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Sample records for conventional tomato cropping

  1. Nine-year results on maize and processing tomato cultivation in an organic and in a conventional low input cropping system

    Directory of Open Access Journals (Sweden)

    Michela Farneselli

    2013-02-01

    Full Text Available Nine-year results on yields and apparent balances of organic matter and nitrogen (N are reported for maize and processing tomato cultivated in a long term comparison trial between an organic and a conventional low-input system in Central Italy. In every year, above ground biomass and N accumulation of each cash crop and green manure, including weeds, and the partitioning between marketable yield and crop residues were determined. Apparent dry matter and nitrogen balances were calculated at the end of each crop cycle by taking into account the amounts of dry matter and ex-novo N supplied to the system as green manure legume Ndfa (i.e. an estimate of N derived from the atmosphere via symbiotic fixation and fertilizers, and those removed with marketable yield. Processing tomato complied with organic cultivation better than maize. As compared to the conventional crop cultivation, organic tomato provided similar yields, used supplied N more efficiently and left lower residual N after harvest, with lower related risks of pollution. Organic maize yielded less than conventional one. The main limitation for organic maize was the low N availability during initial growth phases, due to either low N supply or low rate of N release from incorporated green manure biomass. In both organic and conventional cultivation the system sustainability could be improved by an appropriate crop rotation: wheat in fall winter likely prevented leaching loss of mineral N in both systems; green manure crops in the organic system allowed to either trap and recycle soil mineral N or supply ex novo legume Ndfa to the soil, with benefits in mitigation of N pollution and improvement in self-sufficiency of the system.

  2. Organic and conventional tomato cropping systems Sistemas de cultivo orgânico e convencional de tomateiro

    Directory of Open Access Journals (Sweden)

    Wagner Bettiol

    2004-06-01

    Full Text Available Among several alternative agricultural systems have been developed, organic agriculture has deserved increasing interest from. The objective of this paper was comparing both organic (OS and conventional (CS tomato cropping systems for varieties Débora and Santa Clara, through an interdisciplinary study. The experiment was set up in a randomized blocks design with six replicates, in a dystrophic Ultisol plots measuring 25 ´ 17 m. Cropping procedures followed by either local conventional or organic growers practices recommendations. Fertilization in the OS was done with organic compost, single superphosphate, dolomitic limes (5L, 60 g, and 60 g per pit, and sprayed twice a week with biofertilizer. Fertilization in the CS was done with 200 g 4-14-8 (NPK per pit and, after planting, 30 g N, 33 g K and 10.5 g P per pit; from 52 days after planting forth, plants were sprayed once a week with foliar fertilizer. In the CS, a blend of insecticides, fungicides and miticides was sprayed twice a week, after planting. In the OS, extracts of black pepper, garlic, and Eucalyptus; Bordeaux mixture, and biofertilizer, were applied twice a week to control diseases and pests. Tomato spotted wilt was the most important disease in the OS, resulting in smaller plant development, number of flower clusters and yield. In the CS, the disease was kept under control, and the population of thrips, the virus vector, occurred at lower levels than in the OS. Variety Santa Clara presented greater incidence of the viral disease, and for this reason had a poorer performance than 'Débora', especially in the OS. Occurrence of Liriomyza spp. was significantly smaller in the OS, possibly because of the greater frequency of Chrysoperla. The CS had smaller incidence of leaf spots caused by Septoria lycopersici and Xanthomonas vesicatoria. However, early blight and fruit rot caused by Alternaria solani occurred in larger numbers. No differences were observed with regard to the

  3. 7 CFR 457.160 - Processing tomato crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Processing tomato crop insurance provisions. 457.160... tomato crop insurance provisions. The Processing Tomato Crop Insurance Provisions for the 2005 and... polices: Processing Tomato Crop Provisions If a conflict exists among the policy provisions, the order...

  4. Evaluating the Ability of some Medicinal Plants for Controlling Rhizopus (Rhizopu snigricans and Black Spot Rot (Alternaria alternate as Postharvest Diseases in Tomato Produced under Conventional and Organic Cropping Systems

    Directory of Open Access Journals (Sweden)

    S.M Seyyedi

    2016-11-01

    Full Text Available Introduction After crops harvesting, conditions and durations of storage are considered as the most crucial factors formaintaining the nutritional value and quality of agro-horticultural products such as tomato (Lycopersicom esculentum Mill. and its waste reduction. However, the rhizopus rot (Rhizopus stolonifer and black spot rot (Alternaria alternate are the most important postharvest diseases in tomato during storage. In other word, among the factors reducing quality of the postharvest tomato, Rhizopus nigricans Ehrenb. (Rhizopus stolonifer and Alternaria alternate (Fr.:Fr. Keissl. f. sp. lycopersici paly a special role in the contaminated tomato fruits that can affect its taste, firmness and stiffness. In recent years, due to the problems and threats arising from the use of chemical fungicides in agricultural systems, principled management of alternative biological approaches for reducing the postharvest contamination in tomato, especially during storage, is emphasized more than ever. Considering these conditions, the current study was aimed to investigate the effects of some medicinal plants including thyme (Thymus vulgaris L., pennyroyal (Mentha pulegium L., peppermint (Mentha piperita L., eucalyptus (Eucalyptus globules L., caster bean (Ricinus communis L. and tomato in their ability to control the rhizopus (Rhizopus nigricans and black spot rot (Alternaria alternate in tomato production under conventional and organic cropping systems. Materials and methods The experiment was conducted at Faculty of Agriculture, Ferdowsi University of Mashhad, Iran, during theyear of 2010. A completely randomized design was used based on factorial arrangement with three replications and 14 treatments. Two cropping production systems (conventional and organic and seven medicinal plants (thyme, pennyroyal, peppermint, eucalyptus, caster bean, tomato and control were the first and the second experimental factors, respectively. After collecting plant samples

  5. Qualidade do tomate de mesa cultivado nos sistemas convencional e orgânico Quality of tomatoes cultivated in the organic and conventional cropping systems

    Directory of Open Access Journals (Sweden)

    Sila Mary Rodrigues Ferreira

    2010-03-01

    Full Text Available A presença dos produtos orgânicos nas gôndolas das grandes redes de supermercados indica que existe um potencial de mercado para esses produtos, no entanto poucas são as informações técnico-científicas sobre eles. Em razão disso, o presente trabalho teve como objetivo determinar a qualidade do tomate de mesa cultivado nos sistemas convencional (SC e orgânico (SO comercializado na Região Metropolitana de Curitiba. As amostras foram avaliadas pela massa, peso específico, cinzas, sólidos totais, sólidos solúveis totais, acidez titulável total, relação sólidos solúveis totais/acidez titulável total, pH, vitamina C, nitratos, nitritos, multirresíduos, benzimidazóis e ditiocarbamatos. Os resultados da análise físico-química mostraram que somente na umidade os tomates não apresentaram diferença significativa ao nível de 5% quando comparados pelo Teste de Tukey. Em relação à análise toxicológica, não foram detectados multirresíduos e benzimidazóis até os limites de 0,04 mg.kg-1 e 0,1 mg.kg-1 de carbendazim, respectivamente. Nos resíduos de pesticidas do grupo químico ditiocarbamatos, foi identificado 0,01 mg.kg-1 (CS2 nas amostras de SC3 e SC4 do tomate de mesa cultivado no sistema convencional, abaixo do limite máximo recomendado (LMR de 2,0 mg.kg-1 (CS2 de mancozebe.The presence of organic products in the aisles of big supermarket chains indicates a market potential for these products. However, there is little technical and scientific information. Thus, the objective of this work was to determine the quality of tomatoes grown under conventional (CS and organic (OS systems, on sale in the metropolitan area of Curitiba, Brazil. Samples were evaluated in terms of mass, specific weight, ash, total solids, total soluble solids, total titratable acidity, total soluble solids/total titratable acidity ratio, pH, vitamin C, nitrates, nitrites, multiresidues. There were no significant differences between conventional and

  6. Nutritional properties of cherry tomatoes harvested at different times and grown in an organic cropping.

    OpenAIRE

    Pinho, L.; Almeida, A. C.; Costa, C.A.; PAES, M. C. D.; M.B.A. Glória; Souza, R. M.

    2011-01-01

    The physicochemical characteristics of the cherry tomato cultivated in organic and conventional production systems and harvested at either 30 or 45 days of cropping were evaluated using a randomized, 2x2 factorial design (2 cropping systems x 2 harvesting times) with five repetitions. The parameters analyzed were color, centesimal composition, total energetic value, carotenoids and bioactive amine content. Tomatoes harvested at 30 days had higher total soluble solid (TSS) content when grown c...

  7. Classificação do tomate de mesa cultivado nos sistemas convencional e orgânico Classification of the tomato cultivated in the organic and conventional cropping systems

    Directory of Open Access Journals (Sweden)

    Sila Mary Rodrigues Ferreira

    2005-09-01

    Full Text Available O trabalho teve como objetivo realizar a classificação dos tomates de mesa (Licopersicon esculentum Mill. convencional e orgânico comercializados na Região Metropolitana de Curitiba. Foram avaliadas seis amostras de tomates do sistema convencional e três de tomates do sistema orgânico. Os frutos foram classificados em: grupos, oblongo e redondo; subgrupos: verde maduro, pintado, rosado, vermelho e vermelho maduro e classes: gigante, grande, médio e pequeno. De acordo com a ocorrência de defeitos graves e leves nos frutos, os tomates foram classificados também em tipos: extra, especial ou selecionado e comercial. As amostras cultivadas no sistema convencional foram classificadas como do grupo redondo e plurilocular, enquanto que as do sistema orgânico do grupo oblongo e bilocular. Na classe houve um predomínio de frutos médios e nos subgrupos de frutos rosado e vermelho. Em relação ao tipo, todas as amostras foram classificadas como fora do padrão ou do tipo.The objective of this work was the classification of the tomato (Lycopersicon esculentum Mill. cultivated in the organic and conventional cropping systems and commercialized in the Metropolitan Area of Curitiba. Six samples of conventionally grown tomatoes and three samples of organic tomatoes were evaluated. The fruits were classified in groups (oblong and round, subgroups (mature green, breaker, pink, red and mature red, and classes (giant, big, medium and small. According to the occurrence of serious damage and small defects on the fruits, they were classified in types, which can be extra, special or selected and commercial. The samples of conventional systems were classified as round and with than two locules, while of organic systems was classified as oblong and with two locules. Concerning the classes, the medium fruits predominated in the pink and red subgroups. All the samples were classified as out of standard or type due to the occurrence of defects.

  8. Do Organic Cherry Vine Tomatoes Taste Better Than Conventional Cherry Vine Tomatoes? A Sensory and Instrumental Comparative Study from Ireland

    OpenAIRE

    Gilsenan, Clare; Burke, Roisin; Barry-Ryan, Catherine

    2011-01-01

    A consumer panel was able to distinguish a perceptible difference between organically farmed and conventionally produced tomatoes, and preferred the taste of the conventional tomatoes. The sensory evaluation results of the trained panel revealed that the conventional tomatoes were sweeter and less sour than the organic tomatoes. In addition to this, the conventional tomatoes showed significant differences for oBrix, reducing sugars and electrical conductivity. No significant differences were ...

  9. Tomato crop coefficient grown under mediterranean climate conditions

    DEFF Research Database (Denmark)

    Cerekovic, Natasa; Todorovic, Mladen; Snyder, Richard L.

    2011-01-01

    The measurements of the main weather parameters for tomato crop data were collected in 2002. at experimental meteorological station "E. Pantenelli" of Bari University and CNR-Bari near Policoro (Southern Italy) to investigate the influence of weather and management on crop growth and development...

  10. Herbicide and cover crop residue integration in conservation tillage tomato

    Science.gov (United States)

    The increased adoption of conservation tillage in vegetable production requires more information on the role of various cover crops in weed control, tomato quality, and yield. Three conservation-tillage systems utilizing crimson clover, turnip, and cereal rye as winter cover crops were compared to a...

  11. Evaluating two irrigation controllers under subsurface drip irrigated tomato crop

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    Hussein M. Al-Ghobari

    2016-12-01

    Full Text Available Smart systems could be used to improve irrigation scheduling and save water under Saudi Arabia’s present water crisis scenario. This study investigated two types of evapotranspiration-based smart irrigation controllers, SmartLine and Hunter Pro-C2, as promising tools for scheduling irrigation and quantifying plants’ water requirements to achieve water savings. The effectiveness of these technologies in reducing the amount of irrigation water was compared with the conventional irrigation scheduling method as a control treatment. The two smart irrigation sensors were used for subsurface irrigation of a tomato crop (cv. Nema in an arid region. The results showed that the smart controllers significantly reduced the amount of applied water and increased the crop yield. In general, the Hunter Pro-C2 system saved the highest amount of water and produced the highest crop yield, resulting in the highest water irrigation efficiency compared with the SmartLine controller and the traditional irrigation schedule. It can be concluded that the application of advanced scheduling irrigation techniques such as the Hunter controller under arid conditions can realise economic benefits by saving large amounts of irrigation water.

  12. Dinâmica populacional de Bemisia tabaci biótipo B em tomate monocultivo e consorciado com coentro sob cultivo orgânico e convencional Population dynamic of Benisua tabaci B biotype in monoculture tomato crop and consortium with coriander in organic and conventional crop system

    Directory of Open Access Journals (Sweden)

    Pedro HB Togni

    2009-06-01

    Full Text Available A mosca-branca Bemisia tabaci Biótipo B (Hemiptera: Aleyrodidae, é um herbívoro de difícil controle devido à alta plasticidade genotípica da espécie. No tomateiro pode causar danos severos principalmente pela transmissão de diversas viroses. O manejo do sistema de produção e o consórcio de culturas podem ter um efeito direto nas populações desse herbívoro, sem que seja necessária a aplicação de inseticidas. Avaliou-se a influência dos sistemas de produção orgânico e convencional e o consórcio tomate-coentro na dinâmica populacional da mosca-branca no campo experimental da Embrapa Hortaliças, de maio a setembro/06. O monitoramento dos adultos da mosca-branca e de seus inimigos naturais foi realizado utilizando-se armadilhas adesivas amarelas fixadas nas bordas e no interior das parcelas experimentais e a amostragem de ninfas foi realizada por observação direta das folhas de tomate no campo. Embora as populações ao redor dos diferentes tratamentos fossem equivalentes, a abundância de adultos de mosca-branca foi significativamente menor nas parcelas de tomate consorciado com coentro, tanto no sistema convencional como orgânico. Apenas o consórcio tomatecoentro em sistema orgânico apresentou redução significativa na quantidade de ninfas por planta em relação aos demais tratamentos. Os inimigos naturais foram significativamente mais abundantes em sistema orgânico e foi verificada uma correlação negativa da abundância dos inimigos naturais e a quantidade de ninfas por planta. A associação tomate-coentro e o manejo orgânico do agroecossistema favoreceram ao controle biológico natural da mosca-branca.Due to its high genotypic plasticity, the control of the silverleaf whitefly Bemisia tabaci B biotype (Hemiptera: Aleyrodidae, is difficult. This insect may cause severe damage to the tomato crop as a vector of several viruses. The management of the production system and the consortium with other crops may have a

  13. INTEGRATED WEED MANAGEMENT ON THE PROCESSING TOMATO CROP AND TOMATO FOR CONSUMPTION IN NATURA

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    Y. O. Castro

    2016-11-01

    Full Text Available Weeds cause direct and indirect damage to processing tomato and tomato for consumption in natura. The coexistence period is decisive for the intensity of damage, although the economic cost is also considered for decision making when to control the weeds. There are similarities between processing tomato and tomato for consumption in natura cropping system and peculiarities. This causes the management has adopted its common applications and its variables within each system. As control alternative, the farmer has basically the preventive control, mechanical, cultural, biological and chemical. The application of a single method is not recommended. Ideally, the methods needs to be integrated in order to combat weeds, highly evolved populations and resistant to unfavorable conditions. Consider weed management taking only one control measure is to underestimate the evolutionary ability of such species. Therefore, it is necessary to integrate the various methods available to the weed interference not impede the tomato production.

  14. Tomatoes

    NARCIS (Netherlands)

    Heuvelink, E.

    2005-01-01

    Tomatoes are one of the most widely produced and consumed horticultural crops in the world, both for the fresh produce market and the processed food industries. This book describes the scientific principles underlying the biology and production of the tomato crop, both in the open field and in green

  15. Emerging Viral Diseases of Tomato Crops

    NARCIS (Netherlands)

    Hanssen, I.M.; Lapidot, M.; Thomma, B.P.H.J.

    2010-01-01

    Viral diseases are an important limiting factor in many crop production systems. Because antiviral products are not available, control strategies rely on genetic resistance or hygienic measures to prevent viral diseases, or on eradication of diseased crops to control such diseases. Increasing intern

  16. Pick and Eat Crop Testing: Dwarf Tomato and Pepper as Candidate Space Crops

    Science.gov (United States)

    Wheeler, R. M.; Massa, G. D.; Stutte, G. W.; Spencer, L. E.; Hummerick, M. E.; Sirmons, T.; Douglas, G. L.

    2016-01-01

    Dwarf tomato and pepper plants were grown in controlled environment chambers to assess their potential as space crops for supplementing the crew's diet. Six cultivars of each species were compared in initial tests and then down-selected to three cultivars of each. Initial selection criteria included fruit yield, growth height, and nutritional value. Following completion of a second production test with the three best performing cultivars, sample fruits of both tomato and peppers were then assessed for acceptance using tasting panels. Based on the criteria considered in these studies, Red Robin tomato and Pompeii pepper were recommended for consideration for use in space.

  17. Pepino mosaic virus and Tomato chlorosis virus causing mixed infection in protected tomato crops in Sicily

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    SALVATORE DAVINO

    2008-07-01

    Full Text Available An unusual virus-like yellow leaf disorder associated with fruit marbling was observed during the winter of 2005 in some greenhouse tomato crops in the province of Ragusa Sicily (Southern Italy. Leaf samples from 250 symptomatic tomato plants were serologically tested by DAS-ELISA technique for 5 viruses: Tomato spotted wilt virus (TSWV, Impatiens necrotic spot virus (INSV, Tobacco mosaic virus (TMV, Cucumber mosaic virus (CMV and Pepino mosaic virus (PepMV. PepMV was detected in 215 of the samples. The virus was mechanically transmitted to cucumber, wild metel, wild tobacco and ‘Rio Grande’ tomato. The experimental host range of PepMV-Ragusa differed from that of the PepMV found in Sardinia in 2001, which infected ‘Camone’ tomato. By applying RT-PCR to 25 PepMV-infected tomato plants, the expected 844 bp DNA fragment for PepMV and the expected 439 bp DNA fragment for Tomato chlororis virus (ToCV were obtained from all the samples tested. Sequences of the obtained amplicons were used to study the phylogenetic relationships of the viruses with isolates from other countries. Nucleotide sequence alignments showed that the sequence CP-PepMV-Ragusa (Genbank acc. No. DQ 517884 were 99% homologous with both US2 and Spain-Murcia isolates, while those of ToCV-Ragusa (Genbank acc. No. DQ517885 isolate HSP70, were 99% homologous with the Florida isolate, and 98% with the Lebanon isolate. The results proved that the unusual disorder found in greenhouse tomatoes in Sicily can be associated with infections by PepMV and ToCV, reported for the first time in a mixed infection.

  18. 76 FR 71271 - Common Crop Insurance Regulations; Fresh Market Tomato (Dollar Plan) Crop Provisions

    Science.gov (United States)

    2011-11-17

    .... * * * * * Direct marketing. The sale of the insured crop directly to consumers without the intervention of an... Internet and other information technologies to provide increased opportunities for citizen access to... meet the Agricultural Marketing Service United States Standards for Grades of Fresh Tomatoes; and the...

  19. Physiological response and sulfur metabolism of the V. dahliae-infected tomato plants in tomato/potato onion companion cropping

    Science.gov (United States)

    Fu, Xuepeng; Li, Chunxia; Zhou, Xingang; Liu, Shouwei; Wu, Fengzhi

    2016-01-01

    Companion cropping with potato onions (Allium cepa var. agrogatum Don.) can enhance the disease resistance of tomato plants (Solanum lycopersicum) to Verticillium dahliae infection by increasing the expressions of genes related to disease resistance. However, it is not clear how tomato plants physiologically respond to V. dahliae infection and what roles sulfur plays in the disease-resistance. Pot experiments were performed to examine changes in the physiology and sulfur metabolism of tomato roots infected by V. dahliae under the companion cropping (tomato/potato onion). The results showed that the companion cropping increased the content of total phenol, lignin and glutathione and increased the activities of peroxidase, polyphenol oxidase and phenylalanine ammonia lyase in the roots of tomato plants. RNA-seq analysis showed that the expressions of genes involved in sulfur uptake and assimilation, and the formation of sulfur-containing defense compounds (SDCs) were up-regulated in the V. dahlia-infected tomatoes in the companion cropping. In addition, the interactions among tomato, potato onion and V. dahliae induced the expression of the high- affinity sulfate transporter gene in the tomato roots. These results suggest that sulfur may play important roles in tomato disease resistance against V. dahliae. PMID:27808257

  20. Tomato and cowpea crop evapotranspiration in an unheated greenhouse

    Institute of Scientific and Technical Information of China (English)

    Xu Junzeng; Peng Shizhang; Luo Yufeng; Jiao Xiyun

    2008-01-01

    With the development of protected cultivation of vegetables in China, it is necessary to study the water requirements of crops in greenhouses. Lysimeter experiments were carried out to investigate tomato (2001) and cowpea (2004) crop evapotranspiration (ETc) in an unheated greenhouse in Eastern China. Results showed remarkably reduced crop evapotranspiration inside the greenhouse as compared with that outside. ETc increased with the growth of the crops, and varied in accordance with the temperature inside the greenhouse and 20-cm pan evaporation outside, reaching its maximum value at the stage when plants' growth was most active. Differences between the variation of crop evapotranspiration and pan evaporation inside the greenhouse were caused by shading of the pan in the later period when the crops were taller than the location where the pan was installed, 70 cm above ground. The ratio of crop evapotranspiration to pan evaporation was not constant as reported in previous studies, and the variation of the inside ratio αin lagged behind that of the outside ratio αout. Simulation of crop evapotranspiration based on 20-cm pan evaporation inside the greenhouse is more reasonable than that based on 20-cm pan evaporation outside, although pan evaporation outside is more consistent with ETc than that inside. The value of αin, calculated based on air temperature, relative humidity, and ground temperature inside, plays a dominant role in the calculation of ETc. As the crop height increases, altering the location of the inside pan and placing it above the canopy, out of the shade, would help to achieve more reasonable values of crop evapotranspiration.

  1. Tomato and cowpea crop evapotranspiration in an unheated greenhouse

    Directory of Open Access Journals (Sweden)

    Xu Junzeng

    2008-06-01

    Full Text Available With the development of protected cultivation of vegetables in China, it is necessary to study the water requirements of crops in greenhouses. Lysimeter experiments were carried out to investigate tomato (2001 and cowpea (2004 crop evapotranspiration (ETc in an unheated greenhouse in Eastern China. Results showed remarkably reduced crop evapotranspiration inside the greenhouse as compared with that outside. ETc increased with the growth of the crops, and varied in accordance with the temperature inside the greenhouse and 20-cm pan evaporation outside, reaching its maximum value at the stage when plants’ growth was most active. Differences between the variation of crop evapotranspiration and pan evaporation inside the greenhouse were caused by shading of the pan in the later period when the crops were taller than the location where the pan was installed, 70 cm above ground. The ratio of crop evapotranspiration to pan evaporation was not constant as reported in previous studies, and the variation of the inside ratio αin lagged behind that of the outside ratio αout. Simulation of crop evapotranspiration based on 20-cm pan evaporation inside the greenhouse is more reasonable than that based on 20-cm pan evaporation outside, although pan evaporation outside is more consistent with ETc than that inside. The value of αin, calculated based on air temperature, relative humidity, and ground temperature inside, plays a dominant role in the calculation of ETc. As the crop height increases, altering the location of the inside pan and placing it above the canopy, out of the shade, would help to achieve more reasonable values of crop evapotranspiration.

  2. Role of soil, crop debris, and a plant pathogen in Salmonella enterica contamination of tomato plants.

    Directory of Open Access Journals (Sweden)

    Jeri D Barak

    Full Text Available BACKGROUND: In the U.S., tomatoes have become the most implicated vehicle for produce-associated Salmonellosis with 12 outbreaks since 1998. Although unconfirmed, trace backs suggest pre-harvest contamination with Salmonella enterica. Routes of tomato crop contamination by S. enterica in the absence of direct artificial inoculation have not been investigated. METHODOLOGY/PRINCIPAL FINDINGS: This work examined the role of contaminated soil, the potential for crop debris to act as inoculum from one crop to the next, and any interaction between the seedbourne plant pathogen Xanthomonas campestris pv. vesicatoria and S. enterica on tomato plants. Our results show S. enterica can survive for up to six weeks in fallow soil with the ability to contaminate tomato plants. We found S. enterica can contaminate a subsequent crop via crop debris; however a fallow period between crop incorporation and subsequent seeding can affect contamination patterns. Throughout these studies, populations of S. enterica declined over time and there was no bacterial growth in either the phyllosphere or rhizoplane. The presence of X. campestris pv. vesicatoria on co-colonized tomato plants had no effect on the incidence of S. enterica tomato phyllosphere contamination. However, growth of S. enterica in the tomato phyllosphere occurred on co-colonized plants in the absence of plant disease. CONCLUSIONS/SIGNIFICANCE: S. enterica contaminated soil can lead to contamination of the tomato phyllosphere. A six week lag period between soil contamination and tomato seeding did not deter subsequent crop contamination. In the absence of plant disease, presence of the bacterial plant pathogen, X. campestris pv. vesicatoria was beneficial to S. enterica allowing multiplication of the human pathogen population. Any event leading to soil contamination with S. enterica could pose a public health risk with subsequent tomato production, especially in areas prone to bacterial spot disease.

  3. Using genetically modified tomato crop plants with purple leaves for absolute weed/crop classification.

    Science.gov (United States)

    Lati, Ran N; Filin, Sagi; Aly, Radi; Lande, Tal; Levin, Ilan; Eizenberg, Hanan

    2014-07-01

    Weed/crop classification is considered the main problem in developing precise weed-management methodologies, because both crops and weeds share similar hues. Great effort has been invested in the development of classification models, most based on expensive sensors and complicated algorithms. However, satisfactory results are not consistently obtained due to imaging conditions in the field. We report on an innovative approach that combines advances in genetic engineering and robust image-processing methods to detect weeds and distinguish them from crop plants by manipulating the crop's leaf color. We demonstrate this on genetically modified tomato (germplasm AN-113) which expresses a purple leaf color. An autonomous weed/crop classification is performed using an invariant-hue transformation that is applied to images acquired by a standard consumer camera (visible wavelength) and handles variations in illumination intensities. The integration of these methodologies is simple and effective, and classification results were accurate and stable under a wide range of imaging conditions. Using this approach, we simplify the most complicated stage in image-based weed/crop classification models. © 2013 Society of Chemical Industry.

  4. Pick and Eat Crop Testing: Dwarf Tomato and Pepper as Candidate Space Crops

    Science.gov (United States)

    Wheeler, R. M.; Massa, G. D.; Stutte, G. W.; Spencer, L. E.; Hummerick, M. E.; Sirmons, T.; Douglas, G. L.

    2016-01-01

    Several dwarf tomato and pepper varieties were evaluated under International Space Station (ISS)-simulated growth conditions (22 degrees Centigrade, 50 percent relative humidity, 1500 parts per million CO2, and 300 micromoles per square meter per second of light for 16 hours per day) with the goal of selecting those with the best growth, nutrition, and organoleptic potential for use in a pick and eat salad crop system on ISS and future exploration flights. Testing included six cultivars of tomato (Red Robin, Scarlet Sweet 'N' Neat, Tiny Tim, Mohamed, Patio Princess, and Tumbler) and six cultivars of pepper (Red Skin, Fruit Basket, Cajun Belle, Chablis, Sweet Pickle, and Pompeii). Plants were grown to an age sufficient to produce fruit (up to 106 days for tomato and 109 days for pepper) using Turface (arcillite) potting media with 18-6-8 control-release fertilizer and supplemental nutrient solution beginning around 60-days-age. Tomato fruits were harvested when they showed full red color, beginning around 70-days age and then at weekly intervals thereafter, while peppers were grown until fruits showed color and were harvested twice (first test) and just once at the end of the second test, with the final harvests including colored and green fruit. Plant sizes, yields, and nutritional attributes were measured and used to down-select to three cultivars for each species. In particular, we were interested in cultivars that were short (dwarf) but still produced high yields. Nutritional data included elemental (Ca, Mg, Fe, and K) content, vitamin K, phenolics, lycopene (for tomato), anthocyanin, lutein, and zeaxanthin. The three down-selected cultivars for each species were grown again and the harvested fruit sent to NASA's Johnson Space Center for sensory evaluation, which included overall acceptability, appearance, color intensity, aroma, flavor and texture. The combined data were compared and given weighting factors to rank the cultivars as candidates for testing in

  5. PRODUCTION AND MARKETABILITY OF CONVENTIONAL, SUSTAINABLE AND ORGANIC PRODUCED TOMATOES

    Directory of Open Access Journals (Sweden)

    Dean BAN

    2007-07-01

    Full Text Available Conventional agricultural production is denoted by high levels of chemisation, strait specialised production, high yields and low costs per production unit, however this production causes risky interventions, which could affect negatively on environment and human health Research results indicate possibilities for growing vegetables in alternative systems, less risky for environment with satisfying economic success. The aim of this research was to determine economic success of organic, sustainable and conventional production of tomato in the Mediterranean area of Republic Croatia. Bianual research was conducted during 2002/2003. During vegetation we examined parameters of growth, marketable yields and costs for materials, work and machinery which are used in economic analysis. Economical analysis of tomatoes production indicate worst results in organic production system. Loses in tomatoes organic production were consequences of two main factors: lower marketed yield and equal product price for all three production types. Lower yields in organic production were expected, therefore bad financial results were caused by mainly low market prices, which do not validate quality and food safety. Therefore financial success is preconditioned by higher market validation, which can be obtained through market analysis and product development. Consumer awareness about organic agriculture is still very weak and this point requires further attention. The link between organic agriculture and the environment/nature protection is missing too. The purchase of organic food is influenced by the level of information and knowledge of consumers with reference to these products. Doubts about the truthfulness and significance of some data were raised by main places where organic food is purchased, since an excessive greatest limitations are high prices and a low level of information to consumers. Current standard of life of most Croatian consumers does not permit them to

  6. Chemical oxifertigation through the irrigation of greenhouse hydroponic tomato crop.

    Directory of Open Access Journals (Sweden)

    Freddy Soto-Bravo

    2015-06-01

    Full Text Available   The aim of this study was to evaluate the effect of hydrogen peroxide (H2O2 as an oxygen source in the rhizosphere, in grafted tomato (cv. Durinta/cv Maxifor and using coconut fiber as substrate The study was conducted from 2009 to 2010. Two treatments were used: a control without (H2O2 (T0 and the other with H2O2 (T1 applied in each irrigation. The parameters evaluated were i- fertigation: oxygen concentration ([O2], pH, electrical conductivity (EC, and drainage percentage; ii- growth: basal diameter and plant height; iii- yield and iv- fruit quality: firmness, Brix degrees, dry weight, and pH. The average value of [O2] in the irrigation solution through out the crop cycle increased from 9,92 mg/l at T0 to 12,1 mg/l at T1 (P<0,05, meanwhile in the drained solution the value increased from 8,75 mg/l at T0 to 9,22 mg/l at T1 (P<0,05. Although significant differences (P<0.05 were reached in the [O2] between treatments during some periods of the crop cycle, the [O2] in the T0 did not reach a critical threshold that would affect the proper oxygenation of the roots. Therefore, there was no effect of hydrogen peroxide treatment on the growth, productivity and quality of the fruit.

  7. Occurrence of Faustinus sp. (Coleoptera: Curculionidae in Southeastern Brazil tomato crops

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    Gustavo Dias de Almeida

    2009-12-01

    Full Text Available Occurrence of Faustinus sp. (Coleoptera: Curculionidae in tomato (Solanum lycopersicum L. plantations in the State of Espirito Santo, Brazil, was confirmed through field observations carried out between April 2006 and March 2008. Larvae of Faustinus sp. bore the stems of tomato plants, whereas adults feed on the leaves. Bored stems are then easily broken by the wind, by manual handling or by plant weight itself. Crop rotation and removal of crop residues may help reduce pest population levels.

  8. Altering Conidial Dispersal of Alternaria solani by Modifying Microclimate in Tomato Crop Canopy

    Directory of Open Access Journals (Sweden)

    Prashant Prakash Jambhulkar

    2016-12-01

    Full Text Available Early blight of tomato caused by Alternaria solani, is responsible for severe yield losses in tomato. The conidia survive on soil surface and old dry lower leaves of the plant and spread when suitable climatic conditions are available. Macroclimatic study reveals that highest inoculum concentration of Alternaria spores appeared in May 2012 to 2013 and lowest concentration during January 2012 to 2013. High night temperature positively correlated and significantly (P < 0.01 involved in conidial spore dispersal and low relative humidity (RH displayed significant (P < 0.05 but negative correlation with conidial dispersal. The objective of the study was to modify microclimatic conditions of tomato crop canopy which may hamper conidial dispersal and reduce disease severity. We evaluated effect of marigold intercropping and plastic mulching singly and in consortia on A. solani conidial density, tomato leaf damage and microclimatic parameters as compar to tomato alone (T. Tomato-marigold intercropping–plastic mulching treatment (T + M + P showed 35–39% reduction in disease intensity as compared to tomato alone. When intercropped with tomato, marigold served as barrier to conidial movement and plastic mulching prevented evapotranspiration and reduced the canopy RH that resulted in less germination of A. solani spores. Marigold intercropping and plastic mulching served successfully as physical barrier against conidial dissemination to diminish significantly the tomato foliar damage produced by A. solani.

  9. Effects of Crop Rootzone Non-Pressure Subirrigation on Tomato Physiological Characteristics, Yield, and Quality

    Institute of Scientific and Technical Information of China (English)

    WANG Yan; CAI Huan-jie; CHEN Xin-ming; ZHENG Jian; WANG jian

    2007-01-01

    The purpose of this article is to study the effects of different water elevations of non-pressure subirrigation on some indexes of tomato, including soil water status around crop rootzone, morphological indexes, physiological indexes, photosynthetic indexes, yield, quality, and water use efficiency. With the tomato materials of Dongsheng 1, the irrigation experiment was carried out in the greenhouse, and significance analysis was done on the experiment data through the software of DPS. The results showed that different water elevations, had significant influence on the growth, yield, and quality of tomato. The yield of the 6-cm treatment was the highest, the 3-cm treatment was inferior to the 6-cm treatment, and the 0-cm treatment was the lowest. However, the WUE was 0 cm>3 cm>6 cm. The sugar/acid and soluble protein was the highest under the 0-cm treatment, and the content of ascorbic acid did not decrease considerably. When compared to the 0-cm treatment, the ascorbic acid content of 6-cm and 3-cm treatment increased by 19.2 and 6.8%, respectively. These irrigation methods can satisfy the requirements of tomato growth; different water elevations have different influences on the tomato soil water status around crop rootzone, the physiological characteristics, and yield. It also harmonized the percentage between sugar and acid, increased the content of soluble protein and ascorbic acid, and made tomato more delicious. The irrigation methods can improve the quality of tomato by water control, which is worth promoting in the agricultural production.

  10. Antioxidant activity in selected Slovenian organic and conventional crops

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    Manca KNAP

    2015-12-01

    Full Text Available The demand for organically produced food is increasing. There is widespread belief that organic food is substantially healthier and safer than conventional food. According to literature organic food is free of phytopharmaceutical residues, contain less nitrates and more antioxidants. The aim of the present study was to verify if there are any differences in the antioxidant activity between selected Slovenian organic and conventional crops. Method of DPPH (2,2-diphenyl-1-picryhydrazyl was used to determine the antioxidant activity of 16 samples from organic and conventional farms. The same varieties of crops were analysed. DPPH method was employed to measure the antioxidant activity of polar antioxidants (AAp and antioxidant activity of fraction in ethyl acetate soluble antioxidants (EA AA. Descriptive statistics and variance analysis were used to describe differences between farming systems. Estimated differences between interactions for the same crop and different farming practice were mostly not statistically significant except for the AAp for basil and beetroot. Higher statistically significant values were estimated for conventional crops. For the EA AA in broccoli, cucumber, rocket and cherry statistically significant higher values were estimated for organic production.

  11. 7 CFR 457.128 - Guaranteed production plan of fresh market tomato crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... the insured crop directly to consumers without the intervention of an intermediary such as a... Special Provisions. The price election you choose for each type must have the same percentage relationship...: (A) Grown fresh market tomatoes for commercial sales; or (B) Participated in the management of...

  12. Soil Organic Carbon Pools Under Switchgrass Grown as a Bioenergy Crop Compared to Other Conventional Crops

    Institute of Scientific and Technical Information of China (English)

    F.G.DOU; F.M.HONS; W.R.OCUMPAUGH; J.C.READ; M.A.HUSSEY; J.P.MUIR

    2013-01-01

    Switchgrass (Panicum virgatum L.) has been proposed as a sustainable bioenergy crop because of its high yield potential,adaptation to marginal sites,and tolerance to water and nutrient limitations.A better understanding of the potential effects of biomass energy crop production practices on soil biological properties and organic matter dynamics is critical to its production.Our objective was to evaluate changes in C pools under a warm-season perennial switchgrass in different soils compared to typically-grown crops collected at College Station,Dallas,and Stephenville,TX in February 2001.Sampling depths were 0-5,5-15,and 15-30 cm.Switchgrass increased soil organic C (SOC),soil microbial biomass C (SMBC),mineralizable C,and particulate organic matter C (POM-C) compared to conventional cropping systems.Soil C concentrations were in the order:long-term coastal bermudagrass [Cynodon dactylon (L.) Pers.] > switchgrass or kleingrass (Panicum coloratum L.) planted in 1992 > switchgrass 1997 > conventional cropping systems.Soil C concentrations tended to increase with increasing clay content.Greater microbial biomass C followed the order of Dallas >College Station > Stephenville,and ranged from approximately 180 mg C kg-1 soil at Stephenville to 1900 mg C kg-1 soil at Dallas.Particulate organic C was more sensitive than other fractions to management,increasing as much as 6-fold under long-term coastal bermudagrass compared to conventional cropping systems.Our study indicated that conversion of conventional cropping systems into switchgrass production can sequestrate more SOC and improve soil biological properties in the southern USA.

  13. Metabolomic fingerprinting employing DART-TOFMS for authentication of tomatoes and peppers from organic and conventional farming.

    Science.gov (United States)

    Novotná, H; Kmiecik, O; Gałązka, M; Krtková, V; Hurajová, A; Schulzová, V; Hallmann, E; Rembiałkowska, E; Hajšlová, J

    2012-01-01

    The rapidly growing demand for organic food requires the availability of analytical tools enabling their authentication. Recently, metabolomic fingerprinting/profiling has been demonstrated as a challenging option for a comprehensive characterisation of small molecules occurring in plants, since their pattern may reflect the impact of various external factors. In a two-year pilot study, concerned with the classification of organic versus conventional crops, ambient mass spectrometry consisting of a direct analysis in real time (DART) ion source and a time-of-flight mass spectrometer (TOFMS) was employed. This novel methodology was tested on 40 tomato and 24 pepper samples grown under specified conditions. To calculate statistical models, the obtained data (mass spectra) were processed by the principal component analysis (PCA) followed by linear discriminant analysis (LDA). The results from the positive ionisation mode enabled better differentiation between organic and conventional samples than the results from the negative mode. In this case, the recognition ability obtained by LDA was 97.5% for tomato and 100% for pepper samples and the prediction abilities were above 80% for both sample sets. The results suggest that the year of production had stronger influence on the metabolomic fingerprints compared with the type of farming (organic versus conventional). In any case, DART-TOFMS is a promising tool for rapid screening of samples. Establishing comprehensive (multi-sample) long-term databases may further help to improve the quality of statistical classification models.

  14. Optimization of crop productivity in tomato using induced mutations in the florigen pathway.

    Science.gov (United States)

    Park, Soon Ju; Jiang, Ke; Tal, Lior; Yichie, Yoav; Gar, Oron; Zamir, Dani; Eshed, Yuval; Lippman, Zachary B

    2014-12-01

    Naturally occurring genetic variation in the universal florigen flowering pathway has produced major advancements in crop domestication. However, variants that can maximize crop yields may not exist in natural populations. Here we show that tomato productivity can be fine-tuned and optimized by exploiting combinations of selected mutations in multiple florigen pathway components. By screening for chemically induced mutations that suppress the bushy, determinate growth habit of field tomatoes, we isolated a new weak allele of the florigen gene SINGLE FLOWER TRUSS (SFT) and two mutations affecting a bZIP transcription factor component of the 'florigen activation complex' (ref. 11). By combining heterozygous mutations, we pinpointed an optimal balance of flowering signals, resulting in a new partially determinate architecture that translated to maximum yields. We propose that harnessing mutations in the florigen pathway to customize plant architecture and flower production offers a broad toolkit to boost crop productivity.

  15. NATIVE LARVAL PARASITOIDS ASSOCIATED WITH TUTA ABSOLUTA (MEYRICK) IN GREENHOUSE TOMATO CROPS OF SOUTHERN SARDINIA.

    Science.gov (United States)

    Nannini, M; Atzori, F; Pisci, R; Sanna, F

    2014-01-01

    Since its first detection in Sardinia (Italy), Tuta absoluta (Meyrick) has been reported as a major pest of greenhouse tomatoes. In recent years, however, a tendency toward a progressive reduction of tomato borer infestation levels has been observed. The reasons behind this decline are probably diverse, including both the increase in growers' ability to manage the pest and the adaptation of native predators and parasitoids to the new prey/host. In order to assess the species composition of the parasitoid complex associated with T. absoluta larvae in Sardinian greenhouse tomatoes, a two-year (2010/11) survey was conducted in one of the island's major horticultural areas (Pula, Cagliari). An estimate of the levels of parasitism caused by native wasps was also carried out. The occurrence of larval parasitoids of T. absoluta was detected in approximately half of the crops monitored. The average parasitism rate recorded in tomato plants infested by tomato borer larvae was 1.3%. Moreover, while no parasitism was observed in 76.4% of the samples collected, only 3.0% of the samples showed a parasitism rate exceeding 10%. The highest rates were recorded in spring and summer, with a peak in July (4.8%). Of 159 adult parasitoids recovered from infested leaves, 62.9% were found to belong to a Necremnus sp. near artynes (Walker), 34.6% to a Necremnus sp. near tidius (Walker), and 2.5% to Neochrysocharis formosa (Westwood) (Hymenoptera: Eulophidae). These results show that some native parasitoids have adapted to T. absoluta in the study area. Although parasitoid abundance in tomato crops appeared to be low, their contribution for the control of tomato borer infestation could possibly be enhanced through the application of conservation biological control measures.

  16. 77 FR 22467 - Common Crop Insurance Regulations; Fresh Market Tomato (Dollar Plan) Crop Provisions

    Science.gov (United States)

    2012-04-16

    ... collection of administrative fees from limited resource farmers. FCIC believes this waiver helps to ensure... have a significant economic impact on the quality of the human environment, health, or safety... tomatoes and the U.S. Standards for Grades of Fresh Tomatoes, and the AMS Federal Marketing Order (FMO 966...

  17. Qualitative and nutritional differences in processing tomatoes grown under commercial organic and conventional production systems.

    Science.gov (United States)

    Barrett, D M; Weakley, C; Diaz, J V; Watnik, M

    2007-11-01

    Organically grown products experienced a doubling in percent penetration of organic sales into retail markets during the period from 1997 to 2003; however, there is still a debate over the perceived quality advantage of organically grown fruits and vegetables. In a study focusing on commercial production of processing tomatoes, samples were analyzed from 4 growers with matched organic and conventional fields. For the 4 growers studied, individual analysis of variance results indicated that tomato juice prepared from organically produced tomatoes on some farms was significantly higher in soluble solids ( degrees Brix), higher in consistency, and titratable acidity, but lower in red color, ascorbic acid, and total phenolics content in the microwaved juice. Results were significantly different among specific growers, and this may be attributed to differences in soil type and soil nutrients, tomato cultivar, environmental conditions, or other production-related factors. Higher levels of soluble solids, titratable acidity, and consistency are desirable for the production of tomato paste, in that tomatoes with these attributes may be more flavorful and require less thermal treatment. This has the potential to result both in cost savings from less energy required in paste manufacture and potentially a higher quality product due to less thermal degradation of color, flavor, and nutrients. Future work may involve a larger number of commercial growers and correlation to controlled university research plots.

  18. Population Modeling Approach to Optimize Crop Harvest Strategy. The Case of Field Tomato

    Directory of Open Access Journals (Sweden)

    Maarten L. A. T. M. Hertog

    2017-04-01

    Full Text Available In this study, the aim is to develop a population model based approach to optimize fruit harvesting strategies with regard to fruit quality and its derived economic value. This approach was applied to the case of tomato fruit harvesting under Vietnamese conditions. Fruit growth and development of tomato (cv. “Savior” was monitored in terms of fruit size and color during both the Vietnamese winter and summer growing seasons. A kinetic tomato fruit growth model was applied to quantify biological fruit-to-fruit variation in terms of their physiological maturation. This model was successfully calibrated. Finally, the model was extended to translate the fruit-to-fruit variation at harvest into the economic value of the harvested crop. It can be concluded that a model based approach to the optimization of harvest date and harvest frequency with regard to economic value of the crop as such is feasible. This approach allows growers to optimize their harvesting strategy by harvesting the crop at more uniform maturity stages meeting the stringent retail demands for homogeneous high quality product. The total farm profit would still depend on the impact a change in harvesting strategy might have on related expenditures. This model based harvest optimisation approach can be easily transferred to other fruit and vegetable crops improving homogeneity of the postharvest product streams.

  19. Efficacy of conventional fungicides in controlling tomato grey mold

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    A. Mavandadi

    2016-02-01

    Full Text Available One of the important diseases of tomato plants is grey mold, which is caused by Botrytis cinerea. In this research, the effects of 10 fungicides on mycelial growth and conidia germination of B. cinerea were investigated. Results showed that carbendazim with a median inhibitory concentration (IC50 value of 0.1 mg/L had the highest inhibitory effect and bordeaux mixture with an IC50 value of 1096.9 mg/L had the lowest inhibitory effect. Benomyl with an IC50 value of 0.04 mg/L had the most visual effects, after 3 and 7 days, on mycelial growth of B. cinerea. The effect of fungicides on preventing fruit wound development caused by B. cinerea was investigated, too. In benomyl treatment, wound diameter on the fruits was 0.73 mm and in copper oxychloride treatment was 6.16 mm, showing the highest and the lowest effective fungicides in the prevention of wound expansion. Different strains of the fungus were collected from various areas in Isfahan province and the effect of the fungicides on their conidial germination was evaluated. Results revealed that the collected isolates had different susceptibility to the 10 tested fungicides.

  20. Evaluation of two composts for the improvement of crop yield using tomato (Lycopersicon esculentum as test crop

    Directory of Open Access Journals (Sweden)

    Fawole Oluyemisi B.

    2016-01-01

    Full Text Available In search of a more environmentally friendly alternative to the use of chemical fertilizers, a study was conducted to evaluate the use of compost for improved crop productivity. We compared the succession of microorganisms in the compost heaps using hot bed method of composting. They contained grass clippings, sawdust, NPK fertilizer, ashes, corn cobs, bean chaff, vegetable stalks, newspaper shreds and soil arranged in layers in a round structure. Poultry dropping was the organic nitrogen source of one heap while pig waste was used for the other heap. Samples were taken weekly and analyzed using soil dilution method for isolation of moulds on potato dextrose agar medium. The qualities of composts after eight weeks were evaluated by performance and yield of tomato crops. Eleven fungal isolates were obtained in compost containing poultry dropping and nine fungal isolates were obtained from compost containing pig manure. The predominant mycoflora of poultry dropping compost at 3 weeks of composting was Fusarium pallidoroseum (23.08% while Aspergillus fumigatus (38.96% dominated compost containing pig waste. Fungi isolated from the composts included cellulolytic fungi like Chaetomium sp. and Phoma sp. Soil amended with both composts improved the growth and yield of tomato crop significantly. It was concluded that compost containing poultry droppings was richer and therefore encouraged higher microbial activity than compost containing pig waste. Knowledge of the microbial succession during composting and conditions required could further be employed to enhance composting.

  1. Clavibacter michiganensis subsp. michiganensis, Bacterial Canker of Tomato: 1. Conventional and Molecular Identification

    Directory of Open Access Journals (Sweden)

    Svetlana Milijašević

    2006-01-01

    Full Text Available In May 2006, tomato leaves with watersoaked areas between leaf veins were detected in glasshouses in Padinska Skela. The early-stage symptoms were followed by rapid desiccation to white and pale brown necrosis and subsequently by wilting of entire tomato plants. In June 2006, symptoms of bacterial canker and wilt were also recorded in manygreenhouses in the Jablanica County in southern Serbia.Samples of diseased tomato plants were collected from several tomato-growing regions in Serbia in order to identify the causal agent using conventional identification methods (isolation, pathogenicity and bacteriological determinative tests. Another objectiveof this study was to confirm the identity of isolated bacterial strains by polymerase chain reaction (PCR method.According to the morphological characteristics observed on NA and NBY media, biochemical characteristics, hypersensitive response in four-o’clock plant leaves and pathogenicity test on tomato seedlings, the investigated strains were identified as C. michiganensis subsp. michiganensis. To confirm the identity of isolated strains two PCR protocols were used. Amplification of expected length DNA fragments, 614 bp and 270 bp, respectively, confirmed that the investigated strains belonged to C. michiganensis subsp. michiganensis.

  2. Begomovirus diversity in tomato crops and weeds in Ecuador and the detection of a recombinant isolate of rhynchosia golden mosaic Yucatan virus infecting tomato.

    Science.gov (United States)

    Paz-Carrasco, Lenin C; Castillo-Urquiza, Gloria P; Lima, Alison T M; Xavier, Cesar A D; Vivas-Vivas, Leticia M; Mizubuti, Eduardo S G; Zerbini, F Murilo

    2014-08-01

    Viral diseases caused by begomoviruses are of economic importance due to their adverse effects on the production of tropical and subtropical crops. In Ecuador, despite reports of significant infestations of Bemisia tabaci in the late 1990s, only very recently has a begomovirus, tomato leaf deformation virus (ToLDeV, also present in Peru), been reported in tomato. ToLDeV is the first monopartite begomovirus discovered that originated in the Americas, and its presence in Ecuador highlights the need for a wider survey of tomato-infecting begomoviruses in this country. Tomato and weed samples were collected in 2010 and 2011 in six provinces of Ecuador, and begomovirus genomes were cloned and sequenced using a rolling-circle-amplification-based approach. Most tomato samples from the provinces of Guayas, Loja, Manabi and Santa Elena were infected with tomato leaf deformation virus (ToLDeV). One sample from Manabi had a triple infection with ToLDeV, rhynchosia golden mosaic Yucatan virus (RhGMYuV) and an isolate that was a recombinant between the two. A new begomovirus was detected in another tomato sample from Manabi. Samples of Rhynchosia sp. from the provinces of Guayas and Manabi were infected by RhGMYuV. These results indicate not only the prevalence of ToLDeV in tomato in Ecuador but also the presence of other viruses, albeit at a much lower frequency.

  3. A new mutant genetic resource for tomato crop improvement by TILLING technology

    Directory of Open Access Journals (Sweden)

    Sozio Giovanni

    2010-03-01

    Full Text Available Abstract Background In the last decade, the availability of gene sequences of many plant species, including tomato, has encouraged the development of strategies that do not rely on genetic transformation techniques (GMOs for imparting desired traits in crops. One of these new emerging technology is TILLING (Targeting Induced Local Lesions In Genomes, a reverse genetics tool, which is proving to be very valuable in creating new traits in different crop species. Results To apply TILLING to tomato, a new mutant collection was generated in the genetic background of the processing tomato cultivar Red Setter by treating seeds with two different ethylemethane sulfonate doses (0.7% and 1%. An associated phenotype database, LycoTILL, was developed and a TILLING platform was also established. The interactive and evolving database is available online to the community for phenotypic alteration inquiries. To validate the Red Setter TILLING platform, induced point mutations were searched in 7 tomato genes with the mismatch-specific ENDO1 nuclease. In total 9.5 kb of tomato genome were screened and 66 nucleotide substitutions were identified. The overall mutation density was estimated and it resulted to be 1/322 kb and 1/574 kb for the 1% EMS and 0.7% EMS treatment respectively. Conclusions The mutation density estimated in our collection and its comparison with other TILLING populations demonstrate that the Red Setter genetic resource is suitable for use in high-throughput mutation discovery. The Red Setter TILLING platform is open to the research community and is publicly available via web for requesting mutation screening services.

  4. Adaptive management of irrigation and crops' biodiversity: a case study on tomato

    Science.gov (United States)

    De Lorenzi, Francesca; Alfieri, Silvia Maria; Basile, Angelo; Bonfante, Antonello; Monaco, Eugenia; Riccardi, Maria; Menenti, Massimo

    2013-04-01

    We have assessed the impacts of climate change and evaluated options to adapt irrigation management in the face of predicted changes of agricultural water demand. We have evaluated irrigation scheduling and its effectiveness (versus crop transpiration), and cultivars' adaptability. The spatial and temporal variations of effectiveness and adaptability were studied in an irrigated district of Southern Italy. Two climate scenarios were considered: reference (1961-90) and future (2021-2050) climate, the former from climatic statistics, and the latter from statistical downscaling of general circulation models (AOGCM). Climatic data consist of daily time series of maximum and minimum temperature, and daily rainfall on a grid with a spatial resolution of 35 km. The work was carried out in the Destra Sele irrigation scheme (18.000 ha. Twenty-five soil units were identified and their hydrological properties were determined (measured or estimated from texture through pedo-transfer functions). A tomato crop, in a rotation typical of the area, was considered. A mechanistic model of water flow in the soil-plant-atmosphere system (SWAP) was used to study crop water requirements and water consumption. The model was calibrated and validated in the same area for many different crops. Tomato crop input data and model parameters were estimated on the basis of scientific literature and assumed to be generically representative of the species. Simulations were performed for reference and future climate, and for different irrigation scheduling options. In all soil units, six levels of irrigation volumes were applied: full irrigation (100%), deficit irrigation (80%, 60%, 40%, 20%), no irrigation. From simulation runs, indicators of soil water availability were calculated, moreover the marginal increases of transpiration per unit of irrigation volume, i.e. the effectiveness of irrigation (ΔT/I), were computed, in both climate scenarios. Indicators and marginal increases were used to

  5. Effect of organic and conventional crop rotation, fertilization, and crop protection practices on metal contents in wheat (Triticum aestivum).

    Science.gov (United States)

    Cooper, Julia; Sanderson, Roy; Cakmak, Ismail; Ozturk, Levent; Shotton, Peter; Carmichael, Andrew; Haghighi, Reza Sadrabadi; Tetard-Jones, Catherine; Volakakis, Nikos; Eyre, Mick; Leifert, Carlo

    2011-05-11

    The effects of organic versus conventional crop management practices (crop rotation, crop protection, and fertility management strategies) on wheat yields and grain metal (Al, Cd, Cu, Ni, Pb, and Zn) concentrations were investigated in a long-term field trial. The interactions between crop management practices and the season that the crop was grown were investigated using univariate and redundancy analysis approaches. Grain yields were highest where conventional fertility management and crop protection practices were used, but growing wheat after a previous crop of grass/clover was shown to partially compensate for yield reductions due to the use of organic fertility management. All metals except for Pb were significantly affected by crop management practices and the year that the wheat was grown. Grain Cd and Cu levels were higher on average when conventional fertility management practices were used. Al and Cu were higher on average when conventional crop protection practices were used. The results demonstrate that there is potential to manage metal concentrations in the diet by adopting specific crop management practices shown to affect crop uptake of metals.

  6. Using NDVI and guided sampling to develop yield prediction maps of processing tomato crop

    Energy Technology Data Exchange (ETDEWEB)

    Fortes, A.; Henar Prieto, M. del; García-Martín, A.; Córdoba, A.; Martínez, L.; Campillo, C.

    2015-07-01

    The use of yield prediction maps is an important tool for the delineation of within-field management zones. Vegetation indices based on crop reflectance are of potential use in the attainment of this objective. There are different types of vegetation indices based on crop reflectance, the most commonly used of which is the NDVI (normalized difference vegetation index). NDVI values are reported to have good correlation with several vegetation parameters including the ability to predict yield. The field research was conducted in two commercial farms of processing tomato crop, Cantillana and Enviciados. An NDVI prediction map developed through ordinary kriging technique was used for guided sampling of processing tomato yield. Yield was studied and related with NDVI, and finally a prediction map of crop yield for the entire plot was generated using two geostatistical methodologies (ordinary and regression kriging). Finally, a comparison was made between the yield obtained at validation points and the yield values according to the prediction maps. The most precise yield maps were obtained with the regression kriging methodology with RRMSE values of 14% and 17% in Cantillana and Enviciados, respectively, using the NDVI as predictor. The coefficient of correlation between NDVI and yield was correlated in the point samples taken in the two locations, with values of 0.71 and 0.67 in Cantillana and Enviciados, respectively. The results suggest that the use of a massive sampling parameter such as NDVI is a good indicator of the distribution of within-field yield variation. (Author)

  7. Compatibility of entomopathogenic nematodes (Nematoda: Rhabditida with insecticides used in the tomato crop

    Directory of Open Access Journals (Sweden)

    Paulo Henrique de Siqueira Sabino

    2014-07-01

    Full Text Available Entomopathogenic nematodes (EPNs are agents that can be used for the biological control of pests associated with insecticides in a tank mix. Compatibility studies need to be conducted to analyze which products are compatible with nematodes. The aim of this work was to evaluate the compatibility between EPNs and the insecticides that are most used on the tomato crop, and to correlate the toxicological classification of the chemical products with two species of EPNs that have the potential to control tomato leaf miner, Tuta absoluta. Among the products tested, Certero (triflumuron, Decis (deltamethrin, Previcur (dimethylamino-propyl, Ampligo (lambdacyhalothrin + chlorantranilprole, Premio (clorantranilprole, Engeo Pleno (thiamethoxam + lambda-cyhalothrin were compatible (IOBC class 1 with both nematode species.

  8. Pollination deficit in open-field tomato crops (Solanum lycopersicum L., Solanaceae in Rio de Janeiro state, Southeast Brazil

    Directory of Open Access Journals (Sweden)

    Mariana Scaramussa Deprá

    2014-02-01

    Full Text Available More than 70% of world’s crops benefit from biotic pollination, and bees are their main pollinators. Despite the fact that some of these insects have been broadly studied, understanding the interactions between plant crops and their pollinators with a local scale approach is necessary when aiming to apply proper protective and management measures to pollinators and their respective crops. In this context, we analyzed the pollination status of open-field tomato crops (Solanum lycopersicum L., regarding fruit-set, visitation rate and the quality of fruits. We recorded the formation of fruits through spontaneous self-pollination and open-pollination, and the occurrence of pollinators in 24 areas of open-field tomato crops. We performed experiments of apomixis, spontaneous self-pollination, manual cross pollination and supplemental cross pollination (simulating the pollinator behavior in a greenhouse. The fruit quality was evaluated according to circumference, weight, volume and number of seeds. Higher production of fruits after open-pollination compared to spontaneous self-pollination indicates the importance of pollinators to increment productivity of S. lycopersicum in the study area. The circumference and the number of seeds from tomatoes of the greenhouse plantation did not differ between spontaneous self-pollination and the manual cross pollination. In the open-field crops the number of seeds was higher for fruits resulting from open-pollination. Our results indicate that the importance of bees is mainly related to the increase in fruit production, thus incrementing the productivity of tomato crops.

  9. Pollination deficit in open-field tomato crops (Solanum lycopersicum L., Solanaceae in Rio de Janeiro state, Southeast Brazil

    Directory of Open Access Journals (Sweden)

    Maria Cristina Gaglianone

    2013-10-01

    Full Text Available More than 70% of world’s crops benefit from biotic pollination, and bees are their main pollinators. Despite the fact that some of these insects have been broadly studied, understanding the interactions between plant crops and their pollinators with a local scale approach is necessary when aiming to apply proper protective and management measures to pollinators and their respective crops. In this context, we analyzed the pollination status of open-field tomato crops (Solanum lycopersicum L., regarding fruit-set, visitation rate and the quality of fruits. We recorded the formation of fruits through spontaneous self-pollination and open-pollination, and the occurrence of pollinators in 24 areas of open-field tomato crops. We performed experiments of apomixis, spontaneous self-pollination, manual cross pollination and supplemental cross pollination (simulating the pollinator behavior in a greenhouse. The fruit quality was evaluated according to circumference, weight, volume and number of seeds. Higher production of fruits after open-pollination compared to spontaneous self-pollination indicates the importance of pollinators to increment productivity of S. lycopersicum in the study area. The circumference and the number of seeds from tomatoes of the greenhouse plantation did not differ between spontaneous self-pollination and the manual cross pollination. In the open-field crops the number of seeds was higher for fruits resulting from open-pollination. Our results indicate that the importance of bees is mainly related to the increase in fruit production, thus incrementing the productivity of tomato crops.

  10. Soil and plant nitrogen dynamics of a tomato crop under different fertilization strategies

    DEFF Research Database (Denmark)

    Doltra, Jordi; Muñoz, P; Antón, A

    2010-01-01

    A field experiment was conducted in 2007 to investigate the effects of the N fertilizer source on the soil and plant N dynamics of a tomato crop grown in a sandy loam soil. The fertilization treatments were: mineral N-fertilization applied by fertigation (TM); organic N-fertilization (TO) with co......A field experiment was conducted in 2007 to investigate the effects of the N fertilizer source on the soil and plant N dynamics of a tomato crop grown in a sandy loam soil. The fertilization treatments were: mineral N-fertilization applied by fertigation (TM); organic N-fertilization (TO......) with compost obtained from the organic fraction of urban waste (OFUW); and a combined treatment (TC) with half organic and half mineral N. Compost was incorporated in November 2006. Plants were drip irrigated with well water. The nitrate concentrations in the irrigation solutions were determined on a weekly....... The model was calibrated using data from a previous experiment. No differences between treatments were observed with respect to yield or N content in marketable fruits. The amount of N left in the field at the end of the cropping period was significantly lower in TO than in TC and TM. Simulated plant growth...

  11. Emissions of nitrous oxide from arable organic and conventional cropping systems on two soil types

    DEFF Research Database (Denmark)

    Chirinda, N.; Carter, Mette Sustmann; Albert, Kristian Rost

    2010-01-01

    . The main objective of this study was to compare nitrous oxide (N2O) emissions from soil under winter wheat (Triticum aestivum L.) within three organic and one conventional cropping system that differed in type of fertilizer, presence of catch crops and proportion of N2-fixing crops. The study...

  12. Natural Occurrence of Tomato leaf curl New Delhi virus in Iranian Cucurbit Crops

    Directory of Open Access Journals (Sweden)

    Sara Yazdani-Khameneh

    2016-06-01

    Full Text Available The main areas for field-grown vegetable production in Iran were surveyed during the years of 2012–2014 to determine the occurrence of begomoviruses infecting these crops. A total of 787 leaf samples were collected from vegetables and some other host plants showing virus-like symptoms and tested by an enzyme-linked immunosorbent assay (ELISA using polyclonal antibodies produced against Tomato yellow leaf curl virus (TYLCV. According to the ELISA results, 81 samples (10.3% positively reacted with the virus antibodies. Begomovirus infections were confirmed by polymerase chain reaction (PCR using previously described TYLCV-specific primer pair TYLCV-Sar/TYLCV-Isr or universal primer pair Begomo-F/Begomo-R. The PCR tests using the primer pair TYLCV-Sar/TYLCV-Isr resulted in the amplification of the expected fragments of ca. 0.67-kb in size for ELISA-positive samples tested from alfalfa, pepper, spinach and tomato plants, confirming the presence of TYLCV. For one melon sample, having a week reaction in ELISA and no reaction in PCR using TYLCV-specific primers, the PCR reaction using the primer pair Begomo-F/Begomo-R resulted in the amplification fragments of the expected size of ca. 2.8 kb. The nucleotide sequences of the DNA amplicons derived from the isolate, Kz-Me198, were determined and compared with other sequences available in GenBank. BLASTN analysis confirmed the begomovirus infection of the sample and showed 99% identities with Tomato leaf curl New Delhi virus (ToLCNDV; phylogenetic analysis supported the results of the database searches. This study reports the natural occurrence of TYLCV in different hosts in Iran. Our results also reveal the emergence of ToLCNDV in Iranian cucurbit crops.

  13. Performance of Conventional Activated Sludge to Remove Nitrogen Compounds from Tomato Factory Wastewater

    Directory of Open Access Journals (Sweden)

    Meghdad Pirsaheb

    2012-10-01

    Full Text Available Today discharge of raw or treated wastewater containing nutrients (nitrogen and phosphorus compounds to the surface water causing an Utrification phenomenon, will be due to excessive growth of algae in the receptive water source. Each of the of wastewater treatment system, providing principled design and operation can be reduced nutrients to standard level [1]. The purpose of this study was to evaluate the efficacy of conventional activated sludge systems to remove nitrogen compounds from wastewater of Kermanshah Rojintak tomato factory and comparison of the final effluent quality with discharge standards to water resource and reuse it in agricultural irrigation in term of nitrogen compounds are considered.

  14. Microbial responses and nitrous oxide emissions during wetting and drying of organically and conventionally managed soil under tomatoes

    Science.gov (United States)

    Burger, M.; Jackson, L.E.; Lundquist, E.J.; Louie, D.T.; Miller, R.L.; Rolston, D.E.; Scow, K.M.

    2005-01-01

    The types and amounts of carbon (C) and nitrogen (N) inputs, as well as irrigation management are likely to influence gaseous emissions and microbial ecology of agricultural soil. Carbon dioxide (CO2) and nitrous oxide (N2O) efflux, with and without acetylene inhibition, inorganic N, and microbial biomass C were measured after irrigation or simulated rainfall in two agricultural fields under tomatoes (Lycopersicon esculentum). The two fields, located in the California Central Valley, had either a history of high organic matter (OM) inputs ("organic" management) or one of low OM and inorganic fertilizer inputs ("conventional" management). In microcosms, where short-term microbial responses to wetting and drying were studied, the highest CO2 efflux took place at about 60% water-filled pore space (WFPS). At this moisture level, phospholipid fatty acids (PLFA) indicative of microbial nutrient availability were elevated and a PLFA stress indicator was depressed, suggesting peak microbial activity. The highest N 2O efflux in the organically managed soil (0.94 mg N2O-N m-2 h-1) occurred after manure and legume cover crop incorporation, and in the conventionally managed soil (2.12 mg N2O-N m-2 h-1) after inorganic N fertilizer inputs. Elevated N2O emissions occurred at a WFPS >60% and lasted <2 days after wetting, probably because the top layer (0-150 mm) of this silt loam soil dried quickly. Therefore, in these cropping systems, irrigation management might control the duration of elevated N2O efflux, even when C and inorganic N availability are high, whereas inorganic N concentrations should be kept low during times when soil moisture cannot be controlled.

  15. Partial Rootzone Drying: Changing Alternation Frequency at Different Phenological Stages and Impact on Tomato Crop

    Directory of Open Access Journals (Sweden)

    N. Affi

    2014-01-01

    Full Text Available The goal of the current work was to assess the effect of different alternation frequency applied at different phenological stages on physiological parameters of a Partial rootzone drying (PRD irrigated tomato crop. Three treatments were applied. Besides the control irrigated at 100% of its water requirements, T3 and T4 are both treatments that received 50% of water requirements and that were irrigated by PRD strategy. Crop cycle was divided into three stages: S1 lasted from transplanting to 6th truss flowering, S2: the period separating the 6 th truss flowering and the 2 nd truss harvest, S3: lasted for the remaining crop cycle period beginning at 2nd truss harvest. While T4 was alternated every 10 days similarly, T3 was alternated every 14 days, 12 days and 10 days during S1, S2 and S3, respectively. T3 maximum daily shrinkage (MDS was 70% higher than T4 showing that the later is more efficient than the former. As far as stomatal conductance (Cs and leaf water potential (Ψl, results show that both PRD treatments were affected by stress without noticing any statistical differences in terms of those parameters. The control presented the highest Cs and Ψl levels during the whole crop cycle and the lowest water use efficiency (WUE.

  16. Crop and soil-water stress coefficients of tomato in the glass-greenhouse conditions

    Directory of Open Access Journals (Sweden)

    Z. Razmi

    2011-12-01

    Full Text Available In order to determine the actual evapotranspiration of tomato in the greenhouse, crop and soil-water stress coefficients were surveyed. To compare the actual evapotranspiration at different irrigation intervals (1, 2, 4, 6 and 8-day, a completely randomized blocks design with four replications was performed. The present study was carried out in a greenhouse covered by 4 mm thick glass. Maximum and minimum temperatures and solar radiation were measured inside the greenhouse once in 24 h. Relative humidity was measured in the greenhouse once in 2 h. Microclimate data were measured in a metrological station, 100 m from the greenhouse, simultaneously, at outside the greenhouse. Reference crop evapotranspiration was calculated by FAO Penman-Monteith method for inside and outside of the greenhouse. Results indicated that the reference evapotranspiration in the inside of the greenhouse was 73% of outside the greenhouse. The actual evapotranspiration of tomato for inside of the greenhouse was determined by using the water balance method. By using the pergeometer and albidometer data, the crop coefficient for inside the greenhouse at three different stages (development, mid, and end of growth was determined as 0.85, 1.0 and 0.77, respectively. Soil-water stress coefficient, with readily available coefficient of 0.7, was determined to be in the range of 0.53 to 0.98 for all the treatments. This coefficient was 0.88 for water-stressed 4-day treatment, and reduced to 0.72 for 8-day treatment.

  17. Carbon footprints of crops from organic and conventional arable crop rotations – using a life cycle assessment approach

    DEFF Research Database (Denmark)

    Knudsen, Marie Trydeman; Meyer-Aurich, A; Olesen, Jørgen E

    2014-01-01

    organic arable crop rotations with different sources of N supply. Data from long-term field experiments at three different locations in Denmark were used to analyse three different organic cropping systems (‘Slurry’, ‘Biogas’ and ‘Mulching’), one conventional cropping system (‘Conventional’) and a “No...... input” system as reference systems. The ‘Slurry’ and ‘Conventional’ rotations received slurry and mineral fertilizer, respectively, whereas the ‘No input’ was unfertilized. The ‘Mulching’ and ‘Biogas’ rotations had one year of grass-clover instead of a faba bean crop. The grass-clover biomass...... was incorporated in the soil in the ‘Mulching’ rotation and removed and used for biogas production in the ‘Biogas’ rotation (and residues from biogas production were simulated to be returned to the field). A method was suggested for allocating effects of fertility building crops in life cycle assessments...

  18. Soil and plant nitrogen dynamics of a tomato crop under different fertilization strategies

    DEFF Research Database (Denmark)

    Doltra, Jordi; Muñoz, P; Antón, A

    2010-01-01

    ) with compost obtained from the organic fraction of urban waste (OFUW); and a combined treatment (TC) with half organic and half mineral N. Compost was incorporated in November 2006. Plants were drip irrigated with well water. The nitrate concentrations in the irrigation solutions were determined on a weekly......A field experiment was conducted in 2007 to investigate the effects of the N fertilizer source on the soil and plant N dynamics of a tomato crop grown in a sandy loam soil. The fertilization treatments were: mineral N-fertilization applied by fertigation (TM); organic N-fertilization (TO...... basis. Soil samples were taken before planting and at harvest from depths of up to 90 cm in order to determine moisture and N-NO3- levels. The estimated amounts of total N-NO3- available in the different treatments, including the initial content in the 0-90 cm soil layer, were 560 (TC), 570 (TO) and 610...

  19. Productivity of organic and conventional arable cropping systems in long-term experiments in Denmark

    DEFF Research Database (Denmark)

    Shah, Ambreen; Askegaard, Margrethe; Rasmussen, Ilse Ankjær

    2017-01-01

    A field experiment comparing different arable crop rotations was conducted in Denmark during 1997–2008 on three sites varying in climatic conditions and soil types, i.e. coarse sand (Jyndevand), loamy sand (Foulum), and sandy loam (Flakkebjerg). The crop rotations followed organic farm management......, and from 2005 also conventional management was included for comparison. Three experimental factors were included in the experiment in a factorial design: 1) crop rotation (organic crop rotations varying in use of whole-year green manure (O1 and O2 with a whole-year green manure, and O4 without......), and a conventional system without green manure (C4)), 2) catch crop (with and without), and 3) manure (with and without). The experiment consisted of three consecutive cycles using four-course rotations with all crops present every year, i.e. 1997–2000 (1st cycle), 2001–2004 (2nd cycle), and 2005–2008 (3rd cycle...

  20. Conventional crop management effects on soil organic matter characteristics

    OpenAIRE

    2003-01-01

    International audience; A Mediterranean soil affected by long-term crop management systems was evaluated to identify the best indicators of its quality. Selected characteristics included total soil organic carbon (TOC), light fraction carbon (LFC), acid hydrolyzable and water-soluble carbohydrates carbon (AHG-C and WSC-C), phenolic carbon (PC), mineralizable carbon (Q), microbial biomass carbon (MB-C) and metabolic quotients (qCO$_2$ and qD). Soil samples were taken under natural grass (NG), ...

  1. Monitoring rhizosphere microbial communities of tomato

    OpenAIRE

    Deery, Sarah Jane

    2012-01-01

    Tomato is an economically important crop that can be devastated by many root infecting pathogens. The development of alternative and sustainable crop cultivation techniques and disease control methods is a must for the tomato industry, due to more strict government regulations and concerns over the sustainability of conventional chemical-intensive agriculture (Dixon and Margerison, 2009). In this thesis, the molecular fingerprinting method Terminal-Restriction Fragment Length Polymorphis...

  2. Green manuring effect of pure and mixed barley - hairy vetch winter cover crops on maize and processing tomato N nutrition

    DEFF Research Database (Denmark)

    Tosti, Giacomo; Benincasa, Paolo; Farneselli, Michela

    2012-01-01

    Adopting mixtures between legumes and non legumes can be an efficient tool to merge the advantages of the single species in the fall-sown cover crop practice. Nevertheless there is a lack of information on how the species proportion may affect N accumulation and C/N of the cover crops and how...... tomato (Lycopersicon esculentum Mill.). Cover crop N accumulation and C/N ratio were monitored during the whole growing cycle, and CO2 flux from the soil was measured after their incorporation into the soil. N status of the following cash crops was evaluated by comparing the observed data...... be important to take the characteristics of the following cash crop into account....

  3. Green manuring effect of pure and mixed barley - hairy vetch winter cover crops on maize and processing tomato N nutrition

    DEFF Research Database (Denmark)

    Tosti, Giacomo; Benincasa, Paolo; Farneselli, Michela;

    2012-01-01

    with the appropriate critical N dilution curves. The results highlight the effectiveness of mixtures for the management of the winter cover crop practice. In the two considered years, the species proportion influences the aboveground biomass (ranging from 2.90 to 5.94 Mg ha-1) and N accumulation (ranging from 73......Adopting mixtures between legumes and non legumes can be an efficient tool to merge the advantages of the single species in the fall-sown cover crop practice. Nevertheless there is a lack of information on how the species proportion may affect N accumulation and C/N of the cover crops and how...... tomato (Lycopersicon esculentum Mill.). Cover crop N accumulation and C/N ratio were monitored during the whole growing cycle, and CO2 flux from the soil was measured after their incorporation into the soil. N status of the following cash crops was evaluated by comparing the observed data...

  4. The crop yield gap between organic and conventional agriculture

    NARCIS (Netherlands)

    Ponti, de T.; Rijk, H.C.A.; Ittersum, van M.K.

    2012-01-01

    A key issue in the debate on the contribution of organic agriculture to the future of world agriculture is whether organic agriculture can produce sufficient food to feed the world. Comparisons of organic and conventional yields play a central role in this debate. We therefore compiled and analyzed

  5. The crop yield gap between organic and conventional agriculture

    NARCIS (Netherlands)

    Ponti, de T.; Rijk, H.C.A.; Ittersum, van M.K.

    2012-01-01

    A key issue in the debate on the contribution of organic agriculture to the future of world agriculture is whether organic agriculture can produce sufficient food to feed the world. Comparisons of organic and conventional yields play a central role in this debate. We therefore compiled and analyzed

  6. Altering Conidial Dispersal of Alternaria solani by Modifying Microclimate in Tomato Crop Canopy.

    Science.gov (United States)

    Jambhulkar, Prashant Prakash; Jambhulkar, Nitiprasad; Meghwal, Madanlal; Ameta, Gauri Shankar

    2016-12-01

    Early blight of tomato caused by Alternaria solani, is responsible for severe yield losses in tomato. The conidia survive on soil surface and old dry lower leaves of the plant and spread when suitable climatic conditions are available. Macroclimatic study reveals that highest inoculum concentration of Alternaria spores appeared in May 2012 to 2013 and lowest concentration during January 2012 to 2013. High night temperature positively correlated and significantly (P solani conidial density, tomato leaf damage and microclimatic parameters as compar to tomato alone (T). Tomato-marigold intercropping-plastic mulching treatment (T + M + P) showed 35-39% reduction in disease intensity as compared to tomato alone. When intercropped with tomato, marigold served as barrier to conidial movement and plastic mulching prevented evapotranspiration and reduced the canopy RH that resulted in less germination of A. solani spores. Marigold intercropping and plastic mulching served successfully as physical barrier against conidial dissemination to diminish significantly the tomato foliar damage produced by A. solani.

  7. Total antioxidant capacity, total phenolic content and iron and zinc dialyzability in selected Greek varieties of table olives, tomatoes and legumes from conventional and organic farming.

    Science.gov (United States)

    Drakou, Marina; Birmpa, Angeliki; Koutelidakis, Antonios E; Komaitis, Michael; Panagou, Efstathios Z; Kapsokefalou, Maria

    2015-03-01

    The objective was to compare 10 types of table olives, 11 types of tomatoes and tomato products and 18 types of legumes from conventional or organic farming for selected nutritional properties. All products were tested for their total antioxidant capacity (TAC) (Ferric Reducing Antioxidant Power (FRAP) assay) and total phenolic content (Folin-Ciocalteau method). Tomatoes and legumes were further tested for iron and zinc dialyzability after in vitro digestion. Ascorbic acid content of tomatoes was also measured. The study resulted that the nutritional properties of olives, tomatoes and legumes tested were different among the various cultivars but, in most cases, not between products from organic or conventional farming. Natural black olives, cherry and santorini tomatoes and lentils exhibited superior nutritional properties.

  8. Nutritional properties of cherry tomatoes harvested at different times and grown in an organic cropping Propriedades nutricionais de tomates cereja colhidos em diferentes épocas e cultivado em sistema orgânico

    Directory of Open Access Journals (Sweden)

    Lucinéia de Pinho

    2011-06-01

    Full Text Available The physicochemical characteristics of the cherry tomato cultivated in organic and conventional production systems and harvested at either 30 or 45 days of cropping were evaluated using a randomized, 2x2 factorial design (2 cropping systems x 2 harvesting times with five repetitions. The parameters analyzed were color, centesimal composition, total energetic value, carotenoids and bioactive amine content. Tomatoes harvested at 30 days had higher total soluble solid (TSS content when grown conventionally, but when harvested at 45 days, both conventional and organic tomatoes had similar TSS values, probably due to increased N availability in the soil. Organic cherry tomatoes had higher contents of β-carotene, lycopene and bioactive amine. On the other hand, tomatoes from conventional cropping were more alkaline and brighter. In conclusion, organic tomatoes are more nutritious than conventional varieties, and if allowed to ripen for up to 45 days, contain higher levels of TSS, carotenoids and total bioactive amines.As características físico-químicas de tomates cereja cutivados em sistema orgânico e convencional e colhidos em 30 ou 45 dias de cultivo foram avaliadas em um delineamento casualizado, fatorial 2x2 (2 sistemas de cultivo x 2 épocas de colheita com cinco repetições. Foram analisados os parâmetros cor, composição centesimal, valor energético total e conteúdo de carotenóides e de aminas bioativas. Tomates colhidos em 30 dias tiveram maior conteúdo de sólidos solúveis totais (SST, possivelmente devido ao aumento de disponibilidade de N no solo. Os tomates orgânicos tiveram maior conteúdo de β-caroteno, licopeno e aminas bioativas. Por outro lado, os tomates convencionais foram menos ácidos e tiveram maior brilho. Conclui-se que tomates orgânicos são mais nutritivos que os convencionais, e se amadurecerem até 45 dias concentram maior teor de SST, carotenóides e aminas bioativas totais.

  9. Pepino mosaic virus and Tomato torrado virus: two emerging viruses affecting tomato crops in the Mediterranean basin.

    Science.gov (United States)

    Gómez, Pedro; Sempere, Raqueln; Aranda, Miguel A

    2012-01-01

    The molecular biology, epidemiology, and evolutionary dynamics of Pepino mosaic virus (PepMV) are much better understood than those of Tomato torrado virus (ToTV). The earliest descriptions of PepMV suggest a recent jump from nontomato species (e.g., pepino; Solanum muricatum) to tomato (Solanum lycopersicum). Its stability in contaminated plant tissues, its transmission through seeds, and the global trade of tomato seeds and fruits may have facilitated the global spread of PepMV. Stability and seed transmission also probably account for the devastating epidemics caused by already-established PepMV strains, although additional contributing factors may include the efficient transmission of PepMV by contact and the often-inconspicuous symptoms in vegetative tomato tissues. The genetic variability of PepMV is likely to have promoted the first phase of emergence (i.e., the species jump) and it continues to play an important role as the virus becomes more pervasive, progressing from regional outbreaks to pandemics. In contrast, the long-term progression of ToTV outbreaks is not yet clear and this may reflect factors such as the limited accumulation of the virus in infected plants, which has been shown to be approximately two orders of magnitude less than PepMV. The efficient dispersion of ToTV may therefore depend on dense populations of its principal vectors, Bemisia tabaci and Trialeurodes vaporariorum, as has been proposed for the necrogenic satellite RNA of Cucumber mosaic virus.

  10. Combined Action of Sex Pheromone and Wasp Apanteles gelechiidivoris in Greenhouse Tomato Crops

    Directory of Open Access Journals (Sweden)

    Jessica Andrea Morales

    2014-03-01

    Full Text Available The tomato budworm, Tuta absoluta, is considered main pest of tomato crops. Control of this pest is performed with hemicals, although, there are other strategies such as biological and ethological control. In Colombia there is not precedent that combines both strategies: ethological control with sexual pheromone and biological control with Apanteles gelechiidivoris, for the control of this pest in tomato crops. In this work four different treatments under greenhouse conditions were evaluated including biological control with A. gelechiidivoris, ethological control with sexual pheromone traps, combined action of both controls and traditional control (Chemicals. The experiments aimed to developing a control strategy to reduce populations of T. absoluta. This was done sampling a plant every to 2 meters. From each plant a sample composed by one leaf by stratum was taken and the variables number of total larvae of third instar and parasited and number of captured adults for trap. The maximum parasitism in the population of susceptible larvae was 86.38 % and for total population of larvae was 68.75 %. The combined action of pheromone traps and A. gelechiidivoris presented a greater efficiency and permanence on the control of larvae of T. absoluta.ACCIÓN COMBINADA DE FEROMONA SEXUAL Y DE AVISPAS Apanteles gelechiidivoris PARA EL CONTROL DE Tuta absoluta EN CULTIVOS DE TOMATE BAJO INVERNADEROEl cogollero del tomate, Tuta absoluta, es considerado plaga principal del cultivo de tomate. Su control se realiza con químicos, aunque existen otros tipos de control como el biológico y etológico. En Colombia no existe ningún precedente que involucre la acción combinada de estrategias de control etológico incluyendo feromona sexual y biológico con Apanteles gelechiidivoris para el control de la plaga en cultivos de tomate. En este trabajo, se evaluaron cuatro tratamientos bajo condiciones de invernadero que incluyen control biológico con A

  11. Ground beetle (Coleoptera: Carabidae) assemblages in conventional and diversified crop rotation systems.

    Science.gov (United States)

    O'Rourke, Megan E; Liebman, Matt; Rice, Marlin E

    2008-02-01

    Ground beetles (Coleoptera: Carabidae) are important in agro-ecosystems as generalist predators of invertebrate pests and weed seeds and as prey for larger animals. However, it is not well understood how cropping systems affect ground beetles. Over a 2-yr period, carabids were monitored two times per month using pitfall traps in a conventional chemical input, 2-yr, corn/soybean rotation system and a low input, 4-yr, corn/soybean/triticale-alfalfa/alfalfa rotation system. Carabid assemblages were largely dominated by a few species across all cropping treatments with Poecilus chalcites Say comprising >70% of pitfall catches in both years of study. Overall carabid activity density and species richness were higher in the low input, 4-yr rotation compared with the conventionally managed, 2-yr rotation. There were greater differences in the temporal activity density and species richness of carabids among crops than within corn and soybean treatments managed with different agrichemical inputs and soil disturbance regimes. Detrended correspondence analysis showed strong yearly variation in carabid assemblages in all cropping treatments. The increase in carabid activity density and species richness observed in the 4-yr crop rotation highlights the potential benefits of diverse crop habitats for carabids and the possibility for managing natural enemies by manipulating crop rotations.

  12. Meteorological fluctuations define long-term crop yield patterns in conventional and organic production systems.

    Science.gov (United States)

    Teasdale, John R; Cavigelli, Michel A

    2017-04-06

    Variability in meteorological patterns presents significant challenges to crop production consistency and yield stability. Meteorological influences on corn and soybean grain yields were analyzed over an 18-year period at a long-term experiment in Beltsville, Maryland, U.S.A., comparing conventional and organic management systems. Precipitation and temperature variables explained much of the yield variability, with precipitation and heat stress during the late vegetative and early reproductive phases of crop growth accounting for the majority of yield variability in all crops and management systems. Crop yields under conventional and organic management followed similar periodic patterns, but yields were 31% and 20% lower in organic than conventional corn and soybean, respectively. The efficiency of grain yield per unit precipitation was higher under conventional than organic management, highlighting the importance of crop management for optimizing production in response to meteorological variability. Periodic yield and precipitation patterns did not consistently align with global meteorological cycles such as the El Niño Southern Oscillation.

  13. Organic weed conrol and cover crop residue integration impacts on weed control, quality, and yield and economics in conservation tillage tomato - A case study

    Science.gov (United States)

    The increased use of conservation tillage in vegetable production requires more information be developed on the role of cover crops in weed control, tomato quality and yield. Three conservation-tillage systems utilizing crimson clover, brassica and cereal rye as winter cover crops were compared to ...

  14. OrgTrace – No difference found in bioactive compounds of organic and conventional crops

    DEFF Research Database (Denmark)

    Knuthsen, Pia; Søltoft, Malene; Laursen, Kristian Holst;

    The objective of the present study was to compare the content of selected bioactive compounds in organically and conventionally grown crops, and to evaluate if the ability of the crops to synthesize selected secondary metabolites was systematically affected by growth systems across different growth...... contents of bioactive compounds than the conventionally grown. This indicates that giving preference to organic products because they contain more bioactive components is doubtfull. However, there are many other reasons for the consumer to choose organic food products, including: no pesticide residues...

  15. Algal derivatives may protect crops from residual soil salinity: a case study on a tomato-wheat rotation

    Science.gov (United States)

    Di Stasio, Emilio; Raimondi, Giampaolo; Van Oosten, Michael; Maggio, Albino

    2017-04-01

    In coastal areas, summer crops are frequently irrigated with saline water. As a consequence, salts may accumulate in the root zone with detrimental effects on the following winter crops if the rainfall is insufficient to leach them. Two field experiments were performed in 2015-2016 on a field used for tomato (summer) wheat (winter) rotation cropping. The spring-summer experiment was carried in order to evaluate the effect of two algal derivatives (Ascophyllum nodosum), Rygex and Super Fifty, on a tomato crop exposed to increasing salinity and reduced nutrient availability. In the autumn-winter experiment we investigated the effect of residual salts from the previous summer irrigations on plant growth and yield of wheat treated with the same two algal extracts. The salt treatment for the irrigated summer crop was 80 mM NaCl plus a non-salinized control. The nutrient regimes were 100% and 50% of the tomato nutritional requirements. With both the seaweeds applications the salt stressed plants were demonstrated improved Relative Water Content and water potential. Nevertheless the total fresh biomass and the fruit fresh weight were enhanced only in the non salinized controls. Application of algal derivatives increased the total fresh weight over controls in the non salinized plants. The seaweed treatments enhanced the fruit fresh weight with an increase of 30% and 46% for Rygex and Super Fifty, respectively. Preliminary analysis of the ion profile in roots, shoots and leaves, indicates that the seaweed extracts may enhance the assimilation of ions in fruits affecting their nutritional value. The residual salinity of the summer experiment reduced the wheat biomass production. However, the seaweed extracts treatments improved growth under salinity. In the salt stressed plants the Super Fifty application increased shoots and ears by 34% and 23% respectively, compared to the non treated plants. Plant height was increased by application of seaweeds extracts for both the

  16. Pesticide dissipation curves in peach, pear and tomato crops in Uruguay.

    Science.gov (United States)

    Galietta, Giovanni; Egana, Eduardo; Gemelli, Fernando; Maeso, Diego; Casco, Noelia; Conde, Paula; Nunez, Saturnino

    2011-01-01

    Dissipation curves of azoxystrobin and of the neonicotinoids acetamiprid and thiacloprid in peach; azinphos-methyl and carbaryl in pear and azoxystrobin, chlorfenapyr and chlorpyrifos in high-tunnel tomato crops were studied in the Southern region of Uruguay. An analytical methodology based on solid phase extraction (SPE) and detection by High Performance Liquid Chromatography with Diode Array Detector (HPLC/DAD) was used for acetamiprid and thiacloprid. Coupled SPE and detection by Gas Chromatography with Mass Selective Detector (GC/MSD) was used for the detection of azinphos-methyl, azoxystrobin, carbaryl, chlorfenapyr and chlorpyrifos residues. Curves were modeled mathematically with Solver program of Microsoft Excel. The best fit for acetamiprid and thiacloprid in peach was achieved with the exponential model (r(2)=0.961 and 0.944, respectively). In the case of peach fruits there is not a Maximum Residue Limit (MRL) for acetamiprid in the Codex Alimentarius, while 0.5 mg/kg is the value rated for thiacloprid. The MRLs accepted by the European Union (EU) are 0.1 mg/kg for acetamiprid and 0.3 mg/kg for thiacloprid. According to the curves determined in these experiments, thiacloprid residues 10 to 12 days after application (daa) were below the MRLs established by both sources. In the case of acetamiprid, 25 daa would be required, according to the exponential mathematical model, to get residues levels below the MRL values established by the EU. For azinphos methyl in pear, the residues detected were mathematically fitted to an exponential model (r(2)=0.999). According to it, residue levels under the MRL established by the EU (0.05 mg/kg) are gotten in our conditions in 20 daa. In plastic tunnel tomato chlorfenapyr residues were not detected from 16 daa, having the dissipation curve an exponential trend. In the same condition, there was not a decay of the azoxystrobin concentration during a 24-day trial, being it around 0.40 ± 0.05 mg/kg.

  17. Regional modelling of nitrate leaching from Swiss organic and conventional cropping systems under climate change

    Science.gov (United States)

    Calitri, Francesca; Necpalova, Magdalena; Lee, Juhwan; Zaccone, Claudio; Spiess, Ernst; Herrera, Juan; Six, Johan

    2016-04-01

    Organic cropping systems have been promoted as a sustainable alternative to minimize the environmental impacts of conventional practices. Relatively little is known about the potential to reduce NO3-N leaching through the large-scale adoption of organic practices. Moreover, the potential to mitigate NO3-N leaching and thus the N pollution under future climate change through organic farming remain unknown and highly uncertain. Here, we compared regional NO3-N leaching from organic and conventional cropping systems in Switzerland using a terrestrial biogeochemical process-based model DayCent. The objectives of this study are 1) to calibrate and evaluate the model for NO3-N leaching measured under various management practices from three experiments at two sites in Switzerland; 2) to estimate regional NO3-N leaching patterns and their spatial uncertainty in conventional and organic cropping systems (with and without cover crops) for future climate change scenario A1B; 3) to explore the sensitivity of NO3-N leaching to changes in soil and climate variables; and 4) to assess the nitrogen use efficiency for conventional and organic cropping systems with and without cover crops under climate change. The data for model calibration/evaluation were derived from field experiments conducted in Liebefeld (canton Bern) and Eschikon (canton Zürich). These experiments evaluated effects of various cover crops and N fertilizer inputs on NO3-N leaching. The preliminary results suggest that the model was able to explain 50 to 83% of the inter-annual variability in the measured soil drainage (RMSE from 12.32 to 16.89 cm y-1). The annual NO3-N leaching was also simulated satisfactory (RMSE = 3.94 to 6.38 g N m-2 y-1), although the model had difficulty to reproduce the inter-annual variability in the NO3-N leaching losses correctly (R2 = 0.11 to 0.35). Future climate datasets (2010-2099) from the 10 regional climate models (RCM) were used in the simulations. Regional NO3-N leaching

  18. Microwave flow and conventional heating effects on the physicochemical properties, bioactive compounds and enzymatic activity of tomato puree.

    Science.gov (United States)

    Arjmandi, Mitra; Otón, Mariano; Artés, Francisco; Artés-Hernández, Francisco; Gómez, Perla A; Aguayo, Encarna

    2017-02-01

    Thermal processing causes a number of undesirable changes in physicochemical and bioactive properties of tomato products. Microwave (MW) technology is an emergent thermal industrial process that offers a rapid and uniform heating, high energy efficiency and high overall quality of the final product. The main quality changes of tomato puree after pasteurization at 96 ± 2 °C for 35 s, provided by a semi-industrial continuous microwave oven (MWP) under different doses (low power/long time to high power/short time) or by conventional method (CP) were studied. All heat treatments reduced colour quality, total antioxidant capacity and vitamin C, with a greater reduction in CP than in MWP. On the other hand, use of an MWP, in particular high power/short time (1900 W/180 s, 2700 W/160 s and 3150 W/150 s) enhanced the viscosity and lycopene extraction and decreased the enzyme residual activity better than with CP samples. For tomato puree, polygalacturonase was the more thermo-resistant enzyme, and could be used as an indicator of pasteurization efficiency. MWP was an excellent pasteurization technique that provided tomato puree with improved nutritional quality, reducing process times compared to the standard pasteurization process. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  19. Comparison of lycopene, β-carotene and phenolic contents of tomato using conventional and ecological horticultural practices, and arbuscular mycorrhizal fungi (AMF

    Directory of Open Access Journals (Sweden)

    Ulrichs Christian

    2008-04-01

    Full Text Available

    Tomato fruits are rich in anti-oxidant compounds that have been recognized as benef icial for human health. Horticultural practices can influence the concentration of these secondary metabolites. Arbuscular mycorrhizal fungi (AMF can increase nutrient and water absorption of plants. The experiment, performed under glasshouse, examined whether organically grown ‘Vitella F1’ tomatoes differed in their fruit content of lycopene, β- carotene and total phenols from that found in conventionally grown tomatoes. Treatments were the cultivation methods: conventional, organic, conventional+AMF and organic+AMF. When comparing the cultivation method, no significant differences for the analyzed nutritional parameters were found; only tomatoes grown organically had slightly lower total phenolic contents. In both cultivation methods, tomato plants inoculated with AMF (Glomus sp. built higher lycopene content in fruits than those without inoculation. Organic grown tomatoes increased β-carotene and total phenolic contents in fruits as a result of the AMF treatment. AMF applications increased root fresh weight but not shoot fresh weight. The improved growth and nutrient acquisition in tomato demonstrated the potential of AMF colonization for increased antioxidant compounds in fruits. White fly (Trialeurodes vaporariorum was controlled successfully with application of diatomaceous earth Fossil Shield® 90 in organic treatments as well as with Applaud® in the conventional cultivation methods.

  20. Cobertura vegetal, vermicompost y actividad microbiana del suelo en la producción de tomate Soil cover crop, vermicompost and soil microbial activity in the tomato production

    Directory of Open Access Journals (Sweden)

    Manuel Villarreal-Romero

    Full Text Available Se estudió en el cultivo de tomate, el efecto combinado de fertilización química de N, P y K con aplicación de vermicompost, cobertura vegetal del suelo con Mucuna pruriens y labranza mínima; en contraste, al sistema de labranza convencional con fertilización química y acolchado plástico del suelo; para la nutrición de las plantas, medición de algunos parámetros de calidad del fruto y la actividad microbiana del suelo de 2007 a 2008. Se establecieron cinco tratamientos para la siembra del tomate, en los dos tipos de manejo del cultivo. Los resultados mostraron rendimientos similares y calidad poscosecha (firmeza y pérdida de peso de fruto y absorción de N, P, K, Ca y Mg por las plantas de tomate, entre la labranza convencional del cultivo y el de uso de cobertura vegetal del suelo, labranza mínima y fertilización con vermicompost más 250 N-55 P-100 K. Los frutos procedentes de tratamiento T2 presentaron un comportamiento adecuado en firmeza y pérdida de peso en el estudio poscosecha y este resultado fue estadísticamente igual a los frutos del T5 durante el estudio. Las plantas de M. pruriens acumularon en su biomasa nitrógeno fijado de la atmósfera y residual del suelo en cantidad importante que estuvo disponible para el cultivo de tomate. La colonización micorrízica en las plantas de tomate y la liberación de CO2 del suelo, fueron más altas con la cobertura vegetal y vermicompost que el sistema de manejo convencional del cultivo.The combined effect of N, P and K chemical fertilizers with vermicompost was studied in tomato planting, vegetation cover of the soil with Mucuna pruriens and minimal farming; in contrast to the conventional farming system with chemical fertilizers and plastic soil padding, for plant nutrition, measurement of some fruit quality parameters and soil microbial activity from 2007 to 2008. The tomato underwent five treatments in two types of crop management. Results showed similar yields and

  1. Factors that affect proliferation of Salmonella in tomatoes post-harvest: the roles of seasonal effects, irrigation regime, crop and pathogen genotype.

    Science.gov (United States)

    Marvasi, Massimiliano; Hochmuth, George J; Giurcanu, Mihai C; George, Andrée S; Noel, Jason T; Bartz, Jerry; Teplitski, Max

    2013-01-01

    Fresh fruits and vegetables become increasingly recognized as vehicles of human salmonellosis. Physiological, ecological, and environmental factors are all thought to contribute to the ability of Salmonella to colonize fruits and vegetables pre- and post-harvest. The goal of this study was to test how irrigation levels, fruit water congestion, crop and pathogen genotypes affect the ability of Salmonella to multiply in tomatoes post-harvest. Fruits from three tomato varieties, grown over three production seasons in two Florida locations, were infected with seven strains of Salmonella and their ability to multiply post-harvest in field-grown tomatoes was tested. The field experiments were set up as a two-factor factorial split plot experiment, with the whole-plot treatments arranged in a randomized complete-block design. The irrigation treatment (at three levels) was the whole-plot factor, and the split-plot factor was tomato variety, with three levels. The significance of the main, two-way, and three-way interaction effects was tested using the (type III) F-tests for fixed effects. Mean separation for each significant fixed effect in the model was performed using Tukey's multiple comparison testing procedure. The irrigation regime per se did not affect susceptibility of the crop to post-harvest proliferation of Salmonella. However, Salmonella grew significantly better in water-congested tissues of green tomatoes. Tomato maturity and genotype, Salmonella genotype, and inter-seasonal differences were the strongest factors affecting proliferation. Red ripe tomatoes were significantly and consistently more conducive to proliferation of Salmonella. Tomatoes harvested in the driest, sunniest season were the most conducive to post-harvest proliferation of the pathogen. Statistically significant interactions between production conditions affected post-harvest susceptibility of the crop to the pathogen. UV irradiation of tomatoes post-harvest promoted Salmonella growth.

  2. Factors that affect proliferation of Salmonella in tomatoes post-harvest: the roles of seasonal effects, irrigation regime, crop and pathogen genotype.

    Directory of Open Access Journals (Sweden)

    Massimiliano Marvasi

    Full Text Available MAIN OBJECTIVES: Fresh fruits and vegetables become increasingly recognized as vehicles of human salmonellosis. Physiological, ecological, and environmental factors are all thought to contribute to the ability of Salmonella to colonize fruits and vegetables pre- and post-harvest. The goal of this study was to test how irrigation levels, fruit water congestion, crop and pathogen genotypes affect the ability of Salmonella to multiply in tomatoes post-harvest. EXPERIMENTAL DESIGN: Fruits from three tomato varieties, grown over three production seasons in two Florida locations, were infected with seven strains of Salmonella and their ability to multiply post-harvest in field-grown tomatoes was tested. The field experiments were set up as a two-factor factorial split plot experiment, with the whole-plot treatments arranged in a randomized complete-block design. The irrigation treatment (at three levels was the whole-plot factor, and the split-plot factor was tomato variety, with three levels. The significance of the main, two-way, and three-way interaction effects was tested using the (type III F-tests for fixed effects. Mean separation for each significant fixed effect in the model was performed using Tukey's multiple comparison testing procedure. MOST IMPORTANT DISCOVERIES AND SIGNIFICANCE: The irrigation regime per se did not affect susceptibility of the crop to post-harvest proliferation of Salmonella. However, Salmonella grew significantly better in water-congested tissues of green tomatoes. Tomato maturity and genotype, Salmonella genotype, and inter-seasonal differences were the strongest factors affecting proliferation. Red ripe tomatoes were significantly and consistently more conducive to proliferation of Salmonella. Tomatoes harvested in the driest, sunniest season were the most conducive to post-harvest proliferation of the pathogen. Statistically significant interactions between production conditions affected post-harvest susceptibility

  3. Synergistic Effects of Agronet Covers and Companion Cropping on Reducing Whitefly Infestation and Improving Yield of Open Field-Grown Tomatoes

    Directory of Open Access Journals (Sweden)

    Stella Mutisya

    2016-09-01

    Full Text Available Tomatoes (Lycopersicon esculentum Mill are one of the biggest vegetable crops in the world, supplying a wide range of vitamins, minerals and fibre in human diets. In the tropics, tomatoes are predominantly grown under sub-optimal conditions by subsistence farmers, with exposure to biotic and abiotic stresses in the open field. Whitefly (Bemisia tabaci Gennadius is one of the major pests of the tomato, potentially causing up to 100% yield loss. To control whitefly, most growers indiscriminately use synthetic insecticides which negatively impact the environment, humans, and other natural pest management systems, while also increasing cost of production. This study sought to investigate the effectiveness of agronet covers and companion planting with aromatic basil (Ocimum basilicum L. as an alternative management strategy for whitefly in tomatoes and to evaluate the use of these treatments ontomato growth and yield. Two trials were conducted at the Horticulture Research and Training Field, Egerton University, Njoro, Kenya. Treatments comprised a combination of two factors, (1 growing environment (agronet and no agronet and (2 companion planting with a row of basil surrounding tomato plants, a row of basil in between adjacent rows of tomato, no companion planting. Agronet covers and companion cropping with a row of basil planted between adjacent tomato rows significantly lowered B. tabaci infestation in tomatoes by 68.7%. Better tomato yields were also recorded in treatments where the two treatments were used in combination. Higher yield (13.75 t/ha was obtained from tomatoes grown under agronet cover with a basil row planted in between adjacent rows of the tomato crop compared to 5.9 t/ha in the control. Non-marketable yield was also lowered to5.9 t/ha compared to 9.8 t/ha in the control following the use of the two treatments in combination. The results of this study demonstrate the potential viability of using companion cropping and agronet

  4. The Impact of Broccoli II & Tomato II on European patents in conventional breeding, GMO’s and Synthetic Biology:

    DEFF Research Database (Denmark)

    Minssen, Timo; Nordberg, Ana

    2015-01-01

    . The EBA has also clarified that this applies irrespective of if such claims are formulated in a product-by-process format or as a per se product . Moreover, the combined effect of Broccoli & Tomato I & II opens new opportunities for patenting GMOs - provided that all other patent criteria are also met...... if confronted with similar issues in the context of national implementations of the Biotech Directive, which have taken a very different view than the EBA. Moreover, the fierce European opposition against genetically modified organisms (GMOs) and Synthetic Biology remains a major challenge to the industry...... and Nordberg, A., The Impact of Broccoli II & Tomatoes II on European Patents in Conventional Breeding, GMO's and Synthetic Biology: The Grand Finale of a Juicy Patents Tale? (May 19, 2015). Univ. of Copenhagen Dept. of Economics Discussion. Available at SSRN: http://ssrn.com/abstract=2607865 or http...

  5. Modelling nitrous oxide emissions from organic and conventional cereal-based cropping systems under different management, soil and climate factors

    DEFF Research Database (Denmark)

    Doltra, J; Olesen, Jørgen E; Báez, D;

    2015-01-01

    -based cropping systems. Forage maize was grown in a conventional dairy system at Mabegondo (NW Spain) and wheat and barley in organic and conventional crop rotations at Foulum (NW Denmark). These two European sites represent agricultural areas with high and low to moderate emission levels, respectively. Field...

  6. The Impact of Broccoli II & Tomato II on European patents in conventional breeding, GMO’s and Synthetic Biology:

    DEFF Research Database (Denmark)

    Minssen, Timo; Nordberg, Ana

    2015-01-01

    (GMOs) and Synthetic Biology remains a major obstacle to the industry, research and innovation in an increasingly significant area of science. This paper has also been published as a working paper on SSRN, see: Minssen, Timo and Nordberg, A., The Impact of Broccoli II & Tomatoes II on European Patents...... in Conventional Breeding, GMO's and Synthetic Biology: The Grand Finale of a Juicy Patents Tale? (May 19, 2015). Univ. of Copenhagen Dept. of Economics Discussion. Available at SSRN: http://ssrn.com/abstract=2607865 or http://dx.doi.org/10.2139/ssrn.2607865...

  7. Morphostructural characterization of soil conventionally tilled with mechanized and animal traction with and without cover crop

    Directory of Open Access Journals (Sweden)

    Ricardo Ralisch

    2010-12-01

    Full Text Available The structural stability and restructuring ability of a soil are related to the methods of crop management and soil preparation. A recommended strategy to reduce the effects of soil preparation is to use crop rotation and cover crops that help conserve and restore the soil structure. The aim of this study was to evaluate and quantify the homogeneous morphological units in soil under conventional mechanized tillage and animal traction, as well as to assess the effect on the soil structure of intercropping with jack bean (Canavalia ensiformis L.. Profiles were analyzed in April of 2006, in five counties in the Southern-Central region of Paraná State (Brazil, on family farms producing maize (Zea mays L., sometimes intercropped with jack bean. The current structures in the crop profile were analyzed using Geographic Information Systems (GIS and subsequently principal component analysis (PCA to generate statistics. Morphostructural soil analysis showed a predominance of compact units in areas of high-intensity cultivation under mechanized traction. The cover crop did not improve the structure of the soil with low porosity and compact units that hamper the root system growth. In areas exposed to animal traction, a predominance of cracked units was observed, where roots grew around the clods and along the gaps between them.

  8. Co-existence of GM, conventional and organic crops in developing countries: Main debates and concerns.

    Science.gov (United States)

    Azadi, Hossein; Taube, Friedhelm; Taheri, Fatemeh

    2017-06-05

    The co-existence approach of GM crops with conventional agriculture and organic farming as a feasible agricultural farming system has recently been placed in the center of hot debates at the EU-level and become a source of anxiety in developing countries. The main promises of this approach is to ensure "food security" and "food safety" on the one hand, and to avoid the adventitious presence of GM crops in conventional and organic farming on the other, as well as to present concerns in many debates on implementing the approach in developing countries. Here, we discuss the main debates on ("what," "why," "who," "where," "which," and "how") applying this approach in developing countries and review the main considerations and tradeoffs in this regard. The paper concludes that a peaceful co-existence between GM, conventional, and organic farming is not easy but is still possible. The goal should be to implement rules that are well-established proportionately, efficiently and cost-effectively, using crop-case, farming system-based and should be biodiversity-focused ending up with "codes of good agricultural practice" for co-existence.

  9. Crop yield, root growth, and nutrient dynamics in a conventional and three organic cropping systems with different levels of external inputs and N re-cycling through fertility building crops

    DEFF Research Database (Denmark)

    Thorup-Kristensen, Kristian; Dresbøll, Dorte Bodin; Kristensen, Hanne Lakkenborg

    2012-01-01

    systems based on fertility building crops (green manures and catch crops). In short, the main distinctions were not observed between organic and conventional systems (i.e. C vs. O1, O2 and O3), but between systems based mainly on nutrient import vs. systems based mainly on fertility building crops (C...

  10. Evapotranspiration Modeling by Linear, Nonlinear Regression and Artificial Neural Network in Greenhouse (Case study Reference Crop, Cucumber and Tomato

    Directory of Open Access Journals (Sweden)

    vahid Rezaverdinejad

    2017-01-01

    Full Text Available Introduction: Greenhouse cultivation is a steadily developing agricultural sector throughout the world. In addition, it is known that water is a major issue almost all part of the world especially for countries which have insufficient water source. With this great expansion of greenhouse cultivation, the need of appropriate irrigation management has a great importance. Accurate determination of irrigation scheduling (irrigation timing and frequency is one of the main factors in achieving high yields and avoiding loss of quality in greenhouse tomato and cucumber. To do this, it is fundamental to know the crop water requirements or real evapotranspiration. Accurate estimation on crop water requirement is needed to avoid the excess or deficit water application, with consequent impacts on nutrient availability for plants. This can be done by using appropriate method to determine the crop evapotranspiration (ETc. In greenhouse cultivation, crop transpiration is the most important energy dissipation mechanisms that influence ETc rate. There are a large number of literatures on methods to estimate ETc in greenhouses. ETc can be measured or estimated by direct or indirect methods. The most common direct method estimates ETc from measurements with weighing lysimeters. Thisalsoincludes the evaporation measuring equipment, class A pan, Piche atmometer and modified atmometer. Indirect method includes the measurement of net radiation, temperature, relative humidity, and air vapour pressure deficit. A large number of models have been developed from these measurements to estimate ETc. Due to the fast development of under greenhouse cultivation all around the world, the needs of information on how it affects ETc in greenhouses has to be known and summarized. The existing models for ETc calculation have to be studied to know whether it is reliable for greenhouse climate (hereafter, microclimate or not. Regression and artificial neural network models are two

  11. Organic fertilizer and its effects on the growth and development of tomato crop (Solanum lycopersicum L.

    Directory of Open Access Journals (Sweden)

    Ricardo Augusto Luna Murillo

    2015-04-01

    Full Text Available Organic fertilizers are one of the alternatives in the group of products used in sustainable agri-culture, mainly what is obtained from organic sources of recyclable nature as compost and vermicompost. The aim of this study was to determine the effect of organic fertilizers on the growth and development of tomato plants. The treatments were vermicompost, water hyacinth, and the combination 50 % vermicompost and 50 % water hyacinth control, using a completely randomized design with five replicates. The work was conducted at the Experimental Center La Playita, belonging to the Technical University of Cotopaxi La Maná extension and plant height, number of fruits, fruit diameter, fruit weight was measured. The results showed that the use of organic fertilizers in tomato plant height stimulated with 114.64 cm, number of fruits with 4.08 fruits, fruit diameter with 7.96 mm and weight of 226.50 g fruits, tomato plants

  12. Pesticide residue concentration in soil following conventional and Low-Input Crop Management in a Mediterranean agro-ecosystem, in Central Greece

    Energy Technology Data Exchange (ETDEWEB)

    Karasali, Helen, E-mail: e.karassali@bpi.gr [Laboratory of Chemical Control of Pesticides, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 8 St. Delta Street, Kifissia, 14561 Athens (Greece); Marousopoulou, Anna [Laboratory of Chemical Control of Pesticides, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 8 St. Delta Street, Kifissia, 14561 Athens (Greece); Machera, Kyriaki, E-mail: k.machera@bpi.gr [Laboratory of Pesticides Toxicology, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 8 St. Delta Street, Kifissia, 14561 Athens (Greece)

    2016-01-15

    The present study was focused on the comparative evaluation of pesticide residues, determined in soil samples from Kopaida region, Greece before and after the implementation of Low-Input Crop Management (LCM) protocols. LCM has been suggested as an environmental friendly plant protection approach to be applied on crops growing in vulnerable to pollution ecosystems, with special focus on the site specific problems. In the case of the specific pilot area, the vulnerability was mainly related to the pollution of water bodies from agrochemicals attributed to diffuse pollution primarily from herbicides and secondarily from insecticides. A total of sixty-six soil samples, were collected and analyzed during a three-year monitoring study and the results of the determined pesticide residues were considered for the impact evaluation of applied plant protection methodology. The LCM was developed and applied in the main crops growing in the pilot area i.e. cotton, maize and industrial tomato. Herbicides active ingredients such as ethalfluralin, trifluralin, pendimethalin, S-metolachlor and fluometuron were detected in most samples at various concentrations. Ethalfluralin, which was the active ingredient present in the majority of the samples ranged from 0.01 μg g{sup −1} to 0.26 μg g{sup −1} soil dry weight. However, the amount of herbicides measured after the implementation of LCM for two cropping periods, was reduced by more than 75% in all cases. The method of analysis was based on the simultaneous extraction of the target compounds by mechanical shaking, followed by liquid chromatography mass spectrometric and gas chromatography electron capture (LC–MS/MS and GC–ECD) analysis. - Highlights: • Effect of Low Input Crop Management (LCM) in a vulnerable to pollution ecosystem. • LCM resulted in herbicide residues reduction in the range of 75 and 100% in all cases. • Conventional practices resulted in increased herbicide residues up to 18%. • Anthropogenic

  13. FISH applications for genomics and plant breeding strategies in tomato and other Solanaceous crops

    NARCIS (Netherlands)

    Szinay, D.; Bai, Y.; Visser, R.G.F.; Jong, de J.H.

    2010-01-01

    This paper describes the use of advanced fluorescence in situ hybridization (FISH) technologies for genomics and breeding of tomato and related Solanum species. The first part deals with the major determinants of FISH technology: (1) spatial resolution, which depends on the diffraction limit of the

  14. Environmental impact assessment of dutch tomato crop production in a Venlo glasshouse

    NARCIS (Netherlands)

    Antón, A.; Torrellas, M.; Montero, J.I.; Ruijs, M.N.A.; Vermeulen, P.C.M.; Stanghellini, C.

    2012-01-01

    This environmental impact assessment of the current situation of Dutch tomato production in a Venlo greenhouse in a temperate climate was developed as part of the EUPHOROS project. The project aims to develop a more sustainable greenhouse system with a reduction of external inputs yet with high prod

  15. Cereal yield and quality as affected by N availability in organic and conventional crop rotations in Denmark

    DEFF Research Database (Denmark)

    Doltra, Jordi; Lægdsmand, Mette; Olesen, Jørgen E

    2011-01-01

    The effects of nitrogen (N) availability related to fertilizer type, catch crop management, and rotation composition on cereal yield and grain N were investigated in four organic and one conventional cropping systems in Denmark using the FASSET model. The four-year rotation studied was: spring......-clover were removed from the plots and an equivalent amount of total-N in pig slurry was applied to the cropping system. The conventional rotation included mineral fertilizer and catch crops (CF + C + F), although only non-legume catch crops were used. Measurements of cereal dry matter (DM) at harvest...... loamy soil. DM yield and grain N content were mainly influenced by the type and amount of fertilizer-N at all three locations. Although a catch crop benefit in terms of yield and grain N was observed in most of the cases, a limited N availability affected the cereal production in the four organic...

  16. The need to breed crop varieties suitable for organic farming, using wheat, tomato and broccoli as examples: A review

    NARCIS (Netherlands)

    Lammerts Van Bueren, E.; Jones, S.S.; Tamm, L.; Murphy, K.M.; Myers, J.R.; Leifert, C.; Messmer, M.M.

    2011-01-01

    It is estimated that more than 95% of organic production is based on crop varieties that were bred for the conventional high-input sector. Recent studies have shown that such varieties lack important traits required under organic and low-input production conditions. This is primarily due to selectio

  17. Simulating changes in cropping practises in conventional and glyphosate-tolerant maize. I. Effects on weeds.

    Science.gov (United States)

    Colbach, Nathalie; Fernier, Alice; Le Corre, Valérie; Messéan, Antoine; Darmency, Henri

    2017-04-01

    Herbicide-tolerant (HT) crops such as those tolerant to glyphosate simplify weed management and make it more efficient, at least at short-term. Overreliance on the same herbicide though leads to the spread of resistant weeds. Here, the objective was to evaluate, with simulations, the impact on the advent of glyphosate resistance in weeds of modifications in agricultural practises resulting from introducing HT maize into cropping systems. First, we included a single-gene herbicide resistance submodel in the existing multispecific FLORSYS model. Then, we (1) simulated current conventional and probable HT cropping systems in two European regions, Aquitaine and Catalonia, (2) compared these systems in terms of glyphosate resistance, (3) identified pertinent cultural practises influencing glyphosate resistance, and (4) investigated correlations between cultural practises and species traits, using RLQ analyses. The simulation study showed that, during the analysed 28 years, (1) glyphosate spraying only results in glyphosate resistance in weeds when combined with other cultural factors favouring weed infestation, particularly no till; (2) pre-sowing glyphosate applications select more for herbicide resistance than post-sowing applications on HT crops; and (3) glyphosate spraying selects more for species traits avoiding exposure to the herbicide (e.g. delayed early growth, small leaf area) or compensating for fitness costs (e.g. high harvest index) than for actual resistance to glyphosate, (4) actual resistance is most frequent in species that do not avoid glyphosate, either via plant size or timing, and/or in less competitive species, (5) in case of efficient weed control measures, actual resistance proliferates best in outcrossing species. An advice table was built, with the quantitative, synthetic ranking of the crop management effects in terms of glyphosate-resistance management, identifying the optimal choices for each management technique.

  18. The usage of mutant genotypes in tomato selection for specific traits at the institute for vegetable crops

    Directory of Open Access Journals (Sweden)

    Zdravković Jasmina

    2012-01-01

    Full Text Available In order to find a superdominant gene for yield, a collection of 350 samples with genes mutations and different expression in different phases of growth and development: fruit size, leaf shape, plant architecture, number of fruits, etc., has been studied (through multi-year research. Current seed production at the Institute for Vegetable Crops in Smederevska Palanka includes 26 hybrids and two in acceptation process, of different purposes and characteristics. The most common mutation genes in tomato selection at the Institute are: sp (self-pruning inbuilt in determinant hybrids (Balkan F1, Marko F1, Rebus F1 and varieties (Narvik SPF, SP-109, Adonis, u (uniform ripening - Lido F1, Atina F1, Danubius F1, rin (ripening inhibitor- Nada F1, Šampion F1, Sef F1, Sidra F1, Rebus F1, j (jointless - Narvik, Adonis, SP-109, c (potato leaf - Mi-13, Enigma F1, Rebus F1, Ph -2 (Phytophtora infestans resistance - Luna F1, Zlatni jubilej F1, Sef F1. Quality of tomato fruits is defined by two groups of genes which determinate the organoleptic traits and nutrition composition. Organoleptic quality includes taste and smell, colour and texture of fruits established in variety differences, nutritive regime of plants, maturity phase and yield. Little is known about gene regulation, responsible for variation of these traits.

  19. Biological nitrogen fixation in three long-term organic and conventional arable crop rotation experiments in Denmark

    DEFF Research Database (Denmark)

    Pandey, Arjun; Li, Fucui; Askegaard, Margrethe

    2017-01-01

    . The experiment included 4-year crop rotations with three treatment factors in a factorial design: (i) rotations, i.e. organic with a year of grass-clover (OGC), organic with a year of grain legumes (OGL), and conventional with a year of grain legumes (CGL), (ii) with (+CC) and without (−CC) cover crops, and (iii...

  20. Tomato Leaf Curl New Delhi Virus: An Emerging Virus Complex Threatening Vegetable and Fiber Crops

    Directory of Open Access Journals (Sweden)

    Enrique Moriones

    2017-09-01

    Full Text Available The tomato leaf curl New Delhi virus (ToLCNDV (genus Begomovirus, family Geminiviridae represents an important constraint to tomato production, as it causes the most predominant and economically important disease affecting tomato in the Indian sub-continent. However, in recent years, ToLCNDV has been fast extending its host range and spreading to new geographical regions, including the Middle East and the western Mediterranean Basin. Extensive research on the genome structure, protein functions, molecular biology, and plant–virus interactions of ToLCNDV has been conducted in the last decade. Special emphasis has been given to gene silencing suppression ability in order to counteract host plant defense responses. The importance of the interaction with DNA alphasatellites and betasatellites in the biology of the virus has been demonstrated. ToLCNDV genetic variability has been analyzed, providing new insights into the taxonomy, host adaptation, and evolution of this virus. Recombination and pseudorecombination have been shown as motors of diversification and adaptive evolution. Important progress has also been made in control strategies to reduce disease damage. This review highlights these various achievements in the context of the previous knowledge of begomoviruses and their interactions with plants.

  1. Tomato Leaf Curl New Delhi Virus: An Emerging Virus Complex Threatening Vegetable and Fiber Crops.

    Science.gov (United States)

    Moriones, Enrique; Praveen, Shelly; Chakraborty, Supriya

    2017-09-21

    The tomato leaf curl New Delhi virus (ToLCNDV) (genus Begomovirus, family Geminiviridae) represents an important constraint to tomato production, as it causes the most predominant and economically important disease affecting tomato in the Indian sub-continent. However, in recent years, ToLCNDV has been fast extending its host range and spreading to new geographical regions, including the Middle East and the western Mediterranean Basin. Extensive research on the genome structure, protein functions, molecular biology, and plant-virus interactions of ToLCNDV has been conducted in the last decade. Special emphasis has been given to gene silencing suppression ability in order to counteract host plant defense responses. The importance of the interaction with DNA alphasatellites and betasatellites in the biology of the virus has been demonstrated. ToLCNDV genetic variability has been analyzed, providing new insights into the taxonomy, host adaptation, and evolution of this virus. Recombination and pseudorecombination have been shown as motors of diversification and adaptive evolution. Important progress has also been made in control strategies to reduce disease damage. This review highlights these various achievements in the context of the previous knowledge of begomoviruses and their interactions with plants.

  2. The genetic basis of fruit morphology in horticultural crops: lessons from tomato and melon.

    Science.gov (United States)

    Monforte, Antonio J; Diaz, Aurora; Caño-Delgado, Ana; van der Knaap, Esther

    2014-08-01

    Fruits represent an important part of the human diet and show extensive variation in size and shape between and within cultivated species. The genetic basis of such variation has been studied most extensively in tomato, where currently six quantitative trait loci (QTLs) involving these traits have been fine-mapped and the genes underlying the QTLs identified. The genes responsible for the cloned QTLs belong to families with a few to many members. FASCIATED is encoded by a member of the YABBY family, CNR/FW2.2 by a member of the Cell Number Regulator family, SlKLUH/FW3.2 by a cytochrome P450 of the 78A class (CYP78A), LOCULE NUMBER by a member of the WOX family including WUSCHEL, OVATE by a member of the Ovate Family Proteins (OFP), and SUN by a member of the IQ domain family. A high portion of the history and current diversity in fruit morphology among tomato cultivars can be explained by modifications at four of these cloned QTLs. In melon, a number of QTLs involved in fruit morphology have been mapped, but the molecular basis for these QTLs is unknown. In the present review, we examine the current knowledge on the molecular basis of fruit morphology in tomato and transfer that information in order to define candidate genes of melon fruit shape and size QTLs. We hypothesize that different members of the gene families identified in tomato may have a role in the regulation of fruit morphology in other species. We anchored the published melon QTL map on the genome sequence and identified the melon family members of the six cloned tomato QTLs in the genome. We investigated the co-localization of melon fruit morphology QTLs and the candidate genes. We found that QTLs for fruit weight co-localized frequently with members of the CNR/FW2.2 and KLUH/FW3.2 families, as well as co-localizations between OFP family members and fruit-shape QTLs, making this family the most suitable to explain fruit shape variation among melon accessions.

  3. Coexistence between conventional, organic and GM crops production: the Portuguese system.

    Science.gov (United States)

    Chiarabolli, Alessandro

    2011-01-01

    The objective of this paper is to analyze the way of Portugal is addressing the issue of the coexistence between conventional, organic and Genetically Modified (GM) crops production. In the EU, no form of agriculture, be it conventional, organic or transgenic, should be excluded. Farmers are free to choose the production type they prefer, without being forced to change patterns already established in the area and without spending more resources. Farmers' choice of growing GM or non-GM crops depends not only on technical aspects related to the productivity gains and agronomic benefits to be gained from adopting this technology, but also on consumers' preferences. Today only few Member States have adopted specific legislation on coexistence. Portugal was one of the first European Country that, in 2005, adopted a coexistence law and it has implemented one of the most complete systems of coexistence regulation. Today Portugal has a well-balanced regime based on free choice for consumers and growers. It has a coexistence system complete regulation and farmers who wish to cultivate GM maize must fulfill with national coexistence legislation that includes the following compulsory rules: participate in specific coexistence training courses, register the cultivation area, inform by written notification about their intent to cultivate GM, apply measures of coexistence, among others.

  4. Crop yield, root growth, and nutrient dynamics in a conventional and three organic cropping systems with different levels of external inputs and N re-cycling through fertility building crops

    DEFF Research Database (Denmark)

    Thorup-Kristensen, Kristian; Dresbøll, Dorte Bodin; Kristensen, Hanne Lakkenborg

    2012-01-01

    to reduce the reliance on import of external resources significantly. We compared a conventional system (C) and an organic system relying on manure import for soil fertility (O1) to two novel systems (O2 and O3) all based on the same crop rotation. The O2 and O3 systems represented new versions...... systems based on fertility building crops (green manures and catch crops). In short, the main distinctions were not observed between organic and conventional systems (i.e. C vs. O1, O2 and O3), but between systems based mainly on nutrient import vs. systems based mainly on fertility building crops (C......One of the core ideas behind organic production is that cropping systems should be less dependent on import of resources, and minimize negative effects on the surrounding environment compared to conventional production. However, even when clearly complying with regulations for organic production...

  5. Potential pollinators of tomato, Lycopersicon esculentum (Solanaceae), in open crops and the effect of a solitary bee in fruit set and quality.

    Science.gov (United States)

    Santos, A O R; Bartelli, B F; Nogueira-Ferreira, F H

    2014-06-01

    We identified native bees that are floral visitors and potential pollinators of tomato in Cerrado areas, described the foraging behavior of these species, and verified the influence of the visitation of a solitary bee on the quantity and quality of fruits. Three areas of tomato crops, located in Minas Gerais, Brazil, were sampled between March and November 2012. We collected 185 bees belonging to 13 species. Exomalopsis (Exomalopsis) analis Spinola, 1853 (Hymenoptera: Apidae) was the most abundant. Ten species performed buzz pollination. Apis mellifera L. 1758 (Hymenoptera: Apidae) and Paratrigona lineata (Lepeletier, 1836) (Hymenoptera: Apidae) could also act as pollinators. The fruit set and number of seeds obtained from the pollination treatment by E. analis were higher than those in the control group. Our results allowed the identification of potential tomato pollinators in Cerrado areas and also contributed information regarding the impact of a single species (E. analis) on fruit set and quality. Although most of the visiting bees show the ability for tomato pollination, there is an absence of adequate management techniques, and its usage is difficult with the aim of increasing the crop production, which is the case for E. analis. Species such as Melipona quinquefasciata, P. lineata, and A. mellifera, which are easy to handle, are not used for pollination services. Finally, it is suggested that a combination of different bee species that are able to pollinate the tomato is necessary to prevent the super-exploitation of only a single species for pollination services and to guarantee the occurrence of potential pollinators in the crop area.

  6. Cowpea production as affected by dry spells in no-tillage and conventional crop systems

    Directory of Open Access Journals (Sweden)

    Rômulo Magno Oliveira de Freitas

    2013-12-01

    Full Text Available The objective of this study was to evaluate the effect of different periods of water shortage in no-tillage and conventional crop systems on cowpea yield components and grain yield in the Mossoró-RN region. For this, an experiment was conducted using two tillage systems (conventional and no-tillage subjected to periods of irrigation suspension (2; 6; 10; 14; 18 end 22 days, started at flowering (34 days after sowing. Plants were harvested 70 days after sowing, and the studied variables were: Pods length (CV, number of grains per pod (NGV, number of pods per plant (NPP, the hundred grains weight (PCG and grain yield (kg ha-1. The no-tillage system is more productive than the conventional under both irrigation and water stress treatments. The water stress length affected grain yield and all yield components studied in a negative way, except for the hundred grains weight. Among the systems studied, the no-tillage provides higher values for the yield components, except the hundred grains weight.

  7. A field-grown transgenic tomato line expressing higher levels of polyamines reveals legume cover crop mulch-specific perturbations in fruit phenotype at the levels of metabolite profiles, gene expression, and agronomic characteristics.

    Science.gov (United States)

    Neelam, Anil; Cassol, Tatiana; Mehta, Roshni A; Abdul-Baki, Aref A; Sobolev, Anatoli P; Goyal, Ravinder K; Abbott, Judith; Segre, Anna L; Handa, Avtar K; Mattoo, Autar K

    2008-01-01

    Genetic modification of crop plants to introduce desirable traits such as nutritional enhancement, disease and pest resistance, and enhanced crop productivity is increasingly seen as a promising technology for sustainable agriculture and boosting food production in the world. Independently, cultural practices that utilize alternative agriculture strategies including organic cultivation subscribe to sustainable agriculture by limiting chemical usage and reduced tillage. How the two together affect fruit metabolism or plant growth in the field or whether they are compatible has not yet been tested. Fruit-specific yeast S-adenosylmethionine decarboxylase (ySAMdc) line 579HO, and a control line 556AZ were grown in leguminous hairy vetch (Vicia villosa Roth) (HV) mulch and conventional black polyethylene (BP) mulch, and their fruit analysed. Significant genotypexmulch-dependent interactions on fruit phenotype were exemplified by differential profiles of 20 fruit metabolites such as amino acids, sugars, and organic acids. Expression patterns of the ySAMdc transgene, and tomato SAMdc, E8, PEPC, and ICDHc genes were compared between the two lines as a function of growth on either BP or HV mulch. HV mulch significantly stimulated the accumulation of asparagine, glutamate, glutamine, choline, and citrate concomitant with a decrease in glucose in the 556AZ fruits during ripening as compared to BP. It enables a metabolic system in tomato somewhat akin to the one in higher polyamine-accumulating transgenic fruit that have higher phytonutrient content. Finally, synergism was found between HV mulch and transgenic tomato in up-regulating N:C indicator genes PEPC and ICDHc in the fruit.

  8. Field performance of alternate wetting and drying furrow irrigation on tomato crop growth, yield, water use efifciency, quality and proiftability

    Institute of Scientific and Technical Information of China (English)

    Khokan Kumer Sarker; M A R Akanda; S K Biswas; D K Roy; A Khatun; M A Goffar

    2016-01-01

    Sustainable irrigation method is now essential for adaptation and adoption in the areas where water resources are limited. Therefore, a ifeld experiment was conducted to test the performance of alternate wetting and drying furrow irrigation (AWDFI) on crop growth, yield, water use efifciency (WUE), fruit quality and proiftability analysis of tomato. The experiment was laid out in randomized complete block design with six treatments replicated thrice during the dry seasons of 2013-2014 and 2014-2015. Irrigation water was applied through three ways of furrow: AWDFI, ifxed wetting and drying furrow irrigation (FWDFI) and traditional (every) furrow irrigation (TFI). Each irrigation method was divided into two levels: irrigation up to 100 and 80% ifeld capacity (FC). Results showed that plant biomass (dry matter) and marketable fruit yield of tomato did not differ signiifcantly between the treatments of AWDFI and TFI, but signiifcant difference was observed in AWDFI and in TFI compared to FWDFI at same irrigation level. AWDFI saved irrigation water by 35 to 38% for the irrigation levels up to 80 and 100% FC, compared to the TFI, respectively. AWDFI improved WUE by around 37 to 40% compared to TFI when irrigated with 100 and 80% FC, respectively. Fruit quality (total soluble solids and pulp) was found greater in AWDFI than in TFI. Net return from AWDFI technique was found nearly similar compared to TFI and more than FWDFI. The beneift cost ratio was viewed higher in AWDFI than in TFI and FWDFI by 2.8, 8.7 and 11, 10.4% when irrigation water was applied up to 100 and 80% FC, respectively. Unit production cost was obtained lower in AWDFI compared to TFI and FWDFI. However, AWDFI is a useful water-saving furrow irrigation technique which may resolve as an alternative choice compared with TFI in the areas where available water and supply methods are limited to irrigation.

  9. Fruit quality and bioactive compounds with antioxidant activity of tomatoes grown on-farm: comparison of organic and conventional management systems.

    Science.gov (United States)

    Juroszek, Peter; Lumpkin, Heidi M; Yang, Ray-Yu; Ledesma, Dolores R; Ma, Chin-Hua

    2009-02-25

    Replicated field trials at three matched farm pairs in southern and central Taiwan were established in October 2004 and 2005 to compare fruit quality and nutritional parameters of tomatoes grown on-farm under organic versus conventional management systems in tropical and subtropical environments. Two processing tomato varieties were evaluated using a randomized complete block design at each of the farms. Aggregation of farms by type (organic vs conventional) across two years resulted in no significant differences between organic and conventional farming systems for all tomato fruit parameters measured, including quality (pH, soluble solids, acidity, and color), content of bioactive compounds with antioxidant activity (beta-carotene, lycopene, ascorbic acid, and total phenolics), and antioxidant activity. This study indicated no consistent effect of the farming system on tomato fruit parameters. Farm management skills combined with site-specific effects contributed to high lycopene levels, and the choice of variety significantly influenced the content of bioactive compounds, particularly ascorbic acid and total phenolics.

  10. Integrating ecosystem services into crop protection and pest management: Case study with the soil fumigant 1,3-dichloropropene and its use in tomato production in Italy.

    Science.gov (United States)

    Deacon, Samantha; Alix, Anne; Knowles, Steve; Wheeler, James; Tescari, Enzo; Alvarez, Lara; Nicolette, Joseph; Rockel, Mark; Burston, Peter; Quadri, Giorgia

    2016-10-01

    Ecosystems provide the conditions for producing food, regulating water, and providing wildlife habitats; these, among others, are known as ecosystem services (ESs). Food production is both economically and culturally important to southern European farmers, particularly in Italy where farmers grow flavorsome tomatoes with passion and pride. Growers rely on pesticides for crop protection, the potential environmental impact of which is often questioned by regulators and other stakeholders. The European regulatory system for the approval of pesticides includes a thorough evaluation of risks to the environment and is designed to be protective of ecosystems. The consideration of ESs in environmental decision making is a growing trend, and the present case study provides an example of how ESs evaluation could be used to enhance agricultural practices and regulatory policy for crop protection. By attacking plant roots, nematodes may affect the growth and yield of fruit and vegetable crops, and the income earned by farmers at harvest time. Available solutions include chemical treatments such as 1,3-dichloropropene (1,3-D), physical treatments (solarization), and biological treatments (biofumigation). In order to characterize the risks and benefits associated with the use of 1,3-D in crop protection, ESs and socioeconomic analyses were applied to its use in the control of nematodes in tomato cultivation in southern Italy. The present study confirmed the benefits of 1,3-D to tomato production in Italy, with significant positive effects on production yields and farm income when compared to limited and transient potential impacts on services such as soil function. It was confirmed that 1,3-D allows farm income to be maintained and secures tomato production in these regions for the future. Integr Environ Assess Manag 2016;12:801-810. © 2016 SETAC. © 2016 SETAC.

  11. Tensiometer-based irrigation management of subirrigated soilless tomato: effects of substrate matric potential control on crop performance

    Directory of Open Access Journals (Sweden)

    Francesco Fabiano eMontesano

    2015-12-01

    the matric potential, while in ‘Diana’ there was no increase. Control of substrate water status offer the possibility to steer crop response by enhancing different crop-performance components, namely yield and fruit quality, in subirrigated tomato. Small-size fruit varieties benefit more from controlled water stress in terms of reduced unmarketable yield loss and fruit quality improvements.

  12. Interferência das plantas daninhas na cultura do tomate para processamento Weed interference in processing tomato crops

    Directory of Open Access Journals (Sweden)

    Adriano Stephan Nascente

    2004-09-01

    Full Text Available Para se alcançar eficiência no manejo cultural, é muito importante determinar o período crítico de interferência (PCI das plantas daninhas no cultivo das hortaliças. Avaliou-se neste trabalho a interferência das plantas daninhas na cultura do tomate para processamento, pelo transplantio de mudas, na Embrapa Hortaliças, em delineamento de blocos ao acaso com 3 repetições. Os tratamentos foram divididos em dois grupos, com períodos iniciais crescentes, denominados sem a interferência (no limpo e com interferência (no mato das plantas daninhas. No primeiro, a cultura de tomate permaneceu livre da interferência das plantas daninhas desde o transplantio das mudas até os seguintes períodos (dias do ciclo de desenvolvimento do tomate: 1-28, 1-35, 1-49, 1-63, 1-77, 1-91 (todo ciclo, colheita. Após estes períodos, as plantas daninhas cresceram livremente até o final do ciclo da cultura. No segundo grupo a cultura permaneceu com a interferência das plantas daninhas desde o preparo final do solo e transplantio das mudas até os mesmos períodos descritos para o primeiro grupo. Após estes períodos as plantas daninhas foram removidas manualmente até a colheita. De um total de 24, as espécies mais freqüentes e/ou de maior acúmulo de matéria seca foram: Bidens pilosa, Brachiaria plantaginea, Nicandra physaloides e Oxalis latifolia. As plantas daninhas causaram reduções na produção de tomate de até 75,5%, sendo que o PCI ocorreu no período do 33º ao 76º dia após a implantação da cultura.Weed control efficiency depends on the information about its interference on the crops. The interference of weed on tomato plants of a processing variety, transplanted directly into the field was evaluated. The field experiment was carried out, in a randomized block design with three replications, at Embrapa Hortaliças in Brasília, Brazil. Plots were laid out with increasing periods of time in the presence or absence of weeds. In the

  13. Working conditions of women farmers growing tomato crop in greenhouses; a case in Sáchica, Boyacá

    Directory of Open Access Journals (Sweden)

    Natalia Marcela Espinosa Becerra

    2013-12-01

    Full Text Available This research sought from the Social Work approach to analyze the influence of the working conditions of greenhouse tomato crops in the municipality of Sáchica (Boyacá in the health and productive roles, reproductive and community of rural women there laboratory, using qualitative research retaking narrative elements, crosscutting ethnography and participative techniques from the intervention of women working in rotation in the greenhouses of this municipality and owners there. It has been shown that these rural agricultural practices in the area are an important source of income for rural women and their families, however, the employment situation is not conceived in balance with the needs of the workers, from the perspective of the risks they face every day to the detriment of their health. In this sense, it aims to provide a look that invite thinking from academic and institutional spheres, despite a change in the concept we have of rural women since its multiple dimensions, which are not only productive but also social, cultural and political.

  14. Differences in soil quality between organic and conventional farming over a maize crop season

    Science.gov (United States)

    Ferreira, Carla; Veiga, Adelcia; Puga, João; Kikuchi, Ryunosuke; Ferreira, António

    2017-04-01

    Land degradation in agricultural areas is a major concern. The large number of mechanical interventions and the amount of inputs used to assure high crop productivity, such as fertilizers and pesticides, have negative impacts on soil quality and threaten crop productivity and environmental sustainability. Organic farming is an alternative agriculture system, based on organic fertilizers, biological pest control and crop rotation, in order to mitigate soil degradation. Maize is the third most important cereal worldwide, with 2008 million tons produced in 2013 (IGN, 2016). In Portugal, 120000 ha of arable land is devoted to maize production, leading to annual yields of about 930000 ton (INE, 2015). This study investigates soil quality differences in maize farms under organic and conventional systems. The study was carried out in Coimbra Agrarian Technical School (ESAC), in central region of Portugal. ESAC campus comprises maize fields managed under conventional farming - Vagem Grande (32 ha), and organic fields - Caldeirão (12 ha), distancing 2.8 km. Vagem Grande has been intensively used for grain maize production for more than 20 years, whereas Caldeirão was converted to organic farming in 2008, and is being used to select regional maize varieties. The region has a Mediterranean climate. The maize fields have Eutric Fluvisols, with gentle slopes (crop season. Each plot comprises 5 plant lines (˜4 m width) with 20 m length. In order to assure the comparison between both farms, the same maize variety was used (Pigarro) in both fields, with the same compass. Soil samples were collected immediately after sowing. In Vagem Grande distinct soil samples were taken: (i) within plant lines, and (ii) between plant lines, since mineral fertilizers were spread over the field before sowing, and addition fertilizer was applied together with seeds, in plant lines. In Caldeirão, since fertilization was not performed due to weather constrains, soil samples were collected

  15. Comparison of Crop Rotation for Verticillium Wilt Management and Effect on Pythium Species in Conventional and Organic Strawberry Production

    OpenAIRE

    Subbarao, Krishna V.

    2009-01-01

    The effects of broccoli and lettuce rotations on population densities of Verticillium dahliae and Pythium spp. in soil and on strawberry (Fragaria × ananassa) growth, yield, and Verticillium wilt were evaluated in conventional and organic production systems in California for 2 years. Under both management systems, strawberry was planted after two successive crops of broccoli or lettuce. The control treatment in the conventional field was strawberry planted in soils fumigated with methyl bromi...

  16. Tensiometer-Based Irrigation Management of Subirrigated Soilless Tomato: Effects of Substrate Matric Potential Control on Crop Performance.

    Science.gov (United States)

    Montesano, Francesco F; Serio, Francesco; Mininni, Carlo; Signore, Angelo; Parente, Angelo; Santamaria, Pietro

    2015-01-01

    Automatic irrigation scheduling based on real-time measurement of soilless substrate water status has been recognized as a promising approach for efficient greenhouse irrigation management. Identification of proper irrigation set points is crucial for optimal crop performance, both in terms of yield and quality, and optimal use of water resources. The objective of the present study was to determine the effects of irrigation management based on matric potential control on growth, plant-water relations, yield, fruit quality traits, and water-use efficiency of subirrigated (through bench system) soilless tomato. Tensiometers were used for automatic irrigation control. Two cultivars, "Kabiria" (cocktail type) and "Diana" (intermediate type), and substrate water potential set-points (-30 and -60 hPa, for "Diana," and -30, -60, and -90 hPa for "Kabiria"), were compared. Compared with -30 hPa, water stress (corresponding to a -60 hPa irrigation set-point) reduced water consumption (14%), leaf area (18%), specific leaf area (19%), total yield (10%), and mean fruit weight (13%), irrespective of the cultivars. At -60 hPa, leaf-water status of plants, irrespective of the cultivars, showed an osmotic adjustment corresponding to a 9% average osmotic potential decrease. Total yield, mean fruit weight, plant water, and osmotic potential decreased linearly when -30, -60, and -90 hPa irrigation set-points were used in "Kabiria." Unmarketable yield in "Diana" increased when water stress was imposed (187 vs. 349 g·plant(-1), respectively, at -30 and -60 hPa), whereas the opposite effect was observed in "Kabiria," where marketable yield loss decreased linearly [by 1.05 g·plant(-1) per unit of substrate water potential (in the tested range from -30 to -90 hPa)]. In the second cluster, total soluble solids of the fruit and dry matter increased irrespective of the cultivars. In the seventh cluster, in "Diana," only a slight increase was observed from -30 vs. -60 hPa (3.3 and 1

  17. The use of biodegradable mulch for tomato and broccoli production: Crop yield and quality, mulch deterioration, and growers' perceptions

    Science.gov (United States)

    Cowan, Jeremy Scott

    Biodegradable mulch may offer the benefits of polyethylene mulch for crop production with the added benefit of biodegradability. Four studies were carried out in Mount Vernon, WA to evaluate biodegradable mulch for tomato (Solanum lycopersicum L.) and broccoli (Brassica oleracea var. italica) production. The first study compared four biodegradable mulch treatments: BioAgri, BioTelo, WeedGuardPlus (cellulose product), and SB-PLA-10/11/12 (experimental, non-woven fabric), to polyethylene mulch and bare ground in high tunnels and in the open field for tomato yield and fruit quality over three growing seasons. Biodegradable plastic films produced yields and fruit quality comparable to polyethylene. Moreover, high tunnels increased total and marketable fruit weight five and eight times, respectively, compared to the open field. The second study quantified relationships among visual assessment parameters and mulch mechanical properties. Visual assessments and mechanical property tests of polyethylene, BioAgri, BioTelo, WeedGuardPlus, and SB-PLA-10/11/12, were made over three growing seasons. Regression analyses found the strongest relationship overall (r2 = 0.41) to be between the percent of initial breaking force in the machine direction and log 10 of percent visual deterioration. However, evaluating mulch products individually and increasing sample frequency are recommended for future research. The third study evaluated three biodegradable mulch products, BioAgri, Crown 1, and SB-PLA-11, after soil-incorporation. The average area of recovered mulch fragments decreased for all mulch products over time. The number of mulch fragments initially increased for all mulch products, with the greatest number of Crown 1 and BioAgri fragments recovered 132 and 299 days after incorporation, respectively. At 397 days after soil-incorporation, the total area of recovered fragments of Crown 1 and BioAgri was 0% and 34% of the theoretical maximum area, respectively. The fourth study

  18. Effect of plant diversification on abundance of South American tomato pinworm and predators in two cropping systems Efeito da diversificação de plantas na abundância da traça-do-tomateiro e predadores em dois sistemas de cultivo

    Directory of Open Access Journals (Sweden)

    Maria A Medeiros

    2009-09-01

    Full Text Available Several studies have demonstrated that the diversification of the agroecosystem may reduce insect pest infestation using the strategy of companion planting. We studied the effect of the presence of coriander and Gallant soldier as companion plants in the tomato crop under organic and conventional management on abundance of the tomato pests and predators. The experiment was carried out under field conditions, at Distrito Federal, Brazil, from July to October, 2004. The experimental design was of randomized blocks with three replicates in a 3 x 2 factorial arrangement [Three crop diversification levels: tomato in monoculture, tomato intercropped with coriander, and tomato intercropped with coriander and Gallant soldier; and two cropping systems: organic and conventional]. Each plot had 80 plants of the tomato and the pest-insects and predaceous arthropods were collected every week in five tomato plants/plot. Tuta absoluta (Meyr. was the commonest pest species in both cropping systems. The tomato intercropped with coriander and Gallant soldier in organic cropping system had lower abundance and higher diversity of pests, and higher abundance and diversity of predaceous arthropods, mainly at 80 days after the tomato transplanting, coinciding with the population peak of T. absoluta (eggs and adults. The more abundant predators were spiders, lady beetles and ants. The spiders were abundant and uniformly distributed in both systems. The lady beetles appeared lately in the organic system, at the flowering season of the coriander. The ants were more abundant in the conventional systems.Vários estudos têm demonstrado que a diversificação do agroecossistema pode reduzir a infestação por insetos-praga pelo uso da estratégia do estabelecimento de plantas companheiras. Neste trabalho estudou-se o efeito da presença do coentro e botão-de-ouro como plantas companheiras na cultura do tomate sob manejo orgânico e convencional, na abundância das pragas

  19. Eleven-year results on soft and durum wheat crops grown in an organic and in a conventional low input cropping system

    Directory of Open Access Journals (Sweden)

    Paolo Benincasa

    2016-05-01

    Full Text Available Eleven-year results on yields and apparent balances of organic matter and nitrogen (N are reported for soft and durum wheat crops grown in the BIOSYST long-term experiment for the comparison between an organic and a conventional low-input system in Central Italy. The N supply to organic wheat consisted of 40 kg N ha–1 as poultry manure plus the supposed residual N left by green manures carried out before the preceding summer vegetable, while the N supply to conventional wheat consisted of 80 kg N ha–1 as mineral fertilisers, split in two applications of 40 kg ha–1 each, at tillering and pre-shooting. In every year, above ground biomass and N accumulation of each wheat species, including weeds, and the partitioning between grain yield and crop residues were determined. Apparent dry matter and N balances were calculated at the end of each crop cycle by taking into account the amounts of dry matter and N supplied to the system as fertilisers, and those removed with grain yield. Soft wheat yielded more than durum wheat. For both species, grain yield and protein content were more variable across years and generally lower in the organic than in the conventional system. In both systems, grain yield of both species resulted negatively correlated with fall-winter rainfall, likely for its effect on soil N availability. Both species caused a lower return of biomass and a higher soil N depletion in the organic than in the conventional system. Our experiment confirmed that winter wheat can help exploit the soil N availability and reduce N leaching in fall winter, especially after summer vegetables, but in stockless or stock-limited organic systems it needs to be included in rotations where soil fertility is restored by fall winter green manures to be carried out before summer crops.

  20. Simulating changes in cropping practices in conventional and glyphosate-resistant maize. II. Weed impacts on crop production and biodiversity.

    Science.gov (United States)

    Colbach, Nathalie; Darmency, Henri; Fernier, Alice; Granger, Sylvie; Le Corre, Valérie; Messéan, Antoine

    2017-05-01

    Overreliance on the same herbicide mode of action leads to the spread of resistant weeds, which cancels the advantages of herbicide-tolerant (HT) crops. Here, the objective was to quantify, with simulations, the impact of glyphosate-resistant (GR) weeds on crop production and weed-related wild biodiversity in HT maize-based cropping systems differing in terms of management practices. We (1) simulated current conventional and probable HT cropping systems in two European regions, Aquitaine and Catalonia, with the weed dynamics model FLORSYS; (2) quantified how much the presence of GR weeds contributed to weed impacts on crop production and biodiversity; (3) determined the effect of cultural practices on the impact of GR weeds and (4) identified which species traits most influence weed-impact indicators. The simulation study showed that during the analysed 28 years, the advent of glyphosate resistance had little effect on plant biodiversity. Glyphosate-susceptible populations and species were replaced by GR ones. Including GR weeds only affected functional biodiversity (food offer for birds, bees and carabids) and weed harmfulness when weed effect was initially low; when weed effect was initially high, including GR weeds had little effect. The GR effect also depended on cultural practices, e.g. GR weeds were most detrimental for species equitability when maize was sown late. Species traits most harmful for crop production and most beneficial for biodiversity were identified, using RLQ analyses. None of the species presenting these traits belonged to a family for which glyphosate resistance was reported. An advice table was built; the effects of cultural practices on crop production and biodiversity were synthesized, explained, quantified and ranked, and the optimal choices for each management technique were identified.

  1. Evaluation of biological nematicides on the control of root-knot nematode (Meloidogyne incognita Chitwood at protected crops house in tomato (Lycopersicon esculentum Mill.

    Directory of Open Access Journals (Sweden)

    Pedro Fidel Fuentes Chaviano

    2011-06-01

    Full Text Available This research was carried out on Tomato (Lycopersicon esculentum Mill. variety HA 3105 at the protected crops houses in Empresa Azucarera Melanio Hernández of Sancti Spiritus, located at the southwest of Tuinucú, from November 2009 to May 2010 with the objective of evaluating the effectiveness of biological nematicides on the control of the root-knot nematode, Meloidogyne incognita Chitwood. Three treatments were used: Agrocelhone, biological nematicide HeberNem and HeberNem + Trichoderma. Biological nematicides showed better results than Agrocelhone regarding the length of the leaf, the height of the plant, the yield of tomato and the control of nematodes HeberNem showed the best results in the control of the nematode, decreasing the infestation from degree V to degree II.

  2. Tomato contact dermatitis

    DEFF Research Database (Denmark)

    Paulsen, Evy; Christensen, Lars P; Andersen, Klaus Ejner

    2012-01-01

    The tomato plant (Solanum lycopersicum) is an important crop worldwide. Whereas immediate-type reactions to tomato fruits are well known, contact dermatitis caused by tomatoes or tomato plants is rarely reported. The aims of this study were to present new data on contact sensitization to tomato...... plants and review the literature on contact dermatitis caused by both plants and fruits. An ether extract of tomato plants made as the original oleoresin plant extracts, was used in aimed patch testing, and between 2005 and 2011. 8 of 93 patients (9%) tested positive to the oleoresin extracts....... This prevalence is in accordance with the older literature that reports tomato plants as occasional sensitizers. The same applies to tomato fruits, which, in addition, may cause protein contact dermatitis. The allergens of the plant are unknown, but both heat-stable and heat-labile constituents seem...

  3. Three-year comparative study of polyphenol contents and antioxidant capacities in fruits of tomato (Lycopersicon esculentum Mill.) cultivars grown under organic and conventional conditions.

    Science.gov (United States)

    Anton, Dea; Matt, Darja; Pedastsaar, Priit; Bender, Ingrid; Kazimierczak, Renata; Roasto, Mati; Kaart, Tanel; Luik, Anne; Püssa, Tõnu

    2014-06-04

    In the present study, four tomato cultivars were grown under organic and conventional conditions in separate unheated greenhouses in three consecutive years. The objective was to assess the influence of the cultivation system on the content of individual polyphenols, total phenolics, and antioxidant capacity of tomatoes. The fruits were analyzed for total phenolic content by the Folin-Ciocalteau method and antioxidant capacity by the DPPH free radical scavenging assay. Individual phenolic compounds were analyzed using HPLC-DAD-MS/MS. Among 30 identified and quantified polyphenols, significantly higher contents of apigenin acetylhexoside, caffeic acid hexoside I, and phloretin dihexoside were found in all organic samples. The content of polyphenols was more dependent on year and cultivar than on cultivation conditions. Generally, the cultivation system had minor impact on polyphenols content, and only a few compounds were influenced by the mode of cultivation in all tested cultivars during all three years.

  4. Assessment of Total Phenolic and Flavonoid Content, Antioxidant Properties, and Yield of Aeroponically and Conventionally Grown Leafy Vegetables and Fruit Crops: A Comparative Study

    Science.gov (United States)

    Chandra, Suman; Khan, Shabana; Avula, Bharathi; Lata, Hemant; Yang, Min Hye; ElSohly, Mahmoud A.; Khan, Ikhlas A.

    2014-01-01

    A comparison of the product yield, total phenolics, total flavonoids, and antioxidant properties was done in different leafy vegetables/herbs (basil, chard, parsley, and red kale) and fruit crops (bell pepper, cherry tomatoes, cucumber, and squash) grown in aeroponic growing systems (AG) and in the field (FG). An average increase of about 19%, 8%, 65%, 21%, 53%, 35%, 7%, and 50% in the yield was recorded for basil, chard, red kale, parsley, bell pepper, cherry tomatoes, cucumber, and squash, respectively, when grown in aeroponic systems, compared to that grown in the soil. Antioxidant properties of AG and FG crops were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DDPH) and cellular antioxidant (CAA) assays. In general, the study shows that the plants grown in the aeroponic system had a higher yield and comparable phenolics, flavonoids, and antioxidant properties as compared to those grown in the soil. PMID:24782905

  5. Rootstock-mediated changes in xylem ionic and hormonal status are correlated with delayed leaf senescence, and increased leaf area and crop productivity in salinized tomato.

    Science.gov (United States)

    Albacete, Alfonso; Martínez-Andújar, Cristina; Ghanem, Michel Edmond; Acosta, Manuel; Sánchez-Bravo, José; Asins, María J; Cuartero, Jesús; Lutts, Stanley; Dodd, Ian C; Pérez-Alfocea, Francisco

    2009-07-01

    Tomato crop productivity under salinity can be improved by grafting cultivars onto salt-tolerant wild relatives, thus mediating the supply of root-derived ionic and hormonal factors that regulate leaf area and senescence. A tomato cultivar was grafted onto rootstocks from a population of recombinant inbred lines (RILs) derived from a Solanum lycopersicum x Solanum cheesmaniae cross and cultivated under moderate salinity (75 mM NaCl). Concentrations of Na(+), K(+) and several phytohormones [abscisic acid (ABA); the cytokinins (CKs) zeatin, Z; zeatin riboside, ZR; and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC)] were analysed in leaf xylem sap in graft combinations of contrasting vigour. Scion leaf area correlated with photosystem II (PSII) efficiency (F(v)/F(m)) and determined fruit productivity. Xylem K(+) (but not Na(+)), K(+)/Na(+), the active CK Z, the ratio with its storage form Z/ZR and especially the ratio between CKs and ACC (Z/ACC and Z + ZR/ACC) were positively loaded into the first principal component (PC) determining both leaf growth and PSII efficiency. In contrast, the ratio ACC/ABA was negatively correlated with leaf biomass. Although the underlying physiological mechanisms by which rootstocks mediate leaf area or chlorophyll fluorescence (and thus influence tomato salt tolerance) seem complex, a putative potassium-CK interaction involved in regulating both processes merits further attention.

  6. Tolerância da cultura do tomate à salinidade do solo em ambiente protegido Tolerance of tomato crop to salinity of soil in protected ambient

    Directory of Open Access Journals (Sweden)

    Pedro R. F. Medeiros

    2012-01-01

    Full Text Available Com o intuito de contribuir com os dados disponíveis na literatura sobre tolerância das culturas à salinidade do solo oriunda de sais fertilizantes, o presente trabalho tem, como objetivo, determinar a tolerância da cultura do tomate a salinidade do solo, a partir de dois manejos de fertirrigação e seis níveis iniciais de salinidade (1,0; 2,0; 3,0; 4,0; 5,0 e 6,0 dS m-1 a partir das variáveis produção e componentes de produção, em ambiente protegido com solo franco-argiloso. O experimento foi realizado no Departamento de Engenharia de Biossistemas da ESALQ/USP, Piracicaba,SP. O delineamento estatístico foi aleatorizado em blocos, com 4 repetições, concluindo que a produção e os componentes de produção da cultura do tomate foram afetados estatisticamente, tanto pelo tipo de manejo de fertirrigação quanto pelos níveis de salinidade do solo, com redução na tolerância da cultura do tomate, passando a ser classificada sensível à salinidade do solo.In order to contribute to data available in the literature on tolerance of crops to soil salinity derived from fertilizer salts, the present study aims to determine the tolerance of tomato crop to the soil salinity, based on two managements of fertirrigation and six initial levels of salinity (1.0; 2.0; 3.0; 4.0; 5.0 and 6.0 dS m-1 from the variables production and components of production, in a protected environment with sandy-clay soil. The experiment was conducted at the Department of Biosystems Engineering of ESALQ/USP, Piracicaba/SP. The experimental design was in randomized blocks, with 4 repetitions. Concluding that the production and the components of production of tomato crop were significantly affected both by the type of management of fertirrigation and by the levels of soil salinity, with a reduction in the tolerance of tomato crop, being classified as sensitive to soil salinity.

  7. Single basal application of thiacloprid for the integrated management of Meloidogyne incognita and Bemisia tabaci in tomato crops

    Science.gov (United States)

    Dong, Sa; Ren, Xiaofen; Zhang, Dianli; Ji, Xiaoxue; Wang, Kaiyun; Qiao, Kang

    2017-01-01

    Tomato growers commonly face heavy nematode (Meloidogyne incognita) and whitefly (B-biotype Bemisia tabaci) infestations, and previous studies demonstrated that thiacloprid could be used to control M. incognita and B. tabaci in cucumber. However, the efficacy of a single basal application of thiacloprid to control both pests and its effect on yield in tomato remains unknown. In this study, the potential of thiacloprid application to the soil for the integrated control of M. incognita and B. tabaci in tomato was evaluated in the laboratory and the field. Laboratory tests showed that thiacloprid was highly toxic to whitefly adults and eggs with an average lethal concentration 50 (LC50) of 14.7 and 62.2 mg ai L−1, respectively, and the LC50 of thiacloprid for nematode J2s and eggs averaged 36.2 and 70.4 mg ai L−1, respectively. In field trials, when thiacloprid was applied to the soil at 7.5, 15 and 30 kg ha−1 in two consecutive years, whitefly adults decreased by 37.8–75.4% within 60 days of treatment, and the root-galling index was reduced by 31.8–85.2%. Optimum tomato plant growth and maximum yields were observed in the 15 kg ha−1 treatment. The results indicated that a single basal application of thiacloprid could control M. incognita and B. tabaci and enhance tomato growth and yield. PMID:28120937

  8. Single basal application of thiacloprid for the integrated management of Meloidogyne incognita and Bemisia tabaci in tomato crops

    Science.gov (United States)

    Dong, Sa; Ren, Xiaofen; Zhang, Dianli; Ji, Xiaoxue; Wang, Kaiyun; Qiao, Kang

    2017-01-01

    Tomato growers commonly face heavy nematode (Meloidogyne incognita) and whitefly (B-biotype Bemisia tabaci) infestations, and previous studies demonstrated that thiacloprid could be used to control M. incognita and B. tabaci in cucumber. However, the efficacy of a single basal application of thiacloprid to control both pests and its effect on yield in tomato remains unknown. In this study, the potential of thiacloprid application to the soil for the integrated control of M. incognita and B. tabaci in tomato was evaluated in the laboratory and the field. Laboratory tests showed that thiacloprid was highly toxic to whitefly adults and eggs with an average lethal concentration 50 (LC50) of 14.7 and 62.2 mg ai L‑1, respectively, and the LC50 of thiacloprid for nematode J2s and eggs averaged 36.2 and 70.4 mg ai L‑1, respectively. In field trials, when thiacloprid was applied to the soil at 7.5, 15 and 30 kg ha‑1 in two consecutive years, whitefly adults decreased by 37.8–75.4% within 60 days of treatment, and the root-galling index was reduced by 31.8–85.2%. Optimum tomato plant growth and maximum yields were observed in the 15 kg ha‑1 treatment. The results indicated that a single basal application of thiacloprid could control M. incognita and B. tabaci and enhance tomato growth and yield.

  9. Comparison of GHG fluxes from conventional and energy crop production from adjacent fields in the UK, using novel technologies

    Science.gov (United States)

    Keane, James Benjamin; Ineson, Phil; Toet, Sylvia; Stockdale, James; Vallack, Harry; Blei, Emanuel; Bentley, Mark; Howarth, Steve

    2016-04-01

    With combustion of fossil fuels driving anthropogenic climate change, allied to a diminishing global reserve of these resources it is vital for alternative sources of energy production to be investigated. One alternative is biomass; ethanol fermented from corn (Zea mays) or sugar cane (Saccharum spp.) has long been used as a petroleum substitute, and oilseed rape (OSR, Brassica napus) is the principal feedstock for biodiesel production in Germany, the third biggest producer of this fuel globally. Diverting food crops into energy production would seem counter-productive, given there exists genuine concern regarding our ability to meet future global food demand, thus attention has turned to utilising lignocellulosic material: woody tissue and non-food crop by-products such as corn stover. For this reason species such as the perennial grass Miscanthus (Miscanthus x giganteus) are being cultivated for energy production, and these are referred to as second generation energy crops. They are attractive since they do not deplete food supplies, have high yields, require less fertiliser input than annual arable crops, and can be grown on marginal agricultural land. To assess the effectiveness of a crop for bioenergy production, it is vital that accurate quantification of greenhouse gas (GHG) fluxes is obtained for their cultivation in the field. We will present data from a series of studies investigating the GHG fluxes from the energy crops OSR and Miscanthus under various nutrient additions in a comparison with conventional arable cropping at the same site in the United Kingdom (UK). A combination of methods were employed to measure fluxes of CO2, CH4 and N2O from both soil and vegetation, at various temporal and spatial scales. Conventional manual chambers were deployed on a monthly regime to quantify soil GHG fluxes, and were supplemented with automated soil flux chambers measuring soil respiration at an hourly frequency. Additionally, two novel automated chamber systems

  10. Broccoli Cultivar Performance under Organic and Conventional Management Systems and Implications for Crop Improvement

    NARCIS (Netherlands)

    Renaud, E.N.C.; Lammerts Van Bueren, E.; Caldas Paulo, M.J.; Eeuwijk, van F.A.; Juvik, J.A.; Hutton, M.G.; Myers, J.R.

    2014-01-01

    To determine if present commercial broccoli cultivars meet the diverse needs of organic management systems, such as adaptation to low N input, mechanical weed management, and no chemical pesticide use, and to propose the selection environments for crop improvement for organic production, we compared

  11. Susceptibility of geographically isolated populations of the Tomato red spider mite (Tetranychus evansi Baker & Pritchard to commonly used acaricides on tomato crops in Kenya

    Directory of Open Access Journals (Sweden)

    F. J. Toroitich

    2014-04-01

    Full Text Available Farmers in Kenya continue to raise concerns of difficulty in managing Tetranychus evansi, the most widespread pest species of tomato applying the most commonly used acaricides. This invasive pest species is not only found in Kenya, but in Eastern and Southern Africa, as well as parts of Europe and Asia. In the current study, populations of T. evansi were collected from farms in the four major tomato-growing areas of Kenya (Loitoktok, Kibwezi, Athi-River and Subukia and their susceptibility compared to a laboratory culture (ICIPE that had been maintained for three years without exposure to acaricides. Susceptibility of T. evansi eggs and adults (contact and residual to Brigade (bifenthrin, Dimethoate (dimethoate, Karate (lambdacyhalothrin, Kelthane (dicofol, Omite (propargite and Polytrin (profenofos+ cypermethrin was tested in the laboratory using respective manufacturer’s recommended concentrations. Dimethoate resulted in variable ovicidal mortality while Kelthane, Brigade, Karate, Omite and Polytrin had high mortality across all populations. Similarly, adult contact and residual mortality was lower than that of the other chemicals when exposed to Dimethoate regardless of the location. Furthermore, it also had no residual effect on the mites from ICIPE and Kibwezi. On the other hand, Kelthane was most lethal against the mites from all locations followed by Brigade and Polytrin in that order. Omite caused significantly lower mortality on mites from Subukia while Karate produced variable effects on mites from Kibwezi, Loitoktok and Subukia. The implications of these findings are further discussed.

  12. Role of epigeic earthworms on trophic group of nematodes during organic matter decomposition in litter bags under tomato cropping on ultisol

    Science.gov (United States)

    Alam, Syamsu; Lisnawati, Kilowasid, Laode Muhammad Harjoni; Darwis, Asniah, Nurmas, Andi

    2015-09-01

    Epigeic earthworms are often used to restore of soil quality. Trophic group of nematodes plays an important role in driving of decomposition rate of organic matter. Ultisols is characterized with the soil biological quality that is not suitable for the development of vegetable crops. The objective of this study was to analyze the effect of epigeic earthworms on the abundance of nematode trophic groups during the decomposition of organic material in litter bags under cropping of tomato (L. esculentum Mill.) on Ultisols. Epigeic species of earthworms (Lumbricus sp.) were used to modify the soil environment. The experiment treatment consisted of nine combinations of three types of organic matter and three individual levels of earthworms. The organic material consisted of litters of C. odorata, I. cylindrica and Colopogonium sp. The number of earthworms consisted of 0, 20 and 40 individuals plot-1. Each combination of each litter type and number of earthworms was repeated three times in an experimental randomized block design. Research found three trophic groups of nematodes, namely root-herbivorous, bacterivorous and predaceous in the litter bags. Abundance of root-herbivorous between combinations was significantly different at 30 days after exposure. Abundance of bacterivorous nematodes among treatments was significant at 60 days after exposure, which at the 30 and 90 days were not significant. Abundance of predaceous was differed significantly at the 60 and 90 days, and at the 30 days was not significantly different. Constant of decomposition rate of each organic matter under different number of earthworms was similar. Coefficient correlation showed that relation between the constant of decomposition rate with abundance of root-herbivorous was positive at 30 days and negative with bacterivorous at the 90 days. Research concluded that the introduction of epigeic earthworms influenced trophic group dynamics of nematodes during the decomposition of organic material

  13. The effects of PLA biodegradable and polypropylene nonwoven crop mulches on selected components of tomato grown in the field

    OpenAIRE

    Zawiska Izabela; Siwek Piotr

    2014-01-01

    The results of two years (2010-2011) of field studies using two types of nonwoven mulches (one biodegradable, polylactic acid PLA 54 g m-2, and traditional polypropylene PP 50 g m-2) on the yield and quality of tomato are presented. Seeds of tomato (‘Mundi’ F1) were sown in a greenhouse, in containers filled with perlite and sand, and then the plants at the cotyledon stage were replanted in multipot trays filled with substrate for vegetable plants. In the last week of May, seedlings were plan...

  14. Yield and Quality of Sequentially Grown Cherry Tomato and Lettuce under Long-Term Conventional, Low-Input and Organic Soil Management Systems

    NARCIS (Netherlands)

    Moccia, S.; Chiesa, A.; Oberti, A.; Tittonell, P.A.

    2006-01-01

    Besides to conventional fertiliser use, organic and low-input technologies are being increasingly used for soil management in vegetable production. However, different factors operating during crop growth (i.e. the pre-harvest factors) and related to soil properties may affect yield and quality of ve

  15. [Arachnofauna (araneae: Araneae) in transgenic and conventional cotton crops (Gossypium hirsutum) in the North of Santa Fe, Argentina].

    Science.gov (United States)

    Almada, Melina Soledad; Sosa, María Ana; González, Alda

    2012-06-01

    Spiders have considerable potential importance for their role as predators to some pests in agricultural systems. The composition of spiders in transgenic and conventional cotton at the Research Station of INTA Reconquista (Santa Fe) was studied during the 2005-2006 season. The experiment was a complete randomized block design with three replications and three treatments: transgenic Bt cotton (ALBt), conventional cotton without chemical control (ALCSC), and conventional cotton with chemical control (ALCCC). Weekly, spiders were collected using nets, vertical cloth and pitfall-traps. A total of 1255 specimens (16 families, and 32 species) were collected. Seven families were found in all the treatments, mainly Thomisidae (n=1 51, 84.04%) and Araneidae (n=83, 6.64%). The Hunting spiders guild ambushers (n=1053, 83.91%), "Orb weavers" (n=85, 6.77%) and "Stalkers" (n=53, 4.22%) were more abundant. There were no significant differences in the indexes diversity between treatments. Spiders were presented during the whole crop season, with peaks about flowering and boll maturity, with the highest abundance in ALBt. This work is part of the first set of data registered in Argentina about spider's community in cotton crops.

  16. Comparison of Soil Respiration in Typical Conventional and New Alternative Cereal Cropping Systems on the North China Plain

    Science.gov (United States)

    Gao, Bing; Ju, Xiaotang; Su, Fang; Gao, Fengbin; Cao, Qingsen; Oenema, Oene; Christie, Peter; Chen, Xinping; Zhang, Fusuo

    2013-01-01

    We monitored soil respiration (Rs), soil temperature (T) and volumetric water content (VWC%) over four years in one typical conventional and four alternative cropping systems to understand Rs in different cropping systems with their respective management practices and environmental conditions. The control was conventional double-cropping system (winter wheat and summer maize in one year - Con.W/M). Four alternative cropping systems were designed with optimum water and N management, i.e. optimized winter wheat and summer maize (Opt.W/M), three harvests every two years (first year, winter wheat and summer maize or soybean; second year, fallow then spring maize - W/M-M and W/S-M), and single spring maize per year (M). Our results show that Rs responded mainly to the seasonal variation in T but was also greatly affected by straw return, root growth and soil moisture changes under different cropping systems. The mean seasonal CO2 emissions in Con.W/M were 16.8 and 15.1 Mg CO2 ha−1 for summer maize and winter wheat, respectively, without straw return. They increased significantly by 26 and 35% in Opt.W/M, respectively, with straw return. Under the new alternative cropping systems with straw return, W/M-M showed similar Rs to Opt.W/M, but total CO2 emissions of W/S-M decreased sharply relative to Opt.W/M when soybean was planted to replace summer maize. Total CO2 emissions expressed as the complete rotation cycles of W/S-M, Con.W/M and M treatments were not significantly different. Seasonal CO2 emissions were significantly correlated with the sum of carbon inputs of straw return from the previous season and the aboveground biomass in the current season, which explained 60% of seasonal CO2 emissions. T and VWC% explained up to 65% of Rs using the exponential-power and double exponential models, and the impacts of tillage and straw return must therefore be considered for accurate modeling of Rs in this geographical region. PMID:24278340

  17. Neural network-based crop growth model to predict processing tomato yield on a site-specific basis using remotely sensed data

    Science.gov (United States)

    Koller, Michal

    Remote sensing is one of the major data acquisition tools available to rapidly acquire soil and plant related information over a wide area for use in precision agriculture. Green canopy has very specific reflectance characteristics distinguishing it from other materials such as soil and dry vegetative matter. Reflectance values in red (R) and near infra-red (NIR) spectral bands have been widely used for calculating normalized difference vegetation index (NDVI). Many researchers have related NDVI values to plant vigor, water stress, leaf area index (LAI) and/or yield. However, vegetative indices such as NDVI are usually sensitive to background reflectance characteristics. Often soil adjusted vegetation indices (SAVI) are used to minimize the background effect. In this study we have developed a relationship between the processing tomato yield and SAVI based on the R and NIR reflectance. Eight three band (R, NIR and green) aerial images were obtained at approximately two-week intervals during the 2000 processing tomato growing season. These images were analyzed to obtain SAVI values which were in turn related to LAI using regression techniques. A tuned neural network was developed to predict daily LAI values based on the biweekly experimental LAI values derived from aerial images. The coefficients of multiple determination between the actual LAI and neural network predicted LAI values were greater than 0.96 for all 56 grid points. The LAI values were numerically integrated over the whole growing season to obtain cumulative leaf area index days (CLAID). The CLAID values predicted from the neural network correlated very well with experimentally derived CLAID values (coefficient of determination, r2 = 0.83) indicating that the neural network model simulated processing tomato growth well. A crop growth model that was capable of predicting crop yield based on neural network predicted LAI values and CIMIS weather data was developed. Although predicted yield tended to be low

  18. The role of weeds in the spread of Tomato spotted wilt virus by thrips tabaci (Thysanoptera: Thripidae) in tobacco crops

    NARCIS (Netherlands)

    Chatzivassiliou, E.K.; Peters, D.; Katis, N.I.

    2007-01-01

    Oviposition of Thrips tabaci, larval development and their potential to acquire Tomato spotted wilt virus (TSWV) from infected Amaranthus retroflexus, Datura stramonium, Lactuca serriola, Solanum nigrum and Sonchus oleraceus plants and the ability of the adults to transmit this virus to these weeds

  19. The effects of PLA biodegradable and polypropylene nonwoven crop mulches on selected components of tomato grown in the field

    Directory of Open Access Journals (Sweden)

    Zawiska Izabela

    2014-12-01

    Full Text Available The results of two years (2010-2011 of field studies using two types of nonwoven mulches (one biodegradable, polylactic acid PLA 54 g m-2, and traditional polypropylene PP 50 g m-2 on the yield and quality of tomato are presented. Seeds of tomato (‘Mundi’ F1 were sown in a greenhouse, in containers filled with perlite and sand, and then the plants at the cotyledon stage were replanted in multipot trays filled with substrate for vegetable plants. In the last week of May, seedlings were planted on mulches in the field at a spacing of 50 × 100 cm. The mulch was maintained throughout the growing season. A plot that remained unmulched served as the control. Tomatoes were harvested once a week. The fruits were evaluated for L-ascorbic acid, dry matter, soluble sugars and nitrate content. In 2011, the analysis of the plant material showed that the concentration of L-ascorbic acid was about 23% higher in the tomato fruits harvested from plants grown on biodegradable PLA 61 g m-2 mulch in comparison to the control. A similar effect was demonstrated for the soluble sugar concentration in 2011 for both types of nonwovens.

  20. Decreased functional diversity and biological pest control in conventional compared to organic crop fields.

    Science.gov (United States)

    Krauss, Jochen; Gallenberger, Iris; Steffan-Dewenter, Ingolf

    2011-01-01

    Organic farming is one of the most successful agri-environmental schemes, as humans benefit from high quality food, farmers from higher prices for their products and it often successfully protects biodiversity. However there is little knowledge if organic farming also increases ecosystem services like pest control. We assessed 30 triticale fields (15 organic vs. 15 conventional) and recorded vascular plants, pollinators, aphids and their predators. Further, five conventional fields which were treated with insecticides were compared with 10 non-treated conventional fields. Organic fields had five times higher plant species richness and about twenty times higher pollinator species richness compared to conventional fields. Abundance of pollinators was even more than one-hundred times higher on organic fields. In contrast, the abundance of cereal aphids was five times lower in organic fields, while predator abundances were three times higher and predator-prey ratios twenty times higher in organic fields, indicating a significantly higher potential for biological pest control in organic fields. Insecticide treatment in conventional fields had only a short-term effect on aphid densities while later in the season aphid abundances were even higher and predator abundances lower in treated compared to untreated conventional fields. Our data indicate that insecticide treatment kept aphid predators at low abundances throughout the season, thereby significantly reducing top-down control of aphid populations. Plant and pollinator species richness as well as predator abundances and predator-prey ratios were higher at field edges compared to field centres, highlighting the importance of field edges for ecosystem services. In conclusion organic farming increases biodiversity, including important functional groups like plants, pollinators and predators which enhance natural pest control. Preventative insecticide application in conventional fields has only short-term effects on aphid

  1. The Effects of Endogenous Mycorrhiza (Glomus spp. on Stand Establishment Rate and Yield of Open Field Tomato Crop

    Directory of Open Access Journals (Sweden)

    ANGJELINA VUKSANI

    2015-03-01

    Full Text Available Graded seeds of a tomato commercial cultivar (Suta F1 were sown in plugged (30 cm3 foam trays filled with vermiculate. For the half of seeds the substrate was homogenously mixed (10% vol/vol with broken expanded clay biologically activated by immobilized propagules of naturally occurring AM-fungi (Glomus intradadices, Glomus etunicatum, Glomus mosseae, Glomus geosporum, Glomus clarum, supplied by BioSym B.V. For the next half, it was homogenously mixed with free clay particles of the same size with the previous one (10% vol/vol. Thirty days after sowing, tomato seedlings were transplanted in to 200 cm3 plastic pots filled with vermiculite. Either AMF non pre inoculated seedlings (non AMF, or AMF pre inoculated (AMF, were equally divided in three subgroups based on the level of salinity applied in the nutrient solution (0, 50 and 100 mM sodium chlorate. On the day of transplanting and 7 days after transplanting, 10 plants were randomly selected for each experimental plot. For each of them, the dry weight of root (RW, leaf (LA, steam (SW and the whole plant (W was determined and based on that, the relative growth rates (RGR and root relative growth rate (RRGR were calculated and used as the parameters assessing the stand establishment rate of tomato seedlings after transplanting. Additionally, stomata conductance and leaf temperature were measured to assess AMF effects on physiological performance of transplanted seedlings. In order to assess the effect of AM fungi pre inoculation on total yield, parallel with pot transplanting, a field experiment was conducted according to a three replication randomized block design. It was proved that the production of open field tomato can be enhanced by the pre inoculation of AM fungi to tomato transplants at the nursery stage. Due to the pre inoculation of AM fungi, the tomato seedlings can subsequently benefit a faster stand establishment rate and higher vigor which is further reflected to a prolonged

  2. Response of soil microbial biomass and community structures to conventional and organic farming systems under identical crop rotations.

    Science.gov (United States)

    Esperschütz, Jürgen; Gattinger, Andreas; Mäder, Paul; Schloter, Michael; Fliessbach, Andreas

    2007-07-01

    In this study the influence of different farming systems on microbial community structure was analyzed using soil samples from the DOK long-term field experiment in Switzerland, which comprises organic (BIODYN and BIOORG) and conventional (CONFYM and CONMIN) farming systems as well as an unfertilized control (NOFERT). We examined microbial communities in winter wheat plots at two different points in the crop rotation (after potatoes and after maize). Employing extended polar lipid analysis up to 244 different phospholipid fatty acids (PLFA) and phospholipid ether lipids (PLEL) were detected. Higher concentrations of PLFA and PLEL in BIODYN and BIOORG indicated a significant influence of organic agriculture on microbial biomass. Farmyard manure (FYM) application consistently revealed the strongest, and the preceding crop the weakest, influence on domain-specific biomass, diversity indices and microbial community structures. Esterlinked PLFA from slowly growing bacteria (k-strategists) showed the strongest responses to long-term organic fertilization. Although the highest fungal biomass was found in the two organic systems of the DOK field trial, their contribution to the differentiation of community structures according to the management regime was relatively low. Prokaryotic communities responded most strongly to either conventional or organic farming management.

  3. Production and efficiency of water usage in capsicum crops under no-tillage and conventional planting systems

    Directory of Open Access Journals (Sweden)

    Maria Eliani Holanda Coelho

    Full Text Available This study aimed to evaluate the effect of no-tillage and conventional planting systems, and of weed-management strategies on water-usage efficiency in capsicum crops. The experiment was carried out at the Universidade Federal Rural do Semi-Árido in Mossoró, Rio Grande do Norte, using a split-plot layout in a randomized block design with four replications. The tillage systems were evaluated in the plots, and three weed-management strategies evaluated in the subplots (soil cover with polyethylene film, and with and without weeds. The density and dry mass of the weeds, the commercial and total productivity, and the daily water consumption were all evaluated. It was found that the no-tillage system reduced the density and dry mass of the weeds in comparison to conventional systems, and the interference of these plants reduced commercial productivity under both planting systems. The strategy of weeds under a no-tillage system, despite a higher water consumption, showed a productivity and efficiency of water usage superior to those of the strategies of polyethylene film both under no-tillage and conventional systems, and of weeds under a conventional tillage system.

  4. Functional and nutritional characteristics of soft wheat grown in no-till and conventional cropping systems

    Science.gov (United States)

    The effects of no-till vs. conventional farming practices were evaluated on soft wheat functional and nutritional characteristics, including kernel physical properties, whole wheat composition, antioxidant activity and end-product quality. Soft white winter wheat cv. ORCF 102 was evaluated over a tw...

  5. The 1981-1982 NCLAN (National Crop Loss Assessment Network) program at LLNL: The effects of ozone and sulfur dioxide, singly and in combination, on field-grown tomato

    Energy Technology Data Exchange (ETDEWEB)

    Surano, K.A.; Mutters, R.G.; Temple, P.J.; Bingham, G.E.; Kercher, J.R.; Shinn, J.H.

    1987-07-01

    The combined effects of ozone (O/sub 3/) and sulfur dioxide (SO/sub 2/) exposures on tomato (Lycopersicon esculentum Mill.) crop yields were measured during the 1981 and 1982 growing seasons. The effort, part of the National Crop Loss Assessment Network (NCLAN), had the objective of determining the yield dose response of tomato grown under commercial conditions. Dose response was determined using step-wise multiple regression techniques and was expressed as reduction in marketable yield as a function of increased pollutant levels. The crops were harvested during mid-September 1981 and during mid-October 1982. No O/sub 3/- or So/sub 2/-induced visible injury symptons (lesions, chlorosis, or becrosis) were apparent on foliage or fruits. Total marketable fruit fresh weight was used as the dependent variable in the regression analysis because it most closely represented the economic value of the crop. Comparison of chambered- and companion-plot data showed that the chambers themselves caused significant effects on the crop. Yields were reduced 12% in 1981 and 24% in 1982 due to the chambers. There was also more unripened fruit within the chambers because of chamber-induced uneven distribution of an aerially-applied ripening agent. Dose-response results were independent of the chamber effects. Based on companion-plot data, the yield of the crop in the research area was 65/plus minus/2 Mg ha/sup /minus/1/ in both years. The research-area yields correlated well with those from the adjacent commercial plantation. With respect to tomato production in the San Joaquin Valley, we recommend that an air quality management strategy be adopted to contain ambient O/sub 3/ concentrations at or below present levels. Containment at present levels would avoid O/sub 3/-induced tomato yield reductions greater than 7%, even during atypical years such as 1982. 26 refs., 17 figs., 9 tabs.

  6. Efficacy of an alphabaculovirus-based biological insecticide for control of Chrysodeixis chalcites (Lepidoptera: Noctuidae) on tomato and banana crops.

    Science.gov (United States)

    Simón, Oihane; Bernal, Alexandra; Williams, Trevor; Carnero, Aurelio; Hernández-Suárez, Estrella; Muñoz, Delia; Caballero, Primitivo

    2015-12-01

    Chrysodeixis chalcites (Esper) is a major pest of tomato in Mediterranean countries and attacks banana in the Canary Islands (Spain). The efficacy of Chrysodeixis chalcites single nucleopolyhedrovirus (ChchSNPV-TF1) was evaluated in plant growth chambers and greenhouse trials performed on tomato and banana plants respectively. Treatments were applied using a compressed air sprayer. Mean (± SE) lethal infection varied from 77 ± 10% to 94 ± 3% in second-instar larvae fed for 2 days on tomato plants treated with 2 × 10(6) to 5 × 10(7) virus occlusion bodies (OBs) L(-1) , increasing to ∼100% infection after 7 days. Mortality of larvae collected from banana at different intervals post-application varied from 54 ± 10% to 96 ± 4% in treatments involving 1 × 10(8) -1 × 10(9) OBs L(-1) , whereas indoxacarb (Steward 30% WG) and Bacillus thuringiensis var. kurstaki (Biobit 16% WP) treatments produced between 22 ± 6% and 32 ± 5% pest mortality. All treatments significantly reduced plant defoliation compared with untreated controls. Application of 1 × 10(9) OBs L(-1) was 3-4-fold more effective than chemical or B. thuringiensis treatments. Larvae acquired lethal infection more rapidly when feeding on tomato than banana plants, but this difference disappeared following >60 min of feeding. This information should prove useful in the registration of ChchSNPV-TF1 as a bioinsecticide in the Canary Islands and Europe. © 2014 Society of Chemical Industry.

  7. Changes in physico-chemical properties of soil by adding organic amendments in a tomato crop; Cambios en la propiedades fisico-quimicas del suelo por adicion de enmiendas organicas en cultivo de tomate

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez Navarro, A.; Marin Salneandro, P.; Delgado Iniesta, M. J.

    2009-07-01

    This study possible changes in the physico-chemical properties of soil under intensive cultivation of tomatoes after the addition of two different types of organic amendments: a natural as sheep manure and synthetic made. Trial plots that were designed are located in the NE of the province of Granada, in Puebla de Trial plots that were designed are located in the NE of the province of Granada, in Puebla de Don Fadrique, in the are that in recent years, change are very important in agriculture, from traditional farms extensive cultivation of rain-fed cereal crops such as intensive vegetale broccoli or tomatoes. (Author) 16 refs.

  8. Effects of Crop Sanitation and Ridomil MZ Applications on Late Blight Severity and Tomato Yields in Cameroon

    Directory of Open Access Journals (Sweden)

    Younyi, PC.

    2004-01-01

    Full Text Available Tomato (Lycopersicon esculentum production in Cameroon is usually handicapped by late blight caused by Phytophthora infestans. A field trial was conducted during 1997 in Dschang, Cameroon, to assess the effect of Ridomil MZ (8% metalaxyl + 64% mancozeb sprays, and sanitation (a weekly picking of diseased leaves on late blight development and yield of five tomato varieties. Plots received Ridomil MZ (2.5 kg/ha and sanitation singly or combined. Control plots were neither sprayed nor cleaned from diseased leaves. All treatments were applied ten times in a weekly schedule. Late blight intensity was assessed every 7 days and marketable fruit yields were obtained at maturity. Differences in late blight intensity between sanitation and control plots were not significant (P= 0,05. Fungicide treatments were more effective than sanitation in reducing late blight severity. Percent fruit infection was 100% in control or sanitation plots of ARP I366-1, ARP D1, ARP D2, Roma, and no marketable fruits were harvested on these treatments. Late blight was less severe on Mecline compared to the other varieties. Consequently, Mecline out-yielded Roma, ARP I366-1, ARP D1 and ARP D2 varieties. Results suggest that the fungicide-alternative method of late blight control, using sanitation is not as effective in tomato late blight management as appropriate fungicide sprays.

  9. Yield and apparent dry matter and nitrogen balances for muskmelon in a long-term comparison between an organic and a conventional low input cropping system

    Directory of Open Access Journals (Sweden)

    Michela Farneselli

    2015-09-01

    Full Text Available Nine-year yields and apparent balances of dry matter and nitrogen (N are reported for muskmelon cultivated in a long-term comparison trial between an organic and a conventional low input system in Central Italy. In every year, yield, above ground biomass and N accumulation of each cash crop, green manure and weeds, and the partitioning between marketable yield and crop residues were determined. Apparent dry matter and nitrogen balances were calculated at the end of each crop cycle by taking into account the amounts of dry matter and ex novo N supplied to the system as green manure legume Ndfa (i.e., an estimate of N derived from the atmosphere via symbiotic fixation and fertilisers, and those removed with marketable yield. Differences between systems varied across years. On average, organic muskmelon yielded 16% less than the conventional one, while the fruit quality was similar in the two cropping systems. Fruit ripening began one week later and it was more scaled than in the crop grown conventionally. This was the consequence of a slow initial growth of the organic crop, due to inadequate green manure N total supply or timing of N release. Moreover such a wide spaced crop (0.5 plants m–2, in rows 2 m apart was not efficient in intercepting N released from green manure biomass incorporated broadcast. Compared to the conventional crop management, the organic crop management resulted in much higher organic matter supply to the soil and in higher residual N after harvest. Thus, the choice of cultivating wheat just after melon to prevent postharvest residual N loss appears a key strategy especially in organic systems. Fall-winter green manure crops contributed to the self-sufficiency of the organic system by supplying muskmelon with either N absorbed from the soil or ex novo legume Ndfa.

  10. CAPTURE OF Tuta absoluta (Meyrick (LEPIDOPTERA: GELECHIIDAE WITH LIGHT TRAP IN TOMATO CROP CAPTURA DE Tuta absoluta (Meyrick (LEPIDOPTERA: GELECHIIDAE COM ARMADILHA LUMINOSA NA CULTURA DO TOMATEIRO TUTORADO

    Directory of Open Access Journals (Sweden)

    Aline Cavalcante Rodrigues de Oliveira

    2008-09-01

    Full Text Available

    The tomato leafminer (Tuta absoluta represents a serious problem for the tomato crop, not only due to the intensity of its attack, but also to its occurrence during all the crop cycle. In Brazil, this pest has been controlled almost exclusively with insecticides, what is undesirable for economic and environmental reasons. In order to get more information on the control of this pest this research was carried out, in Ouro Verde (Goiás State, Brazil, from September through October 2002, to evaluate the use of light  traps for capture of adult T. absoluta specimens. The treatments used were: 1 black lamp; 2 BLB lamp; 3 Grolux lamp; and 4 fluorescent daylight lamp. The experimental design used was randomized complete blocks with four replications. The parameter to evaluate treatment capture efficiency was the counting of the adult T. absoluta specimens trapped. The results showed that the BLB and ultraviolet lamps were the most efficient treatments. Therefore, both can help to control the tomato leafminer in integrated pest management programs.

    KEY-WORDS: Tomato pinworm; integrated pest management; tomato.

    A traça-do-tomateiro (Tuta absoluta representa um sério problema à tomaticultura, não somente pela intensidade de ataque, mas também por sua ocorrência durante todo o ciclo da cultura. No Brasil, esta praga tem sido controlada, quase exclusivamente, com inseticidas, o que é indesejável, tanto por motivos econômicos, quanto ambientais. Visando a obter maiores informa

  11. Control of the Tomato Leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), in Open-Field Tomatoes by Indigenous Natural Enemies Occurring in Israel.

    Science.gov (United States)

    Shaltiel-Harpaz, Liora; Gerling, Dan; Graph, Shaul; Kedoshim, Hendrika; Azolay, Lotem; Rozenberg, Tamir; Nachache, Yaakov; Steinberg, Shimon; Allouche, Arnon; Alon, Tamar

    2016-02-01

    The tomato leafminer, Tuta absoluta (Meyrick), had established in Israel by 2010, attacking both open-field tomatoes and greenhouse crops.We searched for its natural enemies in open-field tomatoes, and tried to determine their potential for controlling this pest. We surveyed the local natural enemies in open tomato fields and measured their impact on pest populations in an unsprayed field. We assessed the suppressive ability of the dominant hemipteran predator, Nesidiocoris tenuis Reuter, against T. absoluta under controlled laboratory conditions and evaluated the impact of its augmentation on T. absoluta control in open-field tomatoes. We found five natural enemy species:the predator, N. tenuis, two braconids, and two eulophids. Predation accounted for 64.5±9.2% (mean ± SE) of T. absoluta larval mortality, whereas parasitism accounted for 20.96±7.5%. Together, they eliminated the pest population at tomato harvest time. Under controlled conditions, predation by N. tenuis rose from 58 to 72% with increased density of T. absoluta, suggesting positive density dependence. The reduction of T. absoluta (83%) by N. tenuis was higher than that of Bemisia tabaci (32%), suggesting a preference of N. tenuis for T. absoluta. Augmentation of N.tenuis was as effective as conventional treatment insecticide treatment, and plant damage was low and did not seem to affect yield. Results indicate that reduced pesticide use enables indigenous natural enemies, particularly N.tenuis, to successfully control T. absoluta and prevent crop damage in open-field tomatoes.

  12. Accumulation and Translocation of Essential and Nonessential Elements by Tomato Plants (Solanum lycopersicum) Cultivated in Open-Air Plots under Organic or Conventional Farming Techniques.

    Science.gov (United States)

    Liñero, Olaia; Cidad, Maite; Carrero, Jose Antonio; Nguyen, Christophe; de Diego, Alberto

    2015-11-04

    A 5-month experiment was performed to study the accumulation of several inorganic elements in tomato plants cultivated using organic or synthetic fertilizer. Plants were harvested in triplicate at six sampling dates during their life cycle. Statistical and chemometric analysis of data indicated the sequestration of toxic elements and of Na, Zn, Fe, and Co in roots, while the rest of the elements, including Cd, were mainly translocated to aboveground organs. A general decreasing trend in element concentrations with time was observed for most of them. A negative correlation between some element concentrations and ripening stage of fruits was identified. Conventionally grown plants seemed to accumulate more Cd and Tl in their tissues, while organic ones were richer in some nutrients. However, there was no clear effect of the fertilizer used (organic vs synthetic) on the elemental composition of fruits.

  13. Quantitative losses in mechanized harvesting corn crop in small footprint and conventional

    OpenAIRE

    Daniel Mariano Leite; Mauri Martins Teixeira; Haroldo Carlos Fernandes; Danilo Roberto Loureiro; Marcelo Marques Costa

    2012-01-01

    The objective of this study was to evaluate the quantitative losses occurred in the mechanical harvesting of the corn grown in conventional and reduced gaps. The experimental design was randomized blocks with three replications and treatments distributed in a factorial (2 x 3 x 3), and two spacings (0.45 m and 0.90 m), three speeds of the combine (1.8 km.h-1 3.5 km.h-1 and 4.1 km.h-1) and three openings of the concave (25 mm, 30 mm and 35 mm). For treatments where the spacing was 0.45 m, the ...

  14. Carbon Dynamics of Bioenergy Cropping Systems Compared to Conventional Cotton Cropping Systems in the Southern Cotton Belt Region of the U.S.

    Science.gov (United States)

    Rajan, N.; Sharma, S.; Casey, K.; Maas, S. J.

    2015-12-01

    We are facing an unprecedented challenge in securing America's energy future. To address this challenge, increased biofuel crop production is needed. Second-generation biofuels are made from the by-products of intensive agriculture or from less-intensive agriculture on more marginal lands. The Southwestern U.S. Cotton Belt can play a significant role in this effort through a change from more conventional crops (like continuous cotton) to second-generation biofuel feedstocks (biomass sorghum and perennial grasses). We have established eddy covariance flux towers in producer fields in the Southern High Plains region. Among the four land uses compared, the net carbon uptake was the highest for the biomass sorghum field. During the year 2014, the biomass sorghum field gained approximately 672 gC m-2y-1. The next highest carbon uptake was recorded for the Old World Bluestem grass field, which was approximately 301 gC m-2y-1. The dominant land use in the region is cotton. While the forage sorghum and grass fields acted as net carbon sinks, the irrigated cotton field acted as a net carbon source to the atmosphere during the same period. The irrigated cotton field exhibited a net carbon loss of approximately 246 gC m-2y-1. In contrast, the dryland cotton field acted as a net carbon sink, with a total uptake of approximately 58 g C m-2y-1. The net primary production of the irrigated cotton field was higher than that of the dryland cotton field, yet the irrigated field was a significant carbon source to the atmosphere. This was due to conventional tillage practices combined with irrigation which enhanced the ecosystem respiration significantly compared to the dryland field. In 2014, an early spring cold front caused poor germination of seeds in the majority of the cotton fields in the region, including the eddy covariance site. This site was re-planted on 9 June, which shortened the growing season for cotton. This was also a contributing factor to this field being a net

  15. Benefits and Costs of Biologically Contained Genetically Modified Tomatoes and Eggplants in Italy and Spain

    Directory of Open Access Journals (Sweden)

    Rolf A. Groeneveld

    2011-08-01

    Full Text Available In this paper we assess the benefits and costs of introducing biologically contained genetically modified (GM crops, with an application to the potential introduction of GM tomatoes and eggplants in Italy and Spain. Such crops possess both the standard beneficial GM traits, and they prevent introgression of transgenes from GM crops to their conventional or wild relatives, thereby adding to the safety of their cultivation. As a result, coexistence regulations for these crops are less stringent than for crops without biological containment. The potential adoption of biologically contained GM tomatoes and eggplants is assessed in a cost-benefit framework for Italy and Spain. We conclude that biological containment has considerable potential benefits if policy makers are willing to loosen the restrictions on the introduction of these varieties.

  16. Effects of reduced-rate methyl bromide applications under conventional and virtually impermeable plastic film in perennial crop field nurseries.

    Science.gov (United States)

    Hanson, Bradley D; Gerik, James S; Schneider, Sally M

    2010-08-01

    Producers of perennial crop nursery stock in California use preplant soil fumigation to meet state phytosanitary requirements. Although methyl bromide (MB) has been phased out in many agricultural industries, it is still the preferred treatment in the perennial nursery industry and is used under Critical Use Exemptions and Quarantine/Preshipment provisions of the Montreal Protocol. The present research was conducted to evaluate reduced-rate MB applications sealed with conventional and low-permeability plastic films compared with the primary alternative material. Reduced rates (100-260 kg ha(-1)) of MB applied in combination with chloropicrin (Pic) and sealed with a low-permeability plastic film provided weed and nematode control similar to the industry standard rate of 392 kg ha(-1) MB:Pic (98:2) sealed with high-density polyethylene (HDPE) film. However, the primary alternative chemical, 1,3-dichloropropene (1,3-D), tended to provide slightly lower pest control even on sites with relatively low plant parasitic nematode, soil-borne pathogen and weed pest pressure. If California regulations change to allow the use of low-permeability films in broadcast fumigant applications, the results of this research suggest that reduced rates of MB in perennial crop nurseries could serve as a bridge strategy until more technically, economically and environmentally acceptable alternatives are developed. Published 2010 by John Wiley & Sons, Ltd.

  17. Testing the Suitability of a Terrestrial 2D LiDAR Scanner for Canopy Characterization of Greenhouse Tomato Crops.

    Science.gov (United States)

    Llop, Jordi; Gil, Emilio; Llorens, Jordi; Miranda-Fuentes, Antonio; Gallart, Montserrat

    2016-09-06

    Canopy characterization is essential for pesticide dosage adjustment according to vegetation volume and density. It is especially important for fresh exportable vegetables like greenhouse tomatoes. These plants are thin and tall and are planted in pairs, which makes their characterization with electronic methods difficult. Therefore, the accuracy of the terrestrial 2D LiDAR sensor is evaluated for determining canopy parameters related to volume and density and established useful correlations between manual and electronic parameters for leaf area estimation. Experiments were performed in three commercial tomato greenhouses with a paired plantation system. In the electronic characterization, a LiDAR sensor scanned the plant pairs from both sides. The canopy height, canopy width, canopy volume, and leaf area were obtained. From these, other important parameters were calculated, like the tree row volume, leaf wall area, leaf area index, and leaf area density. Manual measurements were found to overestimate the parameters compared with the LiDAR sensor. The canopy volume estimated with the scanner was found to be reliable for estimating the canopy height, volume, and density. Moreover, the LiDAR scanner could assess the high variability in canopy density along rows and hence is an important tool for generating canopy maps.

  18. Evaluation of Low Pressure Fogging System for Improving Crop Yield of Tomato (Lycopersicon esculentum Mill.: Grown under Heat Stress Conditions

    Directory of Open Access Journals (Sweden)

    Kobi Shilo

    2013-06-01

    Full Text Available In Mediterranean regions, many tomato plants are grown throughout the hot summer period (May–September in sheltered cultivation, mainly for plant protection reasons. Most of the shelters that are used are low cost net houses covered with 50 mesh insect proof net. In most cases these net houses have a flat roof and no ventilation or climate control measures. This insufficient ventilation during the hot summer leads to above optimal air temperatures and causes moderate heat stress inside the shelters, which leads to yield reduction. The aim of this study was to evaluate the ability of a simple and inexpensive low pressure fogging system installed in a naturally ventilated net house to lower temperatures and improve the yield during the summer. The study showed that in areas where relative air humidity (RH during the daytime is less than 60%, tomato plants improved their performance when grown through the summer in net houses under moderate heat stress. Under fogging conditions pollen grain viability and fruit set were significantly improved. This improvement influenced the yield picked during September (104–136 DAP. However, total seasonal yield was not affected by the fogging treatment.

  19. 'BRS Tospodoro': a high lycopene processing tomato cultivar adapted to organic cropping systems and with multiple resistance to pathogens 'BRS Tospodoro': Cultivar de tomate para processamento com alto licopeno, adaptada aos sistemas de cultivo orgânico e com múltiplos genes de resistência a patógenos

    Directory of Open Access Journals (Sweden)

    Leonardo de B Giordano

    2010-06-01

    Full Text Available 'BRS Tospodoro' is a high lycopene tomato cultivar, which combines multiple disease resistance genes and desirable processing traits. This cultivar was found to be suitable for both conventional and organic crop systems. 'BRS Tospodoro' was obtained via backcross breeding using 'Viradoro' as recurrent parent and the inbred line 'CNPH 1306' as the donor of the Pto gene (resistance to Pseudomonas syringae pv. tomato race 0. 'BRS Tospodoro' has the Mi1-2 gene that controls resistance to root-knot nematodes (Meloidogyne incognita, M. javanica, and M. arenaria as well as tolerance to populations of the aphid Macrosiphum euphorbiae (vector of Potyvirus species, and to whiteflies (Bemisia tabaci. 'BRS Tospodoro' has also the Sw-5b gene, which controls resistance to major Tospovirus species (Groundnut ringspotirus, Tomato chlorotic spot virus, Chrysanthemum stem necrosis virus, and Tomato spotted wilt virus. This cultivar is also resistant to Stemphylium solani and S. lycopersici (Sm gene, Fusarium oxysporum f. sp. lycopersici race 1 (I-1 gene and Verticillium dahlie race 1 (Ve gene. 'BRS Tospodoro' presents uniform fruit ripening, with the first harvest being done around 110 to 120 days after plant emergence. The fruits are firm, square-shaped, with an average weight of 46 g (in organic cropping to above 70 g (in conventional cropping. The soluble solids content is in the range between 4.6 and 4.8 ºBrix. The external fruit color is uniform (gene u and bright red (L* = 44.1; a* = 33.9; b* = 20.4. The average lycopene content of mature fruits is 104 µg/g. This cultivar has the jointless locus (j2, which facilitates both manual and mechanical harvesting. 'BRS Tospodoro' has determinate growth habit (locus sp with vigorous foliage, which provides good fruit protection from sunscald. 'BRS Tospodoro' can be cultivated in all the traditional processing tomato-producing areas of Brazil without the need of any technical adjustment. 'BRS Tospodoro' displayed

  20. Optimal model-based deficit irrigation scheduling using AquaCrop: a simulation study with cotton, potato and tomato

    DEFF Research Database (Denmark)

    Linker, Raphael; Ioslovich, Ilya; Sylaios, Georgios

    2016-01-01

    variables are the irrigation amounts for each day of the season. The objective function is the expected yield calculated with the use of a model. In the present work we solved this optimization problem for three crops modeled by the model AquaCrop. This optimization problem is non-trivial due to the non......-smooth behavior of the objective function and the fact that it involves multiple integer variables. We developed an optimization scheme for generating sub-optimal irrigation schedules that take implicitly into account the response of the crop to water stress, and used these as initial guesses for a full...... should use an irrigation schedule that maximizes the yield and abides to the quota constraints. In contrast to the widespread use of irrigation scheduling based on agronomy practices, irrigation scheduling may be considered as a constrained optimization problem. When drip irrigation is used, the decision...

  1. Physiological activity and material production in processing tomato under continuous cropping%连作对加工番茄植株生理活性和物质生产的影响

    Institute of Scientific and Technical Information of China (English)

    康亚龙; 刘彦荣; 刘建国; 李梦格; 郝梦超; 蒋桂英

    2015-01-01

    AbstractProcessing tomato production is the main business of the “red industry” in Xinjiang Uygur Autonomous Region. But with regional distribution and industrial development and the resulting in continuous cropping obstacle, the sustainable development of local processing tomato production has been severely restricted. A field experiment was conducted in 2013 in the Experimental Station of Shihezi Agricultural University to study the effects of continuous cropping on physiological activity and material production of processing tomato plant using the ‘Ligeer 87-5’ tomato variety. In the experiment, three treatments of continuous cropping (3 years, 5 years and 7 years) of processing tomato were set up. In addition, the first-planting of processing tomato was used as the control. The results showed that continuous cropping obstacles appeared in the whole growth period of tomato after 3 years of continuous cropping. The problems significantly increased with increased years of continuous cropping. Peroxidase activity initially increased and later decreased with increasing years of continuous cropping. While catalase activity, superoxide dismutase activity and root activity steadily decreased, malondialdehyde content increased significantly with increasing years of continuous cropping. The effects of continuous cropping on both antioxidant enzymes activities and malonidaldehyde content were greater on tomato roots than on leaves. Tomato dry matter production decreased with increasing years of continuous cropping. Dry weight was significantly lower in the 7-year continuous cropping treatment than in the control treatment. Continuous cropping reduced translocation of storage materials, rate of transfer and rate of contribution respectively by 57.3%, 21% and 35.6%. At flowering and fruit development stages, dry matter production decreased with increasing years of continuous cropping, eventually reducing tomato yield. 3, 5 and 7 years of continuous cropping reduced

  2. Effect of some detergents, humate, and composition of seedbed on crop of tomato plants in a hydroponic culture

    Science.gov (United States)

    Guminka, A. Z.; Gracz-Nalepka, M.; Lukasiewicz, B.; Sobolewicz, E.; Turkiewicz, I. T.

    1978-01-01

    It is established that single detergent doses distinctly stimulate vegetative development of plants in the initial stage when humates are available. When detergents are applied every four weeks in a hydroponic culture, in which the seedbed does not contain active humates, the crop is reduced by 50%. This adverse effect does not occur when the seedbed is a mixture of brown coal and peat.

  3. DES-TOMATO: A Knowledge Exploration System Focused On Tomato Species

    KAUST Repository

    Salhi, Adil

    2017-07-14

    Tomato is the most economically important horticultural crop used as a model to study plant biology and particularly fruit development. Knowledge obtained from tomato research initiated improvements in tomato and, being transferrable to other such economically important crops, has led to a surge of tomato-related research and published literature. We developed DES-TOMATO knowledgebase (KB) for exploration of information related to tomato. Information exploration is enabled through terms from 26 dictionaries and combination of these terms. To illustrate the utility of DES-TOMATO, we provide several examples how one can efficiently use this KB to retrieve known or potentially novel information. DES-TOMATO is free for academic and nonprofit users and can be accessed at http://cbrc.kaust.edu.sa/des_tomato/, using any of the mainstream web browsers, including Firefox, Safari and Chrome.

  4. Microorganismos benéficos como biofertilizantes eficientes para el cultivo del tomate (Lycopersicon esculentum, Mill Beneficial microorganisms as efficient biofertilisers for tomato crops (Lycopersicon esculentum, Mill

    Directory of Open Access Journals (Sweden)

    Hernández Annia

    2005-12-01

    Full Text Available En el uso y manejo de biofertilizantes en la agricultura, uno de los principales problemas es el desconocimiento de las especies presentes en los agroecosistemas y en la rizosfera de los cultivos, para su posible utilización eficiente. Desde el punto de vista ecológico, es importante conocer los integrantes de la comunidad bacteriana que favorecen su aplicación como inoculantes y propician un efecto agrobiológico positivo en los cultivos agrícolas. Esta investigación se desarrolló con el objetivo de evaluar la efectividad agrobiológica de Azospirillum sp, en el crecimiento, desarrollo y rendimiento en el cultivo del tomate. Para ello, se partió de seleccionar el género microbiano predominante en la rizosfera del cultivo y posteriormente se evaluó el efecto de su inoculación a partir de la respuesta del cultivo. Los resultados demostraron que los géneros Pseudomonas, Azospirillum, Azotobacter, Bacillus y Streptomyces, forman parte de la comunidad microbiana de la rizosfera del tomate, en las condiciones estudiadas, y que Azospirillum es el género dominante. La inoculación artificial de esta rizobacteria causó un efecto positivo sobre el crecimiento de las plántulas, así como en el estado nutricional de las plantas, con un rendimiento agrícola superior a un 11 % con respecto a las plantas testigo. Se obtuvo un alto nivel poblacional en la rizosfera de las plantas inoculadas. Palabras clave: rizosfera, inoculante, crecimiento, rendimiento.One of the main problems regarding the efficient use and management of biofertilizers in agriculture lies in the unknown species present in agro-ecosystems and crop rhizospheres. From the ecological point of view, it is important to know the members of the bacterial population allowing them to be applied as inoculants and enable a positive agro-biological effect on agricultural crops. This investigation was aimed at evaluating the agro-biological effectiveness of Azospirillum sp. on tomato

  5. Produtividade do tomateiro em diferentes substratos e modelos de casas de vegetação Tomato crop production under different substrates and greenhouse models

    Directory of Open Access Journals (Sweden)

    Osmar A. Carrijo

    2004-03-01

    Full Text Available Um experimento com a cultura do tomate, foi instalado na Embrapa Hortaliças em Brasília, durante os anos de 2000 e 2001, para avaliar a produção do tomateiro em diferentes substratos e casas de vegetação. Os substratos utilizados foram casca de arroz, casca de arroz parcialmente carbonizada, fibra de coco verde, lã de rocha, maravalha, serragem e substrato para produção de mudas utilizado na Embrapa Hortaliças (150 L de terra de subsolo, 50 L de casca de arroz parcialmente carbonizada e 17 L de esterco de galinha. Os modelos de casas de vegetação utilizados foram teto em arco, arco com teto convectivo e capela. Não foi verificada diferença estatística significativa quanto a produção de frutos comerciais entre os substratos fibra de coco (10,4 kg m-2, serragem (9,3 kg m-2, casca de arroz carbonizada (9,3 kg m-2 e maravalha (9,0 kg m-2. A menor produção foi obtida com o substrato lã de rocha (6,4 kg m-2. Houve redução da produção entre os anos de cultivo, em torno de 33%, em decorrência de um intenso ataque de traça do tomateiro (Tuta absoluta em todas as casas de vegetação, prejudicando a produtividade. O maior peso médio dos frutos foi obtido sobre a fibra de coco (128 g m-2 e casca de arroz carbonizada (123 g m-2, independente do modelo de casa de vegetação utilizado.The trial was carried out at Embrapa Hortaliças, in Brasilia, Brazil, to evaluate the performance of tomato crop production during two years (2000 and 2001, under three greenhouse models and different types of substrates. The greenhouse models were arch roof; even span and an arch roof with upper convective aperture. The substrates were rice husk, carbonized rice husk, coconut fiber, sawdust, coarsed sawdust, rockwool and a substrate for seedling production used at Embrapa Hortaliças. No significant statistical difference was verified for tomatoes cultivated in coconut fiber (10,4 kg m-2, sawdust (9,9 kg m-2, carbonized rice husk (9,3 kg m-2 and

  6. The Impact of Broccoli II & Tomato II on European patents in conventional breeding, GMO’s and Synthetic Biology:

    DEFF Research Database (Denmark)

    Minssen, Timo; Nordberg, Ana

    2015-01-01

    biological processes are – at least in principle- patentable under the European Patent Convention (EPC). This decision leaves considerable leeway for patenting novel and inventive plants and products thereof, which have been produced by methods including breeding steps, as opposed to plant varieties...

  7. SWOT Analysis on Breeding R&D; of Tropical Crops in China in the Context of Implementing UPOV Convention 1991 Act

    Institute of Scientific and Technical Information of China (English)

    Wenhui; PU; Dingchao; DONG; Qiong; LI; Huijian; ZHANG

    2015-01-01

    This paper firstly reviewed development process of UPOV Conventions and briefly introduced current situation of protection of new tropical crop varieties in China. Using SWOT approach,it analyzed internal strengths,weaknesses,external opportunities and threats of breeding R&D; of tropical crops if implementing UPOV Convention 1991 Act in China. Internal strengths include rich resources,strategic significance of tropical crops,solid crop breeding foundation,and broad market prospect. Internal weaknesses include insufficient reserve of germplasm resources,weak R&D; and protection of new varieties,low market level of breeding R&D; entities,unreasonable structure,low input in breeding R&D;,and weak protection awareness. Opportunities include bringing into play strengths of rich tropical plant resources,stimulating enthusiasm for new variety R&D; and awareness for variety protection,promoting structural optimization of breeding input entities,raising technology level of breeding R&D;,and promoting international cooperation of new variety protection. Threats include more strict restriction of intellectual property right,impact to weak fields of new variety R&D;,and difficulty in implementing limitation of farmer privileges. Finally,it came up with four recommendations for breeding R&D; of tropical crops in China in the contest of implementing UPOV Convention 1991 Act.

  8. Quantitative proteomics by 2DE and MALDI MS/MS uncover the effects of organic and conventional cropping methods on vegetable products

    DEFF Research Database (Denmark)

    Nawrocki, Arkadiusz; Thorup-Kristensen, Kristian; Jensen, O.N.

    2011-01-01

    Organic farming aims to be environmentally sound, but the question as to whether organic cropping brings more nutritional benefits to farmers and consumers than the conventional cropping needs still to be answered. To gain insights into the molecular effects of organic farming we used proteome...... short handling times of fresh plant materials. In the case of cabbage samples, the abundance levels of 58 out of more than 1300 quantified protein spots varied significantly between conventional farming and any of the organic cropping systems. Proteome profiles were also very similar between carrot root...... samples, where 68 out of 1800 resolved protein spots varied significantly. Proteins of the glycolytic pathway and Krebs cycle as well as several proteins related to amino acid and protein metabolism were overexpressed in organically farmed cabbage. Proteins related to detoxification processes were...

  9. Evapotranspiração e coeficiente de cultivo do tomate caqui cultivado em ambiente protegido Evapotranspiration and crop coefficient of Kaki tomato cultivated in greenhouse

    Directory of Open Access Journals (Sweden)

    Ligia S. Reis

    2009-06-01

    Full Text Available Os parâmetros aerodinâmicos de uma cultura cultivada em ambiente protegido podem ser considerados dependentes do nível de radiação global, temperatura do ar e umidade do ar, com base em leis exponenciais. Assim sendo se propôs com este trabalho, estimar a evapotranspiração e o coeficiente de cultivo da cultura do tomate caqui em ambiente protegido, sob irrigação por gotejamento, utilizando-se o modelo de Penman-Monteith. Os parâmetros aerodinâmicos foram medidos com sensores conectados à estação automática instalada dentro do ambiente protegido. A evapotranspiração da cultura (ETc foi determinada experimentalmente por meio de lisímetros de drenagem e a umidade do solo foi medida através de sensores instalados a uma profundidade de 20 cm. O desempenho do modelo de Penman-Monteith foi comparado aos valores decendiais do balanço hídrico nos lisímetros; já a evapotranspiração de referência foi calculada com dados externos e utilizada para o cálculo do Kc da cultura; enfim, os resultados indicaram que o modelo de Penman-Monteith subestima os valores de evapotranspiração encontrados pelo balanço hídrico nos lisímetros.The aerodynamic parameters of a crop cultivated in greenhouse can be considered dependent upon the level of global radiation, air temperature and relative humidity, based on exponential laws. Consequently, this work intends to estimate the evapotranspiration and the crop coefficient of Kaki tomato in greenhouse, under drip irrigation, using the Penman-Monteith model. The aerodynamic parameters were measured with sensors connected to the automatic station installed inside the greenhouse. The ETc was determined experimentally through drainage lysimeters and the soil water content was measured through sensors installed at a depth of 20 cm. The performance of the Penman-Monteith model was compared to decennial values of the water balance in the lysimeters, while the reference evapotranspiration was calculated

  10. Avaliação de técnica de tomaticultura em gramado (TEG na amazônia oriental Evaluation of TEG (grassland tomato cropping technique in Eastern Amazon, Brazil

    Directory of Open Access Journals (Sweden)

    Simon S. Cheng

    2002-06-01

    Full Text Available No Brasil, o tomateiro é cultivado principalmente em época de baixa precipitação e temperaturas amenas como as regiões Sudeste, Nordeste e Centro Oeste. Na Amazônia Oriental, uma região tropical úmida de baixa altitude, com temperatura elevada, a cultura do tomateiro enfrenta muitas dificuldades. A baixa produtividade e a mortalidade das plantas têm reduzido o número de produtores desta hortaliça, com a produção local de tomate inferior a 0,1% do volume comercializado anualmente. A técnica de Tomaticultura Em Gramado (TEG foi concebida em 1997 e aperfeiçoada em 1998, para solucionar os problemas da tomaticultura sob clima quente e chuvoso da Amazônia, usando dois principais mecanismos: 1. Campo gramado para controlar a proliferação de doenças, pragas, plantas invasoras, erosão e lixiviação de nutrientes. 2. Queima de descarte de madeira na área de cultivo para eliminar a grama e reduzir a acidez e pobreza do solo. O presente trabalho apresenta resultados preliminares de tomaticultura sob essa nova técnica, com avaliação de produtividade, ciclo da planta e peso médio do fruto. A nova técnica de cultivo permitiu a produtividade de 5,6 ± 0,2 kg por planta, sendo o período de colheita de 60 a 80 dias. A TEG demonstrou ser uma alternativa promissora para o sistema de produção de tomate na região quente e chuvosa, usando a gramínea como protetora e limpadora do solo.In Brazil, tomatoes are cultivated principally in seasons with low precipitation and cool temperature such as in regions of Southeast, Northeast and Central west. In East Amazon, a humid tropical region of low altitude with high temperature, tomato cropping faces many difficulties. Low yield and plant mortality had reduced the number of tomato growers, with annual local production less than 0.1% of total volume commercialized. The grassland tomato cropping technique (TEG was elaborated in 1997 and improved in 1998 to solve the problems of tomato production

  11. Comparative morphological analysis of cherry tomato fruits from three cropping systems Análise morfológica comparativa de frutos tomate cereja provenientes de três sistemas de cultivo

    Directory of Open Access Journals (Sweden)

    Sônia Cachoeira Stertz

    2005-06-01

    Full Text Available Attempts to meet produces market demand are not always followed by research reports showing the impact of novel, intensive cropping systems on the environment, human and animal health, and eventual chemical and structural changes of plants. This work carries a comparative evaluation of the morphology and anatomy of cherry tomato fruits obtained from organic, conventional and hydroponic cropping systems. Fruits were collected at the free market in the greater Curitiba, Paraná, Brazil. For each culture method, 50 fruits their weight, diameter and volume measured. For the anatomical analysis, samples of the pericarp of ten fruits were excised and embedded in glycol methacrylate; 6-mm cuts were then obtained, stained with toluidine blue, and mounted on permanent glass slides. The anatomical analysis and illustrations were performed in Olympus and Zeiss photonic microscope. Samples obtained from the organic cropping system presented the highest mass, diameter, volume and density. The pericarp of fruits, obtained from the conventional cropping system presented larger but less abundant cells in comparison to the pericarp of the hydroponic and organic-produced fruits.A ciência agrícola buscou novas formas de cultivo para atender às exigências de mercado e dividiu-se em três grandes vertentes: os sistemas de cultivo orgânico, convencional e hidropônico. Todavia, esses avanços nem sempre foram acompanhados por pesquisas que objetivassem apontar o impacto de uma nova técnica agrícola sobre o ambiente, a saúde humana e animal e aos próprios vegetais em seus aspectos químicos e estruturais. Este trabalho tem por objetivo o estudo comparativo da morfo-anatomia do fruto do Lycopersicon esculentum var. cerasiforme (Duval A. Gray, cultivado nos sistemas orgânico, convencional e hidropônico. Os frutos foram coletados no comércio varejista da Região Metropolitana de Curitiba, Paraná, Brasil. Para cada cultivo foram analisados 50 frutos. Cada

  12. Effect of rotating different leaf vegetables with tomato in plastic tunnel on reuse of continuous cropping substrate%轮作叶菜对大棚番茄连作基质重复利用效果的影响

    Institute of Scientific and Technical Information of China (English)

    李威; 孟焕文; 程智慧; 周静; 柴喜荣; 梁静

    2012-01-01

    【Objective】The study was done to compare the effects of rotating different kinds of leaf vegetables in continuous cropping organic substrate on reducing or eliminating the continuous cropping obstacle of tomato under plastic tunnel cultivation.【Method】Green garlic,no-heading Chinese cabbage and leaf lettuce were respectively planted in the substrate with two season continuous production of tomato during winter fallow season under plastic tunnel.Tomato was planted as the aftercrop to investigate the effects of leaf vegetable rotation taking winter fallow substrate as CK1,the fresh substrate of the same formula as CK2 and the fresh substrate with peat and perlite as CK3.【Result】Except for the obvious change in EC value for the substrate of continuous cropping treatment,there is little alteration in EC value,pH value,bulk density and total porosity in the substrates of the other treatments before and after crop cultivation.That means those substrates are relatively stable in physical and chemical properties which are all within the optimal range for tomato growing and are appropriate for reuse to grow tomato.The contents of alkali hydrolysable nitrogen,available phosphorus and available potassium in substrate of different treatments are decreased by different degrees after rotating of different leaf vegetables.The plant growth and root vigor of aftercrop tomato during the whole growth period in treatment A and B show significant predominance to CK1 and close to or not significantly different from CK2 and CK3.The yield of tomato in treatment rotated with green garlic shows no significant difference from CK3 but significantly higher than CK2.The effect of rotating no-heading Chinese cabbage is just next to rotating green garlic.However,winter fallow(CK1) presents the lowest yield.As for fruit quality,the contents of soluble sugar,soluble protein,lycopene and sugar-acid ratio are higher in tomato rotated with garlic which show no significant difference

  13. Tomato (Lycopersicon esculentum, cv CXD271BIO) yield and quality during converion from conventional to organic production

    OpenAIRE

    Nardo, N.; Foddai, MS; E.Azzini; Baiamonte, I; Di Ferdinando, S; Paoletti, S.; Vizioli, V; Paoletti, F.

    2011-01-01

    The conversion of a conventional farm to the methods of the organic farming is a very critical stage of technical and economical investment. From an agronomical point of view, usually the conversion needs a very different length of time with respect to that set by the regulation, because the organic farming is based on the soil fertility and, in general, on the preservation at the equilibrium of the farm’s ecosystem. These elements affect time and method of the conversions that, although base...

  14. Pesticide residue concentration in soil following conventional and Low-Input Crop Management in a Mediterranean agro-ecosystem, in Central Greece.

    Science.gov (United States)

    Karasali, Helen; Marousopoulou, Anna; Machera, Kyriaki

    2016-01-15

    The present study was focused on the comparative evaluation of pesticide residues, determined in soil samples from Kopaida region, Greece before and after the implementation of Low-Input Crop Management (LCM) protocols. LCM has been suggested as an environmental friendly plant protection approach to be applied on crops growing in vulnerable to pollution ecosystems, with special focus on the site specific problems. In the case of the specific pilot area, the vulnerability was mainly related to the pollution of water bodies from agrochemicals attributed to diffuse pollution primarily from herbicides and secondarily from insecticides. A total of sixty-six soil samples, were collected and analyzed during a three-year monitoring study and the results of the determined pesticide residues were considered for the impact evaluation of applied plant protection methodology. The LCM was developed and applied in the main crops growing in the pilot area i.e. cotton, maize and industrial tomato. Herbicides active ingredients such as ethalfluralin, trifluralin, pendimethalin, S-metolachlor and fluometuron were detected in most samples at various concentrations. Ethalfluralin, which was the active ingredient present in the majority of the samples ranged from 0.01 μg g(-1) to 0.26 μg g(-1) soil dry weight. However, the amount of herbicides measured after the implementation of LCM for two cropping periods, was reduced by more than 75% in all cases. The method of analysis was based on the simultaneous extraction of the target compounds by mechanical shaking, followed by liquid chromatography mass spectrometric and gas chromatography electron capture (LC-MS/MS and GC-ECD) analysis.

  15. The effect of transitional organic production practices on soilborne pests of tomato in a simulated microplot study.

    Science.gov (United States)

    Chellemi, Dan O; Rosskopf, Erin N; Kokalis-Burelle, Nancy

    2013-08-01

    The perceived risk of pest resurgence upon transition from conventional to organic-based farming systems remains a critical obstacle to expanding organic vegetable production, particularly where chemical fumigants have provided soilborne pest and disease control. Microplots were used to study the effects of soil amendments and cropping sequences applied over a 2-year transitional period from conventional to organic tomato (Solanum lycopersicum) cultivation on the incidence of bacterial wilt caused by Ralstonia solanacearum, purple nutsedge (Cyperus rotundus) reproduction, root galling by Meloidogyne incognita, and soil nematode populations. A continuation of tomato monoculture during the transitional period resulted in a disease incidence of 33%, as compared with 9% in microplots that were rotated with sunn hemp (Crotalaria juncea) and Japanese millet (Echinochloa crusgalli var. frumentacea). The benefits of disease control from a crop rotation extended into to a second season of organic tomato cultivation season, where bacterial wilt declined from 40% in microplots with a tomato monoculture to 17% in plots with a crop rotation sequence. Combining applications of urban plant debris with a continued tomato monoculture increased the incidence of bacterial wilt to 60%. During the transition period, tomato plants following a cover crop regime also had significantly lower levels of root galling from root-knot nematode infection compared with plants in the continuous tomato monoculture. Nutsedge tuber production was significantly increased in plots amended with broiler litter but not urban plant debris. Compared with a continuous monoculture, the results illustrate the importance of a systems-based approach to implementing transitional organic practices that is cognizant of their interactive effects on resident soilborne disease, weed, and pest complexes.

  16. Genotype-environment interaction and analysis of yield stability in trial with tomato crop in drought condition

    Directory of Open Access Journals (Sweden)

    Tony Boicet Fabre

    2016-02-01

    Full Text Available The present work was developed in the UBPC “The Coast”, belonging to the Miscellaneous Crops Enterprise of Campechuela municipality, Granma province, during the period from November to March of the year 2010 – 2011. It was carried out in a reddish brown fersialitic soil, according with the new genetic classification of the soils of Cuba MINAGRI, (2000, with the objective of studying and evaluating the influence of different organic fertilizers in the growth, development and yield in the cultivation of onion, variety Texas Early Grain. For this reason it was necessary to work on blocks with 4 treatments and 4 replies. The treatments applied were: treatment 1 (vermicompost, treatment 2 (cow dung, treatment 3 (cachaça and treatment 4 (control, those were applied in a solid way. The growth variables that were evaluated were number of leaves, height of the plant, equatorial diameter, polar diameter and fresh weight of the bulb. The yield was also evaluated in t.ha -1 per treatment. High yields are achieved with the application of organic fertilizer, among 27 and 30 t.ha -1, this is an alternative for the sustainable production of this product which is highly demanded by the population.

  17. Influences of Biodynamic and Conventional Farming Systems on Quality of Potato (Solanum Tuberosum L. Crops: Results from Multivariate Analyses of Two Long-Term Field Trials in Sweden

    Directory of Open Access Journals (Sweden)

    Lars Kjellenberg

    2015-09-01

    Full Text Available The aim of this paper was to present results from two long term field experiments comparing potato samples from conventional farming systems with samples from biodynamic farming systems. The principal component analyses (PCA, consistently exhibited differences between potato samples from the two farming systems. According to the PCA, potato samples treated with inorganic fertilizers exhibited a variation positively related to amounts of crude protein, yield, cooking or tissue discoloration and extract decomposition. Potato samples treated according to biodynamic principles, with composted cow manure, were more positively related to traits such as Quality- and EAA-indices, dry matter content, taste quality, relative proportion of pure protein and biocrystallization value. Distinctions between years, crop rotation and cultivars used were sometimes more significant than differences between manuring systems. Grown after barley the potato crop exhibited better quality traits compared to when grown after ley in both the conventional and the biodynamic farming system.

  18. Bio-energy Alliance High-Tonnage Bio-energy Crop Production and Conversion into Conventional Fuels

    Energy Technology Data Exchange (ETDEWEB)

    Capareda, Sergio [Texas A & M Univ., College Station, TX (United States). Dept. of Biological & Agricultural Engineering; El-Halwagi, Mahmoud [Texas A & M Univ., College Station, TX (United States). Dept. of Chemical Engineering; Hall, Kenneth R. [Texas A & M Univ., College Station, TX (United States). Dept. of Chemical Engineering; Holtzapple, Mark [Texas A & M Univ., College Station, TX (United States). Dept. of Chemical Engineering; Searcy, Royce [Texas A & M Univ., College Station, TX (United States). Dept. of Biological & Agricultural Engineering; Thompson, Wayne H. [Texas A & M Univ., College Station, TX (United States). Dept. of Soil and Crop Sciences; Baltensperger, David [Texas A & M Univ., College Station, TX (United States). Dept. of Soil and Crop Sciences; Myatt, Robert [Texas A & M Univ., College Station, TX (United States). Dept. of Soil and Crop Sciences; Blumenthal, Jurg [Texas A & M Univ., College Station, TX (United States). Dept. of Soil and Crop Sciences

    2012-11-30

    Maintaining a predictable and sustainable supply of feedstock for bioenergy conversion is a major goal to facilitate the efficient transition to cellulosic biofuels. Our work provides insight into the complex interactions among agronomic, edaphic, and climatic factors that affect the sustainability of bioenergy crop yields. Our results provide science-based agronomic response measures that document how to better manage bioenergy sorghum production from planting to harvest. We show that harvest aids provide no significant benefit as a means to decrease harvest moisture or improve bioenergy yields. Our efforts to identify optimal seeding rates under varied edaphic and climatological conditions reinforce previous findings that sorghum is a resilient plant that can efficiently adapt to changing population pressures by decreasing or increasing the numbers of additional shoots or tillers – where optimal seeding rates for high biomass photoperiod sensitive sorghum is 60,000 to 70,000 seeds per acre and 100,000 to 120,000 seeds per acre for sweet varieties. Our varietal adaptability trials revealed that high biomass photoperiod sensitive energy sorghum consistently outperforms conventional photoperiod insensitive sweet sorghum and high biomass forage sorghum as the preferred bioenergy sorghum type, with combined theoretical yields of both cellulosic and fermentable water-soluble sugars producing an average yield of 1,035 gallons of EtOH per acre. Our nitrogen trials reveal that sweet sorghums produce ample amounts of water-soluble sugars with minimal increases in nitrogen inputs, and that excess nitrogen can affect minor increases in biomass yields and cellulosic sugars but decrease bioenergy quality by decreasing water-soluble sugar concentrations and increasing ash content, specifically when plant tissue nitrogen concentrations exceed 0.6 %, dry weight basis. Finally, through our growth and re-growth trials, we show that single-cut high biomass sorghum bioenergy yields

  19. A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence.

    Science.gov (United States)

    Escobar-Bravo, Rocío; Alba, Juan M; Pons, Clara; Granell, Antonio; Kant, Merijn R; Moriones, Enrique; Fernández-Muñoz, Rafael

    2016-01-01

    Whiteflies damage tomatoes mostly via the viruses they transmit. Cultivated tomatoes lack many of the resistances of their wild relatives. In order to increase protection to its major pest, the whitefly Bemisia tabaci and its transmitted Tomato Yellow Leaf Curl Virus (TYLCV), we introgressed a trichome-based resistance trait from the wild tomato Solanum pimpinellifolium into cultivated tomato, Solanum lycopersicum. The tomato backcross line BC5S2 contains acylsucrose-producing type-IV trichomes, unlike cultivated tomatoes, and exhibits increased, yet limited protection to whiteflies at early development stages. Treatment of young plants with methyl jasmonate (MeJA) resulted in a 60% increase in type-IV trichome density, acylsucrose production, and enhanced resistance to whiteflies, leading to 50% decrease in the virus disease incidence compared to cultivated tomato. Using transcriptomics, metabolite analysis, and insect bioassays we established the basis of this inducible resistance. We found that MeJA activated the expression of the genes involved in the biosynthesis of the defensive acylsugars in young BC5S2 plants leading to enhanced chemical defenses in their acquired type-IV trichomes. Our results show that not only constitutive but also these inducible defenses can be transferred from wild into cultivated crops to aid sustainable protection, suggesting that conventional breeding strategies provide a feasible alternative to increase pest resistance in tomato.

  20. A jasmonate-inducible defense trait transferred from wild into cultivated tomato establishes increased whitefly resistance and reduced viral disease incidence

    Directory of Open Access Journals (Sweden)

    Rocío Escobar-Bravo

    2016-11-01

    Full Text Available Whiteflies damage tomatoes mostly via the viruses they transmit. Cultivated tomatoes lack many of the resistances of their wild relatives. In order to increase protection to its major pest, the whitefly Bemisia tabaci and its transmitted Tomato Yellow Leaf Curl Virus (TYLCV, we introgressed a trichome-based resistance trait from the wild tomato Solanum pimpinellifolium into cultivated tomato, S. lycopersicum. The tomato backcross line BC5S2 contains acylsucrose-producing type-IV trichomes, unlike cultivated tomatoes, and exhibits increased, yet limited protection to whiteflies at early development stages. Treatment of young plants with methyl jasmonate (MeJA resulted in a 60% increase in type-IV trichome density, acylsucrose production, and enhanced resistance to whiteflies, leading to 50% decrease in the virus disease incidence compared to cultivated tomato. Using transcriptomics, metabolite analysis and insect bioassays we established the basis of this inducible resistance. We found that MeJA activated the expression of the genes involved in the biosynthesis of the defensive acylsugars in young BC5S2 plants leading to enhanced chemical defenses in their acquired type-IV trichomes. Our results show that not only constitutive but also these inducible defenses can be transferred from wild into cultivated crops to aid sustainable protection, suggesting that conventional breeding strategies provide a feasible alternative to increase pest resistance in tomato.

  1. A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence

    Science.gov (United States)

    Escobar-Bravo, Rocío; Alba, Juan M.; Pons, Clara; Granell, Antonio; Kant, Merijn R.; Moriones, Enrique; Fernández-Muñoz, Rafael

    2016-01-01

    Whiteflies damage tomatoes mostly via the viruses they transmit. Cultivated tomatoes lack many of the resistances of their wild relatives. In order to increase protection to its major pest, the whitefly Bemisia tabaci and its transmitted Tomato Yellow Leaf Curl Virus (TYLCV), we introgressed a trichome-based resistance trait from the wild tomato Solanum pimpinellifolium into cultivated tomato, Solanum lycopersicum. The tomato backcross line BC5S2 contains acylsucrose-producing type-IV trichomes, unlike cultivated tomatoes, and exhibits increased, yet limited protection to whiteflies at early development stages. Treatment of young plants with methyl jasmonate (MeJA) resulted in a 60% increase in type-IV trichome density, acylsucrose production, and enhanced resistance to whiteflies, leading to 50% decrease in the virus disease incidence compared to cultivated tomato. Using transcriptomics, metabolite analysis, and insect bioassays we established the basis of this inducible resistance. We found that MeJA activated the expression of the genes involved in the biosynthesis of the defensive acylsugars in young BC5S2 plants leading to enhanced chemical defenses in their acquired type-IV trichomes. Our results show that not only constitutive but also these inducible defenses can be transferred from wild into cultivated crops to aid sustainable protection, suggesting that conventional breeding strategies provide a feasible alternative to increase pest resistance in tomato. PMID:27920785

  2. Structural and functional effects of conventional and low pesticide input crop-protection programs on benthic macroinvertebrate communities in outdoor pond mesocosms.

    Science.gov (United States)

    Auber, Arnaud; Roucaute, Marc; Togola, Anne; Caquet, Thierry

    2011-11-01

    The impacts of current and alternative wheat crop protection programs were compared in outdoor pond mesocosms in a 10-month long study. Realistic exposure scenarios were built based upon the results of modelling of drift, drainage and runoff of pesticides successively applied under two environmental situations characteristics of drained soils of northern France. Each situation was associated to two crop protection programs ("Conventional" and "Low-input") differing in the nature of pesticides used, number of treatments and application rate. Both programs induced significant direct negative effects on various invertebrate groups. Bifenthrin and cyprodynil were identified as the main responsible for these effects in conventional and low-input program, respectively. Indirect effects were also demonstrated especially following treatments with cyprodynil. Litter breakdown was significantly reduced in all treated mesocosms as the functional consequence of the decrease in the abundance of shredders (asellids, Gammarus pulex) illustrating the link between structural and functional effects of pesticides on macroinvertebrate communities. Recovery was observed for many taxa before the end of the study but not for the most sensitive non mobile taxa such as G. pulex. No influence of the agropedoclimatic situation on the effects was shown, suggesting than the main impacts were associated to inputs from drift. The results confirm that the proposed low-input program was less hazardous than the conventional program but the observed structural and functional impact of the low-input program suggest that further improvement of alternative crop protection programs is still needed.

  3. Comparing net ecosystem carbon dioxide exchange at adjacent commercial bioenergy and conventional cropping systems in Lincolnshire, United Kingdom

    Science.gov (United States)

    Morrison, Ross; Brooks, Milo; Evans, Jonathan; Finch, Jon; Rowe, Rebecca; Rylett, Daniel; McNamara, Niall

    2016-04-01

    The conversion of agricultural land to bioenergy plantations represents one option in the national and global effort to reduce greenhouse gas emissions whilst meeting future energy demand. Despite an increase in the area of (e.g. perennial) bioenergy crops in the United Kingdom and elsewhere, the biophysical and biogeochemical impacts of large scale conversion of arable and other land cover types to bioenergy cropping systems remain poorly characterised and uncertain. Here, the results of four years of eddy covariance (EC) flux measurements of net ecosystem CO2 exchange (NEE) obtained at a commercial farm in Lincolnshire, United Kingdom (UK) are reported. CO2 flux measurements are presented and compared for arable crops (winter wheat, oilseed rape, spring barely) and plantations of the perennial biofuel crops Miscanthus x. giganteus (C4) and short rotation coppice (SRC) willow (Salix sp.,C3). Ecosystem light and temperature response functions were used to analyse and compare temporal trends and spatial variations in NEE across the three land covers. All three crops were net in situ sinks for atmospheric CO2 but were characterised by large temporal and between site variability in NEE. Environmental and biological controls driving the spatial and temporal variations in CO2 exchange processes, as well as the influences of land management, will be analysed and discussed.

  4. Comparative environmental impacts of glyphosate and conventional herbicides when used with glyphosate-tolerant and non-tolerant crops

    Energy Technology Data Exchange (ETDEWEB)

    Mamy, Laure, E-mail: laure.mamy@versailles.inra.f [INRA-AgroParisTech, UMR 1091 Environnement et Grandes Cultures, 78850 Thiverval-Grignon (France); Gabrielle, Benoit, E-mail: benoit.gabrielle@agroparistech.f [INRA-AgroParisTech, UMR 1091 Environnement et Grandes Cultures, 78850 Thiverval-Grignon (France); Barriuso, Enrique, E-mail: barriuso@grignon.inra.f [INRA-AgroParisTech, UMR 1091 Environnement et Grandes Cultures, 78850 Thiverval-Grignon (France)

    2010-10-15

    The introduction of glyphosate-tolerant (GT) crops is expected to mitigate the environmental contamination by herbicides because glyphosate is less persistent and toxic than the herbicides used on non-GT crops. Here, we compared the environmental balances of herbicide applications for both crop types in three French field trials. The dynamic of herbicides and their metabolites in soil, groundwater and air was simulated with PRZM model and compared to field measurements. The associated impacts were aggregated with toxicity potentials calculated with the fate and exposure model USES for several environmental endpoints. The impacts of GT systems were lower than those of non-GT systems, but the accumulation in soils of one glyphosate metabolite (aminomethylphosphonic acid) questions the sustainability of GT systems. The magnitude of the impacts depends on the rates and frequency of glyphosate application being highest for GT maize monoculture and lowest for combination of GT oilseed rape and non-GT sugarbeet crops. - The impacts of herbicide applications on glyphosate-tolerant crops could be higher than expected due to the accumulation of a metabolite of glyphosate in soils.

  5. Presence of potential allergy-related linear epitopes in novel proteins from conventional crops and the implication for the safety assessment of these crops with respect to the current testing of genetically modified crops

    NARCIS (Netherlands)

    Kleter, G.A.; Peijnenburg, A.A.C.M.

    2003-01-01

    Mitochondria of cytoplasmic male sterile crop plants contain novel, chimeric open reading frames. In addition, a number of crops carry endogenous double-stranded ribonucleic acid (dsRNA). In this study, the novel proteins encoded by these genetic components were screened for the presence of potentia

  6. Overland flow connectivity in olive orchard plots with cover crops and conventional tillage, and under different rainfall scenarios

    Science.gov (United States)

    López-Vicente, Manuel; García-Ruiz, Roberto; Guzmán, Gema; Vicente-Vicente, José Luis; Gómez, José Alfonso

    2016-04-01

    The study of overland flow connectivity (QC) allows understanding the redistribution dynamics of runoff and soil components as an emergent property of the spatio-temporal interactions of hydrological and geomorphic processes. However, very few studies have dealt with runoff connectivity in olive orchards. In this study we simulated QC in four olive orchard plots, located on the Santa Marta farm (37° 20' 33.6" N, 6° 13' 44" W), in Seville province (Andalusia) in SW Spain. The olive plantation was established in 1985 with trees planted at 8 m x 6 m. Each bounded plot is 8 m wide (between 2 tree lines) and 60 m long (total area of 480 m2), laid out with the longest dimension parallel to the maximum slope and to the tree lines. The slope is uniform, with an average steepness of 11%. Two plots (P2 and P4) were devoted to conventional tillage (CT) consisting of regular chisel plow passes depending on weed growth. Another set of two plots had two types of cover crops (CC) in the inter tree rows (the area outside the vertical olive canopy projection): uniform CC of Lolium multiflorum (P3) and a mixture of L. rigidum and L. multiflorum together with other species (P5). The tree rows were treated with herbicide to keep bare soil. We selected the Index of runoff and sediment Connectivity (IC) of Borselli et al. (2008) to simulate three rainfall scenarios: i) low rainfall intensity (Sc-LowInt) and using the MD flow accumulation algorithm; ii) moderate rainfall intensity (Sc-ModInt) and using MD8; and iii) high rainfall intensity (Sc-HighInt) and using D8. After analysing the values of rainfall intensity during two hydrological years (Oct'09-Sep'10 and Oct'10-Sep'11) we associated the three scenarios with the followings months: Sc-LowInt during the period Jan-Mar, that summarizes 42% of all annual rainfall events; Sc-ModInt during Oct-Nov and Apr-May (32% of all events); and Sc-HighInt during the period Jun-Sep and in December (26% of all events). Instead of using the C

  7. Multi-method comparison of carrot quality from a conventional and three organic cropping systems with increasing levels of nutrient recycling

    DEFF Research Database (Denmark)

    Paoletti, Flavio; Raffo´, Antonio; Kristensen, Hanne Lakkenborg

    2012-01-01

    BACKGROUND: There is a need to advance the study of the effects of organic and conventional systems on product quality. In particular, little is known about the importance of different farming practices concerning nutrient cycling and the use of external inputs within organic farming...... for the quality characteristics of the products. In this study the quality characteristics of carrot grown under different farming practices (conventional and three organic cropping systems) over a two-year period were analysed with the aim of discriminating between organic and conventional and investigating...... the effect of different organic farming practices concerning nutrient recycling and use of external nutrient input. RESULTS: All quality characteristics measured did not give a clear differentiation between the carrots from the different growing systems, even when multivariate statistical evaluation...

  8. Assessment of Dithiocarbamate Residues on Tomatoes Conventionally Grown in Uganda and the Effect of Simple Washing to Reduce Exposure Risk to Consumers

    DEFF Research Database (Denmark)

    Atuhaire, Aggrey; Kaye, Emmanuel; Mutambuze, Innocent Louis

    2017-01-01

    such tomatoes. Results revealed that mancozeb was the only reported dithiocarbamate, and 47.4% and 14% of farm and market samples, respectively, had dithiocarbamate residues exceeding the Codex alimentarius maximum residue limit of 2 mgCS2/kg. Mixing concentration had a positive significant effect......Pesticide misuse by farmers poses health risks to consumers. This study assessed the level of dithiocarbamate residues in tomatoes acquired from 20 farmers and 25 market vendors in Wakiso District, how simple washing affects these residues, and the potential chronic health risk for Ugandans eating...

  9. Host-specific salivary elicitor(s) of European corn borer induce defenses in tomato and maize.

    Science.gov (United States)

    Louis, Joe; Peiffer, Michelle; Ray, Swayamjit; Luthe, Dawn S; Felton, Gary W

    2013-07-01

    Plants turn on induced defenses upon insect herbivory. In the current study, we evaluated the role of European corn borer (ECB) elicitors (molecules secreted by herbivores) that either induce/suppress defenses in Solanum lycopersicum (tomato) and Zea mays (maize), two very important crop plants that are grown for food and/or fuel throughout the world. We used a combination of molecular, biochemical, confocal and scanning electron microscopy, caterpillar spinneret ablation/cauterization, and conventional insect bioassay methods to determine the role of ECB elicitors in modulating defenses in both tomato and maize crop plants. Our results clearly demonstrate that the components present in the ECB saliva induce defense-related proteinase inhibitors in both tomato (PIN2) and maize (MPI). Presence of glucose oxidase in the ECB saliva induced defenses in tomato, but not in maize. However, ECB saliva induced genes present in the jasmonic acid biosynthesis pathway in both tomato and maize. Although ECB saliva can induce defenses in both tomato and maize, our results suggest that host-specific salivary components are responsible for inducing host plant defenses. Proteomic analysis of ECB salivary elicitors and plant receptors/signaling mechanisms involved in recognizing different ECB elicitors remains to be determined. © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

  10. Bioefficacy evaluation and dissipation pattern of nanoformulation versus commercial formulation of pyridalyl in tomato (Solanum lycopersicum).

    Science.gov (United States)

    Saini, Priya; Gopal, Madhuban; Kumar, Rajesh; Gogoi, Robin; Srivastava, Chitra

    2015-08-01

    A study was undertaken to evaluate the decline of the residues of pyridalyl in tomatoes grown in two different cultivation systems: open field (conventional cultivation) and net house (pot experiment). Field experiment was conducted with commercial (10 EC) and nanoformulation of pyridalyl on tomato crop. Tomato plants were subjected to pesticide spray, when fruits were close to ripeness. Fruit samples were taken periodically and cleaned up using QuEChERS methodology, and the residue of pyridalyl was analyzed by ultrahigh-performance liquid chromatography (UHPLC). It dissipated in tomato fruit following the first-order kinetics. In field, average initial deposit of pyridalyl in tomato was observed to be 0.222 and 0.371 μg g(-1) at recommended and double the recommended application rate, respectively, using nanoformulation while it was 0.223 and 0.393 μg g(-1) on using commercial formulation, respectively. The half-life (t1/2) value of nanopyridalyl in tomato fruit was 2.8 and 3.2 days while for commercial formulation, it was 2.5 and 2.6 days for recommended and double the recommended dose, respectively. In India, maximum residue limit (MRL) on tomato has not been fixed for pyridalyl but its residues were always below European MRLs (5 μg g(-1)) on tomato at both application rates. The results of terminal residue showed that pyridalyl residues were below the available MRL. Low residues in tomatoes suggested that this pesticide is safe to use under the recommended dosage. No statistical differences were observed between the cultivation systems in relation to the residue levels of pyridalyl.

  11. Homeopathic and high dilution preparations for pest management to tomato crop under organic production system Manejo fitossanitário do tomateiro com uso de preparados homeopáticos e altas diluições sob sistema orgânico de produção

    Directory of Open Access Journals (Sweden)

    Tatiani A Modolon

    2012-03-01

    Full Text Available Tomato crops (Solanum lycopersicum under conventional production system are constantly treated against pest and diseases, with organic synthetic pesticides that are used may cause serious disturbance to environment and human health. This research was carried out in order to study the effect of homeopathic and high dilution preparations on pests and diseases management of tomato crop under organic production system. Two experiments were conducted under field conditions and one in greenhouse. All of them in randomized block design with the cultivar Santa Cruz Kada. The treatments consisted of homeopathic preparations Staphysagria, Arsenicum album, Sulphur, Arnica montana, high dilution preparations of Solanum lycopersicum and Solanum aculeatissimum, at the decimal (DH or centesimal (CH scale of hahnemanian high dilution. Bordeaux mixture, cupric preparation, and Bacillus thuringiensis were also included in weight doses. The preparation Arnica montana 12DH increased the tomato yield under field conditions. The damage incidence of small borer was efficiently reduced with applications of Sulphur 12CH that equalling to B. thuringiensis. The same treatment did not reduce the large borers. B. thuringiensis reduced also the septoria leaf spot incidence in the field. In greenhouse, the high dilution preparation of tomato at 12DH completely suppressed the septoria leaf spot and drastically reduced the same disease at 24DH. The homeopathic and high dilution preparations have the potential to be effective on replacing pesticides that are used on tomato, and could be suitable to fill legal requirement for organic production system.O tomateiro (Solanum lycopersicum sob sistema de produção convencional está constantemente sujeito a pragas e doenças, os pesticidas orgânicos sintéticos que são usados podem causar graves perturbações ao meio ambiente e à saúde humana. O objetivo deste trabalho foi avaliar o efeito de preparados homeopáticos e altas

  12. Environmental effect of conventional and GM crops of cotton (Gossipium hirsitum L. and corn (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Kelly Ávila

    2011-12-01

    Full Text Available In the corn belt of Valle de San Juan and in the cotton zone of El Espinal, municipalities in the department of Tolima (Colombia, 10 conventional corn producers, 10 producers of genetically modified corn, five producers of conventional cotton and 15 producers of transgenic cotton were surveyed in the first half of 2009 to contrast the differences in the environmental impact associated with use of insecticides and herbicides, which were evaluated by estimating the environmental index quotient-EIQ. In the case of maize, an EIQ of 42 was found in the conventional type, while transgenic technology had an EIQ of 3.03. In the cultivation of cotton, an EIQ of 263.59 was found for the conventional type while for transgenic technology this value varied between 335.75 (Nuopal BG/RR and 324.79 (DP 455 BG/RR. These data showed a lower environmental impact using GM technology in the cultivation of maize when compared to the conventional counterpart, in connection with the use of insecticides and herbicides, in the context of time, space and genotypic analysis. This effect was not observed in the case of cotton, where environmental impacts were similar

  13. Energy efficiency of conventional, organic, and alternative cropping systems for food and fuel at a site in the U.S. Midwest.

    Science.gov (United States)

    Gelfand, Ilya; Snapp, Sieglinde S; Robertson, G Philip

    2010-05-15

    The prospect of biofuel production on a large scale has focused attention on energy efficiencies associated with different agricultural systems and production goals. We used 17 years of detailed data on agricultural practices and yields to calculate an energy balance for different cropping systems under both food and fuel scenarios. We compared four grain and one forage systems in the U.S. Midwest: corn (Zea mays) - soybean (Glycine max) - wheat (Triticum aestivum) rotations managed with (1) conventional tillage, (2) no till, (3) low chemical input, and (4) biologically based (organic) practices, and (5) continuous alfalfa (Medicago sativa). We compared energy balances under two scenarios: all harvestable biomass used for food versus all harvestable biomass used for biofuel production. Among the annual grain crops, average energy costs of farming for the different systems ranged from 4.8 GJ ha(-1) y(-1) for the organic system to 7.1 GJ ha(-1) y(-1) for the conventional; the no-till system was also low at 4.9 GJ ha(-1) y(-1) and the low-chemical input system intermediate (5.2 GJ ha(-1) y(-1)). For each system, the average energy output for food was always greater than that for fuel. Overall energy efficiencies ranged from output:input ratios of 10 to 16 for conventional and no-till food production and from 7 to 11 for conventional and no-till fuel production, respectively. Alfalfa for fuel production had an efficiency similar to that of no-till grain production for fuel. Our analysis points to a more energetically efficient use of cropland for food than for fuel production and large differences in efficiencies attributable to management, which suggests multiple opportunities for improvement.

  14. Toxicidade de inseticidas utilizados na cultura do tomateiro a Trichogramma pretiosum Toxicity of insecticides used in tomato crop to Trichogramma pretiosum

    Directory of Open Access Journals (Sweden)

    Alexandre Pinho Moura

    2005-03-01

    .4%, respectively, the emergence of individuals of the F1 generation of T. pretiosum. No insecticide affected the sex ratio of the F1 generation of this parasitoid. Chlorfenapyr reduced in approximately 50% the parasitization rate of individuals of the F1 generation. Both acetamiprid and thiamethoxam were harmless to T. pretiosum, so that they can be used for the control of pests in tomato crop with this parasitoid.

  15. Impacts of organic and conventional crop management on diversity and activity of free-living nitrogen fixing bacteria and total bacteria are subsidiary to temporal effects.

    Science.gov (United States)

    Orr, Caroline H; Leifert, Carlo; Cummings, Stephen P; Cooper, Julia M

    2012-01-01

    A three year field study (2007-2009) of the diversity and numbers of the total and metabolically active free-living diazotophic bacteria and total bacterial communities in organic and conventionally managed agricultural soil was conducted using the Nafferton Factorial Systems Comparison (NFSC) study, in northeast England. Fertility management appeared to have little impact on both diazotrophic and total bacterial communities. However, copy numbers of the nifH gene did appear to be negatively impacted by conventional crop protection measures across all years suggesting diazotrophs may be particularly sensitive to pesticides. Impacts of crop management were greatly overshadowed by the influence of temporal effects with diazotrophic communities changing on a year by year basis and from season to season. Quantitative analyses using qPCR of each community indicated that metabolically active diazotrophs were highest in year 1 but the population significantly declined in year 2 before recovering somewhat in the final year. The total bacterial population in contrast increased significantly each year. It appeared that the dominant drivers of qualitative and quantitative changes in both communities were annual and seasonal effects. Moreover, regression analyses showed activity of both communities was significantly affected by soil temperature and climatic conditions.

  16. Brazilian Free-tailed Bats (Tadarida brasiliensis) as Insect Pest Regulators in Transgenic and Conventional Cotton Crops

    Science.gov (United States)

    During the past 12,000 years agricultural systems have transitioned from natural habitats to conventional agricultural regions, and recently to large areas of genetically- engineered (GE) croplands. This GE revolution occurred for cotton in a span of slightly more than a decade w...

  17. Environmental effect of conventional and GM crops of cotton (Gossypium hirsutum L. and corn (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Chaparro Giraldo Alejandro

    2011-12-01

    Full Text Available

    In the corn belt of Valle de San Juan and in the cotton zone of El Espinal, municipalities in the department of Tolima (Colombia, 10 conventional corn producers, 10 producers of genetically modified corn, five producers of conventional cotton and 15 producers of transgenic cotton were surveyed in the first half of 2009 to contrast the differences in the environmental impact associated with use of insecticides and herbicides, which were evaluated by estimating the environmental index quotient-EIQ. In the case of maize, an EIQ of 42 was found in the conventional type, while transgenic technology had an EIQ of 3.03. In the cultivation of cotton, an EIQ of 263.59 was found for the conventional type while for transgenic technology this value varied between 335.75 (Nuopal BG/RR and 324.79 (DP 455 BG/RR. These data showed a lower environmental impact using GM technology in the cultivation of maize when compared to the conventional counterpart, in connection with the use of insecticides and herbicides, in the context of time, space and genotypic analysis. This effect was not observed in the case of cotton, where environmental impacts were similar.

  18. Analysis of Differences in Productivity, Proiftability and Soil Fertility Between Organic and Conventional Cropping Systems in the Tropics and Sub-tropics

    Institute of Scientific and Technical Information of China (English)

    Te Pas CM; Rees RM

    2014-01-01

    Organic farming aims to stimulate soil fertility by avoiding the use of synthetic fertiliser inputs, relying instead on locally available natural resources. It is regarded by many as a sustainable alternative to conventional farming because it ensures higher biodiversity, restricts environmental pollution, prevents land degradation and is easy to apply for smallholder and subsistence farmers. Although widely practiced and studied in temperate regions, little is known about the potential overall beneifts of organic farming in the tropics and subtropics. This paper addresses this gap by undertaking an analysis of the differences between organic and conventional agriculture in the tropics and sub-tropics based on an extensive literature review including 88 papers with 458 data pairs. The comparison is based on three main indicators:yield, gross margin and soil organic carbon (SOC). The differences between the organic and conventional systems for each of these main indicators is represented by the ratio of the value of the indicator in the organic system divided by the corresponding value in the conventional system. This was initially calculated for each data pair individually, and grouped by a variety of explanatory factors, such as precipitation, human development level, soil texture, crop type, organic input type, time after conversion and certiifcation. The results demonstrate that under organic management, yields were on average 26%higher, gross margins 51%higher and soil organic carbon 53%higher than under conventional management. The highest yield increases in organic cropping systems were achieved in the least developed countries, in arid regions and on coarse soils. For gross margins, certiifcation was the main reason for differences between organic and conventional systems. Certiifed farmers, mostly located in developed countries, receive signiifcantly higher prices. Furthermore, organic farming in the driest regions results in higher proifts than in other

  19. The application of high-concentration short-time chlorine dioxide treatment for selected specialty crops including Roma tomatoes (Lycopersicon esculentum), cantaloupes (Cucumis melo ssp. melo var. cantaloupensis) and strawberries (Fragaria×ananassa).

    Science.gov (United States)

    Trinetta, V; Linton, R H; Morgan, M T

    2013-06-01

    The effects of high-concentration short-time chlorine dioxide (ClO2) gas treatment on food-borne pathogens inoculated onto the surface of tomatoes, cantaloupes, and strawberries were studied. Produce were spot-inoculated with a mixture of Salmonella enterica (serotypes Montevideo, Javiana and Baildon), Escherichia coli O157:H7 (serotypes 204 P, EDL 933 and C792) or Listeria monocytogenes (serotypes Scott A, F 5069 and LCDC 81-861), and treated with ClO2 gas at 10 mg/l for 180 s. After ClO2 gas treatment, surviving populations were determined and shelf-life studies were conducted (microbial spoilage population, change in color and overall appearance). Significant microbial reduction (p 0.05). Results obtained suggest the potential use of high-concentration short-time ClO2 gas treatment as an effective online pathogen inactivation technology for specialty crops in large-scale produce packing operations.

  20. The use of crop life tables as a tomato yield loss management tool = Uso de tabelas de vida das culturas como ferramenta de manejo de perdas de produtividade no tomateiro

    Directory of Open Access Journals (Sweden)

    Rodolf Araújo Loos

    2007-10-01

    Full Text Available The objective of this work was the identification and quantification of tomato yield loss components in the field and in greenhouse, evidencing the tomato critical production component and the loss key-factor, using the crop life table methodology. Two experiments (field and greenhouse consisting of two treatments (variety Santa Clara and hybrid Débora Plus and five replications were conducted in Viçosa, state of Minas Gerais, Brazil. During the tomato plant cycle, the number of dead plants and the death causes were evaluated and the number of flowers and fruits/plant was recorded. During harvesting, the healthy and damaged fruits were counted, weighed and classified and the causes of loss of the damaged fruits were identified. In the field, plants were considered the critical production component of tomato yield. The TSWV virus was considered the loss keyfactor.In the greenhouse, fruits were considered the critical production component. Blossom-end rot was considered the loss key-factor.O objetivo deste trabalho foi a identificação e quantificação dos componentes de perdas de produtividade do tomateiro no campo eambiente protegido, evidenciando o componente crítico e o fator-chave de perdas de produtividade da cultura, utilizando a metodologia tabela de vida das culturas. Dois experimentos (campo e ambiente protegido, consistindo de dois tratamentos (variedade Santa Clara e híbrido Débora Plus e cinco repetições, foram conduzidos em Viçosa, Estado de Minas Gerais, Brasil. Durante o ciclo do tomateiro, o número de plantas mortas, causas de morte e o número de flores e frutos/planta foram avaliados. Na colheita, os frutos comerciais e danificados foram contados, pesados e classificados e as causas de perda foram identificadas. No campo, o componente de produção plantas foi considerado o componente crítico de perdas. O vírus TSWV foi considerado o fator-chave de perdas. Em ambiente protegido, frutos foi considerado o componente

  1. Within-plant distribution and sampling of single and mixed infestations of Bemisia tabaci and Trialeurodes vaporariorum (Homoptera: Aleyrodidae) in winter tomato crops.

    Science.gov (United States)

    Arnó, Judit; Albajes, Ramon; Gabarra, Rosa

    2006-04-01

    In several areas of Spain, the greenhouse whitefly, Trialeurodes vaporariorum (Westwood), and the sweet potato whitefly, Bemisia tabaci (Gennadius), coexist in tomato, Lycopersicon esculentum Miller. For integrated pest management decision-making, it is important to know the abundance of each species, because they exhibit different abilities to transmit viruses, are susceptible to different biological control agents, and have different responses to insecticides. This study was conducted to provide information on the vertical distribution of T. vaporariorumn and B. tabaci in tomato plants grown in greenhouses in winter and to determine the optimal sampling unit and the sample size for estimating egg and nymphal densities of both whitefly species. Eggs of T. vaporariorum were mainly located on the top stratum of the plant, whereas B. tabaci eggs were mainly found on the middle stratum. Nymphs of both species mainly concentrated in the bottom stratum of the plant. When pest abundance and low relative variation were considered, the bottom stratum was selected as the most convenient for sampling nymphs of both whitefly species. Conversely, the same two criteria indicated that either the top or the middle strata could be used when sampling T. vaporariorum and B. tabaci eggs. Several different sampling units were compared to optimize the estimation of nymphal and egg densities in terms of cost efficiency. One disk (1.15 cm in diameter) per leaflet collected from the top stratum of the tomato plant was the most efficient sampling unit for simultaneously estimating the egg densities of the two whitefly species.

  2. Effects of organic and conventional management of sugar cane crop on soil physicochemical characteristics and phosphomonoesterase activity.

    Science.gov (United States)

    Purcena, Luiza L A; Di Medeiros, Maria Carolina B; Leandro, Wilson M; Fernandes, Kátia F

    2014-02-19

    Soil enzymes play an important role in agriculture and particularly in nutrient cycling. They are also involved in the degradation, transformation, and mineralization of organic matter and availability of nutrients in soil. It is believed that organic agriculture causes fewer losses to soil quality and is less aggressive to the environment than conventional management. In this study, the effects of conventional (CM) and organic management (OM) on phosphomonoesterases, an important enzyme for soil fertility, were evaluated and compared to those results from native Cerrado (Brazilian Savanna) soil (NS), because they are the most common phosphatases in soils. The results showed that there were both acid (AcP) and alkaline (AkP) phosphatases in all soils tested and that AcP activity was higher than that of AkP. In contrast to AkP, AcP had its activity affected by land use. In the cultivated areas there was a reduction of almost 50% of AcP activity respect to native unexploited soils and there was no significant difference between organic and CM, demonstrating that independent of the management chosen, there was an impact of land use on AcP activity. Principal component analysis indicated that characteristics related to pH such as alkali saturation (V%), aluminum saturation (M%), Al(3+), soil total acidity (H+Al), and Ca(2+) are the main factors that permit distinguishing NS from OM and CM.

  3. Effect of crop protection and fertilization regimes used in organic and conventional production systems on feed composition and physiological parameters in rats.

    Science.gov (United States)

    Srednicka-Tober, Dominika; Barański, Marcin; Gromadzka-Ostrowska, Joanna; Skwarło-Sońta, Krystyna; Rembiałkowska, Ewa; Hajslova, Jana; Schulzova, Vera; Cakmak, Ismail; Öztürk, Levent; Królikowski, Tomasz; Wiśniewska, Katarzyna; Hallmann, Ewelina; Baca, Elżbieta; Eyre, Mick; Steinshamn, Håvard; Jordon, Teresa; Leifert, Carlo

    2013-02-01

    Very little is known about the effects of an organic or conventional diet on animal physiology and health. Here, we report the effect of contrasting crop protection (with or without chemosynthetic pesticides) and fertilization (manure or mineral fertilizers) regimes on feed composition and growth and the physiological parameters of rats. The use of manure instead of mineral fertilizers in feed production resulted in lower concentrations of protein (18.8 vs 20.6%) and cadmium (3.33 vs 4.92 μg/100 g) but higher concentrations of polyphenols (1.46 vs 0.89 g/100 g) in feeds and higher body protein (22.0 vs 21.5%), body ash (3.59 vs 3.51%), white blood cell count (10.86 vs 8.19 × 10³/mm³), plasma glucose (7.23 vs 6.22 mmol/L), leptin (3.56 vs 2.78 ng/mL), insulin-like growth factor 1 (1.87 vs 1.28 μg/mL), corticosterone (247 vs 209 ng/mL), and spontaneous lymphocyte proliferation (11.14 vs 5.03 × 10³ cpm) but lower plasma testosterone (1.07 vs 1.97 ng/mL) and mitogen stimulated proliferation of lymphocytes (182 vs 278 × 10³ cpm) in rats. There were no main effects of crop protection, but a range of significant interactions between fertilization and crop protection occurred.

  4. Quantitative proteomics by 2DE and MALDI MS/MS uncover the effects of organic and conventional cropping methods on vegetable products

    DEFF Research Database (Denmark)

    Nawrocki, Arkadiusz; Thorup-Kristensen, Kristian; Jensen, Ole Nørregaard

    2011-01-01

    of slurry, in accordance to regulations of organic farming and O2, in which nutrient supply was based mainly on autumn green manures. Proteins were extracted from lyophilized plant tissues into a buffer containing high concentrations of urea/thiourea, two detergents and reducing agent. This approach allowed...... short handling times of fresh plant materials. In the case of cabbage samples, the abundance levels of 58 out of more than 1300 quantified protein spots varied significantly between conventional farming and any of the organic cropping systems. Proteome profiles were also very similar between carrot root...... samples, where 68 out of 1800 resolved protein spots varied significantly. Proteins of the glycolytic pathway and Krebs cycle as well as several proteins related to amino acid and protein metabolism were overexpressed in organically farmed cabbage. Proteins related to detoxification processes were...

  5. Monoculture Maize (Zea mays L. Cropped Under Conventional Tillage, No-tillage and N Fertilization: (II Fumonisin Incidence on Kernels

    Directory of Open Access Journals (Sweden)

    Carolina Gavazzi

    2009-09-01

    Full Text Available Planting maize under no-tillage is an increasing farming practice for sustainable agriculture and sound environmental management. Although several studies on yield of no-till maize have been done, there is few information about the effect of tillage on fumonisin contamination. The present study was done to determine the effect of notillage and conventional tillage with two rates of nitrogen on fumonisin content in kernels of continuous maize. Average grain contamination with fumonisins B1 and B2 over the years 2004-06 was not significantly different, with mean values of 1682, 1984 and 2504 μg kg-1, respectively. Fumonisin B1 was the most abundant toxin found in the samples. No-tillage significantly affected the incidence of fumonisins during the first year of the trial, in which fumonisin content was significantly higher with no-till (2008 μg kg-1 compared with conventional tillage (1355 μg kg-1. However, no-tillage did not significantly affect the incidence of fumonisins in the second and third years of the study. Fumonisin content at the rate of 300 kg N ha-1 was not statistically different compared to that obtained without N fertilization. The interaction between the soil management system and the rate of applied nitrogen was only evident in the second year. Our results indicate that fumonisin contamination was affected by no-tillage only in the first year. Nitrogen fertilization had no significant effect on fumonisin content in any year. The weather conditions during susceptible stages of maize development have probably overridden the effect of nitrogen fertilization.

  6. Tomato Preserves.

    Science.gov (United States)

    Stevens, Wendy Tessman

    1996-01-01

    Describes a project in which students selected seeds from two heirloom varieties of tomatoes, sowed the seeds, harvested the tomatoes, and fermented the seeds. Details are provided for each step of the project and the school address is included so that other students can begin similar projects. (DDR)

  7. THE CROP YIELD GAP BETWEEN ORGANIC AND CONVENTIONAL AGRICULTURE%有机—常规农业中作物产量差距分析

    Institute of Scientific and Technical Information of China (English)

    托曼克·德·波帝; 波特·里克; 马丁·克·范·伊特松

    2015-01-01

    to feed the world. Comparisons of organic and conventional yields play a central role in this debate. We therefore compiled and analyzed a meta-dataset of 362 published organ-ic-conventional comparative crop yields. Our results show that organic yields of individual crops are on average 80%of conventional yields,but variation is substantial( standard deviation 21%) . In our dataset,the organic yield gap significantly differed between crop groups and regions. The analysis gave some support to our hypothesis that the or-ganic-conventional yield gap increases as conventional yields increase, but this relationship was only rather weak. The rationale behind this hypothesis is that when conventional yields are high and relatively close to the po-tential or water-limited level,nutrient stress must,as per definition of the potential or water-limited yield levels,be low and pests and diseases well controlled,which are conditions more difficult to attain in organic agriculture. We discuss our findings in the context of the literature on this subject and address the issue of upscaling our results to higher system levels. Our analysis was at field and crop level. We hypothesize that due to challenges in the mainte-nance of nutrient availability in organic systems at crop rotation,farm and regional level,the average yield gap be-tween conventional and organic systems may be larger than 20% at higher system levels. This relates in particular to the role of legumes in the rotation and the farming system,and to the availability of( organic) manure at the farm and regional levels. Future research should therefore focus on assessing the relative performance of both types of agricul-ture at higher system levels,i. e. the farm,regional and global system levels,and should in that context pay particu-lar attention to nutrient availability in both organic and conventional agriculture.

  8. Assessment of region, farming system, irrigation source and sampling time as food safety risk factors for tomatoes.

    Science.gov (United States)

    Pagadala, Sivaranjani; Marine, Sasha C; Micallef, Shirley A; Wang, Fei; Pahl, Donna M; Melendez, Meredith V; Kline, Wesley L; Oni, Ruth A; Walsh, Christopher S; Everts, Kathryne L; Buchanan, Robert L

    2015-03-02

    In the mid-Atlantic region of the United States, small- and medium-sized farmers use varied farm management methods and water sources to produce tomatoes. It is unclear whether these practices affect the food safety risk for tomatoes. This study was conducted to determine the prevalence, and assess risk factors for Salmonella enterica, Shiga toxin-producing Escherichia coli (STEC) and bacterial indicators in pre-harvest tomatoes and their production areas. A total of 24 organic and conventional, small- to medium-sized farms were sampled for six weeks in Maryland (MD), Delaware (DE) and New Jersey (NJ) between July and September 2012, and analyzed for indicator bacteria, Salmonella and STEC. A total of 422 samples--tomato fruit, irrigation water, compost, field soil and pond sediment samples--were collected, 259 of which were tomato samples. A low level of Salmonella-specific invA and Shiga toxin genes (stx1 or stx2) were detected, but no Salmonella or STEC isolates were recovered. Of the 422 samples analyzed, 9.5% were positive for generic E. coli, found in 5.4% (n=259) of tomato fruits, 22.5% (n=102) of irrigation water, 8.9% (n=45) of soil, 3/9 of pond sediment and 0/7 of compost samples. For tomato fruit, farming system (organic versus conventional) was not a significant factor for levels of indicator bacteria. However, the total number of organic tomato samples positive for generic E. coli (1.6%; 2/129) was significantly lower than for conventional tomatoes (6.9% (9/130); (χ(2) (1)=4.60, p=0.032)). Region was a significant factor for levels of Total Coliforms (TC) (p=0.046), although differences were marginal, with western MD having the highest TC counts (2.6 log CFU/g) and NJ having the lowest (2.0 log CFU/g). Tomatoes touching the ground or plastic mulch harbored significantly higher levels of TC compared to vine tomatoes, signaling a potential risk factor. Source of irrigation water was a significant factor for all indicator bacteria (p<0.0001), and

  9. Incidência de pragas e desempenho produtivo de tomateiro orgânico em monocultivo e policultivo Incidence of pests and organic tomato crop productive performance in monoculture and polyculture

    Directory of Open Access Journals (Sweden)

    Flávia B Gomes

    2012-12-01

    Full Text Available O objetivo com este experimento foi comparar o cultivo do tomateiro em monocultivo e policultivo (consórcio com coentro, cravo-de-defunto e o sorgo como cultura circundante no sistema de produção orgânico, em relação à incidência de pragas, rendimento e qualidade de frutos. Foram avaliados a incidência de tripes, plantas e frutos com sintomas de virose (%, frutos broqueados por Neoleucinodes elegantalis (%, a produtividade total e comercial, o número total e comercial de frutos, o peso médio e o diâmetro dos frutos comercializáveis, o teor de sólidos solúveis totais (SST, o teor de acidez total titulável (ATT e a relação SST/ATT. No policultivo ocorreu menor número de tripes e, consequentemente, menor percentagem de plantas e frutos com sintomas da virose. A incidência de broqueamento de frutos por N. elegantalis também foi menor em policultivo. A produtividade total e o número total de frutos no monocultivo foram superiores à do policultivo. Porém, a produtividade comercial foi igual nos dois sistemas de cultivo. O diâmetro dos frutos não diferiu entre os tratamentos, mas o peso médio dos frutos oriundos do monocultivo foi maior. Os frutos do policultivo apresentaram maiores teores de SST e ATT e a relação SST/ATT não diferiu entre os tratamentos. Assim, reforça-se a importância da diversidade vegetal no manejo de pragas, sendo necessários novos estudos que permitam definir melhor arranjo espacial das plantas para manter sua atuação na regulação de insetos-praga e qualidade dos frutos sem interferir na produtividade do tomateiro.The tomato crop in monoculture and polyculture (intercropping with coriander and marigold and sorghum as surrounding crop were compared in organic system of production, in relation to incidence of pests, yield and quality of fruits. We evaluated the incidence of thrips, the plants and fruits with symptoms of viral disease (%, damaged fruits by Neoleucinodes elegantalis (%, total and

  10. Transcriptome profiling to discover defense-related genes associated with resistance line ty-5 against Tomato yellow leaf curl virus in tomato

    Science.gov (United States)

    Tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted begomovirus, has caused serious economic losses to tomato crops in the U.S. and around the world. The most effective management would be the use of a TYLCV-resistant tomato cultivar. Several sources of TYLCV resistance genes have been ide...

  11. A decision support system (GesCoN) for managing fertigation in vegetable crops. Part II—model calibration and validation under different environmental growing conditions on field grown tomato

    Science.gov (United States)

    Conversa, Giulia; Bonasia, Anna; Di Gioia, Francesco; Elia, Antonio

    2015-01-01

    The GesCoN model was evaluated for its capability to simulate growth, nitrogen uptake, and productivity of open field tomato grown under different environmental and cultural conditions. Five datasets collected from experimental trials carried out in Foggia (IT) were used for calibration and 13 datasets collected from trials conducted in Foggia, Perugia (IT), and Florida (USA) were used for validation. The goodness of fitting was performed by comparing the observed and simulated shoot dry weight (SDW) and N crop uptake during crop seasons, total dry weight (TDW), N uptake and fresh yield (TFY). In SDW model calibration, the relative RMSE values fell within the good 10–15% range, percent BIAS (PBIAS) ranged between −11.5 and 7.4%. The Nash-Sutcliffe efficiency (NSE) was very close to the optimal value 1. In the N uptake calibration RRMSE and PBIAS were very low (7%, and −1.78, respectively) and NSE close to 1. The validation of SDW (RRMSE = 16.7%; NSE = 0.96) and N uptake (RRMSE = 16.8%; NSE = 0.96) showed the good accuracy of GesCoN. A model under- or overestimation of the SDW and N uptake occurred when higher or a lower N rates and/or a more or less efficient system were used compared to the calibration trial. The in-season adjustment, using the “SDWcheck” procedure, greatly improved model simulations both in the calibration and in the validation phases. The TFY prediction was quite good except in Florida, where a large overestimation (+16%) was linked to a different harvest index (0.53) compared to the cultivars used for model calibration and validation in Italian areas. The soil water content at the 10–30 cm depth appears to be well-simulated by the software, and the GesCoN proved to be able to adaptively control potential yield and DW accumulation under limited N soil availability scenarios and consequently to modify fertilizer application. The DSSwell simulate SDW accumulation and N uptake of different tomato genotypes grown under Mediterranean and

  12. A Decision Support System (GesCoN for Managing Fertigation in Vegetable Crops. Part II – Model calibration and validation under different environmental growing conditions on field grown tomato

    Directory of Open Access Journals (Sweden)

    Giulia eConversa

    2015-07-01

    Full Text Available The GesCoN model was evaluated for its capability to simulate growth, nitrogen uptake and productivity of open field tomato grown under different environmental and cultural conditions. Five datasets collected from experimental trials carried out in Foggia (IT were used for calibration and 13 datasets collected from trials conducted in Foggia, Perugia (IT and Florida (USA were used for validation. The goodness of fitting was performed by comparing the observed and simulated shoot dry weight (SDW and N crop uptake during crop seasons, total dry weight (TDW, N uptake and fresh yield (TFY. In SDW model calibration, the relative RMSE values fell within the good 10 to 15% range, percent BIAS (PBIAS ranged between -11.5% and 7.4%. The Nash-Sutcliffe efficiency (NSE was very close to the optimal value 1. In the N uptake calibration RRMSE and PBIAS were very low(7%, and -1.78, respectively and NSE close to 1. The validation of SDW (RRMSE=16.7%; NSE=0.96 and N uptake (RRMSE=16.8%; NSE=0.96 showed the good accuracy of GesCoN. A model under- or overestimation of the SDW and N uptake occurred when higher or a lower N rates and/or a more or less efficient system were used compared to the calibration trial. The in-season adjustment, using the SDWcheck procedure, greatly improved model simulations both in the calibration and in the validation phases. The TFY prediction was quite good except in Florida, where a large overestimation (+16% was linked to a different harvest index (0.53 compared the cultivars used for model calibration and validation in Italian areas. The soil water content at the 10-30 cm depth appears to be well simulated by the software, and the GesCoN proved to be able to adaptively control potential yield and DW accumulation under limited N soil availability scenarios and consequently to modify fertilizer application. The DSSwell simulate SDW accumulation and N uptake of different tomato genotypes grown under Mediterranean and subtropical

  13. Effects of Continuous Cropping of Processing Tomato on Physical-chemical Properties of and Microbial Biomass in the Soil%加工番茄连作对土壤理化性状及微生物量的影响

    Institute of Scientific and Technical Information of China (English)

    康亚龙; 景峰; 孙文庆; 谈建鑫; 冉辉; 蒋桂英

    2016-01-01

    通过在石河子大学农学院试验站开展加工番茄连作定点微区试验,研究了不同连作处理(种植1 a、连作3 a、5 a和7 a)对新疆加工番茄土壤理化性状、微生物生物量和酶活性的影响。结果表明,随着连作年限的延长,土壤pH升高,全磷、速效磷及全钾含量呈先升后降的趋势,土壤容重无明显变化。连作7 a时土壤有机质、全氮及速效钾含量较对照分别下降了8%、21%和29%(p<0.05)。土壤微生物量碳(SMBC)、微生物量氮(SMBN)和微生物商(qMB)呈显著下降趋势,与对照相比分别降低了52.3%、78.8%和48.2%(p<0.01);微生物量磷(SMBP)呈先升后降趋势,连作3a时,SMBP含量达到最大值,是对照的1.65倍(p<0.01)。土壤过氧化氢酶活性呈显著升高趋势,而脲酶、蔗糖酶、多酚氧化酶及磷酸酶活性的变化则相反。连作导致加工番茄产量显著下降,连作7 a时产量下降达34%(p<0.01)。相关分析表明,pH、微生物量、qMB、酶活性及养分之间相关性极为密切,说明土壤微生物量和酶活性相结合,可以反映土壤质量的变化。加工番茄连作导致土壤pH和电导率升高,显著抑制了土壤微生物活性,降低了土壤肥力,最终造成产量下降,连作障碍明显。%The Xinjiang Uyghur Autonomous Region is the main processing tomato production area of China. So production of processing tomatoes is the backbone of the local economy. To meet the demand for processing tomatoes of the market,continuous cropping or monocropping is widely adopted for the production of processing tomatoes,but unfortunately it has become the main factor limiting stable production and high yield of the crop in the region. It is,therefore,important to understand the mechanism of continuous tomato cropping regulating soil microbial activity. The objective of this study was to explore effects of continuous tomato

  14. Water use and onion crop production in no-tillage and conventional cropping systems Uso de água e produção de cebola em sistemas de plantio direto e convencional

    Directory of Open Access Journals (Sweden)

    Waldir Aparecido Marouelli

    2010-03-01

    Full Text Available The objective of the present study was to evaluate the effects of crop residue covers (0.0; 4.5; 9.0; 13.5 t ha-1 millet dry matter on water use and production of onion cultivated in no-tillage planting system (NT as compared to conventional tillage system (CT. The study was carried out at Embrapa Hortaliças, Brazil, under the typical Savanna biome. Irrigations were performed using a sprinkle irrigation system when soil-water tension reached between 25 and 30 kPa. The experimental design was randomized blocks with three replications. Total net water depth applied to NT treatment was 19% smaller than the CT treatment, however, water savings increased to 30% for the first 30 days following seedlings transplant. Crop biomass, bulb size and yield, and rate of rotten bulbs were not significantly affected by treatments. The water productivity index increased linearly with increasing crop residue in NT conditions. Water productivity index of NT treatments with crop residue was on average 30% higher than that in the CT system (8.13 kg m-3.O objetivo do presente estudo foi avaliar o efeito do nível de palhada no solo (0,0; 4,5; 9,0; 13,5 t ha-1 de matéria seca de milheto em sistema de plantio direto (PD sobre o uso de água e produção de cebola, tendo como controle o sistema de plantio convencional (PC. O ensaio foi conduzido na Embrapa Hortaliças, em região típica do bioma Cerrado. As irrigações foram realizadas por aspersão a todo o momento que a tensão de água no solo atingiu entre 25 e 30 kPa. O delineamento experimental foi blocos ao acaso com três repetições. A lâmina de água aplicada em PD foi de até 19% menor que no tratamento PC durante o ciclo da cultura, sendo que durante os primeiros 30 dias do ciclo após o transplante das mudas a economia chegou a 30%. O desenvolvimento de plantas, o tamanho e o rendimento de bulbos, e a taxa de bulbos podres não foram afetados significativamente pelos tratamentos. O índice de

  15. Water pressure head and temperature impact on isoxaflutole degradation in crop residues and loamy surface soil under conventional and conservation tillage management.

    Science.gov (United States)

    Alletto, Lionel; Coquet, Yves; Bergheaud, Valérie; Benoit, Pierre

    2012-08-01

    Laboratory incubations were performed in order to evaluate the dissipation of the proherbicide isoxaflutole in seedbed layer soil samples from conventional and conservation tillage systems and in maize and oat residues left at the soil surface under conservation tillage. The effects of temperature and water pressure head on radiolabelled isoxaflutole degradation were studied for each sample for 21d. Mineralisation of isoxaflutole was low for all samples and ranged from 0.0% to 0.9% of applied (14)C in soil samples and from 0.0% to 2.4% of applied (14)C in residue samples. In soil samples, degradation half-life of isoxaflutole ranged from 9 to 26h, with significantly higher values under conservation tillage. In residue samples, degradation half-life ranged from 3 to 31h, with significantly higher values in maize residues, despite a higher mineralisation and bound residue formation than in oat residues. Whatever the sample, most of the applied (14)C remained extractable during the experiment and, after 21d, less than 15% of applied (14)C were unextractable. This extractable fraction was composed of diketonitrile, benzoic acid derivative and several unidentified metabolites, with one of them accounting for more than 17% of applied (14)C. This study showed that tillage system design, including crop residues management, could help reducing the environmental impacts of isoxaflutole.

  16. Towards the development of integrated cultural control of tomato late blight (Phytophthora infestans) in Uganda

    NARCIS (Netherlands)

    Tumwine, J.

    1999-01-01

    Tomato ( Lycopersicon esculentum ) is a major vegetable crop in Uganda. Moneymaker, Marglobe, Heinz and Roma are the major commercial varieties grown in the country, the first two being the most popular. Late blight ( Phytophthora infestans ) is the most important disease of Ugandan tomatoes. Tomato

  17. Partial stem and leaf resistance against the fungal pathogen Botrytis cinerea in wild relatives of tomato

    NARCIS (Netherlands)

    Have, ten A.; Berloo, van R.; Lindhout, P.; Kan, van J.A.L.

    2007-01-01

    Tomato (Solanum lycopersicum) is one of many greenhouse crops that can be infected by the necrotrophic ascomycete Botrytis cinerea. Commercial cultivation of tomato is hampered by the lack of resistance. Quantitative resistance has been reported in wild tomato relatives, mostly based on leaf assays.

  18. Produtividade biológica de genótipos de tomateiro em sistema hidropônico no outono/inverno Performance of tomato genotypes in hydroponic system and fall/winter crop-season

    Directory of Open Access Journals (Sweden)

    Antonio AR de Albuquerque Neto

    2012-12-01

    their biological productivity in hydroponics and fall/winter crop-season, characterized by low solar radiation availability, focusing vegetative and productive aspects, fotoassimilates partitioning and photosynthetic active radiation (PAR use efficiency in order to determine the most promising for greenhouse cultivation in southern Rio Grande do Sul. The experimental design was randomized blocks with 7 treatments (genotypes and 3 replications with 6 plants per plot. Among the evaluated genotypes, five are minitomatoes: Cereja Pendente Yubi Feltrin®(determinated growth habit, Cereja Vermelho ISLA®, Minitomate Pêra Amarelo TOP SEED®, Grape (no commercial, Flavor Top (no commercial. Also, Santa Cruz Kada Gigante TOP SEED® and Gaúcho TOP SEED® tomato varieties were evaluated. Fresh and dry matter productivity (g m-2 of leaves, stems and fruits; leaf area; plant height and compactness; incident global and PAR solar radiations were evaluated. The genotypes Kada Gigante, of Santa Cruz group, and Cereja Pendente Yubi Feltrin®, of determinated growth habit and minitomato group, presented higher fruit yield and PAR use efficiency. So, these genotypes can be considered the most promising for growing in low solar radiation availability crop-season.

  19. Tomato yield and soil chemical attributes depending on previous cover crops Produtividade do tomateiro e atributos químicos do solo em função do uso de plantas de cobertura antecedendo o cultivo

    Directory of Open Access Journals (Sweden)

    Alexandre G Galvão

    2013-03-01

    Full Text Available The influence of different cover crops was evaluated over the agronomic performance of tomato hybrids for industrial processing, as well as its effect over soil chemical attributes. The experimental design was completely randomized, in a split plot scheme, with four replications. Main treatments (plots were composed of winter cover crops (oat, hairy vetch, clover and radish and of a fallow area (spontaneous vegetation. Subplots were composed of four processing tomato hybrids (AP529, AP533, Kátia and Sicílio. We evaluated the total production (TP, marketable production (CP, average mass of marketable fruits (AMCF and number of marketable fruits (NCF. Chemical analysis of soil was done in two stages: one week preceding implantation of cover crops and in the phase of tomato implantation. Highest TPs were obtained in treatments in which cover crops were composed by hairy vetch and radish. However, although hairy vetch has caused an increase in TP, no difference between covers was obtained in relation to CP. Sicílio hybrid presented the greatest AMCF, however, its TP was lower than expected. This fact is related to lower NCF, which was half of the observed in AP529 and AP533 hybrids. Radish cover increased phosphorus, calcium and potassium in soil and this could be one of the factors responsible for the increase of TP provided by radish. On the other hand, oat has caused inverse effect, reducing availability of Ca and K. All covers have increased organic matter in soil, the major increment being presented by oat. Based on these results we conclude that hairy vetch and radish are the most indicated plants for cover preceding tomato cultivation.Foram avaliadas diferentes coberturas de solo sobre o desempenho agronômico de híbridos de tomate para processamento industrial, bem como seus efeitos sobre atributos químicos do solo. O delineamento experimental foi em blocos casualizados, com quatro repetições, no esquema de parcelas subdivididas. Os

  20. Rendimiento de plantas de tomate injertadas y efecto de la densidad de tallos en el sistema hidropónico Yield of tomato crop as a result of grafting and shoot density in hydroponic system

    Directory of Open Access Journals (Sweden)

    Roberta M.N. Peil

    2004-06-01

    superficie; asimismo, dio un tamaño más uniforme de fruto, comparando con plantas cultivadas desde el inicio del ciclo en alta densidad (3,0 plantas m-2 sin adición de tallos laterales.Tomato cultivated in spring at a low plant density and then increasing the effective plant density by means of allowing side shoots to develop, when daily light integrals are high, appears a promising way of increasing spring-season tomato crop fruit number per square meter and to produce fruits of more uniform size; and has the added advantage that fewer plants have to be purchased per cropped area. The effects of side shoots on fruit uniformity and yield of tomato crop were evaluated in a trial carried out from March to July 1998 in a greenhouse in Almería (south-eastern Spain. The initial plant densities were 2.4 and 3.0 plants m-2, in the case of non-grafted plants, which had one main stem; and 1.2 plants m-2 in grafted plants, which had two main stems (2.4 shoots m-2 at setting. From April, by allowing side shoots to develop (when the treatment required, population densities of 2.4; 3.0 and 3.5 shoots m-2 (grafted and non-grafted plants were raised; and 3.0 non-grafted plants m-2 without side shoots during all cropping-season treatment was maintained. The results showed that grafted tomato plants set with two main stems presented the same final yield but produced fruits of more uniform size than non-grafted plants set with one main stem and double initial plant density. In a range from 2.4 to 3.5 shoots m-2, increasing shoot density increased early yield and produced fruits of more uniform size but presented no effect on the mean fruit weight and the final yield. The addition of side shoots (3.0 shoots m-2 was accompanied by a 20% loss of final yield but from 21% and 39% fewer non-grafted and grafted plants, respectively, and produced fruits of more uniform size, relative to the high initial density treatment (3.0 plants m-2 without side shoots.

  1. Effect of Bactericides on Population Sizes and Spread of Clavibacter michiganensis subsp. michiganensis on Tomatoes in the Greenhouse and on Disease Development and Crop Yield in the Field.

    Science.gov (United States)

    Hausbeck, M K; Bell, J; Medina-Mora, C; Podolsky, R; Fulbright, D W

    2000-01-01

    ABSTRACT Chemical applications, with the exception of mancozeb, reduced population sizes and spread of Clavibacter michiganensis subsp. michiganensis among tomato seedlings in the greenhouse and impacted subsequent plant development and yield in the field. While applications of copper hydroxide, copper hydroxide/mancozeb, copper hydroxide/mancozeb (premixed 12 h before spraying), streptomycin, and streptomycin/copper hydroxide to seedlings in the greenhouse did not differ significantly from the inoculated control, the trend was for these treatments to increase the survival of inoculated transplants in the field in comparison to the inoculated control. In the field, inoculated controls produced yields that were 63% (1995) and 51% (1996) of those produced by uninoculated controls. In both years, with the exception of mancozeb in 1995, all treatments resulted in yields similar to those obtained with the uninoculated control. Plant survival and yield in the field were severely affected when transplants had a pathogen population of >/= x 10(8) CFU/g of tissue. All treatments, with the exception of mancozeb, limited C. michiganensis subsp. michiganensis populations to <5.0 x 10(5). None of the treatments significantly reduced the incidence of fruit spotting compared with that of the inoculated control.

  2. On farm evaluation of the effect of low cost drip irrigation on water and crop productivity compared to conventional surface irrigation system

    Science.gov (United States)

    Maisiri, N.; Senzanje, A.; Rockstrom, J.; Twomlow, S. J.

    This on-farm research study was carried out at Zholube irrigation scheme in a semi-arid agro tropical climate of Zimbabwe to determine how low cost drip irrigation technologies compare with conventional surface irrigation systems in terms of water and crop productivity. A total of nine farmers who were practicing surface irrigation were chosen to participate in the study. The vegetable English giant rape ( Brassica napus) was grown under the two irrigation systems with three fertilizer treatments in each system: ordinary granular fertilizer, liquid fertilizer (fertigation) and the last treatment with no fertilizer. These trials were replicated three times in a randomized block design. Biometric parameters of leaf area index (LAI) and fresh weight of the produce, water use efficiency (WUE) were used to compare the performance of the two irrigation systems. A water balance of the inflows and outflows was kept for analysis of WUE. The economic profitability and the operation, maintenance and management requirements of the different systems were also evaluated. There was no significant difference in vegetable yield between the irrigation systems at 8.5 ton/ha for drip compared to 7.8 ton/ha in surface irrigation. There were significant increases in yields due to use of fertilizers. Drip irrigation used about 35% of the water used by the surface irrigation systems thus giving much higher water use efficiencies. The leaf area indices were comparable in both systems with the same fertilizer treatment ranging between 0.05 for surface without fertilizer to 6.8 for low cost drip with fertigation. Low cost drip systems did not reflect any labour saving especially when manually lifting the water into the drum compared to the use of siphons in surface irrigation systems. The gross margin level for surface irrigation was lower than for low cost drip irrigation but the gross margin to total variable cost ratio was higher in surface irrigation systems, which meant that surface

  3. Prospects: the tomato genome as a cornerstone for gene discovery

    Science.gov (United States)

    Those involved in the international tomato genome sequencing effort contributed to not only the development of an important genome sequence relevant to a major economic and nutritional crop, but also to the tomato experimental system as a model for plant biology. Without question, prior seminal work...

  4. Yield and quality criteria in organically and conventionally grown tomatoes in Turkey Produção e qualidade de tomates cultivados sob sistemas orgânico e convencional na Turquia

    Directory of Open Access Journals (Sweden)

    Ersin Polat

    2010-08-01

    Full Text Available The term 'organically grown food' denotes products that have been produced in accordance with the principles and practices of organic agriculture. The use of alternatives to synthetic fertilizers is an important issue in organic systems. A two-year field experiment to evaluate effects of organic fertilizers on the yield and quality of open field grown tomatoes (Lycopersicon esculentum Mill. was carried out in Southern Turkey in 2000 and 2001. Combinations of manure, blood flour and micronutrient preparations were used for fertilization, and conventional mineral fertilization was included as the control. Yield did not differ between the fertilization and the Conventional treatments in the first year of the study, but the highest yield was obtained from conventional in the second year. No differences were found between treatments in terms of fruit soluble sugar content or citric acid. The application of organic fertilizers positively affected the micronutritional element content of tomato fruits compared to the conventional treatment. Organic fertilization results in improved yield and fruit quality compared to conventional fertilization. In addition, organic fertilization should be supported in order to facilitate reuse and disposal of organic wastes and to maintain and/or increase soil fertility.O termo "alimentos cultivados organicamente" denota produtos que tenham sido produzidos em conformidade com os princípios e práticas da agricultura orgânica. O uso de alternativas para fertilizantes sintéticos é uma questão importante em sistemas orgânicos. Um experimento de campo foi conduzido no sul da Turquia em 2000 e 2001 para avaliar os efeitos da adubação orgânica na produtividade e na qualidade de tomates (Lycopersicon esculentum Mill, cultivados em campo aberto. Combinações de esterco, farinha de sangue e preparações de micronutrientes foram utilizados para a fertilização, e adubações minerais convencionais foram inclu

  5. Comportamento da cultura do tomateiro sob diferentes tensões de água no solo em ambiente protegido Behavior of tomato crop under different soil water tensions in a greenhouse

    Directory of Open Access Journals (Sweden)

    Nelson S. A. de Sá

    2005-09-01

    Full Text Available A cultura do tomateiro corresponde a uma atividade expressiva no Brasil, bastante exigente em água e, sob cultivo protegido, a irrigação deve ser usada para o suprimento hídrico total. O manejo adequado da irrigação é importante não apenas por suprir as necessidades hídricas das plantas mas, também, por minimizar problemas com doenças e lixiviação de nutrientes, além de gastos desnecessários com água e energia. Visando definir critérios para o manejo da irrigação, avaliou-se, através deste trabalho, o efeito de diferentes tensões de água no solo sobre o comportamento produtivo do tomateiro de crescimento indeterminado, híbrido Raísa N, em ambiente protegido, na região de Lavras, MG. O experimento foi instalado em casa de vegetação com delineamento em blocos casualizados, tendo quatro repetições. Os tratamentos foram constituídos de seis níveis de tensões de água no solo como indicativos do momento de irrigar. As tensões preestabelecidas foram 15, 30, 50, 70, 120 e 170 kPa e os resultados permitiram concluir-se que, para a obtenção de maiores produtividades de frutos totais, frutos comerciais e menor incidência de frutos com podridão apical, as irrigações devem ser realizadas quando as tensões de água no solo a 0,10 m de profundidade estiverem em torno de 80 kPa. Também foi possível observar que a eficiência no uso da água apresentou resposta linear crescente com o aumento da tensão de água no solo.The tomato crop corresponds to an expressive activity in Brazil, being a quite demanding crop in water and under greenhouse conditions the irrigation should be used for the total water supply. The appropriate irrigation management is not only an important factor to supply plant's water needs, but also to minimize problems with diseases, nutrient leaching as well as unnecessary expenses with water and energy. The purpose of this work was to evaluate the effect of different soil water tensions on the

  6. 农业废弃有机物基质配方对番茄生长及产量的影响%Influence of the formula of organic waste substrate from crop production on growth and yield of tomato

    Institute of Scientific and Technical Information of China (English)

    徐文俊; 程智慧; 孟焕文; 柴喜荣; 贾荣; 闫会玲

    2012-01-01

    【Objective】 The research was conducted to screen the formula of organic substrate based on the organic waste of crop production to set technical support for organic substrate cultivation of tomato.【Method】 Rice husk,corn straw,corn cob,wheat husk and wasted edible fungi culture medium were fermented separately and then composed to five multiple formula of organic substrates in volume proportion.The compositions were A(V(corn straw)∶V(wheat husk)∶V(wasted edible fungi culture medium)=2∶5∶3),B(V(rice husk)∶V(corn cob)∶V(wasted edible fungi culture medium)=5∶2∶3),C(V(rice husk)∶V(corn straw)∶V(wasted edible fungi culture medium)=5∶2∶3),D(V(rice husk)∶V(corn cob)∶V(wasted edible fungi culture medium)=3∶2∶5) and E(V(corn cob)∶V(wheat husk)∶V(wasted edible fungi culture medium)=2∶3∶5).Taking the typical organic ecotype substrate(V(peat)∶V(perlite)=2∶1) as the control(CK) and tomato cv.Jinpeng No.1 as the test plant,the physical and chemical property of the substrates and growth and yield of tomato were investigated.【Result】 The available nitrogen content in all organic substrate treatments is significantly lower but available phosphor and potassium significantly higher than that in CK.The unit weight,EC value and pH value of all organic substrate treatments are on the whole in the fitting range of tomato growth except that the total porosity is on the low side.The plant height,stem diameter,fresh mass and dry mass of tops and roots,single fruit weight,fruit number per plant,yield per plant and yield per plot of treatment B are the highest among all the treatments,close to or showing no significant difference with CK.Treatment C is just next to treatment B,however treatment E appears relatively poor.【Conclusion】 The physical and chemical property of the substrate treatment B fits the growth of tomato.There is no

  7. Mixture approach for optimizing lycopene extraction from tomato and tomato products.

    Science.gov (United States)

    Periago, María Jesús; Rincón, Francisco; Agüera, Maria Dolores; Ros, Gaspar

    2004-09-22

    A simple mixture process design based on the comparison of both quadratic and special cubic models and involving three mixture components (hexane/acetone/ethanol) as a solution for extracting lycopene from raw tomato, tomato sauce, and tomato paste was used to confirm the hypothesis that lycopene extraction rates are a function of the solvent used during the extraction process. Conventional criteria (p lycopene extraction was hexane, there was a positive secondary synergistic interaction of hexane with ethanol (all sample types) and with acetone (tomato paste samples); this suggests that a mixture including all three components is essential for optimizing the extraction process. The partial special cubic model yielded three stationary points, indicating the concentrations of hexane, acetone, and ethanol required to optimize lycopene extraction in raw tomato, tomato sauce, and paste.

  8. ECONOMIC EFFICIENCY IN TOMATOES PRODUCTION IN GREENHOUSES

    Directory of Open Access Journals (Sweden)

    A POPESCU

    2003-07-01

    Full Text Available This study aimed to appreciate the evolution of economic efficiency in tomatoes production in greenhouses within a private firm situated next to the capital. The firm owns 4 ha greenhouses and the weight of tomatoes crop in the cultivated area is just 38.75 %. In fact, during the last three years, the tomatoes cultivated surface has been diminished in favour of flowers production which, like tomatoes production is an important income source for any producer. The reduction of the tomatoes cultivated area was compensated by the increase of intensification grade using new high performance hybrids and modern technologies. Thus, the scientific production management has been looking for maintaining the total production at the same level from a year to another by an increased average tomatoes yield by 53.33 % . The continuous increase of farm input price has doubled the cost per surface unit and increased the cost per tomatoes kilogram by 33 %. The increase of tomatoes demand and of market price by 31 % have had a positive influence on the farm incomes which has doubled during the last three years. In the year 2000, the company has obtained USD 41,818 income/ha of which subtracting the related production cost we can easily get USD 4,815 profit/ha. The average profit rate recorded by the firm is 13 % in the period 2000-2002, when the study was made. As a conclusion, tomatoes production in greenhouses is a good deal. To keep a high economic efficiency, under the diminishing of the cultivated area, the producers have to increase average tomatoes production by using high performance technology based on high economic value hybrids.

  9. Compact tomato seedlings and plants upon overexpression of a tomato chromatin remodelling ATPase gene.

    Science.gov (United States)

    Folta, Adam; Bargsten, Joachim W; Bisseling, Ton; Nap, Jan-Peter; Mlynarova, Ludmila

    2016-02-01

    Control of plant growth is an important aspect of crop productivity and yield in agriculture. Overexpression of the AtCHR12/23 genes in Arabidopsis thaliana reduced growth habit without other morphological changes. These two genes encode Snf2 chromatin remodelling ATPases. Here, we translate this approach to the horticultural crop tomato (Solanum lycopersicum). We identified and cloned the single tomato ortholog of the two Arabidopsis Snf2 genes, designated SlCHR1. Transgenic tomato plants (cv. Micro-Tom) that constitutively overexpress the coding sequence of SlCHR1 show reduced growth in all developmental stages of tomato. This confirms that SlCHR1 combines the functions of both Arabidopsis genes in tomato. Compared to the wild type, the transgenic seedlings of tomato have significantly shorter roots, hypocotyls and reduced cotyledon size. Transgenic plants have a much more compact growth habit with markedly reduced plant height, severely compacted reproductive structures with smaller flowers and smaller fruits. The results indicate that either GMO-based or non-GMO-based approaches to modulate the expression of chromatin remodelling ATPase genes could develop into methods to control plant growth, for example to replace the use of chemical growth retardants. This approach is likely to be applicable and attractive for any crop for which growth habit reduction has added value.

  10. Chemical and sensory analysis of commercial tomato juices present on the Italian and Spanish markets.

    Science.gov (United States)

    Vallverdú-Queralt, Anna; Bendini, Alessandra; Tesini, Federica; Valli, Enrico; Lamuela-Raventos, Rosa Maria; Toschi, Tullia Gallina

    2013-02-06

    A quantitative descriptive analysis was developed to characterize the sensory quality of a set of 12 organic and conventional tomato juices sold in Spanish and Italian markets. The volatile compounds of tomato juices were also studied. Twelve sensory descriptors, selected by a trained panel, evaluated the sensory profile of the samples. Some tomato juices were characterized by dominant positive notes typical of tomatoes (tomato paste, vegetable notes), whereas others by negative sensory attributes (off-flavors, high intensity of acidity, and sweetness). The volatile pattern of the samples, studied by SPME/GC-MS, was correlated with the sensory results: basically, organic tomato juices were characterized by vegetable notes and higher volatile compounds than conventional samples, regardless of their geographical origin. Conventional tomato juices were grouped in a closer cluster, whereas organic tomato juices were more diversified. Moreover, "defective" samples showed higher amounts of 3-methyl-1-butanol.

  11. Molecular characterization of Yucatan tomato phytoplasma (Group ...

    African Journals Online (AJOL)

    Yomi

    2012-01-31

    Jan 31, 2012 ... Tomato (Lycopersicon esculentum) is an important vegetable crop in Mexico. Recently ... In addition, it will contribute to a greater knowledge of the genetic diversity of ... fruit are lower than expected, causing heavy economic losses. ..... Classification of plant-pathogenic mycoplasma-like organisms using.

  12. Assessing crop N status of fertigated vegetable crops using plant and soil monitoring techniques.

    Science.gov (United States)

    Peña-Fleitas, M T; Gallardo, M; Thompson, R B; Farneselli, M; Padilla, F M

    2015-11-01

    Evaluation of crop N status will assist optimal N management of intensive vegetable production. Simple procedures for monitoring crop N status such as petiole sap [NO 3(-)-N], leaf N content and soil solution [NO 3(-)] were evaluated with indeterminate tomato and muskmelon. Their sensitivity to assess crop N status throughout each crop was evaluated using linear regression analysis against nitrogen nutrition index (NNI) and crop N content. NNI is the ratio between the actual and the critical crop N contents (critical N content is the minimum N content necessary to achieve maximum growth), and is an established indicator of crop N status. Nutrient solutions with four different N concentrations (treatments N1-N4) were applied throughout each crop. Average applied N concentrations were 1, 5, 13 and 22 mmol L(-1) in tomato, and 2, 7, 13 and 21 mmol L(-1) in muskmelon. Respective rates of N were 23, 147, 421 and 672 kg N ha(-1) in tomato, and 28, 124, 245 and 380 kg N ha(-1) in muskmelon. For each N treatment in each crop, petiole sap [NO 3(-)-N] was relatively constant throughout the crop. During both crops, there were very significant (P 1. Relationships between petiole sap [NO 3(-)-N] with crop N content, and leaf N content with both NNI and crop N content had variable slopes and intercept values during the indeterminate tomato and the muskmelon crops. Soil solution [NO 3(-)] in the root zone was not a sensitive indicator of crop N status. Of the three systems examined for monitoring crop/soil N status, petiole sap [NO 3(-)-N] is suggested to be the most useful because of its sensitivity to crop N status and because it can be rapidly analysed on the farm.

  13. Philadelphia and the Tomato.

    Science.gov (United States)

    Smith, Andrew F.; Kling, Tatiana

    This booklet describes for elementary students the many contributions of people, traveling many places, over many years to bring the tomato to Philadelphia. The booklet includes the following: (1) "Introduction to the Tomato"; (2) "Where Does the Tomato Come From?"; (3) "The Spanish Tomato"; (4) "The Philadelphia…

  14. A dynamic tomato growth and yield model (TOMGRO).

    NARCIS (Netherlands)

    Jones, J.W.; Dayan, E.; Allen, L.H.; Keulen, van H.; Challa, H.

    1991-01-01

    Models of the greenhouse environment and of crops are needed to determine optimal strategies for environment control in regions where new greenhouse industries are developing. In this research, a physiological model of tomato crop development and yield was developed. A series of differential

  15. Sustainability of European winter wheat- and maize-based cropping systems: Economic, environmental and social ex-post assessment of conventional and IPM-based systems

    NARCIS (Netherlands)

    Vasileiadis, V.P.; Dachbrodt-saaydeh, S.; Kudsk, P.; Colnenne-David, C.; Leprince, F.; Holb, I.J.; Kierzek, R.; Furlan, L.; Loddo, D.; Melander, B.; Jørgensen, L.N.; Newton, A.C.; Toque, C.; Dijk, van W.; Lefebvre, M.; Benezit, M.; Sattin, M.

    2017-01-01

    In order to ensure higher sustainability of winter wheat and maize production in Europe, cropping systems featuring different levels of Integrated Pest Management (IPM) need to be tested in the field and validated for their sustainability before being adopted by farmers. However, the sustainability

  16. Response of Tomato on Calcareous Soils to Different Seedbed Phosphorus Application Rates

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiao-Sheng; LIAO Hong; CHEN Qing; P. CHRISTIE; LI Xiao-Lin; ZHANG Fu-Suo

    2007-01-01

    Field experiments were conducted with five rates (0, 75, 150, 225, and 450 kg P2O5 ha-1) of seedbed P fertilizer application to investigate the yield of tomato in response to fertilizer P rate on calcareous soils with widely different levels of Olsen P (13-142 mg kg-1) at 15 sites in some suburban counties of Beijing in 1999. Under the condition of no P fertilizer application, tomato yield generally increased with an increase in soil test P levels, and the agronomic level for soil testing P measured with Olsen method was 50 or 82 mg kg-1 soil to achieve 85% or 95% of maximum tomato yield, respectively. With regard to marketable yield, in the fields where Olsen-P levels were < 50 mg kg-1, noticeable responses to applied P were observed. On the basis of a linear plateau regression, the optimum seedbed P application rate in the P-insufficient fields was 125 kg P2O5 ha-1 or about 1.5-2 times the P removal from harvested tomato plants. In contrast, in fields with moderate (50 < Olsen P < 90 mg kg-1) or high (Olsen P > 90 mg kg-1) available P, there was no marked effect on tomato fruit yield. Field survey data indicated that in most fields with conventional P management, a P surplus typically occurred. Thus, once the soil test P level reached the optimum for crop yield, it was recommended that P fertilizer application be restricted or eliminated to minimize negative environmental effects.

  17. Quality of tomato seedling in application bioproducts

    OpenAIRE

    BOTEVA, Hriska

    2014-01-01

    The study was performed during the period 2009 - 2011 in the “Maritsa” Vegetable Crops Research Institute, Plovdiv. The effect of organic products Baikal EM – 1У, Bioglobin and Biolan on the quality of seedlings from tomato, variety Yana was studied in unheated glasshouses ro-ON type. Plants were grown on two substrates: peat-perlite substrate and substrate with Lumbrikal. The post-effect of the applied bioproducts on the plant productivity was studied in field conditions. Tomato seeds from Y...

  18. Targeting chitinase gene of Helicoverpa armigera by host-induced RNA interference confers insect resistance in tobacco and tomato.

    Science.gov (United States)

    Mamta; Reddy, K R K; Rajam, M V

    2016-02-01

    Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is a devastating agricultural insect pest with broad spectrum of host range, causing million dollars crop loss annually. Limitations in the present conventional and transgenic approaches have made it crucial to develop sustainable and environmental friendly methods for crop improvement. In the present study, host-induced RNA interference (HI-RNAi) approach was used to develop H. armigera resistant tobacco and tomato plants. Chitinase (HaCHI) gene, critically required for insect molting and metamorphosis was selected as a potential target. Hair-pin RNAi construct was prepared from the conserved off-target free partial HaCHI gene sequence and was used to generate several HaCHI-RNAi tobacco and tomato plants. Northern hybridization confirmed the production of HaCHI gene-specific siRNAs in HaCHI-RNAi tobacco and tomato lines. Continuous feeding on leaves of RNAi lines drastically reduced the target gene transcripts and consequently, affected the overall growth and survival of H. armigera. Various developmental deformities were also manifested in H. armigera larvae after feeding on the leaves of RNAi lines. These results demonstrated the role of chitinase in insect development and potential of HI-RNAi for effective management of H. armigera.

  19. Evaluation of the Water Requirements for a Greenhouse Tomato Crop using the Priestley-Taylor Method Evaluación del Consumo de Agua de un Cultivo de Tomate en Invernadero Usando el Método de Priestley-Taylor

    Directory of Open Access Journals (Sweden)

    Héctor Valdés-Gómez

    2009-03-01

    Full Text Available The Priestley-Taylor (PT model was evaluated for estimating the real evapotranspiration (ETreal of a drip-irrigated greenhouse tomato (Lycopersicon esculentum Mill. crop. The net radiation incorporated in the PT model was estimated using meteorological variables. For this experiment, an automatic weather station (AWS was installed inside the greenhouse to measure solar radiation (Rgi, net radiation (Rn, air temperature (Ta and relative humidity (RH. Another AWS was installed over a grass cover to measure atmospheric conditions outside the greenhouse. The experiment was carried out at the Panguilemo experimental station (35°23' S, 71°40' W, 110 m.a.s.l. from August to December 2000. The PT model was evaluated using the ETreal obtained from the water balance (WB method. In this case, values of ETreal by PT model were calculated using: a Rgi and soil heat flux (G = 0; b Rgi and G ≠ 0; c solar radiation measured outside the greenhouse (Rge and G = 0; and d Rge and G ≠ 0. For these cases, results indicated that PT model was able to compute ETreal with errors less than 5%. Also, Rn was calculated with a relative absolute error and a mean deviation lower than 6% and 0.07 mm d-1, respectively, using Rgi or Rge. Daily soil heat flux values equal to zero did not affect the calculation of ETreal values. Thus, the PT model evaluated in this study could be used for scheduling irrigation for a greenhouse tomato crop, using internal measurements of air temperature and relative humidity, and external measurements of solar radiation. In this case, PT model predicted the ETreal with an error of 6.1%.Se evaluó el comportamiento del modelo de Priestley-Taylor (PT para la estimación de la evapotranspiración real (ETreal de un cultivo de tomates (Lycopersicon esculentum Mill. en condiciones de invernadero. La radiación neta incorporada en el modelo de PT fue calculada usando variables meteorológicas clásicas. Para este experimento, una estación meteorol

  20. Genetic analysis of reproductive development in tomato.

    Science.gov (United States)

    Lozano, Rafael; Giménez, Estela; Cara, Beatriz; Capel, Juan; Angosto, Trinidad

    2009-01-01

    Besides being an important commercial crop, tomato (Solanum lycopersicum L.) constitutes a model species for the study of plant developmental processes. Current research tends to combine classic disciplines such as physiology and genetics with modern approaches coming from molecular biology and genomics with a view to elucidating the biological mechanisms underlying plant architecture, floral transition and development of flowers and fruits. Comparative and functional analyses of tomato regulatory genes such as LATERAL SUPPRESSOR (LS), SELF PRUNING (SP), SINGLE FLOWER TRUSS (SFT) and FALSIFLORA (FA) have revealed mechanisms involved in shoot development and flowering time which are conserved among Arabidopsis, tomato and other plant species. Furthermore, several regulatory genes encoding transcription factors have been characterized as responsible for singular features of vegetative and reproductive development of tomato. Thus, the sympodial growth habit seems to require a specific control of the developmental fate followed by shoot meristems. In this process, novel genetic and molecular interactions involving SP, SFT and FA genes would be essential. Also this latter, but mainly ANANTHA (AN) and COMPOUND INFLORESCENCE (S) have recently been found to regulate the inflorescence architecture of the tomato. Concerning fruit development, genetic and molecular analyses of new genes such as fw2.2, FASCIATED, OVATE and SUN have proved their contribution to the domestication process and most importantly, their function as key regulators of fruit size and shape variation. Tomato ripening is also being elucidated thanks to the characterization of regulatory genes such as RIPENING INHIBITOR (RIN), NON-RIPENING (NOR), TDR4 and COLORLESS NON-RIPENING (CNR), which have been found to control early stages of fruit development and maturation. At the same time, much research is dedicated to isolating the targets of the ripening regulators, as well as the key genes promoting the

  1. Aplicação de modelos múltiplos na determinação de níveis de prejuízo para a interação Solanum americanum e tomate de indústria Evaluation of economic levels for the Solanum americanum x tomato crop interaction using multiple models

    Directory of Open Access Journals (Sweden)

    J.M Portugal

    2011-12-01

    Full Text Available Este estudo apresenta um conceito unificador, que conjuga as teorias de densidade crítica e período crítico. Hipotetiza-se que não existe um valor fixo para a densidade crítica e para o período crítico, mas que os seus valores são interdependentes. A demonstração dessa hipótese é fundamentada em estudos de interferência entre Solanum americanum e o tomateiro. Aos resultados da produção de cinco ensaios, aplicaram-se modelos múltiplos de regressão, tendo como variáveis independentes a densidade e o número de dias de convivência entre a infestante e a cultura. A aplicação do Nível de Tolerância (NT à representação gráfica num plano das equações estimadas a partir de modelos múltiplos permite responder à questão: Quanto tempo pode permanecer uma determinada infestação de Solanum americanum sem causar prejuízo na cultura do tomateiro? É ainda possível verificar que a variação dos custos do controle e dos preços da cultura faz variar o NT e, concomitantemente, o tempo admissível, sem causar prejuízo, do Solanum americanum na cultura do tomateiro.This study introduces a unifying concept combining the theory of critical density and critical period of weed infestation. It is hypothesized that these two periods are interdependent, rather than fixed values. The hypothesis is based on studies of interference between Solanum americanum and the tomato crop. Multiple regression models were applied to the results of five field trials. The independent variables were density and number of days of weed and culture cohabitation. Applying the Tolerance Level (TL to the graphic representation (2D of the equations estimated from multiple non-linear models allowed answering the following question: how long does a particular infestation of Solanum americanum remain without causing injury to the tomato crop? It was also possible to conclude that the variation of control costs and crop prices changes the TL values, concomitantly

  2. Determination of polyphenol contents and antioxidant capacity of no-alcoholic red grape products (vitis labrusca from conventional and organic crops

    Directory of Open Access Journals (Sweden)

    Michel Mansur Machado

    2011-01-01

    Full Text Available The polyphenol contents and antioxidant capacity of Brazilian red grape juices and wine vinegars were analyzed. Additionally, it was analyzed the human polyphenol absorption and acute effect in plasmatic oxidative metabolism biomarkers after juice ingestion. The organic Bordo grape juice (GBO presented a higher level of trans-resveratrol, quercitin, rutin, gallic acid, caffeic acid and total flavonoids then other juices and vinegars as well as antioxidant capacity. The plasmatic polyphenol increased 27.2% after GBO juice ingestion. The results showed that juices and vinegars from Brazilian crops present similar chemical and functional properties described in studies performed in other countries.

  3. Thrips-transmitted Viruses Infect a Number of Florida Crops

    Science.gov (United States)

    The ilarviruses Tomato necrotic streak virus and Tobacco streak virus are present in south Florida. Both species cause economically significant disease in vegetable crop. Control of these viruses makes use of integrated pest management approaches....

  4. Integrated pest management of the southern green stinkbug, Nezara viridula (Hemiptera: Pentatomidae) on tomato in North Florida

    Science.gov (United States)

    The Southern Green Stinkbug, Nezara viridula is a serious insect pest of tomatoes in north Florida. We evaluated three trap crops and three refuge crops to investigate their potential for IPM of N. viridula. The experimental trap crops and refuge crops were, striped sunflower, WGF sorghum and brown ...

  5. Effect of N and K Fertilizers on Yield and Quality of Greenhouse Vegetable Crops

    Institute of Scientific and Technical Information of China (English)

    LIU Zhao-Hui; JIANG Li-Hua; LI Xiao-Lin; R. H(A)RDTER; ZHANG Wen-Jun; ZHANG Yu-Lan; ZHENG Dong-Feng

    2008-01-01

    The application of large amounts of fertilizers, a conventional practice in northern China for the production of vegetable crops, generally leads to substantial accumulation of soil nutrients within a relatively short period of time. A fixed field experiment was designed to study the effects of nitrogen (N) and potassium (K) fertilizers applicd to optimize the yield and quality of typical vegetable crops. Application of N and K fertilizers significantly increased the yields of kidney bean.The largest yields were obtained in the first and second years after application of 1 500 kg N and 300 kg K2O ha-1. In the third year, however, there was a general decline in yields. Maximum yields occurred whcn intermediate rates of N and K (750 kg N and 300 kg K2O ha-1) were applied. However, no significant differences were observed in the concentrations of vitamin C (VC) in kidney bean among different years and various rates of fertilizer treatments. Yields of tomato grown in rotation after kidney bean showed significant responses to the application of N and K in the first year. In the second year,the yields of tomato were much lower. This suggested that the application of N fertilizer did not have any effect upon tomato yield, whereas application of K fertilizer did increase the yield. Application of K fertilizer was often associated with increased sugar concentrations.

  6. Tomato as a Source of Carotenoids and Polyphenols Targeted to Cancer Prevention

    Science.gov (United States)

    Martí, Raúl; Roselló, Salvador; Cebolla-Cornejo, Jaime

    2016-01-01

    A diet rich in vegetables has been associated with a reduced risk of many diseases related to aging and modern lifestyle. Over the past several decades, many researches have pointed out the direct relation between the intake of bioactive compounds present in tomato and a reduced risk of suffering different types of cancer. These bioactive constituents comprise phytochemicals such as carotenoids and polyphenols. The direct intake of these chemoprotective molecules seems to show higher efficiencies when they are ingested in its natural biological matrix than when they are ingested isolated or in dietary supplements. Consequently, there is a growing trend for improvement of the contents of these bioactive compounds in foods. The control of growing environment and processing conditions can ensure the maximum potential accumulation or moderate the loss of bioactive compounds, but the best results are obtained developing new varieties via plant breeding. The modification of single steps of metabolic pathways or their regulation via conventional breeding or genetic engineering has offered excellent results in crops such as tomato. In this review, we analyse the potential of tomato as source of the bioactive constituents with cancer-preventive properties and the result of modern breeding programs as a strategy to increase the levels of these compounds in the diet. PMID:27331820

  7. Survey of tomato diseases in Cameroon

    Directory of Open Access Journals (Sweden)

    Fontem, DA.

    1993-01-01

    Full Text Available Tomato (Lycopersicon esculentum Mill. is the most widely cultivated field vegetable crop in Cameroon. On-farm surveys were undertaken from November 1988 to October 1991 to identify nursery and field diseases in major tomato producing areas of Cameroon, Damping-off and seedling blights were the main seedling diseases. Of the eleven diseases observed in the field, the most widely distributed and severe on the foliage and fruits were early (Alternaria solani and late (Phytophthora infestans blights. Late blight was the most severe disease in the wet season while early blight was most severe in the dry season. Nine pathogens were associated with various fruit rots. This study indicates the need for an identification of appropriate control methods for early and late blights of tomato in Cameroon.

  8. Production cost analysis and use of pesticides in the transgenic and conventional corn crop [Zea mays (L.)] in the valley of San Juan, Tolima.

    Science.gov (United States)

    Méndez, Kelly Avila; Chaparro Giraldo, Alejandro; Moreno, Giovanni Reyes; Castro, Carlos Silva

    2011-01-01

    A survey of 10 producers of conventional corn (Hybrids PAC 105 and Maximus) and 10 producers of transgenic corn (Pioneer Hybrid 30T17) was carried out in the municipality of Valle de San Juan in the territorial division of Tolima (Colombia), in order to analyze the differences in production costs and environmental impacts of these two agricultural technologies.  The environmental impacts were determined by calculating the field "Environmental Index Quotient" (EIQ). In the production cost analysis, a difference of 15% was found in benefit of the transgenic technology. The structure of costs of the transgenic technology was benefited by the reduced use of pesticides (insecticides and herbicides). In regards to production, the transgenic technology showed a greater yield, 5.22 ton/ha in comparison to 4.25 ton/ha the conventional technology, thus a 22% difference in yield. Finally, the EIQ calculation showed quantitative differences of 196.12 for the conventional technology (EIQ insecticides 165.14 + EIQ herbicides 30.98), while the transgenic technology was of 4.24 (EIQ insecticides 0 + EIQ herbicides 4.24). These results show a minor environmental impact when using the transgenic technology in comparison to the conventional technology, in regards to the use of insecticides and herbicides in a temporal, spatial and genotypical context analysis. :

  9. Efectos de diferentes agroecosistemas en la dinámica de nitrógeno, fósforo y potasio en un cultivo de tomate Effects of different agro-ecosystems in the dynamic of nitrogen, phosphorous, and potassium in the tomato crop

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Bouzo

    Full Text Available El objetivo de este trabajo fue evaluar la dinámica del nitrógeno, fósforo y potasio en cultivos de tomate y suelos en diferentes agroecosistemas. El trabajo consistió en estudiar el efecto de tres agroecosistemas: agrícola (T1, natural (T2 y hortícola (T3. Estos agroecosistemas se caracterizaron por la secuencia de los cultivos de maíz y trigo durante 8 años (T1, vegetación natural de gramíneas (T2 y rotaciones de cultivos hortícolas durante 20 años (T3. El estudio fue realizado en Santa Fe, Argentina (31° 15' S, 60° 50' W entre 2009 y 2010, habiéndose utilizado un cultivar de tomate híbrido redondo de crecimiento indeterminado. La concentración de N-NO3- en los suelos tuvo un valor alto (The objective ofthis research was to evaluate the dynamics of nitrogen, phosphorous, and potassium in tomato crops and in different soils of agro-ecosystems. The research consisted of studying the effect of three agro-ecosystems: 1 agricultural (T1, natural (T2 and horticultural (T3. These agro-ecosystems are characterized by the sequence of the maize and wheat crops during 8 years (T1, natural vegetation of grasses (T2, and rotation of horticultural crops during 20 years (T3. The study was done in Santa Fe, Argentina (31° 15' S, 60° 50' W between 2009 and 2010, having used a hybrid tomato crop, round with indeterminate growth. The concentration of N-NO3- in the soils had a high value (< 65 ppm at the beginning of the crop in the three agro-ecosystems. The concentration of P was also very high (< 250 ppm in the horticultural agro-ecosystem (T3 and less than the rest, with approximately 50 ppm for T1 and 150 ppm for T2. The same occurred in this agro-ecosystem with K. The natural agro-ecosystem (T2 was the one that had the lowest concentration of P in the soil. The concentrations of N, P, and K resulted with highly significant differences (p≤ 0.01 in the concentrations of the stalks of the plants coming from the three agro

  10. Comparison of Soil Fauna (Oribatids and Enchytraeids)Between Conventional and Organic (Tillage and No—Tillage Practices)Farming Crop Fields in Japan

    Institute of Scientific and Technical Information of China (English)

    M.FUJITA; S.FUJIYAMA

    2001-01-01

    The major soil animal groups,enchyraeid worms and oribatid mites,were compared in the abundance and diversity between conventional fields(CT)and organic farming fields with tillage(OT) or no-tillage(ON)practices,The values of abundance,species richness,diversity and evenness were significantly larger in OT and ON than in CT,indicating that the abundance and diversity in organic farming fields were greater than those in conventional farming,The community structure of enchytraeid genera was different between OT and ON,Enchytraeus was the most abundant in OT ,while Fridericia in ON,The abundance of oribatids in OT was similar th that in ON,while the species richness and diversity in the former were smaller,These results suggeste that no-tilage practice under organic management might comtribute to the improvement in quality of soil mesofauna.

  11. Changes in volatile production during an infection of tomato plants by Botrytis cinerea

    NARCIS (Netherlands)

    Jansen, R.M.C.; Miebach, M.; Kleist, E.; Henten, van E.J.; Wildt, J.

    2006-01-01

    Botrytis blight caused by the fungus Botrytis cinerea is probably the most common disease of greenhouse-grown crops like tomato. Botrytis blight in tomato plants is mainly detected by visual inspection or destructive biochemical and molecular determinations. These methods are time consuming and not

  12. Variation among volatile profiles induced by Botrytis cinerea infection of tomato plants

    NARCIS (Netherlands)

    Jansen, R.M.C.

    2007-01-01

    Botrytis blight caused by the fungus Botrytis cinerea is probably the most common disease of greenhouse-grown crops like tomato. Botrytis blight in tomato plants is mainly detected by visual inspection or destructive biochemical and molecular determinations. These methods are time consuming and not

  13. Molecular characterization of Xanthomonas strains responsible for bacterial leaf spot of tomato in Ethiopia

    Science.gov (United States)

    Bacterial spot of tomato (BST) is a major constraint to tomato production in Ethiopia and many other countries leading to significant crop losses. In the present study, using pathogenicity tests, sensitivity to copper and streptomycin, and multilocus sequence analysis, a diverse group of Xanthomonas...

  14. Yield of glyphosate-resistant sugar beets and efficiency of weed management systems with glyphosate and conventional herbicides under German and Polish crop production.

    Science.gov (United States)

    Nichterlein, Henrike; Matzk, Anja; Kordas, Leszek; Kraus, Josef; Stibbe, Carsten

    2013-08-01

    In sugar beet production, weed control is one of the most important and most expensive practices to ensure yield. Since glyphosate-resistant sugar beets are not yet approved for cultivation in the EU, little commercial experience exists with these sugar beets in Europe. Experimental field trials were conducted at five environments (Germany, Poland, 2010, 2011) to compare the effects of glyphosate with the effects of conventional weed control programs on the development of weeds, weed control efficiency and yield. The results show that the glyphosate weed control programs compared to the conventional methods decreased not only the number of herbicide applications but equally in magnitude decreased the dosage of active ingredients. The results also showed effective weed control with glyphosate when the weed covering was greater and sugar beets had a later growth stage of four true leaves. Glyphosate-resistant sugar beets applied with the glyphosate herbicide two or three times had an increase in white sugar yield from 4 to 18 % in comparison to the high dosage conventional herbicide systems. In summary, under glyphosate management sugar beets can positively contribute to the increasingly demanding requirements regarding efficient sugar beet cultivation and to the demands by society and politics to reduce the use of chemical plant protection products in the environment.

  15. Fruit preferential activity of the tomato RIP1 gene promoter in transgenic tomato and Arabidopsis.

    Science.gov (United States)

    Agarwal, Priyanka; Kumar, Rahul; Pareek, Amit; Sharma, Arun K

    2017-02-01

    Isolation and functional characterization of tissue- and stage-specific gene promoters is beneficial for genetic improvement of economically important crops. Here, we have characterized a putative promoter of a ripening-induced gene RIP1 (Ripening induced protein 1) in tomato. Quantification of the transcript level of RIP1 showed that its expression is fruit preferential, with maximum accumulation in red ripe fruits. To test the promoter activity, we made a reporter construct by cloning 1450 bp putative RIP1 promoter driving the GUS (ß-glucuronidase) gene expression and generated stable transgenic lines in tomato and Arabidopsis. Histochemical and fluorometric assays validated the fruit-specific expression of RIP1 as the highest GUS activity was found in red ripe tomatoes. Similarly, we detected high levels of GUS activity in the siliques of Arabidopsis. On the contrary, weak GUS activity was found in the flower buds in both tomato and Arabidopsis. To characterize the specific regions of the RIP1 promoter that might be essential for its maximum activity and specificity in fruits, we made stable transgenic lines of tomato and Arabidopsis with 5'-deletion constructs. Characterization of these transgenic plants showed that the full length promoter is essential for its function. Overall, we report the identification and characterization of a ripening-induced promoter of tomato, which would be useful for the controlled manipulation of the ripening-related agronomic traits in genetic manipulation studies in future.

  16. A Novel Vision Sensing System for Tomato Quality Detection

    Directory of Open Access Journals (Sweden)

    Satyam Srivastava

    2014-01-01

    Full Text Available Producing tomato is a daunting task as the crop of tomato is exposed to attacks from various microorganisms. The symptoms of the attacks are usually changed in color, bacterial spots, special kind of specks, and sunken areas with concentric rings having different colors on the tomato outer surface. This paper addresses a vision sensing based system for tomato quality inspection. A novel approach has been developed for tomato fruit detection and disease detection. Developed system consists of USB based camera module having 12.0 megapixel interfaced with ARM-9 processor. Zigbee module has been interfaced with developed system for wireless transmission from host system to PC based server for further processing. Algorithm development consists of three major steps, preprocessing steps like noise rejection, segmentation and scaling, classification and recognition, and automatic disease detection and classification. Tomato samples have been collected from local market and data acquisition has been performed for data base preparation and various processing steps. Developed system can detect as well as classify the various diseases in tomato samples. Various pattern recognition and soft computing techniques have been implemented for data analysis as well as different parameters prediction like shelf life of the tomato, quality index based on disease detection and classification, freshness detection, maturity index detection, and different suggestions for detected diseases. Results are validated with aroma sensing technique using commercial Alpha Mos 3000 system. Accuracy has been calculated from extracted results, which is around 92%.

  17. A Novel Vision Sensing System for Tomato Quality Detection.

    Science.gov (United States)

    Srivastava, Satyam; Boyat, Sachin; Sadistap, Shashikant

    2014-01-01

    Producing tomato is a daunting task as the crop of tomato is exposed to attacks from various microorganisms. The symptoms of the attacks are usually changed in color, bacterial spots, special kind of specks, and sunken areas with concentric rings having different colors on the tomato outer surface. This paper addresses a vision sensing based system for tomato quality inspection. A novel approach has been developed for tomato fruit detection and disease detection. Developed system consists of USB based camera module having 12.0 megapixel interfaced with ARM-9 processor. Zigbee module has been interfaced with developed system for wireless transmission from host system to PC based server for further processing. Algorithm development consists of three major steps, preprocessing steps like noise rejection, segmentation and scaling, classification and recognition, and automatic disease detection and classification. Tomato samples have been collected from local market and data acquisition has been performed for data base preparation and various processing steps. Developed system can detect as well as classify the various diseases in tomato samples. Various pattern recognition and soft computing techniques have been implemented for data analysis as well as different parameters prediction like shelf life of the tomato, quality index based on disease detection and classification, freshness detection, maturity index detection, and different suggestions for detected diseases. Results are validated with aroma sensing technique using commercial Alpha Mos 3000 system. Accuracy has been calculated from extracted results, which is around 92%.

  18. Comparison of Soil Fauna (Oribatids and Enchytraeids){1mm BetweenConventional and Organic (Tillage and No-1mm TillagePractices) Farming Crop Fields in Japan

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The major soil animal groups, enchytraeid worms and oribatid mites,were compared in the abundance and diversity between conventionalfields (CT) and organic farming fields with tillage (OT) or no-tillage(ON) practices. The values of abundance, species richness, diversityand evenness were significantly larger in OT and ON than in CT,indicating that the abundance and diversity in organic farming fieldswere greater than those in conventional farming. The communitystructure of enchytraeid genera was different between OT and ON.{ Enchytraeus was the most abundant in OT, whileFridericia in ON. The abundance of oribatids in OT was similarto that in ON, while the species richness and diversity in the formerwere smaller. These results suggested that no-tillage practice underorganic management might contribute to the improvement in quality ofsoil mesofauna.

  19. Improvement of tomato local varieties by grafting in organic farming

    Science.gov (United States)

    Moreno, Marta M.; Villena, Jaime; Moreno, Carmen; García, Arántzazu M.; Mancebo, Ignacio; Meco, Ramón

    2015-04-01

    Grafting is the union of two or more pieces of living plant tissue that grow as a single plant. The early use of grafted vegetables was associated with protected cultivation which involves successive cropping (Lee et al., 2010). For this reason, in the past, grafting was used with vegetable crops to limit the effects of soil-borne diseases. However, the reasons for grafting as well as the kinds of vegetable grafted have increased considerably over the years. In tomato (Solanum lycopersicum L.), one of the most important horticultural crops in the world, the effect of grafting has also been widely studied. These effects on commercial tomato varieties can be summarized in increasing plant vigor and crop yield or inducing tolerance to abiotic stresses, although the effects on tomato fruit quality or on the sensory properties are not so patent (David et al., 2008). However, a few studies about the effect of grafting on local tomato varieties, which are especially recommended for organic production in spite of their lower yields in many cases, have been developed. In this work we evaluated the effect of grafting on tomato local varieties under organic management using vigorous commercial rootstocks, and aspects related to vigor, yield and tomato fruit composition were analyzed. In general terms, grafting increased the plant vigor, the crop yield and the fruit antioxidant content, although no modification of morphological fruit attributes was observed. Keywords: grafting, Solanum lycopersicum L., local varieties, organic farming. References: Davis A.R., Perkins-Veazie P., Hassell R., Levi A., King S.R., Zhang X. 2008. Grafting effects on vegetable quality. HortScience 43(6): 1670-1671. Lee J.M., Kubota C., Tsao S.J., Bie Z., Hoyos-Echevarría P., Morra L., Oda M. 2010. Current status of vegetable grafting: Diffusion, grafting techniques, automation. Scientia Horticulturae 127: 93-105.

  20. Analysis of Furaneol in tomato using dynamic headspace sampling with sodium sulfate.

    Science.gov (United States)

    Buttery, R G; Takeoka, G R; Naim, M; Rabinowitch, H; Nam, Y

    2001-09-01

    High-flow dynamic headspace sampling with excess anhydrous sodium sulfate was found to be an effective method of isolating Furaneol from fresh tomatoes. Quantitative analysis was carried out by gas chromatography using maltol as internal standard. Furaneol was found in the highest concentrations (660-1100 ppb) in the summer crop of home-grown tomatoes and in some of the greenhouse hydroponically grown tomatoes, which are ripened on the plant before being transported to the supermarkets. Furaneol was found in the lowest concentrations (38-180 ppb) in the common ethylene-ripened, field-grown, supermarket tomatoes.

  1. Impact of the reusing of food manufacturing wastewater for irrigation in a closed system on the microbiological quality of the food crops.

    Science.gov (United States)

    Beneduce, Luciano; Gatta, Giuseppe; Bevilacqua, Antonio; Libutti, Angela; Tarantino, Emanuele; Bellucci, Micol; Troiano, Eleonora; Spano, Giuseppe

    2017-11-02

    In order to evaluate if the reuse of food industry treated wastewater is compatible for irrigation of food crops, without increased health risk, in the present study a cropping system, in which ground water and treated wastewater were used for irrigation of tomato and broccoli, during consecutive crop seasons was monitored. Water, crop environment and final products were monitored for microbial indicators and pathogenic bacteria, by conventional and molecular methods. The microbial quality of the irrigation waters influenced sporadically the presence of microbial indicators in soil. No water sample was found positive for pathogenic bacteria, independently from the source. Salmonella spp. and Listeria monocytogenes were detected in soil samples, independently from the irrigation water source. No pathogen was found to contaminate tomato plants, while Listeria monocytogenes and E. coli O157:H7 were detected on broccoli plant, but when final produce were harvested, no pathogen was detected on edible part. The level of microbial indicators and detection of pathogenic bacteria in field and plant was not dependent upon wastewater used. Our results, suggest that reuse of food industry wastewater for irrigation of agricultural crop can be applied without significant increase of potential health risk related to microbial quality. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Variation in antibiosis ability, against potato pathogens, of bacterial communities recovered from the endo- and exoroots of potato crops produced under conventional versus minimum tillage systems.

    Science.gov (United States)

    Sturz, A V; Peters, R D; Carter, M R; Sanderson, J B; Matheson, B G; Christie, B R

    2005-08-01

    The culturable component of bacterial communities found in the endoroot and associated exoroot (root zone soil) was examined in potatoes (Solanum tuberosum L.) grown under either conventional or minimum tillage systems. Bacterial species--abundance relationships were determined and in vitro antibiosis ability investigated to discover whether tillage practice or bacteria source (endo- or exoroot) influenced bacterial community structure and functional versatility. Antibiosis abilities against Phytophthora erythroseptica Pethyb. (causal agent of pink rot of potatoes), Streptomyces scabies (Thaxt.) Waksm. and Henrici) (causal agent of potato common scab), and Fusarium oxysporum Schlecht. Emend. Snyder and Hansen (causal agent of fusarium potato wilt) were selected as indicators of functional versatility. Bacterial community species richness and diversity indices were significantly greater (P = 0.001) in the exoroot than in the endoroot. While both endo- and exoroot communities possessed antibiosis ability against the phytopathogens tested, a significantly greater proportion (P = 0.0001) of the endoroot population demonstrated antibiosis ability than its exoroot counterpart against P. erythroseptica and F. oxysporum. Tillage regime had no significant influence on species-abundance relationships in the endo- or exoroot but did influence the relative antibiosis ability of bacteria in in vitro challenges against S. scabies, where bacteria sourced from minimum tillage systems were more likely to have antibiosis ability (P = 0.0151). We postulate that the difference in the frequency of isolates with antibiosis ability among endoroot versus exoroot populations points to the adaptation of endophytic bacterial communities that favour plant host defence against pathogens that attack the host systemically.

  3. Practical improvements in soil redox potential (Eh) measurement for characterisation of soil properties. Application for comparison of conventional and conservation agriculture cropping systems.

    Science.gov (United States)

    Husson, Olivier; Husson, Benoit; Brunet, Alexandre; Babre, Daniel; Alary, Karine; Sarthou, Jean-Pierre; Charpentier, Hubert; Durand, Michel; Benada, Jaroslav; Henry, Marc

    2016-02-04

    The soil redox potential (Eh) can provide essential information to characterise soil conditions. In practice, however, numerous problems may arise regarding: (i) Eh determination in soils, especially aerobic soils, e.g. variations in the instrumentation and methodology for Eh measurement, high spatial and temporal Eh variability in soils, irreversibility of the redox reaction at the surface electrode, chemical disequilibrium; and (ii) measurement interpretation. This study aimed at developing a standardised method for redox potential measurement in soils, in order to use Eh as a soil quality indicator. This paper presents practical improvements in soil Eh measurement, especially regarding the control of electromagnetic perturbations, electrode choice and preparation, soil sample preparation (drying procedure) and soil:water extraction rate. The repeatability and reproducibility of the measurement method developed are highlighted. The use of Eh corrected at pH7, pe+pH or rH2, which are equivalent notions, is proposed to facilitate interpretation of the results. The application of this Eh measurement method allows characterisation of soil conditions with sufficient repeatability, reproducibility and accuracy to demonstrate that conservation agriculture systems positively alter the protonic and electronic balance of soil as compared to conventional systems.

  4. Practical improvements in soil redox potential (Eh) measurement for characterisation of soil properties. Application for comparison of conventional and conservation agriculture cropping systems

    Energy Technology Data Exchange (ETDEWEB)

    Husson, Olivier, E-mail: Olivier.husson@cirad.fr [CIRAD/PERSYST/UPR 115 AIDA and AfricaRice Centre, 01 BP 2031 Cotonou (Benin); Husson, Benoit, E-mail: bhusson@ideeaquaculture.com [IDEEAQUACULTURE, Parc Euromédecine 2, 39 Rue Jean Giroux, 34080 Montpellier (France); Brunet, Alexandre, E-mail: brunet.alexandre@outlook.com [CIRAD/US 49 Analyse, Avenue Agropolis, TA B-49/01, 34398 Montpellier Cedex (France); Babre, Daniel, E-mail: Daniel.babre@cirad.fr [CIRAD/US 49 Analyse, Avenue Agropolis, TA B-49/01, 34398 Montpellier Cedex (France); Alary, Karine, E-mail: Karine.alary@cirad.fr [CIRAD/US 49 Analyse, Avenue Agropolis, TA B-49/01, 34398 Montpellier Cedex (France); Sarthou, Jean-Pierre, E-mail: sarthou@ensat.fr [ENSAT/INRA/INP UMR AGIR. BP 52627, Chemin de Borde Rouge, 31326 Castanet-Tolosan Cedex (France); Charpentier, Hubert, E-mail: Charpentier.hub@wanadoo.fr [La Boisfarderie, Brives 36100 (France); Durand, Michel, E-mail: earldeslacs@orange.fr [Le Cazals, Castanet 81 150 (France); Benada, Jaroslav, E-mail: benada@vukrom.cz [Agrotest fyto, Kromeriz Institute, Havlíckova 2787, 76701 Kromeriz (Czech Republic); Henry, Marc, E-mail: henry@unistra.fr [UMR CNRS/UdS 7140, Université de Strasbourg, Institut Le Bel, 4, rue Blaise Pascal, CS 90032, Strasbourg 67081 (France)

    2016-02-04

    The soil redox potential (Eh) can provide essential information to characterise soil conditions. In practice, however, numerous problems may arise regarding: (i) Eh determination in soils, especially aerobic soils, e.g. variations in the instrumentation and methodology for Eh measurement, high spatial and temporal Eh variability in soils, irreversibility of the redox reaction at the surface electrode, chemical disequilibrium; and (ii) measurement interpretation. This study aimed at developing a standardised method for redox potential measurement in soils, in order to use Eh as a soil quality indicator. This paper presents practical improvements in soil Eh measurement, especially regarding the control of electromagnetic perturbations, electrode choice and preparation, soil sample preparation (drying procedure) and soil:water extraction rate. The repeatability and reproducibility of the measurement method developed are highlighted. The use of Eh corrected at pH7, pe+pH or rH{sub 2}, which are equivalent notions, is proposed to facilitate interpretation of the results. The application of this Eh measurement method allows characterisation of soil conditions with sufficient repeatability, reproducibility and accuracy to demonstrate that conservation agriculture systems positively alter the protonic and electronic balance of soil as compared to conventional systems. - Highlights: • Electromagnetic fields can dramatically perturb soil Eh measurement. • Our method overcomes the main difficulties in soil Eh measurement. • Accurate and reproducible measurement of mean soil Eh are achieved. • Eh{sub pH7}, pe+pH and rH{sub 2} are equivalent notions characterising electron activity. • Agricultural practices alter soil protonic and electronic characteristics.

  5. Genomic analyses provide insights into the history of tomato breeding.

    Science.gov (United States)

    Lin, Tao; Zhu, Guangtao; Zhang, Junhong; Xu, Xiangyang; Yu, Qinghui; Zheng, Zheng; Zhang, Zhonghua; Lun, Yaoyao; Li, Shuai; Wang, Xiaoxuan; Huang, Zejun; Li, Junming; Zhang, Chunzhi; Wang, Taotao; Zhang, Yuyang; Wang, Aoxue; Zhang, Yancong; Lin, Kui; Li, Chuanyou; Xiong, Guosheng; Xue, Yongbiao; Mazzucato, Andrea; Causse, Mathilde; Fei, Zhangjun; Giovannoni, James J; Chetelat, Roger T; Zamir, Dani; Städler, Thomas; Li, Jingfu; Ye, Zhibiao; Du, Yongchen; Huang, Sanwen

    2014-11-01

    The histories of crop domestication and breeding are recorded in genomes. Although tomato is a model species for plant biology and breeding, the nature of human selection that altered its genome remains largely unknown. Here we report a comprehensive analysis of tomato evolution based on the genome sequences of 360 accessions. We provide evidence that domestication and improvement focused on two independent sets of quantitative trait loci (QTLs), resulting in modern tomato fruit ∼100 times larger than its ancestor. Furthermore, we discovered a major genomic signature for modern processing tomatoes, identified the causative variants that confer pink fruit color and precisely visualized the linkage drag associated with wild introgressions. This study outlines the accomplishments as well as the costs of historical selection and provides molecular insights toward further improvement.

  6. Endophytic Bacteria as Biocontrol Agents of Tomato Bacterial Wilt Disease

    Directory of Open Access Journals (Sweden)

    Arika Purnawati

    2014-01-01

    Full Text Available Ralstonia solanacearum is a tomato pathogen causing wilt disease which can lose crop production up to 100%. This problem also has economical impact to Indonesia, though controlling this pathogen has been donebefore. Biological control using endophytic bacteria is one of alternative control methods to support agriculture sustainability. The objective of these experiments are isolating endophytic bacteria taken from healthy tomato stems and root as well as investigating patogens as biocontrol agents of tomato bacterial wilt disease. Among 10 isolates found, only Ps1 and Ps8 can inhibit R. solanacearum based on antagonistic test in vitro using seed coat method and 4-7 mm inhibition zone forming. In vivo test was applied in green house in 30 days old of tomatoes usingendophytic bacteria by theroot soak technique. The result shows Ps1 and Ps8 within 15-16 days incubation period have suppressed 8.07%-9.19% pathogen attack.

  7. The history of tomato: from domestication to biopharming.

    Science.gov (United States)

    Bergougnoux, Véronique

    2014-01-01

    Imported from the Andean region to Europe in the 16th century, today tomato is widespread throughout the world and represents the most economically important vegetable crop worldwide. Tomato is not only traded in the fresh market but is also used in the processing industry in soups, as paste, concentrate, juice, and ketchup. It is an incredible source of important nutrients such as lycopene, β-carotene and vitamin C, which all have positive impacts on human health. Its production and consumption is increasing with population growth. In this review, we report how tomato was already domesticated by the ancient Incan and Aztec civilizations, and how it came to Europe, where its breeding history started. The development of genetic, molecular biology and plant biotechnology have opened the doors towards the modern genetic engineering of tomato. The different goals of tomato genetic engineering are presented, as well as examples of successfully engineered tomatoes in terms of resistance to biotic and abiotic stresses, and fruit quality. The development of GM tomato for biopharming is also described.

  8. Validation of Growth and Nutrient Uptake Models for Tomato on a Gravelly South Florida Soil Under Greenhouse Conditions

    Institute of Scientific and Technical Information of China (English)

    D.A.CHIN; X.H.FAN; Y.C.LI

    2011-01-01

    The Soil and Water Assessment Tool (SWAT) has been widely used throughout the world to model crop growth and nutrient uptake in various types of soils. A greenhouse experiment was performed to validate the process equations embedded in SWAT for describing the growth and nutrient uptake of tomatoes in south Florida. The scaled growth curve of greenhouse-grown tomatoes was in close agreement with the theoretical model for field conditions, with the scaling factors being the maximum canopy height and the potential heat units. Similarly, the scaled leaf area index (LAI) growth curve and the scaled root depth curve for greenhousegrown tomatoes agreed with the SWAT functions, with the scaling factors being the maximum LAI and maximum root depth. The greenhouse experiment confirmed that the growth of biomass is a linear function of the intercepted photosynthetically active radiation.The fractions of nutrients in the plant biomass under greenhouse conditions were found to be on the order of 60% of those fractions observed in the field. Values of the initial P distribution (0.2 mg kg-1), initial ratio of mineral stable P to mineral active P (50:1),and initial ratio of humic N to humic P (2.4:1) were determined from soil measurements and can be used for field simulations. The conventional saturation-excess model for soil-water percolation was used to predict the movement of water in the top 10 cm of the greenhouse containers and the results agreed well with measurements.

  9. Seasonal trends of whitefly populations in a Mediterranean tomato growing area.

    Science.gov (United States)

    Nannini, M; Foddi, F; Murgia, G; Pisci, R; Sanna, F; Testa, M

    2009-01-01

    The whiteflies Bemisia tabaci and Trialeurodes vaporariorum and their associated viruses constitute a major threat to tomato crops in the Mediterranean region. Continuous host availability and mild climate are thought to be among the factors contributing to the outbreaks of whitefly-related problems in this area. We carried out a year-long survey to investigate the relative contribution of different plants, agricultural and not, and indoor/outdoor crops as hosts of the two whiteflies and the tomato yellow leaf curl disease (TYLCD) in a multi-crop system typical of tomato growing areas in southern Sardinia (S. Margherita di Pula, Cagliari, Italy). For this purpose, during 2005 we monitored whitefly population trends in different plots of a horticulture farm, evaluated seasonal changes in the infestation density of the two pests on the most represented host species and assessed the incidence of TYLCD on tomato crops and susceptible weeds. Whitefly catches on yellow sticky traps were found to be higher inside et along the external perimeter of greenhouses compared to open field crops or uncultivated areas, thus suggesting significant adult movement between indoor and outdoor patches. In most plots flight activity increased between late spring and late summer, peaking in July. The number of immatures of the two whitefly species showed similar dynamics, but while T. vaporariorum reached the highest densities in greenhouse tomato crops in June, peak levels of 8. tabaci were recorded between July and August in outdoor horticultural crops and weeds. The occurrence of TYLCD was detected all year round on weed hosts, but the highest number of infected plants was observed in June on long cycle tomato crops. The present survey has demonstrated the contribution of non-agricultural plants the maintenance of tomato yellow leaf curl disease in the study site. However, tomato crops established in summer as major reservoirs of TYLCD-associated viruses and presumably played a key

  10. A New Pest in Tomato Production: Lasioptera sp (Diptera:Cecidomyiidae

    Directory of Open Access Journals (Sweden)

    Nurdan Topakcı

    2016-11-01

    Full Text Available Tomato is one of the most important vegetable crop which is widely grown in the world. Economic yield loses due to several insect species has been reported on tomato production. In recent years, a new pest Lasioptera sp, which causes significant damage in tomato production area has emerged. The pest has first been reported in tomato production areas of Mersin district in 2012 after it has been reported in Greece. This pest belongs to the family Cecidomyiidae and is causing damage in the stems and on the fruits of tomato. The larva feeds inside of the stem, so this makes management of the pest difficult. There are no adequate studies on integrated pest management on this pest. This report clearly showed that detailed studies on the biology and management of this pest in the tomato production should be done.

  11. A field-grown transgenic tomato line expressing higher levels of polyamines reveals legume cover crop mulch-specific perturbations in fruit phenotype at the levels of metabolite profiles, gene expression, and agronomic characteristics

    OpenAIRE

    2008-01-01

    Genetic modification of crop plants to introduce desirable traits such as nutritional enhancement, disease and pest resistance, and enhanced crop productivity is increasingly seen as a promising technology for sustainable agriculture and boosting food production in the world. Independently, cultural practices that utilize alternative agriculture strategies including organic cultivation subscribe to sustainable agriculture by limiting chemical usage and reduced tillage. How the two together af...

  12. Resistance in tomato and wild relatives to crown and root rot caused by Phytophthora capsici.

    Science.gov (United States)

    Quesada-Ocampo, L M; Hausbeck, M K

    2010-06-01

    Phytophthora capsici causes root, crown, and fruit rot of tomato, a major vegetable crop grown worldwide. The objective of this study was to screen tomato cultivars and wild relatives of tomato for resistance to P. capsici. Four P. capsici isolates were individually used to inoculate 6-week-old seedlings (1 g of P. capsici-infested millet seed per 10 g of soilless medium) of 42 tomato cultivars and wild relatives of tomato in a greenhouse. Plants were evaluated daily for wilting and death. All P. capsici isolates tested caused disease in seedlings but some isolates were more pathogenic than others. A wild relative of cultivated tomato, Solanum habrochaites accession LA407, was resistant to all P. capsici isolates tested. Moderate resistance to all isolates was identified in the host genotypes Ha7998, Fla7600, Jolly Elf, and Talladega. P. capsici was frequently recovered from root and crown tissue of symptomatic inoculated seedlings but not from leaf tissue or asymptomatic or control plants. The phenotype of the recovered isolate matched the phenotype of the inoculum. Pathogen presence was confirmed in resistant and moderately resistant tomato genotypes by species-specific polymerase chain reaction of DNA from infected crown and root tissue. Amplified fragment length polymorphisms of tomato genotypes showed a lack of correlation between genetic clusters and susceptibility to P. capsici, indicating that resistance is distributed in several tomato lineages. The results of this study create a baseline for future development of tomato cultivars resistant to P. capsici.

  13. A rapid seedling resistance assay identifies wild tomato lines that are resistant to Psuedomonas syringe pv. tomato race 1

    Science.gov (United States)

    Bacterial speck caused by Pseudomonas syringae has historically been controlled by the Pto/Prf gene cluster. Emerging strains like P. syringae pv. tomato race 1 overcome resistance conferred by Pto/Prf, and can cause serious crop loss under appropriate environmental conditions. We developed a rapid ...

  14. Evidence of ozone-induced adverse effects on crops in the Mediterranean region

    Science.gov (United States)

    Fumagalli, Ivano; Gimeno, Benjamin S.; Velissariou, Dimitris; De Temmerman, Ludwig; Mills, Gina

    The impacts of ambient ozone pollution on crops in the Mediterranean countries have been recorded regularly in the so-called "grey literature" of UN/ECE Workshop Reports for the Convention on Long-range Transboundary Air Pollution, and less frequently in the peer-reviewed literature. This short communication reviews such records and shows that ambient ozone episodes have been reported to cause visible injury on 24 agricultural and horticultural crops grown in commercial fields including three of the most important crops in the region (wheat, maize, and grapevine). On one occasion, the damage was so extensive that complete crop loss occurred in commercial glasshouses of Butterhead lettuce in one area of Greece. Experiments with open-top chambers have indicated that ambient ozone caused 17-39% yield loss in crops such as wheat, bean, watermelon and tomato. The applicability of the long-term critical level of ozone described by Fuhrer et al. (Environ. Pollut. 97 (1997) 91) for the Mediterranean areas is also considered.

  15. Avaliações qualitativas e quantitativas de plantas daninhas na cultura da soja submetida aos sistemas de plantio direto e convencional Quantitative and qualitative weed evaluation of soybean crop in no-tillage and conventional tillage systems

    Directory of Open Access Journals (Sweden)

    Eduardo de Sá Pereira

    2000-08-01

    pré-emergência proporcionaram melhor controle de A. viridis do que de B. plantaginea; o controle com herbicidas pós-emergentes foi insatisfatório para ambas espécies. No plantio direto, o controle de E. heterophylla foi insatisfatório em todos os sistemas de controle testados. O plantio direto apresentou sempre menor número total de plantas daninhas, sobretudo de gramineas. A germinação de plantas daninhas limitou-se ao período de até 15 dias após a emergência da cultura, nos dois sistemas de cultivo.With the objective to evaluate the effects of different tillage systems and different methods of control on mulch of black oat (Avena strigosa on evolution and control of weeds in soybean crop (cultivar 'IAC 14', a field experiment was carried out in Fazenda Experimental Lageado - UNESP Botucatu - SP, in 1993/94. The different cultivation systems were no tillage and conventional tillage, and the last cultivation system was made with disk harrow and three tandem disk harrow. The weed control methods were: control (weedy check, pre-emergence herbicides (0,28 kg/ha of metribuzim + 1,29 kg/ha of oryzalin, post-emergence herbicides (0,25 kg/ha of Fluacifop-p-butil +0,25 kg/ha of fomesafen and pre and post-emergence treatments (with the four herbicides in the same rates. Glyphosate was applied for weed and black oat elimination before the crop establishment. The experimental design used was a randomized block arranged in split-plots scheme with four replications. The different cultivation systems were applied on plots and the different weed control on sub-plots. In the early stages of crop development, the rain was scarce, limiting crop growth and performance of the preemergence herbicides. The number the weeds/m2 was evaluated at 14, 28 and 35 days after soybean emergency, showing differences between cultivation systems and among different control systems. Brachiaria plantaginea and Amaranthus viridis were predominant on conventional tillage, and less important

  16. High-efficient cultivation techniques of tomato in greenhouse of south China during winter-spring cropping season%南方设施大棚番茄冬春大茬高效栽培技术

    Institute of Scientific and Technical Information of China (English)

    陈华; 薛珠政

    2012-01-01

    The growth period of winter-spring tomato in greenhouse at the coastal areas of Fujian Province is around 8-months and its income is 20 000-25 000 yuan per 667m2.This paper summarized cultivation techniques of winter-spring tomato in greenhouse,including selection of cultivars,sowing and seedlings,management of water and fertilization,pruning,hanging vine,preserving suitable numbers of flowers and fruits,management of temperature and humidity,control of diseases and pests,and harvest in due time.%福建省沿海地区设施大棚番茄冬春大茬生产的生产期有8个月左右,每667 m2收入2.0万~2.5万元。该文从品种选择、播种育苗、整地定植、水肥管理、整枝打叶、吊蔓与落蔓、保花保果疏果、大棚温湿度管理、病虫害防治、适时采收等几个方面总结了南方设施大棚番茄冬春大茬高效栽培技术。

  17. Crop protection in organic agriculture

    NARCIS (Netherlands)

    Letourneau, D.; Bruggen, van A.H.C.

    2006-01-01

    The authors describe pests and diseases and their management in organic versus conventional agriculture. Also two case studies are described: 1. Pest and pathogen regulation in organic versus conventional cereal crops in Europe and 2. Pest and pathogen regulation in organic versus conventional tomat

  18. Soil Chemical Characteristics of Organic and Conventional Agriculture

    Directory of Open Access Journals (Sweden)

    Muhammad Abdul Aziz

    2016-01-01

    Full Text Available Use of chemical fertilizers and pesticides on intensive land of both lowland and upland food crops have been shown to increase agricultural productivity significantly. Research aimed to study soil chemical characteristics and soil pesticide residues at some crops of organic and conventional farms. The research was carried out in Laboratory of Soil Chemistry, Indonesian Soil Research Institute and in Laboratory of Agrochemical Residue, Indonesian Agricultural Environment Research Institute, Bogor from February to July 2015. Soil samples at 0-10 cm depth were taken compositely from broccoli (Brassica oleracea, carrots (Daucus carota, maize (Zea mays, and tomatoes (Solanum lycopersicum farms in Bogor Regency as well as from rice field in Tasikmalaya Regency at both organic and conventional farms. Soil chemical characteristics were analyzed include: soil organic-C (Walkey and Black, total-N (Kjeldahl, potential-P (HCl 25%, available-P (Olsen, potential-K (HCl 25%, available-K (NH4OAc 1 N pH 7, CEC (NH4OAc 1 N pH 7, and pH (soil : water = 1: 5, while pesticide residues included levels of organochlorine (lindane, aldrin, heptaklor, dieldrin, DDT, endosulfan; organophosphates (diazinon, fenitrotin, metidation, paration, profenofos; and carbamates (carbofuran, MIPC, BPMC in the soil by using Gas Chromatography method. Results showed that levels of soil organic-C, total-N, potential and available-P, potential and available-K, CEC, pH at organic farms were higher than those at conventional farms. Some pesticide residues compound (organochlorines, organophosphates, and carbamates were detected at conventional farm, while those at organic farm were not detected (trace.

  19. Análise de correspondência como instrumento para descrição do perfil do trabalhador da cultura de tomate de mesa em Goiás The correspondence analysis as instrument to analyze the profile of the worker in the tomato crop in the Goiás state, Brazil

    Directory of Open Access Journals (Sweden)

    Sueli Martins Freitas Alves

    2009-10-01

    Full Text Available O cultivo do tomate de mesa em Goiás está estruturado numa proposta tecnológica baseada no uso intensivo de agrotóxicos, o que envolve riscos e danos potenciais à saúde dos trabalhadores. O objetivo deste trabalho foi utilizar a Análise de Correspondência Múltipla (ACM para explorar a perfil do trabalhador da cultura de tomate de mesa quanto ao uso de agrotóxicos. Para isso, foram obtidas informações sobre fatores socioeconômicos e demográficos, percepção de risco, uso de agrotóxicos, uso de equipamento de proteção individual (EPI e morbidade referida, por meio de questionário respondido por 96 trabalhadores da cultura de tomate de mesa, em seis municípios do Estado de Goiás. A Análise de Correspondência Múltipla (ACM possibilitou definir três grupos de trabalhadores. No grupo 1, os que responderam que já se intoxicaram com agrotóxicos e não usam EPI porque dificulta o trabalho ou porque não têm costume. No grupo 2, os que responderam que não se intoxicaram com agrotóxico relataram que usam somente algum tipo de EPI porque os consideram desconfortáveis ou por outros motivos. No grupo 3, os que relataram usar EPI completo e que moram em barracas de lona na lavoura de tomate. Foi possível fazer correspondência entre as modalidades da pesquisa, o que não seria possível com o uso de estatística descritiva.The tomato cropping in the Goiás state is structured under a technological proposal based on the intensive use of pesticides, which involves risks and potential damages to the workers' health. This study aimed to use the Multiple Correspondence Analysis (ACM to explore the worker's profile concerning to the use of pesticides in tomato crop. So, information about the socioeconomic and demographic factors, risk perception, pesticide uses, use of individual protection equipment (EPI and referred morbidity were obtained, by using a questionnaire applied to 96 workers in tomato crop, in six counties at the State

  20. Agronomic efficiency of intercropping tomato and lettuce

    Directory of Open Access Journals (Sweden)

    Arthur B. Cecílio Filho

    2011-09-01

    Full Text Available Four experiments were carried out at the São Paulo State University, Brazil, with the aim of determining the agronomic viability of intercropping tomato and lettuce, under greenhouse conditions. The studied intercropping systems were established by transplanting lettuce at 0, 10, 20 and 30 days after transplanting (DAT tomato and by transplanting tomato at 0, 10, 20 and 30 DAT lettuce. Intercropped tomato and lettuce were evaluated during two seasons and compared to their sole cropping. The experimental design was a randomized complete block with nine treatments. The productivity and the classification of the tomato fruits were not influenced by having lettuce intercropped with it, but lettuce production was lowered when tomato was intercropped with it. The longer the delay in lettuce transplanting, the greater the reduction in its productivity. There was an effect of cropping season on the extent of the agronomic advantage of intercropping over sole cropping. In the first cropping season, intercropping established by transplanting lettuce during the interval between 30 days before up to 20 DAT tomato yielded land use efficiency (LUE indices of 1.63 to 2.22. In the second period, intercropping established with the transplanting of lettuce up to 30 days before tomato yielded LUE indices of 1.57 to 2.05.Quatro experimentos foram conduzidos na Unesp, Brasil, com o objetivo de determinar a viabilidade agronômica de cultivos consorciados de alface e tomate em ambiente protegido. Consórcios estabelecidos por transplantes da alface aos 0, 10, 20 e 30 dias após o transplante (DAT do tomate e de tomate aos 0, 10, 20 e 30 DAT da alface, foram avaliados em duas épocas e comparados às suas monoculturas. Cada experimento foi conduzido em delineamento de blocos ao acaso, com nove tratamentos. Verificou-se que a produtividade do tomate e a classificação dos frutos não foram influenciadas pela alface, mas a produção da alface foi menor em cons

  1. Gibberellic acid contribution to tomato fruit size

    Directory of Open Access Journals (Sweden)

    Ricardo Antonio Ayub

    2010-12-01

    Full Text Available With the aim of assessing the behavior of tomato fruits subjected to increasing concentrations of gibberellic acid (GA3, research was carried out in the municipality of Irati, Paraná State, Brazil, within a commercial area of tomato crops, cultivar Fanny, in September under the crossing fence system with a single branch per plant. The adopted treatments were as follows: 0, 30, 60, 90, and 120ppm of GA3, applied whenever the diameter of the fi rst fruits of the second clusters reached roughly 10 mm. At harvest when the fruits had achieved 30 to 50% of reddish color, the fresh mass, length, and longitudinal and transversal diameters were measured. The application of 120ppm of GA3 provided maximum fresh mass and dimensions.

  2. Biomassa e atividade microbiana do solo em sistemas de produção olerícola orgânica e convencional Biomass and soil microbial activity on horticulture organic and conventional crop systems

    Directory of Open Access Journals (Sweden)

    Rogério Otávio Schmidt

    2013-02-01

    Full Text Available O cultivo de olerícolas pode ser produzido no sistema convencional utilizando defensivos agrícolas e fertilizantes sintéticos, ou no sistema orgânico, quando não há a utilização de defensivos sintéticos e tem como principal fonte de adubação, resíduos orgânicos. Este trabalho teve como objetivo avaliar a biomassa e a atividade microbiana do solo em sistemas de cultivo orgânico e convencional com suas respectivas áreas de referência. Para isso, foram coletadas amostras de solo em três propriedades agrícolas com sistema de cultivo convencional e outras três com sistema de cultivo orgânico, além de suas respectivas áreas de referência. Foram determinados o carbono e nitrogênio microbiano e total, e a respiração basal após 57 dias de incubação. A maior variação na taxa de respiração basal entre a área de produção e a área de referência foi observada no sistema orgânico. Os teores de nutrientes no solo, carbono e nitrogênio microbianos foram maiores nas áreas de produção do que os encontrados nas áreas de referência.The horticultural crops can be produced with the conventional system using pesticides and synthetic fertilizers, or organic system where there is no use of synthetic pesticides and its main source of nutrients are organic wastes. This study aimed to assess the biomass and soil microbial activity in organic production systems and conventional with their respective reference areas. In this study, soil samples were sampled in three farms with conventional tillage and three other with organ system, and their respective reference areas. It was determined total carbon and nitrogen and microbial, and microbial activity after 57 days of incubation. The greatest variation in basal respiration rate between the respective production area and reference area was observed in the organic system. The nutrients in the soil microbial carbon and nitrogen were higher in the production areas than those in the

  3. Guidelines to use tomato in experiments with a controlled environment.

    Directory of Open Access Journals (Sweden)

    Dietmar eSchwarz

    2014-11-01

    Full Text Available Domesticated tomato (Solanum lycopersicum is the most important horticultural crop worldwide. Low polymorphism at the DNA level conflicts with the wealth of morphological variation. Fruits vary widely in size, shape and colour. In contrast, genetic variation between the 16 wild relatives is tremendous. Several large seed banks provide tomato germplasm for both domesticated and wild accessions of tomato. Recently, the genomes of the inbred cultivar Heinz 1706 (≈900 Mb and S. pimpinellifolium (739 Mb were sequenced. Genomic markers and genome re-sequencing data are available for >150 cultivars and accessions. Transformation of tomato is relatively easy and T-DNA insertion line collections are available. Tomato is widely used as a model crop for fruit development but also for diverse physiological, cellular, biochemical, molecular and genetic studies. It can be easily grown in greenhouses or growth chambers. Plants grow, flower, and develop fruits well at daily light lengths between 8-16 hours. The required daily light integral of an experiment depends on growth stage and temperature investigated. Temperature must be 10-35°C, relative humidity 30-90 % and CO2 concentration 200-1500 µmol mol-1. Temperature determines the speed of the phenological development while daily light integral and CO2 concentration affect photosynthesis and biomass production. Seed to seed cultivation takes 100 days at 20°C and can be shortened or delayed by temperature. Tomato may be cultivated in soil, substrates, or aeroponically without any substrate. Root volume and water uptake requirements are primarily determined by transpiration demands of the plants. Many nutrient supply recipes and strategies are available to ensure sufficient supply as well as specific nutrient deficits/surplus. Using appropriate cultivation techniques makes tomato a convenient model plant for researchers, even for beginners.

  4. Guidelines to use tomato in experiments with a controlled environment.

    Science.gov (United States)

    Schwarz, Dietmar; Thompson, Andrew J; Kläring, Hans-Peter

    2014-01-01

    Domesticated tomato (Solanum lycopersicum) is the most important horticultural crop worldwide. Low polymorphism at the DNA level conflicts with the wealth of morphological variation. Fruits vary widely in size, shape, and color. In contrast, genetic variation between the 16 wild relatives is tremendous. Several large seed banks provide tomato germplasm for both domesticated and wild accessions of tomato. Recently, the genomes of the inbred cultivar "Heinz 1706" (≈900 Mb), and S. pimpinellifolium (739 Mb) were sequenced. Genomic markers and genome re-sequencing data are available for >150 cultivars and accessions. Transformation of tomato is relatively easy and T-DNA insertion line collections are available. Tomato is widely used as a model crop for fruit development but also for diverse physiological, cellular, biochemical, molecular, and genetic studies. It can be easily grown in greenhouses or growth chambers. Plants grow, flower, and develop fruits well at daily light lengths between 8 and 16 h. The required daily light integral of an experiment depends on growth stage and temperature investigated. Temperature must be 10-35°C, relative humidity 30-90%, and, CO2 concentration 200-1500 μmol mol(-1). Temperature determines the speed of the phenological development while daily light integral and CO2 concentration affect photosynthesis and biomass production. Seed to seed cultivation takes 100 days at 20°C and can be shortened or delayed by temperature. Tomato may be cultivated in soil, substrates, or aeroponically without any substrate. Root volume, and water uptake requirements are primarily determined by transpiration demands of the plants. Many nutrient supply recipes and strategies are available to ensure sufficient supply as well as specific nutrient deficits/surplus. Using appropriate cultivation techniques makes tomato a convenient model plant for researchers, even for beginners.

  5. Guidelines to use tomato in experiments with a controlled environment

    Science.gov (United States)

    Schwarz, Dietmar; Thompson, Andrew J.; Kläring, Hans-Peter

    2014-01-01

    Domesticated tomato (Solanum lycopersicum) is the most important horticultural crop worldwide. Low polymorphism at the DNA level conflicts with the wealth of morphological variation. Fruits vary widely in size, shape, and color. In contrast, genetic variation between the 16 wild relatives is tremendous. Several large seed banks provide tomato germplasm for both domesticated and wild accessions of tomato. Recently, the genomes of the inbred cultivar “Heinz 1706” (≈900 Mb), and S. pimpinellifolium (739 Mb) were sequenced. Genomic markers and genome re-sequencing data are available for >150 cultivars and accessions. Transformation of tomato is relatively easy and T-DNA insertion line collections are available. Tomato is widely used as a model crop for fruit development but also for diverse physiological, cellular, biochemical, molecular, and genetic studies. It can be easily grown in greenhouses or growth chambers. Plants grow, flower, and develop fruits well at daily light lengths between 8 and 16 h. The required daily light integral of an experiment depends on growth stage and temperature investigated. Temperature must be 10–35°C, relative humidity 30–90%, and, CO2 concentration 200–1500 μmol mol−1. Temperature determines the speed of the phenological development while daily light integral and CO2 concentration affect photosynthesis and biomass production. Seed to seed cultivation takes 100 days at 20°C and can be shortened or delayed by temperature. Tomato may be cultivated in soil, substrates, or aeroponically without any substrate. Root volume, and water uptake requirements are primarily determined by transpiration demands of the plants. Many nutrient supply recipes and strategies are available to ensure sufficient supply as well as specific nutrient deficits/surplus. Using appropriate cultivation techniques makes tomato a convenient model plant for researchers, even for beginners. PMID:25477888

  6. Predictive modeling of infrared radiative heating in tomato dry-peeling process: Part I. Model development

    Science.gov (United States)

    Infrared (IR) dry-peeling has emerged as an effective non-chemical alternative to conventional lye and steam methods of peeling tomatoes. Successful peel separation induced by IR radiation requires the delivery of a sufficient amount of thermal energy onto tomato surface in a very short duration. Th...

  7. PRODUCTION OF TOMATO SEEDLINGS UNDER SALINE IRRIGATION

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Brasiliano Campos

    2007-01-01

    Full Text Available Processing tomato is the most important vegetable crop of the Brazilian agribusiness and few researches have been conducted to evaluate the tolerance of this crop to saline stress. In this study, the effects of five levels of salinity of the irrigation water (1, 2, 3, 4 and 5 dS m-1 and three equivalent proportions of Na:Ca:Mg (1:1:0.5, 4:1:0.5 and 7:1:0.5 were tested on the emergence and vigor of processing tomato, cultivar IPA 6. Seeds were sowed in expanded polystyrene tray (128 cells and each tray received 1 L of water after sowing. The trays were piled and, four days after sowing, they were placed on suspended supports in a greenhouse. Irrigation was accomplished daily from the fifth day after sowing. Only dry weight of shoot and root was affected by sodium proportions, while linear reductions of the speed of emergence, stem length and the dry weight of shoot and root were observed with increasing salinity. Root was more affected than shoot by salinity and relative growth ratioincreased with salinity levels on the 14-21 days after sowing period, indicating that the crop showed a certain increase of salinity tolerance with the time of exposure to salts.

  8. Adubação nitrogenada no feijoeiro em sistema de semeadura direta e preparo convencional do solo = Nitrogen fertilization in common bean crops under no-tillage and conventional systems

    Directory of Open Access Journals (Sweden)

    Francisco Guilhien Gomes Junior

    2008-12-01

    . The experiment was conducted over two years (2002 and 2003 in no-tillage onmillet crop residues and conventional plow system. It was concluded that N fertilizer at the V4 stadium of common bean promotes similar seed yields in no-tillage and conventional systems. Yield differences between no-tillage and conventional systems are inconsistent inthe same agricultural area.

  9. Tomato seeds for LDEF

    Science.gov (United States)

    1983-01-01

    Tomato seeds are prepared for their launch aboard the Langley's Long Duration Exposure Facility. Photograph published in Winds of Change, 75th Anniversary NASA publication (page 119), by James Schultz.

  10. Effects of Continuous Tomato Monoculture on Soil Microbial Properties and Enzyme Activities in a Solar Greenhouse

    Directory of Open Access Journals (Sweden)

    Hongdan Fu

    2017-02-01

    Full Text Available Soil-related obstacles resulting from continuous monoculture have limited the sustainable development of the tomato industry in China. An experiment on tomatoes with seven continuous monoculture treatments (the 1st, 3rd, 5th, 7th, 9th, 11th, and 13th crops, respectively was conducted in a solar greenhouse, to investigate the influence of monoculture on soil quality. Most soil quality indicators first increased and then decreased with increasing continuous monoculture crops, and significant differences among crops were observed. Indicators at the 13th crop were significantly lower than those at the other crops in terms of average well color development (AWCD, substrate richness (S, the Shannon diversity index (H, and the McIntosh index (U of the soil microbial community (SMC, soil urease (UR, and neutral phosphatase (N-PHO activities, and available nitrogen (AN and potassium (AK. However, fungal abundance (FUN at the 13th crop was significantly higher than that at the other crops. As principal component analysis (PCA revealed, SMC functional diversity at the 1st, 11th, and 13th crops were similar, and were obviously distinguished from those at the other crops. Moreover, the tomato yield was significantly and positively correlated with soil-available potassium and SMC functional diversity indexes. Our findings indicated that short-term continuous monoculture, e.g., for fewer than seven or nine crops, was beneficial for soil quality improvement. However, continuous monoculture for greater than 11 crops had adverse effects on soil enzyme activities, soil microbial abundances, soil chemical properties, soil SMC functional diversity, and the tomato yield, particularly at the 13th crop.

  11. The role of specific tomato volatiles in tomato-whitefly interaction.

    Science.gov (United States)

    Bleeker, Petra M; Diergaarde, Paul J; Ament, Kai; Guerra, José; Weidner, Monique; Schütz, Stefan; de Both, Michiel T J; Haring, Michel A; Schuurink, Robert C

    2009-10-01

    Bemisia tabaci (whitefly) infestations and the subsequent transfer of viruses are the cause of severe losses in crop production and horticultural practice. To improve biological control of B. tabaci, we investigated repellent properties of plant-produced semiochemicals. The mix of headspace volatiles, collected from naturally repellent wild tomato accessions, influenced B. tabaci initial choice behavior, indicating a role for plant semiochemicals in locating host plants. A collection of wild tomato accessions and introgression lines (Solanum pennellii LA716 x Solanum lycopersicum 'Moneyberg') were extensively screened for attractiveness to B. tabaci, and their headspace profiles were determined by means of gas chromatography-mass spectrometry. Correlation analysis revealed that several terpenoids were putatively involved in tomato-whitefly interactions. Several of these candidate compounds conferred repellence to otherwise attractive tomato plants when applied to the plant's branches on paper cards. The sesquiterpenes zingiberene and curcumene and the monoterpenes p-cymene, alpha-terpinene, and alpha-phellandrene had the strongest effects in free-choice bioassays. These terpenes also elicited a response of receptors on the insect's antennae as determined by electroantennography. Conversely, the monoterpene beta-myrcene showed no activity in both assays. B. tabaci apparently uses, besides visual cues, specific plant volatile cues for the initial selection of a host. Altering whitefly choice behavior by manipulation of the terpenoid composition of the host headspace may therefore be feasible.

  12. The Role of Specific Tomato Volatiles in Tomato-Whitefly Interaction1[W][OA

    Science.gov (United States)

    Bleeker, Petra M.; Diergaarde, Paul J.; Ament, Kai; Guerra, José; Weidner, Monique; Schütz, Stefan; de Both, Michiel T.J.; Haring, Michel A.; Schuurink, Robert C.

    2009-01-01

    Bemisia tabaci (whitefly) infestations and the subsequent transfer of viruses are the cause of severe losses in crop production and horticultural practice. To improve biological control of B. tabaci, we investigated repellent properties of plant-produced semiochemicals. The mix of headspace volatiles, collected from naturally repellent wild tomato accessions, influenced B. tabaci initial choice behavior, indicating a role for plant semiochemicals in locating host plants. A collection of wild tomato accessions and introgression lines (Solanum pennellii LA716 × Solanum lycopersicum ‘Moneyberg’) were extensively screened for attractiveness to B. tabaci, and their headspace profiles were determined by means of gas chromatography-mass spectrometry. Correlation analysis revealed that several terpenoids were putatively involved in tomato-whitefly interactions. Several of these candidate compounds conferred repellence to otherwise attractive tomato plants when applied to the plant's branches on paper cards. The sesquiterpenes zingiberene and curcumene and the monoterpenes p-cymene, α-terpinene, and α-phellandrene had the strongest effects in free-choice bioassays. These terpenes also elicited a response of receptors on the insect's antennae as determined by electroantennography. Conversely, the monoterpene β-myrcene showed no activity in both assays. B. tabaci apparently uses, besides visual cues, specific plant volatile cues for the initial selection of a host. Altering whitefly choice behavior by manipulation of the terpenoid composition of the host headspace may therefore be feasible. PMID:19692533

  13. The intercropping partner affects arbuscular mycorrhizal fungi and Fusarium oxysporum f. sp. lycopersici interactions in tomato.

    Science.gov (United States)

    Hage-Ahmed, Karin; Krammer, Johannes; Steinkellner, Siegrid

    2013-10-01

    Arbuscular mycorrhizal fungi (AMF) and their bioprotective aspects are of great interest in the context of sustainable agriculture. Combining the benefits of AMF with the utilisation of plant species diversity shows great promise for the management of plant diseases in environmentally compatible agriculture. In the present study, AMF were tested against Fusarium oxysporum f. sp. lycopersici with tomato intercropped with either leek, cucumber, basil, fennel or tomato itself. Arbuscular mycorrhizal (AM) root colonisation of tomato was clearly affected by its intercropping partners. Tomato intercropped with leek showed even a 20 % higher AM colonisation rate than tomato intercropped with tomato. Positive effects of AMF expressed as an increase of tomato biomass compared to the untreated control treatment could be observed in root as well as in shoot weights. A compensation of negative effects of F. oxysporum f. sp. lycopersici on tomato biomass by AMF was observed in the tomato/leek combination. The intercropping partners leek, cucumber, basil and tomato had no effect on F. oxysporum f. sp. lycopersici disease incidence or disease severity indicating no allelopathic suppression; however, tomato co-cultivated with tomato clearly showed a negative effect on one plant/pot with regard to biomass and disease severity of F. oxysporum f. sp. lycopersici. Nonetheless, bioprotective effects of AMF resulting in the decrease of F. oxysporum f. sp. lycopersici disease severity were evident in treatments with AMF and F. oxysporum f. sp. lycopersici co-inoculation. However, these bioprotective effects depended on the intercropping partner since these effects were only observed in the tomato/leek and tomato/basil combination and for the better developed plant of tomato/tomato. In conclusion, the effects of the intercropping partner on AMF colonisation of tomato are of great interest for crop plant communities and for the influences on each other. The outcome of the bioprotective

  14. Detection and frequency of recombination in tomato-infecting begomoviruses of South and Southeast Asia

    OpenAIRE

    Rai Mathura; Prasanna HC

    2007-01-01

    Abstract Background Tomato-infecting begomoviruses are widely distributed across the world and cause diseases of high economic impact on wide range of agriculturally important crops. Though recombination plays a pivotal role in diversification and evolution of these viruses, it is currently unknown whether there are differences in the number and quality of recombination events amongst different tomato-infecting begomovirus species. To examine this we sought to characterize the recombination e...

  15. Developing an Integrated Pest Management Program for Tomatoes in The Red River Delta of Vietnam: A mini review

    Directory of Open Access Journals (Sweden)

    Tuan M. Ha

    2015-08-01

    Full Text Available Ecologically based approaches to pest management in crop production have been embraced in recent decades due to their validity and effectiveness. Integrated Pest Management (IPM is not a new concept. It has been adopted in various regions in Vietnam, particularly in tomato production, an economically important vegetable crop in the Red River Delta (RRD. Given the occurrence and development of tomato pests are influenced by many factors such as soil types, crop varieties and growth habits, production practices, local climatic conditions, and growing seasons, this paper therefore developed an IPM program for tomatoes in the RRD based on the defined major pests in the region. Detailed factsheets for six major pests were developed and different components of the IPM were explored and employed for the ease of identification and management. Practical suggestions for tomato growers were also presented.

  16. Dry matter production and nutrient accumulation after successive crops of lettuce, tomato, rice, and andropogongrass in a substrate with zeolite Produção de matéria seca e acúmulo de nutrientes após cultivos sucessivos com alface, tomate, arroz e capim andropogon em substrato com zeólita

    Directory of Open Access Journals (Sweden)

    Alberto C. de Campos Bernardi

    2010-04-01

    Full Text Available Zeolites are hydrated crystalline aluminosilicate minerals of natural occurrence, structured in rigid third dimension net that can be used as slow release plant-nutrient source. The main objective of this study was to evaluate the effects of plant growth substrate under zeolite application, enriched with N, P and K, on dry matter yield and on nutrient contents in consecutive crops of lettuce, tomato, rice, and andropogon grass. The experiment was carried out in a greenhouse, with 3 kg pots with an inert substrate, evaluated in a randomized block design with three replications. Treatments consisted of four types of enrichment of concentrated natural zeolite: concentrated zeolite (Z only, zeolite + KNO3 (ZNK, zeolite + K2HPO4 (ZPK and zeolite + H3PO4 + apatite (ZP, and a control grown in substrate fertilized with a zeolite-free nutrient solution. Four levels of enriched zeolite were tested: 20, 40, 80, and 160 g/pot. Four successive crops were grown on the same substrate in each pot: lettuce, tomato, rice, and andropogon grass. Results indicated that N, P and K enriched zeolite was an adequate slow-release nutrient source for plants. The total dry matter production of above-ground biomass of four successive crops followed a descending order: ZP > ZPK > ZNK > Z.Zeólitas são minerais aluminossilicatos cristalinos hidratados de ocorrência natural, estruturados em redes cristalinas tridimensionais rígidas que podem ser usados como fonte de liberação lenta de nutrientes para as plantas. O objetivo deste trabalho foi avaliar o efeito da adição de zeólita enriquecida com N, P e K ao substrato de cultivo sobre a produção de matéria seca e o acúmulo de nutrientes pelas culturas sucessivas de alface, de tomate, de arroz e de capim-andropogon. O experimento foi conduzido em vasos em casa de vegetação, em vasos contendo 3 kg de substrato inerte. O delineamento experimental foi o de blocos ao acaso, com três repetições. A zeólita natural

  17. Fontes de resistência em tomateiro aos begomovírus bissegmentados Tomato yellow spot virus e Tomato severe rugose virus Sources of resistance in tomato to bipartite begomoviruses Tomato yellow spot virus and Tomato severe rugose virus

    Directory of Open Access Journals (Sweden)

    Francisco D Hurtado

    2012-12-01

    Full Text Available Os begomovírus causam doenças de grande importância econômica em diversas culturas, principalmente em regiões tropicais e subtropicais. Juntamente com outras famílias de vírus, os begomovírus têm causado grande prejuízo para os produtores de tomate in natura e para processamento industrial. O objetivo deste trabalho foi avaliar o comportamento de 11 genótipos resistentes ao Tomato yellow leaf curl virus (TYLCV frente à infecção pelos begomovírus Tomato yellow spot virus (ToYSV e Tomato severe rugose virus (ToSRV em condições de casa-vegetação. A inoculação das plantas foi realizada via biobalística no estádio de duas folhas verdadeiras. A infecção viral confirmou-se pelo desenvolvimento dos sintomas e pela técnica de hibridização dot blot. Selecionaram-se como promissores os genótipos STY2, STY5, STY6 e L7, por não apresentarem sintomas e por terem concentrações virais muito baixas para os dois vírus. O espectro de resistência dos genes Ty-1 e Ty-2 não resultaram efetivos ante as espécies virais empregadas no estudo. As linhagens TY52, H24 e CLN2116B, portadoras destes genes, foram suscetíveis aos vírus ToYSV e ToSRV.Begomoviruses cause diseases of major economic importance in many crops, especially in tropical and subtropical regions. Together with other families of viruses, the begomoviruses cause great damage for producers of fresh and processed tomatoes. The objective of this study was to evaluate the behavior of 11 resistant genotypes to Tomato yellow leaf curl virus (TYLCV when infected by the begomoviruses Tomato severe rugose virus (ToSRV and Tomato yellow spot virus (ToYSV under greenhouse conditions. Tomato plants were inoculated by biobalistic method when displaying two true leaves. Viral infection was confirmed by visual observation of symptoms and by dot blot hybridization. Genotypes STY2, STY5, STY6 and L7 were selected as promising due to the absence of symptoms and very low viral

  18. Assessment of genetic diversity in tomato landraces using ISSR markers

    Directory of Open Access Journals (Sweden)

    Henareh Mashhid

    2016-01-01

    Full Text Available Tomato is one of the most economically important vegetable crops in many parts of the world. Turkey and Iran are the main producers of tomatoes in the world. The objective of this study was to assess the genetic variation of 93 tomato landraces from East Anatolian region of Turkey and North-West of Iran, along with three commercial cultivars using 14 ISSR primers. The percentage of polymorphic loci (PPL for all primers was 100%. The mean of expected heterozygosity (He for the primers varied from 0.153 (UBC808 to 0.30 (UBC848. The dendrogram placed the landraces and commercial cultivars into nine groups. The genotypes originating from the same region, often located in the same group or two adjacent groups. The highest likelihood of the data was obtained when population were located into 2 sub-populations (K = 2. These sub-populations had Fst value of 0.16 and 0.21.

  19. Heirloom tomato cultivars and local populations as sources of genetic variability for breeding

    Directory of Open Access Journals (Sweden)

    Glogovac Svetlana

    2010-01-01

    Full Text Available Five local tomato populations and fourteen heirloom cultivars were analyzed in this study. The analyzed genotypes represent a part of tomato collection of Institute of Field and Vegetable Crops in Novi Sad. The following fruit and plant traits were analyzed: growth type, fruit color, fruit shape index, fruit weight, number of locules and dry matter content. Cluster analysis was performed so as to group the analyzed genotypes by homology and divergence. The aim of this article was to determine the importance of heirloom cultivars and local populations as sources of genetic variability in tomato breeding process.

  20. Evolutionary analysis of tomato Sw-5 resistance-breaking isolates of Tomato spotted wilt virus.

    Science.gov (United States)

    López, Carmelo; Aramburu, José; Galipienso, Luis; Soler, Salvador; Nuez, Fernando; Rubio, Luis

    2011-01-01

    Tomato spotted wilt virus (TSWV) causes severe economic losses in many crops worldwide and often overcomes resistant cultivars used for disease control. Comparison of nucleotide and amino acid sequences suggested that tomato resistance conferred by the gene Sw-5 can be overcome by the amino acid substitution C to Y at position 118 (C118Y) or T120N in the TSWV movement protein, NSm. Phylogenetic analysis revealed that substitution C118Y has occurred independently three times in the studied isolates by convergent evolution, whereas the substitution T120N was a unique event. Analysis of rates of non-synonymous and synonymous changes at individual codons showed that substitution C118Y was positively selected.

  1. Molecular dissection of Tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum.

    Science.gov (United States)

    Anbinder, Ilana; Reuveni, Moshe; Azari, Raviv; Paran, Ilan; Nahon, Sahadia; Shlomo, Haviva; Chen, Lea; Lapidot, Moshe; Levin, Ilan

    2009-08-01

    Tomato yellow leaf curl virus (TYLCV) is devastating to tomato (Solanum lycopersicum) crops and resistant cultivars are highly effective in controlling the disease. The breeding line TY172, originating from Solanum peruvianum, is highly resistant to TYLCV. To map quantitative trait loci (QTLs) controlling TYLCV resistance in TY172, appropriate segregating populations were analyzed using 69 polymorphic DNA markers spanning the entire tomato genome. Results show that TYLCV resistance in TY172 is controlled by a previously unknown major QTL, originating from the resistant line, and four additional minor QTLs. The major QTL, we term Ty-5, maps to chromosome 4 and accounts for 39.7-46.6% of the variation in symptom severity among segregating plants (LOD score 33-35). The minor QTLs, originated either from the resistant or susceptible parents, were mapped to chromosomes 1, 7, 9 and 11, and contributed 12% to the variation in symptom severity in addition to Ty-5.

  2. Further isolation of AFLP and LMS markers for the mapping of the Ol-2 locus related to powdery mildew (Oidium neolycopersici) resistance in tomato (Solanum lycopersicum L.)

    NARCIS (Netherlands)

    Ricciardi, L.; Lotti, C.; Pavan, S.N.C.; Bai, Y.; Lindhout, P.; Giovanni, de C.

    2007-01-01

    Tomato powdery mildew (Oidium neolycopersici) is a new plant disease that in recent years has frequently occurred in open field and protected environments to cause serious damage to tomato crops. Currently, the development of resistant cultivars appear to be the best eco-compatible solution to contr

  3. Optimization of productivity and quality of irrigated tomato (Solanum lycopersicum L.) by smallholder farmers in the Central Rift Valley area of Oromia, Ethiopia

    NARCIS (Netherlands)

    Gemechis, Ambecha O.

    2017-01-01

    Tomato (Solanum lycopersicum L.) is a vegetable crop with high potential to contribute to poverty reduction via increased income and food security. It is widely grown by smallholders, has high productivity and its demand is increasing. Ethiopia produced about 30,700 Mg of tomatoes on 5,027 ha

  4. The role of endogenous strigolactones and their interaction with ABA during the infection process of the parasitic weed Phelipanche ramosa in tomato plants

    NARCIS (Netherlands)

    Cheng, Xi; Floková, Kristýna; Bouwmeester, Harro; Ruyter-Spira, Carolien

    2017-01-01

    The root parasitic plant species Phelipanche ramosa, branched broomrape, causes severe damage to economically important crops such as tomato. Its seed germination is triggered by host-derived signals upon which it invades the host root. In tomato, strigolactones (SLs) are the main germination

  5. Evaluation of tomato varieties for resistance to ozone

    Energy Technology Data Exchange (ETDEWEB)

    Clayberg, C.D.

    1972-01-01

    Tomatoes are among the crops most sensitive to ozone. Many researchers tested various varieties for resistance. The tests indicated a variation among tomato varieties for resistance to ozone and that some commercial varieties were as resistant as any lines tested. In the USA and Canada a total of 295 different varieties and hybrids were received and tested for response to ozone during the summer of 1971. Plants were exposed to O/sub 3/ when the first true leaves were 2 1/2 to 4 inches long. Tomato leaves showed typical injury symptoms following exposure to O/sub 3/. Mild symptoms were a chlorotic yellowing or whitening of tissue between the leaf veins, or small brownish necrotic flecks marginally or all over the leaf. Severe damage resulted in large water-soaked areas over the entire leaf surface right after exposure to ozone. Of the various varieties, the most resistant were Pierette and Heinz 1439 in successive tests. Susceptible ones were Roma VF and Fruhernte. Charkowskij and New Yorker were of an intermediate level of resistance. All the ozone-resistant varieties of KY 1, VFN 8, PI 304234, PI 309915, and Manzana (PI 203229) were about equally susceptible under the conditions of testing used. The range between the most resistant and the most susceptible tomato varieties was not as great as in crops like tobacco. 8 references, 2 tables.

  6. Characterization of culturable bacterial endophytes and their capacity to promote plant growth from plants grown using organic or conventional practices

    Science.gov (United States)

    Xia, Ye; DeBolt, Seth; Dreyer, Jamin; Scott, Delia; Williams, Mark A.

    2015-01-01

    Plants have a diverse internal microbial biota that has been shown to have an important influence on a range of plant health attributes. Although these endophytes have been found to be widely occurring, few studies have correlated agricultural production practices with endophyte community structure and function. One agricultural system that focuses on preserving and enhancing soil microbial abundance and biodiversity is organic farming, and numerous studies have shown that organically managed system have increased microbial community characteristics. Herein, the diversity and specificity of culturable bacterial endophytes were evaluated in four vegetable crops: corn, tomato, melon, and pepper grown under organic or conventional practices. Endophytic bacteria were isolated from surface-sterilized shoot, root, and seed tissues and sequence identified. A total of 336 bacterial isolates were identified, and grouped into 32 species and five phyla. Among these, 239 isolates were from organically grown plants and 97 from those grown conventionally. Although a diverse range of bacteria were documented, 186 were from the Phylum Firmicutes, representing 55% of all isolates. Using the Shannon diversity index, we observed a gradation of diversity in tissues, with shoots and roots having a similar value, and seeds having the least diversity. Importantly, endophytic microbial species abundance and diversity was significantly higher in the organically grown plants compared to those grown using conventional practices, potentially indicating that organic management practices may increase endophyte presence and diversity. The impact that these endophytes could have on plant growth and yield was evaluated by reintroducing them into tomato plants in a greenhouse environment. Of the bacterial isolates tested, 61% were found to promote tomato plant growth and 50–64% were shown to enhance biomass accumulation, illustrating their potential agroecosystem application. PMID:26217348

  7. Characterization of culturable bacterial endophytes and their capacity to promote plant growth from plants grown using organic or conventional practices.

    Science.gov (United States)

    Xia, Ye; DeBolt, Seth; Dreyer, Jamin; Scott, Delia; Williams, Mark A

    2015-01-01

    Plants have a diverse internal microbial biota that has been shown to have an important influence on a range of plant health attributes. Although these endophytes have been found to be widely occurring, few studies have correlated agricultural production practices with endophyte community structure and function. One agricultural system that focuses on preserving and enhancing soil microbial abundance and biodiversity is organic farming, and numerous studies have shown that organically managed system have increased microbial community characteristics. Herein, the diversity and specificity of culturable bacterial endophytes were evaluated in four vegetable crops: corn, tomato, melon, and pepper grown under organic or conventional practices. Endophytic bacteria were isolated from surface-sterilized shoot, root, and seed tissues and sequence identified. A total of 336 bacterial isolates were identified, and grouped into 32 species and five phyla. Among these, 239 isolates were from organically grown plants and 97 from those grown conventionally. Although a diverse range of bacteria were documented, 186 were from the Phylum Firmicutes, representing 55% of all isolates. Using the Shannon diversity index, we observed a gradation of diversity in tissues, with shoots and roots having a similar value, and seeds having the least diversity. Importantly, endophytic microbial species abundance and diversity was significantly higher in the organically grown plants compared to those grown using conventional practices, potentially indicating that organic management practices may increase endophyte presence and diversity. The impact that these endophytes could have on plant growth and yield was evaluated by reintroducing them into tomato plants in a greenhouse environment. Of the bacterial isolates tested, 61% were found to promote tomato plant growth and 50-64% were shown to enhance biomass accumulation, illustrating their potential agroecosystem application.

  8. Characterization of culturable bacterial endophytes and their capacity to promote plant growth from plants grown using organic or conventional practices

    Directory of Open Access Journals (Sweden)

    Ye eXia

    2015-07-01

    Full Text Available Plants have a diverse internal microbial biota that has been shown to have an important influence on a range of plant health attributes. Although these endophytes have been found to be widely occurring, few studies have correlated agricultural production practices with endophyte community structure and function. One agricultural system that focuses on preserving and enhancing soil microbial abundance and biodiversity is organic farming, and numerous studies have shown that organically managed system have increased microbial community characteristics. Herein, the diversity and specificity of culturable bacterial endophytes were evaluated in four vegetable crops: corn, tomato, melon and pepper grown under organic or conventional practices. Endophytic bacteria were isolated from surface-sterilized shoot, root and seed tissues and sequence identified. A total of 336 bacterial isolates were identified, and grouped into 32 species and 5 phyla. Among these, 239 isolates were from organically grown plants and 97 from those grown conventionally. Although a diverse range of bacteria were documented, 186 were from the Phylum Firmicutes, representing 55% of all isolates. Using the Shannon diversity index, we observed a gradation of diversity in tissues, with shoots and roots having a similar value, and seeds having the least diversity. Importantly, endophytic microbial species abundance and diversity was significantly higher in the organically grown plants compared to those grown using conventional practices, potentially indicating that organic management practices may increase endophyte presence and diversity. The impact that these endophytes could have on plant growth and yield was evaluated by reintroducing them into tomato plants in a greenhouse environment. Of the bacterial isolates tested, 61% were found to promote tomato plant growth and 50%-64% were shown to enhance biomass accumulation, illustrating their potential agroecosystem application.

  9. Actividades Enzimáticas en Consorcios Bacterianos de Suelos Bajo Cultivo de Papa con Manejo Convencional y Bajo Pastizal Enzyme Activities in Bacterial Consortium Isolated from Soils with Potato Crop under Conventional Management and under Grassland

    Directory of Open Access Journals (Sweden)

    Lizeth Manuela Avellaneda-Torres

    2012-06-01

    Full Text Available Resumen. Se evaluaron las actividades enzimáticas (ureasa, proteasa, fosfatasa ácida y alcalina, fosfodiesterasa, b-glucosidasa y arilsulfatasa en consorcios bacterianos (Bacillus subtilis, Brevundimonas diminuta, Flavimonas oryzihabitants de suelos bajo cultivo de papa variedad Parda Pastusa, con manejo convencional de aplicación de agroinsumos (PCA y en suelos bajo pastizal sin aplicación de agroinsumos (PSA, en fincas de tres localidades del departamento de Cundinamarca (Tausa, Villapinzón y Zipaquirá, Colombia. Se encontraron efectos por la aplicación de insumos de síntesis química y el tipo de uso del suelo, sobre las actividades enzimáticas; sin embargo, estos fueron diferentes para cada una de las enzimas y localidades. Para el municipio de Villapinzón la actividad de ureasa, fosfatasa ácida, fosfodiesterasa y b-glucosidasa, fue mayor en las muestras PCA con respecto a las PSA en un 89, 71, 67 y 75% respectivamente; para el municipio de Zipaquirá se presentó la misma tendencia en la actividad ureasa, b-glucosidasa y arilsulfatasa con un 50, 71 y 68% respectivamente; finalmente en el municipio de Tausa se mantuvo el mismo comportamiento para la actividad de proteasa, fosfatasa ácida, fosfatasa alcalina, fosfodiesterasa, b-glucosidasa, con un 55, 20, 75, 82 y 87% de mayor actividad en las muestras PCA en relación con las de PSA.Abstract. Enzyme activities were evaluated (urease, protease, acid phosphatase and alkaline phosphodiesterase, b-glucosidase and arylsulfatase in bacterial consortia (Bacillus subtilis, Brevundimonas diminuta, Flavimonas oryzihabitants from either soil with potato cropping under conventional management with the application of agrochemicals (PWA or grassland soils without the use of agrochemicals (GNA on farms of three municipalities (Tausa, Villapinzón and Zipaquirá in the department of Cundinamarca, Colombia. The type of land use and the location affected the tested enzymatic activities. In the

  10. Drought-Stressed Tomato Plants Trigger Bottom-Up Effects on the Invasive Tetranychus evansi.

    Science.gov (United States)

    Ximénez-Embún, Miguel G; Ortego, Félix; Castañera, Pedro

    2016-01-01

    Climate change will bring more drought periods that will have an impact on the irrigation practices of some crops like tomato, from standard water regime to deficit irrigation. This will promote changes in plant metabolism and alter their interactions with biotic stressors. We have tested if mild or moderate drought-stressed tomato plants (simulating deficit irrigation) have an effect on the biological traits of the invasive tomato red spider mite, Tetranychus evansi. Our data reveal that T evansi caused more leaf damage to drought-stressed tomato plants (≥1.5 fold for both drought scenarios). Mite performance was also enhanced, as revealed by significant increases of eggs laid (≥2 fold) at 4 days post infestation (dpi), and of mobile forms (≥2 fold and 1.5 fold for moderate and mild drought, respectively) at 10 dpi. The levels of several essential amino acids (histidine, isoleucine, leucine, tyrosine, valine) and free sugars in tomato leaves were significantly induced by drought in combination with mites. The non-essential amino acid proline was also strongly induced, stimulating mite feeding and egg laying when added to tomato leaf disks at levels equivalent to that estimated on drought-infested tomato plants at 10 dpi. Tomato plant defense proteins were also affected by drought and/or mite infestation, but T. evansi was capable of circumventing their potential adverse effects. Altogether, our data indicate that significant increases of available free sugars and essential amino acids, jointly with their phagostimulant effect, created a favorable environment for a better T. evansi performance on drought-stressed tomato leaves. Thus, drought-stressed tomato plants, even at mild levels, may be more prone to T evansi outbreaks in a climate change scenario, which might negatively affect tomato production on area-wide scales.

  11. Role and regulation of autophagy in heat stress responses of tomato plants.

    Science.gov (United States)

    Zhou, Jie; Wang, Jian; Yu, Jing-Quan; Chen, Zhixiang

    2014-01-01

    As sessile organisms, plants are constantly exposed to a wide spectrum of stress conditions such as high temperature, which causes protein misfolding. Misfolded proteins are highly toxic and must be efficiently removed to reduce cellular proteotoxic stress if restoration of native conformations is unsuccessful. Although selective autophagy is known to function in protein quality control by targeting degradation of misfolded and potentially toxic proteins, its role and regulation in heat stress responses have not been analyzed in crop plants. In the present study, we found that heat stress induced expression of autophagy-related (ATG) genes and accumulation of autophagosomes in tomato plants. Virus-induced gene silencing (VIGS) of tomato ATG5 and ATG7 genes resulted in increased sensitivity of tomato plants to heat stress based on both increased development of heat stress symptoms and compromised photosynthetic parameters of heat-stressed leaf tissues. Silencing of tomato homologs for the selective autophagy receptor NBR1, which targets ubiquitinated protein aggregates, also compromised tomato heat tolerance. To better understand the regulation of heat-induced autophagy, we found that silencing of tomato ATG5, ATG7, or NBR1 compromised heat-induced expression of not only the targeted genes but also other autophagy-related genes. Furthermore, we identified two tomato genes encoding proteins highly homologous to Arabidopsis WRKY33 transcription factor, which has been previously shown to interact physically with an autophagy protein. Silencing of tomato WRKY33 genes compromised tomato heat tolerance and reduced heat-induced ATG gene expression and autophagosome accumulation. Based on these results, we propose that heat-induced autophagy in tomato is subject to cooperative regulation by both WRKY33 and ATG proteins and plays a critical role in tomato heat tolerance, mostly likely through selective removal of heat-induced protein aggregates.

  12. Role and Regulation of Autophagy in Heat Stress Responses of Tomato Plants

    Directory of Open Access Journals (Sweden)

    Jie eZhou

    2014-04-01

    Full Text Available As sessile organisms, plants are constantly exposed to a wide spectrum of stress conditions such as high temperature, which causes protein misfolding. Misfolded proteins are highly toxic and must be efficiently removed to reduce cellular proteotoxic stress if restoration of native conformations is unsuccessful. Although selective autophagy is known to function in protein quality control by targeting degradation of misfolded and potentially toxic proteins, its role and regulation in heat stress responses have not been analyzed in crop plants. In the present study, we found that heat stress induced expression of autophagy-related (ATG genes and accumulation of autophagosomes in tomato plants. Virus-induced gene silencing of tomato ATG5 and ATG7 genes resulted in increased sensitivity of tomato plants to heat stress based on both increased development of heat stress symptoms and compromised photosynthetic parameters of heat-stressed leaf tissues. Silencing of tomato homologs for the selective autophagy receptor NBR1, which targets ubiquitinated protein aggregates, also compromised tomato heat tolerance. To better understand the regulation of heat-induced autophagy, we found that silencing of tomato ATG5, ATG7 or NBR1 compromised heat-induced expression of not only the targeted genes but also other autophagy-related genes. Furthermore, we identified two tomato genes encoding proteins highly homologous to Arabidopsis WRKY33 transcription factor, which has been previously shown to interact physically with an autophagy protein. Silencing of tomato WRKY33 genes compromised tomato heat tolerance and reduced heat-induced ATG gene expression and autophagosome accumulation. Based on these results, we propose that heat-induced autophagy in tomato is subject to cooperative regulation by both WRKY33 and ATG proteins and plays a critical role in tomato heat tolerance, mostly likely through selective removal of heat-induced protein aggregates.

  13. Drought-Stressed Tomato Plants Trigger Bottom-Up Effects on the Invasive Tetranychus evansi.

    Directory of Open Access Journals (Sweden)

    Miguel G Ximénez-Embún

    Full Text Available Climate change will bring more drought periods that will have an impact on the irrigation practices of some crops like tomato, from standard water regime to deficit irrigation. This will promote changes in plant metabolism and alter their interactions with biotic stressors. We have tested if mild or moderate drought-stressed tomato plants (simulating deficit irrigation have an effect on the biological traits of the invasive tomato red spider mite, Tetranychus evansi. Our data reveal that T evansi caused more leaf damage to drought-stressed tomato plants (≥1.5 fold for both drought scenarios. Mite performance was also enhanced, as revealed by significant increases of eggs laid (≥2 fold at 4 days post infestation (dpi, and of mobile forms (≥2 fold and 1.5 fold for moderate and mild drought, respectively at 10 dpi. The levels of several essential amino acids (histidine, isoleucine, leucine, tyrosine, valine and free sugars in tomato leaves were significantly induced by drought in combination with mites. The non-essential amino acid proline was also strongly induced, stimulating mite feeding and egg laying when added to tomato leaf disks at levels equivalent to that estimated on drought-infested tomato plants at 10 dpi. Tomato plant defense proteins were also affected by drought and/or mite infestation, but T. evansi was capable of circumventing their potential adverse effects. Altogether, our data indicate that significant increases of available free sugars and essential amino acids, jointly with their phagostimulant effect, created a favorable environment for a better T. evansi performance on drought-stressed tomato leaves. Thus, drought-stressed tomato plants, even at mild levels, may be more prone to T evansi outbreaks in a climate change scenario, which might negatively affect tomato production on area-wide scales.

  14. The Tomato Expression Atlas.

    Science.gov (United States)

    Fernandez-Pozo, Noe; Zheng, Yi; Snyder, Stephen I; Nicolas, Philippe; Shinozaki, Yoshihito; Fei, Zhangjun; Catala, Carmen; Giovannoni, James J; Rose, Jocelyn K C; Mueller, Lukas A

    2017-08-01

    With the development of new high-throughput DNA sequencing technologies and decreasing costs, large gene expression datasets are being generated at an accelerating rate, but can be complex to visualize. New, more interactive and intuitive tools are needed to visualize the spatiotemporal context of expression data and help elucidate gene function. Using tomato fruit as a model, we have developed the Tomato Expression Atlas to facilitate effective data analysis, allowing the simultaneous visualization of groups of genes at a cell/tissue level of resolution within an organ, enhancing hypothesis development and testing in addition to candidate gene identification. This atlas can be adapted to different types of expression data from diverse multicellular species. The Tomato Expression Atlas is available at http://tea.solgenomics.net/ . Source code is available at https://github.com/solgenomics/Tea . jr286@cornell.edu or lam87@cornell.edu. Supplementary data are available at Bioinformatics online.

  15. Association Mapping of Main Tomato Fruit Sugars and Organic Acids

    Science.gov (United States)

    Zhao, Jiantao; Xu, Yao; Ding, Qin; Huang, Xinli; Zhang, Yating; Zou, Zhirong; Li, Mingjun; Cui, Lu; Zhang, Jing

    2016-01-01

    Association mapping has been widely used to map the significant associated loci responsible for natural variation in complex traits and are valuable for crop improvement. Sugars and organic acids are the most important metabolites in tomato fruits. We used a collection of 174 tomato accessions composed of Solanum lycopersicum (123 accessions) and S. lycopersicum var cerasiforme (51 accessions) to detect significantly associated loci controlling the variation of main sugars and organic acids. The accessions were genotyped with 182 SSRs spreading over the tomato genome. Association mapping was conducted on the main sugars and organic acids detected by gas chromatography-mass spectrometer (GC-MS) over 2 years using the mixed linear model (MLM). We detected a total of 58 significantly associated loci (P organic acids, including fructose, glucose, sucrose, citric acid, malic acid. These results not only co-localized with several reported QTLs, including fru9.1/PV, suc9.1/PV, ca2.1/HS, ca3.1/PV, ca4.1/PV, and ca8.1/PV, but also provided a list of candidate significantly associated loci to be functionally validated. These significantly associated loci could be used for deciphering the genetic architecture of tomato fruit sugars and organic acids and for tomato quality breeding. PMID:27617019

  16. Development and regulation of pedicel abscission in tomato

    Directory of Open Access Journals (Sweden)

    Yasuhiro eIto

    2015-06-01

    Full Text Available To shed unfertilized flowers or ripe fruits, many plant species develop a pedicel abscission zone (AZ, a specialized tissue that develops between the organ and the main body of the plant. Regulation of pedicel abscission is an important agricultural concern because pre-harvest abscission can reduce yields of fruit or grain crops, such as apples, rice, wheat, etc. Tomato has been studied as a model system for abscission, as tomato plants develop a distinct AZ at the midpoint of the pedicel and several tomato mutants, such as jointless, have pedicels that lack an AZ. This mini-review focuses on recent advances in research on the mechanisms regulating tomato pedicel abscission. Molecular genetic studies revealed that three MADS-box transcription factors interactively play a central role in pedicel AZ development. Transcriptome analyses identified activities involved in abscission and also found novel transcription factors that may regulate AZ activities. Another study identified transcription factors mediating abscission pathways from induction signals to activation of cell wall hydrolysis. These recent findings in tomato will enable significant advances in understanding the regulation of abscission in other key agronomic species.

  17. Quantitative losses in mechanized harvesting corn crop in small footprint and conventionalPerdas quantitativas na colheita mecanizada do milho cultivado em espaçamentos reduzido e convencional

    Directory of Open Access Journals (Sweden)

    Daniel Mariano Leite

    2012-05-01

    Full Text Available The objective of this study was to evaluate the quantitative losses occurred in the mechanical harvesting of the corn grown in conventional and reduced gaps. The experimental design was randomized blocks with three replications and treatments distributed in a factorial (2 x 3 x 3, and two spacings (0.45 m and 0.90 m, three speeds of the combine (1.8 km.h-1 3.5 km.h-1 and 4.1 km.h-1 and three openings of the concave (25 mm, 30 mm and 35 mm. For treatments where the spacing was 0.45 m, the harvester worked with a power of two lines dividing line between the platform crop while in treatments where the spacing was 0.90 m, the harvester worked with a power dividing line between a line of platform. The plant population was the same for the two spacings used in the experiment. The natural losses were higher in the conventional spacing, but the header losses of harvest had the lowest use this space at a speed of 3.5 km.h-1. As maiores perdas ocorreram nos mecanismos internos da colhedora ocorreu quando se utiliza a abertura côncava de 30 mm a uma velocidade de 3,5 km.h-1. Total losses accounted for 8.2% and 7.3% of the average productivity in the gaps of 0.45 m 0.9 m respectivelyObjetivou-se com este trabalho, avaliar as perdas quantitativas ocorridas na colheita mecanizada do milho cultivado em espaçamentos reduzido e convencional. O delineamento experimental foi o de blocos casualizados com três repetições em esquema fatorial (2 x 3 x 3, sendo dois espaçamentos ( 0,45m e 0,90m: três velocidades da colhedora (1,8 km.h-1 3,5 km.h-1 e 4,1 km.h-1 e três aberturas do côncavo (25 mm, 30 mm e 35 mm. Para os tratamentos onde o espaçamento entre linhas foi de 0,45 m, a colhedora trabalhou com uma alimentação de duas linhas entre os divisores de linha da plataforma de colheita enquanto nos tratamentos onde o espaçamento foi de 0,90 m, a colhedora trabalhou com uma alimentação de uma linha entre os divisores de linha da plataforma. A popula

  18. 21 CFR 155.191 - Tomato concentrates.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Tomato concentrates. 155.191 Section 155.191 Food... Tomato concentrates. (a) Identity—(1) Definition. Tomato concentrates are the class of foods each of... tomato ingredients: (i) The liquid obtained from mature tomatoes of the red or reddish...

  19. Peeling of tomatoes using novel infrared radiation heating technology

    Science.gov (United States)

    The effectiveness of using infrared (IR) dry-peeling as an alternative process for peeling tomatoes without lye and water was studied. Compared to conventional lye peeling, IR dry-peeling using 30 s to 75 s heating time resulted in lower peeling loss (8.3% - 13.2% vs. 12.9% - 15.8%), thinner thickne...

  20. Genetic characterization of Italian tomato varieties and their traceability in tomato food products-Sardaro-2012-Food Science & Nutrition-Wiley Online Library.

    Science.gov (United States)

    Sardaro, Maria Luisa Savo; Marmiroli, Marta; Maestri, Elena; Marmiroli, Nelson

    2013-01-01

    Genetic diversity underlies the improvement of crops by plant breeding. Landraces of tomato (Solanum lycopersicum L.) can contain valuable alleles not common in modern germplasms. The aim was to measure genetic diversity present in 47 most common tomato varieties grown in Italy, 35 were varieties used for processing and 12 were landraces considered "salad varieties". Furthermore, we demonstrated the possibility that the variety traceability can be extended through the entire production chain. Diversity was measured using 11 microsatellite markers and 94 genotypes. Among the markers used, a total of 48 alleles were detected. A dendrogram based on total microsatellite polymorphism grouped 47 varieties into three major clusters at 0.75 similarity coefficient, differentiating the modern varieties from tomatoes landraces. The DNA markers developed confirmed the possibility to support the genotype identification all along the tomato production chain. The number of alleles and genotypes identified in the present work is the largest considering papers on food traceability.

  1. Recessive Resistance Derived from Tomato cv. Tyking-Limits Drastically the Spread of Tomato Yellow Leaf Curl Virus

    Directory of Open Access Journals (Sweden)

    Rita C. Pereira-Carvalho

    2015-05-01

    Full Text Available The tomato yellow leaf curl disease (TYLCD causes severe damage to tomato (Solanum lycopersicum L. crops throughout tropical and subtropical regions of the world. TYLCD is associated with a complex of single-stranded circular DNA plant viruses of the genus Begomovirus (family Geminiviridae transmitted by the whitefy Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae. The tomato inbred line TX 468-RG is a source of monogenic recessive resistance to begomoviruses derived from the hybrid cv. Tyking F1. A detailed analysis of this germplasm source against tomato yellow leaf curl virus-Israel (TYLCV-IL, a widespread TYLCD-associated virus, showed a significant restriction to systemic virus accumulation even under continuous virus supply. The resistance was effective in limiting the onset of TYLCV-IL in tomato, as significantly lower primary spread of the virus occurred in resistant plants. Also, even if a limited number of resistant plants could result infected, they were less efficient virus sources for secondary spread owing to the impaired TYLCV-IL accumulation. Therefore, the incorporation of this resistance into breeding programs might help TYLCD management by drastically limiting TYLCV-IL spread.

  2. Negative effects of fluoranthene on the ecophysiology of tomato plants (Lycopersicon esculentum Mill) Fluoranthene mists negatively affected tomato plants.

    Science.gov (United States)

    Oguntimehin, Ilemobayo; Eissa, Fawzy; Sakugawa, Hiroshi

    2010-02-01

    Cherry tomato plants (Lycopersicon esculentum Mill) were sprayed with fluoranthene and mixture of fluoranthene and mannitol solutions for 30d. The exposure was carried out in growth chambers in field conditions, and the air was filtered through charcoal filters to remove atmospheric contaminants. Plants were sprayed with 10microM fluoranthene as mist until they reached the fruiting stage, and the eco-physiological parameters were measured to determine the effects of the treatments. We measured CO(2) uptake and water vapour exchange, chlorophyll fluorescence, leaf pigment contents, visual symptoms and biomass allocation. Fluoranthene which was deposited as mist onto leaves negatively affected both growth and the quality of tomato plants, while other treatments did not. The photosynthetic rate measured at saturated irradiance was approximately 37% lower in fluoranthene-treated plants compared with the control group. Other variables, such as stomata conductance, the photochemical efficiency of PSII in the dark, Chl a, Chl b, and the total chlorophyll contents of the tomato leaves were significantly reduced in the fluoranthene-treated plants. Tomato plants treated with fluoranthene showed severe visible injury symptoms on the foliage during the exposure period. Mannitol (a reactive oxygen scavenger) mitigated effects of fluoranthene; thus, reactive oxygen species generated through fluoranthene may be responsible for the damaged tomato plants. It is possible for fluoranthene to decrease the aesthetic and hence the economic value of this valuable crop plant.

  3. Mulch materials in processing tomato: a multivariate approach

    OpenAIRE

    Marta María Moreno; Carmen Moreno; Ana María Tarquis

    2013-01-01

    Mulch materials of different origins have been introduced into the agricultural sector in recent years alternatively to the standard polyethylene due to its environmental impact. This study aimed to evaluate the multivariate response of mulch materials over three consecutive years in a processing tomato (Solanum lycopersicon L.) crop in Central Spain. Two biodegradable plastic mulches (BD1, BD2), one oxo-biodegradable material (OB), two types of paper (PP1, PP2), and one barley straw cover (B...

  4. Tomato fruits: a good target for iodine biofortification

    OpenAIRE

    Claudia eKiferle; Silvia eGonzali; Harmen Tjalling Holwerda; Rodrigo eReal Ibaceta; Pierdomenico ePerata

    2013-01-01

    Iodine is a trace element that is fundamental for human health: its deficiency affects about two billion people worldwide. Fruits and vegetables are usually poor sources of iodine; however plants can accumulate iodine if it is either present or exogenously administered to the soil. The biofortification of crops with iodine has therefore been proposed as a strategy for improving human nutrition. A greenhouse pot experiment was carried out to evaluate the possibility of biofortifying tomato fru...

  5. CROPS Clever Robots for Crops

    NARCIS (Netherlands)

    Bontsema, J.; Hemming, J.; Pekkeriet, E.J.

    2015-01-01

    In the EU-funded CROPS project robots are developed for site-specific spraying and selective harvesting of fruit
    and fruit vegetables. The robots are being designed to harvest crops, such as greenhouse vegetables, apples,
    grapes and for canopy spraying in orchards and for precision target sp

  6. 不同氮肥及有机肥投入对设施番茄菜地AM真菌侵染势和空间分布的影响%Effects of Different Nitrogen and Organic Manure Application on Infection Potential and Spatial Distribution of Arbuscular Mycorrhizal Fungi in Greenhouse Tomato Cropping System

    Institute of Scientific and Technical Information of China (English)

    李灵芝; 行园园; 盖京苹; 王艳芳; 李海平

    2015-01-01

    The present study targeted in the long-term experiment in a greenhouse tomato(Solanum lyco-persicum L.)double-cropping system in Shandong Province. We collected soil from different soil layers of the plots,identified AMF spore community,and established the bioassay in the greenhouse. The results showed that there were still certain amount of AM fungi in the greenhouse vegetable fields,although different nutrient inputs changed soil AM fungal community composition by a certain degree. AM fungal infectivity was greatly reduced by high amount of fertilizer use no significant differences were found in spatial distribution of AM fungi among different soil layers.%在山东寿光一年两季设施番茄长期定位施肥试验的基础上,检测土壤中的AM真菌群落组成;并通过生物接种的方法,检测不同土层AM真菌侵染势(MIP),研究不同氮肥和有机肥投入对土壤中AM真菌繁殖体数量和生物效应的影响。结果表明,设施菜田中不同养分投入可在一定程度上改变土壤AM真菌群落结构,但仍能保持一定数量的AM真菌。施肥量的增加显著抑制了AM真菌侵染势,AM真菌在不同土层的空间分布差异不显著。

  7. Veggies in Space: Salad Crop Production on the ISS

    Science.gov (United States)

    Massa, Gioia

    2016-01-01

    NASA is currently testing Veggie, a low mass, low energy, salad crop production system on the International Space Station (ISS). Veggie grows crops with LED lights using ISS cabin air and passive watering that has presented challenges in microgravity. Initial tests included red romaine lettuce and zinnia, with testing of Chinese cabbage, and tomatoes planned. A goal is to add supplemental salad foods to the astronaut diet as we prepare for a future journey to Mars.

  8. Preliminary design, construction and evaluation of robot of tomato seed planting for the trays of greenhouse

    Directory of Open Access Journals (Sweden)

    J Ghezavati

    2015-09-01

    Full Text Available Introduction: From an economic viewpoint, tomato is considered as the second most valuable crop after potato. It is also preceded by the potato in terms of per capita consumption in the world. In 2008, the cultivation area used for the tomato as equal to 163,539 hectares in Iran and the production of it was equal to 5,887,715 tons with an average production of 117,887 tons in 4352 hectares in the provinces, respectively. Having high production volume and quality, costly hybrid seeds are currently used for the major planting areas of vegetable in Iran. Most of the used transplanted seedlings are 83%. Since the seeds are expensive, the percentage of seedlings and healthy and disease-free seeds should be used for maximized germination and be transferred to the fields of open space. Preparing seedlings in transplanting trays is a technology to respond to this need. Trays are covered with a layer of Peat and Miculite fertilizers. Then, one seed is manually placed in each cell after gauging and preparing a suitable field. However, manually placing seeds is time-consuming and requires hard labor. Sixteen working labors per hour are required for 15 × 7 cell in order to have 10200 seedlings grown in 100 trays. Due to lack of adequate labor, production capacity of greenhouses is reduced, especially in the farming season when finding labor for planting vegetable sprouts is laborious. Therefore, mechanizing tray seeding operations is essential to increase the capacity of the growing industry of greenhouses in Iran. Materials and Methods: Initially, the tomato seeds were examined in the laboratory. The most important parameters of the study included size, shape, weight, the speed of getting out of the tank and the minimum carrying speed. Then, a vacuum-based single seed picking unit was prepared to investigate the factors influencing the design, so that a single tomato seed can be harvested from the masses. The most important factors considered in the

  9. Acid phosphatase-1 1, a molecular marker tightly linked to root-knot nematode resistance in tomato.

    NARCIS (Netherlands)

    Aarts, J.M.M.J.G.

    1993-01-01

    Root knot nematode resistance in tomato is a genetic trait which is determined by a single dominant gene ( Mi ) on chromosome 6 of tomato. Information about the mRNA or protein product is completely lacking, which precludes the cloning of Mi by conventional strategies based on gene expression. Howev

  10. Tips on Tomatoes

    OpenAIRE

    Extension, USU

    2007-01-01

    Organisms that cause food spoilage - molds, yeasts and bacteria - are always present in the air, water and soil. Enzymes that may cause undesirable changes in flavor, color and texture are present in raw fruits. When tomatoes are canned, they are heated hot enough and long enough to destroy spoilage organisms.

  11. Solanum lycopersicum (Tomato)

    NARCIS (Netherlands)

    Pavan, S.N.C.; Heusden, van A.W.; Bai, Y.

    2009-01-01

    After its introduction in Europe the tomato (Solanum lycopersicum) has gone a long way. Dedicated breeding has resulted in numerous cultivars grown all over the world, differing in all kind of aspects such as yield, shape, resistance, taste and quality. Modern cultivars are sold as hybrids with a

  12. The tomato genome

    Science.gov (United States)

    The tomato genome sequence was undertaken at a time when state-of-the-art sequencing methodologies were undergoing a transition to co-called next generation methodologies. The result was an international consortium undertaking a strategy merging both old and new approaches. Because biologists were...

  13. Solanum lycopersicum (Tomato)

    NARCIS (Netherlands)

    Pavan, S.N.C.; Heusden, van A.W.; Bai, Y.

    2009-01-01

    After its introduction in Europe the tomato (Solanum lycopersicum) has gone a long way. Dedicated breeding has resulted in numerous cultivars grown all over the world, differing in all kind of aspects such as yield, shape, resistance, taste and quality. Modern cultivars are sold as hybrids with a ve

  14. CROP FARMIERS

    African Journals Online (AJOL)

    however, their success in addressing the credit needs of food crop farmers remains a ..... will to carry such policies through in the match .... farmer, his mental capacity to cope with the daily .... had stated that farm level credit when properly.

  15. Environmental and economic assessment of protected crops in four European scenarios

    NARCIS (Netherlands)

    Torrellas, M.; Antón, A.; Ruijs, M.N.A.; Garcia Victoria, N.; Stanghellini, C.; Montero, J.I.

    2012-01-01

    In this study we analysed the environmental and economic profile of current agricultural practices for greenhouse crops, in cold and warm climates in Europe, using four scenarios as reference systems: tomato crop in a plastic greenhouse in Spain, and in glasshouses in Hungary and the Netherlands,

  16. The significance of crop co-states for receding horizon optimal control of greenhouse climate

    NARCIS (Netherlands)

    Straten, van G.; Willigenburg, van L.G.; Tap, R.F.

    2002-01-01

    While a tomato crop grows on the time-scale of weeks, the greenhouse climate changes on a time-scale of minutes. The economic optimal control problem of producing good quality crops against minimum input of resources is tackled by a two time-scale decomposition. First, the sub-problem associated to

  17. 1978 Insect Pest Management Guide: Commercial Vegetable Crops and Greenhouse Vegetables. Circular 897.

    Science.gov (United States)

    Illinois Univ., Urbana. Cooperative Extension Service.

    This circular lists suggested uses of insecticides for the control of pests by commercial vegetable farmers. Suggestions are given for selection, dosage and application of insecticides to control pests of cabbage and related crops, beans, cucumbers and other vine crops, tomatoes, potatoes, peppers, corn, and onions. (CS)

  18. Environmental and economic assessment of protected crops in four European scenarios

    NARCIS (Netherlands)

    Torrellas, M.; Antón, A.; Ruijs, M.N.A.; Garcia Victoria, N.; Stanghellini, C.; Montero, J.I.

    2012-01-01

    In this study we analysed the environmental and economic profile of current agricultural practices for greenhouse crops, in cold and warm climates in Europe, using four scenarios as reference systems: tomato crop in a plastic greenhouse in Spain, and in glasshouses in Hungary and the Netherlands, an

  19. Evaluation of gypsum rates on greenhouse crop production

    Science.gov (United States)

    This study was to determine the potential of an added value distribution channel for gypsum waste by evaluating various greenhouse crops with captious pH and calcium needs. Three studies consisting of: Zonal geranium (Pelargonium x hortorum) and petunia (Petunia x hybrida); tomato (Solanum lycoper...

  20. Transgenic tomato hybrids resistant to tomato spotted wilt virus infection.

    OpenAIRE

    Haan; Ultzen, T.; Prins, M.; Gielen, J.; Goldbach, R.; Grinsven, van, H.

    1996-01-01

    Tomato spotted wilt virus (TSWV) infections cause significant economic losses in the commercial culture of tomato (Lycopersicon esculentum). Culture practices have only been marginally effective in controlling TSWV. The ultimate way to minimize losses caused by TSWV is resistant varieties. These can be obtained by introgression of natural sources of resistance from wild relatives or by expressing viral sequences in transgenic tomato plants. We report high levels of resistance to TSWV obtained...

  1. Transgenic tomato hybrids resistant to tomato spotted wilt virus infection.

    OpenAIRE

    Haan, de, A.; Ultzen, T.; Prins, M.; Gielen, J; Goldbach, R; Grinsven, van, J.J.M.

    1996-01-01

    Tomato spotted wilt virus (TSWV) infections cause significant economic losses in the commercial culture of tomato (Lycopersicon esculentum). Culture practices have only been marginally effective in controlling TSWV. The ultimate way to minimize losses caused by TSWV is resistant varieties. These can be obtained by introgression of natural sources of resistance from wild relatives or by expressing viral sequences in transgenic tomato plants. We report high levels of resistance to TSWV obtained...

  2. Water requirement and irrigation schedule for tomato in northern guinea savanna zone, Nigeria

    Directory of Open Access Journals (Sweden)

    Ibraheem Alhassan

    2015-06-01

    Full Text Available Assessment of water requirement and irrigation schedule for tomato with the support of FAO-CROPWAT simulation model was carried out for Yola, Nigeria with the aim of planning irrigation schedules for tomato and develop recommendations for improve irrigation practices. The climatic data for 2012/2013 and soil properties of the study area were input into the program. Tomato crop properties were updated by the FAO data and three irrigation intervals were tested (7 and 10 days irrigation intervals and irrigation schedule of 10 days interval during initial and development stage and 6 days interval at mid and late season stages of tomato crop. The simulated results analysis for tomato according to the irrigation schedule showed that highest yield reduction of 16.2% was recorded with 10 days irrigation interval treatment and the least of 0.4% with irrigation interval of 10 days at first two growth stages and 6 days at last two stages. FAO-CROPWAT 8.0 can be used in planning proper irrigation schedule for tomato in Yola, Nigeria.

  3. Effects of Red Light Night Break Treatment on Growth and Flowering of Tomato Plants

    Directory of Open Access Journals (Sweden)

    Kai eCao

    2016-04-01

    Full Text Available Compact and healthy young plants increase crop production and improve vegetable quality. Adverse climatic conditions and shading can cause young plants to become elongated and spindly. We investigated the effects of night break (NB treatments on tomato plants using red light (RL with an intensity of 20 µmol•m2•s-1. Tomato plants were subjected to NB treatments with different frequencies ranging from every 1, 2, 3, and 4 h, and plant growth, flowering, and yield were monitored. The results showed that with the increase of RL NB frequency, plant height decreased, stem diameter increased, and flower initiation delayed, the content of indole-3-acetic acid (IAA and gibberellin 3 (GA3 in the leaf and stem declined. When the RL NB frequency was every 1 h, the heights of tomato plant decreased by 32.73% compared with the control, the diameter of tomato plants increased by 27.09% compared with the control, the number of leaves produced before flowering increased to 11, compared with 8 in the control, the contents of IAA and GA3 in the leaf decreased by 33.3% and 41.29% respectively compared with the control, the contents of IAA and GA3 in the stem decreased by 56.04% and 57.14% respectively compared with the control. After RL NB treatments, tomato plants were transplanted into a solar greenhouse to evaluate tomato yield. When tomato plants pre-treated with RL NB, per tomato fresh weight of the first spica increased with the increase of RL NB frequencies. These results indicate that more compact and healthier tomato plants could be gotten by RL NB treatments and improve tomato early yield.

  4. Effects of Red Light Night Break Treatment on Growth and Flowering of Tomato Plants.

    Science.gov (United States)

    Cao, Kai; Cui, Lirong; Ye, Lin; Zhou, Xiaoting; Bao, Encai; Zhao, Hailiang; Zou, Zhirong

    2016-01-01

    Compact and healthy young plants increase crop production and improve vegetable quality. Adverse climatic conditions and shading can cause young plants to become elongated and spindly. We investigated the effects of night break (NB) treatments on tomato plants using red light (RL) with an intensity of 20 μmol·m(2)·s(-1). Tomato plants were subjected to NB treatments with different frequencies ranging from every 1, 2, 3, and 4 h, and plant growth, flowering, and yield were monitored. The results showed that with the increase of RL NB frequency, plant height decreased, stem diameter increased, and flower initiation delayed, the content of indole-3-acetic acid (IAA) and gibberellin 3 (GA3) in the leaf and stem declined. When the RL NB frequency was every 1 h, the heights of tomato plant decreased by 32.73% compared with the control, the diameter of tomato plants increased by 27.09% compared with the control, the number of leaves produced before flowering increased to 11, compared with 8 in the control, the contents of IAA and GA3 in the leaf decreased by 33.3 and 41.29% respectively compared with the control, the contents of IAA and GA3 in the stem decreased by 56.04 and 57.14% respectively compared with the control. After RL NB treatments, tomato plants were transplanted into a solar greenhouse to evaluate tomato yield. When tomato plants pre-treated with RL NB, per tomato fresh weight of the first spica increased with the increase of RL NB frequencies. These results indicate that more compact and healthier tomato plants could be gotten by RL NB treatments and improve tomato early yield.

  5. Consumer sensory analysis of organically and conventionally grown vegetables.

    Science.gov (United States)

    Zhao, Xin; Chambers, Edgar; Matta, Ziad; Loughin, Thomas M; Carey, Edward E

    2007-03-01

    Consumers of organically grown fruits and vegetables often believe that these products taste better than conventional produce. However, comparison of produce from supermarket shelves does not permit adequate assessment of this consumer perception, given potentially confounding cultivar and environmental effects. We used replicated side-by-side plots to produce organic and conventional vegetables for consumer sensory studies. In one test, red loose leaf lettuce, spinach, arugula, and mustard greens, grown organically and conventionally, were evaluated for overall liking as well as for intensity of flavor and bitterness. Another consumer test was conducted comparing organically and conventionally grown tomatoes, cucumbers, and onions. Overall, organically and conventionally grown vegetables did not show significant differences in consumer liking or consumer-perceived sensory quality. The only exception was in tomatoes where the conventionally produced tomato was rated as having significantly stronger flavor than the organically produced tomato. However, overall liking was the same for both organic and conventional samples. As conventional tomatoes also were scored marginally significantly higher in ripeness and a positive correlation was found between ratings of flavor intensity and ripeness, the flavor difference observed could not be simply ascribed to the contrasting growing conditions. Consumer panelists in both tests considered organic produce to be healthier (72%) and more environmentally friendly (51%) than conventional produce, while 28% considered organic produce to have better taste. Covariance analysis indicated that consumer demographics affected sensory comparisons of organic and conventional lettuce and cucumbers. Future study is needed to substantiate the influence of segmentation of consumers on their preference for organic food.

  6. Water Use Efficiency under Different Tillage and Irrigation Systems for Tomato Farming in Southeastern Brazil

    Science.gov (United States)

    Bhering, S. B.; Fernandes, N. F.; Macedo, J. R.

    2009-04-01

    highly degrade the environment, applied without practices of soil and water conservation. Such production systems are associated with a variety of environmental problems, such as soil erosion, the extensive pumping of groundwater, the partial obstruction of surface drainage to form artificial lakes, the contamination of groundwater, among others. The environmental impacts generated by all these problems assume a greater importance due to the complete absence of monitoring the continuous lowering of the water table and the changes in water quality. We consider that the main management strategies for developing sustainable production systems for the tomato farming in this area should be based on monitoring water use efficiency, increasing water availability in the root zone and also preventing runoff, leaching and evaporation of water from the soil. Therefore, techniques were applied as green manures with legumes without incorporation of the biomass, non-mechanized and curve-level soil preparation, planting in level, soil cover with crop residues, fertirrigation with solid fertilization of low value, the conduct of tomato especially supported by plastic string attached to a trellis, drip irrigation, and monitoring soil water potential (SWP) with Watermak sensors. At the end of the tomato cycle, water use efficiency and the productivity were compared at 8 micro-plots installed in the 3 studied production systems: conventional tillage (CT-H), minimum tillage (MT-H), both with "wetting irrigation with garden hose", and no-tillage with drip irrigation (NT-D). For each production system, soil physical properties were characterized and soil water potential (SWP) and soil temperature were continuously monitored at different depths (20, 40, 60 and 80 cm), as well as the total water volume used in each irrigation. In parallel, we also compared the development of the root system and the final productivity for each one of the three production systems. The results obtained in this

  7. Herbaceous energy crops: planning for a renewed commitment. [Hay, silage, rapeseed, sugar and starch crops, hydrocarbon crops

    Energy Technology Data Exchange (ETDEWEB)

    Berger, B.J.; Cushman, J.H.

    1984-01-01

    In 1984, the US Department of Energy's Biomass Energy Technology Division (BETD) began a new program of research on the production of herbaceous crops for energy. In addition to the new Herbaceous Energy Crops (HEC) Program, ongoing BETD programs involve woody and aquatic energy crops. The goal of the HEC Program is to provide the technology base that will allow industry to develop commercially viable species and systems in order to produce herbaceous biomass for fuels and energy feedstocks. The program will concentrate on crop types that can contribute the most to energy supplies while minimizing the impact of producing energy from crops on food production and the environment. Research in the HEC Program will focus on crops suitable for marginal croplands and on winter crops that can be grown between plantings of conventional crops. 1 table.

  8. Evidence of cryptic introgression in tomato (Solanum lycopersicum L.) based on wild tomato species alleles.

    Science.gov (United States)

    Labate, Joanne A; Robertson, Larry D

    2012-08-07

    Many highly beneficial traits (e.g. disease or abiotic stress resistance) have been transferred into crops through crosses with their wild relatives. The 13 recognized species of tomato (Solanum section Lycopersicon) are closely related to each other and wild species genes have been extensively used for improvement of the crop, Solanum lycopersicum L. In addition, the lack of geographical barriers has permitted natural hybridization between S. lycopersicum and its closest wild relative Solanum pimpinellifolium in Ecuador, Peru and northern Chile. In order to better understand patterns of S. lycopersicum diversity, we sequenced 47 markers ranging in length from 130 to 1200 bp (total of 24 kb) in genotypes of S. lycopersicum and wild tomato species S. pimpinellifolium, Solanum arcanum, Solanum peruvianum, Solanum pennellii and Solanum habrochaites. Between six and twelve genotypes were comparatively analyzed per marker. Several of the markers had previously been hypothesized as carrying wild species alleles within S. lycopersicum, i.e., cryptic introgressions. Each marker was mapped with high confidence (etomato whole genome shotgun chromosomes (SL2.40) database. Neighbor-joining trees showed high mean bootstrap support (86.8 ± 2.34%) for distinguishing red-fruited from green-fruited taxa for 38 of the markers. Hybridization and parsimony splits networks, genomic map positions of markers relative to documented introgressions, and historical origins of accessions were used to interpret evolutionary patterns at nine markers with putatively introgressed alleles. Of the 47 genetic markers surveyed in this study, four were involved in linkage drag on chromosome 9 during introgression breeding, while alleles at five markers apparently originated from natural hybridization with S. pimpinellifolium and were associated with primitive genotypes of S. lycopersicum. The positive identification of introgressed genes within crop species such as S. lycopersicum will help

  9. NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato

    KAUST Repository

    Thirumalaikumar, Venkatesh P.

    2017-06-22

    Water deficit (drought stress) massively restricts plant growth and the yield of crops; reducing the deleterious effects of drought is therefore of high agricultural relevance. Drought triggers diverse cellular processes including the inhibition of photosynthesis, the accumulation of cell-damaging reactive oxygen species, and gene expression reprogramming, besides others. Transcription factors (TF) are central regulators of transcriptional reprogramming and expression of many TF genes is affected by drought, including members of the NAC family. Here, we identify the NAC factor JUNGBRUNNEN1 (JUB1) as a regulator of drought tolerance in tomato (Solanum lycopersicum). Expression of tomato JUB1 (SlJUB1) is enhanced by various abiotic stresses, including drought. Inhibiting SlJUB1 by virus-induced gene silencing drastically lowers drought tolerance concomitant with an increase in ion leakage, an elevation of hydrogen peroxide (H2 O2 ) levels, and a decrease of the expression of various drought-responsive genes. In contrast, overexpression of AtJUB1 from Arabidopsis thaliana increases drought tolerance in tomato, alongside with a higher relative leaf water content during drought and reduced H2 O2 levels. AtJUB1 was previously shown to stimulate expression of DREB2A, a TF involved in drought responses, and of the DELLA genes GAI and RGL1. We show here that SlJUB1 similarly controls the expression of the tomato orthologs SlDREB1, SlDREB2, and SlDELLA. Furthermore, AtJUB1 directly binds to the promoters of SlDREB1, SlDREB2 and SlDELLA in tomato. Our study highlights JUB1 as a transcriptional regulator of drought tolerance and suggests considerable conservation of the abiotic stress-related gene regulatory networks controlled by this NAC factor between Arabidopsis and tomato. This article is protected by copyright. All rights reserved.

  10. Crop candidates for the bioregenerative life support systems in China

    Science.gov (United States)

    Chunxiao, Xu; Hong, Liu

    The use of plants for life support applications in space is appealing because of the multiple life support functions by the plants. Research on crops that were grown in the life support system to provide food and oxygen, remove carbon dioxide was begun from 1960. To select possible crops for research on the bioregenerative life support systems in China, criteria for the selection of potential crops were made, and selection of crops was carried out based on these criteria. The results showed that 14 crops including 4 food crops (wheat, rice, soybean and peanut) and 7 vegetables (Chinese cabbage, lettuce, radish, carrot, tomato, squash and pepper) won higher scores. Wheat ( Triticum aestivum L.), rice ( Oryza sativa L.), soybean ( Glycine max L.) and peanut ( Arachis hypogaea L.) are main food crops in China. Chinese cabbage ( Brassica campestris L. ssp. chinensis var. communis), lettuce ( Lactuca sativa L. var. longifolia Lam.), radish ( Raphanus sativus L.), carrot ( Daucus carota L. var. sativa DC.), tomato ( Lycopersicon escalentum L.), squash ( Cucurbita moschata Duch.) and pepper ( Capsicum frutescens L. var. longum Bailey) are 7 vegetables preferred by Chinese. Furthermore, coriander ( Coriandum sativum L.), welsh onion ( Allium fistulosum L. var. giganteum Makino) and garlic ( Allium sativum L.) were selected as condiments to improve the taste of space crew. To each crop species, several cultivars were selected for further research according to their agronomic characteristics.

  11. Impact of integrated pest management on the population of leafminers, fruit borers, and natural enemies in tomato

    OpenAIRE

    2005-01-01

    The objective of this work was to evaluate the impact of integrated pest management (IPM) in the productivity of the tomato and in the populations of leafminers, fruit borers, and natural enemies in tomato crops. The treatments were calendar (spraying twice weekly with insecticides and fungicides), IPM (spraying when action thresholds were achieved), and control (no pesticide was applied). IPM was the most efficient system of pest control due to presenting similar productivity and 65.6% less ...

  12. Different chromatin fractions of tomato (Solanum lycopersicum L.) and related species.

    Science.gov (United States)

    Brasileiro-Vidal, A C; Melo-Oliveira, M B; Carvalheira, G M G; Guerra, M

    2009-12-01

    Conventional chromosome staining has suggested that more than 75% of the tomato chromosomes are constituted by heterochromatin. In order to determine whether more deeply stained proximal regions are classic heterochromatin, the distributions of C-bands and chromomycin A(3) (CMA) bands, and the prophase condensation patterns, were analysed in tomato. In this and most other species of the tomato clade, the 5S and 45S rDNA sites were also localised. In tomato, CMA banding was similar to C-banding. After conventional staining, all species displayed large condensed heteropycnotic regions that did not correspond to C-bands or CMA bands. Analyses of the CMA banded karyotypes revealed a low heterochromatin content. Around 12-17% of the chromatin of tomato was CMA(+) and 1/4 to 1/5 of this heterochromatin corresponded to 45S rDNA. In other species, the CMA(+) heterochromatin showed extensive variation (8-35%), but was never near the values found in the literature for tomato. These data suggest the existence of three principal fractions of chromatin in tomato and related species: the late condensed euchromatin corresponding to the terminal regions of the chromosomes, the precocious condensed euchromatin that occupies the major part of the chromosomes and the constitutive heterochromatin that represents those regions revealed by C-bands.

  13. Transgenic tomato hybrids resistant to tomato spotted wilt virus infection.

    NARCIS (Netherlands)

    Haan, de P.; Ultzen, T.; Prins, M.; Gielen, J.; Goldbach, R.; Grinsven, van M.

    1996-01-01

    Tomato spotted wilt virus (TSWV) infections cause significant economic losses in the commercial culture of tomato (Lycopersicon esculentum). Culture practices have only been marginally effective in controlling TSWV. The ultimate way to minimize losses caused by TSWV is resistant varieties. These can

  14. Transgenic tomato hybrids resistant to tomato spotted wilt virus infection.

    NARCIS (Netherlands)

    Haan, de P.; Ultzen, T.; Prins, M.; Gielen, J.; Goldbach, R.; Grinsven, van M.

    1996-01-01

    Tomato spotted wilt virus (TSWV) infections cause significant economic losses in the commercial culture of tomato (Lycopersicon esculentum). Culture practices have only been marginally effective in controlling TSWV. The ultimate way to minimize losses caused by TSWV is resistant varieties. These can

  15. 21 CFR 73.585 - Tomato lycopene extract; tomato lycopene concentrate.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Tomato lycopene extract; tomato lycopene... SERVICES GENERAL LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.585 Tomato lycopene extract; tomato lycopene concentrate. (a) Identity. (1) The color additive tomato lycopene extract is...

  16. Incidence of Phytophthora infestans (Mont.) de Bary on potato and tomato in Maine, 2006-2010

    Science.gov (United States)

    Late blight, caused by Phytophthora infestans, is a devastating disease globally. In Maine, we recorded late blight on potato and tomato during the 2006-2010 cropping seasons. From 2006 to 2008, over 90% of diseased samples were collected in potato fields from northern and central Aroostook County i...

  17. Virus-induced gene silencing in detached tomatoes and biochemical effects of phytoene desaturase gene silencing

    NARCIS (Netherlands)

    Romero, I.; Tikunov, Y.M.; Bovy, A.G.

    2011-01-01

    Virus-induced gene silencing (VIGS) is a technology that has rapidly emerged for gene function studies in plants. Many advances have been made in applying this technique in an increasing number of crops. Recently, VIGS has been successfully used to silence genes in tomato fruit through agroinfiltrat

  18. Salicylic acid-mediated elicitation of tomato defense against infection by potato purple top phytoplasma

    Science.gov (United States)

    Recent outbreaks and continued spread of phytoplasma infection-associated diseases in potato, tomato, and other vegetable crops in the U.S. accentuates the need for practical strategies to mitigate the impact of the phytoplasmal diseases. The purpose of the current study was to investigate whether ...

  19. Biological control of whitefly on greenhouse tomato in Colombia: Encarsia formosa or Amitus fuscipennis?

    NARCIS (Netherlands)

    Vis, de R.J.

    2001-01-01

    In Colombia, biological control of pests in greenhouse crops is only applied on a very limited scale in ornamentals and as yet non-existent in greenhouse vegetables. Greenhouse production of vegetables - mostly tomatoes- is a recent development, as a result of the high losses of field production due

  20. Tomato fruit growth : integrating cell division, cell growth and endoreduplication by experimentation and modelling

    NARCIS (Netherlands)

    Fanwoua, J.

    2012-01-01

    Keywords: cell division, cell growth, cell endoreduplication, fruit growth, genotype, G×E interaction, model, tomato. Fruit size is a major component of fruit yield and quality of many crops. Variations in fruit size can be tremendous due to genotypic and environmental factors. The mechanisms

  1. Automatic Weed Control System For Transplanted Processing Tomatoes Using X-ray Stem Sensing

    Science.gov (United States)

    A stem detection system was developed for automatic weed control in transplanted tomato fields. A portable x-ray source projected an x-ray beam perpendicular to the crop row and parallel to the soil surface. The plant’s main stem absorbs x-ray energy, decreasing the detected signal and allowing ste...

  2. Metabolites, their decomposition, production of tomato and bioassays from open and closed rockwool systems

    NARCIS (Netherlands)

    Kreij, de C.; Runia, W.T.; Burg, van der A.M.M.

    2004-01-01

    Growth, decrease in yield and other, undefined problems are reported to be due to the recirculation of the nutrient solution, which is compulsory for crops grown on substrates in the Netherlands. In a trial with tomato grown on rockwool, open and closed systems were compared. Drain water from both s

  3. First report of the root-knot nematode Meloidogyne ethiopica on tomato in Slovenia

    NARCIS (Netherlands)

    Sirca, S.; Urek, G.; Karssen, G.

    2004-01-01

    The root-knot nematode Meloidogyne ethiopica Whitehead originally described from Tanzania is also distributed in South Africa, Zimbabwe, and Ethiopia (3). Although this species is a relatively unknown root-knot nematode, M. ethiopica parasitizes several economical important crops, such as tomato, co

  4. First report of the natural occurrence of tomato chlorotic spot virus in peanuts in Haiti

    Science.gov (United States)

    Tomato chlorotic spot virus (TCSV) was identified in peanut in Haiti. This is the first report of TCSV naturally infecting peanut. Genetic diversity of TCSV was characterized. This report provides an overview of this emerging virus for growers, extension workers, crop consultants and research and...

  5. X-ray based stem detection in an automatic tomato weeding system

    Science.gov (United States)

    A stem detection system was developed for automatic weed control in transplanted tomato fields. A portable x-ray source projected an x-ray beam perpendicular to the crop row and parallel to the soil surface. The plant’s main stem absorbs x-ray energy, decreasing the detected signal and allowing stem...

  6. X-ray based stem detection in an automated tomato weeding system

    Science.gov (United States)

    A stem detection system was developed for automatic weed control in transplanted tomato fields. A portable x-ray source projected an x-ray beam perpendicular to the crop row and parallel to the soil surface. The plant’s main stem absorbs x-ray energy, decreasing the detected signal and allowing stem...

  7. Evaluation of thrips resistance in pepper to control Tomato spotted wilt virus infection

    NARCIS (Netherlands)

    Maris, P.C.

    2004-01-01

    The objective of this study was to analyse the effect of thrips ( F. occidentalis ) resistance in pepper ( Capsicum ) on the spread of Tomato spotted wilt virus (TSWV). Results demonstrate that the rate of primary TSWV-infection is effectively limited in a thrips-resistant (TR) pepper crop compared

  8. Evaluation of bio-agent formulations to control Fusarium wilt of tomato

    African Journals Online (AJOL)

    ONOS

    2010-09-06

    Sep 6, 2010 ... All tested bio-agent formulations could significantly increase the yield of tomato ... is one of the world's most cultivated vegetable crop. Fusarium wilt is .... 0.2 g of fresh weight mycelium was ground .... temperature for 5 days; then colony margin was cut by 3 mm diameter ... shelf life at every month for 1 year.

  9. Identification of genes affecting the response of tomato and Arabidopsis upon powdery mildew infection

    NARCIS (Netherlands)

    Gao, D.

    2014-01-01

      Many plant species are hosts of powdery mildew fungi, including Arabidopsis and economically important crops such as wheat, barley and tomato. Resistance has been explored using induced mutagenesis and natural variation in the plant species. The isolated genes encompass loss-of-function susc

  10. Towards isolation of the tomato root-knot nematode resistance gene Mi via positional cloning.

    NARCIS (Netherlands)

    Daelen, van R.A.J.J.

    1995-01-01

    Root-knot nematodes of the genus Meloidogyne are severe pathogens of plants and worldwide they cause damage to many economically important crops like potato, rice, cotton, and tomato. So the control of nematodes and the protection of plants against nematode damage are matters of major concern. Some

  11. Traps and trap placement may affect location of brown marmorated stink bug (Hemiptera: Pentatomidae) and increase injury to tomato fruits in home gardens.

    Science.gov (United States)

    Sargent, Chris; Martinson, Holly M; Raupp, Michael J

    2014-04-01

    The invasive brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), is an important pest of field crops, fruit orchards, commercial vegetables, ornamental plants, and home vegetable gardens. Pheromone-baited traps designed to attract, trap, and kill H. halys are marketed for use in home gardens to reduce damage to plants. To test this assertion, we conducted the following experiment: One group of 15 gardeners placed stink bug traps at the end of a row of tomatoes, Solanum lycopersicum (L.), in their vegetable garden and another group of 14 placed no traps in their garden and served as controls. Gardeners with traps were no more or less likely to have H. halys on tomato plants than those without traps, but the abundance of H. halys on tomato fruits was marginally greater in gardens with traps. However, tomato fruits grown in gardens with traps sustained significantly more injury than tomato fruits grown in gardens without traps. Furthermore, tomato fruits on plants near the trap housed more H. halys than tomato fruits on plants at the end of a row away from the trap. Traps may be useful in identifying gardens where H. halys is likely to be found and ones in which stink bug injury to tomatoes is likely. We found no evidence that stink bug traps protected tomatoes from H. halys, and it appears that the addition of traps to gardens may increase injury to tomato fruits.

  12. Bacterial canker resistance in tomato

    NARCIS (Netherlands)

    Sen, Y.

    2014-01-01

    Clavibacter michiganensis subsp. michiganensis (Cmm) is the pathogen causing bacterial  canker in tomato. The disease was described for the first time in 1910 in Michigan, USA. Cmmis considered the most harmful bacteria threatening tomato. Disease transmission occurs via seed and symptoms becom

  13. 21 CFR 155.190 - Canned tomatoes.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Canned tomatoes. 155.190 Section 155.190 Food and... tomatoes. (a) Identity—(1) Description. (i) Canned tomatoes is the food prepared from mature tomatoes conforming to the characteristics of the fruit Lycopersicum esculentum P. Mill, of red or reddish...

  14. Obtaining of Grafted Planting Material at Some Romanian Tomatoes

    Directory of Open Access Journals (Sweden)

    Madalina Doltu

    2016-11-01

    Full Text Available The tomatoes have highest share in Romanian crops from protected spaces (greenhouses, solariums. The grafting is an agronomical technique that induces or improves some qualities of the tomato cultivars (resistance to soil diseases and pests, resistance to abiotic factors, quantity and quality of fruit production. The research was aimed the establishing of the technological stages for producing of scion and rootstock seedlings from L. esculentum species, to obtain compatible phenotype when is grafted. The observations of this research were conducted on Department of Horticultural Cultures in Protected Spaces from Horting Institute Bucharest. The experience was carry out on a cultivar collection consisting from L. esculentum plants: scions (‘Siriana’–F1 hybrid and ‘Buzău 1600’– variety, creations from the germplasm bank of Research and Development Station for Vegetable Growing Buzău Romania (VDRS Buzău and rootstock (‘Groundforce’–F1 hybrid. The plant diameters were correlated for a grafting by the annexation method, cutting at 45 degrees. The grafting was performed successfully. The technological steps have achieved phenotypic compatibility of the symbiotes when was the grafting by annexation. The technology for producing of scion and rootstock seedlings at these Romanian tomatoes (‘Siriana’ and ‘Buzău’ 1600 was established for the crops in protected spaces in south area of Romania.

  15. Biological agents for whitefly control in Sardinian greenhouse tomatoes.

    Science.gov (United States)

    Nannini, M; Foddi, F; Manca, L; Pisci, R; Sanna, F

    2009-01-01

    To evaluate the effectiveness of alternative options for biocontrol of whiteflies in greenhouse tomatoes, an experiment was carried out during the cropping season 2005-2006 in one of Sardinia's major horticultural districts (S. Margherita di Pula, Cagliari, Italy). Twelve long-cycle and 17 short-cycle tomato crops (8 autumn and 9 spring crops) were surveyed. All of them were treated for insect pest control at the beginning of the growing season, but in 19 out of 29 cases whitefly natural enemies were also released (BCA greenhouses), at least four weeks after the last treatment. The following release programmes were tested: on autumn crops, 1 Macrolophus caliginosus and 12 Eretmocerus mundus/m2; on long-cycle crops, 1 M. caliginosus (released in autumn or spring) and 24 Encarsia formosa/m2 or 48 E. formosa/m2; on spring crops, 1 M. caliginosus and 24 E. formosa/m2 or 48 E. formosa/m2. The cost of each option was fixed at approximately 0.25 Euros/m2. The remaining greenhouses were maintained as controls (no BCA greenhouses). While whitefly and mirid populations were monitored monthly, whitefly species composition and mortality of immature stages were estimated at least twice during the growing season. On short-cycle autumn crops, the release of M. caliginosus and E. mundus produced negligible results in terms of Bemisia tabaci control. On long-cycle and spring crops, even though in June mortality rates in BCA greenhouses were found to be 2- to 3-fold higher than in no-BCA greenhouses, Trialeurodes vaporariorum population growth was not significantly affected by natural enemies. Among the beneficials tested, E. formosa proved to be the most effective; E. mundus and M. caliginosus did not establish well, probably owing to the persistence of insecticide residues, scarce prey availability and intense plant de-leafing. The presence of indigenous natural enemies of whiteflies was observed in most sites, but in general they contributed little to biological control. The

  16. First report of tomato chlorotic spot virus in non-solanaceous weeds erect spiderling (Boerhavia erecta) and asian spiderflower (Cleome viscosa), and sweet chili pepper (Capsicum chinense) in Puerto Rico

    Science.gov (United States)

    Tomato chlorotic spot virus (TCSV) has recently been detected in tomato, bell pepper, jimsonweed and lettuce in Puerto Rico. Observations of weeds and additional crops in 2015 and 2016 revealed TCSV-like symptoms. Testing of these symptomatic plants identified three new hosts of TCSV in Puerto Ric...

  17. Plant senescence and crop productivity

    DEFF Research Database (Denmark)

    Gregersen, Per L.; Culetic, Andrea; Boschian, Luca

    2013-01-01

    Senescence is a developmental process which in annual crop plants overlaps with the reproductive phase. Senescence might reduce crop yield when it is induced prematurely under adverse environmental conditions. This review covers the role of senescence for the productivity of crop plants....... With the aim to enhance productivity, a number of functional stay-green cultivars have been selected by conventional breeding, in particular of sorghum and maize. In many cases, a positive correlation between leaf area duration and yield has been observed, although in a number of other cases, stay...... plants, the expression of the IPT gene under control of senescence-associated promoters has been the most successful. The promoters employed for senescence-regulated expression contain cis-elements for binding of WRKY transcription factors and factors controlled by abscisic acid. In most crops...

  18. EFFECT OF SEAWEED EXTRACT OF SARGASSUM VULGARE ON GERMINATION BEHAVIOR OF TWO TOMATOES CULTIVARS (SOLANUM LYCOPERSICUM L UNDER SALT STRESS

    Directory of Open Access Journals (Sweden)

    Elouaer Mohamed Aymen

    2014-09-01

    Full Text Available Salt stress is a major adverse factor that can lower seed germination and seedlings growth, leading to reduced plant growth and ultimately lower crop productivity in arid and semi arid regions of the world. Tomato (Solanum lycopersicum L. is an important crop occupying a large area in both Morocco and Tunisia, where salt stress is the most limiting factor. In the present investigation, an attempt has been made to study the effect of seaweed liquid extract (SWE from Sargassum vulgare at different concentrations on seed germination and seedling growth of two tomatoes cultivars: cv Agatha and cv Nemadore under salt stress.

  19. Optimizing chemically induced resistance in tomato against Botrytis cinerea

    DEFF Research Database (Denmark)

    Luna, Estrella; Beardon, Emily G; Ravnskov, Sabine

    2016-01-01

    Resistance-inducing chemicals can offer broad-spectrum disease protection in crops, but can also affect plant growth and interactions with plant-beneficial microbes. We have evaluated different application methods of ß-aminobutyric acid (BABA) and jasmonic acid (JA) for long-lasting induced...... resistance in tomato against Botrytis cinerea. In addition, we have studied non-target effects on plant growth and root colonization by arbuscular mycorrhizal fungi (AMF). Germinating seeds for one week in BABA- or JA-containing solutions promoted seed germination efficiency, did not affect plant growth...... repressed plant growth at higher concentrations of the chemicals, which was particularly pronounced in hydroponically grown plants after BABA treatment. Both seed coating with BABA, and seedling treatments with BABA or JA, did not affect AMF root colonization in soil-grown tomato. Our study has identified...

  20. Tomato bushy stunt virus (TBSV) infecting Lycopersicon esculentum.

    Science.gov (United States)

    Hafez, El Sayed E; Saber, Ghada A; Fattouh, Faiza A

    2010-01-01

    Tomato bushy stunt virus (TBSV) was detected in tomato crop (Lycopersicon esculentum) in Egypt with characteristic mosaic leaf deformation, stunting, and bushy growth symptoms. TBSV infection was confirmed serologically by ELISA and calculated incidence was 25.5%. Basic physicochemical properties of a purified TBSV Egh isolate were identical to known properties of tombusviruses of isometric 30-nm diameter particles, 41-kDa coat protein and the genome of approximately 4800 nt. This is the first TBSV isolate reported in Egypt. Cloning and partial sequencing of the isolate showed that it is more closely related to TBSV-P and TBSV-Ch than TBSV-Nf and TBSV-S strains of the virus. However, it is distinct from the above strains and could be a new strain of the virus which further confirms the genetic diversity of tombusviruses.

  1. Organic fertigation for greenhouse crops

    DEFF Research Database (Denmark)

    Pokhrel, Bhaniswor

    2017-01-01

    productivity is suboptimal nutrient management resulting from poor synchronization between crop nutrient demand and nutrient release from organic fertilizers, affecting the physical, chemical and biological characteristics of the root zone environment, and thus plant growth and productivity. Compared to solid...... of acidic water with ammonia. These fertilizers and commercially available lupin sap as well as pH-controlled chicken manure extract were applied either alone or in combinations to tomato, parsley or coriander grown in a peat-based medium. Their effect on nutrient availability, pH, electrical conductivity...... organic fertilizers, the application of liquid organic fertilizers potentially more accurately addresses the nutrient demand, because nutrients are readily available and different fertilizers are easily mixed. This PhD work explores the possibilities and challenges related to the application of liquid...

  2. Automatic image cropping for republishing

    Science.gov (United States)

    Cheatle, Phil

    2010-02-01

    Image cropping is an important aspect of creating aesthetically pleasing web pages and repurposing content for different web or printed output layouts. Cropping provides both the possibility of improving the composition of the image, and also the ability to change the aspect ratio of the image to suit the layout design needs of different document or web page formats. This paper presents a method for aesthetically cropping images on the basis of their content. Underlying the approach is a novel segmentation-based saliency method which identifies some regions as "distractions", as an alternative to the conventional "foreground" and "background" classifications. Distractions are a particular problem with typical consumer photos found on social networking websites such as FaceBook, Flickr etc. Automatic cropping is achieved by identifying the main subject area of the image and then using an optimization search to expand this to form an aesthetically pleasing crop. Evaluation of aesthetic functions like auto-crop is difficult as there is no single correct solution. A further contribution of this paper is an automated evaluation method which goes some way towards handling the complexity of aesthetic assessment. This allows crop algorithms to be easily evaluated against a large test set.

  3. Effects of Different Preceding Crops on Soil Micro-ecological Environment and Yield of Cucumber

    Institute of Scientific and Technical Information of China (English)

    LIU Shouwei; LIU Shuqin; PAN Kai; WANG Lili; WU Fengzhi

    2011-01-01

    Pepper, celery, eggplant and tomato were used as preceding crops to study their effects on the yield, soil microorganism quantity and soil enzyme activities of cucumber. Results showed that four preceding crops all increased soil microorganism quantity in cucumber, but decreased population of Fusarium oxysporum. The effect of pepper was more significant than that of the others Populations of soil bacteria, fungi and actinomyces of pepper treatment were significantly higher than those of the other treatments, except that the populations of fungi had no significant difference with celery and eggplant treatments on 50 days after transplanting, while that of Fusarium oxysporum was fewer than that of the other treatments. The soil microorganism quantity in celery and eggplant treatment was more significant than tomato, but lower population of Fusarium oxysporum. Four preceding crops all increased sol enzyme activities, lnvertase and urease activities of pepper treatment were significantly higher than those of the other treatments, catalase activities of pepper, celery and eggplant treatments were significantly higher than those of tomato and the control treatments All preceding crops remarkably increased cucumber yield, with pepper as the highest. Comparing with the control, cucumber yields of pepper, celery and eggplant treatments increased by 24.9%, 13.6% and 11.9%, respectively. Results suggested that four preceding crops all improved soil microbial ecology and increased cucumber yield. The pepper was the most suitable preceding crop, then followed by celery and eggplant. Tomato had the similar effect as the control.

  4. Tomato Productivity and Quality in Aquaponics: Comparison of Three Hydroponic Methods

    Directory of Open Access Journals (Sweden)

    Zala Schmautz

    2016-11-01

    Full Text Available Aquaponics (AP is a food production system that combines hydroponic (HP crop production with recirculating aquaculture. Different types of hydroponic systems have been used for growing crops in aquaponics. However, very few studies have compared their suitability and efficiency in an aquaponic context. The study presented here compares tomato yield, morphological (external and biochemical (internal fruit quality, and overall tomato plant vitality from three different HP systems (nutrient film technique, drip irrigation system, and floating raft culture and examines the distribution of nutrients in different parts of the tomato plant. Three replicate AP systems were set up, each incorporating the three different HP systems coupled with a separate recirculating aquaculture unit growing Nile tilapia. The results showed that the choice of the cultivation system had little influence on most of the above-mentioned properties. Tomato fruit mineral content was found to be in similar range for N, P, K, Ca, Mg, Fe, and Zn as reported in the literature. Yield and fruit quality were similar in all three systems. However, the drip irrigation system did perform slightly better. The slightly higher oxygen radical absorbance capacity (ORAC of the fruits grown in AP in comparison to commercially produced and supermarket derived tomatoes might indicate a potential for producing fruits with higher health value for humans.

  5. Water Footprint of Industrial Tomato Cultivations in the Pinios River Basin: Soil Properties Interactions

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    Eleftherios Evangelou

    2016-11-01

    Full Text Available Industrial tomatoes are cultivated in about 4000 ha of the Pinios river basin (central Greece, providing significant income to the farmers. In this study, the water footprint (WF of industrial tomatoes between planting and harvest was estimated in 24 different farms for three consecutive years. The selected farms were representative of the main agro-climatic zones and soil textural classes within the river basin. Green, blue and grey WF calculations were based on datasets of the experimental plots for each farm, including irrigation water volume, meteorological, soil, and crop yield data. The results showed that the WF of tomatoes ranged from 37 to 131 m3 water/ton tomatoes with an average of 61 m3/ton. The WF variation depended mainly on crop yield, local agro-climatic and soil conditions. The green, blue, and grey WF components averaged 13, 27 and 21 m3/ton, respectively. The results reveal the importance of WF in understanding how tomato production relates to the sustainable use of freshwater and pollution at local level.

  6. Responses of grafted tomato (Solanum lycopersiocon L. to abiotic stresses in Saudi Arabia

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    Abdulaziz Al-Harbi

    2017-09-01

    Full Text Available Quantity and quality of irrigation water are considered the most imperative limiting factors for plant production in arid environment. Adoptions of strategies can minimize crop water consumption while nonexistent yield reduction is considered challenge for scholars especially in arid environment. Grafting is regarded as a promising tool to avoid or reduce yield loss caused by abiotic stresses. Tomato (Solanum lycopersium Mill., commercial cultivar Faridah was grafted on Unifort rootstock and grown under regulated deficit irrigation (RDI (100%, 80% and 60% ETc, using two types of irrigation water, fresh (EC = 0.86 dS/m and brackish (EC = 3.52 dS/m. The effects of grafting and RDI on water use efficiency, vegetative growth, yield, fruit quality were investigated. Plant vegetative growth was reduced under water and salinity stresses. Grafting the plant significantly improves the vegetative growth under both conditions. The results showed that crop yield, Ca+2 and K+ were considerably increased in grafted tomato compared to non-grafted plants under water and salinity stresses. Grafted tomato plants accumulated less Na+ and Cl−, especially under high levels of salinity compared to non-grafted plants. Grafting tomato plants showed a slight decrease on the fruit quality traits such as vitamin C, titratable acidity (TA and total soluble solids (TSS. This study confirmed that grafted tomato plants can mitigate undesirable impact of salt stress on growth and fruit quality.

  7. Molecular Identification of Transgenic Tomato in Iran by P35S PromoterBased PCR

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    Parastoo Khanmohammad

    2016-06-01

    Full Text Available Background: With the increasing population of the world, we should face food shortages in the near future. In this regard, agriculture of transgenic crops becomes very important. However, ensuring of GM products labeling is of great importance. Tomato is one of the most important food crops and for this reason it has undergone many genetic changes. This study aims to introduce a rapid, sensitive, and accurate method to identify non-labeled transgenic tomatoes in Iran market. Results: In this study, after optimization of PCR test based on P35S promoter, the amplicon was cloned in the plasmid PTZ57R in Escherichia coli JM107 to confirm and make positive control. After optimized PCR test of 50 tomatoes it was found that 4% of the Iranian markets’ tomatoes contain P35S promoter and are probably transgenic while none of the samples have been labeled in this regard. Conclusion: PCR technique is a suitable, available, fast and accurate method for screening transgenic products such as tomatoes.

  8. High-Throughput Genomics Enhances Tomato Breeding Efficiency

    Science.gov (United States)

    Barone, A; Di Matteo, A; Carputo, D; Frusciante, L

    2009-01-01

    Tomato (Solanum lycopersicum) is considered a model plant species for a group of economically important crops, such as potato, pepper, eggplant, since it exhibits a reduced genomic size (950 Mb), a short generation time, and routine transformation technologies. Moreover, it shares with the other Solanaceous plants the same haploid chromosome number and a high level of conserved genomic organization. Finally, many genomic and genetic resources are actually available for tomato, and the sequencing of its genome is in progress. These features make tomato an ideal species for theoretical studies and practical applications in the genomics field. The present review describes how structural genomics assist the selection of new varieties resistant to pathogens that cause damage to this crop. Many molecular markers highly linked to resistance genes and cloned resistance genes are available and could be used for a high-throughput screening of multiresistant varieties. Moreover, a new genomics-assisted breeding approach for improving fruit quality is presented and discussed. It relies on the identification of genetic mechanisms controlling the trait of interest through functional genomics tools. Following this approach, polymorphisms in major gene sequences responsible for variability in the expression of the trait under study are then exploited for tracking simultaneously favourable allele combinations in breeding programs using high-throughput genomic technologies. This aims at pyramiding in the genetic background of commercial cultivars alleles that increase their performances. In conclusion, tomato breeding strategies supported by advanced technologies are expected to target increased productivity and lower costs of improved genotypes even for complex traits. PMID:19721805

  9. Automatic detection and segmentation of stems of potted tomato plant using Kinect

    Science.gov (United States)

    Fu, Daichang; Xu, Lihong; Li, Dawei; Xin, Longjiao

    2014-04-01

    The automatic segmentation and recognition of greenhouse crop is an important aspect in digitized facility agriculture. Crop stems are closely related with the growth of the crop. Meanwhile, they are also an important physiological trait to identify the species of plants. For these reasons, this paper focuses on the digitization process to collect and analysis stems of greenhouse plants (tomatoes). An algorithm for automatic stem detection and extraction is proposed, based on a cheap and effective stereo vision system—Kinect. In order to demonstrate the usefulness and the potential applicability of our algorithm, a virtual tomato plant, whose stems are rendered by segmented stem texture samples, is reconstructed on OpenGL graphic platform.

  10. Epidemiology and genetic diversity of criniviruses associated with tomato yellows disease in Greece.

    Science.gov (United States)

    Orfanidou, C G; Dimitriou, C; Papayiannis, L C; Maliogka, V I; Katis, N I

    2014-06-24

    Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV) are two whitefly transmitted viruses which are classified in the genus Crinivirus of the family Closteroviridae. Both induce similar yellowing symptoms in tomato and are responsible for severe economic losses. ToCV is transmitted by Bemisia tabaci Gennadious, Trialeurodes vaporariorum Westwood and Trialeurodes abutilonea Haldeman, whereas TICV is transmitted only by T. vaporariorum. An extensive study was conducted during 2009-2012 in order to identify the virus species involved in tomato yellowing disease in Greece. Samples from tomato, other crops and weeds belonging to 44 species from 26 families were collected and analyzed using molecular methods. In addition, adult whiteflies were collected and analyzed using morphological characters and DNA markers. Results showed that TICV prevailed in tomato crops (62.5%), while ToCV incidence was lower (20.5%) and confined in southern Greece. ToCV was also detected in lettuce plants showing mild yellowing symptoms for the first time in Greece. Approximately 13% of the tested weeds were found to be infected, with TICV being the predominant virus with an incidence of 10.8%, whereas ToCV was detected only in 2.2% of the analyzed samples. These results indicate that the host range of TICV and ToCV in Greece is far more extensive than previously believed. T. vaporariorum was the most widespread whitefly species in Greece (80%), followed by B. tabaci (biotypes B and Q) (20%). Sequence analysis of the CP and CPm genes from Greek tomato and weed isolates of ToCV and TICV showed that even though both viruses have very wide host ranges their populations show very low molecular divergence.

  11. Bacterial community diversity and variation in spray water sources and the tomato fruit surface

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    Ottesen Andrea R

    2011-04-01

    Full Text Available Abstract Background Tomato (Solanum lycopersicum consumption has been one of the most common causes of produce-associated salmonellosis in the United States. Contamination may originate from animal waste, insects, soil or water. Current guidelines for fresh tomato production recommend the use of potable water for applications coming in direct contact with the fruit, but due to high demand, water from other sources is frequently used. We sought to describe the overall bacterial diversity on the surface of tomato fruit and the effect of two different water sources (ground and surface water when used for direct crop applications by generating a 454-pyrosequencing 16S rRNA dataset of these different environments. This study represents the first in depth characterization of bacterial communities in the tomato fruit surface and the water sources commonly used in commercial vegetable production. Results The two water sources tested had a significantly different bacterial composition. Proteobacteria was predominant in groundwater samples, whereas in the significantly more diverse surface water, abundant phyla also included Firmicutes, Actinobacteria and Verrucomicrobia. The fruit surface bacterial communities on tomatoes sprayed with both water sources could not be differentiated using various statistical methods. Both fruit surface environments had a high representation of Gammaproteobacteria, and within this class the genera Pantoea and Enterobacter were the most abundant. Conclusions Despite the major differences observed in the bacterial composition of ground and surface water, the season long use of these very different water sources did not have a significant impact on the bacterial composition of the tomato fruit surface. This study has provided the first next-generation sequencing database describing the bacterial communities living in the fruit surface of a tomato crop under two different spray water regimes, and therefore represents an

  12. Tomato yellow leaf curl viruses: ménage à trois between the virus complex, the plant and the whitefly vector.

    Science.gov (United States)

    Díaz-Pendón, Juan Antonio; Cañizares, M Carmen; Moriones, Enrique; Bejarano, Eduardo R; Czosnek, Henryk; Navas-Castillo, Jesús

    2010-07-01

    Tomato yellow leaf curl disease (TYLCD) is one of the most devastating viral diseases affecting tomato crops in tropical, subtropical and temperate regions of the world. Here, we focus on the interactions through recombination between the different begomovirus species causing TYLCD, provide an overview of the interactions with the cellular genes involved in viral replication, and highlight recent progress on the relationships between these viruses and their vector, the whitefly Bemisia tabaci. The tomato yellow leaf curl virus-like viruses (TYLCVs) are a complex of begomoviruses (family Geminiviridae, genus Begomovirus) including 10 accepted species: Tomato yellow leaf curl Axarquia virus (TYLCAxV), Tomato yellow leaf curl China virus (TYLCCNV), Tomato yellow leaf curl Guangdong virus (TYLCGuV), Tomato yellow leaf curl Indonesia virus (TYLCIDV), Tomato yellow leaf curl Kanchanaburi virus (TYLVKaV), Tomato yellow leaf curl Malaga virus (TYLCMalV), Tomato yellow leaf curl Mali virus (TYLCMLV), Tomato yellow leaf curl Sardinia virus (TYLCSV), Tomato yellow leaf curl Thailand virus (TYLCTHV), Tomato yellow leaf curl Vietnam virus (TYLCVNV) and Tomato yellow leaf curl virus(TYLCV). We follow the species demarcation criteria of the International Committee on Taxonomy of Viruses (ICTV), the most important of which is an 89% nucleotide identity threshold between full-length DNA-A component nucleotide sequences for begomovirus species. Strains of a species are defined by a 93% nucleotide identity threshold. The primary host of TYLCVs is tomato (Solanum lycopersicum), but they can also naturally infect other crops [common bean (Phaseolus vulgaris), sweet pepper (Capsicum annuum), chilli pepper (C. chinense) and tobacco (Nicotiana tabacum)], a number of ornamentals [petunia (Petuniaxhybrida) and lisianthus (Eustoma grandiflora)], as well as common weeds (Solanum nigrum and Datura stramonium). TYLCVs also infect the experimental host Nicotiana benthamiana. Infected tomato

  13. Relationship between Tomato yellow leaf curl viruses and the whitefly vector.

    Science.gov (United States)

    Ssekyewa, Charles; Van Damme, Patrick L J; Hofte, Monica

    2007-01-01

    Tomato Yellow Leaf Curl Virus (TYLCV) and Tomato Leaf Curl Virus (ToLCV) are the currently known begomoviruses in Uganda. The relationship with their whiteflies (Bemisia tabaci) vector and its management were not known in Uganda. A direct relationship was expected between these begomoviruses and whiteflies at Buwama in Mpigi district of Uganda. Farmer practices were expected to have limited efficacy. To investigate this, a completely randomised block design was used for all trials with six treatments replicated three times. Treatments evaluated were farmer whitefly pest management practices. These included both chemical pesticide and non-pesticide applications. Data on whitefly population and tomato yellow leaf curl virus disease incidence was recorded weekly, and analysed using SAS and SPSS statistical programmes for ANOVA, and correlations. Ranked means, coefficients of variation and standard errors were noted. Virus-vector relationship field studies established that virus occurrence varied in space and time, and with management practices, crop development stage, and weather conditions. A negative relationship (R = -0.14, p 0.04) was established between number of plants infected with TYLCV (sensu lato) and percentage marketable tomato yield. Tomato maturity was inversely propotional to whitefly infestation (R = -0.5, p 0.0001). Uprooting and application of dimethoate was most effective of the six treatments. Tomato leaf curl virus diseases and whitefly management options were established in Uganda.

  14. Effects of Different Quantities of Tea-leaf Wormcast Substrate on the Growth of Tomato Seedling

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    YANG Wei

    2015-08-01

    Full Text Available The tomato seedling experiment was conducted to investigate the effect of substrate on the tomato seedling growth from five proportions of tea-leaf wormcast to peat, perlite and vermiculite, namely treatment I(1:5:2:2, treatment Ⅱ(2:4:2:2, treatment Ⅲ(3:3:2:2, treatment Ⅳ(4:2:2:2, treatment Ⅴ(6:0:2:2, respectively. The botany properties and characters of tomato seedling were observed to discuss the application effect of tea-leaf wormcast substrate. The results showed that in all treatments of the compound substrate of tea-leaf wormcast, except of treatment I, the tomato seedling indexes were superior to the control treatments(conventional seedling substrate in market, and the treatments Ⅳ had the best effect, followed by treatment Ⅴ. With the increasing proportion of tea-leaf wormcast, the plant height, stem diameter, SPAD value, and root morphology index of tomato seedlings firstly increased, and then decreased obviously. The substrate with the appropriate proportion of tea-leaf wormcast could obviously improve the quality of tomato seedlings, and the treatment Ⅳ was the best, which could be recommended for the actual production of tomato seedling.

  15. Halophytes As Bioenergy Crops.

    Science.gov (United States)

    Sharma, Rita; Wungrampha, Silas; Singh, Vinay; Pareek, Ashwani; Sharma, Manoj K

    2016-01-01

    Shrinking arable land due to soil salinization and, depleting fresh water resources pose serious worldwide constraints to crop productivity. A vision of using plant feedstock for biofuel production can only be realized if we can identify alternate species that can be grown on saline soils and therefore, would not compete for the resources required for conventional agriculture. Halophytes have remarkable ability to grow under high salinity conditions. They can be irrigated with seawater without compromising their biomass and seed yields making them good alternate candidates as bioenergy crops. Both oil produced from the seeds and the lignocellulosic biomass of halophytes can be utilized for biofuel production. Several researchers across the globe have recognized this potential and assessed several halophytes for their tolerance to salt, seed oil contents and composition of their lignocellulosic biomass. Here, we review current advances and highlight the key species of halophytes analyzed for this purpose. We have critically assessed the challenges and opportunities associated with using halophytes as bioenergy crops.

  16. COMPLEX PROCESSING TECHNOLOGY OF TOMATO RAW MATERIALS

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    A. M. Gadzhieva

    2015-01-01

    Full Text Available Tomatoes grown in the central and southern parts of the country, which contain 5-6 % of solids, including 0.13 % of pectin, 0.86 % of fat, 0.5 % of organic acids; 0.5 % minerals, etc. were used as a subject of research. These tomatoes, grown in the mountains, on soils with high salinity, contain high amounts of valuable components and have a long-term preservation. For the extraction of valuable components from dried tomato pomace CO2 extraction method was applied. Technological and environmental feasibility of tomatoes stage drying in the atmosphere of inert gas in solar dry kiln were evaluated; production scheme of dried tomatoes is improved; a system for tomato pomace drying is developed; a production scheme of powders of pulp, skin and seeds of tomatoes is developed. Combined method of tomato pomace drying involves the simultaneous use of the electromagnetic field of low and ultra-high frequency and blowing product surface with hot nitrogen. Conducting the drying process in an inert gas atmosphere of nitrogen intensified the process of moisture removing from tomatoes. The expediency of using tomato powder as enriching additive was proved. Based on the study of the chemical composition of the tomato powder made from Dagestan varieties of tomatoes, and on the organoleptic evaluation and physico-chemical studies of finished products, we have proved the best degree of recoverability of tomato powder during the production of reconstituted juice and tomato beverages.

  17. Tomato yellow spot virus, a tomato-infecting begomovirus from Brazil with a closer relationship to viruses from Sida sp., forms pseudorecombinants with begomoviruses from tomato but not from Sida.

    Science.gov (United States)

    Andrade, E C; Manhani, G G; Alfenas, P F; Calegario, R F; Fontes, E P B; Zerbini, F M

    2006-12-01

    Geminiviruses are characterized by a circular, single-stranded DNA genome and twinned icosahedral particles. Begomoviruses (whitefly-transmitted geminiviruses) are a major constraint to crop production worldwide. In Brazil, tomato-infecting begomoviruses emerged as serious pathogens over the last 10 years, due to the introduction of a new biotype of the insect vector. Tomato yellow spot virus (ToYSV) is a newly described begomovirus originally isolated from tomato, but phylogenetically closer to viruses from Sida sp. A study was performed to determine the viability of pseudorecombinants formed between the DNA components of ToYSV and other weed- and tomato-infecting begomoviruses from Brazil. Despite its closer relationship to weed-infecting viruses, ToYSV was only capable of forming viable pseudorecombinants with tomato viruses. An infectious pseudorecombinant formed between ToYSV DNA-A and tomato crinkle leaf yellows virus (TCrLYV) DNA-B induced severe symptoms in Nicotiana benthamiana. This was attributed, at least in part, to the fact that the origins of replication of both components had identical Rep-binding sequences. However, this was not the case for another infectious pseudorecombinant formed between tomato golden mosaic virus (TGMV) DNA-A and ToYSV DNA-B, which have different Rep-binding sequences. These results reinforce the notion that pseudorecombinant formation cannot be explained solely on the basis of phylogenetic relationships and conserved iteron sequences, and suggest that the TGMV Rep protein may be more versatile in terms of recognizing heterologous DNA components than that of ToYSV.

  18. Evaluation of Crops Sensitivity to Atrazine Soil Residual

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    E Izadi

    2012-02-01

    Full Text Available Abstract In order to study the sensitivity of 9 crops to atrazine soil residual, two separate experiments were conducted in field and greenhouse conditions. First experiment was conducted in a field with treated soil by atrazine based on split plot and the results evaluated in greenhouse conditions. Treatments in the field experiment included two organic manure application rates (0 and 50 t/ha as main plots and 2 atrazine application rates (2 and 4 kg/ha atrazine a.i. as sob plots. After corn harvesting soil was sampled at 0-15 cm surface layer in each plots in 15 points, after mixing the samples. Wheat, barley, sugar beet, pea, lens and colza planted in pots at greenhouse. Second experiment conducted in greenhouse conditions for evaluation of atrazine soil residual in completely randomized design. Treatments included atrazine soil residual concentrations (0, 0.2, 0.5, 1, 5, 10 and 15 mg/kg soil and crops included wheat, barley, sugar beet, pea, lens, rape, bean and tomato. Results showed that atrazine residue had no effect on crops growth in field experiment treated with atrazine. It seems that atrazine residue in filed soil is lower that its damage threshold for crops or maybe for its fast removal in field than in control conditions. But in bioassay experiment (greenhouse experiment crops response to atrazine residues were different. Results showed that onion and pea were most susceptible ant tolerant crops between studied species and based of ED50 parameter the other crops tolerance to total residue ranked as: pea< bean< lentil< sugar beet< tomato< barley< wheat< rape< onion. Keywords: Atrazine residue, Pea, Bean, Lentil, Sugar beet, Barley, Wheat, Rape, Tomato

  19. Parameterization models for pesticide exposure via crop consumption.

    Science.gov (United States)

    Fantke, Peter; Wieland, Peter; Juraske, Ronnie; Shaddick, Gavin; Itoiz, Eva Sevigné; Friedrich, Rainer; Jolliet, Olivier

    2012-12-04

    An approach for estimating human exposure to pesticides via consumption of six important food crops is presented that can be used to extend multimedia models applied in health risk and life cycle impact assessment. We first assessed the variation of model output (pesticide residues per kg applied) as a function of model input variables (substance, crop, and environmental properties) including their possible correlations using matrix algebra. We identified five key parameters responsible for between 80% and 93% of the variation in pesticide residues, namely time between substance application and crop harvest, degradation half-lives in crops and on crop surfaces, overall residence times in soil, and substance molecular weight. Partition coefficients also play an important role for fruit trees and tomato (Kow), potato (Koc), and lettuce (Kaw, Kow). Focusing on these parameters, we develop crop-specific models by parametrizing a complex fate and exposure assessment framework. The parametric models thereby reflect the framework's physical and chemical mechanisms and predict pesticide residues in harvest using linear combinations of crop, crop surface, and soil compartments. Parametric model results correspond well with results from the complex framework for 1540 substance-crop combinations with total deviations between a factor 4 (potato) and a factor 66 (lettuce). Predicted residues also correspond well with experimental data previously used to evaluate the complex framework. Pesticide mass in harvest can finally be combined with reduction factors accounting for food processing to estimate human exposure from crop consumption. All parametric models can be easily implemented into existing assessment frameworks.

  20. Effects of Red Light Night Break Treatment on Growth and Flowering of Tomato Plants

    OpenAIRE

    Kai eCao; Lirong eCui; Lin eYe; Xiaoting eZhou; Encai eBao; Hailiang eZhao; Zhirong eZou

    2016-01-01

    Compact and healthy young plants increase crop production and improve vegetable quality. Adverse climatic conditions and shading can cause young plants to become elongated and spindly. We investigated the effects of night break (NB) treatments on tomato plants using red light (RL) with an intensity of 20 µmol•m2•s-1. Tomato plants were subjected to NB treatments with different frequencies ranging from every 1, 2, 3, and 4 h, and plant growth, flowering, and yield were monitored. The results s...

  1. Effect of organic amendments and compost extracts on tomato production and storability in ecological production systems

    Directory of Open Access Journals (Sweden)

    ghorbani reza

    2009-06-01

    Full Text Available A field experiment was conducted in Shiravan, Iran, during 2005 in order to investigate the effects of organic amendments, synthetic fertilizers and compost extracts on crop health, productivity and storability of commonly used tomato (Lycopersicon esculentum Mill.. Treatments included different fertilizers of cattle, sheep and poultry manures, house-hold compost and chemical fertilizers, and five aqueous extracts from cattle manure, poultry manures, green-waste and house-hold composts and water as control. The effect of fertilizer type on tomato yield and marketable yield was significant (P

  2. Relay cropping as a sustainable approach: problems and opportunities for sustainable crop production.

    Science.gov (United States)

    Tanveer, Mohsin; Anjum, Shakeel Ahmad; Hussain, Saddam; Cerdà, Artemi; Ashraf, Umair

    2017-03-01

    Climate change, soil degradation, and depletion of natural resources are becoming the most prominent challenges for crop productivity and environmental sustainability in modern agriculture. In the scenario of conventional farming system, limited chances are available to cope with these issues. Relay cropping is a method of multiple cropping where one crop is seeded into standing second crop well before harvesting of second crop. Relay cropping may solve a number of conflicts such as inefficient use of available resources, controversies in sowing time, fertilizer application, and soil degradation. Relay cropping is a complex suite of different resource-efficient technologies, which possesses the capability to improve soil quality, to increase net return, to increase land equivalent ratio, and to control the weeds and pest infestation. The current review emphasized relay cropping as a tool for crop diversification and environmental sustainability with special focus on soil. Briefly, benefits, constraints, and opportunities of relay cropping keeping the goals of higher crop productivity and sustainability have also been discussed in this review. The research and knowledge gap in relay cropping was also highlighted in order to guide the further studies in future.

  3. GENETICALLY MODIFIED FOOD CROPS AND PUBLIC HEALTH

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    Acosta Orlando

    2008-12-01

    Full Text Available The progress made in plant biotechnology has provided an opportunity to new food crops being developed having desirable traits for improving crop yield, reducing the use of agrochemicals and adding nutritional properties to staple crops. However, genetically modified (GM crops have become a subject of intense debate in which opponents argue that GM crops represent a threat to individual freedom, the environment, public health and traditional economies. Despite the advances in food crop agriculture, the current world situation is still characterised by massive hunger and chronic malnutrition, representing a major public health problem. Biofortified GM crops have been considered an important and complementary strategy for delivering naturally-fortified staple foods to malnourished populations. Expert advice and public concern have led to designing strategies for assessing the potential risks involved in cultivating and consuming GM crops. The present critical review was aimed at expressing some conflicting points of view about the potential risks of GM crops for public health. It was concluded that GM food crops are no more risky than those genetically modified by conventional methods and that these GM crops might contribute towards reducing the amount of malnourished people around the world. However, all this needs to be complemented by effective political action aimed at increasing the income of people living below the poverty-line.

  4. Farmers’ knowledge, attitude and practice of healthy greenhouse cucumber and tomato production in Isfahan County

    Directory of Open Access Journals (Sweden)

    A. Sandoghi

    2016-12-01

    Full Text Available In recent decades, due to the negative effects of conventional farming on human health and the environment, the organic farming is taken into consideration in order to produce healthy food materials. The aim of the present study was to assess the greenhouse owners’ knowledge, attitude, and practice with respect to healthy cucumber and tomato production in greenhouse farming systems in Isfahan county, Iran. Required data was collected on a sample of 110 farmers through face-to-face interviews based on a comprehensive structured questionnaire. Behavioral analysis of farmers’ decision making is based on “knowledge, attitude and practice” (KAP survey using structural equation modeling. Research findings showed that despite the positive attitude of most greenhouse owners toward production of healthy crops, there is no proper use of pesticides and fertilizers in greenhouse farming. Although most farmers believe that overuse of pesticides and chemical fertilizers endangers human health and environment, but they don’t adhere to proper usage principles and amount of pesticides and chemical fertilizers. Also, small-scale greenhouses, weak farm economy, low literacy level, and lack of adequate awareness, have led to select the least risky and fastest way to combat the pests. Therefore, it is very important to improve greenhouse owners' knowledge in fertilizer selection and pest management, and strengthening the marketing system of healthy products.

  5. Conventional and phenomics characterization provides insight into the diversity and relationships of hypervariable scarlet (Solanum aethiopicum L. and gboma (S. macrocarpon L. eggplant complexes

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    Mariola ePlazas

    2014-07-01

    Full Text Available Scarlet (Solanum aethiopicum and gboma (S. macrocarpon eggplants are major vegetable crops in sub-Saharan Africa. Together with their respective wild ancestors (S. anguivi and S. dasyphyllum and intermediate cultivated-wild forms they constitute the so-called scarlet and gboma eggplant complexes. We used conventional descriptors and the high-throughput phenomics tool Tomato Analyzer for characterizing 63 accessions of the scarlet eggplant complex, including the four S. aethiopicum cultivar groups (Aculeatum, Gilo, Kumba, and Shum, Intermediate S. aethiopicum-S. anguivi forms, and S. anguivi, and 12 cultivated and wild accessions of the gboma eggplant complex. A large diversity was found between both complexes, showing that they are very well differentiated from each other. Within the scarlet eggplant complex, many significant differences were also found among cultivar groups, but more differences were found for fruit traits evaluated with Tomato Analyzer than with conventional descriptors. In particular, Tomato Analyzer phenomics characterization was useful for distinguishing small fruited groups (Shum, Intermediate, and S. anguivi, as well as groups for which few or no significant differences were observed for plant traits. Multivariate principal components analysis (PCA separated well all groups, except the Intermediate group which plotted between S. anguivi and small fruited S. aethiopicum accessions. For the gboma eggplant complex, S. dasyphyllum was clearly distinguished from S. macrocarpon and an important diversity was found in the latter. The results have shown that both complexes are hypervariable and have provided insight into their diversity and relationships. The information obtained has important implications for the conservation and management of genetic resources as well as for the selection and breeding of both scarlet and gboma eggplants.

  6. Bacterial canker resistance in tomato

    OpenAIRE

    Sen, Y.

    2014-01-01

    Clavibacter michiganensis subsp. michiganensis (Cmm) is the pathogen causing bacterial  canker in tomato. The disease was described for the first time in 1910 in Michigan, USA. Cmmis considered the most harmful bacteria threatening tomato. Disease transmission occurs via seed and symptoms become visible at least 20 days after infection. Due to its complex strategy and transmission, Cmm is under quarantine regulation in EU and other countries. There is no method to stop disease progress i...

  7. PRUNING SYSTEM EFFECT ON GREENHOUSE GRAFTED TOMATO YIELD AND QUALITY

    OpenAIRE

    Mourão, Isabel; Teixeira, Joana; Brito, L Miguel; Ferreira, Maria Elvira; Moura, Maria Luisa

    2014-01-01

    This study aimed to evaluate the effects on tomato yield and quality of three pruning systems (2, 3 and 4 stems) of grafted plants (cv. Vinicio and Multifort) used to prevent the incidence of soil diseases. It was also investigated if the two stems from nodes of the cotyledon leaves improved crop performance compared to the two stems from the first true leaves nodes. The experiment was conducted in the spring/summer season, under greenhouse conditions at NW Portugal, with a randomized block d...

  8. Phytoremediation of soil polluted by nickel using agricultural crops.

    Science.gov (United States)

    Giordani, Cesare; Cecchi, Stefano; Zanchi, Camillo

    2005-11-01

    Soil pollution due to heavy metals is widespread; on the world scale, it involves about 235 million hectares. The objectives of this research were to establish the uptake efficiency of nickel by some agricultural crops. In addition, we wanted to establish also in which part of plants the metal is stored for an eventual use of biomass or for recycling the metal. The experiments included seven herbaceous crops such as: barley (Hordeum vulgaris), cabbage (Brassica juncea), spinach (Spinacea oleracea), sorghum (Sorgum vulgare), bean (Phaseolus vulgaris), tomato (Solanum lycopersicum), and ricinus (Ricinus communis). We used three levels of treatment (150, 300, and 600 ppm) and one control. At the end of the biological cycle of the crops, the different parts of plants, i.e., roots, stems, leaves, fruits, or seeds, were separately collected, oven dried, weighed, milled, and separately analysed. The leaves and stems of spinach showed a very good nickel storage capacity. The ricinus too proved to be a very good nickel storer. The ability of spinach and ricinus to store nickel was observed also in the leaves of cabbage, even if with a lower storage capacity. The bean, barley, and tomato, in decreasing order of uptake and storage capacity, showed a high concentration of nickel in leaves and stems, whereas the sorghum evidenced a lesser capacity to uptake and store nickel in leaves and stems. The bean was the most efficient in storing nickel in fruits or grains. Tomato, sorghum, and barley have shown a storage capacity notably less than bean. The bean appeared to be the most efficient in accumulating nickel in the roots, followed in decreasing order by sorghum, ricinus, and tomato. With regard to the removal of nickel, spinach was the most efficient as it contains the highest level of this metal per gram of dry matter. The ricinus, cabbage, bean, sorghum, barley, and tomato evidenced a progressively decreasing efficiency in the removal of nickel.

  9. Impact of cash cropping and perennial crops on food crop ...

    African Journals Online (AJOL)

    , ... Moreover, more intensive coffee production is associated with more intensive enset ... Keywords: Ethiopia, Cash crops, Food crops, Productivity, Enset. ... household food security at the household level is often based on the income cash.

  10. GENETICALLY MODIFIED CROPS: INTERNATIONAL TRADE AND TRADE POLICY EFFECTS

    Directory of Open Access Journals (Sweden)

    George Frisvold

    2015-04-01

    Full Text Available Where approved, producers have adopted genetically modified (GM crops extensively. Yet, areas not adopting GM crops account for large shares of production and consumption. GM crops differ from previous agricultural innovations because consumers may perceive them as fundamentally different from (and potentially inferior to conventionally grown crops. Many countries maintain restrictions on production and importation of GM crops. GM crop adoption affects producers and consumers, not only through technological change, but also through trade policy responses. This article reviews open economy analyses of impacts of GM crops. To varying degrees, commodities are segmented into GM, conventionally grown, and organic product markets. Recent advances in trade modeling consider the consequences of market segmentation, along with consequences of GM crop import restrictions, product segregation requirements, and coexistence policies.

  11. Tomato (Solanum lycopersicum) variety discrimination and hybridization analysis based on the 5S rRNA region.

    Science.gov (United States)

    Sun, Yan-Lin; Kang, Ho-Min; Kim, Young-Sik; Baek, Jun-Pill; Zheng, Shi-Lin; Xiang, Jin-Jun; Hong, Soon-Kwan

    2014-05-04

    The tomato (Solanum lycopersicum) is a major vegetable crop worldwide. To satisfy popular demand, more than 500 tomato varieties have been bred. However, a clear variety identification has not been found. Thorough understanding of the phylogenetic relationship and hybridization information of tomato varieties is very important for further variety breeding. Thus, in this study, we collected 26 tomato varieties and attempted to distinguish them based on the 5S rRNA region, which is widely used in the determination of phylogenetic relations. Sequence analysis of the 5S rRNA region suggested that a large number of nucleotide variations exist among tomato varieties. These variable nucleotide sites were also informative regarding hybridization. Chromas sequencing of Yellow Mountain View and Seuwiteuking varieties indicated three and one variable nucleotide sites in the non-transcribed spacer (NTS) of the 5S rRNA region showing hybridization, respectively. Based on a phylogenetic tree constructed using the 5S rRNA sequences, we observed that 16 tomato varieties were divided into three groups at 95% similarity. Rubiking and Sseommeoking, Lang Selection Procedure and Seuwiteuking, and Acorn Gold and Yellow Mountain View exhibited very high identity with their partners. This work will aid variety authentication and provides a basis for further tomato variety breeding.

  12. The Solanum lycopersicum WRKY3 Transcription Factor SlWRKY3 Is Involved in Salt Stress Tolerance in Tomato

    Directory of Open Access Journals (Sweden)

    Imène Hichri

    2017-07-01

    Full Text Available Salinity threatens productivity of economically important crops such as tomato (Solanum lycopersicum L.. WRKY transcription factors appear, from a growing body of knowledge, as important regulators of abiotic stresses tolerance. Tomato SlWRKY3 is a nuclear protein binding to the consensus CGTTGACC/T W box. SlWRKY3 is preferentially expressed in aged organs, and is rapidly induced by NaCl, KCl, and drought. In addition, SlWRKY3 responds to salicylic acid, and 35S::SlWRKY3 tomatoes showed under salt treatment reduced contents of salicylic acid. In tomato, overexpression of SlWRKY3 impacted multiple aspects of salinity tolerance. Indeed, salinized (125 mM NaCl, 20 days 35S::SlWRKY3 tomato plants displayed reduced oxidative stress and proline contents compared to WT. Physiological parameters related to plant growth (shoot and root biomass and photosynthesis (stomatal conductance and chlorophyll a content were retained in transgenic plants, together with lower Na+ contents in leaves, and higher accumulation of K+ and Ca2+. Microarray analysis confirmed that many stress-related genes were already up-regulated in transgenic tomatoes under optimal conditions of growth, including genes coding for antioxidant enzymes, ion and water transporters, or plant defense proteins. Together, these results indicate that SlWRKY3 is an important regulator of salinity tolerance in tomato.

  13. Changes in color-related compounds in tomato fruit exocarp and mesocarp during ripening using HPLC-APcI+-mass Spectrometry

    OpenAIRE

    Carrillo-López, A.; Yahia, E.M.

    2012-01-01

    Tomato is an important agricultural crop world-wide. Their pigments are very important in many ways. They have been associated with health benefits such as lowering the risk of some chronic diseases. Quantification of chlorophylls by spectrophotometry and Identification of carotenoids using liquid chromatography coupled to mass spectrometry, and quantification by HPLC-DAD was carried out in the exocarp and mesocarp of tomato fruit during 6 different ripeness stages (mature-green, breakers, tu...

  14. Historical effects of temperature and precipitation on California crop yields

    Energy Technology Data Exchange (ETDEWEB)

    Lobell, D.B. [Energy and Environment Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550 (United States); Cahill, K.N. [Interdisciplinary Graduate Program in Environment and Resources, Stanford University, Stanford, CA 94305 (United States); Field, C.B. [Department of Global Ecology, Carnegie Institution, Stanford, CA 94305 (United States)

    2007-03-15

    For the 1980-2003 period, we analyzed the relationship between crop yield and three climatic variables (minimum temperature, maximum temperature, and precipitation) for 12 major Californian crops: wine grapes, lettuce, almonds, strawberries, table grapes, hay, oranges, cotton, tomatoes, walnuts, avocados, and pistachios. The months and climatic variables of greatest importance to each crop were used to develop regressions relating yield to climatic conditions. For most crops, fairly simple equations using only 2-3 variables explained more than two-thirds of observed yield variance. The types of variables and months identified suggest that relatively poorly understood processes such as crop infection, pollination, and dormancy may be important mechanisms by which climate influences crop yield. Recent climatic trends have had mixed effects on crop yields, with orange and walnut yields aided, avocado yields hurt, and most crops little affected by recent climatic trends. Yield-climate relationships can provide a foundation for forecasting crop production within a year and for projecting the impact of future climate changes.

  15. Weather-based forecasts of California crop yields

    Energy Technology Data Exchange (ETDEWEB)

    Lobell, D B; Cahill, K N; Field, C B

    2005-09-26

    Crop yield forecasts provide useful information to a range of users. Yields for several crops in California are currently forecast based on field surveys and farmer interviews, while for many crops official forecasts do not exist. As broad-scale crop yields are largely dependent on weather, measurements from existing meteorological stations have the potential to provide a reliable, timely, and cost-effective means to anticipate crop yields. We developed weather-based models of state-wide yields for 12 major California crops (wine grapes, lettuce, almonds, strawberries, table grapes, hay, oranges, cotton, tomatoes, walnuts, avocados, and pistachios), and tested their accuracy using cross-validation over the 1980-2003 period. Many crops were forecast with high accuracy, as judged by the percent of yield variation explained by the forecast, the number of yields with correctly predicted direction of yield change, or the number of yields with correctly predicted extreme yields. The most successfully modeled crop was almonds, with 81% of yield variance captured by the forecast. Predictions for most crops relied on weather measurements well before harvest time, allowing for lead times that were longer than existing procedures in many cases.

  16. Experimental delayed-light-emission meter for horticultural crops

    Science.gov (United States)

    Forbus, W. R.; Hardigree, G. A.; Adams, J. H.

    1985-09-01

    Equipment for measuring the delayed light emission (DLE) from horticultural crops was developed for use in a long-range study to determine the feasibility of sorting different fruits and vegetables according to maturity by measuring the light emitted from them several seconds after illumination by a controlled light source. Preliminary tests with tomatoes showed that the magnitude of detector response to DLE decreased during apparent ripening as indicated by an increase in red color.

  17. TOMATOMICS: A Web Database for Integrated Omics Information in Tomato

    KAUST Repository

    Kudo, Toru

    2016-11-29

    Solanum lycopersicum (tomato) is an important agronomic crop and a major model fruit-producing plant. To facilitate basic and applied research, comprehensive experimental resources and omics information on tomato are available following their development. Mutant lines and cDNA clones from a dwarf cultivar, Micro-Tom, are two of these genetic resources. Large-scale sequencing data for ESTs and full-length cDNAs from Micro-Tom continue to be gathered. In conjunction with information on the reference genome sequence of another cultivar, Heinz 1706, the Micro-Tom experimental resources have facilitated comprehensive functional analyses. To enhance the efficiency of acquiring omics information for tomato biology, we have integrated the information on the Micro-Tom experimental resources and the Heinz 1706 genome sequence. We have also inferred gene structure by comparison of sequences between the genome of Heinz 1706 and the transcriptome, which are comprised of Micro-Tom full-length cDNAs and Heinz 1706 RNA-seq data stored in the KaFTom and Sequence Read Archive databases. In order to provide large-scale omics information with streamlined connectivity we have developed and maintain a web database TOMATOMICS (http://bioinf.mind.meiji.ac.jp/tomatomics/). In TOMATOMICS, access to the information on the cDNA clone resources, full-length mRNA sequences, gene structures, expression profiles and functional annotations of genes is available through search functions and the genome browser, which has an intuitive graphical interface.

  18. Economic evaluation of cereal cropping systems under semiarid conditions: minimum input, organic and conventional Avaliação econômica de sistemas de cultivo de cereias em condições semiáridas: cultivo mínimo, orgânico e convencional

    Directory of Open Access Journals (Sweden)

    Gabriel Pardo

    2009-10-01

    Full Text Available Cropping systems like organic farming, selling products at a higher price and promoting environmental sustainability by reducing fertilizer and pesticides, can be more profitable than conventional systems. An economic evaluation of three cropping systems in a seven year period experiment was performed, using a common rotation (fallow-barley-vetch-durum wheat in a semi-arid rainfed field of Spain. The minimum input system included mouldboard ploughing, cultivator preparation, sowing and harvest. The conventional system involved mineral fertilizer and herbicide treatments, while the organic system involved composted manure and mechanical weed control. The resulting economic margins were highest with the minimum input system, followed by the organic and conventional systems. If the cereal grain from the minimum input system was sold at a higher price on the organic market, this system was the most profitable. Without the price difference, the organic system was as profitable as the conventional one.Sistemas de cultivo como agricultura orgânica, cujos produtos têm custo mais alto, mas que promova sustentabilidade ambiental pela redução do uso de fertilizantes e pesticidas, pode ser mais lucrativo do que sistemas convencionais de cultivo. Efetuou-se uma avaliação econômica de três sistemas de cultivo num período experimental de sete anos, utilizando um sistema comum de rotação (pousio-cevada-ervilhaca-trigo duro, sem irrigação, em um local semi-árido da Espanha. O sistema de cultivo mínimo incluía aração com aiveca, preparo com cultivador, semeadura e colheita. O sistema convencional envolvia tratamentos com herbicidas e fertilizantes minerais, enquanto o sistema orgânico envolvia adubação com composto e controle mecânico de ervas invasoras. O sistema com maior retorno econômico foi o de cultivo mínimo, seguido do orgânico e do convencional. O sistema de cultivo mínimo foi o mais lucrativo quando o cereal foi vendido num pre

  19. Biological Constraints in Tomato Production in the Western Highlands of Cameroon

    Directory of Open Access Journals (Sweden)

    Fontem, DA.

    1999-01-01

    Full Text Available Tomato (Lycopersicon esculentum production is handicapped by damage due to pests and pathogens. Farmers' fields in the western highlands of Cameroon were surveyed during 1993 to 1996 to identify biological constraints in production. Diseases and insect pests are the most important biological limitations in tomato production. Late blight caused by Phytophthora infestans and early blight caused by Alternaria solani are the most severe diseases, while the melon fruitfly (Dacus cucurbitae is the most prevalent insect pest. Yield losses due to pest damage are high and reach 100 % when the crop is not treated in the wet season. Pest-resistant varieties are not available to farmers. Consequently, growers practise intensive pesticidal spray programmes to limit losses caused by pests and diseases. Results indicate the necessity for the adoption of integrated pest management strategies in tomato production in Cameroon.

  20. Domestication selected for deceleration of the circadian clock in cultivated tomato.

    Science.gov (United States)

    Müller, Niels A; Wijnen, Cris L; Srinivasan, Arunkumar; Ryngajllo, Malgorzata; Ofner, Itai; Lin, Tao; Ranjan, Aashish; West, Donnelly; Maloof, Julin N; Sinha, Neelima R; Huang, Sanwen; Zamir, Dani; Jiménez-Gómez, José M

    2016-01-01

    The circadian clock is a critical regulator of plant physiology and development, controlling key agricultural traits in crop plants. In addition, natural variation in circadian rhythms is important for local adaptation. However, quantitative modulation of circadian rhythms due to artificial selection has not yet been reported. Here we show that the circadian clock of cultivated tomato (Solanum lycopersicum) has slowed during domestication. Allelic variation of the tomato homolog of the Arabidopsis gene EID1 is responsible for a phase delay. Notably, the genomic region harboring EID1 shows signatures of a selective sweep. We find that the EID1 allele in cultivated tomatoes enhances plant performance specifically under long day photoperiods, suggesting that humans selected slower circadian rhythms to adapt the cultivated species to the long summer days it encountered as it was moved away from the equator.

  1. Deficit irrigation based on drought tolerance and root signalling in potatoes and tomatoes

    DEFF Research Database (Denmark)

    Jensen, Christian Richardt; Battilani, Adriano; Plauborg, Finn

    2010-01-01

    esculentum Mill.) and processing tomatoes as model plants. From the pot and semi-field experiments an ABA production model was developed for potatoes to optimize the ABA signalling; this was obtained by modelling the optimal level of soil drying for ABA production before re-irrigation in a crop growth model......Agriculture is a big consumer of fresh water in competition with other sectors of the society. Within the EU-project SAFIR new water-saving irrigation strategies were developed based on pot, semi-field and field experiments with potatoes (Solanum tuberosum L.), fresh tomatoes (Lycopersicon....... The field irrigation guidelines were developed under temperate (Denmark), Mediterranean (Greece, Italy) and continental (Serbia, China) climatic conditions during summer. The field investigations on processing tomatoes were undertaken only in the Po valley (North Italy) on fine, textured soil...

  2. Fungal endophytes – the hidden inducers of volatile terpene biosynthesis in tomato plants

    DEFF Research Database (Denmark)

    Ntana, Fani; Jensen, Birgit; Jørgensen, Hans Jørgen Lyngs

    and herbivores. Tomato (Solanum lycopersicum) is an important crop, often challenged by fungal pathogens and insect pests. The wide variety of secondary metabolites produced by the plant, and especially terpenes, play a crucial role in plant defence, helping in repelling possible enemies. This project is focused...... on establishing a balanced interaction between S. indica and tomato in vitro, as well as reliable detection methods that show fungal colonization of inoculated plant roots. The effect of root colonization by S. indica on host specialized metabolism is also determined, by comparing volatile terpene profiles of S....... indica-inoculated and S. indica-free tomato plants. Preliminary data suggest that fungal colonization results in increased production of specific volatile terpenes. A transcriptome analysis on fungus-associated and fungus-free plant tissues is currently ongoing to elucidate in depth the mechanisms...

  3. Effects of phosphorus fertilizer supplementation on processing quality and functional food ingredients in tomato.

    Science.gov (United States)

    Oke, Moustapha; Ahn, Taehyun; Schofield, Andrew; Paliyath, Gopinadhan

    2005-03-09

    Even though several types of phosphorus fertilizers are used in crop production, the influence of phosphorus on produce quality is not well understood. Several quality attributes of tomato juice were analyzed in relation to phosphorus supplementation during a three-year field study (2000-2002). In addition to the recommended phosphorus fertilization, phosphorus supplementations, either through soil (low and high) or through foliar spray (hydrophos, seniphos), were tested. In general, soil and foliar phosphorus supplementation did not provide a statistically significant increase in yield. Tomato juice was evaluated for various quality characteristics including pH, titratable acidity, precipitate weight ratio, total solids, serum viscosity, Brookfield viscosity, color, lycopene levels, vitamin C, and flavor volatiles. Changes observed in several quality parameters were marginal, statistically insignificant and influenced by the season. Therefore, it appears that phosphorus supplementation may not significantly affect the processing quality parameters in tomato fruits.

  4. Protocol: fine-tuning of a Chromatin Immunoprecipitation (ChIP protocol in tomato

    Directory of Open Access Journals (Sweden)

    Iusem Norberto D

    2010-04-01

    Full Text Available Abstract Background Searching thoroughly for plant cis-elements corresponding to transcription factors is worthwhile to reveal novel gene activation cascades. At the same time, a great deal of research is currently focused on epigenetic events in plants. A widely used method serving both purposes is chromatin immunoprecipitation, which was developed for Arabidopsis and other plants but is not yet operational for tomato (Solanum lycopersicum, a model plant species for a group of economically important crops. Results We developed a chromatin immunoprecipitation protocol suitable for tomato by adjusting the parameters to optimise in vivo crosslinking, purification of nuclei, chromatin extraction, DNA shearing and precipitate analysis using real-time PCR. Results were obtained with two different antibodies, five control loci and two normalisation criteria. Conclusion Here we provide a chromatin immunoprecipitation procedure for tomato leaves that could be combined with high-throughput sequencing to generate a detailed map of epigenetic modifications or genome-wide nucleosome positioning data.

  5. Detection and molecular characterization of tomato yellow leaf curl virus naturally infecting Lycopersicon esculentum in Egypt.

    Science.gov (United States)

    Rabie, M; Ratti, C; Abdel Aleem, E; Fattouh, F

    2017-01-01

    Tomato yellow leaf curl virus (TYLCV) infections of tomato crops in Egypt were widely spread in 2014. Infected symptomatic tomato plants from different governorates were sampled. TYLCV strains Israel and Mild (TYLCV-IL, TYLCV-Mild) were identified by multiplex and real-time PCR. In addition, nucleotide sequence analysis of the V1 and V2 protein genes, revealed ten TYLCV Egyptian isolates (TYLCV from TY1 to 10). Phylogenetic analysis showed their high degree of relatedness with TYLCV-IL Jordan isolate (98%). Here we have showed the complete nucleotide sequence of the TYLCV Egyptian isolate TY10, sampled from El Beheira. A high degree of similarity to other previously reported Egyptian isolates and isolates from Jordan and Japan reflect the importance of phylogenetic analysis in monitoring virus genetic diversity and possibilities for divergence of more virulent strains or genotypes.

  6. RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits

    NARCIS (Netherlands)

    Schijlen, E.G.W.M.; Vos, de C.H.; Martens, S.; Jonker, H.H.; Rosin, F.M.A.; Molthoff, J.W.; Tikunov, Y.M.; Angenent, G.C.; Tunen, van A.J.; Bovy, A.G.

    2007-01-01

    Parthenocarpy, the formation of seedless fruits in the absence of functional fertilization, is a desirable trait for several important crop plants, including tomato (Solanum lycopersicum). Seedless fruits can be of great value for consumers, the processing industry, and breeding companies. In this a

  7. Cross-protection or enhanced symptom display in greenhouse tomato co-infected with different Pepino mosaic virus isolates

    NARCIS (Netherlands)

    Hanssen, I.M.; Gutiérrez-Aguirre, I.; Paeleman, A.; Goen, K.; Wittemans, L.; Lievens, B.; Vanachter, A.C.R.C.; Ravnikar, M.; Thomma, B.P.H.J.

    2010-01-01

    The potential of three mild Pepino mosaic virus (PepMV) isolates, belonging to the CH2, EU and LP genotypes, to protect a tomato (Solanum lycopersicum) crop against an aggressive challenge isolate of the CH2 genotype was assessed in greenhouse trials and PepMV symptoms were rated at regular time poi

  8. Biological control of Trialeurodes vaporariorum by Encarsia formosa on tomato in unheated greenhouses in the high altitude tropics

    NARCIS (Netherlands)

    Vis, de R.M.J.; Lenteren, van J.C.

    2008-01-01

    Biological control of Trialeurodes vaporariorum (Westwood) by Encarsia formosa Gahan was tested during three consecutive production cycles (16-28 weeks) on a beef tomato (Solanum lycopersicum L.) crop in a glasshouse and a plastic greenhouse on the Bogota Plateau in Colombia. During the course of th

  9. Reproductive performance of seven strains of the tomato red spider mite Tetranychus evansi (Acari: Tetranychidae) at five temperatures

    DEFF Research Database (Denmark)

    Gotoh, T.; Sugimoto, N.; Pallini, A.;

    2010-01-01

    The tomato red spider mite Tetranychus evansi Baker et Pritchard occurs on solanaceous plants, and causes serious damage to a variety of crops in Africa and Europe. In 2001 this species was also found in Japan, on nightshade (Solanum nigrum L.), and its invasion to solanaceous of agricultural...

  10. RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits

    NARCIS (Netherlands)

    Schijlen, E.G.W.M.; Vos, de C.H.; Martens, S.; Jonker, H.H.; Rosin, F.M.A.; Molthoff, J.W.; Tikunov, Y.M.; Angenent, G.C.; Tunen, van A.J.; Bovy, A.G.

    2007-01-01

    Parthenocarpy, the formation of seedless fruits in the absence of functional fertilization, is a desirable trait for several important crop plants, including tomato (Solanum lycopersicum). Seedless fruits can be of great value for consumers, the processing industry, and breeding companies. In this

  11. Cross-protection or enhanced symptom display in greenhouse tomato co-infected with different Pepino mosaic virus isolates

    NARCIS (Netherlands)

    Hanssen, I.M.; Gutiérrez-Aguirre, I.; Paeleman, A.; Goen, K.; Wittemans, L.; Lievens, B.; Vanachter, A.C.R.C.; Ravnikar, M.; Thomma, B.P.H.J.

    2010-01-01

    The potential of three mild Pepino mosaic virus (PepMV) isolates, belonging to the CH2, EU and LP genotypes, to protect a tomato (Solanum lycopersicum) crop against an aggressive challenge isolate of the CH2 genotype was assessed in greenhouse trials and PepMV symptoms were rated at regular time

  12. The Cf-4 and Cf-9 resistance proteins of tomato: molecular aspects of specificity and elicitor perception

    NARCIS (Netherlands)

    Hoorn, van der R.A.L.

    2001-01-01

    To feed the increasing world population, agricultural production needs continuous improvement. Especially protection of crops from disastrous diseases is crucial. The interaction between the pathogenic fungus Cladosporium fulvum and its host, tomato, serves as a model system for p

  13. Genetic dissection of novel QTLs for resistance to leaf spots and Tomato spotted wilt virus in peanut (Arachis hypogaea L.)

    Science.gov (United States)

    Peanut is an import crop, economically and nutritiously, but high production cost is a serious challenge to peanut farmers as exemplified by chemical spray to control foliar diseases such as leaf spots and thrips, the vectors of tomato spotted wilt virus (TSWV). The objective of this research was to...

  14. Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.

    Science.gov (United States)

    Arshad, Waheed; Haq, Ihsan-ul-; Waheed, Mohammad Tahir; Mysore, Kirankumar S; Mirza, Bushra

    2014-01-01

    Tomato (Solanum lycopersicum L.) is the second most important cultivated crop next to potato, worldwide. Tomato serves as an important source of antioxidants in human diet. Alternaria solani and Fusarium oxysporum cause early blight and vascular wilt of tomato, respectively, resulting in severe crop losses. The foremost objective of the present study was to generate transgenic tomato plants with rolB gene and evaluate its effect on plant morphology, nutritional contents, yield and resistance against fungal infection. Tomato cv. Rio Grande was transformed via Agrobacterium tumefaciens harbouring rolB gene of Agrobacterium rhizogenes. rolB. Biochemical analyses showed considerable improvement in nutritional quality of transgenic tomato fruits as indicated by 62% increase in lycopene content, 225% in ascorbic acid content, 58% in total phenolics and 26% in free radical scavenging activity. Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum. Contrarily, transformed plants exhibited altered morphology and reduced fruit yield. In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.

  15. Propriedades físicas do solo sob preparo convencional e semeadura direta em rotação e sucessão de culturas, comparadas às do campo nativo Physical soil properties of conventional tillage and no-tillage, in crop rotation and succession, compared with natural pasture

    Directory of Open Access Journals (Sweden)

    I. Bertol

    2004-02-01

    sustentabilidade da semeadura direta no que se refere à estabilidade dos agregados em água. Os sistemas de cultivo não influenciaram as propriedades físicas do solo.In soils with naturally favorable characteristics for cultivation, conventional tillage degrades the physical soil properties, since this management system causes the rupture of aggregates, soil compaction, and eliminates soil cover. No-tillage, on the other hand, maintains soil cover and improves physical properties, but consolidates the surface layer. Our study was conducted on a Haplumbrept soil, from May 1995 to April 2001. Five soil tillage treatments were used: conventional tillage crop rotation (CTR, conventional tillage crop succession (CTS, no-tillage crop rotation (NTR, no-tillage crop succession (NTS, and natural pasture (NP, in four replications each. The crop sequences were bean/fallow/maize/fallow/soybean/fallow in CTR, maize/fallow/maize/fallow/maize/fallow in CTS, bean/oat/maize/turnip/soybean/vetch in NTR and maize/vetch/maize/vetch/maize/vetch in NTS. Soil density, macroporosity, microporosity, total porosity, organic carbon, and water aggregate stability (MWD were evaluated in April 2001 for the soil layers 0-2.5, 2.5-5, 5-10, 10-15, 15-20, and 20-30 cm. In the 0-10 cm layer, soil density was higher in no-tillage than conventional tillage and natural pasture, while macroporosity, total porosity, and the macroporosity/total porosity relation was higher in conventional tillage in the mean for cropping systems, in this layer. Organic carbon, MWD, and sensibility index for MWD means of layers and tillage systems were higher in no-tillage and natural pasture than in conventional tillage.

  16. Carotenes in processed tomato after thermal treatment

    NARCIS (Netherlands)

    Luterotti, S.; Bicanic, D.D.; Markovic, K.; Franko, M.

    2015-01-01

    This report adds to the ongoing vivid dispute on the fate of carotenes in tomato upon thermal processing. Although many papers dealing with changes in the raw tomatoes during industrial treatment have already appeared, data on the fate of finished, processed tomato products when they are additionall

  17. 21 CFR 156.145 - Tomato juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Tomato juice. 156.145 Section 156.145 Food and... CONSUMPTION VEGETABLE JUICES Requirements for Specific Standardized Vegetable Juices § 156.145 Tomato juice. (a) Identity—(1) Definition. Tomato juice is the food intended for direct consumption, obtained...

  18. Avaliação do desempenho de um sistema automático para controle da fertirrigação do tomateiro cultivado em substrato Performance evaluation of an automatic system for tomato fertigation control in substrate

    Directory of Open Access Journals (Sweden)

    Antonio J. Steidle Neto

    2009-09-01

    Full Text Available Este trabalho teve por objetivo avaliar o desempenho de um sistema de controle automático de fertirrigação para a produção do tomateiro em substrato de areia, comparativamente ao sistema de controle convencional quanto à redução de solução nutritiva. No método de controle automático, os eventos de fertirrigação foram estabelecidos em função das condições meteorológicas do ambiente de cultivo e do estádio de desenvolvimento da cultura. Para isso, o modelo de Penman-Monteith foi utilizado como suporte para a tomada de decisão sobre a frequência adequada para aplicação da solução nutritiva. No sistema de controle convencional, os intervalos entre as fertirrigações permaneceram fixos durante todo o ciclo do tomateiro. Os resultados demonstraram que o sistema de controle automático atendeu plenamente às necessidades hídricas da cultura, sem comprometer a produção do tomateiro, proporcionando reduções expressivas no consumo de solução nutritiva. Por outro lado, o sistema de controle convencional realizou número excessivo de fertirrigações, principalmente durante o estádio inicial de desenvolvimento do tomateiro e nos dias caracterizados por elevada nebulosidade. No estádio inicial de crescimento, verificou-se que os volumes totais de solução nutritiva, aplicados ao tomateiro pelo sistema convencional, excederam as necessidades hídricas da cultura em 1,31 e 1,39 L planta-1 em dias típicos com céu claro e nublado, respectivamente.The objective of this work was to compare the performance of an automatic fertigation control system, for soilless tomato production in sand substrate, as compared to a conventional control system. In the automatic control, fertigation events were established by meteorological conditions in the cultivation environment and crop development stage. In this way, the Penman-Monteith model was utilized as a decision support tool regarding the appropriate frequency for delivering the

  19. Ectopic expression of Arabidopsis RCI2A gene contributes to cold tolerance in tomato.

    Science.gov (United States)

    Sivankalyani, Velu; Geetha, Mahalingam; Subramanyam, Kondeti; Girija, Shanmugam

    2015-04-01

    Cold is a major stress that limits the quality and productivity of economically important crops such as tomato (Solanum lycopersicum L.). Generating a cold-stress-tolerant tomato by expressing cold-inducible genes would increase agricultural strategies. Rare cold-inducible 2a (RCI2A) is expressed in Arabidopsis, but its molecular function during cold stress is not fully understood. Here we ectopically expressed Arabidopsis RCI2A in transgenic tomato to evaluate tolerance to cold stress without altering agronomic traits. Biochemical and physiological study demonstrated that expression of RCI2A in transgenic tomato enhanced the activity of peroxidase and ascorbate peroxidase (APX) and reduced the accumulation of H2O2, alleviated lipid peroxidation, increased the accumulation of chlorophyll, reduced chilling-induced membrane damage, retained relative water content and enhanced cold tolerance. A motif search revealed that the motifs of photosystem II (PSII) phosphoproteins PsbJ and PsbH and reaction-center proteins PsbL and PsbK were common to cold-inducible RCI2A and peroxidase proteins RCI3A, tomato peroxidase (TPX1), TPX2, tomato ascorbate peroxidase (APX1), and horseradish peroxidase (HRP-c). In addition to membrane protection, RCI2A may cross talk with PSII-associated proteins or peroxidase family enzymes in response to cold stress. Our findings may strengthen the understanding of the molecular function of RCI2A in cold-stress tolerance. RCI2A could be used to improve abiotic stress tolerance in agronomic crops.

  20. Abscisic acid root and leaf concentration in relation to biomass partitioning in salinized tomato plants.

    Science.gov (United States)

    Lovelli, Stella; Scopa, Antonio; Perniola, Michele; Di Tommaso, Teodoro; Sofo, Adriano

    2012-02-15

    Salinization is one of the most important causes of crop productivity reduction in many areas of the world. Mechanisms that control leaf growth and shoot development under the osmotic phase of salinity are still obscure, and opinions differ regarding the Abscisic acid (ABA) role in regulation of biomass allocation under salt stress. ABA concentration in roots and leaves was analyzed in a genotype of processing tomato under two increasing levels of salinity stress for five weeks: 100 mM NaCl (S10) and 150 mM NaCl (S15), to study the effect of ABA changes on leaf gas exchange and dry matter partitioning of this crop under salinity conditions. In S15, salinization decreased dry matter by 78% and induced significant increases of Na(+) and Cl(-) in both leaves and roots. Dry matter allocated in different parts of plant was significantly different in salt-stressed treatments, as salinization increased root/shoot ratio 2-fold in S15 and 3-fold in S15 compared to the control. Total leaf water potential (Ψ(w)) decreased from an average value of approximately -1.0 MPa, measured on control plants and S10, to -1.17 MPa in S15. In S15, photosynthesis was reduced by 23% and stomatal conductance decreased by 61%. Moreover, salinity induced ABA accumulation both in tomato leaves and roots of the more stressed treatment (S15), where ABA level was higher in roots than in leaves (550 and 312 ng g(-1) fresh weight, respectively). Our results suggest that the dynamics of ABA and ion accumulation in tomato leaves significantly affected both growth and gas exchange-related parameters in tomato. In particular, ABA appeared to be involved in the tomato salinity response and could play an important role in dry matter partitioning between roots and shoots of tomato plants subjected to salt stress.

  1. Condições de trabalho associadas ao uso de agrotóxicos na cultura de tomate de mesa em Goiás Work conditions associated to the use of pesticides in the tomato crop in Goiás

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    Sueli Martins Freitas Alves

    2008-12-01

    Full Text Available O Estado de Goiás, em 2005 foi o maior produtor de tomate do Brasil. Para garantir a produção e controlar as pragas que atacam essa cultura, o tomaticultor apóia-se em pacotes tecnológicos utilizando a aplicação sistemática de produtos químicos. Objetivou-se, neste trabalho, conhecer o cenário no qual os trabalhadores da cultura de tomate em Goiás estão inseridos. Foram selecionados, por meio de amostragem sistemática, seis municípios: Bonfinópolis, Corumbá de Goiás, Goianápolis, Leopoldo de Bulhões, Pirenópolis e Silvânia. Os dados foram obtidos a partir das visitas de campo realizadas no período de dezembro de 2004 a outubro de 2005 e foram utilizados os seguintes instrumentos: entrevista, questionário proposto aos trabalhadores que manipulam os agrotóxicos e a técnica da observação livre. A partir da análise das informações foi possível concluir que esses trabalhadores estão constantemente expostos aos agrotóxicos e há uma falta de preparo para a manipulação dessas substâncias.In 2005, Goiás state was the greater tomato producer in Brazil. To improve the harvest and to keep the tomato disease under control, the tomato producer makes use of a technological packing kit with systematic application of many chemical substances. The major purpose of this thesis is to describe the daily conditions that the tomato plantation workers are subordinated in Goiás State. The research was obtained through systematic sampling directly in the tomato plantation in a period range from December 2004 to October 2005 in six selected cities: Bonfinópolis, Corumbá de Goiás, Goianápolis, Leopoldo de Bulhoes, Perenópolis, and Silvânia. The data were collected from interview, questionnaire to be answered by the workers that manipulate pesticides, and making register of the facts observed during visitation in the tillage. From the data analysis it is possible to conclude that the tomato tillage workers are exposed for long

  2. Tomato plant inheritance of antixenotic resistance to tomato leafminer

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    Adilson de Castro Antônio

    2011-01-01

    Full Text Available The objective of this work was to determine the inheritance of resistance by antixenosis in tomato plants (Lycopersicon esculentum to tomato leafminer [Tuta absoluta (Lepidoptera: Gelechiidae]. Evaluations were performed for tomato plants of the generations P1, P2, F1, F2, RC1 and RC2. The measured characteristic in the parents, BGH-1497 (P2 male and 'Santa Clara' (P1 female, and in the F1, F2, RC1 and RC2 generations was the number of eggs per plant. This number was converted to the oviposition nonpreference index. The inheritance of antixenosis resistance of genotype BGH-1497 is ruled by a gene of greater effect and polygenes in epistatic interactions, with a phenotypic proportion of 13:3 between susceptible and resistant genotypes, respectively.

  3. Simulating Stochastic Crop Management in Cropping Systems

    Science.gov (United States)

    Introduction -- Crop simulation models are uniquely suitable for examining long term crop responses to environmental variability due to changes in climate or other factors. Long-term studies typically emphasize variability related to weather conditions; certain weather-dependent cropping practices m...

  4. Altura de planta e acúmulo de matéria seca do feijoeiro cvs. BRS MG Talismã e Ouro Negro em plantio direto e convencional Plant height and dry matter acumulation by common bean cvs. BRS MG Talismã and Ouro Negro under no-tillage and conventional crop systems

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    Neiva Maria Batista Vieira

    2008-12-01

    Full Text Available Com o objetivo de estudar a curva de crescimento em altura de planta, taxa e acúmulo de matéria seca da parte aérea ao longo do ciclo cultural do feijoeiro (Phaseolus vulgaris L. foram conduzidos quatro experimentos de campo, com as cvs. Ouro Negro e BRS MG Talismã, em plantio direto e convencional, sendo um em Lavras (Latossolo Vermelho distroférrico típico, inverno primavera 2002 e os demais em Madre de Deus de Minas (Latossolo Vermelho Amarelo ácrico, seca 2005. O delineamento experimental foi feito com blocos casualizados com três repetições e 11 ou 12 tratamentos (épocas de coleta. De 7 em 7 dias foram amostradas 10 ou 20 plantas para determinação da altura, através do comprimento da haste principal, e outras 20 plantas para determinação do peso da matéria seca da parte aérea após secagem em estufa com circulação de ar a 65-70ºC até peso constante. Os dados foram submetidos a análises de variância e regressão, com ajuste de curvas representativas do comportamento das características avaliadas. Em plantio direto, a altura máxima foi alcançada aos 50 DAE, enquanto no plantio convencional o feijoeiro continuou crescendo até os 72-73 DAE, ocorrendo fechamento mais tardio da lavoura. As cvs. Ouro Negro e BRS MG Talismã mostraram padrões de acúmulo de matéria seca muito próximos, com pouco incremento até os 81 DAE, na BRS MG Talismã, e ambas alcançaram maiores acúmulos em plantio direto. Em geral, as maiores taxas de acúmulo de matéria seca foram verificadas entre 45-48 DAE.The aim of this research was to study the plant growth in height and the dry matter accumulation pattern along the bean (Phaseolus vulgaris L. crop cycle. Four field experiments were carried out in Lavras (winter-spring 2002 and other were performed in Madre de Deus de Minas, MG (dry crop season 2005 with common bean, cvs. Ouro Negro and BRS MG Talismã, under no-tillage and conventional crop systems. Every seven days 10 or 20 plants

  5. Atributos químicos de um Cambissolo Húmico após 12 anos sob preparo convencional e semeadura direta em rotação e sucessão de culturas Chemical properties of a Humic Dystrudept after 12 years under conventional and no tillage with crop succession and rotation

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    Andréia Patrícia Andrade

    2012-05-01

    Full Text Available O manejo adequado do solo é uma das bases para a sustentabilidade do sistema agrícola. O objetivo do estudo foi avaliar atributos químicos de um Cambissolo Húmico Alumínico após 12 anos em dois sistemas de preparo do solo, com rotação e sucessão de culturas. O experimento foi conduzido em Lages, SC, utilizando preparo convencional (PC e semeadura direta (SD, sob rotação (r e sucessão (s de culturas. O delineamento experimental foi inteiramente casualizado com quatro repetições. As sequências de cultivo foram: feijão-pousio-milho-pousio-soja-pousio no PCr; milho-pousio no PCs; feijão-aveia-milho-nabo-soja-ervilhaca na SDr; e milho-ervilhaca na SDs. As amostras de solo foram coletadas nas camadas 0-2,5; 2,5-5; 5-10; e 10-20cm. Avaliou-se carbono orgânico total (COT, cálcio, magnésio, alumínio trocável, fósforo, potássio, nitrogênio total (NT e pH em água. A SD aumentou os teores de COT e nutrientes em comparação ao PC, especialmente na camada superficial do solo. O cultivo de milho e ervilhaca em sucessão aumentou os teores de COT e NT na camada superficial do solo em comparação com a rotação sob semeadura direta.Suitable soil management is one of the bases for sustainability in agricultural systems. The study aimed to evaluate chemical properties of a Humic Dystrudept for 12 years under two tillage systems, with crops rotation and succession. The experiment was carried out in Lages, SC, under conventional tillage (CT and no-till (NT, with rotation (r and succession (s cropping systems, using crop sequences of beans-fallow-maize-fallow-soybean in CTr; maize-fallow in CTs; beans-oats-maize-fodder radish-soybean-vetch in NTr; and maize-vetch in NTs. The experimental design was completely randomized with four replicates. The soil samples were collected in the layers 0-2.5, 2.5-5, 5-10 and 10-20cm. The variables assessed were total organic carbon (TOC, calcium, magnesium, exchangeable aluminum, phosphorus, potassium

  6. Potential adaptation of a Q biotype whitefly population from poinsettia to field crops

    Institute of Scientific and Technical Information of China (English)

    Xiangshun Hu; Timothy J. Dennehy; Xinzhi Ni; Huiyan Zhao; Robert L. Nichols; Xianchun Li

    2011-01-01

    The invasive Q biotype whitefly was first detected in the US on poinsettia in 2004 and is still not a pest outside of greenhouse environments in the US.To assess the potential for the establishment of the Q biotype on field crops,population cage experiments were conducted to compare the performance of a poinsettia-derived Q population named P'06 on poinsettia and six field crops (alfalfa,tomato,melon,cotton,cowpea and cabbage).P'06 adults reared on poinsettia as nymphs laid eggs on all six field crops.Significantly more eggs were laid on alfalfa,tomato,melon and cotton than on cabbage,cowpea and poinsettia.These eggs hatched and the nymphs developed to adults on the six field crops.Relative to poinsettia,whitefly survival was similar on cowpea,alfalfa,tomato and cabbage,but significantly higher on cotton and melon.Moreover,P'06 had significantly shorter development times from egg to adult on cotton,melon,cowpea,tomato and alfalfa than they did on poinsettia.However,the F 1 adults raised on the six field crops had significantly shorter lifespans and laid 11- to 18-fold fewer eggs than did the F1 adults raised on poinsettia.Taken together,while P'06 may have some potential to establish on field crops,the shorter lifespans and extremely low fecundities of the F1 adults raised on the six field crops suggests that P'06 is incapable of rapidly adapting to them.Poor adaptation to field crops may explain,at least partially,why the Q biotype has not established in the US field system.

  7. Alterações microbianas no solo durante o ciclo do milho nos sistemas plantio direto e convencional Microbial changes in soil during a maize crop season in no-till and conventional systems

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    Luciano Kayser Vargas

    2004-08-01

    Full Text Available A disponibilidade de resíduos de aveia-preta, com relação C:N elevada, resulta em imobilização microbiana de nitrogênio no solo, exigindo cuidados no manejo da adubação nitrogenada da cultura subseqüente. O objetivo deste trabalho foi avaliar as alterações na estrutura da comunidade microbiana ao longo do ciclo do milho, na presença de resíduos de aveia-preta e da aplicação de nitrogênio. Foram coletadas amostras de um Argissolo Vermelho distrófico no dia da semeadura do milho e 46, 62, 88 e 112 dias após a semeadura. O nitrogênio foi aplicado 25 dias e 49 dias após a semeadura. As alterações na comunidade microbiana foram avaliadas mediante relações entre carbono (C e nitrogênio (N, nitrogênio reativo com ninidrina (N-Nin e carboidratos (CHO da biomassa microbiana, além da análise do rDNA fúngico e bacteriano. As diferenças na composição da comunidade microbiana, reveladas pela análise do rDNA, relacionaram-se mais com as relações C:N e C:N-Nin do que com a relação C:CHO. As relações C:N-Nin e C:N e as avaliações do rDNA mostraram um predomínio inicial de população fúngica. Com a aplicação de N, a população bacteriana tornou-se preponderante e, ao final do ciclo do milho, retornou para uma condição semelhante à inicial.The availability of black oat residues, with high C:N ratio, leads to microbial immobilization of soil nitrogen, demanding special strategies to supply nitrogen to subsequent crops. The objective of this work was to evaluate shifts in microbial community structure due to the availability of black oat residues and nitrogen applications during the corn growing season. Soil (Paleudult samples were collected on the day of the corn seeding and after 46, 62, 88 and 112 days. Nitrogen fertilizer was applied 25 and 49 days after corn seeding. Changes in microbial community were assessed by microbial biomass carbon (C and nitrogen (N, ninhydrin-reactive N (N-Nin and carbohydrates (CHO

  8. Senescence of rin, rin/rin, rin/+ and +/+ tomato fruits

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    Zdravković Jasmina

    2004-01-01

    Full Text Available Ripening inhibitor (rin gene is a spontaneous recessive mutant which changes fruit ripening aspects (most important synthesis of carotene, especially lycopene. It also delays fruit senescence. Tomato is a vegetable crop with specific maturing climax (pik. Tomato genotypes homozygote for rin gene does not have this maturing climax, so the ethyl-ene production and red, lycopene colour does not appear. In order to research the maturing process material from the final tomato selection cycle we used: pure line S-49 (genetic constitution u/u, with uniform ripening, line hom 4 (rin/rin, homozygote with ripening inhibitor and hybrid combination 449 Fl (u/rin, heterozygote for ripening inhibitor. Fruits with uniform ripening and hybrids ripened simultaneously, while homozygote with rin gene did not ripe at all. Ethylene stimulates the appearance of yellow colour in rin fruits and the lycopene production. After treatment with ETEPHONE (0.1% (Ethylene fruits with uniform ripening, senescence more quickly, while hybrids senescence slowly. Rin homozygotes did not change colors, but the fruits senescence more quickly comparing to control. Ethylene treatment speeded the maturing and senescence process in tomato fruits. Extended maturing process as a result influence of rin gene, makes the new created hybrids a late maturing ones. On the other hand, the firmness of fruits is improved as well as the "shelf life", which enables longer transportation and storing, coordinated maturing according to market demands. The aim was to research the maturing according to market demands. The aim was to research the maturing process of rin heteroyzgote, and the reaction of some genotypes to treatment with RT-REL (Ethzlene in order to decrease extremely late maturing.

  9. Mapping and characterization of novel parthenocarpy QTLs in tomato.

    Science.gov (United States)

    Gorguet, Benoit; Eggink, Pieter Martijn; Ocaña, Juan; Tiwari, Aparna; Schipper, Danny; Finkers, Richard; Visser, Richard G F; van Heusden, Adriaan W

    2008-04-01

    Parthenocarpy is the development of the fruit in absence of pollination and/or fertilization. In tomato, parthenocarpy is considered as an attractive trait to solve the problems of fruit setting under unfavorable conditions. We studied the genetics of parthenocarpy in two different lines, IL5-1 and IVT-line 1, both carrying Solanum habrochaites chromosome segments. Parthenocarpy in IL5-1 is under the control of two QTLs, one on chromosome 4 (pat4.1) and one on chromosome 5 (pat5.1). IVT-line 1 also contains two parthenocarpy QTLs, one on chromosome 4 (pat4.2) and one on chromosome 9 (pat9.1). In addition, we identified one stigma exsertion locus in IL5-1, located on the long arm of chromosome 5 (se5.1). It is likely that pat4.1, from IL5-1 and pat4.2, from IVT-line 1, both located near the centromere of chromosome 4 are allelic. By making use of the microsynteny between tomato and Arabidopsis in this genetic region, we identified ARF8 as a potential candidate gene for these two QTLs. ARF8 is known to act as an inhibitor for further carpel development in Arabidopsis, in absence of pollination/fertilization. Expression of an aberrant form of the Arabidopsis ARF8 gene, in tomato, has been found to cause parthenocarpy. This candidate gene approach may lead to the first isolation of a parthenocarpy gene in tomato and will allow further use in several crop species.

  10. Inflorescence development in tomato: gene functions within a zigzag model.

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    Claire ePérilleux

    2014-03-01

    Full Text Available Tomato is a major crop plant and several mutants have been selected for breeding but also for isolating important genes that regulate flowering and sympodial growth. Besides, current research in developmental biology aims at revealing mechanisms that account for diversity in inflorescence architectures. We therefore found timely to review the current knowledge of the genetic control of flowering in tomato and to integrate the emerging network into modeling attempts. We developped a kinetic model of the tomato inflorescence development where each meristem was represented by its ‘vegetativeness’ (V, reflecting its maturation state towards flower initiation. The model followed simple rules: maturation proceeded continuously at the same rate in every meristem (dV; floral transition and floral commitment occurred at threshold levels of V; lateral meristems were initiated with a gain of V (ΔV relative to the V level of the meristem from which they derived. This last rule created a link between successive meristems and gave to the model its zigzag shape. We next exploited the model to explore the diversity of morphotypes that could be generated by varying dV and ΔV and matched them with existing mutant phenotypes. This approach, focused on the development of the primary inflorescence, allowed us to elaborate on the genetic regulation of the kinetic model of inflorescence development. We propose that the lateral inflorescence meristem fate in tomato is closer to an immature flower meristem than to the inflorescence meristem of Arabidopsis. In the last part of our paper, we extend our thought to spatial regulators that should be integrated in a next step for unraveling the relationships between the different meristems that participate to sympodial growth.

  11. Soil compost amendment enhances tomato (Solanum lycopersicum L.) quality.

    Science.gov (United States)

    Baldantoni, Daniela; Bellino, Alessandro; Alfani, Anna

    2016-09-01

    Tomato (Solanum lycopersicum L.) is one of the most important crops in the world and represents a key crop in southern Italy. With the aim to evaluate the nutritional characteristics of tomato fruits in relation to NPK and compost fertilisation, the concentrations of the main nutrients, toxic elements, primary metabolites and total phenols were determined in two varieties (Lido and San Marzano). Each variety was cultivated in a different experimental field, subjected to different agronomic techniques. Concentrations of toxic elements (Cd and Pb) were below the limits indicated by the EU Regulation (2011) in all the fruits analysed. Moreover, fruits obtained from San Marzano plants grown on organic amended soils showed a better overall quality than those obtained on mineral fertilised soil, being characterised by lower N (attributed to lower nitrate and nitrite concentrations), lower Cd, and higher soluble sugar concentrations. Higher concentrations of soluble sugars in fruits from organic amended soils were also observed in the Lido variety. The agricultural use of quality compost represents an effective strategy to obtain high quality products in an economically and environmentally sustainable way. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  12. A Reliable Wireless Control System for Tomato Hydroponics

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    Hirofumi Ibayashi

    2016-05-01

    Full Text Available Agricultural systems using advanced information and communication (ICT technology can produce high-quality crops in a stable environment while decreasing the need for manual labor. The system collects a wide variety of environmental data and provides the precise cultivation control needed to produce high value-added crops; however, there are the problems of packet transmission errors in wireless sensor networks or system failure due to having the equipment in a hot and humid environment. In this paper, we propose a reliable wireless control system for hydroponic tomato cultivation using the 400 MHz wireless band and the IEEE 802.15.6 standard. The 400 MHz band, which is lower than the 2.4 GHz band, has good obstacle diffraction, and zero-data-loss communication is realized using the guaranteed time-slot method supported by the IEEE 802.15.6 standard. In addition, this system has fault tolerance and a self-healing function to recover from faults such as packet transmission failures due to deterioration of the wireless communication quality. In our basic experiments, the 400 MHz band wireless communication was not affected by the plants’ growth, and the packet error rate was less than that of the 2.4 GHz band. In summary, we achieved a real-time hydroponic liquid supply control with no data loss by applying a 400 MHz band WSN to hydroponic tomato cultivation.

  13. A Reliable Wireless Control System for Tomato Hydroponics.

    Science.gov (United States)

    Ibayashi, Hirofumi; Kaneda, Yukimasa; Imahara, Jungo; Oishi, Naoki; Kuroda, Masahiro; Mineno, Hiroshi

    2016-05-05

    Agricultural systems using advanced information and communication (ICT) technology can produce high-quality crops in a stable environment while decreasing the need for manual labor. The system collects a wide variety of environmental data and provides the precise cultivation control needed to produce high value-added crops; however, there are the problems of packet transmission errors in wireless sensor networks or system failure due to having the equipment in a hot and humid environment. In this paper, we propose a reliable wireless control system for hydroponic tomato cultivation using the 400 MHz wireless band and the IEEE 802.15.6 standard. The 400 MHz band, which is lower than the 2.4 GHz band, has good obstacle diffraction, and zero-data-loss communication is realized using the guaranteed time-slot method supported by the IEEE 802.15.6 standard. In addition, this system has fault tolerance and a self-healing function to recover from faults such as packet transmission failures due to deterioration of the wireless communication quality. In our basic experiments, the 400 MHz band wireless communication was not affected by the plants' growth, and the packet error rate was less than that of the 2.4 GHz band. In summary, we achieved a real-time hydroponic liquid supply control with no data loss by applying a 400 MHz band WSN to hydroponic tomato cultivation.

  14. Multilocus sequence data reveal extensive departures from equilibrium in domesticated tomato (Solanum lycopersicum L.).

    Science.gov (United States)

    Labate, J A; Robertson, L D; Baldo, A M

    2009-09-01

    Limited genetic variation has been observed within tomato (Solanum lycopersicum L.), although no studies have extensively surveyed single nucleotide polymorphism (SNP) diversity among tomato landraces. We estimated intraspecific DNA sequence variation by analyzing 50 gene fragments (23.2 kb) per plant in a 31 plant diversity panel. The majority of loci (80%) were polymorphic with the minor allele at a frequency of 10% or less for most (141 of 155) SNPs. Mean diversity as estimated by theta and pi was approximately 1.5 SNPs per kb. Significant linkage disequilibrium was observed between 19% of locus pairs, and within-locus population recombination estimates were negligible. We also sequenced 43 gene fragments from wild tomato Solanum arcanum Peralta as an outgroup. Various statistical tests rejected a neutral equilibrium model of molecular evolution at 10 of 50 loci. Rare, highly diverged alleles were observed, involving at least seven tomato lines and five loci. Some of these may represent introgressions that originated both from natural hybridization with Solanum pimpinellifolium L. and from crosses with S. pimpinellifolium and additional wild relatives for crop improvement. The former was reported from classical field studies carried out by CM Rick; the latter has been extensively documented in the crop, particularly for transfer of disease resistance alleles. Extensive introgression and frequent bottlenecks within S. lycopersicum will pose a challenge to reconstructing the genetic bases of domestication and selection using methods that rely on patterns of molecular polymorphism.

  15. The Effect of Washing and Peeling on Reduction of Dithiocarbamates Residues in Cucumber and Tomato

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    Mohammad Reza Mehrasebi

    2016-03-01

    Full Text Available Background: Dithiocarbamates, the main group of fungicides, are used to control about 400 pathogens in more than 70 crops. These pesticides are widely applied to crops including potato, cereal, apple, pear and leafy vegetables throughout the world since 1960. From the late 1980s, using these fungicides has caused much debate among regulators about their long-term effects on consumers and occupational users. Method: In this study the residues of Dithiocarbamates in cucumber and tomato using the colorimetric method (Keppel method was measured. Respectively 80 and 45 samples of greenhouse cucumber and tomato were collected from Zanjan vegetables center in autumns and winter 2013. The samples were analyzed in 4 treatments of: unwashed, washing with water, washing whit detergent and peeling. Result: The results showed that the average concentration of Dithiocarbamates residues in unwashed greenhouse cucumber and tomatoes were 384.5 µg/kg and 65 µg/kg respectively. 35% and 5% of unwashed and water washed cucumber and tomato samples (respectively had higher Dithiocarbamates residue than MRL recommended by Institute of Standards and Industrial Research of Iran (0.5mg/kg. Conclusion: The treatments of washing and peeling had significant effect on the reduction of Dithiocarbamates residues in the all samples.

  16. Flight movement and spatial distribution of immunomarked thrips in onion, potato, and tomato

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    Flávio Lemes Fernandes

    2015-05-01

    Full Text Available The objective of this work was to evaluate the spatial distribution of thrips in different crops, and the correlation between meterological parameters and the flight movements of this pest, using immunomarking. The experiment was conducted in cultivated areas, with tomato (Solanum lycopersicum, potato (Solanum tuberosum, and onion (Allium cepa; and non-cultivated areas, with weedy plants. The areas with tomato (100 days, potato (20 days, and weeds were sprayed with casein, albumin, and soy milk, respectively, to mark adult thrips; however, the areas with onion (50 days and tomato (10 days were not sprayed. Thrips were captured with georeferenced blue sticky traps, transferred into tubes, and identified by treatment area with the Elisa test. The dependence between the samples and the capture distance was determined using geostatistics. Meteorlogical parameters were correlated with thrips density in each area. The three protein types used for immunomarking were detected in different proportions in the thrips. There was a correlation between casein-marked thrips and wind speed. The thrips flew a maximum distance of 3.5 km and dispersed from the older (tomato to the younger crops (potato. The immunomarking method is efficient to mark large quantities of thrips.

  17. Growth and conversion of solar energy of grafted tomato plants under protected cultivation

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    Tiago Pedó

    2015-07-01

    Full Text Available The grafting technique favors cultivation tomato under conditions environment adverse, being the effects on the physiology of scarce plants. Thus, this study aimed to evaluate the growth and solar energy conversion efficiency in grafted tomato and not grafted in greenhouse. The grafting was performed by grafting of cleft and the treatments consisted of tomato plants grafted on hybrid Kaguemusha® and not grafted. The samples for growth analysis were performed at intervals of fourteen days after transplanting (DAT by the end of the crop cycle. At each harvest, plants were separated into organs, being determined to total dry matter (Wt, rates of dry matter production (Ct and relative growth (Rw, net assimilation (Ea, leaf area index (L, growth rate, leaf area (Ca, relative growth of leaf area (Ra, leaf area ratio (Fa, leaf weight (Fw, specific leaf area (Sa, conversion efficiency solar energy (? and assimilation rate of fruit (Efr. From the analysis of data growth, the plants grafted on the hybrid Kaguemusha® had higher Wt, Ct, Rw, Ea and ? compared to non-grafted that showed a high Fa and Fw. Therefore, the stress caused by grafting did not affect the growth at the end of the development cycle of tomato plants, being important feature to keep the crop yield

  18. Tomato fruits: a good target for iodine biofortification

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    Claudia eKiferle

    2013-06-01

    Full Text Available Iodine is a trace element that is fundamental for human health: its deficiency affects about two billion people worldwide. Fruits and vegetables are usually poor sources of iodine; however plants can accumulate iodine if it is either present or exogenously administered to the soil. The biofortification of crops with iodine has therefore been proposed as a strategy for improving human nutrition. A greenhouse pot experiment was carried out to evaluate the possibility of biofortifying tomato fruits with iodine. Increasing concentrations of iodine supplied as KI or KIO3 were administered to plants as root treatments and the iodine accumulation in fruits was measured. The influences of the soil organic matter content or the nitrate level in the nutritive solution were analyzed. Finally, yield and qualitative properties of the biofortified tomatoes were considered, as well as the possible influence of fruit storage and processing on the iodine content. Results showed that the use of both the iodized salts induced a significant increase in the fruit’s iodine content in doses that did not affect plant growth and development. The final levels ranged from a few mg up to 10 mg iodine kg-1 fruit fresh weight and are more than adequate for a biofortification program, since 150 µg iodine per day is the recommended dietary allowance for adults. In general, the iodine treatments scarcely affected fruit appearance and quality, even with the highest concentrations applied. In contrast, the use of KI in plants fertilized with low doses of nitrate induced moderate phytotoxicity symptoms. Organic matter-rich soils improved the plant’s health and production, with only mild reductions in iodine stored in the fruits. Finally, a short period of storage at room temperature or a 30-minute boiling treatment did not reduce the iodine content in the fruits, if the peel was maintained. All these results suggest that tomato is a particularly suitable crop for iodine

  19. Shoot and root dry weight in drought exposed tomato populations

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    Brdar-Jokanović Milka

    2014-01-01

    Full Text Available This research was conducted with the aim to among forty-one tested tomato (Lycopersicon esculentum Mill populations distinguish those tolerant to limited water supply. Tolerance assessments were performed by using sixteen drought stress selection indices calculated on the basis of tomato shoot and root dry weight yields determined at water stress and non-stress conditions. Populations were differentiated in groups using the method of cluster analysis. The pot experiment was set in controlled greenhouse conditions and comprised optimally irrigated control and drought treatment (35.0 and 20.9% volumetric soil water content, respectively, imposed at the phase of intensive vegetative growth. The experiment was conducted at the Institute for Vegetable Crops in Smederevska Palanka, Serbia. The analyzed tomatoes exhibited significant differences in terms of response to limited irrigation, which had more pronounced effect on shoot dry weight than on the roots (average decrease of 64.4 and 35.7%, respectively. Consequently, root fraction in the total dry weight increased at drought for 68.2% on average. Shoot and root dry weights were positively correlated at optimal irrigation but not in drought, implying genotypic differences in terms of root adjustments to stress conditions. As for the calculated selection indices, substantial variation was found among the populations enabling their ranking in terms of drought tolerance. Since ranking was not the same in all cases, clustering the populations was performed taking into account all sixteen selection indices. The results of this analysis indicate that populations designated with numbers 126, 124, 131, 125, 128, 105, 101, 138, 110, 132 and 109 in Institute for Vegetable Crops germplasm collection exhibit satisfactory level of drought tolerance at vegetative phase and therefore may be used as parents in breeding programs. [Projekat Ministarstva nauke Republike Srbije, br. TR 31005 i br. TR 31059

  20. Genome-wide microarray analysis of tomato roots showed defined responses to iron deficiency

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    Zamboni Anita

    2012-03-01

    Full Text Available Abstract Background Plants react to iron deficiency stress adopting different kind of adaptive responses. Tomato, a Strategy I plant, improves iron uptake through acidification of rhizosphere, reduction of Fe3+ to Fe2+ and transport of Fe2+ into the cells. Large-scale transcriptional analyses of roots under iron deficiency are only available for a very limited number of plant species with particular emphasis for Arabidopsis thaliana. Regarding tomato, an interesting model species for Strategy I plants and an economically important crop, physiological responses to Fe-deficiency have been thoroughly described and molecular analyses have provided evidence for genes involved in iron uptake mechanisms and their regulation. However, no detailed transcriptome analysis has been described so far. Results A genome-wide transcriptional analysis, performed with a chip that allows to monitor the expression of more than 25,000 tomato transcripts, identified 97 differentially expressed transcripts by comparing roots of Fe-deficient and Fe-sufficient tomato plants. These transcripts are related to the physiological responses of tomato roots to the nutrient stress resulting in an improved iron uptake, including regulatory aspects, translocation, root morphological modification and adaptation in primary metabolic pathways, such as glycolysis and TCA cycle. Other genes play a role in flavonoid biosynthesis and hormonal metabolism. Conclusions The transcriptional characterization confirmed the presence of the previously described mechanisms to adapt to iron starvation in tomato, but also allowed to identify other genes potentially playing a role in this process, thus opening new research perspectives to improve the knowledge on the tomato root response to the nutrient deficiency.

  1. Genome-wide analysis of histone modifiers in tomato: gaining an insight into their developmental roles

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    Aiese Cigliano Riccardo

    2013-01-01

    Full Text Available Abstract Background Histone post-translational modifications (HPTMs including acetylation and methylation have been recognized as playing a crucial role in epigenetic regulation of plant growth and development. Although Solanum lycopersicum is a dicot model plant as well as an important crop, systematic analysis and expression profiling of histone modifier genes (HMs in tomato are sketchy. Results Based on recently released tomato whole-genome sequences, we identified in silico 32 histone acetyltransferases (HATs, 15 histone deacetylases (HDACs, 52 histone methytransferases (HMTs and 26 histone demethylases (HDMs, and compared them with those detected in Arabidopsis (Arabidopsis thaliana, maize (Zea mays and rice (Oryza sativa orthologs. Comprehensive analysis of the protein domain architecture and phylogeny revealed the presence of non-canonical motifs and new domain combinations, thereby suggesting for HATs the existence of a new family in plants. Due to species-specific diversification during evolutionary history tomato has fewer HMs than Arabidopsis. The transcription profiles of HMs within tomato organs revealed a broad functional role for some HMs and a more specific activity for others, suggesting key HM regulators in tomato development. Finally, we explored S. pennellii introgression lines (ILs and integrated the map position of HMs, their expression profiles and the phenotype of ILs. We thereby proved that the strategy was useful to identify HM candidates involved in carotenoid biosynthesis in tomato fruits. Conclusions In this study, we reveal the structure, phylogeny and spatial expression of members belonging to the classical families of HMs in tomato. We provide a framework for gene discovery and functional investigation of HMs in other Solanaceae species.

  2. How and why does tomato accumulate a large amount of GABA in the fruit?

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    Mariko eTakayama

    2015-08-01

    Full Text Available γ-Aminobutyric acid (GABA has received much attention as a health-promoting functional compound, and several GABA-enriched foods have been commercialized. In higher plants, GABA is primarily metabolized via a short pathway called the GABA shunt. The GABA shunt bypasses two steps (the oxidation of α-ketoglutarate to succinate of the tricarboxylic acid (TCA cycle via reactions catalysed by three enzymes: glutamate decarboxylase (GAD, GABA transaminase (GABA-T and succinic semialdehyde dehydrogenase (SSADH. The GABA shunt plays a major role in primary carbon and nitrogen metabolism and is an integral part of the TCA cycle under stress and non-stress conditions. Tomato is one of the major crops that accumulate a relatively high level of GABA in its fruits. The GABA levels in tomato fruits dramatically change during fruit development; the GABA levels increase from flowering to the mature green stage and then rapidly decrease during the ripening stage. Although GABA constitutes up to 50% of the free amino acids at the mature green stage, the molecular mechanism of GABA accumulation and the physiological function of GABA during tomato fruit development remain unclear. In this review, we summarize recent studies of GABA accumulation in tomato fruits and discuss the potential biological roles of GABA in tomato fruit development.

  3. Host-Induced Silencing of Pathogenicity Genes Enhances Resistance to Fusarium oxysporum Wilt in Tomato.

    Science.gov (United States)

    Bharti, Poonam; Jyoti, Poonam; Kapoor, Priya; Sharma, Vandana; Shanmugam, V; Yadav, Sudesh Kumar

    2017-08-01

    This study presents a novel approach of controlling vascular wilt in tomato by RNAi expression directed to pathogenicity genes of Fusarium oxysporum f. sp. lycopersici. Vascular wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici leads to qualitative and quantitative loss of the crop. Limitation in the existing control measures necessitates the development of alternative strategies to increase resistance in the plants against pathogens. Recent findings paved way to RNAi, as a promising method for silencing of pathogenicity genes in fungus and provided effective resistance against fungal pathogens. Here, two important pathogenicity genes FOW2, a Zn(II)2Cys6 family putative transcription regulator, and chsV, a putative myosin motor and a chitin synthase domain, were used for host-induced gene silencing through hairpinRNA cassettes of these genes against Fusarium oxysporum f. sp. lycopersici. HairpinRNAs were assembled in appropriate binary vectors and transformed into tomato plant targeting FOW2 and chsV genes, for two highly pathogenic strains of Fusarium oxysporum viz. TOFOL-IHBT and TOFOL-IVRI. Transgenic tomatoes were analyzed for possible attainment of resistance in transgenic lines against fungal infection. Eight transgenic lines expressing hairpinRNA cassettes showed trivial disease symptoms after 6-8 weeks of infection. Hence, the host-induced posttranscriptional gene silencing of pathogenicity genes in transgenic tomato plants has enhanced their resistance to vascular wilt disease caused by Fusarium oxysporum.

  4. Effects of Fertilization on Tomato Growth and Soil Enzyme Activity

    Science.gov (United States)

    Mu, Zhen; Hu, Xue-Feng; Cheng, Chang; Luo, Zhi-qing

    2015-04-01

    To study the effects of different fertilizer applications on soil enzyme activity, tomato plant growth and tomato yield and quality, a field experiment on tomato cultivation was carried out in the suburb of Shanghai. Three fertilizer treatments, chemical fertilizer (CF) (N, 260 g/kg; P, 25.71g/kg; K, 83.00g/kg), rapeseed cake manure (CM) (N, 37.4 g/kg; P, 9.0 g/kg; K, 8.46 g/kg), crop-leaf fermenting manure (FM) (N, 23.67 g/kg; P, 6.39 g/kg; K 44.32 g/kg), and a control without using any fertilizers (CK), were designed. The total amounts of fertilizer application to each plot for the CF, CM, FM and CK were 0.6 kg, 1.35 kg, 3.75 kg and 0 kg, respectively, 50% of which were applied as base fertilizer, and another 50% were applied after the first fruit picking as top dressing. Each experimental plot was 9 m2 (1 m × 9 m) in area. Each treatment was replicated for three times. No any pesticides and herbicides were applied during the entire period of tomato growth to prevent their disturbance to soil microbial activities. Soil enzyme activities at each plot were constantly tested during the growing period; the tomato fruit quality was also constantly analyzed and the tomato yield was calculated after the final harvesting. The results were as follows: (1) Urease activity in the soils treated with the CF, CM and FM increased quickly after applying base fertilizer. That with the CF reached the highest level. Sucrase activity was inhibited by the CF and CM to some extent, which was 32.4% and 11.2% lower than that with the CK, respectively; while that with the FM was 15.7% higher than that with the CK. Likewise, catalase activity with the CF increased by 12.3% - 28.6%; that with the CM increased by 87.8% - 95.1%; that with the FM increased by 86.4% - 93.0%. Phosphatase activity with the CF increased rapidly and reached a maximum 44 days after base fertilizer application, and then declined quickly. In comparison, that with the CM and FM increased slowly and reached a maximum

  5. Long-life tomato cultivars growing under the hydroponic Nutrient Film Technique

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    Gualberto Ronan

    2002-01-01

    Full Text Available Farmers are interested in hydroponic tomato cultivation because of the high demand of high quality products by the consumers. Three trials here carried out under greenhouse conditions in Marília, SP, Brazil, using the hydroponic nutrient film technique, in order to evaluate yield and quality of long-life salad tomato (Lycopersicon esculentum varieties, during 1998 and 1999. Four varieties (Carmen, Diva, Graziela, and Vita were tested using a randomized block design, with six replications. The variety x crop season interaction effect was significant, meaning that varieties have distinct performances during the different cropping seasons. The Vita variety presented commercial valid yield, with mean fruit weights higher than the other varieties.

  6. Yield and Quality Responses of Selected Solanaceous Vegetable Crops to Potassium Fertilization

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Over a period of two years, field experiments were conducted on two silty loam soils grown with four solanaceous vegetable crops of eggplant (var. serpentinum Bailey), tomato (var. commune Bailey), sweet pepper (var. grossum Bailey) and chilli (var. lengum Bailey), respectively. Each experiment included four treatments with from low to high doses, 0~450 kg ha-1 for eggplant, tomato and sweet pepper, and 0~270 kg ha- 1 for chilli, of K fertilizers in the form of sulfate of potash (SOP) applied together with N and P fertilizers. One CK treatment without K, N and P fertilizers applied and one treatment of K fertilizer in the form of muriate of potash (MOP) applied at the high level (450 kg ha-i) together with N and P fertilizers were included in the experiments of eggplant, in order to compare the effects of SOP and MOP. The fruit yields of the tested crops increased significantly with the increasing rate of K application. The crops supplied with K fertilizers yielded more stably as the CV% of their yields decreased with the rate of K application. The dry matter and vitamin C contents in fruits of tomato, sweet pepper and chilli, and the sugar content and the titratable acidity level of tomato fruits were increased, and the S/A ratio (ratio of sugar content to titratable acidity) of tomato fruits were decreased by K fertilization, indicating that K fertilization could improve the fruit quality of the solanaceous vegetable crops. However, the high rate of K fertilizer might lower the dry matter and vitamin C contents of tomato fruits and sweet pepper fruits. SOP was more effective than MOP in increasing the yield and quality of eggplant fruits at the high fertilization rate; therefore, the choice of applying SOP may be better for high levels of K fertilization.

  7. Propriedades químicas de um Cambissolo Húmico sob preparo convencional e semeadura direta após seis anos de cultivo Chemical properties of a Humic Cambisol under conventional tillage and no-tillage after six years of cropping

    Directory of Open Access Journals (Sweden)

    Jaime Antonio de Almeida

    2005-06-01

    do Mg em profundidade na semeadura direta. Nesse sistema, constatou-se acúmulo de K e P nos primeiros centímetros superficiais do solo.In the conventional tillage system the soil is mobilized, incorporating and distributing crop residues and fertilizers in the arable layer. In no-tillage systems, however, where no soil mobilization occurs, lime and fertilizers are applied on the soil surface, which combined with the maintenance of crop residues cover favor the formation of a gradient of nutrient and organic matter concentration in the superficial soil layer. In the present study it was evaluated the effect of these two tillage systems on the chemical attributes of a Humic Cambisol, in Lages County, State of Santa Catarina, Brazil, in 2001, six years after the soil pH had been corrected by liming. The data were compared with those of a soil from a nearby native field. The following soil treatments with four replications were used: conventional tillage with one plowing and two diskings in crop rotation (PCR and in crop succession (PCS, no-tillage in crop rotation (SDR and in crop succession (SDS, and a native field (CN. The cropping sequence comprised of bean/fallow/corn/fallow/soybean/fallow was adopted in the PCR, whereas the corn/fallow sequence was adopted in the PCS system. In SDR the sequence was bean/oat/corn/fodder radish/soybean/vetch and, whereas corn/vetch was cultivated in the SDS. In April 2001, organic carbon, extractable phosphorus, exchangeable potassium, calcium, magnesium and aluminum (Al3+, potential acidity (H + Al, cation exchange capacity (CEC, and pH in water were analyzed at the depths 0-2.5; 2.5-5.0; 5.0-10.0; 10.0-15.0; 15.0-20.0 and 20.0-30.0 cm. Despite the insignificance of the differences, a tendency to a slight pH reduction and H + Al increase was observed in most superficial layers under no-tillage compared to the conventional tillage system. Significant increases of organic carbon in the no-tillage system seem to be responsible for

  8. Manejo de plantas daninhas na cultura do tomateiro Weed management in tomato

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    C.P. Ronchi

    2010-01-01

    Full Text Available O tomate (Lycopersicon esculentum é a segunda hortaliça em importância no Brasil. O controle de plantas daninhas na cultura representa um dos principais componentes do custo de produção, sendo imprescindível para a obtenção de produtividades elevadas e de frutos de qualidade. Neste texto são apresentados de forma detalhada os efeitos adversos da interferência das plantas daninhas sobre a cultura, tanto daquele cultivado a partir de mudas transplantadas como de semeadura direta. Também são discutidos, para diferentes situações de cultivo, aspectos sobre o período crítico de competição das plantas daninhas durante o ciclo da cultura. Por fim, são apresentadas as principais estratégias utilizadas para o controle de plantas daninhas na cultura do tomateiro, por meio de medidas preventivas, culturais, mecânicas e químicas, com base nas informações atualmente disponíveis sobre esse tema.Tomato (Lycopersicon esculentum is the second most important vegetable grown in Brazil. Weed control in tomato culture is one of the main components in crop production costs. However, weed management is indispensable to achieve both high yields and fruit quality. A detailed discussion is presented in this work concerning the adverse effects of weed interference in the crop, both grown from transplanted seedlings and field-sown tomato. Aspects related to the critical period of weed competition during the crop cycle for both growing conditions are also discussed. Finally, the major strategies used for weed management in tomato crops are also presented, such as preventive, cultural, mechanic, and chemical control measures, based on the literature currently available.

  9. First Report of Tomato torrado virus Infecting Tomato in Colombia

    NARCIS (Netherlands)

    Verbeek, M.; Dullemans, A.M.

    2012-01-01

    Tomato (Solanum lycopersicum L.) plants grown in plastic greenhouses near Villa de Leyva, northeast of Bogota, Colombia showed necrotic spots on the leaves in September 2008. Initial symptoms were necrosis beginning at the base of leaflets that were surrounded by yellow areas. These symptoms resembl

  10. First Report of Tomato torrado virus Infecting Tomato in Colombia

    NARCIS (Netherlands)

    Verbeek, M.; Dullemans, A.M.

    2012-01-01

    Tomato (Solanum lycopersicum L.) plants grown in plastic greenhouses near Villa de Leyva, northeast of Bogota, Colombia showed necrotic spots on the leaves in September 2008. Initial symptoms were necrosis beginning at the base of leaflets that were surrounded by yellow areas. These symptoms resembl

  11. Crop residue management in arable cropping systems under a temperate climate. Part 2: Soil physical properties and crop production. A review

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    Hiel, MP.

    2016-01-01

    Full Text Available Introduction. Residues of previous crops provide a valuable amount of organic matter that can be used either to restore soil fertility or for external use. A better understanding of the impact of crop residue management on the soil-water-plant system is needed in order to manage agricultural land sustainably. This review focuses on soil physical aspects related to crop residue management, and specifically on the link between soil structure and hydraulic properties and its impact on crop production. Literature. Conservation practices, including crop residue retention and non-conventional tillage, can enhance soil health by improving aggregate stability. In this case, water infiltration is facilitated, resulting in an increase in plant water availability. Conservation practices, however, do not systematically lead to higher water availability for the plant. The influence of crop residue management on crop production is still unclear; in some cases, crop production is enhanced by residue retention, but in others crop residues can reduce crop yield. Conclusions. In this review we discuss the diverse and contrasting effects of crop residue management on soil physical properties and crop production under a temperate climate. The review highlights the importance of environmental factors such as soil type and local climatic conditions, highlighting the need to perform field studies on crop residue management and relate them to specific pedo-climatic contexts.

  12. Screening South African potato, tomato and wheat cultivars for five carotenoids

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    Charlotte S. Mashaba

    2011-09-01

    Full Text Available In South Africa malnutrition is of great concern. Vitamin A deficiency is one of the leading causes of infections as a result of micronutrient malnutrition. Although supplementation and food fortification programmes exist, these either are not available or are unaffordable to communities in remote rural areas. The selection of crops that are naturally rich in provitamin A (β-carotene and other carotenoids that can be recommended to small-scale farmers for breeding and for food production, could be an effective way to address vitamin A deficiencies and associated diseases. The aim of this study was to profile two cultivars each of potato, tomato, bread wheat and durum wheat, which are highly consumed crops in South Africa, for their carotenoid content using high-performance liquid chromatography. To this effect, reliable extraction and quantification of five carotenoids – lutein, zeaxanthin, canthaxanthin, β-carotene and lycopene – were performed for these crops. Lutein and zeaxanthin were found to be the major carotenoids in potato, whilst lycopene was the major carotenoid in tomato. In durum wheat, only lutein and zeaxanthin were identified whilst bread wheat contained lutein, zeaxanthin and β-carotene. The methodology used proved to be robust and suitable to screen a large number of potato, tomato and wheat cultivars for their carotenoid content.

  13. Outcrossing potential between 11 important genetically modified crops and the Chilean vascular flora.

    Science.gov (United States)

    Sánchez, Miguel A; Cid, Pablo; Navarrete, Humberto; Aguirre, Carlos; Chacón, Gustavo; Salazar, Erika; Prieto, Humberto

    2016-02-01

    The potential impact of genetically modified (GM) crops on biodiversity is one of the main concerns in an environmental risk assessment (ERA). The likelihood of outcrossing and pollen-mediated gene flow from GM crops and non-GM crops are explained by the same principles and depend primarily on the biology of the species. We conducted a national-scale study of the likelihood of outcrossing between 11 GM crops and vascular plants in Chile by use of a systematized database that included cultivated, introduced and native plant species in Chile. The database included geographical distributions and key biological and agronomical characteristics for 3505 introduced, 4993 native and 257 cultivated (of which 11 were native and 246 were introduced) plant species. Out of the considered GM crops (cotton, soya bean, maize, grape, wheat, rice, sugar beet, alfalfa, canola, tomato and potato), only potato and tomato presented native relatives (66 species total). Introduced relative species showed that three GM groups were formed having: a) up to one introduced relative (cotton and soya bean), b) up to two (rice, grape, maize and wheat) and c) from two to seven (sugar beet, alfalfa, canola, tomato and potato). In particular, GM crops presenting introduced noncultivated relative species were canola (1 relative species), alfalfa (up to 4), rice (1), tomato (up to 2) and potato (up to 2). The outcrossing potential between species [OP; scaled from 'very low' (1) to 'very high' (5)] was developed, showing medium OPs (3) for GM-native relative interactions when they occurred, low (2) for GMs and introduced noncultivated and high (4) for the grape-Vitis vinifera GM-introduced cultivated interaction. This analytical tool might be useful for future ERA for unconfined GM crop release in Chile.

  14. Genome-wide association mapping in tomato (Solanum lycopersicum) is possible using genome admixture of Solanum lycopersicum var. cerasiforme.

    Science.gov (United States)

    Ranc, Nicolas; Muños, Stephane; Xu, Jiaxin; Le Paslier, Marie-Christine; Chauveau, Aurélie; Bounon, Rémi; Rolland, Sophie; Bouchet, Jean-Paul; Brunel, Dominique; Causse, Mathilde

    2012-08-01

    Genome-wide association mapping is an efficient way to identify quantitative trait loci controlling the variation of phenotypes, but the approach suffers severe limitations when one is studying inbred crops like cultivated tomato (Solanum lycopersicum). Such crops exhibit low rates of molecular polymorphism and high linkage disequilibrium, which reduces mapping resolution. The cherry type tomato (S. lycopersicum var. cerasiforme) genome has been described as an admixture between the cultivated tomato and its wild ancestor, S. pimpinellifolium. We have thus taken advantage of the properties of this admixture to improve the resolution of association mapping in tomato. As a proof of concept, we sequenced 81 DNA fragments distributed on chromosome 2 at different distances in a core collection of 90 tomato accessions, including mostly cherry type tomato accessions. The 81 Sequence Tag Sites revealed 352 SNPs and indels. Molecular diversity was greatest for S. pimpinellifolium accessions, intermediate for S. l. cerasiforme accessions, and lowest for the cultivated group. We assessed the structure of molecular polymorphism and the extent of linkage disequilibrium over genetic and physical distances. Linkage disequilibrium decreased under r(2) = 0.3 within 1 cM, and minimal estimated value (r(2) = 0.13) was reached within 20 kb over the physical regions studied. Associations between polymorphisms and fruit weight, locule number, and soluble solid content were detected. Several candidate genes and quantitative trait loci previously identified were validated and new associations detected. This study shows the advantages of using a collection of S. l. cerasiforme accessions to overcome the low resolution of association mapping in tomato.

  15. Carbon monoxide formation in tomatoes

    Energy Technology Data Exchange (ETDEWEB)

    Gladon, R.J.; Staby, G.L.

    1979-01-01

    Carbon monoxide (CO) is not emanated to any large extent from tomato fruits (Lycopersicon esculentum, Mill. cvs. Rutgers and Ohio MR-13), but is retained within the internal atmosphere. CO is found during all stages of fruit development, but no set pattern of CO concentration is evident.

  16. Can the Tomato Prevent Cancer?

    Institute of Scientific and Technical Information of China (English)

    王建明

    2009-01-01

    难度:★★★★☆字数:368建议时间:5分钟Apurple tomato genetically engineered to con-tain nutrients more commonly seen in dark berries helped prevent cancer in mice,British researchers said on Sunday.The finding,published in

  17. Biological characteristics of tomato mild mottle potyvirus isolated ...

    African Journals Online (AJOL)

    Biological characteristics of tomato mild mottle potyvirus isolated from tomato and thorn ... (Datura stramonium) and tomato (Lycopersicon lycopersicum (L.) Karst. ... Solanum demissum L., while the PVY isolate infected Chenopodium quinoa ...

  18. Phytotoxicity of tolylfluanid in tomatoes.

    Science.gov (United States)

    Gielen, S; Vogels, L; Seels, B; Aerts, R

    2006-01-01

    The use of Euparen Multi (tolylfluanid) for controlling Botrytis cinerea in tomatoes has been decreased the last decade for several reasons. Because of the lack of different fungicides with a good efficacy it is important that growers can use different fungicides to prevent development of resistance of Botrytis cinerea against many fungicides. Tolylfluanid has negative side effects on some insect populations that are used for biological control. It is known that Euparen Multi and Euparen can have a negative effect on some predatory mites (Schmidt and Zeller, 1998) such as Phytoseiulus persimilis and some parasitic wasps like Encarsia formosa, Eretmocerus eremicus, Diglyphus isaea and Dacnusa sibirica. Recently investigation indicates that this fungicide is harmless for the predatory bug Macrolophus caliginosus (Biobest, 2006) frequently used in the cultivation of tomatoes as a predator for whitefly (Trialeurodes vaporariorum). To investigate if tolylfluanid has a phytotoxic effect on tomato plants an experiment was performed. Young tomato plants were used, who are more sensitive. These plants were subdivided in different groups, from which each one was sprayed with a different concentration of tolylfluanid. The highest concentrations of tolylfluanid were used to stimulate the visibility of the possible phytotoxic effects. Results of this experiment demonstrate that there wasn't a difference between the different groups that were sprayed with tolylfluanid or the control group. This indicates that tolylfluanid doesn't seem to be phytotoxic. It is also important to mention that this experiment was done in the fall when the intensity of the sunlight was decreasing. There still exists the possibility that extreme irradiation in combination with tolylfluanid can provoke a phototoxic effect on young tomato plants.

  19. Eficiência no uso da água e interferência de plantas daninhas no meloeiro cultivado nos sistemas de plantio direto e convencional Water use efficiency and weed interference in melon crop under conventional and no-tillage systems

    Directory of Open Access Journals (Sweden)

    T.M.S. Teófilo

    2012-09-01

    filme de polietileno nos dois sistemas de plantio ou com cobertura morta no plantio direto aumentou a eficiência no uso da água em relação ao solo sem cobertura. No tratamento sem capinas no sistema de plantio convencional, além da perda total na produtividade comercial, a interferência das plantas daninhas aumentou o consumo de água em 9,6%.The objective of this work was to evaluate the effect of the conventional and no-tillage systems and weed management strategies on water use efficiency in melon crop (Cucumis melo. Thus, an experiment was carried out in a randomized block design in split-plots in Mossoró-RN. The plots were constituted of two tillage systems (conventional and no-tillage and the sub-plots of three weed management systems (plastic mulch, weeding, and no weeding. Density and dry weight of the weeds at 30 days after transplanting, commercial and total yield and daily consumption of water were evaluated. Irrigation management was performed by the characteristic soil water curve for each cropping system at 15 and 30 cm of depth and water control by daily reading a set of tensiometers installed to keep the soil above 75% of field capacity. Water use efficiency (USA, given in kg of fruit per m3, was determined based on the crop's yield and water consumption. It was found that the no-tillage system reduced weed density and dry matter in 86.7 and 61%, respectively, compared to conventional tillage, and weed interference reduced commercial yield by 100% under conventional tillage and 36.5% under no-tillage. Soil with plastic mulch under no-tillage and conventional systems, and straw mulching under no-tillage reduced water consumption by 23% (388.8 m3 ha-1, 21% (363.0 m3 ha-1 and 13% (215.0 m3 ha-1, respectively, compared to the weeding treatment under conventional tillage. Soil with plastic mulch under the two tillage systems, or straw mulching under no-tillage increased the efficiency of water use from the ground without cover. In the no

  20. Ultrasound assisted extraction and characterization of pectin from tomato waste.

    Science.gov (United States)

    Grassino, Antonela Ninčević; Brnčić, Mladen; Vikić-Topić, Dražen; Roca, Sunčica; Dent, Maja; Brnčić, Suzana Rimac

    2016-05-01

    Pectin was extracted from tomato waste using two different extraction methods to assess its potential u