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Sample records for coriander coriandrum sativum

  1. Essential oil and extract of coriander (Coriandrum sativum L.

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    Zeković Zoran

    2011-01-01

    Full Text Available Two different methods of coriander (Coriandrum sativum L. essential oil isolation, steam distillation and extraction by methylene chloride (Soxhlet extraction were investigated. After the determination of essential oil content in the investigated drug and in dry extract (using steam distillation, qualitative and quantitative composition of obtained essential oils, determined by TLC and GC-MS methods, were compared. The content of linalool was higher (52.4% in essential oil obtained by coriander steam distillation than that in essential oil separeted from dry extract (42.8%, and, on the other hand, content of geranyl-acetate was lower (4.6% and 11.7%, respectively.

  2. Coriander (Coriandrum sativum L.) and its bioactive constituents.

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    Laribi, Bochra; Kouki, Karima; M'Hamdi, Mahmoud; Bettaieb, Taoufik

    2015-06-01

    Coriander (Coriandrum sativum L.), a member of the Apiaceae family, is among most widely used medicinal plant, possessing nutritional as well as medicinal properties. Thus, the aim of this updated review is to highlight the importance of coriander as a potential source of bioactive constituents and to summarize their biological activities as well as their different applications from data obtained in recent literature, with critical analysis on the gaps and potential for future investigations. A literature review was carried out by searching on the electronic databases including PubMed, Scopus, ScienceDirect, and Google Scholar for studies focusing on the biological and pharmacological activities of coriander seed and herb bioactive constituents. All recent English-language articles published between 2000 and 2014 were searched using the terms 'C. sativum', 'medicinal plant', 'bioactive constituents', and 'biological activities'. Subsequently, coriander seed and herb essential oils have been actively investigated for their chemical composition and biological activities including antimicrobial, antioxidant, hypoglycemic, hypolipidemic, anxiolytic, analgesic, anti-inflammatory, anti-convulsant and anti-cancer activities, among others. Although coriander has been reported to possess a wide range of traditional medicinal uses, no report is available in its effectiveness use in reactive airway diseases such as asthma and bronchiolitis. In brief, the information presented herein will be helpful to create more interest towards this medicinal species by defining novel pharmacological and clinical applications and hence, may be useful in developing new drug formulations in the future or by employing coriander bioactive constituents in combination with conventional drugs to enhance the treatment of diseases such as Alzheimer and cancer.

  3. Hydroponic Phytoremediation of Nickel by Coriander (Coriandrum sativum

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    Mozhgan Tagharobiyan

    2015-09-01

    Full Text Available Environmental stresses are one of the most important factors of agricultural products reduction in the world. The influence of different concentrations of nickel nitrate (0, 100, 200 and 500 μM in Hoagland’s solution on dry matter, catalase enzyme, flavonoids, hydrogen peroxide, peroxidase Enzyme, MDA and accumulation of Ni were studied in coriander (Coriandrum sativum plants. Treatment with Ni led to significant increase in flavonoids, hydrogen peroxide, MDA and other aldehyde. Exposure of coriander plant to Ni altered catalase enzymes, leading to significant decrease in their contents. In both shoots and roots of coriander plants, significant decrease in dry matter was observed. Ni accumulation increased significantly in shoots and roots. Ni increased in the roots more than the shoots. According to a more accumulation of Ni in the roots, the expansion of plants root can help to better adaptability with the toxicity of metals. It may be used as an indicator to illustrate the differences between plant species.

  4. Microwave drying characteristics of coriander (Coriandrum sativum L.) leaves

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    Sarimeseli, Ayse [Department of Chemical Engineering, Inonu University, 44069 Malatya (Turkey)

    2011-02-15

    Effect of microwave power output on effective moisture diffusivity, colour parameters and rehydration characteristics of coriander leaves (Coriandrum sativum L.) was investigated by using a microwave drier. Within the range of microwave power values, 180-360 W, effective moisture diffusivities were found to be 6.3 x 10{sup -11}-2.19 x 10{sup -10} m{sup 2}/s and the result could successfully be presented with the model suggested by Midilli et al. No significant differences in the colour parameters were obtained between the fresh and dried samples and the changes in their values were not dependent on the power outputs of the microwave drier. The highest rehydration capacity was recorded for the samples dried at 180 W and lowest at 900 W. (author)

  5. Effects of steam distillation on extraction, composition, and functional properties of coriander (Coriandrum sativum L.) proteins

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    Coriander (Coriandrum sativum L.) is a summer annual plant commonly used as fresh green herb, spice, or for its essential oil. A newly-developed process combined steam distillation and mechanical pressing to recover the essential oil and edible oil, respectively, from dehulled coriander seeds. The c...

  6. Coriander (Coriandrum sativum L. essential oil: Chemistry and biological activity

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    Shyamapada Mandal

    2015-06-01

    Full Text Available Coriandrum sativum L. (C. sativum is one of the most useful essential oil bearing spices as well as medicinal plants, belonging to the family Umbelliferae/Apiaceae. The leaves and seeds of the plant are widely used in folk medicine in addition to its use as a seasoning in food preparation. The C. sativum essential oil and extracts possess promising antibacterial, antifungal and anti-oxidative activities as various chemical components in different parts of the plant, which thus play a great role in maintaining the shelf-life of foods by preventing their spoilage. This edible plant is non-toxic to humans, and the C. sativum essential oil is thus used in different ways, viz., in foods (like flavouring and preservatives and in pharmaceutical products (therapeutic action as well as in perfumes (fragancias and lotions. The current updates on the usefulness of the plant C. sativum are due to scientific research published in different web-based journals.

  7. Safety assessment of coriander (Coriandrum sativum L.) essential oil as a food ingredient.

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    Burdock, George A; Carabin, Ioana G

    2009-01-01

    Coriander essential oil is used as a flavor ingredient, but it also has a long history as a traditional medicine. It is obtained by steam distillation of the dried fully ripe fruits (seeds) of Coriandrum sativum L. The oil is a colorless or pale yellow liquid with a characteristic odor and mild, sweet, warm and aromatic flavor; linalool is the major constituent (approximately 70%). Based on the results of a 28 day oral gavage study in rats, a NOEL for coriander oil is approximately 160 mg/kg/day. In a developmental toxicity study, the maternal NOAEL of coriander oil was 250 mg/kg/day and the developmental NOAEL was 500 mg/kg/day. Coriander oil is not clastogenic, but results of mutagenicity studies for the spice and some extracts are mixed; linalool is non-mutagenic. Coriander oil has broad-spectrum, antimicrobial activity. Coriander oil is irritating to rabbits, but not humans; it is not a sensitizer, although the whole spice may be. Based on the history of consumption of coriander oil without reported adverse effects, lack of its toxicity in limited studies and lack of toxicity of its major constituent, linalool, the use of coriander oil as an added food ingredient is considered safe at present levels of use.

  8. The cholesterol lowering property of coriander seeds (Coriandrum sativum): mechanism of action.

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    Dhanapakiam, P; Joseph, J Mini; Ramaswamy, V K; Moorthi, M; Kumar, A Senthil

    2008-01-01

    Coriandrum sativum (Coriander) has been documented as a traditional treatment for cholesterol and diabetes patients. In the present study, coriander seeds incorporated into diet and the effect of the administration of coriander seeds on the metabolism of lipids was studied in rats, fed with high fat diet and added cholesterol. The seeds had a significant hypolipidemic action. In the experimental group of rats (tissue) the level of total cholesterol and triglycerides increased significantly There was significant increase in beta-hydroxy, beta-methyl glutaryl CoA reductase and plasma lecithin cholesterol acyl transferase activity were noted in the experimental group. The level of low density lipoprotein (LDL) + very low density lipoprotein (VLDL) cholesterol decreased while that of high density lipoprotein (HDL) cholesterol increased in the experimental group compared to the control group. The increased activity of plasma LCAT enhanced degradation of cholesterol to fecal bile acids and neutral sterols appeared to account for its hypocholesterolemic effect.

  9. Essential oil composition of the coriander (Coriandrum sativum L. herb depending on the development stage

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    Renata Nurzyńska-Wierdak

    2013-04-01

    Full Text Available The herbal material of Coriandrum sativum is the fruit. Fresh herb is also used as an aromatic spice. The aim of the present study was to evaluate the content and chemical composition of coriander herb obtained at different plant growth stages. Coriander plants were grown in a glasshouse, the herb was harvested at the initial stage of flowering and from regrowing shoots. Essential oil extraction from the herb was performed by the hydrodistillation method, whereas the assessment of the chemical composition – using GC-MS method. The examined material contained 0.17–0.29 ml × 100g-1 of essential oil, depending on the stage of plant development when the harvest was done. 61 (generative phase and 65 (vegetative phase compounds were found in the examined coriander oil. The essential oil from the coriander herb contained the highest amount of aliphatic aldehydes, among which was decanal, E-2-dodecanol and E-2-decenol had the highest percentages. The contents of most aliphatic aldehydes decreased with each subsequent harvest of the herb. In addition to the above-mentioned aliphatic aldehydes, the presence of linalool, phytol, and oleic acid was found in the essential oil extracted from the coriander herb.

  10. Coriander (Coriandrum sativum L.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry.

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    Silva, Filomena; Ferreira, Susana; Queiroz, João A; Domingues, Fernanda C

    2011-10-01

    The aim of this work was to study the antibacterial effect of coriander (Coriandrum sativum) essential oil against Gram-positive and Gram-negative bacteria. Antibacterial susceptibility was evaluated using classical microbiological techniques concomitantly with the use of flow cytometry for the evaluation of cellular physiology. Our results showed that coriander oil has an effective antimicrobial activity against all bacteria tested. Also, coriander oil exhibited bactericidal activity against almost all bacteria tested, with the exception of Bacillus cereus and Enterococcus faecalis. Propidium iodide incorporation and concomitant loss of all other cellular functions such as efflux activity, respiratory activity and membrane potential seem to suggest that the primary mechanism of action of coriander oil is membrane damage, which leads to cell death. The results obtained herein further encourage the use of coriander oil in antibacterial formulations due to the fact that coriander oil effectively kills pathogenic bacteria related to foodborne diseases and hospital infections.

  11. Prophylactic efficacy of Coriandrum sativum (Coriander) on testis of lead-exposed mice.

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    Sharma, Veena; Kansal, Leena; Sharma, Arti

    2010-09-01

    Lead poisoning is a worldwide health problem, and its treatment is under investigation. The aim of this study was to access the efficacy of Coriandrum sativum (coriander) in reducing lead-induced changes in mice testis. Animal exposed to lead nitrate showed significant decrease in testicular SOD, CAT, GSH, total protein, and tissue lead level. This was accompanied by simultaneous increase in the activities of LPO, AST, ALT, ACP, ALP, and cholesterol level. Serum testosterone level and sperm density were suppressed in lead-treated group compared with the control. These influences of lead were prevented by concurrent daily administration of C. sativum extracts to some extent. Treating albino mice with lead-induced various histological changes in the testis and treatment with coriander led to an improvement in the histological testis picture. The results thus led us to conclude that administration of C. sativum significantly protects against lead-induced oxidative stress. Further work need to be done to isolate and purify the active principle involved in the antioxidant activity of this plant.

  12. Antimutagenicity of coriander (Coriandrum sativum) juice on the mutagenesis produced by plant metabolites of aromatic amines.

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    Cortés-Eslava, Josefina; Gómez-Arroyo, Sandra; Villalobos-Pietrini, Rafael; Espinosa-Aguirre, Jesús Javier

    2004-11-02

    Aromatic amines are metabolically activated into mutagenic compounds by both animal and plant systems. The 4-nitro-o-phenylenediamine (NOP) is a well-known direct-acting mutagen whose mutagenic potential can be enhanced by plant metabolism; m-phenylenediamine (m-PDA) is converted to mutagenic products detected by the Salmonella typhimurium TA98 strain, and 2-aminofluorene (2-AF) is the plant-activated promutagen most extensively studied. Plant cells activate both 2-AF and m-PDA into potent mutagens producing DNA frameshift mutations. Coriander (Coriandrum sativum) is a common plant included in the Mexican diet, usually consumed uncooked. The antimutagenic activity of coriander juice against the mutagenic activity of 4-nitro-o-phenylenediamine, m-phenylenediamine and 2-aminofluorene was investigated using the Ames reversion mutagenicity assay (his- to his+) with the S. typhimurium TA98 strain as indicator organism. The plant cell/microbe coincubation assay was used as the activating system for aromatic transformation and plant extract interaction. Aqueous crude coriander juice significantly decreased the mutagenicity of metabolized aromatic amines (AA) in the following order: 2-AF (92.43%) > m-PDA (87.14%) > NOP (83.21%). The chlorophyll content in vegetable juice was monitored and its concentration showed a positive correlation with the detected antimutagenic effect. Protein content and peroxidase activity were also determined. The concentration of coriander juice (50-1000 microl/coincubation flask) was neither toxic nor mutagenic. The similar shape of the antimutagenic response curves obtained with coriander juice and chlorophyllin (used as a subrogate molecule of chlorophyll) indicated that comparable mechanisms of mutagenic inhibition could be involved. The negative correlation between chlorophyll content and mutagenic response of the promutagenic and direct-acting used amines allows us to deduce that a chemical interaction takes place between the two molecules

  13. Effect of NaCl priming on seed germination of four coriander cultivars (Coriandrum sativum

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    Mariem Ben Fredj

    2013-04-01

    Full Text Available Background: Optimum benefits of seed priming on four cultivars of coriander (Coriandrum sativum having different origin (Tunisia, Algeria, Egypt, and Syria were studied under salinity stress. Materials and Methods: Seeds of coriander were primed with NaCl at five levels (0, 2, 4, 6 and 8 g L-1 for three different times (12, 24 and 36 h at 25°C. Then primed (P and non-primed (NP seeds were irrigated with five different saline solutions consisting of 0 (control, 2, 4, 6 and 8 g L-1 NaCl. Results: The results showed that the best germination percentage was obtained by applying NaCl at 4 g L-1 for 12 h. Also, NaCl priming increased germination percentage compared with non-primed seeds. Overall increased NaCl level led to the reductions in the traits under study but these reductions were higher for non-primed compared to primed seeds. An Egyptian cultivar seemed to be the most sensitive. Conclusions: The present study revealed that under salinity seed priming with NaCl could be used as a method to improve seed performance in coriander. However, further studies are needed to investigate the effects of NaCl priming on later growth and development of this crop.

  14. Atividade antioxidante de extratos de coentro (Coriandrum sativum L. Antioxidant activity of coriander extracts (Coriandrum sativum L.

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    Enayde de Almeida Melo

    2003-12-01

    Full Text Available A atividade antioxidante de diferentes extratos de coentro (Coriandrum sativum L., isolados, associados entre si e com o BHT foi investigada. A ação antioxidante, exercida pelos extratos etéreo, etanólico e aquoso, obtidos por processo de extração seqüencial, foi avaliada através de sistema modelo b-caroteno/ácido linoléico e os compostos responsáveis por esta ação identificados. O efeito sinergista entre os extratos aquoso e etéreo foi avaliado utilizado o planejamento fatorial 2 ² . Os extratos aquoso, etéreo e etanólico exibiram 69,83%, 61,89% e 40,50%, respectivamente, de proteção contra a oxidação. Compostos fenólicos foram detectados nos dois primeiros extratos e constatada a presença de carotenóides no etéreo. Ao combinar os dois extratos, em diferentes concentrações, o percentual de inibição da oxidação foi inferior ao dos extratos isolados, demonstrando não haver sinergismo entre eles. Associações de diferentes concentrações de BHT com o extrato aquoso exibiram elevada ação antioxidante, enquanto com o extrato etéreo esta ação foi levemente superior a do extrato isolado. A habilidade dos extratos aquoso e etéreo em retardar a oxidação pode ser atribuída, respectivamente, aos seus constituintes fenólicos e carotenóides. O extrato aquoso pode ser considerado como um potencial antioxidante, cuja ação pode ser intensificada ao ser empregado juntamente com BHT.The antioxidant activity of different extracts of coriander (Coriandrum sativum, isolated and/or combined with itself and BHT, was investigated. The etheric, ethanolic and aqueous extracts obtained by sequential extraction were evaluated for antioxidant action in a b-carotene/acid linoléico model system and the active compounds identified. The antioxidant synergistic effect between aqueous and etheric extracts was evaluated using factorial 2² plan. The antioxidant action of the extracts aqueous, etheric and ethanolic was 69.83%, 61

  15. Crayfish chitosan for microencapsulation of coriander (Coriandrum sativum L.) essential oil.

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    Duman, Fatih; Kaya, Murat

    2016-11-01

    In this study, chitosan, which was obtained from the waste shells of crayfish (Astacus leptodactylus), was used for the encapsulation of the essential oil isolated from coriander (Coriandrum sativum L.) via the spray drying method. The obtained capsules were characterized using SEM, FT-IR, TGA and XRD. The size of the microcapsules was between 400nm - 7μm. It was determined that the swelling characteristic of the capsules was pH sensitive. The release showed bi-phasic characteristics and the maximum degree was reached after 72h. Antimicrobial activity studies showed that pure chitosan more effective than the capsule. The antioxidant activity was recorded concentration-dependent. In contrast the antimicrobial activity, antioxidant activity of the capsule was found much higher than the oil and the pure chitosan. Consequently, it was determined that this product could be used in the food and pharmaceutical industries as a natural antioxidant and antimicrobial agent.

  16. Picosecond nonlinear optical studies of gold nanoparticles synthesised using coriander leaves (Coriandrum sativum)

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    Venugopal Rao, S.

    2011-07-01

    The results are presented from the experimental picosecond nonlinear optical (NLO) studies of gold nanoparticles synthesised using coriander leaf (Coriandrum sativum) extract. Nanoparticles with an average size of ∼30 nm (distribution of 5-70 nm) were synthesised according to the procedure reported by Narayanan et al. [Mater. Lett. 2008, 62, 4588-4591]. NLO studies were carried out using the Z-scan technique using 2 ps pulses near 800 nm. Open-aperture data suggested saturation absorption as the nonlinear absorption mechanism, whereas closed-aperture data suggested a positive nonlinearity. The magnitude of third-order nonlinearity was estimated to be (3.3 ± 0.6) × 10-13 esu. A solvent contribution to the nonlinearity was also identified and estimated. A comparison is attempted with some recently reported NLO studies of similar gold nanostructures.

  17. Effects of steam distillation and screw-pressing on extraction, composition and functional properties of protein in dehulled coriander (Coriandrum sativum L.)

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    Coriander (Coriandrum sativum L.) is a summer annual plant commonly used as fresh green herb, spice, or for its essential oil. An integrated process combined steam distillation, dehulling, and screw pressing to recover the essential oil and edible oil from coriander fruit. The current work determine...

  18. Immunotoxicity activity from the essential oils of coriander (Coriandrum sativum) seeds.

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    Chung, Ill-Min; Ahmad, Ateeque; Kim, Eun-Hye; Kim, Seung-Hyun; Jung, Woo-Suk; Kim, Jin-Hoi; Nayeem, Abdul; Nagella, Praveen

    2012-06-01

    The seeds of the Coriandrum sativum were extracted and the essential oil composition and immunotoxicity effects were studied. The analysis of the essential oil was conducted by gas chromatography-mass spectroscopy, which revealed 33 components, representing 99.99% of the total oil from the seeds of coriander. The major components are linalool (55.09%), α-pinene (7.49%), 2,6-Octadien-1-ol, 3,7-dimethyl-, acetate, (E)- (5.70%), geraniol (4.83%), 3-Cyclohexene-1-methanol, α,α,4-trimethyl- (4.72%), hexadecanoic acid (2.65%), tetradecanoic acid (2.49%), 2-α-pinene (2.39%), citronellyl acetate (1.77%), and undecanal (1.29%). The seed oil had significant toxic effects against the larvae of Aedes aegypti with an LC(50) value of 21.55 ppm and LC(90) value of 38.79 ppm. The above data indicate that the major components in the essential oil of coriander play an important role as immunotoxicity on the A. aegypti.

  19. Gamma radiation effects in coriander (coriandrum sativum L) for consumption in Mexico

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    Cruz Z, E. [UNAM, Instituto de Ciencias Nucleares, Unidad de Irradiacion y Seguridad Radiologica, Apdo. Postal 70-543, 04510 Mexico D. F. (Mexico); Ruiz G, B. [Universidad de Sonora, Departmento de Agricultura y Ganaderia, Apdo. Postal 305, 83190 Hermosillo, Sonora (Mexico); Flores E, T. [UNAM, Facultad de Quimica, Departamento de Alimentos y Biotecnologia, Ciudad Universitaria, 04510 Mexico D. F. (Mexico); Barboza F, M. [Universidad de Sonora, Centro de Investigacion en Fisica, Apdo. Postal 5-088, 83190 Hermosillo, Sonora (Mexico)

    2011-02-15

    Ionizing radiation is an effective process for disinfecting and prolonging the shelf-life of several food products. Food irradiation may be one of the most significant contributions to public health in the developing countries. Following the irradiation it is necessary to analyze the radiation dose effects in foodstuffs. Thermally stimulated luminescence (Tl) properties and microbiological load as a function of the gamma doses were analyzed in fresh commercial Coriander (Coriandrum sativum L.) samples. For Tl analyses the poly mineral fraction was separated from a coriander and 10 {mu}m size particles were selected. The poly mineral samples were exposed to a 0.5-15,000 Gy dose from gamma radiation using a {sup 60}Co facility, Gamma beam 651PT, semi-industrial irradiator with 98.4 Gy/min dose rate. The glow curves were broad bands and characteristic of quartz that is present in the sample as detected by XRD. The main Tl characteristics were determined, including the structure of the glow curves, Tl response, reproducibility of Tl signals over 12 cycles of subsequent irradiations, and the fading effect during the storage during 30 days. The Tl method was found useful for detection of irradiated coriander. In order to analyze the effect of gamma radiation on the bacterial load in the fresh food coriander, several coriander samples were exposed to 0-10 kGy dose. It was observed that at 0.5 kGy dose the aerobic mesophilic count was reduced to 99.9%, while the initial total coliform bacteria decreased from 871,000 cfu/g to less than 100. The microbiological results are lower than the limit indicated by the Mexican regulatory authority; 150,000 cfu/g for mesophiles and 100 cfu/g for total coliforms. The aim of this work is to investigate the Tl properties of the poly mineral fraction obtained from coriander and to measure the microbiological load as a function of the gamma irradiation dose also. (Author)

  20. Genetic divergence and its implication in breeding of desired plant type in coriander -Coriandrum sativum L.-

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    Singh S.P.

    2005-01-01

    Full Text Available Seventy germplasm lines of coriander (Coriandrum sativum L. of diverse eco-geographical origin were undertaken in present investigation to determine the genetic divergence following multivariate and canonical analysis for seed yield and its 9 component traits. The 70 genotypes were grouped into 9 clusters depending upon the genetic architecture of genotypes and characters uniformity and confirmed by canonical analysis. Seventy percent of total genotypes (49/70 were grouped in 4 clusters (V, VI, VIII and IX, while apparent diversity was noticed for 30 percent genotypes (21/70 that diverged into 5 clusters (I, II, III, FV, and VII. The maximum inter cluster distance was between I and IV (96.20 followed by III and IV (91.13 and I and VII (87.15. The cluster VI was very unique having genotypes of high mean values for most of the component traits. The cluster VII had highest seeds/umbel (35.3 ± 2.24, and leaves/plant (12.93 ± 0.55, earliest flowering (65.05 ± 1.30 and moderately high mean values for other characters. Considering high mean and inter cluster distance breeding plan has been discussed to select desirable plant types.

  1. Detection and thermoluminescence of coriander (Coriandrum sativum L.) irradiated with {sup 60}Co; Deteccion y termoluminiscencia del cilantro (Coriandrum sativum L.) irradiado con {sup 60}Co

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    Ruiz G, B. [Departamento de Agricultura y Ganaderia, Universidad de Sonora, 083190 Hermosillo, Sonora (Mexico); Cruz Z, E. [Unidad de Irradiacion y Seguridad Radiologica, Instituto de Ciencias Nucleares, UNAM, A. P. 70-543, 04510 Mexico D. F. (Mexico); Barboza F, M. [Centro de Investigacion en Fisica, Universidad de Sonora, A. P. 5-088, 083190 Hermosillo, Sonora (Mexico)

    2009-10-15

    The fresh and dried coriander (Coriandrum sativum L.) is a spice of high commercial value as food for consumption in Mexico. Their hygienic quality is often poor due to some factors: contaminants present in irrigation water and the cultivation atmosphere, harvesting and post harvest handling, mainly. The process by radiation is an alternative to achieve the sterilization to adequate dose for the hygienic quality for its consumption. However, irradiation also involves food detection exposed to radiation. This paper presents detected results on the dried coriander that was obtained from fresh samples and thermoluminescent properties such as glow curves structure from low doses (0.5 Gy) to high (15 kGy), the dose response, thermoluminescent signal decay, in order to determine the loss of stability during the storage of the poly mineral fraction. We obtained the inorganic fraction separation of the organic part of particle sizes of 10 {mu}m by the Zimmerman method. The samples were exposed at two dose ranges; 0.5-400 Gy and 0.5-15 kGy of gamma radiation with {sup 60}Co. The limit of thermoluminescent detection of the irradiated coriander samples was from 1 Gy. The glow curves were at a broad band of 35-400 C, with a maximum thermoluminescence around 182-196 C, and run at 164 C for high doses of 15 kGy. The range of linear response to dose was 4-25 Gy, whereas higher doses than the kGy order the response increase with the dose. The thermoluminescent properties of the coriander poly mineral fraction, show it can be used to identify irradiated food at gamma doses relatively low and even in doses of commercial interest due to the high stability of thermoluminescent signals. (Author)

  2. Metabolic activation of three arylamines and two organophosphorus insecticides by coriander (Coriandrum sativum) a common edible vegetable.

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    Cortés-Eslava, J; Gómez-Arroyo, S; Villalobos-Pietrini, R; Espinosa-Aguirre, J J

    2001-12-15

    Organophosphorus insecticides and arylamines, widely distributed in the environment, can be activated into mutagens by plants. Plant activation of three aromatic amines, 4-nitro-o-phenylenediamine (NOP), m-phenylenediamine (m-PDA) and 2-aminofluorene (2AF), and two organophosphorus insecticides, dimethoate and methyl parathion has been the focus of this study. The plant cell/microbe coincubation assay was used employing coriander (Coriandrum sativum) suspended cell cultures as the activating system. Interestingly, this vegetable is included in the Mexican diet and ingested generally uncooked and could have epidemiological consequences. As a genetic end point, the Salmonella typhimurium tester strain TA98 was used. Protein contents, as well as peroxidase activity and peroxidase activity inhibited by diethyldithiocarbamate (DEDTC) of coriander cultures were determined after the coculture. Coriander cells highly activated three aromatic amines, NOP, m-PDA and 2-AF to mutagenic products detected in Salmonella. On the other hand, insecticides were only lightly activated, probably because peroxidase activity of coriander cells was inhibited, corroborated by DEDTC peroxidase inhibition. In all the assays, NOP was the more potent mutagenic compound. The results demonstrated that coriander cells were metabolically competent and suitable for a plant cell microbe coincubation assay, developed to analyze the promutagen activation by plant systems and can be used as a indicator of potential genetic effects.

  3. Comparison of Different Extraction Methods for the Determination of Essential oils and Related Compounds from Coriander (Coriandrum sativum L.).

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    Msaada, Kamel; Taârit, Mouna Ben; Hosni, Karim; Nidhal, Salem; Tammar, Sonia; Bettaieb, Iness; Hammami, Mohamed; Limam, Férid; Marzouk, Brahim

    2012-12-01

    The volatile oil of coriander (Coriandrum sativum L.) obtained from the fruits by soxhlet-dynamic headspace (S-DHS), solvent extraction (SE), steam distillation (SD), hydrodistillation (HYD) and supercritical CO2 extraction (SC-CO2) were analyzed by GC-FID and GC-MS. The SC-CO2 offered a higher yield (4.5%, w/w) than the other used techniques. Among the identified constituents, linalool was the main compound whatever the employed extraction procedure in contrast to the remaining components which varied according to the isolation technique showing a strong effect of the method used on the composition of these minor compounds. SC-CO2 as compared to the other extraction techniques revealed its high efficiency in addition to the integrity saving of coriander fruit volatiles. Statistical analysis showed that all the detected and identified compounds were highly (P > 0.001) affected by the extraction technique used except the a-terpineol which appear stable. On the other hand, principal component analysis (PCA) revealed on the determination of one group represented by SC-CO2, S-DHS and HYD suggesting a similar essential oil composition. Obtained results show that, in Tunisian coriander essential oil, linalool was the main compound.

  4. Salicylic acid-induced elicitation of folates in coriander (Coriandrum sativum L.) improves bioaccessibility and reduces pro-oxidant status.

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    Puthusseri, Bijesh; Divya, Peethambaran; Lokesh, Veeresh; Neelwarne, Bhagyalakshmi

    2013-01-15

    Foliage of Coriandrum sativum is a rich source of natural folates amenable for enhancement through salicylic acid-mediated elicitation, thereby holding a great promise for natural-mode alleviation of this vitamin (B(9)) deficiency. In the present study we report salicylic acid-mediated differential elicitation of different forms of folates - 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and 10-formyltetrahydrofolate - their stabilities during microwave-drying and bioaccessibilities from fresh and dried foliage. The first two compounds nearly doubled and the third increased sixfold post-elicitation, with all three showing concomitant increase in bioaccessibilities. Although a slight decrease in bioaccessibility was observed in dried foliage, over twofold increase of each form of folate upon elicitation would deliver much higher levels of natural folates from this traditional culinary foliage, which is widely used in many cuisines. Elicitor-mediated folate enhancement also imparted reduction of oxidative status and the enhancement of antioxidant enzyme activities in coriander foliage.

  5. Nematicidal activity of plant essential oils and components from coriander (Coriandrum sativum), Oriental sweetgum (Liquidambar orientalis), and valerian (Valeriana wallichii) essential oils against pine wood nematode (Bursaphelenchus xylophilus).

    Science.gov (United States)

    Kim, Junheon; Seo, Sun-Mi; Lee, Sang-Gil; Shin, Sang-Chul; Park, Il-Kwon

    2008-08-27

    Commercial essential oils from 28 plant species were tested for their nematicidal activities against the pine wood nematode, Bursaphelenchus xylophilus. Good nematicidal activity against B. xylophilus was achieved with essential oils of coriander (Coriandrum sativum), Oriental sweetgum (Liquidambar orientalis), and valerian (Valeriana wallichii). Analysis by gas chromatography-mass spectrometry led to the identification of 26, 11, and 4 major compounds from coriander (Coriandrum sativum), Oriental sweetgum (Liquidambar orientalis), and valerian (Valeriana wallichii) oils, respectively. Compounds from each plant essential oil were tested individually for their nematicidal activities against the pine wood nematode. Among the compounds, benzaldehyde, trans-cinnamyl alcohol, cis-asarone, octanal, nonanal, decanal, trans-2-decenal, undecanal, dodecanal, decanol, and trans-2-decen-1-ol showed strong nematicidal activity. The essential oils described herein merit further study as potential nematicides against the pine wood nematode.

  6. Coriander(Coriandrum sativum L.) essential oil: chemistry and biological activity

    Institute of Scientific and Technical Information of China (English)

    Shyamapada; Mandal; Manisha; Mandal

    2015-01-01

    Coriandrum sativum L.(C. sativum) is one of the most useful essential oil bearing spices as well as medicinal plants, belonging to the family Umbelliferae/Apiaceae. The leaves and seeds of the plant are widely used in folk medicine in addition to its use as a seasoning in food preparation. The C. sativum essential oil and extracts possess promising antibacterial, antifungal and anti-oxidative activities as various chemical components in dif erent parts of the plant, which thus play a great role in maintaining the shelf-life of foods by preventing their spoilage. This edible plant is non-toxic to humans, and the C. sativum essential oil is thus used in dif erent ways, viz., in foods(like l avoring and preservatives) and in pharmaceutical products(therapeutic action) as well as in perfumes(fragancias and lotions). The current updates on the usefulness of the plant C. sativum are due to scientii c research published in dif erent web-based journals.

  7. Effect of water stress and harvesting stages on quantitative and qualitative yields of coriander (Coriandrum sativum L.

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    A. Ahmadian

    2016-05-01

    Full Text Available In order to study the effect of drought stress and harvesting stages on quantitative and qualitative yields of Coriander (Coriandrum sativum L. this experiment was conducted on split plot based on a randomized completely block design with 3 replications in Torbat-e Heydariyeh University, Iran, during growing season of 2010-2011. Treatments were drought stress (in three levels no stress: control and irrigation in 60 and 30 percentage of FC as main plots and harvesting times (in 3 levels consist of: before flowering, flowering and after flowering as sub plots. Results showed that drought stress and harvesting stages had significant affected on leaf number, height, number of stem, wet and dry weight of plant, SPAD, proline content, carbohydrate content, essential oil yield and percentage and components of essential oil of coriander. Increasing water stress decreased yield and its components while enhanced proline and carbohydrate contents. Maximum of essential oil and its main components (linalool, alpha pentene, gamma terpinene, geranial acetate and camphor were in low stress that had significant difference with other stress treatments. Delaying in harvest enhanced yield and its components and essential oil percentage. Proline content had no significant difference between flowering and after flowering stages. Therefore, we can suggest low stress of water and harvest at after flowering stage to get maximum of yield.

  8. Coriander (Coriandrum sativum L.): a potential source of high-value components for functional foods and nutraceuticals--a review.

    Science.gov (United States)

    Sahib, Najla Gooda; Anwar, Farooq; Gilani, Anwarul-Hassan; Hamid, Azizah Abdul; Saari, Nazamid; Alkharfy, Khalid M

    2013-10-01

    Coriander (Coriandrum sativum L.), a herbal plant, belonging to the family Apiceae, is valued for its culinary and medicinal uses. All parts of this herb are in use as flavoring agent and/or as traditional remedies for the treatment of different disorders in the folk medicine systems of different civilizations. The plant is a potential source of lipids (rich in petroselinic acid) and an essential oil (high in linalool) isolated from the seeds and the aerial parts. Due to the presence of a multitude of bioactives, a wide array of pharmacological activities have been ascribed to different parts of this herb, which include anti-microbial, anti-oxidant, anti-diabetic, anxiolytic, anti-epileptic, anti-depressant, anti-mutagenic, anti-inflammatory, anti-dyslipidemic, anti-hypertensive, neuro-protective and diuretic. Interestingly, coriander also possessed lead-detoxifying potential. This review focuses on the medicinal uses, detailed phytochemistry, and the biological activities of this valuable herb to explore its potential uses as a functional food for the nutraceutical industry.

  9. Antioxidant and hepatoprotective potential of essential oils of coriander (Coriandrum sativum L.) and caraway (Carum carvi L.) (Apiaceae).

    Science.gov (United States)

    Samojlik, Isidora; Lakić, Neda; Mimica-Dukić, Neda; Daković-Svajcer, Kornelia; Bozin, Biljana

    2010-08-11

    Essential oils of Coriandrum sativum L. and Carum carvi L. fruits were analyzed by gas chromatography-mass spectrometry and assayed for their in vitro and in vivo antioxidant activity and hepatoprotective effect against carbon tetrachloride (CCl(4)) damage. The in vitro antioxidant activity was evaluated as a free radical scavenging capacity (RSC), measured as scavenging activity of the essential oils on 2,2-diphenyl-1-picrylhydrazyl (DPPH(*)) and OH radicals and effects on lipid peroxidation (LP) in two systems of induction. Some liver biochemical parameters were determined in animals pretreated with essential oils and later intoxicated with CCl(4) to assess in vivo hepatoprotective effect. Tested essential oils were able to reduce the stable DPPH(*) in a dose-dependent manner and to neutralize H(2)O(2), reaching 50% neutralization with IC(50) values of <2.5 microL/mL for Carvi aetheroleum and 4.05 microL/mL for Coriandri aetheroleum . Caraway essential oil strongly inhibited LP in both systems of induction, whereas coriander essential oil exhibited prooxidant activity. In vivo investigation conferred leak of antioxidative capacity of coriander essential oil, whereas the essential oil of caraway appeared promising for safe use in folk medicine and the pharmaceutical and food industries.

  10. Enhancement of folate content and its stability using food grade elicitors in coriander (Coriandrum sativum L.).

    Science.gov (United States)

    Puthusseri, Bijesh; Divya, Peethambaran; Lokesh, Veeresh; Neelwarne, Bhagyalakshmi

    2012-06-01

    Folate (vitamin B₉) content was evaluated in 10 varieties of coriander with the aim of enhancing its concentration and stability, because of three reasons: 1) coriander is among a few widely used greens in the world and suits many cuisines, 2) folate deficiency is prevalent in developing countries causing anaemia, infant mortality and neural tube closure defects, and 3) natural folate is preferred due to doubts about health risks associated with the synthetic form. In C. sativum, the highest folate content of 1,577 μg/100 g DW was found in var. GS4 Multicut foliage of mature plants (marketable stage) with an insignificantly higher content (1,599.74 μg/100 g DW) at flowering, which is a stage not preferred in markets. In callus cultures treated with plant growth regulators (GRs) (6-benzylaminopurine, kinetin and abscisic acid) substantial increase in folate occurred after 6 h, whereas elicitors (methyl jasmonate and salicylic acid) caused rapid 2-fold increase of folate, particularly in response to salicylic acid. Based on these observations, foliar applications were done for in vivo plants, where salicylic acid (250 μM, 24 h) also enhanced folate level by 2-folds (3,112.33 μg/100 g DW), although the content varied with diurnal rhythms. Stability of folates in treated coriander foliage was 10 % higher than in untreated foliage when stored at 25 °C and 4 °C. This study has established for the first time that coriander foliage is rich in folates, which can be doubled by elicitation and impart 10 % more stability than control during processing and storage.

  11. Performance and Egg Quality of Laying Hens Fed Ration Containing Coriander Seeds (Coriandrum sativum Linn

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    U. Habiyah

    2016-04-01

    Full Text Available The aims of this study were to determine the effect of diets containing different levels of coriander seeds on performance and egg quality of Lohmann Brown laying hens. A total of 96 Lohmann Brown laying hens of 43 weeks of age were randomly allocated into 16 experimental units by assigning a completely randomized design with 4 treatments and 4 replications that kept for 6 weeks. The treatment diets were R0: diet with 0% supplementation of coriander seeds powder (control, R1: diet with supplementation of 1% coriander seeds powder, R2: diet with supplementation of 2% coriander seeds powder, and R3: diet with supplementation of 3% coriander seeds powder. The results showed that supplementation of coriander seeds in diets did not affect egg weight, egg production, and egg mass. Supplementation of coriander seeds 2%-3% significantly (P<0.05 decreased feed consumption and feed conversion ratio. Supplementation of coriander seeds 1%-3% significantly (P<0.05 increased yellowness in yolk color without affecting other quality parameters. It can be concluded that supplementation of coriander seeds at the levels of 2%-3% decreased feed intake, feed conversion ratio, and increased yolk color, however, the supplementation at all levels in diets did not affect egg weight, egg production, and egg mass.

  12. Flavour characterisation and free radical scavenging activity of coriander (Coriandrum sativum L.) foliage.

    Science.gov (United States)

    Priyadarshi, Siddharth; Khanum, Hafeeza; Ravi, Ramasamy; Borse, Babasaheb Baskarrao; Naidu, Madeneni Madhava

    2016-03-01

    The primary objective was to characterize Indian Coriandrum sativum L. foliage (Vulgare alef and Microcarpum DC varieties) and its radical scavenging activity. Foliage of Vulgare alef and Microcarpum DC contained ascorbic acid (1.16 ± 0.35 and 1.22 ± 0.54 mg/g), total carotenoids (1.49 ± 0.38 and 3.08 ± 1.2 mg/g), chlorophyll 'a' (8.23 ± 2.4 and 12.18 ± 2.9 mg/g), chlorophyll 'b' (2.74 ± 0.8 and 4.39 ± 1.3 mg/g) and total chlorophyll (10.97 ± 2.6 and 16.57 ± 3.2 mg/g). The polyphenol content was 26.75 ± 1.85 and 30.00 ± 2.64 mg/g in Vulgare alef and Microcarpum DC, respectively. Ethanol extracts (200 ppm) of alef and Microcarpum DC showed higher radical scavenging activity of 42.05 ± 2.42 % and 62.79 ± 1.36 % when compared with 95 % butylated hydroxyanisole. The principal component analysis results indicated that e-nose can distinguish the volatiles effectively. Quantitative descriptive sensory analysis showed that Microcarpum DC variety is superior to Vulgare alef variety. Nearly 90 % of the flavour compounds present were identified by GC-MS in both varieties. The principal component identified in both the varieties were decanal (7.645 and 7.74 %), decanol  (25.12 and 39.35 %), undecanal (1.20 and 1.75 %), dodecanal (7.07 and 2.61 %), tridecen-1-al  (6.67 and 1.21 %), dodecen-1-ol  (16.68 and 8.05 %), 13-tetradecenal (9.53 and 8.60 %), tetradecanal (5.61 and 4.35 %) and 1-octadecanol (1.25 and 3.67 %).

  13. CORRELATION AND PATH COEFFICIENT ANALYSIS OF PRODUCTIVITY ELEMENTS IN CORIANDER (CORIANDRUM SATIVUM L.

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    Nykolay Hristov Dyulgerov

    2013-12-01

    Full Text Available The aim of the present study was to generate information on interrelationships of some important productivity elements, direct and indirect effects of these characters on fruit yield of 1 plant in coriander. The study was conducted in the Institute of Agriculture - Karnobat, during the period 2006-2008 and included 81 coriander accessions. Phenotypic correlations of fruit weight per plant were highly significant and positive with number of branches per plant, number of umbels per plant, number of fruits per 1 umbel, fruit weight per umbel and 1000-fruits weight. Maximum direct contribution to fruit weight per plant was made by 1000-fruits weight, followed by fruit weight per umbel and number of umbels per plant. Therefore, these traits can be used as selection criteria to increase plant yield in coriander.

  14. Oil and fatty acid accumulation during coriander (Coriandrum sativum L.) fruit ripening under organic cultivation

    Institute of Scientific and Technical Information of China (English)

    Quang-Hung Nguyen; Thierry Talou; Mureil Cerny; Philippe Evon; Othmane Merah

    2015-01-01

    To evaluate the accumulation of oil and fatty acids in coriander during fruit ripening, a field experiment was conducted under organic cultivation conditions in Auch (near Toulouse, southwestern France) during the 2009 cropping season. The percentage and composition of the fatty acids of coriander were determined by gas chromatography. Our results showed that rapid oil accumulation started in early stages (two days after flowering, DAF). Twelve fatty acids were identified. Saturated and polyunsaturated acids were the dominant fatty acids at earlier stages (2–12 DAF), but decreased after this date. After this stage, petroselinic acid increased to its highest amount at 18 DAF. In contrast, palmitic acid followed the opposite trend. Saturated and polyunsaturated fatty acids decreased markedly and monounsaturated fatty acids increased during fruit maturation. It appears that the fruit of coriander may be harvested before full maturity.

  15. Determination of ochratoxin A in coriander (Coriandrum sativum L. by HPCL/fluorescence detection

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    Celeste Matos Lino

    2006-06-01

    Full Text Available An analytical study based on extraction with acetonitrile-water, immunoaffinity column cleanup, and HPLC/fluorescence detection for separation and identification of ochratoxin A in coriander was carried out. Validation of the applied methodology was done through accuracy and precision studies. Homogenized samples of coriander were spiked in triplicate with ochratoxin A at 0.5, 1.0, 2.0, and 5.0 µg/kg levels. Recovery values were in the range of 98% for a fortification level at 0.5 µg/kg to 109.1% at 1.0 µg/kg. Application to coriander samples available in Portuguese markes showed no contamination with ochratoxin A.

  16. Oil and fatty acid accumulation during coriander (Coriandrum sativum L.) fruit ripening under organic cultivation

    Institute of Scientific and Technical Information of China (English)

    Quang-Hung; Nguyen; Thierry; Talou; Mureil; Cerny; Philippe; Evon; Othmane; Merah

    2015-01-01

    To evaluate the accumulation of oil and fatty acids in coriander during fruit ripening, a field experiment was conducted under organic cultivation conditions in Auch(near Toulouse,southwestern France) during the 2009 cropping season. The percentage and composition of the fatty acids of coriander were determined by gas chromatography. Our results showed that rapid oil accumulation started in early stages(two days after flowering, DAF). Twelve fatty acids were identified. Saturated and polyunsaturated acids were the dominant fatty acids at earlier stages(2–12 DAF), but decreased after this date. After this stage,petroselinic acid increased to its highest amount at 18 DAF. In contrast, palmitic acid followed the opposite trend. Saturated and polyunsaturated fatty acids decreased markedly and monounsaturated fatty acids increased during fruit maturation. It appears that the fruit of coriander may be harvested before full maturity.

  17. Oil and fatty acid accumulation during coriander (Coriandrum sativum L. fruit ripening under organic cultivation

    Directory of Open Access Journals (Sweden)

    Quang-Hung Nguyen

    2015-08-01

    Full Text Available To evaluate the accumulation of oil and fatty acids in coriander during fruit ripening, a field experiment was conducted under organic cultivation conditions in Auch (near Toulouse, southwestern France during the 2009 cropping season. The percentage and composition of the fatty acids of coriander were determined by gas chromatography. Our results showed that rapid oil accumulation started in early stages (two days after flowering, DAF. Twelve fatty acids were identified. Saturated and polyunsaturated acids were the dominant fatty acids at earlier stages (2–12 DAF, but decreased after this date. After this stage, petroselinic acid increased to its highest amount at 18 DAF. In contrast, palmitic acid followed the opposite trend. Saturated and polyunsaturated fatty acids decreased markedly and monounsaturated fatty acids increased during fruit maturation. It appears that the fruit of coriander may be harvested before full maturity.

  18. Identification of (E,E)-2,4-undecadienal from coriander (Coriandrum sativum L.) as a highly effective deodorant compound against the offensive odor of porcine large intestine.

    Science.gov (United States)

    Ikeura, Hiromi; Kohara, Kaori; Li, Xin-Xian; Kobayashi, Fumiyuki; Hayata, Yasuyoshi

    2010-10-27

    The leaves of coriander ( Coriandrum sativum L.) exhibited a strong deodorizing effect against porcine internal organs (large intestine). The effective deodorizing compounds of coriander were identified by separating the volatile component of coriander, testing the effectiveness of each fraction against the offensive odor of porcine large intestine, and then identifying the compounds by GC-MS. The volatile component of coriander was first separated into six fractions (A-F) by preparative gas chromatography, and the deodorizing activity of each of these fractions against the offensive odor was measured. Fraction D, which showed the strongest deodorizing effect, was then separated into 12 subfractions by preparative GC. The deodorant activity of each subfraction was evaluated, and the deodorant compounds were identified by GC-MS. It was discovered that (E,E)-2,4-undecadienal was the most effective deodorizing compound. The deodorizing activity of (E,E)-2,4-undecadienal on the porcine large intestine increased as with concentration, reaching almost complete deodorizing ability at 10 ppb.

  19. Impact of blanching on polyphenol stability and antioxidant capacity of innovative coriander (Coriandrum sativum L.) pastes.

    Science.gov (United States)

    Kaiser, Andrea; Kammerer, Dietmar R; Carle, Reinhold

    2013-09-01

    Fresh coriander leaves were steam- and water-blanched at 100 °C and at 90 and 100 °C, respectively, for 1-10 min, and subsequently comminuted to form a paste. Pasty products obtained from coriander fruits were processed after water-blanching applying the same time-temperature regimes. Among the 11 phenolics characterised in leaves by high-performance liquid chromatography coupled to mass spectrometric detection, several caffeic acid derivatives, 5-feruloylquinic and 5-p-coumaroylquinic acids were tentatively identified for the first time. In fruits, 10 phenolics were detected, whereas rutin, a dicaffeic acid derivative and two feruloylquinic and caffeoylquinic acid isomers were newly detected. Upon steam-blanching for 1 min, phenolic contents and antioxidant capacities remained virtually unchanged. In contrast, water-blanching and extended steam-blanching even yielded increased levels compared to the unheated control, whereas short-time water-blanching resulted in higher values than prolonged heat treatment. Thus, short-time water-blanching is recommended as the initial unit in the processing of coriander leaves and fruits into novel pasty products.

  20. Sensory profile of Italian salami with coriander (Coriandrum sativum L. essential oil

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    Cristiane Marangoni

    2011-03-01

    Full Text Available The descriptive terminology and sensory prolife of four samples of Italian salami were determined using a methodology based on the Quantitative Descriptive Analysis (QDA. A sensory panel consensually defined sensory descriptors, their respective reference materials, and the descriptive evaluation ballot. Twelve individuals were selected as judges and properly trained. They used the following criteria: discriminating power, reproducibility, and individual consensus. Twelve descriptors were determined showing similarities and differences among the Italian salami samples. Each descriptor was evaluated using a 10 cm non-structured scale. The data were analyzed by ANOVA, Tukey test, and the Principal Component Analysis (PCA. The salami with coriander essential oil (T3 had lower rancid taste and rancid odor, whereas the control (T1 showed high values of these sensory attributes. Regarding brightness, T4 showed the best result. For the other attributes, T1, T2, T3, and T4 were similar.

  1. Multivariate Analysis for Umbel per plant in Land races of Coriander (Coriandrum sativum L.

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    Hari Shankar Yadava

    2014-10-01

    Full Text Available Twenty five land races from Madhya Pradesh and ten germplasm of coriander were evaluated in four environments to assess umbel per plant using multivariate analysis. Mean sum of squares due to genotypes, environments and GEI were highly significant for umbels per plant. Variation in GEI was mainly due to heterogeneity. . PCA 1 and PCA 2 captures the 99.42% of interaction sum of squares hence, these two principal component axes were the best predictive. The potential environment the potential environments E3 (high fertility, 2009-10 bearing lowest interaction effect while, least potential environments E2 (low fertility, 2008-09 exhibited high PCA scores. The biplot of genotype, environment and IPCA 1 showed three groups. One group exhibited the similar main effects (mean umbels per plant to the grand mean. The second group showed high interaction effect varied in direction while third group bear the low interaction effect. AMMI Stability Values (ASV, ranging from from 7.444 to 31.099 was lowest in RVC 8 followed by RVC 4, RVC 11, RVC 21, RVC 9 and RVC 3 whereas, it was noted maximum in RVC 19 followed by Moroccan, CS 193, Simpo S 33 and G 5363. The genotypes exhibiting low IPCA scores and ASV namely, RVC 8, RVC 4, RVC 11, RVC 21, RVC 19 and RVC 25 showed wider adaptability for umbels per plant while, RVC 19, Moroccan, CS 193, Simpo S 33 and G 5363 exhibiting specific adaptability towards environmental conditions. These genotypes can be utilized in breeding programmes to transfer the adaptability genes for umbel per plant into high yielding genetic back ground in coriander.

  2. Diagnostic of enteric indicators in coriander (Coriandrum sativum and parsley (Petroselinum sativum sold at popular food markets in Quito

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    Dayana Cerón Salgado

    2015-03-01

    Full Text Available (Received: 2015/01/20 - Accepted: 2015/03/25In this research, 108 samples were analyzed (54 of coriander and 54 of parsley. These samples were obtained under a random method in three distribution spots on each of the markets around the north of the city of Quito. Total coliforms (TC, fecal coliforms (FC and Escherichia coli (EC were detected. The average of TC value for coriander was 5.09 log UFC/g and 6.39 log UFC/g for parsley. Values of 607.0 NMP/g for F for the market #1, 597.7NMP/g for the market # 2, and 474.2 NMP/g for the market # 3 were found. The 23.1% of the parsley samples presented positive recounts for Escherichia coli while for the coriander the 14.8% of the samples were positive for this bacterium. The presence of enteric indicators (TC, FC, and EC was independent from the market and the distribution point which indicates a high contamination level and demonstrates the necessity of a microbiologic control on the irrigation system, farming, transport and hygienic conditions of handlers in order to assure the quality of the leafy vegetables, is recommended to set local norms to evaluate the microbiological quality of these foods.

  3. Effect of intercropping carrot (Daucus carota L.) with two aromatic plants, coriander (Coriandrum sativum L.) and summer savory (Satureja hortensis L.), on the population density of select carrot pests

    OpenAIRE

    Jankowska Beata; Wojciechowicz-Żytko Elżbieta

    2016-01-01

    Intercropping, or the use of different aspects of the interaction between organisms in ecosystems, can be classified as an ecological method that limits harmful human interference in the environment, especially the consumption of chemicals. The impact of intercropping carrot with coriander Coriandrum sativum L. and summer savory Satureja hortensis L. on the occurrence of select carrot pests was estimated in the years 2010-2011. Intercropping had a significant effect on the decrease of the num...

  4. Effect of coriander seed (Coriandrum sativum L.) ethanol extract on insulin release from pancreatic beta cells in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Eidi, Maryam; Eidi, Akram; Saeidi, Ali; Molanaei, Saadat; Sadeghipour, Alireza; Bahar, Massih; Bahar, Kamal

    2009-03-01

    Coriander (Coriandrum sativum L.) is grown as a spice crop all over the world. The seeds have been used to treat indigestion, diabetes, rheumatism and pain in the joints. In the present study, an ethanol extract of the seeds was investigated for effects on insulin release from the pancreatic beta cells in streptozotocin-induced diabetic rats. Blood samples were drawn from the retro-orbital sinus before and 1.5, 3 and 5 h after administration of the seed extract. Serum glucose levels were determined by the glucose oxidase method. To determine the insulin releasing activity, after extract treatment the animals were anaesthetized by diethyl ether, the pancreas was excised, fixed in 10% formaldehyde and embedded in paraffin for sectioning. Pancreatic sections of 5 microm were processed for examination of insulin-releasing activity using an immunocytochemistry kit. The results showed that administration of the ethanol extract (200 and 250 mg/kg, i.p.) exhibited a significant reduction in serum glucose. Administration of streptozotocin decreased the number of beta cells with insulin secretory activity in comparison with intact rats, but treatment with the coriander seed extract (200 mg/kg) increased significantly the activity of the beta cells in comparison with the diabetic control rats. The extract decreased serum glucose in streptozotocin-induced diabetic rats and increased insulin release from the beta cells of the pancreas.

  5. Avaliação da atividade antiinflamatória do coentro (Coriandrum sativum L. em roedores Evaluation of the anti-inflammatory activity of coriander (Coriandrum sativum L. in rodents

    Directory of Open Access Journals (Sweden)

    G Zanusso-Junior

    2011-01-01

    Full Text Available Coriandrum sativum L. (Umbelliferae, conhecido popularmente por coentro, é uma planta doméstica cultivada nas diversas partes do mundo, inclusive no Brasil. As folhas e frutos do coentro são utilizados como condimento em culinária e na medicina popular como analgésica, antirreumática, carminativa e colagoga. O objetivo deste estudo foi avaliar o efeito do tratamento com o óleo essencial (OEC e o extrato hidroalcóolico (EHC do coentro em modelos experimentais de inflamação em roedores. A atividade antiinflamatória do coentro foi avaliada por meio dos testes de pleurisia em ratos e formação do edema de orelha em camundongos. A pleurisia foi induzida pela carragenina em animais tratados ou não com EHC. O edema de orelha induzido pela aplicação tópica de óleo de cróton e a atividade da mieloperoxidase foi avaliada em camundongos tratados ou não com OEC ou EHC. No teste da pleurisia o tratamento com EHC promoveu significativa diminuição no edema pleural, mas não sobre a migração leucocitária. Além disso, diferentemente ao observado com o tratamento com OEC, o uso tópico de EHC diminui significativamente o edema de orelha e a migração celular induzidos pela aplicação do óleo de cróton. Os dados indicam que EHC apresenta atividade antiinflamatória quando administrado pelas via oral e tópica, enquanto que OEC não apresenta atividade antiinflamatória tópica.Commonly known as coriander, Coriandrum sativum L. (Umbelliferae is a home plant grown in several parts of the world, including Brazil. Its leaves and fruits have been used as condiment in cooking and in folk medicine as analgesic, antirheumatic, carminative and cholagogue. The aim of this study was to evaluate the effect of essential oil (EO and hydroalcoholic extract (HE from coriander on experimental inflammation models in rodents. Coriander anti-inflammatory activity was evaluated by pleurisy tests in rats and ear edema formation in mice. Pleurisy was induced

  6. Hydroalcoholic seed extract of Coriandrum sativum (Coriander) alleviates lead-induced oxidative stress in different regions of rat brain.

    Science.gov (United States)

    Velaga, Manoj Kumar; Yallapragada, Prabhakara Rao; Williams, Dale; Rajanna, Sharada; Bettaiya, Rajanna

    2014-06-01

    Lead exposure is known to cause apoptotic neurodegeneration and neurobehavioral abnormalities in developing and adult brain by impairing cognition and memory. Coriandrum sativum is an herb belonging to Umbelliferae and is reported to have a protective effect against lead toxicity. In the present investigation, an attempt has been made to evaluate the protective activity of the hydroalcoholic extract of C. sativum seed against lead-induced oxidative stress. Male Wistar strain rats (100-120 g) were divided into four groups: control group: 1,000 mg/L of sodium acetate; exposed group: 1,000 mg/L lead acetate for 4 weeks; C. sativum treated 1 (CST1) group: 250 mg/kg body weight/day for seven consecutive days after 4 weeks of lead exposure; C. sativum treated 2 (CST2) group: 500 mg/kg body weight/day for seven consecutive days after 4 weeks of lead exposure. After the exposure and treatment periods, rats were sacrificed by cervical dislocation, and the whole brain was immediately isolated and separated into four regions: cerebellum, hippocampus, frontal cortex, and brain stem along with the control group. After sacrifice, blood was immediately collected into heparinized vials and stored at 4 °C. In all the tissues, reactive oxygen species (ROS), lipid peroxidation products (LPP), and total protein carbonyl content (TPCC) were estimated following standard protocols. An indicator enzyme for lead toxicity namely delta-amino levulinic acid dehydratase (δ-ALAD) activity was determined in the blood. A significant (psativum resulted in a tissue-specific amelioration of oxidative stress produced by lead.

  7. Coriandrum sativum L. (Coriander essential oil: antifungal activity and mode of action on Candida spp., and molecular targets affected in human whole-genome expression.

    Directory of Open Access Journals (Sweden)

    Irlan de Almeida Freires

    Full Text Available Oral candidiasis is an opportunistic fungal infection of the oral cavity with increasingly worldwide prevalence and incidence rates. Novel specifically-targeted strategies to manage this ailment have been proposed using essential oils (EO known to have antifungal properties. In this study, we aim to investigate the antifungal activity and mode of action of the EO from Coriandrum sativum L. (coriander leaves on Candida spp. In addition, we detected the molecular targets affected in whole-genome expression in human cells. The EO phytochemical profile indicates monoterpenes and sesquiterpenes as major components, which are likely to negatively impact the viability of yeast cells. There seems to be a synergistic activity of the EO chemical compounds as their isolation into fractions led to a decreased antimicrobial effect. C. sativum EO may bind to membrane ergosterol, increasing ionic permeability and causing membrane damage leading to cell death, but it does not act on cell wall biosynthesis-related pathways. This mode of action is illustrated by photomicrographs showing disruption in biofilm integrity caused by the EO at varied concentrations. The EO also inhibited Candida biofilm adherence to a polystyrene substrate at low concentrations, and decreased the proteolytic activity of Candida albicans at minimum inhibitory concentration. Finally, the EO and its selected active fraction had low cytotoxicity on human cells, with putative mechanisms affecting gene expression in pathways involving chemokines and MAP-kinase (proliferation/apoptosis, as well as adhesion proteins. These findings highlight the potential antifungal activity of the EO from C. sativum leaves and suggest avenues for future translational toxicological research.

  8. Coriandrum sativum L. (Coriander) essential oil: antifungal activity and mode of action on Candida spp., and molecular targets affected in human whole-genome expression.

    Science.gov (United States)

    Freires, Irlan de Almeida; Murata, Ramiro Mendonça; Furletti, Vivian Fernandes; Sartoratto, Adilson; Alencar, Severino Matias de; Figueira, Glyn Mara; de Oliveira Rodrigues, Janaina Aparecida; Duarte, Marta Cristina Teixeira; Rosalen, Pedro Luiz

    2014-01-01

    Oral candidiasis is an opportunistic fungal infection of the oral cavity with increasingly worldwide prevalence and incidence rates. Novel specifically-targeted strategies to manage this ailment have been proposed using essential oils (EO) known to have antifungal properties. In this study, we aim to investigate the antifungal activity and mode of action of the EO from Coriandrum sativum L. (coriander) leaves on Candida spp. In addition, we detected the molecular targets affected in whole-genome expression in human cells. The EO phytochemical profile indicates monoterpenes and sesquiterpenes as major components, which are likely to negatively impact the viability of yeast cells. There seems to be a synergistic activity of the EO chemical compounds as their isolation into fractions led to a decreased antimicrobial effect. C. sativum EO may bind to membrane ergosterol, increasing ionic permeability and causing membrane damage leading to cell death, but it does not act on cell wall biosynthesis-related pathways. This mode of action is illustrated by photomicrographs showing disruption in biofilm integrity caused by the EO at varied concentrations. The EO also inhibited Candida biofilm adherence to a polystyrene substrate at low concentrations, and decreased the proteolytic activity of Candida albicans at minimum inhibitory concentration. Finally, the EO and its selected active fraction had low cytotoxicity on human cells, with putative mechanisms affecting gene expression in pathways involving chemokines and MAP-kinase (proliferation/apoptosis), as well as adhesion proteins. These findings highlight the potential antifungal activity of the EO from C. sativum leaves and suggest avenues for future translational toxicological research.

  9. Radical scavenging activity of black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.), and niger (Guizotia abyssinica Cass.) crude seed oils and oil fractions.

    Science.gov (United States)

    Ramadan, Mohamed F; Kroh, Lothar W; Mörsel, Jörg-T

    2003-11-19

    Crude vegetable oils are usually oxidatively more stable than the corresponding refined oils. Tocopherols, phospholipids (PL), phytosterols, and phenols are the most important natural antioxidants in crude oils. Processing of vegetable oils, moreover, could induce the formation of antioxidants. Black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.), and niger (Guizotia abyssinica Cass.) crude seed oils were extracted with n-hexane and the oils were further fractionated into neutral lipids (NL), glycolipids (GL), and PL. Crude oils and their fractions were investigated for their radical scavenging activity (RSA) toward the stable galvinoxyl radical by electron spin resonance (ESR) spectrometry and toward 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical by spectrophotometric method. Coriander seed oil and its fractions exhibited the strongest RSA compared to black cumin and niger seed oils. The data correlated well with the total content of polyunsaturated fatty acids, unsaponifiables, and PL, as well as the initial peroxide values of crude oils. In overall ranking, RSA of oil fractions showed similar patterns wherein the PL exhibited greater activity to scavenge both free radicals followed by GL and NL, respectively. The positive relationship observed between the RSA of crude oils and their color intensity suggests the Maillard reaction products may have contributed to the RSA of seed oils and their polar fractions. The results demonstrate the importance of minor components in crude seed oils on their oxidative stability, which will reflect on their food value and shelf life. As part of the effort to assess the potential of these seed oils, the information is also of importance in processing and utilizing the crude oils and their byproducts.

  10. Accumulation of calcium in the centre of leaves of coriander (Coriandrum sativum L.) is due to an uncoupling of water and ion transport.

    Science.gov (United States)

    Kerton, Matt; Newbury, H John; Hand, David; Pritchard, Jeremy

    2009-01-01

    The aim of this study is to understand the parameters regulating calcium ion distribution in leaves. Accumulation of ions in leaf tissue is in part dependent on import from the xylem. This import via the transpiration stream is more important for ions such as calcium that are xylem but not phloem mobile and cannot therefore be retranslocated. Accumulation of calcium was measured on bulk coriander leaf tissue (Coriandrum sativum L. cv. Lemon) using ion chromatography and calcium uptake was visualized using phosphor-images of (45)Ca(2+). Leaves of plants grown in hydroponics had elevated calcium in the centre of the leaf compared with the leaf margin, while K(+) was distributed homogeneously over the leaf. This calcium was shown to be localised to the mesophyll vacuoles using EDAX. Stomatal density and evapotranspiration (water loss per unit area of leaf) were equal at inner and outer sections of the leaf. Unequal ion distribution but uniformity of water loss suggested that there was a difference in the extent of uncoupling of calcium and water transport between the inner and outer leaf. Since isolated tissue from the inner and outer leaf were able to accumulate similar amounts of calcium, it is proposed that the spatial variation of leaf calcium concentration is due to differential ion delivery to the two regions rather than tissue/cell-specific differences in ion uptake capacity. There was a positive correlation between whole leaf calcium concentration and the difference in calcium concentration between inner and outer leaf tissue. Exposing the plants to increased humidity reduced transpiration and calcium delivery to the leaf and abolished this spatial variation of calcium concentration. Mechanisms of calcium delivery to leaves are discussed. An understanding of calcium delivery and distribution within coriander will inform strategies to reduce the incidence of calcium-related syndromes such as tip-burn and provides a robust model for the transport of ions and

  11. [Prevalence of Listeria monocytogenes in fresh tomatoes (Lycopersicum esculentum) and coriander (Coriandrum sativum) in three markets of Valencia, Venezuela].

    Science.gov (United States)

    Ramírez Mérida, Luis Guillermo; Morón de Salim, Alba; Alfieri Graterol, Ana Yudith; Gamboa, Orlando

    2009-09-01

    The incidence of L. monocytogenes in tomatoes and coriander obtained from three different markets, during eight weeks were determined. 192 samples were evaluated: 96 of tomatoes, and 96 of coriander. The isolation of L. monocytogenes was performed using COVENIN 3718:2001. The data were analyzed by SPSS version 12.0. Kolmogorov Smirnov, Mann Whitney U, Kruskal Wallis U test; Spearman's correlation were applied, and p<0.05 significance level was aplied. It was not found significant differences between the medias values and standard deviations of Most Probable Number (MPN) of Listeria spp to tomatoes and coriander during the eight weeks of recollection in the markets; neither between the distributions of MPN of tomatoes and coriander from the markets (Chi2=5,233 p<0,073; Chi2=1,624 p<0,444 respectively) neither the samples per weeks (Chi2=6,547 p<0,477; Chi2=2,667 p<0,914 respectively). In the number of MPN between tomatoes and coriander both distributions were significant different according to test U Mann Whitney U=3040,5 (Z=-4,216 p<0,0001). It was found statistical significance (p<0,001) between the number of MPN of tomatoes and coriander. The presence of Listeria spp in tomatoe was 41,66% (25,0% L. monocytogenes and 16,7% L. ivanovii); in coriander 77,08% (36,5% L. monocytogenes, 33,3% L. ivanovii and 7,3% L. seelige). We concluded that the high level of L. monocytogenes in tomatoes and coriander is independent of the markets store; we see the necessity of a microbiological control on the irrigation system, collection and distribution to ensure the quality of the product.

  12. Potential of coriander (Coriandrum sativum) oil as a natural antimicrobial compound in controlling Campylobacter jejuni in raw meat.

    Science.gov (United States)

    Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat

    2010-01-01

    Twelve essential oils were tested in vitro for antimicrobial activities against several strains of Campylobacter jejuni, a pathogen causing food-borne diseases worldwide. Using disk diffusion and minimal inhibitory concentration determination assays, we noted that coriander oil exhibited the strongest antimicrobial activity against all tested strains. The oil had a bactericidal effect on the target bacteria. In evaluating the antimicrobial potency of coriander oil against C. jejuni on beef and chicken meat at 4 degrees C and 32 degrees C, it was found that the oil reduced the bacterial cell load in a dose-dependent manner. The type of meat and temperature did not influence the antimicrobial activity of the oil. This study indicates the potential of coriander oil to serve as a natural antimicrobial compound against C. jejuni in food.

  13. Effects of Different Fertilizer Managements on Quantitative and Qualitative Characteristics of Coriander (Coriandrum sativum L. as a Medicinal Plant

    Directory of Open Access Journals (Sweden)

    R Bigonah

    2015-04-01

    Full Text Available In order to study the effect of mycorrhiza and vermin compost on quantitative characteristics and essential oil content of seeds of Coriander, a field experiment was conducted during growing season of 2010 at Agriculture Research Station, College of Agriculture, Ferdowsi University of Mashhad, Iran. A randomized complete block design with three replications was used. Treatments included: 1 vermin compost, 2 mycorrhiza, 3 phosphorus, 4 mycorrhiza + vermicompost, 5 mycorrhiza + phosphorus, 6 vermin compost + phosphorus, 7 control (without fertilizer. Each plot was divided to two parts, in order to study green biological yield and seed production. Green biological yield harvested in two times at 5% flowering stage and harvesting seed yield was done when majority of plants got yellow. The result showed that vermin compost and control treatments had the highest and the lowest seed yield and plant height respectively. Mycorrhiza and mycorrhiza + phosphorus treatments had the highest number of umbel per plant, and the lowest number of umbel was shown in control treatment. Control and vermin compost treatments had the highest and lowest essential oil contents of seeds, respectively. In addition vermin compost and mycorrhiza treatments in the first and second cuts had the highest of green biological yield and dry weight of leaf per plant, respectively. Control treatment had the lowest biological yield and dry weight of leaf per plant, in both cuts.

  14. Chemopreventive role of Coriandrum sativum against gentamicin-induced renal histopathological damage in rats.

    Science.gov (United States)

    Lakhera, Abhijeet; Ganeshpurkar, Aditya; Bansal, Divya; Dubey, Nazneen

    2015-06-01

    Drug induced nephrotoxicity is one of the most common causes of renal failure. Gentamicin belongs to aminoglycosides, which elicit nephrotoxic potential. Natural antioxidants from plants demonstrate a number of biotherapeutic activities. Coriander is an important medicinal plant known for its hepatoprotective, diuretic, carminative, digestive and antihelminthic potential. This study was designed to investigate whether the extract of Coriandrum sativum ameliorates the nephrotoxicity induced by gentamicin in rats. Dried coriander powder was coarsely grinded and subjected to defatting by petroleum ether and further with ethyl acetate. The extract was filtered and subjected to phytochemical and phytoanalytical studies. Acute toxicity in Wistar rats was determined by the OECD Guideline (423). Animals were divided into four groups. The first group served as positive control, while the second group was toxic control (gentamicin treated). The third and fourth group were treated with the extract (200 and 400 mg/kg gentamicin). After 8 days, the animals were sacrificed and biochemical and histopathological studies were carried out. Phytochemical screening of the extract demonstrated Coriandrum sativum to be rich in flavonoids, polyphenolics and alkaloids. Results of acute toxicity suggested the use of 200 mg/kg and 400 mg/kg for Coriandrum sativum in the study. Coriandrum sativum extract at the dose of 400 mg/kg significantly (pCoriandrum sativum extract ameliorated renal histological lesions. It is concluded that Coriandrum sativum is a potential source of nephroprotective phytochemical activity, with flavonoids and polyphenols as the major components.

  15. Effect of intercropping carrot (Daucus carota L. with two aromatic plants, coriander (Coriandrum sativum L. and summer savory (Satureja hortensis L., on the population density of select carrot pests

    Directory of Open Access Journals (Sweden)

    Jankowska Beata

    2016-06-01

    Full Text Available Intercropping, or the use of different aspects of the interaction between organisms in ecosystems, can be classified as an ecological method that limits harmful human interference in the environment, especially the consumption of chemicals. The impact of intercropping carrot with coriander Coriandrum sativum L. and summer savory Satureja hortensis L. on the occurrence of select carrot pests was estimated in the years 2010-2011. Intercropping had a significant effect on the decrease of the number of roots damaged by carrot rust fly Psila rosae. During harvest, the least number of damaged roots was observed in combination with summer savory S. hortensis L. The number of carrot psyllid Trioza viridula Zett. and aphids on carrot leaves and roots damaged by nematodes was significantly lower on plots where carrot was intercropped. Intercropping both herbs had a positive influence on the number of beneficial insects. In all of the years of the study, the highest number of Coccinellidae and Syrphidae were observed on plots where carrot was intercropped with coriander.

  16. Effects of black pepper (piper nigrum), turmeric powder (curcuma longa) and coriander seeds (coriandrum sativum) and their combinations as feed additives on growth performance, carcass traits, some blood parameters and humoral immune response of broiler chickens.

    Science.gov (United States)

    Abou-Elkhair, R; Ahmed, H A; Selim, S

    2014-06-01

    Different herbs and spices have been used as feed additives for various purposes in poultry production. This study was conducted to assess the effect of feed supplemented with black pepper (Piper nigrum), turmeric powder (Curcuma longa), coriander seeds (Coriandrum sativum) and their combinations on the performance of broilers. A total of 210 (Cobb) one-d-old chicks were divided into seven groups of 30 birds each. The treatments were: a control group received no supplement, 0.5% black pepper (T1), 0.5% turmeric powder (T2), 2% coriander seeds (T3), a mixture of 0.5% black pepper and 0.5% turmeric powder (T4), a mixture of 0.5% black pepper and 2% coriander seed (T5), and a mixture of 0.5% black pepper, 0.5% turmeric powder and 2% coriander seeds (T6). Higher significant values of body weight gain during the whole period of 5 weeks (p<0.001) were observed in broilers on T1, T3, T5, and T6 compared to control. Dietary supplements with T1, T2, T3, and T6 improved the cumulative G:F of broilers during the whole period of 5 weeks (p<0.001) compared with control. The dressing percentage and edible giblets were not influenced by dietary supplements, while higher values of relative weight of the liver (p<0.05) were obtained in T5 and T6 compared to control. The addition of feed supplements in T5 and T6 significantly increased serum total protein and decreased serum glucose, triglycerides and alkaline phosphatase concentrations compared with the control group (p<0.05). Broilers on T6 showed significant decrease in the serum glutamate pyruvate transaminase concentration (p<0.05) compared to control. The broilers having T5 and T6 supplemented feed had relatively greater antibody titre (p<0.001) at 35 d of age than control. It is concluded that dietary supplements with black pepper or coriander seeds or their combinations enhanced the performance and health status of broiler chickens.

  17. Effects of dietary mixture of garlic (Allium sativum), coriander (Coriandrum sativum) and probiotics on immune responses and caecal counts in young laying hens.

    Science.gov (United States)

    Lee, J S; Kim, M J; Park, S H; Lee, S B; Wang, T; Jung, U S; Im, J; Kim, E J; Lee, K W; Lee, H G

    2016-09-28

    This study was conducted to evaluate the effects of a combined mixture of phytogenic extracts (garlic and coriander) and probiotics on growth performance and immune responses in laying hens based on the results of in vitro studies to screen for immunomodulatory potency of each ingredient. Several parameters of immunomodulatory potency were estimated using lamina propria leucocytes (LPLs) isolated from rat intestinal mucosa tissue. Results show that the combined mixture enhanced LPLs proliferation, increased LPL-mediated cytotoxicity against YAC-1 tumour cells, and decreased lipopolysaccharide (LPS)-induced cytokine production including tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in LPLs. For in vivo study, laying hens (n = 50/each diet group) were fed with control diet, a diet containing antibiotics (0.01% per kg feed) or the combined mixture (0.02% per kg feed) for 21 days. The dietary combined mixture improved egg production (p < 0.05) but not growth performance and carcass traits. Interestingly, the patterns of suppressing plasma IFN-γ productions during inflammation by LPS injection and decreasing caecal E. coli counts in the combined mixture group were comparable to those in the antibiotics group. Taken together, our results suggested that the 0.02% of combined mixture of phytogenic extracts and probiotics as ingredients has potential immunomodulatory effects in laying hens.

  18. Influence of seed priming on emergence and growth of coriander (Coriandrum sativum L.) seedlings grown under salt stress / Vpliv pretretiranja semen koriandra (Coriandrum sativum L.) s solnimi raztopinami na vznik in rast v razmerah solnega stresa

    National Research Council Canada - National Science Library

    Elouaer Mohamed Aymen; Hannachi Cherif

    2013-01-01

    .... For this reason, an experiment was conducted to study the effect of seed priming with NaCl and CaCl on growth and yield responses of Tunisian coriander cultivar exposed to five levels of salinity (0, 2, 4, 6, 8 g l...

  19. Chemopreventive role of Coriandrum sativum against gentamicin-induced renal histopathological damage in rats

    Directory of Open Access Journals (Sweden)

    Lakhera Abhijeet

    2015-06-01

    Full Text Available Drug induced nephrotoxicity is one of the most common causes of renal failure. Gentamicin belongs to aminoglycosides, which elicit nephrotoxic potential. Natural antioxidants from plants demonstrate a number of biotherapeutic activities. Coriander is an important medicinal plant known for its hepatoprotective, diuretic, carminative, digestive and antihelminthic potential. This study was designed to investigate whether the extract of Coriandrum sativum ameliorates the nephrotoxicity induced by gentamicin in rats. Dried coriander powder was coarsely grinded and subjected to defatting by petroleum ether and further with ethyl acetate. The extract was filtered and subjected to phytochemical and phytoanalytical studies.

  20. Effects of Different Levels of Coriander (Coriandrum sativum Seed Powder and Extract on Serum Biochemical Parameters, Microbiota, and Immunity in Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Hesam Hosseinzadeh

    2014-01-01

    Full Text Available The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28 d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.

  1. Effects of different levels of coriander (Coriandrum sativum) seed powder and extract on serum biochemical parameters, microbiota, and immunity in broiler chicks.

    Science.gov (United States)

    Hosseinzadeh, Hesam; Alaw Qotbi, Ali Ahmad; Seidavi, Alireza; Norris, David; Brown, David

    2014-01-01

    The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28 d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.

  2. Spectral quality of photo-selective nets improves phytochemicals and aroma volatiles in coriander leaves (Coriandrum sativum L.) after postharvest storage.

    Science.gov (United States)

    Buthelezi, Millicent N Duduzile; Soundy, Puffy; Jifon, John; Sivakumar, Dharini

    2016-08-01

    The influence of spectral light on leaf quality and phytochemical contents and composition of aroma compounds in coriander leaves grown for fresh use under photo-selective nets; pearl net [40% shading; and 3.88 blue/red ratio; 0.21 red/far red ratio; photosynthetic radiation (PAR) 233.24 (μmolm(-2)s(-1))] and red net [40% shading and 0.57 blue/red ratio; 0.85 red/far red ratio; 221.67 (μmolm(-2)s(-1))] were compared with commercially used black nets [25% shading; 3.32 blue/red ratio 0.96 red/far red ratio; 365.26 (μmolm(-2)s(-1))] at harvest and after 14days of storage. Black nets improved total phenols, flavonoid (quercetin) content, ascorbic acid content, and total antioxidant activity in coriander leaves at harvest. The characteristic leaf aroma compound decanal was higher in leaves from the plants under the red nets at harvest. However, coriander leaves from plants produced under red nets retained higher total phenols, flavonoids (quercetin) and antioxidant scavenging activity 14days after postharvest storage (0°C, 10days, 95% RH and retailers' shelf at 15°C for 4days, 75% RH). But production under the pearl nets improved marketable yield reduced weight loss and retained overall quality, ascorbic acid content and aroma volatile compounds in fresh coriander leaves after postharvest storage. Pearl nets thus have the potential as a pre-harvest tool to enhance the moderate retention of phytochemicals and saleable weight for fresh coriander leaves during postharvest storage.

  3. Essential oil compositions of different accessions of Coriandrum sativum L. from Iran.

    Science.gov (United States)

    Nejad Ebrahimi, Samad; Hadian, Javad; Ranjbar, Hamid

    2010-09-01

    Coriander (Coriandrum sativum L.) has been cultivated for a many years in different parts of Iran. The chemical profiles of different accessions were analysed by means of GC-MS. The essential oil content of the dried seeds varied from 0.1% to 0.36%. Thirty-four different compounds were identified in the essential oil of all accessions. Linalool (40.9-79.9%), neryl acetate (2.3-14.2%), gamma-terpinene (0.1-13.6%) and alpha-pinene (1.2-7.1%) were identified as main components in the oil of the coriander accessions. Almost all of the studied accessions contained more that 60% linalool, showing the high quality of coriander seeds produced in Iran and the suitability of the accessions as initial genetic materials for the breeding of homogenous and talented Coriander cultivars.

  4. Scientific basis of use of fruits Coriandrum sativum L. In food technologies

    Directory of Open Access Journals (Sweden)

    Natalia Frolova

    2016-10-01

    Full Text Available Today in the world recognized the need for environmentally friendly products for a healthy food and quality life. Products with natural ingredients, including flavoring become very popular. Coriander is one of herbs that functions as both, spice as well as herbal medicine. Coriandrum sativum L. is a major aromatic crop in Ukraine. The plants of Coriandrum sativum contain the essential oils and other compounds in the seeds and leaves and have an important role as flavorings. The main objective was to investigate possibility effective utilization of coriander essential oil in national economy of Ukraine. It was necessary to study the chemical compounds of coriander fruits by instrumental analysis and odor by sensory analysis with following creating new aroma compositions. Search had been carried out throughout 2009 - 2014 years. The aerial parts of aromatic plants were harvested at the plots of National Botanical Garden of National Academy of the Sciences of Ukraine. Essential oil was obtained by hydro distillation procedure in National University of food technology. Main and specific components of essential oils from seeds coriander were characterized. Qualitative structure of essential oils was determined by the gas-liquid chromatography method on the chromatograph Agilent Technologies 6890 with mass-spectrometric detector 5973. The run of components was done using Device of Fractional Distillation. Linalool, limonene, geranyl acetate, d-camphor, myrcene and geraniol were found as the major components. In the composition of essential oils each component has its own flavor, the combination of which determines the flavor of the oil. We investigated the possibility of target separation of essential oils of coriander fruits into fractions of different flavor. The article presents the results of research sequential processing fruits Coriandrum sativum to obtain a series of natural flavors. Principles and laws of the vacuum distillation were used for

  5. Effect of priming on growth, biochemical parameters and mineral composition of different cultivars of coriander (Coriandrum sativum L. under salt stress

    Directory of Open Access Journals (Sweden)

    Ben Fredj Meriem

    2014-08-01

    Full Text Available At Higher Institute of Agriculture of Chott Mariem, Sousse, Tunisia, this study was conducted to evaluate the interactive effect of salinity and seed priming on coriander. The experiment was carried out in completely randomized design with three replications consisting of four coriander genotypes (Tunisian cv, Algerian cv, Syrian cv and Egyptian cv at two seed conditions (seed priming with 4 g/l NaCl for 12h or no seed priming. Results revealed that seed priming and salinity had significantly (p≤0.05 affected all the parameters under study. On the first hand, salinity stress had adversely affected growth, chlorophyll content, mineral composition (K+ and Ca2+ of coriander in all genotypes. Also, it activated Na+ accumulation and synthesis of proline, soluble sugars and proteins. However, seed priming with NaCl had diminished the negative impact of salt stress in all cultivars and primed plants showed better response to salinity compared to unprimed plants. Maximum values were recorded in tolerant cultivar which is Tunisian one whereas minimum values were noted in sensitive cultivar (Algerian cv.

  6. Microbiological analysis of pre-packed sweet basil (Ocimum basilicum) and coriander (Coriandrum sativum) leaves for the presence of Salmonella spp. and Shiga toxin-producing E. coli.

    Science.gov (United States)

    Delbeke, Stefanie; Ceuppens, Siele; Jacxsens, Liesbeth; Uyttendaele, Mieke

    2015-09-02

    Enteric pathogens, such as Salmonella spp. and pathogenic Escherichia coli, have been detected and associated with food borne outbreaks from (imported) fresh leafy herbs. Screening on imported herbs from South East Asian countries has been described. However, limited information on prevalence of these pathogens is available from other sourcing regions. Therefore, fresh pre-packed basil and coriander leaves from a Belgian trading company were investigated for the presence of Salmonella spp., Shiga toxin-producing E. coli (STEC), generic E. coli and coliforms. In total 592 samples were collected originating from Belgium, Israel and Cyprus during 2013-2014. Multiplex PCR followed by further culture confirmation was used for the detection of Salmonella spp. and STEC, whereas the Petrifilm Select E. coli and VRBL-agar were used, respectively, for the enumeration of E. coli and coliforms. Salmonella was detected in 10 out of 592 samples (25g) (1.7%; 5 from basil and 5 from coriander), of which two samples were sourced from Israel and eight from Cyprus. The presence of STEC was suspected in 11 out of 592 samples (25g) (1.9%; 3 basil and 8 coriander), due to the detection of stx and eae genes, of which one sample originated from Belgium, four from Israel and six from Cyprus. No STEC was isolated by culture techniques, but in three samples a serotype (O26, O103 or O111) with its most likely associated eae-variant (β or θ) was detected by PCR. Generic E. coli was enumerated in 108 out of 592 samples, whereby 55, 32 and 13 samples respectively between 10-100, 100-1000 and 1000-10,000cfu/g and 8 samples exceeding 10,000cfu/g. Coliforms were enumerated in all herb samples at variable levels ranging from 1.6 to 7.5logcfu/g. Further statistics indicate that the E. coli class (categorized by level) was significantly correlated with the presence of Salmonella (pE. coli class is a better indicator for the presence of enteric pathogens than coliforms on fresh herbs, but the

  7. New report of Phoma glomerata on Coriandrum sativum L.

    Science.gov (United States)

    Rodeva, R; Carrieri, R; Stoyanova, Z; Dacheva, S; Lahoz, E; Fanigliulo, A; Crescenzi, A

    2013-01-01

    In 2011, coriander plants (Coriandrum sativum L.), showing symptoms of greyish to hell brown lesions with dark brown border between discoloured and asymptomatic tissues, were observed in Bulgaria. Pycnidia with small unicellular conidia appeared in the pale centre of the lesions. In this work, the identification of the causal agent of this disease was made applying Koch's postulates. Moreover, for a rapid and unambiguous identification of the fungal species, the internal transcribed spacers and the 5.8 rDNA gene (ITS1-5.8-ITS2) were amplified by PCR from DNA extracted from the isolated colonies. The amplicon was sequenced and analyzed using BLASTn, and showed a homology of 100% with a corresponding sequence of Phoma glomerata (accession number DQ093699). The fungus isolated, after the morphological and molecular characterization, was ascribed to the species Phoma glomerata (Corda) Wollenweber and Hochapfel. This is the first report of P. glomerata as agent of stem rot of coriander in Bulgaria and elsewhere.

  8. CORIANDRUM SATIVUM- REVIEW OF ADVANCES IN PHYTOPHARMACOLOGY

    Directory of Open Access Journals (Sweden)

    Abidhusen H. Momin*, Sawapnil S. Acharya and Amit V. Gajjar

    2012-05-01

    Full Text Available Coriandrum Sativum family Umbelliferae is highly reputed ayurvedic medicinal tree commonly known as the Dhanya. It is a glabrous, aromatic, herbaceous annual plant, small sized tree growing throughout India, Italy, Netherlands, Central and Eastern Europe, China and Bangladesh. Essential oil, flavonoids, fatty acids, and sterols have been isolated from different parts of C. sativum. The different parts of this plant contain monoterpenes, α-pinene, limpnene, γ-terpinene, p-cymene, borneol, citronellol, camphor, geraniol, coriandrin, dihydrocoriandrin, coriandrons A-E, flavonoids and essential oils. Various parts of this plant such as seed, leaves, flower and fruit, possess antioxidant activity, anti-diabetic activity, anti-mutagenic activity, anti-helmintic activity, sedative-hypnotic activity, anticonvulsant activity , diuretic activity, cholesterol lowering activity, protective role against lead toxicity, antifungal activity, anti-feeding activity, anticancer activity, anxiolytic activity, hepatoprotective activity, anti-protozoal activity, anti-ulcer activity, post-coital anti-fertility activity, heavy metal detoxification. Various phytopharmacological evaluations have been reported in this literature for the important potential of the Coriandrum sativum.

  9. Effect of adding essential oils of coriander (Coriandrum sativum L.) and hyssop (Hyssopus officinalis L.) on the shelf life of ground beef.

    Science.gov (United States)

    Michalczyk, Magdalena; Macura, Ryszard; Tesarowicz, Iwona; Banaś, Joanna

    2012-03-01

    This study examined the effect of adding essential oils of hyssop and coriander at the highest concentration (0.02% v/w) sensorially acceptable to a panel of assessors on the microbiological and biochemical characteristics of stored ground beef. Vacuum-packed meat was stored at 0.5±0.5°C and 6±1°C for 15days. The greatest beneficial effect of both additives was in inhibiting the development of undesirable sensory changes (extending acceptability by up to 3days) and the growth of Enterobacteriaceae (by up to approximately 1-2 log cycles compared with the controls). The effect on lactic acid bacteria, total viable bacterial count and other groups of microorganisms investigated was minor (up to 1 log cycle) and similar for both oils. Neither did these additives significantly affect amino nitrogen levels, protease activity, the proportions of meat pigments, protein electropherograms and pH levels. This indicates the limited effect of these essential oils in the concentrations applied on preserving vacuum-packed minced beef.

  10. A comparison of yield-related traits of Coriandrum sativum var. microcarpum DC. and Coriandrum sativum var. sativum

    OpenAIRE

    Nikolay Dyulgerov; Boryana Dyulgerova

    2014-01-01

    In this investigation we studied how yield-related traits of large-fruited (var. sativum) and small-fruited (var. microcarpum DC.) coriander differ at the Southeastern Bulgaria climatic conditions during 2010-2012. For this purpose, 20 genotypes from var. microcarpum and 20 genotypes from var. sativum were tested using a randomized complete block design with three replications at the Institute of Agriculture – Karnobat. Plant height, number of primary branches per plant, number of umbels per ...

  11. Antibacterial activities of Emblica officinalis and Coriandrum sativum against Gram negative urinary pathogens.

    Science.gov (United States)

    Saeed, Sabahat; Tariq, Perween

    2007-01-01

    Present investigation is focused on antibacterial potential of aqueous infusions and aqueous decoctions of Emblica officinalis (amla) and Coriandrum sativum (coriander) against 345 bacterial isolates belonging to 6 different genera of Gram negative bacterial population isolated from urine specimens by employing well diffusion technique. Aqueous infusion and decoction of Emblica officinalis exhibited potent antibacterial activity against Escherichia coli (270), Klebsiella pneumoniae (51), K. ozaenae (3), Proteus mirabilis (5), Pseudomonas aeruginosa (10), Salmonella typhi (1), S. paratyphi A (2), S. paratyphi B (1) and Serratia marcescens (2) but did not show any antibacterial activity against Gram negative urinary pathogens.

  12. Variations in the essential oil composition from different parts of Coriandrum sativum L. cultivated in Tunisia.

    Science.gov (United States)

    Msaada, Kamel; Hosni, Karim; Taarit, Mouna Ben; Chahed, Thouraya; Marzouk, Brahim

    2007-03-01

    Essential oils obtained by hydrodistillation from different organs (flowers, leaves, stems and roots) of Tunisian coriander (Coriandrum sativum L.) was analyzed. GC and GC-MS analysis enabled us to identify 64 compounds and revealed great qualitative and quantitative differences between the analyzed parts. In all organs, the main compound was (E)-2-dodecenal, followed by (E)-2-tridecenal, gamma-cadinene, (Z)-myroxide, neryl acetate and eugenol. Multivariate analysis (PCA) revealed a high similarity in the essential oils composition between upper leaves and flowers in one hand and basal leaves, roots and stems on the other hand. Further, it has been reported an organ-dependant distribution of essential oil compounds.

  13. Sedative effect of central administration of Coriandrum sativum essential oil and its major component linalool in neonatal chicks.

    Science.gov (United States)

    Gastón, María Soledad; Cid, Mariana Paula; Vázquez, Ana María; Decarlini, María Florencia; Demmel, Gabriela I; Rossi, Laura I; Aimar, Mario Leandro; Salvatierra, Nancy Alicia

    2016-10-01

    Context Coriandrum sativum L. (Apiaceae) (coriander) is an herb grown throughout the world as a culinary, medicinal or essential crop. In traditional medicine, it is used for the relief of anxiety and insomnia. Systemic hydro-alcoholic and aqueous extract from aerial parts and seeds had anxiolytic and sedative action in rodents, but little is known about its central effect in chicks. Objective To study the effects of intracerebroventricular administration of essential oil from coriander seeds and its major component linalool on locomotor activity and emotionality of neonatal chicks. Materials and methods The chemical composition of coriander essential oil was determined by a gas-chromatographic analysis (> 80% linalool). Behavioural effects of central administration of coriander oil and linalool (both at doses of 0.86, 8.6 and 86 μg/chick) versus saline and a sedative diazepam dose (17.5 μg/chick, standard drug) in an open field test for 10 min were observed. Results Doses of 8.6 and 86 μg from coriander oil and linalool significantly decreased (p Coriandrum sativum seeds induced a sedative effect at 8.6 and 86 μg doses. This effect may be due to monoterpene linalool, which also induced a similar sedative effect, and, therefore, could be considered as a potential therapeutic agent similar to diazepam.

  14. Studies on genetic divergence among Indian varieties of a spice herb, Coriandrum sativum.

    Science.gov (United States)

    Singh, S K; Kakani, R K; Meena, R S; Pancholy, Anjly; Pathak, Rakesh; Raturi, Aparna

    2012-07-01

    Coriander (Coriandrum sativum L.) is an annual spice herb that belongs to umbel family Apiaceae with diversified uses. We investigated the extent of variability among 22 Indian varieties of coriander using phenotypic and genetic markers. Multilocus genotyping by nine RAPD primers detected an average of intraspecific variations amounting to 66.18% polymorphism in banding patterns. Analysis of molecular variance indicated that a greater proportion of total genetic variation exists within population (98%) rather than among populations (2%). Higher values of Nei's gene diversity (h) and Shannon Information Index (i) and genetic distance analysis validate wider genetic diversity among Indian coriander varieties. Besides total internal transcribed spacer (ITS) length variations and single nucleotide polymorphisms, insertions/deletions (INDELS) were detected at seven sites in ITS-1 region. Multiple sequence alignment of 12 sequenced varieties revealed cent per cent identities of 5.8S gene region (162 bp) that validates its conserved nature. Multiple sequence alignment of ITS-1 region may be of phylogenetic significance in distinguishing and cataloguing of coriander germplasm. The representative sequences of each subgroup and all distinct varieties of RAPD clusters have been submitted to NCBI database and assigned Gen Accession numbers HQ 377194-377205. The measures of relative genetic distances among the varieties of coriander did not completely correlate the geographical places of their development. Eventually, the knowledge of their genetic relationships and DNA bar coding will be of significance.

  15. VARIABILITY IN ETHIOPIAN CORIANDER ACCESSIONS FOR ...

    African Journals Online (AJOL)

    Administrator

    2010-05-25

    May 25, 2010 ... Coriander (Coriandrum sativum L.) is an annual spice herb that belongs to the family of Umbelliferae/Apiaceae. Although Ethiopia is ... (Holland et al., 1991); iron, manganese, ..... John Willy and Sons., Inc., New York. 663pp.

  16. Quality control and in vitro antioxidant potential of Coriandrum sativum Linn.

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    Mhaveer Singh

    2015-01-01

    Full Text Available Background: Coriandrum sativum Linn., commonly known as coriander, is a well-known spice and drug in India. It has various health-related benefits and used in various Unani formulations. In this present study, quality assessment of coriander fruits was carried out by studying anatomical characters, physicochemical tests, and chemoprofiling using high performance thin layer chromatography (HPTLC and gas chromatography-mass spectroscopy (GC-MS along with in vitro antioxidant potential. Materials and Methods: Standardization was carried out as per the pharmacopeial guidelines. Estimation of heavy metals, pesticides, and aflatoxins was carried out to ascertain the presence of any contaminant in the sample. Chemoprofiling was achieved by thin layer chromatography (TLC by optimizing the mobile phase for different extracts. The most of the pharmacological activities of coriander are based on volatile oil constituents. Hence, GC-MS profiling was also carried out using hexane-soluble fraction of hydro-alcoholic extract. The total phenolic contents and in vitro antioxidant efficacy were determined using previously established methods. Results: The quality control and anatomical studies were very valuable for the identification whereas good antioxidant potential was observed when compared to ascorbic acid. The drug was found free of contaminant when analyzed for pesticides and aflatoxins whereas heavy metals were found under reported limits. Conclusion: The work embodied in this present research can be utilized for the identification and the quality control of the coriander fruit.

  17. Estolides from Coriander Fatty Acids

    Science.gov (United States)

    Coriander (Coriandrum sativum L.) is a new crop that is currently being investigated for cultivation in Central Illinois by USDA-NCAUR. Coriander is an annual herb belonging to the Apiaceae (Umbelliferae) family and indigenous to the Mediterranean basin areas and the Near East. This plant is widel...

  18. Mutagenicity and safety evaluation of water extract of Coriander sativum leaves.

    Science.gov (United States)

    Reyes, Mariana Ramírez; Reyes-Esparza, Jorge; Angeles, Oscar Torres; Rodríguez-Fragoso, Lourdes

    2010-01-01

    Coriander has been used as a spice and medicinal plant for centuries. Several studies have described its biological properties and some reports have indicated its pharmacological actions in some human pathology. However, data on its toxicity and metabolism are limited or null, and no research has been conducted with mammalian cells. The purpose of this study was to evaluate the mutagenicity and safety of Coriandrum sativum extract. The mutagenic effects of C. sativum extract were evaluated by Ames test. Mutagenicity was present when the C. sativum extract was used in high concentrations in both tested strains (Salmonella typhimurium TA97 and TA102). Our research showed that C. sativum extract reduced the cell survival of human cell lines (WRL-68 and 293Q cells) by inducing apoptosis and necrosis in the cases where extract concentration was the highest. The C. sativum extract altered the cell cycle; it increased the G1 phase of hepatic cells and reduced the G2+M phase in both cell lines in a dose-response manner. These results showed correlation with a reduction in the mitotic index. The extract also induced severe malformations during embryonic development. Exposure of chicken embryos to the C. sativum extract resulted in a dose-dependent increase of anomalies. Present results show that C. sativum extract reduced the axial skeleton and affected the neural tube, the somites, the cardiovascular structures, and the eye. According to the present results, the C. sativum aqueous extract cannot be considered safe. These results indicate that some significant adverse effects of C. sativum extract could be observed in vivo.

  19. Transcriptome profiling, and cloning and characterization of the main monoterpene synthases of Coriandrum sativum L.

    Science.gov (United States)

    Galata, Mariana; Sarker, Lukman S; Mahmoud, Soheil S

    2014-06-01

    Terpenoids are a large and diverse class of specialized metabolites that are essential for the growth and development of plants, and have tremendous industrial applications. The mericarps of Coriandrum sativum L. (coriander) produce an essential oil (EO) rich in monoterpenes, volatile C10 terpenoids. To investigate EO metabolism, the transcriptome of coriander mericarps, at three developmental stages (early, mid, late) was sequenced via Illumina technology and a transcript library was produced. To validate the usability of the transcriptome sequences, two terpene synthase candidate genes, CsγTRPS and CsLINS, encoding 558 and 562 amino acid proteins were expressed in bacteria, and the recombinant proteins purified by Ni-NTA affinity chromatography. The 65.16 (CsγTRPS) and 65.91 (CsLINS)kDa recombinant proteins catalyzed the conversion of geranyl diphosphate, the precursor to monoterpenes, to γ-terpinene and (S)-linalool, respectively, with apparent Vmax and Km values of 2.24±0.16 (CsγTRPS); 19.63±1.05 (CsLINS)pkat/mg and 66.25±13 (CsγTRPS); 2.5±0.6 (CsLINS)μM, respectively. Together, CsγTRPS and CsLINS account for the majority of EO constituents in coriander mericarps. Investigation of the coriander transcriptome, and knowledge gained from these experiments will facilitate future studies concerning essential and fatty acid oil production in coriander. They also enable efforts to improve the coriander oils through metabolic engineering or plant breeding.

  20. STUDY OF ANTIDEPRESSANT LIKE EFFECT OF CORIANDRUM SATIVUM AND INVOLVEMENT OF MONOAMINONERGIC AND GABANERGIC SYSTEM

    OpenAIRE

    Naikwade Nilofer; Gumate Deepak; Kokane Sushant; Patil Vipul; Kharade Sudha

    2011-01-01

    The aim of this study was to examine possible mechanism of action of aqueous extract of Coriandrum sativum seed central nervous system of mice. We investigated the antidepressant-like mechanism of Coriandrum sativum by the combination of the Sulpiride (a selective dopamine D2 receptor antagonist), Prazosin (a α1 adrenoceptor antagonist), and Baclofen (GABA agonist). The results show that Coriandrum sativum (200 mg/kg, 400mg/kg, p.o.), significantly reduced the immobility time during Tail Susp...

  1. Antioxidant potential of Coriandrum sativum L. seed extract.

    Science.gov (United States)

    Deepa, B; Anuradha, C V

    2011-01-01

    The seeds of C. sativum are used as a traditional drug for the treatment of diabetes. The antioxidant and free-radical-scavenging property of seeds in vitro was studied and also investigated whether the administration of seeds curtails oxidative stress in the kidney of streptozotocin-induced diabetic rats. Incorporation of seed powder in the diet led to marked lowering of blood glucose and a rise in the levels of insulin in diabetic rats. A parallel beneficial effect was observed on oxidant -antioxidant balance in the kidney. Addition of coriander seed powder not only inhibited the process of peroxidative damage but also significantly reactivated the antioxidant enzymes and antioxidant levels in diabetic rats. The total polyphenolic content of the seeds was found to be 12.2 gallic acid equivalents (GAE)/g while total flavanoid content was found to be 12.6 quercetin equivalents/g. The seeds also showed scavenging activity against superoxides and hydroxyl radicals in a concentration-dependent manner. Maximum free radical-scavenging action and free radical reducing power of coriander seed extract was observed at a concentration of 50 microg GAE. Islet histology structures showed degeneration of pancreatic islets in diabetic rats which was also reduced in diabetic rats treated with seed powder. These results show that C. sativum seeds not only possess antihyperglycemic properties but antioxidative properties also. Increased dietary intake of coriander seeds decrease the oxidative burden in diabetes mellitus.

  2. In vitro and in vivo anthelmintic activity of seed extract of Coriandrum sativum compared to Niclosamid against Hymenolepis nana infection.

    Science.gov (United States)

    Hosseinzadeh, Samaneh; Ghalesefidi, Maryam Jamshidian; Azami, Mehdi; Mohaghegh, Mohammad Ali; Hejazi, Seyed Hossein; Ghomashlooyan, Mohsen

    2016-12-01

    Phytotherapy can be an alternative for the control of gastrointestinal parasites in human and animals. Coriander (Coriandrum sativum L.) is a medicinal plant which grown as a spice crop all over the world. The seeds of this plant have been used to treat parasitic disease, indigestion, diabetes, rheumatism and pain in the joints. This study was carried out to compare the efficacy of Niclosamid and alcoholic seed extract of C. sativum on Hymenolepis nana infection, in vivo and vitro. For in vivo study, Balb/c mice were used, to compare the efficacy of 50 mg/kg body weight (B.W) of Niclosamid with different doses of alcoholic extracts of C. sativum (250, 500, and 750 mg/kg B.W). It was found that the efficacy of Niclosamid had reached 100 % after 11 days post treatment, while the efficacy of 500 and 750 mg/kg B.W of C. sativum reached to 100 % after 15 days after treatment. For in vitro study, special nutrient broth media was used. It was found that the addition of 1000 mg/ml of Niclosamid had paralyzed and killed worms within 5 min, while C. sativum killed them within 30 min. Our results showed that extract of C. sativum has good effect against H. nana and could be use in traditional medicine for treatment of parasitic disease.

  3. Rendimento de coentro (Coriandrum sativum L. em sistema de adubação verde com a planta jitirana (Merremia aegyptia L. Yield of coriander (Coriandrum sativum L. in a system of green manure with the plant scarlet starglory (Merremia aegyptia L.

    Directory of Open Access Journals (Sweden)

    P.C.F. Linhares

    2012-01-01

    Full Text Available O coentro é a hortaliça mais utilizada como condimento na região de Mossoró-RN. Um experimento foi conduzido na Fazenda Experimental Rafael Fernandes da Universidade Federal Rural do Semi-Árido - UFERSA, no período de maio a junho de 2010, com o objetivo de avaliar o rendimento de coentro sob diferentes quantidades da planta trepadeira jitirana, incorporada ao solo como adubo verde. O delineamento experimental usado foi o de blocos completos casualizados com sete tratamentos e três repetições. Os tratamentos consistiram da incorporação de sete quantidades de jitirana (3,0; 6,0; 9,0; 12,0; 15,0; 18,0 e 21,0 t ha-1 de matéria seca de jitirana. A cultivar de coentro plantado foi a Verdão. As características avaliadas foram: altura e número de hastes por planta, rendimento e massa seca da parte aérea. O melhor desempenho agronômico do coentro foi observado na quantidade de 21,0 t ha-1 de jitirana. Para cada tonelada de jitirana incorporada ao solo observa-se um rendimento de coentro de 395 kg ha-1.The ciliandro is the vegetable more used as seasoning in the area at Mossoró-RN. An experiment was conducted at the Experimental Farm Rafael Fernandes of the Universidade Federal Rural do Semi-Árido - UFERSA in the period from may to june 2010, with the objective of evaluating the coriander yield in under different amounts of plant clambering scarlet starglory, incorporated into the soil. The experimental design was randomized complete blocks with eight treatments and three replicates. The treatments were combinations of eight amounts of scarlet starglory (3.0; 6.0; 9.0; 12.0; 15.0; 18.0 and 21.0 t ha-1 dry matter. The coriander cultivar planted was Verdão. The characteristics evaluated in the coriander were: plant height and number of stalks per plant, yield and dry matter mass of shoots. The best agroconomic performance lettuce was observed in the amount of 21,0 t ha-1 scarlet starglory. For each fresh or dry scarlet starglory ton

  4. STUDY OF ANTIDEPRESSANT LIKE EFFECT OF CORIANDRUM SATIVUM AND INVOLVEMENT OF MONOAMINONERGIC AND GABANERGIC SYSTEM

    Directory of Open Access Journals (Sweden)

    Naikwade Nilofer

    2011-02-01

    Full Text Available The aim of this study was to examine possible mechanism of action of aqueous extract of Coriandrum sativum seed central nervous system of mice. We investigated the antidepressant-like mechanism of Coriandrum sativum by the combination of the Sulpiride (a selective dopamine D2 receptor antagonist, Prazosin (a α1 adrenoceptor antagonist, and Baclofen (GABA agonist. The results show that Coriandrum sativum (200 mg/kg, 400mg/kg, p.o., significantly reduced the immobility time during Tail Suspension Test (TST. We also investigated the antidepressant-like mechanism of Coriandrum sativum by the combination of Sulpiride (a selective dopamine D2 receptor antagonist, Prazosin (a α1 adrenoceptor antagonist, and Baclofen (GABA agonist. The immobility time after treatment with Coriandrum sativum (200 mg/kg, 400mg/kg, p.o. in TST was augmented by Sulpiride, Baclofen, Prazosin. Our findings support the view that Coriandrum sativum exerts antidepressant-like effect. And the mechanism of action of Coriandrum sativum may be related to the increase in Nor adrenaline and serotonin levels in the hippocampus and frontal cortex.

  5. In vitro and in vivo anthelmintic activity of crude extracts of Coriandrum sativum against Haemonchus contortus.

    Science.gov (United States)

    Eguale, T; Tilahun, G; Debella, A; Feleke, A; Makonnen, E

    2007-04-04

    In vitro anthelmintic activities of crude aqueous and hydro-alcoholic extracts of the seeds of Coriandrum sativum (Apiaceae) were investigated on the egg and adult nematode parasite Haemonchus contortus. The aqueous extract of Coriandrum sativum was also investigated for in vivo anthelmintic activity in sheep infected with Haemonchus contortus. Both extract types of Coriandrum sativum inhibited hatching of eggs completely at a concentration less than 0.5 mg/ml. ED(50) of aqueous extract of Coriandrum sativum was 0.12 mg/ml while that of hydro-alcoholic extract was 0.18 mg/ml. There was no statistically significant difference between aqueous and hydro-alcoholic extracts (p>0.05). The hydro-alcoholic extract showed better in vitro activity against adult parasites than the aqueous one. For the in vivo study, 24 sheep artificially infected with Haemonchus contortus were randomly divided into four groups of six animals each. The first two groups were treated with crude aqueous extract of Coriandrum sativum at 0.45 and 0.9 g/kg dose levels, the third group with albendazole at 3.8 mg/kg and the last group was left untreated. Efficacy was tested by faecal egg count reduction (FECR) and total worm count reduction (TWCR). On day 2 post treatment, significant FECR was detected in groups treated with higher dose of Coriandrum sativum (pCoriandrum sativum (p>0.05). Significant (pCoriandrum sativum compared to the untreated group. Reduction in male worms was higher than female worms. Treatment with both doses of Coriandrum sativum did not help the animals improve or maintain their PCV while those treated with albendazole showed significant increase in PCV (p<0.05).

  6. Genome sequence of vanilla distortion mosaic virus infecting Coriandrum sativum.

    Science.gov (United States)

    Adams, I P; Rai, S; Deka, M; Harju, V; Hodges, T; Hayward, G; Skelton, A; Fox, A; Boonham, N

    2014-12-01

    The 9573-nucleotide genome of a potyvirus was sequenced from a Coriandrum sativum plant from India with viral symptoms. On analysis, this virus was shown to have greater than 85 % nucleotide sequence identity to vanilla distortion mosaic virus (VDMV). Analysis of the putative coat protein sequence confirmed that this virus was in fact VDMV, with greater than 91 % amino acid sequence identity. The genome appears to encode a 3083-amino-acid polyprotein potentially cleaved into the 10 mature proteins expected in potyviruses. Phylogenetic analysis confirmed that VDMV is a distinct but ungrouped member of the genus Potyvirus.

  7. Effect of ethanolic extract of Coriandrum sativum L. on tacrine induced orofacial dyskinesia.

    Science.gov (United States)

    Mohan, Mahalaxmi; Yarlagadda, Sanjyothi; Chintala, Saritha

    2015-05-01

    The effect of ethanolic extract of Coriandrum sativum L. seeds (100, 200 mg/kg) was studied on tacrine induced orofacial dyskinesia. Tacrine (2.5 mg/kg, i.p.) treated animals were observed for vacuous chewing movements (VCM), tongue protrusions (TP) and orofacial bursts (OB) for 1 h followed by observations for locomotor changes and cognitive dysfunction. Sub-chronic administration of Coriandrum sativum L. seed extract (E-CS) (100, 200 mg/kg, p.o., for 15 days significantly (P Coriandrum sativum. L against tacrine induced orofacial dyskinesia.

  8. In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract.

    Science.gov (United States)

    Harsha, S N; Anilakumar, K R

    2014-08-01

    Coriandrum sativum L. (coriander), an everyday spice in the Indian kitchen is known to add flavor to the cuisine. It is an annual herb belonging to the Apiaceae (Umbellifera) family. The hydro-alcohol extract of Coriandrum sativum L. at the dose of 1 mg/ml was subjected to a series of in vitro assays viz. 2, 2'- diphenyl-1-picrylhydrazyl, lipid peroxidation by thiobarbituric acid, reducing power and nitric oxide (NO) radical scavenging in order to study its antioxidant efficacy in detail. The amount of flavonoids in 70% ethanol extract was found to be 44.5 μg and that of the total phenols was 133.74 μg gallic acid equivalents per mg extract. The extracts of the leaves showed metal chelating power, with IC50 values, 368.12 μg/ml where as that of standard EDTA was 26.7 μg/ml. The IC50 values for 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid radical scavenging was 222 μg/ml where as that of standard ascorbic acid was 22.6 μg/ml. The NO scavenging activity of the extract of the leaves showed IC50 value of 815.6 μg/ml; at the same time the standard BHA had 49.1 μg/ml. All the plant extracts provided DNA damage protection; however, the protection provided at the dose of 8 μg/ml was comparable to that of standard gallic acid. The Coriandrum sativum leaf extract was able to prevent in vitro lipid peroxidation with IC50 values; 589.6 μg/ml where as that of standard BHA was 16.3 μg/ml. Our results also showed significant ferric reducing power indicating the hydrogen donating ability of the extract. This study indicated the potential of the leaf extract as a source of natural antioxidants or nutraceuticals that could be of use in food industry with potential application to reduce oxidative stress in living system.

  9. Synthesis and physical properties of coriander estolide 2-ethylhexyl esters

    Science.gov (United States)

    Coriander (Coriandrum sativum L.) is new crop that is currently being investigated for cultivation in Central Illinois by USDA-NCAUR. Coriander is an annual herb belonging to the Apiaceae (Umbelliferae) family and indigenous to the Mediterranean basin areas and the Near East. This plant is widely ...

  10. 天然橡胶初加工废水灌溉对芫荽生物量及品质的影响%Effects of the Primary Processing of Natural Rubber Wastewater Irrigation on Biomass and Quality of Coriander (Coriandrum sativum)

    Institute of Scientific and Technical Information of China (English)

    黄亚文; 张旭; 刘坤; 唐文浩

    2012-01-01

    [目的]用天然橡胶初加工废水灌溉芫荽(Coriandrum sativum),研究其对芫荽生物量及品质的影响.[方法]采用盆栽试验法研究不同稀释度天然橡胶初加工废水(100% 、50% 、25%、10%胶清废水,100% 、50% 、25%、10% UASB厌氧出水,好氧生物处理终水)对芫荽生物量及品质的影响.[结果]与清水浇灌相比,未稀释的胶清废水灌溉对芫荽的生长有强烈的负面影响,其余废水处理对芫荽的生物量及品质均有一定的促进作用;在不同稀释度的胶清废水灌溉中,浓度10%的胶清废水的促进作用最明显,在地上部分干重、地下部分干重、总叶绿素含量、维生素C含量、可溶性总糖含量方面分别比清水浇灌高出281.44% 、33.18%、20.69%、5.66%、212.52%;在不同稀释度的UASB厌氧出水灌溉中,浓度50%的UASB厌氧出水的促进作用最明显,在地上部分干重、地下部分干重、总叶绿素含量、维生素C含量、可溶性总糖含量方面分别比清水浇灌高出284.43% 、58.16%、16.47% 、16.59% 、146.62%;好氧生物处理终水处理的地上部分干重、地下部分干重、总叶绿素含量、维生素C含量、可溶性总糖含量方面分别比清水浇灌高出216.29%、17.83%、7.11%、2.15%、63.67%;好氧生物处理终水直接灌溉芫荽,其地上部分硝酸盐含量符合国家标准(GB 19338-2003)限值,胶清废水和UASB厌氧出水灌溉存在硝酸盐积累问题.[结论]该研究可为天然橡胶初加工废水的无害化和资源化利用提供指导.%[ Objective] The primary processing of natural rubber wastewater was used to irrigate coriander, and then study their effects on coriander & biomass and quality. [ Method] A pot experiment was conducted to study the effects of irrigation with different dilutions of the primary processing of natural rubber wastewater irrigation on the biomass and quality of coriander. The

  11. Comparison of essential oil composition and antimicrobial activity of Coriandrum sativum L. extracted by hydrodistillation and microwave-assisted hydrodistillation.

    Science.gov (United States)

    Sourmaghi, Mohammad Hossein Salehi; Kiaee, Gita; Golfakhrabadi, Fereshteh; Jamalifar, Hossein; Khanavi, Mahnaz

    2015-04-01

    Coriander (Coriandrum sativum L.), is an annual herb in the Apiaceae family which disperses in Mediterranean and Middle Eastern regions. The Coriander essential oil has been used in food products, perfumes, cosmetics and pharmaceutical industries for its flavor and odor. In Iran, fruits of Coriander used in pickle, curry powders, sausages, cakes, pastries, biscuits and buns. The aim of this study was to investigate microwave radiation effects on quality, quantity and antimicrobial activity of essential oil of Coriander fruits. The essential oils were obtained from the Coriander fruits by hydrodistillation (HD) and Microwave-assisted hydrodistillation (MAHD) then, the oils were analyzed by GC and GC-MS. Antimicrobial activities of essential oils were evaluated against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans by microdilution method. The results indicated that the HD and MAHD essential oils (EO) were dominated by monoterpenoids such as linalool, geranyl acetate and γ-terpinene. The major compound in both EO was linalool which its amount in HD and MAHD was 63 % and 66 %, respectively. The total amount of monoterpenes hydrocarbons in HD EO differ significantly with the amount in MAHD EO (12.56 % compare to 1.82 %). HD EO showed greater activity against Staphylococcus aureus and Candida albicans than MAHD EO. Moreover, their activities against Ecoli and P. aeruginosa were the same with Minimum Inhibitory Concentration (MIC) 0.781 and 6.25 μL mL(-1), respectively. By using MAHD method, it was superior in terms of saving energy and extraction time, although the oil yield and total composition decrease by using this method.

  12. Coupled extruder-headspace, a new method for analysis of the essential oil components of Coriandrum sativum fruits.

    Science.gov (United States)

    Sriti, Jazia; Msaada, Kamel; Talou, Thierry; Faye, Mamadou; Vilarem, Gerard; Marzouk, Brahim

    2012-10-15

    A new method involving concurrent single screw extruder combined with continuous headspace dynamic for the extraction and identification of the essential oil of Coriandrum sativum L. fruit was developed. The effect of six different nozzle diameters (5, 6, 7, 8, 9 and 10 mm) on the content and chemical composition of the essential oil of coriander fruit was studied. The oils from fruit samples were obtained by OMEGA 20 extruder. The result showed that the highest yield (0.53%) was obtained by the diameter of the nozzle was 8mm. Twenty-nine components were determined in essential oils, which were mostly hydrocarbons and alcohol monoterpenes. The main components linalool, α-pinene, γ-terpinene, p-cymene and limonene showed significant variations with drying trials.

  13. Anti-granuloma activity of Coriandrum sativum in experimental models

    Directory of Open Access Journals (Sweden)

    Vinod Nair

    2013-01-01

    Full Text Available Background: Coriandrum sativum has been used in the traditional systems of medicine for management of arthritis and other inflammatory disorders. Objectives: In this study, we have evaluated the anti-inflammatory and anti-granuloma activities of Coriandrum sativum hydroalcoholic extract (CSHE in experimental models. Materials and Methods: The anti-inflammatory activity of CSHE was evaluated using carrageenan-induced paw edema model and the anti-granuloma activity of CSHE was evaluated using the subcutaneous cotton pellet implantation-induced granuloma formation and stimulation of peritoneal macrophages with complete Freund′s adjuvant. Serum tumor necrosis factor-α (TNF-α, IL-6, IL-1 β levels, and peritoneal macrophage expression of TNF-R1 were evaluated as markers of global inflammation. Results: CSHE at the highest dose tested (32 mg/kg produced a significant reduction (P < 0.05 in paw edema after carrageenan administration. CSHE treatment also reduced dry granuloma weight in all treated animals. Serum IL-6 and IL-1 β levels were significantly ( P < 0.05 lower in the CSHE (32 mg/kg-treated group as compared to control. Although there was an increase in serum TNF-α level in the CSHE-treated group as compared to control, TNF-R1 expression on peritoneal macrophages was found to be reduced. Conclusion: Thus, the result of this study demonstrates the anti-inflammatory and anti-granuloma activities of CSHE in experimental models, and validates its traditional use for the management of arthritis and other inflammatory disorders.

  14. The effect of Coriandrum sativum seed extract on the learning of newborn mice by electric shock: interaction with caffeine and diazepam.

    Science.gov (United States)

    Zargar-Nattaj, Seyed Sadegh; Tayyebi, Pooya; Zangoori, Vahid; Moghadamnia, Yasaman; Roodgari, Hasan; Jorsaraei, Seyed Gholamali; Moghadamnia, Ali Akbar

    2011-01-01

    Coriander has been recommended for the relief of pain, anxiety, flatulence, and loss of appetite. In traditional medicine, it is believed that coriander can induce some degree of amnesia in a child when his/her mother uses coriander during the pregnancy. We evaluated the effect of Coriandrum sativum seed extract on learning in second-generation mice. Ethanolic extract (2%) of coriander (100 mg/kg intraperitoneal) was dissolved in sunflower oil (oil) as a vehicle and injected into the control group mother mice during breastfeeding for 25 days at 5-day intervals. After feeding the newborn mice, their learning was evaluated using a step-through passive avoidance task with 0.4 mA electric shock for 2 or 4 seconds. While coriander extract showed a negative effect in the short term (1 hour) after the training session, it potentiated the mice's learning in later assessments (24 hours post-training [P = 0.022] and 1 week post-training [P = 0.002] by a 4-second shock). Low-dose caffeine (25 mg/kg ip after training) improved the learning after 1 hour (P = 0.024); while diazepam (1 mg/kg ip) suppressed learning at all time points after the 4-second shock training (1 hour, P = 0.022; 24 hours, P = 0.002; and 1 week, P = 0.008). No modification in the pain threshold was elicited by electric stimuli both in coriander and control groups. In conclusion, coriander does not improve learning within a short period of time after training; however, learning after coriander administration can be improved in the long term.

  15. The effect of Coriandrum sativum seed extract on the learning of newborn mice by electric shock: interaction with caffeine and diazepam

    Directory of Open Access Journals (Sweden)

    Seyed Sadegh Zargar-Nattaj

    2011-01-01

    Full Text Available Seyed Sadegh Zargar-Nattaj1, Pooya Tayyebi1, Vahid Zangoori1, Yasaman Moghadamnia4, Hasan Roodgari2, Seyed Gholamali Jorsaraei3, Ali Akbar Moghadamnia11Department of Pharmacology, 2Department of Medical Genetics, 3Department of Anatomical Sciences and Embryology, Babol University of Medical Sciences, Babol, Iran; 4Department of Physics, Alzzahra University, Tehran, IranAbstract: Coriander has been recommended for the relief of pain, anxiety, flatulence, and loss of appetite. In traditional medicine, it is believed that coriander can induce some degree of amnesia in a child when his/her mother uses coriander during the pregnancy. We evaluated the effect of Coriandrum sativum seed extract on learning in second-generation mice. Ethanolic extract (2% of coriander (100 mg/kg intraperitoneal was dissolved in sunflower oil (oil as a vehicle and injected into the control group mother mice during breastfeeding for 25 days at 5-day intervals. After feeding the newborn mice, their learning was evaluated using a step-through passive avoidance task with 0.4 mA electric shock for 2 or 4 seconds. While coriander extract showed a negative effect in the short term (1 hour after the training session, it potentiated the mice's learning in later assessments (24 hours post-training [P = 0.022] and 1 week post-training [P = 0.002] by a 4-second shock. Low-dose caffeine (25 mg/kg ip after training improved the learning after 1 hour (P = 0.024; while diazepam (1 mg/kg ip suppressed learning at all time points after the 4-second shock training (1 hour, P = 0.022; 24 hours, P = 0.002; and 1 week, P = 0.008. No modification in the pain threshold was elicited by electric stimuli both in coriander and control groups. In conclusion, coriander does not improve learning within a short period of time after training; however, learning after coriander administration can be improved in the long term.Keywords: Coriandrum sativum, caffeine, diazepam, learning, memory, step through

  16. Effect of Coriandrum sativum hydroalcoholic extract and its essential oil on acetic acid- induced acute colitis in rats

    Directory of Open Access Journals (Sweden)

    Bahareh Heidari

    2016-03-01

    Full Text Available Objective: The aim of this study was to determine the protective effects of Coriandrum sativum on acetic acid-inducedcolitis in rats. C. sativum (Coriander has long been used in Iranian traditional medicine and its use as an anti-inflammatory agent is still common in some herbal formulations.  Materials and Methods: Colitis was induced by intra-rectal administration of 2ml acetic acid 4% in fasted male Wistar rats. Treatment was carried out using three increasing doses of extract (250, 500, 1000 mg/kg and essential oil (0.25, 0.5, 1 ml/kg of coriander started 2 h before colitis induction and continued for a five-day period. Colon biopsies were taken for weighting, macroscopic scoring of injured tissue, histopathological examination and measuring myeloperoxidase (MPO activity.  Results: Colon weight was decreased in the groups treated with extract (500 and 1000 mg/kg and essential oil (0.5 ml/kg compared to the control group. Regarding MPO levels, ulcer severity and area as well as the total colitis index, same results indicating meaningful alleviation of colitis was achieved after treatment with oral extract and essential oil.  Conclusion: Since the present experiment was made by oral fractions of coriander thus the resulting effects could be due to both the absorption of the active ingredients and/or the effect of non-absorbable materials on colitis after reaching the colon. In this regard, we propose more toxicological and clinical experiments to warranty its beneficial application in human inflammatory bowel diseases.

  17. Plant regeneration through somatic embryogenesis and genome size analysis of Coriandrum sativum L.

    Science.gov (United States)

    Ali, Muzamil; Mujib, A; Tonk, Dipti; Zafar, Nadia

    2017-01-01

    In the present study, an improved plant regeneration protocol via primary and secondary somatic embryogenesis was established in two Co-1 and Rajendra Swathi (RS) varieties of Coriandrum sativum L. Callus was induced from root explants on 2, 4-D (0.5-2.0 mg/l) supplemented MS. The addition of BA (0.2 mg/l) improved callus induction and proliferation response significantly. The maximum callus induction frequency was on 1.0 mg/l 2, 4-D and 0.2 mg/l BA added MS medium (77.5 % in Co-1 and 72.3 % in RS). The callus transformed into embryogenic callus on 2, 4-D added MS with maximum embryogenic frequency was on 1.0 mg/l. The granular embryogenic callus differentiated into globular embryos on induction medium, which later progressed to heart-, torpedo- and cotyledonary embryos on medium amended with 0.5 mg/l NAA and 0.2 mg/l BA. On an average, 2-3 secondary somatic embryos (SEs) were developed on mature primary SEs, which increased the total embryo numbers in culture. Histology and scanning electron microscopy (SEM) studies are presented for the origin, development of primary and secondary embryos in coriander. Later, these induced embryos converted into plantlets on 1.0 mg/l BA and 0.2 mg/l NAA-amended medium. The regenerated plantlets were cultured on 0.5 mg/l IBA added ½ MS for promotion of roots. The well-rooted plantlets were acclimatized and transferred to soil. The genetic stability of embryo-regenerated plant was analyzed by flow cytometry with optimized Pongamia pinnata as standard. The 2C DNA content of RS coriander variety was estimated to 5.1 pg; the primary and secondary somatic embryo-derived plants had 5.26 and 5.44 pg 2C DNA content, respectively. The regenerated plants were genetically stable, genome size similar to seed-germinated coriander plants.

  18. Processing of coriander fruits for the production of essential oil, triglyceride, and high protein seed meal

    Science.gov (United States)

    Coriander (Coriandrum sativum L.) is a summer annual traditionally grown for use as a fresh green herb or as a spice. The essential oil extracted from coriander fruit is also widely used as flavoring in a variety of food products. The fatty oil (triglyceride) fraction in the seed is rich in petrosel...

  19. Dehulling of coriander fruit before oil extraction

    Science.gov (United States)

    Coriander (Coriandrum sativum L.) is a summer annual traditionally grown for use as fresh green herb, spice or for its essential oil. The essential oil is obtained by steam distillation of crushed fruit and the residue is utilized as feed or processed further to recover the triglyceride. The triglyc...

  20. Effects of coriander (Coriandrum sativum L.) seed powder and ...

    African Journals Online (AJOL)

    david

    2015-09-03

    Sep 3, 2015 ... herbs and spices and their active compounds act as food condiments through the oronasal (oral and nasal .... Financial support by Islamic Azad University, Rasht Branch, Iran, grant no 4.5830 for is .... Trends Food Sci.

  1. Prevalence of cilantro (Coriandrum sativum disliking among different ethnocultural groups

    Directory of Open Access Journals (Sweden)

    Mauer Lilli

    2012-05-01

    Full Text Available Abstract Background Cilantro, the leaf of the Coriandrum sativum plant, is an herb that is widely consumed globally and has purported health benefits ranging from antibacterial to anticancer activities. Some individuals report an extreme dislike for cilantro, and this may explain the different cilantro consumption habits between populations. However, the prevalence of cilantro dislike has not previously been reported in any population. The objective of this study was to determine the prevalence of cilantro dislike among different ethnocultural groups from a population of young adults living in Canada. Subjects (n = 1,639 between the ages of 20 and 29 years were participants of the Toronto Nutrigenomics and Health Study. Individuals rated their preference for cilantro on a 9-point scale from ‘dislike extremely’ to ‘like extremely’. Subjects also had the option to select ‘have not tried’ or ‘would not try’. Subjects who selected 1 to 4 were classified as disliking cilantro. Results The prevalence of dislike ranged from 3 to 21%. The proportion of subjects classified as disliking cilantro was 21% for East Asians, 17% for Caucasians, 14% for those of African descent, 7% for South Asians, 4% for Hispanics, and 3% for Middle Eastern subjects. Conclusions These findings show that the prevalence of cilantro dislike differs widely between various ethnocultural groups.

  2. New Bioactive Oleanane Type Compounds from Coriandrum sativum Linn.

    Directory of Open Access Journals (Sweden)

    Muhammad Iqbal

    2014-01-01

    Full Text Available Five (1–5 new bioactive oleanane type triterpenoids have been isolated from ethyl acetate soluble fraction of ethanolic extract of Coriandrum sativum Linn. of Umbelliferae family. Ethanolic extract of the whole plant was fractionated in organic solvents. Ethyl acetate fraction was subjected to column chromatography on HPLC RP-18 to get 1-oxo-11β,21β-dihydroxy-oleanane (1, 1-oxo-11β-hydroxy-21β-O-acetyloleanane (2, 1-oxo-11β-hydroxy-21β-O-angeloyloleanane (3, 1-oxo-11β-O-angeloyl-21β-O-acetyloleanane (4, and 1-oxo-11β,21β-O-dibenzoyloleanane (5. The structures were elucidated after analysis of spectroscopic data, UV, IR, NMR (1H, 13C, 1D, and 2D, and mass measurements. Suspension in water of crude ethyl acetate extract was employed to treat sheep with ringworm disease. All isolated compounds (1–5 displayed excellent activity in terms of inhibition zones, MICs, MBCs, and MFCs against both bacteria and fungi. Ethyl acetate extract showed excellent antiringworm activity in sheep.

  3. Larvicidal and repellent activity of the essential oil of Coriandrum sativum L. (Apiaceae) fruits against the filariasis vector Aedes albopictus Skuse (Diptera: Culicidae).

    Science.gov (United States)

    Benelli, Giovanni; Flamini, Guido; Fiore, Giulia; Cioni, Pier Luigi; Conti, Barbara

    2013-03-01

    The essential oils of many Apiaceae species have been already studied for their insecticidal and repellent properties against insect pests. In this research, the essential oil (EO) extracted from the fruits of Coriandrum sativum L. (Apiaceae) was evaluated for the first time for its larvicidal and repellent activities against the most invasive mosquito worldwide, Aedes albopictus Skuse (Diptera: Culicidae). The chemical composition of C. sativum EO was investigated by gas chromatography with electron impact mass spectrometry analysis. Coriander EO was mainly composed by monoterpene hydrocarbons and oxygenated monoterpenes, with linalool (83.6 %) as the major constituent. C. sativum EO exerted toxic activity against A. albopictus larvae: LC(50) was 421 ppm, while LC(90) was 531.7 ppm. Repellence trials highlighted that C. sativum EO was a good repellent against A. albopictus, also at lower dosages: RD(50) was 0.0001565 μL/cm(2) of skin, while RD(90) was 0.002004 μL/cm(2). At the highest dosage (0.2 μL/cm(2) of skin), the protection time achieved with C. sativum essential oil was higher than 60 min. This study adds knowledge about the chemical composition of C. sativum EO as well as to the larvicidal and repellent activity exerted by this EO against A. albopictus. On this basis, we believe that our findings could be useful for the development of new and safer products against the Asian tiger mosquito.

  4. Synthesis and physical properties of estolides 2-ethylhexyl esters from coriander fatty acids capped with various fatty acids

    Science.gov (United States)

    Coriander (Coriandrum sativum L.) is a new crop that is currently being cultivated in Central Illinois by USDA-NCAUR. Coriander is an annual herb belonging to the Apiaceae (Umbelliferae) family and indigenous to the Mediterranean basin areas and the Near East. This plant is widely distributed and ...

  5. Chemical Composition and Insecticidal Activity of Essential Oil from Coriandrum sativum Seeds against Tribolium confusum and Callosobruchus maculatus.

    Science.gov (United States)

    Khani, Abbas; Rahdari, Tahere

    2012-01-01

    The biological activity of essential oil extracted from coriander, Coriandrum sativum L. (Apiaceae), seeds against adults of Tribolium confusum Duval (Coleoptera: Tenebrionidae) and Callosobruchus maculatus F. (Coleoptera: Bruchidae) was investigated in a series of laboratory experiments. Fumigant toxicity was assessed at 27 ± 1°C and 65 ± 5% R.H., in dark condition. Dry seeds of the plant were subject to hydrodistillation using a Clevenger-type apparatus. The composition of essential oil was analyzed by gas chromatography mass spectrometry. The predominant components in the oil were linalool (57.57%) and geranyl acetate (15.09%). The mortality of 1-7-day-old adults of the insect pests increased with concentration from 43 to 357 μL/L air and with exposure time from 3 to 24 h. In the probit analysis, LC(50) values (lethal concentration for 50% mortality) showed that C. maculatus (LC(50) = 1.34 μL/L air) was more susceptible than T. confusum (LC(50) = 318.02 μL/L air) to seed essential oil of this plant. The essential oil of C. sativum can play an important role in stored grain protection and reduce the risks associated with the use of synthetic insecticides.

  6. Impact of cerium oxide nanoparticles on cilantro ( Coriandrum sativum)

    Science.gov (United States)

    Morales, Maria Isabel

    Studies have shown that plants exposed to ENPs suffer different types of stress. Other studies have revealed that plants can take up and accumulate CeO2 NPs without modification. Thus, these NPs could enter the food chain through edible plants, posing a threat for human health. Cilantro (Coriandrum sativum) is a worldwide culinary and medicinal plant consumed either as a fresh herb or a spice. In this research, cilantro plants were germinated and cultivated for 30 days in organic soil treated with CeO2 NPs at concentrations varying from 0 to 500 mg kg -1. Subsequently, plant organs were analyzed by using spectroscopic techniques and biochemical assays. Results indicate that at 125 mg kg -1, the CeO2 NPs significantly increased the root size compared with the other treatments. The ICP-OES results showed that plants exposed to 500 mg kg-1 had significantly (p ≤ 0.05) more Ce in shoots and roots compared to the other treatments. Results from the biochemical assays showed that at 125 mg kg-1, catalese activity significantly increased in shoots and ascorbate peroxidase in roots (p ≤ 0.05). In addition, the FTIR analyses revealed that at 125 mg kg-1, the CeO2 NPs changed the chemical environment of the carbohydrates within the cilantro shoots, for which changes in the area of the stretching frequencies were observed. Moreover, analyses of antioxidant compounds showed a significant ( p ≤ 0.05) reduction on total phenolic content in shoots of cilantro plants treated with 500 mg CeO2 NPs kg-1 . This suggests that the CeO2 NPs have the potential to diminish the ability of cilantro plants to scavenge reactive oxygen species. The multi-elemental analysis showed that plants treated with CeO2 at the 500 mg kg-1 treatment had a significant ( p ≤ 0.05) reduction in shoots' sulfur, silicon, and zinc accumulation. The results of this research indicate that the CeO2 NPs at 500 mg CeO2 kg-1 concentration cause a reduction in the antioxidant ability and nutritional properties

  7. EVALUATION OF THE CHELATING EFFECT OF METHANOLIC EXTRACT OF CORIANDRUM SATIVUM AND ITS FRACTIONS ON WISTAR RATS POISONED WITH LEAD ACETATE.

    Science.gov (United States)

    Téllez-López, Miguel Ángel; Mora-Tovar, Gabriela; Ceniceros-Méndez, Iromi Marlen; García-Lujan, Concepción; Puente-Valenzuela, Cristo Omar; Vega-Menchaca, María Del Carmen; Serrano-Gallardo, Luis Benjamín; Garza, Rubén García; Morán-Martínez, Javier

    2017-01-01

    The rate of lead poisoning has decreased in recent years due to increased health control in industries that use this metal. However, it is still a public health problem worldwide. The use of various plants with chelating properties has been a topic of research today. In traditional medicine, it is said that Coriandrum sativum has chelating properties, but there is no scientific evidence to support this fact. The purpose of this research is to evaluate the chelating effect of methanol extract of coriander and its fractions on Wistar rats intoxicated with lead. In this research, male Wistar rats were poisoned with 50 mg/kg of lead acetate and treated with 50 mg/kg of methanol extract and its fractions. The extract and its fractions were administered to four treatment groups. Positive and negative controls were established. Hemoglobin, hematocrit and lead concentrations were analyzed; liver was evaluated histologically in control and treatment groups. The methanol extract of coriander presented a LD50 >1000 mg/dL. The group administered with the methanol extract showed significant difference in the levels of hemoglobin and hematocrit compared to the negative control group. Lead concentration in treatment groups showed a decrease compared to the positive control. Histological evaluation of tissue showed less damage in groups administered with methanolic extract and its fractions compared to the positive control which presented structural alterations. Coriander extracts protect liver and lower lead concentration in rats intoxicated with lead in contrast to the positive control group.

  8. Antibacterial activity of Phyllantus emblica, Coriandrum sativum, Culinaris medic, Lawsonia alba and Cucumis sativus.

    Science.gov (United States)

    Khan, Dawood Ali; Hassan, Fouzia; Ullah, Hanif; Karim, Sabiha; Baseer, Abdul; Abid, Mobasher Ali; Ubaidi, Muhammad; Khan, Shujaat Ali; Murtaza, Ghulam

    2013-01-01

    Present study deals with the demonstration of the antibacterial activity of very common medicinal plants of Pakistani origin i.e., Phyllantus emblica, Coriandrum sativum, Culinaris medic, Lawsonia alba and Cucumis sativus. The extracts were prepared in crude form by the use of hydro-alcoholic solution and were screened for antibacterial activity against various bacterial species by disk diffusion method. Assay was performed using clinical isolates of B. cereus, S. aureus, P. aeruginosa and E. coli. Crude extract of Phyllantus emblica fruit exhibited strong activity against standard cultures of all studied bacteria. Lawsonia alba showed good activity against standard cultures of all the used microorganisms. Coriandrum sativum was effective only against Bacillus cereus, while Cucumis sativus and Culinaris medic showed poor activity against Pseudomonas aeruginosa only. Hence, Phyllantus emblica exhibited strong antibacterial activity against a wide range of bacteria it means that Phyllantus emblica extract contains some compounds which have broad spectrum of bactericidal activity.

  9. Anti-anxiety activity of Coriandrum sativum assessed using different experimental anxiety models.

    Science.gov (United States)

    Mahendra, Poonam; Bisht, Shradha

    2011-09-01

    Interest in alternative medicine and plant-derived medications that affect the "mind" is growing. The aim of present study was to explore the anti-anxiety activity of hydroalcoholic extract of Coriandrum sativum (Linn.) using different animal models (elevated plus maze, open field test, light and dark test and social interaction test) of anxiety in mice. Diazepam (0.5 mg/kg) was used as the standard and dose of hydroalcoholic extract of C. sativum fruit (50, 100 and 200 mg/kg) was selected as per OECD guidelines. Results suggested that extract of C. sativum at 100 and 200 mg/kg dose produced anti-anxiety effects almost similar to diazepam, and at 50 mg/kg dose did not produce anti-anxiety activity on any of the paradigm used. Further studies are needed to identify the anxiolytic mechanism(s) and the phytoconstituents responsible for the observed central effects of the hydroalcoholic extract of C. sativum.

  10. Prevalence of cilantro (Coriandrum sativum) disliking among different ethnocultural groups

    OpenAIRE

    Mauer Lilli; El-Sohemy Ahmed

    2012-01-01

    Abstract Background Cilantro, the leaf of the Coriandrum sativum plant, is an herb that is widely consumed globally and has purported health benefits ranging from antibacterial to anticancer activities. Some individuals report an extreme dislike for cilantro, and this may explain the different cilantro consumption habits between populations. However, the prevalence of cilantro dislike has not previously been reported in any population. The objective of this study was to determine the prevalen...

  11. In vitro efficacy of Coriandrum sativum, Lippia sidoides and Copaifera reticulata against Leishmania chagasi

    OpenAIRE

    Rondon, Fernanda Cristina Macedo; Bevilaqua,Claudia Maria Leal; Accioly,Marina Parissi; Morais,Selene Maia de; Andrade-Júnior,Heitor Franco de; Carvalho, Camila Aparecida de; Lima,Josemar Coelho; Magalhães,Hilton César Rodrigues

    2012-01-01

    The increased incidence of visceral leishmaniasis (VL) in Brazil is due to a lack of effective disease control measures. In addition to that, no effective treatment exists for canine VL in response to synthetic drugs. Thus, the objective of this study was to evaluate the effect of the essential oils of Coriandrum sativum and Lippia sidoides, and oleoresin from Copaifera reticulata, on Leishmania chagasi promastigotes and amastigotes. We also examined the toxicity of these treatments on the mu...

  12. Coriandrum sativum: evaluation of its anxiolytic effect in the elevated plus-maze.

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    Emamghoreishi, Masoumeh; Khasaki, Mohammad; Aazam, Maryam Fath

    2005-01-15

    The clinical applications of benzodiazepines as anxiolytics are limited by their unwanted side effects. Therefore, the development of new pharmacological agents is well justified. Among medicinal plants, Coriandrum sativum L. has been recommended for relief of anxiety and insomnia in Iranian folk medicine. Nevertheless, no pharmacological studies have thus far evaluated its effects on central nervous system. Therefore, the aim of this study was to examine if the aqueous extract of Coriandrum sativum seed has anxiolytic effect in mice. Additionally, its effect on spontaneous activity and neuromuscular coordination were evaluated. The anxiolytic effect of aqueous extract (10, 25, 50, 100 mg/kg, i.p.) was examined in male albino mice using elevated plus-maze as an animal model of anxiety. The effects of the extract on spontaneous activity and neuromuscular coordination were assessed using Animex Activity Meter and rotarod, respectively. In the elevated plus-maze, aqueous extract at 100 mg/kg showed an anxiolytic effect by increasing the time spent on open arms and the percentage of open arm entries, compared to control group. Aqueous extract at 50, 100 and 500 mg/kg significantly reduced spontaneous activity and neuromuscular coordination, compared to control group. These results suggest that the aqueous extract of Coriandrum sativum seed has anxiolytic effect and may have potential sedative and muscle relaxant effects.

  13. In vitro effect of Aloe vera, Coriandrum sativum and Ricinus communis fractions on Leishmania infantum and on murine monocytic cells.

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    Rondon, Fernanda C M; Bevilaqua, Claudia M L; Accioly, Marina P; Morais, Selene M; Andrade-Junior, Heitor F; Machado, Lyeghyna K A; Cardoso, Roselaine P A; Almeida, Camila A; Queiroz-Junior, Eudson M; Rodrigues, Ana Caroline M

    2011-06-10

    In South America, visceral leishmaniasis is a zoonosis caused by the protozoan species Leishmania infantum (syn. L. chagasi) and is primarily transmitted through the bite of the female Lutzomyia longipalpis. Its main reservoir in urban areas is the dog. The application of control measures recommended by health agencies have not achieved significant results in reducing the incidence of human cases, and the lack of effective drugs to treat dogs resulted in the prohibition of this course of action in Brazil. Therefore, it is necessary to search new alternatives for the treatment of canine and human visceral leishmaniasis. The objectives of this study were to evaluate the in vitro effect of fractions from Aloe vera (aloe), Coriandrum sativum (coriander), and Ricinus communis (castor) on promastigotes and amastigotes of L. infantum and to analyze the toxicity against the murine monocytic cells RAW 264.7. To determine the viability of these substances on 50% parasites (IC50), we used a tetrazolium dye (MTT) colorimetric assay (bromide 3-4.5-dimethylthiazol-2-yl-2,5-dephenyltetrazolium), and on amastigotes we performed an in situ ELISA. All fractions were effective against L. infantum promastigotes and did not differ from the positive control pentamidine (p>0.05). However, the R. communis ethyl acetate and chloroform fractions, as well as the C. sativum methanol fraction, were the most effective against amastigotes and did not differ from the positive control amphotericin B (p>0.05). The R. communis ethyl acetate fraction was the least toxic, presenting 83.5% viability of RAW 264.7 cells, which was similar to the results obtained with amphotericin B (p>0.05). Based on these results, we intend to undertake in vivo studies with R. communis ethyl acetate fractions due the high effectiveness against amastigotes and promastigotes of L. infantum and the low cytotoxicity towards murine monocytic cells. Copyright © 2011 Elsevier B.V. All rights reserved.

  14. Alternative treatment of vaginal infections – in vitro antimicrobial and toxic effects of Coriandrum sativum L. and Thymus vulgaris L. essential oils.

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    Bogavac, M; Karaman, M; Janjušević, Lj; Sudji, J; Radovanović, B; Novaković, Z; Simeunović, J; Božin, B

    2015-09-01

    The aims of study were to examine the antibacterial potential of two commercial essential oils (EOs) from coriander (Coriandrum sativum L.) and thyme (Thymus vulgaris L.) against vaginal clinical strains of bacteria and yeast and their chemical composition. Antimicrobial activities of commercial essential oils were determined using macro-diffusion (disc, well) and micro-dilution method in 96-well micro plates against twelve clinical strains of bacteria: Escherichia coli, Proteus mirabilis, Staphylococcus aureus and Enterococcus sp., Staph. aureus ATCC 25923, ATCC 6538 and E. coli 25922 and two clinical Candida albicans strains, including ATTC 10231. Spectrophotometric method was used for determination on C. albicans growth. An antimicrobial effect of EOs was strain specific. Bactericidal activity was higher for coriander EO (minimal inhibitory concentration (MICs) 0·4-45·4 μl ml(-1)) against almost all tested bacteria, except multiple resistant strains of Eneterococcus sp. and Proteus sp. Thyme EO showed slightly better fungicidal activity reaching MIC at 0·11 mg ml(-1) for all C. albicans strains. The effect of EOs on biofilm-forming ability was tested for two strains of Staph. aureus and E. coli, as well as on C. albicans filamentation ability. Brine shrimp lethality bioassay revealed thymus oil total toxicity and coriander oil intoxicity (LC50 = 2·25 mg ml(-1)). The chemical composition of oils was analysed by gas chromatography mass spectrometry showing oxygenated monoterepenes as dominant constituents. The results provide in-vitro scientific support for the safety possible use of Coriander EO against E. coli, Staph. aureus and C. albicans vaginal infections in alternative gynaecological treatment. To examine EOs as possible constituent of naturally based antimicrobial agents in vaginaletes for safety gynaecological application. © 2015 The Society for Applied Microbiology.

  15. Anti-Arrhythmic Potential of Coriandrum sativum Seeds in Salt Induced Arrhythmic Rats

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    Nida Rehman1, Nazish Jahan1*, Khalil-ul-Rahman2, Khalid Mahmood Khan2 and Fatiqa Zafar1

    2016-11-01

    Full Text Available In the present research, the anti-arrhythmic potential of Coriandrum sativum (seeds was evaluated in BaCl2 induced tachycardia and KCl induced bradycardia in rats. Heart rate and electrocardiogram (ECG was recorded during the experimental period. The BaCl2 increased the heart rate from 111/min to 157/min while KCL decreased the heart rate from 112/min to 60/min in the rats of positive control groups. ECG patterns also confirmed the tachy- and brady-arrhythmia in the rats of both positive control groups. The changes in biochemical cardiac biomarkers (CK-MB, LDH, AST, and ALT were also the studied parameters. The level of cardiac biomarkers was significantly elevated in the serum of positive control rats as compared to their respective absolute controls. In case of both curative and preventive mode of treatment the elevated levels of enzymes, cardiac biomarkers were significantly reduced. Electrocardiogram (ECG pattern revealed that the studied plant possesses a very good anti-arrhythmic potential in case of curative mode of treatment. The antiarrhythmic potential through preventive mode of treatment was also encouraging, but comparatively less than the curative mode of treatment. Anti-tachycardial potential of C. sativum was comparable with standard drug while, recovery in bradycardia was relatively slow than standard drug. Gross pathology and ECG pattern of base line group confirmed the innoxious nature of C. sativum seeds. Treatment of rats with Coriandrum sativum (100 mgkg-1 BW normalized the heart rate and attenuated the cardiac arrhythmia.

  16. Coriandrum sativum Suppresses Aβ42-Induced ROS Increases, Glial Cell Proliferation, and ERK Activation.

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    Liu, Quan Feng; Jeong, Haemin; Lee, Jang Ho; Hong, Yoon Ki; Oh, Youngje; Kim, Young-Mi; Suh, Yoon Seok; Bang, Semin; Yun, Hye Sup; Lee, Kyungho; Cho, Sung Man; Lee, Sung Bae; Jeon, Songhee; Chin, Young-Won; Koo, Byung-Soo; Cho, Kyoung Sang

    2016-01-01

    Alzheimer's disease (AD), the most common neurodegenerative disease, has a complex and widespread pathology that is characterized by the accumulation of amyloid [Formula: see text]-peptide (A[Formula: see text]) in the brain and various cellular abnormalities, including increased oxidative damage, an amplified inflammatory response, and altered mitogen-activated protein kinase signaling. Based on the complex etiology of AD, traditional medicinal plants with multiple effective components are alternative treatments for patients with AD. In the present study, we investigated the neuroprotective effects of an ethanol extract of Coriandrum sativum (C. sativum) leaves on A[Formula: see text] cytotoxicity and examined the molecular mechanisms underlying the beneficial effects. Although recent studies have shown the benefits of the inhalation of C. sativum oil in an animal model of AD, the detailed molecular mechanisms by which C. sativum exerts its neuroprotective effects are unclear. Here, we found that treatment with C. sativum extract increased the survival of both A[Formula: see text]-treated mammalian cells and [Formula: see text]42-expressing flies. Moreover, C. sativum extract intake suppressed [Formula: see text]-induced cell death in the larval imaginal disc and brain without affecting A[Formula: see text]42 expression and accumulation. Interestingly, the increases in reactive oxygen species levels and glial cell number in AD model flies were reduced by C. sativum extract intake. Additionally, C. sativum extract inhibited the epidermal growth factor receptor- and A[Formula: see text]-induced phosphorylation of extracellular signal-regulated kinase (ERK). The constitutively active form of ERK abolished the protective function of C. sativum extract against the [Formula: see text]-induced eye defect phenotype in Drosophila. Taken together, these results suggest that C. sativum leaves have antioxidant, anti-inflammatory, and ERK signaling inhibitory properties that

  17. Antibacterial activity of Coriandrum sativum L. and Foeniculum vulgare Miller Var. vulgare (Miller) essential oils.

    Science.gov (United States)

    Lo Cantore, Pietro; Iacobellis, Nicola S; De Marco, Adriana; Capasso, Francesco; Senatore, Felice

    2004-12-29

    Essential oils were extracted from the fruits of Coriandrum sativum L. and Foeniculum vulgare Miller var. vulgare (Miller) and assayed in vitro for antibacterial activity to Escherichia coli and Bacillus megaterium, bacteria routinely used for comparison in the antimicrobial assays, and 27 phytopathogenic bacterial species and two mycopathogenic ones responsible for cultivated mushroom diseases. A significant antibacterial activity, as determined with the agar diffusion method, was shown by C. sativum essential oil whereas a much reduced effect was observed for F. vulgare var. vulgare oil. C. sativum and F. vulgare var. vulgare essential oils may be useful natural bactericides for the control of bacterial diseases of plants and for seed treatment, in particular, in organic agriculture. The significant antibacterial activity of essential oils to the bacterial pathogens of mushrooms appears promising.

  18. Antimicrobial Activity of the Essential Oil from the Leaves and Seeds of Coriandrum sativum toward Food-borne Pathogens.

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    Rezaei, M; Karimi, F; Shariatifar, N; Mohammadpourfard, I; Malekabad, E S

    2015-06-03

    The increasing incidence of drug-resistant pathogens and toxicity of existing antibacterial compounds has drawn attention toward the antimicrobial activity of natural products. The purpose of this study is to evaluate the antimicrobial activity of the essential oil of the leaves and seeds of Coriandrum sativum. The five strains of bacteria comprising Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica, Salmonella enterica and Vibrio cholera were used for the antibacterial tests. In this study, antimicrobial effects of the essential oil from the leaves and seeds of Coriandrum sativum were evaluated by determining the minimum inhibitory concentration (MIC), the inhibition zone and minimum bacteria concentration (MBC). The essential oil from Coriandrum sativum was extracted by steam distillation. The results indicate that the antimicrobial activities against the five pathogens were in the range of 2.5-320 µg/mL. Therefore, an increase in essential oil concentration caused significant increase in inhibitory feature. The essential oil from the leaves and seeds of Coriandrum sativum showed antimicrobial activity against the food-borne pathogenic bacteria. Thus, its oil can be used as an alternative to synthetic food preservative without toxic effects. Also, the oil can be used in biotechnological fields as ingredients in antibiotics and the pharmaceutical industry. These results suggest that the essential oil of C sativum leaves and seeds may have potential use in pharmaceutical and food industries for preservatives or antimicrobial agents.

  19. Coriander Seed Oil Methyl Esters as Biodiesel Fuel: Unique Fatty Acid Composition and Excellent Oxidative Stability

    Science.gov (United States)

    Coriander (Coriandrum sativum L.) seed oil methyl esters were prepared and evaluated as an alternative biodiesel fuel and contained an unusual fatty acid (FA) hitherto unreported as the principle component in biodiesel fuels: petroselinic (6Z-octadecenoic; 68.5 wt %) acid. Most of the remaining FA...

  20. Bioorganic diversity of rare Coriandrum sativum L. honey: unusual chromatographic profiles containing derivatives of linalool/oxygenated methoxybenzene.

    Science.gov (United States)

    Jerković, Igor; Obradović, Marina; Kuś, Piotr Marek; Sarolić, Mladenka

    2013-08-01

    The compounds responsible for highly individual aroma profile of Coriandrum sativum L. honey were isolated by headspace solid-phase microextraction (HS-SPME; used fibers: A: polydimethylsiloxane (PDMS)/divinylbenzene (DVB) and B: divinylbenzene/carboxen/polydimethylsiloxane), as well as ultrasonic solvent extraction (USE; used solvents: A: pentane/Et2 O 1 : 2 (v/v) and B: CH2 Cl2 ) and analyzed by gas chromatography (GC) and mass spectrometry (MS). Unusual chromatographic profiles were obtained containing derivatives of linalool/oxygenated methoxybenzene. trans-Linalool oxide (11.1%; 14.6%) dominated in the headspace, followed by other linalool derivatives (such as cis/trans-anhydrolinalool oxide (5.0%; 5.9%), isomers of lilac aldehyde/alcohol (14.9%; 13.8%) or p-menth-1-en-9-al (15.6%; 18.5%)), octanal, and several low-molecular-weight esters. The major compounds in the solvent extracts were oxygenated methoxybenzene derivatives such as 3,4,5-trimethoxybenzyl alcohol (26.3%; 24.7%), methyl syringate (23.8%; 11.7%), and 3,4-dimethoxybenzyl alcohol (5.6%; 13.9%). Another group of abundant compounds in the extracts were derivatives of linalool (e.g., (E)/(Z)-2,6-dimethylocta-2,7-diene-1,6-diol (17.8%; 16.1%)). Among the compounds identified, cis/trans-anhydrolinalool oxides and 3,4,5-trimethoxybenzyl alcohol can be useful as chemical markers of coriander honey.

  1. Chemical composition of leaf and seed essential oil of Coriandrum sativum L. from Bangladesh

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    Md. Nazrul Islam Bhuiyan, Jaripa Begum and Mahbuba Sultana

    2009-12-01

    Full Text Available The essential oils from leaves and fruits of Coriandrum sativum L. were analyzed by gas chromatography mass spectroscopy (GC-MS. The leaf oil contained 44 compounds mostly of aromatic acids containing 2-decenoic acid (30.8%, E-11-tetradecenoic acid (13.4%, capric acid (12.7%, undecyl alcohol (6.4%, tridecanoic acid (5.5% and undecanoic acid (7.1% as major constituents. The seed oil contains 53 compounds where the major compounds are linalool (37.7%, geranyl acetate (17.6% and γ-terpinene (14.4%. The compositions of both oils varied qualitatively and quantitatively.

  2. Chemical composition of leaf and seed essential oil of Coriandrum sativum L. from Bangladesh

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    Mohammad Nazrul Islam Bhuiyan

    2009-06-01

    Full Text Available The essential oils from leaves and fruits of Coriandrum sativum L. were analyzed by gas chromatography mass spectroscopy (GC-MS. The leaf oil contained 44 compounds mostly of aromatic acids containing 2-decenoic acid (30.8%, E-11-tetradecenoic acid (13.4%, capric acid (12.7%, undecyl alcohol (6.4%, tridecanoic acid (5.5% and undecanoic acid (7.1% as major constituents. The seed oil contains 53 compounds where the major compounds are linalool (37.7%, geranyl acetate (17.6% and γ-terpinene(14.4%. The compositions of both oils varied qualitatively and quantitatively.

  3. Postprandial glycaemia and inhibition of α-glucosidase activity by aqueous extract from Coriandrum sativum.

    Science.gov (United States)

    Brindis, F; González-Andrade, M; González-Trujano, M E; Estrada-Soto, S; Villalobos-Molina, R

    2014-01-01

    The antihyperglycaemic properties of the aqueous extract from the leaves and stems of Coriandrum sativum L. were evaluated in normoglycaemic rats, and on α-glucosidase activity from Saccharomyces cerevisiae, in order to validate its use in folk medicine. In in vivo experiments rats were administered with the aqueous extract of the plant at 100, 300 and 500 mg/kg, to observe the effect on oral sucrose tolerance test. The aqueous extract exhibited significant antihyperglycaemic activity at the three tested doses. In vitro experiments with α-glucosidase exhibited a competitive-type inhibition. These results confirm the antidiabetic properties of the extract of C. sativum L., probably by the inhibition of α-glucosidase in the gastrointestinal tract.

  4. An inhibitive enzyme assay to detect mercury and zinc using protease from Coriandrum sativum.

    Science.gov (United States)

    Baskaran, Gunasekaran; Masdor, Noor Azlina; Syed, Mohd Arif; Shukor, Mohd Yunus

    2013-01-01

    Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this study, an inhibitive enzyme assay for heavy metals has been developed using crude proteases from Coriandrum sativum. In this assay, casein was used as a substrate and Coomassie dye was used to denote the completion of casein hydrolysis. In the absence of inhibitors, casein was hydrolysed and the solution became brown, while in the presence of metal ions such as Hg²⁺ and Zn²⁺, the hydrolysis of casein was inhibited and the solution remained blue. Both Hg²⁺ and Zn²⁺ exhibited one-phase binding curve with IC₅₀ values of 3.217 mg/L and 0.727 mg/L, respectively. The limits of detection (LOD) and limits of quantitation (LOQ) for Hg were 0.241 and 0.802 mg/L, respectively, while the LOD and LOQ for Zn were 0.228 and 0.761 mg/L, respectively. The enzyme exhibited broad pH ranges for activity. The crude proteases extracted from Coriandrum sativum showed good potential for the development of a rapid, sensitive, and economic inhibitive assay for the biomonitoring of Hg²⁺ and Zn²⁺ in the aquatic environments.

  5. Effects of Intercropping on Biological Yield, Percentage of Nitrogen and Morphological Characteristics of Coriander and Fenugreek

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    R. Bigonah

    2014-12-01

    Full Text Available In order to study the intercropping arrangements of coriander (Coriandrum sativum L. and fenugreek (Trigonella foenum-graecum L., a field experiment was conducted during growing season of 2010 at Agriculture Research Station, College of Agriculture, Ferdowsi University of Mashhad, Iran. A randomized complete block design with three replications was used. Treatments included: mono-crop of fenugreek (A, %25 of optimum density of coriander + %175 of optimum density of fenugreek (B, %50 of optimum density of coriander + %150 of optimum density of fenugreek (C, %75 of optimum density of coriander + %125 of optimum density of fenugreek (D, %100 of optimum density of coriander + %100 of optimum density of fenugreek (E, mono-crop of coriander (F, %125 of optimum density of coriander + %75 of optimum density of fenugreek (G, %150 of optimum density of coriander + %50 of optimum density of fenugreek (H, %175 of optimum density of coriander + %25 of optimum density of fenugreek (I. Biological yield harvested in coriander at %5 flowering stage and in fenugreek at %20 flowering stage. The result showed that B treatment had highest plant height and biological yield of fenugreek, highest total land equivalent ratio and also B treatment had lowest essential oil contents of leaf, essential oil yield and biological yield of coriander. I treatment had lowest biological yield of fenugreek and it had highest essential oil contents of leaf, essential oil yield and plant height in coriander. Also A and E treatments had highest percent of nitrogen of biomass in fenugreek and coriander, respectively.

  6. Preventive effect of Coriandrum sativum on neuronal damages in pentylentetrazole-induced seizure in rats

    Science.gov (United States)

    Pourzaki, Mojtaba; Homayoun, Mansour; Sadeghi, Saeed; Seghatoleslam, Masoumeh; Hosseini, Mahmoud; Ebrahimzadeh Bideskan, Alireza

    2017-01-01

    Objective: Coriandrum sativum (C. sativum) as a medicinal plant has been pointed to have analgesic, hypnotic and anti-oxidant effects. In the current study, a possible preventive effect of the hydro-alcoholic extract of the plant on neuronal damages was examined in pentylenetetrazole (PTZ) rat model of seizure. Materials and Methods: Forty male rats were divided into five main groups and treated by (1) saline, (2) PTZ: 100 mg/kg PTZ (i.p) and (3-5) 50, 100 and 200 mg/kg of hydro-alcoholic extract of C. sativum during seven consecutive days before PTZ injection. After electrocorticography (ECoG), the brains were removed to use for histological examination. Results: All doses of the extract reduced duration, frequency and amplitude of the burst discharges while prolonged the latency of the seizure attacks (p<0.05, p<0.01, and p<0.001). Administration of all 3 doses of the extract significantly prevented from production of dark neurons (p<0.01, and p<0.001) and apoptotic cells (p<0.05, p<0.01, and p<0.001) in different areas of the hippocampus compared to PTZ group. Conclusion: The results of this study allow us to conclude that C. sativum, because of its antioxidant properties, prevents from neuronal damages in PTZ rat model of seizure.

  7. Anti-anxiety activity of Coriandrum sativum assessed using different experimental anxiety models

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    Poonam Mahendra

    2011-01-01

    Full Text Available Interest in alternative medicine and plant-derived medications that affect the "mind" is growing. The aim of present study was to explore the anti-anxiety activity of hydroalcoholic extract of Coriandrum sativum (Linn. using different animal models (elevated plus maze, open field test, light and dark test and social interaction test of anxiety in mice. Diazepam (0.5 mg/kg was used as the standard and dose of hydroalcoholic extract of C. sativum fruit (50, 100 and 200 mg/kg was selected as per OECD guidelines. Results suggested that extract of C. sativum at 100 and 200 mg/kg dose produced anti-anxiety effects almost similar to diazepam, and at 50 mg/kg dose did not produce anti-anxiety activity on any of the paradigm used. Further studies are needed to identify the anxiolytic mechanism(s and the phytoconstituents responsible for the observed central effects of the hydroalcoholic extract of C. sativum.

  8. Action of Coriandrum sativum L. Essential Oil upon Oral Candida albicans Biofilm Formation

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    V. F. Furletti

    2011-01-01

    Full Text Available The efficacy of extracts and essential oils from Allium tuberosum, Coriandrum sativum, Cymbopogon martini, Cymbopogon winterianus, and Santolina chamaecyparissus was evaluated against Candida spp. isolates from the oral cavity of patients with periodontal disease. The most active oil was fractionated and tested against C. albicans biofilm formation. The oils were obtained by water-distillation and the extracts were prepared with macerated dried plant material. The Minimal Inhibitory Concentration—MIC was determined by the microdilution method. Chemical characterization of oil constituents was performed using Gas Chromatography and Mass Spectrometry (GC-MS. C. sativum activity oil upon cell and biofilm morphology was evaluated by Scanning Electron Microscopy (SEM. The best activities against planktonic Candida spp. were observed for the essential oil and the grouped F8–10 fractions from C. sativum. The crude oil also affected the biofilm formation in C. albicans causing a decrease in the biofilm growth. Chemical analysis of the F8–10 fractions detected as major active compounds, 2-hexen-1-ol, 3-hexen-1-ol and cyclodecane. Standards of these compounds tested grouped provided a stronger activity than the oil suggesting a synergistic action from the major oil constituents. The activity of C. sativum oil demonstrates its potential for a new natural antifungal formulation.

  9. [Isolation and identification of flavon(ol)-O-glycosides in caraway (Carum carvi L.), fennel (Foeniculum vulgare Mill.), anise (Pimpinella anisum L.), and coriander (Coriandrum sativum L.), and of flavon-C-glycosides in anise. I. Phenolics of spices (author's transl)].

    Science.gov (United States)

    Kunzemann, J; Herrmann, K

    1977-07-29

    The flavonoid constituents of various spices were separated by means of chromatography on cellulose colums, and the following compounds were obtained crystalline: Quercetin 3-glucuronide from caraway, fennel, anise, and coriander; isoquercitrin from caraway and fennel; rutin from fennel and anise; quercetin 3-O-caffeylglucoside and kaempferol 3-glucoside from caraway; quercetin 3-arabinoside from fennel, and luteolin 7-glucoside, isoorientin and isovitexin from anise. Other constitutents which were however not obtained crystalline, but which could be identified by the usual procedures were kaempferol 3-glucuronide and kaempferol 3-arabinoside in fennel, apigenin 7-glucoside and a luteolin glycoside in anise, and isoquercitrin and rutin in coriander. The glycosides contained in the fruit of the four spices also occur in the leaves. Leaves of caraway and fennel in addition contain isorhammetin glycosides in low concentration.

  10. Coriandrum sativum and Lavandula angustifolia Essential Oils: Chemical Composition and Activity on Central Nervous System.

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    Caputo, Lucia; Souza, Lucéia Fátima; Alloisio, Susanna; Cornara, Laura; De Feo, Vincenzo

    2016-11-30

    The aims of this study are to determine the chemical composition of Lavandula angustifolia Mill. and Coriandrum sativum L. essential oils, to evaluate their cytotoxic effects in SH-SY5Y human neuroblastoma cells, to investigate whether an alteration of adenylate cyclase 1 (ADCY1) and of extracellular signal-regulated kinase (ERK) expression can take part in the molecular mechanisms of the essential oils, and to study their possible neuronal electrophysiological effects. The essential oils were obtained by hydrodistillation, and studied by GC and GC-MS. In the oils from L. angustifolia and C. sativum, linalool was the main component (33.1% and 67.8%, respectively). SH-SY5Y cells were incubated with different concentrations of essential oils and of linalool. Cell viability and effects on ADCY1 and ERK expression were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT and Western blotting, respectively. Variation in cellular electrophysiology was studied in primary cultures of rat cortical neurons with a multi-electrode array (MEA)-based approach. The essential oils and linalool revealed different cytotoxic activities. Linalool inhibited ADCY1 and ERK expression. Neuronal networks subjected to L. angustifolia and C. sativum essential oils showed a concentration-dependent inhibition of spontaneous electrical activity.

  11. Hypnotic effect of Coriandrum sativum, Ziziphus jujuba, Lavandula angustifolia and Melissa officinalis extracts in mice.

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    Hajhashemi, Valiollah; Safaei, Azadeh

    2015-01-01

    The aim of the present study was to evaluate hypnotic effect of Coriandrum sativum, Ziziphus jujuba, Lavandula angustifolia and Melissa officinalis hydroalcoholic extracts in mice to select the most effective ones for a combination formula. Three doses of the extracts (250, 500 and 1000 mg/kg of C. sativum and Z. jujuba and 200, 400 and 800 mg/kg of L. angustifolia and M. officinalis) were orally administered to male Swiss mice (20-25 g) and one hour later pentobarbital (50 mg/kg, i.p.) was injected to induce sleep. Onset of sleep and its duration were measured and compared. Control animals and reference group received vehicle (10 ml/kg, p.o.) and diazepam (3 mg/kg, i.p.), respectively. C. sativum and Z. jujuba failed to change sleep parameters. L. angustifolia at doses of 200, 400 and 800 mg/kg shortened sleep onset by 7.6%, 50% and 51.5% and prolonged sleep duration by 9.9%, 43.1% and 80.2%, respectively. Compared with control group the same doses of M. officinalis also decreased sleep onset by 24.7%, 27.5% and 51.2% and prolonged sleep duration by 37.9%, 68.7% and 131.7% respectively. Combinations of L. angustifolia and M. officinalis extracts showed additive effect and it is suggested that a preparation containing both extracts may be useful for insomnia.

  12. Analysis of the essential oils of Coriandrum sativum Using GC-MS coupled with chemometric resolution methods.

    Science.gov (United States)

    Zhou, Zhi-Feng; Chen, Ling-Yun; Shen, Mei; Ma, An-De; Yang, Xue-Mei; Zou, Fei

    2011-01-01

    The essential oils extracted from Coriandrum sativum L. were analyzed by GC-MS coupled with chemometric resolution methods. Through the chemometric resolution methods, peak clusters were uniquely resolved into the pure chromatographic profiles and mass spectra of each component. Qualitative analysis was performed by comparing the pure mass spectra with those in the NIST 05 mass spectral library. Quantitative analysis was performed using the total volume integration method. A total of 118 constituents were detected, of which 104 were identified, accounting for 97.27% of the total content. The results indicate that GC-MS combined with chemometric resolution methods can greatly enhance the capability of separation and the reliability of qualitative and quantitative results. The combined method is an economical and accurate approach for the rapid analysis of the complex essential oil samples in Coriandrum sativum L.

  13. Evaluation of bioactivity of linalool-rich essential oils from Ocimum basilucum and Coriandrum sativum varieties.

    Science.gov (United States)

    Duman, Ahmet D; Telci, Isa; Dayisoylu, Kenan S; Digrak, Metin; Demirtas, Ibrahim; Alma, Mehmet H

    2010-06-01

    Essential oils from Ocimum basilicum L. and Coriandrum sativum L. varieties originating from Turkey were investigated for their antimicrobial properties. The antimicrobial effects of the oil varieties were evaluated by the disc diffusion and minimum inhibitory concentration (MIC) methods against eight bacteria and three fungi. The compositions of the essential oils were analyzed and identified by GC and GC-MS. O. basilicum, C. sativum var. macrocarpum and var. microcarpum oils revealed the presence of linalool (54.4%), eugenol (9.6%), methyl eugenol (7.6%); linalool (78.8%), gamma-terpinene (6.0%), nerol acetate (3.5%); and linalool (90.6%), and nerol acetate (3.3%) as the major components, respectively. The oils exhibited antibacterial activity ranging from 1.25 to 10 microL disc(-1) against the test organisms with inhibition zones of 9.5-39.0 mm and minimal inhibitory concentrations values in the range 0.5- > or =1 microL/L. Linalool, eugenol, and methyl eugenol at 1.25 microL disc(-1) had antimicrobial effects on all microorganisms, giving inhibition zones ranging from 7 to 19 mm.

  14. Protective effects of Coriandrum sativum extracts on carbon tetrachloride-induced hepatotoxicity in rats.

    Science.gov (United States)

    Sreelatha, S; Padma, P R; Umadevi, M

    2009-04-01

    Oxidative damage is implicated in the pathogenesis of various liver injuries. The study was aimed to investigate the antioxidant activity of Coriandrum sativum on CCl(4) treated oxidative stress in Wistar albino rats. CCl(4) injection induced oxidative stress by a significant rise in serum marker enzymes and thiobarbituric acid reactive substances (TBARS) along with the reduction of antioxidant enzymes. In serum, the activities of enzymes like ALP, ACP and protein and bilirubin were evaluated. Pretreatment of rats with different doses of plant extract (100 and 200mg/kg) significantly lowered SGOT, SGPT and TBARS levels against CCl(4) treated rats. Hepatic enzymes like SOD, CAT, GPx were significantly increased by treatment with plant extract, against CCl(4) treated rats. Histopathological examinations showed extensive liver injuries, characterized by extensive hepatocellular degeneration/necrosis, inflammatory cell infiltration, congestion, and sinusoidal dilatation. Oral administration of the leaf extract at a dose of 200mg/kg body weight significantly reduced the toxic effects of CCl(4). The activity of leaf extract at the dose of 200mg/kg was comparable to the standard drug, silymarin. Based on these results, it was observed that C. sativum extract protects liver from oxidative stress induced by CCl(4) and thus helps in evaluation of traditional claim on this plant.

  15. Composition of the essential oil constituents from leaves and stems of Korean Coriandrum sativum and their immunotoxicity activity on the Aedes aegypti L.

    Science.gov (United States)

    Chung, Ill-Min; Ahmad, Ateeque; Kim, Sun-Jin; Naik, Poornanand Madhava; Nagella, Praveen

    2012-02-01

    The leaves and stems of Coriandrum sativum were extracted and the essential oil composition and immunotoxicity effects were studied. The analyses were conducted by gas chromatography-mass spectroscopy (GC-MS), which revealed the essential oils of C. sativum leaves and stems. Thirty-nine components representing 99.62% of the total oil were identified from the leaves. The major components are cyclododecanol (23.11%), tetradecanal (17.86%), 2-dodecenal (9.93%), 1-decanol (7.24%), 13-tetradecenal (6.85%), 1-dodecanol (6.54%), dodecanal (5.16%), 1-undecanol (2.28%), and decanal (2.33%). Thirty-eight components representing 98.46% of the total oil were identified from the stems of the coriander. The major components are phytol (61.86%), 15-methyltricyclo[6.5.2(13,14),0(7,15)]-pentadeca-1,3,5,7,9,11,13-heptene (7.01%), dodecanal (3.18%), and 1-dodecanol (2.47%). The leaf oil had significant toxic effects against the larvae of Aedes aegypti with an LC₅₀ value of 26.93 ppm and an LC₉₀ value of 37.69 ppm and the stem oil has toxic effects against the larvae of A. aegypti with an LC₅₀ value of 29.39 ppm and an LC₉₀ value of 39.95 ppm. Also, the above data indicate that the major compounds may play an important role in the toxicity of essential oils.

  16. The effects of different fractions of Coriandrum sativum on pentylenetetrazole-induced seizures and brain tissues oxidative damage in rats

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    Akbar Anaeigoudari

    2016-03-01

    Full Text Available Objective: In the present work, the effects of different fractions of Coriandrum sativum (C. sativum, on pentylenetetrazole (PTZ-induced seizures and brain tissues oxidative damage were investigated in rats. Materials and Methods: The rats were divided into the following groups: (1 vehicle, (2 PTZ (90 mg/kg, (3 water fraction (WF of C. sativum (25 and 100 mg/kg, (4 n-butanol fraction (NBF of C. sativum (25 and 100 mg/kg, and (5 ethyl acetate fraction (EAF of C. sativum (25 and 100 mg/kg. Results: The first generalized tonic-clonic seizures (GTCS latency in groups treated with 100 mg /kg of WF or EAF was significantly higher than that of PTZ group (p< 0.01. In contrast to WF, the EAF and NBF were not effective in increasing the first minimal clonic seizure (MCS latency. Malondialdehyde (MDA levels in both cortical and hippocampal tissues of PTZ group were significantly higher than those of control animals (p< 0.001. Pretreatment with WF, NBF, or EAF resulted in a significant reduction in the MDA levels of hippocampi (pConclusion: The present study showed that different fractions of C. sativum possess antioxidant activity in the brain and WF and EAF of this plant have anticonvulsant effects.

  17. Antioxidant activity of essential oil from Coriandrum Sativum L. in Italian salami

    OpenAIRE

    2011-01-01

    Four formulations of Italian salami type were produced: without antioxidants; with essential oil of coriander essential oil (0.01%); with BHT (0.01%); and with Coriander essential oil and BHT (0.005 and 0.005%). The antioxidant activity of salamis was evaluated by the lipid oxidation, through the techniques of peroxide number and TBARS. The salami with the coriander essential oil exhibited reduction in lipid oxidation by increasing the shelf life of the product. The salami with the coriander ...

  18. In vitro efficacy of Coriandrum sativum, Lippia sidoides and Copaifera reticulata against Leishmania chagasi.

    Science.gov (United States)

    Rondon, Fernanda Cristina Macedo; Bevilaqua, Claudia Maria Leal; Accioly, Marina Parissi; de Morais, Selene Maia; de Andrade-Júnior, Heitor Franco; de Carvalho, Camila Aparecida; Lima, Josemar Coelho; Magalhães, Hilton César Rodrigues

    2012-01-01

    The increased incidence of visceral leishmaniasis (VL) in Brazil is due to a lack of effective disease control measures. In addition to that, no effective treatment exists for canine VL in response to synthetic drugs. Thus, the objective of this study was to evaluate the effect of the essential oils of Coriandrum sativum and Lippia sidoides, and oleoresin from Copaifera reticulata, on Leishmania chagasi promastigotes and amastigotes. We also examined the toxicity of these treatments on the murine monocyte cell line RAW 264.7. To determine the IC50 a MTT test (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was performed on promastigotes, and an in situ ELISA assay was conducted on amastigotes. Here, we demonstrate that oleoresin from C. reticulata was effective against both promastigotes (IC50 of 7.88 µg.mL-1) and amastigotes (IC50 of 0.52 µg.mL-1), and neither of the two treatments differed significantly (p > 0.05) from pentamidine (IC50 of 2.149 µg.mL-1) and amphotericin B (IC50 of 9.754 µg.mL-1). Of the three plant oils tested, only oleoresin showed no toxicity toward monocyte, with 78.45% viability after treatment. Inhibition of promastigote and amastigote growth and the lack of cytotoxicity by C. reticulata demonstrate that oleoresin may be a viable option for analyzing the in vivo therapeutic effects of leishmanicidal plants.

  19. Toxicity assessment of cerium oxide nanoparticles in cilantro (Coriandrum sativum L.) plants grown in organic soil.

    Science.gov (United States)

    Morales, Maria Isabel; Rico, Cyren M; Hernandez-Viezcas, Jose Angel; Nunez, Jose E; Barrios, Ana Cecilia; Tafoya, Alejandro; Flores-Marges, Juan Pedro; Peralta-Videa, Jose R; Gardea-Torresdey, Jorge L

    2013-07-03

    Studies have shown that CeO₂ nanoparticles (NPs) can be accumulated in plants without modification, which could pose a threat for human health. In this research, cilantro (Coriandrum sativum L.) plants were germinated and grown for 30 days in soil amended with 0 to 500 mg kg⁻¹ CeO₂ NPs and analyzed by spectroscopic techniques and biochemical assays. At 125 mg kg⁻¹, plants produced longer roots (p ≤ 0.05), and at 500 mg kg⁻¹, there was higher Ce accumulation in tissues (p ≤ 0.05). At 125 mg, catalase activity significantly increased in shoots and ascorbate peroxidase in roots (p ≤ 0.05). The FTIR analyses revealed that at 125 mg kg⁻¹ the CeO₂ NPs changed the chemical environment of carbohydrates in cilantro shoots, for which changes in the area of the stretching frequencies were observed. This suggests that the CeO₂ NPs could change the nutritional properties of cilantro.

  20. Antioxidant activity of essential oil from Coriandrum Sativum L. in Italian salami

    Directory of Open Access Journals (Sweden)

    Cristiane Marangoni

    2011-03-01

    Full Text Available Four formulations of Italian salami type were produced: without antioxidants; with essential oil of coriander essential oil (0.01%; with BHT (0.01%; and with Coriander essential oil and BHT (0.005 and 0.005%. The antioxidant activity of salamis was evaluated by the lipid oxidation, through the techniques of peroxide number and TBARS. The salami with the coriander essential oil exhibited reduction in lipid oxidation by increasing the shelf life of the product. The salami with the coriander essential oil and BHT showed no synergism between the antioxidants. The salami using BHT presented less antioxidant activity than that of the salami using coriander essential oil.

  1. Therapeutic efficacies of Coriandrum sativum aqueous extract against metronidazole-induced genotoxicity in Channa punctatus peripheral erythrocytes.

    Science.gov (United States)

    Talapatra, Soumendra Nath; Dasgupta, Subham; Guha, Gunjan; Auddy, Moumita; Mukhopadhyay, Aniruddha

    2010-12-01

    Metronidazole (MTZ), a nitroimidazole drug, is primarily used as an anti-protozoan or an anti-bacterial agent in humans, although its genotoxic and carcinogenic effects have been widely reported, particularly in aquatic organisms. MTZ may induce DNA damages through single-strand breaks, modification of bases, DNA-DNA and DNA-protein cross-links, ultimately leading to apoptosis or necrosis. Here, we have assessed the genotoxicity of MTZ in the peripheral erythrocytes of Channa punctatus, using micronucleation (MN) and binucleation (BN) as genotoxicity markers. The therapeutic potential of aqueous extract of Coriandrum sativum against MTZ-induced genotoxicity has also been examined. The results show significant (Psativum leaf extract. Hence, we establish that MTZ can produce considerable degrees of micronucleus and binucleus formation in peripheral erythrocytes of C. punctatus, and such deleterious effect of MTZ treatment can be mitigated by aqueous extract of C. sativum leaves.

  2. Opiate System Mediate the Antinociceptive Effects of Coriandrum sativum in Mice.

    Science.gov (United States)

    Taherian, Abbas Ali; Vafaei, Abbas Ali; Ameri, Javad

    2012-01-01

    Our previous study showed that Coriandrum sativum (CS) has antinociceptive effects, but the mechanisms that mediate this effect are not clear. The present study was designed to test the role of opiate system in the antinociceptive effects of CS on acute and chronic pain in mice using Hot Plate (HP), Tail Flick (TF) and Formalin (FT) tests and also to compare its effect with dexamethasone (DEX) and stress (ST). Young adult male albino mice (25-30 g) in 33 groups (n = 8 in each group) were used in this study. CS (125 250, 500 and 1000 mg/Kg IP), DEX (0.5, 1 and 2 mg/Kg IP), vehicle (VEH) or swim stress were used 30 min before the pain evaluation tests. Acute and chronic pain was assessed by HP, TF and FT models. In addition, Naloxone (NAL, 2 mg/Kg, IP) was injected 15 min before the CS extract administration in order to assess the role of opiate system in the antinociception of CS. Results indicated that CS, DEX and ST have analgesic effects (p < 0.01) in comparison with the control group and higher dose of CS was more effective (p < 0.001). Besides, pretreatment of NAL modulates the antinociceptive effects of CS in all models (p < 0.001). The above findings showed that CS, DEX and ST have modulator effects on pain. These findings further indicate that the CS extract has more analgesic effects than DEX and ST and also provides the evidence for the existence of an interaction between antinociceptive effects of CS and opiate system.

  3. Copper nanoparticles/compounds impact agronomic and physiological parameters in cilantro (Coriandrum sativum).

    Science.gov (United States)

    Zuverza-Mena, Nubia; Medina-Velo, Illya A; Barrios, Ana C; Tan, Wenjuan; Peralta-Videa, Jose R; Gardea-Torresdey, Jorge L

    2015-10-01

    The environmental impacts of Cu-based nanoparticles (NPs) are not well understood. In this study, cilantro (Coriandrum sativum) was germinated and grown in commercial potting mix soil amended with Cu(OH)2 (Kocide and CuPRO), nano-copper (nCu), micro-copper (μCu), nano-copper oxide (nCuO), micro-copper oxide (μCuO) and ionic Cu (CuCl2) at either 20 or 80 mg Cu per kg. In addition to seed germination and plant elongation, relative chlorophyll content and micro and macroelement concentrations were determined. At both concentrations, only nCuO, μCuO, and ionic Cu, showed statistically significant reductions in germination. Although compared with control, the relative germination was reduced by ∼50% with nCuO at both concentrations, and by ∼40% with μCuO, also at both concentrations, the difference among compounds was not statistically significant. Exposure to μCuO at both concentrations and nCu at 80 mg kg(-1) significantly reduced (p≤ 0.05) shoot elongation by 11% and 12.4%, respectively, compared with control. Only μCuO at 20 mg kg(-1) significantly reduced (26%) the relative chlorophyll content, compared with control. None of the treatments increased root Cu, but all of them, except μCuO at 20 mg kg(-1), significantly increased shoot Cu (p≤ 0.05). Micro and macro elements B, Zn, Mn, Ca, Mg, P, and S were significantly reduced in shoots (p≤ 0.05). Similar results were observed in roots. These results showed that Cu-based NPs/compounds depress nutrient element accumulation in cilantro, which could impact human nutrition.

  4. In vitro effects of Coriandrum sativum, Tagetes minuta, Alpinia zerumbet and Lantana camara essential oils on Haemonchus contortus.

    Science.gov (United States)

    Macedo, Iara Tersia Freitas; de Oliveira, Lorena Mayana Beserra; Camurça-Vasconcelos, Ana Lourdes Fernandes; Ribeiro, Wesley Lyeverton Correia; dos Santos, Jessica Maria Leite; de Morais, Selene Maia; de Paula, Haroldo Cesar Beserra; Bevilaqua, Claudia Maria Leal

    2013-01-01

    Phytotherapy can be an alternative for the control of gastrointestinal parasites of small ruminants. This study evaluated the efficacy of Alpinia zerumbet, Coriandrum sativum, Tagetes minuta and Lantana camara essential oils by two in vitro assays on Haemonchus contortus, an egg hatch test (EHT) and larval development test (LDT). No effect was observed for L. camara in the EHT. A. zerumbet, C. sativum and T. minuta essential oils exhibited a dose-dependent effect in the EHT, inhibiting 81.2, 99 and 98.1% of H. contortus larvae hatching, respectively, at a concentration of 2.5 mg mL-1. The effective concentration to inhibit 50% (EC50) of egg hatching was 0.94, 0.63 and 0.53 mg mL-1 for A. zerumbet, C. sativum and T. minuta essential oils, respectively. In LDT, L. camara, A. zerumbet, C. sativum and T. minuta at concentration of 10 mg mL-1 inhibited 54.9, 94.2, 97.8 and 99.5% of H. contortus larval development, presenting EC50 values of 6.32, 3.88, 2.89 and 1.67 mg mL-1, respectively. Based on the promising results presented in this in vitro model, it may be possible use of these essential oils to control gastrointestinal nematodes. However, their anthelmintic activity should be confirmed in vivo.

  5. In vitro effects of Coriandrum sativum, Tagetes minuta, Alpinia zerumbet and Lantana camara essential oils on Haemonchus contortus

    Directory of Open Access Journals (Sweden)

    Iara Tersia Freitas Macedo

    Full Text Available Phytotherapy can be an alternative for the control of gastrointestinal parasites of small ruminants. This study evaluated the efficacy of Alpinia zerumbet, Coriandrum sativum, Tagetes minuta and Lantana camaraessential oils by two in vitro assays on Haemonchus contortus, an egg hatch test (EHT and larval development test (LDT. No effect was observed for L. camara in the EHT. A. zerumbet, C. sativum and T. minuta essential oils exhibited a dose-dependent effect in the EHT, inhibiting 81.2, 99 and 98.1% of H. contortus larvae hatching, respectively, at a concentration of 2.5 mg mL-1. The effective concentration to inhibit 50% (EC50 of egg hatching was 0.94, 0.63 and 0.53 mg mL-1 for A. zerumbet, C. sativum and T. minuta essential oils, respectively. In LDT, L. camara, A. zerumbet, C. sativum and T. minuta at concentration of 10 mg mL-1 inhibited 54.9, 94.2, 97.8 and 99.5% of H. contortus larval development, presenting EC50 values of 6.32, 3.88, 2.89 and 1.67 mg mL-1, respectively. Based on the promising results presented in this in vitro model, it may be possible use of these essential oils to control gastrointestinal nematodes. However, their anthelmintic activity should be confirmed in vivo.

  6. Synergistic activity of coriander oil and conventional antibiotics against Acinetobacter baumannii.

    Science.gov (United States)

    Duarte, A; Ferreira, S; Silva, F; Domingues, F C

    2012-02-15

    In this study we investigated the existence of synergistic antibacterial effect between coriander (Coriandrum sativum L.) essential oil and six different antibacterial drugs (cefoperazone, chloramphenicol, ciprofloxacin, gentamicin, tetracycline and piperacillin). The antibacterial activity of coriander oil was assessed using microdilution susceptibility testing and synergistic interaction by checkerboard assays. The association of coriander essential oil with chloramphenicol, ciprofloxacin, gentamicin and tetracycline against Acinetobacter baumannii showed in vitro effectiveness, which is an indicator of a possible synergistic interaction against two reference strains of A. baumannii (LMG 1025 and LMG 1041) (FIC index from 0.047 to 0.375). However, when tested the involvement between coriander essential oil and piperacillin or cefoperazone, the isobolograms and FIC index showed an additive interaction. The in vitro interaction could improve the antimicrobial effectiveness of ciprofloxacin, gentamicin and tetracycline and may contribute to resensitize A. baumannii to the action of chloramphenicol.

  7. Sensory profile of Italian salami with coriander (Coriandrum sativum L.) essential oil

    OpenAIRE

    Cristiane Marangoni; Neusa Fernandes de Moura

    2011-01-01

    The descriptive terminology and sensory prolife of four samples of Italian salami were determined using a methodology based on the Quantitative Descriptive Analysis (QDA). A sensory panel consensually defined sensory descriptors, their respective reference materials, and the descriptive evaluation ballot. Twelve individuals were selected as judges and properly trained. They used the following criteria: discriminating power, reproducibility, and individual consensus. Twelve descriptors were de...

  8. Biofenologia do coentro Biophenology of coriander

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    José Higino Ribeiro dos Santos

    1992-07-01

    Full Text Available Estudou-se a biofenologia do coentro, Coriandrum sativum L., com a finalidade de obter-se informações dos aspectos relativos à fase vegetativa e reprodutiva da cultura, corno também o comportamento desta olerícola em relação às diferentes épocas de plantio. Concluiu-se que, para as condições do Estado do Ceará, onde as temperaturas são mais baixas próximo ao meio do ano e altas no final e início do ano, o fator que mais influencia a biofenologia do coentro, nesta região, é a temperatura.Phenology of the reprodutive and vegetative growth of coriander,Coriandrum sativum L., was studied as well as the behaviour of that vegetable in relation to different times of sowing. It was concluded, for the conditions of Ceará State, Brazil, which presents lower temperature values near the middle of the year and high her temperature values in the final and beginning the year, that the factor temperature is the one that most influences phenology of coriander.

  9. Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica

    Directory of Open Access Journals (Sweden)

    Sanghamitra Dutta

    2016-04-01

    Full Text Available Antioxidants are naturally occurring substances that combat oxidative damage in biological entities. An antioxidant achieves this by slowing or preventing the oxidation process that can damage cells in the body. It does this by getting oxidized itself in place of the cells. The aim of the present study was to evaluate the in vitro antioxidant and free radical scavenging activities of aqueous and 95% methanol leaf extracts of four herbs viz. Trigonella foenum-graecum L, Murraya koenigii, Coriandrum sativum and Centella asiatica which have frequent use in Indian cuisine. Both aqueous and 95% methanol leaf extracts have shown significant amount reducing power. Both aqueous and 95% methanol leaf extracts of Coriandrum sativum had significant DPPH radical scavenging activity with IC50 value of 0.21± 0.3 mg/L and 0.176 ± 0.008 mg/L respectively. The aqueous leaf extract of Trigonella foenum-graecum L showed low scavenging activity. Among all the leaf extracts, the aqueous leaf extract of Centella asiatica has exhibited significantly high NO radical scavenging activity (80% with IC50 value of 0.11 ± 0.17 mg/L. The aqueous leaf extracts of the samples have showed significantly high superoxide radical scavenging activity. The activity was maximum for the aqueous leaf extract of Centella asiatica, IC50 value is 4.36 ± 0.41 mg/L. anti lipid peroxide activities were very high ( 90 % for aqueous leaf extracts of Coriandrum sativum (IC50 = 0.064 ± 0.85 mg/L and Centella asiatica (IC50 = 0.066 ± 0.9mg/L at a concentration of 0.16 mg/L. The aqueous leaf extracts of the samples were found to contain large amounts of flavonoids and phenolic compounds and exhibited high antioxidant and free radical scavenging activities. These in vitro assays indicate that these plant extracts are significant source of natural antioxidants which might be helpful in preventing the progress of various oxidative stresses.

  10. Effect of coriander on thioacetamide-induced hepatotoxicity in rats.

    Science.gov (United States)

    Moustafa, Abdel Halim A; Ali, Ehab Mostafa M; Moselhey, Said S; Tousson, Ehab; El-Said, Karim S

    2014-08-01

    Thioacetamide (TAA) is a potent hepatotoxin that causes centrilobulal necrosis and nephrotoxic damage following acute administration. Prolonged exposure to TAA can result in bile duct proliferation and liver cirrhosis histologically similar to that caused due to viral hepatitis infection. Coriander in food increases the antioxidant content, acting as a natural antioxidant and inhibiting undesirable oxidation processes. The present study investigated the antioxidant activity of Coriandrum sativum on TAA-induced hepatotoxicity in the male rats. Phenolic content and antioxidant activity were evaluated in the coriander leaves and seeds. Forty-eight adult male rats were divided into four groups. Group I (control), group II (TAA injected rats), group III (TAA injected rats fed coriander leaves) and group IV (TAA injected rats fed coriander seeds). The results revealed that serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities were significantly increased in the groups II, III and IV as compared to the normal control. Oxidative stress in the group II was manifested by a significant rise in nitric oxide (NO), thiobarbituric acid reactive substance (TBARS) levels and myloperoxidase (MPO) activities in the liver tissues as compared with the control group. Rats fed with coriander leaves and seeds showed a decrease in the serum ALT, AST and ALP activities and in the liver NO and TBARS levels as compared to the group II. Histopathological study revealed that coriander feeding attenuated TAA-induced hepatotoxicity in the rats. In conclusion, coriander leaves attenuate hepatotoxicity induced by TAA more than that of seeds due to the higher content of phenolic compounds and antioxidants in the leaves of coriander. Liver of rats intoxicated with TAA exhibited advanced CIRRHOSIS: in the form of macronodular and micronodular structure surrounded by fibrous tissue. Treatment with coriander leaves and seeds helps in improving

  11. Studies on gamma irradiated medicinal plants and spices (1): Myristica argentea, Myristica fragrans, Coriandrum sativum and Foeniculum vulgare

    Energy Technology Data Exchange (ETDEWEB)

    Hilmy, N.; Chosdu, R.; Sudiro, S. (National Atomic Energy Agency, Jakartc (Indonesia). Pasar Djumat Research Centre); Syuib, F. (Bandung Inst. of Tech. (Indonesia). Pharmacy Dept.)

    1981-10-01

    Medicinal plants i.e. dried seeds of myristica argentea, myristica fragrans, coriandrum sativum and foeniculum vulgare were stored for 0 and 6 months, with and without gamma irradiation at 5 kGy. No detectable changes were seen in infrared and UV spectra, GLC chromatograms and refraction indices of steam distilled, essential oils from the irradiated medicinal plants. The same results were also obtained from irradiated samples stored for 6 months at ambient conditions. Storage for 6 months caused significant changes in the gas liquid chromatograms and UV spectra of all samples compared to non-stored samples. The moisture content of all samples packed in polyethylene bags seems to be constant after 6 months of storage. No measurable changes were found in the amount of essential oil content caused by irradiation.

  12. Antioxidant, antihyperglycemic, and antihyperlipidemic effects of Coriandrum sativum leaf and stem in alloxan-induced diabetic rats.

    Science.gov (United States)

    Sreelatha, S; Inbavalli, R

    2012-07-01

    In India's indigenous system of medicine, Coriandrum sativum (CS), commonly used as a food ingredient, is claimed to be useful for various ailments. To establish its utility in diabetes mellitus, the present study evaluated the antidiabetic and antioxidant effects of CS in alloxan-induced diabetic rats. The extracts were shown to contain bioactive compounds including phenolics, flavonoids, steroids, and tannins. The extracts of CS in alloxan-induced diabetic rats were found to significantly lower blood glucose levels. Antidiabetic activity of the CS extracts was compared with the clinically available drug glibenclamide. The levels of serum total cholesterol, triglycerides, and low-density lipoprotein cholesterol were lower in the extract-treated group and high-density lipoprotein cholesterol was higher than the diabetic control rats. The extracts of CS exhibited strong scavenging effect on 2, 2-diphenyl-2-picrylhydrazyl free radical and inhibition of lipid peroxidation. The free radical scavenging effect of the extracts was comparable with that of the reference antioxidants. Furthermore, it also showed an improved antioxidant potential as evidenced by decreased lipid peroxidation and a significant increase in the activity of various antioxidant enzymes such as catalase, superoxide dismutase, and glutathione peroxidase in the liver of diabetic rats. These results indicate that the extracts could protect liver function and exhibited hypoglycemic, hypolipidemic, and antioxidant efficacies in the diabetic rats. These results support the use of this plant extract to manage diabetes mellitus. The leaves and stem of this plant Coriandrum sativum if used in cuisine would be a remedy for diabetes. © 2012 Institute of Food Technologists®

  13. Effects of hydroalcoholic extract of Coriandrum sativum on oxidative damage in pentylenetetrazole-induced seizures in rats.

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    Reza Karami

    2015-06-01

    Full Text Available An important role for oxidative stress, as a consequence of epileptic seizures, has been suggested. Coriandrum sativum has been shown that have antioxidant effects. Central nervous system depressant effects of C. sativum have also been reported. In this study, the effects of hydroalcoholic extract of aerial parts of the plants on brain tissues oxidative damages following seizures induced by pentylenetetrazole (PTZ was investigated in rats.The rats were divided into five groups and treated: (1 Control (saline, (2 PTZ (90 mg/kg, i.p., (3-5 three doses (100, 500 and 1000 mg/kg of C. sativum extract (CSE before PTZ. Latencies to the first minimal clonic seizures (MCS and the first generalized tonic-clonic seizures (GTCS were recorded. The cortical and hippocampal tissues were then removed for biochemical measurements.The extract significantly increased the MCS and GTCS latencies (P < 0.01, P < 0.001 following PTZ-induced seizures. The malondialdehyde (MDA levels in both cortical and hippocampal tissues of PTZ group were significantly higher than those of the control animals (P < 0.001. Pretreatment with the extract prevented elevation of the MDA levels (P < 0.010-P < 0.001. Following PTZ administration, a significant reduction in total thiol groups was observed in both cortical and hippocampal tissues (P < 0.050. Pre-treatment with the 500 mg/kg of the extract caused a significant prevention of decreased in total thiol concentration in the cortical tissues (P < 0.010.The present study showed that the hydroalcoholic extract of the aerial parts of C. sativum possess significant antioxidant and anticonvulsant activities.

  14. Separation and Identification of Erwinia caratovora ssp . caratovora Pathogen of Coriandrum sativum L%芫荽软腐病病原菌分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    张立微; 张景涛; 李小梅

    2015-01-01

    为有效防治芫荽软腐病,提高其产质量,通过对引起芫荽腐烂而绝产的病害的病原菌进行分离、纯化,对19个菌株进行17项生理生化指标测定。结果表明:根据伯杰氏细菌鉴定手册(第八版)以 Ecc为对照进行鉴定,确定E1(Ames24923)、E3(Ames24927)、E4(CORI107)、E7(CORI139)、E8(CORI140)、E10(CORI147)、E12(CORI289)、E13(CORI318)、E18(PI664510)为芫荽胡萝卜欧文氏杆菌属胡萝卜软腐欧文氏杆菌胡萝卜软腐病亚种(Erw inia carotovora ssp .carotovora ,Ecc)细菌,而其余供试菌株通过测定可确定为细菌,但属何种还需进一步鉴定。%In order to control the Erwinia caratovora ssp .caratovora of Coriandrum sativum L .,and improve the quality ,through the isolating and purificating of the pathogen which causes coriander decay ,19 strains were isolated and purified .The results showed that according to Bergey's Manual of Systematic Bacteriology (eighth edition) and 17 tests of physiology and biochemistry ,9 strainss were Erwiniacarotovora ssp .carotovora ,inclu‐ding E1(Ames24923) ,E3(Ames24927) ,E4(CORI107) ,E7(CORI139) ,E8(CORI140) ,E10(CORI147) ,E12 (CORI289) ,E13(CORI318) ,E18(PI664510) .Other strains should to be identified by other tools .

  15. Sleep-prolonging effect of Coriandrum sativum hydro-alcoholic extract in mice.

    Science.gov (United States)

    Rakhshandeh, Hassan; Sadeghnia, Hamid Reza; Ghorbani, Ahmad

    2012-01-01

    The present study was planned to investigate sleep-prolonging effect of C. sativum. The hydro-alcoholic extract (HAE) and its three fractions namely water (WF), ethyl acetate (EAF) and N-butanol (NBF) were prepared from C. sativum aerial parts and administrated to mice. Also, the possible cytotoxicity of the extracts was tested using cultured PC12 cells. The HAE, EAF and NBF significantly prolonged sleep duration. Only the NBF could significantly decrease sleep latency. No decrease in the neuronal surviving was observed either by HAE or by its fractions. The present data indicate that C. sativum exert sleep-prolonging action without major neurotoxic effect.

  16. Cognitive-enhancing and antioxidant activities of inhaled coriander volatile oil in amyloid β(1-42) rat model of Alzheimer's disease.

    Science.gov (United States)

    Cioanca, Oana; Hritcu, Lucian; Mihasan, Marius; Hancianu, Monica

    2013-08-15

    Coriandrum sativum L., commonly known as coriander and belonging to the Apiaceae family is cultivated throughout the world for its nutritional value. In traditional medicine, coriander is recommended for the relief of pain, anxiety, flatulence, loss of appetite and convulsions. In the present study, the effects of inhaled coriander volatile oil (1% and 3%, daily, for 21days) extracted from C. sativum var. microcarpum on spatial memory performance were assessed in an Aβ(1-42) rat model of Alzheimer's disease. The Aβ(1-42)-treated rats exhibited the following: decrease of spontaneous alternations percentage within Y-maze task and increase of working memory errors, reference memory errors and time taken to consume all five baits within radial arm maze task. Exposure to coriander volatile oil significantly improved these parameters, suggesting positive effects on spatial memory formation. Assessments of oxidative stress markers in the hippocampal tissue of Aβ(1-42)-treated rats showed a significant increase of superoxide dismutase (SOD), lactate dehydrogenase (LDH) and a decrease of glutathione peroxidase (GPX) specific activities along with an elevation of malondialdehyde (MDA) level. Coriander volatile oil significantly decreased SOD and LDH specific activities, increased GPX specific activity and attenuated the increased MDA level. Also, DNA cleavage patterns were absent in the coriander rats, thus suggesting antiapoptotic activity of the volatile oil. Therefore, our results suggest that exposure to coriander volatile oil ameliorates Aβ(1-42)-induced spatial memory impairment by attenuation of the oxidative stress in the rat hippocampus.

  17. Evaluation of anxiolytic activity of aqueous extract of Coriandrum sativum Linn. in mice: A preliminary experimental study

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    K Latha

    2015-01-01

    Full Text Available Objectives: To evaluate the anxiolytic effect of Coriandrum sativum (CS aqueous extract in mice. To compare the antianxiety activity of CS against standard drug diazepam (3 mg/kg. Materials and Methods: After obtaining Institutional Animal Ethics Committee approval, Swiss albino mice (18-25 g of either sex were randomly divided into five groups of six animals each. Dried powder of CS leaves was boiled with distilled water, cooled, filtered, placed on a hotplate for complete evaporation, finally weighed and stored. The control group, test group, and standard drugs group received saline, CS extract (50, 100, and 200 mg/kg, diazepam (3 mg/kg, respectively, by oral feeding. The antianxiety effect was assessed by elevated plus maze (EPM in mice. Results: In EPM, it implied that CS 50 mg/kg (Group III, 100 mg/kg (Group IV, and 200 mg/kg (Group V significantly (P < 0.001 increases the number of entries in open arms compared to control. The time spent in open arms also increased in all the doses of CS extract significantly. Conclusion: The current study demonstrates statistically significant dose-dependent antianxiety activity of CS leaves.

  18. Irradiation and chlorination effectively reduces Escherichia coli O157:H7 inoculated on cilantro (Coriandrum sativum) without negatively affecting quality.

    Science.gov (United States)

    Foley, Denise; Euper, Megan; Caporaso, Fred; Prakash, Anuradha

    2004-10-01

    Cilantro (Coriandrum sativum) inoculated with Escherichia coli O157:H7 at levels approximating 10(7) CFU/g was dipped in 200 ppm chlorine solution followed by low-dose gamma irradiation. Samples were plated on tryptic soy agar containing 50 microg/ml nalidixic acid (TSAN) as well as TSAN plates with two 7-ml layers of basal yeast extract agar (TSAN-TAL). Levels of E. coli O157:H7 recovered from both types of media were determined over 11 days. Chlorination alone reduced counts by just over 1.0 log cycle, whereas irradiation at 1.05 kGy resulted in a 6.7-log reduction, and a combination of irradiation and chlorination reduced counts more than 7 log cycles. Trained panels performed analytical sensory tests at time intervals for 14 days to detect changes in yellowing, tip burn, browning, black rot, sliminess, off-aroma, and off-flavor. Sensory tests found no significant differences among attributes over time or dose in samples irradiated at 1.08 to 3.85 kGy. This study showed that combination treatments of chlorination and low-dose irradiation can significantly reduce levels of E. coli O157:H7 in fresh cilantro while maintaining product quality.

  19. Effects of Coriandrum sativum extract on exploratory behaviour pattern and locomotor activity in mice: An experimental study

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    S N Harsha

    2012-01-01

    Full Text Available Objective : To investigate the effect of the hydroalcoholic extract obtained from Coriandrum sativum leaves on the exploratory behaviour pattern and locomotor activity of mice. Materials and Methods: Elevated plus maze (EPM and open field test (OFT were the screening tests used to assess the anxiolytic activity of , the extracts on mice. Diazepam (1 mg / kg served as the standard anxiolytic agent. The animal receiving extracts or diazepam showed an increase in the time spent, total entries in the open arm of the EPM and increased total locomotion in the OFT, suggesting anxiolytic activity. Results: The crude dried extract was prepared in doses of 100, 200 and 400 mg / kg body weight and administered intraperitoneally to the mice, for evaluation of the anxiolytic activity. The 200 and 400 mg / kg body weight produced highly significant (P < 0.01 anxiolytic effects, in a dose-dependent manner, by increasing the time spent on and the number of entries into the open arms of the EPM and by an increase in the locomotion by mice in the OFT. Furthermore, in lower doses the extract did not affect the locomotor activity. Conclusion: Our findings demonstrated that the leaf extract of the plant exerted an anti-anxiety effect on mice in the elevated plus maze and open field test.

  20. Coriandrum sativum L. seed extract mitigates lipotoxicity in RAW 264.7 cells and prevents atherogenic changes in rats.

    Science.gov (United States)

    Patel, Dipak; Desai, Swati; Gajaria, Tejal; Devkar, Ranjitsinh; Ramachandran, A V

    2013-01-01

    This study was designed to assess the efficacy of Coriandrum sativum L. (CS) in preventing in vitro low density lipoprotein (LDL) oxidation mediated macrophage modification. Further, an in vivo study was also conducted to confirm upon the efficacy of CS seed extract in alleviating pathophysiological alterations of high cholesterol diet induced atherosclerosis in rats. Copper mediated cell free oxidation of LDL accounted for elevated indices of malondialdehyde (MDA), lipid hydroperoxide (LHP)and protein carbonyl (PC) and a progressive increment in conjugate diene (CD) levels whereas, reverse set of changes were recorded in presence of CS extract. Cell mediated LDL oxidation (using RAW 264.7 cells) accounted for lowered MDA production and oxidized LDL (Ox-LDL) mediated cell death in presence of CS extract and the same was attributed to its potent antioxidant and free radical scavenging potentials. High cholesterol fed atherogenic rats showed elevated lipid indices, evidences of LDL oxidation, plaque formation in thoracic aorta. The same was further validated with immunostaining of cell adhesion molecules and hematoxylin and eosin (HXE) staining. However, co-supplementation of CS to atherogenic rats recorded significant lowering of the above mentioned parameters further strengthening the claim that CS extract is instrumental in preventing onset and progression of atherosclerosis.

  1. Acute and sub-chronic toxicological evaluation of hydro-methanolic extract of Coriandrum sativum L. seeds.

    Science.gov (United States)

    Patel, Dipak; Desai, Swati; Devkar, Ranjitsinh; Ramachandran, A V

    2012-01-01

    Coriandrum sativum L. (CS) seeds are known to possess therapeutic potentials against a variety of physiological disorders. This study assesses acute and sub-chronic toxicity profile of hydro-methanolic extract of CS seeds using OECD guidelines. In acute toxicity study, mice were once orally administered 1000, 3000 and 5000 mg/kg body weight of CS extract. There were no any behavioral alterations or mortality recorded in CS treated groups. The LD50 value was more than 5000 mg/kg body weight. In the sub-chronic oral toxicity study, the animals were orally administered with CS extract (1000, 2000 and 3000mg/kg body weight) daily for 28 days whereas; vehicle control group received 0.5 % carboxy methyl cellulose. There was significant reduction in food intake, body weight gain and plasma lipid profiles of CS2 and CS3 (2000 and 3000 mg/kg body weight respectively) groups as compared to the control group. However, there were no alterations in haematological profile, relative organ weights, histology and plasma markers of damage of vital organs (heart, liver and kidney). The overall finding of this study indicates that CS extract is non-toxic up to 3000 mg/kg body weight and can be considered as safe for consumption.

  2. In vitro efficacy of Coriandrum sativum, Lippia sidoides and Copaifera reticulata against Leishmania chagasi Eficácia in vitro de Coriandrum sativum, Lippia sidoides e Copaifera reticulata sobre Leishmania chagasi

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    Fernanda Cristina Macedo Rondon

    2012-09-01

    Full Text Available The increased incidence of visceral leishmaniasis (VL in Brazil is due to a lack of effective disease control measures. In addition to that, no effective treatment exists for canine VL in response to synthetic drugs. Thus, the objective of this study was to evaluate the effect of the essential oils of Coriandrum sativum and Lippia sidoides, and oleoresin from Copaifera reticulata, on Leishmania chagasi promastigotes and amastigotes. We also examined the toxicity of these treatments on the murine monocyte cell line RAW 264.7. To determine the IC50 a MTT test (3-(4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide was performed on promastigotes, and an in situ ELISA assay was conducted on amastigotes. Here, we demonstrate that oleoresin from C. reticulata was effective against both promastigotes (IC50 of 7.88 µg.mL-1 and amastigotes (IC50 of 0.52 µg.mL-1, and neither of the two treatments differed significantly (p > 0.05 from pentamidine (IC50 of 2.149 µg.mL-1 and amphotericin B (IC50 of 9.754 µg.mL-1. Of the three plant oils tested, only oleoresin showed no toxicity toward monocyte, with 78.45% viability after treatment. Inhibition of promastigote and amastigote growth and the lack of cytotoxicity by C. reticulata demonstrate that oleoresin may be a viable option for analyzing the in vivo therapeutic effects of leishmanicidal plantsO aumento na incidência da Leishmaníase Visceral (LV no Brasil deve-se à ineficácia das medidas de controle da doença. Além disso, não há tratamento efetivo para LV canina com drogas sintéticas. Assim, o objetivo deste trabalho foi avaliar o efeito dos óleos essenciais de Coriandrum sativum e de Lippia sidoides e do óleo-resina de Copaiferareticulata sobre promastigotas e amastigotas de Leishmania chagasi e analisar o grau de toxicidade sobre células monocíticas murinas RAW 264.7. Para determinar a CI50 sobre promastigotas foi usado teste MTT (brometo de 3-[4,5-dimetil-tiazol-2-il]-2

  3. Effect of phytohormones on seed germination and seedling growth of Coriandrum sativum L.

    Science.gov (United States)

    Kumar, Mahender; Agnihotri, R K; Vamil, R; Sharma, R

    2014-04-01

    Coriander commonly known as Dhania or Chinese parsley is generally grown for its use in soups, salads, dressing vegetables, seasoning and chutney. Effect of two phytohormones viz. GA3 and 2,4-D on seed germination, seedling growth and various physiological and biochemical parameters were studied. The hormones were applied individually in different concentrations (10, 50 and 100 μM concentrations). Both the hormones enhanced the germination percentage, seedling growth (root and shoot length), leaf area, chlorophyll and carotenoid content. The application of these hormones also decreased the germination time. Maximum germination, shoot length, leaf area and carotenoid content was observed in 100 μm concentration of GA3. Root length, chl. a and chl. b was maximum in 50 μM of 2,4-D and 100 μm GA3, respectively. The application of two hormones exhibited a marked increase on all the parameters studied as compared to the control.

  4. Respuesta fisiológica de cilantro (Coriandrum sativum L. a la disponibilidad de agua en el suelo

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    María Sara Mejía de Tafur

    2014-09-01

    Full Text Available En casa de malla de la Universidad Nacional de Colombia sede Palmira (3° 30' 45.6" N y 76° 18' 29.911" O, 1050 m.s.n.m., 23.5 °C y 77% de HR se midieron los requerimientos hídricos del cilantro (Coriandrum sativum L. variedad Unapal Precoso y se relacionaron con el rendimiento de follaje fresco y materia seca. Las plantas fueron sembradas en macetas con 6 kg de suelo y por ciclo de cultivo se aplicaron láminas de agua de 140, 160, 200 (testigo propuesta por Vallejo y Estrada, 2004, 240 y 280 mm. Se utilizó un diseño completamente al azar con cinco tratamientos, cuatro repeticiones y 36 plantas por unidad experimental. Cada día, durante la fase experimental, se realizaron mediciones de evapotranspiración y cada 35 días después de la siembra se midieron las producciones de follaje y biomasa. La mayor producción de follaje se obtuvo con 200 mm de agua, valor asociado con la mayor eficiencia en el uso de ésta, medida como producción de MS, (0.64 g/lt de agua aplicado. En la etapa de germinación el coeficiente Kc del cultivo (relación evaporación real/evaporación de referencia fue de 0.83, 1.12 en la etapa de crecimiento lineal y 1.40 en la etapa de formación de tallo floral y cosecha.

  5. Diversity of root associated microorganisms of selected medicinal plants and influence of rhizomicroorganisms on the antimicrobial property of Coriandrum sativum.

    Science.gov (United States)

    Tamilarasi, S; Nanthakumar, K; Karthikeyan, K; Lakshmanaperumalsamy, P

    2008-01-01

    The total heterotrophic bacteria, actinomycetes and fungus were enumerated from the rhizosphere and non-rhizosphere soil of 50 selected locally available medicinal plants in and around Bharathiar University. In all the plants, population of microorganism were higher in the rhizosphere soil than in the non rhizosphere soil. Among the microorganisms, bacterial population was higher in number followed by fungus and actinomycetes. Of the medicinal plants, the maximum rhizosphere effect was observed in Annona squamosa and the minimum effect was seen in Eclipta alba and Cassia auriculata. Among the bacteria the dominant species was Bacillus followed by Pseudomonas, Enterobacter, Corynebacterium, Micrococcus and Serratia. The Streptomyces species was found to be dominant followed by Deuteromycetes and Frankia among the actinomycetes. Among the fungal isolates Rhizopus was found to be higher in number followed by Aspergillus, Penicillium, Mucor and Fusarium. About 70.96% of the bacterial isolates were found to be nitrate reducers and 90.60% of the bacteria solubilised phosphate. The rhizosphere bacterial isolates were also capable of hydrolyzing starch, cellulose, casein, urea and gelatin. The isolates of bacteria, actinomycetes and fungus were also able to produce phytohormone Indole-3-acetic acid (IAA). The maximum IAA production was recorded by Fusarium sp (5.8 mg/l). The rhizosphere bacterial isolates showed resistance to 14 commercially used antibiotics. In an attempt to check the influence of these plant growth promoting microorganisms on the antimicrobial property of Coriandrum sativum against Escherichia coli MTCC-443 and Aeromonas hydrophila MTCC-646, the results observed was not encouraging since the inoculants did not influence the antibacterial property. However extensive and in depth study is required to find out the influence of rhizomicroorganisms on the antibacterial property of medicinal plants. The other results clearly indicated that the rhizosphere

  6. Antifungal activity, toxicity and chemical composition of the essential oil of Coriandrum sativum L. fruits.

    Science.gov (United States)

    Soares, Bruna V; Morais, Selene M; dos Santos Fontenelle, Raquel Oliveira; Queiroz, Vanessa A; Vila-Nova, Nadja S; Pereira, Christiana M C; Brito, Edy S; Neto, Manoel A S; Brito, Erika H S; Cavalcante, Carolina S P; Castelo-Branco, Débora S C M; Rocha, Marcos F G

    2012-07-11

    The aims of this study were to test the antifungal activity, toxicity and chemical composition of essential oil from C. sativum L. fruits. The essential oil, obtained by hydro-distillation, was analyzed by gas chromatography/mass spectroscopy. Linalool was the main constituent (58.22%). The oil was considered bioactive, showing an LC₅₀ value of 23 μg/mL in the Artemia salina lethality test. The antifungal activity was evaluated against Microsporum canis and Candida spp. by the agar-well diffusion method and the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were established by the broth microdilution method. The essential oil induced growth inhibition zones of 28 ± 5.42 and 9.25 ± 0.5 for M. canis and Candida spp. respectively. The MICs and MFCs for M. canis strains ranged from 78 to 620 and 150 to 1,250 μg/mL, and the MICs and MFCs for Candida spp strains ranged from 310 to 620 and 620 to 1,250 μg/mL, respectively. C. sativum essential oil is active in vitro against M. canis and Candida spp. demonstrating good antifungal activity.

  7. Comparative study of lipophilic and hydrophilic antioxidants from in vivo and in vitro grown Coriandrum sativum.

    Science.gov (United States)

    Dias, Maria Inês; Barros, Lillian; Sousa, Maria João; Ferreira, Isabel C F R

    2011-06-01

    Coriander is commonly used for medicinal purposes, food applications, cosmetics and perfumes. Herein, the production of antioxidants in vegetative parts (leaves and stems) of in vivo and in vitro grown samples was compared. In vitro samples were clone A- with notorious purple pigmentation in stems and leaves and clone B- green. Seeds were also studied as they are used to obtain in vivo and in vitro vegetative parts. Lipophilic (tocopherols, carotenoids and chlorophylls) and hydrophilic (sugars, ascorbic acid, phenolics, flavonols and anthocyanins) compounds were quantified. The antioxidant activity was evaluated by radical scavenging activity, reducing power and lipid peroxidation inhibition. The in vivo sample showed the highest antioxidant activity mainly due to its highest levels of hydrophilic compounds. Otherwise, in vitro samples, mainly clone A, gave the highest concentration in lipophilic compounds but a different profile when compared to the in vivo sample. Clones A and B revealed a lack of β-carotene, β- and δ-tocopherols, a decrease in α-tocopherol, and an increase in γ-tocopherol and clorophylls in comparison to the in vivo sample. In vitro culture might be useful to explore the plants potentialities for industrial applications, controlling environmental conditions to produce higher amounts of some bioactive products.

  8. Evaluation of antibacterial activity of Coriandrum sativum (L. against gram and ndash; positive and gram and ndash; negative bacteria

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    D. Sambasivaraju

    2016-12-01

    Conclusions: Coriander oil demonstrated good anti-bacterial activity against commonly five occurring microorganisms but further extensive studies are required to isolate the active ingredient responsible for antibacterial action and develop coriander essential oil as a clinically proven antibacterial agent. [Int J Basic Clin Pharmacol 2016; 5(6.000: 2653-2656

  9. Antimicrobial activity of coriander oil and its effectiveness as food preservative.

    Science.gov (United States)

    Silva, Filomena; Domingues, Fernanda C

    2017-01-02

    ABTRACT Foodborne illness represents a major economic burden worldwide and a serious public health threat, with around 48 million people affected and 3,000 death each year only in the USA. One of the possible strategies to reduce foodborne infections is the development of effective preservation strategies capable of eradicating microbial contamination of foods. Over the last years, new challenges for the food industry have arisen such as the increase of antimicrobial resistance of foodborne pathogens to common preservatives and consumers demand for naturally based products. In order to overcome this, new approaches using natural or bio-based products as food preservatives need to be investigated. Coriander (Coriandrum sativum L.) is a well-known herb widely used as spice, or in folk medicine, and in the pharmacy and food industries. Coriander seed oil is the world's second most relevant essential oil, exhibiting antimicrobial activity against Gram-positive and Gram-negative bacteria, some yeasts, dermatophytes and filamentous fungi. This review highlights coriander oil antimicrobial activity and possible mechanisms of action in microbial cells and discusses the ability of coriander oil usage as a food preservative, pointing out possible paths for the successful evolution for these strategies towards a successful development of a food preservation strategy using coriander oil.

  10. Hydrodistillation extraction time effect on essential oil yield, composition, and bioactivity of coriander oil.

    Science.gov (United States)

    Zheljazkov, Valtcho D; Astatkie, Tess; Schlegel, Vicki

    2014-01-01

    Coriander (Coriandrum sativum L.) is a major essential oil crop grown throughout the world. Coriander essential oil is extracted from coriander fruits via hydrodistillation, with the industry using 180-240 min of distillation time (DT), but the optimum DT for maximizing essential oil yield, composition of constituents, and antioxidant activities are not known. This research was conducted to determine the effect of DT on coriander oil yield, composition, and bioactivity. The results show that essential oil yield at the shorter DT was low and generally increased with increasing DT with the maximum yields achieved at DT between 40 and 160 min. The concentrations of the low-boiling point essential oil constituents: α-pinene, camphene, β-pinene, myrcene, para-cymene, limonene, and γ-terpinene were higher at shorter DT (essential oil constituent, linalool, was 51% at DT 1.15 min, and increased steadily to 68% with increasing DT. In conclusion, 40 min DT is sufficient to maximize yield of essential oil; and different DT can be used to obtain essential oil with differential composition. Its antioxidant capacity was affected by the DT, with 20 and 240 min DT showing higher antioxidant activity. Comparisons of coriander essential oil composition must consider the length of the DT.

  11. Influence of the isolation procedure on coriander leaf volatiles with some correlation to the enzymatic activity.

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    To Quynh, Cung Thi; Iijima, Yoko; Kubota, Kikue

    2010-01-27

    Coriander leaves (Coriandrum sativum L.) have become popular worldwide because of their pleasant and delicate aroma. By a hot water extraction method, in which coriander leaves were cut before suspending in boiling water for 2 min, the contents of the main volatile compounds such as alkanals and 2-alkenals from C10 to C14 decreased, while the levels of corresponding alcohols increased in comparison to those obtained by solvent extraction. To investigate the reasons for this variation, an enzyme activity was assayed. By using aliphatic aldehyde as a substrate and NADPH as a coenzyme, strong activity of an aliphatic aldehyde reductase was found for the first time in this herb in the relatively wide pH range of 5.0-9.0, with the maximum activity at pH 8.5. Additionally, the aliphatic aldehyde dehydrogenase, responsible for acid formation, was also found to have a relatively weak activity compared to that of reductase.

  12. Study of the major essential oil compounds of Coriandrum sativum against Acinetobacter baumannii and the effect of linalool on adhesion, biofilms and quorum sensing.

    Science.gov (United States)

    Alves, Susana; Duarte, Andreia; Sousa, Sónia; Domingues, Fernanda C

    2016-01-01

    Acinetobacter baumannii is a pathogen that has the ability to adhere to surfaces in the hospital environment and to form biofilms which are increasingly resistant to antimicrobial agents. The aim of this work was to study the antimicrobial activity of the major oil compounds of Coriandrum sativum against A. baumannii. The effect of linalool on planktonic cells and biofilms of A. baumannii on different surfaces, as well as its effect on adhesion and quorum sensing was evaluated. From all the compounds evaluated, linalool was the compound with the best antibacterial activity, with minimum inhibitory concentration values between 2 and 8 μl ml(-1). Linalool also inhibited biofilm formation and dispersed established biofilms of A. baumannii, changed the adhesion of A. baumannii to surfaces and interfered with the quorum- sensing system. Thus, linalool could be a promising antimicrobial agent for controlling planktonic cells and biofilms of A. baumannii.

  13. 反季节芫荽品比试验初报%Variety Comparative Test for Off-season Coriandrum sativum

    Institute of Scientific and Technical Information of China (English)

    赵基英; 杨条江; 陈兴华

    2004-01-01

    芫荽(Coriandrum sativum)又叫香菜.性喜冷凉.为全国性蔬菜。一年四季都可种植,露地栽培春秋两季较多.夏季供不应求,市场效益好。为筛选出适合贵阳地区反季节栽培、不易抽薹、不老化的优质、高产、抗逆性强的芫荽品种.根据芫荽的生长特性,我们于2003年4~6月在贵阳市小河区进行反季节栽培。

  14. Stability Analysis for Seed Yield over Environments in Coriander

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    Sangeeta Yadav

    2016-11-01

    Full Text Available Thirty five genotypes of coriander (Coriandrum sativum L. were tested in four artificially created environments to judge their stability in performance of seed yield. The differences among genotypes and environments were significant for seed yield. Stability parameters varied considerably among the tested genotypes in all the methods used. The variation in result in different methods was due to non-fulfillment of assumption of different models. However, AMMI analysis provides the information on main effects as well as interaction effects and depiction of PCA score gives better understanding of the pattern of genotype – environment interaction. The sum of squares due to PCAs was also used for the computation of AMMI stability values for better understanding of the adaptability behavior of genotypes hence, additive main effects and multiplicative interaction (AMMI model was most appropriate for the analysis of G x E interactions for seed yield in coriander. Genotypes RVC 15, RVC 19, RVC 22, RVC 25 and Panipat local showed wider adaptability while, Simpo S 33 exhibited specific adaptability to favourable conditions of high fertility. These genotypes could be utilized in breeding programmers to transfer the adaptability genes into high yielding genetic back ground of coriander.

  15. The Effects of Drought Stress on Morphological Traits and Yield of Three Medicinal Plants ( Coriandrum sativum, Foeniculum vulgare and Anethum graveolens in Greenhouse Conditions.

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    S. R Amiri Deh Ahmadi

    2012-07-01

    Full Text Available Drought stress is one of the important problems in arid and semi_arid regions. drought stress take place in plants when available water is lesser than evapotranspiration. In order to evaluate the effect of drought stress on morphological traits and yield of three medicinal plants an experiment was carried out during the 2010 year in research greenhouse of faculity of Agriculture, Ferdowsi University of Mashhad, in a factoriel arrengement based on a Completely Randomized Block Design with four replications. factors include 4 drought stress levels: Field capacity (100%, 75%, 50% and FC25% and 3 medicinal plants include: Coriandrum sativum, Foeniculum vulgare and Aniethum graveolens. The traits such as plant height, leaf number per plant, number of lateral branches per plant, umbrellas per plant, umbels per umbrellas, seed number per plant, seed weight per plant, 1000 seeds weight and harvest index were measured. Results showed that decreasing content of water in soil lesser than field capacity had significant effects on all of these traits. Coriandrum sativum in FC25% had maximum seed number in umbrellas(14 number, seed number per plant(27 number, Umbrellas per plant(4 number, umbels per umbrellas(10 number and 1000 seeds weight (6.5 gr. also Foeniculum vulgare in FC25% had minimum seed number in umbrellas(2 number, seed number per plant(2 number. Umbrellas per plant(0.25number, umbels per umbrellas(0.5 number and 1000 seeds weight (0.15 gr. Harvest index positive correlation with seed weight per plant and 1000 seeds weight.

  16. Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils.

    Science.gov (United States)

    Delaquis, Pascal J; Stanich, Kareen; Girard, Benoit; Mazza, G

    2002-03-25

    Essential oils from dill (Anethum graveolens L.), coriander (seeds of Coriandrum sativum L.), cilantro (leaves of immature C. sativum L.) and eucalyptus (Eucalyptus dives) were separated into heterogeneous mixtures of components by fractional distillation and were analyzed by gas chromatography-mass spectroscopy. Minimum inhibitory concentrations against gram-positive bacteria, gram-negative bacteria and Saccharomyces cerevisiae were determined for the crude oils and their fractions. Essential oil of cilantro was particularly effective against Listeria monocytogenes, likely due to the presence of long chain (C6-C10) alcohols and aldehydes. The strength and spectrum of inhibition for the fractions often exceeded those determined in the crude oils. Mixing of fractions resulted in additive, synergistic or antagonistic effects against individual test microorganisms.

  17. Arbuscular mycorrhizal fungi are an alternative to the application of chemical fertilizer in the production of the medicinal and aromatic plant Coriandrum sativum L.

    Science.gov (United States)

    Oliveira, Rui S; Ma, Ying; Rocha, Inês; Carvalho, Maria F; Vosátka, Miroslav; Freitas, Helena

    2016-01-01

    The widespread use of agrochemicals is detrimental to the environment and may exert harmful effects on human health. The consumer demand for organic food plants has been increasing. There is thus a rising need for alternatives to agrochemicals that can foster sustainable plant production. The aim of this study was to evaluate the potential use of an arbuscular mycorrhizal (AM) fungus as an alternative to application of chemical fertilizer for improving growth performance of the medicinal and aromatic plant Coriandrum sativum. Plants were inoculated with the AM fungus Rhizophagus irregularis BEG163 and/or supplemented with a commercial chemical fertilizer (Plant Marvel, Nutriculture Bent Special) in agricultural soil. Plant growth, nutrition, and development of AM fungus were assessed. Plants inoculated with R. irregularis and those supplemented with chemical fertilizer displayed significantly improved growth performances when compared with controls. There were no significant differences in total fresh weight between plants inoculated with R. irregularis or those supplemented with chemical fertilizer. Leaf chlorophyll a + b (82%), shoot nitrogen (44%), phosphorus (254%), and potassium (27%) concentrations increased in plants inoculated with R. irregularis compared to controls. Application of chemical fertilizer inhibited root mycorrhizal colonization and the length of the extraradical mycelium of R. irregularis. Inoculation with R. irregularis was equally or more efficient than application of chemical fertilizer in promoting growth and nutrition of C. sativum. AM fungi may thus contribute to improve biologically based production of food plants and reduce the dependence on agrochemicals in agriculture.

  18. Drying and color characteristics of coriander foliage using convective thin-layer and microwave drying.

    Science.gov (United States)

    Shaw, Mark; Meda, Venkatesh; Tabil, Lope; Opoku, Anthony

    2007-01-01

    Heat sensitive properties (aromatic, medicinal, color) provide herbs and spices with their high market value. In order to prevent extreme loss of heat sensitive properties when drying herbs, they are normally dried at low temperatures for longer periods of time to preserve these sensory properties. High energy consumption often results from drying herbs over a long period. Coriander (Coriandrum sativum L., Umbelliferae) was dehydrated in two different drying units (thin layer convection and microwave dryers) in order to compare the drying and final product quality (color) characteristics. Microwave drying of the coriander foliage was faster than convective drying. The entire drying process took place in the falling rate period for both microwave and convective dried samples. The drying rate for the microwave dried samples ranged from 42.3 to 48.2% db/min and that of the convective dried samples ranged from 7.1 to 12.5% db/min. The fresh sample color had the lowest L value at 26.83 with higher L values for all dried samples. The results show that convective thin layer dried coriander samples exhibited a significantly greater color change than microwave dried coriander samples. The color change index values for the microwave dried samples ranged from 2.67 to 3.27 and that of the convective dried samples varied from 4.59 to 6.58.

  19. Antifungal activity of Coriandrum sativum essential oil, its mode of action against Candida species and potential synergism with amphotericin B.

    Science.gov (United States)

    Silva, Filomena; Ferreira, Susana; Duarte, Andreia; Mendonça, Dina I; Domingues, Fernanda C

    2011-12-15

    The increasing incidence of drug-resistant pathogens and toxicity of existing antifungal compounds has drawn attention towards the antimicrobial activity of natural products. The aim of the present study was to evaluate the antifungal activity of coriander essential oil according to classical bacteriological techniques, as well as with flow cytometry. The effect of the essential oil upon germ tube formation, seen as an important virulence factor, and potential synergism with amphotericin B were also studied. Coriander essential oil has a fungicidal activity against the Candida strains tested with MLC values equal to the MIC value and ranging from 0.05 to 0.4% (v/v). Flow cytometric evaluation of BOX, PI and DRAQ5 staining indicates that the fungicidal effect is a result of cytoplasmic membrane damage and subsequent leakage of intracellular components such as DNA. Also, concentrations bellow the MIC value caused a marked reduction in the percentage of germ tube formation for C. albicans strains. A synergetic effect between coriander oil and amphotericin B was also obtained for C. albicans strains, while for C. tropicalis strain only an additive effect was observed. This study describes the antifungal activity of coriander essential oil on Candida spp., which could be useful in designing new formulations for candidosis treatment.

  20. Antimicrobial activity against bacteria with dermatological relevance and skin tolerance of the essential oil from Coriandrum sativum L. fruits.

    Science.gov (United States)

    Casetti, F; Bartelke, S; Biehler, K; Augustin, M; Schempp, C M; Frank, U

    2012-03-01

    The aim of this work was to determine the antibacterial activity of essential coriander oil (ECO) on bacteria with dermatological relevance and to assess the skin tolerance of antimicrobial effective ECO concentrations. Essential coriander oil was tested on clinical isolates of different bacteria species, all of which may cause superficial skin infections. Antimicrobial susceptibility testing was performed using a standardized macrodilution test. Essential coriander oil showed good antibacterial activity towards the majority of the bacterial strains tested, including Streptococcus pyogenes (Lancefield group A) and methicillin resistant Staphylococcus aureus (MRSA), with mean minimal inhibitory concentrations of 0.04% v/v and 0.25% v/v, respectively. The skin tolerance of a cream and a lotion containing 0.5% and 1.0% ECO was assessed in 40 healthy volunteers using the occlusive patch test. No skin irritation could be observed by sensitive photometric assessment in any of the volunteers. Because of its activity against Streptococcus pyogenes, Staphylococcus aureus and MRSA combined with excellent skin tolerance, ECO might be useful as an antiseptic for the prevention and treatment of skin infections with Gram-positive bacteria. Copyright © 2011 John Wiley & Sons, Ltd.

  1. Inhalation of coriander volatile oil increased anxiolytic-antidepressant-like behaviors and decreased oxidative status in beta-amyloid (1-42) rat model of Alzheimer's disease.

    Science.gov (United States)

    Cioanca, Oana; Hritcu, Lucian; Mihasan, Marius; Trifan, Adriana; Hancianu, Monica

    2014-05-28

    The present study analyzed the possible anxiolytic, antidepressant and antioxidant proprieties of inhaled coriander volatile oil extracted from Coriandrum sativum var. microcarpum in beta-amyloid (1-42) rat model of Alzheimer's disease. The anxiolytic- and antidepressant-like effects of inhaled coriander volatile oil were studied by means of in vivo (elevated plus-maze and forced swimming tests) approaches. Also, the antioxidant activity in the hippocampus was assessed using catalase specific activity and the total content of the reduced glutathione. The beta-amyloid (1-42)-treated rats exhibited the following: decrease of the locomotor activity, the percentage of the time spent and the number of entries in the open arm within elevated plus-maze test and decrease of swimming and immobility times within forced swimming test. Exposure to coriander volatile oil significantly improved these parameters, suggesting anxiolytic- and antidepressant-like effects. Moreover, coriander volatile oil decreased catalase activity and increased glutathione level in the hippocampus. Our results suggest that multiple exposures to coriander volatile oil can be useful as a mean to counteract anxiety, depression and oxidative stress in Alzheimer's disease conditions.

  2. Anti-acne, anti-dandruff and anti-breast cancer efficacy of green synthesised silver nanoparticles using Coriandrum sativum leaf extract.

    Science.gov (United States)

    Sathishkumar, Palanivel; Preethi, Johnson; Vijayan, Raji; Mohd Yusoff, Abdull Rahim; Ameen, Fuad; Suresh, Sadhasivam; Balagurunathan, Ramasamy; Palvannan, Thayumanavan

    2016-10-01

    In this present investigation, AgNPs were green synthesised using Coriandrum sativum leaf extract. The physicochemical properties of AgNPs were characterised using UV-visible spectrophotometer, field emission scanning microscopy/energy dispersive X-ray (FESEM/EDX), Fourier transformed infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and Brunauer-Emmett-Teller (BET) analysis. Further, in vitro anti-acne, anti-dandruff and anti-breast cancer efficacy of green synthesised AgNPs were assessed against Propionibacterium acnes MTCC 1951, Malassezia furfur MTCC 1374 and human breast adenocarcinoma (MCF-7) cell line, respectively. The flavonoids present in the plant extract were responsible for the AgNPs synthesis. The green synthesised nanoparticles size was found to be ≈37nm. The BET analysis result shows that the surface area of the synthesised AgNPs was found to be 33.72m(2)g(-1). The minimal inhibitory concentration (MIC) of AgNPs for acne causative agent P. acnes and dandruff causative agent M. furfur was found to be at 3.1 and 25μgmL(-1), respectively. The half maximal inhibitory concentration (IC50) value of the AgNPs for MCF-7 cells was calculated as 30.5μgmL(-1) and complete inhibition was observed at a concentration of 100μgmL(-1). Finally, our results proved that green synthesised AgNPs using C. sativum have great potential in biomedical applications such as anti-acne, anti-dandruff and anti-breast cancer treatment.

  3. The influence of fertilization on yield of caraway, anise and coriander in organic agriculture

    Directory of Open Access Journals (Sweden)

    Aćimović Milica G.

    2013-01-01

    Full Text Available Many plants of Apiaceae family have long been well known because of flavorful aromatic spice and, because of its healing properties, are often used in folk medicine and in cooking. In our study three plants of this family were included: caraway (Carum carvi L., anise (Pimpinella anisum L. and coriander (Coriandrum sativum L.. Regarding good agro-ecological conditions for growing these plants in Serbia, and a new world trend of increasing organic agricultural production, the aim of this study was to investigate the effects of application of various types of fertilizers on yield of studied plant species. The field experiment was carried out during the years of 2011 and 2012, in three localities in Vojvodina Province with the local ecotypes and six different treatments (control, ‘Slavol’, ‘Bactofil B-10’, ‘Royal Ofert’, vermicompost and NPK. The highest caraway yield was obtained by the application of biofertilizer ‘Bactofil B-10’. As regards anise and coriander the highest yield was achieved by the application of chemical fertilizer. The most effective organic fertilizers were the following: vermicompost for anise and specific poultry manure ‘Royal Ofert’ granules for coriander.

  4. Análise Germinativa de Sementes Comerciais de Coentro (Coriandrum sativum L. no município de Serra Talhada - PE

    Directory of Open Access Journals (Sweden)

    Hamurábi Anizio Lins

    2015-06-01

    Full Text Available A análise germinativa em sementes de coentro (Coriandrum sativum L. tem o intuito de avaliar a qualidade fisiológica de sementes para fins de semeadura e comercialização de lotes. Diante disso, a pesquisa teve como objetivo analisar a germinação de sementes comerciais de coentro. O experimento foi conduzido no município de Serra Talhada, PE. Foram utilizadas duas variedades do coentro (Verdão e Portuguesa, sendo empregados dois tratamentos e dez repetições, contendo 30 sementes cada um. A semeadura foi feita em bandejas de isopor contento 200 células, preenchidas com o substrato comercial. Cada semente de coentro foi colocada no centro de cada célula da bandeja, na profundidade de um centímetro, sendo irrigadas diariamente. Adotou-se o Delineamento Inteiramente Casualizado (DIC. Para realizar as avaliações, foi coletada diariamente a quantidade de sementes emergidas. Os parâmetros avaliados foram porcentagem de emergência (PE, tempo médio de emergência (TME e velocidade média de emergência (VME. Os resultados foram submetidos à análise de variância, com a significância testada através do teste de Tukey, com até 5% de probabilidade. Verificou-se que a variedade Verdão diferenciou significativamente da variedade Portuguesa, concluindo assim que a porcentagem de emergência das variedades de coentro não condiz com o indicado pelo fabricante.

  5. Calidad microbiológica y parasitológica de lechuga (Lactuca sativa y cilantro (Coriandrum sativum expendidos en la Parroquia Santa Rita, Aragua, Venezuela

    Directory of Open Access Journals (Sweden)

    Nirza Noguera Machado

    2016-10-01

    Full Text Available Las hortalizas aportan grandes beneficios para la salud debido a sus innumerables propiedades alimenticias; sin embargo, existe una gran variedad de factores que ponen en riesgo la calidad sanitaria de las mismas constituyéndose en vehículos para enfermedades de transmisión alimentaria (ETA. Por ello, se decidió evaluar la calidad microbiológica y parasitológica de dos hortalizas, lechuga (Lactuca sativa y cilantro (Coriandrum sativum expendidas en la Parroquia Santa Rita del Municipio Francisco Linares Alcántara (Aragua, Venezuela. Se analizaron un total de 20 muestras, 10 de cilantro y 10 de lechuga; la determinación microbiológica se realizó por la técnica del Número Más Probable y el análisis parasitológico, mediante observación directa, tinción de Kinyoun y la técnica de Faust. Los resultados mostraron la presencia de coliformes totales (1,1x104 NMP/g en el 100 % de las muestras. No se evidenció coliformes fecales en ninguna de las muestras. En relación al análisis parasitológico, se identificaron protozoarios intestinales, con mayor prevalencia de Blastocystis spp. y menor en helmintos. Los resultados demuestran el peligro potencial que representa el consumo de hortalizas frescas contaminadas para la salud pública. Se debe concientizar a la población sobre medidas sanitarias preventivas que deben aplicar antes del consumo e implementar medidas de control que mejoren las condiciones higiénicas en la cadena de comercialización.

  6. Antifungal Activity, Toxicity and Chemical Composition of the Essential Oil of Coriandrum sativum L. Fruits

    Directory of Open Access Journals (Sweden)

    Erika H. S. Brito

    2012-07-01

    Full Text Available The aims of this study were to test the antifungal activity, toxicity and chemical composition of essential oil from C. sativum L. fruits. The essential oil, obtained by hydro-distillation, was analyzed by gas chromatography/mass spectroscopy. Linalool was the main constituent (58.22%. The oil was considered bioactive, showing an LC50 value of 23 µg/mL in the Artemia salina lethality test. The antifungal activity was evaluated against Microsporum canis and Candida spp. by the agar-well diffusion method and the minimum inhibitory concentration (MIC and the minimum fungicidal concentration (MFC were established by the broth microdilution method. The essential oil induced growth inhibition zones of 28 ± 5.42 and 9.25 ± 0.5 for M. canis and Candida spp. respectively. The MICs and MFCs for M. canis strains ranged from 78 to 620 and 150 to 1,250 µg/mL, and the MICs and MFCs for Candida spp strains ranged from 310 to 620 and 620 to 1,250 µg/mL, respectively. C. sativum essential oil is active in vitro against M. canis and Candida spp. demonstrating good antifungal activity.

  7. Colheita e armazenamento de sementes de coentro Harvesting and storage of coriander seeds

    Directory of Open Access Journals (Sweden)

    Warley Marcos Nascimento

    2006-12-01

    Full Text Available Este trabalho teve por objetivos avaliar a qualidade física e fisiológica de sementes de coentro (Coriandrum sativum colhidas manual e mecanicamente, bem como a qualidade fisiológica e sanitária das sementes inteiras e partidas, armazenadas em diferentes embalagens e condições de ambiente. Sementes de coentro cultivar Verdão foram colhidas manual e mecanicamente. Depois do beneficiamento, foi determinada a porcentagem de pureza, e as sementes foram separadas em sementes inteiras (diaquênios e partidas (aquênios. As sementes foram acondicionadas em embalagens semipermeáveis e impermeáveis e armazenadas durante 12 meses em condições ambientais e em câmara fria (10ºC, 45% UR. A qualidade fisiológica - teste de germinação, primeira contagem, envelhecimento acelerado e emergência das plântulas em casa de vegetação - e sanitária foi avaliada aos 0, 3, 6, 9 e 12 meses de armazenamento. A colheita mecânica apresentou maior porcentagem de sementes partidas. Sementes inteiras, colhidas mecanicamente, apresentaram maior vigor que as sementes inteiras colhidas manualmente. As sementes de coentro conservaram a qualidade até um ano, independentemente das condições de armazenamento. Quanto ao armazenamento em condições ambientais, as sementes devem ser acondicionadas em embalagens impermeáveis. Sementes partidas apresentam potencial para o estabelecimento da cultura de coentro.This study aimed to evaluate the physical and physiological quality of coriander (Coriandrum sativum seeds harvested manually and mechanically, as well as the physiological and sanitary quality of whole and split seeds stored in different containers and storage conditions. 'Verdão' coriander seeds were harvested manually and mechanically. After conditioning, seed purity was determined, and the seeds were separated in two classes: whole seeds (deachenes and split seeds (achenes. Seeds were placed into semipermeable and impermeable containers and stored for

  8. [Contributions to the chemical study of the essential oil isolated from coriander fruits (Sandra cultivar)].

    Science.gov (United States)

    Trifan, Adriana; Aprotosoaie, Ana Clara; Spac, A; Hăncianu, Monica; Miron, Anca; Stănescu, Ursula

    2011-01-01

    Coriandrum sativum L. (Apiaceae) is a well known herb, native to the Mediterranean region, also intensively cultivated in Romania. The essential oil obtained from Coriandri fructus posseses antimicrobial, antioxidant and anxiolytic effects. Many parameters such as genetic and climatic factors or agronomical practices can influence the yield and composition of the volatile fraction. Plant density is an important factor for the microenvironment in coriander field. In order to study the effect of planting density on the yield of the essential oil and its composition, a bifactorial experiment was carried out on coriander plants (Sandra cultivar). The experiment was performed with three plant densities on the row (0, 15 and 20 cm); the distance between plant rows was 12.5, 25 and 50 cm, respectively. So, it resulted nine experimental variants. The essential oils obtained by hydrodistillation from fruits have been characterized using gas chromatography and mass spectroscopy analysis (GC-MS). The highest yield (7.9866 kg/ha) was obtained for the plants spaced at 20 cm in between and 25 cm row spacing. The highest content of monoterpene alcohols (50.96%) was obtained with 25 cm row spacing and plant spaced at 0 cm on the row. The main components in all oils were monoterpene alcohols (40.75% - 50.96%) and monoterpenes (32.43-38.44%). The essential oil of coriander fruits (Sandra cultivar) does not meet the requirements of the European Pharmacopoeia, especially concerning the content in linalool. Nevertheless, the high content in monoterpene alcohols and monoterpenes recommends the use of the essential oil as immunomodulatory, analgesic and antiinflammatory agent in rheumatology and also as an antibacterial and antiviral agent. Consequently, the changes in yield and composition of the essential oil of Sandra coriander should be assesed during several periods of vegetation in order to conclude on its pharmaceutical quality.

  9. Activation of the Kelch-like ECH-associated protein 1 (Keap1)/NF-E2-related factor 2 (Nrf2) pathway through covalent modification of the 2-alkenal group of aliphatic electrophiles in Coriandrum sativum L.

    Science.gov (United States)

    Abiko, Yumi; Mizokawa, Mai; Kumagai, Yoshito

    2014-11-12

    Phytochemicals able to activate the transcription factor NF-E2-related factor 2 (Nrf2) were isolated from an extract of Coriandrum sativum L. (C. sativum) leaves by preparative octadecyl silica column chromatography. Ultraperformance liquid chromatography and liquid chromatography-tandem mass spectrometry analysis of the isolated components after derivatization with 2-diphenylacetyl-1,3-inandione-1-hydrazone and experiments with HepG2 cells revealed that (E)-2-alkenals with different carbon numbers play a role in Nrf2 activation in these cells. Such Nrf2 activation appears to be attributable to S-alkylation of Kelch-like ECH-associated protein 1 (Keap1), the negative regulator for Nrf2, as determined by a biotin-PEAC5-maleimide assay. Interestingly, (E)-2-butenal caused Keap1 modification and Nrf2 activation, whereas butanal did not. These results suggest that (E)-2-alkenals with an α,β-unsaturated aldehyde moiety, which is a common substituent in phytochemicals isolated from C. sativum leaves, activate the Keap1/Nrf2 pathway associated with cellular protection.

  10. Variabilité de la composition chimique des huiles essentielles de Coriandrum sativum L et Pimpinella anisum L au cours de développement végétatif.

    Directory of Open Access Journals (Sweden)

    K. FYAD

    2013-03-01

    Full Text Available Dans cette étude, Nous nous sommes intéressés à quantifier et identifier les huiles essentielles extraites par hydrodistillation des parties aériennes fraîches des deux plantes aromatiques : Coriandrum sativum L et Pimpinella anisum L, aux cours de leur cycle de croissance (avant, pendant et après floraison ; cultivées dans un champ à l’université de Béchar. La récolte a été faite au cours de l'année 2011. Les huiles essentielles obtenues à partir des parties aériennes fraîches durant le cycle végétatif, ont été analysés par CPG.

  11. Arbuscular mycorrhiza differentially affects synthesis of essential oils in coriander and dill.

    Science.gov (United States)

    Rydlová, Jana; Jelínková, Marcela; Dušek, Karel; Dušková, Elena; Vosátka, Miroslav; Püschel, David

    2016-02-01

    Research on the role of arbuscular mycorrhizal fungi (AMF) in the synthesis of essential oils (EOs) by aromatic plants has seldom been conducted in field-relevant conditions, and then, only limited spectra of EO constituents have been analyzed. The effect was investigated of inoculation with AMF on the synthesis of a wide range of EO in two aromatic species, coriander (Coriandrum sativum) and dill (Anethum graveolens), in a garden experiment under outdoor conditions. Plants were grown in 4-l pots filled with soil, which was either γ-irradiated (eliminating native AMF) or left non-sterile (containing native AMF), and inoculated or not with an isolate of Rhizophagus irregularis. AMF inoculation significantly stimulated EO synthesis in both plant species. EO synthesis (total EO and several individual constituents) was increased in dill in all mycorrhizal treatments (containing native and/or inoculated AMF) compared to non-mycorrhizal plants. In contrast, EO concentrations in coriander (total EO and most constituents) were increased only in the treatment combining both inoculated and native AMF. A clear positive effect of AMF on EO synthesis was found for both aromatic plants, which was, however, specific for each plant species and modified by the pool of AMF present in the soil.

  12. Studies on Freeze Drying ofCoriander(Coriandrum Sativum L)%芫荽真空冷冻干燥的研究

    Institute of Scientific and Technical Information of China (English)

    刘达玉; 吕开斌

    2004-01-01

    本文采用真空冷冻干燥技术,探讨了搁板温度、物料重量对芫荽冻干时间、品质及设备生产能力的影响.试验结果表明,搁板设置温度每提高10℃,冻干时间缩短15.68%以上,但温度过高芫荽风味下降,出现干草味;芫荽每增加100g/层,冻干时间延长2.5~3h左右.在温度40℃、芫荽重量400g/层条件下冷冻干燥,冻干时间为12.5h,产品质量优于单纯的真空干燥样品;复水后与新鲜芫荽相对较为接近.

  13. Coriandrum sativum and Melia azedarach

    African Journals Online (AJOL)

    Aghomotsegin

    2015-10-07

    Oct 7, 2015 ... of the gas used and the electron gun were programmed initially. .... antibacterial activity maximum zone formation against E. coli (Table 3). ..... tandem repeat, typing technology, locus information and allele frequency in ...

  14. The effect of Coriandrum sativum seed extract on the learning of newborn mice by electric shock: interaction with caffeine and diazepam

    OpenAIRE

    Moghadamnia, Ali Akbar; Zargar-Nattaj ,Seyed Sadegh; Tayyebi, Pooya; Vahid Zangoori,; Yasaman Moghadamnia; Hasan Roodgari ,; Jorsaraei, Seyed Gholamali

    2011-01-01

    Seyed Sadegh Zargar-Nattaj1, Pooya Tayyebi1, Vahid Zangoori1, Yasaman Moghadamnia4, Hasan Roodgari2, Seyed Gholamali Jorsaraei3, Ali Akbar Moghadamnia11Department of Pharmacology, 2Department of Medical Genetics, 3Department of Anatomical Sciences and Embryology, Babol University of Medical Sciences, Babol, Iran; 4Department of Physics, Alzzahra University, Tehran, IranAbstract: Coriander has been recommended for the relief of pain, anxiety, flatulence, and loss of appetite. In traditional me...

  15. Comparison of Hot Reflux and Ultrasonic-assisted Methods of Extracting Flavonoids from Coriandrum sativum L.%香菜黄酮的乙醇回流与超声波辅助提取工艺对比研究

    Institute of Scientific and Technical Information of China (English)

    刘恒蔚; 潘玉欣; 谈知文

    2011-01-01

    The optimum conditions of extracting flavonoids from dry powder of Coriandrum sativum L. were analyzed by comparing hot reflux method with ultrasonic extracting technology. The results revealed that the extractant concentration and liquid-solid ratio were major impact factors; while the extraction temperature (or ultrasonic amplitude) and extracting timelength were minor factors. According to orthogonal test. the optimal parameters were 25 mL/g of 60% alcohol solution. hot reflux extracting at 80℃ for 3 h; or 25 mL/g of 70% alcohol solution, ultrasonic extracting with an amplitude of 13μm for 45min. The extracting efficiency of hot reflux and presoaking ultrasonic-assisted methods were both as high as 6.914 mg/g.Presoaking treatment could increase ultrasonic extraction efficiency; and ultrasonic-assisted extracting method was suited for industrialization due to its high efficiency and low cost.%为确定香菜(Coriandrum sativum L.)黄酮类物质提取工艺,采用热回流提取法和超声波辅助提取法,研究了不同影响因素对香菜干叶(粉)中黄酮类物质提取量的影响规律,并对经正交试验得到的两种最佳提取工艺进行了比较.研究结果表明,乙醇浓度和液料比是影响提取量的主要因素,其次是提取温度(或超声振幅)和提取时间.最佳工艺参数为60%乙醇,液料比25∶1(V/m,mL∶g),80℃热回流提取3 h;或70%乙醇,液料比25∶1,13μm振幅超声波辅助提取45 min,预浸泡处理可提高超声波辅助提取量,经过预浸泡处理的超声波辅助提取达到与热回流提取一致的提取量,6.914mg/g.

  16. 香菜挥发性次生物质对菜青虫的拒食作用研究%Preliminary study on biological activity of extracts from Coriandrum sativum L.to Pieris rapae L.

    Institute of Scientific and Technical Information of China (English)

    吴利民; 陆宁海; 王国昌; 李卫海; 崔建新

    2012-01-01

    采用水蒸气蒸馏法从香菜(Coriandrum sativum L.)茎叶中提取其挥发性次生物质,测定了香菜提取物在室内对菜青虫的拒食作用.结果表明,香菜提取物对菜青虫3龄幼虫具有较强的拒食活性,且随着提取物浓度的增加,拒食作用增加,药后72 h,香菜提取物对3龄菜青虫拒食作用在稀释倍数为50、100、200、400倍时,拒食率均达到100.0%,稀释800倍时对菜青虫拒食作用相对较弱,拒食率为66.7%.

  17. Effects of Degreasing Treatment with Petroleum Ether on Extracting of Flavonoids from Coriandrum Sativum L.%石油醚除脂处理对香菜总黄酮提取的影响

    Institute of Scientific and Technical Information of China (English)

    刘恒蔚

    2010-01-01

    石油醚除脂处理是目前植物黄酮类物质提取中常用的处理环节.为确定石油醚除脂处理对黄酮类物质提取的影响,以香菜(Coriandrum sativum L.)叶片为材料,进行了石油醚除脂处理的对比研究,并对石油醚除脂时长进行分析.结果表明:对乙醇热回流提取初提物进行石油醚除脂处理后黄酮提取率高于未除脂的对照,具有显著差异;石油醚除脂时间以1.5 h为宜,时长对结果影响较小.在黄酮类物质提取纯化工艺中,应在初提后纯化精制前进行石油醚除脂处理.

  18. Comercialización de semillas importadas de cilantro Coriandrum sativum L. y zapallo Cucurbita moschata durante el periodo enero 1990-agosto 1994

    Directory of Open Access Journals (Sweden)

    Lema Nuñez Rosario M.

    1996-12-01

    Full Text Available

    Of the quantity of imported coriander seeds (3% and of pumpkin (0.2% represent 3.2 % of the total imported a volume of (648.000 kg and 0.79% of the total imported value. Due to a high concentration of these distribution companies in Colombian market (4 or 5 this creates an oligapoly which restricts other producers entering the market, determining the quality, packaging format. The brands are characterized by low prices and reputable quality. The importers fix a high marginal sale (80%, in keeping with the greater percentage (61% of their profits because of low commercialization costs (3% for pumpkin and 5% for coriander. The remaining percentage (14% in coriander and 6% in pumpkin are the detailed marginal output. The preferred variety of pumpkin were Conneticut Field, Candelaria, Sugar, Pie and Golden Delicius, the hybrid seed have not been accepted by the agriculture community.

    Las cantidades importadas de semillas de cilantro (20.393 kg  y de zapallo (1.518 Kg. representaron el 3.2% del volumen total para hortalizas (648.000 kilos y el 0.79% del valor total de dichas importaciones ($8.900 millones. Por la alta concentración del mercado colombiano en 4 ó 5 empresas -productoras y distribuidoras-, constituyen oligopolios, los cuales impiden la entrada de nuevas empresas diferenciando las semillas en forma real (calidad y no real (marca, color del empaque. Las marcas se caracterizan por los precios bajos y calidad. El importador fija márgenes de mercadeo altos (80%, correspondiendo el mayor porcentaje (61% a sus ganancias porque los costos de comercialización son bajos (3% en zapallo y 5% en cilantro. El porcentaje restante (14% en cilantro y 6% en zapallo es el margen del detallista. Las variedades preferidas de zapallo fueron Conneticut Field, Candelaria, Sugar Pie y Golden Delicius; la semilla híbrida no ha sido aceptada por los agricultores. En cilantro se importan el American Long Standing y Coriander (común o de larga

  19. Determination of four volatile components in leaf of Coriandrum sativum L. by GC%GC法测定芫荽茎叶中4种挥发性成分的含量

    Institute of Scientific and Technical Information of China (English)

    姜振元; 李清; 赵龙山; 郑晓娇; 陈晓辉; 毕开顺

    2012-01-01

    Objective To establish a method for determining the contents of linalool,decanal,n-decanol,un-decanal in Coriandrum sativum L. leaf. Methods The sample solution was distilled in vaporizing oil extraction apparatus. With HP-5MSI capillary column(30 m ×0. 25 mm,0. 25 μm),nitrogen as the carrier gas, FID as the detector,the programmed temperature-GC and internal standard(Limonene was used for the internal standard) method were employed to determine the components. Results The results showed that the linear ranges of linalool, decanal, n-decanol and undecanal were 31.50-315.0 mg · L -1 ( r = 0. 999 6), 269. 0 -2 690.0 mg·L-1(r = 0.999 2) ,95.3-953.0 mg·L-1.(r = 0. 999 8) ,58. 1-581.0 mg·L-1 (r =0. 999 7). The average recovery of the method were 102. 0% ( RSD = 1.5% ), 102. 0% ( RSD = 0. 6% ), 101. 9% (RSD = 1. 1% ), 102. 6% ( RSD = 0. 9% ). The average recovery of the method were 102. 0 ( RSD = 1. 5% ) ,102. 0 ( RSD = 0. 6% ), 101. 9 ( RSD = 1.1%), 102. 6 ( RSD = 0. 9% ). Conclusions The results show that this method is steady, accurate and practical which could be used for the determination of linalool, decanal, n-decanol and undecanal in Coriandrum sativum L. leaf.%目的 建立一种芫荽茎叶中芳樟醇、癸醛、正癸醇和十一醛的GC含量测定方法.方法 以挥发油提取器蒸馏制备供试液;HP-5MSI毛细管色谱柱(30m×0.25mm,0.25 μm),以氮气为载气,FID检测器,采用程序升温,内标法测定(柠檬烯为内标物)含量.结果 芳樟醇、癸醛、正癸醇和十一醛进样质量浓度分别在31.5~315.0 mg·L-1(r=0.9996)、269.0~2690.0 mg·L-1(r=0.9997)、95.3 ~953.0 mg·L-1 (r =0.9996)、58.1 ~581.0 mg·L-1 (r =0.9997)内呈良好线性关系,平均回收率分别为102.0(RSD=1.5%)、102.0(RSD=0.6%)、101.9(RSD=1.1%)、102.6(RSD=0.9%).结论 所建立的方法可用于芫荽茎叶中4种挥发性成分含量测定.

  20. Coriander alleviates 2,4-dinitrochlorobenzene-induced contact dermatitis-like skin lesions in mice.

    Science.gov (United States)

    Park, Gunhyuk; Kim, Hyo Geun; Lim, Soonmin; Lee, Wonil; Sim, Yeomoon; Oh, Myung Sook

    2014-08-01

    Contact dermatitis (CD) is a pattern of inflammatory responses in the skin that occurs through contact with external factors. The clinical picture is a polymorphic pattern of skin inflammation characterized by a wide range of clinical features, including itching, redness, scaling, and erythema. Coriandrum sativum L. (CS), commonly known as coriander, is a member of the Apiaceae family and is cultivated throughout the world for its nutritional and culinary values. Linoleic acid and linolenic acid in CS have various pharmacological activities. However, no study of the inhibitory effects of CS on CD has been reported. In this study, we demonstrated the protective effect of CS against 2,4-dinitrochlorobenzene-induced CD-like skin lesions. CS, at doses of 0.5-1%, applied to the dorsal skin inhibited the development of CD-like skin lesions. Moreover, the Th2-mediated inflammatory cytokines, immunoglobulin E, tumor necrosis factor-α, interferon-γ, interleukin (IL)-1, IL-4, and IL-13, were significantly reduced. In addition, CS increased the levels of total glutathione and heme oxygenase-1 protein. Thus, CS can inhibit the development of CD-like skin lesions in mice by regulating immune mediators and may be an effective alternative therapy for contact diseases.

  1. Effect of foliar application of urea and planofix on the foliage yield of coriander

    Directory of Open Access Journals (Sweden)

    Abdur Rakib

    2015-06-01

    Full Text Available An experiment on coriander (Coriandrum sativum L. was conducted at the experimental field of Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU, Gazipur during November 2012 to April 2013 to find out the suitable foliar doses and application frequency of urea and planofix (NAA. The experiment was laid out in factorial randomized complete block design with three replications. The treatment consisted of six foliar dozes viz. T1 (Tap water as control, T2 (0.10 % urea, T3 (0.25 % urea, T4 (0.40 % urea, T5 (5 ppm planofix and T6 (10 ppm planofix and three application frequencies viz. F1 [20 days after sowing (20 DAS], F2 (30 DAS and F3 (20 and 30 DAS. Maximum foliage yield (6.94 t/ha was recorded in 10 ppm planofix coupled with it’s twice application at 20 and 30 DAS which was closely followed the foliage yield (6.33 t/ha by 5 ppm planofix with the same application frequency. The foliage yield was increased with the increase in urea concentration. The highest foliage yield (5.37 t/ha was also recorded from twice application of urea and planofix at 20 and 30 DAS, respectively. Planofix 10 ppm with its twice application at 20 and 30 DAS gave the highest benefit-cost of ration 2.51.

  2. Optimization of ultrasound-assisted extraction of polyphenolic compounds from coriander seeds using response surface methodology

    Directory of Open Access Journals (Sweden)

    Zeković Zoran P.

    2016-01-01

    Full Text Available Coriandrum sativum L. (coriander seeds (CS were used for preparation of extracts with high content of biologically active compounds. In order to optimize ultrasoundassisted extraction process, three levels and three variables of Box-Behnken experimental design (BBD in combination with response surface methodology (RSM were applied, yielding maximized total phenolics (TP and flavonoids (TF content and antioxidant activity (IC50 and EC50 values. Independent variables were temperature (40-80oC, extraction time (40-80 min and ultrasonic power (96-216 W. Experimental results were fitted to a second-order polynomial model with multiple regression, while the analysis of variance (ANOVA was employed to assess the model fitness and determine optimal conditions for TP (79.60oC, 49.20 min, 96.69 W, TF (79.40oC, 43.60 min, 216.00 W, IC50 (80.00oC, 60.40 min, 216.00 W and EC50 (78.40oC, 68.60 min, 214.80 W. On the basis of the obtained mathematical models, three-dimensional surface plots were generated. The predicted values for TP, TF, IC50 and EC50 were: 382.68 mg GAE/100 g CS, 216 mg CE/100 g CS, 0.03764 mg/mL and 0.1425 mg/mL, respectively. [Projekat Ministarstva nauke Republike Srbije, br. TR31013

  3. The kinetics of the swelling process and the release mechanisms of Coriandrum sativum L. essential oil from chitosan/alginate/inulin microcapsules.

    Science.gov (United States)

    Dima, Cristian; Pătraşcu, Livia; Cantaragiu, Alina; Alexe, Petru; Dima, Ştefan

    2016-03-15

    The encapsulation by spray drying method of coriander essential oil (CEO) in various materials (chitosan, alginate, chitosan/alginate, chitosan/inulin) was studied. The viscoelastic properties of the oil-in-water (O/W) emulsions and the characteristics of CEO-loaded microcapsules like morphology, moisture, wettability, solubility, flowability properties, swelling and release mechanisms were investigated. The chitosan microcapsules had a brain-like structure while the alginate and chitosan/alginate microcapsules are spherical with a smooth surface. The Compressibility Index (CI=29.09-32.25%) and Hausner Ratio (HR=1.38-1.44) values showed that all the microcapsules prepared correspond to the "poor" flowability powders group. The chitosan microcapsules exhibited the maximum release rate at pH 2.5 while the alginate microcapsules exhibited the maximum release rate at pH 6.5. Kinetics and mechanism of CEO release were studied using various mathematical models such as, zero order, first order, Higuchi model and Peppas model. The diffusional exponent (n) values of Peppas equation explains a non Fickian transport mechanism and diffusion or diffusion-swelling controlled process.

  4. Change of fruit anatomical structure of Coriandrum sativum (Apiaceae) during fruit development process%伞形科植物芫荽果实发育过程中的解剖结构变化

    Institute of Scientific and Technical Information of China (English)

    卞桂兰; 刘启新; 宋春凤

    2014-01-01

    采用常规石蜡切片法对伞形科(Apiaceae)芹亚科(Apioideae Drude)芫荽属(Coriandrum Linn.)种类芫荽(Coriandrum sativum Linn.)花后幼果发育阶段和果熟发育阶段的果实横切面解剖结构及其变化进行了观察和比较.观察结果表明:芫荽果实的幼果和果熟发育阶段均分为前期、中期和后期3个时期,不同时期果实横切面的形状和大小、果壁厚度和结构以及合生面、果棱、维管束、油管、胚乳和结晶等特征均有明显变化.在芫荽果实发育过程中,果实横切面由近椭圆形逐渐变为近圆形,且分生果长度从1 630 μm增加至3 290 μm、宽度从860 μm增加至1 580 μm.合生面长度与分生果长度相等,但合生面逐渐分离,成熟的2个分生果仅由心皮柄维管束和合生面两端的外果壁及中果壁最外侧数层细胞相连,从外形看2个分生果不分离.果壁由薄变厚、再由厚变薄,明显分为4个层次;成熟果实的中果壁大部分由木化细胞构成.果棱可分为主棱和次棱2种,且均不发达;次棱呈角状突起、主棱呈波状弯曲,次棱较主棱明显.维管束包括果壁维管束、心皮柄维管束和种脊维管束3类,其中,果壁维管束不发达且散布于中果壁木化细胞层中.油管仅存在于合生面并被逐渐压扁,而果壁油管消失并转变成大空腔.胚乳逐渐膨大,其形状由元宝形逐渐转变为船形或肾形.此外,在幼果发育阶段,中果壁薄壁细胞、合生面和胚乳细胞中或多或少都含有结晶,胚乳细胞中还含有大量糊粉粒结晶;但在果熟发育阶段结晶消失,仅胚乳细胞中存有糊粉粒结晶.分析结果显示:芫荽果实属隐性双悬果类型且果棱属微果棱型,其部分解剖结构与伞形科芹亚科多数种类的果实有差异.

  5. Coriander seed oil methyl esters as biodiesel fuel: Unique fatty acid composition and excellent oxidative stability

    Energy Technology Data Exchange (ETDEWEB)

    Moser, Bryan R.; Vaughn, Steven F. [United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N. University St, Peoria, IL 61604 (United States)

    2010-04-15

    Coriander (Coriandrum sativum L.) seed oil methyl esters were prepared and evaluated as an alternative biodiesel fuel and contained an unusual fatty acid hitherto unreported as the principle component in biodiesel fuels: petroselinic (6Z-octadecenoic; 68.5 wt%) acid. Most of the remaining fatty acid profile consisted of common 18 carbon constituents such as linoleic (9Z,12Z-octadeca-dienoic; 13.0 wt%), oleic (9Z-octadecenoic; 7.6 wt%) and stearic (octadecanoic; 3.1 wt%) acids. A standard transesterification procedure with methanol and sodium methoxide catalyst was used to provide C. sativum oil methyl esters (CSME). Acid-catalyzed pretreatment was necessary beforehand to reduce the acid value of the oil from 2.66 to 0.47 mg g{sup -1}. The derived cetane number, kinematic viscosity, and oxidative stability (Rancimat method) of CSME was 53.3, 4.21 mm{sup 2} s{sup -1} (40 C), and 14.6 h (110 C). The cold filter plugging and pour points were -15 C and -19 C, respectively. Other properties such as acid value, free and total glycerol content, iodine value, as well as sulfur and phosphorous contents were acceptable according to the biodiesel standards ASTM D6751 and EN 14214. Also reported are lubricity, heat of combustion, and Gardner color, along with a comparison of CSME to soybean oil methyl esters (SME). CSME exhibited higher oxidative stability, superior low temperature properties, and lower iodine value than SME. In summary, CSME has excellent fuel properties as a result of its unique fatty acid composition. (author)

  6. Chemical Composition and Antimicrobial Activity of Essential Oils from Black Pepper, Cumin, Coriander and Cardamom Against Some Pathogenic Microorganisms

    Directory of Open Access Journals (Sweden)

    Teneva Desislava

    2016-12-01

    Full Text Available Four popular spices black pepper (Piper nigrum L., cumin (Cuminum cyminum L., coriander (Coriandrum sativum L. and cardamom (Elettaria cardamomum were analyzed for their oil composition by GC-MS. Thirty compounds were identified in the black pepper oil and the main components were β-caryophyllene (20.225 %, sabinene (18.054 %, limonene (16.924 %, α-pinene (9.171 % and α-phellandrene (5.968 %. Twenty five compounds were identified in the cumin oil – cuminaldehyde (30.834 %, 3-caren-10-al (17.223 %, β-pinene (14.837 %, γ–terpinene (11.928 %, 2-caren-10-al (8.228 % and pcymene (6.429 %. Twenty nine compounds were identified in the coriander oil – β-linalool (58.141 %, α-pinene (8.731 %, γ-terpinene (6.347 % and p-cymene (5.227 %. Twenty nine compounds were identified in the cardamom oil – α-terpinyl acetate (39.032 %, eucalyptol (31.534 %, β-linalool (4.829 %, sabinene (4.308 % and α-terpineol (4.127 %. The antimicrobial activity of essential oils against pathogenic (Escherichia coli ATCC 25922, Escherichia coli ATCC 8739, Salmonella sp. (clinical isolate, Staphylococcus aureus ATCC 6538P, Proteus vulgaris G microorganisms by disc-diffusion method was examined. Gram-positive bacteria were more sensitive to the oils (inhibition zones being between 8 and 12.5 mm and the minimum inhibitory concentration was more than 600 ppm; Gram-negative bacteria were less sensitive. The obtained essential oils are suitable for use as biopreservative agents.

  7. Burdur İli Anason (Pimpinella anisum L., Kişniş (Coriandrum sativum L. ve Rezene (Foeniculum vulgare Mill. Agro-Ekosistemlerinde Böcek Biyolojik Çeşitliliklerinin Belirlenmesi

    Directory of Open Access Journals (Sweden)

    Gökhan AYDIN

    2014-02-01

    Full Text Available Bu çalışma, Burdur ilinde kültürü yapılan önemli tıbbi ve aromatik bitkilerden pestisit uygulaması yapılmayan ve periyodik olarak münavebe yöntemi uygulanan anason (Pimpinella anisum L., kişniş (Coriandrum sativum L. ve rezene (Foeniculum vulgare Mill. agro-ekosistemlerinde böcek biyolojik çeşitliliklerinin araştırılması amacı ile böcek aktivitesinin yoğun olduğu 2009 yılı nisan ve eylül ayları arasında yapılmıştır. Çukur tuzak örnekleme yönteminin uygulandığı habitatlardaki böcek biyolojik çeşitlilik değerleri Shannon-Wiener ve Simpson çeşitlilik, Simpson dominantlık, Shannon Populasyon yoğunluk ilişkisi parametreleri kullanılarak hesaplanmıştır. Buna göre hesaplanan biyolojik çeşitlilik indekslerinde rezene agro-ekosisteminde hem Shannon-Wiener hem de Simpson çeşitlilik indeks değerleri sırası ile 2.5838 ve 0.8742 ile diğer agro-ekosistemlere göre en yüksek bulunmuştur. Simpson dominantlık parametre sonucuna göre ise 0.2025 değeri ile kişniş agro-ekosistemi, anason ve rezene agro-ekosistemlerine göre dominantlığın en yüksek olduğu tarım alanı olarak belirlenmiştir. Habitatlardaki populasyon yoğunluk ilişkisi incelendiğinde Shannon Evenness değeri anason agro-ekosisteminde en dengeli olarak bulunmuş ve 0.7333 olarak hesaplanmıştır. Bu sonuçlara göre pestisit uygulaması yapılmayan ve periyodik olarak münavebe yöntemi uygulanan agro-ekosistemlerdeki böcek türleri ve birey sayılarından elde edilen çeşitlilik değerlerinin doğal habitatlara benzerlik gösterdiği ortaya çıkartılmıştır. Anahtar kelimeler: Tür Zenginliği, Shannon-Wiener Çeşitlilik, Simpson Dominantlık, Populasyon Yoğunluk İlişkisi, Çukur Tuzak

  8. VOLATILE CONSTITUENTS OF THE FRUIT OF CORIANDRUM SATIVUML. FROM ISFAHAN

    Directory of Open Access Journals (Sweden)

    ALIREZA GHANNADI

    1999-10-01

    Full Text Available The essential oil of the fruits of Coriandrum sativum L. which is cultivated in Isfahan was isolated by steam distillation and analyzed by TLC, GC, GC/MS and ^-NMR. Eight compounds representing 95.3% of the total components were characterized. Linalool (56.2%, y- terpinene (12.0% and 5-3-carene (9.7% were the major constituents of the oil which were obtained in 0.68% (V/W yield.

  9. 北方大棚绿芦笋套种香菜栽培技术%Greenhouse Intercropping of Green Asparagus (Asparagus officinalis Linn) and Coriander (Coriandrum sativum L.)

    Institute of Scientific and Technical Information of China (English)

    郑毅; 李玉环; 李哲; 徐海玉; 凤桐

    2016-01-01

    在北方大棚种植模式单一,套种模式不常见,北方大棚绿芦笋种植同时套种香菜,既可以增加蔬菜种类,又可以提高复种指数,还能增加芦笋种植户的收入.该文通过大棚绿芦笋套种香菜栽培技术的介绍,为北方绿芦笋大棚栽培大面积推广提供有力技术支撑,打破大棚种植单一的模式,对种植模式起到引领作用.

  10. 芫荽种质资源形态标记遗传多样性分析%Genetic Diversity Analysis of Coriander (Coriandrum sativum L.)Using Morphological Markers

    Institute of Scientific and Technical Information of China (English)

    李小梅; 杜洁; 张立微; 张景涛

    2015-01-01

    为合理利用芫荽种质资源,该研究以96份芫荽种质为材料,采用形态学标记评价芫荽种质的遗传多样性,并对芫荽19个生物学性状进行分析.结果表明:抽薹天数与始花期、终花期、收获天数、基生叶数、基生叶长、株高、植株生叶状态、分枝数均呈极显著正相关,基生叶数与最长基生叶长、基生叶形、植株有叶状态、植株分枝数均呈极显著正相关,最长基生叶长与株高呈极显著正相关,抽薹天数及基生叶数均与千粒重呈负相关.在遗传距离6.40处将96份种质分为5类.

  11. Coriander leaf extract exerts antioxidant activity and protects against UVB-induced photoaging of skin by regulation of procollagen type I and MMP-1 expression.

    Science.gov (United States)

    Hwang, Eunson; Lee, Do-Gyeong; Park, Sin Hee; Oh, Myung Sook; Kim, Sun Yeou

    2014-09-01

    Ultraviolet (UV) radiation causes photodamage to the skin, which, in turn, leads to depletion of the dermal extracellular matrix and chronic alterations in skin structure. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-1 (MMP-1) activity. Coriandrum sativum L. (coriander leaf, cilantro; CS) has been used as a herbal medicine for the treatment of diabetes, hyperlipidemia, liver disease, and cancer. In this study, we examined whether CS ethanol extract (CSE) has protective effects against UVB-induced skin photoaging in normal human dermal fibroblasts (NHDF) in vitro and in the skin of hairless mice in vivo. The main component of CSE, linolenic acid, was determined by gas chromatography-mass spectroscopy. We measured the cellular levels of procollagen type I and MMP-1 using ELISA in NHDF cells after UVB irradiation. NHDF cells that were treated with CSE after UVB irradiation exhibited higher procollagen type I production and lower levels of MMP-1 than untreated cells. We found that the activity of transcription factor activator protein-1 (AP-1) was also inhibited by CSE treatment. We measured the epidermal thickness, dermal collagen fiber density, and procollagen type I and MMP-1 levels in photo-aged mouse skin in vivo using histological staining and western blot analysis. Our results showed that CSE-treated mice had thinner epidermal layers and denser dermal collagen fibers than untreated mice. On a molecular level, it was further confirmed that CSE-treated mice had lower MMP-1 levels and higher procollagen type I levels than untreated mice. Our results support the potential of C. sativum L. to prevent skin photoaging.

  12. КОМПОНЕНТНЫЙ СОСТАВ ЭФИРНОГО МАСЛА ПЛОДОВ CORIANDRUM SATIVUM, ПРОИЗРАСТАЮЩЕГО В СИБИРСКОМ РЕГИОНЕ

    OpenAIRE

    ЗЫКОВА ИРИНА ДЕМЕНТЬЕВНА; ПУТИНЦЕВА АЛЕНА АЛЕКСАНДРОВНА; ЕФРЕМОВ АЛЕКСАНДР АЛЕКСЕЕВИЧ

    2014-01-01

    Изучен компонентный состав эфирного масла плодов Coriandrum sativum, произрастающего на территории Красноярского края. Методом хромато-масс-спектрометрии установлено содержание в масле более 30 компонентов, 29 из которых идентифицированы. Проведен сравнительный анализ компонентного состава эфирного масла Coriandrum sativum из разных мест произрастания....

  13. Bactericidal activity of black pepper, bay leaf, aniseed and coriander against oral isolates.

    Science.gov (United States)

    Chaudhry, Nazia Masood Ahmed; Tariq, Perween

    2006-07-01

    Present investigation focused on antibacterial potential of aqueous decoction of black pepper (Piper nigrum L.), bay leaf (Laurus nobilis L.), aniseed (Pimpinella anisum L.), and coriander (Coriandum sativum L.) against 176 bacterial isolates belonging to 12 different genera of bacterial population isolated from oral cavity of 200 individuals. The disc diffusion technique was employed. Overall aqueous decoction of black pepper was the most bacterial-toxic exhibited 75% antibacterial activity as compared to aqueous decoction of bay leaf (53.4%) and aqueous decoction of aniseed (18.1%), at the concentration of 10 ml/disc. The aqueous decoction of coriander did not show any antibacterial effect against tested bacterial isolates.

  14. Prevalence of Strongyloides stercoralis contaminating Coriandrum obtained from vendors in Karimnagar

    Directory of Open Access Journals (Sweden)

    Krishnappa Muniswamappa

    2012-01-01

    Full Text Available Helminthic contamination of fresh vegetables is common. 116 samples of Coriandrum sativum was processed by Baermann funnel method for recovering Strongyloides stercoralis larvae which were identified by their morphology. The prevalence rate of contamination with larvae was found out. Out of 116 samples, 73 (63% samples were harboring the parasites and 43 (37% samples had no larvae. Among 73 positive samples, 25 samples contained rhabdatiform larvae, 12 contained filariform larvae, and 36 contained both types of larvae. 63% prevalence rate of contamination of Coriandrum sativum in Karimnagar is the definite risk factor for acquiring infection by farm workers, handlers, transporters, and consumers of this vegetable. This high prevalence of larvae acts as an environmental source of the parasite; especially the infective filariform forms constituting 65% of the contaminated samples. Larvae can spread from the source through direct contact while handling the vegetable, necessitating estimation of asymptomatically infected persons and burden of the parasitic infestation.

  15. Evaluation of Biomass Yield and Water Treatment in Two Aquaponic Systems Using the Dynamic Root Floating Technique (DRF)

    National Research Council Canada - National Science Library

    Silva, Laura; Gasca-Leyva, Eucario; Escalante, Edgardo; Fitzsimmons, Kevin; Lozano, David

    2015-01-01

    ... (Oreochromis niloticus). The hydroponic culture in one treatment (PAK) was pak choy (Brassica chinensis,) and in the other (COR) coriander (Coriandrum sativum). Initial and final weights were determined for the fish culture...

  16. Biodiesel From Alternative Oilseed Feedstocks: Production and Properties

    Science.gov (United States)

    Fatty acid methyl esters were prepared and evaluated as potential biodiesel fuels from several alternative oilseed feedstocks, which included camelina (Camelina sativa L.), coriander (Coriandrum sativum L.), field mustard (Brassica juncea L.), field pennycress (Thlaspi arvense L.), and meadowfoam (L...

  17. Classification of specialty seed meals from NIR reflectance spectra

    Science.gov (United States)

    Near infrared reflectance spectroscopy was used to identify alternative seed meals proposed for food and feed formulations. Spectra were collected from cold pressed Camelina (Camelina sativa), Coriander (Coriandrum sativum), and Pennycress (Thlaspi arvense) meals. Additional spectra were collected ...

  18. Survey of alternative feedstocks for biodiesel production

    Science.gov (United States)

    Summarized will be results obtained from the production of biodiesel from several alternative feedstocks with promising agronomic characteristics. Such feedstocks include camelina (Camelina sativa L.), coriander (Coriandrum sativum L.), field pennycress (Thlaspi arvense L.), and meadowfoam (Limnanth...

  19. EKSTRAKSI MINYAK KETUMBAR (Coriander Oil DENGAN PELARUT ETANOL DAN n-HEKSANA

    Directory of Open Access Journals (Sweden)

    Prima Astuti Handayani

    2013-05-01

    Full Text Available Indonesia memiliki banyak sumber daya alam, diantaranya minyak atsiri. Salah satu sumber daya alam yang potensial adalah minyak biji ketumbar (coriandrum oil. Kandungan terbesar dalam minyak ketumbar adalah senyawa linalool yang dapat dimanfaatkan sebagai bahan baku parfum, farmasi, aroma makanan dan minuman, sabun mandi, bahan dasar lilin, sabun cuci, sintesis vitamin E dan pestisida maupun insektida. Tujuan dari percobaan ini adalah untuk mempelajari pengaruh penggunaan pelarut etanol dan n-heksana terhadap rendemen minyak ketumbar yang dihasilkan serta senyawa kimia yang terdapat dalam minyak ketumbar. Ekstraksi minyak ketumbar dengan pelarut etanol dan n-heksana menggunakan alat ekstraktor soxhlet. Biji ketumbar yang tua dan kering dihancurkan kemudian dibungkus kertas saring dan dimasukan dalam ekstraktor soxhlet. Temperatur proses ekstraksi sesuai dengan titik didih dari pelarut yang digunakan. Ekstraksi berakhir jika warna pelarut dalam ekstraktor seperti warna pelarut semula. Filtrat yang diperoleh kemudian di recovery dengan ekstraktor soxhlet untuk memisahkan minyak atsiri dari pelarutnya. Minyak ketumbar kemudian di analisis dengan uji GC-MS untuk mengetahui senyawa kimia yang terkandung dalam minyak tersebut. Dari hasil percobaan diperoleh bahwa rendemen minyak ketumbar dengan pelarut etanol sebesar 1,17% dengan kadar linalool sebesar 57,13%, sedangkan dengan pelarut n-heksana diperoleh rendemen minyak ketumbar sebesar 0,84% dengan kadar linalool sebesar 47,25%. Indonesia has many natural resources, such as the essential oils. One of the potential re-sources is the coriander seed oil (coriandrum oil. The greatest content in coriander oil is linalool compounds that can be used as raw materials of perfumes, pharmaceuticals, food and beverage scent, soap, basic materials for candles, laundry soap, synthetic vitamin E and pesticides as well as insecticide. The purpose of this experiment was to study the effect of the use of ethanol

  20. Desempenho agronômico e divergência genética de genótipos de coentro Agronomic performance and genetic divergence of coriander genotypes

    Directory of Open Access Journals (Sweden)

    Cândida Hermínia de Magalhães Bertini

    2010-09-01

    Full Text Available Cinco genótipos de Coriandrum sativum, procedentes de diferentes regiões do Estado do Ceará e duas cultivares comerciais, foram avaliados objetivando-se identificar genótipos com potencial agronômico para serem utilizados em programa de melhoramento genético por meio de análise de desempenho e divergência genética. O trabalho foi conduzido no período de setembro a dezembro de 2007 no município de Fortaleza-CE. O delineamento utilizado foi o aleatorizado em blocos com três repetições, onde os genótipos e cultivares foram considerados tratamentos. Na análise de desempenho verificou-se diferença entre as médias dos genótipos avaliados para as características: altura da planta, diâmetro do colo, surgimento da primeira inflorescência, antese, média de umbeletes/umbela, início e término do amadurecimento dos frutos. Entretanto, para as características agronômicas, número de folhas aproveitáveis e peso de cem frutos, não se verificou diferença entre as médias dos genótipos avaliados em relação às cultivares comerciais. Quanto à divergência, o genótipo 1, proveniente da região litorânea de Caucaia, foi o mais divergente, podendo ser usado em cruzamentos com os demais genótipos para a obtenção de populações segregantes. Os genótipos mais similares foram o Verdão-SF177 e o genótipo proveniente de Juazeiro. Os resultados do agrupamento não mostraram relação com as diferentes localizações geográficas dos genótipos avaliados.Five genotypes of coriander (Coriandrum sativum from different regions of Ceara-Brazil and two cultivars were assessed with the objective to identify genotypes with agronomic potential for use in breeding program using performance analysis and genetic divergence. The study was conducted during the period of September to December, 2007 in Fortaleza - Ceará. It was used a randomized blocks design with three replicates where the genotypes and cultivars were considered treatments. In

  1. The Effect of Coriander Sativum Seeds on Performance in Japanese ...

    African Journals Online (AJOL)

    Administrator

    of antibiotics-resistant bacteria (Schwarz et al., 2001; Lee et al., 2004). Consequently, the use of ..... a.b.c Means within rows with different superscripts differ significantly (P < 0.05); s.e.m. – standard error of ... cerevisiae. Similarly, Çabuk et al.

  2. Qualidade fisiológica de sementes de coentro [Coriandrum sativum (L.] Physiologic quality of cilantro seeds [Coriandrum sativum (L.

    Directory of Open Access Journals (Sweden)

    M.F.S. Pereira

    2011-01-01

    Full Text Available A qualidade das sementes esta relacionada de forma direta ao seu potencial fisiológico, representado pela germinação e/ou vigor, expressando sua capacidade de originar plântulas normais. O objetivo do estudo foi a análise dos efeitos da temperatura e do tempo de exposição ao teste de envelhecimento acelerado sobre os parâmetros: germinação, germinação na primeira contagem, emergência, Índice de velocidade de emergência, comprimento de parte aérea e massa seca. O ensaio foi conduzido no delineamento inteiramente casualizado, com quatro repetições. As sementes foram submetidas à dois métodos de envelhecimento acelerado: o tradicional com água destilada e outro com solução saturada de 40 g NaCl 100 mL-1 de água, em duas temperaturas (38 ºC e 41 ºC e em três períodos de exposição (48, 72 e 96 horas. Melhor se pode verificar o potencial fisiológico das sementes de coentro, quando submetido às condições estressantes: na temperatura de 41 °C com o período de exposição de 96 h (Teste envelhecimento acelerado tradicional e na temperatura de 41 ºC com período de exposição de 48 h (com solução saturada. Os testes de germinação e primeira contagem evidenciaram diferenças na qualidade fisiológica inicial das sementes em função dos tratamentos aplicados.The quality quality of the seeds this related of direct form to it physiologic potential, acted by the germination and/or energy, expressing it capacity to originate normal plantules. The objective of the study was the analysis of the effects of the temperature and of the time of exhibition to the test of accelerated aging on the parameters: germination, germination in the first counting, emergency, Index of emergency speed, length of aerial part and mass dries. The rehearsal was driven in the delineamento entirely casualizado, with four repetitions. The seeds were submitted to two methods of accelerated aging: the traditional with distilled water and other with saturated solution of 40 g NaCl 100 mL-1 of water, in two temperatures (38 ºC and 41 ºC and in three exhibition periods (48, 72 and 96 hours. Better the physiologic potential of the cilantro seeds can be verified, when submitted to the stressful conditions: in the temperature of 41 °C with the period of exhibition of 96 h (it Tests traditional aging and in the temperature of 41 °C with period of exhibition of 48 h (with saturated solution. The germination tests and first counting evidenced differences in the quality physiologic initial of the seeds in function of the applied treatments.

  3. The effect of Coriander cream on healing of superficial second degree burn wound

    Directory of Open Access Journals (Sweden)

    Abolfazl Abbas Zadeh

    2015-12-01

    Full Text Available Background: Coriander with the binominal name of Corianda Sativum, is one of the oldest medicinal plants ever known to man. Anti-inflammatory, anti-microbial, and anti-fungal effects of its oil has been mentioned in numerous studies. This study examines the impact of coriander cream on wound healing of the second-degree singe burn. Methods: In this experimental study which was performed in the animal lab of the Hazrat Fatemeh Hospital in Tehran, 48 adult male rats with an approximate weight of 250-300 grams, with deep burns of 2 cm 4×2 dimensions were prepared and divided into 4 groups of 12. We used silver sulfadiazine cream, alpha ointment, coriander cream and vaseline gauze (control group dressings in burn wound of the groups 1 to 4 respectively. At the end of the study (30 days, rats were euthanized with a high dose of thiopental and the wounds were evaluated on days 10 and 17 with a punch biopsy. Samples were fixed with 10% formalin on histopathology slide using haematoxylin and eosin (H&E staining (to assess and determine the presence of inflammatory cells. The amount of fibrin and collagen at the site were evaluated using a software program ImageJ, version 1.45 (National Institutes of Health, Bethesda, Maryland, USA. Results: The mean of wound surface area in the first photography was no significant (P= 0. 135. The rate of wound healing in alpha ointment and coriander cream had better outcomes than either of the other two groups (P= 0.000. The healing of the wound in silver sulfadiazine group was significantly less than other groups. Pathology results showed a statistically significant difference between the four groups (coriander, alpha, SSD and control, based on the Kruskal-Wallis test. These relate to (1 polymorphonuclear in the first (P= 0.032 and the second series (P= 0.003, (2 Angiogenesis in the second series (P= 0.004. (3 Fibrosis in the first series (P= 0.024 and the second series (P= 0.000. Conclusion: The results of this study

  4. Caracterização morfológica de genótipos de coentro Morphological characterization of coriander genotypes

    Directory of Open Access Journals (Sweden)

    Roberto de A Melo

    2009-09-01

    Full Text Available Poucas cultivares de coentro (Coriandrum sativum L. estão disponíveis aos produtores e, em algumas regiões, cultivam-se genótipos de origem desconhecida, mantidos pelos próprios hortelãos. Para conservar as cultivares tradicionais é necessário não só coletá-las e conservá-las, mas também caracterizá-las. Dessa forma, este trabalho teve por objetivo determinar caracteres morfológicos para diferenciar genótipos de coentro no processo de registro de cultivares. Foram realizados dois experimentos, ambos em blocos casualizados, com cinco repetições. As cultivares Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá e Verdão, além da linhagem HTV-9299 foram analisadas para caracteres de fruto, plântula, folhas, folíolos e flores. Os dados obtidos foram submetidos à análise de variância univariada. A cultivar Palmeira apresentou frutos de maior tamanho e massa. Já para antocianina a cultivar Americano apresentou menor intensidade, ou praticamente ausência do pigmento tanto nas plântulas quanto nas plantas, ao contrário da cultivar Tabocas que apresentou maior intensidade. As cultivares Americano, Tabocas, Palmeira, Asteca, Verdão e Tabocas e a linhagem HTV-9299 possuem maior número de folhas basais, no entanto, as cultivares que produziram maior quantidade de massa por planta foram Tabocas, Palmeira, Verdão, HTV-9299 e Tapacurá. A cultivar Americano possui folíolo verde claro pouco amarelado, a linhagem HTV-9299 folíolo verde e a cultivar Tabocas folíolo verde escuro. Os descritores morfológicos com maior poder discriminatório para o grupo de dez cultivares avaliadas foram tamanho e massa de fruto; antocianina na plântula e planta; número de folhas basais; comprimento da quinta folha; tamanho e coloração de folíolo; número de dias para pendoamento e abertura da primeira flor e massa da planta fresca.Few coriander cultivars (Coriandrum sativum L. are available to producers and, in some

  5. Applying Molecular Markers in Coriander Populations with Diverse Geographical Origins

    Science.gov (United States)

    Relationships between patterns of genetic diversity and geographical origins were studied in coriander by using amplified fragment length polymorphisms (AFLP) to survey coriander accessions. In 2005, 60 coriander accessions from 28 countries, from the USDA-ARS North Central Regional Plant Introduct...

  6. Herbicidal activity of volatiles from coriander, winter savory, cotton lavender, and thyme isolated by hydrodistillation and supercritical fluid extraction.

    Science.gov (United States)

    Grosso, Clara; Coelho, José A; Urieta, José S; Palavra, António M F; Barroso, José G

    2010-10-27

    The volatiles from Coriandrum sativum L., Satureja montana L., Santolina chamaecyparissus L., and Thymus vulgaris L. were isolated by hydrodistillation (essential oil) and supercritical fluid extraction (volatile oil). Their effect on seed germination and root and shoot growth of the surviving seedlings of four crops ( Zea mays L., Triticum durum L., Pisum sativum L., and Lactuca sativa L.) and two weeds ( Portulaca oleracea L. and Vicia sativa L.) was investigated and compared with those of two synthetic herbicides, Agrocide and Prowl. The volatile oils of thyme and cotton lavender seemed to be promising alternatives to the synthetic herbicides because they were the least injurious to the crop species. The essential oil of winter savory, on the other hand, affected both crop and weeds and can be appropriate for uncultivated fields.

  7. Environ: E00551 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00551 Coriandrum sativum herb Crude drug Coriandrum sativum [TAX:4047] Apiaceae (c...arrot family) Coriandrum sativum herb Crude drugs [BR:br08305] Dicot plants: asterids Apiaceae (carrot family) E00551 Coriandrum sativum herb ...

  8. Volatile Constituents of Romanian Coriander Fruit

    Directory of Open Access Journals (Sweden)

    Anna Tsagkli

    2012-01-01

    Full Text Available The essential oils of Romanian coriander fruits ( cultivar “Sandra” were obtained by hydrodistillation and analyzed by means of GC and GC-MS. Sixty compounds were identified in the total essential oils. Monoterpenes were the most dominant class of compounds, with linalool (48.4-54.3% being the major component. Other significant compounds were γ-terpinene (9.2-12.1%, α-pinene (5.5-9.3% and limonene (4.7-6.3%.

  9. MICROENCAPSULATION OF CORIANDER OIL USING COMPLEX COACERVATION METHOD

    Directory of Open Access Journals (Sweden)

    CRISTIAN DIMA

    2014-03-01

    Full Text Available In the present study, the gelatin/gum Arabic/chitosan microcapsules encapsulating coriander oil were prepared by complex coacervation using glutaric aldehide and transglutaminase as hardening agents. The effects of wall materials, core/wall ratio, particle size, cross-linking agents, dispersing medium and temperature on the release of coacervate microcapsule were investigated. The antioxidative properties and antibacterial activity of free and microencapsulated coriander oil were examined. In the case of DPPH assay, the IC50 values of coriander oil free and coacervat microcapsule was comparables. Addition of chitosan in wall materials did not interfere with the antioxidant activity of coriander oil, but it improves the antibacterial activity of system. Coriander oil extraction by hydrodistillation revealed the influence of particle size on extraction yield. It increases with the decreasing of particle size resulting in maximum efficiency 0.836% for particles of 500 µm, 0.753% for those of 630 µm, and 0.704% for the size of 710 μm.

  10. Optimization of RAPD Reaction Conditions for Coriander sativum%芫荽RAPD体系优化

    Institute of Scientific and Technical Information of China (English)

    李小梅; 栾杰; 张景涛; 张丽茁; 张立微; 董守坤

    2011-01-01

    以芜萎基因组DNA为模板,对RAPD扩增反应条件进行优化,以期建立适合芜荽的RAPD的最优反应体系,结果表明:PCR扩增体系(总体积20μL)为:模板DNA1.0 ng·μL-1,dNTP 0.45 mmol·L-1,随机引物1.3μmol·L-1,Taq酶0.6 U,Mg2+2.0 mmol·L-1,退火温度39℃,循环次数40.

  11. Effect of preprocessing and compressed propane extraction on quality of cilantro (Coriandrum sativum L.).

    Science.gov (United States)

    Sekhon, Jasreen K; Maness, Niels O; Jones, Carol L

    2015-05-15

    Dehydration leads to quality defects in cilantro such as loss in structure, color, aroma and flavor. Solvent extraction with compressed propane may improve the dehydrated quality. In the present study, effect of drying temperature, particle size, and propane extraction on color, volatile composition, and fatty acid composition of cilantro was evaluated. Cilantro was dehydrated (40°C or 60°C), size reduced and separated into three particles sizes, and extracted with compressed propane at 21-27°C. Major volatile compounds found in dried cilantro were E-2-tetradecenal, dodecanal, E-2-dodecenal, and tetradecanal. Major fatty acids were linoleic acid and α-linolenic acid. Drying at 60°C compared to 40°C resulted in better preservation of color (decrease in browning index values) and volatile compounds. Propane extraction led to a positive change in color values and a decrease in volatile composition, oil content, and fatty acid composition.

  12. Study on Aromatic Components of Coriander Root%芫荽根部芳香成分研究

    Institute of Scientific and Technical Information of China (English)

    陆占国; 郭红转; 李伟

    2007-01-01

    为了使香菜根部得到有效利用,对香菜根部的香气成分进行了研究.采用水蒸气蒸馏方法对新鲜芫荽(香菜,Coriandrum sativum L.)根部的香气成分进行了提取,以0.304%收率获得了挥发油,用GC-MS方法对挥发油成分进行了分析,分析结果显示了共检测出66个成分,解析了占挥发油90.022%的39个成分,其中醛类49.422%,醇类18.868%,酯类6.481%,烃类5.249%.含量最多的是糠醛(23.050%),2-亚甲基环戊醇(14.389%)和2-十二烯醛(12.953%).由此,首次明确了黑龙江产香菜根部的香气成分.

  13. Biochemical Characterization of Coriander Cakes Obtained by Extrusion

    Directory of Open Access Journals (Sweden)

    Jazia Sriti

    2013-01-01

    Full Text Available This study was designed to examine the effect of operating conditions such as nozzle diameter on fatty acid, sterol, and tocol composition of coriander cakes. Eight fatty acids were identified, with petroselinic acid accounting for 75–77% of the total fatty acids, followed by linoleic, oleic, and palmitic acids, accounting for 12-13%, 5%, and 3%, respectively, of the total fatty acids. β-Sitosterol was the major sterol in all oils with 33–35% of total sterols. The next major sterols in all oils were stigmasterol (24% of total sterols and Δ7-stigmasterol (15% of total sterols. Coriander cake contained higher amounts of total tocotrienol where γ-tocotrienol was the main compound.

  14. Identification of a senescence-related protease in coriander leaves

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Senescence-related protease may play an important role in leaf senescence. By improved SDS-Gela- tin-PAGE assay, a 63 ku senescence-related protease (63 SRP) in coriander leaves was identified. Activity of 63 SRP was increased in parallel to the advance of coriander leaf senescence, and inhibited by treating the leaf with gibberellic acid, and enhanced by ethylene treatment. The 63 SRP was suggested to be a serine protease based on the fact that its activity was inhibited by the protease inhibitor PMSF. The optimal temperature for the activity of the 70 ku protease was 50℃. The maximal activity was observed at pH 6-9, some activity could be observed on the gel slices incubated at pH 5 or 11. The 63 SRP was partly purified by the way of ammonium sulfate precipitation and then gel slicing after gel electrophoresis.

  15. 75 FR 34687 - Notice of Decision to Issue Permits for the Importation of Fresh False Coriander From Panama Into...

    Science.gov (United States)

    2010-06-18

    ... Fresh False Coriander From Panama Into the Continental United States AGENCY: Animal and Plant Health... issuing permits for the importation into the continental United States of fresh false coriander from... noxious weeds via the importation of fresh false coriander from Panama. EFFECTIVE DATE: June 18, 2010....

  16. A genetic variant near olfactory receptor genes influences cilantro preference

    OpenAIRE

    Eriksson, Nicholas; Wu, Shirley; Do, Chuong B.; Kiefer, Amy K.; Joyce Y Tung; Mountain, Joanna L.; Hinds, David A.; Francke, Uta

    2012-01-01

    The leaves of the Coriandrum sativum plant, known as cilantro or coriander, are widely used in many cuisines around the world. However, far from being a benign culinary herb, cilantro can be polarizing---many people love it while others claim that it tastes or smells foul, often like soap or dirt. This soapy or pungent aroma is largely attributed to several aldehydes present in cilantro. Cilantro preference is suspected to have a genetic component, yet to date nothing is known about specific ...

  17. Supercritical CO2 extraction and analysis of aroma components of coriander leaf by GC/MS%超临界CO2萃取芫荽芳香成分及GC/MS解析

    Institute of Scientific and Technical Information of China (English)

    陆占国; 郭红转; 李伟; 杨书良

    2006-01-01

    采用超临界CO2萃取方法,对新鲜芫荽(Coriandrum sativum L.)茎叶进行萃取,得油率0.27%,用气相色谱/质谱联机(GC/MS)对精油成分进行了解析.共检测出60种成分,其中30种化合物被确定,占挥发油成分的82.772%.其中酯类35.046%,脂肪醛类22.792%,醇类15.641%,有机酸类5.357%,烃类3.936%.

  18. Physical properties of coriander seeds at different moisture content

    Science.gov (United States)

    Balasubramanian, S.; Singh, K. K.; Kumar, R.

    2012-10-01

    Physical properties of coriander seeds were determined at moisture content of 3.5-17.7%, d.b. The major axis and 1 000 seeds mass were found to decrease nonlinearly with increase in seed moisture. The medium and minor axes, geometric mean diameter, sphericity, unit volume, surface area and angle of repose increased linearly. Bulk density decreased linearly, however the true density increased non-linearly. The coefficient of static friction increased nonlinearly for different surfaces with increase in moisture level and its maximum was found for plywood surface. The rupture force and energy absorbed decreased linearly with increasing moisture content.

  19. Lack of Population Structure in Coriander Populations Based on SDS (Seed Storage Protein Page Analysis

    Directory of Open Access Journals (Sweden)

    Gülsüm Yaldiz

    2016-08-01

    Full Text Available Genetic variation is prerequisite for plant breeding. Nothing information existed in the literature for available diversity of Coriander accession in Turkey. Plant breeding activities are negligible in Turkey. So in order to start effective plant breeding program in Turkey, information on the available genetic diversity is viable. Therefore we planned to study the genetic variation and population structure of 29 Coriander accessions by seed storage protein (SDS. SDS analysis elaborated the lack of population structure and genetic bottleneck in the Coriander accessions in Turkey. Based on the results of this study, it was clear that sampling strategy was not appropriate and plant introduction should be made from different sources and diverse genotypes should be used as parents to initialize the effective Turkish Coriander breeding program.

  20. Effects of Coriander Essential Oil on the Performance, Blood Characteristics, Intestinal Microbiota and Histological of Broilers

    Directory of Open Access Journals (Sweden)

    S Ghazanfari

    2015-12-01

    Full Text Available ABSTRACT Present study was conducted to investigate the effects of the dietary supplementation of coriander oil on broiler performance, blood characteristics, microbiota, and small intestine morphology measurements. A number of one-day-old broiler chickens (Ross 308 were allocated to five treatments, with four replicates according to a completely randomized design (CRD. Birds were offered either a corn-soybean meal basal diet (control, or the basal diet supplemented with 600 mg/kg of a flavophospholipol antibiotic, 100, 200, or 300 mg/kg coriander essential oil. At 42 days of age, two birds per replicate were selected for blood collection, slaughtered, and its intestinal microbiota and morphology were investigated. The results indicated that weight gain, feed intake, and feed conversion ratio significantly improved by the dietary inclusion of the coriander oil and antibiotic compared with the control treatment (p0.05. Birds fed the coriander oil and antibiotic diets had lower populations of Escherichia coli than control group in cecum (p<0.05. The dietary treatments influenced the morphology of small intestinal villi. Birds fed antibiotic and coriander essential oil presented higher villus height and crypt depth compared with those in the control treatment (p<0.01. Coriander essential oil supplementation significantly decreased epithelial thickness and the number of goblet cell of the small intestinal compared with the control treatment (p<0.0001. In conclusion, coriander oil was shown to be an efficient growth promoter. The intestinal health improvement obtained with coriander oil was associated with improvements in broiler growth performance.

  1. Environ: E00269 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00269 Coriandrum sativum fruit Crude drug D-Linalool, Pinene [CPD:C06077], Bornyl acetate [CPD:C11338] Cori...andrum sativum [TAX:4047] Apiaceae (carrot family) Coriandrum sativum fruit Crude d...rugs [BR:br08305] Dicot plants: asterids Apiaceae (carrot family) E00269 Coriandrum sativum fruit ...

  2. Inoculación de Cilantro (Coriandrum sativum L. con Rizobacterias en Villa del Rosario, Norte de Santander / Inoculation of Cilantro (Coriandrum sativum L. with Rhizobacterias in Villa del Rosario, Norte de Santander

    Directory of Open Access Journals (Sweden)

    Katherine Carrillo Becerra

    2014-12-01

    Full Text Available Resumen. Las rizobacterias promotoras de crecimiento vegetalrepresentan una alternativa de biofertilización. En este estudiose evaluó el efecto de su inoculación en plantas de cilantro y lautilización de la práctica de quema de cascarilla de arroz en lapreparación del suelo para el establecimiento del cultivo. Seempleó un diseño experimental en parcelas divididas donde, lasparcelas principales correspondieron a la preparación del suelo conquema de cascarilla de arroz y sin quema con la inoculación previade Trichoderma sp. Las subparcelas eran los tratamientos con lainoculación simple y en co-inoculación de Azospirillum RzH132y Azotobacter RzH120 y los testigos absoluto y químico. Unavez se comprobaron los supuestos en los residuales del modelo,normalidad, homogeneidad de varianzas y aleatoriedad, se realizóel análisis de varianza y pruebas de comparación múltiple porel test de Tukey y un análisis de componentes principales comotécnica de reducción de dimensiones. Los resultados mostraronun efecto positivo en el crecimiento de las plantas inoculadas conlas rizobacterias en las dos parcelas con quema y sin quema decascarilla de arroz; sin embargo, en la variable rendimiento nose obtuvieron diferencias significativas (P≤0,05. Así mismo, seencontró que la población de bacterias rizosféricas en los mediosde cultivo NFb semisólido, Ashby y King B, se vio favorecida por lano quema de cascarilla de arroz en el suelo. Es importante resaltarque los resultados se obtuvieron con la disminución al 30% de lafertilización química, con lo cual se puede reducir el uso de estosproductos químicos. /  Abstract. Plant growth promoting rhizobacteria (PGPR representan alternative biofertilization form. In this study, was evaluatedPGPR inoculation in cilantro plants. Likewise, was evaluatedthe practice of burning rice husk in soil preparation for cropestablishment. An experimental design was used in a split plotwhere the main plots were soil preparation with burning ricehusk and without burning rice husk with prior inoculation ofTrichoderma sp. The subplots correspond to treatments withsimple inoculation and co-inoculation of Azotobacter RzH120and Azospirillum RzH132 and chemical and absolute control.Once the assumptions were tested in model residuals, normality,homogeneity of variance and randomness, an analysis of varianceand multiple comparison tests by Tukey's test and principalcomponent analysis technique was performed as size reduction.According to principal component analysis, the results showed apositive effect on the growth of plants inoculated with PGPR in thetwo plots with burning and without burning rice husk, however,the variable yield (P≤0.05. Also, it was found that the populationof bacteria in the rhizosphere culture media NFb semisolid, Ashbyand King B, was not favored by the burning of rice husks on the soil.Importantly, the results were obtained with the 30% decrease inthe chemical fertilizer, which can reduce the use of these chemicals.

  3. Design, formulation and evaluation of a mucoadhesive gel from Quercus brantii L. and coriandrum sativum L. as periodontal drug delivery

    Directory of Open Access Journals (Sweden)

    Abolfazl Aslani

    2013-01-01

    Conclusion: The ideal formulation for the treatment of periodontitis should exhibit high value of mucoadhesion, show controlled release of drug and be easily delivered into the periodontal pocket preferably using a syringe. Based on in vitro release and mucoadhesion studies, F 5 was selected as the best formulation.

  4. Precios y oferta para el culantro castilla (Coriandrum sativum L. entre los principales mercados mayoristas de Costa Rica y Panama

    Directory of Open Access Journals (Sweden)

    Alexis Villalobos Monge

    2012-05-01

    Full Text Available Este artículo muestra los resultados del análisis compa- rativo de precios y volúmenes ofertados del culantro de castilla en los principales mercados mayoristas de Costa Rica y Panamá. Como antecedente se tiene la ejecución de una gira de prospección realizada en el 2009 a Ciudad de Panamá por parte de los colaboradores del proyecto “Investigación de mercados agrícolas” de la Estación Ex- perimental Fabio Baudrit de la Universidad de Costa Rica. En esta gira se detectó que el culantro castilla presentaba interesantes oportunidades de mercado gracias al precio y a la calidad del producto ofertado, razón por la cual se definió, como línea de trabajo, profundizar en aspectos de esta índole para determinar las particularidades de los principales mercados mayoristas de cada país en el caso de este alimento. Se llevó a cabo una revisión de series de tiempo para datos de precio y oferta emitidos por las fuentes encargadas de cada mercado, con los que se reali- zaron comparaciones de estadísticos y análisis de tenden- cia por regresión lineal, los cuales permitieron dimensionar las diferencias entre ambos sitios para enmarcar el com- portamiento del culantro castilla como potencial agrone- gocio, relacionado con el consumo a nivel de cada país. Entre los principales hallazgos, se puede mencionar que en Costa Rica históricamente ha existido un mayor con- sumo de esta hortaliza en comparación con Panamá, sin embargo, la tendencia detectada en Panamá, en cuanto a precios y volúmenes ofertados, está creciendo a mayor velocidad en este último mercado. Las diferencias prome- dio de precios pagados en Panamá superan por más del 100% a los pagados en Costa Rica por la misma unidad de comercialización según series mensuales para un periodo de 4 años, razón por la que se afinó la observación y se analizaron las plazas de comercialización para un total de 246 plazas mayoristas, donde se encontró que en el 94% de las veces el precio fue mayor en el Mercado Central de Alimentos (MAC en Panamá que en el Centro Nacional de Abastecimiento y Distribución de Alimentos (Cenada en Costa Rica. Se descubrió que las plazas con mayores diferencias de precios a favor del MAC se efectuaron entre la tercera semana de mayo y las tres primeras semanas de junio, por lo que se asume que serían los momentos más oportu- nos para obtener el mejor precio posible para ingresar al mercado panameño con culantro castilla costarricense.

  5. 超声法提取香菜中绿原酸%Extraction of chlorogenic acid from Coriandrum sativum L.

    Institute of Scientific and Technical Information of China (English)

    邵红; 边才苗

    2005-01-01

    对香菜绿原酸的优选超声提取工艺条件进行了研究.采用正交实验法,考察溶剂浓度、超声时间、料液比、提取次数对提取率的影响,用分光光度标准曲线法测定含量.所考察的因素中,香莱绿原酸的提取影响程度为:溶剂浓度>提取次数>超声时间>料液比.超声提取的最佳条件A2B1C2D3.与常规的提取方法相比,具有提取时间短、简单、收率高、无需加热等优点.

  6. Characterization of French Coriander Oil as Source of Petroselinic Acid

    Directory of Open Access Journals (Sweden)

    Evelien Uitterhaegen

    2016-09-01

    Full Text Available Coriander vegetable oil was extracted from fruits of French origin in a 23% yield. The oil was of good quality, with a low amount of free fatty acids (1.8% and a concurrently high amount of triacylglycerols (98%. It is a rich source of petroselinic acid (C18:1n-12, an important renewable building block, making up 73% of all fatty acids, with also significant amounts of linoleic acid (14%, oleic acid (6%, and palmitic acid (3%. The oil was characterized by a high unsaponifiable fraction, comprising a substantial amount of phytosterols (6.70 g/kg. The main sterol markers were β-sitosterol (35% of total sterols, stigmasterol (24%, and Δ7-stigmastenol (18%. Squalene was detected at an amount of 0.2 g/kg. A considerable amount of tocols were identified (500 mg/kg and consisted mainly of tocotrienols, with γ-tocotrienol as the major compound. The phospholipid content was low at 0.3%, of which the main phospholipid classes were phosphatidic acid (33%, phosphatidylcholine (25%, phosphatidylinositol (17%, and phosphatidylethanolamine (17%. About 50% of all phospholipids were non-hydratable. The β-carotene content was low at 10 mg/kg, while a significant amount of chlorophyll was detected at about 11 mg/kg. An iron content of 1.4 mg/kg was determined through element analysis of the vegetable oil. The influence of fruit origin on the vegetable oil composition was shown to be very important, particularly in terms of the phospholipids, sterols, and tocols composition.

  7. Benefits of environmental conditions for growing coriander in Banat Region, Serbia.

    Science.gov (United States)

    Acimovic, Milica; Oljaca, Snezana; Jacimovic, Goran; Drazic, Slobodan; Tasic, Slavoljub

    2011-10-01

    As one of the oldest multi-purpose plants (spice, aromatic, honey and medicinal), coriander is widespread across Europe. Although in Serbia there are favorable conditions for its growth and development, it is grown on relatively small areas. During both investigated years it took more than 1200 degrees C for transfer from vegetative to generative phase of development and over 2000 degrees C for it to be ready for harvesting. Coriander is a photophilic plant, which requires around 1000 hours of light from sowing to ripening.. As for humidity, coriander grows well, if there are more than 200 mm of rainfall during growing season. In 2009 and 2010, the experiment carried out at the experimental field in Ostojićevo (Banat, Vojvodina province, Serbia) monitored the effect of parameters mentioned above on development of coriander plants, seed yield and essential oil content. The average yields of 1866 kg ha(-1) (2009) and 2470 kg ha(-1) (2010), and relatively high content of essential oil (1.06% in both years) indicate a great potential of this plant species in Serbia, which is, however, greatly dependent on environmental conditions during year.

  8. Evaluation of coriander spice as a functional food by using in vitro bioassays.

    Science.gov (United States)

    Zhang, Chuan-Rui; Dissanayake, Amila A; Kevseroğlu, Kudret; Nair, Muraleedharan G

    2015-01-15

    Coriander leaves and seeds are widely used as a condiment and spice. The use of roasted coriander seeds in food and beverage is very common. In this study, we investigated raw and roasted coriander seeds for their functional food quality using antioxidant, anti-inflammatory and human tumour cell proliferation inhibitory assays. The hexane and methanolic extracts of raw and roasted coriander seeds showed identical chromatographic and bioassay profiles. Chromatographic purification of the roasted seed extracts afforded tripetroselinin as the predominant component. Other isolates were petroselinic acid, 1,3-dipetroselinin, 2-C-methyl-d-erythritol, 2-C-methyl-d-erythritol 4-O-β-d-glucopyranoside and linalool. Hexane and methanolic extracts of both raw and roasted seeds and pure isolates from them showed comparable antioxidant and anti-inflammatory activities to the positive controls used in the assays, and inhibited the growth of human tumour cells AGS (gastric carcinoma), DU-145 and LNCaP (prostate carcinoma), HCT-116 (colon carcinoma), MCF-7 (breast carcinoma) and NCI-H460 (lung carcinoma) by 4-34%, respectively.

  9. Storage Quality Comparisons of Coriander and Water Spinach Between Red Light and Darkness at Room Temperature%光线照射下芫荽和蕹菜常温贮藏效果比较

    Institute of Scientific and Technical Information of China (English)

    顾振新; 陆兆新; 韩永斌; 郁志芳; 魏秀娟

    2003-01-01

    比较了芫荽(Coriandrum sativum L.)和蕹菜(Ipomoea aquatica Forsk)在20±1℃下光照处理的贮藏效果.在4d贮藏期内,用光合量子通量密度(PPED)为5μmol/m2·s的红光每天连续照射24h.结果表明,与黑暗条件下芫荽和蕹菜贮藏效果相比,光照有利于累积还原糖和减少叶绿素和VC损失,并且光照条件下蕹菜的贮藏效果好于芫荽;贮藏结束时,光照处理的芫荽和蕹菜中叶绿素含量分别比同期黑暗处理的高出76.82%和93.36%,还原糖含量分别高出32.69%和20.57%,VC保存率分别高出2.31和2.38倍;芫荽根部和蕹菜嫩茎切口吸收水分而使其保持鲜嫩状态,两者含水量变化与光照无关.

  10. Meristem cultures in garlic (Allium sativum) breeding

    OpenAIRE

    ŠVEHLOVÁ, Eva

    2017-01-01

    The diploma thesis deals with the use of meristem cultures in garlic (Allium sativum) breeding. The source material was used variety Tantalum of garlic. The using material, before the isolation of meristem, was tested for the occurrence of viral diseases by immunological tests ELISA (Enzyme-Linked Immuno-Sorbent Assay), also known as EIA (Enzyme Immunoassay). The method used to detect antibodies and antigens. The material was tested for viruses onion yellow dwarf (OYDV - Onion Yellow Dwarf Vi...

  11. Yield components and water use efficiency in coriander under irrigation and nitrogen fertilization

    Directory of Open Access Journals (Sweden)

    Karoline P. Angeli

    2016-05-01

    Full Text Available ABSTRACT Among the determining factors of yield, nitrogen (N fertilization and the correct supply of water play an important role in the quality and yield aspects of coriander. Therefore, the aim of this study was to evaluate the yield components and the water use efficiency (WUE in coriander subjected to different depths and N doses. A completely randomized design in split plot was used. Water depths were applied in the plots at the rates of 25, 50, 75, 100 and 125% of the Required Real Irrigation (RRI. N doses were distributed within the subplots at the following proportions: 50, 100 and 150% of the recommendation for the crop (70 kg ha-1. The N dose of 94 kg ha-1 and irrigation depth of 115% of RRI promoted the greatest yield (29.0 t ha-1 and number of bunches (29 bunches m-2. The highest number of leaves per plant was obtained with the N dose of 103.2 kg ha-1 and irrigation depth of 68% of the RRI. The maximum plant height (43 cm was obtained with N dose of 105 kg ha-1 and irrigation depth of 121% of RRI. The highest WUE in coriander (71 kg m-3 occurred at the irrigation depth of 26% of RRI and N dose of 105 kg ha-1.

  12. Toxicological evaluation of the aqueous extract of Allium sativum ...

    African Journals Online (AJOL)

    Toxicological evaluation of the aqueous extract of Allium sativum bulbs on laboratory mice and rats. ... PROMOTING ACCESS TO AFRICAN RESEARCH ... The possible toxicological risks of Allium sativum aqueous extract upon consumption ... FAQ's · News · AJOL jobs · More about AJOL · AJOL's Partners · Contact AJOL ...

  13. Evaluation of Biomass Yield and Water Treatment in Two Aquaponic Systems Using the Dynamic Root Floating Technique (DRF)

    OpenAIRE

    Laura Silva; Eucario Gasca-Leyva; Edgardo Escalante; Kevin M Fitzsimmons; David Valdés Lozano

    2015-01-01

    The experiment evaluates the food production and water treatment of TAN, NO2−–N, NO3−–N, and PO43− in two aquaponics systems using the dynamic root floating technique (DRF). A separate recirculation aquaculture system (RAS) was used as a control. The fish cultured was Nile tilapia (Oreochromis niloticus). The hydroponic culture in one treatment (PAK) was pak choy (Brassica chinensis,) and in the other (COR) coriander (Coriandrum sativum). Initial and final weights were determined for the fis...

  14. Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander.

    Science.gov (United States)

    Gómez-Aldapa, Carlos A; Segovia-Cruz, Jesús A; Cerna-Cortes, Jorge F; Rangel-Vargas, Esmeralda; Salas-Rangel, Laura P; Gutiérrez-Alcántara, Eduardo J; Castro-Rosas, Javier

    2016-10-01

    The prevalence and behavior of multidrug-resistant diarrheagenic Escherichia coli pathotypes on coriander was determined. One hundred coriander samples were collected from markets. Generic E. coli were determined using the most probable number procedure. Diarrheagenic E. coli pathotypes (DEPs) were identified using two multiplex polymerase chain reaction procedures. Susceptibility to sixteen antibiotics was tested for the isolated DEPs strains by standard test. The behavior of multidrug-resistant DEPs isolated from coriander was determined on coriander leaves and chopped coriander at 25°± 2 °C and 3°± 2 °C. Generic E. coli and DEPs were identified, respectively, in 43 and 7% of samples. Nine DEPs strains were isolated from positive coriander samples. The identified DEPs included Shiga toxin-producing E. coli (STEC, 4%) enterotoxigenic E. coli (ETEC, 2%) and enteropathogenic E. coli (EPEC, 1%). All isolated DEPs strains exhibited multi-resistance to antibiotics. On inoculated coriander leaves stored at 25°± 2 °C or 3°± 2 °C, no growth was observed for multidrug-resistant DEPs strains. However, multidrug-resistant DEPs strains grew in chopped coriander: after 24 h at 25° ± 2 °C, DEPs strains had grown to approximately 3 log CFU/g. However, at 3°± 2 °C the bacterial growth was inhibited. To the best of our knowledge, this is the first report of the presence and behavior of multidrug-resistant STEC, ETEC and EPEC on coriander and chopped coriander.

  15. [The effects of grape seed and coriander oil on biochemical parameters of oral fluid in patients with periodontitis].

    Science.gov (United States)

    Nikolaishvili, M; Gogua, M; Franchuki, Q; Tufinashvili, T; Zurabashvili, D

    2014-10-01

    Biochemical changes that are taking place in patients with periodontitis 1 and 2 the quality of the individuals place in a laqtatdegidrogenazis, alkaline phosphatase activity increase and reduced activity amilazais. Therefore we can conclude that, there is an increase in superoxide substances, which causes an increase in the oral cavity patobakteriebis and quality of periodontitis. The grape seed and coriander oil is of vegetable origin and antioxidant drugs. Their action causes a statistically significant increase in the amilazis, alkaline phosphatase and laqtatdegidrogenazis reduction, while the latter leads pH - rate of return to oral fluid. It should be noted that the positive effect of coriander oil, but less effective.

  16. 75 FR 6345 - Notice of Availability of a Pest Risk Analysis for the Importation of Fresh False Coriander From...

    Science.gov (United States)

    2010-02-09

    ... Animal and Plant Health Inspection Service Notice of Availability of a Pest Risk Analysis for the... prepared a pest risk analysis that evaluates the risks associated with the importation into the continental... introducing or disseminating plant pests or noxious weeds via the importation of fresh false coriander...

  17. Preharvest treatments with malic, oxalic, and acetylsalicylic acids affect the phenolic composition and antioxidant capacity of coriander, dill and parsley.

    Science.gov (United States)

    El-Zaeddi, Hussein; Calín-Sánchez, Ángel; Nowicka, Paulina; Martínez-Tomé, Juan; Noguera-Artiaga, Luis; Burló, Francisco; Wojdyło, Aneta; Carbonell-Barrachina, Ángel A

    2017-07-01

    The effects of a preharvest treatment with malic (MA), oxalic (OA), or acetylsalicylic (ASA) acid at three concentrations (1, 2 and 3mM) on the bioactivity and antioxidant capacity of coriander, dill, and parsley were investigated. The antioxidant capacity of the herbs extracts was assayed by spectrophotometric methods by using three different analytical methods: ORAC, FRAP, and ABTS; the effects of treatments were very positive in coriander, produced intermediate results in dill, and no effects were found in parsley plants. Polyphenol compounds were identified by LC-MS-QTof and quantified by UPLC-PDA-FL. Thirty phenolic compounds were identified in these three herbs. The major compounds were (i) coriander: dimethoxycinnamoyl hexoside and quercetin-3-O-rutinoside, (ii) dill: neochlorogenic acid and quercetin glucuronide, and (iii) parsley: apigenin-7-apiosylglucoside (apiin) and isorhamnetin-3-O-hexoside. The application of these three organic acids favored the accumulation of phenolic compounds in coriander plants, but had no significant positive effects on dill and parsley. The treatments leading to the best results in all three plants were the application of MA or OA at 1mM.

  18. Molecular genetic diversity study of Lepidium sativum population ...

    African Journals Online (AJOL)

    Vostro 2520

    (NJ) and PCO analysis showed very weak grouping among individuals collected from the same regions. .... compare the population and generate phenogram using NTSYS- pc ..... with 85 L. sativum accessions showed four distinct clusters.

  19. Water use efficiency of coriander produced in a low-cost hydroponic system

    Directory of Open Access Journals (Sweden)

    José A. Santos Júnior

    2015-12-01

    Full Text Available ABSTRACT The increase of water use efficiency in crop production is a clear need in areas with restricted access to this resource and, in these cases, the adoption of forms of cultivation contextualized to local conditions are essential. Thus, the implications of the variation in the amount of seeds per cell (0.5, 1.0, 1.5 and 2.0 g and spacing between cells (7.0, 10.0 and 15.0 cm on variables related to consumption and water use efficiency for the production of coriander (cv. Tabocas in a low-cost hydroponic system, an alternative for semiarid regions, were evaluated. A completely randomized experimental design, analysed in 4 x 3 factorial scheme with three replicates, was adopted, and the data were subjected to analysis of variance at 0.05 probability level. It was found that the reduction in the spacing between cells has a better cost-benefit ratio with respect to water consumption, biomass produced and cost of seeds. Therefore, it is recommended the adoption of a spacing of 7.0 cm between cells and the use of 1.0 g seeds per cell; this configuration promoted efficiency of 81.59 g L-1 in shoot green mass production and total mass of 62.4 g coriander bunches.

  20. Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco

    Energy Technology Data Exchange (ETDEWEB)

    Cahoon, E.B.; Shanklin, J.; Ohlrogge, J.B. (Michigan State Univ., East Lansing (United States))

    1992-12-01

    Little is known about the metabolic origin of petroselinic acid (18:1[Delta][sup 6cis]), the principal fatty acid of the seed oil of most Umbelliferae, Araliaceae, and Garryaceae species. To examine the possibility that petroselinic acid is the product of an acyl-acyl carrier protein (ACP) desaturase, Western blots of coriander and other Umbelliferae seed extracts were probed with antibodies against the [Delta][sup 9]-stearoyl-ACP desaturase of avocado. In these extracts, proteins of 39 and 36 kDa were detected. Of these, only the 36-kDa peptide was specific to tissues which synthesize petroselinic acid. A cDNA encoding the 36-kDa peptide was isolated from a coriander endosperm cDNA library, placed under control of the cauliflower mosaic virus 35S promoter, and introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. Expression of this cDNA in transgenic tobacco callus was accompanied by the accumulation of petroselinic acid and [Delta][sup 4]-hexadecenoic acid, both of which were absent from control callus. These results demonstrate the involvement of a 36-kDa putative acyl-ACP desaturase in the biosynthetic pathway of petroselinic acid and the ability to produce fatty acids of unusual structure in transgenic plants by the expression of the gene for this desaturase. 27 refs., 5 figs.

  1. Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco.

    Science.gov (United States)

    Cahoon, E B; Shanklin, J; Ohlrogge, J B

    1992-12-01

    Little is known about the metabolic origin of petroselinic acid (18:1 delta 6cis), the principal fatty acid of the seed oil of most Umbelliferae, Araliaceae, and Garryaceae species. To examine the possibility that petroselinic acid is the product of an acyl-acyl carrier protein (ACP) desaturase, Western blots of coriander and other Umbelliferae seed extracts were probed with antibodies against the delta 9-stearoyl-ACP desaturase of avocado. In these extracts, proteins of 39 and 36 kDa were detected. Of these, only the 36-kDa peptide was specific to tissues which synthesize petroselinic acid. A cDNA encoding the 36-kDa peptide was isolated from a coriander endosperm cDNA library, placed under control of the cauliflower mosaic virus 35S promoter, and introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. Expression of this cDNA in transgenic tobacco callus was accompanied by the accumulation of petroselinic acid and delta 4-hexadecenoic acid, both of which were absent from control callus. These results demonstrate the involvement of a 36-kDa putative acyl-ACP desaturase in the biosynthetic pathway of petroselinic acid and the ability to produce fatty acids of unusual structure in transgenic plants by the expression of the gene for this desaturase.

  2. FORMULATION AND EVALUATION OF DENTAL GEL CONTAINING ESSENTIAL OIL OF CORIANDER AGAINST ORAL PATHOGENS

    Directory of Open Access Journals (Sweden)

    Pawar Vinita A

    2013-10-01

    Full Text Available The objective of this study was to investigate the antimicrobial potency of essential oil of Coriander against clinical samples isolated from the oral cavity of various patients and to formulate a suitable topical preparation containing Coriander oil. A total of 41 swabs were obtained from patients (age between 20 - 50 years which were diagnosed with oral infections like periodontal abscess, periapical abscess, chronic periodontities, periapical granuloma, dental caries, root caries and plaque samples. These samples were inoculated on Blood agar, Mac-conkeys agar and MRS Lactobacilli MRS agar, Mutans Sanguis agar, KF Streptococcal agar at 37°C for 24 – 48 h aerobically except for KF Streptococcal agar and Mutans Sanguis agar, which was incubated anaerobically at 37°C for 24 – 48 h. The isolates were identified by standard microbiological procedures. The samples had positive cultures of Streptococcus faecalis, Streptococcus sanguis, Streptococcus oralis, Streptococcus mutans, Streptococcus salivarius, Lactobacilli, CONS. The susceptibility of these isolates was determined using agar disc diffusion method for zone of inhibition. The evaluation of antimicrobial potency was studied prior to gel formulation to compare the changes in activity after incorporation in polymer gel. The topical formulations were developed using different concentrations of polymers and evaluated for various physicochemical parameters like pH, color, clarity, viscosity, consistency, homogeneity, spread ability. The activity of oil was not much affected by incorporation in gel. The gel showed promising antimicrobial activity against the strains used for the study.

  3. Glucolipids of Zea mays and Pisum sativum

    Energy Technology Data Exchange (ETDEWEB)

    Morohashi, Y.; Bandurski, R.S.

    1976-06-01

    The glucolipids formed upon feeding (U--/sup 14/C)glucose to embryos of Zea mays were partially characterized with respect to: (a) metabolic turnover, (b) acid lability, (c) phosphorus content, (d) chromatographic properties, and (e) hydrolysis products. The chloroform--methanol-soluble-assimilated radioactivity was examined specifically for occurrence of a glycosylated prenol phosphate. With the extraction conditions used, no evidence was found for formation of a glucosylated prenol phosphate. Several, as yet unidentified, acid-labile glucolipids undergoing metabolic turnover were observed. Four diglycerides were characterized as hydrolysis products of a fraction that contained /sup 14/C-glucose and phosphorus, and was subject to metabolic turnover. Examination of the 1-butanol-soluble glucolipids from pea (Pisum sativum) seedlings also demonstrated anionic glucolipids, evidencing metabolic turnover but none with the properties of glucosylated prenol phosphate.

  4. Comercialización de semillas importadas de cilantro coriandrum sativum l. y zapallo cucurbita moschata durante el periodo enero 1990-agosto 1994

    OpenAIRE

    Lema Nuñez, Rosario M.

    2010-01-01

    Las cantidades importadas de semillas de cilantro (20.393 kg)  y de zapallo (1.518 Kg.) representaron el 3.2% del volumen total para hortalizas (648.000 kilos) y el 0.79% del valor total de dichas importaciones ($8.900 millones). Por la alta concentración del mercado colombiano en 4 ó 5 empresas -productoras y distribuidoras-, constituyen oligopolios, los cuales impiden la entrada de nuevas empresas diferenciando las semillas en forma real (calidad) y no real (marca, color del empaque). Las m...

  5. Determination of Linalool in the Volatile Oil of Coriandrum sativum L.%芫荽子挥发油中芳樟醇含量测定

    Institute of Scientific and Technical Information of China (English)

    张建民; 宋育航; 王甲林

    2004-01-01

    目的测定芫荽子挥发油中芳樟醇含量.方法采用内标法,以乙酸龙脑酯为内标物,测定了陕西、北京、安徽、河南、广东等地产芜荽子挥发油中芳樟醇含量.结果不同产地的芫荽子挥发油含量以及挥发油中芳樟醇含量均有一定差异.结论方法简单,重现性好,可作为芜荽子挥发油中芳樟醇含量测定方法.

  6. Rendimento do coentro (Coriandrum sativum L adubado com esterco bovino em diferentes doses e tempos de incorporação no solo

    Directory of Open Access Journals (Sweden)

    P.C.F LINHARES

    2015-09-01

    Full Text Available RESUMO O uso de esterco bovino é de suma importância para os agricultores familiares que produzem coentro na região de Mossoró-RN, pois esse insumo é amplamente disponível e utilizado pelos agricultores. Objetivando-se avaliar o Rendimento do coentro fertilizado com esterco bovino em diferentes doses e tempos de incorporação ao solo foi conduzido um experimento no período de setembro a novembro de 2011, na Fazenda Experimental Rafael Fernandes, da Universidade Federal Rural do Semi-Árido - UFERSA, Brasil. Os tratamentos consistiram da combinação de quatro doses de esterco bovino incorporadas ao solo: 15,0; 30,0; 45,0 e 60,0 t ha-1 em base seca, com quatro tempos de incorporação: 28; 49; 64 e 80 dias antes da semeadura do coentro - DAS, mais um tratamento controle (ausência de adubação. O delineamento experimental utilizado foi em blocos completos casualizados com os tratamentos arranjados em esquema fatorial 4 x 4 + 1 com 3 repetições. A cultivar de coentro utilizado foi a Verdão e as variáveis determinadas foram altura e número de hastes por planta e rendimento de coentro. O coentro respondeu à aplicação de esterco bovino, produzindo rendimentos máximos de 6453 e 6349 kg ha-1 de massa verde, com a dose de 60 t ha-1 e aos quarenta e seis dias de incorporação antes da semeadura, respectivamente.

  7. 不同盐浓度对香菜光合色素含量的影响%Effects of Salinity on Photosynthetic Pigment Production of Coriandrum sativum

    Institute of Scientific and Technical Information of China (English)

    曲英敏; 孙立志; 张学涛; 杨洲红; 李景梅

    2014-01-01

    [目的]明确不同盐浓度对香菜光合色素的生理效应.[方法]以自行培养的香菜作为试验材料,采用紫外分光光度法研究了不同浓度的盐胁迫对香菜叶绿素a、叶绿素b、叶绿素总含量、类胡萝卜素及色素含量的影响.[结果]试验表明,Na+低浓度(50 mmol/L)胁迫时,香菜中叶绿素等有机物有所积累,100 mmol/L盐浓度处理的香菜中叶绿素含量最高,营养物质积累最多,当盐浓度超过200mmol/L后香菜的生长受到明显的抑制作用.[结论]研究可为香菜生产的盐浓度调节提供参考依据.

  8. 香菜挥发油GC/MS测定%Determination of Volatile Oil of Coriandrum Sativum L.by GC/MS

    Institute of Scientific and Technical Information of China (English)

    孙小媛; 马玉芳; 李铁纯; 辛广

    2002-01-01

    对香菜茎叶及根经水蒸汽蒸馏--萃取得到的挥发油进行气相色谱/质谱(GC/MS)分析.其中香菜茎叶中主要成分有:壬烷、癸烷、癸醛、十一醛、环癸烷、十二醛、2-十一烯醛、2-癸烯-1-醇、十三醛、十四醛等,香菜根挥发油中主要成分有:壬烷、辛醛、癸醛、环癸烷、十-醛、十二醛、十四醛等.

  9. 香菜乙醇提取液的体外抗氧化活性%STUDY ON ANTIOXIDANT ACTIVITIES OF ETHANOL EXTRACTS FROM CORIANDRUM SATIVUM IN VITRO

    Institute of Scientific and Technical Information of China (English)

    陈志红; 徐美奕

    2009-01-01

    采用Fenton反应体系产生羟自由基和邻苯三酚自氧化产生超氧阴离子自由基,研究香菜乙醇提取液对两者的清除作用.并以芦丁为时照品,测定其总黄酮含量.结果表明:香菜中总黄酮含量为8.493 mg/g.香菜乙醇提取液对羟自由基和超氧阴离子自由基均有清除作用,半数清除量(IC50)分剐为122.331 mg/L和71.535 mg/L,提取物浓度的添加量在试验范围内与其抗氧化活性呈正相关.

  10. STUDY ON ULTRASONIC EXTRACTION TECHNICS OF FLAVONOIDS IN CORIANDRUM SATIVUM%香菜总黄酮的超声提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    邵红; 边才苗; 杨蓓芬; 王锦文

    2005-01-01

    确定香菜总黄酮的优选超声提取工艺条件.采用正交试验法,考察溶剂浓度、超声时间、料液比、提取次数对提取率的影响,用分光光度标准曲线法测定含量.所考察的因素中,香菜总黄酮的提取影响程度为:溶剂浓度>提取次数>超声时间>料液比.超声提取的最佳条件为95%乙醇,超声30min,料液比1:20,提取4次.与常规的提取方法相比,具有提取时间短、简单、收率高无需加热等优点.

  11. 香菜微量元素的测定分析%Determination and Analysis of Trace Elements in Coriandrum sativum

    Institute of Scientific and Technical Information of China (English)

    陈志红; 揭新明

    2008-01-01

    目的:应用电感耦合等离子体发射光谱法(ICP-AES)测定了芜萎茎与叶中14种无机元素的含量.方法:用干法灰化样品,用外标法测定.结果:芜萎中钙、馒、铁、锌、锰、铜含量丰富,有害元素铅含量也比较高.茎与叶微量元素含量有盖异.结论:芜姜含有较丰富的人体所需的微量元素.常少量食用.对身体健康有益.

  12. 香菜提取液的抗氧化活性初步研究%Antioxidant Activity of Coriandrum sativum Extract

    Institute of Scientific and Technical Information of China (English)

    卢冬梅; 蔺红萍; 文静; 周鲜娇; 肖生鸿

    2015-01-01

    采用煮制法提取香菜中的有效成分,研究其体外抗氧化活性.运用硝酸铝显色法,检测总黄酮含量,并测定香菜提取液的总抗氧化能力以及其对羟自由基的清除能力.结果表明,芦丁在0.01~0.1 mg/mL (R2=0.999 1)质量浓度范围内,吸光度与浓度之间呈良好线性关系,香菜提取液的黄酮含量为1.73 mg/g鲜重;香菜具有显著的抗氧化活性,且呈剂量依赖性,总抗氧化能力为44.19 mg VC/g鲜重,其清除羟自由基的能力IC50=67.11 mg/mL.由此说明了香菜具有丰富的黄酮类化合物,且具有良好的体外抗氧化活性.

  13. Aspectos culturais e prospecção de compostos com actividade biológica do coentro (Coriandrum sativum L.)

    OpenAIRE

    Delgado, Fernanda Maria Grácio

    1993-01-01

    Quatro anos de cultura do coentro em Castelo Branco permitiram concluir que a data de sementeira que melhor resultou em termos de precocidade e produção de raízes, folhas e frutos, foi a registada em finais de Fevereiro, obtendo-se produções médias por planta de 4g de peso seco de raízes, 9g de peso seco de folhas e 9g de peso seco de frutos. Simultaneamente, estudou-se a presença nesta espécie de certas substâncias com acção pesticida. Com efeito, actualmente, a utilização crescente do pe...

  14. 无土气雾式栽培对香菜品质的影响%Effect of Soilless Aeroponics on Coriandrum sativum Quality

    Institute of Scientific and Technical Information of China (English)

    丁一; 张路; 于超; 盛祥; 王华森

    2013-01-01

    以“四季香菜”为试材,以基质栽培香菜为对照,采用无土气雾式栽培方法,研究了气雾栽培对香菜品质的影响.结果表明:气雾栽培条件下的香菜维生素C含量为70.7 mg/100g,较基质栽培的香菜维生素C含量(37.8mg/100g)有显著提高;气雾培香菜,矿质元素硒的含量为0.021 mg/g,较基质培香菜硒含量(0.010 mg/g)有显著提高;气雾培香菜氨基酸含量较基质栽培的香菜略有提高,但差异不显著.

  15. Aspectos relacionados à qualidade de sementes de coentro Aspects related to coriander seed quality

    Directory of Open Access Journals (Sweden)

    Roseane S. Pereira

    2005-07-01

    Full Text Available O coentro é uma hortaliça amplamente consumida no Brasil como condimento, de grande importância socio-econômica. Problemas relacionados ao baixo vigor de sementes e ao estabelecimento da cultura são uma constante nesta espécie. No presente trabalho 11 lotes comerciais de sementes de coentro das cultivares Verdão, Americano Gigante, Tapacurá, Tabocas, Palmeira e Asteca foram analisados quanto a pureza, germinação, vigor e qualidade sanitária. Em outro estudo, avaliaram-se os efeitos das temperaturas de 15; 20; 25; 30 e 35ºC sobre a germinação das sementes das mesmas cultivares do experimento anterior e mais a cultivar Verdão HV. Houve variação relativamente pequena da germinação entre os lotes e com valores superiores ao padrão nacional para comercialização de sementes. Apenas o lote 1 apresentou pureza abaixo do referido padrão. A qualidade das sementes de coentro comercializadas pelas empresas de sementes no país é, geralmente, satisfatória. Quanto à sanidade, foi detectada a presença de alguns fungos de importância para a cultura do coentro, como Alternaria alternata e Alternaria radicina, com índices de associação elevados. Em um lote, foi detectada a presença de Alternaria dauci. Verificou-se efeito significativo da temperatura, de cultivar e da interação entre a cultivar e temperatura na germinação das sementes. Temperaturas entre 15 e 25ºC proporcionaram maior germinação, enquanto as temperaturas mais elevadas foram prejudiciais. As cultivares 'Verdão HV' e 'Palmeira' germinaram satisfatoriamente a 30ºC. Praticamente não ocorreu germinação a 35ºC para todas as cultivares avaliadas.Coriander is a vegetable crop with large consumption in Brazil, of high social and economic importance. Problems related to low seed vigor and cultivation are reported in this crop. In the present study, eleven seed lots of coriander cultivars 'Verdão', 'Americano Gigante', 'Tapacurá', 'Tabocas', 'Palmeira' and

  16. THE YIELD AND COMPOSITION OF ESSENTIAL OIL FROM SPLIT CORIANDER FRUITS

    Directory of Open Access Journals (Sweden)

    Mustafayev S. K.

    2016-04-01

    Full Text Available The article studies dynamics of losses and the change of composition of essential oil from split coriander fruits. It is found, that in the fractions of split fruits, extracted from long-stored commercial lots of raw materials, mass fraction of essential oil is two or three times lower than in whole fruits. In the composition of essential oil from split fruits the content of valuable components - linalool, geraniol, geranyl acetate is slightly higher, and the content of undesirable hydrocarbons and camphor is lower. It is shown that from freshly split fruits, which were stored in a thin layer in the open air, the oil was intensively lost in the first three days, the losses reached 86 %. At the same time, the composition of the oil changed: the content of most volatile components - hydrocarbons decreased several times and the content of high-boiling linalool, geraniol, geranyl acetate significantly increased, which increased the quality of the oil smell. The change of composition is determined not only by the ratio of components volatility. The content of relatively high boiling camphor almost half decreased. This could be associated with less ability of fruit tissue to sorb and the ability of camphor to be easily sublimated. The content of volatile n-cymene over time increased with a simultaneous decrease in the content of γ– terpinene, which confirmed predominantly chemical way of n-cymene accumulation in coriander essential oil in conditions conducive to oxidation. It is recommended to separate the split fruits as soon as the raw materials come to the plant and to process immediately. Essential oil from split fruits can be used to adjust the composition of individual lots of oil in order to improve their quality, and to provide extraction of valuable components – linalool and geraniol by vacuum rectification method

  17. Microstructural, spectroscopic, and antibacterial properties of silver-based hybrid nanostructures biosynthesized using extracts of coriander leaves and seeds

    Science.gov (United States)

    Luna, Carlos; Barriga-Castro, Enrique Díaz; Gómez-Treviño, Alberto; Núñez, Nuria O; Mendoza-Reséndez, Raquel

    2016-01-01

    Coriander leaves and seeds have been highly appreciated since ancient times, not only due to their pleasant flavors but also due to their inhibitory activity on food degradation and their beneficial properties for health, both ascribed to their strong antioxidant activity. Recently, it has been shown that coriander leaf extracts can mediate the synthesis of metallic nanoparticles through oxidation/reduction reactions. In the present study, extracts of coriander leaves and seeds have been used as reaction media for the wet chemical synthesis of ultrafine silver nanoparticles and nanoparticle clusters, with urchin- and tree-like shapes, coated by biomolecules (mainly, proteins and polyphenols). In this greener route of nanostructure preparation, the active biocompounds of coriander simultaneously play the roles of reducing and stabilizing agents. The morphological and microstructural studies of the resulting biosynthesized silver nanostructures revealed that the nanostructures prepared with a small concentration of the precursor Ag salt (AgNO3 =5 mM) exhibit an ultrafine size and a narrow size distribution, whereas particles synthesized with high concentrations of the precursor Ag salt (AgNO3 =0.5 M) are polydisperse and formation of supramolecular structures occurs. Fourier transform infrared and Raman spectroscopy studies indicated that the bioreduction of the Ag− ions takes place through their interactions with free amines, carboxylate ions, and hydroxyl groups. As a consequence of such interactions, residues of proteins and polyphenols cap the biosynthesized Ag nanoparticles providing them a hybrid core/shell structure. In addition, these biosynthesized Ag nanomaterials exhibited size-dependent plasmon extinction bands and enhanced bactericidal activities against both Gram-positive and Gram-negative bacteria, displaying minimal inhibitory Ag concentrations lower than typical values reported in the literature for Ag nanoparticles, probably due to the synergy of

  18. THE STUDY OF THE FEATURES OF DEPRIVED OF ETHER CORIANDER FRUITS AS RAW MATERIALS FOR FATTY OIL PRODUCTION BY MEANS OF PRESSING

    Directory of Open Access Journals (Sweden)

    Mustafayev S. K.

    2015-11-01

    Full Text Available The article shows that for the essential oil industry of the Russian Federation to solve the problems of import substitution it is urgent to develop effective technology to extract fatty oil from the crushed and deprived of ether by water vapor coriander fruits. The existing technologies for obtaining fatty oils from deprived of ether coriander were considered. It is shown, that at small-scale enterprises it is most advisable to remove fatty oil by screw pressing. A brief analysis of main factors affecting the completeness of oil extraction in screw presses was carried out. The conclusion about the necessity of preparation of deprived of ether coriander for extraction with the purpose of increasing the yield of fatty oil was made. It is shown, that one of the stages of such preparation is the removal of part of the shell from deprived of ether coriander, which also increases the nutritional value of the oilcake. Fractional composition of deprived of ether coriander after extraction of essential oil by water vapour and drying was investigated by sieve analysis method. On the basis of obtained data, prior to separating the shell by pneumatic separation, it is recommended to separate deprived of ether coriander into three fractions by size – coarse (riddling from the sieve with the diameter of 1.8 mm, medium (riddling from the sieve with the diameter of 1 mm and fine (undersize from a sieve with a diameter of 1 mm. Component composition of the coarse fraction of deprived of ether coriander was studied. It was found, that the content of the shell in this fraction is 36%, which indicates the possibility of its effective separation by pneumatic separation

  19. Some Comparative Histomorphometrical Aspects Regarding Detoxifying Capacity of Garlic, Coriander and Chlorella, in Chronic Cd Contamination on Carassius gibelio Species

    Directory of Open Access Journals (Sweden)

    Mărioara Nicula

    2014-11-01

    Full Text Available The purpose of this study was to histomorphometrically compare the aspect of gill, intestinal and ovarian epithelium of Prussian carp specimens, simultaneously subjected to chronic intoxication with Cd and, to chelating and antioxidant effect of chlorella, coriander and garlic on this toxicant respectively. 150 Prussian carps, 10-12 g of weight were divided according to the following treatments for 21 days: C (without treatment, E1 (10 ppm Cd into water, E2 (10 ppm Cd into water+2% lyophilized garlic in feed, E3 (10 ppm Cd into water+2% lyophilized coriander in feed, E4 (10 ppm Cd into water+2% lyophilized chlorella in feed. Fragments of gill, small intestine and ovary were removed and analyzed by light microscopy and a specific QuickPHOTO Micro 2.2 software has been used for the histomorphometric study. Mentioned epithelium suffered evident histomorphologic and histomorphometric changes under the action of Cd and Cd plus chlorella, Cd plus coriander and Cd plus garlic respectively. Statistical processing data related to the gill lamellae length, intestinal villi height and chorion thickness of ovaries follicles revealed the existence of different degrees of significance between experimental groups compared.

  20. Antibacterial activity of natural spices on multiple drug resistant Escherichia coli isolated from drinking water, Bangladesh.

    Science.gov (United States)

    Rahman, Shahedur; Parvez, Anowar Khasru; Islam, Rezuanul; Khan, Mahboob Hossain

    2011-03-15

    Spices traditionally have been used as coloring agents, flavoring agents, preservatives, food additives and medicine in Bangladesh. The present work aimed to find out the antimicrobial activity of natural spices on multi-drug resistant Escherichia coli isolates. Anti-bacterial potentials of six crude plant extracts (Allium sativum, Zingiber officinale, Allium cepa, Coriandrum sativum, Piper nigrum and Citrus aurantifolia) were tested against five Escherichia coli isolated from potable water sources at kushtia, Bangladesh. All the bacterial isolates were susceptible to undiluted lime-juice. None of them were found to be susceptible against the aqueous extracts of garlic, onion, coriander, pepper and ginger alone. However, all the isolates were susceptible when subjected to 1:1:1 aqueous extract of lime, garlic and ginger. The highest inhibition zone was observed with lime (11 mm). Natural spices might have anti-bacterial activity against enteric pathogens and could be used for prevention of diarrheal diseases. Further evaluation is necessary.

  1. Plant foods in the management of diabetes mellitus: spices as beneficial antidiabetic food adjuncts.

    Science.gov (United States)

    Srinivasan, K

    2005-09-01

    Diet has been recognized as a corner stone in the management of diabetes mellitus. Spices are the common dietary adjuncts that contribute to the taste and flavour of foods. Besides, spices are also known to exert several beneficial physiological effects including the antidiabetic influence. This review considers all the available information from animal experimentation as well as clinical trials where spices, their extracts or their active principles were examined for treatment of diabetes. Among the spices, fenugreek seeds (Trigonella foenumgraecum), garlic (Allium sativum), onion (Allium cepa), and turmeric (Curcuma longa) have been experimentally documented to possess antidiabetic potential. In a limited number of studies, cumin seeds (Cuminum cyminum), ginger (Zingiber officinale), mustard (Brassica nigra), curry leaves (Murraya koenigii) and coriander (Coriandrum sativum) have been reported to be hypoglycaemic.

  2. Microstructural, spectroscopic, and antibacterial properties of silver-based hybrid nanostructures biosynthesized using extracts of coriander leaves and seeds

    Directory of Open Access Journals (Sweden)

    Luna C

    2016-09-01

    Full Text Available Carlos Luna,1 Enrique Díaz Barriga-Castro,2 Alberto Gómez-Treviño,3 Nuria O Núñez,4 Raquel Mendoza-Reséndez1 1Research Center of Mathematics and Physics, Faculty of Mathematics and Physics, Autonomous University of Nuevo León, Nuevo León, Mexico; 2Central Laboratory of Analytical Instrumentation, Research Center for Applied Chemistry, Coahuila, Mexico; 3Laboratory of Molecular Biology, Faculty of Chemistry, Autonomous University of Nuevo León, Nuevo León, Mexico; 4Colloidal Materials Research Group, Institute of Materials Science of Seville, Spanish National Research Council, University of Seville, Seville, Spain Abstract: Coriander leaves and seeds have been highly appreciated since ancient times, not only due to their pleasant flavors but also due to their inhibitory activity on food degradation and their beneficial properties for health, both ascribed to their strong antioxidant activity. Recently, it has been shown that coriander leaf extracts can mediate the synthesis of metallic nanoparticles through oxidation/reduction reactions. In the present study, extracts of coriander leaves and seeds have been used as reaction media for the wet chemical synthesis of ultrafine silver nanoparticles and nanoparticle clusters, with urchin- and tree-like shapes, coated by biomolecules (mainly, proteins and polyphenols. In this greener route of nanostructure preparation, the active biocompounds of coriander simultaneously play the roles of reducing and stabilizing agents. The morphological and microstructural studies of the resulting biosynthesized silver nanostructures revealed that the nanostructures prepared with a small concentration of the precursor Ag salt (AgNO3 =5 mM exhibit an ultrafine size and a narrow size distribution, whereas particles synthesized with high concentrations of the precursor Ag salt (AgNO3 =0.5 M are polydisperse and formation of supramolecular structures occurs. Fourier transform infrared and Raman spectroscopy

  3. Pea (Pisum sativum L.) in the genomics era

    Science.gov (United States)

    Pea (Pisum sativum L.) was the original model organism for Mendel´s discovery of the laws of inheritance, making it the foundation of modern plant genetics. However, subsequent progress in pea genomics has lagged behind many other plant species, largely as a consequence of its low multiplication rat...

  4. 不同漂烫时间对芫荽营养成分的影响%The Effect of Different Blanching Time on Nutrients of Coriander

    Institute of Scientific and Technical Information of China (English)

    陈蔚辉; 徐佳霖

    2015-01-01

    采用不同漂烫时间对芫荽进行漂烫处理,测定处理后芫荽各种营养成分的变化,评价芫荽的最佳漂烫时间。结果表明:芫荽对漂烫处理反应灵敏,随着漂烫时间的延长,芫荽的可溶性糖、蛋白质、叶绿素、有机酸和维生素 C 等营养成分含量均呈现不同程度的降低。仅从对芫荽营养成分保持程度评价最佳漂烫时间,则保持叶绿素、有机酸和维生素 C 含量的最佳漂烫时间均为10 s,可溶性糖和蛋白质则为30 s。%Coriander is blanched with different blanching time to measure various nutrients changes of coriander after treatment and evaluate the best blanching time of coriander.The results show that coriander is responsive to the blanching treatment and with the extension of blanching time,the content of soluble sugar,protein,chlorophyll,organic acid,and Vitamin C of coriander all show different degrees of decline.If choose the best blanching time to maintain the optimum nutrition of coriander,10 s is the best for chlorophyll,organic acid and Vitamin C,while 30 s is the best for protein and soluble sugar.

  5. Postharvest Processing and Benefits of Black Pepper, Coriander, Cinnamon, Fenugreek, and Turmeric Spices.

    Science.gov (United States)

    Balasubramanian, S; Roselin, P; Singh, K K; Zachariah, John; Saxena, S N

    2016-07-26

    Spices are prime source for flavor, aroma, and taste in cuisines and play an active role as medicines due to their high antioxidant properties. As medicine or food, the importance of spices cannot be overemphasized. The medicinal values of spices are very well established in treating various ailments like cancer, fever, malaria, stomach offset, nausea, and many more. A spice may be available in several forms: fresh, whole dried, or pre-ground dried which requires further processing to be utilized in the form of value-added product. This review paper deals with the cultivation, postharvesting, chemical composition, uses, health, and medicinal benefits of the selected spice viz., black pepper, coriander, cinnamon, fenugreek, turmeric, and technological advances in processing of spices viz., super critical fluid extraction, cryogenic grinding, and microencapsulation etc. This paper also focuses on issues related to utilization of spices toward its high end-product development and characterization in pharmaceuticals and other medicinal purposes. The availability of different spices and their varietal differences and location have their pertinent characters, which are much demanding to refine postharvest and processing to assure its quality in the international market.

  6. 香菜热风干燥的试验研究%Experimental Study on Hot Air Drying of Coriander

    Institute of Scientific and Technical Information of China (English)

    赵昆; 赵士杰; 滕竹竹

    2016-01-01

    首先通过试验分析了漂烫预处理对香菜热风干燥特性的影响,然后用单因素试验设计方法探讨热风温度、热风速度和铺料层厚度对香菜热风干燥特性的影响,之后又对香菜干制品进行复水试验,得出了香菜干制品在不同干燥条件下的复水特性.结果表明:热风温度越高,热风速度越大,铺料层厚度越小,干燥速率越大,干燥过程所需时间越短;但是,过高的热风温度、热风速度和过小的铺料层厚度都会降低香菜干制品的复水性能.%In this paper, the effect of blanching pretreatment on drying characteristics of coriander was investigated through the experiment.The impacts of hot air temperature, hot air velocity and thickness of materials on the hot air dr-ying characteristics of coriander were investigated by single factor experiment design, and the rehydration test further ob-tained the rehydration characteristics of dried materials under different drying conditions.The results show that higher hot air temperature, faster hot air velocity and smaller thickness of the material will cause greater drying rate and shorten the whole drying time.However, too high hot air temperature, hot air velocity and too small thickness of the material layer can reduce the rehydration capacity of the dried coriander.

  7. Comparative performance of coriander dryer coupled to solar air heater and solar air-heater-cum-rockbed storage

    Energy Technology Data Exchange (ETDEWEB)

    Chauhan, P.M.; Choudhury, C.; Garg, H.P. [Indian Inst. of Technology, Centre for Energy Studies, New Delhi (India)

    1996-03-01

    In the present communication, efforts have been made to study the drying characteristics of coriander in a stationary 0.5 tonne/batch capacity deep-bed dryer coupled to a solar air heater and a rockbed storage unit to receive hot air during sunshine and off-sunshine hours, respectively. The drying bed was assumed to consist of a number of thin layers of grains stacked upon each other. The theoretical investigation was made by writing the energy and mass balance equations for different components of the dryer-cum-air-heater-cum-storage and by adopting a finite difference approach for simulation. (author)

  8. ANTIOXIDANT ACTIVITY OF WINTER SLAVONIAN GARLIC (Allium sativum L.)

    OpenAIRE

    2012-01-01

    Numerous studies conducted on garlic (Allium sativum L.), have proved the presence of antioxidants, phenolic compounds, sulfur compounds and several vitamins. The aim of this study was to investigate the differences in the content of total phenols, ascorbic acid; total antioxidant activities of four winter Slavonian garlic cultivars, as well as establishment of correlations between these parameters. The total antioxidant activity and the content of ascorbic acid and total phenols in four cult...

  9. 复方新诺明(SMZ-TMP)对芫荽(Coriandrum stativum)种子萌发和幼苗生长的影响%Effects Of Sulfamethoxazolum-Trimethoprimum (SMZ-TMP) on the Seed Germination and Seedling Growth of Coriander (Coriandrum Srativum)

    Institute of Scientific and Technical Information of China (English)

    刘永军; 赵宗芸; 杨晓玲; 李静芳

    2003-01-01

    不同浓度的复方新诺明溶液对芫荽种子的发芽率无明显影响,但对其发芽势影响较大,除2.00mg·L~(-1)处理外,其余各处理较对照都有极显著提高。对苗高、苗重及根长、根重和蛋白质、叶绿素、可溶性糖含量均有显著提高。以0.5mg·L~(-1)处理效果最好。

  10. Effect of coriander seed powder (CSP) on 1, 2-dimethyl hydrazine-induced changes in antioxidant enzyme system and lipid peroxide formation in rats.

    Science.gov (United States)

    Anilakumar, K R; Khanum, Farhath; Bawa, A S

    2010-03-01

    The effect of coriander seed powder (CSP), a culinary spice, on dimethyl hydrazine (DMH)-induced oxidative stress and toxicity in rats was investigated. Six groups of 6 male rats each were maintained for 12 weeks as (a) Control; (b) DMH (60 mg/kg body weight) injected; (c) 5% CSP incorporated diet; (d) 5% CSP incorporated diet + DMH; (e) 10% CSP incorporated diet; and (f) 10% CSP incorporated diet + DMH. The rats were sacrificed after 12 weeks. The results revealed that DMH administration lead to an increase in hepatic lipid peroxidation associated with reduction in levels of glutathione (GSH), activity of superoxide dismutase (SOD), and catalase and glucose-6-phosphate dehydrogenase. The coadministration of CSP and DMH diminished the hepatic malondialdehyde (MDA) significantly as compared to DMH-alone administered rats. The intake of coriander seeds at 10% level also enhanced the hepatic GSH-redox system by elevating GSH-Px, GSSGR, and GST activities. The DMH-induced decline in SOD and catalase activities was brought to normal by 10% CSP. The coadministration of CSP and the DMH produced a significant reduction in MDA and enhancement in catalase activity as compared to control. Coriander powder at 5% and 10% levels produced a significant rise in colonic catalase and GSH-Px. The coriander seeds produced significant beneficial effects by reducing the DMH-induced oxidative stress and enhancing the tissue levels of antioxidant/detoxification agent in tissues.

  11. Studies on the Bacteriostatic Effects of Water Extraction liquid from Coriander%芫荽水提液对细菌的抑制作用研究

    Institute of Scientific and Technical Information of China (English)

    杨霞

    2012-01-01

    To study the bacteriostatic effects of water extraction liquid from Coriander, fresh Coriander was washed,dried,and sterilized by UV exposure. E. coli and Bacillus subtilis were used as the tested strains and the experiment was performed by the method of filter patch. The results showed that the Coriander water extraction liquid had different degrees of bacteriostasis to the two tested strains and the bacteriostatic circle size of water extraction liquid from Coriander were different between the two tested strains. The bacteriostasis was enhanced accompanied with the increasing of Coriander water extraction liquid concentration.%为了明确芫荽水提液对细菌的抑制作用,将新鲜芫荽洗净、晾干,紫外线杀菌处理后研碎,以大肠杆菌和枯草芽孢杆菌为供试菌种,采用滤纸片贴片法对芫荽水提液的抑菌作用进行了研究,供试液分别为25%、50%、75%、100%的芫荽提取液.结果表明,芫荽水提液对以上2种供试菌种所产生的抑菌圈大小不同,不同体积分数的芫荽水提液对2种菌有不同程度的抑菌作用.随着芫荽水提液体积分数的增大,抑菌作用增强,这说明芫荽水提取液对细菌有抑菌活性.

  12. PHENOLIC PROFILE, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES FROM THE Apiaceae FAMILY (DRY SEEDS

    Directory of Open Access Journals (Sweden)

    Valentina Lubomirova Christova-Bagdassarian

    2013-11-01

    Full Text Available Objective: In the present study, a comparative evaluation of the phenolic compounds antioxidant capacity and antibacterial activity to several kinds of seeds from the Apiacea family, to which belong: Foeniculum vulgare (Fennel, Anethum graveolens (Dill, Pimpinella anisum (Anise, Carum carvi (Caraway and Coriandrum sativum (Coriander were carried out. Methods: The total phenolic content of seeds was measured spectrophotometrically by using the Folin-Ciocalteu assay, the total flavonoids was measured spectrophotometrically by using the aluminum chloride colorimetric. Antioxidant capacity was analysed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH radical scavenging effect of the seeds and was determined also spectrophotometrically. Antibacterial activity was analysed by ISO standards. Results: The total phenolic and flavonoid contents of the Foeniculum vulgare (Fennel varied between 115.96 mg GAE/100g and 68.10 mg CE/100g .The Coriandrum sativum (Coriander content is lower (from 17.04 mg GAE/100g to 11.10 mg CE/100g, respecively. The highest radical scavanging effect was observed in the Foeniculum vulgare (Fennel with IC50 of 113.19 ml/L. In our study, the methanol extract of seeds didn’t have any antimicrobial activity. Conclusions: An original data for total phenolic and total flavonoid contents are present in this study. They are a basis for assessment of the role of Apiaceae family dry seeds against free radicals effect and antibacterial activity. The results show that methanolic extract has the highest of total phenolic and total flavonoid contents, high potential of antioxidant activity of dry seeds from Apiaceae family, to which belong: Fennel (Foeniculum vulgare, Dill (Anethum graveolens, Anise (Pimpinella anisum, Caraway (Carum carvi and Coriander (Coriandrum sativum. From all results, the solvent extracts of dry seeds are rich in phytochemical contents, which possessed high antioxidant and antimicrobial activities. Therefore the data found in

  13. 香菜的平衡含水率测定试验研究%Experimental Study on Determination of Equilibrium Moisture Content of Coriander

    Institute of Scientific and Technical Information of China (English)

    赵昆; 赵士杰; 滕竹竹

    2016-01-01

    In this paper , moisture absorption and desorption equilibrium moisture content of coriander were measured by the static method and the testing device of the DL104 type electric heating and air drying box while the environmental relative hu-midity changed from 10%to 95 %and the temperature was at different values such as 25℃and 35℃.The moisture desorption and absorption isotherms of coriander were plotted , the effects of temperature and environmental re-lative humidity on the equi-librium moisture content of coriander were analyzed and the safe storage condition of dried coriander was determined .The rus-ults show that the equilibrium moisture content of coriander exists moisture absorp-tion "lagging"phenomenon , the envir-onmental relative humidity has more important influence than temperature on the equilibrium moisture content of corian-der , the mildew phenomena will appear when the dry base moisture content of the sample was greater than 50%and the mil-dew phenomena will become serious when the environmental relative humidity is greater than 60%.%利用DL104 型电热鼓风干燥箱试验装置,采用静态法分别测得香菜在温度为25℃和35℃、相对湿度为10%~95%范围内的解吸和吸湿平衡含水率数据,绘制了相应的香菜解吸和吸湿等温线,分析了温度和环境相对湿度对香菜平衡含水率的影响,确定了香菜干制品的安全贮藏条件. 结果表明:香菜的平衡含水率存在吸湿"滞后"现象;环境相对湿度比温度对香菜平衡含水率的影响更大;当香菜样品干基含水率大于50%时,出现霉变现象;当环境相对湿度大于60%时,霉变现象严重.

  14. Isolation and Characterization of Novel EST-Derived Genic Markers in Pisum sativum (Fabaceae

    Directory of Open Access Journals (Sweden)

    Shalu Jain

    2013-10-01

    Full Text Available Premise of the study: Novel markers were developed for pea (Pisum sativum from pea expressed sequence tags (ESTs having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. Methods and Results: Seventy-seven EST-derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. Conclusions: The novel markers described here will be useful for future genetic studies of P. sativum; their amplification in lentil (Lens culinaris demonstrates their potential for use in closely related species.

  15. Research progress in functional ingredient and healthy function of coriander%芫荽的成分及保健功能的研究进展

    Institute of Scientific and Technical Information of China (English)

    赵秀玲

    2011-01-01

    Coriander is a plant with high health protection. It has special flavor and nourishing and multiple bioactivities, such as antibalterial, anticancer, antioxidant activities etc. The chemical composition, functional ingredient and health-protections functions of coriander were introduced in order to make of this plant.%芫荽是一种具有较高的食用和药用的植物资源,风味独特,营养丰富,保健功能强,含有多种对人体有益的活性物质,具有抗菌、抗肿瘤、抗氧化等保健作用,这些特性使芫荽得到广泛关注.本文介绍了芫荽的化学成分、功效成分和保健作用,旨在促进芫荽开发利用.

  16. Determination of Cadmium, Lead and Zinc in Vegetables in Jaipur (India).

    Science.gov (United States)

    Kumar, Ashok; Verma, P S

    2014-01-01

    An atomic absorption spectroscopic method was used for the determination of Lead, Cadmium and Zinc in vegetables grown in and around Jaipur food stuffs irrigated with industrial waste water. Vegetable samples were collected after maturity, and analyzed, such as spinach (Spinacia oleracea), ladyfinger (Abelmoschus esulentus), pepper mint (Menthe pipereta), brinjal (Solanum melongena), coriander (Coriandrum sativum), cauliflower (Brassica oleracea), onion (Allium cepa), radish (Raphanus sativus), pointedgourd (Trichosanthes dioica), bottlegourd (Lagenaria siceraria), chilies (Capsicum annum), ribbedgourd (Luffa acutangula) and pumpkin (Curcurbites pepo). The concentration of Lead ranged between 1.40-71.06 ppm, Cadmium 0.61-34.48 ppm and Zinc 0.39-187.26 ppm in vegetable samples. The results reveal that urban consumers are at greater risk of purchasing fresh vegetables with high levels of heavy metal, beyond the permissible limits, as defined by the Indian Prevention of Food Adulteration Act, 1954 and WHO.

  17. Modulation of the multidrug efflux pump EmrD-3 from Vibrio cholerae by Allium sativum extract and the bioactive agent allyl sulfide plus synergistic enhancement of antimicrobial susceptibility by A. sativum extract.

    Science.gov (United States)

    Bruns, Merissa M; Kakarla, Prathusha; Floyd, Jared T; Mukherjee, Mun Mun; Ponce, Robert C; Garcia, John A; Ranaweera, Indrika; Sanford, Leslie M; Hernandez, Alberto J; Willmon, T Mark; Tolson, Grace L; Varela, Manuel F

    2017-04-21

    The causative agent of cholera, Vibrio cholerae, is a public health concern. Multidrug-resistant V. cholerae variants may reduce chemotherapeutic efficacies of severe cholera. We previously reported that the multidrug efflux pump EmrD-3 from V. cholerae confers resistance to multiple structurally distinct antimicrobials. Medicinal plant compounds are potential candidates for EmrD-3 efflux pump modulation. The antibacterial activities of garlic Allium sativum, although poorly understood, predicts that a main bioactive component, allyl sulfide, modulates EmrD-3 efflux. Thus, we tested whether A. sativum extract acts in synergy with antimicrobials and that a main bioactive component allyl sulfide inhibits EmrD-3 efflux. We found that A. sativum extract and allyl sulfide inhibited ethidium bromide efflux in cells harboring EmrD-3 and that A. sativum lowered the MICs of multiple antibacterials. We conclude that A. sativum and allyl sulfide inhibit EmrD-3 and that A. sativum extract synergistically enhances antibacterial agents.

  18. A multicentric, double-blind randomized, homoeopathic pathogenetic trial of Allium sativum

    Directory of Open Access Journals (Sweden)

    Raj K Manchanda

    2016-01-01

    Conclusion: The pathogenetic response elicited during the proving trial expands the scope of use of the drug A. sativum and will benefit the research scholars and clinicians. The generated symptoms of this drug will carry more value when verified clinically.

  19. The Effect of Allium Sativum and Xylopia Aethiopica Extracts on the ...

    African Journals Online (AJOL)

    BSN

    Department Of Science Laboratory Technology, The Federal Polytechnic, Bida. P.M.B.55, Bida ... chilling injury at temperatures from 00C to 100C. (Ihenkoronye ... sativum and fruits of Xylopia aethiopica were .... Nigerian Journal of Applied.

  20. A review on the effects of Allium sativum (Garlic) in metabolic syndrome.

    Science.gov (United States)

    Hosseini, A; Hosseinzadeh, H

    2015-11-01

    The metabolic syndrome is a common problem world-wide and includes abdominal obesity, hypertension, dyslipidemia, and hyperglycemia disorders. It leads to insulin resistance and the development of diabetes mellitus or cardiovascular disease. Allium sativum (garlic) has been documented to exhibit anti-diabetic, hypotensive, and hypolipidemic properties. This suggests a potential role of A. sativum in the management of metabolic syndrome; however, more studies should be conducted to evaluate its effectiveness. In this review, we discussed the most relevant articles to find out the role of A. sativum in different components of metabolic syndrome and cardiovascular disease risk factors. Because human reports are rare, further studies are required to establish the clinical value of A. sativum in metabolic syndrome.

  1. Studies on antimicrobial activities of solvent extracts of different spices.

    Science.gov (United States)

    Keskin, Dilek; Toroglu, Sevil

    2011-03-01

    The antimicrobial activities of the ethyl acetate, acetone and methanol extract of 12 plant species were studied. The extract of Capsicum annuum (red pepper) (fruit) Zingiber officinale (ginger) (root), Cuminum cyminum (cumin), Alpinia ficinarum (galingale), Coriandrum sativum (coriander), Cinnamomun zeylanicum Nees (cinnamomun), Origanum onites L. (thyme), Folium sennae (senna), Eugenia caryophyllata (cloves), Flos tiliae (lime), Folium menthae crispae (peppermint) and Piper nigrum (blackpepper) were tested in vitro against 2 fungi and 8 bacterial species by the disc diffusion method. Klebsiella pneumonia 13883, Bacillus megaterium NRS, Pseudomonas aeroginosa ATCC 27859, Staphylococcus aureus 6538 P, Escherichia coil ATCC 8739, Enterobacter cloaca ATCC 13047, Corynebacterium xerosis UC 9165, Streptococcus faecalis DC 74, Kluyveromyces marxianus, Rhodotorula rubra were used in this investigation. The results indicated that extracts of different spices has shown antibacterial activity in the range of 7-24 mm 30 microl(-1) inhibition zone Eugenia caryophyllata (clove), 7-20 mm 30microl(-1) inhibition zone Capsicum annum (red pepper) and Cinnamomun zeylanicum (cinnamon) bark, 7-18 mm 30microl(-1) inhibition zone Folium sennae (senna) leaves, 7-16 mm 30 microl(-1) inhibition zone Zingiber officinale (ginger) root, 7-15 mm 30microl(-1) inhibition zone Cuminum cyminum (cumin) seed, 7-14 mm 30 microl(-1) inhibition zone Folium menthae crispae (peppermint), Origanum onites (thyme) leaves and Alpinia ficinarum (galingale) root, 7-12 mm 30 microl(-1) inhibiton zone Piper nigrum (blackpepper), 7-11 mm 30microl(-1) inhibition zone Flos tiliae (lime) leaves, 7-8 mm 30microl(-1) inhibition zone Coriandrum sativum (coriander) to the microorganisms tested.

  2. Garlic (Allium sativum L.: A review of potential therapeutic applications

    Directory of Open Access Journals (Sweden)

    Prasan R Bhandari

    2012-01-01

    Full Text Available Garlic (Allium sativum L. fam. Alliaceae, one of the best-researched herbal remedies, is frequently used as a food and a spice. Garlic contains enzymes (e.g., allinase, sulphur-containing compounds, including alliin, and compounds produced enzymatically from alliin (for example, allicin. Conventionally, it has been used to treat infections, wounds, diarrhoea, rheumatism, heart disease, diabetes and many other disorders. Experimental studies have demonstrated that garlic exhibits antibacterial, antihypertensive, cardioprotective, antilipidaemic, anticarcinogenic, immunostimulant and hypoglycaemic properties. Clinically, garlic has been investigated for a variety of indications, namely, hypertension, hypercholesterolaemia, diabetes and for the prevention of arteriosclerosis and cancer. Systematic reviews are available for the possible antilipidaemic, antihypertensive, antithrombotic and chemopreventive effects. Garlic appears to be generally safe although allergic reactions may occur.

  3. Micropropagation and cryopreservation of garlic (Allium sativum L.).

    Science.gov (United States)

    Keller, E R Joachim; Senula, Angelika

    2013-01-01

    Garlic (Allium sativum L.) is a very important medicinal and spice plant. It is conventionally propagated by daughter bulbs ("cloves") and bulbils from the flower head. Micropropagation is used for speeding up the vegetative propagation mainly using the advantage to produce higher numbers of healthy plants free of viruses, which have higher yield than infected material. Using primary explants from bulbs and/or bulbils (shoot tips) or unripe inflorescence bases, in vitro cultures are initiated on MS-based media containing auxins, e.g., naphthalene acetic acid, and cytokinins, e.g., 6-γ-γ-(dimethylallylaminopurine) (2iP). Rooting is accompanying leaf formation. It does not need special culture phases. The main micropropagation methods rely on growth of already formed meristems. Long-term storage of micropropagated material, cryopreservation, is well-developed to maintain germplasm. The main method is vitrification using the cryoprotectant mixture PVS3.

  4. Effect of dietary supplement of sugar beet, neem leaf, linseed and coriander on growth performance and carcass trait of Vanaraja chicken

    Directory of Open Access Journals (Sweden)

    Punita Kumari

    2014-09-01

    Full Text Available Aim: This study was planned to investigate the effect of sugar beet, neem leaf, linseed and coriander on growth parameters such as feed intake, body weight gain, feed conversion ratio (FCR, performance index (PI, and carcass characteristics in broiler birds. Materials and Methods: The experiment was conducted for a period of 42 days on Vanaraja strain of broiler birds. Different dietary supplement such as sugar beet meal, neem leaf meal, linseed meal and coriander seed meal were used in the basal diet. All day-old 150 male chicks were individually weighed and distributed into five groups having 30 birds in each. Each group was further sub-divided into triplicates having 10 birds in each. Group T1served as control and rest groups T2, T3, T4 and T5 as treatment groups. Birds in T1 group were fed basal ration only, however, T2 , T3, T4 and T5 groups were fed basal ration mixed with 2.5% sugar beet meal, neem leaf meal, linseed meal, and coriander seed meal individually, respectively. Results: Broilers supplemented with herbs/spices showed improvement in growth attributes and carcass characteristics. Broilers fed with herbs at the rate of 2.5% had higher feed intake except sugar beet and coriander seed meal fed group. The body weight and weight gain was also significantly (p0.05 affected. Average giblet percentage of all supplemented groups were significantly (p<0.05 higher than control and was found to be highest in neem leaf meal fed group. Average by-product percentage was found to be highest in linseed fed group. Conclusion: Various herbs such as sugar beet, neem leaf, linseed and coriander seed meals affected the growth performance, and carcass trait showed positive inclination toward supplemented groups in broilers. The exact mode of action of these herbs/spices is still not clear, however, one or more numbers of active compounds present in these supplements may be responsible.

  5. Green synthesis of multifunctional carbon dots from coriander leaves and their potential application as antioxidants, sensors and bioimaging agents.

    Science.gov (United States)

    Sachdev, Abhay; Gopinath, P

    2015-06-21

    In the present study, a facile one-step hydrothermal treatment of coriander leaves for preparing carbon dots (CDs) has been reported. Optical and structural properties of the CDs have been extensively studied by UV-visible and fluorescence spectroscopic, microscopic (transmission electron microscopy, scanning electron microscopy) and X-ray diffraction techniques. Surface functionality and composition of the CDs have been illustrated by elemental analysis and Fourier transform infrared spectroscopy (FTIR). Quenching of the fluorescence of the CDs in the presence of metal ions is of prime significance, hence CDs have been used as a fluorescence probe for sensitive and selective detection of Fe(3+) ions. Eventually, biocompatibility and bioimaging aspects of CDs have been evaluated on lung normal (L-132) and cancer (A549) cell lines. Qualitative analysis of cellular uptake of CDs has been pursued through fluorescence microscopy, while quantitative analysis using a flow cytometer provided an insight into the concentration and cell-type dependent uptake of CDs. The article further investigates the antioxidant activity of CDs. Therefore, we have validated the practicality of CDs obtained from a herbal carbon source for versatile applications.

  6. Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice

    Directory of Open Access Journals (Sweden)

    Gamboa-Leon Rubi

    2014-01-01

    Full Text Available We tested a mixture of Tridax procumbens, known for its direct action against Leishmania mexicana, and Allium sativum, known for its immunomodulatory effect, as an alternative to treat cutaneous leishmaniasis. Acute oral toxicity was tested with the Up-and-Down Procedure (UDP using a group of healthy mice administered with either T. procumbens or A. sativum extracts and compared with a control group. Liver injury and other parameters of toxicity were determined in mice at day 14. The in vivo assay was performed with mice infected with L. mexicana promastigotes and treated with either a mixture of T. procumbens and A. sativum or each extract separately. The thickness of the mice’s footpads was measured weekly. After the 12-week period of infection, blood samples were obtained by cardiac puncture to determine the total IgG, IgG1 and IgG2a immunoglobulins by a noncommercial indirect ELISA. We showed that the mixture of T. procumbens and A. sativum extracts was better at controlling L. mexicana infection while not being toxic when tested in the acute oral toxicity assay in mice. An increase in the ratio of IgG2a/IgG1 indicated a tendency to raise a Th1-type immune response in mice treated with the mixture. The mixture of T. procumbens and A. sativum extracts is a promising natural treatment for cutaneous leishmaniasis and its healing effects make it a good candidate for a possible new phytomedicine.

  7. The Chemical Compositions of the Volatile Oils of Garlic (Allium sativum and Wild Garlic (Allium vineale

    Directory of Open Access Journals (Sweden)

    Prabodh Satyal

    2017-08-01

    Full Text Available Garlic, Allium sativum, is broadly used around the world for its numerous culinary and medicinal uses. Wild garlic, Allium vineale, has been used as a substitute for garlic, both in food as well as in herbal medicine. The present study investigated the chemical compositions of A. sativum and A. vineale essential oils. The essential oils from the bulbs of A. sativum, cultivated in Spain, were obtained by three different methods: laboratory hydrodistillation, industrial hydrodistillation, and industrial steam distillation. The essential oils of wild-growing A. vineale from north Alabama were obtained by hydrodistillation. The resulting essential oils were analyzed by gas chromatography-flame ionization detection (GC-FID and gas chromatography-mass spectrometry (GC-MS. Both A. sativum and A. vineale oils were dominated by allyl polysulfides. There were minor quantitative differences between the A. sativum oils owing to the distillation methods employed, as well as differences from previously reported garlic oils from other geographical locations. Allium vineale oil showed a qualitative similarity to Allium ursinum essential oil. The compositions of garlic and wild garlic are consistent with their use as flavoring agents in foods as well as their uses as herbal medicines. However, quantitative differences are likely to affect the flavor and bioactivity profiles of these Allium species.

  8. Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice.

    Science.gov (United States)

    Gamboa-Leon, Rubi; Vera-Ku, Marina; Peraza-Sanchez, Sergio R; Ku-Chulim, Carlos; Horta-Baas, Aurelio; Rosado-Vallado, Miguel

    2014-01-01

    We tested a mixture of Tridax procumbens, known for its direct action against Leishmania mexicana, and Allium sativum, known for its immunomodulatory effect, as an alternative to treat cutaneous leishmaniasis. Acute oral toxicity was tested with the Up-and-Down Procedure (UDP) using a group of healthy mice administered with either T. procumbens or A. sativum extracts and compared with a control group. Liver injury and other parameters of toxicity were determined in mice at day 14. The in vivo assay was performed with mice infected with L. mexicana promastigotes and treated with either a mixture of T. procumbens and A. sativum or each extract separately. The thickness of the mice's footpads was measured weekly. After the 12-week period of infection, blood samples were obtained by cardiac puncture to determine the total IgG, IgG1 and IgG2a immunoglobulins by a noncommercial indirect ELISA. We showed that the mixture of T. procumbens and A. sativum extracts was better at controlling L. mexicana infection while not being toxic when tested in the acute oral toxicity assay in mice. An increase in the ratio of IgG2a/IgG1 indicated a tendency to raise a Th1-type immune response in mice treated with the mixture. The mixture of T. procumbens and A. sativum extracts is a promising natural treatment for cutaneous leishmaniasis and its healing effects make it a good candidate for a possible new phytomedicine.

  9. Preclinical Evaluation of Hepatoprotective Activity of Ocimum basilicum L. and Allium sativum L.

    Directory of Open Access Journals (Sweden)

    Deodelsy Bermúdez Toledo

    2014-02-01

    Full Text Available Background: finding natural treatments designed to protect the liver from the damaging effects of hepatotoxins is an important topic in medical and pharmaceutical research. Objective: to pre-clinically evaluate the hepatoprotective activity of the species Ocimum basilicum L. and Allium sativum L. in an animal model of acetaminophen-induced toxicity. Methods: a preclinical pharmacological study was conducted to evaluate the hepatoprotective effect of the species Ocimum basilicum L. and Allium sativum L. against acetaminophen-induced toxicity. Adult male NMRI mice were used. They were orally administered soft extracts of the plants at doses of 200mg/kg and 400mg/kg for three consecutive days before inducing hepatotoxicity. Clinical signs of toxicity and hepatic biochemical parameters were evaluated; in addition, the morphological analysis of the liver was performed. Results: the biochemical parameters showed highly significant differences, but only three groups had results similar to the untreated control group. No macroscopic changes in the liver were confirmed. Microscopically, the absence of histopathological changes prevailed in the groups treated with Ocimum basilicum L. at both doses and Allium sativum L. at 200mg/kg; slight damage was observed by administering 400mg/kg of Allium sativum L., with significant differences from the untreated control group. Conclusions: soft extracts of the species Ocimum basilicum L. and Allium sativum L. at a dose of 200mg/kg have greater hepatoprotective activity against the toxic action of acetaminophen.

  10. Antimycobacterial and Antibacterial Activity of Allium sativum Bulbs.

    Science.gov (United States)

    Viswanathan, V; Phadatare, A G; Mukne, Alka

    2014-05-01

    Tuberculosis is one of the major public health problems faced globally. Resistance of Mycobacterium tuberculosis to antitubercular agents has called for an urgent need to investigate newer drugs to combat tuberculosis. Garlic (Allium sativum) is an edible plant which has generated a lot of curiosity throughout human history as a medicinal plant. Garlic contains sulfur compounds like allicin, ajoene, allylmethyltrisulfide, diallyltrisulfide, diallyldisulphide and others which exhibit various biological properties like antimicrobial, anticancer, antioxidant, immunomodulatory, antiinflammatory, hypoglycemic, and cardiovascular effects. According to various traditional systems of medicine, garlic is one of the established remedies for tuberculosis. The objective of the current study was to investigate in vitro antimycobacterial activity as well as anti-bacterial activity of various extracts rich in specific phytoconstituents from garlic. Preparation of garlic extracts was done based on the chemistry of the constituents and their stability. The estimation of in vitro antimycobacterial activity of different garlic extracts was done using Resazurin microtire plate assay technique whereas activity of garlic oil was evaluated by colony count method. The antibacterial activity of extracts and oil was estimated by zone of inhibition method. Extracts of garlic rich in allicin and ajoene showed appreciable antimycobacterial activity as compared to standard drugs. Garlic oil demonstrated significant antibacterial activity, particularly against methicillin-resistant Staphylococcus aureus.

  11. EDTA and urease effects on Hg accumulation by Lepidium sativum.

    Science.gov (United States)

    Smolińska, Beata; Cedzyńska, Krystyna

    2007-11-01

    The phytoextraction process was conducted under laboratory conditions with the use of garden cress plants (Lepidium sativum). The experiment was carried out in a model soil, which was characterized before conducting the process. Inorganic forms of mercury (HgCl(2), HgSO(4), Hg(NO(3))(2)) were used for contamination of the soil. The phytoextraction process was conducted after EDTA application to the soil and after urease application. Also the influence of simultaneous addition of ethylenediaminetetraacetic acid (EDTA) and urease into the soil on phytoextraction process was measured. In all variants of phytoextraction process the total mercury concentrations in roots, stems and leaves of garden cress were determined. The result showed that garden cress accumulated mercury from soil. The overall maximum concentration of mercury in its compounds was found in roots of the plant. In all cases, before addition of urease and EDTA, the translocation process and distribution of mercury in the plant tissues were limited. The addition of urease caused an increase of enzyme activity in the soil and at the same time caused an increase of mercury concentration in plant tissues. Application of EDTA increased solubility of mercury and caused an increase of metal accumulation by plants. After simultaneous addition of EDTA and urease into the soil garden cress accumulated about 20% of total mercury concentration in the soil. Most of mercury compounds were accumulated in leaves and stems of the plants (46.0-56.9% of total mercury concentration in the plant tissues).

  12. Antimycobacterial and antibacterial activity of Allium sativum bulbs

    Directory of Open Access Journals (Sweden)

    V. Viswanathan

    2014-01-01

    Full Text Available Tuberculosis is one of the major public health problems faced globally. Resistance of Mycobacterium tuberculosis to antitubercular agents has called for an urgent need to investigate newer drugs to combat tuberculosis. Garlic (Allium sativum is an edible plant which has generated a lot of curiosity throughout human history as a medicinal plant. Garlic contains sulfur compounds like allicin, ajoene, allylmethyltrisulfide, diallyltrisulfide, diallyldisulphide and others which exhibit various biological properties like antimicrobial, anticancer, antioxidant, immunomodulatory, antiinflammatory, hypoglycemic, and cardiovascular effects. According to various traditional systems of medicine, garlic is one of the established remedies for tuberculosis. The objective of the current study was to investigate in vitro antimycobacterial activity as well as anti-bacterial activity of various extracts rich in specific phytoconstituents from garlic. Preparation of garlic extracts was done based on the chemistry of the constituents and their stability. The estimation of in vitro antimycobacterial activity of different garlic extracts was done using Resazurin microtire plate assay technique whereas activity of garlic oil was evaluated by colony count method. The antibacterial activity of extracts and oil was estimated by zone of inhibition method. Extracts of garlic rich in allicin and ajoene showed appreciable antimycobacterial activity as compared to standard drugs. Garlic oil demonstrated significant antibacterial activity, particularly against methicillin-resistant Staphylococcus aureus.

  13. Evaluation of SRAP markers for mapping of Pisum sativum L.

    Directory of Open Access Journals (Sweden)

    María Fernanda Guindon

    2016-09-01

    Full Text Available Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 was generated. A total of 25 SRAP primer combinations were evaluated in 45 F2 plants and both parental lines, generating 208 polymorphic bands/markers. The markers were analyzed by the chi-square goodness-of-fit test to check the expected Mendelian segregation ratio. The resulting linkage map consists of 112 genetic markers distributed in 7 linkage groups (LGs, covering a total of 528.8 cM. The length of the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM, with 9 to 34 markers. The linkage map developed in this study indicates that the SRAP marker system could be applied to mapping studies of pea.

  14. Studies on the mitotic chromosome scaffold of Allium sativum

    Institute of Scientific and Technical Information of China (English)

    ZHAOJIAN; SHAOBOJIN; 等

    1995-01-01

    An argentophilic structure is present in the metaphase chromosomes of garlic(Allium sativum),Cytochemical studies indicate that the main component of the structure is non-histone proteins(NHPs).The results of light and electron microscopic observations reveal that the chromosme NHP scaffold is a network which is composed of fibres and granules and distributed throughout the chromosomes.In the NHP network,there are many condensed regions that are connected by redlatively looser regions.The distribution of the condensed regions varies in individual chromosomes.In some of the chromosomes the condensed regions are lognitudinally situsted in the central part of a chromatid while in others these regions appear as coillike transverse bands.At early metaphase.scaffolds of the sister chromatids of a chromosome are linked to each other in the centromeric region,meanwhile,they are connected by scafold materials along the whole length of the chromosome.At late metaphase,however,the connective scaffold materials between the two sister chromatids disappear gradually and the chromatids begin to separate from one another at their ends.but the chromatids are linked together in the centromeric region until anaphase.This connection seems to be related to the special structure of the NHP scaffold formed in the centromeric region.The morphological features and dynamic changes of the chromosome scaffold are discussed.

  15. ANTIOXIDANT ACTIVITY OF WINTER SLAVONIAN GARLIC (Allium sativum L.

    Directory of Open Access Journals (Sweden)

    Nada Parađiković

    2012-12-01

    Full Text Available Numerous studies conducted on garlic (Allium sativum L., have proved the presence of antioxidants, phenolic compounds, sulfur compounds and several vitamins. The aim of this study was to investigate the differences in the content of total phenols, ascorbic acid; total antioxidant activities of four winter Slavonian garlic cultivars, as well as establishment of correlations between these parameters. The total antioxidant activity and the content of ascorbic acid and total phenols in four cultivars of winter Slavonian garlic were spectrophotometrically determined. Genetic diversity of winter Slavonian garlic cultivars significantly affected the content of ascorbic acid and the total antioxidant activity. The total antioxidant activity significantly positively correlated with the content of ascorbic acid (r2 = 0.64, p = 0.006, while the content of total phenols and total antioxidant activity were not significantly related. Cultivar PFO 1 had the highest antioxidant activity (8.006 μmol Trolox /g FW and the highest ascorbic acid content (8.57 mg /100 g FW, thus, the best quality of the four tested garlic cultivars.

  16. Investigaciones actuales del empleo de Allium sativum en medicina

    Directory of Open Access Journals (Sweden)

    Eslhey María Sánchez Dominguez

    2016-02-01

    Full Text Available Desde tiempos ancestrales el ajo Allium sativum ha sido utilizado por sus propiedades medicinales, ampliamente conocidas. Posee múltiples efectos beneficiosos; tales como: antimicrobiano, hipolipidémico, antitrombótico, actividad antitumoral, antihipertensivo, entre otras. Los compuestos sulfurados presentes en el mismo, principalmente alicina y ajoene, constituyen los principios activos responsables de las actividades biológicas referidas. Se realizó una revisión bibliográfica empleando los recursos disponibles en la red Infomed, específicamente Ebsco, PubMed, Hinari y SciELO, a través de los cuales se accedieron a las bases de datos: Medline, Academic Search Premier, Cochrane Database of Systematic Reviews y MedicLatina, para revisar el estado actual de las investigaciones del ajo en medicina. Existen evidencias científicas que avalan su uso, comprobando los efectos antes referidos. En los últimos años predominan artículos que se centran en el estudio de diferentes formulaciones del ajo: extracto añejo, extracto acuoso, aceite, ajo crudo. En algunas de las referencias consultadas se reconocen limitaciones metodológicas en estas investigaciones. Es consenso que las diferentes formulaciones elaboradas a partir el ajo deben utilizarse como tratamiento complementario.

  17. Processing Pisum sativum seed storage protein precursors in vitro

    Institute of Scientific and Technical Information of China (English)

    YANGLIJUN; CDOMONEY; 等

    1990-01-01

    The profile of polypeptides separated by SDS-PAGE from seed of major crop species such as pea(Pisum sativum) is complex,resulting from cleavage (processing) of precursors expressed from multiple copies of genes encoding vicilin and legumin,the major storage globulins.Translation in vitro of mRNAs hybridselected from mid-maturation pea seed RNAs by defined vicilin and legumin cDNA clones provided precursor molecules that were cleaved in vitro by a cell-free protease extract obtained from similar stage seed;the derived polypeptides were of comparable sizes to those observed in vivo.The feasibility of transcribing mRNA in vitro from a cDNA clone and cleavage in vitro of the derived translation products was established for a legumin clone,providing a method for determining polypeptide products of an expressed sequence.This approach will also be useful for characterising cleavage site requirements since modifications an readily be introduced at the DNA level.

  18. Burdock fructooligosaccharide induces stomatal closure in Pisum sativum.

    Science.gov (United States)

    Guo, Yanling; Guo, Moran; Zhao, Wenlu; Chen, Kaoshan; Zhang, Pengying

    2013-09-12

    Burdock fructooligosaccharide (BFO) isolated from the root tissue of Arctium lappa is a reserve carbohydrate that can induce resistance against a number of plant diseases. Stomatal closure is a part of plant innate immune response to restrict bacterial invasion. In this study, the effects of BFO on stomata movement in Pisum sativum and the possible mechanisms were studied with abscisic acid (ABA) as a positive control. The results showed that BFO could induce stomatal closure accompanied by ROS and NO production, as is the case with ABA. BFO-induced stomatal closure was inhibited by pre-treatment with L-NAME (N(G)-nitro-L-arginine methyl ester, hydrochloride; nitric oxide synthase inhibitor) and catalase (hydrogen peroxide scavenger). Exogenous catalase completely restricted BFO-induced production of ROS and NO in guard cells. In contrast, L-NAME prevented the rise in NO levels but only partially restricted the ROS production. These results indicate that BFO-induced stomatal closure is mediated by ROS and ROS-dependent NO production.

  19. Linhagens de coentro com pendoamento tardio sob dois sistemas de plantio Coriander lines with later-bolting resistance under two planting systems

    Directory of Open Access Journals (Sweden)

    Gabriel M Maciel

    2012-12-01

    comparados com a cv. Verdão.The agronomic performance was evaluated on coriander lines tolerant to early bolting under two production systems (no-tillage and seedling transplant. The experiment was carried out from June to November 2010. Three coriander lines were evaluated (Guarani#1, Guarani#21 and Guarani#36, and one control (cv. Verdão in a randomized complete block experimental design, with three replications. The number of leaves per plant (NF, green mass (MVM, green mass/m² (REND, supported marketable period in the field (COM, cycle (CIC and percentage of bolting (PP, were evaluated. The PP was evaluated in the two production systems at 20, 40, 60, 80, 100 and 120 days after sowing (DAS. Significant differences were obtained in the production system with seedling transplant for NF, MVM and DP between the lines Guarani#36 and Guarani#21 and 'Verdão'. The coriander line Guarani#36 compared to the cv. Verdão was superior in NF, MVM and REND in 246.05%, 162.90% and 165.38%, respectively. Similar superiority was observed in the coriander line Guarani#21 with 211.95%, 148.10% and 150.00% for parameters NF, MVM and REND, respectively. There were no significant differences for CIC between the lines and 'Verdão'. 'In the system of no-tillage the coriander lines Guarani#36 and Guarani#21 presented significant effect in all the evaluated parameters when compared to cv. Verdão, except for cycle of the coriander lines. In the parameters NF, MVM and REND the Guarani#36 coriander line presented superiority of 251.27%, 148.14% and 150% to the cv. Verdão, respectively. The Guarani#21 coriander line compared to cv. Verdão presented superiority of 218.13%, 136.17% and 137.50% for NF, MVM and REND, respectively. The three lines were superior to cv. Verdão on COM for the two tillage systems. The coriander lines Guarani#21 and Guarani#36 present later-bolting in both the systems of production when compared to the cv. Verdão.

  20. Analysis of Volatile Chemical Components of Coriandrum Sativum L. Seed by Gas Chromatography-Mass Spectrometry%气相色谱-质谱法分析芫荽籽挥发油化学成分

    Institute of Scientific and Technical Information of China (English)

    李锋; 解成喜; 范维刚; 符继红

    2005-01-01

    采用水蒸气蒸馏法从新疆产芫荽籽中提取挥发油,收率w=0.78%.用HP-5色谱柱(30 m×0.32 mm×0.25 μm),氢火焰离子化检测器(FID),选取最佳实验条件,用峰面积归一化法测定其相对含量,并用气相色谱-质谱法(GC/MS)对化学成分进行鉴定.共分离出27个峰,鉴定了21个成分, 相对含量占挥发油的99.84%.主要成分为脂肪烃、单萜以及它们的含氧衍生物,其中含量较高的有芳樟醇、内-龙脑、α-葑烯、1-甲基-5-异丙烯基-环己烯和(Z)-2,7-二甲基-5-炔-3-辛烯.本方法稳定可靠.

  1. Determination of Rutin Content in Coriandrum Sativum L.by RP-HPLC%反相高效液相色谱法测定香菜中芦丁的含量

    Institute of Scientific and Technical Information of China (English)

    陈志红; 龚先玲; 徐美奕

    2009-01-01

    本文建立了香菜中芦丁含量的反相高效液相色谱法的测定方法,并测定了不同采收期芦丁的含量.优选测定香菜中芦丁含量的色谱条件为:色谱柱为ODSC18分析柱(4.6mm×250mm,5μm),流动相为甲醇-0.4%的磷酸水溶液(55:45);DAD检测器,检测波长360nm,流速1ml/min,柱温为25℃.实验结果表明,香菜中芦丁含量测定的线性回归方程为y=1064.3349x+18.1259,r=0.9990,线性范围为0.05~0.2μg.香菜样品中芦丁平均含量为4.2551mg/g,平均回收率为96.40%(RSD=1.34%).结果显示,本实验方法稳定、可靠,重复性好,提示可以作为香菜中芦丁含量的测定方法;不同采收期的香菜中芦丁含量略有差别.

  2. 反季节芫荽种子在不同条件下的萌发状态研究%Germination States Research of Counter-season Coriandrum sativum Seed Under Dissimilar Condition

    Institute of Scientific and Technical Information of China (English)

    黄雅琴; 黄群策; 赵帅鹏; 肖琳

    2008-01-01

    为了寻找芜荽反季节栽培的最佳条件,将芜荽种子经过碾压处理、紫外线处理后繁鹩?00mg/L赤霉素、250mg/L芸苔素、100mg/L赤霉素+250mg/L芸苔素混合液浸种处理,观察种子的发芽状态并统计发芽数,计算发芽率等指标.结果表明:经过多种方法处理后能够有效地促进反季节芫荽种子的发芽,其中碾压处理后再以250 mg/L芸苔素浸种,不论发芽率、发芽势,还是活力指数都明显高于其它处理.该项研究为芜荽的反季节栽培提供了参考依据.

  3. 超声波协同双水相体系提取芫荽总黄酮的研究%Aqueous two-phase system and ultrasonic wave co-extraction of total flavones from coriandrum sativum

    Institute of Scientific and Technical Information of China (English)

    王顺民; 季长路; 任晶晶

    2009-01-01

    主要研究了将超声波技术和双水相萃取技术耦合用于提取芫荽中总黄酮.通过单因素实验和正交试验得出,影响芫荽总黄酮的提取因素的主次顺序为溶剂浓度>超声时间>料液比.最佳提取工艺条件为乙醇体积分数50%、料液比1∶20、超声提取20min,总黄酮得率达2.8%.

  4. 重金属胁迫下芫荽生理及挥发性成分变化分析%Analysis of Changes of Physiological Indices and Volatile Constituents of Coriandrum sativum under Heavy Metal Conditions

    Institute of Scientific and Technical Information of China (English)

    魏金凤; 李光勇; 王俊霞; 康文艺

    2013-01-01

    采用不同稀释倍数的废旧电池浸出液胁迫芫荽幼苗,测定叶片生理指标的变化,并用顶空固相微萃取与气质联用技术对芫荽叶中挥发性成分进行分析,研究废旧电池浸出液中重金属对芫荽幼苗生长及挥发性成分的影响.芫荽幼苗对废电池浸出液具有一定的抗性,高质量分数废电池浸出液对芫荽的生长有一定的抑制作用;不同稀释倍数的废电池浸出液对芫荽主要挥发性成分影响较大.结果显示废旧电池在水中渗出的化学物质对芫荽产生较大影响,降低芫荽的食用和药用价值.

  5. Impacts of different germination beds on germinating percentage of Coriandrum sativum L. Seeds%不同发芽床对芫荽种子发芽率的影响

    Institute of Scientific and Technical Information of China (English)

    柳静萍

    2003-01-01

    @@ 芫荽是我省主要的秋冬蔬菜作物.芫荽种子外包果皮,播种到出苗时间相对较长,常常因不良天气等原因造成种子出苗率偏低,为此在种子经营者与购买者之间易发生纠纷.

  6. Extraction and research of properties of the antibacterial components in coriandrum sativum%芫荽抑菌成分的提取及其抑菌性能的研究

    Institute of Scientific and Technical Information of China (English)

    李兴鸣; 徐学明

    2006-01-01

    分别用石油醚、95%乙醇、水浸提芫荽,获得浸提液.抑菌实验表明,水提液对不同细菌均有明显的抑制作用,其最低抑菌浓度都为10%原液.采用沉淀蛋白、分离多糖、超滤、纳滤、离子交换等方法分离水提液组分,证明芫荽中主要抑菌成分是相对分子质量在500~3000的水溶性非肽类物质.

  7. 香菜地上部分挥发活性成分研究%Study on Determination of Chemical Components in Volatile Oil from Coriandrum sativum Leaves and Stem

    Institute of Scientific and Technical Information of China (English)

    刘信平; 张驰; 谭志伟; 于爱农

    2008-01-01

    本实验用水蒸汽蒸馏法提取香菜挥发油,并用气相色谱.质谱联用仪对香菜挥发性物质的化学成分进行了分离和鉴定,分离并鉴定出79个组分,占峰面积的97.63%,并用峰面积归-化法测定了各成分的质量分数.79种化合物中含有醇、酮、醛、酯、酸、烃类化合物及含N、S、Cl、I、Br、P等元素的化合物,其中主要是醇、醛、酮类化合物.其主要挥发性成分为:环己酮、2-十三烯醛、月桂醛、芜荽醇、薄荷呋、1,5.二苯基-3-(2-乙苯)-2-戊烯、肉豆蔻醛、肉珊瑚甙元酮、3-苯基-2-丁醇、2-十二烯醛醇、(Z)乙酸叶醇酯、羊腊醛、2-十二烯醛醇、肉桂-á-苯丙酸甲酯、二环(2.2.1)庚-5-烯-2-基-1.1-二苯-2-戊炔-1.4-二醇、棕榈酸、(Z)-甲酸叶醇酯、乙苯、乳酸顺-3-己烯酯、苯并扁桃腈、甲酸-3-己烯酯.

  8. Allium sativum aqueous extract prevents potassium dichromate-induced nephrotoxicity and lipid oxidation in rats

    Directory of Open Access Journals (Sweden)

    Sergio L. Becerra-Torres

    2014-04-01

    Full Text Available Context: The potassium dichromate (K2Cr2O7 induces nephrotoxicity by oxidative stress mechanisms. Aims: To study the potential protection of an aqueous extract of Allium sativum against the K2Cr2O7-induced nephrotoxicity and lipid oxidation in rats. Methods: Twenty four hours after treatment, biomarkers such as proteinuria, creatinine clearance, malondialdehyde production, specific enzyme activity of gamma glutamyl transpeptidase and alanine aminopeptidase, and renal clearance of para-aminohippuric acid and inulin were measured. Results: The K2Cr2O7 caused significant renal dysfunction, but A. sativum extract prevented this condition by improving all measured biomarkers. Conclusions: A single injection of K2Cr2O7 induced nephrotoxicity in rats, but the supply of an Allium sativum aqueous extract prevented the disorders caused by this metal.

  9. Uji Efek Repellent Nabati Ekstrak Bawang Putih (Allium sativum L.) Terhadap Tikus Putih Jantan Galur Wistar

    OpenAIRE

    Hutagaol, Yanti

    2016-01-01

    Repellent is a substance used as a repellent or insect or other pest deterrent. Repellent is one part of the pesticide.Pesticides are chemical substances used to kill or control pests. In fact, the use of chemical pesticides that are not rational cause negative effects in terms of the environment, especially in terms of human health. Garlic (Allium sativum L.) is a natural substance that has many benefits and rewards. Bulb of garlic (Allium sativum L.) contain substances that are toxic to ins...

  10. In Vitro Antioxidant Properties of Three Varieties of Allium sativum L. Extracts

    Directory of Open Access Journals (Sweden)

    R. T. Narendhirakannan

    2010-01-01

    Full Text Available Many herbs possess antioxidant ingredients that provide efficacy by additive or synergistic activities. Allium sativum L. is a strong astringent, used for the treatment of liver and spleen diseases, rheumatism and tumors. The antioxidant activities of different concentrations of ethanolic extracts of garlic bulb of three varieties were determined by the four assays i.e. DPPH radical scavenging assay, reducing power ability, hydrogen peroxide scavenging assay and total antioxidant capacity. Due to its natural origin and potent free-radical scavenging ability, Allium sativum L. could be used as a potential preventive intervention for free radical-mediated diseases.

  11. Producción y beneficio de semilla de cilantro Production and Post-harvest Handling of Coriander Seed

    Directory of Open Access Journals (Sweden)

    Bernardo Enrique Puga Santos

    2008-10-01

    Full Text Available Se evaluó el potencial de rendimiento de semilla del cultivar Unapal - Precoso utilizando cuatro densidades de siembra, dos métodos de cosecha, tres sistemas de trilla y tres operaciones de limpieza y clasificación. Se usó un diseño de bloques completos al azar con arreglo de parcelas subdivididas con tres repeticiones. La densidad de 1.5 millones ha-1 presentó el mayor rendimiento de semilla pura (1747.2 kg ha-1, aunque no se encontraron diferencias estadísticamente significativas. La cosecha manual rindió 1.264.9kg ha-1 y requirió 19.83 horas-hombre ha-1; la mecánica rindió 1.059kg ha-1 con 5.1 horas-hombre ha-1. El sistema de trilla estacionaria rindió 44.42 kg hora-1, seguido de trilla con garrote (36.97 kg h-1 y trilla con marimba (11.41 kg h-1. La trilla con garrote produjo el material más limpio y con menos fisuras; el porcentaje de frutos partidos fue similar en los tres sistemas de trilla. La venteadora produjo la menor pérdida de semilla (92.3% de recuperación y el mayor índice de remoción de material inerte (78.1%. Las densidades, método de cosecha, trilla y beneficio no afectaron la calidad física y fisiológica de las semillas. La germinación promedio fue de 92% y el índice de velocidad de emergencia (I. V. E. de 8.0 plántulas día-1. La humedad fue de 11.9%.The seed yield potential of coriander cultivar Unapal-Precoso was evaluated using four plant populations, two harvest methods, three threshing systems, and three clean-up and classification processes. A randomized complete block design was used with split-split plots and three replicates. The highest pure seed yield (1747.2 kg ha-1 was obtained with a population of 1.5 million plants/ha, although no statistically significant differences were found among treatments. Manual harvest of seed yielded 1264.9 kg ha-1 and required 19.83 man-hours ha-1, whereas mechanical harvest yielded 1059 kg ha-1 and required 5.1 man-hours ha-1. Stationary threshing yielded 44

  12. A New Coriander Variety——'Hayanyouye'%香菜新品种哈研油叶的选育

    Institute of Scientific and Technical Information of China (English)

    李烨; 于清涛; 刘雨娜; 毕国志; 樊绍翥; 李景富

    2011-01-01

    哈研油叶是从美国油叶香菜品种美国大油叶中分离而来,利用系统育种的方法 2001~2005年共经历5个世代选育而成的香菜新品种.早熟,高产,耐热、耐寒性好,株高25~30 cm,开展度20 cm,叶色青绿,叶片肥大,叶面平滑油亮,叶柄青绿色有光泽,梗粗,根壮,纤维少,香味浓,适应性好,正常条件下出苗后35 d(天)左右即可采收,耐运输,商品性好,适宜我国北方地区种植.%‘ Hayanyouye’is separated from an America coriander variety-‘ Meiguodayouye’ . It was developed by system breeding method. It has gone through 5 generations of selectively breeding from year 2001-2005. This new breed has the following advantages: early maturity, high production, heat-resistant, tolerant to cold. The plant height is 25-30 cm, development is 20 cm. Its leaf is large with dark green color. The leaf blade is smooth with glossy surfaces. It has luster blue-green petiole, and thick stem. The root is strong with few textile fiber and strong fragrance. It has good compatibility. Under normal condition it takes about 35 days from seedling to harvesting. It is tolerant to transportation and has good commercial value. It is suitable for cultivation in North of China.

  13. Pea (Pisum sativum L. in the Genomic Era

    Directory of Open Access Journals (Sweden)

    Robert J. Redden

    2012-04-01

    Full Text Available Pea (Pisum sativum L. was the original model organism used in Mendel’s discovery (1866 of the laws of inheritance, making it the foundation of modern plant genetics. However, subsequent progress in pea genomics has lagged behind many other plant species. Although the size and repetitive nature of the pea genome has so far restricted its sequencing, comprehensive genomic and post genomic resources already exist. These include BAC libraries, several types of molecular marker sets, both transcriptome and proteome datasets and mutant populations for reverse genetics. The availability of the full genome sequences of three legume species has offered significant opportunities for genome wide comparison revealing synteny and co-linearity to pea. A combination of a candidate gene and colinearity approach has successfully led to the identification of genes underlying agronomically important traits including virus resistances and plant architecture. Some of this knowledge has already been applied to marker assisted selection (MAS programs, increasing precision and shortening the breeding cycle. Yet, complete translation of marker discovery to pea breeding is still to be achieved. Molecular analysis of pea collections has shown that although substantial variation is present within the cultivated genepool, wild material offers the possibility to incorporate novel traits that may have been inadvertently eliminated. Association mapping analysis of diverse pea germplasm promises to identify genetic variation related to desirable agronomic traits, which are historically difficult to breed for in a traditional manner. The availability of high throughput ‘omics’ methodologies offers great promise for the development of novel, highly accurate selective breeding tools for improved pea genotypes that are sustainable under current and future climates and farming systems.

  14. Physiological toxic effects of leachate on coriander%垃圾渗滤液对香菜的生理毒害作用

    Institute of Scientific and Technical Information of China (English)

    易秀; 陈生婧; 徐景景; 魏茅

    2012-01-01

    【目的】研究不同体积分数垃圾渗滤液对香菜生长的影响,旨在为探明垃圾渗滤液对植物的毒害机理,从而为渗滤液的达标排放提供科学依据。【方法】以新西兰大叶香菜为研究对象,采用盆栽试验和培养皿法,研究不同体积分数(0%(对照),50%,67%,75%)垃圾渗滤液对香菜叶片叶绿素含量、外渗电导率、蛋白质含量及种子发芽率和发芽势的影响。【结果】1)随着垃圾渗滤液体积分数的增大,香菜叶片中叶绿素含量逐渐减少,当渗滤液体积分数为75%时,香菜叶片叶绿素含量降至最小值9.21mg/g;香菜叶片叶绿素a/b值总体也呈减小的趋势。2)随着渗滤液体积分数的增大,香菜叶片外渗电导率呈正比例增加,当渗滤液体积分数为75%时,叶片外渗电导率达到最大值512.0mS/cm。3)当渗滤液体积分数从0%增加到67%时,香菜叶片中蛋白质含量降至最小值8.26g/kg;之后随着渗滤液体积分数的增加,香菜叶片蛋白质含量呈增大的趋势。4)随着渗滤液体积分数的增加,香菜种子发芽势和发芽率均呈先增加后减小的趋势。【结论】不同体积分数垃圾渗滤液均可导致香菜叶片光合作用减弱;当体积分数高于67%时,渗滤液会毒害香菜种子,使其发芽率和发芽势降低。%【Objective】 The research was made in order to understand toxic effects of leachate on growth of coriander to probe into toxicant mechanisms of leachate on plants,and to provide scientific basis for discharge standards of leachate.【Method】 Some pot experiments and Petri dishes method have been processed,and effects of different volumes of leachate(0%(CK),50%,67%,75%) on chlorophyll contents,exosmose conductivity rate,protein contents,germination rates and germination potential have been studied by taking coriander as a research object.【Result】 The results show that the chlorophyll contents of coriander leaf decrease

  15. A national outbreak of verotoxin-producing Escherichia coli O157 associated with consumption of lemon-and-coriander chicken wraps from a supermarket chain.

    Science.gov (United States)

    Whittaker, P J; Sopwith, W; Quigley, C; Gillespie, I; Willshaw, G A; Lycett, C; Surman-Lee, S; Baxter, D; Adak, G K; Syed, Q

    2009-03-01

    A national outbreak of verotoxin-producing Escherichia coli O157 infection affected five English regions and Wales. Twelve cases were associated with lemon-and-coriander chicken wrap from a single supermarket chain consumed over a 5-day period. An outbreak investigation aimed to identify the source of infection. Descriptive epidemiology and phenotypic and genotypic tests on human isolates indicated a point-source outbreak; a case-control study showed a very strong association between consumption of lemon-and-coriander chicken wrap from the single supermarket chain and being a case (OR 46.40, 95% CI 5.39-infinity, P=0.0002). Testing of raw ingredients, products and faecal samples from staff in the food production unit did not yield any positive results. The outbreak was probably caused by one contaminated batch of an ingredient in the chicken wrap. Even when current best practice is in place, ready-to-eat foods can still be a risk for widespread infection.

  16. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies) , 2013. Scientific Opinion on the safety of “ coriander seed oil ” as a Novel Food ingredient

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on “coriander seed oil (CSO)” as a novel food ingredient (NFI) in the context of Regulation (EC) No 258/97. Petroselinic acid (PA) is the ......Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on “coriander seed oil (CSO)” as a novel food ingredient (NFI) in the context of Regulation (EC) No 258/97. Petroselinic acid (PA......) is the major fatty acid in CSO. Conventional edible oil technologies are used to manufacture the NFI. The NFI is intended to be marketed as a food supplement for healthy adults, at a maximum level of 600 mg per day (i.e. 8.6 mg/kg bw per day for a 70 kg person), which would lead to significantly higher intakes...

  17. Effects of dietary supplementation of coriander oil, in canola oil diets, on the metabolism of [1-(14)C] 18:3n-3 and [1-(14)C] 18:2n-6 in rainbow trout hepatocytes.

    Science.gov (United States)

    Randall, K M; Drew, M D; Øverland, M; Østbye, T-K; Bjerke, M; Vogt, G; Ruyter, B

    2013-09-01

    The aim of this study was to investigate the effects of petroselinic acid, found in coriander oil, on the ability of rainbow trout hepatocytes to increase the production of eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) from [1-(14)C] α-linolenic acid (18:3n-3; ALA) and to reduce the production of arachidonic acid (20:4n-6; ARA) from [1-(14)C] 18:2n-6. Addition of coriander oil increased the production of 22:6n-3, from [1-(14)C] 18:3n-3, at the 0.5 and 1.0% inclusion levels and reduced the conversion of [1-(14)C] 18:2n-6 to 20:4n-6. β-Oxidation was significantly increased at the 1.5% inclusion level for [1-(14)C] 18:2n-6, however β-oxidation for [1-(14)C] 18:3n-3 only showed an increasing trend. Acetate, a main breakdown product of fatty acids (FA) via peroxisomal β-oxidation, decreased three-fold for [1-(14)C] 18:2n-6 and nearly doubled for [1-(14)C] 18:3n-3 when coriander was added at a 1.5% inclusion level. Acyl coenzyme A oxidase (ACO) enzyme activity showed no significant differences between treatments. Relative gene expression of ∆6 desaturase decreased with addition of coriander oil compared to the control. The addition of petroselinic acid via coriander oil to vegetable oil (VO) based diets containing no fishmeal (FM) or fish oil (FO), significantly increased the production of anti-inflammatory precursor 22:6n-3 (P=0.011) and decreased pro-inflammatory precursor 20:4n-6 (P=0.023) in radiolabelled hepatocytes of rainbow trout.

  18. Genetic Diversity of Chinese and Global Pea (Pisum sativum L.) Collections.

    Science.gov (United States)

    Pea (Pisum sativum L.) is an important food and feed legume grown across many temperate regions of the world, especially from Asia to Europe and North America. The goal of this study was to use 30 informative pea microsatellite markers to compare genetic diversity in a global core from the USDA and ...

  19. Antioxidative response of Lepidium sativum L. during assisted phytoremediation of Hg contaminated soil.

    Science.gov (United States)

    Smolinska, Beata; Szczodrowska, Agnieszka

    2017-09-25

    In this study, Lepidium sativum L. was used in repeated phytoextraction processes to remove Hg from contaminated soil, assisted by combined use of compost and iodide (KI). L. sativum L. is sensitive to changes in environmental conditions and has been used in environmental tests. Its short vegetation period and ability to accumulate heavy metals make it suitable for use in repeated phytoextraction. The antioxidant enzymatic system of the plant (catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and glutathione S-transferase (GST)) was analysed, to understand the effects of increasing Hg accumulation and translocation. Phytoextraction was repeated six times to decrease Hg contamination in soil, and the efficiency of each step was assessed. The results indicate that L. sativum L. is able to take up and accumulate Hg from contaminated soil. A corresponding increase in enzymatic antioxidants shows that the plant defence system is activated in response to Hg stress. Using compost and KI increases total Hg accumulation and translocation to the above-ground parts of L. sativum L. Repeating the process decreases Hg contamination in pot experiments in all variants of the process. The combined use of compost and KI during repeated phytoextraction increases the efficiency of Hg removal from contaminated soil. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. Danish Rhizobium leguminosarum strains nodulating ‘Afghanistan’ pea (Pisum sativum)

    DEFF Research Database (Denmark)

    Jensen, Erik Steen; Sørensen, Lasse Holst; Engvild, Kjeld Christensen

    1986-01-01

    A wild pea (Pisum sativum L.) native to Afghanistan normally known to be resisant to nodulation with European strains of Rhizobium leguminosarum was nodulated early and effectively in field soil in Denmark. Isolates from nodules formed effective nodules abundantly on 'Afghanistan' on reinfection...... pattern with Rhizobium leguminosarum strains isolated from a modern pea variety cultivated in the same field....

  1. The role of phosphorus in nitrogen fixation by young pea plants (Pisum sativum)

    DEFF Research Database (Denmark)

    Jakobsen, Iver

    1985-01-01

    The influence of P on N2 fixation and dry matter production of young pea (P. sativum L. cv. Bodil) plants grown in a soil-sand mixture was investigated in growth cabinet experiments. Nodule dry weight, specific C2H2 reduction and P concentration in shoots responded to P addition before any growth...

  2. Pea (Pisum sativum) Seed Production as an Assay for Reproductive Effects Due to Herbicides.

    Science.gov (United States)

    Even though herbicide drift can affect plant reproduction, current plant testing protocols emphasize effects on vegetative growth. In this study, we determined whether a short–growing season plant can indicate potential effects of herbicides on seed production. Pea (Pisum sativum...

  3. Protective Effects of Quercetin against Dimethoate-Induced Cytotoxicity and Genotoxicity in Allium sativum Test.

    Science.gov (United States)

    Ahmad, Waseem; Shaikh, Sibhghatulla; Nazam, Nazia; Lone, Mohammad Iqbal

    2014-01-01

    The present investigation was directed to study the possible protective activity of quercetin-a natural antioxidant against dimethoate-induced cyto- and genotoxicity in meristematic cells of Allium sativum. So far there is no report on the biological properties of quercetin in plant test systems. Chromosome breaks, multipolar anaphase, stick chromosome, and mitotic activity were undertaken in the current study as markers of cyto- and genotoxicity. Untreated control, quercetin controls (@ 5, 10 and 20 μg/mL for 3 h), and dimethoate exposed groups (@ 100 and 200 μg/mL for 3 h) were maintained. For protection against cytogenotoxicity, the root tip cells treated with dimethoate at 100 and 200 μg/mL for 3 h and quercetin treatment at 5, 10, and 20 μg/mL for 16 h, prior to dimethoate treatment, were undertaken. Quercetin was found to be neither cytotoxic nor genotoxic in Allium sativum control at these doses. A significant increase (P < 0.05) in chromosomal aberrations was noted in dimethoate treated Allium. Pretreatment of Allium sativum with quercetin significantly (P < 0.05) reduced dimethoate-induced genotoxicity and cytotoxicity in meristematic cells, and these effects were dose dependent. In conclusion, quercetin has a protective role in the abatement of dimethoate-induced cyto- and genotoxicity in the meristematic cells of Allium sativum that resides, at least in part, on its antioxidant effects.

  4. First Report of Garlic Rust Caused by Puccinia allii on Allium sativum in Minnesota

    Science.gov (United States)

    In July 2010, Allium sativum, cultivar German Extra Hardy Porcelain plants showing foliar symptoms typical of rust infection were brought to the Plant Disease Clinic at the University of Minnesota by a commercial grower from Fillmore county Minnesota. Infected leaves showed circular to oblong lesio...

  5. Histological Evaluation of Allium sativum Oil as a New Medicament for Pulp Treatment of Permanent Teeth.

    Science.gov (United States)

    Mohammad, Shukry Gamal; Raheel, Syed Ahmed; Baroudi, Kusai

    2015-02-01

    The objective of this study was to evaluate the histo pathology effects of two medicaments Allium sativum oil and formocresol on the remaining pulp tissue of the permanent teething children. A total of 18 premolars were included in this study. Two sound premolars were extracted and subjected to histological examination to show the normal pulp tissue. Pulpo tomy procedure was performed in the rest of the remaining 16 premolars; half of them using Allium sativum oil and the rest of the tested premolars were medicated using formocresol and all were sealed with suitable restoration. Then, premolars extracted at variable intervals (48 hours, 2 weeks, 1 month, 2 months), stained using hemotoxylin and eosin etain (H&E) and prepared for histopathology examination. Histological evaluation seemed far more promising for Allium sativum oil than formocresol. Histological evaluation revealed that teeth treated with Allium sativa oil showed infammatory changes that had been resolved in the end of the study. On the contrary, the severe chronic infammation of pulp tissue accompanied with formocresol eventually produced pulp necrosis with or without fibrosis. In addition, pulp calcification was evidenced in certain cases. Allium sativum oil is a biocompatible material that is compatible with vital human pulp tissue. It offers a good healing potential, leaving the remaining pulp tissue healthy and functioning.

  6. A Comparison between the Antioxidant Strength of the Fresh and Stale Allium sativum (Garlic Extracts

    Directory of Open Access Journals (Sweden)

    Fatemeh Taji

    2012-05-01

    Full Text Available Background: Fruits and vegetables are considered as the source of antioxidant and the factor of destroying reactive oxygen species. The effect of this antioxidant might decrease in time. This study was aimed at examining and comparing the antioxidant effect and the level of phenolic and flavonoid compounds as well as allicin level, between fresh and three-month stale Allium sativum (garlic.Materials and Methods: In an experimental study, the ethanolic extract of bushes of fresh and three-month-old Allium sativum was prepared and their antioxidant activities were assessed in linoleic acid and β-carotene linoleate system. The amount of phenolic compounds was measured by Folin-Ciocalteumethod, based on gallic acid; the amount of flavonol and flavonoid compounds by aluminum chloride base on rutin base; and the amount of allicinby spectrophotometry method. SPSS-15 Software and t-statistic tests were used to analyze the mean difference between the results of two groups and p<0.05 was considered as significant. Results: The efficiency of fresh garlic (35.36 in inhibiting the oxidation was higher (p<0.05 comparing to three-month dated Allium sativum (10.2. Phenolic compounds of the fresh garlic (12.61mg/g were more than the three-month dated Allium sativum (2.89mg/g. The amount of allicin was respectively 15 µg/ml and 8 µg/ml in the fresh and three-month dated Allium sativum (p<0.05.Conclusion: The fresh garlic contains more useful substances and it is recommended to be used in its fresh estate.

  7. Antioxidative activity of different parts of the plant Lepidium sativum Linn

    Directory of Open Access Journals (Sweden)

    Jency Malar

    2014-09-01

    Full Text Available Lepidium sativum Linn. has been used in traditional and folklore medicine for the treatment of bronchial asthma, diabetes, local and rheumatic pain. An ethanolic extract of cress (L. sativum L. shoot, leaf, stem and seed has been studied for antioxidative active against 1,1-diphenyl-2-picrylhydrazyl (DPPH, total glutathione S-transferase assay, reduced glutathione activity, reducing power (Fe3+–Fe2+ Transformation Ability, and ascorbic acid is also estimated. The percentage yields of free radical scavenging activity (DPPH obtained for different ethanolic extracts of L. sativum. Supreme scavenging activity was detected in shoot (12.19 ± 02% and least in stem (2.69 ± 05%. The activity of total glutathione S-transferase enzyme was found to be more in seed (9600 ± 56.3 μg/ml than other plant parts. The reduced glutathione content of the ethanolic extracts of L. sativum was found to be more in leaf (9 ± 0.2 μg/ml. In the reducing power assay, ethanolic extracts gives the optical density in increasing concentration in all plant parts it shows that it has the reducing ability of Fe3+–Fe2+. Presence of vitamin C was tested. It was found that the shoot extract has highest amount of vitamin C. The results of present data were shown that the ethanolic extract of L. sativum L. plant parts have contributed high potential in vitro antioxidant activity.

  8. Radiation processing of minimally processed vegetables and aromatic plants

    Energy Technology Data Exchange (ETDEWEB)

    Trigo, M.J. [Instituto Nacional dos Recursos Biologicos, L-INIA, Quinta do Marques, 2784-505 Oeiras (Portugal)], E-mail: mjptrigo@gmail.com; Sousa, M.B.; Sapata, M.M.; Ferreira, A.; Curado, T.; Andrada, L. [Instituto Nacional dos Recursos Biologicos, L-INIA, Quinta do Marques, 2784-505 Oeiras (Portugal); Botelho, M.L. [Instituto Tecnologico e Nuclear, E.N. 10, 2696 Sacavem (Portugal); Veloso, M.G. [Faculdade de Medicina Veterinaria de Lisboa, Av. da Universidade Tecnica, Alto da Ajuda, 1300-477 Lisboa (Portugal)

    2009-07-15

    Vegetables are an essential part of people's diet all around the world. Due to cultivate techniques and handling after harvest, these products, may contain high microbial load that can cause food borne outbreaks. The irradiation of minimally processed vegetables is an efficient way to reduce the level of microorganisms and to inhibit parasites, helping a safe global trade. Evaluation of the irradiation's effects was carried out in minimal processed vegetables, as coriander (Coriandrum sativum L.), mint (Mentha spicata L.), parsley (Petroselinum crispum Mill, (A.W. Hill)), lettuce (Lactuca sativa L.) and watercress (Nasturium officinale L.). The inactivation level of natural microbiota and the D{sub 10} values of Escherichia coli O157:H7 and Listeria innocua in these products were determined. The physical-chemical and sensorial characteristics before and after irradiation at a range of 0.5 up to 2.0 kGy applied doses were also evaluated. No differences were verified in the overall of sensorial and physical properties after irradiation up to 1 kGy, a decrease of natural microbiota was noticed ({>=}2 log). Based on the determined D{sub 10}, the amount of radiation necessary to kill 10{sup 5}E. coli and L. innocua was between 0.70 and 1.55 kGy. Shelf life of irradiated coriander, mint and lettuce at 0.5 kGy increased 2, 3 and 4 days, respectively, when compared with non-irradiated.

  9. Musca domestica laboratory susceptibility to three ethnobotanical culinary plants.

    Science.gov (United States)

    El Zayyat, Elham A; Soliman, Mohammed I; Elleboudy, Noha A; Ofaa, Shaimaa E

    2015-10-01

    Throughout history, synanthropic Musca domestica had remained a worldwide problem whenever poor sanitation and bad hygienic conditions exists. Houseflies growing resistance to chemical insecticides are a rising environmental problem that necessitates search for alternatives. Mentha cervina, Ocimum basilicum, and Coriandrum sativum were tested for bioactivity on M. domestica adults and larvae. They are culinary Mediterranean plants. In adulticidal bioassay, using both CDC bottles and fumigation techniques, basil was the most effective extract with LC50 1.074 and 34.996 g/L, respectively. Concerning larvicidal bioassay by fumigation technique, coriander had the highest toxicity index with LC50 29.521 g/L. In both dipping and feeding technique, basil had the highest toxicity with LC50 32.643 and 0.749 g/L, respectively. Basil showed the highest toxicity results in four out of the five models tested followed by coriander then mint; this result highlights the potentiality of basil as a green insecticide in management of flies and opens new insight in the industrialization of basil-based fly control products.

  10. Radiation processing of minimally processed vegetables and aromatic plants

    Science.gov (United States)

    Trigo, M. J.; Sousa, M. B.; Sapata, M. M.; Ferreira, A.; Curado, T.; Andrada, L.; Botelho, M. L.; Veloso, M. G.

    2009-07-01

    Vegetables are an essential part of people's diet all around the world. Due to cultivate techniques and handling after harvest, these products, may contain high microbial load that can cause food borne outbreaks. The irradiation of minimally processed vegetables is an efficient way to reduce the level of microorganisms and to inhibit parasites, helping a safe global trade. Evaluation of the irradiation's effects was carried out in minimal processed vegetables, as coriander ( Coriandrum sativum L .), mint ( Mentha spicata L.), parsley ( Petroselinum crispum Mill, (A.W. Hill)), lettuce ( Lactuca sativa L.) and watercress ( Nasturium officinale L.). The inactivation level of natural microbiota and the D 10 values of Escherichia coli O157:H7 and Listeria innocua in these products were determined. The physical-chemical and sensorial characteristics before and after irradiation at a range of 0.5 up to 2.0 kGy applied doses were also evaluated. No differences were verified in the overall of sensorial and physical properties after irradiation up to 1 kGy, a decrease of natural microbiota was noticed (⩾2 log). Based on the determined D10, the amount of radiation necessary to kill 10 5E. coli and L. innocua was between 0.70 and 1.55 kGy. Shelf life of irradiated coriander, mint and lettuce at 0.5 kGy increased 2, 3 and 4 days, respectively, when compared with non-irradiated.

  11. Clinical and Radiographic Evaluation of Allium sativum Oil as a New Medicament for Vital Pulp Treatment of Primary Teeth

    OpenAIRE

    Mohammad, Shukry Gamal; Raheel, Syed Ahmed; Baroudi, Kusai

    2014-01-01

    Background: The objective of this study was to compare between the clinical and radiographic effects of Allium sativum oil and those of formocresol in vital pulpotomy in primary teeth. Materials and Methods: A total of 20 children age ranged from 4 to 8 years were included in the study. In every one of those children, the primary molars indicated for pulpotomy. Pulpotomy procedure was performed, and the radicular pulp tissue of one molar capped with A. sativum oil in a cotton pellet, whereas ...

  12. Assessment of the potential of Allium sativum oil as a new medicament for non-vital pulpotomy of primary teeth

    OpenAIRE

    Mohammad, Shukry Gamal; Baroudi, Kusai

    2015-01-01

    Objective: The objective of this study was to compare the clinical and radiographic effects of Allium sativum oil and formocresol in nonvital pulpotomy in primary teeth. Materials and Methods: Twenty children ranging in age from 4 to 8 years were included in the study. In every one of those children, pulpotomy was indicated for the primary molars. Pulpotomy procedure was performed and the radicular pulp tissue of one molar was capped with A. sativum oil in a cotton pellet while the other mola...

  13. INFLUENCE OF NOSTOC VAUCHER EX BORNET ET FLAHAULT STRAINS ON GROWTH AND DEVELOPMENT OF PISUM SATIVUM L.

    Directory of Open Access Journals (Sweden)

    Maltsev Ye. I.

    2015-12-01

    Full Text Available We investigated the positive impact of cultures cyanobacteria genus Nostoc Vaucher ex Bornet et Flahault on growth and development of higher plants as an example Pisum sativum L. All the Nostoc species have a positive effect on the viability, germination energy, and biometric characteristics of Pisum sativum. The greatest positive influence was registered for N. entophytum Born. et. Flah. and N. linckia (Roth Bornetet Flahault f. linckia.

  14. INFLUENCE OF NOSTOC VAUCHER EX BORNET ET FLAHAULT STRAINS ON GROWTH AND DEVELOPMENT OF PISUM SATIVUM L.

    Directory of Open Access Journals (Sweden)

    Ye. I. Maltsev

    2015-12-01

    Full Text Available We investigated the positive impact of cultures cyanobacteria genus Nostoc Vaucher ex Bornet et Flahault on growth and development of higher plants as an example Pisum sativum L. All the Nostoc species have a positive effect on the viability, germination energy, and biometric characteristics of Pisum sativum. The greatest positive influence was registered for N. entophytum Born. et. Flah. and N. linckia (Roth Bornetet Flahault f. linckia. Keywords: Nostoc, seed pelleting, viability, germination energy, biomass.

  15. Incorporation of Allium sativum in yogurt: In vitro study on inhibition of diabetes- and hypertension-associated enzymes

    Directory of Open Access Journals (Sweden)

    Shabboo Amirdivani Amirdivani

    2015-06-01

    Full Text Available The effects of inclusion of Allium sativum on yogurt formation and subsequent storage (4°C, up to 28 days on proteolysis, microbial activity, the inhibition of a-amylase, a-glucosidase and angiotensin-1 converting enzyme (ACE-1 in vitro were investigated. A. sativum-yogurt showed higher rates of pH reduction and increment of TA than plain-yogurt during incubation at 41°C. Highest proteolysis,  on day 7 showed in A. sativum-yogurt (62.7±0.80 mg/mL, which was 2-flod higher than plain yogurt (31.0±0.96 mg/mL. Bacterial counts in A.sativum-yogurt were higher for Lactobacillus spp. but lower for S. thermophillus (p<0.05 compared to those in plain yogurt throughout refrigerated storage. Highest inhibitory activities for α-amylase were recorded on day 14 of storage for A. sativum- and plain-yogurts (IC50= 13.7±1.99and 26.3±2.15mg respectively; p<0.05 and on day 7 for α-glucosidase (IC50= 120.7±22.71 and 192.3±33.24mg respectively; p<0.05. The highest anti-ACE-I activity was observed on day 7 of refrigerated storage with A. sativum-yogurt (IC50=6.9±0.23mg being more potent than plain-yogurt (IC50=9.7±0.12mg; p<0.05. A. sativum-yogurt was not favoured for overall aroma, sourness and bitterness in the sensory evaluations but recorded the same overall preference as plain yogurt. A. sativum enhanced the fermentation of yogurt in favour of the population of Lactobacillus spp, stimulated proteolysis of milk proteins and increased the in vitro inhibition of key enzymes associated with diabetes and hypertension.

  16. Garden cress (Lepidium sativum Linn.) seed oil as a potential feedstock for biodiesel production.

    Science.gov (United States)

    Nehdi, Imededdine Arbi; Sbihi, Hassen; Tan, Chin Ping; Al-Resayes, Saud Ibrahim

    2012-12-01

    Lepidium sativum L. (garden cress) is a fast growing annual herb, native to Egypt and west Asia but widely cultivated in temperate climates throughout the world. L. sativum seed oil (LSO) extracted from plants grown in Tunisia was analyzed to determine whether it has potential as a raw material for biodiesel production. The oil content of the seeds was 26.77%, mainly composed of polyunsaturated (42.23%) and monounsaturated (39.62%) fatty acids. Methyl esters (LSOMEs) were prepared by base-catalyzed transesterification with a conversion rate of 96.8%. The kinematic viscosity (1.92 mm(2)/s), cetane number (49.23), gross heat value (40.45), and other fuel properties were within the limits for biodiesel specified by the ASTM (American Standard for Testing and Materials). This study showed that LSOMEs have the potential to supplement petroleum-based diesel.

  17. Regeneration of Pea (’Pisum sativum L’.) Plants from Shoot Apical Meristems,

    Science.gov (United States)

    A procedure has been developed to obtain complete plants from meristems of three cultivars of Pisum sativum L. Benzyladenine (BA) alone or in...combination with naphthaleneacetic acid (NAA) at molar concentrations of 5 x .0000005 and .000001 respectively, induced shoot differentiation in meristems ...complete plant formation. Root formation, on the shoots produced by culturing meristems was induced by reculturing the shoots, 2 cm long, on half strength B5 medium supplemented with NAA at a concentration of .000001 M.

  18. Nitrogen and phosphorus compounds in the aleurone grains of Iris pseudoacorus endosperm and Pisum sativum cotyledons

    OpenAIRE

    Ligia Konopska

    2015-01-01

    Aleurone grains from Iris pseudoacorus endosperm and Pisum sativum cotyledons were isolated partly according to Tombs's method (1967). Nitrogen compounds content was determined in them by Kjeldahl's micromethod, and in the particular fractions after Thiman and Laloraya (1960). Mainly protein N was detected in the aleurone grains, constituting 14.8 and 15.2 per cent of the dry mass of pea and Iris seeds, respectively. Moreover, phosphorus compounds were fractionated according to Holden and Pir...

  19. 芫荽软腐病病原菌生物学特性与药剂筛选%Biology Characters and Bactericide Screening of Pathogen of Coriander Soft Rot

    Institute of Scientific and Technical Information of China (English)

    张立微; 张景涛; 张丽茁; 李小梅; 戴忠仁

    2015-01-01

    为有效防治芫荽软腐病,对其病原菌进行生物学特性及其药敏筛选试验研究。结果表明:芫荽软腐病病原菌在37℃条件下,土壤p H为7时导致芫荽植株发病加速。在药剂筛选试验中,通过抑菌圈法可知,7种试验药剂中72%农用硫酸链霉素可溶性粉剂、3%噻霉酮可湿性粉剂、53.8%氢氧化铜水分散粒剂、20%噻菌铜悬浮剂均有明显抑菌作用。其中,72%硫酸链霉素可溶性粉剂的抑菌作用最强,最小抑菌浓度为0.031%;其次为3%噻霉酮可湿性粉剂,最小抑菌浓度为0.06%。%For effective prevention and control of coriander soft rot ,biology characters and bactericide screening of pathogen of coriander soft rot were studied .The result showed that coriander soft rot could be accelerated plant disease under 37℃ and pH 7 .0 of soil .According to bactericide screening ,72% Streptomycin SP ,3% Ben‐ziothiazolinone WP ,53 .8% Copper hydroxide WDG ,20% Thiodiazole‐copper SC were effected to coriander soft rot .Minimum inhibitory concentration of 72% Streptomycin SP was 0 .031% ;secondly ,minimum inhibitory concentration of 3% Benziothiazolinone WP was 0 .06% .

  20. PHYTOCHEMICAL SCREENING AND DIURETIC ACTIVITY OF ALLIUM SATIVUM STEROIDAL AND TRITERPENOID SAPONIN FRACTION

    Directory of Open Access Journals (Sweden)

    Dr. Papiya Bigoniya et al

    2012-09-01

    Full Text Available Allium sativum L. (Liliacea is a perennial bulb with a tall, erect flowering stem. The bulb of the plant has been used in many parts of the world as a stimulant, carminative, antiseptic, expectorant, anthelmintic and diuretic. This study has been planned to assess the diuretic activity of fresh garlic bulb extract targeting the steroidal and triterpenoidal saponin content. The rats were randomly divided into 4 groups of 5 animal each as vehicle control (2 % tragacanth suspension, standard drug frusemide (20 mg/kg, p.o, and n-butanol extract (10 mg/kg and 20 mg/kg, p.o treated. Urine was collected in a graduated cylinder and its volume was measured for next 5 hr. Na+, K+ and Cl- concentrations were measured. Phytochemical analysis of A. sativum n-butanol fraction showed presence of steroids, triterpenoidal saponins and carbohydrates. At 20 mg/kg dose onset of diuresis and total volume of urine formed was significantly (P<0.01-0.05 higher. Fifth hour urine volume at 20 mg/kg dose was 9.3 ml as compared to 5.5 ml of control. Extract at 20 mg/kg dose produced 24.57% increase in Na+ excretion against 132.65% increase by frusemide when compared to control signifying natriuretic and aquaretic response. The study confirmed the ethnopharmacological and Ayurvedic use of A. sativum as a diuretic agent.

  1. Genetic diversity analysis of Lepidium sativum (Chandrasur) using inter simple sequence repeat (ISSR) markers

    Institute of Scientific and Technical Information of China (English)

    Amandeep Kaur; Rakesh Kumar; Suman Rani; Anita Grewal

    2015-01-01

    Lepidium sativum (commonly known as garden cress) belongs to the family Brassicaceae. It is a fast-growing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic, hypoglycemic, nephrocurative and nephroprotective activ-ities. In the present study, we assessed the genetic diversity of various genotypes of L. sativum using inter-simple sequence repeat (ISSR) markers. Out of 41 ISSR primers screened, 32 primers showed significant, clear and repro-ducible bands. A total of 510 amplified bands were obtained using 32 ISSR primers, out of which 422 bands were poly-morphic and 88 bands were monomorphic. The percentage of polymorphism was found to be 82. A total of 35 unique alleles ranging insize from 200 to 2,900 bp were observed. Cluster analysis based on unweighted pair-group method, arithmetic mean divided the 18 genotypes into two main clusters, with the first having only HCS-08 genotype of L. sativum and other having all of the other 17 genotypes. The Jaccard similarity coefficient revealed a broad range 32–72%genetic relatedness among the 18 genotypes.

  2. Purification and Properties of Superoxide Dismutase(SOD) in Allium Sativum

    Institute of Scientific and Technical Information of China (English)

    TENG Li-rong; WANG Ya-jun; WU Min; HONG Shui-sheng; CHEN Jia; Meng Qing-fan; LIU Lan-ying

    2003-01-01

    Superoxide dismutases(SODs) were purified to homogeneity from Allium Sativum by means of ammonium sulfate precipitation and column chromatography with DEAE-cellulose(DE52) and Sephadex G-75. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-AGE), Allium Sativum is predicted to contain four SODs. The molecular weights of the native SODs are 41.3 kD, 37.0 kD, 35.2 kD and 31.0 kD, which consist of subunits of 20.7 kD, 18.4 kD, 17.7 kD and 15.4 kD respectively. Because of their specific sensitivity to hydrogen peroxide, cyanogens potassium and chloroform-alcohol, the SODs in Allium Sativum appear to be Cu, Zn-SOD isoenzymes. The isoelectric analysis indicates that three of the four isoenzymes are acidic proteins with isoelectric points at pH 3.5, 3.7 and 4.0, respectively, and the fourth one is a basic protein with isoeletric point at pH 8.5.

  3. GC-MS Analysis of Coriander Volatile Oils from Yuxi County in Yunnan Province%云南玉溪芜荽挥发油成分的GG-MS分析

    Institute of Scientific and Technical Information of China (English)

    张京娜; 陈霞; 杨冬; 张颖君; 杨崇仁

    2009-01-01

    目的 分析云南玉溪产芜荽的挥发油成分,比较不同产地芜荽挥发油组成,讨论其差异.方法 采用水蒸气蒸馏法从云南玉溪产芜荽中提取挥发油,通过气相色谱-质谱联用系统对挥发性成分进行分析.结果 检出41个色谱峰,用峰面积归一化法得出色谱峰的相时含量;与数据库时照,鉴定了其中的35个化合物.结论 云南玉溪产芜荽的挥发油成分与文献报道其它产地的有显著差异.%Objective To discuss the chemical differences of Coriander volatile oils from different producing areas. Methods Coriander volatile oils from Yuxi county of Yunnan was obtained by steam distillation extraction method. Results There are 41 peaks from the volatile oils were showed by GC-MS analysis. The relative content of each peak was detected by the normalization method of peak area. Among them, 35 peaks were identified by compared with MS database. Conclusion The volatile oils components of Yuxi Coriander had significant differences with that of other producing areas.

  4. Antibacterial activity of natural spices on multiple drug resistant Escherichia coli isolated from drinking water, Bangladesh

    Directory of Open Access Journals (Sweden)

    Islam Rezuanul

    2011-03-01

    Full Text Available Abstract Background Spices traditionally have been used as coloring agents, flavoring agents, preservatives, food additives and medicine in Bangladesh. The present work aimed to find out the antimicrobial activity of natural spices on multi-drug resistant Escherichia coli isolates. Methods Anti-bacterial potentials of six crude plant extracts (Allium sativum, Zingiber officinale, Allium cepa, Coriandrum sativum, Piper nigrum and Citrus aurantifolia were tested against five Escherichia coli isolated from potable water sources at kushtia, Bangladesh. Results All the bacterial isolates were susceptible to undiluted lime-juice. None of them were found to be susceptible against the aqueous extracts of garlic, onion, coriander, pepper and ginger alone. However, all the isolates were susceptible when subjected to 1:1:1 aqueous extract of lime, garlic and ginger. The highest inhibition zone was observed with lime (11 mm. Conclusion Natural spices might have anti-bacterial activity against enteric pathogens and could be used for prevention of diarrheal diseases. Further evaluation is necessary.

  5. Accepted 15 March 2012Available online 20 May 2012%Insecticidal, antimicrobial and antioxidant activities of bulb extracts of Allium sativum

    Institute of Scientific and Technical Information of China (English)

    Balaji Meriga; Ramgopal Mopuri; T MuraliKrishna

    2012-01-01

    ABSTRACT Objective:To evaluate the insecticidal, antimicrobial and antioxidant activities of bulb extracts ofAllium sativum(A. sativum).Methods:Dried bulbs ofA. sativum were extracted with different solvents and evaluated for insecticidal, antimicrobial and antioxidant activities.Results:Aqueous and methanol extracts showed highest insecticidal activity (mortality rate of81% and 64% respectively) against the larvae ofSpodoptera litura (S. litura) at a concentration of1 000 ppm. With regard to antimicrobial activity, aqueous extract exhibited antibacterial activity against gram positive (Bacillus subtilis, Staphylococcus aureu,) and gram negative (Escherichia coliandKlebsiella pneumonia) strains and antifungal activity againstCandida albicans. While methanol extract showed antimicrobial activity against all the tested micro organisms except two (Staphylococcus aureus andCandida albicans), the extracts of hexane, chloroform and ethyl acetate did not show any anti microbial activity. Minimum inhibitory concentration of aqueous and methanol extracts against tested bacterial and fungal strains was100-150 μg/mL. Antioxidant activity of the bulb extracts was evaluated in terms of inhibition of free radicals by 2, 2’-diphenly-1-picrylhydrazyl. Aqueous and methanol extracts exhibited strong antioxidant activity(80%-90% of the standard).Conclusions: Antioxidant and antimicrobial activity of A. sativum against the tested organisms therefore, provides scientific basis for its utilization in traditional and folk medicine. Also, our results demonstrated the insecticidal efficacy ofA. sativum againstS. litura, a polyphagous insect.

  6. Assessment of the potential of Allium sativum oil as a new medicament for non-vital pulpotomy of primary teeth

    Science.gov (United States)

    Mohammad, Shukry Gamal; Baroudi, Kusai

    2015-01-01

    Objective: The objective of this study was to compare the clinical and radiographic effects of Allium sativum oil and formocresol in nonvital pulpotomy in primary teeth. Materials and Methods: Twenty children ranging in age from 4 to 8 years were included in the study. In every one of those children, pulpotomy was indicated for the primary molars. Pulpotomy procedure was performed and the radicular pulp tissue of one molar was capped with A. sativum oil in a cotton pellet while the other molar was capped with formocresol. The teeth were evaluated clinically and radiographically before and after 6 months using standard clinical and radiographic criteria. Statistically, these results revealed significant difference between the radiographic findings of nonvital pulpotomy in primary molars with the two medicaments. Statistical analysis was performed using independent t-test and paired t-test at the significance level of α = 0.05. Results: A. sativum oil has potent antibacterial properties that enable it to combat intracanal microbes in the infected pulp of primary molars. Better results were obtained when A. sativum oil was used. Conclusion: A. sativum oil had more powerful effects than formocresol on the infected pulp of primary nonvital molars. PMID:26312232

  7. Coriander volatile oil inhibits growth and migration of Saos-2 cells in vitro%香菜挥发油体外抑制Saos-2细胞生长与迁移

    Institute of Scientific and Technical Information of China (English)

    赖家玲; 付文垚; 何欣; 肖川云; 曹俊

    2016-01-01

    Objective To extract coriander volatile oil and investigate its effects on growth and migration of human Saos-2 cells . Methods Ultrasound-assisted ethanol extraction was used to prepare coriander volatile oil. MTT assay was used to determine the growth inhibion of Saos-2 cells by volatile oil. Cell migra-tion was examined using Boyden chamber assay. Expression of 21 1/ 1, 27 1, 23 1 and 100 4 were examined by real-time PCR. Results The results showed that the yield of coriander volatile oil was 0.424% using ultrasound-assisted ethanol extraction. Coriander volatile oil inhibited the growth and migra-tion of Saos-2 cells, along with the decrease in the expression levels of 21 1/ 1, 27 1 and 100 4. Conclusions Coriander volatile oil can inhibit Saos-2 cell growth and migration in vitro, which may involve 21 1/ 1, 27 1 and 100 4.%目的:探讨香菜挥发油对Saos-2肿瘤细胞株体外生长及迁移的影响。方法采用超声波辅助醇提法制备香菜挥发油;MTT法检测香菜挥发油对Saos-2细胞生长抑制作用;Transwell趋化小室检测细胞迁移抑制作用;定量PCR检测细胞内211/1、271、231和1004的表达变化。结果使用超声辅助醇提法,香菜挥发油得率为0.424%;香菜挥发油可明显抑制Saos-2细胞的生长与迁移,伴随细胞内211/1及271的升高及1004降低,显示具有较强的抗成骨肉瘤活性。结论香菜挥发油可体外抑制Saos-2细胞生长及迁移,其可能机制与211/1、271及1004有关。

  8. Produção de coentro cultivado com esterco bovino e adubação mineral Yield of coriander cultivated with cattle manure and mineral fertilization

    Directory of Open Access Journals (Sweden)

    Ademar P. Oliveira

    2002-09-01

    Full Text Available Avaliou-se o efeito de doses de esterco bovino na presença e ausência de adubo mineral sobre o rendimento do coentro, cv. Verdão. O experimento foi conduzido na UFPB, entre março e maio/2001, no delineamento de blocos casualizados, com os tratamentos distribuídos em esquema fatorial 5 x 2, correspondendo às doses de esterco bovino (0; 2,0; 4,0; 6,0 e 8,0 kg/m² e, presença e ausência de adubo mineral (10 g/m² de P2O5, 6,0 g/m² K2O e 50 g/m² de N, em quatro repetições. A altura das plantas aumentou com a elevação das doses de esterco bovino apenas quando na presença de adubação mineral, ocorrendo incremento na ordem de 0,42 e 1,47 cm na altura, aos 20 e 40 dias, respectivamente, a cada quilograma de esterco bovino adicionado ao solo. A dose de 3,8 kg/m² de esterco bovino, na presença do adubo mineral, proporcionou número máximo de molhos (49,0, enquanto que na ausência de adubo mineral o número de molhos, aumentou com a elevação das doses de esterco bovino, na ordem de 3,0 molhos para cada quilograma de esterco bovino adicionado ao solo. O rendimento máximo estimado de massa verde (5,0 kg/m² foi obtido com 3,9 kg/m² de esterco bovino na presença do adubo mineral, enquanto que na ausência de adubo mineral o rendimento de coentro, aumentou com a elevação das doses de esterco bovino, na ordem de 0,24 kg/m² a cada quilograma de esterco bovino adicionada ao solo.The effect of different levels of cattle manure was evaluated in the presence and absence of mineral fertilizer on coriander, cv. Verdão production. The experimental plots were placed at Universidade Federal da Paraíba, in Areia, Brazil, from March to May 2001. The experiment was set up in a randomized block design, with treatments distributed in a 5 x 2 factorial scheme, corresponding to levels of cattle manure (0; 2.0; 4.0; 6.0 and 8.0 kg m-2 and presence or absence of mineral fertilization (10 g/m² of P2O5, 6.0 g/m² of K2O and 50 g/m² N, with four

  9. Computational Modelling of Pisum Sativum L. Superoxide Dismutase and Prediction of Mutational Variations through in silico Methods

    Directory of Open Access Journals (Sweden)

    Nathan Vinod Kumar

    2014-06-01

    Full Text Available Superoxide dismutase (SOD is one of the major enzymes expressed in the oxidative stress pathway in plants. Its expression is also evident in other taxonomic group in oxidative reactions. Pisum sativum a common plant is being studied in the present work where SOD is characterized using computational tools. SOD sequence of P. sativum [CAA42737.1] Ala and Leu rich protein with alkaline pI value was used as query sequence and used to obtain nine similar sequences through BLASTp. Phylogenetic tree was constructed using MEGA 5.0 based on neighbour joining method. Physiochemical parameters and amino acid composition was studied and compared with query sequences and other similar sequences. Secondary structures were predicted to understand the dominant components. Homology modeling of P. sativum SOD was done using SWISS MODEL and quality was evaluated using standard methods. 27 active sites were detected in SOD predicted model which were Lys rich.

  10. Effect of 2 MHz ultrasound on DNA, RNA, and protein synthesis in Pisum sativum root meristem cells

    Energy Technology Data Exchange (ETDEWEB)

    Miller, M.W.; Ciaravino, V.; Allen, D.; Jensen, S.

    1976-01-01

    The amounts of DNA, RNA and protein synthesis were determined in Pisum sativum root meristem cells at various times after a 1 min exposure to 1 MHz ultrasound at a power density of 30 W/cm/sup 2/. Immediate depressions in all three macromolecular syntheses occurred after sonication, followed by an apparent recovery several hours later. These events appear to correlate in time with the subsequent reduction and recovery in mitotic index in Pisum sativum root meristem cells exposed to 2 MHz ultrasound.

  11. Analysis of nucleolar pre-rRNA processing sites in pea(Pisum sativum)

    Institute of Scientific and Technical Information of China (English)

    LONG; Hong; (龙; 鸿); ZENG; Xianlu; (曾宪录); JIAO; Mingda; (焦明大); HU; Bo; (胡; 波); SUN; Haijing; (孙海晶); LIU; Zhenlan; (刘振兰); ZHANG; Liyong; (张立勇); HAO; Shui; (郝; 水)

    2003-01-01

    The location of rRNA processing was analyzed by using in situ hybridization with ITS1 probe and immunolabeling of anti-fibrillarin mAb in pea (Pisum sativum) root pole cells. The results showed that rRNA processing sites were in dense fibrillar components (DFCs) and granular components (GCs), but not in fibrillar centers (FCs). Low doses of actinomycin D (AMD) treatment can selectively suppress pre-rRNA synthesis but cannot disturb the processing of preformed pre-rRNAs. With AMD treatment prolonged, the density of labeled signals gradually decreased, indicating the preformed pre-rRNAs were gradually processed.

  12. ANTIOKSIDACIJSKA AKTIVNOST OZIMOGA SLAVONSKOGA ČEŠNJAKA (ALLIUM SATIVUM L.)

    OpenAIRE

    2012-01-01

    Brojna istraživanja provedena na češnjaku (Allium sativum L.) dokazala su prisutnost antioksidacijskih i fenolnih spojeva, nekoliko sumpornih spojeva i vitamina. Cilj ovog istraživanja bio je ispitati razlike u sadržaju ukupnih fenola, askorbinske kiseline i ukupne antioksidacijske aktivnosti četiri kultivara ozimoga slavonskoga češnjaka te utvrditi postojanje korelacija između tih parametara. Sadržaj askorbinske kiseline, ukupni sadržaj fenolnih spojeva te ukupna antioksidacijska aktivnos...

  13. Effect of sodium fluoride on phytase activity during germination of Pisum sativum L. seeds

    Energy Technology Data Exchange (ETDEWEB)

    Hauskrecht, I.

    1972-01-01

    The effects of fluoride ion, in concentrations varying from 0 to 4 mM F/sup -/, on phytase activity in the course of germination of Pisum sativum L. seeds and in the course of incubation in vitro are reported. The fluoride ion in 1 mM concentration cause a significant 80% decrease of the phytase activity in the incubation medium and a 24% decrease in the course of germination. The inhibition of phytase, as well as its synthesis, by fluoride ion during germination may influence the metabolism of phosphorus at the initial stage of ontogenesis.

  14. Exposure of Vicia faba and Pisum sativum to copper-induced genotoxicity.

    Science.gov (United States)

    Souguir, D; Ferjani, E; Ledoigt, G; Goupil, P

    2008-11-01

    The potential genotoxicity of Cu(2+) was investigated in Vicia faba and Pisum sativum seedlings in hydroponic culture conditions. Cu(2+) caused a dose-dependent increase in micronuclei frequencies in both plant models. Cytological analysis of root tips cells showed clastogenic and aneugenic effects of this heavy metal on V. faba root meristems. Cu(2+) induced chromosomal alterations at the lowest concentration used (2.5 mM) when incubated for 42 h, indicating the potent mutagenic effect of this ion. A spectrum of chromosomal abnormalities was observed in V. faba root meristems, illustrating the genotoxic events leading to micronuclei formation.

  15. Nitrogen and phosphorus compounds in the aleurone grains of Iris pseudoacorus endosperm and Pisum sativum cotyledons

    Directory of Open Access Journals (Sweden)

    Ligia Konopska

    2015-05-01

    Full Text Available Aleurone grains from Iris pseudoacorus endosperm and Pisum sativum cotyledons were isolated partly according to Tombs's method (1967. Nitrogen compounds content was determined in them by Kjeldahl's micromethod, and in the particular fractions after Thiman and Laloraya (1960. Mainly protein N was detected in the aleurone grains, constituting 14.8 and 15.2 per cent of the dry mass of pea and Iris seeds, respectively. Moreover, phosphorus compounds were fractionated according to Holden and Pirie (1955. Analyses demonstrated the presence in aleurone grains of inorganic P, acid-soluble organophosphorus compounds, phospholipids and RNA.

  16. 河西冷凉灌区芫荽制种技术%Hybrid Seed Production Techniques of Coriandrum stativum in Cool Irrigation Region

    Institute of Scientific and Technical Information of China (English)

    王继平

    2006-01-01

    芫荽(Coriandrum sativum)又名香菜、胡荽、香荽。为伞形花笠、芜荽属,一至二年生草本植物,其鲜嫩茎叶有特殊香味,是人们日常生活主要的调味蔬菜之一。种子含油量达20%以上,既是提炼芳香油的重要原料,也被广泛应用于中药制剂。甘肃河西冷凉灌区适宜芫荽制种,种子产量可达2250-2700kg/hm2,产值为12750元/hm2左右。现将河西冷凉灌区芫荽制种技术介绍如下。

  17. 贮藏温度对鲜切香菜营养成分和抗氧化活性的影响%Effects of storage temperature on nutritional compositions and antioxidant activity of fresh-cut coriander

    Institute of Scientific and Technical Information of China (English)

    韩聪; 李晓安; 高梵; 金鹏; 郑永华

    2015-01-01

    Objective To analyze the effect of different storage temperature on the nutritional compositions and antioxidant activity of fresh-cut coriander.Methods Fresh-cut coriander was cut and placed into preservation boxes, and stored at 4℃ , 10℃ and 20℃ . The changes of ascorbic acid, chlorophyll, soluble sugar, total phenolics, total flavonoids and DPPH radical scavenging activity were investigated.ResultsThe results indicated that cutting induced a fast loss of ascorbic acid, chlorophyll and soluble sugar. Compared with 10℃ and 20℃ , storage temperature of 4℃ significantly decreased the disruption of these nutrients. In addition, cutting also induced the synthesis and accumulation of phenolic and flavone compounds, enhanced its DPPH radical scavenging activity, and improved the antioxidant activity of coriander. Compared with 4℃ and 10℃ , the content of phenolic and flavone compounds and antioxidant activity in fresh-cut coriander were higher at 20℃ .Conclusion Low storage temperature can preserve the nutritional quality of fresh-cut coriander, while higher temperature can induce the improvement of antioxidant activity of fresh-cut coriander.%目的:分析不同温度贮藏对鲜切香菜营养成分和抗氧化活性的影响。方法将香菜进行切割处理后装入保鲜盒,在4℃、10℃和20℃下贮藏,测定鲜切香菜抗坏血酸、叶绿素、可溶性糖、总酚、总黄酮含量和DPPH自由基清除率的变化。结果切割伤害会促进香菜抗坏血酸、叶绿素和可溶性糖含量迅速下降,与10℃和20℃相比,4℃低温贮藏的香菜抗坏血酸等营养成分的消耗速率最低。此外,切割还会诱导香菜多酚和类黄酮类物质的合成、积累及DPPH自由基清除率的上升,从而增强其抗氧化活性。与4℃和10℃相比,20℃条件下香菜的多酚类和类黄酮类物质积累最多,抗氧化活性最强。结论低温贮藏有利于维持鲜切香菜的营养成分,而高温贮藏会

  18. Effect of sodium fluoride and sodium nitroprouside on Cicer arietinum and Pisum sativum

    Directory of Open Access Journals (Sweden)

    Naba Kumar Mondal

    2016-07-01

    Full Text Available In present study, the individual and combine effect of sodium fluoride (NaF and sodium nitroprouside (SNP on germination and biochemical parameters (pigments, sugar, protein, amino acid, and phenol of Bengal gram (Cicer arietinum and peas (Pisum sativum has been studied. After three days of NaF treatment, reductions were observed in percentage of seed germination, root and shoot length, and pigment content with increasing concentration of NaF (1 to 4 mg L-1. Seedlings treated with SNP, both alone and in combination of NaF, showed enhancement in seed germination as well as other growth parameters. NaF-treated seedlings were found to accumulate more soluble sugars and phenols, which were further increased by SNP treatment thereby indicating a synergistic effect of the possible reasons for the ameliorative effects of SNP in seedlings of Pisum sativum growing under NaF stress. Results also demonstrated that SNP application did not show any improvement in both morpho-physiologically and biochemically under sodium fluoride stress condition.

  19. Rhizobium anhuiense sp. nov., isolated from effective nodules of Vicia faba and Pisum sativum.

    Science.gov (United States)

    Zhang, Yu Jing; Zheng, Wen Tao; Everall, Isobel; Young, J Peter W; Zhang, Xiao Xia; Tian, Chang Fu; Sui, Xin Hua; Wang, En Tao; Chen, Wen Xin

    2015-09-01

    Four rhizobia-like strains, isolated from root nodules of Pisum sativum and Vicia faba grown in Anhui and Jiangxi Provinces of China, were grouped into the genus Rhizobium but were distinct from all recognized species of the genus Rhizobium by phylogenetic analysis of 16S rRNA and housekeeping genes. The combined sequences of the housekeeping genes atpD, recA and glnII for strain CCBAU 23252(T) showed 86.9 to 95% similarity to those of known species of the genus Rhizobium. All four strains had nodC and nifH genes and could form effective nodules with Pisum sativum and Vicia faba, and ineffective nodules with Phaseolus vulgaris, but did not nodulate Glycine max, Arachis hypogaea, Medicago sativa, Trifolium repens or Lablab purpureus in cross-nodulation tests. Fatty acid composition, DNA-DNA relatedness and a series of phenotypic tests also separated these strains from members of closely related species. Based on all the evidence, we propose a novel species, Rhizobium anhuiense sp. nov., and designate CCBAU 23252(T) ( = CGMCC 1.12621(T) = LMG 27729(T)) as the type strain. This strain was isolated from a root nodule of Vicia faba and has a DNA G+C content of 61.1 mol% (Tm).

  20. Evaluation of Cytogenetic Effects of Isoproturon on the Root Meristem Cells of Allium sativum

    Institute of Scientific and Technical Information of China (English)

    L.K.S.ChAUHAN; P.N.SAXENA; 等

    2001-01-01

    Objective:To validate the use of Allium sativum sativum as a reliable test model for gentoxicity,isoproturon,a substituted phenylalkylures herbicide,was evaluated on the root meristem cells by this plant system.ethod :Test concentrations were selected by determining EC50 and root tips were exposed to various concentrations for 6 or 24hr.EC50 concentration was calculated to be 70.8ppm for the root growth.In addition on root growth retardation exposure to isoproturon induced morpholoogical changes like discoloruation and stiffness of roots.Results:Exposure to various experimental concentrations of isoproturon(35-280ppm),including EC50,significantly and dose-dependently inhibited the mitotic index and induced chromosome breaks/mitotic aberrations at 6 or 24 hr.Conclusion:The frequency of aberrations was found to be decreased in a dose dependant manner at 24 or 48hr post exposure.howerver in comparison of control cells the frequency of aberrations was considerably high which indicates genotoxicity potentials of isoproturon.Further,present study also suggests that Allium satiuvum is a sensitive,efficient,and reliable test system for measuring the genotoxicity potential of environmental chemicals.

  1. Evaluation of Cytogenetic Effects of Isoproturon on the Root Meristem Cells of Allium sativum

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective To validate the use of Allium sativum as a reliable test model for genotoxicity, isoproturon, a substituted phenylalkylurea herbicide, was evaluated on the root meristem cells by this plant system. Method Test concentrations were selected by determining EC50 and root tips were exposed to various concentrations for 6 or 24 hr. EC50 concentration was calculated to be 70.8 ppm for the root growth. In addition to root growth retardation exposure to isoproturon induced morpholoogical changes like discolouration and stiffness of roots. Results Exposure to various experimental concentrations of isoproturon (35-280 ppm), including EC50, significantly and dose-dependently inhibited the mitotic index and induced chromosome breaks/mitotic aberrations at 6 or 24 hr. Conclusion The frequency of aberrations was found to be decreased in a dose dependant manner at 24 or 48 hr post exposure, however in comparison of control cells the frequency of aberrations was considerably high which indicates genotoxicity potentials of isoproturon. Further, present study also suggests that Allium sativum is a sensitive, efficient, and reliable test system for measuring the genotoxicity potential of environmental chemicals.

  2. Growth and some physiological attributes of pea (Pisum sativum L.) as affected by salinity.

    Science.gov (United States)

    Najafi, F; Khavari-Nejad, R A; Rastgar-Jazii, F; Sticklen, M

    2007-08-15

    The effects of salt stress were studied on growth and physiology of pea (Pisum sativum L. cv. Green Arrow) in a pot study. Pea plants were treated with NaCl at 0, 10, 30, 50 and 70 mM in Hoagland solution. Plants were harvested after 21 days for measurements of physiological parameters. The highest NAR and RGR were found in 10 mM NaCl. However, in 70 mM NaCl, RGR and RLGR were significantly decreased in respect of other concentrations of NaCl. In 50 and 70 mM NaCl, chlorophylls contents and photosynthetic rate, were significantly decreased and CO2 compensation concentration and respiration rate increased in comparison with control. In 10 and 30 mM NaCl gas exchanges and chlorophyll contents were not significantly decrease in respect of control. Results indicated that Pisum sativum L. cv. Green Arrow can tolerate to 70 mM NaCl, also growth of plants in 10 and 30 mM NaCl was better than that of those in 0 mM NaCl.

  3. In vitro anthelminthic activity of alcoholic extract of Allivum Sativum against rumen amphistome

    Directory of Open Access Journals (Sweden)

    G. R. Ghangale

    Full Text Available The present investigation was undertaken to evaluate effect of alcoholic extract of allivum sativum for anthelminthic activity Invitro against rumen amphistome. Mature and healthy worms randomly collected from rumen papillae of freshly slaughtered buffaloes from deonar abattor in normal physiological solution (tyrode solution, having temp (37 0C.The study was conducted at four different dilutions of extracts viz. 5,15,50,75 mg/ml prepared in tyrode solution. Albendazole 5 mg/ml and tyrode solution serves as positive control and negative control respectively. Each of the concentration selected was taken in total volume of 20 ml tyrode solution in a separate clean petridish. Ten vigorously motile worms irrespective of their sex were placed in each petridish and observation were made after every 15 min. for cessation of motility by pinch method mortality was assessed by slightly warming the petridish and observing the movements. The time taken for cessation of motility in alcoholic extract was 3 hour, 1hour, 10min., and instant at 5,15,50,75 mg/ml concentration respectively. Mortality was observed at 4hr, 1.5 hr, 15 min. and instantly respectively in test groups. Time taken for 100% mortality was 6 hour by standard drug.So it was concluded that alcoholic extract of Allivum Sativum is having good activity against worms’ motility Invitro. [Vet World 2009; 2(10.000: 385-386

  4. Allium sativum Compared to Cilostazol as an Inhibitor of Myointimal Hyperplasia

    Science.gov (United States)

    Lima, Paulo Roberto da Silva; Bandeira, Francisco Chavier Vieira; Rolim, Janio Cipriano; Nogueira, Manuel Ricardo Sena; Pordeus, Mizael Armando Abrantes; de Oliveira, Andressa Feitosa Bezerra; Pitta, Guilherme Benjamin Brandão

    2016-01-01

    Objective Intimal hyperplasia is associated with graft failure and vascular sutures in the first year after surgery and in postangioplasty restenosis. Allium sativum (common garlic) lowers cholesterol and has antioxidant effects; it also has antiplatelet and antitumor properties and, therefore, has great potential to reduce or inhibit intimal hyperplasia of the arteries. Our objective is to determine if the garlic has an efficacy to inhibit myointimal hyperplasia compared to cilostazol. Methods Female New Zealand rabbits were divided into the following groups (n=10 each) according to treatment: group A, garlic, 800 µg×kg-1×day-1, orally; group C, cilostazol, 50 mg.day-1, orally; group PS, 10 ml of 0.9% physiological saline solution, orally. Our primary is the difference of the mean of myointimal hyperplasia. Statistical analysis was performed by using ANOVA and Tukey tests, as well as the Chi-square test. We calculated the 95% confidence interval for each point estimate, and the P value was set as < 0.05. Results Group PS had a mean hyperplasia rate of 35.74% (95% CI, 31.76–39.71%); group C, 16.21% (95% CI, 13.36–19.05%); and group A, 21.12% (95% CI, 17.26–25.01%); P<0.0001. Conclusion We conclude that Allium sativum had the same efficacy in inhibiting myointimal hyperplasia when compared to the positive control, cilostazol. PMID:27849301

  5. Allium sativum Compared to Cilostazol as an Inhibitor of Myointimal Hyperplasia

    Directory of Open Access Journals (Sweden)

    Paulo Roberto da Silva Lima

    Full Text Available Abstract Objective: Intimal hyperplasia is associated with graft failure and vascular sutures in the first year after surgery and in postangioplasty restenosis. Allium sativum (common garlic lowers cholesterol and has antioxidant effects; it also has antiplatelet and antitumor properties and, therefore, has great potential to reduce or inhibit intimal hyperplasia of the arteries. Our objective is to determine if the garlic has an efficacy to inhibit myointimal hyperplasia compared to cilostazol. Methods: Female New Zealand rabbits were divided into the following groups (n=10 each according to treatment: group A, garlic, 800 µg×kg-1×day-1, orally; group C, cilostazol, 50 mg.day-1, orally; group PS, 10 ml of 0.9% physiological saline solution, orally. Our primary is the difference of the mean of myointimal hyperplasia. Statistical analysis was performed by using ANOVA and Tukey tests, as well as the Chi-square test. We calculated the 95% confidence interval for each point estimate, and the P value was set as < 0.05. Results: Group PS had a mean hyperplasia rate of 35.74% (95% CI, 31.76–39.71%; group C, 16.21% (95% CI, 13.36–19.05%; and group A, 21.12% (95% CI, 17.26–25.01%; P<0.0001. Conclusion: We conclude that Allium sativum had the same efficacy in inhibiting myointimal hyperplasia when compared to the positive control, cilostazol.

  6. Genetic interaction and mapping studies on the leaflet development (lld) mutant in Pisum sativum

    Indian Academy of Sciences (India)

    Sushil Kumar; Raghvendra Kumar Mishra; Arvind Kumar; Swati Chaudhary; Vishakha Sharma; Renu Kumari

    2012-12-01

    In Pisum sativum, the completely penetrant leaflet development (lld) mutation is known to sporadically abort pinnae suborgans in the unipinnate compound leaf. Here, the frequency and morphology of abortion was studied in each of the leaf suborgans in 36 genotypes and in presence of auxin and gibberellin, and their antagonists. Various lld genotypes were constructed by multifariously recombining lld with a coch homeotic stipule mutation and with af, ins, mare, mfp, tl and uni-tac leaf morphology mutations. It was observed that the suborgans at all levels of pinna subdivisions underwent lld-led abortion events at different stages of development. As in leafblades, lld aborted the pinnae in leaf-like compound coch stipules. The lld mutation interacted with mfp synergistically and with other leaf mutations additively. The rod-shaped and trumpet-shaped aborted pea leaf suborgans mimicked the phenotype of aborted leaves in HD-ZIP-III-deficient Arabidopsis thaliana mutants. Suborganwise aborted morphologies in lld gnotypes were in agreement with basipetal differentiation of leaflets and acropetal differentiation in tendrils. Altogether, the observations suggested that LLD was the master regulator of pinna development. On the basis of molecular markers found linked to lld, its locus was positioned on the linkage group III of the P. sativum genetic map.

  7. Calcium-calmodulin signalling is involved in light-induced acidification by epidermal leaf cells of pea, Pisum sativum L.

    NARCIS (Netherlands)

    Elzenga, JTM; Staal, M; Prins, HBA

    1997-01-01

    Pathways of signal transduction of red and blue light-dependent acidification by leaf epidermal cells were studied using epidermal strips of the Argenteum mutant of Pisum sativum. In these preparations the contribution of guard cells to the acidification is minimal. The hydroxypyridine nifedipine, a

  8. Effect of clinorotation on the leaf mesophyll structure and pigment content in Arabidopsis thaliana L. and Pisum sativum L.

    Science.gov (United States)

    Adamchuk, N I

    2004-07-01

    Properties of mesophyll cells and photosynthetic membranes of Arabidopsis thaliana (L.) Heynh. and Pisum sativum (L.) plants grown in a horizontal clinostat and in control conditions were compared. Obtained data have show that under clinorotation conditions seedlings have experienced the following cell morphology changes structural chloroplast rearrangement in palisade cells, pigment content alteration, and cell aging acceleration.

  9. Characterization of low-strigolactone germplasm in pea (Pisum sativum L.) resistant to crenate broomrape (Orobanche crenata Forsk.)

    NARCIS (Netherlands)

    Pavan, Stefano; Schiavulli, Adalgisa; Marcotrigiano, Angelo Raffaele; Bardaro, Nicoletta; Bracuto, Valentina; Ricciardi, Francesca; Charnikhova, Tatsiana; Lotti, Concetta; Bouwmeester, Harro; Ricciardi, Luigi

    2016-01-01

    Crenate broomrape (Orobanche crenata Forsk.) is a devastating parasitic weed threatening the cultivation of legumes around the Mediterranean and in theMiddle East. So far, only moderate levels of resistance were reported to occur in pea (Pisum sativum L.) natural germplasm, and most commercial cu

  10. Micronuclei formation in Pisum sativum L. root meristem cells exposed to an electric field or. gamma. -rays

    Energy Technology Data Exchange (ETDEWEB)

    Miller, M.W.; Economou, P.; Cox, C.; Robertson, D.

    1982-01-01

    Gamma radiation (400 R, 800 R; acute exposre), but not a 430 V/m 60 Hz electric field (continuous 5 day exposure in culture medium with a conductivity of 0.08 S/m), induced micronuclei formation in root meristem cells of Pisum sativum L.

  11. Isolation and characterization of N-feruloyltyramine as the P-selectin expression suppressor from garlic (Allium sativum)

    Science.gov (United States)

    Because garlic (Allium sativum) is believed to have positive health effects on cardiovascular disease, the screening of isolated fractions from a garlic extract against cardiovascular disease related-processes should help identify active compounds. Both P-selectin expression suppressing activity ag...

  12. Clinical and Radiographic Evaluation of Allium sativum Oil as a New Medicament for Vital Pulp Treatment of Primary Teeth

    Science.gov (United States)

    Mohammad, Shukry Gamal; Raheel, Syed Ahmed; Baroudi, Kusai

    2014-01-01

    Background: The objective of this study was to compare between the clinical and radiographic effects of Allium sativum oil and those of formocresol in vital pulpotomy in primary teeth. Materials and Methods: A total of 20 children age ranged from 4 to 8 years were included in the study. In every one of those children, the primary molars indicated for pulpotomy. Pulpotomy procedure was performed, and the radicular pulp tissue of one molar capped with A. sativum oil in a cotton pellet, whereas the other molar capped with formocresol, the teeth evaluated clinically and radiographically before and after 6 months, using standard clinical and radiographical criteria. Statistically, these results revealed no significant difference between the radiographic findings of vital pulpotomy in primary molars with the two medicaments was found. Results: A. sativum oil offers a good healing potential, leaving the remaining pulp tissue healthy and functioning. Vital pulpotomy with allium sativa oil was given raise 90% success rate while that with formocresol was 85%. Conclusion: A. sativum oil is a biocompatible material that is compatible with vital human pulp tissue. It offers a good healing potential, leaving the remaining pulp tissue healthy and functioning. PMID:25628480

  13. AcEST: BP915195 [AcEST

    Lifescience Database Archive (English)

    Full Text Available sterase, chloroplastic (Fragment) OS=Coriandrum sativum Align length 118 Score (bit) 112.0 E-value 2.0e-24 R...2 >sp|Q42712|FATA_CORSA Oleoyl-acyl carrier protein thioesterase, chloroplastic (Fragment) OS=Coriandrum sat

  14. Variabilidade genética em progênies de meios-irmãos de coentro Genetic variability of half-sib progenies of coriander

    Directory of Open Access Journals (Sweden)

    Roberto de A Melo

    2009-09-01

    Full Text Available Grande número de produtores envolve-se com o cultivo do coentro durante todo o ano, tornando a cultura importante social e economicamente. Praticamente em toda a região Nordeste utiliza-se a cultivar Verdão. Estudos da variabilidade genética do coentro são importantes, tendo em vista o melhor planejamento de programas de melhoramento genético. Desta forma, este trabalho teve como objetivo quantificar a variabilidade genética de características agronômicas do coentro cultivar Verdão, avaliando-se progênies de meios-irmãos potencialmente úteis no melhoramento genético. O trabalho foi desenvolvido na Universidade Federal Rural de Pernambuco, em casa de vegetação, em blocos casualizados, com cinco repetições e parcelas de 28 plantas, colocadas em dois vasos. Os tratamentos foram compostos por 55 progênies de meios-irmãos da cultivar Verdão. O teste t detectou significância a 1 e a 5% de probabilidade entre as correlações genotípicas, fenotípicas e ambientais. A herdabilidade no sentido amplo variou de 7,19 (peso médio de plantas a 81,09 (número de plantas pendoadas. Para a razão entre os coeficientes de variação genético e ambiental (CVg /CVe, obteve-se de 0,27 (peso médio de plantas a 2,07 (número de plantas pendoadas, indicando que a seleção contra o pendoamento apresenta as condições mais favoráveis em termos de ganhos genéticos imediatos. A correlação genotípica entre altura de plantas não pendoadas e número de plantas pendoadas foi alta e significativa.A large number of producers are involved in the cultivation of coriander throughout the year in Brazil, making it a crop of social and economic importance. Throughout nearly the entire Northeast Region, cultivar Verdão is used. Genetic variability studies on coriander are important for the proper planning of genetic improvement programs. The aim of the present study was to quantify the genetic variability for agronomic characteristics in cv. Verd

  15. Genetic similarity between coriander genotypes using ISSR markers Similaridade genética entre genótipos de coentro por marcadores ISSR

    Directory of Open Access Journals (Sweden)

    Roberto de A Melo

    2011-12-01

    Full Text Available With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16, resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão, at 52%. The highest similarity was found between Português and Palmeira, at 75%. The ISSR is efficient for identifying DNA polymorphism in coriander.Com o surgimento de novas cultivares, uma caracterização genética precisa é essencial, visando à utilização em programas de melhoramento ou para fins de registros e ou proteção de cultivares. Marcadores moleculares vêm complementando a caracterização morfológica e agronômica tradicional, uma vez que são virtualmente ilimitados, cobrem todo o genoma e não são influenciados pelo ambiente. A caracterização genética constitui a base para

  16. Respuesta fisiológica del cilantro a diferentes niveles de potasio y nitrógeno Physiological response of coriander to different levels of potassium and nitrogen

    Directory of Open Access Journals (Sweden)

    María Sara Mejía de Tafur

    2008-10-01

    Full Text Available Se realizó un experimento con el fin de evaluar la respuesta del cilantro cv. Unapal-Precoso a diferentes niveles de N y K. Se sembraron plantas en potes con 4 kg de arena de río, lavada, y se regaron con soluciones nutritivas (Testigo: solución completa de Hougland y Arnon; tratamientos restantes: concentraciones variables de N y K. Se utilizó un diseño completamente al azar con siete tratamientos y cinco repeticiones. Se realizaron cuatro muestreos cada nueve días hasta la cosecha final. Con la concentración alta de K se observó mayor acumulación de biomasa y menor pérdida de agua en poscosecha. El rango óptimo de concentración de N en la solución nutritiva se estimó entre 3.9 y 8.7 mM y el nivel crítico de deficiencia de K en 8.4 mM.To evaluate the response of coriander cv. Unapal-Precoso at different levels of N and K an experiment was carried out. Plants were sown in pots with 4 kg of river washed sand and irrigated with nutrient solutions (Control: solution of Hougland and Arnon, other treatments: different concentrations of N and K. A complete randomized design with seven treatments and five repetitions was used. Four sampling every nine days until the final harvest were taken. With the high concentration of K greater biomass accumulation and the lowest post-harvest loss of water was observed. The optimum range of N concentration in the nutrient solution was estimated between 3.9-8.7 mM and the critical level of K deficiency was 8.4 mM.

  17. In vitro investigation of the toxic effects of extracts of Allium sativum bulbs on adults of Hyalomma marginatum rufipes and Rhipicephalus pulchellus

    Directory of Open Access Journals (Sweden)

    F. Nchu

    2005-06-01

    Full Text Available The toxic effects of the extracts of Allium sativum (Garlic were evaluated against adults of Hyalomma marginatum rufipes and Rhipicephalus pulchellus using three types (Types A, B and C of contact toxicity bioassays. A. sativum bulbs were extracted with acetone, ethanol and dichloromethane (DCM solvents. Among these three solvents, it is the DCM extract of A. sativum that appears to have anti-tick activity. In the Type A contact toxicity bioassay, DCM extracts of A. sativum demonstrated a high acaricidal bioactivity against H. m. rufipes with 100 % of ticks killed in less than an hour, and toxicity persisted to the second day. A weak acaricidal activity of aqueous extracts of A. sativum was observed in the Type B contact toxicity bioassay. In the Type C contact toxicity bioassay, a concentration of 24 % w/v of DCM extracts of garlic in sunflower oil (Helianthus annuus had killed 100% of H. m. rufipes (LC50= 5.9 % w/v and R. pulchellus (LC50 = 10.3 % w/v by 24 hours post-treatment of ticks. The results obtained from this study suggest that DCM extract of A. sativum is a potential source of novel acaricidal agents.

  18. Cultivar and Rhizobium Strain Effects on the Symbiotic Performance of Pea (Pisum sativum)

    DEFF Research Database (Denmark)

    Skøt, Leif

    1983-01-01

    content and dry weight/N ratio. At harvest 63 days after planting, cultivars accounted for 75% of the variation in dry weight, while the Rhizobium strains accounted for 63% of the variation in N-content and 70% of the variation in dry weight/N ratio. Cultivar × strain interactions were statistically......The symbiotic performance of four pea (Pisum sativum L.) cultivars in combination with each of four strains of Rhizobium leguminosarum was studied in growth chamber experiments in order to estimate the effects of cultivars, strains and cultivar × strain interaction on the variation in dry weight, N...... significant, but of minor quantitative importance, accounting for 5–15% of the total variation. Rhizobium strains also influenced the partitioning of N between reproductive and vegetative plant parts and between root and shoot biomass....

  19. Nitrogen deficiency hinders etioplast development in stems of dark-grown pea (Pisum sativum) shoot cultures.

    Science.gov (United States)

    Kósa, Annamária; Preininger, Éva; Böddi, Béla

    2015-11-01

    The effects of nitrogen (N) deprivation were studied in etiolated pea plants (Pisum sativum cv. Zsuzsi) grown in shoot cultures. The average shoot lengths decreased and the stems significantly altered considering their pigment contents, 77 K fluorescence spectra and ultrastructural properties. The protochlorophyllide (Pchlide) content and the relative contribution of the 654-655 nm emitting flash-photoactive Pchlide form significantly decreased. The etioplast inner membrane structure characteristically changed: N deprivation correlated with a decrease in the size and number of prolamellar bodies (PLBs). These results show that N deficiency directly hinders the pigment production, as well as the synthesis of other etioplast inner membrane components in etiolated pea stems. © 2015 Scandinavian Plant Physiology Society.

  20. The effect of Allium sativum on ischemic preconditioning and ischemia reperfusion induced cardiac injury

    Directory of Open Access Journals (Sweden)

    Bhatti Rajbir

    2008-01-01

    Full Text Available In the present study, the effect of garlic (Allium sativum extract on ischemic preconditioning and ischemia-reperfusion induced cardiac injury has been studied. Hearts from adult albino rats of Wistar strain were isolated and immediately mounted on Langendorff′s apparatus for retrograde perfusion. After 15 minutes of stabilization, the hearts were subjected to four episodes of 5 min ischemia, interspersed with 5 min reperfusion (to complete the protocol of ischemic preconditioning, 30 min global ischemia, followed by 120 min of reperfusion. In the control and treated groups, respective interventions were given instead of ischemic preconditioning. The magnitude of cardiac injury was quantified by measuring Lactate Dehydrogenase and creatine kinase concentration in the coronary effluent and myocardial infarct size by macroscopic volume method. Our study demonstrates that garlic extract exaggerates the cardio protection offered by ischemic preconditioning and per se treatment with garlic extract also protects the myocardium against ischemia reperfusion induced cardiac injury.

  1. Photosynthesis and growth responses of pea Pisum sativum L. under heavy metals stress

    Institute of Scientific and Technical Information of China (English)

    Sabrine Hattab; Boutheina Dridi; Lassad Chouba; Mohamed Ben Kheder; Hamadi Bousetta

    2009-01-01

    The present work aims to study the physiological effects of cadmium (Cd) and copper (Cu) in pea (Pisum sativum).Pea plants were exposed to increasing doses of cadmium chloride (CdCl_2) and copper chloride (CuCl_2) for 20 d.The examined parameters,namely root and shoot lengths,the concentration of photosynthetic pigments and the rate of photosynthesis were affected by the treatments especially with high metals concentrations.The analysis of heavy metals accumulation shows that leaves significantly accumulate cadmium for all the tested concentrations.However,copper was significantly accumulated only with the highest tested dose.This may explain the higher inhibitory effects of cadmium on photosynthesis and growth in pea plants.These results are valuable for understanding the biological consequences of heavy metals contamination particularly in soils devoted to organic agriculture.

  2. A Comprehensive Review on Nickel (II And Chromium VI Toxicities - Possible Antioxidant (Allium Sativum Linn Defenses

    Directory of Open Access Journals (Sweden)

    Kusal K.Das

    2009-12-01

    Full Text Available The toxicity associated with nickel (II and chromium (VI is mainly due to generation of reactive oxygen species (ROS with subsequent oxidative deterioration of biological macromolecules. Both nickel and chromium can generate free radicals (FR directly from molecular oxygen in a two step process to produce superoxide anion and in continued process, produce highly toxic hydroxyl radical. The pro-oxidative effects are compounded by fact that they also inhibit antioxidant enzymes and deplete intracellular glutathione. Garlic (Allium sativum has played an important dietary and medicinal role throughout the history of mankind. Garlic has the potential to enhance the endogenous antioxidant status in nickel as well as hexavalent chromium induced lipid peroxidation in normal and diabetic rats.

  3. Identification of the 64 kilodalton chloroplast stromal phosphoprotein as phosphoglucomutase. [Pisum sativum

    Energy Technology Data Exchange (ETDEWEB)

    Salvucci, M.E.; Drake, R.R.; Broadbent, K.P.; Haley, B.E. (Univ. of Kentucky, Lexington (USA)); Hanson, K.R.; McHale, N.A. (Connecticut Agricultural Experiment Station, New Haven, CT (USA))

    1990-05-01

    Phosphorylation of the 64 kilodalton stromal phosphoprotein by incubation of pea (Pisum sativum) chloroplast extracts with ({gamma}-{sup 32}P)ATP decreased in the presence of Glc-6-P and Glc-1,6-P{sub 2}, but was stimulated by glucose. Two-dimensional gel electrophoresis following incubation of intact chloroplasts and stromal extracts with ({gamma}-{sup 32}P)ATP, or incubation of stromal extracts and partially purified phosphoglucomutase (EC 2.7.5.1) with ({sup 32}P)Glc-1-P showed that the identical 64 kilodalton polypeptide was labeled. A 62 kilodalton polypeptide was phosphorylated by incubation of tobacco (Nicotiana sylvestris) stromal extracts with either ({gamma}-{sup 32}P)ATP or ({sup 32}P)Glc-1-P. In contrast, an analogous polypeptide was not phosphorylated in extracts from a tobacco mutant deficient in plastid phosphoglucomutase activity. The results indicate that the 64 (or 62) kilodalton chloroplast stromal phosphoprotein is phosphoglucomutase.

  4. On the shock response of Pisum Sativum (a.k.a the Common Pea)

    Science.gov (United States)

    Leighs, James; Hazell, Paul; Appleby-Thomas, Gareth

    2011-06-01

    The high strain-rate response of biological and organic structures is of interest to numerous fields ranging from the food industry (dynamic pasteurisation) to astrobiology (e.g. the theory of panspermia, which suggests that planets could be `seeded' with life `piggy-backing' of interplanetary bodies). Consequently, knowledge of the damage mechanisms and viability of shocked organic material is of paramount importance. In this study a single-stage gas-gun has been employed to subject samples of Pisum Sativum (the Common Pea) to semi-planar shock loading, corresponding to impact pressures of up to c.3 GPa. The experimental approach adopted is discussed along with results from Manganin gauges embedded in the target capsule which show the loading history. Further, the viability of the shock-loaded peas was investigated via attempts at germination. Finally, microscopic examination of the impacted specimens allowed a qualitative assessment of damage mechanisms to be made.

  5. Subcellular localization of Cd in the root cells of Allium sativum by electron energy loss spectroscopy

    Indian Academy of Sciences (India)

    Donghua Liu; Ingrid Kottke

    2003-06-01

    The ultrastructural investigation of the root cells of Allium sativum L. exposed to three different concentrations of Cd (100 M, 1 mM and 10 mM) for 9 days was carried out. The results showed that Cd induced several significant ultrastructural changes – high vacuolization in cytoplasm, deposition of electron-dense material in vacuoles and nucleoli and increment of disintegrated organelles. Data from electron energy loss spectroscopy (EELS) revealed that Cd was localized in the electron-dense precipitates in the root cells treated with 10 mM Cd. High amounts of Cd were mainly accumulated in the vacuoles and nucleoli of cortical cells in differentiating and mature root tissues. The mechanisms of detoxification and tolerance of Cd are briefly explained.

  6. SSR genetic linkage map construction of pea(Pisum sativum L.) based on Chinese native varieties

    Institute of Scientific and Technical Information of China (English)

    Xuelian; Sun; Tao; Yang; Junjie; Hao; Xiaoyan; Zhang; Rebecca; Ford; Junye; Jiang; Fang; Wang; Jianping; Guan; Xuxiao; Zong

    2014-01-01

    Simple sequence repeat(SSR)markers have previously been applied to linkage mapping of the pea(Pisum sativum L.)genome.However,the transferability of existing loci to the molecularly distinct Chinese winter pea gene pool was limited.A novel set of pea SSR markers was accordingly developed.Together with existing SSR sequences,the genome of the G0003973(winter hardy)×G0005527(cold sensitive)cross was mapped using 190 F2individuals.In total,157 SSR markers were placed in 11 linkage groups with an average interval of 9.7 cM and total coverage of 1518 cM.The novel markers and genetic linkage map will be useful for marker-assisted pea breeding.

  7. Organic fertilization alters the community composition of root associated fungi in Pisum sativum

    DEFF Research Database (Denmark)

    Yu, L.; Nicolaisen, M.; Ravnskov, S.

    2013-01-01

    Organic fertilization is well known to affect individual functional groups of root associated fungi such as arbuscular mycorrhizal (AM) fungi and root pathogens, but limited information is available on the effect of organic fertilization at the fungal community composition level. The main objective...... of the present study was to examine the response of communities of root associated fungi in Pisum sativum to Protamylasse, an organic fertilizer used in pea production. Plants were grown in pots with field soil amended with four different levels of Protamylasse. 454 pyrosequencing was employed to examine......, the organic fertilizer Protamylasse clearly affects communities of root associated fungi, which seems to be linked to the life strategy of the different functional groups of root associated fungi. --------------------------------------------------------------------------------...

  8. Antioxidant activity of garlic essential oil (Allium Sativum) grown in north Indian plains

    Institute of Scientific and Technical Information of China (English)

    Reena lawrence; Kapil lawrence

    2011-01-01

    Objective: To assess in vitro antioxidant activity of the essential oil isolated from fresh rhizomes of garlic (Allium sativum) of the family Alliaceae in an yield of 0.2% (v/w). Methods:2, 2-diphenyl-1-picrylhydrazyl (DPPH), Nitrogen oxide scavenging, reducing power and毬-carotene bleaching assays were conducted. BHT and gallic acid were kept as standards.Results:IC50 values observed for DPPH and nitric oxide scavenging assays were 0.5 mg/mL and 50毺g/mL respectively. In reducing power assay absorbance increased linearly with increasing concentration of the oil, in毬-carotene bleaching method also there is 84% bleaching in first one hour and it decreased to 45 % by the completion of second hour. Conclusions: The results clearly indicate garlic essential oil is effective in scavenging free radical and has the potential to be powerful antioxidant.

  9. [Meiotic abnormalities as expression of nuclear-cytoplasmic incompatibility in crosses of Pisum sativum subspecies].

    Science.gov (United States)

    Bogdanova, V S; Galieva, E R

    2009-05-01

    Meiosis in anthers and mitosis in somatic cells were studied in reciprocal F1 hybrids of the accession VIR320, which belonged to wild Pisum sativum ssp. elatius (Bieb.) Schmal., and the laboratory line Sprint-1. When VIR320 was used as a maternal form, the hybrids displayed nuclear-cytoplasmic conflict, which caused chlorophyll defects and meiotic abnormalities. One or two chromosomes lagged in the equatorial region during chromosome segregation to the poles, distorting cytokinesis and yielding abnormal microspores. Chlorophyll defects were not observed, and meiotic abnormalities were far less frequent in reciprocal hybrids and in the case of an abnormal paternal inheritance of plastids from Sprint-1. Mitosis lacked overt abnormalities in all of the hybrids.

  10. Survival of Bifidobacterium bifidum in cow- and camel-milk yogurts enriched with Cinnamomum verum and Allium sativum

    Directory of Open Access Journals (Sweden)

    Amal Bakr Shori

    2015-10-01

    Full Text Available The effects of Allium sativum and Cinnamomum verum water extracts on the survival of Bifidobacterium bifidum during 21 days of refrigerated storage and after simulated gastrointestinal digestion (SGD were investigated. Two types of yogurt (cow- and camel-milk yogurts were prepared in the presence of A. sativum or C. verum. The viable cell counts (VCC of B. bifidum in fresh A. sativum- or C. verum-cow milk yogurt (1 day were higher (8.1 × 109 cfu/ml and 6.6 × 109 cfu/ml, respectively; p < 0.05 than plain-yogurt (1.9 × 109 cfu/ml. In contrast, B. bifidum VCC in fresh plain-camel milk yogurt was 1.99 × 109 cfu/ml whereas the presence of A. sativum or C. verum in yogurt increased (p < 0.05 VCC to 19.61 × 109 cfu/ml and 25.55 × 109 cfu/ml, respectively. The VCC of B. bifidum in both herbal-yogurts decreased (p < 0.05 during refrigerated storage for both types of yogurt. The VCC of B. bifidum was ∼1.3 × 109 cfu/ml in all fresh cow milk yogurts after 1 h gastric digestion. Intestinal digestion (1 h increased VCC of B. bifidum in all fresh yogurts but not in 7 day old yogurts (plain- and A. sativum-yogurts. However, prolonged digestion to another 1 h in intestine reduced (p < 0.05 VCC of B. bifidum in all fresh and storage yogurts. In contrast, all fresh camel milk yogurts showed VCC of B. bifidum ⩽1 × 109 cfu/ml after SGD. Seven day old A. sativum – camel milk yogurt showed the lowest survival of B. bifidum after gastric digestion compared to plain- and C. verum-yogurt. The VCC reduced (p < 0.05 in all camel milk-yogurts after 2 h intestinal digestion.

  11. Shelf Life Extension of Maple pea (Pisum sativum var. arvense L. Spread Using Sous Vide

    Directory of Open Access Journals (Sweden)

    Ķirse Asnate

    2016-12-01

    Full Text Available The aim of this study was to determine the effect of sous vide packaging on the shelf life of maple pea (Pisum sativum var. arvense L. spread. Pea spreads were made of ground re-hydrated cooked maple peas ‘Bruno’ (Pisum sativum var. arvense L., to which salt, citric acid, oil, and spices were added. Pea spread was stored in polyamide/polyethylene (PA/PE film pouches, packaged in vacuum and hermetically sealed. Pea spread pouches were heat treated in a water bath, then rapidly cooled in ice-water and stored at 4.0 ± 0.5 °C. Sous vide was applied in three different heat regimens +(65.0; 80.0 and 100.0 ± 0.5 °C with cooking times 5, 10, 15, 20, 25, and 30 min at a constant temperature. Total plate count was determined according to ISO 4833-1:2014 on Plate Count Agar and Enterobacteriaceae determination was performed in accordance with ISO 21528-2:2004 on Violet Red Bile Glucose Agar. Total plate count in pea spread without thermal treatment was 3.41 log10 CFU g−1, in all sous vide packaged pea spread samples microbial contamination was significantly lower (p < 0.05. Enterobacteriaceae were not detected in any samples. It is possible to extend the shelf life of sous vide maple pea spread up to 14 weeks when stored at 4.0 ± 0.5 °C.

  12. Sperm immobilization activity of Allium sativum L. and other plant extracts

    Institute of Scientific and Technical Information of China (English)

    KausikiChakrabarti; SulagnaPal; AsokK.Bhattacharyya

    2003-01-01

    Aim: To identify possible spermicidal agents through screening a number of edible medicinal plants with antimicrobial activity. Methods: Initial screening was made on the basis of ram cauda epididymal sperm immobiliza-tion immediately after addition of extracts. The most potent extract was selected and was evaluated on both ram and human spermatozoa. To unravel its mode of action several sperm functional tests were carried out, namely viability of cells, hypo-osmotic swelling test for membrane integrity and assays of membrane-bound enzyme 5'-nucleotidase and acrosomal marker enzyme acrosin. Results: The crude aqueous extract of the bulb ofAllium sativum L. Showed the most promising results by instant immobilization of the ram epididymal sperm at 0.25 g/mL and human ejaculated sperm at 0.5 g/mL. Sperm immobilizing effects were irreversible and the factor of the extract responsible for immobilization was thermostable up to 90℃. On boiling at 100℃ for 10 minutes, this activity was markedly reduced. Moreover, this extract was able to cause aggregation of ram sperms into small clusters after 30 minutes of incubation at 37℃. However this property was not found in human spermatozoa. More than 50 % reduction in sperm viability and hypo-osmotic swelling occurred in treated sperm as compared with the controls, indicating the possibility of plasma membrane disintegration which was further supported by the significant reduction in the activity of membrane bound 5''-nucleotidase and acrosomal acrosin. Conclusion: The crude aqueous extract of A. Sativum bulb possesses spermicidal activity in vitro. (Asian J Androl 2003 Jun, 5:131-135 )

  13. Chemical composition, antibacterial and antioxidant activities of Tunisian garlic (Allium sativum essential oil and ethanol extract

    Directory of Open Access Journals (Sweden)

    Raja Zouari Chekki

    2014-09-01

    Full Text Available The aim of the study is to establish some nutritional properties of garlic cultivated in Tunisia and to evaluate the antioxidant and the antimicrobial activites of its essential oil and ethanol extract. Tunisian garlic (Allium sativum was characterized for moisture, ash and protein contents which were determined as 66%, 1.4% and 5.2% respectively. In addition, Fe (5.90 mg/kg, Cu (1.61 mg/kg, Mg (15 mg/kg and P (140 mg/kg were reported such as the major minerals in garlic. The fat profile of tunisian garlic was conducted, the main fatty acids identified were lauric acid (49.3% and linoleic acid (20.4%. Essential oil obtained from A. sativum was analysed by capillary GCMS. Diallyl disulfide (49.1% and diallyl trisulfide (30.38% were the main components of the five identified components. The phenolic content of The ethanol extract are analysed for its phenolic profiles, colorimetric analysis revealed that the total phenols, flavonoids and proanthocyanidins contents were respectively 43.63 mg GA/g, 13.18 mg quercetin/g and 24.24 mg of catechin/g. Antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl assay, essential oil presented the highest antioxidant activity compared to its ethanolic extract. IC50 values observed for the essential oil and ethanol extract were 300 μg/ml and 600 µg/ml respectively. The essential oil and ethanol extract from raw garlic were tested for antimicrobial activity against seven microorganisms. The results showed that ethanol extract was active against all tested strains: Escherichia coli, Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogene, Yersinia enterocolitica and Bacillus cereus.

  14. Antimicrobial evaluation, acute and sub-acute toxicity studies of Allium sativum

    Institute of Scientific and Technical Information of China (English)

    Bashir Lawal; Oluwatosin Kudirat Shittu; Florence Inje Oibiokpa; Hadiza Mohammed; Sheriff Itopa Umar; Garba Muhammed Haruna

    2016-01-01

    Objective: To evaluate the antimicrobial and toxicological effects of aqueous garlic (Allium sativum) bulbs extract in wister rat using biochemical and hematological parameters. Methods: A total of 20 wister rats were assigned into four (A–D) groups of five animals each. Group A served as the control and was administered 1 mL of distilled water. Groups B–C were given 300, 600 and 1 200 mg/kg body weight of garlic (Allium sativum) bulbs extract for 5 weeks. Results: Garlic bulbs extract produced significant inhibitory activities against all bacteria tested at concentrations of 120 and 160 mg/mL. However, at concentration of 80 mg/mL, the extract had no inhibitory activities against Klebsiella pneumoniae and Salmonella typhi. The minimal inhibitory concentration and minimal bactericidal concentration ranged between 80–120 mg/mL and 120–160 mg/mL respectively. Toxicological study revealed that the extract did not cause any significant (P > 0.05) alteration to serum aspartate transaminase, alkaline phosphatase activities, total bilirubins, Na, K, creatinine, red blood cell, hematocrit, hemoglobin, mean corpuscular hemoglobin concentration, granulocyte and organs-body weight ratio. However, serum alanine transaminase activ-ities, total proteins, direct bilirubins, Cl− concentrations and body weight gain were significantly (P<0.05) lowered while the concentrations of urea, albumin, white blood cell, mean corpuscular hemoglobin and mean corpuscular volume count were raised significantly (P<0.05) in rats dosed with 600 and 1 200 mg/kg of the extract. However, at a dose of 300 mg/kg only, the concentrations of Cl−, urea and albumin were mildly altered. Conclusions: The extract caused selective changes in some biochemical parameters of organ function;however, since only mild alteration was observed at a dose of 300 mg/kg, the garlic bulb may be considered to be relatively safe and could be explored as an oral remedy at this dose.

  15. 超临界CO2萃取芫荽籽油的数学模型%Research on the Mathematical Model of the Extraction of Coriander Seed Oil with Supercritical Carbon Dioxide Fluid

    Institute of Scientific and Technical Information of China (English)

    成江晨; 白凌

    2011-01-01

    [目的]优化超临界CO2萃取芫荽籽油的工艺条件.[方法]通过单因子试验考察萃取压力、CO2流量、萃取时间以及萃取温度对芫荽籽油萃取率的影响.利用MATLAB软件,对试验数据进行多元多项式拟合.[结果]对纯粹二次拟合模型进行拟合可信度的F检验,结果表明多项式各项对y线性关系极显著(P<0.01),从而确定萃取压力、CO2流量、萃取时间以及萃取温度对萃取率影响的较为合理的拟合模型为纯粹二次多项式模型.将上述二次多项式代入MATLAB的无约束最优化工具,计算出超临界CO2萃取芫荽籽油的最佳工艺条件是:萃取压力21.84 MPa,CO2流量33.26 L/h,萃取时间142.90 min,萃取温度42.6℃,该条件下最佳萃取率为12.61%.[结论]超临界CO2萃取芫荽籽油工艺中,萃取压力、CO2流量、萃取时间、萃取温度对萃取率的影响是相互独立的,通过对各工艺条件的改变可以大幅度提高芫荽籽油萃取率.%[ Objective] The process condition of the extraction of coriander seed oil with the method of supercritical carbon dioxide fluid was optimized. [Method] The effect of the extracting pressure, time and temperature, the rate of CO2 flow, on the yield of coriander seed oil based on the method was investigated through the single factor experimental design. The experimental data was fitted in multiply-element and multiply-item/polynomial by means of MATLAB software. [ Result] The reliability of the purely quadratic fitting model was done with F-test and the testing results showed that the item of multiply-item/polynomial was significant with the y linear relationship with (P < 0.01 ), so it was determined that more reasonable fitting model of the effect of the extraction pressure, the flow rate of carbon dioxide, the extraction time and the extraction temperature on the extraction rate was the purely quadratic multiply-item/polynomial model. After the quadratic multiply-item/polynomial was put

  16. Produção e valor agroeconômico no consórcio entre cultivares de coentro e de alface Yield and agroeconomic values through intercropping of coriander and lettuce cultivars

    Directory of Open Access Journals (Sweden)

    Eliane Q. de Oliveira

    2005-06-01

    Full Text Available O experimento foi realizado de outubro a dezembro de 2003 em campo, na ESAM, com o objetivo de avaliar agroeconomicamente cultivares comerciais de coentro consorciadas com cultivares de alface. O delineamento experimental usado foi de blocos completos casualizados em esquema fatorial 5 x 2, com três repetições. Os tratamentos consistiram da combinação de cinco cultivares de coentro (Verdão, Supéria, Português, Asteca e Santo com duas cultivares de alface (Tainá e Babá de Verão. Avaliaram-se a altura de plantas, número de hastes/planta, massa seca da parte aérea, número de molhos comerciáveis/m² e rendimento estimado de massa verde no coentro, e, altura e diâmetro de plantas, número de folhas/planta, massa seca da parte aérea e produtividade estimada na alface. Alguns indicadores agroeconômicos foram usados para medir a eficiência biológica e econômica dos sistemas consorciados. Houve interação entre as cultivares de alface e de coentro na altura de plantas, número de molhos/m² e no rendimento estimado de massa verde do coentro, com a cultivar de coentro Português registrando a melhor performance produtiva quando combinada com ambas as cultivares de alface. As cultivares de alface tiveram desempenho produtivo diferente, com a cultivar Babá de Verão significativamente superior à Tainá. Em todos os sistemas consorciados foram observadas eficiências agroeconômicas, porém as maiores foram registradas nos consórcios entre as cultivares Tainá e Asteca, e Babá de Verão e Português.The experiment was carried from October to December 2003, in the field, to evaluate the production and agrieconomic performance of coriander cultivars intercropped with lettuce cultivars. The experimental design was of randomized complete blocks in a 5 x 2 factorial scheme, with three replications. The treatments consisted of the combination of five coriander cultivars (Verdão, Superia, Português, Asteca and Santo with two lettuce

  17. Ultrasonic Extraction of Coriander(cilantro) Leaf Essential Oil and its NaNO2 Scavenging Activity%超声波提取芫荽(香菜)茎叶挥发油及其对NaNO2的清除作用的研究

    Institute of Scientific and Technical Information of China (English)

    廖晓峰; 于荣

    2013-01-01

    This artical focuses on extracting coriander leaf volatile oil through the ultrasonic method to research the influences of the extraction efficiency of coriander oil under the different ultrasound extracting power, time, and temperature. The experimental result is the best extraction process combination which are 25℃extracting temperature, 60 W extracting power, and 100 min extracting time. The yield of coriander oil is 0.285%. Also, researches the extracting coriander oil's Nitrite eliminating rate under the simulated gastric juice condition. The result showed that coriander leaf volatile oil can efficiently eliminating Nitrite under the simulated gastric juice condition, and the eliminating rate is 72.72%.%通过超声波法提取香菜茎叶挥发油,研究超声波提取功率、时间、温度对香菜精油提取率的影响,实验结果是提取最佳工艺组合为:提取温度25℃,提取功率60 W,提取时间100 min,香菜精油得率为0.285%。将提取的香菜茎叶挥发油用于对在模拟胃液条件下亚硝酸盐消除率的研究。实验结果表明,香菜茎叶挥发油能有效清除模拟胃液条件下的亚硝酸盐,清除率为72.72%。

  18. Effect of garlic (Allium sativum on growth factors, some hematological parameters and body compositions in rainbow trout (Oncorhynchus mykiss

    Directory of Open Access Journals (Sweden)

    Amin Farahi

    2010-12-01

    Full Text Available The aim of this study was to assess the effect of garlic (Allium sativum on growth factors,some hematological parameters and body compositions in rainbow trout (Oncorhynchus mykiss. A totalnumber of 360 fish (average weight 20.88±0.25 g was used. Fish were divided into four groups fed ondiets containing garlic in different levels; 10 g kg-1, 20 g kg-1, 30 g kg-1 diet and the control group dietwas without garlic. The experiment extended for two months. The results showed that, weight gain andgrowth performance of O. mykiss significantly (p-1 diet of garlic than all other groups. Total lipids content in fish body decreased in treatments and itwas lower in fish fed on 30 g kg-1 diet of garlic. The results of this study show that addition of garlicAllium sativum to fish diet can promote growth and improve fish health.

  19. The potential role of garlic (Allium sativum) against the multi-drug resistant tuberculosis pandemic: a review.

    Science.gov (United States)

    Dini, Catia; Fabbri, Alessia; Geraci, Andrea

    2011-01-01

    Worldly data show the increasing incidence of Mycobacterium tuberculosis (MTB) and particularly of multi-drug resistant tuberculosis (MDR-TB). In developing countries, TB control programmes are overwhelmed by the complexity of treating MDR-TB infected people, as current tools and therapies are inadequate. MDR-TB could become the main form of TB. Risk factors that make South Africa into one of the main epicentres are analysed. A review of the studies carried out about antitubercular properties of Allium sativum both in vitro and in vivo is provided. The researches about the garlic extracts effectiveness against clinical isolates of MDR-TB are of scientific importance. Allium sativum offers a hope for developing alternative drugs. The involvement of traditional healers (TH) in the TB health management could facilitate the administration of garlic extracts to the infected patients.

  20. The potential role of garlic (Allium sativum against the multi-drug resistant tuberculosis pandemic: a review

    Directory of Open Access Journals (Sweden)

    Catia Dini

    2011-12-01

    Full Text Available Worldly data show the increasing incidence of Mycobacterium tuberculosis (MTB and particularly of multi-drug resistant tuberculosis (MDR-TB. In developing countries, TB control programmes are overwhelmed by the complexity of treating MDR-TB infected people, as current tools and therapies are inadequate. MDR-TB could become the main form of TB. Risk factors that make South Africa into one of the main epicentres are analysed. A review of the studies carried out about antitubercular properties of Allium sativum both in vitro and in vivo is provided. The researches about the garlic extracts effectiveness against clinical isolates of MDR-TB are of scientific importance. Allium sativum offers a hope for developing alternative drugs. The involvement of traditional healers (TH in the TB health management could facilitate the administration of garlic extracts to the infected patients.

  1. Low-angle X-ray scattering from spices

    Energy Technology Data Exchange (ETDEWEB)

    Desouky, O.S. E-mail: omardesouky@yahoo.com; Ashour, Ahmed H.; Abdullah, Mohamed I.; Elshemey, Wael M

    2002-07-01

    Low-angle scattering of X-rays is characterized by the presence of one or more peaks in the forward direction of scattering. These peaks are due to the interference of photons coherently scattered from the molecules of the medium. Thus these patterns are closely linked to the molecular structure of the investigated medium. In this work, low-angle X-ray scattering (LAXS) profiles of five spices; pimpinella anisum (anise), coriandrum sativum (coriander), cuminum cyminum (cumin), foenculum vulgare (fennel) and nigella sativa (nigella or black cumin) are presented after extensive measurements. It is found that all spices exhibit one characteristic peak at a scattering angle around 10 deg. This is equivalent to a value x=0.0565 A{sup -1}, where x=sin({theta}/2)/{lambda}. The full width at half maximum (FWHM) of this peak is found to be characteristic for each type of the investigated spices. The possibility to detect the irradiation of these spices from their LAXS profiles is also examined after 10, 20, 30 and 40 kGy doses of gamma radiation. Except for anise, coriander and cumin at 40 kGy, there are no detectable deviations from the control samples in the scattering profiles of irradiated samples. These results comply with the recommendations of the FDA (US Food and Drug Administration) which defines 30 kGy as the maximum dose for irradiation of spices. The present technique could be used to detect over-irradiation, which causes damage to the molecular structure of some spices.

  2. Antimicrobial activity of plant essential oils against bacterial and fungal species involved in food poisoning and/or food decay.

    Science.gov (United States)

    Lixandru, Brînduşa-Elena; Drăcea, Nicoleta Olguţa; Dragomirescu, Cristiana Cerasella; Drăgulescu, Elena Carmina; Coldea, Ileana Luminiţa; Anton, Liliana; Dobre, Elena; Rovinaru, Camelia; Codiţă, Irina

    2010-01-01

    The currative properties of aromatic and medicinal plants have been recognized since ancient times and, more recently, the antimicrobial activity of plant essential oils has been used in several applications, including food preservation. The purpose of this study was to create directly comparable, quantitative data on the antimicrobial activity of some plant essential oils prepared in the National Institute of Research-Development for Chemistry and Petrochemistry, Bucharest to be used for the further development of food packaging technology, based on their antibacterial and antifungal activity. The essential oils extracted from thyme (Thymus vulgaris L.), basil (Ocimum basilicum L.), coriander (Coriandrum sativum L.), rosemary (Rosmarinus officinalis L.), sage (Salvia officinalis L.), fennel (Foeniculum vulgare L.), spearmint (Mentha spicata L.) and carraway (Carum carvi L.) were investigated for their antimicrobial activity against eleven different bacterial and three fungal strains belonging to species reported to be involved in food poisoning and/or food decay: S. aureus ATCC 25923, S. aureus ATCC 6538, S. aureus ATCC 25913, E. coli ATCC 25922, E. coli ATCC 35218, Salmonella enterica serovar Enteritidis Cantacuzino Institute Culture Collection (CICC) 10878, Listeria monocytogenes ATCC 19112, Bacillus cereus CIP 5127, Bacillus cereus ATCC 11778, Candida albicans ATCC 10231, Aspergillus niger ATCC 16404, Penicillium spp. CICC 251 and two E. coli and Salmonella enterica serovar Enteritidis clinical isolates. The majority of the tested essential oils exibited considerable inhibitory capacity against all the organisms tested, as supported by growth inhibition zone diameters, MICs and MBC's. Thyme, coriander and basil oils proved the best antibacterial activity, while thyme and spearmint oils better inhibited the fungal species.

  3. Low-angle X-ray scattering from spices

    Science.gov (United States)

    Desouky, Omar S.; Ashour, Ahmed H.; Abdullah, Mohamed I.; Elshemey, Wael M.

    2002-07-01

    Low-angle scattering of X-rays is characterized by the presence of one or more peaks in the forward direction of scattering. These peaks are due to the interference of photons coherently scattered from the molecules of the medium. Thus these patterns are closely linked to the molecular structure of the investigated medium. In this work, low-angle X-ray scattering (LAXS) profiles of five spices; pimpinella anisum (anise), coriandrum sativum (coriander), cuminum cyminum (cumin), foenculum vulgare (fennel) and nigella sativa (nigella or black cumin) are presented after extensive measurements. It is found that all spices exhibit one characteristic peak at a scattering angle around 10°. This is equivalent to a value x=0.0565 Å -1, where x=sin( θ⧸2)⧸ λ. The full width at half maximum (FWHM) of this peak is found to be characteristic for each type of the investigated spices. The possibility to detect the irradiation of these spices from their LAXS profiles is also examined after 10, 20, 30 and 40 kGy doses of gamma radiation. Except for anise, coriander and cumin at 40 kGy, there are no detectable deviations from the control samples in the scattering profiles of irradiated samples. These results comply with the recommendations of the FDA (US Food and Drug Administration) which defines 30 kGy as the maximum dose for irradiation of spices. The present technique could be used to detect over-irradiation, which causes damage to the molecular structure of some spices.

  4. Evaluación preclínica de la actividad hepatoprotectora de Ocimum basilicum L. y Allium sativum L.

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    Deodelsy Bermúdez Toledo

    2014-02-01

    Full Text Available Fundamento: la búsqueda de alternativas de tratamiento naturales, orientadas a proteger el hígado de los efectos nocivos de hepatotoxinas, es un tema importante dentro de las investigaciones médico-farmacéuticas. Objetivo: evaluar preclínicamente la actividad hepatoprotectora de las especies Ocimum basilicum L. y Allium sativum L. en un biomodelo de toxicidad inducido por paracetamol. Métodos: se realizó un estudio farmacológico preclínico para evaluar el efecto hepatoprotector de las especies Ocimum basilicum L. y Allium sativum L. frente a la toxicidad inducida por el paracetamol. Se emplearon ratones adultos machos NMRI a los que se administró por vía oral extractos blandos de las plantas de estudio a dosis de 200mg/kg y 400mg/kg, tres días consecutivos previos a la inducción de la hepatotoxicidad. Se evaluaron los signos clínicos de toxicidad, parámetros bioquímicos hepáticos; también se realizó el análisis morfológico del hígado. Resultados: los parámetros bioquímicos analizados mostraron diferencias altamente significativas, pero solo tres grupos presentaron un comportamiento similar al grupo control no tratado. No se confirmaron alteraciones macroscópicas del hígado. Microscópicamente, predominó la ausencia de alteraciones histopatológicas en los grupos donde se administró Ocimum basilicum L. a ambas dosis y Allium sativum L. a 200mg/Kg; también se observó daño leve al administrar 400mg/Kg de Allium sativum L, con diferencias significativas respecto al grupo control no tratado. Conclusión: los extractos blandos de las especies Ocimum basilicum L. y Allium sativum L. a 200mg/kg, poseen mayor actividad hepatoprotectora frente a la acción tóxica del paracetamol.

  5. Evaluation of Biomass Yield and Water Treatment in Two Aquaponic Systems Using the Dynamic Root Floating Technique (DRF

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    Laura Silva

    2015-11-01

    Full Text Available The experiment evaluates the food production and water treatment of TAN, NO2−–N, NO3−–N, and PO43− in two aquaponics systems using the dynamic root floating technique (DRF. A separate recirculation aquaculture system (RAS was used as a control. The fish cultured was Nile tilapia (Oreochromis niloticus. The hydroponic culture in one treatment (PAK was pak choy (Brassica chinensis, and in the other (COR coriander (Coriandrum sativum. Initial and final weights were determined for the fish culture. Final edible fresh weight was determined for the hydroponic plant culture. TAN, NO2−–N, NO3−–N, and PO43− were measured in fish culture and hydroponic culture once a week at two times, morning (9:00 a.m. and afternoon (3:00 p.m.. The fish biomass production was not different in any treatment (p > 0.05 and the total plant yield was greater (p < 0.05 in PAK than in COR. For the hydroponic culture in the a.m., the PO43− was lower (p < 0.05 in the PAK treatment than in COR, and in the p.m. NO3−–N and PO43− were lower (p < 0.05 in PAK than in COR. The PAK treatment demonstrated higher food production and water treatment efficiency than the other two treatments.

  6. Extraction of volatile oil from aromatic plants with supercritical carbon dioxide: experiments and modeling.

    Science.gov (United States)

    Coelho, Jose P; Cristino, Ana F; Matos, Patrícia G; Rauter, Amélia P; Nobre, Beatriz P; Mendes, Rui L; Barroso, João G; Mainar, Ana; Urieta, Jose S; Fareleira, João M N A; Sovová, Helena; Palavra, António F

    2012-09-05

    An overview of the studies carried out in our laboratories on supercritical fluid extraction (SFE) of volatile oils from seven aromatic plants: pennyroyal (Mentha pulegium L.), fennel seeds (Foeniculum vulgare Mill.), coriander (Coriandrum sativum L.), savory (Satureja fruticosa Béguinot), winter savory (Satureja montana L.), cotton lavender (Santolina chamaecyparisus) and thyme (Thymus vulgaris), is presented. A flow apparatus with a 1 L extractor and two 0.27 L separators was built to perform studies at temperatures ranging from 298 to 353 K and pressures up to 30.0 MPa. The best compromise between yield and composition compared with hydrodistillation (HD) was achieved selecting the optimum experimental conditions of extraction and fractionation. The major differences between HD and SFE oils is the presence of a small percentage of cuticular waxes and the relative amount of thymoquinone, an oxygenated monoterpene with important biological properties, which is present in the oils from thyme and winter savory. On the other hand, the modeling of our data on supercritical extraction of volatile oil from pennyroyal is discussed using Sovová's models. These models have been applied successfully to the other volatile oil extractions. Furthermore, other experimental studies involving supercritical CO(2) carried out in our laboratories are also mentioned.

  7. Extraction of Volatile Oil from Aromatic Plants with Supercritical Carbon Dioxide: Experiments and Modeling

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    Helena Sovová

    2012-09-01

    Full Text Available An overview of the studies carried out in our laboratories on supercritical fluid extraction (SFE of volatile oils from seven aromatic plants: pennyroyal (Mentha pulegium L., fennel seeds (Foeniculum vulgare Mill., coriander (Coriandrum sativum L., savory (Satureja fruticosa Béguinot, winter savory (Satureja montana L., cotton lavender (Santolina chamaecyparisus and thyme (Thymus vulgaris, is presented. A flow apparatus with a 1 L extractor and two 0.27 L separators was built to perform studies at temperatures ranging from 298 to 353 K and pressures up to 30.0 MPa. The best compromise between yield and composition compared with hydrodistillation (HD was achieved selecting the optimum experimental conditions of extraction and fractionation. The major differences between HD and SFE oils is the presence of a small percentage of cuticular waxes and the relative amount of thymoquinone, an oxygenated monoterpene with important biological properties, which is present in the oils from thyme and winter savory. On the other hand, the modeling of our data on supercritical extraction of volatile oil from pennyroyal is discussed using Sovová’s models. These models have been applied successfully to the other volatile oil extractions. Furthermore, other experimental studies involving supercritical CO2 carried out in our laboratories are also mentioned.

  8. Physicochemical properties of honey from Marche, Central Italy: classification of unifloral and multifloral honeys by multivariate analysis.

    Science.gov (United States)

    Truzzi, Cristina; Illuminati, Silvia; Annibaldia, Anna; Finale, Carolina; Rossetti, Monica; Scarponi, Giuseppe

    2014-11-01

    The purpose of this study was the physicochemical characterization and classification of Italian honey from Marche Region with a chemometric approach. A total of 135 honeys of different botanical origins [acacia (Robinia pseudoacacia L.), chestnut (Castanea sativa), coriander (Coriandrum sativum L.), lime (Tilia spp.), sunflower (Helianthus annuus L.), Metcalfa honeydew and multifloral honey] were considered. The average results of electrical conductivity (0.14-1.45 mS cm(-1)), pH (3.89-5.42), free acidity (10.9-39.0 meq(NaOH) kg(-1)), lactones (2.4-4.5 meq(NaOH) kg(-1)), total acidity (14.5-40.9 meq(NaOH) kg(-1)), proline (229-665 mg kg(-1)) and 5-(hydroxy-methyl)-2-furaldehyde (0.6-3.9 mg kg(-1)) content show wide variability among the analysed honey types, with statistically significant differences between the different honey types. Pattern recognition methods such as principal component analysis and discriminant analysis were performed in order to find a relationship between variables and types of honey and to classify honey on the basis of its physicochemical properties. The variables of electrical conductivity, acidity (free, lactones), pH and proline content exhibited higher discriminant power and provided enough information for the classification and distinction of unifloral honey types, but not for the classification of multifloral honey (100% and 85% of samples correctly classified, respectively).

  9. Apiaceae seeds as functional food

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    Aćimović Milica G.

    2015-01-01

    Full Text Available The aim of this review was to point to a great importance of plants from Apiaceae family as a functional food. Caraway (Carum carvi L., anise (Pimpinella anisum L., coriander (Coriandrum sativum L., dill (Anethum graveolens L., fennel (Foeniculum vulgare Mill. and cumin (Cuminum cyminum L. are plants from the above-mentioned family whose seeds are widely used in folk medicine, pharmaceutical industry, as spices, flavoring agents and as dietary supplements. These plants are rich in essential oil, which is a mixture of volatile compounds that give it a characteristic aroma. Their antioxidant and antimicrobial activities have been proven and because of these activities they have great potential to be used as natural food conservatives. These plants also have hypoglycemic and hypolipidemic activities as well as anticancer properties. They are used as food supplements in everyday nutrition and as natural health products for the prevention and treatment of many disorders such as inflammations, hyperglycemia, hyperlipidemia and others. Apart from this, these plants have real application in foods such as pastries, meat and dairy products, pickles and salads as well as spice blends like curry powder, garam masala and others.

  10. New Experimental Hosts of Tobacco streak virus and Absence of True Seed Transmission in Leguminous Hosts.

    Science.gov (United States)

    Vemana, K; Jain, R K

    2010-10-01

    Of 70 plant species tested, 50 species were susceptible to Tobacco streak virus (TSV) on sap inoculation. Both localized (necrotic and chlorotic spots) and systemic (necrotic spots, axillary shoot proliferation, stunting, total necrosis and wilt) symptoms are observed by majority of plant species. Eleven new experimental hosts were identified viz., Amaranthus blitum var. oleracea (Chaulai sag), Celosia cristata (Cocks comb), Beta vulgaris var. bengalensis (Palak/Indian spinach), Calendula officinalis (Pot marigold), Chrysanthemum indicum, Cosmos sulphurens (Yellow cosmos), Citrullus lunatus (Watermelon), Lagenaria siceraria (Bottle gourd), Coriandrum sativum (Coriander), Hibiscus subderiffa var. subderiffa (Roselle) and Portulaca oleraceae (Little hogweed). Detected groundnut seed infection with TSV for the first time by Direct antigen coated immunosorbent assay (DAC-ELISA) using whole seed. The seed infection ranged from 18.9 to 28.9% among the seeds collected from naturally infected and sap inoculated groundnut varieties (JL 24, TMV 2, Prasuna, Kadiri 6, Kadiri 9, Anantha and Kadiri 7 Bold) belonging to spanish and virginia types. Further, TSV was detected both in pod shell and seed testa and none of the samples showed the presence of TSV either in cotyledon or embryo. Grow-out and bio-assay tests proved the absence of seed transmission in groundnut and other legume crops. Hence, TSV isolate was not a true seed transmission case under Indian conditions in legumes.

  11. Effect of Allium cepa and Allium sativum on some immunological cells in rats.

    Science.gov (United States)

    Mirabeau, Tatfeng Y; Samson, Enitan S

    2012-01-01

    Extracts of some spices have been reported to play a contributory role in enhancing immune function. We evaluated and compared the effect(s) of single and combined oral administration of fresh aqueous onion (Allium cepa) and garlic (Allium sativum) extracts at different concentrations on some immunological determinants in rats. CD₄ cells of the rats were estimated using Partec flow cytometric technique, while total and differential white blood cell (WBC) counts were estimated using the Sysmsex® automated haematology analyzing technique. Our findings revealed that, CD4 and total WBC counts were significantly increased (P≤0.05) in a dose-dependent manner in both onion (250mg/Kg/d: 349±11cell/ul and 2.75±0.15X10³cell/l; 500mg/Kg/d: 389±10cells/µl and 3.05±0.05 X10³cell/l; 750mg/Kg/d: 600±11cell/µl and 3.25±0.05X10³cells/l) and garlic (250mg/Kg/d: 410±10cell/ul and 2.85±0.15X10³cell/l; 500mg/Kg/d: 494±32cells/µl and 3.30±0.10 X10³cell/l; 750mg/Kg/d: 684±11cell/µl and 3.55±0.05X10³cells/l) treated rats when compared to the zero control (200±11cells/µl and 1.55±0.05X10³cells/l, respectively). Extract of garlic at 750mg/Kg/d had significantly increased the CD4 cells and total white cell count when compared to other concentrations (P≤0.05). However, no significant effect was observed on these parameters when extracts were combined (250mg/Kg/d: 252±21cell/µl and 1.80±0.10X10³cells/l; 500mg/Kg/d: 315±21cells/ul and 2.10±0.10X10³cells/l; 750mg/Kg/d: 368±10cells/µl and 2.35±0.05X10³cells/l, respectively), the differential WBC count showed a significant increase in the proportion of cell types (lymphocytes, neutophils and monocytes) (P≤0.05). The results from this study revealed the immune boosting capabilities of Allium cepa and Allium sativum, but underscored their synergistic activities.

  12. Survey of insect fauna from plants medicinal, aromatic and seasoning and disinfestation by the process of radiation; Levantamento da entomofauna de plantas medicinais, aromaticas e condimentares e desinfestacao pelo processo de irradiacao

    Energy Technology Data Exchange (ETDEWEB)

    Reis, Fabricio Caldeira

    2013-07-01

    The present study aimed to survey the insect fauna associated with medicinal plants, aromatic dehydrated and seasoning trade in Sao Paulo city, using different doses of gamma radiation with the aim of disinfestation of the material and determine the lethal dose of gamma radiation on Sphaericus gibboides. From April to May 2011 were collected in 10 establishments the following sample materials: Melissa officinalis L. (Lemongrass), Mentha piperita L. (Mint), Ocimum basilicum L. (Basil), Origanum vulgare L. (Oregano), Rosmarinus officinalis L. (Rosemary), Thymus vulgaris L. (Thyme), Senna alexandrina Mill (senna), Coriandrum sativum L. (Coriander), Petroselinum crispum (Mill.) Fuss (salsa) and Pimpinella anisum L. (Fennel), Baccharis trimera (Less.) DC. (Gorse), Chamomilla recutita L. (= M. recutita L.) (chamomile), Laurus nobilis L. (Blonde) (Lauraceae); Capsicum annuum L. (Sweet paprika), Bixa orellana L. (Spice) (Bixaceae) and Peumus boldus Molina (Boldo). The first screening showed that all the tested materials did not show the presence of adult insects. After 45 days 940 adult insects were found and larvae from eggs. The substrates analyzed Chamomilla recutita showed the highest rate of infestation, with 70,6%. Pelmus boldus, Laurus nobilis, Chamomilla recutita and Capsicum annuum, had the highest species diversity. Baccharis trimera, Bixa orellana, Melissa officinalis, Origanum vulgare and Coriandrum sativum showed no infestation. The species was Lasioderma serricorne the insect with the largest number of individuals found (936), higher percentage of infestation in different materials (62.5%) and lots, and highest occurrence (68,75%) materials (M. piperita, S. alexandrian, P. anisum, Chamomilla recutita, P. crispum, L. nobilis, C. sativum, C. annuum, O. basilicum, P. boldus and T. vulgaris). The following materials were selected for testing disinfestation by irradiation process: Bixa orellana, Capsicum annuum, Cassia angustifolia, Coriandrum sativum, Mentha

  13. Produção e renda bruta de mandioquinha-salsa, solteira e consorciada com cenoura e coentro = Yield and gross income of Peruvian carrot in monocrop system and intercropped with carrot and coriander

    Directory of Open Access Journals (Sweden)

    Néstor Antonio Heredia Zárate

    2007-10-01

    Full Text Available Foram estudadas a mandioquinha-salsa ‘Amarela de Carandaí’- M, a cenoura ‘Brasília - Ce o coentro ‘Tipo Português’- Co em cultivos solteiros e os consórcios MCe e MCo. Os cinco tratamentos foram arranjados no campo, no delineamento experimental de blocos casualizados, com cinco repetições. A colheita da cenoura foi aos 95 dias após a semeadura; a do coentro, aos 112 dias e a da mandioquinha-salsa, aos 247 dias após o plantio. A altura das plantas (42,3 cm e as massas frescas de raízes total (9,07 t ha-1 e comercial (6,55 t ha-1 da cenoura no consórcio MCe tiveram aumentos significativos de 4,4 cm; 2,34 e 1,73 t ha-1, respectivamente, em relação às plantas cultivadas solteiras. No coentro, a altura (24,0 cm e a massa fresca de folhas (2,98 t ha-1 das plantas solteiras foram significativamente maiores que as consorciadas. Na mandioquinha-salsa, houve influência significativa da forma de cultivo e os maiores valores de massas frescas, em t ha-1, de folhas(17,84, rebentos (4,07, coroas (3,99 e de raízes totais (14,04, comerciais (10,46 e nãocomerciais (3,58 foram obtidos no cultivo solteiro. O consórcio MCe foi considerado efetivo (RAE = 1,47 e o MCo foi inefetivo (RAE = 0,76. A renda bruta indicou que osdois consórcios não devem ser recomendados para o produtor de mandioquinha-salsa porque induziriam perdas de R$ 8.650,00 e R$ 7.011,25, respectivamente.‘Amarela de Carandaí’ Peruvian carrot (M, ‘Brasília’ carrot (Ce and ‘Tipo Português’ coriander (Co were studied in monocrop system and intercropped with carrot and coriander. Five treatments were arranged at field in a completely randomized block design with five replications. Harvest of carrot occurred 95 days after sowing; coriander harvest occurred 112 days after sowing; and the Peruvian carrot harvest, 247 days after planting. Plant height (42.3 cm and fresh masses of total (9.07 t ha-1 and commercial (6.55 t ha-1 roots of carrot in the MCe intercrop

  14. Yield and gross income of bunching onion and coriander in monocrop and intercrop systems/ Produção e renda bruta de cebolinha e de coentro, em cultivo solteiro e consorciado

    Directory of Open Access Journals (Sweden)

    Cleila Marcondes de Souza

    2005-06-01

    Full Text Available The objective of this work was to determine yield and income of ‘Todo ano’ bunching onion and ‘Asteca’ coriander, which were carried out in monocrop and intercrop systems with three (Ce3 and Co3 and four rows planted per plot. The work was developed in Dourados-MS, Brazil, between July 13th, and September 15th, 2004. Average yield of fresh mass of bunching onion plants under monocrop system had significative increase of 0.64 t ha-1 (92.75% in relation to those under intercrop system (0.69 t ha-1. For coriander, yield of fresh mass of plants under monocrop system and with four rows per plot had significative increase of 1.43 t ha-1 (41.21% in relation to those under three rows (3.47 t ha-1. Land Equivalant Ratio (LER for bunching onion and coriander intercropped were superior in 21% (Ce3Co4 and 56% (Ce4Co3 in relation to monocrop system. For bunching onion producer, both intercrops were economic viable, but Ce4Co3 could have induced a higher gain per hectare (R$ 3,243.30. By the other side, for coriander producer, Ce3Co4 intercrop was the worst, because, although LER had been superior to 1.00, it could induce loss per hectare of R$ 99.42. Ce4Co3 intercrop was the best because it could induce a gain of R$ 777.77.O objetivo do trabalho foi determinar a produtividade e o retorno econômico da cebolinha ‘Todo Ano’ e do coentro ‘Asteca’, conduzidos em cultivo solteiro e consorciado, com três (Ce3 e Co3 e quatro (Ce4 e Co4 linhas de plantas por canteiro. O trabalho foi desenvolvido em Dourados-MS, entre 13 de julho e 15 de setembro de 2004. A produção média de massa fresca das plantas de cebolinha sob cultivo solteiro teve aumento significativo de 0,64 t ha-1 (92,75% em relação àquela sob consórcio (0,69 t ha-1. No coentro, a produção de massa fresca das plantas sob cultivo solteiro e com quatro linhas por canteiro teve aumento significativo de 1,43 t ha-1 (41,21% em relação à produção sob três linhas (3,47 t ha-1. As

  15. Study on purification of flavonoids from coriander with AB-8 macroporous resin%AB -8大孔树脂对芫荽黄酮的纯化工艺研究

    Institute of Scientific and Technical Information of China (English)

    王珊珊; 吴春

    2014-01-01

    利用AB-8大孔树脂纯化芫荽黄酮,通过静态和动态结合的方法,确定最佳工艺参数.结果表明,静态,吸附平衡时间为3 h,解析平衡时间为1.5 h;动态,上样液质量浓度为1.0 mg/mL,上样液pH值为6.0,上样流速为2 mL/min,洗脱剂质量浓度为70%乙醇溶液,洗脱流速为2 mL/min.在此条件下,AB-8大孔树脂可以较好的分离纯化芫荽中总黄酮.%AB-8 macroporous resin was used to separate and purify coriander flavonoids , static and dynamic adsorption and desorption behaviors of AB -8 macroporous resin were e-valuated for achieving the best purification of total flavonoids .The static:adsorption equilib-rium time was 4 h, desorption time was 1.5 h.The dynamic:the best adsorption was sample concentration of 1.0 mg/mL, pH of 6.0 and the flow rate of 2.0 mL/min.The desorption flow rate of 2.0 mL/min and 70% ethanol elution were the best .With definite technical process , the AB-8 macroporous resin could be used to purify the flavonoids of coriander .

  16. Study of DC and AC electric field effect on Pisum sativum seeds growth

    Science.gov (United States)

    Mahmood, Bahar; Jaleh, Sojoodi; Yasaman, Yasaie

    2014-07-01

    In this research the effect of electric field on two groups of wet and dry Pisum sativum seeds growth was studied. To generate the required electric field a parallel-plate capacitor with round copper plates of 30 cm diameter was used. The experiments were performed once in fixed exposure duration of 8 min in variable DC electric field of 0.25-1.5 kV/m. The other experiments were performed in variable fields of 50-125 kV/m in fixed exposure duration of 8 min, in two groups of AC and DC electric fields. The experiments were repeated three times. In each experiment 10 seeds were used and there was a sham exposed group for comparison, too. After application of electric field, the seeds were kept for six days in the same growth chamber with the temperature of 25 ± 1 °C and 12 h light/12 h darkness. On the 6th day length of stems and height of roots were measured. After doing statistical analysis, in low intensities of DC electric field, the highest significant increase of mean growth (The average of stem length and the height of roots) was seen in 1.5 kV/m in wet seeds. In high intensities of DC and AC electric fields, the highest significant increase of mean growth was seen in AC electric field of 100 kV/m in wet seeds.

  17. Efectos alelopáticos de Rumex Crispus L. sobre Pissum Sativum L

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    Moreno B. Luis Francisco

    1989-06-01

    Full Text Available

    Se investigaron los efectos alelopáticos de R. Crispus sobre P. sativum mediante 3 ensayos: el primero consistió en incorporar residuos de diferentes partes de la maleza a un cultivo de arveja: los otros dos, en aplicar extractos acuosos y etéricos, en diferentes concentraciones, a semillas de arveja puestas a germinar. El estudio se realizó bajo un diseño completamente al azar en parcelas divididas. El efecto se evaluó mediante la varianza y pruebas de Desviación Standard Media (DSM. Se halló que la maleza inhibe la germinación,  afecta el crecimiento y aspecto de la radicula e incrementa el crecimiento y acumulación de biomasa. Se concluye que la planta presenta alelopatía, mediante inhibidores presentes en las hojas. Esta investigación es de utilidad práctica en manejo de agroecosisternas. Por su carácter preliminar, plantea nuevas investigaciones que deberán hacerse para completar la información adquirida.

  18. Bactericidal Efficacy of Allium sativum (garlic Against Multidrug Resistant Vibrio cholerae O1 Epidemic Strains

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    Pramod Kumar

    2016-09-01

    Full Text Available In recent years, emerging trend of antibiotic resistance in Vibrio cholerae associated with cholera epidemics is a matter of serious concern for the management of the disease. Indiscriminate use of antibiotics generally results in selection of antibiotic resistant strains. Introduction of newer antibiotics is a challenging task for the researchers as bacteria soon attain resistance. Therefore, identifying natural compounds of medicinal importance for control of cholera would be the best alternative. Garlic (Allium sativum was recognised for many centuries in early Chinese, Egyptian and Indian civilisations as an herbal or traditional medicine. In present study, garlic was selected for screening of antimicrobial efficacy against V. cholerae. A total of 55 V. cholerae strains isolated from various outbreaks/epidemics were subjected to antimicrobial testing as per CLSI, USA 2010 guidelines. Antimicrobial screening of garlic extract was performed against all the multidrug resistant strains of V. cholerae. The garlic extracts showed antibacterial activity against all the V. cholerae strains tested, irrespective of their origin, multidrug resistance and virulence. Antibacterial efficacy of garlic on V. cholerae was also evident from in vivo study on sealed adult mice model. Thus, the Garlic extract harnesses the potential to control infection of multidrug resistant V. cholerae, especially in outbreak like situations in remote and under developed areas where drug supply itself is a challenge

  19. Garlic (Allium sativum: diet based therapy of 21st century–a review

    Directory of Open Access Journals (Sweden)

    Hafiz Ansar Rasul Suleria

    2015-04-01

    Full Text Available Functional and nutraceutical foods provide an opportunity to improve one’s health by reducing health care costs and to support economic development in rural communities. For this reason, various phyto-based functional foods are becoming popular worldwide owing to number of evidences for their safer therapeutic applications. Garlic (Allium sativum L., is an essential vegetable that has been widely utilized as seasoning, flavoring, culinary and in herbal remedies. The consumption of traditional plants especially garlic has progressively increased worldwide because of their great effectiveness, fewer side effects and relatively low cost. Garlic is well known to contain an array of phytochemicals. These bioactive molecules are playing pivotal role in maintaining human health and having potential to reduce various ailments. It has distinct nutritional profile with special reference to its various bioactive components that can be used in different diet based therapies to cure various life-style related disorders. The present review is an attempt to explore the functional/nutraceutical role of garlic against various threats including dyslipidemia and hyperglycemia, cardiovascular disorders, antioxidant capacity and carcinogenic perspectives.

  20. Comportement agronomique d'une collection de pois (Pisum sativum L

    Directory of Open Access Journals (Sweden)

    Bouslama, M.

    2007-01-01

    Full Text Available Agronomical Behaviour of a Pea Collection (Pisum sativum L.. This experience was achieved under greenhouse conditions. Twelve genotypes of pea were used (Asgrow, Jumbo, Lincoln, Merveille de Kelvedon, Purser, Rajai Torpe, Snajor Kosep, Korai,Wando, Rondo, local genotype, Major Kosep Korai and Surgevil. They were cultivated on peat during 5.5 months (from October to April. Some agronomical parameters were studied: resistance to diseases, (Powdery-mildew, mildew, top yellow virus, anthracnose, browning, fresh matter, number of branches/plant, number of flowers/plant, number of pods/ plant and the yield of grains /plant. Results showed that only the genotype Purser is resistant to all diseases and Surgevil is sensitive only to the Top Yellow virus. The local genotype is sensitive to three frequent diseases (Powdery-mildew, mildew and Anthracnose. With regard to vegetative growth, the highest yield of fresh matter do not contribute towards a high fertility rate. In fact, only the genotypes having a weak yield of fresh matter (Snajor Kosep Korai, Asgrow, Major Kosep Korai, Rajai Torpe and Purser have the most important rate of fertility (> 30%. Within this group, the most important yield (> 9 g/plant is a result of high: number of pods/plant (7.5 to 21.6 and of grains/pod (2.8 to 4.92. Finally, genotype Purser should be retained for farmers and programs of genetic amelioration for its resistance to diseases and agronomical performances.

  1. Therapeutic Effects of Allium sativum and Allium cepa in Schistosoma mansoni experimental infection

    Directory of Open Access Journals (Sweden)

    Mona Mohamed Mantawy

    2011-06-01

    Full Text Available The effects of both garlic (Allium sativum and onion (Allium cepa on some biochemical parameters in Schistosoma mansoni infected mice individually and mixed either with or without the currently used drug, praziquantel (PZQ were investigated. These involved some immunological parameters, namely IgM, IgG, interleukins 2 and 6 (IL-2 and 6 and tumor necrosis factor (TNF-α, some antioxidant enzymes [catalase, superoxide dismutase (SOD and glutathione peroxidase (GPX]. In addition, parasitological and histopathological investigations were performed. No changes were observed in the normal control mice treated with dry extract of onion or garlic, individually or mixed, with or without PZQ, compared to the normal healthy control group. Infection with S. mansoni showed an increase in IgG, IgM, IL-2, IL-6, TNF-α and catalase enzyme, accompanied with a decrease in GPX and SOD antioxidant enzyme activities. Remarkable amelioration was noticed in the levels of all the measured parameters in S. mansoni infected mice after administration of the studied extracts. Moreover a significant reduction in worm burden, hepatic and intestinal eggs and oogram count was noticed which was reflected in normalization of liver architecture.

  2. Mining, characterization and validation of EST derived microsatellites from the transcriptome database of Allium sativum L

    Science.gov (United States)

    Chand, Subodh Kumar; Nanda, Satyabrata; Rout, Ellojita; Joshi, Raj Kumar

    2015-01-01

    Expressed Sequence Tags (ESTs) with comprehensive transcript information are valuable resources for development of molecular markers as they are derived from conserved genic regions. The present study highlights the mining of EST database to deduce the class I hyper variable SSRs in A. sativum. From 21694 garlic EST sequences, 642 non-redundant SSRs were identified with an average frequency of 1 per 14.9 kb of garlic transcriptome. The most abundant SSR motifs were the mononucleotides (32.86%) followed by trinucleotides (28.50%) and dinucleotides (13.39%). Among the individual SSRs, (A/T)n accounted for the highest number (137; 21.33%) followed by (G/C)n (74; 11.52%) and (AAG)n (63;9.81%). Primers designed from a robust set of 7 AsESTSSRs resulted in the amplification of 63 polymorphic alleles in 14 accessions of garlic. The resolving power of the markers varied from 4.286 (AsSSR7) to 18.143 (AsSSR13) while the average marker index (MI) was 5.087. These EST-SSRs markers for garlic could be useful for the improvement of garlic linkage map and could be used for evaluating genetic variation and comparative genomics studies in Allium species. PMID:25987765

  3. Effect of Pisum sativum as protein supplement on buffalo milk production

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    C. Grassi

    2010-02-01

    Full Text Available A study was carried out at an organic buffalo dairy farm in order to investigate the effect of feeding protein peas (Pisum sativum L. as an alternative protein source for buffalo cow diets. Two concentrates were formulated to contain (as fed basis either 350 g/kg of soybean cake (SC or 450 g/kg of peas (PC as the main protein sources. The two concentrates were formulated to be almost isonitrogenous (on average crude protein 240 g/kg DM. Two groups of 10 buffalo cows were used in a 100-day lactation study (from 10 days in milk onwards. Cows were blocked into two groups according to lactation number and previous milk yield and were assigned to one of two dietary treatments: control group was offered in the milking parlour 3 kg of SC, while treatment group was offered the same quantity of PC. All cows were fed a total mixed ration containing 3 kg of SC. Daily milk yield was not affected by treatment, as well as, milk fat and protein percentages, somatic cell count, urea content and fatty acid composition.

  4. Effect of salinity and ascorbic acid on growth, water and osmotic relations of Lepidium sativum

    Directory of Open Access Journals (Sweden)

    Azadeh Najjar-Khodabakhsh

    2015-06-01

    Full Text Available Soil salinity is a serious environmental problem in arid and semi-arid regions that have negative impacts on crop production. In this research, because of medicinal and nutritional importance of the garden cress (Lepidium sativum plant, the interactive effects of salinity (225 mM NaCl and ascorbic acid (1 mM were evaluated on growth and its water relations. A completely randomized design with four replications was conducted under controlled conditions. The results showed that salinity decreased growth, relative water content, osmotic potential and soluble proteins and increased soluble sugars, amino acids and proline contents. Many of salinity damaged characteristic were improved by adding exogenous ascorbic acid to salty environment. Exogenous ascorbic acid alone, in comparison with control, enhanced the growth of garden cress by increasing relative water content, soluble sugars and soluble proteins contents. The results indicated that usage of ascorbic acid, as an antioxidant, reduced harmful effects of salinity stress and led to growth improvement in garden cress plants.

  5. Pre-fractionation strategies to resolve pea (Pisum sativum) sub-proteomes

    Science.gov (United States)

    Meisrimler, Claudia-Nicole; Menckhoff, Ljiljana; Kukavica, Biljana M.; Lüthje, Sabine

    2015-01-01

    Legumes are important crop plants and pea (Pisum sativum L.) has been investigated as a model with respect to several physiological aspects. The sequencing of the pea genome has not been completed. Therefore, proteomic approaches are currently limited. Nevertheless, the increasing numbers of available EST-databases as well as the high homology of the pea and medicago genome (Medicago truncatula Gaertner) allow the successful identification of proteins. Due to the un-sequenced pea genome, pre-fractionation approaches have been used in pea proteomic surveys in the past. Aside from a number of selective proteome studies on crude extracts and the chloroplast, few studies have targeted other components such as the pea secretome, an important sub-proteome of interest due to its role in abiotic and biotic stress processes. The secretome itself can be further divided into different sub-proteomes (plasma membrane, apoplast, cell wall proteins). Cell fractionation in combination with different gel-electrophoresis, chromatography methods and protein identification by mass spectrometry are important partners to gain insight into pea sub-proteomes, post-translational modifications and protein functions. Overall, pea proteomics needs to link numerous existing physiological and biochemical data to gain further insight into adaptation processes, which play important roles in field applications. Future developments and directions in pea proteomics are discussed. PMID:26539198

  6. Purification and Characterization of a Lectin from Green Split Peas (Pisum sativum).

    Science.gov (United States)

    Ng, Tzi Bun; Chan, Yau Sang; Ng, Charlene Cheuk Wing; Wong, Jack Ho

    2015-11-01

    Lectins have captured the attention of a large number of researchers on account of their various exploitable activities, including antitumor, immunomodulatory, antifungal, as well as HIV reverse transcriptase inhibitory activities. A mannose/glucose-specific lectin was isolated from green split peas (a variety of Pisum sativum) and characterized. The purification step involved anion-exchange chromatography on a DEAE-cellulose column, cation-exchange chromatography on an SP-Sepharose column, and gel filtration by fast protein liquid chromatography (FPLC) on Superdex 200. The purified lectin had a native molecular mass of around 50 kDa as determined by size exclusion chromatography. It appeared as a heterotetramer, composed of two distinct polypeptide bands with a molecular mass of 6 and 19 kDa, respectively, in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The N-terminal sequence of green split pea lectin shows some degree of homology compared to lectins from other legume species. Its hemagglutinating activity was inhibited by glucose, mannose, and sucrose, and attenuated at pH values higher than 12 or lower than 3. Hemagglutinating activity was preserved at temperatures lower than 80 °C. The lectin did not show antifungal activity toward fungi including Fusarium oxysporum, Botrytis cinerea, and Mycosphaerella arachidicola. Green split pea lectin showed a mitogenic effect toward murine splenocytes and could inhibit the activity of HIV-1 reverse transcriptase.

  7. Studies on antioxidative enzymes induced by cadmium in pea plants (Pisum sativum).

    Science.gov (United States)

    Pandey, Nalini; Singh, Gaurav Kumar

    2012-03-01

    Pea plants (Pisum sativum cv. Swati) exposed to different concentration of cadmium (50,100, 200 microM Cd) under controlled glass house conditions were quantified for different physiological parameters and antioxidative enzymes. In pea plants, Cd produced a significant inhibition of growth and induced chlorosis, marginal yellowing and necrosis in young leaves, the effect being most pronounced at 200 microM Cd supply. An alteration in the activated oxygen metabolism of pea plants were also detected as evidenced by an increase in concentration of H2O2 and TBARS along with decrease in the chlorophyll and carotenoid concentration in leaves. Cadmium toxicity induced an increase in non-protein thiol, ascorbate, proline and cysteine concentration. A significant increment in the activity of SOD, APX and GR, and a decrease in CAT was observed as a result of Cd treatment. The enhanced activity of SOD and inhibition of CAT and POD produces a high build up of H2O2 which appears to be the main cause of oxidative stress due to Cd toxicity in pea plants.

  8. Evaluation of genetic divergence and heritability in pea (Pisum sativum L.

    Directory of Open Access Journals (Sweden)

    Natalia Georgieva

    2016-04-01

    Full Text Available An experiment on genetic evaluation of five genotypes of forage pea (Glyans, Svit, Kamerton, Modus, Pleven 4 was conducted during 2012-2014 period. Analysis of variance showed significant differences among genotypes for the traits pod width, seeds per plant, seed weight per plant and 1000 seed weight. The estimates of genetic parameters of five varieties of Pisum sativum L. indicated a good amount of genetic variation in the experimental materials under investigation. Moderate phenotypic and genotypic coefficients of variation were observed for most of traits except pod length and pod width. For the traits studied seeds per plant, seed weight per plant and plant height were found high heritability along with high genetic gain indicating preponderance of additive effects. Therefore, selection programme based on these characters would be more effective in improving yield parameters of forage pea. The seed yield was positively and significantly correlated with 1000 seed weight and pod stem, which suggested the possibilities of improving seed yield by simultaneous improvement of these traits.

  9. Changes in free polyamines and related enzymes during stipule and pod wall development in Pisum sativum.

    Science.gov (United States)

    Chattopadhyay, Soumen; Lahiri, Kajari; Bharati, Ghosh

    2002-08-01

    Level of free polyamines, their key metabolic enzymes, and other features related to ageing were examined during stipule and pod wall development in pea (Pisum sativum). Free polyamine titre (per unit fresh mass) in both the organs, the specific activities of arginine decarboxylase and ornithine decarboxylase in the pod wall, gradually decreased with maturation. In stipule, these enzymes attained peak activity at 15 days after pod emergence and declined thereafter. Ornithine decarboxylase activity was greater in pod wall than in stipule; while, arginine decarboxylase activity was higher in stipule. Activity of degradative enzyme diamine oxidase increased with the onset of senescence in both the organs. Chlorophyll and electrical conductance had a inverse relationship throughout the experimental period, whereas, the chlorophyll content was directly related with polyamine levels in both stipule and pod wall during aging. On the other hand, protein and RNA contents were positively correlated with free polyamines throughout the test period in stipule, but in the pod wall this was true only for the later stages of development.

  10. Isozymes of beta-N-Acetylhexosaminidase from Pea Seeds (Pisum sativum L.).

    Science.gov (United States)

    Harley, S M; Beevers, L

    1987-12-01

    Four isozymes of beta-N-acetylhexosaminidase (beta-NAHA) from pea seeds (Pisum sativum L.) have been separated, with one, designated beta-NAHA-II, purified to apparent homogeneity by means of an affinity column constructed by ligating p-aminophenyl-N-acetyl-beta-d-thioglucosaminide to Affi-Gel 202. The other three isozymes have been separated and purified 500- to 1750-fold by chromatography on Concanavalin A-Sepharose, Zn(2+) charged immobilized metal affinity chromatography, hydrophobic chromatography, and ion exchange chromatography on CM-Sephadex. All four isozymes are located in the protein bodies of the cotyledons. The molecular weight of each isozyme is 210,000. beta-NAHA-II is composed of two heterogenous subunits. The subunits are not held together by disulfide bonds, but sulfhydryl groups are important for catalysis. All four isozymes release p-nitrophenol from both p-nitrophenyl-N-acetyl-beta-d-glucosaminide and p-nitrophenyl-N-acetyl-beta-d-galactosaminide. The ratio of activity for hydrolysis of the two substrates is pH dependent. The K(m) value for the two substrates and pH optima of the isozymes are comparable to beta-NAHAs from other plant sources.

  11. Pb2+ exposure induced microsatellite instability in Pisum sativum in a locus related with glutamine metabolism.

    Science.gov (United States)

    Rodriguez, E; Azevedo, R; Moreira, H; Souto, L; Santos, Conceição

    2013-01-01

    Lead (Pb) is a toxic element, but its putative mutagenic effects in plant cells, using molecular markers, remain to unveil. To evaluate if Pb induces mutagenicity, Pisum sativum L. seedlings were exposed to Pb(2+) (up to 2000 mg L(-1)) for 28 days and the instability of microsatellites (or Simple Sequence Repeats, SSR) was analyzed in leaves and roots. The analysis of eight selected microsatellites (SSR1-SSR8) demonstrated that only at the highest dosage microsatellite instability (MSI) occurred, at a frequency of 4.2%. Changes were detected in one microsatellite (SSR6) that is inserted in the locus for glutamine synthetase. SSR6 products of roots exposed to the highest concentration of Pb were 3 bp larger than those of the control. Our data demonstrate that: (a) SSR technique is sensitive to detect Pb-induced mutagenicity in plants. MSI instability is Pb dose dependent and organ dependent (roots are more sensitive); (b) the Pb-sensitive SSR6 is inserted in the glutamine synthetase locus, with still unknown relation with functional changes of this enzyme; (c) Pb levels inducing MSI are much above the maximum admitted levels in some European Union countries for agricultural purpose waters. In conclusion, we propose here the potential use of SSR to evaluate Pb(2+)-induced mutagenicity, in combination with other genetic markers.

  12. Development and Characterization of 37 Novel EST-SSR Markers in Pisum sativum (Fabaceae

    Directory of Open Access Journals (Sweden)

    Xiaofeng Zhuang

    2013-01-01

    Full Text Available Premise of the study: Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea linkage maps. In particular, the SSR markers were developed to assist in mapping of white mold disease resistance quantitative trait loci. Methods and Results: Primer pairs were designed for 46 SSRs identified in EST contiguous sequences assembled from a 454 pyrosequenced transcriptome of the pea cultivar, ‘LIFTER’. Thirty-seven SSR markers amplified PCR products, of which 11 (30% SSR markers produced polymorphism in 23 individuals, including parents of recombinant inbred lines, with two to four alleles. The observed and expected heterozygosities ranged from 0 to 0.43 and from 0.31 to 0.83, respectively. Conclusions: These EST-SSR markers for pea will be useful for refinement of pea linkage maps, and will likely be useful for comparative mapping of pea and as tools for marker-based pea breeding.

  13. De Novo Assembly of the Pea (Pisum sativum L. Nodule Transcriptome

    Directory of Open Access Journals (Sweden)

    Vladimir A. Zhukov

    2015-01-01

    Full Text Available The large size and complexity of the garden pea (Pisum sativum L. genome hamper its sequencing and the discovery of pea gene resources. Although transcriptome sequencing provides extensive information about expressed genes, some tissue-specific transcripts can only be identified from particular organs under appropriate conditions. In this study, we performed RNA sequencing of polyadenylated transcripts from young pea nodules and root tips on an Illumina GAIIx system, followed by de novo transcriptome assembly using the Trinity program. We obtained more than 58,000 and 37,000 contigs from “Nodules” and “Root Tips” assemblies, respectively. The quality of the assemblies was assessed by comparison with pea expressed sequence tags and transcriptome sequencing project data available from NCBI website. The “Nodules” assembly was compared with the “Root Tips” assembly and with pea transcriptome sequencing data from projects indicating tissue specificity. As a result, approximately 13,000 nodule-specific contigs were found and annotated by alignment to known plant protein-coding sequences and by Gene Ontology searching. Of these, 581 sequences were found to possess full CDSs and could thus be considered as novel nodule-specific transcripts of pea. The information about pea nodule-specific gene sequences can be applied for gene-based markers creation, polymorphism studies, and real-time PCR.

  14. ALCOHOLIC TINCTURE OF GARLIC (Allium sativum ON GASTROINTESTINAL ENDOPARASITES OF SHEEP- SHORT COMMUNICATION

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    Fernanda Carlini Cunha Santos

    2014-03-01

    Full Text Available The continuous and indiscriminate use of anthelmintic drugs has caused a growing problem of parasite resistance to conventional treatments. In this context, plant essential oils and active components can be used as alternatives or in association with current anthel¬mintic treatment. Garlic (Allium sativum is a herbal medicine with various therapeutic properties: immunostimulating, anticancer, hepatoprotective, antioxidant, antiviral, antifungal and antiparasitic. The aim of this experiment was to evaluate the effect of garlic alcoholic tincture on gastrointestinal endoparasites of sheep. For this, 54 sheep were randomly divided into three treatments. Treatment 1 (T1 received 40 g of garlic alcoholic tincture orally; treatment 2 (T2 received 60 g orally; T3 was the control group. Fecal samples were collected at days 0, 7 and 14, to perform laboratorial tests. Reduction of eggs per gram of feces in T1 and T2 was 0% on days 7 and 14. No difference in larvae genus was identified between treatments or days. In this experiment garlic alcoholic tincture presented no effect on gastrointestinal endoparasites of sheep.

  15. Pre-fractionation strategies to resolve pea (Pisum sativum sub-proteomes

    Directory of Open Access Journals (Sweden)

    Claudia Nicole Meisrimler

    2015-10-01

    Full Text Available Legumes are important crop plants and pea (Pisum sativum L. has been investigated as a model with respect to several physiological aspects. The sequencing of the pea genome has not been completed. Therefore, proteomic approaches are currently limited. Nevertheless, the increasing numbers of available EST-databases as well as the high homology of the pea and medicago genome (Medicago truncatula G. allow the successful identification of proteins. Due to the un-sequenced pea genome, pre-fractionation approaches have been used in pea proteomic surveys in the past. Aside from a number of selective proteome studies on crude extracts and the chloroplast, few studies have targeted other components such as the pea secretome, an important sub-proteome of interest due to its role in abiotic and biotic stress processes. The secretome itself can be further divided into different sub-proteomes (plasma membrane, apoplast, cell wall proteins. Cell fractionation in combination with different gel-electrophoresis, chromatography methods and protein identification by mass spectrometry are important partners to gain insight into pea sub-proteomes, post-translational modifications and protein functions. Overall, pea proteomics needs to link numerous existing physiological and biochemical data to gain further insight into adaptation processes, which play important roles in field applications. Future developments and directions in pea proteomics are discussed.

  16. Role of LLD, a new locus for leaflet/pinna morphogenesis in Pisum sativum

    Indian Academy of Sciences (India)

    Seema Prajapati; Sushil Kumar

    2001-12-01

    Properties of a mutant at the LLD (LEAF-LET DEVELOPMENT) locus in pea Pisum sativum L. are reported in this paper. Plants homozygous for the Mendelian recessive mutation lld bear leaves in which a few to many leaflets are incompletely developed. Opposite pinnae of rachis nodes often formed fused incompletely developed leaflets. The lld mutation was observed to abort pinna development at almost all morphogenetic stages. The lld mutation demonstrated high penetrance and low expressivity. The phenotypes of lld plants in tl, tac, tl tac, tl af and tl af tac backgrounds suggested that LLD function is involved in the separation of lateral adjacent blastozones differentiated on primary, secondary and tertiary rachides and lamina development in leaflets. The aborted development of tendrils and leaflets in lld mutants was related to deficiency in vascular tissue growth. The morphological and anatomical features of the leaflets formed on a tl lld double mutant permitted a model of basipetal leaflet development. The key steps of leaflet morphogenesis include origin of the lamina by splitting of a radially symmetrical growing pinna having abaxial outer surface, opposite to the vascular cylinder, through an invaginational groove, differentiation of adaxial surface along the outer boundary of split tissue in the groove and expansion of the lamina ridges so formed into lamina spans.

  17. Immunocytochemical localization of Pisum sativum TRXs f and m in non-photosynthetic tissues.

    Science.gov (United States)

    Traverso, José A; Vignols, Florence; Cazalis, Roland; Serrato, Antonio J; Pulido, Pablo; Sahrawy, Mariam; Meyer, Yves; Cejudo, Francisco Javier; Chueca, Ana

    2008-01-01

    Plants are the organisms containing the most complex multigenic family for thioredoxins (TRX). Several types of TRXs are targeted to chloroplasts, which have been classified into four subgroups: m, f, x, and y. Among them, TRXs f and m were the first plastidial TRXs characterized, and their function as redox modulators of enzymes involved in carbon assimilation in the chloroplast has been well-established. Both TRXs, f and m, were named according to their ability to reduce plastidial fructose-1,6-bisphosphatase (FBPase) and malate dehydrogenase (MDH), respectively. Evidence is presented here based on the immunocytochemistry of the localization of f and m-type TRXs from Pisum sativum in non-photosynthetic tissues. Both TRXs showed a different spatial pattern. Whilst PsTRXm was localized to vascular tissues of all the organs analysed (leaves, stems, and roots), PsTRXf was localized to more specific cells next to xylem vessels and vascular cambium. Heterologous complementation analysis of the yeast mutant EMY63, deficient in both yeast TRXs, by the pea plastidial TRXs suggests that PsTRXm, but not PsTRXf, is involved in the mechanism of reactive oxygen species (ROS) detoxification. In agreement with this function, the PsTRXm gene was induced in roots of pea plants in response to hydrogen peroxide.

  18. Bacteriological evaluation of Allium sativum oil as a new medicament for pulpotomy of primary teeth

    Science.gov (United States)

    Mohammad, Shukry Gamal; Baroudi, Kusai

    2015-01-01

    Objective: To compare the effects of Allium sativum oil and formocresol on the pulp tissue of the pulpotomized teeth. Materials and Methods: Twenty children were selected for this study. All children had a pair of non-vital primary molars. A sterile paper point was dipped in the root canals prior to the mortal pulpotomy. These paper points were collected in transfer media and immediately transported to the microbiological lab to be investigated microbiologically (for Streptococcus mutans and Lactobacillus acidophilus). Then the procedure of mortal pulpotomy was performed. After 2 weeks, the cotton pellets were removed and sterile paper points were dipped in the root canals for microbiological examination. Then comparison between the count of bacteria before and after treatment was conducted. Statistical analysis was performed using independent t-test and paired t-test at the significance level of α = 0.05. Results: After application of both medicaments, there was a marked decrease in S. mutans and L. acidophilus counts. The difference between the mean of log values of the count before and after the application was highly significant for both medicaments (P formocresol on the bacteria of the infected root canals. PMID:25992338

  19. EFEITO DA COBERTURA MORTA NO CRESCIMENTO E NA PRODUÇÃO DO ALHO (Allium sativum L. THE EFFECT OF THE MULCHING IN THE GROWTH AND BULB PRODUCTION OF GARLIC (Allium sativum L.

    Directory of Open Access Journals (Sweden)

    Jácomo Divino Borges

    2007-09-01

    Full Text Available

    Foi avaliado o efeito das coberturas mortas do solo: palha de arroz, casca de arroz, cepilho de madeira, grama cortada e folhas de árvores, no crescimento e na produção do alho (Allium sativum L. cultivar Cateto Roxo. Os tratamentos de cobertura do solo foram feitos logo após o plantio e em camada suficiente apenas para cobrir o solo. Concluiu-se que a cobertura morta favoreceu o crescimento inicial e a produção de bulbos. Os materiais testados em cobertura do solo mostraram-se equivalentes, porém com tendência para o tratamento com casca de arroz superar os demais.

    PALAVRAS-CHAVE: Alho; AIlium sativum; cobertura morta dos solos; produção.

    The effect of flue mulching in the growth and bulb production of garlic (Allium sativum L. cv. ‘Cateto Roxo’, was evaluated. The soil cover treatments: rice straw, rice husk, smoothing plane, cut grass and dry leaves of tree, was done just after planting and in layers just to cover the soil. It was concluded that mulching favoured initial growing and bulb production. Tested materials were showed to be equivalent however with tendency to rice husk overcome the other ones.

    KEY-WORDS: Garlic; production; mulching; growth.

  20. Característica higroscópica e termodinâmica do coentro desidratado Hygroscopic and thermodynamic features of dehydrated coriander

    Directory of Open Access Journals (Sweden)

    Adriano Sant'Ana Silva

    2010-06-01

    analysis, development of case study of food and moisture-solid interactions. Thus, this work aimed to study the behavior of adsorption isotherms of leaves and stems of dehydrate coriander, evaluate the model that represents the behavior of the curves and determine the isosteric heat of adsorption. The experimental data were fitted by nonlinear regression, the GAB, Oswin, Smith, BET, Halsey and Peleg models had been used. The models which best represented the behavior of the adsorption curves of the leaf and stem were from Smith and Peleg. The isosteric heat of adsorption obtained from dried leaf is larger than the stem.

  1. Diversity of Micromonospora strains isolated from nitrogen fixing nodules and rhizosphere of Pisum sativum analyzed by multilocus sequence analysis.

    Science.gov (United States)

    Carro, Lorena; Spröer, Cathrin; Alonso, Pilar; Trujillo, Martha E

    2012-03-01

    It was recently reported that Micromonospora inhabits the intracellular tissues of nitrogen fixing nodules of the wild legume Lupinus angustifolius. To determine if Micromonospora populations are also present in nitrogen fixing nodules of cultivated legumes such as Pisum sativum, we carried out the isolation of this actinobacterium from P. sativum plants collected in two man-managed fields in the region of Castilla and León (Spain). In this work, we describe the isolation of 93 Micromonospora strains recovered from nitrogen fixing nodules and the rhizosphere of P. sativum. The genomic diversity of the strains was analyzed by amplified ribosomal DNA restriction analysis (ARDRA). Forty-six isolates and 34 reference strains were further analyzed using a multilocus sequence analysis scheme developed to address the phylogeny of the genus Micromonospora and to evaluate the species distribution in the two studied habitats. The MLSA results were evaluated by DNA-DNA hybridization to determine their usefulness for the delineation of Micromonospora at the species level. In most cases, DDH values below 70% were obtained with strains that shared a sequence similarity of 98.5% or less. Thus, MLSA studies clearly supported the established taxonomy of the genus Micromonospora and indicated that genomic species could be delineated as groups of strains that share > 98.5% sequence similarity based on the 5 genes selected. The species diversity of the strains isolated from both the rhizosphere and nodules was very high and in many cases the new strains could not be related to any of the currently described species.

  2. Faba bean (Vicia faba minor) and pea seeds (Pisum sativum) as protein sources in lactating ewes’ diets.

    OpenAIRE

    D. Gatta; M.L. Martini; Casini, L.; G.B. Liponi

    2010-01-01

    18 Massese lactating ewes, divided into 3 homogeneous groups for parity and milk yield, were used to evaluate the replacement effects of soybean meal by Faba bean (Vicia faba minor) and Pea (Pisum sativum) seeds. During a 70 days trial (beginning after weaning: 30±1.5 days after lambing) animals were fed three isonitrogenous and isocaloric diets. Each diet was characterised by the presence of only one protein feed. The diets consisted of alfalfa hay (1.1 kg/head/d) and a decreasing amo...

  3. THE EFFECT OF INDUSTRIAL WATER ON THE GERMINATION OF SEEDS AND THE DEVELOPMENT OF SEEDLINGS OF PISUM SATIVUM L.

    OpenAIRE

    Liliana Cristina SOARE; Codruţa-Mihaela DOBRESCU; Liana Elena LASCU

    2011-01-01

    The aim of the research was to highlight the influence of industrial water on the germination of seeds and the development of seedlings of Pisum sativum L., so that the impact of these water on the plants may be assessed, in case of accidental spills. The analysis of polluted industrial water indicates the presence of Cr, Fe, Ni, Pb, Cd, Cu, and Zn concentrations higher than CMA, except Cd, which was below the detection limit, and Cu. On leaving the neutralization station, metal concentration...

  4. Fumigant toxicity of plant essential oils against Camptomyia corticalis (Diptera: Cecidomyiidae).

    Science.gov (United States)

    Kim, Jun-Ran; Haribalan, Perumalsamy; Son, Bong-Ki; Ahn, Young-Joon

    2012-08-01

    The toxicity of 98 plant essential oils against third instars of cecidomyiid gall midge Camptomyia corticalis (Loew) (Diptera: Cecidomyiidae) was examined using a vapor-phase mortality bioassay. Results were compared with that of a conventional insecticide dichlorvos. Based on 24-h LC50 values, all essential oils were less toxic than dichlorvos (LC50, 0.027 mg/cm3). The LC50 of caraway (Carum carvi L.) seed, armoise (Artemisia vulgaris L.), clary sage (Salvia sclarea L.), oregano (Origanum vulgare L.), lemongrass [Cymbopogon citratus (DC.) Stapf], niaouli (Melaleuca viridiflora Gaertner), spearmint (Mentha spicata L.), cassia especial (Cinnamomum cassia Nees ex Blume), Dalmatian sage (Salvia offcinalis L.), red thyme (Thymus vulgaris L.), bay [Pimenta racemosa (P. Mill.) J.W. Moore], garlic (Allium sativum L.), and pennyroyal (Mentha pulegium L.) oils is between 0.55 and 0.60 mg/cm3. The LC50 of cassia (C. cassia, pure and redistilled), white thyme (T. vulgaris), star anise (Illicium verum Hook.f.), peppermint (Mentha X piperita L.), wintergreen (Gaultheria procumbens L.), cinnamon (Cinnamomum zeylanicum Blume) bark, sweet marjoram (Origanum majorana L.), Roman chamomile [Chamaemelum nobile (L.) All.], eucalyptus (Eucalyptus globulus Labill.), rosemary (Rosmarinus officinalis L.),Virginian cedarwood (Juniperus virginiana L.), pimento berry [Pimenta dioica (L.) Merr.], summer savory (Satureja hortensis L.), lavender (Lavandula angustifolia Mill.), and coriander (Coriandrum sativum L.) oils is between 0.61 and 0.99 mg/cm3. All other essential oils tested exhibited low toxicity to the cecidomyiid larvae (LC50, >0.99 mg/cm3). Global efforts to reduce the level of highly toxic synthetic insecticides in the agricultural environment justify further studies on the active essential oils as potential larvicides for the control of C. corticalis populations as fumigants with contact action.

  5. Mutagénesis inducida en microbulbos de Allium sativum L.

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    Adriana Pardo Roldán

    2015-07-01

    Full Text Available Se estableció un protocolo de mutagénesis en microbulbos de ajo (Allium sativum L. clon Boconó cultivado in vitro. Para el efecto se realizaron dos ensayos, uno de radiosensibilidad para establecer la dosimetría apropiada de radiación gamma y otro de mutagénesis para determinar el comportamiento de los materiales hasta la etapa de almacenamiento. En el primero los microbulbos fueron tratados con cuatros dosis de radiación gamma (6, 8, 10 y 12 Krad, más un control. Para establecer la dosis óptima se consideró la sobrevivencia del 50% de los microbulbos (DL50. Se empleó un diseño de bloques al azar con cinco tratamientos y 20 repeticiones por tratamiento. En el ensayo mutagénico los microbulbos fueron irradiados con 8 y 10 Krad y almacenados durante 45 días a 10 °C en condiciones de oscuridad En este caso se utilizó un diseño de bloques al azar con tres tratamientos (0, 8 y 10 Krad y 20 repeticiones por tratamiento. En ambos ensayos, los microbulbos irradiados con 8 y 10 Krad registraron los mayores promedios para peso y diámetro, lo cual permite concluir que estas dosis son adecuadas para favorecer la producción de mutantes con características agronómicas deseables en el clon Boconó

  6. Inhibitory activity of garlic (Allium sativum extract on multidrug-resistant Streptococcus mutans

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    Fani M

    2007-01-01

    Full Text Available Garlic ( Allium sativum extract has been known to have inhibitory activity on various pathogenic bacteria, viruses and fungi. The objective of present investigation was to study in vitro inhibitory activity of garlic extract on multidrug-resistant (MDR strains of Streptococcus mutans isolated from human carious teeth. Filter sterilized aqueous extract of garlic was prepared and used in the present study. For isolation of S. mutans , extracted human carious teeth were cultured in Todd-Hewit broth and Mitis-Salivarius-Bacitracin agar. S. mutans was characterized by colony morphology, biochemical tests and other conventional bacteriological procedures. Disk sensitivity tests and broth dilution methods were used to determine antibiotic sensitivity profile and inhibitory activity of garlic extract on S. mutans isolated from carious teeth. Of 105 carious teeth tested, 92 (87.6% isolates of S. mutans were recovered, among which 28 (30.4% were MDR since they were resistant to four or more antibiotics. The highest rate of resistance was observed for tetracycline (30.4% and least resistance (0% to teichoplanin and vancomycin while 22.8% and 23.9% of the isolates were resistant to penicillin and amoxicillin, respectively. Chlorhexidine minimum inhibitory concentration (MIC for MDR and non-MDR S. mutans varied from 2 to 16 µg ml−1 and from 0.25 to 1 µg ml−1 , respectively ( P < 0.05. All isolates, MDR and non-MDR of S. mutans were sensitive to garlic extract with the MIC ranging from 4 to 32 mg ml−1 . Considering in vitro data obtained in the present study, mouthwashes or toothpaste containing optimum concentration of garlic extract could be used for prevention of dental caries.

  7. Differing mechanisms of simple nitrile formation on glucosinolate degradation in Lepidium sativum and Nasturtium officinale seeds.

    Science.gov (United States)

    Williams, David J; Critchley, Christa; Pun, Sharon; Chaliha, Mridusmita; O'Hare, Timothy J

    2009-01-01

    Glucosinolates are sulphur-containing glycosides found in brassicaceous plants that can be hydrolysed enzymatically by plant myrosinase or non-enzymatically to form primarily isothiocyanates and/or simple nitriles. From a human health perspective, isothiocyanates are quite important because they are major inducers of carcinogen-detoxifying enzymes. Two of the most potent inducers are benzyl isothiocyanate (BITC) present in garden cress (Lepidium sativum), and phenylethyl isothiocyanate (PEITC) present in watercress (Nasturtium officinale). Previous studies on these salad crops have indicated that significant amounts of simple nitriles are produced at the expense of the isothiocyanates. These studies also suggested that nitrile formation may occur by different pathways: (1) under the control of specifier protein in garden cress and (2) by an unspecified, non-enzymatic path in watercress. In an effort to understand more about the mechanisms involved in simple nitrile formation in these species, we analysed their seeds for specifier protein and myrosinase activities, endogenous iron content and glucosinolate degradation products after addition of different iron species, specific chelators and various heat treatments. We confirmed that simple nitrile formation was predominantly under specifier protein control (thiocyanate-forming protein) in garden cress seeds. Limited thermal degradation of the major glucosinolate, glucotropaeolin (benzyl glucosinolate), occurred when seed material was heated to >120 degrees C. In the watercress seeds, however, we show for the first time that gluconasturtiin (phenylethyl glucosinolate) undergoes a non-enzymatic, iron-dependent degradation to a simple nitrile. On heating the seeds to 120 degrees C or greater, thermal degradation of this heat-labile glucosinolate increased simple nitrile levels many fold.

  8. Long-Term Fungal Inhibition by Pisum sativum Flour Hydrolysate during Storage of Wheat Flour Bread

    Science.gov (United States)

    Lavecchia, Anna; Gramaglia, Valerio; Gobbetti, Marco

    2015-01-01

    In order to identify antifungal compounds from natural sources to be used as ingredients in the bakery industry, water/salt-soluble extracts (WSE) from different legume flour hydrolysates obtained by the use of a fungal protease were assayed against Penicillium roqueforti DPPMAF1. The agar diffusion assays allowed the selection of the pea (Pisum sativum) hydrolysate as the most active. As shown by the hyphal radial growth rate, the WSE had inhibitory activity towards several fungi isolated from bakeries. The MIC of the WSE was 9.0 mg/ml. Fungal inhibition was slightly affected by heating and variations in pH. The antifungal activity was attributed to three native proteins (pea defensins 1 and 2 and a nonspecific lipid transfer protein [nsLTP]) and a mixture of peptides released during hydrolysis. The three proteins have been reported previously as components of the defense system of the plant. Five peptides were purified from WSE and were identified as sequences encrypted in leginsulin A, vicilin, provicilin, and the nsLTP. To confirm antifungal activity, the peptides were chemically synthesized and tested. Freeze-dried WSE were used as ingredients in leavened baked goods. In particular, breads made by the addition of 1.6% (wt/wt) of the extract and fermented by baker's yeast or sourdough were characterized for their main chemical, structural, and sensory features, packed in polyethylene bags, stored at room temperature, and compared to controls prepared without pea hydrolysate. Artificially inoculated slices of a bread containing the WSE did not show contamination by fungi until at least 21 days of storage and behaved like the bread prepared with calcium propionate (0.3%, wt/wt). PMID:25862230

  9. Preservative potential of cumin essential oil for Pisum sativum L. during storage

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    Kumar Narendra

    2016-04-01

    Full Text Available The samples of stored seeds of pea (Pisum sativum L. were collected from 30 farmer markets. The mycobiota analysis showed presence of 15 fungal species and one species of insect Callosobruchus chinensis. The fungi such as Aspergillus flavus, A. niger, A. ochraceous, A. terreus were found to be dominant based on percent frequency of each in blotter method in unsterilized and sterilized seeds 18.9-7.9, 15.0-3.9, 12.2-3.7, 10.1-1.7, respectively, and in agar plate technique 17.9-8.3, 15.1-9.5, 12.8-5, 7.9.7-6.7, respectively. These species showed reduction in terms of weight loss, germination and protein content in pathogenicity testing. Essential oil extracted by hydrodistillation from fruits of Cuminum cyminum L. was evaluated against the most common occurring funi such as A. flavus and A. niger as well as the insect species C. chinensis and the oil exhibited high toxicity. The oil killed the tested fungi and showed thermostable nature at its minimum inhibitory concentration (MIC of 400 ppm. The oil safely preserved pea seeds up to 120 days at 0.50 (1,000 ppm and 0.76 ml (1,500 ppm in polyethylene and jute bags of 500 ml capacity containing 400 g seeds separately. There were no changes in organoleptic appereance of food seeds during storage. The oil has beneficial effect on number of visible nodule formation and shoot and root dry biomass of 15-day-old plants in comparison to control sets. The cumin oil was analyzed by gas chromatography (GC and gas chromatography-mass spectrometry (GC-MS.

  10. Stimulation of nodulation in field peas (Pisum sativum) by low concentrations of ammonium in hydroponic culture

    Science.gov (United States)

    Waterer, J. G.; Vessey, J. K.; Raper, C. D. Jr; Raper CD, J. r. (Principal Investigator)

    1992-01-01

    Although the inhibitory effects of high concentrations of mineral N (> 1.0 mM) on nodule development and function have often been studied, the effects of low, static concentrations of NH4+ (hydroponic culture on nodule establishment and nitrogenase activity in field peas [Pisum sativum L. cv. Express (Svalof AB)] for the initial 28 days after planting (DAP). Peas grown in the presence of low concentrations of NH4+ had significantly greater nodule numbers (up to 4-fold) than plants grown without NH4+. Nodule dry weight per plant was significantly higher at 14, 21 and 28 DAP in plants grown in the presence of NH4+, but individual nodule mass was lower than in plants grown without NH4+. The nodulation pattern of the plants supplied with NH4+ was similar to that often reported for supernodulating mutants, however the plants did not express other growth habits associated with supernodulation. Estimates of N2 fixation indicate that the plus-NH4+ peas fixed as much or more N2 than the plants supplied with minus-NH4+ nutrient solution. There were no significant differences in nodule numbers, nodule mass or NH4+ uptake between the plants grown at the two concentrations of NH4+. Nodulation appeared to autoregulate by 14 DAP in the minus-NH4+ treatment. Plant growth and N accumulation in the minus-NH4+ plants lagged behind those of the plus-NH4+ treatments prior to N2 fixation becoming well established in the final week of the experiment. The plus-NH4+ treatments appeared not to elicit autoregulation and plants continued to initiate nodules throughout the experiment.

  11. Safety evaluation studies on Garden cress (Lepidium sativum L. seeds in Wistar rats

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    P K Datta

    2011-03-01

    Full Text Available Summary: Garden cress (Lepidium sativum L.  leaves and seeds are used in India as  food supplement and also in traditional medicine. We have assessed the safety of Garden cress (GC seeds by conducting acute and subchronic toxicity studies in adult Wistar rats. For the acute toxicity study, 0.5 –5.0 g/kg body weight of the GC seed powder was administered through diet to rats and obvious symptoms of toxicity and mortality were monitored for 72 h . Acute doses of GC seed powder did not induce any symptoms of toxicity or mortality of  rats. In subchronic toxicity study, 1.0 – 10.0% of the GC powder was administered to rats through diet for 14 weeks. Dietary feeding of GC seed powder did not produce any mortality, no significant changes in food intake, gain in body weight, relative weight of  organs, hematological parameters, macroscopic and microscopic changes in vital organs, were observed between  experimental and control groups.  Clinical enzymes viz., LDH, SGPT were within normal levels, however, the serum ALP and SGOT were significantly increased in male rats receiving 5.0 and 10 % of GC seeds. The results showed that acute and subchronic feeding of GC seed for 14 weeks did not produce any toxic effects in male and female rats and thus can be considered non-toxic and safe.   Industrial relevance: Herbal medicines are very popular and extensively used in the developing countries. Garden cress seeds and leaves are used in food preparations.  GC seeds are given to pregnant and lactating women as natural food supplement to increase milk secretion.  GC seeds are used in herbal based medicinal preparations. The data on the acute and subchronic toxicity studies on medicinal plants are essential to assess its safety to humans, particularly for its use in pharmaceutical preparations.

  12. Repellent activities of dichloromethane extract of Allium sativum (garlic (Liliaceae against Hyalomma rufipes (Acari

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    Felix Nchu

    2016-02-01

    Full Text Available Dichloromethane (DCM extract of garlic (Allium sativum Linn. bulbs was assessed for its repellent effect against the hard tick, Hyalomma rufipes (Acari: Ixodidae using two tick behavioural bioassays; Type A and Type B repellency bioassays, under laboratory conditions. These bioassays exploit the questing behaviour of H. rufipes, a tick that in nature displays ambush strategy, seeking its host by climbing up on vegetation and attaching to a passing host. One hundred microlitres (100 µL of the test solution containing DCM extract of garlic bulbs and DCM at concentrations of 0.35%, 0.7% or 1.4% w/v were evaluated. DCM only was used for control. Tick repellency increased significantly (R2 = 0.98 with increasing concentration (40.03% – 86.96% yielding an EC50 of 0.45% w/v in Type B repellency bioassay. At concentration of 1.4% w/v, the DCM extract of garlic bulbs produced high repellency index of 87% (male ticks and 87.5% (female ticks in the Type A repellency bioassay. Only 4% avoidance of male ticks or female ticks was recorded in the Type B repellency bioassay. In the corresponding controls, the mean numbers of non-repelled male or female ticks were 80% and 41 males or 38 females of 50 ticks in the Type A and Type B repellency bioassays, respectively. The variations in the results could be attributed to the difference in tick repellent behaviours that were assessed by the two repellency bioassays; the Type A repellency bioassay assessed repellent effect of garlic extracts without discriminating between deterrence and avoidance whereas the Type B repellency bioassay only assessed avoidance response. Generally, DCM extract of garlic was repellent against H. rufipes, albeit weak tick repellency was obtained in the Type B repellency bioassay. Furthermore, this study established that the tick repellent activity of garlic extracts is predominantly by deterrence.

  13. Potency of Allium sativum and Allium cepa Oils against Schistosoma mansoni Infection in Mice

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    Nadia S. Metwally

    2006-06-01

    Full Text Available Introduction: It has been reported that garlic (Allium sativum and onion (Allium cepa are used all over the world in different diseases, such as infections, injuries, gastrointestinal dysfunctions and cardiovascular diseases. Therefore, our aim in this work was to study the ability of garlic and onion oils to offset the infectivity as well as the metabolic disturbances induced by Schistosoma mansoni parasitism. Methods: The two current drugs were given in a dosage of 5ml / kg body weight/ day. Three aspects of drug action were investigated, the effect on S. mansoni infection, the effect on liver functions, and on liver metabolism. The parasitological investigation included worm burden and ova count. Results: Serum biochemical analysis of infected mice revealed a significant increase in the levels of aspartate aminotransferase (AST, alanine aminotransferase (ALT , ­ glutamyltransferase (GGT , alkaline phosphatase ( ALP, acid phosphatase (AP, while a decrease in glucose, total lipids total cholesterol, high - and low- density lipoproteins cholesterol (HDL and LDL, triglycerides, total proteins and albumin was observed. Liver tissue analysis of infected animals showed a marked increase in L- hydroxyproline (HP concentration and xanthine oxidase (XO activity accompanied with a reduction in total adenosinetriphosphatase (ATPase and phosphofructokinase (PFK enzymatic activities. Treatment with either garlic or onion oils greatly normalized liver function enzymes and variably improved the other parameters with a noticeable reduction in worm burden and ova count. Conclusions: It could be concluded that garlic or onion may play a role against the metabolic disturbances caused by S. mansoni infection, owing to an effect which may be induced by improving the immunological host system and their antioxidant activities

  14. Effects of long-term reduced tillage on weed infestation of pea (Pisum sativum L.

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    Andrzej Woźniak

    2014-09-01

    Full Text Available The study evaluated weed infestation of pea (Pisum sativum L. cultivated under conditions of conventional (CT, reduced (RT and herbicide tillage (HT. It demonstrated the highest weed density per m2 in plots with the herbicide (HT and reduced (RT systems and significantly lower weed infestation in plots cultivated in the conventional system (CT. In addition, more weeds occurred at the third leaf stage (13/14 in BBCH scale than at the pod development stage (73/74 BBCH of pea. The highest biomass was produced by weeds in the herbicide system (HT, a lower one – in the reduced system (RT, and the lowest one – in the conventional system (CT. The air-dry weight of weeds depended also on pea development stage. At the pod development stage (73/74 BBCH, the air-dry weight of weeds was significantly higher than at the third leaf stage (13/14 BBCH. The tillage system was also observed to influence the species composition of weeds. This trait was also affected by the period of weed infestation assessment. At the third leaf stage of pea (13/14 BBCH, there occurred 26 weed species, including 24 annual ones. The most abundant species included: Chenopodium album L., Stellaria media (L. Vill., Capsella bursa-pastoris (L. Med., Matricaria inodora L., Thlaspi arvense L., and Fallopia convolvulus (L. A. Löve. At the pod development stage (73/74 BBCH, the pea crop was colonized by 24 weed species, including 3 perennial ones. At this stage the predominant species included: Avena fatua L., Amaranthus retroflexus L., Papaver rhoeas L., Echinochloa crus-galli (L. P.B., Matricaria inodora L., and Galeopsis tetrahit L.

  15. Bean pod mottle virus: a new powerful tool for functional genomics studies in Pisum sativum.

    Science.gov (United States)

    Meziadi, Chouaib; Blanchet, Sophie; Richard, Manon M S; Pilet-Nayel, Marie-Laure; Geffroy, Valérie; Pflieger, Stéphanie

    2016-08-01

    Pea (Pisum sativum L.) is an important legume worldwide. The importance of pea in arable rotations and nutritional value for both human and animal consumption have fostered sustained production and different studies to improve agronomic traits of interest. Moreover, complete sequencing of the pea genome is currently underway and will lead to the identification of a large number of genes potentially associated with important agronomic traits. Because stable genetic transformation is laborious for pea, virus-induced gene silencing (VIGS) appears as a powerful alternative technology for determining the function of unknown genes. In this work, we present a rapid and efficient viral inoculation method using DNA infectious plasmids of Bean pod mottle virus (BPMV)-derived VIGS vector. Six pea genotypes with important genes controlling biotic and/or abiotic stresses were found susceptible to BPMV carrying a GFP reporter gene and showed fluorescence in both shoots and roots. In a second step, we investigated 37 additional pea genotypes and found that 30 were susceptible to BPMV and only 7 were resistant. The capacity of BPMV to induce silencing of endogenes was investigated in the most susceptible genotype using two visual reporter genes: PsPDS and PsKORRIGAN1 (PsKOR1) encoding PHYTOENE DESATURASE and a 1,4-β-D-glucanase, respectively. The features of the 'one-step' BPMV-derived VIGS vector include (i) the ease of rub-inoculation, without any need for biolistic or agro-inoculation procedures, (ii) simple cost-effective procedure and (iii) noninterference of viral symptoms with silencing. These features make BPMV the most adapted VIGS vector in pea to make low- to high-throughput VIGS studies.

  16. Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L

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    Ayeh Kwadwo

    2011-11-01

    Full Text Available Abstract Background The def mutant pea (Pisum sativum L showed non-abscission of seeds from the funicule. Here we present data on seed development and growth pattern and their relationship in predicting this particular trait in wild type and mutant lines as well as the inheritance pattern of the def allele in F2 and F3 populations. Findings Pod length and seed fresh weight increase with fruit maturity and this may affect the abscission event in pea seeds. However, the seed position in either the distal and proximal ends of the pod did not show any difference. The growth factors of seed fresh weight (FW, width of funicles (WFN, seed width (SW and seed height (SH were highly correlated and their relationships were determined in both wild type and def mutant peas. The coefficient of determination R2 values for the relationship between WFN and FW, SW and SH and their various interactions were higher for the def dwarf type. Stepwise multiple regression analysis showed that variation of WFN was associated with SH and SW. Pearson's chi square analysis revealed that the inheritance and segregation of the Def locus in 3:1 ratio was significant in two F2 populations. Structural analysis of the F3 population was used to confirm the inheritance status of the Def locus in F2 heterozygote plants. Conclusions This study investigated the inheritance of the presence or absence of the Def allele, controlling the presence of an abscission zone (AZ or an abscission-less zone (ALZ forming in wild type and mutant lines respectively. The single major gene (Def controlling this phenotype was monogenic and def mutants were characterized and controlled by the homozygous recessive def allele that showed no palisade layers in the hilum region of the seed coat.

  17. Stimulation of nodulation in field peas (Pisum sativum) by low concentrations of ammonium in hydroponic culture

    Science.gov (United States)

    Waterer, J. G.; Vessey, J. K.; Raper, C. D. Jr; Raper CD, J. r. (Principal Investigator)

    1992-01-01

    Although the inhibitory effects of high concentrations of mineral N (> 1.0 mM) on nodule development and function have often been studied, the effects of low, static concentrations of NH4+ (nodulation are unknown. In the present experiments we examine the effects of static concentrations of NH4+ at 0, 0.1 and 0.5 mM in flowing, hydroponic culture on nodule establishment and nitrogenase activity in field peas [Pisum sativum L. cv. Express (Svalof AB)] for the initial 28 days after planting (DAP). Peas grown in the presence of low concentrations of NH4+ had significantly greater nodule numbers (up to 4-fold) than plants grown without NH4+. Nodule dry weight per plant was significantly higher at 14, 21 and 28 DAP in plants grown in the presence of NH4+, but individual nodule mass was lower than in plants grown without NH4+. The nodulation pattern of the plants supplied with NH4+ was similar to that often reported for supernodulating mutants, however the plants did not express other growth habits associated with supernodulation. Estimates of N2 fixation indicate that the plus-NH4+ peas fixed as much or more N2 than the plants supplied with minus-NH4+ nutrient solution. There were no significant differences in nodule numbers, nodule mass or NH4+ uptake between the plants grown at the two concentrations of NH4+. Nodulation appeared to autoregulate by 14 DAP in the minus-NH4+ treatment. Plant growth and N accumulation in the minus-NH4+ plants lagged behind those of the plus-NH4+ treatments prior to N2 fixation becoming well established in the final week of the experiment. The plus-NH4+ treatments appeared not to elicit autoregulation and plants continued to initiate nodules throughout the experiment.

  18. Repellent activities of dichloromethane extract of Allium sativum (garlic) (Liliaceae) against Hyalomma rufipes (Acari).

    Science.gov (United States)

    Nchu, Felix; Magano, Solomon R; Eloff, Jacobus N

    2016-12-02

    Dichloromethane (DCM) extract of garlic (Allium sativum Linn.) bulbs was assessed for its repellent effect against the hard tick, Hyalomma rufipes (Acari: Ixodidae) using two tick behavioural bioassays; Type A and Type B repellency bioassays, under laboratory conditions. These bioassays exploit the questing behaviour of H. rufipes, a tick that in nature displays ambush strategy, seeking its host by climbing up on vegetation and attaching to a passing host. One hundred microlitres (100 µL) of the test solution containing DCM extract of garlic bulbs and DCM at concentrations of 0.35%, 0.7% or 1.4% w/v were evaluated. DCM only was used for control. Tick repellency increased significantly (R2 = 0.98) with increasing concentration (40.03% - 86.96%) yielding an EC50 of 0.45% w/v in Type B repellency bioassay. At concentration of 1.4% w/v, the DCM extract of garlic bulbs produced high repellency index of 87% (male ticks) and 87.5% (female ticks) in the Type A repellency bioassay. Only 4% avoidance of male ticks or female ticks was recorded in the Type B repellency bioassay. In the corresponding controls, the mean numbers of non-repelled male or female ticks were 80% and 41 males or 38 females of 50 ticks in the Type A and Type B repellency bioassays, respectively. The variations in the results could be attributed to the difference in tick repellent behaviours that were assessed by the two repellency bioassays; the Type A repellency bioassay assessed repellent effect of garlic extracts without discriminating between deterrence and avoidance whereas the Type B repellency bioassay only assessed avoidance response. Generally, DCM extract of garlic was repellent against H. rufipes, albeit weak tick repellency was obtained in the Type B repellency bioassay. Furthermore, this study established that the tick repellent activity of garlic extracts is predominantly by deterrence.

  19. The tropic response of plant roots to oxygen: oxytropism in Pisum sativum L

    Science.gov (United States)

    Porterfield, D. M.; Musgrave, M. E.

    1998-01-01

    Plant roots are known to orient growth through the soil by gravitropism, hydrotropism, and thigmotropism. Recent observations of plant roots that developed in a microgravity environment in space suggested that plant roots may also orient their growth toward oxygen (oxytropism). Using garden pea (Pisum sativum L. cv. Weibul's Apollo) and an agravitropic mutant (cv. Ageotropum), root oxytropism was studied in the controlled environment of a microrhizotron. A series of channels in the microrhizotron allowed establishment of an oxygen gradient of 0.8 mmol mol-1 mm-1. Curvature of seedling roots was determined prior to freezing the roots for subsequent spectrophotometric determinations of alcohol dehydrogenase activity. Oxytropic curvature was observed all along the gradient in both cultivars of pea. The normal gravitropic cultivar showed a maximal curvature of 45 degrees after 48 h, while the agravitropic mutant curved to 90 degrees. In each cultivar, the amount of curvature declined as the oxygen concentration decreased, and was linearly related to the root elongation rate. Since oxytropic curvature occurred in roots exposed to oxygen concentrations that were not low enough to induce the hypoxically responsive protein alcohol dehydrogenase, we suspect that the oxygen sensor associated with oxytropism does not control the induction of hypoxic metabolism. Our results indicate that oxygen can play a critical role in determining root orientation as well as impacting root metabolic status. Oxytropism allows roots to avoid oxygen-deprived soil strata and may also be the basis of an auto-avoidance mechanism, decreasing the competition between roots for water and nutrients as well as oxygen.

  20. Antihypertensive properties of Allium sativum (garlic) on normotensive and two kidney one clip hypertensive rats.

    Science.gov (United States)

    Nwokocha, C R; Ozolua, R I; Owu, D U; Nwokocha, M I; Ugwu, A C

    2011-12-20

    Allium sativum (garlic) is reported to act as an antihypertensive amidst an inconsistency of evidence. In this study, we investigated the cardiovascular effects of aqueous garlic extracts (AGE) on normotensive and hypertensive rats using the two-kidney one-clip (2K1C) model. Mean arterial blood pressure (MAP) and heart rate (HR) were measured in normotensive and 2K1C rat models anesthetized with thiopentone sodium (50 mg/kg body weight i.p.) through the left common carotid artery connected to a recording apparatus. The jugular vein was cannulated for administration of drugs. Intravenous injection of AGE (5-20 mg/kg) caused a significant (p<0.05) decrease in both MAP and HR in a dose-dependent manner in both the normotensive and 2K1C models, with more effects on normotensive than 2K1C rat model. The dose of 20mg/kg of AGE significantly (p<0.05) reduced systolic (16.7 ± 2.0%), diastolic (26.7 ± 5.2%), MAP (23.1 ± 3.6%) and HR (38.4 ± 4.3%) in normotensive rats. In 2K1C group, it significantly reduced systolic (22.2 ± 2.1 %), diastolic (30.6 ± 3.2%), MAP (28.2 ± 3.1%) and HR (45.2 ± 3.5%) from basal levels. Pulse pressure was significantly elevated (33.3 ±5.1%) in the 2K1C group. Pretreatment of the animals with muscarinic receptor antagonist, atropine (2 mg/kg, i.v.), did not affect the hypotensive and the negative chronotropic activities of the extract. AGE caused a decrease in blood pressure and bradycardia by direct mechanism not involving the cholinergic pathway in both normotensive and 2K1C rats, suggesting a likely involvement of peripheral mechanism for hypotension.

  1. Effect of Rhizobium inoculation of seeds and foliar fertilization on productivity of Pisum sativum L.

    Directory of Open Access Journals (Sweden)

    Tadeusz Zając

    2013-07-01

    Full Text Available Pea (Pisum sativum L. is the second most important grain legume crop in the world which has a wide array of uses for human food and fodder. One of the major factors that determines the use of field pea is the yield potential of cultivars. Presently, pre-sowing inoculation of pea seeds and foliar application of microelement fertilizers are prospective solutions and may be reasonable agrotechnical options. This research was undertaken because of the potentially high productivity of the 'afila' morphotype in good wheat complex soils. The aim of the study was to determine the effect of vaccination with Rhizobium and foliar micronutrient fertilization on yield of the afila pea variety. The research was based on a two-year (2009–2010 controlled field experiment, conducted in four replicates and carried out on the experimental field of the Bayer company located in Modzurów, Silesian region. experimental field soil was Umbrisol – slightly degraded chernozem, formed from loess. Nitragina inoculant, as a source of symbiotic bacteria, was applied before sowing seeds. Green area index (GAI of the canopy, photosynthetically active radiation (PAR, and normalized difference vegetation index (NDVI were determined at characteristic growth stages. The presented results of this study on symbiotic nitrogen fixation by leguminous plants show that the combined application of Nitragina and Photrel was the best combination for productivity. Remote measurements of the pea canopy indexes indicated the formation of the optimum leaf area which effectively used photosynthetically active radiation. The use of Nitragina as a donor of effective Rhizobium for pea plants resulted in slightly higher GAI values and the optimization of PAR and NDVI. It is not recommended to use foliar fertilizers or Nitragina separately due to the slowing of pea productivity.

  2. Radiosensitivity study in the germination and growth of the pea Pisum sativum L, with seeds exposed to gamma radiation; Estudio de radiosensibilidad en la germinacion y crecimiento de la arveja Pisum sativum L, con semillas expuestas a radiacion gamma

    Energy Technology Data Exchange (ETDEWEB)

    Ilguan, J.; Carrasco, J.; Marquez, V., E-mail: ilguanjanneth@gmail.com [Escuela Superior Politecnica de Chimborazo, Facultad de Ciencias, Panamericana Sur Km 1 1/2, ECO60155 Riobamba (Ecuador)

    2016-10-15

    Seeds of Pisum sativum L. were irradiated in a {sup 60}Co irradiator belonging to the Subsecretaria de Control y Aplicaciones Nucleares del Ecuador (SCAN), the dose rate at the irradiation time was 4.86 Gy/min. The seeds were grouped in packs of 100 units and exposed at doses of 20, 40, 60, 80, 100, 120, 140, 160, 180 and 200 Gy. The number of effective germinations, the days for germination, the main stem thickness, the number of branches and the size of the plant were evaluated. To validate the experimentation, the data were analyzed statistically by tests of homogeneity of variances, way ANOVA and Scheffe analysis to each variable. The number of effective germinations was given to 100% for 20 and 40 Gy then decreases to a higher dose reaching 20% to 200 Gy, being the average of the proof near to 90%. Increased germination stimulation at treatment at 20 Gy with an average of 5.0 days was evidenced. Treatment at doses of 20, 40 and 60 Gy generate a plant size greater than the proof whose mean is 68.30 cm. The mean number of branches of the proof is 5.3 with a standard deviation of 0.675, which is greater at doses of 20 and 40 Gy. In the study, is concluded that better results are obtained when irradiating seeds Pisum sativum L, between 20 and 60 Gy. (Author)

  3. [Effect of several extracts derived from plants on the survival of larvae of Aedes fluviatilis (Lutz) (Diptera: Culicidae) in the laboratory].

    Science.gov (United States)

    Consoli, R A; Mendes, N M; Pereira, J P; Santos, B de S; Lamounier, M A

    1988-01-01

    The larvicidal properties of 34 plant extracts were tested against Aedes fluviatilis (Lutz) (Diptera: Culicidae) larvae, at 100, 10 and 1 ppm concentrations; 26.6% of the extracts enhanced larval mortality (alpha = 0.05) at 100 ppm (Anacardium occidentale, Agave americana, Allium sativum, Coriandrum sativum, Nerium oleander, Spatodea campanulata, Tibouchina scrobiculata and Vernonia salzmanni). Anacardic acid (A. occidentale) was effective at 10 ppm and A. sativum (crude extract) at 1 ppm.

  4. Determination of the Water Requirements of Garlic (Alium Sativum L. and Its Relationship With the Crop's Development

    Directory of Open Access Journals (Sweden)

    Hugo Eduardo Castro Franco

    2014-11-01

    Full Text Available Despite the importance of garlic (Allium sativum L., there is not enough information available about the water requirements for garlic crop in the country. The present study is to identify the crop water requirements for each phenological stage and set a watering schedule according to environmental conditions offered in Tunja-Boyacá. This research was conducted during the first half of 2013, on the farm called “La Maria", which is located in the Pedagogical and Technological University of Colombia. The assessment of the phenological stages in garlic was developed through a stratified sampling design and a destructive sampling design, which were carried out every 7 days after transplant. The value of Kc was determined through the FAO-56 methodology, in which the method was used to find the crop evapotranspiration. In order to establish crop water use, two lysimeters of 1 m3 of capacity were installed. The result of ETo was obtained through the weather station data, these data were analyzed with the Penman-Monteith equation, using the Cropwat software. Three phenological stages for growing garlic (Allium sativum L. were established, from the transplant to the harvest. These stages were: vegetative Growth and development, Bulb initiation and Maturation. Kc values for each phenological stage were 0.95, 0.97 and 0.68 respectively.

  5. Therapeutic Effects of Allium sativum on Lead-induced Biochemical changes in Soft tissues of Swiss Albino Mice

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    Arti Sharma

    2009-01-01

    Full Text Available Allium sativum (Meaning pungent belongs to the Alliaceae family and genus Allium, is generally known in the developing world for its characteristic flavor, a medicinal plant and a source of vegetable oil. Besides, the plant is reported to have various biological activities including hypocholesterolemic, antiatherosclerotic, anticoagulant, antibacterial, antifungal, anti-diabetic, anti-tumor agent; used for treating various disease such as inflammation, cardiovascular and liver diseases. The objective of this study is to investigate the therapeutic effects of Allium sativum on lead induced toxicity in mice. Chronic dose of lead (2 mg/Kg body weight, i.p., showed significant decrease in antioxidant enzymes such as superoxide dismutase (SOD, catalase (CAT and the nonenzymatic antioxidant as glutathione (GSH and total protein content in the liver, kidney and brain. This decrease was accompanied with significant increase in lipid peroxidation and cholesterol level. Also, there were disturbances in the liver, kidney and brain functions manifested by significant changes in their functional markers. Efficacy of garlic to reduce tissue lead concentration was also evaluated. Mostly, all of the investigated parameters were restored nearly to the normal values after raw garlic extract treatment. In conclusion, garlic exerts its effects not only as an antioxidant but also as a sulfur donor. So, garlic has a promising role and it is worth to be considered as a natural chelating agent for lead intoxication.

  6. Alkali-Soluble Pectin Is the Primary Target of Aluminum Immobilization in Root Border Cells of Pea (Pisum sativum)

    Science.gov (United States)

    Yang, Jin; Qu, Mei; Fang, Jing; Shen, Ren Fang; Feng, Ying Ming; Liu, Jia You; Bian, Jian Feng; Wu, Li Shu; He, Yong Ming; Yu, Min

    2016-01-01

    We investigated the hypothesis that a discrepancy of Al binding in cell wall constituents determines Al mobility in root border cells (RBCs) of pea (Pisum sativum), which provides protection for RBCs and root apices under Al toxicity. Plants of pea (P. sativum L. ‘Zhongwan no. 6’) were subjected to Al treatments under mist culture. The concentration of Al in RBCs was much higher than that in the root apex. The Al content in RBCs surrounding one root apex (104 RBCs) was approximately 24.5% of the total Al in the root apex (0–2.5 mm), indicating a shielding role of RBCs for the root apex under Al toxicity. Cell wall analysis showed that Al accumulated predominantly in alkali-soluble pectin (pectin 2) of RBCs. This could be attributed to a significant increase of uronic acids under Al toxicity, higher capacity of Al adsorption in pectin 2 [5.3-fold higher than that of chelate-soluble pectin (pectin 1)], and lower ratio of Al desorption from pectin 2 (8.5%) compared with pectin 1 (68.5%). These results indicate that pectin 2 is the primary target of Al immobilization in RBCs of pea, which impairs Al access to the intracellular space of RBCs and mobility to root apices, and therefore protects root apices and RBCs from Al toxicity. PMID:27679639

  7. Molecular detection and in vitro antioxidant activity of S-allyl-L-cysteine (SAC) extracted from Allium sativum.

    Science.gov (United States)

    Sun, Y-E; Wang, W-D

    2016-06-30

    It is well known that Allium sativum has potential applications to clinical treatment of various cancers due to its remarkable ability in eliminating free radicals and increasing metabolism. An allyl-substituted cysteine derivative - S-allyl-L-cysteine (SAC) was separated and identified from Allium sativum. The extracted SAC was reacted with 1-pyrenemethanol to obtain pyrene-labelled SAC (Py-SAC) to give SAC fluorescence properties. Molecular detection of Py-SAC was conducted by steady-state fluorescence spectroscopy and time-resolved fluorescence method to quantitatively measure concentrations of Py-SAC solutions. The ability of removing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical using Py-SAC was determined through oxygen radical absorbance capacity (ORAC). Results showed the activity of Py-SAC and Vitamin C (VC) with ORAC as index, the concentrations of Py-SAC and VC were 58.43 mg/L and 5.72 mg/L respectively to scavenge DPPH, and 8.16 mg/L and 1.67 mg/L to scavenge •OH respectively. Compared with VC, the clearance rates of Py-SAC to scavenge DPPH were much higher, Py-SAC could inhibit hydroxyl radical. The ability of removing radical showed a dose-dependent relationship within the scope of the drug concentration.

  8. Alkali-Soluble Pectin Is the Primary Target of Aluminum Immobilization in Root Border Cells of Pea (Pisum sativum).

    Science.gov (United States)

    Yang, Jin; Qu, Mei; Fang, Jing; Shen, Ren Fang; Feng, Ying Ming; Liu, Jia You; Bian, Jian Feng; Wu, Li Shu; He, Yong Ming; Yu, Min

    2016-01-01

    We investigated the hypothesis that a discrepancy of Al binding in cell wall constituents determines Al mobility in root border cells (RBCs) of pea (Pisum sativum), which provides protection for RBCs and root apices under Al toxicity. Plants of pea (P. sativum L. 'Zhongwan no. 6') were subjected to Al treatments under mist culture. The concentration of Al in RBCs was much higher than that in the root apex. The Al content in RBCs surrounding one root apex (10(4) RBCs) was approximately 24.5% of the total Al in the root apex (0-2.5 mm), indicating a shielding role of RBCs for the root apex under Al toxicity. Cell wall analysis showed that Al accumulated predominantly in alkali-soluble pectin (pectin 2) of RBCs. This could be attributed to a significant increase of uronic acids under Al toxicity, higher capacity of Al adsorption in pectin 2 [5.3-fold higher than that of chelate-soluble pectin (pectin 1)], and lower ratio of Al desorption from pectin 2 (8.5%) compared with pectin 1 (68.5%). These results indicate that pectin 2 is the primary target of Al immobilization in RBCs of pea, which impairs Al access to the intracellular space of RBCs and mobility to root apices, and therefore protects root apices and RBCs from Al toxicity.

  9. The effect of the humic acid and garlic (Allium sativum L. on performance parameters and carcass characteristic of broiler chicken

    Directory of Open Access Journals (Sweden)

    Veronika PISTOVÁ

    2016-12-01

    Full Text Available In this study the effect of humic acids and humic acids and garlic powder (Allium sativum L. combination on performance parameters and carcass characteristic of broiler chickens were studied. A total of 120 Ross 308 broiler chickens were divided into 3 treatments (n=40. The control group of chickens was fed complete feed mixtures without any additives. Treatment T1 was fed complete feed mixtures containing 1% of humic acid. Treatment T2 was fed complete feed mixtures containing 1.8% of humic acid and 0.2% of garlic powder (Allium sativum L.. At the end of the experiment was average body weight (values in the order of the groups: 1796.4±188.1; 1999.1±355.8 and 1958.6±201.2 g±SD significantly higher (P≤0.05 in both treatments groups compared to control group. In T1 was weight of gizzard (values in the order of the groups: 34.9±5.2; 43.1±9.4 and 38.9±7.0 g±SD significantly higher (P≤0.05 compared to control group. Carcass weight, weight of heart weight of liver and carcass yield of treatments groups was higher, but differences in these indicators were not statistically significant (P≥0.05. The results of this experiment indicate that humic acids may by an alternative replacement of feed antibiotics.

  10. Proteomic Profiling of the Microsomal Root Fraction: Discrimination of Pisum sativum L. Cultivars and Identification of Putative Root Growth Markers

    Science.gov (United States)

    Meisrimler, Claudia-Nicole; Wienkoop, Stefanie; Lüthje, Sabine

    2017-01-01

    Legumes are a large and economically important family, containing a variety of crop plants. Alongside different cereals, some fruits, and tropical roots, a number of leguminosae evolved for millennia as crops with human society. One of these legumes is Pisum sativum L., the common garden pea. In the past, breeding has been largely selective on improved above-ground organs. However, parameters, such as root-growth, which determines acquisition of nutrients and water, have largely been underestimated. Although the genome of P. sativum is still not fully sequenced, multiple proteomic studies have been published on a variety of physiological aspects in the last years. The presented work focused on the connection between root length and the influence of the microsomal root proteome of four different pea cultivars after five days of germination (cultivar Vroege, Girl from the Rhineland, Kelvedon Wonder, and Blauwschokker). In total, 60 proteins were identified to have significantly differential abundances in the four cultivars. Root growth of five-days old seedlings and their microsomal proteome revealed a similar separation pattern, suggesting that cultivar-specific root growth performance is explained by differential membrane and ribosomal protein levels. Hence, we reveal and discuss several putative root growth protein markers possibly playing a key role for improved primary root growth breeding strategies. PMID:28257117

  11. In vitro Free Radical Scavenging Potential of Defatted Ethanolic Extract of the Seeds of Lepidium sativum Linn.

    Institute of Scientific and Technical Information of China (English)

    Annie Shirwaikar; Bharatkumar Patel; Yogesh Kamariya; Vinit Parmar; Saleemulla Khan

    2011-01-01

    AIM:The objective of the present study was to evaluate the antioxidant potential of the defatted ethanolic extract of the seeds of Lepidium Sativum Linn.METHODS:Different in vitro chemical assays viz.DPPH (1,l-diphenyl-2-picrylhydrazyl) radical scavenging,ABTS (2,2-azinobis- (3-ethylbenzothiazoline-6-sulphonate) radical scavenging,iron chelation,lipid peroxidation,superoxide scavenging and non-enzymatic haemoglobin glycosylation assay were used.The total antioxidant capacity of the extract was determined spectrophotometrically by phosphomolybdic acid method.RESULT & CONCLUSION:The defatted lepidium seed extract showed significant free radical scavenging activity in ABTS and non-enzymatic glycosylation assays,and a moderate activity in all the other assays.IC5o of the extract in the DPPH,ABTS,iron chelation,lipid peroxidation and super oxide scavenging assays were found to be 171.13,38.64,128.94,71.39 and 206.09 μg.mL-1 respectively.The haemoglobin glycosylation assay of the extract showed a percentage scavenging of 46.60% and 74.88%,at 0.5 and 1.0 μg.mL-1,concentrations,respectively.Total antioxidant capacity of ethanolic extract of L.sativum (10 mg·mL-1) was found to be equivalent to 58.38 μg.mL-1 of ascorbic acid.

  12. Plant extracts in the control of aphids Brevicoryne brassicae (L. and Myzus persicae (SulzerExtratos vegetais no controle dos afídeos Brevicoryne brassicae (L. e Myzus persicae (Sulzer

    Directory of Open Access Journals (Sweden)

    Rafael Reginato Ávila

    2011-07-01

    Full Text Available Were accomplished the effect of plant extracts of clove basil (Ocimum gratissimum L., horsetail (Equisetum hyemale L., coriander (Coriandrum sativum L. and tobacco (Nicotiana tabacum L. on Brevicoryne brassicae (L., 1758 and Myzus persicae (Sulzer, 1776 aphids in cabbage Brassica oleracea (L.. The treatments consisted of plant extracts prepared fresh and dry (concentrations of 2.5; 5.0; and 10% and the controls insecticide acephate and water. These solutions were sprayed on cabbage discs placed on agar in Petri dishes, containing twenty adult aphids. In sequence, the Petri dishes were sealed with plastic film and this procedure was repeated for the two aphid species studied. The assessment of the number of live nymphs and adults occurred at 1, 12, 24, and 72 hours after installation. The extracts of coriander and tobacco prepared in a concentration of 10% showed toxic effects similar to the organophosphate insecticide acephate, on adults and nymphs of the aphids Brevicoryne brassicae and Myzus persicae. Coriander revealed a promising alternative that deserves detailed studies regarding the performance of its active ingredients and dosage determination in order to provide a safe herbal product to control insects.Avaliou-se o efeito de extratos vegetais de alfavaca-cravo (Ocimum gratissimum L., cavalinha (Equisetum hyemale L., coentro (Coriandrum sativum L. e fumo (Nicotiana tabacum L. sobre os pulgões Brevicoryne brassicae (L., 1758 e Myzus persicae (Sulzer, 1776 em couve Brassica oleracea (L.. Os tratamentos consistiram de extratos vegetais preparados a fresco e seco (nas concentrações de 2,5; 5,0 e 10%, do padrão inseticida acefato e de água. As soluções assim obtidas foram pulverizadas em discos de couve colocados sobre agar em placas de Petri, contendo vinte pulgões adultos. Na sequência, as placas de Petri foram vedadas com filme plástico transparente, sendo este procedimento repetido para as duas espécies de afídeos. A avalia

  13. The development of an efficient cultivar-independent plant regeneration system from callus derived from both apical and non-apical root segments of garlic (Allium sativum L.)

    NARCIS (Netherlands)

    Zheng, S.J.; Henken, G.; Krens, F.A.; Kik, C.

    2003-01-01

    Callus induction and later plant regeneration were studied in four widely grown garlic (Allium sativum L.) cultivars from Europe. Root segments from in vitro plantlets were used as starting material. In addition to cultivar effects, the effects of auxin and cytokinin levels and the position of the s

  14. Grass pea (Lthyrus sativum L.) as pre-plant organic fertilizer for conventionally tilled winter wheat: effects on yield and quality

    Science.gov (United States)

    Sources of organic nitrogen (N) for the southern Great Plains (SGP) - and methods of their use – need testing to find ways to counter the rising cost of N fertilizer. This study investigated the cool-season pulse grass pea (Lathyrus sativum L.) (GP) as a pre-plant N source for continuous, convention...

  15. Kinetics of Ca2+- and ATP-dependent, voltage-controlled anion conductance in the plasma membrane of mesophyll cells of Pisum sativum

    NARCIS (Netherlands)

    Elzenga, J.T.M.; van Volkenburgh, E.

    1997-01-01

    Whole-cell patch-clamp techniques were used to measure anion currents through the plasma membrane of protoplasts of mesophyll cells of expanding pea (Pisum sativum L.) leaves. Voltage-induced changes of the currents could be modelled with single exponential activation and deactivation kinetics. The

  16. In vitro effect of essential oils from Cinnamomum aromaticum, Citrus limon and Allium sativum on two intestinal flagellates of poultry, Tetratrichomonas gallinarum and Histomonas meleagridis

    Directory of Open Access Journals (Sweden)

    Zenner L.

    2003-06-01

    Full Text Available Essential oils may be effective preventive or curative treatments against several flagelated poultry parasites and may become primordial either to organic farms, or as more drugs are bannished. The anti-flagellate activity of essential oils obtained from fresh leaves of Cinnamomum aromaticum, Citrus limon pericarps and Allium sativum bulbs was investigated in vitro on Tetratrichomonas gallinarum and Histomonas meleagridis. On T. gallinarum, the minimal lethal concentration (MLC at 24 hours was 0.25 μl/ml for C. aromaticum oil, and 0.125 μl/ml for C. limon and A. sativum oils. On H. meleagridis, MLC was 0.5 μl/ml for C. aromaticum oil and 1 μl/m l for C. limon and A. sativum oils at 24 and 48 hours. Moreover, no synergistic effects were evidenced in vitro. The essential oil constituents, based on their GC retention times have been also identified. The major component is trans-cinnamaldehyde (79 % for C. aromaticum; limonene for C. limon (71 % and diallyl tri- and disulfide (79 % for A. sativum. Even if concentration and protocol adaptations are required for successful in vivo treatments, it appears that these oils may be useful as chemotherapeutic agents against several poultry parasites.

  17. Plackett-Burman设计和响应面分析法优化香菜中总黄酮的微波提取工艺%Optimization of microwave extraction technologies of flavonoids from Coriandrum sativum L.with Plackett-Burman design and response surface analysis

    Institute of Scientific and Technical Information of China (English)

    刘韬; 李荣; 姜子涛

    2014-01-01

    为了确定香菜茎叶中总黄酮微波辅助提取最佳工艺条件,首先用Plackett-Burman设计对影响香菜总黄酮得率的因素进行评价,筛选出三个主要影响因素:液料比,微波温度,乙醇体积分数.以香菜总黄酮得率为响应值,采用响应面分析法优化提取条件,确定最佳提取条件为液料比35∶1,微波温度80℃,乙醇体积分数67%.在此条件下黄酮得率的预测值为1.33%,实测值为1.31%,误差为1.30%.表明Plackett-Burman设计结合响应面分析法可以很好的对香菜总黄酮微波提取工艺进行优化.

  18. Phytochemical Profiles and Antimicrobial Activities of Allium cepa Red cv. and A. sativum Subjected to Different Drying Methods: A Comparative MS-Based Metabolomics

    Directory of Open Access Journals (Sweden)

    Mohamed A. Farag

    2017-05-01

    Full Text Available Plants of the Allium genus produce sulphur compounds that give them a characteristic (alliaceous flavour and mediate for their medicinal use. In this study, the chemical composition and antimicrobial properties of Allium cepa red cv. and A. sativum in the context of three different drying processes were assessed using metabolomics. Bulbs were dried using either microwave, air drying, or freeze drying and further subjected to chemical analysis of their composition of volatile and non-volatile metabolites. Volatiles were collected using solid phase micro-extraction (SPME coupled to gas chromatography–mass spectrometry (GC/MS with 42 identified volatiles including 30 sulphur compounds, four nitriles, three aromatics, and three esters. Profiling of the polar non-volatile metabolites via ultra-performance liquid chromatography coupled to high resolution MS (UPLC/MS annotated 51 metabolites including dipeptides, flavonoids, phenolic acids, and fatty acids. Major peaks in GC/MS or UPLC/MS contributing to the discrimination between A. sativum and A. cepa red cv. were assigned to sulphur compounds and flavonoids. Whereas sulphur conjugates amounted to the major forms in A. sativum, flavonoids predominated in the chemical composition of A. cepa red cv. With regard to drying impact on Allium metabolites, notable and clear separations among specimens were revealed using principal component analysis (PCA. The PCA scores plot of the UPLC/MS dataset showed closer metabolite composition of microwave dried specimens to freeze dried ones, and distant from air dried bulbs, observed in both A. cepa and A. sativum. Compared to GC/MS, the UPLC/MS derived PCA model was more consistent and better in assessing the impact of drying on Allium metabolism. A phthalate derivative was found exclusively in a commercial garlic preparation via GC/MS, of yet unknown origin. The freeze dried samples of both Allium species exhibited stronger antimicrobial activities compared to

  19. Microscopia electrônica de inclusões citoplas máticas e alterações celulares associadas ao vírus do mosaico da cenoura Electron microscopy of cytoplasmic inclusion and cell modification associated with carrot mosaic virus

    Directory of Open Access Journals (Sweden)

    I. J. B. Camargo

    1971-01-01

    Full Text Available Exames electrono-microscópicos de secções ultrafinas de tecidos foliares de cenoura (Daucus carota L. e coentro (Coriandrum sativumL. infetados pelo vírus do mosaico da cenoura (VMC revelaram a ocorrência de inclusões em forma de bandas densas no citoplasma. Exames mais detalhados dessas bandas revelaram serem elas constituídas pela justaposição de lamelas delgadas (10-15m¼ de espessura, as quais parecem eventualmente desprender-se e formar outros tipos de inclusão, como as de perfil circular e em forma de catavento, comumente associadas à infecção provocada por vírus do grupo Y da batata. Ocasionalmente, partículas do VMC foram observadas nas proximidades dessas inclusões. As dimensões das bandas densas atingiram até 0,l-0,2¼ x 3-4¼, visíveis mesmo em exames ao microscópio convencional. Além da ocorrência dessas inclusões, pôde também ser observada a presença de grande número de vesículas no citoplasma, aparentemente associadas a uma atividade do complexo de Golgi.A virus, inducing mosaic and malformation of carrot leaves (Daucus carota L., was found in Piedade, SP. It was termed carrot mosaic virus (CMV, and is both mechanically and aphid transmitted. Morphologically it belongs to the potato virus Y group (15m¼ x 740m¼. Electron microscopic examination of thin sections of leaf tissues from carrot or coriander (Coriandrum sativum L. infected with CMV demonstrated the presence of cytoplasmic inclusions, usually in the form of dense bands. They could reach sizes up to 0.2¼ x 4¼ and were detectable even under light microscopic examination. These bands, at higher magnification, showed to be built up of thin lamella (10-15m¼ thick, closely apposed. Occasionally individual or group of lamella appeared to loose from the bands, producing other type of profile, such as pin wheels and rings. CMV particles were sometimes observed in the vicinity of these inclusions, often laying parallel to their surface. These

  20. Época de colheita e qualidade fisiológica de sementes de coentro produzidas no norte de Minas Gerais Harvest season and physiological quality of coriander seeds produced in the north of Minas Gerais

    Directory of Open Access Journals (Sweden)

    T.V. Sousa

    2011-01-01

    Full Text Available No presente trabalho, objetivou-se avaliar a época de colheita e a qualidade fisiológica de sementes de coentro produzidas no Norte de Minas Gerais. A primeira época de colheita das sementes foi realizada aos 15 dias após o florescimento pleno, quando aproximadamente 50% das plantas apresentavam flores. As demais colheitas foram realizadas de 7 em 7 dias, até as sementes atingirem 14,0% de umidade, fato que ocorreu na 6ª colheita. Imediatamente após cada colheita, as sementes foram avaliadas quanto ao teor de água, à germinação e ao vigor (testes de primeira contagem, emergência de plântulas e índice de velocidade de emergência. A maturidade fisiológica das sementes de coentro, cultivar Verdão, ocorre entre 42 a 44 dias após o florescimento, quando as sementes apresentam umidade em torno de 28,0%, podendo a colheita ser realizada até 50 dias após o florescimento, quando as sementes apresentavam 14,0% de umidade.The present study aimed to evaluate the harvest season and physiological quality of coriander seeds produced in the North of Minas Gerais. The first season of harvest of the seeds was performed 15 days after full bloom, when approximately 50% of plants had flowers. The other crops were carried out 7 in 7 days, until the seeds reach 14,0% moisture, a fact that occurred in the 6th harvest. Immediately after each harvest, the seeds were evaluated for water content, germination and vigor tests (first count, seedling emergence and emergence speed index. The physiological maturity of coriander seeds, cultivate Verdão, occurs between 42 to 44 days after flowering, when the seeds had humidity around 28,0% at physiological maturity, the harvest may be held until 50 days after flowering, when seeds had 14,0% humidity.