WorldWideScience

Sample records for core complexes revision

  1. Complex coacervate core micelles.

    Science.gov (United States)

    Voets, Ilja K; de Keizer, Arie; Cohen Stuart, Martien A

    2009-01-01

    In this review we present an overview of the literature on the co-assembly of neutral-ionic block, graft, and random copolymers with oppositely charged species in aqueous solution. Oppositely charged species include synthetic (co)polymers of various architectures, biopolymers - such as proteins, enzymes and DNA - multivalent ions, metallic nanoparticles, low molecular weight surfactants, polyelectrolyte block copolymer micelles, metallo-supramolecular polymers, equilibrium polymers, etcetera. The resultant structures are termed complex coacervate core/polyion complex/block ionomer complex/interpolyelectrolyte complex micelles (or vesicles); i.e., in short C3Ms (or C3Vs) and PIC, BIC or IPEC micelles (and vesicles). Formation, structure, dynamics, properties, and function will be discussed. We focus on experimental work; theory and modelling will not be discussed. Recent developments in applications and micelles with heterogeneous coronas are emphasized.

  2. Training Revising Based Traversability Analysis of Complex Terrains for Mobile Robot

    Directory of Open Access Journals (Sweden)

    Rui Song

    2014-05-01

    Full Text Available Traversability analysis is one of the core issues in the autonomous navigation for mobile robots to identify the accessible area by the information of sensors on mobile robots. This paper proposed a model to analyze the traversability of complex terrains based on rough sets and training revising. The model described the traversability for mobile robots by traversability cost. Through the experiment, the paper gets the conclusion that traversability analysis model based on rough sets and training revising can be used where terrain features are rich and complex, can effectively handle the unstructured environment, and can provide reliable and effective decision rules in the autonomous navigation for mobile robots.

  3. Organic complexant topical report. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Meacham, J.E.; and others

    1997-06-26

    This document reviews the current understanding of hazards associated with the storage of organic complexant salts in Hanford Site high-level waste tanks. Two distinct hazards were evaluated: spontaneous self- accelerating decomposition reactions in the bulk material (bulk runaway) and ignition followed by condensed phase propagation (point source ignition). Results from the bulk runaway assessment showed that bulk runaway is not credible for all tanks except C-106. However, speciation of the organic in C-106 shows that it is almost all in the form of low energy oxalate, and there is little potential for a bulk runaway. Additional testing and evaluation would be necessary to definitely conclude that there is no potential for bulk runaway; therefore, controls are currently required for his tank. Temperature monitoring and controls (water addition and active ventilation) are adequate to prevent bulk runaway in C-106.

  4. Seismic analyses of equipment in 2736-Z complex. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Ocoma, E.C.

    1995-04-01

    This report documents the structural qualification for the existing equipment when subjected to seismic loading in the Plutonium Storage Complex. It replaces in entirety Revision 0 and reconciles the U.S. Department of Energy (DOE) comments on Revision 0. The Complex consists of 2736-Z Building (plutonium storage vault), 2736-ZA Building (vault ventilation equipment building), and 2736-ZB Building (shipping/receiving, repackaging activities). The existing equipment structurally qualified in this report are the metal storage racks for 7 inch and lard cans in room 2 of Building 2736-Z; the cubicles, can holders and pedestals in rooms 1, 3, and 4 of Building 2736-Z; the ventilation duct including exhaust fans/motors, emergency diesel generator, and HEPA filter housing in Building 2736-ZA; the repackaging glovebox in Building 2736-ZB; and the interface duct between Buildings 2736-Z and 2736-ZA.

  5. The Core Extrusion Schema-Revised: Hiding Oneself Predicts Severity of Social Interaction Anxiety.

    Science.gov (United States)

    Levinson, Cheri A; Rodebaugh, Thomas L; Lim, Michelle H; Fernandez, Katya C

    2017-01-01

    Cognitive behavioral models of social anxiety disorder (SAD) suggest that fear of negative evaluation is a core fear or vulnerability for SAD. However, why negative evaluation is feared is not fully understood. It is possible that core beliefs contribute to the relationship between fear of negative evaluation and SAD. One of these beliefs may be a core extrusion schema: a constellation of beliefs that one's true self will be rejected by others and therefore one should hide one's true self. In the current study (N = 699), we extended research on the Core Extrusion Schema and created a shortened and revised version of the measure called the Core Extrusion Schema-Revised The Core Extrusion Schema-Revised demonstrated good factor fit for its two subscales (Hidden Self and Rejection of the True Self) and was invariant across gender and ethnicity. The Hidden Self subscale demonstrated excellent incremental validity within the full sample as well as in participants diagnosed with generalized SAD. Specifically, the Hidden Self subscale may help explain severity of social interaction anxiety. This measure could be used with individuals diagnosed with generalized SAD to design exposures targeting these core beliefs.

  6. Analytical chemistry methods for metallic core components: Revision March 1985

    Energy Technology Data Exchange (ETDEWEB)

    1985-03-01

    This standard provides analytical chemistry methods for the analysis of alloys used to fabricate core components. These alloys are 302, 308, 316, 316-Ti, and 321 stainless steels and 600 and 718 Inconels and they may include other 300-series stainless steels.

  7. Thermal history of a metamorphic core complex

    Science.gov (United States)

    Dokka, R. K.; Mahaffie, M. J.; Snoke, A. W.

    1985-01-01

    Fission track (FT) thermochronology studies of lower plate rocks of the Ruby Mountains-East Humbolt Range metamorphic core complex provide important constraints on the timing an nature of major middle Tertiary extension of northeast Nevada. Rocks analyzed include several varieties of mylonitic orthogneiss as well as amphibolitic orthognesses from the non-mylonitic infrastructural core. Oligocene-age porphyritic biotite granodiorite of the Harrison Pass pluton was also studied. The minerals dated include apatite, zircon, and sphene and were obtained from the same rocks that have been previously studied. FT ages are concordant and range in age from 26.4 Ma to 23.8 Ma, with all showing overlap at 1 sigma between 25.4 to 23.4 Ma. Concordancy of all FT ages from all structural levels indicates that the lower plate cooled rapidly from temperatures above approx. 285 C (assumed sphene closure temperature (2)) to below approx. 150 C (assumed apatite closure temperature) near the beginning of the Miocene. This suggests that the lower plate cooled at a rate of at least approx. 36 deg C/Ma during this event. Rapid cooling of the region is considered to reflect large-scale tectonic denudation (intracrustal thinning), the vertical complement to intense crustal extension. FT data firmly establish the upper limit on the timing of mylonitization during detachment faulting and also coincide with the age of extensive landscape disruption.

  8. Cropland Management Plan : Tewaukon National Wildlife Refuge Complex : Revised: April 1, 1996

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document serves as a revision to the Tewaukon National Wildlife Refuge Complex's (TRC) then present cropland management plan. This revision more completely...

  9. A critical revision of the Tubifera ferruginosa complex.

    Science.gov (United States)

    Leontyev, Dmitry V; Schnittler, Martin; Stephenson, Steven L

    2015-01-01

    Based on a combination of morphological and molecular investigations, a critical revision of the widely distributed myxomycete Tubifera ferruginosa is presented. A phylogeny of the morphospecies, based on partial 18S nuc rDNA sequences, displays several clearly distinct clades, all differing by a genetic distance (p distance) of at least 0.15, with the distance within the clades below 0.11. These molecular differences correlate with morphological characters, such as the shape of sporothecal tips, the color of immature fructifications and the ultrastructure of the inner surface of the peridium. The combination of morphological and molecular data provides evidence that T. ferruginosa is actually a species complex, representing at least seven species. These are T. ferruginosa sensu stricto, T. applanata, T. corymbosa, T. dudkae, T. magna, T. montana and T. pseudomicrosperma. Among these T. applanata and T. dudkae (as Reticularia dudkae) were described recently based on morphological characters and the 18S nuc rDNA phylogeny confirmed their separation. Another four species, T. corymbosa, T. magna, T. montana and T. pseudomicrosperma, are described here. We propose an epitype for T. ferruginosa sensu stricto and recognize subsp. ferruginosa and subsp. acutissima within this species. All studied taxa of the T. ferruginosa complex are shown to lack a capillitium. Structures formerly described as capillitium represent the hyphae of fungi occurring within the fructifications. © 2015 by The Mycological Society of America.

  10. Structure and stability of complex coacervate core micelles with lysozyme

    NARCIS (Netherlands)

    Lindhoud, Saskia; de Vries, Renko; Norde, Willem; Cohen Stuart, Martien A.

    Encapsulation of enzymes by polymers is a promising method to influence their activity and stability. Here, we explore the use of complex coacervate core micelles for encapsulation of enzymes. The core of the micelles consists of negatively charged blocks of the diblock copolymer PAA(42)PAAm(417)

  11. Structure and Stability of Complex Coacervate Core Micelles with Lysozyme

    NARCIS (Netherlands)

    Lindhoud, Saskia; de Vries, Renko; Norde, Willem; Cohen Stuart, Martinus Abraham

    2007-01-01

    Encapsulation of enzymes by polymers is a promising method to influence their activity and stability. Here, we explore the use of complex coacervate core micelles for encapsulation of enzymes. The core of the micelles consists of negatively charged blocks of the diblock copolymer PAA42PAAm417 and

  12. Structure and stability of complex coacervate core micelles with lysozyme

    NARCIS (Netherlands)

    Lindhoud, S.; Vries, de R.J.; Norde, W.; Cohen Stuart, M.A.

    2007-01-01

    Encapsulation of enzymes by polymers is a promising method to influence their activity and stability. Here, we explore the use of complex coacervate core micelles for encapsulation of enzymes. The core of the micelles consists of negatively charged blocks of the diblock copolymer PAA42PAAm417 and th

  13. Structure and Stability of Complex Coacervate Core Micelles with Lysozyme

    NARCIS (Netherlands)

    Lindhoud, Saskia; Vries, de Renko; Norde, Willem; Cohen Stuart, Martien A.

    2007-01-01

    Encapsulation of enzymes by polymers is a promising method to influence their activity and stability. Here, we explore the use of complex coacervate core micelles for encapsulation of enzymes. The core of the micelles consists of negatively charged blocks of the diblock copolymer PAA42PAAm417 and th

  14. Structure and stability of complex coacervate core micelles with lysozyme

    NARCIS (Netherlands)

    Lindhoud, Saskia; de Vries, Renko; Norde, Willem; Cohen Stuart, Martien A.

    2007-01-01

    Encapsulation of enzymes by polymers is a promising method to influence their activity and stability. Here, we explore the use of complex coacervate core micelles for encapsulation of enzymes. The core of the micelles consists of negatively charged blocks of the diblock copolymer PAA(42)PAAm(417) an

  15. Encapsulation into complex coacervate core micelles promotes EGFP dimerization

    NARCIS (Netherlands)

    Nolles, A.; Dongen, Van N.J.E.; Westphal, A.H.; Visser, A.J.W.G.; Kleijn, J.M.; Berkel, Van W.J.H.; Borst, J.W.

    2017-01-01

    Complex coacervate core micelles (C3Ms) are colloidal structures useful for encapsulation of biomacromolecules. We previously demonstrated that enhanced green fluorescent protein (EGFP) can be encapsulated into C3Ms using the diblock copolymer

  16. Revised

    DEFF Research Database (Denmark)

    Johannsen, Vivian Kvist; Nord-Larsen, Thomas; Riis-Nielsen, Torben;

    This report is a revised analysis of the Danish data on CO2 emissions from forest, afforestation and deforestation for the period 1990 - 2008 and a prognosis for the period until 2020. Revision have included measurements from 2009 in the estimations. The report is funded by the Ministry of Climate...

  17. Connecting core percolation and controllability of complex networks.

    Science.gov (United States)

    Jia, Tao; Pósfai, Márton

    2014-06-20

    Core percolation is a fundamental structural transition in complex networks related to a wide range of important problems. Recent advances have provided us an analytical framework of core percolation in uncorrelated random networks with arbitrary degree distributions. Here we apply the tools in analysis of network controllability. We confirm analytically that the emergence of the bifurcation in control coincides with the formation of the core and the structure of the core determines the control mode of the network. We also derive the analytical expression related to the controllability robustness by extending the deduction in core percolation. These findings help us better understand the interesting interplay between the structural and dynamical properties of complex networks.

  18. Out-of-Core Solutions of Complex Sparse Linear Equations

    Science.gov (United States)

    Yip, E. L.

    1982-01-01

    ETCLIB is library of subroutines for obtaining out-of-core solutions of complex sparse linear equations. Routines apply to dense and sparse matrices too large to be stored in core. Useful for solving any set of linear equations, but particularly useful in cases where coefficient matrix has no special properties that guarantee convergence with any of interative processes. The only assumption made is that coefficient matrix is not singular.

  19. The radiological consequences of degraded core accidents for the Sizewell PWR The impact of adopting revised frequencies of occurrence

    CERN Document Server

    Kelly, G N

    1983-01-01

    The radiological consequences of degraded core accidents postulated for the Sizewell PWR were assessed in an earlier study and the results published in NRPB-R137. Further analyses have since been made by the Central Electricity Generating Board (CEGB) of degraded core accidents which have led to a revision of their predicted frequencies of occurrence. The implications of these revised frequencies, in terms of the risk to the public from degraded core accidents, are evaluated in this report. Increases, by factors typically within the range of about 1.5 to 7, are predicted in the consequences, compared with those estimated in the earlier study. However, the predicted risk from degraded core accidents, despite these increases, remains exceedingly small.

  20. Structure and stability of complex coacervate core micelles with lysozyme.

    Science.gov (United States)

    Lindhoud, Saskia; Vries, Renko de; Norde, Willem; Stuart, Martien A Cohen

    2007-07-01

    Encapsulation of enzymes by polymers is a promising method to influence their activity and stability. Here, we explore the use of complex coacervate core micelles for encapsulation of enzymes. The core of the micelles consists of negatively charged blocks of the diblock copolymer PAA42PAAm417 and the positively charged homopolymer PDMAEMA150. For encapsulation, part of the positively charged homopolymer was replaced by the positively charged globular protein lysozyme. We have studied the formation, structure, and stability of the resulting micelles for three different mixing ratios of homopolymer and lysozyme: a system predominantly consisting of homopolymer, a system predominantly consisting of lysozyme, and a system where the molar ratio between the two positively charged molecules was almost one. We also studied complexes made of only lysozyme and PAA42PAAm417. Complex formation and the salt-induced disintegration of the complexes were studied using dynamic light-scattering titrations. Small-angle neutron scattering was used to investigate the structures of the cores. We found that micelles predominantly consisting of homopolymer are spherical but that complex coacervate core micelles predominantly consisting of lysozyme are nonspherical. The stability of the micelles containing a larger fraction of lysozyme is lower.

  1. Complex coacervate core micelles with a lysozyme-modified corona

    NARCIS (Netherlands)

    Danial, M.; Klok, H.A.; Norde, W.; Cohen Stuart, M.A.

    2007-01-01

    This paper describes the preparation, characterization, and enzymatic activity of complex coacervate core micelles (C3Ms) composed of poly(acrylic acid) (PAA) and poly(N-methyl-2-vinyl pyridinium iodide)-b-poly(ethylene oxide) (PQ2VP-PEO) to which the antibacterial enzyme lysozyme is end-attached.

  2. Complex coacervation core micelles. Colloidal stability and aggregation mechanism

    NARCIS (Netherlands)

    Burgh, van der S.; Keizer, de A.; Cohen Stuart, M.A.

    2004-01-01

    Complex coacervation core micelles were prepared with various polyelectrolytes and oppositely charged diblock copolymers. The diblock copolymers consist of a charged block and a water-soluble neutral block. Our experimental technique was dynamic light scattering in combination with titrations. At

  3. GluR2 ligand-binding core complexes

    DEFF Research Database (Denmark)

    Kasper, C; Lunn, M-L; Liljefors, T

    2002-01-01

    X-ray structures of the GluR2 ligand-binding core in complex with (S)-Des-Me-AMPA and in the presence and absence of zinc ions have been determined. (S)-Des-Me-AMPA, which is devoid of a substituent in the 5-position of the isoxazolol ring, only has limited interactions with the partly hydrophobic...

  4. Core promoter recognition complex changes accompany liver development

    Science.gov (United States)

    D’Alessio, Joseph A.; Ng, Raymond; Willenbring, Holger; Tjian, Robert

    2011-01-01

    Recent studies of several key developmental transitions have brought into question the long held view of the basal transcriptional apparatus as ubiquitous and invariant. In an effort to better understand the role of core promoter recognition and coactivator complex switching in cellular differentiation, we have examined changes in transcription factor IID (TFIID) and cofactor required for Sp1 activation/Mediator during mouse liver development. Here we show that the differentiation of fetal liver progenitors to adult hepatocytes involves a wholesale depletion of canonical cofactor required for Sp1 activation/Mediator and TFIID complexes at both the RNA and protein level, and that this alteration likely involves silencing of transcription factor promoters as well as protein degradation. It will be intriguing for future studies to determine if a novel and as yet unknown core promoter recognition complex takes the place of TFIID in adult hepatocytes and to uncover the mechanisms that down-regulate TFIID during this critical developmental transition. PMID:21368148

  5. The synthesis, design and applications of lanthanide cored complexes

    Science.gov (United States)

    Phelan, Gregory David

    Novel luminescent materials based on lanthanide cored complexes have been designed and synthesized. The complexes consist of a beta-diketone ligand chelated to a lanthanide metal such as europium or gadolinium. A series of beta-diketone ligands were designed and synthesized. The ligands consist of a polycyclic aromatic sensitizer, phenanthrene, and a second functional group. The second groups consisted of another unit of phenanthrene, a dendritic structure, or a fluorinated alkyl chain. The europium complexes have been incorporated into organic light emitting devices that have a major emission at 615 nm and a maximum brightness of 300 cd/m2. The gadolinium complexes were used to dope into the resulting organic light emitting devices to help improve the efficiency of the device. The use of the gadolinium complexes results in a 25 fold increase in efficiency.

  6. Core promoter recognition complex changes accompany liver development

    OpenAIRE

    D’Alessio, Joseph A.; Ng, Raymond; Willenbring, Holger; Tjian, Robert

    2011-01-01

    Recent studies of several key developmental transitions have brought into question the long held view of the basal transcriptional apparatus as ubiquitous and invariant. In an effort to better understand the role of core promoter recognition and coactivator complex switching in cellular differentiation, we have examined changes in transcription factor IID (TFIID) and cofactor required for Sp1 activation/Mediator during mouse liver development. Here we show that the differentiation of fetal li...

  7. Complexities of revision mastoid surgery in a migratory population cohort.

    LENUS (Irish Health Repository)

    Lennon, P

    2012-03-01

    We present a review of patients from Eastern Europe who have recently immigrated to Ireland with complicated otological disease. We carried out a retrospective chart review of these patients. These are a complicated cohort of 7 patients, 5 (71.4%) of whom had previous ear surgery, none had old notes and there was often a need for interpreters (3 or 42.8%) and challenging surgery. Follow up was also problematic with many of the patients. In summary this is a case series to highlight a relatively new group of patients to emphasize the need for pre-operative CT scans, facial nerve monitoring and the difficult nature of revision surgery with no old notes.

  8. Towards a Molecular Understanding of the Fanconi Anemia Core Complex

    Directory of Open Access Journals (Sweden)

    Charlotte Hodson

    2012-01-01

    Full Text Available Fanconi Anemia (FA is a genetic disorder characterized by the inability of patient cells to repair DNA damage caused by interstrand crosslinking agents. There are currently 14 verified FA genes, where mutation of any single gene prevents repair of DNA interstrand crosslinks (ICLs. The accumulation of ICL damage results in genome instability and patients having a high predisposition to cancers. The key event of the FA pathway is dependent on an eight-protein core complex (CC, required for the monoubiquitination of each member of the FANCD2-FANCI complex. Interestingly, the majority of patient mutations reside in the CC. The molecular mechanisms underlying the requirement for such a large complex to carry out a monoubiquitination event remain a mystery. This paper documents the extensive efforts of researchers so far to understand the molecular roles of the CC proteins with regard to its main function in the FA pathway, the monoubiquitination of FANCD2 and FANCI.

  9. THE ORIGIN OF COMPLEX ORGANIC MOLECULES IN PRESTELLAR CORES

    Energy Technology Data Exchange (ETDEWEB)

    Vastel, C. [Université de Toulouse, UPS-OMP, IRAP, F-31400 Toulouse (France); Ceccarelli, C.; Lefloch, B. [Univ. Grenoble Alpes, IPAG, F-38000 Grenoble (France); Bachiller, R., E-mail: cvastel@irap.omp.eu [Observatorio Astronómico Nacional (OAN, IGN). Calle Alfonso XII 3, E-28014 Madrid (Spain)

    2014-11-01

    Complex organic molecules (COMs) have been detected in a variety of environments including cold prestellar cores. Given the low temperatures of these objects, these detections challenge existing models. We report here new observations toward the prestellar core L1544. They are based on an unbiased spectral survey of the 3 mm band at the IRAM 30 m telescope as part of the Large Program ASAI. The observations allow us to provide a full census of the oxygen-bearing COMs in this source. We detected tricarbon monoxide, methanol, acetaldehyde, formic acid, ketene, and propyne with abundances varying from 5 × 10{sup –11} to 6 × 10{sup –9}. The non-LTE analysis of the methanol lines shows that they are likely emitted at the border of the core at a radius of ∼8000 AU, where T ∼ 10 K and n {sub H{sub 2}} ∼2 × 10{sup 4} cm{sup –3}. Previous works have shown that water vapor is enhanced in the same region because of the photodesorption of water ices. We propose that a non-thermal desorption mechanism is also responsible for the observed emission of methanol and COMs from the same layer. The desorbed oxygen and a small amount of desorbed methanol and ethene are enough to reproduce the abundances of tricarbon monoxide, methanol, acetaldehyde, and ketene measured in L1544. These new findings open the possibility that COMs in prestellar cores originate in a similar outer layer rather than in the dense inner cores, as previously assumed, and that their formation is driven by the non-thermally desorbed species.

  10. Structural characterization of core-bradavidin in complex with biotin

    Science.gov (United States)

    Agrawal, Nitin; Määttä, Juha A. E.; Kulomaa, Markku S.; Hytönen, Vesa P.; Johnson, Mark S.; Airenne, Tomi T.

    2017-01-01

    Bradavidin is a tetrameric biotin-binding protein similar to chicken avidin and bacterial streptavidin, and was originally cloned from the nitrogen-fixing bacteria Bradyrhizobium diazoefficiens. We have previously reported the crystal structure of the full-length, wild-type (wt) bradavidin with 138 amino acids, where the C-terminal residues Gly129-Lys138 (“Brad-tag”) act as an intrinsic ligand (i.e. Gly129-Lys138 bind into the biotin-binding site of an adjacent subunit within the same tetramer) and has potential as an affinity tag for biotechnological purposes. Here, the X-ray structure of core-bradavidin lacking the C-terminal residues Gly114-Lys138, and hence missing the Brad-tag, was crystallized in complex with biotin at 1.60 Å resolution [PDB:4BBO]. We also report a homology model of rhodavidin, an avidin-like protein from Rhodopseudomonas palustris, and of an avidin-like protein from Bradyrhizobium sp. Ai1a-2, both of which have the Brad-tag sequence at their C-terminus. Moreover, core-bradavidin V1, an engineered variant of the original core-bradavidin, was also expressed at high levels in E. coli, as well as a double mutant (Cys39Ala and Cys69Ala) of core-bradavidin (CC mutant). Our data help us to further engineer the core-bradavidin–Brad-tag pair for biotechnological assays and chemical biology applications, and provide deeper insight into the biotin-binding mode of bradavidin. PMID:28426764

  11. Revised RPS Method for Complex Solution of Multimode Slab Waveguides with Metal layers

    Institute of Scientific and Technical Information of China (English)

    LI Jian-jun; SHEN Guang-di

    2004-01-01

    The RPS method,which integrates the real numerical solution, the perturbation solution and the shooting solution reasonably,can obtain the complex solution of a slab waveguide with small gain/loss regions without searching the root in the entire complex plan. A revision to the RPS method is presented so that it can deal with the waveguide including large gain/loss regions. The application indicated that the simulation results are precise.

  12. The origin of complex organic molecules in prestellar cores

    CERN Document Server

    Vastel, Charlotte; Lefloch, Bertrand; Bachiller, Raphael

    2014-01-01

    Complex organic molecules (COMs) have been detected in a variety of environments, including cold prestellar cores. Given the low temperature of these objects, these last detections challenge existing models. We report here new observations towards the prestellar core L1544. They are based on an unbiased spectral survey of the 3mm band at the IRAM-30m telescope, as part of the Large Program ASAI. The observations allow us to provide the full census of the oxygen bearing COMs in this source. We detected tricarbon monoxide, methanol, acetaldehyde, formic acid, ketene, and propyne with abundances varying from 5e-11 to 6e-9. The non-LTE analysis of the methanol lines shows that they are likely emitted at the border of the core, at a radius of ~8000 AU where T~10 K and nH2~2e4 cm-3. Previous works have shown that water vapour is enhanced in the same region because of the photodesorption of water ices. We propose that a non-thermal desorption mechanism is also responsible for the observed emission of methanol and CO...

  13. Structural and Biochemical Insights into MLL1 Core Complex Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Avdic, Vanja; Zhang, Pamela; Lanouette, Sylvain; Groulx, Adam; Tremblay, Véronique; Brunzelle, Joseph; Couture, Jean-François (Ottawa); (NWU)

    2012-05-02

    Histone H3 Lys-4 methylation is predominantly catalyzed by a family of methyltransferases whose enzymatic activity depends on their interaction with a three-subunit complex composed of WDR5, RbBP5, and Ash2L. Here, we report that a segment of 50 residues of RbBP5 bridges the Ash2L C-terminal domain to WDR5. The crystal structure of WDR5 in ternary complex with RbBP5 and MLL1 reveals that both proteins binds peptide-binding clefts located on opposite sides of WDR5s {beta}-propeller domain. RbBP5 engages in several hydrogen bonds and van der Waals contacts within a V-shaped cleft formed by the junction of two blades on WDR5. Mutational analyses of both the WDR5 V-shaped cleft and RbBP5 residues reveal that the interactions between RbBP5 and WDR5 are important for the stimulation of MLL1 methyltransferase activity. Overall, this study provides the structural basis underlying the formation of the WDR5-RbBP5 subcomplex and further highlight the crucial role of WDR5 in scaffolding the MLL1 core complex.

  14. Relationships Between Complex Core Level Spectra and Materials Properties

    Energy Technology Data Exchange (ETDEWEB)

    Nelin, Constance J.; Bagus, Paul S.; Ilton, Eugene S.; Chambers, Scott A.; Kuhlenbeck, Helmut; Freund, Hans-Joachim

    2010-12-01

    The XPS of many oxides are quite complex and there may be several peaks of significant intensity for each subshell. These peaks arise from many-electron effects, which normally are treated with configuration interaction (CI) wavefunctions where static correlation effects are taken into account. It is common to use semiempirical methods to determine the matrix elements of the CI Hamiltonian and there are few rigorous CI calculations where parameters are not adjusted to fit experiment. In contrast, we present, in the present work, theoretical XPS spectra obtained with rigorous CI wavefunctions for CeO2 where the XPS are especially complex; several different core levels are studied. This study uses an embedded CeO8 cluster model to represent bulk CeO2 and the relativistic CI wavefunctions are determined using four-component spinors from Dirac-Fock calculations. In particular, we examine the importance of interatomic many-body effects where there is a transfer of electrons from occupied oxygen 2p orbitals into empty cation orbitals as it is common to ascribe the complex XPS to this effect. We also contrast the importance of many-body charge-transfer effects for the isoelectronic cations of Ce4+ and La3+. The long-range goal of this work is to relate the XPS features to the nature of the chemical bonding in CeO2 and we describe our progress toward this goal.

  15. Stability of complex coacervate core micelles containing metal coordination polymer.

    Science.gov (United States)

    Yan, Yun; de Keizer, Arie; Cohen Stuart, Martien A; Drechsler, Markus; Besseling, Nicolaas A M

    2008-09-01

    We report on the stability of complex coacervate core micelles, i.e., C3Ms (or PIC, BIC micelles), containing metal coordination polymers. In aqueous solutions these micelles are formed between charged-neutral diblock copolymers and oppositely charged coordination polymers formed from metal ions and bisligand molecules. The influence of added salt, polymer concentration, and charge composition was investigated by using light scattering and cryo-TEM techniques. The scattering intensity decreases strongly with increasing salt concentration until a critical salt concentration beyond which no micelles exist. The critical micelle concentration increases almost exponentially with the salt concentration. From the scattering results it follows that the aggregation number decreases with the square root of the salt concentration, but the hydrodynamic radius remains constant or increases slightly. It was concluded that the density of the core decreases with increasing ionic strength. This is in agreement with theoretical predictions and is also confirmed by cryo-TEM measurements. A complete composition diagram was constructed based on the composition boundaries obtained from light scattering titrations.

  16. Passive Seismic Imaging of the Ruby Mountains Core Complex, Nevada

    Science.gov (United States)

    Litherland, M.; Klemperer, S. L.

    2015-12-01

    We investigate the deep crustal structure of the Ruby Mountains Core Complex (RMCC) using data collected from the Ruby Mountains Seismic Experiment. This project, part of the Earthscope Flexible Array program, deployed 50 passive broadband stations across the RMCC from 2010 to 2012. Previous investigations of the area have included extensive surface mapping and active seismic profiles across the surrounding basins, but better imaging beneath the mountain range is needed to understand the tectonic processes that formed the RMCC. The RMCC exhibits typical core-complex structure of deep crustal rocks exhumed to the surface beneath a gently dipping detachment, with a thick mylonitic shear zone directly underlying the detachment. In the RMCC, the westward dip of the detachment, the ~1km-thick mylonite zone formed in the Paleogene, and a south-to-north increase in metamorphic grade provide targets for imaging. We used common conversion point stacking of receiver functions to produce 3 profiles of structural discontinuities beneath the RMCC: one along the axis of the RMCC, and two crossing lines, one in the northern RMCC, and one in the southern part of the range. Due to the deep sedimentary basins surrounding the RMCC, various de-multiple processes were required to reduce the effects of basin reverberations. To better constrain the velocity structure of the area, we used ambient-noise tomography, and finally, we produced a joint inversion of our receiver functions and ambient-noise data. We observe a mostly flat Moho at about 30 km depth beneath the RMCC that dips slightly to the south, with faint mid-crustal converters that also dip south at ~30°. In the southern RMCC, the Moho dips ~20° westward, but this is not observed in the northern RMCC. This suggests that much of the exhumation involved in the RMCC formation likely involved ductile flow that left a mostly flat Moho, but more recent processes also may have left observable changes in lower-crustal structure.

  17. Complex coacervate core micelles with a lysozyme-modified corona.

    Science.gov (United States)

    Danial, Maarten; Klok, Harm-Anton; Norde, Willem; Stuart, Martien A Cohen

    2007-07-17

    This paper describes the preparation, characterization, and enzymatic activity of complex coacervate core micelles (C3Ms) composed of poly(acrylic acid) (PAA) and poly(N-methyl-2-vinyl pyridinium iodide)-b-poly(ethylene oxide) (PQ2VP-PEO) to which the antibacterial enzyme lysozyme is end-attached. C3Ms were prepared by polyelectrolyte complex formation between PAA and mixtures containing different ratios of aldehyde and hydroxyl end-functionalized PQ2VP-PEO. This resulted in the formation of C3Ms containing 0-40% (w/w) of the aldehyde end-functionalized PQ2VP-PEO block copolymer (PQ2VP-PEO-CHO). Chemical conjugation of lysozyme was achieved via reductive amination of the aldehyde groups, which are exposed at the surface of the C3M, with the amine groups present in the side chains of the lysine residues of the protein. Dynamic and static light scattering indicated that the conjugation of lysozyme to C3Ms prepared using 10 and 20% (w/w) PQ2VP-PEO-CHO resulted in the formation of unimicellar particles. Multimicellar aggregates, in contrast, were obtained when lysozyme was conjugated to C3Ms prepared using 30 or 40% (w/w) PQ2VP-PEO-CHO. The enzymatic activity of the unimicellar lysozyme-C3M conjugates toward the hydrolysis of the bacterial substrate Micrococcus lysodeikticus was comparable to that of free lysozyme. For the multimicellar particles, in contrast, significantly reduced enzymatic rates of hydrolysis, altered circular dichroism, and red-shifted tryptophan fluorescence spectra were measured. These results are attributed to the occlusion of lysozyme in the interior of the multimicellar conjugates.

  18. Encapsulation of GFP in Complex Coacervate Core Micelles.

    Science.gov (United States)

    Nolles, Antsje; Westphal, Adrie H; de Hoop, Jacob A; Fokkink, Remco G; Kleijn, J Mieke; van Berkel, Willem J H; Borst, Jan Willem

    2015-05-11

    Protein encapsulation with polymers has a high potential for drug delivery, enzyme protection and stabilization. Formation of such structures can be achieved by the use of polyelectrolytes to generate so-called complex coacervate core micelles (C3Ms). Here, encapsulation of enhanced green fluorescent protein (EGFP) was investigated using a cationic-neutral diblock copolymer of two different sizes: poly(2-methyl-vinyl-pyridinium)41-b-poly(ethylene-oxide)205 and poly(2-methyl-vinyl-pyridinium)128-b-poly(ethylene-oxide)477. Dynamic light scattering and fluorescence correlation spectroscopy (FCS) revealed a preferred micellar composition (PMC) with a positive charge composition of 0.65 for both diblock copolymers and micellar hydrodynamic radii of approximately 34 nm. FCS data show that at the PMC, C3Ms are formed above 100 nM EGFP, independent of polymer length. Mixtures of EGFP and nonfluorescent GFP were used to quantify the amount of GFP molecules per C3M, resulting in approximately 450 GFPs encapsulated per micelle. This study shows that FCS can be successfully applied for the characterization of protein-containing C3Ms.

  19. Complex coacervate core micelles from iron-based coordination polymers.

    Science.gov (United States)

    Wang, Junyou; de Keizer, Arie; Fokkink, Remco; Yan, Yun; Cohen Stuart, Martien A; van der Gucht, Jasper

    2010-07-01

    Complex coacervate core micelles (C3Ms) from cationic poly(N-methyl-2-vinyl-pyridinium iodide)-b-poly(ethylene oxide) (P2MVP(41)-b-PEO(205)) and anionic iron coordination polymers are investigated in the present work. Micelle formation is studied by light scattering for both Fe(II)- and Fe(III)-containing C3Ms. At the stoichiometric charge ratio, both Fe(II)-C3Ms and Fe(III)-C3Ms are stable for at least 1 week at room temperature. Excess of iron coordination polymers has almost no effect on the formed Fe(II)-C3Ms and Fe(III)-C3Ms, whereas excess of P2MVP(41)-b-PEO(205) copolymers in the solution can dissociate the formed micelles. Upon increasing salt concentration, the scattering intensity decreases. This decrease is due to both a decrease in the number of micelles (or an increase in CMC) and a decrease in aggregation number. The salt dependence of the CMC and the aggregation number is explained using a scaling argument for C3M formation. Compared with Fe(II)-C3Ms, Fe(III)-C3Ms have a lower CMC and a higher stability against dissociation by added salt.

  20. Polyoxometalate complexes of anatase-titanium dioxide cores in water.

    Science.gov (United States)

    Raula, Manoj; Gan Or, Gal; Saganovich, Marina; Zeiri, Offer; Wang, Yifeng; Chierotti, Michele R; Gobetto, Roberto; Weinstock, Ira A

    2015-10-12

    Polyoxometalate (POM) cluster anions are shown to serve as covalently coordinated ligands for anatase-TiO2 nanocrystals, giving isolable assemblies uniquely positioned between molecular macroanions and traditional colloidal nanoparticles. Na(+) salts of the water-soluble polyanionic structures are obtained by reacting amorphous TiO2 with the 1 nm lacunary ion, Na7 [α-XW11 O39 ] (X=P(5+) ), at 170 °C, after which an average of 55 α-PW11 O39 (7-) clusters are found as pentadentate ligands for Ti(IV) ions covalently linked to 6 nm single-crystal anatase cores. The attached POMs are reversible electron acceptors, the reduction potentials of which shift in a predictable fashion by changing the central heteroatom, X, directly influencing a model catalytic reaction. Just as POM cluster anions control the reactivities of metal centers in molecular complexes, directly coordinated POM ligands with tunable redox potentials now provide new options for rationally controlling the reactions of semiconductor nanocrystals.

  1. FEM simulation of formation of metamorphic core complex with ANSYS software

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This study utilizes ANSYS to establish FEM's model of metamorphic core complex,and used thermal-structure analysis to simulate metamorphic core complex's temperature field and stress field.The metamorphic core complex formation mechanism is discussed.The simulation results show that the temperature field change appearing as the earth surface's temperature is the lowest,and the temperature of metamorphic core complex's nucleus is the highest.The temperature field is higher along with depth increase,and the stress field change appearing as the biggest stress occurs in the nucleus.The next stress field occurs at the top of the cover.

  2. The 2012 American College of Nurse-Midwives core competencies for basic midwifery practice: history and revision.

    Science.gov (United States)

    Phillippi, Julia C; Avery, Melissa D

    2014-01-01

    The American College of Nurse-Midwives (ACNM) Core Competencies for Basic Midwifery Practice, approved in 2012, (hereafter referred to as Core Competencies) outline the knowledge, skills, and abilities that can be expected of new certified nurse-midwives (CNMs) and certified midwives (CMs). The Core Competencies are standards for midwifery education, and the document is an important guide for midwifery practice and policy. As a part of the 2012 revision, the Basic Competency Section of the ACNM Division of Education reviewed a variety of national and international documents to ensure that the basic education of CNMs/CMs is consistent with the practice of midwives in the United States and internationally. Few substantive changes were made to the document, but several areas were adjusted and clarified. New graduates continue to be prepared by midwifery education programs to provide safe, evidence-based midwifery care to women across the lifespan, well newborns up to 28 days, and sexual partners of women diagnosed with sexually transmitted infections. © 2014 by the American College of Nurse-Midwives.

  3. Activities for Challenging Gifted Learners by Increasing Complexity in the Common Core

    Science.gov (United States)

    McKeone, Alyssa; Caruso, Lenora; Bettle, Kailyn; Chase, Ashley; Bryson, Bridget; Schneider, Jean S.; Rule, Audrey C.

    2015-01-01

    Gifted learners need opportunities for critical and creative thinking to stretch their minds and imaginations. Strategies for increasing complexity in the four core areas of language arts, mathematics, science, and social studies were addressed using the Common Core and Iowa Core Standards through several methods. Descriptive adjective object…

  4. Thermal stability of oxygen evolution in photosystem Ⅱ core complex in the presence of digalactosyl diacylglycerol

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ (PSⅡ) core complex. It was found that the temperature of semi-inactivation of oxygen evolution in the complex increased from 40.0 to about 43.0℃ in the presence of DGDG with 5-min heat treatment in the dark. Furthermore, when PSⅡ core complex was incubated for 5 min at 45.0℃, the oxygen evolution in the complex was completely lost, whilst the DGDG-complexed PSⅡ core complex still retained a 16% of activity (100% for 25.0℃). In addition, a 1-h incubation at 38.0℃ inactivated absolutely the oxygen evolution for the PSⅡ core complex. By contrast, there remained about 20% of activity (zero time for 100%) for the complex in the presence of DGDG under the same condition. These results indicate a new role of DGDG in the protection of PSⅡ core complex against the deleterious effects of temperature. It was most likely that DGDG-mediated stability toward thermal denaturation of oxygen evolution in PSⅡ core complex is due to the protective effect of DGDG on the release of the 33 kD protein from PSⅡ core complex.

  5. VENUS-2 MOX Core Benchmark: Results of ORNL Calculations Using HELIOS-1.4 - Revised Report

    Energy Technology Data Exchange (ETDEWEB)

    Ellis, RJ

    2001-06-01

    The Task Force on Reactor-Based Plutonium Disposition (TFRPD) was formed by the Organization for Economic Cooperation and Development/Nuclear Energy Agency (OECD/NEA) to study reactor physics, fuel performance, and fuel cycle issues related to the disposition of weapons-grade (WG) plutonium as mixed-oxide (MOX) reactor fuel. To advance the goals of the TFRPD, 10 countries and 12 institutions participated in a major TFRPD activity: a blind benchmark study to compare code calculations to experimental data for the VENUS-2 MOX core at SCK-CEN in Mol, Belgium. At Oak Ridge National Laboratory, the HELIOS-1.4 code system was used to perform the comprehensive study of pin-cell and MOX core calculations for the VENUS-2 MOX core benchmark study.

  6. Career and Personal Development for Plant Science Core Curriculum. Instructor's Guide. Vol. 15. No. 1. Revised.

    Science.gov (United States)

    Stewart, Bob R.; And Others

    Intended as part of the second year of instruction for students of vocational agriculture, this unit for a plant science core curriculum consists of five lessons designed to help students in their careers and personal development. Topics are: reconsidering career plans; finding information on available jobs; getting a job; developing as a…

  7. The Liaonan Metamorphic Core Complex: Constitution, Structure and Evolution

    Institute of Scientific and Technical Information of China (English)

    LIU Junlai; GUAN Huimei; JI Mo; CAO Shuyun; HU Ling

    2006-01-01

    The Liaonan metamorphic core complex (mcc) has a three-layer structure and is constituted by five parts, i.e. a detachment fault zone, an allochthonous upper plate and an supradetachment basin above the fault zone, and highly metamorphosed rocks and intrusive rocks in the lower plate. The allochthonous upper plate is mainly of Neoproterozoic and Paleozoic rocks weakly deformed and metamorphosed in pre-Indosinan stage. Above these rocks is a small-scale supradetachment basin of Cretaceous sedimentary and volcanic rocks. The lower plate is dominated by Archean TTG gneisses with minor amount of supracrustal rocks. The Archean rocks are intruded by late Mesozoic synkinematic monzogranitic and granitic plutons. Different types of fault rocks, providing clues to the evolution of the detachment fault zone, are well-preserved in the fault zone, e.g. mylonitic gneiss,mylonites, brecciated mylonites, microbreccias and pseudotachylites. Lineations in lower plate granitic intrusions have consistent orientation that indicate uniform top-to-NW shearing along the main detachment fault zone. This also provides evidence for the synkinematic characteristics of the granitic plutons in the lower plate. Structural analysis of the different parts in the mcc and isotopic dating of plutonic rocks from the lower plate and mylonitic rocks from detachment fault zone suggest that exhumation of the mcc started with regional crustal extension due to crustal block rotation and tangential shearing. The extension triggered magma formation, upwelling and emplacement. This event ended with appearance of pseudotachylite and fault gauges formed at the uppermost crustal level.U-Pb dating of single zircon grains from granitic rocks in the lower plate gives an age of 130±5 Ma, and biotite grains from the main detachment fault zone have 40Ar-39Ar ages of 108-119 Ma. Several aspects may provide constraints for the exhumation of the Liaonan mcc. These include regional extensional setting, cover

  8. Revised estimates of Greenland ice sheet thinning histories based on ice-core records

    DEFF Research Database (Denmark)

    Lecavalier, B.S.; Milne, G.A.; Fisher, D.A.;

    2013-01-01

    -based reconstructions and, to some extent, the estimated elevation histories. A key component of the ice core analysis involved removing the influence of vertical surface motion on the dO signal measured from the Agassiz and Renland ice caps. We re-visit the original analysis with the intent to determine if the use...... height changes on the dO signal from the two ice cores. This procedure is complicated by the fact that dO contained in Agassiz ice is influenced by land height changes distant from the ice cap and so selecting a single location at which to compute the land height signal is not possible. Uncertainty...... in this selection is further complicated by the possible influence of Innuitian ice during the early Holocene (12-8 ka BP). Our results indicate that a more accurate treatment of the uplift correction leads to elevation histories that are, in general, shifted down relative to the original curves at GRIP, NGRIP, DYE...

  9. 3D Printing Aids Acetabular Reconstruction in Complex Revision Hip Arthroplasty

    Directory of Open Access Journals (Sweden)

    Andrew J. Hughes

    2017-01-01

    Full Text Available Revision hip arthroplasty requires comprehensive appreciation of abnormal bony anatomy. Advances in radiology and manufacturing technology have made three-dimensional (3D representation of osseous anatomy obtainable, which provide visual and tactile feedback. Such life-size 3D models were manufactured from computed tomography scans of three hip joints in two patients. The first patient had undergone multiple previous hip arthroplasties for bilateral hip infections, resulting in right-sided pelvic discontinuity and a severe left-sided posterosuperior acetabular deficiency. The second patient had a first-stage revision for infection and recurrent dislocations. Specific metal reduction protocols were used to reduce artefact. The images were imported into Materialise MIMICS 14.12®. The models were manufactured using selective laser sintering. Accurate templating was performed preoperatively. Acetabular cup, augment, buttress, and cage sizes were trialled using the models, before being adjusted, and resterilised, enhancing the preoperative decision-making process. Screw trajectory simulation was carried out, reducing the risk of neurovascular injury. With 3D printing technology, complex pelvic deformities were better evaluated and treated with improved precision. Life-size models allowed accurate surgical simulation, thus improving anatomical appreciation and preoperative planning. The accuracy and cost-effectiveness of the technique should prove invaluable as a tool to aid clinical practice.

  10. 3D Printing Aids Acetabular Reconstruction in Complex Revision Hip Arthroplasty

    Science.gov (United States)

    DeBuitleir, Cathal; Soden, Philip; O'Donnchadha, Brian; Tansey, Anthony; Abdulkarim, Ali; McMahon, Colm; Hurson, Conor J.

    2017-01-01

    Revision hip arthroplasty requires comprehensive appreciation of abnormal bony anatomy. Advances in radiology and manufacturing technology have made three-dimensional (3D) representation of osseous anatomy obtainable, which provide visual and tactile feedback. Such life-size 3D models were manufactured from computed tomography scans of three hip joints in two patients. The first patient had undergone multiple previous hip arthroplasties for bilateral hip infections, resulting in right-sided pelvic discontinuity and a severe left-sided posterosuperior acetabular deficiency. The second patient had a first-stage revision for infection and recurrent dislocations. Specific metal reduction protocols were used to reduce artefact. The images were imported into Materialise MIMICS 14.12®. The models were manufactured using selective laser sintering. Accurate templating was performed preoperatively. Acetabular cup, augment, buttress, and cage sizes were trialled using the models, before being adjusted, and resterilised, enhancing the preoperative decision-making process. Screw trajectory simulation was carried out, reducing the risk of neurovascular injury. With 3D printing technology, complex pelvic deformities were better evaluated and treated with improved precision. Life-size models allowed accurate surgical simulation, thus improving anatomical appreciation and preoperative planning. The accuracy and cost-effectiveness of the technique should prove invaluable as a tool to aid clinical practice. PMID:28168060

  11. Has First-Grade Core Reading Program Text Complexity Changed across Six Decades?

    Science.gov (United States)

    Fitzgerald, Jill; Elmore, Jeff; Relyea, Jackie Eunjung; Hiebert, Elfrieda H.; Stenner, A. Jackson

    2016-01-01

    The purpose of the study was to address possible text complexity shifts across the past six decades for a continually best-selling first-grade core reading program. The anthologies of one publisher's seven first-grade core reading programs were examined using computer-based analytics, dating from 1962 to 2013. Variables were Overall Text…

  12. Revised results for geomechanical testing of MRIG-9 core for the potential SPR siting at the Richton Salt Dome.

    Energy Technology Data Exchange (ETDEWEB)

    Broome, Scott Thomas; Bauer, Stephen J.

    2010-02-01

    This report is a revision of SAND2009-0852. SAND2009-0852 was revised because it was discovered that a gage used in the original testing was mis-calibrated. Following the recalibration, all affected raw data were recalculated and re-presented. Most revised data is similar to, but slightly different than, the original data. Following the data re-analysis, none of the inferences or conclusions about the data or site relative to the SAND2009-0852 data have been changed. A laboratory testing program was developed to examine the mechanical behavior of salt from the Richton salt dome. The resulting information is intended for use in design and evaluation of a proposed Strategic Petroleum Reserve storage facility in that dome. Core obtained from the drill hole MRIG-9 was obtained from the Texas Bureau of Economic Geology. Mechanical properties testing included: (1) acoustic velocity wave measurements; (2) indirect tensile strength tests; (3) unconfined compressive strength tests; (4) ambient temperature quasi-static triaxial compression tests to evaluate dilational stress states at confining pressures of 725, 1450, 2175, and 2900 psi; and (5) confined triaxial creep experiments to evaluate the time-dependent behavior of the salt at axial stress differences of 4000 psi, 3500 psi, 3000 psi, 2175 psi and 2000 psi at 55 C and 4000 psi at 35 C, all at a constant confining pressure of 4000 psi. All comments, inferences, discussions of the Richton characterization and analysis are caveated by the small number of tests. Additional core and testing from a deeper well located at the proposed site is planned. The Richton rock salt is generally inhomogeneous as expressed by the density and velocity measurements with depth. In fact, we treated the salt as two populations, one clean and relatively pure (> 98% halite), the other salt with abundant (at times) anhydrite. The density has been related to the insoluble content. The limited mechanical testing completed has allowed us to

  13. Core science and technology development plan for indirect-drive ICF ignition. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Powell, H.T.; Kilkenny, J.D. [eds.

    1995-12-01

    To define the development work needed to support inertial confinement fusion (ICF) program goals, the authors have assembled this Core Science and Technology (CS and T) Plan that encompasses nearly all science research and technology development in the ICF program. The objective of the CS and T Plan described here is to identify the development work needed to ensure the success of advanced ICF facilities, in particular the National Ignition Facility (NIF). This plan is intended as a framework to facilitate planning and coordination of future ICF programmatic activities. The CS and T Plan covers all elements of the ICF program including laser technology, optic manufacturing, target chamber, target diagnostics, target design and theory, target components and fabrication, and target physics experiments. The CS and T Plan has been divided into these seven different technology development areas, and they are used as level-1 categories in a work breakdown structure (WBS) to facilitate the organization of all activities in this plan. The scope of the CS and T Plan includes all research and development required to support the NIF leading up to the activation and initial operation as an indirect-drive facility. In each of the CS and T main development areas, the authors describe the technology and issues that need to be addressed to achieve NIF performance goals. To resolve all issues and achieve objectives, an extensive assortment of tasks must be performed in a coordinated and timely manner. The authors describe these activities and present planning schedules that detail the flow of work to be performed over a 10-year period corresponding to estimated time needed to demonstrate fusion ignition with the NIF. Besides the benefits to the ICF program, the authors also discuss how the commercial sector and the nuclear weapons science may profit from the proposed research and development program.

  14. Revision of the Archiloa genus complex with description of seven new Archilina species (Platyhelminthes, Proseriata) from the Mediterranean

    NARCIS (Netherlands)

    Martens, Paul M.; Curini-Galletti, Marco C.

    1994-01-01

    Based on morphological and karyological investigations, the genus complex Archiloa sensu Karling (1966) is revised. The group contains seven genera, two of which are here described as new: Archiloa de Beauchamp, 1910, Archilopsis Meixner, 1938, Mesoda Marcus, 1949, Monocelopsis Ax, 1951, Archilina A

  15. Revision of the Archiloa genus complex with description of seven new Archilina species (Platyhelminthes, Proseriata) from the Mediterranean

    NARCIS (Netherlands)

    Martens, Paul M.; Curini-Galletti, Marco C.

    1994-01-01

    Based on morphological and karyological investigations, the genus complex Archiloa sensu Karling (1966) is revised. The group contains seven genera, two of which are here described as new: Archiloa de Beauchamp, 1910, Archilopsis Meixner, 1938, Mesoda Marcus, 1949, Monocelopsis Ax, 1951, Archilina

  16. Spreading Effect in Industrial Complex Network Based on Revised Structural Holes Theory.

    Science.gov (United States)

    Xing, Lizhi; Ye, Qing; Guan, Jun

    2016-01-01

    This paper analyzed the spreading effect of industrial sectors with complex network model under perspective of econophysics. Input-output analysis, as an important research tool, focuses more on static analysis. However, the fundamental aim of industry analysis is to figure out how interaction between different industries makes impacts on economic development, which turns out to be a dynamic process. Thus, industrial complex network based on input-output tables from WIOD is proposed to be a bridge connecting accurate static quantitative analysis and comparable dynamic one. With application of revised structural holes theory, flow betweenness and random walk centrality were respectively chosen to evaluate industrial sectors' long-term and short-term spreading effect process in this paper. It shows that industries with higher flow betweenness or random walk centrality would bring about more intensive industrial spreading effect to the industrial chains they stands in, because value stream transmission of industrial sectors depends on how many products or services it can get from the other ones, and they are regarded as brokers with bigger information superiority and more intermediate interests.

  17. Spreading Effect in Industrial Complex Network Based on Revised Structural Holes Theory.

    Directory of Open Access Journals (Sweden)

    Lizhi Xing

    Full Text Available This paper analyzed the spreading effect of industrial sectors with complex network model under perspective of econophysics. Input-output analysis, as an important research tool, focuses more on static analysis. However, the fundamental aim of industry analysis is to figure out how interaction between different industries makes impacts on economic development, which turns out to be a dynamic process. Thus, industrial complex network based on input-output tables from WIOD is proposed to be a bridge connecting accurate static quantitative analysis and comparable dynamic one. With application of revised structural holes theory, flow betweenness and random walk centrality were respectively chosen to evaluate industrial sectors' long-term and short-term spreading effect process in this paper. It shows that industries with higher flow betweenness or random walk centrality would bring about more intensive industrial spreading effect to the industrial chains they stands in, because value stream transmission of industrial sectors depends on how many products or services it can get from the other ones, and they are regarded as brokers with bigger information superiority and more intermediate interests.

  18. Trauma, innocence and the core complex of dissociation.

    Science.gov (United States)

    Kalsched, Donald E

    2017-09-01

    Trauma survivors often lament that they have lost their innocence or lost their souls and that something vulnerable and whole about themselves has been 'broken' or annihilated. Yet when the psychotherapeutic relationship begins, and symbolic material from dreams and the transference emerges, discernible patterns become apparent, indicating that a core of innocence and vitality has not been totally lost or annihilated. On the contrary, it has been 'saved' by dissociation and its system of inner objects and their protective and/or persecutory narrative 'scripts' or 'schemas'. The dissociative system splits off a wounded, orphaned 'child' in the psyche and clinging to this 'child' is a penumbra of innocence that apparently must be preserved at all costs. Unfortunately the costs of preservation are high because such encapsulated innocence becomes malignant, and the inner world turns perverse and destructive. Only when the wounded, orphaned, and innocent part of the personality is allowed to suffer experience again - this time with the promise of a new outcome - can true healing of trauma occur. How to facilitate this authentic suffering in the face of powerful resistances thrown up by the 'system', will be the focus of this paper. © 2017, The Society of Analytical Psychology.

  19. Genesis of the Hongzhen metamorphic core complex and its tectonic implications

    Institute of Scientific and Technical Information of China (English)

    ZHU Guang; XIE ChengLong; XIANG BiWei; HU ZhaoQi; WANG YongSheng; LI Xing

    2007-01-01

    The Hongzhen metamorphic core complex is situated in the Yangtze plate to the east of the Dabie orogenic belt. Its ductile detachment zone in the foot wall overprints on the metamorphic complex of the Proterozoic Dongling Group. The present profile of the ductile shear zone with consistent SW-dipping mineral elongation lineation shows antiform and reversed S-shape from northeast to southwest respectively. Exposure structures, microstructures and quartz C-axis fabric all indicate top-to-SW movement for the ductile shear zone. Recrystallisation types of quartz and feldspar in the mylonites demonstrate that the shear zone was developed under the amphibolite facies condition and at mid-crust levels. The metamorphic core complex formed in the Early Cretaceous with a muscovite plateau age of 124.8±1.2 Ma. Regional NE-SW extension along a SW-dipping, gentle detachment zone was responsible for formation of the core complex. Intrusion of the Hongzhen granite with a biotite plateau age of 124.8±1.2 Ma rendered the ductile shear zone curved, uplifted and final localization of the core complex. The Hongzhen metamorphic core complex suggests that the Early Cretaceous magmatism in this region took place under the condition of regional extension and the eastern Yangtze plate also experienced lithospheric thinning.

  20. Genesis of the Hongzhen metamorphic core complex and its tectonic implications

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The Hongzhen metamorphic core complex is situated in the Yangtze plate to the east of the Dabie oro- genic belt. Its ductile detachment zone in the foot wall overprints on the metamorphic complex of the Proterozoic Dongling Group. The present profile of the ductile shear zone with consistent SW-dipping mineral elongation lineation shows antiform and reversed S-shape from northeast to southwest respectively. Exposure structures, microstructures and quartz C-axis fabric all indicate top-to-SW movement for the ductile shear zone. Recrystallisation types of quartz and feldspar in the mylonites demonstrate that the shear zone was developed under the amphibolite facies condition and at mid-crust levels. The metamorphic core complex formed in the Early Cretaceous with a muscovite plateau age of 124.8±1.2 Ma. Regional NE-SW extension along a SW-dipping, gentle detachment zone was responsible for formation of the core complex. Intrusion of the Hongzhen granite with a biotite plateau age of 124.8±1.2 Ma rendered the ductile shear zone curved, uplifted and final localization of the core complex. The Hongzhen metamorphic core complex suggests that the Early Cretaceous magma- tism in this region took place under the condition of regional extension and the eastern Yangtze plate also experienced lithospheric thinning.

  1. Challenging the Research Base of the Common Core State Standards: A Historical Reanalysis of Text Complexity

    Science.gov (United States)

    Gamson, David A.; Lu, Xiaofei; Eckert, Sarah Anne

    2013-01-01

    The widely adopted Common Core State Standards (CCSS) call for raising the level of text complexity in textbooks and reading materials used by students across all grade levels in the United States; the authors of the English Language Arts component of the CCSS build their case for higher complexity in part upon a research base they say shows a…

  2. Geologic, structural, and thermochronologic constraints on the tectonic evolution of the Sierra Mazatán core complex, Sonora, Mexico: New insights into metamorphic core complex formation

    Science.gov (United States)

    Wong, Martin S.; Gans, Phillip B.

    2008-08-01

    The Sierra Mazatán in northwestern Mexico is the southernmost metamorphic core complex in the North American Cordillera. Large-magnitude Tertiary extension at Sierra Mazatán involved both ductile and brittle slip along a major normal fault that presently dips 10°-15° west. Extension was polyphase and involved an early period of extension from 25 to 23 Ma followed by major slip from 21 to 16 Ma. Total slip was ≤20 km and occurred at rates of 3-4 mm/a. This extension predated the plate boundary change to transtension at ˜12 Ma and was largely decoupled from relative Pacific-North American plate motion. Numerous lines of evidence suggest that the presently low-angle normal fault initiated at a steep dip (50°-60°) and was rotated to lower angles during slip. When corrected for this tilting, fault corrugations at Sierra Mazatán had a similar geometry to the segmentation of many active normal faults, which is compatible with their origin as primary fault features. Many aspects of the Sierra Mazatán are comparable to large active normal faults, indicating that this core complex formed owing to prolonged extension on an otherwise typical high-angle normal fault. Therefore, core complexes need not represent a fundamentally unique mode of crustal extension.

  3. Hot metamorphic core complex in a cold foreland

    Science.gov (United States)

    Franke, Wolfgang; Doublier, Michael Patrick; Klama, Kai; Potel, Sébastien; Wemmer, Klaus

    2011-06-01

    The Montagne Noire forms the southernmost part of the French Massif Central. Carboniferous flysch sediments and very low-grade metamorphic imprint testify to a very external position in the orogen. Sedimentation of synorogenic clastic sediments continued up to the Viséan/Namurian boundary (≤320 Ma). Subsequently, the Palaeozoic sedimentary pile underwent recumbent folding and grossly southward thrusting. An extensional window exposes a hot core of Carboniferous HT/LP gneisses, migmatites and granites (Zone Axiale), which was uplifted from under the nappe pile. After the emplacement of the nappes on the Zone Axiale (Variscan D1), all structural levels shared the same tectonic evolution: D2 (extension and exhumation), D3 (refolding) and post-D3 dextral transtension. HT/LP-metamorphism in the crystalline rocks probably started before and continued after the emplacement of the nappes. Peak metamorphic temperatures were attained during a post-nappe thermal increment (M2). M2 occurred during ENE-directed bilateral extension, which exhumed the Zone Axiale and its frame as a ductile horst structure, flanked to the ENE by a Stephanian intra-montane basin. Map patterns and mesoscopic structures reveal that extension in ENE occurred simultaneously with NNW-oriented shortening. Combination of these D2 effects defines a bulk prolate strain in a "pinched pull-apart" setting. Ductile D2 deformation during M2 dominates the structural record. In wide parts of the nappes on the southern flank of the Zone Axiale, D1 is only represented by the inverted position of bedding (overturned limbs of recumbent D1 folds) and by refolded D1 folds. U-Pb monazite and zircon ages and K-Ar muscovite ages are in accord with Ar-Ar data from the literature. HT/LP metamorphism and granitoid intrusion commenced already at ≥330 Ma and continued until 297 Ma, and probably in a separate pulse in post-Stephanian time. Metamorphic ages older than c. 300 Ma are not compatible with the classical model of

  4. Discovery of protein complexes with core-attachment structures from Tandem Affinity Purification (TAP) data.

    Science.gov (United States)

    Wu, Min; Li, Xiao-Li; Kwoh, Chee-Keong; Ng, See-Kiong; Wong, Limsoon

    2012-09-01

    Many cellular functions involve protein complexes that are formed by multiple interacting proteins. Tandem Affinity Purification (TAP) is a popular experimental method for detecting such multi-protein interactions. However, current computational methods that predict protein complexes from TAP data require converting the co-complex relationships in TAP data into binary interactions. The resulting pairwise protein-protein interaction (PPI) network is then mined for densely connected regions that are identified as putative protein complexes. Converting the TAP data into PPI data not only introduces errors but also loses useful information about the underlying multi-protein relationships that can be exploited to detect the internal organization (i.e., core-attachment structures) of protein complexes. In this article, we propose a method called CACHET that detects protein complexes with Core-AttaCHment structures directly from bipartitETAP data. CACHET models the TAP data as a bipartite graph in which the two vertex sets are the baits and the preys, respectively. The edges between the two vertex sets represent bait-prey relationships. CACHET first focuses on detecting high-quality protein-complex cores from the bipartite graph. To minimize the effects of false positive interactions, the bait-prey relationships are indexed with reliability scores. Only non-redundant, reliable bicliques computed from the TAP bipartite graph are regarded as protein-complex cores. CACHET constructs protein complexes by including attachment proteins into the cores. We applied CACHET on large-scale TAP datasets and found that CACHET outperformed existing methods in terms of prediction accuracy (i.e., F-measure and functional homogeneity of predicted complexes). In addition, the protein complexes predicted by CACHET are equipped with core-attachment structures that provide useful biological insights into the inherent functional organization of protein complexes. Our supplementary material can

  5. Meta-Review of CSF core biomarkers in Alzheimer’s disease: the state-of-the-art after the new revised diagnostic criteria

    Directory of Open Access Journals (Sweden)

    Daniel eFerreira

    2014-03-01

    Full Text Available Background: Current research criteria for Alzheimer’s disease (AD include Cerebrospinal Fluid (CSF biomarkers into the diagnostic algorithm. However, spreading their use to the clinical routine is still questionable. Objective: To provide an updated, systematic and critical review on the diagnostic utility of the CSF core biomarkers for AD. Data sources: MEDLINE, PreMedline, EMBASE, PsycInfo, CINAHL, Cochrane Library, and CRD.Eligibility criteria: 1a Systematic reviews with meta-analysis; 1b Primary studies published after the new revised diagnostic criteria; 2 Evaluation of the diagnostic performance of at least one CSF core biomarker.Results: The diagnostic performance of CSF biomarkers is generally satisfactory. They are optimal for discriminating AD patients from healthy controls. Their combination may also be suitable for Mild Cognitive Impairment (MCI prognosis. However, CSF biomarkers fail to distinguish AD from other forms of dementia. Limitations: 1 use of clinical diagnosis as standard instead of pathological postmortem confirmation; 2 variability of methodological aspects; 3 insufficiently long follow-up periods in MCI studies; and 4 lower diagnostic accuracy in primary care compared with memory clinics. Conclusions: Additional work needs to be done to validate the application of CSF core biomarkers as they are proposed in the new revised diagnostic criteria. The use of CSF core biomarkers in clinical routine is more likely if these limitations are overcome. Early diagnosis is going to be of utmost importance when effective pharmacological treatment will be available and the CSF core biomarkers can also be implemented in clinical trials for drug development.

  6. Meta-Review of CSF Core Biomarkers in Alzheimer’s Disease: The State-of-the-Art after the New Revised Diagnostic Criteria

    Science.gov (United States)

    Ferreira, Daniel; Perestelo-Pérez, Lilisbeth; Westman, Eric; Wahlund, Lars-Olof; Sarría, Antonio; Serrano-Aguilar, Pedro

    2014-01-01

    Background: Current research criteria for Alzheimer’s disease (AD) include cerebrospinal fluid (CSF) biomarkers into the diagnostic algorithm. However, spreading their use to the clinical routine is still questionable. Objective: To provide an updated, systematic and critical review on the diagnostic utility of the CSF core biomarkers for AD. Data sources: MEDLINE, PreMedline, EMBASE, PsycInfo, CINAHL, Cochrane Library, and CRD. Eligibility criteria: (1a) Systematic reviews with meta-analysis; (1b) Primary studies published after the new revised diagnostic criteria; (2) Evaluation of the diagnostic performance of at least one CSF core biomarker. Results: The diagnostic performance of CSF biomarkers is generally satisfactory. They are optimal for discriminating AD patients from healthy controls. Their combination may also be suitable for mild cognitive impairment (MCI) prognosis. However, CSF biomarkers fail to distinguish AD from other forms of dementia. Limitations: (1) Use of clinical diagnosis as standard instead of pathological postmortem confirmation; (2) variability of methodological aspects; (3) insufficiently long follow-up periods in MCI studies; and (4) lower diagnostic accuracy in primary care compared with memory clinics. Conclusion: Additional work needs to be done to validate the application of CSF core biomarkers as they are proposed in the new revised diagnostic criteria. The use of CSF core biomarkers in clinical routine is more likely if these limitations are overcome. Early diagnosis is going to be of utmost importance when effective pharmacological treatment will be available and the CSF core biomarkers can also be implemented in clinical trials for drug development. PMID:24715863

  7. Grafted block complex coacervate core micelles and their effect on protein adsorption on silica and polystyrene

    NARCIS (Netherlands)

    Brzozowska, Agata M.; de Keizer, Arie; Norde, Willem; Detrembleur, Christophe; Stuart, Martien Cohen

    2010-01-01

    We have studied the formation and the stability of grafted block complex coacervate core micelles (C3Ms) in solution and the influence of grafted block C3M coatings on the adsorption of the proteins beta-lactoglobulin, bovine serum albumin, and lysozyme. The C3Ms consist of a grafted block copolymer

  8. Core Self-Evaluations as Causes of Satisfaction: The Mediating Role of Seeking Task Complexity

    Science.gov (United States)

    Srivastava, Abhishek; Locke, Edwin A.; Judge, Timothy A.; Adams, John W.

    2010-01-01

    This study examined the mediating role of task complexity in the relationship between core self-evaluations (CSE) and satisfaction. In Study 1, eighty three undergraduate business students worked on a strategic decision-making simulation. The simulated environment enabled us to verify the temporal sequence of variables, use an objective measure of…

  9. Core Self-Evaluations as Causes of Satisfaction: The Mediating Role of Seeking Task Complexity

    Science.gov (United States)

    Srivastava, Abhishek; Locke, Edwin A.; Judge, Timothy A.; Adams, John W.

    2010-01-01

    This study examined the mediating role of task complexity in the relationship between core self-evaluations (CSE) and satisfaction. In Study 1, eighty three undergraduate business students worked on a strategic decision-making simulation. The simulated environment enabled us to verify the temporal sequence of variables, use an objective measure of…

  10. Complex decay patterns in atomic core photoionization disentangled by ion-recoil measurements

    Energy Technology Data Exchange (ETDEWEB)

    Guillemin, Renaud; Bomme, Cedric; Marin, Thierry; Journel, Loic; Marchenko, Tatiana; Kushawaha, Rajesh K.; Piancastelli, Maria Novella; Simon, Marc [Universite Pierre et Marie Curie, Universite Paris 06, Laboratoire de Chimie Physique Matiere et Rayonement, 11 rue Pierre et Marie Curie, FR-75231 Paris Cedex 05 (France); Centre National de la Recherche Scientifique, Laboratoire de Chimie Physique Matiere et Rayonement (UMR7614), 11 rue Pierre et Marie Curie, FR-75231 Paris Cedex 05 (France); Trcera, Nicolas [Synchrotron SOLEIL, l' Orme des Merisiers, Saint-Aubin, BP 48, FR-91192 Gif-sur-Yvette Cedex (France)

    2011-12-15

    Following core 1s ionization and resonant excitation of argon atoms, we measure the recoil energy of the ions due to momentum conservation during the emission of Auger electrons. We show that such ion momentum spectroscopy can be used to disentangle to some degree complex decay patterns, involving both radiative and nonradiative decays.

  11. Faulting evidence of isostatic uplift in the Rincon Mountains metamorphic core complex: An image processing analysis

    Science.gov (United States)

    Rodriguez-Guerra, Edna Patricia

    This study focuses on the applications of remote sensing techniques and digital analysis to characterizing of tectonic features of the Rincon Mountains metamorphic core complex. Data included Landsat Thematic Mapper (TM) images, digital elevation models (DEM), and digital orthophoto quadrangle quads (DOQQ). The main findings in this study are two nearly orthogonal systems of structures that have never been reported in the Rincon Mountains. The first system, a penetrative faulting system of the footwall rocks, trends N10--30°W. Similar structures identified in other metamorphic core complexes. The second system trends N60--70°E, and has only been alluded indirectly in the literature of metamorphic core complexes. The structures pervade mylonites in Tanque Verde Mountain, Mica Mountain, and the Rincon Peak area. As measured on the imagery, spacing between the N10--30°W lineaments ranges from ˜0.5 to 2 km, and from 0.25 to 1 km for the N60--70°E system. Field inspection reveals that the N10--30°W trending system, are high-angle normal faults dipping mainly to the west. One of the main faults, named here the Cabeza de Vaca fault, has a polished, planar, striated and grooved surface with slickenlines indicating pure normal dip-slip movement (N10°W, 83°SW; slickensides rake 85°SW). The Cabeza de Vaca fault is the eastern boundary of a 2 km-wide graben, with displacement as great as 400 meters. The N10--30°W faults are syn- to post-mylonitic, high-angle normal faults that formed during isostatic uplift of the Rincon core complex during mid-Tertiary time. This interpretation is based on previous works, which report similar fault patterns in other metamorphic core complexes. Faults trending N20--30°W, shape the east flank of Mica Mountain. These faults, on the back dipping mylonitic zone, dip east and may represent late-stage antithetic shear zones. The Cabeza de Vaca fault and the back dipping antithetic faults accommodate as much as 65% of the extension due to

  12. CORE

    DEFF Research Database (Denmark)

    Krigslund, Jeppe; Hansen, Jonas; Hundebøll, Martin

    2013-01-01

    different flows. Instead of maintaining these approaches separate, we propose a protocol (CORE) that brings together these coding mechanisms. Our protocol uses random linear network coding (RLNC) for intra- session coding but allows nodes in the network to setup inter- session coding regions where flows...... intersect. Routes for unicast sessions are agnostic to other sessions and setup beforehand, CORE will then discover and exploit intersecting routes. Our approach allows the inter-session regions to leverage RLNC to compensate for losses or failures in the overhearing or transmitting process. Thus, we...... increase the benefits of XORing by exploiting the underlying RLNC structure of individual flows. This goes beyond providing additional reliability to each individual session and beyond exploiting coding opportunistically. Our numerical results show that CORE outperforms both forwarding and COPE...

  13. CORE

    DEFF Research Database (Denmark)

    Krigslund, Jeppe; Hansen, Jonas; Hundebøll, Martin

    2013-01-01

    different flows. Instead of maintaining these approaches separate, we propose a protocol (CORE) that brings together these coding mechanisms. Our protocol uses random linear network coding (RLNC) for intra- session coding but allows nodes in the network to setup inter- session coding regions where flows...... intersect. Routes for unicast sessions are agnostic to other sessions and setup beforehand, CORE will then discover and exploit intersecting routes. Our approach allows the inter-session regions to leverage RLNC to compensate for losses or failures in the overhearing or transmitting process. Thus, we...... increase the benefits of XORing by exploiting the underlying RLNC structure of individual flows. This goes beyond providing additional reliability to each individual session and beyond exploiting coding opportunistically. Our numerical results show that CORE outperforms both forwarding and COPE...

  14. Hydrophilic drug encapsulation in shell-core microcarriers by two stage polyelectrolyte complexation method.

    Science.gov (United States)

    Dalmoro, Annalisa; Sitenkov, Alexander Y; Cascone, Sara; Lamberti, Gaetano; Barba, Anna Angela; Moustafine, Rouslan I

    2017-02-25

    In this study a protocol exploiting the combination of the ultrasonic atomization and the complexation between polyelectrolytes was developed to efficiently encapsulate a hydrophilic chemotherapeutic agent essentially used in the treatment of colon cancer, 5-fluorouracil, in enteric shell-core alginate-based microcarriers. The atomization assisted by ultrasound allowed to obtain small droplets by supplying low energy and avoiding drug degradation. In particular microcarriers were produced in a home-made apparatus where both the core (composed of alginate, drug, and Pluronic F127) and shell (composed of only alginate) feed were separately sent to the coaxial ultrasonic atomizer where they were nebulized and placed in contact with the complexation bulk. With the aim to obtain microstructured particles of alginate encapsulating 5-fluorouracil, different formulations of the first complexation bulk were tested; at last an emulsion made of a calcium chloride aqueous solution and dichloromethane allowed to reach an encapsulation efficiency of about 50%. This result can be considered very interesting considering that in literature similar techniques gave 5-fluorouracil encapsulation efficiencies of about 10%. Since a single complexation stage was not able to assure microcarriers gastroresistance, the formulation of a second complexation bulk was evaluated. The solution of cationic and pH-insoluble Eudragit® RS 100 in dichloromethane was chosen as bulk of second-stage complexation obtaining good enteric properties of shell-core microcarriers, i.e. a 5-FU cumulative release at pH 1 (simulating gastric pH) lower than 35%. The formation of interpolyelectrolyte complex (IPEC) between countercharged polymers and the chemical stability of 5-FU in microcarriers were confirmed by FTIR analysis, the presence of an amorphous dispersion of 5-FU in prepared microparticles was also confirmed by DSC. Finally, shell-core enteric coated microcarriers encapsulating 5-fluorouracil were used

  15. The role of partial melting and extensional strain rates in the development of metamorphic core complexes

    Science.gov (United States)

    Rey, P. F.; Teyssier, C.; Whitney, D. L.

    2009-11-01

    Orogenic collapse involves extension and thinning of thick and hot (partially molten) crust, leading to the formation of metamorphic core complexes (MCC) that are commonly cored by migmatite domes. Two-dimensional thermo-mechanical Ellipsis models evaluate the parameters that likely control the formation and evolution of MCC: the nature and geometry of the heterogeneity that localizes MCC, the presence/absence of a partially molten layer in the lower crust, and the rate of extension. When the localizing heterogeneity is a normal fault in the upper crust, the migmatite core remains in the footwall of the fault, resulting in an asymmetric MCC; if the localizing heterogeneity is point like region within the upper crust, the MCC remains symmetric throughout its development. Therefore, asymmetrically located migmatite domes likely reflect the dip of the original normal fault system that generated the MCC. Modeling of a severe viscosity drop owing to the presence of a partially molten layer, compared to a crust with no melt, demonstrates that the presence of melt slightly enhances upward advection of material and heat. Our experiments show that, when associated with boundary-driven extension, far-field horizontal extension provides space for the domes. Therefore, the buoyancy of migmatite cores contributes little to the outer envelope of metamorphic core complexes, although it may play a significant role in the internal dynamics of the partially molten layer. The presence of melt also favors heterogeneous bulk pure shear of the dome as opposed to the bulk simple shear, which dominates in melt-absent experiments. Melt presence affects the shape of P-T-t paths only slightly for material located near the top of the low-viscosity layer but leads to more complex flow paths for material inside the layer. The effect of extension rate is significant: at high extension rate (cm yr - 1 in the core complex region), partially molten crust crystallizes and cools along a high

  16. Cryo-EM Structure of the TOM Core Complex from Neurospora crassa.

    Science.gov (United States)

    Bausewein, Thomas; Mills, Deryck J; Langer, Julian D; Nitschke, Beate; Nussberger, Stephan; Kühlbrandt, Werner

    2017-08-10

    The TOM complex is the main entry gate for protein precursors from the cytosol into mitochondria. We have determined the structure of the TOM core complex by cryoelectron microscopy (cryo-EM). The complex is a 148 kDa symmetrical dimer of ten membrane protein subunits that create a shallow funnel on the cytoplasmic membrane surface. In the core of the dimer, the β-barrels of the Tom40 pore form two identical preprotein conduits. Each Tom40 pore is surrounded by the transmembrane segments of the α-helical subunits Tom5, Tom6, and Tom7. Tom22, the central preprotein receptor, connects the two Tom40 pores at the dimer interface. Our structure offers detailed insights into the molecular architecture of the mitochondrial preprotein import machinery. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. The Spatial Distribution of Complex Organic Molecules in the L1544 Pre-stellar Core

    OpenAIRE

    2016-01-01

    The detection of complex organic molecules (COMs) toward cold sources such as pre-stellar cores (with T=30 mag within the inner 2700 au; and a low-density shell with average Av~7.5-8 mag located at 4000 au from the core's center and bright in CH3OH. Our observations show that CH3O, CH3OCH3 and CH3CHO are more abundant (by factors ~2-10) toward the low-density shell than toward the continuum peak. Other COMs such as CH3OCHO, c-C3H2O, HCCCHO, CH2CHCN and HCCNC show slight enhancements (by factors

  18. Construction of the energy matrix for complex atoms. Part VI: Core polarization effects

    Science.gov (United States)

    Elantkowska, Magdalena; Ruczkowski, Jarosław; Dembczyński, Jerzy

    2016-12-01

    The continuation of series of papers concerning the construction of the energy matrix for complex atoms is presented. The second-order perturbation theory contributions originating from core polarization effects in the hyperfine structure are considered. Fifteen new formulae for angular coefficients of core polarization parameters are given. The complete set of corrections up to the second-order perturbation theory was taken into account and the accuracy of the wave functions in the intermediate coupling scheme, on the example of the lanthanum atom, was checked.

  19. FANCI Regulates Recruitment of the FA Core Complex at Sites of DNA Damage Independently of FANCD2.

    Directory of Open Access Journals (Sweden)

    Maria Castella

    2015-10-01

    Full Text Available The Fanconi anemia (FA-BRCA pathway mediates repair of DNA interstrand crosslinks. The FA core complex, a multi-subunit ubiquitin ligase, participates in the detection of DNA lesions and monoubiquitinates two downstream FA proteins, FANCD2 and FANCI (or the ID complex. However, the regulation of the FA core complex itself is poorly understood. Here we show that the FA core complex proteins are recruited to sites of DNA damage and form nuclear foci in S and G2 phases of the cell cycle. ATR kinase activity, an intact FA core complex and FANCM-FAAP24 were crucial for this recruitment. Surprisingly, FANCI, but not its partner FANCD2, was needed for efficient FA core complex foci formation. Monoubiquitination or ATR-dependent phosphorylation of FANCI were not required for the FA core complex recruitment, but FANCI deubiquitination by USP1 was. Additionally, BRCA1 was required for efficient FA core complex foci formation. These findings indicate that FANCI functions upstream of FA core complex recruitment independently of FANCD2, and alter the current view of the FA-BRCA pathway.

  20. Cenozoic crustal extension in southeastern Arizona and implications for models of core-complex development

    Science.gov (United States)

    Arca, M. Serkan; Kapp, Paul; Johnson, Roy A.

    2010-06-01

    In conventional models of Cordilleran-style metamorphic core-complex development, initial extension occurs along a breakaway fault, which subsequently is deformed into a synform and abandoned in response to isostatic rebound and new faults breaking forward in the dominant transport direction. The Catalina core complex and associated geology in southeastern Arizona have been pointed to as a type example of this model. From southwest to northeast, the region is characterized by the NW-SE trending Tucson basin, the Catalina core complex, the San Pedro trough and the Galiuro Mountains. The Catalina core complex is bounded by the top-to-the-southwest Catalina detachment fault along its southwestern flank and the low-angle, northeast-dipping San Pedro fault along its northeastern flank. The Galiuro Mountains expose non-mylonitic rocks and are separated from the San Pedro trough to the southwest by a system of low- to moderate-angle southwest-dipping normal faults. This Galiuro fault system is widely interpreted to be the breakaway zone for the Catalina core complex. It is inferred to be folded into a synform beneath the San Pedro trough, to resurface to the southwest as the San Pedro fault, and to have been abandoned during slip along the younger Catalina detachment. This study aimed to test this model through analysis of field relations and geochronological age constraints, and reprocessing and interpretation of 2-D seismic reflection data from the Catalina core complex and San Pedro trough. In contrast to predictions of the conventional breakaway zone model, we raise the possibility of a moderate-angle, southwest-dipping detachment fault beneath the San Pedro trough that could extend to mid-crustal depths beneath the eastern flank of the Catalina Mountains. We present an alternative kinematic model in which extension was accommodated by a pair of top-to-the-southwest normal-fault systems (the Catalina and Galiuro detachment faults), with the only major difference

  1. Organic light-emitting diodes incorporating nanometer thick films of europium-cored complexes

    Science.gov (United States)

    Phelan, Gregory D.; Carlson, Brenden; Jiang, Xuezhong; Jen, Alex K. Y.; Dalton, Larry R.

    2002-11-01

    Europium cored complexes may be used as a source of red emission in light emitting diodes. Novel europium cored complexes have been synthesized and incorporated into organic light emitting diodes (OLED's). These complexes emit red light at 615 nm with a full width half maximum (FWHM) of less than 5 nm. The europium complexes consist of one equivalent of europium chelated to three equivalents of a nonsymmetrical β-diketone ligand. The Claissen condensation of a polycyclic aromatic sensitizer and an ester of a fluorinated carboxylic acid create the ligands. The use of a sensitizer such as phenanthrene results in a ligand that has an emission band that directly overlaps with the absorption band of europium. The use of fluorinated chains improves the overall processibility as well as the charge transfer capability of the resulting metal cored complex. The europium core is further encapsulated by the inclusion of an additional polycyclic aromatic compound such as 4, 7 diphenyl - 1, 10 phenanthroline. Emission of 615 nm light is accomplished through excitation of the ligand and efficient Forrester energy transfer to the europium complex. A multiple layer device consisting of a substrate of indium tin oxide, followed by thin layers of BTPD-PFCB (with a thickness of 20nm), a polymer blend containing the europium complex (30 nm), followed by a layer of calcium (50nm) and finally a protective layer of silver (120 nm). The polymer blends were either poly(n-vinyl carbazole)(PVK) or poly vinyl naphthalene (PVN). The device performance was further improved by the incorporation of another lanthanide metal complex. These complexes were based upon similar ligands surrounding gadolinium. In these devices, there is a Dexter energy transfer as well as the Forster energy transfer. For the devices that are based on a PVN:PBD as a polymer host, the lowest turn on voltage was 12.0 volts. The devices that use PVK:TPD devices was 178 cd/m2 with an external quantum efficiency of 0.61%.For

  2. Structure and Cellular Roles of the RMI Core Complex from the Bloom Syndrome Dissolvasome

    Energy Technology Data Exchange (ETDEWEB)

    Hoadley, Kelly A.; Xu, Dongyi; Xue, Yutong; Satyshur, Kenneth A.; Wang, Weidong; Keck, James L. (NIH); (UW-MED)

    2010-11-11

    BLM, the protein product of the gene mutated in Bloom syndrome, is one of five human RecQ helicases. It functions to separate double Holliday junction DNA without genetic exchange as a component of the dissolvasome, which also includes topoisomerase III{alpha} and the RMI (RecQ-mediated genome instability) subcomplex (RMI1 and RMI2). We describe the crystal structure of the RMI core complex, comprising RMI2 and the C-terminal OB domain of RMI1. The overall RMI core structure strongly resembles two-thirds of the trimerization core of the eukaryotic single-stranded DNA-binding protein, Replication Protein A. Immunoprecipitation experiments with RMI2 variants confirm key interactions that stabilize the RMI core interface. Disruption of this interface leads to a dramatic increase in cellular sister chromatid exchange events similar to that seen in BLM-deficient cells. The RMI core interface is therefore crucial for BLM dissolvasome assembly and may have additional cellular roles as a docking hub for other proteins.

  3. Structure and cellular roles of the RMI core complex from the bloom syndrome dissolvasome.

    Science.gov (United States)

    Hoadley, Kelly A; Xu, Dongyi; Xue, Yutong; Satyshur, Kenneth A; Wang, Weidong; Keck, James L

    2010-09-08

    BLM, the protein product of the gene mutated in Bloom syndrome, is one of five human RecQ helicases. It functions to separate double Holliday junction DNA without genetic exchange as a component of the "dissolvasome," which also includes topoisomerase IIIα and the RMI (RecQ-mediated genome instability) subcomplex (RMI1 and RMI2). We describe the crystal structure of the RMI core complex, comprising RMI2 and the C-terminal OB domain of RMI1. The overall RMI core structure strongly resembles two-thirds of the trimerization core of the eukaryotic single-stranded DNA-binding protein, Replication Protein A. Immunoprecipitation experiments with RMI2 variants confirm key interactions that stabilize the RMI core interface. Disruption of this interface leads to a dramatic increase in cellular sister chromatid exchange events similar to that seen in BLM-deficient cells. The RMI core interface is therefore crucial for BLM dissolvasome assembly and may have additional cellular roles as a docking hub for other proteins.

  4. Strategies for crystallizing a chromatin protein in complex with the nucleosome core particle.

    Science.gov (United States)

    Makde, Ravindra D; Tan, Song

    2013-11-15

    The molecular details of how chromatin factors and enzymes interact with the nucleosome are critical to understanding fundamental genetic processes including cell division and gene regulation. A structural understanding of such processes has been hindered by the difficulty in producing diffraction-quality crystals of chromatin proteins in complex with the nucleosome. We describe here the steps used to grow crystals of the 300-kDa RCC1 chromatin factor/nucleosome core particle complex that diffract to 2.9-Å resolution. These steps include both pre- and postcrystallization strategies potentially useful to other complexes. We screened multiple variant RCC1/nucleosome core particle complexes assembled using different RCC1 homologs and deletion variants, and nucleosomes containing nucleosomal DNA with different sequences and lengths, as well as histone deletion variants. We found that using RCC1 from different species produced different crystal forms of the RCC1/nucleosome complex consistent with key crystal packing interactions mediated by RCC1. Optimization of postcrystallization soaks to dehydrate the crystals dramatically improved the diffraction quality of the RCC1/nucleosome crystal from 5.0- to 2.9-Å resolution. Copyright © 2013 Elsevier Inc. All rights reserved.

  5. Simulation Based Optimization of Complex Monolithic Composite Structures Using Cellular Core Technology

    Science.gov (United States)

    Hickmott, Curtis W.

    Cellular core tooling is a new technology which has the capability to manufacture complex integrated monolithic composite structures. This novel tooling method utilizes thermoplastic cellular cores as inner tooling. The semi-rigid nature of the cellular cores makes them convenient for lay-up, and under autoclave temperature and pressure they soften and expand providing uniform compaction on all surfaces including internal features such as ribs and spar tubes. This process has the capability of developing fully optimized aerospace structures by reducing or eliminating assembly using fasteners or bonded joints. The technology is studied in the context of evaluating its capabilities, advantages, and limitations in developing high quality structures. The complex nature of these parts has led to development of a model using the Finite Element Analysis (FEA) software Abaqus and the plug-in COMPRO Common Component Architecture (CCA) provided by Convergent Manufacturing Technologies. This model utilizes a "virtual autoclave" technique to simulate temperature profiles, resin flow paths, and ultimately deformation from residual stress. A model has been developed simulating the temperature profile during curing of composite parts made with the cellular core technology. While modeling of composites has been performed in the past, this project will look to take this existing knowledge and apply it to this new manufacturing method capable of building more complex parts and develop a model designed specifically for building large, complex components with a high degree of accuracy. The model development has been carried out in conjunction with experimental validation. A double box beam structure was chosen for analysis to determine the effects of the technology on internal ribs and joints. Double box beams were manufactured and sectioned into T-joints for characterization. Mechanical behavior of T-joints was performed using the T-joint pull-off test and compared to traditional

  6. Core size determination and structural characterization of intravenous iron complexes by cryogenic transmission electron microscopy.

    Science.gov (United States)

    Wu, Yong; Petrochenko, Peter; Chen, Lynn; Wong, Sook Yee; Absar, Mohammad; Choi, Stephanie; Zheng, Jiwen

    2016-05-30

    Understanding physicochemical properties of intravenous (IV) iron drug products is essential to ensure the manufacturing process is consistent and streamlined. The history of physicochemical characterization of IV iron complex formulations stretches over several decades, with disparities in iron core size and particle morphology as the major source of debate. One of the main reasons for this controversy is room temperature sample preparation artifacts, which affect accurate determination of size, shape and agglomeration/aggregation of nanoscale iron particles. The present study is first to report the ultra-fine iron core structures of four IV iron complex formulations, sodium ferric gluconate, iron sucrose, low molecular weight iron dextran and ferumoxytol, using a cryogenic transmission electron microscopy (cryo-TEM) preservation technique, as opposed to the conventional room temperature (RT-TEM) technique. Our results show that room temperature preparation causes nanoparticle aggregation and deformation, while cryo-TEM preserves IV iron colloidal suspension in their native frozen-hydrated and undiluted state. In contrast to the current consensus in literature, all four IV iron colloids exhibit a similar morphology of their iron oxide cores with a spherical shape, narrow size distribution and an average size of 2nm. Moreover, out of the four tested formulations, ferumoxytol exhibits a cluster-like community of several iron carbohydrate particles which likely accounts for its large hydrodynamic size of 25nm, measured with dynamic light scattering. Our findings outline a suitable method for identifying colloidal nanoparticle core size in the native state, which is increasingly important for manufacturing and design control of complex drug formulations, such as IV iron drug products.

  7. Temperature responsive complex coacervate core micelles with a PEO and PNIPAAm corona.

    Science.gov (United States)

    Voets, Ilja K; Moll, Puck M; Aqil, Abdelhafid; Jérôme, Christine; Detrembleur, Christophe; Waard, Pieter de; Keizer, Arie de; Stuart, Martien A Cohen

    2008-09-01

    In aqueous solutions at room temperature, poly( N-methyl-2-vinyl pyridinium iodide)- block-poly(ethylene oxide), P2MVP 38- b-PEO 211 and poly(acrylic acid)- block-poly(isopropyl acrylamide), PAA 55- b-PNIPAAm 88 spontaneously coassemble into micelles, consisting of a mixed P2MVP/PAA polyelectrolyte core and a PEO/PNIPAAm corona. These so-called complex coacervate core micelles (C3Ms), also known as polyion complex (PIC) micelles, block ionomer complexes (BIC), and interpolyelectrolyte complexes (IPEC), respond to changes in solution pH and ionic strength as their micellization is electrostatically driven. Furthermore, the PNIPAAm segments ensure temperature responsiveness as they exhibit lower critical solution temperature (LCST) behavior. Light scattering, two-dimensional 1H NMR nuclear Overhauser effect spectrometry, and cryogenic transmission electron microscopy experiments were carried out to investigate micellar structure and solution behavior at 1 mM NaNO 3, T = 25, and 60 degrees C, that is, below and above the LCST of approximately 32 degrees C. At T = 25 degrees C, C3Ms were observed for 7 coacervate shell, and a PEO corona.

  8. Hyperbranched red light-emitting phosphorescent polymers based on iridium complex as the core

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Ting; Yu, Lei; Yang, Yong; Li, Yanhu; Tao, Yun [Institute of Polymer Optoelectronic Materials and Devices, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640 (China); Hou, Qiong [School of Chemistry & Environment, South China Normal University, Guangzhou 510006 (China); Ying, Lei, E-mail: msleiying@scut.edu.cn [Institute of Polymer Optoelectronic Materials and Devices, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640 (China); Yang, Wei; Wu, Hongbin; Cao, Yong [Institute of Polymer Optoelectronic Materials and Devices, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640 (China)

    2015-11-15

    A series of hyperbranched π-conjugated light-emitting polymers containing an iridium complex as the branched core unit and polyfluorene or poly(fluorene-alt-carbazole) as the branched segments were synthesized via a palladium catalyzed Suzuki polymerization. Apparent Förster energy transfer in the photoluminescent spectra as thin films was observed, while no discernible characteristic absorbance and photoluminescence of the iridium complex can be realized in dilute solutions. Copolymers based on poly(fluorene-alt-carbazole) as the branched segments demonstrated enhanced highest occupied molecular orbital energy levels relative to those based on polyfluorene. The electroluminescent spectra of these copolymers exclusively showed the characteristic emission of the iridium complex, with corresponding CIE coordinates of (0.67±0.01, 0.31). All devices exhibited relatively slow roll-off of efficiency, and the best device performance with the maximum luminous efficiency of 5.33 cd A{sup −1} was attained by using PFCzTiqIr3 as the emissive layer. These results indicated that the hyperbranched conjugated architectures can be a promising molecular design strategy for efficient electrophosphorescent light-emitting polymers. - Highlights: • Hyperbranched red light-emitting polymers are synthesized. • Red light-emitting iridium complex is used as the branched core unit. • Hyperbranched polymers based on PFCz exhibit higher luminescence. • The highest luminous efficiency of 5.33 cd A{sup −1} is attained.

  9. IODP Expedition 340T: Borehole Logging at Atlantis Massif Oceanic Core Complex

    Directory of Open Access Journals (Sweden)

    Donna Blackman

    2013-03-01

    Full Text Available Integrated Ocean Drilling Program (IODP Expedition 340T returned to the 1.4-km-deep Hole U1309D at Atlantis Massif to carry out borehole logging including vertical seismic profiling (VSP. Seismic, resistivity, and temperature logs were obtained throughout the geologic section in the footwall of this oceanic core complex. Reliable downhole temperature measurements throughout and the first seismic coverage of the 800–1400 meters below seafloor (mbsf portionof the section were obtained. Distinct changes in velocity, resistivity, and magnetic susceptibility characterize the boundaries of altered, olivine-rich troctolite intervals within the otherwise dominantly gabbroic se-quence. Some narrow fault zones also are associated with downhole resistivity or velocity excursions. Small deviations in temperature were measured in borehole fluid adjacent to known faults at 750 mbsf and 1100 mbsf. This suggests that flow of seawater remains active along these zones of faulting and rock alteration. Vertical seismic profile station coverage at zero offsetnow extends the full length of the hole, including the uppermost 150 mbsf, where detachment processes are expected to have left their strongest imprint. Analysis of wallrock properties, together with alteration and structural characteristics of the cores from Site U1309, highlights the likely interplay between lithology, structure, lithospheric hydration, and core complex evolution.

  10. Major histocompatibility complex class I core promoter elements are not essential for transcription in vivo.

    Science.gov (United States)

    Barbash, Zohar S; Weissman, Jocelyn D; Campbell, John A; Mu, Jie; Singer, Dinah S

    2013-11-01

    The role of core promoter elements in regulating transcription initiation is largely unknown for genes subject to complex regulation. Major histocompatibility complex class I genes are ubiquitously expressed and governed by tissue-specific and hormonal signals. Transcription initiates at multiple sites within the core promoter, which contains elements homologous to the canonical elements CCAAT, TATAA, Sp1 binding site (Sp1BS), and Initiator (Inr). To determine their functions, expression of class I transgenes with individually mutated elements was assessed. Surprisingly, all mutant promoters supported transcription. However, each mutated core promoter element had a distinct effect on expression: CAAT box mutations modulated constitutive expression in nonlymphoid tissues, whereas TATAA-like element mutations dysregulated transcription in lymphoid tissues. Inr mutations aberrantly elevated expression. Sp1BS element mutations resulted in variegated transgene expression. RNA polymerase II binding and histone H3K4me3 patterns correlated with transgene expression; H3K9me3 marks partially correlated. Whereas the wild-type, TATAA-like, and CAAT mutant promoters were activated by gamma interferon, the Sp1 and Inr mutants were repressed, implicating these elements in regulation of hormonal responses. These results lead to the surprising conclusion that no single element is required for promoter activity. Rather, each plays a distinct role in promoter activity, chromatin structure, tissue-specific expression, and extracellular signaling.

  11. Fanconi anemia core complex gene promoters harbor conserved transcription regulatory elements.

    Directory of Open Access Journals (Sweden)

    Daniel Meier

    Full Text Available The Fanconi anemia (FA gene family is a recent addition to the complex network of proteins that respond to and repair certain types of DNA damage in the human genome. Since little is known about the regulation of this novel group of genes at the DNA level, we characterized the promoters of the eight genes (FANCA, B, C, E, F, G, L and M that compose the FA core complex. The promoters of these genes show the characteristic attributes of housekeeping genes, such as a high GC content and CpG islands, a lack of TATA boxes and a low conservation. The promoters functioned in a monodirectional way and were, in their most active regions, comparable in strength to the SV40 promoter in our reporter plasmids. They were also marked by a distinctive transcriptional start site (TSS. In the 5' region of each promoter, we identified a region that was able to negatively regulate the promoter activity in HeLa and HEK 293 cells in isolation. The central and 3' regions of the promoter sequences harbor binding sites for several common and rare transcription factors, including STAT, SMAD, E2F, AP1 and YY1, which indicates that there may be cross-connections to several established regulatory pathways. Electrophoretic mobility shift assays and siRNA experiments confirmed the shared regulatory responses between the prominent members of the TGF-β and JAK/STAT pathways and members of the FA core complex. Although the promoters are not well conserved, they share region and sequence specific regulatory motifs and transcription factor binding sites (TBFs, and we identified a bi-partite nature to these promoters. These results support a hypothesis based on the co-evolution of the FA core complex genes that was expanded to include their promoters.

  12. Engineering task plan for the annual revision of the rotary mode core sampling system safety equipment list

    Energy Technology Data Exchange (ETDEWEB)

    BOGER, R.M.

    1999-05-13

    This Engineering Task Plan addresses an effort to provide an update to the RMCS Systems 3 and 4 SEL and DCM in order to incorporate the changes to the authorization basis implemented by HNF-SD-WM-BIO-001, Rev. 0 (Draft), Addendum 5 , Safety Analysis for Rotary Mode Core Sampling. Responsibilities, task description, cost estimate, and schedule are presented.

  13. Rheological transitions in the middle crust: insights from Cordilleran metamorphic core complexes

    Science.gov (United States)

    Cooper, Frances J.; Platt, John P.; Behr, Whitney M.

    2017-02-01

    High-strain mylonitic rocks in Cordilleran metamorphic core complexes reflect ductile deformation in the middle crust, but in many examples it is unclear how these mylonites relate to the brittle detachments that overlie them. Field observations, microstructural analyses, and thermobarometric data from the footwalls of three metamorphic core complexes in the Basin and Range Province, USA (the Whipple Mountains, California; the northern Snake Range, Nevada; and Ruby Mountains-East Humboldt Range, Nevada), suggest the presence of two distinct rheological transitions in the middle crust: (1) the brittle-ductile transition (BDT), which depends on thermal gradient and tectonic regime, and marks the switch from discrete brittle faulting and cataclasis to continuous, but still localized, ductile shear, and (2) the localized-distributed transition, or LDT, a deeper, dominantly temperature-dependent transition, which marks the switch from localized ductile shear to distributed ductile flow. In this model, brittle normal faults in the upper crust persist as ductile shear zones below the BDT in the middle crust, and sole into the subhorizontal LDT at greater depths.In metamorphic core complexes, the presence of these two distinct rheological transitions results in the development of two zones of ductile deformation: a relatively narrow zone of high-stress mylonite that is spatially and genetically related to the brittle detachment, underlain by a broader zone of high-strain, relatively low-stress rock that formed in the middle crust below the LDT, and in some cases before the detachment was initiated. The two zones show distinct microstructural assemblages, reflecting different conditions of temperature and stress during deformation, and contain superposed sequences of microstructures reflecting progressive exhumation, cooling, and strain localization. The LDT is not always exhumed, or it may be obscured by later deformation, but in the Whipple Mountains, it can be directly

  14. HCV Core Residues Critical for Infectivity Are Also Involved in Core-NS5A Complex Formation

    Science.gov (United States)

    Gawlik, Katarzyna; Baugh, James; Chatterji, Udayan; Lim, Precious J.; Bobardt, Michael D.; Gallay, Philippe A.

    2014-01-01

    Hepatitis C virus (HCV) infection is a major cause of liver disease. The molecular machinery of HCV assembly and particle release remains obscure. A better understanding of the assembly events might reveal new potential antiviral strategies. It was suggested that the nonstructural protein 5A (NS5A), an attractive recent drug target, participates in the production of infectious particles as a result of its interaction with the HCV core protein. However, prior to the present study, the NS5A-binding site in the viral core remained unknown. We found that the D1 domain of core contains the NS5A-binding site with the strongest interacting capacity in the basic P38-K74 cluster. We also demonstrated that the N-terminal basic residues of core at positions 50, 51, 59 and 62 were required for NS5A binding. Analysis of all substitution combinations of R50A, K51A, R59A, and R62A, in the context of the HCVcc system, showed that single, double, triple, and quadruple mutants were fully competent for viral RNA replication, but deficient in secretion of viral particles. Furthermore, we found that the extracellular and intracellular infectivity of all the mutants was abolished, suggesting a defect in the formation of infectious particles. Importantly, we showed that the interaction between the single and quadruple core mutants and NS5A was impaired in cells expressing full-length HCV genome. Interestingly, mutations of the four basic residues of core did not alter the association of core or NS5A with lipid droplets. This study showed for the first time that basic residues in the D1 domain of core that are critical for the formation of infectious extracellular and intracellular particles also play a role in core-NS5A interactions. PMID:24533158

  15. The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.

    Science.gov (United States)

    Topalidou, Irini; Cattin-Ortolá, Jérôme; Pappas, Andrea L; Cooper, Kirsten; Merrihew, Gennifer E; MacCoss, Michael J; Ailion, Michael

    2016-05-01

    The dense-core vesicle is a secretory organelle that mediates the regulated release of peptide hormones, growth factors, and biogenic amines. Dense-core vesicles originate from the trans-Golgi of neurons and neuroendocrine cells, but it is unclear how this specialized organelle is formed and acquires its specific cargos. To identify proteins that act in dense-core vesicle biogenesis, we performed a forward genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We previously reported the identification of two conserved proteins that interact with the small GTPase RAB-2 to control normal dense-core vesicle cargo-sorting. Here we identify several additional conserved factors important for dense-core vesicle cargo sorting: the WD40 domain protein EIPR-1 and the endosome-associated recycling protein (EARP) complex. By assaying behavior and the trafficking of dense-core vesicle cargos, we show that mutants that lack EIPR-1 or EARP have defects in dense-core vesicle cargo-sorting similar to those of mutants in the RAB-2 pathway. Genetic epistasis data indicate that RAB-2, EIPR-1 and EARP function in a common pathway. In addition, using a proteomic approach in rat insulinoma cells, we show that EIPR-1 physically interacts with the EARP complex. Our data suggest that EIPR-1 is a new interactor of the EARP complex and that dense-core vesicle cargo sorting depends on the EARP-dependent trafficking of cargo through an endosomal sorting compartment.

  16. Light and Heat Induced Denaturation of Photosystem Ⅱ Core Antenna Complex CP47

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Light and heat induced denaturation of CP47, the core antenna complex of photosystem Ⅱ purified from spinach, were investigated using absorption and circular dichroism spectra.Light caused the destruction of chlorophyll a and excitonic interaction of chlorophyll a in CP47, while the protein secondary structure was not apparently changed.Heat induced the destruction of protein secondary structure and excitonic interaction of chlorophyll a, but the chlorophyll a molecule was not damaged.The results suggest that both the chlorophyll a molecular structure and the protein native conformation are necessary for excitonic interaction of chlorophyll a and the energy transfer function of the chlorophyll a binding protein.

  17. Documentation for the NCES Common Core of Data National Public Education Financial Survey (NPEFS), School Year 2008-09 (Fiscal Year 2009). Revised File Version 1b. NCES 2011-330rev

    Science.gov (United States)

    Cornman, Stephen Q.; Zhou, Lei; Nakamoto, Nanae

    2012-01-01

    This documentation is for the revised file (Version 1b) of the National Center for Education Statistics' (NCES) Common Core of Data (CCD) National Public Education Financial Survey (NPEFS) for school year 2008-2009, fiscal year 2009 (FY 09). It contains a brief description of the data collection along with information required to understand and…

  18. A high resolution study of complex organic molecules in hot cores

    CERN Document Server

    Calcutt, Hannah; Codella, Claudio; Beltrán, Maria T; Fontani, Francesco; Woods, Paul M

    2014-01-01

    We present the results of a line identification analysis using data from the IRAM Plateau de Bure Inferferometer, focusing on six massive star-forming hot cores: G31.41+0.31, G29.96-0.02, G19.61-0.23, G10.62-0.38, G24.78+0.08A1 and G24.78+0.08A2. We identify several transitions of vibrationally excited methyl formate (HCOOCH$_3$) for the first time in these objects as well as transitions of other complex molecules, including ethyl cyanide (C$_2$H$_5$CN), and isocyanic acid (HNCO). We also postulate a detection of one transition of glycolaldehyde (CH$_2$(OH)CHO) in two new hot cores. We find G29.96-0.02, G19.61-0.23, G24.78+0.08A1 and 24.78+0.08A2 to be chemically very similar. G31.41+0.31, however, is chemically different: it manifests a larger chemical inventory and has significantly larger column densities. We suggest that it may represent a different evolutionary stage to the other hot cores in the sample, or it may surround a star with a higher mass. We derive column densities for methyl formate in G31.41...

  19. The Spatial Distribution of Complex Organic Molecules in the L1544 Pre-stellar Core

    CERN Document Server

    Jimenez-Serra, Izaskun; Caselli, Paola; Marcelino, Nuria; Billot, Nicolas; Viti, Serena; Testi, Leonardo; Vastel, Charlotte; Lefloch, Bertrand; Bachiller, Rafael

    2016-01-01

    The detection of complex organic molecules (COMs) toward cold sources such as pre-stellar cores (with T=30 mag within the inner 2700 au; and a low-density shell with average Av~7.5-8 mag located at 4000 au from the core's center and bright in CH3OH. Our observations show that CH3O, CH3OCH3 and CH3CHO are more abundant (by factors ~2-10) toward the low-density shell than toward the continuum peak. Other COMs such as CH3OCHO, c-C3H2O, HCCCHO, CH2CHCN and HCCNC show slight enhancements (by factors <=3) but the associated uncertainties are large. This suggests that COMs are actively formed and already present in the low-density shells of pre-stellar cores. The modelling of the chemistry of O-bearing COMs in L1544 indicates that these species are enhanced in this shell because i) CO starts freezing out onto dust grains driving an active surface chemistry; ii) the visual extinction is sufficiently high to prevent the UV photo-dissociation of COMs by the external interstellar radiation field; and iii) the density...

  20. Unified Microscopic-Macroscopic Monte Carlo Simulations of Complex Organic Molecule Chemistry in Cold Cores

    Science.gov (United States)

    Chang, Qiang; Herbst, Eric

    2016-03-01

    The recent discovery of methyl formate and dimethyl ether in the gas phase of cold cores with temperatures as cold as 10 K challenges our previous astrochemical models concerning the formation of complex organic molecules (COMs). The strong correlation between the abundances and distributions of methyl formate and dimethyl ether further shows that current astrochemical models may be missing important chemical processes in cold astronomical sources. We investigate a scenario in which COMs and the methoxy radical can be formed on dust grains via a so-called chain reaction mechanism, in a similar manner to CO2. A unified gas-grain microscopic-macroscopic Monte Carlo approach with both normal and interstitial sites for icy grain mantles is used to perform the chemical simulations. Reactive desorption with varying degrees of efficiency is included to enhance the nonthermal desorption of species formed on cold dust grains. In addition, varying degrees of efficiency for the surface formation of methoxy are also included. The observed abundances of a variety of organic molecules in cold cores can be reproduced in our models. The strong correlation between the abundances of methyl formate and dimethyl ether in cold cores can also be explained. Nondiffusive chemical reactions on dust grain surfaces may play a key role in the formation of some COMs.

  1. Shell and core cross-linked poly(L-lysine)/poly(acrylic acid) complex micelles.

    Science.gov (United States)

    Hsieh, Yi-Hsuan; Hsiao, Yung-Tse; Jan, Jeng-Shiung

    2014-12-21

    We report the versatility of polyion complex (PIC) micelles for the preparation of shell and core cross-linked (SCL and CCL) micelles with their surface properties determined by the constituent polymer composition and cross-linking agent. The negatively and positively charged PIC micelles with their molecular structure and properties depending on the mixing weight percentage and polymer molecular weight were first prepared by mixing the negatively and positively charged polyions, poly(acrylic acid) (PAA) and poly(L-lysine) (PLL). The feasibility of preparing SCL micelles was demonstrated by cross-linking the shell of the negatively and positively charged micelles using cystamine and genipin, respectively. The core of the micelles can be cross-linked by silica deposition to stabilize the assemblies. The shell and/or core cross-linked micelles exhibited excellent colloid stability upon changing solution pH. The drug release from the drug-loaded SCL micelles revealed that the controllable permeability of the SCL micelles can be achieved by tuning the cross-linking degree and the SCL micelles exhibited noticeable pH-responsive behavior with accelerated release under acidic conditions. With the versatility of cross-linking strategies, it is possible to prepare a variety of SCL and CCL micelles from PIC micelles.

  2. Unfolding communities in large complex networks: Combining defensive and offensive label propagation for core extraction

    CERN Document Server

    Šubelj, Lovro; 10.1103/PhysRevE.83.036103

    2011-01-01

    Label propagation has proven to be a fast method for detecting communities in large complex networks. Recent developments have also improved the accuracy of the approach, however, a general algorithm is still an open issue. We present an advanced label propagation algorithm that combines two unique strategies of community formation, namely, defensive preservation and offensive expansion of communities. Two strategies are combined in a hierarchical manner, to recursively extract the core of the network, and to identify whisker communities. The algorithm was evaluated on two classes of benchmark networks with planted partition and on almost 25 real-world networks ranging from networks with tens of nodes to networks with several tens of millions of edges. It is shown to be comparable to the current state-of-the-art community detection algorithms and superior to all previous label propagation algorithms, with comparable time complexity. In particular, analysis on real-world networks has proven that the algorithm ...

  3. Unified Microscopic-Macroscopic Monte Carlo Simulations of Complex Organic Molecule Chemistry in Cold Cores

    CERN Document Server

    Chang, Qiang

    2016-01-01

    The recent discovery of methyl formate and dimethyl ether in the gas phase of cold cores with temperatures as cold as 10 K challenges our previous astrochemical models concerning the formation of complex organic molecules. The strong correlation between the abundances and distributions of methyl formate and dimethyl ether further shows that current astrochemical models may be missing important chemical processes in cold astronomical sources. We investigate a scenario in which complex organic molecules and the methoxy radical can be formed on dust grains via a so-called "chain reaction" mechanism, in a similar manner to CO$_2$. A unified gas-grain microscopic-macroscopic Monte Carlo approach with both normal and interstitial sites for icy grain mantles is used to perform the chemical simulations. Reactive desorption with varying degrees of efficiency is included to enhance the non-thermal desorption of species formed on cold dust grains. In addition, varying degrees of efficiency for the surface formation of m...

  4. Crystal structure of the RC-LH1 core complex from Rhodopseudomonas palustris.

    Science.gov (United States)

    Roszak, Aleksander W; Howard, Tina D; Southall, June; Gardiner, Alastair T; Law, Christopher J; Isaacs, Neil W; Cogdell, Richard J

    2003-12-12

    The crystal structure at 4.8 angstrom resolution of the reaction center-light harvesting 1 (RC-LH1) core complex from Rhodopseudomonas palustris shows the reaction center surrounded by an oval LH1 complex that consists of 15 pairs of transmembrane helical alpha- and beta-apoproteins and their coordinated bacteriochlorophylls. Complete closure of the RC by the LH1 is prevented by a single transmembrane helix, out of register with the array of inner LH1 alpha-apoproteins. This break, located next to the binding site in the reaction center for the secondary electron acceptor ubiquinone (UQB), may provide a portal through which UQB can transfer electrons to cytochrome b/c1.

  5. Geologic evolution of the Cordillera Darwin orogenic core complex, Southern Andes

    Science.gov (United States)

    Nelson, E. P.

    1981-08-01

    Located in the east-west trending Andes of Tierra del Fuego is a structural culmination exposing deeper crustal levels than in surrounding areas, termed an orogenic core complex because of the localization there of relatively high-grade metamorphism, intense polyphase deformation, and differential uplift. Strongly deformed and regionally metamorphosed pre-Late Jurassic basement rocks mainly of sedimentary origin are unconformably overlain by a cover sequence of Upper Jurassic silicic-intermediate volcanic rocks (Tobifera Formation) and Lower Cretaceous clastic sedimentary rocks (Yahgan Formation). The D1 and D2 phases produced major and minor fold structures, extension and intersection lineations, and axial planar and transposition foliations in complex patterns similar to those in other collision-type orogens. The Darwin and Beagle suites show affinities with S- and I-type granitic suites respectively.

  6. Low complexity MIMO method based on matrix transformation for few-mode multi-core optical transmission system

    Science.gov (United States)

    Pan, Xiaolong; Liu, Bo; Li, Li; Tian, Qinghua

    2016-07-01

    This paper proposes and demonstrates a low complexity multiple-input multiple-output (MIMO) equalization digital signal processing (DSP) method for the few mode multi-core (FMMC) fiber optical transmission system. The MIMO equalization algorithm offers adaptive equalization taps according to the degree of crosstalk in cores or modes, which eliminates the interference among different modes and cores in space division multiplexing (SDM) transmission system. Compared with traditional MIMO method, the proposed scheme has increased the convergence rate by 4 times and reduced the number of finite impulse response (FIR) filters by 55% when the numbers of mode and core are three.

  7. A high-resolution study of complex organic molecules in hot cores

    Science.gov (United States)

    Calcutt, Hannah; Viti, Serena; Codella, Claudio; Beltrán, Maria T.; Fontani, Francesco; Woods, Paul M.

    2014-10-01

    We present the results of a line identification analysis using data from the IRAM Plateau de Bure Plateau de Bure Interferometer, focusing on six massive star-forming hot cores: G31.41+0.31, G29.96-0.02, G19.61-0.23, G10.62-0.38, G24.78+0.08A1 and G24.78+0.08A2. We identify several transitions of vibrationally excited methyl formate (HCOOCH3) for the first time in these objects as well as transitions of other complex molecules, including ethyl cyanide (C2H5CN), and isocyanic acid (HNCO). We also postulate a detection of one transition of glycolaldehyde (CH2(OH)CHO) in two new hot cores. We find G29.96-0.02, G19.61-0.23, G24.78+0.08A1 and G24.78+0.08A2 to be chemically very similar. G31.41+0.31, however, is chemically different: it manifests a larger chemical inventory and has significantly larger column densities. We suggest that it may represent a different evolutionary stage to the other hot cores in the sample, or it may surround a star with a higher mass. We derive column densities for methyl formate in G31.41+0.31, using the rotation diagram method, of 4 × 1017 cm-2 and a Trot of ˜170 K. For G29.96-0.02, G24.78+0.08A1 and G24.78+0.08A2, glycolaldehyde, methyl formate and methyl cyanide, all seem to trace the same material and peak at roughly the same position towards the dust emission peak. For G31.41+0.31, however, glycolaldehyde shows a different distribution to methyl formate and methyl cyanide and seems to trace the densest, most compact inner part of hot cores.

  8. Interactions between plutonism and detachments during metamorphic core complex formation, Serifos Island (Cyclades, Greece)

    Science.gov (United States)

    Rabillard, Aurélien; Arbaret, Laurent; Jolivet, Laurent; Le Breton, Nicole; Gumiaux, Charles; Augier, Romain; Grasemann, Bernhard

    2015-06-01

    In order to better understand the interactions between plutonic activity and strain localization during metamorphic core complex formation, the Miocene granodioritic pluton of Serifos (Cyclades, Greece) is studied. This pluton (11.6-9.5 Ma) intruded the Cycladic Blueschists during thinning of the Aegean domain along a system of low-angle normal faults belonging to the south dipping West Cycladic Detachment System (WCDS). Based on structural fieldwork, together with microstructural observations and anisotropy of magnetic susceptibility, we recognize a continuum of deformation from magmatic to brittle conditions within the magmatic body. This succession of deformation events is kinematically compatible with the development of the WCDS. The architecture of the pluton shows a marked asymmetry resulting from its interaction with the detachments. We propose a tectonic scenario for the emplacement of Serifos pluton and its subsequent cooling during the Aegean extension: (1) A first stage corresponds to the metamorphic core complex initiation and associated southwestward shearing along the Meghàlo Livadhi detachment. (2) In the second stage, the Serifos pluton has intruded the dome at shallow crustal level, piercing through the ductile/brittle Meghàlo Livadhi detachment. Southwest directed extensional deformation was contemporaneously transferred upward in the crust along the more localized Kàvos Kiklopas detachment. (3) The third stage was marked by synmagmatic extensional deformation and strain localization at the contact between the pluton and the host rocks resulting in nucleation of narrow shear zones, which (4) continued to develop after the pluton solidification.

  9. Core-Shell Coating Silicon Anode Interfaces with Coordination Complex for Stable Lithium-Ion Batteries.

    Science.gov (United States)

    Zhou, Jinqiu; Qian, Tao; Wang, Mengfan; Xu, Na; Zhang, Qi; Li, Qun; Yan, Chenglin

    2016-03-02

    In situ core-shell coating was used to improve the electrochemical performance of Si-based anodes with polypyrrole-Fe coordination complex. The vast functional groups in the organometallic coordination complex easily formed hydrogen bonds when in situ modifying commercial Si nanoparticles. The incorporation of polypyrrole-Fe resulted in the conformal conductive coating surrounding each Si nanoparticle, not only providing good electrical connection to the particles but also promoting the formation of a stable solid-electrolyte-interface layer on the Si electrode surface, enhancing the cycling properties. As an anode material for Li-ion batteries, modified silicon powders exhibited high reversible capacity (3567 mAh/g at 0.3 A/g), good rate property (549.12 mAh/g at 12 A/g), and excellent cycling performance (reversible capacity of 1500 mAh/g after 800 cycles at 1.2 A/g). The constructed novel concept of core-shell coating Si particles presented a promising route for facile and large-scale production of Si-based anodes for extremely durable Li-ion batteries, which provided a wide range of applications in the field of energy storage of the renewable energy derived from the solar energy, hydropower, tidal energy, and geothermal heat.

  10. Protein film voltammetry and co-factor electron transfer dynamics in spinach photosystem II core complex.

    Science.gov (United States)

    Zhang, Yun; Magdaong, Nikki; Frank, Harry A; Rusling, James F

    2014-05-01

    Direct protein film voltammetry (PFV) was used to investigate the redox properties of the photosystem II (PSII) core complex from spinach. The complex was isolated using an improved protocol not used previously for PFV. The PSII core complex had high oxygen-evolving capacity and was incorporated into thin lipid and polyion films. Three well-defined reversible pairs of reduction and oxidation voltammetry peaks were observed at 4 °C in the dark. Results were similar in both types of films, indicating that the environment of the PSII-bound cofactors was not influenced by film type. Based on comparison with various control samples including Mn-depleted PSII, peaks were assigned to chlorophyll a (Chl a) (Em = -0.47 V, all vs. NHE, at pH 6), quinones (-0.12 V), and the manganese (Mn) cluster (Em = 0.18 V). PFV of purified iron heme protein cytochrome b-559 (Cyt b-559), a component of PSII, gave a partly reversible peak pair at 0.004 V that did not have a potential similar to any peaks observed from the intact PSII core complex. The closest peak in PSII to 0.004 V is the 0.18 V peak that was found to be associated with a two-electron process, and thus is inconsistent with iron heme protein voltammetry. The -0.47 V peak had a peak potential and peak potential-pH dependence similar to that found for purified Chl a incorporated into DMPC films. The midpoint potentials reported here may differ to various extents from previously reported redox titration data due to the influence of electrode double-layer effects. Heterogeneous electron transfer (hET) rate constants were estimated by theoretical fitting and digital simulations for the -0.47 and 0.18 V peaks. Data for the Chl a peaks were best fit to a one-electron model, while the peak assigned to the Mn cluster was best fit by a two-electron/one-proton model.

  11. Mesozoic burial, Mesozoic and Cenozoic exhumation of the Funeral Mountains core complex, Death Valley, Southeastern California

    Science.gov (United States)

    Beyene, Mengesha Assefa

    2011-12-01

    The Funeral Mountains of Death Valley National Park, CA, provide an opportunity to date metamorphism resulting from crustal shortening and subsequent episodic extensional events in the Sevier hinterland. It was not clear whether crustal shortening and thus peak temperature metamorphism in the hinterland of the Sevier-Laramide orogenic wedge have occurred whether in Late Jurassic, Early Cretaceous, Late Cretaceous or somewhere between. Particularly ambiguous is the timing of crustal shortening in the deep levels of the hinterland of the Sevier belt, now manifest in the metamorphic core complexes, and how and when these middle-to-lower crustal rocks were exhumed. A 6-point garnet and a whole rock Savillax isochron from middle greenschist facies pelitic schist of the southeastern Funeral Mountains core complex yields an age of 162.1 +/- 5.8 Ma (2sigma). Composite PT paths determined from growth-zoned garnets from the same samples show a nearly isothermal pressure increase of ˜2 kbar at ˜490°C, suggesting thrust burial at 162.1 +/- 5.8 Ma. A second sample of Johnnie Formation from the comparatively higher metamorphic grade area to the northwest (East of Chloride Cliff) yielded an age of 172.9 +/- 4.9 Ma (2sigma) suggesting an increase of thrust burial age towards the higher grade rocks (northwest part of the core complex), consistent with paleo-depth interpretation and metamorphic grade. 40Ar/ 39Ar muscovite ages along footwall of the Boundary Canyon detachment fault and intra-core Chloride Cliff shear zone exhibit significant 40Ar/39Ar muscovite age differences. For samples from the immediate footwall of BCD, the pattern of ages decreasing toward the northwest is consistent with differences in depth of metamorphism, and for Late Cretaceous, top-to-northwest exhumation by motion along the precursor BCD; consistent with mesoscopic and microscopic kinematic studies. Samples from the footwall of the structurally-lower Chloride Cliff shear zone yield Tertiary 40Ar/39Ar

  12. Complex inner core of the Earth: The last frontier of global seismology

    Science.gov (United States)

    Tkalčić, Hrvoje

    2015-03-01

    The days when the Earth's inner core (IC) was viewed as a homogeneous solid sphere surrounded by the liquid outer core (OC) are now behind us. Due to a limited number of data sampling the IC and a lack of experimentally controlled conditions in the deep Earth studies, it has been difficult to scrutinize competitive hypotheses in this active area of research. However, a number of new concepts linking IC structure and dynamics has been proposed lately to explain different types of seismological observations. A common denominator of recent observational work on the IC is increased complexity seen in IC physical properties such as its isotropic and anisotropic structure, attenuation, inner core boundary (ICB) topography, and its rotational dynamics. For example, small-scale features have been observed to exist as a widespread phenomenon in the uppermost inner core, probably superimposed on much longer-scale features. The characterization of small-scale features sheds light on the nature of the solidification process and helps in understanding seismologically observed hemispherical dichotomy of the IC. The existence of variations in the rate and level of solidification is a plausible physical outcome in an environment where vigorous compositional convection in the OC and variations in heat exchange across the ICB may control the process of crystal growth. However, further progress is hindered by the fact that the current traveltime data of PKIKP waves traversing the IC do not allow discriminating between variations in isotropic P wave velocity and velocity anisotropy. Future studies of attenuation in the IC might provide crucial information about IC structure, although another trade-off exists—that of the relative contribution of scattering versus viscoelastic attenuation and the connection with the material properties. Future installations of dense arrays, cross paths of waves that sample the IC, and corresponding array studies will be a powerful tool to image and

  13. Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA

    DEFF Research Database (Denmark)

    Andersen, Christian Brix Folsted; Ballut, Lionel; Johansen, Jesper Sanderhoff;

    2006-01-01

    In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric e...

  14. Complex molecules in the hot core of the low-mass protostar NGC 1333 IRAS 4A

    NARCIS (Netherlands)

    Bottinelli, S; Ceccarelli, C; Lefloch, B; Williams, JP; Castets, A; Caux, E; Cazaux, S; Maret, S; Parise, B; Tielens, AGGM

    2004-01-01

    We report the detection of complex molecules (HCOOCH3, HCOOH, and CH3CN), signposts of a hot core like region, toward the low-mass Class 0 source NGC 1333 IRAS 4A. This is the second low-mass protostar in which such complex molecules have been searched for and reported, the other source being IRAS

  15. Ductile strain rate recorded in the Symvolon syn-extensional plutonic body (Rhodope core complex, Greece)

    Science.gov (United States)

    Cirrincione, Rosolino; Fazio, Eugenio; Ortolano, Gaetano; Fiannacca, Patrizia; Kern, Hartmut; Mengel, Kurt; Pezzino, Antonino; Punturo, Rosalda

    2016-04-01

    The present contribution deals with quantitative microstructural analysis, which was performed on granodiorites of the syn-tectonic Symvolon pluton (Punturo et al., 2014) at the south-western boundary of the Rhodope Core Complex (Greece). Our purpose is the quantification of ductile strain rate achieved across the pluton, by considering its cooling gradient from the centre to the periphery, using the combination of a paleopiezometer (Shimizu, 2008) and a quartz flow law (Hirth et al., 2001). Obtained results, associated with a detailed cooling history (Dinter et al., 1995), allowed us to reconstruct the joined cooling and strain gradient evolution of the pluton from its emplacement during early Miocene (ca. 700°C at 22 Ma) to its following cooling stage (ca. 500-300°C at 15 Ma). Shearing temperature values were constrained by means of a thermodynamic approach based on the recognition of syn-shear assemblages at incremental strain; to this aim, statistical handling of mineral chemistry X-Ray maps was carried out on microdomains detected at the tails of porphyroclasts. Results indicate that the strain/cooling gradients evolve "arm in arm" across the pluton, as also testified by the progressive development of mylonitic fabric over the magmatic microstructures approaching the host rock. References • Dinter, D. A., Macfarlane, A., Hames, W., Isachsen, C., Bowring, S., and Royden, L. (1995). U-Pb and 40Ar/39Ar geochronology of the Symvolon granodiorite: Implications for the thermal and structural evolution of the Rhodope metamorphic core complex, northeastern Greece. Tectonics, 14 (4), 886-908. • Shimizu, I. (2008). Theories and applicability of grain size piezometers: The role of dynamic recrystallization mechanisms. Journal of Structural Geology, 30 (7), 899-917. • Hirth, G., Teyssier, C., and Dunlap, J. W. (2001). An evaluation of quartzite flow laws based on comparisons between experimentally and naturally deformed rocks. International Journal of Earth

  16. Maghemite (hematite) core (shell) nanorods via thermolysis of a molecular solid of Fe-complex.

    Science.gov (United States)

    Chaudhari, N S; Warule, S S; Muduli, S; Kale, B B; Jouen, S; Lefez, B; Hannoyer, B; Ogale, S B

    2011-08-21

    An Fe-metal complex with 2'-hydroxy chalcone (2'-HC) ligands [Fe(III) (2'-hydroxy chalcone)(3)] is synthesized by a chemical route and is subjected to different thermal treatments. Upon thermolysis in air at 450 °C for 3 h the complex yields maghemite (γ-Fe(2)O(3)) nanorods with a thin hematite (α-Fe(2)O(3)) shell. X-Ray diffraction (XRD), Mössbauer spectroscopy, diffuse reflectance spectroscopy (UV-DRS), high resolution transmission electron microscopy (HR-TEM), field emission scanning electron microscopy (FE-SEM) and vibrating sample magnetometry (VSM) are used to characterize the samples. The stability of the ligand and the Fe-complex is further examined by using thermogravimmetric/differential thermal analysis (TGA/DTA). We suggest a residual ligand controlled mechanism for the formation of an anisotropic nanostructure in a crumbling molecular solid undergoing ligand decomposition. Since the band gap of iron oxide is in the visible range, we explored the use of our core shell nano-rod sample for photocatalytic activity for H(2) generation by H(2)S splitting under solar light. We observed high photocatalytic activity for hydrogen generation (75 ml h(-1)). This journal is © The Royal Society of Chemistry 2011

  17. The gas phase origin of complex organic molecules precursors in prestellar cores

    Science.gov (United States)

    Bacmann, A.; Faure, A.

    2016-05-01

    Complex organic molecules (COMs) have long been observed in the warm regions surrounding nascent protostars. The recent discovery of oxygen-bearing COMs like methyl formate or dimethyl ether in prestellar cores (Bacmann et al. [2]), where gas and dust temperatures rarely exceed 10-15 K, has challenged the previously accepted models according to which COM formation relied on the diffusion of heavy radicals on warm (˜30 K) grains. Following these detections, new questions have arisen: do non-thermal processes play a role in increasing radical mobility or should new gas-phase routes be explored? The radicals involved in the formation of the aforementioned COMs, HCO and CH3O represent intermediate species in the grain-surface synthesis of methanol which proceeds via successive hydrogenations of CO molecules in the ice. We present here observations of methanol and its grain-surface precursors HCO, H2CO, CH3O in a sample of prestellar cores and derive their relative abundances. We find that the relative abundances HCO:H2CO:CH3O:CH3OH are constant across the core sample, close to 10:100:1:100. Our results also show that the amounts of HCO and CH3O are consistent with a gas-phase synthesis of these species from H2CO and CH3OH via radical-neutral or ion-molecule reactions followed by dissociative recombinations. Thus, while grain chemistry is necessary to explain the abundances of the parent volatile CH3OH, and possibly H2CO, the reactive species HCO and CH3O might be daughter molecules directly produced in the gas-phase.

  18. Complex Coacervate Core Micelles with Spectroscopic Labels for Diffusometric Probing of Biopolymer Networks.

    Science.gov (United States)

    Bourouina, Nadia; de Kort, Daan W; Hoeben, Freek J M; Janssen, Henk M; Van As, Henk; Hohlbein, Johannes; van Duynhoven, John P M; Kleijn, J Mieke

    2015-11-24

    We present the design, preparation, and characterization of two types of complex coacervate core micelles (C3Ms) with cross-linked cores and spectroscopic labels and demonstrate their use as diffusional probes to investigate the microstructure of percolating biopolymer networks. The first type consists of poly(allylamine hydrochloride) (PAH) and poly(ethylene oxide)-poly(methacrylic acid) (PEO-b-PMAA), labeled with ATTO 488 fluorescent dyes. We show that the size of these probes can be tuned by choosing the length of the PEO-PMAA chains. ATTO 488-labeled PEO113-PMAA15 micelles are very bright with 18 dye molecules incorporated into their cores. The second type is a (19)F-labeled micelle, for which we used PAH and a (19)F-labeled diblock copolymer tailor-made from poly(ethylene oxide)-poly(acrylic acid) (mPEO79-b-PAA14). These micelles contain approximately 4 wt % of (19)F and can be detected by (19)F NMR. The (19)F labels are placed at the end of a small spacer to allow for the necessary rotational mobility. We used these ATTO- and (19)F-labeled micelles to probe the microstructures of a transient gel (xanthan gum) and a cross-linked, heterogeneous gel (κ-carrageenan). For the transient gel, sensitive optical diffusometry methods, including fluorescence correlation spectroscopy, fluorescence recovery after photobleaching, and super-resolution single nanoparticle tracking, allowed us to measure the diffusion coefficient in networks with increasing density. From these measurements, we determined the diameters of the constituent xanthan fibers. In the heterogeneous κ-carrageenan gels, bimodal nanoparticle diffusion was observed, which is a signpost of microstructural heterogeneity of the network.

  19. The Spatial Distribution of Complex Organic Molecules in the L1544 Pre-stellar Core

    Science.gov (United States)

    Jiménez-Serra, Izaskun; Vasyunin, Anton I.; Caselli, Paola; Marcelino, Nuria; Billot, Nicolas; Viti, Serena; Testi, Leonardo; Vastel, Charlotte; Lefloch, Bertrand; Bachiller, Rafael

    2016-01-01

    The detection of complex organic molecules (COMs) toward cold sources such as pre-stellar cores (with T<10 K), has challenged our understanding of the formation processes of COMs in the interstellar medium. Recent modelling on COM chemistry at low temperatures has provided new insight into these processes predicting that COM formation depends strongly on parameters such as visual extinction and the level of CO freeze out. We report deep observations of COMs toward two positions in the L1544 pre-stellar core: the dense, highly-extinguished continuum peak with AV ≥30 mag within the inner 2700 au; and a low-density shell with average AV ~7.5-8 mag located at 4000 au from the core’s center and bright in CH3OH. Our observations show that CH3O, CH3OCH3 and CH3CHO are more abundant (by factors ~2-10) toward the low-density shell than toward the continuum peak. Other COMs such as CH3OCHO, c-C3H2O, HCCCHO, CH2CHCN and HCCNC show slight enhancements (by factors ≤3) but the associated uncertainties are large. This suggests that COMs are actively formed and already present in the low-density shells of pre-stellar cores. The modelling of the chemistry of O-bearing COMs in L1544 indicates that these species are enhanced in this shell because i) CO starts freezing out onto dust grains driving an active surface chemistry; ii) the visual extinction is sufficiently high to prevent the UV photo-dissociation of COMs by the external interstellar radiation field; and iii) the density is still moderate to prevent severe depletion of COMs onto grains. PMID:27733899

  20. Multi-core CPU or GPU-accelerated Multiscale Modeling for Biomolecular Complexes.

    Science.gov (United States)

    Liao, Tao; Zhang, Yongjie; Kekenes-Huskey, Peter M; Cheng, Yuhui; Michailova, Anushka; McCulloch, Andrew D; Holst, Michael; McCammon, J Andrew

    2013-07-01

    Multi-scale modeling plays an important role in understanding the structure and biological functionalities of large biomolecular complexes. In this paper, we present an efficient computational framework to construct multi-scale models from atomic resolution data in the Protein Data Bank (PDB), which is accelerated by multi-core CPU and programmable Graphics Processing Units (GPU). A multi-level summation of Gaus-sian kernel functions is employed to generate implicit models for biomolecules. The coefficients in the summation are designed as functions of the structure indices, which specify the structures at a certain level and enable a local resolution control on the biomolecular surface. A method called neighboring search is adopted to locate the grid points close to the expected biomolecular surface, and reduce the number of grids to be analyzed. For a specific grid point, a KD-tree or bounding volume hierarchy is applied to search for the atoms contributing to its density computation, and faraway atoms are ignored due to the decay of Gaussian kernel functions. In addition to density map construction, three modes are also employed and compared during mesh generation and quality improvement to generate high quality tetrahedral meshes: CPU sequential, multi-core CPU parallel and GPU parallel. We have applied our algorithm to several large proteins and obtained good results.

  1. Effect of surface conditioning modalities on the repair bond strength of resin composite to the zirconia core / veneering ceramic complex.

    Science.gov (United States)

    Ozcan, Mutlu; Valandro, Luiz Felipe; Pereira, Sarina Maciel; Amaral, Regina; Bottino, Marco Antonio; Pekkan, Gurel

    2013-06-01

    This study evaluated the effect of different surface conditioning protocols on the repair strength of resin composite to the zirconia core / veneering ceramic complex, simulating the clinical chipping phenomenon. Forty disk-shaped zirconia core (Lava Zirconia, 3M ESPE) (diameter: 3 mm) specimens were veneered circumferentially with a feldspathic veneering ceramic (VM7, Vita Zahnfabrik) (thickness: 2 mm) using a split metal mold. They were then embedded in autopolymerizing acrylic with the bonding surfaces exposed. Specimens were randomly assigned to one of the following surface conditioning protocols (n = 10 per group): group 1, veneer: 4% hydrofluoric acid (HF) (Porcelain Etch) + core: aluminum trioxide (50-µm Al2O3) + core + veneer: silane (ESPE-Sil); group 2: core: Al2O3 (50 µm) + veneer: HF + core + veneer: silane; group 3: veneer: HF + core: 30 µm aluminum trioxide particles coated with silica (30 µm SiO2) + core + veneer: silane; group 4: core: 30 µm SiO2 + veneer: HF + core + veneer: silane. Core and veneer ceramic were conditioned individually but no attempt was made to avoid cross contamination of conditioning, simulating the clinical intraoral repair situation. Adhesive resin (VisioBond) was applied to both the core and the veneer ceramic, and resin composite (Quadrant Posterior) was bonded onto both substrates using polyethylene molds and photopolymerized. After thermocycling (6000 cycles, 5°C-55°C), the specimens were subjected to shear bond testing using a universal testing machine (1 mm/min). Failure modes were identified using an optical microscope, and scanning electron microscope images were obtained. Bond strength data (MPa) were analyzed statistically using the non-parametric Kruskal-Wallis test followed by the Wilcoxon rank-sum test and the Bonferroni Holm correction (α = 0.05). Group 3 demonstrated significantly higher values (MPa) (8.6 ± 2.7) than those of the other groups (3.2 ± 3.1, 3.2 ± 3, and 3.1 ± 3.5 for groups 1, 2, and 4

  2. Constraints on the lithospheric structure of mid ocean ridges from oceanic core complex morphology

    Science.gov (United States)

    Larson, Mark Oscar

    The Mid-oceanic ridge system is a feature unique to Earth. It is one of the fundamental components of plate tectonics and reflects interior processes of mantle convection within the Earth. The thermal structure beneath the mid-ocean ridges has been the subject of several modeling studies. It is expected that the elastic thickness of the lithosphere is larger near the transform faults that bound mid-ocean ridge segments. Oceanic core complexes (OCCs), which are generally thought to result from long-lived fault slip and elastic flexure, have a shape that is sensitive to elastic thickness. By modeling the shape of OCCs emplaced along a ridge segment, it is possible to constraint elastic thickness and therefore the thermal structure of the plate and how it varies along-axis. This thesis builds upon previous studies that utilize thin plate flexure to reproduce the shape of OCCs. I compare OCC shape to a suite of models in which elastic thickness, fault dip, fault heave, crustal thickness, and axial infill are systematically varied. Using a grid search, I constrain the parameters that best reproduce the bathymetry and/or the slope of ten candidate OCCs identified along the 12°-15°N segment of the Mid-Atlantic Ridge. The lithospheric elastic thicknesses that explains these OCCs is thinner than previous investigators suggested and the fault planes dip more shallowly in the subsurface, although at an angle compatible with Anderson's theory of faulting. No relationships between model parameters and an oceanic core complexes location within a segment are identified with the exception that the OCCs located less than 20km from a transform fault have slightly larger elastic thickness than OCCs in the middle of the ridge segment.

  3. Episodic growth of a Late Cretaceous and Paleogene intrusive complex of pegmatitic leucogranite, Ruby Mountains core complex, Nevada, USA

    Science.gov (United States)

    Howard, K.A.; Wooden, J.L.; Barnes, C.G.; Premo, W.R.; Snoke, A.W.; Lee, S.-Y.

    2011-01-01

    Gneissic pegmatitic leucogranite forms a dominant component (>600 km3) of the midcrustal infrastructure of the Ruby Mountains-East Humboldt Range core complex (Nevada, USA), and was assembled and modified episodically into a batholithic volume by myriad small intrusions from ca. 92 to 29 Ma. This injection complex consists of deformed sheets and other bodies emplaced syntectonically into a stratigraphic framework of marble, calc-silicate rocks, quartzite, schist, and other granitoids. Bodies of pegmatitic granite coalesce around host-rock remnants, which preserve relict or ghost stratigraphy, thrusts, and fold nappes. Intrusion inflated but did not disrupt the host-rock structure. The pegmatitic granite increases proportionally downward from structurally high positions to the bottoms of 1-km-deep canyons where it constitutes 95%-100% of the rock. Zircon and monazite dated by U-Pb (sensitive high-resolution ion microprobe, SHRIMP) for this rock type cluster diffusely at ages near 92, 82(?), 69, 38, and 29 Ma, and indicate successive or rejuvenated igneous crystallization multiple times over long periods of the Late Cretaceous and the Paleogene. Initial partial melting of unexposed pelites may have generated granite forerunners, which were remobilized several times in partial melting events. Sources for the pegmatitic granite differed isotopically from sources of similar-aged interleaved equigranular granites. Dominant Late Cretaceous and fewer Paleogene ages recorded from some pegmatitic granite samples, and Paleogene-only ages from the two structurally deepest samples, together with varying zircon trace element contents, suggest several disparate ages of final emplacement or remobilization of various small bodies. Folded sills that merge with dikes that cut the same folds suggest that there may have been in situ partial remobilization. The pegmatitic granite intrusions represent prolonged and recurrent generation, assembly, and partial melting modification of a

  4. Enhancing oxidative stability in heated oils using core/shell structures of collagen and α-tocopherol complex.

    Science.gov (United States)

    Gim, Seo Yeong; Hong, Seungmi; Kim, Jisu; Kwon, YongJun; Kim, Mi-Ja; Kim, GeunHyung; Lee, JaeHwan

    2017-11-15

    In this study, collagen mesh structure was prepared by carrying α-tocopherol in the form of core/shell complex. Antioxidant properties of α-tocopherol loaded carriers were tested in moisture added bulk oils at 140°C. From one gram of collagen core/shell complex, 138mg α-tocopherol was released in medium chain triacylglycerol (MCT). α-Tocopherol was substantially protected against heat treatment when α-tocopherol was complexed in collagen core/shell. Oxidative stability in bulk oil was significantly enhanced by added collagen mesh structure or collagen core/shell complex with α-tocopherol compared to that in control bulk oils (pcore/shell with α-tocopherol (p>0.05). Results of DPPH loss in methanol demonstrated that collagen core/shell with α-tocopherol had significantly (pcore/shell complex is a promising way to enhance the stability of α-tocopherol and oxidative stability in oil-rich foods prepared at high temperature. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Origin of eclogite-bearing, domed, layered metamorphic complexes ("core complexes") in the D'entrecasteaux Islands, Papua New Guinea

    Science.gov (United States)

    Davies, Hugh L.; Warren, R. G.

    1988-02-01

    Compositionally layered metamorphic rocks of the D'Entrecasteaux Islands, Papua New Guinea, are folded into domes and antiforms bounded by faults parallel to metamorphic layering and foliation. The structures are broadly similar to the metamorphic "core complexes" of western North America. Lenses of ultramafic rock lie on the bounding faults, and the same faults have served as loci for Quaternary andesitic volcanic activity. Metamorphic grade in the northern islands (Goodenough and Fergusson) is amphibolite facies, with pockets of eclogite (Fergusson Island only) and granulite, and is greenschist facies in the southern island (Normanby). In all three islands there is a characteristic tectonostratigraphic sequence (FMU sequence) from felsic metamorphic rocks at base, or internally, through mafic metamorphic rocks to ultramafic rocks at top, or externally. The association of metamorphic and ultramafic rocks apparently developed in a north dipping Paleogene subduction system and was exhumed to upper crustal level in the Oligocene--Early Miocene, possibly by reversal of movement on faults in the former subduction system. Vigorous uplift and development of domes and antiforms in the Pliocene was triggered by westward propagation of the Woodlark Basin spreading ridge and was accompanied by rifting, rift-related magmatism, rapid erosion, and deposition of coarse sediment in the adjacent Trobriand Basin.

  6. Summary of micrographic analysis of fracture coating phases on drill cores from Pahute Mesa, Nevada Test Site. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-01

    The flow path between Pahute Mesa and the groundwater discharge area in Oasis Valley (approximately 18 miles to the southwest) is of concern due to the relatively short travel distance between a recharge area where underground nuclear testing has been conducted and the off-site water users. Groundwater flow and transport modeling by IT Corporation (IT) has shown rapid tritium transport in the volcanic rock aquifers along this flow path. The resultant estimates of rapid transport were based on water level data, limited hydraulic conductivity data, estimates of groundwater discharge rates in Oasis Valley, assumed porosities, and estimated retardation rates. Many of these parameters are poorly constrained and may vary considerably. Sampling and analytical techniques are being applied as an independent means to determine transport rates by providing an understanding of the geochemical processes that control solute movement along the flow path. As part of these geochemical investigations, this report summarizes the analysis of fracture coating mineral phases from drill core samples from the Pahute mesa area of the Nevada Test Site (NTS). Archived samples were collected based on the presence of natural fractures and on the types and abundance of secondary mineral phases present on those fracture surfaces. Mineral phases present along fracture surfaces are significant because, through the process of water-rock interaction, they can either contribute (as a result of dissolution) or remove (as a result of precipitation or adsorption) constituents from solution. Particular attention was paid to secondary calcite occurrences because they represent a potential source of exchangeable carbon and can interact with groundwater resulting in a modified isotopic signature and apparent water age.

  7. Three-Dimensional Architecture of the Human BRCA1-A Histone Deubiquitinase Core Complex

    Directory of Open Access Journals (Sweden)

    Otto J.P. Kyrieleis

    2016-12-01

    Full Text Available BRCA1 is a tumor suppressor found to be mutated in hereditary breast and ovarian cancer and plays key roles in the maintenance of genomic stability by homologous recombination repair. It is recruited to damaged chromatin as a component of the BRCA1-A deubiquitinase, which cleaves K63-linked ubiquitin chains attached to histone H2A and H2AX. BRCA1-A contributes to checkpoint regulation, repair pathway choice, and HR repair efficiency through molecular mechanisms that remain largely obscure. The structure of an active core complex comprising two Abraxas/BRCC36/BRCC45/MERIT40 tetramers determined by negative-stain electron microscopy (EM reveals a distorted V-shape architecture in which a dimer of Abraxas/BRCC36 heterodimers sits at the base, with BRCC45/Merit40 pairs occupying each arm. The location and ubiquitin-binding activity of BRCC45 suggest that it may provide accessory interactions with nucleosome-linked ubiquitin chains that contribute to their efficient processing. Our data also suggest how ataxia telangiectasia mutated (ATM-dependent BRCA1 dimerization may stabilize self-association of the entire BRCA1-A complex.

  8. Strain and flow in the metamorphic core complex of Ios Island (Cyclades, Greece)

    Science.gov (United States)

    Mizera, Marcel; Behrmann, Jan H.

    2016-10-01

    We have analysed strain and flow kinematics in the footwall of the South Cyclades Shear Zone (SCSZ), an important tectonic boundary within the Attic-Cycladic Crystalline Complex exposed on Ios Island, Cyclades, Aegean Sea. Coarse-grained augen gneisses in the basement unit flooring the SCSZ and forming a metamorphic core complex are excellently suited to measure finite strain using the Fry method and estimate the vorticity number ( W k) of flow with the "blocked-object" method. The results show that Oligo-Miocene exhumation of the basement unit during extension brought approximately 70 % N-S crustal stretching and up to 40 % subvertical shortening in a plane strain environment ( k = 0.99). Linear down-section strain decrease constrains a zone of contact deformation of the SCSZ of about 1.5 km thick. Kinematic vorticity number estimates suggest little deviation from pure shear ( W k = 0.26). Finite strain and W k are not correlated, indicating that the Ios basement and the overlying cover units were stretched compatibly. While the SCSZ is a localized zone of high strain, net displacement, however, may be restricted to about ten kilometres. This has important repercussions on large-scale tectonic models for extension in the Aegean.

  9. On the stability and morphology of complex coacervate core micelles: from spherical to wormlike micelles.

    Science.gov (United States)

    van der Kooij, Hanne M; Spruijt, Evan; Voets, Ilja K; Fokkink, Remco; Cohen Stuart, Martien A; van der Gucht, Jasper

    2012-10-01

    We present a systematic study of the stability and morphology of complex coacervate core micelles (C3Ms) formed from poly(acrylic acid) (PAA) and poly(N-methyl-2-vinylpyridinium)-b-poly(ethylene oxide) (PM2VP-b-PEO). We use polarized and depolarized dynamic and static light scattering, combined with small-angle X-ray scattering, to investigate how the polymer chain length and salt concentration affect the stability, size, and shape of these micelles. We show that C3Ms are formed in aqueous solution below a critical salt concentration, which increases considerably with increasing PAA and PM2VP length and levels off for long chains. This trend is in good agreement with a mean-field model of polyelectrolyte complexation based on the Voorn-Overbeek theory. In addition, we find that salt induces morphological changes in C3Ms when the PAA homopolymer is sufficiently short: from spherical micelles with a diameter of several tens of nanometers at low salt concentration to wormlike micelles with a contour length of several hundreds of nanometers just before the critical salt concentration. By contrast, C3Ms of long PAA homopolymers remain spherical upon addition of salt and shrink slightly. A critical review of existing literature on other C3Ms reveals that the transition from spherical to wormlike micelles is probably a general phenomenon, which can be rationalized in terms of a classical packing parameter for amphiphiles.

  10. A study of the core of the Shapley Concentration V. The A3528 complex

    CERN Document Server

    Bardelli, S; Baldi, A

    2000-01-01

    We present the results of a redshift survey of galaxies in the A3528 complex, a chain of interacting clusters in the core of the Shapley Concentration. This complex is characterized in the X-ray band by two pairs of roughly similar interacting clumps: one pair has been resolved as two optical Abell clusters (A3530 and A3532), while the two components of the other pair are both associated to A3528. The optical data show that the distance between the centers of A3530 and A3532 is smaller than their Abell radii, an indication of the existence at least of tidal interactions, and that the contours of galaxies in A3528 appear to be elongated in the North-South direction, pointing towards the A3530-A3532 pair. From our survey we obtained ~600 new radial velocity determinations: using this sample, we studied the dynamics of the four Abell clusters in this region (A3528, A3530, A3532 and A3535) and derived their mean velocities and velocity dispersions. Moreover we performed a substructure analysis, both bi-dimensiona...

  11. A taxonomic revision of the Phrynosoma douglasii species complex (Squamata: Phrynosomatidae).

    Science.gov (United States)

    Montanucci, Richard R

    2015-09-11

    Short-horned lizards (Phrynosoma douglasii species complex) occur throughout the inter-montane West and Great Plains of western North America. The comparative morphology and color pattern variation of short-horned lizards was studied in 3,174 specimens. Multivariate analyses of 20 morphological and color-pattern characters were applied to 977 specimens, and univariate statistics were summarized for 52 samples totaling 1,134 specimens. The results of the morphological data analyses support the recognition of P. douglasii (Bell 1828) as a distinct species, and the resurrection of P. brevirostris Girard 1858a and P. ornatissimum Girard 1858a as species distinct from P. hernandesi Girard 1858a. Two new species allied to P. brevirostris are described: P. bauri sp. nov. from the eastern plains of Colorado and northeastern New Mexico, southeastern Wyoming and southwestern Nebraska south of the North Platte River, and P. diminutum sp. nov. endemic to the San Luis Valley of southern Colorado and northern New Mexico. The Mexican taxon brachycercum Smith 1942 is reassigned as a subspecies of P. ornatissimum, based on non-discrete character differences and overall morphometric similarity. The ranges of P. hernandesi and P. ornatissimum broadly overlap in central New Mexico, the former taxon occupying the coniferous forests of disjunct mountain ranges, the latter occuring in the surrounding desert grasslands. Principal components analysis has revealed morphological evidence of hybridization where the two taxa meet, generally within ecotones between montane forest associations and grasslands. Principal components analysis has also revealed a high level of morphological variability in populations occurring in the Colorado Plateau region of northeastern Arizona, northwestern New Mexico, extreme southwestern Colorado and adjacent Utah. The evidence suggests that these populations arose through past hybridization between the two species. The taxon ornatum Girard 1858b, although

  12. Reinvestigation of aminoacyl-tRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex.

    Directory of Open Access Journals (Sweden)

    Kyutae Kim

    Full Text Available Twenty different aminoacyl-tRNA synthetases (ARSs link each amino acid to their cognate tRNAs. Individual ARSs are also associated with various non-canonical activities involved in neuronal diseases, cancer and autoimmune diseases. Among them, eight ARSs (D, EP, I, K, L, M, Q and RARS, together with three ARS-interacting multifunctional proteins (AIMPs, are currently known to assemble the multi-synthetase complex (MSC. However, the cellular function and global topology of MSC remain unclear. In order to understand the complex interaction within MSC, we conducted affinity purification-mass spectrometry (AP-MS using each of AIMP1, AIMP2 and KARS as a bait protein. Mass spectrometric data were funneled into SAINT software to distinguish true interactions from background contaminants. A total of 40, 134, 101 proteins in each bait scored over 0.9 of SAINT probability in HEK 293T cells. Complex-forming ARSs, such as DARS, EPRS, IARS, Kars, LARS, MARS, QARS and RARS, were constantly found to interact with each bait. Variants such as, AIMP2-DX2 and AIMP1 isoform 2 were found with specific peptides in KARS precipitates. Relative enrichment analysis of the mass spectrometric data demonstrated that TARSL2 (threonyl-tRNA synthetase like-2 was highly enriched with the ARS-core complex. The interaction was further confirmed by coimmunoprecipitation of TARSL2 with other ARS core-complex components. We suggest TARSL2 as a new component of ARS core-complex.

  13. Exceptional expansion and conservation of a CT-repeat complex in the core promoter of PAXBP1 in primates.

    Science.gov (United States)

    Mohammadparast, Saeid; Bayat, Hadi; Biglarian, Akbar; Ohadi, Mina

    2014-08-01

    Adaptive evolution may be linked with the genomic distribution and function of short tandem repeats (STRs). Proximity of the core promoter STRs to the +1 transcription start site (TSS), and their mutable nature are characteristics that highlight those STRs as a novel source of interspecies variation. The PAXBP1 gene (alternatively known as GCFC1) core promoter contains the longest STR identified in a Homo sapiens gene core promoter. Indeed, this core promoter is a stretch of four consecutive CT-STRs. In the current study, we used the Ensembl, NCBI, and UCSC databases to analyze the evolutionary trend and functional implication of this CT-STR complex in six major lineages across vertebrates, including primates, non-primate mammals, birds, reptiles, amphibians, and fish. We observed exceptional expansion (≥4-repeats) and conservation of this CT-STR complex across primates, except prosimians, Microcebus murinus and Otolemur garnettii (Fisher exact Pprimate lineages. Different length alleles across the PAXBP1 core promoter CT-STRs significantly altered gene expression in vitro (Pprimates and non-primates. To our knowledge, this is the first instance of expansion and conservation of a STR complex co-occurring specifically with the primate lineage.

  14. A census of dense cores in the Aquila cloud complex: SPIRE/PACS observations from the Herschel Gould Belt survey

    CERN Document Server

    Konyves, V; Men'shchikov, A; Palmeirim, P; Arzoumanian, D; Schneider, N; Roy, A; Didelon, P; Maury, A; Shimajiri, Y; Di Francesco, J; Bontemps, S; Peretto, N; Benedettini, M; Bernard, J -Ph; Elia, D; Griffin, M J; Hill, T; Kirk, J; Ladjelate, B; Marsh, K; Martin, P G; Motte, F; Luong, Q Nguyen; Pezzuto, S; Roussel, H; Rygl, K L J; Sadavoy, S I; Schisano, E; Spinoglio, L; Ward-Thompson, D; White, G J

    2015-01-01

    We present and discuss the results of the Herschel Gould Belt survey observations in a ~11 deg^2 area of the Aquila molecular cloud complex at d~260 pc, imaged with the SPIRE/PACS cameras from 70 to 500 micron. We identify a complete sample of starless dense cores and embedded protostars in this region, and analyze their global properties and spatial distributions. We find a total of 651 starless cores, ~60% of which are gravitationally bound prestellar cores, and they will likely form stars in the future. We also detect 58 protostellar cores. The core mass function (CMF) derived for the prestellar cores is very similar in shape to the stellar initial mass function (IMF), supporting the earlier view that there is a close physical link between the IMF and the CMF. The global shift in mass scale observed between the CMF and the IMF is consistent with a typical star formation efficiency of ~40%. By comparing the numbers of starless cores to the number of young stellar objects, we estimate that the lifetime of pr...

  15. X-ray observations of complex temperature structure in the cool-core cluster A85

    Energy Technology Data Exchange (ETDEWEB)

    Schenck, David E.; Datta, Abhirup; Burns, Jack O. [Center for Astrophysics and Space Astronomy, Department of Astrophysical and Planetary Science, University of Colorado, Boulder, CO 80309 (United States); Skillman, Sam [Kavli Fellow, Kavli Institute for Particle Astrophysics and Cosmology, SLAC, CA 94025 (United States)

    2014-07-01

    X-ray observations were used to examine the complex temperature structure of A85, a cool-core galaxy cluster. Temperature features can provide evidence of merging events which shock heat the intracluster gas. Temperature maps were made from both Chandra and XMM-Newton observations. The combination of a new, long-exposure XMM observation and an improved temperature map binning technique produced the highest fidelity temperature maps of A85 to date. Hot regions were detected near the subclusters to the south and southwest in both the Chandra and XMM temperature maps. The presence of these structures implies A85 is not relaxed. The hot regions may indicate the presence of shocks. The Mach numbers were estimated to be ∼1.9 at the locations of the hot spots. Observational effects will tend to systematically reduce temperature jumps, so the measured Mach numbers are likely underestimated. Neither temperature map showed evidence for a shock in the vicinity of the presumed radio relic near the southwest subcluster. However, the presence of a weak shock cannot be ruled out. There was tension between the temperatures measured by the two instruments.

  16. X-ray Observations of Complex Temperature Structure in the Cool-core cluster Abell 85

    CERN Document Server

    Schenck, David; Burns, Jack; Skillman, Sam

    2014-01-01

    X-ray observations were used to examine the complex temperature structure of Abell 85, a cool-core galaxy cluster. Temperature features can provide evidence of merging events which shock heat the intracluster gas. Temperature maps were made from both \\textit{Chandra} and \\textit{XMM-Newton} obervations. The combination of a new, long-exposure \\textit{XMM} observation and an improved temperature map binning technique produced the highest fidelity temperature maps of A85 to date. Hot regions were detected near the subclusters to the South and Southwest in both the \\textit{Chandra} and \\textit{XMM} temperature maps. The presence of these structures implies A85 is not relaxed. The hot regions may indicate the presence of shocks. The Mach numbers were estimated to be $\\sim$1.9 at the locations of the hot spots. Observational effects will tend to systematically reduce temperature jumps, so the measured Mach numbers are likely underestimated. Neither temperature map showed evidence for a shock in the vicinity of the...

  17. The Fanconi anemia core complex is dispensable during somatic hypermutation and class switch recombination.

    Directory of Open Access Journals (Sweden)

    Peter H L Krijger

    Full Text Available To generate high affinity antibodies during an immune response, B cells undergo somatic hypermutation (SHM of their immunoglobulin genes. Error-prone translesion synthesis (TLS DNA polymerases have been reported to be responsible for all mutations at template A/T and at least a fraction of G/C transversions. In contrast to A/T mutations which depend on PCNA ubiquitination, it remains unclear how G/C transversions are regulated during SHM. Several lines of evidence indicate a mechanistic link between the Fanconi Anemia (FA pathway and TLS. To investigate the contribution of the FA pathway in SHM we analyzed FancG-deficient B cells. B cells deficient for FancG, an essential member of the FA core complex, were hypersensitive to treatment with cross-linking agents. However, the frequencies and nucleotide exchange spectra of SHM remained comparable between wild-type and FancG-deficient B cells. These data indicate that the FA pathway is not involved in regulating the outcome of SHM in mammals. In addition, the FA pathway appears dispensable for class switch recombination.

  18. Mazatan metamorphic core complex (Sonora, Mexico): structures along the detachment fault and its exhumation evolution

    Science.gov (United States)

    Granillo, Ricardo Vega; Calmus, Thierry

    2003-08-01

    The Mazatán Sierra is the southernmost metamorphic core complex (MCC) of the Tertiary extensional belt of the western Cordillera. Its structural and lithological features are similar to those found in other MCC in Sonora and Arizona. The lower plate is composed of Proterozoic igneous and metamorphic rocks intruded by Tertiary plutons, both of which are overprinted by mylonitic foliation and N70°E-trending stretching lineation. Ductile and brittle-ductile deformations were produced by Tertiary extension along a normal shear zone or detachment fault. Shear sense is consistent across the Sierra and indicates a top to the WSW motion. The lithology and fabric reflect variations in temperature and pressure conditions during extensional deformation. The upper plate consists mainly of Cambrian-Mississippian limestone and minor quartzite, covered by upper Cretaceous volcanic rocks, and then by Tertiary syntectonic sedimentary deposits with interbedded volcanic flows. Doming caused uplift and denudation of the detachment, as well as successive low-angle and high-angle normal faulting across the western slope of Mazatán Sierra. An 18±3 Ma apatite fission-track age was obtained for a sample of Proterozoic monzogranite from the lower plate. The mean fission-track length indicates rapid cooling and consequent rapid uplift of this sample during the last stage of crustal extension.

  19. Intrusive dike complexes, cumulate cores, and the extrusive growth of Hawaiian volcanoes

    Science.gov (United States)

    Flinders, Ashton F.; Ito, Garrett; Garcia, Michael O.; Sinton, John M.; Kauahikaua, Jim; Taylor, Brian

    2013-01-01

    The Hawaiian Islands are the most geologically studied hot-spot islands in the world yet surprisingly, the only large-scale compilation of marine and land gravity data is more than 45 years old. Early surveys served as reconnaissance studies only, and detailed analyses of the crustal-density structure have been limited. Here we present a new chain-wide gravity compilation that incorporates historical island surveys, recently published work on the islands of Hawai‘i, Kaua‘i, and Ni‘ihau, and >122,000 km of newly compiled marine gravity data. Positive residual gravity anomalies reflect dense intrusive bodies, allowing us to locate current and former volcanic centers, major rift zones, and a previously suggested volcano on Ka‘ena Ridge. By inverting the residual gravity data, we generate a 3-D view of the dense, intrusive complexes and olivine-rich cumulate cores within individual volcanoes and rift zones. We find that the Hāna and Ka‘ena ridges are underlain by particularly high-density intrusive material (>2.85 g/cm3) not observed beneath other Hawaiian rift zones. Contrary to previous estimates, volcanoes along the chain are shown to be composed of a small proportion of intrusive material (<30% by volume), implying that the islands are predominately built extrusively.

  20. Reduction of protein adsorption on silica and polystyrene surfaces due to coating with Complex Coacervate Core Micelles

    NARCIS (Netherlands)

    Brzozowska, A. M.; Hofs, B.; de Keizer, A.; Fokkink, R.; Stuart, Martien A. Cohen; Norde, W.

    2009-01-01

    The reduction of protein adsorption by a polymer brush formed upon adsorption of Complex Coacervate Core Micelles (C3Ms), consisting of a charged copolymer containing a neutral block and an oppositely charged homopolymer, on silica and polystyrene surfaces has been studied in situ using fixed angle

  1. Paleomagnetic evidence for an inverse rotation history of Western Anatolia during the exhumation of Menderes core complex

    NARCIS (Netherlands)

    Uzel, Bora; Langereis, Cornelis G.|info:eu-repo/dai/nl/073584223; Kaymakçı, Nuretdin; Sözbilir, Hasan; Özkaymak, Çağlar; Özkaptan, Murat

    2015-01-01

    Within the Aegean extensional system, the Izmir-Balikesir Transfer Zone (IBTZ) is a crucial element in the late Cenozoic evolution of western Anatolia since it accommodates the differential deformation between the Cycladic and the Menderes metamorphic core complexes. Here, we determine the rotationa

  2. A core MRB1 complex component is indispensable for RNA editing in insect and human infective stages of Trypanosoma brucei.

    Directory of Open Access Journals (Sweden)

    Michelle L Ammerman

    Full Text Available Uridine insertion/deletion RNA editing is a unique and vital process in kinetoplastids, required for creation of translatable open reading frames in most mitochondrially-encoded RNAs. Emerging as a key player in this process is the mitochondrial RNA binding 1 (MRB1 complex. MRB1 comprises an RNA-independent core complex of at least six proteins, including the GAP1/2 guide RNA (gRNA binding proteins. The core interacts in an RNA-enhanced or -dependent manner with imprecisely defined TbRGG2 subcomplexes, Armadillo protein MRB10130, and additional factors that comprise the dynamic MRB1 complex. Towards understanding MRB1 complex function in RNA editing, we present here functional characterization of the pentein domain-containing MRB1 core protein, MRB11870. Inducible RNAi studies demonstrate that MRB11870 is essential for proliferation of both insect vector and human infective stage T. brucei. MRB11870 ablation causes a massive defect in RNA editing, affecting both pan-edited and minimally edited mRNAs, but does not substantially affect mitochondrial RNA stability or processing of precursor transcripts. The editing defect in MRB1-depleted cells occurs at the initiation stage of editing, as pre-edited mRNAs accumulate. However, the gRNAs that direct editing remain abundant in the knockdown cells. To examine the contribution of MRB11870 to MRB1 macromolecular interactions, we tagged core complexes and analyzed their composition and associated proteins in the presence and absence of MRB11870. These studies demonstrated that MRB11870 is essential for association of GAP1/2 with the core, as well as for interaction of the core with other proteins and subcomplexes. Together, these data support a model in which the MRB1 core mediates functional interaction of gRNAs with the editing machinery, having GAP1/2 as its gRNA binding constituents. MRB11870 is a critical component of the core, essential for its structure and function.

  3. Analytic model for the complex effective index dispersion of metamaterial-cladding large-area hollow core fibers.

    Science.gov (United States)

    Zeisberger, Matthias; Tuniz, Alessandro; Schmidt, Markus A

    2016-09-05

    We present a mathematical model that allows interpreting the dispersion and attenuation of modes in hollow-core fibers (HCFs) on the basis of single interface reflection, giving rise to analytic and semi-analytic expressions for the complex effective indices in the case where the core diameter is large and the guiding is based on the reflection by a thin layer. Our model includes two core-size independent reflection parameters and shows the universal inverse-cubed core diameter dependence of the modal attenuation of HCFs. It substantially reduces simulation complexity and enables large scale parameter sweeps, which we demonstrate on the example of a HCF with a highly anisotropic metallic nanowire cladding, resembling an indefinite metamaterial at high metal filling fractions. We reveal design rules that allow engineering modal discrimination and show that metamaterial HCFs can principally have low losses at mid-IR wavelengths (model can be applied to a great variety of HCFs with large core diameters and can be used for advanced HCF design and performance optimization, in particular with regard to dispersion engineering and modal discrimination.

  4. Effect of pH on complex coacervate core micelles from Fe(III)-based coordination polymer.

    Science.gov (United States)

    Wang, Junyou; de Keizer, Arie; van Leeuwen, Herman P; Yan, Yun; Vergeldt, Frank; van As, Henk; Bomans, Paul H H; Sommerdijk, Nico A J M; Cohen Stuart, Martien A; van der Gucht, Jasper

    2011-12-20

    The effect of pH on iron-containing complex coacervate core micelles [Fe(III)-C3Ms] is investigated in this paper. The Fe(III)-C3Ms are formed by mixing cationic poly(N-methyl-2-vinylpyridinium iodide)-b-poly(ethylene oxide) [P2MVP(41)-b-PEO(205)] and anionic iron coordination polymers [Fe(III)-L(2)EO(4)] at stoichiometric charge ratio. Light scattering and Cryo-TEM have been performed to study the variations of hydrodynamic radius and core structure with changing pH. The hydrodynamic radius of Fe(III)-C3Ms is determined mainly by the corona and does not change very much in a broad pH range. However, Cryo-TEM pictures and magnetic relaxation measurements indicate that the structure of the micellar cores changes upon changing the pH, with a more crystalline, elongated shape and lower relaxivity at high pH. We attribute this to the formation of mixed iron complexes in the core, involving both the bis-ligand and hydroxide ions. These complexes are stabilized toward precipitation by the diblock copolymer.

  5. Mantle rock exposures at oceanic core complexes along mid-ocean ridges

    Directory of Open Access Journals (Sweden)

    Ciazela Jakub

    2015-12-01

    Full Text Available The mantle is the most voluminous part of the Earth. However, mantle petrologists usually have to rely on indirect geophysical methods or on material found ex situ. In this review paper, we point out the in-situ existence of oceanic core complexes (OCCs, which provide large exposures of mantle and lower crustal rocks on the seafloor on detachment fault footwalls at slow-spreading ridges. OCCs are a common structure in oceanic crust architecture of slow-spreading ridges. At least 172 OCCs have been identified so far and we can expect to discover hundreds of new OCCs as more detailed mapping takes place. Thirty-two of the thirty-nine OCCs that have been sampled to date contain peridotites. Moreover, peridotites dominate in the plutonic footwall of 77% of OCCs. Massive OCC peridotites come from the very top of the melting column beneath ocean ridges. They are typically spinel harzburgites and show 11.3–18.3% partial melting, generally representing a maximum degree of melting along a segment. Another key feature is the lower frequency of plagioclase-bearing peridotites in the mantle rocks and the lower abundance of plagioclase in the plagioclase-bearing peridotites in comparison to transform peridotites. The presence of plagioclase is usually linked to impregnation with late-stage melt. Based on the above, OCC peridotites away from segment ends and transforms can be treated as a new class of abyssal peridotites that differ from transform peridotites by a higher degree of partial melting and lower interaction with subsequent transient melt.

  6. Opposing shear senses in a subdetachment mylonite zone: Implications for core complex mechanics

    Science.gov (United States)

    Cooper, Frances J.; Platt, John P.; Platzman, Ellen S.; Grove, Marty J.; Seward, Gareth

    2010-08-01

    Global studies of metamorphic core complexes and low-angle detachment faults have highlighted a fundamental problem: Since detachments excise crustal section, the relationship between the mylonitic rocks in their footwalls and the brittle deformation in their hanging walls is commonly unclear. Mylonites could either reflect ductile deformation related to exhumation along the detachment fault, or they could be a more general feature of the extending middle crust that has been "captured" by the detachment. In the first case we would expect the kinematics of the mylonite zone to mirror the sense of movement on the detachment; in the second case both the direction and sense of shear in the mylonites could be different. The northern Snake Range décollement (NSRD) is a classic Basin and Range detachment fault with a well-documented top-east of displacement. We present structural, paleomagnetic, geochronological, and geothermometric evidence to suggest that the mylonite zone below the NSRD locally experienced phases of both east- and west-directed shear, inconsistent with movement along a single detachment fault. We therefore propose that the footwall mylonites represent a predetachment discontinuity in the middle crust that separated localized deformation above from distributed crustal flow below (localized-distributed transition (LDT)). The mylonites were subsequently captured by a moderately dipping brittle detachment that soled down to the middle crust and exhumed them around a rolling hinge into a subhorizontal orientation at the surface, producing the present-day NSRD. In this interpretation the brittle hanging wall represents a series of rotated upper crustal normal faults, whereas the mylonitic footwall represents one or more exhumed middle crustal discontinuities (LDTs).

  7. Exploring molecular complexity with ALMA (EMoCA): Detection of three new hot cores in Sagittarius B2(N)

    OpenAIRE

    Bonfand, M.; Belloche, A.; Menten, K. M.; Garrod, R. T.; Mueller, H. S. P.

    2017-01-01

    The SgrB2 molecular cloud contains several sites forming high-mass stars. SgrB2(N) is one of its main centers of activity. It hosts several compact and UCHII regions, as well as two known hot molecular cores (SgrB2(N1) and SgrB2(N2)), where complex organic molecules are detected. Our goal is to use the high sensitivity of ALMA to characterize the hot core population in SgrB2(N) and shed a new light on the star formation process. We use a complete 3 mm spectral line survey conducted with ALMA ...

  8. $k$-core percolation on complex networks: Comparing random, localized and targeted attacks

    CERN Document Server

    Yuan, Xin; Stanley, H Eugene; Havlin, Shlomo

    2016-01-01

    The type of malicious attack inflicting on networks greatly influences their stability under ordinary percolation in which a node fails when it becomes disconnected from the giant component. Here we study its generalization, $k$-core percolation, in which a node fails when it loses connection to a threshold $k$ number of neighbors. We study and compare analytically and by numerical simulations of $k$-core percolation the stability of networks under random attacks (RA), localized attacks (LA) and targeted attacks (TA), respectively. By mapping a network under LA or TA into an equivalent network under RA, we find that in both single and interdependent networks, TA exerts the greatest damage to the core structure of a network. We also find that for Erd\\H{o}s-R\\'{e}nyi (ER) networks, LA and RA exert equal damage to the core structure whereas for scale-free (SF) networks, LA exerts much more damage than RA does to the core structure.

  9. Multifrequency studies of massive cores with complex spatial and kinematic structures

    Science.gov (United States)

    Pirogov, L. E.; Shul'ga, V. M.; Zinchenko, I. I.; Zemlyanukha, P. M.; Patoka, A. N.; Tomasson, M.

    2016-10-01

    Five regions of massive-star formation have been observed in various molecular lines in the frequency range˜85-89 GHz. The studied regions comprise dense cores, which host young stellar objects. The physical parameters of the cores are estimated, including the kinetic temperatures (˜20-40 K), the sizes of the emitting regions (˜0.1-0.6 pc), and the virial masses (˜40-500 M ⊙). The column densities and abundances of various molecules are calculated assuming Local Thermodynamical Equilibrium(LTE). The core in 99.982+4.17, which is associated with the weakest IRAS source, is characterized by reduced molecular abundances. The molecular line widths decrease with increasing distance from the core centers ( b). For b ≳ 0.1 pc, the dependences Δ V ( b) are close to power laws (∝ b - p ), where p varies from ~0.2 to ~0.5, depending on the object. In four cores, the asymmetries of the optically thick HCN(1-0) and HCO+(1-0) lines indicates systematicmotions along the line of sight: collapse in two cores and expansion in two others. Approximate estimates of the accretion rates in the collapsing cores indicate that the forming stars have masses exceeding the solar mass.

  10. Tectono-geochemistry analyses of fault rocks in shear zone of metamorphic core complex in north Jiangxi, China

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Through a systematic sampling test and mass equilibrium analysis of the three sorts of complex assemblages (intrusive complex, tectonic complex and metamorphic complex) penetrating the metamorphic core complex (MCC) in the Xingzi area of north Jiangxi, the authors find that, like major elements, the trace elements of small ion radius, big specific gravity and high potential form the accumulative series in fault rocks, instead of divergence series. In rare earth elements, ΣREE and HREE are relatively centralized, characteristic of rising and Eu loss in the distribution pattern. Only on the upside of the ductile fault, there exist some phenomena contrary to the general rules, most of which are restricted by the rock rheologic differentiation, coupling of mechanics and chemistry, and inversion of tectonic regime.

  11. Thermal and compositional stratification of the inner core

    CERN Document Server

    Labrosse, Stéphane

    2014-01-01

    The improvements on the knowledge of the seismic structure of the inner core and the complexities thereby revealed ask for a dynamical origin. Sub-solidus convection was one of the early suggestions to explain the seismic anisotropy but requires an unstable density gradient either from thermal or compositional origin, or both. Temperature and composition profiles in the inner core are computed using a unidimensional model of core evolution including diffusion in the inner core and fractional crystallization at the the inner core boundary (ICB). The thermal conductivity of the core has been recently revised upwardly and, moreover, found increasing with depth. Values of the heat flow across the core mantle boundary (CMB) sufficient to maintain convection in the whole outer core are not sufficient to make the temperature in the inner core super-isentropic and therefore prone to thermal instability. An unreasonably high CMB heat flow is necessary to this end. The compositional stratification results from a compet...

  12. The structure of the core NuRD repression complex provides insights into its interaction with chromatin.

    Science.gov (United States)

    Millard, Christopher J; Varma, Niranjan; Saleh, Almutasem; Morris, Kyle; Watson, Peter J; Bottrill, Andrew R; Fairall, Louise; Smith, Corinne J; Schwabe, John W R

    2016-04-21

    The NuRD complex is a multi-protein transcriptional corepressor that couples histone deacetylase and ATP-dependent chromatin remodelling activities. The complex regulates the higher-order structure of chromatin, and has important roles in the regulation of gene expression, DNA damage repair and cell differentiation. HDACs 1 and 2 are recruited by the MTA1 corepressor to form the catalytic core of the complex. The histone chaperone protein RBBP4, has previously been shown to bind to the carboxy-terminal tail of MTA1. We show that MTA1 recruits a second copy of RBBP4. The crystal structure reveals an extensive interface between MTA1 and RBBP4. An EM structure, supported by SAXS and crosslinking, reveals the architecture of the dimeric HDAC1:MTA1:RBBP4 assembly which forms the core of the NuRD complex. We find evidence that in this complex RBBP4 mediates interaction with histone H3 tails, but not histone H4, suggesting a mechanism for recruitment of the NuRD complex to chromatin.

  13. Wavelet-Based Adaptive Solvers on Multi-core Architectures for the Simulation of Complex Systems

    Science.gov (United States)

    Rossinelli, Diego; Bergdorf, Michael; Hejazialhosseini, Babak; Koumoutsakos, Petros

    We build wavelet-based adaptive numerical methods for the simulation of advection dominated flows that develop multiple spatial scales, with an emphasis on fluid mechanics problems. Wavelet based adaptivity is inherently sequential and in this work we demonstrate that these numerical methods can be implemented in software that is capable of harnessing the capabilities of multi-core architectures while maintaining their computational efficiency. Recent designs in frameworks for multi-core software development allow us to rethink parallelism as task-based, where parallel tasks are specified and automatically mapped into physical threads. This way of exposing parallelism enables the parallelization of algorithms that were considered inherently sequential, such as wavelet-based adaptive simulations. In this paper we present a framework that combines wavelet-based adaptivity with the task-based parallelism. We demonstrate good scaling performance obtained by simulating diverse physical systems on different multi-core and SMP architectures using up to 16 cores.

  14. Clinical utility of complex mutations in the core promoterand proximal precore regions of the hepatitis B virusgenome

    Institute of Scientific and Technical Information of China (English)

    Young Min Park

    2015-01-01

    The core promoter and proximal precore regions arethe most complex portions of the hepatitis B virus(HBV) genome. These regions cooperatively regulateviral replication and differentially regulate the synthesisof the viral proteins E, core, and X. Multiple mutationsin these regions are associated with the persistencyof viral infection and the development of cirrhosis andhepatocellular carcinoma (HCC). In South Korea, nearlyall HBVs are classified as HBV genotype C2; the majorityof these viruses have the basal core promoter doublemutation, a precore stop mutation, or both. Thesemutations may play a role in the alteration of viral andclinical features, and abundant and complex mutationsare particularly prevalent in the core promoter andproximal precore regions. We previously demonstratedthat the accumulation of ≥ 6 mutations at eight keynucleotides located in these regions (G1613A, C1653T,T1753V, A1762T, G1764A, A1846T, G1896A, andG1899A) is a useful marker to predict the developmentof HCC regardless of advanced liver disease. In addition,certain mutation combinations were predominant incases with ≥ 4 mutations. In cases with ≤ 5 mutations,a low Hepatitis B e antigen titer (〈 35 signal to noiseratio) was indicative of HCC risk. Viral mutation data ofthe single HBV genotype C2 suggest that the combinedeffect of the number and pattern of mutations in thecore promoter and proximal precore regions is helpful inpredicting HCC risk.

  15. SKS anisotropy on a dense broadband array over the Ruby Mountains Metamorphic Core Complex, Nevada

    Science.gov (United States)

    Golos, E. M.; Litherland, M.; Klemperer, S. L.

    2012-12-01

    The Ruby Mountains metamorphic core complex (RMCC), located in the Basin-and-Range Province in northeastern Nevada, is thought to have formed by some combination of low-angle detachment faulting, lateral crustal flow, and vertical diapirism. We deployed a 50-station densely-spaced passive seismic array from June 2010 through June 2012, as part of the Earthscope Flexible Array campaign. We were particularly interested in determining whether two layers of anisotropy are distinguishable, as this could imply the existence of discrete crustal and mantle strain fabrics, and potentially provide insight into local flow involved in the formation of the RMCC. We analyzed SKS splitting using the SplitLab program (Wüstefeld et al., 2008, Comp. Geosci. 34, 515) to calculate fast-axis direction, Φ, and time delay, δt, of events with magnitude ≥ 5.50 at distances of 90 to 130 degrees on 35 of our broadband seismic stations. Approximately ten such events were used per station. The mean delay time found was 0.8 s with a standard deviation of 0.28 s, and the mean fast-axis azimuthal direction was -70.1 degrees with a standard deviation of 19 degrees. We did not find evidence of two-layer anisotropy beneath the Ruby Mountains: mean splitting times within and beyond the RMCC are well within one standard deviation of each other, and average fast directions show no obvious trend within the RMCC. Either there is no significant additional crustal strain associated with the RMCC formation; or, the strain direction is identical to that of regional mantle flow; or, most likely, our data quality is insufficient to resolve crustal anisotropy superimposed on mantle anisotropy with a potentially similar fast direction. However, a systematic counterclockwise rotation of fast-axis direction across our array—the four easternmost stations (D03, D02, B17, and C18) have a mean Φ = -40.5 degrees, whereas the four westernmost stations (D05, B01, B02, and C02) have a mean Φ = -79.5 degrees

  16. Grafted block complex coacervate core micelles and their effect on protein adsorption on silica and polystyrene.

    Science.gov (United States)

    Brzozowska, Agata M; de Keizer, Arie; Norde, Willem; Detrembleur, Christophe; Cohen Stuart, Martien A

    2010-07-01

    We have studied the formation and the stability of grafted block complex coacervate core micelles (C3Ms) in solution and the influence of grafted block C3M coatings on the adsorption of the proteins beta-lactoglobulin, bovine serum albumin, and lysozyme. The C3Ms consist of a grafted block copolymer PAA(21)-b-PAPEO(14) (poly(acrylic acid)-b-poly(acrylate methoxy poly(ethylene oxide)), with a negatively charged PAA block and a neutral PAPEO block and a positively charged homopolymer P2MVPI (poly(N-methyl 2-vinyl pyridinium iodide). In solution, these C3Ms partly disintegrate at salt concentrations between 50 and 100 mM NaCl. Adsorption of C3Ms and proteins has been studied with fixed-angle optical reflectometry, at salt concentrations ranging from 1 to 100 mM NaCl. In comparison with the adsorption of PAA(21)-b-PAPEO(14) alone adsorption of C3Ms significantly increases the amount of PAA(21)-b-PAPEO(14) on the surface. This results in a higher surface density of PEO chains. The stability of the C3M coatings and their influence on protein adsorption are determined by the composition and the stability of the C3Ms in solution. A C3M-PAPEO(14)/P2MVPI(43) coating strongly suppresses the adsorption of all proteins on silica and polystyrene. The reduction of protein adsorption is the highest at 100 mM NaCl (>90%). The adsorbed C3M-PAPEO(14)/P2MVPI(43) layer is partly removed from the surface upon exposure to an excess of beta-lactoglobulin solution, due to formation of soluble aggregates consisting of beta-lactoglobulin and P2MVPI(43). In contrast, C3M-PAPEO(14)/P2MVPI(228) which has a fivefold longer cationic block enhances adsorption of the negatively charged proteins on both surfaces at salt concentrations above 1 mM NaCl. A single PAA(21)-b-PAPEO(14) layer causes only a moderate reduction of protein adsorption.

  17. Exploring the GluR2 ligand-binding core in complex with the bicyclical AMPA analogue (S)-4-AHCP

    DEFF Research Database (Denmark)

    Nielsen, Bettina B; Pickering, Darryl S; Greenwood, Jeremy R;

    2005-01-01

    The X-ray structure of the ionotropic GluR2 ligand-binding core (GluR2-S1S2J) in complex with the bicyclical AMPA analogue (S)-2-amino-3-(3-hydroxy-7,8-dihydro-6H-cyclohepta[d]-4-isoxazolyl)propionic acid [(S)-4-AHCP] has been determined, as well as the binding pharmacology of this construct...

  18. Formation of metamorphic core complexes in non-over-thickened continental crust: A case study of Liaodong Peninsula (East Asia)

    OpenAIRE

    Wang, Kun; Burov, Evgueni; Gumiaux, Charles; Chen, Yan; Lu, Gang; Mezri, Leila; Zhao, Liang

    2015-01-01

    International audience; Pre-thickened hot orogenic crust is often considered a necessary condition for the formation of continental metamorphic core complexes (MCCs). However, the discovery of MCCs in the Liaodong Peninsula, where the crust has a normal thickness (~ 35 km), challenges the universality of this scenario. Therefore, we implement a series of 2-D numerical thermo-mechanical modeling experiments in which we investigate the conditions of MCC formation in normal crusts, as well as th...

  19. Sloan Great Wall as a complex of superclusters with collapsing cores

    Science.gov (United States)

    Einasto, Maret; Lietzen, Heidi; Gramann, Mirt; Tempel, Elmo; Saar, Enn; Liivamägi, Lauri Juhan; Heinämäki, Pekka; Nurmi, Pasi; Einasto, Jaan

    2016-10-01

    Context. The formation and evolution of the cosmic web is governed by the gravitational attraction of dark matter and antigravity of dark energy (cosmological constant). In the cosmic web, galaxy superclusters or their high-density cores are the largest objects that may collapse at present or during the future evolution. Aims: We study the dynamical state and possible future evolution of galaxy superclusters from the Sloan Great Wall (SGW), the richest galaxy system in the nearby Universe. Methods: We calculated supercluster masses using dynamical masses of galaxy groups and stellar masses of galaxies. We employed normal mixture modelling to study the structure of rich SGW superclusters and search for components (cores) in superclusters. We analysed the radial mass distribution in the high-density cores of superclusters centred approximately at rich clusters and used the spherical collapse model to study their dynamical state. Results: The lower limit of the total mass of the SGW is approximately M = 2.5 × 1016 h-1 M⊙. Different mass estimators of superclusters agree well, the main uncertainties in masses of superclusters come from missing groups and clusters. We detected three high-density cores in the richest SGW supercluster (SCl 027) and two in the second richest supercluster (SCl 019). They have masses of 1.2 - 5.9 × 1015 h-1 M⊙ and sizes of up to ≈60 h-1 Mpc. The high-density cores of superclusters are very elongated, flattened perpendicularly to the line of sight. The comparison of the radial mass distribution in the high-density cores with the predictions of spherical collapse model suggests that their central regions with radii smaller than 8 h-1 Mpc and masses of up to M = 2 × 1015 h-1 M⊙ may be collapsing. Conclusions: The rich SGW superclusters with their high-density cores represent dynamically evolving environments for studies of the properties of galaxies and galaxy systems.

  20. Multi-frequency Studies of Massive Cores with Complex Spatial and Kinematic Structures

    CERN Document Server

    Pirogov, L E; Zinchenko, I I; Zemlyanukha, P M; Patoka, O N; Thomasson, M

    2016-01-01

    Five regions of massive star formation have been observed in various molecular lines in the frequency range $\\sim 85-89$ GHz. The studied regions possess dense cores, which host young stellar objects. The physical parameters of the cores are estimated, including kinetic temperatures ($\\sim 20-40$ K), sizes of the emitting regions ($\\sim 0.1-0.6$ pc), and virial masses ($\\sim 40-500 M_{\\odot}$). Column densities and abundances of various molecules are calculated in the local thermodynamical equilibrium approximation. The core in 99.982+4.17, associated with the weakest IRAS source, is characterized by reduced molecular abundances. Molecular line widths decrease with increasing distance from the core centers ($b$). For $b\\ga 0.1$~pc, the dependences $\\Delta V(b)$ are close to power laws ($\\propto b^{-p}$), where $p$ varies from $\\sim 0.2$ to $\\sim 0.5$, depending on the object. In four cores, the asymmetries of the optically thick HCN(1--0) and HCO$^+$(1--0) lines indicate systematic motions along the line of s...

  1. Results of a Second-generation Constrained Condylar Prosthesis in Complex Primary and Revision Total Knee Arthroplasty: A Mean 5.5-Year Follow-up

    Institute of Scientific and Technical Information of China (English)

    Chen-Yi Ye; De-Ting Xue; Shuai Jiang; Rong-Xin He

    2016-01-01

    Background:The application of second-generation constrained condylar knee (CCK) prostheses has not been widely studied.This retrospective study was carried out to evaluate the clinical and radiographic outcomes of a second-generation CCK prosthesis for complex primary or revision total knee arthroplasty (TKA).Methods:In total,51 consecutive TKAs (47 patients) were performed between June 2003 and June 2013 using second-generation modular CCK prostheses.The follow-up was conducted at 3rd day,1st,6th,and 12th months postoperatively and later annually.Anteroposterior (AP),lateral,skyline,and long-standing AP radiographs of the affected knees were taken.The Hospital for Special Surgery (HSS) Knee Score,the Knee Society Knee Score (KSKS),the Knee Society Function Score (KSFS),and range of motion (ROM) were also recorded.Heteroscedastic two-tailed Student's t-tests were used to compare the HSS score and the Knee Society score between primary and revision TKAs.A value ofP < 0.05 was considered statistically significant.Results:Four knees (two patients) were lost to follow-up,and 47 knees (31 primary TKAs and 16 revision TKAs) had a mean follow-up time of 5.5 years.The mean HSS score improved from 51.1 ± 15.0 preoperatively to 85.3 ± 8.4 points at the final follow-up (P < 0.05).Similar results were observed in terms of the KSKS and KSFS,which improved from 26.0 ± 13.0 to 80.0 ± 12.2 and from 40.0 ± 15.0 to 85.0 ± 9.3 points,respectively (P < 0.05).No significant difference in the HSS,KSKS,KSFS,or ROM was found between primary and revision TKAs (P> 0.05).Two complications were observed in the revision TKA group (one intraoperative distal femur fracture and one recurrence of infection) while one complication (infection) was observed in the primary TKA group.No prosthesis loosening,joint dislocation,patella problems,tibial fracture,or nerve injury were observed.Radiolucent lines were observed in 4% of the knees without progressive osteolysis

  2. Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.

    Science.gov (United States)

    Krull, Sandra; Thyberg, Johan; Björkroth, Birgitta; Rackwitz, Hans-Richard; Cordes, Volker C

    2004-09-01

    The vertebrate nuclear pore complex (NPC) is a macromolecular assembly of protein subcomplexes forming a structure of eightfold radial symmetry. The NPC core consists of globular subunits sandwiched between two coaxial ring-like structures of which the ring facing the nuclear interior is capped by a fibrous structure called the nuclear basket. By postembedding immunoelectron microscopy, we have mapped the positions of several human NPC proteins relative to the NPC core and its associated basket, including Nup93, Nup96, Nup98, Nup107, Nup153, Nup205, and the coiled coil-dominated 267-kDa protein Tpr. To further assess their contributions to NPC and basket architecture, the genes encoding Nup93, Nup96, Nup107, and Nup205 were posttranscriptionally silenced by RNA interference (RNAi) in HeLa cells, complementing recent RNAi experiments on Nup153 and Tpr. We show that Nup96 and Nup107 are core elements of the NPC proper that are essential for NPC assembly and docking of Nup153 and Tpr to the NPC. Nup93 and Nup205 are other NPC core elements that are important for long-term maintenance of NPCs but initially dispensable for the anchoring of Nup153 and Tpr. Immunogold-labeling for Nup98 also results in preferential labeling of NPC core regions, whereas Nup153 is shown to bind via its amino-terminal domain to the nuclear coaxial ring linking the NPC core structures and Tpr. The position of Tpr in turn is shown to coincide with that of the nuclear basket, with different Tpr protein domains corresponding to distinct basket segments. We propose a model in which Tpr constitutes the central architectural element that forms the scaffold of the nuclear basket.

  3. Oceanic core complexes in the Philippine Sea: results from Japan's extended continental shelf mapping

    Science.gov (United States)

    Ohara, Y.; Yoshida, T.; Nishizawa, A.

    2013-12-01

    The United Nations Commission on the Limits of the Continental Shelf (CLCS) issued its recommendations on Japan's extended continental shelf in April 2012, confirming Japan's rights over the vast areas within the Philippine Sea and Pacific Plates. Japan submitted information on the limits of its continental shelf beyond the EEZ to the CLCS on November 2008, which was the result of 25 years of nation's continental shelf survey project since 1983, involving all of Japan's agency relevant to geosciences. The huge geological and geophysical data obtained through the project give the scientists unprecedented opportunity to study the geology and tectonics of the Philippine Sea and Pacific Plates. In this contribution, we show such an example from the Philippine Sea Plate, relevant to the global mid-ocean ridge problem. Oceanic core complexes (OCC) are dome-shaped bathymetric highs identified in mid-ocean ridges, interpreted as portions of the lower crust and/or upper mantle denuded via low-angle detachment faulting. OCCs are characterized morphologically by axis-normal striations (corrugations, or mullion structure) on the dome, and exposures of mantle peridotite and/or lower crustal gabbro. A strikingly giant OCC (named 'Godzilla Megamullion') was discovered in the Parece Vela Basin by the continental shelf survey project in 2001. Godzilla Megamullion is morphologically the largest OCC in the world, consisting mainly of fertile mantle peridotite along its entire length of over 125 km. Following its discovery in 2001, several academic cruises investigated the structure in detail, providing numerous important findings relevant to mid-ocean ridge tectono-magmatic processes and Philippine Sea evolution, including the slow- to ultraslow-spreading environment for denudation of the detachment fault (< 2.5 cm/y) and associated decreasing degree of partial melting of the peridotites towards the termination of Godzilla Megamullion. In addition to Godzilla Megamullion, several

  4. Structural and functional aspects of winged-helix domains at the core of transcription initiation complexes.

    Science.gov (United States)

    Teichmann, Martin; Dumay-Odelot, Hélène; Fribourg, Sébastien

    2012-01-01

    The winged helix (WH) domain is found in core components of transcription systems in eukaryotes and prokaryotes. It represents a sub-class of the helix-turn-helix motif. The WH domain participates in establishing protein-DNA and protein-protein-interactions. Here, we discuss possible explanations for the enrichment of this motif in transcription systems.

  5. Making Sense and Facing Tensions: An Investigation of Core Practice Complexities

    Science.gov (United States)

    Neel, Michael A.

    2017-01-01

    Recently, scholars have called for a practice-based framework for teacher education and some have argued more narrowly for a framework built around "core practices of teaching." These efforts, in part, are intended to make teacher education practice public and available for collective improvement. The purpose of this paper is to…

  6. Special Sm core complex functions in assembly of the U2 small nuclear ribonucleoprotein of Trypanosoma brucei.

    Science.gov (United States)

    Preusser, Christian; Palfi, Zsofia; Bindereif, Albrecht

    2009-08-01

    The processing of polycistronic pre-mRNAs in trypanosomes requires the spliceosomal small ribonucleoprotein complexes (snRNPs) U1, U2, U4/U6, U5, and SL, each of which contains a core of seven Sm proteins. Recently we reported the first evidence for a core variation in spliceosomal snRNPs; specifically, in the trypanosome U2 snRNP, two of the canonical Sm proteins, SmB and SmD3, are replaced by two U2-specific Sm proteins, Sm15K and Sm16.5K. Here we identify the U2-specific, nuclear-localized U2B'' protein from Trypanosoma brucei. U2B'' interacts with a second U2 snRNP protein, U2-40K (U2A'), which in turn contacts the U2-specific Sm16.5K/15K subcomplex. Together they form a high-affinity, U2-specific binding complex. This trypanosome-specific assembly differs from the mammalian system and provides a functional role for the Sm core variation found in the trypanosomal U2 snRNP.

  7. Syn-extensional plutonism and peak metamorphism in the albion-raft river-grouse creek metamorphic core complex

    Science.gov (United States)

    Strickland, A.; Miller, E.L.; Wooden, J.L.; Kozdon, R.; Valley, J.W.

    2011-01-01

    The Cassia plutonic complex (CPC) is a group of variably deformed, Oligocene granitic plutons exposed in the lower plate of the Albion-Raft River- Grouse Creek (ARG) metamorphic core complex of Idaho and Utah. The plutons range from granodiorite to garnet-bearing, leucogranite, and during intrusion, sillimanite- grade peak metamorphism and ductile attenuation occurred in the country rocks and normal-sense, amphibolite-grade deformation took place along the Middle Mountain shear zone. U-Pb zircon geochronology from three variably deformed plutons exposed in the lower plate of the ARG metamorphic core complex revealed that each zircon is comprised of inherited cores (dominantly late Archean) and Oligocene igneous overgrowths. Within each pluton, a spread of concordant ages from the Oligocene zircon overgrowths is interpreted as zircon recycling within a long-lived magmatic system. The plutons of the CPC have very low negative whole rock ??Nd values of -26 to -35, and initial Sr values of 0.714 to 0.718, consistent with an ancient, crustal source. Oxygen isotope ratios of the Oligocene zircon overgrowths from the CPC have an average ??18O value of 5.40 ?? 0.63 permil (2SD, n = 65) with a slight trend towards higher ??18O values through time. The ??18O values of the inherited cores of the zircons are more variable at 5.93 ?? 1.51 permil (2SD, n = 29). Therefore, we interpret the plutons of the CPC as derived, at least in part, from melting Archean crust based on the isotope geochemistry. In situ partial melting of the exposed Archean basement that was intruded by the Oligocene plutons of the CPC is excluded as the source for the CPC based on field relationships, age and geochemistry. Correlations between Ti and Hf concentrations in zircons from the CPC suggest that the magmatic system may have become hotter (higher Ti concentration in zircon) and less evolved (lower Hf in zircon concentration) through time. Therefore, the CPC represents prolonged or episodic magmatism

  8. Structural insights into yeast histone chaperone Hif1: a scaffold protein recruiting protein complexes to core histones.

    Science.gov (United States)

    Liu, Hejun; Zhang, Mengying; He, Wei; Zhu, Zhongliang; Teng, Maikun; Gao, Yongxiang; Niu, Liwen

    2014-09-15

    Yeast Hif1 [Hat1 (histone acetyltransferase 1)-interacting factor], a homologue of human NASP (nuclear autoantigenic sperm protein), is a histone chaperone that is involved in various protein complexes which modify histones during telomeric silencing and chromatin reassembly. For elucidating the structural basis of Hif1, in the present paper we demonstrate the crystal structure of Hif1 consisting of a superhelixed TPR (tetratricopeptide repeat) domain and an extended acid loop covering the rear of TPR domain, which represent typical characteristics of SHNi-TPR [Sim3 (start independent of mitosis 3)-Hif1-NASP interrupted TPR] proteins. Our binding assay indicates that Hif1 could bind to the histone octamer via histones H3 and H4. The acid loop is shown to be crucial for the binding of histones and may also change the conformation of the TPR groove. By binding to the core histone complex Hif1 may recruit functional protein complexes to modify histones during chromatin reassembly.

  9. CoreFlow: a computational platform for integration, analysis and modeling of complex biological data.

    Science.gov (United States)

    Pasculescu, Adrian; Schoof, Erwin M; Creixell, Pau; Zheng, Yong; Olhovsky, Marina; Tian, Ruijun; So, Jonathan; Vanderlaan, Rachel D; Pawson, Tony; Linding, Rune; Colwill, Karen

    2014-04-04

    A major challenge in mass spectrometry and other large-scale applications is how to handle, integrate, and model the data that is produced. Given the speed at which technology advances and the need to keep pace with biological experiments, we designed a computational platform, CoreFlow, which provides programmers with a framework to manage data in real-time. It allows users to upload data into a relational database (MySQL), and to create custom scripts in high-level languages such as R, Python, or Perl for processing, correcting and modeling this data. CoreFlow organizes these scripts into project-specific pipelines, tracks interdependencies between related tasks, and enables the generation of summary reports as well as publication-quality images. As a result, the gap between experimental and computational components of a typical large-scale biology project is reduced, decreasing the time between data generation, analysis and manuscript writing. CoreFlow is being released to the scientific community as an open-sourced software package complete with proteomics-specific examples, which include corrections for incomplete isotopic labeling of peptides (SILAC) or arginine-to-proline conversion, and modeling of multiple/selected reaction monitoring (MRM/SRM) results. CoreFlow was purposely designed as an environment for programmers to rapidly perform data analysis. These analyses are assembled into project-specific workflows that are readily shared with biologists to guide the next stages of experimentation. Its simple yet powerful interface provides a structure where scripts can be written and tested virtually simultaneously to shorten the life cycle of code development for a particular task. The scripts are exposed at every step so that a user can quickly see the relationships between the data, the assumptions that have been made, and the manipulations that have been performed. Since the scripts use commonly available programming languages, they can easily be

  10. CoreFlow: A computational platform for integration, analysis and modeling of complex biological data

    DEFF Research Database (Denmark)

    Pasculescu, Adrian; Schoof, Erwin; Creixell, Pau

    2014-01-01

    between data generation, analysis and manuscript writing. CoreFlow is being released to the scientific community as an open-sourced software package complete with proteomics-specific examples, which include corrections for incomplete isotopic labeling of peptides (SILAC) or arginine-to-proline conversion...... provides programmers with a framework to manage data in real-time. It allows users to upload data into a relational database (MySQL), and to create custom scripts in high-level languages such as R, Python, or Perl for processing, correcting and modeling this data. CoreFlow organizes these scripts...... into project-specific pipelines, tracks interdependencies between related tasks, and enables the generation of summary reports as well as publication-quality images. As a result, the gap between experimental and computational components of a typical large-scale biology project is reduced, decreasing the time...

  11. Knotty-centrality: finding the connective core of a complex network.

    Directory of Open Access Journals (Sweden)

    Murray Shanahan

    Full Text Available A network measure called knotty-centrality is defined that quantifies the extent to which a given subset of a graph's nodes constitutes a densely intra-connected topologically central connective core. Using this measure, the knotty centre of a network is defined as a sub-graph with maximal knotty-centrality. A heuristic algorithm for finding subsets of a network with high knotty-centrality is presented, and this is applied to previously published brain structural connectivity data for the cat and the human, as well as to a number of other networks. The cognitive implications of possessing a connective core with high knotty-centrality are briefly discussed.

  12. Evolution of complex organic molecules in hot molecular cores: Synthetic spectra at (sub-)mm wavebands

    CERN Document Server

    Choudhury, Rumpa; Stéphan, Gwendoline; Bergin, Edwin A; Möller, Thomas; Schmiedeke, Anika; Zernickel, Alexander

    2015-01-01

    Hot molecular cores (HMCs) are intermediate stages of high-mass star formation and are also known for their rich emission line spectra at (sub-)mm wavebands. The observed spectral feature of HMCs such as total number of emission lines and associated line intensities are also found to vary with evolutionary stages. We developed various 3D models for HMCs guided by the evolutionary scenarios proposed by recent empirical and modeling studies. We then investigated the spatio-temporal variation of temperature and molecular abundances in HMCs by consistently coupling gas-grain chemical evolution with radiative transfer calculations. We explored the effects of varying physical conditions on molecular abundances including density distribution and luminosity evolution of the central protostar(s). The time-dependent temperature structure of the hot core models provides a realistic framework for investigating the spatial variation of ice mantle evaporation as a function of evolutionary timescales. With increasing protos...

  13. Structural performance of complex core systems for FRP-balsa composite sandwich bridge decks

    OpenAIRE

    Osei-Antwi, Michael

    2014-01-01

    Based on current fiber-reinforced polymer (FRP) composite construction principles, FRP decks fall into two categories: pultruded decks and sandwich decks. Sandwich decks comprise face sheets and either honeycombs or foams reinforced with internal FRP webs for shear resistance. The honeycomb structure and the webs cause debonding between the upper face sheets and the core due to the uneven support of the former. An alternative material that has high shear capacity and can provide uniform ...

  14. Sloan Great Wall as a complex of superclusters with collapsing cores

    CERN Document Server

    Einasto, Maret; Gramann, Mirt; Tempel, Elmo; Saar, Enn; Liivamägi, Lauri Juhan; Heinämäki, Pekka; Nurmi, Pasi; Einasto, Jaan

    2016-01-01

    In the cosmic web, galaxy superclusters or their high-density cores are the largest objects that may collapse at present or during the future evolution. We study the dynamical state and possible future evolution of galaxy superclusters from the Sloan Great Wall (SGW), the richest galaxy system in the nearby Universe. We calculated supercluster masses using dynamical masses of galaxy groups and stellar masses of galaxies. We employed normal mixture modelling to study the structure of rich SGW superclusters and search for components (cores) in superclusters. We analysed the radial mass distribution in the high-density cores of superclusters centred approximately at rich clusters and used the spherical collapse model to study their dynamical state. We found that the lower limit of the total mass of the SGW is approximately $M = 2.5\\times~10^{16}h^{-1}M_\\odot$. Different mass estimators of superclusters agree well, the main uncertainties in masses of superclusters come from missing groups and clusters. We detecte...

  15. Revising Translations

    DEFF Research Database (Denmark)

    Rasmussen, Kirsten Wølch; Schjoldager, Anne

    2011-01-01

    out by specialised revisers, but by staff translators, who revise the work of colleagues and freelancers on an ad hoc basis. Corrections are mostly given in a peer-to-peer fashion, though the work of freelancers and inexperienced in-house translators is often revised in an authoritative (nonnegotiable...

  16. Exploring molecular complexity with ALMA (EMoCA): Detection of three new hot cores in Sagittarius B2(N)

    Science.gov (United States)

    Bonfand, M.; Belloche, A.; Menten, K. M.; Garrod, R. T.; Müller, H. S. P.

    2017-08-01

    Context. The Sagittarius B2 molecular cloud contains several sites forming high-mass stars. Sgr B2(N) is one of its main centers of activity. It hosts several compact and ultra-compact HII regions, as well as two known hot molecular cores (Sgr B2(N1) and Sgr B2(N2)) in the early stage of the high-mass star formation process, where complex organic molecules (COMs) are detected in the gas phase. Aims: Our goal is to use the high sensitivity of the Atacama Large Millimeter/submillimeter Array (ALMA) to characterize the hot core population in Sgr B2(N) and thereby shed new light on the star formation process in this star-forming region. Methods: We use a complete 3 mm spectral line survey conducted with ALMA to search for faint hot cores in the Sgr B2(N) region. The chemical composition of the detected sources and the column densities are derived by modeling the whole spectra under the assumption of local thermodynamic equilibrium. Population diagrams are constructed to fit rotational temperatures. Integrated intensity maps are produced to derive the peak position and fit the size of each molecule's emission distribution. The kinematic structure of the hot cores is investigated by analyzing the line wing emission of typical outflow tracers. The H2 column densities are computed from ALMA and SMA continuum emission maps. Results: We report the discovery of three new hot cores in Sgr B2(N) that we call Sgr B2(N3), Sgr B2(N4), and Sgr B2(N5). The three sources are associated with class II methanol masers, well known tracers of high-mass star formation, and Sgr B2(N5), also with a UCHII region. Their H2 column densities are found to be between approximately 16 and 36 times lower than the one of the main hot core Sgr B2(N1). The spectra of these new hot cores have spectral line densities of 11 up to 31 emission lines per GHz above the 7σ level, assigned to 22-25 molecules plus 13-20 less abundant isotopologs. We derive rotational temperatures of approximately 140-180 K for

  17. The Distribution of Complex Organic Molecules in the Orion KL Molecular Core

    Science.gov (United States)

    Kuan, Yi-Jehng; Hsu, Yu-Sen; Charnley, Steven B.; Wang, Kuo-Song

    2011-01-01

    We conducted high angular-resolution observations toward the massive star-forming region Orion KL at 1.3 mm using the Submillimeter Array (SMA). Spectral emission from twelve complex organic molecules was simultaneously imaged. We discuss the distinct chemical characteristics among four sub- regions in Orion KL by comparing the spatial distributions and fractional abundances of these complex molecules. These observations will allow us to test and constrain chemical models of interstellar organic synthesis.

  18. Final Environmental Impact Statement for the Stillwater National Wildlife Refuge Complex Comprehensive Conservation Plan and Boundary Revision

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on Stillwater NWR Complex for the next 15 years. This plan outlines the Complex vision and...

  19. Systematic technology evaluation program for SiC/SiC composite-based accident-tolerant LWR fuel cladding and core structures: Revision 2015

    Energy Technology Data Exchange (ETDEWEB)

    Katoh, Yutai [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Terrani, Kurt A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-08-01

    Fuels and core structures in current light water reactors (LWR’s) are vulnerable to catastrophic failure in severe accidents as unfortunately evidenced by the March 2011 Fukushima Dai-ichi Nuclear Power Plant Accident. This vulnerability is attributed primarily to the rapid oxidation kinetics of zirconium alloys in a water vapor environment at very high temperatures. Zr alloys are the primary material in LWR cores except for the fuel itself. Therefore, alternative materials with reduced oxidation kinetics as compared to zirconium alloys are sought to enable enhanced accident-tolerant fuels and cores.

  20. An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome

    Energy Technology Data Exchange (ETDEWEB)

    Foerster, Friedrich [Department of Structural Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried (Germany); Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California at San Francisco, San Francisco (United States); Lasker, Keren [Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California at San Francisco, San Francisco (United States); Blavatnik School of Computer Science, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv (Israel); Beck, Florian; Nickell, Stephan [Department of Structural Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried (Germany); Sali, Andrej [Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California at San Francisco, San Francisco (United States); Baumeister, Wolfgang, E-mail: baumeist@biochem.mpg.de [Department of Structural Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried (Germany)

    2009-10-16

    The 26S proteasome is the most downstream element of the ubiquitin-proteasome pathway of protein degradation. It is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP consists of 6 AAA-ATPases and at least 13 non-ATPase subunits. Based on a cryo-EM map of the 26S proteasome, structures of homologs, and physical protein-protein interactions we derive an atomic model of the AAA-ATPase-CP sub-complex. The ATPase order in our model (Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rpt5) is in excellent agreement with the recently identified base-precursor complexes formed during the assembly of the RP. Furthermore, the atomic CP-AAA-ATPase model suggests that the assembly chaperone Nas6 facilitates CP-RP association by enhancing the shape complementarity between Rpt3 and its binding CP alpha subunits partners.

  1. An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome.

    Science.gov (United States)

    Förster, Friedrich; Lasker, Keren; Beck, Florian; Nickell, Stephan; Sali, Andrej; Baumeister, Wolfgang

    2009-10-16

    The 26S proteasome is the most downstream element of the ubiquitin-proteasome pathway of protein degradation. It is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP consists of 6 AAA-ATPases and at least 13 non-ATPase subunits. Based on a cryo-EM map of the 26S proteasome, structures of homologs, and physical protein-protein interactions we derive an atomic model of the AAA-ATPase-CP sub-complex. The ATPase order in our model (Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rpt5) is in excellent agreement with the recently identified base-precursor complexes formed during the assembly of the RP. Furthermore, the atomic CP-AAA-ATPase model suggests that the assembly chaperone Nas6 facilitates CP-RP association by enhancing the shape complementarity between Rpt3 and its binding CP alpha subunits partners.

  2. The effects of lower crustal strength and preexisting midcrustal shear zones on the formation of continental core complexes and low-angle normal faults

    KAUST Repository

    Wu, Guangliang

    2016-08-22

    To investigate the formation of core complexes and low-angle normal faults, we devise thermomechanical simulations on a simplified wedge-like orogenic hinterland that has initial topography, Moho relief, and a preexisting midcrustal shear zone that can accommodate shear at very low angles (<20°). We mainly vary the strength of the lower crust and the frictional strength of the preexisting midcrustal shear zone. We find that the strength of the lower crust and the existence and strength of a preexisting shear zone significantly affect the formation and evolution of core complexes. With increasing lower crustal strength, we recognize varying extensional features with decreasing exhumation rate: these are characterized by bivergent metamorphic massifs, classic Cordilleran metamorphic core complexes, multiple consecutive core complexes (or boudinage structures), and a flexural core complex underlined by a large subsurface low-angle detachment fault with a small convex curvature. Topographic loading and mantle buoyancy forces, together with divergent boundaries, drive a regional lower crustal flow that leads to the exhumation of the lower crust where intensive upper crustal faulting induces strong unloading. The detachment fault is a decoupling zone that accommodates large displacement and accumulates sustained shear strain at very low angle between upper and lower crust. Though the regional stress is largely Andersonian, we find non-Andersonian stress in regions adjacent to the preexisting shear zone and those with high topographic gradient. Our new models provide a view that is generally consistent with geological and geophysical observations on how core complexes form and evolve.

  3. Recommendations for a first Core Outcome Measurement set for complex regional PAin syndrome Clinical sTudies (COMPACT).

    Science.gov (United States)

    Grieve, Sharon; Perez, Roberto Sgm; Birklein, Frank; Brunner, Florian; Bruehl, Stephen; Harden, R Norman; Packham, Tara; Gobeil, Francois; Haigh, Richard; Holly, Janet; Terkelsen, Astrid; Davies, Lindsay; Lewis, Jennifer; Thomassen, Ilona; Connett, Robyn; Worth, Tina; Vatine, Jean-Jacques; McCabe, Candida S

    2017-02-04

    Complex Regional Pain Syndrome (CRPS) is a persistent pain condition that remains incompletely understood and challenging to treat. Historically, a wide range of different outcome measures have been used to capture the multidimensional nature of CRPS. This has been a significant limiting factor in the advancement of our understanding of the mechanisms and management of CRPS.In 2013, an international consortium of patients, clinicians, researchers and industry representatives was established, to develop and agree on a minimum core set of standardised outcome measures for use in future CRPS clinical research, including but not limited to clinical trials within adult populationsThe development of a core measurement set was informed through workshops and supplementary work, using an iterative consensus process. 'What is the clinical presentation and course of CRPS, and what factors influence it?' was agreed as the most pertinent research question that our standardised set of patient-reported outcome measures should be selected to answer. The domains encompassing the key concepts necessary to answer the research question were agreed as: pain, disease severity, participation and physical function, emotional and psychological function, self efficacy, catastrophizing and patient's global impression of change. The final core measurement set included the optimum generic or condition-specific patient-reported questionnaire outcome measures, which captured the essence of each domain, and one clinician reported outcome measure to capture the degree of severity of CRPS. The next step is to test the feasibility and acceptability of collecting outcome measure data using the core measurement set in the CRPS population internationally.

  4. Revealing and tuning the core, structure, properties and function of polymer micelles with lanthanide-coordination complexes.

    Science.gov (United States)

    Wang, Junyou; Groeneveld, Andrea; Oikonomou, Maria; Prusova, Alena; Van As, Henk; van Lent, Jan W M; Velders, Aldrik H

    2016-01-07

    Controlling self-assembly processes is of great interest in various fields where multifunctional and tunable materials are designed. We here present the versatility of lanthanide-complex-based micelles (Ln-C3Ms) with tunable coordination structures and corresponding functions (e.g. luminescence and magnetic relaxation enhancement). Micelles are prepared by charge-driven self-assembly of a polycationic-neutral diblock copolymer and anionic coordination complexes formed by Ln(III) ions and the bis-ligand L2EO4, which contains two dipicolinic acid (DPA) ligand groups (L) connected by a tetra-ethylene oxide spacer (EO4). By varying the DPA/Ln ratio, micelles are obtained with similar size but with different stability, different aggregation numbers and different oligomeric and polymeric lanthanide(III) coordination structures in the core. Electron microscopy, light scattering, luminescence spectroscopy and magnetic resonance relaxation experiments provide an unprecedented detailed insight into the core structures of such micelles. Concomitantly, the self-assembly is controlled such that tunable luminescence or magnetic relaxation with Eu-C3Ms, respectively, Gd-C3Ms is achieved, showing potential for applications, e.g. as contrast agents in (pre)clinical imaging. Considering the various lanthanide(III) ions have unique electron configurations with specific physical chemical properties, yet very similar coordination chemistry, the generality of the current coordination-structure based micellar design shows great promise for development of new materials such as, e.g., hypermodal agents.

  5. Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex.

    Science.gov (United States)

    Fukumura, Takuma; Makino, Fumiaki; Dietsche, Tobias; Kinoshita, Miki; Kato, Takayuki; Wagner, Samuel; Namba, Keiichi; Imada, Katsumi; Minamino, Tohru

    2017-08-01

    The bacterial flagellar type III export apparatus, which is required for flagellar assembly beyond the cell membranes, consists of a transmembrane export gate complex and a cytoplasmic ATPase complex. FlhA, FlhB, FliP, FliQ, and FliR form the gate complex inside the basal body MS ring, although FliO is required for efficient export gate formation in Salmonella enterica. However, it remains unknown how they form the gate complex. Here we report that FliP forms a homohexameric ring with a diameter of 10 nm. Alanine substitutions of conserved Phe-137, Phe-150, and Glu-178 residues in the periplasmic domain of FliP (FliPP) inhibited FliP6 ring formation, suppressing flagellar protein export. FliO formed a 5-nm ring structure with 3 clamp-like structures that bind to the FliP6 ring. The crystal structure of FliPP derived from Thermotoga maritia, and structure-based photo-crosslinking experiments revealed that Phe-150 and Ser-156 of FliPP are involved in the FliP-FliP interactions and that Phe-150, Arg-152, Ser-156, and Pro-158 are responsible for the FliP-FliO interactions. Overexpression of FliP restored motility of a ∆fliO mutant to the wild-type level, suggesting that the FliP6 ring is a functional unit in the export gate complex and that FliO is not part of the final gate structure. Copurification assays revealed that FlhA, FlhB, FliQ, and FliR are associated with the FliO/FliP complex. We propose that the assembly of the export gate complex begins with FliP6 ring formation with the help of the FliO scaffold, followed by FliQ, FliR, and FlhB and finally FlhA during MS ring formation.

  6. Revision of the Ambrysus guttatipennis Stål species complex (Heteroptera: Naucoridae: Cryphocricinae) with the descriptions of six new species from Mexico.

    Science.gov (United States)

    Reynoso-Velasco, Daniel; Sites, Robert W

    2016-08-22

    The Ambrysus guttatipennis Stål species complex is revised and includes A. arizonus La Rivers, A. bispinus La Rivers, A. drakei La Rivers, A. guttatipennis Stål, A. mexicanus Montandon, and A. mormon Montandon. Six new species that belong to this complex are described from Mexico: A. ayoyolin n. sp., A. bowlesi n. sp., A. contrerasi n. sp., A. itsipatsari n. sp., A. noveloi n. sp., and A. veracruzanus n. sp. The subspecies A. mormon australis La Rivers, A. m. heidemanni Montandon, and A. m. minor La Rivers are proposed as junior synonyms of A. m. mormon Montandon. A supplemental redescription of A. guttatipennis Stål based on the holotype is provided and a lectotype of A. mexicanus Montandon is designated. Ambrysus arizonus is newly recorded from Mexico and A. bispinus from Guatemala. Most of the species in this complex occur only in Mexico, although A. arizonus and A. mormon are distributed in Mexico and the United States, and A. bispinus is distributed in Mexico and Guatemala. Ambrysus mormon has the widest distribution of any species in this complex. Features uniting these species are related to male genitalia and structures associated with male and female genitalia.

  7. A self-assembled complex with a titanium(IV) catecholate core as a potential bimodal contrast agent.

    Science.gov (United States)

    Dehaen, Geert; Eliseeva, Svetlana V; Kimpe, Kristof; Laurent, Sophie; Vander Elst, Luce; Muller, Robert N; Dehaen, Wim; Binnemans, Koen; Parac-Vogt, Tatjana N

    2012-01-01

    A ditopic chelating ligand (H(6)4) that bears catechol and diethylenetriamine-N,N,N',N'',N''-pentaacetate (DTPA) has been designed and shown to specifically bind lanthanide(III) ions at the DTPA core ([Ln(H(2)4)(H(2)O)](-)) and further self-assemble with titanium(IV), thereby giving rise to the formation of a supramolecular metallostar complex with a lanthanide(III)-to-titanium(IV) ratio of 3:1, [(Ln4)(3)Ti(H(2)O)(3)](5-) (Ln=La, Eu, Gd). The efficacy of the metallostar complex as a potential bimodal optical/magnetic resonance imaging (MRI) agent has been evaluated. Nuclear magnetic relaxation dispersion (NMRD) measurements for the [(Gd4)(3)Ti(H(2)O)(3)](5-) complex have demonstrated an enhanced r(1) relaxivity that corresponds to 36.9 s(-1) mM(-1) per metallostar molecule at 20 MHz and 310 K, which is a result of a decreased tumbling rate. The ability of the complex to bind to human serum albumin (HSA) was also examined by relaxometric measurements. In addition, upon UV irradiation the [(Gd4)(3)Ti(H(2)O)(3)](5-) complex exhibits broad-band green emission in the range 400-750 nm with a maximum at 490 nm. Taking into account the high relaxivity and luminescence properties, the [(Gd4)(3)Ti(H(2)O)(3)](5-) complex is a good lead compound for the development of efficient bimodal contrast agents.

  8. Full-length core sequence dependent complex-type glycosylation of hepatitis C virus E2 glycoprotein

    Institute of Scientific and Technical Information of China (English)

    Li-Xin Zhu; Jing Liu; Ying-Chun Li; Yu-Ying Kong; Caroline Staib; Gerd Sutter; Yuan Wang; Guang-Di Li

    2002-01-01

    AIM: To study HCV polyprotein processing is important forthe understanding of the natural history of HCV and thedesign of vaccines against HCV. The purpose of this studyis to investigate the affection of context sequences onhepatitis C virus (HCV) E2 processingMETHODS: HCV genes of different lengths were expressedand compared in vaccinia virus/T7 system with homologouspatient serum S94 and mouse anti-serum ME2116 raisedagainst E. coli-derived E2 peptide, respectively.Deglycosylation analysis and GNA (Galanthus nivalus )lectin binding assay were performed to study the post-translational processing of the expressed products.RESULTS: E2 glycoproteins with different molecular weights( ~ 75kDa end ~ 60kDa) were detected using S94 and ME2116,respectively. Deglycosylation analysis showed that thisdifference was mainly due to different glycosylation. Endo Hresistance and its failure to bind to GNA lectin demonstratedthat the higher molecular weight form (75kDa) of E2 wascomplex-type glycosylated, which was readily recognized byhomologous patient serum S94. Expression of complex-typeglycosylated E2 could not be detected in all of the core-truncated constructs tested, but readily detected inconstructs encoding full-length core sequences.CONCLUSION: The upstream conserved full-length corecoding sequence was required for the production of E2glycoproteins carrying complex-type N-glycans whichreacted strongly with homologous patient serum andtherefore possibly represented more mature forms of E2. Ascomplex-type N-glycans indicated modification by Golgienzymes, the results suggest that the presence of full-lengthcore might be critical for E1/E2 complex to leave ER. Ourdata may contribute to a better understanding of theprocessing of HCV structural proteins as well as HCVmorphogenesis.

  9. Analysis of magnetotelluric profile data from the Ruby Mountains metamorphic core complex and southern Carlin Trend region, Nevada

    Science.gov (United States)

    Wannamaker, Philip E.; Doerner, William M.; Stodt, John A.; Sodergen, Timothy L.; Rodriguez, Brian D.

    2002-01-01

    We have collected about 150 magnetotelluric (MT) soundings in northeastern Nevada in the region of the Ruby Mountains metamorphic core complex uplift and southern Carlin mineral trend, in an effort to illuminate controls on core complex evolution and deposition of world-class gold deposits. The region has experienced a broad range of tectonic events including several periods of compressional and extensional deformation, which have contributed to the total expression of electrical resistivity. Most of the soundings are in three east-west profiles across increasing degrees of core uplift to the north (Bald Mountain, Harrison Pass and Secret Pass latitudes). Two shorter lines cross a prominent east-west structure to the north of the northern profile. MT impedance tensor and vertical magnetic field rotations imply a N-NNE average regional geoelectric strike, similar to surface geologic trends. Model resistivity cross sections were derived using a 2-D inversion algorithm, which damps departures of model parameters from an a priori structure, emphasizing the transverse magnetic (TM) mode and vertical magnetic field data. Geological interpretation of the resistivity combines previous seismic, potential field and isotope models, structural and petrological models for regional compression and extension, and detailed structural/stratigraphic interpretations incorporating drilling for petroleum and mineral exploration. To first order, the resistivity structure is one of a moderately conductive, Phanerozoic sedimentary section fundamentally disrupted by intrusion and uplift of resistive crystalline rocks. Late Devonian and early Mississippian shales of the Pilot and Chainman Formations together form an important conductive marker sequence in the stratigraphy and show pronounced increases in conductance (conductivity-thickness product) from east to west. These increases in conductance are attributed to graphitization caused by Elko-Sevier era compressional shear deformation and

  10. Síntese e reatividade de complexos platina-trifenilestibina: uma revisão bibliográfica Synthesis and reactivity of triphenylstibine-platinum complexes: a bibliographic revision

    Directory of Open Access Journals (Sweden)

    Roberto Santos Barbiéri

    2006-07-01

    Full Text Available This article deals with synthesis and reactivity of complexes with triphenylstibine (SbPh3 as the ligand. A comparative study of analogous complexes of triphenylphosphine (PPh3 and triphenylarsine (AsPh3 with platinum in the oxidation states zero, two and four is included. The bibliographic revision includes publications since 1936, when the first Pt(II complex with triphenylstibine was described.

  11. On the occurrence of the fireworm Eurythoe complanata complex (Annelida, Amphinomidae in the Mediterranean Sea with an updated revision of the alien Mediterranean amphinomids

    Directory of Open Access Journals (Sweden)

    Andrés Arias

    2013-09-01

    Full Text Available The presence of two species within the Eurythoe complanata complex in the Mediterranean Sea is reported, as well as their geographical distributions. One species, Eurythoe laevisetis, occurs in the eastern and central Mediterranean, likely constituting the first historical introduction to the Mediterranean Sea and the other, Eurythoe complanata, in both eastern and Levantine basins. Brief notes on their taxonomy are also provided and their potential pathways for introduction to the Mediterranean are discussed. A simplified key to the Mediterranean amphinomid genera and species of Eurythoe and Linopherus is presented plus an updated revision of the alien amphinomid species reported previously from the Mediterranean Sea. A total of five exotic species have been included; information on their location, habitat, date of introduction and other relevant features is also provided.

  12. Core-Shell Soy Protein-Soy Polysaccharide Complex (Nano)particles as Carriers for Improved Stability and Sustained Release of Curcumin.

    Science.gov (United States)

    Chen, Fei-Ping; Ou, Shi-Yi; Tang, Chuan-He

    2016-06-22

    Using soy protein isolate (SPI) and soy-soluble polysaccharides (SSPS) as polymer matrixes, this study reported a novel process to fabricate unique core-shell complex (nano)particles to perform as carriers for curcumin (a typical poorly soluble bioactive). In the process, curcumin-SPI nanocomplexes were first formed at pH 7.0 and then coated by SSPS. At this pH, the core-shell complex was formed in a way the SPI nanoparticles might be incorporated into the interior of SSPS molecules without distinctly affecting the size and morphology of particles. The core-shell structure was distinctly changed by adjusting pH from 7.0 to 4.0. At pH 4.0, SSPS was strongly bound to the surface of highly aggregated SPI nanoparticles, and as a consequence, much larger complexes were formed. The bioaccessibility of curcumin in the SPI-curcumin complexes was unaffected by the SSPS coating. However, the core-shell complex formation greatly improved the thermal stability and controlled release properties of encapsulated curcumin. The improvement was much better at pH 4.0 than that at pH 7.0. All of the freeze-dried core-shell complex preparations exhibited good redispersion behavior. The findings provide a simple approach to fabricate food-grade delivery systems for improved water dispersion, heat stability, and even controlled release of poorly soluble bioactives.

  13. Density functional theory study of model complexes for the revised nitrate reductase active site in Desulfovibrio desulfuricans NapA.

    Science.gov (United States)

    Hofmann, Matthias

    2009-09-01

    [Mo(SSCH3)(S2C2(CH3)2)2](x) complexes with charges x between -3 and +3 were investigated by density functional theory computations as minimal nitrate reductase active-site models. The strongly reduced species (x = -2, -3) exist preferentially as pentacoordinate sulfo complexes separated from a thiolate anion. The oxidized extremes (x > 0) clearly prefer hexacoordinate complexes with an eta(2)-MeSS ligand. Among the neutral and especially for the singly negatively charged species structures with eta(2)-MeSS and eta(1)-MeSS ligands are energetically close to the sulfo methyl sulfide complex without SS bonding. For x = -1 the three isomers lie in a 1.5 kcal mol(-1) energy range. Putative mechanistic pathways for nitrate reduction from the literature were investigated computationally: (1) reduction at a pentacoordinate sulfo complex, (2) reduction at the ligand, and (3) reduction at the molybdenum center with an R-S-S ligand. All three pathways could be traced at least for some overall charges but no definite conclusion can be drawn about the mechanism. Complexes with larger dithiolato ligands were also computed in order to model the tricyclic metallopterin framework more accurately: the first heterocyclus (5,6-dihydro-2H-pyran) stabilizes the nitrate complex and the molybdenum oxo product complex by approximately 10 kcal mol(-1) and also reduces the activation barrier (by approximately 5 kcal mol(-1)). The effect of the second (1,2,3,4-tetrahydropyrazin) and third heterocyclus (2-amino-3H-pyrimidin-4-one) on the relative energies is relatively small. For bigger models derived from an experimental protein structure, nitrate reduction at a persulfo molybdenum(IV) complex fragment (mechanism 3) is clearly favored over the oxidation of a molybdenum-bound sulfur atom (mechanism 2). Mechanism 1 could not be investigated for the big models but seems the least favorable on the basis of the results from smaller models.

  14. The fistulectome: a new device for treatment of complex anal fistulas by "Core-Out" fistulectomy.

    Science.gov (United States)

    Tasci, Ihsan

    2003-11-01

    In an attempt to improve the quality of life of patients with high anal fistula, we developed a new mechanical device, a "fistulectome," which excises the fistula tract in a totally controlled manner, particularly useful in the treatment of high anal fistulas. The "fistulectomy set" consists of a flexible shaft, cannulation and fixation guides, an incisor mouth, and a handle, which is simultaneously used for motor housing. The principle of the treatment is to excise approximately 2-mm thickness of the fistula tract circumferentially, which in fact is a "coring-out" procedure. The fistula tract is likewise transformed into a cylindrical cavity encircled by healthy tissue. This is achieved by the fistulectomy set, consisting of a flexible shaft, cannulation and fixation guides, an incisor mouth, and a handle simultaneously used for motor housing. Between March 2001 and April 2002, a total of 13 consecutive patients with anal fistula underwent excision of fistula tracts. All patients except one had previously been operated on for anal fistula. The distribution of fistulas was as follows: transsphincteric, six patients; suprasphincteric, three patients; extrasphincteric, three patients; multiple, one patient. Mean follow-up was 13.4 (range 7.5-18) months. Gas incontinence in one patient, soiling in one patient, and recurrence in one patient was observed. No recurrences, stool, or gas incontinence were observed in ten patients. Excision of fistula tract performed by the recently developed fistulectome is a minimally invasive, safe, and effective method to be considered in the treatment of anal fistula. The results obtained up to date were encouraging, although the patient number was limited.

  15. Formation of core-shell structured complex microparticles during fabrication of magnetorheological elastomers and their magnetorheological behavior

    Science.gov (United States)

    Wang, Yonghong; Zhang, Xinru; Chung, Kyungho; Liu, Chengcen; Choi, Seung-Bok; Choi, Hyoung Jin

    2016-11-01

    To improve mechanical and magnetorheological properties of magnetorheological elastomers (MREs), a facile method was used to fabricate high-performance MREs which consisted of the core-shell complex microparticles with an organic-inorganic network structure dispersed in an ethylene propylene diene rubber. In this work, the proposed magnetic complex microparticles were in situ formed during MREs fabrication as a result of strong interaction between matrix and CIPs using carbon black as a connecting point. The morphology of both isotropic (i-MREs) and anisotropic MREs (a-MREs) was observed by scanning electron microscope (SEM). The effects of carbonyl iron particle (CIP) volume content on mechanical properties and hysteresis loss of MREs were investigated. The effects of CIP volume content on the shear storage modulus, MR effect and loss tangent were studied using a modified dynamic mechanical analyzer under applied magnetic field strengths. The results showed that the orientation effect became more pronounced with increasing CIPs in the a-MREs, whereas CIPs distributed uniformly in the i-MREs. The tensile strength, tear strength and elongation at break decreased with increasing CIP content up to 40 vol.%, while the hardness increased. It is worth noting that the tensile strength of i-MREs and a-MREs containing 40 vol.% CIPs still had high mechanical properties as a result of good compatibility between complex microparticles and rubber matrix. The MR performance of shear storage modulus and damping properties of MREs increased remarkably with CIP content due to strong dipole-dipole interaction of complex microparticles. Besides, the hysteresis loss increased with increasing CIP content as a result of magnetic field induced interfacial sliding between complex microparticles.

  16. Core regulatory network motif underlies the ocellar complex patterning in Drosophila melanogaster

    Science.gov (United States)

    Aguilar-Hidalgo, D.; Lemos, M. C.; Córdoba, A.

    2015-03-01

    During organogenesis, developmental programs governed by Gene Regulatory Networks (GRN) define the functionality, size and shape of the different constituents of living organisms. Robustness, thus, is an essential characteristic that GRNs need to fulfill in order to maintain viability and reproducibility in a species. In the present work we analyze the robustness of the patterning for the ocellar complex formation in Drosophila melanogaster fly. We have systematically pruned the GRN that drives the development of this visual system to obtain the minimum pathway able to satisfy this pattern. We found that the mechanism underlying the patterning obeys to the dynamics of a 3-nodes network motif with a double negative feedback loop fed by a morphogenetic gradient that triggers the inhibition in a French flag problem fashion. A Boolean modeling of the GRN confirms robustness in the patterning mechanism showing the same result for different network complexity levels. Interestingly, the network provides a steady state solution in the interocellar part of the patterning and an oscillatory regime in the ocelli. This theoretical result predicts that the ocellar pattern may underlie oscillatory dynamics in its genetic regulation.

  17. Synthesis and design of organic light-emitting devices containing lanthanide-cored complexes

    Science.gov (United States)

    Phelan, Gregory D.; Carlson, Brenden; Lawson, Rhys; Rowe, Daniel; Allen, Kolby; Dalton, Larry; Jiang, Xuezhong; Kim, Joo H.; Jen, Alex K.

    2004-02-01

    There is a considerable interest in the use of metal centered materials as a light source in the growing field of organic light emitting devices (OLED's). In these devices, a polymeric host matrix containing either a carbazole type polymer or polyfluorene derivatives is used to help facilitate energy transfer to the luminophore. We have shown that by using a gadolinium complex that consist of three equivalents of a chelated dibenzoylmethane b-diketone ligand and one equivalent of a phenanthroline type ligand as a component in the host matrix, the performance of a double layer type OLED is improved. We have studied OLED systems that contain tris chelated europium compounds that contain three equivalents of partially fluorinated β-diketone type ligands and an equivalent of a phenanthroline type ligand. In these devices, the external efficiency has shown a 30-fold increase. We have also shown there is an increase for Osmium based OLED's that use the gadolinium complex as part of the polymer matrix. In these devices, the maximum quantum efficiency increased from 2.1% to a value of 3.8%.

  18. Multistate Redox Switching and Near-Infrared Electrochromism Based on a Star-Shaped Triruthenium Complex with a Triarylamine Core

    Science.gov (United States)

    Tang, Jian-Hong; He, Yan-Qin; Shao, Jiang-Yang; Gong, Zhong-Liang; Zhong, Yu-Wu

    2016-01-01

    A star-shaped cyclometalated triruthenium complex 2(PF6)n (n = 3 and 4) with a triarylamine core was synthesized, which functions as a molecular switch with five well-separated redox states in both solution and film states. The single-crystal X-ray structure of 2(PF6)3 is presented. This complex displays four consecutive one-electron redox waves at +0.082, +0.31, +0.74, and +1.07 V vs Ag/AgCl. In each redox state, it shows significantly different NIR absorptions with λmax of 1590 nm for 24+, 1400 nm for 25+, 1060 nm for 26+, and 740 nm for 27+, respectively. Complex 24+ shows a single-line EPR signal at g = 2.060, while other redox states are all EPR inactive. The spin density distributions and NIR absorptions in different redox states were rationalized by DFT and TDDFT calculations. A vinyl-substituted triruthenium analogous 3(PF6)4 was prepared, which was successfully polymerized on ITO glass electrode surfaces by reductive electropolymerization. The obtained poly-3n+/ITO film was characterized by FTIR, AFM, and SEM analysis. It shows four well-defined redox couples and reversible multistate NIR electrochromism. In particular, a contrast ratio (ΔT%) up to 63% was achieved at the optic telecommunication wavelength (1550 nm). PMID:27731404

  19. Perplexing cooperative folding and stability of a low-sequence complexity, polyproline 2 protein lacking a hydrophobic core.

    Science.gov (United States)

    Gates, Zachary P; Baxa, Michael C; Yu, Wookyung; Riback, Joshua A; Li, Hui; Roux, Benoît; Kent, Stephen B H; Sosnick, Tobin R

    2017-02-28

    The burial of hydrophobic side chains in a protein core generally is thought to be the major ingredient for stable, cooperative folding. Here, we show that, for the snow flea antifreeze protein (sfAFP), stability and cooperativity can occur without a hydrophobic core, and without α-helices or β-sheets. sfAFP has low sequence complexity with 46% glycine and an interior filled only with backbone H-bonds between six polyproline 2 (PP2) helices. However, the protein folds in a kinetically two-state manner and is moderately stable at room temperature. We believe that a major part of the stability arises from the unusual match between residue-level PP2 dihedral angle bias in the unfolded state and PP2 helical structure in the native state. Additional stabilizing factors that compensate for the dearth of hydrophobic burial include shorter and stronger H-bonds, and increased entropy in the folded state. These results extend our understanding of the origins of cooperativity and stability in protein folding, including the balance between solvent and polypeptide chain entropies.

  20. Oxygen-evolving Activity in Photosystem Ⅱ Core Complex of Photosynthetic Membrane in the Presence of Native Lipid

    Institute of Scientific and Technical Information of China (English)

    YANG,Zhen-Le(阳振乐); WANG,Ze-Neng(王则能); LI,Liang-Bi(李良璧); KUANG,Ting-Yun(匡廷云)

    2002-01-01

    The techniques of oxygen electrode polarography and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the involvement of digalactosyl diacylglycerol (DGDG) in functional and structural roles in the photosysten Ⅱ core com-plex (PSⅡCC). It was shown that DGDG exhibited the ability to stimulate the oxygen evolution in PSⅡCC, which was accompanied by the changes in the strucctures of PSⅡCC proteins.Tne results revealed that there existed hydrogen-bonding interactions between DGDG molecules and PSⅡCC proteins. It is most likely that the sites of PSⅡCC interaction with DGDG are in the extrinsic protein of 33 kDa.

  1. High expression of PI3K core complex genes is associated with poor prognosis in chronic lymphocytic leukemia

    DEFF Research Database (Denmark)

    Kristensen, Louise; Kielsgaard Kristensen, Thomas; Abildgaard, Niels;

    2015-01-01

    Chronic lymphocytic leukemia (CLL) is the most common leukemia among adults in the Western world. Autophagy is a highly conserved process in eukaryotic cells. In CLL autophagy is involved in mediating the effect of chemotherapy but the role of autophagy in CLL pathogenesis remains unknown....... In the present study, we used real-time RT-PCR to analyze expression of the PIK3C3, PIK3R4, and BECN1 genes. These genes encode the components of the PI3K core complex, which is central to initiation of autophagy. A consecutive series of 149 well-characterized CLL cases from Region of Southern Denmark were...... on the role of autophagy in CLL, and they may further represent targets of treatment....

  2. The effect of PEO block lengths on the size and stability of complex coacervate core micelles.

    Science.gov (United States)

    Adams, Dave J; Rogers, Sue H; Schuetz, Peter

    2008-06-15

    We report on a series of polyion complexes from mixtures of poly(ethylene oxide)-block-poly(N,N-diethylaminoethylmethacrylate) (PEO-PDEAMA) and poly(ethylene oxide)-block-poly(aspartic acid) (PEO-PAsp). As expected, the micelle size, polydispersity and stability are dependant on the relative and absolute lengths of the polyelectrolyte chains. However, we also demonstrate that whilst the length of the charged polyelectrolyte blocks is important, the length of the PEO chains is an equally relevant variable in determining both the size and stability of the final micelles as well as the degree of charge neutralisation at which micellisation occurs. We also show that the kinetics of formation can result in very different stability of the final micelles.

  3. Single hepatitis-B virus core capsid binding to individual nuclear pore complexes in Hela cells.

    Science.gov (United States)

    Lill, Yoriko; Lill, Markus A; Fahrenkrog, Birthe; Schwarz-Herion, Kyrill; Paulillo, Sara; Aebi, Ueli; Hecht, Bert

    2006-10-15

    We investigate the interaction of hepatitis B virus capsids lacking a nuclear localization signal with nuclear pore complexes (NPCs) in permeabilized HeLa cells. Confocal and wide-field optical images of the nuclear envelope show well-spaced individual NPCs. Specific interactions of capsids with single NPCs are characterized by extended residence times of capsids in the focal volume which are characterized by fluorescence correlation spectroscopy. In addition, single-capsid-tracking experiments using fast wide-field fluorescence microscopy at 50 frames/s allow us to directly observe specific binding via a dual-color colocalization of capsids and NPCs. We find that binding occurs with high probability on the nuclear-pore ring moiety, at 44 +/- 9 nm radial distance from the central axis.

  4. Using cognitive behavioural therapy with complex cases: using the therapeutic relationship to change core beliefs.

    Science.gov (United States)

    Binnie, James

    2012-07-01

    Cognitive Behavioural Therapy (CBT) is often perceived as a manualised, symptom focused, surface level approach. This article aims to reflect on working with complex clinical presentations and explore how third wave CBT can be effectively integrated into standard cognitive behavioural interventions. To achieve these aims, a case study of a CBT assessment and treatment is presented. The interventions used are described in detail. The focus changes from the more traditional symptom-led interventions to third wave approaches based on the therapeutic relationship. When the focus was redirected towards the therapeutic relationship then real change occurred, quickly and powerfully. Reflections on the process are discussed and the overall approach used was evaluated with an action plan developed to enhance future clinical practice. It is hoped that this study can help CBT be viewed as a comprehensive form of psychotherapy.

  5. Conformation of the troponin core complex in the thin filaments of skeletal muscle during relaxation and active contraction.

    Science.gov (United States)

    Knowles, Andrea C; Irving, Malcolm; Sun, Yin-Biao

    2012-08-03

    Contraction of skeletal and cardiac muscles is regulated by Ca(2+) binding to troponin in the actin-containing thin filaments, leading to an azimuthal movement of tropomyosin around the filament that uncovers the myosin binding sites on actin. Here, we use polarized fluorescence to determine the orientation of the C-terminal lobe of troponin C (TnC) in skeletal muscle cells as a step toward elucidating the molecular mechanism of troponin-mediated regulation. Assuming, as shown by X-ray crystallography, that this lobe of TnC is part of a well-defined troponin domain called the IT arm, we show that the coiled coil formed by troponin components I and T makes an angle of about 55° with the thin filament axis in relaxed muscle, in contrast with previous models based on electron microscopy in which this angle is close to 0°. The E helix of TnC makes an angle of about 45° with the thin filament axis. Both the IT coiled coil and the TnC E helix tilt by about 10° on muscle activation. By combining in situ measurements of the orientation of the IT arm and regulatory domain of troponin, which together form the troponin core complex, with published intermolecular distances between thin filament components, we derive models of thin filament structure in which the IT arm of troponin holds its regulatory domain close to the actin surface. Although the structure and function of troponin regions outside the core complex remain to be characterized, the present results provide useful constraints for molecular models of the mechanism of muscle regulation. Copyright © 2012 Elsevier Ltd. All rights reserved.

  6. IGCSE core mathematics

    CERN Document Server

    Wall, Terry

    2013-01-01

    Give your core level students the support and framework they require to get their best grades with this book dedicated to the core level content of the revised syllabus and written specifically to ensure a more appropriate pace. This title has been written for Core content of the revised Cambridge IGCSE Mathematics (0580) syllabus for first teaching from 2013. ? Gives students the practice they require to deepen their understanding through plenty of practice questions. ? Consolidates learning with unique digital resources on the CD, included free with every book. We are working with Cambridge

  7. Limnephilid taxa revised by speciation traits: Rhadicoleptus, Isogamus, Melampophylax genera, Chaetopteryx rugulosa, Psilopteryx psorosa species groups, Drusus bolivari, Annitella kosciuszkii species complexes (Trichoptera: Limnephilidae

    Directory of Open Access Journals (Sweden)

    Oláh, J.

    2015-06-01

    redescription of Drusus bolivari (McLachlan, 1876, with species status resurrection of D. estrellensis (McLachlan, 1884 stat. restit., with description of five new species: D. carmenae Oláh, sp. nov., D. gonzalezi Oláh, sp. nov., D. grafi Oláh, sp. nov., D. gredosensis Oláh, sp. nov., D. jesusi Oláh, sp. nov., D. pyrenensis Oláh & Coppa, sp. nov. Genus Isogamus is revised with description of two new species: I. baloghi Oláh, sp. nov., I. balinti Oláh, sp. nov. Melampophylax genus revised with one new species cluster: M. nepos, with two new species descriptions: M. keses Coppa & Oláh, sp. nov. M. szczesnyorum Oláh & Chvojka, sp. nov., with three new species status: M. banaticus Botosaneanu, 1995 stat. nov., M. gutinicus Botosaneanu, 1995 stat. nov., M. triangulifera Botosaneanu, 1957 stat. nov. Rhadicoleptus genus revised with redescription of R. alpestris (Kolenati, 1848, with three new species status: R. macedonicus Botosaneanu & Riedel, 1965 stat. nov. R. meridiocarpaticus Botosaneanu & Riedel, 1965 stat. nov. R. sylvanocarpaticus Botosaneanu & Riedel, 1965 stat. nov., with one species status resurrection: R. spinifer (McLachlan, 1875 stat. restit. Based on paramere evolution the Rhadicoleptus genus is transferred from the tribe Limnephilini to Stenophylacini. Annitella kosciuszkii new species complex has been erected and revised with redescription of A. chomiacensis (Dziędzielewicz, 1908, A. lateroproducta (Botosaneanu, 1952, with one species status resurrection: A. kosciuszkii Klapálek, 1907 stat. restit., with description of a new species: A. wolosatka Oláh &Szczęsny, sp. nov., with two new synonyms: A. dziedzielewiczi Schmid, 1952 synonym of A. kosciuszkii. syn. nov., A. transylvanica Murgoci, 1957 synonym of A. kosciuszkii. syn. nov. Chaetopteryx rugulosa species group revised with description of five new species: C. balcanica Oláh, sp. nov., C. karima Oláh, sp. nov., C. kozarensis Oláh, sp. nov., C. psunjensis Oláh, sp. nov., C. tompa Oláh, sp. nov

  8. Trans-ethyl methyl ether in space - A new look at a complex molecule in selected hot core regions

    CERN Document Server

    Fuchs, G W; Giesen, T F; Wyrowski, F

    2005-01-01

    An extensive search for the complex molecule trans-ethyl methyl ether towards several hot core regions has been performed. Using the IRAM 30m telescope and the SEST 15m we looked at several frequencies where trans-ethyl methyl ether has strong transitions, as well as lines which are particularly sensitive to the physical conditions in which the molecule can be found. We included G34.26, NGC6334(I), Orion KL, and W51e2 which have previously been proven to have a rich chemistry of complex molecules. Our observations cannot confirm the tentative Orion KL detection made by Charnley et al. (2001) within their stated column density limits, but we confirm the existence of the trans-ethyl methyl ether towards W51e2 with a column density of 2x10^14 cm-2. The dimethyl ether/methanol ratio of 0.6 as well as the newly found ethyl methyl ether/ethanol ratio of 0.13 indicate relative high abundances of ethers toward W51e2. Furthermore, the observation of ethyl methyl ether also confirms the importance of ethanol as a grain...

  9. Structural analysis and deformation characteristics of the Yingba metamorphic core complex, northwestern margin of the North China craton, NE Asia

    Science.gov (United States)

    Yin, Congyuan; Zhang, Bo; Han, Bao-Fu; Zhang, Jinjiang; Wang, Yang; Ai, Sheng

    2017-01-01

    The presence of the Yingba (Yinggete-Bagemaode) metamorphic core complex (MCC) is confirmed near the Sino-Mongolian border in China. We report its structural evolution and the rheological features of ductile shear zones within this complex. Three deformations (Ds, Dm, and Db) since the Late Jurassic are identified. Ds is characterized by ductile structures that resulted from early NW-oriented, low-angle, extensional ductile shearing. Dm is associated with partial melting and magmatic diapirism, which accelerated the formation of the dome-like geometry of the Yingba MCC. Synchronously with or slightly subsequently to Ds and Dm, the Yingba MCC was subjected to brittle, extensional faulting (Db), which was accompanied by the exhumation of the lower crust and the formation of supracrustal basins. The ductile shearing (Ds) developed under greenschist-to amphibolite-facies metamorphic conditions (400-650 °C), as indicated by microstructures in quartz and feldspar, quartz [c] axis fabrics, and two-feldspar geothermometry. The mean kinematic vorticity estimates of 48-62% show a pure shear-preferred flow during Ds. The Yingba MCC provides an excellent sample that recorded an intermediate to high temperature shearing, which also implies the widely extensional regime in northeastern Asia at that time.

  10. Characteristic differences in the formation of complex coacervate core micelles from neodymium and zinc-based coordination polymers.

    Science.gov (United States)

    Yan, Yun; Besseling, Nicolaas A M; de Keizer, Arie; Stuart, Martien A Cohen

    2007-05-31

    In this paper we compare the formation of complex coacervate core micelles (C3Ms) from two different tricompontent mixtures, namely neodymium, the bisligand L2EO4 and the poly(cation)-block-poly(neutral) diblock copolymer P2MVP41-b-PEO205, and zinc, L2EO4 and P2MVP41-b-PEO205 mixed systems. Three sets of titration experiments were carried out for each system: (i) titration of diblock copolymer P2MVP41-b-PEO205 with the stoichiometric mixture of metal ions and bisligands, (ii) titration of a mixture of diblock copolymer and bisligand with metal ions, and (iii) titration of a mixture of diblock copolymer and metal ions with bisligands. In all the above three cases, micelles are found to form either in a broad range of charge ratios or in a broad range of metal/bisligand ratios. Upon addition of Nd2-(L2EO4)3 coordination polymer to P2MVP41-b-PEO205 solution, and upon addition of Nd3+ to a mixture of L2EO4 and P2MVP41-b-PEO205, micelles are found to form immediately after the first addition, whereas micelles show up in the similar zinc system only after a certain threshold Zn-(L2EO4) or Zn2+ concentration. This difference can be traced to the different structures of the Nd2-(L2EO4)3 and Zn-(L2EO4) coordination compounds. At very low concentrations, Zn-(L2EO4) are ring-like oligomers, but Nd2-(L2EO4)3 are larger networks. The network structure favors the formation of coacervate micellar core with P2MVP41-b-PEO205. Moreover, excess of Nd3+ ions will break up the C3Ms, while the same amount of Zn2+ has hardly any effect on the C3Ms. The breakdown of C3Ms by Nd3+ is due to the charge inversion of the coordination complex with increasing [Nd3+]/[L2EO4] ratio, which results in repulsive interaction between the coordination complex and the diblock copolymer, whereas no such interaction can occur in the zinc system.

  11. Revising Translations

    DEFF Research Database (Denmark)

    Rasmussen, Kirsten Wølch; Schjoldager, Anne

    2011-01-01

    out by specialised revisers, but by staff translators, who revise the work of colleagues and freelancers on an ad hoc basis. Corrections are mostly given in a peer-to-peer fashion, though the work of freelancers and inexperienced in-house translators is often revised in an authoritative (nonnegotiable......) way. Most respondents and interviewees are worried about increasing pressures on the translation market, which, combined with customers’ general lack of understanding of the translation process, mean that systematic, all-encompassing quality assurance is rarely financially viable....

  12. Revision Notes

    CERN Document Server

    Matthewson, Siobhan; Debbadi, Margaret

    2012-01-01

    Revision Notes: CCEA ICT for GCSE has been written by experienced teachers and examiners so that you can be confident that it covers only the facts and ideas you will be expected to recall and use in the exam. - Essential facts are carefully organised to make revising easier. - Exams tips show you how to avoid losing marks and get the best grade. - Check your understanding questions support you in the run-up to the exams, with answers provided free online at www.hodderplus.co.uk. This book will help you plan and pace your revision to suit your learning needs and can be integrated with other re

  13. 木犀科松林丁香复合体的分类修订%Taxonomic revision of Syringa pinetorum complex (Oleaceae)

    Institute of Scientific and Technical Information of China (English)

    陈进勇; 张佐双; 洪德元

    2008-01-01

    对松林丁香S. pinetorum W. W. Sm.复合体内各学者发表的5个新种进行了分类修订.根据居群取样、性状分析和主坐标分析结果, 圆叶丁香S. wardii W. W. Sm.、S. mairei (H. Lév.) Rehder、S. rugulosa McKelvey和川西丁香S. chuanxiensis S. Z. Qu & X. L. Chen被处理为松林丁香的异名, 其中S. mairei为新异名.此复合体只有一种, 即松林丁香.%Five species of the Syringa pinetorum complex described by previous authors are revised. Syringa wardii W. W. Sm., S. mairei (H. Lév.) Rehder, S. rugulosa McKelvey and S. chuanxiensis S. Z. Qu & X. L. Chen are treated as synonyms of S. pinetorum on the basis of population sampling, character analysis and principal coordinate analysis, and S. mairei is a new synonym. Only one variable species, S. pinetorum, is here recognized in the complex.

  14. Ethical Revision.

    Science.gov (United States)

    Jackman, Mary Kathryn

    1994-01-01

    Discusses the dilemma of how to respond to student papers advancing morally repugnant positions. Advocates conceptualizing writing as an ethical act and connecting ethics and revision. Describes briefly how three such student papers were handled. (SR)

  15. Scar revision

    Science.gov (United States)

    ... chap 21. Lorenz P, Bari AS. Scar prevention, treatment, and revision. In: Neligan PC, ed. Plastic Surgery . 3rd ed. Philadelphia, PA: Elsevier; 2013:chap 16. Read More Contracture deformity Keloids Review Date 4/14/2015 Updated ...

  16. A Practical Propositional Knowledge Base Revision Algorithm

    Institute of Scientific and Technical Information of China (English)

    陶雪红; 孙伟; 等

    1997-01-01

    This paper gives an outline of knowledge base revision and some recently presented complexity results about propostitional knowledge base revision.Different methods for revising propositional knowledge base have been proposed recently by several researchers,but all methods are intractable in the general case.For practical application,this paper presents a revision method for special case,and gives its corresponding polynomial algorithm.

  17. Miocene core complex development and coeval supradetachment basin evolution of Paros, Greece, insights from (U-Th)/He thermochronometry

    Science.gov (United States)

    Bargnesi, Evan A.; Stockli, Daniel F.; Mancktelow, Neil; Soukis, Konstantinos

    2013-06-01

    The Aegean region of Greece hosts a series of crustal-scale extensional detachment systems that have accommodated the southward retreating Hellenic subduction zone. Extension has overprinted and dissected the Alpine nappe pile and locally exhumed Cordilleran-type metamorphic core complexes. On the island of Paros, a low-angle extensional detachment fault separates metamorphic footwall rocks from an unmetamorphosed sedimentary succession of the hanging wall. Basement orthogneisses were extensionally sheared in the footwall of the detachment until after 16 Ma (zircon U-Pb age of a slightly deformed granite), but pervasive ductile deformation had ceased by 7 Ma (zircon U-Pb age of an undeformed rhyolite dike that intrudes gneisses). Apatite and zircon (U-Th)/He ages from the gneisses confirm a period of cooling at rates > 100 °C/Ma from 16 to 7 Ma. In the upper-plate, the basal sedimentary unit yields reset detrital apatite (U-Th)/He (DAHe) ages from 17 to 7 Ma and detrital zircon (U-Th)/He (DZHe) ages ranging from 270 to 18 Ma. DAHe ages from the stratigraphically higher fanglomerate units are reset to 10-7 Ma. The DZHe data have a primary thermal signature of 12-7 Ma, but preserve ages up to 113 Ma. The uppermost conglomerates exhibit completely reset DAHe ages of 15-9 Ma and reset DZHe ages from 10 to 8 Ma, with DZHe ages up to 104 Ma. Reset DAHe ages indicate late exposure of the footwall and constrain the depositional age of most sedimentary rocks on Paros to be from 14 to 7 Ma. Unreset DZHe ages preserve thermal signatures from the major Mesozoic-Tertiary tectonic events in the Aegean Region: [1] Cretaceous Pelagonian-type metamorphism; [2] Eocene peak HP metamorphism; and [3] Miocene Barrovian overprinting. Preservation of these signatures indicates long-term upper-plate recycling prior to syn-extensional deposition. The Paros supradetachment basin represents a classic inverted unroofing sequence deposited during progressive core complex exhumation in the

  18. P- T- t constraints on the development of the Doi Inthanon metamorphic core complex domain and implications for the evolution of the western gneiss belt, northern Thailand

    Science.gov (United States)

    Macdonald, A. S.; Barr, S. M.; Miller, B. V.; Reynolds, P. H.; Rhodes, B. P.; Yokart, B.

    2010-01-01

    The western gneiss belt in northern Thailand is exposed within two overlapping Cenozoic structural domains: the extensional Doi Inthanon metamorphic core complex domain located west of the Chiang Mai basin, and the Mae Ping strike-slip fault domain located west of the Tak batholith. New P- T estimates and U-Pb and 40Ar/ 39Ar age determinations from the Doi Inthanon domain show that the gneiss there records a complex multi-stage history that can be represented by a clockwise P- T- t path. U-Pb zircon and titanite dating of mylonitic calc-silicate gneiss from the Mae Wang area of the complex indicates that the paragneissic sequence experienced high-grade, medium-pressure metamorphism (M1) in the Late Triassic - Early Jurassic (ca. 210 Ma), in good agreement with previously determined zircon ages from the underlying core orthogneiss exposed on Doi Inthanon. Late Cretaceous monazite ages of 84 and 72 Ma reported previously from the core orthogneiss are attributed to a thermal overprint (M2) to upper-amphibolite facies in the sillimanite field. U-Pb zircon and monazite dating of granitic mylonite from the Doi Suthep area of the complex provides an upper age limit of 40 Ma (Late Eocene) for the early stage(s) of development of the actual core complex, by initially ductile, low-angle extensional shearing under lower amphibolite-facies conditions (M3), accompanied by near-isothermal diapiric rise and decompression melting. 40Ar/ 39Ar laserprobe dating of muscovite from both Doi Suthep and Doi Inthanon provided Miocene ages of ca. 26-15 Ma, representing cooling through the ca. 350 °C isotherm and marking late-stage development of the core complex by detachment faulting of the cover rocks and isostatic uplift of the sheared core zone and mantling gneisses in the footwall. Similarities in the thermochronology of high-grade gneisses exposed in the core complex and shear zone domains in the western gneiss belt of northern Thailand (and also in northern Vietnam, Laos, Yunnan

  19. Complex coacervate core micelles

    NARCIS (Netherlands)

    Voets, I.K.; Keizer, de A.; Cohen Stuart, M.A.

    2009-01-01

    In this review we present an overview of the literature on the co-assembly of neutral-ionic block, graft, and random copolymers with oppositely charged species in aqueous solution. Oppositely charged species include synthetic (co)polymers of various architectures, biopolymers - such as proteins, enz

  20. Complex coacervate core micelles

    NARCIS (Netherlands)

    Voets, I.K.; Keizer, de A.; Cohen Stuart, M.A.

    2009-01-01

    In this review we present an overview of the literature on the co-assembly of neutral-ionic block, graft, and random copolymers with oppositely charged species in aqueous solution. Oppositely charged species include synthetic (co)polymers of various architectures, biopolymers - such as proteins,

  1. Heterologous Production of the Photosynthetic Reaction Center and Light Harvesting 1 Complexes of the Thermophile Thermochromatium tepidum in the Mesophile Rhodobacter sphaeroides and Thermal Stability of a Hybrid Core Complex.

    Science.gov (United States)

    Jun, D; Huang, V; Beatty, J T

    2017-10-15

    The photosynthetic complexes of the thermophile Thermochromatium tepidum are of considerable interest in biohybrid solar cell applications because of the ability of thermophilic proteins to tolerate elevated temperatures. Synthetic operons encoding reaction center (RC) and light harvesting 1 (LH1) pigment-protein complexes of T. tepidum were expressed in the mesophile Rhodobacter sphaeroides The T. tepidum RC (TRC) was assembled and was found to be functional with the addition of menadione to populate the QA pocket. The production of T. tepidum LH1 (TLH1) was increased by selection of a phototrophy-capable mutant after UV irradiation mutagenesis, which yielded a hybrid RC-TLH1 core complex consisting of the R. sphaeroides RC and T. tepidum TLH1, confirmed by the absorbance peak of TLH1 at 915 nm. Affinity chromatography partial purification and subsequent sucrose gradient analysis of the hybrid RC-TLH1 core complex indicated that this core complex assembled as a monomer. Furthermore, the RC-TLH1 hybrid core complex was more tolerant of a temperature of 70°C than the R. sphaeroides RC-LH1 core complexes in both the dimeric and monomeric forms; after 1 h, the hybrid complex retained 58% of the initial starting value, compared to values of 11% and 53% for the R. sphaeroides RC-LH1 dimer and monomer forms, respectively.IMPORTANCE This work is important because it is a new approach to bioengineering of photosynthesis proteins for potential use in biophotovoltaic solar energy capture. The work establishes a proof of principle for future biohybrid solar cell applications. Copyright © 2017 American Society for Microbiology.

  2. Complement fixing hepatitis B core antigen immune complexes in the liver of patients with HBs antigen positive chronic disease.

    Science.gov (United States)

    Rizzetto, M; Bonino, F; Crivelli, O; Canese, M G; Verme, G

    1976-01-01

    One hundred and fifty-two biopsies from serologically HBsAg positive and negative patients with liver disease were studied in immunofluorescence: for the presence of the surface (HBs) and the core (HBc) antigenic determinants foeterminants of the hepatitis B virus, of immunoglobulins and complement (C) deposits, and for the capacity to fix human C. Circumstantial evidence is presented suggesting that HBc immune-complexes are a relevant feature in the establishment and progression of chronic HBSAg liver disease. C fixation by liver cells was shown in all HBC positive patients with chronic hepatitis; an active form was present in every case, except two with a persistent hepatitis, an inverse ratio of HBc to C binding fluorescence being noted between active chronic hepatitis and cirrhotic patients. HBc without C fixation was observed in only three patients in the incubation phase of infectious hepatitis. IgG deposits were often found in HBc containing, C fixing nuclei. No C binding or IgG deposits were observed in acute self-limited type B hepatitis, in serologically positive patients with normal liver or minimal histological lesions, with and without HBs cytoplasmic fluorescence in their biopsy, or in serologically negative individuals. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:1001973

  3. Planting increases the abundance and structure complexity of soil core functional genes relevant to carbon and nitrogen cycling.

    Science.gov (United States)

    Wang, Feng; Liang, Yuting; Jiang, Yuji; Yang, Yunfeng; Xue, Kai; Xiong, Jinbo; Zhou, Jizhong; Sun, Bo

    2015-09-23

    Plants have an important impact on soil microbial communities and their functions. However, how plants determine the microbial composition and network interactions is still poorly understood. During a four-year field experiment, we investigated the functional gene composition of three types of soils (Phaeozem, Cambisols and Acrisol) under maize planting and bare fallow regimes located in cold temperate, warm temperate and subtropical regions, respectively. The core genes were identified using high-throughput functional gene microarray (GeoChip 3.0), and functional molecular ecological networks (fMENs) were subsequently developed with the random matrix theory (RMT)-based conceptual framework. Our results demonstrated that planting significantly (P soils and 83.5% of microbial alpha-diversity can be explained by the plant factor. Moreover, planting had significant impacts on the microbial community structure and the network interactions of the microbial communities. The calculated network complexity was higher under maize planting than under bare fallow regimes. The increase of the functional genes led to an increase in both soil respiration and nitrification potential with maize planting, indicating that changes in the soil microbial communities and network interactions influenced ecological functioning.

  4. Reduction of protein adsorption on silica and polysulfone surfaces coated with complex coacervate core micelles with poly(vinyl alcohol) as a neutral brush forming block

    NARCIS (Netherlands)

    Brzozowska, A. M.; Zhang, Q.; de Keizer, A.; Norde, W.; Stuart, M. A. Cohen

    2010-01-01

    We have studied the formation and stability of complex coacervate core micelles (C3Ms) in solution, and the influence of C3M coatings on the adsorption of the proteins beta-lactoglobulin (beta-lac), bovine serum albumin (BSA). and lysozyme (Lsz) on silica and polysulfone surfaces. The C3M5 consist o

  5. 3-D seismic imaging of lithospheric fault-block structures, core complexes, alteration fronts, and hydrothermal systems along the Mid-Atlantic Ridge, Rainbow area

    Science.gov (United States)

    Dunn, R. A.; Arai, R.; Eason, D. E.; Canales, J. P.; Sohn, R. A.

    2016-12-01

    Oceanic lithosphere formed along slow-spreading mid-ocean ridges is structurally and compositionally heterogeneous due to spatial and temporal variations in tectonic extension and magmatic accretion processes. Sorting out the different influences requires detailed imaging of the subsurface. The MARINER seismic and geophysical mapping experiment was designed to examine seafloor spreading across an area that includes a non-transform offset of the Mid-Atlantic Ridge, 36°14'N, the site of the Rainbow core complex and its associated hydrothermal vent field. Using seismic refraction data from this experiment, we constructed three-dimensional anisotropic tomographic images of the crust and upper mantle around the Rainbow area. Approaching Rainbow along the spreading ridges from either side, the seismic images reveal the onset of a clear ridge-parallel stripe-like structures, with alternating high- and low-velocities throughout the crust, correlated with changing lower crustal thickness and the locations of large normal faults. The pattern indicates that large normal faults rotate large blocks of the entire crust during tectonic stretching. Sitting within the ridge offset, the Rainbow core complex appears to be genetically related to neighboring fault blocks, and is largely an ultramafic exposure. Relatively low seismic velocities drape the core complex, having a sharp contact with higher-velocities below. The sharp contact may demarcate alteration (to serpentinite) and cracking fronts, since also draping the core complex are corresponding regions of high seismic anisotropy and high microseismicity, indicating pervasive cracking of its upper regions. The anisotropy and seismicity funnel upwards under the vent field, presumably marking the flow paths of vent fluids that cool melt lenses found to be intruded deep below the surface. The tomographic images reveal lithospheric structures in greater detail than previously possible, and when taken together with our other

  6. Core-shell nanofibers of curcumin/cyclodextrin inclusion complex and polylactic acid: Enhanced water solubility and slow release of curcumin.

    Science.gov (United States)

    Aytac, Zeynep; Uyar, Tamer

    2017-02-25

    Core-shell nanofibers were designed via electrospinning using inclusion complex (IC) of model hydrophobic drug (curcumin, CUR) with cyclodextrin (CD) in the core and polymer (polylactic acid, PLA) in the shell (cCUR/HPβCD-IC-sPLA-NF). CD-IC of CUR and HPβCD was formed at 1:2 molar ratio. The successful formation of core-shell nanofibers was revealed by TEM and CLSM images. cCUR/HPβCD-IC-sPLA-NF released CUR slowly but much more in total than PLA-CUR-NF at pH 1 and pH 7.4 due to the restriction of CUR in the core of nanofibers and solubility improvement shown in phase solubility diagram, respectively. Improved antioxidant activity of cCUR/HPβCD-IC-sPLA-NF in methanol:water (1:1) is related with the solubility enhancement achieved in water based system. The slow reaction of cCUR/HPβCD-IC-sPLA-NF in methanol is associated with the shell inhibiting the quick release of CUR. On the other hand, cCUR/HPβCD-IC-sPLA-NF exhibited slightly higher rate of antioxidant activity than PLA-CUR-NF in methanol:water (1:1) owing to the enhanced solubility. To conclude, slow release of CUR was achieved by core-shell nanofiber structure and inclusion complexation of CUR with HPβCD provides high solubility. Briefly, electrospinning of core-shell nanofibers with CD-IC core could offer slow release of drugs as well as solubility enhancement for hydrophobic drugs.

  7. Complete structural analysis of the Upper plate of Attica metamorphic core complex (Sub-Pelagonian Zone, Internal Hellenides, Central Greece)

    Science.gov (United States)

    Diamantopoulos, A.

    2009-04-01

    Two structural plates compose the Miocene Cordillera-type core complex of Attica, separated by a km-scale detachment fault (Diamantopoulos 2005, Diamantopoulos 2006). The Upper Plate contains rocks of the Sub-Pelagonian Zone and the Neogene basin of Athens. The Lower Plate includes Neogene basins developed onto Late Cenozoic a-type metamorphic domes. This work analyzes the geometry and the kinematic path of flow of rock masses of the Sub-Pelagonian rocks from the northern parts of Penteli mountain up to the Gulf of Alkyonides. The UP comprises Permo-Triassic rocks, Triassic-Jurassic carbonates and Late Jurassic melange, Mesozoic serpentinites containing Fe-Ni rocks, occurrences of carbonates and radiolarites, Cretaceous limestones as well as Paleocene flysch. A 3D structural analysis in all the scales concludes that: a) Multiple steep- and low-angle cataclastic shear zones define the boundaries among distinctive Permo-Triassic rocks, among Triassic-Jurassic rocks and Permo-Triassic rocks, among Permo-Triassic rocks and Triassic-Jurassic rocks, among Triassic-Jurassic rocks and serpentinites, among serpentinites and Triassic-Jurassic rocks, among Triassic-Jurassic rocks and Jurassic mélange, among Jurassic mélange and Triassic-Jurassic rocks, among Triassic-Jurassic rocks and Jurassic radiolarites, among Cretaceous and Triassic-Jurassic rocks, among Triassic-Jurassic rocks and Fe-Ni rocks, among Cretaceous and Fe-Ni rocks, among Paleocene and Triassic-Jurassic rocks, among Paleocene and Permo-Triassic rocks as well as among Cretaceous and Paleocene rocks, b) Apparent omissions of intermediate lithologies throughout the entire nappe stack observed in multiple locations suggest intense non-coaxial thinning, c) A remarkable contrast in the distributed strain between the distinctive lithologies is well-recognized, dependent by the rheological and mechanical character of the rocks, d) Thrust-like geometries and macroscopic repetitions between competent and incompetent

  8. Crustal structure in the Elko-Carlin Region, Nevada, during Eocene gold mineralization: Ruby-East Humboldt metamorphic core complex as a guide to the deep crust

    Science.gov (United States)

    Howard, K.A.

    2003-01-01

    The deep crustal rocks exposed in the Ruby-East Humboldt metamorphic core complex, northeastern Nevada, provide a guide for reconstructing Eocene crustal structure ???50 km to the west near the Carlin trend of gold deposits. The deep crustal rocks, in the footwall of a west-dipping normal-sense shear system, may have underlain the Pin??on and Adobe Ranges about 50 km to the west before Tertiary extension, close to or under part of the Carlin trend. Eocene lakes formed on the hanging wall of the fault system during an early phase of extension and may have been linked to a fluid reservoir for hydrothermal circulation. The magnitude and timing of Paleogene extension remain indistinct, but dikes and tilt axes in the upper crust indicate that spreading was east-west to northwest-southeast, perpendicular to a Paleozoic and Mesozoic orogen that the spreading overprinted. High geothermal gradients associated with Eocene or older crustal thinning may have contributed to hydrothermal circulation in the upper crust. Late Eocene eruptions, upper crustal dike intrusion, and gold mineralization approximately coincided temporally with deep intrusion of Eocene sills of granite and quartz diorite and shallower intrusion of the Harrison Pass pluton into the core-complex rocks. Stacked Mesozoic nappes of metamorphosed Paleozoic and Precambrian rocks in the core complex lay at least 13 to 20 km deep in Eocene time, on the basis of geobarometry studies. In the northern part of the complex, the presently exposed rocks had been even deeper in the late Mesozoic, to >30 km depths, before losing part of their cover by Eocene time. Nappes in the core plunge northward beneath the originally thicker Mesozoic tectonic cover in the north part of the core complex. Mesozoic nappes and tectonic wedging likely occupied the thickened midlevel crustal section between the deep crustal core-complex intrusions and nappes and the overlying upper crust. These structures, as well as the subsequent large

  9. Shallow seismic reflection profiling over a Mylonitic Shear Zone, Ruby Mountains-East Humboldt Range Metamorphic Core Complex, NE Nevada

    Science.gov (United States)

    Hawman, Robert B.; Ahmed, Hishameldin O.

    Seismic reflection profiling carried out with a sledgehammer source has imaged Tertiary extensional structures over a depth range of 45-500 m within lower plate rocks of the Ruby Mountains-East Humboldt Range metamorphic core complex. The 400-m CMP profile straddles an exposed contact between tectonic slices of dolomitic marble and metaquartzite emplaced by low-angle ductile-brittle normal faulting. Subhorizontal reflections from layering within the tectonic slices give way at 160 ms (160-220 m depth) to reflections that dip 15-45° to the east, in contrast with dips indicated in a poorly imaged segment of a coincident regional seismic line but in agreement with dips of foliation mapped for nearby up-plunge exposures of a late Proterozoic - early Cambrian sequence of metaquartzites, marbles, schists, and granitic rocks that forms the bulk of the underlying shear zone. Differences with the regional profile are attributed to the higher frequencies (30-100 Hz) generated by the smaller hammer source and the enhanced lateral resolution provided by the straighter profile and much smaller shot-receiver offsets (46-157 m) contributing to the stack for each CMP. The results suggest that the near-surface, east-dipping component of the anastomozing shear zone extends at least 2 km farther east than previously interpreted. Rough estimates of interval velocities (1500-4500 m/s) inferred from stacking velocities are consistent with velocities of mylonitic rocks measured perpendicular to foliation at low confining pressures when the effects of macroscopic fractures and joints are taken into account. Peaks in amplitude spectra of stacked traces suggest long-wavelength components of layering resolved at scales from 5-8 m (depth: 50 m) to 15-25 m (depth: 500 m).

  10. Continental rifting and metamorphic core complex formation ahead of the Woodlark spreading ridge, D'Entrecasteaux Islands, Papua New Guinea

    Science.gov (United States)

    Little, Timothy A.; Baldwin, S. L.; Fitzgerald, P. G.; Monteleone, B.

    2007-02-01

    We evaluate the role of a metamorphic core complex (MCC) on Normanby Island in the Woodlark rift. Located 1 km thickness of blueschist-derived mylonites formed in a midcrustal shear zone during the Pliocene at ˜400-500°C. This top-to-the-north zone appears to have reactivated the gently dipping base of the Papuan ophiolite (Papuan Ultramafic Body, PUB), and its continued activity appears to control the north dipping asymmetry of active half grabens to the north of the MCC and rapid subsidence of the Woodlark Rise. Mylonites in the MCC's lower plate have been exhumed along a detachment as a result of >50 km of slip at rates of >12 mm/yr. The inactive, back-tilted detachment preserves fault surface megamullions and mylonitic lineations parallel to the Plio-Pleistocene plate motion. A second SE vergent detachment has been established on the opposite flank of this rolling-hinge style MCC, probably since 0.8) at depth, and provide a sufficient mechanism for activating low-angle normal faults in the rift. MCC inception was not localized to the tip of the Woodlark MOR. Instead, extreme crustal thinning near the MCC preconditioned later continental breakup. The lower crust appears to be weak, thickening beneath unloaded footwalls to uplift MCCs above sea level, and flowing laterally to even out regional crustal thickness contrasts on a 1-6 m.y. timescale. Deep-seated transforms separate rheologically distinct domains in which extension has been localized along the weak PUB to cause MCC formation, vs. those in which slip is distributed across an imbricate zone of more uniform strength normal faults. The Trobriand fault connects in the eastern Woodlark rift to the Owen Stanley fault in the Papuan Ranges, which is probably moving at nearly the full plate velocity.

  11. Formation of metamorphic core complexes in non-over-thickened continental crust: A case study of Liaodong Peninsula (East Asia)

    Science.gov (United States)

    Wang, Kun; Burov, Evgueni; Gumiaux, Charles; Chen, Yan; Lu, Gang; Mezri, Leila; Zhao, Liang

    2015-12-01

    Pre-thickened hot orogenic crust is often considered a necessary condition for the formation of continental metamorphic core complexes (MCCs). However, the discovery of MCCs in the Liaodong Peninsula, where the crust has a normal thickness (~ 35 km), challenges the universality of this scenario. Therefore, we implement a series of 2-D numerical thermo-mechanical modeling experiments in which we investigate the conditions of MCC formation in normal crusts, as well as the relationships between the underlying mechanisms and the syn-rift basin evolution. In these experiments, we explore the impact of the lithostratigraphic and thermo-rheological structure of the crust. We also examine the lithosphere thickness, strain softening, extension rate, and surface erosion/ sedimentation processes. The experiments demonstrate that high thermal gradients and crustal heterogeneities result only in a symmetric spreading dome, which is geometrically incompatible with the observations of the MCCs in the Liaodong Peninsula. According to our further findings, the strain softening should play a key role in the development of asymmetric strain localization and domal topography uplift, while synchronous surface erosion controls the polarity of the syn-rift basin. The synthetic model data are compatible with the geological observations and cooling history based on the thermo-chronology for the eastern part of the East Asia during the late Mesozoic to the early Cenozoic. The model-predicted P-T-t paths are essentially different from those inferred for the other known MCCs, confirming the exceptional character of the MCC formation in the wide rift system of the East Asia.

  12. Tectonic stratigraphy near a metamorphic core complex: Lessons from the Castaneda-signal area of west-central Arizona

    Energy Technology Data Exchange (ETDEWEB)

    Lucchitta, I. (Geological Survey, Flagstaff, AZ (United States)); Suneson, N.H. (Oklahoma Geological Survey, Norman, OK (United States))

    1993-04-01

    A sequence of latest Oligocene through Quaternary sedimentary and volcanic rocks, when analyzed tectonically and combined with lithologically distinctive source terranes, clarifies the character and timing of Neogene extension just north of the Buckskin-Rawhide metamorphic core complex (BRMCC) in west-central Arizona. The oldest strata (basal arkose of Lucchitta and Suneson) reflect regional stability and a southwesterly paleoslope. In latest Oligocene time, this drainage was ponded by an upwarp (now exposed as the BRMCC) rising to the southwest. The resulting lake beds contain a thin 26.6 MA airfall tuff that marks the beginning of volcanic activity in the region. A widespread breccia records the progressive unroofing of the still-rising CC. Mantle-driven crustal heating probably caused the upwarp and allowed the eruption of voluminous mantle-derived basalt and basaltic andesite about 19 MA (early basalts, Artillery Basalt). The overlying syntectonic conglomerate (arkose of Keenan Camp) was deposited during a period of extreme extension, low-angle detachment faulting, and block rotation, typical of highly extended terranes. The conglomerate is interlayered with widespread silicic volcanic rocks (15--10 MA) derived from the lower crust and large gravity-glide sheets lithologically identical to the breccia and similarly derived from the CC to the south. Unconformably overlying the conglomerate are locally derived fanglomerate and 13--8.5 MA (mesa-forming) basalt that accumulated in present-day basins of classic basin-range type. Untilted and nearly unfaulted 7.7--5.4 MA mantle-derived megacryst-bearing basalt marks the cessation of tectonic activity.

  13. Characterization of the low-temperature triplet state of chlorophyll in photosystem II core complexes: Application of phosphorescence measurements and Fourier transform infrared spectroscopy.

    Science.gov (United States)

    Zabelin, Alexey A; Neverov, Konstantin V; Krasnovsky, Alexander A; Shkuropatova, Valentina A; Shuvalov, Vladimir A; Shkuropatov, Anatoly Ya

    2016-06-01

    Phosphorescence measurements at 77 K and light-induced FTIR difference spectroscopy at 95 K were applied to study of the triplet state of chlorophyll a ((3)Chl) in photosystem II (PSII) core complexes isolated from spinach. Using both methods, (3)Chl was observed in the core preparations with doubly reduced primary quinone acceptor QA. The spectral parameters of Chl phosphorescence resemble those in the isolated PSII reaction centers (RCs). The main spectral maximum and the lifetime of the phosphorescence corresponded to 955±1 nm and of 1.65±0.05 ms respectively; in the excitation spectrum, the absorption maxima of all core complex pigments (Chl, pheophytin a (Pheo), and β-carotene) were observed. The differential signal at 1667(-)/1628(+)cm(-1) reflecting a downshift of the stretching frequency of the 13(1)-keto C=O group of Chl was found to dominate in the triplet-minus-singlet FTIR difference spectrum of core complexes. Based on FTIR results and literature data, it is proposed that (3)Chl is mostly localized on the accessory chlorophyll that is in triplet equilibrium with P680. Analysis of the data suggests that the Chl triplet state responsible for the phosphorescence and the FTIR difference spectrum is mainly generated due to charge recombination in the reaction center radical pair P680(+)PheoD1(-), and the energy and temporal parameters of this triplet state as well as the molecular environment and interactions of the triplet-bearing Chl molecule are similar in the PSII core complexes and isolated PSII RCs.

  14. Results of a Second-generation Constrained Condylar Prosthesis in Complex Primary and Revision Total Knee Arthroplasty: A Mean 5.5-Year Follow-up

    Directory of Open Access Journals (Sweden)

    Chen-Yi Ye

    2016-01-01

    Conclusions: Second-generation modular CCK prostheses are a safe and practical treatment for both primary and revision knees that cannot be balanced. However, further studies focusing on different types of constrained prostheses are required to validate these results.

  15. Curcumin as the OO bidentate ligand in "2 + 1" complexes with the [M(CO)3]+ (M = Re, 99mTc) tricarbonyl core for radiodiagnostic applications.

    Science.gov (United States)

    Sagnou, Marina; Benaki, Dimitra; Triantis, Charalampos; Tsotakos, Theodoros; Psycharis, Vassilis; Raptopoulou, Catherine P; Pirmettis, Ioannis; Papadopoulos, Minas; Pelecanou, Maria

    2011-02-21

    The synthesis and characterization of "2 + 1" complexes of the [M(CO)(3)](+) (M = Re, (99m)Tc) core with the β-diketones acetylacetone (complexes 2, 8) and curcumin (complexes 5, 10 and 6, 11) as bidentate OO ligands, and imidazole or isocyanocyclohexane as monodentate ligands is reported. The complexes were synthesized by reacting the [NEt(4)](2)[Re(CO)(3)Br(3)] precursor with the β-diketone to generate the intermediate aqua complex fac-Re(CO)(3)(OO)(H(2)O) that was isolated and characterized, followed by replacement of the labile water by the monodentate ligand. All complexes were characterized by mass spectrometry, NMR and IR spectroscopies, and elemental analysis. In the case of complex 2, bearing imidazole as the monodentate ligand, X-ray analysis was possible. The chemistry was successfully transferred at (99m)Tc tracer level. The curcumin complexes 5 and 6, as well as their intermediate aqua complex 4, that bear potential for radiopharmaceutical applications due to the wide spectrum of pharmacological activity of curcumin, were successfully tested for selective staining of β-amyloid plaques of Alzheimer's disease. The fact that the complexes maintain the affinity of the mother compound curcumin for β-amyloid plaques prompts for further exploration of their chemistry and biological properties as radioimaging probes.

  16. Simulating Valence-to-Core X-ray Emission Spectroscopy of Transition Metal Complexes with Time-Dependent Density Functional Theory

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yu; Mukamel, Shaul; Khalil, Munira; Govind, Niranjan

    2015-11-09

    Valence-to-core (VtC) X-ray emission spectroscopy (XES) has emerged as a power- ful technique for the structural characterization of complex organometallic compounds in realistic environments. Since the spectrum represents electronic transitions from the ligand molecular orbitals to the core holes of the metal centers, the approach is more chemically sensitive to the metal-ligand bonding character compared with con- ventional X-ray absorption techniques. In this paper we study how linear-response time-dependent density functional theory (LR-TDDFT) can be harnessed to simulate K-edge VtC X-ray emission spectra reliably. LR-TDDFT allows one to go beyond the single-particle picture that has been extensively used to simulate VtC-XES. We con- sider seven low- and high-spin model complexes involving chromium, manganese and iron transition metal centers. Our results are in good agreement with experiment.

  17. Experimental and geologic evaluation of monazite (U-Th)/He thermochronometry: Catnip Sill, Catalina Core Complex, Tucson, AZ

    Science.gov (United States)

    Peterman, E. M.; Hourigan, J. K.; Grove, M.

    2014-10-01

    Monazite is a petrologically important and analytically promising target for (U-Th)/He thermochronology. Previous studies have reported highly variable He diffusion results from monazite from a single sample and demonstrated that composition can significantly affect He diffusion parameters. In this study, we performed incremental heating of single monazite grains to experimentally determine the 4He diffusion properties of reference monazite ‘554’ that occurs within a peraluminous two-mica granite from the Catnip Sill within the Catalina Core complex, Arizona. Assuming that the grain size defines the diffusion geometry, the six experiments yielded Ea values of 212 to 238±5 kJ mol (1σ) and Do values of 15.7 to 103 cm s with one value of 784 cm s. Monazite (U-Th)/He data from five grains yielded closure temperatures of 291 to 262 °C (± c. 15 °C) and ages of 23.8-20.3 (±∼1.2;2σ) Ma; the weighted mean age is 21.8±0.73 (MSWD=1.83, n=5) and the weighted mean closure temperature is 282±6 °C (MSWD=0.96, n=5;1σ). We tested the accuracy of these results by comparing our monazite thermochronology data with monazite Th/Pb depth profiling results, the 40Ar/39Ar thermal history for the Catnip Sill constrained using coexisting muscovite, biotite, and K-feldspar, and published regional zircon and apatite fission track results. The monazite Th/Pb data indicate emplacement of the Catnip Sill at ∼45 Ma. The 40Ar/39Ar muscovite and biotite data indicate cooling from 460 to 350 °C from 27 to 26 Ma. K-feldspar MDD modeling suggests cooling from 360 to 240 °C from 26 to 24 Ma. Zircon fission track data indicate cooling through 250 °C between 29 and 20 Ma. Additional cooling through 110 °C is recorded by apatite fission track ages of 19-16 Ma. Because the monazite thermochronology results are reproducible and consistent with the thermal history constrained by the other chronometers, our results 1) confirm the accuracy of the 4He diffusion kinetics from monazite

  18. THE GEOCHEMISTRY AND AGES OF ROCKS IN THE FOOTWALL OF THE BUTULIYN-NUR AND ZAGAN METAMORPHIC CORE COMPLEXES (NORTH MONGOLIA – WESTERN TRANSBAIKALIA)

    OpenAIRE

    T. V. Donskaya; A.M. MAZUKABZOV

    2015-01-01

    This article reviews data on ages of rocks in the footwall of the Butuliyn-Nur and Zagan metamorphic core complexes (MCC) and provides new data on the geochemistry of the rock complexes. It is noted that the oldest rocks are mylonitized gneisses on rhyolites (554 Ma) in the footwall of the Butuliyn-Nur MCC. The Late Permian – Triassic (249–211 Ma) igneous rocks are ubiquitous in the footwall of the Butuliyn-Nur and Zagan MCC. The youngest rocks in the studied MCC are the Jurassic granitoids (...

  19. Magma mixing in the Kalaqin core complex, northern North China Craton: Linking deep lithospheric destruction and shallow extension

    Science.gov (United States)

    Fu, Lebing; Wei, Junhao; Tan, Jun; Santosh, M.; Zhang, Daohan; Chen, Jiajie; Li, Yanjun; Zhao, Shaoqing; Peng, Lina

    2016-09-01

    The widespread Mesozoic magmatism in the North China Craton (NCC) has received considerable attention as a trigger for large scale lithospheric destruction. Here we investigate the Early Cretaceous Jiguanzi adamellite from the northern part of the NCC which is contemporaneous with shallow extensional deformation and deep lithospheric destruction. This intrusion emplaced at ca. 133 Ma is located in the foot wall of the Kalaqin metamorphic core complex (MCC), and occurs as a synextensional ring complex with numerous magmatic equigranular (Group 1) and porphyritic (Group 2) enclaves. Hornblende and plagioclase from the host adamellite and xenocrysts of Group 2 enclaves show distinct inverse zoning with Mg- and Ca-rich mantle. The Group 2 enclaves are characterized by plagioclase xenocrysts hosting hornblende, biotite and apatite inclusions, quartz ocelli with fine-grained rim enriched in biotite and hornblende, and poikilitic biotite surrounded by hornblende. Geochemically, the host intrusion is calc-alkaline to alkaline and metaluminous with variable contents of SiO2 (60.70-72.20 wt.%), Al2O3 (14.19-17.22 wt.%), Na2O + K2O (6.16-9.42 wt.%), and Mg# values (28.0-47.7), whereas the Group 2 enclaves exhibit low SiO2 (54.05-55.55 wt.%), high Fe2O3 (8.18-8.64 wt.%) and TiO2 (2.08-2.28 wt.%), and moderate Mg# (44.0-44.1). Both the host intrusion and Group 2 enclaves are enriched in large-ion lithophile and light rare earth elements, and depleted in high field strength elements and heavy rare earth elements except that the latter has lower Ba and high Nb, Ta and Ti contents. The major and trace element contents of the Group 1 enclaves are broadly similar to those of the host intrusion. Analyses of Sr-Nd-Hf isotopes in the host intrusion, and in Group 1 and Group 2 enclaves show (87Sr/86Sr)ihost = 0.70600-0.70618, εNd(t)host = - 8.2 to - 9.6, T2DM(Nd)host = 1592-1706 Ma, εHf(t)host = - 9.2 to - 12.0, (87Sr/86Sr)iGroup 1 = 0.70590-0.70635, εNd(t)Group 1 = - 9.6 to - 10

  20. Admissible and Restrained Revision

    CERN Document Server

    Booth, R; 10.1613/jair.1874

    2011-01-01

    As partial justification of their framework for iterated belief revision Darwiche and Pearl convincingly argued against Boutiliers natural revision and provided a prototypical revision operator that fits into their scheme. We show that the Darwiche-Pearl arguments lead naturally to the acceptance of a smaller class of operators which we refer to as admissible. Admissible revision ensures that the penultimate input is not ignored completely, thereby eliminating natural revision, but includes the Darwiche-Pearl operator, Nayaks lexicographic revision operator, and a newly introduced operator called restrained revision. We demonstrate that restrained revision is the most conservative of admissible revision operators, effecting as few changes as possible, while lexicographic revision is the least conservative, and point out that restrained revision can also be viewed as a composite operator, consisting of natural revision preceded by an application of a "backwards revision" operator previously studied by Papini. ...

  1. ACL Revision

    Science.gov (United States)

    Costa-Paz, Matias; Dubois, Julieta Puig; Zicaro, Juan Pablo; Rasumoff, Alejandro; Yacuzzi, Carlos

    2017-01-01

    Objectives: The purpose of this study was to evaluate a series of patients one year after an ACL revision with clinical evaluation and MRI, to consider their condition before returning to sports activities. Methods: A descriptive, prospective and longitudinal study was performed. A series of patients who underwent an ACL revision between March 2014 and March 2015 were evaluated after one year post surgery. They were evaluated using the Lysholm score, IKDC, Tegner, artrometry and MRI (3.0 t). A signal pattern and osteointegration was determined in the MRI. Graft signal intensity of the ACL graft using the signal/noise quotient value (SNQ) was also determined to evaluate the ligamentatization process state. Results: A total of 18 male patients were evaluated with a mean age of 31 years old.Average scores were: Lysholm 88 points, IKDC 80 points, Pre-surgical Tegner 9 points and postoperative 4 points. Artrhometry (KT1000) at 20 newtons showed a side to side difference of less than 3 mm in 88%. Only 44% of patients returned to their previous sport activity one year after revision.The MRI showed a heterogeneous signal in neoligaments in 34% of patients. SNQ showed graft integration in only 28%. Synovial fluid was found in bone-graft interphase in 44% of tunnels, inferring partial osteointegration. The heterogeneous signal was present in 50% of patients who did not return to the previous sport level activity. (Fisher statistics: p = 0.043) There were no meaningful differences in patients with auto or allografts. Conclusion: Although the clinical evaluation was satisfactory, only 44% of patients returned to the previous level of sport activity one year after the ACL surgery. The ligamentatization process was found in 28% of knees evaluated with MRI one year later. Partial osteointegration is inferred in 44%. Results showed a meaningful relation between the signal of neoligaments in the MRI and the return to sport activity in said series of patients. MRI is a useful tool

  2. Crystal Structure of a Complex of Surfactant Protein D (SP-D) and Haemophilus influenzae Lipopolysaccharide Reveals Shielding of Core Structures in SP-D-Resistant Strains.

    Science.gov (United States)

    Clark, Howard W; Mackay, Rose-Marie; Deadman, Mary E; Hood, Derek W; Madsen, Jens; Moxon, E Richard; Townsend, J Paul; Reid, Kenneth B M; Ahmed, Abdul; Shaw, Amy J; Greenhough, Trevor J; Shrive, Annette K

    2016-05-01

    The carbohydrate recognition domains (CRDs) of lung collectin surfactant protein D (SP-D) recognize sugar patterns on the surface of lung pathogens and promote phagocytosis. Using Haemophilus influenzae Eagan strains expressing well-characterized lipopolysaccharide (LPS) surface structures of various levels of complexity, we show that bacterial recognition and binding by SP-D is inversely related to LPS chain extent and complexity. The crystal structure of a biologically active recombinant trimeric SP-D CRD complexed with a delipidated Eagan 4A LPS suggests that efficient LPS recognition by SP-D requires multiple binding interactions utilizing the three major ligand-binding determinants in the SP-D binding pocket, with Ca-dependent binding of inner-core heptose accompanied by interaction of anhydro-Kdo (4,7-anhydro-3-deoxy-d-manno-oct-2-ulosonic acid) with Arg343 and Asp325. Combined with enzyme-linked immunosorbent assays (ELISAs) and fluorescence-activated cell sorter (FACS) binding analyses, our results show that extended LPS structures previously thought to be targets for collectins are important in shielding the more vulnerable sites in the LPS core, revealing a mechanism by which pathogens with complex LPS extensions efficiently evade a first-line mucosal innate immune defense. The structure also reveals for the first time the dominant form of anhydro-Kdo.

  3. Microparticles obtained by complex coacervation: influence of the type of reticulation and the drying process on the release of the core material

    Directory of Open Access Journals (Sweden)

    Izabela Dutra Alvim

    2010-12-01

    Full Text Available Microparticles obtained by complex coacervation were crosslinked with glutaraldehyde or with transglutaminase and dried using freeze drying or spray drying. Moist samples presented Encapsulation Efficiency (%EE higher than 96%. The mean diameters ranged from 43.7 ± 3.4 to 96.4 ± 10.3 µm for moist samples, from 38.1 ± 5.36 to 65.2 ± 16.1 µm for dried samples, and from 62.5 ± 7.5 to 106.9 ± 26.1 µm for rehydrated microparticles. The integrity of the particles without crosslinking was maintained when freeze drying was used. After spray drying, only crosslinked samples were able to maintain the wall integrity. Microparticles had a round shape and in the case of dried samples rugged walls apparently without cracks were observed. Core distribution inside the particles was multinuclear and homogeneous and core release was evaluated using anhydrous ethanol. Moist particles crosslinked with glutaraldehyde at the concentration of 1.0 mM.g-1 protein (ptn, were more efficient with respect to the core retention compared to 0.1 mM.g-1 ptn or those crosslinked with transglutaminase (10 U.g-1 ptn. The drying processes had a strong influence on the core release profile reducing the amount released to all dry samples

  4. Hetero-metallic trigonal cage-shaped dimeric Ni3K core complex of L-proline ligand: Synthesis, structural, electrochemical and DNA binding and cleavage activities

    Indian Academy of Sciences (India)

    S Nagasubramanian; A Jayamani; V Thamilarasan; G Aravindan; V Ganesan; N Sengottuvelan

    2014-05-01

    Hetero-metallic trigonal cage-shaped dimeric Ni3K core complex of L-proline ligand has been synthesized and characterized. Single crystal X-ray diffraction analysis showed that the hetero-metallic Ni(II)-K(I) complex has a dimeric structure with nine coordinated potassium atoms and six coordinated nickel atoms. The cyclic voltammograms of the complex exhibited two successive quasireversible reduction waves at ($E^{1}_{pc} = −1.02$ V and $E^{2}_{pc} = −1.33$ V) and two successive irreversible oxidation waves ($E^{1}_{pa} = 0.95$ V and $E^{2}_{pa} = 1.45$ V) versus Ag/AgCl in DMF solution. Interaction of the complex with calf-thymus DNA (CT DNA) has been studied using spectroscopic techniques. The complex is an avid DNA binder with a binding constant of 3.6 × 108 M-1. The complex showed efficient oxidative cleavage of supercoiled pBR322 DNA in the presence of the reducing agent hydrogen peroxide involving hydroxyl radical (°OH) species. As evidenced from the control experiment, DNA cleavage in the presence of °OH radical was inhibited by quenchers, viz. DMSO and KI. The complex showed in vitro antimicrobial activity against four bacteria and two fungi and the activity is greater than that of the free ligand.

  5. Complex sclerosing lesions and radial sclerosing lesions on core needle biopsy: Low risk of carcinoma on excision in cases with clinical and imaging concordance.

    Science.gov (United States)

    Nakhlis, Faina; Lester, Susan; Denison, Christine; Wong, Stephanie M; Mongiu, Anne; Golshan, Mehra

    2017-07-07

    Complex or radial sclerosing lesions (CSL/RSL) are uncommon diagnoses on core needle biopsy with a reported upgrade rate ranging between 0% and 23%. As a result, their management remains controversial. In this study, we sought to determine the rate of malignancy on excision for patients with pure CSL/RSL on core biopsy, and to evaluate future breast cancer risk when CSL/RSL is managed without excision. We retrospectively reviewed 118 cases of CSL/RSL diagnosed on image-guided breast biopsies between 2005 and 2014 at our institution. Of 98 analyzed patients, 34 (35%) underwent excision and 64 (65%) were observed. Demographic and clinical variables between excision and observation groups were compared. In excised specimens, factors associated with upgrade to malignancy were evaluated. The median age at diagnosis was 49 years (range, 27-88 years). In the excision group, 3/34 cases were associated with malignancy, an overall upgrade rate of 9%. All malignant cases had core needle biopsies interpreted as discordant and were BIRADS 4B or more on imaging. In the observation group, at a median follow-up of 2.2 years, 3/64 (5%) patients developed ipsilateral cancers, all of which were distant from the index CSL/RSL. In our series, we report a 9% malignancy rate on excision of BIRADS >4C lesions characterized as CSL/RSL on core biopsy. In patients with concordant biopsies and BIRADS 4A or lower lesions who underwent observation, we found a low rate of subsequent ipsilateral cancers. Further studies are needed to confirm that for CSL/RSL in concordant core biopsies and BIRADS 4A or lower, nonpalpable lesions, observation may be a reasonable alternative to excision. © 2017 Wiley Periodicals, Inc.

  6. Synthesis, crystal structure and interaction of L-valine Schiff base divanadium(V) complex containing a V2O3 core with DNA and BSA

    Science.gov (United States)

    Guo, Qiong; Li, Lianzhi; Dong, Jianfang; Liu, Hongyan; Xu, Tao; Li, Jinghong

    2013-04-01

    A divanadium(V) complex, [V2O3(o-van-val)2] (o-van-val = Schiff base derived from o-vanillin and L-valine), has been synthesized and structurally characterized. The crystal structure shows that both of the vanadium centers in the complex have a distorted octahedral coordination environment composed of tridentate Schiff base ligand. A V2O3 core in molecular structure adopts intermediate between cis and trans configuration with the O1dbnd V1⋯V1Adbnd O1A torsion angle 115.22 (28)° and the V1⋯V1A distance 3.455 Å. The binding properties of the complex with calf thymus DNA (CT-DNA) have been investigated by UV-vis absorption, fluorescence, CD spectra and viscosity measurement. The results indicate that the complex binds to CT-DNA in non-classical intercalative mode. Meanwhile, the interaction of the complex with bovine serum albumin (BSA) has been studied by UV-vis absorption, fluorescence and CD spectra. Results indicated that the complex can markedly quench the intrinsic fluorescence of BSA via a static quenching process, and cause its conformational change. The calculated apparent binding constant Kb was 1.05 × 106 M-1 and the binding site number n was 1.18.

  7. Synthesis, crystal structure and interaction of L-valine Schiff base divanadium(V) complex containing a V2O3 core with DNA and BSA.

    Science.gov (United States)

    Guo, Qiong; Li, Lianzhi; Dong, Jianfang; Liu, Hongyan; Xu, Tao; Li, Jinghong

    2013-04-01

    A divanadium(V) complex, [V2O3(o-van-val)2] (o-van-val=Schiff base derived from o-vanillin and L-valine), has been synthesized and structurally characterized. The crystal structure shows that both of the vanadium centers in the complex have a distorted octahedral coordination environment composed of tridentate Schiff base ligand. A V2O3 core in molecular structure adopts intermediate between cis and trans configuration with the O1V1⋯V1AO1A torsion angle 115.22 (28)° and the V1⋯V1A distance 3.455Å. The binding properties of the complex with calf thymus DNA (CT-DNA) have been investigated by UV-vis absorption, fluorescence, CD spectra and viscosity measurement. The results indicate that the complex binds to CT-DNA in non-classical intercalative mode. Meanwhile, the interaction of the complex with bovine serum albumin (BSA) has been studied by UV-vis absorption, fluorescence and CD spectra. Results indicated that the complex can markedly quench the intrinsic fluorescence of BSA via a static quenching process, and cause its conformational change. The calculated apparent binding constant Kb was 1.05×10(6)M(-1) and the binding site number n was 1.18.

  8. Synthesis, molecular docking and evaluation of antifungal activity of Ni(II), Co(II) and Cu(II) complexes of porphyrin core macromolecular ligand.

    Science.gov (United States)

    Singh, Urvashi; Malla, Ali Mohammad; Bhat, Imtiyaz Ahmad; Ahmad, Ajaz; Bukhari, Mohd Nadeem; Bhat, Sneha; Anayutullah, Syed; Hashmi, Athar Adil

    2016-04-01

    Porphyrin core dendrimeric ligand (L) was synthesized by Rothemund synthetic route in which p-hydroxy benzaldehyde and pyrrole were fused together. The prepared ligand was complexed with Ni(II), Cu(II) and Co(II) ions, separately. Both the ligand and its complexes were characterized by elemental analysis and spectroscopic studies (FT-IR, UV-Vis, (1)HNMR). Square planar geometries were proposed for Cu(II), Ni(II) and Co(II) ions in cobalt, Nickel and copper complexes, respectively on the basis of UV-Vis spectroscopic data. The ligand and its complex were screened on Candida albicans (ATCC 10231), Aspergillus fumigatus (ATCC 1022), Trichophyton mentagrophytes (ATCC 9533) and Pencillium marneffei by determining MICs and inhibition zones. The activity of the ligand and its complexes was found to be in the order: CuL ˃ CoL ≈ NiL ˃ L. Detection of DNA damage at the level of the individual eukaryotic cell was observed by commet assay. Molecular docking technique was used to understand the ligand-DNA interactions. From docking experiment, we conclude that copper complex interacts more strongly than rest two.

  9. Comparison between results of detailed tectonic studies on borehole core vs microresistivity images of borehole wall from gas-bearing shale complexes, Baltic Basin, Poland.

    Science.gov (United States)

    Bobek, Kinga; Jarosiński, Marek; Pachytel, Radomir

    2017-04-01

    , cemented with calcite, were clearly visible in scanner image. We have also observed significantly lower density of veins in core than in the XRMI that occurs systematically in one formation enriched with carbonate and dolomite. In this case, veins are not fractured in core and obliterated for bare eye by dolomitization, but are still contrastive in respect of electric resistance. Calculated density of bedding planes per 1 meter reveals systematically higher density of fractures observed on core than in the XRMI (depicted automatically by interpretation program). This difference may come from additional fracking due to relaxation of borehole core while recovery. Comparison of vertical joint fractures density with thickness of mechanical beds shows either lack of significant trends or a negative correlation (greater density of bedding fractures correspond to lower density of joints). This result, obtained for shale complexes contradict that derived for sandstone or limestone. Boundary between CLUs are visible on both: joint and bedding fracture density profiles. Considering small-scale faults and slickensides we have obtained good agreement between results of core and scanner interpretation. This study in the frame of ShaleMech Project funded by Polish Committee for Scientific Research is in progress and the results are preliminary.

  10. Modeling of the catalytic core of Arabidopsis thaliana Dicer-like 4 protein and its complex with double-stranded RNA.

    Science.gov (United States)

    Mickiewicz, Agnieszka; Sarzyńska, Joanna; Miłostan, Maciej; Kurzyńska-Kokorniak, Anna; Rybarczyk, Agnieszka; Łukasiak, Piotr; Kuliński, Tadeusz; Figlerowicz, Marek; Błażewicz, Jacek

    2017-02-01

    Plant Dicer-like proteins (DCLs) belong to the Ribonuclease III (RNase III) enzyme family. They are involved in the regulation of gene expression and antiviral defense through RNA interference pathways. A model plant, Arabidopsis thaliana encodes four DCL proteins (AtDCL1-4) that produce different classes of small regulatory RNAs. Our studies focus on AtDCL4 that processes double-stranded RNAs (dsRNAs) into 21 nucleotide trans-acting small interfering RNAs. So far, little is known about the structures of plant DCLs and the complexes they form with dsRNA. In this work, we present models of the catalytic core of AtDCL4 and AtDCL4-dsRNA complex constructed by computational methods. We built a homology model of the catalytic core of AtDCL4 comprising Platform, PAZ, Connector helix and two RNase III domains. To assemble the AtDCL4-dsRNA complex two modeling approaches were used. In the first method, to establish conformations that allow building a consistent model of the complex, we used Normal Mode Analysis for both dsRNA and AtDCL4. The second strategy involved template-based approach for positioning of the PAZ domain and manual arrangement of the Connector helix. Our results suggest that the spatial orientation of the Connector helix, Platform and PAZ relative to the RNase III domains is crucial for measuring dsRNA of defined length. The modeled complexes provide information about interactions that may contribute to the relative orientations of these domains and to dsRNA binding. All these information can be helpful for understanding the mechanism of AtDCL4-mediated dsRNA recognition and binding, to produce small RNA of specific size.

  11. Dense SDM (12-core × 3-mode) transmission over 527 km with 33.2-ns mode-dispersion employing low-complexity parallel MIMO frequency-domain equalization

    DEFF Research Database (Denmark)

    Shibahara, K.; Mizuno, T.; Takara, H.;

    We demonstrate 12-core × 3-mode dense SDM transmission over 527 km graded-index multi-core few-mode fiber without mode-dispersion management. Employing low baud rate multi-carrier signal and frequency-domain equalization enables 33.2-ns DMD compensation with low computational complexity. © 2015 OSA...

  12. Three-dimensional structure of the ligand-binding core of GluR2 in complex with the agonist (S)-ATPA: implications for receptor subunit selectivity.

    Science.gov (United States)

    Lunn, Marie-Louise; Hogner, Anders; Stensbøl, Tine B; Gouaux, Eric; Egebjerg, Jan; Kastrup, Jette S

    2003-02-27

    Two X-ray structures of the GluR2 ligand-binding core in complex with (S)-2-amino-3-(5-tert-butyl-3-hydroxy-4-isoxazolyl)propionic acid ((S)-ATPA) have been determined with and without Zn(2+) ions. (S)-ATPA induces a domain closure of ca. 21 degrees compared to the apo form. The tert-butyl moiety of (S)-ATPA is buried in a partially hydrophobic pocket and forces the ligand into the glutamate-like binding mode. The structures provide new insight into the molecular basis of agonist selectivity between AMPA and kainate receptors.

  13. New cyclic tetranuclear copper(II) complexes containing quadrilateral cores: Synthesis, structure, spectroscopy and their interactions with DNA in aqueous solution

    Science.gov (United States)

    Giri, Gopal C.; Haldar, Shobhraj; Ghosh, Aloke Kumar; Chowdhury, Priyanka; Carrella, Luca; Ghosh, Utpal; Bera, Manindranath

    2017-08-01

    Three new cyclic tetranuclear copper(II) complexes, Tetrakis{3-[(2-pyridylmethyl)-amino]-propionato}(tetrachloro)tetracopper(II)methanolhydrate (1·CH3OH·H2O), Tetrakis{3-[(2-pyridylmethyl)-amino]-propionato}(tetrathiocyanato)tetracopper(II) (2) and Tetrakis{3-[(2-pyridylmethyl)-amino]-propionato}(tetraazido)tetracopper(II) (3) have been synthesized by exploiting the chelating ability and bridging potential of a carboxyamine functionalized tridentate ligand, HL (HL = 3-[(2-Pyridylmethyl)-amino]-propionic acid). Complexes 1, 2 and 3 have been synthesized by carrying out reaction of the ligand HL with stoichiometric amounts of CuCl2·2H2O, CuCl2·2H2O/NH4SCN, and CuCl2·2H2O/NaN3, respectively, in the presence of NMe4OH at ambient temperature. Various analytical techniques have been employed to characterize the complexes, including single crystal X-ray diffraction study of 1. Structures of complexes 2 and 3 have been optimized by DFT calculation at B3LYP/6-311G level. Analysis of X-ray crystal structure reveals that the metallic core of complex 1 contains four distorted square pyramidal Cu(II) ions. The Cu(II) ions in each complex are arranged at the corners of a quadrilateral showing a μ2:η1:η1syn-anti bidentate bridging mode of four carboxylate groups of L- ligands with each bridging between two Cu(II) ions. These complexes represent a new family of 16-MCCuII-4 metallocoronates with repeating -[CuIIsbnd Osbnd Csbnd O]- units. In aqueous solution (pH∼7.5), the interactions of complexes with DNA have been investigated by UV-Vis and fluorescence titration spectroscopy, and viscosity measurements.

  14. Mixed chloride/phosphine complexes of the dirhenium core. 10. Redox reactions of an edge-sharing dirhenium(III) non-metal-metal-bonded complex, Re(2)(mu-Cl)(2)Cl(4)(PMe(3))(4).

    Science.gov (United States)

    Cotton, F A; Dikarev, E V; Petrukhina, M A

    2001-12-17

    Reduction and oxidation reactions of the dirhenium(III) non-metal-metal-bonded edge-sharing complex, Re(2)(mu-Cl)(2)Cl(4)(PMe(3))(4) (1), have been studied. Several new mono- and dinuclear rhenium compounds have been isolated and structurally characterized in the course of this study. Reductions of 1 with 1 and 2 equiv of KC(8) result in an unusual face-sharing complex having an Re(2)(5+) core, Re(2)(mu-Cl)(3)Cl(2)(PMe(3))(4) (2), and a triply bonded Re(II) compound, 1,2,7,8-Re(2)Cl(4)(PMe(3))(4) (3), respectively. Two-electron reduction of 1 in the presence of tetrabutylammonium chloride affords a new triply bonded complex of the Re(2)(4+) core, [Bu(n)()(4)N][1,2,7-Re(2)Cl(5)(PMe(3))(3)] (4). Oxidation of 1 with NOBF(4) yields a Re(IV) mononuclear compound, trans-ReCl(4)(PMe(3))(2) (5). Two isomers of the monomeric Re(III) anion, [ReCl(4)(PMe(3))(2)](-) (6, 7), have been isolated as side products. The crystal structures of compounds 2 and 4-7 have been determined by X-ray crystallography. The Re-Re distance in the face-sharing complex 2 of 2.686(1) A is relatively short. The metal-metal bond length in anion 4 of 2.2354(7) A is consistent with the usual values for the triply bonded Re(2)(4+) core compounds. In addition, a cis arrangement of trimethylphosphine ligands in the starting material 1 is retained upon reduction in the dinuclear products 2-4.

  15. Polyaryl ether dendrimer with a 4-phenylacetyl-5-pyrazolone-based terbium(III) complex as core: synthesis and photophysical properties.

    Science.gov (United States)

    Shen, Li; Shi, Mei; Li, Fuyou; Zhang, Dengqing; Li, Xianghong; Shi, Enxian; Yi, Tao; Du, Yukou; Huang, Chunhui

    2006-08-07

    A series of novel dendritic beta-diketone ligands, 1-phenyl-3-[G-n]-4-phenylacetyl-5-pyrazolone (n = 0-3, G stands for polyaryl ether), were synthesized by introducing Fréchet-type dendritic branches. The corresponding Tb3+-cored dendritic complexes were characterized by X-ray crystallography, elemental analysis, ESI mass spectra, and FT-IR spectra. These dendritic complexes, prepared from aqueous solution, exhibit high stability. Interestingly, the study of photophysical properties shows that the luminescence quantum yields of the dendritic Tb-complexes increase from 0.1 to 2.26% with an increase of the dendritic generation from 0 to 3. Importantly, an "energy-reservoir effect" was observed in the dendritic system using the method based on the resonance energy transfer from these complexes to rhodamine 6G. With the increase of the dendritic generation, the metal-centered luminescence quantum yield was almost the same, and the energy transfer (phi(transfer)) from the ligand to Tb(3+) increased. Further measurements of the triplet state and oxygen quenching of these dendritic complexes verify that this enhancement of the energy transfer (phi(transfer)) is attributed to both an "antenna effect" and a "shell effect".

  16. Short scale variation in presence and structure of complex core-mantle boundary regions beneath northern Mexico

    Science.gov (United States)

    Jasbinsek, J. J.

    2016-12-01

    A set of nine intermediate depth earthquakes with closely spaced epicenters in Central America recorded at a small aperture array in the western United States contain clear core-mantle boundary (CMB) reflections. Cross-correlation of [0.5,2] Hz bandpass filtered seismograms at the 11 station array results in well-constrained stacked PcP and ScP waveforms. Most events contain both PcP and ScP waveforms, providing two distinct areas of core-mantle boundary sampling. In approximately half of the stacked waveforms, additional pre- and/or post-cursory arrivals are observed with both PcP and ScP suggesting the presence of complicated CMB structures. Commonly the extra arrivals have the visual appearance of reverberations. Two primary observations are made: (1) One-dimensional forward modeling indicates that simple one-layer ultra-low velocity zone (ULVZ) models do not accurately reproduce the PcP and ScP waveforms, instead multi-layer ULVZ models provide a better fit to the waveforms, (2) Spatially the pattern of CMB regions requiring extra structure is contiguous, but change to a simple CMB structure over short distance scales. The simple one-dimensional modeling explored here cannot uniquely constrain the three-dimensional CMB structure, but provides insight into potential CMB structure that may be resolvable with higher accuracy and more computationally intensive forward seismogram modeling.

  17. Development and testing of a compact basis set for use in effective core potential calculations on rhodium complexes.

    Science.gov (United States)

    Roscioni, Otello M; Lee, Edmond P F; Dyke, John M

    2012-10-05

    We present a set of effective core potential (ECP) basis sets for rhodium atoms which are of reasonable size for use in electronic structure calculations. In these ECP basis sets, the Los Alamos ECP is used to simulate the effect of the core electrons while an optimized set of Gaussian functions, which includes polarization and diffuse functions, is used to describe the valence electrons. These basis sets were optimized to reproduce the ionization energy and electron affinity of atomic rhodium. They were also tested by computing the electronic ground state geometry and harmonic frequencies of [Rh(CO)(2) μ-Cl](2) , Rh(CO)(2) ClPy, and RhCO (neutral and its positive, and negative ions) as well as the enthalpy of the reaction of [Rh(CO)(2) μ-Cl](2) with pyridine (Py) to give Rh(CO)(2) ClPy, at different levels of theory. Good agreement with experimental values was obtained. Although the number of basis functions used in our ECP basis sets is smaller than those of other ECP basis sets of comparable quality, we show that the newly developed ECP basis sets provide the flexibility and precision required to reproduce a wide range of chemical and physical properties of rhodium compounds. Therefore, we recommend the use of these compact yet accurate ECP basis sets for electronic structure calculations on molecules involving rhodium atoms. Copyright © 2012 Wiley Periodicals, Inc.

  18. Time-resolved spectroscopic analysis of the light-energy harvesting mechanism in carbazole-dendrimers with a blue-phosphorescent Ir-complex core.

    Science.gov (United States)

    Cho, Yang-Jin; Kim, So-Yoen; Son, Mi Rang; Son, Ho-Jin; Cho, Dae Won; Kang, Sang Ook

    2017-08-02

    In order to investigate the light-energy harvesting mechanism, a series of dendrimers with a heteroleptic iridium(iii) complex core, [Ir(dmb)2(pic-Czn)] (Gn: n = 1, 2, and 3), with 2,6-difluoro-3-(4-methylpyridin-2-yl)benzonitrile (dmb) as the cyclometallating ligand and 3-hydroxypicolinate (pic) as the ancillary ligand, connected to carbazole-based dendrons (Czn: n = 2, 4, and 8) was synthesized. The Ir centred complex [Ir(dmb)2(pic-OCH3), G0] shows a blue emission at SVD). The determination of the absorption spectra of the individual species participating in the energy transfer process by SVD analysis can distinguish between different mechanistic models. The analysed rate constants were consistent with the results determined by the emission decays.

  19. Essential role of BAF complex interacting with Pax6 in establishment of a core cross-regulatory neurogenic network

    Science.gov (United States)

    Ninkovic, Jovica; Steiner-Mezzadri, Andrea; Jawerka, Melanie; Akinci, Umut; Masserdotti, Giacomo; Petricca, Stefania; Fischer, Judith; von Holst, Alexander; Beckers, Johanes; Lie, Chichung D.; Petrik, David; Miller, Erik; Tang, Jiong; Wu, Jiang; Lefebvre, Veronique; Demmers, Jeroen; Eisch, Amelia; Metzger, Daniel; Crabtree, Gerald; Irmler, Martin; Poot, Raymond; Götz, Magdalena

    2014-01-01

    The molecular mechanisms of neurogenic fate determination are of particular importance in light of the need to regenerate neurons. Here we define the mechanisms of installing neurogenic fate by the transcription factor Pax6 acting together with the Brg1-containing BAF chromatin remodeling complex. We show that Pax6 physically interacts with Brg1-containing BAF complex and genetic deletion of either Pax6 or Brg1, in the neural stem cells in the adult mouse subependymal zone results in a strikingly similar fate conversion from neuronal progenitors to glia. The Pax6-BAF complex drives neurogenesis by directly activating transcription factors Sox11, Nfib and Pou3f4, which form a cross-regulatory network that maintains neurogenic fate downstream of the Pax6-BAF complex in neuroblasts. Our work identifies a novel concept of stratification in neural fate commitment with a strikingly specific role of the Pax6-BAF complex in initiating a cross-regulatory network essential for maintenance of the neurogenic lineage in the adult brain. PMID:23933087

  20. Ca(2+)-binding reduces conformational flexibility of RC-LH1 core complex from thermophile Thermochromatium tepidum.

    Science.gov (United States)

    Jakob-Grun, Selma; Radeck, Jara; Braun, Paula

    2012-03-01

    The light-harvesting complex, LH1, of thermophile purple bacteria Thermochromatium tepidum consists of an array of α- and β-polypeptides which assemble the photoactive bacteriochlorophyll and closely interact with the membrane-lipids. In this study, we investigated the effect of calcium and manganese ions on the protein structure and thermostability of the reaction centre (RC)-LH1/lipid complex. The binding of Ca(2+), but not Mn(2+) is shown to shift the LH1 Q ( y ) absorption maximum from ~889 to 915 nm and to significantly raise the thermostability of the RC-LH1 complex. The ATR-FTIR spectra indicate that interaction of Ca(2+) as monitored by the carboxylates' vibration of aspartate residues, but not Mn(2+) induces changes in the α-helix packing arrangement. The reduced rate of (1)H/(2)H exchange of proteins' amide protons shows that the accessibility to (2)H(2)O is significantly lowered in Ca(2+)-substituted RC-LH1/lipid complexes. In particular, exchange with the associated lipid molecules, is significantly retarded. These results suggest that the thermostability of the RC-LH1 complex is raised by the distinct interaction with calcium cations which reduces the RC-LH1/lipid dynamics, particularly, at the membrane-water interface.

  1. AcsA-AcsB: The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769.

    Science.gov (United States)

    McManus, John B; Deng, Ying; Nagachar, Nivedita; Kao, Teh-hui; Tien, Ming

    2016-01-01

    The gram-negative bacterium, Gluconacetobacter hansenii, produces cellulose of exceptionally high crystallinity in comparison to the cellulose of higher plants. This bacterial cellulose is synthesized and extruded into the extracellular medium by the cellulose synthase complex (CSC). The catalytic component of this complex is encoded by the gene AcsAB. However, several other genes are known to encode proteins critical to cellulose synthesis and are likely components of the bacterial CSC. We have purified an active heterodimer AcsA-AcsB from G. hansenii ATCC23769 to homogeneity by two different methods. With the purified protein, we have determined how it is post-translationally processed, forming the active heterodimer AcsA-AcsB. Additionally, we have performed steady-state kinetic studies on the AcsA-AcsB complex. Finally through mutagenesis studies, we have explored the roles of the postulated CSC proteins AcsC, AcsD, and CcpAx.

  2. Group IVA irons: New constraints on the crystallization and cooling history of an asteroidal core with a complex history

    Science.gov (United States)

    McCoy, T. J.; Walker, R. J.; Goldstein, J. I.; Yang, J.; McDonough, W. F.; Rumble, D.; Chabot, N. L.; Ash, R. D.; Corrigan, C. M.; Michael, J. R.; Kotula, P. G.

    2011-11-01

    We report analyses of 14 group IVA iron meteorites, and the ungrouped but possibly related, Elephant Moraine (EET) 83230, for siderophile elements by laser ablation ICP-MS and isotope dilution. EET was also analyzed for oxygen isotopic composition and metallographic structure, and Fuzzy Creek, currently the IVA with the highest Ni concentration, was analyzed for metallographic structure. Highly siderophile elements (HSE) Re, Os and Ir concentrations vary by nearly three orders of magnitude over the entire range of IVA irons, while Ru, Pt and Pd vary by less than factors of five. Chondrite normalized abundances of HSE form nested patterns consistent with progressive crystal-liquid fractionation. Attempts to collectively model the HSE abundances resulting from fractional crystallization achieved best results for 3 wt.% S, compared to 0.5 or 9 wt.% S. Consistent with prior studies, concentrations of HSE and other refractory siderophile elements estimated for the bulk IVA core and its parent body are in generally chondritic proportions. Projected abundances of Pd and Au, relative to more refractory HSE, are slightly elevated and modestly differ from L/LL chondrites, which some have linked with group IVA, based on oxygen isotope similarities. Abundance trends for the moderately volatile and siderophile element Ga cannot be adequately modeled for any S concentration, the cause of which remains enigmatic. Further, concentrations of some moderately volatile and siderophile elements indicate marked, progressive depletions in the IVA system. However, if the IVA core began crystallization with ˜3 wt.% S, depletions of more volatile elements cannot be explained as a result of prior volatilization/condensation processes. The initial IVA core had an approximately chondritic Ni/Co ratio, but a fractionated Fe/Ni ratio of ˜10, indicates an Fe-depleted core. This composition is most easily accounted for by assuming that the surrounding silicate shell was enriched in iron

  3. Revealing and tuning the core, structure, properties and function of polymer micelles with lanthanide-coordination complexes

    NARCIS (Netherlands)

    Wang, J.; Groeneveld, A.; Oikonomou, M.E.; Prusova, A.; As, van H.; Lent, van J.W.M.; Velders, A.H.

    2016-01-01

    Controlling self-assembly processes is of great interest in various fields where multifunctional and tunable materials are designed. We here present the versatility of lanthanide-complex-based micelles (Ln-C3Ms) with tunable coordination structures and corresponding functions (e.g. luminescence and

  4. ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.

    Science.gov (United States)

    Edelmann, Franziska T; Niedner, Annika; Niessing, Dierk

    2015-01-01

    Asymmetric ASH1 mRNA transport during mitosis of budding yeast constitutes one of the best-studied examples of mRNA localization. Recently, 2 studies used in vitro motility assays to prove that motile ASH1 mRNA-transport complexes can be reconstituted entirely from recombinant factors. Both studies, however, differed in their conclusions on whether cargo RNA itself is required for particle assembly and thus activation of directional transport. Here we provide direct evidence that stable complexes do assemble in absence of RNA at physiologic conditions and even at ionic strengths above cellular levels. These results directly confirm the previous notion that the ASH1 transport machinery is not activated by the cargo RNA itself, but rather through protein-protein interactions.

  5. Determining the Concentration Dependent Transformations of Ag Nanoparticles in Complex Media: Using SP-ICP-MS and Au@Ag Core-Shell Nanoparticles as Tracers.

    Science.gov (United States)

    Merrifield, Ruth C; Stephan, Chady; Lead, Jamie

    2017-03-01

    The fate, behavior, and impact of engineered nanoparticles (NPs) in toxicological and environmental media are driven by complex processes which are difficult to quantify. A key limitation is the ability to perform measurements at low and environmentally relevant concentrations, since concentration may be a key factor determining fate and effects. Here, we use single particle inductively coupled mass spectroscopy (SP-ICP-MS) to measure directly NP diameter and particle number concentration of suspensions containing gold-silver core-shell (Au@Ag) NPs in EPA moderately hard water (MHW) and MHW containing 2.5 mg L(-1) Suwannee River fulvic acid. The Au core of the Au@Ag NPs acts as an internal standard, and aids in the analysis of the complex Ag transformations. The high sensitivity of SP-ICP-MS, along with the Au@Ag NPs, enabled us to track the NP transformations in the range 0.01 and 50 μg L(-1), without further sample preparation. On the basis of the analysis of both Au and Ag parameters (size, size distribution, and particle number), concentration was shown to be a key factor in NP behavior. At higher concentration, NPs were in an aggregation-dominated regime, while at the lower and environmentally representative concentrations, dissolution of Ag was dominant and aggregation was negligible. In addition, further formation of ionic silver as Ag NPs in the form of AgS or AgCl was shown to occur. Between 1 and 10 μg L(-1), both aggregation and dissolution were important. The results suggest that, under realistic conditions, the role of NP homoaggregation may be minimal. In addition, the complexity of exposure and dose in dose-response relationships is highlighted.

  6. An ‘open’ structure of the RecOR complex supports ssDNA binding within the core of the complex

    Science.gov (United States)

    Radzimanowski, Jens; Dehez, François; Round, Adam; Bidon-Chanal, Axel; McSweeney, Sean; Timmins, Joanna

    2013-01-01

    Efficient DNA repair is critical for cell survival and the maintenance of genome integrity. The homologous recombination pathway is responsible for the repair of DNA double-strand breaks within cells. Initiation of this pathway in bacteria can be carried out by either the RecBCD or the RecFOR proteins. An important regulatory player within the RecFOR pathway is the RecOR complex that facilitates RecA loading onto DNA. Here we report new data regarding the assembly of Deinococcus radiodurans RecOR and its interaction with DNA, providing novel mechanistic insight into the mode of action of RecOR in homologous recombination. We present a higher resolution crystal structure of RecOR in an ‘open’ conformation in which the tetrameric RecR ring flanked by two RecO molecules is accessible for DNA binding. We show using small-angle neutron scattering and mutagenesis studies that DNA binding does indeed occur within the RecR ring. Binding of single-stranded DNA occurs without any major conformational changes of the RecOR complex while structural rearrangements are observed on double-stranded DNA binding. Finally, our molecular dynamics simulations, supported by our biochemical data, provide a detailed picture of the DNA binding motif of RecOR and reveal that single-stranded DNA is sandwiched between the two facing oligonucleotide binding domains of RecO within the RecR ring. PMID:23814185

  7. Complexity

    CERN Document Server

    Gershenson, Carlos

    2011-01-01

    The term complexity derives etymologically from the Latin plexus, which means interwoven. Intuitively, this implies that something complex is composed by elements that are difficult to separate. This difficulty arises from the relevant interactions that take place between components. This lack of separability is at odds with the classical scientific method - which has been used since the times of Galileo, Newton, Descartes, and Laplace - and has also influenced philosophy and engineering. In recent decades, the scientific study of complexity and complex systems has proposed a paradigm shift in science and philosophy, proposing novel methods that take into account relevant interactions.

  8. Photofunctional hybrids of rare earth complexes covalently bonded to ZnO core-shell nanoparticle substrate through polymer linkage.

    Science.gov (United States)

    Shao, Yan-Fei; Yan, Bing

    2012-06-28

    A novel series of multi-component hybrids are assembled based on rare earth coordinated to rare earth ion (Eu(3+), Tb(3+), Sm(3+), Dy(3+)) complex systems and ZnO nanocomposites through three different ester units (ethyl methacrylate (EMA), 2-hydroxyethyl methacrylate (HEMA) and 2,2,3,4,4,4-hexafluorobutyl methacrylate (HFMA)) as functional polymer linkages. Methacrylic-group-modified ZnO nanoparticles (designated ZnO-MAA) are synthesized based on the reaction between zinc methacrylate and LiOH with the molar ratio 1 : 3.5 via sol-gel process. The final hybrid materials are prepared by introducing rare earth complexes into ZnO-MAA matrix via addition polymerization reaction in the presence of benzoyl peroxide (BPO) as the initiator. The detailed characterization and luminescence of these hybrid materials are discussed. It is found that ZnO-MAA-HEMA/EMA/HFBMA-RE-phen hybrid systems have effective intramolecular energy transfer process and exhibit longer lifetime and higher quantum efficiency.

  9. An integrated profile of natural fractures in gas-bearing shale complex (Pomerania, Poland): based on structural profiling of oriented core and borehole logging data.

    Science.gov (United States)

    Bobek, Kinga; Jarosiński, Marek; Stadtmuller, Marek; Pachytel, Radomir; Lis-Śledziona, Anita

    2016-04-01

    Natural fractures in gas-bearing shales has significant impact on reservoir stimulation and increase of exploitation. Density of natural fractures and their orientation in respect to the maximum horizontal stress are crucial for propagation of technological hydraulic fractures. Having access to continuous borehole core profile and modern geophysical logging from several wells in the Pomeranian part of the Early Paleozoic Baltic Basin (Poland) we were able to compare the consistency of structural interpretation of several data sets. Although, final aim of our research is to optimize the method of fracture network reconstruction on a reservoir scale, at a recent stage we were focused on quantitative characterization of tectonic structures in a direct vicinity of boreholes. The data we have, cover several hundred meters long profiles of boreholes from the Ordovician and Silurian shale complexes. Combining different sets of data we broaden the scale of observation from borehole core (5 cm radius), through XRMI scan of a borehole wall (10 cm radius), up to penetration of a signal of an acoustic dipole logging (several tens of cm range). At the borehole core we examined the natural tectonic structures and mechanically significant features, like: mineral veins, fractured veins, bare fractures, slickensides, fault zones, stylolites, bedding plane and mechanically contrasting layers. We have also noticed drilling-induced features like centerline fractures and core disking, controlled by a recent tectonic stress. We have measured the orientation of fractures, their size, aperture and spacing and also describe the character of veins and tried to determine the stress regime responsible for fault slippage and fracture propagation. Wide range of analyzed features allowed us to discriminate fracture sets and reconstruct tectonic evolution of the complex. The most typical for analyzed shale complexes are steep and vertical strata-bound fractures that create an orthogonal joint

  10. Using the MMPI to assess reported cognitive disturbances and somatization as a core feature of complex PTSD.

    Science.gov (United States)

    Reinhard, Matthew J; Wolf, Gregory; Cozolino, Louis

    2010-01-01

    Minnesota Multiphasic Personality Inventory (MMPI) clinical scales as well as 4 sets of MMPI items known to be sensitive to neurological dysfunction (closed head injury, cerebrovascular disorder) were administered to survivors of childhood physical and/or sexual abuse and to non-abused adults. As predicted, relative to the comparison group of psychiatric patients, the abused participants scored significantly higher on Scale 8 (Schizophrenia) and on all 4 sets of items associated with neurological dysfunction. The results suggest that early abuse/trauma is associated with cognitive disturbances and somatization. Findings appear to support the conceptualization of these psychophysical experiences as a central part of what is often called "complex posttraumatic stress disorder." Limitations and suggestions for further study are discussed.

  11. A tale of two eras: Pliocene-Pleistocene unroofing of Cenozoic and late Archean zircons from active metamorphic core complexes, Solomon Sea, Papua New Guinea

    Science.gov (United States)

    Baldwin, Suzanne L.; Ireland, Trevor R.

    1995-11-01

    U/Pb ion microprobe analyses of zircons from gneisses and granodiorites exposed in the D'Entrecasteaux Islands, and from conglomerate sections of the Goodenough No. 1 well in the adjacent Trobriand Basin, provide constraints on the age of magmatism, peak metamorphism, and nature of rocks unroofed during initial stages of metamorphic core complex formation in the Solomon Sea. The youngest populations of zircons from felsic gneisses and granodiorites indicate late Pliocene 206Pb*/238U ages. No inherited zircons were identified in the granodiorites, and the 206Pb*/238U ages (1.65 ± 0.18 Ma; 1.98 ± 0.08 Ma [2σ]) are interpreted as crystallization ages. These synkinematically emplaced granodiorites, intruded into actively extending continental crust, are some of the youngest known granitoids currently exposed at the Earth' surface. Zircon ages from felsic gneisses (2.63 ± 0.16 Ma; 2.72 ± 0.28 Ma [2σ]) are interpreted to date zircon growth subsequent to eclogite facies metamorphism. Felsic gneiss samples also contained zircon xenocrysts from Cretaceous-Miocene protoliths. In striking contrast, zircons from igneous and metamorphic clasts from the Goodenough No. 1 well indicate a single population with a 207Pb*/206/Pb* age of 2781 ± 9 Ma (2σ). We speculate that they are derived from basement rocks unroofed during initial stages of development of the D&Entrecasteaux metamorphic core complexes. These results provide the first direct evidence for the existence of Archean protoliths in the basement rocks of southeastern Papua New Guinea.

  12. Dike emplacement, footwall rotation, and the transition from magmatic to tectonic extension in the Whipple Mountains metamorphic core complex, southeastern California

    Science.gov (United States)

    Gans, Phillip B.; Gentry, Beau J.

    2016-11-01

    The Chambers Well dike swarm and associated plutonic/volcanic rocks in the western footwall of the Whipple Detachment Fault (WDF) provide key insight into the evolution of this metamorphic core complex. New structural and geochronologic data suggest that the western 12-15 km of exposed footwall is steeply tilted to the SW, providing a cross-sectional view of the upper crust, from the Miocene erosion surface to the top of the coeval mylonitization. Ages and compositions of dikes are indistinguishable from adjacent thick volcanic successions. Several kilometers of early Miocene extension ( 20.5 to 19.0 Ma) were accommodated by magmatic accretion but transitioned to rapid extensional faulting and tilting at 19.0-18.5 Ma. The subhorizontal WDF in this area initiated as a northeast dipping high-angle (50-60°) normal fault that breached the surface locally, not in a breakaway tens of kilometers to the west. Large-scale tilting and differential uplift of the western footwall was in part coeval with mylonitization and dike emplacement and was accomplished by block rotation in the hanging wall of additional normal faults, isostatic uplift, and flow of lower crust from beneath less extended regions to the west. The WDF is likely a composite surface with a western segment that had ceased moving by 18.5 Ma, cut by successively younger and steeper fault(s) to the east. Perhaps, the most important difference between seismogenic high-angle normal faults and low-angle "detachment faults" characteristic of metamorphic core complexes is one of magnitude and rate of total accumulated slip, not of initial failure conditions.

  13. Repair pathways independent of the Fanconi anemia nuclear core complex play a predominant role in mitigating formaldehyde-induced DNA damage

    Energy Technology Data Exchange (ETDEWEB)

    Noda, Taichi [Department of Biology, School of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521 (Japan); Department of Dermatology, School of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521 (Japan); Takahashi, Akihisa [Department of Biology, School of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521 (Japan); Kondo, Natsuko [Particle Radiation Oncology Research Center, Research Reactor Institute, Kyoto University, Kumatori-cho, Sennan-gun, Osaka 590-0494 (Japan); Mori, Eiichiro [Department of Biology, School of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521 (Japan); Okamoto, Noritomo [Department of Otorhinolaryngology, School of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521 (Japan); Nakagawa, Yosuke [Department of Oral and Maxillofacial Surgery, School of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521 (Japan); Ohnishi, Ken [Department of Biology, Ibaraki Prefectual University of Health Sciences, 4669-2 Ami, Ami-mati, Inasiki-gun, Ibaraki 300-0394 (Japan); Zdzienicka, Malgorzata Z. [Department of Molecular Cell Genetics, Collegium Medicum in Bydgoszcz, Nicolaus-Copernicus-University in Torun, ul. Sklodowskiej-Curie 9, 85-094 Bydgoszcz (Poland); Thompson, Larry H. [Biosciences and Biotechnology Division, L452, Lawrence Livermore National Laboratory, P.O. Box 808, Livermore, CA 94551-0808 (United States); Helleday, Thomas [Gray Institute for Radiation Oncology and Biology, University of Oxford, Old Road Campus Research Building, Off Roosevelt Drive, Oxford, OX3 7DQ (United Kingdom); Department of Genetics, Microbiology and Toxicology Stockholm University, SE-106 91 Stockholm (Sweden); Asada, Hideo [Department of Dermatology, School of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521 (Japan); and others

    2011-01-07

    The role of the Fanconi anemia (FA) repair pathway for DNA damage induced by formaldehyde was examined in the work described here. The following cell types were used: mouse embryonic fibroblast cell lines FANCA{sup -/-}, FANCC{sup -/-}, FANCA{sup -/-}C{sup -/-}, FANCD2{sup -/-} and their parental cells, the Chinese hamster cell lines FANCD1 mutant (mt), FANCGmt, their revertant cells, and the corresponding wild-type (wt) cells. Cell survival rates were determined with colony formation assays after formaldehyde treatment. DNA double strand breaks (DSBs) were detected with an immunocytochemical {gamma}H2AX-staining assay. Although the sensitivity of FANCA{sup -/-}, FANCC{sup -/-} and FANCA{sup -/-}C{sup -/-} cells to formaldehyde was comparable to that of proficient cells, FANCD1mt, FANCGmt and FANCD2{sup -/-} cells were more sensitive to formaldehyde than the corresponding proficient cells. It was found that homologous recombination (HR) repair was induced by formaldehyde. In addition, {gamma}H2AX foci in FANCD1mt cells persisted for longer times than in FANCD1wt cells. These findings suggest that formaldehyde-induced DSBs are repaired by HR through the FA repair pathway which is independent of the FA nuclear core complex. -- Research highlights: {yields} We examined to clarify the repair pathways of formaldehyde-induced DNA damage. Formaldehyde induces DNA double strand breaks (DSBs). {yields} DSBs are repaired through the Fanconi anemia (FA) repair pathway. {yields} This pathway is independent of the FA nuclear core complex. {yields} We also found that homologous recombination repair was induced by formaldehyde.

  14. From contemporary art to core clinical skills: observation, interpretation, and meaning-making in a complex environment.

    Science.gov (United States)

    Schaff, Pamela B; Isken, Suzanne; Tager, Robert M

    2011-10-01

    Many medical schools have incorporated experiences with representational or figurative art into the curriculum in an effort to improve learners' powers of observation, visual diagnostic skills, and pattern recognition skills or to enhance communication skills, foster teamwork, and/or improve empathy. The Keck School of Medicine of the University of Southern California has partnered with Los Angeles' Museum of Contemporary Art to design an educational experience with the goal of honing students' abilities to observe, describe, and interpret complex information. The authors discovered that through a constructivist approach to viewing and discussing nonrepresentational, contemporary art, students were able not only to apply their observational and interpretive skills in a safe, nonclinical setting but also to accept the facts that ambiguity is inherent to art, life, and clinical experience and that there can be more than one answer to many questions. This intervention, entailing extensive guided inquiry, collaborative thinking, and process work, has allowed students and faculty to reflect on the parallel processes at work in clinical practice and art interpretation. In patient encounters, physicians (and physicians-in-training) begin with attention and observation, continue with multiple interpretations of that which they observe, move to sorting through often ambiguous evidence, proceed to collaboration within a community of observers, and finally move to consensus and direction for action. In the worlds of both art and medicine, individuals imagine experiences beyond their own and test hypotheses by integrating their own prior knowledge and intuition and by comparing their evidence with that of others.

  15. Complex

    African Journals Online (AJOL)

    CLEMENT O BEWAJI

    Schiff bases and their complex compounds have been studied for their .... establishing coordination of the N–(2 – hydroxybenzyl) - L - α - valine Schiff base ..... (1967); “Spectrophotometric Identification of Organic Compounds”, Willey, New.

  16. Loosening After Acetabular Revision

    DEFF Research Database (Denmark)

    Beckmann, Nicholas A.; Weiss, Stefan; Klotz, Matthias C.M.;

    2014-01-01

    The best method of revision acetabular arthroplasty remains unclear. Consequently, we reviewed the literature on the treatment of revision acetabular arthroplasty using revision rings (1541 cases; mean follow-up (FU) 5.7 years) and Trabecular Metal, or TM, implants (1959 cases; mean FU 3.7 years)...

  17. Insights into the photoprotective switch of the major light-harvesting complex II (LHCII): a preserved core of arginine-glutamate interlocked helices complemented by adjustable loops.

    Science.gov (United States)

    Sunku, Kiran; de Groot, Huub J M; Pandit, Anjali

    2013-07-05

    Light-harvesting antennae of the LHC family form transmembrane three-helix bundles of which two helices are interlocked by conserved arginine-glutamate (Arg-Glu) ion pairs that form ligation sites for chlorophylls. The antenna proteins of photosystem II have an intriguing dual function. In excess light, they can switch their conformation from a light-harvesting into a photoprotective state, in which the excess and harmful excitation energies are safely dissipated as heat. Here we applied magic angle spinning NMR and selective Arg isotope enrichment as a noninvasive method to analyze the Arg structures of the major light-harvesting complex II (LHCII). The conformations of the Arg residues that interlock helix A and B appear to be preserved in the light-harvesting and photoprotective state. Several Arg residues have very downfield-shifted proton NMR responses, indicating that they stabilize the complex by strong hydrogen bonds. For the Arg Cα chemical shifts, differences are observed between LHCII in the active, light-harvesting and in the photoprotective, quenched state. These differences are attributed to a conformational change of the Arg residue in the stromal loop region. We conclude that the interlocked helices of LHCII form a rigid core. Consequently, the LHCII conformational switch does not involve changes in A/B helix tilting but likely involves rearrangements of the loops and helical segments close to the stromal and lumenal ends.

  18. Core-shell magnetite-silica composite nanoparticles enhancing DNA damage induced by a photoactive platinum-diimine complex in red light.

    Science.gov (United States)

    Zhang, Zhigang; Chai, Aiyun

    2012-12-01

    Lack of solubility under physiological conditions poses an additional risk for toxicity and side effects for intravenous delivery of the photodynamic therapeutic agent in vivo. Employing magnetite-silica composite nanoparticles as carriers of the photodynamic therapeutic agents may be a promising way to solve the problem. In this study, core-shell magnetite-silica composite nanoparticles were prepared by a sol-gel method, and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy and dynamic light scattering, then they were used as carriers of a photoactive platinum diimine complex. The interactions of the photosensitizer-loaded magnetic composite nanoparticles with DNA in red light were monitored by agarose-gel electrophoresis. The results suggest that high doses of magnetite-silica composite nanoparticles might facilitate the transformation of covalently closed circular (ccc)-DNA band to open circular (oc)-DNA band though they are harmless to DNA at their low concentrations, therefore enhancing the extent of DNA damage caused by the metal complex in red light.

  19. The Core Subunit of A Chromatin-Remodeling Complex, ZmCHB101, Plays Essential Roles in Maize Growth and Development.

    Science.gov (United States)

    Yu, Xiaoming; Jiang, Lili; Wu, Rui; Meng, Xinchao; Zhang, Ai; Li, Ning; Xia, Qiong; Qi, Xin; Pang, Jinsong; Xu, Zheng-Yi; Liu, Bao

    2016-12-05

    ATP-dependent chromatin remodeling complexes play essential roles in the regulation of diverse biological processes by formulating a DNA template that is accessible to the general transcription apparatus. Although the function of chromatin remodelers in plant development has been studied in A. thaliana, how it affects growth and development of major crops (e.g., maize) remains uninvestigated. Combining genetic, genomic and bioinformatic analyses, we show here that the maize core subunit of chromatin remodeling complex, ZmCHB101, plays essential roles in growth and development of maize at both vegetative and reproductive stages. Independent ZmCHB101 RNA interference plant lines displayed abaxially curling leaf phenotype due to increase of bulliform cell numbers, and showed impaired development of tassel and cob. RNA-seq-based transcriptome profiling revealed that ZmCHB101 dictated transcriptional reprogramming of a significant set of genes involved in plant development, photosynthesis, metabolic regulation, stress response and gene expressional regulation. Intriguingly, we found that ZmCHB101 was required for maintaining normal nucleosome density and 45 S rDNA compaction. Our findings suggest that the SWI3 protein, ZmCHB101, plays pivotal roles in maize normal growth and development via regulation of chromatin structure.

  20. The early Cretaceous orogen-scale Dabieshan metamorphic core complex: implications for extensional collapse of the Triassic HP-UHP orogenic belt in east-central China

    Science.gov (United States)

    Ji, Wenbin; Lin, Wei; Faure, Michel; Shi, Yonghong; Wang, Qingchen

    2016-03-01

    The Dabieshan massif is famous as a portion of the world's largest HP-UHP metamorphic belt in east-central China that was built by the Triassic North-South China collision. The central domain of the Dabieshan massif is occupied by a huge migmatite-cored dome [i.e., the central Dabieshan dome (CDD)]. Origin of this domal structure remains controversial. Synthesizing previous and our new structural and geochronological data, we define the Cretaceous Dabieshan as an orogen-scale metamorphic core complex (MCC) with a multistage history. Onset of lithospheric extension in the Dabieshan area occurred as early as the commencement of crustal anatexis at the earliest Cretaceous (ca. 145 Ma), which was followed by primary (early-stage) detachment during 142-130 Ma. The central Dabieshan complex in the footwall and surrounding detachment faults recorded a consistently top-to-the-NW shearing. It is thus inferred that the primary detachment was initiated from a flat-lying detachment zone at the middle crust level. Removal of the orogenic root by delamination at ca. 130 Ma came into the extensional climax, and subsequently isostatic rebound resulted in rapid doming. Along with exhumation of the footwall, the mid-crustal detachment zone had been warped as shear zones around the CDD. After 120 Ma, the detachment system probably experienced a migration accommodated to the crustal adjustment, which led to secondary (late-stage) detachment with localized ductile shearing at ca. 110 Ma. The migmatite-gneiss with HP/UHP relicts in the CDD (i.e., the central Dabieshan complex) was product of the Cretaceous crustal anatexis that consumed the deep-seated part of the HP-UHP slices and the underlying para-autochthonous basement. Compared with the contemporaneous MCCs widely developed along the eastern margin of the Eurasian continent, we proposed that occurrence of the Dabieshan MCC shares the same tectonic setting as the "destruction of the North China craton". However, geodynamic trigger

  1. The early Cretaceous orogen-scale Dabieshan metamorphic core complex: implications for extensional collapse of the Triassic HP-UHP orogenic belt in east-central China

    Science.gov (United States)

    Ji, Wenbin; Lin, Wei; Faure, Michel; Shi, Yonghong; Wang, Qingchen

    2017-06-01

    The Dabieshan massif is famous as a portion of the world's largest HP-UHP metamorphic belt in east-central China that was built by the Triassic North-South China collision. The central domain of the Dabieshan massif is occupied by a huge migmatite-cored dome [i.e., the central Dabieshan dome (CDD)]. Origin of this domal structure remains controversial. Synthesizing previous and our new structural and geochronological data, we define the Cretaceous Dabieshan as an orogen-scale metamorphic core complex (MCC) with a multistage history. Onset of lithospheric extension in the Dabieshan area occurred as early as the commencement of crustal anatexis at the earliest Cretaceous (ca. 145 Ma), which was followed by primary (early-stage) detachment during 142-130 Ma. The central Dabieshan complex in the footwall and surrounding detachment faults recorded a consistently top-to-the-NW shearing. It is thus inferred that the primary detachment was initiated from a flat-lying detachment zone at the middle crust level. Removal of the orogenic root by delamination at ca. 130 Ma came into the extensional climax, and subsequently isostatic rebound resulted in rapid doming. Along with exhumation of the footwall, the mid-crustal detachment zone had been warped as shear zones around the CDD. After 120 Ma, the detachment system probably experienced a migration accommodated to the crustal adjustment, which led to secondary (late-stage) detachment with localized ductile shearing at ca. 110 Ma. The migmatite-gneiss with HP/UHP relicts in the CDD (i.e., the central Dabieshan complex) was product of the Cretaceous crustal anatexis that consumed the deep-seated part of the HP-UHP slices and the underlying para-autochthonous basement. Compared with the contemporaneous MCCs widely developed along the eastern margin of the Eurasian continent, we proposed that occurrence of the Dabieshan MCC shares the same tectonic setting as the "destruction of the North China craton". However, geodynamic trigger

  2. Internal deformation in layered Zechstein-III K-Mg salts. Structures formed by complex deformation and high contrasts in viscosity observed in drill cores.

    Science.gov (United States)

    Raith, Alexander; Urai, Janos L.

    2016-04-01

    During the evaporation of a massive salt body, alternations of interrupted and full evaporation sequences can form a complex layering of different lithologies. Viscosity contrasts of up to five orders of magnitude between these different lithologies are possible in this environment. During the late stage of an evaporation cycle potassium and magnesium (K-Mg) salts are precipitated. These K-Mg salts are of economic interest but also a known drilling hazard due to their very low viscosity. How up to 200m thick layers of these evaporites affect salt deformation at different scales is not well known. A better understanding of salt tectonics with extreme mechanical stratification is needed for better exploration and production of potassium-magnesium salts and to predict the internal structure of potential nuclear waste repositories in salt. To gain a better understanding of the internal deformation of these layers we analyzed K-Mg salt rich drill cores out of the Zechstein III-1b subunit from the Veendam Pillow 10 km southeast of Groningen, near the city Veendam in the NE Netherlands. The study area has a complex geological history with multiple tectonic phases of extension and compression forming internal deformation in the pillow but also conserving most of the original layering. Beside halite the most common minerals in the ZIII-1b are carnallite, kieserite, anhydrite and bischofite alternating in thin layers of simple composition. Seismic interpretation revealed that the internal structure of the Veendam Pillow shows areas, in which the K-Mg salt rich ZIII 1b layer is much thicker than elsewhere, as a result of salt deformation. The internal structure of the ZIII-1b on the other hand, remains unknown. The core analysis shows a strong strain concentration in the weaker Bischofite (MgCl2*6H20) and Carnallite (KMgCl3*6H20) rich layers producing tectonic breccias and highly strained layers completely overprinting the original layering. Layers formed by alternating beds

  3. Geometry and thermal structure of the Menderes Massif Core Complex (Western Turkey), implications for thermal evolution of Hellenic subduction zone

    Science.gov (United States)

    Roche, Vincent; Jolivet, Laurent; Guillou-Frottier, Laurent; Tuduri, Johann; Bouchot, Vincent; Beccaletto, Laurent; Lahfid, Abdeltif

    2016-04-01

    The eastern Mediterranean region is one of the most promising geothermal areas, with more than 250 geothermal fields discovered in Turkey (Parlaktuna, 2013), in a region of active tectonics and volcanism. Although the potential of these deep geothermal resources has not been systematically investigated yet, the geothermal activity of the western Turkey area is the most recent signature of the high heat flow (120-140 mW/m²; Aydin, 2005, from Teczan, 1995). Based on Turkish data, 2084 MWt are being utilized for direct applications and most of the energy originates from the Menderes Massif (Baba et al., 2015). This large-scale thermal anomaly at the surface is correlated to a long wavelength east-west increase of surface heat flow that could reflect the thermal state of Aegean subduction zone at depth. In order to better understand and characterize the possible connections between large-scale mantle dynamics and surface processes in space and time, we study the structure and thermal evolution of the Menderes Massif. Both the acceleration of the Aegean extension in the Middle Miocene and the recent escape of Anatolia have been proposed to result from several slab tearing events, the first one being located below western Turkey and the Eastern Aegean Sea. These events have triggered the formation of metamorphic complexes with contrasted exhumation P-T paths. While the extension in the Aegean domain is well-characterized with high-temperature domes in the center and east, the succession of several metamorphic events in the Menderes Massif and their significance in terms of geodynamics is still debated. Hence, the exhumation history is key to understanding the temporal and spatial distribution of the thermal signature of the Hellenic slab and its tearing/detachment. The Menderes Massif displays a large variety of metamorphic facies, from the Barrovian type metamorphism in the Eocene (the Main Menderes Metamorphism) to the coeval (?) HP-LT metamorphism on the southernmost

  4. Proteomics Core

    Data.gov (United States)

    Federal Laboratory Consortium — Proteomics Core is the central resource for mass spectrometry based proteomics within the NHLBI. The Core staff help collaborators design proteomics experiments in a...

  5. Proteomics Core

    Data.gov (United States)

    Federal Laboratory Consortium — Proteomics Core is the central resource for mass spectrometry based proteomics within the NHLBI. The Core staff help collaborators design proteomics experiments in...

  6. The timing of tertiary metamorphism and deformation in the Albion-Raft River-Grouse Creek metamorphic core complex, Utah and Idaho

    Science.gov (United States)

    Strickland, A.; Miller, E.L.; Wooden, J.L.

    2011-01-01

    The Albion-Raft River-Grouse Creek metamorphic core complex of southern Idaho and northern Utah exposes 2.56-Ga orthogneisses and Neoproterozoic metasedimentary rocks that were intruded by 32-25-Ma granitic plutons. Pluton emplacement was contemporaneous with peak metamorphism, ductile thinning of the country rocks, and top-to-thewest, normal-sense shear along the Middle Mountain shear zone. Monazite and zircon from an attenuated stratigraphic section in the Middle Mountain were dated with U-Pb, using a SHRIMP-RG (reverse geometry) ion microprobe. Zircons from the deformed Archean gneiss preserve a crystallization age of 2532 ?? 33 Ma, while monazites range from 32.6 ?? 0.6 to 27.1 ?? 0.6 Ma. In the schist of the Upper Narrows, detrital zircons lack metamorphic overgrowths, and monazites produced discordant U-Pb ages that range from 52.8 ?? 0.6 to 37.5 ?? 0.3 Ma. From the structurally and stratigraphically highest unit sampled, the schist of Stevens Spring, narrow metamorphic rims on detrital zircons yield ages from 140-110 Ma, and monazite grains contained cores that yield an age of 141 ??2 Ma, whereas rims and some whole grains ranged from 35.5 ?? 0.5 to 30.0 ?? 0.4 Ma. A boudinaged pegmatite exposed in Basin Creek is deformed by the Middle Mountains shear zone and yields a monazite age of 27.6 ?? 0.2 Ma. We interpret these data to indicate two periods of monazite and metamorphic zircon growth: a poorly preserved Early Cretaceous period (???140 Ma) that is strongly overprinted by Oligocene metamorphism (???32-27 Ma) related to regional plutonism and extension. ?? 2011 by The University of Chicago.

  7. Reflections on Core Curriculum, Mission, and Catholic Identity in Our Time

    Science.gov (United States)

    Killen, Patricia O'Connell

    2015-01-01

    Reviewing and revising core curricula at Catholic colleges and universities is often fraught, primarily because the core symbolizes so centrally these institutions' identities. Situating core revision as one dimension of a larger shared task in which all Catholic institutions are engaged today-living into and articulating the meaning of being…

  8. Single-molecule magnets: a family of MnIII/CeIV complexes with a [Mn8CeO8]12+ core.

    Science.gov (United States)

    Mishra, Abhudaya; Tasiopoulos, Anastasios J; Wernsdorfer, Wolfgang; Moushi, Eleni E; Moulton, Brian; Zaworotko, Michael J; Abboud, Khalil A; Christou, George

    2008-06-02

    Four heterometallic, enneanuclear Mn8Ce clusters [Mn8CeO8(O2CMe)12(H2O)4] (4), [Mn8CeO8(O2CMe)12(py)4] (5), [Mn8CeO8(O2CPh)12(MeCN)4] [Mn8CeO8(O2CPh)12(dioxane)4] (6), and [Mn8CeO8(O2CCHPh2)12(H2O)4] (7) have been prepared by various methods. Their cores are essentially isostructural and comprise a nonplanar, saddlelike [MnIII8O8]8+ loop containing a central CeIV ion attached to the eight micro3-O2- ions. Peripheral ligation around the [Mn8CeO8]12+ core is provided by eight micro- and four micro3-O2CR- groups. Terminal ligation on four MnIII atoms is provided by H2O in 4 and 7, pyridine in 5, and MeCN/dioxane in 6. Solid-state magnetic susceptibility studies, fits of dc magnetization vs field and temperature data, and in-phase ac susceptibility studies in a zero dc field have established that complexes 4, 5, and 7 possess S=16, S=4 or 5, and S=6+/-1 spin ground states, respectively, but in all cases there are very low-lying excited states. The large variation in the ground-state spins for this isostructural family is rationalized as due to a combination of weak exchange interactions between the constituent MnIII atoms, and the presence of both nearest-neighbor and next-nearest-interactions of comparable magnitudes. Magnetization vs applied dc field sweeps on single crystals of 4.4H2O and 7.4H2O.3MeCN.2CH2Cl2 down to 0.04 K have established that these two complexes are new single-molecule magnets (SMMs). The former also shows an exchange-bias, a perturbation of its single-molecule properties from very weak intermolecular interactions mediated by hydrogen-bonding interactions with lattice-water molecules of crystallization.

  9. A benzene-core trinuclear GdIII complex: towards the optimization of relaxivity for MRI contrast agent applications at high magnetic field.

    Science.gov (United States)

    Livramento, João Bruno; Helm, Lothar; Sour, Angélique; O'Neil, Conlin; Merbach, André E; Tóth, Eva

    2008-03-07

    A novel ligand, H(12)L, based on a trimethylbenzene core bearing three methylenediethylenetriamine-N,N,N'',N''-tetraacetate moieties (-CH(2)DTTA(4-)) for Gd(3+) chelation has been synthesized, and its trinuclear Gd(3+) complex [Gd(3)L(H(2)O)(6)](3-) investigated with respect to MRI contrast agent applications. A multiple-field, variable-temperature (17)O NMR and proton relaxivity study on [Gd(3)L(H(2)O)(6)](3-) yielded the parameters characterizing water exchange and rotational dynamics. On the basis of the (17)O chemical shifts, bishydration of Gd(3+) could be evidenced. The water exchange rate, k(ex)(298)=9.0+/-3.0 s(-1) is around twice as high as k(ex)(298) of the commercial [Gd(DTPA)(H(2)O)](2-) and comparable to those on analogous Gd(3+)-DTTA chelates. Despite the relatively small size of the complex, the rotational dynamics had to be described with the Lipari-Szabo approach, by separating global and local motions. The difference between the local and global rotational correlation times, tau(lO)(298)=170+/-10 ps and tau(gO)(298)=540+/-100 ps respectively, shows that [Gd(3)L(H(2)O)(6)](3-) is not fully rigid; its flexibility originates from the CH(2) linker between the benzene core and the poly(amino carboxylate) moiety. As a consequence of the two inner-sphere water molecules per Gd(3+), their close to optimal exchange rate and the appropriate size and limited flexibility of the molecule, [Gd(3)L(H(2)O)(6)](3-) has remarkable proton relaxivities when compared with commercial contrast agents, particularly at high magnetic fields (r(1)=21.6, 17.0 and 10.7 mM(-1)s(-1) at 60, 200 and 400 MHz respectively, at 25 degrees C; r(1) is the paramagnetic enhancement of the longitudinal water proton relaxation rate, referred to 1 mM concentration of Gd(3+)).

  10. 豫西地区变质核杂岩的基本特征及其对金矿床的控制%Metamorphic core complex and its controlling role of gold deposits in Western Henan

    Institute of Scientific and Technical Information of China (English)

    孙卫志; 李磊; 谢劲松; 刘德民; 张灯堂; 杨小芬

    2013-01-01

    There exists one EW extending metamorphic core complex belt in Western Henan , which is formed from west to east by Xiaoqinling metamorphic core complex .Xiaoshan metamorphic core complex and Xiong'ershan metamorphic core complex .Xiao Qinling gold field ,Xiaoshan gold field and Xiong'ershan gold field in Western Henan Province are corresponding to Xiao Qinling metamorphic core complex .Xiaoshan metamorphic core complex and Xiong'ershan metamorphic core complex in space .The gold deposits are hosted in the different levels of the metamor-phic core complexes'detachment faults .After collecting and documenting the precise radiometric age date ,the metallo-genisis of gold deposits focuses on two periods 133-122 Ma(the main period) and 115.3-114.34 Ma,which are consistent with the metamorphic core complex territorially SEE-NWW extending phase ( 135 -123 Ma ) and the late breakdown phase(116 Ma).This illustrates that the territorially SEE-NWW extending phase parallel to the orogenic belt is the main phase for mineralization and the breakdown phase vertical to the orogenic belt is another important section for the mineralization .Through systematic study on the typical deposits'metallogenic characteristics and the for-mation,evolution and ore-controlling mechanism of metamorphic core complexes ,it can be discovered obviously that the metamorphic core complex belt plays an important role in controlling the gold deposits'types,scale,spatial distri-bution and temporal distribution in Western Henan Province ,so we can use it in looking for more gold deposits in this area.%豫西地区近EW向展布一条变质核杂岩带,自西向东由小秦岭变质核杂岩、崤山变质核杂岩和熊耳山变质核杂岩等多个变质核杂岩组成。豫西地区的小秦岭金矿田、崤山金矿田和熊耳山金矿田,在空间上分别对应于小秦岭变质核杂岩、崤山变质核杂岩和熊耳山变质核杂岩,金矿床产于变质核杂岩不同层次的拆离

  11. A revision of the history of the Colletotrichum acutatum species complex in the Nordic countries based on herbarium specimens

    DEFF Research Database (Denmark)

    Sundelin, Thomas; Strømeng, Gunn Mari; Gjærum, Halvor B.

    2015-01-01

    infect a range of important crops. As members of the C. acutatum complex are easily confused with other Colletotrichum species, molecular methods are central for the correct identification. We performed molecular analyses on 21 herbaria specimens, displaying anthracnose symptoms, collected in Norway...

  12. Sustainable Development within Planetary Boundaries: A Functional Revision of the Definition Based on the Thermodynamics of Complex Social-Ecological Systems

    Directory of Open Access Journals (Sweden)

    Bart Muys

    2013-05-01

    Full Text Available The dominant paradigm of sustainable development (SD where the environment is just the third pillar of SD has proven inadequate to keep humanity within the safe operational space determined by biophysical planetary boundaries. This implies the need for a revised definition compatible with a nested model of sustainable development, where humanity forms part of the overall social-ecological system, and that would allow more effective sustainable development goals and indicators. In this paper an alternative definition is proposed based on the thermodynamics of open systems applied to ecosystems and human systems. Both sub- systems of the global social-ecological system show in common an increased capability of buffering against disturbances as a consequence of an internal increase of order. Sustainable development is considered an optimization exercise at different scales in time and space based on monitoring the change in the exergy content and exergy dissipation of these two sub- systems of the social-ecological system. In common language it is the increase of human prosperity and well-being without loss of the structure and functioning of the ecosystem. This definition is functional as it allows the straightforward selection of quantitative indicators, discerning sustainable development from unsustainable development, unsustainable stagnation and sustainable retreat. The paper shows that the new definition is compatible with state of the art thinking on ecosystem services, the existence of regime shifts and societal transitions, and resilience. One of the largest challenges in applying the definition is our insufficient understanding of the change in ecosystem structure and function as an endpoint indicator of human action, and its effect on human prosperity and well-being. This implies the continued need to use midpoint indicators of human impact and related thresholds defining the safe operating space of the present generation with respect to

  13. Revised approach for identification of isolates within the Burkholderia cepacia complex and description of clinical isolates not assigned to any of the known genomovars.

    Science.gov (United States)

    Turton, Jane F; Arif, Nazia; Hennessy, Daneeta; Kaufmann, Mary E; Pitt, Tyrone L

    2007-09-01

    One hundred thirty-eight clinical isolates of the Burkholderia cepacia complex (Bcc) were identified using a modified strategy that involved PCR detection of the cblA gene for the ET12 lineage simultaneously with detection of the Bcc recA PCR product; recA sequence cluster analysis also was part of the strategy. Four strains could not be assigned to any of the known genomovars.

  14. Structures of mylonitic granites of the Yagan metamorphic core complex on Sino-Mongolian border——implications for its kinematics and chronology

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The mylonitic granitic plutons are extended in line-shape (L) and parallel to the regional extensional shear foliation. After being unstrained, the primary shapes of these plutons are also nearly sheet-like (S). Their fabrics are of S-L or L type. Various kinds of shear marks indicate top-to-south shear. The plutons of various stages are different in deformation intensity; however, the foliation and lineation of these rocks have the same shear sense, which suggests progressive ductile shearing. The development mechanisms of the fabrics include magmatic flow, high-temperature solidstate flow and mid-low temperature solid-state flow. All this demonstrates that these plutons are probably early synextensional tectonic plutons. Their structural features and ages suggest that the low- and mid-crustal extensional detachments might have occurred at the early stage of the development of the metamorphic core complex in the early Mesozoic. These deformations are much deeper at levels and much older than those formed at the final formation stage.

  15. Forceful Emplacement of Granitic Plutons in an Extensional Tectonic Setting: Syn-kinematic Plutons in the Yagan-Onch Hayrhan Metamorphic Core Complex

    Institute of Scientific and Technical Information of China (English)

    王涛; 郑亚东; 李天兵; 高永军; 马铭波

    2002-01-01

    It is generally considered that granitic plutons are forcefully emplaced in a compressional setting and permissively emplaced in an extensional setting. This paper, however, shows that syn-kinematic (extensional) elliptic granitic plutons in the Yagan-Onch Hayrhan metamorphic core complex (MCC) have relatively strong forceful emplacement, which are indicated by (1) concentric distribution of the rock units, (2) a strain pattern with strong strains on the margins and low strains at the centre of a pluton, and particularly (3) syn-emplacement shortening of the host rocks within the aureole. The strain analysis for the host rocks shows that the host-rock ductile shortening, I.e. Forceful emplacement, provides about 16?24% of the emplacement space for the present plutons. All these suggest that forceful emplacement occurs not only in a compressional tectonic setting, but also in an extensional setting. This study further demonstrates the significance of the multiple emplacement of granitic plutons and provides new information about the causality between granitic magmatism and the formation of the MCC and its dynamics.

  16. What do fault patterns reveal about the latest phase of extension within the Northern Snake Range metamorphic core complex, Nevada, USA?

    Science.gov (United States)

    Ismat, Zeshan; Riley, Paul; Lerback, Jory

    2016-08-01

    The Northern Snake Range is a classic example of a metamorphic core complex, Basin-and-Range province, United States. It is composed of a plastically deformed footwall and a brittlely deformed hanging wall, separated by the Northern Snake Range low-angle detachment (NSRD). Brittle deformation, however, is not confined to the hanging wall. This paper focuses on exposures in Cove Canyon, located on the SE flank of the Northern Snake Range, where penetrative, homogeneous faults are well exposed throughout the hanging wall, footwall and NSRD, and overprint early plastic deformation. These late-stage fault sets assisted Eocene-Miocene extension. Detailed analysis of the faults reveals the following: (1) The shortening direction defined by faults is similar to the shortening direction defined by the stretching lineation in the footwall mylonites, indicating that the extensional kinematic history remained unchanged as the rocks were uplifted into the elastico-frictional regime. (2) After ∼17 Ma, extension may have continued entirely within elastic-frictional regime via cataclastic flow. (3) This latest deformation phase may have been accommodated by a single, continuous event. (3) Faults within NSRD boudins indicate that deformation within the detachment zone was non-coaxial during the latest phase of extension.

  17. Three-dimensional seismic structure of the Dragon Flag oceanic core complex at the ultraslow spreading Southwest Indian Ridge (49°39'E)

    Science.gov (United States)

    Zhao, Minghui; Qiu, Xuelin; Li, Jiabiao; Sauter, Daniel; Ruan, Aiguo; Chen, John; Cannat, Mathilde; Singh, Satish; Zhang, Jiazheng; Wu, Zhenli; Niu, Xiongwei

    2013-10-01

    The Southwest Indian Ridge (SWIR) is an ultraslow spreading end-member of mid-ocean ridge system. We use air gun shooting data recorded by ocean bottom seismometers (OBS) and multibeam bathymetry to obtain a detailed three-dimensional (3-D) P wave tomographic model centered at 49°39'E near the active hydrothermal "Dragon Flag" vent. Results are presented in the form of a 3-D seismic traveltime inversion over the center and both ends of a ridge segment. We show that the crustal thickness, defined as the depth to the 7 km/s isovelocity contour, decreases systematically from the center (˜7.0-8.0 km) toward the segment ends (˜3.0-4.0 km). This variation is dominantly controlled by thickness changes in the lower crustal layer. We interpret this variation as due to focusing of the magmatic activity at the segment center. The across-axis velocity model documents a strong asymmetrical structure involving oceanic detachment faulting. A locally corrugated oceanic core complex (Dragon Flag OCC) on the southern ridge flank is characterized by high shallow crustal velocities and a strong vertical velocity gradient. We infer that this OCC may be predominantly made of gabbros. We suggest that detachment faulting is a prominent process of slow spreading oceanic crust accretion even in magmatically robust ridge sections. Hydrothermal activity at the Dragon Flag vents is located next to the detachment fault termination. We infer that the detachment fault system provides a pathway for hydrothermal convection.

  18. Proteomic analysis reveals novel proteins associated with the Plasmodium protein exporter PTEX and a loss of complex stability upon truncation of the core PTEX component, PTEX150.

    Science.gov (United States)

    Elsworth, Brendan; Sanders, Paul R; Nebl, Thomas; Batinovic, Steven; Kalanon, Ming; Nie, Catherine Q; Charnaud, Sarah C; Bullen, Hayley E; de Koning Ward, Tania F; Tilley, Leann; Crabb, Brendan S; Gilson, Paul R

    2016-11-01

    The Plasmodium translocon for exported proteins (PTEX) has been established as the machinery responsible for the translocation of all classes of exported proteins beyond the parasitophorous vacuolar membrane of the intraerythrocytic malaria parasite. Protein export, particularly in the asexual blood stage, is crucial for parasite survival as exported proteins are involved in remodelling the host cell, an essential process for nutrient uptake, waste removal and immune evasion. Here, we have truncated the conserved C-terminus of one of the essential PTEX components, PTEX150, in Plasmodium falciparum in an attempt to create mutants of reduced functionality. Parasites tolerated C-terminal truncations of up to 125 amino acids with no reduction in growth, protein export or the establishment of new permeability pathways. Quantitative proteomic approaches however revealed a decrease in other PTEX subunits associating with PTEX150 in truncation mutants, suggesting a role for the C-terminus of PTEX150 in regulating PTEX stability. Our analyses also reveal three previously unreported PTEX-associated proteins, namely PV1, Pf113 and Hsp70-x (respective PlasmoDB numbers; PF3D7_1129100, PF3D7_1420700 and PF3D7_0831700) and demonstrate that core PTEX proteins exist in various distinct multimeric forms outside the major complex.

  19. An integrated study on microtectonics, geothermometry and thermochronology of the Çataldaǧ Core Complex (NW Turkey): Implications for cooling, deformation and uplift history

    Science.gov (United States)

    Kamaci, Omer; Altunkaynak, Safak

    2017-04-01

    We present an integrated study on structure, microstructure, geothermometry and thermochronology of the Çataldaǧ Core Complex (ÇCC) in NW Turkey in order to understand the cooling, deformation and uplift mechanisms. ÇCC is formed from an Eo-Oligocene granite-gneiss-migmatite complex (GGMC) and an Early Miocene I-type granodioritic body (ÇG: Çataldaǧ granodiorite) which were exhumed as a dome-shaped core complex in the footwall of a ring-shaped low-angle detachment zone (The Çataldaǧ Detachment Fault Zone; ÇDFZ) in the Early Miocene. New U-Pb zircon (LA-ICPMS) and monazite ages of GGMC yielded magmatic ages of 33.8 and 30.1 Ma (Latest Eocene-Early Oligocene). 40Ar/39Ar muscovite, biotite and K-feldspar from the GGMC yielded the deformation age span 21.38±0,05 Ma and 20.81±0.04 Ma, which is also the emplacement age (20.84±0.13 Ma and 21.6±0.04 Ma) of ÇG. ÇDFZ is responsible for mainly top-to-the-north sense kinematic processes. The microstructural features of quartz, feldspar and mica indicate that the ÇCC has undergone continuous deformations during its cooling, from submagmatic to cataclastic conditions. Five microstructural grades have been classified under ductile (DZ) and ductile-to-brittle shear zone (SZ), according to the estimated deformation temperature and intensity of the strain. Microcline twinning, marginally replacement myrmekite and flame-perthite are predominant features for feldspar while chessboard extinction, grain boundary migration and subgrain rotation recrystallization is common for quartz in the DZ which has a deformation temperature range of >600°C to 400°C. Grain size reduction is an important factor for the ductile to brittle shear zone (SZ). Feldspar is represented by bulging recrystallization (BLG), feldspar-fish and domino-type microfracture/microfaulting and quartz show more elongated structures such as ribbons with high aspect ratios. Mineral-fish (muscovite, biotite and feldspar) structures indicate a temperature

  20. THE GEOCHEMISTRY AND AGES OF ROCKS IN THE FOOTWALL OF THE BUTULIYN-NUR AND ZAGAN METAMORPHIC CORE COMPLEXES (NORTH MONGOLIA – WESTERN TRANSBAIKALIA

    Directory of Open Access Journals (Sweden)

    T. V. Donskaya

    2015-09-01

    Full Text Available This article reviews data on ages of rocks in the footwall of the Butuliyn-Nur and Zagan metamorphic core complexes (MCC and provides new data on the geochemistry of the rock complexes. It is noted that the oldest rocks are mylonitized gneisses on rhyolites (554 Ma in the footwall of the Butuliyn-Nur MCC. The Late Permian – Triassic (249–211 Ma igneous rocks are ubiquitous in the footwall of the Butuliyn-Nur and Zagan MCC. The youngest rocks in the studied MCC are the Jurassic granitoids (178–152 Ma of the Naushki and Verhnemangirtui massifs. In the footwall of the Butuliyn-Nur and Zagan MCC, the most common are granitoids and felsic volcanic rocks (249–211 Ma with many similar geochemical characteristics, such as high alkalinity, high contents of Sr and Ba, moderate and low concentrations of Nb and Y. Considering the contents of trace elements and REE, the granitoids and the felsic volcanic rocks are similar to I-type granites. Specific compositions of these rocks suggest that they might have formed in conditions of the active continental margin of the Siberian continent over the subducting oceanic plate of the Mongol-Okhotsk Ocean. The granitoids of the Naushki and Verhnemangirtui massifs, which are the youngest of the studied rocks (178–152 Ma, also have similar geochemical characteristics. In both massif, granitoids are ferriferous, mostly alkaline rocks. By contents of both major and trace elements, they are comparable to A-type granites. Such granitoids formed in conditions of intracontinental extension while subduction was replaced by collision. Based on ages and geochemical characteristics of the rocks in the footwall of the Butuliyn-Nur and Zagan MCC, a good correlation is revealed between the studied rocks  and the rock complexes of the Transbaikalian and North-Mongolian segments of the Central Asian fold belt (CAFB, and it can thus be suggested that the regions under study may have a common evolutionary history.

  1. The cadmium–mercaptoacetic acid complex contributes to the genotoxicity of mercaptoacetic acid-coated CdSe-core quantum dots

    Directory of Open Access Journals (Sweden)

    Tang WK

    2012-05-01

    Full Text Available Weikun Tang,1 Junpeng Fan,1 Yide He,1 Bihai Huang,2 Huihui Liu,1 Daiwen Pang,2 Zhixiong Xie11College of Life Sciences, 2College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of ChinaAbstract: Quantum dots (QDs have many potential clinical and biological applications because of their advantages over traditional fluorescent dyes. However, the genotoxicity potential of QDs still remains unclear. In this paper, a plasmid-based system was designed to explore the genotoxic mechanism of QDs by detecting changes in DNA configuration and biological activities. The direct chemicobiological interactions between DNA and mercaptoacetic acid-coated CdSe-core QDs (MAA–QDs were investigated. After incubation with different concentrations of MAA–QDs (0.043, 0.13, 0.4, 1.2, and 3.6 µmol/L in the dark, the DNA conversion of the covalently closed circular (CCC DNA to the open circular (OC DNA was significantly enhanced (from 13.9% ± 2.2% to 59.9% ± 12.8% while the residual transformation activity of plasmid DNA was greatly decreased (from 80.7% ± 12.8% to 13.6% ± 0.8%, which indicated that the damages to the DNA structure and biological activities induced by MAA–QDs were concentration-dependent. The electrospray ionization mass spectrometry data suggested that the observed genotoxicity might be correlated with the cadmium–mercaptoacetic acid complex (Cd–MAA that is formed in the solution of MAA–QDs. Circular dichroism spectroscopy and transformation assay results indicated that the Cd–MAA complex might interact with DNA through the groove-binding mode and prefer binding to DNA fragments with high adenine and thymine content. Furthermore, the plasmid transformation assay could be used as an effective method to evaluate the genotoxicities of nanoparticles.Keywords: genotoxicity, MAA CdSe quantum dots, cadmium–MAA complex, transformation assay, DNA 

  2. Strategies for Revision Total Ankle Replacement

    Directory of Open Access Journals (Sweden)

    Thomas S. Roukis, DPM, PhD, FACFAS

    2014-12-01

    Full Text Available As the frequency of primary total ankle replacement (TAR continues to build, revision will become more commonplace. At present there are no “standard principles” associated with revision TAR. What is clear is that the current approaches are technically complex, fraught with complications and no one approach represents the only answer. Exchange of TAR metallic components to the same system standard or dedicated revision components are viable options with limited occurrence of complications. Explantation and conversion to custom-design long stemmed components has limited availability. Explantation and conversion to another TAR system is high-risk and has strong potential for complications. The use of metal reinforced polymethylmethacrylate cement augmentation of failed TAR systems and tibio-talo-calcaneal arthrodesis should be reserved for very select situations where other options are not possible. There is a real need for long-term survivorship following revision TAR and future efforts ought to be directed in this area.

  3. The Composite Strain Index (COSI) and Cumulative Strain Index (CUSI): methodologies for quantifying biomechanical stressors for complex tasks and job rotation using the Revised Strain Index.

    Science.gov (United States)

    Garg, Arun; Moore, J Steven; Kapellusch, Jay M

    2016-11-04

    The Composite Strain Index (COSI) quantifies biomechanical stressors for complex tasks consisting of exertions at different force levels and/or with different exertion times. The Cumulative Strain Index (CUSI) further integrates biomechanical stressors from different tasks to quantify exposure for the entire work shift. The paper provides methodologies to compute COSI and CUSI along with examples. Complex task simulation produced 169,214 distinct tasks. Use of average, time-weighted average (TWA) and peak force and COSI classified 66.9, 28.2, 100 and 38.9% of tasks as hazardous, respectively. For job rotation the simulation produced 10,920 distinct jobs. TWA COSI, peak task COSI and CUSI classified 36.5, 78.1 and 66.6% jobs as hazardous, respectively. The results suggest that the TWA approach systematically underestimates the biomechanical stressors and peak approach overestimates biomechanical stressors, both at the task and job level. It is believed that the COSI and CUSI partially address these underestimations and overestimations of biomechanical stressors. Practitioner Summary: COSI quantifies exposure when applied hand force and/or duration of that force changes during a task cycle. CUSI integrates physical exposures from job rotation. These should be valuable tools for designing and analysing tasks and job rotation to determine risk of musculoskeletal injuries.

  4. Insights into oceanic core complex formation from structural studies of IODP Hole U1473A, Expedition 360 Atlantis Bank, Southwest Indian Ridge

    Science.gov (United States)

    Deans, J. R.; Cheadle, M. J.; Ferrando, C.; Plümper, O.; Viegas, G.; Expedition 360 Scientists, I.

    2016-12-01

    Atlantis Bank formed as an oceanic core complex by exhumation along a detachment shear zone (DSZ). IODP Hole U1473A is sited on the wave-cut platform of Atlantis Bank and was cored to 809 m consisting of lower crustal gabbro, partly through this DSZ. The uppermost 600 m of the hole is a zone of intense, locally pervasive, granulite to amphibolite grade, crystal-plastic deformation. Numerous intervals of porphyroclastic to ultramylonitic gabbro, often Fe-Ti oxide-rich, reveal a protracted history of deformation. This deformation overprints primary magmatic features including igneous contacts, layering, and fabrics. From 600 mbsf to 809 mbsf, crystal-plastic deformation becomes less pervasive, but meter- to cm-scale shear zones extend to the bottom of the hole. Individual shear zones throughout the hole predominately dip between 10-50° and below 50 mbsf notably exhibit a reverse sense of shear. Amphibole veins occur mostly in the upper 300 m of the hole and crosscut the crystal-plastic foliations at high angle. These veins may both transpose and fault older crystal-plastic fabrics indicating that vein injection occurred at temperatures close to the brittle-plastic transition. Additionally, the uppermost 500 m of Hole U1473A is cut by a series of brittle faults ranging from discrete 5 cm thick cataclasites at the top to a major fault zone at 411-469 mbsf. Carbonate veins and oxidative reddish clay replacement of olivine are conspicuous in these fault zones. The distribution of deformation, and importantly the dominant reverse sense of shear recorded in Hole U1473A, is very similar to that from 400-1100 mbsf in ODP Hole 735B. Given that Hole U1473A is in the north-central part of the platform and Hole 735B is in the western margin of the platform, we suggest that preferential erosion of the central platform may have removed the upper part of the DSZ from the site of Hole U1473. If this is the case, then Hole U1473A records the crystal-plastic deformation from the

  5. Thermodynamics of electron transfer in oxygenic photosynthetic reaction centers: volume change, enthalpy, and entropy of electron-transfer reactions in manganese-depleted photosystem II core complexes.

    Science.gov (United States)

    Hou, J M; Boichenko, V A; Diner, B A; Mauzerall, D

    2001-06-19

    We have previously reported the thermodynamic data of electron transfer in photosystem I using pulsed time-resolved photoacoustics [Hou et al. (2001) Biochemistry 40, 7109-7116]. In the present work, using preparations of purified manganese-depleted photosystem II (PS II) core complexes from Synechocystis sp. PCC 6803, we have measured the DeltaV, DeltaH, and estimated TDeltaS of electron transfer on the time scale of 1 micros. At pH 6.0, the volume contraction of PS II was determined to be -9 +/- 1 A3. The thermal efficiency was found to be 52 +/- 5%, which corresponds to an enthalpy change of -0.9 +/- 0.1 eV for the formation of the state P680+Q(A-) from P680*. An unexpected volume expansion on pulse saturation of PS II was observed, which is reversible in the dark. At pH 9.0, the volume contraction, the thermal efficiency, and the enthalpy change were -3.4 +/- 0.5 A3, 37 +/- 7%, and -1.15 +/- 0.13 eV, respectively. The DeltaV of PS II, smaller than that of PS I and bacterial centers, is assigned to electrostriction and analyzed using the Drude-Nernst equation. To explain the small DeltaV for the formation of P680+Q(A-) or Y(Z*)Q(A-), we propose that fast proton transfer into a polar region is involved in this reaction. Taking the free energy of charge separation of PS II as the difference between the energy of the excited-state P680* and the difference in the redox potentials of the donor and acceptor, the apparent entropy change (TDeltaS) for charge separation of PS II is calculated to be negative, -0.1 +/- 0.1 eV at pH 6.0 (P680+Q(A-)) and -0.2 +/- 0.15 eV at pH 9.0 (Y(Z*)Q(A-)). The thermodynamic properties of electron transfer in PS II core reaction centers thus differ considerably from those of bacterial and PS I reaction centers, which have DeltaV of approximately -27 A3, DeltaH of approximately -0.4 eV, and TDeltaS of approximately +0.4 eV.

  6. Ice cores

    DEFF Research Database (Denmark)

    Svensson, Anders

    2014-01-01

    Ice cores from Antarctica, from Greenland, and from a number of smaller glaciers around the world yield a wealth of information on past climates and environments. Ice cores offer unique records on past temperatures, atmospheric composition (including greenhouse gases), volcanism, solar activity......, dustiness, and biomass burning, among others. In Antarctica, ice cores extend back more than 800,000 years before present (Jouzel et al. 2007), whereas. Greenland ice cores cover the last 130,000 years...

  7. Ice cores

    DEFF Research Database (Denmark)

    Svensson, Anders

    2014-01-01

    Ice cores from Antarctica, from Greenland, and from a number of smaller glaciers around the world yield a wealth of information on past climates and environments. Ice cores offer unique records on past temperatures, atmospheric composition (including greenhouse gases), volcanism, solar activity......, dustiness, and biomass burning, among others. In Antarctica, ice cores extend back more than 800,000 years before present (Jouzel et al. 2007), whereas. Greenland ice cores cover the last 130,000 years...

  8. 复合凝聚法制备双相核材料纳米胶囊%Preparation of Nanocapsules Containing Diphasic Core Materials by Complex Coacervation

    Institute of Scientific and Technical Information of China (English)

    王登武; 王芳; 赵晓鹏

    2012-01-01

    以明胶-阿拉伯树胶为壁材,采用复合凝聚法制备了双相核材料的纳米胶囊,其内包覆改性TiO2/四氯乙烯双相分散体系.讨论了制备过程中,各参数对纳米胶囊粒径的影响,并采用扫描电镜及透射电镜观察了纳米胶囊的粒径分布及形貌.结果表明,制备的纳米胶囊囊壁光滑、均匀,具有窄的粒径分布.双相分散体系被包覆于纳米胶囊内,且TiO2纳米颗粒粒径约为50nm.所制备的纳米胶囊平均粒径约为0.96μm,囊壁厚度约为18nm.%In this study, gelatin-gum arabic nanocapsules containing diphasic core materials were prepared by complex coacervation, during the process of which the TiO2 nanoparticles modified with stearic acid and dispersed in tetraehloroethylene (TCE) were encapsulated. The effects of the various microencapsulation parameters were also experimentally investigated. The obtained materials were characterized by raean9 of scan electron microscopy ( SEM) and transmission electron microscopy (TEM). The results indicate that the surface of the nanocapsules was smooth and clear. The diphasic dispersion was encapsulated in nanocapsules and the diameter of TiO2 nanoparticles was ~ 50 nm. The resulting nanocapsules have an average diameter of 0. 96 μm with a wall thickness of ~ 18 nm.

  9. Diachronous uplift and cooling history of the Menderes core complex, western Anatolia (Turkey), based on new Zircon (U-Th)/He ages

    Science.gov (United States)

    Baran, Zeynep Oner; Dilek, Yildirim; Stockli, Daniel

    2017-01-01

    New (U-Th)/He thermochronology data from the syn-extensional granitoids in the central part of the Menderes Massif in western Turkey reveal a minimum slip rate of 12.5 km/Myr along the Alasehir detachment ( 14° dip angle) and denudation rates between 1.75 km/Myr and 3.25 km/Myr between 4 Ma and 2 Ma. These values suggest relatively fast exhumation of the Central sub-massif, associated with cooling rates between 53 °C/Myr and 128 °C/Myr, which are higher than the estimated footwall cooling rates (60 °C/Myr to 120 °C/Myr) from the Northern sub-massif. Based on the initial crystallization ages of the syn-extensional granitoid intrusions and their exhumation-related cooling ages, our thermochronological findings suggest that the Central sub-massif in Menderes underwent accelerated uplift and faster exhumation in the latest Cenozoic than the Northern and Southern sub-massifs. This latest doming and rapid extension of the Central sub-massif was associated with the asthenospheric upwelling beneath the region and the related Na-alkaline, Kula volcanism. Our results indicate that the Menderes Massif has had a diachronous uplift and cooling history during its extensional tectonic evolution in the late Cenozoic. Thermal weakening of the young orogenic crust in western Anatolia via both lithospheric and asthenospheric melting episodes and magmatism produced higher than normal geothermal gradients and played a significant role in core complex formation.

  10. Early Cretaceous overprinting of the Mesozoic Daqing Shan fold-and-thrust belt by the Hohhot metamorphic core complex, Inner Mongolia, China

    Institute of Scientific and Technical Information of China (English)

    Gregory A. Davis; Brian J. Darby

    2010-01-01

    The Early Cretaceous Hohhot metamorphic core complex (mcc) of the Daqing Shan (Mtns.) of central Inner Mongolia is among the best exposed and most spectacular of the spatially isolated mcc's that developed within the northern edge of the North China "craton". All of these mcc's were formed within the basement of a Late Paleozoic Andean-style arc and across older Mesozoic fold-and-thrust belts of variable age and tectonic vergence. The master Hohhot detachment fault roots southwards within the southern margin of the Daqing Shan for an along-strike distance of at least 120 km. Its geometry in the range to the north is complicated by interference patterns between ( 1 ) primary, large-scale NW-SE-trending convex and concave fault corrugations and (2) secondary ENE-WSW-trending antiforms and synforms that folded the detachment in its late kinematic history. As in the Whipple Mtns. of California,the Hohhot master detachment is not of the Wernicke (1981) simple rooted type; instead, it was spawned from a mid-crustal shear zone, the top of which is preserved as a mylonitic front within Carboniferous metasedimentary rocks in its exhumed lower plate. 40Ar-39Ar dating of siliceous volcanic rocks in basal sections of now isolated supradetachment basins suggest that crustal extension began at ca. 127 Ma,although lower-plate mylonitic rocks were not exposed to erosion until after ca. 119 Ma. Essentially synchronous cooling of hornblende, biotite, and muscovite in footwall mylonitic gneisses indicates very rapid exhumation and at ca. 122-120 Ma. Contrary to several recent reports, the master detachment clearly cuts across and dismembers older, north-directed thrust sheets of the Daqing Shan foreland fold-and-thrust belt. Folded and thrust-faulted basalts within its foredeep strata are as young as 132.6 ± 2.4 Ma, thus defining within 5-6 Ma the regional tectonic transition between crustal contraction and profound crustal extension.

  11. Systematic revision of the Potamotrygon motoro (Müller & Henle, 1841 species complex in the Paraná-Paraguay basin, with description of two new ocellated species (Chondrichthyes: Myliobatiformes: Potamotrygonidae

    Directory of Open Access Journals (Sweden)

    Thiago Silva Loboda

    Full Text Available A systematic revision of the Potamotrygon motoro (Müller & Henle, 1841 species complex in the Paraná-Paraguay basin based on morphological characters was undertaken. Morphological systems analyzed include external morphology, coloration, dermal denticles, and spines, canals of the ventral lateral-line system, and skeletal components. Potamotrygon motoro is widely distributed in the Paraná-Paraguay basin and some of its diagnostic characters are: ocelli present on dorsal disc tricolored, well-defined and evenly distributed, with diameter similar or greater than eye-length; ventral coloration with relatively large whitish central region, with gray or brown area predominant on outer ventral disc margins; dermal denticles well-developed and star-shaped over central disc; labial grooves absent; monognathic heterodonty present in upper and lower jaws of adults. Potamotrygon pauckei Castex, 1963 and Potamotrygon labradori Castex, Maciel & Achenbach, 1963, are synonymized with P. motoro; Potamotrygon alba Castex, 1963, is a nomen dubium in accordance with previous authors. Additionally, two new ocellated species of Potamotrygon from the Paraná-Paraguay basin are described: Potamotrygon pantanensis, sp. nov. and Potamotrygon amandae, sp. nov. These are described and compared with P. motoro and other congeners. Potamotrygon pantanensis, sp. nov. is described from the northern Pantanal region; Potamotrygon amandae, sp. nov. is widespread in the Paraná-Paraguay basin.

  12. A revision of the Ewartia oldfieldi (Distant) species complex (Hemiptera: Cicadidae: Cicadettinae) with five new species from eastern and northern Australia.

    Science.gov (United States)

    Popple, Lindsay W

    2017-05-10

    The identity of Ewartia oldfieldi (Distant) is re-examined and this species is redescribed. Five new species belonging to the genus Ewartia Moulds are described. Ewartia oldfieldi s. str. occurs in association with wattles (Acacia spp.; Mimosaceae) with large or fleshy phyllodes growing in soils derived from sandstone and coarse-grained metasediments throughout the south-eastern third of Queensland. Ewartia roberti n. sp. is associated with wattles that possess narrow or delicate phyllodes, growing in loam soils in southern Queensland and northern New South Wales. Ewartia lapidosa n. sp. occurs in dryer inland and semi-arid areas between Croydon in northern Queensland and the Capertee Valley in central New South Wales where it occurs on various wattles growing in hard, rocky soils, including those derived from laterite and sandstone. Ewartia etesia n. sp. occurs principally on wattles growing along drainage lines in the Top End of the Northern Territory and the eastern edge of the Kimberley in Western Australia. Ewartia thamna n. sp. occurs in low, shrubby vegetation (presumably on wattles) in gravelly soils on low rises and along floodplains at the southern edge of the Top End in the Northern Territory. Ewartia carina n. sp. occurs in transitional habitats with tropical rainforest elements on the eastern edge of Cape York Peninsula in north Queensland. The distinctive, sometimes variable and typically complex calling songs specific to each of the species are illustrated and documented as part of these descriptions and comparisons.

  13. Contractor report to the Department of Energy on opportunities for integration of environmental management activities across the complex (predecisional draft). Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The US Department of Energy (DOE) Environmental Management (EM) program faces significant technical and financial challenges in cleaning up the environmental legacy of nuclear weapons production and research and development, while facing an uncertain future in obtaining the needed funding to perform this work. Many of these requirements, including State and Federal regulations and negotiated agreements, continue to be a significant contributor to EM program costs and schedules. Historically, the sites have managed their programs focusing on their individual site`s needs. While this approach maximized successes at individual sites, it has resulted in a more costly program than if more integration across the DOE system occurred. In July 1996, the DOE Assistant Secretary for EM, Al Alm, chartered a contractor led effort to perform complex-wide integration in support of the ten-year plan process to develop a suite of technically defensible, integrated alternatives to meet the EM mission. This report documents opportunities for waste and nuclear materials management integration activities in six areas: transuranic (TRU) waste, mixed low-level waste (MLLW), low-level waste (LLW), environmental restoration (ER), high-level waste (HLW), and spent nuclear fuel (SNF). The opportunities represent technically defensible solutions which reduce cost, accelerate schedules, and result in no significant increase in risk.

  14. Stripping for Revision.

    Science.gov (United States)

    Smith, Donna

    1996-01-01

    Describes a three-step process by which students are taught to revise their writing by listing each sentence on another paper, combining sentences by eliminating redundancy, reducing information to appositives, and reducing information to participles, as well as revising the reduced number of sentences by including verbals, action verbs,…

  15. Writing as Revision.

    Science.gov (United States)

    Della-Piana, Gabriel M.; Endo, George T.

    This proposal for a longitudinal experimental study with a treatment intervention focuses on the process of writing as revision. Revision refers to the process which occurs prior to and throughout the writing of a work, rather than the final editing. According to this process, the writer goes through five stages: preconceptions concerning style…

  16. Systematic revision of the marbled velvet geckos (Oedura marmorata species complex, Diplodactylidae) from the Australian arid and semi-arid zones.

    Science.gov (United States)

    Oliver, Paul M; Doughty, Paul

    2016-03-08

    Lizards restricted to rocky habitats often comprise numerous deeply divergent lineages, reflecting the disjunct nature of their preferred habitat and the capacity of rocky habitats to function as evolutionary refugia. Here we review the systematics and diversity of the predominantly saxicoline Australian marbled velvet geckos (genus Oedura) in the Australian arid and semi-arid zones using newly-gathered morphological data and previously published genetic data. Earlier work showed that four largely allopatric and genetically divergent lineages are present: Western (Pilbara and Gascoyne regions), Gulf (west and south of the Gulf of Carpentaria), Central (central ranges) and Eastern (Cooper and Darling Basins). None of these four populations are conspecific with true O. marmorata, a seperate species complex that is restricted to the Top End region of the Northern Territory. Top End forms share a short, bulbous tail whereas the other four lineages treated here possess a long, tapering tail. Morphological differences among the arid and semi-arid lineages include smaller body size, tapering lamellae and a shorter tail for the Gulf population, and a partially divided rostral scale in the Western population compared to the Central and Eastern populations. Accordingly, we resurrect O. cincta de Vis from synonymy for the Central and Eastern lineages, and regard this species as being comprised of two evolutionary significant units. We also describe the Gulf and Western lineages as new species: Oedura bella sp. nov. and O. fimbria sp. nov., respectively. We note that a predominantly arboreal lineage (the Eastern lineage of O. cincta) is more widely distributed than the other lineages and is phylogenetically nested within a saxicoline clade, but tends to have a deeper head and shorter limbs, consistent with morphological variation observed in other lizard radiations including both saxicoline and arboreal taxa.

  17. Reconstrucción del complejo areola-pezón: revisión de 60 casos Nipple-areola complex reconstruction: revision of 60 cases

    Directory of Open Access Journals (Sweden)

    R. Fernández García

    2007-06-01

    hay ninguna cuyos resultados se sobrepongan a las demás, pero sí una vez que se reconstruye la areola siendo la técnica que más satisface la del injerto inguinal. A pesar de ello, el aspecto más notable a mejorar está en la reconstrucción del pezón, dada la frecuente disconformidad de las pacientes con la proyección conseguida a lo largo del tiempo por la posible reabsorción del mismo.The creation of the nipple-areola complex is the latest time in breast reconstruction, transforming the reconstruction of the breast mound into a real breast. We have to consider the reconstruction of the areola and the nipple as the culmination of breast reconstruction. There are a lot of documented techniques for nipple-areola complex reconstruction. The aim of this study is to determine the grade of psychological satisfaction of patients after this reconstruction This study was designed as a retrospective clinic review of 60 patients. After reviewing medical histories, the patients were interviewed and asked to complete a questionnaire. The most common desired aspect was to correct the absence/lost of nipple projection. However, 22% of patients answered they would not change anything regarding their reconstruction. The satisfaction with the mammary mound was excellent or good for 68%, normal for 23 % and poor for 9 %. On the other hand, satisfaction for the nippleareola complex reconstruction was excellent or good for 50%, normal for 45% and poor for 5%. There were no statistical differences among the different techniques depending on the time between the mastectomy intervention and the third reconstruction (p=0,06. For nipple reconstruction, the contralateral nipple donation technique offered more satisfaction (2.67 points and projection (7.23 points. In spite of the differences in their means, there were no statistically significant differences. Taking into account the technique used for the areola reconstruction, the donation-graft of inguinal skin was the one that

  18. Revision of the DELFIC Particle Activity Module

    Energy Technology Data Exchange (ETDEWEB)

    Hooper, David A [ORNL; Jodoin, Vincent J [ORNL

    2010-09-01

    The Defense Land Fallout Interpretive Code (DELFIC) was originally released in 1968 as a tool for modeling fallout patterns and for predicting exposure rates. Despite the continual advancement of knowledge of fission yields, decay behavior of fission products, and biological dosimetry, the decay data and logic of DELFIC have remained mostly unchanged since inception. Additionally, previous code revisions caused a loss of conservation of radioactive nuclides. In this report, a new revision of the decay database and the Particle Activity Module is introduced and explained. The database upgrades discussed are replacement of the fission yields with ENDF/B-VII data as formatted in the Oak Ridge Isotope Generation (ORIGEN) code, revised decay constants, revised exposure rate multipliers, revised decay modes and branching ratios, and revised boiling point data. Included decay logic upgrades represent a correction of a flaw in the treatment of the fission yields, extension of the logic to include more complex decay modes, conservation of nuclides (including stable nuclides) at all times, and conversion of key variables to double precision for nuclide conservation. Finally, recommended future work is discussed with an emphasis on completion of the overall radiation physics upgrade, particularly for dosimetry, induced activity, decay of the actinides, and fractionation.

  19. Gap analysis: a method to assess core competency development in the curriculum.

    Science.gov (United States)

    Fater, Kerry H

    2013-01-01

    To determine the extent to which safety and quality improvement core competency development occurs in an undergraduate nursing program. Rapid change and increased complexity of health care environments demands that health care professionals are adequately prepared to provide high quality, safe care. A gap analysis compared the present state of competency development to a desirable (ideal) state. The core competencies, Nurse of the Future Nursing Core Competencies, reflect the ideal state and represent minimal expectations for entry into practice from pre-licensure programs. Findings from the gap analysis suggest significant strengths in numerous competency domains, deficiencies in two competency domains, and areas of redundancy in the curriculum. Gap analysis provides valuable data to direct curriculum revision. Opportunities for competency development were identified, and strategies were created jointly with the practice partner, thereby enhancing relevant knowledge, attitudes, and skills nurses need for clinical practice currently and in the future.

  20. Integration of offshore seismic data, exploration wells, and onland outcrops as constraints on the tectonics and uplift age of metamorphic core complexes, eastern Papua New Guinea

    Science.gov (United States)

    Fitz, G. G.; Mann, P.; Campos Aguiniga, H.

    2009-12-01

    of the core complexes occurred during the latest Miocene-earliest Pliocene period.

  1. Constraints on the Lost City Hydrothermal System from borehole thermal data; 3-D models of heat flow and hydrothermal circulation in an oceanic core complex.

    Science.gov (United States)

    Titarenko, S.; McCaig, A. M.

    2014-12-01

    A perennial problem in near-ridge hydrothermal circulation is that the only directly measurable data to test models is often vent fluid temperature. Surface heat flow measurements may be available but without the underlying thermal structure it is not known if they are transient and affected by local hydrothermal flow, or conductive. The Atlantis Massif oceanic core complex at 30 °N on the mid-Atlantic Ridge, offers a unique opportunity to better constrain hydrothermal circulation models. The temperature profile in gabbroic rocks of IODP Hole 1309D was measured in IODPExpedition 340T, and found to be near-conductive, but with a slight inflexion at ~750 mbsf indicating downward advection of fluid above that level. The lack of deep convection is especially remarkable given that the long-lived Lost City Hydrothermal Field (LCHF) is located only 5km to the south. We have modelled hydrothermal circulation in the Massif using Comsol Multiphysics, comparing 2-D and 3-D topographic models and using temperature-dependent conductivity to give the best estimate of heatflow into the Massif. We can constrain maximum permeability in gabbro below 750 mbsf to 5e-17 m2. The thermal gradient in the upper part of the borehole can be matched with a permeability of 3e-14 m2 in a 750 m thick layer parallel to the surface of the massif, with upflow occurring in areas of high topography and downflow at the location of the borehole. However in 3-D the precise flow pattern is quite model dependent, and the thermal structure can be matched either by downflow centred on the borehole at lower permeability or centred a few hundred metres from the borehole at higher permeability. The borehole gradient is compatible with the longevity (>120 kyr) and outflow temperature (40-90 °C) of the LCHF either with a deep more permeable (1e-14 m2 to 1e-15 m2) domain beneath the vent site in 2-D or a permeable fault slot 500 to 1000m wide and parallel to the transform fault in 3-D. In both cases topography

  2. Letter of Map Revision

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The National Flood Hazard Layer (NFHL) data incorporates all Digital Flood Insurance Rate Map(DFIRM) databases published by FEMA, and any Letters Of Map Revision...

  3. Katz's revisability paradox dissolved

    NARCIS (Netherlands)

    Tamminga, Allard; Verhaegh, Sander

    2013-01-01

    Quine's holistic empiricist account of scientific inquiry can be characterized by three constitutive principles: noncontradiction, universal revisability and pragmatic ordering. We show that these constitutive principles cannot be regarded as statements within a holistic empiricist's scientific theo

  4. Katz's revisability paradox dissolved

    NARCIS (Netherlands)

    Tamminga, Allard; Verhaegh, Sander

    2013-01-01

    Quine's holistic empiricist account of scientific inquiry can be characterized by three constitutive principles: noncontradiction, universal revisability and pragmatic ordering. We show that these constitutive principles cannot be regarded as statements within a holistic empiricist's scientific

  5. Revised Total Coliform Rule

    Science.gov (United States)

    The Revised Total Coliform Rule (RTCR) aims to increase public health protection through the reduction of potential pathways for fecal contamination in the distribution system of a public water system (PWS).

  6. Transformer core

    NARCIS (Netherlands)

    Mehendale, A.; Hagedoorn, Wouter; Lötters, Joost Conrad

    2010-01-01

    A transformer core includes a stack of a plurality of planar core plates of a magnetically permeable material, which plates each consist of a first and a second sub-part that together enclose at least one opening. The sub-parts can be fitted together via contact faces that are located on either side

  7. Transformer core

    NARCIS (Netherlands)

    Mehendale, A.; Hagedoorn, Wouter; Lötters, Joost Conrad

    2008-01-01

    A transformer core includes a stack of a plurality of planar core plates of a magnetically permeable material, which plates each consist of a first and a second sub-part that together enclose at least one opening. The sub-parts can be fitted together via contact faces that are located on either side

  8. Revising the Complex Economics of Patent Scope

    DEFF Research Database (Denmark)

    Howells, John

    Merges and Nelson claim to have provided an empirically grounded argument that pioneer patents of 'broad' scope are used to block technological development. It is widely understood in both law and economics that they have, as they claim, faulted Kitch's 'prospect theory' of patents, a theory that...... of development. This interpretation restores the credibility of Kitch's prospect theory of patent function and emphasises that the administration of the patent institution should be designed to support the prospect function of patents.......Merges and Nelson claim to have provided an empirically grounded argument that pioneer patents of 'broad' scope are used to block technological development. It is widely understood in both law and economics that they have, as they claim, faulted Kitch's 'prospect theory' of patents, a theory...... that a function of patents is to enable the coordinated development of novel technical ideas. This article is a critical review of Merges and Nelson's historical empirical evidence. I find that, first, 'broad' scope is only implicated in one of the examples cited by Merges and Nelson as supportive evidence...

  9. Physical Properties of Gabbroic Rock Exposed in Oceanic Core Complexes- New Borehole Data From IODP Hole U1473A in the Indian Ocean and Prior Mid-Atlantic Ridge Results

    Science.gov (United States)

    Blackman, D. K.; Ildefonse, B.; Abe, N.; Harding, A. J.; Guerin, G.

    2016-12-01

    IODP Expedition 360 to Atlantis Bank on the Southwest Indian Ridge obtained physical property measurements of the 800 m section drilled into the footwall of the oceanic core complex. Compressional velocity (Vp) of core samples range from 5.9-7.2 km/s throughout the hole, with no simple relation to either basic rock type or alteration. Some intervals show a local trend, for example a general increase from 6.7-7.1 km/s over the interval 280-400 mbsf, above a major fault zone at 411-462 mbsf. Below the fault zone, core sample Vp is lower on average (6.6 km/s) than it is in the upper part of the hole (6.8 km/s). Some of this decrease is due to locally greater alteration, but higher oxide content also contributes. Borehole logs show lower Vp shallower than 400 m (6.3-6.4 km/s) and close match to olivine gabbro values below the fault zone, due to higher alteration levels and greater shallow fracturing. Local trends of decreasing Vp, over 10's of m correspond to increasing sample porosity within veined or fractured intervals. Porosities of core in Hole U1473A are low overall (fairly constant values ( 6.7 km/s) at greater depths, interrupted by a highly altered olivine-rich troctolite interval 1080-1200 mbsf where velocity is up to 1 km/s slower. New analysis of seismic anisotropy based on sonic logs does not show any systematic signature for either core complex, but there are a few intervals up to 10 m thick where anisotropy due to local deformation or dominant fracture direction may be indicated. The new and prior borehole data will be presented in the context of available geophysical, lithologic and alteration results.

  10. Ice Cores

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Records of past temperature, precipitation, atmospheric trace gases, and other aspects of climate and environment derived from ice cores drilled on glaciers and ice...

  11. Core BPEL

    DEFF Research Database (Denmark)

    Hallwyl, Tim; Højsgaard, Espen

    extensions. Combined with the fact that the language definition does not provide a formal semantics, it is an arduous task to work formally with the language (e.g. to give an implementation). In this paper we identify a core subset of the language, called Core BPEL, which has fewer and simpler constructs......, does not allow omissions, and does not contain ignorable elements. We do so by identifying syntactic sugar, including default values, and ignorable elements in WS-BPEL. The analysis results in a translation from the full language to the core subset. Thus, we reduce the effort needed for working...... formally with WS-BPEL, as one, without loss of generality, need only consider the much simpler Core BPEL. This report may also be viewed as an addendum to the WS-BPEL standard specification, which clarifies the WS-BPEL syntax and presents the essential elements of the language in a more concise way...

  12. Core BPEL

    DEFF Research Database (Denmark)

    Hallwyl, Tim; Højsgaard, Espen

    extensions. Combined with the fact that the language definition does not provide a formal semantics, it is an arduous task to work formally with the language (e.g. to give an implementation). In this paper we identify a core subset of the language, called Core BPEL, which has fewer and simpler constructs......, does not allow omissions, and does not contain ignorable elements. We do so by identifying syntactic sugar, including default values, and ignorable elements in WS-BPEL. The analysis results in a translation from the full language to the core subset. Thus, we reduce the effort needed for working...... formally with WS-BPEL, as one, without loss of generality, need only consider the much simpler Core BPEL. This report may also be viewed as an addendum to the WS-BPEL standard specification, which clarifies the WS-BPEL syntax and presents the essential elements of the language in a more concise way...

  13. Core benefits

    National Research Council Canada - National Science Library

    Keith, Brian W

    2010-01-01

    This SPEC Kit explores the core employment benefits of retirement, and life, health, and other insurance -benefits that are typically decided by the parent institution and often have significant governmental regulation...

  14. Toward a Comprehensive Framework for Evaluating the Core Integration Features of Enterprise Integration Middleware Technologies

    Directory of Open Access Journals (Sweden)

    Hossein Moradi

    2013-01-01

    Full Text Available To achieve greater automation of their business processes, organizations face the challenge of integrating disparate systems. In attempting to overcome this problem, organizations are turning to different kinds of enterprise integration. Implementing enterprise integration is a complex task involving both technological and business challenges and requires appropriate middleware technologies. Different enterprise integration solutions provide various functions and features which lead to the complexity of their evaluation process. To overcome this complexity, appropriate tools for evaluating the core integration features of enterprise integration solutions is required. This paper proposes a new comprehensive framework for evaluating the core integration features of both intra-enterprise and inter-enterprise Integration's enabling technologies, which simplify the process of evaluating the requirements met by enterprise integration middleware technologies.The proposed framework for evaluating the core integration features of enterprise integration middleware technologies was enhanced using the structural and conceptual aspects of previous frameworks. It offers a new schema for which various enterprise integration middleware technologies are categorized in different classifications and are evaluated based on their supporting level for the core integration features' criteria. These criteria include the functional and supporting features. The proposed framework, which is a revised version of our previous framework in this area, has developed the scope, structure and content of the mentioned framework.

  15. Revising and editing for translators

    CERN Document Server

    Mossop, Brian

    2014-01-01

    Revising and Editing for Translators provides guidance and learning materials for translation students learning to edit texts written by others, and professional translators wishing to improve their self-revision ability or learning to revise the work of others. Editing is understood as making corrections and improvements to texts, with particular attention to tailoring them to the given readership. Revising is this same task applied to draft translations. The linguistic work of editors and revisers is related to the professional situations in which they work. Mossop offers in-depth coverage of a wide range of topics, including copyediting, style editing, structural editing, checking for consistency, revising procedures and principles, and translation quality assessment. This third edition provides extended coverage of computer aids for revisers, and of the different degrees of revision suited to different texts. The inclusion of suggested activities and exercises, numerous real-world examples, a proposed gra...

  16. The thesaurus review, renaissance, and revision

    CERN Document Server

    Roe, Sandra K

    2013-01-01

    Use this single source to uncover the origin and development of the thesaurus! The Thesaurus: Review, Renaissance, and Revision examines the historical development of the thesaurus and the standards employed for thesaurus construction. This book provides both the history of thesauri and tutorials on usage to increase your understanding of thesaurus creation, use, and evaluation. This reference tool offers essential information on thesauri in the digital environment, including Web sites, databases, and software. For 50 years, the thesaurus has been a core reference book; The Thesaurus: Review,

  17. Revision without ordinals

    NARCIS (Netherlands)

    Rivello, Edoardo

    2013-01-01

    We show that Herzberger’s and Gupta’s revision theories of truth can be recast in purely inductive terms, without any appeal neither to the transfinite ordinal numbers nor to the axiom of Choice. The result is presented in an abstract and general setting, emphasising both its validity for a wide ran

  18. Revision of Oxandra (Annonaceae)

    NARCIS (Netherlands)

    Junikka, L.; Maas, P.J.M.; Maas-van de Kamer, H.; Westra, L.Y.Th.

    2016-01-01

    A taxonomic revision is given of the Neotropical genus Oxandra (Annonaceae). Within the genus 27 species are recognized, 4 of which are new to science. Most of the species are occurring in tropical South America, whereas a few (6) are found in Mexico and Central America and two in the West Indies

  19. Revision of Pachycentria (Melastomataceae)

    NARCIS (Netherlands)

    Clausing, Gudrun

    2000-01-01

    A revision of Pachycentria Blume, which includes the monotypic Pogonanthera Blume, is presented. Pachycentria comprises eight species and one subspecies. Two species, P. vogelkopensis and P. hanseniana, are newly described. The genus is distinguished from other genera in the Medinillinae by a small

  20. Revision of the Sarcospermataceae

    NARCIS (Netherlands)

    Lam, H.J.; Varossieau, W.W.

    1938-01-01

    The genus Sarcosperma was excluded from the Sapotaceae by the first-named writer in 1925, the group being considered as of family rank. In 1926 the same author published a concise and fragmentary revision of the monotypic order, in which two new Malaysian species were described. The continental spec

  1. Encapsulated Fe3O4 /Ag complexed cores in hollow gold nanoshells for enhanced theranostic magnetic resonance imaging and photothermal therapy.

    Science.gov (United States)

    Lin, Adam Y; Young, Joseph K; Nixon, Ariel V; Drezek, Rebekah A

    2014-08-27

    Designed and fabrication of a novel magnetic hollow gold nanoshell complexes that incorporates iron oxide nanoparticles in the hollow interior. The combined effect of the smaller IONPs improved the overall magnetic properties of the design and MRI contrast capability. The overall complex could be synthesized in the range of 60-80 nm in diameter while still having a plasmonic peak in the near infrared region.

  2. Synthetic Tuning of Redox, Spectroscopic, and Photophysical Properties of {Mo6I8}(4+) Core Cluster Complexes by Terminal Carboxylate Ligands.

    Science.gov (United States)

    Mikhailov, Maxim A; Brylev, Konstantin A; Abramov, Pavel A; Sakuda, Eri; Akagi, Soichiro; Ito, Akitaka; Kitamura, Noboru; Sokolov, Maxim N

    2016-09-06

    The reactions between the tetra-n-butylammonium salt of [{Mo6I8}I6](2-) and silver carboxylates RCOOAg (R = CH3 (1), C(CH3)3 (2), α-C4H3O (3), C6H5 (4), α-C10H7 (5), or C2F5 (6)) in CH2Cl2 afforded new carboxylate complexes [{Mo6I8}(RCOO)6](2-). The complexes were characterized by X-ray single-crystal diffraction and elemental analysis, cyclic/differential pulse voltammetry, and IR, NMR, and UV-visible spectroscopies. The emission properties of the complexes 1-6, and those of the earlier reported complexes with R = CF3 (7) and n-C3F7 (8), were studied both in acetonitrile solution and in the solid state. In deaerated CH3CN at 298 K, all of the complexes 1-8 exhibit intense and long-lived emission with the quantum yield and lifetime being 0.48-0.73 and 283-359 μs, respectively. The oxidation (Eox)/reduction (Ered) potentials of the complexes correlate linearly with the pKa value of the terminal carboxylate ligands L = RCOO (pKa(L)). Reflecting the pKa(L) dependences of Eox/Ered, the emission energy (νem) of the complexes was also shown to correlate with pKa(L). The present study successfully demonstrates synthetic tuning of the redox, spectroscopic, and photophysical characteristics of a {Mo6I8}(4+)-based cluster complex with pKa(L).

  3. Matrix metalloproteinase-10/TIMP-2 structure and analyses define conserved core interactions and diverse exosite interactions in MMP/TIMP complexes.

    Science.gov (United States)

    Batra, Jyotica; Soares, Alexei S; Mehner, Christine; Radisky, Evette S

    2013-01-01

    Matrix metalloproteinases (MMPs) play central roles in vertebrate tissue development, remodeling, and repair. The endogenous tissue inhibitors of metalloproteinases (TIMPs) regulate proteolytic activity by binding tightly to the MMP active site. While each of the four TIMPs can inhibit most MMPs, binding data reveal tremendous heterogeneity in affinities of different TIMP/MMP pairs, and the structural features that differentiate stronger from weaker complexes are poorly understood. Here we report the crystal structure of the comparatively weakly bound human MMP-10/TIMP-2 complex at 2.1 Å resolution. Comparison with previously reported structures of MMP-3/TIMP-1, MT1-MMP/TIMP-2, MMP-13/TIMP-2, and MMP-10/TIMP-1 complexes offers insights into the structural basis of binding selectivity. Our analyses identify a group of highly conserved contacts at the heart of MMP/TIMP complexes that define the conserved mechanism of inhibition, as well as a second category of diverse adventitious contacts at the periphery of the interfaces. The AB loop of the TIMP N-terminal domain and the contact loops of the TIMP C-terminal domain form highly variable peripheral contacts that can be considered as separate exosite interactions. In some complexes these exosite contacts are extensive, while in other complexes the AB loop or C-terminal domain contacts are greatly reduced and appear to contribute little to complex stability. Our data suggest that exosite interactions can enhance MMP/TIMP binding, although in the relatively weakly bound MMP-10/TIMP-2 complex they are not well optimized to do so. Formation of highly variable exosite interactions may provide a general mechanism by which TIMPs are fine-tuned for distinct regulatory roles in biology.

  4. The structure of a mixed GluR2 ligand-binding core dimer in complex with (S)-glutamate and the antagonist (S)-NS1209

    DEFF Research Database (Denmark)

    Kasper, Christina; Pickering, Darryl S; Mirza, Osman;

    2006-01-01

    ] in one protomer and the endogenous ligand (S)-glutamate in the other. (S)-NS1209 stabilises an even more open conformation of the D1 and D2 domains of the ligand-binding core than that of the apo structure due to steric hindrance. This is the first time ligand-induced hyperextension of the binding...... domains has been observed. (S)-NS1209 adopts a novel binding mode, including hydrogen bonding to Tyr450 and Gly451 of D1. Parts of (S)-NS1209 occupy new areas of the GluR2 ligand-binding cleft, and bind near residues that are not conserved among receptor subtypes. The affinities of (RS)-NS1209 at the Glu....... The thermodynamics of binding of the antagonists (S)-NS1209, DNQX and (S)-ATPO to the GluR2 ligand-binding core have been determined by displacement isothermal titration calorimetry. The displacement of (S)-glutamate by all antagonists was shown to be driven by enthalpy....

  5. Trinuclear manganese complexes of unsymmetrical polypodal diamino N3O3 ligands with an unusual [Mn3(μ-OR)4]5+ triangular core: synthesis, characterization, and catalase activity.

    Science.gov (United States)

    Ledesma, Gabriela N; Anxolabéhère-Mallart, Elodie; Rivière, Eric; Mallet-Ladeira, Sonia; Hureau, Christelle; Signorella, Sandra R

    2014-03-01

    Two new tri-Mn(III) complexes of general formula [Mn3L2(μ-OH)(OAc)]ClO4 (H3L = 1-[N-(2-pyridylmethyl),N-(2-hydroxybenzyl)amino]-3-[N'-(2-hydroxybenzyl),N'-(4-X-benzyl)amino]propan-2-ol; 1ClO4, X = Me; 2ClO4, X = H) have been prepared and characterized. X-ray diffraction analysis of 1ClO4 reveals that the complex cation possesses a Mn3(μ-alkoxo)2(μ-hydroxo)(μ-phenoxo)(4+) core, with the three Mn atoms bound to two fully deprotonated N3O3 chelating L(3-), one exogenous acetato ligand, and one hydroxo bridge, the structure of which is retained upon dissolution in acetonitrile or methanol. The three Mn atoms occupy the vertices of a nearly isosceles triangle (Mn1···Mn3 = 3.6374(12) Å, Mn2···Mn3 3.5583(13) Å, and Mn1···Mn2 3.2400(12) Å), with one substitution-labile site on the apical Mn ion occupied by terminally bound monodentate acetate. Temperature-dependent magnetic susceptibility studies indicate the presence of predominant antiferromagnetic intramolecular interactions between Mn(III) ions in 1ClO4. Complexes 1ClO4 and 2ClO4 decompose H2O2 at comparable rates upon initial binding of peroxide through acetate substitution, with retention of core structure during catalysis. Kinetic and spectroscopic studies suggest that these complexes employ the [Mn-(μ-oxo/aquo)-Mn](4+) moiety to activate peroxide, with the additional (μ-alkoxo)(μ-phenoxo)Mn(μ-alkoxo) metallobridge carrying out a structural function.

  6. Low-temperature thermochronologic constraints on cooling and exhumation trends along conjugate margins, within core complexes and eclogite-bearing gneiss domes of the Woodlark rift system of eastern Papua New Guinea

    Science.gov (United States)

    Fitzgerald, P. G.; Baldwin, S.; Bermudez, M. A.; Miller, S. R.; Webb, L. E.; Little, T.

    2012-12-01

    In eastern Papua New Guinea, active sea-floor spreading within the Woodlark Basin has been propagating westward since at least 6 Ma into heterogeneous crust of the Woodlark Rift. The seafloor spreading system divides the northern conjugate margin (Woodlark Rise) from the southern margin (Pocklington Rise). West of the seafloor spreading rift-tip are high-standing extensional gneiss domes and core complexes of the D'Entrecasteaux Islands (DEI). Domes comprise amphibolite and eclogite-facies gneisses, and Pleistocene granitoid intrusions. Flanked by mylonitic shear zone carapaces and normal faults, the domes are juxtaposed against an upper plate that includes ultramafic rocks and gabbro, correlated with the Papuan ultramafic belt. Petrologic and structural evidence from the DEI has been interpreted as evidence for diapiric ascent of the largely felsic domes, with thermo-mechanical modeling proposing (U)HP exhumation in terms of diapiric flow aided by propagating extension, with feedback between the two. Core complexes lacking evidence for diapiric-aided exhumation include the Prevost Range (eastern Normanby Island), Dayman Dome (Papuan Peninsula), and Misima Island (southern conjugate margin). Thermochronology is being applied to understand the thermal and exhumation history, and hence help constrain mechanisms of (U)HP exhumation. AFT and AHe ages from samples near sea-level along conjugate margins and DEI range from ca. 12 Ma to Goodenough Island, the western-most and highest-standing dome. On Goodenough Island, samples from the core zone have AFT ages from ~3 - test the relative roles of buoyancy and normal faulting during exhumation of eclogite-bearing domes within the Woodlark rift system.

  7. Preparation and electrochemical properties of core-shell carbon coated Mn–Sn complex metal oxide as anode materials for lithium-ion batteries

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Ruixue [Key Laboratory of Lithium Battery Materials of Jiangsu Province, Institute of chemical power sources, Soochow University, Suzhou 215006 (China); Fang, Guoqing; Liu, Weiwei [Key Laboratory of Lithium Battery Materials of Jiangsu Province, Institute of chemical power sources, Soochow University, Suzhou 215006 (China); Changzhou Institute of Energy Storage Materials and Devices, Changzhou 213000 (China); Xia, Bingbo; Sun, Hongdan; Zheng, Junwei [Key Laboratory of Lithium Battery Materials of Jiangsu Province, Institute of chemical power sources, Soochow University, Suzhou 215006 (China); Li, Decheng, E-mail: lidecheng@suda.edu.cn [Key Laboratory of Lithium Battery Materials of Jiangsu Province, Institute of chemical power sources, Soochow University, Suzhou 215006 (China)

    2014-02-15

    In this study, we synthesized a carbon coated Mn–Sn metal oxide composite with core-shell structure (MTO@C) via a simple glucose hydrothermal reaction and subsequent carbonization approach. When the MTO@C composite was applied as an anode material for lithium-ion batteries, it maintained a reversible capacity of 409 mA h g{sup −1} after 200 cycles at a current density of 100 mA g{sup −1}. The uniformed and continuous carbon layer formed on the MTO nanoparticles, effectively buffered the volumetric change of the active material and increased electronic conductivity, which thus prolonged the cycling performance of the MTO@C electrode.

  8. Rhinoplasty for the multiply revised nose.

    Science.gov (United States)

    Foda, Hossam M T

    2005-01-01

    To evaluate the problems encountered on revising a multiply operated nose and the methods used in correcting such problems. The study included 50 cases presenting for revision rhinoplasty after having had 2 or more previous rhinoplasties. An external rhinoplasty approach was used in all cases. Simultaneous septal surgery was done whenever indicated. All cases were followed for a mean period of 32 months (range, 1.5-8 years). Evaluation of the surgical result depended on clinical examination, comparison of pre- and postoperative photographs, and degree of patients' satisfaction with their aesthetic and functional outcome. Functionally, 68% suffered nasal obstruction that was mainly caused by septal deviations and nasal valve problems. Aesthetically, the most common deformities of the upper two thirds of the nose included pollybeak (64%), dorsal irregularities (54%), dorsal saddle (44%), and open roof deformity (42%), whereas the deformities of lower third included depressed tip (68%), tip contour irregularities (60%), and overrotated tip (42%). Nasal grafting was necessary in all cases; usually more than 1 type of graft was used in each case. Postoperatively, 79% of the patients, with preoperative nasal obstruction, reported improved breathing; 84% were satisfied with their aesthetic result; and only 8 cases (16%) requested further revision to correct minor deformities. Revision of a multiply operated nose is a complex and technically demanding task, yet, in a good percentage of cases, aesthetic as well as functional improvement are still possible.

  9. A unique missense allele of BAF155, a core BAF chromatin remodeling complex protein, causes neural tube closure defects in mice.

    Science.gov (United States)

    Harmacek, Laura; Watkins-Chow, Dawn E; Chen, Jianfu; Jones, Kenneth L; Pavan, William J; Salbaum, J Michael; Niswander, Lee

    2014-05-01

    Failure of embryonic neural tube closure results in the second most common class of birth defects known as neural tube defects (NTDs). While NTDs are likely the result of complex multigenic dysfunction, it is not known whether polymorphisms in epigenetic regulators may be risk factors for NTDs. Here we characterized Baf155(msp3) , a unique ENU-induced allele in mice. Homozygous Baf155(mps3) embryos exhibit highly penetrant exencephaly, allowing us to investigate the roles of an assembled, but malfunctional BAF chromatin remodeling complex in vivo at the time of neural tube closure. Evidence of defects in proliferation and apoptosis were found within the neural tube. RNA-Seq analysis revealed that surprisingly few genes showed altered expression in Baf155 mutant neural tissue, given the broad epigenetic role of the BAF complex, but included genes involved in neural development and cell survival. Moreover, gene expression changes between individual mutants were variable even though the NTD was consistently observed. This suggests that inconsistent gene regulation contributes to failed neural tube closure. These results shed light on the role of the BAF complex in the process of neural tube closure and highlight the importance of studying missense alleles to understand epigenetic regulation during critical phases of development.

  10. Cambridge IGCSE mathematics core and extended

    CERN Document Server

    Pimentel, Ric

    2013-01-01

    The most cost effective and straightforward way to teach the revised syllabus, with all the core and extended content covered by a single book and accompanying free digital resources.  . This title has been written for the revised Cambridge IGCSE Mathematics (0580) syllabus, for first teaching from 2013.  . ·         Gives students the practice they require to deepen their understanding through plenty of questions. ·         Consolidates learning with unique digital resources on the CD, included free with every Student's Book.  . We are working with Cambridge International Examinations to gain

  11. Rare-earth metal methylidene complexes with Ln3(μ3-CH2)(μ3-Me)(μ2-Me)3 core structure.

    Science.gov (United States)

    Schädle, Dorothea; Meermann-Zimmermann, Melanie; Maichle-Mössmer, Cäcilia; Schädle, Christoph; Törnroos, Karl W; Anwander, Reiner

    2015-11-07

    Trinuclear rare-earth metal methylidene complexes with a Ln3(μ3-CH2)(μ3-Me)(μ2-Me)3 structural motif were synthesized by applying three protocols. Polymeric [LuMe3]n (1-Lu) reacts with the sterically demanding amine H[NSiMe3(Ar)] (Ar = C6H3iPr2-2,6) in tetrahydrofuran via methane elimination to afford isolable monomeric [NSiMe3(Ar)]LuMe2(thf)2 (4-Lu). The formation of trinuclear rare-earth metal tetramethyl methylidene complexes [NSiMe3(Ar)]3Ln3(μ3-CH2)(μ3-Me)(μ2-Me)3(thf)3 (7-Ln; Ln = Y, Ho, Lu) via reaction of [LnMe3]n (1-Ln; Ln = Y, Ho, Lu) with H[NSiMe3(Ar)] is proposed to occur via an "intermediate" species of the type [NSiMe3(Ar)]LnMe2(thf)x and subsequent C-H bond activation. Applying Lappert's concept of Lewis base-induced methylaluminate cleavage, compounds [NSiMe3(Ar)]Ln(AlMe4)2 (5-Ln; Ln = Y, La, Nd, Ho) were converted into methylidene complexes 7-Ln (Ln = Y, Nd, Ho) in the presence of tetrahydrofuran. Similarly, tetramethylgallate complex [NSiMe3(Ar)]Y(GaMe4)2 (6-Y) could be employed as a synthesis precursor for 7-Y. The molecular composition of complexes 4-Ln, 5-Ln, 6-Y and 7-Ln was confirmed by elemental analyses, FTIR spectroscopy, (1)H and (13)C NMR spectroscopy (except for holmium derivatives) and single-crystal X-ray diffraction. The Tebbe-like reactivity of methylidene complex 7-Nd with 9-fluorenone was assessed affording oxo complex [NSiMe3(Ar)]3Nd3(μ3-O)(μ2-Me)4(thf)3 (8-Nd). The synthesis of 5-Ln yielded [NSiMe3(Ar)]2Ln(AlMe4) (9-Ln; Ln = La, Nd) as minor side-products, which could be obtained in moderate yields when homoleptic Ln(AlMe4)3 were treated with two equivalents of K[NSiMe3(Ar)].

  12. Vanadium complexes having [V(IV)O](2+) and [V(V)O(2)](+) cores with binucleating dibasic tetradentate ligands: Synthesis, characterization, catalytic and antiamoebic activities.

    Science.gov (United States)

    Maurya, Mannar R; Khan, Aftab Alam; Azam, Amir; Ranjan, Samir; Mondal, Neelima; Kumar, Amit; Avecilla, Fernando; Pessoa, João Costa

    2010-02-07

    Binucleating hydrazones CH(2)(H(2)sal-bhz)(2) (I) and CH(2)(H(2)sal-fah)(2) (II), derived from 5,5'-methylbis(salicylaldehyde) and benzoylhydrazide or 2-furoylhydrazide, react with [V(IV)O(acac)(2)] to give dinuclear V(IV)O-complexes [CH(2){V(IV)O(sal-bhz)(H(2)O)}(2)] 1 and [CH(2){V(IV)O(sal-fah)(H(2)O)}(2)] 4, respectively. In the presence of KOH or CsOH.H(2)O, oxidation of 1 and 2 results in the formation of dioxidovanadium(v) complexes, K(2)[CH(2){V(V)O(2)(sal-bhz)}(2)].2H(2)O 2, K(2)[CH(2){V(V)O(2)(sal-fah)}(2)].2H(2)O 5, Cs(2)[CH(2){V(V)O(2)(sal-bhz)}(2)].2H(2)O 3 and Cs(2)[CH(2){V(V)O(2)(sal-fah)}(2)].2H(2)O 6. These complexes have also been prepared by aerial oxidation of in situ prepared oxidovanadium(iv) complexes 1 and 4. The compounds were characterized by IR, electronic, EPR, (1)H, (13)C and (51)V NMR spectroscopy, elemental analyses and thermogravimetric patterns. Single crystal X-ray analysis of 3 confirms the coordination of the ligand in the dianionic (ONO(2-)) enolate tautomeric form. The V(V)O(2)-complexes were used to catalyze the oxidative bromination of salicylaldehyde, therefore acting as functional models of vanadium dependent haloperoxidases, in aqueous H(2)O(2)/KBr in the presence of HClO(4) at room temperature. It is shown that the V(IV)O-complexes [CH(2){V(IV)O(sal-bhz)(H(2)O)}(2)] 1 and [CH(2){V(IV)O(sal-fah)(H(2)O)}(2)] 4 are catalyst precursors for the catalytic oxidation of organic sulfides using aqueous H(2)O(2). Plausible intermediates involved in these catalytic processes are established by UV-Vis, EPR and (51)V NMR studies. The vanadium complexes along with ligands I and II are also screened against HM1:1MSS strains of Entamoeba histolytica, the results showing that the IC(50) values of compounds 3 and 6 are lower than that of metronidazole. The toxicity studies against human cervical (HeLa) cancer cell line also showed that although compounds 3 and 6 are more toxic than metronidazole towards this cell line, the corresponding IC

  13. Phase Diagram of Iron, Revised-Core Temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Ahrens, T.J.; Chen, G.Q.; Holland, K.G.

    1999-01-27

    Shock-wave experiments on iron preheated to 1,573 K conducted from 14 to 73 GPa, yield new data for sound velocities of the {gamma}- and liquid-phases. Melting was observed in the highest pressure ({approximately} 71 {+-} 2 GPa) experiments at calculated shock temperatures of 2,775 {+-} 160 K. This single crossing of the {gamma}-liquid boundary measured here agrees closely with the {gamma}-iron melting line determined by Boehler [1993], Saxena et al. [1993], and Jephcoat and Besedin [1997]. This {gamma}-iron melting curve is {approximately} 300 C lower than that of Shen et al. [1998b] at 80 GPa.

  14. Ethylene oxide and Acetaldehyde in hot cores

    CERN Document Server

    Occhiogrosso, A; Herbst, E; Viti, S; Ward, M D; Price, S D; Brown, W A

    2014-01-01

    [Abridged] Ethylene oxide and its isomer acetaldehyde are important complex organic molecules because of their potential role in the formation of amino acids. Despite the fact that acetaldehyde is ubiquitous in the interstellar medium, ethylene oxide has not yet been detected in cold sources. We aim to understand the chemistry of the formation and loss of ethylene oxide in hot and cold interstellar objects (i) by including in a revised gas-grain network some recent experimental results on grain surfaces and (ii) by comparison with the chemical behaviour of its isomer, acetaldehyde. We test the code for the case of a hot core. The model allows us to predict the gaseous and solid ethylene oxide abundances during a cooling-down phase prior to star formation and during the subsequent warm-up phase. We can therefore predict at what temperatures ethylene oxide forms on grain surfaces and at what temperature it starts to desorb into the gas phase. The model reproduces the observed gaseous abundances of ethylene oxid...

  15. Core Java

    CERN Document Server

    Horstmann, Cay S

    2013-01-01

    Fully updated to reflect Java SE 7 language changes, Core Java™, Volume I—Fundamentals, Ninth Edition, is the definitive guide to the Java platform. Designed for serious programmers, this reliable, unbiased, no-nonsense tutorial illuminates key Java language and library features with thoroughly tested code examples. As in previous editions, all code is easy to understand, reflects modern best practices, and is specifically designed to help jumpstart your projects. Volume I quickly brings you up-to-speed on Java SE 7 core language enhancements, including the diamond operator, improved resource handling, and catching of multiple exceptions. All of the code examples have been updated to reflect these enhancements, and complete descriptions of new SE 7 features are integrated with insightful explanations of fundamental Java concepts.

  16. 2 : 2 Fe(III): ligand and "adamantane core" 4 : 2 Fe(III): ligand (hydr)oxo complexes of an acyclic ditopic ligand

    DEFF Research Database (Denmark)

    Ghiladi, Morten; Larsen, Frank B.; McKenzie, Christine J.;

    2005-01-01

    A bis-hydroxo-bridged diiron(III) complex and a bis-mu-oxo-bis-mu-hydroxo-bridged tetrairon( III) complex are isolated from the reaction of 2,6-bis((N, N'-bis-(2-picolyl) amino) methyl)-4-tert-butylphenol (Hbpbp) with iron perchlorate in acidic and neutral solutions respectively. The X......-ray structure of the dinuclear complex [{( Hbpbp) Fe(mu-OH)}(2)](ClO4)(4) center dot 2C(3)H(6)O ( 1 center dot 2C(3)H(6)O) shows that only one of the metal-binding cavities of each ligand is occupied by an iron( III) atom and two [Fe(Hbpbp)](3+) units are linked together by two hydroxo bridging groups to form...... bond lengths of the two different octahedral iron sites: Fe -mu-OH, 1.953( 5), 2.013( 5) angstrom and Fe-mu-O, 1.803( 5), 1.802( 5) angstrom. The difference in ligand environment is too small for allowing Mossbauer spectroscopy to distinguish between the two crystallographically independent Fe sites...

  17. Human embryonic stem cell-derived hematopoietic cells maintain core epigenetic machinery of the polycomb group/Trithorax Group complexes distinctly from functional adult hematopoietic stem cells.

    Science.gov (United States)

    Schnerch, Angelique; Lee, Jung Bok; Graham, Monica; Guezguez, Borhane; Bhatia, Mickie

    2013-01-01

    Hematopoietic cells derived from human embryonic stem cells (hESCs) have a number of potential utilities, including the modeling of hematological disorders in vitro, whereas the use for cell replacement therapies has proved to be a loftier goal. This is due to the failure of differentiated hematopoietic cells, derived from human pluripotent stem cells (hPSCs), to functionally recapitulate the in vivo properties of bona fide adult hematopoietic stem/progenitor cells (HSPCs). To better understand the limitations of differentiation programming at the molecular level, we have utilized differential gene expression analysis of highly purified cells that are enriched for hematopoietic repopulating activity across embryonic, fetal, and adult human samples, including in vivo explants of human HSPCs 8-weeks post-transplantation. We reveal that hESC-derived hematopoietic progenitor cells (eHPCs) fail to express critical transcription factors which are known to govern self-renewal and myeloid/lymphoid development and instead retain the expression of Polycomb Group (PcG) and Trithorax Group (TrxG) factors which are more prevalent in embryonic cell types that include EZH1 and ASH1L, respectively. These molecular profiles indicate that the differential expression of the core epigenetic machinery comprising PcGs/TrxGs in eHPCs may serve as previously unexplored molecular targets that direct hematopoietic differentiation of PSCs toward functional HSPCs in humans.

  18. Core-binding factor β increases the affinity between human Cullin 5 and HIV-1 Vif within an E3 ligase complex.

    Science.gov (United States)

    Salter, Jason D; Lippa, Geoffrey M; Belashov, Ivan A; Wedekind, Joseph E

    2012-11-06

    HIV-1 Vif masquerades as a receptor for a cellular E3 ligase harboring Elongin B, Elongin C, and Cullin 5 (EloB/C/Cul5) proteins that facilitate degradation of the antiretroviral factor APOBEC3G (A3G). This Vif-mediated activity requires human core-binding factor β (CBFβ) in contrast to cellular substrate receptors. We observed calorimetrically that Cul5 binds tighter to full-length Vif((1-192))/EloB/C/CBFβ (K(d) = 5 ± 2 nM) than to Vif((95-192))/EloB/C (K(d) = 327 ± 40 nM), which cannot bind CBFβ. A comparison of heat capacity changes supports a model in which CBFβ prestabilizes Vif((1-192)) relative to Vif((95-192)), consistent with a stronger interaction of Cul5 with Vif's C-terminal Zn(2+)-binding motif. An additional interface between Cul5 and an N-terminal region of Vif appears to be plausible, which has therapeutic design implications.

  19. Relativistic frozen core potential scheme with relaxation of core electrons

    Science.gov (United States)

    Nakajima, Yuya; Seino, Junji; Hayami, Masao; Nakai, Hiromi

    2016-10-01

    This letter proposes a relaxation scheme for core electrons based on the frozen core potential method at the infinite-order Douglas-Kroll-Hess level, called FCP-CR. The core electrons are self-consistently relaxed using frozen molecular valence potentials after the valence SCF calculation is performed. The efficiency of FCP-CR is confirmed by calculations of gold clusters. Furthermore, FCP-CR reproduces the results of the all-electron method for the energies of coinage metal dimers and the core ionization energies and core level shifts of vinyl acetate and three tungsten complexes at the Hartree-Fock and/or symmetry-adapted cluster configuration interaction levels.

  20. Highly fluorescent platinum(II) organometallic complexes of perylene and perylene monoimide, with Pt σ-bonded directly to the perylene core.

    Science.gov (United States)

    Lentijo, Sergio; Miguel, Jesús A; Espinet, Pablo

    2010-10-18

    3-Bromoperylene (BrPer) or N-(2,5-di-tert-butylphenyl)-9-bromo-perylene-3,4-dicarboximide (BrPMI) react with [Pt(PEt(3))(4)] to yield trans-[PtR(PEt(3))(2)Br] (R = Per, 1a; R = PMI, 1b). Neutral and cationic perylenyl complexes containing a Pt(PEt(3))X group have been prepared from 1a,b by substitution of the Br ligand by a variety of other ligands (NCS, CN, NO(3), CN(t)Bu, PyMe). The X-ray structures of trans-[PtR(PEt(3))(2)X] (R = Per, X = NCS (2a); R = PMI, X = NO(3) (4b); R = Per, X = CN(t)Bu (5a)) show that the perylenyl fragment remains nearly planar and is arranged almost orthogonal to the coordination plane: The three molecules appear as individual entities in the solid state, with no π-π stacking of perylenyl rings. Each platinum complex exhibits fluorescence associated to the perylene or PMI fragments with emission quantum yields, in solution at room temperature, in the range 0.30-0.80 and emission lifetimes ∼4 ns, but with significantly different emission maxima, by influence of the X ligands on Pt. The similarity of the overall luminescence spectra of these metalated complexes with the perylene or PMI strongly suggests a perylene-dominated intraligand π-π*emissive state, metal-perturbed by interaction of the platinum fragment mostly via polarization of the Ar-Pt bond.

  1. Intervalence charge transfer transition in mixed valence complexes synthesised from RuIII(edta)- and FeII(CN)5-cores

    Indian Academy of Sciences (India)

    H C Bajaj; Atindra D Shukla; Amitava Das

    2002-08-01

    Intervalence charge transfer properties were studied for a set of mixed valence complexes incorporating Ru(III) and Fe(II)-centres linked by various saturated and unsaturated bridging ligands (BL). Studies reveal that degree of ground state electronic interaction and coupling between Ru(III) and Fe(II)-centres can be attenuated by changing the nature of the bridging ligand. Further, inclusion of the bridging ligand with interrupted -electron system in a -CD cavity initiate an optical electron transfer from Fe(II) to Ru(III) which is otherwise not observed.

  2. Synthesis and characterization of dendrimeric melamine cored [salen/salophFe(III)] and [salen/salophCr(III)] capped complexes and their magnetic behaviors

    Energy Technology Data Exchange (ETDEWEB)

    Uysal, Saban, E-mail: uysal77@hotmail.com [Department of Chemistry, Faculty of Science, Selcuk University, Campus of Alaaddin Keykubat, 42075 Selcuklu, Konya (Turkey); Koc, Ziya Erdem [Department of Chemistry, Faculty of Science, Selcuk University, Campus of Alaaddin Keykubat, 42075 Selcuklu, Konya (Turkey)

    2010-03-15

    2,4,6-Tris(4-hydroxybenzimino)-1,3,5-triazine [1] 2 has been synthesized from the reaction of 1 equiv. melamine (2,4,6-triamino-1,3,5-triazine) and 3 equiv. 4-hydroxybenzaldehyde. Then, 2,4,6-tris(4-(4,6-dichloro-1,3,5-triazine-2-yloxy)benzimino)-1,3,5-triazine 3 has been synthesized from the reaction of 1 equiv. 2 and 3 equiv. cyanuric chloride. And then, two new triazine centered dendrimeric ligands 2,4,6-tris(4-(4,6-bis(4-carboxyphenyloxy)-1,3,5-triazine-2-yloxy)benzimino) -1,3,5-triazine 4 and 2,4,6-tris(4-(4,6-bis(3,5-dicarboxyphenyloxy) -1,3,5-triazine-2-yloxy)benzimino)-1,3,5-triazine 5 have been synthesized from the reaction of 1 equiv. 3 and 6 equiv 4-hydroxybenzoic acid or 5-hydroxyisophtalic acid. Finally, eight new multinuclear Fe(III) and Cr(III) complexes involving tetradenta Schiff bases N,N'-bis(salicylidene)ethylenediamine-(salenH{sub 2}) or bis(salicylidene)-o-phenylene diamine-(salophH{sub 2}) with 4 or 5 have been synthesized and characterized by means of elemental analysis, {sup 1}H NMR, FT-IR spectroscopy, thermal analyses and magnetic susceptibility measurements. The complexes can also be characterized as low-spin distorted octahedral Fe(III) and Cr(III) bridged by carboxylic acids.

  3. Synthesis, molecular structures and phase transition studies on benzothiazole-cored Schiff bases with their Cu(II) and Pd(II) complexes: Crystal structure of (E)-6-methoxy-2-(4-octyloxy-2-hydroxybenzylideneamino)benzothiazole

    Science.gov (United States)

    Yeap, Guan-Yeow; Heng, Boon-Teck; Faradiana, Nur; Zulkifly, Raihana; Ito, Masato M.; Tanabe, Makoto; Takeuchi, Daisuke

    2012-03-01

    Two new homologous series of Cu(II) and Pd(II) complexes with benzothiazole-cored Schiff bases have been synthesised with the aim to study the mesomorphic and thermal properties of ligands upon formation of metal complexes. The molecular structure of title compounds were elucidated with the employment of FT-IR, 1D and 2D FT-NMR spectroscopic techniques. Mesomorphic and thermal behaviour of title compounds have been investigated by differential scanning calorimetry and polarising optical microscope. All the ligands are nematogenic but the corresponding Cu(II) and Pd(II) complexes crystallised in ordinary solid. The conformation of 6-methoxy-2-(4-octyloxy-2-hydroxy-benzylideneamino)benzothiazole was determined by single crystal X-ray diffraction analysis of which the title compound favours more stable (E)-6-methoxy-2-(4-octyloxy-2-hydroxybenzylideneamino)benzothiazole. Crystal structure of the title compound also revealed that the bond length of Cdbnd N (1.303 Å) in the benzothiazole rings very close to that in the exocyclic Cdbnd N linkage (1.298 Å).

  4. Core calculations of JMTR

    Energy Technology Data Exchange (ETDEWEB)

    Nagao, Yoshiharu [Japan Atomic Energy Research Inst., Oarai, Ibaraki (Japan). Oarai Research Establishment

    1998-03-01

    In material testing reactors like the JMTR (Japan Material Testing Reactor) of 50 MW in Japan Atomic Energy Research Institute, the neutron flux and neutron energy spectra of irradiated samples show complex distributions. It is necessary to assess the neutron flux and neutron energy spectra of an irradiation field by carrying out the nuclear calculation of the core for every operation cycle. In order to advance core calculation, in the JMTR, the application of MCNP to the assessment of core reactivity and neutron flux and spectra has been investigated. In this study, in order to reduce the time for calculation and variance, the comparison of the results of the calculations by the use of K code and fixed source and the use of Weight Window were investigated. As to the calculation method, the modeling of the total JMTR core, the conditions for calculation and the adopted variance reduction technique are explained. The results of calculation are shown. Significant difference was not observed in the results of neutron flux calculations according to the difference of the modeling of fuel region in the calculations by K code and fixed source. The method of assessing the results of neutron flux calculation is described. (K.I.)

  5. Two Core Systems of Dynamic Logic

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiao-jun; LI Ke-sheng; HAO Yi-jiang

    2012-01-01

    Dynamic Logic (DL) is a formal system for reasoning on the input/output behaviors of programs. Hoare Logic (HL) is the precursor of all dynamic logics known today. Two core systems of DL are Propositional Dynamic Logic (PDL) and Quantificational Dynamic Logic (QDL). PDL is an extension of propositional logic with programs and is the appropriate place to begin investigating DL. QDL can be viewed as the first-order version of PDL. Predicate Dynamic Logic (DPL) is a subsystem of QDL and can be regarded as the most basic of a hierarchy of formulas-as-programs languages. These systems constitute the main topic of this essay. The authors’ elaboration here is very brief and sketchy and with the aim of providing the readers with only the most essence of the topic on the basis of other researchers’ works. The last part is the important one in which the authors summarize the approaches of extending Dynamic Logic. The conclusions are as follows: variants of DL are obtained by reinterpreting some constructs as something else, and/or by adding rules or operators, and/or by restricting or extending or revising some constructs, and/or combining a kind of logic with another one, and/or using a comprehensive way which insights from other disciplines according to its application in various domains. In all these cases, the authors give examples to illustrate the conclusion. It is generally proposed that sometimes the introduction of a new operator or rule or construct, or the introduction of reinterpretation or restriction or extension or revision of some constructs will increase expressive power and sometimes not; sometimes it has effect on the complexity of deciding satisfiability and sometimes not. Finally, the authors sum up major aspects which we should consider during investigating a specific variant of DL. The researchers should focus on the well-formed expressions and on the validity of expressions about it with respect to standard, non-standard and syntactically

  6. A multi-isotope approach to understanding the evolution of Cenozoic magmatism in the northeastern Basin and Range: Results from igneous rocks in the Albion-Raft River-Grouse Creek metamorphic core complex

    Science.gov (United States)

    Konstantinou, A.; Strickland, A.; Miller, E. L.

    2012-12-01

    Deep crustal rocks exposed by extensional processes in metamorphic core complexes provide a unique opportunity to address the magmatic and isotopic evolution of the crust and assess the relative crust versus mantle contributions in Cenozoic igneous rocks exposed in the complexes. The Albion-Raft River-Grouse Creek metamorphic core complex exposes mid-crustal rocks that resided at depths of ~15-20 km before the onset of Cenozoic extension. Three major Cenozoic magmatic events are represented in the complex and have been studied using multiple isotopic systems (whole rock Sr and Nd coupled with the Oxygen isotopes in zircon). These three major events are: (1) 42-31 Ma intrusion of a composite plutonic complex of calc-alkaline composition that intrudes both upper crustal rocks (~5-10 km depth) and deeper rocks. (2) A 32-25 Ma plutonic complex, with evolved calc-alkaline composition that intruded in the middle crust (~12-15 km depth), and (3) A 10-8 Ma bimodal (basalt-rhyolite) suite of volcanic rocks that contain high-T anhydrous mineral assemblages erupted across the complex. The pre-extensional crust consisted of an upper crust composed primarily of Neoproterozoic through Triassic metasedimentary rocks (schist and quartzite at its base and limestone at its top). The middle crust consists of late Archean orthogneiss with evolved composition (metamorphosed peraluminous granite) with average 87Sr/86Sr40~0.800, ɛNd40~ -43.4 and δ18Ozirc ~5.7‰. The lower crust is inferred to have been composed of Precambrian intermediate composition igneous rocks with average 87Sr/86Sr40~0.750, ɛNd40~ -37.5 and δ18Ozirc ~5.9‰, and Precambrian mafic rocks with average 87Sr/86Sr40~0.717, ɛNd40~ -25 and δ18Ozirc ~7.0‰. Existing and new data indicate that the 42-31 Ma upper crustal plutonic complex ranges in isotopic composition from 87Sr/86Sri=0.709-0.712, ɛNdi=-15 to -25 and δ18Ozirc 4.7-6.5‰. The composition of the 32-25 Ma middle crustal plutonic complex ranges from 87Sr

  7. ΔpH-dependent non-photochemical quenching (qE) of excited chlorophylls in the photosystem II core complex of the freshwater cyanobacterium Synechococcus sp PCC 7942.

    Science.gov (United States)

    Stamatakis, Kostas; Papageorgiou, George C

    2014-08-01

    Light-induced and lumen acidity-dependent quenching (qE) of excited chlorophylls (Chl) in vivo has been amply documented in plants and algae, but not in cyanobacteria, using primarily the saturation pulse method of quenching analysis which is applied to continuously illuminated samples. This method is unsuitable for cyanobacteria because the background illumination elicits in them a very large Chl a fluorescence signal, due to a state 2 to state 1 transition, which masks fluorescence changes due to other causes. We investigated the qE problem in the cyanobacterium Synechococcus sp. PCC 7942 using a kinetic method (Chl a fluorescence induction) with which qE can be examined before the onset of the state 2 to state 1 transition and the attendant rise of Chl a fluorescence. Our results confirm the existence of a qE mechanism that operates on excited Chls a in Photosystem II core complexes of cyanobacteria.

  8. Revision Total Elbow Arthroplasty.

    Science.gov (United States)

    Ramirez, Miguel A; Cheung, Emilie V; Murthi, Anand M

    2017-08-01

    Despite recent technologic advances, total elbow arthroplasty has complication rates higher than that of total joint arthroplasty in other joints. With new antirheumatic treatments, the population receiving total elbow arthroplasty has shifted from patients with rheumatoid arthritis to those with posttraumatic arthritis, further compounding the high complication rate. The most common reasons for revision include infection, aseptic loosening, fracture, and component failure. Common mechanisms of total elbow arthroplasty failure include infection, aseptic loosening, fracture, component failure, and instability. Tension band fixation, allograft struts with cerclage wire, and/or plate and screw constructs can be used for fracture stabilization.

  9. MARKOWITZ STRATEGIES REVISED

    Institute of Scientific and Technical Information of China (English)

    Yan Jia-an; Zhou Xunyu

    2009-01-01

    Continuous-time Markowitz's mean-variance efficient strategies are modified by parameterizing a critical quantity. It is shown that these parameterized Markowitz strategies could reach the original mean target with arbitrarily high probabilities. This, in turn, motivates the introduction of certain stopped strategies where stock holdings are liquidated whenever the parameterized Markowitz strategies reach the present value of the mean target. The risk aspect of the revised Markowitz strategies are examined via expected discounted loss from the initial budget. A new portfolio selection model is suggested based on the results of the paper.

  10. Mineralogical and geochemical evidence for hydrothermal activity at the west wall of 12°50′N core complex (Mid-Atlantic ridge): a new ultramafic-hosted seafloor hydrothermal deposit?

    Science.gov (United States)

    Dekov, Vesselin; Boycheva, Tanya; Halenius, Ulf; Billstrom, Kjell; Kamenov, George D.; Shanks, Wayne C.; Stummeyer, Jens

    2011-01-01

    Dredging along the west wall of the core complex at 12°50′N Mid-Atlantic Ridge sampled a number of black oxyhydroxide crusts and breccias cemented by black and dark brown oxyhydroxide matrix. Black crusts found on top of basalt clasts (rubble) are mainly composed of Mn-oxides (birnessite, 10-Å manganates) with thin films of nontronite and X-ray amorphous FeOOH on their surfaces. Their chemical composition (low trace- and rare earth-element contents, high Li and Ag concentrations, rare earth element distribution patterns with negative both Ce and Eu anomalies), Sr–Nd–Pb-isotope systematic and O-isotope data suggest low-temperature (~ 20 °C) hydrothermal deposition from a diffuse vent area on the seafloor. Mineralogical, petrographic and geochemical investigations of the breccias showed the rock clasts were hydrothermally altered fragments of MORBs. Despite the substantial mineralogical changes caused by the alteration the Sr–Nd–Pb-isotope ratios have not been significantly affected by this process. The basalt clasts are cemented by dark brown and black matrix. Dark brown cement exhibits geochemical features (very low trace- and rare earth- element contents, high U concentration, rare earth element distribution pattern with high positive Eu anomaly) and Nd–Pb-isotope systematics (similar to that of MORB) suggesting that the precursor was a primary, high-temperature Fe-sulfide, which was eventually altered to goethite at ambient seawater conditions. The data presented in this work points towards the possible existence of high- and low-temperature hydrothermal activity at the west wall of the core complex at 12°50′N Mid-Atlantic Ridge. Tectonic setting at the site implies that the proposed hydrothermal field is possibly ultramafic-hosted.

  11. Revised Rules for Concrete Bridges

    DEFF Research Database (Denmark)

    Thoft-Christensen, Palle; Jensen, F. M.; Middleton, C.;

    This paper is based on research performed for the Highway Agency, London, UK under the project DPU/9/44 "Revision of Bridge Assessment Rules Based on Whole Life Performance: Concrete Bridges" It contains details of a methodology which can be used to generate Whole Life (WL) reliability profiles....... These WL reliability profiles may be used to establish revised rules for Concrete Bridges....

  12. Emotion Processes in Knowledge Revision

    Science.gov (United States)

    Trevors, Gregory J.; Kendeou, Panayiota; Butterfuss, Reese

    2017-01-01

    In recent years, a number of insights have been gained into the cognitive processes that explain how individuals overcome misconceptions and revise their previously acquired incorrect knowledge. The current study complements this line of research by investigating the moment-by-moment emotion processes that occur during knowledge revision using a…

  13. Interior Design: Revision as Focus.

    Science.gov (United States)

    Smede, Shelly D.

    2000-01-01

    Describes how the author teaches her eighth-grade students to revise their writing, providing "working revision days" in class, offering direction and structure, and thereby helping students learn how much impact going back to a piece of writing and making sweeping changes can have on the end result. (SR)

  14. Synthesis and application of dual core Schiff base metal manganese like enzyme complex%双核希夫碱金属锰仿酶配合物的合成及其应用

    Institute of Scientific and Technical Information of China (English)

    张海希; 周向东

    2016-01-01

    以水杨醛、浓硫酸、碳酸钠与1,3-二氨基-2-丙醇为原料,采用2步法合成工艺制得双水杨醛磺酸钠缩二氨基-2-丙醇配体,再将配体与金属盐乙酸锰进行配位得到双核希夫碱金属锰配合物,红外光谱和能量色散X-射线能谱表征了配合物。将配合物应用于棉织物的低温漂白,探讨了配合物浓度、pH和乙酰胺用量对漂白效果的影响。结果表明,在配合物浓度为8μmol/L、pH为10.5~11.0、乙酰胺用量为8~9 g/L的条件下,处理后的棉织物白度达77.7%,毛细管效应达8.7 cm/30 min。%Dual core Schiff base metal manganese complex was synthesized by two step process. Firstly, double salicylic aldehyde condensation diamino-2-hydroxypropane ligand was synthesized by salicylaldehyde, concentrated sulfuric acid, sodium carbonate and 1,3-diamino-2-hydroxypropane. Then, Schiff base metal manganese complex was obtained through the coordination of ligand and manganese acetate. The complex was characterization by infrared and EDS. The complex was applied in low temperature bleaching of cotton fabric and the effects of complex concentration, pH, acetamide dosage on the bleaching were discussed. The results showed that when the cotton fabric treated with 8 μmol/L Schiff base complex, 8~9 g/L acetamide, pH=10.5~11.0, the whiteness of treated cotton fabric reached 77.7%, capil ary effect reached 8.7 cm/30 min.

  15. Surgical scar revision: An overview

    Directory of Open Access Journals (Sweden)

    Shilpa Garg

    2014-01-01

    Full Text Available Scar formation is an inevitable consequence of wound healing from either a traumatic or a surgical intervention. The aesthetic appearance of a scar is the most important criteria to judge the surgical outcome. An understanding of the anatomy and wound healing along with experience, meticulous planning and technique can reduce complications and improve the surgical outcome. Scar revision does not erase a scar but helps to make it less noticeable and more acceptable. Both surgical and non-surgical techniques, used either alone or in combination can be used for revising a scar. In planning a scar revision surgeon should decide on when to act and the type of technique to use for scar revision to get an aesthetically pleasing outcome. This review article provides overview of methods applied for facial scar revision. This predominantly covers surgical methods.

  16. 76 FR 72984 - Revised Application for a License To Export High-Enriched Uranium

    Science.gov (United States)

    2011-11-28

    ... COMMISSION Revised Application for a License To Export High-Enriched Uranium The application for a license to export high-enriched Uranium has been revised as noted below. Notice of this application was previously... kilograms To fabricate fuel France. Security Complex; October 18, Uranium (93.35%). uranium (174.0...

  17. 核壳型聚氨酯-聚丙烯酸酯复合乳液的合成及流变性能研究%Synthesis of core-shell polyurethane-polyacrylate complex emulsion and its rheological properties

    Institute of Scientific and Technical Information of China (English)

    张敬芳; 李雪峰; 王夏琴

    2012-01-01

    以水性聚氨酯(PU)乳液为种子,甲基丙烯酸甲酯(MMA)、苯乙烯(St)、丙烯酸丁酯(BA)、丙烯酸(AA)等为核层单体,采用半连续种子乳液聚合工艺,合成了具有核壳结构的水性聚氨酯-聚丙烯酸酯(PUA)复合乳液.通过透射电镜(TEM)、差示扫描量热分析(DSC)、傅里叶红外光谱(FTIR)、粒径分析等方法对乳液及其所成乳胶膜的结构和性能进行了测定.另外,还对PUA复合乳液及以其作为粘合剂所制成的数码喷墨印花墨水(乳液墨水)进行了流变性能研究,结果表明:PUA复合乳液在高速率剪切条件下呈现牛顿流体的特性,粘性在乳液体系中占主导地位;乳液墨水也表现出相似的流变学性能,乳液粒子与颜料粒子之间存在一定的相互作用.%Core-shell waterborne polyurethane-polyacrylate complex emulsion was prepared by semi-continuous seed emulsion polymerization using waterborne polyurethane (PU) emulsion as seed, methyl meth-acrylate (MMA), styrene (St), butyl acrylate (BA), and acrylic acid (AA) as core layer monomers. The structure and properties of emulsion and its latex film were characterized by transmission electron microscopy (TEM), differential scanning calorimetry (DSC), Fourier transmission infrared (FTIR). The rheological properties of PUA complex emulsion and its utilization as the binder for pigment inks of digital inkjet printing were thoroughly investigated. The results showed that PUA complex emulsion exhibited Newtonian fluid behaviors under high viscosity shearing condition and viscous behaviors dominated in the emulsion system. The emulsion ink occurred similar rheological properties, and there was some interaction between the emulsion and pigment particles.

  18. Scaling Turbo Boost to a 1000 cores

    CERN Document Server

    S, Ananth Narayan; Fedorova, Alexandra

    2010-01-01

    The Intel Core i7 processor code named Nehalem provides a feature named Turbo Boost which opportunistically varies the frequencies of the processor's cores. The frequency of a core is determined by core temperature, the number of active cores, the estimated power consumption, the estimated current consumption, and operating system frequency scaling requests. For a chip multi-processor(CMP) that has a small number of physical cores and a small set of performance states, deciding the Turbo Boost frequency to use on a given core might not be difficult. However, we do not know the complexity of this decision making process in the context of a large number of cores, scaling to the 100s, as predicted by researchers in the field.

  19. Investigating the translation of Earth's inner core

    DEFF Research Database (Denmark)

    Day, Elizabeth A; Cormier, Vernon F; Geballe, Zachary M;

    2012-01-01

    The Earth’s inner core provides unique insights into processes that are occurring deep within our Earth today, as well as processes that occurred in the past. The seismic structure of the inner core is complex, and is dominated by anisotropic and isotropic differences between the Eastern...... and Western ‘hemispheres’ of the inner core. Recent geodynamical models suggest that this hemispherical dichotomy can be explained by a fast translation of the inner core. In these models one side of the inner core is freezing, while the other side is melting, leading to the development of different seismic...... properties on either side of the inner core. A simple translating model of the inner core, however, does not seem to easily explain all of the seismically observed features, including the innermost inner core; the observed sharp lateral gradient in seismic properties between the two hemispheres...

  20. Philippines revises power plans

    Energy Technology Data Exchange (ETDEWEB)

    Hayes, D.

    1988-02-01

    Following an unexpected surge in electricity demand the Philippines has revised its medium term power development programme. The sharp increase in electricity consumption follows three years of slack demand due to civil disturbances before the end of the Macros administration and the election of Corazon Aquino as President in 1986. Originally, the Aquino government's medium term power development plans called for about 500MW of generating capacity to be installed between 1986 and 1992. The three proposed plants were scheduled for commissioning in 1991 and 1992. However, a spurt in power demand growth during the past nine months has caused concern among industrialists that power shortages could occur by the end of the decade. So additional capacity will be installed to prevent an anticipated shortfall in electricity supplies by the early 1990s.

  1. Financial analysis of revision knee surgery based on NHS tariffs and hospital costs: does it pay to provide a revision service?

    Science.gov (United States)

    Kallala, R F; Vanhegan, I S; Ibrahim, M S; Sarmah, S; Haddad, F S

    2015-02-01

    Revision total knee arthroplasty (TKA) is a complex procedure which carries both a greater risk for patients and greater cost for the treating hospital than does a primary TKA. As well as the increased cost of peri-operative investigations, blood transfusions, surgical instrumentation, implants and operating time, there is a well-documented increased length of stay which accounts for most of the actual costs associated with surgery. We compared revision surgery for infection with revision for other causes (pain, instability, aseptic loosening and fracture). Complete clinical, demographic and economic data were obtained for 168 consecutive revision TKAs performed at a tertiary referral centre between 2005 and 2012. Revision surgery for infection was associated with a mean length of stay more than double that of aseptic cases (21.5 vs 9.5 days, p < 0.0001). The mean cost of a revision for infection was more than three times that of an aseptic revision (£30 011 (sd 4514) vs £9655 (sd 599.7), p < 0.0001). Current NHS tariffs do not fully reimburse the increased costs of providing a revision knee surgery service. Moreover, especially as greater costs are incurred for infected cases. These losses may adversely affect the provision of revision surgery in the NHS.

  2. Tuning the Solubility of Copper Complex in Atom Transfer Radical Self-Condensing Vinyl Polymerizations to Control Polymer Topology via One-Pot to the Synthesis of Hyperbranched Core Star Polymers

    Directory of Open Access Journals (Sweden)

    Zong-Cheng Chen

    2014-09-01

    Full Text Available In this paper, we propose a simple one-pot methodology for proceeding from atom transfer reaction-induced conventional free radical polymerization (AT-FRP to atom transfer self-condensing vinyl polymerization (AT-SCVP through manipulation of the catalyst phase homogeneity (i.e., CuBr/2,2'-bipyridine (CuBr/Bpy in a mixture of styrene (St, 4-vinyl benzyl chloride (VBC, and ethyl 2-bromoisobutyrate. Tests of the solubilities of CuBr/Bpy and CuBr2/Bpy under various conditions revealed that both temperature and solvent polarity were factors affecting the solubility of these copper complexes. Accordingly, we obtained different polymer topologies when performing AT-SCVP in different single solvents. We investigated two different strategies to control the polymer topology in one-pot: varying temperature and varying solvent polarity. In both cases, different fractions of branching revealed the efficacy of varying the polymer topology. To diversify the functionality of the peripheral space, we performed chain extensions of the resulting hyperbranched poly(St-co-VBC macroinitiator (name as: hbPSt MI with either St or tBA (tert-butyl acrylate. The resulting hyperbranched core star polymer had high molecular weights (hbPSt-g-PSt: Mn = 25,000, Đ = 1.77; hbPSt-g-PtBA: Mn = 27,000, Đ = 1.98; hydrolysis of the tert-butyl groups of the later provided a hyperbranched core star polymer featuring hydrophilic poly(acrylic acid segments.

  3. Complex Network Community Detection Based on Core Graph Incremental Clustering%基于核心图增量聚类的复杂网络划分算法

    Institute of Scientific and Technical Information of China (English)

    张新猛; 蒋盛益

    2013-01-01

    借鉴基于聚类的无监督入侵检测算法(Clustering-based method for the unsupervised intrusion detection,CBUID)聚类原理,提出一种基于核心图增量聚类的社区划分算法(Clustering-based method for community detection,CBCD).本文提出一种社区构建方法,给出节点与社区相似度的计算公式.首先,对由少量高度数节点组成的核心网络采用现有算法进行核心社区划分,然后,采用增量方式依据节点与社区相似度,将剩余节点划分到核心社区中.算法复杂度主要依赖于网络规模、边的数量及划分的社区个数,具有线性复杂度.通过在几个典型真实网络数据集上测试,所提算法能够有效地进行社区划分.%This paper references the principle of clustering in clustering-based method for the unsupervised intrusion detection algorithm (CBUID),and proposes a clustering-based method for community detection (CBCD).We propose a method of community summary building,and give the formula of the similarity between node and community.First,it detects communities on the core network composed of a small amount of high-degree core nodes,then partitions the remaining nodes into core community according to the similarity between the node and community incrementally.Its running time mainly depends on the network size,the number of edges and the number of communities,and our algorithm has essentially a linear time complexity.Applications on several common real networks demonstrate that this method is very effective at community detection of networks.

  4. A study on scar revision

    Directory of Open Access Journals (Sweden)

    Ashutosh Talwar

    2016-04-01

    Full Text Available Introduction: Scars are psychologically distressing for the patients and have an impact on the quality of life and self esteem of the patients. Scar revision is an aesthetic skill which is mastered by plastic surgeons and encroached now by dermatosurgeons. Scars on the face are aesthetically unacceptable and various techniques have been improvised for making a scar aesthetically acceptable. Various types of techniques are used for scar revision like W plasty, Z plasty and VY plasty. Aims: To see the efficacy of various scar revision techniques including Z plasty, VY plasty and W plasty in 30 patients with disfiguring scars. Methods: We selected twenty patients of disfiguring scars for the study. The scars from various causes including trauma and burns were included in our study. Various techniques of scar revision include Z plasty, W plasty and VY plasty were performed according to the type and site of scar. Results: Male: female was 1.5: 1. The scar revision surgery yielded excellent results with minimal complications including haematoma formation, secondary infection and delayed healing seen in 5% patients each. Regarding the efficacy of scar revision, excellent improvement was seen in 60% patients, moderate improvement was seen in 30% patients and mild improvement was seen in 10% patients. Conclusions: Dermatologists can employ a number of surgical scar revision techniques. While some are better suited to treat specific types of scars, they can be used in combination with each other or with adjunctive therapies to achieve optimal results.

  5. Core stability training for injury prevention.

    Science.gov (United States)

    Huxel Bliven, Kellie C; Anderson, Barton E

    2013-11-01

    Enhancing core stability through exercise is common to musculoskeletal injury prevention programs. Definitive evidence demonstrating an association between core instability and injury is lacking; however, multifaceted prevention programs including core stabilization exercises appear to be effective at reducing lower extremity injury rates. PUBMED WAS SEARCHED FOR EPIDEMIOLOGIC, BIOMECHANIC, AND CLINICAL STUDIES OF CORE STABILITY FOR INJURY PREVENTION (KEYWORDS: "core OR trunk" AND "training OR prevention OR exercise OR rehabilitation" AND "risk OR prevalence") published between January 1980 and October 2012. Articles with relevance to core stability risk factors, assessment, and training were reviewed. Relevant sources from articles were also retrieved and reviewed. Stabilizer, mobilizer, and load transfer core muscles assist in understanding injury risk, assessing core muscle function, and developing injury prevention programs. Moderate evidence of alterations in core muscle recruitment and injury risk exists. Assessment tools to identify deficits in volitional muscle contraction, isometric muscle endurance, stabilization, and movement patterns are available. Exercise programs to improve core stability should focus on muscle activation, neuromuscular control, static stabilization, and dynamic stability. Core stabilization relies on instantaneous integration among passive, active, and neural control subsystems. Core muscles are often categorized functionally on the basis of stabilizing or mobilizing roles. Neuromuscular control is critical in coordinating this complex system for dynamic stabilization. Comprehensive assessment and training require a multifaceted approach to address core muscle strength, endurance, and recruitment requirements for functional demands associated with daily activities, exercise, and sport.

  6. Distributed k-Core Decomposition

    CERN Document Server

    Montresor, Alberto; Miorandi, Daniele

    2011-01-01

    Among the novel metrics used to study the relative importance of nodes in complex networks, k-core decomposition has found a number of applications in areas as diverse as sociology, proteinomics, graph visualization, and distributed system analysis and design. This paper proposes new distributed algorithms for the computation of the k-core decomposition of a network, with the purpose of (i) enabling the run-time computation of k-cores in "live" distributed systems and (ii) allowing the decomposition, over a set of connected machines, of very large graphs, that cannot be hosted in a single machine. Lower bounds on the algorithms complexity are given, and an exhaustive experimental analysis on real-world graphs is provided.

  7. Dual-core antiresonant hollow core fibers.

    Science.gov (United States)

    Liu, Xuesong; Fan, Zhongwei; Shi, Zhaohui; Ma, Yunfeng; Yu, Jin; Zhang, Jing

    2016-07-25

    In this work, dual-core antiresonant hollow core fibers (AR-HCFs) are numerically demonstrated, based on our knowledge, for the first time. Two fiber structures are proposed. One is a composite of two single-core nested nodeless AR-HCFs, exhibiting low confinement loss and a circular mode profile in each core. The other has a relatively simple structure, with a whole elliptical outer jacket, presenting a uniform and wide transmission band. The modal couplings of the dual-core AR-HCFs rely on a unique mechanism that transfers power through the air. The core separation and the gap between the two cores influence the modal coupling strength. With proper designs, both of the dual-core fibers can have low phase birefringence and short modal coupling lengths of several centimeters.

  8. Circumcision revision in male children

    Directory of Open Access Journals (Sweden)

    Mohammed A. Al-Ghazo

    2006-08-01

    Full Text Available OBJECTIVE: To determine indications for circumcision revision and to identify the specialty of the person who performed unsatisfactory primary circumcision. MATERIALS AND METHODS: The authors reviewed medical records of 52 cases that underwent circumcision revision over a 6-year period (1998 to 2004. Sleeve surgical technique was used for revision in patients with redundant foreskin or concealed penis, penoplasty for partial or complete degloving of the penis and meatotomy for external meatal stenosis. The mean age of children was 32 months (range 6 months to 9 years. RESULTS: Most of unsatisfactory primary circumcisions (86.7% were performed by laymen. All patients who underwent circumcision revision had good to excellent cosmetic results. CONCLUSION: Primary circumcision performed by laymen carry a high complication rate and serious complications may occur. A period of training and direct supervision by physicians is required before allowing laymen to perform circumcision independently.

  9. 170 - 174_Yarube_revised

    African Journals Online (AJOL)

    User

    acquisition, organization, utilization, and revision of knowledge ... The test was originally developed for use in rats to overcome stress ..... insulin or insulin resistance is associated with memory ... cerebral atrophy and white matter changes.

  10. Formation of micelles with complex coacervate cores.

    NARCIS (Netherlands)

    Cohen Stuart, M.A.; Besseling, N.A.M.; Fokkink, R.G.

    1998-01-01

    Micelles are commonly regarded as colloidal structures spontaneously formed by amphiphilic molecules, that is, molecules consisting of two distinct parts of which one is soluble and the other is insoluble. This definition is too restrictive: other kinds of molecules can also form micelles. We report

  11. [Pressure sore revision surgery].

    Science.gov (United States)

    Dorsche, Karin Marion

    2010-02-22

    Pressure sores are a major problem for patients as well as society in general. Immobilised patients are especially at risk. This group of patients with pressure sores should be hospitalised to perform surgical revision of the wound and reconstruction using a flap. Such surgery demands extensive postoperative relief of the flap. The University Centre for Wound Healing at Odense University Hospital has tested the effects of a reduction of the formerly recommended relief period from three to two weeks. In this article we report results covering all patients who have undergone surgery and reconstruction of pressure sores during the period from 1st October 2001 to 1st November 2008. The results are divided into two periods: the period before and the period after the introduction of the reduced relief period. A total of 80 patients were included; 34 in the first period and 46 in the second period. We achieved a considerable reduction in median length of stay from 38 to 27 days with no increase in surgical or complication frequency. Furthermore, the share of fully healed remained unchanged. We believe that there is no risk in shortening the immobile postoperative relief phase following reconstruction of pressure wounds in immobilised patients.

  12. Revision du Genre Aseraggodes Kaup

    NARCIS (Netherlands)

    Chabanaud, P.

    1930-01-01

    Le présent travail comprend la définition du genre Aseraggodes Kaup et la revision, sous forme d'une clef dichotomique, de toutes les espèces qui le composent, revision établie d'après les types eux-mêmes de ces espèces. Ce genre Aseraggodes appartient à la famille des Soleidae et à la sousfamille

  13. A study on scar revision

    OpenAIRE

    Ashutosh Talwar; Neerja Puri

    2016-01-01

    Introduction: Scars are psychologically distressing for the patients and have an impact on the quality of life and self esteem of the patients. Scar revision is an aesthetic skill which is mastered by plastic surgeons and encroached now by dermatosurgeons. Scars on the face are aesthetically unacceptable and various techniques have been improvised for making a scar aesthetically acceptable. Various types of techniques are used for scar revision like W plasty, Z plasty and VY plasty. Aims:...

  14. Transforming a Core Curriculum--and Minimizing the Battle Scars

    Science.gov (United States)

    Dwyer, Patricia M.

    2017-01-01

    It is notoriously difficult to change a core curriculum. As credit hours and course requirements are revised, politics quickly come into play and turf battles arise to create obstacles. The author writes that, in her experience, there are two default approaches to curricular change. The first is simply to "tweak" an existing…

  15. Revised accident source terms for light-water reactors

    Energy Technology Data Exchange (ETDEWEB)

    Soffer, L. [Nuclear Regulatory Commission, Washington, DC (United States)

    1995-02-01

    This paper presents revised accident source terms for light-water reactors incorporating the severe accident research insights gained in this area over the last 15 years. Current LWR reactor accident source terms used for licensing date from 1962 and are contained in Regulatory Guides 1.3 and 1.4. These specify that 100% of the core inventory of noble gases and 25% of the iodine fission products are assumed to be instantaneously available for release from the containment. The chemical form of the iodine fission products is also assumed to be predominantly elemental iodine. These assumptions have strongly affected present nuclear air cleaning requirements by emphasizing rapid actuation of spray systems and filtration systems optimized to retain elemental iodine. A proposed revision of reactor accident source terms and some im implications for nuclear air cleaning requirements was presented at the 22nd DOE/NRC Nuclear Air Cleaning Conference. A draft report was issued by the NRC for comment in July 1992. Extensive comments were received, with the most significant comments involving (a) release fractions for both volatile and non-volatile species in the early in-vessel release phase, (b) gap release fractions of the noble gases, iodine and cesium, and (c) the timing and duration for the release phases. The final source term report is expected to be issued in late 1994. Although the revised source terms are intended primarily for future plants, current nuclear power plants may request use of revised accident source term insights as well in licensing. This paper emphasizes additional information obtained since the 22nd Conference, including studies on fission product removal mechanisms, results obtained from improved severe accident code calculations and resolution of major comments, and their impact upon the revised accident source terms. Revised accident source terms for both BWRS and PWRS are presented.

  16. Revision of infected knee arthroplasties in Denmark

    DEFF Research Database (Denmark)

    Lindberg-Larsen, Martin; Jørgensen, Christoffer C; Bagger, Jens;

    2016-01-01

    Background and purpose - The surgical treatment of periprosthetic knee infection is generally either a partial revision procedure (open debridement and exchange of the tibial insert) or a 2-stage exchange arthroplasty procedure. We describe the failure rates of these procedures on a nationwide...... prosthesis with a re-revision rate due to infection of 34%, as compared to 55% in revisions of a revision prosthesis (p = 0.05). The failure rate of the 2-stage revisions was 30%. Median time interval between stages was 84 (9-597) days. 117 (54%) of the 2-stage revisions were revisions of a primary...... prosthesis with a re-revision rate due to infection of 21%, as compared to 29% in revisions of a previously revised prosthesis (p = 0.1). Overall postoperative mortality was 0.6% in high-volume centers (> 30 procedures within 2 years) as opposed to 7% in the remaining centers (p = 0.003). Interpretation...

  17. Observations of exotic inner core waves

    NARCIS (Netherlands)

    Waszek, Lauren; Deuss, A.F.

    2015-01-01

    The seismic structure of Earth’s inner core is highly complex, displaying strong anisotropy and further regional variations. However, few seismic waves are sensitive to the inner core and fundamental questions regarding the origin of the observed seismic features remain unanswered. Thus, new

  18. Animal MRI Core

    Data.gov (United States)

    Federal Laboratory Consortium — The Animal Magnetic Resonance Imaging (MRI) Core develops and optimizes MRI methods for cardiovascular imaging of mice and rats. The Core provides imaging expertise,...

  19. Revised Anatomy of Stars

    CERN Document Server

    Dubin, M; Dubin, Maurice; Soberman, Robert K.

    1997-01-01

    Stars accrete near invisible hydrogen dominated agglomerates. This population, the `dark matter,' effects the nature of stars. Measurements show plasma streams impacting Earth, planets, Sun and stars. This mass-energy source contradicts nebula collapse model for stars. The visual derived model, to which later discoveries (e.g., fusion) were appended, is confounded and contradicted by new observations. Discovery of a quantity of beryllium 7 (53 day half-life) in the Earth's upper atmosphere, fusion produced, hence from the solar outer zone, proves core fusion wrong. Magnetically pinched plasmas from aggregates impact stars at hundreds of km/s, create impulsive conditions for nuclear explosions below the surface. Disks with planets aid cluster capture. Planets modulate the influx varying fusion, hence luminosity (e.g., solar cycle). This population, with no assumptions or ad hoc physics, explains mysterious phenomena, e.g., luminosity/wind variation, sunspots, high temperature corona, CMEs, etc. Standard explan...

  20. The updated ESTRO core curricula 2011 for clinicians, medical physicists and RTTs in radiotherapy/radiation oncology.

    NARCIS (Netherlands)

    Eriksen, J.G.; Beavis, A.W.; Coffey, M.A.; Leer, J.W.H.; Magrini, S.M.; Benstead, K.; Boelling, T.; Hjalm-Eriksson, M.; Kantor, G.; Maciejewski, B.; Mezeckis, M.; Oliveira, A.; Thirion, P.; Vitek, P.; Olsen, D.R.; Eudaldo, T.; Enghardt, W.; Francois, P.; Garibaldi, C.; Heijmen, B.; Josipovic, M.; Major, T.; Nikoletopoulos, S.; Rijnders, A.; Waligorski, M.; Wasilewska-Radwanska, M.; Mullaney, L.; Boejen, A.; Vaandering, A.; Vandevelde, G.; Verfaillie, C.; Potter, R.

    2012-01-01

    INTRODUCTION: In 2007 ESTRO proposed a revision and harmonisation of the core curricula for radiation oncologists, medical physicists and RTTs to encourage harmonised education programmes for the professional disciplines, to facilitate mobility between EU member states, to reflect the rapid

  1. Challenges Regarding IP Core Functional Reliability

    Science.gov (United States)

    Berg, Melanie D.; LaBel, Kenneth A.

    2017-01-01

    For many years, intellectual property (IP) cores have been incorporated into field programmable gate array (FPGA) and application specific integrated circuit (ASIC) design flows. However, the usage of large complex IP cores were limited within products that required a high level of reliability. This is no longer the case. IP core insertion has become mainstream including their use in highly reliable products. Due to limited visibility and control, challenges exist when using IP cores and subsequently compromise product reliability. We discuss challenges and suggest potential solutions to critical application IP insertion.

  2. Dense SDM (12-Core × 3-Mode) Transmission Over 527 km With 33.2-ns Mode-Dispersion Employing Low-Complexity Parallel MIMO Frequency-Domain Equalization

    DEFF Research Database (Denmark)

    Shibahara, Kohki; Lee, Doohwan; Kobayashi, Takayuki;

    2016-01-01

    as intercore crosstalk. Mode dependent loss/gain effect was also mitigated by employing both a ring-core FM erbium-doped fiber amplifier and a free-space optics type gain equalizer. By combining these advanced techniques together, we finally demonstrate 12-core × 3-mode dense SDM transmission over 527-km GI MC...

  3. Core competencies for pain management: results of an interprofessional consensus summit.

    Science.gov (United States)

    Fishman, Scott M; Young, Heather M; Lucas Arwood, Ellyn; Chou, Roger; Herr, Keela; Murinson, Beth B; Watt-Watson, Judy; Carr, Daniel B; Gordon, Debra B; Stevens, Bonnie J; Bakerjian, Debra; Ballantyne, Jane C; Courtenay, Molly; Djukic, Maja; Koebner, Ian J; Mongoven, Jennifer M; Paice, Judith A; Prasad, Ravi; Singh, Naileshni; Sluka, Kathleen A; St Marie, Barbara; Strassels, Scott A

    2013-07-01

    The objective of this project was to develop core competencies in pain assessment and management for prelicensure health professional education. Such core pain competencies common to all prelicensure health professionals have not been previously reported. An interprofessional executive committee led a consensus-building process to develop the core competencies. An in-depth literature review was conducted followed by engagement of an interprofessional Competency Advisory Committee to critique competencies through an iterative process. A 2-day summit was held so that consensus could be reached. The consensus-derived competencies were categorized within four domains: multidimensional nature of pain, pain assessment and measurement, management of pain, and context of pain management. These domains address the fundamental concepts and complexity of pain; how pain is observed and assessed; collaborative approaches to treatment options; and application of competencies across the life span in the context of various settings, populations, and care team models. A set of values and guiding principles are embedded within each domain. These competencies can serve as a foundation for developing, defining, and revising curricula and as a resource for the creation of learning activities across health professions designed to advance care that effectively responds to pain. Wiley Periodicals, Inc.

  4. Conversion of core oxos to water molecules by 4e-/4H+ reductive dehydration of the Mn4O2(6+) core in the manganese-oxo cubane complex Mn4O4(Ph2PO2)6: a partial model for photosynthetic water binding and activation.

    Science.gov (United States)

    Ruettinger, W F; Dismukes, G C

    2000-03-06

    Reaction of the Mn4O4(6+) "cubane" core complex, Mn4O4L6 (1) (L = diphenylphosphinate, Ph2PO2-), with a hydrogen atom donor, phenothiazine (pzH), forms the dehydrated cluster Mn4O2L6 (2), which has lost two mu-oxo bridges by reduction to water (H2O). The formation of 2 was established by electrospray mass spectrometry, whereas FTIR spectroscopy confirmed the release of water molecules into solution during the reduction of 1. UV-vis and EPR spectroscopies established the stoichiometry and chemical form of the pzH product by showing the production of 4 equiv of the neutral pz radical. By contrast, the irreversible decomposition of 1 to individual Mn(II) ions occurs if the reduction is performed using electrons provided by various proton-lacking reductants, such as cobaltocene or electrochemical reduction. Thus, cubane 1 undergoes coupled four-electron/four-proton reduction with the release of two water molecules, a reaction formally analogous to the reverse sequence of the steps that occur during photosynthetic water oxidation leading to O2 evolution. 1H NMR of solutions of 2 reveal that all six of the phosphinate ligands exhibit paramagnetic broadening, due to coordination to Mn ions, and are magnetically equivalent. A symmetrical core structure is thus indicated. We hypothesize that this structure is produced by the dynamic averaging of phosphinato ligand coordination or exchange of mu-oxos between vacant mu-oxo sites. The paramagnetic 1H NMR of water molecules in solution shows that they are able to freely exchange with water molecules that are bound to the Mn ion(s) in 2, and this exchange can be inhibited by the addition of coordinating anions, such as chloride. Thus, 2 possesses open or labile coordination sites for water and anions, in contrast to solutions of 1, which reveal no evidence for water coordination. Complex 2 exhibits greater paramagnetism than that of 1, as seen by 1H NMR, and it possesses a broad (440 G wide) EPR absorption, centered at g = 2

  5. Advantages of iron core in a tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Bettis, E.S.; Ballou, J.K.; Becraft, W.R.; Peng, Y.K.M.; Watts, H.L.

    1977-01-01

    A quantitative comparison of the iron core vs air core concepts was carried out on a preliminary basis by using a representative tokamak reactor design with the following self-consistent reference parameters. In the area of plasma engineering, poloidal field and MHD equilibrium considerations with an unsaturated iron core is discussed. The question of proper poloidal field coils to maintain D-shaped plasmas of relatively high anti ..beta.. (7%) with a saturated iron core is also discussed. Estimates of the required iron core size, volt seconds, magnetic flux and its influence on force loading on the superconducting toroidal field coils are shown. Conceptual designs of the mechanical structure of an iron core device are presented. Favorable impacts on the OH power supply cost and complexity are indicated.

  6. Quantum interaction. Revised selected papers

    Energy Technology Data Exchange (ETDEWEB)

    Song, Dawei; Zhang, Peng; Wang, Lei [Aberdeen Univ. (United Kingdom). School of Computing; Melucci, Massimo [Padua Univ., Padova (Italy). Dept. of Information Engineering; Frommholz, Ingo [Bedfordshire Univ. (United Kingdom); Arafat, Sachi (eds.) [Glasgow Univ. (United Kingdom). School of Computing Science

    2011-07-01

    This book constitutes the thoroughly refereed post-conference proceedings of the 5th International Symposium on Quantum Interaction, QI 2011, held in Aberdeen, UK, in June 2011. The 26 revised full papers and 6 revised poster papers, presented together with 1 tutorial and 1 invited talk were carefully reviewed and selected from numerous submissions during two rounds of reviewing and improvement. The papers show the cross-disciplinary nature of quantum interaction covering topics such as computation, cognition, mechanics, social interaction, semantic space and information representation and retrieval. (orig.)

  7. Medical writing, revising and editing

    DEFF Research Database (Denmark)

    Pilegaard, Morten

    2006-01-01

    The globalization of science makes medical writing, editing and revision a rapidly growing field of linguistic study and practice. Medical science texts are written according to uniform, general guidelines and medical genres have become highly conventionalized in terms of structure and linguistic...... form. Medical editing often takes the form of peer review and mainly addresses issues of contents and overall validity. Medical revision incorporates the checking of the macrostructure and the microstructure of the text, its language and style and its suitability for the target reader or client...

  8. Medical writing, revising and editing

    DEFF Research Database (Denmark)

    Pilegaard, Morten

    2006-01-01

    The globalization of science makes medical writing, editing and revision a rapidly growing field of linguistic study and practice. Medical science texts are written according to uniform, general guidelines and medical genres have become highly conventionalized in terms of structure and linguistic...... form. Medical editing often takes the form of peer review and mainly addresses issues of contents and overall validity. Medical revision incorporates the checking of the macrostructure and the microstructure of the text, its language and style and its suitability for the target reader or client...

  9. Revising Nabokov Revising”

    Directory of Open Access Journals (Sweden)

    Marie Bouchet

    2010-10-01

    Full Text Available Nabokov revised his works as he translated them and, on another plane, canon revisionism has been having its backlash and provoked other refracting waves. The purpose of the conference was to advance Nabokov studies through the discussion of how our view of Nabokov’s standing and his works today should be revised, especially after the publication of The Original of Laura. However the conference was not confined to just this theme, since “revising” is a word rich with implications. To borrow s...

  10. One Health Core Competency Domains.

    Science.gov (United States)

    Frankson, Rebekah; Hueston, William; Christian, Kira; Olson, Debra; Lee, Mary; Valeri, Linda; Hyatt, Raymond; Annelli, Joseph; Rubin, Carol

    2016-01-01

    The emergence of complex global challenges at the convergence of human, animal, and environmental health has catalyzed a movement supporting "One Health" approaches. Despite recognition of the importance of One Health approaches to address these complex challenges, little effort has been directed at identifying the seminal knowledge, skills, and attitudes necessary for individuals to successfully contribute to One Health efforts. Between 2008 and 2011, three groups independently embarked on separate initiatives to identify core competencies for professionals involved with One Health approaches. Core competencies were considered critically important for guiding curriculum development and continuing professional education, as they describe the knowledge, skills, and attitudes required to be effective. A workshop was convened in 2012 to synthesize the various strands of work on One Health competencies. Despite having different mandates, participants, and approaches, all of these initiatives identified similar core competency domains: management; communication and informatics; values and ethics; leadership; teams and collaboration; roles and responsibilities; and systems thinking. These core competency domains have been used to develop new continuing professional education programs for One Health professionals and help university curricula prepare new graduates to be able to contribute more effectively to One Health approaches.

  11. One Health Core Competency Domains

    Science.gov (United States)

    Frankson, Rebekah; Hueston, William; Christian, Kira; Olson, Debra; Lee, Mary; Valeri, Linda; Hyatt, Raymond; Annelli, Joseph; Rubin, Carol

    2016-01-01

    The emergence of complex global challenges at the convergence of human, animal, and environmental health has catalyzed a movement supporting “One Health” approaches. Despite recognition of the importance of One Health approaches to address these complex challenges, little effort has been directed at identifying the seminal knowledge, skills, and attitudes necessary for individuals to successfully contribute to One Health efforts. Between 2008 and 2011, three groups independently embarked on separate initiatives to identify core competencies for professionals involved with One Health approaches. Core competencies were considered critically important for guiding curriculum development and continuing professional education, as they describe the knowledge, skills, and attitudes required to be effective. A workshop was convened in 2012 to synthesize the various strands of work on One Health competencies. Despite having different mandates, participants, and approaches, all of these initiatives identified similar core competency domains: management; communication and informatics; values and ethics; leadership; teams and collaboration; roles and responsibilities; and systems thinking. These core competency domains have been used to develop new continuing professional education programs for One Health professionals and help university curricula prepare new graduates to be able to contribute more effectively to One Health approaches. PMID:27679794

  12. One Health Core Competency Domains

    Directory of Open Access Journals (Sweden)

    Rebekah Frankson

    2016-09-01

    Full Text Available The emergence of complex global challenges at the convergence of human, animal, and environmental health has catalyzed a movement supporting ‘One Health’ approaches. Despite recognition of the importance of One Health approaches to address these complex challenges, little effort has been directed at identifying the seminal knowledge, skills and attitudes necessary for individuals to successfully contribute to One Health efforts. Between 2008 and 2011, three groups independently embarked on separate initiatives to identify core competencies for professionals involved with One Health approaches. Core competencies were considered critically important for guiding curriculum development and continuing professional education as they describe the knowledge, skills and attitudes required to be effective. A workshop was convened in 2012 to synthesize the various strands of work on One Health competencies. Despite having different mandates, participants, and approaches, all of these initiatives identified similar core competency domains: management; communication and informatics; values and ethics; leadership; teams and collaboration; roles and responsibilities; and systems thinking. These core competency domains have been used to develop new continuing professional education programs for One Health professionals and help university curricula prepare new graduates to be able to contribute more effectively to One Health approaches.

  13. k-core covers and the core

    NARCIS (Netherlands)

    Sanchez-Rodriguez, E.; Borm, Peter; Estevez-Fernandez, A.; Fiestras-Janeiro, G.; Mosquera, M.A.

    2015-01-01

    This paper extends the notion of individual minimal rights for a transferable utility game (TU-game) to coalitional minimal rights using minimal balanced families of a specific type, thus defining a corresponding minimal rights game. It is shown that the core of a TU-game coincides with the core of

  14. Academic Rigor: The Core of the Core

    Science.gov (United States)

    Brunner, Judy

    2013-01-01

    Some educators see the Common Core State Standards as reason for stress, most recognize the positive possibilities associated with them and are willing to make the professional commitment to implementing them so that academic rigor for all students will increase. But business leaders, parents, and the authors of the Common Core are not the only…

  15. k-core covers and the core

    NARCIS (Netherlands)

    Sanchez-Rodriguez, E.; Borm, Peter; Estevez-Fernandez, A.; Fiestras-Janeiro, G.; Mosquera, M.A.

    This paper extends the notion of individual minimal rights for a transferable utility game (TU-game) to coalitional minimal rights using minimal balanced families of a specific type, thus defining a corresponding minimal rights game. It is shown that the core of a TU-game coincides with the core of

  16. Diet History Questionnaire: Database Revision History

    Science.gov (United States)

    The following details all additions and revisions made to the DHQ nutrient and food database. This revision history is provided as a reference for investigators who may have performed analyses with a previous release of the database.

  17. Revised Human Health Risk Assessment on Chlorpyrifos

    Science.gov (United States)

    We have revised our human health risk assessment and drinking water exposure assessment for chlorpyrifos that supported our October 2015 proposal to revoke all food residue tolerances for chlorpyrifos. Learn about the revised analysis.

  18. Diet History Questionnaire: Database Revision History

    Science.gov (United States)

    The following details all additions and revisions made to the DHQ nutrient and food database. This revision history is provided as a reference for investigators who may have performed analyses with a previous release of the database.

  19. Revision of failed humeral head resurfacing arthroplasty

    Directory of Open Access Journals (Sweden)

    Philipp N Streubel

    2016-01-01

    Conclusion: Outcomes of revision of HHR arthroplasty in this cohort did not improve upon those reported for revision of stemmed humeral implants. A comparative study would be required to allow for definitive conclusions to be made.

  20. Soft tissue trauma and scar revision.

    Science.gov (United States)

    Mobley, Steven R; Sjogren, Phayvanh P

    2014-11-01

    Numerous techniques and treatments have been described for scar revision, with most studies focusing on the adult population. A comprehensive review of the literature reveals a paucity of references related specifically to scar revision in children. This review describes the available modalities in pediatric facial scar revision. The authors have integrated current practices in soft tissue trauma and scar revision, including closure techniques and materials, topical therapy, steroid injection, cutaneous laser therapy, and tissue expanders.

  1. On-Line Core Thermal-Hydraulic Model Improvement

    Energy Technology Data Exchange (ETDEWEB)

    In, Wang Kee; Chun, Tae Hyun; Oh, Dong Seok; Shin, Chang Hwan; Hwang, Dae Hyun; Seo, Kyung Won

    2007-02-15

    The objective of this project is to implement a fast-running 4-channel based code CETOP-D in an advanced reactor core protection calculator system(RCOPS). The part required for the on-line calculation of DNBR were extracted from the source of the CETOP-D code based on analysis of the CETOP-D code. The CETOP-D code was revised to maintain the input and output variables which are the same as in CPC DNBR module. Since the DNBR module performs a complex calculation, it is divided into sub-modules per major calculation step. The functional design requirements for the DNBR module is documented and the values of the database(DB) constants were decided. This project also developed a Fortran module(BEST) of the RCOPS Fortran Simulator and a computer code RCOPS-SDNBR to independently calculate DNBR. A test was also conducted to verify the functional design and DB of thermal-hydraulic model which is necessary to calculate the DNBR on-line in RCOPS. The DNBR margin is expected to increase by 2%-3% once the CETOP-D code is used to calculate the RCOPS DNBR. It should be noted that the final DNBR margin improvement could be determined in the future based on overall uncertainty analysis of the RCOPS.

  2. Revised hypothesis and future perspectives

    DEFF Research Database (Denmark)

    Norsk, P; Drummer, C; Christensen, N J

    2001-01-01

    Results from space have been unexpected and not predictable from the results of ground-based simulations. Therefore, the concept of how weightlessness and gravity modulates the regulation of body fluids must be revised and a new simulation model developed. The main questions to ask in the future ...

  3. Air Pollution Primer. Revised Education.

    Science.gov (United States)

    Corman, Rena

    This revised and updated book is written to inform the citizens on the nature, causes, and effects of air pollution. It is written in terms familiar to the layman with the purpose of providing knowledge and motivation to spur community action on clean air policies. Numerous charts and drawings are provided to support discussion of air pollution…

  4. Revised Safety Code A2

    CERN Multimedia

    SC Secretariat

    2005-01-01

    Please note that the revised Safety Code A2 (Code A2 rev.) entitled "REPORTING OF ACCIDENTS AND NEAR MISSES" is available on the web at the following url: https://edms.cern.ch/document/335502/LAST_RELEASED Paper copies can also be obtained from the SC Unit Secretariat, e-mail: sc.secretariat@cern.ch SC Secretariat

  5. Revised Accounting for Business Combinations

    Science.gov (United States)

    Wilson, Arlette C.; Key, Kimberly

    2008-01-01

    The Financial Accounting Standards Board (FASB) has recently issued Statement of Financial Accounting Standards No. 141 (Revised 2007) Business Combinations. The object of this Statement is to improve the relevance, representational faithfulness, and comparability of reported information about a business combination and its effects. This Statement…

  6. Ethical considerations in revision rhinoplasty.

    Science.gov (United States)

    Wayne, Ivan

    2012-08-01

    The problems that arise when reviewing another surgeon's work, the financial aspects of revision surgery, and the controversies that present in marketing and advertising will be explored. The technological advances of computer imaging and the Internet have introduced new problems that require our additional consideration.

  7. Revision of Drymophloeus (Areceacea: Arecoideae)

    NARCIS (Netherlands)

    Zona, Scott

    1999-01-01

    A revision of the genus Drymophloeus (Arecaceae: Arecoideae: Ptychospermatinae) recognizes seven species, distributed from the Maluku Islands of Indonesia to Western Samoa. The history of the genus is reviewed. A key, species descriptions, a complete list of synonymy, a list of specimens examined, i

  8. A revision of Ichnocarpus (Apocynaceae)

    NARCIS (Netherlands)

    Middleton, D.J.

    1994-01-01

    The genus Ichnocarpus is revised. A total of 12 species are recognised, of which one new species is described. Three new combinations in Ichnocarpus and one in Anodendron are made. Micrechites and Lamechites are treated as synonyms of Ichnocarpus. Nomina nuda and species exclusae have been given as

  9. Curriculum revision and ICT integration

    OpenAIRE

    Atanasova-Pacemska, Tatjana

    2006-01-01

    In this paper we will present the possibilities for revision and development of the curriculum of "Math Teaching Methods" related to Child-centered methodology and ICT integration. This paper is a result of the projects: "Teacher, Technology and young learners" and "Child-centered methodology" supported by USAID and World Learning.

  10. Complex analysis

    CERN Document Server

    Freitag, Eberhard

    2005-01-01

    The guiding principle of this presentation of ``Classical Complex Analysis'' is to proceed as quickly as possible to the central results while using a small number of notions and concepts from other fields. Thus the prerequisites for understanding this book are minimal; only elementary facts of calculus and algebra are required. The first four chapters cover the essential core of complex analysis: - differentiation in C (including elementary facts about conformal mappings) - integration in C (including complex line integrals, Cauchy's Integral Theorem, and the Integral Formulas) - sequences and series of analytic functions, (isolated) singularities, Laurent series, calculus of residues - construction of analytic functions: the gamma function, Weierstrass' Factorization Theorem, Mittag-Leffler Partial Fraction Decomposition, and -as a particular highlight- the Riemann Mapping Theorem, which characterizes the simply connected domains in C. Further topics included are: - the theory of elliptic functions based on...

  11. A multistage crucible of revision and approval shapes IPCC policymaker summaries.

    Science.gov (United States)

    Mach, Katharine J; Freeman, Patrick T; Mastrandrea, Michael D; Field, Christopher B

    2016-08-01

    Intergovernmental Panel on Climate Change (IPCC) member governments approve each report's summary for policymakers (SPM) by consensus, discussing and agreeing on each sentence in a plenary session with scientist authors. A defining feature of IPCC assessment, the governmental approval process builds joint ownership of current knowledge by scientists and governments. The resulting SPM revisions have been extensively discussed in anecdotes, interviews, and perspectives, but they have not been comprehensively analyzed. We provide an in-depth evaluation of IPCC SPM revisions, establishing an evidential basis for understanding their nature. Revisions associated with governmental review and approval generally expand SPMs, with SPM text growing by 17 to 53% across recent assessment reports. Cases of high political sensitivity and failure to reach consensus are notable exceptions, resulting in SPM contractions. In contrast to recent claims, we find that IPCC SPMs are as readable, for multiple metrics of reading ease, as other professionally edited assessment summaries. Across reading-ease metrics, some SPMs become more readable through governmental review and approval, whereas others do not. In an SPM examined through the entire revision process, most revisions associated with governmental review and approval occurred before the start of the government-approval plenary session. These author revisions emphasize clarity, scientific rigor, and explanation. In contrast, the subsequent plenary revisions place greater emphasis especially on policy relevance, comprehensiveness of examples, and nuances of expert judgment. Overall, the value added by the IPCC process emerges in a multistage crucible of revision and approval, as individuals together navigate complex science-policy terrain.

  12. REVISION MASTOIDECTOMY AND ITS GOAL

    Directory of Open Access Journals (Sweden)

    Sampath Kumar Singh Katewad

    2016-06-01

    Full Text Available BACKGROUND The main aims in doing surgery for chronic otitis media are: 1. Complete clearance of progressive disease from its site and form dry and well-epithelialised cavity. 2. Prevention of recurrent and residual cholesteatoma achieved by modifying the anatomy of tympanomastoid compartments. 3. Hearing improvement by reconstructing the ossicles and tympanic membrane. The main indication for revision surgery is failure to achieve above said aims by previous surgeon. The aim of our study was to identify the causes of recurrent disease and the factors that helps in chronic otitis media surgery to minimise the revisions & report the results of revision mastoidectomy. MATERIALS AND METHODS In this study, thirty patients are selected and operated for revision mastoidectomy surgery at our institute during the period from May 2013 – Dec 2015. These cases were analysed retrospectively, patients who had discharging ear with the history of previous intact canal wall and canal down mastoidectomy surgeries were selected for this study. OBSERVATION AND RESULTS In this study, the common age group of patients who underwent revision surgery - 8-46 yrs. (mean 19 yrs.. Majority of patients are female, 16 cases (53.33%; and males 14 cases (46.66%. Revision mastoidectomies were applied to 12 cases (40% of previous canal wall up mastoidectomies and 18 cases (60% of prior canal down mastoidectomies. 60% of cases had residual/recurrent cholesteatoma which was the most common finding seen. While in 33.3% cases patient had only chronic granulations. The most frequent site of cholesteatoma was mastoid antrum/mastoid cavity seen in 73% followed by attic 42.3% and mesotympanum in 40% of cases. The common failure in primary surgery was inadequate clearance of diseased mastoid air cells - 48%, high facial bridge - 48%, stenotic meatoplasty - 40%, incomplete removal of buttress - 30%. Tympanic membrane perforation - 6.66% of cases with poor architecture of mastoidectomy

  13. CUSTOMIZED ACETABULAR COMPONENTS IN REVISION HIP ARTHROPLASTY

    Directory of Open Access Journals (Sweden)

    G. M. Kavalersky

    2016-01-01

    Full Text Available In recent years, there is a trend of increasing demand for revision hip arthroplasty. Among these patients there are many with complex acetabular defects, including patients with pelvic discontinuity. To ensure stability for revised acetabular components in such cases becomes a challenging or unachievable task. Such defects give indications for printing customized tri-flange acetabular component. The authors analysed own experience of creating and applying custom made acetabular components in 3 patients with complex acetabular defects. Material and methods. Among the patients there were 2 women and 1 man. Average age was 60,3±19,4 years (38 to 78 years. Two patients had III B defects with pelvic discontinuity and one patient had III A defect by Paprosky classification. As the first step, the authors in collaboration with engineers printed a plaster full size pelvic 3D model, as the second step a customized tri-flange acetabular component was designed and printed. Harris Hip Score was evaluated preoperatively and 3 months postoperatively. Results. Average follow-up period was 5,3±2,5 months (3 to 8 months. The authors observed no cases of implant loosening, dislocation or deep periprosthetic infection. Average Harris Hip Score before surgery was 27,13 and after surgery – 74,1 indicating a significant improvement in 3 months postoperatively. Conclusion. Indications for use of individual acetabular components in reported patients correspond to indications formulated by Berasi et al. The authors obtained encouraging early follow-up outcomes that correspond to data of other authors. In one patient certain difficulties were reported due to insufficient pelvic distraction. Component’s flanges prevented achieving adequate pelvic distraction. Nevertheless, good primary stability was achieved. Modern software and 3D metal printers can significantly reduce the production cost of customized acetabular components. Application of this technology can be

  14. Revising Psychiatry's Charter Document: "DSM-IV."

    Science.gov (United States)

    McCarthy, Lucille Parkinson; Gerring, Joan Page

    1994-01-01

    Reports findings from a three-year study by a composition researcher and a psychiatrist of the revision of an important mental health book: "Diagnostic and Statistical Manual of Mental Disorders." Examines the revision using three methodologies. Concludes that the revision functions to achieve certain social and political effects. (HB)

  15. Humeral windows in revision total elbow arthroplasty

    Science.gov (United States)

    Salama, Amir; Stanley, David

    2016-01-01

    The use of cortical windows for revision elbow arthroplasty has not previously been widely reported. Their use aids safe revision of a well fixed humeral prosthesis and can be used in the setting of dislocation, periprosthetic fracture or aseptic loosening of the ulnar component. We describe our technique and results of cortical windows in the distal humerus for revision elbow arthroplasty surgery. PMID:27583011

  16. A taxonomic revision of the genus Podocarpus

    NARCIS (Netherlands)

    Laubenfels, de D.J.

    1985-01-01

    In connection with the forthcoming revision of the Coniferae for the Flora Malesiana, the author thought it necessary to revise the genus Podocarpus. Although this genus has a substantial representation in Malesia (30 species), the revision is too involved to be appropriate with the Flora Malesiana

  17. Crystal Structure of the Human Cytomegalovirus pUL50-pUL53 Core Nuclear Egress Complex Provides Insight into a Unique Assembly Scaffold for Virus-Host Protein Interactions.

    Science.gov (United States)

    Walzer, Sascha A; Egerer-Sieber, Claudia; Sticht, Heinrich; Sevvana, Madhumati; Hohl, Katharina; Milbradt, Jens; Muller, Yves A; Marschall, Manfred

    2015-11-13

    Nuclear replication of cytomegalovirus relies on elaborate mechanisms of nucleocytoplasmic egress of viral particles. Thus, the role of two essential and conserved viral nuclear egress proteins, pUL50 and pUL53, is pivotal. pUL50 and pUL53 heterodimerize and form a core nuclear egress complex (NEC), which is anchored to the inner nuclear membrane and provides a scaffold for the assembly of a multimeric viral-cellular NEC. Here, we report the crystal structure of the pUL50-pUL53 heterodimer (amino acids 1-175 and 50-292, respectively) at 2.44 Å resolution. Both proteins adopt a globular fold with mixed α and β secondary structure elements. pUL53-specific features include a zinc-binding site and a hook-like N-terminal extension, the latter representing a hallmark element of the pUL50-pUL53 interaction. The hook-like extension (amino acids 59-87) embraces pUL50 and contributes 1510 Å(2) to the total interface area (1880 Å(2)). The pUL50 structure overall resembles the recently published NMR structure of the murine cytomegalovirus homolog pM50 but reveals a considerable repositioning of the very C-terminal α-helix of pUL50 upon pUL53 binding. pUL53 shows structural resemblance with the GHKL domain of bacterial sensory histidine kinases. A close examination of the crystal structure indicates partial assembly of pUL50-pUL53 heterodimers to hexameric ring-like structures possibly providing additional scaffolding opportunities for NEC. In combination, the structural information on pUL50-pUL53 considerably improves our understanding of the mechanism of HCMV nuclear egress. It may also accelerate the validation of the NEC as a unique target for developing a novel type of antiviral drug and improved options of broad-spectrum antiherpesviral therapy.

  18. Novel point mutations and mutational complexes in the enhancer II, core promoter and precore regions of hepatitis B virus genotype D1 associated with hepatocellular carcinoma in Saudi Arabia.

    Science.gov (United States)

    Khan, Anis; Al Balwi, Mohammed A; Tanaka, Yasuhito; Hajeer, Ali; Sanai, Faisal M; Al Abdulkarim, Ibrahim; Al Ayyar, Latifah; Badri, Motasim; Saudi, Dib; Tamimi, Waleed; Mizokami, Masashi; Al Knawy, Bandar

    2013-12-15

    In this study, a cohort of 182 patients [55 hepatocellular carcinoma (HCC) and 127 non-HCC] infected with hepatitis B virus (HBV) in Saudi Arabia was investigated to study the relationship between sequence variation in the enhancer II (EnhII), basal core promoter (BCP) and precore regions of HBV genotype D (HBV/D) and the risk of HCC. HBV genotypes were determined by sequencing analysis and/or enzyme-linked immunosorbent assay. Variations in the EnhII, BCP and precore regions were compared between 107 non-HCC and 45 HCC patients infected with HBV/D, followed by age-matched analysis of 40 cases versus equal number of controls. Age and male gender were significantly associated with HCC (p = 0.0001 and p = 0.03, respectively). Serological markers such as aspartate aminotransferase, albumin and anti-HBe were significantly associated with HCC (p = 0.0001 for all), whereas HBeAg positivity was associated with non-HCC (p = 0.0001). The most prevalent HBV genotype was HBV/D (94%), followed by HBV/E (4%), HBV/A (1.6%) and HBV/C (0.5%). For HBV/D1, genomic mutations associated with HCC were T1673/G1679, G1727, C1741, C1761, A1757/T1764/G1766, T1773, T1773/G1775 and C1909. Age- and gender-adjusted stepwise logistic regression analysis indicated that mutations G1727 [odds ratio (OR) = 18.3; 95% confidence interval (CI) = 2.8-118.4; p = 0.002], A1757/T1764/G1766 (OR = 4.7; 95% CI = 1.3-17.2; p = 0.01) and T1773 (OR = 14.06; 95% CI = 2.3-84.8; p = 0.004) are independent predictors of HCC development. These results implicate novel individual and combination patterns of mutations in the X/precore region of HBV/D1 as predictors of HCC. Risk stratification based on these mutation complexes would be useful in determining high-risk patients and improving diagnostic and treatment strategies for HBV/D1.

  19. Growth outside the core.

    Science.gov (United States)

    Zook, Chris; Allen, James

    2003-12-01

    Growth in an adjacent market is tougher than it looks; three-quarters of the time, the effort fails. But companies can change those odds dramatically. Results from a five-year study of corporate growth conducted by Bain & Company reveal that adjacency expansion succeeds only when built around strong core businesses that have the potential to become market leaders. And the best place to look for adjacency opportunities is inside a company's strongest customers. The study also found that the most successful companies were able to consistently, profitably outgrow their rivals by developing a formula for pushing out the boundaries of their core businesses in predictable, repeatable ways. Companies use their repeatability formulas to expand into any number of adjacencies. Some companies make repeated geographic moves, as Vodafone has done in expanding from one geographic market to another over the past 13 years, building revenues from $1 billion in 1990 to $48 billion in 2003. Others apply a superior business model to new segments. Dell, for example, has repeatedly adapted its direct-to-customer model to new customer segments and new product categories. In other cases, companies develop hybrid approaches. Nike executed a series of different types of adjacency moves: it expanded into adjacent customer segments, introduced new products, developed new distribution channels, and then moved into adjacent geographic markets. The successful repeaters in the study had two common characteristics. First, they were extraordinarily disciplined, applying rigorous screens before they made an adjacency move. This discipline paid off in the form of learning curve benefits, increased speed, and lower complexity. And second, in almost all cases, they developed their repeatable formulas by studying their customers and their customers' economics very, very carefully.

  20. Athletes and sports teams as complex adaptive system: A review of implications for learning design. [Atletas y equipos deportivos como sistemas adaptativos complejos: Una revision de las Implicaciones para el diseño del aprendizaje].

    Directory of Open Access Journals (Sweden)

    Keith Davids

    2015-01-01

    Full Text Available Ecological dynamics is a systems-oriented theoretical framework which conceptualises sport performers as complex adaptive systems. It seeks to understand the adaptive relations that emerge during coordination of interactions between each performer and a specific performance environment. This approach has identified the individual-environment relationship as the relevant scale of analysis for explaining how processes of perception, cognition and action underpin expert performance in sport. This theoretical overview elucidates key ideas from previous work identifying functional characteristics of complex adaptive systems, such as co-adaptation, emergent coordination tendencies and capacity to utilise affordances, which underlie performance and learning in team and individual sports. The review of research focuses on how key principles of ecological dynamics inform our understanding of learning and transfer, and their impact on practice task design in sport development programmes. To support this analysis, data from research on performance of elite and developmental athletes in individual and team sports are presented to highlight important principles of learning design from an ecological dynamics perspective. Resumen La dinámica ecológica es un marco teórico orientado a los sistemas que conceptualiza a los athletas como sistemas complejos adaptativos. Con el objeto de comprender las relaciones adaptativas que surgen durante la coordinación de las interacciones entre cada uno de los athletas y un medio/ambiente de actuación específico. Este enfoque ha identificado la relación individuo-ambiente como la escala de análisis relevante para explicar cómo los procesos de la percepción, la cognición y la acción respaldan el rendimiento de expertos en el deporte. Este documento de posición teórica aclara características funcionales esenciales de los sistemas adaptativos complejos, tales como co-adaptación, tendencias emergentes de

  1. Effects of Task Complexity on L2 Writing Behaviors and Linguistic Complexity

    Science.gov (United States)

    Révész, Andrea; Kourtali, Nektaria-Efstathia; Mazgutova, Diana

    2017-01-01

    This study investigated whether task complexity influences second language (L2) writers' fluency, pausing, and revision behaviors and the cognitive processes underlying these behaviors; whether task complexity affects linguistic complexity of written output; and whether relationships between writing behaviors and linguistic complexity are…

  2. Effects of Task Complexity on L2 Writing Behaviors and Linguistic Complexity

    Science.gov (United States)

    Révész, Andrea; Kourtali, Nektaria-Efstathia; Mazgutova, Diana

    2017-01-01

    This study investigated whether task complexity influences second language (L2) writers' fluency, pausing, and revision behaviors and the cognitive processes underlying these behaviors; whether task complexity affects linguistic complexity of written output; and whether relationships between writing behaviors and linguistic complexity are…

  3. Comodules over semiperfect corings

    CERN Document Server

    Caenepeel, S

    2011-01-01

    We discuss when the Rat functor associated to a coring satisfying the left $\\alpha$-condition is exact. We study the category of comodules over a semiperfect coring. We characterize semiperfect corings over artinian rings and over qF-rings.

  4. Coring Sample Acquisition Tool

    Science.gov (United States)

    Haddad, Nicolas E.; Murray, Saben D.; Walkemeyer, Phillip E.; Badescu, Mircea; Sherrit, Stewart; Bao, Xiaoqi; Kriechbaum, Kristopher L.; Richardson, Megan; Klein, Kerry J.

    2012-01-01

    A sample acquisition tool (SAT) has been developed that can be used autonomously to sample drill and capture rock cores. The tool is designed to accommodate core transfer using a sample tube to the IMSAH (integrated Mars sample acquisition and handling) SHEC (sample handling, encapsulation, and containerization) without ever touching the pristine core sample in the transfer process.

  5. A Discussion of the Various Groups of Readers in the Fairytale Project, with a Detailed Description of the "Core-Groups" in Denmark and Turkey. Fairytale: An Interdisciplinary Turco-Danish Study of the Collective v. the Individual Nature of the Response to Literature. Report No. 10. Revised.

    Science.gov (United States)

    Dollerup, Cay; And Others

    Prepared as part of the interdisciplinary Turko-Danish Fairytale Project, this paper contains descriptions of the core group of readers (600 18-year-old students) and the special groups of readers (each composed of approximately 50 subjects) who participated in the investigation of the collective versus the individual nature of the reader response…

  6. Revision Gore-Tex medialization laryngoplasty.

    Science.gov (United States)

    Cohen, Jacob T; Bates, Dwight D; Postma, Gregory N

    2004-09-01

    To evaluate the indications, results, and safety of revision Gore-Tex medialization laryngoplasty (GML). A retrospective chart review of 156 patients that underwent GML procedures between the years 1998-2002. Study population consisted of those patients who required revision surgery for any reason. Sixteen patients required 22 revision procedures. Indications for revision were divided into 2 groups, complications and glottal closure problems. Complications included extruded or displaced implants (n = 4). The most common glottal closure problem was undercorrection (n = 9). Others included anterior overcorrection (n = 1) and persistent posterior glottal gap (n = 2). Revision procedures included GML (n = 9), injection augmentation (n = 9), endoscopic implant removal (n = 2), and arytenoid adduction (n = 2). In patients with glottal closure problems, the GCI improved in all 10 and the voice rating scale improved in 9. Reasons for revision of GML are variable, the most common being undercorrection. A variety of safe, effective revision techniques are available with a high success rate.

  7. Core formation in silicate bodies

    Science.gov (United States)

    Nimmo, F.; O'Brien, D. P.; Kleine, T.

    2008-12-01

    -equilibration is also suggested by mantle siderophile abundances [13], though simple partitioning models do not capture the likely complex P,T evolution during successive giant impacts. The timescale of Martian core formation is currently uncertain (0-10 My) [14], though it is clear that Martian core formation ended before that of the Earth. [1] Stevenson, in Origin of the Earth, 1990. [2] Groebner and Kohlstedt, EPSL 2006. [3] Rubie et al., Treatise Geophys. 2007. [4] Kleine et al., GCA submitted. [5] Weiss et al., LPSC 39, 2008. [6] Keil and Wilson, EPSL 1993 [7] Wanke and Dreibus, PTRSL, 1984. [8] Agnor et al. Icarus 1999 [9] Canup and Asphaug, Nature 2001 [10] Nimmo and Agnor, EPSL 2006. [11] Rubie et al., EPSL 2003 [12] O'Brien et al, Icarus 2006 [13] Righter, AREPS 2003. [14] Nimmo and Kleine, Icarus 2007.

  8. Banded transformer cores

    Science.gov (United States)

    Mclyman, C. W. T. (Inventor)

    1974-01-01

    A banded transformer core formed by positioning a pair of mated, similar core halves on a supporting pedestal. The core halves are encircled with a strap, selectively applying tension whereby a compressive force is applied to the core edge for reducing the innate air gap. A dc magnetic field is employed in supporting the core halves during initial phases of the banding operation, while an ac magnetic field subsequently is employed for detecting dimension changes occurring in the air gaps as tension is applied to the strap.

  9. Belief Revision and Argumentation Theory

    Science.gov (United States)

    Falappa, Marcelo Alejandro; Kern-Isberner, Gabriele; Simari, Guillermo Ricardo

    Belief revision is the process of changing beliefs to adapt the epistemic state of an agent to a new piece of information. The logical formalization of belief revision is a topic of research in philosophy, logic, and in computer science, in areas such as databases or artificial intelligence. On the other hand, argumentation is concerned primarily with the evaluation of claims based on premises in order to reach conclusions. Both provide basic and substantial techniques for the art of reasoning, as it is performed by human beings in everyday life situations and which goes far beyond logical deduction. Reasoning, in this sense, makes possible to deal successfully with problems in uncertain, dynamic environments and has been promoting the development of human societies.

  10. Idiopathic interstitial pneumonias: Classification revision

    Directory of Open Access Journals (Sweden)

    Demosthenes Bouros MD, PhD, FCCP

    2010-01-01

    Full Text Available The American Thoracic Society (ATS, the European Respiratory Society (ERS and the Japan Respiratory Society (JRS are planning a revision of the 2002 ATS/ERS International Multidisciplinary Classification of Idiopathic Interstitial Pneumonias (IIPs1. In two years’ time it will be 10 years since its publication and with a view to publishing the revision after 10 years (i.e., in 2012, a steering committee has been established, which met in New Orleans during ATS congress in May 2010 and more recently in Barcelona during the ERS congress (Photo. The committee will meet again during the ATS and the ERS congresses that will be held in the next two years, with an additional meeting in Modena, Italy, in Αpril 2011.

  11. Revising Academic Library Governance Handbooks

    Directory of Open Access Journals (Sweden)

    Jen Stevens

    2015-07-01

    Full Text Available Regardless of our status (tenure track, non-tenure track, staff, and/or union, academic librarians at colleges and universities may use a handbook or similar document as a framework for self-governance. These handbooks typically cover rank descriptions, promotion requirements, and grievance rights, among other topics. Unlike employee handbooks used in the corporate world, these documents may be written and maintained by academic librarians themselves1. In 2010, a group of academic librarians at George Mason University was charged with revising our Librarians’ Handbook. Given the dearth of literature about academic librarians’ handbooks and their revision, we anticipate our library colleagues in similar situations will benefit from our experience and recommendations.

  12. Clean Air Act. Revision 5

    Energy Technology Data Exchange (ETDEWEB)

    1994-02-15

    This Reference Book contains a current copy of the Clean Air Act, as amended, and those regulations that implement the statute and appear to be most relevant to DOE activities. The document is provided to DOE and contractor staff for informational purposes only and should not be interpreted as legal guidance. This Reference Book has been completely revised and is current through February 15, 1994.

  13. Complex dynamics

    CERN Document Server

    Carleson, Lennart

    1993-01-01

    Complex dynamics is today very much a focus of interest. Though several fine expository articles were available, by P. Blanchard and by M. Yu. Lyubich in particular, until recently there was no single source where students could find the material with proofs. For anyone in our position, gathering and organizing the material required a great deal of work going through preprints and papers and in some cases even finding a proof. We hope that the results of our efforts will be of help to others who plan to learn about complex dynamics and perhaps even lecture. Meanwhile books in the field a. re beginning to appear. The Stony Brook course notes of J. Milnor were particularly welcome and useful. Still we hope that our special emphasis on the analytic side will satisfy a need. This book is a revised and expanded version of notes based on lectures of the first author at UCLA over several \\Vinter Quarters, particularly 1986 and 1990. We owe Chris Bishop a great deal of gratitude for supervising the production of cour...

  14. Revised dietary guidelines for Koreans.

    Science.gov (United States)

    Jang, Young Ai; Lee, Haeng Shin; Kim, Bok Hee; Lee, Yoonna; Lee, Hae Jeung; Moon, Jae Jin; Kim, Cho-il

    2008-01-01

    With rapidly changing dietary environment, dietary guidelines for Koreans were revised and relevant action guides were developed. First, the Dietary Guidelines Advisory Committee was established with experts and government officials from the fields of nutrition, preventive medicine, health promotion, agriculture, education and environment. The Committee set dietary goals for Koreans aiming for a better nutrition state of all after a thorough review and analysis of recent information related to nutritional status and/or problems of Korean population, changes in food production/supply, disease pattern, health policy and agricultural policy. Then, the revised dietary guidelines were proposed to accomplish these goals in addition to 6 different sets of dietary action guides to accommodate specific nutrition and health problems of respective age groups. Subsequently, these guidelines and guides were subjected to the focus group review, consumer perception surveys, and a public hearing for general and professional comments. Lastly, the language was clarified in terms of public understanding and phraseology. The revised Dietary guidelines for Koreans are as follows: eat a variety of grains, vegetables, fruits, fish, meat, poultry and dairy products; choose salt-preserved foods less, and use less salt when you prepare foods; increase physical activity for a healthy weight, and balance what you eat with your activity; enjoy every meal, and do not skip breakfast; if you drink alcoholic beverages, do so in moderation; prepare foods properly, and order sensible amounts; enjoy our rice-based diet.

  15. Revision of hip resurfacing arthroplasty.

    Science.gov (United States)

    Wera, Glenn D; Gillespie, Robert J; Petty, Carter; Petersilge, William J; Kraay, Matthew J; Goldberg, Victor M

    2010-08-01

    Metal-on-metal (MOM) hip resurfacing has become an increasingly popular treatment for young, active patients with degenerative disease of the hip, as bearing surfaces with better wear properties are now available. One proposed advantage of resurfacing is its ability to be successfully revised to total hip arthroplasty (THA). In addition, radiographic parameters that may predict failure in hip resurfacing have yet to be clearly defined. Seven MOM resurfacing arthroplasties were converted to conventional THAs because of aseptic failure. Using Harris Hip Scores (HHS) and Short Form 12 (SF-12) questionnaire scores, we compared the clinical outcomes of these patients with those of patients who underwent uncomplicated MOM hip resurfacing. In addition, all revisions were radiographically evaluated. Mean follow-up periods were 51 months (revision group) and 43 months (control group). There was no significant difference between the 2 groups' HHS or SF-12 scores. There was no dislocation or aseptic loosening after conversion of any resurfacing arthroplasty. Valgus neck-shaft angle (P hip resurfacing. Conversion of aseptic failure of hip resurfacing to conventional THA leads to clinical outcomes similar to those of patients who undergo uncomplicated hip resurfacing. The orientation of the femur and the components placed play a large role in implant survival in hip resurfacing. More work needs to be done to further elucidate these radiographic parameters.

  16. Adult educators' core competences

    Science.gov (United States)

    Wahlgren, Bjarne

    2016-06-01

    Which competences do professional adult educators need? This research note discusses the topic from a comparative perspective, finding that adult educators' required competences are wide-ranging, heterogeneous and complex. They are subject to context in terms of national and cultural environment as well as the kind of adult education concerned (e.g. basic education, work-related education etc.). However, it seems that it is possible to identify certain competence requirements which transcend national, cultural and functional boundaries. This research note summarises these common or "core" requirements, organising them into four thematic subcategories: (1) communicating subject knowledge; (2) taking students' prior learning into account; (3) supporting a learning environment; and (4) the adult educator's reflection on his or her own performance. At the end of his analysis of different competence profiles, the author notes that adult educators' ability to train adult learners in a way which then enables them to apply and use what they have learned in practice (thus performing knowledge transfer) still seems to be overlooked.

  17. Challenges and Demands on Automated Software Revision

    Science.gov (United States)

    Bonakdarpour, Borzoo; Kulkarni, Sandeep S.

    2008-01-01

    In the past three decades, automated program verification has undoubtedly been one of the most successful contributions of formal methods to software development. However, when verification of a program against a logical specification discovers bugs in the program, manual manipulation of the program is needed in order to repair it. Thus, in the face of existence of numerous unverified and un- certified legacy software in virtually any organization, tools that enable engineers to automatically verify and subsequently fix existing programs are highly desirable. In addition, since requirements of software systems often evolve during the software life cycle, the issue of incomplete specification has become a customary fact in many design and development teams. Thus, automated techniques that revise existing programs according to new specifications are of great assistance to designers, developers, and maintenance engineers. As a result, incorporating program synthesis techniques where an algorithm generates a program, that is correct-by-construction, seems to be a necessity. The notion of manual program repair described above turns out to be even more complex when programs are integrated with large collections of sensors and actuators in hostile physical environments in the so-called cyber-physical systems. When such systems are safety/mission- critical (e.g., in avionics systems), it is essential that the system reacts to physical events such as faults, delays, signals, attacks, etc, so that the system specification is not violated. In fact, since it is impossible to anticipate all possible such physical events at design time, it is highly desirable to have automated techniques that revise programs with respect to newly identified physical events according to the system specification.

  18. Geochemical Constraints on Core Formation in the Earth

    Science.gov (United States)

    Jones, John H.; Drake, Michael J.

    1986-01-01

    New experimental data on the partitioning of siderophile and chalcophile elements among metallic and silicate phases may be used to constrain hypotheses of core formation in the Earth. Three current hypotheses can explain gross features of mantle geochemistry, but none predicts siderophile and chalcophile element abundances to within a factor of two of observed values. Either our understanding of metal-silicate interactions and/or our understanding of the early Earth requires revision.

  19. Mid-term results after implantation of rotating-hinge knee prostheses: primary versus revision

    Directory of Open Access Journals (Sweden)

    Turgay Efe

    2012-12-01

    Full Text Available The aim of the study was to evaluate the mid-term clinical results and survivorship of a rotating-hinge knee prosthesis (LINK® Endo-Model in difficult primary and complex revision situations. Results after primary implantation were compared with those of revision procedures. Forty-nine prostheses in 45 patients were reviewed clinically during follow up. Twenty-one of these were implanted in primary and 28 in revision situations. Outcome was evaluated using commonly used scores (Knee Society, UCLA Activity, Lequesne and a visual analog scale after a mean follow up of 56±37 months for 49 prostheses. Implant survival was analyzed using the Kaplan-Meier method. There were no significant differences in clinical examination and evaluation scores between the two groups (P>0.05. Survival rates at final follow up were 95% after primary implantation and 76% in revision procedures. The risk of prosthesis loss (odds ratio 5.7 was significantly higher after revision procedures (P=0.004. These data suggest that rotating-hinge knee prostheses provided good clinical and functional results in selected cases of advanced primary gonarthrosis associated with severe bone loss, ligamentous instability or comminuted fractures. They also provide good results in revision situations. However, the failure rate was significantly higher in cases of revision surgery.

  20. Structure and dynamics of core-periphery networks

    CERN Document Server

    Csermely, Peter; Wu, Ling-Yun; Uzzi, Brian

    2013-01-01

    Recent studies uncovered important core/periphery network structures characterizing complex sets of cooperative and competitive interactions between network nodes, be they proteins, cells, species or humans. Better characterization of the structure, dynamics and function of core/periphery networks is a key step of our understanding cellular functions, species adaptation, social and market changes. Here we summarize the current knowledge of the structure and dynamics of "traditional" core/periphery networks, rich-clubs, nested, bow-tie and onion networks. Comparing core/periphery structures with network modules, we discriminate between global and local cores. The core/periphery network organization lies in the middle of several extreme properties, such as random/condensed structures, clique/star configurations, network symmetry/asymmetry, network assortativity/disassortativity, as well as network hierarchy/anti-hierarchy. These properties of high complexity together with the large degeneracy of core pathways e...

  1. The core paradox.

    Science.gov (United States)

    Kennedy, G. C.; Higgins, G. H.

    1973-01-01

    Rebuttal of suggestions from various critics attempting to provide an escape from the seeming paradox originated by Higgins and Kennedy's (1971) proposed possibility that the liquid in the outer core was thermally stably stratified and that this stratification might prove a powerful inhibitor to circulation of the outer core fluid of the kind postulated for the generation of the earth's magnetic field. These suggestions are examined and shown to provide no reasonable escape from the core paradox.

  2. K-core inflation

    OpenAIRE

    Alexander L. Wolman

    2011-01-01

    K-core inflation is a new class of underlying inflation measures. The two most popular measures of underlying inflation are core inflation and trimmed mean inflation. The former removes fixed categories of goods and services (food and energy) from the inflation calculation, and the latter removes fixed percentiles of the weighted distribution of price changes. In contrast, k-core inflation specifies a size of relative price change to be removed from the inflation calculation. Thus, the catego...

  3. On the internal dynamics of starless cores: stability of starless cores with internal motions and collapse dynamics

    CERN Document Server

    Seo, Young Min; Shirley, Yancy L

    2013-01-01

    In order to understand the collapse dynamics of observed low-mass starless cores, we revise the conventional stability condition of hydrostatic Bonnor-Ebert spheres to take internal motions into account. Because observed starless cores resemble Bonnor-Ebert density structures, the stability and dynamics of the starless cores are frequently analyzed by comparing to the conventional stability condition of a hydrostatic Bonnor-Ebert sphere. However, starless cores are not hydrostatic but have observed internal motions. In this study, we take gaseous spheres with a homologous internal velocity field and derive stability conditions of the spheres utilizing a virial analysis. We propose two limiting models of spontaneous gravitational collapse: the collapse of critical Bonnor-Ebert spheres and uniform density spheres. The collapse of these two limiting models are intended to provide the lower and the upper limits, respectively, of the infall speeds for a given density structure. The results of our study suggest tha...

  4. Sediment Core Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Provides instrumentation and expertise for physical and geoacoustic characterization of marine sediments. DESCRIPTION: The multisensor core logger measures...

  5. Sediment Core Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Provides instrumentation and expertise for physical and geoacoustic characterization of marine sediments.DESCRIPTION: The multisensor core logger measures...

  6. Detecting Communities by Revised Max-flow Method in Networks

    Institute of Scientific and Technical Information of China (English)

    LIU Chuan-Jian; ZHU Zhi-Qiang; WU Jian-Liang

    2013-01-01

    A ubiquitous phenomenon in networks is the presence of communities within which the network connections are dense and between which they are sparser.This paper proposes a max-flow algorithm in bipartite networks to detect communities in general networks.Firstly,we construct a bipartite network in accordance with a general network and derive a revised max-flow problem in order to uncover the community structure.Then we present a local heuristic algorithm to find the optimal solution of the revised max-flow problem.This method is applied to a variety of real-world and artificial complex networks,and the partition results confirm its effectiveness and accuracy.

  7. Complexity, Reliability, and Design: Manufacturing Implications (Revised Version)

    OpenAIRE

    Ayres, R.U.

    1987-01-01

    A major component of IIASA's Technology-Economy-Society (TES) Program is a project to assess "Computer Integrated Manufacturing" (CIM), by which is meant the whole range of application of computers to discrete parts manufacturing and assembly. The various familiar acronyms and buzzwords, such as NC, CNC, DNC, CAD/CAM robotics, FMS, "group technology" and MRP all fit under the broad CIM umbrella. The present paper is the first to be generated, at least in part, under the project. (In fact, an ...

  8. A taxonomic revision of the Wallemia sebi species complex

    DEFF Research Database (Denmark)

    Jančič, Sašo; Nguyen, Hai D. T.; Frisvad, Jens Christian;

    2015-01-01

    Wallemia sebi is a xerophilic food- and air-borne fungus. The name has been used for strains that prevail in cold, temperate and tropical climates. In this study, multi-locus phylogenetic analyses, using the internal transcribed spacer (ITS) regions, DNA replication licensing factor (MCM7), pre-r...... of physiological, micromorphological and culture characters....

  9. Revision of FMM-Yukawa: An adaptive fast multipole method for screened Coulomb interactions

    Science.gov (United States)

    Zhang, Bo; Huang, Jingfang; Pitsianis, Nikos P.; Sun, Xiaobai

    2010-12-01

    revised and re-organized in data structure, software architecture, programming methods, and user interface. The revision enables more flexible use of the package and economic use of memory resources. It consists of five stages. The initial stage (stage 1) determines, based on the accuracy requirement and FMM theory, the length of multipole expansions and the number of quadrature points for diagonalization, and loads the quadrature nodes and weights that are computed off line. Stage 2 constructs the oct-tree and interaction lists, with adaptation to the sparsity or density of particles and employing a dynamic memory allocation scheme at every tree level. Stage 3 executes the core FMM subroutine for numerical calculation of the particle interactions. The subroutine can now be used iteratively as in a solver, while the particle locations remain the same. Stage 4 releases the memory allocated in Stage 2 for the adaptive tree and interaction lists. The user can modify the iterative routine easily. When the particle locations are changed such as in a molecular dynamics simulation, stage 2 to 4 can also be used together repeatedly. The final stage releases the memory space used for the quadrature and other remaining FMM parameters. Programs at the stage level and at the user interface are re-written in the C programming language, while most of the translation and interaction operations remain in FORTRAN. As a result of the change in data structures and memory allocation, the revised package can accommodate much larger particle ensembles while maintaining the same accuracy-efficiency performance. The new version is also developed as an important precursor to its parallel counterpart on multi-core or many core processors in a shared memory programming environment. Particularly, in order to ensure mutual exclusion in concurrent updates without incurring extra latency, we have replaced all the assignment statements at a source box that put its data to multiple target boxes with

  10. Structure of Hot Molecular Cores

    OpenAIRE

    Rolffs, Rainer

    2011-01-01

    High-mass stars form deeply embedded in dense molecular gas, which they heat up and ionize due to their high energy output. During an early phase, the ionization is confined to small regions, and the stellar radiation is absorbed by dust. The high temperatures lead to the evaporation of ice mantles around dust grains, and many highly excited and complex molecules can be observed in these Hot Molecular Cores. At later stages, the whole molecular cloud is ionized and disrupted, and a...

  11. My revision notes Edexcel GCSE computer science

    CERN Document Server

    Cushing, Steve

    2015-01-01

    Unlock your full potential with this revision guide which focuses on the key content and skills you need to know. With My Revision Notes for AQA GCSE Computer Science, which perfectly matches the latest examined elements of the course, you can: - Take control of your revision: plan and focus on the areas you need to revise, with advice, summaries and notes from author Steve Cushing - Show you fully understand key topics by using specific strategies and theories to add depth to your knowledge of programming and computing issues and processes - Apply programming and com

  12. My revision notes AQA GCSE English Language

    CERN Document Server

    Brindle, Keith

    2015-01-01

    Unlock your full potential with this revision guide for the new AQA GCSE English Language which focuses on the key content and skills you need to know. -Take control of your revision: plan and focus on the areas you need to revise. -Improve your exam skills with self-testing and exam-style questions. -Keep focused on what you need to know with Key Terms based around the exam questions. -Unpick each exam question with guidance on how to approach them and specific Revision Tasks

  13. Introduction to complex analysis

    CERN Document Server

    Priestley, H A

    2003-01-01

    Complex analysis is a classic and central area of mathematics, which is studied and exploited in a range of important fields, from number theory to engineering. Introduction to Complex Analysis was first published in 1985, and for this much awaited second edition the text has been considerably expanded, while retaining the style of the original. More detailed presentation is given of elementary topics, to reflect the knowledge base of current students. Exercise sets have beensubstantially revised and enlarged, with carefully graded exercises at the end of each chapter.This is the latest additi

  14. Evaluation of Fostering Students' Creativity in Preparing Aided Recalls for Revision Courses Using Electronic Revision and Recapitulation Tools 2.0

    Science.gov (United States)

    Christ, Oliver; Weber, Christoph; Sato, Toshihiko

    2012-01-01

    In this study, the electronic revision and recapitulation tools 2.0 (EREP 2.0) were used to foster creative moments while creating aided recalls (ARs) (pictures electronic notes etc.). Creative and critical thinking is associated with vital skills which enable students to deal with often complex knowledge domains through an informal way of…

  15. Revised

    DEFF Research Database (Denmark)

    Johannsen, Vivian Kvist; Nord-Larsen, Thomas; Riis-Nielsen, Torben

    and Energy and the Ministry of Environment. The report is based on the data from the Danish National Forest Inventory (NFI), performed for the Ministry of Environment and the SINKS project in relation to Article 3.4 of the Kyoto protocol for the Ministry of Climate and Energy. Forest & Landscape, Copenhagen...

  16. Ice Core Investigations

    Science.gov (United States)

    Krim, Jessica; Brody, Michael

    2008-01-01

    What can glaciers tell us about volcanoes and atmospheric conditions? How does this information relate to our understanding of climate change? Ice Core Investigations is an original and innovative activity that explores these types of questions. It brings together popular science issues such as research, climate change, ice core drilling, and air…

  17. Making an Ice Core.

    Science.gov (United States)

    Kopaska-Merkel, David C.

    1995-01-01

    Explains an activity in which students construct a simulated ice core. Materials required include only a freezer, food coloring, a bottle, and water. This hands-on exercise demonstrates how a glacier is formed, how ice cores are studied, and the nature of precision and accuracy in measurement. Suitable for grades three through eight. (Author/PVD)

  18. Ice Core Investigations

    Science.gov (United States)

    Krim, Jessica; Brody, Michael

    2008-01-01

    What can glaciers tell us about volcanoes and atmospheric conditions? How does this information relate to our understanding of climate change? Ice Core Investigations is an original and innovative activity that explores these types of questions. It brings together popular science issues such as research, climate change, ice core drilling, and air…

  19. Iowa Core Annual Report

    Science.gov (United States)

    Iowa Department of Education, 2015

    2015-01-01

    One central component of a great school system is a clear set of expectations, or standards, that educators help all students reach. In Iowa, that effort is known as the Iowa Core. The Iowa Core represents the statewide academic standards, which describe what students should know and be able to do in math, science, English language arts, and…

  20. Mercury's core evolution

    Science.gov (United States)

    Deproost, Marie-Hélène; Rivoldini, Attilio; Van Hoolst, Tim

    2016-10-01

    Remote sensing data of Mercury's surface by MESSENGER indicate that Mercury formed under reducing conditions. As a consequence, silicon is likely the main light element in the core together with a possible small fraction of sulfur. Compared to sulfur, which does almost not partition into solid iron at Mercury's core conditions and strongly decreases the melting temperature, silicon partitions almost equally well between solid and liquid iron and is not very effective at reducing the melting temperature of iron. Silicon as the major light element constituent instead of sulfur therefore implies a significantly higher core liquidus temperature and a decrease in the vigor of compositional convection generated by the release of light elements upon inner core formation.Due to the immiscibility in liquid Fe-Si-S at low pressure (below 15 GPa), the core might also not be homogeneous and consist of an inner S-poor Fe-Si core below a thinner Si-poor Fe-S layer. Here, we study the consequences of a silicon-rich core and the effect of the blanketing Fe-S layer on the thermal evolution of Mercury's core and on the generation of a magnetic field.

  1. Mars' core and magnetism.

    Science.gov (United States)

    Stevenson, D J

    2001-07-12

    The detection of strongly magnetized ancient crust on Mars is one of the most surprising outcomes of recent Mars exploration, and provides important insight about the history and nature of the martian core. The iron-rich core probably formed during the hot accretion of Mars approximately 4.5 billion years ago and subsequently cooled at a rate dictated by the overlying mantle. A core dynamo operated much like Earth's current dynamo, but was probably limited in duration to several hundred million years. The early demise of the dynamo could have arisen through a change in the cooling rate of the mantle, or even a switch in convective style that led to mantle heating. Presently, Mars probably has a liquid, conductive outer core and might have a solid inner core like Earth.

  2. Rich-cores in networks

    CERN Document Server

    Ma, Athen

    2014-01-01

    A core is said to be a group of central and densely connected nodes which governs the overall behavior of a network. Profiling this meso--scale structure currently relies on a limited number of methods which are often complex, and have scalability issues when dealing with very large networks. As a result, we are yet to fully understand its impact on network properties and dynamics. Here we introduce a simple method to profile this structure by combining the concepts of core/periphery and rich-club. The key challenge in addressing such association of the two concepts is to establish a way to define the membership of the core. The notion of a "rich-club" describes nodes which are essentially the hub of a network, as they play a dominating role in structural and functional properties. Interestingly, the definition of a rich-club naturally emphasizes high degree nodes and divides a network into two subgroups. Our approach theoretically couples the underlying principle of a rich-club with the escape time of a rand...

  3. The t-core of an s-core

    OpenAIRE

    Fayers, Matthew

    2010-01-01

    We consider the $t$-core of an $s$-core partition, when $s$ and $t$ are coprime positive integers. Olsson has shown that the $t$-core of an $s$-core is again an $s$-core, and we examine certain actions of the affine symmetric group on $s$-cores which preserve the $t$-core of an $s$-core. Along the way, we give a new proof of Olsson's result. We also give a new proof of a result of Vandehey, showing that there is a simultaneous $s$- and $t$-core which contains all others.

  4. The t-core of an s-core

    OpenAIRE

    Fayers, Matthew

    2010-01-01

    We consider the $t$-core of an $s$-core partition, when $s$ and $t$ are coprime positive integers. Olsson has shown that the $t$-core of an $s$-core is again an $s$-core, and we examine certain actions of the affine symmetric group on $s$-cores which preserve the $t$-core of an $s$-core. Along the way, we give a new proof of Olsson's result. We also give a new proof of a result of Vandehey, showing that there is a simultaneous $s$- and $t$-core which contains all others.

  5. Coralline hydroxyapatite in complex acetabular reconstruction.

    Science.gov (United States)

    Wasielewski, Ray C; Sheridan, Kate C; Lubbers, Melissa A

    2008-04-01

    This retrospective study examined whether a coralline hydroxyapatite bone graft substitute adequately repaired bone defects during complex acetabular reconstructions. Seventeen patients who underwent acetabular revision using Pro Osteon 500 were assessed to determine whether any cups required re-revision, whether bone had incorporated into the coralline hydroxyapatite grafts, and whether the coralline hydroxyapatite grafts resorbed with time. At latest follow-up, no cups required re-revision, but 1 had failed. Radiographic evidence of bone incorporation was observed in every coralline hydroxyapatite graft. Graft resorption was not observed.

  6. Recovery of antigenically reactive HIV-2 cores.

    Science.gov (United States)

    Chrystie, I L; Almeida, J D

    1989-03-01

    Negative staining studies of human immunodeficiency virus (HIV) have been hampered by the fragile nature of the particles. Although detergent treatment is capable of releasing cores from HIV-2 particles, these are unstable and do not retain morphological integrity. Addition of glutaraldehyde will stabilise these structures but, if used at too high a concentration, will destroy their antigenicity. This study shows that if both detergent and glutaraldehyde are used in correct proportions, antigenically reactive cores can be recovered from HIV-2 cell cultures. More specifically we show that a mixture of 0.1% Nonidet P40 and 0.1% glutaraldehyde produces preparations of HIV-2 cores that are suitable for immune electron microscopy. These cores reacted positively, that is, formed immune complexes, with both human HIV-2 antisera and a mouse monoclonal antibody that, although directed against p24 (HIV-1), reacts also with p25 (HIV-2).

  7. Korrelasjon mellom core styrke, core stabilitet og utholdende styrke i core

    OpenAIRE

    Berg-Olsen, Andrea Marie; Fugelsøy, Eivor; Maurstad, Ann-Louise

    2010-01-01

    Formålet med studien var å se hvilke korrelasjon det er mellom core styrke, core stabilitet og utholdende styrke i core. Testingen bestod av tre hoveddeler hvor vi testet core styrke, core stabilitet og utholdende styrke i core. Innenfor core styrke og utholdende styrke i core ble tre ulike tester utført. Ved måling av core stabilitet ble det gjennomført kun en test. I core styrke ble isometrisk abdominal fleksjon, isometrisk rygg ekstensjon og isometrisk lateral fleksjon testet. Sit-ups p...

  8. Korrelasjon mellom core styrke, core stabilitet og utholdende styrke i core

    OpenAIRE

    Berg-Olsen, Andrea Marie; Fugelsøy, Eivor; Maurstad, Ann-Louise

    2010-01-01

    Formålet med studien var å se hvilke korrelasjon det er mellom core styrke, core stabilitet og utholdende styrke i core. Testingen bestod av tre hoveddeler hvor vi testet core styrke, core stabilitet og utholdende styrke i core. Innenfor core styrke og utholdende styrke i core ble tre ulike tester utført. Ved måling av core stabilitet ble det gjennomført kun en test. I core styrke ble isometrisk abdominal fleksjon, isometrisk rygg ekstensjon og isometrisk lateral fleksjon testet. Sit-ups p...

  9. Earth's inner core: Innermost inner core or hemispherical variations?

    NARCIS (Netherlands)

    Lythgoe, K. H.; Deuss, A.; Rudge, J. F.; Neufeld, J. A.

    2014-01-01

    The structure of Earth's deep inner core has important implications for core evolution, since it is thought to be related to the early stages of core formation. Previous studies have suggested that there exists an innermost inner core with distinct anisotropy relative to the rest of the inner core.

  10. Earth's inner core: Innermost inner core or hemispherical variations?

    NARCIS (Netherlands)

    Lythgoe, K. H.; Deuss, A.; Rudge, J. F.; Neufeld, J. A.

    2014-01-01

    The structure of Earth's deep inner core has important implications for core evolution, since it is thought to be related to the early stages of core formation. Previous studies have suggested that there exists an innermost inner core with distinct anisotropy relative to the rest of the inner core.

  11. Revision of the genus Endospermum Bth. (Euphorbiaceae)

    NARCIS (Netherlands)

    Schaeffer, J.

    1971-01-01

    The revision was undertaken because the latest monograph by Pax & Hoffmann, dating from 1912, did not provide a satisfactory key, and because since that time a very large amount of new material has been collected and several new species were described. In the present revision 12 species have been re

  12. Femoral revision surgery with impaction bone grafting

    NARCIS (Netherlands)

    B.L.E.F. ten Have (Bas); R.W. Brouwer (Reinoud); F.C. van Biezen (Frans); J.A.N. Verhaar (Jan)

    2012-01-01

    textabstractThe purpose of this prospective study was to evaluate the long-term clinical and radiological outcomes of revision of the femoral component of a total hip replacement using impaction bone grafting. Femoral revision with an impacted allograft was performed on 29 patients (31 hips). In all

  13. A taxonomic revision of Harpullia (Sapindaceae)

    NARCIS (Netherlands)

    Leenhouts, P.W.; Vente, Magda

    1982-01-01

    The present taxonomic revision of Harpullia was started by the second author as the main part of her work for a M. Sc. in biology at Leiden University. She concentrated on a revision of the species occurring in New Guinea, paid only a more superficial attention to the rest of the genus. The first

  14. Evaluating Writing: Effects of Feedback on Revision.

    Science.gov (United States)

    Chaudron, Craig

    The effect of evaluation method on English as second language (ESL) learners' revisions of their compositions was investigated. Teacher comments, peer evaluations, and English-speaking peer reformulations were compared. Judges rated the revised compositions of 9 advanced and 14 intermediate college ESL students using the ESL Composition Profile.…

  15. Revised State Budget Sells Kids Short

    Science.gov (United States)

    Children Now, 2012

    2012-01-01

    The Administration's May Revision of the 2012-2013 state budget addresses a $15.7 billion shortfall through funding shifts, cuts, and new revenue sources that place children squarely in harms way. California's kids are already grossly underserved relative to the rest of the nation's children. If the May Revise budget is passed by the Legislature,…

  16. Postoperative pain treatment' practice guideline revised

    NARCIS (Netherlands)

    Houweling, P.L.; Molag, M.L.; Boekel, R.L.M. van; Verbrugge, S.J.; Haelst, I.M. van; Hollmann, M.W.

    2013-01-01

    - On the initiative of the Dutch Association of Anaesthesiologists, a multidisciplinary workgroup has revised the 2003 practice guideline on 'Postoperative pain treatment' for adults and children.- The main reason for revision was the availability of new drugs and new methods of administration. The

  17. English Learners, Writing, and the Common Core

    Science.gov (United States)

    Olson, Carol Booth; Scarcella, Robin; Matuchniak, Tina

    2015-01-01

    Adopted by 46 states, the Common Core State Standards (CCSS) present a vision of what it means to be literate in the twenty-first century and call for all students, including English learners, to develop critical reading skills necessary for a deep understanding of complex texts, and critical writing skills to write about those texts. This article…

  18. English Learners, Writing, and the Common Core

    Science.gov (United States)

    Olson, Carol Booth; Scarcella, Robin; Matuchniak, Tina

    2015-01-01

    Adopted by 46 states, the Common Core State Standards (CCSS) present a vision of what it means to be literate in the twenty-first century and call for all students, including English learners, to develop critical reading skills necessary for a deep understanding of complex texts, and critical writing skills to write about those texts. This article…

  19. Common Core State Standards and Adaptive Teaching

    Science.gov (United States)

    Kamil, Michael L.

    2016-01-01

    This article examines the issues of how Common Core State Standards (CCSS) will impact adaptive teaching. It focuses on 2 of the major differences between conventional standards and CCSS: the increased complexity of text and the addition of disciplinary literacy standards to reading instruction. The article argues that adaptive teaching under CCSS…

  20. Revised positions for the CIG galaxies

    CERN Document Server

    Leon, S

    2003-01-01

    We present revised positions for the 1051 galaxies belonging to the Karachentseva Catalog of Isolated Galaxies (CIG). New positions were calculated by applying SExtractor to the Digitized Sky Survey CIG fields with a spatial resolution of 1.2''. We visually checked the results and for 118 galaxies had to recompute the assigned positions due to complex morphologies (e.g. distorted isophotes, undefined nuclei, knotty galaxies) or the presence of bright stars. We found differences between older and newer positions of up to 38'' with a mean value of 2.96'' relative to SIMBAD and up to 38'' and 2.42'' respectively relative to UZC. Based on star positions from the APM catalog we determined that the DSS astrometry of five CIG fields has a mean offset in (RA, Dec) of (-0.90'',0.93'') with a dispersion of 0.4''. These results have been confirmed using the 2MASS All-Sky Catalog of Point Sources. The intrinsic errors of our method combined with the astrometric ones are of the order of 0.5''.