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Sample records for coturnix japonica bajo

  1. Spatial orientation in Japanese quails (Coturnix coturnix japonica.

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    Tim Ruploh

    Full Text Available Finding a given location can be based on a variety of strategies, for example on the estimation of spatial relations between landmarks, called spatial orientation. In galliform birds, spatial orientation has been demonstrated convincingly in very young domestic chicks. We wanted to know whether adult Japanese quails (Coturnix coturnix japonica without food deprivation are also able to use spatial orientation. The quails had to learn the relation of a food location with four conspicuous landmarks which were placed in the corners of a square shaped arena. They were trained to find mealworms in three adjacent food cups in a circle of 20 such cups. The rewarded feeders were located during training between the same two landmarks each of which showed a distinct pattern. When the birds had learned the task, all landmarks were displaced clockwise by 90 degrees. When tested in the new situation, all birds redirected their choices with respect to the landmark shift. In subsequent tests, however, the previously correct position was also chosen. According to our results, quails are using conspicuous landmarks as a first choice for orientation. The orientation towards the previously rewarded location, however, indicates that the neuronal representation of space which is used by the birds also includes more fine grain, less conspicuous cues, which are probably also taken into account in uncertain situations. We also presume that the rare orientation towards never rewarded feeders may be due to a foraging strategy instead of being mistakes.

  2. Aspects of the Genotype-Environment Interaction at the Japanese Quail (Coturnix-Coturnix Japonica

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    Teofil Oroian

    2010-05-01

    Full Text Available We analyzed the problems of genotype-environment interaction at three Coturnix Coturnix Japonica varieties. The environment where the experiment took place is perfect identically for all the activities, to ensure that the observed differences at the followed traits to be strictly attributed to the genotype differences. We analyzed the body weight, eggs weight, eggs large and small diameter, yolk and egg white weight, egg-shell weight. The data were statistically interpreted using the average and dispersal indices estimation, and the significance testing using Student test.

  3. The use of scaning electron microscopy in postvaccinal evaluation of tracheal epithelium of Coturnix coturnix japonica

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    Santin Elizabeth

    2003-01-01

    Full Text Available This study aimed at evaluating the use of scanning electron microscopy in the study of the post-vaccinal respiratory reaction of the tracheal epithelium of quails (Coturnix coturnix japonica immunized against Newcastle disease. A number of 36 quails were distributed into four groups: T1 fraction three-quarters control birds (non-vaccinated; T2 fraction three-quarters birds vaccinated with Ulster 2C strain; t3 fraction three-quarters birds vaccinated with B1 strain; t4 fraction three-quarters birds vaccinated with LaSota strain. Regardless the experimental group, birds did not show detectable clinical signs of post-vaccinal respiratory reaction. However, the analysis of tracheal fragments by scanning electron microscopy showed that birds vaccinated with B1 and LaSota strains developed epithelial sloughing of the trachea, whereas those vaccinated Ulster 2C strain did not develop this change, demonstrating intact tracheal epithelium, similar to the control group.

  4. Pemanfaatan Tepung Kulit Buah Terong Belanda (Cyphomandra Betacea) Fermentasi (Aspergillus Niger) Terhadap Pertumbuhan Burung Puyuh (Coturnix-Coturnix Japonica)

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    Nainggolan, Frans H

    2011-01-01

    Frans H Nainggolan, 2008. Utilization of fermented tree tomato (Cyphomandra betacea) skin fruit flour to to performance of Quail (Coturnix-coturnix japonica). Under suvervision of Dr. Ir. Ristika Handarini, MP and Ir. Eniza Saleh, MS. The research caried out in Biological Labolatory, Department of Animal Husbandry, Faculty of Agriculture, North Sumatera University, started from 5 July 2007 until 16 August 2007. The purpose of this experiment was to observe the respon of utilization of ...

  5. Adenocarcinoma renal metastático em uma Codorna Japonesa (Coturnix coturnix japonica Metastatic renal adenocarcinoma in a Japanese Quail (Coturnix coturnix japonica

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    Renata Assis Casagrande

    2011-11-01

    Full Text Available Uma codorna japonesa (Coturnix coturnix japonica, fêmea, adulta com 2 anos apresentou parada de postura, emagrecimento, aumento de volume abdominal e morte. Ao exame macroscópico, observou-se ascite, pulmão esquerdo difusamente esbranquiçado, massas esbranquiçadas, de aproximadamente 1,0cm de diâmetro, na região caudal do lobo direito do fígado e na serosa da moela; massas de 0,1 a 0,5cm no mesentério e serosa intestinal; e uma massa de 5,0x2,0cm na fossa do sinsacro, que acometia 90% do parênquima renal e estendia-se ao ovário e oviduto. Na avaliação histopatológica da neoformação renal, observou-se proliferação de células epiteliais formando túbulos, que muitas vezes não apresentavam lúmen, sustentados por um estroma fibrovascular escasso associado à necrose multifocal. As células neoplásicas eram cuboidais, núcleos redondos a ovalados, nucléolos evidentes, citoplasma abundante e eosinofílico, pleomorfismo moderado e poucas figuras mitóticas. Metástases foram observadas de forma difusa no ovário, oviduto e pulmão esquerdo; multifocal na serosa do intestino e focal no fígado e camadas muscular e serosa da moela. Na avaliação imuno-histoquímica, as células tumorais foram positivas para a citoqueratina (clones AE1+AE3 e negativas para a citoqueratina 5/6, o CD10 e a fosfatase alcalina placentária. De acordo com a localização, achados morfológicos e o perfil imuno-histoquímico, conclui-se que o presente relato trata-se de um adenocarcinoma renal metastático.A 2-year-old Japanese quail (Coturnix coturnix japonica, female, which stopped egg production presented: weight loss, increased abdominal volume, and death. Macroscopically, it was observed: ascites, whitish left lung, whitish masses measuring 1.0cm in diameter in the caudal right lobe of the liver and in the gizzard serosa; 0.1-0.5cm masses in the mesentery and intestinal serosa; and a 5.0x2.0cm mass in synsacral area that encompassed 90% of the renal

  6. Profile Triglycerides Japanese Quail (Coturnix coturnix japonica After Giving Turmeric (Curcuma longa Powder

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    Sukarman Hadi jaya Putra

    2014-12-01

    Full Text Available The purpose of this study was to determine the triglyceride profile of Japanese quail (Coturnix coturnix japonica after being given the parameters of turmeric (Curcuma longa powder; feed intake, profiles of liver triglycerides, profiles of serum triglycerides and profiles of meat triglycerides. This study uses an experimental method with A Completely Randomized Design Pattern. Test animals used were 45 female Japanese quails were divided into 3 groups, namely; P0: quail were not given turmeric powder, P1: quail were given turmeric powder a dose of 54 mg/quail/day, P3: quail were given turmeric powder a dose of 108 mg/quail/day. Each group with 5 replications. Each repeat consists of 3 Japanese quails. Provision of treatment every day for 30 days starting from the age of 14 days. Data were analyzed using analysis of variance (ANOVA followed by Duncan's test with 95% confidence interval (α = 0.05 level. Analysis of the data used is software Minitab software 16. Results showed that the levels of turmeric powder are given in Japanese quail significant (P˂0.05 on liver triglyceride levels, serum and Japanese quail meat but had no significant effect (P˃0.05 on consumption feed. The results showed that the optimal dose of turmeric powder to lower triglyceride levels of Japanese quail is 108 mg/quail/day as evidenced by the highest decrease in liver triglyceride profiles, profiles of serum triglycerides and triglyceride profiles of Japanese quail meat compared with other treatments.

  7. Effect of gamma radiation on mutability in male gametes of Japanese quail (Coturnix coturnix japonica)

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    Baumgartner, J [Vyskumny Ustav Chovu a Slachtenia Hydiny, Laboratorim Genetiky, Ivanka pri Dunaji (Czechoslovakia)

    1978-01-01

    An analysis of the mutability of developmental stages of male gametes of the japanese quail (Coturnix coturnix japonica), after irradiation with /sup 60/Co exposures (5.16; 15.48; and 30.96)x10/sup -2/C kg/sup -1/, i.e. 200, 600 and 1200 R, at an exposure rate of 8.59x10/sup -5/C kg/sup -1/s/sup -1/, i.e. O.33 R s/sup -1/, has shown that the rate of overall induced dominant lethality as well as the rate of mutation were highest in spermatocytes of the second order at all exposures. Starting from an exposure of 200 R the following values were found for lethal interference per 1 gamete for 1 R: spermatozoa 2.0x10/sup -3/, spermatides 1.95x10/sup -3/, spermatocytes II 6.1x10/sup -3/, spermatocytes I 4.06x10/sup -3/, spermatogonia in the process of differentiation 6.8x10/sup -3/. When analysing overall embryonic lethality, according to early and late lethality, dominant lethal mutations mainly manifested themsel--ves in the oviductal and germinal developmental periods, so that these periods can be considered as sensitive indices of genetic changes. The spermatogenic epithelium of the japanese quail was found to be greatly radioresistant since not even an exposure to 1200 R caused complete and permanent sterility.

  8. Impact of vinclozolin on reproductive behavior and endocrinology in Japanese quail (Coturnix coturnix japonica)

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    McGary, S.; Henry, P.F.P.; Ottinger, M.A.

    2001-01-01

    The impact of endocrine-disrupting chemicals (EDCs) has been demonstrated in mammalian models, but less research is available for avian species. The effects of vinclozolin (VIN), an antiandrogenic fungicide, on sexual differentiation and maturation were investigated in Japanese quail (Coturnix coturnix japonica). On day 4 of incubation, embryos were exposed to no treatment, oil, or 25, 50, or 100 ppm of VIN. Endpoints measured included adult male reproductive behavior, hypothalamic gonadotropin-releasing hormone I (GnRH-I) content in hatchlings and adults, plasma steroid levels in hatchlings and adults, proctodeal gland growth during maturation, and relative testicular weight at seven weeks of age. Results showed that exposure to VIN significantly (p < 0.05) altered GnRH-I in male hatchlings, whereas GnRH-I levels in females remained unaffected. Although steroid levels were unaltered by any VIN treatment, the display of male reproductive behavior seemed delayed, with the number of mounts and the number of cloacal contacts being significantly (p < 0.05) lower in the VIN-treated males. This could have an extreme negative impact on wild avian species that are routinely exposed to similar EDCs.

  9. Ovarian radiographic and direct measurements of Japanese Quail ( Coturnix coturnix japonica submitted to light restriction

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    M. Albuquerque

    Full Text Available ABSTRACT Often, in pet birds, any stimulus to lay eggs is unwanted in order to reduce reproductive diseases and disorders. The objectives of this study were: to determine the time necessary to promote ovary involution after an eight hour photoperiod using laying Japanese quails (Coturnix coturnix japonica; to connect the ovarian radiographic measurements with egg production; and to compare these measurements with direct ovary data obtained at necropsy. Birds were separated into three groups: 12h/24d (control group - 12h photoperiod for 24 days, 8h/24d and 8h/36d (8h photoperiod for 24 and 36 days. After euthanasia, all cadavers were x-rayed to measure ovary length and height. Birds were necropsied to measure ovarian length and weight. Results: radiographic ovary length demonstrated strong and positive correlation (r=0.96 with direct ovary length of all three groups; laying quails showed higher ovary height (p=0.025 and length (p=0.009 than non-laying quails; eight hours of artificial light per day promotes ovary length (p=0.025 and weight (p=0.009 reduction. Conclusions: radiography can estimate the ovary measure and indicate posture; an eight hour photoperiod of 24 days is not enough to promote ovarian regression, while the use of reduced photoperiod for 36 days promotes significant ovary involution.

  10. Efeito da ingestão de fumonisina B1 no peso corporal e na histopatologia de codornas japonesas (Coturnix coturnix japonica Effect of fumonisin B1 on body weight and histopathology of Japanese quail (Coturnix coturnix japonica

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    Carlos Augusto Fernandes Oliveira

    2007-02-01

    Full Text Available O objetivo do presente trabalho foi estudar o efeito da fumonisina B1 (FB1 sobre o peso corpóreo e as vísceras (fígado, rim e coração de codorna poedeira japonesa (Coturnix coturnix japonica, devido ao escasso dado toxicológico nesta espécie. Quatro grupos, sendo cada um constituído de 32 codornas de linhagem comercial, receberam ração contendo FB1 nas concentrações de 0 (controle, 10, 50 e 250mg kg-1, durante 28 dias. Observou-se uma redução (P0,05, porém as aves do grupo 50 e 250mg kg-1 apresentaram aumento no peso relativo de fígado (PThis research was aimed at evaluating the effect of fumonisin B1 (FB1 on body weight and viscera (liver, kidney and heart of laying Japanese quail (Coturnix coturnix japonica, due to the little toxicological data on this species. Four experimental groups of 32 commercial quails were designed and exposed to ingestion test with feed containing 0 (controls, 10, 50 or 250mg FB1 kg-1 feed, during 28 days. Birds of group 250mg FB1 kg-1 showed lower body weight (P0.05 among treatments. However, the relative weight of liver increased (P<0.05 in quail of groups received 50 and 250mg FB1/kg. Histological changes were observed only in the liver of groups receiving 50 and 250mgFB1 kg-1, which caused moderate biliary duct hyperplasia and multiple foci of hepatic necrosis. The data indicated that AFB1 at levels above 50mg kg-1 in laying Japanese quail.

  11. A survey of Japanese quail (Coturnix coturnix japonica farming in selected areas of Bangladesh

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    Abu Nasar Md. Aminoor Rahman

    2016-09-01

    Full Text Available Aim: To investigate the status, problems and prospects of Japanese quail (Coturnix coturnix japonica farming in selected areas of Bangladesh. Materials and Methods: The study was conducted in 14 districts of Bangladesh, viz., Dhaka, Narayanganj, Munshiganj, Mymensingh, Netrakona, Faridpur, Jessore, Khulna, Satkhira, Kushtia, Bogra, Naogaon, Comilla, and Sylhet during the period from July 2011 to June 2012. A total of 52 quail farmers were interviewed for data collection using a structured questionnaire. Focus group discussions were also carried out with unsuccessful farmers and those want to start quail farming. Workers of quail farms, quail feeds and medicine suppliers, quail eggs and meat sellers were also interviewed regarding the issue. Results: Out of 52 farms, 86.5% were operated by male, 67.3% farmers did not receive any training and 92.3% farmers had no earlier experience of quail farming although 58.0% farmers primary occupation was quail farming. Most of the farms (63.4% were mixed in type having ≤5000 birds of two or three varieties. About 80.7% farms were operated separately round the year with no other poultry and 83.0% farmers wanted to expand their farming. The average pullet weight 14.5±0.12, 11.0±0.07, 12.0±0.22, and 12.8±0.17 g; age at the first lay 46.0±0.04, 42.0±0.31, 42.0±0.09, and 45.2±0.05 days; rearing period 15.0±0.01, 12.0±0.14, 15.0±0.32, and 15.2±0.18 months; culling period 15.5±0.14, 13.0±0.06, 15.0±0.03, and 15.4±0.26 months were for layer, parent stock, hatchery, and mixed farms, respectively. Most of the layer farms had an average egg production of ≤5000/day and net profit BDT 0.75/egg. However, an average number of birds, hatchability and net profit per day-old-chick were ≤5000, 76.8% and BDT 2.75, respectively, in the hatchery. Broiler quails were sold at 30 days with mean weight of 110.8 g and net profit BDT 9.02/bird. The major constraints of quail farming were higher feed price

  12. A survey of Japanese quail (Coturnix coturnix japonica) farming in selected areas of Bangladesh.

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    Nasar, Abu; Rahman, Aminoor; Hoque, Nazmul; Kumar Talukder, Anup; Das, Ziban Chandra

    2016-09-01

    To investigate the status, problems and prospects of Japanese quail (Coturnix coturnix japonica) farming in selected areas of Bangladesh. The study was conducted in 14 districts of Bangladesh, viz., Dhaka, Narayanganj, Munshiganj, Mymensingh, Netrakona, Faridpur, Jessore, Khulna, Satkhira, Kushtia, Bogra, Naogaon, Comilla, and Sylhet during the period from July 2011 to June 2012. A total of 52 quail farmers were interviewed for data collection using a structured questionnaire. Focus group discussions were also carried out with unsuccessful farmers and those want to start quail farming. Workers of quail farms, quail feeds and medicine suppliers, quail eggs and meat sellers were also interviewed regarding the issue. Out of 52 farms, 86.5% were operated by male, 67.3% farmers did not receive any training and 92.3% farmers had no earlier experience of quail farming although 58.0% farmers primary occupation was quail farming. Most of the farms (63.4%) were mixed in type having ≤5000 birds of two or three varieties. About 80.7% farms were operated separately round the year with no other poultry and 83.0% farmers wanted to expand their farming. The average pullet weight 145.0±0.12, 110.0±0.07, 120.0±0.22, and 128.0±0.17 g; age at the first lay 46.0±0.04, 42.0±0.31, 42.0±0.09, and 45.2±0.05 days; rearing period 15.0±0.01, 12.0±0.14, 15.0±0.32, and 15.2±0.18 months; culling period 15.5±0.14, 13.0±0.06, 15.0±0.03, and 15.4±0.26 months were for layer, parent stock, hatchery, and mixed farms, respectively. Most of the layer farms had an average egg production of ≤5000/day and net profit BDT 0.75/egg. However, an average number of birds, hatchability and net profit per day-old-chick were ≤5000, 76.8% and BDT 2.75, respectively, in the hatchery. Broiler quails were sold at 30 days with mean weight of 110.8 g and net profit BDT 9.02/bird. The major constraints of quail farming were higher feed price, outbreak of endemic diseases, lack of proper knowledge

  13. Effects of Propolis, Royal Jelly, Bee Pollen and Ronozyme Supplementation in Diets of Japanese Quails (Coturnix Coturnix Japonica) on Yolk Lipid Peroxidation

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    Yılmaz S; Tatli Seven P; Kaya E

    2017-01-01

    This study discovers the possible effect propolis, royal jelly, bee pollen and Ronozyme supplementation in diets that can be beneficial for Japanese quails (Coturnix coturnix japonica). Total one hundred and sixty Japanese quails at 43 days of age were used and divided randomly into 4 replicate groups each containing 32 animals. The experimental groups as follows: control group was feed a basal diet, royal jelly group was added to the water with 500 mg/kg diet, propolis group was feed orally ...

  14. Comparative pharmacokinetics of enrofloxacin, danofloxacin, and marbofloxacin after intravenous and oral administration in Japanese quail (Coturnix coturnix japonica).

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    Haritova, Aneliya; Dimitrova, Dimitrichka; Dinev, Toncho; Moutafchieva, Rumyana; Lashev, Lubomir

    2013-03-01

    A population approach was used to evaluate the pharmacokinetic parameters of 3 fluoroquinolones administered to Japanese quail (Coturnix coturnix japonica). Healthy adult quail (n = 50) were divided into 3 groups, each administered a separate intravenous and oral dose of the compounded drug: enrofloxacin at 10 mg/kg (n = 18; 9 male, 9 female), danofloxacin at 10 mg/kg (n = 12; 6 male, 6 female), and marbofloxacin at 5 mg/kg (n = 20; 10 male, 10 female). A fourth group was used as a control (n = 5). Enrofloxacin was metabolized extensively to ciprofloxacin, while no metabolites of either danofloxacin or marbofloxacin were detected. The volume of distribution was high, greater than 1 in all cases, and highest for danofloxacin, followed by enrofloxacin, then marbofloxacin. The total body clearance was higher in quail than that reported for other avian species with the exception of ostriches. As in mammals, the lowest clearance rate of the 3 fluoroquinolones was observed for marbofloxacin. Enrofloxacin was absorbed most rapidly, followed by marbofloxacin, then danofloxacin. The highest bioavailability was observed for danofloxacin followed by marbofloxacin, while very low bioavailability with significant conversion to ciprofloxacin was observed for enrofloxacin. Population analysis showed low intersubject variability for danofloxacin and marbofloxacin in contrast to that for enrofloxacin and its main metabolite, ciprofloxacin. Because of their more favorable pharmacokinetic properties after oral administration, either danofloxacin or marbofloxacin appears to be preferable to enrofloxacin for the treatment of susceptible bacterial infection in Japanese quail.

  15. Effect of feed supplement containing earthworm meal (Lumbricus rubellus) on production performance of quail (Coturnix coturnix japonica)

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    Istiqomah, L.; Sakti, A. A.; Suryani, A. E.; Karimy, M. F.; Anggraeni, A. S.; Herdian, H.

    2017-12-01

    The objective of this study was to evaluate the effect of feed supplement (FS) contained earthworm meal (EWM) on production performance of laying quails. Twenty weeks-old of 360 Coturnix coturnix japonica quails were used in a Completely Randomized Design (CRD) with three dietary treatments A = CD (control without FS), B = CD + 0.250 % of FS, and C = CD + 0.375 % of FS during 6 weeks of experimental period. Each treatment in 4 equal replicates in which 30 quails were randomly allocated into 12 units of cages. Variable measured were feed intake, feed conversion ratio, feed efficiency, mortality rate, hen day production, egg weight, and egg uniformity. Data were statistically analyzed by One Way ANOVA and the differences among mean treatments are analysed using Duncan’s Multiple Range Test (DMRT). The results showed that administration of 0.375% FS based on earthworm meal, fermented rice bran, and skim milk impaired the feed conversion ratio and increased the feed efficiency. The experimental treatments did not effect on feed intake, mortality, hen day production, egg weight, and egg uniformity of quail. It is concluded that administration of feed supplement improved the growth performance of quail.

  16. Eimeria spp. from Japanese quails (Coturnix japonica: new characteristic features and diagnostic tools

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    Bruno P. Berto

    2013-12-01

    Full Text Available The Japanese quail Coturnix japonica originated from North Africa, Europe and Asia, is used worldwide as an experimental animal and model for aviculture. The current paper characterizes Eimeria bateri, Eimeria tsunodai and Eimeria uzura recovered from C. japonica. Based on the fact that quails have a global distribution, as are their coccidia, the findings of this study should provide the means for diagnosis of those Eimeria spp. in other regions and continents. Eimeria bateri showed the greatest intensity of infection and shed oocysts from the fourth day after infection; in contrast, E. tsunodai and E. uzura shed oocysts from the fifth day after infection. The three species shared a high degree of similarity and were all polymorphic. Yet, the application of line regressions, histograms and ANOVA provided means for the identification of these species. Finally, the algorithm was very efficient since verified that resultant values were not superimposed.

  17. Effect of photoperiod on body weight gain, and daily energy intake and energy expenditure in Japanese quail (Coturnix c. Japonica)

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    Boon, P; Visser, GH; Daan, S

    2000-01-01

    Effect of photoperiod and food duration on body weight gain, energy intake, energy expenditure, and sexual development were investigated in two strains of Japanese quail (Coturnix c. japonica), bred for meat (broilers) or egg production (layers), from 7 to 71 days of age. In a first experiment

  18. Effects of selection for yolk cholesterol on growth and meat quality in Japanese quail (Coturnix coturnix japonica

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    Marek Bednarczyk

    2010-01-01

    Full Text Available Descendants ( of S18 generation of Japanese quails (Coturnix coturnix japonica, selected for low (line 11, n=15 or for high (line 12, n=6 yolk cholesterol content were used for this experiment. The high (12 and low (11 lines were developed from a control egg type (line13 wild color plumage stock 07, from Poultry Breeding Station in Ivanka at Danube (Slovakia that was maintained without conscious selection. The results of selected lines were compared to this one from unselected lines, egg type (line 13, n=15 and meat type (line 8, n=15, to study productive performance (growth and carcass weight as well as meat quality traits (intramuscular collagen properties, fatty and cholesterol content. The birds were grown up to 35 days of age. Quails were fed ad libitum with commercial diets according to their ages. Animals had free access to water during the experiment. The body weight of the quails was controlled every week (from week 1 to 5. At the slaughter (at avg. weight of 104.0, 107.7, 110.6 g vs 209.3 g for lines 11, 12, 13 vs 8, respectively; P<0.05 the hot carcass weight was recorded and dressing percentage was calculated. Pectoralis superficialis muscles were removed from the carcasses to assess intramuscular collagen (IMC properties (collagen and crosslink concentrations, intramuscular lipid and cholesterol contents. Growth and slaughter performance, intramuscular fat percentage and IMC properties of Pectoralis superficialis were not different between the birds selected for low (line 11 and high (line 12 yolk cholesterol content and were also found to be similar between the quails of egg type and those selected for yolk cholesterol content (lines 11 and 12. Compared to the birds of egg type line and those selected for yolk cholesterol content, the quails of meat type had higher growth and carcass traits (P<0.05, while meat quality traits were found to be similar. The meat of the quails selected for low cholesterol in the egg yolk (line 11

  19. Study on the effect of dry extract of Tribulus terrestris on the forage consumption rate in japanese quail (Coturnix coturnix japonica

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    Dimo PENKOV

    2016-02-01

    Full Text Available An investigation of Vemo Herb® additive on the forage consumption rate in Japanese quail (Pharaoh breed has been conducted. The experiment was carried out for 10 weeks with 52 female and 16 male sexually matured Japanese quails (Coturnix coturnix japonica distributed in four groups: control group (without additive, first experimental group - 4 mg*kg-1 of live weight for 10 weeks; second experimental group - 10 mg*kg-1 of live weight during the first five weeks of the study (after that the product was no more supplemented and third experimental group - 10 mg*kg-1 of live weight during the whole study period. It was observed, a tendency of decreasing of the forage consumption rate for the whole experimental period (no statistical significance for all the experimental groups compared to the control group, but some of the weekly differences are significant.

  20. Effect of dietary supplementation of fermented fish silage on serum biochemical parameters of broiler Japanese quails (Coturnix coturnix japonica

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    Laxman Kumar Babu

    2017-04-01

    Full Text Available Aim: The objective of this study was to evaluate the effect of feeding fermented fish silage (FFS on serum biochemical parameters of Japanese quails (Coturnix coturnix japonica. Materials and Methods: A total of 192, 7-day-old broiler Japanese quail chicks of either sex, were randomly distributed into four dietary treatments with four replicates in each group having 12 chicks in each replicate pen. The dietary treatments were T1 - Control diet, T2 - Diet containing 5% FFS, T3 - Diet containing 10% FFS, and T4 - Diet containing 15% FFS. Group body weight and feed consumption were recorded at weekly intervals. Feed conversion ratio (FCR was derived by dividing the feed consumed with the weekly body weight gain. At the end of the experiment, 8 birds from each treatment were selected randomly and sacrificed by cervical dislocation to study the carcass traits expressed as % pre-slaughter live weight. At 5 weeks of age, about 2 ml of blood was taken from the jugular vein of each selected bird, and serum samples were separated after centrifugation. Total protein, albumin/globulin (A/G ratio, calcium, phosphorus, triglyceride, total cholesterol, high density lipoprotein-cholesterol (HDL-C, very low density lipoprotein-cholesterol (VLDL-C, low density lipoprotein-cholesterol (LDL-C, alanine aminotransferase (ALT, and aspartate aminotransferase (AST were estimated in the serum. Results: The cumulative body weight gains from 1 to 5 weeks of age did not vary significantly between control and 5% FFS group. However, a linear decrease in body weight gain was observed by increasing the levels of FFS beyond 5% in the diet. The cumulative feed consumption was significantly higher in case of birds under control group during 1-5 weeks of age as compared to birds fed FFS based diet (5%, 10%, and 15%. No statistical difference in cumulative feed conversion ratio could be noticed during 1-5 weeks of age. The eviscerated yield decreased and giblet weight increased due

  1. Spontaneous, Experimentally Induced, and Transmissible AA Amyloidosis in Japanese Quail ( Coturnix japonica).

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    Nakayama, Yumi; Kamiie, Junichi; Watanabe, Gen; Suzuki, Kazuhiko; Murakami, Tomoaki

    2017-11-01

    The authors describe a spontaneous case of amyloid A (AA) amyloidosis in an adult female Japanese quail ( Coturnix japonica). The bird developed AA amyloidosis secondary to chronic peritonitis caused by a Gram-negative bacillus infection. Mild amyloid deposition was also identified in the intestinal tract of apparently healthy adult individuals, suggesting that quail may develop intestinal amyloidosis with age. Based on these observations, it was hypothesized that quail can develop AA amyloidosis following inflammatory stimulation with lipopolysaccharide (LPS). Therefore, adult quail were repeatedly injected with LPS and the development of AA amyloidosis was confirmed. The amyloid deposition in this model increased when quail amyloid was intravenously injected as an amyloid-enhancing factor. The experiments were repeated with young quail, but amyloid deposits were not observed following LPS injections. However, AA amyloidosis did develop when quail amyloid was injected in addition to LPS. These results indicated that adult quail develop AA amyloidosis after inflammatory stimulation with LPS. Furthermore, quail AA amyloidosis was shown to have transmissibility regardless of age. Interestingly, the authors found that administration of chicken amyloid fibrils also induced AA amyloidosis in young quail. This is the first report of cross-species transmission of avian AA amyloidosis.

  2. Evaluation of pomegranate (Punica granatum pericarp aqueous extract on Eimeria spp. from Japanese quails (Coturnix japonica

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    Bruno P. Berto

    2014-06-01

    Full Text Available Antibacterial, anti-inflammatory and antiparasitic properties have been associated with the extract of pomegranate (Punica granatum in several animals and conditions. The Japanese quail (Coturnix japonica, originated from North Africa, Europe and Asia, is used worldwide as an experimental animal and model for aviculture. The current study investigated the effects of the pomegranate pericarp aqueous extract on the shedding, viability and morphometry of three Eimeria spp. from Japanese quails, besides the weight gain and genotoxic activity. Although the pomegranate is recognized by multiple properties, including anti-coccidial, in the current study the results are contrary. The treated group shed greater amount of oocysts; the sporulation times and viability were similar in both groups; despite some morphometric differences, these were not expressive; weight gains were similar; and the pomegranate had insignificant effect genotoxic. Finally, these results suggest that the pomegranate pericarp extract did not influence on Eimeira spp. from Japanese quails; therefore, the pomegranate pericarp extract is not suggested in the prevention/treatment of coccidiosis in Japanese quails, or at least not using methods of preparation and administration applied in this study.

  3. Effect of different levels of alpha tocopherol on performance traits, serum antioxidant enzymes, and trace elements in Japanese quail ( Coturnix coturnix japonica under low ambient temperature

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    Assar Ali Shah

    Full Text Available ABSTRACT This study was designed to find the effect of vitamin E supplementation on growth, serum antioxidant enzymes, and some trace elements in Japanese quail (Coturnix coturnix japonica under low ambient temperature. A total of 180 day-old Japanese quails were randomly divided into four groups and provided with 0 (group A, 50 (group B, 100 (group C, and 150 IU/kg (group D vitamin E (dl-α-tocopherol acetate under an average temperature of 9±0.5 °C for an experimental period of 42 days. The result showed that feed intake per day, body weight, weight gain per day, and feed conversion ratio did not differ significantly between the groups. Serum concentrations of superoxide and glutathione peroxidase were significantly high in birds supplemented with 150 mg/kg of vitamin E. The concentration of aspartate aminotransferase was not significantly affected between the control and treated groups; however, alanine transaminase concentration significantly reduced in group D. Zinc concentration in the blood increased significantly in group D, with no significant effect on copper and manganese between the control and treated groups. Vitamin E at the level of 150 IU/kg of feed improves the blood antioxidant status and zinc concentration, with no effect on the performance traits of quail reared under low ambient temperature.

  4. Effect of dietary supplementation of hemp seed (Cannabis sativa L.) on meat quality and egg fatty acid composition of Japanese quail (Coturnix coturnix japonica).

    Science.gov (United States)

    Yalcin, H; Konca, Y; Durmuscelebi, F

    2018-02-01

    This study was conducted in two separate experiments. Experiment I was conducted to investigate the effects of hemp seed (HS) on meat quality traits, and experiment II was designed to determine egg fatty acid (FA) composition in Japanese quail (Coturnix coturnix japonica). In experiment I and experiment II treatments, groups were as follows: (i) control (not included HS0), (ii) 5% HS inclusion to diets, (iii) 10% HS inclusion to diets and (iv) 20% HS inclusion to diets. In experiment I, a total of 192 7-day-old-quail were fed with HS diets for 5 weeks, and end of the experiment I, a total of 64 quail (16 each) slaughtered and meat samples were analysed for meat colour, pH, cooking and thawing loss and FA composition. In experiment II, a total of 120 8-week-old laying quail were fed with experimental diets for 6 weeks and egg FA composition were determined. Breast meat cooking loss was significantly lower in 20% supplemented group (p egg samples were linearly increased with increasing the dietary HS ratio (p eggs (p egg yolk omega-3 FA content and decrease cooking loss of quail meats. © 2017 Blackwell Verlag GmbH.

  5. Body weight, egg production, and egg quality traits of gray, brown, and white varieties of Japanese quail (Coturnix coturnix japonica in coastal climatic condition of Odisha

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    Jessy Bagh

    2016-08-01

    Full Text Available Aim: The present study was conducted to evaluate the performance of gray, brown, and white varieties of Japanese quail (Coturnix coturnix japonica with respect to body weight, egg production, and egg quality traits in the coastal climatic condition of Odisha. Materials and Methods: A total of 500-day-old straight run Japanese quail chicks of three varieties, viz., gray, brown, and white were randomly selected and reared in deep litter system at Central Poultry Development Organization, Eastern Region, Bhubaneswar. The weekly body weight of the birds was recorded till their egg production stage (up to 6 weeks of age. The average egg production was recorded every biweekly from 6th to 20th week. Exterior and interior quality of eggs from each variety was determined at 6 weeks of age. Results: The initial average weekly body weight of three varieties did not differ (p>0.05 among the varieties. However, from 1st to 6th week significantly higher body weight was observed in gray than white and brown. Brown varieties had reached 50% egg production 1 week earlier than gray and white. Brown had higher peak hen day (HD production or henhoused egg production followed by white and gray. External quality such as: Egg weight, egg length, egg width, volume, shape index, shell weight, shell thickness depicted no significant difference among the varieties except circumference length and circumference width, which were significantly higher (p≤0.05 in gray varieties than brown varieties. Internal egg characteristics such as: Albumen length, albumen width, albumen height, albumen index, yolk length, yolk width, yolk height, yolk index, albumen weight, yolk weight, Haugh unit revealed no significance difference among the varieties. Conclusion: It may be summarized from the findings that gray excelled in body weight followed by white and brown. Egg production potential in terms of hen house egg production or HD egg production was higher for brown followed by white

  6. Comprehensive Identification of Sexual Dimorphism-Associated Differentially Expressed Genes in Two-Way Factorial Designed RNA-Seq Data on Japanese Quail (Coturnix coturnix japonica.

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    Kelsey Caetano-Anolles

    Full Text Available Japanese quail (Coturnix coturnix japonica reach sexual maturity earlier, breed rapidly and successfully, and cost less and require less space than other birds raised commercially. Given the value of this species for food production and experimental use, more studies are necessary to determine chromosomal regions and genes associated with gender and breed-differentiation. This study employed Trinity and edgeR for transcriptome analysis of next-generation RNA-seq data, which included 4 tissues obtained from 3 different breeding lines of Japanese quail (random bred control, heavy weight, low weight. Differentially expressed genes shared between female and male tissue contrast groups were analyzed to identify genes related to sexual dimorphism as well as potential novel candidate genes for molecular sexing. Several of the genes identified in the present study as significant sex-related genes have been previously found in avian gene expression analyses (NIPBL, UBAP2, and other genes found differentially expressed in this study and not previously associated with sex-related differences may be considered potential candidates for molecular sexing (TERA, MYP0, PPR17, CASQ2. Additionally, other genes likely associated with neuronal and brain development (CHKA, NYAP, as well as body development and size differentiation (ANKRD26, GRP87 in quail were identified. Expression of homeobox protein regulating genes (HXC4, ISL1 shared between our two sex-related contrast groups (Female Brain vs. Male Brain and Ovary vs. Testis indicates that these genes may regulate sex-specific anatomical development. Results reveal genetic features of the quail breed and could allow for more effective molecular sexing as well as selective breeding for traits important in commercial production.

  7. Plasma Concentrations of Itraconazole, Voriconazole, and Terbinafine When Delivered by an Impregnated, Subcutaneous Implant in Japanese Quail ( Coturnix japonica ).

    Science.gov (United States)

    Souza, Marcy J; Redig, Patrick; Cox, Sherry K

    2017-06-01

    Aspergillosis is a common fungal infection in both wild and pet birds. Although effective antifungal medications are available, treatment of aspergillosis can require months of medication administration, which entails stressful handling one or more times per day. This study examined the delivery of the antifungal drugs itraconazole, voriconazole, and terbinafine to Japanese quail ( Coturnix japonica ) via an impregnated implant. Implants contained 0.5, 3, 8, or 24 mg of itraconazole, voriconazole, or terbinafine. The implants were administered subcutaneously over the dorsum and between the scapulae. Blood was collected from birds before and 2, 7, 21, 42, and 56 days after implant placement. Plasma was analyzed by high-performance liquid chromatography for concentrations of itraconazole, voriconazole, or terbinafine, as appropriate. During the course of the study, targeted terbinafine concentrations were achieved in some birds at various time points, but concentrations were inconsistent. Itraconazole and voriconazole concentrations were also inconsistent and did not reach targeted concentrations. Currently, the implant examined in this study cannot be recommended for treatment of aspergillosis in avian species.

  8. No evidence for sex-specific effects of the maternal social environment on offspring development in Japanese quail (Coturnix japonica).

    Science.gov (United States)

    Langen, Esther M A; von Engelhardt, Nikolaus; Goerlich-Jansson, Vivian C

    2018-07-01

    The social environment of reproducing females can cause physiological changes, with consequences for reproductive investment and offspring development. These prenatal maternal effects are often found to be sex-specific and may have evolved as adaptations, maximizing fitness of male and female offspring for their future environment. Female hormone levels during reproduction are considered a potential mechanism regulating sex allocation in vertebrates: high maternal androgens have repeatedly been linked to increased investment in sons, whereas high glucocorticoid levels are usually related to increased investment in daughters. However, results are not consistent across studies and therefore still inconclusive. In Japanese quail (Coturnix japonica), we previously found that pair-housed females had higher plasma androgen levels and tended to have higher plasma corticosterone levels than group-housed females. In the current study we investigate whether these differences in maternal social environment and physiology affect offspring sex allocation and physiology. Counter to our expectations, we find no effects of the maternal social environment on offspring sex ratio, sex-specific mortality, growth, circulating androgen or corticosterone levels. Also, maternal corticosterone or androgen levels do not correlate with offspring sex ratio or mortality. The social environment during reproduction therefore does not necessarily modify sex allocation and offspring physiology, even if it causes differences in maternal physiology. We propose that maternal effects of the social environment strongly depend upon the type of social stimuli and the timing of changes in the social environment and hormones with respect to the reproductive cycle and meiosis. Copyright © 2018 Elsevier Inc. All rights reserved.

  9. COMPARISON OF TWO 4.7-MILLIGRAM TO ONE 9.4-MILLIGRAM DESLORELIN ACETATE IMPLANTS ON EGG PRODUCTION AND PLASMA PROGESTERONE CONCENTRATIONS IN JAPANESE QUAIL (COTURNIX COTURNIX JAPONICA).

    Science.gov (United States)

    Petritz, Olivia A; Guzman, David Sanchez-Migallon; Hawkins, Michelle G; Kass, Philip H; Conley, Alan J; Paul-Murphy, Joanne

    2015-12-01

    Reproductive disease in captive avian species is common, and medical management is often chosen over surgical removal of the reproductive tract. In a previous study with Japanese quail, a single 4.7-mg deslorelin acetate implant reversibly decreased egg production in 6 out 10 birds for 70 days. The objective of the current study was to evaluate the effects of two 4.7-mg deslorelin acetate implants versus one 9.4-mg implant on egg production and plasma progesterone concentrations in Japanese quail ( Coturnix coturnix japonica). Following a 10-day period of consistent egg laying, 30 adult female Japanese quail were anesthetized and received two 4.7-mg deslorelin implants (n = 10), one 9.4-mg deslorelin implant (n = 10), or a single, identical placebo implant (n = 10) s.c. between the scapulae. Egg production was monitored daily, and plasma progesterone concentrations were measured on days 0, 14, 29, 120, 148, and 182 via enzyme-linked immunoassay. All birds were weighed periodically and euthanized at day 182, after which their reproductive tracts were evaluated at gross necropsy. Seven out of 10 birds treated with two 4.7-mg implants ceased egg laying 1 wk after implantation and remained nonovulatory for approximately 100 days. Cessation of egg laying for the 9.4-mg treatment group occurred in 7 out of 10 birds; onset was variable (weeks 5-12) and continued for the remainder of the study period. Plasma progesterone concentrations for deslorelin treatment groups were not significantly different compared to the placebo group at any time point. In conclusion, the two 4.7-mg and the one 9.4-mg implant treatments ceased egg laying in a similar number of birds, but the 9.4-mg implant had a slower onset of action and the effects on egg laying were inconsistent throughout the study period. Further studies evaluating use of deslorelin acetate in other avian species are needed.

  10. Índice gonadossomático e correlações entre dimensões e peso testiculares na codorna japonesa (Coturnix coturnix japonica aos 60 dias de idade

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    L.L. Lanna

    2013-08-01

    Full Text Available As dimensões e o peso testicular foram avaliados em 25 machos adultos de codornas japonesas (Coturnix coturnix japonica e correlacionados entre si, a fim de se buscar obter informações que possibilitem a criação de um parâmetro preditivo da capacidade de produção espermática. As aves foram abatidas e os testículos removidos para mensuração e pesagem. As dimensões e o peso entre os testículos esquerdo e direito foram comparados. Os parâmetros testiculares foram correlacionados entre si por meio da determinação do coeficiente de correlação linear. O índice gonadossomático também foi determinado, indicando alocação de 3,68% do peso corporal em testículos nos machos avaliados, resultado maior do que os descritos na literatura. Apesar da diferença de formato entre os testículos esquerdo e direito, o peso foi semelhante, sugerindo que não há diferença na capacidade de produção espermática entre eles. Diferença estatística entre os valores biométricos indica que o testículo esquerdo é mais curto e mais arredondado, enquanto o direito é mais longo e estreito. O peso corporal apresentou baixa correlação com o peso testicular, 0,14 e 0,12 para os testículos esquerdo e direito, respectivamente. Os parâmetros que melhor se correlacionaram com o peso testicular foram a largura e a espessura. Considerando-se a identificação de um parâmetro único para comparação entre machos, a espessura do testículo esquerdo apresentou coeficiente de correlação linear de 0,89 com o somatório do peso dos dois testículos. Com base nessa informação, métodos não invasivos, como a ultrassonografia, poderiam ser utilizados para estimar o potencial reprodutivo e auxiliar na comparação de machos de codornas japonesas em núcleos de seleção de aves elite, contribuindo para melhoramento genético da espécie.

  11. Species specificity of social reinstatement in Japanese quail Coturnix japonica genetically selected for high or low levels of social reinstatement behaviour.

    Science.gov (United States)

    Mills, A D; Jones, R B; Faure, J M

    1995-05-01

    Divergent lines of Japanese quail Coturnix japonica showing high (HSR) or low (LSR) levels of social reinstatement (SR) behaviour (as measured in a treadmill apparatus) have been developed. However, it was not known if selection had influenced social reinstatement tendencies in a general or a species-specific fashion. Therefore, the present study compared the SR behaviour of quail chicks of the HSR and LSR lines and of a Control line when the goal box of the treadmill was empty or when it contained small, same-species groups of either Japanese quail, domestic fowl or Guinea fowl chicks. The results clearly demonstrated that the SR behaviour of Japanese quail chicks is species-specific and that this specificity has not been influenced during genetic selection, over sixteen generations, of the HSR and LSR lines. The HSR chicks showed more locomotor activity in the treadmill than did those of the other lines regardless of the nature of the goal-box stimulus. The results are discussed in terms of general activity, underlying fearfulness and social motivation.

  12. Toxicity of Pb-contaminated soil to Japanese quail (Coturnix japonica) and the use of the blood-dietary Pb slope in risk assessment

    Science.gov (United States)

    Beyer, W. Nelson; Chen, Yu; Henry, Paula; May, Thomas; Mosby, David; Rattner, Barnett A.; Shearn-Bochsler, Valerie I.; Sprague, Daniel; Weber, John

    2014-01-01

    This study relates tissue concentrations and toxic effects of Pb in Japanese quail (Coturnix japonica) to the dietary exposure of soil-borne Pb associated with mining and smelting. From 0% to 12% contaminated soil, by weight, was added to 5 experimental diets (0.12 to 382 mg Pb/kg, dry wt) and fed to the quail for 6 weeks. Benchmark doses associated with a 50% reduction in delta-aminolevulinic acid dehydratase activity were 0.62 mg Pb/kg in the blood, dry wt, and 27 mg Pb/kg in the diet. Benchmark doses associated with a 20% increase in the concentration of erythrocyte protoporphyrin were 2.7 mg Pb/kg in the blood and 152 mg Pb/kg in the diet. The quail showed no other signs of toxicity (histopathological lesions, alterations in plasma–testosterone concentration, and body and organ weights). The relation of the blood Pb concentration to the soil Pb concentration was linear, with a slope of 0.013 mg Pb/kg of blood (dry wt) divided by mg Pb/kg of diet. We suggest that this slope is potentially useful in ecological risk assessments on birds in the same way that the intake slope factor is an important parameter in risk assessments of children exposed to Pb. The slope may also be used in a tissue-residue approach as an additional line of evidence in ecological risk assessment, supplementary to an estimate of hazard based on dietary toxicity reference values.

  13. Social environment during egg laying: Changes in plasma hormones with no consequences for yolk hormones or fecundity in female Japanese quail, Coturnix japonica.

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    Esther M A Langen

    Full Text Available The social environment can have profound effects on an individual's physiology and behaviour and on the transfer of resources to the next generation, with potential consequences for fecundity and reproduction. However, few studies investigate all of these aspects at once. The present study housed female Japanese quail (Coturnix japonica in pairs or groups to examine the effects on hormone concentrations in plasma and yolk and on reproductive performance. Circulating levels of androgens (testosterone and 5-α-dihydrotestosterone and corticosterone were measured in baseline samples and after standardised challenges to assess the responsiveness of the females' endocrine axes. Effects of the social environment on female fecundity were analysed by measuring egg production, egg mass, fertilization rates, and number of hatched offspring. Counter to expectation, females housed in pairs had higher plasma androgen concentrations and slightly higher corticosterone concentrations than females housed in groups, although the latter was not statistically significant. Pair vs. group housing did not affect the females' hormonal response to standardised challenges or yolk testosterone levels. In contrast to previous studies, the females' androgen response to a gonadotropin-releasing hormone challenge was not related to yolk testosterone levels. Non-significant trends emerged for pair-housed females to have higher egg-laying rates and higher fertility, but no differences arose in egg weight or in the number, weight or size of hatchlings. We propose that our unexpected findings are due to differences in the adult sex ratio in our social treatments. In pairs, the male may stimulate female circulating hormone levels more strongly than in groups where effects are diluted due to the presence of several females. Future studies should vary both group size and sex composition to disentangle the significance of sexual, competitive and affiliative social interactions for

  14. EFECTO DEL USO DE ADITIVOS EN DIETAS DE CODORNICES REPRODUCTORES (Coturnix coturnix japonica) BAJO CONDICIONES DE VERANO EN LA COSTA CENTRAL

    OpenAIRE

    Ciriaco Castañeda, Pedro; Facultad Zootecnia, Dpto. de Produccion Animal de la Universidad Nacional Agraria La Molina (Perú).; Roncal Ñañez, Henry David; Ing. Zootecnista (Perú).

    2016-01-01

    Se evaluaron los efectos de la adición en la dieta de 0.05% del Complejo B, 0.05% de Vitamina C, 0.2% de Bicarbonato de Sodio y 0.05% de Ácido Acetil Salicílico, sobre los parámetros productivos y reproductivos de codornices japonesas en época de verano en la costa central del Perú (Lima), con temperatura ambiental promedio de 25°C. Codornices reproductores (270 hembras y 90 machos) de 48 días de edad, fueron distribuidas en cinco tratamientos (4 con aditivos y una dieta control) con tres rep...

  15. Growth performance, meat quality traits, and genetic mapping of quantitative trait loci in 3 generations of Japanese quail populations (Coturnix japonica).

    Science.gov (United States)

    Tavaniello, S; Maiorano, G; Siwek, M; Knaga, S; Witkowski, A; Di Memmo, D; Bednarczyk, M

    2014-08-01

    The current research was conducted to compare growth, carcass traits, pH, intramuscular collagen (IMC) properties, and genetic bases of IMC and carcasses (breast-muscle weight) of different lines and generations of adult males and females of Japanese quail (Coturnix japonica). Forty-four quails (generation F0), 22 Pharaoh (F-33) meat-type males and 22 Standard (S-22) laying-type females, were crossed to produce the F1 hybrids generation. The F2 generation was created by mating one F1 male with one F1 female, full siblings. The birds, randomly chosen from F0 (22 males and 22 females), F1 (22 males and 22 females), and F2 (84 males and 152 females) were raised to 20 wk of age in collective cages. Quails were fed ad libitum commercial diets. At slaughter, all birds were individually weighed (after a fasting period of 12 h) and dressing yield (without giblets) was calculated. The carcasses were then dissected. Genomic DNA was extracted from all of the blood, and 30 microsatellite markers located on 2 quail chromosomes were genotyped. The F -: 33 quails had higher in vivo and postmortem performances and a higher abdominal fat percentage than those of the egg line. Meat from S -: 22 quails had a slower collagen maturation (hydroxylysylpyridinoline crosslink/collagen) and a higher ultimate pH. The F1 and F2 generations showed an evident sexual dimorphism, and an additional effect could be due to hybrid heterosis evident in F2. Meat from quails of F1 and F2 generations had a lower IMC amount with a higher degree of collagen maturation compared with parental lines. Two statistically significant QTL have been detected on quail chromosome 2 (CJA02): a QTL with an additive effect (0.50) for IMC in the marker bracket GUJ0037 and GUJ0093; a second QTL with additive (1.32) and dominant (1.91) effects for breast-muscle weight in the marker bracket GUJ0084 and GUJ0073. To our knowledge, this is the first report of a QTL associated with breast-muscle weight and IMC in quail and

  16. ( Coturnix coturnix japonica ) fed processed sweet potato ( Ipomea ...

    African Journals Online (AJOL)

    A six–week feeding trial was carried out to investigate the effect of processing of sweet potato tuber on growth parameters and carcass values of Japanese quails. Five isonitrogenous (25%CP) diets were compounded. The control diet (A) had zero sweet potato tuber meal. The other four diets (B, C, D and E) contained ...

  17. Effects of irradiated barley on fattening quail (Coturnix coturnix japonica)

    International Nuclear Information System (INIS)

    Dahlhelm, H.

    1999-01-01

    For the feeding experiments reported, barley grains irradiated at doses of 2, 10, and 100 kGy were used as a diet. The results obtained revealed no significant effects in the parameters analysed. (orig./CB) [de

  18. Análise clínica, radiológica, macroscópica e histológica do úmero de codornas domésticas (Coturnix japonica, submetido ao implante da poliuretana derivada do polímero de mamona (Ricinnus communis Clinical, radiological, macroscopical and histological analysis of domestic quail (Coturnix japonica humerus submitted to implant of polyurethane from castor oil polymer (Ricinnus communis

    Directory of Open Access Journals (Sweden)

    Juliano Bolson

    2005-10-01

    Full Text Available Na cirurgia ortopédica, são freqüentes as situações em que o cirurgião se depara com grandes perdas ósseas, provocadas especialmente por traumatismos de alta energia, tumores ou infecções. A reparação dessas fraturas requer grande conhecimento a respeito de materiais que possam ser utilizados para preencher essas falhas. Esses materiais podem ser derivados biológicos, sintéticos ou metálicos. Dentre eles, destacam-se os enxertos ósseos e os implantes de biomateriais. Com o aumento dos animais exóticos, utilizados como animais de estimação, a casuística clínica e cirúrgica dessa especialidade vem crescendo, tanto em clínicas quanto em hospitais veterinários. Particularmente, as aves constituem a maioria desses animais, e entre seus problemas cirúrgicos as fraturas são os de maior destaque. O objetivo deste trabalho foi estudar o efeito clínico, radiológico, macroscópico e histológico da poliuretana derivada do polímero de mamona (Ricinnus communis implantada no úmero de codornas domésticas (Coturnix japonica. Para isso, foram utilizadas 20 codornas, machos e fêmeas, separadas aleatoriamente em quatro grupos de cinco animais, os quais receberam o implante no úmero esquerdo, com exame clínico diário durante o período pós-operatório, avaliações radiológica imediata e quinzenal e macroscópica e histológica aos 15, 30, 60 e 90 dias. Clinicamente não foram observadas alterações locais, regionais nem sistêmicas. Ao exame radiológico, notou-se aumento de densidade local, sem sinais de alteração óssea, dos tecidos circunjacentes, e de sacos aéreos. A análise macroscópica revelou que a poliuretana de mamona não foi absorvida em nenhum dos quatro grupos permanecendo implantada no seio do osso pneumático, porém, notou-se alteração em sua resistência. O exame histopatológico mostra inicialmente mínima reação inflamatória, discreta fibrose ao redor do implante e osteointegração pela presen

  19. Determining population trends and conservation status of the common quail (Coturnix coturnix in Western Europe

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    Puigcerver, M.

    2012-01-01

    Full Text Available In this paper we review the conservation status and population trends of the common quail (Coturnix coturnix from 1900 to the present. Data are sometimes contradictory with regard to the status of this species as it has some features that make it difficult to produce reliable population estimates. Recent data clearly suggest, either at a local scale or at a trans–national scale, that the Atlantic common quail populations have remained stable in the last two decades, and that restocking practices with farm–reared quails (hybrids with the Japanese quail, Coturnix japonica do not affect our estimates. The complex movement patterns showed by this species require special attention. Analysis of ring recoveries can give important information, especially about the nomadic movement of quails in search of suitable habitats after the destruction of winter cereal crops due to harvesting. Thus, when developing a breeding distribution model for this species, continuously updated information on seasonal habitat and weather must be included for optimal prediction. Including fortnightly data of vegetation indices in distribution models, for example, has shown good results. Obtaining reliable predictions about changes in species distribution and movements during the breeding period could provide useful knowledge about the conservation status and population trends and would help in the design of future management measures.

  20. Pathobiology and transmission of highly and low pathogenic avian influenza viruses in European quail (Coturnix c. coturnix).

    Science.gov (United States)

    Bertran, Kateri; Dolz, Roser; Busquets, Núria; Gamino, Virginia; Vergara-Alert, Júlia; Chaves, Aida J; Ramis, Antonio; Abad, F Xavier; Höfle, Ursula; Majó, Natàlia

    2013-03-28

    European quail (Coturnix c. coturnix) may share with Japanese quail (Coturnix c. japonica) its potential as an intermediate host and reservoir of avian influenza viruses (AIV). To elucidate this question, European quail were experimentally challenged with two highly pathogenic AIV (HPAIV) (H7N1/HP and H5N1/HP) and one low pathogenic AIV (LPAIV) (H7N2/LP). Contact animals were also used to assess the viral transmission among birds. Severe neurological signs and mortality rates of 67% (H7N1/HP) and 92% (H5N1/HP) were observed. Although histopathological findings were present in both HPAIV-infected groups, H5N1/HP-quail displayed a broader viral antigen distribution and extent of microscopic lesions. Neither clinical nor pathological involvement was observed in LPAIV-infected quail. Consistent long-term viral shedding and effective transmission to naive quail was demonstrated for the three studied AIV. Drinking water arose as a possible transmission route and feathers as a potential origin of HPAIV dissemination. The present study demonstrates that European quail may play a major role in AI epidemiology, highlighting the need to further understand its putative role as an intermediate host for avian/mammalian reassortant viruses.

  1. Energia metabolizável de ingredientes determinada com codornas japonesas (Coturnix coturnix japonica Metabolizable energy of feedstuffs determined in japanese quails (Coturnix coturnix japonica

    Directory of Open Access Journals (Sweden)

    José Humberto Vilar da Silva

    2003-12-01

    Full Text Available O experimento um foi realizado para determinar a energia metabolizável aparente (EMA e corrigida pelo balanço de nitrogênio (EMAn de nove alimentos utilizando codornas japonesas em crescimento. No experimento dois, objetivou-se comparar formulações de rações utilizando EMAn do milho e do farelo de soja determinada para frangos de corte e poedeiras, com aquelas determinadas com codornas com 22 a 27 dias de idade e 65 dias de idade. No experimento um, foram utilizadas 400 codornas em crescimento recebendo uma dieta basal (DB e nove misturas compostas por 70% da DB + 30% dos alimentos testes, totalizando dez tratamentos, cada um com quatro repetições de dez aves. No experimento dois, 160 codornas européias em postura receberam três tratamentos durante três períodos de 15 dias de duração, com doze repetições de cinco aves. Os valores de EMA e EMAn (kcal/kg determinados para os alimentos de origem vegetal foram, respectivamente, 3.340 e 3.354 para o milho moído, 2.718 e 2.456 para o farelo de soja, 3.453 e 3.084 para a soja integral extrusada, 1.624 e 1.593 para o farelo de trigo, 4.558 e 3.992 para o farelo de glúten de milho, 3.329 e 3.378 para a farinha de mandioca e 1.238 e 1.223 para a farinha integral da vagem de algaroba e para os alimentos de origem animal, respectivamente, de 2874 e 2453 para a farinha de peixe e 3090 e 2791 para a farinha de vísceras. A EMAn do milho e do farelo de soja estimada com codornas não melhorou o consumo, produção, peso e conversão por massa de ovos, validando o uso da energia desses ingredientes determinada com frangos de corte e poedeiras para compor rações para codornas.The experiment one was carried out to determine apparent metabolizable energy (AME and nitrogen-corrected ME (AMEn of nine feedstuffs in Japanese quails. The objective of the experiment two was to compare diets formulated with AMEn of corn and soybean meal, usually fed to broilers and laying hens, with diets formulated with AMEn determined in growing (22 days of age and adult quails (65 days of age. In the experiment one, 400 growing quails were fed a basal diet (BD and nine test diets (70% BD + 30% feedstuffs test, with a total of ten diets with four replicates of ten birds each. In the experiment two, 160 European quails were randomized allotted to three treatments, with twelve replicates of five birds, and fed during three periods of fifteen days. The AME and AMEn values (kcal/kg for vegetal feedstuffs were: 3,340 and 3,354 for corn, 2,718 and 2,456 for soybean meal, 3,453 and 3,084 for integral soybean extruded, 1,624 and 1,593 for wheat bran, 4,558 and 3,992 for corn gluten meal, 3,329 and 3,378 for cassava flour and 1,238 and 1,223 to integral mesquite pods meal. The animal feedstuffs had 2,874 and 2,453 for fish meal and 3,090 and 2,791 for poultry meal. The AMEn of corn and soybean meal estimated for quail did not improve feed intake, egg production, egg weight and feed egg mass ratio, supporting the validity of use of energy those ingredients obtained with broiler and laying hens for formulated quail diets.

  2. Quail egg yield and quality of the Coturnix coturnix response to the addition level of agave inulin to the drinking water

    Directory of Open Access Journals (Sweden)

    Luis Jorge Pérez de la Mora

    2014-05-01

    Full Text Available To assess the effect of agave inulin addition to drinking water, one hundred (65 days old Coturnix coturnix japonica hens were divided randomly into four groups with five birds per cage. Twenty five birds were assigned to each of the following treatments: i control; addition of agave inulin in the drinking water at; ii 2.0% (w/w, iii 4.0%; and iv 6%. The feeding trial lasted for six weeks. Inulin increased (P0.05. The egg weight was similar among treatments (average 12.70 g and varied slightly with the inulin level (P=0.0537. The egg shell (percentage of the egg was not influenced by the inulin. But, the calcium concentration in the eggshell of the present trial varied with the level on the agave inulin in the drinking water (P0.05. Then, the agave inulin addition to the drinking water increases the egg yield of Japanese quails.

  3. Variance components for body weight in Japanese quails (Coturnix japonica

    Directory of Open Access Journals (Sweden)

    RO Resende

    2005-03-01

    Full Text Available The objective of this study was to estimate the variance components for body weight in Japanese quails by Bayesian procedures. The body weight at hatch (BWH and at 7 (BW07, 14 (BW14, 21 (BW21 and 28 days of age (BW28 of 3,520 quails was recorded from August 2001 to June 2002. A multiple-trait animal model with additive genetic, maternal environment and residual effects was implemented by Gibbs sampling methodology. A single Gibbs sampling with 80,000 rounds was generated by the program MTGSAM (Multiple Trait Gibbs Sampling in Animal Model. Normal and inverted Wishart distributions were used as prior distributions for the random effects and the variance components, respectively. Variance components were estimated based on the 500 samples that were left after elimination of 30,000 rounds in the burn-in period and 100 rounds of each thinning interval. The posterior means of additive genetic variance components were 0.15; 4.18; 14.62; 27.18 and 32.68; the posterior means of maternal environment variance components were 0.23; 1.29; 2.76; 4.12 and 5.16; and the posterior means of residual variance components were 0.084; 6.43; 22.66; 31.21 and 30.85, at hatch, 7, 14, 21 and 28 days old, respectively. The posterior means of heritability were 0.33; 0.35; 0.36; 0.43 and 0.47 at hatch, 7, 14, 21 and 28 days old, respectively. These results indicate that heritability increased with age. On the other hand, after hatch there was a marked reduction in the maternal environment variance proportion of the phenotypic variance, whose estimates were 0.50; 0.11; 0.07; 0.07 and 0.08 for BWH, BW07, BW14, BW21 and BW28, respectively. The genetic correlation between weights at different ages was high, except for those estimates between BWH and weight at other ages. Changes in body weight of quails can be efficiently achieved by selection.

  4. Condition-dependent strategies of eggshell pigmentation: an experimental study of Japanese quail (Coturnix coturnix japonica)

    Czech Academy of Sciences Publication Activity Database

    Duval, C.; Cassey, P.; Mikšík, Ivan; Reynolds, S.J.; Spencer, K.A.

    2013-01-01

    Roč. 216, č. 4 (2013), s. 700-708 ISSN 0022-0949 Institutional support: RVO:67985823 Keywords : body condition * eggshell colouration * food restriction * pigment allocation Subject RIV: EG - Zoology Impact factor: 3.002, year: 2013

  5. Comparative metabolism and fate of fenvalerate in Japanese quail (Coturnix coturnix japonica) and rats (Rattus norwegicus)

    International Nuclear Information System (INIS)

    Mumtaz, M.M.; Menzer, R.E.

    1986-01-01

    Adult Japanese quail were administered 100 mg/kg chlorophenyl-labeled [ 14 C]fenvalerate, α-cyano-3-phenoxybenzyl 2-(4-chlorophenyl)isovalerate, for study of its distribution, elimination, and metabolism. Ninety percent of the administered dose was eliminated in the excreta within the first 24 h. In addition to fenvalerate, the following metabolites were present: benzeneacetic acid, 4-chloro-α-(1-methylethyl)-,cyano(3-phenoxy-4-hydroxyphenyl)methyl ester [4'-OH-fenvalerate]; benzeneacetic acid, 4-chloro-α-(1-methylethyl)-,(aminocarbonyl)(3-phenoxyphenyl)methyl ester [CONH 2 -fenvalerate]; 4-chloro-α-(1-methylethyl)benzeneacetic acid[Cl-V acid]; 4-chloro-α-(2-hydroxy-1-methylethyl)benzeneacetic acid[4-OH-Cl-V acid]. In time course studies radiocarbon peaked at 3 h (9μg/g) in the liver and gradually declined, while in the blood it peaked within 2 h and fell quickly to an equilibrium value of 1.5 μg/mL blood. In liver microsomal and isolated heptatocyte preparations of Japanese quail and rat, the following metabolites were identified: Cl-V acid, 4-OH-Cl-V acid, 4'-OH-fenvalerate, CONH 2 -fenvalerate. Oxidation was found to be the predominant route of degradation either pre- or post-hydrolysis of the parent compound. Rapid excretion, lesser absorption, and faster metabolism probably explain the lower toxicity of fenvalerate to birds compared to rats

  6. Performans produksi burung puyuh (Coturnix coturnix japonica) dengan perlakuan tepung limbah penetasan telur puyuh

    OpenAIRE

    Lukluil Maknun; Sri Kismiati; Isna Mangisah

    2015-01-01

    The research aimed to evaluate the utilization of Japanese quail hatchery waste powder in feed on feed intake, egg production, egg mass and feed conversion ratio. The materials used were 4 weeks old 160 Japanese quails with an average weight of 94.19 ± 7.36 gram. The experiment used a completely randomized design (CRD) with four treatments (0%, 9%, 12%, and 15% of Japanese quail hatchery waste powder in feed) and five replications. Parameters observed were feed intake, egg production, egg mas...

  7. DETERMINATION OF OPTIMUM THREONINE REQUIREMENTS OF JAPANESE QUAIL (Coturnix coturnix japonica CHICKS REARED UNDER TROPICAL ENVIRONMENT

    Directory of Open Access Journals (Sweden)

    Isaac Samuel

    2017-12-01

    Full Text Available Threonine, like most amino acids, is traditionally noted for its role in protein synthesis. However, dietary threonine concentration required for the maximum performance of Japanese quails is yet to be determined, therefore, a study was conducted to determine the optimum threonine requirement of Japanese quail chicks in the tropical environment. A total of four hundred and fifty, two weeks old quail chicks (mixed sexes were randomly allocated to five dietary treatments (0.67, 0.81, 0.95, 1.08 and 1.22 % total threonine and replicated thrice in a completely randomized design (CRD. There were no significant differences (P>0.05 in average weight gain, average feed intake, feed conversion ratio and age at first lay of quails fed the dietary treatments. Weight of first egg laid was significantly (P0.05 similar for all treatments. There were significant differences (P0.05. It was concluded that the performance of birds were not influenced by the dietary treatments up to the highest dietary level of threonine studied (1.22%. Therefore, it is possible that the optimum dietary level of threonine may be above 1.22 %. Further studies will be necessary to establish higher doses of dietary threonine requirement of Japanese quails reared under tropical climatic environment.

  8. The distribution of 14C-chloramphenicol in the Japanese quail (coturnix coturnix japonica)

    International Nuclear Information System (INIS)

    Appelgreen, L.-E; Biessmann, A.; Martin, K.; Salemi, M.A.; Wiese, B.

    1985-01-01

    The distribution of 14 C-labelled chloramphenicol after oral and intravenous administration to egg laying Japanese quail was studied by whole-body autoradiography. In the liver, kidneys, gizzard, intestinal contents (bile) and oviduct, the 14 C-concentration was higher than that of the blood short time after injection and remained higher than the blood up to 4 days. From 4 hrs, the concentration of 14 C in the egg yolks was higher than that of the blood and from 24 hrs the radioactivity in the albumen of the eggs in the oviduct was also higher than that of the blood. The peak concentration in the egg yolk was found in the second egg laid 2-4 days after administration of 14 C-chloramphenicol. In the albumen the maximum concentration was found in the first laid egg 24-48 hrs after administration. In the egg yolks, about 30% of the radioactivity represented unchanged chloramphenicol up to 5 days after administration. It was also shown that about 5% of the injected 14 c-chloramphenicol was exhaled as 14 CO 2 during the first 12 hrs and about 37% of the dose was excreted in the combined faeces and urine during the same period of time. (author)

  9. Incorporation of radioactive zinc into the eggs of Japanese quails (coturnix coturnix Japonica)

    International Nuclear Information System (INIS)

    Matsusaka, Naonori; Nishimura, Yoshikazu; Nakamura, Isao; Yuyama, Akira

    1975-01-01

    The incorporation of 65 Zn into eggs was investigated after single or daily intraperitoneal administration to laying Japanese quails. After a single intraperitoneal administration, 65 Zn appeared first in the 2nd egg yolks and reached the highest level in the 3rd and 4th ones, followed by a gradual decrease. The cumulative amount of 65 Zn in the egg yolks over a period of 3 weeks accounted for about 40% of dose. After daily intraperitoneal administration, the 6th-9th egg's yolks contained the highest radioactivity of the eggs examined. Whole-body retention patterns were also observed in both of the experiments. (auth.)

  10. Sex Determination of Japanese Quails (Coturnix Coturnix Japonica using with Zoometric Measurements

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    Tülin Çiçek Rathert

    2017-09-01

    Full Text Available The difficulty of sex determination in most poultry species causes significant financial losses for poultry production as birds cannot be separated at early stages of growth for meat or egg production. Therefore it is important to determine bird’s sex with zoometric parameters. This study was carried out to determine the sex of Japanese quails with zoometric measurements, such as live weight, body length, chest depth and chest width. Eighty-eight male and female Japanese quail chicks were used individually for live weight, chest depth (mm, chest width (mm and body length (mm with using digital scaled balance and caliper for every week over a period of six weeks. The weekly collected data were applied to t test for estimating the sex discrimination. The Pearson’s correlation was applied for examining the interrelationship between sex and biometric traits. The results indicated that there was a significant positive correlation between live weight and body length beginning with the 2nd week. Therefore, zoometric measurement of these body traits is suitable for discriminating the sex of Japanese quails in early phase of life.

  11. Nutrient digestibility of vegetables waste flour on male quail (Coturnix coturnix japonica)

    Science.gov (United States)

    Pramono, A.; Primadhani, M. S.; Swastike, W.; Sutrisno, J.

    2018-03-01

    The aim of this research is to determine the nutrient digestibility of vegetables waste flour on of male quail. Four hundred male quails were divided into four groups with five replications. The experiment is Completely Randomized Design and the data were analyzed by analyses of variants. The experimental diets were P0 = basal diet, P1 = 97% basal diet + 3% vegetables waste flour, P2 = 94% basal diet + 6% vegetables waste flour, and P3 = 91% basal diet + 9% vegetables waste flour. The observed variables were the digestibility of dry matter, crude protein and extract ether. Result showed that of the addition of vegetable waste flour in the diet had no effect on crude protein digestibility (P>0.05), however shown significant effect on dry matter (P digestibility.

  12. Pengaruh penggunaan tepung limbah udang fermentasi terhadap karakteristik organ reproduksi pada puyuh petelur (Coturnix coturnix japonica

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    Welda Hilkias

    2017-06-01

    Full Text Available The aim of the present study was to evaluate the effect of fermented shrimp waste meal on the characteristics of reproductive organs such as live weight, oviduct weight and length, ovarian weight, total yellow follicles, total white follicles, oviduct percentage and reproductive organ percentage. This experiment was conducted on Faculty of Animal Science and Agriculture, Diponegoro University. A total of 250, 6-week-old Japanese quail (consist of 250 females were randomly assigned to one of 5 dietary treatments. 1st group (T0 was fed by diet without shrimp waste (control, 2nd group (T1 was fed by diet with 7,5% non-fermented shrimp waste, 3rd group (T3 was fed by diet with 5% fermented shrimp waste (FSW, 4th group (T3 was fed by diet with 7,5% FSW and 5th group was fed by diet with 10% FSW. 2 quails from each replicates were taken as samples. The members of a given sample were killed by severing the neck then separated the reproductive organs from the body. Measured the live weight, oviduct weight and length, ovarian weight, total yellow follicles, total white follicles, oviduct percentage and reproductive organ percentage. All data were examined using analysis of variance (ANOVA. The results showed that the effect of fermented shrimp waste meal on reproductive organs characteristics such as live weight, oviduct weight and length, ovarian weight, total yellow follicles, total white follicles, oviduct percentage and reproductive organ percentage were not significant. The use of fermented shrimp waste up to 10% didn’t obstruct the growth and development of quail reproductive organs.

  13. Scanning Electron Microscopic Studies of the Pecten Oculi in the Quail (Coturnix coturnix japonica

    Directory of Open Access Journals (Sweden)

    Aris F. Pourlis

    2013-01-01

    Full Text Available The main purpose of this study is to extend the microscopic investigations of the pecten oculi in the quail in order to add some information on the unresolved functional anatomy of this unique avian organ. The pecten oculi of the quail was studied by scanning electron microscopy. Eighteen- to-twenty two highly vascularised accordion-like folds were joined apically by a heavily pigmented bridge of tissue, which holds the pecten in a fanlike shape, widest at the base. The structure of the double layered limiting membrane was recorded. The presence of hyalocytes with macrophage-like appearance was illustrated. It is assumed that the pecten oculi of the quail resembles that of the chicken. Illustrated morphological features of this species may add information on the active physiological role of the pecten. But still, the functional significance of this organ is a matter of controversies.

  14. The Effects of Hyperthyroidism and Hypothyroidism Over the Egg Quality in Japanese Quails (Coturnix coturnix Japonica)

    OpenAIRE

    ÖZCAN, Mukaddes; ÇÖTELİOĞLU, Ülker

    1998-01-01

    In this study, the influence of hyperthyroidism and hypothyroidism on the egg quality in 5 week-female Japanese quails was investigated. Hyperthyroidsm and hypothyroidism were achieved by mixing 0.4 mg/100 g. levatrocsyn-sodium and % 0.2 g. propylthiouracil (PTU) in to the food during the 5 weeks period, respectively. It was found that egg weight, albumen weight, shell weight and shell thickness values in both trial groups reduced (P

  15. Eriobotrya japonica

    African Journals Online (AJOL)

    user

    2011-03-28

    Mar 28, 2011 ... pyrophosphatase (V-PPiase) were isolated from loquat (Eriobotrya japonica) pulp. Thereafter, northern analysis ... #These authors contributed equally to this work. ... (TA) correlates highly with malate concentration, but does not correlate ... (1997) have shown that, a nonacid sweet lime (Citrus limmetioides) ...

  16. Pairing Behavior of the Monogamous King Quail, Coturnix chinensis.

    Directory of Open Access Journals (Sweden)

    Elizabeth Adkins-Regan

    Full Text Available Animals with socially monogamous mating systems are valuable for discovering proximate mechanisms of prosocial behavior and close social relationships. Especially powerful are comparisons between related species that differ in monogamous tendency. Birds are the most socially monogamous vertebrates. Thus far most research on mechanisms of pairing has used zebra finches, which do not have a relative with a different mating system, however. The goal of the experiments reported here was to develop a new comparative avian system by studying the pairing behavior of a reportedly strongly monogamous quail, the king quail (Coturnix chinensis, a species in the same clade as the less monogamous Japanese quail (Coturnix japonica, the subject of much prior research. In Experiment 1 male-female pairs of king quail housed together were initially avoidant or aggressive but most rapidly progressed to allopreening and huddling. A separation-reunion paradigm reliably elicited both of these behaviors in males that had cohabited for one week. In Experiment 2 the allopreening and huddling behavior of males in cohabiting pairs was highly selective, and a majority of the males were aggressive toward a familiar female that was not the cohabitation partner. In Experiment 3 males were separated from their female cohabitation partners for 9-10 weeks and then given two-choice tests. All but one male spent more time near an unfamiliar female, which may have reflected aggression and shows recognition of and memory for the past pairing experience. Thus king quail show robust, selective and easy to measure pairing behavior that can be reliably elicited with simple separation-reunion testing procedures. Copulation is rarely seen during tests. The behavior of king quail is a striking contrast to that of Japanese quail, providing a new comparative system for discovering mechanisms of behavior related to close social relationships and monogamy.

  17. Pairing Behavior of the Monogamous King Quail, Coturnix chinensis.

    Science.gov (United States)

    Adkins-Regan, Elizabeth

    2016-01-01

    Animals with socially monogamous mating systems are valuable for discovering proximate mechanisms of prosocial behavior and close social relationships. Especially powerful are comparisons between related species that differ in monogamous tendency. Birds are the most socially monogamous vertebrates. Thus far most research on mechanisms of pairing has used zebra finches, which do not have a relative with a different mating system, however. The goal of the experiments reported here was to develop a new comparative avian system by studying the pairing behavior of a reportedly strongly monogamous quail, the king quail (Coturnix chinensis), a species in the same clade as the less monogamous Japanese quail (Coturnix japonica), the subject of much prior research. In Experiment 1 male-female pairs of king quail housed together were initially avoidant or aggressive but most rapidly progressed to allopreening and huddling. A separation-reunion paradigm reliably elicited both of these behaviors in males that had cohabited for one week. In Experiment 2 the allopreening and huddling behavior of males in cohabiting pairs was highly selective, and a majority of the males were aggressive toward a familiar female that was not the cohabitation partner. In Experiment 3 males were separated from their female cohabitation partners for 9-10 weeks and then given two-choice tests. All but one male spent more time near an unfamiliar female, which may have reflected aggression and shows recognition of and memory for the past pairing experience. Thus king quail show robust, selective and easy to measure pairing behavior that can be reliably elicited with simple separation-reunion testing procedures. Copulation is rarely seen during tests. The behavior of king quail is a striking contrast to that of Japanese quail, providing a new comparative system for discovering mechanisms of behavior related to close social relationships and monogamy.

  18. Cuscuta japonica Choisy

    OpenAIRE

    2012-01-01

    The influence of the aqueous extract of Japanese Dodder (Cuscuta japonica) on the velocity of Mycobacterium tuberculosis growth has been shown. To accelerate the growth of Myc. tuberculosis, dry culture media of FAST-3L and of Lowenstein Jensen were mixed with the aqueous extract of Cuscuta japonica. It was found out that the complex chemical composition of the aqueous extract made of dry seeds or dry vegetative stems of the plant parasite Cuscuta japonica has a biological effect on Myc. tube...

  19. Celulitis in Japanese Quails (coturnix coturnix japonica for Eschorichia coli: virulence factors, sensibility and profile antimicrobial resistance /Celulite em codornas (coturnix coturnix japonica causada por Escherichia coli: fatores de virulência, sensibilidade e perfil de resistência antimicrobiana

    Directory of Open Access Journals (Sweden)

    Marilda Carlos Vidotto

    2000-12-01

    Full Text Available Ten E. coli strains isolated from celulitis lesion s of Japanese quails were to evaluated antimicrobia l resistance to twent y six drugs , to pathogenicity of strains in SPF chickens embryonated eggs and virulence factors. The antimicrobials of higher efficiency wer e ampicillin, florfenicol and the lesser efficiency were erythromycin, oxacilin, lincomicin, novobiocin, penicillin, sulfonamidas, trimethoprim+sulfomethoxazo/e and tetracyicline. The majority of E. coli strains were serum resistance, the others virulence factors, hemolisin and congo red affinity, were lesser frequent on the studied strains. Pathogenicity of E. coli strains, evaluated to DL50 in embryonated eggs, had varied of 8x10 2 the 3,2x10.Dez cepas de E. coli isoladas de lesões de celulite em codornas foram avaliadas quanto a resistência antimicrobiana frente a vinte e seis drogas, a patogenicidade das amostras em ovos embrionários de galinha SPF e quanto aos fatores de virulência: hemolisinas, resistência sérica e afinidade ao vermelho congo Os antimicrobianos de maior eficiência foram ampicilinar florfenicol e os menos eficientes foram eritromicina, oxacilina. lincomicina, novobiocina. penicsilna, sulfonamida, sulfomethoxazole+ trimetoprim e tetraciclina. A maioria das amostras de E. coli foram resistentes ao soro, os outros fatores do virulência, hemolisina e afinidade ao vermelho-congo, foram menos freqüentes nas amostras estudadas. A patogenicidade das amostras de E. coli estimada através da DL50 em ovos embrionados, variaram de 8x10* a 3.2x10a.

  20. Physiological and Biochemical Responses in Japanese Quail (Coturnix coturnix japonica) Fed Radiation Processed Aflatoxin-Contaminated Diet

    International Nuclear Information System (INIS)

    El-Niely, H.F.G.; Abdalla, E.A.; Abd El-Azeem, A.F.

    2008-01-01

    Aflatoxins (AFs) contamination of foods and live-stock feeds is an ongoing problem. In this research, the amelioration of aflatoxicosis in Japanese quails was examined by the radiation processing of their contaminated-diets, as a physical detoxifying method. Diets contaminated with two different levels of AFB 1 (2 or 5 mg kg -1 diet) were subjected to 10, 20, or 30 kGy and fed to growing Japanese quails for 5 weeks. The physiological and biochemical responses were evaluated for irradiation ability to reduce the deleterious effects of 2 and 5 mg AFB 1 kg -1 diet. A total of 270 seven-day-old Japanese quail chicks were assigned to 2 factorial arrangements of nine treatments (level of toxin and radiation dose) each consisted of three replicates with 10 quails per pen. The significant adverse effect of AFB 1 on the food consumption, body-weight gain, food conversion ratio, mortality rate and internal organ weights, from the first week onwards, were determined. Radiation processing reduced concentration of AFB 1 in all experimental diets and significantly reduced the deleterious effects of AFB 1 on food consumption, body-weight gain, food conversion ratio, and the relative weights of most observed organs, as a function of radiation dose. Muscles, liver, kidneys and heart tissues were analyzed for aflatoxin (AF) residues. The residual level of AFB 1 was significantly higher in liver than in kidneys, muscles or heart. The level in the observed organs and the muscles was lower in those received irradiated diet at high 30 kGy. Plasma samples were tested for glucose, triglyceride, cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), urea and creatinine. They were significantly increased in AF treated groups in comparison with those received AF-containing diet and irradiated up to 30 kGy. Birds ate contaminated diet with both level of toxin were suffered from the lower level of total proteins, albumin, globulin, phosphorus and calcium. A function of radiation doses the physiological and biochemical responses in quails fed irradiated AF-containing diets were significantly improved, 30-kGy radiation dose was more effective in this respect.The current study showed that the use of radiation processing in AF-contaminated feed (2 or 5 mg kg -1 diet), significantly reduced negative effects of AF on toxicity. This effect can be related to the change in the AF molecule structure by the irradiation treatment into less toxic forms. AFs contamination in birds feed may cause economic losses by lowering body wt gain. Therefore, lower levels of AFs in the bird feeds should be required if all acceptable risk is to be avoided. Additionally, the risk of AFs in birds as a food appears to remain very low, although the levels of AFs in human food should be kept as low as possible to reduce the incidence of hepatic cancer

  1. Carbon-14-ochratoxin A distribution in the Japanese quail (Coturnix coturnix japonica) monitored by whole body autoradiography

    International Nuclear Information System (INIS)

    Fuchs, R.; Appelgren, L.E.; Hagelberg, S.; Hult, K.

    1988-01-01

    Tissue distribution of the nephrotoxic mycotoxin ochratoxin A was characterized in laying Japanese quail by whole body autoradiography and scintillation counting using 14 C-labelled toxin. Periodically for 8 days after one intravenous injection of 14 microCi/bird, corresponding to 70 ng/g body weight, birds were killed, frozen, and sagittal sections of the whole body were placed on X-ray film. In general, the ochratoxin disappeared from the avian body rapidly. Specific retention of radioactivity was seen as a ring-like distribution in yolks and growing follicles. After sectioning, organs and intestinal contents were removed from carcasses in a frozen condition, homogenized, extracted, chromatographed, and the radioactivity in fractions was measured by scintillation spectroscopy. High concentrations of ochratoxin A were found in gastric intestinal contents, probably originating from toxin excreted in the bile

  2. Somatic and genetic effects of gamma radiation on Japanese quail (Coturnix coturnix japonica) and hen (Gallus gallus domestica)

    International Nuclear Information System (INIS)

    Baumgartner, J.

    1982-01-01

    Genetic changes were studied in economically significant species of birds and the impact of gamma radiation on their organisms in general. The impact is dealt with of small, medium and high doses of radiation on gonadal cells in vivo and in vitro and on fertilized eggs, and the total impact on the organism. During the irradiation of embryos toxic effects were unambiguously found for exposures to more than 100 R. The embryo was most sensitive between the 2nd and 4th day of development. No cytotoxic effect of radiation was observed in sperms exposed in vitro to less than 10,000 R. Irradiation of gonads affected the reproduction capacity of male birds substantially more than that of female birds. (M.D.)

  3. Immunocytochemical distribution of glial fibrillary acidic protein in the central nervous system of the Japanese quail (Coturnix coturnix japonica).

    Science.gov (United States)

    Cameron-Curry, P; Aste, N; Viglietti-Panzica, C; Panzica, G C

    1991-01-01

    In the present study we detailed the distribution of GFAP-immunopositive structures within the central nervous system of the Japanese quail. Different fixation and embedding procedures were applied. The best results were obtained on frozen cryostatic sections from freshly dissected brains subsequently fixed by a short immersion in cold acetone. Immunopositive structures were observed both with immunofluorescence, and with immunoperoxidase methods. Immunoreactive cell bodies and processes were observed within the whole central nervous system, and different cell types can be identified on the basis of their topographical location and morphology. A first class of astrocytes is composed of intensely stained unipolar cells lining the inner surface of the pia mater and the large blood vessels. A second type is represented by multipolar astrocytes of variable size, provided with an irregular cell body. The last type is represented by similar elements, showing an immunonegative cell body, that can be identified only by the presence of converging processes. These three types of cells, and several isolated processes, show a differential distribution within the quail central nervous system, both in the grey and in the white matter. Present results suggest that GFAP may represent a good marker for at least part of the astroglial population in quail.

  4. The positive expression of genotype VII Newcastle disease virus (Malaysian isolate in Japanese quails (Coturnix coturnix japonica

    Directory of Open Access Journals (Sweden)

    Lizma Felisha Mazlan

    2017-05-01

    Full Text Available Aim: Genotype VII Newcastle disease virus (NDV is the most predominant NDV strains that circulating in Malaysia; thus, this study was aimed to determine the susceptibility of Japanese quails toward genotype VII NDV. Clinical signs, gross pathological lesions of organs, positive detection of virus in organs and cloacal swabs, as well as the expression of the antibody titer, were used as parameters to assess the susceptibility of Japanese quails following infection of genotype VII NDV. Materials and Methods: About 20 quails were divided into three groups (n=8 for Groups A and B; n=4 for the control group. The quails in the Groups A and B were infected via intraocular route with 0.03 ml of 103.5 ELD50 and 107.0 ELD50 of NDV strain IBS 002, respectively, while the control group received 1x phosphate-buffered saline. Cloacal swabs and necropsy were taken on day 7 post-infection for all quails were subjected to one-step reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR for detection of virus and examination for gross pathological lesion, respectively. Blood serums of infected quails were taken on day 10, 14, and 21 post-day infections and were subjected for hemagglutination inhibition (HI assay. Results: Depression and ruffled feathers, trachea rales, leg paralysis, and torticollis were shown in some of the quails in both infected groups. Based on statistical analysis, there was no significant difference (p>0.05 in clinical signs between the infected groups. The results for RT-qPCR were found to be negative for all groups, and no gross pathological lesions of organs observed for quails in both infected groups. Trachea, proventriculus, and cecal tonsil were taken for the detection of NDV by RT-qPCR, and some of the organ samples showed positive detection of virus in both infected groups. HI assay showed an increase in mean titers of antibody across time and between infected groups. Conclusion: In summary, Japanese quails are susceptible to genotype VII NDV based on parameters assessed.

  5. The effect of mustard seed meal (Sinapis arvensis on thyroid hormones and liver enzymes in Japanese quails (Coturnix coturnix japonica

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    Sajjad Mohebali

    2016-07-01

    Full Text Available Objective: To investigate the effect of wild black mustard seed meal on thyroid hormones (thyroxine and thyroid-stimulating hormone in Japanese quails and also study the ability of FeSO4 to alleviate the possible negative effect of mustard meal on thyroid hormones in these birds for the first time. Methods: The experimental procedure was undertaken on 28 quails which were randomly assigned to a control and 6 test groups with 4 quails in each group for 28 days, during which the control group received basic diet with no mustard meal whereas the test groups (No. 2, 3 and 4 received mustard meal (5%, 10% and 15%, respectively and test groups (No. 5, 6 and 7 received FeSO4 (1%-treated mustard meal (5%, 10% and 15%, respectively on the basic of basic diet. Results: The group fed on 15% non-treated mustard seed meal had the least thyroxine level and its level backed to normal in group fed on 15% FeSO4-treated mustard seed meal although this group had the highest alanine transaminase and aspartate transaminase levels. Conclusions: We concluded that up to 10% FeSO4 mustard seed meal could be incorporated in the quail diet successfully with the least damage to thyroids and livers, but further investigations on these birds are still needed to confirm this hypothesis.

  6. Inorganic and organic mercury chloride toxicity to Coturnix: Sensitivity related to age and quantal assessment of physiologic responses

    Science.gov (United States)

    Hill, E.F.

    1982-01-01

    The toxicities of mercuric chloride (HgCl(,2)) and methylmercuric chloride (CH(,3)HgCl) were compared for coturnix (Coturnix coturnix japonica) from hatching to adulthood. Comparisons were based on: (1) Median lethal dosages (LD50) derived by administering single peroral and single intramuscular dosages of mercury, (2) median lethal concentrations (LC50) derived by feeding mercury for 5 days, (3) median toxic concentrations (TC50) derived by feeding mercury 9 weeks and measuring plasma enzyme activity, plasma electrolytes, and other blood constituents, and (4) transient changes of various blood chemistries following a single peroral dose of mercury. Acute peroral and intramuscular LD50s for HgCl(,2) and CH(,3)HgCl increased by two- to threefold for coturnix chicks from hatching to 4 weeks of age. Concomitantly, the LC50s also increased, but the important difference between test procedures was that with both single dose routes of exposure the toxicity ratios, i.e., HgCl(,2)/CH(,3)HgCl, at each age were about 2 to 2.5 compared to about 100 for the LC50s. For example, at 2 weeks of age the peroral LD50s for HgCl(,2) and CH(,3)HgCl were 42 and 18 mg/kg; the dietary LC50s were 5086 and 47 ppm for HgCl(,2) and CH(,3)HgCl. The 9 week feeding trial was not associated with gross effects from either HgCl(,2) at 0.5 to 32 ppm or CH(,3)HgCl at 0.125 to 8 ppm. However, subtle responses were detected for the plasma enzymes aspartate aminotransferase, lactate dehydrogenase, and ornithine carbamoyl transferase and could be quantified by probit analysis. This quantal procedure was based on establishment of a normal value for each enzyme and classing outliers as respondents. A 'hazard index' based on the TC50 for an enzyme divided by the LD50 or LC50 was introduced. The single oral dosages of HgCl(,2) and CH(,3)HgCl showed that ratios of alanine aminotransferase, lactate dehydrogenase, and orinthine carbamoyl transferase for the liver and kidneys of adult coturnix were opposite from

  7. Coturnix coturnix

    African Journals Online (AJOL)

    SARAH

    : The aim of this study was determine the effect of commercial feed of laying hens, principal feed .... durant l'essai ont varié respectivement de 20 à 30°C et ... Calcium / Phosphore. 2,0 ..... liver, meat and perirenal fat in rabbit.

  8. Lighting programs for male and female meat quails (Coturnix coturnix) raised in equatorial region.

    Science.gov (United States)

    Aguiar, G C; Freitas, E R; Watanabe, P H; Figueiredo, C W S; Silva, L P; Nascimento, G A J; Lima, R C; Nepomuceno, R C; Sá, N L

    2017-09-01

    The aim of the present study was to evaluate the effect of lighting programs for male and female meat quails raised in an equatorial region on performance and carcass traits. A total of 1,500 sexed quails (Coturnix coturnix) was distributed in a completely randomized design in a 2 × 3 factorial arrangement, with 2 sexes (male and female) and 3 lighting programs (natural, intermittent, and continuous) with 5 replicates of 50 birds. The lighting programs were applied in the period from 7 to 49 d of age, being natural (12 h and 30 min of natural light and 11 h and 30 min of dark), intermittent (12 h and 30 min of natural light and 5 h and 30 min of artificial light, alternating lighting periods with one h and 06 min and periods of darkness with one h and 12 min), and continuous (23 h of natural + artificial light and one h of dark). Regardless of lighting program, female quails were heavier than males, and birds subjected to natural light weighed less than those exposed to continuous and intermittent lighting programs. Quails exposed to intermittent and continuous light presented higher weight gain and feed intake, and worse feed conversion ratio compared to birds under a natural light regimen. It is concluded that in an equatorial region, it is possible to use a lighting program with only natural light for raising male and female meat quails from 7 to 49 d of age. © 2017 Poultry Science Association Inc.

  9. Neuroanatomical localization of endocrine control of reproductive behavior in the Japanese quail (Coturnix japonica)

    International Nuclear Information System (INIS)

    Watson, J.T. III.

    1989-01-01

    Steroid autoradiography and systematic and intracranial steroid treatment were undertaken to determine the neuroanatomical loci which are sufficient to activate steroid sensitive behaviors in the Japanese quail. (1) Autoradiographic localization of steroid binding cells was performed on male and female quail brains using tritiated ( 3 H) testosterone (T), estradiol (E2), or 5α-dihydrotestosterone (DHT). The distributions of labelled cells in the quail brain following 3 H-T or 3 H-E2 injection and autoradiography were similar to one another. The distribution of labelled cells following 3 H-DHT autoradiography was limited in comparison to that following 3 H-T autoradiography. Males were found to have more labelled cells than females in nucleus taeniae. (2) Intracranial implantation of minute pellets of testoterone propionate (TP) and estradiol benzoate (EB) was performed to determine neuroanatomical loci at which steroids activate sexual behavior. Both TP and EB implants in the preoptic area (POA) activated male copulatory behavior. (3) Systematic injection of aromatase inhibitor prior to and concurrent with implantation completely blocked copulatory behavior in males with TP implants in the POA but failed to block copulation in males with EB implants in the POA. (4) Intact males and castrated males given 5 dosages of systematic EB treatment were tested for sexual behavior, and blood samples from each group were assayed for E2 concentration. (5) Midbrain DHTP implants were activated crowing without significantly stimulating peripheral androgen-sensitive tissues, but the effect on crowing was not localized to any one nucleus

  10. Effect of 17β-trenbolone on male and female reproduction in Japanese quail (Coturnix japonica)

    Science.gov (United States)

    Henry, Paula F.P.; Akuffo, Valorie G.; Chen, Yu; Karouna-Renier, Natalie K.; Sprague, Daniel T.; Bakst, Murray R.

    2012-01-01

    The anabolic steroid 17β trenbolone (17β-TB), a known endocrine disrupting chemical, may influence reproductive functions in avian wildlife. We evaluated the effects of dietary exposure to 17β-TB at 5 and 20 ppm on reproductive functional endpoints in Japanese quail during and after sexual maturation. In the male, 5 and 20 ppm treatments revealed no differences in body and testes weight, testes histology, plasma testosterone concentrations, or size and weight of the foam glands. However, the onset of foam production was significantly earlier (days of age) in the 20 ppm males. In females, dietary 17β-TB at 20 ppm caused a reduction in the number of maturing yellow yolk follicles and overall egg production. Plasma testosterone concentrations were reduced compared to controls. Histology of the oviductal sperm storage tubules was normal in all treatments. The number of sperm holes, sites on the perivitelline layer (PVL) where sperm bound and hydrolyzed a path through the PVL, was significantly greater in the 10th egg laid compared to the 1st egg laid in the 20 ppm treatment. Potential effects, albeit transient, on endpoints associated with male maturation warrant further investigation into the sensitivity of these measures in the event of embryonic and/or trans-generational exposure to 17β-TB.

  11. Diagnostic Assay for Rickettsia japonica

    Science.gov (United States)

    Hanaoka, Nozomu; Matsutani, Minenosuke; Kawabata, Hiroki; Yamamoto, Seigo; Fujita, Hiromi; Sakata, Akiko; Azuma, Yoshinao; Ogawa, Motohiko; Takano, Ai; Watanabe, Haruo; Kishimoto, Toshio; Shirai, Mutsunori; Kurane, Ichiro

    2009-01-01

    We developed a specific and rapid detection system for Rickettsia japonica and R. heilongjiangensis, the causative agents of spotted fever, using a TaqMan minor groove binder probe for a particular open reading frame (ORF) identified by the R. japonica genome project. The target ORF was present only in R. japonica–related strains. PMID:19961684

  12. Complete nucleotide sequence of the Coturnix chinensis (blue-breasted quail) mitochondrial genome and a phylogenetic analysis with related species.

    Science.gov (United States)

    Nishibori, M; Tsudzuki, M; Hayashi, T; Yamamoto, Y; Yasue, H

    2002-01-01

    Coturnix chinensis (blue-breasted quail) has been classically grouped in Galliformes Phasianidae Coturnix, based on morphologic features and biochemical evidence. Since the blue-breasted quail has the smallest body size among the species of Galliformes, in addition to a short generation time and an excellent reproductive performance, it is a possible model fowl for breeding and physiological studies of the Coturnix japonica (Japanese quail) and Gallus gallus domesticus (chicken), which are classified in the same family as blue-breasted quail. However, since its phylogenetic position in the family Phasianidae has not been determined conclusively, the sequence of the entire blue-breasted quail mitochondria (mt) genome was obtained to provide genetic information for phylogenetic analysis in the present study. The blue-breasted quail mtDNA was found to be a circular DNA of 16,687 base pairs (bp) with the same genomic structure as the mtDNAs of Japanese quail and chicken, though it is smaller than Japanese quail and chicken mtDNAs by 10 bp and 88 bp, respectively. The sequence identity of all mitochondrial genes, including those for 12S and 16S ribosomal RNAs, between blue-breasted quail and Japanese quail ranged from 84.5% to 93.5%; between blue-breasted quail and chicken, sequence identity ranged from 78.0% to 89.6%. In order to obtain information on the phylogenetic position of blue-breasted quail in Galliformes Phasianidae, the 2,184 bp sequence comprising NADH dehydrogenase subunit 2 and cytochrome b genes available for eight species in Galliformes [Japanese quail, chicken, Gallus varius (green junglefowl), Bambusicola thoracica (Chinese bamboo partridge), Pavo cristatus (Indian peafowl), Perdix perdix (gray partridge), Phasianus colchicus (ring-neck pheasant), and Tympanchus phasianellus (sharp-tailed grouse)] together with that of Aythya americana (redhead) were examined using a maximum likelihood (ML) method. The ML analyses on the first/second codon positions

  13. Subchronic mercury exposure in coturnix and a method of hazard evaluation

    Science.gov (United States)

    Hill, E.F.; Soares, J.H.

    1984-01-01

    The sublethal toxicity of inorganic (HgCI 2) and organic (CH3HgCI) mercury chloride was studied in coturnix (Corurnix japonica) by feeding them mercuric compounds (CH3HgCI at concentrations of 0.125,0.5,2 and 8 ppm; HgCI2 at 0.5, 2, 8 and 32 ppm) in ad libitum diets from hatching to adulthood. Differences of response to the mercurials were compared on the basis of selected indicator enzymes and plasma chemistries. Comparisons of response to equivalent concentrations of the two mercurials and dose-response relationships were made at 1,3,5,7 and 9 weeks. Changes of activity were detected for brain acetylcholinesterase (AChE) and the plasma enzymes aspartate aminotransferase (ASA T), butyrylcholinesterase (BChE), lactate dehydrogenase (LDH) and ornithine carbamoyl transferase (OCT). Changes of ASA T, LDH and OCT were then quantified by probit analysis and the mercurials were compared through their median effective concentrations (EC50). This quantal procedure was based on the establishment of normal control values for each enzyme and then classifying mercury-treated outliers (more than + 2 SD) as respondents. The EC50 values at 9 weeks for ASA T, LDH and OCT, respectively, were 9, 3 and 63 ppm for HgCl 2, and 5, 1 and 4 ppm for CH3HgCI. These results provided the basis for two hazard indices that were calculated by dividing the EC50 into the oral LD50 and the 5-d dietary LC50. Mercury also had contradictory effects on gonadal maturation in both sexes.

  14. Supplementation of a Blend of Beneficial Bacteria and Antibodies on Growth Performance, Intestinal Mucosa Morphology and Right Heart Failure of Japanese Quail (Coturnix coturnix japonica

    Directory of Open Access Journals (Sweden)

    Mohammad Hasan Mehraei Hamzekolaei

    2017-05-01

    Full Text Available Background: Early nutrition of chicks with beneficial bacteria might help in occupying the inner surface of the intestinal tract. Interference of pathogens in intestinal microbiota is well known as barrier effect, bacterial interference, and competitive exclusion. Objectives: It was hypothesized that competitive exclusion in Japanese quails with a blend of beneficial bacteria (Aquablend Avian® probiotic would enhance quails’ growth performance and intestinal mucosal morphology. Furthermore, the study was performed at 2100 m above sea level at Shahrekord University, so another hypothesis was the capability of the probiotic for inhibiting right heart failure. Materials and Methods: One hundred fifty-six Japanese quails were divided into 4 groups: 2 groups (Aquablend and control at standard environmental temperature and 2 (Aqua-stress and Cont-stress at cold-hypoxic environmental situation. Aquablend groups received the probiotic in the first 3 days of life in drinking water (0.5 g/100 birds/day. Results: Feed conversion ratio (FCR was significantly reduced at the end of the experiment (day 35 in both Aquablend and aqua-stress groups compared to control and cont-stress groups, respectively (P 0.05. Cont-stress group had higher RV: TV ratio (0.28 and heterophil: lymphocyte (H: L ratio (1.22 than aqua-stress group: (0.25 and (1.20, respectively (P > 0.05. Data regarding to intestinal mucosa morphology was controversial but the probiotic was able to elevate duodenum villi surface (P < 0.05 and also jejunum and ileum lamina propria thickness. Conclusion: Obtained data suggests that addition of Aquablend Avian® probiotic in the first 3 days of life may improve growth performance and some intestinal mucosa characteristics of Japanese quails. Moreover, the probiotic might reduce right heart failure and stress induced by cold-hypoxic situation.

  15. Effects of dietary symbiotic supplementation on growth performance and duodenum histology of Japanese quail (Coturnix coturnix Japonica reared in different flooring systems

    Directory of Open Access Journals (Sweden)

    Isa Coskun

    Full Text Available ABSTRACT The objective of this study was to determine the effect of dietary symbiotic supplementation on the growth performance and duodenum histological parameters of quail reared in different flooring systems. A total of 160 mixed-sex healthy quail (47±0.32 g aged 14 days were used in a 2 × 2 factorial experimental design with four replicates, each including five males and five females. Two flooring systems (wire floor as control and wood shavings bedding and two dietary treatments (symbiotic supplementation and no supplementation were tested. Birds were transferred to four-tier cages for the trial. Each kilogram of the commercial diet included 224 g crude protein and 3,080 kcal metabolizable energy. The experiment lasted 21 days. Daily weight gain and gastrointestinal tract weight were statistically higher in the wood shavings and wood shavings + symbiotic-supplemented groups than in control group. Villi length was higher in the wood shavings + symbiotic and symbiotic-supplemented groups than in control and wood shavings groups. The wood shavings groups had longer villi than control group. Villi width was higher in wood shaving + symbiotic-supplemented groups compared with the other treatment groups. The litter system of wood shavings and symbiotic supplementation provide better growth performance to quail by developing their duodenum histomorphological parameters and digestive tract.

  16. CONTENT OF GLYCOGEN IN LIVER AND KETOBODIES IN BLOOD OF JAPANESE QUAILS (COTURNIX COTURNIX JAPONICA DURING STARVATION ENVISAGED IN THE METHODS OF BALANCE EXPERIMENTS

    Directory of Open Access Journals (Sweden)

    Petar Batsalov

    2006-12-01

    Full Text Available The biochemical indexes “glycogen in liver” and “ketones in blood” of 0-72 hours feed deprived (according to methods for balanced experiments Japanese quails with and without energy additives were determined. There were 2 groups of birds- 1-st without energy supplement, 2-nd- fed with 1 g. glucose per os (as 25% solution – twice in 24 hours. The levels of liver glycogen in all the food-deprived quails were signifi cantly lower from -6910 (12-th hour of starving-to 4960mg/kg (72 hour of starving compared to the levels of the same index in fed birds (11990 mg/kg tissue. In the birds receiving energy additive they were higher compared to those deprived of the additive throughout the experimental period. The content of ketones in blood of the control birds was 0.015 mmol/l. The same index increased to 0.027 mmol/l in the feed and energy additive deprived group after the 36 hour of starving, but in the group became energy support, the contents of ketones were lower for the whole period of starving. The energy additive (1g glucose/24 hours helped the maintenance of the energy metabolism during continuous food depriving of the experimental quails.

  17. A Method for Fast Assessment of OP/CB Exposure in the Japanese Quail (Coturnix coturnix japonica Using Combined Esterases Enzyme Activity as Biomarkers

    Directory of Open Access Journals (Sweden)

    Kasim Sakran Abass

    2014-01-01

    Full Text Available The aims of this study were to investigate the presence of different esterase activities in plasma and liver for Japanese quail and to combine determination of both carboxylesterase and cholinesterase as biochemical biomarker in order to identify the effects of carbamate and organophosphate compounds exposure. Carboxylesterase exhibits larger sensitivity to carbamate and organophosphate compounds than to cholinesterase and is present at higher levels. This permitted nature and distribution of carboxylesterase or cholinesterase to be measured. One predominant toxicological form of enzyme level constant in its patterns of motivation and inhibition with cholinesterase was identified in plasma with an apparent Michaelis constant for butyrylthiocholine iodide of 0.394 mM. Carboxylesterase activity in liver was considered by its preferential hydrolysis of the S-phenyl thioacetate. A concentration dependent decrease of carboxylesterase and cholinesterase has demonstrated during in vitro incubation of malathion, parathion, and trichlorfon in the range 0.125–2 mM, while with methomyl was in the range 0.25–4 mM. When quail (n=15 was exposed orally for 48 h to concentrations of carbamate or organophosphate compounds of 3–200 mg/kg, the percentage inhibition of cholinesterase was in each case larger than that of carboxylesterase and reached statistical significance (P<0.05 at lower concentrations.

  18. Milheto (Pennisetum glaucum (L. R.Br. como substituto do milho em rações para codornas-japonesas em postura (Coturnix coturnix japonica

    Directory of Open Access Journals (Sweden)

    Leandro N.S.M.

    1999-01-01

    Full Text Available Avaliou-se o desempenho de codornas-japonesas em postura alimentadas com níveis crescentes de milheto-grão na ração. Duzentas codornas com 70 dias de idade, submetidas a um delineamento inteiramente ao acaso, com cinco tratamentos e quatro repetições de 10 aves, receberam níveis de milheto de 0, 25, 50, 75 e 100% em substituição ao milho em rações na fase de postura. Foram medidos a produção e o peso dos ovos, o consumo de ração, a mortalidade, a coloração da gema pelo leque colorimétrico, e calculada a conversão alimentar em termos de massa e de dúzia de ovos. Os índices de produtividade das codornas não foram afetados pelos diferentes níveis de substituição estudados e o milheto-grão se constituiu em uma fonte de substituição do milho para codornas-japonesas, devendo-se, porém, incluir na ração uma fonte de pigmentação para a gema dos ovos.

  19. DDE increased the toxicity of parathion to coturnix quail

    Science.gov (United States)

    Ludke, J.L.

    1977-01-01

    Adult male Japanese quail (Coturnix coturnix) were exposed to DDE or chlordane in the diet and subsequently dosed with parathion or paraoxon. Pretreatment with 5 or 50 ppm DDE in the diet for 12 weeks resulted in increased cholinesterase (ChE) activity in plasma, but not in the brain. Dietary concentrations of 5 and 50 ppm DDE caused increased susceptibility of quail that were challenged with parathion or paraoxon. The increased mortality resulting from DDE pretreatment was reflected in brain ChE inhibition. The synergistic action of DDE was apparent after 3 days of exposure to 50 ppm DDE and 1 week of exposure to 5 ppm DDE. Birds exposed for 3 weeks to 5 or 50 ppm DDE retained their DDE-potentiated sensitivity to parathion after 2 weeks on clean diet. Chlordane pretreatment resulted in decreased susceptibility (antagonism) to parathion, but not to paraoxon dosage. Implications of differing responses in ChE and mortality among controls, DDE-, and chlordane-pretreated birds after parathion or paraoxon dosage are discussed.

  20. Effect of long-term selection for egg production on eggshell quality of Japanese quail (Coturnix japonica).

    Science.gov (United States)

    Fathi, M M; El-Dlebshany, A E; El-Deen, M Bahie; Radwan, L M; Rayan, G N

    2016-11-01

    An experiment was conducted to evaluate egg quality and ultrastuctural measurements of eggshell using a Scanning Electron Microscope (SEM) in 2 lines (selected and control) of Japanese quail. A selection program was applied over 22 consecutive generations for higher egg production and lower broken egg percentage. The results revealed that the females of the selected line produced significantly (P < 0.01) higher egg mass compared to that of the control line. Also, the selection procedure significantly improved feed conversion ratio. The eggshells of the selected line had a higher breaking strength compared to those of the control line, although there was no difference between them in shell thickness. Significantly higher wet (P < 0.01) and dry (P < 0.05) eggshell percentages were found in the selected line. In general, the eggshells of the selected line had a lower total score (good) of ultrastructural evaluation compared to the control line. According to scanning electron microscope data, the incidence of certain structural variants was more common in eggshells of the control line suggesting poor shell strength. The incidence of alignment was more prevalent in control eggshells compared to selected ones, suggesting lower resistance to breakage. Late fusion and large interstitial spaces of the palisade layer indicating decreased resistance to fracture were observed in control eggshells. It could be concluded that the improvement eggshell quality may be caused by the long-term selection for lower cracked and broken egg rates from generation to generation. © 2016 Poultry Science Association Inc.

  1. Behavioral and neuropharmacological evidence that serotonin crosses the blood-brain barrier in Coturnix japonica (Galliformes; Aves

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    PA. Polo

    Full Text Available This study was carried out aiming to reach behavioral and neuropharmacological evidence of the permeability of the blood-brain barrier (BBB to serotonin systemically administered in quails. Serotonin injected by a parenteral route (250-1000 µg.kg-1, sc elicited a sequence of behavioral events concerned with a sleeping-like state. Sleeping-like behaviors began with feather bristling, rapid oral movements, blinking and finally crouching and closure of the eyes. Previous administration of 5-HT2C antagonist, LY53857 (3 mg.kg-1, sc reduced the episodes of feather bristling and rapid oral movements significantly but without altering the frequency of blinking and closure of the eyes. Treatment with the 5-HT2A/2C antagonist, ketanserin (3 mg.kg-1, sc did not affect any of the responses evoked by the serotonin. Quipazine (5 mg.kg-1, sc a 5-HT2A/2C/3 agonist induced intense hypomotility, long periods of yawning-like and sleeping-like states. Previous ketanserin suppressed gaping responses and reduced hypomotility, rapid oral movements and bristling but was ineffective for remaining responses induced by quipazine. Results showed that unlike mammals, serotonin permeates the BBB and activates hypnogenic mechanisms in quails. Studies using serotoninergic agonist and antagonists have disclosed that among the actions of the serotonin, feather bristling, rapid oral movements and yawning-like state originated from activation of 5-HT2 receptors while blinking and closure of the eyes possibly require other subtypes of receptors.

  2. Appraisal and standardization of curvilinear velocity (VCL) cut-off values for CASA analysis of Japanese quail (Coturnix japonica) sperm.

    Science.gov (United States)

    Farooq, U; Malecki, I A; Mahmood, M; Martin, G B

    2017-06-01

    One of the basic steps in objective analysis of sperm motility is the subdivision of a motile sperm population into slow, medium and rapid categories based on their velocity. However, for CASA analysis of quail sperm, the velocity values for categorization of slow, medium and rapid sperm have not yet been standardized. To identify the cut-off values of "velocity curvilinear" (VCL) for quail sperm categorization, we captured and analysed 22,300 tracks of quail sperm using SCA ® -CASA. The median and mean VCL values were 85 and 97 μm/s. To define the VCL cut-off values, we used two methods. In the first, we identified the upper (rapid sperm) and lower (slow sperm) cut-off values using: (i) median VCL ± 25% or ± 50% or ± 75% of median VCL value; (ii) first and third quartile values of VCL data (i.e. 25% cut-off setting); and (iii) 33% and 66% of VCL data. Among these settings, sperm categories and their corresponding motility characteristics recorded using the "25%" setting (i.e. slow ≤36 ≤ medium ≤154 ≤ rapid) were found the most realistic and coherent with male ranking by fertility. In the second method, we calculated heteroscedasticity in the total VCL data using PCA and the two-step clustering method. With this approach, the mean of the high and low clusters was 165 and 51 μm/s, respectively. Together, the mean from two methods suggested that, for SCA ® -CASA categorization of quail sperm, sperm should be classed as "rapid" at VCL ≥160 μm/s and "slow" at VCL ≤45 μm/s. © 2017 Blackwell Verlag GmbH.

  3. Túnel bajo el agua

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    Stamhuis, E.

    1964-03-01

    Full Text Available This article describes the results obtained by the Dutch Central Institute of Scientific Research when studying the problem of underwater tunnels. This Institute has sought to establish general specifications for the design of this type of tunnels, so that they shall be more efficient than those at present in use. Firstly, a study was made of the strength, waterproof qualities, and most rational type of ventilation, with a view to drawing up of general principles on this aspect of the design. An analysis is given of the shape, suitable cross sections and constructive methods, and these are compared with those adopted in the construction of underwater tunnels at present in use in America. Particular attention has been given to designs which are largely based on prefabrication and the use of factory made structural parts, and also on the transport and erection of these at the site. The author goes on to discuss the interplay of soil and water pressures, buoyancy forces, and cases in which the tunnel is subjected to important bending and shear forces on particular sections of it. In this connection care is taken to give the theoretical considerations a ready practical interpretation. In particular, a description is given of special distance pieces which connect the two tubes making up a tunnel, also the system of joining two sections of the tube, and the measure of watertightness and flexibility which such joints provide. Finally, a summary is given of the theoretical foundations, as well as a number of observations and items of advise, which are applicable to these types of structures.El ingeniero A. Eggink describió, en un trabajo publicado últimamente, un nuevo tipo de túnel bajo el agua para canalizar la circulación rodada. En este trabajo se desarrolla un análisis sobre las consideraciones que han jugado un papel predominante en la redacción del proyecto de ejecución de un túnel subacuo. Acompañan al trabajo los resultados referentes

  4. Eficácia de Chenopodium ambrosioides (erva-de-santa-maria no controle de endoparasitos de Coturnix japônica (codorna japonesa

    Directory of Open Access Journals (Sweden)

    Gilmar F. Vita

    2015-05-01

    Full Text Available Resumo:O objetivo foi testar in vitro e in vivo a eficácia da planta medicinal Chenopodium ambrosioidesLinnaeus, 1786 (erva-de-santa-maria, nas formas fitoterápica e homeopática, como meios alternativos para o controle de endoparasitos de Coturnix japonica Temminck & Schlegel, 1849 (codorna japonesa, um sério problema que afeta a criação e desempenho de aves domésticas, ocasionando morte quando muito intenso, retardo de crescimento, redução de índice de conversão alimentar e aumento na suscetibilidade às doenças infecciosas. As metodologias utilizadas foram preconizadas por Coles et al. (1992, creditada pela World Association for the Advancement of Veterinary Parasitology (WAAVP. A pesquisa evidenciou a presença dos gêneros Ascaridiae Eimeria. O ensaio in vitro demonstrou alta taxa de redução na inibição de eclosão de ovos de Ascaridiasp. (100,00% e significativa taxa de redução na destruição de oocistos de Eimeriasp. (47,06%. O ensaio in vivodemonstrou alta taxa de redução na contagem de ovos de Ascaridiasp. nas fezes (100,00% e expressiva taxa de redução na contagem de oocistos de Eimeriasp. nas fezes (60,33%. Chenopodium ambrosioides mostrou em certos momentos superioridade frente ao produto tradicional (Thiabendazole/Mebendazole e índices superiores aos preconizados pelo Ministério da Agricultura do Brasil e Organização Mundial da Saúde como indicativos de eficácia.

  5. Effects of Sophora japonica flowers (Huaihua on cerebral infarction

    Directory of Open Access Journals (Sweden)

    Hsieh Ching-Liang

    2010-09-01

    Full Text Available Abstract The dried flowers and buds of Sophora japonica are used as a medicinal herb in China, Japan and Korea to treat bleeding hemorrhoids and hematemesis. This article presents an overview of the effects of Sophora japonica on cerebral infarction based on literature searched from Medline, PubMed, Cochrane Library and the China National Knowledge Infrastructure (CNKI. Sophora japonica contains both anti-hemorrhagic and anti-hemostatic substances. Sophora japonica reduces cerebral infarction partly as a result of its anti-oxidative and anti-inflammatory activities. Previous studies found that Sophora japonica reduced the size of cerebral infarction and neurological deficits and reduced microglial activation, interleukin-1β release and number of apoptotic cells in ischemia-reperfusion injured Sprague-Dawley rats. Further study is required to determine the relationship between Sophora japonica-mediated reduction in cerebral infarction size and the effects of Sophora japonica on platelet aggregation and cardiovascular function.

  6. Viviendas de muy bajo coste

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    Lemus, V.

    1982-11-01

    Full Text Available Not available.En el Instituto de Investigaciones en Materiales de la Universidad Nacional Autónoma de México, se está desarrollando un área de investigación sobre tecnología de materiales y tecnología de sistemas constructivos, la cual está integrada por diversos proyectos encaminados a solucionar problemas relativos a la vivienda. El presente trabajo consiste en el desarrollo de materiales compuestos a base de desechos agrícolas e industriales, con características tales como baja densidad, resistencia adecuada, excelentes propiedades térmicas y acústicas y, fundamentalmente, bajo costo y gran disponibilidad. Los resultados obtenidos son bastante alentadores debido a la durabilidad y resistencia mecánica obtenidos a base del tratamiento proporcionado a la materia orgánica. Se comentan las aplicaciones actuales y futuras de estos nuevos materiales.

  7. Assessment of long-range correlation in animal behavior time series: The temporal pattern of locomotor activity of Japanese quail (Coturnix coturnix) and mosquito larva (Culex quinquefasciatus)

    Science.gov (United States)

    Kembro, Jackelyn M.; Flesia, Ana Georgina; Gleiser, Raquel M.; Perillo, María A.; Marin, Raul H.

    2013-12-01

    Detrended Fluctuation Analysis (DFA) is a method that has been frequently used to determine the presence of long-range correlations in human and animal behaviors. However, according to previous authors using statistical model systems, in order to correctly use DFA different aspects should be taken into account such as: (1) the establishment by hypothesis testing of the absence of short term correlation, (2) an accurate estimation of a straight line in the log-log plot of the fluctuation function, (3) the elimination of artificial crossovers in the fluctuation function, and (4) the length of the time series. Taking into consideration these factors, herein we evaluated the presence of long-range correlation in the temporal pattern of locomotor activity of Japanese quail (Coturnix coturnix) and mosquito larva (Culex quinquefasciatus). In our study, modeling the data with the general autoregressive integrated moving average (ARFIMA) model, we rejected the hypothesis of short-range correlations (d=0) in all cases. We also observed that DFA was able to distinguish between the artificial crossover observed in the temporal pattern of locomotion of Japanese quail and the crossovers in the correlation behavior observed in mosquito larvae locomotion. Although the test duration can slightly influence the parameter estimation, no qualitative differences were observed between different test durations.

  8. con bajo peso al nacer

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    Adriana Mora Antó

    2005-01-01

    Full Text Available Esta investigación dio cuenta de la relación entre el estilo de funcionamiento familiar, los patrones de crianza y las edades de desarrollo evolutivo en niños, nacidos con bajo peso. El estudio descriptivo correlacional se realizó con 41 niños y sus madres, aplicándose cuestionarios sobre funcionamiento familiar, prácticas de crianza y desarrollo infantil. Los resultados señalaron la existencia de un funcionamiento familiar caracterizado por una cohesión amalgamada y una adaptabilidad caótica, una disciplina complaciente, falta de control y de límites claros en la díada madre-hijo. Se trataba de familias monoparentales, donde la temprana edad de concepción, el madresolterismo y el apoyo de la familia extensa eran constantes. Las edades evolutivas registradas indicaron un desarrollo inferior a la edad cronológica, en la mayor parte de los casos; sin embargo, éstas tendieron a ser superiores al compararlas con la edades reales de los infantes. No se encontró una correlación estadísticamente significativa entre la edad de desarrollo y los diferentes factores del funcionamiento familiar para algunos de los rangos de edad considerados; sin embargo, no se lo descartó por completo, especialmente en lo referente al optimismo familiar

  9. IDENTIFICATION OF WIDE COMPATIBILITY VARIETIES IN SOME TROPICAL JAPONICA RICE

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    A. Hairmansis

    2013-07-01

    Full Text Available The F1 hybrid sterility in indica/japonica crosses is the major barrier in developing hybrid rice varieties between these two diverse germplasm. The sterility problem in japonica/indica hybrids can be overcome by using wide compatibility genes. The objective of this study was to identify wide compatibility varieties (WCVs in some tropical japonica rice. Twenty five tropical japonica varieties as male parents were crossed with indica (IR64 and japonica (Akitakomachi testers as female parents. The crosses were planted following a randomized complete block design with three replications. Varieties having average spikelet fertility of more than 70% with both the indica and japonica testers were rated as WCVs. Result from this study showed that six tropical japonica varieties were classified as WCVs, i.e., Cabacu, Grogol, Kencana Bali, Klemas, Lampung Lawer, and Napa. Hybrid sterility is caused by partial sterility of male and female gametes. The WCVs from the present study can be used in hybrid rice breeding program to solve hybrid sterility in indica/japonica hybrids.

  10. One Generation Reproductive Toxicity Test in Japanese quail (Coturnix japonica) Exposed Orally to 3-nitro-1,2,4-triazol-5-one (NTO), February-August 2015

    Science.gov (United States)

    2017-08-14

    opisthotonos), and alternated between prostrate inactivity and ataxic wing activity. In conjunction with neuromuscular signs, decreased body mass...prostrate inactivity and ataxic wing activity beginning 3-4 hours after dosing. In conjunction with neuromuscular signs, decreased body mass gain...of the most concentrated suspension. In conjunction with a previous study, samples were collected from a representative suspension (6 mg/ml) at

  11. Response surface methodology based optimization for degradation of align in Laminaria japonica feedstuff via fermentation by Bacillus in Apostichopus japonicas farming

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    Xitao Wang

    2016-07-01

    Conclusions: This study provides an alternative and economical way to reduce the algin content in L. japonica through degradation by WB1, making it a promising potential source of feed for cultured L. japonica.

  12. Morphological and genetic differences between Coptis japonica var. anemonifolia H. Ohba and Coptis japonica var. major Satake in Hokuriku area.

    Science.gov (United States)

    Kitamura, Masashi; Ando, Hirokazu; Sasaki, Yohei

    2018-03-01

    Coptis japonica is widely distributed in Japan, and its dried rhizome is a source of the domestic herbal medicine Coptidis Rhizoma ( Oren). There are three varieties of C. japonica, two of which, namely, C. japonica var. anemonifolia and C. japonica var. major, are important as sources of traditional medicines. Coptis japonica var. anemonifolia and C. japonica var. major are distinguishable on the basis of their ternate or biternate compound leaves, respectively. In the Hokuriku area, where both C. japonica var. anemonifolia and C. japonica var. major grow naturally, some individual plants cannot be identified unambiguously on the basis of leaf morphology because changes in leaf morphology may occur due to intra-variety variation or crossbreeding between the two varieties. In addition, genetic differences between the two varieties have remained unclear. In this study, we employed new genetic and morphological classification approaches to discriminate between the two varieties. Based on the single nucleotide polymorphisms of the tetrahydroberberine oxidase gene, we found four conserved SNPs between the two varieties and were able to classify C. japonica into two varieties and crossbreeds. Furthermore, we introduced a new leaf type index based on the overall degree of leaflet dissection calculated by surface area of a leaflet and length of leaflet margin and petiolule. Using our new index we were able to discriminate between the two varieties and their crossbreeds more accurately than is possible with the conventional discrimination method. Our genetic and morphological classification methods may be used as novel benchmarks to discriminate between the two varieties and their crossbreeds.

  13. The intangible legacy of the Indonesian Bajo

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    Chandra Nuraini

    2016-05-01

    Full Text Available The Sama-Bajau, or Bajo diaspora, extends from the southern Philippines and Sabah (Malaysian Borneo to the eastern part of Indonesia. The Indonesian Bajo, now scattered along the coasts of Sulawesi (Celebes and East Kalimantan, the Eastern Lesser Sunda Islands and Maluku, were once mostly nomadic fishermen of the sea or ocean freight carriers. Today, the Bajo are almost all fishermen and settled. Their former and present ways of life made them favour intangible forms of culture: it is impossible to transport bulky artefacts when moving frequently by boat, or when living in stilt houses, very close to the sea or on a reef. It is therefore an intangible legacy that is the essence of the Bajo’s culture. Sandro healers have a vast range of expertise that allows them to protect and heal people when they suffer from natural or supernatural diseases. On the other hand, music and especially oral literature are very rich. In addition to song and the pantun poetry contests, the most prestigious genre is the iko-iko, long epic songs that the Bajo consider to be historical rather than fictional narratives. The Bajo’s intangible heritage is fragile, since it is based on oral transmission. In this article, I give a description of this heritage, dividing it into two areas: the knowledge that allows them to “protect and heal” on the one hand, and to “distract and relax”, on the other.

  14. The Influence of Different Processing Methods on Component Content of Sophora japonica

    Science.gov (United States)

    Ji, Y. B.; Zhu, H. J.; Xin, G. S.; Wei, C.

    2017-12-01

    The purpose of this experiment is to understand the effect of different processing methods on the content of active ingredients in Sophora japonica, and to determine the content of rutin and quercetin in Sophora japonica under different processing methods by UV spectrophotometry of the content determination. So as to compare the effect of different processing methods on the active ingredient content of Sophora japonica. Experiments can be seen in the rutin content: Fried Sophora japonica>Vinegar sunburn Sophora> Health products Sophora japonica> Charred sophora flower, Vinegar sunburn Sophora and Fried Sophora japonica difference is not obvious; Quercetin content: Charred sophora flower> Fried Sophora japonica> Vinegar sunburn Sophora>Health products Sophora japonica. It is proved that there are some differences in the content of active ingredients in Sophora japonica in different processing methods. The content of rutin increased with the increase of the processing temperature, but the content decreased after a certain temperature; Quercetin content will increase gradually with time.

  15. Japonica rice variety Yangfujing 7 bred by mutation

    International Nuclear Information System (INIS)

    He Zhentian; Chen Xiulan; Han Yuepeng; Wang Jinrong; Yang Hefeng; Wang Jianhua

    2006-01-01

    A japonica variety, Yangfujing 7, which possessing high yield potential, good quality and disease resistance was developed through cross breeding combined mutation breeding. The variety met the needs of agricultural environment in Jiangsu province. (authors)

  16. Different Aluminum Tolerance among Indica, Japonica and Hybrid Rice Varieties

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    Shu Chang

    2015-05-01

    Full Text Available Hydroponic cultures were conducted to compare the aluminum (Al tolerance among different rice (Oryza sativa L. varieties, including indica, japonica and their hybrids. The results showed that the root growth of rice plant was inhibited in different degrees among Al treated varieties. The Al tolerance observed through relative root elongation indicated that five japonica varieties including Longjing 9, Dharial, LGC 1, Ribenyou and Koshihikari were relatively more tolerant than indica varieties. Most indica varieties in this study, such as Aus 373 and 9311 (awnless, were sensitive to Al toxicity. The Al tolerance of most progenies from japonica × indica or indica × japonica crosses was constantly consistent with indica parents. The differences of Al tolerance among Longjing 9 (japonica, Yangdao 6 (indica and Wuyunjing 7 (japonica were studied. Biomass and the malondial-dehyde content of Yangdao 6 under Al exposure decreased and increased, respectively, while there was no significant effect on those of Longjing 9 and Wuyunjing 7. Remarkable reduction of root activities was observed in all these three rice varieties. Significantly higher Al content in roots was found in Yangdao 6 compared to Longjing 9 or Wuyunjing 7.

  17. [Two kaempferol triglycosides from pericarps of Sophora japonica L].

    Science.gov (United States)

    Tang, Y P; Lou, F C; Wang, J H

    2001-12-01

    To study the flavonol triglycosides in the pericarps of Sophora japonica. Various chromatographic techniques were used to isolate and purify the constituents. The structures were elucidated by chemical evidence and spectral analysis, especially by 2D NMR experiments. Two kaempferol triglycosides were isolated and identified as kaempferol 3-O-beta-D-sophoroside-7-O-alpha-L-rhamnoside and kaempferol 3-O-(2"-O-beta-D-glucosyl)-beta-D-rutinoside. Both of them were reported in S. japonica for the first time.

  18. Anti-inflammatory and antioxidant properties of Eriobotrya japonica ...

    African Journals Online (AJOL)

    Abstract. Background: In the present work we determined phenolic and flavonoids content of Eriobotrya japonica leaves extracts and fractions and their antioxidant and anti-inflammatory properties. Objectives: To evaluate the inhibition of inflammatory PLA2 and antioxidant effects of extracts and fractions from Erio-.

  19. The naturalization of "loquat", Eriobotrya japonica (Rosaceae, Maloideae in Argentina

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    Gustavo Deluchi

    2010-01-01

    Full Text Available Based on herbarium specimens collected in our country, Eriobotrya japonica(Thunb. Lindl. (Rosaceae is mentioned for the first time as naturalized plant in Argentina. Ecological observations and information about various local uses are included. The species is described and illustrated.

  20. Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum.

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    Jin Cai

    Full Text Available Bacterial ring rot of potato is one of the most serious potato plant and tuber diseases. Laminaria japonica extract was investigated for its antimicrobial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff Davis et al., the causative agent of bacterial ring rot of potato. The results showed that the optimum extraction conditions of antimicrobial substances from L. japonica were an extraction temperature of 80°C, an extraction time of 12 h, and a solid to liquid ratio of 1∶25. Active compounds of L. japonica were isolated by solvent partition, thin layer chromatography (TLC and column chromatography. All nineteen fractionations had antimicrobial activities against C. michiganense subsp. sepedonicum, while Fractionation three (Fr.3 had the highest (P<0.05 antimicrobial activity. Chemical composition analysis identified a total of 26 components in Fr.3. The main constituents of Fr.3 were alkanes (80.97%, esters (5.24%, acids (4.87% and alcohols (2.21%. Antimicrobial activity of Fr.3 against C. michiganense subsp. sepedonicum could be attributed to its ability to damage the cell wall and cell membrane, induce the production of reactive oxygen species (ROS, increase cytosolic Ca2+ concentration, inhibit the glycolytic pathway (EMP and tricarboxylic acid (TCA cycle, inhibit protein and nucleic acid synthesis, and disrupt the normal cycle of DNA replication. These findings indicate that L. japonica extracts have potential for inhibiting C. michiganense subsp. sepedonicum.

  1. [Gene cloning and bioinformatics analysis of SABATH methyltransferase in Lonicera japonica var. chinensis].

    Science.gov (United States)

    Yu, Xiao-Dan; Jiang, Chao; Huang, Lu-Qi; Qin, Shuang-Shuang; Zeng, Xiang-Mei; Chen, Ping; Yuan, Yuan

    2013-08-01

    To clone SABATH methyltransferase (rLjSABATHMT) gene in Lonicera japonica var. chinensis, and compare the gene expression and intron sequence of SABATH methyltransferase orthologous in L. japonica with L. japonica var. chinensis. It provide a basis for gene regulate the formation of L. japonica floral scents. The cDNA and genome sequences of LjSABATHMT from L. japonica var. chinensis were cloned according to the gene fragments in cDNA library. The LjSABATHMT protein was characterized by bioinformatics analysis. SABATH family phylogenetic tree were built by MEGA 5.0. The transcripted level of SABATHMT orthologous were analyzed in different organs and different flower periods of L. japonica and L. japonica var. chinensis using RT-PCR analysis. Intron sequences of SABATHMT orthologous were also analyzied. The cDNA of LjSABATHMT was 1 251 bp, had a complete coding frame with 365 amino acids. The protein had the conservative SABATHMT domain, and phylogenetic tree showed that it may be a salicylic acid/benzoic acid methyltransferase. Higher expression of SABATH methyltransferase orthologous was found in flower. The intron sequence of L. japonica and L. japonica var. chinensis had rich polymorphism, and two SNP are unique genotype of L. japonica var. chinensis. The motif elements in two orthologous genes were significant differences. The intron difference of SABATH methyltransferase orthologous could be inducing to difference of gene expression between L. japonica and L. japonica var. chinensis. These results will provide important base on regulating active compounds of L. japonica.

  2. cultivadas bajo un gradiente de sombra

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    Marco V. Gutiérrez

    2007-01-01

    Full Text Available Se evaluó el crecimiento de 9 especies de palmas cultivadas bajo un gradiente de sombra producido por mallas de polipropileno negro de 40, 50, 60, 70 y 80% de sombra, más un tratamiento de malla aluminizada de 70%, y uno con plantas a pleno sol. Las especies evaluadas fueron Caryota mitis (cola de pez, Chamaedorea costaricana (pacaya, Chamaedorea tepejilote (tepejilote, Dypsis lutescens (areca, Licuala elegans (licuala, Phoenix roebelenii (fénix, Ptychosperma macarthurii (palma macarthur, Roystonea regia (palma real, y Veitchia merrillii (navideña. Se midió la altura de las plantas, la longitud de las hojas maduras, y el número de hojas cosechadas, durante 5 cosechas por 2 años. En general, la altura de las plantas y la longitud de las hojas fueron menores a 0-40% de sombra, se incrementaron a 50-70%, y decrecieron a 80%. C. mitis, C. tepejilote, L. elegans y P. macarthurii, se comportaron como especies obligadas de sombra y no sobrevivieron a pleno sol. C. costaricana y D. lutescens sobrevivieron a plena exposición solar, y su crecimiento alcanzó valores máximos a 50-60%. P. roebelenii, R. regia, y V. merrillii mostraron un crecimiento reducido a 0-40%, pero éste mejoró bajo los demás niveles de sombra a lo largo del gradiente. En general, 1-2 años es un periodo apropiado para el cultivo de palmas de crecimiento rápido (R. regia, Chamedorea spp., D. lutescens en casas de mallas. Palmas de lento crecimiento (L.elegans pueden permanecer 3-5 años en una casa de sombra. Se discute estrategias para el uso de gradientes de sombra en el tiempo y en el espacio, según la utilidad y los requerimientos de las especies, los requisitos establecidos por el mercado, y el ciclo de producción del material vegetal.

  3. Studies on Schistosomiasis japonica and Saponins

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    Edito Garcia

    1980-01-01

    Full Text Available The molluscicidal activity of the bark of Entada phaseoloides and extracts from tubers of its related specie, Entada parvifolia against Oncomelania quadrasi, the snail intermediate host of Shistosoma japonicum in the Philippines were determined.The commercial gogo bark applied to waters with O. quadrasi, in the proportion of 2 gms/liter will kill 100% of snails within 24 hours. At this concentration miracidia and cercariae of S. japonicum in the same waters will die within one hour, thus, making the water safe or noninfective for some time. At the dose 100 gms/sqm of water-covered terrestial snail habitats, provided previously cleared of vegetations, at least 90% of O. quadrasi will die within 24 hours. These measures can be practical on a self-help basis by inhabitants of schistosomiasis endemic areas where gogo plant grows or where its bark is marketed.At dilution of 1:5,000 saponin extracted from tubers of E. parvifolia killed at least 90% of snails after 24 hours exposure while ethanol (crude saponin and other extracts require at least 1:2,000 concentration to kill at least 90% of O. quadrasi. At these concentrations and the expenses and time involved in the preparation of these extracts, they are not economical or practical for large scale use of molluscicides.It is suggested that methods of extraction and purification which require little time of preparation and a cheaper but of higher recovery rate of molluscicidal principles be developed.Surveys of barrios in three towns of Leyte, endemic for Schistosomiasis japonica using the circum oval-precipitin test (COPT and stool examination in the same subjects were undertaken. Findings show that the use of blood COPT method is advantageous over that of the stool examination in schistosomiasis surveys as its prescribed procedure is simple, specific and more sensitive. Moreover, the prevalence rate it determined was higher than that obtained by the stool examination in all three towns studied with

  4. Viviendas de bajo costo sistema SALVY

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    Suárez Ibarra, Salvador

    1984-06-01

    Full Text Available The aim of this work is to offer an alternative for solving the current problem of housing, through a building system which has been developed and tested in our country — the SAL VY SYSTEM. It is directed towards solving the problem of low-cost housing with own technology, enabling these houses to be built quickly, using materials which are not traditional in building, but are abundant in our country. The house presented by us allows the user to extend its height according to his needs and financial possibilities, keeping the free area of the plot for so-called kitchen gardens.Este trabajo tiene como objetivo presentar una alternativa para la solución del problema actual de la vivienda, a través de un sistema constructivo desarrollado y experimentado en nuestro país —SISTEMA SALVY, dirigido a solucionar el problema de la vivienda de bajo costo, con una tecnología propia que permite la construcción de estas viviendas de una manera rápida, utilizando materiales no tradicionales en construcción, pero abundantes en nuestro país. La vivienda que presentamos permite al usuario ampliarla en altura, de acuerdo a sus necesidades y posibilidades económicas, manteniendo el área libre de la parcela para los denominados huertos del hogar.

  5. Effects of storage and gamma irradiation on (japonica) waxy rice

    International Nuclear Information System (INIS)

    Sung, W.-C.; Hong, Mei-Chu; Chang, T.-S.

    2008-01-01

    Japonica cultivar, Taichung waxy 70 (TCW 70), was exposed to gamma radiation with doses ranging from 0 to 2.0 kGy. The effects of gamma irradiation on waxy rice pasting properties and the qualities of its food product, mochi, were compared to the effects of storage over 12 months. Doses ranging from 0.5 to 2 kGy can decrease the paste viscosity of waxy rice as those obtained after 6 months of storage. Radiation treatments were less effective to decrease the hardness of mochi than waxy rice samples stored over 6 months. It was shown the effects of gamma irradiation on shortening the japonica waxy rice aging time and improving the quality of rice products, like mochi, were not as good as the effects of storage

  6. Effects of storage and gamma irradiation on (japonica) waxy rice

    Energy Technology Data Exchange (ETDEWEB)

    Sung, W.-C. [Department of Hotel and Restaurant Management, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Sec. 1, Pao-An, Jen-Te Hsiang, Tainan 717, Taiwan (China)], E-mail: sungwilliam2001@yahoo.com.tw; Hong, Mei-Chu [TaiChung District Agricultural Research and Extension Station Council of Agriculture Executive Yuan, Taichung, Taiwan (China); Chang, T.-S. [Department of Biological Sciences and Technology, National University of Tainan, Tainan, Taiwan (China)

    2008-01-15

    Japonica cultivar, Taichung waxy 70 (TCW 70), was exposed to gamma radiation with doses ranging from 0 to 2.0 kGy. The effects of gamma irradiation on waxy rice pasting properties and the qualities of its food product, mochi, were compared to the effects of storage over 12 months. Doses ranging from 0.5 to 2 kGy can decrease the paste viscosity of waxy rice as those obtained after 6 months of storage. Radiation treatments were less effective to decrease the hardness of mochi than waxy rice samples stored over 6 months. It was shown the effects of gamma irradiation on shortening the japonica waxy rice aging time and improving the quality of rice products, like mochi, were not as good as the effects of storage.

  7. Flavonoids and fatty acids of Camellia japonica leaves extract

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    Cinthia M. Azuma

    2011-07-01

    Full Text Available The ethanol extract from the leaves of Camellia japonica L., Theaceae, showed antiradical potential in the DPPH test using TLC plates (SiO2. Aiming the isolation of active compounds, this extract was partitioned between BuOH:H2O (1:1 and the two obtained phases were also evaluated to detection of antiradical activity. The active BuOH phase was fractionated in Sephadex LH-20 and silica (normal or reverse phase to afford three aglycone flavonoids (quercetin, kaempferol and apigenin, which have been described in the C. japonica at first time, two glycosilated flavonoids (rutin and quercetrin, and a mixture of saturated fatty acids. The structures of isolated compounds were defined by NMR and GC/MS analyses.

  8. Flavonoids and fatty acids of Camellia japonica leaves extract

    Directory of Open Access Journals (Sweden)

    Cinthia M. Azuma

    2011-12-01

    Full Text Available The ethanol extract from the leaves of Camellia japonica L., Theaceae, showed antiradical potential in the DPPH test using TLC plates (SiO2. Aiming the isolation of active compounds, this extract was partitioned between BuOH:H2O (1:1 and the two obtained phases were also evaluated to detection of antiradical activity. The active BuOH phase was fractionated in Sephadex LH-20 and silica (normal or reverse phase to afford three aglycone flavonoids (quercetin, kaempferol and apigenin, which have been described in the C. japonica at first time, two glycosilated flavonoids (rutin and quercetrin, and a mixture of saturated fatty acids. The structures of isolated compounds were defined by NMR and GC/MS analyses.

  9. Characteristics and Application Analysis of Traditional Chinese Medicine Containing Sophora Japonica

    Science.gov (United States)

    Wei, Zhenzhen; Feng, Suxiang; Fang, Xiaoyan; Miao, Mingsan

    2018-01-01

    Purposes: To sum up the characteristics of Chinese medicine with Sophora Japonica and provide reference for the research, development and utilization of the Chinese medicine of Sophora japonica in the future. Methods: The author sums up the forms, functions, indications, usage, dosage and contraindications of the proprietary Chinese medicine containing Sophora Japonica in the Chinese Pharmacopoeia and the Ministerial standards. In addition, we will inquire about the clinical application of proprietary Chinese medicine containing Sophora japonica in the China National Knowledge Infrastructure (CNKI). Results: The proprietary Chinese medicine containing Sophora Japonica was widely used in the treatment of various diseases in clinic, but it was taken orally and without any external use of Chinese patent medicine. Moreover, in most of the proprietary Chinese medicine, Sophora japonica was used as a supplement; In addition, the causes of adverse reactions were not analyzed, and the safety of the drugs needed to be further analyzed. Conclusions: To make clear the role of Sophora japonica in proprietary Chinese medicine, we can develop the Chinese medicine new dosage forms of Sophora japonica; The Chinese medicine is made up of a variety of single herbs, some are toxic drugs, when an adverse reaction occurs, We should analyze the specific causes and avoid the occurrence of adverse reactions. In addition, Sophora japonica is a traditional herbal medicine and food in China; we can expand the application in other areas and explore the pharmacological and toxicological pathology.

  10. Genetic Differentiations among the Populations of Salvia japonica (Lamiaceae and Its Related Species

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    SUDARMONO

    2008-03-01

    Full Text Available Morphological and genetic variations within Salvia japonica (Lamiaceae and its related species in Japan were analyzed for clarifying their taxonomic significance. The genetic variations were explored through chloroplast and nuclear ribosomal DNA sequences and allozyme polymorphisms. Since chromosome numbers characterized the genus of Salvia, we also examined whether the karyotypes were different. We examined 58 populations of S. japonica and 14 populations of others species of Salvia. Among the populations of S. japonica represented four forms (f. japonica, f. longipes, f. lanuginosa and f. albiflora. The size of chromosomes were various among Salvia spp. Based on the allozyme as well as the DNA sequence, the populations of S. japonica separated from the others Salvia species. The populations of S. japonica exhibited four combinations of the morphological characters. However, these combinations did not correlate to the four forms of S. japonica. In addition, the morphological variations did not correlate to the allozyme and DNA sequences. It is suggested that the four morphological variations as well as the four form of S. japonica should not considered to be a taxonomic unit; accordingly, S. japonica were considered to be still at the early stage of speciation process.

  11. Antimicrobial Air Filters Using Natural Euscaphis japonica Nanoparticles.

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    Gi Byoung Hwang

    Full Text Available Controlling bioaerosols has become more important with increasing participation in indoor activities. Treatments using natural-product nanomaterials are a promising technique because of their relatively low toxicity compared to inorganic nanomaterials such as silver nanoparticles or carbon nanotubes. In this study, antimicrobial filters were fabricated from natural Euscaphis japonica nanoparticles, which were produced by nebulizing E. japonica extract. The coated filters were assessed in terms of pressure drop, antimicrobial activity, filtration efficiency, major chemical components, and cytotoxicity. Pressure drop and antimicrobial activity increased as a function of nanoparticle deposition time (590, 855, and 1150 µg/cm2(filter at 3-, 6-, and 9-min depositions, respectively. In filter tests, the antimicrobial efficacy was greater against Staphylococcus epidermidis than Micrococcus luteus; ~61, ~73, and ~82% of M. luteus cells were inactivated on filters that had been coated for 3, 6, and 9 min, respectively, while the corresponding values were ~78, ~88, and ~94% with S. epidermidis. Although statistically significant differences in filtration performance were not observed between samples as a function of deposition time, the average filtration efficacy was slightly higher for S. epidermidis aerosols (~97% than for M. luteus aerosols (~95%. High-performance liquid chromatography (HPLC and electrospray ionization-tandem mass spectrometry (ESI/MS analyses confirmed that the major chemical compounds in the E. japonica extract were 1(ß-O-galloyl pedunculagin, quercetin-3-O-glucuronide, and kaempferol-3-O-glucoside. In vitro cytotoxicity and disk diffusion tests showed that E. japonica nanoparticles were less toxic and exhibited stronger antimicrobial activity toward some bacterial strains than a reference soluble nickel compound, which is classified as a human carcinogen. This study provides valuable information for the development of a

  12. Túnel bajo el Elba - Hamburgo - Alemania Federal

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    Editorial, Equipo

    1976-03-01

    Full Text Available This important work, constructed to solve the traffic problem between the northern and Southern parts of Hamburg, has an overall length of 3,325 m. The greater part of the tunnel is provided with special aeration, supplied by three ventilation stations. The tunnel is made up of three independent! circulation tubes, each with two traffic lanes. The lateral tubes are intended for one way traffic, whereas the middle lane is designed for two way traffic. In the section under the river itself, these three tubes, along with the ventilating ducts, come together into one single rectangular piece. This particular stretch consists of eight members, which were constructed in nearby dry dock and were later sunk and laid on the river bottom, along a previously excavated trench. It was completed by means of highly advanced technological methods for sealing the joints and subsequently the trench was again covered up with the excavation material. The section under the greatest slope of the river bed was excavated by means of the method of the advancing driller pipe shield, and the end sections were so near the surface that the work was carried out in the open air. The northern ventilation station —a reinforced concrete construction, connecting sections of the tunnel— further contains the safety and control stations for the entire length of the tunnel, and is equipped with the most modern technical devices.Esta importante obra, construida para solucionar el problema del tráfico entre las zonas norte y sur de la ciudad de Hamburgo, tiene una longitud total de 3.325 m. En la mayor parte de su recorrido se utiliza un sistema de aireación especial, abastecido por tres estaciones de ventilación. El túnel está constituido por tres tubos de circulación independientes, con dos carriles cada uno. Los tubos laterales se utilizan para el tráfico en un solo sentido, mientras que el central permite la circulación en ambas direcciones. En el tramo bajo

  13. Factores asociados al bajo peso al nacer

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    Georgina J Peraza Roque

    2001-10-01

    Full Text Available El bajo peso al nacer (BPN ha constituido un enigma en la ciencia a través de los tiempos. Múltiples son las investigaciones realizadas acerca de las causas que lo producen y las consecuencias que provoca. Su importancia no solo radica en lo que significa en la morbilidad y la mortalidad infantil, sino que estos niños tienen habitualmente múltiples problemas posteriores. El Programa para la Reducción del BPN señala que los niños nacidos con un peso inferior a los 2 500 g presentan riesgo de mortalidad 14 veces mayor durante el primer año de vida, en comparación con los niños que nacen con un peso normal a término. Dentro de los factores de riesgo del BPN se han encontrado con mayor frecuencia en estudios realizados por diferentes autores, los siguientes: el embarazo en la adolescencia, la desnutrición en la madre, el hábito de fumar, la hipertensión arterial durante el embarazo, la sepsis cervicovaginal, la anemia y los embarazos gemelares, entre otros. Es innegable la influencia que el BPN tiene sobre las futuras generaciones, por lo que debe constituir la diana de los esfuerzos que el médico y la enfermera deben desarrollar en su prevención, sobre todo en la comunidadLow birth weight has been an enigma for Science through times. A lot of research works have been carried out about its causes and consequences. The importance of LBW not only lies in its meaning for infant mortality and morbidity but also in the many problems that low birth weight children regularly face in the future. The program for the reduction of LBW points out that newborns weighing under 2500g have during his/her first year of life a mortality risk 14 times higher than newborns with a normal weight at term. Among the low birth weight risk factors, a number of studies by various authors have frequently found the following; pregnancy at adolescence; undernourishment in mothers, smoking, blood hypertension during pregnancy, cervix-vaginal sepsis; anemia, twin

  14. Túnel bajo el puerto de Boston

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    Editorial, Equipo

    1963-05-01

    Full Text Available The new tunnel under Boston harbour has been constructed because of the insufficient capacity of a similar, earlier, tunnel to cope with the ever increasing road traffic. This tunnel is 1,550 ms long, and 9.50 ms in external diameter. There are two traffic lanes, each 3.20 ms wide. Inside it is faced with ceramic tiles, has fluorescent lighting, and ventilation is maintained by means of two ducts, one at each entrance, where the necessary compressor and other equipment has been situated. The most interesting feature of this important project is the excavation method adopted for its construction: the tunnel crosses a clay layer, which at certain places is sufficiently fluid to allow a pneumatic shield to be forced through it leaving hardly any debris behind. It will be readily appreciated that this procedure shows an obvious advantage over ordinary methods, in which it is necessary to cut out, and transport to the exterior all the excavated material. The metal sheathing was by the Commercial Shearing and Stamping Co., Youngstown, Ohio.Este nuevo túnel, bajo el puerto de Boston, es una consecuencia de la necesidad imperiosa que se hacía sentir dada la insuficiente capacidad del otro túnel similar antiguo y el aumento continuo del tráfico urbano y por carretera. Tiene 1.550 m de longitud, 9,50 m de diámetro exterior y una calzada con dos bandas de circulación de 3,20 m de anchura cada una. Está revestido ccn baldosa cerámica, iluminado con luz fluorescente, y el aire viciado se ventila y renueva por medio de dos torres de ventilación—una en cada portal—, en las que se han instalado el equipo mecánico adecuado de ventilación y compresores. La parte más interesante de esta importante obra estriba en los procedimientos empleados para llevar a cabo la excavación, pues atraviesa un banco de arcilla que, en ciertos tramos, es suficientemente fluida para permitir dejarse empujar y ceder paso a un escudo neumático que avanza sin apenas

  15. [Photosynthetic characteristics of Cuscuta japonica and its hosts during parasitization and after detachment].

    Science.gov (United States)

    Wang, Dong; Hu, Fei; Chen, Yu-Fen; Yang, Jun; Kong, Chui-Hua

    2007-08-01

    The study on the photosynthetic characteristics of Cuscuta japonica and its hosts showed that there was a negative correlation between the photosynthetic pigment content (PPC) of C. japonica and its hosts. The PPC increased in the C. japonica-preferred hosts' parasitized and neighboring leaves, but decreased in its less preferred hosts' parasitized and neighboring leaves. The leaves parasitized by C. japonica and their neighboring far from the parasitized ones had a lowered net photosynthesis rate P(n), and the decreasing order accorded with that of parasitization. The decrease of P(n) for C. japonica-less preferred hosts was mainly due to the stomatal factors, but that for the preferred hosts was regulated by multi-factors. Under light, the PPC of C. japonica detached from preferred hosts increased faster than that of C. japonica detached from less preferred hosts, but the dry matter decrease was in adverse. In dark, however, the changes in PPC and dry matter content of C. japonica were not significant, whatever hosts it was detached from.

  16. 50 CFR 226.215 - Critical habitat for the North Pacific Right Whale (Eubalaena japonica).

    Science.gov (United States)

    2010-10-01

    ... Right Whale (Eubalaena japonica). 226.215 Section 226.215 Wildlife and Fisheries NATIONAL MARINE... DESIGNATED CRITICAL HABITAT § 226.215 Critical habitat for the North Pacific Right Whale (Eubalaena japonica). (a) Primary Constituent Elements. The primary constituent elements of the North Pacific right whale...

  17. Antioxidant and Anti-Adipogenic Activities of Trapa japonica Shell Extract Cultivated in Korea

    Science.gov (United States)

    Lee, DooJin; Lee, Ok-Hwan; Choi, Geunpyo; Kim, Jong Dai

    2017-01-01

    Trapa japonica shell contains phenolic compounds such as tannins. Studies regarding the antioxidant and anti-adipogenic effects of Trapa japonica shell cultivated in Korea are still unclear. Antioxidant and anti-adipogenic activities were measured by in vitro assays such as 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity, 2,2′-azinobis( 3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, ferric reducing ability of plasma assay, reducing power, superoxide dismutase-like activity, and iron chelating ability in 3T3-L1 cells. We also measured the total phenol and flavonoids contents (TPC and TFC, respectively) in Trapa japonica shell extract. Our results show that TPC and TFC of Trapa japonica shell extract were 157.7±0.70 mg gallic acid equivalents/g and 25.0±1.95 mg quercetin equivalents/g, respectively. Trapa japonica shell extract showed strong antioxidant activities in a dose-dependent manner in DPPH and ABTS radical scavenging activities and other methods. Especially, the whole antioxidant activity test of Trapa japonica shell extract exhibited higher levels than that of butylated hydroxytoluene as a positive control. Furthermore, Trapa japonica shell extract inhibited lipid accumulation and reactive oxygen species production during the differentiation of 3T3-L1 preadipocytes. Trapa japonica shell extract possessed a significant antioxidant and anti-adipogenic property, which suggests its potential as a natural functional food ingredient. PMID:29333386

  18. Extremely Low Genomic Diversity of Rickettsia japonica Distributed in Japan.

    Science.gov (United States)

    Akter, Arzuba; Ooka, Tadasuke; Gotoh, Yasuhiro; Yamamoto, Seigo; Fujita, Hiromi; Terasoma, Fumio; Kida, Kouji; Taira, Masakatsu; Nakadouzono, Fumiko; Gokuden, Mutsuyo; Hirano, Manabu; Miyashiro, Mamoru; Inari, Kouichi; Shimazu, Yukie; Tabara, Kenji; Toyoda, Atsushi; Yoshimura, Dai; Itoh, Takehiko; Kitano, Tomokazu; Sato, Mitsuhiko P; Katsura, Keisuke; Mondal, Shakhinur Islam; Ogura, Yoshitoshi; Ando, Shuji; Hayashi, Tetsuya

    2017-01-01

    Rickettsiae are obligate intracellular bacteria that have small genomes as a result of reductive evolution. Many Rickettsia species of the spotted fever group (SFG) cause tick-borne diseases known as "spotted fevers". The life cycle of SFG rickettsiae is closely associated with that of the tick, which is generally thought to act as a bacterial vector and reservoir that maintains the bacterium through transstadial and transovarial transmission. Each SFG member is thought to have adapted to a specific tick species, thus restricting the bacterial distribution to a relatively limited geographic region. These unique features of SFG rickettsiae allow investigation of how the genomes of such biologically and ecologically specialized bacteria evolve after genome reduction and the types of population structures that are generated. Here, we performed a nationwide, high-resolution phylogenetic analysis of Rickettsia japonica, an etiological agent of Japanese spotted fever that is distributed in Japan and Korea. The comparison of complete or nearly complete sequences obtained from 31 R. japonica strains isolated from various sources in Japan over the past 30 years demonstrated an extremely low level of genomic diversity. In particular, only 34 single nucleotide polymorphisms were identified among the 27 strains of the major lineage containing all clinical isolates and tick isolates from the three tick species. Our data provide novel insights into the biology and genome evolution of R. japonica, including the possibilities of recent clonal expansion and a long generation time in nature due to the long dormant phase associated with tick life cycles. © The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  19. Identification and characterisation of ROS modulator 1 in Lampetra japonica.

    Science.gov (United States)

    Zhao, Chunhui; Feng, Bin; Cao, Ying; Xie, Peng; Xu, Jie; Pang, Yue; Liu, Xin; Li, Qingwei

    2013-08-01

    Reactive oxygen species (ROS) are a heterogeneous group of highly reactive molecules that oxidise targets in biological systems. ROS are also considered important immune regulators. In this study, we identified a homologue of reactive oxygen species modulator 1 (Romo1) in the Japanese lamprey (Lampetra japonica). The L japonica Romo1 (Lj-Romo1) gene shares high sequence homology with the Romo1 genes of jawed vertebrates. Real-time quantitative PCR demonstrated the wide distribution of Lj-Romo1 in lamprey tissues. Furthermore, after the lampreys were stimulated with lipopolysaccharide (LPS), the level of Lj-Romo1 mRNA was markedly up-regulated in the liver, gill, kidney, and intestine tissues. Lj-Romo1 was localised to the mitochondria and has the capacity to increase the ROS level in cells. The results obtained in the present study will help us to understand the roles of Romo1 in ROS production and innate immune responses in jawless vertebrates. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. Studies on chemical constituents of Polygala japonica Houtt.

    Institute of Scientific and Technical Information of China (English)

    LI Ting-zhao; ZHANG Wei-dong; LIU Run-hui; CHEN Hai-sheng; ZHANG Chuan; SU Juan; XU Xi-ke

    2006-01-01

    Objective:To investigate the chemical constituents of Polygala japonica Houtt.. Methods:The constituents were separated and purified by column chromatographies with silical gel, RP-silical gel and Sephadex LH-20. Their structures were elucidated on the basis of spectral data (IR, MS, 1H-NMR, 13C-NMR, HMQC and HMBC). Results: Totally 15 compounds were obtained from Polygala japonica Houtt. And their structures were identified as castaprenol-12 (Ⅰ), β-sitosterol (Ⅱ), stigmasterol (Ⅲ),stigmasta-7,22-di-ene-3-one (Ⅳ), n-dotriacontanol ( Ⅴ ), n-hexadecanol ( Ⅵ ), arachidic acid (Ⅶ), erogosterol (Ⅷ), kaempferol (Ⅸ), quercetin (Ⅹ), daucosterol (Ⅺ), p-hydroxybenzoic acid (Ⅻ),coumaric acid (ⅩⅢ), rhamnocitrin 3-O-β-D-galactopyranoside (ⅪⅤ), quercetin 3-O-β-D-glucopyranoside (ⅩⅤ). Conclusion: Compounds Ⅰ , Ⅲ , Ⅳ, Ⅴ , Ⅵ, Ⅶ, Ⅷ, Ⅻ , ⅩⅢ and ⅩⅣ were obtained from this plant for the first time.

  1. Transcriptome sequencing and De Novo analysis of Youngia japonica using the illumina platform.

    Directory of Open Access Journals (Sweden)

    Yulan Peng

    Full Text Available Youngia japonica, a weed species distributed worldwide, has been widely used in traditional Chinese medicine. It is an ideal plant for studying the evolution of Asteraceae plants because of its short life history and abundant source. However, little is known about its evolution and genetic diversity. In this study, de novo transcriptome sequencing was conducted for the first time for the comprehensive analysis of the genetic diversity of Y. japonica. The Y. japonica transcriptome was sequenced using Illumina paired-end sequencing technology. We produced 21,847,909 high-quality reads for Y. japonica and assembled them into contigs. A total of 51,850 unigenes were identified, among which 46,087 were annotated in the NCBI non-redundant protein database and 41,752 were annotated in the Swiss-Prot database. We mapped 9,125 unigenes onto 163 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. In addition, 3,648 simple sequence repeats (SSRs were detected. Our data provide the most comprehensive transcriptome resource currently available for Y. japonica. C4 photosynthesis unigenes were found in the biological process of Y. japonica. There were 5596 unigenes related to defense response and 1344 ungienes related to signal transduction mechanisms (10.95%. These data provide insights into the genetic diversity of Y. japonica. Numerous SSRs contributed to the development of novel markers. These data may serve as a new valuable resource for genomic studies on Youngia and, more generally, Cichoraceae.

  2. Different gene expression patterns between leaves and flowers in Lonicera japonica revealed by transcriptome analysis

    Directory of Open Access Journals (Sweden)

    Libin eZhang

    2016-05-01

    Full Text Available The perennial and evergreen twining vine, Lonicera japonica is an important herbal medicine with great economic value. However, gene expression information for flowers and leaves of L. japonica remains elusive, which greatly impedes functional genomics research on this species. In this study, transcriptome profiles from leaves and flowers of L. japonica were examined using next-generation sequencing technology. A total of 239.41 million clean reads were used for de novo assembly with Trinity software, which generated 150,523 unigenes with N50 containing 947 bp. All the unigenes were annotated using Nr, SwissProt, COGs (Clusters of Orthologous Groups, GO (Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes databases. A total of 35,327 differentially expressed genes (DEGs, P≤0.05 between leaves and flowers were detected. Among them, a total of 6,602 DEGs were assigned with important biological processes including Metabolic process, Response to stimulus, Cellular process and etc. KEGG analysis showed that three possible enzymes involved in the biosynthesis of chlorogenic acid were up-regulated in flowers. Furthermore, the TF-based regulation network in L. japonica identified three differentially expressed transcription factors between leaves and flowers, suggesting distinct regulatory roles in L. japonica. Taken together, this study has provided a global picture of differential gene expression patterns between leaves and flowers in L japonica, providing a useful genomic resource that can also be used for functional genomics research on L. japonica in the future.

  3. Behaviors of 14C-butachlor, 14C-chlorpyrifos and 14C-DDT in Rana japonica japonica Guenther

    International Nuclear Information System (INIS)

    Zhang Yiqiang; Zhong Chuangguang; Zhao Xiaokui; Chen Shunhua

    2002-01-01

    The research on the behaviors of 14 C-butachlor, 14 C-chlorpyrifos and 14 C-DDT in the frog Rana japonica japonica Guenther was carried out. After administrated per os to the frogs in doses of 380, 347, 363 Bq/g, 14 C-butachlor, 14 C-chlorpyrifos and 14 C-DDT, were distributed respectively to various organs within 24 h with specific accumulating organs as gallbladder, intestine and intestine, relevantly to the pesticides described. Compared to that in gallbladder and intestine, the radioactivity of many organs was extremely low, and this might due to the characters of the pesticides. Analysis of the metabolites of 14 C-DDT in frog at 24 th hr demonstrated that DDT was difficult to be degraded. Most 14 C-butachlor, 14 C-chlorpyrifos 14 C-DDT in liver and fat or ovary of frog was extractable with acetone. However, there were some differences between the pesticides, and the organs as well. And 14 C-butachlor, 14 C-chlorpyrifos or 14 C-DDT were better bound in liver than in fat

  4. La organización social en los barrios bajos

    Directory of Open Access Journals (Sweden)

    William Foote Whyte

    2010-07-01

    Full Text Available

    Por mucho tiempo, los sociólogos han estudiado los barrios bajos en términos de desorganización social. El interés en los mismos parece provenir de dos fuentes: una urgencia por promover reformas sociales y un esfuerzo para comprender el proceso de urbanización. Observando el quiebre de los controles del grupo primario en la vida de ciudad, algunos sociólogos han considerado analizar la situación resultante en términos del concepto de desorganización social. Los barrios bajos han sido caracterizados por ciertos sociólogos, como áreas de almas perdidas y misiones, áreas donde individuos y familias viven en intimidad forzada con personas a las cuales normalmente rechazan y evitan; áreas donde no se aprecian estándares de decencia o buena conducta excepto los impuestos por una autoridad externa. Según la opinión de otros sociólogos, los barrios bajos son un área de libertad e individualismo. Harvey Zorbaugh dice que la política en el distrito bajo no es como la política en el distrito de clase media. Como no se ajusta a los estándares de la clase media, no es más que un juego. Más aún, la preocupación con el estudio de la desorganización familiar ha conducido a algunos sociólogos a subestimar cualquier grado de organización que se pueda encontrar en los barrios bajos

  5. Harvest time of Cryptomeria japonica seeds depending on climate factors

    Science.gov (United States)

    Son, Seog-Gu; Kim, Hyo-Jeong; Kim, Chang-Soo; Byun, Kwang-Ok

    2010-05-01

    Sound seeds should have good germination rates and seed germination can be influenced by several factors. Seed picking time is regarded as one of the necessary elements to obtain sound seeds. From a clonal seed orchard of Cryptomeria japonica located in southern part of Korean peninsular, cones were picked about every 10 days from 30th of July 2005 to 30th of October in both 2005 and 2006. We have also analyzed the effects of climatic factors about two consecutive years on seed productivity. From the picked cones, seeds were collected and these germination ability, seed size and embryo shapes were investigated according to cone picking time. The 1,000-seed weight picked on 18th of August was 3.3 g and 5.3 g on 30th of September 2005and 2006. The size of seeds picked from 18th of August to 30th of September increased from 19.3 mm to 21.3 mm in length and from 15.8 mm to 18.5 mm in width. Depending on picking time, various shapes of embryos, including embryos with liquid material, jellied material and fully matured ones were observed. Germination aspects also varied throughout the test days. About two weeks after seeding in a glass petri-dish, germinal apparatuses appeared from each test seed sets which had been picked from after 10 August 2005 and 10 August 2006. The germination rates started from 10.7% from seeds picked 20 August 2006. Average germination rate in 2005 was 18.3 and 19.6 in 2006. In 2005, the highest germination rate was 34.3% from seeds picked on the 30th of September. In 2006, the highest germination rate was 31.7% for seeds picked at the same date as the 2005 seeds. After September, the highest germination rate for picked seeds decreased in both 2005 and 2006. Among the climatic factors, monthly sum of temperature and of precipitation were the main factors for maturation of C. japonica seeds. The results implied that the best cone picking time for the Korean C. japonica seed orchard to be around the end of September.

  6. RESEARCH OF SOPHORA JAPONICA L. FLOWER BUDS VOLATILE COMPOUNDS WITH GAS-CHROMATOGRAPHY/MASS- SPECTROMETRY METHOD

    Directory of Open Access Journals (Sweden)

    Cholak I.S.

    2013-10-01

    Full Text Available This work represents the results of the research ofessential oil contained in Sophora japonica L. flowerbuds volatile compounds collected during the nextstages of their development: green flower buds, formedflower buds and the beginning of flower buds opening.Essential oil assay content in Sophora japonica L.flower buds was determined with hydrodistillationmethod. Content of essential oil in the raw material isless than 0,1%. Qualitative composition and assaycontent of Sophora japonica L. flower buds essential oilconstituents were determined with chromato-massspectrometry method. In consequence of the research 80constituents were identified in Sophora japonica L.flower buds out of which 61 substances are during thegreen flower buds and beginning of flower budsopening stages, 66 substances are during formed flowerbuds stage. Substances are represented by aliphatic andcyclic terpenoids, their alcohols and ketones. Mostvolatile substances were extracted on the stage offormed buds.

  7. An annotated review of the Salamander types described in the Fauna Japonica

    NARCIS (Netherlands)

    Hoogmoed, M.S.

    1978-01-01

    The whereabouts of the salamander types described by Temminck & Schlegel in the Fauna Japonica (1838) are discussed and lectotypes are selected from the syntypes for the following nominal species : Salamandra naevia Temminck & Schlegel, S. unguiculata Temminck & Schlegel, S. subcristata Temminck &

  8. Study on the isolation of active constituents in Lonicera japonica and ...

    African Journals Online (AJOL)

    Lonicera japonica has antibacterial and gall bladder-protective effects. Objective: To study the .... Dexamethasone acetate cream (999 Pi Yan. Ping), xylene, dry yeast ... 3 ml of yeast solution. (concentration of 20%) at dorsal skin, after inducing.

  9. Characteristic features of computed tomography (CT) in hepatic schistosomiasis japonica

    Energy Technology Data Exchange (ETDEWEB)

    Sakemi, Taisuke; Sakai, Terufumi; Majima, Yasuo [Kurume Univ., Fukuoka (Japan). School of Medicine

    1984-06-01

    Characteristic finding of CT in the liver of hepatic schistosomiasis japonica were compared with histological changes. The study was made on 7 cases with schistosomatic liver cirrhosis (SLC) and 7 SLC cases with hepatocellular carcinoma (HCC). Characteristic CT findings were high density funicular patterns showing turtle shell appearance (4 cases), high density spotty patterns (3 cases) and both mixed patterns (7 cases). These patterns were not changed by contrast medium study. Funicular and mixed patterns were observed in both lobes of the liver, however, spotty patterns were seen only in the right lobe. It was difficult to distinguish liver tissue surrounded by funicular patterns from HCC lesion. Histopathological study of autopsied livers and CT scanning of thin cut livers revealed that high density funicular patterns represent deposits of calcified schistosomal ova in the fibrous septa.

  10. Distribution and metabolism of galactitol in Euonymus japonica L

    International Nuclear Information System (INIS)

    Boersig, M.R.; Negm, F.B.

    1987-01-01

    Metabolism of [ 14 C]-galactitol was studied in immature and mature leaves of Euonymus japonica L. The highest rates of galactitol metabolism occurred in young expanding leaves. After a 12 h incubation in [ 14 C]-galactitol, the proportions of label remaining in galactitol were 82 and 96% in immature and mature tissues, respectively. A NAD dependent galactitol dehydrogenase reaction was demonstrated in extracts from immature leaves. The product of this reaction was the aldose sugar, galactose. Label from [ 14 C]-galactitol was detected in two unidentified carbohydrates as well as in sucrose, glucose, fructose and galactose. When incubated with [ 14 C]-galactose, immature and mature leaf strips, respectively, accumulated 1 and 22% of the label in the form of galactitol

  11. Development of microsatellite markers from loquat, Eriobotrya japonica (Thunb.) Lindl.

    Science.gov (United States)

    Gisbert, A D; Lopez-Capuz, I; Soriano, J M; Llacer, G; Romero, C; Badenes, M L

    2009-05-01

    Loquat (Eriobotrya japonica) is a minor fruit which has become an interesting alternative into the European fruit industry. This interest resulted in a loquat germplasm collection established at the Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain. Currently, it is the main reservoir of this species outside Asia. We developed and characterized the first 21 polymorphic microsatellite loci from a CT/AG-enriched loquat genomic library. The observed heterozygosity ranged between 0.20 and 1.00, expected heterozygosity ranged between 0.17 and 0.81, three markers were multilocus and eight loci departed significantly from Hardy-Weinberg equilibrium. These markers will facilitate diversity and genetic studies into the species. © 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd.

  12. Antiatherogenic Effect of Camellia japonica Fruit Extract in High Fat Diet-Fed Rats

    OpenAIRE

    Lee, Hyun-Ho; Paudel, Keshav Raj; Jeong, Jieun; Wi, An-Jin; Park, Whoa-Shig; Kim, Dong-Wook; Oak, Min-Ho

    2016-01-01

    Hypercholesterolemia is a well-known etiological factor for cardiovascular disease and a common symptom of most types of metabolic disorders. Camellia japonica is a traditional garden plant, and its flower and seed have been used as a base oil of traditional cosmetics in East Asia. The present study was carried out to evaluate the effect of C. japonica fruit extracts (CJF) in a high fat diet- (HFD-) induced hypercholesterolemic rat model. CJF was administered orally at three different doses: ...

  13. Essential Oil from Cryptomeria japonica Induces Apoptosis in Human Oral Epidermoid Carcinoma Cells via Mitochondrial Stress and Activation of Caspases

    OpenAIRE

    Cha, Jeong-Dan; Kim, Ji-Young

    2012-01-01

    Cryptomeria japonica D. Don (C. japonica) has been used in traditional medicines from Asia for a variety of indications, including liver ailments, and an antitussive, and for its antiulcer activities. We examined the cell viability and apoptosis of KB cells treated with C. japonica essential oil at several concentrations for 12 h by MTT assay, Hoechst-33258 dye staining, DNA fragmentation, flow cytometry (cell cycle), and Western blotting for mitochondria stress, activation of caspases, and p...

  14. Factores de riesgo en el bajo peso al nacer

    Directory of Open Access Journals (Sweden)

    Guillermo Díaz Alonso

    Full Text Available Se realizó un estudio retrospectivo de tipo caso control en el área del Policlínico Docente "Luis A. Turcios Lima" del municipio 10 de Octubre durante el período de 1990 a 1993, con el objetivo de identificar el comportamiento del bajo peso al nacer, establecer la existencia y la intensidad de esta entidad con los factores de riesgo seleccionados, e identificar el grado de influencia individual de dichos factores. La muestra quedó conformada por 92 recién nacidos de bajo peso y 468 con normopeso. Las variables estudiadas fueron la edad materna, la valoración ponderal, las complicaciones obstétricas, las enfermedades asociadas con la gestación, el hábito de fumar y el período intergenésico. Se aplicó el test chi-cuadrado, el odds- -ratio y el de riesgo atribuible en los nacimientos expuestos y en la población total. Todas las variables estuvieron asociadas significativamente con el bajo peso al nacer, y fueron identificadas como factores de riesgo con influencia marcada: la edad materna de 35 años o más, la amenaza de parto prematuro, la rotura prematura de membranas, la toxemia y la diabetes mellitus; con efecto moderado, el bajo peso materno, un período intergenésico menor de 1 año, la hipertensión arterial, el asma bronquial, el hábito de fumar, la sepsis urinaria, y con influencia ligera, la edad materna inferior a los 20 años.

  15. CULTIVOS BAJO CUBIERTA EN EL SURESTE DE ESPAÑA

    Directory of Open Access Journals (Sweden)

    Encarnación Gil Meseguer

    2011-01-01

    Full Text Available En España, más de 62.000 ha regadas se cultivan en ambiente controlado, bajo determinadas cubiertas. Dos tercios se localizan en Andalucía, y más de la cuarta parte, en el Sureste de la Península Ibérica. En el espacio surestino, delimitado por la coincidencia de las isolíneas climáticas (isoyeta de los 400 mm anuales e isoterma de 16ºC de TMA y la línea de costa mediterránea entre Almería y Alicante, sobresalen una serie de estructuras con cubiertas de plásticos y mallas para la producción de cultivos intensivos en las comarcas del Levante Almeriense-Bajo Almanzora, Lorca y Valle Guadalentín, Campo de Cartagena-Mar Menor, Meridional de Alicante-Vega Baja, etc. Los avances tecnológicos aplicados al riego y al ciclo natural de la planta, se incorporan al cultivo forzado bajo cubierta, logrando modificar el ciclo de cultivo, con una amplitud en los calendarios para presentar el producto en los momentos de mayor demanda. El interés de esta agricultura intensiva de invernaderos, mallas y acolchados radica en su mayor productividad, empleo y precio final del producto.

  16. Japanese quail (Coturnix japonica) liver and thyroid gland histopathology as a result of in ovo exposure to the flame retardants tris(1,3-dichloro-2-propyl) phosphate and Dechlorane Plus

    DEFF Research Database (Denmark)

    Jacobsen, Mona L.; Jaspers, Veerle L. B.; Ciesielski, Tomasz M.

    2017-01-01

    that exposure to TDCIPP or the mixture induced hepatic sinusoidal dilatation. No marked effects were seen for DP alone. In addition, the mixture produced divergence of thyroid gland follicles and proliferation of follicular cells. Our study is the first demonstrating histopathological alterations as a result...

  17. Suppressive effects of long-term exposure to P-nitrophenol on gonadal development, hormonal profile with disruption of tissue integrity, and activation of caspase-3 in male Japanese quail (Coturnix japonica)

    OpenAIRE

    Ahmed, Eman; Nagaoka, Kentaro; Fayez, Mostafa; Abdel-Daim, Mohamed M.; Samir, Haney; Watanabe, Gen

    2015-01-01

    P-Nitrophenol (PNP) is considered to be one of nitrophenol derivatives of diesel exhaust particles. PNP is a major metabolite of some organophosphorus compounds. PNP is a persistent organic pollutant as well as one of endocrine-disrupting compounds. Consequently, bioaccumulation of PNP potentiates toxicity. The objectives of the current study were to assess in vivo adverse effects of long-term low doses of PNP exposure on reproductive system during development stage. Twnety-eight-day-old male...

  18. Age-dependent change in the morphology of nucleoli and methylation of genes of the Nucleolar Organizer Region in Japanese quail Coturnix japonica) model (Temminck and Schlegel, 1849) (Galliformes: Aves).

    Science.gov (United States)

    Andraszek, Katarzyna; Gryzińska, Magdalena; Wójcik, Ewa; Knaga, Sebastian; Smalec, Elżbieta

    2014-01-01

    Nucleoli are the product of the activity of nucleolar organizer regions (NOR) in certain chromosomes. Their main functions are the formation of ribosomal subunits from ribosomal protein molecules and the transcription of genes encoding rRNA. The aim of the study was to determine the shape of nucleoli and analyse methylation in the gene RN28S in the spermatocytes of male Japanese quail (Coturnixjaponica) in two age groups. Nucleoli were analysed in cells of the first meiotic prophase. Their number and shape were determined and they were classified as regular, irregular or defragmented. In the cells of the young birds no defragmented nucleoli were observed, with regular and irregular nucleoli accounting for 97% and 3%, respectively. In the cells of older birds no regular nucleoli were observed, while irregular and defragmented nucleoli accounted for 37% and 67%, respectively. MSP (methylation-specific PCR) showed that the gene RN28S is methylated in both 15-week-old and 52-week-old quails. In recent years an association has been established between nucleolus morphology and cellular ageing processes.

  19. Blood clinical-chemical parameters and feeding history in growing Japanese quail (Coturnix japonica) chicks exposed to Tris(1,3-dichloro-2-propyl) phosphate and Dechlorane Plus in ovo

    DEFF Research Database (Denmark)

    Jacobsen, Mona Lykke; Jaspers, Veerle L B; Ciesielski, Tomasz M.

    2017-01-01

    . The 8 days consuming the new feed played a significant role on 12 of 18 BCCPs measured. Consequently, it is recommended that dietary habits need to be considered when investigating the physiological impact of contaminants using BCCPs as biomarkers in growing nestlings both in controlled in vivo...

  20. resources 1..1

    Indian Academy of Sciences (India)

    http://www.ias.ac.in/jgenet/OnlineResources/90/e34.pdf. Morinha F., Carvalho M., Ferro A., Guedes-Pinto H., Rodrigues R. and Bastos E. 2011 Molecular sexing and analysis of CHD1-Z and. CHD1-W sequence variations in wild common quail (Coturnix c. coturnix) and domesticated Japanese quail (Coturnix c. japonica).

  1. Is gastrointestinal plasticity in king quail (Coturnix chinensis) elicited by diet-fibre or diet-energy dilution?

    Science.gov (United States)

    Williamson, Sean A; Jones, Stephanie K Courtney; Munn, Adam J

    2014-06-01

    Phenotypic plasticity of organ size allows some animals to manage fluctuations of resource quality or availability. Here, we examined the phenotypic plasticity of the gastrointestinal tract of king quail (Coturnix chinensis) in a diet-fibre manipulation study. Quail were offered either a control low-fibre (high-quality) food (8.5% neutral-detergent fibre; NDF), or one of two experimental diets of higher fibre contents of 16% NDF (i.e. low-quality food). To examine whether phenotypic plasticity of organ size was associated with the fibre content per se, or as a consequence of diluting the diet energy contents by adding fibre, one of the high-fibre feeds was 'balanced' with additional energy to match that of the low-fibre control diet. Total empty dry mass of the gastrointestinal tract was significantly heavier among birds offered the unbalanced high-fibre diet as compared with those offered the control diet, with birds offered the fibrous but energy-balanced diet having guts of intermediate size. The heavier entire-gut mass (dry) of quail offered the unbalanced high-fibre diet was associated mainly with these birds having significantly heavier gizzards. Notably, the larger gizzard in the birds offered the unbalanced high-fibre diet was associated with marked increases in their metabolisability (digestion) of diet fibre. Our findings suggest that the available energy in the diet may be more important for eliciting phenotypic changes in the gut of these herbivorous birds rather than simple physical effects of diet fibre on feed intakes or on muscular compensation to fibrous ingesta. © 2014. Published by The Company of Biologists Ltd.

  2. Enzyme expression in indica and japonica rice cultivars under saline stress=Expressão de enzimas em cultivares de arroz indica e japonica sob estresse salino

    Directory of Open Access Journals (Sweden)

    Cristina Rodrigues Mendes

    2012-10-01

    Full Text Available The southern State of Rio Grande do Sul (RS is the main rice producer in Brazil with a 60% participation of the national production and 86% participation of the region. Rice culture irrigation system is done by flooding, which leads to soil salinization, a major environmental constraint to production since it alters the plants’ metabolism exposed to this type of stress. The indica cultivar, widely used in RS, has a higher sensitivity to salinity when compared to that of the japonica cultivar in other physiological aspects. Current research analyzes enzymes expression involved in salt-subjected indica and japonica rice cultivars’ respiration. Oryza sativa L. spp. japonica S.Kato (BRS Bojuru, IAS 12-9 Formosa and Goyakuman and Oryza sativa L. spp. indica S. Kato (BRS Taim-7, BRS Atalanta and BRS Querencia were the cultivars employed. Seedlings were transferred to 15 L basins containing 50% Hoagland nutrient solution increased by 0, 25, 50, 75 and 100 mM NaCl, and collected at 14, 28 and 42 days after transfer (DAT. Plant tissues were macerated and placed in eppendorf tubes with Scandálios extractor solution. Electrophoresis was performed in 7% of the polyacrylamide gels in vertical vats. Bands were revealed for the following enzymes systems: esterase, alcohol dehydrogenase, phosphoglucoisomerase, malate dehydrogenase, malic enzyme and alpha amylase. The enzymes expression was greater in subspecies japonica, with more intense bands in proportion to salinity increase. Results show that enzyme systems are involved in the salinity defense mechanisms in O. sativa spp. japonica cultivar.O Estado do Rio Grande do Sul (RS destaca-se como principal produtor de arroz, participando com 60% da produção nacional e 86% da regional. O sistema de irrigação da cultura é por inundação, que induz o solo à salinização, um dos maiores limitadores ambientais à produção, alterando o metabolismo da plantas expostas a este tipo de estresse. As cultivares

  3. Radionuclide study on hepatic blood flow in Schistosomiasis Japonica

    Energy Technology Data Exchange (ETDEWEB)

    Okada, Junichi; Uchiyama, Guio; Hayakawa, Kazushige; Hayashi, Sanshin; Araki, Tsutomu; Arai, Takao; Iuchi, Masahiko

    1986-11-01

    Schistosomiasis Japonica is a regional disease found in elderly people who were living in once-endemic areas in Japan. Yamanashi was one of these areas until 1970, since when no newly infected patients were reported. The disease is characteristic of developing irreversible interstitial fibrosis of the liver, where parasites migrate and lay eggs. Portal hypertension, esophageal varices and hepatocellular carcinomas are the common features of the results. In order to estimate patient's hepatic blood flow, radionuclide angiography of the liver with the use of 10 - 15 millicuries of Tc-99m phytate were performed prior to the conventional multiview imaging. Twenty-two patients with schistosomiasis and twelve adults without evidence of liver disease were studied. A time-activity curve of the right lobe of the liver was generated by a computer, and the ratio of arterial blood flow to portal blood flow was calculated. As a result, a good correlation was found between the arterial to portal blood flow ratio and the grade of hepatic fibrosis verified by laparoscopic biopsy. The development of esophageal varices were likely to correlate well with the blood flow ratio rather than scores on the conventional static liver and spleen scintigram. The study was useful for evaluating patient's clinical stages and prognosis.

  4. [Luring effect of the fermented Laminaria japonica to Oncomelania hupensis].

    Science.gov (United States)

    Ma, An-ning; Ni, Hong; Wang, Wan-xian; Zhang, Yun; Geng, Peng

    2010-02-01

    To study the attraction effect of the food attractants on Oncomelania hupensis. Oncomelania snail food was prepared with the fermented kelp (Laminaria japonica) mixed with corn starch. Snails were fed with the food and kept for 12, 24, 36, and 48 h at 15, 25, 35 degrees C respectively. Meanwhile, snail-killing effect was tested by granules containing jack-in-the-pulpit (Arisama heterophyllum) with or without the fermented kelp under the condition of 25 degrees C, 30% or 60% soil humidity. The snail-attracting rate of the fermented kelp was affected by the temperature, highest under 25 degrees C and lowest under 35 degrees C at any time point, with a rate of 80.3% in 48 h at 25 degrees C which was higher than that of the control (17.0%) (Pjack-in-the-pulpit with fermented kelp (85.3%) was higher than that of the group without fermented kelp (26.8%) (P<0.05). The mortality under 60% of soil humidity was higher than that under 30% humidity (P<0.01). The fermented kelp shows a strong luring effect to the Oncomelania snails.

  5. Emission of floral volatiles from Mahonia japonica (Berberidaceae).

    Science.gov (United States)

    Picone, Joanne M; MacTavish, Hazel S; Clery, Robin A

    2002-07-01

    Flowering Mahonia japonica plants were subjected to controlled environments and the floral volatiles emitted from whole racemes (laterals) were trapped by Porapak Q adsorbent and analysed by GC-FID. An experiment with photoperiods of 6 and 9 h at constant temperature (10+/-1 degrees C) demonstrated that photoperiod was the stimulus for enhanced emission of most volatiles. Small quantitative differences in emitted fragrance composition were observed between light and dark periods and between plants acclimatised to different photoperiods. Maximum rates of emission occurred in the middle of the light period; aromatic compounds (benzaldehyde, benzyl alcohol and indole) displayed a more rapid increase and subsequent decline compared with monoterpenes (cis- and trans-ocimene and linalool). When the photoperiod was extended from 6 to 9 h, maximum rates of emission continued throughout the additional 3 h. Total emission (microg/h) of volatiles was 2-fold greater in the day-time (DT) (39.7 microg/h) compared with the night-time (NT) (19.8 microgg/h) under a 6 h photoperiod and was not significantly different from total emission under a 9 h photoperiod.

  6. Protective Effect of Laminaria japonica with Probiotics on Murine Colitis

    Directory of Open Access Journals (Sweden)

    Seok-Jae Ko

    2014-01-01

    Full Text Available Inflammatory bowel disease (IBD is a chronically relapsing inflammatory disorder of the gastrointestinal tract. Most IBD treatments are unsatisfactory; therefore, various dietary supplements have emerged as promising interventions. Laminaria japonica (LJ is an edible seaweed used to regulate digestive symptoms. Probiotics have been reported to improve digestive problems and their simultaneous administration with seaweeds has been shown to produce synergistic therapeutic effects. Here, we investigated the effect of LJ combination with probiotics on dextran sodium sulfate-induced colitis model in mice. Aqueous LJ extracts (LJE at doses from 100 to 300 mg/kg and probiotics at a dose of 300 mg/kg were orally administered for 7 days. Body weight, colon length, histological score, macroscopic damage, and the levels of cytokines IFN-γ, IL-1β, IL-6, IL-10, IL-12 (P40, IL-12 (P70, IL-17, and TNF-α were assessed. LJE alone caused a significant improvement of colitis signs such as colon length, histological score, and IL-1β and IL-6 production. LJE and probiotics demonstrated a synergistic effect by the histological score and levels of IL-1β, IL-6, and IL-12 (P40 but not IFN-γ, IL-10, and IL-12 (P70. In conclusion, LJE was effective in inducing protection against colitis in mice and acted synergistically with probiotics.

  7. Characteristic odor components of essential oils from Eurya japonica.

    Science.gov (United States)

    Motooka, Ryota; Usami, Atsushi; Nakahashi, Hiroshi; Koutari, Satoshi; Nakaya, Satoshi; Shimizu, Ryoyu; Tsuji, Kaoru; Marumoto, Shinsuke; Miyazawa, Mitsuo

    2015-01-01

    The chemical compositions of essential oils from the flower and aerial parts (i.e., leaf and branch) of Eurya japonica were determined and quantified using gas chromatography-mass spectrometry (GC-MS). A total of 87 and 50 compounds were detected in the oils from the flower and aerial parts, respectively. The main compounds of the flower oil were linalool (14.0%), (9Z)-tricosene (12.0%), and nonanal (7.4%). In the oil from the aerial parts, linalool (37.7%), α-terpineol (13.5%), and geraniol (9.6%) were detected. In the oils from the flower and aerial parts, 13 and 8 aroma-active compounds were identified by GC-olfactometry (GC-O) analysis, respectively. The key aroma-active compounds of the flower oil were heptanal [fatty, green, flavor dilution (FD) = 128, odor activity value (OAV) = 346], nonanal (sweet, citrus, FD = 128, OAV = 491), and eugenol (sweet, spicy, FD = 64, OAV = 62): in the oil from the aerial parts, the key aroma-active compounds were linalool (sweet, citrus, FD = 64, OAV = 95), (E)-β-damascenone (sweet, FD = 256, OAV = 4000), and (E)-β-ionone (floral, violet, FD = 128, OAV = 120). This study revealed that nonanal and eugenol impart the sweet, citrus, and spicy odor of the flower oil, while (E)-β-damascenone and (E)-β-ionone contribute the floral and sweet odor of the oil from the aerial parts.

  8. Radionuclide study on hepatic blood flow in Schistosomiasis Japonica

    International Nuclear Information System (INIS)

    Okada, Junichi; Uchiyama, Guio; Hayakawa, Kazushige; Hayashi, Sanshin; Araki, Tsutomu; Arai, Takao; Iuchi, Masahiko.

    1986-01-01

    Schistosomiasis Japonica is a regional disease found in elderly people who were living in once-endemic areas in Japan. Yamanashi was one of these areas until 1970, since when no newly infected patients were reported. The disease is characteristic of developing irreversible interstitial fibrosis of the liver, where parasites migrate and lay eggs. Portal hypertension, esophageal varices and hepatocellular carcinomas are the common features of the results. In order to estimate patient's hepatic blood flow, radionuclide angiography of the liver with the use of 10 - 15 millicuries of Tc-99m phytate were performed prior to the conventional multiview imaging. Twenty-two patients with schistosomiasis and twelve adults without evidence of liver disease were studied. A time-activity curve of the right lobe of the liver was generated by a computer, and the ratio of arterial blood flow to portal blood flow was calculated. As a result, a good correlation was found between the arterial to portal blood flow ratio and the grade of hepatic fibrosis verified by laparoscopic biopsy. The development of esophageal varices were likely to correlate well with the blood flow ratio rather than scores on the conventional static liver and spleen scintigram. The study was useful for evaluating patient's clinical stages and prognosis. (author)

  9. Growth modeling of Cryptomeria japonica by partial trunk analysis

    Directory of Open Access Journals (Sweden)

    Vinícius Morais Coutinho

    2017-06-01

    Full Text Available This study aimed to evaluate the growth pattern of Cryptomeria japonica increment (L. F. D. Don. and to describe the probability distribution in stands stablished at the municipality of Rio Negro, Paraná State. Twenty trees were sampled in a 34 years-old stand, with 3 m x 2 m spacing. Wood disks were taken from each tree at 1.3 m above the ground (DBH to perform partial stem analysis. Diameter growth series without bark were used to generate the average cumulative growth curves for DBH (cm, mean annual increment (MAI and current annual increment (CAI. From the increment data, the frequency distribution was evaluated by means of probability density functions (pdfs. The mean annual increment for DBH was 0.78 cm year-1 and the age of intersection of CAI and MAI curves was between the 7th and 8th years. It was found that near 43% of the species increments are concentrated bellow 0.5 cm. The results are useful to define appropriate management strategies for the species for sites similar to the studying regions, defining for example ages of silvicultural intervention, such as thinning.

  10. De Novo Assembly and Characterization of Sophora japonica Transcriptome Using RNA-seq

    Directory of Open Access Journals (Sweden)

    Liucun Zhu

    2014-01-01

    Full Text Available Sophora japonica Linn (Chinese Scholar Tree is a shrub species belonging to the subfamily Faboideae of the pea family Fabaceae. In this study, RNA sequencing of S. japonica transcriptome was performed to produce large expression datasets for functional genomic analysis. Approximate 86.1 million high-quality clean reads were generated and assembled de novo into 143010 unique transcripts and 57614 unigenes. The average length of unigenes was 901 bps with an N50 of 545 bps. Four public databases, including the NCBI nonredundant protein (NR, Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG, and the Cluster of Orthologous Groups (COG, were used to annotate unigenes through NCBI BLAST procedure. A total of 27541 of 57614 unigenes (47.8% were annotated for gene descriptions, conserved protein domains, or gene ontology. Moreover, an interaction network of unigenes in S. japonica was predicted based on known protein-protein interactions of putative orthologs of well-studied plant genomes. The transcriptome data of S. japonica reported here represents first genome-scale investigation of gene expressions in Faboideae plants. We expect that our study will provide a useful resource for further studies on gene expression, genomics, functional genomics, and protein-protein interaction in S. japonica.

  11. Microcosm investigation of growth and phytoremediation potential of Azolla japonica along nitrogen gradients.

    Science.gov (United States)

    Park, Hun; Song, Uhram

    2017-10-03

    Although Azolla species are among the most promising plants for use in phytoremediation, more studies on their growth and nitrogen (N) uptake along the N gradients of growing media are required. In this study, N concentration-dependent growth in growing media and phosphorus (P) and N accumulation by Azolla japonica were studied by estimating direct N uptake from media by molybdenum-iron proteins. The doubling time of A. japonica was less than a week, regardless of the N concentration (0, 5, and 25 mg N/L) present in the growth media, indicating that this plant is suitable for remediation. Plants showed a high uptake of P, probably via plant-bacteria symbiosis, indicating their potential for effective P remediation. A. japonica also showed more than 4% N content regardless of the treatment and accumulated more than 40 mg of N per microcosm in 3 weeks. iron and molybdenum levels in plants were strongly associated with N fixation, and N uptake from media was estimated to be more than 25 mg per microcosm in 3 weeks, indicating that A. japonica has N remediation potential. As A. japonica is a rapidly growing plant, capable of efficient P and N remediation, it has great potential for use in phytoremediation of nutrient-enriched waters such as agricultural or urban wastewater and eutrophicated aquatic ecosystems.

  12. Aulas-laboratorios de bajo costo, usando TIC

    Directory of Open Access Journals (Sweden)

    Silvia E. Calderón

    2015-01-01

    Full Text Available En este trabajo se presentan los resultados de una propuesta educativa orientada a promover el desarrollo de un pensamiento critico y un mayor interes por las ciencias experimentales. Con este fin desarrollamos propuestas de proyectos educativos susceptibles de ser destinadas a las aulas y laboratorios de las escuelas secundarias y primeros anos de la universidad, que resaltan los aspectos metodologicos de las ciencias. Aqui, realizamos una compilacion de varios proyectos, que ilustran formas de incorporar las tecnologias de la informacion y la comunicacion (TIC en diversos experimentos de ciencias, muchos de ellos publicados individualmente anteriormente, y que en conjunto se pueden utilizar para implementar un aula-laboratorio de bajo costo. Con TIC hacemos referencia a la convergencia de computadoras, sistemas audiovisuales, Internet, telefonia, y diversos equipos que se integran con algunos de ellos. Los proyectos intentan integrar areas como fisica, matematica, quimica, informatica, arte, etc. y apuntan a que los estudiantes puedan responder a las preguntas: .Como sabemos esto?, .Por que creemos en aquello? Preguntas que ilustran la naturaleza del pensamiento cientifico. Nuestra contribucion mas significativa es haber desarrollado ≪aulas-laboratorios≫ de muy bajo costo, usando TIC. Con el advenimiento de programas como ≪Una Laptop por Nino≫ que se estan implementando en varios paises de Latinoamerica, resulta oportuno utilizar este recurso como base para generar laboratorios de bajo costo, que creemos pueden ser una herramienta util para mejorar el aprendizaje de las ciencias, incentivar vocaciones y contribuir a desarrollar un pensamiento critico, a la par de desarrollar habilidades con el uso de las TIC que pueden ser de utilidad en diversos ambitos academicos y laborales.

  13. [Epiphytic algae from Bajo Pepito, Isla Mujeres, Quintana Roo, Mexico].

    Science.gov (United States)

    Quan-Young, L I; Díaz-Martín, M A; Espinoza-Avalos, J

    2006-06-01

    A total of 96 epiphytic algae species were identified from Bajo Pepito, Quintana Roo, México. 60.4% (58) belonged to the Rhodophyta, 19.79% (19) to the Phaeophyta, 16.6% (16) to the Chlorophyta and 3.1% (3) to the Cyanophyta; 49 species (50.5%) were found only in one month, while Heterosiphonia crispella was found in all of the sampled months. That species provided the largest contribution to the biomass of epiphytes. During January we registered the greater biommass and richness of epiphytes species, coincidently with high values of host species cover and rainfall.

  14. Desafío con wssv bajo fluctuaciones de temperatura

    OpenAIRE

    Sonnenholzner, Stanislaus

    2002-01-01

    Desafío con WSSV bajo fluctuaciones de temperatura En trabajos anteriores sobre el efecto de la temperatura del agua sobre la supervivencia del camarón infectado con el virus de la Mancha Blanca, se demostró que una temperatura constante de 33ºC por 8 días genera una respuesta inmune en el camarón correlacionada a una supervivencia superior al 90%, lo cual se tradujo posteriormente en una propuesta práctica de cultivo de camarón en estanques con invernaderos, actualmente en evaluación e...

  15. JST Thesaurus Headwords and Synonyms: Dioscorea japonica Thunb. [MeCab user dictionary for science technology term[Archive

    Lifescience Database Archive (English)

    Full Text Available MeCab user dictionary for science technology term Dioscorea japonica Thunb. 名詞 一般 *... * * * ヤマノイモ ヤマノイモ ヤマノイモ Thesaurus2015 200906042151087664 C LS06/LS63/LS72 UNKNOWN_2 Dioscorea japonica Thunb .

  16. JST Thesaurus Headwords and Synonyms: Dioscorea japonica [MeCab user dictionary for science technology term[Archive

    Lifescience Database Archive (English)

    Full Text Available MeCab user dictionary for science technology term Dioscorea japonica 名詞 一般 * * * * ...ヤマノイモ ヤマノイモ ヤマノイモ Thesaurus2015 200906042151087664 C LS06/LS63/LS72 UNKNOWN_2 Dioscorea japonica

  17. A tale of two seagrasses: Comparing the science and management of Zostera marina and Zostera japonica in the Pacific Northwest

    Science.gov (United States)

    On the Pacific coast of North America, at least two congeners of Zostera occur: native Zostera marina, and introduced, Z. japonica. Z. japonica is considered “invasive” and therefore, ecologically and economically harmful by some, while others consider it benign or perhaps benef...

  18. JST Thesaurus Headwords and Synonyms: Wasabia japonica [MeCab user dictionary for science technology term[Archive

    Lifescience Database Archive (English)

    Full Text Available MeCab user dictionary for science technology term Wasabia japonica 名詞 一般 * * * * ワサ...ビ ワサビ ワサビ Thesaurus2015 200906097830728309 C LS06/LS72 UNKNOWN_2 Wasabia japonica

  19. Absorption and translocation of 15N in Japonica (Hinohikari) and Indica (Hadsaduri) rice varieties

    International Nuclear Information System (INIS)

    Islam, N.; Inagaki, S.; Chishaki, N.; Horiguchi, T.

    1997-01-01

    The absorption and translocation of 15 N-labeled nitrogen (N) applied as three N levels of ammonium nitrate at the stages of panicle initiation (PI) and heading (HD) were compared between a japonica rice variety (var. Hinohikari) and a tall indica rice variety (var. Hadsaduri) by growing them hydroponically. With the supply of low N level, 15 N absorption by the japonica variety was larger, but at medium and high N levels, the tall indica variety absorbed larger amounts of 15 N at both stages. However, the amount of 15 N partitioned to the panicles at maturity was considerably smaller in the indica variety, since dry matter allocation to the panicles was also smaller in this variety. The tall indica variety showed a considerable loss of 15 N from heading to maturity at the high N-level unlike the japonica variety. (author)

  20. Bajo materialismo y surrealismo. El debate Bataille-Breton

    Directory of Open Access Journals (Sweden)

    Noelia Denise Dunan

    2015-01-01

    Full Text Available Hacia 1923 Georges Bataille era un joven desconocido que comenzaba a entablar amistades y también enemistades en el núcleo del movimiento surrealista e intelectual de París. Alentado por algunos de aquellos vínculos, lee a Mauss y Durkheim, Nietzsche y Freud y, comienza a delinear un pensamiento que se plasmará en la creación de Documents. Resulta difícil comprender la relevancia de esta revista y la producción batailleana sin referir a sus primeras lecturas y al debate que tempranamente entabló con el máximo exponente del movimiento surrealista, André Breton. Bataille coordinó allí un grupo de disidentes y convirtió la publicación en una máquina contra el surrealismo. El presente artículo desarrolla la formación de Bataille para hacer hincapié en la categoría de bajo materialismo construida en tensión entre el idealismo surrealista y los distintos materialismos. Así puede verse que Documents y el bajo materialismo conjugan las preocupaciones que perseguirá toda su vida.

  1. Perubahan Sosial Pada Komunitas Suku Bajo Di Pesisir Teluk Tomini

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    Muhammad Obie

    2016-06-01

    Full Text Available Abstract: Society and culture of human being always change as an ever –present phenomena in the world. A distinction is sometimes made then between processes of change within the social structure, which serve in part to maintain the structure, and, even the processes itself can be slow or fast through evolusion and revolusion.   One of the factors that quicken the social change process is the government interventions. This study aims at analyzing the social change on the Bajo Tribe community as the impact of various government programs at Tomini bay coastal. This research used strategy of historical sociology. The kinds of data collected were primer and secondary data that were analyzed by using qualitative approac. The result showed that various   government programs at Tomini bay, such as designating conservation area, granting concession license to private sectors through forest concession right (FCR, indefeasible right of use (IRU, and fishing industry at the bay had caused the loss of access of the Bajo tribe community to coastal and marine resources area. Various interventions of government programs had implication on resettlement of the Bajo tribe community, causing the Bajo tribe divided into sea Bajo and land Bajo. The loss of access of the Bajo tribe to coastal and marine resources caused traditional institution weaken which made its philosophy of living in harmony with nature, and  conservation ethics fade away, its local wisdom, and self identity lose, and its social capital destructed.  الملخص: كان المجتمع وحضارته متغيّرين في أيّ كان المجتمع وهذه الحضارة. ويكون الفرق في كون هذا التغيّر. قد تكون عملية التغيّر متمهّلة أو سريعة وقد تكون عن طريق التطوّر والثّورة. وأحد العناصر المسرعة في عملية هذا التغيّر هو تدخّل الحكومة. حاولت هذه

  2. Catalytic pyrolysis of Laminaria japonica over nanoporous catalysts using Py-GC/MS

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    Jeon Jong-Ki

    2011-01-01

    Full Text Available Abstract The catalytic pyrolysis of Laminaria japonica was carried out over a hierarchical meso-MFI zeolite (Meso-MFI and nanoporous Al-MCM-48 using pyrolysis gas chromatography/mass spectrometry (Py-GC/MS. The effect of the catalyst type on the product distribution and chemical composition of the bio-oil was examined using Py-GC/MS. The Meso-MFI exhibited a higher activity in deoxygenation and aromatization during the catalytic pyrolysis of L. japonica. Meanwhile, the catalytic activity of Al-MCM-48 was lower than that of Meso-MFI due to its weak acidity.

  3. Colon cancer proliferating desulfosinigrin in wasabi (Wasabia japonica).

    Science.gov (United States)

    Weil, Marvin J; Zhang, Yanjun; Nair, Muraleedharan G

    2004-01-01

    A reduced incidence of different types of cancer has been linked to consumption of Brassica vegetables, and there is evidence that glucosinolates (GSLs) and their hydrolysis products play a role in reducing cancer risk. Wasabi (Wasabia japonica) and horseradish (Armoracia rusticana), both Brassica vegetables, are widely used condiments both in Japanese cuisine and in the United States. Desulfosinigrin (DSS) (1) was isolated from a commercially available wasabi powder and from fresh wasabi roots. Sinigrin (2) was isolated from horseradish roots. DSS and sinigrin were evaluated for their inhibitory effects on cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) enzymes, on lipid peroxidation, and on the proliferation of human colon (HCT-116), breast (MCF-7), lung (NCIH460), and central nervous system (CNS, SF-268) cancer cell lines. DSS did not inhibit COX enzymes or lipid peroxidation at 250 microg/ml. Sinigrin inhibited lipid peroxidation by 71% at 250 microg/ml. However, DSS promoted the growth of HCT-116 (colon) and NCI H460 (lung) human cancer cells as determined by the MTT assay in a concentration-dependent manner. At 3.72 microg/ml, a 27% increase in the number of viable human HCT-116 colon cancer cells was observed; the corresponding increases at 7.50 and 15 microg/ml were 42 and 69%, respectively. At 60 microg/ml, DSS doubled the number of HCT-16 colon cancer cells. For NCI H460 human lung cancer cells, DSS at 60 microg/ml increased the cell number by 20%. Sinigrin showed no proliferating effect on the tumor cells tested. This is the first report of the tumor cell-proliferating activity by a desulfoglucosinolate, the biosynthetic precursor of GSLs found in Brassica spp.

  4. Isolation of antiosteoporotic compounds from seeds of Sophora japonica.

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    Hossam M Abdallah

    Full Text Available Chemical investigation of Sophora japonica seeds resulted in the isolation of seven metabolites identified as: genistin (1, sophoricoside (2, sophorabioside (3, sophoraflavonoloside (4, genistein 7,4'-di-O-β-D-glucopyransoide (5, kaempferol 3-O-α-L-rhamnopyranosyl(1 → 6β-D-glucopyranosyl(1 → 2β-D-glucopyranoside (6 and rutin (7. Compounds 1, 2 and 5 showed significant estrogenic proliferative effect in MCF-7 cell in sub-cytotoxic concentration range. Compounds 1 and 2 showed minimal cell membrane damaging effect using LDH leakage assay. Accordingly, compound 2 (sophoricoside, (SPH was selected for further in-vivo studies as a potential anti-osteoporosis agent. The anti-osteoporotic effect of SPH was assessed in ovarectomized (OVX rats after oral administration (15 mg/kg and 30 mg/kg for 45 days compared to estradiol (10 µg/kg as a positive control. Only in a dose of 30 mg/kg, SPH regained the original mechanical bone hardness compared to normal non-osteoporotic group. However, SPH (15 mg/kg significantly increased the level of alkaline phosphatase (ALP to normal level. Treatment with SPH (30 mg/kg increased the level of ALP to be higher than normal group. SPH (15 mg/kg did not significantly increase the serum level of osteocalcin (OC compared to OVX group. On the other hand, treatment with SPH (30 mg/kg significantly increased the level of OC to 78% higher than normal non-ovarectomized animals group. In addition, SPH (15 mg/kg decreased the bone resorption marker, acid phosphatase (ACP to normal level and SPH (30 mg/kg further diminished the level of serum ACP. Histopathologically, sophoricoside ameliorated the ovarectomy induced osteoporosis in a dose dependent manner. The drug showed thicker bony trabeculae, more osteoid, and more osteoblastic rimming compared to OVX group.

  5. Sterilization of Hulecoeteomyia japonica japonica (=Aedes japonicus japonicus) (Theobald, 1901) by high-energy photon irradiation: implications for a sterile insect technique approach in Europe

    International Nuclear Information System (INIS)

    Balestrino, F.; Mathis, A.; Veronesi, E.; Lang, S.

    2017-01-01

    Hulecoeteomyia japonica japonica (=Aedes japonicus japonicus) (Diptera: Culicidae) (Theobald 1901), a container breeding invasive species in North America and Europe, is attracting particular attention for its high local abundances and possible roles in the transmission of human and animal pathogens. The preferential habitats of this species are forested and bushy areas, which renders control measures extremely inefficient. Use of the sterile insect technique (SIT) may contribute to the implementation of area-wide integrated pest management strategies, as has been successfully proven with other aedine mosquito species. The present study investigates the effects of irradiation at a dose of 40 Gy on fitness parameters in H. j. japonica. Irradiation was performed on 16–24- h-old pupae from a colonized strain (PA) using a True- Beam linear accelerator. Males from the PA strain were crossed with females of the same colony or with field-collected females. Irradiation induced a slight increase in mortality in male pupae, but did not alter the survival and mating abilities of emerging adult males. Rates of blood feeding and fertility were lower when PA strain males were kept with field-collected females rather than PA females. Irradiated males induced reductions in fertility (residual fertility: 2.6%) and fecundity in mated females. The data indicate that the SIT is a suitable technique to enhance the control of this species. (author)

  6. bajo el bosque en Costa Rica. 1980-2000

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    Rafael Ocampo

    2007-01-01

    Full Text Available La ipecacuana o raicilla, hierba nativa de América, es la única planta medicinal del trópico húmedo de Costa Rica cultivada bajo el bosque. Es cultivada en la región Huetar Norte, limítrofe con Nicaragua; la raíz seca de ipecacuana se ha comercializado en Costa Rica como materia prima para la industria farmacéutica internacional desde principios del siglo XX. De acuerdo con las estadísticas oficiales, las exportaciones de raicilla desde Costa Rica en los últimos 20 años han significado un promedio de 64 t año-1. Este trabajo resume actividades relacionadas con la descripción y cultivo de la planta, así como algunos de los aspectos agroecológicos de su cultivo.

  7. Factores de riesgo en el bajo peso al nacer

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    Guillermo Díaz Alonso

    1995-06-01

    Full Text Available Se realizó un estudio retrospectivo de tipo caso control en el área del Policlínico Docente "Luis A. Turcios Lima" del municipio 10 de Octubre durante el período de 1990 a 1993, con el objetivo de identificar el comportamiento del bajo peso al nacer, establecer la existencia y la intensidad de esta entidad con los factores de riesgo seleccionados, e identificar el grado de influencia individual de dichos factores. La muestra quedó conformada por 92 recién nacidos de bajo peso y 468 con normopeso. Las variables estudiadas fueron la edad materna, la valoración ponderal, las complicaciones obstétricas, las enfermedades asociadas con la gestación, el hábito de fumar y el período intergenésico. Se aplicó el test chi-cuadrado, el odds- -ratio y el de riesgo atribuible en los nacimientos expuestos y en la población total. Todas las variables estuvieron asociadas significativamente con el bajo peso al nacer, y fueron identificadas como factores de riesgo con influencia marcada: la edad materna de 35 años o más, la amenaza de parto prematuro, la rotura prematura de membranas, la toxemia y la diabetes mellitus; con efecto moderado, el bajo peso materno, un período intergenésico menor de 1 año, la hipertensión arterial, el asma bronquial, el hábito de fumar, la sepsis urinaria, y con influencia ligera, la edad materna inferior a los 20 años.A case-control retrospective study was carried out at "Luis A. Turcios Lima" Teaching Polyclinics form 10 de Octubre municipality during the period of 1990-1993, with the aim of identifying the behaviour of low birth weight, establishing the occurrence and intensity of this entity with selected risk factors, and identifying the degree of individual influence of such factors. The sample consisted of 92 low birth weight infants and 468 with normal weight. Variables studied were: maternal age, weight appraisal, and obstetric complications, diseases associated with pregnancy, smoking habit, and the

  8. [Mechanisms of subspecies differentiation in a filial generation of rice indica-japonica hybridization under different ecological conditions].

    Science.gov (United States)

    Wang, He-Tong; Jin, Feng; Jiang, Yi-Jun; Lin, Qing-Shan; Xu, Hai; Chen, Wen-Fu; Xu, Zheng-Jin

    2013-11-01

    Indica-japonica hybridization is one of the most important breeding methods in China, whereas identifying subspecies differentiation mechanisms is the key in indica-japonica hybridization breeding. By using InDels (Insert/Deletion) and ILPs (Intron Length Polymorphism), an analysis was made on the F6 populations derived from the hybridization of indica-japonica (Qishanzhan/Akihikari) planted in Liaoning and Guangdong provinces and generated by bulk harvesting (BM), single-seed descent methods (SSD), and pedigree method (PM). No segregation distortion was observed for the BM and SSD populations. The frequency distribution of japonica kinship percentage (Dj) was concentrated in 40%-60%. The PM populations in the two provinces presented indica-deviated distribution (30%-55%), with significant difference between Guangdong (38%) and Liaoning (42%). In addition, there was a significant positive correlation between the Dj and the kinship of functional gene regions in the BM and SSD populations. However, part of the positive correlation was broken in the PM populations that showed a regular distribution in the genotype patterns of indica and japonica loci. The above results demonstrated that artificial selection could be the main factor affecting the population differentiation in indica-japonica hybridization, and, with the synergistic effect of natural selection, induced the phenomenon of segregation distortion. There existed a close relationship between the differentiation of subspecies and the important agronomic traits, which could be the main reason why indica-japonica hybridiation breeding could not achieve the expected effect of combining the two subspecies advantages.

  9. Innate olfactory responses of Asobara japonica toward fruits infested by the invasive spotted wing Drosophila

    Science.gov (United States)

    Insect parasitoids are often manipulated to improve biological control programs for various arthropod pests. Volatile compounds can be a relevant cue used by most parasitoid hymenoptera for host or host microhabitat location. We studied olfactory responses of the braconid Asobara japonica Belokobyls...

  10. Dwarf eelgrass, Zostera japonica: a malevolent, benevolent, or benign invasive ecosystem engineer?

    Science.gov (United States)

    Dwarf eelgrass, Zostera japonica, is an introduced ecosystem engineering species first reported on the US west coast in 1957. In some US Pacific Northwest estuaries its areal coverage now exceeds that of the native eelgrass species, Zostera marina. Natural resource management’s...

  11. Expansion of the invasive dwarf eelgrass, Zostera japonica, in Yaquina Bay, Oregon

    Science.gov (United States)

    The areal coverage of the non-indigenous dwarf eelgrass, Zostera japonica, is increasing in several estuaries on the US West Coast. As a result, regulatory agencies in the states of California and Washington are considering methods of controlling its expansion. Factors relevan...

  12. Plastid structure and carotenogenic gene expression in red- and white-fleshed loquat (Eriobotrya japonica) fruits

    NARCIS (Netherlands)

    Fu, X.; Kong, W.; Peng, G.; Zhou, J.; Azam, M.; Xu, C.; Grierson, D.; Chen, K.

    2012-01-01

    Loquat (Eriobotrya japonica Lindl.) can be sorted into red- and white-fleshed cultivars. The flesh of Luoyangqing (LYQ, red-fleshed) appears red-orange because of a high content of carotenoids while the flesh of Baisha (BS, white-fleshed) appears ivory white due to a lack of carotenoid accumulation.

  13. Two cases of schistosomiasis japonica diagnosed on computed tomography and ultrasonography

    Energy Technology Data Exchange (ETDEWEB)

    Seki, Tatsuo; Midorikawa, Shigeo; Hagiri, Masumi; Itoh, Haruhisa; Haku, Eijitsu (Teikyo Univ., Tokyo (Japan). Faculty of Medicine)

    1982-12-01

    We reported two cases of schistosomiasis japonica which showed pathognomonic pattern on liver C.T. and ultrasonography. Schistosomiasis japonica is an endemic disease in areas of Japan, but only chronic case of the disease is found now since Schistosoma Japonicum is thought to be died out in Japan. The adult worms are most commonly in branches of portal system, such as superior mesenteric vein, and many eggs are carried to the liver, where they cause inflammatory pseudotubercle formation, periportal fibrosis, and calcifications in interlobular connective tissue. According to Nakayama et al, pathognomonic pattern of schistosomiasis japonica on U.S. for the liver consists of the amounts of the eggs' calcifications, that is, the more eggs' calcifications, the more pathognomonic U.S. finding. Typical sonographic finding in the liver is network or fishscale pattern, which consists of streaky high level echo and internal low level echo. C.T. similarly demonstrates mosaic high density streak resulting from eggs' calcifications in peripheral portal branches. In addition, if patient's past history shows the life in the endemic area such as these two cases, we can easily make a diagnosis of ''schistosomiasis japonica''.

  14. Two cases of schistosomiasis japonica diagnosed on computed tomography and ultrasonography

    International Nuclear Information System (INIS)

    Seki, Tatsuo; Midorikawa, Shigeo; Hagiri, Masumi; Itoh, Haruhisa; Haku, Eijitsu

    1982-01-01

    We reported two cases of schistosomiasis japonica which showed pathognomonic pattern on liver C.T. and ultrasonography. Schistosomiasis japonica is an endemic disease in areas of Japan, but only chronic case of the disease is found now since Schistosoma Japonicum is thought to be died out in Japan. The adult worms are most commonly in branches of portal system, such as superior mesenteric vein, and many eggs are carried to the liver, where they cause inflammatory pseudotubercle formation, periportal fibrosis, and calcifications in interlobular connective tissue. According to Nakayama et al, pathognomonic pattern of schistosomiasis japonica on U.S. for the liver consists of the amounts of the eggs' calcifications, that is, the more eggs' calcifications, the more pathognomonic U.S. finding. Typical sonographic finding in the liver is network or fishscale pattern, which consists of streaky high level echo and internal low level echo. C.T. similarly demonstrates mosaic high density streak resulting from eggs' calcifications in peripheral portal branches. In addition, if patient's past history shows the life in the endemic area such as ours two cases, we can easily make a diagnosis of ''schistosomiasis japonica''

  15. Bioactivity-guided isolation of antioxidant triterpenoids from Betula platyphylla var. japonica bark.

    Science.gov (United States)

    Eom, Hee Jeong; Kang, Hee Rae; Kim, Ho Kyong; Jung, Eun Bee; Park, Hyun Bong; Kang, Ki Sung; Kim, Ki Hyun

    2016-06-01

    The bark of Betula platyphylla var. japonica (Betulaceae) has been used to treat pneumonia, choloplania, nephritis, and chronic bronchitis. This study aimed to investigate the bioactive chemical constituents of the bark of B. platyphylla var. japonica. A bioassay-guided fractionation and chemical investigation of the bark of B. platyphylla var. japonica resulted in the isolation and identification of a new lupane-type triterpene, 27-hydroxybetunolic acid (1), along with 18 known triterpenoids (2-19). The structure of the new compound (1) was elucidated on the basis of 1D and 2D NMR spectroscopic data analysis as well as HR-ESIMS. Among the known compounds, chilianthin B (17), chilianthin C (18), and chilianthin A (19) were triterpene-lignan esters, which are rarely found in nature. Compounds 4, 6, 7, 17, 18, and 19 showed significant antioxidant activities with IC50 values in the range 4.48-43.02μM in a DPPH radical-scavenging assay. However, no compound showed significant inhibition of acetylcholine esterase (AChE). Unfortunately, the new compound (1) exhibited no significance in both biological activities. This study strongly suggests that B. platyphylla var. japonica bark is a potential source of natural antioxidants for use in pharmaceuticals and functional foods. Copyright © 2016 Elsevier Inc. All rights reserved.

  16. Characterization and analysis of a de novo transcriptome from the pygmy grasshopper Tetrix japonica.

    Science.gov (United States)

    Qiu, Zhongying; Liu, Fei; Lu, Huimeng; Huang, Yuan

    2017-05-01

    The pygmy grasshopper Tetrix japonica is a common insect distributed throughout the world, and it has the potential for use in studies of body colour polymorphism, genomics and the biology of Tetrigoidea (Insecta: Orthoptera). However, limited biological information is available for this insect. Here, we conducted a de novo transcriptome study of adult and larval T. japonica to provide a better understanding of its gene expression and develop genomic resources for future work. We sequenced and explored the characteristics of the de novo transcriptome of T. japonica using Illumina HiSeq 2000 platform. A total of 107 608 206 paired-end clean reads were assembled into 61 141 unigenes using the trinity software; the mean unigene size was 771 bp, and the N50 length was 1238 bp. A total of 29 225 unigenes were functionally annotated to the NCBI nonredundant protein sequences (Nr), NCBI nonredundant nucleotide sequences (Nt), a manually annotated and reviewed protein sequence database (Swiss-Prot), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. A large number of putative genes that are potentially involved in pigment pathways, juvenile hormone (JH) metabolism and signalling pathways were identified in the T. japonica transcriptome. Additionally, 165 769 and 156 796 putative single nucleotide polymorphisms occurred in the adult and larvae transcriptomes, respectively, and a total of 3162 simple sequence repeats were detected in this assembly. This comprehensive transcriptomic data for T. japonica will provide a usable resource for gene predictions, signalling pathway investigations and molecular marker development for this species and other pygmy grasshoppers. © 2016 John Wiley & Sons Ltd.

  17. The Soil-Root Strength Performance of Alternanthera Ficoidea and Zoysia Japonica as Green Roof Vegetation

    Directory of Open Access Journals (Sweden)

    Abdullah Muhamad Firdaurs

    2017-01-01

    Full Text Available The rise of awareness on environmentalism has demanded that all parties involved in built environment to implement green technology in their construction projects. Great care must be taken when designing a green roof system including the selection of plants and appropriate substrates. This study was performed to investigate the soil-root composite strength of two types of green roof vegetation (A. Ficoidea and Z. Japonica at different growth periods for up to 6 months. Both plants were planted in six plastic plots (45 cm × 29 cm × 13 cm containing a mixture of perlite, vermiculite and organic soil. Every two months, a series of direct shear tests were conducted on a sample from each species to determine the root-soil shear strength. The tests continued until the 6th month. The average results showed that Z. Japonica had higher soil-root shear strength (49.1 kPa compared to A. Ficoidea after two months of growth. In the 4th month however, A. Ficoidea managed to surpass Z. Japonica (28.7 kPa versus 18.5 kPa in terms of shear strength. However, their average peak shear strength decreased sharply compared to the previous month. Lastly, in six months, A. Ficoidea sustained a higher average peak soil shear strength (56.5 kPa compared to Z. Japonica (14.3 kPa. Therefore, it can be concluded that A. Ficoidea may offer a better soil reinforcement than Z. japonica and thus it could potentially be a good choice of green roof vegetation.

  18. Lineage diversification and hybridization in the Cayratia japonica-Cayratia tenuifolia species complex.

    Science.gov (United States)

    Ishikawa, Naoko; Ikeda, Hajime; Yi, Ting-shuang; Takabe-Ito, Eriko; Okada, Hiroshi; Tsukaya, Hirokazu

    2014-06-01

    The Cayratia japonica-Cayratia tenuifolia species complex (Vitaceae) is distributed from temperate to tropical East Asia, Southeast Asia, India, and Australia. The spatiotemporal diversification history of this complex was assessed through phylogenetic and biogeographic analyses. Maximum parsimony, neighbor-joining, and maximum likelihood methods were used to analyze sequences of one nuclear (AS1) and two plastid regions (trnL-F and trnC-petN). Bayesian dating analysis was conducted to estimate the divergence times of clades. The likelihood method LAGRANGE was used to infer ancestral areas. The Asian C. japonica and C. tenuifolia should be treated as an unresolved complex, and Australian C. japonica is distinct from the Asian C. japonica-C. tenuifolia species complex and should be treated as separate taxa. The Asian C. japonica-C. tenuifolia species complex was estimated to have diverged from its closest relatives during the Late Eocene (35.1 million years ago [Ma], 95% highest posterior densities [HPD]=23.3-47.3Ma) and most likely first diverged in mid-continental Asia. This complex was first divided into a northern clade and a southern clade during the middle Oligocene (27.3Ma; 95% HPD=17.4-38.1Ma), which is consistent with a large southeastward extrusion of the Indochina region relative to South China along the Red River. Each of the northern and southern clades then further diverged into multiple subclades through a series of dispersal and divergence events following significant geological and climatic changes in East and Southeast Asia during the Miocene. Multiple inter-lineage hybridizations among four lineages were inferred to have occurred following this diversification process, which caused some Asian lineages to be morphologically cryptic. Copyright © 2014 Elsevier Inc. All rights reserved.

  19. Factores de riesgo de bajo peso al nacer

    Directory of Open Access Journals (Sweden)

    Marcelo Bortman

    1998-05-01

    Full Text Available El bajo peso al nacer (BPN es el principal factor determinante conocido de la mortalidad infantil. Pese a la notable reducción de las tasas de mortalidad infantil y al aumento de las tasas de supervivencia de niños con BPN, en Neuquén, Argentina, no se han observado descensos notables de las tasas de BPN. El propósito de este estudio fue conocer los factores de riesgo de BPN, su frecuencia en la población y el papel de la atención prenatal en su prevención, y desarrollar una escala de riesgo que permita identificar a las mujeres en mayor riesgo de dar a luz un niño de bajo peso. Con tal propósito se realizó un estudio transversal en el cual se utilizó 50% de los datos del Sistema Informático Perinatal correspondientes al período 1988­1995 y procedentes de los 29 hospitales de la provincia de Neuquén (46 171 nacimientos. Se analizaron la distribución del peso y la frecuencia de los posibles factores de riesgo de BPN. La asociación entre estos factores y el BPN se analizó mediante un modelo de regresión logística. Con los resultados obtenidos se desarrolló una escala aditiva, que fue validada con el 50% restante de los registros de nacimientos. La razón de posibilidades (RP más elevada correspondió a la falta de atención prenatal (RP = 8,78; IC95%: 6,7 a 11,4. Las RP asociadas con una atención prenatal inadecuada, una primera consulta prenatal tardía, preeclampsia o eclampsia, hemorragia o anomalías de la placenta o sus membranas e historia de un hijo previo con BPN fueron mayores de 2,0. El riesgo de tener hijos con BPN también fue más alto en las mujeres mayores de 40 años, las menores de 20, las solteras, las fumadoras, aquellas cuyo intervalo intergenésico fue menor de 18 meses y las que tenían un índice de masa corporal menor de 20. Por último, se encontró una relación lineal directa entre el puntaje en la escala y el riesgo de tener un hijo con BPN.

  20. Effects of Temperature, Salinity and Seed Age on Induction of Zostera japonica Germination in North America, USA

    Science.gov (United States)

    Seagrasses can colonize unstructured mudflats either through clonal growth or seed germination and survival. Zostera japonica is an introduced seagrass in North America that has rapidly colonized mudflats along the Pacific Coast, leading to active management of the species. Gro...

  1. Mapping estuarine distributions of the non-indigenous Japanese Eelgrass Zostera japonica using Color Infrared Aerial Photography

    Science.gov (United States)

    This presentation describes a technique for mapping distributions of the nonindigenous Japanese eelgrass Zostera japonica in estuarine ecosystems of the Pacific Northwest. The relatively broad distribution of this intertidal plant, often on very soft substrate, makes classical g...

  2. Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress

    KAUST Repository

    Yun, Kil-Young; Park, Myoung Ryoul; Mohanty, Bijayalaxmi; Herath, Venura; Xu, Fuyu; Mauleon, Ramil; Wijaya, Edward; Bajic, Vladimir B.; Bruskiewich, Richard; de los Reyes, Benildo G

    2010-01-01

    -plant level analyses established a holistic view of chilling stress response mechanism of japonica rice. Early response regulatory network triggered by oxidative signals is critical for prolonged survival under sub-optimal temperature. Integration of stress

  3. ¿Aerolíneas de bajo costo en colombia?

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Mantilla

    2003-11-01

    Full Text Available Con la apertura del mercado en Colombia, la competencia entre aerolíneas se agudizó ante la entrada de nuevos operadores y la flexibilización de tarifas como estrategia para la captación de una mayor demanda. Las ventajas competitivas de cada aerolínea fueron el punto clave para la disputa del mercado y todas ellas trabajan hoy en fórmulas que les permitan maximizar beneficios y minimizar costos, enfrentando así la crisis que vive la industria, debido a los acontecimientos del 11 de septiembre de 2001. Puede decirse que los grandes esfuerzos se hacen en los departamentos comerciales, pues debido al auge de las tecnologías y al uso del internet, tanto empresas de transporte aéreo como agentes turísticos deben enfrentar el problema de la competencia de precios. Sin embargo, esto les ofrece a las aerolíneas la oportunidad de comercializar sus servicios a "bajo costo", esquema que ha resultado favorable para empresas y clientes.

  4. Factores de riesgo del bajo peso al nacer

    Directory of Open Access Journals (Sweden)

    Ernesto Rosell Juarte

    1996-06-01

    Full Text Available Se realizó una investigación de casos y controles en las mujeres que parieron niños con bajo peso, de los consultorios urbanos de médicos de la familia pertenecientes al Policlínico Comunitario Docente "Carlos J. Finlay" de Camagüey, durante el año 1994, con el objetivo de identificar los factores de riesgo biológico y socioeconómicos que con más frecuencia se asociaron; se estudiaron 32 "casos" y 53 "controles" de un universo de 521 nacidos vivos. Se demostró que la edad materna hasta 19 años y de 35 y más, la ganancia insuficiente de peso durante la gestación, el antecedente familiar de prematuridad, la amenaza de parto pretérmino y la ruptura prematura de las membranas, resultaron ser factores de riesgo, y esto no coincide con lo reportado por algunos autores en la evaluación de otros factores asociados.

  5. Consumption of Bt Maize Pollen Containing Cry1Ie Does Not Negatively Affect Propylea japonica (Thunberg (Coleoptera: Coccinellidae

    Directory of Open Access Journals (Sweden)

    Yonghui Li

    2017-03-01

    Full Text Available Propylea japonica (Thunberg (Coleoptera: Coccinellidae are prevalent predators and pollen feeders in East Asian maize fields. They are therefore indirectly (via prey and directly (via pollen exposed to Cry proteins within Bt-transgenic maize fields. The effects of Cry1Ie-producing transgenic maize pollen on the fitness of P. japonica was assessed using two dietary-exposure experiments in the laboratory. In the first experiment, survival, larval developmental time, adult fresh weight, and fecundity did not differ between ladybirds consuming Bt or non-Bt maize pollen. In the second experiment, none of the tested lethal and sublethal parameters of P. japonica were negatively affected when fed a rapeseed pollen-based diet containing Cry1Ie protein at 200 μg/g dry weight of diet. In contrast, the larval developmental time, adult fresh weight, and fecundity of P. japonica were significantly adversely affected when fed diet containing the positive control compound E-64. In both experiments, the bioactivity of the Cry1Ie protein in the food sources was confirmed by bioassays with a Cry1Ie-sensitive lepidopteran species. These results indicated that P. japonica are not affected by the consumption of Cry1Ie-expressing maize pollen and are not sensitive to the Cry1Ie protein, suggesting that the growing of Bt maize expressing Cry1Ie protein will pose a negligible risk to P. japonica.

  6. Anti-oxidative feedback and biomarkers in the intertidal seagrass Zostera japonica induced by exposure to copper, lead and cadmium

    International Nuclear Information System (INIS)

    Lin, Haiying; Sun, Tao; Zhou, Yi; Zhang, Xiaomei

    2016-01-01

    To investigate the potential influences of anthropogenic pollutants, we evaluated the responses of the intertidal seagrass Zostera japonica to three heavy metals: copper (Cu), lead (Pb), and cadmium (Cd). Z. japonica was exposed to various concentrations of Cu, Pb , and Cd (0, 0.5, 5, 50 μM) over seven days. The effects were then analyzed using the antioxidant enzyme catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione peroxidase (GPX), and lipid peroxidation measured using malondialdehyde (MDA) as proxy. Metal accumulation in the above-ground tissues and phenotypic changes were also investigated. Our results revealed that heavy metal concentration increased in seagrass exposed to high levels of metals. Z. japonica has great potential for metal accumulation and a suitable candidate for the decontamination of moderately Cu contaminated bodies of water and can also potentially enhanced efforts of environmental decontamination, either through phytoextraction abilities or by functioning as an indicator for monitoring programs that use SOD, CAT, GPX, POD and MDA as biomarkers. - Highlights: • Anti-oxidative feedback of Zostera japonica to the heavy metals Cu, Pb, and Cd was determined. • The endangered intertidal seagrass Z. japonica had a high metal accumulation potential. • Z. japonica might be a potential indicator in monitoring programs using SOD, CAT, GPX, POD and MDA as biomarkers.

  7. Effect of Biochar on Relieving Cadmium Stress and Reducing Accumulation in Super japonica Rice

    Institute of Scientific and Technical Information of China (English)

    ZHANG Zhen-yu; MENG Jun; DANG Shu; CHEN Wen-fu

    2014-01-01

    It is of great importance to solve the threats induced by cadmium pollution on crops. This paper examined the effect of biochar on cadmium accumulation in japonica rice and revealed the mechanism underlying the response of protective enzyme system to cadmium stress. Biochar derived from rice straw was applied at two application rates under three cadmium concentrations. Shennong 265, super japonica rice variety, was selected as the test crop. The results indicated that cadmium content in above-ground biomass of rice increased with increasing soil cadmium concentrations, but the biochar application could suppress the accumulation of cadmium to some extent. Under high concentrations of cadmium, content of free proline and MDA (malondialdehyde) were high, so did the SOD (superoxide dismutase), POD (peroxidase) and CAT (catalase) activity in the lfag leaf of rice. However, the protective enzyme activities remained at low level when biochar was added.

  8. Antimicrobial evaluation of the crude extract of symbiotic fungi from marine sponge Reniera japonica

    Directory of Open Access Journals (Sweden)

    Xuelian Bai

    2018-05-01

    Full Text Available Marine sponge-derived microbes are one of the rich sources of bioactive natural products with a broad spectrum of bioactivities. The present work focuses on the isolation and antimicrobial screening of the marine sponge-associated fungi from Reniera japonica MNP-2016. The results indicated that five fungi (L1-2, L2-1, L4, L8-1 and L14 were successfully isolated. Bioassay tests showed that only strain L14 had strong inhibitory effect on the pathogens, Staphyloccocus aureus, Escherichia coli and Candida albicans. 18S rDNA sequence analysis indicated that strain L14 was ascribed to Aspergillus genus. To the best of our knowledge, this work was the first report on the isolation and antimicrobial evaluation of fungi from R. japonica.

  9. Complete genome sequence of the actinobacterium Amycolatopsis japonica MG417-CF17T (=DSM 44213T) producing (S,S)-N,N′-ethylenediaminedisuccinic acid

    DEFF Research Database (Denmark)

    Stegmann, Evi; Albersmeier, Andreas; Spohn, Marius

    2014-01-01

    We report the complete genome sequence of Amycolatopsis japonica MG417-CF17T (=DSM 44213T) which was identified as the producer of (S,S)-N,N′-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17T consists of two replicons: the chro......We report the complete genome sequence of Amycolatopsis japonica MG417-CF17T (=DSM 44213T) which was identified as the producer of (S,S)-N,N′-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17T consists of two replicons...

  10. Ley marco de aseguramiento universal en salud, bajo la lupa

    Directory of Open Access Journals (Sweden)

    Leoncio Díaz

    2009-04-01

    Full Text Available La Ley Marco de Aseguramiento Universal en Salud, concebida en el marco del modelo neoliberal conservador, excluyente y polarizante, fue aprobada en circunstancias en la que persisten grandes contrastes entre sistemas de salud. Por un lado, los países que mantienen subsistemas inconexos y privatizados y, por otro, los que unificados y bajo la responsabilidad del Estado, garantizan a todos el acceso integral, gratuito y de calidad a los servicios de salud. En América y el Caribe, existen países cuyos pobladores carecen de derechos y posibilidades, evidenciando las profundas inequidades que mantienen en exclusión a la mayoría pobre y de extrema pobreza, entre ellos, el Perú muestra indicadores de salud que aún se mantienen en cifras alarmantes y por debajo de los que presentan otras realidades en el mundo. En las últimas tres décadas hubo ensayos frustros de reformas, como la integración funcional del Ministerio de Salud con el Instituto Peruano de Seguridad Social, o el Sistema Nacional Coordinado y Descentralizado en Salud. Actualmente se formula la Ley Marco de Aseguramiento Universal, que mantiene los actuales subsistemas sin variación alguna y con sus propias autonomías, ensayando pilotos, estableciendo planes parciales, implementándola de manera progresiva, priorizando la privatización y debilitando la función rectora del Ministerio de Salud, al crear una superintendencia omnipotente, lo cual continuará permitiendo la exclusión social de más de 9 millones de peruanos. Existe, por tanto, la necesidad de plantear la unificación de los subsistemas, para construir un Sistema Único de Salud, capaz de otorgar salud plena, integral, de calidad y sin costo alguno al 100% de ciudadanos.

  11. Capital intelectual bajo el modelo de e-commerce

    Directory of Open Access Journals (Sweden)

    Gladys Rodríguez

    2009-09-01

    Full Text Available Hoy el mundo está cambiando de una economía basada en el acero, automóviles y carreteras hacia una nueva economía construida con base en silicio, computadoras y redes. Esta revolución tecnológica no se limita a modificar los equipos, los productos, las trayectorias tecnológicas y el modelo organizativo. Sino, como en todo cambio de paradigma hay un proceso de desmantelamiento de lo establecido y la construcción de un nuevo conjunto de principios. Hoy ese nuevo paradigma se fundamenta en el conocimiento, la pericia y sobre todo el talento del hombre. Por tanto, el objetivo principal es describir la importancia que tiene el capital intelectual en las organizaciones de e-commerce. La metodología empleada consistió en una revisión teórica con base a la literatura especializada de autores tales como: Edvinsson y Malone (1998, Brooking (1997, Luz Clara (2001, Merino (2004 y Añez (2005: orientándose hacia el capital intelectual bajo el esquema de negocios de organizaciones de e-commerce. Entre los resultados se destacan dos propuestas: una que describe el proceso de transformación de un negocio tradicional a un negocio de e-commerce sobre la base del capital intelectual; la otra, referida a indicar estrategias gerenciales que apuntan a identificar y evaluar la relación del capital intelectual en cuanto a las innovaciones/ respuestas a los cambios exigidos por el proceso de globalización en el marco de organizaciones de e-commerce.

  12. Japanese beetle Popillia japonica Newman : foliar feeding on wine grapes in Virginia

    OpenAIRE

    Boucher, T. Jude

    1986-01-01

    The natural infestation level for 1985 of the Japanese beetle, Popillia japonica Newman, in the Shenandoah Valley of Virginia failed to reduce berry quality, yield or shoot growth in a commercial vineyard. Intensive postveraison foliage feeding by Japanese beetle resulted 1n fruit with lower soluble solids and higher total titratable acidity at harvest, but did not affect pH, sugar per berry, berry weight, yield, leaves per vine or shoot length. Intensive previraison feeding a...

  13. Evolution and Expansion of the Prokaryote-Like Lipoxygenase Family in the Brown Alga Saccharina japonica

    Directory of Open Access Journals (Sweden)

    Linhong Teng

    2017-11-01

    Full Text Available Lipoxygenase (LOX plays important roles in fatty acid oxidation and lipid mediator biosynthesis. In this study, we give first insights into brown algal LOX evolution. Whole genome searches revealed four, three, and eleven LOXs in Ectocarpus siliculosus, Cladosiphon okamuranus, and Saccharina japonica, respectively. In phylogenetic analyses, LOXs from brown algae form a robust clade with those from prokaryotes, suggesting an ancestral origin and slow evolution. Brown algal LOXs were divided into two clades, C1 and C2 in a phylogenetic tree. Compared to the two species of Ectocarpales, LOX gene expansion occurred in the kelp S. japonica through tandem duplication and segmental duplication. Selection pressure analysis showed that LOX genes in brown algae have undergone strong purifying selection, while the selective constraint in the C2 clade was more relaxed than that in the C1 clade. Furthermore, within each clade, LOXs of S. japonica evolved under more relaxed selection constraints than E. siliculosus and C. okamuranus. Structural modeling showed that unlike LOXs of plants and animals, which contain a β barrel in the N-terminal part of the protein, LOXs in brown algae fold into a single domain. Analysis of previously published transcriptomic data showed that LOXs in E. siliculosus are responsive to hyposaline, hypersaline, oxidative, and copper stresses. Moreover, clear divergence of expression patterns was observed among different life stages, as well as between duplicate gene pairs. In E. siliculosus, all four LOXs are male-biased in immature gametophytes, and mature gametophytes showed significantly higher LOX mRNA levels than immature gametophytes and sporophytes. In S. japonica, however, our RNA-Seq data showed that most LOXs are highly expressed in sporophytes. Even the most recently duplicated gene pairs showed divergent expression patterns, suggesting that functional divergence has likely occurred since LOX genes duplicated, which

  14. Distribution of Tributyltin in Tissues of Mature Japanese Whiting, Sillago japonica and Their Eggs

    OpenAIRE

    Shimasaki, Yohei; Oshima, Yuji; Inoue, Yoshiyuki; Shibata, Hisashi; Nakayama, Kei; Inoue, Suguru; Imoto, Hisaya; Kang, Ik Joon; Honjo, Tsuneo

    2008-01-01

    Tributyltin (TBT) has continued to pollute the coastal areas therein following global regulation for its use as an anti-fouling agent. The tissue dynamics of TBT in fish have been extensively documented, but few studies on maternal transfer of TBT have been performed. Previously, we reported that TBT was maternally transferred from parent fish to eggs. The present study examined the distribution of TBT in the tissues and spawned eggs of Japanese whiting, Sillago japonica, after dietary exp...

  15. Effect of Slave Raiding of Polyergus samurai on Nest Persistency of Its Host, Formica (Serviformica) japonica

    OpenAIRE

    Hasegawa, Eisuke; Yamaguchi, Takeshi

    1997-01-01

    The effect of slave raiding of Polyergus samurai on nest persistency of its host, Formica (Serviformica) japonica was investigated. Nest persistence rate after the raiding season was not different between raided and unraided nests. Many raided nests stopped aboveground activities for 2-3 weeks after a raiding but surviving workers maintained underground nest structure. These observations suggest that it is required to reconsider the previous interpretation that the raided nests died out from ...

  16. Gonadal differentiation in frogs, Rana japonica and R. brevipoda, raised from UV irradiated eggs

    International Nuclear Information System (INIS)

    Shirane, T.

    1982-01-01

    The gonadal differentiation of anurans, Rana japonica and R. brevipoda, was examined in animals raised from eggs which had been irradiated at the vegetal hemisphere with UV (9300 erg/mm2) at the 2-cell stage. In R. japonica about 70% of the larvae at stage I from the pressed and UV-irradiated eggs were germ cell free, but at a stage immediately after metamorphosis all animals had at least some germ cells, although their gonads often were extremely small and poorly differentiated. When male animals matured sexually, many of them had abnormal gonads. However, all of them were shown by artificial means to be capable of fertilization. In the nonpressed and irradiated group, no larvae were germ cell free and the animals immediately after metamorphosis showed nearly normal gonadal differentiation except for the presence of a few degenerate oocytes in the ovaries. The results in R. brevipoda were basically similar to those in R. japonica. In both species, sex ratios were determined at two stages, the first immediately after metamorphosis and the other when the animals matured, as based on gonad morphology and histology and on external sexually dimorphic characters as well. Sex ratios at these two stages in frogs from the pressed and irradiated eggs differed markedly in R. brevipoda. The ratio was normal at metamorphosis but high M/F ratios occurred when animals became mature. That sex reversal took place in this species as well as in R. japonica (in which sex-ratio deviation was not statistically significant) was supported by the sex ratios of the progenies of these supernumerary males

  17. Mapping of incidence and management of invasive species Fallopia japonica at selected locations of northwestern Slovakia

    International Nuclear Information System (INIS)

    Paukova, Z.; Krskova, L.

    2011-01-01

    Mapping of one of the most dangerous invasive species Fallopia japonica was conducted at selected locations of northwestern Slovakia: in the cadastral territory of Zazriva, in the village Parnica towards Kralovany, in the village Kralovany and the city of Dolny Kubin - Zaskalie, stretch Timravina in the Lower Orava in late summer and early autumn 2009. We recorded by field survey 24 invading Japanese knotweed in the area of 12.238 m 2 . (authors)

  18. Variation in Yield and Physicochemical Quality Traits among Mutants of Japonica Rice Cultivar Wuyujing 3

    OpenAIRE

    Abacar, Jose Daniel; Zhao-miao, Lin; Xin-cheng, Zhang; Cheng-qiang, Ding; She, Tang; Zheng-hui, Liu; Shao-hua, Wang; Yan-feng, Ding

    2016-01-01

    To select elite germplasms, 112 mutants derived from japonica rice cultivar Wuyujing 3 were evaluated. The yield components such as panicle number per square meter, grain number per panicle, and grain weight were measured. The quality traits such as percentage of chalky grains (PCG), brown rice yield (BRY), milled rice yield (MRY), degree of milling (DM), amylose content (AC), protein content (PC), and relationships among traits were inverstigated. Results showed that grain yield ranged from ...

  19. Overcoming inter-subspecific hybrid sterility in rice by developing indica-compatible japonica lines.

    Science.gov (United States)

    Guo, Jie; Xu, Xiaomei; Li, Wentao; Zhu, Wenyin; Zhu, Haitao; Liu, Ziqiang; Luan, Xin; Dai, Ziju; Liu, Guifu; Zhang, Zemin; Zeng, Ruizhen; Tang, Guang; Fu, Xuelin; Wang, Shaokui; Zhang, Guiquan

    2016-06-01

    Rice (Oryza sativa L.) is an important staple crop. The exploitation of the great heterosis that exists in the inter-subspecific crosses between the indica and japonica rice has long been considered as a promising way to increase the yield potential. However, the male and female sterility frequently occurred in the inter-subspecific hybrids hampered the utilization of the heterosis. Here we report that the inter-subspecific hybrid sterility in rice is mainly affected by the genes at Sb, Sc, Sd and Se loci for F1 male sterility and the gene at S5 locus for F1 female sterility. The indica-compatible japonica lines (ICJLs) developed by pyramiding the indica allele (S-i) at Sb, Sc, Sd and Se loci and the neutral allele (S-n) at S5 locus in japonica genetic background through marker-assisted selection are compatible with indica rice in pollen fertility and in spikelet fertility. These results showed a great promise of overcoming the inter-subspecific hybrid sterility and exploiting the heterosis by developing ICJLs.

  20. The formation of pollen in male flowers and yearly atmospheric pollen counts of Cryptomeria japonica in the following year

    Directory of Open Access Journals (Sweden)

    Hideaki Taira

    1998-01-01

    Full Text Available Prediction of yearly atmospheric pollen counts is a very important component in the prevention of allergenic symptoms. We investigated the relationship between atomspheric pollen counts and the formation of male flowers of Cryptomeriajaponica D. Don (C. japonica. An atmospheric pollen survey of C. japonica was conducted from 1983 to 1996 using a Durham’s sampler. A regression analysis was performed between the total pollen count and July temperature in previous years. The atmospheric pollen counts of C. japonica had a high positive correlation with the mean temperature in July of the previous year. However, the predicted using average mean July temperature records of the previous year were insufficient, especially in years following high pollen count. In experimental conditions, using 60 C. japonica trees in pots, the formation of male flowers was shown to increase with a rise in incubation temperature. In a forest of C. japonica, our results showed that the length and weight of new needle growth from old needles, which produced many flowers in the previous year, were shorter and lighter, respectively. These aerobiological and plant physiological studies provide evidence that a smaller number of pollen counts are a common result in a year following one in which many male flowers are produced, even if the mean July temperature of that year was high.

  1. Comprehensive evaluation of candidate reference genes for gene expression studies in Lysiphlebia japonica (Hymenoptera: Aphidiidae) using RT-qPCR.

    Science.gov (United States)

    Gao, Xue-Ke; Zhang, Shuai; Luo, Jun-Yu; Wang, Chun-Yi; Lü, Li-Min; Zhang, Li-Juan; Zhu, Xiang-Zhen; Wang, Li; Lu, Hui; Cui, Jin-Jie

    2017-12-30

    Lysiphlebia japonica (Ashmead) is a predominant parasitoid of cotton-melon aphids in the fields of northern China with a proven ability to effectively control cotton aphid populations in early summer. For accurate normalization of gene expression in L. japonica using quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR), reference genes with stable gene expression patterns are essential. However, no appropriate reference genes is L. japonica have been investigated to date. In the present study, 12 selected housekeeping genes from L. japonica were cloned. We evaluated the stability of these genes under various experimental treatments by RT-qPCR using four independent (geNorm, NormFinder, BestKeeper and Delta Ct) and one comparative (RefFinder) algorithm. We identified genes showing the most stable levels of expression: DIMT, 18S rRNA, and RPL13 during different stages; AK, RPL13, and TBP among sexes; EF1A, PPI, and RPL27 in different tissues, and EF1A, RPL13, and PPI in adults fed on different diets. Moreover, the expression profile of a target gene (odorant receptor 1, OR1) studied during the developmental stages confirms the reliability of the chosen selected reference genes. This study provides for the first time a comprehensive list of suitable reference genes for gene expression studies in L. japonica and will benefit subsequent genomics and functional genomics research on this natural enemy. Copyright © 2017. Published by Elsevier B.V.

  2. A tale of two seagrasses: Comparing the science and management of Zostera marina and Zostera japonica in the Pacific Northwest - CERF

    Science.gov (United States)

    On the Pacific coast of North America, at least two congeners of Zostera occur: native Z. marina, and introduced, Z. japonica. Z. marina is protected by State and Federal laws as essential fish habitat. Z. japonica is considered “invasive” and therefore, ecologicall...

  3. COMPARISON OF ANNUAL PRODUCTION ECOLOGY OF NATIVE EELGRASS ZOSTERA MARINA AND THE NON-NATIVE DWARF EELGRASS Z. JAPONICA IN YAQUINA BAY, OREGON

    Science.gov (United States)

    When non-native plant species invade a system they often change patterns of primary production. I evaluate the contribution of the seagrass Zostera marina and it's non-native congener Z. japonica to primary production in Yaquina Bay. Few measurements of Z. japonica production e...

  4. Sexual Differences in Chemical Composition and Aroma-active Compounds of Essential Oil from Flower Buds of Eurya japonica.

    Science.gov (United States)

    Miyazawa, Mitsuo; Usami, Atsushi; Tanaka, Takio; Tsuji, Kaoru; Takehara, Manami; Hori, Yuki

    2016-01-01

    This study was conducted to determine the composition of essential oil from buds of male and female Eurya japonica flowers and to determine the aroma-active compounds of this plant by gas chromatography-mass spectrometry (GC-MS), sensory evaluation, and odor activity values (OAV). The oils contained eighty-five compounds. We identified for the first time forty-four compounds in E. japonica. Through sensory evaluation, nineteen aroma-active compounds were identified by gas chromatography-olfactometry (GC-O). Because the chemical composition can affect the interaction between plants and herbivorous insects, our results suggest that essential oils from male and female flower buds of E. japonica differently affect herbivores. Sexual differences in essential oils deserve further investigations in this plant-insect system.

  5. El crecimiento y desarrollo ante la prematuridad y el bajo peso al nacer

    Directory of Open Access Journals (Sweden)

    Christine Baccarat de Godoy Martins

    2015-09-01

    Full Text Available Objetivo: Realizar una revisión bibliográfica sobre el crecimiento y desarrollo de prematuros y/o de bajo peso al nacer. Metodología: Se utilizaron las bases de datos lilacs, medline y scielo. Se encontraron 64 trabajos: 10 publicados en 2005, 10 en 2006, 12 en 2007, 16 en 2008, 8 en 2009 y 8 en 2010. Las publicaciones halladas pertenecían a las áreas de medicina (59,4%, nutrición (7,8%, enfermería (7,8%, salud pública (7,8%, psicología (6,2%, epidemiología (4,7%, fisiología (3,1% y fonoaudiología (3,1%. Resultados:Los textos encontrados permitieron la organización del conocimiento producido según las siguientes categorías de análisis: Factores asociados al bajo peso en el nacimiento/prematuridad; Repercusiones del bajo peso en el nacimiento/prematuridad para el crecimiento y desarrollo y medidas de prevención; y Supervivencia de los recién nacidos prematuros o de bajo peso. Conclusión: Los niños nacidos prematuramente y de bajo peso generalmente presentan déficit de desarrollo. Un acompañamiento postnatal centrado en el crecimiento y desarrollo es fundamental.

  6. Fallopia japonica, a Natural Modulator, Can Overcome Multidrug Resistance in Cancer Cells

    Directory of Open Access Journals (Sweden)

    Safaa Yehia Eid

    2015-01-01

    Full Text Available Resistance of cancer cells to chemotherapy is controlled by the decrease of intracellular drug accumulation, increase of detoxification, and diminished propensity of cancer cells to undergo apoptosis. ATP-binding cassette (ABC membrane transporters with intracellular metabolic enzymes contribute to the complex and unresolved phenomenon of multidrug resistance (MDR. Natural products as alternative medicine have great potential to discover new MDR inhibitors with diverse modes of action. In this study, we characterized several extracts of traditional Chinese medicine (TCM plants (N = 16 for their interaction with ABC transporters, cytochrome P3A4 (CYP3A4, and glutathione-S-transferase (GST activities and their cytotoxic effect on different cancer cell lines. Fallopia japonica (FJ (Polygonaceae shows potent inhibitory effect on CYP3A4 P-glycoprotein activity about 1.8-fold when compared to verapamil as positive control. FJ shows significant inhibitory effect (39.81% compared with the known inhibitor ketoconazole and 100 μg/mL inhibited GST activity to 14 μmol/min/mL. FJ shows moderate cytotoxicity in human Caco-2, HepG-2, and HeLa cell lines; IC50 values were 630.98, 198.80, and 317.37 µg/mL, respectively. LC-ESI-MS were used to identify and quantify the most abundant compounds, emodin, polydatin, and resveratrol, in the most active extract of FJ. Here, we present the prospect of using Fallopia japonica as natural products to modulate the function of ABC drug transporters. We are conducting future study to evaluate the ability of the major active secondary metabolites of Fallopia japonica to modulate MDR and their impact in case of failure of chemotherapy.

  7. Effect of gamma irradiation on Agrobacterium-mediated genetic transformation of Japanese lawngrass (Zoysia japonica Steud.)

    International Nuclear Information System (INIS)

    Zhang Lei; Anhui Agricultural Univ., Hefei; Hu Fanrong; Zhang Linlin; Wang Xueyan; Wu Dianxing; Ma Chuanxi

    2004-01-01

    The effects of gamma irradiation on Agrobacterium-mediated genetic transformation were investigated in the current paper, using embryonic calli derived from the mature seeds of Japanese lawngrass (Zoysia japonica Steud.). The result indicated that the GUS transient expression rates were enhanced with the increasing doses when treated by doses lower than 4 Gy, however it would be decreased when treated by doses higher than 4 Gy. Based on the survival rate and GUS transient expression rate, 2 Gy is the optimal dose for Agrobacterium-mediated genetic transformation. Further observation found that 36 hours reculture after gamma irradiation is the most appropriate for agrobacterium infection. (authors)

  8. Quantifying quality of life and disability of patients with advanced schistosomiasis japonica.

    Directory of Open Access Journals (Sweden)

    Tie-Wu Jia

    Full Text Available BACKGROUND: The Chinese government lists advanced schistosomiasis as a leading healthcare priority due to its serious health and economic impacts, yet it has not been included in the estimates of schistosomiasis burden in the Global Burden of Disease (GBD study. Therefore, the quality of life and disability weight (DW for the advanced cases of schistosomiasis japonica have to be taken into account in the re-estimation of burden of disease due to schistosomiasis. METHODOLOGY/PRINCIPAL FINDINGS: A patient-based quality-of-life evaluation was performed for advanced schistosomiasis japonica. Suspected or officially registered advanced cases in a Schistosoma japonicum-hyperendemic county of the People's Republic of China (P.R. China were screened using a short questionnaire and physical examination. Disability and morbidity were assessed in confirmed cases, using the European quality of life questionnaire with an additional cognitive dimension (known as the "EQ-5D plus", ultrasonography, and laboratory testing. The age-specific DW of advanced schistosomiasis japonica was estimated based on patients' self-rated health scores on the visual analogue scale of the questionnaire. The relationships between health status, morbidity and DW were explored using multivariate regression models. Of 506 candidates, 215 cases were confirmed as advanced schistosomiasis japonica and evaluated. Most of the patients reported impairments in at least one health dimension, such as pain or discomfort (90.7%, usual activities (87.9%, and anxiety or depression (80.9%. The overall DW was 0.447, and age-specific DWs ranged from 0.378 among individuals aged 30-44 years to 0.510 among the elderly aged ≥ 60 years. DWs are positively associated with loss of work capacity, psychological abnormality, ascites, and active hepatitis B virus, while splenectomy and high albumin were protective factors for quality of life. CONCLUSIONS/SIGNIFICANCE: These patient-preference disability

  9. Induction of mutation with gamma irradiation in camellia (Camellia Japonica L.)

    International Nuclear Information System (INIS)

    Zhou Diying; Shen Shoujiang; Wang Yanjun

    1990-01-01

    Studies on the radiosensitivity and mutagenic effects in Camellia (Camellia Japonica L.) were carried out with the shoot cuttings, one-year seedlings and three or four-year plants irradiated with 60 Co-γ rays. Results obtained showed that the radiosensitivities varied greatly in cultivars and in different parts of the plant. The suitable irradiation doses for shoot cuttings are 1-3 krad. The treatment of low doses (bellow 2 kard) resulted in higher frequency of mutation with plants of high sensitivity to γ-rays. The frequencies of mutation in flower color and type were 2.5-12.0%. The mutant with ornamental value has been obtained

  10. DNA variation in the phenotypically-diverse brown alga Saccharina japonica

    Directory of Open Access Journals (Sweden)

    Balakirev Evgeniy S

    2012-07-01

    Full Text Available Abstract Background Saccharina japonica (Areschoug Lane, Mayes, Druehl et Saunders is an economically important and highly morphologically variable brown alga inhabiting the northwest Pacific marine waters. On the basis of nuclear (ITS, plastid (rbcLS and mitochondrial (COI DNA sequence data, we have analyzed the genetic composition of typical Saccharina japonica (TYP and its two common morphological varieties, known as the “longipes” (LON and “shallow-water” (SHA forms seeking to clarify their taxonomical status and to evaluate the possibility of cryptic species within S. japonica. Results The data show that the TYP and LON forms are very similar genetically in spite of drastic differences in morphology, life history traits, and ecological preferences. Both, however, are genetically quite different from the SHA form. The two Saccharina lineages are distinguished by 109 fixed single nucleotide differences as well as by seven fixed length polymorphisms (based on a 4,286 bp concatenated dataset that includes three gene regions. The GenBank database reveals a close affinity of the TYP and LON forms to S. japonica and the SHA form to S. cichorioides. The three gene markers used in the present work have different sensitivity for the algal species identification. COI gene was the most discriminant gene marker. However, we have detected instances of interspecific COI recombination reflecting putative historical hybridization events between distantly related algal lineages. The recombinant sequences show highly contrasted level of divergence in the 5’- and 3’- regions of the gene, leading to significantly different tree topologies depending on the gene segment (5’- or 3’- used for tree reconstruction. Consequently, the 5’-COI “barcoding” region (~ 650 bp can be misleading for identification purposes, at least in the case of algal species that might have experienced historical hybridization events. Conclusion Taking into account

  11. Use of gamma-rays sensitivity for discrimination of upland cultivars of groups Indican and Japonica

    International Nuclear Information System (INIS)

    Rodrigues, Luis Roberto Franco

    2003-01-01

    Sixty-five upland rice cultivars (Oryza sativa L.) were evaluated in relation to gamma-ray sensitivity. Seeds were subjected to seven doses of gamma-radiation and sown in wooden boxes in randomised complete block design with three replications. The experiment was conducted in greenhouse during the year of 1992. Physiological effects caused by radiation in the M 1 generation, were evaluated. The results showed that the sensitivity to the radiation at doses 300 and 360 Gy was useful for distinguishing Indican and Japonica groups. (author)

  12. Two neutral alleles of improving male gamete abortion in indica-japonica hybrid rice

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@It was difficult to use the pronounced heterosis of indica-japonica hybrids rice due to the panicle sterility caused by male and female gamete abortion. The female gamete abortion in most of subspecific hybrids could be solved by using an abortion-neutral gene S5-n, a wide compatibility gene. The problem of male gamete abortion indicated by distorted segregation of marker genes remained to be studied. Segregation distortion via male gamete had been reported on chromosomes 3, 7, 8, 11, and 12.

  13. DNA damage in gill cells of Corbicula japonica exposed to natural and anthropogenic stressors

    Directory of Open Access Journals (Sweden)

    Valentina Vladimirovna Slobodskova

    2015-06-01

    The results are presented as the percentage distribution of nuclei in the various damage classes and summarized in an index of DNA integrity or genetic damage index GDI (Cavas, Kohen, 2008: The results from our study showed significant level of DNA damage from the C. japonica which were collected from polluted sites. Unpolluted sites were described as with no or minimal DNA strand breaks. Mollusks collected at polluted areas (estuary Razdolnaya river, lagoon Tihaya showed high levels of DNA damage, GDI is equal to 3.22±0.2 and 3.11±0.7 in gills respectively. C. japonica obtained from a ‘clean’ areas (estuary Artemovka river, estuary Partizanskaya river demonstrate less high level of DNA damage destruction, GDI is equal to 0,6±0,08 and 0,71±0,12 in gills respectively. Lipid peroxidation level was assayed measurement of malondialdehyde (MDA, a decomposition product of polyunsaturated fatty acids hydro peroxides were determined by the TBA reaction. The absorbance was read at 532 nm after removal of substances (TBARS formed was calculated by using an extinction coefficient of 1.56*105 M-1 cm -1 formed per g dry weight. C. japonica sampled at Artemovka estuary, Partizanskaya estuary, Razdolnaya estuary, Tihaya lagoon showed LPX level (3.46±0.59, 5.62±0.82, 12.85±0.52, 15.32±1.13 nmol TBARS/g dry wt in gills respectively. In conclusion, it can be noted that in the course of the experiment we found a clear relationship between the amount of DNA damage and the level of peroxidation products (MDA in the gills of bivalve C. japonica, collected from sites with varying degrees of anthropogenic load. It should be emphasized that the shellfish that live in polluted areas are likely to be in a state of oxidative stress, which is one of the causes the degradation of DNA.

  14. Microtropiosides A-F: ent-Labdane diterpenoid glucosides from the leaves of Microtropis japonica (Celastraceae).

    Science.gov (United States)

    Koyama, Yuka; Matsunami, Katsuyoshi; Otsuka, Hideaki; Shinzato, Takakazu; Takeda, Yoshio

    2010-04-01

    From a 1-BuOH-soluble fraction of a MeOH extract of the leaves of Microtropis japonica, collected in the Okinawa islands, six ent-labdane glucosides, named microtropiosides A-F, were isolated together with one known acyclic sesquiterpene glucoside. Their structures were elucidated by a combination of spectroscopic analyses, and their absolute configurations determined by application of the beta-D-glucopyranosylation-induced shift-trend rule in (13)C NMR spectroscopy and the modified Mosher's method. Copyright 2010 Elsevier Ltd. All rights reserved.

  15. The breeding of Japonica Yanjing 10 rice mutant induced by space mutation

    International Nuclear Information System (INIS)

    Wang Jianhua; Chen Xiulan; Zhang Rong; Wang Jinrong; Liu Jian; Jiao Juan; He Zhentian; Wang Lin

    2011-01-01

    The dry seed of mid-maturing Japonica rice Yanjing 10 was used for space mutation breeding which was carried by a satellite for 15 days in 2006. Through three generations of breeding, a group of mutants were obtained. In the article, we reported in detail the breeding procedures, proposed the breeding technical method for space mutation for rice improvement. Planting multiple seedlings per hill to prohibit tillering at SP 1 generation, and bulked selection in combination with directional selection at the SP 2 ∼ SP 3 generation were the two key points of the breeding methods. (authors)

  16. Social Change of Bajo Tribe Society in Karimunjawa: From "Sea Tribe" to "Land Tribe"

    Directory of Open Access Journals (Sweden)

    Titiek Suliyati

    2017-12-01

    The result of the research shows that there is a social change in Bajo society living permanently in Karimunjawa that is, the change of daily behavior in the society, social interaction with other tribes, values held by the society and social institution, structure and social classes. Social change occurring to Bajo society in Karimunjawa brings positive influences. The social changes among others are awareness towards the importance of education, Bajo society has new jobs other than fisherman, the increase of income, living standard, also modernization in fisheries system. The negative impact as a consequence of the social changes is faded culture, changes in life orientation and views of life, and consumerism in the society.

  17. Condiciones de trabajo materno y bajo peso al nacer en la Ciudad de México

    Directory of Open Access Journals (Sweden)

    CERÓN-MIRELES PRUDENCIA

    1997-01-01

    Full Text Available Objetivo. Estudiar la asociación entre condiciones de trabajo materno y bajo peso al nacer en la Ciudad de México. Material y métodos. Se encuestaron 2 623 trabajadoras que atendieron sus partos en tres grandes hospitales de la Ciudad de México en 1992. Se obtuvo información sobre los principales factores biológicos y sociales que se asocian al bajo peso al nacer; asimismo, se determinó el estrés ocupacional utilizando el instrumento desarrollado por Karasek. Se utilizaron modelos de regresión logística para evaluar la relación de las condiciones de trabajo con el bajo peso al nacer, controlando por variables confusoras. Resultados. El bajo peso al nacer fue más alto en trabajadoras con jornadas mayores a 50 horas semanales (RM= 1.6; IC= 1.17, 2.28 y con conflictos laborales (RM= 1.5; IC= 1.0, 2.25. La falta de apoyo social tangible fue identificado como un factor de riesgo de bajo peso al nacer (RM= 1.7; IC= 1.20, 2.33. Las medidas preventivas laborales como el cambio de tareas, disminución de la jornada y las salidas por enfermedad no mostraron un efecto benéfico en el peso al nacer, a excepción de la licencia de maternidad; las madres que no la tuvieron mostraron una probabilidad 2.2 veces mayor para bajo peso al nacer (IC= 1.66, 2.93. Conclusiones. Estos hallazgos resaltan la importancia de identificar los factores de riesgo ocupacional en el embarazo..

  18. Serie Madera para construcción La madera certificada bajo el concepto del triple resultado

    OpenAIRE

    Camacho-Cornejo, Diego; Moya-Roque, Róger

    2012-01-01

    A nivel mundial existe una tendencia de utilización de los productos certificados de madera bajo el concepto de triple resultado. En Costa Rica, diferentes empresas trabajan bajo este concepto. Es por ello que el presente trabajo tiene como objetivo mostrar el desarrollo de la madera certificada en Costa Rica, estableciendo la importancia de utilizar este tipo de madera, las ventajas de utilizarla en relación a aquella que no presenta esta certificación. Así mismo se presenta un detalle de la...

  19. LA INSATISFACCIÓN LABORAL COMO FACTOR DEL BAJO RENDIMIENTO DEL TRABAJADOR

    OpenAIRE

    Granda Carazas, Segundo Eloy

    2014-01-01

    El tema "la insatisfacción laboral como factor del bajo rendimiento del trabajador" es importante, ya que se puede constatar que en algunas organizaciones, pese a sus significativos esfuerzos para ampliar y modernizar su infraestructura, afrontan problemas de rendimiento laboral. Este artículo se orienta a determinar en qué medida la insatisfacción laboral se asocia al bajo rendimiento laboral en una organización. Como factores constituyentes de la insatisfacción laboral se considerar...

  20. Effects of Laminaria japonica polysaccharides on exercise endurance and oxidative stress in forced swimming mouse model.

    Science.gov (United States)

    Yan, Feiwei; Hao, Haitao

    2016-12-01

    Polysaccharides are the major active ingredients responsible for the bioactivities of Laminaria japonica. However, the effects of L. japonica polysaccharides (LJP) on exercise endurance and oxidative stress have never been investigated. Therefore, this study was conducted to investigate the effects of LJP on exercise endurance and oxidative stress in a forced swimming mouse model. The animals were divided into four groups, namely the control (C), LJP-75, LJP-150, and LJP-300 groups, which received physiological saline and 75, 150, and 300 mg kg(-1) LJP, respectively, by gavage once a day for 28 days. This was followed by a forced swimming test and measurements of various biochemical parameters. LJP increased swimming time to exhaustion, the liver and muscle glycogen content, and levels of superoxide dismutase, glutathione peroxidase, and catalase in the serum, liver, and muscle, which were accompanied by corresponding decreases in the malondialdehyde (MDA) content of the same tissues. Furthermore, decreases in blood lactic acid and serum myeloperoxidase (MPO) levels were observed. LJP enhanced exercise endurance and protected mice against exhaustive exercise-induced oxidative stress.

  1. Rabdosia japonica var. glaucocalyx Flavonoids Fraction Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Mice

    Directory of Open Access Journals (Sweden)

    Chun-jun Chu

    2014-01-01

    Full Text Available Rabdosia japonica var. glaucocalyx (Maxim. Hara, belonging to the Labiatae family, is widely used as an anti-inflammatory and antitumor drug for the treatment of different inflammations and cancers. Aim of the Study. To investigate therapeutic effects and possible mechanism of the flavonoids fraction of Rabdosia japonica var. glaucocalyx (Maxim. Hara (RJFs in acute lung injury (ALI mice induced by lipopolysaccharide (LPS. Materials and Methods. Mice were orally administrated with RJFs (6.4, 12.8, and 25.6 mg/kg per day for 7 days, consecutively, before LPS challenge. Lung specimens and the bronchoalveolar lavage fluid (BALF were isolated for histopathological examinations and biochemical analysis. The level of complement 3 (C3 in serum was quantified by a sandwich ELISA kit. Results. RJFs significantly attenuated LPS-induced ALI via reducing productions of the level of inflammatory mediators (TNF-α, IL-6, and IL-1β, and significantly reduced complement deposition with decreasing the level of C3 in serum, which was exhibited together with the lowered myeloperoxidase (MPO activity and nitric oxide (NO and protein concentration in BALF. Conclusions. RJFs significantly attenuate LPS-induced ALI via reducing productions of proinflammatory mediators, decreasing the level of complement, and reducing radicals.

  2. Identification of Insecticidal Constituents from the Essential Oil from the Aerial Parts Stachys riederi var. japonica.

    Science.gov (United States)

    Quan, Meirong; Liu, Qi Zhi; Liu, Zhi Long

    2018-05-17

    The essential oil of Stachys riederi var. japonica (Family: Lamiaceae) was extracted by hydrodistillation and determined by GC and GC-MS. A total of 40 components were identified, representing 96.01% of the total oil composition. The major compounds in the essential oil were acetanisole (15.43%), anisole (9.43%), 1,8-cineole (8.07%), geraniol (7.89%), eugenol (4.54%), caryophyllene oxide (4.47%), caryophyllene (4.21%) and linalool (4.07%). Five active constituents (acetanisole, anisole, 1,8-cineole, eugenol and geraniol) were identified by bioactivity-directed fractionation. The essential oil possessed fumigant toxicity against maize weevils ( Sitophilus zeamais ) and booklice ( Liposcelis bostrychophila ), with LC 50 values of 15.0 mg/L and 0.7 mg/L, respectively. Eugenol and anisole exhibited stronger fumigant toxicity than the oil against booklice. 1,8-Cineole showed stronger toxicity, and anisole as well as eugenol exhibited the same level of fumigant toxicity as the essential oil against maize weevils. The essential oil also exhibited contact toxicity against S. zeamais adults and L. bostrychophila , with LC 50 values of 21.8 µg/adult and 287.0 µg/cm², respectively. The results indicated that the essential oil of S. riederi var. japonica and its isolates show potential as fumigants, and for their contact toxicity against grain storage insects.

  3. Antiatherogenic Effect of Camellia japonica Fruit Extract in High Fat Diet-Fed Rats

    Directory of Open Access Journals (Sweden)

    Hyun-Ho Lee

    2016-01-01

    Full Text Available Hypercholesterolemia is a well-known etiological factor for cardiovascular disease and a common symptom of most types of metabolic disorders. Camellia japonica is a traditional garden plant, and its flower and seed have been used as a base oil of traditional cosmetics in East Asia. The present study was carried out to evaluate the effect of C. japonica fruit extracts (CJF in a high fat diet- (HFD- induced hypercholesterolemic rat model. CJF was administered orally at three different doses: 100, 400, and 800 mg·kg−1·day−1 (CJF 100, 400, and 800, resp.. Our results showed that CJF possessed strong cholesterol-lowering potency as indicated by the decrease in serum total cholesterol (TC, triglyceride (TG, and low-density lipoprotein (LDL, accompanied by an increase in serum high-density lipoprotein (HDL. Furthermore, CJF reduced serum lipid peroxidation by suppressing the formation of thiobarbituric acid reactive substance. In addition, oil red O (ORO staining of rat arteries showed decreased lipid-positive staining in the CJF-treated groups compared to the control HFD group. Taken together, these results suggest that CJF could be a potent herbal therapeutic option and source of a functional food for the prevention and treatment of atherosclerosis and other diseases associated with hypercholesterolemia.

  4. Accumulation and tolerance characteristics of cadmium in a potential hyperaccumulator-Lonicera japonica Thunb

    Energy Technology Data Exchange (ETDEWEB)

    Liu Zhouli [Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, Shenhe District, Shenyang 110016 (China); Graduate School, Chinese Academy of Sciences, Jia 19 Yuquan Road, Beijing 100039 (China); He Xingyuan, E-mail: hexy@iae.ac.cn [Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, Shenhe District, Shenyang 110016 (China); Chen Wei; Yuan Fenghui [Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, Shenhe District, Shenyang 110016 (China); Yan Kun [Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, Shenhe District, Shenyang 110016 (China); Graduate School, Chinese Academy of Sciences, Jia 19 Yuquan Road, Beijing 100039 (China); Tao Dali [Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, Shenhe District, Shenyang 110016 (China)

    2009-09-30

    Phytoremediation using hyperaccumulators is a promising technique of removing soil pollutants. In the study, growth responses, cadmium (Cd) accumulation capability and physiological mechanisms of Lonicera japonica Thunb. under Cd stress were investigated. Exposed to 5 and 10 mg L{sup -1} Cd, the plants did not show any visual symptoms, furthermore, the height, dry biomass of leaves, roots and total and the chlorophyll (CHL) content were obtained different grade increase. When the concentration of Cd was up to 50 mg L{sup -1}, the height, dry biomass of leaves and roots had not significant differences compared with the control. The indexes of tolerance (IT) were all above 0.8. The maintenance of high superoxide dismutase (SOD) and catalase (CAT) activities was observed along with the increased Cd concentration, suggesting strong internal detoxification mechanisms inside plant cells. After 21 days exposure to 25 mg L{sup -1} Cd, stem and shoot Cd concentrations reached 344.49 {+-} 0.71 and 286.12 {+-} 9.38 {mu}g g{sup -1} DW, respectively and the plant had higher bioaccumulation coefficient (BC) and translocation factor (TF). According to these results, it was shown L. japonica had strong tolerance and accumulation capability to Cd, therefore it is a potential Cd-hyperaccumulator.

  5. Nigerian Journal of Animal Production - Vol 42, No 2 (2015)

    African Journals Online (AJOL)

    Performance and carcass values of Japanese quails (Coturnix coturnix japonica) fed processed sweet potato (Ipomea batatas) meal diets · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. JA Edache, CD Tuleun, RU Muduudtai, AG Yisa, 45-57 ...

  6. Maternal influence on eggshell maculation: implications for cryptic camouflaged eggs

    Czech Academy of Sciences Publication Activity Database

    Duval, C.; Cassey, P.; Lovell, P.G.; Mikšík, Ivan; Reynolds, S.J.; Spencer, K.A.

    2016-01-01

    Roč. 157, č. 1 (2016), s. 303-310 ISSN 0021-8375 R&D Projects: GA ČR(CZ) GA15-01948S Institutional support: RVO:67985823 Keywords : body condition * camouflage * Coturnix coturnix japonica * eggs hell maculation * Protoporphyrin Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 1.468, year: 2016

  7. The effect of long-term feeding of conjugated linoleic acid on fertility ...

    African Journals Online (AJOL)

    The objective of this study was to determine the effects of the long-term feeding of conjugated linoleic acid (CLA) on laying hen performance, egg fertility and hatchability of fertile eggs of Japanese quail (Coturnix coturnix japonica). One hundred and sixty 7-day old Japanese quail chicks were randomly assigned to four ...

  8. Effect of dietary supplementation of olive leaves on performance and ...

    African Journals Online (AJOL)

    Λευτέρης

    cholesterol and triglycerides concentrations were determined. ... Keywords: Pimpinella anisum, vitamin E, Coturnix japonica, performance, egg .... 8 mg vitamin B2; 3 mg vitamin B6; 20 μg vitamin B12; 85 mg vitamin niacin; 20 mg pantothenic.

  9. Protective effects against H2O2-induced damage by enzymatic hydrolysates of an edible brown seaweed, sea tangle (Laminaria japonica).

    Science.gov (United States)

    Park, Pyo-Jam; Kim, Eun-Kyung; Lee, Seung-Jae; Park, Sun-Young; Kang, Dong-Soo; Jung, Bok-Mi; Kim, Kui-Shik; Je, Jae-Young; Ahn, Chang-Bum

    2009-02-01

    Enzymatic hydrolysates of Laminaria japonica were evaluated for antioxidative activities using hydroxyl radical scavenging activity and protective effects against H(2)O(2)-induced DNA and cell damage. In addition, activities of antioxidative enzymes, including catalase, glutathione peroxidase, and glutathione S-transferase, of the enzymatic hydrolysates from L. japonica were also estimated. L. japonica was first enzymatically hydrolyzed by seven carbohydrases (Dextrozyme, AMG, Promozyme, Maltogenase, Termamyl, Viscozyme, and Celluclast [all from Novo Co., Novozyme Nordisk, Bagsvaerd, Denmark]) and five proteinases (Flavourzyme, Neutrase, Protamex, Alcalase [all from Novo Co.], and pancreatic trypsin). The hydroxyl radical scavenging activities of Promozyme and pancreatic trypsin hydrolysates from L. japonica were the highest as compared to those of the other carbohydrases and proteinases, and their 50% inhibitory concentration values were 1.67 and 317.49 mug/mL, respectively. The pancreatic trypsin hydrolysates of L. japonica exerted a protective effect on H(2)O(2)-induced DNA damage. We also evaluated the protective effect on hydroxyl radical-induced oxidative damage in PC12 cells via propidium iodide staining using a flow cytometer. The AMG and pancreatic trypsin hydrolysates of L. japonica dose-dependently protected PC12 cells against cell death caused by hydroxyl radical-induced oxidative damage. Additionally, we analyzed the activity of antioxidative enzymes such as catalase, glutathione peroxidase, and the phase II biotransformation enzyme glutathione S-transferase in L. japonica-treated cells. The activity of all antioxidative enzymes was higher in L. japonica-treated cells compared with the nontreated cells. These results indicate that enzymatic hydrolysates of L. japonica possess antioxidative activity.

  10. Optimizing soaking and germination conditions to improve gamma-aminobutyric acid content in japonica and indica germinated brown rice

    NARCIS (Netherlands)

    Zhang, Q.; Xiang, J.; Zhang, L.; Zhu, X.; Evers, J.B.; Werf, van der W.; Duan, L.

    2014-01-01

    Germinated brown rice is a well-known functional food due to its high content of gamma-aminobutyric acid (GABA). This study was designed to test the difference of producing GABA in two domesticated rice genotypes (indica and japonica rice), and the effects of adding exogenous glutamic acid or

  11. Sympatric spawning but allopatric distribution of Anguilla japonica and Anguilla marmorata: temperature- and oceanic current-dependent sieving.

    Directory of Open Access Journals (Sweden)

    Yu-San Han

    Full Text Available Anguilla japonica and Anguilla marmorata share overlapping spawning sites, similar drifting routes, and comparable larval durations. However, they exhibit allopatric geographical distributions in East Asia. To clarify this ecological discrepancy, glass eels from estuaries in Taiwan, the Philippines, Indonesia, and China were collected monthly, and the survival rate of A. marmorata under varying water salinities and temperatures was examined. The composition ratio of these 2 eel species showed a significant latitude cline, matching the 24 °C sea surface temperature isotherm in winter. Both species had opposing temperature preferences for recruitment. A. marmorata prefer high water temperatures and die at low water temperatures. In contrast, A. japonica can endure low water temperatures, but their recruitment is inhibited by high water temperatures. Thus, A. japonica glass eels, which mainly spawn in summer, are preferably recruited to Taiwan, China, Korea, and Japan by the Kuroshio and its branch waters in winter. Meanwhile, A. marmorata glass eels, which spawn throughout the year, are mostly screened out in East Asia in areas with low-temperature coastal waters in winter. During summer, the strong northward currents from the South China Sea and Changjiang River discharge markedly block the Kuroshio invasion and thus restrict the approach of A. marmorata glass eels to the coasts of China and Korea. The differences in the preferences of the recruitment temperature for glass eels combined with the availability of oceanic currents shape the real geographic distribution of Anguilla japonica and Anguilla marmorata, making them "temperate" and "tropical" eels, respectively.

  12. Predicting the spatial distribution of Lonicera japonica, based on species occurrence data from two watersheds in Western Kentucky and Tennessee

    Science.gov (United States)

    Dongjiao Liu; Hao Jiang; Robin Zhang; Kate S. He

    2011-01-01

    The spatial distribution of most invasive plants is poorly documented and studied. This project examined and compared the spatial distribution of a successful invasive plant, Japanese honeysuckle (Lonicera japonica), in two similar-sized but ecologically distinct watersheds in western Kentucky (Ledbetter Creek) and western Tennessee (Panther Creek)....

  13. Sympatric spawning but allopatric distribution of Anguilla japonica and Anguilla marmorata: temperature- and oceanic current-dependent sieving.

    Science.gov (United States)

    Han, Yu-San; Yambot, Apolinario V; Zhang, Heng; Hung, Chia-Ling

    2012-01-01

    Anguilla japonica and Anguilla marmorata share overlapping spawning sites, similar drifting routes, and comparable larval durations. However, they exhibit allopatric geographical distributions in East Asia. To clarify this ecological discrepancy, glass eels from estuaries in Taiwan, the Philippines, Indonesia, and China were collected monthly, and the survival rate of A. marmorata under varying water salinities and temperatures was examined. The composition ratio of these 2 eel species showed a significant latitude cline, matching the 24 °C sea surface temperature isotherm in winter. Both species had opposing temperature preferences for recruitment. A. marmorata prefer high water temperatures and die at low water temperatures. In contrast, A. japonica can endure low water temperatures, but their recruitment is inhibited by high water temperatures. Thus, A. japonica glass eels, which mainly spawn in summer, are preferably recruited to Taiwan, China, Korea, and Japan by the Kuroshio and its branch waters in winter. Meanwhile, A. marmorata glass eels, which spawn throughout the year, are mostly screened out in East Asia in areas with low-temperature coastal waters in winter. During summer, the strong northward currents from the South China Sea and Changjiang River discharge markedly block the Kuroshio invasion and thus restrict the approach of A. marmorata glass eels to the coasts of China and Korea. The differences in the preferences of the recruitment temperature for glass eels combined with the availability of oceanic currents shape the real geographic distribution of Anguilla japonica and Anguilla marmorata, making them "temperate" and "tropical" eels, respectively.

  14. PRODUCTION ECOLOGY OF THE NON-INDIGENOUS SEAGRASS, DWARF EELGRASS (ZOSTERA JAPONICA ASCHER. & GRAEB.), IN A PACIFIC NORTHWEST ESTUARY, USA

    Science.gov (United States)

    The non-indigenous seagrass Zostera japonica Ascher. & Graeb. (dwarf eelgrass) was first identified in central Oregon (USA) estuaries about 30 years ago. The autecology of this species is poorly described at the southern end of its non-native range although several process orien...

  15. Bacterial community composition in the gut content of Lampetra japonica revealed by 16S rRNA gene pyrosequencing.

    Science.gov (United States)

    Zuo, Yu; Xie, Wenfang; Pang, Yue; Li, Tiesong; Li, Qingwei; Li, Yingying

    2017-01-01

    The composition of the bacterial communities in the hindgut contents of Lampetrs japonica was surveyed by Illumina MiSeq of the 16S rRNA gene. An average of 32385 optimized reads was obtained from three samples. The rarefaction curve based on the operational taxonomic units tended to approach the asymptote. The rank abundance curve representing the species richness and evenness was calculated. The composition of microbe in six classification levels was also analyzed. Top 20 members in genera level were displayed as the classification tree. The abundance of microorganisms in different individuals was displayed as the pie charts at the branch nodes in the classification tree. The differences of top 50 genera in abundance between individuals of lamprey are displayed as a heatmap. The pairwise comparison of bacterial taxa abundance revealed that there are no significant differences of gut microbiota between three individuals of lamprey at a given rarefied depth. Also, the gut microbiota derived from L. japonica displays little similarity with other aquatic organism of Vertebrata after UPGMA analysis. The metabolic function of the bacterial communities was predicted through KEGG analysis. This study represents the first analysis of the bacterial community composition in the gut content of L. japonica. The investigation of the gut microbiota associated with L. japonica will broaden our understanding of this unique organism.

  16. Bacterial community composition in the gut content of Lampetra japonica revealed by 16S rRNA gene pyrosequencing.

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    Yu Zuo

    Full Text Available The composition of the bacterial communities in the hindgut contents of Lampetrs japonica was surveyed by Illumina MiSeq of the 16S rRNA gene. An average of 32385 optimized reads was obtained from three samples. The rarefaction curve based on the operational taxonomic units tended to approach the asymptote. The rank abundance curve representing the species richness and evenness was calculated. The composition of microbe in six classification levels was also analyzed. Top 20 members in genera level were displayed as the classification tree. The abundance of microorganisms in different individuals was displayed as the pie charts at the branch nodes in the classification tree. The differences of top 50 genera in abundance between individuals of lamprey are displayed as a heatmap. The pairwise comparison of bacterial taxa abundance revealed that there are no significant differences of gut microbiota between three individuals of lamprey at a given rarefied depth. Also, the gut microbiota derived from L. japonica displays little similarity with other aquatic organism of Vertebrata after UPGMA analysis. The metabolic function of the bacterial communities was predicted through KEGG analysis. This study represents the first analysis of the bacterial community composition in the gut content of L. japonica. The investigation of the gut microbiota associated with L. japonica will broaden our understanding of this unique organism.

  17. Essential Oil from Cryptomeria japonica Induces Apoptosis in Human Oral Epidermoid Carcinoma Cells via Mitochondrial Stress and Activation of Caspases

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    Ji-Young Kim

    2012-03-01

    Full Text Available Cryptomeria japonica D. Don (C. japonica has been used in traditional medicines from Asia for a variety of indications, including liver ailments, and an antitussive, and for its antiulcer activities. We examined the cell viability and apoptosis of KB cells treated with C. japonica essential oil at several concentrations for 12 h by MTT assay, Hoechst-33258 dye staining, DNA fragmentation, flow cytometry (cell cycle, and Western blotting for mitochondria stress, activation of caspases, and poly (ADP-ribose polymerase. The essential oil induced the apoptosis of KB cells in a dose-dependent manner, which was verified by DNA fragmentation, appearance of apoptotic bodies, and the sub-G1 ratio. The essential oil also induced rapid and transient caspase-3 activity and cleavage of PARP of the KB cells. Treating the cells with the oil also caused changes in the mitochondrial level of the Bcl-2 family proteins such as Bcl-2 and Bax, thereby inducing the release of cytochrome c into the cytosol. The essential oil of C. japonica may have potential as a cancer chemopreventive and therapeutic agent.

  18. Naturally occurrence of Sr and Ca in the stem of a Japanese cedar (Cryptomeria japonica D. Don) using PIXE

    International Nuclear Information System (INIS)

    Katayama, Y.; Aoki, T.; Ko, S.; Yoshida, K.

    2000-01-01

    Distribution profiles of Sr and Ca occurring naturally in the stem of a Japanese cedar (Cryptomeria japonica D. Don) were determined using PIXE. The average concentration of Sr was around 7.5 ppm and the average Ca concentration was about 540 ppm. (author)

  19. Identity and specificity of Rhizoctonia-like fungi from different populations of Liparis japonica (Orchidaceae) in Northeast China.

    Science.gov (United States)

    Ding, Rui; Chen, Xu-Hui; Zhang, Li-Jun; Yu, Xiao-Dan; Qu, Bo; Duan, Ru; Xu, Yu-Feng

    2014-01-01

    Mycorrhizal association is known to be important to orchid species, and a complete understanding of the fungi that form mycorrhizas is required for orchid ecology and conservation. Liparis japonica (Orchidaceae) is a widespread terrestrial photosynthetic orchid in Northeast China. Previously, we found the genetic diversity of this species has been reduced recent years due to habitat destruction and fragmentation, but little was known about the relationship between this orchid species and the mycorrhizal fungi. The Rhizoctonia-like fungi are the commonly accepted mycorrhizal fungi associated with orchids. In this study, the distribution, diversity and specificity of culturable Rhizoctonia-like fungi associated with L. japonica species were investigated from seven populations in Northeast China. Among the 201 endophytic fungal isolates obtained, 86 Rhizoctonia-like fungi were identified based on morphological characters and molecular methods, and the ITS sequences and phylogenetic analysis revealed that all these Rhizoctonia-like fungi fell in the same main clade and were closely related to those of Tulasnella calospora species group. These findings indicated the high mycorrhizal specificity existed in L. japonica species regardless of habitats at least in Northeast China. Our results also supported the wide distribution of this fungal partner, and implied that the decline of L. japonica in Northeast China did not result from high mycorrhizal specificity. Using culture-dependent technology, these mycorrhizal fungal isolates might be important sources for the further utilizing in orchids conservation.

  20. Metal accumulation in the polychaete Hediste japonica with emphasis on interaction between heavy metals and petroleum hydrocarbons

    International Nuclear Information System (INIS)

    Sun Fuhong; Zhou Qixing

    2007-01-01

    The accumulation of cadmium (Cd) and copper (Cu) in the polychaete Hediste japonica exposed to the mixture of Cd (or Cu) and petroleum hydrocarbons (PHCs) was investigated and compared with that exposed to single Cd (or Cu). The increased bioavailability of Cd or Cu with exposure concentrations resulted in an increase in the accumulation and net accumulation rate of Cd or Cu during single metal exposure. The net accumulation rate of Cd increased, but the net accumulation rate of Cu decreased with exposure time during single metal exposure, suggesting that H. japonica could actively regulate Cu burden in their body by inhibition of absolute uptake or promotion of excretion. The interactions between Cd (or Cu) and PHCs had complicated influences on the net accumulation rate of Cd and Cu in H. japonica under the condition of the binary mixture, which are dependent on their concentration combinations and exposure time. - The influences of petroleum hydrocarbons on Cd and Cu accumulation in H. japonica depend on their concentration combinations and exposure time

  1. Identity and specificity of Rhizoctonia-like fungi from different populations of Liparis japonica (Orchidaceae in Northeast China.

    Directory of Open Access Journals (Sweden)

    Rui Ding

    Full Text Available Mycorrhizal association is known to be important to orchid species, and a complete understanding of the fungi that form mycorrhizas is required for orchid ecology and conservation. Liparis japonica (Orchidaceae is a widespread terrestrial photosynthetic orchid in Northeast China. Previously, we found the genetic diversity of this species has been reduced recent years due to habitat destruction and fragmentation, but little was known about the relationship between this orchid species and the mycorrhizal fungi. The Rhizoctonia-like fungi are the commonly accepted mycorrhizal fungi associated with orchids. In this study, the distribution, diversity and specificity of culturable Rhizoctonia-like fungi associated with L. japonica species were investigated from seven populations in Northeast China. Among the 201 endophytic fungal isolates obtained, 86 Rhizoctonia-like fungi were identified based on morphological characters and molecular methods, and the ITS sequences and phylogenetic analysis revealed that all these Rhizoctonia-like fungi fell in the same main clade and were closely related to those of Tulasnella calospora species group. These findings indicated the high mycorrhizal specificity existed in L. japonica species regardless of habitats at least in Northeast China. Our results also supported the wide distribution of this fungal partner, and implied that the decline of L. japonica in Northeast China did not result from high mycorrhizal specificity. Using culture-dependent technology, these mycorrhizal fungal isolates might be important sources for the further utilizing in orchids conservation.

  2. Diaphorina citri Induces Huanglongbing-Infected Citrus Plant Volatiles to Repel and Reduce the Performance of Propylaea japonica.

    Science.gov (United States)

    Lin, Yongwen; Lin, Sheng; Akutse, Komivi S; Hussain, Mubasher; Wang, Liande

    2016-01-01

    Transmission of plant pathogens through insect vectors is a complex biological process involving interactions between the host plants, insects, and pathogens. Simultaneous impact of the insect damage and pathogenic bacteria in infected host plants induce volatiles that modify not only the behavior of its insect vector but also of their natural enemies, such as parasitoid wasps. Therefore, it is essential to understand how insects such as the predator ladybird beetle responds to volatiles emitted from a host plant and how the disease transmission alters the interactions between predators, vector, pathogens, and plants. In this study, we investigated the response of Propylaea japonica to volatiles from citrus plants damaged by Diaphorina citri and Candidatus Liberibacter asiaticus through olfactometer bioassays. Synthetic chemical blends were also used to determine the active compounds in the plant volatile. The results showed that volatiles emitted by healthy plants attracted more P. japonica than other treatments, due to the presence of high quantities of D-limonene and beta-ocimene, and the lack of methyl salicylate. When using synthetic chemicals in the olfactory tests, we found that D-limonene attracted P. japonica while methyl salicylate repelled the predator. However, beta-ocimene attracted the insects at lower concentrations but repelled them at higher concentrations. These results indicate that P. japonica could not efficiently search for its host by using volatile cues emitted from psyllids- and Las bacteria-infected citrus plants.

  3. Estratificación del bajo peso al nacer desde un enfoque de determinantes sociales

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    Hilda María Delgado Acosta

    2013-03-01

    Full Text Available Fundamento: el área materno-infantil requiere de un enfoque en sus determinantes para aumentar el alcance estratégico de sus programas. Objetivo: estratificar el bajo peso al nacer en el municipio de Cienfuegos y caracterizar el bajo peso de acuerdo a determinantes sociales seleccionadas. Métodos: se realizó un estudio epidemiológico, descriptivo, correlacional sobre la estratificación del bajo peso al nacer en los consejos populares del municipio Cienfuegos, en el período comprendido entre los años 2003 y 2007. Los estratos se definieron según el valor de la media del índice de bajo peso al nacer en este periodo. Para realizar el análisis de las variables cualitativas en muestras independientes se utilizó la prueba de independencia u homogeneidad utilizada en el caso de dos muestras con resultado dicotómico o más. El procesamiento estadístico se realizó en el programa SPSS versión 15,0 y se utilizaron como medidas matemáticas para las variables cualitativas el porcentaje, y para las variables cuantitativas la media aritmética. La técnica estadística para relacionar las variables fue la prueba Chi-cuadrado. Resultados: se mostraron diferencias estadísticamente significativas entre los estratos de bajo, mediano y alto riesgo, en el nivel de escolaridad, estado civil, ocupación, evaluación nutricional, hábito de fumar, antecedentes de bajo peso al nacer, período ínter genésico. La única enfermedad asociada que mostró diferencias entre ellos fue la hipertensión gestacional. Conclusiones: el bajo peso al nacer continúa siendo un serio problema de salud en el municipio Cienfuegos, cuestión esta que debe ser abordada desde sus determinantes para realizar intervenciones futuras y el logro de mejores resultados.

  4. Factores nutricionales maternos y el bajo peso al nacer en un área de salud

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    Arley Rey Vilchez

    2013-01-01

    Full Text Available Fundamento: el bajo peso al nacer es multifactorial y se atribuye a causas maternas, fetales y ambientales. Los hábitos alimentarios inadecuados unidos a otros factores nutricionales maternos son determinantes. Objetivo: identificar los factores nutricionales maternos relacionados con el bajo peso al nacer en pacientes del Área I del municipio Cienfuegos. Métodos: se realizó un estudio analítico, retrospectivo, de casos y controles entre enero 2006 y diciembre 2008. Se estudiaron 50 madres de recién nacidos bajo peso (casos y 100 madres de niños normopesos (controles a razón de 2 por cada caso, seleccionadas del registro de nacimientos del área. Se revisaron las historias clínicas obstétricas y se entrevistó a cada paciente para evaluar sus hábitos alimentarios según frecuencia de comidas y grupos de alimentos consumidos. El procesamiento de la información se realizó utilizando el programa estadístico SPSS versión 15,0. Los resultados se presentaron en tablas con frecuencias absolutas, porcentajes y resultados de las técnicas estadísticas empleadas. Resultados: se comportaron como factores de riesgo: la edad materna, el estado nutricional al inicio del embarazo, la ganancia de peso durante la gestación y la hemoglobina del tercer trimestre. Las mujeres con hábitos alimentarios inestables e insuficientes tuvieron 33 veces más probabilidades de tener neonatos bajo peso y las que refirieron dietas no saludables según grupos de alimentos consumidos tuvieron 9 veces más riesgo. Conclusiones: los factores nutricionales maternos influyeron en la incidencia del bajo peso al nacer, fundamentalmente los hábitos alimentarios inadecuados.

  5. Factores de riesgo de bajo peso al nacer en un hospital cubano, 1997-2000

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    Escobar José Andrés Cabrales

    2002-01-01

    Full Text Available Objetivo. Identificar qué factores de riesgo materno durante el embarazo se asocian a la incidencia de bajo peso al nacer (menos de 2 500 g. Métodos. Se diseñó un estudio de casos y testigos con 764 casos de nacimiento simple con un peso al nacer de menos de 2 500 g, y 1 437 testigos del Hospital Gineco-Obstétrico Provincial de Sancti Spíritus, Cuba, durante el cuatrienio de 1997-2000. Los datos se obtuvieron de historias clínicas, registros de partos y entrevistas personales con las madres. Se realizó un análisis con dos variables y se controlaron los posibles factores de confusión mediante regresión logística dicotómica con el programa SPSS. Se usó un modelo final de múltiples variables para identificar los factores de riesgo maternos asociados con el bajo peso al nacer. Resultados. El análisis con múltiples variables mostró una asociación significativa del bajo peso al nacer con la presencia de anemia en la madre cuando se detectó el embarazo, la sepsis urinaria durante el embarazo, el asma bronquial, un aumento de peso materno menor de 8 kg durante todo el embarazo, el hábito de fumar durante el mismo, antecedentes de bajo peso al nacer en partos anteriores, la presencia de hipertensión arterial desde antes de la gestación y la consulta prenatal extemporánea. Conclusiones. A partir de estos resultados se concluyó que, independientemente de la medida de impacto utilizada, es importante tratar de disminuir los factores de riesgo identificados a fin de reducir la incidencia del bajo peso al nacer en la provincia de Sancti Spíritus.

  6. Factores de riesgo de bajo peso al nacer en un hospital cubano, 1997-2000

    Directory of Open Access Journals (Sweden)

    José Andrés Cabrales Escobar

    2002-09-01

    Full Text Available Objetivo. Identificar qué factores de riesgo materno durante el embarazo se asocian a la incidencia de bajo peso al nacer (menos de 2 500 g. Métodos. Se diseñó un estudio de casos y testigos con 764 casos de nacimiento simple con un peso al nacer de menos de 2 500 g, y 1 437 testigos del Hospital Gineco-Obstétrico Provincial de Sancti Spíritus, Cuba, durante el cuatrienio de 1997-2000. Los datos se obtuvieron de historias clínicas, registros de partos y entrevistas personales con las madres. Se realizó un análisis con dos variables y se controlaron los posibles factores de confusión mediante regresión logística dicotómica con el programa SPSS. Se usó un modelo final de múltiples variables para identificar los factores de riesgo maternos asociados con el bajo peso al nacer. Resultados. El análisis con múltiples variables mostró una asociación significativa del bajo peso al nacer con la presencia de anemia en la madre cuando se detectó el embarazo, la sepsis urinaria durante el embarazo, el asma bronquial, un aumento de peso materno menor de 8 kg durante todo el embarazo, el hábito de fumar durante el mismo, antecedentes de bajo peso al nacer en partos anteriores, la presencia de hipertensión arterial desde antes de la gestación y la consulta prenatal extemporánea. Conclusiones. A partir de estos resultados se concluyó que, independientemente de la medida de impacto utilizada, es importante tratar de disminuir los factores de riesgo identificados a fin de reducir la incidencia del bajo peso al nacer en la provincia de Sancti Spíritus.

  7. Short-term toxicity of polystryrene microplastics on mysid shrimps Neomysis japonica

    Science.gov (United States)

    Wang, Mingxin; Wang, Xiao; Luo, Xianxiang; Zheng, Hao

    2017-04-01

    Plastic debris especially microplastics (MPs) have become a global concern for the aggravating pollution in the oceans. In this study, the physico-chemical properties of fluorescently labeled polystyrene (PS) beads and the effects of PS-MPs on the survival of mysid shrimps (Neomysis japonica) were investigated. PS-MPs were identified to have spherical shape, uniform size and stable green fluorescence. The results showed that PS beads had little effects on the mortality of shrimps under a short-term (72 h) exposure with concentrations of 50 μg L-1 and 500 μg L-1. However, PS-MPs had severe short-term toxicity on the survival of mysid shrimps, resulting in 30% mortality especially in a 72 h exposure with the higher concentration of 1000 μg L-1. These findings provide new insights into the toxic effects of MPs on marine invertebrates.

  8. Micro-PIXE study of whole otolith of Anguilla japonica at elver stage

    International Nuclear Information System (INIS)

    Zheng, Y.; Guo, H.; Wei, K.; Tang, W.; Satoh, T.; Ohkubo, T.; Yamazaki, A.; Takano, K.; Kamiya, T.; Shen, H.; Yang, M.; Mi, Y.

    2010-01-01

    Strontium and calcium contents, within the otolith of Anguilla japonica, were measured by external micro-PIXE. According to the measured metamorphic checks, each otolith was divided into three stages. Comparing with the Sr:Ca ratios in stage 2, the ratios in stage 1 had two different trends. Among these fish, it may reflect their maternal condition was not the same. The ratios in stage 3 which was corresponding to the estuarine habitat were smaller than that in any other stage which was corresponding to the ocean habitat in each otolith. Suggested by our results, the eels from the spawning site may separate into two groups when they are near to the south of Taiwan, and then move to the different estuaries in China. It could be proposed that, in general, the migration direction is from south to north along the east coast in China.

  9. Micro-PIXE study of whole otolith of Anguilla japonica at elver stage

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Y. [Applied Ion Beam Physics Laboratory, Institute of Modern Physics, Fudan University, Shanghai 200433 (China); Guo, H.; Wei, K.; Tang, W. [Institute of Life Science, Shanghai Fisheries University, Shanghai 200090 (China); Satoh, T.; Ohkubo, T.; Yamazaki, A.; Takano, K.; Kamiya, T. [Takasaki Advanced Radiation Research Institute, JAEA, Gunma 370-1292 (Japan); Shen, H., E-mail: haoshen@fudan.edu.c [Applied Ion Beam Physics Laboratory, Institute of Modern Physics, Fudan University, Shanghai 200433 (China); Yang, M.; Mi, Y. [Applied Ion Beam Physics Laboratory, Institute of Modern Physics, Fudan University, Shanghai 200433 (China)

    2010-06-15

    Strontium and calcium contents, within the otolith of Anguilla japonica, were measured by external micro-PIXE. According to the measured metamorphic checks, each otolith was divided into three stages. Comparing with the Sr:Ca ratios in stage 2, the ratios in stage 1 had two different trends. Among these fish, it may reflect their maternal condition was not the same. The ratios in stage 3 which was corresponding to the estuarine habitat were smaller than that in any other stage which was corresponding to the ocean habitat in each otolith. Suggested by our results, the eels from the spawning site may separate into two groups when they are near to the south of Taiwan, and then move to the different estuaries in China. It could be proposed that, in general, the migration direction is from south to north along the east coast in China.

  10. Alnuheptanoid B: A new Cyclic Diarylheptanoid from Alnus japonica Stem Bark

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    Sabrin R.M. Ibrahim

    2016-05-01

    Full Text Available A new cyclic diarylheptanoid namely alnuheptanoid B (3, along with four known cyclic diarylheptanoids: myricanone (1, (+-S-myricanol (2, myricanone 5-O- b -D-glucopyranoside (4, and (+-S-myricanol 5-O- b -D-glucopyranoside (5 were isolated from Alnus japonica Steud (Betulaceae stem bark. Their structures were determined by spectroscopic analyses, including UV, IR, 1D ( 1H and 13C, 2D (COSY, HMQC, and HMBC, and HRESIMS, as well as optical rotation measurement. Compounds 1, 2, 4, and 5 are reported for the first time from the plant. All isolated compounds 1-5 were tested for their antioxidant and anti-inflammatory activities using DPPH assay and carrageenin induced rat paw edema model, respectively. They displayed significant antioxidant activity in relation to propyl gallate (positive control. Compound 2 demonstrated anti-inflammatory effect at a dose 10 mg/kg.

  11. Breeding of Hangtian 36-an early-maturing variety of japonica rice by space mutation technique

    International Nuclear Information System (INIS)

    Wang Junmin; Luo Rongting; Bao Genliang; Zhang Mingxian; Xu Jianlong; Wu Wei

    2007-01-01

    Hangtian 36; an early-maturing late Japonica rice variety with high grain quality and disease resistance was developed from the mutagenesis progeny of Bing 1067 carried by the recoverable satellite. Hangtian 36 has advantages over the original variety Bing 1067 in mature duration, grain quality and blast resistance. It has strong tolerant ability to late sow, varying from sowing date of single cropping to the end of July. So it is an ideal variety suitable for the use of emergency situation, such as in the natural disaster year resulting from typhoon. This variety was officially registered by Zhejiang Committee on Variety Registration in 2006, and is adaptable in the region of Zhejiang province and the Yangtze River delta. The successful breeding of Hangtian 36 showed that space mutation is an effective method of simultaneous improvement for multiple traits of rice. (authors)

  12. Cloning of genes and enzymatic characterizations of novel dioscorin isoforms from Dioscorea japonica.

    Science.gov (United States)

    Xue, You-Lin; Miyakawa, Takuya; Sawano, Yoriko; Tanokura, Masaru

    2012-02-01

    Dioscorin, the major tuber storage protein of yam, has been shown to possess carbonic anhydrase, trypsin inhibitor, dehydroascorbate reductase, and monodehydroascorbate reductase activities. In the present study, dioscorin from Dioscorea japonica was confirmed as a glycoprotein using the enhanced concanavalin A-peroxidase staining method, and the protein was shown to have both N- and O-glycans. Following the gene cloning, four full-length isoforms of dioscorin were expressed in Escherichia coli and purified by affinity purification and anion-exchange chromatography for structural and biochemical experiments. It was clearly observed that the recombinant dioscorins had carbonic anhydrase, trypsin inhibitor, dehydroascorbate reductase, and monodehydroascorbate reductase activities. However, the dehydroascorbate reductase and monodehydroascorbate reductase activities were markedly decreased in recombinant dioscorins compared with native dioscorin. The decreased activities were closely related to the loss of the glycosylation from the protein. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  13. Herbological studies of Coptidis rhizoma (Part 2): on the old Japanese name of Coptis japonica makino.

    Science.gov (United States)

    Kawamoto, M; Mikage, M

    1999-01-01

    The rhizome of Coptis plant of the family ranunculaceae has been used in traditional Chinese medicine as a crude drug (Huanglian in present Chinese). In Japan, the rhizome of Coptis japonica Makino (the botanical name of which is "Ohren" in modern Japanese) has been utilized as a crude drug since the Nara era. The Japanese name in those days was "Kakumakusa based on descriptions in old books written in the Heian era. It changed to "Kakumikusa" early in the Edo era, and then changed again to Kakumakusa later in the Edo era. Through this herbological study, it was conjectured as follows: The resemblance between Chinese characters (or MA) and (or MI) in cursive style caused mis-copying of the character. After the "Honzo-wamyo, written in the Heian era, was revised in the late Edo era, and on which the name was accurately written as Kakumakusa, the mistake was corrected.

  14. Evaluation of Marine Algae Wakame (Undaria pinnatifida and Kombu (Laminaria digitata japonica as Food Supplements

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    Luciana Vallorani

    2004-01-01

    Full Text Available Crude proteins and their amino acid composition, -carotene, vitamins B1, B2, B6, niacin and minerals were determined in two edible brown marine algae (Phaeophyceae, Wakame (Undaria pinnatifida and Kombu (Laminaria digitata japonica. The amino acid scores for five key essential amino acids, frequently deficient in mixed human diet, and essential amino acid index were calculated. The results have shown the presence of all essential amino acids. The values of essential amino acid ratios of analysed algae exceed the ratios of reference proteins suggested by FAO/WHO/UNU, except for tryptophan, the first limiting amino acid in both analysed algae. Iodine, the most important component of sea vegetables is present in high amounts as well as the vitamins B1, B2, B6, niacin and β-carotene. The content of minerals was found high, while the presence of heavy metals was negligible.

  15. Influence of Sulfur Fumigation on the Chemical Constituents and Antioxidant Activity of Buds of Lonicera japonica

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    Ai-Li Guo

    2014-10-01

    Full Text Available Lonicera japonica flos is widely used as a pharmaceutical resource and a commonly-employed ingredient in healthy food, soft beverages and cosmetics in China. Sometimes, sulfur fumigation is used during post-harvest handling. In this study, a comprehensive comparison of the chemical profile between sun-dried and sulfur-fumigated samples was conducted by HPLC fingerprints and simultaneous quantification of nine constituents, including secologanic acid, along with another eight usually-analyzed markers. Secologanic acid was destroyed, and its sulfonates were generated, whereas caffeoylquinic acids were protected from being oxidized. The residual sulfur dioxide in sulfur-fumigated samples was significantly higher than that in sun-dried samples, which might increase the potential incidence of toxicity to humans. Meanwhile, compared with sun-dried samples, sulfur-fumigated samples have significantly stronger antioxidant activity, which could be attributed to the joint effect of protected phenolic acids and flavonoids, as well as newly-generated iridoid sulfonates.

  16. Evaluating the Effect of a Novel Molluscicide in the Endemic Schistosomiasis Japonica Area of China

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    Jing Xia

    2014-10-01

    Full Text Available Oncomelania hupensis is the sole intermediate host snail of Schistosoma japonicum in China. Snail control by molluscicide remains one of the most effective measures of schistosomiasis japonica control. A 50% wettable powder of niclosamide ethanolamine salt (WPN is widely used for snail control in China. However, WPN is costly and toxic to fish. A novel molluscicide named LDS, the salt of quinoid-2′, 5-dichloro-4′-nitrosalicylanilide from niclosamide, has been developed. To evaluate the effects of large-scale field application of LDS on field snail control, tests were conducted in 15 counties of Hubei Province, China. Active adult snails, were immersed in 0.2, 0.4, and 0.6 g/m3 of 10% LDS, 1.0 g/m3 of 50% WPN was used as the molluscicide control, and then the mortality rates of snails were investigated after 1, 2, and 3 days. In addition, four active concentrations of 10% LDS (0.4, 0.6, 0.8 and 1.0 g/m2 were applied by spraying and powdering in the field. 1.0 g/m2 of 50% WPN was used as the molluscicide control, and then the mortality rates of snails were observed after 1, 3, and 7 days. The results indicated that 0.4 g/m3 LDS applied by the immersion or 0.6 g/m2 LDS applied by spraying and powdering achieved the same molluscicidal effect as that of WPN, regardless of exposure time. By using different methods, the snail mortality rates in the molluscicide groups were related to exposure time and concentration, respectively. LDS costs less than WPN; thus, LDS is suitable and applicable for use as a molluscicide in schistosomiasis japonica epidemic areas.

  17. Wound repair and anti-inflammatory potential of Lonicera japonica in excision wound-induced rats.

    Science.gov (United States)

    Chen, Wei-Cheng; Liou, Shorong-Shii; Tzeng, Thing-Fong; Lee, Shiow-Ling; Liu, I-Min

    2012-11-23

    Lonicera japonica Thunb. (Caprifoliaceae), a widely used traditional Chinese medicinal plant, is used to treat some infectious diseases and it may have uses as a healthy food and applications in cosmetics and as an ornamental groundcover. The ethanol extract of the flowering aerial parts of L. japonica (LJEE) was investigated for its healing efficiency in a rat excision wound model. Excision wounds were inflicted upon three groups of eight rats each. Healing was assessed by the rate of wound contraction in skin wound sites in rats treated with simple ointment base, 10% (w/w) LJEE ointment, or the reference standard drug, 0.2% (w/w) nitrofurazone ointment. The effects of LJEE on the contents of hydroxyproline and hexosamine during healing were estimated. The antimicrobial activity of LJEE against microorganisms was also assessed. The in vivo anti-inflammatory activity of LJEE was investigated to understand the mechanism of wound healing. LJEE exhibited significant antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans, and Candida tropicalis. The ointment formulation prepared with 10% (w/w) LJEE exhibited potent wound healing capacity as evidenced by the wound contraction in the excision wound model. The contents of hydroxyproline and hexosamine also correlated with the observed healing pattern. These findings were supported by the histopathological characteristics of healed wound sections, as greater tissue regeneration, more fibroblasts, and angiogenesis were observed in the 10% (w/w) LJEE ointment-treated group. The results also indicated that LJEE possesses potent anti-inflammatory activity, as it enhanced the production of anti-inflammatory cytokines that suppress proinflammatory cytokine production. The results suggest that the antimicrobial and anti-inflammatory activities of LJEE act synergistically to accelerate wound repair.

  18. Variation in Yield and Physicochemical Quality Traits among Mutants of Japonica Rice Cultivar Wuyujing 3

    Directory of Open Access Journals (Sweden)

    Jose Daniel Abacar

    2016-01-01

    Full Text Available To select elite germplasms, 112 mutants derived from japonica rice cultivar Wuyujing 3 were evaluated. The yield components such as panicle number per square meter, grain number per panicle, and grain weight were measured. The quality traits such as percentage of chalky grains (PCG, brown rice yield (BRY, milled rice yield (MRY, degree of milling (DM, amylose content (AC, protein content (PC, and relationships among traits were inverstigated. Results showed that grain yield ranged from 2.15 to 12.49 t/hm2 with a mean of 6.4 t/hm2 and number of grains per square meter contributed for 94.64% in grain yield variation. For quality traits, all rice mutants had short size (grain length ≤ 5.5 mm and bold shape (grain length to width ratio = 1.10–2.00. Most of rice mutants (87.5% had PCG values below 20%. All mutants had MRY values above 50%, AC values below 20%, and PC values below 10%. Percentage of chalky grains was significantly negatively correlated with MRY and positively correlated with DM. BRY and MRY were significantly negatively correlated with DM. PC was significantly and positively correlated with MRY and negatively correlated with DM, while AC had no significant correlation with these quality traits. It was concluded that there were 25 rice mutants which fulfilled the major requirements of Jiangsu standard japonica rice such as low percentage of chalky grains, low amylose content, optimal protein content, and which could be used as elite germplasms. Thus the mutants identified may lead to significant progress in improvement of rice quality.

  19. Bajo Peso al Nacer. Factores de riesgo y calidad de la atención Prenatal. Buenaventura. 2008-2009

    Directory of Open Access Journals (Sweden)

    Luis Enrique Gallardo-Ibarra

    2012-01-01

    Full Text Available Se realizó estudio descriptivo sobre Bajo Peso al Nacer, Área de Salud Buenaventura, bienio 2008-2009, sobre factores de riesgo y calidad de la atención médica recibida por las gestantes que aportaron recién nacidos bajo peso. Los datos estadísticos se obtuvieron mediante revisión de historias clínicas familiares, obstétricas y hospitalarias. Se emplearon métodos estadísticos histórico lógico, análisis y síntesis e inducción-deducción. Se obtuvo predominio del CIUR como variedad de Bajo Peso al Nacer, mostraron asociación con el bajo peso al nacer la valoración nutricional Bajo Peso materna al inicio de la gestación, insuficiente ganancia de peso, ingreso percápita bajo, malas condiciones de vivienda, antecedentes de dos o más abortos, multigestación, período intergenésico corto, HTA y sepsis cervicovaginal. La Atención Prenatal fue deficiente. No existió relación entre grupos de edades menores de 20 o mayor de 35 años y hábitos tóxicos con la aparición del bajo peso al nacer.

  20. Lodging Resistance of Japonica Rice (Oryza Sativa L.): Morphological and Anatomical Traits due to top-Dressing Nitrogen Application Rates.

    Science.gov (United States)

    Zhang, Wujun; Wu, Longmei; Wu, Xiaoran; Ding, Yanfeng; Li, Ganghua; Li, Jingyong; Weng, Fei; Liu, Zhenghui; Tang, She; Ding, Chengqiang; Wang, Shaohua

    2016-12-01

    Lodging in rice production often limits grain yield and quality by breaking or bending stems. Excessive nitrogen (N) fertilizer rates are the cause of poor lodging resistance in rice, but little is known about the effect of top-dressing N application rates on the mechanical strength of japonica rice plants, especially how the anatomical structure in culms is affected by N. In this study, field experiments on two japonica rice varieties with three top-dressing N application rates, 0 kg N ha(-1) (LN), 135 kg N ha(-1) (MN), and 270 kg N ha(-1) (HN) as urea, were conducted. Wuyunjing23, a lodging-resistant japonica rice cultivar and W3668, a lodging-susceptible japonica rice cultivar were used. The lodging index, breaking strength, morphological and anatomical traits in culms were measured in this study. The visual lodging rate in japonica rice differed remarkably between genotypes and top-dressing N treatments. The higher lodging index of rice plants was primarily attributed to the weak breaking strength of the lower internodes. The longer elongated basal internodes were responsible for higher plant height and a higher lodging index. Correlation analysis showed that breaking strength was significantly and positively correlated with the thickness of the mechanical tissue but was significantly and negatively correlated with the inner diameter of the major axis (b2). With increasing top-dressing N rates, the sclerenchyma cells of the mechanical tissues and the vascular bundles of the Wuyunjing23 cultivar varied little. The plant height, inner diameter of the minor axis (a2) and b2 increased significantly, but the area of the large vascular bundle (ALVB) and the area of the small vascular bundle (ASVB) decreased significantly and resulted in lower stem strength and a higher lodging index under higher top-dressing N conditions. The culm diameter of the W3668 cultivar increased slightly with no significant difference, and the sclerenchyma cells in the mechanical

  1. Comparison of the uptake and assimilation of ammonium and nitrate in Indica and Japonica rice plants using the tracer 15N method

    International Nuclear Information System (INIS)

    Ta, T.C.; Ohira, Koji

    1982-01-01

    The uptake of 15 N-labeled ammonium and nitrate and their assimilation in 4-week-old Indica and Japonica rice plants were studied during 24 hr exposure to 2 mM solutions of ( 15 NH 4 ) 2 SO 4 and/or Na 15 NO 3 . Although there was no clear difference in the uptake and assimilation of 15 NH 4+ , significant differences in the uptake and assimilation of 15 NO 3- by both varieties were observed. When NH 4+ or NO 3- was supplied exclusively, the Indica rice plants absorbed the latter more effectively than the Japonica. In addition, despite a preference for uptake of NH 4+ rather than NO 3- as shown by both varieties when both forms were supplied together, the relative amounts of NO 3- uptake by the Indica were higher than those of Japonica. On the other hand, the Indica rice plants reduced the absorbed 15 NO 3- more rapidly than the Japonica. The incorporation 15 NO 3- into the ethanol insoluble nitrogen fraction of the Indica rice also exceeded that of the Japonica. These results suggest that the Indica has the ability to utilize NO 3- as a nitrogen source more effectively than the Japonica rice plants. (author)

  2. Preventive effect of Dioscorea japonica on squamous cell carcinoma of mouse skin involving down-regulation of prostaglandin E2 synthetic pathway.

    Science.gov (United States)

    Tsukayama, Izumi; Toda, Keisuke; Takeda, Yasunori; Mega, Takuto; Tanaka, Mitsuki; Kawakami, Yuki; Takahashi, Yoshitaka; Kimoto, Masumi; Yamamoto, Kei; Miki, Yoshimi; Murakami, Makoto; Suzuki-Yamamoto, Toshiko

    2018-03-01

    Hyperproduced prostaglandin E 2 by cyclooxygenase-2 and microsomal prostaglandin E synthase-1 evokes several pathophysiological responses such as inflammation and carcinogenesis. Our recent study demonstrated that Dioscorea japonica extract suppressed the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 and induced apoptosis in lung carcinoma A549 cells. In the present study, we investigated the effects of Dioscorea japonica on squamous cell carcinoma of mouse skin. Dioscorea japonica feeding and Dioscorea japonica extract topical application suppressed the expression of cyclooxygenase-2, microsomal prostaglandin E synthase-1, interleukin-1β and interleukin-6 and inhibited tumor formation, hyperplasia and inflammatory cell infiltration. Immunohistochemical analyses showed the immunoreactivities of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in tumor keratinocytes and stronger immunoreactivities of cyclooxygenase-2 and hematopoietic prostaglandin D synthase in epidermal dendritic cells (Langerhans cells). Treatment with Dioscorea japonica decreased the immunoreactivity of cyclooxygenase-2 and microsomal prostaglandin E synthase-1. These results indicate that Dioscorea japonica may have inhibitory effects on inflammation and carcinogenesis via suppression of the prostaglandin E 2 synthetic pathway.

  3. Algas epífitas de Bajo Pepito, Isla Mujeres, Quintana Roo, México

    Directory of Open Access Journals (Sweden)

    L.I Quan-Young

    2006-06-01

    Full Text Available Epiphytic algae from Bajo Pepito, Isla Mujeres, Quintana Roo, México. A total of 96 epiphytic algae species were identified from Bajo Pepito, Quintana Roo, México. 60.4% (58 belonged to the Rhodophyta, 19.79% (19 to the Phaeophyta, 16.6% (16 to the Chlorophyta and 3.1% (3 to the Cyanophyta; 49 species (50.5% were found only in one month, while Heterosiphonia crispella was found in all of the sampled months. That species provided the largest contribution to the biomass of epiphytes. During January we registered the greater biommass and richness of epiphytes species, coincidently with high values of host species cover and rainfall. Rev. Biol. Trop. 54(2: 317-328. Epub 2006 Jun 01.

  4. Estudio de una Cronosecuencia de Suelos en el Bajo Cauca (Antioquia

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    Jaramillo J. Daniel F.

    1996-09-01

    Full Text Available En la región del Bajo Cauca, Antioquia, se estudiaron tres terrazas, a diferentes alturas, las cuales poseen diferencias apreciables entre la evolución de sus respectivos suelos. La terraza de mayor altura presentó los suelos más evolucionados: Typic Paleudult; la de mediana altura presentó suelos de incipiente evolución, clasificados como Typic Dystropept y la de menor altitud presentó suelos recientes, con muy bajo grado de evolución: Tropic Fluvaquent. Las diferencias en evolución observadas entre los suelos se deben a los largos intervalos de tiempo transcurridos entre la formación de los tres niveles de las terrazas. La fertilidad natural de los suelos desarrollados sobre las terrazas alta y media se puede calificar como baja a muy baja; por su parte, la terraza a menor altura posee alta fertilidad.

  5. DESARROLLO E IMPLEMENTACIÓN DE FOTOCONTROL TEMPORIZADO CON TECNOLOGÍA DE BAJO CONSUMO

    Directory of Open Access Journals (Sweden)

    Fredy Hernán Martínez Sarmiento

    2013-09-01

    Full Text Available En este trabajo se presenta el desarrollo e implementación de un fotocontrol con tecnología de bajo consumo desarrollado con un microcontrolador, el cual controla variables de tensión, luminosidad  y sincronización con la red, por medio de  una salida con modo PWM producir una salida de Vdc en el relé. El microcontrolador realiza un  muestreo análogo de voltaje sobre la onda sinusoidal y se hace un filtro de promedio móvil para eliminar ruido de la onda.La temporización se realiza cuando el contacto del relé está cerrado y el tiempo a temporizar es programado por el usuario el cual lo requiera, el fotocontrol presenta un modo de ultra bajo consumo en el microcontrolador con un supercap.

  6. Habilidades pre-escolares de los niños nacidos prematuramente y con bajo peso

    OpenAIRE

    Sasha A. Martínez-Espiet; Irene Sumaza Laborde; Leila Crespo Fernández; Maribel Campos Rivera; Frances Boulon Díaz

    2016-01-01

    RESUMEN: El objetivo de este estudio fue conocer el nivel de desarrollo en la etapa pre-escolar de un grupo de niños nacidos prematuramente y con bajo peso. Se evaluó un grupo 20 niños de cuatro años nacidos prematuramente y con bajo peso y un grupo de 20 niños nacidos luego de gestación completada y peso deseado utilizando la prueba de integración visual-motora Beery-Buktenica. Además, se administró el cuestionario del desarrollo Ages and Stages (ASQ-3) a las 40 madres. El análisis estadísti...

  7. Los exilios de católicos y arrianos bajo Leovigildo y Recaredo

    Directory of Open Access Journals (Sweden)

    Vallejo Girvés, Margarita

    2003-06-01

    Full Text Available In this paper we try to analyse the various topics of the exile that have been applied by the visigothic kings Leovigild and Reccared. It is known that most part of the individuals that had suffered this exile were ecclesiasticals, catholics under Leovigild and arians during Reccared, but all of them were of germanic origin.

    En este artículo se analizan los diversos tipos de exilio que aplicaron los monarcas visigodos Leovigildo y Recaredo. Se da la circunstancia de que la mayoría de ellos fueron eclesiásticos y que bajo Leovigildo lo sufrieron singularmente los católicos y los arríanos bajo Recaredo, pero en ambos casos todos los condenados son de origen germánico.

  8. Bajo rendimiento escolar: una perspectiva desde el desarrollo del sistema nervioso

    Directory of Open Access Journals (Sweden)

    K. Shapiro Bruce, MD.

    2011-03-01

    Full Text Available El bajo rendimiento escolar es un problema frecuente y tiene múltiples causas; las alteraciones que lo caracterizan, se expresan fundamentalmente en las áreas de funcionamiento cognitivo, académico y conductual. El bajo rendimiento escolar es una vía final común de diferentes trastornos, etiologías y mecanismos. Es habitual la presencia de múltiples alteraciones, porque la disfunción cerebral en la niñez generalmente afecta a muchas funciones. Consecuente con lo anterior, los programas de manejo deben ser individualizados, comprensivos e incorporar aspectos del niño en particular, la escuela y la familia. La planificación del tratamiento incluye, educación y entrenamiento de los padres, adecuaciones académicas, técnicas para mantener la autoestima y un enfoque psicofarmacológico. Es necesario monitorear en forma continua los programas de manejo especialmente para detectar comorbilidades importantes que puedan emerger, para realizar modificaciones que se adecuen a los cambios en las demandas académicas y sociales a las diferentes edades del niño y para proveer de información actualizada. Las consecuencias que tenga el bajo rendimiento escolar para el niño dependerán en medida importante de las alteraciones subyacentes. El personal de salud tiene múltiples roles en la prevención, detección, diagnóstico y manejo del niño con bajo rendimiento escolar.

  9. Actualización en el manejo del colesterol hdl bajo

    Directory of Open Access Journals (Sweden)

    Rigotti R. Attilio, Dr.

    2012-11-01

    Full Text Available Los niveles plasmáticos bajos de colesterol transportado en las lipoproteínas de alta densidad (HDL constituyen un elemento independiente de riesgo cardiovascular ateroesclerótico. Algunos protocolos clínicos enfocados en el manejo farmacológico de los niveles bajos de colesterol HDL en pacientes de alto riesgo cardiovascular han mostrado resultados favorables. Por otro lado, existen nuevas formulaciones de la niacina, el agente farmacológico disponible más efectivo para el manejo del colesterol HDL bajo, y se están desarrollando nuevos fármacos más potentes para aumentar los niveles plasmáticos de colesterol HDL. En el futuro, nuevos medicamentos que modulen el metabolismo de HDL y demuestren beneficio sobre el control del riesgo cardiovascular ateroesclerótico, podrían mejorar aún más el manejo actual de esta condición patológica que se basa esencialmente en el uso de estatinas.

  10. Perfil cognitivo de niños con rendimientos bajos en comprensión lectora

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    Lorena Canet-Juric

    2013-10-01

    Full Text Available La mayoría de los niños con dificultades en la comprensión lectora poseen rendimientos inferiores en un conjunto de procesos cognitivos. El objetivo del presente estudio es establecer un perfil cognitivo asociado al bajo rendimiento en comprensión lectora en relación a la habilidad de monitoreo, a la producción de inferencias, al vocabulario, a la amplitud de memoria de trabajo y a la capacidad inhibitoria, en niños hispanoparlantes. Para ello se administraron tareas correspondientes a dichos procesos en niños con altos y bajos rendimientos en comprensión lectora. Los resultados mostraron que si bien los niños con mala comprensión presentan bajos rendimientos en estas habilidades, no puede establecerse un perfil consistente ya que hay niños con problemas en comprensión que presentan normal o superior rendimiento en algunas de estas habilidades cognitivas.

  11. Effects of Swertia japonica extract and its main compound swertiamarin on gastric emptying and gastrointestinal motility in mice.

    Science.gov (United States)

    Kimura, Yoshiyuki; Sumiyoshi, Maho

    2011-09-01

    The Swertia japonica is used clinically as a remedy for gastrointestinal symptoms in Japan. We examined the effects of a S. japonica and swertiamarin on gastric emptying and gastrointestinal motility in atropine-, dopamine-, and 5-hydroxytryptamine (5-HT)-treated mice. All three preparations inhibited reductions in gastric emptying and gastrointestinal motility induced by dopamine (1mg/kg, intraperitoneal injection, ip). Neither the powder, swertiamarin, nor itopride had any effect on the reductions in gastric emptying and gastrointestinal motility caused by 5-HT (4 mg/kg, ip). These findings suggest that the powder and swertiamarin stimulate gastric emptying and gastrointestinal motility by inhibiting the dopamine D(2) receptor. Copyright © 2011 Elsevier B.V. All rights reserved.

  12. Effect of temperature of 32P uptake and translocation in varieties and a cross of japonica and indica rice

    International Nuclear Information System (INIS)

    Reddy, P.R.; Madhusudhan Rao, L.

    1976-01-01

    Three genotypes of rice, one in each of japonica, indica, and a japonica-indica cross were grown in half-Hoagland culture solution. 32 P (40 μCi/I) was added to each of these culture jars and placed at 10,20,30 and 40degC in growth chamber for a period of 1,5 and 10 h at each temperature. The actual uptake of 32 P in plant was very low at 10 and 20degC; moderately low at 40degC and very low at 30degC in japonica and the cross. The uptake was higher at 30 and 40degC in indica. This indicates their ability in uptake of fertilizer at these temperatures. The percentage of absorbed ions decreased at 10 and 20degC and increased at 30 and 40degC with increased duration in all the genotypes. The actual uptake of ions was in proportion to the absorbed ions at higher temperatures and inversely proportional at lower temperatures. The percentage of translocation of 32 P to shoot increased with time between 20 and 50degC in indica, whereas it was high only at 30 and 40degC in japonica and the cross. The translocation was low at 10degC in all the genotypes. The uptake of 32 P at different concentrations under different diurnal temperatures (20/10, 30/20 and 40/30degC) was also studied. (author)

  13. Overcoming interspecific incompatibility in the cross Brassica campestris ssp. japonica x Brassica oleracea var. botrytis using irradiated mentor pollen page

    International Nuclear Information System (INIS)

    Sarla, N.

    1988-01-01

    The cross B. campestris ssp. japonica x B. oleracea var. botrytis fails due to incompatibility barrier at the stigma. To realize this cross, irradiated compatible pollen (mentor pollen) was used before the incompatible pollination. The presence of mentor pollen stimulated the incompatible pollen to germinate and effect fertilization and seed set. One hybrid was thus obtained. Most of the seeds were inviable. Of the 5 plants raised one was a hybrid and 4 resembled the female parent. 1 tab., 7 refs

  14. Measurement of injected Sr in the stem of a Japanese cedar (Cryptomeria japonica D. Don) using PIXE

    International Nuclear Information System (INIS)

    Katayama, Y.; Aoki, T.; Ko, S.; Yoshida, K.

    1999-01-01

    Distribution profiles of Sr injected into the stem of a Japanese cedar (Cryptomeria japonica D. Don) were determined using PIXE. The Sr injected into the middle of the sapwood of the cedar stem moved upwards easily along the grain. The Sr in the stem moved in a radial direction in the sapwood section (assumed to be through the ray) and there was almost no tangential movement. (author)

  15. A structure-activity relationship study on antiosteoclastogenesis effect of triterpenoids from the leaves of loquat (Eriobotrya japonica).

    Science.gov (United States)

    Tan, Hui; Ashour, Ahmed; Katakura, Yoshinori; Shimizu, Kuniyoshi

    2015-04-15

    Our previous results elucidated that the leaves of Eriobotrya japonica possessed the potential to suppress ovariectomy-induced bone mineral density deterioration, and ursolic acid, the major bioactive component in these leaves, suppressed the osteoclast differentiation. The aim of this study was to discover more candidates for development of novel antiosteoclastogenesis agents from the leaves of E. japonica. Phytochemical analysis following a cell-based osteoclastic tartrate-resistant acid phosphatase (TRAP) activity assay revealed 11 more compounds with a potent antiosteoclastogenesis effect. The potency of ursane-type triterpenoids from the leaves of E. japonica prompted us to investigate the structure-activity relationships underlying their antiosteoclastogenesis. The results revealed that both the hydroxyl group at C-3 and the carboxylic group at C-17 played indispensable roles in the antiosteoclastogenesis activity of ursane-type triterpenoids. The configuration at C-3 (a beta-form of the hydroxyl group) was found to be important for this activity. While introducing a hydroxyl group at C-19 increased the inhibitory activity of ursane-type triterpenoids carrying an alpha-form hydroxyl group at C-3. The bioactivity analyses of ursolic acid and oleanolic acid demonstrated that the antiosteoclastogenesis effect of ursolic acid may be related to different positions of the C-29 and C-30 methyl groups on the E-ring, since oleanolic acid showed limited activity. The addition of a hydroxyl group at C-2 would dramatically improve the inhibition of oleanane-type triterpenoids. Collectively, these findings could provide important clues for the improvement of multi-targeted antiosteoclastogenesis agents from the leaves of E. japonica. Copyright © 2015 Elsevier GmbH. All rights reserved.

  16. De novo assembly and comparison of the ovarian transcriptomes of the common Chinese cuttlefish (Sepiella japonica with different gonadal development

    Directory of Open Access Journals (Sweden)

    Zhenming Lü

    2016-03-01

    Full Text Available The common Chinese cuttlefish (Sepiella japonica has been considered one of the most economically important marine Cephalopod species in East Asia and seed breeding technology has been established for massive aquaculture and stock enhancement. In the present study, we used Illumina HiSeq2000 to sequence, assemble and annotate the transcriptome of the ovary tissues of S. japonica for the first time. A total of 53,116,650 and 53,446,640 reads were obtained from the immature and matured ovaries, respectively (NCBI SRA database SRX1409472 and SRX1409473, and 70,039 contigs (N50 = 1443 bp were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 47,288 contigs show differential expression profile and 793 contigs are highly expressed in the immature ovary, while 38 contigs are highly expressed in the mature ovary with FPKM >100. We hope that the ovarian transcriptome and those stage-enriched transcripts of S. japonica can provide some insight into the understanding of genome-wide transcriptome profile of cuttlefish gonad tissue and give useful information in cuttlefish gonad development. Keywords: Cuttlefish, Gonad development, Transcriptome

  17. [Construction of individual-based ecological model for Scomber japonicas at its early growth stages in East China Sea].

    Science.gov (United States)

    Li, Yue-Song; Chen, Xin-Jun; Yang, Hong

    2012-06-01

    By adopting FVCOM-simulated 3-D physical field and based on the biological processes of chub mackerel (Scomber japonicas) in its early life history from the individual-based biological model, the individual-based ecological model for S. japonicas at its early growth stages in the East China Sea was constructed through coupling the physical field in March-July with the biological model by the method of Lagrange particle tracking. The model constructed could well simulate the transport process and abundance distribution of S. japonicas eggs and larvae. The Taiwan Warm Current, Kuroshio, and Tsushima Strait Warm Current directly affected the transport process and distribution of the eggs and larvae, and indirectly affected the growth and survive of the eggs and larvae through the transport to the nursery grounds with different water temperature and foods. The spawning grounds in southern East China Sea made more contributions to the recruitment to the fishing grounds in northeast East China Sea, but less to the Yangtze estuary and Zhoushan Island. The northwestern and southwestern parts of spawning grounds had strong connectivity with the nursery grounds of Cheju and Tsushima Straits, whereas the northeastern and southeastern parts of the spawning ground had strong connectivity with the nursery grounds of Kyushu and Pacific Ocean.

  18. Expression and function analysis of metallothionein in the testis of stone crab Charybdis japonica exposed to cadmium

    Energy Technology Data Exchange (ETDEWEB)

    Mao Huan [Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058 (China); The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou (China); Tan Fuqing [The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou (China); Wang Dahui [Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058 (China); Zhu Junquan [Faculty of Life Science and Bioengineering, Ningbo University, Zhejiang 315211 (China); Zhou Hong [Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058 (China); Yang Wanxi, E-mail: wxyang@spermlab.org [Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058 (China)

    2012-11-15

    Metallothionein (MT) participates in metallic homeostasis and detoxification in living animals. Previous studies have focused mainly on the functions of MT in vertebrates, but the functions of MT during spermiogenesis in invertebrates remain unclear. In order to investigate the functions of MT during spermiogenesis in the Japanese stone crab (Charybdis japonica), we identified the C. japonica MT complete cDNA sequence from the total RNA of the testis using RT-PCR and RACE. The 587 bp MT cDNA contains: an 80 bp 5 Prime untranslated region, a 333 bp 3 Prime untranslated region, and a 174 bp open reading frame. MT has 57 amino acids including 19 cysteines. The protein alignment between MT sequences of C. japonica and other crabs shows a high similarity and a strong identity in cysteine residues vital for the metal-binding affinity of MT. After the cadmium (Cd) exposure, the testis displays both abnormal morphology and MT mRNA expression both of which indicate a sensitive response of testis MT to Cd. Therefore, we suggest that MT is an excellent biomarker candidate for evaluating Cd pollution.

  19. Can arbuscular mycorrhizal fungi reduce Cd uptake and alleviate Cd toxicity of Lonicera japonica grown in Cd-added soils?

    Science.gov (United States)

    Jiang, Qiu-Yun; Zhuo, Feng; Long, Shi-Hui; Zhao, Hai-Di; Yang, Dan-Jing; Ye, Zhi-Hong; Li, Shao-Shan; Jing, Yuan-Xiao

    2016-02-01

    A greenhouse pot experiment was conducted to study the impact of arbuscular mycorrhizal fungi-Glomus versiforme (Gv) and Rhizophagus intraradices (Ri) on the growth, Cd uptake, antioxidant indices [glutathione reductase (GR), ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT), ascorbate (ASA), glutathione (GSH) and malonaldehyde (MDA)] and phytochelatins (PCs) production of Lonicera japonica in Cd-amended soils. Gv and Ri significantly increased P acquisition, biomass of shoots and roots at all Cd treatments. Gv significantly decreased Cd concentrations in shoots and roots, and Ri also obviously reduced Cd concentrations in shoots but increased Cd concentrations in roots. Meanwhile, activities of CAT, APX and GR, and contents of ASA and PCs were remarkably higher in Gv/Ri-inoculated plants than those of uninoculated plants, but lower MDA and GSH contents in Gv/Ri-inoculated plants were found. In conclusion, Gv and Ri symbiosis alleviated Cd toxicity of L. japonica through the decline of shoot Cd concentrations and the improvement of P nutrition, PCs content and activities of GR, CAT, APX in inoculated plants, and then improved plant growth. The decrease of shoot Cd concentrations in L. japonica inoculated with Gv/Ri would provide a clue for safe production of this plant from Cd-contaminated soils.

  20. Inhibition of Acute Phase Inflammation by Laminaria japonica through Regulation of iNOS-NF-κB Pathway

    Directory of Open Access Journals (Sweden)

    Seong Kyu Park

    2013-01-01

    Full Text Available Laminaria japonica has been frequently used as food supplements in many of the Asian countries and as a drug in traditional oriental medicine. This research investigated the effects of Laminaria japonica extract (LJE on acute phase inflammation in a carrageenan-induced paw edema model, as assessed by histomorphometric and immunohistochemical analyses. The effect of LJE was also evaluated in Raw264.7 cells stimulated with lipopolysaccharide (LPS in the aspect of the inhibition of nitric oxide (NO, prostaglandin E2 (PGE2, and proinflammatory cytokines production. NO, PGE2, tumor necrosis factor (TNF-α, interleukin-1β, and interleukin-6 contents were assayed by ELISA, and inducible NO synthase (iNOS and cyclooxygenase (COX-2 expressions were determined by western blot analyses. In rats, LJE treatment inhibited carrageenan-induced paw edema formation and infiltration of inflammatory cells in H&E staining. LJE treatment prevented the ability of LPS to increase the levels of iNOS and COX-2 protein in a concentration-dependent manner. Consistently, LJE suppressed the production of TNF-α, interleukin-1β, and interleukin-6. Treatment of the cells with LJE caused inhibition of inhibitor of κBα phosphorylation induced by LPS, suggesting LJE repression of nuclear factor-κB activity by LPS. In conclusion, this study shown here may be of help to understand the action mechanism of LJE and the anti-inflammatory use of L. japonica.

  1. Characterize and Gene Expression of Heat Shock Protein 90 in Marine Crab Charybdis japonica following Bisphenol A and 4-Nonylphenol Exposures.

    Science.gov (United States)

    Park, Kiyun; Kwak, Ihn-Sil

    2014-01-01

    Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone important in the maturation of a broad spectrum of protein. In this study, an HSP90 gene was isolated from Asian paddle crab, Charybdis japonica, as a bio-indicator to monitor the marine ecosystem. This work reports the responses of C. japonica HSP90 mRNA expression to cellular stress by endocrine disrupting chemicals (EDCs), such as bisphenol A (BPA) and 4-nonylphenol (NP) using real-time. reverse transcription polymerase chain reaction. The deduced amino acid sequence of HSP90 from C. japonica shared a high degree of homology with their homologues in other species. In a phylogenetic analysis, C. japonica HSP90 is evolutionally related with an ortholog of the other crustacean species. The expression of HSP90 gene was almost distributed in all the examined tissues of the C. japonica crab but expression levels varied among the different body parts of the crabs. We examined HSP90 mRNA expression pattern in C. japonica crabs exposed to EDCs for various exposure times. The expression of HSP90 transcripts was significantly increased in C. japonica crabs exposed to BPA and NP at different concentrations for 12, 24, 48 and 96 hours. The mRNA expression of HSP90 gene was significantly induced in a concentration- and time-dependent manner after BPA or NP exposures for 96 hours. Taken together, expression analysis of Asian paddle crab HSP90 gene provided useful molecular information about crab responses in stress conditions and potential ways to monitor the EDCs stressors in marine environments.

  2. Thalli Growth, Propagule Survival, and Integrated Physiological Response to Nitrogen Stress of Ramalina calicaris var. japonica in Shennongjia Mountain (China

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    Chuan-Hua Wang

    2018-05-01

    Full Text Available In this study, effects of nitrogen (N availability on growth, survival of Ramalina calicaris var. japonica, and whether it respond nitrogen stress in an integrated physiological way was evaluated. Thalli growth and propagule survival, thalli N and phosphorus (P content, and activity of phosphomonoesterase (PME of R. calicaris var. japonica were determined in a field experiment. Its differentiate adsorption in ammonia and nitrate, the activity of glutamine synthetase (GSA and nitrate reductase (NRA also were investigated in a series of indoor experiments. The results showed that N deposition significantly decreased the growth and survival of this lichen, and the N sensitivity threshold was suggested at 6.0 kg N⋅ha-1⋅y-1. When the N deposition increased from 8.59 kg N⋅ha-1⋅y-1 to 14.24, 20.49, 32.99 and 57.99 kg N⋅ha-1⋅y-1, the growth rates of lichen thalli decreased by 26.47, 39.01, 52.18 and 60.3%, respectively; Whereas the survival rate of the lichen propagules decreased from 92.8% of control (0.0 kg N⋅ha-1⋅y-1 to 10.7% of 50.0 kg N⋅ha-1⋅y-1, when they were treated with 0.00, 6.25, 12.5, 25.0, and 50.0 kg N⋅ha-1⋅y-1 deposition. Compared with an adequate adsorption of ammonium N, no nitrate adsorption occurred when thalli was submerged in solution lower than 0.4 mM. Our results also suggested that thalli total nitrogen, N:P ratio increased with N availability, and the activity of PME was significantly correlated with thalli total nitrogen. These all indicated that phosphorus limitation occurred when R. calicaris var. japonica treated with higher nitrogen deposition. Compared with slightly effects of NRA, GSA of R. calicaris var. japonica responded nitrogen availability significantly; In addition, GSA and NRA negatively correlated with thalli growth rate and propagule survival significantly. These results indicated that nitrogen stress do decrease growth and survival of R. calicaris var. japonica, and lichen would be

  3. Moderate Increase in TCO2 Enhances Photosynthesis of Seagrass Zostera japonica, but Not Zostera marina: Implications for Acidification Mitigation

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    Cale A. Miller

    2017-07-01

    Full Text Available Photosynthesis and respiration are vital biological processes that shape the diurnal variability of carbonate chemistry in nearshore waters, presumably ameliorating (daytime or exacerbating (nighttime short-term acidification events, which are expected to increase in severity with ocean acidification (OA. Biogenic habitats such as seagrass beds have the capacity to reduce CO2 concentration and potentially provide refugia from OA. Further, some seagrasses have been shown to increase their photosynthetic rate in response to enriched total CO2 (TCO2. Therefore, the ability of seagrass to mitigate OA may increase as concentrations of TCO2 increase. In this study, we exposed native Zostera marina and non-native Zostera japonica seagrasses from Padilla Bay, WA (USA to various levels of irradiance and TCO2. Our results indicate that the average maximum net photosynthetic rate (Pmax for Z. japonica as a function of irradiance and TCO2 was 3x greater than Z. marina when standardized to chlorophyll (360 ± 33 μmol TCO2 mg chl−1 h−1 and 113 ± 10 μmol TCO2 mg chl−1 h−1, respectively. Additionally, Z. japonica increased its Pmax ~50% when TCO2 increased from ~1,770 to 2,051 μmol TCO2 kg−1. In contrast, Z. marina did not display an increase in Pmax with higher TCO2, possibly due to the variance of photosynthetic rates at saturating irradiance within TCO2 treatments (coefficient of variation: 30–60% relative to the range of TCO2 tested. Our results suggest that Z. japonica can affect the OA mitigation potential of seagrass beds, and its contribution may increase relative to Z. marina as oceanic TCO2 rises. Further, we extended our empirical results to incorporate various biomass to water volume ratios in order to conceptualize how these additional attributes affect changes in carbonate chemistry. Estimates show that the change in TCO2 via photosynthetic carbon uptake as modeled in this study can produce positive diurnal changes in pH and

  4. Preparation and analysis of carbonization products from Sugi (Cryptomeria japonica D. Don) wood; Sugi (Cryptomeria japonica D. Don) zai no tanka seiseibutsu no chosei to bunseki

    Energy Technology Data Exchange (ETDEWEB)

    Matsui, T.; Matsushita, Y.; Sugamoto, K.; Tokuda, Y. [Miyazaki University, Miyazaki (Japan). Faculty of Engineering; Kodama, K.; Nakata, K.; Oda, M.; Yamauchi, H. [Miyazaki Prefectural Institute of Industrial Technology, Miyazaki (Japan)

    2000-01-10

    The material balance of carbonization products (wood-gas, wood-vinegar, wood-tar and charcoal) prepared from the sapwood and the heartwood of Sugi (Cryptomeria japonica D. Don) at various temperatures was determined. The evolution of both carbon monoxide and carbon dioxide mainly occurred at the carbonization temperature below 600 degree C and that of hydrogen and methane at 600-800 degree C. Total yield of the wood-vinegar and the wood-tar was almost constant at 500-800 degree C. On the other hand, the yield of the charcoal decreased because of the evolution of the wood gas with an increase in carbonization temperature. The wood-vinegars prepared at the temperature over 400 degree C were found to consist of carboxylic acids such as acetic acid and propionic acid, methanol, acetone, furans, alkylphenols, guaiacols, cyclotene and maltol by capillary gas chromatography. Since the constituent varieties and contents of the wood-vinegars prepared at both 400 degree C and 800 degree C were very similar each other, the wood-vinegar of Sugi wood chiefly produced below 400 degree C. FT-IR spectra of the charcoals showed the generation of carbonyl and olefin groups at 300-400 degree C and then the formation of aromatic rings along with the disappearance of carbonyl groups over 600 degree C. The production of radical species in the charcoals carbonized at 300-600 degree C was observed by ESR, on the contrary, the charcoals carbonized over 700 degree C were inactive. The specific surface area and the pore volume of the charcoal of Sugi sapwood increased with an increase in carbonization temperature. The pH of charcoal-dispersed aqueous solution increased with an increase in carbonization temperature. The high adsorptive activity of the charcoal carbonized at 400 degree C for ammonia gas and that at 800 degree C for hydrogen sulfide gas seemed to be dependent on acidic and basic properties of the charcoals, respectively. (author)

  5. Condiciones de trabajo materno y bajo peso al nacer en la Ciudad de México

    Directory of Open Access Journals (Sweden)

    PRUDENCIA CERÓN-MIRELES

    1997-01-01

    Full Text Available Objetivo. Estudiar la asociación entre condiciones de trabajo materno y bajo peso al nacer en la Ciudad de México. Material y métodos. Se encuestaron 2 623 trabajadoras que atendieron sus partos en tres grandes hospitales de la Ciudad de México en 1992. Se obtuvo información sobre los principales factores biológicos y sociales que se asocian al bajo peso al nacer; asimismo, se determinó el estrés ocupacional utilizando el instrumento desarrollado por Karasek. Se utilizaron modelos de regresión logística para evaluar la relación de las condiciones de trabajo con el bajo peso al nacer, controlando por variables confusoras. Resultados. El bajo peso al nacer fue más alto en trabajadoras con jornadas mayores a 50 horas semanales (RM= 1.6; IC= 1.17, 2.28 y con conflictos laborales (RM= 1.5; IC= 1.0, 2.25. La falta de apoyo social tangible fue identificado como un factor de riesgo de bajo peso al nacer (RM= 1.7; IC= 1.20, 2.33. Las medidas preventivas laborales como el cambio de tareas, disminución de la jornada y las salidas por enfermedad no mostraron un efecto benéfico en el peso al nacer, a excepción de la licencia de maternidad; las madres que no la tuvieron mostraron una probabilidad 2.2 veces mayor para bajo peso al nacer (IC= 1.66, 2.93. Conclusiones. Estos hallazgos resaltan la importancia de identificar los factores de riesgo ocupacional en el embarazo..Objective. To study the association between maternal working conditions and low birth-weight in Mexico City. Material and methods. Interviews of 2 623 workers who gave birth in Mexico City hospitals during 1992 were analyzed. Information on the main biologic and social factors associated to low birth-weight was registered. Occupational stress was determined with the instrument designed by Karasek. Logistic regression models to evaluate the relationship between working conditions and low birth-weight were used, controlling by confounding variables. Results. Low birth-weight was

  6. Effect of laminaria japonica polysaccharides (LJP) on radiation damage of testis tissue in male rats

    International Nuclear Information System (INIS)

    Ren Shicheng; Luo Qiong; Yang Mingliang; Yang Jiajuan; Yan Jun; Li Zhuoneng; Wang Lihong; Cui Xiaoyan

    2007-01-01

    Objective: To observe the effect of laminaria japonica polysaccharides (LJP) on local radiation damage of testis tissue in male rats. Methods: The Wistar rats were randomly divided into 4 groups: the normal group, the model group, positive control group and LJP treatment group (50 mg·kg -1 ·d -1 ). LJP was applied to the treatment group for 10 d before local irradiation with γ-ray (6.0 Gy). The morphological change of the testis, organ index of testis and epididymides, sperm count, motility rate, superoxide dismutase (SOD) activity and malonic aldehyde (MDA) contents were measured. Results: LJP could make the damaged testis recover to near normal, elevate the organ index of testis and epididymides, promote the sperm count and motility rate, increase the activity of SOD and decrease the contents of MDA in testis tissue. Conclusions: LJP could inhibit testis tissue damage induced by local radiation, hence enhance the significant radioprotective effect to testis tissue. LJP has the conspicuous protective effect on radiation damage of testis tissue. (authors)

  7. Three synonymous genes encode calmodulin in a reptile, the Japanese tortoise, Clemmys japonica

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    Kouji Shimoda

    2002-01-01

    Full Text Available Three distinct calmodulin (CaM-encoding cDNAs were isolated from a reptile, the Japanese tortoise (Clemmys japonica, based on degenerative primer PCR. Because of synonymous codon usages, the deduced amino acid (aa sequences were exactly the same in all three genes and identical to the aa sequence of vertebrate CaM. The three cDNAs, referred to as CaM-A, -B, and -C, seemed to belong to the same type as CaMI, CaMII, and CaMIII, respectively, based on their sequence identity with those of the mammalian cDNAs and the glutamate codon biases. Northern blot analysis detected CaM-A and -B as bands corresponding to 1.8 kb, with the most abundant levels in the brain and testis, while CaM-C was detected most abundantly in the brain as bands of 1.4 and 2.0 kb. Our results indicate that, in the tortoise, CaM protein is encoded by at least three non-allelic genes, and that the ‘multigene-one protein' principle of CaM synthesis is applicable to all classes of vertebrates, from fishes to mammals.

  8. Anaerobic Digestion of Laminaria japonica Waste from Industrial Production Residues in Laboratory- and Pilot-Scale

    Science.gov (United States)

    Barbot, Yann Nicolas; Thomsen, Claudia; Thomsen, Laurenz; Benz, Roland

    2015-01-01

    The cultivation of macroalgae to supply the biofuel, pharmaceutical or food industries generates a considerable amount of organic residue, which represents a potential substrate for biomethanation. Its use optimizes the total resource exploitation by the simultaneous disposal of waste biomaterials. In this study, we explored the biochemical methane potential (BMP) and biomethane recovery of industrial Laminaria japonica waste (LJW) in batch, continuous laboratory and pilot-scale trials. Thermo-acidic pretreatment with industry-grade HCl or industrial flue gas condensate (FGC), as well as a co-digestion approach with maize silage (MS) did not improve the biomethane recovery. BMPs between 172 mL and 214 mL g−1 volatile solids (VS) were recorded. We proved the feasibility of long-term continuous anaerobic digestion with LJW as sole feedstock showing a steady biomethane production rate of 173 mL g−1 VS. The quality of fermentation residue was sufficient to serve as biofertilizer, with enriched amounts of potassium, sulfur and iron. We further demonstrated the upscaling feasibility of the process in a pilot-scale system where a CH4 recovery of 189 L kg−1 VS was achieved and a biogas composition of 55% CH4 and 38% CO2 was recorded. PMID:26393620

  9. Identification of interspecific hybrids between loquat (eriobotrya japonica lindl.) and bengal loquat (e. bengalensis hook.)

    International Nuclear Information System (INIS)

    Wang, Y.; Deng, Q.; Zeng, J.; Zhang, J.

    2017-01-01

    Loquat (Eriobotrya japonica Lindl.) is an important subtropical fruit; however, loquat fruitlets are vulnerable to cold injury in winter, which significantly decreases loquat yield in most production regions. In the present study, two loquat cultivars ('Dawuxing' and '4-1-5') and one wild loquat (E. bengalensis Hook., Bengal loquat), were used for interspecific hybridization to produce hybrids with characteristics of spring blooming to avoid cold injury of fruitlets. Hybrid seedlings were derived from direct cross (loquat as female parent and Bengal loquat as male parent) and reciprocal cross. The authenticity of 47 hybrid seedlings was confirmed using inter-simple sequence repeat (ISSR) molecular markers; and leaf morphological characteristics of the hybrid offspring and parents were preliminarily studied and compared. The results suggested that 23 true direct cross hybrids and 12 true reciprocal cross hybrids were obtained, with hybrid authenticity rates of 100 and 50.0%, respectively. Thus, a novel method of distant hybridization for loquat breeding was developed, and with their various genetic and morphological characteristics these hybrids could be valuable germplasms for horticultural use. (author)

  10. Selection of male-sterile and dwarfism genetically modified zoysia japonica through gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Bae, Tae Woong; Song, In Ja; Kang, Hong Gyu; Jeong, Ok Cheol; Sun, Hyeon Jin; Ko, Suk Min; Lim, Pyung Ok; Song, Pill Soon; Song, Sung Jun; Lee, Hyo Yeon [Jeju National University, Jeju (Korea, Republic of)

    2010-09-15

    The aim of this study is selection of the male-sterile plant for inhibiting transgene flow through gamma-irradiation ({sup 60}Co) at the pollination and fertilization cycle of herbicide-tolerant genetically modified (GM) zoysiagrass (Zoysia japonica Steud.). High frequencies of plant mutations were obtained about 18% from M{sub 1} generation at the doses (10 to 50 Gy). We also found that some M{sub 1} plants showed male-sterile plants using de-husked seeds and comparison of stainable pollen using KI-I{sub 2} solution. Besides the effects of irradiation on pollination and fertilization cycle, various other mutation like dwarf, cold tolerance, increasing grains and mass were observed. Four of dwarfism plants were selected through comparison of morphological characteristic between control and mutants during 4 years. These results demonstrated that the gamma-irradiation on pollination and fertilization cycle is very effective to induce the various mutations, and the male-sterile mutants are useful for controlling transgene flow and developing of high quality turfgasses.

  11. Selection of male-sterile and dwarfism genetically modified zoysia japonica through gamma irradiation

    International Nuclear Information System (INIS)

    Bae, Tae Woong; Song, In Ja; Kang, Hong Gyu; Jeong, Ok Cheol; Sun, Hyeon Jin; Ko, Suk Min; Lim, Pyung Ok; Song, Pill Soon; Song, Sung Jun; Lee, Hyo Yeon

    2010-01-01

    The aim of this study is selection of the male-sterile plant for inhibiting transgene flow through gamma-irradiation ( 60 Co) at the pollination and fertilization cycle of herbicide-tolerant genetically modified (GM) zoysiagrass (Zoysia japonica Steud.). High frequencies of plant mutations were obtained about 18% from M 1 generation at the doses (10 to 50 Gy). We also found that some M 1 plants showed male-sterile plants using de-husked seeds and comparison of stainable pollen using KI-I 2 solution. Besides the effects of irradiation on pollination and fertilization cycle, various other mutation like dwarf, cold tolerance, increasing grains and mass were observed. Four of dwarfism plants were selected through comparison of morphological characteristic between control and mutants during 4 years. These results demonstrated that the gamma-irradiation on pollination and fertilization cycle is very effective to induce the various mutations, and the male-sterile mutants are useful for controlling transgene flow and developing of high quality turfgasses

  12. Studies on Antiviral and Immuno-Regulation Activity of Low Molecular Weight Fucoidan from Laminaria japonica

    Science.gov (United States)

    Sun, Taohua; Zhang, Xinhui; Miao, Ying; Zhou, Yang; Shi, Jie; Yan, Meixing; Chen, Anjin

    2018-06-01

    The antiviral activity in vitro and in vivo and the effect of the immune system of two fucoidan fractions with low molecular weight and different sulfate content from Laminaria japonica (LMW fucoidans) were investigated in order to examine the possible mechanism. In vitro, I-type influenza virus, adenovirus and Parainfluenza virus I were used to infect Hep-2, Hela and MDCK cells, respectively. And 50% tissue culture infective dose was calculated to detect the antiviral activity of two LMW fucoidans. The results indicated that compared with the control group, 2 kinds of LMW fucoidans had remarkable antiviral activity in vitro in middle and high doses, while at low doses, the antiviral activity of 2 kinds of LMW fucoidans was not statistically different from that in the blank control group. And there was no statistically difference between two LMW fucoidans in antiviral activity. In vivo, LMW fucoidans could prolong the survival time of virus-infected mice, and could improve the lung index of virus-infected mice significantly, which have statistical differences with the control group significantly ( p 0.05). In this study, it was shown that both of two LMW fucoidans (LF1, LF2) could increase the thymus index, spleen index, phagocytic index, phagocytosis coefficient and half hemolysin value in middle and high doses, which suggested that LMW fucoidans could play an antiviral role by improving the quality of immune organs, improving immune cell phagocytosis and humoral immunity.

  13. Morfo-anatomia das folhas da nespereira Eriobotrya japonica Lindl., Rosaceae

    Directory of Open Access Journals (Sweden)

    W. M. de Souza

    Full Text Available Eriobotrya japonica, Rosaceae, é uma espécie conhecida popularmente como nespereira, cujas folhas são empregadas na medicina tradicional como agente hipoglicemiante e no tratamento de doenças da pele. O presente trabalho objetivou caracterizar morfo-anatomicamente as folhas, a fim de fornecer subsídios farmacognósticos ao controle de qualidade da droga vegetal e taxonômicos à família. O material botânico foi fixado e submetido às técnicas usuais de microscopia ótica e eletrônica de varredura. As folhas são simples, alternas, lanceoladas, de limbo inteiro e margem denteada. A epiderme foliar é uniestratificada e revestida por cutícula espessada e levemente estriada. Tricomas tectores unicelulares longos são encontrados em ambas as faces, sendo numerosos na abaxial. A folha é hipoestomática, revelando estômatos anomocíticos. O mesofilo é dorsiventral e os feixes vasculares de pequeno porte são colaterais e podem apresentar extensão de bainha. A nervura principal possui formato biconvexo e o pecíolo circular, sendo ambos percorridos por feixes vasculares anficrivais. Foram observados idioblastos contendo cristais de oxalato de cálcio, mucilagem e compostos fenólicos.

  14. Antioxidant Activities of Achyranthes japonica Nakai Extract and Its Application to the Pork Sausages

    Science.gov (United States)

    Park, J. H.; Kang, S. N.; Shin, D.; Hur, I. C.; Kim, I. S.; Jin, S. K.

    2013-01-01

    Influence of Achyranthes japonica Nakai Extract (AJNE) on properties of pork sausages were studied in the present investigation. AJNE was added to sausages alone or in combination with ascorbic acid to obtain a comparative analysis on properties of control and ascorbic acid added-sausages. Results showed that addition of 0.05% AJNE led to a decrease in color L* and whiteness (W), and an increase in color b* of pork sausage samples (psausages containing AJNE was not significantly different, ascorbic acid added-sausages were highest amongst other treatments (pSausages containing AJNE had lower non-heme iron values and peroxide value (POV) than control sausages (psausages (psausages (psausages. Free radical scavenging analysis showed that AJNE did not affect 1,1-diphenyl -2-picrylhydrazyl (DPPH) activity of sausages, whereas ascorbic acid added-sausages showed relatively higher activity among the samples (psausages. In sensory evaluation, AJNE treatment had significant effects on color (psausages, and appears to be particularly effective in inducing changes in non-heme iron concentration, POV value and nitrosomyglobin content. PMID:25049789

  15. Degradation of sulfated polysaccharide extracted from algal Laminaria japonica and its modulation on calcium oxalate crystallization

    Energy Technology Data Exchange (ETDEWEB)

    Ouyang Jianming, E-mail: toyjm@jnu.edu.cn [Department of Chemistry, Jinan University, Guangzhou 510632 (China); Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632 (China); Wang Miao; Lu Peng; Tan Jin [Department of Chemistry, Jinan University, Guangzhou 510632 (China); Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632 (China)

    2010-08-30

    Sulfated polysaccharide (LPS) extracted from alga Laminaria japonica was degraded by hydrogen peroxide (H{sub 2}O{sub 2}). The average molecular weight of LPS was apparently decreased from 172,000 to 9550 after degradation, while the proportion of sulfate groups (-OSO{sub 3}{sup -}) and carboxylic groups (-COO{sup -}) in the molecular chains of LPS were slightly decreased from 4.5% and 5.20% to 3.9% and 4.64%, respectively. The effects of degraded and natural LPS on formation of calcium oxalate (CaOxa) crystals were investigated in vitro using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), zeta-potential, and atomic absorption spectroscopy. LPS could increase the concentration of soluble Ca{sup 2+} ions in the solution, decrease the weight of precipitated CaOxa, and increase the negative value of zeta-potential of CaOxa crystals. LPS also inhibits the formation of thermodynamically stable calcium oxalate monohydrate (COM) crystals, while inducing and stabilizing metastable calcium oxalate dihydrate (COD) crystals. These results suggested that both degraded and natural LPS could decrease CaOxa crystallization, but the inhibition efficiency of the degraded LPS was clearly superior to that of the natural LPS. We expected this investigation would provide encouragement for further exploration into new drugs for the prevention and treatment of urolithiasis.

  16. Influence of cooking on anthocyanins in black rice (Oryza sativa L. japonica var. SBR).

    Science.gov (United States)

    Hiemori, Miki; Koh, Eunmi; Mitchell, Alyson E

    2009-03-11

    The composition and thermal stability of anthocyanins in black rice (Oryza sativa L. japonica var. SBR) produced in California were investigated. Six anthocyanin pigments were identified and quantified by high performance liquid chromatography using photo diode-array detection (HPLC-PDA) and electrospray ionization mass spectrometry [LC-(ESI)MS/MS]. The predominant anthocyanins are cyanidin-3-glucoside (572.47 microg/g; 91.13% of total) and peonidin-3-glucoside (29.78 microg/g; 4.74% of total). Minor constituents included three cyanidin-dihexoside isomers and one cyanidin hexoside. Thermal stability of anthocyanins was assessed in rice cooked using a rice cooker, pressure cooker, or on a gas range. All cooking methods caused significant (P rice cooker (74.2%) and gas range (65.4%). Conversely, levels of protocatechuic acid increased 2.7 to 3.4 times in response to all cooking methods. These findings indicate that cooking black rice results in the thermal degradation of cyanidin-3-glucoside and concomitant production of protocatechuic acid.

  17. Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana

    Science.gov (United States)

    Itoh, Takeshi; Tanaka, Tsuyoshi; Barrero, Roberto A.; Yamasaki, Chisato; Fujii, Yasuyuki; Hilton, Phillip B.; Antonio, Baltazar A.; Aono, Hideo; Apweiler, Rolf; Bruskiewich, Richard; Bureau, Thomas; Burr, Frances; Costa de Oliveira, Antonio; Fuks, Galina; Habara, Takuya; Haberer, Georg; Han, Bin; Harada, Erimi; Hiraki, Aiko T.; Hirochika, Hirohiko; Hoen, Douglas; Hokari, Hiroki; Hosokawa, Satomi; Hsing, Yue; Ikawa, Hiroshi; Ikeo, Kazuho; Imanishi, Tadashi; Ito, Yukiyo; Jaiswal, Pankaj; Kanno, Masako; Kawahara, Yoshihiro; Kawamura, Toshiyuki; Kawashima, Hiroaki; Khurana, Jitendra P.; Kikuchi, Shoshi; Komatsu, Setsuko; Koyanagi, Kanako O.; Kubooka, Hiromi; Lieberherr, Damien; Lin, Yao-Cheng; Lonsdale, David; Matsumoto, Takashi; Matsuya, Akihiro; McCombie, W. Richard; Messing, Joachim; Miyao, Akio; Mulder, Nicola; Nagamura, Yoshiaki; Nam, Jongmin; Namiki, Nobukazu; Numa, Hisataka; Nurimoto, Shin; O’Donovan, Claire; Ohyanagi, Hajime; Okido, Toshihisa; OOta, Satoshi; Osato, Naoki; Palmer, Lance E.; Quetier, Francis; Raghuvanshi, Saurabh; Saichi, Naomi; Sakai, Hiroaki; Sakai, Yasumichi; Sakata, Katsumi; Sakurai, Tetsuya; Sato, Fumihiko; Sato, Yoshiharu; Schoof, Heiko; Seki, Motoaki; Shibata, Michie; Shimizu, Yuji; Shinozaki, Kazuo; Shinso, Yuji; Singh, Nagendra K.; Smith-White, Brian; Takeda, Jun-ichi; Tanino, Motohiko; Tatusova, Tatiana; Thongjuea, Supat; Todokoro, Fusano; Tsugane, Mika; Tyagi, Akhilesh K.; Vanavichit, Apichart; Wang, Aihui; Wing, Rod A.; Yamaguchi, Kaori; Yamamoto, Mayu; Yamamoto, Naoyuki; Yu, Yeisoo; Zhang, Hao; Zhao, Qiang; Higo, Kenichi; Burr, Benjamin; Gojobori, Takashi; Sasaki, Takuji

    2007-01-01

    We present here the annotation of the complete genome of rice Oryza sativa L. ssp. japonica cultivar Nipponbare. All functional annotations for proteins and non-protein-coding RNA (npRNA) candidates were manually curated. Functions were identified or inferred in 19,969 (70%) of the proteins, and 131 possible npRNAs (including 58 antisense transcripts) were found. Almost 5000 annotated protein-coding genes were found to be disrupted in insertional mutant lines, which will accelerate future experimental validation of the annotations. The rice loci were determined by using cDNA sequences obtained from rice and other representative cereals. Our conservative estimate based on these loci and an extrapolation suggested that the gene number of rice is ∼32,000, which is smaller than previous estimates. We conducted comparative analyses between rice and Arabidopsis thaliana and found that both genomes possessed several lineage-specific genes, which might account for the observed differences between these species, while they had similar sets of predicted functional domains among the protein sequences. A system to control translational efficiency seems to be conserved across large evolutionary distances. Moreover, the evolutionary process of protein-coding genes was examined. Our results suggest that natural selection may have played a role for duplicated genes in both species, so that duplication was suppressed or favored in a manner that depended on the function of a gene. PMID:17210932

  18. Crystallization and preliminary X-ray crystallographic analysis of dioscorin from Dioscorea japonica

    International Nuclear Information System (INIS)

    Xue, You-Lin; Miyakawa, Takuya; Sawano, Yoriko; Tanokura, Masaru

    2012-01-01

    Dioscorin from D. japonica was expressed, purified and crystallized using the sitting-drop vapour-diffusion method. The dioscorin crystal diffracted X-rays to 2.11 Å resolution. Dioscorin, the major tuber storage protein in yam, has been reported to possess carbonic anhydrase, trypsin inhibitor, angiotensin-converting enzyme (ACE) inhibitor, free-radical scavenger, dehydroascorbate reductase and monodehydroascorbate reductase activities. Recent research has also found that dioscorin can enhance immune modulation via the toll-like receptor 4 (TLR-4) signal transduction pathway in RAW 264.7 cells, murine bone-marrow cells and human monocytes ex vivo. Resolving the structure of dioscorin would help in better understanding its activities and would provide clues to understanding the mechanism of its multiple functions. The full-length protein (residues 1–246) with an additional His 6 tag at the N-terminus was expressed in Escherichia coli Rosetta (DE3) cells. After His-tag cleavage and purification, the protein was crystallized by the sitting-drop vapour-diffusion method at 278 K. An X-ray diffraction data set was collected to a resolution of 2.11 Å using a synchrotron X-ray source. The crystal belonged to space group C222 1 , with unit-cell parameters a = 83.5, b = 156.8, c = 83.6 Å, and was estimated to contain two protein molecules per asymmetric unit

  19. The role of silicon in physiology of the medicinal plant (Lonicera japonica L.) under salt stress

    Science.gov (United States)

    Gengmao, Zhao; Shihui, Li; Xing, Sun; Yizhou, Wang; Zipan, Chang

    2015-08-01

    Silicon(Si) is the only element which can enhance the resistance to multiple stresses. However, the role of silicon in medicinal plants under salt stress is not yet understood. This experiment was conducted to study the effects of silicon addition on the growth, osmotic adjustments, photosynthetic characteristics, chloroplast ultrastructure and Chlorogenic acid (CGA) production of Honeysuckle plant (Lonicera japonica L.) under salt-stressed conditions. Salinity exerted an adverse effect on the plant fresh weight and dry weight, whilst 0.5 g L-1 K2SiO3·nH2O addition obviously improved the plant growth. Although Na+ concentration in plant organs was drastically increased with increasing salinity, higher levels of K+/Na+ ratio was obtained after K2SiO3·nH2O addition. Salinity stress induced the destruction of the chloroplast envelope; however, K2SiO3·nH2O addition counteracted the adverse effect by salinity on the structure of the photosynthetic apparatus. K2SiO3·nH2O addition also enhanced the activities of superoxide dismutase and catalase. To sum up, exogenous Si plays a key role in enhancing its resistance to salt stresses in physiological base, thereby improving the growth and CGA production of Honeysuckle plant.

  20. New flavonol glycosides from the leaves of Triantha japonica and Tofieldia nuda.

    Science.gov (United States)

    Iwashina, Tsukasa; Tamura, Minoru N; Murai, Yoshinori; Kitajima, Junichi

    2013-09-01

    Two new flavonol glycosides were isolated from the leaves of Triantha japonica, together with eight known flavonols, kaempferol 3-O-sophoroside, kaempferol 3-O-sambubioside, kaempferol 3-O-glucosyl-(1 --> 2)-[glucosyl-(1 --> 6)-glucoside], quercetin 3-O-sophoroside, quercetin 3-O-sambubioside, isorhamnetin 3-O-glucoside, isorhamnetin 3-O-sophoroside and isorhamnetin 3-O-sambubioside. The new compounds were identified as kaempferol 3-O-beta-xylopyranosyl-(1 --> 2)-[beta-glucopyranosyl-(1 --> 6)-beta-glucopyranoside] (1) and isorhamnetin 3-O-beta-xylopyranosyl-(1 --> 2)-[beta-glucopyranosyl-(1 --> 6)-beta-glucopyranoside] (3) by UV, LC-MS, acid hydrolysis, and 1H and 13C NMR spectroscopy. Another two new flavonol glycosides were isolated from theleaves of Tofieldia nuda, and identified as kaempferol 3-O-beta-glucopyranosyl-(1 --> 2)-[beta-glucopyranosyl-(1 --> 6)-beta-galactopyranoside] (4) and quercetin 3-O-beta-glucopyranosyl-(1 --> 2)-[beta-glucopyranosyl-(1 --> 6)-beta-galactopyranoside] (5). Though the genera Triantha and Tofieldia were treated as Tofieldia sensu lato, they were recently divided into two genera. It was shown by this survey that their flavonoid composition were also different to each other.

  1. Anaerobic Digestion of Laminaria japonica Waste from Industrial Production Residues in Laboratory- and Pilot-Scale

    Directory of Open Access Journals (Sweden)

    Yann Nicolas Barbot

    2015-09-01

    Full Text Available The cultivation of macroalgae to supply the biofuel, pharmaceutical or food industries generates a considerable amount of organic residue, which represents a potential substrate for biomethanation. Its use optimizes the total resource exploitation by the simultaneous disposal of waste biomaterials. In this study, we explored the biochemical methane potential (BMP and biomethane recovery of industrial Laminaria japonica waste (LJW in batch, continuous laboratory and pilot-scale trials. Thermo-acidic pretreatment with industry-grade HCl or industrial flue gas condensate (FGC, as well as a co-digestion approach with maize silage (MS did not improve the biomethane recovery. BMPs between 172 mL and 214 mL g−1 volatile solids (VS were recorded. We proved the feasibility of long-term continuous anaerobic digestion with LJW as sole feedstock showing a steady biomethane production rate of 173 mL g−1 VS. The quality of fermentation residue was sufficient to serve as biofertilizer, with enriched amounts of potassium, sulfur and iron. We further demonstrated the upscaling feasibility of the process in a pilot-scale system where a CH4 recovery of 189 L kg−1 VS was achieved and a biogas composition of 55% CH4 and 38% CO2 was recorded.

  2. Anaerobic Digestion of Laminaria japonica Waste from Industrial Production Residues in Laboratory- and Pilot-Scale.

    Science.gov (United States)

    Barbot, Yann Nicolas; Thomsen, Claudia; Thomsen, Laurenz; Benz, Roland

    2015-09-18

    The cultivation of macroalgae to supply the biofuel, pharmaceutical or food industries generates a considerable amount of organic residue, which represents a potential substrate for biomethanation. Its use optimizes the total resource exploitation by the simultaneous disposal of waste biomaterials. In this study, we explored the biochemical methane potential (BMP) and biomethane recovery of industrial Laminaria japonica waste (LJW) in batch, continuous laboratory and pilot-scale trials. Thermo-acidic pretreatment with industry-grade HCl or industrial flue gas condensate (FGC), as well as a co-digestion approach with maize silage (MS) did not improve the biomethane recovery. BMPs between 172 mL and 214 mL g(-1) volatile solids (VS) were recorded. We proved the feasibility of long-term continuous anaerobic digestion with LJW as sole feedstock showing a steady biomethane production rate of 173 mL g(-1) VS. The quality of fermentation residue was sufficient to serve as biofertilizer, with enriched amounts of potassium, sulfur and iron. We further demonstrated the upscaling feasibility of the process in a pilot-scale system where a CH₄ recovery of 189 L kg(-1) VS was achieved and a biogas composition of 55% CH₄ and 38% CO₂ was recorded.

  3. Management of Meloidogyne incognita on tomato with endophytic bacteria and fresh residue of Wasabia japonica.

    Science.gov (United States)

    Li, G J; Dong, Q E; Ma, L; Huang, Y; Zhu, M L; Ji, Y P; Wang, Q H; Mo, M H; Zhang, K Q

    2014-10-01

    To characterize the nematicidal endophytic bacteria (NEB) of Wasabia japonica (wasabi) and evaluated the control efficacies of promising NEB as well as fresh wasabi residue (FWR) against Meloidogyne incognita on tomato. By in vitro bioassay, 53 NEB strains showing nematicidal efficacies of >50% against J2 of M. incognita were isolated from wasabi. Basing on 16S rRNA gene sequences, these NEB were identified into 18 species of 11 genera. In greenhouse, incorporation of selected NEB culture or FWR into potted soil significantly reduced infection of M. incognita on tomato. Treating tomatoes with either FWR or NEB of Raoultella terrigena RN16 and Pseudomonas reinekei SN21 in the field yielded excellent control efficacies against M. incognita, especially the combinations of FWR with either R. terrigena RN16 or Ps. reinekei SN21 at doses of 50 g plus 100 ml per plant or more. The results established that R. terrigena RN16 and Ps. reinekei SN21 applied separately or combined with FWR have the potential to provide bioprotection agents against M. incognita. This study provides novel way for disease management using combination of endophyte and host residue. © 2014 The Society for Applied Microbiology.

  4. Distribution of Peripheral Memory T Follicular Helper Cells in Patients with Schistosomiasis Japonica.

    Directory of Open Access Journals (Sweden)

    Xiaojun Chen

    Full Text Available Schistosomiasis is a helminthic disease that affects more than 200 million people. An effective vaccine would be a major step towards eliminating the disease. Studies suggest that T follicular helper (Tfh cells provide help to B cells to generate the long-term humoral immunity, which would be a crucial component of successful vaccines. Thus, understanding the biological characteristics of Tfh cells in patients with schistosomiasis, which has never been explored, is essential for vaccine design.In this study, we investigated the biological characteristics of peripheral memory Tfh cells in schistosomiasis patients by flow cytometry. Our data showed that the frequencies of total and activated peripheral memory Tfh cells in patients were significantly increased during Schistosoma japonicum infection. Moreover, Tfh2 cells, which were reported to be a specific subpopulation to facilitate the generation of protective antibodies, were increased more greatly than other subpopulations of total peripheral memory Tfh cells in patients with schistosomiasis japonica. More importantly, our result showed significant correlations of the percentage of Tfh2 cells with both the frequency of plasma cells and the level of IgG antibody. In addition, our results showed that the percentage of T follicular regulatory (Tfr cells was also increased in patients with schistosomiasis.Our report is the first characterization of peripheral memory Tfh cells in schistosomasis patients, which not only provides potential targets to improve immune response to vaccination, but also is important for the development of vaccination strategies to control schistosomiasis.

  5. Biological Activities of Extracts from Loquat (Eriobotrya japonica Lindl.: A Review

    Directory of Open Access Journals (Sweden)

    Yilong Liu

    2016-12-01

    Full Text Available Loquat (Eriobotrya japonica Lindl. is a subtropical fruit tree with high medicinal value native to China. Different organs of loquat have been used historically as folk medicines and this has been recorded in Chinese history for thousands of years. Research shows that loquat extracts contain many antioxidants, and different extracts exhibit bioactivity capable of counteracting inflammation, diabetes, cancer, bacterial infection, aging, pain, allergy and other health issues. Bioactive compounds such as phenolics and terpenoids have been isolated and characterized to provide a better understanding of the chemical mechanisms underlying the biological activities of loquat extracts. As the identification of compounds progresses, studies investigating the in vivo metabolism, bioavailability, and structure–activity relationships, as well as potential toxicity of loquat extracts in animal or cell models are receiving more attention. In addition, genetic studies and breeding of loquat germplasms for high contents of health-benefiting compounds may provide new insight for the loquat industry and research. This review is focused on the main medicinal properties reported and the possible pharmaceutically active compounds identified in different loquat extracts.

  6. Usos del celular en jóvenes chilenos de bajos recursos

    OpenAIRE

    Margarita Pedreros-Gajardo; Luis Araya-Castillo

    2013-01-01

    En este trabajo analizamos los significados que los jóvenes y las jóvenes de Chile de bajos recursos atribuyen a la compra, uso y tenencia de un celular. La investigación cualitativa utilizada no sólo nos permitió descubrir las necesidades que los sujetos jóvenes buscan satisfacer con la compra y/o uso de un celular, sino también los factores socioculturales que determinan este comportamiento de compra. La importancia del presente no es sólo por la pretensión de ser un estudio sociológico sob...

  7. Bluetooth 4.0 Low Energy: la futura solucion inalambrica de bajo consumo

    OpenAIRE

    Morales Pedro, Ricard

    2011-01-01

    En los últimos años el sector de las telecomunicaciones se ha erigido como uno de los mas innovadores de nuestra sociedad, donde la irrupción de nuevos estandares de comunicación inalambrica esta permitiendo ir eliminando la necesidad de los cables de transmisión para conectar dispositivos. Con este proposito nació Bluetooth, una tecnologia que permite transmisiones inalambricas de corto alcance de forma robusta y sencilla, junto con un consumo reducido y un bajo coste. Tras la aparición de B...

  8. Sobre un modelo de Holthausen para la empresa competitiva bajo incertidumbre en el precio

    OpenAIRE

    Álvarez López, A. A.

    1999-01-01

    El modelo presentado por HOLTHAUSEN (1979) amplía el modelo original de SANDMO (1971) para una empresa competitiva bajo incertidumbre en el precio y aversión al riesgo. La aportación esencial de HOLTHAUSEN es la de considerar que existe un mercado de futuros para el bien que la empresa produce, lo que causa que el comportamiento de ésta frente al riesgo sea diferente. En este trabajo planteamos el problema de decisión de la empresa del modelo de HOLTHAUSEN como un problema de optimización de ...

  9. Efectos del bajo peso materno preconcepcional sobre el embarazo y el parto

    OpenAIRE

    López Montero, Maribel; Trufero Cánovas, Norma

    2006-01-01

    Se realizó un estudio de cohorte no concurrente pareado 1:1 para investigar los efectos del bajo peso materno preconcepcional (malnutrición materna por defecto) sobre el embarazo, el parto y el recién nacido en el Policlínico Finlay del municipio Camagüey desde octubre de 2002 hasta abril de 2004. Fueron estudiadas 51 gestantes malnutridas por defecto y 51 embarazadas sin riesgo. Los datos fueron tomados a partir de los registros oficiales de la atención perinatal complementados con una encue...

  10. Alumnos con Bajo Rendimiento Escolar, los blogs y el tutor de Primaria

    OpenAIRE

    Rico-Sánchez, Jesús David

    2013-01-01

    Teniendo en cuenta el problema que tiene el sistema educativo español con un índice muy elevado de fracaso escolar, se pretendió investigar concretamente sobre los alumnos del sexto curso de Primaria que presentan Bajo Rendimiento Escolar aprovechando los avances tecnológicos existentes en la actualidad y que han penetrado con gran fuerza en la sociedad. Por ello, el problema planteado en este trabajo es estudiar si el potencial motivador, comunicativo y utilitario del Edublog puede ayudar a ...

  11. La identificación de espacios mineros e industriales abandonados: el caso del Bajo Besaya

    OpenAIRE

    García Valiente, Nieves; Nogués Linares, Soledad

    2014-01-01

    La recuperación de los terrenos conocidos como “brownfields” es un tema recurrente en las políticas del suelo y el medio ambiente de la Unión Europea para el horizonte 2020. Con una tradición eminentemente industrial y minera, el Bajo Besaya presenta, en la actualidad, amplios espacios abandonados degradados, que son el resultado del cese de las actividades productivas anteriores. Éstos suponen un detrimento para la zona, por lo que es necesaria su transformación de pasivos ambientales y soci...

  12. Análisis del bajo peso en la atención primaria

    Directory of Open Access Journals (Sweden)

    Miguel Lugones Botell

    1995-08-01

    Full Text Available Se realiza un estudio retrospectivo de algunos aspectos relacionados con la atención prenatal de las gestantes y su análisis correspondiente, que tuvieron recién nacidos de bajo peso en el período comprendido entre 1988 y 1991, ambos inclusive. Se concluye que los principales factores relacionados con éste fueron: nuliparidad, madre trabajadora, poca ganancia de peso en el embarazo y entre las afecciones asociadas con la gestación encontramos la amenaza de aborto, la anemia y la enfermedad hipertensiva del embarazo, entre las más frecuentes.

  13. Análisis del amortiguamiento en vidrio estructural bajo cargas de impacto

    OpenAIRE

    Ramos, Alberto; Pelayo, F.; Lamela, María Jesús; Fernández Canteli, Alfonso; Huerta Gómez de Merodio, María Consuelo; Pacios Alvarez, Antonia

    2013-01-01

    Los últimos avances tecnológicos y las tendencias arquitectónicas actuales han impulsado notablemente el uso de una gran variedad de productos de vidrio en la construcción que, en función de sus propias características, permiten diseñar y calcular elementos estructurales de este material bajo condiciones de seguridad. Este trabajo presenta la evaluación y el análisis de las propiedades de amortiguamiento de placas rectangulares de vidrio laminado de 1938 mm x 876 mm con distinto espesor, segú...

  14. Corrosión microbiológica en aceros de bajo carbono

    OpenAIRE

    Medina-Custodio, O; Ortiz-Prado, A; Jacobo-Armendáriz, V.H.; Schouwenaars-Franssens, R

    2009-01-01

    La corrosión microbiológica es un tipo común de deterioro que afecta diversas industrias, una de ellas es la petrolera en la que se estiman que el 20% o 30% de fallas en las tuberías de trasporte de hidrocarburos es favorecida por microorganismos. Las reacciones químicas que sustentan éstos, generan transferencia de iones, lo que justifica el empleo de técnicas electroquímicas para su análisis. En este trabajo, se estudiaron probetas de acero de bajo carbono SAE 1018, sumergidas en un medio n...

  15. Involucramiento parental escolar en alumnos de bachillerato de alto y bajo rendimiento

    OpenAIRE

    Ortega Pierres, Susana; Andrade, Patricia; Vásquez Olmedo, Claudia Elena

    2016-01-01

    El Sistema Educativo Mexicano enfrenta problemas como bajo desempeño y reprobación, circunstancias que anteceden el abandono escolar, el cual se ubica como un fenómeno multicausal, en el que además de los diversos factores relacionados (personales, familiares, escolares, económicos sociales, etc.) se caracteriza por una tendencia a incrementarse conforme los alumnos avanzan en la escuela. De 100 alumnos que ingresan a primaria, 24 concluyen el nivel medio superior y de estos solo 14 concluyen...

  16. Evaluación de un cluster bajo agricultura protegida en México

    OpenAIRE

    Padilla-Bernal, Luz Evelia; Reyes-Rivas, Eliver; Pérez-Veyna, Óscar

    2012-01-01

    El naciente cluster de tomate bajo agricultura protegida en Zacatecas enfrenta el reto de participar en los mercados globales de una manera sostenible; una respuesta viable para ello es elevar su capacidad de upgrading. En este trabajo se determina la relación entre la eficiencia colectiva en el upgrading del cluster. La información se obtuvo a través de un cuestionario aplicado a técnicos de las unidades de producción (UP), el cual se complementó con entrevistas a propietarios o gerentes, in...

  17. La importancia de los materiales en las viviendas de muy bajo coste

    OpenAIRE

    Salas, Julián

    1992-01-01

    El texto que sigue se encuadra en el contexto de intentar enmarcar la importancia considerable del capítulo de los materiales de construcción en el subsector de las viviendas de muy bajo coste en los Países en Vías de Desarrollo en general, y en Latinoamérica en particular. El autor trata de cuantificar, en forma global, la repercusión de los materiales en varios supuestos y como ésta aumenta al disminuir —prácticamente desaparecer— las partidas de instalaciones y acabados. De conformidad...

  18. Next-Generation Sequencing of an 88-Year-Old Specimen of the Poorly Known Species Liagora japonica (Nemaliales, Rhodophyta) Supports the Recognition of Otohimella gen. nov.

    Science.gov (United States)

    Suzuki, Masahiro; Segawa, Takahiro; Mori, Hiroshi; Akiyoshi, Ayumi; Ootsuki, Ryo; Kurihara, Akira; Sakayama, Hidetoshi; Kitayama, Taiju; Abe, Tsuyoshi; Kogame, Kazuhiro; Kawai, Hiroshi; Nozaki, Hisayoshi

    2016-01-01

    Liagora japonica is a red algal species distributed in temperate regions of Japan. This species has not been collected from its type locality on the Pacific coast of Japan since 1927 and seems to have become extinct in this area. For molecular characterization of L. japonica, we extracted DNA from the topotype material of L. japonica collected in 1927, analyzed seven genes using Illumina next-generation sequencing, and compared these data with sequences from modern samples of similar red algae collected from the Japan Sea coast of Japan. Both morphological and molecular data from modern samples and historical specimens (including the lectotype and topotype) suggest that the specimens from the Pacific and Japan Sea coasts of Japan should be treated as a single species, and that L. japonica is phylogenetically separated from the genus Liagora. Based on the phylogenetic results and examination of reproductive structures, we propose Otohimella japonica gen. et comb. nov., characterized morphologically by diffuse carposporophytes, undivided carposporangia, and involucral filaments initiated only from the cortical cell on the supporting cell.

  19. Restos de vida en Bajo este sol tremendo de Carlos Busqued

    Directory of Open Access Journals (Sweden)

    Marcos Seifert

    2017-07-01

    Full Text Available Este trabajo se propone analizar cómo la novela de Carlos Busqued Bajo este sol tremendo (2009 se apoya en la representación de vidas desechadas, basura y todo tipo de restos para instalarse en una tensión entre orden y exceso. La narración plantea una lógica en la que la basura, en lugar de constituirse en un indicio que posibilite la reconstrucción del paisaje social, exhibe lo desbordante, lo inasimilable y los vacíos de sentido. En el mundo que nos presenta Busqued lo viviente se vuelve resto en la medida en que encarna una heterogeneidad irreductible a cualquier estructura social y productiva. This paper analyzes the way in which Carlos Busqued's novel Bajo este sol tremendo (2009 is based on the representation of discarded lives, garbage, and all kinds of remains and thus proposes a tension between order and excess. The narration raises a logic in which garbage, instead of constituting an indicator that allows for the reconstruction of the social landscape, exhibits the overflowing, the unassimilable, and the voids of meaning. In the world that Busqued presents, the living becomes a remainder because it embodies an irreducible heterogeneity with regards to any social and productive structure.

  20. Materiales, tecnologías y prototipos de viviendas de muy bajo coste

    Directory of Open Access Journals (Sweden)

    Salas Serrano, Julián

    1982-11-01

    Full Text Available Not available.El fin último de la investigación es el de conseguir unas tipologías de viviendas realizadas mediante materiales, procedimientos o sistemas constructivos que permitan proporcionar un producto final, cuyas características fundamentales sean: adecuación a las necesidades físicas y socioculturales de la zona y, prioritariamente, la consecución de un muy bajo coste da ejecución de las mismas. Cuando se habla a continuación de «viviendas de muy bajo coste» se debe entender que nos referimos a las que cumplen básicamente las dos condiciones limitativas aceptadas por las Naciones Unidas (UNCHSHABITAT: a Superficie construida comprendida entre 30 y 50 m2. b Coste de construcción inferior a los 100 $ USA (1982 por metro cuadrado construido. (Para nuestras condiciones, fijaríamos un coste aproximado de 10.000 pts/m2.

  1. Algunas variables bioquímicas en adolescentes obesos con antecedente de bajo peso al nacer

    Directory of Open Access Journals (Sweden)

    María del Carmen Valdés Alonso

    Full Text Available Introducción: la malnutrición intraútero ha sido postulada con vinculación a afecciones del desarrollo embrionario que determinan el desarrollo ulterior de obesidad, diabetes mellitus tipo 2, dislipidemias, disfunción mitocondrial y aterosclerosis. Objetivo: determinar las complicaciones metabólicas en adolescentes obesos con antecedentes de bajo peso al nacer. Métodos: estudio descriptivo y de corte transversal. Se incluyeron 50 pacientes obesos, con antecedentes de bajo peso al nacer y embarazo a término, de ambos sexos, en las edades comprendidas entre 9 y 17 años de edad, que asistieron a la consulta externa del servicio de Endocrinología del Hospital Pediátrico Docente "Juan Manuel Márquez", en el período comprendido de enero de 2011 a enero de 2012. Se realizaron determinaciones de colesterol, triglicéridos, aminotransferasas hepáticas, glucemia, e insulinemia en ayunas y posprandial de dos horas. Resultados: las variables bioquímicas estudiadas no mostraron diferencias entre los puntos de corte utilizados para clasificar el peso al nacer. La transaminasa glutámico pirúvica mostró valores más altos en los adolescentes entre 15 y 17 años de edad. Conclusiones: la hipertrigliceridemia fue más elevada en el sexo femenino, mientras que la hiperglucemia y la hiperinsulinemia lo fueron en el masculino.

  2. Glosa paseada bajo el fuego y la lluvia: cinco lentes para mirar el Chocó

    Directory of Open Access Journals (Sweden)

    Mónica María del Valle Idárraga

    2011-12-01

    Full Text Available A partir de la perspectiva general de la ecocrítica o ecopoética, este artículo analiza los modos como se representa la naturaleza y su relación con lo humano en la obra Glosa paseada bajo el fuego y la lluvia (1982 del escritor chocoano Carlos Arturo Caicedo Licona. Las cinco instancias de esa figuración que aquí se estudian ayudan a crear un universo simbólico que respalda una idea de chocoanidad cifrada en prácticas, creencias, recorridos y visiones desde un marco anterior a tecnologías de la identidad como la biodiversidad o la patrimonialización.Based on the general premises of Ecocriticism, this article explores the ways in which nature and its inter-relation to human beings is created in Glosa paseada bajo el fuego y la lluvia, a work by Carlos Arturo Caicedo Licona, a writer from Chocó, Colombia. Five instances of that representation are analyzed as pieces that, while describing practices, conceptions, beliefs and cartographic descriptions, structure an idea of Chocoan identity previous to current identity technologies, such as patrimonialization and biodiversity.

  3. THE CONSTRUCTION AND CONTESTATION OF ISLAMIC IDENTITY OF THE BAJO PEOPLE IN WAKATOBI ISLANDS, SOUTH EAST SULAWESI, INDONESIA

    Directory of Open Access Journals (Sweden)

    Benny Baskara

    2014-02-01

    Full Text Available On the one one hand, the Bajo people are well-known as ‘the sea people’ who have their own indigenous belief system – belief in the Lord of the Sea. On the other hand, they also acknowledge themselves as Muslims. Thus, the religious identity of the Bajo people reflects a unique combination of Islamic teachings and their indigenous beliefs. This unique combination is not only expressed in their religious life, in their rituals and worship practices, but also in the values found in their customary practices (adat as a guiding system for their life. This paper examines the construction and contestation of the religious identity of the Bajo people, especially how they construct their identities in relation to their natural, social, and religious environments. The contestation of the Bajo religious identity covers three aspects: the contestation of the Islam of ‘the sea people’ against the Islam of ‘the land people’, the contenstation of the ‘official’ Islam against the ‘traditional’ Islam, and the challenges of modernity, especially the commodification of the Bajo religious expressions. This contestation, in turn, reconstructs their religious identity into a more adaptive one.

  4. Use of gamma-rays sensitivity for discrimination of upland cultivars of groups Indican and Japonica; Uso da sensitividade a radiacao gama na discriminacao de variedades de arroz-de-sequeiro dos grupos Indica e Japonica

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Luis Roberto Franco [UNESP, Botucatu, SP (Brazil). Dept. de Genetica; Ando, Akihiko [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil)

    2003-07-01

    Sixty-five upland rice cultivars (Oryza sativa L.) were evaluated in relation to gamma-ray sensitivity. Seeds were subjected to seven doses of gamma-radiation and sown in wooden boxes in randomised complete block design with three replications. The experiment was conducted in greenhouse during the year of 1992. Physiological effects caused by radiation in the M{sub 1} generation, were evaluated. The results showed that the sensitivity to the radiation at doses 300 and 360 Gy was useful for distinguishing Indican and Japonica groups. (author)

  5. Ability of Rf5 and Rf6 to Restore Fertility of Chinsurah Boro II-type Cytoplasmic Male Sterile Oryza Sativa (ssp. Japonica) Lines.

    Science.gov (United States)

    Zhang, Honggen; Che, Jianlan; Ge, Yongshen; Pei, Yan; Zhang, Lijia; Liu, Qiaoquan; Gu, Minghong; Tang, Shuzhu

    2017-12-01

    Three-line Oryza sativa (ssp. japonica) hybrids have been developed mainly using Chinsurah Boro II (BT)-type cytoplasmic male sterility (CMS). The Rf1 gene restores the fertility of BT-type CMS lines, and is the only fertility restorer gene (Rf) that has been used to produce three-line japonica hybrids. Using more Rf genes to breed BT-type restorer lines may broaden the genetic diversity of the restorer lines, and represents a viable approach to improve the heterosis level of BT-type japonica hybrids. We identified two major Rf genes from '93-11' that are involved in restoring the fertility of BT-type CMS plants. These genes were identified from resequenced chromosome segment substitution lines derived from a cross between the japonica variety 'Nipponbare' and the indica variety '93-11'. Molecular mapping results revealed that these genes were Rf5 and Rf6, which are the Rf genes that restore fertility to Honglian-type CMS lines. The BT-type F 1 hybrids with either Rf5 or Rf6 exhibited normal seed setting rates, but F 1 plants carrying Rf6 showed more stable seed setting rates than those of plants carrying Rf5 under heat-stress conditions. Furthermore, the seed setting rates of F 1 hybrids carrying both Rf5 and Rf6 were more stable than that of F 1 plants carrying only one Rf gene. Rf6 is an important genetic resource for the breeding of BT-type japonica restorer lines. Our findings may be useful for breeders interested in developing BT-type japonica hybrids.

  6. Evidence for the introduction of the Asian red alga Neosiphonia japonica and its introgression with Neosiphonia harveyi (Ceramiales, Rhodophyta) in the Northwest Atlantic.

    Science.gov (United States)

    Savoie, Amanda M; Saunders, Gary W

    2015-12-01

    There is currently conflict in the literature on the taxonomic status of the reportedly cosmopolitan species Neosiphonia harveyi, a common red alga along the coast of Atlantic Canada and New England, USA. Neosiphonia harveyi sensu lato was assessed using three molecular markers: COI-5P, ITS and rbcL. All three markers clearly delimited three genetic species groups within N. harveyi sensu lato in this region, which we identified as N. harveyi, N. japonica and Polysiphonia akkeshiensis (here resurrected from synonymy with N. japonica). Although Neosiphonia harveyi is considered by some authors to be introduced to the Atlantic from the western Pacific, it was only confirmed from the North Atlantic suggesting it is native to this area. In contrast, Neosiphonia japonica was collected from only two sites in Rhode Island, USA, as well as from its reported native range in Asia (South Korea), which when combined with data in GenBank indicates that this species was introduced to the Northwest Atlantic. The GenBank data further indicate that N. japonica was also introduced to North Carolina, Spain, Australia and New Zealand. Despite the fact that all three markers clearly delimited N. harveyi and N. japonica as distinct genetic species groups, the ITS sequences for some N. harveyi individuals displayed mixed patterns and additivity indicating introgression of nuclear DNA from N. japonica into N. harveyi in the Northwest Atlantic. Introgression of DNA from an introduced species to a native species (i.e. 'genetic pollution') is one of the possible consequences of species introductions, and we believe this is the first documented evidence for this phenomenon in red algae. © 2015 John Wiley & Sons Ltd.

  7. Effects of the Brown Seaweed Laminaria japonica Supplementation on Serum Concentrations of IgG, Triglycerides, and Cholesterol, and Intestinal Microbiota Composition in Rats

    Directory of Open Access Journals (Sweden)

    Jae-Young Kim

    2018-04-01

    Full Text Available The intestinal microbial communities play critical roles in various aspects of body function of the host. Prebiotics, such as dietary fiber, can affect health of the host by altering the composition of intestinal microbiota. Although brown seaweed Laminaria japonica is rich in dietary fiber, studies on its prebiotic potential are quite rare. In this study, basal diet (control, basal diet supplemented with dried L. japonica (DLJ, heat-treated dried L. japonica (HLJ, or heated dried L. japonica with added fructooligosaccharide (FHLJ was fed to rats for 16 weeks. Serum concentrations of IgG, triglyceride, and cholesterol were measured. In addition, the intestinal microbiota composition was analyzed by high-throughput sequencing of 16S rRNA gene. As compared to the control group, DLJ, HLJ, and FHLJ groups showed significantly higher serum IgG concentration, but had lower weight gain and serum triglyceride concentration. Moreover, DLJ, HLJ, and FHLJ groups showed lower Fimicutes to Bacteroidetes ratio when compared with the control group. As compared with the control group, obesity-associated bacterial genera (Allobaculum, Turicibacter, Coprobacillus, Mollicute, and Oscilibacter, and the genera with pathogenic potentials (Mollicute, Bacteroides, Clostridium, Escherichia, and Prevotella decreased while leanness-associated genera (Alistipes, Bacteroides, and Prevotella, and lactic acid bacterial genera (Subdoligranulum, Streptococcus, Lactobacillus, Enterococcus, and Bifidobacterium increased in all treatment groups. On the contrary, butyric acid producing genera including Subdoligranulum, Roseburia, Eubacterium, Butyrivibrio, and Anaerotruncus increased significantly only in FHLJ group. The overall results support multiple prebiotic effects of seaweed L. japonica on rats as determined by body weight reduction, enhanced immune response, and desirable changes in intestinal microbiota composition, suggesting the great potential of L. japonica as an

  8. Gene expression profiles deciphering rice phenotypic variation between Nipponbare (Japonica and 93-11 (Indica during oxidative stress.

    Directory of Open Access Journals (Sweden)

    Fengxia Liu

    Full Text Available Rice is a very important food staple that feeds more than half the world's population. Two major Asian cultivated rice (Oryza sativa L. subspecies, japonica and indica, show significant phenotypic variation in their stress responses. However, the molecular mechanisms underlying this phenotypic variation are still largely unknown. A common link among different stresses is that they produce an oxidative burst and result in an increase of reactive oxygen species (ROS. In this study, methyl viologen (MV as a ROS agent was applied to investigate the rice oxidative stress response. We observed that 93-11 (indica seedlings exhibited leaf senescence with severe lesions under MV treatment compared to Nipponbare (japonica. Whole-genome microarray experiments were conducted, and 1,062 probe sets were identified with gene expression level polymorphisms between the two rice cultivars in addition to differential expression under MV treatment, which were assigned as Core Intersectional Probesets (CIPs. These CIPs were analyzed by gene ontology (GO and highlighted with enrichment GO terms related to toxin and oxidative stress responses as well as other responses. These GO term-enriched genes of the CIPs include glutathine S-transferases (GSTs, P450, plant defense genes, and secondary metabolism related genes such as chalcone synthase (CHS. Further insertion/deletion (InDel and regulatory element analyses for these identified CIPs suggested that there may be some eQTL hotspots related to oxidative stress in the rice genome, such as GST genes encoded on chromosome 10. In addition, we identified a group of marker genes individuating the japonica and indica subspecies. In summary, we developed a new strategy combining biological experiments and data mining to study the possible molecular mechanism of phenotypic variation during oxidative stress between Nipponbare and 93-11. This study will aid in the analysis of the molecular basis of quantitative traits.

  9. Carbon and nitrogen dynamics of the intertidal seagrass, Zostera japonica, on the southern coast of the Korean peninsula

    Science.gov (United States)

    Kim, Jong-Hyeob; Kim, Seung Hyeon; Kim, Young Kyun; Lee, Kun-Seop

    2016-12-01

    Seagrasses require a large amount of nutrient assimilation to support high levels of production, and thus nutrient limitation for growth often occurs in seagrass habitats. Seagrasses can take up nutrients from both the water column and sediments. However, since seagrasses inhabiting in the intertidal zones are exposed to the air during low tide, the intertidal species may exhibit significantly different carbon (C) and nitrogen (N) dynamics compared to the subtidal species. To examine C and N dynamics of the intertidal seagrass, Zostera japonica, C and N content and stable isotope ratios of above- and below-ground tissues were measured monthly at the three intertidal zones in Koje Bay on the southern coast of Korea. The C and N content and stable isotope (δ13C and δ15N) ratios of seagrass tissues exhibited significant seasonal variations. Both leaf and rhizome C content were not significantly correlated with productivity. Leaf δ13C values usually exhibited negative correlations with leaf productivity. These results of tissue C content and δ13C values suggest that photosynthesis of Z. japonica in the study site was not limited by inorganic C supply, and sufficient inorganic C was provided from the atmosphere. The tissue N content usually exhibited negative correlations with leaf productivity except at the upper intertidal zone, suggesting that Z. japonica growth was probably limited by N availability during high growing season. In the upper intertidal zone, no correlations between leaf productivity and tissue elemental content and stable isotope ratios were observed due to the severely suppressed growth caused by strong desiccation stress.

  10. Microtropins Q-W, ent-Labdane Glucosides: Microtropiosides G-I, Ursane-Type Triterpene Diglucoside and Flavonol Glycoside from the Leaves of Microtropis japonica.

    Science.gov (United States)

    Terazawa, Saori; Uemura, Yuka; Koyama, Yuka; Kawakami, Susumu; Sugimoto, Sachiko; Matsunami, Katsuyoshi; Otsuka, Hideaki; Shinzato, Takakazu; Kawahata, Masatoshi; Yamaguchi, Kentaro

    2017-01-01

    Microtropins Q-W, (2S,3R)-2-ethyl-2,3-dihydroxybutyrate of various glucosides and glucose, as well as three ent-labdane diterpenoid glucosides, named microtropiosides G, H and I, an ursane-type triterpene diglucoside and a flavonoid glycoside were isolated from the MeOH extract of the leaves of Microtropis japonica. The structure of microtropioside A, also isolated from the branches of M. japonica, was elucidated spectroscopically in a previous experiment and was found to possess a rare seven-membered oxyrane ring. Its structure was confirmed by X-ray crystallographic analysis of its pentaacetate.

  11. Accumulation of 137Cs in trefoil (leaf and stem), ''Mitsuba'', Cryptotaenia japonica Hassk, immersed in hydroponic culture medium

    International Nuclear Information System (INIS)

    Motegi, Misako; Miyake, Sadaaki; Ohsawa, Takashi; Nakazawa, Kiyoaki; Izumo, Yoshiro

    1998-01-01

    Accumulation of 137 Cs in trefoil (leaf and stem), ''Mitsuba'', Cryptotaenia japonica Hassk, with or without root was investigated to prepare higher radioactive plant in hydroponic culture medium (140-150 Bq/ml). It was found that 137 Cs concentration in plant tissue was increased with time, as high as 1.6 times of that in the culture medium after 4 days. On the other hand, 137 Cs concentration was affected by carrier element (Cs>6 ppm) and coexistent elements in the medium. Radioactivity of the plant after 4 days was shown to be sufficient for successive experiments. (author)

  12. Accumulation of {sup 137}Cs in trefoil (leaf and stem), ``Mitsuba``, Cryptotaenia japonica Hassk, immersed in hydroponic culture medium

    Energy Technology Data Exchange (ETDEWEB)

    Motegi, Misako; Miyake, Sadaaki; Ohsawa, Takashi; Nakazawa, Kiyoaki [Saitama Institute of Public Health, Urawa (Japan); Izumo, Yoshiro

    1998-11-01

    Accumulation of {sup 137}Cs in trefoil (leaf and stem), ``Mitsuba``, Cryptotaenia japonica Hassk, with or without root was investigated to prepare higher radioactive plant in hydroponic culture medium (140-150 Bq/ml). It was found that {sup 137}Cs concentration in plant tissue was increased with time, as high as 1.6 times of that in the culture medium after 4 days. On the other hand, {sup 137}Cs concentration was affected by carrier element (Cs>6 ppm) and coexistent elements in the medium. Radioactivity of the plant after 4 days was shown to be sufficient for successive experiments. (author)

  13. Comparative antimicrobial, phytotoxic and heamaglutination potential of eriobotrya japonica leaf extract and its zinc nano-particles

    International Nuclear Information System (INIS)

    Aziz, A.; Khan, I.; Azam, S.; Mehnaz, S.; Ahmad, B.

    2017-01-01

    The aim of the current study was to synthesize Zinc nanoparticles (ZnNPs) of Eriobotrya japonica, their characterization using standard procedures and its screening for various pharmacological activities; antibacterial, antifungal, phytotoxic and heamaglutination in comparison with the methanolic, ethanolic and Ethyl acetate (EtOAc) fractions. The UV-Visible absorption spectra of ZnNPs showed peaks at 455 nm and Fourier Transform Infrared spectroscopy (FTIR) confirmed the presence of various functional groups such as, hydroxyl, amide, carbonyl, aldehyde, and alkynes. The Scanning Electron Microscope (SEM) analysis showed that the particle size was between 30-60 nm with spherical shape. The presence of Zinc along with other elements, such as C, O, S, Si, and K, was confirmed by Energy Dispersive X-ray (EDX), andthe mean size was 33nm as indicated in X-ray diffraction (XRD) spectra. The results of percent antibacterial activity for the methanolic, ethanolic and EtOAc fractions of E. japonica against E. coli was 62, 59.2, 44.4, P. aeruginosa 44.4, 40.7, 37, S. aureus 69.2, 46.1, 50, A. baumannii 52.1, 43.4, 0, M. morganii 59, 40.9, 68, MRSA 73.6, 52.6, 0 and P. vulgaris 60, 52, 64. The Zn NPs exhibited significant antibacterial activity against MRSA (94%) and P. vulgaris (92%), good against S. aureus (73%) and M. morganii (63.6%), moderate against E. coli (48.1%) and A. baumannii (43.4%) and low against P. aeruginosa (37%), respectively. The methanolic, ethanolic and EtOAc fractions of E. japonica showed low (<24%) and no antifungal activity against the tested fungal pathogens. The antifungal activity of Zn NPs against the test fungal pathogens was; A. parasiticus (30%), P. notatum and A. niger (40%), P. chrysogenum (55%), H. pseudocrispula (60%) while it was inactive against V. longisporum. The methanolic, ethanolic and EtOAc extract of E. japonica showed 50, 50 and 60% growth regulation at 1000, 50, 40 and 60% at 100 µg/ml, while at 10 µg/ml, 40, 20 and 50

  14. Swimming depth of migrating silver eels Anguilla japonica released at seamounts of the West Mariana Ridge, their estimated spawning sites

    OpenAIRE

    Aoyama, J.; Hissmann, Karen; Yoshinaga, T.; Sasai, S.; Uto, T.; Ueda, H.

    1999-01-01

    Five hormone-treated female Japanese silver eels Anguilla japonica were tagged with ultrasonic transmitters and released by submersible in the West Pacific at seamounts of the West Mariana Ridge, their supposed spawning grounds. Four eels were tracked for 60 to 423 min in the vicinity of the seamounts. They did not settle at the seamounts but swam at a mean speed of 0.37 m s-1 into open water above deep ground. Their mean swimming depth ranged from 81 to 172 m. Experiments suggest that pre-ma...

  15. Autoestima en adolescentes con bajo rendimiento escolar a través del psicodiagnóstico de Rorschach

    Directory of Open Access Journals (Sweden)

    Magally de Garrido-Lecca

    1997-12-01

    Full Text Available El objetivo fue determinar si existen diferencias en la autoestima de adolescentes con bajo rendimiento escolar comparados con los de un rendimiento promedio o superior. Los participantes fueron 60 adolescentes, entre 13 y 16 años, de ambos sexos, con un C.I. promedio o superior al promedio, de los cuales 30 presentaban un bajo rendimiento escolar  (grupo de estudio y 30 un buen rendimiento (grupo contraste. Se administró el WISCR para determinar el C.I. y el Rorschach según el SC de Exner para evaluar la autoestima. Los resultados mostraron que el Rorschach es adecuado para evaluar la autoestima. Asimismo existe una diferencia significativa en la autoestima de adolescentes con bajo rendimiento como resultado de sus déficits afectivos, en comparación con los de alto rendimiento.

  16. Vaccination of bovines against Schistosomiasis japonica with highly irradiated schistosomula in China

    International Nuclear Information System (INIS)

    Hsue, S.Y.; Xu, S.T.; He, Y.X.; Shi, F.H.; Shen, W.; Hsue, H.F.; Osborne, J.W.; Clarke, W.R.

    1984-01-01

    Vaccination of Chinese bovines (cattle and buffaloes) against Schistosomiasis japonica with 36 kR gamma-irradiated schistosomula was done for laboratory challenge and for field trials in China. Altogether, 61 bovines were used. All experimental animals were vaccinated 2-3 times with 10,000 irradiated schistosomula per time. For the laboratory challenge, all experimental and control cattle were challenged with 500 normal cercariae and each buffalo, with 2,000 cercariae. The laboratory-challenged bovines were killed after 54-57 days of challenge; the bovines for the field trial in the lightly endemic area, after 5 months in the field; and the bovines for the field trial in the heavily endemic area, after 58-63 days. When the animals were killed, the number of mature worms in the vaccinated (experimental) and non-vaccinated (control) animals was recorded and the percentage of worm reduction in each group was calculated. The first group, consisting of three vaccinated and three non-vaccinated cattle, was given a laboratory challenge; the worm reduction was 71.6%. The second group, consisting of two vaccinated and three non-vaccinated buffaloes, was also given a laboratory challenge; the worm reduction was 74.4%. The third group, consisting of seven vaccinated and eight non-vaccinated buffaloes, was utilized in a field trial in a lightly endemic area; the worm reduction was 75.6%. The fourth group, consisting of eight vaccinated and nine non-vaccinated cattle, and the fifth group, consisting of nine vaccinated and nine non-vaccinated buffaloes, were pastured in a heavily endemic area. The worm reduction was 65.1% in the fourth group and 75.7% in the fifth group

  17. Memory-enhancing effects of Cuscuta japonica Choisy via enhancement of adult hippocampal neurogenesis in mice.

    Science.gov (United States)

    Moon, Minho; Jeong, Hyun Uk; Choi, Jin Gyu; Jeon, Seong Gak; Song, Eun Ji; Hong, Seon-Pyo; Oh, Myung Sook

    2016-09-15

    It is generally accepted that functional and structural changes within the hippocampus are involved in learning and memory and that adult neurogenesis in this region may modulate cognition. The extract of Cuscuta japonica Choisy (CJ) is a well-known traditional Chinese herbal medicine that has been used since ancient times as a rejuvenation remedy. The systemic effects of this herb are widely known and can be applied for the treatment of a number of physiological diseases, but there is a lack of evidence describing its effects on brain function. Thus, the present study investigated whether CJ would enhance memory function and/or increase hippocampal neurogenesis using mice orally administered with CJ water extract or vehicle for 21days. Performance on the novel object recognition and passive avoidance tests revealed that treatment with CJ dose-dependently improved the cognitive function of mice. Additionally, CJ increased the Ki-67-positive proliferating cells and the number of doublecortin-stained neuroblasts in the dentate gyrus (DG) of the hippocampus, and double labeling with 5-bromo-2-deoxyuridine and neuronal specific nuclear protein showed that CJ increased the number of mature neurons in the DG. Finally, CJ resulted in the upregulated expression of neurogenic differentiation factor, which is essential for the maturation and differentiation of granule cells in the hippocampus. Taken together, the present findings indicate that CJ stimulated neuronal cell proliferation, differentiation, and maturation, which are all processes associated with neurogenesis. Additionally, these findings suggest that CJ may improve learning and memory via the enhancement of adult hippocampal neurogenesis. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Tumor cell proliferation and cyclooxygenase inhibitory constituents in horseradish (Armoracia rusticana) and Wasabi (Wasabia japonica).

    Science.gov (United States)

    Weil, Marvin J; Zhang, Yanjun; Nair, Muraleedharan G

    2005-03-09

    Cyclooxygenase and human tumor cell growth inhibitory extracts of horseradish (Armoracia rusticana) and wasabi (Wasabia japonica) rhizomes upon purification yielded active compounds 1-3 from horseradish and 4 and 5 from wasabi rhizomes. Spectroscopic analyses confirmed the identities of these active compounds as plastoquinone-9 (1), 6-O-acyl-beta-d-glucosyl-beta-sitosterol (2), 1,2-dilinolenoyl-3-galactosylglycerol (3), linolenoyloleoyl-3-beta-galactosylglycerol (4), and 1,2-dipalmitoyl-3-beta-galactosylglycerol (5). 3-Acyl-sitosterols, sinigrin, gluconasturtiin, and phosphatidylcholines isolated from horseradish and alpha-tocopherol and ubiquinone-10 from wasabi rhizomes isolated were inactive in our assays. At a concentration of 60 microg/mL, compounds 1 and 2 selectively inhibited COX-1 enzyme by 28 and 32%, respectively. Compounds 3, 4, and 5 gave 75, 42, and 47% inhibition of COX-1 enzyme, respectively, at a concentration of 250 microg/mL. In a dose response study, compound 3 inhibited the proliferation of colon cancer cells (HCT-116) by 21.9, 42.9, 51.2, and 68.4% and lung cancer cells (NCI-H460) by 30, 39, 44, and 71% at concentrations of 7.5, 15, 30, and 60 microg/mL, respectively. At a concentration of 60 microg/mL, compound 4 inhibited the growth of colon, lung, and stomach cancer cells by 28, 17, and 44%, respectively. This is the first report of the COX-1 enzyme and cancer cell growth inhibitory monogalactosyl diacylglycerides from wasabi and horseradish rhizomes.

  19. Variation in organotin accumulation in relation to the life history in the Japanese eel Anguilla japonica

    Science.gov (United States)

    Ohji, Madoka; Harino, Hiroya; Arai, Takaomi

    2009-08-01

    In order to examine the ecological risks caused by organotin compounds (OTs) in diadromous fish migrating between sea and freshwaters, tributyltin (TBT) and triphenyltin (TPT) compounds and their breakdown products were determined in the catadromous eel Anguilla japonica, which has sea, estuarine and river life histories, collected in Japanese sea, brackish and freshwaters within the same region. Ontogenic changes in otolith strontium (Sr) and calcium (Ca) concentrations were examined along the life history transect to discriminate the migration type. There were generally three different patterns, which were categorized as 'sea eels', 'estuarine eels' and 'river eels' according to the otolith Sr:Ca ratio. The concentrations of TBT in silver eels (mature eels) were significantly higher than that in yellow eels (immature eels), and the percentages of TBT were also higher in silver eels than in yellow eels. A positive correlation was found between TBT concentration and the gonad-somatic index (GSI). It is thus considered that silver eels have a higher risk of contamination by TBT than yellow eels. TBT and TPT concentrations in sea eels were significantly higher than those in river eels, while no significant differences were observed in TBT and TPT concentrations in estuarine eels compared to sea and river eels. These results suggest that sea eels have a higher ecological risk of OT contamination than river eels during their life history, and the risk of OTs in estuarine eels is considered to be intermediate between that of sea and river eels. Positive linear relationships were found between Sr:Ca ratios and the concentrations of TBT and TPT. Therefore, these results suggest that the ecological risk of OTs increase as the sea residence period in the eel becomes longer. TBT and TPT concentrations in sea eels were significantly higher than those in river eels even at the same growth stage. Thus, it is clear that migratory type is the most important factor for OT

  20. Sensitivity of spore germination and germ tube elongation of Saccharina japonica to metal exposure.

    Science.gov (United States)

    Han, Taejun; Kong, Jeong-Ae; Kang, Hee-Gyu; Kim, Seon-Jin; Jin, Gyo-Sun; Choi, Hoon; Brown, Murray T

    2011-11-01

    The sensitivity of early life stages of the brown seaweed Saccharina japonica to six metals (Cd, Cu, Hg, Ni, Pb, Zn) and two waste-water samples were investigated and a new toxicity bioassay developed. The two endpoints used were spore germination and germ tube elongation with an exposure time of 24 h. Optimal test conditions determined for photon irradiance, pH, salinity and temperature were darkness, pH 8, 35‰ and 15°C, respectively. The toxicity ranking of five metals was: Hg (EC(50) of 41 and 42 μg l(-1)) > Cu (120 and 81 μg l(-1)) > Ni (2,009 and 1,360 μg l(-1)) > Zn (3,024 and 3,897 μg l(-1)) > Pb (4,760 and 4,429 μg l(-1)) > Cd (15,052 and 7,541 μg l(-1)) for germination and germ tube elongation, respectively. The sensitivities to Cd, Cu and Ni were greater in germ tube elongation than in germination process. When tested against two different waste-water samples (processed animal and printed circuit board waste-water) values of EC(50) were between 21.29 and 32.02% for germination and between 5.33 and 8.98% for germ tube elongation. Despite differences in their chemical composition, the toxic effects of waste-water samples, as indicated by EC(50) values, did not differ significantly for the same endpoints. The CV range for both germination and germ tube elongation was between 4.61 and 37.69%, indicating high levels of precision of the tests. The results compare favourably with those from more established test procedures employing micro- and macroalgae. The advantages and potential limitations of the bioassay for the assessment of anthropogenic impacts on coastal ecosystems and commercial cultivation areas in near-shore environments are discussed.

  1. Five new triterpene saponins, polygalasaponins XXVIII-XXXII from the root of Polygala japonica Houtt.

    Science.gov (United States)

    Zhang, D; Miyase, T; Kuroyanagi, M; Umehara, K; Ueno, A

    1996-04-01

    Five new oleanane-type saponins, polygalasaponins XXVIII-XXXII, along with one known saponin, polygalasaponin XXIV, and one known acylated sucrose, tenuifoliside C, were isolated from the root of Polygala japonica. The structures of these new compounds were elucidated as 3-O-beta-D-glucopyranosyl pesenegenin 28-O-beta-D-xylopyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1-->2)-beta-D-fucopyranosyl ester, 3-O-beta-D-glucopyranosyl presenegenin 28-O-beta-D-galactopyranosyl (1-->5)-beta-D-apiofuranosyl (1-->4)-beta-D-xylopyranosyl (1-->4)-alpha-L-rhamno-pyranosyl (1-->2)-beta-D-fucopyranosyl ester, 3-O-beta-D-glucopyranosyl presenegenin 28-O-beta-D-galactopyranosyl (1-->4)-beta-D-xylopyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1-->2)-[4-O-p-methoxycinnamoyl]-[beta-D-glucopyranosyl (1-->3)]-beta-D-fucopyranosyl ester, 3-O-beta-D-glucopyranosyl presenegenin 28-O-alpha-L-arabinopyranosyl (1-->3)-beta-D-xylopyranosyl (1-->4)-[beta-D-apiofuranosyl (1-->3)]-alpha-L-rhamnopyranosyl (1-->2)-[4-O-3,4,5-trimethoxycinnamoyl]-beta-D-fucopyranosyl ester, 3-O-beta-D-glucopyranosyl persenegenin 28-O-alpha-L-arabinopyranosyl (1-->3)-beta-D-xylopyranosyl (1-->4)-[beta-D-apiofuranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->2)-[4-O-p-methoxycinnamoyl]-[alpha-L-rhamnopyranosyl (1-->3)-beta-D-fucopyranosyl ester, respectively, on the basis of spectroscopic and chemical evidence.

  2. Genome-wide association mapping of root traits in a japonica rice panel.

    Directory of Open Access Journals (Sweden)

    Brigitte Courtois

    Full Text Available Rice is a crop prone to drought stress in upland and rainfed lowland ecosystems. A deep root system is recognized as the best drought avoidance mechanism. Genome-wide association mapping offers higher resolution for locating quantitative trait loci (QTLs than QTL mapping in biparental populations. We performed an association mapping study for root traits using a panel of 167 japonica accessions, mostly of tropical origin. The panel was genotyped at an average density of one marker per 22.5 kb using genotyping by sequencing technology. The linkage disequilibrium in the panel was high (r(2>0.6, on average, for 20 kb mean distances between markers. The plants were grown in transparent 50 cm × 20 cm × 2 cm Plexiglas nailboard sandwiches filled with 1.5 mm glass beads through which a nutrient solution was circulated. Root system architecture and biomass traits were measured in 30-day-old plants. The panel showed a moderate to high diversity in the various traits, particularly for deep (below 30 cm depth root mass and the number of deep roots. Association analyses were conducted using a mixed model involving both population structure and kinship to control for false positives. Nineteen associations were significant at P<1e-05, and 78 were significant at P<1e-04. The greatest numbers of significant associations were detected for deep root mass and the number of deep roots, whereas no significant associations were found for total root biomass or deep root proportion. Because several QTLs for different traits were co-localized, 51 unique loci were detected; several co-localized with meta-QTLs for root traits, but none co-localized with rice genes known to be involved in root growth. Several likely candidate genes were found in close proximity to these loci. Additional work is necessary to assess whether these markers are relevant in other backgrounds and whether the genes identified are robust candidates.

  3. Importancia de los antecedentes maternos en el recién nacido bajo peso

    Directory of Open Access Journals (Sweden)

    Gil Vicente Sarmiento Brooks

    2000-10-01

    Full Text Available Se realizó un estudio observacional, analítico, con un modelo de diseño del tipo casos y controles en 117 mujeres del Policlínico Docente "La Rampa" perteneciente al municipio Plaza de la Revolución de las cuales 17 tuvieron un recién nacido con un peso inferior a los 2 500 g. Las restantes 100 mujeres lograron niños con un peso igual al anteriormente señalado o superior. Analizamos la influencia de algunos antecedentes como el orden de nacimientos y de embarazos, el número de abortos y las gestaciones de bajo peso anteriores y determinados antecedentes patológicos de la madre. Se concluyó que los factores que influyen en el nacimiento de un niño con bajo peso son, entre otros: que sea el primer nacimiento (RR = 2; el primer embarazo (RR = 1,8; el haber tenido 2 o más abortos previos (RR = 4,9, el antecedente de haber tenido uno o más recién nacido de bajo peso (RNBP (RR = 6,9 y ciertas enfermedades maternas como la urosepsis (RR = 3,7, la hipertensión crónica (RR = 3,21, la anemia (RR = 2,7 y el asma (RR = 1,9. Se hacen algunas recomendacionesAn observational and analytical case-control study was conducted among 117 women attended at "La Rampa" Teaching Polyclinic, in the municipality of Plaza de la Revolución. 17 of these women had a newborn infant with a birth weight under 2 500 g. The rest of them had children with the same weight or higher. The influence of some history as the order of births and pregnancies, the number of abortions and the previous low weight gestations and certain pathological history of the mother were analyzed. It was concluded that the factors that influenced on the birth of a low birth weight infant are, among others: the first birth (RR=2, the first pregnancy (RR=1,8; having had 2 or more previous abortions (RR=4,9, having had one or more low birth weight infants (RR=6,9, and some maternal diseases as urosepsis (RR=3,7, chronic hypertension (RR=3,21, anemia (RR=2,7 and asthma (RR=1,9

  4. Situación General de la Industria de la Construcción de Bajo Bordo

    OpenAIRE

    Chui, Alexander; Gamero, Magali; Cornejo, Karla; Rios, Vanessa; Valdivia, Maria Laura; Benzaquén de las Casas, Jorge

    2016-01-01

    Objetivo: Determinar las condiciones en que se encuentra la industria de construcción naval de bajo bordo en el Perú, relacionado con su contexto latinoamericano. Métodos: Se estableció un protocolo de actuación a base entrevistas, cuestionarios y recopilación de datos con el fin de conocer los principales indicadores de la dinámica funcional e industria de construcción naval de bajo bordo. Resultados: El Perú ha crecido notablemente en las últimas dos décadas como consecuencia de la apertura...

  5. Comportamiento Tribológico de Aluminio Sinterizado y Desgastado Bajo Distintas Condiciones Comportamiento Tribológico de Aluminio Sinterizado y Desgastado Bajo Distintas Condiciones

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    Veridiana Reyes Z.

    2012-02-01

    Full Text Available Wear is one of the most costly damages in industry and research has been directed towards its prevention. This paper deals with the behavior of aluminum pieces, prepared by powder metallurgy, under wear. The samples tested were prepared under different processing conditions (compacting pressure, temperature and sintering time. The wear test was carried out ina pin-in-disc machine, at various rates and wear pressures. The results showed the tribological behavior of aluminum. Also, a comparative study between a 356-aluminium alloy and pure aluminum was carried out, showing the feasibility of attaining similar tribological behavior between a conventional alloy and pure aluminum prepared by powder metallurgy techniques.El desgaste es uno de los daños más costosos para la industria y las investigaciones buscan entender los mecanismos del desgaste que podrían disminuirlo o evitarlo. Este trabajo analiza el comportamiento del aluminio obtenido por metalurgia de polvos cuando se somete a desgaste. Las muestras usadas fueron preparadas bajo diversas condiciones de proceso (presión de la compactación, la temperatura y tiempode tratamiento térmico. Las pruebas de desgaste se realizaron en una máquina de pin-en-disco y fueron probados diferentes valores de velocidad y presión de desgaste. Los resultados obtenidos muestran el comportamiento tribológico del aluminio. Se presenta también un estudio comparativo entre una aleación de aluminio 356 y aluminio puro, demostrándose la viabilidad de lograr un comportamiento tribológico similar entre esta aleación convencional y el aluminio puro preparado por metalurgia de polvos.

  6. Evaluation of the ORYZA2000 Rice Growth Model under Nitrogen-Limited Conditions in an Irrigated Mediterranean Environment Evaluación del Modelo de Crecimiento de Arroz ORYZA2000 bajo Condiciones Limitadas de Nitrógeno en un Ambiente Mediterráneo Regado

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    Pamela Artacho

    2011-03-01

    Full Text Available ORYZA2000 is a growth model for tropical lowland rice ( Oryza sativa L. developed by the International Rice Research Institute and Wageningen University. This model has been evaluated extensively in a wide range of environments. However, reports examining japonica cultivars growing in temperate climates are scarce. In this study, ORYZA2000 was calibrated and evaluated using data from experiments carried out in the South-Central area of Chile. These experiments were performed on a japonica rice cultivar growing under an irrigated Mediterranean environment at various N rates. ORYZA2000 was then applied to explore potential yield and grain yield response to N fertilization under likely weather conditions in the major rice-producing area in Chile. ORYZA2000 was sufficiently accurate to simulate grain yield and crop N uptake at the end of the season. Final crop N uptake was simulated with a root mean squared error (RMSE of 20 kg ha-1 (15% and grain yield with a RMSE of 1666 kg ha-1 (19%. However, the prediction of biomass and N uptake of individual organs throughout the season was poor. A long-term simulation study confirmed a potential yield as high as 12 000 kg ha-1 in the Parral area, as well as the existence of a scope for yield increase. The yield response to N fertilization was predicted even at rates of 300 kg ha-1, although a significant probability of low yields was also observed. This trend supports the need to incorporate dynamic N management in Chilean rice production.ORYZA2000 es un modelo para arroz tropical regado desarrollado por el Instituto Internacional de Investigación en Arroz y Universidad de Wageningen, que ha sido evaluado en un amplio rango de ambientes. Sin embargo, los reportes para cultivares japónica creciendo en ambientes templados son escasos. En este estudio, ORYZA2000 fue calibrado y evaluado usando datos de experimentos realizados en el centro-sur de Chile, que incluyeron una variedad japónica cultivada bajo un

  7. Sobre la toma de decisiones económicas bajo incertidumbre

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    Aurora García Gallego

    2012-04-01

    Full Text Available En este artículo resumimos la evolución de la Teoría Económica sobre la toma de decisiones bajo incertidumbre. Tras una breve introducción a la teoría de la Utilidad Esperada, nos referimos a comportamientos paradójicos que obligaron a los economistas a adoptar teorías menos simplistas que permitieran explicar un mayor abanico de fenómenos. La mayor complejidad conlleva una descripción multidimensional de las actitudes humanas frente al riesgo económico. Sin embargo, las mediciones de la actitud frente al riesgo utilizadas por los economistas continúan siendo, en su mayoría, unidimensionales.

  8. Vernacular modernism and melodramatic imagination in Bajo el cielo antioqueño

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    Álvaro Andrés Villegas

    2016-12-01

    Full Text Available This article focuses on the film “Bajo el cielo antioqueño”, directed by Arturo Acevedo Vallarino and released in 1925. First, the collective efforts of the Medellin privileged sectors are described, which participated as investors and as actors. Then, the movie’s appropriation of what could be called the “vernacular modernism” is analyzed through the cultural matrix of melodrama, which is characterized by intensity and schematization of feelings, by the story’s development from ignorance to recognition, the supremacy of family relationships and essential loyalties, as well as by the expression of emotional excess. Finally, the acceptance of the movie by the audience, which was characterized by the consensus towards the regional representation shown in the movie, will be commented on.

  9. Desarrollo postembrionario de Ronderosia bergi (Orthoptera: Acrididae: Melanoplinae bajo condiciones controladas

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    Yanina MARIOTTINI

    2006-01-01

    Full Text Available Mediante el seguimiento de cohortes correspondientes a la primera generación de bioterio, provenientes de ejemplares capturados en el sudeste de la provincia de San Luis, se registraron algunos parámetros biológicos fundamentales del acridio Ronderosia bergi (Stål bajo condiciones controladas (30°C, 40% HR; 14hs. luz, 10 hs. oscuridad. La duración total del desarrollo postembrionario fue de 213 días, de los cuales 30 correspondieron a los cinco estadios del desarrollo ninfal. El número promedio de huevos por postura fue de 16,8, y la madurez sexual fue alcanzada dentro de los 4-5 días después del ingreso a imago. Las diferencias con los escasos datos provenientes de estudios previos, obedecerían a las distintas condiciones de crianza o a una alta variabilidad intraespecífica.

  10. Bajo peso al nacer y su implicación en el desarrollo psicomotor

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    Doris Amparo Parada-Rico

    2015-12-01

    Full Text Available Los niños que al nacer presentan peso bajo, pueden desarrollar graves problemas de salud, así mismo, el riesgo de sufrir problemas e incapacidades en su desarrollo psicomotor a largo plazo es mayor. En la revisión en bases de datos científicas, se identifica como un problema de salud a escala mundial, puesto que estos recién nacidos pueden tener serias limitaciones en su supervivencia y posteriormente en su calidad de vida; por ende, se debe evaluar minuciosamente su desarrollo psicomotor, de manera que se asegure la detección temprana de factores de riesgo susceptibles a intervenciones de cuidado.

  11. Comportamiento del fruto de uchuva Physalis peruviana L., bajo diferentes temperaturas de almacenamiento

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    Elberth Hernando Pinzón

    2015-12-01

    Full Text Available El fruto de la uchuva Physalis peruviana L. es muy apetecido en los mercados internacionales, por sus características nutracéuticas; sin embargo, por ser un fruto climatérico, los procesos de maduración se dan de forma acelerada disminuyendo considerablemente su vida poscosecha. Teniendo en cuenta lo anterior se han desarrollado diferentes técnicas que permiten desacelerar los procesos normales de maduración, entre las que se encuentran, la modificación de atmósferas o el almacenamiento a diferentes temperaturas, siendo éste último, muy utilizado por la facilidad de manejo y bajo costo. Por lo anterior, se evaluó el comportamiento del fruto de uchuva ecotipo Colombia sometido a diferentes temperaturas de almacenamiento, a los que se les evaluó la pérdida de masa fresca, índice de color, firmeza, sólidos solubles totales y acidez total. Se presentaron diferencias significativas para todas las variables evaluadas entre los tratamientos bajo refrigeración y el testigo. La temperatura de almacenamiento, influyó sobre todas las características físicas y químicas evaluadas de manera satisfactoria, ya que luego de 18 días después de almacenamiento los frutos refrigerados conservaron su calidad, siendo favorable para su comercialización; sin embargo, se espera en estudios futuros realizar pruebas de vida en anaquel que permitan descartar fisiopatías que afecten la presentación del producto así como su consumo.

  12. New data about the archaeological area of Marroquíes Bajos: the fifth ditch

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    Sánchez, Alberto

    2005-12-01

    Full Text Available The present paper presents the results that were obtained in the excavation carried out in the plot DOC-1 (SUNP-1 in the Archaeological Area of Marroquíes Bajos. The main contribution of this excavation in the context of all the research done in this archaeological area has to do with its Chalcolithic phase, in particular with the delimitation and documentation of the fifth ditch in an extension that was hitherto unknown. Along with this ditch, a fortified structure was also documented, which consisted of two walls and a semicircular hollow tower which was very likely to protect an entrance. Finally, the research has yielded the first data on the food and health of the people in this settlement by analysing a set of bone remains located on the bottom of the ditch.

    Este trabajo presenta los resultados obtenidos en la excavación realizada en la parcela DOC-1 (SUNP-1 de la Zona Arqueológica de Marroquíes Bajos. La principal aportación de la excavación al ámbito de las investigaciones desarrolladas en esta zona arqueológica se centra en su fase calcolítica, concretamente en la delimitación y documentación del quinto foso en una extensión no conocida hasta el momento. Asociado este foso se documentó igualmente una estructura fortificada compuesta por dos muros y una torre semicircular hueca que muy posiblemente protegía un acceso. Finalmente la investigación desarrollada a permitido obtener los primeros datos sobre alimentación y salud de la población del asentamiento mediante el análisis de un conjunto de restos óseos localizados en el fondo del foso.

  13. Distribution Characterization of Leaf and Hull Pubescences and Genetic Analysis of Their Numbers in japonica Rice (Oryza sativa

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    Xiao-biao ZHU

    2008-12-01

    Full Text Available Distributions of pubescences on leaf blade and hull in japonica rice were observed under an optical microscope. Numbers of leaf and hull pubescences in P1, P2, F1, B1, B2 and F2 generations were investigated in three combinations of japonica rice (Sidao 10A/Wuyujing 3R, Wuyujing 3A/Sidao 10R and Liuyan 189A/HR-122, and genetic analysis for these two traits were conducted by using the joint analysis method of P1, P2, F1, B1, B2 and F2 generations with the mixed major gene plus polygene inheritance models. Leaf pubescences characterized by swollen base and fine tip distributed regularly on the boundary between dark green stripe and light green stripe of leaf blade. Hull pubescences with various lengths distributed irregularly on the whole hull. Numbers of leaf pubescences in the reciprocal combinations of Sidao 10A/Wuyujing 3R and Wuyujing 3A/Sidao 10R and numbers of hull pubescences in all the three combinations were controlled by one pair of additive major genes plus additive-dominant polygenes. In the combination of Liuyan 189A/HR-122, number of leaf pubescences was controlled by one pair of additive-dominant major genes plus additive-dominant polygenes. Both numbers of leaf and hull pubescences were mainly governed by major genes.

  14. Wasabia japonica is a potential functional food to prevent colitis via inhibiting the NF-κB signaling pathway.

    Science.gov (United States)

    Kang, Ju-Hee; Choi, Seungho; Jang, Jeong-Eun; Ramalingam, Prakash; Ko, Young Tag; Kim, Sun Yeou; Oh, Seung Hyun

    2017-08-01

    Inflammatory bowel diseases (IBDs), including Crohn's disease (CD) and ulcerative colitis (UC), are prevalent and debilitating health problems worldwide. Many types of drugs are used to treat IBDs, but they exhibit adverse effects such as vomiting, nausea, abdominal pain, diarrhea, etc. In order to overcome the limitations of current therapeutic drugs, scientists have searched for functional foods from natural resources. In this study, we investigated the anti-colitic effects of Wasabia japonica extract in a DSS-induced colitis model. Wasabi japonica is a plant of the Brassicaceae family that has recently been reported to exhibit properties of detoxification, anti-inflammation, and induction of apoptosis in cancer cells. In this study, we generated wasabi ethanol extract (WK) and assessed its anti-colitic effect. In addition, in order to improve delivery of the extract to the colon, WK was coated with 5% Eudragit S100 (WKE), after which the anti-colitic effects of WKE were assessed. In conclusion, WK prevented development of colitis through inhibition of the NF-kB signaling pathway and recovery of epithelial tight junctions. In addition, the anti-colitic effect of WK was enhanced by improving its delivery to the colon by coating the WK with Eudragit S100. Therefore, we suggest that wasabi can be used as a new functional food to prevent IBDs due to its anti-colitic effect.

  15. Brown seaweed (Saccharina japonica) as an edible natural delivery matrix for allyl isothiocyanate inhibiting food-borne bacteria.

    Science.gov (United States)

    Siahaan, Evi Amelia; Pendleton, Phillip; Woo, Hee-Chul; Chun, Byung-Soo

    2014-01-01

    The edible, brown seaweed Saccharina japonica was prepared as powder in the size range 500-900 μm for the desorption release of allyl isothiocyanate (AITC). Powders were used as raw (containing lipids) and as de-oiled, where the lipid was removed. In general, de-oiled powders adsorbed larger masses of AITC after vapour or solution contact. Mass adsorbed due to solution contact exceeded vapour contact. Larger particles adsorbed more than smaller particles. No chemical bonding between AITC and the powder surface occurred. Release from vapour deposited particles reached 70-85% available within 72 h; solution deposited reached 70-90% available at 192 h. The larger amounts of AITC adsorbed via solution deposition resulted in greater vapour-phase concentrations at 72 h for antimicrobial activity studies. No loss of activity was detected against Escherichia coli, Salmonella Typhimurium or Bacillus cereus. Only a nominal activity against Staphylococcus aureus was demonstrated. S. japonica powder could be used as an edible, natural vehicle for AITC delivery. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. The Interactive Effects of Elevated CO2 and Ammonium Enrichment on the Physiological Performances of Saccharina japonica (Laminariales, Phaeophyta)

    Science.gov (United States)

    Kang, Jin Woo; Chung, Ik Kyo

    2018-04-01

    Environmental challenges such as ocean acidification and eutrophication influence the physiology of kelp species. We investigated their interactive effects on Saccharina japonica (Laminariales, Phaeophyta) under two pH conditions [Low, 7.50; High (control), 8.10] and three NH4 +concentrations (Low, 4; Medium, 60; High, 120 μM). The degree of variation of pH values in the culture medium and inhibition rate of photosynthetic oxygen evolution by acetazolamide were affected by pH treatments. Relative growth rates, carbon, nitrogen, and the C:N ratio in tissue samples were influenced by higher concentrations of NH4 + . Rates of photosynthetic oxygen evolution were enhanced under elevated CO2 or NH4 +conditions, independently, but these two factors did not show an interactive effect. However, rates of NH4 +uptake were influenced by the interactive effect of increased CO2 under elevated NH4 +treatment. Although ocean acidification and eutrophication states had an impact on physiological performance, chlorophyll fluorescence was not affected by those conditions. Our results indicated that the physiological reactions by this alga were influenced to some extent by a rise in the levels of CO2 and NH4 + . Therefore, we expect that the biomass accumulation of S. japonica may well increase under future scenarios of ocean acidification and eutrophication.

  17. Mass-spectrometry-directed analysis and purification of pyrrolizidine alkaloid cis/trans isomers in Gynura japonica.

    Science.gov (United States)

    Fang, Lianxiang; Xiong, Aizhen; Yang, Xiao; Cheng, Wenzhi; Yang, Li; Wang, Zhengtao

    2014-08-01

    Pyrrolizidine alkaloids are highly hepatotoxic natural chemicals that produce irreversible chronic and acute hepatotoxic effects on human beings. Purification of large amounts of pyrrolizidine alkaloids is necessary for toxicity studies. In this study, an efficient method for targeted analysis and purification of pyrrolizidine alkaloid cis/trans isomers from herbal materials was developed for the first time. Targeted analysis of the hepatotoxic pyrrolizidine alkaloids was performed by liquid chromatography with tandem mass spectrometry (precursor ion scan and daughter ion scan), and the purification of pyrrolizidine alkaloids was achieved with a mass-directed auto purification system. The extraction and preparative liquid chromatography conditions were optimized. The developed method was applied to analysis of Gynura japonica (Thunb.) Juel., a herbal medicine traditionally used for detumescence and relieving pain but is potentially hepatotoxic as it contains pyrrolizidine alkaloids. Twelve pyrrolizidine alkaloids (six cis/trans isomer pairs) were identified with reference compounds or characterized by liquid chromatography with tandem mass spectrometry, and five individual pyrrolizidine alkaloids, including (E)-seneciphylline, seneciphylline, integerrimine, senecionine, and seneciphyllinine, were prepared from G. japonica roots with high efficiency. The results of this work provide a new technique for the preparation of large amounts of pyrrolizidine alkaloid reference substances, which will also benefit toxicological studies of pyrrolizidine alkaloids and treatments for pyrrolizidine alkaloid-induced toxicity. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Temporal pattern in biometrics and nutrient stoichiometry of the intertidal seagrass Zostera japonica and its adaptation to air exposure in a temperate marine lagoon (China): Implications for restoration and management.

    Science.gov (United States)

    Zhang, Xiaomei; Zhou, Yi; Liu, Peng; Wang, Feng; Liu, Bingjian; Liu, Xujia; Yang, Hongsheng

    2015-05-15

    In coastal areas of China, the seagrass Zostera japonica has drastically decreased in the past decades. Swan Lake is an exception, where we found extensive areas of Z. japonica beds. The growth of Z. japonica in the lagoon exhibited strong seasonal variation. The maximum shoot density of 9880±2786 shoots m(-2) occurred in August. The maximum specific growth rate (SGR) of 4.99±1.99%⋅d(-1) was recorded in June 2012. SGR might be a good parameter for assessing the growth status of Z. japonica population. N and P contents in the rhizome were significantly lower than those in the leaf and leaf sheath. Lower C/P ratios suggested P enrichment of the seagrass. The occurrence of Z. japonica in Swan Lake was featured by adapting to the intertidal harsh environments. The transplantation method using sectioned rhizomes would be a potential way for restoration of degraded Z. japonica beds. The establishment of the Rongcheng Swan National Nature Reserve in China has contributed to the survival and expansion of Z. japonica in Swan Lake. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. CRISPR/Cas9-Mediated Gene Editing in Rice (Oryza sativa L. japonica cv. Katy) for Stable Resistance against Blast Fungus (Magnaporthe oryzae)

    Science.gov (United States)

    Rice blast is a recurring and devastating disease in the USA and worldwide. In the USA, the blast-resistance (R) genes found in a tropical japonica cultivar, Katy, reduce blast damages from 1990 to present. The cultivar is still used as a principal donor of blast R genes in developing numerous elit...

  20. DNA Methylation Influences Chlorogenic Acid Biosynthesis in Lonicera japonica by Mediating LjbZIP8 to Regulate Phenylalanine Ammonia-Lyase 2 Expression

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    Liangping Zha

    2017-07-01

    Full Text Available The content of active compounds differ in buds and flowers of Lonicera japonica (FLJ and L. japonica var. chinensis (rFLJ. Chlorogenic acid (CGAs were major active compounds of L. japonica and regarded as measurements for quality evaluation. However, little is known concerning the formation of active compounds at the molecular level. We quantified the major CGAs in FLJ and rFLJ, and found the concentrations of CGAs were higher in the buds of rFLJ than those of FLJ. Further analysis of CpG methylation of CGAs biosynthesis genes showed differences between FLJ and rFLJ in the 5′-UTR of phenylalanine ammonia-lyase 2 (PAL2. We identified 11 LjbZIP proteins and 24 rLjbZIP proteins with conserved basic leucine zipper domains, subcellular localization, and electrophoretic mobility shift assay showed that the transcription factor LjbZIP8 is a nuclear-localized protein that specifically binds to the G-box element of the LjPAL2 5′-UTR. Additionally, a transactivation assay and LjbZIP8 overexpression in transgenic tobacco indicated that LjbZIP8 could function as a repressor of transcription. Finally, treatment with 5-azacytidine decreased the transcription level of LjPAL2 and CGAs content in FLJ leaves. These results raise the possibility that DNA methylation might influence the recruitment of LjbZIP8, regulating PAL2 expression level and CGAs content in L. japonica.

  1. Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.

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    Amir Hossein Esmaeili

    2014-06-01

    Full Text Available Objectives: Eriobotrya japonica belongs to the Rosaceae. Studies have shown that the flowers of this plant are rich in phenolic and flavonoid compounds. Accorrdingly, the evaluation of antioxidative effects of Eriobotrya japonica Flower Extract (EJFE have been performed in vitro. Material and Methods: In this study, to investigate the influences of components of EJFE on its antioxidative activity, extract was prepared using hydro-alcoholic (25:75 V/V solvent and the antioxidative activity of the extract was evaluated based on the scavenging of various radicals (DPPH and H2O2 by spectrophotometric method and chelating of ferrous ions by ferrozine reagent. Results: HPLC analysis of the Eriobotrya japonica Flower Extract (EJFE revealed hesperetin and gallic acid as the major antioxidants. When the content of total flavonoid and polyphenolic compounds in the flower extract of this plant was examined, a significantly higher level of total polyphenols was found in Eriobotrya japonica flower extract. Conclusion: Results demonstrate that the high ability to scavenge free radicals, reducing power, and Fe+2chelating activity exerted by the EJFE were due to the high content of hesperetin and gallic acid in the flowers.

  2. The external morphology of the mouthparts, and observations on feeding and behavior of Tuckerella japonica on Camellia sinensis in the continental United States

    Science.gov (United States)

    Tuckerella japonica Ehara (Acari: Tetranychoidea: Tuckerellidae) is found where longitudinal splitting occurs on exposed green periderm tissue of shoots on certain varieties or seedling plants of Camellia sinensis (L.) O. Kuntze (Theales: Theaceae) in the continental United States. The mite is able ...

  3. Diurnal changes of net photosynthetic rate (NPR) in leaves of Lonicera japonica Thunb. and the responding mathematical model of NPR to photosynthetic valid radiation

    International Nuclear Information System (INIS)

    Wu Dafu; Zhang Shengli; Li Dongfang

    2009-01-01

    [Objective] The study provided theoretical basis for production practice . [Method] With Lonicera japonica Thunb .as material, diurnal changes of net photosynthetic rate (NPR) in leaves of the plant and the responding mathematical model of NPR to photosynthetic valid radiation were studied using portable photosynthetic determinator system. [Result] Like most of C3 plants, the diurnal changes curve of NPR of Lonicera japonica Thunb .showed double peaks, but there were time difference in reaching the peak value between the study and previous ones . The responding mathematical model of NPR to photosynthetic valid radiation could be described by three mathematic functions, such as logarithm, linearity and binomial, but binomial function was more precise than the others. Light saturation point of Lonicera japonica Thunb. was figured out by binomial equation deduced in the study , and light saturation point was 1 086 .3 μmol/ (m2•s) . [Conclusion] The diurnal changes curve of NPR of Lonicera japonica Thunb .showed double peaks, and the responding mathematical model of NPR to photosynthetic valid radiation could be described by binomial functions

  4. Has the rapidly expanding invasive dwarf eelgrass Zostera japonica in Yaquina estuary, Oregon impacted the distribution of native eelgrass Zostera marina – a critical intertidal habitat? - CERF

    Science.gov (United States)

    Native eelgrass, Zostera marina, occupies a significant portion of marine-dominated intertidal and near-subtidal sectors of many coastal estuaries. In recent decades an invasive congener, Z. japonica, has become established in many Pacific Northwest estuaries. We measured the h...

  5. Has the rapidly expanding invasive dwarf eelgrass Zostera japonica in Yaquina estuary, Oregon impacted the distribution of native eelgrass Zostera marina – a critical intertidal habitat?

    Science.gov (United States)

    Native eelgrass, Zostera marina, occupies a significant portion of marine-dominated intertidal and near-subtidal sectors of many coastal estuaries. In recent decades an invasive congener, Z. japonica, has become established in many Pacific Northwest estuaries. We measured the h...

  6. Comparison of non-indigenous dwarf eelgrass (Zostera japonica) and native eelgrass (Z. marina) distributions in a northeast Pacific estuary: 1997-2014

    Science.gov (United States)

    This study addressed the following question: In a coastal estuary of the northeastern Pacific Ocean with a relatively large areal extent of the native eelgrass Zostera marina, is an expanding distribution of the non-indigenous dwarf eelgrass Z. japonica accompanied by a measurab...

  7. A quick method for species identification of Japanese eel (Anguilla japonica) using real-time PCR: an onboard application for use during sampling surveys.

    Science.gov (United States)

    Watanabe, Shun; Minegishi, Yuki; Yoshinaga, Tatsuki; Aoyama, Jun; Tsukamoto, Katsumi

    2004-01-01

    To compensate for the limited number of morphological characteristics of fish eggs and larvae, we established a convenient and robust method of species identification for eggs of the Japanese eel (Anguilla japonica) using a real-time polymerase chain reaction (PCR) that can be performed onboard research ships at sea. A total of about 1.2 kbp of the mitochondrial 16S ribosomal RNA gene sequences from all species of Anguilla and 3 other anguilliform species were compared to design specific primer pairs and a probe for A. japonica. This real-time PCR amplification was conducted for a total of 44 specimens including A. japonica, A. marmorata, A. bicolor pacifica, and 6 other anguilliform species. Immediate PCR amplification was only observed in A. japonica. We then tested this method under onboard conditions and obtained the same result as had been produced in the laboratory. These results suggest that real-time PCR can be a powerful tool for detecting Japanese eel eggs and newly hatched larvae immediately after onboard sampling during research cruises and will allow targeted sampling efforts to occur rapidly in response to any positive onboard identification of the eggs and larvae of this species.

  8. Effects of temperature and light intensity on the uptake and assimilation of 15N-labeled ammonium and nitrate in Indica and Japonica rice plants

    International Nuclear Information System (INIS)

    Ta, T.C.; Ohira, Koji

    1982-01-01

    The effects of various environmental condition such as temperature and light intensity on the uptake and assimilation of ammonium and nitrate in 4-week-old Indica and Japonica rice plants were studied using the 15 N tracer technique. Both temperature and light intensity profoundly affected the uptake and assimilation of ammonium and nitrate, and the effects were more apparent in the utilization of nitrate by both Indica and Japonica rice plants. The uptake as well as assimilation of the two forms of nitrogen were greatly inhibited at low temperature and low light intensity. On the other hand, although no significant difference in the effects of environmental conditions on the utilization of ammonium was observed between the Indica and Japonica rice plants, the former were more sensitive than the latter in the utilization of nitrate. At high temperature and high light intensity, the Indica rice plants absorbed, reduced, and assimilated nitrate more effectively than the Japonica, and this effect disappeared when the temperature or light intensity was reduced. (author)

  9. Effects of salinity on photosynthesis and respiration of the seagrass Zostera japonica: A comparison of two established populations in North America

    Science.gov (United States)

    Zostera japonica is a non-native seagrass along the Pacific Coast of North America that is distributed from Northern California, USA to British Columbia, Canada. Recent observations indicate that the species is expanding both latitudinally and into areas of lower salinity. Ther...

  10. Identification of source and recharge zones in an aquifer system from water stable isotope. Case Study Bajo Cauca Antioqueno

    International Nuclear Information System (INIS)

    Palacio B, Paola Andrea; Betancur V, Teresita

    2008-01-01

    Hydrology and hydrogeochemical are auxiliary techniques to valid conceptual hydrogeology an recharge models. Stable isotopes from water trace sources and path flow and Tritium indicates age. This paper is about the use of D 18, D 2H y 3H to study the aquifer system on Bajo Cauca antioqueno

  11. Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

    Science.gov (United States)

    Wan, Peng; Yang, Xiaoman; Cai, Bingna; Chen, Hua; Sun, Huili; Chen, Deke; Pan, Jianyu

    2015-08-01

    In the present study, ultrasonic extraction technique (UET) is used to improve the yield of polysaccharides from Laminaria japonica (LJPs). And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design (BBD) combined with response surface methodology (RSM) is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80°C and ratio of material to solvent at 1:50 (g mL-1). Under these optimal ultrasonic extraction conditions, an actual experimental yield (5.75% ± 0.3%) is close to the predicted result (5.67%) with no significant difference ( P > 0.05). Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides (LJP) and two major ethanol precipitated fractions (LJP1 and LJP2) are in a concentration-dependent manner. LJP2 (30%-60% ethanol precipitated polysaccharides) possesses the strongest α-glucosidase inhibitory activity and moderate scavenging activity against hydroxyl radicals (66.09% ± 2.19%, 3.0 mg mL-1). Also, the inhibitory activity against α-glucosidase (59.08% ± 3.79%, 5.0 mg mL-1) is close to that of acarbose (63.99% ± 3.27%, 5.0 mg mL-1). LJP1 (30% ethanol precipitated polysaccharides) exhibits the strongest scavenging activity against hydroxyl radicals (99.80% ± 0.00%, 3.0 mg mL-1) and moderate α-glucosidase inhibitory activity (47.76% ± 1.92%, 5.0 mg mL-1). LJP shows the most remarkable DPPH scavenging activity (66.20% ± 0.11%, 5.0 mg mL-1) but weakest α-glucosidase inhibitory activity (37.77% ± 1.30%, 5.0 mg mL-1). However, all these LJPs exert weak inhibitory effects against α-amylase. These results show that UET is an effective method for extracting bioactive polysaccharides from seaweed materials. LJP1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of

  12. Plastid structure and carotenogenic gene expression in red- and white-fleshed loquat (Eriobotrya japonica) fruits

    Science.gov (United States)

    Fu, Xiumin; Kong, Wenbin; Peng, Gang; Zhou, Jingyi; Azam, Muhammad; Xu, Changjie; Grierson, Don; Chen, Kunsong

    2012-01-01

    Loquat (Eriobotrya japonica Lindl.) can be sorted into red- and white-fleshed cultivars. The flesh of Luoyangqing (LYQ, red-fleshed) appears red-orange because of a high content of carotenoids while the flesh of Baisha (BS, white-fleshed) appears ivory white due to a lack of carotenoid accumulation. The carotenoid content in the peel and flesh of LYQ was approximately 68 μg g−1 and 13 μg g−1 fresh weight (FW), respectively, and for BS 19 μg g−1 and 0.27 μg g−1 FW. The mRNA levels of 15 carotenogenesis-related genes were analysed during fruit development and ripening. After the breaker stage (S4), the mRNA levels of phytoene synthase 1 (PSY1) and chromoplast-specific lycopene β-cyclase (CYCB) were higher in the peel, and CYCB and β-carotene hydroxylase (BCH) mRNAs were higher in the flesh of LYQ, compared with BS. Plastid morphogenesis during fruit ripening was also studied. The ultrastructure of plastids in the peel of BS changed less than in LYQ during fruit development. Two different chromoplast shapes were observed in the cells of LYQ peel and flesh at the fully ripe stage. Carotenoids were incorporated in the globules in chromoplasts of LYQ and BS peel but were in a crystalline form in the chromoplasts of LYQ flesh. However, no chromoplast structure was found in the cells of fully ripe BS fruit flesh. The mRNA level of plastid lipid-associated protein (PAP) in the peel and flesh of LYQ was over five times higher than in BS peel and flesh. In conclusion, the lower carotenoid content in BS fruit was associated with the lower mRNA levels of PSY1, CYCB, and BCH; however, the failure to develop normal chromoplasts in BS flesh is the most convincing explanation for the lack of carotenoid accumulation. The expression of PAP was well correlated with chromoplast numbers and carotenoid accumulation, suggesting its possible role in chromoplast biogenesis or interconversion of loquat fruit. PMID:21994170

  13. Salinity Stress Is Beneficial to the Accumulation of Chlorogenic Acids in Honeysuckle (Lonicera japonica Thunb.).

    Science.gov (United States)

    Yan, Kun; Cui, Mingxing; Zhao, Shijie; Chen, Xiaobing; Tang, Xiaoli

    2016-01-01

    Honeysuckle ( Lonicera japonica Thunb.) is a traditional medicinal plant in China that is particularly rich in chlorogenic acids, which are phenolic compounds with various medicinal properties. This study aimed to examine the effects of salinity stress on accumulation of chlorogenic acids in honeysuckle, through hydroponic experiments and field trials, and to examine the mechanisms underlying the effects. NaCl stress stimulated the transcription of genes encoding key enzymes in the synthesis of chlorogenic acids in leaves; accordingly, the concentrations of chlorogenic acids in leaves were significantly increased under NaCl stress, as was antioxidant activity. Specifically, the total concentration of leaf chlorogenic acids was increased by 145.74 and 50.34% after 30 days of 150 and 300 mM NaCl stress, respectively. Similarly, the concentrations of chlorogenic acids were higher in the leaves of plants in saline, compared with non-saline, plots, with increases in total concentrations of chlorogenic acids of 56.05 and 105.29% in October 2014 and 2015, respectively. Despite leaf biomass reduction, absolute amounts of chlorogenic acids per plant and phenylalanine ammonia-lyase (PAL) activity were significantly increased by soil salinity, confirming that the accumulation of chlorogenic acids in leaves was a result of stimulation of their synthesis under salinity stress. Soil salinity also led to elevated chlorogenic acid concentrations in honeysuckle flower buds, with significant increases in total chlorogenic acids concentration of 22.42 and 25.14% in May 2014 and 2015, respectively. Consistent with biomass reduction, the absolute amounts of chlorogenic acid per plant declined in flower buds of plants exposed to elevated soil salinity, with no significant change in PAL activity. Thus, salinity-induced chlorogenic acid accumulation in flower buds depended on an amplification effect of growth reduction. In conclusion, salinity stress improved the medicinal quality of

  14. Salinity Stress is Beneficial to the Accumulation of Chlorogenic Acids in Honeysuckle (Lonicera japonica Thunb.

    Directory of Open Access Journals (Sweden)

    Kun Yan

    2016-10-01

    Full Text Available Abstract Honeysuckle (Lonicera japonica Thunb. is a traditional medicinal plant in China that is particularly rich in chlorogenic acids, which are phenolic compounds with various medicinal properties. This study aimed to examine the effects of salinity stress on accumulation of chlorogenic acids in honeysuckle, through hydroponic experiments and field trials, and to examine the mechanisms underlying the effects. NaCl stress stimulated the transcription of genes encoding key enzymes in the synthesis of chlorogenic acids in leaves; accordingly, the concentrations of chlorogenic acids in leaves were significantly increased under NaCl stress, as was antioxidant activity. Specifically, the total concentration of leaf chlorogenic acids was increased by 145.74% and 50.34% after 30 days of 150 and 300 mM NaCl stress, respectively. Similarly, the concentrations of chlorogenic acids were higher in the leaves of plants in saline, compared with non-saline, plots, with increases in total concentrations of chlorogenic acids of 56.05% and 105.29% in October 2014 and 2015, respectively. Despite leaf biomass reduction, absolute amounts of chlorogenic acids per plant and phenylalanine ammonia-lyase (PAL activity were significantly increased by soil salinity, confirming that the accumulation of chlorogenic acids in leaves was a result of stimulation of their synthesis under salinity stress. Soil salinity also led to elevated chlorogenic acid concentrations in honeysuckle flower buds, with significant increases in total chlorogenic acids concentration of 22.42% and 25.14% in May 2014 and 2015, respectively. Consistent with biomass reduction, the absolute amounts of chlorogenic acid per plant declined in flower buds of plants exposed to elevated soil salinity, with no significant change in PAL activity. Thus, salinity-induced chlorogenic acid accumulation in flower buds depended on an amplification effect of growth reduction. In conclusion, salinity stress improves

  15. Determination of the organochlorine insecticide residues in Lonicera japonica Thunb. By gas chromatography%气相色谱法测定金银花中有机氯杀虫剂残留量

    Institute of Scientific and Technical Information of China (English)

    向增旭; 赵维佳; 高山林

    2005-01-01

    Using gas chromatography (GC), the organochlorine insecticide residues were determined in Lonicera japonica Thunb. flowers. The results showed that the amount of organochlorine insecticide in L. japonica flowers collected from Fengqiu in He' nan Province, Pingyi in Shandong Province was less than 0.01 mg·kg-1. The average recoveries added in samples were 93.54% -98.85%, RSD≤6.8%. It shows that the amount of organochlorine insecticide in L. japonica flowers accords with the demands of traditional Chinese medicine production.

  16. Peran Tradisi Lisan Iko-Iko Berbasis Sastra Melayu dalam Penguatan Komunitas Etnis Bajo

    Directory of Open Access Journals (Sweden)

    I Ketut Suardika

    2016-12-01

    Full Text Available Etnia Bajo merupakan  suatu komunitas berbudaya  Melayu hidup secara berkelompok  di berbagai wilayah pesisir pantai dan pulau terpencil eli Nusantara dan Asia Tenggara. Di Kawasan Barat Indonesia dan Malay­ sia Barat disebut Orang Laut, atau Suku Laut. Di Malaysia Timur, Brunai Darussalam, dan Philipina disebut Orang Bajau.Meskipun memiliki nama yang berbeda-beda berdasarkan  geografis tempat tinggalnya, tetapi dari segi  budaya  memiliki  persamaan  khususnya  proses  pewarisan  pengetahuan,  nilai, dan  keterampilan dalam bentuk  penguatan  komunitas  melalui tradisi lisan Iko-iko, nauya (nyanyian dan pantun. Beberapa kajian  menunjukkan   bahwa  Etnis  Bajo  ini  berasal  dari  Selat  Malaka,  selanjutnya   mereka  tersebar  di kawasan  Kepulauan  Melayu  (Malaysia,  Indonesia,  Brunai Darussalam, dan Philipina  akibat  kedatangan imperialisme  Portugis  yang merebut  Malaka  pada tahun 1511, Orang Bajo  sebagai salah satu inti rakyat Kerajaan Malaka bangkit melawan Imperialisme Portugis, setelah kerajaannya  takluk. mereka tetap melan­ jutkan perlawanan  eli laut dengan tersebar  di eli berbagai  kawasan tersebut. Pola pemukiman  yang semi­ nomaden  sebagai  nelayan  tradisional,  mengakibatkan  mereka mengembangkan sistem  pembelajaran  asli (learning comunitas system. Salah satu media pembelajaran  yang banyak digunakan adalah iko-iko (cerita kepahlawanan, jenis  tradisi  lisan  ini terancam  punah karena  kurang  eliminati generasi  muda, umumnya mereka yang bisa mengisahkannya berusia sekitar 50 tahun ke atas. Tradisi sastra iko-iko berperan menyam­ paikan pesan  moral dan semangat  juang yang dituturkan  secara lisan  dari generasi  ke generasi, berkisah tentang kepahlawanan dan elibawakan selama beberapa jam yang dinyanyikan menjelang tidur dan atau saat dalam  pelayaran.  Ciri  khas  iko-iko  adalah  lirik  dan baitnya  secara  teratur  yang

  17. Enzyme expression in indica and japonica rice cultivars under saline stress - doi: 10.4025/actascibiolsci.v34i4.8535

    Directory of Open Access Journals (Sweden)

    Luciano do Amarante

    2012-09-01

    Full Text Available The southern State of Rio Grande do Sul (RS is the main rice producer in Brazil with a 60% participation of the national production and 86% participation of the region. Rice culture irrigation system is done by flooding, which leads to soil salinization, a major environmental constraint to production since it alters the plants’ metabolism exposed to this type of stress. The indica cultivar, widely used in RS, has a higher sensitivity to salinity when compared to that of the japonica cultivar in other physiological aspects. Current research analyzes enzymes expression involved in salt-subjected indica and japonica rice cultivars’ respiration. Oryza sativa L. spp. japonica S.Kato (BRS Bojuru, IAS 12-9 Formosa and Goyakuman and Oryza sativa L. spp. indica S. Kato (BRS Taim-7, BRS Atalanta and BRS Querencia were the cultivars employed. Seedlings were transferred to 15 L basins containing 50% Hoagland nutrient solution increased by 0, 25, 50, 75 and 100 mM NaCl, and collected at 14, 28 and 42 days after transfer (DAT. Plant tissues were macerated and placed in eppendorf tubes with Scandálios extractor solution. Electrophoresis was performed in 7% of the polyacrylamide gels in vertical vats. Bands were revealed for the following enzymes systems: esterase, alcohol dehydrogenase, phosphoglucoisomerase, malate dehydrogenase, malic enzyme and alpha amylase. The enzymes expression was greater in subspecies japonica, with more intense bands in proportion to salinity increase. Results show that enzyme systems are involved in the salinity defense mechanisms in O. sativa spp. japonica cultivar.  

  18. Habilidades pre-escolares de los niños nacidos prematuramente y con bajo peso

    Directory of Open Access Journals (Sweden)

    Sasha A. Martínez-Espiet

    2016-06-01

    Full Text Available RESUMEN: El objetivo de este estudio fue conocer el nivel de desarrollo en la etapa pre-escolar de un grupo de niños nacidos prematuramente y con bajo peso. Se evaluó un grupo 20 niños de cuatro años nacidos prematuramente y con bajo peso y un grupo de 20 niños nacidos luego de gestación completada y peso deseado utilizando la prueba de integración visual-motora Beery-Buktenica. Además, se administró el cuestionario del desarrollo Ages and Stages (ASQ-3 a las 40 madres. El análisis estadístico se llevó a cabo mediante una prueba t de studentpara grupos independientes. El grupo de niños nacidos prematuramente obtuvo puntuaciones significativamente inferiores en las pruebas que medían las habilidades de percepción visual (µ1 83.65 ; µ2 93.7 (p=0.0001, integración visual-motora (µ1 93.6 ; µ2 104.8 (p=0.001 y motor fina (µ1 36.00 ; µ2 44.25 (p=0.033 al ser comparados con el grupo de niños nacidos luego de una gestacióncompletada.El estudio sugiere que los niños nacidos prematuramente y con bajo peso presentan menor ejecución en el desarrollo sensorio-motor durante la etapa pre-escolar. Estas desventajas pasan desapercibidas y pueden representar rezago en las tareas escolares futuras que requieren de dichas habilidades. Es importante fomentar la evaluación y estimulación ambiental temprana en esta población aún en ausencia de indicadores de riesgo. ABSTRACT: The purpose of this study was to evaluate the development among a group of pre-school children born premature and with low weight. We evaluated a group of four years old children; 20 children born prematurely and 20 children born after a full gestation and desired weight, using the Beery-Buktenica visual-motor integration test. We also administered the Ages and Stages Questionnaire (ASQ-3 development test to all 40 mothers. Statistical analysis was performed using student t test for independent groups. The group of children born prematurely scored significantly lower

  19. Discovery and identification of a male-killing agent in the Japanese ladybird Propylea japonica (Coleoptera: Coccinellidae

    Directory of Open Access Journals (Sweden)

    Majerus Michael EN

    2010-02-01

    Full Text Available Abstract Background Endosymbionts that manipulate the reproduction of their hosts have been reported widely in invertebrates. One such group of endosymbionts is the male-killers. To date all male-killers reported are bacterial in nature, but comprise a diverse group. Ladybirds have been described as a model system for the study of male-killing, which has been reported in multiple species from widespread geographic locations. Whilst criteria of low egg hatch-rate and female-biased progenic sex ratio have been used to identify female hosts of male-killers, variation in vertical transmission efficiency and host genetic factors may result in variation in these phenotypic indicators of male-killer presence. Molecular identification of bacteria and screening for bacterial presence provide us with a more accurate method than breeding data alone to link the presence of the bacteria to the male-killing phenotype. In addition, by identifying the bacteria responsible we may find evidence for horizontal transfer between endosymbiont hosts and can gain insight into the evolutionary origins of male-killing. Phylogenetic placement of male-killing bacteria will allow us to address the question of whether male-killing is a potential strategy for only some, or all, maternally inherited bacteria. Together, phenotypic and molecular characterisation of male-killers will allow a deeper insight into the interactions between host and endosymbiont, which ultimately may lead to an understanding of how male-killers identify and kill male-hosts. Results A male-killer was detected in the Japanese coccinellid, Propylea japonica (Thunberg a species not previously known to harbour male-killers. Families produced by female P. japonica showed significantly female-biased sex ratios. One female produced only daughters. This male-killer trait was maternally inherited and antibiotic treatment produced a full, heritable cure. Molecular analysis identified Rickettsia to be associated

  20. QTL Mapping of Grain Quality Traits Using Introgression Lines Carrying Oryza rufipogon Chromosome Segments in Japonica Rice.

    Science.gov (United States)

    Yun, Yeo-Tae; Chung, Chong-Tae; Lee, Young-Ju; Na, Han-Jung; Lee, Jae-Chul; Lee, Sun-Gye; Lee, Kwang-Won; Yoon, Young-Hwan; Kang, Ju-Won; Lee, Hyun-Sook; Lee, Jong-Yeol; Ahn, Sang-Nag

    2016-12-01

    Improved eating quality is a major breeding target in japonica rice due to market demand. Consequently, quantitative trait loci (QTL) for glossiness of cooked rice and amylose content associated with eating quality have received much research focus because of their importance in rice quality. In this study, QTL associated with 12 grain quality traits were identified using 96 introgression lines (IL) of rice developed from an interspecific cross between the Korean elite O. sativa japonica cultivar 'Hwaseong' and O. rufipogon over 7 years. QTL analyses indicated that QTL qDTH6 for heading date, detected on chromosome 6 is associated with variance in grain traits. Most QTLs detected in this study clustered near the qDTH6 locus on chromosome 6, suggesting the effect of qDTH6. O. rufipogon alleles negatively affected grain quality traits except for a few QTLs, including qGCR9 for glossiness of cooked rice on chromosome 9. To characterize the effect of the O. rufipogon locus harboring qGCR9, four lines with a single but different O. rufipogon segment near qGCR9 were compared to Hwaseong. Three lines (O. rufipopgon ILs) having O. rufipogon segment between RM242 and RM245 in common showed higher glossiness of cooked rice than Hwaseong and the other line (Hwaseong IL), indicating that qGCR9 is located in the 3.4-Mb region between RM242 and RM245. Higher glossiness of cooked rice conferred by the O. rufipogon allele might be associated with protein content considering that three lines had lower protein content than Hwaseong (P < 0.1). These three O. rufipogon ILs showed higher yield than Hwaseong and Hwaseong IL due to increase in spikelets per panicle and grain weight indicating the linkage of qGCR9 and yield component QTLs. The qGCR9 locus is of particular interest because of its independence from other undesirable grain quality traits in O. rufipogon. SSR markers linked to qGCR9 can be used to develop high-quality japonica lines and offer a starting point for map

  1. Bajo peso al nacer, crecimiento y desarrollo en el primer año de vida

    Directory of Open Access Journals (Sweden)

    Elia Rosa Lemus Lago

    1997-04-01

    Full Text Available Se efectuó un estudio descriptivo, longitudinal y retrospectivo en el área de salud del Policlínico Docente "19 de Abril" con los niños nacidos con peso inferior a los 2 500 g, en el año 1994, y se siguió su evolución clínica durante el primer año de vida, que correspondió a una muestra de 23 infantes. Los datos fueron recogidos de las historias clínicas individuales de los niños y de la embarazada. Se analizaron los parámetros peso, talla, circunsferencia cefálica, valoración nutricional y desarrollo psicomotor. Predominó el bajo peso para la edad gestacional con un 65,2 y el 34,7 % correspondió a los nacidos antes del término de la gestación. El 100 % de los pretérmino al año de vida tuvieron una valoración nutricional normal. No se comportaron de igual forma los de bajo peso para la edad gestacional en los que el 20 % quedaron por debajo del 3er. percentil.A descriptive, longitudinal and retrospective study was conducted at the health area of the "19 de Abril" Teaching Polyclinic. 23 infants who were born in 1994 with a birth weight < 2 500 g were included in the sample. Their clinical evolution was followed during the first year of life. The data were obtained from the individual medical histories of the children and of the pregnant women. The following parameters were analyzed: weight, size, cephalic perimeter, nutritional assessment and psychomotor development. Low weight prevailed for the gestational age with 65.2 %, whereas 34.7 % corresponded to preterm deliveries. 100 % of the preterm infants had a normal nutritional assessment after the first year of life. However, 20 % of those with low weight at the gestational age remained under the third percentile.

  2. Peso bajo, sobrepeso, obesidad y crecimiento en adolescentes en el Perú 2009-2010

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    Jaime Pajuelo R

    2015-04-01

    Full Text Available Introducción: El grupo de adolescentes representan, de alguna manera, la quinta parte de la población y no se encuentran al margen de los problemas nutricionales. Objetivos: Conocer la situación nutricional, el crecimiento y algunos factores determinantes, en adolescentes en el Perú. Diseño: Estudio descriptivo, observacional, transversal. Participantes: Adolescentes. Intervenciones: En 14 753 adolescentes de 10 a 19 años se obtuvo el peso, talla, índice de masa corporal (IMC. Se utilizó la referencia percentilar de Must y los puntajes Z de OMS. Principales medidas de resultados: Promedios y DE. Prevalencias IC 95%. OR IC 95%. Chi-cuadrado y regresión múltiple. Resultados: Se encontró prevalencias de 2,6; 5,9; 79; 9,3 y 3,2 (Must; 0,2; 1,1; 82,6; 12,5; 3,5% (OMS de déficit, peso bajo, normal, sobrepeso y obesidad, respectivamente. Predominó el sobrepeso-obesidad en áreas urbanas, en los no pobres, en la costa, selva y Lima Metropolitana, en los que vivían por debajo de 3 000 msnm. Los factores de riesgo para el déficit-bajo peso fueron: género masculino, pobres extremos, pobres no extremos, vivir en la costa norte y sierra centro, y como factor protector los que estaban por debajo de los 1 000 msnm. Los factores de riesgo del sobrepeso-obesidad fueron el vivir en áreas urbanas, costa sur y por debajo de los 1 000 msnm, los que vivían entre los 1 000 y 2 999 msnm; y los factores de protección fueron el ser pobre extremo, el pobre no extremo, vivir en costa norte, sierra norte, sierra centro y sierra sur y selva. El 28,5% presentó retardo de crecimiento (adolescentes de 10 a 17 años. Los factores de riesgo del retardo de crecimiento fueron: vivir en áreas rurales, en la mayoría de los dominios geográficos excepto la costa sur, los niveles de pobreza (extrema y no extrema, los niveles de altitud (1 000 a 2 999 y más de 3 000 msnm. Conclusiones: El sobrepeso-obesidad es el problema de mayor magnitud en los adolescentes

  3. lnteracciones medicamentosas en pacientes bajo tratamiento con itraconazol para diferentes tipos de micosis

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    Adriana L. Fernández

    1999-12-01

    Full Text Available Algunos estudios han reportado un aumento en la frecuencia e importancia de las interacciones del itraconazol en pacientes bajo polifarmacia con fármacos que afectan el sistema enzimático de la citocromo P450 y modifican el metabolismo del azol. Con el propósito de determinar las características epidemiológicas, farmacológicas y clínicas de las interacciones medicamentosas en pacientes bajo tratamiento con itraconazol para diferentes tipos de micosis, se realizó un estudio descriptivo transversal retrospectivo en el que se analizaron 21 historias clínicas de pacientes atendidos en la Corporación para Investigaciones Biológicas durante el período 1994-1997. Entre la población estudiada, seis pacientes presentaron paracoccidioidomicosis; cinco, cromoblastomicosis; cuatro, histoplasmosis; cuatro, criptococosis, y dos, aspergilosis. En promedio, la dosis de itraconazol, la duración y el número de fármacos recibidos concomitantes fueron 223,8 mgldia, 44,3 semanas y 2,7 fármacos, respectivamente. En 10 individuos, se presentaron enfermedades de base y en 15, enfermedades concomitantes. Se documentó interacción medicamentosa (niveles no detectables o no terapéuticos de itraconazol en 18 pacientes, de los cuales 17 presentaban sospecha clínica y 14 sospecha paraclínica de interacción. El medicamento más frecuentemente involucrado con interacción fue la rifampicina, seguido de los inhibidores H, antiácidos y fenitoina. Similar a lo encontrado en otros estudios, la administración concomitante de estos fármacos con el itraconazol, reduce los niveles séricos de este último y altera la respuesta clínica al tratamiento. Este estudio es una aproximación inicial, aún no realizada en nuestra población, que permitirá establecer protocolos para el manejo y prevención de interacciones medicamentosas en pacientes en tratamiento con itraconazol y otros fármacos.

  4. Secuelas del neurodesarrollo de recién nacidos prematuros de extremadamente bajo peso y de muy bajo peso a los dos años de edad, egresados de la Unidad de Cuidados Intensivos Neonatales del Hospital Nacional Edgardo Rebagliati Martins 2009-2014

    OpenAIRE

    Fernández Sierra, Carmen; Matzumura Kasano, Juan; Gutiérrez Crespo, Hugo; Zamudio Eslava, Luisa; Melgarejo García, Giannina

    2017-01-01

    Objetivo: Describir las secuelas del neurodesarrollo de los recién nacidos prematuros de extremadamente bajo peso y de muy bajo peso a los dos años de edad, egresados de la Unidad de Cuidados Intensivos Neonatales del Hospital Nacional Edgardo Rebagliati Martins. Materiales y métodos: Estudio descriptivo, retrospectivo, transversal en una población de 190 prematuros de extremadamente bajo peso y de muy bajo peso que nacieron durante enero 2009 a junio del 2014, egresados de la Unidad de Cuida...

  5. The experimental studies of influence of hydrogen sulfide on species of eelgrass (Zostera japonica and Zostera marina) in Padilla Bay, coastal waters of southeast Alaska conducted from 2013-06-01 to 2013-09-30 (NCEI Accession 0137907)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Two species of eelgrass can be found in Padilla Bay, Washington (Zostera japonica and Zostera marina) and act as a bioindicators of ecosystem health. Many factors...

  6. Palmito de pejibaye (Bactris gasipaes cultivado bajo diferentes densidades de laurel (Cordia alliodora

    Directory of Open Access Journals (Sweden)

    Johnny Montenegro

    2001-01-01

    Full Text Available Se evalu6 el incremento en altura y gro- SOT de rebrotes, la altura del tallo dominante, la alliodoproducci6n de palmito y el numero de rebrotes diapOT cepa en plantaciones sin y bajo 2 poblacio- domines de laurel (92 y 370 arboles/ha y el efecto ofdel sombreamiento, en la zona Atlantica de Costa Rica. Se observ6 un mayor incremento en el diametro y la altura de los rebrotes en el monocultivo (P=0.0403, P=0.0033 respectiva- mente unicamente en los estadios iniciales de crecimiento. Una mayor altura del tallo princi- pal (P=0.0143se observ6 en la asociaci6n con haraltas densidades de arboles de laurel. EI nume- ro de rebrotes pOT cepa fue mayor (P=0.05 en maxilas parcelas sin la presencia de arboles. Las ma- yores producciones de palmito fueron encon- whetradas en el monocultivo y con 92 arboles ha/(P=O.O 117, determinandose un menor ren- dimiento con mayores niveles de intercepci6n de luz fotosinteticamente activa pOT parte del estrato arb6reo (r=0.68, P=0.0415. Los ingre- monosos fueron mayores en el tratamiento sin arbo- les, los cuales ejercieron un efecto negativo so- bre el rendimiento productivo del cultivo.

  7. COMPETENCIAS PERSONALES Y PROFESIONALES APLICADAS POR GERENTES BAJO ESCENARIOS DE INCERTIDUMBRE ECONÓMICA

    Directory of Open Access Journals (Sweden)

    Jenniz La Madriz

    2016-04-01

    Full Text Available En Venezuela, las compañías de seguro están operando en un entorno económico de incertidumbre, con reducción de ingresos económicos, inflación e inestabilidad financiera. Ante este escenario, la investigación tuvo como objetivo identificar las competencias que aplican los gerentes del área de seguros, para mantener el negocio posicionado en el mercado, tomando como muestra 22 gerentes. El tipo de investigación es de campo, sustentado en los diseños mixtos secuenciales Creswell (2003. Los resultados permitieron identificar que 47,4% de los gerentes encuestados, manifestaron que de las competencias personales que ponen en práctica en su lugar de trabajo, en contextos de incertidumbre económica, están la responsabilidad - comunicación efectiva - resolución de problemas - negociación y trabajar como parte de un grupo. En cuanto a las competencias profesionales, el 52,6% respondieron que está dedicada a gestionar personal, administrar recursos, planificar - organizar estrategias y rendir resultados, bajo escenarios de incertidumbre económica.

  8. Prospectiva de la cadena Productiva de mango bajo el enfoque dinámico

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    Juan Pablo Tribín-Rivera

    2014-01-01

    Full Text Available El propósito del presente documento de investigación, es relacionar los resultados del análisis prospectivo de la cadena productiva de mango en la Provincia del Tequendama – Cundinamarca por medio de dinámica de sistemas, con el fin de compararla desde distintos escenarios; mercado interno frente al mercado externo, y así poder determinar el escenario prospectivo más conveniente para los productores. De igual forma se proponen políticas que mejoren las condiciones de los productores de la región y se relaciona el modelo de simulación por dinámica de sistemas, que puede ser utilizado en diferentes cadenas productivas. La construcción del modelo de simulación se desarrolló con base en las experiencias adquiridas por medio de visitas de campo, recolección de información primaria y secundaria, y la colaboración de expertos que participan, directa o indirectamente, en dicha cadena productiva, enmarcadas bajo el proyecto de investigación titulado “Mercado prospectivo del mango: Comparativo entre el mercado interno y el mercado externo para la Provincia del Tequendama – Cundinamarca”

  9. La gestion bajo una cultura de calidad en Mexico: una verificacion empirica.

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    Rositas, J.

    2004-07-01

    Full Text Available El artículo presenta de entrada, enfoques administrativos que pueden ser englobados en el término “Gestión bajo una cultura de calidad” (GBCC, conocida internacionalmente como Total Quality Management [TQM], o simplemente Gestión de Calidad, citándose en seguida dos puntos de vista contrapunteados a nivel internacional en relación a este enfoque. Se presentan esfuerzos previos de consultores e implantadores mexicanos que, en base al método de casos y de generalizaciones empíricas de sus experiencias profesionales, consideran a la GBCC como una de las grandes tendencias y fuerzas de cambio en el siglo XXI. Se hace referencia a la escasez de investigaciones en las que se ponga a prueba estadísticamente los conceptos de teóricos, practicantes y consultores del área de la calidad. El autor propone y pone a prueba un modelo agregado de ecuaciones estructurales basado en datos de campo de su investigación de doctorado aportados por 45 empresas manufactureras mexicanas. El análisis de datos, realizado con los paquetes de software SPPS y AMOS, arroja coeficientes de regresión y de determinación, así como niveles de bondad del ajuste, altamente significativos. Al concluir, el autor sugiere estrategias adicionales para refinar futuros análisis de datos.

  10. Robot educativo de bajo costo que interpreta melodías en piano

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    Yeison Alberto Buitrago Suescún

    2013-01-01

    Full Text Available Presenta el diseño de un robot educacional que interpreta melodías en un piano. El propósito principal de este robot es servir como instrumento de prácticas en materias relacionadas con fundamentos de robótica, especialmente en los temas de análisis cinemático. Su control se implementó en el software Matlab, que es de uso común en las universidades. Los materiales y equipos utilizados para su implementación son de fáciladquisición y bajo costo, con el fin de que pueda ser replicado fácilmente por un estudiante. La aplicación del intérprete de melodías en un piano estimula a los estudiantes desde edades tempranas a incursionar en esta área tan importante.

  11. Afrontamiento, salud mental y felicidad bajo huelga y ocupación universitaria

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    Emilio Moyano-Díaz

    2017-01-01

    Full Text Available Se identificó la relación entre estrategias de enfrentamiento, salud mental (SM y felicidad en trabajadores de una universidad en huelga y ocupación estudiantil, de acuerdo con status laboral - académico o administrativo-, grado de concordancia con el movimiento estudiantil y sexo. Ciento sesenta y dos participantes respondieron a la Escala de felicidad subjetiva y estrategias de enfrentamiento, y a la Escala de Goldberg (GHQ12. Obtuvimos y discutimos: 1 el uso del humor está asociado con SM y la felicidad y ésta, a la reevaluación cognitiva, enfrentamiento religioso y bajo enfrentamiento de evitación, 2 mayor malestar por la ocupación peor SM, 3 no tener acceso a el espacio de trabajo está asociado con peor SM, más con iguales niveles de felicidad que los que tuvieron acceso, 4 el enfrentamiento varía de acuerdo con el status de académico o administrativo y con el sexo, 5 SM y felicidad no varían de acuerdo con el status académico, 6 aquellos que son pro-movimiento estudiantil presentan mejor SM que aquellos que no lo son.

  12. Modelo matemático de un sistema coordinado productor-comprador bajo el enfoque VMI

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    Fidel Torres

    2012-12-01

    Full Text Available En este trabajo se desarrolla un nuevo modelo matemático de un sistema coordinado productor-comprador mediante un acuerdo de inventarios administrados por el vendedor (VMI. El modelo propuesto considera el tiempo de fabricación del lote e incluye un esquema de sincronización entre el productor y el comprador que facilita la coordinación de la cadena de suministros. El análisis del modelo matemático de coordinación tiene en cuenta las tasas de producción y de demanda y los costos totales anuales de ordenar y mantener inventarios del productor y del comprador. El estudio es complementado por un análisis de sensibilidad que se enfoca sobre los efectos de las variaciones de los parámetros sobre algunas medidas de desempeño en el esquema coordinado VMI entre el productor y el comprador.  Finalmente, se deducen y verifican las condiciones analíticas bajo las cuales la implementación coordinada de VMI otorga beneficios al productor, al comprador y a la cadena de suministros. Los resultados muestran que es posible que el productor y el comprador obtengan beneficios en la implementación de VMI seleccionando una combinación apropiada de los parámetros usando el esquema de coordinación propuesto en una relación gana-gana.

  13. Evaluación de un cluster bajo agricultura protegida en México

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    Luz Evelia Padilla-Bernal

    2012-01-01

    Full Text Available El naciente cluster de tomate bajo agricultura protegida en Zacatecas enfrenta el reto de participar en los mercados globales de una manera sostenible; una respuesta viable para ello es elevar su capacidad de upgrading. En este trabajo se determina la relación entre la eficiencia colectiva en el upgrading del cluster. La información se obtuvo a través de un cuestionario aplicado a técnicos de las unidades de producción (UP, el cual se complementó con entrevistas a propietarios o gerentes, investigadores y funcionarios gubernamentales; posteriormente, se hizo una clasificación de las UP por nivel tecnológico aplicando la técnica de análisis de conglomerado con lo que se obtuvo el índice de eficiencia colectiva, el tipo de gobernanza de las cadenas de valor con las que se relacionan las agroempresas y los tipos de upgrading logrados. Se encontró que las economías externas fueron más dominantes que las acciones conjuntas; la eficiencia colectiva y el upgrading se encuentran positivamente relacionados; asimismo, se observó que el alto nivel tecnológico es condición necesaria, más no suficiente, para que las agroempresas permanezcan competitivas en el mercado global, por lo que se requiere elevar su capacidad de upgrading.

  14. Selective LXRα inhibitory effects observed in plant extracts of MEH184 (Parthenocissua tricuspidata) and MEH185 (Euscaphis japonica)

    International Nuclear Information System (INIS)

    Kim, Kang Ho; Choi, Seung Hyun; Lee, Thomas S.; Oh, Won Keun; Kim, Dong Sun; Kim, Jae Bum

    2006-01-01

    Liver X receptors (LXRs) are nuclear hormone receptors that behave as lipid sensors of cellular cholesterol and fatty acid. Although LXR activation can alleviate hypercholesterolemia by inducing cholesterol efflux, it also results in undesirable effects of fatty acid synthesis, resulting in hepatic steatosis and hyperlipidemia. Therefore, it is critical to identify LXRα inhibitory agents that would repress fatty acid synthesis and hepatic lipid accumulation. In current study, screening of plant extracts used for traditional oriental medicine resulted in the identification of two candidates demonstrating selective LXRα inhibitory activity. These were whole leaf methanol extracts of Parthenocissua tricuspidata (MEH184) and Euscaphis japonica (MEH185). Both MEH184 and MEH185 decreased transcriptional activity of LXRα and the expression of LXRα target genes, such as FAS and ADD1/SREBP1c. Additionally, MEH184 and MEH184 significantly reduced lipogenesis and adipocyte differentiation. Together, the data imply that MEH184 and MEH185 possess selective antagonistic properties on LXRα to downregulate lipogenesis

  15. Detection of bacterial contaminants and hybrid sequences in the genome of the kelp Saccharina japonica using Taxoblast

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    Simon M. Dittami

    2017-11-01

    Full Text Available Modern genome sequencing strategies are highly sensitive to contamination making the detection of foreign DNA sequences an important part of analysis pipelines. Here we use Taxoblast, a simple pipeline with a graphical user interface, for the post-assembly detection of contaminating sequences in the published genome of the kelp Saccharina japonica. Analyses were based on multiple blastn searches with short sequence fragments. They revealed a number of probable bacterial contaminations as well as hybrid scaffolds that contain both bacterial and algal sequences. This or similar types of analysis, in combination with manual curation, may thus constitute a useful complement to standard bioinformatics analyses prior to submission of genomic data to public repositories. Our analysis pipeline is open-source and freely available at http://sdittami.altervista.org/taxoblast and via SourceForge (https://sourceforge.net/projects/taxoblast.

  16. Comparison of learning ability and memory retention in altricial (Bengalese finch, Lonchura striata var. domestica) and precocial (blue-breasted quail, Coturnix chinensis) birds using a color discrimination task.

    Science.gov (United States)

    Ueno, Aki; Suzuki, Kaoru

    2014-02-01

    The present study sought to assess the potential application of avian models with different developmental modes to studies on cognition and neuroscience. Six altricial Bengalese finches (Lonchura striata var. domestica), and eight precocial blue-breasted quails (Coturnix chinensis) were presented with color discrimination tasks to compare their respective faculties for learning and memory retention within the context of the two developmental modes. Tasks consisted of presenting birds with discriminative cues in the form of colored feeder lids, and birds were considered to have learned a task when 80% of their attempts at selecting the correctly colored lid in two consecutive blocks of 10 trials were successful. All of the finches successfully performed the required experimental tasks, whereas only half of the quails were able to execute the same tasks. In the learning test, finches required significantly fewer trials than quails to learn the task (finches: 13.5 ± 9.14 trials, quails: 45.8 ± 4.35 trials, P memory retention tests, which were conducted 45 days after the learning test, finches retained the ability to discriminate between colors correctly (95.0 ± 4.47%), whereas quails did not retain any memory of the experimental procedure and so could not be tested. These results suggested that altricial and precocial birds both possess the faculty for learning and retaining discrimination-type tasks, but that altricial birds perform better than precocial birds in both faculties. The present findings imply that developmental mode is an important consideration for assessing the suitability of bird species for particular experiments. © 2013 Japanese Society of Animal Science.

  17. Determination of male strobilus developmental stages by cytological and gene expression analyses in Japanese cedar (Cryptomeria japonica).

    Science.gov (United States)

    Tsubomura, Miyoko; Kurita, Manabu; Watanabe, Atsushi

    2016-05-01

    The molecular mechanisms that control male strobilus development in conifers are largely unknown because the developmental stages and related genes have not yet been characterized. The determination of male strobilus developmental stages will contribute to genetic research and reproductive biology in conifers. Our objectives in this study were to determine the developmental stages of male strobili by cytological and transcriptome analysis, and to determine the stages at which aberrant morphology is observed in a male-sterile mutant of Cryptomeria japonica D. Don to better understand the molecular mechanisms that control male strobilus and pollen development. Male strobilus development was observed for 8 months, from initiation to pollen dispersal. A set of 19,209 expressed sequence tags (ESTs) collected from a male reproductive library and a pollen library was used for microarray analysis. We divided male strobilus development into 10 stages by cytological and transcriptome analysis. Eight clusters (7324 ESTs) exhibited major changes in transcriptome profiles during male strobili and pollen development in C. japonica Two clusters showed a gradual increase and decline in transcript abundance, respectively, while the other six clusters exhibited stage-specific changes. The stages at which the male sterility trait of Sosyun was expressed were identified using information on male strobilus and pollen developmental stages and gene expression profiles. Aberrant morphology was observed cytologically at Stage 6 (microspore stage), and differences in expression patterns compared with wild type were observed at Stage 4 (tetrad stage). © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  18. Effects of soil water content and elevated CO2 concentration on the monoterpene emission rate of Cryptomeria japonica.

    Science.gov (United States)

    Mochizuki, Tomoki; Amagai, Takashi; Tani, Akira

    2018-04-11

    Monoterpenes emitted from plants contribute to the formation of secondary pollution and affect the climate system. Monoterpene emission rates may be affected by environmental changes such as increasing CO 2 concentration caused by fossil fuel burning and drought stress induced by climate change. We measured monoterpene emissions from Cryptomeria japonica clone saplings grown under different CO 2 concentrations (control: ambient CO 2 level, elevated CO 2 : 1000μmolmol -1 ). The saplings were planted in the ground and we did not artificially control the SWC. The relationship between the monoterpene emissions and naturally varying SWC was investigated. The dominant monoterpene was α-pinene, followed by sabinene. The monoterpene emission rates were exponentially correlated with temperature for all measurements and normalized (35°C) for each measurement day. The daily normalized monoterpene emission rates (E s0.10 ) were positively and linearly correlated with SWC under both control and elevated CO 2 conditions (control: r 2 =0.55, elevated CO 2 : r 2 =0.89). The slope of the regression line of E s0.10 against SWC was significantly higher under elevated CO 2 than under control conditions (ANCOVA: P<0.01), indicating that the effect of CO 2 concentration on monoterpene emission rates differed by soil water status. The monoterpene emission rates estimated by considering temperature and SWC (Improved G93 algorithm) better agreed with the measured monoterpene emission rates, when compared with the emission rates estimated by considering temperature alone (G93 algorithm). Our results demonstrated that the combined effects of SWC and CO 2 concentration are important for controlling the monoterpene emissions from C. japonica clone saplings. If these relationships can be applied to the other coniferous tree species, our results may be useful to improve accuracy of monoterpene emission estimates from the coniferous forests as affected by climate change in the present and

  19. Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress

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    Wijaya Edward

    2010-01-01

    Full Text Available Abstract Background The transcriptional regulatory network involved in low temperature response leading to acclimation has been established in Arabidopsis. In japonica rice, which can only withstand transient exposure to milder cold stress (10°C, an oxidative-mediated network has been proposed to play a key role in configuring early responses and short-term defenses. The components, hierarchical organization and physiological consequences of this network were further dissected by a systems-level approach. Results Regulatory clusters responding directly to oxidative signals were prominent during the initial 6 to 12 hours at 10°C. Early events mirrored a typical oxidative response based on striking similarities of the transcriptome to disease, elicitor and wounding induced processes. Targets of oxidative-mediated mechanisms are likely regulated by several classes of bZIP factors acting on as1/ocs/TGA-like element enriched clusters, ERF factors acting on GCC-box/JAre-like element enriched clusters and R2R3-MYB factors acting on MYB2-like element enriched clusters. Temporal induction of several H2O2-induced bZIP, ERF and MYB genes coincided with the transient H2O2 spikes within the initial 6 to 12 hours. Oxidative-independent responses involve DREB/CBF, RAP2 and RAV1 factors acting on DRE/CRT/rav1-like enriched clusters and bZIP factors acting on ABRE-like enriched clusters. Oxidative-mediated clusters were activated earlier than ABA-mediated clusters. Conclusion Genome-wide, physiological and whole-plant level analyses established a holistic view of chilling stress response mechanism of japonica rice. Early response regulatory network triggered by oxidative signals is critical for prolonged survival under sub-optimal temperature. Integration of stress and developmental responses leads to modulated growth and vigor maintenance contributing to a delay of plastic injuries.

  20. Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica

    Science.gov (United States)

    Yang, Jingwen; Xu, Yuchao; Xu, Ke; Ping, Hongling; Shi, Huilai; Lü, Zhenming; Wu, Changwen; Wang, Tianming

    2017-08-01

    Neuropeptide Y (NPY) has a pivotal role in the regulation of many physiological processes. In this study, the gene encoding a NPY receptor-like from the common Chinese cuttlefish Sepiella japonica (SjNPYR-like) was identified and characterized. The full-length SjNPYR-like cDNA was cloned containing a 492-bp of 5' untranslated region (UTR), 1 182 bp open reading frame (ORF) encoding a protein of 393 amino acid residues, and 228 bp of 3' UTR. The putative protein was predicted to have a molecular weight of 45.54 kDa and an isoelectric point (pI) of 8.13. By informatic analyses, SjNPYR-like was identified as belonging to the class A G protein coupled receptor (GPCR) family (the rhodopsin-type). The amino acid sequence contained 12 potential phosphorylation sites and five predicted N-linked glycosylation sites. Multiple sequence alignment and 3D structure modeling were conducted to clarify SjNPYR bioinformatics characteristics. Phylogenetic analysis identifies it as an NPYR with identity of 33% to Lymnaea stagnalis NPFR. Transmembrane properties of SjNPYR-like were demonstrated in vitro using HEK293 cells and the pEGFP-N1 plasmid. Relative quantification of SjNPYR-like mRNA level confirmed a high level expression and broad distribution of SjNPYR - like in various tissues of female S. japonica. In addition, the transcriptional profile of SjNPYR - like in the brain, liver, and ovary during gonadal development was analyzed. The results provide basic understanding on the molecular characteristics of SjNPYR-like and its potentially physical functions.

  1. Arabinogalactan Proteins Accumulate in the Cell Walls of Searching Hyphae of the Stem Parasitic Plants, Cuscuta campestris and Cuscuta japonica.

    Science.gov (United States)

    Hozumi, Akitaka; Bera, Subhankar; Fujiwara, Daiki; Obayashi, Takeshi; Yokoyama, Ryusuke; Nishitani, Kazuhiko; Aoki, Koh

    2017-11-01

    Stem parasitic plants (Cuscuta spp.) develop a specialized organ called a haustorium to penetrate their hosts' stem tissues. To reach the vascular tissues of the host plant, the haustorium needs to overcome the physical barrier of the cell wall, and the parasite-host interaction via the cell wall is a critical process. However, the cell wall components responsible for the establishment of parasitic connections have not yet been identified. In this study, we investigated the spatial distribution patterns of cell wall components at a parasitic interface using parasite-host complexes of Cuscuta campestris-Arabidopsis thaliana and Cuscuta japonica-Glycine max. We focused on arabinogalactan proteins (AGPs), because AGPs accumulate in the cell walls of searching hyphae of both C. campestris and C. japonica. We found more AGPs in elongated haustoria than in pre haustoria, indicating that AGP accumulation is developmentally regulated. Using in situ hybridization, we identified five genes in C. campestris that encode hyphal-expressed AGPs that belong to the fasciclin-like AGP (FLA) family, which were named CcFLA genes. Three of the five CcFLA genes were expressed in the holdfast, which develops on the Cuscuta stem epidermis at the attachment site for the host's stem epidermis. Our results suggest that AGPs are involved in hyphal elongation and adhesion to host cells, and in the adhesion between the epidermal tissues of Cuscuta and its host. © The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  2. Antiobesity Effects of the Ethanol Extract of Laminaria japonica Areshoung in High-Fat-Diet-Induced Obese Rat

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    Woong Sun Jang

    2013-01-01

    Full Text Available Laminaria japonica Areshoung, a widely consumed marine vegetable, has traditionally been used in Korean maternal health. The present study investigated the antiobesity effects of Laminaria japonica Areshoung ethanol extract (LE and its molecular mechanism in high-fat-diet-induced obese rats. Six-week-old Sprague-Dawley male rats were separately fed a normal diet or a high-calorie high-fat diet for 6 weeks; then they were treated with LE or tea catechin for another 6 weeks. LE administration significantly decreased the body weight gain, fat-pad weights, and serum and hepatic lipid levels in HD-induced obese rats. The histological analysis revealed that LE-treated group showed a significantly decreased number of lipid droplets and size of adipocytes compared to the HD group. To elucidate the mechanism of action of LE, the levels of genes and proteins involved in obesity were measured in the liver and skeletal muscle. LE treatment resulted in an increased expression of fatty acid oxidation and thermogenesis-related genes in obese rats. Conversely, the expression of the fat intake-related gene (ACC2 and lipogenesis-related genes was reduced by LE treatment. Additionally, LE treatment increased the phosphorylation of AMP-activated protein kinase and its direct downstream protein, acetyl coenzyme A carboxylase, which is one of the rate-limiting enzymes in fatty acid synthesis pathway. These findings demonstrate that LE treatment has a protective effect against a high-fat-diet-induced obesity in rats through regulation of expression of genes and proteins involved in lipolysis and lipogenesis.

  3. Evaluation of the agronomic performance of atrazine-tolerant transgenic japonica rice parental lines for utilization in hybrid seed production.

    Directory of Open Access Journals (Sweden)

    Luhua Zhang

    Full Text Available Currently, the purity of hybrid seed is a crucial limiting factor when developing hybrid japonica rice (Oryza sativa L.. To chemically control hybrid seed purity, we transferred an improved atrazine chlorohydrolase gene (atzA from Pseudomonas ADP into hybrid japonica parental lines (two maintainers, one restorer, and Nipponbare, by using Agrobacterium-mediated transformation. We subsequently selected several transgenic lines from each genotype by using PCR, RT-PCR, and germination analysis. In the presence of the investigated atrazine concentrations, particularly 150 µM atrazine, almost all of the transgenic lines produced significantly larger seedlings, with similar or higher germination percentages, than did the respective controls. Although the seedlings of transgenic lines were taller and gained more root biomass compared to the respective control plants, their growth was nevertheless inhibited by atrazine treatment compared to that without treatment. When grown in soil containing 2 mg/kg or 5 mg/kg atrazine, the transgenic lines were taller, and had higher total chlorophyll contents than did the respective controls; moreover, three of the strongest transgenic lines completely recovered after 45 days of growth. After treatment with 2 mg/kg or 5 mg/kg of atrazine, the atrazine residue remaining in the soil was 2.9-7.0% or 0.8-8.7% respectively, for transgenic lines, and 44.0-59.2% or 28.1-30.8%, respectively, for control plants. Spraying plants at the vegetative growth stage with 0.15% atrazine effectively killed control plants, but not transgenic lines. Our results indicate that transgenic atzA rice plants show tolerance to atrazine, and may be used as parental lines in future hybrid seed production.

  4. Camouflage Effects of Various Colour-Marking Morphs against Different Microhabitat Backgrounds in a Polymorphic Pygmy Grasshopper Tetrix japonica

    Science.gov (United States)

    Tsurui, Kaori; Honma, Atsushi; Nishida, Takayoshi

    2010-01-01

    Background Colour-marking polymorphism is widely distributed among cryptic species. To account for the adaptive significance of such polymorphisms, several hypotheses have been proposed to date. Although these hypotheses argue over the degree of camouflage effects of marking morphs (and the interactions between morphs and their microhabitat backgrounds), as far as we know, most empirical evidence has been provided under unnatural conditions (i.e., using artificial prey). Methodology/Principal Findings Tetrix japonica, a pygmy grasshopper, is highly polymorphic in colour-markings and occurs in both sand and grass microhabitats. Even within a microhabitat, T. japonica is highly polymorphic. Using humans as dummy predators and printed photographs in which various morphs of grasshoppers were placed against different backgrounds, we addressed three questions to test the neutral, background heterogeneity, and differential crypsis hypotheses in four marking-type morphs: 1) do the morphs differ in the degree of crypsis in each microhabitat, 2) are different morphs most cryptic in specific backgrounds of the microhabitats, and 3) does the morph frequency reflect the degree of crypsis? Conclusions/Significance The degree of camouflage differed among the four morphs; therefore, the neutral hypothesis was rejected. Furthermore, the order of camouflage advantage among morphs differed depending on the two types of backgrounds (sand and grass), although the grass background consistently provided greater camouflage effects. Thus, based on our results, we could not reject the background heterogeneity hypothesis. Under field conditions, the more cryptic morphs comprised a minority of the population. Overall, our results demonstrate that the different morphs were not equivalent in the degree of crypsis, but the degree of camouflage of the morphs was not consistent with the morph frequency. These findings suggest that trade-offs exist between the camouflage benefit of body colouration

  5. Few effects of invasive plants Reynoutria japonica, Rudbeckia laciniata and Solidago gigantea on soil physical and chemical properties.

    Science.gov (United States)

    Stefanowicz, Anna M; Stanek, Małgorzata; Nobis, Marcin; Zubek, Szymon

    2017-01-01

    Biological invasions are an important problem of human-induced changes at a global scale. Invasive plants can modify soil nutrient pools and element cycling, creating feedbacks that potentially stabilize current or accelerate further invasion, and prevent re-establishment of native species. The aim of this study was to compare the effects of Reynoutria japonica, Rudbeckia laciniata and Solidago gigantea, invading non-forest areas located within or outside river valleys, on soil physical and chemical parameters, including soil moisture, element concentrations, organic matter content and pH. Additionally, invasion effects on plant species number and total plant cover were assessed. The concentrations of elements in shoots and roots of invasive and native plants were also measured. Split-plot ANOVA revealed that the invasions significantly reduced plant species number, but did not affect most soil physical and chemical properties. The invasions decreased total P concentration and increased N-NO 3 concentration in soil in comparison to native vegetation, though the latter only in the case of R. japonica. The influence of invasion on soil properties did not depend on location (within- or outside valleys). The lack of invasion effects on most soil properties does not necessarily imply the lack of influence of invasive plants, but may suggest that the direction of the changes varies among replicate sites and there are no general patterns of invasion-induced alterations for these parameters. Tissue element concentrations, with the exception of Mg, did not differ between invasive and native plants, and were not related to soil element concentrations. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. Development and characterization of japonica rice lines carrying the brown planthopper-resistance genes BPH12 and BPH6.

    Science.gov (United States)

    Qiu, Yongfu; Guo, Jianping; Jing, Shengli; Zhu, Lili; He, Guangcun

    2012-02-01

    The brown planthopper (Nilaparvata lugens Stål; BPH) has become a severe constraint on rice production. Identification and pyramiding BPH-resistance genes is an economical and effective solution to increase the resistance level of rice varieties. All the BPH-resistance genes identified to date have been from indica rice or wild species. The BPH12 gene in the indica rice accession B14 is derived from the wild species Oryza latifolia. Using an F(2) population from a cross between the indica cultivar 93-11 and B14, we mapped the BPH12 gene to a 1.9-cM region on chromosome 4, flanked by the markers RM16459 and RM1305. In this population, BPH12 appeared to be partially dominant and explained 73.8% of the phenotypic variance in BPH resistance. A near-isogenic line (NIL) containing the BPH12 locus in the background of the susceptible japonica variety Nipponbare was developed and crossed with a NIL carrying BPH6 to generate a pyramid line (PYL) with both genes. BPH insects showed significant differences in non-preference in comparisons between the lines harboring resistance genes (NILs and PYL) and Nipponbare. BPH growth and development were inhibited and survival rates were lower on the NIL-BPH12 and NIL-BPH6 plants compared to the recurrent parent Nipponbare. PYL-BPH6 + BPH12 exhibited 46.4, 26.8 and 72.1% reductions in population growth rates (PGR) compared to NIL-BPH12, NIL-BPH6 and Nipponbare, respectively. Furthermore, insect survival rates were the lowest on the PYL-BPH6 + BPH12 plants. These results demonstrated that pyramiding different BPH-resistance genes resulted in stronger antixenotic and antibiotic effects on the BPH insects. This gene pyramiding strategy should be of great benefit for the breeding of BPH-resistant japonica rice varieties.

  7. Ulmus davidiana var. japonica Nakai upregulates eosinophils and suppresses Th1 and Th17 cells in the small intestine.

    Directory of Open Access Journals (Sweden)

    Han-Sung Lee

    Full Text Available The bark of Ulmus davidiana var. japonica Nakai (Ulmaceae has been used in traditional Korean medicine for chronic inflammation in the gastrointestinal tract. Here we investigated the frequency and cytokine profile of the major immune cells in the small intestinal lamina propria (SI LP, spleen, and mesenteric lymph nodes (MLNs of mice treated orally with Ulmus davidiana var. japonica Nakai bark water extract (UDE to address the immunomodulatory role of this herb in intestinal homeostasis. B6 mice were given 5g/kg UDE once daily for 14 days. They were then sacrificed, and cells were isolated from the spleen, MLNs, and SI LP. The proportion of B versus T lymphocytes, CD4(+ versus CD8(+ T lymphocytes, Th1 and Th17 cells, and Foxp3(+ regulatory T cells in the spleen, MLNs, and SI LP were analyzed. The frequency of antigen-presenting cells (APCs, including dendritic cells, macrophages, and eosinophils in the SI LP and the expression of costimulatory molecules on APCs were also evaluated. The numbers and frequencies of Th1 and Th17 cells in the SI LP were significantly reduced in the UDE-treated mice compared with PBS controls. In addition, the proportion of IL-4-producing eosinophils in the SI LP was significantly elevated in the UDE-treated mice compared with controls. Taken together, these data indicate that UDE up-regulates the number and frequency of SI LP eosinophils, which can down-regulate the Th1 and Th17 responses via IL-4 secretion and contribute to intestinal homeostasis.

  8. Transmission Risks of Schistosomiasis Japonica: Extraction from Back-propagation Artificial Neural Network and Logistic Regression Model

    Science.gov (United States)

    Xu, Jun-Fang; Xu, Jing; Li, Shi-Zhu; Jia, Tia-Wu; Huang, Xi-Bao; Zhang, Hua-Ming; Chen, Mei; Yang, Guo-Jing; Gao, Shu-Jing; Wang, Qing-Yun; Zhou, Xiao-Nong

    2013-01-01

    Background The transmission of schistosomiasis japonica in a local setting is still poorly understood in the lake regions of the People's Republic of China (P. R. China), and its transmission patterns are closely related to human, social and economic factors. Methodology/Principal Findings We aimed to apply the integrated approach of artificial neural network (ANN) and logistic regression model in assessment of transmission risks of Schistosoma japonicum with epidemiological data collected from 2339 villagers from 1247 households in six villages of Jiangling County, P.R. China. By using the back-propagation (BP) of the ANN model, 16 factors out of 27 factors were screened, and the top five factors ranked by the absolute value of mean impact value (MIV) were mainly related to human behavior, i.e. integration of water contact history and infection history, family with past infection, history of water contact, infection history, and infection times. The top five factors screened by the logistic regression model were mainly related to the social economics, i.e. village level, economic conditions of family, age group, education level, and infection times. The risk of human infection with S. japonicum is higher in the population who are at age 15 or younger, or with lower education, or with the higher infection rate of the village, or with poor family, and in the population with more than one time to be infected. Conclusion/Significance Both BP artificial neural network and logistic regression model established in a small scale suggested that individual behavior and socioeconomic status are the most important risk factors in the transmission of schistosomiasis japonica. It was reviewed that the young population (≤15) in higher-risk areas was the main target to be intervened for the disease transmission control. PMID:23556015

  9. Chemical Composition and Antioxidant and Antibacterial Activities of an Essential Oil Extracted from an Edible Seaweed, Laminaria japonica L.

    Science.gov (United States)

    Patra, Jayanta Kumar; Das, Gitishree; Baek, Kwang-Hyun

    2015-07-02

    Laminaria japonica L. is among the most commonly consumed seaweeds in northeast Asia. In the present study, L. japonica essential oil (LJEO) was extracted by microwave-hydrodistillation and analyzed by gas chromatography and mass spectroscopy. LJEO contained 21 volatile compounds, comprising 99.76% of the total volume of the essential oil, primarily tetradeconoic acid (51.75%), hexadecanoic acid (16.57%), (9Z,12Z)-9,12-Octadecadienoic acid (12.09%), and (9Z)-hexadec-9-enoic acid (9.25%). Evaluation of the antibacterial potential against three foodborne pathogens, Bacillus cereus ATCC 10876, Escherichia coli O157:H7 ATCC 43890, and Staphylococcus aureus ATCC 49444, revealed that LJEO at a concentration of 25 mg/paper disc exerted high antibacterial activity against S. aureus (11.5 ± 0.58 mm inhibition zone) and B. cereus (10.5 ± 0.57 mm inhibition zone), but no inhibition of E. coli O157:H7. LJEO also displayed DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity (80.45%), superoxide anion scavenging activity (54.03%), and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical and hydroxyl radical scavenging at 500 µg/mL. Finally, LJEO showed high inhibition of lipid peroxidation with strong reducing power. In conclusion, LJEO from edible seaweed is an inexpensive but favorable resource with strong antibacterial capacity as well as free radical scavenging and antioxidant activity; therefore, it has the potential for use in the food, cosmetics, and pharmaceutical industries.

  10. Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress

    KAUST Repository

    Yun, Kil-Young

    2010-01-25

    Background: The transcriptional regulatory network involved in low temperature response leading to acclimation has been established in Arabidopsis. In japonica rice, which can only withstand transient exposure to milder cold stress (10C), an oxidative-mediated network has been proposed to play a key role in configuring early responses and short-term defenses. The components, hierarchical organization and physiological consequences of this network were further dissected by a systems-level approach.Results: Regulatory clusters responding directly to oxidative signals were prominent during the initial 6 to 12 hours at 10C. Early events mirrored a typical oxidative response based on striking similarities of the transcriptome to disease, elicitor and wounding induced processes. Targets of oxidative-mediated mechanisms are likely regulated by several classes of bZIP factors acting on as1/ocs/TGA-like element enriched clusters, ERF factors acting on GCC-box/JAre-like element enriched clusters and R2R3-MYB factors acting on MYB2-like element enriched clusters.Temporal induction of several H2O2-induced bZIP, ERF and MYB genes coincided with the transient H2O2spikes within the initial 6 to 12 hours. Oxidative-independent responses involve DREB/CBF, RAP2 and RAV1 factors acting on DRE/CRT/rav1-like enriched clusters and bZIP factors acting on ABRE-like enriched clusters. Oxidative-mediated clusters were activated earlier than ABA-mediated clusters.Conclusion: Genome-wide, physiological and whole-plant level analyses established a holistic view of chilling stress response mechanism of japonica rice. Early response regulatory network triggered by oxidative signals is critical for prolonged survival under sub-optimal temperature. Integration of stress and developmental responses leads to modulated growth and vigor maintenance contributing to a delay of plastic injuries. 2010 Yun et al; licensee BioMed Central Ltd.

  11. Chemical Composition and Antioxidant and Antibacterial Activities of an Essential Oil Extracted from an Edible Seaweed, Laminaria japonica L.

    Directory of Open Access Journals (Sweden)

    Jayanta Kumar Patra

    2015-07-01

    Full Text Available Laminaria japonica L. is among the most commonly consumed seaweeds in northeast Asia. In the present study, L. japonica essential oil (LJEO was extracted by microwave-hydrodistillation and analyzed by gas chromatography and mass spectroscopy. LJEO contained 21 volatile compounds, comprising 99.76% of the total volume of the essential oil, primarily tetradeconoic acid (51.75%, hexadecanoic acid (16.57%, (9Z,12Z-9,12-Octadecadienoic acid (12.09%, and (9Z-hexadec-9-enoic acid (9.25%. Evaluation of the antibacterial potential against three foodborne pathogens, Bacillus cereus ATCC 10876, Escherichia coli O157:H7 ATCC 43890, and Staphylococcus aureus ATCC 49444, revealed that LJEO at a concentration of 25 mg/paper disc exerted high antibacterial activity against S. aureus (11.5 ± 0.58 mm inhibition zone and B. cereus (10.5 ± 0.57 mm inhibition zone, but no inhibition of E. coli O157:H7. LJEO also displayed DPPH (1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity (80.45%, superoxide anion scavenging activity (54.03%, and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radical and hydroxyl radical scavenging at 500 µg/mL. Finally, LJEO showed high inhibition of lipid peroxidation with strong reducing power. In conclusion, LJEO from edible seaweed is an inexpensive but favorable resource with strong antibacterial capacity as well as free radical scavenging and antioxidant activity; therefore, it has the potential for use in the food, cosmetics, and pharmaceutical industries.

  12. Effects of field high temperature on grain yield and quality of a subtropical type japonica rice—Pon-Lai rice

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    Yi-Chien Wu

    2016-01-01

    Full Text Available Typical japonica type rice is sensitive to high temperature. Pon-Lai rice is a special japonica type with adaptation to the subtropical climate in Taiwan. Facing climate change, rising temperatures would damage the yield and quality of rice production. This research was conducted using Pon-Lai rice in the field of a subtropical climate. We conducted 2 experiments, including a year-round experiment and collection of samples from different districts for building different temperature conditions. We analyzed the correlation between rising temperature and rice yield or quality. In our results, the critical period of temperature effect is 0–15 days after heading (H15. The threshold of high temperature damage in yield and appearance quality was 25–27 °C. Grain weight decreased about 2–6%, while the temperature of H15 was raised 1 °C above the thresholds. Perfect grain ratio and chalky grain ratio decreased and increased, respectively, while the temperature of H15 was raised above the thresholds. However, the high temperature in H15 affected the physicochemical characteristics. In addition, we found positive correlation between grain length to width ratio and perfect grain ratio. Grain length to width ratio could be an index of temperature effects for grain quality. In our study, when the temperature was below 30 °C, a rising temperature of H15 could damage rice yield and appearance quality, and change grain shape. Our results could provide reference for dealing with the warming future in other temperate rice-cultivated countries.

  13. Camouflage effects of various colour-marking morphs against different microhabitat backgrounds in a polymorphic pygmy grasshopper Tetrix japonica.

    Science.gov (United States)

    Tsurui, Kaori; Honma, Atsushi; Nishida, Takayoshi

    2010-07-06

    Colour-marking polymorphism is widely distributed among cryptic species. To account for the adaptive significance of such polymorphisms, several hypotheses have been proposed to date. Although these hypotheses argue over the degree of camouflage effects of marking morphs (and the interactions between morphs and their microhabitat backgrounds), as far as we know, most empirical evidence has been provided under unnatural conditions (i.e., using artificial prey). Tetrix japonica, a pygmy grasshopper, is highly polymorphic in colour-markings and occurs in both sand and grass microhabitats. Even within a microhabitat, T. japonica is highly polymorphic. Using humans as dummy predators and printed photographs in which various morphs of grasshoppers were placed against different backgrounds, we addressed three questions to test the neutral, background heterogeneity, and differential crypsis hypotheses in four marking-type morphs: 1) do the morphs differ in the degree of crypsis in each microhabitat, 2) are different morphs most cryptic in specific backgrounds of the microhabitats, and 3) does the morph frequency reflect the degree of crypsis? The degree of camouflage differed among the four morphs; therefore, the neutral hypothesis was rejected. Furthermore, the order of camouflage advantage among morphs differed depending on the two types of backgrounds (sand and grass), although the grass background consistently provided greater camouflage effects. Thus, based on our results, we could not reject the background heterogeneity hypothesis. Under field conditions, the more cryptic morphs comprised a minority of the population. Overall, our results demonstrate that the different morphs were not equivalent in the degree of crypsis, but the degree of camouflage of the morphs was not consistent with the morph frequency. These findings suggest that trade-offs exist between the camouflage benefit of body colouration and other fitness components, providing a better understanding of

  14. Camouflage effects of various colour-marking morphs against different microhabitat backgrounds in a polymorphic pygmy grasshopper Tetrix japonica.

    Directory of Open Access Journals (Sweden)

    Kaori Tsurui

    2010-07-01

    Full Text Available Colour-marking polymorphism is widely distributed among cryptic species. To account for the adaptive significance of such polymorphisms, several hypotheses have been proposed to date. Although these hypotheses argue over the degree of camouflage effects of marking morphs (and the interactions between morphs and their microhabitat backgrounds, as far as we know, most empirical evidence has been provided under unnatural conditions (i.e., using artificial prey.Tetrix japonica, a pygmy grasshopper, is highly polymorphic in colour-markings and occurs in both sand and grass microhabitats. Even within a microhabitat, T. japonica is highly polymorphic. Using humans as dummy predators and printed photographs in which various morphs of grasshoppers were placed against different backgrounds, we addressed three questions to test the neutral, background heterogeneity, and differential crypsis hypotheses in four marking-type morphs: 1 do the morphs differ in the degree of crypsis in each microhabitat, 2 are different morphs most cryptic in specific backgrounds of the microhabitats, and 3 does the morph frequency reflect the degree of crypsis?The degree of camouflage differed among the four morphs; therefore, the neutral hypothesis was rejected. Furthermore, the order of camouflage advantage among morphs differed depending on the two types of backgrounds (sand and grass, although the grass background consistently provided greater camouflage effects. Thus, based on our results, we could not reject the background heterogeneity hypothesis. Under field conditions, the more cryptic morphs comprised a minority of the population. Overall, our results demonstrate that the different morphs were not equivalent in the degree of crypsis, but the degree of camouflage of the morphs was not consistent with the morph frequency. These findings suggest that trade-offs exist between the camouflage benefit of body colouration and other fitness components, providing a better

  15. Identification, functional characterization and expression pattern of myeloid differentiation factor 88 (MyD88) in Sepiella japonica.

    Science.gov (United States)

    Huo, Liping; Bao, Miaomiao; Lv, Zhenming; Chi, Changfeng; Wang, Tianming; Liu, Huihui

    2018-05-01

    Myeloid differentiation factor 88 (MyD88) is an adaptor protein involved in the interleukin-1 receptor and Toll-like receptor-induced activation of nuclear factor-κB (NF-κB). In this study a novel isoform of MyD88 in Sepiella japonica (SjMyD88) was cloned and functionally characterized (GenBank accession no. AQY56781.1). The complete cDNA sequence of SjMyD88 was 1912 bp and contained a 1017 bp open reading frame encoding 338 amino acid residues, which was similar to its mollusk orthologues in the length. BLASTp analysis suggested the deduced amino acids sequence of SjMyD88 shared high identity to the known MyD88, for instance, 64% identity with Octopus bimaculoides. Sequence analysis revealed two conserved domains, the N-terminal DD and the C-terminal TIR domain appeared in SjMyD88, which was consistent with MyD88 proteins from other species. The fusion expression of SjMyD88 and green fluorescent protein (EGFP) in HEK293 cells was conducted and cytoplasm localization was detected. Meanwhile, the TIR-pmCherry fusion protein showed red fluorescence and mainly distributed in the cytoplasm. After cotransfection MyD88-EGFP and TIR-pmCherry red obviously overlapped and changed to yellowish green. The results suggested that there was the interaction between homologous TIR-pmcherry and MyD88-EGFP. Tissues expression profiles analysis showed that SjMyD88 ubiquitously expressed in all tested tissues with the highest expression in the gills and livers except reproductive related tissue, and it was significantly induced in livers under LPS stress. These data provide insight into the roles of SjMyD88 in the TLR signaling pathway of S. japonica in response to pathogenic bacteria. Copyright © 2018. Published by Elsevier Ltd.

  16. Producción de biogás a partir de residuos organicos en biodigestores de bajo coste

    OpenAIRE

    Ferrer Martí, Ivet; Uggetti, Enrica; Poggio, Davide; Velo García, Enrique

    2015-01-01

    PRODUCCIÓN DE BIOGÁS A PARTIR DE RESIDUOS ORGANICOS EN BIODIGESTORES DE BAJO COSTE Ivet Ferrer*, Enrica Uggetti**, Davide Poggio***, Enric Velo**** Grup de Recerca en Cooperació i Desenvolupament Humà C. Jordi Girona, 31 08034 - Barcelona, Spain Phone: +34 93 401 64 63 Pàgina web: http://www.upc.edu/grecdh *, **, ***, **** Palabras clave: Digestión anaerobia; Biodigestor; Biogás; Energía; Fertilizante. RESUMEN ...

  17. Jenis dan Status Konservasi Ikan Hiu yang Tertangkap di Tempat Pelelangan Ikan (TPI Labuan Bajo, Manggarai Barat, Flores

    Directory of Open Access Journals (Sweden)

    Ismail Syakurachman Alaydrus

    2016-03-01

    Full Text Available The study aimed to determine the types of sharks are caught, sold and conservation status in the fish auction place (TPI Labuan Bajo, West Manggarai, Flores, East Nusa Tenggara have been conducted in February and March 2014 in Labuan Bajo. The research method was the survey method and used Market Survey sampling techniques and identification by Rapid Assessment method. Descriptively analyzed data, displayed in the form of narration and a table. Based on the results of the study, found 114 individual sharks, 55 individual males, 59 females were classified into three orders, namely Orectolobiformes, Lamniformes, and Carcharhiniformes; 4 families that Carcharhinidae, Odontaspididae, Rhynchobatidae, and Ginglymostomatidae; 5 genera namely Carcharhinus, Triaenodon, Rhyncobatus, Prionace, and Rhizoprionodon and 9 types of sharks that Carcharhinus melanopterus, Triaenodon obesus, Carcharhinus limbatus, Carcharhinus taurus, Carcharhinus amblyrhynchos, Rhynchobatus australiae, Prionace glauca, Rhizoprionodon acutus, and Nebrius ferrugineus. Based on the state of conservation, shark found in Labuan Bajo TPI classified Vulnerable and Near Threatened, the dominant type of shark found is Carcharhinus melanopterus (91 fishes while the least was found is Rhynchobatus australiae, Prionace glauca, Rhizoprionodon acutus, and Nebrius ferrugineus (each 1 individual.

  18. Microbiological corrosion in low carbon steels; Corrosion microbiologica en aceros de bajo carbono

    Energy Technology Data Exchange (ETDEWEB)

    Medina-Custodio, O; Ortiz-Prado, A; Jacobo-Armendariz, V. H; Schouwenaars-Franssens, R [Universidad Nacional Autonoma de Mexico, Mexico, D.F. (Mexico)]. E-mail: medina_1979@yahoo.com; armandoo@servidor.unam.mx; vjacobo@dgapa.unam.mx; raf_schouweenaars@yahoo.com

    2009-01-15

    The Microbiologically Induced Corrosion affects several industries, such as oil industry where it is estimated that 20% to 30% pipes failures are related with microorganism. The chemical reactions generate ions transfer, this validate the use of electrochemical technique for its analysis. Coupons submerged in a nutritional medium with presence and absence of three different microorganisms during two periods, 48 hours and 28 days were studied. Polarization resistance (Rp) and Electrochemical Impedance Spectroscopy (EIS) techniques were applied to determine the corrosively of the systems. The results show a greater corrosive effect of abiotic systems, this indicates a microorganisms protection effect to the metal, opposite to the first hypothesis. This result was ratified observing surface coupons by using Scanning Electron Microscopy (SEM) technique. A possible mechanism based on Evans - Tafel graph is proposed to explain inhibitor microorganism effect. [Spanish] La corrosion microbiologica es un tipo comun de deterioro que afecta diversas industrias, una de ellas es la petrolera en la que se estiman que el 20% o 30% de fallas en las tuberias de trasporte de hidrocarburos es favorecida por microorganismos. Las reacciones quimicas que sustentan estos, generan transferencia de iones, lo que justifica el empleo de tecnicas electroquimicas para su analisis. En este trabajo, se estudiaron probetas de acero de bajo carbono SAE 1018, sumergidas en un medio nutritivo rico en cloruros en presencia y ausencia de tres diferentes cargas microbianas, en tiempos de exposicion de 48 horas y 28 dias. Se realizaron ensayos de resistencia a la polarizacion (Rp) y espectroscopia de impedancia electroquimica (EIS) para determinar el efecto corrosivo de los diferentes sistemas. Los resultados muestran que el medio abiotico causa el mayor efecto corrosivo, lo que indica un efecto protector de los microorganismos al metal contradiciendo la hipotesis inicialmente propuesta. La observacion

  19. Especies forrajeras utilizadas bajo silvo-pastoreo en el centro de Chiapas

    Directory of Open Access Journals (Sweden)

    R. Pinto

    2004-01-01

    Full Text Available Con el objetivo de conocer los usos, la composición química y degradabilidad ruminal de especies arbóreas y herbáceas del trópico mexicano, se desarrolló el presente trabajo en el Valle Central del estado de Chiapas, México. A través de entrevistas con productores se identificaron 14 leñosas y 7 herbáceas con mayor mención forrajera, así como sus múltiples usos. De éstas, el contenido de PC varió de medio a alto (5.8-28.7%, sobresaliendo las especies herbáceas, el contenido de MO fue muy similar entre especies (79.1 96.6% y los niveles más altos de FDN y FDA se encontraron en los frutos y las mayores concentraciones de FT en los follajes arbóreos. En términos de degradabilidad a 24 h de la PC, MS y MO, sobresalieron el follaje de G. americana y el fruto de E. cyclocarpum (P<0.05, en tanto, el follaje y fruto de A. pennatula, y el follaje de B. ungulata y M. hondurana presentaron los valores más bajos (P<0.05 de todas las especies en evaluación. Los parámetros de degradabilidad ruminal (a, b, a+b, c de los frutos fueron menores en comparación a los follajes. Sobresalió el fruto de E. cyclocarpum por su alto potencial de degradabilidad. Para el caso del follaje, excepto el género Acacia, todas las especies presentaron valores altos de degradabilidad ruminal de la MS

  20. El ideal de Yo bajo la tutela del Superyó.

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    María Paulina Mejía.

    1999-01-01

    Full Text Available Este texto se ocupará de analizar la naturaleza y función del Ideal del Yo, así como s relación con la instancia del superyó. Para tal fin se retomará el texto Introducción al Narcisismo, en el cual Freud introduce el Superyó con el nombre de conciencia moral y en el que, a su vez, explica el origen del ideal del Yo como el heredero de la ley y el nacimiento de del “juicio propio”. El ideal del Yo y el Superyó, ambos herederos del Complejo de Edipo, tienen en común que, si bien son resultado de la intervención de la ley y se valen del lenguaje, no se acogen a su estructura y a sus leyes, es decir, que no funcionan con la lógica de la falta y privilegian significantes unarios, que por estar solos no posibilitan la construcción de sentido.En síntesis, este artículo pretende demostrar cómo el Ideal del Yo, bajo la tutela del Superyó, logra darle respuestas al sujeto en relación al ser hombre y ser mujer pero del lado del goce. La lógica que se contrapone a la dimensión del lenguaje y el deseo, los cuales se estructuran a partir de la falta. El ser desde esta dimensión deja de ser una certeza para constituirse en una pregunta

  1. Estimación del tiempo de desarrollo de Lutzomyia evansi bajo condiciones experimentales

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    Claudia Martínez Suárez

    2012-01-01

    Full Text Available Objetivo: Estimar el tiempo promedio de desarrollo de Lutzomyia evansi. Materiales y métodos: Se inició una colonia de Lutzomyia evansi con individuos recolectados en la zona urbana de la ciudad de Sincelejo (Colombia. La colonia fue mantenida en el laboratorio durante tres generaciones filiales bajo condiciones experimentales promedio de 26ºC de temperatura y 94% de humedad relativa. Resultados: La duración del desarrollo de Lutzomyia evansi fue de 36 a 45 días. El tiempo requerido para el desarrollo de los huevos fue en promedio de 6,75 días (rango de 6 a 8 días. La duración en promedio de los diferentes estadios larvales fue 5,75 días en larvas de primer estadio (rango de 5 a 8 días, 5,75 días en larvas de segundo estadio (rango de 4 a 7 días, 5 días en larvas de tercer estadio (rango de 4 a 7 días y 7 días en larvas de cuarto estadio (rango de 6 a 8 días. En la fase de pupa, la duración en promedio fue de 9,75 días (rango de 7 a 17 días. Conclusiones: El tiempo promedio requerido para el desarrollo de Lutzomyia evansi, comprendido desde la alimentación sanguínea de la hembra madre hasta la emergencia del adulto, es de 40 días.

  2. Condiciones de trabajo, fatiga laboral y bajo peso al nacer en vendedoras ambulantes

    Directory of Open Access Journals (Sweden)

    Hernández-Peña Patricia

    1999-01-01

    Full Text Available Objetivo. Este trabajo tipifica las condiciones demográficas, socioeconómicas, laborales, de fatiga laboral y daño reproductivo en una población femenina en edad fértil que trabaja en la venta ambulante en las calles de la Ciudad de México. Material y métodos. Se entrevistaron 426 vendedoras ambulantes. Se describió a la población entrevistada y se exploró la asociación de los componentes de la fatiga laboral con el bajo peso al nacer (BPN mediante regresión logística, en un subgrupo de mujeres que laboraron en la venta ambulante durante su última gestación. Resultados. De las mujeres entrevistadas, 56% realiza jornadas de trabajo superiores a las 48 horas semanales, 87% carece de seguridad social y 68% tiene estudios no mayores de primaria. El riesgo de presentar BPN se eleva cuando se carece de control sobre la cantidad de mercancía a vender (RM 6.5, IC95% 1.3-31, se venden artículos de ocasión como refacciones y ornamentos (RM 6.3, IC95% 1.5-26, se exhibe la mercancía sobre el piso o se carga para su venta (RM 7.7, IC95% 1.8-32, y cuando los recursos para instalar el puesto por primera vez no proceden de la red social de apoyo de la vendedora (RM 7.4, IC95% 1.2-44. Conclusiones. Estos resultados contribuyen a identificar a las vendedoras con mayor riesgo de presentar un hijo con BPN, y sugerir medidas preventivas.

  3. CONTAMINACIÓN DEL SUELO EN LA ZONA MINERA DE RASGATÁ BAJO (TAUSA. MODELO CONCEPTUAL

    Directory of Open Access Journals (Sweden)

    María Eugenia Guerrero Useda

    2016-01-01

    Full Text Available Se desarrolló un modelo conceptual del riesgo de contaminación físico química del suelo en la vereda Rasgatá Bajo. Esta vereda del municipio de Tausa, en Cundinamarca, está en la zona limítrofe del declarado por la Corporación Autónoma Regional de Cundinamarca en 2009, Distrito Regional de Manejo Integrado de los Recursos Naturales Renovables Páramo Guargua y Laguna Verde. La vocación productiva local por tradición ha sido el cultivo de papa (Solanum tuberosum L y la extracción y transformación de arcilla y carbón, actividades con graves impactos sobre el suelo, por lo que el objetivo del proyecto fue identificar y evaluar la condición de este recurso en territorios donde confluyen actividades de extracción y transformación de arcilla y carbón. Se siguió un enfoque metodológico mixto que combinó investigación documental, observaciones in situ y medición de características físico químicas para determinar las fuentes y tipos de contaminantes con riesgo de afectar el suelo aledaño a las faenas mineras y con impactos toxicológicos para las personas, la fauna y la flora. Los datos obtenidos aportarán al rediseño y evaluación de los planes de manejo ambiental local.

  4. Estudio de materiales adsorbentes de bajo costo para remover Cr(VI de efluentes acuosos

    Directory of Open Access Journals (Sweden)

    Claudia Vargas-Niño

    2011-01-01

    Full Text Available El presente estudio muestra el enorme potencial de dos tipos de sólidos diferentes, una alúmina comercial y un material producto del compostaje, en la adsorción/eliminación de Cr(VI en medio acuoso, en concentraciones de metal equivalentes a las halladas en aguas residuales provenientes de industrias en Colombia. Ambos materiales mostraron propiedades importantes para la eliminación de Cr(VI, considerándose de mayor relevancia el compost por ser un material de bajo costo. Se establecieron las condiciones óptimas para la adsorción de cromo sobre alúmina y sobre compost. Para la alúmina: concentración inicial de Cr(VI = 10 mgL-1, volumen de solución por masa de adsorbente = 100 mL/g, pH = 2,0, tiempo de equilibrio = 1 hora, y agitación = 150 rpm. Para el compost: concentración inicial de Cr(VI = 3 mg L-1, volumen de solución por masa de adsorbente = 50 mL/g, pH = 2.5, tiempo de equilibrio = 3 horas, y agitación = 150 rpm. Además, los resultados mostraron que las propiedades de adsorción del compost se pueden mejorar mediante la adición de pequeñas cantidades de alúmina. El estudio permitió seleccionar al compost como un material promisorio para procedimientos de remediación de aguas residuales que contienen cromo (VI dentro de una política de manejo y uso de residuos sólidos con el objeto de minimizar el impacto ambiental de éstos.

  5. Desarrollo post-embrionario de Microcerella acrydiorum (Diptera: Sarcophagidae bajo condiciones de laboratorio

    Directory of Open Access Journals (Sweden)

    Andrea V. DE ARRIBA

    2006-01-01

    Full Text Available Se estudió el desarrollo post-embrionario de Microcerella acrydiorum (Weyenbergh bajo condiciones controladas (temperatura media: 27,50 ± 2,60°C, humedad relativa media: 36,47 ± 8,10% y fotoperíodo artificial: 14 horas luz y se determinaron los siguientes parámetros biológicos: tiempo de desarrollo larval y pupal, período de prelarviposición, supervivencia y razón sexual. El tiempo de desarrollo larval fue de 3,78 ± 0,81 días, el tiempo de desarrollo pupal de 8,63 ± 1,45 días, el período de prelarviposición de 18,25 ± 4,06 días y el ciclo completo fue de 30,66 días. La probabilidad de supervivencia al estado adulto fue de 75,80%. Al menos el 98,73% de las larvas lograron formar el pupario, de estas el 85,81% completó la metamorfosis y transformadas en adultos abandonaron los puparios; sólo el 89,47% de los adultos emergió a la superficie. El estado pupal resultó ser el más susceptible, concentrando casi el 58% de las muertes. La proporción de sexos no se apartó de la relación esperada 1:1 (p > 0,15.

  6. Vivencia infantil del clima de violencia y represión bajo dictadura

    Directory of Open Access Journals (Sweden)

    Salomón Magendzo

    2000-01-01

    Full Text Available Esta investigación parte del supuesto de que el régimen dictatorial chileno no sólo afectó a los niños y niñas cuyos familiares fueron directamente detenidos, torturados y/o desaparecidos, sino también a aquellos que sólo tuvieron que vivir el clima de represión masiva, especialmente en los barrios de bajos recursos. La mayor parte de los jóvenes entrevistados recordaron espontáneamente el clima caracterizado por protestas de la población y represión intensa durante el período 1983-1986. Las entrevistas fueron analizadas a través de un método inductivo, sin categorías preestablecidas. Partiendo del relato de los propios jóvenes sobre su infancia, se identificaron los siguientes ejes que permitieron ordenar la información: la descripción del clima de violencia y represión; el fenómeno de la vivencia emocional de dicho clima como niños y niñas; las reacciones familiares frente al referido clima; la visión de la infancia, la familia y la población que los entrevistados perciben que tuvieron como niños y niñas; las consecuencias del haber vivido en un clima de violencia y represión a nivel personal y social; y la relación de los jóvenes con sus recuerdos, así como la visión actual que tienen de su población. El artículo da cuenta de lo encontrado en los ejes mencionados para luego extenderse a una discusión acerca de los resultados.

  7. Uso eficiente del agua para el cultivo de rosa cv. Freedom bajo invernadero

    Directory of Open Access Journals (Sweden)

    John J. Arévalo-H

    2013-08-01

    Full Text Available Determinar el requerimiento de agua y el momento adecuado de distribución, es un factor fundamental para el cultivo de flores. El objetivo del presente trabajo fue determinar el efecto de la aplicación de tres láminas de riego sobre el desarrollo y producción del cultivo de la Rosa cv. Freedom, bajo invernadero. El experimento se realizó con tres láminas de riego en un cultivo de rosa (Rosa spp. cv. Freedom. Se analizó el crecimiento de los tallos florales, la vida en florero, la producción y la eficiencia en el uso del agua. Los tratamientos se basaron en tres niveles de evapotranspiración de cultivo (ETc (70, 80 y 100%. Durante el estudio, el cultivo se mantuvo en producción constante. Se aplicaron 384,87, 254,25 y 218,97 m³ correspondiente a 100, 80 e 70% de ETc respectivamente, significando un ahorro de agua de 14,0 y 21,8% en el 80 e 70% de ETc respectivamente. El coeficiente de cultivo (Kc vario entre 0,58 y 1,65, con una media de 1,13. No se encontraron diferencias en la producción. Las curvas de crecimiento presentaron incremento continuo, con ajuste al modelo logístico. El 70% de ETc presentó la mayor eficiencia en el uso del agua, con 185 tallos m-3. La conservación media de los tallos florales fue de 12 a 15 días. La mayor causa de pérdida fue la deshidratación, que inició en el día 11, comportamiento normal del tallo floral.

  8. NOAA ESRI Geotiff - 5m Bathymetry around Bajo de Cico, Puerto Rico, Project NF-07-06, 2007, UTM 19 NAD 83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains an ESRI Geotiff with 5 meter cell size representing the bathymetry of selected portions of seafloor around Bajo De Cico in Puerto Rico, derived...

  9. NOAA TIFF Image - 1 m Backscatter Mosaic of Bajo de Cico, Puerto Rico, Project NF-07-06, 2007, UTM 19 NAD83 (NCEI Accession 0131853)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This image represents a 1 meter resolution backscatter mosaic of Bajo de Cico off the coast of western Puerto Rico, collected using a Kongsberg EM 1002 (95 kHz)...

  10. NOAA ESRI Grid - 3m Bathymetry around Bajo de Cico, Puerto Rico, Project NF-07-06, 2007, UTM 19 NAD 83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains an ESRI Grid with 3 meter cell size representing the bathymetry of selected portions of seafloor around Bajo De Cico in Puerto Rico, derived...

  11. NOAA ESRI Grid - 5m Bathymetry around Bajo de Cico, Puerto Rico, Project NF-07-06, 2007, UTM 19 NAD 83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains an ESRI Grid with 5 meter cell size representing the bathymetry of selected portions of seafloor around Bajo De Cico in Puerto Rico, derived...

  12. NOAA ESRI Geotiff - 3m Bathymetry around Bajo de Cico, Puerto Rico, Project NF-07-06, 2007, UTM 19 NAD 83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains an ESRI Geotiff with 3 meter cell size representing the bathymetry of selected portions of seafloor around Bajo De Cico in Puerto Rico, derived...

  13. NOAA ESRI Geotiff - 10m Bathymetry around Bajo de Cico, Puerto Rico, Project NF-07-06, 2007, UTM 19 NAD 83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains an ESRI Geotiff with 10 meter cell size representing the bathymetry of selected portions of seafloor around Bajo De Cico in Puerto Rico,...

  14. Influence of water temperature and feeding regime on otolith growth in Anguilla japonica glass eels and elvers: does otolith growth cease at low temperatures?

    Science.gov (United States)

    Fukuda, N; Kuroki, M; Shinoda, A; Yamada, Y; Okamura, A; Aoyama, J; Tsukamoto, K

    2009-06-01

    The influences of water temperature and feeding regime on otolith growth in Anguilla japonica glass eels and elvers were investigated using individuals reared at 5, 10, 15, 20, 25 and 30 degrees C and in fed or unfed conditions at salinity 32 after their otoliths were marked with alizarin complexone (ALC). To eliminate the difficulty of observing the edges of otoliths with optical (OM) or scanning electron (SEM) microscopes, three to 10 individuals were sampled from each tank at 10, 20 and 30 days during the experiment and reared for an additional 10 days at 25 degrees C after their otoliths were marked a second time. Otolith growth and the number of increments were measured using both OM and SEM. Most A. japonica commenced feeding after 10 days at 20-30 degrees C or after 20 days at 15 degrees C, but no feeding occurred at 5 and 10 degrees C. No otolith growth occurred at 5 and 10 degrees C except in two individuals with minimal increment deposition at 10 degrees C. Otolith growth was proportional to water temperature within 15-25 degrees C and not different between 25 and 30 degrees C. At 15, 25 and 30 degrees C, the mean otolith growth rate in fed conditions was higher than in unfed conditions. The number of increments per day was significantly different among water temperatures (0.00-0.01 day(-1) at 5 and 10 degrees C, 0.43-0.48 day(-1) at 15 degrees C and 0.94-1.07 day(-1) at 20-30 degrees C). These results indicated that otolith growth in A. japonica glass eels and elvers was affected by temperature and ceased at otoliths of wild-caught A. japonica glass eels and elvers need to carefully consider the water temperatures potentially experienced by the juveniles in the wild.

  15. Riesgos y consecuencias de las prácticas sexuales en adolescentes bajo los efectos de alcohol y otras drogas

    Directory of Open Access Journals (Sweden)

    Guillermo Castaño Pérez

    2013-03-01

    Full Text Available Introducción: la sexualidad, y el consumo de alcohol y drogas en los adolescentes, figuran en la actualidad como uno de los asuntos con mayor prioridad para la salud pública. La preocupación radica en reducir los riesgos de infecciones de transmisión sexual, el virus de la inmunodeficiencia humana y los embarazos tempranos no deseados. Objetivo: analizar las prácticas sexuales bajo el efecto de alcohol y otras drogas, y los riesgos que esto implica, en los adolescentes de la ciudad de Medellín. Métodos: la muestra se estableció mediante método probabilístico aleatorio y por conglomerados. El universo correspondió al número total de estudiantes matriculados en colegios públicos y privados de la ciudad de Medellín de 8vo., 9no., 10mo. y 11no. grados, en el año 2010 (91 857 estudiantes con edades comprendidas entre los 14 y 17 años. El tamaño muestral incluido fue de 955 estudiantes usando un intervalo de confianza de 95. Se diseñó un estudio descriptivo, transversal para conocer las prácticas sexuales bajo el efecto de alcohol u otras drogas, y los riesgos que corren los adolescentes. Se establecieron frecuencias, porcentajes y asociaciones estadísticas con el programa SPSS, versión 19.0 Resultados: consumir alcohol u otras drogas y tener prácticas sexuales presenta una asociación estadística significativa (p= 0,000. El 62,4 % de los adolescentes que han consumido una sustancia psicoactiva, han tenido relaciones sexuales, frente a un 29,4 % que, habiendo consumido alcohol y otras drogas, no lo han hecho. El alcohol es la droga más usada (79,8 %. Le siguen la marihuana (31,83 %, poppers (8,97 %, la cocaína (7,75 % y el éxtasis (5,71 %. Las prácticas sexuales más frecuentes bajo el efecto de sustancias psicoactivas son las exploratorias (manoseo, caricias, para un 71,02 %, seguida de la penetrativa vaginal (63,67 %, el sexo oral (45,30 % y la masturbación (19,59 %. De los adolescentes que tienen prácticas sexuales (N

  16. Improving the quality of Laminaria japonica-based diet for Apostichopus japonicus through degradation of its algin content with Bacillus amyloliquefaciens WB1.

    Science.gov (United States)

    Wang, Xitao; Wang, Lili; Che, Jian; Li, Zhen; Zhang, Jiancheng; Li, Xiaoyu; Hu, Weiqing; Xu, Yongping

    2015-07-01

    Laminaria japonica feedstuff is used as a substitute for Sargassum thunbergii in the small-scale culturing of Apostichopus japonicus (sea cucumber) because of its abundant sources and low price in China. However, the difficulty associated with the degradation of algin by A. japonicus and, hence, its utilization have limited the practical value of L. japonica feedstuff in sea cucumber farming. In this study, A. japonicus individuals were fed with L. japonica feedstuff pretreated, via fermentation with the algin-degrading bacterial strain, Bacillus amyloliquefaciens WB1, and their growth performance, nonspecific immune responses, and resistance against Vibrio infection were then determined over a 60-day period. Growth performance of these individuals was similar to those fed with a commercial feedstuff made from S. thunbergii (mean weight gain of 5.79 versus 5.69 g on day 60), but was significantly (P content had been degraded by B. amyloliquefaciens WB1 could improve the growth performance of A. japonicus as well its resistance to bacterial infection. It could therefore act as an alternative to S. thunbergii and is economical at the same time.

  17. Haustoria of Cuscuta japonica, a holoparasitic flowering plant, are induced by the cooperative effects of far-red light and tactile stimuli

    International Nuclear Information System (INIS)

    Tada, Y.; Sugai, M.; Furuhashi, K.

    1996-01-01

    When seedlings of Cuscuta japonica were grown with Vigna radiata in a flower pot for 6 d under white light and then irradiated with far-red or blue light, the seedlings parasitized V. radiata. However, no parasitism of the seedlings was observed under red or white light or in darkness. The parasitic behavior of seedlings of C. japonica was observed even if an acrylic rod was used as a substitute for the host plant. Upon incubation under far-red light, the seedling twined tightly around the rod and developed haustoria towards it. Haustoria also developed when apical and subapical regions of seedlings were held between two glass plants that were about 0.7 mm apart and were irradiated with far-red light. However, no haustoria were induced by either the hold or irradiation alone. These results indicate that parasitism of cuscuta japonica is controlled by the cooperative effects of two physical signals, far-red light and appropriate tactile pressure. Our findings suggest that parasitism by the genus Cuscuta involves a novel strategy

  18. Effects of Surface Charge and Functional Groups on the Adsorption and Binding Forms of Cu and Cd on Roots of indica and japonica Rice Cultivars

    Directory of Open Access Journals (Sweden)

    Zhao-Dong Liu

    2017-08-01

    Full Text Available This work was designed to understand the mechanisms of adsorption of copper (Cu and cadmium (Cd on roots of indica and japonica varieties of rice. Six varieties each of indica and japonica rice were grown in hydroponics and the chemical properties of the root surface were analyzed, including surface charges and functional groups (-COO- groups as measured by the streaming potential and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR. Binding forms of heavy metals adsorbed on rice roots were identified using sequential extraction methods. In rice roots exposed to Cu and Cd solutions, Cu existed mainly in both exchangeable and complexed forms, whereas Cd existed mainly in the exchangeable form. The amounts of exchangeable Cu and Cd and total adsorbed metal cations on the roots of indica varieties were significantly greater than those on the roots of japonica varieties, and the higher negative charges and the larger number of functional groups on the roots of indica varieties were responsible for their higher adsorption capacity and greater binding strength for Cu and Cd. Surface charge and functional groups on roots play an important role in the adsorption of Cu and Cd on the rice roots.

  19. Comparison of the Trace Elements and Active Components of Lonicera japonica flos and Lonicera flos Using ICP-MS and HPLC-PDA.

    Science.gov (United States)

    Zhao, Yueran; Dou, Deqiang; Guo, Yueqiu; Qi, Yue; Li, Jun; Jia, Dong

    2018-06-01

    Thirteen trace elements and active constituents of 40 batches of Lonicera japonica flos and Lonicera flos were comparatively studied using inductively coupled plasma mass-spectrometry (ICP-MS) and high-performance liquid chromatography-photodiode array (HPLC-PDA). The trace elements were 24 Mg, 52 Cr, 55 Mn, 57 Fe, 60 Ni, 63 Cu, 66 Zn, 75 As, 82 Se, 98 Mo, 114 Cd, 202 Hg, and 208 Pb, and the active compounds were chlorogenic acid, 3,5-O-dicaffeoylquinc acid, 4,5-O-dicaffeoylquinc acid, luteolin-7-O-glucoside, and 4-O-caffeoylquinic acid. The data of 18 variables were statistically processed using principal component analysis (PCA) and discriminate analysis (DA) to classify L. japonica flos and L. flos. The validated method was developed to divide the 40 samples into two groups based on the PCA in terms of 18 variables. Furthermore, the species of Lonicera was better discriminated by using DA with 12 variables. These results suggest that the method and statistical analysis of the contents of trace elements and chemical components can classify the L. japonica flos and L. flos using 12 variables, such as 3,5-O-dicaffeoylquincacid, luteolin-7-O-glucoside, Cd, Mn, Hg, Pb, Ni, 4-O-caffeoyl-quinic acid, 4,5-O-dicaffeoylquinc acid, Fe, Mg, and Cr.

  20. Actividad enzimática del suelo -Deshidrogenasa , -ß Glucosidasa , Fosfatasa y Ureasa - bajo diferentes cultivos.

    Directory of Open Access Journals (Sweden)

    Carlos Henríquez

    2014-01-01

    Full Text Available Se analizó la actividad de 4 enzimas del suelo en fincas bajo diferentes manejos agronómi - cos y en diferentes tipos de suelos. Las enzimas evaluadas fueron la Fosfatasa, β-Glucosidasa, Deshidrogenasa y Ureasa. Se tomaron muestras de suelo de los primeros 20 cm de profundidad con el fin de analizarlas químicamente y determinar la actividad de las 4 enzimas a evaluar. Se encontró que la actividad de la Deshidrogenasa varió de 0,13 a 4,46 con un promedio de 1,17 (μg INTF.g - 1 .h -1 ; la β-Glucosidasa varió de 31,9 a 208,1 con un promedio de 108,1 (μg PNP.g -1 .h -1 . Por otro lado la Fosfatasa tuvo valores entre 413,4 y 3043,6 con un promedio de 1521,5 μg PNP.g -1 .h -1 , mientras que la Ureasa varió de 12,5 a 52,8 con un promedio de 38,3 μg N-NH4.g -1 .h -1 . La Fos - fatasa correlacionó con el porcentaje de materia orgánica y de carbono en el suelo (0,75 y 0,76 respectivamente, p<0,01; se encontró una rela - ción inversa entre la actividad de esta enzima y el contenido de P disponible (r de -0,53, p<0,10. La Ureasa correlacionó con la Fosfatasa (r de 0,61 p<0,05 y con la Deshidrogenasa (r de -0,77 p<0,01. El análisis multivariado de conglomera - dos a partir de la actividad enzimática permitió hacer grupos similares según el tipo de cultivo, lo que evidencia el potencial de utilización que tiene esta propiedad para realizar estudios más detallados.

  1. Viabilidad de Saccharomyces boullardii en queso fresco bajo condiciones de acidez “in vitro”

    Directory of Open Access Journals (Sweden)

    Rafael Zamora-Vega

    2015-01-01

    Full Text Available Un alimento funcional contiene en su formulación una o más sustancias que generan bienestar a la salud humana como son probióticos, prebióticos y ácidos grasos, entre otros. En los alimentos funcionales los microorganismos probióticos deben permanecer viables y activos en el alimento y durante el pasaje gastrointestinal, para garantizar su potencial efecto benéfico en el huésped. En el presente estudio se evaluó la viabilidad de la levadura probiótica Saccharomyces boulardii tanto de forma libre como encapsulada bajo condiciones de acidez, adaptada a un queso fresco. Método: La levadura fue expuesta en el alimento tanto en forma libre como encapsulada. Los materiales usados para encapsular fueron una mezcla de alginato de sodio, inulina y mucílago de nopal (Opuntia ficus-indica. Para la liberación del microorganismo, el estudio se realizó a pH de 2.0 y 6.5, simulándose las condiciones de acidez de estómago y colon respectivamente, a diferentes tiempos de exposición de 0, 60, 120 y 180 min. Resultados: A pH de 2.0 se observó mayor pérdida en la viabilidad del probiótico en forma libre, la cual fue de 23.72, 27.03 y 33.02 % respectivamente, con respecto a la viabilidad inicial; cuando el microorganismo se adicionó al queso en forma encapsulada, su pérdida de viabilidad a los mismos tiempos de exposición fue de 5.74, 14.24 y 18.81%, manteniendo mayor supervivencia. Por otra parte, a pH de 6.5 el probiótico libre en el alimento mostró una pérdida de viabilidad de 2.23, 3.50 y 5.94%, en cambio de forma encapsulada la pérdida de viabilidad fue de 0.95, 2.20 y 3.03%, respectivamente, observándose mayor supervivencia que en estado libre. Conclusión: El queso fresco mantuvo viable el nivel de supervivencia de Saccharomyces boulardii, particularmente cuando fue adicionado en forma encapsulada comparado a cuando fue incorporado en estado libre.

  2. La Frontera como Recurso Turístico. Posibilidades del Bajo/Baixo Guadiana

    Directory of Open Access Journals (Sweden)

    José Manuel Jurado-Almonte

    2018-02-01

    Full Text Available En el espacio de integración de la Unión Europea persisten las fronteras, ya sin las dificultades y barreras de antaño, pero manteniendo numerosos símbolos y elementos del patrimonio cultural que siguen marcando una diferencia. Son líneas divisorias y, a la vez, franjas de territorios de transición con un importante patrimonio natural y cultural que se convierten ahora en recursos de atracción para novedosas demandas turísticas. Por ello, se estudia ya el turismo de frontera como un nuevo producto donde el recurso de la frontera y la historia es el principal atractivo y destino, aunque de forma complementaria a otros recursos y productos turísticos. Al margen de otros territorios y destinos fronterizos de Europa o del mundo, nuestra preocupación investigadora se centra en la Raya luso-española, en su extremo sur, en el Bajo Guadiana, en espacios que participan del Algarve, Baixo Alentejo y Huelva. En este territorio se conjugan destinos turísticos consolidados (Algarve y Costa Occidental de Huelva con un interior muy próximo e ignoto para la mayor parte de los turistas alojados en ese borde costero, a apenas unas decenas de kilómetros. Sin embargo, en los últimos años, un incipiente espacio turístico parece abrirse tierras adentro (y aguas desde la costa. El estuario del Guadiana actúa de puerta de entradahacia un interior rico en recursos y patrimonios histórico-artísticos y naturales y que podría favorecerse de un turismo de frontera. Nuestra hipótesis principal es que el desarrollo del turismo de frontera podría ser una oportunidad para este territorio transfronterizo. En este trabajo tratamos, por tanto, de: -\tAnalizar el marco geográfico y el patrimonio natural y cultural de este ámbito transfronterizo, con una breve semblanza a su población y políticas de cooperación en materia de infraestructuras de accesibilidad y de turismo. -\tdiagnosticar este novedoso producto turístico y para este territorio en

  3. Mecanismo de acción de las fosfatasas ácidas de bajo peso molecular

    Directory of Open Access Journals (Sweden)

    Emilio Guija

    2007-12-01

    Full Text Available Las fosfatasas ácidas son enzimas ampliamente distribuidas en la naturaleza y tienen la propiedad de hidrolizar fosfomonoésteres a pH 5,0, liberando como productos de la reacción un alcohol y fosfato inorgánico. Cuando se compara los valores de kcat/Km de las fosfatasas de hígado, de bovino, alpaca y porcino, nos permite sugerir que estas enzimas tienen elevada afinidad por el sustrato p-nitrofenil fosfato. Los productos que se liberan durante la catálisis por fosfatasa ácida, muestran que el fenol o el p-nitrofenol se comportan como inhibidores de tipo no competitivo, mientras que el fosfato inorgánico muestra una inhibición de tipo competitiva. Para evidenciar la probable formación de un complejo covalente en la secuencia catalítica, se utilizó diversos nucleófilos más eficientes que el agua, tales como metanol, etanol y glicerol. Las modificaciones que produjeron en los valores Km, Vmax y los productos liberados en la reacción sugieren la formación de un complejo enzima-fosfato. El pH afecta los valores de Km y Vmax de las fosfatasas ácidas, un análisis del comportamiento de estas enzimas en un rango de pH comprendido entre 3,8 y 6,8 sugieren que en el sitio activo existe un residuo de aminoácido con un valor pKa de 6,0. El uso del dietilpirocarbonato, un compuesto que selectivamente reacciona con el residuo histidina en las proteínas, inhibe completamente a estas fosfatasas. Así mismo, un experimento de cinética de inhibición múltiple realizado en presencia de fosfato inorgánico y dietilpirocarbonato permite calcular un valor Ki que es igual al obtenido en otras condiciones experimentales. En tal sentido, las fosfatasas ácidas de bajo peso molecular catalizarían las reacciones a través de un modelo de tipo uni biordenado, con la formación de un complejo covalente intermedio, y que el residuo histidina participaría directamente en la catálisis.

  4. La importancia de los materiales en las viviendas de muy bajo coste

    Directory of Open Access Journals (Sweden)

    Salas, Julián

    1992-09-01

    Full Text Available This text fits into the context of the attempt to emphasize the considerable importance of the factor of the construction materials in the subsection of low cost housing in Developing Countries in general and in Latín America in particular. The author tries to quantify, in a global manner, the repercussion of the materials through different assumptions and to show how it increases as the sections of installations and finishes diminish —in fact, practically disappear—. In accordance with this and as a consequence, the author discusses the necessity to use autochthonous materials and to support the research and technological development which lay stress on the use of alternative materials and the re-use of agro-industrial waste. As a pan of the Chapter V reproduced here, a special Section entitled "The Industry of Construction Materials" is contemplated, a section which we omitted and which can be found in n.º 198 of this Quarterly where it was originally published.

    El texto que sigue se encuadra en el contexto de intentar enmarcar la importancia considerable del capítulo de los materiales de construcción en el subsector de las viviendas de muy bajo coste en los Países en Vías de Desarrollo en general, y en Latinoamérica en particular. El autor trata de cuantificar, en forma global, la repercusión de los materiales en varios supuestos y como ésta aumenta al disminuir —prácticamente desaparecer— las partidas de instalaciones y acabados. De conformidad y como consecuencia de lo anterior, el autor se ocupa de la necesidad de emplear materiales autóctonos y de apoyar la investigación y el desarrollo tecnológico que ponen énfasis en la utilización de materiales alternativos y la reutilización de desechos agro-industriales. Formando parte del Capítulo V que se reproduce, se contempla un apartado específico con el título "La Industria de Materiales de la Construcción" que hemos omitido, remitiendo al lector

  5. [Effects of low temperature in the light on antioxidant contents in rice (Oryza sativa L.) indica and japonica subspecies seedlings].

    Science.gov (United States)

    Li, Xia; Dai, Chuan-Chao; Jiao, De-Mao; Foyer, Christine H

    2006-06-01

    To study the nature and mechanisms of resistance of rice plants to chilling stress, the effects of low temperature treatment (8 degrees C) on the photosynthetic rate and some important compounds forming redox cycles were measured. The rice varieties used are two japonica rice varieties, i.e., Taipei 309 and Wuyujing; three indica rice varieties, i.e., IR64, Pusa and CA212; and one intermediate type, i.e., Shanyou 63. Three types of varieties were studied by comparing. The light intensity-photosynthesis curves, CO2-photosynthesis curves, primary photochemical efficiency (Fv/Fm), active oxygen species (AOS) (O2*- and H2O2), glutathione (both oxidized and reduced forms) and ascorbate contents in their six-week old seedlings were measured before and after chilling treatment. The results showed that relative to the rice varieties chilling tolerance such as Taipei 309 and Wuyujing, the sensitive ones indica IR64, Pusa and CA212 exhibited a stronger inhibition of maximum photosynthetic rate (Pmax) (Figs.1 and 2) and a decrease in Fv/Fm (Fig.3), which led to the accumulation of AOS (Fig.6). It was found that the glutathione disulphide (GSSG) content in glutathione pool and that of dehydroascorbate (DHA) in ascorbate pool of the leaves of these sensitive ones under chilling were induced to increase obviously (Table 3). The correlation coefficient between the increases in GSSG, DHA and the decrease of Chl content were -0.701**, -0.656** respectively (Table 4). This indicated that the regeneration of reduced glutathione (GSH) and ascorbate was inhibited, resulting in accumulations of AOS and the reduction of Chl content (Fig.4) and the inhibition of photosynthetic activity (Fig.1 and Fig.2). The changes in japonica Taibei 309 and Wuyujing were small. And the changes in indica hybrid were lying between the above-mentioned types. Particularly, the ratio of AsA/DHA and GSH/GSSG (Fig.7) showed similar changes as those in Chl content (Fig.4). The correlation coefficient among Chl

  6. Shading Contributes to the Reduction of Stem Mechanical Strength by Decreasing Cell Wall Synthesis in Japonica Rice (Oryza sativa L.

    Directory of Open Access Journals (Sweden)

    Longmei Wu

    2017-05-01

    Full Text Available Low solar radiation caused by industrial development and solar dimming has become a limitation in crop production in China. It is widely accepted that low solar radiation influences many aspects of plant development, including slender, weak stems and susceptibility to lodging. However, the underlying mechanisms are not well understood. To clarify how low solar radiation affects stem mechanical strength formation and lodging resistance, the japonica rice cultivars Wuyunjing23 (lodging-resistant and W3668 (lodging-susceptible were grown under field conditions with normal light (Control and shading (the incident light was reduced by 60% with a black nylon net. The yield and yield components, plant morphological characteristics, the stem mechanical strength, cell wall components, culm microstructure, gene expression correlated with cellulose and lignin biosynthesis were measured. The results showed that shading significantly reduced grain yield attributed to reduction of spikelets per panicles and grain weight. The stem-breaking strength decreased significantly under shading treatment; consequently, resulting in higher lodging index in rice plant in both varieties, as revealed by decreased by culm diameter, culm wall thickness and increased plant height, gravity center height. Compared with control, cell wall components including non-structural carbohydrate, sucrose, cellulose, and lignin reduced quite higher. With histochemical straining, shading largely reduced lignin deposition in the sclerenchyma cells and vascular bundle cells compared with control, and decreased cellulose deposition in the parenchyma cells of culm tissue in both Wuyunjing23 and W3668. And under shading condition, gene expression involved in secondary cell wall synthesis, OsPAL, OsCOMT, OsCCoAOMT, OsCCR, and OsCAD2, and primary cell wall synthesis, OsCesA1, OsCesA3, and OsCesA8 were decreased significantly. These results suggest that gene expression involved in the reduction of

  7. iTRAQ-based proteome profile analysis of superior and inferior Spikelets at early grain filling stage in japonica Rice.

    Science.gov (United States)

    You, Cuicui; Chen, Lin; He, Haibing; Wu, Liquan; Wang, Shaohua; Ding, Yanfeng; Ma, Chuanxi

    2017-06-07

    Large-panicle rice varieties often fail to achieve their yield potential due to poor grain filling of late-flowering inferior spikelets (IS). The physiological and molecular mechanisms of poor IS grain filling, and whether an increase in assimilate supply could regulate protein abundance and consequently improve IS grain filling for japonica rice with large panicles is still partially understood. A field experiment was performed with two spikelet removal treatments at anthesis in the large-panicle japonica rice line W1844, including removal of the top 1/3 of spikelets (T1) and removal of the top 2/3 of spikelets (T2), with no spikelet removal as a control (T0). The size, weight, setting rate, and grain filling rate of IS were significantly increased after spikelet removing. The biological functions of the differentially expressed proteins (DEPs) between superior and inferior spikelets as well as the response of IS to the removal of superior spikelets (SS) were investigated by using iTRAQ at 10 days post anthesis. A total of 159, 87, and 28 DEPs were identified from group A (T0-SS/T0-IS), group B (T0-SS/T2-IS), and group C (T2-IS/T0-IS), respectively. Among these, 104, 63, and 22 proteins were up-regulated, and 55, 24, and 6 proteins were down-regulated, respectively. Approximately half of these DEPs were involved in carbohydrate metabolism (sucrose-to-starch metabolism and energy metabolism) and protein metabolism (protein synthesis, folding, degradation, and storage). Reduced endosperm cell division and decreased activities of key enzymes associated with sucrose-starch metabolism and nitrogen metabolism are mainly attributed to the poor sink strength of IS. In addition, due to weakened photosynthesis and respiration, IS are unable to obtain a timely supply of materials and energy after fertilization, which might be resulted in the stagnation of IS development. Finally, an increased abundance of 14-3-3 protein in IS could be involved in the inhibition of starch

  8. Shading Contributes to the Reduction of Stem Mechanical Strength by Decreasing Cell Wall Synthesis in Japonica Rice (Oryza sativa L.).

    Science.gov (United States)

    Wu, Longmei; Zhang, Wujun; Ding, Yanfeng; Zhang, Jianwei; Cambula, Elidio D; Weng, Fei; Liu, Zhenghui; Ding, Chengqiang; Tang, She; Chen, Lin; Wang, Shaohua; Li, Ganghua

    2017-01-01

    Low solar radiation caused by industrial development and solar dimming has become a limitation in crop production in China. It is widely accepted that low solar radiation influences many aspects of plant development, including slender, weak stems and susceptibility to lodging. However, the underlying mechanisms are not well understood. To clarify how low solar radiation affects stem mechanical strength formation and lodging resistance, the japonica rice cultivars Wuyunjing23 (lodging-resistant) and W3668 (lodging-susceptible) were grown under field conditions with normal light (Control) and shading (the incident light was reduced by 60%) with a black nylon net. The yield and yield components, plant morphological characteristics, the stem mechanical strength, cell wall components, culm microstructure, gene expression correlated with cellulose and lignin biosynthesis were measured. The results showed that shading significantly reduced grain yield attributed to reduction of spikelets per panicles and grain weight. The stem-breaking strength decreased significantly under shading treatment; consequently, resulting in higher lodging index in rice plant in both varieties, as revealed by decreased by culm diameter, culm wall thickness and increased plant height, gravity center height. Compared with control, cell wall components including non-structural carbohydrate, sucrose, cellulose, and lignin reduced quite higher. With histochemical straining, shading largely reduced lignin deposition in the sclerenchyma cells and vascular bundle cells compared with control, and decreased cellulose deposition in the parenchyma cells of culm tissue in both Wuyunjing23 and W3668. And under shading condition, gene expression involved in secondary cell wall synthesis, OsPAL, OsCOMT, OsCCoAOMT, OsCCR , and OsCAD2 , and primary cell wall synthesis, OsCesA1, OsCesA3 , and OsCesA8 were decreased significantly. These results suggest that gene expression involved in the reduction of lignin and

  9. An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice

    Directory of Open Access Journals (Sweden)

    Jia Yulin

    2007-06-01

    Full Text Available Abstract Background Plants respond to low temperature through an intricately coordinated transcriptional network. The CBF/DREB-regulated network of genes has been shown to play a prominent role in freeze-tolerance of Arabidopsis through the process of cold acclimation (CA. Recent evidence also showed that the CBF/DREB regulon is not unique to CA but evolutionarily conserved between chilling-insensitive (temperate and chilling-sensitive (warm-season plants. In this study, the wide contrast in chilling sensitivity between indica and japonica rice was used as model to identify other regulatory clusters by integrative analysis of promoter architecture (ab initio and gene expression profiles. Results Transcriptome analysis in chilling tolerant japonica rice identified a subset of 121 'early response' genes that were upregulated during the initial 24 hours at 10°C. Among this group were four transcription factors including ROS-bZIP1 and another larger sub-group with a common feature of having as1/ocs-like elements in their promoters. Cold-induction of ROS-bZIP1 preceded the induction of as1/ocs-like element-containing genes and they were also induced by exogenous H2O2 at ambient temperature. Coordinated expression patterns and similar promoter architectures among the 'early response' genes suggest that they belong to a potential regulon (ROS-bZIP – as1/ocs regulatory module that responds to elevated levels of ROS during chilling stress. Cultivar-specific expression signatures of the candidate genes indicate a positive correlation between the activity of the putative regulon and genotypic variation in chilling tolerance. Conclusion A hypothetical model of an ROS-mediated regulon (ROS-bZIP – as1/ocs triggered by chilling stress was assembled in rice. Based on the current results, it appears that this regulon is independent of ABA and CBF/DREB, and that its activation has an important contribution in configuring the rapid responses of rice seedlings

  10. Comparative analysis between the cost of energy for irrigation the production of Zoysia grass (Zoysia japonica Steud.); Analise comparativa entre os custos de energia na irrigacao para a producao da grama-esmeralda (Zoysia japonica Steud.)

    Energy Technology Data Exchange (ETDEWEB)

    Mazaroni, Bruno Henrique Silveira; Turco, Jose Eduardo Piteli; Coan, Ruchele Marchiori; Gerolineto, Eduardo [Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP (Brazil). Fac. de Ciencias Agrarias e Veterinarias. Dept. de Engenharia Rural

    2009-07-01

    This study analyzed the cost of energy (electrical and diesel) in the irrigated cultivation of zoysiagrass (Zoysia japonica Steud.) The research was conducted at Green-Park Farm, located in Pitangueiras, SP. It measured the consumption of diesel oil from a diesel engine, brand MWM, turbinate, 6 cylinder, and model D-229. The price of diesel oil was obtained from the Agencia Nacional do Petroleo (ANP). We studied the economic subtracting the result from the sale of zoysiagrass, the cost of consumption of diesel oil. It estimated the consumption of electric energy of a three-phase induction motor of 175 CV, WEG brand, coupled to a centrifugal pump Mark Peerless brand, model XHE-5A. To determine the energy expenditure was obtained the value of the rate with the Companhia Paulista de Forca e Luz (CPFL). A comparison of costs was performed by subtracting the economic result from the sale of grass in the cost of energy consumption. The 'Sistema Tarifario Grupo A Horo-Sazonal Verde e/ ou Azul', with special discount for irrigating at night was the lowest cost of electricity and by comparing the result of the economic costs of energy, the electrical system was more efficient in the use of fuel (diesel). (author)

  11. Factores de riesgo de bajo peso al nacer Risk factors for low birthweight

    Directory of Open Access Journals (Sweden)

    Marcelo Bortman

    1998-05-01

    Full Text Available El bajo peso al nacer (BPN es el principal factor determinante conocido de la mortalidad infantil. Pese a la notable reducción de las tasas de mortalidad infantil y al aumento de las tasas de supervivencia de niños con BPN, en Neuquén, Argentina, no se han observado descensos notables de las tasas de BPN. El propósito de este estudio fue conocer los factores de riesgo de BPN, su frecuencia en la población y el papel de la atención prenatal en su prevención, y desarrollar una escala de riesgo que permita identificar a las mujeres en mayor riesgo de dar a luz un niño de bajo peso. Con tal propósito se realizó un estudio transversal en el cual se utilizó 50% de los datos del Sistema Informático Perinatal correspondientes al período 1988­1995 y procedentes de los 29 hospitales de la provincia de Neuquén (46 171 nacimientos. Se analizaron la distribución del peso y la frecuencia de los posibles factores de riesgo de BPN. La asociación entre estos factores y el BPN se analizó mediante un modelo de regresión logística. Con los resultados obtenidos se desarrolló una escala aditiva, que fue validada con el 50% restante de los registros de nacimientos. La razón de posibilidades (RP más elevada correspondió a la falta de atención prenatal (RP = 8,78; IC95%: 6,7 a 11,4. Las RP asociadas con una atención prenatal inadecuada, una primera consulta prenatal tardía, preeclampsia o eclampsia, hemorragia o anomalías de la placenta o sus membranas e historia de un hijo previo con BPN fueron mayores de 2,0. El riesgo de tener hijos con BPN también fue más alto en las mujeres mayores de 40 años, las menores de 20, las solteras, las fumadoras, aquellas cuyo intervalo intergenésico fue menor de 18 meses y las que tenían un índice de masa corporal menor de 20. Por último, se encontró una relación lineal directa entre el puntaje en la escala y el riesgo de tener un hijo con BPN.Low birthweight (LBW is the main known determinant of

  12. Requerimiento hídrico de la berenjena Solanum melongena L. bajo

    Directory of Open Access Journals (Sweden)

    Sánchez V. Carlos

    2004-12-01

    Full Text Available

    El objetivo del presente trabajo fue determinar los requerimientos hídricos de dos genotipos de berenjena Solanum melongena L. bajo riego por goteo en el valle del Sinú medio, en época seca. El estudio se hizo en el Centro de Investigación Turipaná, de Corpoica, ubicado en Cereté, Córdoba, en un suelo clasificado como eutropet. Se utilizó un diseño de bloques al azar con un arreglo factorial de parcelas divididas, con dos genotipos de berenjena (Morada y Lila gris bajo cinco dosis de riego 1, 2, 4, 6 y 8 l∙planta -1∙día-1. Los resultados  demostraron que los genotipos de berenjena morada y lila gris se comportaron similarmente, sin presentar diferencias significativas. Hubo diferencias altamente significativas en las variables altura de planta, diámetro del tallo y rendimiento. El tratamiento de riego de 8 l∙planta

  13. Enfermedad periodontal: ¿es factor de riesgo para parto pretérmino, bajo peso al nacer o preeclampsia?

    Directory of Open Access Journals (Sweden)

    José Luis Castaldi

    2006-04-01

    Full Text Available OBJETIVOS: Evaluar si la enfermedad periodontal (EP constituye un factor de riesgo de parto pretérmino, bajo peso al nacer o preeclampsia y si hay alguna relación entre las formas clínicas de la EP y los resultados obstétricos mencionados. MÉTODOS: Estudio analítico, transversal y prospectivo de todas las mujeres que tuvieron su parto en el Hospital Dr. José Penna, de la ciudad de Bahía Blanca, Argentina, entre el 1.º de febrero y el 18 de julio de 2003 y entre el 1.º de marzo y el 31 de mayo de 2004. A las mujeres que cumplieron los criterios de inclusión (ser mayor de 18 años, tener al menos 18 dientes, no ser diabética y que los hijos hubieran sobrevivido al parto se les practicó un examen odontológico en busca de hemorragia al sondaje, movilidad dentaria o inflamación gingival y se realizaron mediciones clínicas de la pérdida de inserción. Se calcularon las razones de posibilidades (odds ratios, RP con sus intervalos de confianza del 95% (IC95% y se ajustaron los resultados por las variables "ser fumadora" y "tener anemia". RESULTADOS: Se registraron 2 003 nacimientos, correspondientes a 1 982 partos. Se excluyeron 420 (21,2% mujeres por no cumplir los criterios de inclusión o por no poder realizárseles el diagnóstico odontológico previsto. De las 1 562 mujeres evaluadas, 809 (51,8% presentaban alguna de las afecciones buscadas; de ellas, 274 (17,5% padecían EP grave y 535 (34,3% tenían gingivitis. En total, 149 (9,5% partos fueron pretérmino, en 161 (10,3% nacieron niños con bajo peso y en 157 (10,0% se presentaron casos de preeclampsia. No se observó asociación alguna entre la EP y el parto pretérmino (RP = 1,06; IC95%: 0,74 a 1,50, el bajo peso al nacer (RP = 1,05; IC95%: 0,74 a 1,47 y la preeclampsia (RP = 0,99; IC95%: 0,70 a 1,40. El riesgo de dar a luz un niño con bajo peso al nacer en el subgrupo de fumadoras de más de 10 cigarrillos diarios fue mayor en las que tenían enfermedad periodontal grave que

  14. PAUTAS PARA LA GESTIÓN INTEGRADA DE LOS RECURSOS HÍDRICOS EN LOS BAJOS SUBMERIDIONALES (ARGENTINA)

    OpenAIRE

    M. PARIS; R. P. MARANO

    2017-01-01

    La cuenca hidrográfi ca es una unidad territorial compleja tanto por sus características naturales, como por las particularidades de la sociedad. Dado que ésta es una “unidad territorial”, entonces los problemas que surjan de la gestión de los recursos hídricos serán de toda la cuenca. En el ámbito de Santa Fe está contemplado un Plan de Manejo Integral de los Bajos Submeridionales. Algunas acciones fueron llevadas a cabo en este marco, pero no hay una Agenda del Agua que sea reconocida po...

  15. Aplicación de tecnologías inalámbricas de bajo consumo para entornos exteriores ferroviarios

    OpenAIRE

    Garrido Calvo, Víctor

    2013-01-01

    El objetivo del proyecto es el análisis del funcionamiento de tecnologías inalámbricas de bajo consumo, tales como ZigBee, aplicadas en el transporte ferroviario de mercancías para la interconexión entre vagones y/o con la locomotora o vagón principal que permitan: - Localizar, posicionar e dentificar el material móvil (trenes / vagones / contenedores) y las mercancías transportadas. - Intercambiar información de manera ágil y flexible entre el material móvil y los Centros de C...

  16. Infecciones del tracto urinario bajo en adultos y embarazadas: consenso para el manejo empírico

    OpenAIRE

    Martinez, E; Osorio, J; Delgado, J; Esparza, G.E; Motoa, G; Blanco, V.M; Hernandez, C.A; Agudelo, A; Aluma, L.J; Betancurt, C.A; Ospina, W; Camargo, J.C; Canaval, H; Castañeda, C; Correa, A

    2013-01-01

    Introducción: La infección no complicada del tracto urinario bajo y la bacteriuria asintomática son causas frecuentes de consulta médica ambulatoria y en el servicio de urgencias en Colombia y el mundo. La falta de pautas y consenso para el manejo, así como la emergencia de resistencia a las múltiples opciones terapéuticas disponibles en los uropatógenos provenientes de la comunidad, hacen necesario elaborar unas recomendaciones que orienten al clínico sobre el abordaje óptimo de estas entida...

  17. Estudio embriológico de Iguana iguana (Linnaeus, 1758) bajo condiciones de incubación controladas

    OpenAIRE

    Ossa Velásquez Jaime de la; Guzmán Lotero Edith Liliana

    2000-01-01

    Se realiza un estudio de Iguana iguana para establecer los estadios embrionarios que caracterizan la especie, bajo condiciones de incubaci6n artificial controladas de 32.5°C y humedad relativa promedio de 90% ± 2, colectándose y fijándose 5 huevos diarios a partir de la postura y durante 67 días. El peso promedio de los huevos fue de 15.4001 g, largo 3.84 mm, ancho 2.7 mm, notándose que estos son independientes del tiempo transcurrido de incubación. El peso de los embriones no presen...

  18. Un análisis de los determinantes del pago bajo Contratos de Préstamo Grupal

    OpenAIRE

    Santiago Pulido, Juan Manuel

    2013-01-01

    En la presente tesis doctoral se estudia la relación entre cohesión social y pago en contratos de préstamo grupal con las herramientas de la economía de redes sociales. En un primer planteamiento, el mecanismo del préstamo grupal se aproxima a uno de contribución voluntaria encontrando que el pago esperado de participar es función directa de la probabilidad de pago individual. Esta última se estudia en primer instancia bajo independencia en los eventos de pago. El mecanismo se tra...

  19. Humedad crítica y repelencia al agua en andisoles colombianos bajo cobertura de pinus patula schltdl y cham.

    OpenAIRE

    Jaramillo Jaramillo, Daniel Francisco

    2011-01-01

    En un lote con Andisoles bajo cobertura de Pinus patula Schltdl y Cham se ubicaron 4 transectos sobre los cuales se hizo un muestreo horizontal cada 5 cm, en 4 profundidades, con el fin de establecer si había una zona de transición de humedad crítica en el suelo que estuviera controlando la presencia de repelencia al agua en él. Se hicieron determinaciones de WDPT en el campo y luego en muestras secadas a 35°C, en laboratorio. En cada condición se determinó el contenido gravimétrico de hum...

  20. Caracterización de 14 genotipos de tomate (Lycopersicon esculentum Mill. cultivados bajo invernadero en Costa Rica

    Directory of Open Access Journals (Sweden)

    José Eladio Monge-Pérez

    2014-11-01

    Full Text Available Se caracterizaron 14 genotipos de tomate producidos bajo invernadero, tanto a nivel cualitativo como cuantitativo. Los datos muestran una amplia variabilidad entre genotipos y brindan información útil a los productores en el proceso de selección del genotipo a utilizar en su sistema productivo, según el nicho de mercado de interés. Se destacan varios genotipos de tomate tipo “cherry! y “uva” por su alto contenido de sólidos solubles, que pueden ser opciones para los consumidores que demandan un producto de alta calidad.

  1. Determinantes sociales de la mortalidad infantil en municipios de bajo índice de desarrollo humano en México

    OpenAIRE

    Duarte-Gómez, María Beatriz; Núñez-Urquiza, Rosa María; Restrepo-Restrepo, José Alonso; Richardson-López-Collada, Vesta Louise

    2015-01-01

    Introducción: Se realizó un estudio con el objetivo de identificar determinantes sociales de mortalidad infantil en zonas rurales en México, y recomendar estrategias para disminuir esta mortalidad. Métodos: Se tomó una muestra por conveniencia de 16 municipios de bajo índice de desarrollo humano. Se identificaron fallecimientos de menores de un año de edad a través de registros oficiales y de entrevistas con autoridades civiles, personal de salud y líderes comunitarios. También se realizar...

  2. Fortificaciones de la II Edad del Hierro en el Bajo Aragón : un estilo regional

    OpenAIRE

    Melguizo , Salvador; Moret , Pierre

    2006-01-01

    International audience; Se estudian en esta comunicación las fortificaciones del final de la época ibérica en dos áreas del Bajo Aragón: la desembocadura del Regallo, en Caspe, con los yacimientos de La Tallada y Palermo I, y el valle medio del Matarraña, entre Calaceite y Valdeltormo, con los yacimientos de San Antonio, Els Castellans y Torre Cremada. Se observa en ellos un patrón repetitivo, caracterizado por la presencia de tres elementos, concentrados en la zona de acceso más vulnerable: ...

  3. Desarrollo comunitario en el medio rural: un proyecto en la mancomunidad de Villas del Bajo Carrion y Ucieza

    OpenAIRE

    Giraldo Rojo, Cristina

    2017-01-01

    Este proyecto de fin de grado ha sido elaborado con el fin de hallar teorías de desarrollo comunitario, así como también realizar un análisis de estas mismas. De igual forma que se han analizado las iniciativas por parte de los diferentes agentes y administraciones, para después elaborar y planificar unas actividades de desarrollo comunitario en la Mancomunidad de Villas del bajo Carrión y Ucieza, situado en la provincia de Palencia. Grado en Educación Social

  4. Incidencia de la estrategia colombiana de desarrollo bajo en carbono en el mercado de bonos de carbono en Colombia

    OpenAIRE

    Castellanos Garcia, Camilo Andres

    2015-01-01

    La presente investigación tiene como objetivo principal determinar el papel de la estrategia colombiana de desarrollo bajo en carbono en el mercado de bonos de carbono en Colombia. Esto con el fin de demostrar que las acciones nacionalmente apropiadas de mitigación NAMAS son un mecanismo de mitigación y puede no tener una incidencia en la oferta ni tampoco en la demanda del mercado de bonos de carbono en Colombia. Esta investigación se llevará a cabo por medio de un estudio de caso con un enf...

  5. Diseño de un cuadricóptero de bajo coste basado en herramientas de software libre

    OpenAIRE

    Ciudad Gómez, Rubén

    2016-01-01

    El presente proyecto tiene como objetivo la realización de un cuadricóptero de bajo coste desarrollado con herramientas de software libre, con el fin de permitir el acceso y modificación del mismo a cualquiera que posea los conocimientos necesarios. Los cuadricópteros se definen como un vehículo aéreo no tripulado con cuatro rotores en los extremos. Los ejemplos existentes de estos tipos de vehículos son casi siempre de grandes cuadricópteros, los cuales utilizan diferentes tecnologías en ...

  6. Riesgos y consecuencias de las prácticas sexuales en adolescentes bajo los efectos de alcohol y otras drogas

    OpenAIRE

    Guillermo Castaño Pérez; Eduardo Arango Tobón; Santiago Morales Mesa; Alexander Rodríguez Bustamante; Carolina Montoya Montoya

    2013-01-01

    Introducción: la sexualidad, y el consumo de alcohol y drogas en los adolescentes, figuran en la actualidad como uno de los asuntos con mayor prioridad para la salud pública. La preocupación radica en reducir los riesgos de infecciones de transmisión sexual, el virus de la inmunodeficiencia humana y los embarazos tempranos no deseados. Objetivo: analizar las prácticas sexuales bajo el efecto de alcohol y otras drogas, y los riesgos que esto implica, en los adolescentes de la ciudad de Medellí...

  7. Desarrollo de alimentos tipo snack de bajo contenido graso a partir de discos de manzana verde

    OpenAIRE

    Tavera Quiroz, María José

    2015-01-01

    Esta investigación se basó en el desarrollo de productos tipo snack bajos en grasa a partir de manzana verde Granny Smith (Malus domestica). Se desarrollaron snacks a partir de manzanas verdes estudiando diferentes alternativas de cocción (fritado, horneado o su combinación) con el objetivo de obtener productos con menor contenido graso que los tradicionales pero con las propiedades que los consumidores valoran en los mismos, como el color y la textura crocante. Para ello se analizó...

  8. Estudios biológicos sobre procambarus clarkii girard (decapoda cambaridae) en las marismas del bajo Guadalquivir

    OpenAIRE

    Cano Sánchez, Esperanza

    1994-01-01

    Procambarus clarkii Girard, es un cangrejo de agua dulce, vulgarmente conocido como cangrejo americano o rojo, originario de Estados Unidos (zona Sur central) y México (zona Noreste) (HOBBS, 1972 a). Fue introducido en Las Marismas del Bajo Guadalquivir en 1974 y debido a que presenta una alta tasa de reproducción, actividad excavadora y buena adaptación a zonas con drásticas fluctuaciones temporales en el nivel del agua (HUNER & BARR, 1984 a), en poco tiempo se extendió por toda la marisma. ...

  9. Determinantes sociales de la mortalidad infantil en municipios de bajo índice de desarrollo humano en México

    OpenAIRE

    Duarte-Gómez, María Beatriz; Núñez-Urquiza, Rosa María; Restrepo-Restrepo, José Alonso; López-Collada, Vesta Louise Richardson

    2015-01-01

    ResumenINTRODUCCIÓN: Se realizó un estudio con el objetivo de identificar determinantes sociales de mortalidad infantil en zonas rurales en México, y recomendar estrategias para disminuir esta mortalidad.MÉTODOS: Se tomó una muestra por conveniencia de 16 municipios de bajo índice de desarrollo humano. Se identificaron fallecimientos de menores de un año de edad a través de registros oficiales y de entrevistas con autoridades civiles, personal de salud y líderes comunitarios. También se reali...

  10. Distribución de tensiones tangenciales en vigas de sección constante bajo esfuerzos cortantes

    OpenAIRE

    Fernández, T.; Viaño, J.; Samartín, A.

    2000-01-01

    En este trabajo se analizan algunos resultados obtenidos por Trabucho y Viaño, mediante desarrollos asintótico de las ecuaciones de la elasticidad tridimensional en vigas de sección constante, para la distribución de tensiones tangenciales en una sección transversal bajo la acción de un esfuerzo cortante. Se aplican, con carácter ilustrativo, a vigas con distintos tipos de secciones transversales (rectangular, circular, triangular y perfil UPN) comparando los resultados con los qu...

  11. Selección de portafolios usando simulación y optimización bajo incertidumbre

    Directory of Open Access Journals (Sweden)

    Claudia Lorena Martinez Torres

    2004-01-01

    Full Text Available Los métodos utilizados usualmente en la formación de portafolios, son basados en hipótesis que no son reales en mercados emergentes, y en consecuencia no son aplicables a estos mercados. Ellos no indican la forma en que debe ser conformado el portafolio. Se presenta entonces, una metodología basada en simulación y optimización bajo incertidumbre que permite realizar la conformación del portafolio. Se realizan pruebas en el mercado colombiano y la Bolsa de Nueva York.

  12. The preferential accumulation of cadmium in the head portion of the freshwater planarian, Dugesia japonica (Platyhelminthes: Turbellaria).

    Science.gov (United States)

    Wu, Jui-Pin; Chen, Hon-Cheng; Li, Mei-Hui

    2011-12-01

    Free-living freshwater planarians are considered to have the potential for development as an experimental model for toxicological studies on xenobiotics, including metals. However, little was known about the distribution patterns of metals in the body of treated planarians. This study was conducted to determine the tissue distribution patterns of cadmium (Cd) in different body portions of the treated planarian, Dugesia japonica. Results showed that Cd accumulated in the head of planarians at a significantly higher concentration than in the tail. After examining the level of metallothionein (MT), we suggested that the tissue distribution pattern of Cd might be related to MT induction patterns. In contrast, in planarians treated with copper (Cu), neither the tissue accumulation of Cu nor the multiples of induction of MTs significantly differed between different portions. Furthermore, a higher Cd accumulation rate in the head of planarians caused more-severe oxidative stress to appear in this portion and also a higher susceptibility to a lethal concentration of Cd. Finally, both in vitro and in vivo acetylcholinesterase activities in both body portions of planarians were inhibited by Cd. The present study provides the first report that different metals are distributed in various body portions with different patterns in the planarian.

  13. [Optimization of lime milk precipitation process of Lonicera Japonica aqueous extract based on quality by design concept].

    Science.gov (United States)

    Shen, Jin-Jing; Gong, Xing-Chu; Pan, Jian-Yang; Qu, Hai-Bin

    2017-03-01

    Design space approach was applied in this study to optimize the lime milk precipitation process of Lonicera Japonica (Jinyinhua) aqueous extract. The evaluation indices for this process were total organic acid purity and amounts of 6 organic acids obtained from per unit mass of medicinal materials. Four critical process parameters (CPPs) including drop speed of lime milk, pH value after adding lime milk, settling time and settling temperature were identified by using the weighted standardized partial regression coefficient method. Quantitative models between process evaluation indices and CPPs were established by a stepwise regression analysis. A design space was calculated by a Monte-Carlo simulation method, and then verified. The verification test results showed that the operation within the design space can guarantee the stability of the lime milk precipitation process. The recommended normal operation space is as follows: drop speed of lime milk of 1.00-1.25 mL•min⁻¹, pH value of 11.5-11.7, settling time of 1.0-1.2 h, and settling temperature of 10-20 ℃.. Copyright© by the Chinese Pharmaceutical Association.

  14. Effect of Cold-Water Irrigation on Grain Quality Traits in japonica Rice Varieties from Yunnan Province, China

    Directory of Open Access Journals (Sweden)

    Guo-zhen ZHAO

    2009-09-01

    Full Text Available The response of grain quality traits to cold-water irrigation and its correlation with cold tolerance were studied in 11 japonica rice varieties from Yunnan Province, China. The results indicated that the response of grain quality traits to the cold-water stress varied with rice varieties and grain quality traits. Under the cold-water stress, grain width, chalky rice rate, whiteness, 1000-grain weight, brown rice rate, taste meter value, peak viscosity, trough viscosity, breakdown viscosity and final viscosity significantly decreased, whereas grain length-width ratio, head rice rate, alkali digestion value, protein content and setback viscosity markedly increased. However, the other traits such as grain length, amylose content, milled rice rate, peak viscosity time and pasting temperature were not significantly affected by the cold-water stress. Significant correlations were discovered between phenotypic acceptability and cold response indices of taste meter value, protein content, peak viscosity and breakdown viscosity. Therefore, it would be very important to improve the cold tolerance of Yunnan rice varieties in order to stabilize and improve their eating quality.

  15. Characterization of lecithin isolated from anchovy (Engraulis japonica) residues deoiled by supercritical carbon dioxide and organic solvent extraction.

    Science.gov (United States)

    Lee, Seung-Mi; Asaduzzaman, A K M; Chun, Byung-Soo

    2012-07-01

    Lecithin was isolated and characterized from anchovy (Engraulis japonica) deoiled residues using supercritical carbon dioxide (SC-CO(2)) at a semibatch flow extraction process and an organic solvent (hexane) extraction. SC-CO(2) extraction was carried out to extract oil from anchovy at different temperatures (35 to 45 °C) and pressures (15 to 25 MPa). Extraction yield of oil was influenced by physical properties of SC-CO(2) with temperature and pressure changes. The major phospholipids of anchovy lecithin were quantitatively analyzed by high-performance liquid chromatography. Phosphatidylcholine (PC) (68%± 1.00%) and phosphatidylethanolamine (PE) (29%± 0.50%) were the main phospholipids. Thin layer chromatography was performed to purify the individual phospholipids. The fatty acid compositions of lecithin, PC, and PE were analyzed by gas chromatography. A significant amount of eicosapentaenoic acid and docosahexaenoic acid were present in both phospholipids of PC and PE. Emulsions of lecithin in water were prepared through the use of a homogenizer. Oxidative stability of anchovy lecithin was high in spite of its high concentration of long-chain polyunsaturated fatty acids. Lecithin can be totally metabolized by humans, so is well tolerated by humans and nontoxic when ingested. Lecithin from anchovy contain higher amounts of ω-3 fatty acids especially EPA and DHA, it may have positive outcome to use in food and pharmaceutical industries. © 2012 Institute of Food Technologists®

  16. Effects of dietary nucleotides on growth, non-specific immune response and disease resistance of sea cucumber Apostichopus japonicas.

    Science.gov (United States)

    Wei, Zehong; Yi, Lina; Xu, Wei; Zhou, Huihui; Zhang, Yanjiao; Zhang, Wenbing; Mai, Kangsen

    2015-11-01

    A 9-week feeding trial was conducted to investigate the effects of dietary nucleotides (NT) on growth, immune response and disease resistance of sea cucumber Apostichopus japonicas (initial weight: 5.87 ± 0.03 g). Four graded levels of dietary NT were designed as 0, 150, 375 and 700 mg/kg, respectively. After the feeding trial, sea cucumbers were challenged with Vibrio splendidus for the determination of disease resistance. The results showed that the specific growth rates were significantly higher in sea cucumber fed the diet with 375 mg/kg NT than those fed the basal diet without NT supplementation (P sea cucumber fed diets with nucleotides (≥ 375 mg/kg) had significantly higher phagocytic activities in coelomic fluid (P 0.05). After being challenged with V. splendidus, the cumulative mortalities of sea cucumber fed diets with 150 and 375 mg/kg NT were significantly lower than that in the treatment without dietary nucleotide supplementation (P sea cucumber in vivo. In conclusion, it was showed that dietary NT does increase the growth performance, non-specific immunity and disease resistance of sea cucumber. The optimum dietary NT supplementation level for sea cucumber was found to be 375 mg/kg. The application of dietary NT may present a novel strategy for health management in sea cucumber's aquaculture. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Evaluation of the Photoprotective Effect of Dongchongxiacao (Paecilomyces japonica) Extract against Ultraviolet Radiation-induced Skin Wrinkling and Cancer

    International Nuclear Information System (INIS)

    Lee, Hae June; Moon, Chang Jong; Kim, Jong Choon; Kim Sung Ho; Jung, Uhee; Jo, Sung Kee; Jang, Jong Sik

    2012-01-01

    To evaluate the ability of Dongchongxiacao (Paecilomyces japonica ) extract (PJE) to protect the skin from photo damage, the gross and microscopic changes in the skin of hairless mice and PJE-treated mice exposed chronically to ultraviolet (UV) were examined. The skin of the UV-irradiated mice showed characteristic signs of photo aging, such as deep wrinkles across the back. PJE-treated mice showed a significantly decreased wrinkling score. By the 22nd week, 88.9% (i.p. with saline) or 44.4% (topical administration with cream base) of the UV-irradiated mice developed at least one tumor. PJE delayed tumor onset significantly. PJE (i.p.) was also effective in reducing the occurrence of UV radiation-induced skin tumors and reduced the number of tumors per mouse. After 22 weeks of treatment, 80.0% (i.p.) and 75.0% (topical) of the mice treated with PJE were tumor-free. Tumor multiplicity was reduced by 96.2% (i.p.) in the PJE treated groups. It is noted that skin that is chronically exposed to UV is subject to photo aging and photo carcinogenesis and regular use of PJE would prevent these photo damaging effects of UV.

  18. Biometric-based estimation of net ecosystem production in a mature Japanese cedar (Cryptomeria japonica) plantation beneath a flux tower.

    Science.gov (United States)

    Yashiro, Yuichiro; Lee, Na-Yeon M; Ohtsuka, Toshiyuki; Shizu, Yoko; Saitoh, Taku M; Koizumi, Hiroshi

    2010-07-01

    Quantification of carbon budgets and cycling in Japanese cedar (Cryptomeria japonica D. Don) plantations is essential for understanding forest functions in Japan because these plantations occupy about 20% of the total forested area. We conducted a biometric estimate of net ecosystem production (NEP) in a mature Japanese cedar plantation beneath a flux tower over a 4-year period. Net primary production (NPP) was 7.9 Mg C ha(-1) year(-1) and consisted mainly of tree biomass increment and aboveground litter production. Respiration was calculated as 6.8 (soil) and 3.3 (root) Mg C ha(-1) year(-1). Thus, NEP in the plantation was 4.3 Mg C ha(-1) year(-1). In agreement with the tower-based flux findings, this result suggests that the Japanese cedar plantation was a strong carbon sink. The biometric-based NEP was higher among most other types of Japanese forests studied. Carbon sequestration in the mature plantation was characterized by a larger increment in tree biomass and lower mortality than in natural forests. Land-use change from natural forest to Japanese cedar plantation might, therefore, stimulate carbon sequestration and change the carbon allocation of NPP from an increment in coarse woody debris to an increase in tree biomass.

  19. Degradation Dynamics and Dietary Risk Assessments of Two Neonicotinoid Insecticides during Lonicera japonica Planting, Drying, and Tea Brewing Processes.

    Science.gov (United States)

    Fang, Qingkui; Shi, Yanhong; Cao, Haiqun; Tong, Zhou; Xiao, Jinjing; Liao, Min; Wu, Xiangwei; Hua, Rimao

    2017-03-01

    The degradation dynamics and dietary risk assessments of thiamethoxam and thiacloprid during Lonicera japonica planting, drying, and tea brewing processes were systematically investigated using high-performance liquid chromatography. The half-lives of thiamethoxam and thiacloprid were 1.0-4.1 d in the honeysuckle flowers and leaves, with degradation rate constants k ranging from -0.169 to -0.696. The safety interval time was 7 d. The sun- and oven-drying (70 °C) percent digestions were 59.4-81.0% for the residues, which were higher than the shade- and oven-drying percentages at lower temperatures (30, 40, 50, and 60 °C, which ranged from 37.7% to 57.0%). The percent transfers of thiamethoxam and thiacloprid were 0-48.4% and 0-25.2%, respectively, for the different tea brewing conditions. On the basis of the results of this study, abiding by the safety interval time is important, and using reasonable drying methods and tea brewing conditions can reduce the transfer of thiamethoxam and thiacloprid to humans.

  20. Studies on cytological, physiological and genetic characteristics in somatic mutant strains of Sugi (Cryptomeria japonica D. Don)

    International Nuclear Information System (INIS)

    Maeta, T.; Somegou, M.; Nakahira, K.; Miyazaki, Y.; Kondo, T.

    1982-01-01

    From microscopic observation of the pollen of induced mutant strains in Sugi (Cryptomeria japonica D. Don), it was found that there were large differences in pollen fertility among the mutant strains, and that it deviated year to year from the mother plants. The large differences in frequency of sterile pollen among mutant strains depended on the genetic characteristics of each mutant strain. Higher frequencies of sterile pollen were observed at the terminal part of branchlets in some mutant strains, and this was considered to be induced by the lateness of flower-bud formation at low temperature conditions in late summer. Delayed formation and gibberellic acid treatment applied for flower induction resulted in low fertility and abnormality of pollen in mutant strains. Chromosome aberration in mutant strains was caused either by gamma irradiation or by some mutational events that responded to environmental conditions. In the former case, aberration might have been maintained for a long period through vegetative propagation. Some of the irregularities were due to mitotic cell division, because cells with micronuclei at the pacytene stage in pollen mother cells and with fragments at MI were observed. Somatic mutability of Kuma-sugi mutants after re-irradiation was investigated. From waxless mutants morphological somatic mutations, which have fat or stout stems and thick and short needles, were frequently produced, whereas from morphological mutants the lowest somatic mutation frequency was induced. In some mutant strains higher rooting ability than the mother plants was found, and the possibility of character improvement was pointed out. (author)

  1. Comparative Study of the Phytoprostane and Phytofuran Content of indica and japonica Rice (Oryza sativa L.) Flours.

    Science.gov (United States)

    Pinciroli, M; Domínguez-Perles, R; Abellán, A; Guy, A; Durand, T; Oger, C; Galano, J M; Ferreres, F; Gil-Izquierdo, A

    2017-10-11

    Phytoprostanes and phytofurans (PhytoPs and PhytoFs, respectively) are nonenzymatic lipid peroxidation products derived from α-linolenic acid (C18:3 n-3), considered biomarkers of oxidative degradation in plant foods. The present work profiled these compounds in white and brown grain flours and rice bran from 14 rice cultivars of the subspecies indica and japonica by ultrahigh performance liquid chromatography coupled to electrospray ionization and triple quadrupole mass spectrometry. For PhytoPs, the average concentrations were higher in rice bran (0.01-9.35 ng g -1 ) than in white and brown grain flours (0.01-1.17 ng g -1 ). In addition, the evaluation of rice flours for the occurrence PhytoFs evidenced average values 1.77, 4.22, and 10.30 ng g -1 dw in rice bran, brown grain flour, and white grain flour, respectively. A significant correlation was observed between total and individual compounds. The concentrations retrieved suggest rice bran as a valuable source of PhytoPs and PhytoFs that should be considered in further studies on bioavailability and bioactivity of such compounds.

  2. Comparison of Essential Oils from Three Kinds of Cryptotaenia japonica Hassk (Kirimitsuba, Nemitsuba, and Itomitsuba) used in Japanese Food.

    Science.gov (United States)

    Okuno, Yoshiharu; Marumoto, Shinsuke; Miyazawa, Mitsuo

    2017-11-01

    The compositions of the essential oils from three kinds of Cryptotaenia japonica Hassk ("Mitsuba" in Japanese, Kirimitsuba (KM), Nemitsuba (NM), and Itomitsuba (IM)) were investigated by capillary GC/GC-MS. The oils contained 53 volatile components, of which 95% were terpenoids. The major constituents were sesquiterpenoids, which were α-selinene (KM: 39.1%; NM: 38.4%; IM: 13.2%), β-selinene (15.5%, 15.2%, 4.8%), germacrene D (12.1%, 7.2%, 24.1%), trans-farnesene (11.1%, 6.0%, 10.9%), β-elemene (2.9%, 2.9%, 6.8%), and trans-caryophyllene (1.7%, 1.7%, 2.6%). The main sesquiterpene found in KM and NM was α-selinene and in IM was germacrene D. The major monoterpenes found were β-myrcene (3.8%, 6.7%, and 3.5%) and β-pinene (2.8%, 0.2%, and 1.4%).

  3. Effect of Calcium Chloride on the Permeation of the Cryoprotectant Dimethyl Sulfoxide to Japanese Whiting Sillago japonica Embryos

    Science.gov (United States)

    Rahman, Sk. Mustafizur; Majhi, Sullip Kumar; Suzuki, Toru; Strussmann, Carlos Augusto; Watanabe, Manabu

    Cryopreservation of fish eggs and embryos is a highly desired tool to promote aquaculture production and fisheries resource management, but it is still not technically feasible. The failure to develop successful cryopreservation protocols for fish embryos is largely attributed to poor cryoprotectant permeability. The purpose of this study was to test the effectiveness of CaCl2 to enhance cryoprotectant uptake by fish embryos. In this study, embryos (somites and tail elongation stages) of Japanese whiting Sillago japonica were exposed to 10 and 15% dimethyl sulfoxide (DMSO) in artificial sea water (ASW) or a solution of 0.125M CaCl2 in distilled water for 20 min at 24°C. The toxicity of all solutions was estimated from the hatching rates of the embryos and High Performance Liquid Chromatography was used to determine the amount of DMSO taken up during impregnation. The results showed that DMSO incorporation into the embryos was greatly (›50%) enhanced in the presence of CaCl2 compared to ASW. CaCl2 itself was not toxic to the embryos but, probably as a result of the enhanced DMSO uptake, caused decreases in survival of about 14-44% relative to ASW. Somites stage embryos were more tolerant than tail elongation ones to DMSO both as ASW and CaCl2 solutions. The use of CaCl2 as a vehicle for DMSO impregnation could be a promising aid for the successful cryopreservation of fish embryos.

  4. Symbiotic association of Photobacterium fischeri with the marine luminous fish Monocentris japonica; a model of symbiosis based on bacterial studies.

    Science.gov (United States)

    Ruby, E G; Nealson, K H

    1976-12-01

    Isolation of bacteria from the luminous organ of the fish Monocentris japonica has revealed that the organ contains a pure culture of luminous bacteria. For the four fish examined, all contained Photobacterium fischeri as their luminous bacterial symbiont. This is the first time that P. fischeri has been identified in a symbiotic association. A representative isolate (MJl) of the light organ population was selected for in vivo studies of its luminous system. Several physiological features suggest adaptation for symbiotic existence. First, MJl has been shown to produce and respond to an inducer of luciferase that could accumulate in the light organ. Secondly, the specific activity of light production was seen to be maximal under low, growth-limiting concentrations of oxygen. Thirdly, unlike another luminous species (Beneckea harveyi), synthesis of the light production system of these bacteria is not catabolite repressed by glucose--a possible source of nutrition in the light organ. Fourthly, when grown aerobically on glucose these bacteria excrete pyruvic acid into the medium. This production of pyruvate is a major process, accounting for 30-40% of the glucose utilized and may serve as a form of regulatory and nutritional communication with the host.

  5. Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

    Directory of Open Access Journals (Sweden)

    Yong Joon Jeong

    Full Text Available Osteoarthritis (OA is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH extract of Litsea japonica fruit (LJFE had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

  6. Mechanism study of endothelial protection and inhibits platelet activation of low molecular weight fucoidan from Laminaria japonica

    Science.gov (United States)

    Chen, Anjin; Zhang, Fang; Shi, Jie; Zhao, Xue; Yan, Meixing

    2016-10-01

    Several studies have indicated that fucoidan fractions with low molecular weight and different sulfate content from Laminaria japonica could inhibit the activation of platelets directly by reducing the platelet aggregation. To explore the direct effect of LMW fucoidan on the platelet system furthermore and examine the possible mechanism, the endothelial protection and inhibits platelet activation effects of two LMW fucoidan were investigated. In the present study, Endothelial injury model of rats was made by injection of adrenaline (0.4 mg kg-1) and human umbilical vein endothelial cells were cultured. vWF level was be investigated in vivo and in vitro as an important index of endothelial injury. LMW fucoidan could significantly reduce vWF level in vascular endothelial injury rats and also significantly reduce vWF level in vitro. The number of EMPs was be detected as another important index of endothelial injury. The results showed that LMW fucoidan reduced EMPs stimulated by tumor necrosis factor. In this study, it was found that by inhibiting platelet adhesion, LMW fucoidan played a role in anti-thrombosis and the specific mechanism of action is to inhibit the flow of extracellular Ca2+. All in a word, LMW fucoidan could inhibit the activation of platelets indirectly by reducing the concentration of EMPs and vWF, at the same time; LMW fucoidan inhibited the activation of platelets directly by inhibiting the flow of extracellular Ca2+.

  7. Characteristics and genetic mapping of a lesion mimic mutant pl(t) in japonica rice variety zhejing 22

    International Nuclear Information System (INIS)

    Chen Pingping; Zhang Xiaoming; Ye Shenghai; Zhao Ningchun; Lu Yanting; Liu Heqin; Jin Qingsheng; Yang Ling

    2010-01-01

    A lesion mimic mutant,obtained by radiation mutagenesis on the seeds of a japonica rice variety Zhejing 22, exhibited a lesion mimic phenotype during the whole growth stage under different environments. Genetic analysis indicated that the mutant trait was controlled by a single recessive gene named spl (t). Relying on simple sequence repeat (SSR) and recessive class analysis method to map the spl (t) gene with a F 2 population was constructed by crossing the mutant spl (t) with Zhenshan 97B.spl (t) was mapped in the interval of 0.8cM between RM7195 and RM27929 near centromere region on the short arm of chromosome 12.Blue trypan dye analyses indicated that the lesion mimic trait of the mutant was caused by the programmer cell death. Further study showed that the programmer cell death was caused by H 2 O 2 oxidative burst. By inoculation of bacterial leaf blight and blast strains, the resistances of the mutant were similar to the wild variety Zhejing 22. (authors)

  8. Optimization of the microwave-assisted extraction of phlorotannins from Saccharina japonica Aresch and evaluation of the inhibitory effects of phlorotannin-containing extracts on HepG2 cancer cells

    Science.gov (United States)

    He, Zhizhou; Chen, Yongshun; Chen, Yongheng; Liu, Haohuai; Yuan, Guanfu; Fan, Yaming; Chen, Kun

    2013-09-01

    The use of a microwave-assisted extraction (MAE) method for the extraction of phlorotannins from Saccharina japonica Aresch ( S. japonica) has been evaluated with particular emphasis on the influential parameters, including the ethanol concentration, solid/liquid ratio, extraction time, extraction temperature, and microwave power. The MAE procedure was optimized using single-factor design and orthogonal array design (OAD). The content of total phlorotannins in S. japonica was determined using a Folin-Ciocalteu (FC) assay. A maximum total phlorotannin content of 0.644 mg of phloroglucinol equivalent per gram of dry weight plant (mg PGE/g DW) was obtained using the optimized model, which included an ethanol concentration of 55%, solid/liquid ratio of 1:8, extraction time of 25 min, irradiation power of 400 W, and temperature of 60°C. Under similar conditions, the application of a conventional extraction method led to a lower phlorotannin yield of 0.585 mg PGE/g WD. These results demonstrated that the MAE approach provided better results for the extraction of phlorotannins from S. japonica and was a promising technique for the extraction of phenolic compounds from S. japonica and other materials. In addition, screening tests for the inhibitory activity showed that the phlorotannin-containing extracts significantly inhibited the growth of human hepatocellular carcinoma cells (HepG2) by inducing their apoptosis. The morphological changes that occurred during cell apoptosis were characterized using Hoechst33258 staining.

  9. Population and economy in the Bajo Ebro in Roman times. Basic concepts for a rural landscape model | Poblamiento y economía en el Bajo Ebro en época romana. Bases para un modelo de paisaje rural

    Directory of Open Access Journals (Sweden)

    Víctor Revilla Calvo

    1994-12-01

    Full Text Available This paper analyses the settlement patterns and the social-economic structures in Bajo Ebro in Roman times. It covers especifically relationships among settlements, rural landscape, structures of agricultural production and landowning. Likewise, the origin and historical evolution of these structures are studied, as well as their role within a broader political, social and economic framework in the Province of Tarraconensis. | El artículo analiza las formas del poblamiento y las estructuras socio-económicas del Bajo Ebro en época romana. Se plantean, en particular, las relaciones entre la distribución del habitat, el paisaje rural, las estructuras de la producción agrícola y de la propiedad. Se analiza igualmente el origen y evolución histórica de estas estructuras, así como su integración en un marco político, social y económico más amplio, constituido por la provincia Tarraconense.

  10. LOCAL GENIUS AS SOCIO-CULTURAL CAPITAL FOR EMPOWERING THE BAJO ETHNIC PEOPLE RESIDING AT THE COASTAL AREA OF BUNGIN PERMAI VILLAGE, SOUTH EAST SULAWESI

    Directory of Open Access Journals (Sweden)

    La Ode Ali Basri

    2012-11-01

    Full Text Available In this dissertation the local genius as socio-cultural capital for empowering theBajo ethnic people residing at the costal area of Bungin Permai Village, TinanggeaDistrict, South Konawe Regency, South Sulawesi Province is discussed. The Bajo ethnicpeople have a set of local genius within their socio-cultural system which is reflected intheir belief, tradition and custom and is used as the reference for conceiving andexplaining the objective and essence of life and the world. However, such local geniushas not functioned optimally yet as they are still marginalized.This research is focused on (1 what forms of local genius serve as the sociocultural capital for empowering the Bajo ethnic group residing at the coastal area?; (2how the local genius is developed to empower the Bajo ethnic people residing at thecoastal area?; and (3 what factors which may support and obstruct the local genius usedas the socio cultural capital for empowering the Bajo ethnic people residing at the coastalarea? Qualitative method is employed in this study with the approach of cultural studies.The theories used are the post colonial theory, structural theory, generative theory,hegemony theory and semiotic theory. The techniques used for collecting the data neededare participative observation, in-depth interview, library research, and focus groupdiscussion. The data obtained are analytically and descriptively processed and arepresented in the forms of narration, tables and visual illustration.The results of the study show that the Bajo ethnic people residing at BunginPermai Village have a set of local genius which may be potentially used as the sociocultural capital for empowering their community such as (1 indigenous skills andknowledge; (2 working culture; and (3 local organizations. The development of theindigenous skills and knowledge (pengetahuan dan ketrampilan asli; hereon abbreviatedto PKA and the revitalization of their local organizations may be used as the

  11. Análisis multifactorial de los factores de riesgo de bajo peso al nacer en Salvador, Bahia

    Directory of Open Access Journals (Sweden)

    Solla Jorge José Santos Pereira

    1997-01-01

    Full Text Available El presente estudio constituye un análisis multifactorial de los factores de riesgo de bajo peso al nacer en un grupo de recién nacidos en una zona urbana del Brasil. Se incluyeron en el estudio un total de 1 023 nacidos vivos, dados a luz en cuatro maternidades de Salvador, Bahia, entre julio de 1987 y febrero de 1988. Las fuentes de información fueron las historias clínicas y las entrevistas con las madres en la maternidad. El análisis se realizó mediante regresión logística. En el modelo final los factores de riesgo incluidos fueron los siguientes: edad materna menos de 21 años o más de 35; edad gestacional menos de 38 semanas; resultado desfavorable del embarazo anterior; intervalo intergenésico previo de 12 meses o menos; tabaquismo; e hipertensión. Se presentan los valores del riesgo atribuible poblacional para los factores de riesgo incluidos en el modelo final. Esos factores deben emplearse para detectar a las gestantes con alto riesgo de dar a luz un niño de bajo peso, a las que debe brindarse mayor atención prenatal.

  12. Bajo peso al nacer y embarazo en la adolescencia en cinco consultorios del médico de la familia

    Directory of Open Access Journals (Sweden)

    Ramón Pérez Queda

    1995-06-01

    Full Text Available Se realizó un estudio de 120 embarazadas en un área de 5 consultorios del médico de la familia en un período que comprendió de enero de 1987 a enero de 1990; de ellas, 56 eran menores de 20 años para el 46,6 %. Se realizó un estudio comparativo entre éstas y las gestantes de más de 20 años de edad, y se observó que las adolescentes presentaron mayor número de recién nacidos con bajo peso (88 % y partos pretérminos. El consumo del tabaco y la desnutrición materna fueron factores importantes que se añadieron a la adolescencia en el resultado del bajo peso al nacer.A study was performed on 120 pregnant women in an area of 5 medical offices of the Family Doctor plan from January 1987 to January 1990. Of them 56 were under 20 years of age accounting for 46,6 %. A comparative study was carried out between this group and pregnant women over 20 years, and it was observed that teenagers had a greater number of low birth weight infants (88 % and preterm deliveries. Smoking and maternal malnutrition were considered as very important factors in addition to adolescence for low birth weight.

  13. Estudio del comportamiento de la escayola reforzada con fibras de sisal, para componentes en viviendas de bajo coste

    Directory of Open Access Journals (Sweden)

    de Oteiza San José, Ignacio

    1993-08-01

    Full Text Available The present article is a brief description of the work done for the elaboration of the doctoral thesis and its conclusions. Starting from the possibilities of using plaster in developing countries, due to its abundance and low cost, a series of mechanical tests have been carried out in order to learn about the physical and mechanical properties of plaster reinforced with sisal fibres for its future use in components for low cost housing.

    El presente artículo es una breve descripción de los trabajos realizados para la elaboración de la tesis doctoral y de las conclusiones de la misma. Partiendo de las posibilidades de utilización en los países en vías de desarrollo del material de escayola, por su abundancia y bajo coste, se llevan a cabo una serie de ensayos mecánicos, con el fin de conocer las propiedades físicas y mecánicas de la escayola reforzada con fibras de sisal, para una futura aplicación en componentes para viviendas de bajo coste.

  14. Comparison of chemical compositions and antimicrobial activities of essential oils from three conifer trees; Pinus densiflora, Cryptomeria japonica, and Chamaecyparis obtusa.

    Science.gov (United States)

    Lee, Jeong-Ho; Lee, Byung-Kyu; Kim, Jong-Hee; Lee, Sang Hee; Hong, Soon-Kwang

    2009-04-01

    The chemical compositions, and antibacterial and antifungal effects of essential oils extracted from three coniferous species, Pinus densiflora, Cryptomeria japonica, and Chamaecyparis obtusa, were investigated. Gas chromatography mass analysis of the essential oils revealed that the major components and the percentage of each essential oil were 16.66% beta-phellandrene and 14.85% alpha-pinene in P. densiflora; 31.45% kaur-16-ene and 11.06% sabinene in C. japonica; and 18.75% bicyclo [2, 2, 1] heptan-2-ol and 17.41% 2-carene in Ch. obtusa. The antimicrobial assay by agar disc diffusion method showed that 2.2 microg of Ch. obtusa oil inhibited most effectively the growth of Escherichia coli ATCC 33312 and Klebsiella oxytoca ATCC 10031, whereas the C. japonica oil gave weak antimicrobial activity. The minimal inhibitory concentration (MIC) values for bacterial strains were in the range of 5.45-21.8 mg/ml depending on essential oils, but most Gram-negative bacteria were resistant even at 21.8 mg oil/ml. P. densiflora oil showed the most effective antifungal activity and the MIC values for Cryptococcus neoformans B42419 and Candida glabrata YFCC 062CCM 11658 were as low as 0.545 and 2.18 mg/ml, respectively. Cryp. neoformans B42419 was the most sensitive to all essential oils in the range of 0.545-2.18 mg/ml. Our data clearly showed that the essential oils from the three conifers had effective antimicrobial activity, especially against fungi.

  15. Effects of ultrahigh pressure extraction on yield and antioxidant activity of chlorogenic acid and cynaroside extracted from flower buds of Lonicera japonica.

    Science.gov (United States)

    Hu, Wen; Guo, Ting; Jiang, Wen-Jun; Dong, Guang-Li; Chen, Da-Wei; Yang, Shi-Lin; Li, He-Ran

    2015-06-01

    The present study was designed to establish and optimize a new method for extracting chlorogenic acid and cynaroside from Lonicera japonica Thunb. through orthogonal experimental designl. A new ultrahigh pressure extraction (UPE) technology was applied to extract chlorogenic acid and cynaroside from L. japonica. The influential factors, including solvent type, ethanol concentration, extraction pressure, time, and temperature, and the solid/liquid ratio, have been studied to optimize the extraction process. The optimal conditions for the UPE were developed by quantitative analysis of the extraction products by HPLC-DAD in comparison with standard samples. In addition, the microstructures of the medicinal materials before and after extraction were studied by scanning electron microscopy (SEM). Furthermore, the extraction efficiency of different extraction methods and the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities of the extracts were investigated. The optimal conditions for extracting chlorogenic acid and cynaroside were as follows: ethanol concentration, 60%; extraction pressure, 400 MPa; extraction time, 2 min; extraction temperature, 30 °C; and the solid/liquid ratio, 1 : 50. Under these conditions, the yields of chlorogenic acid and cynaroside were raised to 4.863% and 0.080%, respectively. Compared with other extraction methods, such as heat reflux extraction (HRE), ultrasonic extraction (UE), and Sohxlet extraction (SE), the UPE method showed several advantages, including higher extraction yield, shorter extraction time, lower energy consumption, and higher purity of the extracts. This study could help better utilize L. japonica flower buds as a readily accessible source of natural antioxidants in food and pharmaceutical industries. Copyright © 2015 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  16. [Research on modeling method to analyze Lonicerae Japonicae Flos extraction process with online MEMS-NIR based on two types of error detection theory].

    Science.gov (United States)

    Du, Chen-Zhao; Wu, Zhi-Sheng; Zhao, Na; Zhou, Zheng; Shi, Xin-Yuan; Qiao, Yan-Jiang

    2016-10-01

    To establish a rapid quantitative analysis method for online monitoring of chlorogenic acid in aqueous solution of Lonicera Japonica Flos extraction by using micro-electromechanical near infrared spectroscopy (MEMS-NIR). High performance liquid chromatography(HPLC) was used as reference method.Kennard-Stone (K-S) algorithm was used to divide sample sets, and partial least square(PLS) regression was adopted to establish the multivariate analysis model between the HPLC analysis contents and NIR spectra. The synergy interval partial least squares (SiPLS) was used to selected modeling waveband to establish PLS models. RPD was used to evaluate the prediction performance of the models. MDLs was calculated based on two types of error detection theory, on-line analytical modeling approach of Lonicera Japonica Flos extraction process was expressed scientifically by MDL. The result shows that the model established by multiplicative scatter correction(MSC) was the best, with the root mean square with cross validation(RMSECV), root mean square error of correction(RMSEC) and root mean square error of prediction(RMSEP) of chlorogenic acid as 1.707, 1.489, 2.362, respectively, the determination coefficient of the calibration model was 0.998 5, and the determination coefficient of the prediction was 0.988 1.The value of RPD is 9.468.The MDL (0.042 15 g•L⁻¹) selected by SiPLS is less than the original,which demonstrated that SiPLS was beneficial to improve the prediction performance of the model. In this study, a more accurate expression of the prediction performance of the model from the two types of error detection theory, to further illustrate MEMS-NIR spectroscopy can be used for on-line monitoring of Lonicera Japonica Flos extraction process. Copyright© by the Chinese Pharmaceutical Association.

  17. Biochars derived from wasted marine macro-algae (Saccharina japonica and Sargassum fusiforme) and their potential for heavy metal removal in aqueous solution.

    Science.gov (United States)

    Poo, Kyung-Min; Son, Eun-Bi; Chang, Jae-Soo; Ren, Xianghao; Choi, Yun-Jung; Chae, Kyu-Jung

    2018-01-15

    For the purpose of reusing wasted marine macro-algae generated during cultivation, harvesting, processing and selling processes, biochars derived from Saccharina japonica (known as kelp) and Sargassum fusiforme (known as hijikia) were characterized and their removal capacities for Cu, Cd, and Zn in aqueous solution were assessed. Feedstocks, S. japonica, S. fusiforme, and also pinewood sawdust as a control, were pyrolyzed at 250, 400, 500, 600 and 700 °C. In evaluating heavy metal removal capacities, SJB (S. japonica biochar) showed the best performance, with removal efficiencies of more than 98% for the three heavy metals when pyrolyzed at over 400 °C. SFB (S. fusiforme biochar) also showed good potential as an adsorbent, with removal efficiencies for the three heavy metals of more than 86% when pyrolyzed at over 500 °C. On the contrary, the maximum removal efficiencies of PSB (pinewood sawdust biochar) were 81%, 46%, and 47% for Cu, Cd, and Zn, respectively, even at 700 °C, the highest pyrolysis temperature. This demonstrates that marine macro-algae were advantageous in terms of production energy for removing heavy metals even at relatively low pyrolysis temperatures, compared with PSB. The excellent heavy metal adsorption capacities of marine macro-algae biochars were considered due to their higher pH and more oxygen-containing functional groups, although the specific surface areas of SJB and SFB were significantly lower than that of PSB. This research confirmed that the use of marine macro-algae as a heavy metal adsorbent was suitable not only in the removal of heavy metals, but also in terms of resource recycling and energy efficiency. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Morphology and stucture of wild apple (Malus silvestris Mill..common pear (Pyrus cofnmunis L. and Chaenomeles japonica (Thunb Lindl. seeds

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    Stanisław Pelc

    2014-01-01

    Full Text Available The outer and inner structure of wild apple (Malus silvestris Mill., common pear (Pyrus communis L. and Chaenomeles japonica (Thunb. Lindl. seeds was investigated. It was found that the outer structure exhibits good diagnostic features expressed in the first place in the relief of the seed coat and further in the arrangement and appearance of the site of attachment of the free end of the funiculus and the shape of the seeds. In ripe seeds there is, under the thick seed coat, an endosperm layer completely surrounding the embryo which has large cotyledons and a thick rootlet.

  19. Foliar application of molybdenum reduces yield loss and pre-harvest sprouting in japonica rice seed subjected to simulated flooding during seed development and maturation

    OpenAIRE

    Tejakhod, Sujittra; Hammond, John P.; Ellis, Richard H.

    2018-01-01

    Flooding damages rice crops and its incidence is increasing. Foliar spray applications of molybdenum (100, 600 or 3000 mg Mo L-1), abscisic acid (ABA, 50 μM), or deionised water (control) were made to pot-grown plants of the Japonica rice cv. Gleva at flag leaf appearance to determine their effects on seed yield and pre-harvest sprouting after flooding. Plants were submerged , to simulate flooding, for four days from 20 or 30 days after anthesis (DAA). Seed yield per plant, seed weight, and p...

  20. Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1.

    Science.gov (United States)

    Miyashita, Yurina; Ohmae, Eiji; Ikura, Teikichi; Nakasone, Kaoru; Katayanagi, Katsuo

    2017-05-01

    Dihydrofolate (DHF) reductase coded by a plasmid of the extremely halophilic archaeon Haloarcula japonica strain TR-1 (HjDHFR P1) shows moderate halophilicity on enzymatic activity at pH 6.0, although there is no significant effect of NaCl on its secondary structure. To elucidate the salt-activation and -inactivation mechanisms of this enzyme, we investigated the effects of pH and salt concentration, deuterium isotope effect, steady-state kinetics, and rapid-phase ligand-binding kinetics. Enzyme activity was increased eightfold by the addition of 500 mM NaCl at pH 6.0, fourfold by 250 mM at pH 8.0, and became independent of salt concentration at pH 10.0. Full isotope effects observed at pH 10.0 under 0-1000 mM NaCl indicated that the rate of hydride transfer, which was the rate-determining step at the basic pH region, was independent of salt concentration. Conversely, rapid-phase ligand-binding experiments showed that the amplitude of the DHF-binding reaction increased and the tetrahydrofolate (THF)-releasing rate decreased with increasing NaCl concentration. These results suggested that the salt-activation mechanism of HjDHFR P1 is via the population change of the anion-unbound and anion-bound conformers, which are binding-incompetent and -competent conformations for DHF, respectively, while that of salt inactivation is via deceleration of the THF-releasing rate, which is the rate-determining step at the neutral pH region.