WorldWideScience

Sample records for corn maize

  1. Processing maize flour and corn meal food products

    OpenAIRE

    2013-01-01

    Corn is the cereal with the highest production worldwide and is used for human consumption, livestock feed, and fuel. Various food technologies are currently used for processing industrially produced maize flours and corn meals in different parts of the world to obtain precooked refined maize flour, dehydrated nixtamalized flour, fermented maize flours, and other maize products. These products have different intrinsic vitamin and mineral contents, and their processing follows different pathwa...

  2. 19 CFR 10.57 - Certified seed potatoes, and seed corn or maize.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Certified seed potatoes, and seed corn or maize... Provisions Potatoes, Corn, Or Maize § 10.57 Certified seed potatoes, and seed corn or maize. Claim for classification as seed potatoes under subheading 0701.10.00, as seed corn (maize) under subheading...

  3. Processing maize flour and corn meal food products.

    Science.gov (United States)

    Gwirtz, Jeffrey A; Garcia-Casal, Maria Nieves

    2014-04-01

    Corn is the cereal with the highest production worldwide and is used for human consumption, livestock feed, and fuel. Various food technologies are currently used for processing industrially produced maize flours and corn meals in different parts of the world to obtain precooked refined maize flour, dehydrated nixtamalized flour, fermented maize flours, and other maize products. These products have different intrinsic vitamin and mineral contents, and their processing follows different pathways from raw grain to the consumer final product, which entail changes in nutrient composition. Dry maize mechanical processing creates whole or fractionated products, separated by anatomical features such as bran, germ, and endosperm. Wet maize processing separates by chemical compound classification such as starch and protein. Various industrial processes, including whole grain, dry milling fractionation, and nixtamalization, are described. Vitamin and mineral losses during processing are identified and the nutritional impacts outlined. Also discussed are the vitamin and mineral contents of corn.

  4. Processing maize flour and corn meal food products

    Science.gov (United States)

    Gwirtz, Jeffrey A; Garcia-Casal, Maria Nieves

    2014-01-01

    Corn is the cereal with the highest production worldwide and is used for human consumption, livestock feed, and fuel. Various food technologies are currently used for processing industrially produced maize flours and corn meals in different parts of the world to obtain precooked refined maize flour, dehydrated nixtamalized flour, fermented maize flours, and other maize products. These products have different intrinsic vitamin and mineral contents, and their processing follows different pathways from raw grain to the consumer final product, which entail changes in nutrient composition. Dry maize mechanical processing creates whole or fractionated products, separated by anatomical features such as bran, germ, and endosperm. Wet maize processing separates by chemical compound classification such as starch and protein. Various industrial processes, including whole grain, dry milling fractionation, and nixtamalization, are described. Vitamin and mineral losses during processing are identified and the nutritional impacts outlined. Also discussed are the vitamin and mineral contents of corn. PMID:24329576

  5. Yield performance of the European Union Maize Landrace Core Collection under multiple corn borer infestations

    OpenAIRE

    Malvar Pintos, Rosa Ana; Butrón Gómez, Ana María; Álvarez Rodríguez, Ángel; Padilla Alonso, Guillermo; Cartea González, María Elena; Revilla Temiño, Pedro; Ordás Pérez, Amando

    2007-01-01

    In Europe, corn borer attack is the main biotic stressor for the maize (Zea mays L.) crop. European corn borer (Ostrinia nubilalis Hbn.) is the most important maize pest in central and north Europe, while pink stem borer (Sesamia nonagrioides Lef.) is predominant in warmer areas of southern Europe. The objective of this study was the evaluation of the European Maize Union Landrace Core Collection (EUMLCC) for yield under infestation with European corn borer (O. nubilalis) and pink stem borer ...

  6. Western corn rootworm and Bt maize: challenges of pest resistance in the field.

    Science.gov (United States)

    Gassmann, Aaron J; Petzold-Maxwell, Jennifer L; Keweshan, Ryan S; Dunbar, Mike W

    2012-01-01

    Crops genetically engineered to produce insecticidal toxins from the bacterium Bacillus thuringiensis (Bt) manage many key insect pests while reducing the use of conventional insecticides. One of the primary pests targeted by Bt maize in the United States is the western corn rootworm, Diabrotica virgifera virgifera LeConte. Beginning in 2009, populations of western corn rootworm were identified in Iowa, USA that imposed severe root injury to Cry3Bb1 maize. Subsequent laboratory bioassays revealed that these populations were resistant to Cry3Bb1 maize, with survival on Cry3Bb1 maize that was three times higher than populations not associated with such injury. Here we report the results of research that began in 2010 when western corn rootworm were sampled from 14 fields in Iowa, half of which had root injury to Cry3Bb1 maize of greater than 1 node. Of these samples, sufficient eggs were collected to conduct bioassays on seven populations. Laboratory bioassays revealed that these 2010 populations had survival on Cry3Bb1 maize that was 11 times higher and significantly greater than that of control populations, which were brought into the laboratory prior to the commercialization of Bt maize for control of corn rootworm. Additionally, the developmental delays observed for control populations on Cry3Bb1 maize were greatly diminished for 2010 populations. All 2010 populations evaluated in bioassays came from fields with a history of continuous maize production and between 3 and 7 y of Cry3Bb1 maize cultivation. Resistance to Cry34/35Ab1 maize was not detected and there was no correlation between survival on Cry3Bb1 maize and Cry34/35Ab1 maize, suggesting a lack of cross resistance between these Bt toxins. Effectively dealing with the challenge of field-evolved resistance to Bt maize by western corn rootworm will require better adherence to the principles of integrated pest management.

  7. Field-evolved resistance by western corn rootworm to multiple Bacillus thuringiensis toxins in transgenic maize.

    Science.gov (United States)

    Gassmann, Aaron J; Petzold-Maxwell, Jennifer L; Clifton, Eric H; Dunbar, Mike W; Hoffmann, Amanda M; Ingber, David A; Keweshan, Ryan S

    2014-04-01

    The widespread planting of crops genetically engineered to produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) places intense selective pressure on pest populations to evolve resistance. Western corn rootworm is a key pest of maize, and in continuous maize fields it is often managed through planting of Bt maize. During 2009 and 2010, fields were identified in Iowa in which western corn rootworm imposed severe injury to maize producing Bt toxin Cry3Bb1. Subsequent bioassays revealed Cry3Bb1 resistance in these populations. Here, we report that, during 2011, injury to Bt maize in the field expanded to include mCry3A maize in addition to Cry3Bb1 maize and that laboratory analysis of western corn rootworm from these fields found resistance to Cry3Bb1 and mCry3A and cross-resistance between these toxins. Resistance to Bt maize has persisted in Iowa, with both the number of Bt fields identified with severe root injury and the ability western corn rootworm populations to survive on Cry3Bb1 maize increasing between 2009 and 2011. Additionally, Bt maize targeting western corn rootworm does not produce a high dose of Bt toxin, and the magnitude of resistance associated with feeding injury was less than that seen in a high-dose Bt crop. These first cases of resistance by western corn rootworm highlight the vulnerability of Bt maize to further evolution of resistance from this pest and, more broadly, point to the potential of insects to develop resistance rapidly when Bt crops do not achieve a high dose of Bt toxin.

  8. Sources of resistance to pink stem borer and european corn borer in maize

    OpenAIRE

    Malvar Pintos, Rosa Ana; Cartea González, María Elena; Revilla Temiño, Pedro; Ordás Pérez, Amando; Álvarez Rodríguez, Ángel; Mansilla, J.P.

    1993-01-01

    The European corn borer (Ostrinia nubilalis Hbn) is an important insect pest of maize (Zea mays L.) in Europe. However, the larvae of pink stem borer (Sesamia nonagrioides Lef.) produce important damage to the stems, especially in Southern Europe. The first step in an insect-resistance breeding program is to identify sources of resistance. The objective of this work was to study the resistance/tolerance of several populations of maize to attack by corn borers in general, and of Sesamia nonagr...

  9. Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut

    Science.gov (United States)

    The corn smut fungus, Ustilago maydis, is a global pathogen responsible for extensive agricultural losses. Control of corn smut using traditional breeding has met with limited success because natural resistance to U. maydis is organ specific and involves numerous maize genes. Here, we present a tran...

  10. Biofuels derived from corn; Biocombustibles derivados del maiz

    Energy Technology Data Exchange (ETDEWEB)

    Arvizu Fernandez, Jose Luis [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2012-06-22

    The biofuels can be obtained from the biomass, are liquid, solid or gaseous from matter of vegetal or animal origin, by biological, chemical or physical processes and combinations of them. The biofuels appear as an alternating fuels in the transport sector, mainly due to the positive impact of greenhouse gases effect (GHG) and to the increase of oil prices. In theory the use of bioethanol and the biofuels, they not have CO{sub 2} emissions since this it is absorbed in the same amount by the cultures that generate them, as the corn or sugar cane. Our country has a deficit in the food production, mainly in corn, grain that can offer the opportunity not only to continue being the base of the national feeding, but also the raw material to produce bioethanol. The national yields are so diverse that they resemble to those of EUA or Africa, without a doubt this is indicative that is due to devise the way of increase the yields of this appraised culture, reach in average the six tons by hectare, improving the culture practice and access to fertilizers, water, transport, transparent markets and right prices. [Spanish] Los biocombustibles pueden ser obtenidos de la biomasa, son liquidos, solidos o gaseosos provenientes de material vegetal u origen animal, pueden obtenerse mediante procesos biologicos, quimicos o fisicos, y combinaciones de ellos. Se presentan como combustibles alternos en el sector transporte, debido principalmente al impacto positivo de la reduccion de gases de efecto invernadero (GEI) y al aumento de los precios del petroleo. En teoria el uso del bioetanol y los biocombustibles no produce emisiones de CO{sub 2} porque este se absorbe en la misma cantidad a traves de los cultivos que los generan, como el maiz y la cana de azucar. Mexico tiene deficit en la produccion de alimentos, principalmente maiz, grano que puede ofrecer la oportunidad no solo de continuar siendo la base de la alimentacion nacional, sino tambien la materia prima para producir bioetanol

  11. Native Resistance of Maize to Western Corn Rootworm Larval Feeding

    Science.gov (United States)

    The western corn rootworm (WCR) is a major insect pest in continuous corn production. By feeding on corn roots, WCR causes economic losses due to plant lodging and decreased nutrient uptake. Currently, insecticides and transgenic corn are only available options for its control under continuous cor...

  12. Field Evaluation of the Asian Corn Borer Control in Hybrid of Transgenic Maize Event MON 810

    Institute of Scientific and Technical Information of China (English)

    HE Kang-lai; WANG Zhen-ying; WEN Li-ping; BAI Shu-xiong; ZHOU Da-rong; ZHU Qing-hua

    2003-01-01

    In this study,a transgenic Bt maize hybrid(event MON 810 from Monsanto Company)expressing Cry1Ab protein derived from Bacillus thuringiensis(Bt)and its negative isoline hybrid were evaluatrial. Maize plants were artificially infested with neonate larvae of Asian corn borer at the mid-whorl(firstgeneration),pre-tassel(first-and/or second-generation),and silk(second-generation)growth stages.The transgenic Bt maize hybrid sustained significantly less leaf feeding damage(rating 1.0±0.0)than its negative isoline control(rating 7.3±0.1).With the Bt maize,1.3-6.8%of plants were damaged by corn borer tunneling with<0.5 cm tunneling per stalk under different levels of infestation,compared with 100%of plants damaged with 9.3-25.0 cm tunneling per stalk for the negative isoline control. On average,transgenic Bt maize hybrids had only 0.01-0.05 tunnels per stalk and no stems were broken.In contrast,the negative isoline control had 3.11-8.36 tunnels per stalk and 31.2-73.9% of stems broken.Yields were significantly higher in trahsgenic Bt maize than in the control. These results demonstrate that transgenic Bt maize can significantly minimize yield losses caused by the Asian corn borer through resistance to the first-and second-generation larvae.

  13. Field-evolved resistance to Bt maize by western corn rootworm.

    Directory of Open Access Journals (Sweden)

    Aaron J Gassmann

    Full Text Available BACKGROUND: Crops engineered to produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt are planted on millions of hectares annually, reducing the use of conventional insecticides and suppressing pests. However, the evolution of resistance could cut short these benefits. A primary pest targeted by Bt maize in the United States is the western corn rootworm Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae. METHODOLOGY/PRINCIPAL FINDINGS: We report that fields identified by farmers as having severe rootworm feeding injury to Bt maize contained populations of western corn rootworm that displayed significantly higher survival on Cry3Bb1 maize in laboratory bioassays than did western corn rootworm from fields not associated with such feeding injury. In all cases, fields experiencing severe rootworm feeding contained Cry3Bb1 maize. Interviews with farmers indicated that Cry3Bb1 maize had been grown in those fields for at least three consecutive years. There was a significant positive correlation between the number of years Cry3Bb1 maize had been grown in a field and the survival of rootworm populations on Cry3Bb1 maize in bioassays. However, there was no significant correlation among populations for survival on Cry34/35Ab1 maize and Cry3Bb1 maize, suggesting a lack of cross resistance between these Bt toxins. CONCLUSIONS/SIGNIFICANCE: This is the first report of field-evolved resistance to a Bt toxin by the western corn rootworm and by any species of Coleoptera. Insufficient planting of refuges and non-recessive inheritance of resistance may have contributed to resistance. These results suggest that improvements in resistance management and a more integrated approach to the use of Bt crops may be necessary.

  14. Field-evolved resistance to Bt maize by western corn rootworm: predictions from the laboratory and effects in the field.

    Science.gov (United States)

    Gassmann, Aaron J

    2012-07-01

    Crops engineered to produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) provide an effective management tool for many key insect pests. However, pest species have repeatedly demonstrated their ability to adapt to management practices. Results from laboratory selection experiments illustrate the capacity of pest species to evolve Bt resistance. Furthermore, resistance has been documented to Bt sprays in the field and greenhouse, and more recently, by some pests to Bt crops in the field. In 2009, fields were discovered in Iowa (USA) with populations of western corn rootworm, Diabrotica virgifera virgifera LeConte, that had evolved resistance to maize that produces the Bt toxin Cry3Bb1. Fields with resistant insects in 2009 had been planted to Cry3Bb1 maize for at least three consecutive years and as many as 6years. Computer simulation models predicted that the western corn rootworm might evolve resistance to Bt maize in as few as 3years. Laboratory and field data for interactions between western corn rootworm and Bt maize indicate that currently commercialized products are not high-dose events, which increases the risk of resistance evolution because non-recessive resistance traits may enhance survival on Bt maize. Furthermore, genetic analysis of laboratory strains of western corn rootworm has found non-recessive inheritance of resistance. Field studies conducted in two fields identified as harboring Cry3Bb1-resistant western corn rootworm found that survival of western corn rootworm did not differ between Cry3Bb1 maize and non-Bt maize and that root injury to Cry3Bb1 maize was higher than injury to other types of Bt maize or to maize roots protected with a soil insecticide. These first cases of field-evolved resistance to Bt maize by western corn rootworm provide an early warning and point to the need to apply better integrated pest management practices when using Bt maize to manage western corn rootworm.

  15. Early Detection and Mitigation of Resistance to Bt Maize by Western Corn Rootworm (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Andow, David A; Pueppke, Steven G; Schaafsma, Arthur W; Gassmann, Aaron J; Sappington, Thomas W; Meinke, Lance J; Mitchell, Paul D; Hurley, Terrance M; Hellmich, Richard L; Porter, R Pat

    2016-02-01

    Transgenic Bt maize that produces less than a high-dose has been widely adopted and presents considerable insect resistance management (IRM) challenges. Western corn rootworm, Diabrotica virgifera virgifera LeConte, has rapidly evolved resistance to Bt maize in the field, leading to local loss of efficacy for some corn rootworm Bt maize events. Documenting and responding to this resistance has been complicated by a lack of rapid diagnostic bioassays and by regulatory triggers that hinder timely and effective management responses. These failures are of great concern to the scientific and agricultural community. Specific challenges posed by western corn rootworm resistance to Bt maize, and more general concerns around Bt crops that produce less than a high-dose of Bt toxin, have caused uncertainty around current IRM protocols. More than 15 years of experience with IRM has shown that high-dose and refuge-based IRM is not applicable to Bt crops that produce less than a high-dose. Adaptive IRM approaches and pro-active, integrated IRM-pest management strategies are needed and should be in place before release of new technologies that produce less than a high-dose. We suggest changes in IRM strategies to preserve the utility of corn rootworm Bt maize by 1) targeting local resistance management earlier in the sequence of responses to resistance and 2) developing area-wide criteria to address widespread economic losses. We also favor consideration of policies and programs to counteract economic forces that are contributing to rapid resistance evolution.

  16. Technical considerations for maize flour and corn meal fortification in public health: consultation rationale and summary.

    Science.gov (United States)

    Peña-Rosas, Juan Pablo; Garcia-Casal, Maria Nieves; Pachón, Helena; Mclean, Mireille Seneclauze; Arabi, Mandana

    2014-04-01

    Fortification is the purposeful addition of vitamins and minerals to foods during their industrial processing, as a way to improve the nutrition and health of populations who consume these foods. Twelve countries have mandatory maize (Zea mays subsp. Mays) flour or meal fortification. The World Health Organization (WHO) is updating evidence-informed guidelines for the fortification of staple foods in public health, including the fortification of maize flour and corn meal with iron and other micronutrients. Although there is limited experience with fortification of maize, mass fortification of maize flour with at least iron has been practiced for many years in several countries in the Americas and Africa: Brazil, Costa Rica, El Salvador, Kenya, Mexico, Nigeria, Rwanda, South Africa, Tanzania, Uganda, the United States, and Venezuela. The WHO, in collaboration with the Sackler Institute for Nutrition Science and the Flour Fortification Initiative (FFI), convened a consultation on technical considerations for fortification of maize flour and corn meal in public health in New York, New York on April 8-9, 2013 to provide input into the guideline-development process and to discuss technical considerations of the fortification processes for maize flour and corn meal.

  17. Stability of key micronutrients added to fortified maize flours and corn meal.

    Science.gov (United States)

    Dunn, Michael L; Jain, Vijaya; Klein, Barbara P

    2014-04-01

    Maize is a dietary staple in many countries. Although nutritious in many ways and a good source of energy, typical maize lacks several key micronutrients (MNs) that are often added to maize meals or flours to enhance nutritional value. Many factors affect MN stability in maize products, including uncontrolled conditions during distribution, long storage times, and MN premix composition. Consumer preparation also affects the final MN content of food. This review summarizes research relating to MN stability during processing, transport, storage, and meal preparation, focusing on those MNs most often added to maize and maize-based foods. Significant losses in B vitamins (B1, B2, B3, B6, B9, and B12) occur during manufacturing, distribution, and cooking. Added minerals (e.g., iron, zinc, calcium) are generally retained, although phytates in corn may affect bioavailability. Vitamins A and D3 are recent additions to fortification premixes for maize and are not well studied. Although there have been numerous studies of MN fortification in wheat flour, maize has not been as thoroughly examined, so recommendations are not as well supported. Future investigations should include well-designed and executed studies of the most labile MNs added to maize flours and meals, and their fate during all steps of processing, shipping, and preparation. © 2013 New York Academy of Sciences.

  18. Induced defenses in maize following attack by the European corn borer (Ostrinia nubilalis)

    Science.gov (United States)

    Over the course of the past two decades, insect pests such as the European corn borer (Ostrinia nubilalis) annually resulted in losses to US maize production exceeding one billion dollars. Despite the global significance of O. nubilalis and other stem borers, relatively little is known about the nat...

  19. Maize defense response against the european corn borer (Ostrinia nubilaslis): a losing battle?

    Science.gov (United States)

    The goal of this research is to understand how maize stems respond to European corn borer (ECB) damage and how these defense tactics affect the invading ECB. We measured the levels of the plant hormones, jasmonic acid (JA) and ethylene, as well as the transcript levels of their key biosynthetic en...

  20. Diallel Analysis of Southwestern Corn Borer Leaf Feeding Damage in Maize

    Science.gov (United States)

    Southwestern corn borer [Diatraea grandiosella (Dyar)] is an important pest of maize in the southern United States. It feeds extensively within the leaf whorls of plants in the vegetative stages of growth. This reduces both the quantity and quality of harvestable grain. Germplasm lines with resistan...

  1. Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut.

    Science.gov (United States)

    Allen, Aron; Islamovic, Emir; Kaur, Jagdeep; Gold, Scott; Shah, Dilip; Smith, Thomas J

    2011-10-01

    The corn smut fungus, Ustilago maydis, is a global pathogen responsible for extensive agricultural losses. Control of corn smut using traditional breeding has met with limited success because natural resistance to U. maydis is organ specific and involves numerous maize genes. Here, we present a transgenic approach by constitutively expressing the Totivirus antifungal protein KP4, in maize. Transgenic maize plants expressed high levels of KP4 with no apparent negative impact on plant development and displayed robust resistance to U. maydis challenges to both the stem and ear tissues in the greenhouse. More broadly, these results demonstrate that a high level of organ independent fungal resistance can be afforded by transgenic expression of this family of antifungal proteins.

  2. Breeding drought-tolerant maize hybrids for the US corn-belt: discovery to product.

    Science.gov (United States)

    Cooper, Mark; Gho, Carla; Leafgren, Roger; Tang, Tom; Messina, Carlos

    2014-11-01

    Germplasm, genetics, phenotyping, and selection, combined with a clear definition of product targets, are the foundation of successful hybrid maize breeding. Breeding maize hybrids with superior yield for the drought-prone regions of the US corn-belt involves integration of multiple drought-specific technologies together with all of the other technology components that comprise a successful maize hybrid breeding programme. Managed-environment technologies are used to enable scaling of precision phenotyping in appropriate drought environmental conditions to breeding programme level. Genomics and other molecular technologies are used to study trait genetic architecture. Genetic prediction methodology was used to breed for improved yield performance for drought-prone environments. This was enabled by combining precision phenotyping for drought performance with genetic understanding of the traits contributing to successful hybrids in the target drought-prone environments and the availability of molecular markers distributed across the maize genome. Advances in crop growth modelling methodology are being used to evaluate the integrated effects of multiple traits for their combined effects and evaluate drought hybrid product concepts and guide their development and evaluation. Results to date, lessons learned, and future opportunities for further improving the drought tolerance of maize for the US corn-belt are discussed.

  3. Tolerance of Bt corn (MON 810) to maize stem borer, Chilo partellus (Lepidoptera: Pyralidae).

    Science.gov (United States)

    Singh, Ramkumar; Channappa, Ravi K; Deeba, Farah; Nagaraj, Nandi J; Sukavaneaswaran, Mohan K; Manjunath, T M

    2005-11-01

    Transgenic corn (MON 810), expressing the Bacillus thuringiensis (Bt) protein, Cry1Ab, was evaluated under greenhouse conditions for its tolerance to the maize stem borer, Chilo partellus. Bt corn (MON 810) provided effective protection against the stem borer even under a high level of larval infestation in the greenhouse. The observed tolerance is examined and discussed in the light of the susceptibility of C. partellus to the Cry1Ab protein in laboratory bioassays. The implications of the tissue concentrations of Cry1Ab in MON 810, and baseline susceptibility recorded in the current study, for insect-resistance management are discussed.

  4. Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.

    Science.gov (United States)

    Qiu, Shuang; Yadav, Madhav P; Chen, Hao; Liu, Yan; Tatsumi, Eizo; Yin, Lijun

    2015-01-22

    Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing. In this study, the interactions of maize starch and CFG were studied. Maize starch/CFG blend gels were prepared from maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG. The pasting and thermal properties, rheological properties, microstructure, leached amylose and swelling power characteristics were evaluated. Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement. The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased. The thermal characteristics of maize starch/CFG mixtures varied insignificantly as determined in DSC heating process. Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system. Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.

  5. RESOURCE ALLOCATION IN A MAIZE BREEDING PROGRAM FOR NATIVE RESISTANCE TO WESTERN CORN ROOTWORM

    Directory of Open Access Journals (Sweden)

    Ivan Brkić

    2012-06-01

    Full Text Available The objective of this study was to determine the optimum allocation of the number of plants sampled per plot and number of locations and years required for screening maize genotypes for reduced root damage caused by western corn rootworm (WCR larvae, major pest of maize in Croatia, Europe and in the USA. Field trials were conducted on two locations Eastern Croatia, a major maize production area with natural WCR occurrence under continuous maize growing conditions. The trials were set as an incomplete lattice block design in two replications in 2007, 2008 and 2009 including 128 genotypes from various maize gene-pools. Our results suggest that the effect of year and respective interactions including year were the most important factors in maize breeding programs for native resistance to WCR. Thus, screening germplasm for WCR resistance should be made in a multi-year experiment, but not necessarily as a multi-location experiment. Resource optimization should be done by reducing number of roots per plot to minimum 4 sampled plants due to small within-plot environmental variance.

  6. Impact of MON863 transgenic roots is equivalent on western corn rootworm larvae for a wide range of maize phenologies.

    Science.gov (United States)

    Hibbard, Bruce E; El Khishen, Ahmed A; Vaughn, Ty T

    2009-08-01

    The effects of maize, Zea mays L., phenology on establishment, damage, and adult emergence of the western corn rootworm, Diabrotica virgifera virgifera LeConte, on MON863 transgenic maize expressing the Cry3Bb1 protein and its isoline was evaluated in field trials in 2002 and 2003. As expected, plant damage, western corn rootworm larval recovery, and adult emergence were significantly lower on MON863 than isoline maize. The average weight of larvae and adults recovered from MON863 and isoline maize was generally not significantly different. If western corn rootworm larvae were able to establish on transgenic rootworm-resistant plants, larval growth was relatively normal. Plant damage, the number of western corn rootworm larvae recovered, and adult emergence from MON863 did not significantly differ between egg hatch times from widely varying phenologies in either year of the study. Although the extractable level of Cry3Bb1 decreased significantly from vegetative (V)4 to V9 maize in previous studies, in the current study, the amount of Cry3Bb1 did not vary from V3 to R3 in a way that affected neonate survival by western corn rootworm larvae in the field.

  7. Covalent cross-linking of cell-wall polysaccharides through esterified diferulates as a maize resistance mechanism against corn borers.

    Science.gov (United States)

    Barros-Rios, Jaime; Santiago, Rogelio; Jung, Hans-Joachim G; Malvar, Rosa A

    2015-03-04

    There is strong evidence to suggest that cross-linking of cell-wall polymers through ester-linked diferulates has a key role in plant resistance to pests; however, direct experimentation to provide conclusive proof is lacking. This study presents an evaluation of the damage caused by two corn borer species on six maize populations particularly selected for divergent diferulate concentrations in pith stem tissues. Maize populations selected for high total diferulate concentration had 31% higher diferulates than those selected for low diferulates. Stem tunneling by corn borer species was 29% greater in the population with the lowest diferulates than in the population with the highest diferulates (31.7 versus 22.6 cm), whereas total diferulate concentration was negatively correlated with stem tunneling by corn borers. Moreover, orthogonal contrasts between groups of populations evaluated showed that larvae fed in laboratory bioassays on pith stem tissues from maize populations with higher diferulates had 30-40% lower weight than larvae fed on the same tissues from maize populations with lower diferulates. This is the first report that shows a direct relationship between diferulate deposition in maize cell walls and corn borer resistance. Current findings will help to develop adapted maize varieties with an acceptable level of resistance against borers and be useful in special kinds of agriculture, such as organic farming.

  8. Physico-Chemical Characterization of Brew during the Brewing Corn Malt in the Production of Maize Beer in Congo

    OpenAIRE

    P. Diakabana; M. Mvoula-Tsiéri; J. Dhellot; S.C. Kobawila; D. Louembé

    2013-01-01

    The study consists in the production of a traditional beer from maize in the Congo. The traditional method of brewing corn malt has three main stages: malting corn, brewing corn malt and fermentation. During the brewing corn malt, endogenous amylase activity is destroyed during the stiffening of the starch to about 80°C. A pre-cooking of the mash is necessitated to promote amylolyse at 50°C with an exogenous enzyme. The use of a preparation of α-amylase can liquefy the mash and produce a swee...

  9. Cell wall composition as a maize defense mechanism against corn borers.

    Science.gov (United States)

    Barros-Rios, Jaime; Malvar, Rosa A; Jung, Hans-Joachim G; Santiago, Rogelio

    2011-04-01

    European and Mediterranean corn borers are two of the most economically important insect pests of maize (Zea mays L.) in North America and southern Europe, respectively. Cell wall structure and composition were evaluated in pith and rind tissues of resistant and susceptible inbred lines as possible corn borer resistance traits. Composition of cell wall polysaccharides, lignin concentration and composition, and cell wall bound forms of hydroxycinnamic acids were measured. As expected, most of the cell wall components were found at higher concentrations in the rind than in the pith tissues, with the exception of galactose and total diferulate esters. Pith of resistant inbred lines had significantly higher concentrations of total cell wall material than susceptible inbred lines, indicating that the thickness of cell walls could be the initial barrier against corn borer larvae attack. Higher concentrations of cell wall xylose and 8-O-4-coupled diferulate were found in resistant inbreds. Stem tunneling by corn borers was negatively correlated with concentrations of total diferulates, 8-5-diferulate and p-coumarate esters. Higher total cell wall, xylose, and 8-coupled diferulates concentrations appear to be possible mechanisms of corn borer resistance. Copyright © 2011 Elsevier Ltd. All rights reserved.

  10. Effects of application of corn straw on soil microbial community structure during the maize growing season.

    Science.gov (United States)

    Lu, Ping; Lin, Yin-Hua; Yang, Zhong-Qi; Xu, Yan-Peng; Tan, Fei; Jia, Xu-Dong; Wang, Miao; Xu, De-Rong; Wang, Xi-Zhuo

    2015-01-01

    This study investigated the influence of corn straw application on soil microbial communities and the relationship between such communities and soil properties in black soil. The crop used in this study was maize (Zea mays L.). The five treatments consisted of applying a gradient (50, 100, 150, and 200%) of shattered corn straw residue to the soil. Soil samples were taken from May through September during the 2012 maize growing season. The microbial community structure was determined using phospholipid fatty acid (PLFA) analysis. Our results revealed that the application of corn straw influenced the soil properties and increased the soil organic carbon and total nitrogen. Applying corn straw to fields also influenced the variation in soil microbial biomass and community composition, which is consistent with the variations found in soil total nitrogen (TN) and soil respiration (SR). However, the soil carbon-to-nitrogen ratio had no effect on soil microbial communities. The abundance of PLFAs, TN, and SR was higher in C1.5 than those in other treatments, suggesting that the soil properties and soil microbial community composition were affected positively by the application of corn straw to black soil. A Principal Component Analysis indicated that soil microbial communities were different in the straw decomposition processes. Moreover, the soil microbial communities from C1.5 were significantly different from those of CK (p straw treatments that correlated with SR (p straw positively influences soil properties and soil microbial communities and that these properties affect these communities. The individual PLFA signatures were sensitive indicators that reflected the changes in the soil environment condition.

  11. First report of Maize chlorotic mottle virus and maize (corn) lethal necrosis in Kenya

    Science.gov (United States)

    In September 2011, high incidence of a new maize (Zea mays L.) disease was reported at lower elevations (1900 masl) in the Longisa division of Bomet County, Southern Rift Valley of Kenya. Later the disease was noted in Bomet Central division, spreading into the neighboring Chepalungu and Narok South...

  12. EFFICIENCY OF THE CHEMICAL TREATMENT AGAINST THE EUROPEAN CORN BORER IN SEED MAIZE PRODUCTION

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    Emilija Raspudić

    2013-06-01

    Full Text Available The aim of this study was to determine the effectiveness of a chemical treatment against larvae of the European corn borer (Ostrinia nubilalis Hubner. The experiment was set up in 2010 and 2011 in Čepin (eastern Croatia in two treatments: control treatment and insecticide treatment. The trial involved two hybrids of FAO group 400: PR37N01 and PR37F73. Biology of pests was monitored in order to determine population size and larvae development stage as well as the optimal time of insecticide application. After determination of thresholds, maize was treated with chemical formulations of active substance dimethoate. Towards the end of vegetation, length of stem damage, number of larvae in maize stalk and ear as well as grain yield were recorded by dissection of maize stalks. Statistical analysis shows that year, hybrid and chemical treatment significantly influenced the incidence of this pest and justified the use of chemical preparations with mandatory monitoring biology of this pest.

  13. EFFICASY OF NATURAL POPULATION OF Trichogramma WASPS AGAINST EUROPEAN CORN BORER IN FIELD MAIZE

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    Ankica Sarajlić

    2014-12-01

    Full Text Available The aim of this study was to determine the natural infestation of European corn borer (ECB eggs by Trichogramma wasps (Hymenoptera: Trichogrammatidae under field conditions. The experiment was set up in Osijek, Croatia in 2013. The experiment included two levels of irrigations, two nitrogen rates and two maize genotypes. Parameters of ECB feeding activity and maize tolerance (cob mass, tunnel length, number of ECB larvae per plant, as well as number of parasitized ECB eggs by Trihogramma wasps were evaluated. Genotypes were significantly different in terms of tolerance to ECB injury. In treatments with nitrogen fertilization, ECB feeding activity was increased at both nitrogen rates. Agricultural practices did not significantly affect parasitism of ECB eggs by Trichogramma. Correlation between parameters of ECB feeding activity and parasitism by Trichogramma was slight to moderate and not significant. Natural occurrence of Trichogramma wasps were not significantly affected by agricultural practices in maize, and population of these parasitoids was low significantly affect ECB feeding activity.

  14. Plant responses to hidden herbivores: European corn borer (ECB; Ostrinia nubilalis) attack on maize induces both defense and susceptibility

    Science.gov (United States)

    Herbivore-induced plant defenses have been widely described following attack on leaves; however, less attention has been paid to analogous local processes that occur in stems or roots. Early attempts to characterize maize responses to stem boring by European corn borer (ECB; Ostrinia nubilalis) larv...

  15. Datasets for transcriptomic analyses of maize leaves in response to Asian corn borer feeding and/or jasmonic acid

    Directory of Open Access Journals (Sweden)

    Yuliang Zhang

    2016-06-01

    Full Text Available Corn is one of the most widely grown crops throughout the world. However, many corn fields develop pest problems such as corn borers every year that seriously affect its yield and quality. Corn′s response to initial insect damage involves a variety of changes to the levels of defensive enzymes, toxins, and communicative volatiles. Such a dramatic change secondary metabolism necessitates the regulation of gene expression at the transcript level. In this paper, we summarized the datasets of the transcriptome of corn plants in response to corn stalk borers (Ostrinia furnacalis and/or methyl jasmonate (MeJA. Altogether, 39, 636 genes were found to be differentially expressed. The sequencing data are available in the NCBI SRA database under accession number SRS965087. Our dataset will provide more scientific and valuable information for future work such as the study of the functions of important genes or proteins and develop new insect-resistant maize varieties.

  16. Chemical Changes during Maize Tissue Aging and its Relationship with the Mediterranean Corn Borer Resistance.

    Science.gov (United States)

    Lopez-Malvar, Ana; Ordas, Bernardo; Souto, Carlos; Encina, Antonio; Malvar, Rosa Ana; Santiago, Rogelio

    2017-10-02

    Mediterranean Corn Borer (MCB), Sesamia nonagrioides Lef, is an important pest of maize in temperate areas causing significant stalk lodging and yield losses. The main focus of the current study were to determine possible changes in chemical traits (phenols, flavonoids, anthocyanins, sugars, fibers, and lignin) during plant development after the flowering stage, and to assess how those traits may differ in diverse genotypes of maize, MCB resistant and susceptible respectively. Higher values for some particular traits in more mature tissues seemed to increase their effectiveness against the MCB attack. A reduced amount of borer damage in the field was recorded in the resistant inbred line and in older tissues (7.90 cm vs. 31.70 cm as mean for stalk tunnel length). In accordance with these results, the resistant inbred line showed higher hemicellulose cross-linkage (due to ferulic and diferulic acids), soluble sugars content, and higher stalk strength. The use of resistant varieties and early sowings is highly recommended as an integrated approach in order to reduce the yield losses produced by this pest.

  17. Role of the European corn borer (Ostrinia nubilalis) on contamination of maize with 13 Fusarium mycotoxins.

    Science.gov (United States)

    Blandino, Massimo; Scarpino, Valentina; Vanara, Francesca; Sulyok, Michael; Krska, Rudolf; Reyneri, Amedeo

    2015-01-01

    The European corn borer (ECB) plays an important role in promoting Fusarium verticillioides infections and in the consequent fumonisin contamination in maize grain in temperate areas. The objective of this study was to evaluate whether the ECB feeding activity could also affect the occurrence of emerging mycotoxins in maize kernels. During the 2008-10 period, natural infestation of the insect was compared, in field research, with the protection of infestation, which was obtained by using an entomological net. The ears collected in the protected plots were free from ECB attack, while those subject to natural insect attacks showed a damage severity that varied from 10% to 25%. The maize samples were analysed by means of an LC-MS/MS-based multi-mycotoxin method, which led to the detection of various metabolites: fumonisins (FUMs), fusaproliferin (FUS), moniliformin (MON), bikaverin (BIK), beauvericin (BEA), fusaric acid (FA), equisetin (EQU), deoxynivalenol (DON), deoxynivalenol-3-glucoside (DON-3-G), zearalenone (ZEA), culmorin (CULM), aurofusarin (AUR) and butenolide (BUT). The occurrence of mycotoxins produced by Fusarium spp. of Liseola section was affected significantly by the ECB feeding activity. The presence of ECB injuries increased the FUMs from 995 to 4694 µg kg(-1), FUS from 17 to 1089 µg kg(-1), MON from 22 to 673 µg kg(-1), BIK from 58 to 377 µg kg(-1), BEA from 6 to 177 µg kg(-1), and FA from 21 to 379 µg kg(-1). EQU, produced by F. equiseti section Gibbosum, was also increased by the ECB activity, by 1-30 µg kg(-1) on average. Instead, the content of mycotoxins produced by Fusarium spp. of Discolor and Roseum sections was not significantly affected by ECB activity. As for FUMs, the application of a strategy that can reduce ECB damage could also be the most effective solution to minimise the other mycotoxins produced by Fusarium spp. of Liseola section.

  18. crw1--A novel maize mutant highly susceptible to foliar damage by the western corn rootworm beetle.

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    Bala Puchakayala Venkata

    Full Text Available Western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae, is the most destructive insect pest of corn (Zea mays L. in the United States. The adult WCR beetles derive their nourishment from multiple sources including corn pollen and silks as well as the pollen of alternate hosts. Conversely, the corn foliage is largely neglected as a food source by WCR beetles, leading to a perception of a passive interaction between the two. We report here a novel recessive mutation of corn that was identified and named after its foliar susceptibility to corn rootworm beetles (crw1. The crw1 mutant under field conditions was exceptionally susceptible to foliar damage by WCR beetles in an age-specific manner. It exhibits pleiotropic defects on cell wall biochemistry, morphology of leaf epidermal cells and lower structural integrity via differential accumulation of cell wall bound phenolic acids. These findings indicate that crw1 is perturbed in a pathway that was not previously ascribed to WCR susceptibility, as well as implying the presence of an active mechanism(s deterring WCR beetles from devouring corn foliage. The discovery and characterization of this mutant provides a unique opportunity for genetic analysis of interactions between maize and adult WCR beetles and identify new strategies to control the spread and invasion of this destructive pest.

  19. Studies on the Removal of Red Industrial Dye Using Teak Leaf, Maize Corn and Babool Tree Bark Carbons -A Comparison

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    N. Kannan

    2010-01-01

    Full Text Available Activated carbons prepared from teak leaf (TLC, maize corn (MCC and babool tree bark (BTBC were used to study adsorption of red industrial dye under various experimental conditions. Effect of various experimental parameters such as initial concentration, adsorbent dosage, particle size, contact time and initial pH of solution was studied. Batch adsorption studies were carried out at room temperature (30±1°C. Adsorption parameters were modeled by Freundlich and Langmuir isotherm models. Adsorption data were fitted with the Natarajan and Khalaf, Lagergren and Bhattacharya -Venkobachar equations. The high value of 21.28 was obtained from Langmuir plot indicates maize corn carbon (MCC is the best low cost adsorbent. The adsorption process followed first order kinetics, with intra- particle diffusion as one of the rate limiting steps

  20. Host-specific salivary elicitor(s) of European corn borer induce defenses in tomato and maize.

    Science.gov (United States)

    Louis, Joe; Peiffer, Michelle; Ray, Swayamjit; Luthe, Dawn S; Felton, Gary W

    2013-07-01

    Plants turn on induced defenses upon insect herbivory. In the current study, we evaluated the role of European corn borer (ECB) elicitors (molecules secreted by herbivores) that either induce/suppress defenses in Solanum lycopersicum (tomato) and Zea mays (maize), two very important crop plants that are grown for food and/or fuel throughout the world. We used a combination of molecular, biochemical, confocal and scanning electron microscopy, caterpillar spinneret ablation/cauterization, and conventional insect bioassay methods to determine the role of ECB elicitors in modulating defenses in both tomato and maize crop plants. Our results clearly demonstrate that the components present in the ECB saliva induce defense-related proteinase inhibitors in both tomato (PIN2) and maize (MPI). Presence of glucose oxidase in the ECB saliva induced defenses in tomato, but not in maize. However, ECB saliva induced genes present in the jasmonic acid biosynthesis pathway in both tomato and maize. Although ECB saliva can induce defenses in both tomato and maize, our results suggest that host-specific salivary components are responsible for inducing host plant defenses. Proteomic analysis of ECB salivary elicitors and plant receptors/signaling mechanisms involved in recognizing different ECB elicitors remains to be determined. © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

  1. Utilization of Bioslurry on Maize Hydroponic Fodder as a Corn Silage Supplement on Nutrient Digestibility and Milk Production of Dairy Cows

    OpenAIRE

    H. D. Nugroho; I.G. Permana; Despal

    2015-01-01

    The research was conducted to study the effect of addition of 7% DM maize hydroponic fodder (MHF) in corn silage on digestibility and milk production of dairy cows. The experiment used a completely randomized block design with two treatments, and four replications. The treatments were dairy cows fed with grass (Pennisetum purpureum), corn silage, and concentrate (R0), and dairy cows fed with grass (P. purpureum), corn silage, concentrate, and MHF (R1). This research used eight dairy cows with...

  2. Maize bush stunt and corn stunt: Diseases of corn caused by molicutes/ Enfezamentos vermelho e pálido: Doenças em milho causadas por molicutes

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    Nelson Sidnei Massola Júnior

    2001-05-01

    Full Text Available Maize Bushy Stunt and Corn Stunt are diseases of corn caused respectively by a phytoplasma (maize bushy stunt phytoplasma and by Spiroplasma kunkelii. Both agents are restricted to the floem vessels of diseased plants. The leafhopper Dalbulus maidis is the vector of the diseases. The diseases are very harmfull to corn crops and can cause severe losses. Their importance increased very much in the last years mainly due to the continuous crops, which allow the perpetuation of corn, pathogens and vector over the year. Diseased plants show shortening, redening or yellowing and excessive proliferation of ears, among others symptoms. However, symptoms are variable according to the causal agent, environmental conditions and corn genotype. Correct diagnosis of the diseases has been reached with ELISA (“enzyme-linked immunosorbent assay” and PCR (“polymerase chain reaction”. The use of resistant genotypes of corn is the main strategy of control of the diseases.Os enfezamentos vermelho e pálido são doenças do milho causadas, respectivamente, por um fitoplasma (“maize bushy stunt phytoplasma” e pelo Spiroplasma kunkelii, organismos restritos ao floema das plantas infectadas. Ambas são transmitidas pela cigarrinha Dalbulus maidis. São doenças bastante destrutivas, podendo causar sérios prejuízos aos agricultores. A importância dessas doenças aumentou muito nos últimos anos, devido principalmente aos cultivos “safrinha”, que perpetuam o milho, os patógenos e o vetor no campo durante o ano todo. As plantas doentes exibem redução de crescimento, avermelhamento ou amarelecimento, proliferação excessiva de espigas pequenas e improdutivas, além de outros sintomas. No entanto, esses sintomas dependem do agente causal, condições climáticas e genótipo do milho. A diagnose correta tem sido feita por testes de ELISA (“enzyme-linked immunosorbent assay” e por PCR (“polymerase chain reaction”, devido à complexidade da

  3. European corn borer (Ostrinia nubilalis) induced responses enhance susceptibility in maize.

    Science.gov (United States)

    Dafoe, Nicole J; Thomas, James D; Shirk, Paul D; Legaspi, Michelle E; Vaughan, Martha M; Huffaker, Alisa; Teal, Peter E; Schmelz, Eric A

    2013-01-01

    Herbivore-induced plant responses have been widely described following attack on leaves; however, less attention has been paid to analogous local processes that occur in stems. Early studies of maize (Zea mays) responses to stem boring by European corn borer (ECB, Ostrinianubilalis) larvae revealed the presence of inducible acidic diterpenoid phytoalexins, termed kauralexins, and increases in the benzoxazinoid 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one-glucose (HDMBOA-Glc) after 24 h of herbivory. Despite these rapidly activated defenses, larval growth was not altered in short-term feeding assays. Unexpectedly, ECB growth significantly improved in assays using stem tissue preconditioned by 48 h of larval tunneling. Correspondingly, measures of total soluble protein increased over 2.6-fold in these challenged tissues and were accompanied by elevated levels of sucrose and free linoleic acid. While microarray analyses revealed up-regulation of over 1100 transcripts, fewer individual protein increases were demonstrable. Consistent with induced endoreduplication, both wounding and ECB stem attack resulted in similar significant expansion of the nucleus, nucleolus and levels of extractable DNA from challenged tissues. While many of these responses are triggered by wounding alone, biochemical changes further enhanced in response to ECB may be due to larval secreted effectors. Unlike other Lepidoptera examined, ECB excrete exceedingly high levels of the auxin indole-3-acetic acid (IAA) in their frass which is likely to contact and contaminate the surrounding feeding tunnel. Stem exposure to a metabolically stable auxin, such as 2,4-dichlorophenoxyacetic acid (2,4-D), promoted significant protein accumulation above wounding alone. As a future testable hypothesis, we propose that ECB-associated IAA may function as a candidate herbivore effector promoting the increased nutritional content of maize stems.

  4. European corn borer (Ostrinia nubilalis induced responses enhance susceptibility in maize.

    Directory of Open Access Journals (Sweden)

    Nicole J Dafoe

    Full Text Available Herbivore-induced plant responses have been widely described following attack on leaves; however, less attention has been paid to analogous local processes that occur in stems. Early studies of maize (Zea mays responses to stem boring by European corn borer (ECB, Ostrinianubilalis larvae revealed the presence of inducible acidic diterpenoid phytoalexins, termed kauralexins, and increases in the benzoxazinoid 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one-glucose (HDMBOA-Glc after 24 h of herbivory. Despite these rapidly activated defenses, larval growth was not altered in short-term feeding assays. Unexpectedly, ECB growth significantly improved in assays using stem tissue preconditioned by 48 h of larval tunneling. Correspondingly, measures of total soluble protein increased over 2.6-fold in these challenged tissues and were accompanied by elevated levels of sucrose and free linoleic acid. While microarray analyses revealed up-regulation of over 1100 transcripts, fewer individual protein increases were demonstrable. Consistent with induced endoreduplication, both wounding and ECB stem attack resulted in similar significant expansion of the nucleus, nucleolus and levels of extractable DNA from challenged tissues. While many of these responses are triggered by wounding alone, biochemical changes further enhanced in response to ECB may be due to larval secreted effectors. Unlike other Lepidoptera examined, ECB excrete exceedingly high levels of the auxin indole-3-acetic acid (IAA in their frass which is likely to contact and contaminate the surrounding feeding tunnel. Stem exposure to a metabolically stable auxin, such as 2,4-dichlorophenoxyacetic acid (2,4-D, promoted significant protein accumulation above wounding alone. As a future testable hypothesis, we propose that ECB-associated IAA may function as a candidate herbivore effector promoting the increased nutritional content of maize stems.

  5. Effect of MIR604 transgenic maize at different stages of development on western corn rootworm (Coleoptera: Chrysomelidae) in a central Missouri field environment.

    Science.gov (United States)

    Frank, Daniel L; Bukowsky, Rebecca; French, B Wade; Hibbard, Bruce E

    2011-12-01

    The establishment and survival of western corn rootworm, Diabrotica virgifera virgifera LeConte, was evaluated on transgenic Bacillus thuringiensis Berliner maize, Zea mays L., expressing the mCry3A protein (MIR604) and non-Bt maize with the same genetic background (isoline maize) at different stages of development in 2007 and 2008. Overall, western corn rootworm larval recovery, root damage, and adult emergence were significantly higher on isoline maize compared with MIR604. The number of larvae and adults collected from MIR604 did not significantly differ among egg hatch dates from each maize developmental stage evaluated in either year. In 2007, damage to isoline maize roots was lower than expected and never exceeded 0.24 nodes of damage. In 2008, over 0.60 nodes of damage occurred on isoline maize roots. The mean weight and head capsule width of larvae and adults recovered from MIR604 and isoline maize were generally not significantly different. Results are discussed in relation to insect resistance management of western corn rootworm.

  6. Volatiles induced by the larvae of the Asian corn borer (Ostrinia furnacalis) in maize plants affect behavior of conspecific larvae and female adults

    Institute of Scientific and Technical Information of China (English)

    Cui-Hong Huang; Feng-Ming Yan; John A.Byers; Rong-Jiang Wang; Chong-Ren Xu

    2009-01-01

    Effects of maize (Zea mays L.) volatiles induced by larvae of the Asian corn borer, Ostrinia furnacalis (Guenee), on the orientation behaviors of Asian corn borer larvae and oviposition of the females were investigated. Nineteen volatile chemicals, with terpenes being the major components, were identified from maize plants attacked by third instar Asian corn borer larvae. Coupled gas chromatographic-electroantennographic detection (GC-EAD) analyses revealed some electroantennographic differences between female and male Asian corn borer antennae in response to larvae-induced maize volatiles; female responded to (E)-2-hexenal, nonanal, (Z)-3-hexen-1-ol and three unknown compounds while the male only responded to (E)-2-hexenal, nonanal and one unknown compound. In laboratory orientation bioassays, Asian corn borer neonate larvae were attracted to extracts collected from Asian corn borer-damaged plants as well as to synthetic famesene, but were repelled by (Z)-3-hexen- 1-ol. In laboratory oviposition bioassays, gravid females laid fewer eggs on plants damaged by larvae than on mechanically damaged plants or undamaged plants. Adult Asian corn borer females deposited fewer eggs on wax paper treated with (E)-2-hexenal or (Z)-3-hexen-l-ol than on wax paper treated with hexane (control). The results suggest that Asian corn borer can affect the behaviors of conspecific larvae and adults by changing host plant volatiles.

  7. Multitrophic Interaction in the Rhizosphere of Maize: Root Feeding of Western Corn Rootworm Larvae Alters the Microbial Community Composition

    Science.gov (United States)

    Dematheis, Flavia; Zimmerling, Ute; Flocco, Cecilia; Kurtz, Benedikt; Vidal, Stefan; Kropf, Siegfried; Smalla, Kornelia

    2012-01-01

    Background Larvae of the Western Corn Rootworm (WCR) feeding on maize roots cause heavy economical losses in the US and in Europe. New or adapted pest management strategies urgently require a better understanding of the multitrophic interaction in the rhizosphere. This study aimed to investigate the effect of WCR root feeding on the microbial communities colonizing the maize rhizosphere. Methodology/Principal Findings In a greenhouse experiment, maize lines KWS13, KWS14, KWS15 and MON88017 were grown in three different soil types in presence and in absence of WCR larvae. Bacterial and fungal community structures were analyzed by denaturing gradient gel electrophoresis (DGGE) of the16S rRNA gene and ITS fragments, PCR amplified from the total rhizosphere community DNA. DGGE bands with increased intensity were excised from the gel, cloned and sequenced in order to identify specific bacteria responding to WCR larval feeding. DGGE fingerprints showed that the soil type and the maize line influenced the fungal and bacterial communities inhabiting the maize rhizosphere. WCR larval feeding affected the rhiyosphere microbial populations in a soil type and maize line dependent manner. DGGE band sequencing revealed an increased abundance of Acinetobacter calcoaceticus in the rhizosphere of several maize lines in all soil types upon WCR larval feeding. Conclusion/Significance The effects of both rhizosphere and WCR larval feeding seemed to be stronger on bacterial communities than on fungi. Bacterial and fungal community shifts in response to larval feeding were most likely due to changes of root exudation patterns. The increased abundance of A. calcoaceticus suggested that phenolic compounds were released upon WCR wounding. PMID:22629377

  8. Corn performance with late sowing in Planalto Catarinense, Brazil, simulated with CERES-maize model

    Directory of Open Access Journals (Sweden)

    Célio Orli Cardoso

    2008-08-01

    Full Text Available This work had the objective to investigate the delay effects in the sowing date on corn establishment, cycle duration and yield through the computer simulations using CERES-Maize model, in order to help agrarian producers from the Mountainous Area of Santa Catarina, Brazil to take better decisions on it. The computer simulations showed that: 1the risks on corn establishment increased due to the delay of sowing during the studied years; 2 in some years, its cycles were drastically affected for the lower temperatures; 3 the cycle durations were longer when confronted with the low temperatures; 4 corn income decreased when the cycles were confronted with the low temperatures, water deficiency and low solar radiation; 5 potential incomes averaged 4944 kg ha-1, and under the natural rain conditions averaged 2490 kg ha-1, during all the months analyzed from October to March; 6 besides January, the exploration of this cultivar was not viable because of the high risk of the crop frustration associated to the same ones, due to the thermal conditions (low temperatures and frost.O trabalho teve como objetivo investigar os efeitos decorrentes do atraso da semeadura no estabelecimento, duração do ciclo e rendimento do milho, mediante simulações em computador com o uso do modelo CERES-Maize, a fim de orientar os produtores na tomada de decisão na região do Planalto Serrano Catarinense. Em função da época em que é cultivado, o milho com semeadura tardia normalmente está exposto a riscos maiores de perdas por geadas e defici��ncia hídrica em relação ao cultivo na época recomendada, atingindo um menor potencial de produtividade e tendência de alongamento do ciclo. As simulações permitiram concluir que: os riscos de estabelecimento do milho foram bastante variáveis ao longo dos decêndios analisados; em alguns anos os ciclos foram afetados drasticamente por temperaturas bastante baixas; as durações dos ciclos tenderam a alongar-se quando

  9. Physico-Chemical Characterization of Brew during the Brewing Corn Malt in the Production of Maize Beer in Congo

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    P. Diakabana

    2013-06-01

    Full Text Available The study consists in the production of a traditional beer from maize in the Congo. The traditional method of brewing corn malt has three main stages: malting corn, brewing corn malt and fermentation. During the brewing corn malt, endogenous amylase activity is destroyed during the stiffening of the starch to about 80°C. A pre-cooking of the mash is necessitated to promote amylolyse at 50°C with an exogenous enzyme. The use of a preparation of α-amylase can liquefy the mash and produce a sweet wort (average density = 12.5° Balling rich in dextrin corresponding to an apparent extract of 4° Balling in beer. The rising profile of the pH of the corn malt mash, from mashing to extract the wort does not affect the pH of the beer produced. This beer, slightly alcoholic (3.6% ethanol, is characterized by a nomal acid pH (pH = 4.15 on average and a brown color (25 EBC units. Its slight bitterness (21 EBU and the fine aroma of a beer closer barley produced industrially in the Congo.

  10. Assessment of Inheritance and Fitness Costs Associated with Field-Evolved Resistance to Cry3Bb1 Maize by Western Corn Rootworm

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    Aubrey R. Paolino

    2017-05-01

    Full Text Available The western corn rootworm, Diabrotica virgifera virgifera LeConte, is among the most serious insect pests of maize in North America. One strategy used to manage this pest is transgenic maize that produces one or more crystalline (Cry toxins derived from the bacterium Bacillus thuringiensis (Bt. To delay Bt resistance by insect pests, refuges of non-Bt maize are grown in conjunction with Bt maize. Two factors influencing the success of the refuge strategy to delay resistance are the inheritance of resistance and fitness costs, with greater delays in resistance expected when inheritance of resistance is recessive and fitness costs are present. We measured inheritance and fitness costs of resistance for two strains of western corn rootworm with field-evolved resistance to Cry3Bb1 maize. Plant-based and diet-based bioassays revealed that the inheritance of resistance was non-recessive. In a greenhouse experiment, in which larvae were reared on whole maize plants in field soil, no fitness costs of resistance were detected. In a laboratory experiment, in which larvae experienced intraspecific and interspecific competition for food, a fitness cost of delayed larval development was identified, however, no other fitness costs were found. These findings of non-recessive inheritance of resistance and minimal fitness costs, highlight the potential for the rapid evolution of resistance to Cry3Bb1 maize by western corn rootworm, and may help to improve resistance management strategies for this pest.

  11. Multitrophic interactions among Western Corn Rootworm, Glomus intraradices and microbial communities in the rhizosphere and endorhiza of maize plants

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    Flavia eDematheis

    2013-12-01

    Full Text Available The complex interactions among the maize pest Western Corn Rootworm (WCR, Glomus intraradices (GI - recently renamed Rhizophagus intraradices and the microbial communities in both rhizosphere and endorhiza of maize have been investigated in view of new pest control strategies. In a greenhouse experiment, different maize treatments were established: C (control plants, W (plants inoculated with WCR, G (plants inoculated with GI, GW (plants inoculated with GI and WCR. After 20 days of WCR root feeding, larval fitness was measured. Dominant arbuscular mycorrhizal fungi (AMFs in soil and maize endorhiza were analysed by cloning of 18S rRNA gene fragments of AMFs, restriction fragment length polymorphism and sequencing. Bacterial and fungal communities in the rhizosphere and endorhiza were investigated by denaturing gradient gel electrophoresis of ITS and 16S rRNA gene fragments, PCR amplified from total community DNA, respectively. GI reduced significantly WCR larval development and affected the naturally occurring endophytic AMFs and bacteria. WCR root feeding influenced the endophytic bacteria as well.GI can be used in integrated pest management programs, rendering WCR larvae more susceptible to predation by natural enemies. The mechanisms behind the interaction between GI and WCR remain unknown. However, our data suggested that GI might act indirectly via plant-mediated mechanisms influencing the endophytic microbial communities.

  12. Multitrophic interactions among Western Corn Rootworm, Glomus intraradices and microbial communities in the rhizosphere and endorhiza of maize.

    Science.gov (United States)

    Dematheis, Flavia; Kurtz, Benedikt; Vidal, Stefan; Smalla, Kornelia

    2013-01-01

    The complex interactions among the maize pest Western Corn Rootworm (WCR), Glomus intraradices (GI-recently renamed Rhizophagus intraradices) and the microbial communities in both rhizosphere and endorhiza of maize have been investigated in view of new pest control strategies. In a greenhouse experiment, different maize treatments were established: C (control plants), W (plants inoculated with WCR), G (plants inoculated with GI), GW (plants inoculated with GI and WCR). After 20 days of WCR root feeding, larval fitness was measured. Dominant arbuscular mycorrhizal fungi (AMF) in soil and maize endorhiza were analyzed by cloning of 18S rRNA gene fragments of AMF, restriction fragment length polymorphism and sequencing. Bacterial and fungal communities in the rhizosphere and endorhiza were investigated by denaturing gradient gel electrophoresis of 16S rRNA gene and ITS fragments, PCR amplified from total community DNA, respectively. GI reduced significantly WCR larval development and affected the naturally occurring endorhiza AMF and bacteria. WCR root feeding influenced the endorhiza bacteria as well. GI can be used in integrated pest management programs, rendering WCR larvae more susceptible to predation by natural enemies. The mechanisms behind the interaction between GI and WCR remain unknown. However, our data suggested that GI might act indirectly via plant-mediated mechanisms influencing the endorhiza microbial communities.

  13. Pretreatment of poultry manure for efficient biogas production as monosubstrate or co-fermentation with maize silage and corn stover.

    Science.gov (United States)

    Böjti, Tamás; Kovács, Kornél L; Kakuk, Balázs; Wirth, Roland; Rákhely, Gábor; Bagi, Zoltán

    2017-08-01

    Water extraction of raw chicken manure elevated the carbon-to-nitrogen ratio 2.7-fold, i.e. from 7.48 to 19.81. The treated chicken manure (T-CM) thus became suitable for biogas fermentation as monosubstrate. Improved methane production was achieved in co-fermentations with either maize silage (24% more methane) or corn stover (19% more methane) relative to T-CM monosubstrate. The standardized biogas potential assay indicated that the methane yields varied with the organic loading rate between 160 and 250 mL CH4/g organic total solid (oTS). Co-fermentation with maize silage was sustainable in continuous anaerobic digestion for at least 4 months. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Diferulate content of maize sheaths is associated with resistance to the Mediterranean corn borer Sesamia nonagrioides (Lepidoptera: Noctuidae).

    Science.gov (United States)

    Santiago, Rogelio; Butrón, Ana; Reid, Lana M; Arnason, John T; Sandoya, German; Souto, Xose C; Malvar, Rosa A

    2006-11-29

    The leaf sheaths of selected inbred lines of maize (Zea mays L.) with variable levels of stem resistance to the Mediterranean corn borer Sesamia nonagrioides (Lefèvbre) were evaluated for antibiotic effect on insect development. Phytochemical analyses of leaf sheaths were conducted for cell wall phenylpropanoid content to gain a better understanding of maize-resistance mechanisms. Laboratory bioassays established that sheath tissues from different genotypes significantly affected the growth of neonate larvae. Three hydroxycinnamates, p-coumaric, trans-ferulic, and cis-ferulic acids, and three isomers of diferulic acid, 8-5', 8-O-4', and 8-5' b (benzofuran form), were identified. Significant negative correlations were found between larvae weight and diferulic acid content for six genotypes. These results are in agreement with previous studies concerning the role of cell wall structural components in stem borer resistance.

  15. Comparison of fumonisin contamination using HPLC and ELISA methods in Bt and near-isogenic maize hybrids infested with European corn borer or Western bean cutworm

    Science.gov (United States)

    Field trials were conducted (2007 to 2010) to compare grain fumonisin levels among non-Bt maize hybrids and Bt hybrids with transgenic protection against European corn borer and Western bean cutworm (WBC). High-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA) w...

  16. Resistance Identification of Maize Germplasms to Corn Borer%玉米种质资源抗螟性鉴定

    Institute of Scientific and Technical Information of China (English)

    李青青; 郭成; 郭满库; 魏宏玉; 郭建国

    2014-01-01

    为明确玉米不同品种(系)之间的抗螟性差异,采用田间自然感螟法,于2010~2011年在甘肃省天水市甘谷县对478份玉米种质资源进行田间抗性鉴定。结果表明:在216份农家种资源、216份自交系和46份杂交种中共筛选出高抗材料86份、抗螟材料85份、中抗材料167份,分别占供鉴材料的18.0%、17.8%和34.9%;其余140份为感螟和高感材料,占29.3%。%So as to confirm the borer-resistance differences among different corn varieties ( inbred lines ) , the study adopted the method of being naturally infected by Ostrinia furnacalis under field conditions , and identified the resistance of 478 maize germ-plasms to corn borer in Gan ’ gu county, Tianshui city, Gansu province from 2010 to 2012.The results showed that: among 478 maize germplasms (216 peasant variety resources , 216 inbred lines and 46 hybrids), 86 high-resistant materials (18.0% of all materials), 85 borer-resistant materials (17.8%), 167 middle-resistant materials (34.9%) were screened out;the other 140 materials were susceptible or highly susceptible to corn borer , accounting for 29.3%of all tested materials.

  17. [Application of dielectric cooking in the elaboration of instant maize flour for the preparation of corn and tortilla chips].

    Science.gov (United States)

    Gaytán Martínez, M; Martínez-Bustos, F; Morales Sánchez, E

    2000-12-01

    This present work reports on the evaluation of a method for producing of instant flour for corn and tortilla chips based on dielectric cooking (DC). The samples prepared with lime showed a smaller variation in color than those without lime. A greater water absorption capacity was found in the samples processed without lime. The values for cohesion and adhesion of the DC masa prepared with lime are within the range of values previously reported for samples of masa prepared with nixtamal and commercial instant maize flours. The color analyses of chips from DC masa showed slight changes in relation to the chips prepared by the traditional process of nixtamalization. The DC tortilla chips (plus lime) with 15 minutes of DC showed similar values of delta E as compared to the control (commercial tortilla chips). The moisture of the DC chips varied in the range from 1.5 to 2.8. The DC tortilla chips with lime absorbed a lower amount of oil than the control sample and the corn chips. In fact, the DC chips absorbed lower amount of oil as compared to the control. The DC tortilla chips with lime and processed for 10 and 15 minutes were crunchy and smooth. Dielectric cooking displays a high potential for corn and tortilla chips with good functional characteristics, giving substantial savings of water, processing time and with higher yields due to the use of the whole grain.

  18. A challenge for the seed mixture refuge strategy in Bt maize: impact of cross-pollination on an ear-feeding pest, corn earworm.

    Science.gov (United States)

    Yang, Fei; Kerns, David L; Head, Graham P; Leonard, B Rogers; Levy, Ronnie; Niu, Ying; Huang, Fangneng

    2014-01-01

    To counter the threat of insect resistance, Bacillus thuringiensis (Bt) maize growers in the U.S. are required to plant structured non-Bt maize refuges. Concerns with refuge compliance led to the introduction of seed mixtures, also called RIB (refuge-in-the-bag), as an alternative approach for implementing refuge for Bt maize products in the U.S. Maize Belt. A major concern in RIB is cross-pollination of maize hybrids that can cause Bt proteins to be present in refuge maize kernels and negatively affect refuge insects. Here we show that a mixed planting of 5% nonBt and 95% Bt maize containing the SmartStax traits expressing Cry1A.105, Cry2Ab2 and Cry1F did not provide an effective refuge for an important above-ground ear-feeding pest, the corn earworm, Helicoverpa zea (Boddie). Cross-pollination in RIB caused a majority (>90%) of refuge kernels to express ≥ one Bt protein. The contamination of Bt proteins in the refuge ears reduced neonate-to-adult survivorship of H. zea to only 4.6%, a reduction of 88.1% relative to larvae feeding on ears of pure non-Bt maize plantings. In addition, the limited survivors on refuge ears had lower pupal mass and took longer to develop to adults.

  19. A challenge for the seed mixture refuge strategy in Bt maize: impact of cross-pollination on an ear-feeding pest, corn earworm.

    Directory of Open Access Journals (Sweden)

    Fei Yang

    Full Text Available To counter the threat of insect resistance, Bacillus thuringiensis (Bt maize growers in the U.S. are required to plant structured non-Bt maize refuges. Concerns with refuge compliance led to the introduction of seed mixtures, also called RIB (refuge-in-the-bag, as an alternative approach for implementing refuge for Bt maize products in the U.S. Maize Belt. A major concern in RIB is cross-pollination of maize hybrids that can cause Bt proteins to be present in refuge maize kernels and negatively affect refuge insects. Here we show that a mixed planting of 5% nonBt and 95% Bt maize containing the SmartStax traits expressing Cry1A.105, Cry2Ab2 and Cry1F did not provide an effective refuge for an important above-ground ear-feeding pest, the corn earworm, Helicoverpa zea (Boddie. Cross-pollination in RIB caused a majority (>90% of refuge kernels to express ≥ one Bt protein. The contamination of Bt proteins in the refuge ears reduced neonate-to-adult survivorship of H. zea to only 4.6%, a reduction of 88.1% relative to larvae feeding on ears of pure non-Bt maize plantings. In addition, the limited survivors on refuge ears had lower pupal mass and took longer to develop to adults.

  20. Efficiency of insecticide and bioinsecticide treatments against infestation of European corn borer and their effect on maize yield

    Directory of Open Access Journals (Sweden)

    Gošić-Dondo Snežana

    2016-01-01

    Full Text Available During 2008 and 2009, efficacy of insecticidal products, based on thiamethoxam and imidacloprid, were tested. Furthermore, effects of entomophatogenic fungus Beauveria bassiana on the infestation intensity of European corn borer (Ostrinia nubilalis, were established over effects of both, 10 different treatments of maize seed (8 treatments and 2 controls and the application of the fungal inoculum. Trials were carried out on maize crop under field conditions in accordance with the EPPO method. Results obtained in the studies with the product Cruiser 350 FS (350g l-1 thiamethoxam applied in the amount of 0.9 l per 100 kg of seed and the products Imidor 600 FS and Gaucho 600FS (600g l-1 imidacloprid applied in the amount of 0.7 l per 100 kg of seed, point out to satisfactory efficacy in controlling ECB and satisfactory effects on maize yields. The intensity of ECB infestation, expressed in percentages, ranged from 50.1% to 74.1%. The interrelation between average percentages of infested plants and obtained yields in both investigation years (2008-2009 in Zemun Polje was established. Results over treatments indicate that the highest percentages (62.2% of infested plants were observed in the control variant (K1- Captan. Moreover, as expected, the lowest yield was also recorded in this variant. The treatment with the fungicide Maxim combined with the insecticide Criuser proved to be the most efficient. At the same time, the percentage of infested plants was the lowest in this treatment (53.32% and the yield was the highest (8.610 t ha-1. The infestation level of maize plants in this treatment was significantly reduced over years of investigation in comparison with remaining treatments. Meteorological factors during the years of investigation, especially unfavourable agroecological conditions in 2009, affected the average yield of maize per hectare. The yield (8.314 t ha-1 recorded in the treatment with the inoculum of B. basssiana was almost equal to

  1. Identification and Fine Mapping of rhm1 Locus for Resistance to Southern Corn Leaf Blight in Maize

    Institute of Scientific and Technical Information of China (English)

    Yuanzeng Zhao; Xiaomin Lu; Chaoxian Liu; Haiying Guan; Mei Zhang; Zhongfeng Li; Hongwei Cai; Jinsheng Lai

    2012-01-01

    rhm1 is a major recessive disease resistance locus for Southern corn leaf blight (SCLB).To further narrow down its genetic position,F2 population and BC1F1 population derived from the cross between resistant (H95rhm) and susceptible parents (H95) of maize (Zea mays) were constructed.Using newly developed markers,rhm1 was initially delimited within an interval of 2.5 Mb,and then finally mapped to a 8.56 kb interval between InDel marker IDP961-503 and simple sequence repeat (SSR) marker A194149-1.Three polymorphic markers IDP961-504,IDP B2-3 and A194149-2 were shown to be co-segregated with the rhm1 locus.Sequence analysis of the 8.56 kb DNA fragment revealed that it contained only one putative gene with a predicted amino acid sequence identical to lysine histidine transporter 1 (LHT1).Comparative sequence analysis indicated that the LHT1 in H95rhm harbors a 354 bp insertion in its third exon as compared with that of susceptible alleles in B73,H95 and Mo17.The 354 bp insertion resulted in a truncation of the predicted protein of candidate resistance allele (LHT1-H95rhm).Our results strongly suggest LHT1 as the candidate gene for rhm1 against SCLB.The tightly linked molecular markers developed in this study can be directly used for molecular breeding of resistance to Southern corn leaf blight in maize.

  2. Effect of maize lines on larval fitness costs of Cry1F resistance in the European corn borer (Lepidoptera: Crambidae).

    Science.gov (United States)

    Petzold-Maxwell, Jennifer L; Siegfried, Blair D; Hellmich, Richard L; Abel, Craig A; Coates, Brad S; Spencer, Terrence A; Gassmann, Aaron J

    2014-04-01

    Crops producing insecticidal toxins from the bacterium Bacillus thuringiensis (Bt) are widely planted and enable management of key insect pests while reducing the use of conventional insecticides. However, the evolution of Bt resistance could diminish these benefits. Fitness costs of Bt resistance occur in the absence of Bt toxin when individuals with resistance alleles show a reduction in fitness relative to susceptible individuals, and they can delay the evolution of resistance. Ecological factors including host-plant variety can affect the magnitude of fitness costs, and consequently, the degree to which fitness costs delay resistance. In this study, we measured fitness costs of resistance to Bt toxin Cry1F in the European corn borer Ostrinia nubilalis Hübner (Lepidoptera: Crambidae) using Cry1F-resistant and Cry1F-susceptible strains sharing a similar genetic background. Fitness costs were tested on three lines of maize, Zea mays L., by measuring larval survival and development in two greenhouse experiments with plants in either the vegetative or reproductive stage. Both experiments showed that maize line significantly affected larval survival and developmental rate. However, larval survival, mass, and developmental rate did not differ between the Cry1F-resistant and susceptible strains, indicating a lack of fitness costs of resistance to Cry1F for the larval fitness components measured in this experiment. Future experiments should test for fitness costs of Cry1F resistance affecting survival to adulthood and adult life-history parameters.

  3. An investigation on different harvesting methods on young pods of KKU # 922 maize (Zea mays L.) cultivar for baby corn production.

    Science.gov (United States)

    Kasikranan, S

    2011-04-01

    This study aimed to search for the best indicator to be used for the harvest of maize pods for baby corn production. A Randomized Complete Block Design (RCBD) with four replications was used. The treatments are: T1 (Control) Taking sample when silks of female flower had extended from tip of pod up to 3 cm long T2, silks had extended 1 cm long T3, silks had extended 2 cm long T4, blooming of female flower for 2 days T5, blooming of female flowers for 4 days T6, blooming of female flower for 6 days T7, one third blooming of male flower T8, two third blooming of male flower and T9, full bloom of male flower. Five baby corn Characteristics were used i.e., (1) fresh weight of whole ears, (2) fresh weight of ears without husk, (3) commercial standard ears, (4) off standard ears and (5) disordered kernel-rows of ears. A range of scores from 1 to 9 was applied to judge quality and yield in each item of the five baby corn characteristics. A score of 1 = the best whilst further increases in scores indicated the decline in quality of baby corn. The results showed that an indicator for use in harvesting pods of maize for baby corn production was found with T6, i.e. the best time for the harvest of pods is when the female flowers had bloomed for 6 days after the appearance of silks.

  4. Comparison of grain from corn rootworm resistant transgenic DAS-59122-7 maize with non-transgenic maize grain in a 90-day feeding study in Sprague-Dawley rats.

    Science.gov (United States)

    He, X Y; Huang, K L; Li, X; Qin, W; Delaney, B; Luo, Y B

    2008-06-01

    DAS-59122-7 (59122) is a transgenic maize (Zea mays L.) that contains genes encoding Cry34Ab1 and Cry35Ab1 proteins from Bacillus thuringiensis Berliner strain 149B1 and phosphinothricin acetyltransferase (PAT) protein from Streptomyces viridochromogenes. Expression of these proteins in planta confers resistance to corn rootworms and other Coleopteran parasites and tolerance to herbicides containing glufosinate ammonium, respectively. In the current study, processed flours from 59122 maize grain or its near isogenic control line (091) were used at two concentrations (50% and 70% wt/wt) to produce diets that were fed to rats for 90 days in accordance with Chinese toxicology guidelines (GB15193.13-2003). A commercial AIN93G diet was used as an additional negative control. No significant differences in body weight and feed utilization were observed between rats consuming diets formulated with 59122 and 091 Control corn. Statistical differences (p<0.05) were observed in certain hematology and serum chemistry response variables between rats consuming diets formulated with 59122 or 091 Control flour compared to AIN93G diet. However, the mean value of these response variables in the 59122 groups were not statistically different from those observed in diets formulated with corresponding high and low concentrations of the flour from the 091 Control maize grain. Therefore, the statistical differences were considered to be related to consumption of diets containing high concentrations of maize flour (compared to AIN93G diets) regardless of source rather than to consumption of flour from 59122 maize grain. The results from this study demonstrated that 59122 maize grain is as safe as non-transgenic maize grain.

  5. Resistance evolution to the first generation of genetically modified Diabrotica-active Bt-maize events by western corn rootworm: management and monitoring considerations.

    Science.gov (United States)

    Devos, Yann; Meihls, Lisa N; Kiss, József; Hibbard, Bruce E

    2013-04-01

    Western corn rootworm (Diabrotica virgifera virgifera; WCR) is a major coleopteran maize pest in North America and the EU, and has traditionally been managed through crop rotation and broad-spectrum soil insecticides. Genetically modified Bt-maize offers an additional management tool for WCR and has been valuable in reducing insecticide use and increasing farm income. A concern is that the widespread, repeated, and exclusive deployment of the same Bt-maize transformation event will result in the rapid evolution of resistance in WCR. This publication explores the potential of WCR to evolve resistance to plant-produced Bt-toxins from the first generation of Diabrotica-active Bt-maize events (MON 863 and MON 88017, DAS-59122-7 and MIR604), and whether currently implemented risk management strategies to delay and monitor resistance evolution are appropriate. In twelve of the twelve artificial selection experiments reported, resistant WCR populations were yielded rapidly. Field-selected resistance of WCR to Cry3Bb1 is documented in some US maize growing areas, where an increasing number of cases of unexpected damage of WCR larvae to Bt-maize MON 88017 has been reported. Currently implemented insect resistance management measures for Bt-crops usually rely on the high dose/refuge (HDR) strategy. Evidence (including laboratory, greenhouse and field data) indicates that several conditions contributing to the success of the HDR strategy may not be met for the first generation of Bt-maize events and WCR: (1) the Bt-toxins are expressed heterogeneously at a low-to-moderate dose in roots; (2) resistance alleles may be present at a higher frequency than initially assumed; (3) WCR may mate in a non-random manner; (4) resistance traits could have non-recessive inheritance; and (5) fitness costs may not necessarily be associated with resistance evolution. However, caution must be exercised when extrapolating laboratory and greenhouse results to field conditions. Model predictions

  6. Management of fumonisin contamination in maize kernels through the timing of insecticide application against the European corn borer Ostrinia nubilalis Hübner.

    Science.gov (United States)

    Blandino, Massimo; Reyneri, Amedeo; Vanara, Francesca; Pascale, Michelangelo; Haidukowski, Miriam; Campagna, Claudio

    2009-11-01

    The European corn borer (ECB), Ostrinia nubilalis, is the principal pest of maize in Central and South Europe. It is known to promote Fusarium verticillioides infection in maize grain, a recognized producer of fumonisins. Field experiments were performed in 2006 and 2007 in two sites in NW Italy to determine the effects of the timing of insecticide application on ECB damage, fungal ear rot and fumonisin contamination under natural conditions. Different insecticide application timings were compared, from maize flowering to approximately 15 days after the flight peak of adult ECB. At harvest, the ears were rated for incidence and severity of ECB damage, fungal ear rot symptoms and fumonisin (FB(1) + FB(2)) contamination. In all years/sites, treatments applied at the beginning of consistent ECB flight activity were most effective in controlling insect damage on ears. Fungal ear rot and fumonisin contamination were significantly affected by ECB control. The efficacy of the best timing of insecticide application in controlling fumonisin contamination was, on average, 93% compared to the untreated control. Contamination levels of these mycotoxins increased with either an earlier or later treatment. Furthermore, an earlier insecticide application showed lower fumonisin contamination than a treatment applied after the adult flight peak. Production of maize kernels and maize-based foods that do not exceed the maximum international and EU permitted levels for fumonisins could be enhanced by appropriate insecticide treatment against second generation ECB. The optimum time for insecticide application is between the beginning of consistent adult flight activity and the flight peak.

  7. Datasets for transcriptomic analyses of maize leaves in response to Asian corn borer feeding and/or jasmonic acid

    Science.gov (United States)

    Corn is one of the most widely grown crops throughout the world. However, many corn fields develop pest problems such as corn borers every year that seriously affect its yield and quality. Corn's response to initial insect damage involves a variety of changes to the levels of defensive enzymes, toxi...

  8. Wing shape and size of the western corn rootworm (Coleoptera: Chrysomelidae) is related to sex and resistance to soybean-maize crop rotation.

    Science.gov (United States)

    Mikac, K M; Douglas, J; Spencer, J L

    2013-08-01

    The western corn rootworm, Diabrotica virgifera virgifera LeConte, is a major pest of maize in the United States and more recently, Europe. Understanding the dispersal dynamics of this species will provide crucial information for its management. This study used geometric morphometric analysis of hind wing venation based on 13 landmarks in 223 specimens from nine locations in Illinois, Nebraska, Iowa, and Missouri, to assess whether wing shape and size differed between rotated and continuously grown maize where crop rotation-resistant and susceptible individuals are found, respectively. Before assessing differences between rotation-resistant and susceptible individuals, sexual dimorphism was investigated. No significant difference in wing (centroid) size was found between males and females; however, females had significantly different shaped (more elongated) wings compared with males. Wing shape and (centroid) size were significantly larger among individuals from rotated maize where crop-rotation resistance was reported; however, cross-validation of these results revealed that collection site resistance status was an only better than average predictor of shape in males and females. This study provides preliminary evidence of wing shape and size differences in D. v. virgifera from rotated versus continuous maize. Further study is needed to confirm whether wing shape and size can be used to track the movement of rotation-resistant individuals and populations as a means to better inform management strategies.

  9. Effect of Seed Blends and Soil-Insecticide on Western and Northern Corn Rootworm Emergence from mCry3A+eCry3.1Ab Bt Maize.

    Science.gov (United States)

    Frank, Daniel L; Kurtz, Ryan; Tinsley, Nicholas A; Gassmann, Aaron J; Meinke, Lance J; Moellenbeck, Daniel; Gray, Michael E; Bledsoe, Larry W; Krupke, Christian H; Estes, Ronald E; Weber, Patrick; Hibbard, Bruce E

    2015-06-01

    Seed blends containing various ratios of transgenic Bt maize (Zea mays L.) expressing the mCry3A+eCry3.1Ab proteins and non-Bt maize (near-isoline maize) were deployed alone and in combination with a soil applied pyrethroid insecticide (Force CS) to evaluate the emergence of the western corn rootworm, Diabrotica virgifera virgifera LeConte, in a total of nine field environments across the Midwestern United States in 2010 and 2011. Northern corn rootworm, Diabrotica barberi Smith & Lawrence emergence was also evaluated in four of these environments. Both western and northern corn rootworm beetle emergence from all Bt treatments was significantly reduced when compared with beetle emergence from near-isoline treatments. Averaged across all environments, western corn rootworm beetle emergence from 95:5, 90:10, and 80:20 seed blend ratios of mCry3A+eCry3.1Ab: near-isoline were 2.6-, 4.2-, and 6.7-fold greater than that from the 100:0 ratio treatment. Northern corn rootworm emergence from the same seed blend treatments resulted in 2.8-, 3.2-, and 4.2-fold more beetles than from the 100:0 treatment. The addition of Force CS (tefluthrin) significantly reduced western corn rootworm beetle emergence for each of the three treatments to which it was applied. Force CS also significantly delayed the number of days to 50% beetle emergence in western corn rootworms. Time to 50% beetle emergence in the 100% mCry3A+eCry3.1Ab treatment with Force CS was delayed 13.7 d when compared with western corn rootworm beetle emergence on near-isoline corn. These data are discussed in terms of rootworm resistance management.

  10. Maize silk antibiotic polyphenol compounds and molecular genetic improvement of resistance to corn earworm (Helicoverpa zea Boddie in sh2 sweet corn

    Directory of Open Access Journals (Sweden)

    Baozhu Guo

    2010-01-01

    Full Text Available The flavor of sh2 super-sweet corn is preferred by consumers. Unfortunately, sh2 sweet corn has little genetic variation for insect resistance. In this paper we review the functions of two loci, p1 and a1. The P1 allele has a major role in sh2 sweet corn resistance to corn earworm, an allele that was lost in historical selection because of its pleiotropic effect on undesirable cob color and silk browning. The P1 allele has significant effects on biosyntheses of silk antibiotic compounds, maysin, apimaysin, methoxymaysin, and chlorogenic acid. The effect of a1 shows gene action for lowered maysin and significant epistatic action with p1. The dominant functional allele A1 causes anthocyanin pigments in aleurone, plant, and pericarp tissues; the recessive a1 allele causes absence of pigment in these tissues. If silk browning and cob color are critical factors for maysin production but lack the customer’s preference, then separating red cob and browning silk, which are controlled by the P1 allele, may be difficult if not impossible. One high silk maysin sh2 sweet corn germ­plasm, shrunken Zapalote Chico, has been released. There is some field corn germplasm with p1-wwr alleles, but the amount of antibiotic flavones and their potential as a donor need further investigation.

  11. Mapping of QTL for resistance to the Mediterranean corn borer attack using the intermated B73 x Mo17 (IBM) population of maize.

    Science.gov (United States)

    Ordas, Bernardo; Malvar, Rosa A; Santiago, Rogelio; Sandoya, German; Romay, Maria C; Butron, Ana

    2009-11-01

    The Mediterranean corn borer or pink stem borer (MCB, Sesamia nonagrioides Lefebvre) causes important yield losses as a consequence of stalk tunneling and direct kernel damage. B73 and Mo17 are the source of the most commercial valuable maize inbred lines in temperate zones, while the intermated B73 x Mo17 (IBM) population is an invaluable source for QTL identification. However, no or few experiments have been carried out to detect QTL for corn borer resistance in the B73 x Mo17 population. The objective of this work was to locate QTL for resistance to stem tunneling and kernel damage by MCB in the IBM population. We detected a QTL for kernel damage at bin 8.05, although the effect was small and two QTL for stalk tunneling at bins 1.06 and 9.04 in which the additive effects were 4 cm, approximately. The two QTL detected for MCB resistance were close to other QTL consistently found for European corn borer (ECB, Ostrinia nubilalis Hübner) resistance, indicating mechanisms of resistance common to both pests or gene clusters controlling resistance to different plagues. The precise mapping achieved with the IBM population will facilitate the QTL pyramiding and the positional cloning of the detected QTL.

  12. Distribution and variations of three 1,4-benzoxazin-3-ones in maize induced by the Asian corn borer, Ostrinia furnacalis (Guenée).

    Science.gov (United States)

    Huang, Cuihong; Wang, Xiaoying; Wang, Rongjiang; Xue, Kun; Yan, Fengming; Xu, Chongren

    2006-01-01

    Contents of three 1,4-benzoxazin-3-ones in tissue samples from different parts (young leaf, second leaf, old leaf, stem and root) of young maize plants of 4-leaves stage, fed by the third instar larvae of the Asian corn borer, Ostrinia furnacalis (Guenée), were analyzed by high-performance liquid chromatography-mass spectroscopy (HPLC-MS). Samples were taken immediately (set A) or 48 h (set B) after larvae had fed on the second leaf for 48 h. The three 1,4-benzoxazin-3-ones investigated in our experiments were 2,4-dihydroxy-7-methoxy-1,4(2H)-benzoxazin-3-one (DIMBOA), 2,4-dihydroxy-1,4(2H)-benzoxazin-3-one (DIBOA) and 2-hydroxy-7-methoxy-1,4(2H)-benzoxazin-3-one (HMBOA). In samples of set A, the levels of DIMBOA and HMBOA were significantly lifted in the old leaf (L3) and young leaf (L1), respectively, while amounts of these two chemicals in other plant parts were not significantly different between larvae-fed plants and intact plants. Concentrations of DIBOA in each plant part remained unchanged. In samples of set B, no concentration differences for any of these three 1,4-benzoxazin-3-ones between larvae-fed plants and controls were observed in any plant part. The feeding of the Asian corn borer seems to have limited effects on induction of these three 1,4-benzoxazin-3-ones in young maize plants of the variety investigated.

  13. QTL mapping for European corn borer resistance ( Ostrinia nubilalis Hb.), agronomic and forage quality traits of testcross progenies in early-maturing European maize ( Zea mays L.) germplasm.

    Science.gov (United States)

    Papst, C; Bohn, M; Utz, H F; Melchinger, A E; Klein, D; Eder, J

    2004-05-01

    In hybrid breeding the performance of lines in hybrid combinations is more important than their performance per se. Little information is available on the correlation between individual line and testcross (TC) performances for the resistance to European corn borer (ECB, Ostrinia nubilalis Hb.) in maize ( Zea mays L.). Marker assisted selection (MAS) will be successful only if quantitative trait loci (QTL) found in F(2) derived lines for ECB resistance are still expressed in hybrid combinations. The objectives of our study were: (1) to identify and characterize QTL for ECB resistance as well as agronomic and forage quality traits in a population of testcrossed F(2:3) families; (2) to evaluate the consistency of QTL for per se and TC performances; and (3) to determine the association between per se and TC performances of F(2:3) lines for these traits. Two hundred and four F(2:3) lines were derived from the cross between maize lines D06 (resistant) and D408 (susceptible). These lines were crossed to D171 and the TC progenies were evaluated for ECB resistance and agronomic performance in two locations in 2000 and 2001. Using these TC progenies, six QTL for stalk damage rating (SDR) were found. These QTL explained 27.4% of the genotypic variance in a simultaneous fit. Three QTL for SDR were detected consistently for per se and TC performance. Phenotypic and genotypic correlations were low for per se and TC performance for SDR. Correlations between SDR and quality traits were not significant. Based on these results, we conclude that MAS will not be an efficient method for improving SDR. However, new molecular tools might provide the opportunity to use QTL data as a first step to identify genes involved in ECB resistance. Efficient MAS procedures might then be based on markers designed to trace and to combine specific genes and their alleles in elite maize breeding germplasm.

  14. Utilization of Bioslurry on Maize Hydroponic Fodder as a Corn Silage Supplement on Nutrient Digestibility and Milk Production of Dairy Cows

    Directory of Open Access Journals (Sweden)

    H. D. Nugroho

    2015-04-01

    Full Text Available The research was conducted to study the effect of addition of 7% DM maize hydroponic fodder (MHF in corn silage on digestibility and milk production of dairy cows. The experiment used a completely randomized block design with two treatments, and four replications. The treatments were dairy cows fed with grass (Pennisetum purpureum, corn silage, and concentrate (R0, and dairy cows fed with grass (P. purpureum, corn silage, concentrate, and MHF (R1. This research used eight dairy cows with initial average milk production of 13.01±2.96 L/d. MHF was produced in a hydroponic system using bioslurry as a fertilizer enriched with mineral fertilizer. Variables observed were chemical composition of bioslurry, nutrient content of ration, daily dry matter intake, nutrient digestibility, Total Digestible Nutrient (TDN, and Digestible Energy (DE. Data were analyzed with ANOVA, except for milk production using ANCOVA. Supplementation of MHF resulted a higher total dry matter intake on R1 than R0 (P<0.05, 12.99±0.063 kg/head/d, and 11.98±0.295 kg/head/d, respectively. The digestibility of nutrients were not affected by the addition of MHF. Energy consumption in R1 was also higher than R0 (P<0.05, 49.95±0.36 Mkal/kg, and 46.11±0.54 Mkal/kg, respectively. Supplementation of MHF also increased nitrogen consumption, R1 was higher than R0 (P<0.05, 318.3±2.3 g/head/d, and 295.9±3.5 g/head/d, respectively, and could maintain the persistency of milk production at the end of lactation. It can be concluded that supplementation of MHF in corn silage can increase dry matter intake, energy consumption, and nitrogen consumption, also can maintain nutrient digestibility and maintain persistency of milk production during late lactation of dairy cows.

  15. Molecular confirmation of Maize rayado fino virus as the Brazilian corn streak virus Confirmação molecular do 'Maize rayado fino virus' como vírus da estria do milho

    Directory of Open Access Journals (Sweden)

    Rosemarie Wahnbaeck Hammond

    2005-12-01

    Full Text Available Maize rayado fino virus (MRFV, present in various countries in Latin America, has shown similarities to corn streak virus that occurs in Brazil, regarding pathogenic, serological and histological characteristics. In the current report both virus were molecularly compared to confirm the similarities between them. MRFV was identified by nucleic acid hybridization in samples of maize tissues exhibiting symptoms of "corn stunt" disease, collected from two Brazilian States - São Paulo and Minas Gerais. The coat protein gene and 3'non-translated region of MRFV were amplified from infected tissues by reverse transcription-polymerase chain reaction (RT-PCR using MRFV-specific primers, and were characterized by nucleotide sequence and phylogenetic analysis of the cloned PCR products. Phylogenetic analysis of the relationships between the Brazilian isolates and isolates obtained from Latin America and the United States reveals a close relationship to isolates from Brazil, Peru and Bolivia. Results support the proposal that the Brazilian corn streak virus be regarded as an isolate of MRFV and provide evidence for the presence of MRFV in "corn stunt' disease in Brazil.O vírus do rayado fino do milho (MRFV, presente em vários países da América Latina, tem mostrado semelhança ao vírus da risca do milho que ocorre no Brasil, em relação a características patogênicas, sorológicas e histológicas. No presente trabalho, ambos os vírus foram comparados molecularmente, visando confirmar a similaridade entre os mesmos. O MRFV foi identificado por hibridização de ácido nucléico em amostras de tecido que apresentavam sintomas de enfezamento, coletadas nos estados de São Paulo e Minas Gerais. O gene da capa protéica e a região 3' não traduzida do MRFV foram amplificados, a partir de tecidos infectados, através da transcrição reversa por reação em cadeia da polimerase (RT-PCR, usando-se os iniciadores específicos para o vírus, obtendo-se a seq

  16. The effect of organic acid mixture and bacterial inoculant on fermentation in laboratory silos of climper high moisture maize grain corn

    Directory of Open Access Journals (Sweden)

    Václav Pyrochta

    2005-01-01

    Full Text Available In the experiment, the effect of chemical (A and biological (B additiva on the fermentation quality of climper high moisture maize grain corn was examined, compared with the untreated control (K. The chemical means contained propionic, formic and benzoic acids and ammonium formate as effective substances. As effective substances of bacterial water–soluble inoculants, selected were bacterial strains of Propionibacterium shermanii JS and Lactobacillus casei LC-705. Both conservative preservatives were added equally to the ensilaged material. The addition of chemical additivum under conditions of our experiment increased statistically significantly (P<0.01 the contents of acetic acid (7.66±0.95 g/kg DM, ethanol (11.22±0.65 g/kg DM and pH values (4.33±0.02 in experimental silages. Simultaneously, a statistically significant (P<0.01 inhibition of lactic acid formation (15.17±2.75 g/kg DM and of total content of all fermentation acids (22.33±2.38 g/kg DM occurred. The bacterial inoculant increased significantly (P<0.05 the content of lactic acid (26.78±2.63 g/kg DM and the total acid content (32.87±2.88 g/kg DM in inoculated silages. The inoculation positive effect was demonstrated highly significantly (P<0.01 in reduction of ethanol amount (2.14±0.40 g/kg DM and of totat acidification. The pH value (4.21±0.02 was significantly lower (P<0.05 than that in the control silage (4.24±0.02. The fermentation characteristics in the inoculated silages by us were more favourable. The addition of organic acid mixture in the used concentration of 3.5 L/t did not confirm the positive effect on climper maize grain corn quality as expected.

  17. 2009年甘肃省玉米穗腐病、茎基腐病的发生危害%Occurrence of maize kernel rot and corn stalk rot in Gansu in 2009

    Institute of Scientific and Technical Information of China (English)

    郭满库; 王晓鸣; 何苏琴; 刘永刚; 金社林; 曹世勤; 魏宏玉

    2011-01-01

    The occurrence and damage of maize kernel rot and corn stalk rot were comprehensively investigated in 21 counties of 9 cities in Gansu in 2009. The representative samples of the diseases were collected and the pathogens were isolated on PSA. The results showed that the disease of maize kernel rot and corn stalk rot seriously occurred in main maize-producing areas of the whole province. The rate of diseased fields reached to 100%, and the rate of diseased spikes and infected plants were 63.57% and 34.47%, respectively. The results of isolation showed that the main pathogens of maize kernel rot and corn stalk rot were Fusarium graminearum, F. Culmorum and F. Verticillioides (F. Moniliforme), but the dominant species of pathogens in different regions were different.%2009年对甘肃省9个市的21个县、区玉米穗腐病、茎基腐病的发生危害情况进行了全面调查,并采集病害标样,进行病原分离.调查结果表明,玉米穗腐病、茎基腐病在全省各玉米种植区均普遍严重发生,病田率100%,病穗率和病株率平均为63.57%和34.47.初步分离结果表明,全省玉米穗腐病、茎基腐病的主要致病菌为禾谷镰刀菌、黄色镰刀菌和轮枝镰孢菌,但在地区间的分布有明显差异.

  18. Mortality of western corn rootworm larvae on MIR604 transgenic maize roots: field survivorship has no impact on survivorship of F1 progeny on MIR604

    Science.gov (United States)

    Mortality of western corn rootworm (Diabrotica virgifera virgifera LeConte) larvae due to MIR604 transgenic corn (Zea mays L.) expressing the modified Cry3A (mCry3A) protein relative to survivorship on corn with the same genetic background without the gene (isoline corn) was evaluated at three Misso...

  19. ZEA MAIZE: A MODERN CRAZE

    Directory of Open Access Journals (Sweden)

    Dhamija Isha

    2013-06-01

    Full Text Available Zea stands for ‘sustaining life’ and Mays stands for ‘life giver’. Zea mays is one of the oldest and most dynamic crop species, which has gained popularity in modern world too, due to its applications in diverse dishes. Corn is produced in every continent of the world with the exception of Antarctica. It is an annual monoecious sunny plant, surviving perfectly in nutrient rich, well-drained soil. Each and every part of the corn, from husk to corn silk is beneficial for the society. There are more than 3,500 different uses for corn products. Corn does much more than feed people and livestock. The plant contains alkaloids, flavonoids, saponins, maizenic acid, vitamins B1, K and minerals like potassium, phosphorous and zinc. Traditionally, Maize is used as an analgesic, anti-diarrheal, anti-prostatitic, anti-lithiasis, anti-tumor, anti-hypertensive, anti-diabetic, anti-hyperlipidemic, anti-inflammatory and anti-oxidant. In this review article, we have narrated miscellaneous uses of corn varieties and described the pharmacological activities, phytoconstituents, nutritional value and traditional uses of maize. The maize has assorted uses like culinary, medicinal and industrial. Corn dishes like corn-meal, corn-flakes, popcorn, “makki ki roti” and corn soup highlight its dominance all over the world. Therefore, maize has become a craze among modern youth.

  20. Types of Maize Virus Diseases and Progress in Virus Identification Techniques in China

    Institute of Scientific and Technical Information of China (English)

    Cui Yu; Zhang Ai-hong; Ren Ai-jun; Miao Hong-qin

    2014-01-01

    There are a total of more than 40 reported maize viral diseases worldwide. Five of them have reportedly occurred in China. They are maize rough dwarf disease, maize dwarf mosaic disease, maize streak dwarf disease, maize crimson leaf disease, maize wallaby ear disease and corn lethal necrosis disease. This paper reviewed their occurrence and distribution as well as virus identification techniques in order to provide a basis for virus identification and diagnosis in corn production.

  1. Landscape refuges delay resistance of the European corn borer to Bt-maize: a demo-genetic dynamic model.

    Science.gov (United States)

    Tyutyunov, Yuri; Zhadanovskaya, Ekaterina; Bourguet, Denis; Arditi, Roger

    2008-08-01

    We constructed a reaction-diffusion model of the development of resistance to transgenic insecticidal Bt crops in pest populations. Kostitzin's demo-genetic model describes local interactions between three competing pest genotypes with alleles conferring resistance or susceptibility to transgenic plants, the spatial spread of insects being modelled by diffusion. This new approach makes it possible to combine a spatial demographic model of population dynamics with classical genetic theory. We used this model to examine the effects of pest dispersal and of the size and shape of the refuge on the efficiency of the "high-dose/refuge" strategy, which was designed to prevent the development of resistance in populations of insect pests, such as the European corn borer, Ostrinia nubilalis Hübner (Lepidoptera, Crambidae). We found that, with realistic combinations of refuge size and pest dispersal, the development of resistance could be considerably delayed. With a small to medium-sized farming area, contiguous refuge plots are more efficient than a larger number of smaller refuge patches. We also show that the formal coupling of classical Fisher-Haldane-Wright population genetics equations with diffusion terms inaccurately describes the development of resistance in a spatially heterogeneous pest population, notably overestimating the speed with which Bt resistance is selected in populations of pests targeted by Bt crops.

  2. DISEÑO MECANICO DE UN PROTOTIPO DE SEMBRADORA DE MAIZ MECHANICAL DESIGN OF A PROTOTYPE CORN SOWER

    Directory of Open Access Journals (Sweden)

    Salvador Barragán G

    2006-08-01

    Full Text Available La agricultura mexicana es un sector estratégico para el desarrollo nacional. El maíz desempeña un papel importante en la industria, ya que se procesa derivando una gran cantidad de productos y subproductos. En la actualidad hay campesinos que siembran en terrenos irregulares como cerros o terrenos de superficie inclinada; este tipo de siembra la mayoría de las veces es agricultura de subsistencia, pero no por eso menos importante que la comercial. La siembra en este tipo de terrenos se realiza por medio de herramientas rústicas debido al difícil acceso de maquinaria moderna y los pocos recursos económicos para conseguirla; este tipo de herramienta representa un gran esfuerzo físico y jornadas muy largas de trabajo para el campesino. En el presente proyecto se propone una máquina para sembrar maíz, la cual evitaría los problemas anteriormente expuestos, además de realizar la actividad en menor tiempo y de manera económica, rentable y segura.Mexican agriculture is a strategic sector for national development. Corn has an important role in the industry due to the fact that it is processed into a great variety of products and by-products. There are some agricultural workers that sow in irregular grounds such as hills and sloping surfaces. This kind of sowing is often for their own consumption, but it is not less important than the industrial counterpart. Sowing in this type of grounds is done with rustic tools owing to lack of access to modern equipment, due to its cost and other reasons. The use of rustic tools is hard work and time-consuming for the workers. In this project, a proposal to make this job easier on sloping grounds is presented. This proposal consists of a machine that reduces the physical exertion and time required of the workers, by increasing the sowing speed, making the operation less expensive, safer and more efficient.

  3. 黑龙江不同积温带玉米品种抗螟性分析%Maize Resistance to Corn Borer in Different Temperature Zones of Heilongjiang Province

    Institute of Scientific and Technical Information of China (English)

    张海燕; 王丽艳; 程晓娟; 赵长江; 林志伟; 孙强; 吴金花; 金永玲

    2013-01-01

      通过对百株折茎率、折雄率、茎秆钻蛀率及越冬幼虫数量和体重等指标分析,分析7个黑龙江省不同积温带玉米品种的抗虫性。研究结果表明,供试的7个不同积温带品种表现高抗的品种为垦单5和高油4515,中抗品种郑单958和兴垦3;高感品种为哲单37和绥玉19,中感品种德美亚1号。%Based on the analysis of the stalk broken rate, the tassel broken rate, the bored stems rate, the number of over-wintering larvaes and the weight, maize resistance to corn borer was studied in 7 temperature zones of Hei-longjiang province. The results showed that there were 7 maize varieties having resistance to corn borer, including 2 high resistant varieties, Kendan5 and Gaoyou4515;2 middle-resistant varieties, Zhengdan958 and Xingken3;2 high sensitive varieties, Zhedan37 and Suiyu19;one middle-sensitive, Demeiya1.

  4. 玉米种质资源对六种重要病虫害的抗性鉴定与评价%Screening and Evaluation of Maize Germplasm for Resistance to Five Diseases and Asian Corn Borer

    Institute of Scientific and Technical Information of China (English)

    段灿星; 朱振东; 武小菲; 杨知还; 王晓鸣

    2012-01-01

    在2003-2005年间,对604份玉米种质进行了抗弯孢菌叶斑病和玉米螟鉴定,筛选出抗弯孢菌叶斑病的材料93份,抗玉米螟材料22份.2006-2009年间,对836份玉米种质进行了抗大斑病、茎腐病、穗腐病和瘤黑粉病的鉴定与评价,筛选出一批高抗和多抗的资源.在836份资源中,对大斑病1、2和N号3个生理小种具有抗性的材料均为50%左右;抗茎腐病材料为41.3%,高抗和抗性种质分别为264和81份;穗腐病高抗和抗性种质分别为5和171份,占比为21.1%;瘤黑粉病高抗和抗性种质各261和14份,占总鉴定材料的32.9%.上述结果表明抗大斑病、茎腐病和瘤黑粉病的种质资源较为丰富.通过对抗性结果进行对比分析,发现不同生态区玉米种质的抗性强弱以及抗性多样性存在明显差异,黑龙江和内蒙古的种质对病虫害的抗性强弱及多样性程度明显高于四川种质.此外,玉米自交系对病虫害的抗性强弱以及多抗性程度高于农家种.%Among 604maize accessions screened in 2003 -2005,93 were resistant to Curvularia leaf spot and 22 were resistant to Asian corn borer. From 2006 to 2009 ,836 maize accessions were identified and evaluated for resistance to northern corn leaf blight, Pythium stalk rot, Fusarium ear rot and common smut and some of them were highly resistant and multiplely resistant. Approximately 50% of maize accessions with resistance to northern corn leaf blight racel ,2 and N were screened,respectively. 41. 3% of accessions were resistant to Pythium stalk rot,including 264 highly resistant and 81 resistant. 5 and 171 accessions were highly resistant and resistant to Fusarium ear rot,respectively,accounting for 21. 1% of total accessions screened. 32. 9% of accessions with different resistance to common smut was screened,261 highly resistant and 14 resistant lines were discovered. Based on the above data, we thought there were relatively abundant maize germplasm with

  5. The lost p1 allele in sh2 sweet corn: Quantitative effects of p1 and a1 genes on the concentrations of maysin, apimaysin, methoxymaysin, and chlorogenic acid in maize silk, and the antibiotic activity against corn earworm

    Science.gov (United States)

    The flavor of sh2 super-sweet corn is preferred by consumers. Unfortunately, sh2 sweet corn has very little genetic variation for resistance to insects. This presentation will review and summarize the studies of the functions of two loci, p1 and a1. The P1 allele can have a major role in the resista...

  6. Conservação pós-colheita de espigas de milho verde minimamente processado sob diferentes temperaturas Post-harvest conservation of fresh-cut corn on the cob (Zea maiz L. under different temperatures

    Directory of Open Access Journals (Sweden)

    Alexandra Mara Goulart Nunes Mamede

    2009-02-01

    Full Text Available Objetivou-se, neste trabalho, avaliar o efeito de três temperaturas (5ºC, 8ºC e 11ºC, na qualidade de híbridos de milho verde (Zea maiz L. com endosperma normal, minimamente processado, durante oito dias de armazenamento, com avaliações a cada dois dias. Foram utilizadas espigas de dois híbridos, sendo um comercial da Sementes Agroceres (Ag 1051 e outro do programa de melhoramento da Embrapa Milho e Sorgo (Embrapa HT1. A temperatura de 5ºC foi a que melhor preservou a qualidade das espigas dos híbridos de milho verde estudados, por proporcionar perda de massa reduzida e manutenção dos teores de sólidos solúveis, frutose e glicose. O híbrido Ag 1051 apresentou menor perda de massa, maiores valores de umidade e maior teor de frutose. O Embrapa HT1 apresentou maiores valores iniciais e finais para os teores de sólidos solúveis e maior valor para a coloração b*. O valor L*, que indica quãoclaro e escuro é produto, também diminuiu ao longo do armazenamento, indicando escurecimento das espigas ao longo do armazenamento.This work had the aim of evaluating the effect of three temperatures (5ºC, 8ºC and 11ºC on the quality of fresh-cut corn on the cob (Zea maiz L. with normal endosperm, for 8 days, with analyses taken every two days. Corn on the cob of two hybrids was used, a commercial common type from Sementes Agroceres (Ag 1051 and the other from the breeding program Embrapa Milho e Sorgo (Embrapa HT1. The temperature of 5ºC was more efficient to preserve the quality of normal corn by promoting reduced mass loss and higher contents of soluble solids, fructose and glucose. The hybrid Ag 1051 presented lower mass loss, higher contents of moisture and higher content of fructose. The hybrid Embrapa HT1 presented higher initial and final values for soluble solids and higher b* value. The L* value also decreased along storage, indicating browning of the corns along the storage.

  7. Effects of Maize Planting Density on Occurrence of Asian Corn Borer and Trichogramma Parasitism%玉米种植密度对玉米螟发生及赤眼蜂寄生的影响

    Institute of Scientific and Technical Information of China (English)

    李丽娟; 鲁新; 周淑香; 张国红; 丁岩; 刘宏伟

    2013-01-01

    Asian corn bore(ACB) and Trichogramma were investigated with diverse maize planting density in a maize field. The results showed that the 100-maize plants egg masses amount and eggs number were relatively high of the 1st and 2nd generation ACB with low planting density. The corn was damaged serious by the 1st-generation ACB in 45 000 plants/ha maize field. In addition, The correlation analysis showed that egg masses amount, eggs number, bored-hole number, hole pass length, alive worm amount per 100-maize plants and the parasitism rate of ACB egg masses were negatively correlated with planting density. Healthy plants number was positively correlated with planting density. The broken-up ear stem rate, the broken-ear handle rate, the broken-tassel rate increased a little as maize planting density increases. The planting density had almost no effect on natural parasitism rate of Trichogramma. There were slightly higher the egg masses parasitism rate(figure 44.24%) and the eggs parasitism rate(figure 39.00%) of the 1st-generation ACB with 30 000 plants/ha than other planting density. Both were very high the egg masses par-asitism rate and the eggs parasitism rate of the 2nd-generation ACB with diverse planting density. The average values were 84.59%and 80.84%.%通过对不同种植密度玉米田内玉米螟、赤眼蜂调查,初步认为,在低种植密度下一、二代玉米螟百株落卵块数、落卵粒数较高,种植密度为45000株/hm2玉米田的一代玉米螟危害较重。相关分析表明,一、二代百株玉米的落卵块数、落卵粒数、蛀孔数、隧道长度、活虫数及一代卵块寄生率等与种植密度呈负相关;健株数与种植密度呈正相关;穗上折率、穗柄折率、雄穗折率随种植密度增加折率略有增加。玉米不同种植密度对赤眼蜂的自然寄生率影响较小,30000株/hm2种植密度一代玉米螟的卵块寄生率和卵粒寄生率略高,为44.24%和39.00%;二代玉米螟

  8. Mortality of western corn rootworm larvae on MIR604 transgenic maize roots: field survivorship has no significant impact on survivorship of F1 progeny on MIR604.

    Science.gov (United States)

    Hibbard, Bruce E; Clark, Thomas L; Ellersieck, Mark R; Meihls, Lisa N; El Khishen, Ahmed A; Kaster, Von; Steiner, Henry-York; Kurtz, Ryan

    2010-12-01

    Mortality of western corn rootworm, Diabrotica virgifera virgifera LeConte, larvae due to MIR604 transgenic corn, Zea mays L., expressing the modified Cry3A (mCry3A) protein relative to survivorship on corn with the same genetic background without the gene (isoline corn) was evaluated at three Missouri sites in both 2005 and 2006. We made these comparisons by using wild-type western corn rootworm at three different egg densities (6,000, 3,000, and 1,500 eggs per m) so that the role of density-dependent mortality would be known. The mortality due to the mCry3A protein was 94.88% when averaged across all environments and both years. Fifty percent emergence of beetles was delayed approximately 5.5 d. Beetles were kept alive and their progeny evaluated on MIR604 and isoline corn in the greenhouse to determine whether survivorship on MIR604 in the field for one generation increased survivorship on MIR604 in the greenhouse in the subsequent generation. There was no significant difference in survivorship on MIR604 in greenhouse assays between larvae whose parents survived isoline and larvae whose parents survived MIR604 in the field the previous generation, indicating that many susceptible beetles survived MIR604 in the field the previous season along with any potentially resistant beetles. The data are discussed in terms of rootworm insect resistance management.

  9. Optimization of Ultrasonic-assisted Extraction Parameters of Maize Yellow Pigment from Corn%超声波强化提取玉米黄色素工艺条件优化

    Institute of Scientific and Technical Information of China (English)

    李万林; 钟姣姣; 刘彩芬; 赵红红; 李波

    2014-01-01

    以玉米皮为原料,利用超声波技术,以乙醇为提取剂从玉米皮中提取玉米黄色素,探讨了料液比、乙醇体积百分数、提取温度和提取时间对玉米黄色素提取率的影响。结果表明:最佳工艺条件为以乙醇作为提取剂,体积分数为60%,玉米皮与乙醇的料液比为1:15,提取温度为50℃,超声波处理时间为30 min,乙醇提取率可达到6.274%。%The maize yellow pigment was extracted from corn barn through ultrasonic-assisted extraction technology using ethanol as an extracting agent. The effects of parameters such as material-to-liquid ratio, volume fraction of ethanol, extracting temperature and time on the extraction rate of yellow pigment were investigated. The results showed that the extraction rate was up to 6.274% under the optimum extraction condition, e.g. volume percentage of ethanol 60%, corn barn to ethanol ratio of 1:15, extraction temperature 50℃and extraction time of 30 min.

  10. Study of southern corn

    Directory of Open Access Journals (Sweden)

    Samavia Mubeen

    2017-07-01

    Full Text Available Southern corn leaf blight is considered the most devastating disease of maize crop, which causes noticeable reduction in crop yield. Inbred lines are useful because they are genotyped, multiple time phenotyping is possible, and genetic uniformity, genetic stability and its vigor make inbred lines suitable to study in diversified environment. In present investigation, 12 maize genotypes viz: NC-2703 (hybrid, NC-2003 (hybrid, SP-3 (inbred line, NCML-73 (inbred line, NRL-6 (inbred line, NRL-4 (inbred line, Soan-3 (variety, Rakaposhi (variety, Margala (variety, EV-1097 (variety, Local-Y (variety, Local-W (variety were tested against southern corn leaf blight under laboratory and field conditions. According to disease severity scale (0–5 inbreds SP-3 and NCML-73 were found highly resistant; Local-W moderately resistance and rest of the genotypes were least resistance in in vitro analysis. In field screening, Margala, NRL-4, EV-1097 showed maximum resistance followed by moderately resistant SP-3, NCML-73, NC-2703, NRL-6 and Local-Y maize genotypes. NC-2003, Rakaposhi and Soan-3 showed least resistance during field evaluation. Cochliobolus heterostrophus showed considerable effects on yield of crop. Significant difference was found in grain yield, plant height, ear height and ear weight while ear placement, ear per plant and infected ear data were non-significant. The results clearly showed the effect on maize genotypes and its yield.

  11. Oviposition behaviour and egg distribution of the European corn borer, Ostrinia nubilalis, on maize, and its effect on host finding by Trichogramma egg parasitoids

    NARCIS (Netherlands)

    Suverkropp, B.P.; Dutton, A.; Bigler, F.; Lenteren, van J.C.

    2008-01-01

    Oviposition behaviour and egg distribution of Ostrinia nubilalis is reviewed based on published information and new research. The position of egg masses of O. nubilalis on maize plants and leaves were sampled in the field. Most egg masses were found on the lower leaf side, on the middle part of the

  12. Development of Maize Harvesting Machines and New Corn Header%玉米收获机及新型玉米收割台的发展研究

    Institute of Scientific and Technical Information of China (English)

    李文刚

    2013-01-01

    This paper describes the present situation and the progress in domestic corn harvesters. The corn harvest mechanization level in China is still relatively low. In view of the technical problems of current corn harvesting machinery in China, as well as China's na-tional conditions, the author expounds on the significance of the research on and the development of new corn headers that are likely to adapt to different requirements of different areas across China.%概述国内外玉米收获机的发展现状,结合目前我国玉米收获机械化程度较低、玉米收获机械存在技术问题的国情,阐述研究开发适应我国不同地区玉米收获农艺技术要求的新型玉米收割台的重要意义。

  13. Bt maize and integrated pest management--a European perspective.

    Science.gov (United States)

    Meissle, Michael; Romeis, Jörg; Bigler, Franz

    2011-09-01

    The European corn borer (Ostrinia nubilalis), the Mediterranean corn borer (Sesamia nonagrioides) and the western corn rootworm (Diabrotica virgifera virgifera) are the main arthropod pests in European maize production. Practised pest control includes chemical control, biological control and cultural control such as ploughing and crop rotation. A pest control option that is available since 1996 is maize varieties that are genetically engineered (GE) to produce insecticidal compounds. GE maize varieties available today express one or several genes from Bacillus thuringiensis (Bt) that target corn borers or corn rootworms. Incentives to growing Bt maize are simplified farm operations, high pest control efficiency, improved grain quality and ecological benefits. Limitations include the risk of resistance evolution in target pest populations, risk of secondary pest outbreaks and increased administration to comply with licence agreements. Growers willing to plant Bt maize in the European Union (EU) often face the problem that authorisation is denied. Only one Bt maize transformation event (MON810) is currently authorised for commercial cultivation, and some national authorities have banned cultivation. Spain is the only EU member state where Bt maize adoption levels are currently delivering farm income gains near full potential levels. In an integrated pest management (IPM) context, Bt maize can be regarded as a preventive (host plant resistance) or a responsive pest control measure. In any case, Bt maize is a highly specific tool that efficiently controls the main pests and allows combination with other preventive or responsive measures to solve other agricultural problems including those with secondary pests.

  14. Assessment of fitness costs in Cry3Bb1 resistant and susceptible western corn rootworm (Coleoptera:Chrysomelidae) laboratory colonies

    Science.gov (United States)

    Maize production in the United States is dominated by plants genetically modified with transgenes from Bacillus thuringiensis (Bt). Varieties of Bt maize expressing Cry3Bb endotoxins that specifically target corn rootworms (genus Diabrotica) have proven highly efficacious. Howeve...

  15. Responses by earthworms to reduced tillage in herbicide tolerant maize and Bt maize cropping systems

    DEFF Research Database (Denmark)

    Krogh, P. H.; Griffiths, B.; Demsar, D.

    2007-01-01

    -toxin producing transgenic maize line MON810 was studied for 1 year. At a Danish study site, Foulum (Jutland), one year of Bt corn was followed by 2 years of herbicide tolerant corn. At the French study site the most prominent effects observed were due to the tillage method where RT significantly reduced...

  16. Enhanced Resistance of Snowdrop Lectin (Galanthus nivalis L. Agglutinin)-Expressing Maize to Asian Corn Borer (Ostrinia furnacalis Guenée)

    Institute of Scientific and Technical Information of China (English)

    Zhao-Yu WANG; Xiao-Fen SUN; Fei WANG; Ke-Xuan TANG; Ju-Ren ZHANG

    2005-01-01

    In order to enhance the resistance to pests, transgenic maize (Zea mays L.) plants from elite inbred lines containing the gene encoding snowdrop lectin (Galanthus nivalis L. agglutinin; GNA) under control of a phloem-specific promoter were generated through the Agrobacterium tumefaciens-mediated also studied. Thirty-six independently derived plants were subjected to molecular analyses. The level of GNA expression at 0.13%-0.28% of total soluble protein was observed in different transgenic plants. The progeny of three GNA-expressing independent transformants that were derived separately from the elite inbred lines DH4866, DH9942, and 8902, were selected for examination of resistance to ACB. These plants synthesized GNA at levels above 0.24% total soluble protein and enhanced resistance to ACB was demonstrated by exposing the plants to insects under greenhouse conditions. Semi-artificial diet bioassays also showed the toxic effect of GNA on ACB. Field evaluation of the transgenic plants supported the results from the artificial trial. In the present study, we have obtained new insect-resistant maize material for further breeding work and have found that GNA-expressing plants not only gained significant resistance to homopterans, but also showed toxicity to ACB, which is a type of Lepidoptera.

  17. CORN FLAVOR

    Science.gov (United States)

    Corn is a large part of the modern diet through sweeteners, oil, processed foods, and animal-derived foods. In addition, corn is eaten directly in bread and cereal-type foods, snack foods, and foods made from masa flour. Corn gluten meal is a byproduct of grain processed by wet milling. Although pri...

  18. Global maize production, utilization, and consumption.

    Science.gov (United States)

    Ranum, Peter; Peña-Rosas, Juan Pablo; Garcia-Casal, Maria Nieves

    2014-04-01

    Maize (Zea mays), also called corn, is believed to have originated in central Mexico 7000 years ago from a wild grass, and Native Americans transformed maize into a better source of food. Maize contains approximately 72% starch, 10% protein, and 4% fat, supplying an energy density of 365 Kcal/100 g and is grown throughout the world, with the United States, China, and Brazil being the top three maize-producing countries in the world, producing approximately 563 of the 717 million metric tons/year. Maize can be processed into a variety of food and industrial products, including starch, sweeteners, oil, beverages, glue, industrial alcohol, and fuel ethanol. In the last 10 years, the use of maize for fuel production significantly increased, accounting for approximately 40% of the maize production in the United States. As the ethanol industry absorbs a larger share of the maize crop, higher prices for maize will intensify demand competition and could affect maize prices for animal and human consumption. Low production costs, along with the high consumption of maize flour and cornmeal, especially where micronutrient deficiencies are common public health problems, make this food staple an ideal food vehicle for fortification.

  19. Corn in consortium with forages

    Directory of Open Access Journals (Sweden)

    Cássia Maria de Paula Garcia

    2013-12-01

    Full Text Available The basic premises for sustainable agricultural development with focus on rural producers are reducing the costs of production and aggregation of values through the use crop-livestock system (CLS throughout the year. The CLS is based on the consortium of grain crops, especially corn with tropical forages, mainly of the genus Panicum and Urochloa. The study aimed to evaluate the grain yield of irrigated corn crop intercropped with forage of the genus Panicum and Urochloa. The experiment was conducted at the Fazenda de Ensino, Pesquisa e Extensão – FEPE  of the Faculdade de Engenharia - UNESP, Ilha Solteira in an Oxisol in savannah conditions and in the autumn winter of 2009. The experimental area was irrigated by a center pivot and had a history of no-tillage system for 8 years. The corn hybrid used was simple DKB 390 YG at distances of 0.90 m. The seeds of grasses were sown in 0.34 m spacing in the amount of 5 kg ha-1, they were mixed with fertilizer minutes before sowing  and placed in a compartment fertilizer seeder and fertilizers were mechanically deposited in the soil at a depth of 0.03 m. The experimental design used was a randomized block with four replications and five treatments: Panicum maximum cv. Tanzania sown during the nitrogen fertilization (CTD of the corn; Panicum maximum cv. Mombaça sown during the nitrogen fertilization (CMD of the corn; Urochloa brizantha cv. Xaraés sown during the occasion of nitrogen fertilization (CBD of the corn; Urochloa ruziziensis cv. Comumsown during the nitrogen fertilization (CRD of the corn and single corn (control. The production components of corn: plant population per hectare (PlPo, number of ears per hectare (NE ha-1, number of rows per ear (NRE, number of kernels per row on the cob (NKR, number of grain in the ear (NGE and mass of 100 grains (M100G were not influenced by consortium with forage. Comparing grain yield (GY single corn and maize intercropped with forage of the genus Panicum

  20. Efeito de esterco bovino sobre os rendimentos de espigas verdes e de grãos de milho The effect of cattle manure on yield of green corn ears and maize grains

    Directory of Open Access Journals (Sweden)

    Jaeveson da Silva

    2004-06-01

    Full Text Available Avaliaram-se os efeitos de doses de esterco bovino (0; 8; 16; 24; 32 e 40 t ha-1 sobre os rendimentos de espigas verdes e de grãos de duas cultivares de milho (Centralmex e AG-9012. O trabalho foi realizado em Mossoró (RN, de setembro a dezembro/99, com irrigação por aspersão. Utilizou-se esquema de parcelas subdivididas no delineamento de blocos completos casualizados com três repetições. As doses de esterco foram aplicadas às parcelas e as cultivares, às subparcelas. O rendimento de milho verde foi avaliado pelo número e peso totais de espigas verdes empalhadas e pelo número e peso de espigas comercializáveis, empalhadas e despalhadas. O rendimento de grãos foi avaliado pelo peso dos grãos corrigido para 15,5% de umidade. Análises do solo, realizadas aos 120 dias após o plantio, constataram que o esterco aumentou a retenção e a disponibilidade de água e os teores de fósforo, potássio e sódio, na camada do solo de 20-40 cm, mas não influenciou o pH e os teores de cálcio, soma de bases e de matéria orgânica. Tanto o rendimento de espigas verdes como o rendimento de grãos aumentaram com o aumento da dose de esterco, exceto o número e o peso totais de espigas verdes da cultivar Centralmex. A cultivar AG-9012 foi superior à cultivar Centralmex quanto aos rendimentos de espigas verdes e de grãos. A receita líquida, calculada com a comercialização de espigas empalhadas comercializáveis, foi maior na ausência de esterco para a cultivar AG-9012 e com a aplicação de 8 t ha-1para a Centralmex.The effect of different levels of cattle manure (0; 8; 16; 24; 32 and 40 t ha-1 on yield of green corn ears and grains of two maize cultivars was evaluated (Centralmex and AG-9012. The study was carried out at Mossoró, Rio Grande do Norte State, Brazil, from September to Dezember 1999 using sprinkler irrigation. The experimental design was complete random blocks arranged in splitplot with three replications. The manure was

  1. Selection and breeding of corn to enhance associative bacterial nitrogen fixation

    Energy Technology Data Exchange (ETDEWEB)

    Ela, S.W.; Anderson, M.A.; Brill, W.J.

    1980-01-01

    We have increased, through screening and breeding, the ability of corn (maize, Zea mays L.) to support bacterial nitrogen fixation in or on corn roots. Isotopic N fixed from /sup 15/N/sub 2/ was found on the roots. Even though the nitrogen-fixing association depends on germ plasm from tropical corn, the activity can be bred into corn currently used in midwestern United States agriculture.

  2. Economic efficiency of the maize grain

    Directory of Open Access Journals (Sweden)

    Ana Mariana Dincu

    2014-11-01

    Full Text Available In this work, was calculated and the level of profitability for several levels of production for grain maize cultivation. We chose corn because it is one of the most important forage crops, we could say even the largest, occupying third place among cultivated plants worldwide. Along with wheat and barley, the food is the biggest part of the population in the world, directly or converted to animal products. Maize can be used in animal feed in various forms. The most used is corn grain, which is characterized by a very high nutritional value, this product is properly regarded as a feed concentrate. Culture of maize have been designed two levels of production: 4000 kg / ha and 6000 kg / ha.

  3. Fungal growth and fusarium mycotoxin content in isogenic traditional maize and genetically modified maize grown in France and Spain.

    Science.gov (United States)

    Bakan, B; Melcion, D; Richard-Molard, D; Cahagnier, B

    2002-02-13

    Fungi of the genus Fusarium are common fungal contaminants of maize and are also known to produce mycotoxins. Maize that has been genetically modified to express a Bt endotoxin has been used to study the effect of insect resistance on fungal infection of maize grains by Fusarium species and their related mycotoxins. Maize grain from Bt hybrids and near-isogenic traditional hybrids was collected in France and Spain from the 1999 crop, which was grown under natural conditions. According to the ergosterol level, the fungal biomass formed on Bt maize grain was 4-18 times lower than that on isogenic maize. Fumonisin B(1) grain concentrations ranged from 0.05 to 0.3 ppm for Bt maize and from 0.4 to 9 ppm for isogenic maize. Moderate to low concentrations of trichothecenes and zearalenone were measured on transgenic as well as on non-transgenic maize. Nevertheless, significant differences were obtained in certain regions. The protection of maize plants against insect damage (European corn borer and pink stem borer) through the use of Bt technology seems to be a way to reduce the contamination of maize by Fusarium species and the resultant fumonisins in maize grain grown in France and Spain.

  4. Vip3Aa and Cry1Ab proteins in maize reduce Fusarium ear rot and fumonisins by deterring kernel injury from multiple Lepidopteran pests

    Science.gov (United States)

    Field trials were conducted in 2008, 2009 and 2011 to assess fumonisin contamination in transgenic Bacillus thuringiensis (Bt) and non-Bt maize hybrids infested with European corn borer, corn earworm, and Western bean cutworm. Comparisons were made among maize hybrids expressing two transgenic insec...

  5. Utilizing Protein-lean Co-products from Corn Containing Recombinant Pharmaceutical Proteins for Ethanol Production

    Science.gov (United States)

    Protein-lean fractions of corn (maize) containing recombinant (r) pharmaceutical proteins were used to produce fuel ethanol and residual r-proteins in the co-product, distillers dry grains with solubles (DDGS), were determined. Transgenic corn lines containing recombinant green fluorescence protein ...

  6. Comparison of nine PCR primer sets designed to detect Pantoea stewartii subsp. stewartii in maize

    Science.gov (United States)

    Pantoea stewartii subsp. stewartii, the causal agent of Stewart's bacterial wilt of maize, is a major quarantine pest in maize seed. Verifying freedom from P. stewartii remains a significant hurdle in exporting corn seed from the U.S. Several PCR primer sets have been developed and suggested as bein...

  7. Green ear yield and grain yield of maize after harvest of the first ear as baby corn Rendimentos de espigas verdes e de grãos de milho após a colheita da primeira espiga como minimilho

    Directory of Open Access Journals (Sweden)

    Paulo Sérgio L e Silva

    2006-06-01

    Full Text Available Baby corn (BC consists of the corn ear harvested two or three days after silk emergence. BC is a profitable crop, making possible a diversification of production, aggregation of value and increased income. Removing the first female inflorescence induces corn to produce others, making possible to produce several BC ears or, alternatively, BC (by harvesting the first ear and green ears or grain. The objective of this work was to evaluate green ear yield and grain yield, after harvesting the first ear as BC. Corn cultivar AG 1051 was submitted to the following treatments, in a random block design with ten replicates (52 plants per plot: BC harvesting; green ear harvesting (grain moisture content between 60 and 70%; mature ear harvesting; BC harvesting and harvesting of other ears as green or mature ears. Marketable green ears yield or grain yield produced without removing the first inflorescence were superior to the green ears yield or grain yield produced after removal of the first inflorescence harvested as baby corn. Harvesting only the first ear as baby corn, and then harvesting green ears or the mature ears, provided lower baby corn yields than that obtained by harvesting all ears as baby corn. Economically, the best net revenues would be obtained by exploring the crop for the production of green ears, green ears + baby corn, baby corn, baby corn + grain, and grain, in this order.O minimilho (MM é a espiga do milho colhida dois a três dias após a emergência dos estilo-estigmas. O MM é rentável e propicia diversificação da produção, agregação de valor e ampliação de renda. A remoção da primeira inflorescência feminina induz o milho a produzir outras. Isso possibilita a produção de várias espigas de MM ou, alternativamente, MM (colhendo-se a primeira espiga e espigas verdes ou grãos. O objetivo do trabalho foi avaliar os rendimentos de espigas verdes e de grãos, após a colheita da primeira espiga como MM. A cultivar AG

  8. Immunofluorescence localization and ultrastructure of Stewart’s wilt disease bacterium Pantoea stewartii in maize leaves and in its flea beetle vector Chaetocnema pulicaria (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Pantoea stewartii is the causal agent of Stewart's wilt of sweet corn, the most serious bacterial disease of sweet corn and maize in the North-Central and Eastern USA. P. stewartii is transmitted mainly by the corn flea beetle Chaetocnema pulicaria (Coleoptera: Chrysomelidae) and this bacterium is a...

  9. Using Maize chlorotic dwarf virus to explore future frontiers in plant virology

    Science.gov (United States)

    Maize chlorotic dwarf virus (MCDV) causes a chlorosis and stunting disease of corn throughout the Midwest United States. It is a waikavirus transmitted by the leafhopper Graminella nigrifrons. Although waikaviruses are economically important viruses in corn and rice, little is known about the viru...

  10. Sustainable Dry Land Management Model on Corn Agribusiness System

    Directory of Open Access Journals (Sweden)

    Yulia Pujiharti

    2008-01-01

    Full Text Available The study aimed at building model of dry land management. Dynamic System Analysis was used to build model and Powersim 2.51 version for simulating. The parameter used in model were fertilizer (urea, SP-36, ACL, productivity (corn, cassava, mungbean, soil nutrient (N, P, K, crop nutrient requirements (corn, cassava, mungbean, mucuna, price (corn, cassava, mungbeans corn flour, feed, urea, SP-36, KCl, food security credit, area planted of (maize, cassava, mungbean, area harvested of (maize, cassava, mungbean, (corn, cassava, mungbean production, wages and farmer income. Sustainable indicator for ecology aspect was soil fertility level, economic aspects were productivity and farmer income, and social aspects were job possibility and traditions. The simulation result indicated that sustainable dry land management can improve soil fertility and increase farmer revenue, became sustainable farming system and farmer society. On the other hand, conventional dry land management decreased soil fertility and yield, caused farmer earnings to decrease and a farm activity could not be continued. Fertilizer distribution did not fulfill farmer requirement, which caused fertilizer scarcity. Food security credit increased fertilizer application. Corn was processed to corn flour or feed to give value added.

  11. Broad-spectrum resistance to Bacillus thuringiensis toxins by western corn rootworm (Diabrotica virgifera virgifera).

    Science.gov (United States)

    Jakka, Siva R K; Shrestha, Ram B; Gassmann, Aaron J

    2016-06-14

    The evolution of resistance and cross-resistance threaten the sustainability of genetically engineered crops that produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt). Western corn rootworm, Diabrotica virgifera virgifera LeConte, is a serious pest of maize and has been managed with Bt maize since 2003. We conducted laboratory bioassays with maize hybrids producing Bt toxins Cry3Bb1, mCry3A, eCry3.1Ab, and Cry34/35Ab1, which represent all commercialized Bt toxins for management of western corn rootworm. We tested populations from fields where severe injury to Cry3Bb1 maize was observed, and populations that had never been exposed to Bt maize. Consistent with past studies, bioassays indicated that field populations were resistant to Cry3Bb1 maize and mCry3A maize, and that cross-resistance was present between these two types of Bt maize. Additionally, bioassays revealed resistance to eCry3.1Ab maize and cross-resistance among Cry3Bb1, mCry3A and eCry3.1Ab. However, no resistance or cross-resistance was detected for Cry34/35Ab1 maize. This broad-spectrum resistance illustrates the potential for insect pests to develop resistance rapidly to multiple Bt toxins when structural similarities are present among toxins, and raises concerns about the long-term durability of Bt crops for management of some insect pests.

  12. Resistance Evaluation of Btcry1Ah-transgenic Maize to Asian Corn Borer,Cotton Bollworm and Oriental Armyworm%转Bt cry1Ah基因抗虫玉米对亚洲玉米螟、棉铃虫和黏虫的抗性评价

    Institute of Scientific and Technical Information of China (English)

    宋苗; 汪海; 张杰; 何康来; 梁革梅; 朱莉; 黄大昉; 郎志宏

    2016-01-01

    Bt cry1Ah gene was transferred into inbred line maize Zong31 via Agrobacterium-mediated method,and transgenic maize HGK60 with significant resistance to corn borer was acquired. In order to investigate its insecticidal activity to Lepidoptera pests,we evaluated the insecticidal effects of HGK60 to Asian corn boner(Ostrinia furnacalis),cotton bollworm(Mythimna separate(Walker))and oriental armyworm(Helicoverpa armigeraHubne)through laboratory and field bioassay. The results of laboratory bioassay indicated that no Asian corn boner feeding on HGK60 leaves survived. HGK60 presented the toxic effect on neonate of cotton bollworm,and different tissues of it had different insecticidal effects. Compared to the non-transgenic maize,the body-weight of armyworm neonate was significantly inhibited after a week of feeding HGK 60 leaves. The results of field bioassay showed that HGK60 had solid insecticidal effects to O. furnacalis and H. armigera in high resistance level,while the efficacy to M. separate was in resistance level.%利用农杆菌介导法将Btcry1Ah基因转入玉米自交系综31,获得对玉米螟有显著抗性的转基因玉米HGK60,为了研究其对鳞翅目害虫的杀虫活性,在室内和田间分别用亚洲玉米螟、棉铃虫和黏虫幼虫对HGK60玉米的杀虫效果进行检测。室内生物活性检测结果表明,取食HGK60玉米心叶的亚洲玉米螟幼虫在3d后死亡率达100%;HGK60玉米对棉铃虫幼虫有毒杀作用,玉米不同部位对棉铃虫幼虫的杀虫效果存在差异;与非转基因玉米对照相比,取食HGK60玉米叶片一周后的黏虫幼虫体重增长明显受到抑制。田间生物活性检测结果表明,HGK60玉米对于亚洲玉米螟和棉铃虫有很强的杀虫活性,达到高抗级别,对于黏虫有一定的杀虫活性,为抗性级别。

  13. MAIZE POPULATIONS

    African Journals Online (AJOL)

    2003-06-17

    Jun 17, 2003 ... ZM607 at two drought stressed and one well-watered environment for yield and secondary traits. ... et à la grande interaction génotype-environnement entre le Mexique ou dépistage a ...... by CIMMYT and the Maize and Wheat .... Thesis. May, L.H. and Milthorpe, F.L. 1962. Drought resistance of crop plants.

  14. Antagonistic and Biocontrol Potential of Trichoderma asperellum ZJSX5003 Against the Maize Stalk Rot Pathogen Fusarium graminearum

    National Research Council Canada - National Science Library

    Li, Yaqian; Sun, Ruiyan; Yu, Jia; Saravanakumar, Kandasamy; Chen, Jie

    2016-01-01

    ...) the causal agent of corn stalk rot of maize were in vitro for their antagonistic properties followed by statistical model of principal compound analysis to identify the beneficial antagonist T. asperellum strain...

  15. Control of Aspergillus flavus in maize with plant essential oils and their components.

    Science.gov (United States)

    Montes-Belmont, R; Carvajal, M

    1998-05-01

    The effects of 11 plant essential oils for maize kernel protection against Aspergillus flavus were studied. Tests were conducted to determine optimal levels of dosages for maize protection, effects of combinations of essential oils, and residual effects and toxicity of essential oils to maize plants. Principal constituents of eight essential oils were tested for ability to protect maize kernels. Essential oils of Cinnamomum zeylanicum (cinnamon), Mentha piperita (peppermint), Ocimum basilicum (basil), Origanum vulgare (origanum), Teloxys ambrosioides (the flavoring herb epazote), Syzygium aromaticum (clove), and Thymus vulgaris (thyme) caused a total inhibition of fungal development on maize kernels. Thymol and o-methoxycinnamaldehyde significantly reduced maize grain contamination. The optimal dosage for protection of maize varied from 3 to 8%. Combinations of C. zeylanicum with the remaining oils gave efficient control. A residual effect of C. zeylanicum was detected after 4 weeks of kernel treatment. No phytotoxic effect on germination and corn growth was detected with any of these oils.

  16. Arsenic accumulation in maize crop (Zea mays): a review.

    Science.gov (United States)

    Rosas-Castor, J M; Guzmán-Mar, J L; Hernández-Ramírez, A; Garza-González, M T; Hinojosa-Reyes, L

    2014-08-01

    Arsenic (As) is a metalloid that may represent a serious environmental threat, due to its wide abundance and the high toxicity particularly of its inorganic forms. The use of arsenic-contaminated groundwater for irrigation purposes in crop fields elevates the arsenic concentration in topsoil and its phytoavailability for crops. The transfer of arsenic through the crops-soil-water system is one of the more important pathways of human exposure. According to the Food and Agriculture Organization of the United Nations, maize (Zea mays L.) is the most cultivated cereal in the world. This cereal constitutes a staple food for humans in the most of the developing countries in Latin America, Africa, and Asia. Thus, this review summarizes the existing literature concerning the conditions involved in agricultural soil that leads to As influx into maize crops and the uptake mechanisms, metabolism and phytotoxicity of As in corn plants. Additionally, the studies of the As accumulation in raw corn grain and corn food are summarized, and the As biotransfer into the human diet is highlighted. Due to high As levels found in editable plant part for livestock and humans, the As uptake by corn crop through water-soil-maize system may represent an important pathway of As exposure in countries with high maize consumption.

  17. Transgenic approaches to western corn rootworm control.

    Science.gov (United States)

    Narva, Kenneth E; Siegfried, Blair D; Storer, Nicholas P

    2013-01-01

    The western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) is a significant corn pest throughout the United States corn belt. Rootworm larvae feed on corn roots causing yield losses and control expenditures that are estimated to exceed US$1 billion annually. Traditional management practices to control rootworms such as chemical insecticides or crop rotation have suffered reduced effectiveness due to the development of physiological and behavioral resistance. Transgenic maize expressing insecticidal proteins are very successful in protecting against rootworm damage and preserving corn yield potential. However, the high rate of grower adoption and early reliance on hybrids expressing a single mode of action and low-dose traits threatens the durability of commercialized transgenic rootworm technology for rootworm control. A summary of current transgenic approaches for rootworm control and the corresponding insect resistance management practices is included. An overview of potential new modes of action based on insecticidal proteins, and especially RNAi targeting mRNA coding for essential insect proteins is provided.

  18. Romanian maize

    DEFF Research Database (Denmark)

    Sauer, Johannes; Balint, Borbala

    This research aims at shedding empirical light on the relative efficiency of small-scale maize producers in Romania. Farmers in transition countries still face heavily distorted price systems resulting from imperfect market conditions and socioeconomic and institutional constraints. To capture su...... concavity on the shadow cost frontier leads to relative differences in the efficiency es-timates of up to 240%.......This research aims at shedding empirical light on the relative efficiency of small-scale maize producers in Romania. Farmers in transition countries still face heavily distorted price systems resulting from imperfect market conditions and socioeconomic and institutional constraints. To capture...... such distortions we formulate a stochastic shadow-cost frontier model to investigate the systematic input-specific allocative inef-ficiency. We further adjust the underlying cost frontier by incorporating shadow price corrections and subsequently reveal evidence on farm specific technical inefficiency. Different...

  19. Susceptibility and aversion of Spodoptera frugiperda to Cry1F Bt maize and considerations for insect resistance management

    Science.gov (United States)

    Bacillus thuringiensis (Bt) maize was developed primarily for North American pests such as European corn borer (Ostrinia nubilalis Hubner). However, most Bt maize products also are cultivated outside of North America, where the primary pests are different and often have lower susceptibility to Bt to...

  20. A rapid and efficient method for assessing pathogenicity of ustilago maydis on maize and teosinte lines.

    Science.gov (United States)

    Chavan, Suchitra; Smith, Shavannor M

    2014-01-03

    Maize is a major cereal crop worldwide. However, susceptibility to biotrophic pathogens is the primary constraint to increasing productivity. U. maydis is a biotrophic fungal pathogen and the causal agent of corn smut on maize. This disease is responsible for significant yield losses of approximately $1.0 billion annually in the U.S.(1) Several methods including crop rotation, fungicide application and seed treatments are currently used to control corn smut(2). However, host resistance is the only practical method for managing corn smut. Identification of crop plants including maize, wheat, and rice that are resistant to various biotrophic pathogens has significantly decreased yield losses annually(3-5). Therefore, the use of a pathogen inoculation method that efficiently and reproducibly delivers the pathogen in between the plant leaves, would facilitate the rapid identification of maize lines that are resistant to U. maydis. As, a first step toward indentifying maize lines that are resistant to U. maydis, a needle injection inoculation method and a resistance reaction screening method was utilized to inoculate maize, teosinte, and maize x teosinte introgression lines with a U. maydis strain and to select resistant plants. Maize, teosinte and maize x teosinte introgression lines, consisting of about 700 plants, were planted, inoculated with a strain of U. maydis, and screened for resistance. The inoculation and screening methods successfully identified three teosinte lines resistant to U. maydis. Here a detailed needle injection inoculation and resistance reaction screening protocol for maize, teosinte, and maize x teosinte introgression lines is presented. This study demonstrates that needle injection inoculation is an invaluable tool in agriculture that can efficiently deliver U. maydis in between the plant leaves and has provided plant lines that are resistant to U. maydis that can now be combined and tested in breeding programs for improved disease resistance.

  1. Compositional equivalency of Cry1F corn event TC6275 and conventional corn (Zea mays L.).

    Science.gov (United States)

    Herman, Rod A; Phillips, Amy M; Collins, Randy A; Tagliani, Laura A; Claussen, Fred A; Graham, Christopher D; Bickers, Brenda L; Harris, Travis A; Prochaska, Lee M

    2004-05-01

    Maize (Zea mays L.) plants have been transformed to express a Cry1F insecticidal crystal protein originally isolated from Bacillus thuringiensis Berliner. This protein controls lepidopteran pests of maize, including the European corn borer, Ostrinia nubilalis (Hübner). As part of the safety assessment for crops containing transgenes, a compositional analysis of the food and feed is conducted. This analysis is designed to detect unintended changes in the nutrient and antinutrient content of the raw commodities produced by the crop due to the insertion of the genes into the genomic DNA of the plant (pleotropic effects). Samples of transgenic and nontransgenic maize forage and grain were collected from six field sites located in the U.S. and Canada. Forage samples were analyzed for proximates and minerals, and grain was further analyzed for fatty acids, amino acids, vitamins, secondary metabolites, and antinutrients. Results demonstrated that maize expressing the Cry1F protein was equivalent to nontransgenic maize with respect to these important components. Comparison of the variability within the nontransgenic and transgenic hybrid, as compared to composition values reported in the literature, suggest that factors other than transgenes may contribute more substantially to the composition of crops.

  2. Refuge or reservoir? The potential impacts of the biofuel crop Miscanthus x giganteus on a major pest of maize.

    Directory of Open Access Journals (Sweden)

    Joseph L Spencer

    Full Text Available BACKGROUND: Interest in the cultivation of biomass crops like the C4 grass Miscanthus x giganteus (Miscanthus is increasing as global demand for biofuel grows. In the US, Miscanthus is promoted as a crop well-suited to the Corn Belt where it could be cultivated on marginal land interposed with maize and soybean. Interactions (direct and indirect of Miscanthus, maize, and the major Corn Belt pest of maize, the western corn rootworm, (Diabrotica virgifera virgifera LeConte, WCR are unknown. Adding a perennial grass/biomass crop to this system is concerning since WCR is adapted to the continuous availability of its grass host, maize (Zea mays. METHODOLOGY/PRINCIPAL FINDINGS: In a greenhouse and field study, we investigated WCR development and oviposition on Miscanthus. The suitability of Miscanthus for WCR development varied across different WCR populations. Data trends indicate that WCR populations that express behavioural resistance to crop rotation performed as well on Miscanthus as on maize. Over the entire study, total adult WCR emergence from Miscanthus (212 WCR was 29.6% of that from maize (717 WCR. Adult dry weight was 75-80% that of WCR from maize; female emergence patterns on Miscanthus were similar to females developing on maize. There was no difference in the mean no. of WCR eggs laid at the base of Miscanthus and maize in the field. CONCLUSIONS/SIGNIFICANCE: Field oviposition and significant WCR emergence from Miscanthus raises many questions about the nature of likely interactions between Miscanthus, maize and WCR and the potential for Miscanthus to act as a refuge or reservoir for Corn Belt WCR. Responsible consideration of the benefits and risks associated with Corn Belt Miscanthus are critical to protecting an agroecosystem that we depend on for food, feed, and increasingly, fuel. Implications for European agroecosystems in which Miscanthus is being proposed are also discussed in light of the WCR's recent invasion into Europe.

  3. Bacterial endophytes from wild maize suppress Fusarium graminearum in modern maize and inhibit mycotoxin accumulation

    Directory of Open Access Journals (Sweden)

    Walaa Kamel Mousa

    2015-10-01

    Full Text Available Wild maize (teosinte has been reported to be less susceptible to pests than their modern maize (corn relatives. Endophytes, defined as microbes that inhabit plants without causing disease, are known for their ability to antagonize plant pests and pathogens. We hypothesized that the wild relatives of modern maize may host endophytes that combat pathogens. Fusarium graminearum is the fungus that causes Gibberella Ear Rot (GER in modern maize and produces the mycotoxin, deoxynivalenol (DON. In this study, 215 bacterial endophytes, previously isolated from diverse maize genotypes including wild teosintes, traditional landraces and modern varieties, were tested for their ability to antagonize F. graminearum in vitro. Candidate endophytes were then tested for their ability to suppress GER in modern maize in independent greenhouse trials. The results revealed that three candidate endophytes derived from wild teosintes were most potent in suppressing F. graminearum in vitro and GER in a modern maize hybrid. These wild teosinte endophytes could suppress a broad spectrum of fungal pathogens of modern crops in vitro. The teosinte endophytes also suppressed DON mycotoxin during storage to below acceptable safety threshold levels. A fourth, less robust anti-fungal strain was isolated from a modern maize hybrid. Three of the anti-fungal endophytes were predicted to be Paenibacillus polymyxa, along with one strain of Citrobacter. Microscopy studies suggested a fungicidal mode of action by all four strains. Molecular and biochemical studies showed that the P. polymyxa strains produced the previously characterized anti-Fusarium compound, fusaricidin. Our results suggest that the wild relatives of modern crops may serve as a valuable reservoir for endophytes in the ongoing fight against serious threats to modern agriculture. We discuss the possible impact of crop evolution and domestication on endophytes in the context of plant defense.

  4. Impacts of climate change on corn yield and the length of corn growing season in U.S. Corn Belt

    Science.gov (United States)

    Niyogi, D.; Liu, X.; Takle, E. S.; Anderson, C.; Andresen, J.; Alagarswamy, G.; Gramig, B. M.; Doering, O.

    2015-12-01

    This study is a result of a USDA sponsored project titled Useful to Usable (U2U): "Transforming Climate Variability and Change Information for Cereal Crop Producers". The objective of this project is to improve farm resilience and profitability in the U.S. Corn Belt region by transforming existing meteorological dataset into usable knowledge and tools for the agricultural community. In this study, we conducted the Hybrid-Maize corn growth simulation model at 18 sites across the U.S. Corn Belt with 5 CMIP5 (Coupled Model Intercomparison Project) climate models. The crop model was running for two time periods: 1981-2010 ('current') and 2041-2070 ('future'). We also developed a "delta" method, which combines the current climate variability with the "mean" model projected climate change. The results indicate that under the 'future' climate, growing degree days (GDD) projected corn growing season (from planting date reach to maturity required GDD) are shortened due to the increasing of mean temperature. Compare to the contemporary simulations, the shorter growing season under "future" scenario brings lower attainable yields if farmers using the same cultivar. This presentation will focus on the details about the model simulations, the interactive process employed in developing the simulations, the implications of the results, the uncertainties, and the lessons learned.

  5. Preliminary Trial of 11 New Hybrid Maize Genotype to The Resistance on Java Downy Mildew (Peronosclerospora maydis)

    OpenAIRE

    Budi Setyawan; Irfan Suliansyah; Aswaldi Anwar; Etti Swasti

    2016-01-01

    Maize or corn (Zea mays L.) belongs to the family of grasses (Poaceae).  Maize is grown globally and one of the most important cereal crop in the world.  In many countries, corn is the main agricultural crop, and are used as food, feed and industrial raw materials. Together with rice and wheat, corn included in the cereals that provide about 65% carbohydrates and 50% protein that humans need. For this purpose, many developing countries, especially in Asia and Africa are in a strong effort to ...

  6. Delaying corn rootworm resistance to Bt corn.

    Science.gov (United States)

    Tabashnik, Bruce E; Gould, Fred

    2012-06-01

    Transgenic crops producing Bacillus thuringiensis (Bt) toxins for insect control have been successful, but their efficacy is reduced when pests evolve resistance. To delay pest resistance to Bt crops, the U.S. Environmental Protection Agency (EPA) has required refuges of host plants that do not produce Bt toxins to promote survival of susceptible pests. Such refuges are expected to be most effective if the Bt plants deliver a dose of toxin high enough to kill nearly all hybrid progeny produced by matings between resistant and susceptible pests. In 2003, the EPA first registered corn, Zea mays L., producing a Bt toxin (Cry3Bb1) that kills western corn rootworm, Diabrotica virgifera virgifera LeConte, one of the most economically important crop pests in the United States. The EPA requires minimum refuges of 20% for Cry3Bb1 corn and 5% for corn producing two Bt toxins active against corn rootworms. We conclude that the current refuge requirements are not adequate, because Bt corn hybrids active against corn rootworms do not meet the high-dose standard, and western corn rootworm has rapidly evolved resistance to Cry3Bb1 corn in the laboratory, greenhouse, and field. Accordingly, we recommend increasing the minimum refuge for Bt corn targeting corn rootworms to 50% for plants producing one toxin active against these pests and to 20% for plants producing two toxins active against these pests. Increasing the minimum refuge percentage can help to delay pest resistance, encourage integrated pest management, and promote more sustainable crop protection.

  7. Significant yield increases from control of leaf diseases in maize - an overlooked problem?!

    DEFF Research Database (Denmark)

    Jørgensen, Lise Nistrup

    2012-01-01

    The area of maize has increased in several European countries in recent years. In Denmark, the area has increased from 10,000 ha in 1980 to 185,000 ha in 2011. Initially only silage maize was cultivated in Denmark, but in more recent years the area of grain maize has also increased. Farms growing...... maize have often grown maize consecutively for several years. This leads to significant amounts of debris building up in the fields, which serves as an important source of inoculum for diseases when new crops are grown. In recent years, leaf diseases have caused production problems over several seasons....... Two major diseases have been identified: Eyespot (Kabatiella zeae) and Northern leaf blotch (Exserohilum turcicum). Other diseases, including Southern maize leaf blight or maydis leaf blight, caused by Bipolaris maydis, and Northern corn leaf spot, caused by Bipolaris zeicola, may potentially play...

  8. Zapatista corn: a case study in biocultural innovation.

    Science.gov (United States)

    Brandt, Marisa

    2014-12-01

    In November 2001, Nature published a letter in which University of California Berkeley's biologists claimed to have found evidence of genetically modified (GM) DNA in regional varieties of maize in Oaxaca, even though the Mexican government had banned transgenic corn agriculture in 1998. While urban protesters marched against the genetic 'contamination' of Mexican corn by US-based agricultural biotech firms, rural indigenous communities needed a framework for understanding concepts such as GM before they could take action. This article analyzes how the indigenous organization, the Zapatistas, mobilized a program to address this novel entity. Their anti-GM project entailed educating local farmers about genetics, importing genetic testing kits, seed-banking landrace corn and sending seeds to 'solidarity growers' around the world. This article explores material-semiotic translations to explain one of the central aspects of this project, the definition and circulation of Zapatista corn--an entity defined not only through cultural geography, but also technological means. Through its circulation, Zapatista corn serves to perform a biocultural engagement with Zapatista's political project of resistance to neoliberalism. While much has been written about both regulatory policy and consumer activism against GM in the Global North, Zapatista corn also provides a case study in indigenous, anti-GM activism founded on biocultural innovation and the creation of alternative networks for circulating corn.

  9. Physical and Chemical Properties and Safety Study of Six Corn Seed-coatings to Maize%6种玉米种衣剂的理化性质测定及其安全性研究

    Institute of Scientific and Technical Information of China (English)

    王斌; 张婧赢; 王岩; 郑逢云; 刘三明

    2011-01-01

    To better understand the quality of corn seed-coatings, physical and chemical properties and safety of six corn seed-coatings were tested.Film-forming, Coating homogeneity and suspension rate of all coatings met the national standards.Viscosity values of 15.5% Thiram · Carbofuran and 8.1% Carbofuran ·Tebuconazole· Triadimenfon reached the national standards.pH ranges of all seed coatings occurred between 6.38 and 7.55, and 25% Carbofuran· Thiram· Carboxin, 15% Thiram· Carbofuran· Tebuconazole and 20% Thiram· Carbofuran · Triadimenal came up to the National Standards.Pull-out rate of seed coatings met the national standards excerpt 25% Carbofuran· Thiram· Carboxin.Germination potential, germination rate,germination index, date of seedling vigor index, seedling length and fresh weight of seeds treated with seed coating were lower than the control in Laboratory.Seedling emergency rates of all treatments in field trail were improved comparing with the control, but seedling quality had no significant increase.The results showed that 15.5% Thiram· Carbofuran is the best choice among the six corn seed-coatings.%为了解市场上玉米种衣剂的质量,对6种市售玉米种衣剂的理化性质和安全性进行了测定.理化性质表明:6种种衣剂的成膜时间、包衣均匀度和悬浮率都符合国家标准;不同种衣剂粘度值差异显著,只有福·克和克·戊·三唑酮符合国家标准;pH都在6.83~7.55之间,其中克·福·萎、福·克·唑醇和克·醇·福美双符合国家标准;包衣脱落率只有克·福·萎大于10%,其它药剂均达到国家标准.室内发芽实验表明:经玉米种衣剂处理后,玉米种子活力等各项指标均低于对照;而田间实验表明:种衣剂处理后,玉米出苗率、幼苗素质与对照相比都有所提高,其中福·克效果最为显著.从室内、田间的实验结果分析,福·克是6种玉米种衣剂中的最佳选择.

  10. Responses by earthworms to reduced tillage in herbicide tolerant maize and Bt maize cropping systems

    DEFF Research Database (Denmark)

    Krogh, P. H.; Griffiths, B.; Demsar, D.

    2007-01-01

    Genetically modified crops (GM) may affect earthworms either directly through the plant, its root exudates and litter, or indirectly through the agricultural management changes that are associated with GM plant production. In order to investigate such possible effects we established two field...... studies of Bt corn and a glufosinate ammonium tolerant corn and included a reduced tillage treatment (RT) and a conventional tillage treatment (CT) as examples of a likely concomitant change in the agricultural practise. At a French study site at Varois, (Bourgogne), a field grown with the Bt......-toxin producing transgenic maize line MON810 was studied for 1 year. At a Danish study site, Foulum (Jutland), one year of Bt corn was followed by 2 years of herbicide tolerant corn. At the French study site the most prominent effects observed were due to the tillage method where RT significantly reduced...

  11. Research Progress on Northern Leaf Blight in Corn

    Institute of Scientific and Technical Information of China (English)

    ZHAO Yingnan; WANG Zhenhua

    2009-01-01

    The northern corn leaf blight is one of the most serious diseases in maize production. The research progress on etiology, generation law and control strategy, antigen identification, resistance mechanism and inheritance of this disease was discussed. And the research work which should be enhanced in China was pointed out, such as mechanism of resistance inheritance, developing function marker, gene mining, screening resistance resource and dominant physiological race in different areas.

  12. Introducing Bt Gene Into Maize With Ovary Injection

    Institute of Scientific and Technical Information of China (English)

    丁群星; 谢友菊; 戴景瑞; 米景九; 李太元; 田颖川; 乔利亚; 莽克强; 刘宝兰; 王音; 冯平章

    1994-01-01

    It is reported here that Bt toxin gene has been successfully transferred into maize inbred line by ovary injection for the first time both at home and abroad. One transgenic plant (To) has been confirmed by Southern blotting and PCR test, and 71 progenies (T1) from T0 have been obtained through self-pollination. Of these 71 progenies, seven plants demonstrated positive results in the PCR test; four were used to feed Asian corn borer, and certain effect of insect-resistance was observed. The experiments on the ovary injection in Hainan Province have also been repeated, thus providing new chance to the application of genetic engineering to the maize improvement.

  13. THE USE OF ENDOPHYTIC FUNGI AS BIOPESTICIDE AGAINST DOWNY MILDEW PERONOSCLEROSPORA SPP. ON MAIZE

    OpenAIRE

    AMIN, NUR; La Daha; Nasruddin, Andi; Junaed, M; Iqbal, Andi

    2013-01-01

    Downy mildew is a major disease of maize caused by the fungus Peronosclerospora spp., widely distributed in all corn production centers in Indonesia. The disease can cause considerable losses; even total losses have been reported occurring on susceptible varieties. The purpose of the research was to determine the effectiveness of some isolates of endophytic fungi for the control of downy mildew on maize. The study consisted of four isolate treatments: Aspergillus spp., Trichoderma spp, Beauv...

  14. Disruption of ceramide biosynthesis and accumulation of sphingoid bases and sphingoid base 1-phosphates: A mechanism for Fusarium verticillioides effects in maize-seedling disease.

    Science.gov (United States)

    In sweet corn at the seedling and seed maturation stages, Fusarium can be a serious field problem. The fungus Fusarium verticillioides infects maize and produces fumonisins, inhibitors of ceramide synthase. To determine the role of fumonisins in maize seedling disease, seeds were inoculated with fu...

  15. Diversity in global maize germplasm: Characterization and utilization

    Indian Academy of Sciences (India)

    B M Prasanna

    2012-11-01

    Maize (Zea mays L.) is not only of worldwide importance as a food, feed and as a source of diverse industrially important products, but is also a model genetic organism with immense genetic diversity. Although it was first domesticated in Mexico, maize landraces are widely found across the continents. Several studies in Mexico and other countries highlighted the genetic variability in the maize germplasm. Applications of molecular markers, particularly in the last two decades, have led to new insights into the patterns of genetic diversity in maize globally, including landraces as well as wild relatives (especially teosintes) in Latin America, helping in tracking the migration routes of maize from the centers of origin, and understanding the fate of genetic diversity during maize domestication. The genome sequencing of B73 (a highly popular US Corn Belt inbred) and Palomero (a popcorn landrace in Mexico) in the recent years are important landmarks in maize research, with significant implications to our understanding of the maize genome organization and evolution. Next-generation sequencing and high-throughput genotyping platforms promise to further revolutionize our understanding of genetic diversity and for designing strategies to utilize the genomic information for maize improvement. However, the major limiting factor to exploit the genetic diversity in crops like maize is no longer genotyping, but high-throughput and precision phenotyping. There is an urgent need to establish a global phenotyping network for comprehensive and efficient characterization of maize germplasm for an array of target traits, particularly for biotic and abiotic stress tolerance and nutritional quality. ‘Seeds of Discovery’ (SeeD), a novel initiative by CIMMYT with financial support from the Mexican Government for generating international public goods, has initiated intensive exploration of phenotypic and molecular diversity of maize germplasm conserved in the CIMMYT Gene Bank; this

  16. Diversity in global maize germplasm: characterization and utilization.

    Science.gov (United States)

    Prasanna, B M

    2012-11-01

    Maize (Zea mays L.) is not only of worldwide importance as a food, feed and as a source of diverse industrially important products, but is also a model genetic organism with immense genetic diversity. Although it was first domesticated in Mexico, maize landraces are widely found across the continents. Several studies in Mexico and other countries highlighted the genetic variability in the maize germplasm. Applications of molecular markers, particularly in the last two decades, have led to new insights into the patterns of genetic diversity in maize globally, including landraces as well as wild relatives (especially teosintes) in Latin America, helping in tracking the migration routes of maize from the centers of origin, and understanding the fate of genetic diversity during maize domestication. The genome sequencing of B73 (a highly popular US Corn Belt inbred) and Palomero (a popcorn landrace in Mexico) in the recent years are important landmarks in maize research, with significant implications to our understanding of the maize genome organization and evolution. Next-generation sequencing and high-throughput genotyping platforms promise to further revolutionize our understanding of genetic diversity and for designing strategies to utilize the genomic information for maize improvement. However, the major limiting factor to exploit the genetic diversity in crops like maize is no longer genotyping, but high-throughput and precision phenotyping. There is an urgent need to establish a global phenotyping network for comprehensive and efficient characterization of maize germplasm for an array of target traits, particularly for biotic and abiotic stress tolerance and nutritional quality. 'Seeds of Discovery' (SeeD), a novel initiative by CIMMYT with financial support from the Mexican Government for generating international public goods, has initiated intensive exploration of phenotypic and molecular diversity of maize germplasm conserved in the CIMMYT Gene Bank; this is

  17. Dietas contendo silagem de milho (Zea maiz L. e feno de capim-tifton 85 (Cynodon spp. em diferentes proporções para bovinos Corn silage and tifton 85 bermudagrass hay-based diets for steers

    Directory of Open Access Journals (Sweden)

    Ana Clara Rodrigues Cavalcante

    2004-12-01

    Full Text Available Avaliaram-se o consumo, a digestibilidade, o pH e concentração de amônia ruminais e a taxa de passagem em bovinos alimentados com dietas contendo feno de capim-tifton 85 (TIF e silagem de milho (SM em diferentes proporções. Utilizaram-se quatro animais castrados, com peso médio de 523kg, fistulados no rúmen, distribuídos em um quadrado latino 4 x 4, recebendo 60% de volumoso e 40% de concentrado, na base da matéria seca. O volumoso consistiu das seguintes proporções (%: 100 TIF:0 SM; 67 TIF:33 SM; 33 TIF:67 SM e 0 TIF:100 SM. Os consumos de matéria seca (MS, de matéria orgânica (MO e de matéria orgânica digestível (MOD, de proteína bruta (PB, de fibra em detergente neutro (FDN e de carboidratos totais (CHOT e digeríveis (CHOD não foram influenciados pelas diferentes proporções de feno de capim-tifton 85: silagem de milho nas dietas, registrando-se valores médios de 9,2; 8,7; 6,4; 1,1; 3,7; 7,3 e 5,4 kg/dia, respectivamente. O consumo de extrato etéreo (EE elevou linearmente com o aumento da silagem de milho nas dietas. As digestibilidades aparentes da MS, MO, PB, EE e CHO também não foram influenciadas pelas diferentes proporções de silagem de milho, obtendo-se, respectivamente, valores médios de 71,8; 73,0; 69,5; 69,2 e 73,9%. Para a digestibilidade da FDN, observou-se efeito quadrático, estimando-se valor máximo de 65,2% para dietas contendo 30,98% de silagem de milho. Estimou-se concentração máxima de amônia de 12,0 mg/100 ml e valor mínimo de pH de 5,98 às 2,44 e 6,82 horas após a alimentação, respectivamente. A taxa de passagem não foi influenciada pelas diferentes proporções de feno:silagem, no volumoso, apresentando valor médio de 4,2%/hora.The intake, digestibility, ruminal pH and ammonia concentrations and passage rate were evaluated in steers fed corn silage (CS and Tifton 85 bermudagrass hay (T85H-based diets, at different forage proportions. Four rumen fistulated steers with 523 kg of live

  18. Spatial patterns of aflatoxin levels in relation to ear-feeding insect damage in pre-harvest corn.

    Science.gov (United States)

    Ni, Xinzhi; Wilson, Jeffrey P; Buntin, G David; Guo, Baozhu; Krakowsky, Matthew D; Lee, R Dewey; Cottrell, Ted E; Scully, Brian T; Huffaker, Alisa; Schmelz, Eric A

    2011-07-01

    Key impediments to increased corn yield and quality in the southeastern US coastal plain region are damage by ear-feeding insects and aflatoxin contamination caused by infection of Aspergillus flavus. Key ear-feeding insects are corn earworm, Helicoverpa zea, fall armyworm, Spodoptera frugiperda, maize weevil, Sitophilus zeamais, and brown stink bug, Euschistus servus. In 2006 and 2007, aflatoxin contamination and insect damage were sampled before harvest in three 0.4-hectare corn fields using a grid sampling method. The feeding damage by each of ear/kernel-feeding insects (i.e., corn earworm/fall armyworm damage on the silk/cob, and discoloration of corn kernels by stink bugs), and maize weevil population were assessed at each grid point with five ears. The spatial distribution pattern of aflatoxin contamination was also assessed using the corn samples collected at each sampling point. Aflatoxin level was correlated to the number of maize weevils and stink bug-discolored kernels, but not closely correlated to either husk coverage or corn earworm damage. Contour maps of the maize weevil populations, stink bug-damaged kernels, and aflatoxin levels exhibited an aggregated distribution pattern with a strong edge effect on all three parameters. The separation of silk- and cob-feeding insects from kernel-feeding insects, as well as chewing (i.e., the corn earworm and maize weevil) and piercing-sucking insects (i.e., the stink bugs) and their damage in relation to aflatoxin accumulation is economically important. Both theoretic and applied ramifications of this study were discussed by proposing a hypothesis on the underlying mechanisms of the aggregated distribution patterns and strong edge effect of insect damage and aflatoxin contamination, and by discussing possible management tactics for aflatoxin reduction by proper management of kernel-feeding insects. Future directions on basic and applied research related to aflatoxin contamination are also discussed.

  19. Spatial Patterns of Aflatoxin Levels in Relation to Ear-Feeding Insect Damage in Pre-Harvest Corn

    Directory of Open Access Journals (Sweden)

    Alisa Huffaker

    2011-07-01

    Full Text Available Key impediments to increased corn yield and quality in the southeastern US coastal plain region are damage by ear-feeding insects and aflatoxin contamination caused by infection of Aspergillus flavus. Key ear-feeding insects are corn earworm, Helicoverpa zea, fall armyworm, Spodoptera frugiperda, maize weevil, Sitophilus zeamais, and brown stink bug, Euschistus servus. In 2006 and 2007, aflatoxin contamination and insect damage were sampled before harvest in three 0.4-hectare corn fields using a grid sampling method. The feeding damage by each of ear/kernel-feeding insects (i.e., corn earworm/fall armyworm damage on the silk/cob, and discoloration of corn kernels by stink bugs, and maize weevil population were assessed at each grid point with five ears. The spatial distribution pattern of aflatoxin contamination was also assessed using the corn samples collected at each sampling point. Aflatoxin level was correlated to the number of maize weevils and stink bug-discolored kernels, but not closely correlated to either husk coverage or corn earworm damage. Contour maps of the maize weevil populations, stink bug-damaged kernels, and aflatoxin levels exhibited an aggregated distribution pattern with a strong edge effect on all three parameters. The separation of silk- and cob-feeding insects from kernel-feeding insects, as well as chewing (i.e., the corn earworm and maize weevil and piercing-sucking insects (i.e., the stink bugs and their damage in relation to aflatoxin accumulation is economically important. Both theoretic and applied ramifications of this study were discussed by proposing a hypothesis on the underlying mechanisms of the aggregated distribution patterns and strong edge effect of insect damage and aflatoxin contamination, and by discussing possible management tactics for aflatoxin reduction by proper management of kernel-feeding insects. Future directions on basic and applied research related to aflatoxin contamination are also

  20. Evaluation of the European Union Maize Landrace Core Collection for resistance to Sesamia nonagrioides (Lepidoptera: Noctuidae) and Ostrinia nubilalis (Lepidoptera: Crambidae).

    Science.gov (United States)

    Malvar, R A; Butrón, A; Alvarez, A; Ordás, B; Soengas, P; Revilla, L P; Ordás, A

    2004-04-01

    Two corn borer species are the principal maize insect pests in Europe, the European corn borer, Ostrinia nubilalis (Hübner), and the pink stem borer, Sesamia nonagrioides (Lefebvre). Hence, it would be advisable to evaluate the European maize germplasm for corn borer resistance to generate European varieties resistant to corn borer attack. The creation of the European Union Maize Landrace Core Collection (EUMLCC) allowed the screening of most of the variability for European corn borer resistance present among European maize local populations from France, Germany, Greece, Italy, Portugal, and Spain, testing a representative sample. The objective of this study was the evaluation of stem and ear resistance of the EUMLCC to European corn borer and pink stem borer attack. Trials were made at two Spanish locations that represent two very different maize-growing areas. Populations that performed relatively well under corn borer infestation for stem and ear damage were 'PRT0010008' and'GRC0010085', among very early landraces; 'PRT00100120' and 'PRT00100186', among early landraces; 'GRC0010174', among midseason landraces; and 'ESP0070441', among late landraces. Either the selection that could have happen under high insect pressure or the singular origin of determined maize populations would be possible explanations for the higher corn borer resistance of some landraces. Landraces 'PRT0010008', 'FRA0410090', 'PRT00100186', and 'ESP0090214' would be selected to constitute a composite population resistant to corn borers and adapted to short season, whereas populations 'ESP0090033', 'PRT00100530', 'GRC0010174', and 'ITA0370005' would be used to make a resistant composite adapted to longer season.

  1. Huitlacoche yield in some maize varieties in the Mediterranean region of Turkey

    Directory of Open Access Journals (Sweden)

    Mehmet Aydoğdu

    2015-06-01

    Full Text Available Huitlacoche is the Aztecs name given to the smut galls on ears of maize caused by the pathogenic plant fungus Ustilago maydis [(DC Corda.]. It is known as maize mushroom, and it has been considered a delicacy and in Mesoamerica. The aim of the present study was to determine the responses of some maize varieties to the growth of the fungus in order to evaluate the prospect production of these smutty ears as a maize mushroom. A 2-year study was conducted in the Mediterranean region of Turkey in 2010 and 2011. Inoculations were performed by injecting inoculum into the ear through the silk channel of plants in plots. Each treatment had control plots. Average ear-gall (huitlacoche severity and incidence of all the varieties were at the rates of 4.0 and 41.6%, respectively. However, the highest severity of ear-gall (6.5 and incidence (60.6% were found in Karadeniz Yıldızı flint maize variety; colossal smutty ears were formed in the maize cultivars. This study showed that certain maize cultivars (flint corn and dent corn can be used efficiently in the production of huitlacoche.

  2. Bioavailability of iron, zinc, folic acid, and vitamin A from fortified maize.

    Science.gov (United States)

    Moretti, Diego; Biebinger, Ralf; Bruins, Maaike J; Hoeft, Birgit; Kraemer, Klaus

    2014-04-01

    Several strategies appear suitable to improve iron and zinc bioavailability from fortified maize, and fortification per se will increase the intake of bioavailable iron and zinc. Corn masa flour or whole maize should be fortified with sodium iron ethylenediaminetetraacetate (NaFeEDTA), ferrous fumarate, or ferrous sulfate, and degermed corn flour should be fortified with ferrous sulfate or ferrous fumarate. The choice of zinc fortificant appears to have a limited impact on zinc bioavailability. Phytic acid is a major inhibitor of both iron and zinc absorption. Degermination at the mill will reduce phytic acid content, and degermed maize appears to be a suitable vehicle for iron and zinc fortification. Enzymatic phytate degradation may be a suitable home-based technique to enhance the bioavailability of iron and zinc from fortified maize. Bioavailability experiments with low phytic acid-containing maize varieties have suggested an improved zinc bioavailability compared to wild-type counterparts. The bioavailability of folic acid from maize porridge was reported to be slightly higher than from baked wheat bread. The bioavailability of vitamin A provided as encapsulated retinyl esters is generally high and is typically not strongly influenced by the food matrix, but has not been fully investigated in maize.

  3. Research Progress of Corn Gluten Meal%玉米蛋白粉研究进展

    Institute of Scientific and Technical Information of China (English)

    潘旭琳; 曹龙奎

    2013-01-01

    玉米蛋白粉是玉米淀粉湿法生产的副产物。文中介绍了玉米蛋白粉的组成及营养价值,综述了玉米蛋白粉深加工的新方法和新工艺,包括玉米醇溶蛋白和玉米黄色素的提取、玉米抗氧化肽的制备以及它们的应用。%Corn gluten meal was the by-product of producing the starch in corn wet-milling process. This paper introduced the composition and nutrition of corn gluten meal,and summarized the new method and technology of corn gluten meal deep processing, which included the technology of extracting zein and corn yellow pigment and preparing antioxidant maize peptides and their application.

  4. Sistemas de control de malezas en maiz (Zea mays L.: efecto de metodos de control, densidad y distribucion del cultivo Weed control systems in corn: effects of control methods, density and plant distribution

    Directory of Open Access Journals (Sweden)

    G. Martinez

    1982-12-01

    realización de una escarda adicional no mejora significativamente el control de malezas, no afectando tampoco la incidencia de enfermedades, ni el desarrollo del cultivo, por lo cual resulta innecesaria. Las escardas tienen como principal efecto eliminar la interfe rencia presentada por las malezas y si éstas son eliminadas de otra manera, la realización de aquéllas no apareja beneficios significativos.An experiment was conducted at the Research Station of the University of Chapingo (Mexico (2250 m above sea level, average annual rainfall 550 mm( loamy soil, 1,7% O.M., where different cultural practices were combined in order to design a weed control system for corn. The work was done under rainfed conditions and the variables included were: two population densities (44.400 and 66.600 pl/ha, two plant distributions (normal and equidistant and seven weed control methods (cyanazine + alachlor (1,2 + 1,92 kg/ha, atrazine + alachlor (1,2 + 1,44 kg/ha, one cultivation, two cultivations, a weeded check, a weeded check + two cultivations and a weedy check. The main weed species were: pigweed (Amaranthus sp., Lopezia mexicana Jacq., hairy galinsoga (Galin-soga parviflora Cay., Encelia mexicana Mart., Sporobulus poiretti (Roem. et Sch. Hich., and large crabgrass (Digitaria sanguinalis (L. Scop.. The increase in plant population had no effect on the degree of weed control, incidence of diseases and crop growth. The equidistant distribution improved weed control over the normal one, but it also had a nigher incidence of diseases; this, is turn, may have caused the lack of differences in vegetative growth, a lower amount of cobs per ha and the lack of difference in grain yield between the two distributions. Atrazine + alachlor was better than cyanazine + alachlor in terms of weed control, although the difference was statistically observed only for the visual ratings. There were no differences between both chemical treatments in terms of incidence of diseases or their effect on crop

  5. Actinomycetales from corn.

    Science.gov (United States)

    Lyons, A J; Pridham, T G; Rogers, R F

    1975-02-01

    Mesophilic Actinomycetales were isolated from whole corn, brewers grits, and break flour received from three different mills. In addition, strains were isolated from high-moisture (27 per cent) field corn; high-moisture, silo-stored corn (untreated); and high-moisture corn treated with ammonia, ammonium isobutyrate, or propionic-acetic acid. According to standard techniques, 139 strains were extensively characterized and 207 additional strains were partially characterized. On the basis of these characterizations, the streptomycete strains were identified by both the systems of Pridham et al. and Hütter because these systems are rapid and accurate. In general, only Streptomyces griseus (Krainsky) Waksman and Henrici was isolated from high-moisture whole corn (treated or untreated) except from grain exposed to ammonium isobutyrate. Strains isolated from high-moisture corn subjected to that treatment represented both S. griseus and S. albus (Rossi Doria) Waksman and Henrici. The strains isolated from corn and corn products from the three mills were identified with a number of streptomycete species. Of all Actinomycetales isolated, only three were not streptomycetes--two from brewer's grits and one from break flour.

  6. Growth and yield of maize in purple nutsedge interference

    Directory of Open Access Journals (Sweden)

    Daniel Valadão Silva

    2015-10-01

    Full Text Available This research aimed evaluates the influence of purple nutsedge (Cyperus rotundus on the agronomic performance of maize. We conducted a field experiment at Viçosa, MG, in completely randomized design experimental with three replications. The treatments consisted of the interaction of maize with nine nutsedge densities (0, 10, 30, 50, 90, 110, 130, 160 and 260 plants m-2. In the flowering culture was made the measurement of plant height, the insertion of the corn ears and the total dry matter of corn plants. At harvest were determined ear weight, grain yield and 100-grain weight adjusted to 13% moisture. Higher density of nutsedge increased plant height and the insertion of the first corn ear. We also observed a reduction in total dry matter accumulation, ear weight, 100-grain weight and grain yield in all densities evaluate. Nutsedge control, even at low densities is necessary because the presence of this plant promotes negative effect on growth and development of corn with a direct consequences on crop productivity.

  7. Effects of Homofermentative Lactic Acid Bacterial Inoculants on the Fermentation and Aerobic Stability Characteristics of Low Dry Matter Corn Silages

    OpenAIRE

    SUCU, Ekin; FİLYA, İsmail

    2014-01-01

    This study was carried out to determine the effects of homofermentative lactic acid bacterial inoculants on the fermentation and aerobic stability characteristics of low dry matter corn silages. Corn was harvested at the milk stage. Inoculant-1188 (Pioneer®, USA; Inoculant A) and Maize-All (Alltech, UK; Inoculant B) were used as homofermentative lactic acid bacterial inoculants. Inoculants were applied to silages 1.5 x 106 colony forming units/g levels. Silages with no additive served as co...

  8. Maize Genetic Resources

    Science.gov (United States)

    This chapter describes the resources held at the Maize Genetics Cooperation • Stock Center in detail and also provides some information about the North Central Regional Plant Introduction Station (NCRPIS) in Ames, IA, Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT) in Mexico, and the N...

  9. Blisters, Calluses, and Corns

    Science.gov (United States)

    ... Too Tall or Too Short All About Puberty Blisters, Calluses, and Corns KidsHealth > For Kids > Blisters, Calluses, ... the surfaces is your tender skin! What's a Blister? A blister is an area of raised skin ...

  10. Corns and calluses

    Science.gov (United States)

    ... rowers get calluses on their hands that prevent blisters from forming. People with bunions often develop a callus over the bunion because it rubs against the shoe. Corns and calluses are not serious problems. Symptoms ...

  11. Intraguild Competition of Three Noctuid Maize Pests.

    Science.gov (United States)

    Bentivenha, J P F; Baldin, E L L; Hunt, T E; Paula-Moraes, S V; Blankenship, E E

    2016-08-01

    The western bean cutworm Striacosta albicosta (Smith), the fall armyworm Spodoptera frugiperda (J. E. Smith), and the corn earworm Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) are among the major lepidopteran pests of maize in the United States, belonging to the same guild and injuring the reproductive tissues of this crop. Here, intraguild competition of these lepidopterans on non-Bt maize was evaluated through survival analysis of each species under laboratory and field conditions. Competition scenarios were carried out in arenas containing maize silk or ear tissue, using larvae on different stadium of development. Fitness cost competition studies were conducted to examine the influence of intraguild competition and cannibalism and predation rates on larval development. The survival of S. albicosta competing with the other species was significantly lower than in intraspecific competition, even when the larvae were more developed than the competitor. For S. frugiperda, survival remained high in the different competition scenarios, except when competing in a smaller stadium with H. zea Larvae of H. zea had a high rate of cannibalism, higher survival when competing against S. albicosta than S. frugiperda, and reduced survival when the H. zea larvae were at the same development stadium or smaller than the competitors. Based on fitness cost results, the absence of a competitor for the feeding source may confer an advantage to the larval development of S. frugiperda and H. zea Our data suggest that S. frugiperda has a competitive advantage against the other species, while S. albicosta has the disadvantage in the intraguild competition on non-Bt maize.

  12. Assessment of the Nutritive Value of Whole Corn Stover and Its Morphological Fractions

    Directory of Open Access Journals (Sweden)

    H. Y. Li

    2014-02-01

    Full Text Available This study investigated the chemical composition and ruminal degradability of corn stover in three maize-planting regions in Qiqihaer, Heilongjiang Province, China. The whole stover was separated into seven morphological fractions, i.e., leaf blade, leaf sheath, stem rind, stem pith, stem node, ear husk, and corn tassel. The assessment of nutritive value of corn stover and its fractions was performed based on laboratory assays of the morphological proportions, chemical composition, and in situ degradability of dry matter (DM, neutral detergent fiber (NDF, and acid detergent fiber (ADF. The chemical composition of corn stover was significantly different from plant top to bottom (p<0.05. Among the whole corn stover and seven morphological fractions, leaf blade had the highest crude protein (CP content and the lowest NDF and ADF contents (p<0.05, whereas stem rind had the lowest CP content and the highest ADF and acid detergent lignin (ADL contents (p<0.05. Ear husk had significantly higher NDF content and relatively lower ADL content than other corn stover fractions. Overall, the effective degradability of DM, NDF, and ADF in rumen was the highest in leaf blade and stem pith, followed by ear husk. The results indicate that leaf blade, ear husk, and stem pith potentially have higher nutritive values than the other fractions of corn stover. This study provides reference data for high-efficiency use of corn stover in feeding ruminants.

  13. Transfer of Bt-toxin protein gene into maize by high-velocity microprojectile bombardments and regeneration of transgenic plants

    Institute of Scientific and Technical Information of China (English)

    王国英; 杜天兵; 张宏; 谢友菊; 戴景瑞; 米景九; 李太源; 田颖川; 乔利亚; 莽克强

    1995-01-01

    Bt-toxin protein gene was successfully transferred into maize by the microprojectile bombard-ments of cell suspension,embryogenic calli and immature embryos with a Chinese-made particle gun(JQ-700).Although the bombarded embryogenic calli and immature embryos produced less mean transformants per dishthan the cell suspensions,they were the suitable materials for maize transformation because their culture andregeneration have been achieved in most maize cultivars.The evaluation on the resistance of transgenic plantsto corn borer shows the significant difference between them,from highly resistant to susceptible.

  14. Framework to Delay Corn Rootworm Resistance

    Science.gov (United States)

    This proposed framework is intended to delay the corn rootworm pest becoming resistant to corn genetically engineered to produce Bt proteins, which kill corn rootworms but do not affect people or wildlife. It includes requirements on Bt corn manufacturers.

  15. Intraplant communication in maize contributes to defense against insects.

    Science.gov (United States)

    Varsani, Suresh; Basu, Saumik; Williams, W Paul; Felton, Gary W; Luthe, Dawn S; Louis, Joe

    2016-08-01

    The vasculature of plants act as a channel for transport of signal(s) that facilitate long-distance intraplant communication. In maize, Maize insect resistance1-Cysteine Protease (Mir1-CP), which has homology to papain-like proteases, provides defense to different feeding guilds of insect pests. Furthermore, accumulation of Mir1-CP in the vasculature suggests that Mir1-CP can potentially function as a phloem-mobile protein. In a recent study, we provided evidence that Mir1-CP can curtail the growth of phloem-sap sucking insect, corn leaf aphid (CLA; Rhopalosiphum maidis). Our current study further examined whether aboveground feeding by CLA can induce resistance to subsequent herbivory by belowground feeding western corn rootworm (WCR; Diabrotica virgifera virgifera). Aboveground feeding by CLA systemically induced the accumulation of Mir1-CP in the roots. Furthermore, foliage feeding by CLA provided enhanced resistance to subsequent herbivory by belowground feeding of WCR. Taken together, our previous findings and results presented here indicate that long-distance transport of Mir1-CP is critical for providing enhanced resistance to insect attack in maize.

  16. Corn Storage Protein - A Molecular Genetic Model

    Energy Technology Data Exchange (ETDEWEB)

    Messing, Joachim [Rutgers, The State University of New Jersey

    2013-05-31

    Corn is the highest yielding crop on earth and probably the most valuable agricultural product of the United States. Because it converts sun energy through photosynthesis into starch and proteins, we addressed energy savings by focusing on protein quality. People and animals require essential amino acids derived from the digestion of proteins. If proteins are relatively low in certain essential amino acids, the crop becomes nutritionally defective and has to be supplemented. Such deficiency affects meat and fish production and countries where corn is a staple. Because corn seed proteins have relatively low levels of lysine and methionine, a diet has to be supplemented with soybeans for the missing lysine and with chemically synthesized methionine. We therefore have studied genes expressed during maize seed development and their chromosomal organization. A critical technical requirement for the understanding of the molecular structure of genes and their positional information was DNA sequencing. Because of the length of sequences, DNA sequencing methods themselves were insufficient for this type of analysis. We therefore developed the so-called “DNA shotgun sequencing” strategy, where overlapping DNA fragments were sequenced in parallel and used to reconstruct large DNA molecules via overlaps. Our publications became the most frequently cited ones during the decade of 1981-1990 and former Associate Director of Science for the Office of Basic Energy Sciences Patricia M. Dehmer presented our work as one of the great successes of this program. A major component of the sequencing strategy was the development of bacterial strains and vectors, which were also used to develop the first biotechnology crops. These crops possessed new traits thanks to the expression of foreign genes in plants. To enable such expression, chimeric genes had to be constructed using our materials and methods by the industry. Because we made our materials and methods freely available to

  17. Genetic markers for western corn rootworm resistance to Bt toxin.

    Science.gov (United States)

    Flagel, Lex E; Swarup, Shilpa; Chen, Mao; Bauer, Christopher; Wanjugi, Humphrey; Carroll, Matthew; Hill, Patrick; Tuscan, Meghan; Bansal, Raman; Flannagan, Ronald; Clark, Thomas L; Michel, Andrew P; Head, Graham P; Goldman, Barry S

    2015-01-07

    Western corn rootworm (WCR) is a major maize (Zea mays L.) pest leading to annual economic losses of more than 1 billion dollars in the United States. Transgenic maize expressing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are widely used for the management of WCR. However, cultivation of Bt-expressing maize places intense selection pressure on pest populations to evolve resistance. Instances of resistance to Bt toxins have been reported in WCR. Developing genetic markers for resistance will help in characterizing the extent of existing issues, predicting where future field failures may occur, improving insect resistance management strategies, and in designing and sustainably implementing forthcoming WCR control products. Here, we discover and validate genetic markers in WCR that are associated with resistance to the Cry3Bb1 Bt toxin. A field-derived WCR population known to be resistant to the Cry3Bb1 Bt toxin was used to generate a genetic map and to identify a genomic region associated with Cry3Bb1 resistance. Our results indicate that resistance is inherited in a nearly recessive manner and associated with a single autosomal linkage group. Markers tightly linked with resistance were validated using WCR populations collected from Cry3Bb1 maize fields showing significant WCR damage from across the US Corn Belt. Two markers were found to be correlated with both diet (R2 = 0.14) and plant (R2 = 0.23) bioassays for resistance. These results will assist in assessing resistance risk for different WCR populations, and can be used to improve insect resistance management strategies.

  18. The inlfuence of potassium to mineral fertilizers on the maize health

    Institute of Scientific and Technical Information of China (English)

    Jan Bocianowski; Piotr Szulc; Anna Tratwal; Kamila Nowosad; Dariusz Piesik

    2016-01-01

    Field experiments (2009–2011) were conducted at the Department of Agronomy at Poznań University of Life Sciences on the ifelds of the Research Institute in Swadzim. We evaluated the health of maize plants of two types, depending on the variations in mineral fertilization. The conducted research recorded the occurrence of pests such as oscinela frit (Oscinela fritL.) and the European corn borer (Pyrausta nubilalis Hbn.). Diseases recorded during the research included two patho-genes:Fusarium (Fusarium ssp.) and corn smut (Ustilago maydis Corda). It was shown that the meteorological conditions during the maize vegetation had a signiifcant inlfuence on the occurrence of pests. Adding potassium to mineral fertilizers increased the maize resistance toFusarium. Cultivation of “stay-green” cultivar shal be considered as an element of in-tegrated maize protection. The occurrence of oscinela frit was correlated with the occurrence ofFusarium as wel as the occurrence of the European corn borer for both examined cultivars.

  19. MaizeGDB, the community database for maize genetics and genomics

    OpenAIRE

    2004-01-01

    The Maize Genetics and Genomics Database (MaizeGDB) is a central repository for maize sequence, stock, phenotype, genotypic and karyotypic variation, and chromosomal mapping data. In addition, MaizeGDB provides contact information for over 2400 maize cooperative researchers, facilitating interactions between members of the rapidly expanding maize community. MaizeGDB represents the synthesis of all data available previously from ZmDB and from MaizeDB—databases that have been superseded by Maiz...

  20. Allelopathic effects of barley straw on germination and seedling growth of corn, sugar beet and sunflower

    Directory of Open Access Journals (Sweden)

    mohamad taghi naseri poor yazdi

    2009-06-01

    Full Text Available Allelopathic effects of barley straw and root on germination and growth of maize, sugar beet, and sunflower were investigated under glasshouse and laboratory experiments in Faculty of Agriculture, Ferdowsi University of Mashhad in 2006. The glasshouse experiment was designed based on randomized complete block design with three replications, treatments included: 0, 200, 400, 600 g/m² of grounded barley straw and also 0 and 50 g/m2 barley root. A laboratory experiment was carried out in order to study the effect of different concentrations of barley water extracts on germination and seedling characteristics of corn, sugar beet and sunflower. Treatments in laboratory trial included 0, 33, 50 and 100 percent of barley extracts. Results showed that leaf area of corn was significantly affected by barley straw treatments. Shoot dry matter and seed weight per plant in corn , leaf and tuber weight in sugar beet and leaf , stem weights , plant per plant in corn , leaf and tuber weight in sugar beet and leaf, stem weights, plant height, head diameter, head weight and seed weight in sunflower were significantly higher in treatment of 50g/m² barley roots. Crop seed germination decreased with increasing the amount of barley straw. The best germination response to barley extract was observed in corn. Maize radicle weight was significantly decreased with increasing concentration of barley water extract.

  1. Effectiveness of spinosad against seven major storedgrain insects on corn

    Institute of Scientific and Technical Information of China (English)

    FANGNENG HUANG; BHADRIRAJU SUBRAMANYAM

    2007-01-01

    In January 2005, the United States Environmental Protection Agency registered spinosad as a stored grain protectant. No referenced data on the efficacy of spinosad on corn in suppressing major stored-grain insects have been published. In this paper, we evaluated the efficacy of spinosad against seven major stored-grain insects on shelled corn in the laboratory. Insect species tested were the red flour beetle, Tribolium castaneum (Jacquelin duVal); rusty grain beetle, Cryptolestes ferrugineus (Stephens); lesser grain borer, Rhyzopertha dominica (F.); sawtoothed grain beetle, Oryzaephilus surinamensis (L.); rice weevil, Sitophilus oryzae (L.); maize weevil, Sitophilus zeamais (Motschulsky); and Indian meal moth, Plodia interpunctella (Hübner). Corn kernels were treated with spinosad at 0,0.1, 0.5, 1, and 2 active ingredient (a.i.) mg/kg for controlling the seven species. Beetle adults or P.interpunctella eggs were introduced into each container holding 100 g of untreated or insecticide-treated corn. The seven insect species survived well on the control treatment, produced 28 to 336 progeny, and caused significant kernel damage after 49 days. On spinosad-treated corn, adult mortality of C. ferrugineus, R. dominica, O. surinamensis, S. oryzae, and S. zeamais was > 98% at 1 and 2 mg/kg after 12 days. Spinosad at > 0.5 mg/kg completely suppressed egg-to-larval survival after 21 days and egg-to-adult emergence of P. interpunctella after 49 days, whereas 16% T. castaneum adults survived at 1 mg/kg after 12 days. Spinosad at 1 or 2 mg/kg provided complete or near complete suppression of progeny production and kernel damage of all species after 49 days. Our results indicate that spinosad at the current labeled rate of 1 mg/kg is effective against the seven stored-grain insect pests on corn.

  2. Production and Quality Evaluation of Soy-Corn Yoghurt

    Directory of Open Access Journals (Sweden)

    Olakunle Moses Makanjuola

    2012-06-01

    Full Text Available In the recent years, research efforts in the developing countries have been geared towards the improvement of protein quality foods using blends of legume and cereal which is considered a nutritionally balanced product. Therefore, this present study is aimed at finding local substitute for milk based product with high protein content of a well balancing amino acid composition and high digestibility; determining the proximate composition, microbial analysis as well as sensory evaluation of soy-corn yoghurt with a view of knowing the consumer acceptability of the product. Yoghurt samples were produced from blends of soymilk and corn milk (yellow maize corn using Streptococcus thermophillus and Lactobacillus bulgaricus as starter cultures. Ratio of soy milk to corn milk were 80%:20%; 70%:30% and 100% soymilk as control. The yoghurt samples produced were coded A, B and C representing 100% soy yoghurt, 80%:20% soy corn yoghurt and 70%:30% soy corn yoghurt respectively. Yoghurt samples were subjected to chemical, microbiological and organoleptic assessment. The results of chemical analysis revealed protein contents of 4.30, 4.00 and 3.70% respectively for the samples. Fat contents varied between 2.10 and 2.60%, while ash contents of 0.50, 0.60 and 0.62% were obtained for the samples respectively. Total solids of between 10.98 and 8.80% were obtained with titratable acidity of 0.03, 0.05 and 0.06%, respectively. The carbohydrate contents of the samples ranged from 1.40 to 4.50% while all the samples showed fairly acidic levels. Water contents of between 89.00 and 91.20% were obtained. The microbiological examination revealed a tolerable level for all the samples.

  3. Modification of lipid fraction in ensiled high moisture corn

    Directory of Open Access Journals (Sweden)

    Davide Bochicchio

    2015-09-01

    Full Text Available The aim of this work is to study the changes of the lipidic fraction of ensiled high moisture corn (HMC. 11 maize hybrids were used, ensiled each in 3 mini experimental silos of 100 litres. For each hybrid 1 sample of fresh high moisture corn was obtained immediately after milling and 3 samples of ensiling HMC were kept after 2, 7 and 12 months. All samples were analysed for pH, dry matter, lactic acid, ammonia-N, ether extract, fatty acid composition and volatile fatty acids (VFAs. Ether extract of fresh high moisture corn was 35.7 g/kg dry matter (DM and increased after 2 and 7 months of storage up to 39.4 g/Kg DM (P≤0.01; after 12 months it decreased to 38.1 g/kg DM (P≤0.01. Both saturated fatty acids (SFA and monounsaturated fatty acids (MUFA percentages decreased from 18.23% to 15.47% and from 24.84% to 23.57% respectively (before ensiling vs 12 months P≤0.01. Linoleic acid percentage increased from 55.34% to 59.44% (before ensiling vs 12 months P≤0.01. The linoleic acid content (g/kg of DM increased on average from 19,1 before ensiling to 22.5 after 12 months of ensiling. These differences may affect the linoleic acid content of heavy pig diets when maize is used as HMC instead of corn meal.

  4. High susceptibility of Bt maize to aphids enhances the performance of parasitoids of lepidopteran pests.

    Directory of Open Access Journals (Sweden)

    Cristina A Faria

    Full Text Available Concerns about possible undesired environmental effects of transgenic crops have prompted numerous evaluations of such crops. So-called Bt crops receive particular attention because they carry bacteria-derived genes coding for insecticidal proteins that might negatively affect non-target arthropods. Here we show a remarkable positive effect of Bt maize on the performance of the corn leaf aphid Rhopalosiphum maidis, which in turn enhanced the performance of parasitic wasps that feed on aphid honeydew. Within five out of six pairs that were evaluated, transgenic maize lines were significantly more susceptible to aphids than their near-isogenic equivalents, with the remaining pair being equally susceptible. The aphids feed from the phloem sieve element content and analyses of this sap in selected maize lines revealed marginally, but significantly higher amino acid levels in Bt maize, which might partially explain the observed increased aphid performance. Larger colony densities of aphids on Bt plants resulted in an increased production of honeydew that can be used as food by beneficial insects. Indeed, Cotesia marginiventris, a parasitoid of lepidopteran pests, lived longer and parasitized more pest caterpillars in the presence of aphid-infested Bt maize than in the presence of aphid-infested isogenic maize. Hence, depending on aphid pest thresholds, the observed increased susceptibility of Bt maize to aphids may be either a welcome or an undesirable side effect.

  5. Comparison of Phosphorous Absorption, Quality and Yield Between High Oil Corn and Common Corn as Influenced by Phosphorous Application

    Institute of Scientific and Technical Information of China (English)

    HE Ping; JIN Ji-yun; LI Wen-juan; LIU Hai-long; HUANG Shao-wen; WANG Xiu-fang; WANG Li-chun; XIE Jia-gui

    2005-01-01

    A field trial was carried out to investigate phosphorous (P) absorption, grain quality and yield between high oil corn and common corn. The results indicated that high oil corn var. Tongyou 1 obtained lower highest P absorption rate (HAR) and later occurring date of HAR, in comparison with common corn var. Simi 25. The highest HAR and the earliest occurring date of HAR was obtained by the treatments of P45 and P75 in Tongyou 1 and Simi 25 separately; while the total amount of P accumulated by maize plant was achieved by P105 treatment in both varieties. P in grain relied mainly on root uptake at maturation that accounted for 85.7-96.8% and 79.3-84.3% for Tongyou 1 and Simi 25, respectively. Tongyou 1 contained more oil and protein contents, but less starch content with lower grain yield. P application at appropriate rate enhanced contents of protein and fatty acid, but the increment of starch content was neglectable.

  6. Integrated systems of producing feed and ethanol from fractionated maize silage

    Energy Technology Data Exchange (ETDEWEB)

    Ganesh, D.; Mowat, D.N.

    1985-01-01

    Systems were designed, simulated and analyzed to assess the economic feasibility of producing ethanol from high-moisture grain, gradually separated from mature whole-plant maize silage. The residual stover fraction containing some grain fines was fed, along with ethanol production by-products (stillage or maize gluten feed), to growing steers. Three systems were compared. In the control, regular maize silage was fed to growing steers with extra maize harvested later and sold as grain for cash. In one alternative (system 2), the separated grain fraction was processed to ethanol and stillage at a local farmer-cooperative plant. In another alternative (system 3), the grain fraction was transferred to a regional industrial plant for wet milling to ethanol, corn gluten-feed and other products. System comparisons were based on estimating gross costs per farm during 1980 to 1982, minus credits for products such as grain maize (control) and ethanol (alternative systems). System 3 was the more attractive alternative. When ethanol was valued at wholesale prices for regular leaded gasoline, these costs were similar in 1981 and 1982 for System 3 and the control. Further refinements of a separation unit, and detailed assessment of the feeding value of the stover fraction plus stillage or corn gluten feed, are warranted. 17 references.

  7. Maize microarray annotation database

    Directory of Open Access Journals (Sweden)

    Berger Dave K

    2011-10-01

    Full Text Available Abstract Background Microarray technology has matured over the past fifteen years into a cost-effective solution with established data analysis protocols for global gene expression profiling. The Agilent-016047 maize 44 K microarray was custom-designed from EST sequences, but only reporter sequences with EST accession numbers are publicly available. The following information is lacking: (a reporter - gene model match, (b number of reporters per gene model, (c potential for cross hybridization, (d sense/antisense orientation of reporters, (e position of reporter on B73 genome sequence (for eQTL studies, and (f functional annotations of genes represented by reporters. To address this, we developed a strategy to annotate the Agilent-016047 maize microarray, and built a publicly accessible annotation database. Description Genomic annotation of the 42,034 reporters on the Agilent-016047 maize microarray was based on BLASTN results of the 60-mer reporter sequences and their corresponding ESTs against the maize B73 RefGen v2 "Working Gene Set" (WGS predicted transcripts and the genome sequence. The agreement between the EST, WGS transcript and gDNA BLASTN results were used to assign the reporters into six genomic annotation groups. These annotation groups were: (i "annotation by sense gene model" (23,668 reporters, (ii "annotation by antisense gene model" (4,330; (iii "annotation by gDNA" without a WGS transcript hit (1,549; (iv "annotation by EST", in which case the EST from which the reporter was designed, but not the reporter itself, has a WGS transcript hit (3,390; (v "ambiguous annotation" (2,608; and (vi "inconclusive annotation" (6,489. Functional annotations of reporters were obtained by BLASTX and Blast2GO analysis of corresponding WGS transcripts against GenBank. The annotations are available in the Maize Microarray Annotation Database http://MaizeArrayAnnot.bi.up.ac.za/, as well as through a GBrowse annotation file that can be uploaded to

  8. Translocation of the neonicotinoid seed treatment clothianidin in maize

    Science.gov (United States)

    Krupke, Christian H.

    2017-01-01

    Neonicotinoid seed treatments, typically clothianidin or thiamethoxam, are routinely applied to >80% of maize (corn) seed grown in North America where they are marketed as a targeted pesticide delivery system. Despite this widespread use, the amount of compound translocated into plant tissue from the initial seed treatment to provide protection has not been reported. Our two year field study compared concentrations of clothianidin seed treatments in maize to that of maize without neonicotinoid seed treatments and found neonicotinoids present in root tissues up to 34 days post planting. Plant-bound clothianidin concentrations followed an exponential decay pattern with initially high values followed by a rapid decrease within the first ~20 days post planting. A maximum of 1.34% of the initial seed treatment was successfully recovered from plant tissues in both study years and a maximum of 0.26% was recovered from root tissue. Our findings show neonicotinoid seed treatments may provide protection from some early season secondary maize pests. However, the proportion of the neonicotinoid seed treatment clothianidin translocated into plant tissues throughout the growing season is low overall and this observation may provide a mechanism to explain reports of inconsistent efficacy of this pest management approach and increasing detections of environmental neonicotinoids. PMID:28282441

  9. The maize cystatin CC9 interacts with apoplastic cysteine proteases.

    Science.gov (United States)

    van der Linde, Karina; Mueller, André N; Hemetsberger, Christoph; Kashani, Farnusch; van der Hoorn, Renier A L; Doehlemann, Gunther

    2012-11-01

    In a recent study we identified corn cystain9 (CC9) as a novel compatibility factor for the interaction of the biotrophic smut fungus Ustilago maydis with its host plant maize. CC9 is transcriptionally induced during the compatible interaction with U. maydis and localizes in the maize apoplast where it inhibits apoplastic papain-like cysteine proteases. The proteases are activated during incompatible interaction and salicylic acid (SA) treatment and, in turn, are sufficient to induce SA signaling including PR-gene expression. Therefore the inhibition of apoplastic papain-like cysteine proteases by CC9 is essential to suppress host immunity during U. maydis infection. Here were present new experimental data on the cysteine protease-cystatin interaction and provide an in silco analysis of plant cystatins and the identified apoplastic cysteine proteases.

  10. Preliminary Trial of 11 New Hybrid Maize Genotype to The Resistance on Java Downy Mildew (Peronosclerospora maydis

    Directory of Open Access Journals (Sweden)

    Budi Setyawan

    2016-04-01

    Full Text Available Maize or corn (Zea mays L. belongs to the family of grasses (Poaceae.  Maize is grown globally and one of the most important cereal crop in the world.  In many countries, corn is the main agricultural crop, and are used as food, feed and industrial raw materials. Together with rice and wheat, corn included in the cereals that provide about 65% carbohydrates and 50% protein that humans need. For this purpose, many developing countries, especially in Asia and Africa are in a strong effort to increase their corn yields through the use of better seeds.  Although in Indonesia, corn is the second important food crop after rice, however, with the rapid growth of the livestock industry, corn is a major component (60% in feed ingredient.  It is estimated that more than 55% of the corn used for feed in Indonesia.  Java Downy Mildew (Peronosclerospora maydis is the main disease that is concerned by maize corn growers. This disease often resulting in substantial losses for farmers, even reach 100% in susceptible genotypes.  Therefore trial on 11 new prospective hybrids was conducted with the expectation that they can be classified in the criteria “less resistant or higher” according to the criteria of modified Reid (2005.  Of the 11 new hybrids, 3 new hybrids (27.3% classified in the criteria “very resistant”, 4 new hybrids (36.4% classified in the criteria “resistant”, 2 new hybrid (18.2% classified in the criteria “less resistant”, and 2 other new hybrids (18.2% classified in the criteria “less susceptible”.

  11. BIOFUEL FROM CORN STOVER

    Directory of Open Access Journals (Sweden)

    Ljiljanka Tomerlin

    2003-12-01

    Full Text Available This paper deals with production of ethyl alcohol (biofuel from corn stover acid hydrolysate by yeasts, respectively at Pichia stipitis y-7124 and Pachysolen tannophilus y-2460 and Candida shehatae y-12856. Since moist corn stover (Hybryds 619 is proving to decomposition by phyllospheric microflora. It was (conserved spattered individually by microbicids: Busan-90, Izosan-G and formalin. In form of prismatic bales, it was left in the open air during 6 months (Octobar - March. At the beginning and after 6 months the microbiological control was carried out. The only one unspattered (control and three stover corn bals being individually spattered by microbicids were fragmented and cooked with sulfur acid. The obtained four acid hydrolysates are complex substratums, containing, apart from the sugars (about 11 g dm-3 pentosa and about 5.4 g dm-3 hexose, decomposite components as lignin, caramel sugars and uronic acids. By controlling the activity of the mentioned yeasts it was confirmed that yeasts Pichia stipitis y-7124 obtained best capability of ethyl alcohol production from corn stover acid hydrolysate at 0.23 vol. % to 0.49 vol. %.

  12. Breeding of speciality maize for industrial purposes

    OpenAIRE

    2010-01-01

    The breeding programme on speciality maize with specific traits was established at the Maize Research Institute, Zemun Polje, several decades ago. The initial material was collected, new methods applying to breeding of speciality maize, i.e. popping maize, sweet maize and white-seeded maize, were introduced. The aim was to enhance and improve variability of the initial material for breeding these three types of maize. Then, inbred lines of good combining abilities were developed and used as c...

  13. Quality protein maize: QPM

    Directory of Open Access Journals (Sweden)

    Ignjatović-Micić Dragana

    2008-01-01

    Full Text Available Quality protein maize (QPM contains the opaque-2 gene along with numerous modifiers for kernel hardness. Therefore, QPM is maize with high nutritive value of endosperm protein, with substantially higher content of two essential amino acids - lysine and tryptophan, and with good agronomical performances. Although QPM was developed primarily for utilization in the regions where, because of poverty, maize is the main staple food, it has many advantages for production and consumption in other parts of the world, too. QPM can be used for production of conventional and new animal feed, as well as for human nurture. As the rate of animal weight gain is doubled with QPM and portion viability is better, a part of normal maize production could be available for other purposes, such as, for example, ethanol production. Thus, breeding QPM is set as a challenge to produce high quality protein maize with high yield and other important agronomical traits, especially with today's food and feed demands and significance of energy crisis.

  14. Acquisition of Insect-Resistant Transgenic Maize Harboring a Truncated cry1Ah Gene via Agrobacterium-Mediated Transformation

    Institute of Scientific and Technical Information of China (English)

    LI Xiu-ying; LANG Zhi-hong; ZHANG Jie; HE Kang-lai; ZHU Li; HUANG Da-fang

    2014-01-01

    A novel insecticidal gene cry1Ah was cloned from Bacillus thuringiensis isolate BT8 previously for plant genetic engineering improvement. Truncated active Cry1Ah toxin has a toxicity level similar to that of the full-length Cry1Ah toxin. In this study, plant expression vector pMhGM harboring truncated cry1Ah gene was transformed into maize (Zea mays L.) immature embryos by Agrobacterium tumefaciens-mediated transformation at which maize alcohol dehydrogenase matrix attachment regions (madMARs) were incorporated on both sides of the gene expression cassette to improve gene expression. A total of 23 PCR positive events were obtained with a transformation efifciency of 5%around. Bioassay results showed that events 1-4 and 1-5 exhibited enhanced resistance to the Asian corn borer (Ostrinia furnacalis). These two events were further conifrmed by molecular analysis. Southern blot suggested that a single copy of the cry1Ah gene was successfully integrated into the maize genome. Western blot and ELISA showed that the foreign gene cry1Ah was expressed stably at high level in maize and could be inherited stably over generations. The results of a bioassay of T1-T4 transgenic maize plants indicated that the transgenic plants were highly toxic to the Asian corn borer and their resistance could be inherited stably from generation to generation. Thus, events 1-4 and 1-5 are good candidates for the breeding of insect-resistant maize.

  15. Successful application of entomopathogenic nematodes for the biological control of western corn rootworm larvae in Europe – a mini review

    Directory of Open Access Journals (Sweden)

    Toepfer, Stefan

    2014-02-01

    Full Text Available 10 years of joint efforts in research and development have led to a nematode-based biological control solution for one of the most destructive maize pests, the western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae. Commercially mass-produced Heterorhabditis species of beneficial entomopathogenic nematodes are ready to use. They can be applied into the soil during sowing of maize for controlling the subsequently hatching larvae of D. virgifera virgifera thus preventing root feeding and damage to maize. Policy bodies, decision makers and farmers are advised to consider biological control as one of the alternatives to synthetic pesticides in maize production, and according to the EC Directive on the sustainable use of pesticides and implementation of integrated pest management.

  16. Analysis of Maize versus Ethanol Production in Nebraska, United States and International Agricultural Droughts: Lessons for Global Food Security

    Science.gov (United States)

    Boken, V.; Tenkorang, F.

    2012-04-01

    Nebraska is one of the eight main corn (maize) belt states of the United States. Maize is the major crop of Nebraska with an average annual production of about 38 million tons (about 12% of U.S. production), which contributes billions of dollars to the state's economy. The yield of maize has increased significantly over the past century - from 1.6 t/ha in 1900 to 10.4 t/ha in 2010. While the majority of maize (about 40%) is currently used for animal feed and ethanol production, only about six percent is exported. It is estimated that about one billion people accounting for about 15% population of the world live in chronic hunger because of low agricultural productivity and drought. Most of these people depend on the U.S. for grains including maize. If a greater quantity of maize is diverted to ethanol production, considerably less quantity of maize would be available for export to developing countries where it could be used for human consumption and to mitigate hunger and improve food security. This paper presents analysis of maize production in Nebraska for the past three decades and examines how its commercialization for ethanol production has affected its exports in the face of drought at an international level.

  17. Ultrarapid quantitation of maize proteins by perfusion and monolithic reversed-phase high-performance liquid chromatography.

    Science.gov (United States)

    Rodríguez-Nogales, J M; del Alamo, M; García, M C; Cifuentes, A; Marina, M L

    2009-04-22

    The main objective of this study was to develop a new methodology alternative to the classical Kjeldahl analysis for determining maize proteins in maize products and seeds. For that purpose, two different chromatographic methodologies using perfusion and monolithic stationary phases, both enabling rapid separations of maize proteins, were investigated. Due to the difficulty to find suitable standards for this type of analysis, three different maize products were initially tested as proteins standards: zein F4000, corn gluten meal, and maize flour. Different figures of merit (i.e., linearity, correlation coefficient, precision, limits of detection and quantitation), as well as the presence of matrix inferences, were investigated. The results obtained for the different chromatographic stationary phases and protein standards were compared in order to select the most suitable analytical conditions. Despite both perfusion and monolithic methodologies resulting, in general, as appropriate for the quantitation of maize proteins, the highest reduction of analysis time and lowest detection and determination limits provided by perfusion methodology enabled to select this one as the method of choice for the quantitation of maize proteins. Regarding the different protein standards studied in this work, in general the best results were obtained using the zein standard. Compared to Kjeldahl methodology, perfusion chromatography yields total protein contents in shorter analysis time while enabling the separation of the different kinds of proteins. Due to the high diversity and complexity of industrial maize products, the proposed chromatographic method could be a very useful tool for their routine analysis.

  18. Glycaemic Response to Quality Protein Maize Grits

    Directory of Open Access Journals (Sweden)

    Leonora N. Panlasigui

    2010-01-01

    Full Text Available Background. Carbohydrates have varied rates of digestion and absorption that induces different hormonal and metabolic responses in the body. Given the abundance of carbohydrate sources in the Philippines, the determination of the glycaemic index (GI of local foods may prove beneficial in promoting health and decreasing the risk of diabetes in the country. Methods. The GI of Quality Protein Maize (QPM grits, milled rice, and the mixture of these two food items were determined in ten female subjects. Using a randomized crossover design, the control bread and three test foods were given on separate occasions after an overnight fast. Blood samples were collected through finger prick at time intervals of 0, 15, 30, 45, 60, 90, and 120 min and analyzed for glucose concentrations. Results. The computed incremental area under the glucose response curve (IAUC varies significantly across test foods (P<.0379 with the pure QPM grits yielding the lowest IAUC relative to the control by 46.38. Resulting GI values of the test foods (bootstrapped were 80.36 (SEM 14.24, 119.78 (SEM 18.81, and 93.17 (SEM 27.27 for pure QPM grits, milled rice, and rice-QPM grits mixture, respectively. Conclusion. Pure QPM corn grits has a lower glycaemic response compared to milled rice and the rice-corn grits mixture, which may be related in part to differences in their dietary fibre composition and physicochemical characteristics. Pure QPM corn grits may be a more health beneficial food for diabetic and hyperlipidemic individuals.

  19. CYCLOXYDIM-TOLERANT MAIZE – BREEDERS STANDPOINT

    Directory of Open Access Journals (Sweden)

    G. Bekavac

    2008-09-01

    Full Text Available Cycloxydim-Tolerant Maize (CTM was developed by researches at the University of Minnesota. CTM plants were regenerated from tissue culture selected for callus growth in the presence of cycloxydim, and the resulting plants were shown to contain a nuclear mutation, expressed as a single, partially dominant gene (known as Acc1 that conferred tolerance to the herbicide. Cycloxydim is a systemic herbicide for post emergence application in dicot crops to selectively control grass weeds. Corn, like most grasses is susceptible to cycloxydim due to inhibited acetyl-coenzyimeA carboxylaze enzyme activity. There are two key benefits of this technology: first, cycloxydim applications in CTM hybrids can be delayed until the weed spectrum and population density exceed agro-economic threshold; second, cycloxydim can be applied at either stage of plant development with no effect on basic agronomic traits, compared to non treated plants. Nevertheless, this type of tolerance requires 2 genes to be fully effective, i.e. gene must be present in both inbred parents to provide complete tolerance in the resulting hybrid. Such type of tolerance doubles the chances for yield drag and doubles the number of inbred conversions needed. This also limits germplasm integration and increases time lag in developing hybrids. Despite these difficulties, many seed companies introduce tolerance to cycloxydim into their commercial inbreds, and many of them have already commercialized CTM hybrids. Finally, it came as a logical question what is more important – hybrid performance or new trait? Critical to the success of this technology has been yield performance of CTM hybrids. At the same time, performance and herbicide tolerance do not exclude each other and can surely co-exist. To be accepted, this coexistence must secure high profitability to corn producers. However, CTM hybrids will not replace conventional ones on a large scale, but could be used as a specific tool, or could

  20. Breeding of maize types with specific traits at the Maize Research Institute, Zemun Polje

    OpenAIRE

    2007-01-01

    Maize is primarily grown as an energy crop, but the use of different specific versions, such as high-oil maize, high-lysine maize, waxy maize, white-seeded maize, popping maize and sweet maize, is quite extensive. Speciality maize, due to its traits and genetic control of these traits, requires a particular attention in handling breeding material during the processes of breeding. It is especially related to prevention of uncontrolled pollination. In order to provide successful selection for a...

  1. RELATIONSHIP BETWEEN EUROPEAN CORN BORER FEEDING ACTIVITY AND NITROGEN LEAF CONTENT UNDER DIFFERENT AGRICULTURAL PRACTICES

    Directory of Open Access Journals (Sweden)

    Ankica Sarajlić

    2015-06-01

    Full Text Available One of the most destructive maize pest in Croatia is European corn borer (Ostrinia nubilalis Hübner (ECB. The aim of this study was to determine the influence of irrigation, nitrogen fertilization, different maize genotypes and nitrogen leaf content on ECB feeding activity. The experiment was set up in Osijek, Croatia under field conditions during 2012-2013 vegetation season. Experiment treatments were as follows: three irrigation levels (A1 - control, A2 from 60% to 80% field water capacity - FWC and A3 from 80% to100% FWC, three nitrogen fertilizer levels (B1 - 0, B2 - 100 and B3 - 200 kg N/ha and four different genotypes (C1 - OSSK 596; C2 - OSSK 617; C3 - OSSK 602 and C4 - OSSK 552. Ear weight, number of larvae in stem and shank, tunnel length and nitrogen leaf content were evaluated. Genotype C1 was the most susceptible for following the tested variables of ECB feeding: tunnel length (TL, larvae in stalk (LS and total number of larvae (TNL at P<0.05 probability level. By raising the level of irrigation, European corn borer feeding activity was reduced while by raising the level of nitrogen fertilization feeding activity was increased. These results suggest that good production practices can significantly affect the susceptibility of maize to European corn borer.

  2. 21 CFR 184.1321 - Corn gluten.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Corn gluten. 184.1321 Section 184.1321 Food and... Substances Affirmed as GRAS § 184.1321 Corn gluten. (a) Corn gluten (CAS Reg. No. 66071-96-3), also known as corn gluten meal, is the principal protein component of corn endosperm. It consists mainly of zein...

  3. Pyramiding the disease resistant genes to southern rust and stalk rot in maize(Zea mays L.) with marker-assisted selection

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Southern corn rust(SCR) caused by Puccinia polysora Underw and maize stalk rot caused by Pythium inflatum Matthews(MSR-2) are two destructive diseases of maize(Zea mays L.) in China.Our previous studies indicated that maize inbred line Qi319 is highly resistant to SCR but susceptible to MSR-2,while inbred line 1145 is highly resistant to MSR-2 but susceptible to SCR.The SCR resistant gene(RppQ) in Qi319 and MSR-2 resistant gene(Rpi1) in 1145 have been mapped on chromosome 10 and 4 respectively.In this resea...

  4. Changes in phenolic concentrations during recurrent selection for resistance to the Mediterranean corn borer (Sesamia nonagrioides Lef.).

    Science.gov (United States)

    Santiago, Rogelio; Sandoya, German; Butrón, Ana; Barros, Jaime; Malvar, Rosa A

    2008-09-10

    Recurrent selection has been reported as successful for improving maize resistance against corn borers. This study was conducted to determine if phenolics concentration in maize changes during recurrent selection to improve stalk resistance to the Mediterranean corn borer. Three cycles of selection [EPS12(S)C0, ESP12(S)C2, and EPS12(S)C3] from the maize synthetic population EPS12 and test crosses to inbred lines A639, B93, and EP42 were field grown and artificially infested with Mediterranean corn borer larvae, and the pith tissues were sampled for biochemical analyses. Two major simple phenolic acids [p-coumaric (p-CA) and trans-ferulic (E-FA) acids] were identified in free and cell-wall fractions, whereas four isomers of diferulic acid (DFA) (8-5'l, 5-5', 8-o-4', and 8-5' benzofuran form) were present in the cell-wall bound fraction. The selection cycles EPS12(S)C0 and EPS12(S)C3 showed less damage and higher cell wall phenolics concentrations than the cycle EPS12(S)C2. In addition, higher concentrations of total DFAs were associated with shorter tunnel length and lower numbers of larvae per stem. The current study shows new and concrete evidence that the cell-wall bound phenolics could have a determinative role in the resistance to the Mediterranean corn borer, although future development of recurrent and divergent selection cycles will clarify this point.

  5. Relationship between time to flowering and stalk and ear damage by second generation corn borers.

    Science.gov (United States)

    Ordas, B; Alvarez, A; Revilla, P; Butron, A; Malvar, R A

    2013-06-01

    In the Mediterranean area, the main corn borer species are Sesamia nonagrioides Lefebvre (Mediterranean corn borer) and Ostrinia nubilalis Hübner (European corn borer). In the overall context of integrated pest control, it is possible to reduce the effect of a pest without having a negative effect on the environment by varying the sowing date. Benefits are possible if the most susceptible stages of the crop no longer coincide with the peak of the pest. We used different cycles of selection (0, 6, 8, 10, and 12) of two populations (Purdue A and Purdue B) of maize selected for early flowering to get a more precise estimation of the relationship between maturity of plant tissues and corn borer damage. We found a relationship between the damage produced by corn borers and the number of days from flowering to infestation. We conclude that, after flowering, a later stage of plant development at the moment of the infestation by corn borers reduces the damage caused by the larvae. Based on our results, we recommend to plant as early as possible so the tissues would be as mature as possible at the moment of insect attack.

  6. The Effects of Nitrogen Source on Nutritive Value of Irrigated Silage Corn

    Directory of Open Access Journals (Sweden)

    Seyfollah FALLAH

    2017-03-01

    Full Text Available Nitrogen is considered one of the most important nutrients affecting yield and quality of maize forage (Zea mays L.. A two-year field experiment was carried out to evaluate the effects of broiler litter and mineral fertilizer on dry matter production and silage quality of corn. The applied treatments were: unfertilized (control, 100, 200 and 300 kg ha-1 in the form of urea fertilizer (217, 434, and 651 kg urea ha-1, respectively and 100, 200 and 300 kg ha-1 in the form of broiler litter (7142, 14284, and 21426 kg broiler litter ha-1, respectively. The present findings showed that Fe, Mn, Cu and Zn concentrations in forage corn were not affected by urea application; however, broiler litter application significantly increased Fe, Mn and Cu concentrations of corn stover in a linear trend. The broiler litter and urea fertilizer significantly increased both dry matter and protein content of forage corn but no significant differences on those components were obtained between broiler litter and urea fertilizer at each N application rate. The results suggested that N applied to forage corn by broiler litter, at the recommended rate for inorganic N fertilization, is almost more effective in terms of forage nutritive value response than urea fertilizer. The profitability of forage corn production could be enhanced by replacing chemical fertilizers with broiler litter.

  7. Recrystallization characteristics of high hydrostatic pressure gelatinized normal and waxy corn starch.

    Science.gov (United States)

    Li, Wenhao; Tian, Xiaoling; Wang, Peng; Saleh, Ahmed S M; Luo, Qingui; Zheng, Jianmei; Ouyang, Shaohui; Zhang, Guoquan

    2016-02-01

    High hydrostatic pressure (HHP) can lead to starch gelatinization at room temperature, while the retrogradation mechanism of HHP gelatinized starch is not well known. HHP gelatinized normal and waxy corn starches were stored at room temperature for 192 h in order to investigate the retrogradation characteristics. The scanning electron microscopy (SEM), polarised light microscopy and differential scanning calorimetric (DSC) analysis showed that the pressurization of normal and waxy corn starch suspensions with concentration of 30% (w/v) at 600 MPa for 15 min resulted in a complete gelatinization. In addition, the pressure-gelatinized normal and waxy corn starch gels were stored and subjected to X-ray diffraction (XRD) analysis, resistant starch content determination, swelling power and pasting behavior. The retrograded normal maize and waxy maize starch showed a substantial loss of A-type crystallinity. Both pressure-gelatinized normal and waxy corn starches showed an increase in resistant starch content and relative crystallinity degree with the increase of storage time. In addition, restricted starch swelling power and lower pasting viscosities were observed for these two retrograded starches. The amylose molecule within starch granules has been regarded as the main factor to affect the structural and physicochemical properties during the retrogradation process of HHP-gelatinized starch granules.

  8. Radiological study on newly developed composite corn advance lines in Malaysia

    Science.gov (United States)

    Adekunle Olatunji, Michael; Bemigho Uwatse, Onosohwo; Uddin Khandaker, Mayeen; Amin, Y. M.; Faruq, G.

    2014-12-01

    Owing to population growth, there has been high demand for food across the world, and hence, different agricultural activities such as use of phosphate fertilizers, recycling of organic matters, etc, have been deployed to increase crop yields. In Malaysia, a total of nine composite corn advance lines have been developed at the Institute of Biological Sciences, University of Malaya and are being grown under different conditions with a bid to meet the average daily human need for energy and fiber intake. To this end, the knowledge of radioactivity levels in these corn advance lines are of paramount importance for the estimation of possible radiological hazards due to its consumption. Hence, the radioactivity concentrations of 226Ra, 228Ra and 40K in the corn have been determined using HPGe γ-ray spectrometry. The activity concentrations in the corn ranged from 0.05 to 19.18 Bq kg-1 for 226Ra, from 0.10 to 3.22 Bq kg-1 for 228Ra and from 26.4 to 129 Bq kg-1 for 40K. In order to ascertain the radiological safety of the population regarding maize consumption, the daily intakes of these radionuclides as well as the annual effective dose were estimated. The total effective dose obtained due to the ingestion of radionuclides via maize consumption is 15.39 μSv y-1, which is less than the international recommendations.

  9. Is the basal area of maize internodes involved in borer resistance?

    OpenAIRE

    Malvar Rosa; Revilla Pedro; Butrón Ana; Santiago Rogelio

    2011-01-01

    Abstract Background To elucidate the role of the length of the internode basal ring (LIBR) in resistance to the Mediterranean corn borer (MCB), we carried out a divergent selection program to modify the LIBR using two maize synthetic varieties (EPS20 and EPS21), each with a different genetic background. We investigated the biochemical mechanisms underlying the relationship between the LIBR and borer resistance. Selection to lengthen or shorten the LIBR was achieved for each synthetic variety....

  10. Separating heat stress from moisture stress: analyzing yield response to high temperature in irrigated maize

    Science.gov (United States)

    Carter, Elizabeth K.; Melkonian, Jeff; Riha, Susan J.; Shaw, Stephen B.

    2016-09-01

    Several recent studies have indicated that high air temperatures are limiting maize (Zea mays L.) yields in the US Corn Belt and project significant yield losses with expected increases in growing season temperatures. Further work has suggested that high air temperatures are indicative of high evaporative demand, and that decreases in maize yields which correlate to high temperatures and vapor pressure deficits (VPD) likely reflect underlying soil moisture limitations. It remains unclear whether direct high temperature impacts on yields, independent of moisture stress, can be observed under current temperature regimes. Given that projected high temperature and moisture may not co-vary the same way as they have historically, quantitative analyzes of direct temperature impacts are critical for accurate yield projections and targeted mitigation strategies under shifting temperature regimes. To evaluate yield response to above optimum temperatures independent of soil moisture stress, we analyzed climate impacts on irrigated maize yields obtained from the National Corn Growers Association (NCGA) corn yield contests for Nebraska, Kansas and Missouri. In irrigated maize, we found no evidence of a direct negative impact on yield by daytime air temperature, calculated canopy temperature, or VPD when analyzed seasonally. Solar radiation was the primary yield-limiting climate variable. Our analyses suggested that elevated night temperature impacted yield by increasing rates of phenological development. High temperatures during grain-fill significantly interacted with yields, but this effect was often beneficial and included evidence of acquired thermo-tolerance. Furthermore, genetics and management—information uniquely available in the NCGA contest data—explained more yield variability than climate, and significantly modified crop response to climate. Thermo-acclimation, improved genetics and changes to management practices have the potential to partially or completely

  11. Identity, regulation, and activity of inducible diterpenoid phytoalexins in maize.

    Science.gov (United States)

    Schmelz, Eric A; Kaplan, Fatma; Huffaker, Alisa; Dafoe, Nicole J; Vaughan, Martha M; Ni, Xinzhi; Rocca, James R; Alborn, Hans T; Teal, Peter E

    2011-03-29

    Phytoalexins constitute a broad category of pathogen- and insect-inducible biochemicals that locally protect plant tissues. Because of their agronomic significance, maize and rice have been extensively investigated for their terpenoid-based defenses, which include insect-inducible monoterpene and sesquiterpene volatiles. Rice also produces a complex array of pathogen-inducible diterpenoid phytoalexins. Despite the demonstration of fungal-induced ent-kaur-15-ene production in maize over 30 y ago, the identity of functionally analogous maize diterpenoid phytoalexins has remained elusive. In response to stem attack by the European corn borer (Ostrinia nubilalis) and fungi, we observed the induced accumulation of six ent-kaurane-related diterpenoids, collectively termed kauralexins. Isolation and identification of the predominant Rhizopus microsporus-induced metabolites revealed ent-kaur-19-al-17-oic acid and the unique analog ent-kaur-15-en-19-al-17-oic acid, assigned as kauralexins A3 and B3, respectively. Encoding an ent-copalyl diphosphate synthase, fungal-induced An2 transcript accumulation precedes highly localized kauralexin production, which can eventually exceed 100 μg · g(-1) fresh weight. Pharmacological applications of jasmonic acid and ethylene also synergize the induced accumulation of kauralexins. Occurring at elevated levels in the scutella of all inbred lines examined, kauralexins appear ubiquitous in maize. At concentrations as low as 10 μg · mL(-1), kauralexin B3 significantly inhibited the growth of the opportunistic necrotroph R. microsporus and the causal agent of anthracnose stalk rot, Colletotrichum graminicola. Kauralexins also exhibited significant O. nubilalis antifeedant activity. Our work establishes the presence of diterpenoid defenses in maize and enables a more detailed analysis of their biosynthetic pathways, regulation, and crop defense function.

  12. Planting date impacts on soil water management, plant growth, and weeds in cover-crop-based no-till corn production

    Science.gov (United States)

    Low input and organic farmers are increasingly utilizing cover crop mulches in maize production. Many farmers are delaying planting corn into these high residue environments to allow greater growth of the cover crop to maximize nitrogen fixation and improve mechanical termination with roller crimpe...

  13. Conserved and distinct functions of the "stunted" (StuA)-Homolog Ust1 during cell differentiation in the corn smut fungus Ustilago maydis

    Science.gov (United States)

    Ustilago maydis, causal agent of corn smut, is a model for obligate fungal plant pathogens because, although it can proliferate saprobically in its yeast form, the infectious filamentous form is absolutely dependent on the host to complete its life cycle. Maize responds to U. maydis colonization by...

  14. Characterisation of cysteine proteinases responsible for digestive proteolysis in guts of larval Western corn rootworm (Diabrotica virgifera) by expression in the yeast Pichia pastoris

    NARCIS (Netherlands)

    Bown, D.P.; Wilkinson, H.S.; Jongsma, M.A.; Gatehouse, J.A.

    2004-01-01

    Cysteine proteinases are the major class of enzymes responsible for digestive proteolysis in western corn rootworm (Diabrotica virgifera), a serious pest of maize. A larval gut extract hydrolysed typical cathepsin substrates, such as Z-phe-arg-AMC and Z-arg-arg-AMC, and hydrolysis was inhibited by Z

  15. Evaluation of corn germplasm lines for multiple ear-colonizing insect and disease resistance.

    Science.gov (United States)

    Ni, Xinzhi; Xu, Wenwei; Blanco, Michael H; Wilson, Jeffrey P

    2012-08-01

    Ear-colonizing insects and diseases that reduce yield and impose health threats by mycotoxin contaminations in the grain, are critical impediments for corn (Zea mays L.) production in the southern United States. Ten germplasm lines from the Germplasm Enhancement of Maize (GEM) Program in Ames, IA, and Raleigh, NC, and 10 lines (derived from GEM germplasm) from the Texas Agricultural Experiment Station in Lubbock, TX, were examined in 2007 and 2008 with local resistant and susceptible controls. Four types of insect damage and smut disease (Ustilago maydis) infection, as well as gene X environment (G X E) interaction, was assessed on corn ears under field conditions. Insect damage on corn ears was further separated as cob and kernel damage. Cob penetration rating was used to assess corn earworm [Helicoverpa zea (Boddie)] and fall armyworm [Spodoptera frugiperda (J.E. Smith)] feeding on corn cobs, whereas kernel damage was assessed using three parameters: 1) percentage of kernels discolored by stink bugs (i.e., brown stink bug [Euschistus serous (Say)], southern green stink bug [Nezara viridula (L.)], and green stink bug [Chinavia (Acrosternum) hilare (Say)]; 2) percentage of maize weevil (Sitophilus zeamais Motschulsky)-damaged kernels; and 3) percentage of kernels damaged by sap beetle (Carpophilus spp.), "chocolate milkworm" (Moodna spp.), and pink scavenger caterpillar [Pyroderces (Anatrachyntis) rileyi (Walsingham)]. The smut infection rates on ears, tassels, and nodes also were assessed. Ear protection traits (i.e., husk tightness and extension) in relation to insect damage and smut infection also were examined. Significant differences in insect damage, smut infection, and husk protection traits were detected among the germplasm lines. Three of the 20 germplasm lines were identified as being multiple insect and smut resistant. Of the three lines, entries 5 and 7 were derived from DKXL370, which was developed using corn germplasm from Brazil, whereas entry 14 was

  16. Glycerol inclusion levels in corn and sunflower silages

    Directory of Open Access Journals (Sweden)

    Adriana de Souza Martins

    2014-10-01

    Full Text Available Due to the seasonal cycle of forage, the use of silage to feed animals provides nutrients throughout the year. However, its quality can be improved with the inclusion of additives and other products. Glycerol is a rich source of energy and present a high efficiency of utilization by animals. The purpose of this work was to evaluate the effect of glycerol inclusion on the chemical and fermentation characteristics of corn and sunflower silages. Two silage sources (maize and sunflower were used and four levels of glycerol inclusion (0, 15, 30 and 45% based on dry matter were carried out. The experimental design was completely randomized in a 2 x 4 factorial arrangement with five replications. The pH values and chemical composition of corn and sunflower silages were determined. In both silages there was increment of dry matter, non-fiber carbohydrates and total digestible nutrients (TDN added to a reduction of crude protein, neutral detergent fiber and acid detergent fiber due to the glycerol inclusion. The corn silage required 45% glycerol to achieve the TDN level of the sunflower silage. The glycerol addition contributed to the increase in the nutritional value, offsetting loss of quality in the ensiling process.

  17. Biotechnology in maize breeding

    Directory of Open Access Journals (Sweden)

    Mladenović-Drinić Snežana

    2004-01-01

    Full Text Available Maize is one of the most important economic crops and the best studied and most tractable genetic system among monocots. The development of biotechnology has led to a great increase in our knowledge of maize genetics and understanding of the structure and behaviour of maize genomes. Conventional breeding practices can now be complemented by a number of new and powerful techniques. Some of these often referred to as molecular methods, enable scientists to see the layout of the entire genome of any organism and to select plants with preferred characteristics by "reading" at the molecular level, saving precious time and resources. DNA markers have provided valuable tools in various analyses ranging from phylogenetic analysis to the positional cloning of genes. Application of molecular markers for genetic studies of maize include: assessment of genetic variability and characterization of germ plasm, identification and fingerprinting of genotypes, estimation of genetic distance, detection of monogamic and quantitative trait loci, marker assisted selection, identification of sequence of useful candidate genes, etc. The development of high-density molecular maps which has been facilitated by PCR-based markers, have made the mapping and tagging of almost any trait possible and serve as bases for marker assisted selection. Sequencing of maize genomes would help to elucidate gene function, gene regulation and their expression. Modern biotechnology also includes an array of tools for introducing or deieting a particular gene or genes to produce plants with novel traits. Development of informatics and biotechnology are resulted in bioinformatic as well as in expansion of microarrey technique. Modern biotechnologies could complement and improve the efficiency of traditional selection and breeding techniques to enhance agricultural productivity.

  18. Genetic analysis of maturity and flowering characteristics in maize (Zea mays L.)

    Institute of Scientific and Technical Information of China (English)

    Hassan Sher; Muhammad Iqbal; Kiramat Khan; Muhammad yasir; Hameed-ur-Rahman

    2012-01-01

    Objective: To elucidate the pattern of inheritance and determine the relative magnitude of various genetic effects for maturity and flowering attributes in subtropical maize. Methods:Four white grain maize inbred lines from flint group of corn, two with late maturity and two with early maturity, were used. These contrasting inbred lines were crossed to form four crosses. Six generations (P1, P2, F1, F2, BC1, and BC2) were developed for each individual cross. These were evaluated in triplicate trial for two consecutive years. Results: Both dominance gene action and epistatic interaction played major role in governing inheritance of days to pollen shedding, 50% silking, anthesis silking interval and maturity. Conclusions: Preponderance of dominance gene action for these traits indicated their usefulness in hybrid programs of subtropical maize.

  19. Short communication: QTL mapping for ear tip-barrenness in maize

    Energy Technology Data Exchange (ETDEWEB)

    Ding, J.; Ma, J.; Chen, J.; Ai, T.; Li, Z.; Tian, Z.; Wu, S.; Chen, W.; Wu, J.

    2016-11-01

    Barren tip on corn ear is an important agronomic trait in maize, which is highly associated with grain yield. Understanding the genetic basis of tip-barrenness may help to reduce the ear tip-barrenness in breeding programs. In this study, ear tip-barrenness was evaluated in two environments in a F2:3 population, and it showed significant genotypic variation for ear tip-barrenness in both environments. Using mixed-model composite interval mapping method, three additive effects quantitative trait loci (QTL) for ear tip-barrenness were mapped on chromosomes 2, 3 and 6, respectively. They explained 16.6% of the phenotypic variation, and no significant QTL × Environment interactions and digenic interactions were detected. The results indicated that additive effect was the main genetic basis for ear tip-barrenness in maize. This is the first report of QTL mapped for ear tip-barrenness in maize. (Author)

  20. High yield cultivation technology of summer corn%夏玉米优质高产栽培技术

    Institute of Scientific and Technical Information of China (English)

    郭雪莲

    2011-01-01

    依据夏玉米各生育阶段的主攻目标,通过对夏玉米各生育阶段生长发育的调查、分析,总结出适合夏玉米高产稳产的管理及栽培技术。%On the basis of summer corn in different growth stages of the main attack target, through to summer maize at different growth stages of growth and development survey, analysis, summed up for summer maize high yield management and cultivation techniques.

  1. Global warming presents new challenges for maize pest management

    Science.gov (United States)

    Diffenbaugh, Noah S.; Krupke, Christian H.; White, Michael A.; Alexander, Corinne E.

    2008-10-01

    It has been conjectured that global warming will increase the prevalence of insect pests in many agro-ecosystems. In this paper, we quantitatively assess four of the key pests of maize, one of the most important systems in North American grain production. Using empirically generated estimates of pest overwintering thresholds and degree-day requirements, along with climate change projections from a high-resolution climate model, we project potential future ranges for each of these pests in the United States. Our analysis suggests the possibility of increased winter survival and greater degree-day accumulations for each of the pests surveyed. We find that relaxed cold limitation could expand the range of all four pest taxa, including a substantial range expansion in the case of corn earworm (H. zea), a migratory, cold-intolerant pest. Because the corn earworm is a cosmopolitan pest that has shown resistance to insecticides, our results suggest that this expansion could also threaten other crops, including those in high-value areas of the western United States. Because managing significant additional pressure from this suite of established pests would require additional pest management inputs, the projected decreases in cold limitation and increases in heat accumulation have the potential to significantly alter the pest management landscape for North American maize production. Further, these range expansions could have substantial economic impacts through increased seed and insecticide costs, decreased yields, and the downstream effects of changes in crop yield variability.

  2. Maize kernel size and texture: production parameters, quality of eggs of the laying hens and electricity intake

    Directory of Open Access Journals (Sweden)

    Javer Alves Vieira Filho

    2015-08-01

    Full Text Available The influence of maize corn size and texture on the performance parameters of laying hens and power consumption required for grinding maize corn were evaluated. The experiment was carried out on 384 Isa Brown hens, 36 weeks old, penned in a conventional aviary with 562.5 cm2 bird-1 stocking rate. The treatments were distributed in a completely randomized 2 x 3 factorial design (maize textures: flint and dent; and milling degree: fine, medium and coarse with eight replicates of eight birds per plot. Data were evaluated with SISVAR and means were compared by Tukey’s test at 5% probability. Difference was reported for the variable texture and flint increased the variables feed intake and egg weight. Significant difference in the characteristics of egg quality occurred only for the colorof the yolk. Larger corn sizes consumed less electricity during grinding. The maize flint cultivar had a lower 31.7% power consumption when compared to that of the dent cultivar.

  3. THE INFLUENCE OF ABIOTIC FACTORS ON THE PRESENCE OF EUROPEAN CORN BORER (Ostrinia nubilalis Hubner

    Directory of Open Access Journals (Sweden)

    Ankica Sarajlić

    2015-12-01

    Full Text Available Field experiments with natural population of European corn borer (ECB were conducted in three vegetation seasons (2012-2014 at Agricultural Institute in Osijek. The experiment was set up in a randomized block design as split-split plot method, with three repetitions. This plot has been constantly maize - soybean rotation for already 15 years. It was a 3x3x4 factorial experiment with three irrigation levels (A1- non-irrigated (only natural precipitation, A2-from 60% to 80% field water capacity - FWC and A3-from 80% to100% FWC, three nitrogen fertilizer levels (B1-0, B2-100 and B3-200 kg N/ha and four different genotypes (C1-0SSK 596; C2-0SSK 617; C3-0SSK 602 and C4-0SSK 552.The aim of this study was to determine the effect of different levels of irrigation, nitrogen fertilization and genotypes on occurrence and damage of maize plants by the European corn borer larvae and relation between leaf feeding larvae with nitrogen and silicon concentration as well as C/N ratio. At the end of each growing season, ten maize plants from each variant were cut. Ear weight for each specific plant (g, tunnel length (cm, number of larvae in stalk, number of larvae in the ear shank, ear shank damage (cm and total number of larvae in maize plantwere determined. In silking stage (middle of July ten leaves (below the ear, from 10 maize plants were sampled on each variant. Nitrogen, carbon and silicon concentrations were determined in maize leaf (% and C/N ratio calculated. In 2014, a significantly lower ECB attack was determined taking into account lower temperatures and higher amount of precipitate compared to the previous years. Dominance of Z-type European corn borer on pheromone traps in the area of eastern Slavonia was confirmed. Increasing the level of soil water content, damage from larvae was reduced and increasing the level of nitrogen fertilization feeding activity was increased. We have confirmed different hybrid resistance in regards to damage from larvae

  4. Bt-maize event MON 88017 expressing Cry3Bb1 does not cause harm to non-target organisms.

    Science.gov (United States)

    Devos, Yann; De Schrijver, Adinda; De Clercq, Patrick; Kiss, József; Romeis, Jörg

    2012-12-01

    This review paper explores whether the cultivation of the genetically modified Bt-maize transformation event MON 88017, expressing the insecticidal Cry3Bb1 protein against corn rootworms (Coleoptera: Chrysomelidae), causes adverse effects to non-target organisms (NTOs) and the ecological and anthropocentric functions they provide. Available data do not reveal adverse effects of Cry3Bb1 on various NTOs that are representative of potentially exposed taxonomic and functional groups, confirming that the insecticidal activity of the Cry3Bb1 protein is limited to species belonging to the coleopteran family of Chrysomelidae. The potential risk to non-target chrysomelid larvae ingesting maize MON 88017 pollen deposited on host plants is minimal, as their abundance in maize fields and the likelihood of encountering harmful amounts of pollen in and around maize MON 88017 fields are low. Non-target adult chrysomelids, which may occasionally feed on maize MON 88017 plants, are not expected to be affected due to the low activity of the Cry3Bb1 protein on adults. Impacts on NTOs caused by potential unintended changes in maize MON 88017 are not expected to occur, as no differences in composition, phenotypic characteristics and plant-NTO interactions were observed between maize MON 88017 and its near-isogenic line.

  5. Establishing alfalfa in silage corn

    Science.gov (United States)

    According to recent agricultural statistics, alfalfa was planted on 0.44 million acres and harvested from 2.2 million acres and silage corn was planted and harvested from 1.0 million acres per year in Wisconsin. Because both crops are often grown in rotation, alfalfa could be interseeded at corn pla...

  6. Establishing alfalfa in corn silage

    Science.gov (United States)

    According to recent agricultural statistics, alfalfa was planted on 0.44 million acres and harvested from 2.2 million acres, and corn silage was planted and harvested from 1.0 million acres per year in Wisconsin. Because both crops are often grown in rotation, alfalfa could be interseeded at corn pl...

  7. Relevance of traditional integrated pest management (IPM) strategies for commercial corn producers in a transgenic agroecosystem: a bygone era?

    Science.gov (United States)

    Gray, Michael E

    2011-06-08

    The use of transgenic Bt maize hybrids continues to increase significantly across the Corn Belt of the United States. In 2009, 59% of all maize planted in Illinois was characterized as a "stacked" gene variety. This is a 40% increase since 2006. Stacked hybrids typically express one Cry protein for corn rootworm control and one Cry protein for control of several lepidopteran pests; they also feature herbicide tolerance (to either glyphosate or glufosinate). Slightly more than 50 years has passed since Vernon Stern and his University of California entomology colleagues published (1959) their seminal paper on the integrated control concept, laying the foundation for modern pest management (IPM) programs. To assess the relevance of traditional IPM concepts within a transgenic agroecosystem, commercial maize producers were surveyed at a series of meetings in 2009 and 2010 regarding their perceptions on their use of Bt hybrids and resistance management. Special attention was devoted to two insect pests of corn, the European corn borer and the western corn rootworm. A high percentage of producers who participated in these meetings planted Bt hybrids in 2008 and 2009, 97 and 96.7%, respectively. Refuge compliance in 2008 and 2009, as mandated by the U.S. Environmental Protection Agency (EPA), was 82 and 75.7%, respectively, for those producers surveyed. A large majority of producers (79 and 73.3% in 2009 and 2010, respectively) revealed that they would, or had, used a Bt hybrid for corn rootworm (Diabrotica virgifera virgifera LeConte) or European corn borer (Ostrinia nubilalis Hübner) control even when anticipated densities were low. Currently, the EPA is evaluating the long-term use of seed blends (Bt and non-Bt) as a resistance management strategy. In 2010, a large percentage of producers, 80.4%, indicated they would be willing to use this approach. The current lack of integration of management tactics for insect pests of maize in the U.S. Corn Belt, due primarily to

  8. Effect of Malting and Nixtamalization Processes on the Physicochemical Properties of Instant Extruded Corn Flour and Tortilla Quality.

    Science.gov (United States)

    Rodríguez-Martínez, Nicolás Alberto; Salazar-García, María Guadalupe; Ramírez-Wong, Benjamín; Islas-Rubio, Alma Rosa; Platt-Lucero, Luis Carlos; Morales-Rosas, Ignacio; Marquez-Melendez, Rubén; Martínez-Bustos, Fernando

    2015-09-01

    This research aimed to prepare instant flour from malted and raw (un-malted) corn flours nixtamalized by the extrusion process and evaluate the effect on the physicochemical properties of tortillas prepared using these flours. White maize was malted for 24 h, dried at 50 ± 1 °C, and ground. Subsequently, 0.3 % lime and 25 or 30 % water were added to ground malted or un-malted corn, and the mixture was refrigerated (4 °C) for 12 h. These samples were nixtamalized by an extrusion process in a single screw extruder at two temperature profiles within four heating zones, TP1 (60, 60, 70, and 80 °C) and TP2 (60, 70, 80, and 90 °C), to obtain corn flour. Water was added to the extruded corn flours to make a dough, or masa, and the masa was then molded and baked to obtain tortillas. The corn flours were characterized according to their ability to absorb water and viscosity profile (RVA). The firmness and rollability after 2 and 24 h of storage were determined, and a sensory evaluation was conducted. The malted corn flour extruded with a 25 % moisture content and TP2 temperature profile yielded tortillas with the best firmness and rollability. In conclusion, the changes during the malting of corn grain and the nixtamalization by the extrusion process improved the water absorption capacity of flours and textural properties of the tortilla and produced a product with acceptable sensory properties.

  9. Arbuscular mycorrhizal fungi and colonization stimulant in cotton and maize

    Directory of Open Access Journals (Sweden)

    Fabrício Henrique Moreira Salgado

    Full Text Available ABSTRACT: The objective of this study was to evaluate the effects of inoculation with different arbuscular mycorrhizal fungi native to Cerrado, associated with the application of colonization stimulant (7-hydroxy, 4’-methoxy-isoflavone at the initial growth of cotton and maize plants in a greenhouse. The experiment was carried out in a completely randomized design and 7x2 factorial scheme, with five species of arbuscular mycorrhizal fungi and combined inoculation (uniting all species in equal proportion and native fungi, in the presence and absence of colonization stimulant in maize and cotton crops. Shoots dry matter (SDM, roots dry matter (RDM, mycorrhizal colonization and accumulation of calcium, zinc and phosphorus in the SDM were evaluated at flowering. Inoculation of arbuscular mycorrhizal fungi and the use of colonization stimulant at low density of arbuscular mycorrhizal fungi propagules was beneficial to plant development. Inoculation with mycorrhizal fungi demonstrated different effects for the various parameters evaluated in cotton and maize, and the application of colonization stimulant promoted higher mycorrhizal colonization and initial growth in cotton, when associated with native AMF, and in corn, when associated with inoculation with Dentiscutata heterogama, Gigaspora margarita and Rhizophagus clarus.

  10. Role of Dehydrodiferulates in Maize Resistance to Pests and Diseases

    Directory of Open Access Journals (Sweden)

    Rosa A. Malvar

    2010-02-01

    Full Text Available Phenolic esters have attracted considerable interest due to the potential they offer for peroxidase catalysed cross-linking of cell wall polysaccharides. Particularly, feruloyl residues undergo radical coupling reactions that result in cross-linking (intra-/intermolecular between polysaccharides, between polysaccharides and lignin and, between polysaccharides and proteins. This review addresses for the first time different studies in which it is established that cross-linking by dehydrodiferulates contributes to maize’s defences to pests and diseases. Dehydrodiferulate cross-links are involved in maize defence mechanisms against insects such as the European, Mediterranean, and tropical corn borers and, storage pest as the maize weevil. In addition, cross-links are also discussed to be involved in genetic resistance of maize to fungus diseases as Gibberella ear and stalk rot. Resistance against insects and fungus attending dehydrodiferulates could go hand in hand. Quantitative trait loci mapping for these cell wall components could be a useful tool for enhancing resistance to pest and diseases in future breeding programs.

  11. Effect of insertion of Bt gene in corn and different fumonisin content on growth performance of weaned piglets

    Directory of Open Access Journals (Sweden)

    Filippo Rossi

    2011-04-01

    Full Text Available The objective of this study was to compare the effect of Bt corn and isogenic corn on the growth of weaned piglets. One hundred twenty-eight weaned piglets weighing 8.8 ±1.27 kg live weight were randomly assigned to 4 groups of 32 animals each (16 castrated males and 16 females. Bt corn (line MON810 and isogenic corn were produced at two farms located in the Lodi and Venezia provinces (northern Italy. Bt corn had the same chemical composition as the isogenic corn but a lower content of fumonisin B1 (FB1. The experimental period (35 days was divided into two phases: 0-14 d and 15-35 d. There was no significant difference in average daily gain (ADG among groups during the first feeding phase. Compared to animals fed isogenic corn, the piglets fed Bt maize gained more weight during the second feeding phase (Bt: 464.1 g/d, isogenic: 429.1 g/d; P < 0.05. Also, the ADG over the entire trial was higher in piglets fed Bt corn versus piglets fed isogenic corn (Bt: 396.4 g/d, isogenic: 374.1 g/d; P < 0.05. The ADG of the whole period decreased linearly (P<0.05 with respect to FB1 content of diet. Final weight was higher in piglets fed the diet containing Bt corn (Bt: 22.68 kg, isogenic: 21.83 kg; P < 0.05. No differences in feed intake and in the feed:gain ratio were observed, however a linear response between FB1 and feed:gain ratio in first 14 days of the experiment was detected.

  12. Effect of insertion of Bt gene in corn and different fumonisin content on growth performance of weaned piglets

    Directory of Open Access Journals (Sweden)

    Filippo Rossi

    2010-05-01

    Full Text Available The objective of this study was to compare the effect of Bt corn and isogenic corn on the growth of weaned piglets. One hundred and twenty-eight weaned piglets weighing 8.8±1.27 kg live weight were randomly assigned to 4 groups of 32 animals each (16 castrated males and 16 females. Bt corn (line MON810 and isogenic corn were produced at two farms located in the provinces of Lodi and Venice (northern Italy. The Bt corn had the same chemical composition as the isogenic corn but a lower content of fumonisin B1 (FB1. The experimental period (35 days was in 2 phases, 0-14 d and 15-35 d. There was no significant difference in average daily gain (ADG among groups during the first feeding phase. Compared to animals fed isogenic corn, the piglets fed Bt maize gained more weight during the second feeding phase (Bt: 464.1 g/d, isogenic: 429.1 g/d; P<0.05. Also, the ADG over the entire trial was higher in piglets fed Bt corn versus piglets fed isogenic corn (Bt: 396.4 g/d, isogenic: 374.1 g/d; P<0.05. The ADG of the whole period decreased linearly (P<0.05 with respect to the FB1 content of the diet. Final weight was higher in piglets fed the diet containing Bt corn (Bt: 22.68 kg, isogenic: 21.83 kg; P<0.05. No differences in feed intake and in the feed:gain ratio were observed, although a linear response between FB1 and feed:gain ratio in first 14 days of the experiment was detected.

  13. The Maize megagametophyte

    OpenAIRE

    Evans, M M S; U. Grossniklaus

    2008-01-01

    The life cycle of plants alternates between a diploid and a haploid generation. In flowering plants the haploid gametophytes are sexually dimorphic and produce the gametes, which fuse to produce the diploid sporophyte of the next generation. The megagametophyte of maize follows the Polygonum-type pattern of development:one of the four meiotic products, the functional megaspore, undergoes three free nuclear divisions to produce a polarized, eight-nucleate syncytium. Cellularization produces se...

  14. 21 CFR 184.1262 - Corn silk and corn silk extract.

    Science.gov (United States)

    2010-04-01

    ... filaments are extracted with dilute ethanol to produce corn silk extract. The extract may be concentrated at... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Corn silk and corn silk extract. 184.1262 Section... SAFE Listing of Specific Substances Affirmed as GRAS § 184.1262 Corn silk and corn silk extract....

  15. Screening for corn rootworm (Coleoptera: Chrysomelidae) resistance to transgenic Bt corn in North Dakota

    Science.gov (United States)

    Western (WCR), Diabrotica virgifera virgifera LeConte, and northern corn rootworms (NCR), D. barberi Smith & Lawrence, are major economic pests of corn in much of the U.S. Corn Belt. Western corn rootworm resistance to transgenic corn expressing Bt (Bacillus thuringiensis) endotoxins has been confi...

  16. 9 CFR 319.100 - Corned beef.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Corned beef. 319.100 Section 319.100... Corned beef. “Corned Beef” shall be prepared from beef briskets, navels, clods, middle ribs, rounds... A or Subchapter B. Canned product labeled “Corned Beef” shall be prepared so that the weight of...

  17. Effects of Pyramided Bt Corn and Blended Refuges on Western Corn Rootworm and Northern Corn Rootworm (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Keweshan, Ryan S; Head, Graham P; Gassmann, Aaron J

    2015-04-01

    The western corn rootworm, Diabrotica virgifera virgifera LeConte, and the northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae), are major pests of corn (Zea mays L). Several transgenic corn events producing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) kill corn rootworm larvae and reduce injury to corn roots. However, planting of Bt corn imposes selection on rootworm populations to evolve Bt resistance. The refuge strategy and pyramiding of multiple Bt toxins can delay resistance to Bt crops. In this study, we assessed the impact of four treatments--1) non-Bt corn, 2) Cry3Bb1 corn, 3) corn pyramided with Cry3Bb1 and Cry34/35Ab1, and 4) pyramided corn with a blended refuge--on survival, time of adult emergence, and size of western and northern corn rootworm. All treatments with Bt corn led to significant reductions in the number of adults that emerged per plot. However, at one location, we identified Cry3Bb1-resistant western corn rootworm. In some cases Bt treatments reduced size of adults and delayed time of adult emergence, with effects most pronounced for pyramided corn. For both species, the number of adults that emerged from pyramided corn with a blended refuge was significantly lower than expected, based solely on emergence from pure stands of pyramided corn and non-Bt corn. The results of this study indicate that pyramided corn with a blended refuge substantially reduces survival of both western and northern corn rootworm, and as such, should be a useful tool within the context of a broader integrated pest management strategy.

  18. Ethanol extraction of phytosterols from corn fiber

    Science.gov (United States)

    Abbas, Charles; Beery, Kyle E.; Binder, Thomas P.; Rammelsberg, Anne M.

    2010-11-16

    The present invention provides a process for extracting sterols from a high solids, thermochemically hydrolyzed corn fiber using ethanol as the extractant. The process includes obtaining a corn fiber slurry having a moisture content from about 20 weight percent to about 50 weight percent solids (high solids content), thermochemically processing the corn fiber slurry having high solids content of 20 to 50% to produce a hydrolyzed corn fiber slurry, dewatering the hydrolyzed corn fiber slurry to achieve a residual corn fiber having a moisture content from about 30 to 80 weight percent solids, washing the residual corn fiber, dewatering the washed, hydrolyzed corn fiber slurry to achieve a residual corn fiber having a moisture content from about 30 to 80 weight percent solids, and extracting the residual corn fiber with ethanol and separating at least one sterol.

  19. Maize variety and method of production

    Energy Technology Data Exchange (ETDEWEB)

    Pauly, Markus; Hake, Sarah; Kraemer, Florian J

    2014-05-27

    The disclosure relates to a maize plant, seed, variety, and hybrid. More specifically, the disclosure relates to a maize plant containing a Cal-1 allele, whose expression results in increased cell wall-derived glucan content in the maize plant. The disclosure also relates to crossing inbreds, varieties, and hybrids containing the Cal-1 allele to produce novel types and varieties of maize plants.

  20. Ubi1 intron-mediated enhancement of the expression of Bt cry1Ah gene in transgenic maize (Zea mays L.)

    Institute of Scientific and Technical Information of China (English)

    WANG YueBing; LANG Zhihong; ZHANG Jie; HE KangLai; SONG FuPing; HUANG DaFang

    2008-01-01

    The cry1Ah gone was one of novel insecticidal genes cloned from Bacillus thuringiensis isolate BT8. Two plant expression vectors containing cry1Ah gone were constructed. The first intron of maize ubiqutin1 gone was inserted between the maize Ubiquitin promoter and cry1Ah gone in one of the plant expressing vectors (pUUOAH). The two vectors were introduced into maize immature embryonic calli by microprojectile bombardment, and the reproductively plants were acquired. PCR and Southern blot analysis showed that foreign genes had been integrated into maize genome and inherited to the next generation stably. The ELISA assay to T1 and T2 generation plants showed that the expression of Cry1Ah protein in the construct containing the ubi1 intron (pUUOAH) was 20% higher than that of the intronless construct (pUOAH). Bioassay results showed that the transgenic maize harboring cry1Ah gone had high resistance to the Asian corn borers and the insecticidal activity of the transgenic maize containing the ubi1 intron was higher than that of the intronless construct. These results indicated that the maize ubil intron can enhance the expression of the Bt cry1Ah gone in transgenic maize efficiently

  1. Sorghum as an alternative of cultivation to maize; Sorghumhirse als Anbaualternative zum Mais

    Energy Technology Data Exchange (ETDEWEB)

    Jaekel, Kerstin; Theiss, Markus; Poetzschke, Karen [Saechsisches Landesamt fuer Umwelt, Landwirtschaft und Geologie (LfULG), Dresden (Germany)] [and others

    2013-10-01

    Due to their high dry matter yield potential Sorghum bicolor and Sorghum bicolor x sudanense are well fitted as feedstock for biogas production. Similar to maize, both species show a high efficiency in their use of water (C4-plants). However, Sorghum has a higher drought tolerance in comparison with maize but is more sensitive to low temperatures. Hence a cultivation of Sorghum is recommendable especially in dry and relatively warm regions, including recultivated areas and even on loess soil, provided that the required temperatures are given. Due to the fact that Sorghum is not affected by the corn root worm, it also could gain relevance in regions were the cultivation of maize is restricted. Furthermore, Sorghum is usable as a catch crop as well as a main crop because of its variable sowing time. Catch crop cultivation, however, yields a significantly lower amount of dry matter and -quality which is a result of its shorter vegetation period. Owing to its higher crude fiber concentration Sorghum achieves a lower theoretically attainable specific methane yield (Weissbach) than maize. Thus only on rare occasions Sorghum does achieve methane yields per hectare that are comparable to maize. Eventually, the competitiveness of Sorghum greatly depends on provision of enhanced cultivars achieved through genetic improvement. (orig.)

  2. [Occurrence of Fusarium strains and their mycotoxins in corn silage. 7. Formation of deoxynivalenol (DON) in a silage corn plot artificially inoculated with Fusarium culmorum and the effect of silaging on the stability of the DON formed].

    Science.gov (United States)

    Lepom, P; Knabe, O; Baath, H

    1990-10-01

    The formation of deoxynivalenol in a maize plot inoculated with Fusarium culmorum was studied over a growing season. Already three weeks after inoculation 4.9 mg/kg of DON were measured in the infected ears. The toxin concentration increased continuously up to harvest after eight weeks to a value of 261 mg/kg. Ensilage experiments in laboratory scale silos have shown that the DON content of naturally contaminated corn-cob-mix was not reduced during the ensilage process. It was concluded that infection of maize plants by toxin-producing Fusarium species followed by DON production in the field seems to be the most probable way of contamination of maize silage with this mycotoxin.

  3. FERMENTATION PROCESS CHARACTERISTICS OF DIFFERENT MAIZE SILAGE HYBRIDS

    Directory of Open Access Journals (Sweden)

    Daniel Bíro

    2009-03-01

    Full Text Available The aim of this study was to detect the fermentation process differences in different hybrid maize silage. We conserved in laboratory conditions hybrids of whole maize plants with different length of the vegetative period (FAO number. Maize hybrids for silage were harvested in the vegetation stage of the milk-wax maturity of corn and the content of dry matter was from 377.7 to 422.8 g.kg-1. The highest content of dry matter was typical for silages made from the hybrids with FAO number 310 (400.0 g.kg-1 and FAO 300a (400.4 g.kg-1. The content of desirable lactic acid ranged from 23.7 g.kg-1 of dry matter (FAO 350 to 58.9 g.kg-1 of dry matter (FAO 420. We detected the occurrence of undesirable butyric acid in silages from hybrids FAO 250, 300b, 310 and 380. The highest content of total alcohols we found in silages made from hybrid with FAO number 240 (25.2 g.kg-1 of dry matter. Ammonia contents were in tested silages from 0.153 (FAO 270 to 0.223 g.kg-1 of dry matter (FAO 240. The lowest value of silage titration acidity we analyzed in silage made from hybrid FAO 420 (3.66. We observed in maize silages with different length of plant maturity tested in the experiment differences in content of lactic acid, total alcohols, titration acidity, pH and content of fermentation products.

  4. 玉米根茬与地膜分离技术研究%Research on Maize Stubble and Plastic Film Separation

    Institute of Scientific and Technical Information of China (English)

    张丽; 张佳喜; 刘旋峰; 蒋永新; 张海春; 喻晨

    2015-01-01

    Corn is one of our important food crops, it contains rich nutrition.After maize harvest, and corn stubble and mulch need to be classified.Technology of corn plastic recycling was studied from the perspective of maize root crop and membrane separation in this article, aimed to improve the efficiency of corn plastic recycling.This article mainly divided into five parts: the first part, introduction, mainly introduced the technology of maize root crop and membrane separation at home and abroad.The second part, analyzed the necessity of maize root crop and membrane separation.The third part, the corn plastic recycling technology was introduced.The fourth part, some related suggestions on corn plastic recycling were put forward from the environmental point, the future development of plastic recycling was put forward.The fifth part, conclusion.%玉米是我国的主要粮食作物之一,内含丰富的营养,玉米丰收之后需要将玉米根茬和地膜进行分离。为此,从玉米根茬与地膜分离的技术角度出发,研究玉米地膜回收技术,旨在有效提高玉米地膜的回收效率。本研究主要介绍了国内外关于玉米根茬与地膜分离的技术,分析了玉米根茬与地膜分离的必要性,介绍了玉米地膜回收技术,并从环保角度提出了玉米地膜回收的相关建议及未来地膜回收的发展趋势。

  5. The Comparison of Sugar Components in the Developing Grains of Sweet Corn and Normal Corn

    Institute of Scientific and Technical Information of China (English)

    LIU Peng; HU Chang-hao; DONG Shu-ting; WANG Kong-jun; ZHANG Ji-wang

    2003-01-01

    The sugar components and their dynamic variation in the developing grains of sweet corn(Zeamays L. seccharata Sturt)and normal corn (Zea mays L. indentata Sturt) were compared. There are WSP(water-soluble polysaccharides), sucrose, fructose, glucose, mannitol and sorbitol in both sweet corn and nor-mal corn, but no maltose. Two components with different degrees of polymerization (D. P. N) were detected inthe sweet corn; only one of them was detected in the normal corn 20 days after pollination. With the develop-ment of grains, the total soluble sugar content(TSS)in sweet corn increased, but in normal corn it decreased.The dynamic variation of WSP, sucrose, glucose, fructose, mannitol and sorbitol in sweet and normal corngrains are different. The contents of sugar components in the sweet corn grains are higher than that in the nor-mal corn. Sweet corn accumulates less starch than normal corn.

  6. Hydroxycinnamate Synthesis and Association with Mediterranean Corn Borer Resistance.

    Science.gov (United States)

    Santiago, Rogelio; Malvar, Rosa Ana; Barros-Rios, Jaime; Samayoa, Luis Fernando; Butrón, Ana

    2016-01-27

    Previous results suggest a relationship between maize hydroxycinnamate concentration in the pith tissues and resistance to stem tunneling by Mediterranean corn borer (MCB, Sesamia nonagrioides Lef.) larvae. This study performs a more precise experiment, mapping an F2 derived from the cross between two inbreds with contrasting levels for hydroxycinnamates EP125 × PB130. We aimed to co-localize genomic regions involved in hydroxycinnamate synthesis and resistance to MCB and to highlight the particular route for each hydroxycinnamate component in relation to the better known phenylpropanoid pathway. Seven quantitative trait loci (QTLs) for p-coumarate, two QTLs for ferulate, and seven QTLs for total diferulates explained 81.7, 26.9, and 57.8% of the genotypic variance, respectively. In relation to borer resistance, alleles for increased hydroxycinnamate content (affecting one or more hydroxycinnamate compounds) could be associated with favorable effects on stem resistance to MCB, particularly the putative role of p-coumarate in borer resistance.

  7. CAROTENOID RETENTION IN MINIMALLY PROCESSED BIOFORTIFIED GREEN CORN STORED UNDER RETAIL MARKETING CONDITIONS

    Directory of Open Access Journals (Sweden)

    Natália Alves Barbosa

    2015-08-01

    Full Text Available Storing processed food products can cause alterations in their chemical compositions. Thus, the objective of this study was to evaluate carotenoid retention in the kernels of minimally processed normal and vitamin A precursor (proVA-biofortified green corn ears that were packaged in polystyrene trays covered with commercial film or in multilayered polynylon packaging material and were stored. Throughout the storage period, the carotenoids were extracted from the corn kernels using organic solvents and were quantified using HPLC. A completely factorial design including three factors (cultivar, packaging and storage period was applied for analysis. The green kernels of maize cultivars BRS1030 and BRS4104 exhibited similar carotenoid profiles, with zeaxanthin being the main carotenoid. Higher concentrations of the carotenoids lutein, β-cryptoxanthin, and β-carotene, the total carotenoids and the total vitamin A precursor carotenoids were detected in the green kernels of the biofortified BRS4104 maize. The packaging method did not affect carotenoid retention in the kernels of minimally processed green corn ears during the storage period.

  8. Evaluation of Forage Yield and Important Agronomic Indices of Corn Affected by Intercropping Systems with Peanut and Nitrogen Rates

    Directory of Open Access Journals (Sweden)

    M Nabati nasaz

    2016-07-01

    Full Text Available Introduction Multiple cropping such as intercropping plays an important role in agriculture because of maximizing beneficial interactions. Intercropping of legumes and cereals is an old practice in tropical agriculture that dates back to ancient civilization. Maize-legume intercrops could substantially increase forage quantity and quality and decrease requirement for protein supplements (Ahmad et al., 2008. Intercropping of cereals and legumes is important for development of sustainable food production systems. This may be due to some of the potential benefits in intercropping systems such as high productivity and profitability, improvement of soil fertility through the additional supply of N by fixation and excretion from the component legume, efficient use of resources, reducing damage caused by pests, diseases and weeds and improvement of forage quality (Ahmad et al., 2008; Fernandez-Aparicio et al., 2007; Lithourgidis et al., 2006. The main advantage of intercropping is more efficient utilization of the available resources and the increased productivity compared with each sole crop of the mixture. Therefore, this experiment was conducted to evaluate agronomic characteristics of corn and Land equivalent ratio (LER under intercropping with peanut and different rates of nitrogen. Materials and methods In order to evaluate the forage yield and important agronomic indices of corn (Zea mays L. affected by intercropping systems with peanut and different nitrogen rates, this experiment was performed in the experimental field of agricultural and natural resource research center of Guilan province, Rasht, Iran, during 2013-14 cropping season as a split plot arrangement in randomized complete block design with three replications. Nitrogen rates, including of zero, 100, 200 and 300 kg per hectare as main plot and sole cropping of corn and peanut (Arachis hypogaea L., intercropping systems including of intercropping corn and peanut at ratio of 1:1, 2

  9. Development of Corn Stover Biofuel: Impacts on Corn and Soybean Markets and Land Rotation

    OpenAIRE

    Taheripour, Farzad; Tyner, Wallace E.; Fiegel, Julie

    2013-01-01

    This paper first develops a partial equilibrium (PE) model to examine impacts of converting corn stover to biofuel on markets for corn and soybeans at the national market level. The PE model links gasoline, corn ethanol, dried distiller grains, corn, soybeans, and soybean meal markets in the presence and absence of a viable market for corn stover. The model also includes a technology which converts corn stover to bio-gasoline (a drop-in biofuel). The model evaluates profitability of the ethan...

  10. Corn prices and alcohol production

    Energy Technology Data Exchange (ETDEWEB)

    Wangsness, W.

    1979-09-01

    Corn has attracted the most attention as a feedstock for alcohol production. The economics are computed on the basis of fixed costs for labor, taxes, depreciation, heat, and enzymes. Changes in feedstock prices are shown to determine whether corn is used for energy or protein as cattle feed. Comparisons of gasoline refined from imported oil and gasohol are made for a range of prices per bushel and oil prices per barrel. (DCK)

  11. Antagonistic and Biocontrol Potential of Trichoderma asperellum ZJSX5003 Against the Maize Stalk Rot Pathogen Fusarium graminearum.

    Science.gov (United States)

    Li, Yaqian; Sun, Ruiyan; Yu, Jia; Saravanakumar, Kandasamy; Chen, Jie

    2016-09-01

    The efficacy of seven strains of Trichoderma asperellum collected from the fields in Southern China was assessed against Fusarium graminearum (FG) the causal agent of corn stalk rot of maize were in vitro for their antagonistic properties followed by statistical model of principal compound analysis to identify the beneficial antagonist T. asperellum strain. The key factors of antagonist activity were attributed to a total of 13 factors including cell wall degrading enzymes (chitnase, protease and β-glucanases), secondary metabolites and peptaibols and these were analyzed from eight strains of Trichoderma. A linear regression model demonstrated that interaction of enzymes and secondary metabolites of T. asperellum strain ZJSX5003 enhanced the antagonist activity against FG. Further, this strain displayed a disease reduction of 71 % in maize plants inoculated with FG compared to negative control. Pointing out that the T. asperellum strain ZJSX5003 is a potential source for the development of a biocontrol agent against corn stalk rot.

  12. MaizeGDB: The Maize Genetics and Genomics Database.

    Science.gov (United States)

    Harper, Lisa; Gardiner, Jack; Andorf, Carson; Lawrence, Carolyn J

    2016-01-01

    MaizeGDB is the community database for biological information about the crop plant Zea mays. Genomic, genetic, sequence, gene product, functional characterization, literature reference, and person/organization contact information are among the datatypes stored at MaizeGDB. At the project's website ( http://www.maizegdb.org ) are custom interfaces enabling researchers to browse data and to seek out specific information matching explicit search criteria. In addition, pre-compiled reports are made available for particular types of data and bulletin boards are provided to facilitate communication and coordination among members of the community of maize geneticists.

  13. Susceptibility and aversion of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Cry1F Bt maize and considerations for insect resistance management.

    Science.gov (United States)

    Binning, Rachel R; Coats, Joel; Kong, Xiaoxiao; Hellmich, Richard L

    2014-02-01

    Bacillus thuringiensis (Bt) maize was developed primarily for North American pests such as European corn borer (Ostrinia nubilalis (Hübner)). However, most Bt maize products are also cultivated outside of North America, where the primary pests may be different and may have lower susceptibility to Bt toxins. Fall armyworm (Spodoptera frugiperda JE Smith) is an important pest and primary target of Bt maize in Central and South America. S. frugiperda susceptibility to Cry1F (expressed in event TC1507) is an example of a pest-by-toxin interaction that does not meet the high-dose definition. In this study, the behavioral and toxic response of S. frugiperda to Cry1F maize was investigated by measuring the percentage of time naive third instars spent feeding during a 3-min exposure. S. frugiperda also were exposed as third instars to Cry1F maize for 14 d to measure weight gain and survival. S. frugiperda demonstrated an initial, postingestive aversive response to Cry1F maize, and few larvae survived the 14 d exposure. The role of susceptibility and avoidance are discussed in the context of global IRM refuge strategy development for Bt products.

  14. Systemic virus-induced gene silencing allows functional characterization of maize genes during biotrophic interaction with Ustilago maydis.

    Science.gov (United States)

    van der Linde, Karina; Kastner, Christine; Kumlehn, Jochen; Kahmann, Regine; Doehlemann, Gunther

    2011-01-01

    Infection of maize (Zea mays) plants with the corn smut fungus Ustilago maydis leads to the formation of large tumors on the stem, leaves and inflorescences. In this biotrophic interaction, plant defense responses are actively suppressed by the pathogen, and previous transcriptome analyses of infected maize plants showed massive and stage-specific changes in host gene expression during disease progression. To identify maize genes that are functionally involved in the interaction with U. maydis, we adapted a virus-induced gene silencing (VIGS) system based on the brome mosaic virus (BMV) for maize. Conditions were established that allowed successful U. maydis infection of BMV-preinfected maize plants. This set-up enabled quantification of VIGS and its impact on U. maydis infection using a quantitative real-time PCR (qRT-PCR)-based readout. In proof-of-principle experiments, an U. maydis-induced terpene synthase was shown to negatively regulate disease development while a protein involved in cell death inhibition was required for full virulence of U. maydis. The results suggest that this system is a versatile tool for the rapid identification of maize genes that determine compatibility with U. maydis.

  15. Antagonistic and Biocontrol Potential of Trichoderma asperellum ZJSX5003 Against the Maize Stalk Rot Pathogen Fusarium graminearum

    OpenAIRE

    Li, Yaqian; Sun, Ruiyan; Yu, Jia; Saravanakumar, Kandasamy; Chen, Jie

    2016-01-01

    The efficacy of seven strains of Trichodermaasperellum collected from the fields in Southern China was assessed against Fusarium graminearum (FG) the causal agent of corn stalk rot of maize were in vitro for their antagonistic properties followed by statistical model of principal compound analysis to identify the beneficial antagonist T.asperellum strain. The key factors of antagonist activity were attributed to a total of 13 factors including cell wall degrading enzymes (chitnase, protease a...

  16. Nitrate leaching from Silage Maize

    OpenAIRE

    Hansen, Elly Møller; Eriksen, Jørgen

    2009-01-01

    During the last 20 years the area with maize in Denmark has increased dramatically and reached 163,000 ha in 2008. Silage maize is easy to grow, is a suitable fodder for cows and goes well with grass-clover in the diet. This means that silage maize is often found in crop rotations with grass-clover on sandy soils in western Denmark. The ploughing in of grass-clover fields poses a serious risk of increased nitrate leaching on a coarse sandy soil, even when carried out in spring. With increased...

  17. The Impact of Bio-Ethanol Conversion and Global Climate Change on Corn Economic Performanve of Indonesia

    Directory of Open Access Journals (Sweden)

    Yudi Ferrianta

    2012-09-01

    Full Text Available Many studies conclude that the rise in global food pricesdue to higher demand from the development of biofuels,climate anomalies, and increased of oil prices. Not onlythe food commodity index rose more than 60 percent, nonfoodcommodity price index also rose over 60 percent andcrude oil price index has increased even further above 60percent. The purpose of this study is to analyze the impact ofbio-ethanol conversion and global climate change on corneconomic performance of Indonesia. The results showed thatthe food crisis caused by climate anomalies lead the worldcorn prices rose 50 percent, impact on Indonesia corn importsfell by 11.86 percent. And the other hand, the energy crisisthat caused the corn used as feedstock for ethanol that causedU.S. corn exports only 20 percent of their products have animpact on Indonesia on maize imports fell 32.4 percent.

  18. Resistance of maize varieties to the maize weevil Sitophilus zeamais

    African Journals Online (AJOL)

    PRECIOUS

    2009-11-02

    Nov 2, 2009 ... as an increasingly important problem in Africa (Markham et al., 1994). Cheap and ... Removal of parent weevils and placement on a fresh seed medium ..... stored maize. An M.Sc. Thesis presented to the School of Graduate.

  19. Development of insect resistant maize plants expressing a chitinase gene from the cotton leaf worm, Spodoptera littoralis.

    Science.gov (United States)

    Osman, Gamal H; Assem, Shireen K; Alreedy, Rasha M; El-Ghareeb, Doaa K; Basry, Mahmoud A; Rastogi, Anshu; Kalaji, Hazem M

    2015-12-14

    Due to the importance of chitinolytic enzymes for insect, nematode and fungal growth, they are receiving attention concerning their development as biopesticides or chemical defense proteins in transgenic plants and as microbial biocontrol agents. Targeting chitin associated with the extracellular matrices or cell wall by insect chitinases may be an effective approach for controlling pest insects and pathogenic fungi. The ability of chitinases to attack and digest chitin in the peritrophic matrix or exoskeleton raises the possibility to use them as insect control method. In this study, an insect chitinase cDNA from cotton leaf worm (Spodoptera littoralis) has been synthesized. Transgenic maize plant system was used to improve its tolerance against insects. Insect chitinase transcripts and proteins were expressed in transgenic maize plants. The functional integrity and expression of chitinase in progenies of the transgenic plants were confirmed by insect bioassays. The bioassays using transgenic corn plants against corn borer (Sesamia cretica) revealed that ~50% of the insects reared on transgenic corn plants died, suggesting that transgenic maize plants have enhanced resistance against S. cretica.

  20. Meiotic behavior as a selection tool in silage corn breeding.

    Science.gov (United States)

    Souza, V F; Pagliarini, M S; Scapim, C A; Rodovalho, M; Faria, M V

    2010-10-19

    In breeding programs, commercial hybrids are frequently used as a source of inbred lines to obtain new hybrids. Considering that maize production is dependent on viable gametes, the selection of populations to obtain inbred lines with high meiotic stability could contribute to the formation of new silage corn hybrids adapted to specific region. We evaluated the meiotic stability of five commercial hybrids of silage corn used in southern Brazil with conventional squashing methods. All of them showed meiotic abnormalities. Some abnormalities, such as abnormal chromosome segregation and absence of cytokinesis, occurred in all the genotypes, while others, including cytomixis and abnormal spindle orientation, were found only in some genotypes. The hybrid SG6010 had the lowest mean frequency of abnormal cells (21.27%); the highest frequency was found in the hybrid P30K64 (44.43%). However, the frequency of abnormal meiotic products was much lower in most genotypes, ranging from 7.63% in the hybrid CD304 to 43.86% in Garra. Taking into account the percentage of abnormal meiotic products and, hence, meiotic stability, only the hybrids CD304, P30K64, SG6010, and P30F53 are recommended to be retained in the breeding program to obtain inbred lines to create new hybrids.

  1. Acceptability of amaranth grain-based nutritious complementary foods with dagaa fish (Rastrineobola argentea) and edible termites (Macrotermes subhylanus) compared to ‘Corn-Soy-Blend Plus’ among young children/mothers dyads in Western Kenya

    DEFF Research Database (Denmark)

    Konyole, Silvenius O.; Kinyuru, John N.; Owuor, Bethwell O.

    2012-01-01

    We assessed acceptability of two flours and porridges of complementary foods based on germinated grain amaranth and maize with or without edible termites and dagaa small fish named "Winfood Classic" (WFC) and "Winfood Lite" (WFL), respectively, compared to Corn Soy Blend Plus (CSB+) among mothers...

  2. Acceptability of amaranth grain-based nutritious complementary foods with dagaa fish (Rastrineobola argentea) and edible termites (Macrotermes subhylanus) compared to ‘Corn-Soy-Blend Plus’ among young children/mothers dyads in Western Kenya

    DEFF Research Database (Denmark)

    Konyole, Silvenius O.; Kinyuru, John N.; Owuor, Bethwell O.

    2012-01-01

    We assessed acceptability of two flours and porridges of complementary foods based on germinated grain amaranth and maize with or without edible termites and dagaa small fish named "Winfood Classic" (WFC) and "Winfood Lite" (WFL), respectively, compared to Corn Soy Blend Plus (CSB+) among mothers...

  3. Design and Performance Evaluation of a Corn De-Cobbing and Separating Machine

    Directory of Open Access Journals (Sweden)

    Oriaku E.C

    2014-06-01

    Full Text Available Agricultural products like maize, soya bean, millet and rice, when processed into quality forms not only prolongs the useful life of these products, but increases the net profit farmers make from mechanization technologies of such products. One of the most important processing operations done to bring out the quality of maize is de-cobbing or threshing of maize. Consequently, a de-cobbing and separation machine was designed, fabricated and its performance evaluated. Corn at moisture content of 15.14% db sourced locally was used in the experiment and the data collected were analyzed. Results showed that for a total 20kg of sample tested, the average feed and threshing time were 2.37 and 2.95 minutes respectively. The average feed and threshing rates were 2.06 and 1.65 kg/min with an average threshing efficiency of 78.93 %. The average separation efficiency was 56.06 %. These results indicate that threshing and separation can be performed out satisfactorily with the designed machine and it can be used to process about 1 tonne of maize per nine-hour shift.

  4. Fact sheet: Ethanol from corn

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-05-31

    This fact sheet is intended to provide an overview of the advantages of ethanol from corn, emphasizing ethanol`s contribution to environmental protection and sustainable agriculture. Ethanol, an alternative fuel used as an octane enhancer is produced through the conversion of starch to sugars by enzymes, and fermentation of these sugars to ethanol by yeast. The production process may involve wet milling or dry milling. Both these processes produce valuable by-products, in addition to ethanol and carbon dioxide. Ethanol contains about 32,000 BTU per litre. It is commonly believed that using state-of-the-art corn farming and corn processing processes, the amount of energy contained in ethanol and its by-products would be more than twice the energy required to grow and process corn into ethanol. Ethanol represents the third largest market for Ontario corn, after direct use as animal feed and wet milling for starch, corn sweetener and corn oil. The environmental consequences of using ethanol are very significant. It is estimated that a 10 per cent ethanol blend in gasoline would result in a 25 to 30 per cent decrease in carbon monoxide emissions, a 6 to 10 per cent decrease in net carbon dioxide, a slight increase in nitrous oxide emissions which, however, would still result in an overall decrease in ozone formation, since the significant reduction in carbon monoxide emissions would compensate for any slight increase in nitrous oxide. Volatile organic compounds emission would also decrease by about 7 per cent with a 10 per cent ethanol blend. High level blends could reduce VOCs production by as much as 30 per cent. 7 refs.

  5. Breeding of maize types with specific traits at the Maize Research Institute, Zemun Polje

    Directory of Open Access Journals (Sweden)

    Pajić Zorica

    2007-01-01

    Full Text Available Maize is primarily grown as an energy crop, but the use of different specific versions, such as high-oil maize, high-lysine maize, waxy maize, white-seeded maize, popping maize and sweet maize, is quite extensive. Speciality maize, due to its traits and genetic control of these traits, requires a particular attention in handling breeding material during the processes of breeding. It is especially related to prevention of uncontrolled pollination. In order to provide successful selection for a certain trait, the following specific procedures in evaluation of the trait are necessary: the estimation of a popping volume and flake quality in popping maize; the determination of sugars and harvest maturity in sweet maize; the determination of oil in selected samples of high-oil maize types, and so forth. Breeding programmes for speciality maize, except high-amylose maize, have been implemented at the Maize Research Institute, Zemun Polje, Belgrade, for the last 45 years. A great number of high-yielding sweet maize hybrids, popping maize, high-oil and high-lysine, flint and white-seeded maize hybrids were developed during this 45-year period. Auspicious selection and breeding for these traits is facilitated by the abundant genetic variability and technical and technological possibilities necessary for successful selection.

  6. Influence factor analysis of mechanical damage on corn ear picking%玉米摘穗收获机械损伤影响因素分析

    Institute of Scientific and Technical Information of China (English)

    耿爱军; 杨建宁; 张姬; 张智龙; 杨启勇; 李汝莘

    2016-01-01

    increase of the corn ear, the center of gravity for corn ears was upward and the corn ear swing increased when it was picked, the probability of collision increased. The rate of corn damage descended as the rotating speed of stripping roller changed from 650 to 800 r/min. When the rotating speed of stripping roller was more than 800 r/min, the corn damage increased significantly. When the rotation of stripping roller became faster, the down velocity of corn ears became bigger, the impact between corn ear and stripping roller increased, and the corn damage rose. Besides, the reduction of friction between the maize straw and stripping roller was another reason for the increasing of corn damage. The corn seed loss rate decreased and then increased as the rotating speed of stripping roller changed from 650 to 750 r/min, and the corn seed loss rate decreased again as the rotating speed of stripping roller changed from 750 to 850 r/min. Because when the rotation of stripping roller became faster, the corn ear down speed became bigger, the impact between the corn ear and stripping roller increased. The corn damage and seed loss decreased and then increased as the stripping roller clearance changed from 8 to 15 mm. The corn damage and seed loss were the minimum when the stripping roller clearance was 10 mm, and then with the clearance of stripping roller increasing, the corn damage and seed loss ascended, which was consistent with the result of theoretical analysis. And the mechanical damage form of corn usually included corn nibbled, corn impacted, corn extruded and broken corn ear. The process of corn ear picking was recorded with the high-speed camera and the movement of corn ear was analyzed. The results indicated that the moving velocity of corn ear had no effect on corn damage. It was also observed from the record that the moving state of corn ear picking had 3 types: first, corn ear was picked smoothly and entered the conveyer device; second, corn ear bounced; third, corn

  7. Seed biopriming with novel strain of Trichoderma harzianum for the control of toxigenic Fusarium verticillioides and fumonisins in maize

    DEFF Research Database (Denmark)

    Nayaka, S.Chandra; Niranjana, S.R.; Shankar, A.C. Uday;

    2010-01-01

    Fusarium verticillioides is one of the most important fungal pathogens in maize causing both pre- and post-harvest losses and also capable of producing Fumonisins. In the present study attempts have been made for screening potential T. harzianum from native rhizosphere and to study its effect...... on Fusarium ear rot disease, fumonisin accumulation in different maize cultivars grown in India. Eight isolates of T. harzianum were isolated and T. harzianum isolate Th-8 exhibited better antifungal activity than carbendizim. Th-8 was formulated in different solid substrates like wheat bran, paddy husk......, talcum powder and cornstarch. Maize seeds of kanchan (moderately resistant), pioneer (resistant) and sweet corn (susceptible) were selected for laboratory and field studies and these seeds were treated with a conidial suspension of T. harzianum at the rate of 1   108 spore/ml and formulation at the rate...

  8. Multicolored sweet-waxy corn variety-Caitiannuo 1

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    @@ Corn, as the third largest grain crop in China, isgrown on approximately 200 million ha per year. With theimprovement of people's living standard, variousrequirements for corn breeding have presented. Nowadays,the aim of corn breeding is not only for increasing yield,but also for higher quality and other special demands, suchas sweet corn, waxy corn, colored corn, oil-rich corn,lysine-rich corn, etc.

  9. BROWSES ENSILED WITH MAIZE FODDER

    African Journals Online (AJOL)

    Leucaenalmoae., respectively. Gliricidia/maize silages exhibited the best fermentation and nutrient patterns. .... proteolysis or deamination of protein in the silages. The DM losses ..... of legume shrubs and trees as fodders for livestockin the ...

  10. Evaluation of maize inbred lines currently used in Chinese breeding programs for resistance to six foliar diseases

    Institute of Scientific and Technical Information of China (English)

    Xiaoming; Wang; Yunhua; Zhang; Xiude; Xu; Hongjie; Li; Xiaofei; Wu; Shihuang; Zhang; Xinhai; Li

    2014-01-01

    Foliar diseases are common in most maize-producing regions and have caused serious yield reduction in China. To evaluate genetic resistance of parental lines actively used in maize breeding programs to major foliar diseases, 152 maize inbred lines were tested against northern corn leaf blight(NCLB), southern corn leaf blight(SCLB), Curvularia leaf spot(CLS),gray leaf spot(GLS), common rust, and southern rust from 2003 to 2005. A small number of lines exhibited highly resistant reactions to common rust and southern rust, but none were highly resistant to NCLB, SCLB, CLS, and GLS. Although 53.3%, 40.8%, and 80.7% of lines were resistant to NCLB, SCLB, and common rust, the resistance in most lines was moderate.Resistance to CLS, GLS, and southern rust was rare in this collection of maize lines. Five lines,313, Chang 7-2, Qi 319, Qi 318, and Shen 137, were resistant to five diseases tested. Lines belonging to heterotic subgroup PB exhibited better resistance to the foliar diseases than lines from other heterotic subgroups, such as BSSS, PA, Lancaster, LRC, and PA. The results will be of benefit to breeders for selecting lines in disease resistance breeding programs.

  11. Influence of corn oil recovery on life-cycle greenhouse gas emissions of corn ethanol and corn oil biodiesel

    OpenAIRE

    Wang, Zhichao; Dunn, Jennifer B.; Han, Jeongwoo; Wang, Michael Q.

    2015-01-01

    Background Corn oil recovery and conversion to biodiesel has been widely adopted at corn ethanol plants recently. The US EPA has projected 2.6 billion liters of biodiesel will be produced from corn oil in 2022. Corn oil biodiesel may qualify for federal renewable identification number (RIN) credits under the Renewable Fuel Standard, as well as for low greenhouse gas (GHG) emission intensity credits under California’s Low Carbon Fuel Standard. Because multiple products [ethanol, biodiesel, and...

  12. 9 CFR 319.102 - Corned beef round and other corned beef cuts.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Corned beef round and other corned beef cuts. 319.102 Section 319.102 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... Meats, Unsmoked and Smoked § 319.102 Corned beef round and other corned beef cuts. In preparing...

  13. Corn earworm (Lepidoptera: Noctuidae) in northeastern field corn: infestation levels and the value of transgenic hybrids.

    Science.gov (United States)

    Bohnenblust, Eric; Breining, Jim; Fleischer, Shelby; Roth, Gregory; Tooker, John

    2013-06-01

    Corn earworm, Helicoverpa zea (Boddie), is a polyphagous noctuid pest of agricultural crops across the United States that is gaining attention as a pest of field corn. Before the introduction of transgenic insect-resistant hybrids, this pest was largely ignored in field corn, but now many Bacillus thuringiensis (Bt) corn hybrids have activity against corn earworm. However, the value of control in the northeastern United States is unclear because the risk posed by corn earworm to field corn has not been well characterized. To understand the threat from corn earworm and the value of Bt hybrids in field corn, we assessed corn earworm injury in Bt and non-Bt hybrids at 16 sites across four maturity zones throughout Pennsylvania in 2010, and 10 sites in 2011. We also used corn earworm captures from the PestWatch pheromone trapping network to relate moth activity to larval damage in field corn. Corn earworm damage was less than one kernel per ear at 21 of 26 sites over both years, and the percentage of ears damaged was generally corn earworm damage relative to non-Bt hybrids, but we found no differences among Bt traits. Cumulative moth captures through July effectively predicted damage at the end of the season. Currently, the additional benefit of corn earworm control provided by Bt hybrids is typically less than US$4.00/ha in northeastern field corn.

  14. Sequencing the maize genome.

    Science.gov (United States)

    Martienssen, Robert A; Rabinowicz, Pablo D; O'Shaughnessy, Andrew; McCombie, W Richard

    2004-04-01

    Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x coverage of gene-rich regions, accompanied by less than 1x coverage of subclones from BAC contigs, will generate high-quality mapped sequence that meets the needs of geneticists while accommodating unusually high levels of structural polymorphism. By sequencing several inbred strains, we propose a strategy for capturing this polymorphism to investigate hybrid vigor or heterosis.

  15. Transgenic corn for control of the European corn borer and corn rootworms: a survey of Midwestern farmers' practices and perceptions.

    Science.gov (United States)

    Wilson, Ted A; Rice, Marlin E; Tollefson, Jon J; Pilcher, Clinton D

    2005-04-01

    In 2001, a self-administered questionnaire was sent to 1000 corn, Zea mays L., farmers in each of five states (Illinois, Indiana, Iowa, Minnesota, and Nebraska) to evaluate their perceptions of transgenic corn designed to control the European corn borer, Ostrinia nubilalis (Hübner), and corn rootworms, Diabrotica spp. Respondents returned 1,313 surveys (26.2%). Farmers with small acreages planted a greater portion of their corn (54.5%) with transgenic corn for control of European corn borer than farmers with large farms (39.2%). The majority (75.2%) of farmers use crop rotation to control the corn rootworm. Nine insecticides comprised 92.2% of the commercial soil insecticides used for control of corn rootworm larvae. More than one-third of the farmers in Illinois (33.5%) and Indiana (39.4%) treated first-year corn for corn rootworm, primarily due to western corn rootworm egg laying in soybean, Glycine max (L.). When asked whether they would plant transgenic corn protected against the corn rootworm, 35.0% of farmers responded they would, whereas 40.5% said they were unsure. The two greatest farmer concerns about transgenic corn were the ability to sell harvested grain (59.3%) and additional technology fees (54.8%). Respondents indicated that less farmer exposure to insecticide (69.9%) and less insecticide in the environment (68.5%) were the primary benefits of transgenic corn. Farmers who had no concerns about transgenic corn for rootworm control were more likely to purchase the product (46.8%). The most common refuge-planting options farmers favored were adjacent fields (30.9%) and split fields (29.9%). Farmers (21.1%) observed a yield increase (23.7 bu/ha [9.6 bu/acre]) when using transgenic corn for European corn borer control compared with non-transgenic corn. These data can help in understanding farmers' knowledge and concerns regarding transgenic corn. This information may be of value to guide researchers, extension specialists, and policy makers in designing

  16. Apparent in vivo nutrient digestibility of maize silages in horses

    Directory of Open Access Journals (Sweden)

    Adriana PÍŠOVÁ

    2016-12-01

    Full Text Available The aim of the experiment was to analyzed coefficients of digestibility of the maize silage nutrients in horse feeding. Horses of Slovak warmblood breed engaged in the research were 2.5 ± 0.5 years old. Horses in boxes without bedding to avoid eating it and excrement contamination were stabled. Horses individually with ad libitum access to water were housed. The boxes with trough for corn silage were equiped. The corn silage contained 362.3 g of dry matter, 74.1 g of crude protein, 27.1 g of crude fat and 948.2 g of organic matter in kg of dry matter. The feed twice a day, in the morning and in the evening was given. The feed intake and leftovers were weighed every day. Excrements immediately after excreting to avoid urine contamination into plastic containers were collected and weighed. The content of nutrients in the feed intake and in the excrements were analysed. In analysed samples the gravimetric dry matter amount by drying at 103 ± 2 °C, crude protein content by Kjeldahl method, fat content by extraction method according to Soxhlett – Henkel, ash content by burning the sample at 530 ± 20 °C in muffle furnace and organic matter content by calculation were determined. Coefficients of apparent digestibility in percent from taken in and excreted nutrients were calculated. In maize silage for horse feeding an average coefficient of digestibility of dry matter 61.94%, of crude protein 56.03%, of crude fat 72.34% and of organic matter 65.19% was found.

  17. Chemical Composition Profile of Canned and Frozen Sweet Corn (Zea mays L. in Iran

    Directory of Open Access Journals (Sweden)

    Akram Ahmadi

    2015-06-01

    Full Text Available Maize is one of the oldest and most productive popular grains and can be consumed as food at various developmental stages from baby corn to mature grain. The maize can be processed in different products at traditional level as well as industrial scale. Due to vast corn consumption by people in this research the concentration of histamine ,some trace and essential mineral and also heavy metals has been determined in 120 samples of popular brands of canned and frozen sweet corn in 2014. These samples (60 of them were canned corn and the rest were frozen were collected from different local markets of Tehran, Iran. For reliable, accurate and precise measurements, all canned and frozen samples had the same date of producing. Samples were randomly purchased for analysis and analyzed according to standardized international protocols by wet digestion method. Histamine content was determined by Capillary Electrophoresis while metals were determined by Atomic absorption spectrometer. The results obtained revealed that, the mean ranges of the elements analyzed in (μg.g-1 between the frozen and canned food are as follows: Ni (0.31 – 17.44, Zn (204.14 – 266.76, Cu (6.22 – 8.03, Ca (1611 –8557, Mg (1063 – 1208, Fe (1.98-87.23, Sn (19.01- 350.8 respectively. Some of the measured values found, not only relatively high in canned compared to frozen food samples, but also exceeds the international tolerance levels. Histamine content in 95% of samples was lower than maximum level set by WHO. According to variance analyses of data, there was a positive correlation between the storage time and heavy metal contents especially tin, zinc and iron and they were varied significantly (p ­< 0.001 after 3 months of storage .The monitoring of mineral and heavy metals in frozen and canned food samples is vital important challenge to control and improve the food industry strategies.

  18. The effect of the undigested fraction of maize products on the activity and composition of the microbiota determined in a dynamic in vitro model of the human proximal large intestine.

    NARCIS (Netherlands)

    Maathuis, A.; Hoffman, A.; Evans, A.; Sanders, L.; Venema, K.

    2009-01-01

    OBJECTIVE: To investigate the effect of 5 newly developed maize-based fibers on the activity and composition of the microbiota in the colon. The fibers tested were glucose-based and had variable structures, including 2 resistant starch preparations, soluble corn fiber, pullulan, and soluble fiber de

  19. Effect of Organic Amendments and Chemical Fertilization in Production of Corn (Zea Mays L.

    Directory of Open Access Journals (Sweden)

    Fabio Emilio Forero Ulloa

    2014-11-01

    Full Text Available Corn is grown in 135 countries, and because of its uses and nutritional benefits is the world's most important cereal. In Colombia it is grown in various agro-ecological conditions of production. The bagasse is an organic residue resulting from the grinding of sugar cane (Saccharum officinarum L., used for the production of jaggery (solid resulting of boiling and evaporation of the juice from sugar cane, which can be used as an amendment and is a soil conditioner, as a rich source of phosphorus, calcium and nitrogen. The aim of the research was to evaluate the effect of bagasse against the application of other organic sources and chemical fertilization in maize, variety ICA-V-305. For this, a completely random statistical design with four treatments and absolute control was established. Results were subjected to analysis of variance and Tukey comparison test. Applying Bagasse + Abimgra® produced the greatest number of ears of corn, while the use of only bagasse, presented the second best results in terms of number of grains / ear and weight of 100 grains of corn, therefore bagasse becomes , through time, an important option as organic amendment, which would favor the production of corn, and an option as organic fertilizer.

  20. Acidulant and oven type affect total anthocyanin content of blue corn cookies.

    Science.gov (United States)

    Li, Jian; Walker, Chuck E; Faubion, Jon M

    2011-01-15

    Anthocyanins, pink to purple water-soluble flavonoids, are naturally occurring pigments with claimed health benefits. However, they are sensitive to degradation by high pH, light and temperature. Blue corn (maize) contains high levels of anthocyanins. Cookies are popular snacks and might serve as a vehicle to deliver antioxidants. A cookie formula with a high level of blue corn was developed with added acidulents and baked in ovens with different heat transfer coefficients. The best whole-grain blue corn flour/wheat pastry flour ratio (80:20 w/w), guar gum level (10 g kg(-1), flour weight basis) and water level (215 g kg(-1), flour weight basis) were determined based on response surface methodology analysis. The interactions of citric and lactic acids and glucono-δ-lactone with three oven types having different heat transfer coefficients (impingement oven 179 °C/4 min, reel oven 204 °C/10 min and convection oven 182 °C/4 min) influenced the total anthocyanin content (TAC) remaining in blue corn-containing cookies after baking. Cookies baked with citric acid in the convection oven retained the maximum TAC (227 ± 3 mg kg(-1)). By baking rapidly at lower temperatures and adding acidulents, it may be possible to increase residual natural source antioxidants in baked foods. Copyright © 2010 Society of Chemical Industry.

  1. Weed Diversity Affects Soybean and Maize Yield in a Long Term Experiment in Michigan, USA.

    Science.gov (United States)

    Ferrero, Rosana; Lima, Mauricio; Davis, Adam S; Gonzalez-Andujar, Jose L

    2017-01-01

    Managing production environments in ways that promote weed community diversity may enhance both crop production and the development of a more sustainable agriculture. This study analyzed data of productivity of maize (corn) and soybean in plots in the Main Cropping System Experiment (MCSE) at the W. K. Kellogg Biological Station Long-Term Ecological Research (KBS-LTER) in Michigan, USA, from 1996 to 2011. We used models derived from population ecology to explore how weed diversity, temperature, and precipitation interact with crop yields. Using three types of models that considered internal and external (climate and weeds) factors, with additive or non-linear variants, we found that changes in weed diversity were associated with changes in rates of crop yield increase over time for both maize and soybeans. The intrinsic capacity for soybean yield increase in response to the environment was greater under more diverse weed communities. Soybean production risks were greatest in the least weed diverse systems, in which each weed species lost was associated with progressively greater crop yield losses. Managing for weed community diversity, while suppressing dominant, highly competitive weeds, may be a helpful strategy for supporting long term increases in soybean productivity. In maize, there was a negative and non-additive response of yields to the interaction between weed diversity and minimum air temperatures. When cold temperatures constrained potential maize productivity through limited resources, negative interactions with weed diversity became more pronounced. We suggest that: (1) maize was less competitive in cold years allowing higher weed diversity and the dominance of some weed species; or (2) that cold years resulted in increased weed richness and prevalence of competitive weeds, thus reducing crop yields. Therefore, we propose to control dominant weed species especially in the years of low yield and extreme minimum temperatures to improve maize yields

  2. The Reaction of some Maize Hybrids, Created at ARDS TURDA, to Fusarium spp. Infection

    Directory of Open Access Journals (Sweden)

    Laura ȘOPTEREAN

    2017-05-01

    Full Text Available The most important disease of maize in Romania are stalk and ear rot, which caused yield losses in average of 20%. The resistant hibrids represent one of the most efficient solution for reducing the field loses caused by Fusarium spp. on the maize (Nagy et al., 2006. Diseases caused by Fusarium spp. can affect the yield and grain quality of maize because of contamination with numerous mycotoxins produced by these fungi (Czembor et al., 2015. The purpose of this paper was to know more about the reaction of different maize hybrids to Fusarium and the evaluating the effect of ear rot on the yield ability and mycotoxins accumulation. The experiments carried out at ARDS Turda, during four years (2012-2015. The biological material was represented by 8 hybrids, from different maturity groups, tested in two infection conditions with Fusarium spp. (natural and artificial infections. The temperature and rainfalls of the four years of experiments corresponding to the vegetation of maize (april-september are influenced favourably the pathogenesis of stalk and ear rot caused by Fusarium spp. and a good discrimination of the resistance reaction of genotypes. Fusarium ear rot has significantly affected production capacity and chemical composition of corn hybrids tested. In conditions of artificial infection with Fusarium spp. was a decrease in the content of starch, fat and increased protein content compared with artificially inoculated variants. The quantity of fumonizin B1+B2 has reached to 5630 μg/kg in conditions of artificial infection. There are negative correlations between production capacity and degree of attack of fusarium ear rot; depending on the reacting genotypes tested increasing disease causes production decrease. The response of maize hybrids to Fusarium infection is influenced by infection and climatic conditions. These factors affect production both in terms of quantity and quality and accumulation of mycotoxins.

  3. A Classification of 45 Maize Inbred Lines Used in China with RFLP Markers

    Institute of Scientific and Technical Information of China (English)

    HUANG Yi-qin; LI Jian-sheng

    2002-01-01

    The classification of heterotic groups is essential to maize breeding because knowledge of heterotic groups could be interest to both the combination of outstanding hybrids and the improvement of elite inbred lines. RFLP has provided a powerful tool to assign maize inbred lines into heterotic groups. In this investigation, 45 inbred lines, used widely in south and southwest China, were chosen for RFLP analysis,among which 4 lines came from American, representing different heterotic groups in U.S. corn belt. 54 RFLP core markers covering 10 chromosomes of maize were used. A total DNA of each sample was digested with EcoR I, BamH Ⅰ and Hind Ⅲ . The procedure of RFLP was employed as described by a manual from maize RFLP lab at University of Missouri, Columbia. A total of 860 bands were detected among 45 inbred lines based on RFLP analysis, which were involved in 212 loci. Alleles at each locus ranged from 2 to 9 with an average of 4.06. In total, The 45 inbred lines were classified into 6 heterotic groups according to RFLP data with Ward's method. 3 heterotic groups, including Mo17, B73 and Oh43 respectively, seemed to be the same to U. S. heterotic groups. 21 inbred lines, most of which derived from Chinese local germplasm, were classified together into two heterotic groups, indicating domistic germplasm was different from U. S.germplasm at the molecular level and played an important role in maize hybrid production in China. Two inbred lines from tropic germplasm were assigned in the same group. These results provided useful information for our understanding maize heterotic groups and heterotic patterns in China.

  4. Weed Diversity Affects Soybean and Maize Yield in a Long Term Experiment in Michigan, USA

    Science.gov (United States)

    Ferrero, Rosana; Lima, Mauricio; Davis, Adam S.; Gonzalez-Andujar, Jose L.

    2017-01-01

    Managing production environments in ways that promote weed community diversity may enhance both crop production and the development of a more sustainable agriculture. This study analyzed data of productivity of maize (corn) and soybean in plots in the Main Cropping System Experiment (MCSE) at the W. K. Kellogg Biological Station Long-Term Ecological Research (KBS-LTER) in Michigan, USA, from 1996 to 2011. We used models derived from population ecology to explore how weed diversity, temperature, and precipitation interact with crop yields. Using three types of models that considered internal and external (climate and weeds) factors, with additive or non-linear variants, we found that changes in weed diversity were associated with changes in rates of crop yield increase over time for both maize and soybeans. The intrinsic capacity for soybean yield increase in response to the environment was greater under more diverse weed communities. Soybean production risks were greatest in the least weed diverse systems, in which each weed species lost was associated with progressively greater crop yield losses. Managing for weed community diversity, while suppressing dominant, highly competitive weeds, may be a helpful strategy for supporting long term increases in soybean productivity. In maize, there was a negative and non-additive response of yields to the interaction between weed diversity and minimum air temperatures. When cold temperatures constrained potential maize productivity through limited resources, negative interactions with weed diversity became more pronounced. We suggest that: (1) maize was less competitive in cold years allowing higher weed diversity and the dominance of some weed species; or (2) that cold years resulted in increased weed richness and prevalence of competitive weeds, thus reducing crop yields. Therefore, we propose to control dominant weed species especially in the years of low yield and extreme minimum temperatures to improve maize yields

  5. Geographic information systems in corn rootworm management

    Science.gov (United States)

    Corn rootworms (Diabrotica spp. Coleoptera: Chrysomelidae) are serious pests of corn (Zea mays) in the United States and Europe. Control measures for corn rootworms (CRW) were historically based upon chemical pesticides and crop rotation. Pesticide use created environmental and economic concerns. In...

  6. Influence of maize/lablab intercropping on lepidopterous stem borer infestation in maize.

    Science.gov (United States)

    Maluleke, Mary H; Addo-Bediako, Abraham; Ayisi, Kingsley K

    2005-04-01

    Lepidopterous stem borers seriously affect production of maize, Zea mays L., in sub-Saharan Africa. Intercropping maize with legumes such as lablab, Lablab purpurens (L.), is one of the effective systems to control stem borers. Sole culture maize and maize/lablab intercrop system of different lablab densities were planted at two locations to investigate the effects of intercrop system on incidence and severity of stem borers with particular reference to Chilo partellus (Swinhoe) (Lepidoptera: Pyralidae). Stem borer infestation was found to be more severe in sole culture maize than maize in maize/lablab intercrop. There was a significantly negative relationship between lablab densities and maize grain yields, suggesting a possible competition for resources between the two crops. It was concluded that density of lablab and date of planting of lablab in maize/lablab intercropping have significant affects on stem borer populations and maize grain yields.

  7. IMAZAPYR-RESISTANT MAIZE TECHNOLOGY ADOPTION FOR ...

    African Journals Online (AJOL)

    Prof. Adipala Ekwamu

    decisions by protecting maize (Zea mays L.) crop in western Kenya from Striga. Key Words: .... Imazapyr-resistant maize technology adoption for witch weed control. 175. Areas” (IAs) and ..... Iowa State University Press, Ames,. IA, USA. 164p.

  8. Utilisation of corn (Zea mays) bran and corn fiber in the production of food components.

    Science.gov (United States)

    Rose, Devin J; Inglett, George E; Liu, Sean X

    2010-04-30

    The milling of corn for the production of food constituents results in a number of low-value co-products. Two of the major co-products produced by this operation are corn bran and corn fiber, which currently have low commercial value. This review focuses on current and prospective research surrounding the utilization of corn fiber and corn bran in the production of potentially higher-value food components. Corn bran and corn fiber contain potentially useful components that may be harvested through physical, chemical or enzymatic means for the production of food ingredients or additives, including corn fiber oil, corn fiber gum, cellulosic fiber gels, xylo-oligosaccharides and ferulic acid. Components of corn bran and corn fiber may also be converted to food chemicals such as vanillin and xylitol. Commercialization of processes for the isolation or production of food products from corn bran or corn fiber has been met with numerous technical challenges, therefore further research that improves the production of these components from corn bran or corn fiber is needed.

  9. Biology and natural enemies of Peregrinus maidis (Ashmead in corn (Zea mays L., in systems of polycrops

    Directory of Open Access Journals (Sweden)

    Marcos Tulio García González

    2015-02-01

    Full Text Available This research was done in Fomento municipality, Sancti Spíritus, in Cuba, from 2011 through 2013 in two ecosystems and in two sowing times (rainy and dry seasons. The objectives were to describe the life cycle of Peregrinus maidis (Ashmead and the damage caused to corn (Zea mays L., to identify the main pest bio-regulators naturally occurring in two agro-ecosystems, and to determine the predator-prey relationship. The experiment was arranged in a randomized block design. The data were collected through observation at weekly intervals. In order to estimate the number of pest insects and bio-regulators occurring on the plants, 25 plants per parcel were selected for a total of 100 plants per treatment (maize/squash, maize/beans, maize/ Sesame, maize/sunflower, and maize monoculture. Once the research was accomplished, it was concluded that P maidis had been present since the first week, and reached its population peak in week six. The lower indexes of affectation occurred in the pre-mountain agro-ecosystems, and with more frequency in the maize/sesame and maize/squash polyculture, which depended on the time of the year and the season. Fifteen species of P. maidis control insects of were identified, and Scymnus sp was found in all treatments. The life cycle of P maidis averaged 5.4 days more in the dry season than in rain season. The predator-prey relationship showed relatively superior indexes in the polyculture systems than in monocrops systems.

  10. Ustilago maydis reprograms cell proliferation in maize anthers.

    Science.gov (United States)

    Gao, Li; Kelliher, Timothy; Nguyen, Linda; Walbot, Virginia

    2013-09-01

    The basidiomycete Ustilago maydis is a ubiquitous pathogen of maize (Zea mays), one of the world's most important cereal crops. Infection by this smut fungus triggers tumor formation in aerial plant parts within which the fungus sporulates. Using confocal microscopy to track U. maydis infection on corn anthers for 7 days post-injection, we found that U. maydis is located on the epidermis during the first 2 days, and has reached all anther lobe cell types by 3 days post-injection. Fungal infection alters cell-fate specification events, cell division patterns, host cell expansion and host cell senescence, depending on the developmental stage and cell type. Fungal effects on tassel and plant growth were also quantified. Transcriptome profiling using a dual organism microarray identified thousands of anther genes affected by fungal infection at 3 days post-injection during the cell-fate specification and rapid cell proliferation phases of anther development. In total, 4147 (17%) of anther-expressed genes were altered by infection, 2018 fungal genes were expressed in anthers, and 206 fungal secretome genes may be anther-specific. The results confirm that U. maydis deploys distinct genes to cause disease in specific maize organs, and suggest mechanisms by which the host plant is manipulated to generate a tumor.

  11. Enhancement of resistance to aphids by introducing the snowdrop lectin gene gna into maize plants.

    Science.gov (United States)

    Wang, Zhaoyu; Zhang, Kewei; Sun, Xiaofen; Tang, Kexuan; Zhang, Juren

    2005-12-01

    In order to enhance the resistance to pests, transgenic maize (Zea mays L.) plants from elite inbred lines containing the gene encoding snowdrop lectin (Galanthus nivalis L. agglutinin; GNA) under control of a phloem-specific promoter were generated through the Agrobacterium tumefaciens-mediated method. The toxicity of GNA-expressing plants to aphids has also been studied. The independently derived plants were subjected to molecular analyses. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that the gna gene was integrated into maize genome and inherited to the following generations. The typical Mendelian patterns of inheritance occurred in most cases. The level of GNA expression at 0.13%-0.28% of total soluble protein was observed in different transgenic plants. The progeny of nine GNA-expressing independent transformants that were derived separately from the elite inbred lines DH4866, DH9942, and 8902, were selected for examination of resistance to aphids. These plants synthesized GNA at levels above 0.22% total soluble protein, and enhanced resistance to aphids was demonstrated by exposing the plants to corn leaf aphid (Rhopalosiphum maidis Fitch) under greenhouse conditions. The nymph production was significantly reduced by 46.9% on GNA-expressing plants. Field evaluation of the transgenic plants supported the results from the inoculation trial. After a series of artificial self-crosses, some homozygous transgenic maize lines expressing GNA were obtained. In the present study, we have obtained new insect-resistant maize material for further breeding work.

  12. Seeds of the Wild Progenitor of Maize Possess Bacteria That Antagonize Foodborne Pathogens.

    Science.gov (United States)

    Shehata, Hanan R; Griffiths, Mansel W; Raizada, Manish N

    2017-02-10

    Endophytes are microorganisms that inhabit plant tissues without causing disease. Some endophytes help their hosts to combat pathogens. Here we explored the hypothesis that the plant-derived foods consumed by humans and other animals host endophytes that also antagonize foodborne pathogens or food-rotting agents. Our laboratory previously cultured a library of bacterial endophytes from different members of the maize/corn family (Zea) including wild relatives. Here, 190 of these endophytes were screened for their ability to antagonize four foodborne pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Clostridium perfringens, and Salmonella enterica Newport) and a food spoiling agent (Pseudomonas fluorescens) using dual culture assays. Two Paenibacillus polymyxa endophytes (strains 3C6 and 3G11) were found to inhibit the growth of all five deleterious strains on agar. Using conserved polymerase chain reaction primers and sequencing, both beneficial endophytes were found to encode polymyxin genes, suggesting a potential antibacterial mechanism of action. Polymyxin production by both strains was confirmed using enzyme-linked immunosorbent assay. Strains 3C6 and 3G11 originated, respectively, from the seeds of the wild Central American maize species Zea diploperennis, and the wild ancestor of modern maize, Zea mays ssp parviglumis (Parviglumis). As the latter is the direct ancestor of modern maize, we discuss the role its endophyte(s) may have played in promoting crop domestication by suppressing foodborne pathogens and/or food-spoilage agents.

  13. Enhancement of resistance to aphids by introducing the snowdrop lectin gene gna into maize plants

    Indian Academy of Sciences (India)

    Zhaoyu Wang; Kewei Zhang; Xiaofen Sun; Kexuan Tang; Juren Zhang

    2005-12-01

    In order to enhance the resistance to pests, transgenic maize (Zea mays L.) plants from elite inbred lines containing the gene encoding snowdrop lectin (Galanthus nivalis L. agglutinin; GNA) under control of a phloemspecific promoter were generated through the Agrobacterium tumefaciens-mediated method. The toxicity of GNA-expressing plants to aphids has also been studied. The independently derived plants were subjected to molecular analyses. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that the gna gene was integrated into maize genome and inherited to the following generations. The typical Mendelian patterns of inheritance occurred in most cases. The level of GNA expression at 0.13%–0.28% of total soluble protein was observed in different transgenic plants. The progeny of nine GNA-expressing independent transformants that were derived separately from the elite inbred lines DH4866, DH9942, and 8902, were selected for examination of resistance to aphids. These plants synthesized GNA at levels above 0.22% total soluble protein, and enhanced resistance to aphids was demonstrated by exposing the plants to corn leaf aphid (Rhopalosiphum maidis Fitch) under greenhouse conditions. The nymph production was significantly reduced by 46.9% on GNA-expressing plants. Field evaluation of the transgenic plants supported the results from the inoculation trial. After a series of artificial self-crosses, some homozygous transgenic maize lines expressing GNA were obtained. In the present study, we have obtained new insect-resistant maize material for further breeding work.

  14. Association of secondary traits with yield in maize F 1 's

    Directory of Open Access Journals (Sweden)

    Maicon Nardino

    2016-01-01

    Full Text Available ABSTRACT: The objective was to identify phenotypic and genotypic associations, and cause-and-effect relations of secondary components on primary components to establish criteria in the indirect selection process for maize. Partial diallel crosses were conducted in Clevelândia. F1's were evaluated in five environments. For the purpose of increasing the yield of corn grain, breeders should seek to reduce the characters distance from the last node to the first branch of the tassel, tassel length and number of branches. The indirect selection for distance from the last node to the first branch of the tassel would be effective to increase the grain yield. The selection for smaller leaf angle, larger stem diameter and thousand grain weight are favorable for increasing grain yield in maize.

  15. Molecular characterization of local maize varieties from the Biosphere Reserve La Sepultura, Mexico.

    Directory of Open Access Journals (Sweden)

    Manuel Antonio Hernández-Ramos

    2016-12-01

    Full Text Available The objective of this research was the molecular and phylogenetic characterization of local maize populations of the La Sepultura Biosphere Reserve (REBISE, Mexico. In nine communities from the REBISE, Mexico, nineteen populations of local maize were sampled during 2012-2013. Two improved commercial varieties (Vs-536 and V-424, a commercial hybrid (H-MX3, a local corn (Coastal recently introduced in the REBISE, two local materials from the state of Tlaxcala, Mexico; both from Chalqueño race and conical Elotes (young corns and a teosinte (Zea mays ssp. Mexicana were included. They totaled twenty six different samples. The characterization was done with the PCR technique and inter-type microsatellite genetic markers. In total 113, fragments were ampli ed and that ranged from 150-2200 bp, of which 85,8% were polymorphic. Sequences “AG”, “AC” and “GAA” showed a greater number of ampli ed bands and higher polymorphism. The primers comprised of UBC834, I9 and UBC868 ampli ed the best. The similarities found within the polymorphic bands may be due to their equality such as varieties, races, species or genetic combination effect, a result of their cross- pollination. The dendrogram obtained showed a relatively low cophenetic correlation (r= 0,721, with a low degree of reliability, however four major groups of varieties are clearly conformed. The rst was named Valles Altos Corns, integrated by the conical Chalqueño and the conical Elotes races; the second is identi ed as having a common ancestor with Teocintle; the third is an improved hybrid by the H-MX3; and the fourth includes the REBISE local maize varieties and other commercial varieties (Vs-536 y Vs-424.

  16. Zearalenone contamination in farm maize silage

    OpenAIRE

    L. Cavallarin; Antoniazzi, S.; E. Tabacco; G. Borreani

    2010-01-01

    Whole-plant maize silage, stored in horizontal silos, is the main diet source of lactating dairy cows in Italy. Mycotoxin contamination of maize grain has widely been described (Hussein and Brasel, 2001), while limited information is available on mycotoxins in maize silage (Oldenburg, 1991).

  17. Ingestive behavior of Nellore steers in feedlot fed with diets containing different corn hybrids

    Directory of Open Access Journals (Sweden)

    Ivone Yurika Mizubuti

    2014-02-01

    Full Text Available The objective of this work was to study the feeding behavior of Nellore beef cattle in feedlot fed with diets containing different corn hybrids. Twenty-seven animals averaging 350 ± 24 kg of body weight and 24 months of age, were used. The animals were distributed in a completely randomized design with three treatments (T, where, T1-TDFC: total diet containing flint corn, T2-TDSFC: total diet containing semi-flint corn and T3-TDSDC: total diet containing semi-dent corn, with 9 replicates per treatment. The animals were fed ad libitum twice a day (at 8:00am and 4:00pm with a isocaloric and isonitrogenous diet, with 30% of sugar cane bagasse and 70% concentrate (88% maize, 8% soybean meal, 3% mineral and vitamin supplement and 1% urea for 95 days (14 days of adaptation and 3 experimental periods of 27 days each. The animals were weighed at the beginning of the experiment and after each period of 27 days, always in a fasting period of 16 hours. The evaluation of animals feeding behavior occurred at the last day of each period by visual observation every five minutes for full periods of 24 hours. Observations were made in four shifts: morning (06:00 to 12:00, afternoon (12:00 to 18:00, evening (18:00 to 00:00 and early morning (00:00 06:00 to determine the number of ruminal bolus, chewing time, total feeding time, total ruminating standing time, total ruminating lying time, total standing idle time and total lying idle time. During the night’s observations, the stalls received artificial illumination to facilitate the data collection and the animals were adapted with light at night for three days before observations. Animals fed with diets containing semi-dent corn had longer chew time and more ruminal bolus than those fed with flint corn, but did not differ from those that received semi-flint corn in the diet. The chewing time and number of ruminal bolus varied with the observation periods, being higher in the morning and decreasing in the

  18. Improved corn protein based articles

    Science.gov (United States)

    Developing higher value uses for zein (corn protein), a potential major co-product of the bio-ethanol industry, will improve the economics of this business. Historically, zein was predominantly used in the textile fiber industry. Unfortunately the techniques used at that time to modify the zein cann...

  19. Compatibility with corn: N credits

    Science.gov (United States)

    Productive and efficient short rotations of alfalfa and corn are needed to reduce energy inputs, produce food, feed, and energy, and yield the environmental quality benefits from the perennial legume. After decades of research, however, farmers and their advisors still question how much fertility ...

  20. Maize, tropical (Zea mays L.).

    Science.gov (United States)

    Assem, Shireen K

    2015-01-01

    Maize (Zea mays L.) is the third most important food crop globally after wheat and rice. In sub-Saharan Africa, tropical maize has traditionally been the main staple of the diet; 95 % of the maize grown is consumed directly as human food and as an important source of income for the resource-poor rural population. The biotechnological approach to engineer biotic and abiotic traits implies the availability of an efficient plant transformation method. The production of genetically transformed plants depends both on the ability to integrate foreign genes into target cells and the efficiency with which plants are regenerated. Maize transformation and regeneration through immature embryo culture is the most efficient system to regenerate normal transgenic plants. However, this system is highly genotype dependent. Genotypes adapted to tropic areas are difficult to regenerate. Therefore, transformation methods used with model genotypes adapted to temperate areas are not necessarily efficient with tropical lines. Agrobacterium-mediated transformation is the method of choice since it has been first achieved in 1996. In this report, we describe a transformation method used successfully with several tropical maize lines. All the steps of transformation and regeneration are described in details. This protocol can be used with a wide variety of tropical lines. However, some modifications may be needed with recalcitrant lines.

  1. Enzymatic hydrolysis of corn bran arabinoxylan

    DEFF Research Database (Denmark)

    Agger, Jane

    This thesis concerns enzymatic hydrolysis of corn bran arabinoxylan. The work has focused on understanding the composition and structure of corn bran with specific interest in arabinoxylan with the main purpose of targeting enzymatic hydrolysis for increased yields. Corn bran has been used...... as a model substrate because it represents a readily available agroindustrial side product with upgrading potentials. Corn bran originates from the wet-milling process in corn starch processing, is the outmost layers of the corn kernel and is particularly rich in pentose monosaccharides comprising the major...... components of arabinoxylan. Corn bran is one of the most recalcitrant cereal byproducts with arabinoxylans of particular heterogeneous nature. It is also rich in feruloyl derived substitutions, which are responsible for extensive cross-linking between arabinoxylan molecules and thereby participate...

  2. Accumulation of 5-hydroxynorvaline in maize (Zea mays) leaves is induced by insect feeding and abiotic stress.

    Science.gov (United States)

    Yan, Jian; Lipka, Alexander E; Schmelz, Eric A; Buckler, Edward S; Jander, Georg

    2015-02-01

    Plants produce a wide variety of defensive metabolites to protect themselves against herbivores and pathogens. Non-protein amino acids, which are present in many plant species, can have a defensive function through their mis-incorporation during protein synthesis and/or inhibition of biosynthetic pathways in primary metabolism. 5-Hydroxynorvaline was identified in a targeted search for previously unknown non-protein amino acids in the leaves of maize (Zea mays) inbred line B73. Accumulation of this compound increases during herbivory by aphids (Rhopalosiphum maidis, corn leaf aphid) and caterpillars (Spodoptera exigua, beet armyworm), as well as in response to treatment with the plant signalling molecules methyl jasmonate, salicylic acid and abscisic acid. In contrast, ethylene signalling reduced 5-hydroxynorvaline abundance. Drought stress induced 5-hydroxynorvaline accumulation to a higher level than insect feeding or treatment with defence signalling molecules. In field-grown plants, the 5-hydroxynorvaline concentration was highest in above-ground vegetative tissue, but it was also detectable in roots and dry seeds. When 5-hydroxynorvaline was added to aphid artificial diet at concentrations similar to those found in maize leaves and stems, R. maidis reproduction was reduced, indicating that this maize metabolite may have a defensive function. Among 27 tested maize inbred lines there was a greater than 10-fold range in the accumulation of foliar 5-hydroxynorvaline. Genetic mapping populations derived from a subset of these inbred lines were used to map quantitative trait loci for 5-hydroxynorvaline accumulation to maize chromosomes 5 and 7.

  3. The U2U Corn Growing Degree Day tool: Tracking corn growth across the US Corn Belt

    Directory of Open Access Journals (Sweden)

    James R. Angel

    2017-01-01

    Full Text Available The Corn Growing Degree Day (Corn GDD tool is a web-based product that can provide decision support on a variety of issues throughout the entire growing season by integrating current conditions, historical climate data, and projections of Corn GDD through the end of the growing season based on both National Weather Service computer model forecasts and climatology. The Corn GDD tool can help agricultural producers make a variety of important decisions before and during the growing season. This support can include: assessing the risk of early and late frosts and freezes that can cause crop damage; comparing corn hybrid maturity requirements and Corn GDD projections to select seed varieties and plan activities such as spraying; guiding marketing decisions based on historical and projected Corn GDDs when considering forward crop pricing (i.e., futures market. The Corn GDD tool provides decision support for corn producers in the central U.S. corn-producing states. Survey results, web statistics, and user feedback indicate that this tool is being actively used by decision makers.

  4. Methyl Anthranilate as a Repellent for Western Corn Rootworm Larvae (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Bernklau, E J; Hibbard, B E; Norton, A P; Bjostad, L B

    2016-08-01

    Methyl anthranilate was identified as the active compound in extracts of maize (Zea mays L.) roots that were shown to be repellent to neonate western corn rootworm (Diabrotica virgifera virgifera LeConte) larvae. A bioassay-driven approach was used to isolate the active material from diethyl ether extracts of roots from germinating maize seeds. Separation of the extract on a Florisil column yielded an active fraction of 90:10 hexane:diethyl ether. Analysis with gas chromatography-mass spectrometry identified two compounds in the active fraction: indole (2,3-benzopyrrole) and methyl anthranilate (methyl 2-aminobenzoate). When tested in behavioral bioassays, methyl anthranilate elicited a significant (P repellent response at doses of 1, 10, and 100 µg. In subsequent single-choice bioassays, 1, 10, and 100 µg of methyl anthranilate prevented larvae from approaching 10 mmol/mol concentrations of carbon dioxide, which is normally highly attractive to the larvae. Indole, the other compound identified from the active fraction, did not elicit a behavioral response by the larvae. Methyl anthranilate has potential for development as a management tool for western corn rootworm larvae and may be best suited for use in a push-pull control strategy.

  5. Exogenous melatonin improves corn (Zea mays L.) embryo proteome in seeds subjected to chilling stress.

    Science.gov (United States)

    Kołodziejczyk, Izabela; Dzitko, Katarzyna; Szewczyk, Rafał; Posmyk, Małgorzata M

    2016-04-01

    Melatonin (MEL; N-acetyl-5-methoxytryptamine) plays an important role in plant stress defense. Various plant species rich in this indoleamine have shown a higher capacity for stress tolerance. Moreover, it has great potential for plant biostimulation, is biodegradable and non-toxic for the environment. All this indicates that our concept of seed enrichment with exogenous MEL is justified. This work concerns the effects of corn (Zea mays L.) seed pre-sowing treatments supplemented with MEL. Non-treated seeds (nt), and those hydroprimed with water (H) or with MEL solutions 50 and 500 μM (HMel50, HMel500) were compared. Positive effects of seed priming are particularly apparent during germination under suboptimal conditions. The impact of MEL applied by priming on seed protein profiles during imbibition/germination at low temperature has not been investigated to date. In order to identify changes in the corn seed proteome after applying hydropriming techniques, purified protein extracts of chilling stressed seed embryos (14 days, 5°C) were separated by two-dimensional electrophoresis. Then proteome maps were graphically and statistically compared and selected protein spots were qualitatively analyzed using mass spectrometry techniques and identified. This study aimed to analyze the priming-induced changes in maize embryo proteome and at identifying priming-associated and MEL-associated proteins in maize seeds subjected to chilling. We attempt to explain how MEL expands plant capacity for stress tolerance.

  6. 玉米蛋白粉的综合利用%Comprehensive utilization of corn gluten meal

    Institute of Scientific and Technical Information of China (English)

    赵文扬; 康明丽; 王蕾娜; 郭小磊

    2014-01-01

    Corn gluten meal is one of the by-products of the processing of corn starch. It contains protein, starch, and maize yellow pigment. Zein is a natural preservative and can be extracted by ethanol or ultrasonic methods. Maize yellow pigment can be extracted by organic solvents, enzymes, and ultrasonic extraction. Corn protein was used to produce bioactive peptides by enzymatic hydrolysis. The bioactive peptide has health function.%玉米蛋白粉是玉米淀粉加工的副产物之一,其中含有蛋白质、淀粉以及玉米黄色素。玉米醇溶蛋白是一种理想的天然保鲜剂,可以采用乙醇或是超声波提取;玉米黄色素可用于食品的着色,通常采用有机溶剂、酶或是超声波提取;玉米蛋白水用酶解制得具有生物活性的肽,具有保健功能。

  7. Environmental effect of conventional and GM crops of cotton (Gossipium hirsitum L. and corn (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Kelly Ávila

    2011-12-01

    Full Text Available In the corn belt of Valle de San Juan and in the cotton zone of El Espinal, municipalities in the department of Tolima (Colombia, 10 conventional corn producers, 10 producers of genetically modified corn, five producers of conventional cotton and 15 producers of transgenic cotton were surveyed in the first half of 2009 to contrast the differences in the environmental impact associated with use of insecticides and herbicides, which were evaluated by estimating the environmental index quotient-EIQ. In the case of maize, an EIQ of 42 was found in the conventional type, while transgenic technology had an EIQ of 3.03. In the cultivation of cotton, an EIQ of 263.59 was found for the conventional type while for transgenic technology this value varied between 335.75 (Nuopal BG/RR and 324.79 (DP 455 BG/RR. These data showed a lower environmental impact using GM technology in the cultivation of maize when compared to the conventional counterpart, in connection with the use of insecticides and herbicides, in the context of time, space and genotypic analysis. This effect was not observed in the case of cotton, where environmental impacts were similar

  8. [Research on maize multispectral image accurate segmentation and chlorophyll index estimation].

    Science.gov (United States)

    Wu, Qian; Sun, Hong; Li, Min-zan; Song, Yuan-yuan; Zhang, Yan-e

    2015-01-01

    In order to rapidly acquire maize growing information in the field, a non-destructive method of maize chlorophyll content index measurement was conducted based on multi-spectral imaging technique and imaging processing technology. The experiment was conducted at Yangling in Shaanxi province of China and the crop was Zheng-dan 958 planted in about 1 000 m X 600 m experiment field. Firstly, a 2-CCD multi-spectral image monitoring system was available to acquire the canopy images. The system was based on a dichroic prism, allowing precise separation of the visible (Blue (B), Green (G), Red (R): 400-700 nm) and near-infrared (NIR, 760-1 000 nm) band. The multispectral images were output as RGB and NIR images via the system vertically fixed to the ground with vertical distance of 2 m and angular field of 50°. SPAD index of each sample was'measured synchronously to show the chlorophyll content index. Secondly, after the image smoothing using adaptive smooth filtering algorithm, the NIR maize image was selected to segment the maize leaves from background, because there was a big difference showed in gray histogram between plant and soil background. The NIR image segmentation algorithm was conducted following steps of preliminary and accuracy segmentation: (1) The results of OTSU image segmentation method and the variable threshold algorithm were discussed. It was revealed that the latter was better one in corn plant and weed segmentation. As a result, the variable threshold algorithm based on local statistics was selected for the preliminary image segmentation. The expansion and corrosion were used to optimize the segmented image. (2) The region labeling algorithm was used to segment corn plants from soil and weed background with an accuracy of 95. 59 %. And then, the multi-spectral image of maize canopy was accurately segmented in R, G and B band separately. Thirdly, the image parameters were abstracted based on the segmented visible and NIR images. The average gray

  9. Utilization of Different Corn Fractions by Broilers

    Directory of Open Access Journals (Sweden)

    SIFR Costa

    2015-09-01

    Full Text Available ABSTRACTThis study was conducted to evaluate the nutritional values of fractions of damaged corn. One hundred and eighty 22-d-old Cobb 500 male broilers were distributed in batteries according to a completely randomized design with six treatments of six replicates each. The treatments consisted of diets containing five corn fractions, classified as sound, fermented, insect-damaged, mold-damaged, or reference corn. The test diets consisted of 60% of reference diet + 40% of each corn fraction. Only the reference corn fraction included all the fractions at different proportions (0.8% fermented, 0.05% insect-damaged, 3.3% mold-damaged, and 95.85% sound grains. The method of total excreta collection was used to determine AMEn values and metabolizability coefficients of dry matter (MDM, crude protein (MCP, ether extract (MEE, and gross energy (MGE of the reference corn and its fractions. The density values of the corn fractions were used to calculate the correlations among the evaluated parameters. The evaluated corn fractions presented different compositions values. The insect-damaged and mold-damaged grains presented higher CP level, lower density, and MDM and MCP coefficients compared with the other fractions. However, calculated AMEn values were not significantly different (p>0.05 among corn fractions. A low correlation between density and AMEn content (r0.8 were calculated. Although the evaluated corn fractions presented different nutritional values, there were no marked differences in their utilization by broilers.

  10. Corning: supplier of multiple optical materials for telescope projects

    Science.gov (United States)

    VanBrocklin, Randy R.; Navan, W. David; Edwards, Mary J.

    2006-06-01

    Corning manufactures several optical materials that can be used as reflective and transmissive optics for telescope optical systems. Corning can manufacture these materials in a large range of sizes and configurations. This paper discusses Corning's portfolio of optical materials and their properties, along with Corning's manufacturing capabilities using these materials. Specific examples of optical blanks that Corning has supplied will be discussed.

  11. 75 FR 48321 - Corning Natural Gas Corporation; Notice of Application

    Science.gov (United States)

    2010-08-10

    ... Energy Regulatory Commission Corning Natural Gas Corporation; Notice of Application August 4, 2010. Take notice that on July 26, 2010, Corning Natural Gas Corporation (Corning), 330 W. William Street, Corning... Natural Gas Act (NGA) requesting the determination of a service area with which Corning may,...

  12. Pest Control in Corn and Soybeans: Weeds - Insects - Diseases.

    Science.gov (United States)

    Doersch, R. E.; And Others

    This document gives the characteristics and application rates for herbicides used to control annual weeds in corn, annual and perennial broadleaf weeds in corn, quackgrass and yellow nutsedge in corn, and annual weeds in soybeans. It also gives insecticide use information for corn and soybeans. A brief discussion of disease control in corn and…

  13. Utilizing protein-lean coproducts from corn containing recombinant pharmaceutical proteins for ethanol production.

    Science.gov (United States)

    Paraman, Ilankovan; Moeller, Lorena; Scott, M Paul; Wang, Kan; Glatz, Charles E; Johnson, Lawrence A

    2010-10-13

    Protein-lean fractions of corn (maize) containing recombinant (r) pharmaceutical proteins were evaluated as a potential feedstock to produce fuel ethanol. The levels of residual r-proteins in the coproduct, distillers dry grains with solubles (DDGS), were determined. Transgenic corn lines containing recombinant green fluorescence protein (r-GFP) and a recombinant subunit vaccine of Escherichia coli enterotoxin (r-LTB), primarily expressed in endosperm, and another two corn lines containing recombinant human collagen (r-CIα1) and r-GFP, primarily expressed in germ, were used as model systems. The kernels were either ground and used for fermentation or dry fractionated to recover germ-rich fractions prior to grinding for fermentation. The finished beers of whole ground kernels and r-protein-spent endosperm solids contained 127-139 and 138-155 g/L ethanol concentrations, respectively. The ethanol levels did not differ among transgenic and normal corn feedstocks, indicating the residual r-proteins did not negatively affect ethanol production. r-Protein extraction and germ removal also did not negatively affect fermentation of the remaining mass. Most r-proteins were inactivated during the mashing process used to prepare corn for fermentation. No functionally active r-GFP or r-LTB proteins were found after fermentation of the r-protein-spent solids; however, a small quantity of residual r-CIα1 was detected in DDGS, indicating that the safety of DDGS produced from transgenic grain for r-protein production needs to be evaluated for each event. Protease treatment during fermentation completely hydrolyzed the residual r-CIα1, and no residual r-proteins were detectable in DDGS.

  14. Utilizing virus-induced gene silencing for the functional characterization of maize genes during infection with the fungal pathogen Ustilago maydis.

    Science.gov (United States)

    van der Linde, Karina; Doehlemann, Gunther

    2013-01-01

    While in dicotyledonous plants virus-induced gene silencing (VIGS) is well established to study plant-pathogen interaction, in monocots only few examples of efficient VIGS have been reported so far. One of the available systems is based on the brome mosaic virus (BMV) which allows gene silencing in different cereals including barley (Hordeum vulgare), wheat (Triticum aestivum), and maize (Zea mays).Infection of maize plants by the corn smut fungus Ustilago maydis leads to the formation of large tumors on stem, leaves, and inflorescences. During this biotrophic interaction, plant defense responses are actively suppressed by the pathogen, and previous transcriptome analyses of infected maize plants showed comprehensive and stage-specific changes in host gene expression during disease progression.To identify maize genes that are functionally involved in the interaction with U. maydis, we adapted a VIGS system based on the Brome mosaic virus (BMV) to maize at conditions that allow successful U. maydis infection of BMV pre-infected maize plants. This setup enables quantification of VIGS and its impact on U. maydis infection using a quantitative real-time PCR (q(RT)-PCR)-based readout.

  15. Research on the status and prospect of selenium-enriched maize%富硒玉米的研究现状及前景展望

    Institute of Scientific and Technical Information of China (English)

    曾艳华; 程伟东; 覃兰秋; 周锦国; 谭贤杰; 江禹奉; 谢和霞; 谢小东

    2015-01-01

    Selenium-enriched maize is a kind of functional maize characterized with high content of seleni-um,fine quality,healthcare,illnessprevention and safety.The research significance of selenium-enriched maize is summarized from three aspects:the effects of selenium on humanhealth,the relationship between selenium and maize growth,and effect of selenium in maize. The research progress of selenium-enriched maize on breeding,cultivation,physiology and biochemistry is overviewed.The direction of further research on selenium rich corn and development prospects in Guangxi are discussed.%富硒功能玉米具有富硒、优质、保健、防病、安全等特点.文章从硒与人体健康、硒与玉米生长发育的关系和玉米的富硒效应三方面概述了富硒玉米的研究意义,综述了富硒玉米在品种选育、生产和生理生化等方面的研究现状及存在的问题,对富硒玉米的进一步研究方向及在广西的发展前景作了展望.

  16. IMPROVING PHOSPHORUS NUTRITION OF CORN

    Directory of Open Access Journals (Sweden)

    Walter B. Gordon

    2014-01-01

    Full Text Available Phosphorus (P generally occurs in soils as the anions H2PO4- or HPO4-2 depending on soil pH. These anions readily react with soil cations such as calcium, magnesium, iron and aluminum to produce various phosphate compounds of very limited water solubility. Crop recovery of applied phosphate fertilizer can be quite low during the season of application. In addition, the large amounts of crop residue present in no-tillage production systems can lower soil temperature thus reducing root growth and nutrient uptake of plants even on soils not low in available Phosphorus (P. Specialty Fertilizer Products, Leawood, KS has developed and patented a product registered as AVAIL® that is reported to attract and sequester antagonistic cations out of the soil solution leaving more of the applied P in available form for plant uptake. The objective of this experiment was to evaluate the effectiveness of AVAIL treated P-fertilizer on growth, P-uptake and yield of irrigated corn (Zea mays L. grown in a no-tillage production system. A 3-year experiment was conducted from 2001-2003 at the North Central Kansas Experiment Field, located near Scandia, KS, on a Crete silt loam soil (fine, montmorillonitic, mesic Pachic Arquistoll. Treatments consisted of three rates of P with or without AVAIL. A no P check plot was also included. When averaged over the years and P rates, the use of AVAIL increased yield of corn by 1.1 Mg ha-1. AVAIL also increased corn dry weight at the six-leaf stage, whole plant P uptake at the six-leaf stage and P concentration at mid-silk. The use of AVAIL proved beneficial in overcoming many of the problems associated with P nutrition in corn. AVAIL consistently increased P uptake and yield in this experiment.

  17. QTL mapping for Mediterranean corn borer resistance in European flint germplasm using recombinant inbred lines

    Directory of Open Access Journals (Sweden)

    Santiago Rogelio

    2010-03-01

    Full Text Available Abstract Background Ostrinia nubilalis (ECB and Sesamia nonagrioides (MCB are two maize stem borers which cause important losses in temperate maize production, but QTL analyses for corn borer resistance were mostly restricted to ECB resistance and maize materials genetically related (mapping populations derived from B73. Therefore, the objective of this work was to identify and characterize QTLs for MCB resistance and agronomic traits in a RILs population derived from European flint inbreds. Results Three QTLs were detected for stalk tunnel length at bins 1.02, 3.05 and 8.05 which explained 7.5% of the RILs genotypic variance. The QTL at bin 3.05 was co-located to a QTL related to plant height and grain humidity and the QTL at bin 8.05 was located near a QTL related to yield. Conclusions Our results, when compared with results from other authors, suggest the presence of genes involved in cell wall biosynthesis or fortification with effects on resistance to different corn borer species and digestibility for dairy cattle. Particularly, we proposed five candidate genes related to cell wall characteristics which could explain the QTL for stalk tunnelling in the region 3.05. However, the small proportion of genotypic variance explained by the QTLs suggest that there are also many other genes of small effect regulating MCB resistance and we conclude that MAS seems not promising for this trait. Two QTLs detected for stalk tunnelling overlap with QTLs for agronomic traits, indicating the presence of pleitropism or linkage between genes affecting resistance and agronomic traits.

  18. The effect of branched limit dextrin on corn and waxy corn gelatinization and retrogradation.

    Science.gov (United States)

    Wang, Lili; Xu, Jin; Fan, Xuerong; Wang, Qiang; Wang, Ping; Yuan, Jiugang; Yu, Yuanyuan; Zhang, Ying; Cui, Li

    2017-08-02

    The effect of branched limit dextrins (BLDs) on the gelatinization and retrogradation properties of corn and waxy corn starch was investigated using differential scanning calorimetry (DSC), wide X-ray diffraction (WXRD). The DSC data showed that the presence of BLDs increased the gelatinization and decreased the gelatinization enthalpy (ΔHgel). The retrogradation of corn and waxy corn starch were retarded by BLDs. The BLD with the lowest molecular weight had the best influence on corn and waxy corn starch retrogradation. The result of WXRD confirmed it. Avrami equation was used to analyze the enthalpies of retrograded corn and waxy corn starch. Starch recrystallization rate (k) reduced with the addition of BLDs, indicating that BLDs reduced the kinetics of starch retrogradation. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Effect of transgenic corn hybrids and a soil insecticide on corn rootworm (Coleoptera: Chrysomelidae) beetle emergence in North Dakota

    Science.gov (United States)

    Northern, Diabrotica barberi Smith & Lawrence, and western corn rootworms, D. virgifera virgifera LeConte, are economic pests of corn, Zea mays L. (Poaceae) in North Dakota. Many area corn growers rely on transgenic Bt (Bacillus thuringiensis) corn hybrids to manage corn rootworms. Our objective was...

  20. Starch Accumulation and Enzyme Activities Associated with Starch Synthesis in Maize Kernels

    Institute of Scientific and Technical Information of China (English)

    ZHANG Hai-yan; DONG Shu-ting; GAO Rong-qi; SUN Qing-quan

    2007-01-01

    The filling rate and the starch accumulation in developing maize kernel were analyzed. The changes of enzyme activities associated with sucrose metabolism and starch biosynthesis were investigated. The purpose is to discuss the enzymatic mechanisms responsible for starch synthesis. Two types of maize cultivars (Zea mays), high starch maize (Feiyu 3) and normal maize (Yuyu 22), were grown in a corn field. The factors involved in starch synthesis were performed during the growth period. The kernel filling rate, the sucrose content, the starch accumulating rates and the activities of SS (sucrose synthase), GBSS (granule-bound starch synthase), SBE (starch branching enzyme) of Feiyu 3, which has high starch content, were significantly higher than those of Yuyu 22, which has low starch content, after 10 DAP (days after pollination).Correlation analysis indicated that ADPGPPase (ADP-glucose pyrophosphorylase) and DBE (starch debranching enzyme)were not correlated with the starch accumulating rates and the kernel filling rate, but the SS activity at the middle and late period were highly significantly correlated with the starch accumulating rates and the kernel filling rate. The GBSS activity was highly significantly correlated with the amylose accumulating rate, but not correlated with the kernel filling rate. The SBE activity was highly significantly correlated with the amylopectin accumulating rate and the kernel filling rate. It was not ADPGPPase and DBE, but SS was the rate-limiting factor of starch biosynthesis in developing maize kernels. GBSS had an important effect on amylose accumulation, and SBE had a significant effect on amylopectin accumulation.

  1. Maize starch biphasic pasting curves

    CSIR Research Space (South Africa)

    Nelles, EM

    2000-05-01

    Full Text Available (150–500 rev/min). The second pasting peak is attributed to the formation of complexes between amylose and low levels of lipid present in maize starch. When lipid was partially removed by extraction with methanol-chloroform (1: 3 v/v), the second...

  2. Production of ethyl alcohol from corn silage

    Energy Technology Data Exchange (ETDEWEB)

    Pieper, H.J.; Ponitz, H.

    1973-01-01

    Corn silage may be employed as a raw material for the production of ethyl alcohol when starch is first cracked by pressure cooking and subsequently saccharified by microbial amalyses. Cracking conditions are: pressure increase 1.6 atmosphere within 60 minutes; maximum maintained for 35 minutes. The fermentation is complete after 72 hours. Extract decreases of fermented mashes made from corn silage are less than when dried corn is used. In the most advantageous case the degree of fermentation was -0.2 weight % of the extract. The maximum yields of alcohol were 26.0.1. pure alcohol/100 kg corn silage and 61.2.1. pure alcohol/100 kg starch. The latter is 3.9.1. pure alcohol lower than when dried corn was used. Despite the high bacterial infection of corn silage practically infection-free processing is assured.

  3. Climate change and corn susceptibility to mycotoxins

    Science.gov (United States)

    Maize is an essential part of the world’s grain supply, but climate change has the potential to increase maize susceptibility to mycotoxigenic fungal pathogens and reduce food security and safety. While rising atmospheric [CO2] is a driving force of climate change, our understanding of how elevated ...

  4. The potential of residues of furfural and biogas as calcareous soil amendments for corn seed production.

    Science.gov (United States)

    Zhao, Yunchen; Yan, Zhibin; Qin, Jiahai; Ma, Zhijun; Zhang, Youfu; Zhang, Li

    2016-04-01

    Intensive corn seed production in Northwest of China produced large amounts of furfural residues, which represents higher treatment cost and environmental issue. The broad calcareous soils in the Northwest of China exhibit low organic matter content and high pH, which led to lower fertility and lower productivity. Recycling furfural residues as soil organic and nutrient amendment might be a promising agricultural practice to calcareous soils. A 3-year field study was conducted to evaluate the effects of furfural as a soil amendment on corn seed production on calcareous soil with compared to biogas residues. Soil physical-chemical properties, soil enzyme activities, and soil heavy metal concentrations were assessed in the last year after the last application. Corn yield was determined in each year. Furfural residue amendments significantly decreased soil pH and soil bulk density. Furfural residues combined with commercial fertilizers resulted in the greater cumulative on soil organic matter, total phosphorus, available phosphorus, available potassium, and cation exchange capacity than that of biogas residue. Simultaneously, urease, invertase, catalase, and alkaline phosphatase increased even at the higher furfural application rates. Maize seed yield increased even with lower furfural residue application rates. Furfural residues resulted in lower Zn concentration and higher Cd concentration than that of biogas residues. Amendment of furfural residues led to higher soil electrical conductivity (EC) than that of biogas residues. The addition of furfural residues to maize seed production may be considered to be a good strategy for recycling the waste, converting it into a potential resource as organic amendment in arid and semi-arid calcareous soils, and may help to reduce the use of mineral chemical fertilizers in these soils. However, the impact of its application on soil health needs to be established in long-term basis.

  5. Combing Ability Analysis ofamong Early Generation Maize Inbred ...

    African Journals Online (AJOL)

    dagne.cimdom

    Combining ability estimates are important genetic attributes ina maize breeding ... Twenty-nine early generation maize inbred lines were crossed to two ...... agronomic traits in quality protein maize under stress and non stress environments.

  6. 21 CFR 184.1857 - Corn sugar.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Corn sugar. 184.1857 Section 184.1857 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN... Substances Affirmed as GRAS § 184.1857 Corn sugar. (a) Corn sugar (C6H12O6, CAS Reg. No. 50-99-7), commonly...

  7. Cassava and corn starch in maltodextrin production

    OpenAIRE

    Geovana Rocha Plácido Moore; Luciana Rodrigues do Canto; Edna Regina Amante; Valdir Soldi

    2005-01-01

    Maltodextrin was produced from cassava and corn starch by enzymatic hydrolysis with alpha-amylase. The cassava starch hydrolysis rate was higher than that of corn starches in maltodextrin production with shorter dextrose equivalent (DE). DE values do not show directly the nature of the obtained oligosaccharides. Maltodextrin produced from cassava and corn starch was analysed by high performance liquid chromatography (HPLC), and the analysis showed that maltodextrin production differs accordin...

  8. 新疆玉米生产及机械化技术发展探讨%Production and Mechanization Technology Development Situation of the Corn in Xinjiang

    Institute of Scientific and Technical Information of China (English)

    张静; 韩长杰; 郭辉; 杨宛章

    2014-01-01

    Through the analysis of the corn production and mechanization development situation in Xinjiang, the paper summaries the main problems existing in corn mechanization, and proposed the measures for maize production mechanization, it has very important significance to improve the level of mechanization of corn production.%通过对新疆玉米生产现状及机械化发展概况的分析,概述新疆玉米机械化存在的主要问题,提出适合玉米机械化生产的对策,对提高玉米生产机械化水平具有十分重要的意义。

  9. Study of Techno-Economic of Corn Sheller Type MPJ-01-TEP-2014 Design of Study Program Agricultural Engineering Program, University of Andalas

    Directory of Open Access Journals (Sweden)

    - Mislaini

    2015-02-01

    Full Text Available This study aims to evaluate the performance of the machine corn sheller and perform basic cost analysis and break-even point. Sheller was done on three varieties of maize in Padang and surrounding areas. Corn used is corn that has a moisture content (17-20% with three repetitions. Based on research, capacity sheller machine Maxpro NK 99 varieties obtained 80.49 kg / h with the cost of Rp.120,88 / kg and breakeven 38135.59 kg / year. Pioner 24 varieties of working capacity of 67.52 kg / h with the basic cost of Rp 114.48 / kg and the breakeven point at 66399.66 kg / year, and the work capacity of NK Maxpro 22 varieties of 55.40 kg / h with a cost of Rp. 139.53 / kg with a breakeven point 40186.26 kg / year.

  10. Proteins induced in corn (Zea mays) in response to the European corn borer (Ostrinia nubilalis)

    Science.gov (United States)

    The European corn borer (ECB, Ostrinia nubilalis) is a major pest of corn. ECB begin by feeding in the whorl tissue and then eventually tunnel into the stalk of the corn where they cause most of the damage. Tunneling can disrupt the transport of water and nutrients in the plant and it provides sites...

  11. Understanding successful resistance management: The European corn borer and Bt corn in the United States

    Science.gov (United States)

    European corn borer, Ostrinia nubilalis Hubner (Lepidoptera: Crambidae) has been a major pest of corn and other crops in North America since its accidental introduction nearly a hundred years ago. Wide adoption of transgenic corn that expresses toxins from Bacillus thuringiensis, referred to as Bt c...

  12. Research on mechanical properties of corn stalk

    Science.gov (United States)

    Zhang, Kaifei; He, Yujing; Zhang, Hongmei; Li, He

    2017-03-01

    Many domestic scholars have studied on straw utilization from lodging resistance, by breeding agricultural experts to optimization parameters, which selected by agricultural mechanical experts and efficient utilization after the harvest crush. Therefore, the study of the mechanical properties of corn stalks has great prospects. It can provide the basis for the design of agricultural machinery and comprehensive utilization of straw that study the relationship between the properties of the corn stalk and the mechanical properties. In this paper, the radial compression and bending mechanical properties of corn stalk was conducted by universal material testing machine, which contributes to the increase of corn crop and provides basis for the development of equipment.

  13. 我国玉米收获机械化发展剖析%Analysis of the Development of Mechanized Maize Harvesting in China

    Institute of Scientific and Technical Information of China (English)

    班春华

    2013-01-01

    This article gives a brief introduction of the development of mechanized maize production, makes analysis of the development status of mechanized maize harvesting abroad, examines the main reasons for the backward level of China's corn harvest mechanization, and puts forward corresponding suggestions so as to provide reference for improving China's mechanization in maize harvesting.%在简要介绍发展玉米生产全程机械化的重要意义基础上,分析国外玉米收获机械化的发展现状,探讨我国玉米收获机械化水平落后的主要原因,并提出相应的发展建议,为提高我国玉米收获机械化的水平提供参考。

  14. Assessment of maize stem borer damage on hybrid maize varieties in Chitwan, Nepal

    OpenAIRE

    Buddhi Bahadur Achhami; Santa Bahadur BK; Ghana Shyam Bhandari

    2015-01-01

    Maize is the second most important cereal crop in Nepal. However, national figure of grain production still remains below than the world's average grain production per unit area. Thus, this experiment was designed to determine the suitable time of maize planting, and to assess the peak period of one of the major insects, maize stem borer, in Chitwan condition. The results showed that plant damage percentage as per the maize planting month varies significantly, and the average plant damage per...

  15. Assessment of maize stem borer damage on hybrid maize varieties in Chitwan, Nepal

    OpenAIRE

    Buddhi Bahadur Achhami; Santa Bahadur BK; Ghana Shyam Bhandari

    2015-01-01

    Maize is the second most important cereal crop in Nepal. However, national figure of grain production still remains below than the world's average grain production per unit area. Thus, this experiment was designed to determine the suitable time of maize planting, and to assess the peak period of one of the major insects, maize stem borer, in Chitwan condition. The results showed that plant damage percentage as per the maize planting month varies significantly, and the average plant damage...

  16. Effect of winter maize-based intercropping systems on maize yield, associated weeds and economic efficiency

    OpenAIRE

    2014-01-01

    A field experiment was conducted during winter seasons of 2003-04 and 2004-05 at Kanpur, India to study the effect of winter maize (Zea mays L.) based intercropping systems on maize yield, associated weeds and economics under irrigated condition of central Uttar Pradesh. Thirteen maize-based cropping systems such as maize sole, potato (Solanum tuberosum L.) sole, mustard [Brassica juncea (L.) Czernj. & Cosson] sole, toria (Brassica campestris var. toria) sole, pea (Pisum sativum L.) sole, lin...

  17. GROWTH AND PRODUCTIVITY OF SPECIALITY CORN AS INFLUENCED BY DIFFERENT LEVELS OF NITROGEN UNDER PONGAMIA PLANTATIONS

    Directory of Open Access Journals (Sweden)

    C. Prathyusha

    2013-12-01

    Full Text Available A field experiment was conducted during kharif 2011 at the Student’s Farm, College of Agriculture, Rajendranagar, Hyderabad on red sandy loam soils to study the effect of nitrogen management in speciality corn under Pongamia + maize agri-silvi system. All the growth and yield attributes such as plant height, dry matter production, leaf area index, cob length, cob girth, number of cobs plant-1, number of rows cob-1, number of kernals cob-1 and 100 kernel weight were found maximum at 120 kg N ha-1 than at the remaining nitrogen levels. Whereas, cob weight (with husk was found maximum at 120 kg ha-1 but was on par with 90 kg N ha-1. Similarly cob yield (with husk, green fodder/stover yield, harvest index, kernel yield of popcorn and shelling percentage of popcorn were found significantly higher at 120 kg N ha-1 than the other two lower doses of nitrogen. The different types of corn were found significantly different from each other regarding growth parameters such as plant height, days to 50 per cent silking and days to maturity. Regarding the effect on yield attributes and yield, all the three types of corn were found significantly different from each other in cob length, cob girth, cob weight (with husk, green cob yield as well as green fodder/stover yield

  18. MaizeGDB: Global support for maize research through open access information [abstract

    Science.gov (United States)

    MaizeGDB is the open-access global repository for maize genetic and genomic information – from single genes that determine nutritional quality to whole genome-scale data for complex traits including yield and drought tolerance. The data and tools at MaizeGDB enable researchers from Ethiopia to Ghan...

  19. Long-term Effects of Early Life Maize Yield on Maize Productivity and Efficiency in Rural Malawi

    OpenAIRE

    2017-01-01

    The paper assesses the effects of maize yields just prior to birth (in utero), in the first and the second years of life on adult life productivity and efficiency of maize farmers born between 1984 and 1995 in rural Malawi. To ensure that early life maize yields are not confounded by omitted local chacteristics, they are transformed into relative maize yields by using a cumulative gamma distribution. I find that maize yield just prior to birth significantly increases maize output in a farmer'...

  20. Application of csm- ceres-maize model for seasonal and multi-decadal predictions of maize yield in under subtropical condition of Chitwan, Nepal

    Directory of Open Access Journals (Sweden)

    Lal Prasad Amgain

    2015-12-01

    Full Text Available The average maize yield of 2.5 t/ha in sub-tropical terai and inner terai of Nepal has been very less than its potential yield of about 5.0 t/ha, for which changing climatic scenarios have been reported the critical factors. Cropping system Model (CSM-Crop Estimation through Resource and Environment Synthesis (CERES-Maize, embedded under Decision Support System for Agro-technology Transfer (DSSAT ver. 4.2 was evaluated from a datasets of field experimentation by growing four diverse maize genotypes viz. full season OPV (Rampur Composite, Quality Protein Maize (Posilo Makai-1, Hybrid (Gaurav and Pop corn (Pool-12 under three different planting dates (September 1, October 1 and November 1 in 2009-10 at Rampur Campus, Chitwan. The experiment was laid out in two factor factorial randomized complete block design (RCBD with three replications in slightly acidic (pH 6.7 sandy loam soil having low soil available N( 0.49% and K (148 kg/ha and medium P (16.3 kg/ha status. The ancillary and yield data obtaining from field experiment was analyzed from the M-Stat C software and recorded that Gaurav hybrid produced significantly higher yield under September 1 planting (5.86 t/ha followed by Posilo Makai 1 (5.55 t/ha, Rampur Composite (5.1t/ha and the least with Pool-12 (3.45 t/ha. Further, the heat use efficiency of diverse maize genotypes were also calculated by using the mean temperature based accumulative heat unit system and found the stable yields only with Rampur Composite for all planting dates and the rest genotypes were suitable only to the early winter plantings. Model calibration was done by using September 1 planting date for all 4 maize genotypes while validation was accomplished by using the remaining treatments for predicting growth and yield of different maize genotypes. The year 2006- 07 was found 13, 18, 23 and 7% higher in producing the maize yield than the standard year 2009-10 for Rampur Composite, Posilo Makai-1, Gaurav and Pool-12

  1. Nutrition Characters of Sweet Corns in Kernel Milky Maturity

    Institute of Scientific and Technical Information of China (English)

    WuMoucheng; ChenXiaoyi

    2000-01-01

    Three corn varieties,supper-sweet corn(S),standard-sweet corn(M),corn-non corn(C) were used for nutritional composition measurement during kernel milky maturity.The variations of protein,vitamin,total sugar showed as parabola.Mineral elements and fiber increase and reducing sugar decreased gradually.Total sugar,protein and fat in the sweet corn were much richer than those in common corn.VE and VC were very plentiful,and lysine was high.Proper harvest time of sweet corn M and S were DAP (days after pollination)19-21,and DAP 18-21 respectively.

  2. 21 CFR 73.315 - Corn endosperm oil.

    Science.gov (United States)

    2010-04-01

    ... corn endosperm oil is a reddish-brown liquid composed chiefly of glycerides, fatty acids, sitosterols.... (b) Specifications. Corn endosperm oil conforms to the following specifications: Total fatty...

  3. Expression of a Modified Crylle Gene in E.Coli and in Transgenic Tobacco Confers Resistance to Corn Borer

    Institute of Scientific and Technical Information of China (English)

    Yun-Jun LIU; Fu-Ping SONG; Kang-Lai HE; Yuan YUAN; Xiao-Xia ZHANG; Peng GAO; Jian-Hua WANG; Guo-Ying WANG

    2004-01-01

    The wild-type Crylle gene from Bacillus thuringiensis was modified for its efficient expression in transgenic plants. Modified Cry1 Ie gene (designated as Cry1 Iem) was cloned into prokaryotic expression vector pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays using crude expression products in E. coli revealed that Cry1 Iem protein had a similar toxicity to corn borer as wild-type Cry1 Ie. Cry1 Iem gene was then inserted downstream of the maize ubiquitin-1 promoter in plant expression vector p3301. Transgenic tobacco plants carrying Cry1 Iem showed insecticidal activity against com borer.

  4. "Achieving Mexico’s Maize Potential"

    OpenAIRE

    Antonio Turrent Fernández; Timothy A. Wise; Elise Garvey

    2012-01-01

    Rising agricultural prices, combined with growing import dependence, have driven Mexico’s food import bill over $20 billion per year and increased its agricultural trade deficit. Mexico imports one-third of its maize, overwhelmingly from the United States, but three million producers grow most of the country’s white maize, which is used primarily for tortillas and many other pluricultural products for human consumption. Yield gaps are large among the country’s small to medium-scale maize farm...

  5. Presence of deoxynivalenol in maize of Vojvodina

    OpenAIRE

    Jajić Igor M.; Abramović Biljana F.; Jurić Verica B.; Krstović Saša Z.

    2007-01-01

    By applying previously established optimal conditions for the determination of deoxynivalenol (DON) by liquid chromatography with DAD detector, in this work, its content was determined in maize samples collected during the past 3 years (2004-2006) from different locations in Vojvodina. Analyzing 103 maize samples in total, the presence of deoxynivalenol was established in 42.7% of the samples. Only 3 samples of maize contained DON in concentrations that exceeded the maximum permitted level (1...

  6. Field effects of cadmium contamination in the radiation characteristics of maize

    Science.gov (United States)

    Illes, B.; Anda, A.

    2012-04-01

    Cadmium is one of the most common toxic heavy metals in our environment. Cadmium is a particularly dangerous element, because it dissolves readily, making it easily available to plants. It is thus able to accumulate in various links in the food chain, finally reaching humans, at the end of the chain. Adverse effects on human body was reported in 1858 at first. If it enters the body, damage to health, cause changes and can also cause cancer. Our study was designated to simulate the effects of cadmium on maize in field conditions, during the 2011 growing season. The impact of cadmium on maize was investigated at the Agro-meteorological Research Station in Keszthely. A Swiss-bred maize hybrid, Sperlona (FAO 340), with a short vegetation period, was sown in the experiments at the plant density (70,000 plants per hectare) widely used under Hungarian climatic conditions for growing grain maize. Effects of cadmium on corn life were studied under two water supplies. Evapotranspirometers of the Thornthwaite type were used for the "ad libitum" treatment and the the rainfed variant was sown in field plots. 0,5 M concentration of cadmium was used, which was sprayed weekly. The aim of the investigation was to simulate impact of atmospheric pollution of traffic origin (low and frequent doses in the field). Plant height was registered weekly similarly to leaf area index (LAI). Albedo was measured by pyranometers of the CMA-11 type (Kipp & Zonen, Vaisala). From this the most important radiative properties were calculated, so the net radiation balance, latent heat, sensible heat and the Bowen ratio. The values of LAI for the cadmium contaminated maize were significantly lower compared to the control maize. The net radiation balance was about the same in both treatments. Cadmium causes the latent heat decreased, while the sensible heat increased compared to the control treatment. The Bowen ratio in the polluted crops was higher, than the cadmium-untreated area. The yield of maize

  7. Maize genome sequencing by methylation filtration.

    Science.gov (United States)

    Palmer, Lance E; Rabinowicz, Pablo D; O'Shaughnessy, Andrew L; Balija, Vivekanand S; Nascimento, Lidia U; Dike, Sujit; de la Bastide, Melissa; Martienssen, Robert A; McCombie, W Richard

    2003-12-19

    Gene enrichment strategies offer an alternative to sequencing large and repetitive genomes such as that of maize. We report the generation and analysis of nearly 100,000 undermethylated (or methylation filtration) maize sequences. Comparison with the rice genome reveals that methylation filtration results in a more comprehensive representation of maize genes than those that result from expressed sequence tags or transposon insertion sites sequences. About 7% of the repetitive DNA is unmethylated and thus selected in our libraries, but potentially active transposons and unmethylated organelle genomes can be identified. Reverse transcription polymerase chain reaction can be used to finish the maize transcriptome.

  8. A Comparison of the Effects of Three GM Corn Varieties on Mammalian Health

    Directory of Open Access Journals (Sweden)

    Joël Spiroux de Vendômois, François Roullier, Dominique Cellier, Gilles-Eric Séralini

    2009-01-01

    Full Text Available We present for the first time a comparative analysis of blood and organ system data from trials with rats fed three main commercialized genetically modified (GM maize (NK 603, MON 810, MON 863, which are present in food and feed in the world. NK 603 has been modified to be tolerant to the broad spectrum herbicide Roundup and thus contains residues of this formulation. MON 810 and MON 863 are engineered to synthesize two different Bt toxins used as insecticides. Approximately 60 different biochemical parameters were classified per organ and measured in serum and urine after 5 and 14 weeks of feeding. GM maize-fed rats were compared first to their respective isogenic or parental non-GM equivalent control groups. This was followed by comparison to six reference groups, which had consumed various other non-GM maize varieties. We applied nonparametric methods, including multiple pairwise comparisons with a False Discovery Rate approach. Principal Component Analysis allowed the investigation of scattering of different factors (sex, weeks of feeding, diet, dose and group. Our analysis clearly reveals for the 3 GMOs new side effects linked with GM maize consumption, which were sex- and often dose-dependent. Effects were mostly associated with the kidney and liver, the dietary detoxifying organs, although different between the 3 GMOs. Other effects were also noticed in the heart, adrenal glands, spleen and haematopoietic system. We conclude that these data highlight signs of hepatorenal toxicity, possibly due to the new pesticides specific to each GM corn. In addition, unintended direct or indirect metabolic consequences of the genetic modification cannot be excluded.

  9. RESPONSE OF THE JAMAICA-BEAN-CORN POLYCULTURE CROPPING SYSTEM TO THREE TREATMENTS OF FERTILIZATION IN VILLAFLORES, CHIAPAS, MEXICO

    Directory of Open Access Journals (Sweden)

    Rosey Obet Ruiz-González

    2014-07-01

    Full Text Available The corn (Zeamays Linneo and bean (Phaseolusvulgaris Linneo are the economic basis to meet the basic needs of rural families, but these crops are no longer economically viable for various reasons and jeopardize the family livelihood and productive alternatives are needed to improve the rural economy. The research was conducted during the spring-summer cycle from 1999 to 2000 in Villaflores , Chiapas, in order to evaluate the response of polyculturejamaica-bean-corn three fertilization treatments ( 00-00-00 , 60-60-60 and 120-60-60 . The experimental design was random blocks with three fertilization treatments, seven patterns of crops, and three replications and performance in the three plant species and vegetative variables were evaluated. With the data an ANOVA was performed and the treatment means were compared with the test of Tukey (p ≤ 0.05. The best vegetative response of the jamaica was to associate it with beans and corn with 60-60 fertilization treatment - 60, getting as much of: foliage (170 hojasplanta-1, branches (31 ramasplanta-1, acorns (54 bellotasplanta-1 and increased performance of dried calyxes (698 kgha-1.The beans associated with maize without fertilizations showed increase in foliage (62 hojasplanta-1 and in monoculture with fertilization 60-60 - 60 had the best performance (1,565 kgha-1of beans. Corn had the best vegetative response (18 hojasplanta-1 associated with beans and fertilization 120 - 60-60, and the best performance was when teamed with the beans and the jamaica (7,667 kgha-1. The best equivalent land-use was 3.31 in jamaica-bean-corn crop pattern. The analysis of the results indicates that the jamaica by associating it with corn and beans represents a biological and economic production alternative to improve the economy of rural families in Mexican soils which are acidified.

  10. Periodic Physical Disturbance: An Alternative Method for Controlling Sitophilus zeamais (Maize Weevil Infestation

    Directory of Open Access Journals (Sweden)

    Rashid Suleiman

    2016-09-01

    Full Text Available Sitophilus zeamais Motschulsky is the most important insect pest of stored maize in tropical regions. The objective of this study was to determine the practicality of periodic physical disturbance on S. zeamais mortality and its adoption by smallholder farmers in developing countries. In this experiment, treatments and control were arranged in a randomized block design with three replications and three storage times in three regions of Tanzania. Region was used as the blocking variable. A total of 108 clean 20-L plastic containers were each loaded with 10 kg of fresh white dent corn and 0.50 kg of maize infested with S. zeamais. For the treatment, containers were disturbed twice a day, whereas for the controls the containers were not disturbed until the end of storage. The overall mortality rate (% after 30, 60, and 90 days of storage were 88%, 96%, and 98%, respectively. A statistically significant difference (p < 0.05 was observed for the number of live S. zeamais between the control and experimental treatments. Additionally, the number of live S. zeamais in the treatment significantly decreased as storage time increased. This study shows the potential of a feasible, simple, affordable, and effective method of protecting maize grain for small-holder farmers in developing countries without using chemicals.

  11. Testing the link between genome size and growth rate in maize

    Directory of Open Access Journals (Sweden)

    Maud I. Tenaillon

    2016-09-01

    Full Text Available Little is known about the factors driving within species Genome Size (GS variation. GS may be shaped indirectly by natural selection on development and adaptative traits. Because GS variation is particularly pronounced in maize, we have sampled 83 maize inbred lines from three well described genetic groups adapted to contrasted climate conditions: inbreds of tropical origin, Flint inbreds grown in temperate climates, and Dent inbreds distributed in the Corn Belt. As a proxy for growth rate, we measured the Leaf Elongation Rate maximum during nighttime (LERmax as well as GS in all inbred lines. In addition we combined available and new nucleotide polymorphism data at 29,090 sites to characterize the genetic structure of our panel. We found significant variation for both LERmax and GS among groups defined by our genetic structuring. Tropicals displayed larger GS than Flints while Dents exhibited intermediate values. LERmax followed the opposite trend with greater growth rate in Flints than in Tropicals. In other words, LERmax and GS exhibited a significantly negative correlation (r = − 0.27. However, this correlation was driven by among-group variation rather than within-group variation—it was no longer significant after controlling for structure and kinship among inbreds. Our results indicate that selection on GS may have accompanied ancient maize diffusion from its center of origin, with large DNA content excluded from temperate areas. Whether GS has been targeted by more intense selection during modern breeding within groups remains an open question.

  12. Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiao-li; SI Bing-wen; FAN Cheng-ming; LI Hong-jie; WANG Xiao-ming

    2014-01-01

    Northern corn leaf blight (NCLB), caused by the heterothallic ascomycete fungus Setosphaeria turcica, is a destructive foliar disease of maize and represents a serious threat to maize production worldwide. A comparative proteomic study was conducted to explore the molecular mechanisms underlying the defense responses of the maize resistant line A619 Ht2 to S. turcica race 13. Leaf proteins were extracted from mock and S. turcica-infected leaves after inoculated for 72 h and analyzed for differentially expressed proteins using two-dimensional electrophoresis and mass spectrometry identiifcation. 137 proteins showed reproducible differences in abundance by more than 2-fold at least, including 50 up-regulated proteins and 87 down-regulated proteins. 48 protein spots were successfully identiifed by MS analysis, which included 10 unique, 6 up-regulated, 20 down-regulated and 12 disappeared protein spots. These identiifed proteins were classiifed into 9 functional groups and involved in multiple functions, particularly in energy metabolism (46%), protein destination and storage (12%), and disease defense (18%). Some defense-related proteins were upregulated such asβ-glucosidase, SOD, polyamines oxidase, HSC 70 and PPIases; while the expressions of photosynthesis- and metabolism-related proteins were down-regulated, by inoculation with S. turcica. The results indicated that a complex regulatory network was functioned in interaction between the resistant line A619 Ht2 and S. turcica. The resistance processes of A619 Ht2 mainly resided on directly releasing defense proteins, modulation of primary metabolism, affecting photosyntesis and carbohydrate metabolism.

  13. Metabolic and Phenotypic Responses of Greenhouse-Grown Maize Hybrids to Experimentally Controlled Drought Stress

    Institute of Scientific and Technical Information of China (English)

    Sandra Witt; Luis Galici; Jan Lisec; Jill Cairns; Axel Tiessen; Jose Luis Araus; Natalia Palacios-Rojas; Alisdair R.Fernie

    2012-01-01

    Adaptation to abiotic stresses like drought is an important acquirement of agriculturally relevant crops like maize.Development of enhanced drought tolerance in crops grown in climatic zones where drought is a very dominant stress factor therefore plays an essential role in plant breeding.Previous studies demonstrated that corn yield potential and enhanced stress tolerance are associated traits.In this study,we analyzed six different maize hybrids for their ability to deal with drought stress in a greenhouse experiment.We were able to combine data from morphophysiological parameters measured under well-watered conditions and under water restriction with metabolic data from different organs.These different organs possessed distinct metabolite compositions,with the leaf blade displaying the most considerable metabolome changes following water deficiency.Whilst we could show a general increase in metabolite levels under drought stress,including changes in amino acids,sugars,sugar alcohols,and intermediates of the TCA cycle,these changes were not differential between maize hybrids that had previously been designated based on field trial data as either drought-tolerant or susceptible.The fact that data described here resulted from a greenhouse experiment with rather different growth conditions compared to natural ones in the field may explain why tolerance groups could not be confirmed in this study.We were,however,able to highlight several metabolites that displayed conserved responses to drought as well as metabolites whose levels correlated well with certain physiological traits.

  14. Contexts of offerings and ritual maize in the pictographic record in Central Mexico

    Directory of Open Access Journals (Sweden)

    Natalia Moragas Segura

    2015-01-01

    Full Text Available The objective of this article is an initial enquiry into the evidence and classification of the offerings of maize in Central Mexico from the Classic period to early colonial times. In order to achieve this goal, we will analyse the presence of maize in Central Mexico according to the evidence found in mural paintings and some pictographic codices. Two Mesoamerican cultures will be considered to achieve our analysis: the Teotihuacan and Mexico-Tenochtitlan. Maize was instrumental in the performance of daily rituals and in the diet of these ancient Mesoamerican cultures and the cereal also had sacred connotations in pre-Hispanic, colonial and contemporary narratives. We suggest this by reading the iconographic and symbolic representations of corn in the form of seeds and pods, or as an ingredient in cooked foods which are represented in the mural paintings of Teotihuacan as well as some codices of the post-Classic Nahua tradition. These methodological enquiries reveal evidence of a cultural continuity in Central Mexico as a contrasting perspective on the archaeological and ethno-historical period.

  15. Transcriptome analysis of maize leaf systemic symptom infected by Bipolaris zeicola.

    Science.gov (United States)

    Liu, Ming; Gao, Jian; Yin, Fuqiang; Gong, Guoshu; Qin, Cheng; Ye, Kunhao; Zhang, Min; Sun, Xiaofang; Zhou, You; Zhang, Youju

    2015-01-01

    Bipolaris zeicola is a fungal pathogen that causes Northern corn leaf spot (NCLS), which is a serious foliar disease in maize and one of the most significant pathogens affecting global food security. Here, we report a genome-wide transcriptional profile analysis using next-generation sequencing (NGS) of maize leaf development after inoculation with B. zeicola. We performed High-Throughput Digital Gene Expression analysis to identify differentially expressed genes (DEGs) in resistant inbred Mo17 lines after infection with B. zeicola at four successive disease development stages--CP (contact period), PP (penetration period), IP (incubation period), and DP (disease period); the expression of the genes was compared with those in a CK (mock-treatment) control. In addition, a sensitive maize line (Zheng58) was used for the comparisons with the Mo17. Among all tested genes, 466 differentially expressed genes were identified in all libraries, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these genes suggested that they are involved in many biological processes related to systemic symptom development, such as plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis and photosynthesis. Our systematic analysis provides comprehensive transcriptomic information regarding systemic symptom development in fungal-infected plants. This information will help in furthering our understanding of the detailed mechanisms of plant responses to fungal infection.

  16. Transcriptome analysis of maize leaf systemic symptom infected by Bipolaris zeicola.

    Directory of Open Access Journals (Sweden)

    Ming Liu

    Full Text Available Bipolaris zeicola is a fungal pathogen that causes Northern corn leaf spot (NCLS, which is a serious foliar disease in maize and one of the most significant pathogens affecting global food security. Here, we report a genome-wide transcriptional profile analysis using next-generation sequencing (NGS of maize leaf development after inoculation with B. zeicola. We performed High-Throughput Digital Gene Expression analysis to identify differentially expressed genes (DEGs in resistant inbred Mo17 lines after infection with B. zeicola at four successive disease development stages--CP (contact period, PP (penetration period, IP (incubation period, and DP (disease period; the expression of the genes was compared with those in a CK (mock-treatment control. In addition, a sensitive maize line (Zheng58 was used for the comparisons with the Mo17. Among all tested genes, 466 differentially expressed genes were identified in all libraries, and Kyoto Encyclopedia of Genes and Genomes (KEGG pathway analysis of these genes suggested that they are involved in many biological processes related to systemic symptom development, such as plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis and photosynthesis. Our systematic analysis provides comprehensive transcriptomic information regarding systemic symptom development in fungal-infected plants. This information will help in furthering our understanding of the detailed mechanisms of plant responses to fungal infection.

  17. Climate forecasts for corn producer decision making

    Science.gov (United States)

    Corn is the most widely grown crop in the Americas, with annual production in the United States of approximately 332 million metric tons. Improved climate forecasts, together with climate-related decision tools for corn producers based on these improved forecasts, could substantially reduce uncertai...

  18. 甜玉米%Growing Good Corn

    Institute of Scientific and Technical Information of China (English)

    李同良

    2007-01-01

    @@ James Bender, in his book How to Talk Well (New York: McGraw-Hill Book Company, Inc., 1994)relates the story of a farmer who grew awardwinning corn. Each year he entered his corn in the state fair where it won a blue ribbon. One year a newspaper reporter interviewed him and learned something interesting about how he grew it.

  19. Corn stalk as a bioenergy resource

    Science.gov (United States)

    Haney, Paul E., Jr.

    Waste corn stalk has the potential to help reduce the nation's dependence upon foreign sources of petroleum by becoming a major bioenergy resource. There are many sources of biomass that could also be utilized for this endeavor. It is estimated that over 100 million tons of agricultural waste are produced in the United States alone. This represents a significant source of energy. Through gasification, this waste could be used to generate power, fuels, and/or products. This dissertation shows that the gasification of corn stalk can produce char, heat, synthesis gases (CO and H2), and can also be used for work to dry moist biomass. Through the integration of drying, gasification, and carbon production, waste corn stalk can be used as a significant bioenergy resource. Novel concepts included in this dissertation include: (1) using corn stalk as a gasification fuel, (2) using corn stalk to generate activated carbon, (3) using activated carbon from corn stalk to adsorb organic pollutants, (4) using the gasification of corn stalk in a new process to dry moist biomass, (5) using the "partial" gasification of moist corn stalk in another new process to dry moist biomass in a single step. Each concept could be integrated with existing gasification technology to increase the efficient utilization of energy from biomass.

  20. Amino acid composition and biological effects of supplementing broad bean and corn proteins with Nigella sativa (black cumin) cake protein.

    Science.gov (United States)

    al-Gaby, A M

    1998-10-01

    The biological effects of supplementing broad bean (Vicia faba) or corn (Zea maize) meal protein with black cumin (Nigella sativa) cake protein as well as their amino acid composition were investigated. The percentage of total protein content of Nigella cake was 22.7%. Lysine is existent in abundant amounts in faba meal protein, while leucine is the most abundant in corn meal protein (chemical score = 156) and valine is higher in Nagella cake protein. compared with rats fed sole corn or faba meal protein, substitution of 25% of corn or faba meal protein with Nigella cake protein in the diet remarkably raised the growth rate of rats and resulted in significant higher levels of rat total serum lipids and triglycerides. Also, the supplemented diet caused significant increases in serum total protein and its two fractions albumin and globulin and insignificantly increase the activity of serum phosphatases and transaminases within normal ranges. The supplementation did not have any adverse nutritional effects in the levels of lipid fractions in the serum.

  1. Replacement of corn with babassu mesocarp flour in balanced rations for broilers in the period from 1 to 21 days

    Directory of Open Access Journals (Sweden)

    Flávia Luzia Rodrigues Fonseca

    2015-06-01

    Full Text Available The objective of this study was to evaluate the technical and economic feasibility of partial replacement of corn with babassu mesocarp flour in diets for broilers from one to 21 days of age. Two hundred male day-old chicks, from the Cobb 500® line, were distributed in a completely randomized design with four treatments (0%, 10%, 20% and 30% replacement of maize flour with the babassu mesocarp and five replicates were used comprising ten birds. Were evaluated performance (weight gain, feed intake, feed conversion and water intake, organ biometrics, metabolizability coefficients of dry matter (MCDM, crude protein (MCCP and gross energy (MCGE and cost of feeding diets containing babassu mesocarp flour (BMF. It was observed that the substitution of corn with BMF negatively influenced (P 0.05 the relative weights of the carcass, bursa, spleen, heart, gizzard or liver, nor the relative weight or length of the small intestine. The lower cost of feed per kg of weight gain was obtained with the diet containing 10% corn replacement with BMF, which resulted in a higher gross margin in relation to food. The replacement of corn with BMF in diets for broilers from one to 21 days of age proved to be technically feasible in 10%.

  2. Critical Period of Weed Control in Corn (Zea mays L.

    Directory of Open Access Journals (Sweden)

    R Alizadeh

    2012-06-01

    Full Text Available In order to determine the critical peroid of weed control (CPWC in grain maize, a field study was conducted in 2008 in Agricultural Research Station of Esfahan province using a randomized complete block design with 12 treatments and three replications. The treatments consisted of two different periods of weed interference, a critical weed-free period and a critical time of weed removal were imposed at V3, V6, V9, V12, and R1 (based on phenological stages of corn development with a weedy check and a weed-free check. The CPWC was determined by fitting non-linear regression models of logistic and gompertz equations to the data in range of 2.5, 5, 10 and 15% of acceptable yield loss levels. The CPWC was from 1- to 13-leaf stage (2-54 DAE, 3- to 11-leaf stage (10-46 DAE, 5- to 8-leaf stage (20-35 DAE and 6-leaf stage (27 DAE in Esfahan condition to prevent yield losses of 2.5%, 5% , 10% and 15% respectively.

  3. Comparison of Yield and Content of Anthocyanin of Purple Corn of Different Combining Types%紫玉米不同组配方式的花青素含量及产量比较

    Institute of Scientific and Technical Information of China (English)

    史振声; 贾森

    2012-01-01

    以18个玉米自交系组配成的16个紫玉米杂交组合为试验材料,研究不同组配方式配制的紫玉米籽粒、穗轴、苞叶、雄穗花青素含量及花青素产量.结果表明:(1)不同组配方式的紫玉米籽粒花青素含量、籽粒产量及籽粒花青素产量差异均较大.深紫玉米×深紫玉米,花青素含量最高;深紫非糯玉米×糯玉米,籽粒产量最高;深紫玉米×深紫玉米,籽粒花青素产量最高.(2)深紫玉米×深紫玉米,穗轴花青素含量、花青素产量最高;非紫苞叶×深紫苞叶,紫玉米苞叶花青素含量、花青素产量最高;非紫雄穗×深紫雄穗,紫玉米雄穗花青素含量、花青素产量最高.(3)穗轴花青素含量与籽粒花青素含量呈极显著正相关;雄穗花青素含量与苞叶花青素含量呈极显著正相关.(4)紫玉米花青素总产量(籽粒、稳轴、苞叶、雄穗)为:深紫×深紫>深紫×浅紫>非紫×深紫>浅紫×非紫;籽粒花青素产量对总花青素产量贡献最大.%Content and yield of anthocyanin in grain, cob, bract and tassel of purple corn of different combining types were studied using 16 purple corn hybrids grouped by 18 maize inbred lines. The results showed that;l. Differences of content of grain anthocyanin, grain yield and grain anthocyanin yield of purple corn of different combining types were all great. Content of anthocyanin of Deep Purple corn × Deep Purple corn was the highest; grain yield of Deep Purple non-waxy corn × waxy corn was the highest; yield of grain anthocyanin of Deep Purple corn × Deep Purple corn was the highest. 2. Yield and content of cob anthocyanin of Deep Purple corn x Deep Purple corn were both the highest; content and yield of bract anthocyanin of Purple non-bract corn x Deep Purple bract corn were both the highest; content and yield of tassel anthocyanin of Purple non-tassel corn × Deep Purple tassel corn were both the highest. 3. Content of cob and grain

  4. The role of maize root size in phosphorus uptake and productivity of maize/faba bean and maize/wheat intercropping systems.

    Science.gov (United States)

    Zhang, Yikai; Chen, Fanjun; Li, Long; Chen, Yanhua; Liu, Bingran; Zhou, Yuling; Yuan, Lixing; Zhang, Fusuo; Mi, Guohua

    2012-11-01

    Interspecific root/rhizosphere interactions affect phosphorus (P) uptake and the productivity of maize/faba bean and maize/wheat intercropping systems. The aim of these experiments was to determine whether manipulation of maize root growth could improve the productivity of the two intercropping systems. Two near isogenic maize hybrids (the larger-rooted T149 and smaller-rooted T222) were intercropped with faba bean and wheat, under conditions of high- and low-P availability. The larger-rooted T149 showed greater competitive ability than the smaller-rooted T222 in both maize/faba bean and maize/wheat intercropping systems. The higher competitive ability of T149 improved the productivity of the maize/faba bean intercropping system in P-sufficient conditions. In maize/wheat intercropping systems, root growth, shoot biomass, and P uptake of maize were inhibited by wheat, regardless of the P-supply. Compared with T222, the larger-rooted T149 suffered less in the intercropping systems. The total biomass of the maize/wheat intercropping system was higher for wheat/T149 than for wheat/T222 under low-P conditions. These data suggested that genetic improvement of maize root size could enhance maize growth and its ability to compete for P resources in maize/faba bean and maize/wheat intercropping systems. In addition, depending on the P availability, larger maize roots could increase the productivity of intercropping systems.

  5. The iojap gene in maize

    Energy Technology Data Exchange (ETDEWEB)

    Martienssen, Robert

    2001-12-01

    The classical maize mutant iojap (Iodent japonica) has variegated green and white leaves. Green sectors have cells with normal chloroplasts whereas white sectors have cells where plastids fail to differentiate. These mutant plastids, when transmitted through the female gametophyte, do not recover in the presence of wild type Iojap. We cloned the Ij locus, and we have investigated the mechanism of epigenetic inheritance and phenotypic expression. More recently, a modifier of this type of variegation, ''Inhibitor of striate'', has also been cloned. Both the iojap and inhibitor of striate proteins have homologs in bacteria and are members of ancient conserved families found in multiple species. These tools can be used to address fundamental questions of inheritance and variegation associated with this classical conundrum of maize genetics. Since the work of Rhoades there has been considerable speculation concerning the nature of the Iojap gene product, the origin of leaf variegation and the mechanism behind the material inheritance of defective plastids. This has made Iojap a textbook paradigm for cytoplasmic inheritance and nuclear-organellar interaction for almost 50 years. Cloning of the Iojap gene in maize, and homologs in other plants and bacteria, provides a new means to address the origin of heteroplastidity, variegation and cytoplasmic inheritance in higher plants.

  6. Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Luis H. León Mendoza

    2014-12-01

    Full Text Available The yellow maize is the third most important crop in Peru and part of the chain maize-poultry-pig, significant impact on the national economic and social activity, however, in 2011, only 40% of the corn offered corresponded to the domestic industry. Looking for alternatives to reduce the use of chemical fertilizers, have performed investigations with plant growth promoting rhizobacteria. Bacteria were isolated from the rhizosphere of maize from districts Monsefú and Reque, Lambayeque. Dilution was made into sterile saline 0.87% NaCl w/v and plated on MacConkey agar, incubating at 30°C for 48 hours. 269 pure cultures of bacteria were obtained, the biochemical reaction was investigated in agar Triple sugar iron agar Iron Lysine, agar Citrate Simons, peptone broth, red broth methyl Voges-Proskauer and nitrate broth, was identified 66% as Enterobacteriaceae of genera Pantoea (49%, Klebsiella (17%, Kluyvera (16%, Serratia (11%, Citrobacter (4% and Hafnia (3%. The native enterobacteria were quantified to 31.67 ppm of fixed nitrogen as ammonia; 54.25 ppm indole acetic acid and 4,78 ppm solubilized phosphorus, activity proteolytic and chitinolytic and antagonistic activity of Fusarium verticillioides were also determined. 16% of native enterobacteria did not affect the emergence of hard yellow maize, 77% affected positively and 7% affected negatively. In turn, none bacteria affected survival. Was demonstrated the potential plant growth promoter of enterobacteria isolated from field crops in the region of Lambayeque.

  7. 玉米及其制品质量安全风险及控制%Safety Risk and Its Control of Corn and Corn Products

    Institute of Scientific and Technical Information of China (English)

    赵久然; 刘月娥

    2016-01-01

    玉米是全世界也是我国种植范围最广、总产量最大、用途最多的粮食作物。玉米按收获物和用途可分为籽粒用玉米、鲜食玉米、青贮玉米等3类,其中籽粒用玉米是我国玉米的主要产品。指出我国籽粒用玉米及其制品在质量安全方面存在的主要问题是真菌毒素污染,同时也在一定程度上存在农药残留和重金属污染等问题,并从玉米育种和作物栽培等方面给出相应的控制对策:1)选育并推广抗病虫害的玉米新品种,积极采取措施预防和防治病虫害。2)选育并推广脱水快、成熟度好、利于籽粒直收的玉米品种,规范玉米籽粒晾晒及储存过程。3)完善相关标准体系和检测体系。4)综合防治病虫草害,合理规范使用杀虫剂、杀菌剂、除草剂,生物防治为主,化学防治为辅,使用低毒农药。5)加强标准农田建设,减少面源污染,降低重金属在玉米中吸收和积累。%Corn is the world’s and China’s largest planted and most widely used crop,with the highest yield production among all the crops. According to the harvest parts and uses, corn can be divided into three types, which are grain corn, fresh corn and silage corn. Grain corn is one of the main products in China. This study points out that the main quality and safety problems of grain corn and its products in terms are mycotoxins contamination. Problems of pesticide residue and heavy metal pollution also exist to a certain extent . This study also give the corresponding control measures from the aspects of corn breeding and culti-vation, including:1) Breeding new maize cultivars of wide adaptability and resistance, and taking active measures to prevent and control diseases and insect pests. 2) Breeding and recommending good maturity and quick dehydration cultivars suitable for grain harvesting, and drying timely and storing standardly. 3) Improving relevant test and standard systems. 4

  8. WEATHER CONDITIONS OF MAIZE VEGETATION IN CONNECTION WITH THE PLANTING DATES IN THE ARID ZONE OF CENTRAL CISCAUCASUSIA

    Directory of Open Access Journals (Sweden)

    Kravchenko R. V.

    2016-02-01

    Full Text Available There were reviewed the results of the study of the dependence of main phenological phases of development of corn hybrids of different maturity groups of the selection of Krasnodar Research Institute of Agriculture named after P.P. Lukyanenko in the arid zone of Central Ciscaucasia (Ross 199, Ross 299, Krasnodar 382 and Krasnodar 410 and the AllRussian Research Institute of Corn (Mashuk 170, Newton, RIC 345 and Eric, as well as early-middle Rossiyskaya 1 from the changes of average daily air temperature of planting dates and preplant seed treatment by the preparation "TMTD-plus", containing the growth promoter called Krezatsin in its composition. The studies were conducted in accordance with the thematic plan of scientific researches of the department of crop and forage production of Stavropol State Agrarian University. The technology of maize growing on the experimental plot corresponds to the standard technology for this area and culture. The sowing was performed in three stages. The first (early sowing period was at t = + 7 ... +8 ° C. The second (recommended – was at t = + 10 ... + 12 ° C. The third (later sowing period was at t = +15 ° C. There was identified a high inverse correlation between the average daily air temperature and the duration of the intraphase periods of maize propagation. Thus, at the shifting of maize sowing dates with the second half of May on the second half of April we have optimization of heat regime in the generative period and rising of the efficiency of use of thermal resources of the region. There was marked the acceleration of development of corn seedlings when the average daily air temperatures was up to 12 ° C at the samples with the application of the disinfectant TMTD-plus

  9. HYDROTHERMAL CONDITIONS OF MAIZE VEGETATION DUE TO THE DATES OF PLANTING IN THE SUFFICIENT MOISTURE ZONE OF CENTRAL CISCAUCASIA

    Directory of Open Access Journals (Sweden)

    Kravchenko R. V.

    2016-02-01

    Full Text Available There were reviewed the results of the study of the dependence of main of phenological phases of development of corn hybrids of different maturity groups of selection of Krasnodar Research Institute of Agriculture named. P.P. Lukyanenko in a zone of sufficient moisture of Central Ciscaucasia (Ross 199, Ross 299, Krasnodar 382 and Krasnodar 410 and the All-Russian Research Institute of Corn (Mashuk 170, Newton, RIC 345 and Eric, as well as early-middle middle Rossiyskaya 1 from the change of average daily air temperature at different dates of sowing and preplant seed treatment by the preparation "TMTD-plus" containing the growth promoter called Krezatsin in its composition. The studies were conducted in accordance with the thematic plan of scientific researches of the department of crop and forage production of Stavropol State Agrarian University. The technology of maize growing on the experimental plot corresponds to the standard technology for this area and culture. The sowing was performed in three stages. The first (early sowing period was at t = + 7 ... +8 ° C. The second (recommended – was at t = + 10 ... + 12 ° C. The third (later sowing period was carried out at t = +15 ° C. There was identified a high inverse correlation between the average daily air temperature and the duration of the intraphase periods of maize propagation. Thus, at the shifting of maize sowing dates with the second half of may on the second half of April, the optimization of heat regime in the generative period and rising of efficiency of use of thermal of resources of the region. There was marked the acceleration of development of corn seedlings when the average daily air temperatures was up to +12 ° C at the samples with the application of the disinfectant TMTD-plus

  10. The key role of cultural preservation in maize diversity conservation in the argentine yungas.

    Science.gov (United States)

    Hilgert, Norma I; Zamudio, Fernando; Furlan, Violeta; Cariola, Lucía

    2013-01-01

    Farmers' decisions on what to grow and why can contribute in understanding the conservation of agrobiodiversity. Culture and ethnicity are indicated as first-class factors leading preservation of heirloom cultivars but this has been little considered in studies examining factors that influence the loss or preservation of agrobiodiversity. We propose that corn's ethnotaxa of less diverse uses, which are also key partners in local cultural reproduction, are usually cultivated by a few households. We analyse if there is a relationship between uses and richness of cultivated ethnotaxa at household level and describe corn's medicinal and ritual uses. We found 25 cultivated ethnotaxa, heterogeneously distributed in the region, and we also found that ethnotaxa with less diverse uses are cultivated in fewer households. We identified that, at regional scale, richness is related with food use diversity. The most frequently cited medicinal uses were urinary and tract infections, diarrhoea, and liver disorders. Medicinal recipes involve combinations with other elements. Maize is an indispensable resource in the rituals that propitiate productive activity, to augur prosperity or misfortune according to signals. We have identified the vulnerability in preserving the richness of corn in the region and the factors that shape its cultivation at different scales.

  11. The Key Role of Cultural Preservation in Maize Diversity Conservation in the Argentine Yungas

    Science.gov (United States)

    Hilgert, Norma I.; Zamudio, Fernando; Cariola, Lucía

    2013-01-01

    Farmers' decisions on what to grow and why can contribute in understanding the conservation of agrobiodiversity. Culture and ethnicity are indicated as first-class factors leading preservation of heirloom cultivars but this has been little considered in studies examining factors that influence the loss or preservation of agrobiodiversity. We propose that corn's ethnotaxa of less diverse uses, which are also key partners in local cultural reproduction, are usually cultivated by a few households. We analyse if there is a relationship between uses and richness of cultivated ethnotaxa at household level and describe corn's medicinal and ritual uses. We found 25 cultivated ethnotaxa, heterogeneously distributed in the region, and we also found that ethnotaxa with less diverse uses are cultivated in fewer households. We identified that, at regional scale, richness is related with food use diversity. The most frequently cited medicinal uses were urinary and tract infections, diarrhoea, and liver disorders. Medicinal recipes involve combinations with other elements. Maize is an indispensable resource in the rituals that propitiate productive activity, to augur prosperity or misfortune according to signals. We have identified the vulnerability in preserving the richness of corn in the region and the factors that shape its cultivation at different scales. PMID:24078829

  12. Possible sources of archaeological maize found in Chaco Canyon and Aztec Ruin, New Mexico

    Science.gov (United States)

    Benson, L.V.; Stein, J.R.; Taylor, H.E.

    2009-01-01

    Maize played a major role in Chaco's interaction with outlying communities in the southern Colorado Plateau. This paper seeks to determine where archaeological corn cobs brought to Chaco Canyon were grown. Strontium-isotope and trace-metal ratios of 180 soil-water and 18 surface-water sites in the Southern Colorado Plateau have revealed possible source areas for some of 37 archaeological corn cobs from Chaco Canyon and 10 archaeological corn cobs from Aztec Ruin, New Mexico. The most probable source areas for cobs that predate the middle-12th-century drought include several Upper Rio Chaco sites (not including Chaco Canyon). There are many potential source areas for cobs that date to the late A.D. 1100s and early 1200s, all of which lie in the eastern part of the study area. Some Athapascan-age cobs have potential source areas in the Totah, Lobo Mesa, and Dinetah regions. One Gallo Cliff Dwelling cob has a strontium-isotope ratio that exceeds all measured soil-water values. Field sites for this cob may exist in association with Paleozoic and Precambrian rocks found 80-90 km from Chaco Canyon. Potential source areas for most Aztec Ruin cobs (many of which were found in rooms dating to the first half of the 13th-century) appear to be associated with a loess deposit that blankets the Mesa Verde and McElmo Dome regions.

  13. Evaluation of the compositional and nutritional values of phytase transgenic corn to conventional corn in roosters.

    Science.gov (United States)

    Gao, C Q; Ma, Q G; Ji, C; Luo, X G; Tang, H F; Wei, Y M

    2012-05-01

    Three experiments were conducted to evaluate the compositional and nutritional values of corn grains [phytase transgenic corn (PTC) and isogenic conventional corn (CC)] and compare the efficacy of corn-based phytase and extraneous microbial phytase for enhancing the utilization of phytate phosphorus (P) in single corn or corn-soybean mixed meals (corn:soybean = 2.5:1, wt:wt) fed to roosters. Following a 48-h fasting period, 16 roosters were given 50 g of each sample via crop intubation and excreta were collected for 48 h. Nitrogen-free and phosphorus-free diets were used to evaluate endogenous amino acid and endogenous P losses, respectively. Chemical composition was not different between PTC and CC, whereas the phytase content for PTC was greater than CC (8,047 vs. 37 FTU/kg of corn, DM basis; P 0.05) between roosters fed PTC and extraneous microbial phytase in equivalent FTU/kg of diets. The results of this study indicated that the chemical composition, TME, and true amino acid availability in PTC are essentially equivalent to that in CC, and the true P utilization for roosters is higher in PTC than in CC. Corn expressing phytase is as efficacious as equivalent microbial phytase when supplemented in corn-soybean diets for chickens.

  14. Production of thermotolerant entomopathogenic Isaria fumosorosea SFP-198 conidia in corn-corn oil mixture.

    Science.gov (United States)

    Kim, Jae Su; Je, Yeon Ho; Roh, Jong Yul

    2010-04-01

    Low thermotolerance of entomopathogenic fungi is a major impediment to long-term storage and effective application of these biopesticides under seasonal high temperatures. The effects of high temperatures on the viability of an entomopathogenic fungus, Isaria fumosorosea SFP-198 (KCTC 0499BP), produced on different substrates amended with various additives were explored. Ground corn was found to be superior in producing the most thermotolerant conidia compared to yellow soybean, red kidney bean, and rice in a polyethylene bag production system. Using ground corn mixed with corn oil as a substrate resulted in only 7% reduction in germination compared to ground corn alone (67% reduction) after exposure of conidia to 50 degrees C for 2 h. Corn oil as an additive for ground corn was followed by inorganic salts (KCl and NaCl), carbohydrates (sucrose and dextrin), a sugar alcohol (sorbitol), and plant oils (soybean oil and cotton seed oil) in ability to improve conidial thermotolerance. Unsaturated fatty acids, such as linoleic acid and oleic acid, the main components of corn oil, served as effective additives for conidial thermotolerance in a dosage-dependent manner, possibly explaining the improvement by corn oil. This finding suggests that the corn-corn oil mixture can be used to produce highly thermotolerant SFP-198 conidia and provides the relation of unsaturated fatty acids as substrates with conidial thermotolerance.

  15. Use of tropical maize for bioethanol production

    Science.gov (United States)

    Tropical maize is an alternative energy crop being considered as a feedstock for bioethanol production in the North Central and Midwest United States. Tropical maize is advantageous because it produces large amounts of soluble sugars in its stalks, creates a large amount of biomass, and requires lo...

  16. Towards the improvement of maize in Nigeria

    NARCIS (Netherlands)

    Eijnatten, van C.L.M.

    1965-01-01

    Approximately 600,000 tons of maize were produced annually in Nigeria. Maize was the main grain crop in southern Nigeria and its production in the middle belt was rapidly increasing. Most of the crop was eaten as dry grain, a little before maturity. A preference was recorded for floury varieties of

  17. ZP maize hybrids as silage raw material

    Directory of Open Access Journals (Sweden)

    Terzić Dušanka

    2012-01-01

    Full Text Available Due to its yields, biomass quality and biomass suitability for ensiling, diversity of use in feedstuff, maize is one of the most important forage plants. In comparison with other forage plants, the production of silage maize results in the greatest accumulation of solar energy per area unit. The positive correlation between a high density of net energy and the concentration of net energy as well as between digestibility of organic matter and the concentration of net energy is achieved in the produced biomass of maize plant. The yield of silage maize in dependence on the genetic potential of yield and agroecological conditions of the production ranges from 12 to 25 tonnes of the total dry matter per hectare at physiological maturity for ensiling with the dry matter content of 35-42%. This study presents results obtained in the long-term scientific and research programme on the improvement of ZP maize hybrids utilisation carried out in the Department of Technological Research at the Maize Research Institute Zemun Polje. The overall presentation of biomass quality parameters of six ZP maize hybrids (ZP 158, ZP 173/8, ZP 377, ZP 440, ZP 555 and ZP 679 of different genetic backgrounds intended for silage production is given in this study. Besides, interrelationships among these factors as well as their effects on maize biomass digestibility were established.

  18. Exploring maize-legume intercropping systems in Southwest Mexico

    NARCIS (Netherlands)

    Flores-Sanchez, D.; Pastor, A.V.; Lantinga, E.A.; Rossing, W.A.H.; Kropff, M.J.

    2013-01-01

    Maize yields in continuous maize production systems of smallholders in the Costa Chica, a region in Southwest Mexico, are low despite consistent inputs of fertilizers and herbicides. This study was aimed at investigating the prospects of intercropping maize (Zea mays L.) and maize-roselle (Hibiscus

  19. Multipass rotary shear comminution process to produce corn stover particles

    Energy Technology Data Exchange (ETDEWEB)

    Dooley, James H; Lanning, David N

    2015-04-14

    A process of comminution of corn stover having a grain direction to produce a mixture of corn stover, by feeding the corn stover in a direction of travel substantially randomly to the grain direction one or more times through a counter rotating pair of intermeshing arrays of cutting discs (D) arrayed axially perpendicular to the direction of corn stover travel.

  20. Pilot process for decolorizing/deodorizing commercial corn zein products

    Science.gov (United States)

    Corn zein is the major protein component of ground corn, and co-products of the corn ethanol industry which includes distiller’s dried grains and corn gluten meal. Zein products generated from those materials all possess some degree of yellow color and off-odor that deters their usage in food syste...

  1. Utilization of corn fiber for production of schizophyllan

    Science.gov (United States)

    Corn fiber is an abundant lignocellulosic biomass resource produced during the wet milling of corn. Although corn fiber is recalcitrant to enzymatic digestion, the fungus Schizophyllum commune was able to directly utilize corn fiber for production of the valuable bioproduct, schizophyllan. Schizophy...

  2. On-Farm Validation of Alfalfa N Credits to Corn

    Science.gov (United States)

    Rotating alfalfa with corn is useful for reducing soil erosion, enhancing soil tilth and carbon storage, reducing weed seedbanks, disrupting the life cycles of disease and insect pests of corn, and supplying nitrogen (N) to the subsequent corn crop. To adjust N fertilizer rates for corn following al...

  3. ADVANCES IN TRANSGENIC MAIZE FOR QUALITY IMPROVEMENT

    Directory of Open Access Journals (Sweden)

    M.Rajendar Reddy

    2015-12-01

    Full Text Available Maize (Zea mays is a major food and animal feed worldwide and occupies a relevant place in the world economy and trade as an industrial grain crop. Currently more than 70% of maize production is used for food and feed; therefore, knowledge of genes involved in grain structure and chemical is important for improving the nutritional and food-making properties of maize. It is a good source of carbohydrates, fats, proteins, vitamins and minerals but deficient in two essential amino acids, Viz., lysine and tryptophan. To overcome this problem and to improve the above quality characters the maize breeders have followed different strategies like opaque 2, QPM and development of transgenic maize with improved quality characters. Finally we can conclude that the conventional breeding techniques and now plant biotechnology are helping meet the growing demand for food production, nutrition security while preserving our environment for future generations

  4. Nontarget organism effects tests on eCry3.1Ab and their application to the ecological risk assessment for cultivation of Event 5307 maize.

    Science.gov (United States)

    Burns, Andrea; Raybould, Alan

    2014-12-01

    Event 5307 transgenic maize produces the novel insecticidal protein eCry3.1Ab, which is active against certain coleopteran pests such as Western corn rootworm (Diabrotica virgifera virgifera). Laboratory tests with representative nontarget organisms (NTOs) were conducted to test the hypothesis of no adverse ecological effects of cultivating Event 5307 maize. Estimates of environmental eCry3.1Ab concentrations for each NTO were calculated from the concentrations of eCry3.1Ab produced by 5307 maize in relevant plant tissues. Nontarget organisms were exposed to diets containing eCry3.1Ab or diets comprising Event 5307 maize tissue and evaluated for effects compared to control groups. No statistically significant differences in survival were observed between the control group and the group exposed to eCry3.1Ab in any organism tested. Measured eCry3.1Ab concentrations in the laboratory studies were equal to or greater than the most conservative estimates of environmental exposure. The laboratory studies corroborate the hypothesis of negligible ecological risk from the cultivation of 5307 maize.

  5. Nutrition Characters of Sweet Corns in Kernel Milky Maturity

    Institute of Scientific and Technical Information of China (English)

    Wu Moucheng; Chen Xiaoyi

    2000-01-01

    Three corn varieties, supper-sweet corn (S), standard-sweet corn (M), common corn(C) were used for nutritional composition measurement during kernel milky maturity.The variations of protein, vitamin, total sugar showed as parabola. Mineral elements and fiber increase and reducing sugar decreased gradually. Total sugar, protein and fat in the sweet corn were much richer than those in common corn. VE and Vc were very plentiful, and lysine was high. Proper harvest time of sweet corn M and S were DAP (days after pollination)19-21 ,and DAP 18-21 respectively.

  6. Feasible Management of Southern Corn Leaf Blight via Induction of Systemic Resistance by Bacillus cereus C1L in Combination with Reduced Use of Dithiocarbamate Fungicides.

    Science.gov (United States)

    Lai, Yi-Ru; Lin, Pei-Yu; Chen, Chao-Ying; Huang, Chien-Jui

    2016-10-01

    Dithiocarbamate fungicides such as maneb and mancozeb are widely used nonsystemic protectant fungicides to control various plant fungal diseases. Dithiocarbamate fungicides should be frequently applied to achieve optimal efficacy of disease control and avoid either decline in effectiveness or wash-off from leaf surface. Dithiocarbamates are of low resistance risk but have the potential to cause human neurological diseases. The objective of this study was to develop a strategy to effectively control plant disease with reduced use of dithiocarbamtes. Southern corn leaf blight was the model pathosystem for the investigation. When corn plants were drench-treated with Bacillus cereus C1L, a rhizobacterium able to induce systemic resistance in corn plants against southern leaf blight, frequency of spraying dithiocarbamate fungicides could be decreased. The treatment of B. cereus C1L was able to protect maize from southern leaf blight while residues of dithiocarbamates on leaf surface were too low to provide sufficient protection. On the other hand, frequent sprays of mancozeb slightly but significantly reduced growth of corn plants under natural conditions. In contrast, application of B. cereus C1L can significantly promote growth of corn plants whether sprayed with mancozeb or not. Our results provide the information that plant disease can be well controlled by rhizobacteria-mediated induced systemic resistance in combination with reduced but appropriate application of dithiocarbamate fungicides just before a heavy infection period. An appropriate use of rhizobacteria can enhance plant growth and help plants overcome negative effects caused by dithiocarbamates.

  7. Feasible Management of Southern Corn Leaf Blight via Induction of Systemic Resistance by Bacillus cereus C1L in Combination with Reduced Use of Dithiocarbamate Fungicides

    Directory of Open Access Journals (Sweden)

    Yi-Ru Lai

    2016-10-01

    Full Text Available Dithiocarbamate fungicides such as maneb and mancozeb are widely used nonsystemic protectant fungicides to control various plant fungal diseases. Dithiocarbamate fungicides should be frequently applied to achieve optimal efficacy of disease control and avoid either decline in effectiveness or wash-off from leaf surface. Dithiocarbamates are of low resistance risk but have the potential to cause human neurological diseases. The objective of this study was to develop a strategy to effectively control plant disease with reduced use of dithiocarbamtes. Southern corn leaf blight was the model pathosystem for the investigation. When corn plants were drench-treated with Bacillus cereus C1L, a rhizobacterium able to induce systemic resistance in corn plants against southern leaf blight, frequency of spraying dithiocarbamate fungicides could be decreased. The treatment of B. cereus C1L was able to protect maize from southern leaf blight while residues of dithiocarbamates on leaf surface were too low to provide sufficient protection. On the other hand, frequent sprays of mancozeb slightly but significantly reduced growth of corn plants under natural conditions. In contrast, application of B. cereus C1L can significantly promote growth of corn plants whether sprayed with mancozeb or not. Our results provide the information that plant disease can be well controlled by rhizobacteria-mediated induced systemic resistance in combination with reduced but appropriate application of dithiocarbamate fungicides just before a heavy infection period. An appropriate use of rhizobacteria can enhance plant growth and help plants overcome negative effects caused by dithiocarbamates.

  8. Feasible Management of Southern Corn Leaf Blight via Induction of Systemic Resistance by Bacillus cereus C1L in Combination with Reduced Use of Dithiocarbamate Fungicides

    Science.gov (United States)

    Lai, Yi-Ru; Lin, Pei-Yu; Chen, Chao-Ying; Huang, Chien-Jui

    2016-01-01

    Dithiocarbamate fungicides such as maneb and mancozeb are widely used nonsystemic protectant fungicides to control various plant fungal diseases. Dithiocarbamate fungicides should be frequently applied to achieve optimal efficacy of disease control and avoid either decline in effectiveness or wash-off from leaf surface. Dithiocarbamates are of low resistance risk but have the potential to cause human neurological diseases. The objective of this study was to develop a strategy to effectively control plant disease with reduced use of dithiocarbamtes. Southern corn leaf blight was the model pathosystem for the investigation. When corn plants were drench-treated with Bacillus cereus C1L, a rhizobacterium able to induce systemic resistance in corn plants against southern leaf blight, frequency of spraying dithiocarbamate fungicides could be decreased. The treatment of B. cereus C1L was able to protect maize from southern leaf blight while residues of dithiocarbamates on leaf surface were too low to provide sufficient protection. On the other hand, frequent sprays of mancozeb slightly but significantly reduced growth of corn plants under natural conditions. In contrast, application of B. cereus C1L can significantly promote growth of corn plants whether sprayed with mancozeb or not. Our results provide the information that plant disease can be well controlled by rhizobacteria-mediated induced systemic resistance in combination with reduced but appropriate application of dithiocarbamate fungicides just before a heavy infection period. An appropriate use of rhizobacteria can enhance plant growth and help plants overcome negative effects caused by dithiocarbamates. PMID:27721698

  9. Distribution of disease symptoms and mycotoxins in maize ears infected by Fusarium culmorum and Fusarium graminearum.

    Science.gov (United States)

    Oldenburg, Elisabeth; Ellner, Frank

    2015-08-01

    Red ear rot an important disease of maize cultivated in Europe is caused by toxigenic Fusarium species like Fusarium graminearum and Fusarium culmorum. To get detailed information on the time course of the infection process leading to the accumulation of Fusarium mycotoxins in maize ears, a field study was conducted over 2 years with two maize varieties, which were inoculated with F. culmorum or F. graminearum isolates at the stage of female flowering. Every fortnight after inoculation, infection and contamination progress in the ears was followed by visually evaluating disease signs and analysing Fusarium toxin concentrations in the infected ear tissues. In principle, infection and mycotoxin distribution were similar in respect of pathogens, varieties, and years. External infection symptoms showing some small pale or brown-marbled kernels with dark brown pedicels were mainly seen at the ear tip, whereas internal infection symptoms on the rachis were much more pronounced and spread in the upper half showing greyish brownish or pink discoloration of the pith. Well correlated with disease symptoms, a top-down gradient from high to low toxin levels within the ear with considerably higher concentrations in the rachis compared with the kernels was observed. It is suggested that both Fusarium pathogens primarily infect the rachis from the tip toward the bottom, whereas the kernels are subsequently infected via the rachillae connected to the rachis. A special focus on the pronounced disease symptoms visible in the rachis may be an approach to improve the evaluation of maize-genotype susceptibility against red ear rot pathogens. It has to be underlined that the accumulation of Fusarium mycotoxins in the rachis greatly accelerated 6 weeks after inoculation; therefore, highest contamination risk is indicated for feedstuffs containing large amounts of rachis (e.g., corn cob mix), especially when cut late in growing season.

  10. Remarkable variation in maize genome structure inferred from haplotype diversity at the bz locus.

    Science.gov (United States)

    Wang, Qinghua; Dooner, Hugo K

    2006-11-21

    Maize is probably the most diverse of all crop species. Unexpectedly large differences among haplotypes were first revealed in a comparison of the bz genomic regions of two different inbred lines, McC and B73. Retrotransposon clusters, which comprise most of the repetitive DNA in maize, varied markedly in makeup, and location relative to the genes in the region and genic sequences, later shown to be carried by two helitron transposons, also differed between the inbreds. Thus, the allelic bz regions of these Corn Belt inbreds shared only a minority of the total sequence. To investigate further the variation caused by retrotransposons, helitrons, and other insertions, we have analyzed the organization of the bz genomic region in five additional cultivars selected because of their geographic and genetic diversity: the inbreds A188, CML258, and I137TN, and the land races Coroico and NalTel. This vertical comparison has revealed the existence of several new helitrons, new retrotransposons, members of every superfamily of DNA transposons, numerous miniature elements, and novel insertions flanked at either end by TA repeats, which we call TAFTs (TA-flanked transposons). The extent of variation in the region is remarkable. In pairwise comparisons of eight bz haplotypes, the percentage of shared sequences ranges from 25% to 84%. Chimeric haplotypes were identified that combine retrotransposon clusters found in different haplotypes. We propose that recombination in the common gene space greatly amplifies the variability produced by the retrotransposition explosion in the maize ancestry, creating the heterogeneity in genome organization found in modern maize.

  11. Cassava and corn starch in maltodextrin production

    Directory of Open Access Journals (Sweden)

    Geovana Rocha Plácido Moore

    2005-08-01

    Full Text Available Maltodextrin was produced from cassava and corn starch by enzymatic hydrolysis with alpha-amylase. The cassava starch hydrolysis rate was higher than that of corn starches in maltodextrin production with shorter dextrose equivalent (DE. DE values do not show directly the nature of the obtained oligosaccharides. Maltodextrin produced from cassava and corn starch was analysed by high performance liquid chromatography (HPLC, and the analysis showed that maltodextrin production differs according to the source of the starch. This is important in defining the application of the maltodextrin, according to its desired function.

  12. ALKALINE PULP OF CORN STALKS

    Institute of Scientific and Technical Information of China (English)

    M.SarwarJalaan; M.AN.Russell; S.A.N.Shamim; A.I.Mostafa; Md.AbdulQuaiyyum

    2004-01-01

    Pulping of corn stalks was studied in soda,soda-anthraquinone (AQ), kraft and kraft-AQprocesses. The time, temperature and alkaliconcentration were varied in soda process. In respectto kappa number and pulp yield, 1 hour cooking at1400C in 14% alkali were best conditions for cornstalks pulping. Pulp yield was increased by 5.5% andkappa number was reduced by 4.4 points with anaddition of 0.05% AQ in the soda liquor. Breakinglength was better in soda-AQ process than sodaprocess but tear strength was inferior. In the kraftprocess, pulp yield was increased with increasingsulphidity and decreasing active alkali. Theeffectiveness of AQ in the low and high sulphiditykraft process was studied. Results showed that AQwas more effective in low sulphidity than highsulphidity. Strength properties in kraft processeswere better than the soda and soda-AQ processes.

  13. Effects of Interplanting Flowering Plants on the Biological Control of Corn Earworm (Lepidoptera: Noctuidae) and Thrips (Thysanoptera: Thripidae) in Sweet Corn.

    Science.gov (United States)

    Manandhar, Roshan; Wright, Mark G

    2016-02-01

    Natural enemy exploitation of food resources and alternative hosts in noncrop vegetation has been shown to be an effective means of enhancing natural enemy populations in diversified agro-ecosystem. Field trials were conducted in Hawaii to examine effects of interplanting flowering plants on 1) parasitism of corn earworm, Helicoverpa zea (Boddie) eggs by Trichogramma spp., and 2) abundance of Orius spp. in relation to prey (H. zea eggs and thrips [primarily, Frankliniella occidentalis (Pergande) and Frankliniella williamsi Hood]). Sweet corn (maize), Zea mays L., was interplanted with three flowering plants, buckwheat, Fagopyrum esculentum Moench, cowpea, Vigna unguiculata (L.), and sunn hemp, Crotolaria juncea L., at 2:1 and 4:1 (corn: flowering plant) ratios in 2009 and 2010, respectively. In 2009, the abundance of Orius spp. was significantly greater in the buckwheat-interplanted treatment compared to the monocrop control at similar levels of prey availability, indicating buckwheat flowers might have provided both prey and nectar resources. In 2010, cowpea and sunn hemp flowering plants provided a source of an alternate host insect's eggs for Trichogramma spp. oviposition, resulting in significantly higher parasitism of H. zea eggs in the cowpea- and sunn hemp-interplanted treatments compared to the monocrop control. Despite of differences in pest and natural enemy interactions in two field trials, our findings suggested that provisioning of an alternate host insect's eggs through flowering plants is an effective means for enhancing Trichogramma spp. and provisioning of both nectar and prey resources through flowering plants is important for enhancing predation by Orius spp.

  14. Spring harvest of corn stover

    Energy Technology Data Exchange (ETDEWEB)

    Lizotte, P.L. [Laval Univ., Quebec City, PQ (Canada). Dept. des sols et de genie agroalimentaire; Savoie, P. [Agriculture and Agri-Food Canada, Quebec City, PQ (Canada)

    2010-07-01

    Corn stover is typically left behind in the field after grain harvest. Although part of the stover should remain in the field for soil organic matter renewal and erosion protection, half of the stover could be removed sustainably. This represents about one million t dry matter (DM) of stover per year in the province of Quebec. Stover harvested in the fall is very wet. While there are applications for wet stover, the available markets currently require a dry product. Preliminary measurements have shown that stover left in the field throughout the winter becomes very dry, and a considerable amount would still be harvestable in the spring. In the spring of 2009, corn stover was harvested at 2 sites, each subdivided into 2 parcels. The first parcel was cut and raked in the fall of 2008 (fall parcel), while the second parcel was cut and raked in spring 2009. Fibre from both parcels was baled in the spring 2009. At the first site, a large square baler was used in late April to produce bales measuring 0.8 m x 0.9 m x 1.8 m. On the second site a round baler was used in late May to produce bales of 1.2 m in width by 1.45 m in diameter. On the second site, a small square baler was also used to produce bales of 0.35 m x 0.45 m x 0.60 m (spring cutting only). With the large square baler, an average of 3.9 t DM/ha was harvested equally on the fall parcel and the spring parcel, representing a 48 per cent recovery of biomass based on stover yields.

  15. INTEGRATED WEED CONTROL IN MAIZE.

    Science.gov (United States)

    Latré, J; Dewitte, K; Derycke, V; De Roo, B; Haesaert, G

    2015-01-01

    Integrated pest management has been implemented as a general practice by EU legislation. As weed control actually is the most important crop protection measure in maize for Western Europe, the new legislation will have its impact. The question is of course which systems can be successfully implemented in practice with respect to labour efficiency and economical parameters. During 3 successive growing seasons (2007, 2008, 2009) weed control in maize was evaluated, the main focus was put on different techniques of integrated weed control and was compared with chemical weed control. Additionally, during 4 successive growing seasons (2011, 2012, 2013 and 2014) two objects based on integrated weed control and two objects based on mechanical weed control were compared to about twenty different objects of conventional chemical weed control. One of the objects based on mechanical weed control consisted of treatment with the flex-tine harrow before and after emergence in combination with chemical weed control at a reduced rate in 3-4 leave stage. The second one consisted of broadcast mechanical treatments before and after emergence followed by a final in-row application of herbicides and an inter-row cultivation at 6-7(8) leave stage. All trials were conducted on the Experimental farm of Bottelare HoGent-UGent on a sandy loam soil. Maize was growing in 1/3 crop rotation. The effect on weed growth as well as the economic impact of the different applications was evaluated. Combining chemical and mechanical weed control is a possible option in conventional farming but the disadvantages must be taken into account. A better planned weed control based on the real present weed-population in combination with a carefully thought-out choice of herbicides should also be considered as an IPM--approach.

  16. Aquatic degradation of Cry1Ab protein and decomposition dynamics of transgenic corn leaves under controlled conditions.

    Science.gov (United States)

    Böttger, Rita; Schaller, Jörg; Lintow, Sven; Gert Dudel, E

    2015-03-01

    The increasing cultivation of genetically modified corn plants (Zea mays) during the last decades is suggested as a potential risk to the environment. One of these genetically modified variety expressed the insecticidal Cry1Ab protein originating from Bacillus thuringiensis (Bt), resulting in resistance against Ostrinia nubilalis, the European corn borer. Transgenic litter material is extensively studied regarding the decomposition in soils. However, only a few field studies analyzed the fate of the Cry1Ab protein and the impact of green and senescent leaf litter from corn on the decomposition rate and related ecosystem functions in aquatic environments. Consequently, a microbial litter decomposition experiment was conducted under controlled semi-natural conditions in batch culture using two maize varieties: one variety with Cry1Ab and another one with the appertaining Iso-line as control treatment. The results showed no significant differences between the treatment with Cry1Ab and the Iso-line regarding loss of total mass in dry weight of 43% for Iso-line and 45% for Bt-corn litter, lignin content increased to 137.5% (Iso-line) and 115.7% (Bt-corn), and phenol loss decreased by 53.6% (Iso-line), 62.2% (Bt-corn) during three weeks of the experiment. At the end of the experiment Cry1Ab protein was still detected with 6% of the initial concentration. A slightly but significant lower cellulose content was found for the Cry1Ab treatment compared to the Iso-line litter at the end of the experiment. The significant higher total protein (25%) and nitrogen (25%) content in Bt corn, most likely due to the additionally expression of the transgenic protein, may increase the microbial cellulose degradation and decrease microbial lignin degradation. In conclusion a relevant year by year input of protein and therefore nitrogen rich Bt corn litter into aquatic environments may affect the balanced nutrient turnover in aquatic ecosystems. Copyright © 2014 Elsevier Inc. All rights

  17. Thermal and rheological properties of nixtamalized maize starch.

    Science.gov (United States)

    Mendez-Montealvo, G; Sánchez-Rivera, M M; Paredes-López, O; Bello-Pérez, L A

    2006-12-15

    The effect of nixtamalization process on thermal and rheological characteristics of corn starch was studied. Starch of raw sample had higher gelatinization temperature than its raw counterpart, because, the Ca(2+) ions stabilize starch structure of nixtamalized sample; however, the enthalpy values were not different in both samples. The temperature of the phase transition of the retrograded starches (raw and nixtamalized) were not different at the storage times assessed, but the enthalpy values of the above mentioned transition was different, indicating a lower reorganization of the starch structure in the nixtamalized sample. The viscoamylographic profile showed differences between both starches, since raw starch had higher peak viscosity than the nixtamalized sample due to partial gelatinization of some granules during this heat treatment. Rheological test showed that at low temperature (25 degrees C) the raw and nixtamalized starches presented different behaviour; however, the elastic characteristic was more important in the starch gel structure. The nixtamalization process produced changes in thermal and rheological characteristics becoming important in those products elaborated from nixtamalized maize.

  18. A maize-specifically expressed gene cluster in Ustilago maydis.

    Science.gov (United States)

    Basse, Christoph W; Kolb, Sebastian; Kahmann, Regine

    2002-01-01

    The corn pathogen Ustilago maydis requires its host plant maize for development and completion of its sexual cycle. We have identified the fungal mig2-1 gene as being specifically expressed during this biotrophic stage. Intriguingly, mig2-1 is part of a gene cluster comprising five highly homologous and similarly regulated genes designated mig2-1 to mig2-5. Deletion analysis of the mig2-1 promoter provides evidence for negative and positive regulation. The predicted polypeptides of all five genes lack significant homologies to known genes but have characteristic N-terminal secretion sequences. The secretion signals of mig2-1 and mig2-5 were shown to be functional, and secretion of a full length Mig2-1-eGFP fusion protein to the extracellular space was demonstrated. The central domains of the Mig2 proteins are highly variable whereas the C-termini are strongly conserved and share a characteristic pattern of eight cysteine residues. The mig2 gene cluster was conserved in a wide collection of U. maydis strains. Interestingly, some U. maydis isolates from South America had lost the mig2-4 gene as a result of a homologous recombination event. Furthermore, the related Ustilago scitaminea strain, which is pathogenic on sugar cane, appears to lack the mig2 cluster. We describe a model of how the mig2 cluster might have evolved and discuss its possible role in governing host interaction.

  19. Effects of salts on the gelatinization and retrogradation properties of maize starch and waxy maize starch.

    Science.gov (United States)

    Wang, Wei; Zhou, Hongxian; Yang, Hong; Zhao, Siming; Liu, Youming; Liu, Ru

    2017-01-01

    The objective of this study was to evaluate the effects of salts on the gelatinization and retrogradation of maize and waxy maize starch. Experimental results showed that the salting-out or structure-making ions, such as F(-) and SO4(2-), decreased the swelling power, solubility and transparency of both starches, but increased the gelatinization temperature, enthalpy, and syneresis, due to the tendency of these ions to protect the hydrogen bond links among starch molecules. On the other hand, the salting-in or structure-breaking ions, such as I(-) and SCN(-), exhibited the opposite effects. Microscopic observations confirmed such effects of salts on both starches. Furthermore, the effects of salts were more significant on waxy maize and on normal maize starch. Generally, salts could significantly influence on the gelatinization and retrogradation of maize and waxy maize starch, following the order of the Hofmeister series.

  20. Specific energy requirement for compacting corn stover.

    Science.gov (United States)

    Mani, Sudhagar; Tabil, Lope G; Sokhansanj, Shahab

    2006-08-01

    Corn stover is a major crop residue for biomass conversion to produce chemicals and fuels. One of the problems associated with the supply of corn stover to conversion plants is the delivery of feedstock at a low cost. Corn stover has low bulk density and it is difficult to handle. In this study, chopped corn stover samples were compacted in a piston cylinder under three pressure levels (5, 10, 15 MPa) and at three moisture content levels (5%, 10%, 15% (wb)) to produce briquettes. The total energy requirement to compress and extrude briquette ranged from 12 to 30 MJ/t. The briquette density ranged from 650 to 950 kg/m3 increasing with pressure. Moisture content had also a significant effect on briquette density, durability and stability. Low moisture stover (5-10%) resulted in denser, more stable and more durable briquettes than high moisture stover (15%).

  1. Ethanol from corn silage. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Mehlberg, R.L.

    1981-10-01

    The corn silage to ethanol process is described. The process feed is corn silage preserved with sulfuric acid. No anaerobic ensilement is necessary since H/sub 2/SO/sub 4/ completely prevents microbial growth. The acidified corn silage is heated by steam injection as it is loaded into a batch reactor. The polysaccharides are hydrolyzed to xylose and glucose over a 6 to 8 hour period. Then the sugars are washed from the residual fibers over a 6 to 12 hour period with thin stillage or water. The hot, acidic syrup is then neutralized and cooled for fermentation. After fermentation the ethanol is distilled. The residual fibers containing the thin stillage, corn germ, cellulose, and lignin are unloaded from the reactor and dried with flue gases for animal feed.

  2. 21 CFR 184.1865 - Corn syrup.

    Science.gov (United States)

    2010-04-01

    ... “glucose syrup,” is obtained by partial hydrolysis of corn starch with safe and suitable acids or enzymes. It may also occur in the dehydrated form (dried glucose sirup). Depending on the degree of...

  3. Experimental Analysis of Corn Stalks Chopped Effect Based on Different Snapping Rollers Feed Angles%喂入角对玉米茎秆切碎效果的试验分析

    Institute of Scientific and Technical Information of China (English)

    魏元振; 李其昀; 尹成海; 刘庆国; 孙雪峰; 曹树红

    2016-01-01

    Designed structures and setting up a cut stems harvesting corn test bench of corn plant feeding angle adjusta -ble .In order to find out the best level of every factor and the primary and secondary factors in four factors by range analy -sis of orthogonal test .The results of orthogonal test showed that the optimal of cutting effect of corn on variable feeding angle cutting corn stalks device operation combination was:ear-snapping roll angle 90°, advance speed 0.7m/s, snap-ping roller rotate speed 1000 r/min and knife shaft rotate speed 1980 r/min in a given level .Feeding angles have a signif-icant impact to the cutting effect of corn plants on variable feeding angle cutting corn stalks device .The study reaches that when feeding angle is 0 °, the chopped pass rate is about 72 .8%, because the maize plants movement happened 90 °steering , producing dynamic impact .And when feeding angle is 30 °, the chopped pass rate is about 87 .6%.This re-sult is caused by as follows this reason that the transmission process on feeding channel is intermittent caused that the feeding of maize plants is not unimpeded and affects the chopped pass rate .And when feeding angle is 90 °, the chopped pass rate is approximately 97 .6% because snapping rolls rotation is conducive to maize plants feeding and being chopped .The corn plant function relation between the shear movements is established by analyzing the movement of maize plants in the cutting stems harvesting corn test bench of corn plant feeding angle adjustable .The final motion mathematical model between the shear movements is deduced through the two angular adjustments .The motion trajectory of maize plants on the cut stems harvesting corn test bench of corn plant feeding angle adjustable was clearly captured and tracked by the high speed photography technology during the experiments .Finally , the drawing was plotted and the test data was fitted by MATLAB software to reveal the essence of chopped effects on the feed

  4. Reshaping of the maize transcriptome by domestication.

    Science.gov (United States)

    Swanson-Wagner, Ruth; Briskine, Roman; Schaefer, Robert; Hufford, Matthew B; Ross-Ibarra, Jeffrey; Myers, Chad L; Tiffin, Peter; Springer, Nathan M

    2012-07-17

    Through domestication, humans have substantially altered the morphology of Zea mays ssp. parviglumis (teosinte) into the currently recognizable maize. This system serves as a model for studying adaptation, genome evolution, and the genetics and evolution of complex traits. To examine how domestication has reshaped the transcriptome of maize seedlings, we used expression profiling of 18,242 genes for 38 diverse maize genotypes and 24 teosinte genotypes. We detected evidence for more than 600 genes having significantly different expression levels in maize compared with teosinte. Moreover, more than 1,100 genes showed significantly altered coexpression profiles, reflective of substantial rewiring of the transcriptome since domestication. The genes with altered expression show a significant enrichment for genes previously identified through population genetic analyses as likely targets of selection during maize domestication and improvement; 46 genes previously identified as putative targets of selection also exhibit altered expression levels and coexpression relationships. We also identified 45 genes with altered, primarily higher, expression in inbred relative to outcrossed teosinte. These genes are enriched for functions related to biotic stress and may reflect responses to the effects of inbreeding. This study not only documents alterations in the maize transcriptome following domestication, identifying several genes that may have contributed to the evolution of maize, but highlights the complementary information that can be gained by combining gene expression with population genetic analyses.

  5. Nutrient absorbtion of weeds in maize.

    Science.gov (United States)

    Lehoczky, E; Kismányoky, A; Nagy, P; Németh, T

    2008-01-01

    Our study was carried out in Hungary at Keszthely, in 2007. The effect of different cultivation methods: no-till drill, disk tillage, conventional tillage (ploughing) and five increasing N doses were studied on the weediness. The bi-factorial trial was arranged in split plot design with four replications. Crop rotation: winter wheat-winter wheat-maize-maize. The seeding of maize was 23rd of April in 2007. The weed survey was made with Balázs-Ujvárosi coenological method on the 17th of May. In the experiment were found 21 weed species. We collected all plants of every weed species by plots. The sample area was 1 m2. Furthermore five maize plants per plot were sampled on the 22nd of May. Maize was at 3-4 leaves stage. For reason of competition studies no herbicides were applied on sampling sites. The aerial parts of weeds and maize plants were collected, and the fresh and dry matter weight was measured. We analyzed in detail, the occurrence of weed species, and the biomass production of weeds in comparison with maize. The effect of different cultivation methods markedly demonstrated the weed cover, the number of perennial and annual weeds and the number of occurring weed species.

  6. Lightweight concrete masonry units based on processed granulate of corn cob as aggregate

    Directory of Open Access Journals (Sweden)

    Faustino, J.

    2015-06-01

    Full Text Available A research work was performed in order to assess the potential application of processed granulate of corn cob (PCC as an alternative lightweight aggregate for the manufacturing process of lightweight concrete masonry units (CMU. Therefore, CMU-PCC were prepared in a factory using a typical lightweight concrete mixture for non-structural purposes. Additionally, lightweight concrete masonry units based on a currently applied lightweight aggregate such as expanded clay (CMU-EC were also manufactured. An experimental work allowed achieving a set of results that suggest that the proposed building product presents interesting material properties within the masonry wall context. Therefore, this unit is promising for both interior and exterior applications. This conclusion is even more relevant considering that corn cob is an agricultural waste product.En este trabajo de investigación se evaluó la posible aplicación de granulado procesado de la mazorca de maiz como un árido ligero alternativo en el proceso de fabricación de unidades de mampostería de hormigón ligero. Con esta finalidad, se prepararon en una fábrica diversas unidades de mampostería no estructural con granulado procesado de la mazorca de maiz. Además, se fabricaran unidades de mampostería estándar de peso ligero basado en agregados de arcilla expandida. Este trabajo experimental permitió lograr un conjunto de resultados que sugieren que el producto de construcción propuesto presenta interesantes propiedades materiales en el contexto de la pared de mampostería. Por lo tanto, esta solución es prometedora tanto para aplicaciones interiores y exteriores. Esta conclusión es aún más relevante teniendo en cuenta que la mazorca de maíz es un producto de desecho agrícola.

  7. Development of RNAi methods for Peregrinus maidis, the corn planthopper.

    Science.gov (United States)

    Yao, Jianxiu; Rotenberg, Dorith; Afsharifar, Alireza; Barandoc-Alviar, Karen; Whitfield, Anna E

    2013-01-01

    The corn planthopper, Peregrinus maidis, is a major pest of agronomically-important crops. Peregrinus maidis has a large geographical distribution and transmits Maize mosaic rhabdovirus (MMV) and Maize stripe tenuivirus (MSpV). The objective of this study was to develop effective RNAi methods for P. maidis. Vacuolar-ATPase (V-ATPase) is an essential enzyme for hydrolysis of ATP and for transport of protons out of cells thereby maintaining membrane ion balance, and it has been demonstrated to be an efficacious target for RNAi in other insects. In this study, two genes encoding subunits of P. maidis V-ATPase (V-ATPase B and V-ATPase D) were chosen as RNAi target genes. The open reading frames of V-ATPase B and D were generated and used for constructing dsRNA fragments. Experiments were conducted using oral delivery and microinjection of V-ATPase B and V-ATPase D dsRNA to investigate the effectiveness of RNAi in P. maidis. Real-time quantitative reverse transcriptase-PCR (qRT-PCR) analysis indicated that microinjection of V-ATPase dsRNA led to a minimum reduction of 27-fold in the normalized abundance of V-ATPase transcripts two days post injection, while ingestion of dsRNA resulted in a two-fold reduction after six days of feeding. While both methods of dsRNA delivery resulted in knockdown of target transcripts, the injection method was more rapid and effective. The reduction in V-ATPase transcript abundance resulted in observable phenotypes. Specifically, the development of nymphs injected with 200 ng of either V-ATPase B or D dsRNA was impaired, resulting in higher mortality and lower fecundity than control insects injected with GFP dsRNA. Microscopic examination of these insects revealed that female reproductive organs did not develop normally. The successful development of RNAi in P. maidis to target specific genes will enable the development of new insect control strategies and functional analysis of vital genes and genes associated with interactions between P

  8. Cytotoxicity of Ustilago maydis isolated from maize

    Directory of Open Access Journals (Sweden)

    Magdalena Twarużek

    2013-03-01

    Full Text Available The main pathogen of maize are fungi of the genus Fusarium. Besides phytopathogenic Fusarium, Ustilago maydis is another fungal genus affecting maize yields, causing lesions, known as smut. The objective of the study was evaluation of the cytotoxicity of Ustilago maydis isolated from maize. Nine Ustilago maydis strains were selected to a detailed evaluation of their cytotoxicity using a 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT test. Ustilago maydis strains showed medium and high cytotoxicity compared to control. High levels of cytotoxicity of Ustilago maydis may be indicative of their toxigenic potential.

  9. Hardness methods for testing maize kernels.

    Science.gov (United States)

    Fox, Glen; Manley, Marena

    2009-07-08

    Maize is a highly important crop to many countries around the world, through the sale of the maize crop to domestic processors and subsequent production of maize products and also provides a staple food to subsistance farms in undeveloped countries. In many countries, there have been long-term research efforts to develop a suitable hardness method that could assist the maize industry in improving efficiency in processing as well as possibly providing a quality specification for maize growers, which could attract a premium. This paper focuses specifically on hardness and reviews a number of methodologies as well as important biochemical aspects of maize that contribute to maize hardness used internationally. Numerous foods are produced from maize, and hardness has been described as having an impact on food quality. However, the basis of hardness and measurement of hardness are very general and would apply to any use of maize from any country. From the published literature, it would appear that one of the simpler methods used to measure hardness is a grinding step followed by a sieving step, using multiple sieve sizes. This would allow the range in hardness within a sample as well as average particle size and/or coarse/fine ratio to be calculated. Any of these parameters could easily be used as reference values for the development of near-infrared (NIR) spectroscopy calibrations. The development of precise NIR calibrations will provide an excellent tool for breeders, handlers, and processors to deliver specific cultivars in the case of growers and bulk loads in the case of handlers, thereby ensuring the most efficient use of maize by domestic and international processors. This paper also considers previous research describing the biochemical aspects of maize that have been related to maize hardness. Both starch and protein affect hardness, with most research focusing on the storage proteins (zeins). Both the content and composition of the zein fractions affect

  10. Assessment of maize stem borer damage on hybrid maize varieties in Chitwan, Nepal

    OpenAIRE

    Achhami, Buddhi Bahadur; Santa Bahadur BK; Bhandari, GhanaShyam

    2015-01-01

    Maize is the second most important cereal crop in Nepal. However, national figure of grain production still remains below than the world's average grain production per unit area. Thus, this experiment was designed to determine the suitable time of maize planting, and to assess the peak period of one of the major insects, maize stem borer, in Chitwan condition. The results showed that plant damage percentage as per the maize planting month varies significantly, and...

  11. Heterosis of maize photosynthetic performance

    Institute of Scientific and Technical Information of China (English)

    LI Xia; DING Zaisong; LI Lianlu; WANG Meiyun; ZHAO Ming

    2007-01-01

    Four maize inbred lines with different photosyn-thetic rates and their two hybrids were used as test materials,and the diurnal variations of their photosynthesis parameters in the silking stage were measured to study the heterosis of photosynthetic performance.Results showed that net photo-synthetic rate (In),transpiration rate (Tr) and stomatal conductance (Gs) all presented an obvious single-peaked curve in a day,with the peak values occurring at 10:00-12:00,12:00,10:00-12:00 a.m.,respectively,while water use efficiency (WUE) had a"V"type variant trend,with the lowest value appearing at 12:00.The diurnal variation of Pn and Tr was correlated markedly with Gs,suggesting that Gs played an important role in regulating the diurnal variation of Pn and Tr,and Pn,Tr and Gs had a higher heterosis in the afternoon than in the morning,while the WUE was in reverse,indicating that maize hybrid had higher resistance to the high temperature and dehydration in the afternoon,which provided a new path to select varieties with a high net photosynthetic rate.

  12. Effects of 90-Day Feeding of Transgenic Maize BT799 on the Reproductive System in Male Wistar Rats

    Directory of Open Access Journals (Sweden)

    Qian-ying Guo

    2015-12-01

    Full Text Available BT799 is a genetically modified (GM maize plant that expresses the Cry1Ac gene from Bacillus thuringiensis (Bt. The Cry1Ac gene was introduced into maize line Zhen58 to encode the Bt crystal protein and thus produce insect-resistant maize BT799. Expression of Bt protein in planta confers resistance to Lepidopteran pests and corn rootworms. The present study was designed to investigate any potential effects of BT799 on the reproductive system of male rats and evaluate the nutritional value of diets containing BT799 maize grain in a 90-day subchronic rodent feeding study. Male Wistar rats were fed with diets containing BT799 maize flours or made from its near isogenic control (Zhen58 at a concentration of 84.7%, nutritionally equal to the standard AIN-93G diet. Another blank control group of male rats were treated with commercial AIN-93G diet. No significant differences in body weight, hematology and serum chemistry results were observed between rats fed with the diets containing transgenic BT799, Zhen58 and the control in this 13-week feeding study. Results of serum hormone levels, sperm parameters and relative organ/body weights indicated no treatment-related side effects on the reproductive system of male rats. In addition, no diet-related changes were found in necropsy and histopathology examinations. Based on results of the current study, we did not find any differences in the parameters tested in our study of the reproductive system of male rats between BT799 and Zhen58 or the control.

  13. Rapidly induced chemical defenses in maize stems and their effects on short-term growth of Ostrinia nubilalis.

    Science.gov (United States)

    Dafoe, Nicole J; Huffaker, Alisa; Vaughan, Martha M; Duehl, Adrian J; Teal, Peter E; Schmelz, Eric A

    2011-09-01

    Plants damaged by insect herbivory often respond by inducing a suite of defenses that can negatively affect an insect's growth and fecundity. Ostrinia nubilalis (European corn borer, ECB) is one of the most devastating insect pests of maize, and in the current study, we examined the early biochemical changes that occur in maize stems in response to ECB herbivory and how these rapidly induced defenses influence the growth of ECB. We measured the quantities of known maize defense compounds, benzoxazinoids and the kauralexin class of diterpenoid phytoalexins. ECB herbivory resulted in decreased levels of the benzoxazinoid, 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one)-β-D-glucopyranose (DIMBOA-Glc), and a corresponding increase in 2-(2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one)-β-D-glucopyranose (HDMBOA-Glc). Total quantities of benzoxazinoids and kauralexins were increased as early as 24 h after the initiation of ECB feeding. The plant hormones, jasmonic acid (JA) and ethylene (ET), and the transcripts encoding their key biosynthetic enzymes also accumulated in response to ECB herbivory, consistent with a role in defense regulation. The combined pharmacological application of JA and the ET precursor, 1-aminocyclopropane-1-carboxylic acid to stem internode tissue likewise resulted in changes in benzoxazinoids similar to that observed with ECB damage. Despite the fact that maize actively mounts a defense response to ECB stem feeding, no differences in percent weight gain were observed between ECB larvae that fed upon non-wounded control tissues compared to tissues obtained from plants previously subjected to 24 h ECB stem herbivory. These rapid defense responses in maize stems do not appear to negatively impact ECB growth, thus suggesting that ECB have adapted to these induced biochemical changes.

  14. Risk Assessment of Genetically Engineered Maize Resistant to Diabrotica spp.: Influence on Above-Ground Arthropods in the Czech Republic.

    Directory of Open Access Journals (Sweden)

    Zdeňka Svobodová

    Full Text Available Transgenic maize MON88017, expressing the Cry3Bb1 toxin from Bacillus thuringiensis (Bt maize, confers resistance to corn rootworms (Diabrotica spp. and provides tolerance to the herbicide glyphosate. However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required. The MON88017 event was therefore evaluated under field conditions in Southern Bohemia in 2009-2011, to detect possible impacts on the above-ground arthropod species. The study compared MON88017, its near-isogenic non-Bt hybrid DK315 (treated or not treated with the soil insecticide Dursban 10G and two non-Bt reference hybrids (KIPOUS and PR38N86. Each hybrid was grown on five 0.5 ha plots distributed in a 14-ha field with a Latin square design. Semiquantitative ELISA was used to verify Cry3Bb1 toxin levels in the Bt maize. The species spectrum of non-target invertebrates changed during seasons and was affected by weather conditions. The thrips Frankliniella occidentalis was the most abundant species in all three successive years. The next most common species were aphids Rhopalosiphum padi and Metopolophium dirhodum. Frequently observed predators included Orius spp. and several species within the Coccinellidae. Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05. Multivariate analysis showed that the abundance and diversity of plant dwelling insects was similar in maize with the same genetic background, for both Bt (MON88017 and non-Bt (DK315 untreated or insecticide treated. KIPOUS and PR38N86 showed some differences in species abundance relative to the Bt maize and its near-isogenic hybrid. However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability.

  15. Risk Assessment of Genetically Engineered Maize Resistant to Diabrotica spp.: Influence on Above-Ground Arthropods in the Czech Republic

    Science.gov (United States)

    Svobodová, Zdeňka; Skoková Habuštová, Oxana; Hutchison, William D.; Hussein, Hany M.; Sehnal, František

    2015-01-01

    Transgenic maize MON88017, expressing the Cry3Bb1 toxin from Bacillus thuringiensis (Bt maize), confers resistance to corn rootworms (Diabrotica spp.) and provides tolerance to the herbicide glyphosate. However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required. The MON88017 event was therefore evaluated under field conditions in Southern Bohemia in 2009–2011, to detect possible impacts on the above-ground arthropod species. The study compared MON88017, its near-isogenic non-Bt hybrid DK315 (treated or not treated with the soil insecticide Dursban 10G) and two non-Bt reference hybrids (KIPOUS and PR38N86). Each hybrid was grown on five 0.5 ha plots distributed in a 14-ha field with a Latin square design. Semiquantitative ELISA was used to verify Cry3Bb1 toxin levels in the Bt maize. The species spectrum of non-target invertebrates changed during seasons and was affected by weather conditions. The thrips Frankliniella occidentalis was the most abundant species in all three successive years. The next most common species were aphids Rhopalosiphum padi and Metopolophium dirhodum. Frequently observed predators included Orius spp. and several species within the Coccinellidae. Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05). Multivariate analysis showed that the abundance and diversity of plant dwelling insects was similar in maize with the same genetic background, for both Bt (MON88017) and non-Bt (DK315) untreated or insecticide treated. KIPOUS and PR38N86 showed some differences in species abundance relative to the Bt maize and its near-isogenic hybrid. However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability. PMID:26083254

  16. VARIATION IN ADAPTIVE TRAITS OF CORN HYBRIDS FIRST GENERATION (GENOTYPE) UNDER THE INFLUENCE OF GROWTH REGULATOR Варьирование адаптивных свойств гибридов кукурузы первого поколения (генотипов) под влиянием регулятора роста

    OpenAIRE

    2012-01-01

    The article reviews the results of studying the adaptive potential of hybrids of corn maturity of different groups in the Central Caucasus. It discusses the variation of adaptive traits of maize hybrids (ecological plasticity and stability of the manifestations of agronomic traits), as well as stabilizing the yield of corn on an annual basis in the processing of seed preparation "TMTD-plus", which contains in its composition Krezatsin growth regulator

  17. Detection limits of the strip test and PCR for genetically modified corn in Brazil.

    Science.gov (United States)

    Nascimento, V E; Von Pinho, É V R; Von Pinho, R G; do Nascimento, A D

    2012-08-16

    Brazilian legislation establishes a labeling limit for products that contain more than 1% material from genetically modified organisms (GMOs). We assessed the sensitivity of the lateral flow strip test in detection of the GMO corn varieties Bt11 and MON810 and the specificity and sensitivity of PCR techniques for their detection. For the strip test, the GMO seeds were mixed with conventional seeds at levels of 0.2, 0.4 and 0.8% for Bt11, and 0.4, 0.8 and 1.6% for MON810. Three different methodologies were assessed and whole seeds, their endosperm and embryonic axis were used. For the PCR technique, the GMO seeds of each of the two varieties were mixed with conventional seeds at levels of 20, 10, 5, 2, 1, and 0.5%. The seeds were ground and the DNA extracted. For detection of the GMO material, specific primers were used for MON810 and Bt11 and maize zein as an endogenous control. The sensitivity of the strip test varied for both maize varieties and methodologies. The test was positive for Bt11 only at 0.8%, in contrast with the detection limit of 0.4% indicated by the manufacturer. In the multiplex PCR, the primers proved to be specific for the different varieties. These varieties were detected in samples with one GMO seed in 100. Thus, this technique proved to be efficient in detecting contaminations equal to or greater than 1%.

  18. 玉米生长动态模拟仿真系统的研究%Research on Dynamic Simulation System of Maize Growth

    Institute of Scientific and Technical Information of China (English)

    朱春娆; 陈桂芬; 苏恒强

    2011-01-01

    Dynamic simulation system of maize growth was developed by the physiological and ecological model, morphological structure model, computer science and virtual reality technology, to improve the level of precise operation of corn production. The computer graphics algorithms, virtual reality technology, animation design and information integration technology were applied to maize production by this system. Establishment of dynamic simulation system of maize growth was conducive to raise level of precise operation in corn production. The system also could assist the relevant production research and testing, to reduce cost and improve efficiency.%为了加快提高玉米生产精准化作业的水平,利用玉米生理生态模型、形态结构模型、计算机和虚拟现实技术,研制开发了玉米生长动态模拟仿真系统,将计算机图形学算法、虚拟现实技术、动画设计技术以及信息集成技术应用到玉米生产领域.建立的生长动态模拟仿真系统有利于玉米精准化作业水平的提高,能够协助相关的生产研究和试验,达到降低成本、提高效率的目的.

  19. Increased Survival of Western Corn Rootworm on Transgenic Corn Within Three Generations of Onplant Greenhouse Selection

    Science.gov (United States)

    The agricultural industry has adopted a high dose/refuge strategy as a means of delaying the onset of insect resistance to transgenic crops. Recently, Bt corn products developed for control of western corn rootworm (WCR), Diabrotica virgifera virgifera, have been introduced with less than high-dose...

  20. Use of Spectral Vegetation Indices for Detection of European Corn Borer Infestation in Iowa Corn Plots

    Science.gov (United States)

    Recently, corn grown for grain in the United States has increased from 28 million ha in 2006 to more than 35 million ha in 2007 with a production value of over $52 billion dollars. Transgenic corn expressing the plant incorporated protectant Bacillus thuringiensis toxin represen...

  1. Corn Snake Genetics: Students Learn about the Fundamentals of Mendelism by Studying Corn Snakes

    Science.gov (United States)

    King, Kristin

    2004-01-01

    In an attempt to generate student enthusiasm on the subject of genetics, the author developed a Punnett square activity centered on the genetics of corn snakes to teach students about Mendelism and genetic diversity. As they began the activity, however, some unexpected twists occurred that allowed for investigation into corn snake anatomy and…

  2. Environmental effect of conventional and GM crops of cotton (Gossypium hirsutum L. and corn (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Chaparro Giraldo Alejandro

    2011-12-01

    Full Text Available

    In the corn belt of Valle de San Juan and in the cotton zone of El Espinal, municipalities in the department of Tolima (Colombia, 10 conventional corn producers, 10 producers of genetically modified corn, five producers of conventional cotton and 15 producers of transgenic cotton were surveyed in the first half of 2009 to contrast the differences in the environmental impact associated with use of insecticides and herbicides, which were evaluated by estimating the environmental index quotient-EIQ. In the case of maize, an EIQ of 42 was found in the conventional type, while transgenic technology had an EIQ of 3.03. In the cultivation of cotton, an EIQ of 263.59 was found for the conventional type while for transgenic technology this value varied between 335.75 (Nuopal BG/RR and 324.79 (DP 455 BG/RR. These data showed a lower environmental impact using GM technology in the cultivation of maize when compared to the conventional counterpart, in connection with the use of insecticides and herbicides, in the context of time, space and genotypic analysis. This effect was not observed in the case of cotton, where environmental impacts were similar.

  3. EFFECT OF DIFFERENT TRAPS ON CAPTURES OF ADULT CORN ROOTWORM BEETLES (Diabrotica virgifera virgifera LeConte IN EAST SLAVONIA

    Directory of Open Access Journals (Sweden)

    Marija Ivezić

    2002-12-01

    Full Text Available In 1995 the corn rootworm (Diabrotica virgifera virgifera LeConte was detected for the first time in east Slavonia, Croatia. Its expansion to the west part has been very fast and from year to year populations of the pest are higher, especially when corn is planted after corn. The aim of this investigation was to find the best trap for monitoring Diabrotica. The studies were conducted by using three types of traps: the USA Trece lure trap, Multigard yellow sticky trap and Hungarian pheromone trap. The treatments were replicated four times in a maize field located in the east of Croatia, very near to the Yugoslavia border. The traps were placed in the field on the 23rd of June and the experiment continued until the 15th of September. Pheromone and Multigard sticky traps were replaced with new ones each month. Traps were positioned 60 m apart within a row and 70 m apart across maize rows. Silking occurred from 30 June to 8 August (exposed silk thoroughly brown and dry. This year was extremely dry, and the first beetles were noticed on the 15th of June. It was 20 days earlier than in 1999. Adult rootworm beetles were removed from the traps once weekly. Pheromone traps captured the most beetles (2263, Multigard sticky traps caught the second most (214 and lure traps the fewest (182 for the whole period. After the replacement of pheromone and Multigard traps, the capture of beetles increased. Of the total number of beetles caught, 85.10% was caught by the pheromone traps, 8.05% by the Multigard sticky traps and 6.85% by the lure traps. Due to this year’s dryness (50% less rainfall than the 20 year average, the investigation should be continued in the future to get more precise results.

  4. Microbial communities associated with the larval gut and eggs of the Western corn rootworm.

    Directory of Open Access Journals (Sweden)

    Flavia Dematheis

    Full Text Available BACKGROUND: The western corn rootworm (WCR is one of the economically most important pests of maize. A better understanding of microbial communities associated with guts and eggs of the WCR is required in order to develop new pest control strategies, and to assess the potential role of the WCR in the dissemination of microorganisms, e.g., mycotoxin-producing fungi. METHODOLOGY/PRINCIPAL FINDINGS: Total community (TC DNA was extracted from maize rhizosphere, WCR eggs, and guts of larvae feeding on maize roots grown in three different soil types. Denaturing gradient gel electrophoresis (DGGE and sequencing of 16S rRNA gene and ITS fragments, PCR-amplified from TC DNA, were used to investigate the fungal and bacterial communities, respectively. Microorganisms in the WCR gut were not influenced by the soil type. Dominant fungal populations in the gut were affiliated to Fusarium spp., while Wolbachia was the most abundant bacterial genus. Identical ribosomal sequences from gut and egg samples confirmed a transovarial transmission of Wolbachia sp. Betaproteobacterial DGGE indicated a stable association of Herbaspirillum sp. with the WCR gut. Dominant egg-associated microorganisms were the bacterium Wolbachia sp. and the fungus Mortierella gamsii. CONCLUSION/SIGNIFICANCE: The soil type-independent composition of the microbial communities in the WCR gut and the dominance of only a few microbial populations suggested either a highly selective environment in the gut lumen or a high abundance of intracellular microorganisms in the gut epithelium. The dominance of Fusarium species in the guts indicated WCR larvae as vectors of mycotoxin-producing fungi. The stable association of Herbaspirillum sp. with WCR gut systems and the absence of corresponding sequences in WCR eggs suggested that this bacterium was postnatally acquired from the environment. The present study provided new insights into the microbial communities associated with larval guts and eggs of

  5. Evaluation of maize yield in an on-farm maize-soybean and maize-Lablab crop rotation systems in the Northern Guinea Savanna of Nigeria.

    Science.gov (United States)

    Okogun, J A; Sanginga, N; Abaidoo, R C

    2007-11-01

    An attempt was made to solving the problem of shortfall of fertilizer to maize production in the Northern Guinea Savanna (NGS) of Nigeria by harnessing the potentials of legume/cereal crop rotation in on-farm trials. The yield of maize that succeeded two soybean varieties and Lablab in a two-cycle of soybean/maize and Lablab/maize crop rotation in NGS Nigeria was assessed in researcher-managed and farmer-managed plots. Though maize that followed the soybean received between 5 kg N ha(-1) from improved soybean variety (TGx 1448-2E) and 17 kg N ha(-1) from farmer soybean variety (Samsoy-2) as N balance, this did not significantly (p = 0.05) affect the maize yields. The soybean shed 90-100% of its leaves at physiological maturity which resulted in about 110 kg N ha(-1) N uptake. This source of N might be one of the factors responsible for the increase in maize yield that followed soybean (20 to 24%) compared with continuous maize yield plot. Maize yield in previous Lablab plot was significantly (p = 0.05) higher than in all other treatments. Maize yield in farmer-managed plot ranged between 0.13 and 4.53 t ha(-1), maize yield in researcher-managed plot was over 200% higher than maize yield in farmer-managed plot because of poor crop management on the part of the farmer.

  6. Study of effects of Bt maize (Zea mays) events on Lepidoptera Ostrinia nubilalis, Sesamia nonagrioidesin southwestern France.

    Science.gov (United States)

    Folcher, L; Eychenne, N; Weissenberger, A; Jarry, M; Regnault-Roger, C; Delos, M

    2006-01-01

    Crops of maize (Zea mays L.) were conducted in southwestern France with GMO (Genetic Modified Organism) vs isogenetic varieties in order to verify the control of European Corn Borer (ECB) Ostrinia nubilalis (Hübner) and the Corn Stalk Borer (CBS) Sesamia nonagrioides (Lefevbre) by GMO in field conditions. The bioassays were carried out in 1998 and 1999 before moratorium, then in 2005. Experiments involved respectively 18, 12 and 19 fields cultivated with Furio/Furio cb (GMO), Cecilia/ Elgina (GMO) and PR33P66/PR33P67 (GMO) varieties. These transgenic events expressed Cry1A(b) protein (Bt maize). Plants were noted for insect infestation assessment (number of larvae in stalks and ears per plant). Statistical tests used t-test on couple of plots. Results showed a significant difference in the density of both ECB and CBS between control and the two transgenic events. The two transgenic events acted differently. The control of the two Bt events on the two pests were differentiated and discussed. These experiments underlined the importance of field evaluation for testing real effects of transgenic events on crop according the environmental context.

  7. Successfully introduce maize DNA fragments into rice

    Institute of Scientific and Technical Information of China (English)

    WANGKaizhi

    1994-01-01

    The maize DNA fragments was successfully incorporated into rice by Associate Prof WAN Wenju's research team at Hunan Agricultural College, Changsha, China. The new gene transferring rice is named Genetic Engineered Rice (GER) line.

  8. 适于机械化粒收玉米新品种育种策略探讨%Discussion on Breeding Strategy for New Maize Varieties Suitable for Mechanized Grain Harvest

    Institute of Scientific and Technical Information of China (English)

    张成华; 方志军; 齐世军; 陈凯; 丁照华; 杨菲; 张发军

    2016-01-01

    With the development of maize production in Shandong Province, maize harvest mechanization level is higher and higher.From ar-tificial harvesting to mechanical spike harvesting, and then to mechanical grain harvesting, is an important change in the harvest of corn.The transformation of harvesting methods put forward new requirements for maize varieties selection and breeding.According to the development status of corn harvest mechanization in Shandong Province, the maize inbred lines and hybrid breeding targets which were suitable for mecha-nized grain harvest were discussed, so as to provide reference for breeding work.%随着山东省玉米生产的发展,玉米机械化收获水平越来越高。从人工收获到机械收穗,再到机械粒收,是玉米收获的重要变革。收获方式的转变对玉米品种选育提出了新的要求,结合山东省玉米机械化收获发展状况,探讨了适宜机械化粒收的玉米自交系和杂交种选育目标,以期为育种工作提供参考。

  9. GROWTH AND PRODUCTION OF CORN IN FUNCTION OF THE APPLICATION OF CATTLE MANURE AND BIOFERTILIZER

    Directory of Open Access Journals (Sweden)

    Fabricio da Silva Oliveira

    2011-07-01

    Full Text Available We studied the effect of 9 quantities of cattle manure (Q1 = 0 kg / pit, Q2= 1.0 kg / hill, Q3 = 2.0 kg / hill, Q4 = 3.0 kg/hill, Q5 = 4,0kg/pit, Q6=5.0kg/hill, Q7 =6.0kg/hill,; Q8 =7.0kg/hill, Q9 = 8.0kg/hill and 5 doses of biofertilizer (D1 = 0 mL / plant / time, D2 = 30 mL / plant / time, D3 = 60 mL / plant / time, D4 = 90 mL / plant / time, D5 = 120 mL / plant / time, growth and the productivity of maize. The experimental design was to randomized blocks, 45 treatments in a factorial scheme 9x5, with 4 replications, and planted 2 plants per plot in each repetition, totaling 360 experimental plants. The growth and production of corn were not affected significantly when it was applied to 8 kg of cattle manure pit in the foundation of fertilization, growth and production of corn were not affected significantly when applications were made 5 of biofertilizer in fertilization coverage in dosages up to 120mL/plant/time.

  10. Metabolite variation in hybrid corn grain from a large-scale multisite study

    Institute of Scientific and Technical Information of China (English)

    Mingjie Chen; R Shyama Prasad Rao; Yiming Zhang; Cathy Zhong; Jay J Thelen

    2016-01-01

    Metabolite composition is strongly affected by genotype, environment, and interactions between genotype and environment, although the extent of variation caused by these factors may depend upon the type of metabolite. To characterize the complexity of genotype, environment, and their interaction in hybrid seeds, 50 genetically diverse non-genetically modified (GM) maize hybrids were grown in six geographically diverse locations in North America. Polar metabolites from 553 harvested corn grain samples were isolated and analyzed by gas chromatography–mass spectrometry and 45 metabolites detected in all samples were used to generate a data matrix for statistical analysis. There was moderate variation among biological replicates and across genotypes and test sites. The genotype effects were detected by univariate and Hierarchical clustering analyses (HCA) when environmental effects were excluded. Overall, environment exerted larger effects than genotype, and polar metabolite accumulation showed a geographic effect. We conclude that it is possible to increase seed polar metabolite content in hybrid corn by selection of appropriate inbred lines and growing regions.

  11. INFLUENCE OF SILAGE ADDITIVES ON FERMENTATION OF HIGH MOISTURE CRIMPED CORN

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    Branislav Gálik

    2009-03-01

    Full Text Available The objective of this work was to find influence of different silage additives on silages fermentation and nutritional value made from high moisture crimped corn, which were conserved in semi experimental conditions. Three variants were examined, untreated control (C, and two experimental variants conserved by biological (variant A and chemical (variant B additives. The maize crimped corn was hermetically filled into plastic bins with the capacity 50 dm3. In silage conserved by additives was lower content of crude fibre (significantly in both experimental variants and higher content of nitrogen free extract, starch and total sugars (significantly in variant A established. In silages form both experimental variants we found significantly lower content of lactic acid. The highest concent we detected in silage conserved without additives. In silage conserved by biological inoculant we found lower content of acetic acid and higher content of butyric acid, but their content was generally very low. Additives used in the experiment decreased content of amonia (0.074 g.kg-1 in variant A and 0.095 g.kg-1 of dry matter in variant B and alcohols too.

  12. Metabolite variation in hybrid corn grain from a large-scale multisite study

    Directory of Open Access Journals (Sweden)

    Mingjie Chen

    2016-06-01

    Full Text Available Metabolite composition is strongly affected by genotype, environment, and interactions between genotype and environment, although the extent of variation caused by these factors may depend upon the type of metabolite. To characterize the complexity of genotype, environment, and their interaction in hybrid seeds, 50 genetically diverse non-genetically modified (GM maize hybrids were grown in six geographically diverse locations in North America. Polar metabolites from 553 harvested corn grain samples were isolated and analyzed by gas chromatography–mass spectrometry and 45 metabolites detected in all samples were used to generate a data matrix for statistical analysis. There was moderate variation among biological replicates and across genotypes and test sites. The genotype effects were detected by univariate and Hierarchical clustering analyses (HCA when environmental effects were excluded. Overall, environment exerted larger effects than genotype, and polar metabolite accumulation showed a geographic effect. We conclude that it is possible to increase seed polar metabolite content in hybrid corn by selection of appropriate inbred lines and growing regions.

  13. [Effects of nitrogen management on maize nitrogen utilization and residual nitrate nitrogen in soil under maize/soybean and maize/sweet potato relay strip intercropping systems].

    Science.gov (United States)

    Wang, Xiao-Chun; Yang, Wen-Yu; Deng, Xiao-Yan; Zhang, Qun; Yong, Tai-Wen; Liu, Wei-Guo; Yang, Feng; Mao, Shu-Ming

    2014-10-01

    A large amount of nitrogen (N) fertilizers poured into the fields severely pollute the environment. Reasonable application of N fertilizer has always been the research hotpot. The effects of N management on maize N utilization and residual nitrate N in soil under maize/soybean and maize/ sweet potato relay strip intercropping systems were reported in a field experiment in southwest China. It was found that maize N accumulation, N harvest index, N absorption efficiency, N contribution proportion after the anthesis stage in maize/soybean relay strip intercropping were increased by 6.1%, 5.4%, 4.3%, and 15.1% than under maize/sweet potato with an increase of 22.6% for maize yield after sustainable growing of maize/soybean intercropping system. Nitrate N accumulation in the 0-60 cm soil layer was 12.9% higher under maize/soybean intercropping than under maize/sweet potato intercropping. However, nitrate N concentration in the 60-120 cm soil layer when intercropped with soybean decreased by 10.3% than when intercropped with sweet potato, indicating a decrease of N leaching loss. Increasing of N application rate enhanced N accumulation of maize and decreased N use efficiency and significantly increased nitrate concentration in the soil profile except in the 60-100 cm soil layer, where no significant difference was observed with nitrogen application rate at 0 to 270 kg · hm(-2). Further application of N fertilizer significantly enhanced nitrate leaching loss. Postponing N application increased nitrate accumulation in the 60-100 cm soil layer. The results suggested that N application rates and ratio of base to top dressing had different influences on maize N concentration and nitrate N between maize/soybean and maize/sweet potato intercropping. Maize N concentration in the late growing stage, N harvest index and N use efficiency under maize/soybean intercropping increased (with N application rate at 180-270 kg · hm(-2) and ratio of base to top dressing = 3:2:5) and

  14. 新民市玉米机械化收获发展剖析%Analysis of Corn Harvest Mechanization Development in Xinmin City

    Institute of Scientific and Technical Information of China (English)

    陶竹林

    2014-01-01

    This article examines the present state of development in Xinmin agricultural mechanization and maize harvest rice mecha-nization. The author expounds on the necessity of developing maize harvest mechanization and its roles, probes into the main problems in the development of the corn harvest mechanization in existence, and puts forward specific development proposals.%在介绍新民市农业机械化及玉收获米机械化发展现状的基础上,重点介绍发展玉米收获机械化的必要性及作用,探讨全市玉米机械化收获发展中存的主要问题,并提出有针对性的发展建议。

  15. Assessing white maize resistance to fumonisin contamination

    OpenAIRE

    Cao Caamaño, Ana; Butrón Gómez, Ana María; Ramos, Antonio J.; Marín, Sonia; Souto, Carlos; Santiago Carabelos, Rogelio

    2014-01-01

    Genetic improvement is an emerging method to reduce the levels of fumonisin (FB) contamination in maize, but breeding advances depend on the development of suitable methods to accurately assess the performance of different cultivars. Our study focused on characterizing a local isolate of Fusarium verticillioides; comparing artificial inoculation techniques with this isolate (injection into kernels and down the silk channel); and assessing white maize resistance under artificial vs. natural in...

  16. AVALIAÇÃO DE CULTIVARES DE MILHO PARA O PROCESSAMENTO DE PAMONHA BEHAVIOR OF MAIZE CULTIVARS IN THE INDUSTRIAL PRODUCTION OF "PAMONHA"

    Directory of Open Access Journals (Sweden)

    Henriqueta Merçon Vieira Rolim

    2007-09-01

    Full Text Available

    Com o objetivo de avaliar o potencial de vinte genótipos de milho, quanto ao processamento de pamonha, procedeu-se à análise das características: peso de espigas com palha, peso de espigas sem palha, peso de massa, peso de quirela, peso de palha e profundidade de grão sob três condições (processado no dia da colheita ou após sete dias de armazenamento, com e sem refrigeração. Os resultados possibilitaram recomendar o híbrido AG 1051, como capaz de atender às exigências do agricultor e do mercado consumidor (pamonharias. Esse híbrido apresentou características desejáveis como produtividade (maior peso de espigas com palha e sem palha, alta capacidade de produção de massa e baixa produção de quirela.

    PALAVRAS-CHAVE: Milho verde; armazenamento; genótipos.

    The objective of this study was to evaluate the potential of twenty maize cultivars in the industrial processing of grated corn for pamonha. Data were obtained for ear weight with husks, ear weight with no husks, grated maize weight, grated maize residue weight, husk weight, and kernel depth under three storage conditions (processed on the day of harvest or after seven days of storage, with and without refrigeration. The results showed that the AG 1051 hybrid can be recommended for the industrial production of pamonha. This hybrid showed high yield and ear weight, with and without husks, the greatest grated corn production and reduced grated corn residue weight.

    KEY-WORDS: Zea mays; immature corn; green corn; corn storage.

  17. Effect of various soil salinity level on the antioxidant and physiological properties of corn plant (Zea mays

    Directory of Open Access Journals (Sweden)

    Ramin Ezzati

    2015-10-01

    Full Text Available To study the effect of various soil salinity levels on the antioxidant enzymes and physiological characteristics of corn as an indicator of salt stress resistance, present study was conducted in 2013 at Research lab of environmental, Faculty of Biological Sciences, University of Kharazmi, Iran. Furthermore, desert soils of Libya, India, Iraq and Karaj were used for testing the effect of soil salinity on the quantity of oxidant enzymes and various properties of maize plant. For providing favorable atmospheric conditions 33000 lux light and spray of perlite medium was used. Corn crop is highly sensitive to soil salinity so all the cultivation was carried out under conditions of soil EC lower than 20 ds/m and 8.5 pH. Results of the study revealed that the salt stress significantly affect the amount of antioxidant enzymes such as guaiacol peroxidase and malondialdehyde. Furthermore, it was reported that saline soil did not have any negative effect on the physiological characteristics and growth of corn plant growth.

  18. Corn (Hybrid BC666 Response to Supply Nutrients from Organic Nutrition (Azotobacter and Manure in Climate Conditions of Lorestan

    Directory of Open Access Journals (Sweden)

    M Beiranvadi

    2013-10-01

    Full Text Available Maize (Zea mays L. is one of the most important crops that used for food, feeding and industrial products and in Iran a developing cultivation. In order to study of corn s reactions (Hybrid BC666 to supply nutrients to organic nutrition (Bacteria and manure as summer sown in climate conditions of Lorestan. An experiment was conduct in Meteorology Station of Lorestan s agriculture research field during 2007 four planting densities as 75000,85000, 95000 and 105000 plant ha-1 in main plots and two amount manure (24 and 30 ton ha-1 set in subplot and application and non application Azotobacter chroococcum set in sub-sub plots , were arranged in a split split plot design as base of RCBD. The results indicated that plant densities significally affected Biological yield,grain yield and ear number in m2. Increase plant densities of 75000 to 105000 plant in per hectare caused 42% addition biological yield,65% addition grain yield and 42% raise ear number in m2 .eventually researches showed in climate conditions of Lorestan corn to be able as summer sown to enter rotation cycle. thus corn summer sown planting densities 105000 plant ha-1 is optimum density and application 24 ton ha-1 manure for supply plant nutrients to be enough. In rich soils with fertilizers, not available field for to show beneficial effects bacteria.

  19. Study of improvement of indices maize line to establish their position in hybrids

    Directory of Open Access Journals (Sweden)

    Oxana DIRZU-COCOS

    2015-12-01

    Full Text Available Corn (Zea mays L. crop that is grown on large areas - over 140 million hectares worldwide, and 400-500 thousand ha in Moldova due to production potential broad diversity of use as food for humans, animals, birds raw material for industrial processing. The upward trend in average yields achieved is largely attributed to the improvement of scientific programs. Select the line with the characters and traits that are transmitted hereditary hybrids and contribute to their performance, ensure progress in improvement. Therefore, the process of creating inbred lines associated with combining ability testing as a measure of productivity conferred hybrids, is significant research programs. Orientation purpose of improved maize hybrids to formulas and simple change to a superior capitalization heterosis effect and perfect uniformity of plant requires changes in methodology for the creation, evaluation and classification of inbred lines.

  20. Does Bt maize cultivation affect the non-target insect community in the agro ecosystem?

    Directory of Open Access Journals (Sweden)

    Daniela Chaves Resende

    2016-03-01

    Full Text Available ABSTRACT The cultivation of genetically modified crops in Brazil has led to the need to assess the impacts of this technology on non-target species. Under field conditions, the potential effect on insect biodiversity was evaluated by comparing a homogeneous corn field with conventional and transgenic maize, expressing different Bt proteins in seven counties of Minas Gerais, Brazil. The richness pattern of non-target insect species, secondary pests and natural enemies were observed. The results do not support the hypothesis that Bt protein affects insect biodiversity. The richness and diversity data of insects studied were dependent on the location and other factors, such as the use of insecticides, which may be a major factor where they are used.

  1. A developmentally regulated hydroxyproline-rich glycoprotein in maize pericarp cell walls. [Zea mays L

    Energy Technology Data Exchange (ETDEWEB)

    Hood, E.E.; Shen, Q.X.; Varner, J.E. (Washington Univ., St. Louis, MO (USA))

    1988-05-01

    We have studied the accumulation of peptidyl hydroxyproline in the pericarp of developing maize (Zea mays L., Golden cross Bantam sweet corn) kernels. Although this hydroxyproline accumulates throughout development, it is most soluble and its content per milligram dry weight greatest at midmaturation stages of development. Salt-soluble proteins containing this hydroxyproline from isolated cell walls of developing kernels were fractionated on a CsCl density gradient and on a Chromatofocusing column, resulting in the purification of an hydroxyproline-rich glycoprotein, PC-1. PC-1 is a basic protein of approximately 65 to 70 kilodaltons in molecular weight with an isoelectric point of at least 10.2 and a density of 1.38 to 1.39 in CsCl. Amino acid composition data indicate that it is rich in hydroxyproline, threonine, proline, lysine, and glycine. Its relation to dicot extension is discussed.

  2. Photoacoustic Determination of Non-radiative Relaxation Time of Absorbing Centers in Maize Seeds

    Science.gov (United States)

    Domínguez-Pacheco, A.; Hernández-Aguilar, C.; Cruz-Orea, A.

    2017-07-01

    Using non-destructive photothermal techniques, it is possible to characterize non-homogenous materials to obtain its optical and thermal properties through photoacoustic spectroscopy (PAS). In photoacoustic (PA) phenomena, there are transient states of thermal excitation, when samples absorb the incident light; these states manifest an excitation process that generates the PA signal, being in direct relation with the non-radiative relaxation times with the sample absorbent centers. The objective of this study was to determine the non-radiative relaxation times associated with different absorbent centers of corn seeds ( Zea mays L.), by using PAS. A frequency scan was done at different wavelengths (350 nm, 470 nm and 650 nm) in order to obtain the non-radiative relaxation times with different types of maize seeds.

  3. Maize leaf development under climate change scenarios

    Directory of Open Access Journals (Sweden)

    Nereu Augusto Streck

    2010-11-01

    Full Text Available The objective of this work was to simulate maize leaf development in climate change scenarios at Santa Maria, RS, Brazil, considering symmetric and asymmetric increases in air temperature. The model of Wang & Engel for leaf appearance rate (LAR, with genotype-specific coefficients for the maize variety BRS Missões, was used to simulate tip and expanded leaf accumulated number from emergence to flag leaf appearance and expansion, for nine emergence dates from August 15 to April 15. LAR model was run for each emergence date in 100-year climate scenarios: current climate, and +1, +2, +3, +4 and +5°C increase in mean air temperature, with symmetric and asymmetric increase in daily minimum and maximum air temperature. Maize crop failure due to frost decreased in elevated temperature scenarios, in the very early and very late emergence dates, indicating a lengthening in the maize growing season in warmer climates. The leaf development period in maize was shorter in elevated temperature scenarios, with greater shortening in asymmetric temperature increases, indicating that warmer nights accelerate vegetative development in maize.

  4. Climate Change and Maize Yield in Iowa.

    Science.gov (United States)

    Xu, Hong; Twine, Tracy E; Girvetz, Evan

    2016-01-01

    Climate is changing across the world, including the major maize-growing state of Iowa in the USA. To maintain crop yields, farmers will need a suite of adaptation strategies, and choice of strategy will depend on how the local to regional climate is expected to change. Here we predict how maize yield might change through the 21st century as compared with late 20th century yields across Iowa, USA, a region representing ideal climate and soils for maize production that contributes substantially to the global maize economy. To account for climate model uncertainty, we drive a dynamic ecosystem model with output from six climate models and two future climate forcing scenarios. Despite a wide range in the predicted amount of warming and change to summer precipitation, all simulations predict a decrease in maize yields from late 20th century to middle and late 21st century ranging from 15% to 50%. Linear regression of all models predicts a 6% state-averaged yield decrease for every 1°C increase in warm season average air temperature. When the influence of moisture stress on crop growth is removed from the model, yield decreases either remain the same or are reduced, depending on predicted changes in warm season precipitation. Our results suggest that even if maize were to receive all the water it needed, under the strongest climate forcing scenario yields will decline by 10-20% by the end of the 21st century.

  5. Pathogenicity of P. terrestris on Maize Seedlings

    Directory of Open Access Journals (Sweden)

    Jelena Lević

    2012-01-01

    Full Text Available Pathogenicity of P. terrestris was determined by the Knop’s medium slants method intest tubes. Isolates originated from the roots of maize (Zea mays L., barley (Hordeum vulgareL., Johnson grass (Sorghum halepense Pers., sorghum (Sorghum bicolour (L. Moench., garlic(Allium sativum L., onion (Allium cepa L., barnyard millet (Echinochloa crus-galli (L. P.Beauv.and green foxtail (Setaria viridis (L. P.B.. A fragment of a fungal colony, cultivated on PDA,was placed on the bottom of Knop’s medium slant in each test tube and then steriliseda maize seed was placed 2 cm away from the inoculum. After 21-day inoculation of seeds,the intensity of the development of symptoms on maize seedlings was estimated. The reddishor dark pigment on the root, mesocotyl and/or coleoptyl of seedlings was an indicatorfor the infection by the fungus under in vitro conditions. Based on the pathogenicity test,the isolates were classified into the following three groups: slightly (3 isolates, moderately(6 isolates and very pathogenic (6 isolates to maize seedlings. The obtained results showthat P. terrestris, originating from different hosts, can be a maize pathogen. These resultscan explain the high frequency and high incidence of this fungus on maize roots in Serbia.

  6. Effect of Plant Growth-Promoting Bacteria on Quantitative and Qualitative Yield of Forage Maize

    Directory of Open Access Journals (Sweden)

    R. Abasi

    2016-12-01

    Full Text Available This research was conducted to investigate the effect of growth-promoting bacteria on yield and some traits of maize. This experiment was performed as a factorial randomized complete block design with three replications at research farm of Islamic Azad University, Izeh branch, Iran. The experimental factors included four strains of plant growth-promoting bacteria: Pseudomonas fluorescent 169 (B1, Pseudomonas fluorescent 79 (B2, Pseudomonas putida 108 (B3, Pseudomonas putida 159 (B4 and without bacteria (control, B5 and two corn varieties namely sc 704 (A1 and Bolson (A2. Before planting corn seeds were soaked with liquid inoculation. Plant height, number of leaves per plant and ear, forage yield, dry matter and dry matter digestibility of leaf, stem and ear, crude protein and cell wall without hemicellulose in the leaf, stem and ear were evaluated. Analysis of variances showed that the effects of varieties, bacteria and their interaction on all traits were significant. Plant height, number of leaves per plant and ear, forage yield, dry matter and stem dry matter digestibility were higher in Bolson. Moreover, dry matter digestibility of leaf and ear, crude protein and cell wall without hemicellulose in the leaf, stem and ear of sc 704 were greater, compared to hybrid Bolson. Plant height, number of leaves per plant and ear, forage yield, dry matter and dry matter digestibility of stem and ear were greater in at the presence of fluorescent strains than those of putida strains. The results revealed that bacterial inoculation enhances the grain yield, yield components and quality of forage maize. Bolson seemed potent to outperform sc 704, though this proposition needs further examination in future field trials.

  7. Bats initiate vital agroecological interactions in corn.

    Science.gov (United States)

    Maine, Josiah J; Boyles, Justin G

    2015-10-01

    In agroecosystems worldwide, bats are voracious predators of crop pests and may provide services to farmers worth billions of U.S. dollars. However, such valuations make untested assumptions about the ecological effect of bats in agroecosystems. Specifically, estimates of the value of pest suppression services assume bats consume sufficient numbers of crop pests to affect impact pest reproduction and subsequent damage to crops. Corn is an essential crop for farmers, and is grown on more than 150 million hectares worldwide. Using large exclosures in corn fields, we show that bats exert sufficient pressure on crop pests to suppress larval densities and damage in this cosmopolitan crop. In addition, we show that bats suppress pest-associated fungal growth and mycotoxin in corn. We estimate the suppression of herbivory by insectivorous bats is worth more than 1 billion USD globally on this crop alone, and bats may further benefit farmers by indirectly suppressing pest-associated fungal growth and toxic compounds on corn. Bats face a variety of threats globally, but their relevance as predators of insects in ubiquitous corn-dominated landscapes underlines the economic and ecological importance of conserving biodiversity.

  8. Concentration and dissipation of chlorantraniliprole and thiamethoxam residues in maize straw, maize, and soil.

    Science.gov (United States)

    He, Min; Song, Dan; Jia, Hong C; Zheng, Yongquan

    2016-09-01

    To study the dissipation rates and final residual levels of chlorantraniliprole and thiamethoxam in maize straw, maize, and soil, two independent field trials were conducted during the 2014 cropping season in Beijing and Anhui Provinces of China. A 40% wettable powder (20% chlorantraniliprole + 20% thiamethoxam) was sprayed onto maize straw and soil at an application rate of 118 g of active ingredient per hectare (g a.i.ha(-1)). The residual concentrations were determined by ultra-high-performance liquid chromatography-tandem mass spectrometry. The chlorantraniliprole half-lives in maize straw and soil were 9.0-10.8 and 9.5-21.7 days, respectively. The thiamethoxam half-lives in maize straw and soil were 8.4-9.8 and 4.3-11.7 days, respectively. The final residues of chlorantraniliprole and thiamethoxam in maize straw, maize, and soil were measured after the pesticides had been sprayed two and three times with an interval of 7 days using 1 and 1.5 times the recommended rate (72 g a.i. ha(-1) and 108 g a.i. ha(-1), respectively). Representative maize straw, maize, and soil samples were collected after the last treatment at pre-harvest intervals of 7, 14, and 28 days. The chlorantraniliprole residue was below 0.01 mg kg(-1) in maize, between 0.01 and 0.31 mg kg(-1) in maize straw, and between 0.03 and 1.91 mg kg(-1) in soil. The thiamethoxam residue concentrations in maize, maize straw, and soil were <0.01, <0.01, and 0.01-0.03 mg kg(-1), respectively. The final pesticide residues on maize were lower than the maximum residue limit (MRL) of 0.02 mg kg(-1) after a 14-day pre-harvest interval. Therefore, a dosage of 72 g a.i. ha(-1) was recommended, as it can be considered safe to human beings and animals.

  9. Characterization of normal and waxy corn starch for bioethanol production.

    Science.gov (United States)

    Yangcheng, Hanyu; Jiang, Hongxin; Blanco, Michael; Jane, Jay-lin

    2013-01-16

    Objectives of this study were to compare ethanol production between normal and waxy corn using a cold fermentation process and to understand effects of starch structures and properties on ethanol production. Ethanol yields positively correlated (p starch contents of kernels of the normal and waxy corn. The average starch-ethanol conversion efficiency of waxy corn (93.0%) was substantially greater than that of normal corn (88.2%). Waxy corn starch consisted of very little amylose and mostly amylopectin that had a shorter average branch chain length than normal corn amylopectin. Regression analyses showed that average amylopectin branch chain lengths and percentage of long branch chains (DP > 37) of waxy corn starch negatively correlated with the starch hydrolysis rate and the ethanol yield. These results indicated that starch structures and properties of the normal and waxy corn had significant effects on the ethanol yield using a cold fermentation process.

  10. Resistance of corn genotypes to fall armyworm Spodoptera ...

    African Journals Online (AJOL)

    Tuoyo Aghomotsegin

    2016-08-31

    Aug 31, 2016 ... The objective of this study was to evaluate resistance mechanisms in 12 corn genotypes (transgenic hybrids: ..... FAW that fed on GM corn genotypes exhibited lower ... preference for other food types, particularly given the.

  11. Effect of corn cobs concentration on xylanase biosynthesis by ...

    African Journals Online (AJOL)

    Yomi

    2012-01-24

    Jan 24, 2012 ... INTRODUCTION. Corn cobs ... like corn cobs, sugar cane bagasse , rice husk, rice straw and oat straw ... Xylanase hydrolyzes the polymer xylan into the xylose monomers. The free ..... using central composite rotary design.

  12. High-Fructose Corn Syrup: What Are the Concerns?

    Science.gov (United States)

    Healthy Lifestyle Nutrition and healthy eating What is high-fructose corn syrup? What are the health concerns? Answers from Katherine Zeratsky, R.D., L.D. High-fructose corn syrup is a common sweetener in ...

  13. First occurrence of western corn root worm beetles in the federal states Hesse and Rhineland-Palatinate (Germany, 2011

    Directory of Open Access Journals (Sweden)

    Dicke, Dominik

    2014-02-01

    Full Text Available In 2011, western corn root worm beetles were detected in the federal states Hesse (Groß-Gerau and Rhineland-Palatinate (Bodenheim for the first time. Control measures based on commission decision 2003/766/EG (Byrne, 2003 were conducted after detection in PAL-traps. Focus and safety zones were established. In Hesse, both focus and safety zones were treated with the insecticide Biscaya, due to the high number of 50 beetles which were detected in the PAL-traps. Since in Rhineland-Palatinate, only one beetle had been captured, only the focus zone was treated with the insecticide. After insecticide treatment, new PAL-traps were arranged like a close grid over the infested areas in both federal states. In each maize field in the focus- and safety zone further traps were placed and checked weekly until September 30th by supporting staff. Until the end of the monitoring in 2011 (September 30th further beetles were detected in the south of the area (district of Groß-Gerau, Hesse, were the first infestation had been discovered. However, in Rhineland-Palatinate no further beetles were detected that year. By the end of the monitoring 354 beetles in Hesse and one beetle in Rhineland-Palatinate had been captured in total. Subsequently the demarked zones in Hesse were extended. Taking into account the local circumstances, the new focus zone was delimited to include all the areas where beetles had been detected as well as the surrounding maize fields. In the focus zones the cultivation of maize was forbidden for the consecutive two years and a crop rotation with at least 50 percent maize was established in the safety zones. In 2012 no further beetles were captured in the infested region.

  14. Assessment of Survival Competitiveness in Wasteland for Transgenic Bt Maize%抗虫转基因玉米荒地生存竞争力评价

    Institute of Scientific and Technical Information of China (English)

    陈小文; 李吉崇; 郭玉海; 董学会

    2012-01-01

    In order to better understand the survival capacity of the transgenic Bt maize and its receptor parent plant ( Zheng 58) ,we compared the survival competitiveness between weeds and maize separately and explored the possibility of maize evolved into weeds under field condition and artificial wasteland condition,which could provide theory basis for security assessment of transgenic Bt corn in ecological environment. The results showed that the transgenic Bt maize was more competitive than its receptor parent plant,but less competitive than weeds. The seeds of transgenic Bt maize also could live through the winter. Nonetheless,the trangenic Bt maize could not evolve into weeds in our test condition.%研究了转Cry1Ac基因玉米及受体非转基因玉米(郑58)在野外自然条件及模拟野外条件下的生存能力,比较玉米与杂草的竞争力及玉米自身成为杂草的可能性,为转抗虫基因玉米的环境生态安全评价提供理论基础.结果表明,生存竞争能力非转基因玉米<转基因玉米<杂草,转抗虫玉米虽然在与杂草的竞争中优于非转基因受体,其种子也能越冬,但在试验条件下不会演变成超级杂草.

  15. Gliricidia (Gliricidia sepium Green Manures as a Potential Source of N for Maize Production in the Tropics

    Directory of Open Access Journals (Sweden)

    Abdul R. Bah

    2001-01-01

    Full Text Available Use of cheap, N-rich, and environmentally benign legume green manures to correct N deficiency in infertile soils is a very attractive option in the humid tropics. Understanding the influence of management and climate on their effectiveness, and quantifying their contribution to crop productivity, is therefore crucial for technology adoption and adaptation. Mineral N buildup and the contribution to N uptake in maize were studied in an Ultisol amended with fresh Gliricidia leaves. Net mineral N accumulation was compared in mulched and incorporated treatments in a field incubation study. The 15N isotope dilution technique was used to quantify N supplied to maize by Gliricidia leaves in an alley cropping. Mineral N accumulation was slow, but was much greater after incorporation than after mulching. Also, N buildup was always higher in the topsoil (0 to 10 cm than in the subsoil (10 to 20 cm. More NO3-N was leached than NH4-N, and the effect was greater in the incorporated treatment. Surface-applied Gliricidia leaves significantly increased N uptake by maize, and supplied >30% of the total N in the stover and >20% of that in the corn grain, even in the presence of hedgerows. Thus Gliricidia leaf mulch has immense potential to improve productivity in tropical soils.

  16. [Accumulation of Mercury in Soil-maize System of Non-ferrous Metals Smelting Area and Its Related Risk Assessment].

    Science.gov (United States)

    Ji, Xiao-feng; Zheng, Na; Wang, Yang; Liu, Qiang; Zhang, Jing-jing

    2015-10-01

    Soil heavy metal pollution, especially the mercury pollution, has been widespread concern in non-ferrous metallurgical area. This study focused on the content, distribution and pollution status of Hg in maize soil of Huludao city. Meanwhile, Hg contents in the various organs of maize were analyzed. Hg concentration in soil ranged from 0.25 to 3.49 mg x kg(-1) with the average content of 1.78 mg x kg(-1), which was 48 times as high as the background value of Liaoning soil. Around 2-3m range of zinc plant, the pattern of spatial distribution showed that the content of Hg was gradually increased with the increase of the distance to Huludao zinc plant. The result of geoaccumulation index reflected that Hg pollution is up to moderate pollution level on the whole. 54. 6% of the total sample were belonged to the serious pollution level. The potential ecological risk index of Hakanson was applied to assess the ecological risk of Hg. The target hazard quotient method (THQ) was used to assess the health risk for human, the results revealed that there was no significant health risk by consumption corn. Mercury is very difficult to transport in soil-maize system, and there is no obvious health risks to adults. But the risk coefficient of children, which is up to 0.056. is much higher than adults.

  17. Best Management of Irrigation Fertilization to Sustain Environment and High yield of Maize in the Arid land in Egypt

    Science.gov (United States)

    Gameh Ali, M.

    2012-04-01

    Assiut is a county in the middle of Egypt,located 600 km south of the Mediterranean Sea. Water and fertilization management experimental trails were conducted to search for the best water consumption of Maize beside the best rate and type of nitrogen fertilization to reduce nitrate pollution and reduce fertilizer and save energy. Three irrigation regimes ( 25, 50, and 75% of soil moisture depletion of the available water, SMD) were used to irrigate Corn (Maize : Zea mays L. ) variety Tri hybrid cross. Three nitrogen fertilizer sources (Urea 46.5% N; Ammonium nitrate 33.5%N and slow release nitrogen 40%N) were applied at three rates of 90; 120 and 150 kg/ Feddan (4200m2 about one Acre). The results suggested that the best management is to use the slow release fertilizer at rate of 150 kg N/ Feddan (4200m2 ) with 50% SMD the highest Maize yield with good quality and reducing the environmental hazardous. Key words: Slow release fertilizer, Nitrogen leaching; Irrigation management. Environmental protection.

  18. Soil Fertility Status, Nutrient Uptake, and Maize (Zea mays L.) Yield Following Organic Matters and P Fertilizer Application on Andisol

    Science.gov (United States)

    Minardi, S.; Harieni, S.; Anasrullah, A.; Purwanto, H.

    2017-04-01

    Objective of this study were to elucidate effects of organic matters and P fertilizer application on soil fertility status, nutrient uptake and maize yield in the Andisol. This experiment consisted of two factors. The first factor comprised of four levels of organic matters input (without organic matter, manure, rice straw, and Gliricidia sepium leaves), with the application dosage 10 t.ha-1 and the second factor comprised of three levels of P fertilizer application (without P addition (control), 50 kg P2O5 ha-1, 100 kg P2O5 ha-1). Results of this study showed that organic matters and P fertilizer application improved soil fertility status, especially pH, soil organic C, cation exchange capacity (CEC), available P which resulted in an increase in P uptake that improve yield of maize. The highest yield of maize (corn cob) was obtained through application Gliricida sepium (8.40 t.ha-1), followed by manure (6.02 t.ha-1) and rice straw (5.87 t.ha-1). Application of 50 kg P2O5 Ha-1 yield was (5.76 t.ha-1) and application of 100 Kg P2O5 Ha-1 yield was (6.12 t.ha-1).

  19. Ethanol production from corn, corn stover and corncob from the Jilin Province of China

    Energy Technology Data Exchange (ETDEWEB)

    Varga, E.; Thomsen, A.B. [Risoe National Lab., Biosystem Dept., Roskilde (Denmark); Feng, L. [Jilin Light Industry Design and Reserch Inst., Changchun City (China)

    2005-06-01

    Among the available agricultural by-products, corn stover is far the most abundant lignocellulosic raw material for fuel ethanol production in China. More than 120 million tons of corn stover is produced annually, representing approximately 40 million tons of ethanol. In this study ethanol was produced from corn and alkaline wet oxidized (WO) corn stover and corncob followed by non-isothermal simultaneous saccharification and fermentation (SSF) using Saccharomyces cerevisiae. Eight different combinations of reaction temperature and time were applied for wet oxidation of corn stover and corncob using Na{sub 2}CO{sub 3} and NH{sub 3} as catalysts to find the best reaction conditions, resulting in both high glucose and ethanol yield. The best condition (200 deg. C, 8 min, 2g/L Na{sub 2}CO{sub 3}) increased the enzymatic conversion from cellulose to glucose of corn stover more then four times and resulted in 87% ethanol yield of theoretical, based on the cellulose available in the WO corn stover. This was achieved with a substrate concentration of 6% (w/w) dry material at 20 FPU/g DM enzyme loading after 120 h of SSF. The pretreatment with NH{sub 3} at the same conditions resulted slightly lower cellulose conversion to glucose, but also gave promising ethanol yield (75%), demonstrated, that the baker's yeast still could adapt to the WO material and ferment the glucose content to ethanol efficiently. (au)

  20. AFLATOXIN LEVELS IN LOCALLy GROWN MAIZE FROM MAKUENI ...

    African Journals Online (AJOL)

    2008-07-07

    Jul 7, 2008 ... Makueni District and to correlate aflatoxin levels to maize drying and storage practices. Also, ... Conclusion: High levels of aflatoxin in homegrown and purchased maize suggested that aflatoxin ..... Agriculture and Food Safety.

  1. Quality Protein Maize Response to Nitrogen Rate and Plant Density ...

    African Journals Online (AJOL)

    Quality Protein Maize Response to Nitrogen Rate and Plant Density in the Guinea Savanna Zone of Ghana. ... protein maize (Zea mays L.) hybrid to plant density and nitrogen (N) fertilizer. ... Optimal N rate was not affected by plant density.

  2. Effect of organic mulch materials on maize performance and weed ...

    African Journals Online (AJOL)

    Effect of organic mulch materials on maize performance and weed growth in the derived ... AFRICAN JOURNALS ONLINE (AJOL) · Journals · Advanced Search ... of organic mulch materials (Plant and Animal) on the performance of maize and ...

  3. Genetic architecture of domestication-related traits in maize

    Science.gov (United States)

    Strong directional selection occurred during the domestication of maize from its wild ancestor teosinte, reducing its genetic diversity, particularly at genes controlling domestication-related traits. Nevertheless, variability for some domestication-related traits is maintained in maize. The genet...

  4. Corn Heterotic Group and Model in Heilongjiang of China

    Institute of Scientific and Technical Information of China (English)

    JIN Yi; DONG Ling; YU Tianjiang; LI Yan; GUO Ran

    2009-01-01

    The concept and research achievements of the heterotic group and model in corn were introduced briefly. The results showed that the domestic corn germplasm could be divided into three main heterotic groups and two main heterotic models. The research on corn germplasm in Heilongjiang Province could be concluded as three main heterotic groups and three main heterotic models. Some new opinions about corn heterotic group and heterotic model in Heilongjiang Province were proposed such as Northeast group and NortheastxLancaster model.

  5. A method for sampling waste corn

    Science.gov (United States)

    Frederick, R.B.; Klaas, E.E.; Baldassarre, G.A.; Reinecke, K.J.

    1984-01-01

    Corn had become one of the most important wildlife food in the United States. It is eaten by a wide variety of animals, including white-tailed deer (Odocoileus virginianus ), raccoon (Procyon lotor ), ring-necked pheasant (Phasianus colchicus , wild turkey (Meleagris gallopavo ), and many species of aquatic birds. Damage to unharvested crops had been documented, but many birds and mammals eat waste grain after harvest and do not conflict with agriculture. A good method for measuring waste-corn availability can be essential to studies concerning food density and food and feeding habits of field-feeding wildlife. Previous methods were developed primarily for approximating losses due to harvest machinery. In this paper, a method is described for estimating the amount of waste corn potentially available to wildlife. Detection of temporal changes in food availability and differences caused by agricultural operations (e.g., recently harvested stubble fields vs. plowed fields) are discussed.

  6. Pretreatment of Corn Stalk by Steam Explosion

    Institute of Scientific and Technical Information of China (English)

    邵自强; 田永生; 谭惠民

    2003-01-01

    A steam explosion pretreatment, which is one of the best ways of pretreating plant stalk, is applied at various severities to corn stalk. It could effectively modify the super-molecular structure of corn stalk and defibrating corn stalk into individual components. The relationship between yield of reducing sugar and the operating conditions, including temperature, pressure of steam explosion pretreatment and acidity, is also established. Experimental results prove that the steam explosion substantially increases the yield of reducing sugar, and the optimal condition for steam explosion is as follows: the pressure is 2.0 MPa, the pressure-retaining time 300 s, the initial acid concentration 1% and the acid treatment time 24 h.

  7. 铁岭市玉米收获机械化发展思路探讨%Discussion on the Development of Mechanized Corn Harvesting in Tieling City

    Institute of Scientific and Technical Information of China (English)

    尤丹

    2014-01-01

    Tieling is known as"the Granary of North Liaoning", with maize as the first crop of the city, but the mechanized corn harvest-ing has remained the weakness in Tieling, so it is also the breakthrough in agricultural machinery popularization. This article reviews the development situation of mechanized corn harvesting in Tieling City, analyses factors affecting the quantity of corn harvester growth, and puts forward ideas on the development of mechanized corn harvesting.%铁岭素有“辽北粮仓”之称,玉米是铁岭第一大粮食作物,但玉米收获机械化一直是铁岭农业机械化的薄弱环节,也是农机推广工作的突破口。阐述铁岭地区玉米收获机械化的发展现状,分析影响玉米收获机数量增长的因素,介绍玉米收获机械化的发展思路。

  8. Research on Corn Borer Prevention by Using Beauveria Bassiana Sealing Method%白僵菌封垛防治玉米螟研究

    Institute of Scientific and Technical Information of China (English)

    陈佳广

    2014-01-01

    Corn borer is one of the main pests of maize crops. Using beauveria bassiana sealing method can decrease the quantity of corn borer larva. The article introduces the features and growing condition of corn borer, expounds the theory, methods, effects, considerations and advantages of using beauveria bassiana sealing method for corn borer prevention, provides a reference for the application and extension for the technique.%玉米螟是危害玉米作物的主要害虫之一,利用白僵菌可以有效减少玉米螟幼虫数量。介绍玉米螟的危害特点与发生条件,阐述白僵菌封垛防治玉米螟技术的原理、方法、效果、注意事项及优点,为该技术的推广应用提供参考。

  9. The microflora of fermented nixtamalized corn.

    Science.gov (United States)

    Sefa-Dedeh, Samuel; Cornelius, Beatrice; Amoa-Awua, Wisdom; Sakyi-Dawson, Esther; Afoakwa, Emmanuel Ohene

    2004-10-01

    Nixtamalization is a traditional process that improves the nutritional quality of corn. To provide a means of utilizing the nutritional benefits of nixtamalized corn and improve product acceptability, lactic acid fermentation was applied. The objective of the study was to study the microbial profile and establish the important lactobacilli of fermenting nixtamalized corn dough. Two batches of cleaned whole corn were subjected to the process of nixtamalization, using two concentrations of lime (0.5 or 1.0%), milled, made into a dough (50% moisture) and fermented spontaneously for 72 h. A control sample was prepared without alkaline treatment. pH and titratable acidity of the dough were measured. Aerobic mesophiles, lactic acid bacteria, yeasts and molds were enumerated on Plate Count Agar (PCA), deMan, Rogossa and Sharpe (MRS) Agar and Malt Extract Agar (MEA), respectively. The identity of lactobacilli present was established at the species level using API 50 CHL. The pH of all the fermenting systems decreased with fermentation time with concomitant increase in titratable acidity. Lactic acid bacteria in numbers of 1.6 x 10(9), 2.3 x 10(9) and 1.8 x 10(9) cfu/g, respectively yeasts and molds, and numbers of 8.0 x 10(7), 5.0 x 10(5) and 1.7 x 10(5) cfu/g, respectively were observed in the control and the two nixtamalized (0.5% and 1.0% lime) samples after 48 h of fermentation. Lactobacilli identified in the fermenting nixtamalized corn dough were Lactobacillus plantarum, Lactobacillus fermentum and Lactobacillus cellobiosus as well as Pediococcus spp. The study demonstrates that nixtamalized corn though alkaline in nature can be subjected to spontaneous fermentation to produce a sour product.

  10. Nutrient content in maize fertilized with tannery sludge vermicompost and irrigated with domestic wastewater

    Directory of Open Access Journals (Sweden)

    Guilherme Malafaia

    2016-11-01

    Full Text Available This study analyzed the macro and micronutrient content of maize leaves (Zea mays L. grown in soil containing tannery sludge vermicomposting and irrigated with wastewater. The arrangement of the treatments consisted of a factorial 2x6 (two types of irrigation and six kinds of fertilizer in a completely randomized design, with five repetitions, totaling sixty experimental units. The following experimental units, irrigated with supply water (A and household wastewater (R, were established: (T1 Control Soil, with no chemical fertilization and no vermicomposting; (T2 Soil + NPK; (T3 Soil + primary sludge vermicompost; (T4 Soil + P + primary sludge vermicompost; (T5 Soil + P + liming sludge vermicompost; and (T6 Soil + liming sludge vermicompost. For the leaf-tissue analysis, the opposite whole leaf below the first (upper ear was collected from each plant, excluding the midrib at the onset of the female inflorescence. The results showed that both wastewater and the tannery sludge vermicomposts can be a good source of nutrients for maize plants, since the macro and micronutrients in the leaves of plants were satisfactory and no signs or symptoms of toxicity were observed. While leaf analysis alone is insufficient to assess the nutritional status of plants, this study innovatively suggests the potential beneficial use of a combination of wastewater and tannery sludge vermicompost in the cultivation of corn, motivating new research.

  11. Genetics of Resistance and Pathogenicity in the Maize/Setosphaeria turcica Pathosystem and Implications for Breeding

    Directory of Open Access Journals (Sweden)

    Ana L. Galiano-Carneiro

    2017-08-01

    Full Text Available Northern corn leaf blight (NCLB, the most devastating leaf pathogen in maize (Zea mays L., is caused by the heterothallic ascomycete Setosphaeria turcica. The pathogen population shows an extremely high genetic diversity in tropical and subtropical regions. Varietal resistance is the most efficient technique to control NCLB. Host resistance can be qualitative based on race-specific Ht genes or quantitative controlled by many genes with small effects. Quantitative resistance is moderately to highly effective and should be more durable combatting all races of the pathogen. Quantitative resistance must, however, be analyzed in many environments (= location × year combinations to select stable resistances. In the tropical and subtropical environments, quantitative resistance is the preferred option to manage NCLB epidemics. Resistance level can be increased in practical breeding programs by several recurrent selection cycles based on disease severity rating and/or by genomic selection. This review aims to address two important aspects of the NCLB pathosystem: the genetics of the fungus S. turcica and the modes of inheritance of the host plant maize, including successful breeding strategies regarding NCLB resistance. Both drivers of this pathosystem, pathogen, and host, must be taken into account to result in more durable resistance.

  12. Optimum Leaf Removal Increases Nitrogen Accumulation in Kernels of Maize Grown at High Density

    Science.gov (United States)

    Liu, Tiening; Huang, Rundong; Cai, Tie; Han, Qingfang; Dong, Shuting

    2017-01-01

    Increasing plant density is one of the main approaches of achieving higher yields for modern maize crop. However, there exists leaf redundancy for high-density maize, and leaves of the upper canopy shade more competent leaves at the middle strata. In a two-year field experiments, Jinhai5, a semi-compact corn cultivar, was grown at a density of 105,000 plants ha−1 grown until 3 days after silking (3DAS), when plants were subjected to removal of the uppermost two leaves (S2), four leaves (S4) or six leaves (S6), with no leaf removal as control (S0). We evaluated the effects of leaf removal on N remobilization, photosynthetic capacity of the remaining leaves for N uptake, and N accumulation in kernels. Our present results concluded that, under high plant density, excising the uppermost two leaves promoted N remobilization from vegetative organs to kernels and enhanced photosynthetic capacity for N uptake, leading to an increased N accumulation in kernels (19.6% higher than control). However, four or six uppermost leaves removal reduced N remobilization from stem and photosynthesis for poor N uptake, resulting in 37.5 and 50.2% significantly reduced N accumulation in kernels, respectively. PMID:28084467

  13. Influence of soil on the efficacy of entomopathogenic nematodes in reducing Diabrotica virgifera virgifera in maize

    Science.gov (United States)

    Toepfer, Stefan; Kurtz, Benedikt

    2010-01-01

    The use of entomopathogenic nematodes is one potential non-chemical approach to control the larvae of the invasive western corn rootworm (Diabrotica virgifera virgifera LeConte, Coleoptera: Chrysomelidae) in Europe. This study investigated the efficacy of Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhabditidae), Heterorhabditis megidis Poinar, Jackson and Klein (Rh., Heterorhabditidae) and Steinernema feltiae Filipjev (Rh., Steinernematidae) in reducing D. v. virgifera as a function of soil characteristics. A field experiment was repeated four times in southern Hungary using artificially infested maize plants potted into three different soils. Sleeve gauze cages were used to assess the number of emerging adult D. v. virgifera from the treatments and untreated controls. Results indicate that nematodes have the potential to reduce D. v. virgifera larvae in most soils; however, their efficacy can be higher in maize fields with heavy clay or silty clay soils than in sandy soils, which is in contrast to the common assumption that nematodes perform better in sandy soils than in heavy soils. PMID:21124754

  14. Strategic Marketing Problems in the Uganda Maize Seed Industry

    OpenAIRE

    Larson,Donald W.; Mbowa, Swaibu

    2004-01-01

    Strategic marketing issues and challenges face maize seed marketing firms as farmers increasingly adopt hybrid varieties in a modernizing third world country such as Uganda. The maize seed industry of Uganda has changed dramatically from a government owned, controlled, and operated industry to a competitive market oriented industry with substantial private firm investment and participation. The new maize seed industry is young, dynamic, growing and very competitive. The small maize seed marke...

  15. Study Progress on Tissue Culture of Maize Mature Embryo

    Science.gov (United States)

    Wang, Hongzhen; Cheng, Jun; Cheng, Yanping; Zhou, Xioafu

    It has been paid more and more attention on maize tissue culture as it is a basic work in maize genetic transformation, especially huge breakthrough has been made in maize tissue culture utilizing mature embryos as explants in the recent years. This paper reviewed the study progress on maize tissue culture and plant regeneration utilizing mature embryos as explants from callus induction, subculture, plant regeneration and browning reduction and so on.

  16. 9 CFR 319.303 - Corned beef hash.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Corned beef hash. 319.303 Section 319... Products § 319.303 Corned beef hash. (a) “Corned Beef Hash” is the semi-solid food product in the form of a compact mass which is prepared with beef, potatoes, curing agents, seasonings, and any of the...

  17. 9 CFR 319.101 - Corned beef brisket.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Corned beef brisket. 319.101 Section... Smoked § 319.101 Corned beef brisket. In preparing “Corned Beef Brisket,” the application of curing solution to the beef brisket shall not result in an increase in the weight of the finished cured product...

  18. Visual responses of corn silk flies (Diptera: Ulidiidae)

    Science.gov (United States)

    Corn silk flies are major pests impacting fresh market sweet corn production in Florida and Georgia. Control depends solely on well-times applications of insecticides to protect corn ear development. Surveillance depends on visual inspection of ears with no effective trapping methods currently ava...

  19. Corn texture and particle size in broiler diets

    Directory of Open Access Journals (Sweden)

    MP Benedetti

    2011-12-01

    Full Text Available The objective of this study was to evaluate the effect of corn texture and the particle size on broiler performance, carcass yield, nutrient digestibility, and digestive organ morphometrics. In Experiment I, 720 male Cobb chicks were distributed in a completely randomized experimental design with a 2 x 3 factorial arrangement, consisting two corn textures (dented and hard and three corn particle sizes, was applied, with four replicates of 30 birds each. Corn particle size was classified according to geometric mean diameter (GMD as fine - 0.46 mm; medium - 0.73 mm, and coarse - 0.87 mm. In Experiment II, 120 broiler chicks were used to evaluate corn digestibility during the periods of 16 to 22 days and 35 to 41 days of age, using the method of total excreta collection. In Experiment I, corn particle size influenced body weight, average weight gain, feed intake and feed conversion ratio of 21-day-old birds. Corn texture and particle size did not affect the performance of 42-day-old broilers or carcass traits. In Experiment II, there was no influence of corn texture and particle size on digestive organ weights. Dented corn increased nitrogen excretion in the first trial, and hard corn improved dry matter digestibility in the second metabolic trial. Corn with fine particle size promotes better performance of broilers at 21 days of age. Hard corn results in higher dry matter digestibility and lower nitrogen excretion, and consequently higher production factor in 42-day-old broilers.

  20. Bacterial Diversity in Rhizospheres of Nontransgenic and Transgenic Corn

    OpenAIRE

    Fang, Min; Kremer, Robert J.; Peter P. Motavalli; Davis, Georgia

    2005-01-01

    Bacterial diversity in transgenic and nontransgenic corn rhizospheres was determined. In greenhouse and field studies, metabolic profiling and molecular analysis of 16S rRNAs differentiated bacterial communities among soil textures but not between corn varieties. We conclude that bacteria in corn rhizospheres are affected more by soil texture than by cultivation of transgenic varieties.

  1. Corn residue utilization by livestock in the USA

    Science.gov (United States)

    Corn (Zea mays L.) residue grazing or harvest provides a simple and economical practice to integrate crops and livestock. Limited information is available on how widespread corn residue utilization is practiced by US producers. In 2010, the USDA-ERS surveyed producers from 19 states on corn grain ...

  2. Production of ethanol and furfural from corn stover

    Science.gov (United States)

    Corn stover has potential for economical production of biofuels and value-added chemicals. The conversion of corn stover to sugars involves pretreatment and enzymatic hydrolysis. We have optimized hydrothermal, dilute H2SO4 and dilute H3PO4 pretreatments of corn stover for enzymatic saccharificati...

  3. Investigating the Effect of Soil Texture and Fertility on Evapotranspiration and Crop Coefficient of Maize Forage

    Directory of Open Access Journals (Sweden)

    M. Ghorbanian Kerdabadi

    2017-02-01

    Jey and Qahab district of Isfahan. Reference evapotranspiration and actual evapotranspiration of maize were measured by evaporation pan method and volumetric soil water balance method using micro lysimeters, respectively. In order to accommodate the growing field conditions, a ditch with a depth of 25 cm, length of 240 cm and width of 300 cm were dug and micro-lysimeters were placed it in three rows (three replications with a distance of 75 cm. After preparing the treatments, four seed Maize with variety of NS540 were planted at a depth of 3-5 cm on 5 August. To reduce the oasis effect on evapotranspiration, the same corn was planted in the vicinity of the project area with 500 square meters.. Results and Discussion: The results showed that using fertilizer caused increasing of crop evapotranspiration and crop coefficient of maize. Maximum of the ten-day average evapotranspiration of maize in the silty clay loam soil with two percent fertilizer was obtained 8.76 (mm/ day on the fifth decade of growth and this value was found 45.5 percent higher than the lowest mean evapotranspiration value of the ten-day. Comparison evapotranspiration of maize in different soil fertility treatments showed that the greatest impact on increasing of maize evapotranspiration in SLF2, SCLF2, SLF2 treatments were obtained that was equal %19.1, %14.3 and %10.6, respectively (table 4. Most of the effects of fertility the crop coefficient of maize at the middle stage of growth was influenced more than other stages by the different treatments of soil fertility. Adding one and two percent of the fertilizer to treatment SCLF0 increased maize crop coefficient about 3.5 and 9.7 percent at development stage, respectively, That measured %6 and 11% for LF1 and LF2 treatments, respectively, and about 1.6 and 5.6% for SLF2 SLF1 treatment, respectively (Table 6. Comparison of maize middle crop coefficient in SLF2 and SLF1 for different soil fertility treatments showed that effect of increasing soil

  4. The role of some agricultural practices and fertilizer type on both the incidence of stem borers infestation and corn yield in Egypt.

    Science.gov (United States)

    Mesbah, H A; Mourad, A K; el-Nimr, Hanyiat M; Massoud, M A; Abd el-Aziz, A A

    2002-01-01

    Maize, Zea mays, L. is one of the most important field crops in Egypt. It is used mainly for human, animal and poultry feeding. Corn plants are usually attacked by several injourious insect pests at different stages of development. Out of them, the pink stem borer, Sesamia cretica (Led.), the purple lined borer, Chilo agamemnon (Bles.), and the European corn borer Ostrinia nubilalis (Hb.); which cause great damage and yield losses. It is profitable to adopt an effective and sustainable strategy for controlling these insect-pests. In this concern, sowing dates, planting spaces, foliar fertilizers (macro and micro-nutrients), mineral and/or biofertilization, were investigated to evaluate their role as tools in the so-called Integrated Pest Management (IPM) program of corn pests. In general, the used planting spaces of 60 and 70 cm apart between furrows insignificantly affected the level of stem borers infestation. It was clearly observed that the sowing dates have a role in the incidence of stem borers infestation throughout the corn growing seasons of 1994 and 1995. Moreover, The biofertilized corn plants were more tolerant to the infestation by the stem borers than the minerally fertilized ones. Application of Polytrin significantly decreased the mean numbers of larvae. The tested nutrients preparations affected to less extent, the infestation levels. Concerning the interaction effect of applied nutrients preparations, used sowing dates and/or fertilizer type on the deduced means of larval numbers, it was revealed that: (i) the application of the nutrients preparations decreased to a great extent the effect of the studied sowing dates on the stem borers infestation; particularly in case of spraying ascorbic acid alone or in sequence with Polymex, coppersulphate & Potasin-F, (ii) the dressing of corn grains with the biofertilizers Phosphorin & Rhizobacterin before sowing, lowered to some extent the levels of infestation by Ch. agamemnon and O. nubilalis, in

  5. Effects of dry, wet, and rehydrated corn bran and corn processing method in beef finishing diets.

    Science.gov (United States)

    Macken, C N; Erickson, G E; Klopfenstein, T J; Milton, C T; Stock, R A

    2004-12-01

    Two finishing trials were conducted to determine the effects of adding different types of corn bran, a component of corn gluten feed, on cattle performance. In Trial 1, 60 English crossbred yearling steers (283 +/- 6.7 kg) were used in a completely randomized design with four dietary treatments. Treatments were diets with no corn bran, dry corn bran (86% DM), wet corn bran (37% DM), and rehydrated dry bran (37% DM). Bran was fed at 40% of dietary DM. All finishing diets had (DM basis) 9% corn steep liquor with distillers solubles, 7.5% alfalfa hay, 3% tallow, and 5% supplement. Gain efficiency and ADG were greater (P < 0.01) for cattle fed no corn bran compared with all treatments containing corn bran; however, no differences were detected across corn bran types. In Trial 2, 340 English crossbred yearling steers (354 +/- 0.6 kg) were used in a randomized block design with treatments assigned based on a 2 x 4 + 2 factorial arrangement (four pens per treatment). One factor was the corn processing method used (dry-rolled corn, DRC; or steam-flaked corn, SFC). The other factor was corn bran type: dry (90% DM), wet (40% DM), or dry bran rehydrated to 40 or 60% DM. Bran was fed at 30% of dietary DM, replacing either DRC or SFC. Two control diets (DRC and SFC) were fed with no added bran. All finishing diets contained (DM basis) 10% corn steep liquor with distiller's solubles, 3.5% alfalfa hay, 3.5% sorghum silage, and 5% supplement. Corn bran type did not affect DMI (P = 0.61), ADG (P = 0.53), or G:F (P = 0.10). Dry matter intake was greater (P < 0.01) by steers fed bran compared with those fed no bran, and was greater by steers fed DRC than by steers fed SFC (P < 0.01). Interactions occurred (P < 0.01) between grain source and bran inclusion for ADG and G:F. The ADG by steers fed the SFC diet without bran was greater (P < 0.01) than by steers fed SFC diets with bran, whereas the ADG by steers fed DRC diets with or without bran was similar. Daily gain was 15.2% greater

  6. Yield-trait performance landscapes: from theory to application in breeding maize for drought tolerance.

    Science.gov (United States)

    Messina, Carlos D; Podlich, Dean; Dong, Zhanshan; Samples, Mitch; Cooper, Mark

    2011-01-01

    The effectiveness of breeding strategies to increase drought resistance in crops could be increased further if some of the complexities in gene-to-phenotype (G → P) relations associated with epistasis, pleiotropy, and genotype-by-environment interactions could be captured in realistic G → P models, and represented in a quantitative manner useful for selection. This paper outlines a promising methodology. First, the concept of landscapes was extended from the study of fitness landscapes used in evolutionary genetics to the characterization of yield-trait-performance landscapes for agricultural environments and applications in plant breeding. Second, the E(NK) model of trait genetic architecture was extended to incorporate biophysical, physiological, and statistical components. Third, a graphical representation is proposed to visualize the yield-trait performance landscape concept for use in selection decisions. The methodology was demonstrated at a particular stage of a maize breeding programme with the objective of improving the drought tolerance of maize hybrids for the US Western Corn-Belt. The application of the framework to the genetic improvement of drought tolerance in maize supported selection of Doubled Haploid (DH) lines with improved levels of drought tolerance based on physiological genetic knowledge, prediction of test-cross yield within the target population of environments, and their predicted potential to sustain further genetic progress with additional cycles of selection. The existence of rugged yield-performance landscapes with multiple peaks and intervening valleys of lower performance, as shown in this study, supports the proposition that phenotyping strategies, and the directions emphasized in genomic selection can be improved by creating knowledge of the topology of yield-trait performance landscapes.

  7. Fertilizer effect Azolla - Anabaena in maize (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Aldás-Jarrín Juan Carlos

    2016-11-01

    Full Text Available The deterioration of natural resources in agricultural activities, has motivated the search for new alternatives to enrich the soil with macro and micro nutrients sustainably, for the benefit of future generations, just as you think about the conservation and maintenance of water, avoiding eutrophication problems by the accumulation of fertilizers (nitrates and nitrites leached and deposited in natural sources, for this purpose they have been measured several agro ecological options that provide proper nutrition and a delicate ecological balance; in this investigation at field level in maize cultivation and different states it applied dose of azolla as a natural source of nitrogen to enrich the soil in the canton Cevallos, Tungurahua province. Anabaena floors of the town, based on the state of azolla and the level established for such preparation, the states of azolla. Six mixtures were prepared substrates azolla studied were: Dry A1 and A2 in the fresh state; the doses were established in relation to the volume of azolla against soil volume used: (0.5: 1 (0.75: 1 and (1: 1. The data collected were plant height and percentage of nitrogen in dry matter at 15, 30, 60 and 90 days. All this in order to establish the amount of nitrogen contributed by azolla as biofertilizer in growing corn. The best results were presented at the A1D3 (azolla dry-Dose 1: 1 treatment given plant height 15.02 cm at 15 days, 35.88 cm 30 days, 53.22 cm 60 66.12 days and 90 days; to 0.54% nitrogen percentage at 15 days, 0.90% at 30 days, 1.68% at 60 days and 2.08% after 90 days. In conclusion, the use of Azolla as a bio-fertilizer rich in nitrogen is feasible, as has been demonstrated in this research that the corn plant benefits from the contribution of this material, improving sustainable agricultural practices. It is recommended to expand this research in the same crop or other commercially important crops until the end of its production cycle, to report the results of

  8. Greenhouse-selected resistance to Cry3Bb1-producing corn in three western corn rootworm populations.

    Directory of Open Access Journals (Sweden)

    Lisa N Meihls

    Full Text Available Transgenic corn producing the Bacillus thuringiensis (Bt toxin Cry3Bb1 has been useful for controlling western corn rootworm, Diabrotica virgifera virgifera LeConte, one of the most economically important crop pests in the United States. However, rapid evolution of resistance by this beetle to Bt corn producing Cry3Bb1 has been reported previously from the laboratory, greenhouse, and field. Here we selected in the greenhouse for resistance to Cry3Bb1 corn in three colonies of WCR derived from Kansas, Minnesota, and Wisconsin, respectively. Three generations of rearing on Cry3Bb1 corn significantly increased larval survival on Cry3Bb1 corn, resulting in similar survival in the greenhouse for selected colonies on Cry3Bb1 corn and isoline corn that does not produce Bt toxin. After four to seven generations of rearing on Cry3Bb1 corn, survival in the field on Cry3Bb1 corn relative to isoline corn more than doubled for selected colonies (72% compared with control colonies (33%. For both selected and control colonies, survival in the field was significantly lower on Cry3Bb1 corn than on isoline corn. On isoline corn, most fitness components were similar for selected colonies and control colonies. However, fecundity was significantly lower for selected colonies than control colonies, indicating a fitness cost associated with resistance. The rapid evolution of resistance by western corn rootworm to Bt corn reported here and previously underlines the importance of effective resistance management for this pest.

  9. Mixed cropping of groundnuts and maize in East Java

    NARCIS (Netherlands)

    Hoof, van W.C.H.

    1987-01-01

    Mixed cropping of groundnuts and maize in East Java was studied by means of a survey of farming practice and by field experiments. The influence of different sowing times and plant density of maize on the development and yield of groundnuts and maize were the main topics in this thesis. Plant arrang

  10. Quantitative disease resistance: dissection and adoption in maize

    Science.gov (United States)

    Maize is the world’s most widely cultivated crop, providing food, feed, and biofuel. Maize production is constantly threatened by the presence of devastating pathogens worldwide. Characterization of the genetic components underlying disease resistance is a major research area in maize which is highl...

  11. Mixed cropping of groundnuts and maize in East Java.

    NARCIS (Netherlands)

    Hoof, van W.C.H.

    1987-01-01

    Mixed cropping of groundnuts and maize in East Java was studied by means of a survey of farming practice and by field experiments. The influence of different sowing times and plant density of maize on the development and yield of groundnuts and maize were the main topics in this thesis. Plant arrang

  12. Fertilizer use efficiency by maize (Zea mays) and egusi- melon ...

    African Journals Online (AJOL)

    DBOY

    fertilizers by maize and egusi-melon in various ratios of mixtures in an ultisol in Nigeria. The experiment ... Fertilizer use efficiency (FUE) was generally higher in .... two equal splits at 3 and 8 weeks after planting (WAP) coinciding .... Effects of cropping ratios of maize and Egusi-melon on yield and yield components of maize.

  13. Oven-drying reduces ruminal starch degradation in maize kernels

    NARCIS (Netherlands)

    Ali, M.; Cone, J.W.; Hendriks, W.H.; Struik, P.C.

    2014-01-01

    The degradation of starch largely determines the feeding value of maize (Zea mays L.) for dairy cows. Normally, maize kernels are dried and ground before chemical analysis and determining degradation characteristics, whereas cows eat and digest fresh material. Drying the moist maize kernels (consist

  14. Oven-drying reduces ruminal starch degradation in maize kernels

    NARCIS (Netherlands)

    Ali, M.; Cone, J.W.; Hendriks, W.H.; Struik, P.C.

    2014-01-01

    The degradation of starch largely determines the feeding value of maize (Zea mays L.) for dairy cows. Normally, maize kernels are dried and ground before chemical analysis and determining degradation characteristics, whereas cows eat and digest fresh material. Drying the moist maize kernels

  15. Biochar mitigation of allelopathy induced yield loss in continuous maize

    Science.gov (United States)

    Continuous maize yields are limited by the release of phytotoxic compounds as the previous year’s maize residue decomposes. We tested the hypothesis that soil biochar applications could help mitigate maize autotoxicity and the associated yield depression. Eighteen small field plots (23.7 m2) were es...

  16. Updates to the Corn Ethanol Pathway and Development of an Integrated Corn and Corn Stover Ethanol Pathway in the GREET™ Model

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Zhichao [Argonne National Lab. (ANL), Argonne, IL (United States). Energy Systems Division; Dunn, Jennifer B. [Argonne National Lab. (ANL), Argonne, IL (United States). Energy Systems Division; Wang, Michael Q. [Argonne National Lab. (ANL), Argonne, IL (United States). Energy Systems Division

    2014-09-01

    Corn ethanol, a first-generation biofuel, is the predominant biofuel in the United States. In 2013, the total U.S. ethanol fuel production was 13.3 billion gallons, over 95% of which was produced from corn (RFA, 2014). The 2013 total renewable fuel mandate was 16.6 billion gallons according to the Energy Independence and Security Act (EISA) (U.S. Congress, 2007). Furthermore, until 2020, corn ethanol will make up a large portion of the renewable fuel volume mandated by Renewable Fuels Standard (RFS2). For the GREET1_2014 release, the corn ethanol pathway was subject to updates reflecting changes in corn agriculture and at corn ethanol plants. In the latter case, we especially focused on the incorporation of corn oil as a corn ethanol plant co-product. Section 2 covers these updates. In addition, GREET now includes options to integrate corn grain and corn stover ethanol production on the field and at the biorefinery. These changes are the focus of Section 3.

  17. The Amount and Timing of Foliar Urea Application Effect on Maize and Forage Sorghum Proteins

    Directory of Open Access Journals (Sweden)

    H. Kheirabadi

    2012-11-01

    Full Text Available This study was carried out for investigating the effect of amount and timing of foliar urea in increasing silage yield and protein content of fodder, in a maize field in Neyshabour, Khorasan Razavi Province during 2011 summer crop season. A split plot design with factorial arrangement and three replications were used. Treatments were timing of foliar urea application (a week before tasselling, mid anthesis and early milk stage, urea levels (zero (check, five and 7.5 g.lit-1 and two hybrids of corn (Sc 704 and forage sorghum (speed feed. The results indicated that the concentration of nitrogen had significant effect to increase forage protein and silage yield (correcting by 280 g.kg-1 of dry matter. The highest forage protein concentrations for both crops and silage yield were achieved with 7.5 g.lit-1 of urea application, but the silage yield of sorghum was more than that of corn. The results showed that urea spraying can effectively increase the fodder nitrogen and silage protein, respectively, with the least possible cost, and in this respect it has an important role in providing quality and environmental safe forage for producer and consumer, meanwhile the adverse effects of excessive use of nitrogen fertilizer are reduced.   

  18. Response model of vegetation parameters and yield in maize under the influence of Lithovit fertilizer

    Science.gov (United States)

    Gigel, Prisecaru; Florin, Sala

    2017-07-01

    Control of plant nutrition and photosynthetic processes through fertilization is a necessary and current practice, to corn crop. This study aimed to assess the influence of Lithovit fertilizer, a product with a balanced content of nutrients, on chlorophyll content as an indicator of physiological status of plants and production of maize, hybrid Andreea. In the range of concentrations tested (0.3%, 0.5%, 0.75% and 1%), along with a control variant, product Lithovit generated the best results at concentrations of 0.5% and 0.7%, safely statistics (p < 0.01). Based on the values of chlorophyll content in the stages of growth, BBCH 30 (R2 = 0.998, p <0.001) and BBCH 63 (R2 = 0.992, p <0.001), it was possible, by regression analysis, to predict the production of corn, safely statistics. The behavior of response curves was described by polynomial functions of order 3, which capture the inflection points as a result of how to variation of chlorophyll content, amid treatments applied.

  19. Baby corn, green ear, and grain yield of corn cultivars Rendimentos de minimilho, de espigas verdes e de grãos de cultivares de milho

    Directory of Open Access Journals (Sweden)

    Itala Paula de C. Almeida

    2005-12-01

    Full Text Available Most maize cultivars have been developed for grain production. Because superior cultivars may differ in their exploiting purposes, interest has been demonstrated for the evaluation of corn cultivars with regard to their baby corn, green ear, and grain yields production ability. In the present work ten corn cultivars (AG 405, AG 1051, AG 2060, AG 6690, AG 7575, AG 8080, DKB 333 B, DKB 435, DKB 350 and DKB 747 were evaluated in the yield of baby corn, green ears and dry grains. Two experiments were carried out in the same season, in neighboring areas and managed in a similar way, in Mossoró, Rio Grande do Norte State, Brazil, in a randomized blocks design with five replicates. Baby corn yield (178,571 plants ha-1 was evaluated in one of the experiments. The other experiment (50,000 plants ha-1 was set to evaluate green ear and dry grain yield. Cultivars DKB 350 and AG 8080 were the most productive in number and weight of marketable unhusked, and husked baby corn ears. Cultivars DKB 435 and AG 8080 were the most productive in number and weight of marketable, unhusked, and husked ears. There were no differences between cultivars for grain yield.Desde que a maioria das cultivares de milho foi desenvolvida para produção de grãos, existe interesse em se avaliá-las quanto à produção de minimilho e espigas verder pois as cultivares superiores podem diferir, dependendo da finalidade de exploração da cultura. O objetivo do trabalho foi avaliar as produções de minimilho, espigas verdes e de grãos de dez cultivares (AG 405, AG 1051, AG 2060, AG 6690, AG 7575, AG 8080, DKB 333 B, DKB 435, DKB 350 e DKB 747 de milho. Dois experimentos, conduzidos na mesma época, em áreas vizinhas e manejados de forma semelhante, foram realizados em Mossoró-RN, no delineamento de blocos ao acaso com cinco repetições. Em um deles (178.571 plantas ha-1 avaliou-se a produção de minimilho. No outro (50.000 plantas ha-1, avaliaram-se as produções de espigas

  20. 防治玉米顶腐病和黑粉病药剂筛选%Fungicides for controlling the corn top-rot and corn smut diseases

    Institute of Scientific and Technical Information of China (English)

    邢会琴; 马建仓; 许永锋; 李万苍

    2011-01-01

    采用10种杀菌剂对玉米顶腐病菌室内测定结果表明,80%多·福·福锌可湿性粉剂、70%多·福可湿性粉剂、97%多菌灵可湿性粉剂和98%烯唑醇可湿性粉剂抑菌效果最好,而且持效期较长.以97%多菌灵可湿性粉剂和98%烯唑醇可湿性粉剂按3∶1或4∶1的比例进行复配,抑菌效果达到了其各自单用的抑菌效果.经安全性测定,用98%烯唑醇可湿性粉剂进行种子包衣在低温条件下对玉米出苗和苗期生长有一定影响,97%多菌灵可湿性粉剂则无影响.田间防治试验表明,97%多菌灵可湿性粉剂或97%多菌灵可湿性粉剂与98%烯唑醇可湿性粉剂复配对玉米顶腐病和瘤黑粉病均有一定防效.在玉米4~8叶和8~12叶期时,以97%多菌灵可湿性粉剂20 g+有机硅5mL/666.7m2和97%多菌灵可湿性粉剂25 g+98%烯唑醇可湿性粉剂5 g+有机硅7mL/666.7 m2用量分别喷洒,对顶腐病的防治效果达90%左右,并可兼治瘤黑粉病,防治效果达70%左右.%Ten fungicides were selected to control Fusariwn subglutinans in the laboratory. The results showed that 80% carbendazim ? Thiram ? Ziram WP, 70% carbendazim ? Thiram WP, 97% carbendazim WP and 98% diniconazole WP could remarkably inhibit pathogens, and the inhibition duration was long. The inhibition effect of 97% carbendazim ? 98% diniconazole WP, with the ratio of 3:1 or 4 :1, had the same effect as 97% carbendazim WP or 98% diniconazole WP. Safety testing showed that 98% diniconazole WP seriously affected the germination of maize seeds and growth of seedlings, while 97% carbendazim WP had no effects on the germination of maize seeds and growth of seedlings. Control efficacy tests in fields indicated that the control effect of 97% carbendazim WP or 97% carbendazim ? 98% diniconazole WP could control corn top rot and corn smut diseases. By spraying 20 g 97% carbendazim WP and 5 mL silicone per 666. 7 m2 or 25 g 97% carbendazim WP and 5 g 98

  1. Cannibalism of Helicoverpa zea (Lepidoptera: Noctuidae) from Bacillus thuringiensis (Bt) transgenic corn versus non-Bt corn.

    Science.gov (United States)

    Chilcutt, Charles F

    2006-06-01

    Because of the importance of cannibalism in population regulation of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in corn, Zea mays L., it is useful to understand the interactions between Bacillus thuringiensis (Bt) transgenic corn and cannibalism. To determine the effects of Bt corn on cannibalism in H. zea, pairs of the same or different instars were taken from Bt or non-Bt corn and placed on artificial diet in proximity. Cannibalism occurred in 91% of pairs and was approximately 7% greater for pairs of larvae reared from Bt transgenic corn (95%) than from non-Bt corn (88%). Also, first instar by first instar pairs had a lower rate of cannibalism than other pairs. Time until cannibalism was not different for larvae from Bt corn versus non-Bt corn. Pupation rate of cannibals and surviving victims was not different for pairs from Bt corn versus non-Bt corn. Finally, cannibalism increased pupation rate of cannibals from both Bt and non-Bt corn by approximately 23 and 12%, respectively, although the increases were not significant. Thus, negative effects of Bt on larvae were compensated by increased cannibalism in comparison with larvae reared on non-Bt corn, which increased larval survival to levels comparable with larvae reared on non-Bt plants.

  2. [Effects of phytase transgenic corn planting on soil nematode community].

    Science.gov (United States)

    Zhao, Zong-Chao; Su, Ying; Mou, Wen-Ya; Liu, Man-Qiang; Chen, Xiao-Yun; Chen, Fa-Jun

    2014-04-01

    A healthy soil ecosystem is essential for nutrient cycling and energy conversion, and the impact of exogenous genes from genetically modified crops had aroused wide concerns. Phytase transgenic corn (i. e., the inbred line BVLA430101) was issued a bio-safety certificate on 27 September 2009 in China, which could improve the efficiency of feed utilization, reduce environmental pollution caused by animal manure. In this study, the abundance of trophic groups, community structure and ecological indices of soil nematodes were studied over the growing cycle of phytase transgenic corn (ab. transgenic corn) and control conventional parental corn (ab. control corn) in the field. Totally 29 and 26 nematode genera were isolated from transgenic corn and control corn fields, respectively. The abundances of bacterivores and omnivores-predators, the total number of soil nematodes, and the Shannon index (H) were significantly greater under transgenic corn than under control corn, while the opposite trend was found for the relative abundance of herbivores and the maturity index (Sigma MI) of soil nematodes. Repeated-measures analysis of variance (ANOVA) did not detect any significant effects of transgenic corn on the composition and abundance of nematode trophic groups and ecological indices of soil nematodes. Furthermore, the Student-T test showed that the abundances of bacterivores and omnivores-predators and the total number of soil nematodes during the milk-ripe stage were significant higher in the transgenic corn field than in the control corn field. The effects of transgenic corn planting on soil nematodes might be related to the increase in the nitrogen content of field soil under transgenic corn compared to control corn.

  3. Screening and Identification of Maize Hybrids Resistance to Fusarium Ear Rot%玉米杂交种抗镰孢菌穗腐病鉴定筛选

    Institute of Scientific and Technical Information of China (English)

    赵杨; 陈靖; 徐秀德; 董怀玉; 王丽娟; 姜钰; 胡兰

    2011-01-01

    采用双牙签法将玉米穗腐病病原串珠镰刀菌和禾谷镰孢菌混合物接种到50份玉米杂交种上,对其进行抗穗腐病鉴定,筛选出高抗(HR)玉米杂交种2份,抗病(R)玉米杂交种31份.%In order to identify the resistance to Fusarium ear rot, fifty corn hybrids were inoculated with the mixture of F. Vertcillioides and F. Graminearum by double toothpick method. The result showed that two corn hybrids were highly resistant and 31 maize hybrids were resistant.

  4. Climatic and non-climatic drivers of spatiotemporal maize-area dynamics across the northern limit for maize production

    DEFF Research Database (Denmark)

    Odgaard, Mette Vestergaard; Bøcher, Peder Klith; Dalgaard, Tommy

    2011-01-01

    density [in Denmark, maize is mainly grown for ensilage to feed livestock], and sandy soil [facilitates maize growing in cold areas due to higher soil temperature than loamy soils]). Our results indicate that there has been a geographical expansion of maize in Denmark from 1999 to 2008, with a strong link...

  5. Corn processing method in finishing diets containing wet corn gluten feed.

    Science.gov (United States)

    Scott, T L; Milton, C T; Erickson, G E; Klopfenstein, T J; Stock, R A

    2003-12-01

    Two trials were conducted to determine the effect of corn processing method on performance and carcass traits in steers fed finishing diets containing wet corn gluten feed (WCGF). In Trial 1, 480 steer calves (303 kg initial BW) were fed eight finishing diets: 1) dry-rolled corn (DRC) without; and 2) with 32% (DM basis) WCGF; 3) steam-flaked corn (SFC) without; and 4) with WCGF; 5) a combination of DRC and SFC without WCGF; 6) finely-ground corn (FGC) with WCGF; 7) high-moisture corn (HMC) with WCGF; and 8) whole corn (WC) with WCGF. Feeding WC + WCGF increased (P < 0.10) DMI and decreased gain:feed compared with all other treatments. Feeding DRC + WCGF increased (P < 0.10) DMI and decreased (P < 0.10) gain:feed compared with treatments other than WC + WCGF. Steers on treatments that included WCGF gained similarly, regardless of corn processing method, and at a rate 6% faster (P < 0.10) than steers fed diets that did not include WCGF. Gain:feed did not differ among steers fed SFC, SFC + WCGF, SFC + DRC, and HMC + WCGF. Steers fed SFC or SFC + WCGF were more efficient (P < 0.10) than steers fed DRC or FGC + WCGF. In Trial 2, 288 steer calves (382 kg initial BW) were fed six finishing diets: 1) DRC without; and 2) with 22% (DM basis) WCGF; 3) SFC without; and 4) with WCGF; 5) finely rolled corn (FRC) with WCGF; and 6) HMC corn with WCGF. Steers fed DRC + WCGF or FRC + WCGF consumed more DM (P < 0.10) than steers fed DRC, SFC, or SFC + WCGF. Feed intake did not differ between steers fed SFC + WCGF and HMC + WCGF. All treatment groups receiving WCGF consumed more DM (P < 0.10) feed than steers fed DRC or SFC without WCGF. Steers fed SFC + WCGF gained 8% faster (P < 0.10), and steers fed DRC 9.5% slower (P < 0.10) than steers receiving all other treatments. Daily gains did not differ among other treatment groups. Steers fed SFC or SFC + WCGF gained 10% more (P < 0.10) efficiently than all other treatment groups. Feed efficiency did not differ among steers fed DRC, DRC

  6. Household dietary exposure to aflatoxins from maize and maize products in Kenya.

    Science.gov (United States)

    Kilonzo, Robert M; Imungi, Jasper K; Muiru, William M; Lamuka, Peter O; Njage, Patrick M Kamau

    2014-01-01

    Aflatoxicosis has repeatedly affected Kenyans, particularly in the eastern region, due to consumption of contaminated maize. However, save for the cases of acute toxicity, the levels of sub-lethal exposure have not been adequately assessed. It is believed that this type of exposure does exist even during the seasons when acute toxicity does not occur. This study, therefore, was designed to assess the exposure of households to aflatoxins through consumption of maize and maize products. Twenty samples each of maize kernels, muthokoi and maize meal were randomly sampled from households in Kibwezi District of Makueni County in Eastern Kenya and analysed for aflatoxin contamination. The samples were quantitatively analysed for aflatoxin contamination using HPLC. The uncertainty and variability in dietary exposure was quantitatively modelled in Ms Excel using Monte Carlo simulation in @Risk software. Aflatoxins were found in 45% of maize kernels at between 18 and 480 μg kg⁻¹, 20% of muthokoi at between 12 and 123 μg kg⁻¹, and 35% of maize meal at between 6 and 30 μg kg⁻¹. The mean dietary exposure to aflatoxin in maize kernels was 292 ± 1567 ng kg⁻¹ body weight day⁻¹, while the mean dietary exposure to aflatoxin in maize meal and muthokoi were 59 ± 62 and 27 ± 154 ng kg⁻¹ body weight day⁻¹ respectively. The results showed that the amount and frequency of consumption of the three foods is the more important contributing factor than the mean aflatoxin concentration levels, to the risk of dietary exposure to aflatoxins.

  7. An Economic Analysis of Corn-based Ethanol Production

    OpenAIRE

    Koo, Won W.; Taylor, Richard D.

    2008-01-01

    A global multi-commodity simulation model was developed to estimate the impact of changes in ethanol production on the U.S. corn industry. Increased ethanol production under the Energy Acts of 2005 and 2007 resulted in a significant increase in the price of corn. However, for corn-based ethanol production, the break-even price of corn is approximately $4.52 per bushel with a federal subsidy of $0.51 per gallon of pure ethanol and $2.50 gasoline. With a corn price of $4.52, the economically de...

  8. The Response of Corn Acreage to Ethanol Plant Siting

    OpenAIRE

    Fatal, Yehushua S.; Thurman, Walter N.

    2014-01-01

    U.S. ethanol production capacity increased more than threefold between 2002 and 2008. We study the effect of this growth on corn acreage. Connecting annual changes in county-level corn acreage to changes in ethanol plant capacities, we find a positive effect on planted corn. The building of a typical plant is estimated to increase corn in the county by over 500 acres and to increase acreage in surrounding counties up to almost 300 miles away. All ethanol plants are estimated to increase corn ...

  9. Corn Stover Impacts on Near-Surface Soil Properties of No-Till Corn In Ohio

    Energy Technology Data Exchange (ETDEWEB)

    Blanco-Canqui, H; Lal, Rattan; Post, W M.; Izaurralde, R Cesar C.; Owens, L B.

    2006-01-06

    Corn stover is a primary biofuel feedstock and its expanded use could help reduce reliance on fossil fuels and net CO2 emissions. Excessive stover removal may, however, negatively impact near-surface soil properties within a short period after removal. We assessed changes in soil crust strength, bulk density, and water content over a 1-yr period following a systematic removal or addition of stover from three no-till soils under corn in Ohio.

  10. Effects of bacillus thuringiensis transgenic corn on corn earworm and fall armyworm (Lepidoptera: Noctuidae) densities.

    Science.gov (United States)

    Chilcutt, Charles F; Odvody, Gary N; Correa, J Carlos; Remmers, Jeff

    2007-04-01

    We examined 17 pairs of near-isogenic hybrids of Bacillus thuringiensis (Bt) (176, Mon810, and Bt11) and non-Bt corn, Zea mays L., to examine the effects of Bt on larval densities of Helicoverpa zea (Boddie) and Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) during 2 yr. During ear formation, instar densities of H. zea and S. frugiperda were recorded for each hybrid. We found that H. zea first, second, and fifth instar densities were each affected by Mon810 and Bt11 Bt corn but not by 176 corn. Surprisingly, first and second instars were found in higher numbers on ears of Mon810 and Bt11 corn than on non-Bt corn. Densities of third and fourth instars were equal on Bt and non-Bt hybrids, whereas densities of fifth instars were lower on Bt plants. S. frugiperda larval densities were only affected during 1 yr when second, and fourth to sixth instars were lower on ears of Mon810 and Bt11 hybrids compared with their non-Bt counterparts. Two likely explanations for early instar H. zea densities being higher on Bt corn than non-Bt corn are that (1) Bt toxins delay development, creating a greater abundance of early instars that eventually die, and (2) reduced survival of H. zea to later instars on Bt corn decreased the normal asymmetric cannibalism or H. zea-S. frugiperda intraguild predation of late instars on early instars. Either explanation could explain why differences between Bt and non-Bt plants were greater for H. zea than S. frugiperda, because H. zea is more strongly affected by Bt toxins and more cannibalistic.

  11. Controle químico da cigarrinha-do-milho e incidência dos enfezamentos causados por molicutes Chemical control of corn leafhopper and incidence of corn stunting diseases caused by mollicutes

    Directory of Open Access Journals (Sweden)

    Charles Martins de Oliveira

    2007-03-01

    Full Text Available O objetivo deste trabalho foi verificar a eficiência do tratamento inseticida de sementes de milho no controle de Dalbulus maidis e na redução da incidência de enfezamentos em viveiro telado e em campo. Foram realizados dois experimentos; no experimento 1, em viveiro telado, sementes de milho foram tratadas com imidacloprid e thiamethoxan e, nessas plantas, cigarrinhas sadias, cigarrinhas infectantes com fitoplasma ou com espiroplasma foram confinadas. Avaliaram-se eficiência de controle, incidência de plantas com enfezamentos, altura das plantas e produção de grãos. No experimento 2, em campo, imidacloprid e thiamethoxan foram utilizados em tratamento de sementes e pulverizações aos 10 e 20 dias após a semeadura. A incidência de enfezamentos e a produção de grãos foram avaliadas. Em viveiro telado, os produtos imidacloprid e thiamethoxan proporcionaram controle de adultos de D. maidis acima de 50%, até o trigésimo dia, e reduziram a incidência de doenças e danos no crescimento e produção das plantas infectadas expostas às cigarrinhas infectantes aos dois dias após a emergência. Em campo, não foi constatada redução na incidência de enfezamentos ou ganho em produção, possivelmente devido ao fluxo migratório de cigarrinhas infectantes.The objective of this work was to verify the efficiency of maize seed treatment on Dalbulus maidis control, and its effect on corn stunting diseases incidence, at screenhouse and at field. Two experiments were carried out. In the experiment 1 ( screenhouse, maize seeds were treated with imidacloprid or with thiamethoxan. Healthy leafhoppers, phytoplasma infective leafhoppers, and spiroplasma infective leafhoppers were confined in plants. Efficiency of control, incidence of plants with corn stunting diseases, plant height, and grain production were evaluated. In the experiment 2 (field imidacloprid and thiamethoxan were used for seed treatment and sprayed at 10 and 20 days after sowing

  12. Liquefaction, saccharification, and fermentation of ammoniated corn to ethanol.

    Science.gov (United States)

    Taylor, Frank; Kim, Tae Hyun; Abbas, Charles A; Hicks, Kevin B

    2008-01-01

    Treatment of whole corn kernels with anhydrous ammonia gas has been proposed as a way to facilitate the separation of nonfermentable coproducts before fermentation of the starch to ethanol, but the fermentability of ammoniated corn has not been thoroughly investigated. Also, it is intended that the added ammonia nitrogen in ammonia treated corn (approximately 1 g per kg corn) may satisfy the yeast nutritional requirement for free amino nitrogen (FAN). In this study, procedures for ammoniation, liquefaction, saccharification, and fermentation at two scales (12-L and 50-mL) were used to determine the fermentation rate, final ethanol concentration, and ethanol yield from starch in ammoniated or nonammoniated corn. The maximum achievable ethanol concentration at 50 h fermentation time was lower with ammoniated corn than with nonammoniated corn. The extra nitrogen in ammoniated corn satisfied some of the yeast requirements for FAN, thereby reducing the requirement for corn steep liquor. Based upon these results, ammoniation of corn does not appear to have a positive impact on the fermentability of corn to ethanol. Ammoniation may still be cost effective, if the advantages in terms of improved separations outweigh the disadvantages in terms of decreased fermentability.

  13. Aminoácidos Digestíveis Verdadeiros do Milheto, do Milho e Subprodutos do Milho, Determinados com Galos Adultos Cecectomizados True Digestible Amino Acids of the Millet, Corn and Corn Byproducts, Determined with Cecectomized Adult Cockerels

    Directory of Open Access Journals (Sweden)

    Paulo Borges Rodrigues

    2001-12-01

    Full Text Available Os coeficientes de digestibilidade e o conteúdo de aminoácidos digestíveis verdadeiros do milho e seus subprodutos e do milheto foram determinados utilizando-se galos cecectomizados, em que 11 alimentos testados foram fornecidos a seis galos (15 g pela manhã e 15 g à tarde, após jejum de 24 horas e seis galos foram mantidos em jejum para determinação das perdas endógenas. Após obtidos os coeficientes de digestibilidade e calculados os aminoácidos digestíveis, ajustaram-se equações para predizer a composição do milho e seus subprodutos em lisina (LIS, metionina (MET, metionina + cistina (M+C, treonina (TRE e arginina (ARG, totais e digestíveis, em função da composição em proteína bruta (PB, fibra bruta (FB, extrato etéreo (EE e matéria mineral (MM. Os coeficientes médios de digestibilidade dos aminoácidos (essenciais e não-essenciais das amostras de milho 1, 2, Quality Protein Maize (QPM e pré-cozido, farelos de glúten 60 e 21, gérmen de milho, gérmens de milho fino e desengordurado e dos milhetos 1 e 2 foram 92,02 e 90,69; 88,62 e 87,33; 88,40 e 85,75; 90,20 e 88,99; 95,04 e 94,84; 82,11 e 79,10; 91,84 e 91,30; 92,56 e 91,59; 87,92 e 86,18; 91,04 e 88,70; 93,55 e 93,79%, respectivamente. O milheto apresentou aminoácidos de alta digestibilidade, semelhante ao milho. As equações LIS = 0,26488 + 0,01198PB; MET = -0,01315 + 0,02031PB; M+C = 0,07769 + 0,03448PB; TRE = 0,05576 + 0,03410PB e ARG = 0,30805 + 0,02370PB e LIS = 0,23518 + 0,01067PB; MET = -0,02198 + 0,0195PB; M+C = 0,04767 + 0,03143PB; TRE = -0,01738 + 0,03156PB e ARG = 0,28013 + 0,02318PB podem predizer o conteúdo em aminoácidos totais e digestíveis, respectivamente, do milho e seus subprodutos.The digestibility coefficients and thetrue digestible amino acids contents of corn and corn byproducts were determined by using cecectomized adult cockerels. Each one of the 11 feedstuffs evaluated was fed to six cockerels, consuming 15 g at 8a.m. and 15 g at 4p

  14. Resistance to Bt maize in Mythimna unipuncta (Lepidoptera: Noctuidae) is mediated by alteration in Cry1Ab protein activation.

    Science.gov (United States)

    González-Cabrera, Joel; García, Matías; Hernández-Crespo, Pedro; Farinós, Gema P; Ortego, Félix; Castañera, Pedro

    2013-08-01

    Bt maize cultivars based on the event MON810 (expressing Cry1Ab) have shown high efficacy for controlling corn borers. However, their efficiency for controlling some secondary lepidopteran pests such as Mythimna unipuncta has been questioned, raising concerns about potential outbreaks and its economic consequences. We have selected a resistant strain (MR) of M. unipuncta, which is capable of completing its life cycle on Bt maize and displays a similar performance when feeding on both Bt and non-Bt maize. The proteolytic activation of the protoxin and the binding of active toxin to brush border membrane vesicles were investigated in the resistant and a control strain. A reduction in the activity of proteolytic enzymes, which correlates with impaired capacity of midgut extracts to activate the Cry1Ab protoxin has been observed in the resistant strain. Moreover, resistance in larvae of the MR strain was reverted when treated with Cry1Ab toxin activated with midgut juice from the control strain. All these data indicate that resistance in the MR strain is mediated by alteration of toxin activation rather than to an increase in the proteolytic degradation of the protein. By contrast, binding assays performed with biotin labelled Cry1Ab suggest that binding to midgut receptors does not play a major role in the resistance to Bt maize. Our results emphasize the risk of development of resistance in field populations of M. unipuncta and the need to consider this secondary pest in ongoing resistance management programs to avoid the likely negative agronomic and environmental consequences.

  15. Getting domestication straight: ramosa1 in maize.

    Science.gov (United States)

    Dempewolf, Hannes

    2010-04-01

    Knowledge of the identities and characteristics of genes that govern the dramatic phenotypic differences between cultivated plants and their wild ancestors has greatly enhanced our understanding of the domestication process. In this issue of Molecular Ecology, Sigmon & Vollbrecht report the discovery of a new maize domestication gene, ramosa1, which encodes a putative transcription factor in the ramosa developmental pathway. Ramosa1 appears to be instrumental in determining the straightness of kernel rows on the maize cob. The key domestication alleles at ramosa1 are prevalent in landraces of maize. These results reinforce findings from previous studies of crop evolution by highlighting the importance of standing genetic variation and changes in transcriptional regulators in domestication. The evolutionary genetics of domestication also provides a framework for predicting the evolutionary response of organisms to strong human-induced selection pressures over limited time intervals.

  16. MORPHOLOGICAL AND THERMAL PROPERTIES OF MAIZE STARCH

    Directory of Open Access Journals (Sweden)

    Elena Corina Popescu

    2010-01-01

    Full Text Available Maize, rice, wheat and potato are the main sources of starches which differ significantly in composition, morphology,thermal, rheological and retrogradation properties. Starch has unique thermal properties and functionality that havepermitted its wide use in food products and industrial applications.The structure of the starch granule results from the physical arrangement of amylose and amylopectin. Amylose contentof starches from different maize types ranged between 15.3% and 25.1%. Amylopectin is considered responsible for thecrystalline structure of starch granules.The morphological and physicochemical characteristics of maize starch are related to the enzymes involved in itsbiosynthesis.The surface of the starch granule plays a fundamental rôle as the first barrier to processes such as granule hydration,enzyme attack, and chemical reaction with modifying agents. Major parameters describing the solid surface are:specific surface area, total pore volume, mean pore radius (diameter and pore volume distribution in relation to poreradius (diameter.

  17. Corn stalk orientation effect on mechanical cutting

    Energy Technology Data Exchange (ETDEWEB)

    Igathinathane, C. [Mississippi State University (MSU); Womac, A.R. [University of Tennessee; Sokhansanj, Shahabaddine [ORNL

    2010-07-01

    Research efforts that increase the efficiency of size reduction of biomass can lead to a significant energy saving. This paper deals with the determination of the effect of sample orientation with respect to cutting element and quantify the possible cutting energy reduction, utilising dry corn stalks as the test material (15%e20% wet basis). To evaluate the mechanical cutting characteristics of corn stalks, a Warnere Bratzler device was modified by replacing its blunt edged cutting element with one having a 30_ single bevel sharp knife edge. Cutting force-deformation characteristics obtained with a universal testing machine were analysed to evaluate the orientation effects at perpendicular (90o), inclined (45o), and parallel (0o) orientations on internodes and nodes for cutting force, energy, ultimate stress, and specific energy of corn stalks. The corn stalks cutting force-displacement characteristics were found to differ with orientation, and internode and node material difference. Overall, the peak failure force, and the total cutting energy of internodes and nodes varied significantly (P < 0.05) with stalk cross-sectional area. The specific energy values (total energy per unit cut area) of dry corn stalk internodes ranged from 11.3 to 23.5 kN m_1, and nodes from 8.6 to 14.0 kN m_1. The parallel orientation (along grain) compared to perpendicular (across grain) produced a significant reduction of the cutting stress and the specific energy to one tenth or better for internodes, and to about one-fifth for nodes.

  18. Pantoea stewartii subsp. stewartii: lessons learned from a xylem-dwelling pathogen of sweet corn.

    Science.gov (United States)

    Roper, M Caroline

    2011-09-01

    Pantoea stewartii subsp. stewartii is a Gram-negative enteric bacterium that primarily infects sweet corn. Studies of this bacterium have provided useful insight into how xylem-dwelling bacteria establish themselves and incite disease in their hosts. Pantoea stewartii subsp. stewartii is a remarkable bacterial system for laboratory studies because of its relative ease of propagation and genetic manipulation, and the fact that it appears to employ a minimal number of pathogenicity mechanisms. In addition, P. stewartii subsp. stewartii produces copious amounts of its quorum sensing (QS) signal, acyl-homoserine lactone (AHL), making it an excellent organism for studying QS-controlled gene regulation in a plant-pathogenic bacterium. In fact, P. stewartii subsp. stewartii has become the microbial paradigm for QS control of gene expression by both repression and activation via a QS regulator that binds DNA in the absence and dissociates in the presence of the signal ligand. Moreover, P. stewartii subsp. stewartii is a member of the Enterobacteriaceae, and lessons learned from its interaction with plants may be extrapolated to other plant-associated enterics, such as Erwinia, Dickeya and Pectobacterium spp., or enteric human pathogens associated with plants, such as Escherichia coli and Salmonella spp. Bacteria; Gammaproteobacteria; family Enterobacteriaceae; genus Pantoea; species stewartii (Mergaert et al., 1993). Gram-negative, motile, yellow pigmented, mucoid, facultative anaerobe. Pantoea stewartii subsp. stewartii (Smith, 1898) Dye causes Stewart's wilt of corn (Zea mays). Early-maturing sweet corn varieties and some elite inbred maize lines are particularly susceptible. There are two major phases of Stewart's wilt disease: (i) wilt and (ii) leaf blight. The wilt phase occurs when young seedlings are infected with P. stewartii subsp. stewartii (Fig. 1A). Water-soaked lesions first appear on the young expanding leaves and, later, seedlings may become severely wilted

  19. Performance experiment of friction conveying device for maize ears%玉米果穗螺旋摩擦输送装置的输送性能试验

    Institute of Scientific and Technical Information of China (English)

    刘师多; 牛康; 师清翔; 王升升; 袁琦堡; 张奎

    2013-01-01

      At present, chain-rake grain conveying devices are widely used in corn united harvest machines. They are reliable with stable performance, but their structures are complicated. Screw conveying devices have the advantages of being simple in structure;they are also applied in corn united harvest machines, but since they use a helical blade space to transport corn, the structures are huge and difficult to configure in small corn united harvest machines. Therefore, it is necessary to develop a new conveying device. The blades of the new maize ears helical blade friction conveying devices are lower, so the grains during the course of transmission are on the top of the blades at all times. Thus, the conveyer structure can be smaller in size, with less grain injuries in the conveying process.   By using a self-made conveying device for maize ears, the research focused on the laws of effects on maize ears conveying performance caused by the new-type transmission auger’s blade height, guide side panel’s inclining angle, guide side panel position angle, auger lift-and-convey angle, and the rotational speed of the auger. Performances of maize ear conveying devices mainly displays in three aspects:the damage degree of maize ears, the quantity of shattering, and the transport capacity. In the paper, maize ears were first be divided into four states according to the average length, diameter and differences of moisture content. The first state was chosen in the test. Maize ears of the first state are different from the ones of state two, three, and four, respectively, in diameter, length, and moisture content. The state classification was used to study the adaptability of the conveyor as the single characteristic of the material changes. It helps to compare the test results more directly and clearly. Analysis of variance was used in the test to calculate the test results.   The test verified that the maize ear conveying device is adapted to many causes

  20. Digital gene expression signatures for maize development.

    Science.gov (United States)

    Eveland, Andrea L; Satoh-Nagasawa, Namiko; Goldshmidt, Alexander; Meyer, Sandra; Beatty, Mary; Sakai, Hajime; Ware, Doreen; Jackson, David

    2010-11-01

    Genome-wide expression signatures detect specific perturbations in developmental programs and contribute to functional resolution of key regulatory networks. In maize (Zea mays) inflorescences, mutations in the RAMOSA (RA) genes affect the determinacy of axillary meristems and thus alter branching patterns, an important agronomic trait. In this work, we developed and tested a framework for analysis of tag-based, digital gene expression profiles using Illumina's high-throughput sequencing technology and the newly assembled B73 maize reference genome. We also used a mutation in the RA3 gene to identify putative expression signatures specific to stem cell fate in axillary meristem determinacy. The RA3 gene encodes a trehalose-6-phosphate phosphatase and may act at the interface between developmental and metabolic processes. Deep sequencing of digital gene expression libraries, representing three biological replicate ear samples from wild-type and ra3 plants, generated 27 million 20- to 21-nucleotide reads with frequencies spanning 4 orders of magnitude. Unique sequence tags were anchored to 3'-ends of individual transcripts by DpnII and NlaIII digests, which were multiplexed during sequencing. We mapped 86% of nonredundant signature tags to the maize genome, which associated with 37,117 gene models and unannotated regions of expression. In total, 66% of genes were detected by at least nine reads in immature maize ears. We used comparative genomics to leverage existing information from Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) in functional analyses of differentially expressed maize genes. Results from this study provide a basis for the analysis of short-read expression data in maize and resolved specific expression signatures that will help define mechanisms of action for the RA3 gene.