and CCI models. Production level, peak milk yield and sire effects were significant (P<0.05 for all traits. The average estimated for high and low milk production level were 73 and 79 days, 500 and 601 days, 227 and 330 days for CFHI, FCI and CCI, respectively, suggesting that cows with higher genetic potential for milk had worse reproductive performance. Similarly, lactation peak showed significant effect (P<0.05 for all traits, suggesting higher peaks cows showed also poorer reproductive rates. Sire effect also was a variable that showed significant effect (P<0.01 for all traits, which means that there was
Luciano dos Santos Lima
Full Text Available The objective of this study was to determine the optimal level of licuri oil to use in the concentrate of lactating dairy cows on pasture, through growth performance, feed conversion and cost of the supplementation. A total of 16 dairy cows, Holstein × Zebu crossbreed, were kept on Tanzania grass pasture. Cows were divided into four Latin squares, 4 × 4, formed by four experimental periods of 21 days, divided into 17 days for adaptation and four days for data collection. Cows received three kg of concentrate per day at the time of milking, and the treatments consisted of four diets containing licuri oil at levels of 0.0, 1.5, 3.0, and 4.5% of the concentrated dry matter. There was a linear increase in the daily milk yield, corrected to 3.5% fat, and an improvement in the feed conversion of the dry matter and neutral detergent fiber with the inclusion of the licuri oil. The optimal level of licuri oil was 1.5% of the concentrated DM for dairy cows on pasture, whose level has the best profit sale of milk, with positive results in the corrected daily milk production and conversion of the feed nutrients.
Alireza Heravi Mosavi
Full Text Available Introduction Secondary sex ratio (SSR is the proportion of males to females at birth. It has been shown in many different mammalian species, many factors are associated with SSR. Changes in secondary sex ratio in dairy cows is considered economically important and the ability to change it could affect the revenues and profitability of a dairy farm. Thus, sperm or embryo sexing techniques in recent years has attracted more attention. Most breed of dairy cattle are more likely to have female calf is born to use them as replacement heifers and in order to maintain their productive herd number. On the contrary, when the goal is the production of meat, bull calves due to higher growth rates and production efficiency, are more convenient and more economically efficient. The aim of present study was to investigate some key factors affecting SSR in Iranian Holstein cows. According to Fisher, the sex ratio in the population under the control of natural selection is not always the same. There is overwhelming evidence to support the theory that shows Fisher Primary and secondary sex ratio sex ratio can deviate from this balance and natural selection caused a change in this ratio can be in certain circumstances. For example, the secondary sex ratio of 52:48 has been reported in dairy cows. Studies on mammalian species suggest that several factors, including latitude of the location, the dominant regional climate model, time and frequency of mating to ovulation, diet, age of parents, physical score, breed and produced eggs from ovarian left or right can have a significant effect on the secondary sex ratio. Weather conditions may modify the internal environment and the effect on physiological mechanisms or through the impact on the frequency and type of foods available to parents, the secondary sex ratio is impressive. The impact on the quantity and quality of parent's access to food sources in many species of mammals, the sex ratio has been fixed. Previous
Rudyk, Valentine Yu.; Tereshchenko, Mykola F.; Rudyk, Tatiana A.
Practical realization of adaptive control in magnetotherapy apparatus acquires an actual importance on the modern stage of development of magnetotherapy.The structural scheme of method of adaptive impulsive magnetotherapy and algorithm of adaptive control of feed-back signal during procedure of magnetotherapy is represented.A feed-back in magnetotherapy complex will be realized with control of magnetic induction and analysis of man's physiological indexes (temperature, pulse, blood prassure, ...
Nyman, A-K; Persson Waller, K; Bennedsgaard, T W; Larsen, T; Emanuelson, U
The objective of this study was to investigate if and how cow factors and intramammary infection (IMI) are associated with 4 different udder-health indicators in dairy cows as a first step in investigating whether the diagnostic performance of these indicators can be improved. The investigated indicators were somatic cell count (SCC), lactate dehydrogenase (LDH), N-acetyl-β-d-glucosaminidase (NAGase), and alkaline phosphatase (AP) measured in milk. In this cross-sectional study, approximately 1,000 cows from 25 dairy herds were sampled for bacteriology (quarter milk samples) during 3 consecutive days: the day before test milking, at the day of test milking, and at the day after test milking. The whole-udder test milking sample was analyzed for milk composition, SCC, LDH, NAGase, and AP. Cow data (parity, breed, milk yield, percentage of milk fat and protein, milk urea concentration, and days in milk from the sampled test milking) were collected from the Swedish milk-recording scheme. Of the sampled cows 485 were considered IMI negative and were used in multivariable mixed-effect linear regression models to investigate associations between cow factors and the udder-health indicators. A second modeling including all cows, both IMI negative and IMI positive (256 cows), was also performed. The results showed that all udder-health indicators were affected by cow factors but that different cow factors were associated with different indicators. Intramammary-infection status was significantly associated with all udder-health indicators except AP. Parity and milk urea concentration were the only cow factors associated with all indicators in all models. The significant cow factors explained 23% of the variation in SCC and >30% of the variation in LDH, NAGase, and AP in IMI-negative cows, showing that LDH, NAGase, and AP are more affected than SCC by cow factors. The IMI status explained 23% of the variation in SCC in the model with all cows but only 7% of the variation in
Full Text Available The impact of two years (2002 and 2003 with different summer temperature extremes on variation in metabolic profile was analyzed in blood and urine samples taken from healthy, primiparous (n = 371 and multiparous (n = 795 high yielding Holstein-Friesian dairy cows. In this study main focus was lead on three most critical physiological phases, thus cows were assigned into three groups as follows: (1 dry cows for 10 days prior to calving; (2 cows 1-30 days after delivery, and (3 cows with more than 31 days post partum. Findings reveal clear response of the cows to heat in selected blood (hemoglobin, plasma aceto-acetic-acid, FFA, AST, glucose, urea and urine (pH, NABE and urea parameters. In the majority of cows, glucose and hemoglobin level, one of the most significant blood parameters, indicated symptoms of insufficient energy supply. Further metabolic indicators differed more or less from reference values depending on actual condition. Due to heat load dry matter intake has been decreased even by 10-15 per cent in primiparous cows. They were expected to increase body weight and size and simultaneously produce attain at large milk yields. In doing so that cows would have require large amount of nutrients. Out of parameters such as hemoglobin, glucose, FFA, AST and blood-urea differed from the reference values in most cases; however, this phenomenon seemed to be present in almost every case for hemoglobin and glucose. The lack of energy caused by heat stress can be contributed to the decrease of dry matter intake which has been indicated by the urea levels and pH both in blood and urine prevailing unfavorable and insufficient feeding practice. The results reconfirm the need to reconsider both the actual feeding practice (e.g. to increase of nutrient content in rations, reduce the intake of soluble proteins in rumen, pay attention of crude fiber in Total Mixed Rations (TMR, NDF and ADF, avoid overfeeding of inorganic buffers, to control moisture
Crookenden, M A; Walker, C G; Peiris, H; Koh, Y; Heiser, A; Loor, J J; Moyes, K M; Murray, A; Dukkipati, V S R; Kay, J K; Meier, S; Roche, J R; Mitchell, M D
Biomarkers that identify prepathological disease could enhance preventive management, improve animal health and productivity, and reduce costs. Circulating extracellular vesicles, particularly exosomes, are considered to be long-distance, intercellular communication systems in human medicine. Exosomes provide tissue-specific messages of functional state and can alter the cellular activity of recipient tissues through their protein and microRNA content. We hypothesized that exosomes circulating in the blood of cows during early lactation would contain proteins representative of the metabolic state of important tissues, such as liver, which play integral roles in regulating the physiology of cows postpartum. From a total of 150 cows of known metabolic phenotype, 10 cows were selected with high (n=5; high risk) and low (n=5; low risk) concentrations of nonesterified fatty acids, β-hydroxybutyrate, and liver triacylglycerol during wk 1 and 2 after calving. Exosomes were extracted from blood on the day of calving (d 0) and postcalving at wk 1 and wk 4, and their protein composition was determined by mass spectroscopy. Extracellular vesicle protein concentration and the number of exosome vesicles were not affected by risk category; however, the exosome protein cargo differed between the groups, with proteins at each time point identified as being unique to the high- and low-risk groups. The proteins α-2 macroglobulin, fibrinogen, and oncoprotein-induced transcript 3 were unique to the high-risk cows on d 0 and have been associated with metabolic syndrome and liver function in humans. Their presence may indicate a more severe inflammatory state and a greater degree of liver dysfunction in the high-risk cows than in the low-risk cows, consistent with the high-risk cows' greater plasma β-hydroxybutyrate and liver triacylglycerol concentrations. The commonly shared proteins and those unique to the low-risk category indicate a role for exosomes in immune function. The data
Jensen, Margit Bak
. Within minutes after calving cows stood up and licked their calves; second parity cows had a longer latency to stand than later parity cows. Sniffing and licking the calf peaked during the first hour after calving and decreased during the five successive hours, while calves’ sniffing the cow peaked...
... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW tank washing machines. 157... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.124 COW tank washing machines. (a) COW machines must be permanently mounted in each cargo tank. (b...
... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW system: Evidence for... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Inspections § 157.148 COW system... inspector evidence that the COW system has been installed in accordance with the plans accepted under § 157...
The general aim of the present work was to investigate phosphorus balance in the dairy cow, with reference to the amount and source of phosphorus. Furthermore, biochemical bone markers were used to study the bone turnover during the lactation and dry period. Phosphorus is located in every cell of the body and has more known functions than any other mineral element in the animal body. Phosphorus is also an important constituent of milk, and is therefore required in large amounts in a high yiel...
This report documents the significant upgrades and improvements made to the correlator optical wavefront sensor (COWS) optical bench during this phase of the program. Software for the experiment was reviewed and documented. Flowcharts showing the program flow are included as well as documentation for programs which were written to calculate and display Zernike polynomials. The system was calibrated and aligned and a series of experiments to determine the optimum settings for the input and output MOSLM polarizers were conducted. In addition, design of a simple aberration generation is included.
Dam, K.G. van
It appears that the physiological and biochemical adaptation of skeletal muscle to training in equine species shows a lot of similarities with human and rodent physiological adaptation. On the other hand it is becoming increasingly clear that intra-cellular mechanisms of adaptation (substrate
Kidane, A; Nesheim, I L; Larsen, H J S
The objective of the current experiment was to compare the effects of supplementing mid-lactation dairy cows with all-rac-α-tocopheryl acetate (SyntvE), RRR-α-tocopheryl acetate (NatvE) or seaweed meal (Seaweed) in the presence of a Control group (no supplemental vitamin E or seaweed......) on the concentration of α-tocopherol in plasma and milk, and antibody response following immunization. The hypothesis was that supplementation of dairy cows with vitamin E, regardless of its form, would increase plasma and milk α-tocopherol compared to the control diet and this incremental response would be bigger...... with NatvE than SyntvE. Furthermore, it was hypothesized that vitamin E, regardless of its form, will provide an improved adaptive immune response to immunization than the Control diet, and cows supplemented with Seaweed meal would produce better adaptive immune response following immunization than cows...
Full Text Available The general aim of the present work was to compare dairy performance and some reproductive parameters of Holstein-Friesian (HF cows imported from Sweden as an in-calf heifers, and their age mates in Poland. The animals were kept freely on the deep litter on the farm owned by Agro-company “Witkowo” and feeding was based on TMR (total mixed ration system during whole year. All ration’s ingredients (bulky feed, concentrate, miscellaneous additives were mixed and fed as all-mash. The feed ration depended from the physiological condition of a cow and it was composed of corn and grass ensilage, mash concentrate, crushed corn meal, brewer’s grains and beet pulp.Cows imported from Sweden as in-calf heifers obtained higher milk, fat and protein yield in both lactations comparing to home cows. Significant statistical differences were observed for kg of milk, kg of protein, for FCM (P<0.01 and for kg of fat (P<0.05 in 2nd lactation. Fat and protein content in milk was in average higher for home cows (P<0.01. Big differences (P<0.01 were observed in SBT (proportion of protein to fat content and RTB (difference between concentration of fat and protein at imported cows in 2nd lactation. As far as reproduction parameters (gestation interval, calving interval, insemination index are concerned was cows from Sweden better too. Heifers from Sweden calved earlier. The usage of high genetic potential of imported cows is possible only with providing them optimum living conditions suitable to their needs.
Nielsen, B.H.; Jacobsen, S.; Andersen, P.H.
The concentrations of the two acute phase proteins, serum amyloid A and haptoglobin, in serum and milk were compared in 10 cows with clinical mastitis, 11 cows with extramammary inflammatory conditions and 10 clinically healthy control cows. The concentrations of both acute phase proteins were...... higher in the serum and milk of the cows with mastitis than in the cows in the other two groups. Four of the cows with extramammary inflammatory conditions had serum amyloid A concentrations in serum above 100 mug/ml, but negligible concentrations in milk, indicating that a pathogen must be present...
... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW operations: General. 157.155... Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.155 COW operations: General. (a) The master of a tank vessel having a COW system under § 157.10(e), § 157.10a(a)(2), or 157.10c(b)(2) shall...
... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW operations: Meeting manual... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.156 COW operations... COW system under §§ 157.10(e), 157.10a(a)(2), or 157.10c(b)(2) that has the Crude Oil Washing...
Full Text Available Weight of cows affects a large number of factors. Regular weighing and data processing can detect differences that may indicate disorders requiring nursing interventions, e.g. nutritional deficiencies, incorrect fetal development and health problems. The current weighing systems operate as stationary - the animal is fixed, identified and weighed. However, the procedure is time consuming and operation, and that is way this system is used minimally. That implies the need of complete automation of all activities associated with the weighing, which enables introduction of pass – through weight. The aim of this thesis was to develop a methodology for evaluating health and nutritional status of the herd based on data from an automated system for weighing a live weight of dairy cows. There was used in the weighing unit for milking robots Astronaut A3 (Lely company to obtain weight data of individual cows. There were selected dairy cows with the longest period of lactation or already drying off, and especially dairy cows with various health problems for study. Limiting values of weight changes were established after assembling a general equation of mass curve. In the sphere of the diseases there was manifested only ketosis in the weight curve with a loss of 10.2 kg / day (38% weight loss. Additionally, the completion of growth during the first 2 periodes of lactations and weight gain due to advanced pregnancy were confirmed. The maximum daily weight difference recorded in healthy animals was 7 %, equivalent to 40 - 45 kg. The results of the study will be applied for compiling algorithm that will be implemented in the complete management system of cattle breeding, monitoring the dairy cows every day and highlight possible deviations exceeding of physiological changes in weight.
Coleman, D A; Thayne, W V; Dailey, R A
We conducted two studies to determine how herd management practices and traits of individual cows affect performance of the herd and of the cow within a herd. Management practices, reproductive performance of the herd, and relationships between management and reproductive performance were characterized on 83 dairy farms with 7596 cows. Data included 21 management variables (e.g., facilities, herd health program, estrous detection program) and 8 performance variables obtained from Dairy Herd Improvement or unofficial records (e.g., size of herd, production, days open). Although varying among herds, annual average herd incidences of reproductive disorders and reproductive performance were similar to those reported. Managerial practices influenced incidences of retained placenta and uterine infection, days open of cows not bred and of all cows, services per conception, and percentages of herd open more than 100 days and culled for low production. Veterinarian was the most consistent variable influencing herd reproductive performance. Data also were collected from production and lifetime records of 2532 cows in 19 herds. Reproductive performance was affected by season of calving, production, maturity, and reproductive disorders. Several cows with extremely poor reproductive records were maintained.
Poulton, P J; Vizard, A L; Anderson, G A; Pyman, M F
To investigate the relative importance in downer cows of the primary cause of recumbency in comparison with secondary complications. Downer dairy cows were monitored during their recumbency under field conditions in South Gippsland, Victoria, Australia. The cause of the original recumbency of the 218 cows was determined and secondary damage, status on day 7 and final outcome were recorded. Some type of secondary damage was found in 183/218 (84%) cows, of which 173/218 (79%) had damage deemed to be clinically important. By day 7, 52 (24%) had recovered and 69 (32%) eventually recovered. Of the 149 (68%) cows that were euthanased or died, 23 (15%) were deemed to have been lost solely from the primary cause, 107 (72%) from secondary damage and 19 (13%) from a combination of both. There was no difference in recovery among the five broad groups of causes of primary recumbency. Secondary damage was very common and presented in a large variety of ways, with many cows having multiple types of secondary damage concurrently. For most cows the secondary damage was more important than the initial primary damage in determining their fate. © 2016 Australian Veterinary Association.
Bishop-Williams, Katherine E.; Berke, Olaf; Pearl, David L.; Hand, Karen; Kelton, David F.
Background Heat stress is a physiological response to extreme environmental heat such as heat waves. Heat stress can result in mortality in dairy cows when extreme heat is both rapidly changing and has a long duration. As a result of climate change, heat waves, which are defined as 3 days of temperatures of 32 ?C or above, are an increasingly frequent extreme weather phenomenon in Southern Ontario. Heat waves are increasing the risk for on-farm dairy cow mortality in Southern Ontario. Heat st...
Sartori, Cristina; Mazza, Serena; Guzzo, Nadia; Mantovani, Roberto
In some species females compete for food, foraging territories, mating, and nesting sites. Competing females can exhibit morphological, physiological, and behavioral adaptations typical of males, which are commonly considered as secondary sexual traits. Competition and the development of traits increasing competitiveness require much energy and may exert adverse effects on fecundity and survival. From an evolutionary perspective, positive selection for increased competitiveness would then result in evolution of reduced values for traits related to fitness such as fecundity and survival. There is recent evidence for such evolutionary trade-offs involving male competition, but no study has considered competing females so far. Using data from competitions for dominance in cows (Bos taurus), we found negative genetic correlations between traits providing success in competition, that is, fighting ability and fitness traits related to milk production and with fertility (the inverse of parity-conception interval). Fighting ability also showed low but positive genetic correlations with "masculine" morphological traits, and negative correlations with "feminine" traits. A genetic change in traits over time has occurred due to selection on competitiveness, corresponding to an evolutionary process of "masculinization" counteracting the official selection for milk yield. Similar evolutionary trade-off between success in competition and fitness components may be present in various species experiencing female competition. © 2015 The Author(s). Evolution © 2015 The Society for the Study of Evolution.
Full Text Available The work presents the results of investigations of the effect of moderate heat stress on the acidobasal status in high-yield dairy cows in early lactation. Improving performance in high-yield dairy cows increases their inclination toward metabolic disorders. The most likely to be affected is the acid-base balance, in particular when cows are exposed to heat stress. Investigations so far have shown that the taking of urine samples and their analysis is the best and fastest way to diagnose disorders in the acid-base balance. Investigations were carried out on 7 clinically healthy cows of the Holstein- Friesian breed in the phase of early lactation, 30 to 40 days (on days 30, 33 and 40 following calving during the summer period, during the month of July, when there were significant variations in daily and nightly temperatures. The cows were in the second and fourth lactation, the annual milk yield was 8000 L milk per cow. The average daily milk production in the early phase of lactation ranged from 35 to 40 L. The cows were fed mixed rations (TMR twice daily. Lucerne hay in limited quantities was given to the cows prior to the mixed feed ration. The ration for this animal category was optimized on the grounds of the daily milk production. The balance of cations and anions in the feed ration stood at 95 mEq/kg DM. The results clearly demonstrate the cows' response to moderate heat stress through the defense parameters in urine (urine pH, NABE (net-acid-base-excretion, acids, bases, ammonium ion (NH4. The determination of the kidney NABE yields more correct data than the urine pH on acidotic conditions. The results of examinations of the urine pH do not show any digressions from physiological values and are approximately the same in all cows during the investigated periods. Normal NABE values are from 100-200 mmol/L. Burdening with acid products results in a NABE range from 0-100mmol/L, and metabolic acidosis results in NAB<0 mmol/L. NABE in the cows
Full Text Available The aim of the study was to examine the development of preovulatory follicles in pregnant and non-pregnant high yielding cows. The treatment by supergestran and oestrophan was used to synchronize the estrous cycle. Ovaries were monitored by transrectal ultrasonography. The linear increase of preovulatory follicles was observed in pregnant (P < 0,001 and non-pregnant (P < 0,001 cows during 8 days before ovulation. In conclusion, preovulatory follicles in pregnant and non-pregnant high yielding cows developed similarly.
Jones, William J; Johnson, Shannon B; Rouse, Greg W; Vrijenhoek, Robert C
Bone-eating worms of the genus Osedax colonized and grew on cow bones deployed at depths ranging from 385 to 2893 m in Monterey Bay, California. Colonization occurred as rapidly as two months following deployment of the cow bones, similar to the time it takes to colonize exposed whalebones. Some Osedax females found on the cow bones were producing eggs and some hosted dwarf males in their tubes. Morphological and molecular examinations of these worms confirmed the presence of six Osedax speci...
Full Text Available This work was carried out to evaluate the physiological thermoregulation responses of Girolando-breed dairy cows kept in feedlot during summertime. A total of 15 animals were used, belonging to three genetic groups: 1/2, 5/8 and 3/4 Holstein-Gir (HG, with five cows from each genetic group, distributed in a completely randomized design, in subplots. The physiological parameters were sweat rate (SR, respiratory rate (RR, epidermis temperature (ET and coat surface temperature (CST, and were evaluated once a week, during nine weeks, from December 2009 to February 2010, totaling 135 observations. Throughout the experimental period, climatic data were recorded using an automated weather station, and later used to calculate the different thermal comfort indexes: THI (temperature-humidity index, BGHI (black globe temperature and humidity index and RTL (radiant thermal load. 1/2 HG animals showed the highest SR and lowest RR, ET and CST of all three genetic groups, while 3/4 HG cows had the lowest mean SR and highest ET and CST. 5/8 HG cows had intermediate values for SR, ET and CST. Mean RR values did not differ between 5/8 and 3/4 HG cows. It was concluded that animals from genetic groups 3/4 and 5/8 HG showed greater sensibility to heat stress than 1/2 HG cows.
Jones, R W
Engineers and physiologists working together in experimental and theoretical studies predict that the application of system analysis to biological processes will increase understanding of these processes and broaden the base of system theory. Richard W. Jones, professor of electrical engineering at Northwestern University, Evanston, Illinois, and John S. Gray, professor of physiology at Northwestern's Medical School, discuss these developments. Their articles are adapted from addresses delivered in Chicago in November 1962 at the 15th Annual Conference on Engineering in Medicine and Biology.
Full Text Available Adipose tissue has a central role in the regulation of metabolism in dairy cows, and many proteins expressed in this tissue are involved in metabolic responses to stress (Peinado et al., 2012 . Environmental heat stress is one of the main stressors limiting production in dairy cattle (Fuquay, 1981; West, 2003 [2,3], and there is a complex interaction between heat stress and the transition period from late pregnancy to onset of lactation, which is manifested in heat-stressed late-gestation cows (Tao and Dahl, 2013 . We recently defined the proteome of adipose tissue in peripartum dairy cows, identifying 586 proteins of which 18.9% were differentially abundant in insulin-resistant compared to insulin-sensitive adipose tissue (Zachut, 2015 . That study showed that proteomic techniques constitute a valuable tool for identifying novel biomarkers in adipose tissue that are related to metabolic adaptation to stress in dairy cows. The objective of the present work was to examine the adipose tissue proteome under thermo-neutral or seasonal heat stress conditions in late pregnant dairy cows. We have collected subcutaneous adipose tissue biopsies from 10 late pregnant dairy cows during summer heat stress and from 8 late pregnant dairy cows during winter season, and identified and quantified 1495 proteins in the adipose tissues. This dataset of adipose tissue proteome from dairy cows adds novel information on the variety of proteins that are abundant in this tissue during late pregnancy under thermo-neutral as well as heat stress conditions. Differential abundance of 107 (7.1% proteins was found between summer and winter adipose. These results are discussed in our recent research article (Zachut et al., 2017 .
Chaidate, Inchaisri; Somchai, Chanpongsang; Jos, Noordhuizen; Henk, Hogeveen
Subacute ruminal acidosis in dairy cows occurs when ruminal pH is below about 5.5. However, the exact threshold level of ruminal pH affecting cow health is still in debate. This investigation was carried out in 505 cows within 31 farms. The postpartum disorders, including dystocia, retained placenta, anestrus, cystic ovary, metritis, clinical mastitis and lameness, were analyzed. Ruminal pH, serum beta-hydroxy butyrate (SBHB), serum urea nitrogen and body condition score (BCS) were measured once during the 3 to 6 weeks postpartum, while BCS was determined once more at 1 week before calving. Ruminal pH was determined by ruminocentesis technique. The ruminal pH was evaluated to study the association with BCS, SBHB and postpartum disorders using linear regression in a generalized linear mixed model with farm as a random effect. The results show that low ruminal pH was associated with dystocia, metritis and lameness. Moreover, a low ruminal pH can be found in cows with a high loss of BCS after calving and also in cows with low SBHB postpartum. These findings confirmed the feasibility of the ruminocentesis technique and the association of low ruminal pH on various postpartum disorders at the individual cow level. However, the consequences of low ruminal pH on dairy cow health still needs more exploration for a better understanding of the physiological mechanisms. © 2014 Japanese Society of Animal Science.
Gomez, N A; Conley, A J; Robinson, P H
This study aimed to contribute to understanding the interface between reproductive and nutritional energetic physiology in contemporary dairy cattle. Multiparous Holstein cows (n = 32) between 70 and 180 days in milk were used in a study starting 10 d prior to the artificial insemination (AI) date and were estrous synchronized using a hormonal regimen. Fourteen cows were determined pregnant on day 39 post-AI. Coccygeal blood samples of all cows were collected on d -10 and -3 prior to AI to determine estrous cyclicity, as well as at AI and at 6, 13 and 20 d post-AI. Milk progesterone was measured 20 d post-AI, and body condition was scored (BCS; 1-5 scale) on days -10, 0, 13 and 27 relative to AI. Blood non-esterified fatty acid concentrations, measured on the same days as BCS, and changes of BCS from d -10 to AI were not predictive of pregnancy outcome. The BCS of cows on the day of AI was greater (P = 0.02) for pregnant cows with an approximate minimum BCS for a high probability of conception being 2.50. Serum progesterone concentrations of pregnant cows were greater (P commercial high-producing multiparous dairy cattle may be partly due to breeding cows that have insufficient BCS to support pregnancy. Published by Elsevier B.V.
Gleidiana Amélia Pontes de Almeida
Full Text Available Objective The study aimed to evaluate sugarcane bagasse as roughage in lactating cow on feed intake, digestibility, ingestive behavior, milk production and composition, and microbial protein synthesis. Methods Ten Girolando cows at initial body weight of 450±25.6 kg and at 143.7±30.7 days in milk were assigned in two 5×5 Latin square designs. Five 21-day experimental periods were adopted (1° to 14-day: diets adaptation period; 15° to 21-day: data collection and sampling period. The diets consisted of four different levels of sugarcane bagasse (45%, 50%, 55%, and 60% and a control diet, commonly adopted in the region, based on spineless cactus (25% sugarcane bagasse, formulated to meet 12 kg/d milk yield. Results The dry matter (DM, organic matter (OM, and total digestible nutrients intakes and DM and OM digestibilities observed for 45% and 50% bagasse inclusion were similar to control diet, while that 55% and 60% bagasse inclusion were lower. Cows fed control diet, and bagasse diets of 45%, and 50% levels had the nutritional requirements attended, that guaranteed 12 kg/d of milk yield. The crude protein intake and digestibility of cows fed 45%, 50%, and 55% of bagasse inclusion were similar to control diet. The neutral detergent fiber (NDF intake and digestibility differ for all bagasse diets related to control diet, while the non-fiber carbohydrates intake and digestibility for cows fed 45% of bagasse were similar for control diet. The intakes and digestibilities of nutrients decreased linearly in function of bagasse inclusion; NDF and indigestible NDF intakes did not vary. The ruminating time, feeding and rumination efficiency, microbial protein synthesis and milk yield decreased linearly with sugarcane bagasse inclusion. Conclusion Sugarcane bagasse decreases milk production; however, its inclusion level in between 45% to 50% associated to concentrate could replace diets based on spineless cactus for crossbred dairy cow's producing 12 kg
Trevisi, E; Amadori, M; Cogrossi, S; Razzuoli, E; Bertoni, G
Increased disease rates are commonly reported among high-yielding dairy cows in the transition period, extending from 3 weeks before to 3 weeks after calving, and characterized by the occurrence of an inflammatory response in terms of both positive and negative acute phase proteins (APP+ and APP-). To determine the above inflammatory response, the authors had developed the Liver Functionality Index (LFI), which defines the above condition on the basis of some APP- responses (albumin, cholesterol sensu stricto+bilirubin) during the first month of lactation. In this respect, low LFI values are associated to a high inflammatory response and vice versa. The relationship between LFI and inflammatory cytokine response was investigated from day -28 to day +28 with respect to calving in 12 periparturient dairy cows showing the six highest and six lowest LFI values within a cohort of 54 high-yielding dairy cows. The hypothesis being tested was that LFI and APP- on the whole could be used as readout of successful vs. non-successful adaptation to the transition period, with a strong association to disease occurrence. In fact, low LFI cows experienced many more disease cases (13 vs. 3 in high LFI Group) and related drug treatments till day +28. Interleukin-6 (IL-6) serum concentrations were always higher in low LFI cows (Pcows at risk in the transition period toward an improved farm management. Also, our study indicates that disease cases in periparturient, high-yielding dairy cows are correlated with signs of accentuated IL-6 response and other markers of inflammatory phenomena. These likely start in the late lactation period or around dry-off, as suggested by our prepartal data, and proceed at much greater levels after calving. Copyright © 2011 Elsevier Ltd. All rights reserved.
de Almeida, Gleidiana Amélia Pontes; de Andrade Ferreira, Marcelo; de Lima Silva, Janaina; Chagas, Juana Catarina Cariri; Véras, Antônia Sherlânea Chaves; de Barros, Leonardo José Assis; de Almeida, Gledson Luiz Pontes
The study aimed to evaluate sugarcane bagasse as roughage in lactating cow on feed intake, digestibility, ingestive behavior, milk production and composition, and microbial protein synthesis. Ten Girolando cows at initial body weight of 450±25.6 kg and at 143.7±30.7 days in milk were assigned in two 5×5 Latin square designs. Five 21-day experimental periods were adopted (1° to 14-day: diets adaptation period; 15° to 21-day: data collection and sampling period). The diets consisted of four different levels of sugarcane bagasse (45%, 50%, 55%, and 60%) and a control diet, commonly adopted in the region, based on spineless cactus (25% sugarcane bagasse), formulated to meet 12 kg/d milk yield. The dry matter (DM), organic matter (OM), and total digestible nutrients intakes and DM and OM digestibilities observed for 45% and 50% bagasse inclusion were similar to control diet, while that 55% and 60% bagasse inclusion were lower. Cows fed control diet, and bagasse diets of 45%, and 50% levels had the nutritional requirements attended, that guaranteed 12 kg/d of milk yield. The crude protein intake and digestibility of cows fed 45%, 50%, and 55% of bagasse inclusion were similar to control diet. The neutral detergent fiber (NDF) intake and digestibility differ for all bagasse diets related to control diet, while the non-fiber carbohydrates intake and digestibility for cows fed 45% of bagasse were similar for control diet. The intakes and digestibilities of nutrients decreased linearly in function of bagasse inclusion; NDF and indigestible NDF intakes did not vary. The ruminating time, feeding and rumination efficiency, microbial protein synthesis and milk yield decreased linearly with sugarcane bagasse inclusion. Sugarcane bagasse decreases milk production; however, its inclusion level in between 45% to 50% associated to concentrate could replace diets based on spineless cactus for crossbred dairy cow's producing 12 kg/d of milk.
Full Text Available The objective of the work was to examine the intensity of the local immune response of the mammary gland and the changes in the differential blood count of chronically infected cows. An experiment was performed on a group of cows with Q fever serologically proven using the ELISA test (IDEXX. Based on the ELISA test results, an experimental group of ten infected cows was formed. Blood was sampled from the experimental cows, and cumulative milk samples were taken. The number of erythrocytes was determined spectrophotometrically, and the number of leucocytes using the method according to Bürker - Türk. The blood analysis established an increased number of erythrocytes, while the number of leucocytes was within the limits of physiological values. The milk samples were used for the determination of the number of somatic cells using flow cytometric measurements. The processing of the milk samples established an average number of somatic cells of 853.000 /mL milk.
Dänicke, Sven; Meyer, Ulrich; Kersten, Susanne; Frahm, Jana
The immune system is particularly challenged in transition cows as marked physiological changes occur in this period which are driven by late gestation, partus and onset of lactation. As a consequence, the metabolic and nutritional state of the cow also changes significantly with possible implications for the plasticity and flexibility of the immune system. In order to understand how the balance between metabolism, nutritional status and the immune system is maintained under challenging conditions, such as an infection, various animal models can be used which specifically manipulate the nutritional status through various feeding and management strategies. Such models aim at exploring the immunological response to a challenge under largely varying nutritional and metabolic states. As energy balance (EB) is strongly associated both with the metabolic state and with the immunoreactivity of the cows the manipulation of the EB by either influencing energy intake or energy excretion with milk, or by both, offers model opportunities for studying EB effects on the immune system. For example, assigning cows with a higher body condition score (BCS) at least 6 weeks prior to calving to an energy-dense diet exceeding the energy requirement in combination with a decelerated increase in the concentrate feed proportion post partum was shown to be effective in inducing a ketotic metabolic state under ad libitum feeding conditions. Compared to an adequately managed control group this model allows studying immune responses in the transit period and in dependence on dietary interventions. Copyright © 2018 Elsevier Ltd. All rights reserved.
Sawsan Ahmed Elhouri Ahmed; Mubarak Dirar Abdallah2
In this work five samples of (cow and sheep's bones) were prepared to powders in a period of crashing (10 up to 50 sec); weight = 56.73mg To find values of: Refractive index Energy gap And Electrical Conductivity
Jónsson, Ragnar Ingi
This report covers an initial study on the modelling of cow behaviour using stochastic automata with the aim of detecting lameness. Lameness in cows is a serious problem that needs to be dealt with because it results in less profitable production units and in reduced quality of life...... for the affected livestock. By featuring training data consisting of measurements of cow activity, three different models are obtained, namely an autonomous stochastic automaton, a stochastic automaton with coinciding state and output and an autonomous stochastic automaton with coinciding state and output, all...... of which describe the cows' activity in the two regarded behavioural scenarios, non-lame and lame. Using the experimental measurement data the different behavioural relations for the two regarded behavioural scenarios are assessed. The three models comprise activity within last hour, activity within last...
... Disease (CWD) Prion Diseases Relationship with BSE (Mad Cow Disease) Evidence Recommend on Facebook Tweet Share Compartir ... macaque monkeys inoculated with brain tissue obtained from cattle with BSE had clinical and neuropathological features strikingly ...
Jones, William J; Johnson, Shannon B; Rouse, Greg W; Vrijenhoek, Robert C
Bone-eating worms of the genus Osedax colonized and grew on cow bones deployed at depths ranging from 385 to 2893m in Monterey Bay, California. Colonization occurred as rapidly as two months following deployment of the cow bones, similar to the time it takes to colonize exposed whalebones. Some Osedax females found on the cow bones were producing eggs and some hosted dwarf males in their tubes. Morphological and molecular examinations of these worms confirmed the presence of six Osedax species, out of the eight species presently known from Monterey Bay. The ability of Osedax species to colonize, grow and reproduce on cow bones challenges previous notions that these worms are 'whale-fall specialists.'
The prevalence of allergic disease has increased markedly over the last 50 years. Food allergy usually manifests in early childhood as part of the so-called atopic march and most commonly includes one or more of the following foods: cow's milk, hen's egg, soy, peanuts and tree nuts, wheat, sesame seed, kiwi fruit and seafood. In the UK about 2% of infants develop cow's milk protein allergy (CMPA), but as many as 15% of infants present with symptoms suggestive of an adverse reaction to cow's milk protein. The diagnosis of CMPA is based on one or more of the following: a detailed clinical history, allergy test results (skin prick testing [SPT] and/or specific immunoglobulin E [IgE]) and, if required, supervised incremental milk challenges. The majority of UK primary care centres do not have access to these tests and may also be unfamiliar with the interpretation or results. In addition, they do not have the facilities for supervised food challenges. Empirical treatment is often required pending confirmation of allergy or referral to a specialist centre, but requires clear guidelines. No consensus guidelines currently exist for the diagnosis and management of CMPA in the UK. An international task force has recently published proposed guidelines for the management of CMPA. These provide separate algorithms covering the diagnosis and management of CMPA for both breast-fed and formula-fed infants and discuss the use of hypoallergenic formulae, elimination diets and diagnostic tests. Revisions and adaptations for the UK market are required and are discussed in this article.
Acharya, C N
A description of experimental work in India and a variety of digesters is given. A small plant at the Indian Agricultural Research Institute, New Delhi, was built in 1941 and has been operating on 4.5 kg of manure/day for 12 years. A plant capable of handling 1800--2300 kg of cow dung per day was built by Prof. V. N. Joshi on the sugarcane estate of Walchandnagar Industries. Shri Chandra Das Gupta experimented with bamboo thatch cylinders sunk into the ground to form tanks, and with bamboo thatch plastered with earth and cement to form gasholders. The West Bengal Government Farm at Harenghatta houses a digestion plant consisting of a series of crude-oil drums through which the slurry passes, gas being collected from all drums.
On display at Fort l'Écluse from 19 June to 18 September, the works of Augenblick combine photography, sound and video to create a striking instant parallel between the "real world" and the world of particle physics. The cows are grazing quietly, blissfully unaware of the mini-Big Bang occurring silently 100 metres beneath them. Curious to compare these two worlds - the visible and the invisible, day-to-day life and particle physics - Laurent Mulot, a multi-disciplinary artist whose work delves into some of the planet's more unusual places, has come up with a unique artistic concept. Called Augenblick (German for "instant"), to emphasise that the images relate to the same point in time, his project uses photography, video and sound to superimpose scenes from everyday life and scenes from science. "The idea came to me in June 2008," says Laurent Mulot, "when Jean-Paul Martin, a research scientist at the Institute of Nuclear...
Hardie, L C; VandeHaar, M J; Tempelman, R J; Weigel, K A; Armentano, L E; Wiggans, G R; Veerkamp, R F; de Haas, Y; Coffey, M P; Connor, E E; Hanigan, M D; Staples, C; Wang, Z; Dekkers, J C M; Spurlock, D M
The objective of this study was to identify genomic regions and candidate genes associated with feed efficiency in lactating Holstein cows. In total, 4,916 cows with actual or imputed genotypes for 60,671 single nucleotide polymorphisms having individual feed intake, milk yield, milk composition, and body weight records were used in this study. Cows were from research herds located in the United States, Canada, the Netherlands, and the United Kingdom. Feed efficiency, defined as residual feed intake (RFI), was calculated within location as the residual of the regression of dry matter intake (DMI) on milk energy (MilkE), metabolic body weight (MBW), change in body weight, and systematic effects. For RFI, DMI, MilkE, and MBW, bivariate analyses were performed considering each trait as a separate trait within parity group to estimate variance components and genetic correlations between them. Animal relationships were established using a genomic relationship matrix. Genome-wide association studies were performed separately by parity group for RFI, DMI, MilkE, and MBW using the Bayes B method with a prior assumption that 1% of single nucleotide polymorphisms have a nonzero effect. One-megabase windows with greatest percentage of the total genetic variation explained by the markers (TGVM) were identified, and adjacent windows with large proportion of the TGVM were combined and reanalyzed. Heritability estimates for RFI were 0.14 (±0.03; ±SE) in primiparous cows and 0.13 (±0.03) in multiparous cows. Genetic correlations between primiparous and multiparous cows were 0.76 for RFI, 0.78 for DMI, 0.92 for MBW, and 0.61 for MilkE. No single 1-Mb window explained a significant proportion of the TGVM for RFI; however, after combining windows, significance was met on Bos taurus autosome 27 in primiparous cows, and nearly reached on Bos taurus autosome 4 in multiparous cows. Among other genes, these regions contain β-3 adrenergic receptor and the physiological candidate gene
Smith, W B; Coffey, K P; Rhein, R T; Kegley, E B; Philipp, D; Powell, J G; Caldwell, J D; Young, A N
Coproduct feedstuffs offer a unique and potentially profitable avenue for cattle feeding strategies. However, research is lacking in the evaluation of varying coproducts on ruminal fermentation and digestive characteristics when included as the major component of the diet of cows. Our objective was to determine the effect of coproduct feedstuffs as a forage replacement on digestive and fermentative characteristics of cows. Eight ruminally fistulated cows (672 ± 32.0 kg initial BW and approximately 9 yr of age) were stratified by BW and randomly allocated to 1 of 4 diets (2 cows∙diet∙period) in a 2-period study: soybean hulls (SH), distillers' dried grains with solubles (DG), an isoenergetic mixture of soybean hulls and distillers' dried grains with solubles (MX), or ad libitum hay plus 0.9 kg/d of an isoenergetic mixture of soybean hulls and distillers' dried grains with solubles (HY). Diets were formulated to meet the ME requirements of a similar, companion study. Coproduct amounts were increased over a 14-d period. This was followed by a 14-d adaptation to diet and facilities and 5 d of total fecal collections. On the final day of fecal collections, rumen fluid was sampled immediately prior to feeding and 2, 4, 6, 8, 10 and 12 h after feeding for measurement of rumen VFA and ammonia concentrations. Intake of DM and OM was not different ( ≥ 0.28) among treatments, but digestibilities of DM, OM, NDF, and ADF were improved ( cows offered HY than for cows offered the coproduct diets, greater for cows offered SH than for cows offered DG, and for the mean of SH and DG vs. MX. Ruminal retention time was greater ( cows offered the coproduct diets than for cows offered HY and greater for cows offered DG than for cows offered SH. Total VFA averaged across sampling times were greatest ( cows offered SH, and ruminal ammonia N was greatest ( cows offered either DG or MX at all sampling times. Based on these data, coproduct feedstuffs may be fed to meet the energy
Turvey, S.T; Risley, C.L
Steller's sea cow, a giant sirenian discovered in 1741 and extinct by 1768, is one of the few megafaunal mammal species to have died out during the historical period. The species is traditionally considered to have been exterminated by ‘blitzkrieg’-style direct overharvesting for food, but it has also been proposed that its extinction resulted from a sea urchin population explosion triggered by extirpation of local sea otter populations that eliminated the shallow-water kelps on which sea cow...
Guatteo, Raphaël; Beaudeau, François; Joly, Alain; Seegers, Henri
While shedding routes of Coxiella burnetii are identified, the characteristics of Coxiella shedding are still widely unknown, especially in dairy cattle. However, this information is crucial to assess the natural course of Coxiella burnetii infection within a herd and then to elaborate strategies to limit the risks of transmission between animals and to humans. The present study aimed at (i) describing the characteristics of Coxiella burnetii shedding by dairy cows (in milk, vaginal mucus, faeces) in five infected dairy herds, and at (ii) investigating the possible relationships between shedding patterns and serological responses. A total of 145 cows were included in a follow-up consisting of seven concomitant samplings of milk, vaginal mucus, faeces and blood (Day 0, D7, D14, D21, D28, D63, D90). Detection and quantification of Coxiella burnetii titres were performed in milk, vaginal mucus and faeces samples using real-time PCR assay, while antibodies against Coxiella were detected using an ELISA technique. For a given shedding route, and a given periodicity (weekly or monthly), cows were gathered into different shedding kinetic patterns according to the sequence of PCR responses. Distribution of estimated titres in Coxiella burnetii was described according to shedding kinetic patterns. Coxiella burnetii shedding was found scarcely and sporadically in faeces. Vaginal mucus shedding concerned almost 50% of the cows studied and was found intermittently or sporadically, depending on the periodicity considered. Almost 40% of cows were detected as milk shedders, with two predominant shedding patterns: persistent and sporadic, regardless of the sampling periodicity. Significantly higher estimated titres in Coxiella burnetii were observed in cows with persistent shedding patterns suggesting the existence of heavy shedder cows. These latter cows were mostly, persistently highly-seropositive, suggesting that repeated serological testings could be a reliable tool to screen
High producing dairy cows struggle to meet energy demands and handle various transitional changes in late gestation and early lactation. Negative energy balance in early lactation is inevitable and metabolic disorders may follow as a consequence of a deep negative energy balance. This thesis studies associations between blood profiles and body condition score (BCS) in dairy cows, and displaced abomasum (DA) or clinical ketosis, and investigates risk factors for the two diseases at the herd le...
Full Text Available World milk production has a great economical effect being in the function of human food production and nutrition. Milk is obtained from cows, buffalos, sheeps, goats, camels and donkey with cow's milk production dominating. The world milk production in 2000 was 568.480 thousands of tons of all types of milk of which 484.895 thousands tons are cow's milk with a total of 85.30 % of the world milk production. Buffalo's milk production is on the second place with 61.913 thousands of tonnes (10.89 % production capacity. On the three continents (Europe, North America and Asia 81.82 % of total cow's milk production is located. Developed countries produce 50 % of total milk production, while higher milk production is forecast for the developing countries. The EU countries participate with 23.72 % in the world milk production and with 55.60 % on the European area. High annual lactation production, under selected cow's milk production, of above 6000 kg is located in developed countries, where annual participation of Israel accounts for over 10000 kg of milk per cow. Commercial milk production of genetics cattle accounts from 80 % to 85 %. Milk quality, with regard to milk fat and proteincontent, in developed countries is above an average value. With the annual milk production of 7000 kg of cow's milk, up to 294 kg of milk fat and 238 kg of protein are produced. Due to milk characteristics as agro-food product, milk and dairy products manufacture and transportation are in details regulated with existing quality standards. 95 % of the EU milk producers fulfil international hygienic rules on milk safety standards (somatic cells, microorganisms. With regard to long term development, until 2030, changes on herd management (outdoor and indoor exposure, between continents, will occur. In 2030, the world milk production is forecast to increase by 64%, with cow's milk production of 765.9 million tonnes.
Nielsen, B.H.; Jacobsen, S.; Andersen, P.H.; Niewold, T.A.; Heegaard, P.M.H.
The concentrations of the two acute phase proteins, serum amyloid A and haptoglobin, in serum and milk were compared in 10 cows with clinical mastitis, 11 cows with extramammary inflammatory conditions and 10 clinically healthy control cows. The concentrations of both acute phase proteins were
Thomsen, Peter; Sørensen, Jan Tind
a random sample of 196 Danish dairy farmers that had reported a dead cow to the Danish Cattle Database in 2002 and 196 dairy farmers that had reported a dead cow in 2006. Our objectives were to evaluate the proportion of euthanized cows, changes in the behaviour of farmers regarding euthanasia of cows over...... the years and possible reasons for these changes. Results It seems that the threshold for euthanasia of cows among farmers has changed. Farmers generally reported a lower threshold for euthanasia compared to 5-10 years ago. Conclusions The threshold for euthanasia of cows has, according to the dairy farmers...
Calamari, L.; Petrera, F.; Stefanini, L.; Abeni, F.
The aim of this paper was to evaluate the effects of three different feeding management (FM) schedules on physiological markers of heat stress (HS), metabolic conditions, milk yield and quality during the hot season in dairy cows. The study involved 27 mid-lactating cows, subdivided in three homogeneous groups differing in feeding time and frequency: total mixed ration (TMR) delivered once daily in the morning (M); twice daily, half in the morning and half in the evening (ME); once daily in the evening (E). During the trial, blood samples were collected in the morning (a.m.) and in the evening (p.m.), breathing rate (BR), rectal temperature (RT), and milk yield were recorded and individual milk samples were collected. Microclimate data indicated that cows were subjected to mild-moderate HS. During the hotter days, cows receiving M treatment showed higher values of RT (38.97 °C vs 38.68 °C and 38.62 °C, in ME and E) and BR (71.44 vs 66.52 and 65.26 breaths min-1, in ME and E), a.m. plasma glucose was lower in M (3.69 vs 3.83 and 3.83 mmol L-1, in ME and E) and a.m. plasma urea was lower in E (4.82 vs 5.48 and 5.35 mmol L-1, in M and ME). Milk yield was unaffected by FM, as well as milk composition and cheese-making properties. Only milk protein content and yield were higher in M (3.42 vs 3.36 and 3.27 g 100 mL-1; and 1.11 vs 1.08 and 1.02 kg day-1, for ME and E). Our results on cow physiology indicate that M seems a less suitable FM to match cow welfare during the summer season.
Inchaisri, C.; Jorritsma, R.; Vernooij, J.C.M.; Vos, P.L.A.M.; Weijden, van der G.C.; Hogeveen, H.
The objective of this research was to determine the contribution of cow factors to the probability of successful insemination accounting for the serial number of inseminations in analysis. The investigation was performed with 101 297 insemination records in 51 525 lactations of different cows from
de Andrade Ferrazza, Rodrigo; Mogollón Garcia, Henry David; Vallejo Aristizábal, Viviana Helena; de Souza Nogueira, Camilla; Veríssimo, Cecília José; Sartori, José Roberto; Sartori, Roberto; Pinheiro Ferreira, João Carlos
Heat stress (HS) adversely influences productivity and welfare of dairy cattle. We hypothesized that the thermoregulatory mechanisms vary depending on the exposure time to HS, with a cumulative effect on the adaptive responses and thermal strain of the cow. To identify the effect of HS on adaptive thermoregulatory mechanisms and predictors of caloric balance, Holstein cows were housed in climate chambers and randomly distributed into thermoneutral (TN; n=12) or HS (n=12) treatments for 16 days. Vaginal temperature (VT), rectal temperature (Tre), respiratory rate (RR), heart rate (HR), and dry matter intake (DMI) were measured. The temperature and humidity under TN were 25.9±0.2°C and 73.0±0.8%, respectively, and under HS were 36.3±0.3°C and 60.9±0.9%, respectively. The RR of the HS cows increased immediately after exposure to heat and was higher (76.02±1.70bpm, pcows from the third day (8.27±0.33kgd -1 in the HS vs. 14.03±0.29kgd -1 in the TN, pheat exchange. The difference in the responses to acute and chronic exposure to HS suggests an adaptive response. Thus, intense thermal stress strongly influence thermoregulatory mechanisms and the acclimation process depend critically on heat exposure time. Copyright © 2017 Elsevier Ltd. All rights reserved.
Allen, J D; Hall, L W; Collier, R J; Smith, J F
Cattle show several responses to heat load, including spending more time standing. Little is known about what benefit this may provide for the animals. Data from 3 separate cooling management trials were analyzed to investigate the relationship between behavioral patterns in lactating dairy cows experiencing mild to moderate heat stress and their body temperature. Cows (n=157) were each fitted with a leg data logger that measured position and an intravaginal data logger that measures core body temperature (CBT). Ambient conditions were also collected. All data were standardized to 5-min intervals, and information was divided into several categories: when standing and lying bouts were initiated and the continuance of each bout (7,963 lying and 6,276 standing bouts). In one location, cows were continuously subjected to heat-stress levels according to temperature-humidity index (THI) range (THI≥72). The THI range for the other 2 locations was below and above a heat-stress threshold of 72 THI. Overall and regardless of period of day, cows stood up at greater CBT compared with continuing to stand or switching to a lying position. In contrast, cows lay down at lower CBT compared with continuing to lie or switching to a standing position, and lying bouts lasted longer when cows had lower CBT. Standing bouts also lasted longer when cattle had greater CBT, and they were less likely to lie down (less than 50% of lying bouts initiated) when their body temperature was over 38.8°C. Also, cow standing behavior was affected once THI reached 68. Increasing CBT decreased lying duration and increased standing duration. A CBT of 38.93°C marked a 50% likelihood a cow would be standing. This is the first physiological evidence that standing may help cool cows and provides insight into a communally observed behavioral response to heat. Copyright © 2015 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Devries, T J; Aarnoudse, M G; Barkema, H W; Leslie, K E; von Keyserlingk, M A G
Poor dairy cow hygiene has been consistently associated with elevated somatic cell count (SCC) and the risk of subclinical mastitis. The objective of this study was to determine the associations between dairy cow standing and lying behavior, barn hygiene, cow hygiene, and the risk of experiencing elevated SCC. Lactating Holstein dairy cows (n=69; 86 ± 51 DIM; parity: 2.0 ± 1.2; means ± SD), kept in 1 of 2 groups, were monitored over a 4-mo period. Each group contained 61 ± 1 (mean ± SD) cows over the study period; complete data were obtained from 37 and 32 animals within each respective group. Cows were housed in a sand-bedded, freestall barn with 2 symmetrical pens, each with a free cow traffic automatic milking system. To vary barn hygiene, in 4 consecutive 28-d periods, alley manure scrapers in each of the 2 pens were randomly assigned to frequencies of operation of 3, 6, 12, and 24 times per day. During the last 7 d of each period, cow hygiene (upper leg/flank, lower legs, and udder; scale of 1 = very clean to 4 = very dirty) and stall hygiene (number of 0.15×0.15-m squares contaminated with manure in a 1.20×1.65-m grid) were recorded. Standing and lying behavior of the cows were collected during those days using data loggers. Individual-cow SCC was recorded at the beginning and end of each 28-d period. Elevated SCC was used as an indicator of subclinical mastitis; incidence of elevated SCC was defined as having a SCC >200,000 cells/mL at the end of each 28-d period, when SCC was <100,000 cells/mL at the beginning of the period. Less frequent scraping of the barn alleys was associated with cows having poorer hygiene. Poor udder hygiene was associated with poor stall hygiene. Longer lying duration was associated with poor hygiene of the upper legs/flank and udder. Greater premilking standing duration was associated with poor udder hygiene and decreased frequency of lying bouts was associated with poor hygiene of the lower legs. Higher milk yield was
Mann, S; Leal Yepes, F A; Duplessis, M; Wakshlag, J J; Overton, T R; Cummings, B P; Nydam, D V
Overfeeding energy in the dry period can affect glucose metabolism and the energy balance of transition dairy cows with potential detrimental effects on the ability to successfully adapt to early lactation. The objectives of this study were to investigate the effect of different dry cow feeding strategies on glucose tolerance and on resting concentrations of blood glucose, glucagon, insulin, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) in the peripartum period. Cows entering second or greater lactation were enrolled at dry-off (57 d before expected parturition) into 1 of 3 treatment groups following a randomized block design: cows that received a total mixed ration (TMR) formulated to meet but not exceed energy requirements during the dry period (n=28, controlled energy); cows that received a TMR supplying approximately 150% of energy requirements during the dry period (n=28, high energy); and cows that were fed the same diet as the controlled energy group for the first 28 d, after which the TMR was formulated to supply approximately 125% of energy requirements until calving (n=28, intermediate energy). Intravenous glucose tolerance tests (IVGTT) with rapid administration of 0.25 g of glucose/kg of body weight were performed 28 and 10d before expected parturition, as well as at 4 and 21 d after calving. Area under the curve for insulin and glucose, maximal concentration and time to half-maximal concentration of insulin and glucose, and clearance rates were calculated. Insulin resistance (IR) indices were calculated from baseline samples obtained during IVGTT and Spearman rank correlations determined between IVGTT parameters and IR indices. Treatment did not affect IVGTT parameters at any of the 4 time points. Correlation between IR indices and IVGTT parameters was generally poor. Overfeeding cows energy in excess of predicted requirements by approximately 50% during the entire dry period resulted in decreased postpartum basal plasma glucose and
Watters, M E Alexandrea; Meijer, Karin M A; Barkema, Herman W; Leslie, Kenneth E; von Keyserlingk, Marina A G; Devries, Trevor J
Recent evidence suggests that the risk of intramammary infection in dairy cows is related to lying patterns. The objectives of this study were to quantify the standing and lying behavior of dairy cows milked 3×/d, determine the cow- and herd-level factors associated with these behaviors, and relate these findings to the risk of an elevated somatic cell count (SCC). Five commercial free-stall dairy herds in Eastern Ontario, milking 3×/d, were enrolled in a longitudinal study. Forty Holstein-Friesian cows/herd were randomly selected as focal animals based on days in milk (cow SCC was recorded at the beginning of each period and end of the final period. Elevated SCC (eSCC) was used as an indicator of subclinical mastitis. A new incident eSCC was defined as an individual cow that started the period with a SCC cows for hygiene and lameness. Throughout the course of the study, cows averaged 11.2h/d of lying time, split into 8.6 lying bouts/d that were on average 84.6 min in length. Later lactation cows had longer daily lying times that were split into fewer lying bouts of longer duration than cows earlier in lactation. Lame cows had longer daily lying times and lying bout durations than non-lame cows. Cows with greater milk yield had lower lying times than lower producing cows. Average post-milking standing time across the study herds was 103 min. Manipulation of feed (feed delivery or push-up) by the stockperson, in the hour before milking or shortly thereafter, resulted in the longest post-milking standing times. Over the study period, 48 new eSCC were detected, resulting in a mean herd incidence rate of 0.91 eSCC/cow-year at risk for all study herds. A non-linear relationship between post-milking standing time and eSCC incidence was found; compared to those cows that lie down cows that lie down for the first time >90 min after milking had a lower risk of acquiring a new eSCC. The risk of experiencing an eSCC was also increased in multiparous cows, and in those cows
Boro, Prasanta; Kumaresan, A; Pathak, Rupal; Patbandha, T K; Kumari, Susavi; Yadav, Asha; Manimaran, A; Baithalu, R K; Attupuram, Nitin M; Mohanty, T K
Retention of fetal membranes (RFM) adversely affects the production and reproduction potential of the affected cows leading to huge economic loss. Physiological separation of fetal membranes is reported to be an inflammatory process. The present study compared the concentrations of certain pro inflammatory cytokines [Interleukin 1β (IL-1), Interleukin 6 (IL-6), Interleukin 8 (IL-8) and Tumor necrosis factor α (TNF-α) between the cows that developed RFM (n=10) and the cows that expelled fetal membranes normally (n=10) to find out if they could serve as a predictive tool for RFM. Blood samples were collected from the cows from 30 days before expected parturition through day -21, day -14, day -7, day -5, day -3, day -1, on the day of parturition (day 0), day 1 postpartum and the pro-inflammatory cytokines were estimated in blood plasma by ELISA method. The IL-1β concentration was significantly lower (Pmembranes normally from 3 days before calving till the day of calving. The plasma concentrations of IL-6 and IL-8 were also lower (Pmembranes normally. It may be inferred that the concentrations of IL-1, IL-6, IL-8 and TNF-α around parturition were altered in cows developing RFM compared to those expelled fetal membranes normally. Copyright © 2015. Published by Elsevier B.V.
Khongdee, S.; Chaiyabutr, N.; Hinch, G.; Markvichitr, K.; Vajrabukka, C.
Fourteen animals of second and third lactation of Thai Friesian crossbred cows (87.5% Friesian × 12.5% Bos indicus) located at Sakol Nakhon Research and Breeding Centre, Department of Livestock Development, Ministry of Agriculture and Cooperatives, were divided randomly into two groups of seven each to evaluate the effects of evaporative cooling on reproductive and physiological traits under hot, humid conditions. Results indicated that installation of evaporating cooling in the open shed gave a further improvement in ameliorating heat stress in dairy cows in hot-wet environments by utilising the low humidity conditions that naturally occur during the day. The cows housed in an evaporatively cooled environment had both a rectal temperature and respiration rate (39.09°C, 61.39 breaths/min, respectively) significantly lower than that of the non-cooled cows (41.21°C; 86.87 breaths/min). The former group also had higher milk yield and more efficient reproductive performance (pregnancy rate and reduced days open) than the latter group. It is suggested that the non-evaporatively cooled cows did not gain benefit from the naturally lower heat stress during night time.
Cheng, Jianbo; Min, Li; Zheng, Nan; Fan, Caiyun; Zhao, Shengguo; Zhang, Yangdong; Wang, Jiaqi
This study was designed to investigate the effects of sudden cooling on the physiological responses of 12 heat-stressed Holstein dairy cows using an isobaric tags for relative and absolute quantification (iTRAQ) labeling approach. Plasma samples were collected from these cows during heat stress (HS), and after strong, sudden cooling in the summer (16 days later). We compared plasma proteomic data before and after sudden cooling to identify the differentially abundant proteins. The results showed that sudden cooling in summer effectively alleviated the negative consequences of HS on body temperature and production variables. Expressions of plasma hemoglobin alpha and hemoglobin beta were upregulated, whereas lipopolysaccharide-binding protein (LBP) and haptoglobin were downregulated in this process. The increase of hemoglobin after cooling may improve oxygen transport and alleviate the rise in respiration rates in heat-stressed dairy cows. The decrease of LBP and haptoglobin suggests that the inflammatory responses caused by HS are relieved after cooling. Our findings provide new insight into the physiological changes that occur when heat-stressed dairy cows experience strong, sudden cooling.
Full Text Available Most experimental studies on animal stress physiology have focused on acute stress, while chronic stress, which is also encountered in intensive dairy cattle farming--e.g. in case of lameness--, has received little attention. We investigated heart rate (HR and heart rate variability (HRV as indicators of the autonomic nervous system activity and fecal glucocorticoid concentrations as the indicator of the hypothalamic-pituitary-adrenal axis activity in lame (with locomotion scores 4 and 5; n = 51 and non-lame (with locomotion scores 1 and 2; n = 52 Holstein-Friesian cows. Data recorded during the periods of undisturbed lying--representing baseline cardiac activity--were involved in the analysis. Besides linear analysis methods of the cardiac inter-beat interval (time-domain geometric, frequency domain and Poincaré analyses non-linear HRV parameters were also evaluated. With the exception of standard deviation 1 (SD1, all HRV indices were affected by lameness. Heart rate was lower in lame cows than in non-lame ones. Vagal tone parameters were higher in lame cows than in non-lame animals, while indices of the sympathovagal balance reflected on a decreased sympathetic activity in lame cows. All geometric and non-linear HRV measures were lower in lame cows compared to non-lame ones suggesting that chronic stress influenced linear and non-linear characteristics of cardiac function. Lameness had no effect on fecal glucocorticoid concentrations. Our results demonstrate that HRV analysis is a reliable method in the assessment of chronic stress, however, it requires further studies to fully understand the elevated parasympathetic and decreased sympathetic tone in lame animals.
Full Text Available Nazari A1, Noroozi H2, Movahedi M3, Khaksarian M1 1. Instructor, Department of Physiology, Faculty of Medicine, Lorestan University of Medical Sciences 2. Assistant Professor, Department of Mycology, Faculty of Medicine, Iran University of Medical Sciences 3. Assistant Professor, Department of Genetic Epidemiology, Faculty of Medicine, Lorestan University of Medical Sciences Abstract Background: Aflatoxin M1 is a hydroxylated form of aflatoxin B1 which is produced by Aspergillus flavus. This toxin is produced when cows or other ruminants eat foods contaminated with these mycotoxins and then excrete them in the milk. The toxin is a potent liver and kidney carcinogenetic agent. Materials and methods: Forty two raw cows milk samples from local sources of milk collection and forty samples of commercial pasteurized market milk from Khorramabad, Lorestan, Iran were collected in summer and winter season of 2005. Twenty-one cow milk samples and 20 pasteurized milk samples in each season were analyzed for the presence of aflatoxin M1 (AFM1 by HPLC immunoaffinity columns. Results: Four of 21 raw milk samples in summer showed AFM1 levels between 0.017-0.046 ng/ml and all samples (100% in winter showed the presence of AFM1 levels between 0.003-0.041ng/ ml. AFM1 was detected in 55% of market pasteurized cow milk samples ranging from 0.017 to 0.533 ng/ml in summer and 100% ranging from 0.005-0.0054 ng/ml in winter.,Only one of all milk samples of pasteurized milk in summer had toxin level (0.533 ng/ml more than the maximum permissive limit (0.5 ng/ml. No significant difference was observed among mean contamination level of raw and pasteurized cow milk in two seasons. Key words: Aflatoxin M1, raw milk, pasteurized milk, Khoramabad, HPLC
Garro, C J; Mian, L; Cobos Roldán, M
The purpose of this study was to estimate the prevalence of Subclinical ketosis (SCK) between 4 and 19 days in milk (DIM) in a grazing production system and investigate the importance of potential risk factors for SCK. This cross-sectional study was conducted in dairy cows (n = 107), which had more of two parities. The concentration of β-hydroxybutyric (BHB) in blood was quantified through a hand-held meter. Potential risk factors evaluated were calving interval (CI), milk yield in previous lactation, metritis, dystocia, calf sex (male), parity (≤3 vs. ≥4) and pre-partum body condition score (BCS ≤ 3.5 vs. ≥3.75). Prevalence of SCK was 10.3% (95% CI 4.7-15) between 4 and 19 DIM. Risk factors identified were the occurrence of both metritis and pre-partum BCS ≥ 3.75. Cows with metritis had 4.9 (95% CI 1.17-20.98) times more risk of developing SCK than cows without metritis. And the cows with pre-partum BCS ≥ 3.75 had 5.25 (95% CI 1.32-21.11) times more risk of developing SCK than cows with pre-partum BCS ≤ 3.5. Metritis could induce a lower feed intake and promote the development of SCK. High pre-partum BCS could induce a greater mobilization of body reserves altering liver function and aggravating post-partum NEB. The results are indicative of the expected prevalence of SCK in grazing production system. Factors associated could help to identify cattle at risk of SCK and improve the management of strategies to limit the effects. Journal of Animal Physiology and Animal Nutrition © 2013 Blackwell Verlag GmbH.
De Rensis, F; Garcia-Ispierto, I; López-Gatius, F
Heat stress has consequences on both the physiology and reproductive performance of cows, but the most dramatic effect for dairy producers is the decrease produced in fertility. The effects of heat stress on fertility include an increased number of days open, reduced conception rate, and larger number of cows suffering different types of anestrus. Once becomes pregnant, heat stress affects also the reproductive success of the cow through its direct effects on the ovary, uterus, gametes, embryo, and early fetus. This article reviews current knowledge of the effects of heat stress on fertility in dairy cows and the hormonal strategies used to mitigate these effects at the farm level. Administration of GnRH at the moment of artificial insemination can improve the conception rate. Breeding synchronization protocols for fixed-time insemination may reduce the calving conception interval and the number of services per conception. Progesterone-based protocols seem resolve better the reproductive disorders related to a hot environment (anestrus) than GnRH-based protocols. The use of combinations of GnRH, eCG, and hCG in progesterone-based protocols can improve results. Progesterone supplementation during the late embryonic and/or early fetal period would be useful in curtailing pregnancy losses, mainly in single pregnancies, whereas a more positive effect of treatment with GnRH than progesterone has been found in twin pregnancies. Melatonin therapy is emerging as a promising strategy to improve the natural reproductive performance of cows suffering conditions of heat stress. Copyright © 2015 Elsevier Inc. All rights reserved.
Keywords: Cow hide consumption, cow hide value chain, ponmo .... value chain;. 2. identify sources of information of the respondents on the proposed ban; ..... the health risk associated with the consumption of ponmo may favour its proposed.
to put maximum efforts to improve routine management activities to maintain performance evaluated animals .... Cows with repeated heat may be served up to four times. Cows not ..... sire selection and to maximize production and productivity.
Khairkar, Praveen; Tiple, Prashant; Bang, Govind
Although abuse of several unusual inhalants had been documented, addiction to cow dung fumes or their ashes has not been reported in medical literature as yet. We are reporting a case of cow dung dependence in ingestion and inhalational form.
Sep 5, 2011 ... supplementation in multiparous dairy cows diet may improve their milk yield in transition period. Key words: Dairy cow, ... 20 multiparous Holsteins (parity 3) housed in free stalls at the. Esfahan-Kesht farm .... Corn gluten meal.
Evaluation of fertility traits of Friesian X Bunaji dairy cows. ... days open (DO) , number of insemination per conception (NIC), and non- return rate 56 days ... Keywords: Fertility, Friesian x Bunaji cows, Parity, Body condition score, Season, Year ...
Steeneveld, W.; Werven, van T.; Barkema, H.W.; Hogeveen, H.
Under Dutch circumstances, most clinical mastitis (CM) cases of cows on dairy farms are treated with a standard intramammary antimicrobial treatment. Several antimicrobial treatments are available for CM, differing in antimicrobial compound, route of application, duration, and cost. Because cow
Economics of Local Cow Milk Products Marketing in Kwara State, Nigeria. ... The marketing chain for the commodity is simple and crude. It starts from the raw cow milk processors ... EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL ...
Full Text Available Physiology, is the fundamental and functional expression of life. It is the study of all the representative functions of Man in all his capacities, and in particular, his capacity to work. It is very possible to establish a link between a physiological and physiopathological state, the capacity of work and the economy, which can be understood as the articulation between the physiological capacities of Man and the production of work. If these functions are innately acquired by Man they are likewise maintained by regulatory functions throughout life. The stability of these regulatory mechanisms represent the state of good health. The management of this state, constitutes Primary Prevention where both chronic and acute physiopathology defines an alteration in these regulatory mechanisms. We deduce from this reasoning that a tripartite management adapted to the physiological situation is viable and that by choosing parameters specific to individual and collective behavior, it is possible to inject, and combine, at each level and to each demand in order to budget a healthcare system in a more balanced and equitable way.
Proximate analyses carried out on samples of cow milk, soymilk and cow/soymilk Yoghurts showed significant difference (P<0.05) in the % crude protein, crude fat and carbohydrate contents. For crude protein they were 3.49 ± 0.04, 2.78 ± 0.13 and 2.02 ± 0.4 for cow yoghurt, cow/soy yoghurt and soy yoghurt respectively.
Heine, Ralf G; AlRefaee, Fawaz; Bachina, Prashant; De Leon, Julie C; Geng, Lanlan; Gong, Sitang; Madrazo, José Armando; Ngamphaiboon, Jarungchit; Ong, Christina; Rogacion, Jossie M
Lactose is the main carbohydrate in human and mammalian milk. Lactose requires enzymatic hydrolysis by lactase into D-glucose and D-galactose before it can be absorbed. Term infants express sufficient lactase to digest about one liter of breast milk daily. Physiological lactose malabsorption in infancy confers beneficial prebiotic effects, including the establishment of Bifidobacterium-rich fecal microbiota. In many populations, lactase levels decline after weaning (lactase non-persistence; LNP). LNP affects about 70% of the world's population and is the physiological basis for primary lactose intolerance (LI). Persistence of lactase beyond infancy is linked to several single nucleotide polymorphisms in the lactase gene promoter region on chromosome 2. Primary LI generally does not manifest clinically before 5 years of age. LI in young children is typically caused by underlying gut conditions, such as viral gastroenteritis, giardiasis, cow's milk enteropathy, celiac disease or Crohn's disease. Therefore, LI in childhood is mostly transient and improves with resolution of the underlying pathology. There is ongoing confusion between LI and cow's milk allergy (CMA) which still leads to misdiagnosis and inappropriate dietary management. In addition, perceived LI may cause unnecessary milk restriction and adverse nutritional outcomes. The treatment of LI involves the reduction, but not complete elimination, of lactose-containing foods. By contrast, breastfed infants with suspected CMA should undergo a trial of a strict cow's milk protein-free maternal elimination diet. If the infant is not breastfed, an extensively hydrolyzed or amino acid-based formula and strict cow's milk avoidance are the standard treatment for CMA. The majority of infants with CMA can tolerate lactose, except when an enteropathy with secondary lactase deficiency is present.
Coscia, A; Orrù, S; Di Nicola, P; Giuliani, F; Rovelli, I; Peila, C; Martano, C; Chiale, F; Bertino, E
Cow's milk proteins (CMPs) are among the best characterized food allergens. Cow's milk contains more than twenty five different proteins, but only whey proteins alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin (BSA), and lactoferrin, as well as the four caseins, have been identified as allergens. Aim of this study was to investigate by proteomics techniques cow's milk allergens in human colostrum of term and preterm newborns' mothers, not previously detected, in order to understand if such allergens could be cause of sensitization during lactation. Term colostrum samples from 62 healthy mothers and preterm colostrum samples from 11 healthy mothers were collected for this purpose. The most relevant finding was the detection of the intact bovine alpha-S1-casein in both term and preterm colostrum. Using this method, which allows direct proteins identification, beta-lactoglobulin was not detected in any of colostrum samples. According to our results bovine alpha 1 casein that is considered a major cow's milk allergen is readily secreted in human milk: further investigations are needed in order to clarify if alpha-1-casein has a major role in sensitization or tolerance to cow's milk of exclusively breastfed predisposed infants.
Huber, K.; Dänicke, S.; Rehage, J.; Sauerwein, H.; Otto, W.; Rolle-Kampczyk, U.; von Bergen, M.
The failure to adapt metabolism to the homeorhetic demands of lactation is considered as a main factor in reducing the productive life span of dairy cows. The so far defined markers of production performance and metabolic health in dairy cows do not predict the length of productive life span satisfyingly. This study aimed to identify novel pathways and biomarkers related to productive life in dairy cows by means of (targeted) metabolomics. In a longitudinal study from 42 days before up to 100 days after parturition, we identified metabolites such as long-chain acylcarnitines and biogenic amines associated with extended productive life spans. These metabolites are mainly secreted by the liver and depend on the functionality of hepatic mitochondria. The concentrations of biogenic amines and some acylcarnitines differed already before the onset of lactation thus indicating their predictive potential for continuation or early ending of productive life. PMID:27089826
Koch, Franziska; Lamp, Ole; Eslamizad, Mehdi; Weitzel, Joachim; Kuhla, Björn
Climate changes lead to rising temperatures during summer periods and dramatic economic losses in dairy production. Modern high-yielding dairy cows experience severe metabolic stress during the transition period between late gestation and early lactation to meet the high energy and nutrient requirements of the fetus or the mammary gland, and additional thermal stress during this time has adverse implications on metabolism and welfare. The mechanisms enabling metabolic adaptation to heat apart from the decline in feed intake and milk yield are not fully elucidated yet. To distinguish between feed intake and heat stress related effects, German Holstein dairy cows were first kept at thermoneutral conditions at 15°C followed by exposure to heat-stressed (HS) at 28°C or pair-feeding (PF) at 15°C for 6 days; in late-pregnancy and again in early lactation. Liver and muscle biopsies and plasma samples were taken to assess major metabolic pathway regulation using real-time PCR and Western Blot. The results indicate that during heat stress, late pregnant cows activate Cahill but reduce Cori cycling, prevent increase in skeletal muscle fatty acid oxidation, and utilize increased amounts of pyruvate for gluconeogenesis, without altering ureagenesis despite reduced plane of nutrition. These homeorhetic adaptations are employed to reduce endogenous heat production while diverting amino acids to the growing fetus. Metabolic adaptation to heat stress in early lactation involves increased long-chain fatty acid degradation in muscle peroxisomes, allowance for muscle glucose utilization but diminished hepatic use of amino acid-derived pyruvate for gluconeogenesis and reduced peroxisomal fatty acid oxidation and ATP production in liver of HS compared to PF cows in early lactation. Consequently, metabolic adaptation to heat stress and reduced feed intake differ between late pregnancy and early lactation of dairy cows to maintain energy supply for fetus development or milk production
Eslamizad, Mehdi; Weitzel, Joachim; Kuhla, Björn
Climate changes lead to rising temperatures during summer periods and dramatic economic losses in dairy production. Modern high-yielding dairy cows experience severe metabolic stress during the transition period between late gestation and early lactation to meet the high energy and nutrient requirements of the fetus or the mammary gland, and additional thermal stress during this time has adverse implications on metabolism and welfare. The mechanisms enabling metabolic adaptation to heat apart from the decline in feed intake and milk yield are not fully elucidated yet. To distinguish between feed intake and heat stress related effects, German Holstein dairy cows were first kept at thermoneutral conditions at 15°C followed by exposure to heat-stressed (HS) at 28°C or pair-feeding (PF) at 15°C for 6 days; in late-pregnancy and again in early lactation. Liver and muscle biopsies and plasma samples were taken to assess major metabolic pathway regulation using real-time PCR and Western Blot. The results indicate that during heat stress, late pregnant cows activate Cahill but reduce Cori cycling, prevent increase in skeletal muscle fatty acid oxidation, and utilize increased amounts of pyruvate for gluconeogenesis, without altering ureagenesis despite reduced plane of nutrition. These homeorhetic adaptations are employed to reduce endogenous heat production while diverting amino acids to the growing fetus. Metabolic adaptation to heat stress in early lactation involves increased long-chain fatty acid degradation in muscle peroxisomes, allowance for muscle glucose utilization but diminished hepatic use of amino acid-derived pyruvate for gluconeogenesis and reduced peroxisomal fatty acid oxidation and ATP production in liver of HS compared to PF cows in early lactation. Consequently, metabolic adaptation to heat stress and reduced feed intake differ between late pregnancy and early lactation of dairy cows to maintain energy supply for fetus development or milk production
Cows have been a favorite subject for many artists, including Canadian artist Joe Fafard. In this article, grade 11 graphic-design students do a series of exercises in their sketchbooks using the cow motif. Each exercise was designed to have students move from traditional pictures of the dairy cow to more eclectic visual solutions. Eight…
Kneepkens, C. M. Frank; Meijer, Yolanda
Introduction Cow's milk allergy (CMA) is thought to affect 2-3% of infants. The signs and symptoms are nonspecific and may be difficult to objectify, and as the diagnosis requires cow's milk elimination followed by challenge, often, children are considered cow's milk allergic without proven
Studies have been carried out on the production of biogas from mixture of cow abdominal waste and its dung. The rate of biogas production and cumulative volume of the gas produced was compared with that of pure cow dung under the same experimental conditions. The result shows that the mixture of the cow abdominal ...
Jónsson, Ragnar Ingi; Björgvinssin, Trausti; Blanke, Mogens
This paper addresses detection of oestrus in dairy cows using methods from statistical change detection. The activity of the cows was measured by a necklace attached sensor. Statistical properties of the activity measure were investigated. Using data sets from 17 cows, diurnal activity variations...
Mol, de R.M.
Detection models for oestrus and mastitis in dairy cows were developed, based on sensors for milk yield, milk temperature, electrical conductivity of milk, cow's activity and concentrate intake, and on combined processing of the sensor data. The detection model generated alerts for cows,
The results of behavioural studies of dairy cows' behaviour under some modern housing and management conditions are presented. Social dominance in dairy cows is studied and methods to describe social dominance are discussed. The lying behaviour of dairy cows is studied under various
... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Physical examination of cows. 1210.12 Section 1210... FEDERAL IMPORT MILK ACT Inspection and Testing § 1210.12 Physical examination of cows. (a) Physical examination of any and all cows in herds producing milk or cream which is to be shipped or transported into...
Tan, Eng Kien; Tan, Eng Loy
Pregnant women undergo profound anatomical and physiological changes so that they can cope with the increased physical and metabolic demands of their pregnancies. The cardiovascular, respiratory, haematological, renal, gastrointestinal and endocrine systems all undergo important physiological alterations and adaptations needed to allow development of the fetus and to allow the mother and fetus to survive the demands of childbirth. Such alterations in anatomy and physiology may cause difficulties in interpreting signs, symptoms, and biochemical investigations, making the clinical assessment of a pregnant woman inevitably confusing but challenging. Understanding these changes is important for every practicing obstetrician, as the pathological deviations from the normal physiological alterations may not be clear-cut until an adverse outcome has resulted. Only with a sound knowledge of the physiology and anatomy changes can the care of an obstetric parturient be safely optimized for a better maternal and fetal outcome. Copyright © 2013 Elsevier Ltd. All rights reserved.
Vieira, F V R; Cooke, R F; Aboin, A C; Lima, P; Vasconcelos, J L M
The objective of this experiment was to evaluate the effects of glucose infusion on serum concentrations of glucose, insulin, and progesterone (P4), as well as mRNA expression of hepatic CYP2C19 and CYP3A4 in nonlactating, ovariectomized cows in adequate nutritional status. Eight Gir × Holstein cows were maintained on a low-quality Brachiaria brizantha pasture with reduced forage availability, but they individually received, on average, 3 kg/cow daily (as fed) of a corn-based concentrate from d -28 to 0 of the experiment. All cows had an intravaginal P4-releasing device inserted on d -14, which remained in cows until the end of the experiment (d 1). On d 0, cows were randomly assigned to receive, in a crossover design containing 2 periods of 24h each (d 0 and 1), (1) an intravenous glucose infusion (GLUC; 0.5 g of glucose/kg of BW, over a 3-h period) or (2) an intravenous saline infusion (SAL; 0.9%, over a 3-h period). Cows were fasted for 12h before infusions, and they remained fasted during infusion and sample collections. Blood samples were collected at 0, 3, and 6h relative to the beginning of infusions. Liver biopsies were performed concurrently with blood collections at 0 and 3h. After the last blood collection of period 1, cows received concentrate and returned to pasture. Cows gained BW (16.5 ± 3.6 kg) and BCS (0.08 ± 0.06) from d -28 to 0. Cows receiving GLUC had greater serum glucose and insulin concentrations at 3h compared with SAL cohorts. No treatment effects were detected for serum P4 concentrations, although mRNA expression of CYP2C19 and CYP3A4 after the infusion period was reduced for cows in the GLUC treatment compared with their cohorts in the SAL treatment. In conclusion, hepatic CYP3A4 and CYP2C19 mRNA expression can be promptly modulated by glucose infusion followed by acute increases in circulating insulin, which provides novel insight into the physiological mechanisms associating nutrition and reproductive function in dairy cows
Zapata, Rizaldy C; Salehi, Reza; Ambrose, Divakar J; Chelikani, Prasanth K
Dietary fat supplementation during the periparturient period is one strategy to increase energy intake and attenuate the degree of negative energy balance during early lactation; however, little is known of the underlying hormonal and metabolic adaptations. We evaluated the effects of prepartum fat supplementation on energy-balance parameters and plasma concentrations of glucagon-like peptide-1, peptide tyrosine-tyrosine (PYY), adropin, insulin, leptin, glucose, nonesterified fatty acid, and β-hydroxybutyric acid in dairy cows. Twenty-four pregnant dairy cows were randomized to diets containing either rolled canola or sunflower seed at 8% of dry matter, or no oilseed supplementation, during the last 5 wk of gestation and then assigned to a common lactation diet postpartum. Blood samples were collected at -2, +2, and +14 h relative to feeding, at 2 wk after the initiation of the diets, and at 2 wk postpartum. Dietary canola and sunflower supplementation alone did not affect energy balance, body weight, and plasma concentrations of glucagon-like peptide-1, PYY, adropin, insulin, leptin, nonesterified fatty acid, and β-hydroxybutyric acid; however, canola decreased and sunflower tended to decrease dry matter intake. We also observed that the physiological stage had a significant, but divergent, effect on circulating hormones and metabolite concentrations. Plasma glucagon-like peptide-1, PYY, adropin, nonesterified fatty acid, and β-hydroxybutyric acid concentrations were greater postpartum than prepartum, whereas glucose, insulin, leptin, body weight, and energy balance were greater prepartum than postpartum. Furthermore, the interaction of treatment and stage was significant for leptin and adropin, and tended toward significance for PYY and insulin; only insulin exhibited an apparent postprandial increase. Postpartum PYY concentrations exhibited a strong negative correlation with body weight, suggesting that PYY may be associated with body weight regulation during
Adaptive optics is a technology enhancing the visual performance of an optical system by correcting its optical aberrations. Adaptive optics have already enabled several breakthroughs in the field of visual sciences, such as improvement of visual acuity in normal and diseased eyes beyond physiologic limits, and the correction of presbyopia. Adaptive optics technology also provides high-resolution, in vivo imaging of the retina that may eventually help to detect the onset of retinal conditions at an early stage and provide better assessment of treatment efficacy. Copyright © 2016 Elsevier Masson SAS. All rights reserved.
Effects of milk containing only A2 beta casein versus milk containing both A1 and A2 beta casein proteins on gastrointestinal physiology, symptoms of discomfort, and cognitive behavior of people with self-reported intolerance to traditional cows' milk.
Jianqin, Sun; Leiming, Xu; Lu, Xia; Yelland, Gregory W; Ni, Jiayi; Clarke, Andrew J
Cows' milk generally contains two types of β-casein, A1 and A2 types. Digestion of A1 type can yield the peptide β-casomorphin-7, which is implicated in adverse gastrointestinal effects of milk consumption, some of which resemble those in lactose intolerance. This study aimed to compare the effects of milk containing A1 β-casein with those of milk containing only A2 β-casein on inflammation, symptoms of post-dairy digestive discomfort (PD3), and cognitive processing in subjects with self-reported lactose intolerance. Forty-five Han Chinese subjects participated in this double-blind, randomized, 2 × 2 crossover trial and consumed milk containing both β-casein types or milk containing only A2 β-casein. Each treatment period was 14 days with a 14-day washout period at baseline and between treatment periods. Outcomes included PD3, gastrointestinal function (measured by smart pill), Subtle Cognitive Impairment Test (SCIT), serum/fecal laboratory biomarkers, and adverse events. Compared with milk containing only A2 β-casein, the consumption of milk containing both β-casein types was associated with significantly greater PD3 symptoms; higher concentrations of inflammation-related biomarkers and β-casomorphin-7; longer gastrointestinal transit times and lower levels of short-chain fatty acids; and increased response time and error rate on the SCIT. Consumption of milk containing both β-casein types was associated with worsening of PD3 symptoms relative to baseline in lactose tolerant and lactose intolerant subjects. Consumption of milk containing only A2 β-casein did not aggravate PD3 symptoms relative to baseline (i.e., after washout of dairy products) in lactose tolerant and intolerant subjects. Consumption of milk containing A1 β-casein was associated with increased gastrointestinal inflammation, worsening of PD3 symptoms, delayed transit, and decreased cognitive processing speed and accuracy. Because elimination of A1 β-casein attenuated these effects
Galston, A. W.; Sawhney, R. K.
The diamine putrescine, the triamine spermidine, and the tetramine spermine are ubiquitous in plant cells, while other polyamines are of more limited occurrence. Their chemistry and pathways of biosynthesis and metabolism are well characterized. They occur in the free form as cations, but are often conjugated to small molecules like phenolic acids and also to various macromolecules. Their titer varies from approximately micromolar to more than millimolar, and depends greatly on environmental conditions, especially stress. In cereals, the activity of one of the major polyamine biosynthetic enzymes, arginine decarboxylase, is rapidly and dramatically increased by almost every studied external stress, leading to 50-fold or greater increases in putrescine titer within a few hours. The physiological significance of this increase is not yet clear, although most recent work suggests an adaptive, protective role. Polyamines produced through the action of ornithine decarboxylase, by contrast, seem essential for DNA replication and cell division. The application of exogenous polyamines produces effects on patterns of senescence and morphogenesis, suggesting but not proving a regulatory role for polyamines in these processes. The evidence for such a regulatory role is growing.
Armengol, Ramon; Fraile, Lorenzo
Acute puerperal metritis (APM) and clinical metritis (CM) are uterine diseases frequently diagnosed in dairy cows. These diseases are responsible for important economic loss because of their effect not only on reproductive performance but also on milk production. The objective of this study was to assess the impact of two different treatments for metritis on dairy cows by measuring their reproductive performance in the next gestation. The end points to measure the reproductive performance included the conception rate at the first artificial insemination, the number of days at conception, and the proportion of nonpregnant cows at over 150 days after beginning milk production. The study was carried out in a high production dairy cow farm located in Lleida (northeast Spain). Recordings of 1044 parturitions of 747 Holstein cows were controlled in this farm from 2009 to 2014. Cows were diagnosed as suffering from metritis (APM or CM) if the following parameters were observed: an abnormally enlarged uterus; a fetid, watery, reddish brown uterine discharge with (APM) or without (CM) fever (>39.5 °C); and presence (APM) or absence (CM) of signs of systemic illness (decreased milk production, dullness, or other signs of toxemia) within 21 days postpartum. Afterwards, cows suffering from metritis (APM or CM) were randomly assigned and balanced to two groups: (1) animals receiving parenteral amoxicillin intramuscularly plus intrauterine infusion with oxytetracycline (P + I group) and (2) animals receiving only parenteral amoxicillin intramuscularly (P group). Furthermore, reproductive performance of cows without metritis was used as reference (control group). Metritis was diagnosed in 27.5% of the total parturitions included in the study (288 of 1044). In particular, metritis was diagnosed in 30.5% (118 of 387) and 25.9% (170 of 657) of parturitions from heifers and multiparous cows, respectively. Reproductive performance was not significantly affected by the parity, the
OBJECTIVE: The primary objective of this review is to discuss the clinical features, diagnosis, natural history, and prognosis of cow's milk allergy in early childhood and its relationship to development of inhalant allergies. DATA SOURCES: A review of 229 PubMed (National Library of Medicine......) articles on cow's milk allergy (CMPA) for the years 1967 through 2001 was performed. In addition, references from other review articles have been included. This review represents a synthesis of these sources and the expert opinion of the author. STUDY SELECTION: The expert opinion of the author was used...... to select the relevant data for this review. RESULTS: The diagnosis of reproducible adverse reactions to cow's milk protein (CMP), ie, CMPA, has to be confirmed by controlled elimination and challenge procedures. The incidence of CMPA in infancy seems to be approximately 2 to 3% in developed countries...
Lauwere, de C.C.K.; Hendriks, M.M.B.; Metz, J.H.M.; Schouten, W.G.P.
The introduction of fully automatic milking systems (AMS) on dairy farms can only succeed if cows visit the system more or less voluntarily, at regular intervals. This can be arranged by either forced or free cow traffic. In the case of forced cow traffic, the AMS is the only route from the lying
Reducing the amount of supplemental feed postpartum without affecting productivity may enhance profitability of cow-calf operations. Therefore, sixteen 2-yr-old fall calving cows were used to evaluate effects of delaying postpartum supplementation on milk production, serum metabolites, cow and calf ...
Denis, M; Loras-Duclaux, I; Lachaux, A
Cow's milk protein allergy (CMPA) is the first allergy that affects infants. In this population, the incidence rate reaches 7.5%. The multiplicity and aspecificity of the symptoms makes its diagnosis sometimes complicated, especially in the delayed type (gastrointestinal, dermatological, and cutaneous). CMPA symptoms can develop in exclusively breastfed infants with an incidence rate of 0.5%. It, therefore, raises questions about sensitization to cow's milk proteins through breast milk. Transfer of native bovine proteins such as β-lactoglobulin into the breast milk is controversial: some authors have found bovine proteins in human milk but others point to cross-reactivity between human milk proteins and cow's milk proteins. However, it seems that a small percentage of dietary proteins can resist digestion and become potentially allergenic. Moreover, some authors suspect the transfer of some of these dietary proteins from the maternal bloodstream to breast milk, but the mechanisms governing sensitization are still being studied. Theoretically, CMPA diagnosis is based on clinical observations, prick-test or patch-test results, and cow's milk-specific IgE antibody concentration. A positive food challenge test usually confirms the diagnosis. No laboratory test is available to make a certain diagnosis, but the detection of eosinophil cationic protein (ECP) in the mother's milk, for example, seems to be advantageous since it is linked to CMA. Excluding cow's milk from the mother's diet is the only cure when she still wants to breastfeed. Usually, cow's milk proteins are reintroduced after 6 months of exclusion. Indeed, the prognosis for infants is very good: 80% acquire a tolerance before the age of 3 or 4 years. Mothers should not avoid dairy products during pregnancy and breastfeeding as preventive measures against allergy. Copyright Â© 2011 Elsevier Masson SAS. All rights reserved.
Zhigang Zhang, Guowen Liu1, Hongbin Wang, Xiaobing Li1 and Zhe Wang1*
Full Text Available Ketosis is a common metabolic disorder frequently observed in dairy cows during the early lactation period. It is characterized by increased levels of ketone bodies in the blood, urine, and milk. Subclinical ketosis (SCK in dairy cattle is an excess level of circulating ketone bodies in the absence of clinical signs of ketosis. Usually, detection of SCK is carried out by testing the ketone concentrations in blood, urine, and milk. Here, This review overview the detection methods for SCK in dairy cows, including cowside and laboratory tests.
Elisa Junqueira Oliveira
Full Text Available The milk yield has been the most selected trait in dairy cattle breeding programs. However various studies have shown a decline in adaptive and longevity traits in herds that are under selection for improving production, especially in taurine breeds, as the Holstein, who was highly selected for milk production. The aim of this study was to estimate genetic parameters for first lactation 305-day milk yield (Y305 and for longevity traits and to verify the association among them, in high production Holstein cows. The data sets used were from Agrindus Farm, with calving occurring between 1989 and 2005. The traits analyzed were Y305, productive life (PL, calculated as the length of lactation days from the first day of lactation until the culling, and age at culling (AC. Variance components were estimated by Restricted Maximum Likelihood, applying multi-trait animal model. Heritability estimates for Y305, PL and AC were, respectively, 0.35, 0.07 and 0.10. Heritability estimates for PL and AC suggest small genetic variability to get genetic gains by direct selection for these traits, because they are influenced by decisions of voluntary and involuntary culling, being largely affected by factors related to the environment. It is difficult to measure these traits because it is necessary to evaluate culling of animals and causes of culling. The magnitude of the heritability estimate for Y305 evidences the existence of reasonable additive genetic variability, which allows efficiency by selecting for this trait. The genetic correlations between Y305 and PL was 0.02 and between Y305 and AC was 0.01, suggesting small genetic association between Y305 and longevity traits. In this case, the selection for Y305 is viable due to high heritability estimate and the favorable and almost null genetic correlation between Y305 and the longevity traits. Some studies have used to analyze longevity traits as threshold and since this trait has great economic importance, it
Bridgham, Jamie T.; Kelly, Scott A.; Garland, Theodore
Whole animal physiological performance is highly polygenic and highly plastic, and the same is generally true for the many subordinate traits that underlie performance capacities. Quantitative genetics, therefore, provides an appropriate framework for the analysis of physiological phenotypes and can be used to infer the microevolutionary processes that have shaped patterns of trait variation within and among species. In cases where specific genes are known to contribute to variation in physiological traits, analyses of intraspecific polymorphism and interspecific divergence can reveal molecular mechanisms of functional evolution and can provide insights into the possible adaptive significance of observed sequence changes. In this review, we explain how the tools and theory of quantitative genetics, population genetics, and molecular evolution can inform our understanding of mechanism and process in physiological evolution. For example, lab-based studies of polygenic inheritance can be integrated with field-based studies of trait variation and survivorship to measure selection in the wild, thereby providing direct insights into the adaptive significance of physiological variation. Analyses of quantitative genetic variation in selection experiments can be used to probe interrelationships among traits and the genetic basis of physiological trade-offs and constraints. We review approaches for characterizing the genetic architecture of physiological traits, including linkage mapping and association mapping, and systems approaches for dissecting intermediary steps in the chain of causation between genotype and phenotype. We also discuss the promise and limitations of population genomic approaches for inferring adaptation at specific loci. We end by highlighting the role of organismal physiology in the functional synthesis of evolutionary biology. PMID:26041111
Yanagida, Noriyuki; Minoura, Takanori; Kitaoka, Setsuko
We performed an oral food challenge (OFC) with 10 g of butter (equivalent of 2.9 mL cow's milk) and 25-mL heated cow's milk for 68 children with cow's milk-allergy. Thirty-eight children reacted only to heated cow's milk. Twenty-four children reacted to neither heated milk nor butter. Thirty-eight (86.4%) of 44 patients with positive results to the OFC for heated milk could safely tolerate butter. It is highly likely that even children with cow's milk-allergy who show positive results to an O...
Haque, M.A.; Rashid, M.H.; Kajal, M.F.I.; Istiak, M.S.
This experiment was conducted to study quality of Chamcham manufactured from cow milk and to compare it with Chamcham manufactured from buffalo milk and mixture of cow and buffalo milk. Three types of Chamcham were prepared from cow milk(A), buffalo milk(B) and 50% cow +50% buffalo milk(C).In this experiment the quality of prepared Chamcham were evaluated with the help of chemical test. The moisture, total solids, protein, fat, ash and carbohydrate contents of cow milk and buffalo milk Chamch...
Full Text Available The milk freezing point depression (FPD is important physical property. FPD is influenced by milk composition especially by components with osmotic pressure activity and by other physiological factors. There is possible to indicate a foreign (extraneous water addition into milk by FPD. This is necessary to have a good estimated legislative FPD discrimination limit (FPD–L for purpose of milk quality control. This paper was aimed at obtaining information to improve such estimation. Impacts factors as season variations, estimated state of dairy cow nutrition and some milk components and properties on milk FPD and their relations to FPD were quantified (n 11 540 – 72 607 bulk raw cow milk samples. The highest FPD was in Spring (−0.52097 ± 0.004877 °C, the lowest in Autumn (−0.52516 ± 0.005725 °C; P < 0.001. Correlation between FPD and lactose was 0.35 (P < 0.001. 12% and 5.4% of FPD variability is explainable by lactose and casein variability. Relationship between FPD and urea (U was 0.26 (P < 0.001 in March. The worst FPD was in group with presupposed (according to milk urea and protein combination nitrogen matter (NM and energy (E insufficiency (−0.51855 ± 0.007288 °C. The best FPD was in group with presupposed NM and E surplus in feeding ration (−0.52536 ± 0.004785 °C; P < 0.001. The FPD was worse in suspicion on E deficiency (on the basis of fat/crude protein ratio as compared to presumption for balanced E nourishment of dairy herds (−0.52105 ± 0.006436 °C > −0.52244 ± 0.005367 °C; P < 0.001. Results can improve the estimation of objective FPD–L.
Abdulkader, Fernando; Azevedo-Martins, Anna Karenina; de Arcisio Miranda, Manoel; Brunaldi, Kellen
An important challenge for both students and teachers of physiology is to integrate the differentareas in which physiological knowledge is didactically divided. In developing countries, such an issue is even more demanding, because budget restrictions often affect the physiology program with laboratory classes being the first on the list when it…
Heuvelink, E.; Kierkels, T.
Since 2004 Ep Heuvelink and Tijs Kierkels have been writing a continuing series of plant physiology articles for the Dutch horticultural journal Onder Glas and the international edition In Greenhouses. The book Plant Physiology in Greenhouses consists of 50 of their plant physiology articles. The
Mercadante, P M; Ribeiro, E S; Risco, C; Ealy, A D
Pregnancy-associated glycoproteins (PAG) are produced by the ruminant placenta and secreted into the maternal circulation throughout pregnancy. The extent to which circulating PAG concentrations predict pregnancy outcomes was examined herein. Also, associations between circulating PAG concentrations and various production parameters and clinical diseases were evaluated. Lactating primiparous and multiparous Holstein cows (n=345) were bred via timed artificial insemination using a standard Ovsynch protocol. Pregnancy was diagnosed by transrectal ultrasonography at d 32, 46, and 74 of gestation. Blood was harvested at d 32 to determine plasma concentrations of PAG and progesterone. Cows pregnant at d 32 that subsequently lost their pregnancy at d 46 and 74 had reduced PAG concentrations. Both artificial insemination service number and parity were associated with plasma PAG concentrations. Concentration of PAG in plasma was greater for cows pregnant from their second or later breeding than those pregnant from the first breeding postpartum, and was increased for primiparous compared with multiparous. In addition, cows with greater milk yield had increased plasma PAG concentrations. No association was detected between body condition score and plasma PAG concentrations. Cows that experienced clinical metritis, metabolic problems, or left displacement abomasum in the early postpartum period preceding breeding had greater plasma PAG concentrations than cows not experiencing these clinical diseases. Also, cows with multiple clinical diseases had increased odds of pregnancy loss when compared with cows not experiencing clinical diseases. Odds ratio testing detected a tendency in the relationship between reduced milk yield and increased pregnancy loss. Collectively, these associations illustrate one feature of the early developing placenta that may predict pregnancy outcomes in dairy cattle. It is unclear if plasma PAG are actively involved with mediating pregnancy outcomes
Kirkeby, Carsten Thure; Gussmann, Maya Katrin; Græsbøll, Kaare
Introduction. Mastitis, or intramammary infection (IMI), is one of the most significant diseases in dairy herds worldwide. It is caused by environmental and contagious bacteria. Simulation models have proven useful for evaluating the effect of different control strategies. However, previous...... published models are not cow-specific and therefore not so detailed in the simulation of host-pathogen interactions. If a simulation model is to be used by dairy farmers as a decision-making tool, it needs to be cow-specific because daily management decisions are made on cow level. Furthermore, as IMI......, contagious or mixed), the model should be able to reflect this diversity. Our objective was thus to create a pathogen-, cow- and herd-specific bio-economic simulation model that could simulate multiple pathogens and strains at the same time within a dairy herd. This model should be able to simulate realistic...
1 Agricultural Business Research Institute, UNE Armidale, NSW 2351, Australia .... Table 1 provides summary statistics of cow age and weight at calving and weaning .... Genetic evaluation for the beef industry in. Australia. Aust. J. Exp. Agric.
Full Text Available Ketosis is a metabolic disorder closely associated with liver lipidosis. Numerous tests have been developed to detect hepatic dysfunction in dairy cows. Bromosulfophthalein (BSP clearance is established as a sensitive index of hepatic function. The objective of this study was to examine the difference of biliary excretion of BSP between ketotic and healthy Holstein cows and to correlate this excretion with other indicators of liver dysfunction. Twenty puerperal Holstein cows divided in two groups (10 cows each were involved in the study. The first group included healthy and the second group ketotic cows. Blood samples were taken 10 days after parturition. Concentrations of total protein, albumin, total bilirubin, Ca, P, total lipids, urea and glucose were determined. Immediately after blood sampling, BSP test was performed. Blood samples were taken 5 and 45 minutes after injection, and the percentage of retained pigment in the sample obtained at minute 45 was calculated. Blood albumin and glucose concentrations were significantly higher in healthy then ketotic cows. Total bilirubin concentration was significantly higher in ketotic than healthy cows. BSP excretion was significantly higher in ketotic compared to healthy cows. There was a significant positive correlation between BSP values and total bilirubin concentrartions in both healthy and ketotic cows and a significant negative correlation between BSP values and glucose concentrartions in both healthy and ketotic cows. In conclusion, biliary clearance of BSP may be used as a reliable method for the detection of hepatic dysfunction associated with clinical symptoms of ketosis in dairy cows.
Full Text Available The effect of quantity of nutrients on rumen fermentation and the level of metabolic markers in blood serum were simultaneously analysed in groups of dairy cows 21 days before and 21 days after parturition with aim to diagnose disorders in milk production in the transition period of dairy cows. Results of analysis of health disorders confirmed the following: low energy concentration in the diet insufficiently saturated with fibrous carbohydrates, followed with rapid change to concentrate type of diet after delivery resulted in insufficient adaptation of the rumen metabolism before and after rapid transition to production feeding rations after calving; the level indicative of acidification of the rumen environment. Investigation of intermediary metabolism confirmed pre- and post-partum lipomobilization, with increased values of NEFA in 68 % and 54 % of animals respectively, with liver load manifestation in 37 % and 69 % of animals, respectively.
Heiser, Axel; McCarthy, Allison; Wedlock, Neil; Meier, Susanne; Kay, Jane; Walker, Caroline; Crookenden, Mallory A; Mitchell, Murray D; Morgan, Stuart; Watkins, Kate; Loor, Juan J; Roche, John R
Peripartum, and especially during the transition period, dairy cows undergo dramatic physiological changes. These coincide with an increased risk of disease during the first 2 wk after calving and have been linked to dairy cows failing to achieve production as well as reproductive targets. Previous evidence suggests that these physiological changes affect the immune system and that transition dairy cows experience some form of reduced immunocompetence. However, almost all of these studies were undertaken in high-production, housed dairy cows. Grazing cows have much lower levels of production and this study aimed to provide clarity whether or not the dysfunctional attributes of the peripartum immune system reported in high production housed cows are evident in these animals. Therefore, cell culture techniques, flow cytometry, and quantitative PCR were applied to analyze the cellular composition of peripheral blood mononuclear cells from transition dairy cows as well as the performance of these cells in an in vitro assay. First, a combination of in vitro stimulation and quantitative PCR for cytokines was validated as a quantifiable immunocompetence assay in 29 cattle and a correlation of quantitative PCR and ELISA demonstrated. Second, the relative number of T helper cells, cytotoxic T cells, B cells, γδ T cells, natural killer cells, and monocytes in peripheral blood was measured, of which B cells and natural killer cells increased in number postcalving (n=29) compared with precalving. Third, following in vitro stimulation cytokine profiles indicated decreased expression of IFNγ, tumor necrosis factor, and IL-17 and increased expression of IL-10 wk 1 after calving, which later all returned to precalving values (n=39). Additionally, treatment of transition cows with a nonsteroidal anti-inflammatory drug (i.e., carprofen) administered on d 1, 3, and 5 postcalving (n=19; untreated control n=20) did not affect the cytokine expression at any time point. In conclusion
Lifschitz, Carlos; Szajewska, Hania
This review summarizes current evidence and recommendations regarding cow?s milk allergy (CMA), the most common food allergy in young children, for the primary and secondary care providers. The diagnostic approach includes performing a medical history, physical examination, diagnostic elimination diets, skin prick tests, specific IgE measurements, and oral food challenges. Strict avoidance of the offending allergen is the only therapeutic option. Oral immunotherapy is being studied, but it is...
Maurer, Michaela; Peinhopf, Walter; Gottschalk, Jutta; Einspanier, Almut; Koeller, Gabor; Wittek, Thomas
In this Research Paper we hypothesised that the temporary insulin resistance seen during the transition period in dairy cows may cause significant differences in the efficacy of PG at different sampling periods and that in some cases this effect will be dose dependent. Eighty four sampling sets were generated by studying 7 multiparous Holstein cows repeatedly at 4 sampling periods of 3 d length (dry cows: days 40, 39 and 38 antepartum; close up cows: days 10, 9 and 8 antepartum; fresh cows: days 3, 4 and 5 post-partum; lactating cows: days 38, 39 and 40 post-partum). On each of these days 3 h after morning feeding propylene glycol was drenched in different dosages of 100, 300 or 500 ml once per day (cross over study). The different doses were applied in an alternating order (Latin square). Blood samples were taken before, every 30 min up to 4 h, after 6 and 12 h after PG application. Following parameters have been measured: insulin, non-esterified fatty acids (NEFA), betahydroxybutyrate (BHB), bilirubin, cholesterol, potassium, aspartate aminotransferase (AST) and glutamate dehydrogenase (GLDH). Revised Quantitative Insulin Sensitivity Check Index (RQUICKI) was calculated. It was found that glucose, insulin, NEFA, BHB, bilirubin and potassium concentrations were influenced differently by the three defined dosages of propylene glycol at four different sampling periods. Whereas RQUICKI, cholesterol, AST and GLDH did not differ between the sampling periods and treatments. The major results of the study are that the effect of PG is dose-dependent and that the effect of PG is depending on the time of application according to calving. It can be concluded that in fresh cows higher dosages are necessary to provoke similar effects in comparison to dry, close up and lactating cows. Although the study did not compare to topdressing of PG from the results it is reasonable to believe that bolus application of a specific PG volume is necessary to provoke the effect.
Lubecke, Victor M; Droitcour, Amy D; Park, Byung-Kwon; Singh, Aditya
Presents a comprehensive description of the theory and practical implementation of Doppler radar-based physiological monitoring. This book includes an overview of current physiological monitoring techniques and explains the fundamental technology used in remote non-contact monitoring methods. Basic radio wave propagation and radar principles are introduced along with the fundamentals of physiological motion and measurement. Specific design and implementation considerations for physiological monitoring radar systems are then discussed in detail. The authors address current research and commercial development of Doppler radar based physiological monitoring for healthcare and other applications.
Ditye, Thomas; Javadi, Amir Homayoun; Carbon, Claus-Christian; Walsh, Vincent
Adaptation is an automatic neural mechanism supporting the optimization of visual processing on the basis of previous experiences. While the short-term effects of adaptation on behaviour and physiology have been studied extensively, perceptual long-term changes associated with adaptation are still poorly understood. Here, we show that the integration of adaptation-dependent long-term shifts in neural function is facilitated by sleep. Perceptual shifts induced by adaptation to a distorted imag...
The objectives of the study were to generate a reliable field data set and analyze it to determine reproductive parameters/indices. A total of 280 and 400 households keeping 158 and 709 cows and heifers in Rwanda and Tanzania respectively were studied. Reproductive events: dates of heat, AI or NS, service number, sire ...
Raun, Birgitte Marie Løvendahl
Corn silages with high propanol concentrations has been suspected to cause reduced feed intake and health problems for dairy cows in the post-pattum transition period. With the increasing use of hetero fermentative inoculants to support corn silage fermentation it is likely that silage concentrat......Corn silages with high propanol concentrations has been suspected to cause reduced feed intake and health problems for dairy cows in the post-pattum transition period. With the increasing use of hetero fermentative inoculants to support corn silage fermentation it is likely that silage...... of alcohols will lead to high alcohol concentrations in peripheral bood for a considerable period. Increased hepatic NEFA uptake in the postpartum transition period may result in even further decreased hepatic capacity for alcohol metabolism making post-partum transition cows especially vulnerable to high...... alcohol intakes. In order to evaluate the impact of alcohol fermentation in corn silages on dairy cow performance, the main purpose of this thesis was first to investigate the concentrations and composition of alcohols in typical field corn silages, and second to study how transition and lactating dairy...
Jan 26, 2017 ... Indexes that are consistent with the econometric definition of efficiency and seek to ... defined as ratios, such as the biological efficiency metric calf weight/cow weight. Dinkel & Brown ..... Biometrics 15, 469-485. Scholtz, M.M.& ...
Juhasz-Kaszanyitzky, E.; Janosi, S.; Somogyi, P.; Dan, A.; Graaf-van Bloois, van der L.; Dulijkeren, van E.; Wagenaar, J.A.
We isolated methicillin-resistant Staphylococcus aureus (MRSA) from cows with subclinical mastitis and from a person who worked with these animals. The bovine and human strains were indistinguishable by phenotyping and genotyping methods and were of a low frequency spa type. To our knowledge, this
Blavy, P.; Derks, M.; Martin, O.
The aim of this study was to gain a better understanding of the variability in shape and features of all progesterone profiles during oestrus cycles in cows, and to create templates for cycle shapes and features as a base for further research. Milk progesterone data from 1418 oestrus cycles, coming...
Hymøller, Lone; Jensen, Søren Krogh
The ruminal fate of the fat-soluble vitamins D and E was studied in dairy cows. Ten to 15 kg of ruminal contents was taken from each cow through a ruminal fistula. A sample was taken out (0-h sample) and the rest of the contents were mixed with 4,360 mg of all-rac-α-tocopheryl acetate (vitamin E;...... a single dose of α-tocopheryl acetate led to the conclusion that oral single dose therapy with all-rac-α-tocopheryl acetate is of limited physiological value....
Klop, G; Hatew, B; Bannink, A; Dijkstra, J
An experiment was conducted to study potential interaction between the effects of feeding nitrate and docosahexaenoic acid (DHA; C22:6 n-3) on enteric CH4 production and performance of lactating dairy cows. Twenty-eight lactating Holstein dairy cows were grouped into 7 blocks of 4 cows. Within blocks, cows were randomly assigned to 1 of 4 treatments: control (CON; urea as alternative nonprotein N source to nitrate), NO3 [21 g of nitrate/kg of dry matter (DM)], DHA (3 g of DHA/kg of DM and urea as alternative nonprotein N source to nitrate), or NO3 + DHA (21 g of nitrate/kg of DM and 3 g of DHA/kg of DM, respectively). Cows were fed a total mixed ration consisting of 21% grass silage, 49% corn silage, and 30% concentrates on a DM basis. Feed additives were included in the concentrates. Cows assigned to a treatment including nitrate were gradually adapted to the treatment dose of nitrate over a period of 21 d during which no DHA was fed. The experimental period lasted 17 d, and CH4 production was measured during the last 5d in climate respiration chambers. Cows produced on average 363, 263, 369, and 298 g of CH4/d on CON, NO3, DHA, and NO3 + DHA treatments, respectively, and a tendency for a nitrate × DHA interaction effect was found where the CH4-mitigating effect of nitrate decreased when combined with DHA. This tendency was not obtained for CH4 production relative to dry matter intake (DMI) or to fat- and protein corrected milk (FPCM). The NO3 treatment decreased CH4 production irrespective of the unit in which it was expressed, whereas DHA did not affect CH4 production per kilogram of DMI, but resulted in a higher CH4 production per kilogram of fat- and protein-corrected milk (FPCM) production. The FPCM production (27.9, 24.7, 24.2, and 23. 8 kg/d for CON, NO3, DHA, and NO3 + DHA, respectively) was lower for DHA-fed cows because of decreased milk fat concentration. The proportion of saturated fatty acids in milk fat was decreased by DHA, and the proportion of
O. M. Chernenko
Full Text Available The article presents the results of studying cortisol concentration and creatine phosphokinase activity in the blood serum of 40 half-sib Ukrainian black and white dairy cows an hour after planned blood extraction, which was a stress experience. We divided the cows into three groups according to the distribution of cortisol concentration in the animals: I – high, II – average and III – low stress resistance. During the research, the cows from these groups were in their fourth month of lactation after their first calving. They were kept untethered in their summer quarters, natural pastures. The aim of the research was to identify the differences in the quality of colostrum, the vitality of the calves up to the age of 6 months, the vitality of the mother-cows, and also the influence of their different adaptation potentials in cases of abortion and stillborn calves among the cows from different groups. At the dairy complex where the research was conducted, the quality of the cows’ colostrum is not monitored. This is typical for most dairy plants of Ukraine. We found that the classes of A and M immunoglobulins did not depend upon which particular groups the animals belonged to, whereas the content of immunoglobulins of class G is 6.7 g/l higher, and the content of total protein is 8.9 g/l higher in the yield of colostrum of the cows from group 1. The influence of cows’ different levels of resistance to stress experiences on their incidence of abortions and stillborn calves was not determined. Gastroenteritis occurred among calves of cows from group 3 three times more often than with those of group 1. Vitality of calves from cows of groups 1 and 2 was significantly higher. Therefore, technological stress experiences of mother-cows have a negative effect upon the quality of colostrum and upon the vitality of calves. This factor ought to be taken into consideration in the exploitation of animals, especially during pregnancy in order to
McConnel, C; Lombard, J; Wagner, B; Kopral, C; Garry, F
Summary studies of dairy cow removal indicate increasing levels of mortality over the past several decades. This poses a serious problem for the US dairy industry. The objective of this project was to evaluate associations between facilities, herd management practices, disease occurrence and death rates on US dairy operations through an analysis of the National Animal Health Monitoring System's Dairy 2007 survey. The survey included farms in 17 states that represented 79.5% of US dairy operations and 82.5% of the US dairy cow population. During the first phase of the study operations were randomly selected from a sampling list maintained by the National Agricultural Statistics Service. Only farms that participated in phase I and had 30 or more dairy cows were eligible to participate in phase II. In total, 459 farms had complete data for all selected variables and were included in this analysis. Univariable associations between dairy cow mortality and 162 a priori identified operation-level management practices or characteristics were evaluated. Sixty of the 162 management factors explored in the univariate analysis met initial screening criteria and were further evaluated in a multivariable model exploring more complex relationships. The final weighted, negative binomial regression model included six variables. Based on the incidence rate ratio, this model predicted 32.0% less mortality for operations that vaccinated heifers for at least one of the following: bovine viral diarrhea, infectious bovine rhinotracheitis, parainfluenza 3, bovine respiratory syncytial virus, Haemophilus somnus, leptospirosis, Salmonella, Escherichia coli or clostridia. The final multivariable model also predicted a 27.0% increase in mortality for operations from which a bulk tank milk sample tested ELISA positive for bovine leukosis virus. Additionally, an 18.0% higher mortality was predicted for operations that used necropsies to determine the cause of death for some proportion of dead
Ringseis, R; Gessner, D K; Eder, K
The transition period represents the most critical period in the productive life of high-yielding dairy cows due to both metabolic and inflammatory stimuli, which challenge the liver and predispose dairy cows to develop liver-associated diseases such as fatty liver and ketosis. Despite the fact that all high-yielding dairy cows are affected by marked metabolic stress due to a severe negative energy balance (NEB) during early lactation, not all cows develop liver-associated diseases. Although the reason for this is largely unknown, this indicates that the capacity of the liver to cope with metabolic and inflammatory challenges varies between individual high-yielding dairy cows. Convincing evidence exists that endoplasmic reticulum (ER) stress plays a key role in the development of fatty liver, and it has been recently shown that ER stress occurs in the liver of high-yielding dairy cows. This indicates that ER stress may be involved in the development of liver-associated diseases in dairy cows. The present review shows that the liver of dairy cows during early lactation is exposed to several metabolic and inflammatory challenges, such as non-esterified fatty acids, tumour necrosis factor α, interleukin-1β, reactive oxygen species and lipopolysaccharides, which are known inducers of ER stress. Thus, ER stress may represent a molecular basis for fatty liver development and account for the frequent occurrence of fatty liver and ketosis in high-yielding dairy cows. Interindividual differences between dairy cows in the activation of hepatic stress response pathways, such as nuclear factor E2-related factor 2, which is activated during ER stress and reduces the sensitivity of tissues to oxidative and inflammatory damage, might provide an explanation at the molecular level for differences in the capacity to cope with pathological inflammatory challenges during early lactation and the susceptibility to develop liver-associated diseases between early-lactating dairy cows
Ghavi Hossein-Zadeh, N
The objective of this study was to evaluate the effect of dystocia on the reproductive performance and functional longevity in Iranian Holsteins. Data consisted of 1 467 064 lactation records of 581 421 Holstein cows from 3083 herds which were collected by the Animal Breeding Center of Iran from April 1987 to February 2014. Reproduction traits in this study included interval from first to second calving, days open and days from first calving to first service. The generalized linear model was used for the statistical analysis of reproductive traits. Survival analysis was performed using the Weibull proportional hazards models to analyse the impact of dystocia on functional longevity. The incidence of dystocia had an adverse effect on the reproductive performance of dairy cows. Therefore, reproductive traits deteriorated along with increase in dystocia score (p dystocia (p 33 months had the greatest risk (p dystocia had important negative effects on the reproductive performance and functional longevity in dairy cows, and it should be avoided as much as possible to provide a good perspective in the scope of economic and animal welfare issues in dairy herds. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.
Ş. M. Pancarci
Full Text Available Efficacy of the β-adrenergic receptor blocker (carazolol at Timed Artificial Insemination (TAI was investigated. Cows (n = 73 were pre-synchronized with two PGF2α injections given 14 d apart to initiate the Ovsynch protocol at early and middle luteal stages 14 d later, and received injections of GnRH and PGF2α seven d apart followed by GnRH 48 h later, and TAI 16 - 18 h later. Corpus luteum (CL was detected via rectal palpation at the beginning of the Ovsynch protocol. Carazolol (Treatment I; n = 41 or physiologic saline (Treatment II; n = 32 were administered via jugular vein five min before TAI. Uterine tone was determined prior to infusion and at TAI via rectal palpation. Pregnancies were diagnosed with transrectal ultrasonography 40 ± 7 d after TAI. Uterine tone was 2.8 (1.4 - 5.3 times higher (P P < 0.05, and 66.7%, 75%, 52.6%, 100% and 16.7%, 33.3%, 25%, 28.6% in primiparous cows with CL and without CL, and in multiparous cows with CL and without CL in Treatments I and II, respectively. Pregnancy rates did not differ between treatments I (34.2% and II (40.6%. In conclusion, no beneficial effect of carazolol administration prior to TAI was found except for the increased uterine tone.
Diskin, M G; Kenny, D A
A reproductively efficient beef cow herd will be fundamental to meeting the protein and specifically, red meat demand of an ever increasing global population. However, attaining a high level of reproductive efficiency is underpinned by producers being cognizant of and achieving many key targets throughout the production cycle and requires considerable technical competency. The lifetime productivity of the beef-bred female commences from the onset of puberty and will be dictated by subsequent critical events including age at first calving, duration of the postpartum interval after successive calvings, conception and pregnancy rate, and ultimately manifested as length of intercalving intervals. In calved heifers and mature cows, the onset of ovarian activity, postpartum is a key event dictating the calving interval. Again, this will be the product mainly of prepartum nutrition, manifested through body condition score and the strength of the maternal bond between cow and calf, though there is increasing evidence of a modest genetic influence on this trait. After the initiation of postpartum ovarian cyclicity, conception and subsequent pregnancy rate is generally a function of bull fertility in natural service herds and heat detection and timing of insemination in herds bred through AI. Cows and heifers should be maintained on a steady plane of nutrition during the breeding season, but the contribution of significant excesses or deficiencies of nutrients including protein and trace elements is likely to be minor where adequate pasture is available. Although increased efforts are being made internationally to genetically identify and select for more reproductively efficient beef cows, this is a more long-term strategy and will not replace the need for a high level of technical efficiency and management practice at farm level. Copyright © 2016 Elsevier Inc. All rights reserved.
Full Text Available Currently, the ecological farming is increasingly spread in the European Union. The aim of this relatively young farming method is a friendly approach to agricultural production with an emphasis to deliver healthy raw materials and food to final consumer. Selenium is included in an essential trace micronutrients which are necessary for the proper process of physiological reactions. It is a part of glutathione peroxidase, which is a powerful antioxidant. At present, selenium-deficiency can occur in feed and food in central Europe. Selenium deficiency is one cause of the higher occurrence of cardiovascular diseases. The aim of the experiment was to study whether the addition of selenium to the diet of dairy cows in ecological farming can increase its concentration in milk and affect quantitative (milk yield and quality (content of protein, fat, lactose, somatic cells and urea milk indicators. The experiment included twenty cows of Holstein breed. The first experimental group of cows (n = 10 was fed with selenium in an amount of 0.3 mg.kg-1 (as selenomethionine in the feed dose. The control group (n = 10 was not fed with the increased selenium in the feed dose. The basic feed dose contained 0.17 mg of Se/kg in the diet. For dairy cows, daily intake was of 20.5 kg of dry matter feed. The duration of the experiment was set at 45 days. The selenium concentration in milk was measured from 0.13 to 0.15 µg.mL-1 in the experimental group of cows during the evaluation. The control group of cows without the addition of selenium to the diet showed a selenium concentration below the detection limit. During the experiment, milk yield, lactose, fat and protein were not affected. A significant decrease (p <0.05 of somatic cells by 58% occurred in milk in the experimental group. The amount of urea was significantly lower in both groups in the experimental (by 52%; p <0.05 and control (50%; p <0.05. These results show that the addition of selenium may increase
Full Text Available Abstract Background Mortality risk in Danish dairy cows has more than doubled since 1990 (from 2% in 1990 to 5% in 2005. Until now, registrations about dead cows in the Danish Cattle Database have not included information about whether the cow died unassisted or was euthanized. Methods We interviewed a random sample of 196 Danish dairy farmers that had reported a dead cow to the Danish Cattle Database in 2002 and 196 dairy farmers that had reported a dead cow in 2006. Our objectives were to evaluate the proportion of euthanized cows, changes in the behaviour of farmers regarding euthanasia of cows over the years and possible reasons for these changes. Results It seems that the threshold for euthanasia of cows among farmers has changed. Farmers generally reported a lower threshold for euthanasia compared to 5–10 years ago. Conclusion The threshold for euthanasia of cows has, according to the dairy farmers, become lower. This might have positive impacts on animal welfare as more seriously ill cows are euthanized in the herds and not put through a period of suffering associated with disease and treatment or transported to a slaughterhouse in poor condition.
Ashwinikumar A. Raut
Full Text Available For millennia, cow has been central to Indian economy, life and culture. There are innumerable references-Vedic and subsequent to the sacred significance of cow. The benefits of cow have been described at length in relation to agriculture, environment, health, economy and spiritual progress. However, the socio-political issues surrounding cow as a sacred animal have raised acrimonious debates. In Ayurveda also, there is a long tradition of using cow products for positive health, pharmaceutical processes and in therapeutics. There have been quite a few studies on the activity, efficacy, safety and acceptability of Panchagavya and other cow products. Paradoxically, many cow products available in the market for human consumption require improved standardization and proper regulation.Integration of cowpathy (Govaidyak in traditional Indian systems of medicine has been natural, based on their common dravyagunavigyan. But if its integration with conventional medicine is contemplated, we will need better understanding of the ingredients of cow products, their pharmacokinetics, pharmacodynamics and therapeutic ratio. A rational beginning can be made by data collection of experiential and anecdotal responses. A meticulous analysis of database of panchgavya and other cow products should look for temporal relationships, biological plausibility and translational potential before embarking on state-of-the-art experimental and clinical studies for selected indications. Keywords: Panchgavya, Cow products, Cowpathy, Govaidyak
Balcells, J.; Vicente, F.; Orellana-Boero, P.; Martin-Orue, S.; Gonzalez-Ronquillo, M.
The present study examined the endogenous urinary excretion of purine derivatives (PD: allantoin and uric acid) in normally fed animals in different physiological states. Animals fitted with a simple rumen cannula and a T-shaped duodenal cannula were used in three separate experiments. In Experiment I, three heifers (about 8 months old; 225 ± 4.4 kg) fed a cereal based diet were used. In Experiment 11, two Friesian dry cows (about 24 months old; 696 ± 21 kg) fed at maintenance level on chopped barley straw and barley grain (50:50). In Experiment 3, three multiparous crossbreed Holstein-Friesian cows (560 ± 10 kg, average milk yield 25 ± 3.2 kg/d) in their third lactation were used. The cows were fed a mixed diet (48:52; roughage:concentrate). 15 N ammonium phosphate was infused continuously into the rumen to label microbial purine bases (PB). Duodenal flow of digesta and PB was determined using a dual marker system. After 72-80 h purine enrichment had reached plateau values in body pools and in urine. Daily endogenous PD excretion (μmol/W 0.75 ) obtained in dry cows (310 ± 31.0) were not significantly different from that obtained in growing steers (236 ± 6.0) but were consistently higher (512.4 ± 36.4) in lactating cows. (author)
Mach Casellas, N.; Zom, R.L.G.; Widjaja-Greefkes, H.C.A.; Wikselaar, van P.G.; Weurding, R.E.; Goselink, R.M.A.; Baal, van J.; Smits, M.A.; Vuuren, van A.M.
During the transition period in dairy cows, drastic adaptations within and between key tissues and cell types occur in a coordinated manner to support late gestation, the synthesis of large quantities of milk and metabolic homoeostasis. The start of lactation coincides with an increase of
Metabolites involved in the metabolic adaptation to negative energy balance may have the potential to regulate timing of reproductive success. Therefore, the objective of this 4-yr study was to determine the association of serum metabolites, cow BW, BCS, and calf performance on conception date in 2...
Metabolites involved in the metabolic adaptation to negative energy balance may potentially contribute to regulation of reproductive success. Therefore, the objective of this 4-yr study was to determine the association of serum metabolites, cow BW, BCS, and calf performance on conception date in sp...
During life, the heart is exposed to different types of stresses. In response to changing demands or stimuli the heart can cope by growing in size. In this thesis, molecular changes underlying cardiac physiological and pathological adaptations are investigated. First, we validated physiological
De Matteis, L; Bertoni, G; Lombardelli, R; Wellnitz, O; Van Dorland, H A; Vernay, M C M B; Bruckmaier, R M; Trevisi, E
The link between energy availability, turnover of energy substrates and the onset of inflammation in dairy cows is complex and poorly investigated. To clarify this, plasma inflammatory variables were measured in mid-lactating dairy cows allocated to three groups: hyperinsulinemic hypoglycaemic clamp, induced by insulin infusion (HypoG, n = 5); hyperinsulinemic euglycaemic clamp, induced by insulin and glucose infusion (EuG; n = 6); control, receiving a saline solution infusion (NaCl; n = 6). At 48 h after the start of i.v. infusions, two udder quarters per cow were challenged with 200 μg of E. coli lipopolysaccharide (LPS). Individual blood samples were taken before clamps, before LPS challenge (i.e. 48 h after clamps) and 6.5 h after. At 48 h, positive acute phase proteins (posAPP) did not differ among groups, whereas albumin and cholesterol (index of lipoproteins), negative APP (negAPP), were lower (p insulin at the tissue-level makes dairy cows more susceptible to inflammatory events. In contrast, HypoG seems to attenuate the inflammatory response. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.
Jonsson, N N; Fortes, M R S; Piper, E K; Vankan, D M; de Cisneros, J Prada J; Wittek, T
The periparturient period presents major physiological challenges for the dairy cow. It is a period that is affected by metabolic stressors, major changes in endocrine status, and altered immune function, which together result in an increased risk of disease. Immunological, hematological, and metabolic profiles from the periparturient period of heifers (primipara) were compared with those of cows (pluripara) to test the hypothesis that at the time of calving they have qualitatively different peripheral blood profiles. Blood samples were collected from 22 Holstein-Friesian animals on 3 occasions: approximately 2 wk before calving, within 24h after calving, and approximately 2 wk after calving. Quantitative PCR was used to measure the expression of a selected set of cytokines and receptors by peripheral blood leukocytes. Additional analyses included hemoglobin concentration, red cell, platelet and white cell counts (total and differentiated), and clinical diagnostic biochemical profiles. Total leukocyte counts, neutrophils, and lymphocytes were higher in heifers than cows before calving and within 24h after calving. Alkaline phosphatase was consistently higher in heifers than cows and several significant differences were observed between the 2 groups with regards to cytokine and cytokine-receptor mRNA expression. The results warrant further investigation from the perspective of identifying risk factors for metabolic and parturient disease in dairy cattle. Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Teama, F.E.I.; Gad, A.E.
According to the seasonal variations in environmental conditions in post-partum cows, some biochemical and physiological changes which affect the productive efficiency of farm animals may occur. This study was conducted in the bovine farm of Experimental Farms Project of Nuclear Research Centre, Atomic Energy Authority, Inshas, Egypt, to evaluate some blood biochemical and some hormonal changes during the suckling period in crossbred cows under winter and summer conditions. Alterations in metabolites and metabolic hormones during the first 10 weeks post-partum in both winter and summer during a period of suckling were analyzed on a total of 13 crossbred (Brown Swiss X Balady) cows (winter, n=7; summer, n=6). The blood samples were taken at 2 weeks intervals, 5 times in each season to determine the concentrations and changes in glucose, urea, total cholesterol, total proteins and some hormones including leptin, T4 and progesterone (P4) under winter and summer conditions. The data indicated that total protein (P<0.01), glucose (P<0.05), leptin (P<0.01), total cholesterol (P<0.01), and T4 (P<0.01) had significant seasonal differences between the two calving groups. A positive correlation coefficient was observed between leptin and T4 hormone. From the obtained data, it could be concluded that in summer season, certain biochemical and hormonal levels of calving cows may enhanced but not enough to affect the levels of urea and progesterone. The positive correlation between leptin and T4 may indicate association in the rate of metabolism.
McAssey, M.P.; Helm, J.; Hsieh, F.; Sbarra, D.; Ferrer, E.
A defining feature of many physiological systems is their synchrony and reciprocal influence. An important challenge, however, is how to measure such features. This paper presents two new approaches for identifying synchrony between the physiological signals of individuals in dyads. The approaches are adaptations of two recently-developed techniques, depending on the nature of the physiological time series. For respiration and thoracic impedance, signals that are measured continuously, we use...
Y. Q. Guo
Full Text Available Eight multiparous Holstein cows (569±47 kg of BW; 84±17 DIM were used to evaluate the effects of different levels of coarsely ground wheat (CGW as replacements for ground corn (GC in diets on feed intake and digestion, ruminal fermentation, lactation performance, and plasma metabolites profiles in dairy cows. The cows were settled in a replicated 4×4 Latin square design with 3-wk treatment periods; four cows in one of the replicates were fitted with rumen cannulas. The four diets contained 0, 9.6, 19.2, and 28.8% CGW and 27.9, 19.2, 9.6, and 0% GC on dry matter (DM basis, respectively. Increasing dietary levels of CGW, daily DM intake tended to increase quadratically (p = 0.07; however, apparent digestibility of neutral detergent fiber (NDF and acid detergent fiber (ADF were significantly decreased (p<0.01 in cows fed the 28.8% CGW diets. Ruminal pH remained in the normal physiological range for all dietary treatments at all times, except for the 28.8% CGW diets at 6 h after feeding; moreover, increasing dietary levels of CGW, the daily mean ruminal pH decreased linearly (p = 0.01. Increasing the dietary levels of CGW resulted in a linear increase in ruminal propionate (p<0.01 and ammonia nitrogen (NH3-N (p = 0.06 concentration, while ruminal acetate: propionate decreased linearly (p = 0.03 in cows fed the 28.8% CGW diets. Milk production was not affected by diets; however, percentage and yield of milk fat decreased linearly (p = 0.02 when the level of CGW was increased. With increasing levels of dietary CGW, concentrations of plasma beta-hydroxybutyric acid (BHBA (p = 0.07 and cholesterol (p<0.01 decreased linearly, whereas plasma glucose (p = 0.08, insulin (p = 0.02 and urea nitrogen (p = 0.02 increased linearly at 6 h after the morning feeding. Our results indicate that CGW is a suitable substitute for GC in the diets of dairy cows and that it may be included up to a level of 19.2% of DM without adverse effects on feed intake and digestion
Gorban, Alexander N; Tyukina, Tatiana A; Smirnova, Elena V; Pokidysheva, Lyudmila I
In 1938, Selye proposed the notion of adaptation energy and published 'Experimental evidence supporting the conception of adaptation energy.' Adaptation of an animal to different factors appears as the spending of one resource. Adaptation energy is a hypothetical extensive quantity spent for adaptation. This term causes much debate when one takes it literally, as a physical quantity, i.e. a sort of energy. The controversial points of view impede the systematic use of the notion of adaptation energy despite experimental evidence. Nevertheless, the response to many harmful factors often has general non-specific form and we suggest that the mechanisms of physiological adaptation admit a very general and nonspecific description. We aim to demonstrate that Selye׳s adaptation energy is the cornerstone of the top-down approach to modelling of non-specific adaptation processes. We analyze Selye׳s axioms of adaptation energy together with Goldstone׳s modifications and propose a series of models for interpretation of these axioms. Adaptation energy is considered as an internal coordinate on the 'dominant path' in the model of adaptation. The phenomena of 'oscillating death' and 'oscillating remission' are predicted on the base of the dynamical models of adaptation. Natural selection plays a key role in the evolution of mechanisms of physiological adaptation. We use the fitness optimization approach to study of the distribution of resources for neutralization of harmful factors, during adaptation to a multifactor environment, and analyze the optimal strategies for different systems of factors. Copyright © 2016 Elsevier Ltd. All rights reserved.
Braun, U; Buchli, H; Hässig, M<