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Sample records for controls regulatory tolerance

  1. The immunology of pregnancy: regulatory T cells control maternal immune tolerance toward the fetus.

    Science.gov (United States)

    La Rocca, Claudia; Carbone, Fortunata; Longobardi, Salvatore; Matarese, Giuseppe

    2014-11-01

    Establishment and maintenance of pregnancy represents a challenge for the maternal immune system since it has to defend against pathogens and tolerate paternal alloantigens expressed in fetal tissues. Regulatory T (Treg) cells, a subset of suppressor CD4(+) T cells, play a dominant role in the maintenance of immunological self-tolerance by preventing immune and autoimmune responses against self-antigens. Although localized mechanisms contribute to fetal evasion from immune attack, in the last few years it has been observed that Treg cells are essential in promoting fetal survival avoiding the recognition of paternal semi-allogeneic tissues by maternal immune system. Several functional studies have shown that unexplained infertility, miscarriage and pre-clampsia are often associated with deficit in Treg cell number and function while normal pregnancy selectively stimulates the accumulation of maternal forkhead-box-P3(+) (FoxP3(+)) CD4(+) Treg cells with fetal specificity. Some papers have been reported that the number of Treg cells persists at elevated levels long after delivery developing an immune regulatory memory against father's antigens, moreover these memory Treg cells rapidly proliferate during subsequent pregnancies, however, on the other hand, there are several evidence suggesting a clear decline of Treg cells number after delivery. Different factors such as cytokines, adipokines, pregnancy hormones and seminal fluid have immunoregulatory activity and influence the success of pregnancy by increasing Treg cell number and activity. The development of strategies capable of modulating immune responses toward fetal antigens through Treg cell manipulation, could have an impact on the induction of tolerance against fetal antigens during immune-mediated recurrent abortion.

  2. Regulatory focus affects physician risk tolerance.

    Science.gov (United States)

    Veazie, Peter J; McIntosh, Scott; Chapman, Benjamin P; Dolan, James G

    2014-01-01

    Risk tolerance is a source of variation in physician decision-making. This variation, if independent of clinical concerns, can result in mistaken utilization of health services. To address such problems, it will be helpful to identify nonclinical factors of risk tolerance, particularly those amendable to intervention-regulatory focus theory suggests such a factor. This study tested whether regulatory focus affects risk tolerance among primary care physicians. Twenty-seven primary care physicians were assigned to promotion-focused or prevention-focused manipulations and compared on the Risk Taking Attitudes in Medical Decision Making scale using a randomization test. Results provide evidence that physicians assigned to the promotion-focus manipulation adopted an attitude of greater risk tolerance than the physicians assigned to the prevention-focused manipulation (p = 0.01). The Cohen's d statistic was conventionally large at 0.92. Results imply that situational regulatory focus in primary care physicians affects risk tolerance and may thereby be a nonclinical source of practice variation. Results also provide marginal evidence that chronic regulatory focus is associated with risk tolerance (p = 0.05), but the mechanism remains unclear. Research and intervention targeting physician risk tolerance may benefit by considering situational regulatory focus as an explanatory factor.

  3. Fault Tolerant Feedback Control

    DEFF Research Database (Denmark)

    Stoustrup, Jakob; Niemann, H.

    2001-01-01

    An architecture for fault tolerant feedback controllers based on the Youla parameterization is suggested. It is shown that the Youla parameterization will give a residual vector directly in connection with the fault diagnosis part of the fault tolerant feedback controller. It turns out...... that there is a separation be-tween the feedback controller and the fault tolerant part. The closed loop feedback properties are handled by the nominal feedback controller and the fault tolerant part is handled by the design of the Youla parameter. The design of the fault tolerant part will not affect the design...... of the nominal feedback con-troller....

  4. Fault Tolerant Control Systems

    DEFF Research Database (Denmark)

    Bøgh, S. A.

    was to avoid a total close-down in case of the most likely faults. The second was a fault tolerant attitude control system for a micro satellite where the operation of the system is mission critical. The purpose was to avoid hazardous effects from faults and maintain operation if possible. A method...

  5. Regulatory T Cells Are Dispensable for Tolerance to RBC Antigens.

    Science.gov (United States)

    Richards, Amanda L; Kapp, Linda M; Wang, Xiaohong; Howie, Heather L; Hudson, Krystalyn E

    2016-01-01

    Autoimmune hemolytic anemia (AIHA) occurs when pathogenic autoantibodies against red blood cell (RBC) antigens are generated. While the basic disease pathology of AIHA is well studied, the underlying mechanism(s) behind the failure in tolerance to RBC autoantigens are poorly understood. Thus, to investigate the tolerance mechanisms required for the establishment and maintenance of tolerance to RBC antigens, we developed a novel murine model. With this model, we evaluated the role of regulatory T cells (Tregs) in tolerance to RBC-specific antigens. Herein, we show that neither sustained depletion of Tregs nor immunization with RBC-specific proteins in conjunction with Treg depletion led to RBC-specific autoantibody generation. Thus, these studies demonstrate that Tregs are not required to prevent autoantibodies to RBCs and suggest that other tolerance mechanisms are likely involved.

  6. Regulatory T cells are Dispensable for Tolerance to RBC Antigens

    Directory of Open Access Journals (Sweden)

    Amanda L Richards

    2016-09-01

    Full Text Available Autoimmune hemolytic anemia (AIHA occurs when pathogenic autoantibodies against red blood cell (RBC antigens are generated. Whilst the basic disease pathology of AIHA is well studied, the underlying mechanism(s behind the failure in tolerance to RBC autoantigens are poorly understood. Thus, to investigate the tolerance mechanisms required for the establishment and maintenance of tolerance to RBC antigens, we developed a novel murine model. With this model, we evaluated the role of regulatory T cells (Tregs in tolerance to RBC-specific antigens. Herein, we show that neither sustained depletion of Tregs nor immunization with RBC-specific proteins in conjunction with Treg depletion led to RBC-specific autoantibody generation. Thus, these studies demonstrate that Tregs are not required to prevent autoantibodies to RBCs and suggest that other tolerance mechanisms are likely involved.

  7. Transplantation tolerance mediated by regulatory T cells in mice

    Institute of Scientific and Technical Information of China (English)

    冯宁翰; 吴宏飞; 吴军; 张炜; 眭元庚; 贺厚光; 张春雷; 郑峻松

    2004-01-01

    Background With potent suppressive effect on responder T cells, CD4+CD25+ regulatory T (Treg) cells have become the focus of attention only recently and they may play an important role in transplantation tolerance. However, the mechanism of action is not clear. This study was designed to assess the possibility of using CD4+CD25+ Treg cells to induce transplantation tolerance and to investigate their mechanism of action.Methods CD4+CD25+ Treg cells were isolated using magnetic cell separation techniques. Mixed lymphocyte reactions were used to assess the ability of Treg cells to suppress effector T cells. Before skin transplantation, various numbers of CD4+CD25+Treg cells, which have been induced using complex skin antigens from the donor, were injected into the host mice either intraperitoneally (0.5×105, 1×105, 2×105, 3×105, 4×105, or 5×105) or by injection through the tail vein (5×103, 1×104, 2×104, 5×104, 1×105, 2×105). Skin grafts from two different donor types were used to assess whether the induced Treg cells were antigen-specific. The survival time of the allografts were observed. Single photon emission computed tomography was also used to determine the distribution of Treg cells before and after transplantation.Results Treg cells have suppressive effect on mixed lymphocyte reactions. Grafts survived longer in mice receiving CD4+CD25+ Treg cell injections than in control mice. There was a significant difference between groups receiving intraperitoneal injection of either 2×105 or 3×105 CD4+CD25+Treg cells and the control group (P<0.05, respectively). Better results were achieved when Treg cells were injected via the tail vein than when injected intraperitoneally. The transplantation tolerance induced by CD4+CD25+ Treg cells was donor-specific. Analysis of the localization of Treg cells revealed that Treg cells mainly migrated from the liver to the allografts and the spleen.Conclusions CD4+CD25+Treg cells can induce donor

  8. Fault Tolerant Wind Farm Control

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Stoustrup, Jakob

    2013-01-01

    with best at a wind turbine control level. However, some faults are better dealt with at the wind farm control level, if the wind turbine is located in a wind farm. In this paper a benchmark model for fault detection and isolation, and fault tolerant control of wind turbines implemented at the wind farm...... control level is presented. The benchmark model includes a small wind farm of nine wind turbines, based on simple models of the wind turbines as well as the wind and interactions between wind turbines in the wind farm. The model includes wind and power references scenarios as well as three relevant fault...... scenarios. This benchmark model is used in an international competition dealing with Wind Farm fault detection and isolation and fault tolerant control....

  9. Antigen-specific regulatory T-cell subsets in transplantation tolerance regulatory T-cell subset quality reduces the need for quantity.

    NARCIS (Netherlands)

    Koenen, H.J.P.M.; Joosten, I.

    2006-01-01

    Regulatory T cells (Treg) are critical controllers of the immune response. Disturbed Treg function results in autoimmunity, whereas in transplantation Treg are crucial in graft survival and transplant tolerance. Hence therapeutic modalities that influence Treg numbers or function hold great clinical

  10. Regulatory mechanisms of immune tolerance in type 1 diabetes and their failures.

    Science.gov (United States)

    Kuhn, Chantal; Besançon, Alix; Lemoine, Sébastien; You, Sylvaine; Marquet, Cindy; Candon, Sophie; Chatenoud, Lucienne

    2016-07-01

    In this brief review we propose to discuss salient data showing the importance of immune regulatory mechanisms, and in particular of Treg, for the control of pathogenic anti-β-cell response in autoimmune diabetes. Disease progression that culminates with the massive destruction of insulin-secreting β-cells and advent of hyperglycemia and glycosuria tightly correlates with a functional deficit in immune regulation. Better dissection of the cellular and molecular mechanisms through which the immune system normally sustains tolerance to "self", and which become defective when autoimmune aggression is overt, is the only direct and robust way to learn how to harness these effectively, so as to restore immune tolerance in patients with insulin-dependent type 1 diabetes. No doubt that regulatory T cells are a privileged mechanism underlying this self-tolerance in the periphery. The discovery of the key role of the transcription factor FoxP3, represented the cornerstone leading to the great advances in the field we are witnessing today. Type 1 diabetes is certainly one of the prototypic T cell-mediated autoimmune diseases where immune regulatory mechanisms relying on specialized subsets of T cells have been the most thoroughly analyzed from the fundamental point of view and also largely exploited in a translational therapeutic perspective.

  11. Regulatory T Cell Specificity Directs Tolerance versus Allergy against Aeroantigens in Humans.

    Science.gov (United States)

    Bacher, Petra; Heinrich, Frederik; Stervbo, Ulrik; Nienen, Mikalai; Vahldieck, Marco; Iwert, Christina; Vogt, Katrin; Kollet, Jutta; Babel, Nina; Sawitzki, Birgit; Schwarz, Carsten; Bereswill, Stefan; Heimesaat, Markus M; Heine, Guido; Gadermaier, Gabriele; Asam, Claudia; Assenmacher, Mario; Kniemeyer, Olaf; Brakhage, Axel A; Ferreira, Fátima; Wallner, Michael; Worm, Margitta; Scheffold, Alexander

    2016-11-03

    FOXP3+ regulatory T cells (Tregs) maintain tolerance against self-antigens and innocuous environmental antigens. However, it is still unknown whether Treg-mediated tolerance is antigen specific and how Treg specificity contributes to the selective loss of tolerance, as observed in human immunopathologies such as allergies. Here, we used antigen-reactive T cell enrichment to identify antigen-specific human Tregs. We demonstrate dominant Treg-mediated tolerance against particulate aeroallergens, such as pollen, house dust mites, and fungal spores. Surprisingly, we found no evidence of functional impairment of Treg responses in allergic donors. Rather, major allergenic proteins, known to rapidly dissociate from inhaled allergenic particles, have a generally reduced capability to generate Treg responses. Most strikingly, in individual allergic donors, Th2 cells and Tregs always target disparate proteins. Thus, our data highlight the importance of Treg antigen-specificity for tolerance in humans and identify antigen-specific escape from Treg control as an important mechanism enabling antigen-specific loss of tolerance in human allergy. Copyright © 2016 Elsevier Inc. All rights reserved.

  12. Immune regulatory effects of simvastatin on regulatory T cell-mediated tumour immune tolerance.

    Science.gov (United States)

    Lee, K J; Moon, J Y; Choi, H K; Kim, H O; Hur, G Y; Jung, K H; Lee, S Y; Kim, J H; Shin, C; Shim, J J; In, K H; Yoo, S H; Kang, K H; Lee, S Y

    2010-08-01

    Statins are potent inhibitors of hydroxyl-3-methylglutaryl co-enzyme A (HMG-CoA) reductase, and have emerged as potential anti-cancer agents based on preclinical evidence. In particular, compelling evidence suggests that statins have a wide range of immunomodulatory properties. However, little is known about the role of statins in tumour immune tolerance. Tumour immune tolerance involves the production of immunosuppressive molecules, such as interleukin (IL)-10, transforming growth factor (TGF)-beta and indoleamine-2,3-dioxygenase (IDO) by tumours, which induce a regulatory T cell (T(reg)) response. In this study, we investigated the effect of simvastatin on the production of IL-10, TGF-beta and IDO production and the proliferation of T(regs) using several cancer cell lines, and Lewis lung cancer (3LL) cells-inoculated mouse tumour model. Simvastatin treatment resulted in a decrease in the number of cancer cells (3LL, A549 and NCI-H292). The production of the immune regulatory markers IL-10, TGF-beta in 3LL and NCI-H292 cells increased after treatment with simvastatin. The expression of IDO and forkhead box P3 (FoxP3) transcription factor was also increased in the presence of simvastatin. In a murine 3LL model, there were no significant differences in tumour growth rate between untreated and simvastatin-treated mice groups. Therefore, while simvastatin had an anti-proliferative effect, it also exhibited immune tolerance-promoting properties during tumour development. Thus, due to these opposing actions, simvastatin had no net effect on tumour growth.

  13. Oral tolerance to cancer can be abrogated by T regulatory cell inhibition.

    Directory of Open Access Journals (Sweden)

    Maria C Whelan

    Full Text Available Oral administration of tumour cells induces an immune hypo-responsiveness known as oral tolerance. We have previously shown that oral tolerance to a cancer is tumour antigen specific, non-cross-reactive and confers a tumour growth advantage. We investigated the utilisation of regulatory T cell (Treg depletion on oral tolerance to a cancer and its ability to control tumour growth. Balb/C mice were gavage fed homogenised tumour tissue--JBS fibrosarcoma (to induce oral tolerance to a cancer, or PBS as control. Growth of subcutaneous JBS tumours were measured; splenic tissue excised and flow cytometry used to quantify and compare systemic Tregs and T effector (Teff cell populations. Prior to and/or following tumour feeding, mice were intraperitoneally administered anti-CD25, to inactivate systemic Tregs, or given isotype antibody as a control. Mice which were orally tolerised prior to subcutaneous tumour induction, displayed significantly higher systemic Treg levels (14% vs 6% and faster tumour growth rates than controls (p<0.05. Complete regression of tumours were only seen after Treg inactivation and occurred in all groups--this was not inhibited by tumour feeding. The cure rates for Treg inactivation were 60% during tolerisation, 75% during tumour growth and 100% during inactivation for both tolerisation and tumour growth. Depletion of Tregs gave rise to an increased number of Teff cells. Treg depletion post-tolerisation and post-tumour induction led to the complete regression of all tumours on tumour bearing mice. Oral administration of tumour tissue, confers a tumour growth advantage and is accompanied by an increase in systemic Treg levels. The administration of anti-CD25 Ab decreased Treg numbers and caused an increase in Teffs. Most notably Treg cell inhibition overcame established oral tolerance with consequent tumor regression, especially relevant to foregut cancers where oral tolerance is likely to be induced by the shedding of tumour

  14. Radiation tolerant power converter controls

    Science.gov (United States)

    Todd, B.; Dinius, A.; King, Q.; Uznanski, S.

    2012-11-01

    The Large Hadron Collider (LHC) at the European Organisation for Nuclear Research (CERN) is the world's most powerful particle collider. The LHC has several thousand magnets, both warm and super-conducting, which are supplied with current by power converters. Each converter is controlled by a purpose-built electronic module called a Function Generator Controller (FGC). The FGC allows remote control of the power converter and forms the central part of a closed-loop control system where the power converter voltage is set, based on the converter output current and magnet-circuit characteristics. Some power converters and FGCs are located in areas which are exposed to beam-induced radiation. There are numerous radiation induced effects, some of which lead to a loss of control of the power converter, having a direct impact upon the accelerator's availability. Following the first long shut down (LS1), the LHC will be able to run with higher intensity beams and higher beam energy. This is expected to lead to significantly increased radiation induced effects in materials close to the accelerator, including the FGC. Recent radiation tests indicate that the current FGC would not be sufficiently reliable. A so-called FGClite is being designed to work reliably in the radiation environment in the post-LS1 era. This paper outlines the concepts of power converter controls for machines such as the LHC, introduces the risks related to radiation and a radiation tolerant project flow. The FGClite is then described, with its key concepts and challenges: aiming for high reliability in a radiation field.

  15. Diagnosis and Fault-tolerant Control

    DEFF Research Database (Denmark)

    Blanke, Mogens; Kinnaert, Michel; Lunze, Jan

    The book presents effective model-based analysis and design methods for fault diagnosis and fault-tolerant control. Architectural and structural models are used to analyse the propagation of the fault through the process, to test the fault detectability and to find the redundancies in the process...... that can be used to ensure fault tolerance. Design methods for diagnostic systems and fault-tolerant controllers are presented for processes that are described by analytical models, by discrete-event models or that can be dealt with as quantised systems. Four case studies on pilot processes show......-tolerant control....

  16. Fault tolerant control design for hybrid systems

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Hao; Jiang, Bin [Nanjing University of Aeronautics and Astronautics, Nanjing (China); Cocquempot, Vincent [Universite des Sciences et Technologies de Lille, Villeneuve d' Ascq (France)

    2010-07-01

    This book intends to provide the readers a good understanding on how to achieve Fault Tolerant Control goal of Hybrid Systems. The book can be used as a reference for the academic research on Fault Tolerant Control and Hybrid Systems or used in Ph.D. study of control theory and engineering. The knowledge background for this monograph would be some undergraduate and graduate courses on Fault Diagnosis and Fault Tolerant Control theory, linear system theory, nonlinear system theory, Hybrid Systems theory and Discrete Event System theory. (orig.)

  17. Hyperlipidemia Alters Regulatory T Cell Function and Promotes Resistance to Tolerance Induction Through Costimulatory Molecule Blockade.

    Science.gov (United States)

    Bagley, J; Yuan, J; Chandrakar, A; Iacomini, J

    2015-09-01

    Recent work from our laboratory has shown that hyperlipidemia promotes accelerated rejection of vascularized cardiac allografts in mice by inducing anti-donor Th17 reactivity and production of IL-17. Here, we show that hyperlipidemia also affects FoxP3(+) regulatory T cells (Tregs). Hyperlipidemia promotes the development of Tregs that express low levels of CD25. Hyperlipidemia also promotes a decrease in central Tregs and an increase in effector Tregs that appears to account for the increase in the frequency of CD25(low) Tregs. Alterations in Treg subsets also appear to lead to alterations in Treg function. The ability of FoxP3(+) , CD25(high) , CD4(+) Tregs from hyperlipidemic mice to inhibit proliferation of effector T cells stimulated with anti-CD3 and CD28 was reduced when compared with Tregs from control mice. Regulatory T cells isolated from hyperlipidemic recipients exhibit increased activation of Akt, and a reduction in Bim levels that permits the expansion of FoxP3(+) CD25(low) CD4(+) T cells. Hyperlipidemic mice were also resistant to tolerance induction using costimulatory molecule blockade consisting of anti-CD154 and CTLA4Ig, a strategy that requires Tregs. Together, our data suggest that hyperlipidemia profoundly affects Treg subsets and function as well as the ability to induce tolerance.

  18. Diagnosis and fault-tolerant control

    CERN Document Server

    Blanke, Mogens; Lunze, Jan; Staroswiecki, Marcel

    2016-01-01

    Fault-tolerant control aims at a gradual shutdown response in automated systems when faults occur. It satisfies the industrial demand for enhanced availability and safety, in contrast to traditional reactions to faults, which bring about sudden shutdowns and loss of availability. The book presents effective model-based analysis and design methods for fault diagnosis and fault-tolerant control. Architectural and structural models are used to analyse the propagation of the fault through the process, to test the fault detectability and to find the redundancies in the process that can be used to ensure fault tolerance. It also introduces design methods suitable for diagnostic systems and fault-tolerant controllers for continuous processes that are described by analytical models of discrete-event systems represented by automata. The book is suitable for engineering students, engineers in industry and researchers who wish to get an overview of the variety of approaches to process diagnosis and fault-tolerant contro...

  19. Fault tolerant control for switched linear systems

    CERN Document Server

    Du, Dongsheng; Shi, Peng

    2015-01-01

    This book presents up-to-date research and novel methodologies on fault diagnosis and fault tolerant control for switched linear systems. It provides a unified yet neat framework of filtering, fault detection, fault diagnosis and fault tolerant control of switched systems. It can therefore serve as a useful textbook for senior and/or graduate students who are interested in knowing the state-of-the-art of filtering, fault detection, fault diagnosis and fault tolerant control areas, as well as recent advances in switched linear systems.  

  20. Identification and reconstitution of genetic regulatory networks for improved microbial tolerance to isooctane.

    Science.gov (United States)

    Kang, Aram; Chang, Matthew Wook

    2012-04-01

    Microbial tolerance to hydrocarbons has been studied in an effort to improve the productivity of biochemical processes and to enhance the efficiency of hydrocarbon bioremediation. Despite these studies, few attempts have been made to design rational strategies to improve microbial tolerance to hydrocarbons. Herein, we present an engineering framework that enables us to harness our understanding of genetic regulatory networks to improve hydrocarbon tolerance. In this study, isooctane was used as a representative hydrocarbon due to its use in petroleum refining and in biochemical processes. To increase isooctane tolerance, we first identified essential transcriptional determinants and genetic regulatory networks underlying cellular responses to isooctane in Escherichia coli using genome-wide microarray analysis. Based on functional transcriptome and bioinformatics analysis, a range of combinations of transcription factors whose activity was predictably perturbed by isooctane were knocked out and overexpressed to reconstitute the regulatory networks. We demonstrated that the reconstitution of the regulatory networks led to a significant improvement in isooctane tolerance, and especially, engineered E. coli strains lacking and overexpressing some of the perturbed transcription factors showed 3- to 5-fold improvement. This microbe with high tolerance to isooctane can be harnessed for biochemical processes, fuel oil bioremediation and metabolic engineering for biofuel production. Furthermore, we envision that the engineering framework employed to improve the tolerance in this study can be exploited for developing other microbes with desired phenotypes.

  1. FoxP3+ regulatory T cells essentially contribute to peripheral CD8+ T-cell tolerance induced by steady-state dendritic cells

    Science.gov (United States)

    Schildknecht, Anita; Brauer, Sabine; Brenner, Corinne; Lahl, Katharina; Schild, Hansjörg; Sparwasser, Tim; Probst, Hans Christian; van den Broek, Maries

    2010-01-01

    Peripheral T-cell tolerance is thought to significantly contribute to the prevention of autoimmunity, and it has been shown that antigen-presenting steady-state dendritic cells efficiently induce peripheral tolerance. We previously showed that dendritic-cell–induced tolerance is a T-cell–intrinsic process that depends on coinhibitory molecules such as programmed death-1. Here we specifically analyze the involvement of FoxP3+ regulatory T cells, which are known to be important for maintenance of self-tolerance. We show that antigen presentation by steady-state dendritic cells failed to induce peripheral tolerance in the absence of FoxP3+ regulatory T cells but induced protective CD8+ T-cell–mediated immunity instead. Regulatory T-cell–depleted mice had massively increased numbers of dendritic cells in lymph nodes. Dendritic cells isolated from mice without regulatory T cells had up-regulated costimulatory molecules and showed stronger T-cell stimulatory capacity ex vivo, suggesting that regulatory T cells contribute to peripheral tolerance by keeping the dendritic cells in an immature state. Using blocking antibodies, we demonstrate that CTLA-4 but not IL-10 is necessary for control of dendritic cells by regulatory T cells. PMID:20018763

  2. Control switching in high performance and fault tolerant control

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik; Poulsen, Niels Kjølstad

    2010-01-01

    The problem of reliability in high performance control and in fault tolerant control is considered in this paper. A feedback controller architecture for high performance and fault tolerance is considered. The architecture is based on the Youla-Jabr-Bongiorno-Kucera (YJBK) parameterization. By usi...

  3. Furfural and hydroxymethylfurfural tolerance in Escherichia coli ΔacrR regulatory mutants.

    Science.gov (United States)

    Luhe, Annette Lin; Lim, Chan Yuen; Gerken, Henri; Wu, Jinchuan; Zhao, Hua

    2015-01-01

    The presence of the highly toxic furfural and hydroxymethylfurfural (HMF) in the hydrolysate of lignocellulosic biomass prompted the investigation of the Escherichia coli ΔacrR regulatory mutant for higher tolerance to these compounds, to facilitate the production of biofuels and biochemicals, and further biocatalytic conversions. In comparison with the parental strain, the regulatory mutant with the upregulated efflux pump AcrAB-TolC produced moderately better growth and higher tolerance to concentrations of furfural and HMF between 1 and 2 g L(-1) .

  4. Clinical perspectives for regulatory T cells in transplantation tolerance

    OpenAIRE

    Hippen, Keli L.; Riley, James L.; June, Carl H.; Blazar, Bruce R.

    2011-01-01

    Three main types of CD4+ regulatory T cells can be distinguished based upon whether they express Foxp3 and differentiate naturally in the thymus (natural Tregs) or are induced in the periphery (inducible Tregs); or whether they are FoxP3 negative but secrete IL-10 in response to antigen (Tregulatory type 1, Tr1 cells). Adoptive transfer of each cell type has proven highly effective in mouse models at preventing graft vs. host disease (GVHD) and autoimmunity. Although clinical application was ...

  5. An architecture for fault tolerant controllers

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik; Stoustrup, Jakob

    2005-01-01

    degradation in the sense of guaranteed degraded performance. A number of fault diagnosis problems, fault tolerant control problems, and feedback control with fault rejection problems are formulated/considered, mainly from a fault modeling point of view. The method is illustrated on a servo example including......A general architecture for fault tolerant control is proposed. The architecture is based on the (primary) YJBK parameterization of all stabilizing compensators and uses the dual YJBK parameterization to quantify the performance of the fault tolerant system. The approach suggested can be applied...... for additive faults, parametric faults, and for system structural changes. The modeling for each of these fault classes is described. The method allows to design for passive as well as for active fault handling. Also, the related design method can be fitted either to guarantee stability or to achieve graceful...

  6. Radiation tolerant power converter controls

    CERN Document Server

    Todd, B; King, Q; Uznanski, S

    2012-01-01

    The Large Hadron Collider (LHC) at the European Organisation for Nuclear Research (CERN) is the world's most powerful particle collider. The LHC has several thousand magnets, both warm and super-conducting, which are supplied with current by power converters. Each converter is controlled by a purpose-built electronic module called a Function Generator Controller (FGC). The FGC allows remote control of the power converter and forms the central part of a closed-loop control system where the power converter voltage is set, based on the converter output current and magnet-circuit characteristics. Some power converters and FGCs are located in areas which are exposed to beam-induced radiation. There are numerous radiation induced effects, some of which lead to a loss of control of the power converter, having a direct impact upon the accelerator's availability. Following the first long shut down (LS1), the LHC will be able to run with higher intensity beams and higher beam energy. This is expected to lead to signifi...

  7. Regulatory T-cells and immune tolerance in pregnancy : a new target for infertility treatment?

    NARCIS (Netherlands)

    Guerin, Leigh R.; Prins, Jelmer R.; Robertson, Sarah A.

    2009-01-01

    Adaptation of the maternal immune response to accommodate the semi-allogeneic fetus is necessary for pregnancy success, and disturbances in maternal tolerance are implicated in infertility and reproductive pathologies. T regulatory (Treg) cells are a recently discovered subset of T-lymphocytes with

  8. Understanding regulatory networks and engineering for enhanced drought tolerance in plants.

    Science.gov (United States)

    Valliyodan, Babu; Nguyen, Henry T

    2006-04-01

    Drought stress is one of the major limitations to crop productivity. To develop crop plants with enhanced tolerance of drought stress, a basic understanding of physiological, biochemical and gene regulatory networks is essential. Various functional genomics tools have helped to advance our understanding of stress signal perception and transduction, and of the associated molecular regulatory network. These tools have revealed several stress-inducible genes and various transcription factors that regulate the drought-stress-inducible systems. Translational genomics of these candidate genes using model plants provided encouraging results, but the field testing of transgenic crop plants for better performance and yield is still minimal. Better understanding of the specific roles of various metabolites in crop stress tolerance will give rise to a strategy for the metabolic engineering of crop tolerance of drought.

  9. Fault-tolerant Supervisory Control

    DEFF Research Database (Denmark)

    Izadi-Zamanabadi, Roozbeh

    of this work has been to develop and employ concepts and methods that are suitable for use in different automation processes, with applicability in various industrial fields. The requirements for high productivity and quality has resulted in employing additional instrumentation and use of more sophisticated...... could be increased through enhancing control systems' ability to on-line perform fault detection and reconfiguration when a fault occurs and before a safety system shuts-down the entire process. The main contributions of this research effort are development and experimentation with methodologies......, is extended to cope with the important reconfiguration problem. In order to enable a designer to acquire knowledge about reconfiguration possibilities, the structural analysis method is added as an extension to the existing methodology. This extension builds upon the earlier method where fault propagation...

  10. The Induction and Maintenance of Transplant Tolerance Engages Both Regulatory and Anergic CD4+ T cells

    Science.gov (United States)

    Besançon, Alix; Baas, Marije; Goncalves, Tania; Valette, Fabrice; Waldmann, Herman; Chatenoud, Lucienne; You, Sylvaine

    2017-01-01

    Therapeutic tolerance to self-antigens or foreign antigens is thought to depend on constant vigilance by Foxp3+ regulatory T cells (Tregs). Previous work using a pancreatic islet allograft model and a short pulse of CD3 antibody therapy has shown that CD8+ T cells become anergic and use TGFβ and coinhibitory signaling as their contribution to the tolerance process. Here, we examine the role of CD4+ T cells in tolerization by CD3 antibodies. We show that both Foxp3+ Tregs and CD4+ T cell anergy play a role in the induction of tolerance and its maintenance. Foxp3+ Tregs resisted CD3 antibody-mediated depletion, unlike intragraft Th1 CD4+ lymphocytes coexpressing granzyme B and Tbx21, which were selectively eliminated. Tregs were mandatory for induction of tolerance as their depletion at the time of CD3 antibody therapy or for a short time thereafter, by an antibody to CD25 (PC61), led to graft rejection. Early treatment with CTLA-4 antibody gave the same outcome. In contrast, neither PC61 nor anti-CTLA-4 given late, at day 100 posttransplant, reversed tolerance once established. Ablation of Foxp3 T cells after diphtheria toxin injection in tolerant Foxp3DTR recipient mice provided the same outcome. Alloreactive T cells had been rendered intrinsically unresponsive as total CD4+ or Treg-deprived CD4+ T cells from tolerant recipients were unable to mount donor-specific IFN-γ responses. In addition, intragraft Treg-deprived CD4+ T cells lacked proliferative capacities, expressed high levels of the inhibitory receptor PD-1, and exhibited a CD73hiFR4hi phenotype, thus reflecting a state of T cell anergy. We conclude that Tregs play a substantive and critical role in guiding the immune system toward tolerance of the allograft, when induced by CD3 antibody, but are less important for maintenance of the tolerant state, where T cell anergy appears sufficient.

  11. The Induction and Maintenance of Transplant Tolerance Engages Both Regulatory and Anergic CD4(+) T cells.

    Science.gov (United States)

    Besançon, Alix; Baas, Marije; Goncalves, Tania; Valette, Fabrice; Waldmann, Herman; Chatenoud, Lucienne; You, Sylvaine

    2017-01-01

    Therapeutic tolerance to self-antigens or foreign antigens is thought to depend on constant vigilance by Foxp3(+) regulatory T cells (Tregs). Previous work using a pancreatic islet allograft model and a short pulse of CD3 antibody therapy has shown that CD8(+) T cells become anergic and use TGFβ and coinhibitory signaling as their contribution to the tolerance process. Here, we examine the role of CD4(+) T cells in tolerization by CD3 antibodies. We show that both Foxp3(+) Tregs and CD4(+) T cell anergy play a role in the induction of tolerance and its maintenance. Foxp3(+) Tregs resisted CD3 antibody-mediated depletion, unlike intragraft Th1 CD4(+) lymphocytes coexpressing granzyme B and Tbx21, which were selectively eliminated. Tregs were mandatory for induction of tolerance as their depletion at the time of CD3 antibody therapy or for a short time thereafter, by an antibody to CD25 (PC61), led to graft rejection. Early treatment with CTLA-4 antibody gave the same outcome. In contrast, neither PC61 nor anti-CTLA-4 given late, at day 100 posttransplant, reversed tolerance once established. Ablation of Foxp3 T cells after diphtheria toxin injection in tolerant Foxp3(DTR) recipient mice provided the same outcome. Alloreactive T cells had been rendered intrinsically unresponsive as total CD4(+) or Treg-deprived CD4(+) T cells from tolerant recipients were unable to mount donor-specific IFN-γ responses. In addition, intragraft Treg-deprived CD4(+) T cells lacked proliferative capacities, expressed high levels of the inhibitory receptor PD-1, and exhibited a CD73(hi)FR4(hi) phenotype, thus reflecting a state of T cell anergy. We conclude that Tregs play a substantive and critical role in guiding the immune system toward tolerance of the allograft, when induced by CD3 antibody, but are less important for maintenance of the tolerant state, where T cell anergy appears sufficient.

  12. Regulatory B cells and tolerance in transplantation: from animal models to human.

    Directory of Open Access Journals (Sweden)

    Melanie eChesneau

    2013-12-01

    Full Text Available Until recently, the role of B cells in transplantation was thought to be restricted to producing antibodies that have been clearly shown to be deleterious in the long term, but, in fact, B cells are also able to produce cytokine and to present antigen. Their role as regulatory cells in various pathological situations has also been highlighted, and their role in transplantation is beginning to emerge in animal, and also in human, models. This review summarizes the different studies in animals and humans that suggest a B-cell regulatory role in the transplant tolerance mechanisms.

  13. Fault Tolerant Control Using Gaussian Processes and Model Predictive Control

    Directory of Open Access Journals (Sweden)

    Yang Xiaoke

    2015-03-01

    Full Text Available Essential ingredients for fault-tolerant control are the ability to represent system behaviour following the occurrence of a fault, and the ability to exploit this representation for deciding control actions. Gaussian processes seem to be very promising candidates for the first of these, and model predictive control has a proven capability for the second. We therefore propose to use the two together to obtain fault-tolerant control functionality. Our proposal is illustrated by several reasonably realistic examples drawn from flight control.

  14. Mapping regulatory genes as candidates for cold and drought stress tolerance in barley.

    Science.gov (United States)

    Tondelli, A; Francia, E; Barabaschi, D; Aprile, A; Skinner, J S; Stockinger, E J; Stanca, A M; Pecchioni, N

    2006-02-01

    Cereal crop yield is greatly affected in many growing areas by abiotic stresses, mainly low temperature and drought. In order to find candidates for the tolerance genes for these stresses, 13 genes encoding for transcription factors and upstream regulators were screened by amplification and SSCP on six parental genotypes of three barley mapping populations ('Nure' x 'Tremois', 'Proctor' x 'Nudinka', and 'Steptoe' x 'Morex'), and mapped as newly developed STS, SNP, and SSCP markers. A new consensus function map was then drawn using the three maps above, including 16 regulatory candidate genes (CGs). The positions of barley cold and drought tolerance quantitative trait loci (QTLs) presently described in the literature were added to the consensus map to find positional candidates from among the mapped genes. A cluster of six HvCBF genes co-mapped with the Fr-H2 cold tolerance QTL, while no QTLs for the same trait were positioned on chromosome 7H, where two putative barley regulators of CBF expression, ICE1 and FRY1, found by homology search, were mapped in this work. These observations suggest that CBF gene(s) themselves, rather than their two regulators, are at present the best candidates for cold tolerance. Four out of 12 drought tolerance QTLs of the consensus map are associated with regulatory CGs, on chromosomes 2H, 5H, and 7H, and two QTLs with effector genes, on chromosomes 5H and 6H. The results obtained could be used to guide MAS applications, allowing introduction into an ideal genotype of favourable alleles of tolerance QTLs.

  15. Mucosally induced immunological tolerance, regulatory T cells and the adjuvant effect by cholera toxin B subunit.

    Science.gov (United States)

    Sun, J-B; Czerkinsky, C; Holmgren, J

    2010-01-01

    Induction of peripheral immunological tolerance by mucosal administration of selected antigens (Ags) ('oral tolerance') is an attractive, yet medically little developed, approach to prevent or treat selected autoimmune or allergic disorders. A highly effective way to maximize oral tolerance induction for immunotherapeutic purposes is to administer the relevant Ag together with, and preferably linked to the non-toxic B subunit protein of cholera toxin (CTB). Oral, nasal or sublingual administration of such Ag/CTB conjugates or gene fusion proteins have been found to induce tolerance with superior efficiency compared with administration of Ag alone, including the suppression of various autoimmune disorders and allergies in animal models. In a proof-of-concept clinical trial in patients with Behcet's disease, this was extended with highly promising results to prevent relapse of autoimmune uveitis. Tolerization by mucosal Ag/CTB administration results in a strong increase in Ag-specific regulatory CD4(+) T cells, apparently via two separate pathways: one using B cells as APCs and leading to a strong expansion of Foxp3(+) Treg cells which can both suppress and mediate apoptotic depletion of effector T cells, and one being B cell-independent and associated with development of Foxp3(-) regulatory T cells that express membrane latency-associated peptide and transforming growth factor (TGF-beta) and/or IL-10. The ability of CTB to dramatically increase mucosal Ag uptake and presentation by different APCs through binding to GM1 ganglioside (which makes most B cells effective APCs irrespective of their Ag specificity), together with CTB-mediated stimulation of TGF-beta and IL-10 production and inhibition of IL-6 formation may explain the dramatic potentiation of oral tolerance by mucosal Ags presented with CTB.

  16. Diagnosis and Fault-tolerant Control

    DEFF Research Database (Denmark)

    Blanke, Mogens; Kinnaert, Michel; Lunze, Jan;

    The book presents effective model-based analysis and design methods for fault diagnosis and fault-tolerant control. Architectural and structural models are used to analyse the propagation of the fault through the process, to test the fault detectability and to find the redundancies in the process...... the applicability of the presented methods. The theoretical results are illustrated by two running examples which are used throughout the book. The book addresses engineering students, engineers in industry and researchers who wish to get a survey over the variety of approaches to process diagnosis and fault...

  17. Lung-resident tissue macrophages generate Foxp3+ regulatory T cells and promote airway tolerance.

    Science.gov (United States)

    Soroosh, Pejman; Doherty, Taylor A; Duan, Wei; Mehta, Amit Kumar; Choi, Heonsik; Adams, Yan Fei; Mikulski, Zbigniew; Khorram, Naseem; Rosenthal, Peter; Broide, David H; Croft, Michael

    2013-04-01

    Airway tolerance is the usual outcome of inhalation of harmless antigens. Although T cell deletion and anergy are likely components of tolerogenic mechanisms in the lung, increasing evidence indicates that antigen-specific regulatory T cells (inducible Treg cells [iTreg cells]) that express Foxp3 are also critical. Several lung antigen-presenting cells have been suggested to contribute to tolerance, including alveolar macrophages (MØs), classical dendritic cells (DCs), and plasmacytoid DCs, but whether these possess the attributes required to directly promote the development of Foxp3(+) iTreg cells is unclear. Here, we show that lung-resident tissue MØs coexpress TGF-β and retinal dehydrogenases (RALDH1 and RALDH 2) under steady-state conditions and that their sampling of harmless airborne antigen and presentation to antigen-specific CD4 T cells resulted in the generation of Foxp3(+) Treg cells. Treg cell induction in this model depended on both TGF-β and retinoic acid. Transfer of the antigen-pulsed tissue MØs into the airways correspondingly prevented the development of asthmatic lung inflammation upon subsequent challenge with antigen. Moreover, exposure of lung tissue MØs to allergens suppressed their ability to generate iTreg cells coincident with blocking airway tolerance. Suppression of Treg cell generation required proteases and TLR-mediated signals. Therefore, lung-resident tissue MØs have regulatory functions, and strategies to target these cells might hold promise for prevention or treatment of allergic asthma.

  18. Regulatory roles of serotonin and melatonin in abiotic stress tolerance in plants.

    Science.gov (United States)

    Kaur, Harmeet; Mukherjee, Soumya; Baluska, Frantisek; Bhatla, Satish C

    2015-01-01

    Understanding the physiological and biochemical basis of abiotic stress tolerance in plants has always been one of the major aspects of research aiming to enhance plant productivity in arid and semi-arid cultivated lands all over the world. Growth of stress-tolerant transgenic crops and associated agricultural benefits through increased productivity, and related ethical issues, are also the major concerns of current research in various laboratories. Interesting data on the regulation of abiotic stress tolerance in plants by serotonin and melatonin has accumulated in the recent past. These two indoleamines possess antioxidative and growth-inducing properties, thus proving beneficial for stress acclimatization. Present review shall focus on the modes of serotonin and melatonin-induced regulation of abiotic stress tolerance in plants. Complex molecular interactions of serotonin and auxin-responsive genes have suggested their antagonistic nature. Data from genomic and metabolomic analyses of melatonin-induced abiotic stress signaling have lead to an understanding of the regulation of stress tolerance through the modulation of transcription factors, enzymes and various signaling molecules. Melatonin, nitric oxide (NO) and calmodulin interactions have provided new avenues for research on the molecular aspects of stress physiology in plants. Investigations on the characterization of receptors associated with serotonin and melatonin responses, are yet to be undertaken in plants. Patenting of biotechnological inventions pertaining to serotonin and melatonin formulations (through soil application or foliar spray) are expected to be some of the possible ways to regulate abiotic stress tolerance in plants. The present review, thus, summarizes the regulatory roles of serotonin and melatonin in modulating the signaling events accompanying abiotic stress in plants.

  19. Do pharmacological approaches that prevent opioid tolerance target different elements in the same regulatory machinery?

    Science.gov (United States)

    Garzón, Javier; Rodríguez-Muñoz, María; Sánchez-Blázquez, Pilar

    2008-06-01

    In the nervous system, the interaction of opioids like heroin and morphine with the G protein-coupled Mu-opioid receptor (MOR) provokes the development of tolerance to these opioids, as well as physical dependence. Tolerance implies that higher doses of these drugs must be consumed in order to obtain an equivalent sensation, a situation that contributes notably to the social problems surrounding recreational opioid abuse. The mechanisms that promote opioid tolerance involve a series of adaptive changes in the MOR and in the post-receptor signalling elements. Pharmacological studies have consistently identified a number of signalling proteins relevant to morphine-induced tolerance, including the delta-opioid receptor (DOR), protein kinase C (PKC), protein kinase A (PKA), calcium/calmodulin-dependent kinase II (CaMKII), nitric oxide synthase (NOS), N-methyl-D-aspartate acid glutamate receptors (NMDAR), and regulators of G-signalling (RGS) proteins. Thus, it is feasible that these treatments which diminish morphine tolerance target distinct elements within the same regulatory machinery. In this scheme, the signals originated at the agonist-activated MORs would be recognised by elements such as the NMDARs, which in turn exert a negative feedback on MOR-evoked signalling. This process involves DOR regulation of MORs, MOR-induced activation of NMDARs (probably via the regulation of Src, recruiting PKC and Galpha subunits) and the NMDAR-mediated activation of CaMKII. The active CaMKII promotes the sequestering of morphine-activated Gbetagamma dimers by phosducin-like proteins (PhLP) and of Galpha subunits by RGS proteins and tolerance to opioids like morphine develops. Future efforts to study these phenomena should focus on fitting additional pieces into this puzzle in order to fully define the mechanism underlying the desensitization of MORs in neural cells.

  20. Aire Enforces Immune Tolerance by Directing Autoreactive T Cells into the Regulatory T Cell Lineage.

    Science.gov (United States)

    Malchow, Sven; Leventhal, Daniel S; Lee, Victoria; Nishi, Saki; Socci, Nicholas D; Savage, Peter A

    2016-05-17

    The promiscuous expression of tissue-restricted antigens in the thymus, driven in part by autoimmune regulator (Aire), is critical for the protection of peripheral tissues from autoimmune attack. Aire-dependent processes are thought to promote both clonal deletion and the development of Foxp3(+) regulatory T (Treg) cells, suggesting that autoimmunity associated with Aire deficiency results from two failed tolerance mechanisms. Here, examination of autoimmune lesions in Aire(-/-) mice revealed an unexpected third possibility. We found that the predominant conventional T cell clonotypes infiltrating target lesions express antigen receptors that were preferentially expressed by Foxp3(+) Treg cells in Aire(+/+) mice. Thus, Aire enforces immune tolerance by ensuring that distinct autoreactive T cell specificities differentiate into the Treg cell lineage; dysregulation of this process results in the diversion of Treg cell-biased clonotypes into pathogenic conventional T cells. Copyright © 2016 Elsevier Inc. All rights reserved.

  1. Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants.

    Science.gov (United States)

    Todaka, Daisuke; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko

    2015-01-01

    Advances have been made in the development of drought-tolerant transgenic plants, including cereals. Rice, one of the most important cereals, is considered to be a critical target for improving drought tolerance, as present-day rice cultivation requires large quantities of water and as drought-tolerant rice plants should be able to grow in small amounts of water. Numerous transgenic rice plants showing enhanced drought tolerance have been developed to date. Such genetically engineered plants have generally been developed using genes encoding proteins that control drought regulatory networks. These proteins include transcription factors, protein kinases, receptor-like kinases, enzymes related to osmoprotectant or plant hormone synthesis, and other regulatory or functional proteins. Of the drought-tolerant transgenic rice plants described in this review, approximately one-third show decreased plant height under non-stressed conditions or in response to abscisic acid treatment. In cereal crops, plant height is a very important agronomic trait directly affecting yield, although the improvement of lodging resistance should also be taken into consideration. Understanding the regulatory mechanisms of plant growth reduction under drought stress conditions holds promise for developing transgenic plants that produce high yields under drought stress conditions. Plant growth rates are reduced more rapidly than photosynthetic activity under drought conditions, implying that plants actively reduce growth in response to drought stress. In this review, we summarize studies on molecular regulatory networks involved in response to drought stress. In a separate section, we highlight progress in the development of transgenic drought-tolerant rice plants, with special attention paid to field trial investigations.

  2. Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants

    Directory of Open Access Journals (Sweden)

    Daisuke eTodaka

    2015-02-01

    Full Text Available Advances have been made in the development of drought-tolerant transgenic plants, including cereals. Rice, one of the most important cereals, is considered to be a critical target for improving drought tolerance, as present-day rice cultivation requires large quantities of water and as drought-tolerant rice plants should be able to grow in small amounts of water. Numerous transgenic rice plants showing enhanced drought tolerance have been developed to date. Such genetically engineered plants have generally been developed using genes encoding proteins that control drought regulatory networks. These proteins include transcription factors, protein kinases, receptor-like kinases, enzymes related to osmoprotectant or plant hormone synthesis, and other regulatory or functional proteins. Of the drought-tolerant transgenic rice plants described in this review, approximately one-third show decreased plant height under non-stressed conditions or in response to abscisic acid treatment. In cereal crops, plant height is a very important agronomic trait directly affecting yield, although the improvement of lodging resistance should also be taken into consideration. Understanding the regulatory mechanisms of plant growth reduction under drought stress conditions holds promise for developing transgenic plants that produce high yields under drought stress conditions. Plant growth rates are reduced more rapidly than photosynthetic activity under drought conditions, implying that plants actively reduce growth in response to drought stress. In this review, we summarize studies on molecular regulatory networks involved in response to drought stress. In a separate section, we highlight progress in the development of transgenic drought-tolerant rice plants, with special attention paid to field trial investigations.

  3. Regulatory T cells: serious contenders in the promise for immunological tolerance in transplantation

    Directory of Open Access Journals (Sweden)

    Niloufar eSafinia

    2015-08-01

    Full Text Available Regulatory T cells (Tregs play an important role in immunoregulation and have been shown in animal models to promote transplantation tolerance and curb autoimmunity following their adoptive transfer. The safety and potential therapeutic efficacy of these cells has already been reported in Phase I trials of bone marrow transplantation and type I diabetes, the success of which has motivated the broadened application of these cells in solid organ transplantation. Despite major advances in the clinical translation of these cells, there are still key questions to be addressed to ensure that Tregs attest their reputation as ideal candidates for tolerance induction. In this review, we will discuss the unique traits of Tregs that have attracted such fame in the arena of tolerance induction. We will outline the protocols used for their ex vivo expansion and discuss the future directions of Treg cell therapy. In this regard, we will review the concept of Treg heterogeneity, the desire to isolate and expand a functionally superior Treg population and report on the effect of differing culture conditions. The relevance of Treg migratory capacity will also be discussed together with methods of in vivo visualization of the infused cells. Moreover, we will highlight key advances in the identification and expansion of antigen specific Tregs and discuss their significance for cell therapy application. We will also summarize the clinical parameters that are of importance, alongside cell manufacture, from the choice of immunosuppression regimens to the number of injections in order to direct the success of future efficacy trials of Treg cell therapy.Years of research in the field of tolerance have seen an accumulation of knowledge and expertise in the field of Treg biology. This perpetual progression has been the driving force behind the many successes to date and has put us now within touching distance of our ultimate success, immunological tolerance.

  4. Tolerance to noninherited maternal antigens, reproductive microchimerism and regulatory T cell memory: 60 years after ‘Evidence for actively acquired tolerance to Rh antigens’

    Science.gov (United States)

    Kinder, Jeremy M.; Jiang, Tony T.; Ertelt, James M.; Xin, Lijun; Strong, Beverly S.; Shaaban, Aimen F.; Way, Sing Sing

    2015-01-01

    ABSTRACT Compulsory exposure to genetically foreign maternal tissue imprints in offspring sustained tolerance to noninherited maternal antigens (NIMA). Immunological tolerance to NIMA was first described by Dr. Ray D. Owen for women genetically negative for erythrocyte rhesus (Rh) antigen with reduced sensitization from developmental Rh exposure by their mothers. Extending this analysis to HLA haplotypes has uncovered the exciting potential for therapeutically exploiting NIMA-specific tolerance naturally engrained in mammalian reproduction for improved clinical outcomes after allogeneic transplantation. Herein, we summarize emerging scientific concepts stemming from tolerance to NIMA that includes postnatal maintenance of microchimeric maternal origin cells in offspring, expanded accumulation of immune suppressive regulatory T cells with NIMA-specificity, along with teleological benefits and immunological consequences of NIMA-specific tolerance conserved across mammalian species. PMID:26517600

  5. Regulatory issues of digital instrumentation and control system in Lungmen project

    Energy Technology Data Exchange (ETDEWEB)

    Chuang, C.F. [Nuclear Regulation Division, Atomic Energy Council, Taipei (China); Chou, H.P. [Department of Engineering and System Science National Tsing Hua University, (China)

    2004-07-01

    The Lungmen Nuclear Power Station (LNPS) is currently under construction in Taiwan, which consists of 2 advanced boiling water reactor (ABWR) units. The instrumentation and control (IC) systems of the LNPS are based on the state-of-the-art modernized fully integrated digital design. These IC systems possess many advantages and distinguished features comparing to traditional analog IC systems, they enjoy set-point stability, self-diagnostic and automatic testing ability, fault tolerance and avoidance, low power requirements, data handling and storage capability, as well as enhanced human-machine interfaces. This paper presents regulatory overviews, regulatory requirements, current major regulatory issues, as well as the areas of regulatory concerns and the lessons learned on the digital IC systems in the Lungmen Project.

  6. Fault Tolerant Controllers for Sampled-data Systems

    DEFF Research Database (Denmark)

    Niemann, H.; Stoustrup, Jakob

    2004-01-01

    A general compensator architecture for fault tolerant control (FTC) for sampled-data systems is proposed. The architecture is based on the YJBK parameterization of all stabilizing controllers, and uses the dual YJBK parameterization to quantify the performance of the fault tolerant system. The FTC...

  7. Fault tolerant controllers for sampled-data systems

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik; Stoustrup, Jakob

    2004-01-01

    A general compensator architecture for fault tolerant control (FTC) for sampled-data systems is proposed. The architecture is based on the YJBK parameterization of all stabilizing controllers, and uses the dual YJBK parameterization to quantify the performance of the fault tolerant system. The FTC...

  8. Fault tolerant control for uncertain systems with parametric faults

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik; Poulsen, Niels Kjølstad

    2006-01-01

    A fault tolerant control (FTC) architecture based on active fault diagnosis (AFD) and the YJBK (Youla, Jarb, Bongiorno and Kucera)parameterization is applied in this paper. Based on the FTC architecture, fault tolerant control of uncertain systems with slowly varying parametric faults...

  9. Regulatory T cells, maternal-foetal immune tolerance and recurrent miscarriage: new therapeutic challenging opportunities.

    Science.gov (United States)

    Alijotas-Reig, Jaume; Melnychuk, Taisiia; Gris, Josep Maria

    2015-03-15

    Because maternal alloreactive lymphocytes are not depleted during pregnancy, local and/or systemic mechanisms have to play a key role in altering the maternal immune response. Peripheral T regulatory cells (pTregs) at the maternal-foetal interface are necessary in situ to prevent early abortion, but only those pTregs that have been previously exposed to paternal alloantigens. It has been showed that pregnancy selectively stimulates the accumulation of maternal Foxp3(+)CD4(+)CD25(+) (Foxp3Tregs) cells with foetal specificity. Interestingly, after delivery, foetal-specific pTregs persist at elevated levels, maintain tolerance to pre-existing foetal antigen, and rapidly re-accumulate during subsequent pregnancy. pTreg up-regulation could be hypothesized as a possible future therapeutic strategy in humans. Copyright © 2014 Elsevier España, S.L.U. All rights reserved.

  10. Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance.

    Science.gov (United States)

    Chaturvedi, Vandana; Collison, Lauren W; Guy, Clifford S; Workman, Creg J; Vignali, Dario A A

    2011-06-15

    Human regulatory T cells (T(reg)) are essential for the maintenance of immune tolerance. However, the mechanisms they use to mediate suppression remain controversial. Although IL-35 has been shown to play an important role in T(reg)-mediated suppression in mice, recent studies have questioned its relevance in human T(reg). In this study, we show that human T(reg) express and require IL-35 for maximal suppressive capacity. Substantial upregulation of EBI3 and IL12A, but not IL10 and TGFB, was observed in activated human T(reg) compared with conventional T cells (T(conv)). Contact-independent T(reg)-mediated suppression was IL-35 dependent and did not require IL-10 or TGF-β. Lastly, human T(reg)-mediated suppression led to the conversion of the suppressed T(conv) into iTr35 cells, an IL-35-induced T(reg) population, in an IL-35-dependent manner. Thus, IL-35 contributes to human T(reg)-mediated suppression, and its conversion of suppressed target T(conv) into IL-35-induced T(reg) may contribute to infectious tolerance.

  11. New regulatory framework for wastewater quality control in Mexico.

    Science.gov (United States)

    Saade Hazin, L

    2000-01-01

    This paper analyses the new regulatory framework for wastewater quality control developed in Mexico. It provides a description of the Mexican government strategies for water pollution control. The discussion focuses on the policy instruments used, their evolution and the main difficulties encountered in their implementation. The new regulatory framework is discussed highlighting some of the economic implications of the reforms. The paper concludes that proper institutional development and monitoring are essential factors for the success of any policy instrument for wastewater quality control.

  12. Evolutionary analysis and lateral gene transfer of two-component regulatory systems associated with heavy-metal tolerance in bacteria.

    Science.gov (United States)

    Bouzat, Juan L; Hoostal, Matthew J

    2013-05-01

    Microorganisms have adapted intricate signal transduction mechanisms to coordinate tolerance to toxic levels of metals, including two-component regulatory systems (TCRS). In particular, both cop and czc operons are regulated by TCRS; the cop operon plays a key role in bacterial tolerance to copper, whereas the czc operon is involved in the efflux of cadmium, zinc, and cobalt from the cell. Although the molecular physiology of heavy metal tolerance genes has been extensively studied, their evolutionary relationships are not well-understood. Phylogenetic relationships among heavy-metal efflux proteins and their corresponding two-component regulatory proteins revealed orthologous and paralogous relationships from species divergences and ancient gene duplications. The presence of heavy metal tolerance genes on bacterial plasmids suggests these genes may be prone to spread through horizontal gene transfer. Phylogenetic inferences revealed nine potential examples of lateral gene transfer associated with metal efflux proteins and two examples for regulatory proteins. Notably, four of the examples suggest lateral transfer across major evolutionary domains. In most cases, differences in GC content in metal tolerance genes and their corresponding host genomes confirmed lateral gene transfer events. Three-dimensional protein structures predicted for the response regulators encoded by cop and czc operons showed a high degree of structural similarity with other known proteins involved in TCRS signal transduction, which suggests common evolutionary origins of functional phenotypes and similar mechanisms of action for these response regulators.

  13. Fault tolerant control schemes using integral sliding modes

    CERN Document Server

    Hamayun, Mirza Tariq; Alwi, Halim

    2016-01-01

    The key attribute of a Fault Tolerant Control (FTC) system is its ability to maintain overall system stability and acceptable performance in the face of faults and failures within the feedback system. In this book Integral Sliding Mode (ISM) Control Allocation (CA) schemes for FTC are described, which have the potential to maintain close to nominal fault-free performance (for the entire system response), in the face of actuator faults and even complete failures of certain actuators. Broadly an ISM controller based around a model of the plant with the aim of creating a nonlinear fault tolerant feedback controller whose closed-loop performance is established during the design process. The second approach involves retro-fitting an ISM scheme to an existing feedback controller to introduce fault tolerance. This may be advantageous from an industrial perspective, because fault tolerance can be introduced without changing the existing control loops. A high fidelity benchmark model of a large transport aircraft is u...

  14. Fault Tolerant Control: A Simultaneous Stabilization Result

    DEFF Research Database (Denmark)

    Stoustrup, Jakob; Blondel, V.D.

    2004-01-01

    This paper discusses the problem of designing fault tolerant compensators that stabilize a given system both in the nominal situation, as well as in the situation where one of the sensors or one of the actuators has failed. It is shown that such compensators always exist, provided that the system...

  15. Tolerant Mechanism and Chromosome Location of Gene Controlling Sprouting Tolerance in Aegilops Tauschii Cosson

    Institute of Scientific and Technical Information of China (English)

    LAN Xiu-jin; ZHENG You-liang; LIU Deng-cai; WEI Yu-ming; YAN Ze-hong; ZHOU Yong-hong

    2002-01-01

    An artificial amphiploid RSP (2n = 42, AABBDD) between tetraploid landrace Ailanmai(Triticum turgidum L., 2n= 28, AABB) and Aegilops tauschii (DD, 2n = 14) expressed high tolerance to preharvest sprouting which derived from Ae. tauschii. Tolerance to preharvest sprouting of RSP was examined by four ways in six varying periods after anthesis. The germination percentages of preharvest intact spikes were only 6.06 % in its high peak period of germination. Its tolerance was mainly decided by the seed a recessive trait which was controlled by one gene, located on chromosome 2D.

  16. Regulatory Accessibility and Social Influences on State Self-Control

    OpenAIRE

    vanDellen, Michelle R.; Hoyle, Rick H.

    2009-01-01

    The current work examined how social factors influence self-control. Current conceptions of state self-control treat it largely as a function of regulatory capacity. The authors propose that state self-control might also be influenced by social factors because of regulatory accessibility. Studies 1 through 4 provide evidence that individuals’ state self-control is influenced by the trait and state self-control of salient others such that thinking of others with good trait or state self-contro...

  17. Fault tolerance and reliability in integrated ship control

    DEFF Research Database (Denmark)

    Nielsen, Jens Frederik Dalsgaard; Izadi-Zamanabadi, Roozbeh; Schiøler, Henrik

    2002-01-01

    Various strategies for achieving fault tolerance in large scale control systems are discussed. The positive and negative impacts of distribution through network communication are presented. The ATOMOS framework for standardized reliable marine automation is presented along with the corresponding...

  18. Fault tolerance and reliability in integrated ship control

    DEFF Research Database (Denmark)

    Nielsen, Jens Frederik Dalsgaard; Izadi-Zamanabadi, Roozbeh; Schiøler, Henrik

    2002-01-01

    Various strategies for achieving fault tolerance in large scale control systems are discussed. The positive and negative impacts of distribution through network communication are presented. The ATOMOS framework for standardized reliable marine automation is presented along with the corresponding...

  19. A New Approach to Robust and Fault Tolerant Control

    Institute of Scientific and Technical Information of China (English)

    Kemin Zhou

    2005-01-01

    In this paper, we shall summarize a new approach to robust and fault tolerant control proposed recently by the author. This approach is based on a variation of all controller parametrization. This robust and fault-tolerant control design consists of two parts: a nominal performance controller and a robustness controller, and works in such a way that when a component (sensor,actuator, etc.) failure is detected, the controller structure is reconfigured by adding a robustness loop to compensate the fault. We shall illustrate how this strategy works under various situations.

  20. Regulatory T cells as a therapeutic tool to induce solid-organ transplant tolerance: current clinical experiences.

    Science.gov (United States)

    Nikoueinejad, Hassan; Sharif, Mohammad Reza; Amirzargar, Aliakbar; Mirshafiey, Abbas; Einollahi, Behzad

    2013-10-01

    Long-term tolerance is potentially an ideal in organ transplant. Achieving this leads us to eliminate immunosuppressive therapies and their associated side effects. Although most succession in this field belongs to mixed chimerism methods of tolerance induction, regulatory T cells and (T-reg)-based methods also have been demonstrated to prevent organ rejection and lead to transplant tolerance through different mechanisms. In contrast to chimeric protocols (which require bone marrow transplant), T-reg-mediated protocols do not aggressively manipulate blood and the immune system. Most treatment has been done for graft-versus-host disease after hematopoietic stem cell transplant. This review describes different types and mechanisms of action and clinical strategies using T-regs to induce transplant tolerance.

  1. Sensor Fault Tolerant Generic Model Control for Nonlinear Systems

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A modified Strong Tracking Filter (STF) is used to develop a new approach to sensor fault tolerant control. Generic Model Control (GMC) is used to control the nonlinear process while the process runs normally because of its robust control performance. If a fault occurs in the sensor, a sensor bias vector is then introduced to the output equation of the process model. The sensor bias vector is estimated on-line during every control period using the STF. The estimated sensor bias vector is used to develop a fault detection mechanism to supervise the sensors. When a sensor fault occurs, the conventional GMC is switched to a fault tolerant control scheme, which is, in essence, a state estimation and output prediction based GMC. The laboratory experimental results on a three-tank system demonstrate the effectiveness of the proposed Sensor Fault Tolerant Generic Model Control (SFTGMC) approach.

  2. A fault-tolerant intelligent robotic control system

    Science.gov (United States)

    Marzwell, Neville I.; Tso, Kam Sing

    1993-01-01

    This paper describes the concept, design, and features of a fault-tolerant intelligent robotic control system being developed for space and commercial applications that require high dependability. The comprehensive strategy integrates system level hardware/software fault tolerance with task level handling of uncertainties and unexpected events for robotic control. The underlying architecture for system level fault tolerance is the distributed recovery block which protects against application software, system software, hardware, and network failures. Task level fault tolerance provisions are implemented in a knowledge-based system which utilizes advanced automation techniques such as rule-based and model-based reasoning to monitor, diagnose, and recover from unexpected events. The two level design provides tolerance of two or more faults occurring serially at any level of command, control, sensing, or actuation. The potential benefits of such a fault tolerant robotic control system include: (1) a minimized potential for damage to humans, the work site, and the robot itself; (2) continuous operation with a minimum of uncommanded motion in the presence of failures; and (3) more reliable autonomous operation providing increased efficiency in the execution of robotic tasks and decreased demand on human operators for controlling and monitoring the robotic servicing routines.

  3. Controlling the frontier: regulatory T-cells and intestinal homeostasis.

    Science.gov (United States)

    Bollrath, Julia; Powrie, Fiona M

    2013-11-30

    The intestine represents one of the most challenging sites for the immune system as immune cells must be able to mount an efficient response to invading pathogens while tolerating the large number and diverse array of resident commensal bacteria. Foxp3(+) regulatory T-cells (Tregs) play a non-redundant role at maintaining this balance. At the same time Treg cell differentiation and function can be modulated by the intestinal microbiota. In this review, we will discuss effector mechanisms of Treg cells in the intestine and how these cells can be influenced by the intestinal microbiota. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. Regulatory mode preferences for autonomy supporting versus controlling instructional styles.

    Science.gov (United States)

    Pierro, Antonio; Presaghi, Fabio; Higgins, Tory E; Kruglanski, Arie W

    2009-12-01

    Three studies carried out in educational settings examined determinants of teacher's instructional styles and students' degree of satisfaction with the learning climates created by such styles. Based upon regulatory mode theory, Studies 1 and 2 tested the hypotheses that teachers' locomotion orientation will be positively related, and their assessment orientation will be negatively related, to autonomy supportive (vs. controlling) instructional styles. Study 3 tested the hypothesis that students' regulatory mode will exhibit a fit effect with the prevalent learning climate in their school. Participants for Study 1 were 378 teachers (278 females); for Study 2 were 96 teachers (65 females); and for Study 3 were 190 students (all males). Participants completed questionnaires that included measures of teaching styles (Studies 1 and 2), perceived learning climate and satisfaction (Study 3), and regulatory mode orientations (Studies 1 and 3). In Study 2 regulatory mode orientations were experimentally induced. Results confirmed that teachers' autonomy supportive versus controlling styles were positively related to their locomotion orientations and negatively related to their assessment orientation, and that students with a stronger locomotion (vs. assessment) orientation reported a higher level of satisfaction when the learning climate was perceived as autonomy supportive (vs. controlling). The present studies show that teachers' preference for adopting an instructional style is influenced by their regulatory mode orientations, and that the effects of a learning climate on students' satisfaction are contingent on a fit between type of learning climate and students' regulatory mode orientations.

  5. Nonlinear and fault-tolerant flight control using multivariate splines

    NARCIS (Netherlands)

    Tol, H.J.; De Visser, C.C.; Van Kampen, E.J.; Chu, Q.P.

    2015-01-01

    This paper presents a study on fault tolerant flight control of a high performance aircraft using multivariate splines. The controller is implemented by making use of spline model based adaptive nonlinear dynamic inversion (NDI). This method, indicated as SANDI, combines NDI control with nonlinear

  6. Nonlinear and fault-tolerant flight control using multivariate splines

    NARCIS (Netherlands)

    Tol, H.J.; De Visser, C.C.; Van Kampen, E.J.; Chu, Q.P.

    2015-01-01

    This paper presents a study on fault tolerant flight control of a high performance aircraft using multivariate splines. The controller is implemented by making use of spline model based adaptive nonlinear dynamic inversion (NDI). This method, indicated as SANDI, combines NDI control with nonlinear c

  7. Fault tolerant control based on active fault diagnosis

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik

    2005-01-01

    An active fault diagnosis (AFD) method will be considered in this paper in connection with a Fault Tolerant Control (FTC) architecture based on the YJBK parameterization of all stabilizing controllers. The architecture consists of a fault diagnosis (FD) part and a controller reconfiguration (CR...

  8. Concepts and Methods in Fault-tolerant Control

    DEFF Research Database (Denmark)

    Blanke, Mogens; Staroswiecly, M.; Wu, N.E.

    2001-01-01

    in an intelligent way. The aim is to prevent that simple faults develop into serious failure and hence increase plant availability and reduce the risk of safety hazards. Fault-tolerant control merges several disciplines into a common framework to achieve these goals. The desired features are obtained through on......Faults in automated processes will often cause undesired reactions and shut-down of a controlled plant, and the consequences could be damage to technical parts of the plant, to personnel or the environment. Fault-tolerant control combines diagnosis with control methods to handle faults...

  9. Fault tolerant wind speed estimator used in wind turbine controllers

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Stoustrup, Jakob

    2012-01-01

    . In this paper a fault tolerant wind speed estimator is designed based on a set of unknown input observers, each designed to the different sets of non-faulty sensors. Faults in the rotor, generator and wind speed sensors are considered. The designed wind speed estimator is passive tolerant towards faults...... in the wind speed sensors, and faults in the generator and rotor speed sensors are accommodated by an active fault tolerant observer scheme in which the faults are detected and identified, and the observer corresponding to the non-faulty sensors are used. The potential of the scheme is shown by applying......Advanced control schemes can be used to optimize energy production and cost of energy in modern wind turbines. These control schemes most often rely on wind speed estimations. These designs of wind speed estimators are, however, not designed to be fault tolerant towards faults in the used sensors...

  10. Fault tolerant programmable digital attitude control electronics study

    Science.gov (United States)

    Sorensen, A. A.

    1974-01-01

    The attitude control electronics mechanization study to develop a fault tolerant autonomous concept for a three axis system is reported. Programmable digital electronics are compared to general purpose digital computers. The requirements, constraints, and tradeoffs are discussed. It is concluded that: (1) general fault tolerance can be achieved relatively economically, (2) recovery times of less than one second can be obtained, (3) the number of faulty behavior patterns must be limited, and (4) adjoined processes are the best indicators of faulty operation.

  11. Fault-tolerant Control Systems-An Introductory Overview

    Institute of Scientific and Technical Information of China (English)

    Jin Jiang

    2005-01-01

    This paper presents an introductory overview on the development of fault-tolerant control systems. For this reason, the paper is written in a tutorial fashion to summarize some of the important results in this subject area deliberately without going into details in any of them. However, key references are provided from which interested readers can obtain more detailed information on a particular subject. It is necessary to mention that, throughout this paper, no efforts were made to provide an exhaustive coverage on the subject matter. In fact, it is far from it. The paper merely represents the view and experience of its author. It can very well be that some important issues or topics were left out unintentionally. If that is the case, the author sincerely apologizes in advance.After a brief account of fault-tolerant control systems, particularly on the original motivations, and the concept of redundancies, the paper reviews the development of fault-tolerant control systems with highlights to several important issues from a historical perspective. The general approaches to fault-tolerant control has been divided into passive, active, and hybrid approaches. The analysis techniques for active fault-tolerant control systems are also discussed. Practical applications of faulttolerant control are highlighted from a practical and industrial perspective. Finally, some critical issues in this area are discussed as open problems for future research/development in this emerging field.

  12. A benchmark for fault tolerant flight control evaluation

    NARCIS (Netherlands)

    Smaili, H.; Breeman, J.; Lombaerts, T.; Stroosma, O.

    2013-01-01

    A large transport aircraft simulation benchmark (REconfigurable COntrol for Vehicle Emergency Return − RECOVER) has been developed within the GARTEUR (Group for Aeronautical Research and Technology in Europe) Flight Mechanics Action Group 16 (FM-AG(16)) on Fault Tolerant Control (2004 2008) for the

  13. Design of fault tolerant control system for steam generator using

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Myung Ki; Seo, Mi Ro [Korea Electric Power Research Institute, Taejon (Korea, Republic of)

    1998-12-31

    A controller and sensor fault tolerant system for a steam generator is designed with fuzzy logic. A structure of the proposed fault tolerant redundant system is composed of a supervisor and two fuzzy weighting modulators. A supervisor alternatively checks a controller and a sensor induced performances to identify which part, a controller or a sensor, is faulty. In order to analyze controller induced performance both an error and a change in error of the system output are chosen as fuzzy variables. The fuzzy logic for a sensor induced performance uses two variables : a deviation between two sensor outputs and its frequency. Fuzzy weighting modulator generates an output signal compensated for faulty input signal. Simulations show that the proposed fault tolerant control scheme for a steam generator regulates well water level by suppressing fault effect of either controllers or sensors. Therefore through duplicating sensors and controllers with the proposed fault tolerant scheme, both a reliability of a steam generator control and sensor system and that of a power plant increase even more. 2 refs., 9 figs., 1 tab. (Author)

  14. Cold plasma: Quality control and regulatory considerations

    Science.gov (United States)

    In recent years, cold plasma has emerged as a promising antimicrobial treatment for fresh and fresh-cut produce, nuts, spices, seeds, and other foods. Research has demonstrated effective control of human pathogens such as Salmonella, Listeria monocytogenes, Escherichia coli O157:H7, norovirus, and o...

  15. Regulatory perspective on incomplete control rod insertions

    Energy Technology Data Exchange (ETDEWEB)

    Chatterton, M.

    1997-01-01

    The incomplete control rod insertions experienced at South Texas Unit 1 and Wolf Creek are of safety concern to the NRC staff because they represent potential precursors to loss of shutdown margin. Even before it was determined if these events were caused by the control rods or by the fuel there was an apparent correlation of the problem with high burnup fuel. It was determined that there was also a correlation between high burnup and high drag forces as well as with rod drop time histories and lack of rod recoil. The NRC staff initial actions were aimed at getting a perspective on the magnitude of the problem as far as the number of plants and the amount of fuel that could be involved, as well as the safety significance in terms of shutdown margin. As tests have been performed and data has been analyzed the focus has shifted more toward understanding the problem and the ways to eliminate it. At this time the staff`s understanding of the phenomena is that it was a combination of factors including burnup, power history and temperature. The problem appears to be very sensitive to these factors, the interaction of which is not clearly understood. The model developed by Westinghouse provides a possible explanation but there is not sufficient data to establish confidence levels and sensitivity studies involving the key parameters have not been done. While several fixes to the problem have been discussed, no definitive fixes have been proposed. Without complete understanding of the phenomena, or fixes that clearly eliminate the problem the safety concern remains. The safety significance depends on the amount of shutdown margin lost due to incomplete insertion of the control rods. Were the control rods to stick high in the core, the reactor could not be shutdown by the control rods and other means such as emergency boration would be required.

  16. Mine-Hoist Active Fault Tolerant Control System and Strategy

    Institute of Scientific and Technical Information of China (English)

    WANG Zhi-jie; WANG Yao-cai; MENG Jiang; ZHAO Peng-cheng; CHANG Yan-wei

    2005-01-01

    Based on fault diagnosis and fault tolerant technologies, the mine-hoist active fault-tolerant control system (MAFCS) is presented with corresponding strategies,, which includes the fault diagnosis module (FDM), the dynamic library (DL) and the fault-tolerant control module (FCM). When a fault is judged from some sensor by FDM, FCM reconfigure the state of MAFCS by calling the parameters from all sub libraries in DL, in order to ensure the reliability and safety of mine hoist. The simulating result shows that, MAFCS is of certain intelligence, which can adopt the corresponding control strategies according to different fault modes, even when there are quite difference between the real data and the prior fault modes.

  17. Fault Tolerant Control of Wind Turbines

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Stoustrup, Jakob; Kinnaert, Michel

    2013-01-01

    This paper presents a test benchmark model for the evaluation of fault detection and accommodation schemes. This benchmark model deals with the wind turbine on a system level, and it includes sensor, actuator, and system faults, namely faults in the pitch system, the drive train, the generator......, and the converter system. Since it is a system-level model, converter and pitch system models are simplified because these are controlled by internal controllers working at higher frequencies than the system model. The model represents a three-bladed pitch-controlled variable-speed wind turbine with a nominal power...

  18. Robust control of robots fault tolerant approaches

    CERN Document Server

    Siqueira, Adriano A G; Bergerman, Marcel

    2014-01-01

    Bridging the divide between robust control theory and its application, this volume focuses on robotic manipulators and illustrates the mathematical concepts through experimental results in reproducible detail, obtained with a two-manipulator system.

  19. Fault diagnosis and fault-tolerant control based on adaptive control approach

    CERN Document Server

    Shen, Qikun; Shi, Peng

    2017-01-01

    This book provides recent theoretical developments in and practical applications of fault diagnosis and fault tolerant control for complex dynamical systems, including uncertain systems, linear and nonlinear systems. Combining adaptive control technique with other control methodologies, it investigates the problems of fault diagnosis and fault tolerant control for uncertain dynamic systems with or without time delay. As such, the book provides readers a solid understanding of fault diagnosis and fault tolerant control based on adaptive control technology. Given its depth and breadth, it is well suited for undergraduate and graduate courses on linear system theory, nonlinear system theory, fault diagnosis and fault tolerant control techniques. Further, it can be used as a reference source for academic research on fault diagnosis and fault tolerant control, and for postgraduates in the field of control theory and engineering. .

  20. Fault-Tolerant Process Control Methods and Applications

    CERN Document Server

    Mhaskar, Prashant; Christofides, Panagiotis D

    2013-01-01

    Fault-Tolerant Process Control focuses on the development of general, yet practical, methods for the design of advanced fault-tolerant control systems; these ensure an efficient fault detection and a timely response to enhance fault recovery, prevent faults from propagating or developing into total failures, and reduce the risk of safety hazards. To this end, methods are presented for the design of advanced fault-tolerant control systems for chemical processes which explicitly deal with actuator/controller failures and sensor faults and data losses. Specifically, the book puts forward: ·         a framework for  detection, isolation and diagnosis of actuator and sensor faults for nonlinear systems; ·         controller reconfiguration and safe-parking-based fault-handling methodologies; ·         integrated-data- and model-based fault-detection and isolation and fault-tolerant control methods; ·         methods for handling sensor faults and data losses; and ·      ...

  1. Concepts and Methods in Fault-tolerant Control

    DEFF Research Database (Denmark)

    Blanke, Mogens; Staroswiecly, M.; Wu, N.E.

    2001-01-01

    Faults in automated processes will often cause undesired reactions and shut-down of a controlled plant, and the consequences could be damage to technical parts of the plant, to personnel or the environment. Fault-tolerant control combines diagnosis with control methods to handle faults...... in an intelligent way. The aim is to prevent that simple faults develop into serious failure and hence increase plant availability and reduce the risk of safety hazards. Fault-tolerant control merges several disciplines into a common framework to achieve these goals. The desired features are obtained through on...... other situations, complex reconfiguration or on-line controller redesign is required. This paper gives an overview of recent tools to analyze and explore structure and other fundamental properties of an automated system such that any inherent redundancy in the controlled process can be fully utilized...

  2. Flatness-based fault tolerant control

    Directory of Open Access Journals (Sweden)

    César Martínez-Torres

    2014-01-01

    Full Text Available Este artículo presenta un método de control tolerante a fallas para sistemas no lineales planos. Las propiedades intrínsecas d e los sistemas planos generan redundancia analítica y permiten calcular todos los estados y las entradas de control del sistema. Los residuo s son calculados comparando las medidas reales provenientes de los se nsores y las señales obtenidas gracias al conjunto de ecuacione s del sistema plano. Fallas multiplicativas y aditivas se pueden man ejar de manera indistinta. Las señales redundantes obtenidas co n las ecuaciones del sistema plano son usadas para reconfigurar el si stema con falla. La factibilidad del método propuesto es verifi cada para fallas aditivas en un sistema de tres tanques.

  3. Fault Tolerant Control System Design Using Automated Methods from Risk Analysis

    DEFF Research Database (Denmark)

    Blanke, M.

    Fault tolerant controls have the ability to be resilient to simple faults in control loop components.......Fault tolerant controls have the ability to be resilient to simple faults in control loop components....

  4. The changed balance of regulatory and naive T cells promotes tolerance after TLI and anti-T-cell antibody conditioning.

    Science.gov (United States)

    Nador, R G; Hongo, D; Baker, J; Yao, Z; Strober, S

    2010-02-01

    The goal of the study was to determine how the changed balance of host naïve and regulatory T cells observed after conditioning with total lymphoid irradiation (TLI) and antithymocyte serum (ATS) promotes tolerance to combined organ and bone marrow transplants. Although previous studies showed that tolerance was dependent on host natural killer T (NKT) cells, this study shows that there is an additional dependence on host CD4(+)CD25(+) Treg cells. Depletion of the latter cells before conditioning resulted in rapid rejection of bone marrow and organ allografts. The balance of T-cell subsets changed after TLI and ATS with TLI favoring mainly NKT cells and ATS favoring mainly Treg cells. Combined modalities reduced the conventional naïve CD4(+) T cells 2800-fold. The host type Treg cells that persisted in the stable chimeras had the capacity to suppress alloreactivity to both donor and third party cells in the mixed leukocyte reaction. In conclusion, tolerance induction after conditioning in this model depends upon the ability of naturally occurring regulatory NKT and Treg cells to suppress the residual alloreactive T cells that are capable of rejecting grafts.

  5. Noise Control in Gene Regulatory Networks with Negative Feedback.

    Science.gov (United States)

    Hinczewski, Michael; Thirumalai, D

    2016-07-01

    Genes and proteins regulate cellular functions through complex circuits of biochemical reactions. Fluctuations in the components of these regulatory networks result in noise that invariably corrupts the signal, possibly compromising function. Here, we create a practical formalism based on ideas introduced by Wiener and Kolmogorov (WK) for filtering noise in engineered communications systems to quantitatively assess the extent to which noise can be controlled in biological processes involving negative feedback. Application of the theory, which reproduces the previously proven scaling of the lower bound for noise suppression in terms of the number of signaling events, shows that a tetracycline repressor-based negative-regulatory gene circuit behaves as a WK filter. For the class of Hill-like nonlinear regulatory functions, this type of filter provides the optimal reduction in noise. Our theoretical approach can be readily combined with experimental measurements of response functions in a wide variety of genetic circuits, to elucidate the general principles by which biological networks minimize noise.

  6. Regulatory role of DREB transcription factors in plant drought, salt and cold tolerance

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    rd29A gene of Arabidopsis encodes a LEA-like hydrophilic protein, its expression is induced by drought, high-salt and cold stress. In the promoter region of rd29A gene, there are 2 DRE cis-acting elements involved in responses to these environmental stresses. 5 cDNAs (DREB1A~C and DREB2A~B) encoding DREB transcription factors, which specifically bind to DRE element and control the expression of reporter gene under drought, high-salt and stress, have been isolated by One-Hybrid screening method and with DRE element of rd29A promoter. DREB transcription factors and DRE element function in signal transduction of drought, high-salt and cold stress. One DREB transcription factor can control the expression of several target functional genes involved in plant tolerance to drought, high-salt and cold stress. Thus, it may be an effective strategy to achieve ideal, multiple and fundamental effect for improving plant stress-resistance by DREB gene transfer.

  7. A benchmark for fault tolerant flight control evaluation

    Science.gov (United States)

    Smaili, H.; Breeman, J.; Lombaerts, T.; Stroosma, O.

    2013-12-01

    A large transport aircraft simulation benchmark (REconfigurable COntrol for Vehicle Emergency Return - RECOVER) has been developed within the GARTEUR (Group for Aeronautical Research and Technology in Europe) Flight Mechanics Action Group 16 (FM-AG(16)) on Fault Tolerant Control (2004 2008) for the integrated evaluation of fault detection and identification (FDI) and reconfigurable flight control strategies. The benchmark includes a suitable set of assessment criteria and failure cases, based on reconstructed accident scenarios, to assess the potential of new adaptive control strategies to improve aircraft survivability. The application of reconstruction and modeling techniques, based on accident flight data, has resulted in high-fidelity nonlinear aircraft and fault models to evaluate new Fault Tolerant Flight Control (FTFC) concepts and their real-time performance to accommodate in-flight failures.

  8. Tolerance

    DEFF Research Database (Denmark)

    Tønder, Lars

    Tolerance: A Sensorial Orientation to Politics is an experiment in re-orientation. The book is based on the wager that tolerance exceeds the more prevalent images of self-restraint and repressive benevolence because neither precludes the possibility of a more “active tolerance” motivated by the d...... these alternatives by returning to the notion of tolerance as the endurance of pain, linking this notion to exemplars and theories relevant to the politics of multiculturalism, religious freedom, and free speech....

  9. Tolerance

    DEFF Research Database (Denmark)

    Tønder, Lars

    Tolerance: A Sensorial Orientation to Politics is an experiment in re-orientation. The book is based on the wager that tolerance exceeds the more prevalent images of self-restraint and repressive benevolence because neither precludes the possibility of a more “active tolerance” motivated by the d...... these alternatives by returning to the notion of tolerance as the endurance of pain, linking this notion to exemplars and theories relevant to the politics of multiculturalism, religious freedom, and free speech....

  10. Data Driven Fault Tolerant Control: A Subspace Approach

    NARCIS (Netherlands)

    Dong, J.

    2009-01-01

    The main stream research on fault detection and fault tolerant control has been focused on model based methods. As far as a model is concerned, changes therein due to faults have to be extracted from measured data. Generally speaking, existing approaches process measured inputs and outputs either by

  11. Diagnosis and Fault-tolerant Control, 3rd Edition

    DEFF Research Database (Denmark)

    Blanke, Mogens; Kinnaert, Michel; Lunze, Jan;

    The book presents effective model-based analysis and design methods for fault diagnosis and fault-tolerant control. Architectural and structural models are used to analyse the propagation of the fault through the process, to test the fault detectability and to find the redundancies in the process...

  12. A Fault tolerant Control Supervisory System development Procedurefor Small Satellites

    DEFF Research Database (Denmark)

    Izadi-Zamanabadi, Roozbeh; Larsen, Jesper Abildgaard

    The paper presents a stepwise procedure to develop a fault tolerant control system for small satellites. The procedure is illustrated through implementation on the AAUSAT-II spacecraft. As it is shown the presented procedure requires expertise from several disciplines that are nevertheless...

  13. Wind turbine fault detection and fault tolerant control

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Johnson, Kathryn

    2013-01-01

    In this updated edition of a previous wind turbine fault detection and fault tolerant control challenge, we present a more sophisticated wind turbine model and updated fault scenarios to enhance the realism of the challenge and therefore the value of the solutions. This paper describes the challe...

  14. A model–based approach to fault–tolerant control

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik

    2012-01-01

    reconfiguration part. The theoretical basis for the architecture is given followed by an investigation of the single parts in the architecture. It is shown that the general controller parameterization is central in connection with both fault diagnosis as well as controller reconfiguration. Especially in relation......A model-based controller architecture for Fault-Tolerant Control (FTC) is presented in this paper. The controller architecture is based on a general controller parameterization. The FTC architecture consists of two main parts, a Fault Detection and Isolation (FDI) part and a controller...... to the controller reconfiguration part, the application of controller parameterization results in a systematic technique for switching between different controllers. This also allows controller switching using different sets of actuators and sensors....

  15. Pancreatic islets engineered with SA-FasL protein establish robust localized tolerance by inducing regulatory T cells in mice.

    Science.gov (United States)

    Yolcu, Esma S; Zhao, Hong; Bandura-Morgan, Laura; Lacelle, Chantale; Woodward, Kyle B; Askenasy, Nadir; Shirwan, Haval

    2011-12-01

    Allogeneic islet transplantation is an important therapeutic approach for the treatment of type 1 diabetes. Clinical application of this approach, however, is severely curtailed by allograft rejection primarily initiated by pathogenic effector T cells regardless of chronic use of immunosuppression. Given the role of Fas-mediated signaling in regulating effector T cell responses, we tested if pancreatic islets can be engineered ex vivo to display on their surface an apoptotic form of Fas ligand protein chimeric with streptavidin (SA-FasL) and whether such engineered islets induce tolerance in allogeneic hosts. Islets were modified with biotin following efficient engineering with SA-FasL protein that persisted on the surface of islets for >1 wk in vitro. SA-FasL-engineered islet grafts established euglycemia in chemically diabetic syngeneic mice indefinitely, demonstrating functionality and lack of acute toxicity. Most importantly, the transplantation of SA-FasL-engineered BALB/c islet grafts in conjunction with a short course of rapamycin treatment resulted in robust localized tolerance in 100% of C57BL/6 recipients. Tolerance was initiated and maintained by CD4(+)CD25(+)Foxp3(+) regulatory T (Treg) cells, as their depletion early during tolerance induction or late after established tolerance resulted in prompt graft rejection. Furthermore, Treg cells sorted from graft-draining lymph nodes, but not spleen, of long-term graft recipients prevented the rejection of unmodified allogeneic islets in an adoptive transfer model, further confirming the Treg role in established tolerance. Engineering islets ex vivo in a rapid and efficient manner to display on their surface immunomodulatory proteins represents a novel, safe, and clinically applicable approach with important implications for the treatment of type 1 diabetes.

  16. Pancreatic Islets Engineered with SA-FasL Protein Establish Robust Localized Tolerance by Inducing T Regulatory Cells in Mice

    Science.gov (United States)

    Yolcu, Esma S; Zhao, Hong; Bandura-Morgan, Laura; Lacelle, Chantale; Woodward, Kyle B; Askenasy, Nadir; Shirwan, Haval

    2011-01-01

    Allogeneic islet transplantation is an important therapeutic approach for the treatment of T1D. Clinical application of this approach, however, is severely curtailed by allograft rejection primarily initiated by pathogenic T effector cells regardless of chronic use of immunosuppression. Given the role of Fas-mediated signaling in regulating T effector cell responses, we tested if pancreatic islets can be engineered ex vivo to display on their surface an apoptotic form of FasL protein chimeric with streptavidin (SA-FasL), and whether such engineered islets induce tolerance in allogeneic hosts. Islets were modified with biotin following efficient engineering with SA-FasL protein that persisted on the surface of islets for over a week in vitro. SA-FasL-engineered islet grafts established euglycemia in chemically diabetic syngeneic mice indefinitely, demonstrating functionality and lack of acute toxicity. Most importantly, the transplantation of SA-FasL-engineered BALB/c islet grafts in conjunction with a short course of rapamycin treatment resulted in robust localized tolerance in 100% C57BL/6 recipients. Tolerance was initiated and maintained by CD4+CD25+FoxP3+ T regulatory (Treg) cells as their depletion early during tolerance induction or late after established tolerance resulted in prompt graft rejection. Furthermore, Treg cells sorted from graft-draining lymph nodes, but not spleen, of long-term graft recipients prevented the rejection of unmodified allogeneic islets in an adoptive transfer model, further confirming the Treg role in established tolerance. Engineering islets ex vivo in a rapid and efficient manner to display on their surface immunomodulatory proteins represents a novel, safe, and clinically applicable approach with important implications for the treatment of T1D. PMID:22068235

  17. The importance of CD25+CD4+ regulatory T cells in mouse hepatic allograft tolerance

    National Research Council Canada - National Science Library

    Jiang, Xiaofeng; Morita, Miwa; Sugioka, Atsushi; Harada, Michishige; Kojo, Satoshi; Wakao, Hiroshi; Watarai, Hiroshi; Ohkohchi, Nobuhiro; Taniguchi, Masaru; Seino, Ken‐ichiro

    2006-01-01

    .... Furthermore, the number of CD25 + CD4 + population and forkhead/winged helix transcription factor expression in liver mononuclear lymphocytes derived from tolerant mice were higher than those from grafts undergoing rejection...

  18. Fault Tolerant Robust Control Applied for Induction Motor (LMI approach

    Directory of Open Access Journals (Sweden)

    Hamouda KHECHINI

    2007-09-01

    Full Text Available This paper foregrounds fault tolerant robust control of uncertain dynamic linear systems in the state space representation. In fact, the industrial systems are more and more complex and the diagnosis process becomes indispensable to guarantee their surety of functioning and availability, that’s why a fault tolerant control law is imperative to achieve the diagnosis. In this paper, we address the problem of state feedback H2 /H∞ mixed with regional pole placement for linear continuous uncertain system. Sufficient conditions for feasibility are derived for a general class of convex regions of the complex plan. The conditions are presented as a collection of linear matrix inequalities (LMI 's. The efficiency and performance of this approach are then tested taking into consideration the robust control of a three- phase induction motor drive with the fluctuation of its parameters during the functioning.

  19. Passive Fault tolerant Control of an Inverted Double Pendulum

    DEFF Research Database (Denmark)

    Niemann, H.; Stoustrup, Jakob

    2003-01-01

    A passive fault tolerant control scheme is suggested, in which a nominal controller is augmented with an additional block, which guarantees stability and performance after the occurrence of a fault. The method is based on the Youla parameterization, which requires the nominal controller to be imp...... to be implemented in the observer based form. The proposed method is applied to a double inverted pendulum system, for which an H controller has been designed and verified in a lap setup. In this case study, the fault is a degradation of the tacho loop....

  20. Infection control and hazards management. Economics of regulatory compliance.

    Science.gov (United States)

    Runnells, R R

    1991-04-01

    Dentistry has become subject to rapid change in office safety, including infection control and hazards management. This change includes increasingly diverse governmental regulations and compliance with such regulations, influencing the very basics of dental practice. As all practitioners are moving toward compliance, costs are increasing substantially. Various sources estimate such increases at between 12.5% and 19%, and it is doubtful whether third-party reimbursement will offset these additional costs. As practitioners plan methods for offsetting the costs of office safety, consideration should be given to providing patients oral and printed information to preclude misinterpretation of the reasons for fee escalation caused by implementation of chemical hazards communication, infection control, and waste disposal programs mandated by OSHA, EPA, and state or other regulatory authorities. The decade of the 1990s may well become the period of meeting the formidable microbiological and regulatory challenges of the 1980s.

  1. Overexpression of pigeonpea stress-induced cold and drought regulatory gene (CcCDR) confers drought, salt, and cold tolerance in Arabidopsis.

    Science.gov (United States)

    Tamirisa, Srinath; Vudem, Dashavantha Reddy; Khareedu, Venkateswara Rao

    2014-09-01

    A potent cold and drought regulatory protein-encoding gene (CcCDR) was isolated from the subtractive cDNA library of pigeonpea plants subjected to drought stress. CcCDR was induced by different abiotic stress conditions in pigeonpea. Overexpression of CcCDR in Arabidopsis thaliana imparted enhanced tolerance against major abiotic stresses, namely drought, salinity, and low temperature, as evidenced by increased biomass, root length, and chlorophyll content. Transgenic plants also showed increased levels of antioxidant enzymes, proline, and reducing sugars under stress conditions. Furthermore, CcCDR-transgenic plants showed enhanced relative water content, osmotic potential, and cell membrane stability, as well as hypersensitivity to abscisic acid (ABA) as compared with control plants. Localization studies confirmed that CcCDR could enter the nucleus, as revealed by intense fluorescence, indicating its possible interaction with various nuclear proteins. Microarray analysis revealed that 1780 genes were up-regulated in CcCDR-transgenics compared with wild-type plants. Real-time PCR analysis on selected stress-responsive genes, involved in ABA-dependent and -independent signalling networks, revealed higher expression levels in transgenic plants, suggesting that CcCDR acts upstream of these genes. The overall results demonstrate the explicit role of CcCDR in conferring multiple abiotic stress tolerance at the whole-plant level. The multifunctional CcCDR seems promising as a prime candidate gene for enhancing abiotic stress tolerance in diverse plants.

  2. Fault tolerance control for proton exchange membrane fuel cell systems

    Science.gov (United States)

    Wu, Xiaojuan; Zhou, Boyang

    2016-08-01

    Fault diagnosis and controller design are two important aspects to improve proton exchange membrane fuel cell (PEMFC) system durability. However, the two tasks are often separately performed. For example, many pressure and voltage controllers have been successfully built. However, these controllers are designed based on the normal operation of PEMFC. When PEMFC faces problems such as flooding or membrane drying, a controller with a specific design must be used. This paper proposes a unique scheme that simultaneously performs fault diagnosis and tolerance control for the PEMFC system. The proposed control strategy consists of a fault diagnosis, a reconfiguration mechanism and adjustable controllers. Using a back-propagation neural network, a model-based fault detection method is employed to detect the PEMFC current fault type (flooding, membrane drying or normal). According to the diagnosis results, the reconfiguration mechanism determines which backup controllers to be selected. Three nonlinear controllers based on feedback linearization approaches are respectively built to adjust the voltage and pressure difference in the case of normal, membrane drying and flooding conditions. The simulation results illustrate that the proposed fault tolerance control strategy can track the voltage and keep the pressure difference at desired levels in faulty conditions.

  3. Active Fault Tolerant Control of Livestock Stable Ventilation System

    DEFF Research Database (Denmark)

    Gholami, Mehdi

    2011-01-01

    of fault. Designing a fault tolerant control scheme for the climate control system. In the first step, a conceptual multi-zone model for climate control of a live-stock building is derived. The model is a nonlinear hybrid model. Hybrid systems contain both discrete and continuous components. The parameters......Modern stables and greenhouses are equipped with different components for providing a comfortable climate for animals and plant. A component malfunction may result in loss of production. Therefore, it is desirable to design a control system, which is stable, and is able to provide an acceptable...... degraded performance even in the faulty case. In this thesis, we have designed such controllers for climate control systems for livestock buildings in three steps: Deriving a model for the climate control system of a pig-stable. Designing a active fault diagnosis (AFD) algorithm for different kinds...

  4. Implications of legalising euthanasia in The Netherlands: greater regulatory control?

    Science.gov (United States)

    Thynne, Kelisiana

    2002-11-01

    Euthanasia has been decriminalised in The Netherlands for the last 30 years, but it was only in April 2001 that the Dutch Parliament legalised voluntary physician-assisted suicide. The legislation incorporates developments in case law, including scope for peer review, and provides common ethical principles for a clear system of control over doctors' actions in regard to patients who request euthanasia. The legalised regulatory system may prove more effective in controlling voluntary and non-voluntary euthanasia in The Netherlands than in countries where euthanasia remains illegal.

  5. Optimal finite horizon control in gene regulatory networks

    Science.gov (United States)

    Liu, Qiuli

    2013-06-01

    As a paradigm for modeling gene regulatory networks, probabilistic Boolean networks (PBNs) form a subclass of Markov genetic regulatory networks. To date, many different stochastic optimal control approaches have been developed to find therapeutic intervention strategies for PBNs. A PBN is essentially a collection of constituent Boolean networks via a probability structure. Most of the existing works assume that the probability structure for Boolean networks selection is known. Such an assumption cannot be satisfied in practice since the presence of noise prevents the probability structure from being accurately determined. In this paper, we treat a case in which we lack the governing probability structure for Boolean network selection. Specifically, in the framework of PBNs, the theory of finite horizon Markov decision process is employed to find optimal constituent Boolean networks with respect to the defined objective functions. In order to illustrate the validity of our proposed approach, an example is also displayed.

  6. A fault tolerant superheat control strategy for supermarket refrigeration systems

    DEFF Research Database (Denmark)

    Vinther, Kasper; Izadi-Zamanabadi, Roozbeh; Rasmussen, Henrik

    2013-01-01

    In this paper, a fault tolerant control (FTC) strategy is proposed for evaporator superheat control in supermarket refrigeration systems. Conventional control uses a pressure and temperature sensor for this purpose, however, the pressure sensor can fail to function. A contingency control strategy......, based on a maximum slope-seeking control method and only a single temperature sensor, is developed to drive the evaporator outlet temperature to a level that gives a suitable superheat of the refrigerant. The FTC strategy requires no a priori system knowledge or additional hardware and functions...... in a plug & play fashion. The strategy is outlined by means of procedural steps as well as a flow chart that also illustrates the process of automatic tuning of the maximum slope-seeking controller. Test results are furthermore presented for a display case in a full scale CO2 supermarket refrigeration...

  7. Regulatory control of nuclear safety in Finland. Annual report 1997

    Energy Technology Data Exchange (ETDEWEB)

    Tossavainen, K. [ed.

    1998-08-01

    The report describes regulatory control of the use of nuclear energy by the Radiation and Nuclear Safety Authority (STUK) in Finland in 1997. Nuclear regulatory control ascertained that the operation of Finnish NPPs was in compliance with the conditions set out in operating licences and current regulations. In addition to NPP normal operation, STUK oversaw projects at the plant units relating to power uprating and safety improvements. STUK prepared statements for the Ministry of Trade and Industry about the applications for renewing the operating licenses of Loviisa and Olkiluoto NPPs. The most important items of supervision in nuclear waste management were studies relating to the final disposal of spent fuel from NPPs and the review of the licence application for a repository for low- and intermediate-level reactor waste from Loviisa NPP. Preparation of general safety regulations for the final disposal of spent nuclear fuel, to be published in the form of a Council of State Decision, was started. By safeguards control, the use of nuclear materials was verified to be in compliance with current regulations and that the whereabouts of every batch of nuclear material were always known. Nuclear material safeguards were stepped up to prevent illicit trafficking of nuclear materials and other radioactive materials. In co-operation with the Ministry for Foreign Affairs and the Institute of Seismology (University of Helsinki), preparations were undertaken to implement the Comprehensive Nuclear Test Ban Treaty (CTBT). For enforcement of the Treaty and as part of the international regulatory approach, STUK is currently developing laboratory analyses relating to airborne radioactivity measurements. The focus of co-operation funded by external sources was as follows: improvement of the safety of Kola and Leningrad NPPs, improvement of nuclear waste management in North-West Russia, development of the organizations of nuclear safety authorities in Eastern Europe and development

  8. A Benchmark Evaluation of Fault Tolerant Wind Turbine Control Concepts

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Stoustrup, Jakob

    2015-01-01

    As the world’s power supply to a larger and larger degree depends on wind turbines, it is consequently and increasingly important that these are as reliable and available as possible. Modern fault tolerant control (FTC) could play a substantial part in increasing reliability of modern wind turbin...... accommodation is handled in software sensor and actuator blocks. This means that the wind turbine controller can continue operation as in the fault free case. The other two evaluated solutions show some potential but probably need improvements before industrial applications....

  9. Regulatory control of genetically modified (GM) foods: likely developments.

    Science.gov (United States)

    Schilter, Benoît; Constable, Anne

    2002-02-28

    The placing of genetically modified (GM) crops on the European market requires a regulatory approval supported by a thorough safety evaluation. This approach has been applied to all GM crops presently on the market. Despite this stringent process there has been an increasing public concern about the impact of GM foods on human health and the environment. In this context, regulatory control may develop in several directions. One response to the public concern is to strengthen the data requirements for the risk assessment process. Several avenues have been proposed. They include the application of technologies such as proteomics and metabolomics to assess unintended changes, and the development of predictive methods to evaluate allergenicity. Obligations for post-launch surveillance have appeared in regulations. Criteria are required to define when and why such approaches are necessary. Significant challenges including feasibility and validation of the methods, and safety relevance of the data generated will have to be addressed before any general application of these new approaches. Effective monitoring requires the ability to identify the presence of GM products and trace their origin. Traceability and labeling are therefore important developments in the GM food regulatory arena. Both require the development of reliable analytical detection tools.

  10. Cpeb4-mediated translational regulatory circuitry controls terminal erythroid differentiation.

    Science.gov (United States)

    Hu, Wenqian; Yuan, Bingbing; Lodish, Harvey F

    2014-09-29

    While we have considerable understanding of the transcriptional networks controlling mammalian cell differentiation, our knowledge of posttranscriptional regulatory events is very limited. Using differentiation of primary erythroid cells as a model, we show that the sequence-specific mRNA-binding protein Cpeb4 is strongly induced by the erythroid-important transcription factors Gata1 and Tal1 and is essential for terminal erythropoiesis. By interacting with the translation initiation factor eIF3, Cpeb4 represses the translation of a large set of mRNAs, including its own mRNA. Thus, transcriptional induction and translational repression combine to form a negative feedback loop to control Cpeb4 protein levels within a specific range that is required for terminal erythropoiesis. Our study provides an example of how translational control is integrated with transcriptional regulation to precisely control gene expression during mammalian cell differentiation.

  11. Regulatory control of nuclear safety in Finland. Annual report 1998

    Energy Technology Data Exchange (ETDEWEB)

    Tossavainen, K. [ed.

    1999-10-01

    The report describes regulatory control of the safe use of nuclear energy by the Radiation and Nuclear Safety Authority (STUK) in 1998. STUK is the Finnish nuclear safety authority. The submission of this report to the Ministry of Trade and Industry is stipulated in Section 121 of the Nuclear Energy Decree. It was verified by regulatory control that the operation of Finnish NPPs was in compliance with conditions set out in the operating licences of the plants and with regulations currently in force. In addition to supervising the normal operation of the plants, STUK oversaw projects carried out at the plant units, which related to the uprating of their power and the improvement of their safety. STUK issued to the Ministry of Trade and Industry a statement about applications for the renewal of the operating licences of Loviisa and Olkiluoto NPPs, which had been submitted by Imatran Voima Oy and Teollisuuden Voima Oy. Regulatory activities in the field of nuclear waste management were focused on the storage and final disposal of spent fuel as well as the treatment, storage and final disposal of reactor waste. STUK issued a statement to the Ministry of Trade and Industry about an environmental impact assessment programme pertaining to a spent fuel repository project, which had been submitted by Posiva Oy, as well as on Imatran Voima Oy's application concerning the operation of a repository for medium- and low-level reactor waste from Loviisa NPP. The use of nuclear materials was in compliance with the regulations currently in force and also the whereabouts of every batch of nuclear material were ensured by safeguards control. In international safeguards, important changes took place, which were reflected also in safeguards activities at national level. International co-operation continued based on financing both from STUK's budget and from additional sources. The focus of co-operation funded from outside sources was as follows: improvement of the safety of

  12. Piecewise Sliding Mode Decoupling Fault Tolerant Control System

    Directory of Open Access Journals (Sweden)

    Rafi Youssef

    2010-01-01

    Full Text Available Problem statement: Proposed method in the present study could deal with fault tolerant control system by using the so called decentralized control theory with decoupling fashion sliding mode control, dealing with subsystems instead of whole system and to the knowledge of the author there is no known computational algorithm for decentralized case, Approach: In this study we present a decoupling strategy based on the selection of sliding surface, which should be in piecewise sliding surface partition to apply the PwLTool which have as purpose in our case to delimit regions where sliding mode occur, after that as Results: We get a simple linearized model selected in those regions which could depict the complex system, Conclusion: With the 3 water tank level system as example we implement this new design scenario and since we are interested in networked control system we believe that this kind of controller implementation will not be affected by network delays.

  13. Human regulatory B cells control the TFH cell response.

    Science.gov (United States)

    Achour, Achouak; Simon, Quentin; Mohr, Audrey; Séité, Jean-François; Youinou, Pierre; Bendaoud, Boutahar; Ghedira, Ibtissem; Pers, Jacques-Olivier; Jamin, Christophe

    2017-07-01

    Follicular helper T (TFH) cells support terminal B-cell differentiation. Human regulatory B (Breg) cells modulate cellular responses, but their control of TFH cell-dependent humoral immune responses is unknown. We sought to assess the role of Breg cells on TFH cell development and function. Human T cells were polyclonally stimulated in the presence of IL-12 and IL-21 to generate TFH cells. They were cocultured with B cells to induce their terminal differentiation. Breg cells were included in these cultures, and their effects were evaluated by using flow cytometry and ELISA. B-cell lymphoma 6, IL-21, inducible costimulator, CXCR5, and programmed cell death protein 1 (PD-1) expressions increased on stimulated human T cells, characterizing TFH cell maturation. In cocultures they differentiated B cells into CD138(+) plasma and IgD(-)CD27(+) memory cells and triggered immunoglobulin secretions. Breg cells obtained by Toll-like receptor 9 and CD40 activation of B cells prevented TFH cell development. Added to TFH cell and B-cell cocultures, they inhibited B-cell differentiation, impeded immunoglobulin secretions, and expanded Foxp3(+)CXCR5(+)PD-1(+) follicular regulatory T cells. Breg cells modulated IL-21 receptor expressions on TFH cells and B cells, and their suppressive activities involved CD40, CD80, CD86, and intercellular adhesion molecule interactions and required production of IL-10 and TGF-β. Human Breg cells control TFH cell maturation, expand follicular regulatory T cells, and inhibit the TFH cell-mediated antibody secretion. These novel observations demonstrate a role for the Breg cell in germinal center reactions and suggest that deficient activities might impair the TFH cell-dependent control of humoral immunity and might lead to the development of aberrant autoimmune responses. Copyright © 2016 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  14. Specific Control of Immunity by Regulatory CD8 T Cells

    Institute of Scientific and Technical Information of China (English)

    XiaoleiTang; TrevorRFSmith

    2005-01-01

    T lymphocytes with dedicated suppressor function (Treg) play a crucial role in the homeostatic control of immunity in the periphery. Several Treg phenotypes have now been identified in the CD4 and CD8 T cell populations, suggesting their down-regulatory function in both human and animal models of autoimmunity, transplantation and tumor immunity. Here we will focus on the CD8 Treg population and their ability to specifically inhibit a pathogenic autoimmune response. This review will detail the current advances in the knowledge of CD8 Treg in the context of antigen specificity, phenotype, MHC restriction, mechanism of action, and priming. Cellular & Molecular Immunology. 2005;2(1):11-19.

  15. Specific Control of Immunity by Regulatory CD8 T Cells

    Institute of Scientific and Technical Information of China (English)

    Xiaolei Tang; Trevor RF Smith; Vipin Kumar

    2005-01-01

    T lymphocytes with dedicated suppressor function (Treg) play a crucial role in the homeostatic control of immunity in the periphery. Several Treg phenotypes have now been identified in the CD4 and CD8 T cell populations,suggesting their down-regulatory function in both human and animal models of autoimmunity, transplantation and tumor immunity. Here we will focus on the CD8 Treg population and their ability to specifically inhibit a pathogenic autoimmune response. This review will detail the current advances in the knowledge of CD8 Treg in the context of antigen specificity, phenotype, MHC restriction, mechanism of action, and priming.

  16. Alloreactive regulatory T cells allow the generation of mixed chimerism and transplant tolerance

    Directory of Open Access Journals (Sweden)

    Paulina eRuiz

    2015-11-01

    Full Text Available The induction of donor-specific transplant tolerance is one of the main goals of modern immunology. Establishment of a mixed chimerism state in the transplant recipient has proven to be a suitable strategy for the induction of long-term allograft tolerance; however, current experimental recipient preconditioning protocols have many side effects, and are not feasible for use in future therapies. In order to improve the current mixed chimerism induction protocols, we developed a non-myeloablative bone-marrow transplant protocol using retinoic acid induced alloantigen-specific Tregs, clinically available immunosuppressive drugs and lower doses of irradiation. We demonstrate that retinoic acid induced alloantigen-specific Tregs in addition to a non-myeloablative bone-marrow transplant protocol generates stable mixed chimerism and induce tolerance to allogeneic secondary skin allografts in mice. Therefore, the establishment of mixed chimerism through the use of donor-specific Tregs rather than non-specific immunosuppression could have a potential use in organ transplantation.

  17. Fault Tolerant Control for Civil Structures Based on LMI Approach

    Directory of Open Access Journals (Sweden)

    Chunxu Qu

    2013-01-01

    Full Text Available The control system may lose the performance to suppress the structural vibration due to the faults in sensors or actuators. This paper designs the filter to perform the fault detection and isolation (FDI and then reforms the control strategy to achieve the fault tolerant control (FTC. The dynamic equation of the structure with active mass damper (AMD is first formulated. Then, an estimated system is built to transform the FDI filter design problem to the static gain optimization problem. The gain is designed to minimize the gap between the estimated system and the practical system, which can be calculated by linear matrix inequality (LMI approach. The FDI filter is finally used to isolate the sensor faults and reform the FTC strategy. The efficiency of FDI and FTC is validated by the numerical simulation of a three-story structure with AMD system with the consideration of sensor faults. The results show that the proposed FDI filter can detect the sensor faults and FTC controller can effectively tolerate the faults and suppress the structural vibration.

  18. Regulatory control analysis and design for sewer systems

    DEFF Research Database (Denmark)

    Mollerup, Ane Loft; Mikkelsen, Peter Steen; Thornberg, Dines

    2015-01-01

    . To this end, four distinct modes of operation are identified (dry weather, filling, saturation and emptying) and for each of these the process gain matrix is found. Based on the gain matrices a controllability analysis is performed, to screen for suitable pairings between measurements and actuators......A systematic methodology for regulatory control analysis and design is adapted for sewer system operation and evaluated. The main challenge with adapting the methodology is the handling of the stochastic and transient nature of the rainfall disturbances, inherent to sewer system operation...... in the case study area of Copenhagen. The analysis effectively reduces the number of potential controlled variables, by considering the sensitivity of the measurements towards changes in the manipulated variables. Several potential pairings are generated and the best alternative is chosen for closed...

  19. H∞ robust fault-tolerant controller design for an autonomous underwater vehicle's navigation control system

    Science.gov (United States)

    Cheng, Xiang-Qin; Qu, Jing-Yuan; Yan, Zhe-Ping; Bian, Xin-Qian

    2010-03-01

    In order to improve the security and reliability for autonomous underwater vehicle (AUV) navigation, an H∞ robust fault-tolerant controller was designed after analyzing variations in state-feedback gain. Operating conditions and the design method were then analyzed so that the control problem could be expressed as a mathematical optimization problem. This permitted the use of linear matrix inequalities (LMI) to solve for the H∞ controller for the system. When considering different actuator failures, these conditions were then also mathematically expressed, allowing the H∞ robust controller to solve for these events and thus be fault-tolerant. Finally, simulation results showed that the H∞ robust fault-tolerant controller could provide precise AUV navigation control with strong robustness.

  20. Controllability analysis of transcriptional regulatory networks reveals circular control patterns among transcription factors

    DEFF Research Database (Denmark)

    Österlund, Tobias; Bordel, Sergio; Nielsen, Jens

    2015-01-01

    Transcriptional regulation is the most committed type of regulation in living cells where transcription factors (TFs) control the expression of their target genes and TF expression is controlled by other TFs forming complex transcriptional regulatory networks that can be highly interconnected. Here...... we analyze the topology and organization of nine transcriptional regulatory networks for E. coli, yeast, mouse and human, and we evaluate how the structure of these networks influences two of their key properties, namely controllability and stability. We calculate the controllability for each network...... as a measure of the organization and interconnectivity of the network. We find that the number of driver nodes n(D) needed to control the whole network is 64% of the TFs in the E. coli transcriptional regulatory network in contrast to only 17% for the yeast network, 4% for the mouse network and 8...

  1. Validation of Gene Regulatory Network Inference Based on Controllability

    Directory of Open Access Journals (Sweden)

    Edward eDougherty

    2013-12-01

    Full Text Available There are two distinct issues regarding network validation: (1 Does an inferred network provide good predictions relative to experimental data? (2 Does a network inference algorithm applied within a certain network model framework yield networks that are accurate relative to some criterion of goodness? The first issue concerns scientific validation and the second concerns algorithm validation. In this paper we consider inferential validation relative to controllability; that is, if an inference procedure is applied to synthetic data generated from a gene regulatory network and an intervention procedure is designed on the inferred network, how well does it perform on the true network? The reasoning behind such a criterion is that, if our purpose is to use gene regulatory networks to design therapeutic intervention strategies, then we are not concerned with network fidelity, per se, but only with our ability to design effective interventions based on the inferred network. We will consider the problem from the perspectives of stationary control, which involves designing a control policy to be applied over time based on the current state of the network, with the decision procedure itself being time independent. {The objective of a control policy is to optimally reduce the total steady-state probability mass of the undesirable states (phenotypes, which is equivalent to optimally increasing the total steady-state mass of the desirable states. Based on this criterion we compare several proposed network inference procedures. We will see that inference procedure psi may perform poorer than inference procedure xi relative to inferring the full network structure but perform better than xi relative to controllability. Hence, when one is aiming at a specific application, it may be wise to use an objective-based measure of inference validity.

  2. Automated large-scale control of gene regulatory networks.

    Science.gov (United States)

    Tan, Mehmet; Alhajj, Reda; Polat, Faruk

    2010-04-01

    Controlling gene regulatory networks (GRNs) is an important and hard problem. As it is the case in all control problems, the curse of dimensionality is the main issue in real applications. It is possible that hundreds of genes may regulate one biological activity in an organism; this implies a huge state space, even in the case of Boolean models. This is also evident in the literature that shows that only models of small portions of the genome could be used in control applications. In this paper, we empower our framework for controlling GRNs by eliminating the need for expert knowledge to specify some crucial threshold that is necessary for producing effective results. Our framework is characterized by applying the factored Markov decision problem (FMDP) method to the control problem of GRNs. The FMDP is a suitable framework for large state spaces as it represents the probability distribution of state transitions using compact models so that more space and time efficient algorithms could be devised for solving control problems. We successfully mapped the GRN control problem to an FMDP and propose a model reduction algorithm that helps find approximate solutions for large networks by using existing FMDP solvers. The test results reported in this paper demonstrate the efficiency and effectiveness of the proposed approach.

  3. SIFT - Design and analysis of a fault-tolerant computer for aircraft control. [Software Implemented Fault Tolerant systems

    Science.gov (United States)

    Wensley, J. H.; Lamport, L.; Goldberg, J.; Green, M. W.; Levitt, K. N.; Melliar-Smith, P. M.; Shostak, R. E.; Weinstock, C. B.

    1978-01-01

    SIFT (Software Implemented Fault Tolerance) is an ultrareliable computer for critical aircraft control applications that achieves fault tolerance by the replication of tasks among processing units. The main processing units are off-the-shelf minicomputers, with standard microcomputers serving as the interface to the I/O system. Fault isolation is achieved by using a specially designed redundant bus system to interconnect the processing units. Error detection and analysis and system reconfiguration are performed by software. Iterative tasks are redundantly executed, and the results of each iteration are voted upon before being used. Thus, any single failure in a processing unit or bus can be tolerated with triplication of tasks, and subsequent failures can be tolerated after reconfiguration. Independent execution by separate processors means that the processors need only be loosely synchronized, and a novel fault-tolerant synchronization method is described.

  4. Regulatory systems for prevention and control of rabies, Japan.

    Science.gov (United States)

    Takahashi-Omoe, Hiromi; Omoe, Katsuhiko; Okabe, Nobuhiko

    2008-09-01

    Japan is one of the few rabies-free countries. Although 3 imported cases of human rabies were seen in 1970 and 2006, no other cases have been reported for approximately 50 years. The elimination of rabies in Japan is attributed to not only its geographic isolation but also to effective prevention and control measures, such as registration and vaccination of domestic dogs, required quarantine of susceptible imported animals, and national plans of action based on scientific research. Countermeasures against rabies have been upgraded; an improved management system for domestic dogs under the amended Enforcement Regulations of the Rabies Prevention Law has been in effect since April 2007. The latest regulatory systems for preventing and controlling rabies provide an effective model for elimination of the disease worldwide.

  5. An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice

    Directory of Open Access Journals (Sweden)

    Jia Yulin

    2007-06-01

    Full Text Available Abstract Background Plants respond to low temperature through an intricately coordinated transcriptional network. The CBF/DREB-regulated network of genes has been shown to play a prominent role in freeze-tolerance of Arabidopsis through the process of cold acclimation (CA. Recent evidence also showed that the CBF/DREB regulon is not unique to CA but evolutionarily conserved between chilling-insensitive (temperate and chilling-sensitive (warm-season plants. In this study, the wide contrast in chilling sensitivity between indica and japonica rice was used as model to identify other regulatory clusters by integrative analysis of promoter architecture (ab initio and gene expression profiles. Results Transcriptome analysis in chilling tolerant japonica rice identified a subset of 121 'early response' genes that were upregulated during the initial 24 hours at 10°C. Among this group were four transcription factors including ROS-bZIP1 and another larger sub-group with a common feature of having as1/ocs-like elements in their promoters. Cold-induction of ROS-bZIP1 preceded the induction of as1/ocs-like element-containing genes and they were also induced by exogenous H2O2 at ambient temperature. Coordinated expression patterns and similar promoter architectures among the 'early response' genes suggest that they belong to a potential regulon (ROS-bZIP – as1/ocs regulatory module that responds to elevated levels of ROS during chilling stress. Cultivar-specific expression signatures of the candidate genes indicate a positive correlation between the activity of the putative regulon and genotypic variation in chilling tolerance. Conclusion A hypothetical model of an ROS-mediated regulon (ROS-bZIP – as1/ocs triggered by chilling stress was assembled in rice. Based on the current results, it appears that this regulon is independent of ABA and CBF/DREB, and that its activation has an important contribution in configuring the rapid responses of rice seedlings

  6. Controllability analysis of transcriptional regulatory networks reveals circular control patterns among transcription factors.

    Science.gov (United States)

    Österlund, Tobias; Bordel, Sergio; Nielsen, Jens

    2015-05-01

    Transcriptional regulation is the most committed type of regulation in living cells where transcription factors (TFs) control the expression of their target genes and TF expression is controlled by other TFs forming complex transcriptional regulatory networks that can be highly interconnected. Here we analyze the topology and organization of nine transcriptional regulatory networks for E. coli, yeast, mouse and human, and we evaluate how the structure of these networks influences two of their key properties, namely controllability and stability. We calculate the controllability for each network as a measure of the organization and interconnectivity of the network. We find that the number of driver nodes nD needed to control the whole network is 64% of the TFs in the E. coli transcriptional regulatory network in contrast to only 17% for the yeast network, 4% for the mouse network and 8% for the human network. The high controllability (low number of drivers needed to control the system) in yeast, mouse and human is due to the presence of internal loops in their regulatory networks where the TFs regulate each other in a circular fashion. We refer to these internal loops as circular control motifs (CCM). The E. coli transcriptional regulatory network, which does not have any CCMs, shows a hierarchical structure of the transcriptional regulatory network in contrast to the eukaryal networks. The presence of CCMs also has influence on the stability of these networks, as the presence of cycles can be associated with potential unstable steady-states where even small changes in binding affinities can cause dramatic rearrangements of the state of the network.

  7. Fault tolerant vector control of induction motor drive

    Science.gov (United States)

    Odnokopylov, G.; Bragin, A.

    2014-10-01

    For electric composed of technical objects hazardous industries, such as nuclear, military, chemical, etc. an urgent task is to increase their resiliency and survivability. The construction principle of vector control system fault-tolerant asynchronous electric. Displaying recovery efficiency three-phase induction motor drive in emergency mode using two-phase vector control system. The process of formation of a simulation model of the asynchronous electric unbalance in emergency mode. When modeling used coordinate transformation, providing emergency operation electric unbalance work. The results of modeling transient phase loss motor stator. During a power failure phase induction motor cannot save circular rotating field in the air gap of the motor and ensure the restoration of its efficiency at rated torque and speed.

  8. Sensor Fault and Delay Tolerant Control for Networked Control Systems Subject to External Disturbances.

    Science.gov (United States)

    Han, Shi-Yuan; Chen, Yue-Hui; Tang, Gong-You

    2017-03-28

    In this paper, the problem of sensor fault and delay tolerant control problem for a class of networked control systems under external disturbances is investigated. More precisely, the dynamic characteristics of the external disturbance and sensor fault are described as the output of exogenous systems first. The original sensor fault and delay tolerant control problem is reformulated as an equivalence problem with designed available system output and reformed performance index. The feedforward and feedback sensor fault tolerant controller (FFSFTC) can be obtained by utilizing the solutions of Riccati matrix equation and Stein matrix equation. Based on the designed fault diagnoser, the proposed FFSFTC is further reconstructed to compensate for the sensor fault and delayed measurement effects. Finally, numerical examples are provided to illustrate the effectiveness of our proposed FFSFTC with different cases with various types of sensor faults, measurement delays and external disturbances.

  9. Control of experimental inflammatory bowel disease by regulatory T cells.

    Science.gov (United States)

    Asseman, C; Fowler, S; Powrie, F

    2000-10-01

    A helper T cell type 1-mediated colitis driven by enteric bacteria develops in severe combined immunodeficient mice after transfer of CD45RB(high)CD4(+) T cells. Development of disease can be prevented by cotransfer of the reciprocal CD45RB(low) subset. Analysis of the mechanism of immune suppression transferred by CD45RB(low)CD4(+) cells revealed essential roles for both IL-10 and TGF-beta. These data indicate that a functionally specialized population of regulatory T (Treg) cells exists in normal mice and that these can prevent the development of pathogenic responses toward commensal bacteria. The role of Treg cells in the control of the immune response is discussed.

  10. Active Disturbance Rejection Approach for Robust Fault-Tolerant Control via Observer Assisted Sliding Mode Control

    Directory of Open Access Journals (Sweden)

    John Cortés-Romero

    2013-01-01

    Full Text Available This work proposes an active disturbance rejection approach for the establishment of a sliding mode control strategy in fault-tolerant operations. The core of the proposed active disturbance rejection assistance is a Generalized Proportional Integral (GPI observer which is in charge of the active estimation of lumped nonlinear endogenous and exogenous disturbance inputs related to the creation of local sliding regimes with limited control authority. Possibilities are explored for the GPI observer assisted sliding mode control in fault-tolerant schemes. Convincing improvements are presented with respect to classical sliding mode control strategies. As a collateral advantage, the observer-based control architecture offers the possibility of chattering reduction given that a significant part of the control signal is of the continuous type. The case study considers a classical DC motor control affected by actuator faults, parametric failures, and perturbations. Experimental results and comparisons with other established sliding mode controller design methodologies, which validate the proposed approach, are provided.

  11. Plant tolerance: A unique approach to control hemipteran pests

    Science.gov (United States)

    Plant tolerance to insect pests has been indicated to be a unique category of resistance, however, very little information is available on the mechanism of tolerance against insect pests. Tolerance is distinctive in terms of the plant’s ability to withstand or recover from herbivore injury through g...

  12. Regulatory T cells and human myeloid dendritic cells promote tolerance via programmed death ligand-1.

    Directory of Open Access Journals (Sweden)

    Shoba Amarnath

    2010-02-01

    Full Text Available Immunotherapy using regulatory T cells (Treg has been proposed, yet cellular and molecular mechanisms of human Tregs remain incompletely characterized. Here, we demonstrate that human Tregs promote the generation of myeloid dendritic cells (DC with reduced capacity to stimulate effector T cell responses. In a model of xenogeneic graft-versus-host disease (GVHD, allogeneic human DC conditioned with Tregs suppressed human T cell activation and completely abrogated posttransplant lethality. Tregs induced programmed death ligand-1 (PD-L1 expression on Treg-conditioned DC; subsequently, Treg-conditioned DC induced PD-L1 expression in vivo on effector T cells. PD-L1 blockade reversed Treg-conditioned DC function in vitro and in vivo, thereby demonstrating that human Tregs can promote immune suppression via DC modulation through PD-L1 up-regulation. This identification of a human Treg downstream cellular effector (DC and molecular mechanism (PD-L1 will facilitate the rational design of clinical trials to modulate alloreactivity.

  13. Guaranteed Cost Fault-Tolerant Control for Networked Control Systems with Sensor Faults

    OpenAIRE

    Qixin Zhu; Kaihong Lu; Guangming Xie; Yonghong Zhu

    2015-01-01

    For the large scale and complicated structure of networked control systems, time-varying sensor faults could inevitably occur when the system works in a poor environment. Guaranteed cost fault-tolerant controller for the new networked control systems with time-varying sensor faults is designed in this paper. Based on time delay of the network transmission environment, the networked control systems with sensor faults are modeled as a discrete-time system with uncertain parameters. And the mode...

  14. Regulatory control of nuclear safety in Finland. Annual report 1999

    Energy Technology Data Exchange (ETDEWEB)

    Tossavainen, K. [ed.

    2000-06-01

    This report concerns the regulatory control of nuclear energy in Finland in 1999. Its submission to the Ministry of Trade and Industry by the Finnish Radiation and Nuclear Safety Authority (STUK) is stipulated in section 121 of the Nuclear Energy Decree. STUK's regulatory work was focused on the operation of the Finnish nuclear power plants as well as on nuclear waste management and safeguards of nuclear materials. The operation of the Finnish nuclear power plants was in compliance with the conditions set out in their operating licences and with current regulations, with the exception of some inadvertent deviations from the Technical Specifications. No plant events endangering the safe use of nuclear energy occurred. The individual doses of all nuclear power plant workers remained below the dose threshold. The collective dose of the workers was low, compared internationally, and did not exceed STUK's guidelines at either nuclear power plant. The radioactive releases were minor and the dose calculated on their basis for the most exposed individual in the vicinity of the plant was well below the limit established in a decision of the Council of State at both Loviisa and Olkiluoto nuclear power plants. STUK issued statements to the Ministry of Trade and Industry about the environmental impact assessment programme reports on the possible nuclear power plant projects at Olkiluoto and Loviisa and about the continued operation of the research reactor in Otaniemi, Espoo. A Y2k-related safety assessment of the Finnish nuclear power plants was completed in December. In nuclear waste management STUK's regulatory work was focused on spent fuel storage and final disposal plans as well as on the treatment, storage and final disposal of reactor waste. No events occurred in nuclear waste management that would have endangered safety. A statement was issued to the Ministry of Trade and Industry about an environmental impact assessment report on a proposed final

  15. Guaranteed Cost Fault-Tolerant Control for Networked Control Systems with Sensor Faults

    Directory of Open Access Journals (Sweden)

    Qixin Zhu

    2015-01-01

    Full Text Available For the large scale and complicated structure of networked control systems, time-varying sensor faults could inevitably occur when the system works in a poor environment. Guaranteed cost fault-tolerant controller for the new networked control systems with time-varying sensor faults is designed in this paper. Based on time delay of the network transmission environment, the networked control systems with sensor faults are modeled as a discrete-time system with uncertain parameters. And the model of networked control systems is related to the boundary values of the sensor faults. Moreover, using Lyapunov stability theory and linear matrix inequalities (LMI approach, the guaranteed cost fault-tolerant controller is verified to render such networked control systems asymptotically stable. Finally, simulations are included to demonstrate the theoretical results.

  16. Fault tolerant control of multivariable processes using auto-tuning PID controller.

    Science.gov (United States)

    Yu, Ding-Li; Chang, T K; Yu, Ding-Wen

    2005-02-01

    Fault tolerant control of dynamic processes is investigated in this paper using an auto-tuning PID controller. A fault tolerant control scheme is proposed composing an auto-tuning PID controller based on an adaptive neural network model. The model is trained online using the extended Kalman filter (EKF) algorithm to learn system post-fault dynamics. Based on this model, the PID controller adjusts its parameters to compensate the effects of the faults, so that the control performance is recovered from degradation. The auto-tuning algorithm for the PID controller is derived with the Lyapunov method and therefore, the model predicted tracking error is guaranteed to converge asymptotically. The method is applied to a simulated two-input two-output continuous stirred tank reactor (CSTR) with various faults, which demonstrate the applicability of the developed scheme to industrial processes.

  17. Drought tolerance in Arabidopsis is controlled by the OCP3 disease resistance regulator.

    Science.gov (United States)

    Ramírez, Vicente; Coego, Alberto; López, Ana; Agorio, Astrid; Flors, Víctor; Vera, Pablo

    2009-05-01

    Water scarcity and corresponding abiotic drought stress is one of the most important factors limiting plant performance and yield. In addition, plant productivity is severely compromised worldwide by infection with microbial pathogens. Two of the most prominent pathways responsible for drought tolerance and disease resistance to fungal pathogens in Arabidopsis are those controlled by the phytohormones abscisic acid (ABA) and the oxylipin methyl jasmonate (MeJA), respectively. Here, we report on the functional characterization of OCP3, a transcriptional regulator from the homeodomain (HD) family. The Arabidopsis loss-of-function ocp3 mutant exhibits both drought resistance and enhanced disease resistance to necrotrophic fungal pathogens. Double-mutant analysis revealed that these two resistance phenotypes have different genetic requirements. Whereas drought tolerance in ocp3 is ABA-dependent but MeJA-independent, the opposite holds true for the enhanced disease resistance characteristics. These observations lead us to propose a regulatory role of OCP3 in the adaptive responses to these two stresses, functioning as a modulator of independent and specific aspects of the ABA- and MeJA-mediated signal transduction pathways.

  18. Rice Tolerance to Saflufenacil in Clomazone Weed Control Program

    Directory of Open Access Journals (Sweden)

    E. R. Camargo

    2011-01-01

    Full Text Available This research was conducted to evaluate (1 rice tolerance to saflufenacil applied preemergence (PRE and postemergence (POST and (2 the combination of saflufenacil and clomazone in light-textured soils. No injury from PRE application of saflufenacil was observed in 2009, and minimal injury for saflufenacil alone was observed in 2010. Injury as high as 68% was observed with combinations of 505 g ha−1 of clomazone applied PRE and 50 g ha−1 of saflufenacil applied POST in 2009 early evaluations. No interaction between clomazone and POST saflufenacil rates was observed in 2010. Rice injury intensified with increasing rates of saflufenacil applied POST. However, rice recovered with time for herbicide treatments applied PRE and POST in both years. Consequently, rice yield was not affected by any of the saflufenacil rates applied either PRE or POST in a clomazone weed control program.

  19. Research on fault-tolerant control of networked control systems based on information scheduling

    Institute of Scientific and Technical Information of China (English)

    Huo Zhihong; Zhang Zhixue; Fang Huajing

    2008-01-01

    A kind of networked control system is studied; the networked control system with noise disturbance is modeled based on information scheduling and control co-design.Augmented state matrix analysis method is introduced,and robust fault-tolerant control problem of networked control systems with noise disturbance under actuator failures is studied.The parametric expression of the controller under actuator failures is given.Furthermore,the result is analyzed by simulation tests,which not only satisfies the networked control systems stability,but also decreases the data information number in network channel and makes full use of the network resources.

  20. Prognostics Enhancemend Fault-Tolerant Control with an Application to a Hovercraft Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Fault-Tolerant Control (FTC) is an emerging area of engineering and scientific research that integrates prognostics, health management concepts and intelligent...

  1. MATERNAL-FETAL TOLERANCE AS A MANIFESTATION OF REGULATORY CONTINUUM AND PLASTICITY OF THEIR IMMUNE SYSTEMS (in memory of I.P. Ashmarin

    Directory of Open Access Journals (Sweden)

    E. P. Kharchenko

    2011-01-01

    Full Text Available Abstract. Relations of immune tolerance between mother and fetus are considered in view of a common regulatory continuum which is formed under the influence of a growing fetus. To explain such an immune tolerance, a notion of immune system plasticity is introduced, as an analogue to the nervous system plasticity, which develops in response to a continuum of changes occurring in fetal and maternal organisms during the nine months of pregnancy. The immune system plasticity in females is supposed to be among possible factors causing collisions upon conception and in the course of pregnancy. (Med. Immunol., 2011, vol. 13, N 2-3, pp 121-132

  2. Aircraft Attitude Distributed Fault-tolerant Control Based on Dynamic Actuator

    National Research Council Canada - National Science Library

    Zhou Hong-Cheng; Wang Dao-Bo

    2014-01-01

      For attitude control system, based on decentralized fault-tolerant control framework, actuators damage and stuck fault detection and identification unit are designed for the flight control system...

  3. Design of passive fault-tolerant controllers of a quadrotor based on sliding mode theory

    National Research Council Canada - National Science Library

    Abdel-Razzak Merheb; Hassan Noura; François Bateman

    2015-01-01

    .... In this configuration, faults are tolerated in the fast inner loop controlling the velocity system. Tuning the controllers to find the optimal values of the sliding mode controller gains is made using the ecological systems algorithm (ESA...

  4. Plant Tolerance: A Unique Approach to Control Hemipteran Pests

    Science.gov (United States)

    Koch, Kyle G.; Chapman, Kaitlin; Louis, Joe; Heng-Moss, Tiffany; Sarath, Gautam

    2016-01-01

    Plant tolerance to insect pests has been indicated to be a unique category of resistance, however, very little information is available on the mechanism of tolerance against insect pests. Tolerance is distinctive in terms of the plant’s ability to withstand or recover from herbivore injury through growth and compensatory physiological processes. Because plant tolerance involves plant compensatory characteristics, the plant is able to harbor large numbers of herbivores without interfering with the insect pest’s physiology or behavior. Some studies have observed that tolerant plants can compensate photosynthetically by avoiding feedback inhibition and impaired electron flow through photosystem II that occurs as a result of insect feeding. Similarly, the up-regulation of peroxidases and other oxidative enzymes during insect feeding, in conjunction with elevated levels of phytohormones can play an important role in providing plant tolerance to insect pests. Hemipteran insects comprise some of the most economically important plant pests (e.g., aphids, whiteflies), due to their ability to achieve high population growth and their potential to transmit plant viruses. In this review, results from studies on plant tolerance to hemipterans are summarized, and potential models to understand tolerance are presented. PMID:27679643

  5. Active Disturbance Rejection Approach for Robust Fault-Tolerant Control via Observer Assisted Sliding Mode Control

    OpenAIRE

    John Cortés-Romero; Harvey Rojas-Cubides; Horacio Coral-Enriquez; Hebertt Sira-Ramírez; Alberto Luviano-Juárez

    2013-01-01

    This work proposes an active disturbance rejection approach for the establishment of a sliding mode control strategy in fault-tolerant operations. The core of the proposed active disturbance rejection assistance is a Generalized Proportional Integral (GPI) observer which is in charge of the active estimation of lumped nonlinear endogenous and exogenous disturbance inputs related to the creation of local sliding regimes with limited control authority. Possibilities are explored for the GPI obs...

  6. Fault-Tolerant Control for Networked Control Systems with Limited Information in Case of Actuator Fault

    OpenAIRE

    Wang Yan-feng; Wang Pei-liang; Li Zu-xin; Chen Hui-ying

    2015-01-01

    This paper is concerned with the problem of designing a fault-tolerant controller for uncertain discrete-time networked control systems against actuator possible fault. The step difference between the running step k and the time stamp of the used plant state is modeled as a finite state Markov chain of which the transition probabilities matrix information is limited. By introducing actuator fault indicator matrix, the closed-loop system model is obtained by means of state augmentation techniq...

  7. Regulatory roles of 24-epibrassinolide in tolerance of Acacia gerrardii Benth to salt stress.

    Science.gov (United States)

    Abd Allah, Elsayed Fathi; Alqarawi, A A; Hashem, Abeer; Wirth, Stephan; Egamberdieva, Dilfuza

    2017-07-11

    This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the negative effect considerably. NaCl reduced chlorophyll content but this was significantly ameliorated by the application of EBL. EBL reduced significantly NaCl-induced oxidative stress hence protect membranes and also improved the relative water content significantly by 6.6% as compared with control. Nitrate reductase activity declined after NaCl treatment but EBL application sustained its activity under normal and stressed conditions. Exogenous application of EBL significantly improved the activity of superoxide dismutase, catalase and the enzymes of the ascorbate-glutathione pathway thereby protecting the photosynthetic electron transport chain and other metabolic processes in A. gerrardii from NaCl-induced oxidative stress.

  8. Adolescent threat-related interpretive bias and its modification: the moderating role of regulatory control

    NARCIS (Netherlands)

    Salemink, E.; Wiers, R.W.

    2012-01-01

    Dual process models describe psychopathology as the consequence of an imbalance between a fast, impulsive system and a regulatory control system and have recently been applied to anxiety disorders. The aim of the current study was to specifically examine the role of a regulatory control system in re

  9. Notch1 regulated autophagy controls survival and suppressor activity of activated murine T-regulatory cells

    Science.gov (United States)

    Marcel, Nimi; Sarin, Apurva

    2016-01-01

    Cell survival is one of several processes regulated by the Notch pathway in mammalian cells. Here we report functional outcomes of non-nuclear Notch signaling to activate autophagy, a conserved cellular response to nutrient stress, regulating survival in murine natural T-regulatory cells (Tregs), an immune subset controlling tolerance and inflammation. Induction of autophagy required ligand-dependent, Notch intracellular domain (NIC) activity, which controlled mitochondrial organization and survival of activated Tregs. Consistently, NIC immune-precipitated Beclin and Atg14, constituents of the autophagy initiation complex. Further, ectopic expression of an effector of autophagy (Atg3) or recombinant NIC tagged to a nuclear export signal (NIC-NES), restored autophagy and suppressor function in Notch1-/- Tregs. Furthermore, Notch1 deficiency in the Treg lineage resulted in immune hyperactivity, implicating Notch activity in Treg homeostasis. Notch1 integration with autophagy, revealed in these experiments, holds implications for Notch regulated cell-fate decisions governing differentiation. DOI: http://dx.doi.org/10.7554/eLife.14023.001 PMID:27267497

  10. Fault detection and fault-tolerant control for nonlinear systems

    CERN Document Server

    Li, Linlin

    2016-01-01

    Linlin Li addresses the analysis and design issues of observer-based FD and FTC for nonlinear systems. The author analyses the existence conditions for the nonlinear observer-based FD systems to gain a deeper insight into the construction of FD systems. Aided by the T-S fuzzy technique, she recommends different design schemes, among them the L_inf/L_2 type of FD systems. The derived FD and FTC approaches are verified by two benchmark processes. Contents Overview of FD and FTC Technology Configuration of Nonlinear Observer-Based FD Systems Design of L2 nonlinear Observer-Based FD Systems Design of Weighted Fuzzy Observer-Based FD Systems FTC Configurations for Nonlinear Systems< Application to Benchmark Processes Target Groups Researchers and students in the field of engineering with a focus on fault diagnosis and fault-tolerant control fields The Author Dr. Linlin Li completed her dissertation under the supervision of Prof. Steven X. Ding at the Faculty of Engineering, University of Duisburg-Essen, Germany...

  11. Donor-specific Regulatory T Cells Acquired from Tolerant Mice Bearing Cardiac allograft Promote Mixed Chimerism and Prolong Intestinal Allograft Survival

    Directory of Open Access Journals (Sweden)

    Xiaofei Shen

    2016-11-01

    Full Text Available The induction of donor-specific transplant tolerance has always been a central problem for small bowel transplantation, which is thought to be the best therapy for end-stage bowel failure. With the development of new tolerance-inducing strategies, mixed chimerism induced by co-stimulation blockade has become most potent for tolerance of allografts such as skin, kidney and heart. However, a lack of clinically available co-stimulation blockers has hindered efficient application in humans. Furthermore, unlike those for other types of solid organ transplantation, strategies to induce robust mixed chimerism for intestinal allografts have not been fully developed. To improve current mixed chimerism induction protocols for future clinical application, we developed a new protocol using donor-specific regulatory T (Treg cells from mice with heart allograft tolerance, clinically available immunosuppressive drugs, and low doses of irradiation. Our results demonstrated that donor-specific Treg cells acquired from tolerant mice after in vitro expansion generate stable chimerism and lead to acceptance of intestinal allograft. Increased intragraft Treg cells and clonal deletion both contribute to the development of small bowel transplantation tolerance.

  12. Fault-Tolerant Control for Networked Control Systems with Limited Information in Case of Actuator Fault

    Directory of Open Access Journals (Sweden)

    Wang Yan-feng

    2015-01-01

    Full Text Available This paper is concerned with the problem of designing a fault-tolerant controller for uncertain discrete-time networked control systems against actuator possible fault. The step difference between the running step k and the time stamp of the used plant state is modeled as a finite state Markov chain of which the transition probabilities matrix information is limited. By introducing actuator fault indicator matrix, the closed-loop system model is obtained by means of state augmentation technique. The sufficient conditions on the stochastic stability of the closed-loop system are given and the fault-tolerant controller is designed by solving a linear matrix inequality. A numerical example is presented to illustrate the effectiveness of the proposed method.

  13. Research on robust fault-tolerant control for networked control system with packet dropout

    Institute of Scientific and Technical Information of China (English)

    Huo Zhihong; Fang Huajing

    2007-01-01

    A kind of networked control system with network-induced delay and packet dropout, modeled on asynchronous dynamical systems was tested, and the integrity design of the networked control system with sensors failures and actuators failures was analyzed using hybrid systems technique based on the robust fault-tolerant control theory. The parametric expression of controller is given based on the feasible solution of linear matrix inequality. The simulation results are provided on the basis of detailed theoretical analysis, which further demonstrate the validity of the proposed schema.

  14. Diagnosis and Fault-Tolerant Control for Thruster-Assisted Position Mooring System

    DEFF Research Database (Denmark)

    Nguyen, Trong Dong; Blanke, Mogens; Sørensen, Asgeir

    2007-01-01

    Development of fault-tolerant control systems is crucial to maintain safe operation of o®shore installations. The objective of this paper is to develop a fault- tolerant control for thruster-assisted position mooring (PM) system with faults occurring in the mooring lines. Faults in line...

  15. Fault Tolerant Flight Control Using Sliding Modes and Subspace Identification-Based Predictive Control

    KAUST Repository

    Siddiqui, Bilal A.

    2016-07-26

    In this work, a cascade structure of a time-scale separated integral sliding mode and model predictive control is proposed as a viable alternative for fault-tolerant control. A multi-variable sliding mode control law is designed as the inner loop of the flight control system. Subspace identification is carried out on the aircraft in closed loop. The identified plant is then used for model predictive controllers in the outer loop. The overall control law demonstrates improved robustness to measurement noise, modeling uncertainties, multiple faults and severe wind turbulence and gusts. In addition, the flight control system employs filters and dead-zone nonlinear elements to reduce chattering and improve handling quality. Simulation results demonstrate the efficiency of the proposed controller using conventional fighter aircraft without control redundancy.

  16. B7 / CD28 in central tolerance: costimulation promotes maturation of regulatory T cell precursors and prevents their clonal deletion

    Directory of Open Access Journals (Sweden)

    Maria eHinterberger

    2011-07-01

    Full Text Available According to the two-step model, the intrathymic generation of CD4+ regulatory T (Treg cells segregates into a first, T cell receptor (TCR-driven phase and a second, cytokine dependent phase. The initial TCR stimulus gives rise to a CD25+Foxp3– developmental intermediate. These precursors subsequently require cytokine signaling to establish the mature CD25+Foxp3+ Treg cell phenotype. In addition, costimulation via CD28 / B7 (CD80/86 axis is important for the generation of a Treg cell repertoire of normal size. Recent data suggest that CD28 or B7 deficient mice lack CD25+Foxp3– Treg cell progenitors. However, these data leave open whether costimulation is also required at subsequent stages of Treg differentiation. Also, the fate of presumptive Treg cells carrying a permissive TCR specificity in the absence of costimulation remains to be established. Here, we have used a previously described TCR transgenic model of agonist-driven Treg differentiation in order to address these issues. Intrathymic adoptive transfer of Treg precursors indicated that costimulation is dispensable once the intermediate CD25+Foxp3– stage has been reached. Furthermore, lack of costimulation led to the physical loss of presumptive Treg cells rather than their escape from central tolerance and differentiation into the conventional CD4+ T cell lineage. Our findings suggest that CD28 signaling does not primarily operate through enhancing the TCR signal strength in order to pass the threshold intensity required to initiate Treg cell specification. Instead, costimulation seems to deliver unique and qualitatively distinct signals that coordinately foster the developmental progression of Treg precursors and prevent their negative selection.

  17. Robust Fault-Tolerant Tracking Control for Nonlinear Networked Control System: Asynchronous Switched Polytopic Approach

    Directory of Open Access Journals (Sweden)

    Chaoyang Dong

    2015-01-01

    Full Text Available This paper is concerned with the robust fault-tolerant tracking control problem for networked control system (NCS. Firstly, considering the locally overlapped switching law widely existed in engineering applications, the NCS is modeled as a locally overlapped switched polytopic system to reduce designing conservatism and solving complexity. Then, switched parameter dependent fault-tolerant tracking controllers are constructed to deal with the asynchronous switching phenomenon caused by the updating delays of the switching signals and weighted coefficients. Additionally, the global uniform asymptotic stability in the mean (GUAS-M and desired weighted l2 performance are guaranteed by combining the switched parameter dependent Lyapunov functional method with the average dwell time (ADT method, and the feasible conditions for the fault-tolerant tracking controllers are obtained in the form of linear matrix inequalities (LMIs. Finally, the performance of the proposed approach is verified on a highly maneuverable technology (HiMAT vehicle’s tracking control problem. Simulation results show the effectiveness of the proposed method.

  18. Critical role of intestinal interleukin-4 modulating regulatory T cells for desensitization, tolerance, and inflammation of food allergy

    Science.gov (United States)

    Fujimura, Yoko; Takeyama, Jun; Hiraide, Erika; Kikuchi, Akira; Murakami, Hitoshi; Hosono, Akira; Nochi, Tomonori; Wakatsuki, Yoshio; Shimojo, Naoki; Kaminogawa, Shuichi; Sato, Ryuichiro; Kiyono, Hiroshi; Hachimura, Satoshi

    2017-01-01

    Background and objective The mechanism inducing either inflammation or tolerance to orally administered food allergens remains unclear. To investigate this we analyzed mouse models of food allergy (OVA23-3) and tolerance (DO11.10 [D10]), both of which express ovalbumin (OVA)-specific T-cell receptors. Methods OVA23-3, recombination activating gene (RAG)-2-deficient OVA23-3 (R23-3), D10, and RAG-2-deficient D10 (RD10) mice consumed a diet containing egg white (EW diet) for 2–28 days. Interleukin (IL)-4 production by CD4+ T cells was measured as a causative factor of enteropathy, and anti-IL-4 antibody was used to reveal the role of Foxp3+ OVA-specific Tregs (aiTreg) in this process. Results Unlike OVA23-3 and R23-3 mice, D10 and RD10 mice did not develop enteropathy and weight loss on the EW diet. On days 7–10, in EW-fed D10 and RD10 mice, splenic CD4+ T cells produced significantly more IL-4 than did those in the mesenteric lymph nodes (MLNs); this is in contrast to the excessive IL-4 response in the MLNs of EW-fed OVA23-3 and R23-3 mice. EW-fed R23-3 mice had few aiTregs, whereas EW-fed RD10 mice had them in both tissues. Intravenous injections of anti-IL-4 antibody recovered the percentage of aiTregs in the MLNs of R23-3 mice. On day 28, in EW-fed OVA23-3 and R23-3 mice, expression of Foxp3 on CD4+ T cells corresponded with recovery from inflammation, but recurrence of weight loss was observed on restarting the EW diet after receiving the control-diet for 1 month. No recurrence developed in D10 mice. Conclusions Excessive IL-4 levels in the MLNs directly inhibited the induction of aiTregs and caused enteropathy. The aiTregs generated in the attenuation of T cell-dependent food allergic enteropathy may function differently than aiTregs induced in a tolerance model. Comparing the two models enables to investigate their aiTreg functions and to clarify differences between inflammation with subsequent desensitization versus tolerance. PMID:28234975

  19. Identification of a second two-component signal transduction system that controls fosfomycin tolerance and glycerol-3-phosphate uptake.

    Science.gov (United States)

    Kurabayashi, Kumiko; Hirakawa, Yuko; Tanimoto, Koichi; Tomita, Haruyoshi; Hirakawa, Hidetada

    2015-03-01

    Particular interest in fosfomycin has resurfaced because it is a highly beneficial antibiotic for the treatment of refractory infectious diseases caused by pathogens that are resistant to other commonly used antibiotics. The biological cost to cells of resistance to fosfomycin because of chromosomal mutation is high. We previously found that a bacterial two-component system, CpxAR, induces fosfomycin tolerance in enterohemorrhagic Escherichia coli (EHEC) O157:H7. This mechanism does not rely on irreversible genetic modification and allows EHEC to relieve the fitness burden that results from fosfomycin resistance in the absence of fosfomycin. Here we show that another two-component system, TorSRT, which was originally characterized as a regulatory system for anaerobic respiration utilizing trimethylamine-N-oxide (TMAO), also induces fosfomycin tolerance. Activation of the Tor regulatory pathway by overexpression of torR, which encodes the response regulator, or addition of TMAO increased fosfomycin tolerance in EHEC. We also show that phosphorylated TorR directly represses the expression of glpT, a gene that encodes a symporter of fosfomycin and glycerol-3-phosphate, and activation of the TorR protein results in the reduced uptake of fosfomycin by cells. However, cells in which the Tor pathway was activated had an impaired growth phenotype when cultured with glycerol-3-phosphate as a carbon substrate. These observations suggest that the TorSRT pathway is the second two-component system to reversibly control fosfomycin tolerance and glycerol-3-phosphate uptake in EHEC, and this may be beneficial for bacteria by alleviating the biological cost. We expect that this mechanism could be a potential target to enhance the utility of fosfomycin as chemotherapy against multidrug-resistant pathogens. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  20. The regulatory pyramid meets the food pyramid: can regulatory theory improve controls on television food advertising to Australian children?

    Science.gov (United States)

    Reeve, Belinda

    2011-09-01

    This article examines whether responsive regulation has potential to improve the regulatory framework which controls free-to-air television advertising to children, so that the regulatory scheme can be used more effectively as a tool for obesity prevention. It presents two apparently conflicting arguments, the first being that responsive regulation, particularly monitoring and enforcement measures, can be used to refine the regulation of children's food advertising. The second argument is that there are limits to the improvements that responsive regulation can achieve, since it is trying to achieve the wrong goal, namely placing controls on misleading or deceptive advertising techniques rather than diminishing the sheer volume of advertisements to which children are exposed. These two positions reflect a conflict between public health experts and governments regarding the role of industry in chronic disease prevention, as well as a broader debate about how best to regulate industry.

  1. Fault-tolerant control of linear uncertain systems using H∞ robust predictive control

    Institute of Scientific and Technical Information of China (English)

    Chen Xueqin; Geng Yunhai; Zhang Yingchun; Wang Feng

    2008-01-01

    The robust fault-tolerant control problem of linear uncertain systems is studied. It is shown that a solution for this problem can be obtained from a H∞ robust predictive controller (RMPC) by the method of linear matrix inequality (LMI). This approach has the advantages of both H∞ control and MPC: the robustness and ability to handle constraints explicitly. The robust closed-loop stability of the linear uncertain system with input and output constraints is proven under an actuator and sensor faults condition. Finally, satisfactory results of simulation experiments verify the validity of this algorithm.

  2. Attack-tolerant networked control system: an approach for detection the controller stealthy hijacking attack

    Science.gov (United States)

    Atta Yaseen, Amer; Bayart, Mireille

    2017-01-01

    In this work, a new approach will be introduced as a development for the attack-tolerant scheme in the Networked Control System (NCS). The objective is to be able to detect an attack such as the Stuxnet case where the controller is reprogrammed and hijacked. Besides the ability to detect the stealthy controller hijacking attack, the advantage of this approach is that there is no need for a priori mathematical model of the controller. In order to implement the proposed scheme, a specific detector for the controller hijacking attack is designed. The performance of this scheme is evaluated be connected the detector to NCS with basic security elements such as Data Encryption Standard (DES), Message Digest (MD5), and timestamp. The detector is tested along with networked PI controller under stealthy hijacking attack. The test results of the proposed method show that the hijacked controller can be significantly detected and recovered.

  3. Fault-Tolerant Control of the Road Wheel Subsystem in a Steer-By-Wire System

    Directory of Open Access Journals (Sweden)

    Bing Zheng

    2008-01-01

    Full Text Available This paper describes a fault-tolerant steer-by-wire road wheel control system. With dual motor and dual microcontroller architecture, this system has the capability to tolerate single-point failures without degrading the control system performance. The arbitration bus, mechanical arrangement of motors, and the developed control algorithm allow the system to reconfigure itself automatically in the event of a single-point fault, and assure a smooth reconfiguration process. Both simulation and experimental results illustrate the effectiveness of the proposed fault-tolerant control system.

  4. Active and Passive Fault-Tolerant LPV Control of Wind Turbines

    DEFF Research Database (Denmark)

    Sloth, Christoffer; Esbensen, Thomas; Stoustrup, Jakob

    2010-01-01

    of an incipient fault in the pitch system. We propose the design of an active fault-tolerant controller (AFTC) based on an existing LPV controller design method and extend this method to apply for the design of a passive fault-tolerant controller (PFTC). Both controllers are based on output feedback...... is more difficult to solve, as it involves solving bilinear matrix inequalities (BMIs) instead of linear matrix inequalities (LMIs). Simulation results show the performance of the active faulttolerant control system to be slightly superior to that of the passive fault-tolerant control system.......This paper addresses the design and comparison of active and passive fault-tolerant linear parameter-varying (LPV) controllers for wind turbines. The considered wind turbine plant model is characterized by parameter variations along the nominal operating trajectory and includes a model...

  5. Regulatory Mode Preferences for Autonomy Supporting versus Controlling Instructional Styles

    Science.gov (United States)

    Pierro, Antonio; Presaghi, Fabio; Higgins, Tory E.; Kruglanski, Arie W.

    2009-01-01

    Background: Three studies carried out in educational settings examined determinants of teacher's instructional styles and students' degree of satisfaction with the learning climates created by such styles. Aims: Based upon regulatory mode theory, Studies 1 and 2 tested the hypotheses that teachers' locomotion orientation will be positively…

  6. Fault-tolerant design of local controller for the poloidal field converter control system on ITER

    Energy Technology Data Exchange (ETDEWEB)

    Shen, Jun; Fu, Peng; Gao, Ge; He, Shiying; Huang, Liansheng, E-mail: huangls@ipp.ac.cn; Zhu, Lili; Chen, Xiaojiao

    2016-11-15

    Highlights: • The requirements on the Local Control Cubicles (LCC) for ITER Poloidal Field Converter are analyzed. • Decoupled service-based software architecture is proposed to make control loops on LCC running at varying cycle-time. • Fault detection and recovery methods for the LCC are developed to enhance the system. • The performance of the LCC with or without fault-tolerant feature is tested and compared. - Abstract: The control system for the Poloidal Field (PF) on ITER is a synchronously networked control system, which has several kinds of computational controllers. The Local Control Cubicles (LCC) play a critical role in the networked control system for they are the interface to all input and output signals. Thus, some additional work must be done to guarantee the LCCs proper operation under influence of faults. This paper mainly analyzes the system demands of the LCCs and faults which have been encountered recently. In order to handle these faults, decoupled service-based software architecture has been proposed. Based on this architecture, fault detection and system recovery methods, such as redundancy and rejuvenation, have been incorporated to achieve a fault-tolerant private network with the aid of QNX operating system. Unlike the conventional method, this method requires no additional hardware and can be achieved relatively easily. To demonstrate effectiveness the LCCs have been successfully tested during the recent PF Converter Unit performance tests for ITER.

  7. Adaptive fault-tolerant control of linear systems with actuator saturation and L2-disturbances

    Institute of Scientific and Technical Information of China (English)

    Wei GUAN; Guanghong YANG

    2009-01-01

    This paper studies the problem of designing adaptive fault-tolerant H-infinity controllers for linear timeinvariant systems with actuator saturation. The disturbance tolerance ability of the closed-loop system is measured by an optimal index. The notion of an adaptive H-infinity performance index is proposed to describe the disturbance attenuation performances of closed-loop systems. New methods for designing indirect adaptive fault-tolerant controllers via state feedback are presented for actuator fault compensations. Based on the on-line estimation of eventual faults, the adaptive fault-tolerant controller parameters are updated automatically to compensate for the fault effects on systems. The designs are developed in the framework of the linear matrix inequality (LMI) approach, which can guarantee the disturbance tolerance ability and adaptive H-infinity performances of closed-loop systems in the cases of actuator saturation and actuator failures. An example is given to illustrate the efficiency of the design method.

  8. An Integrated Fault Tolerant Robotic Controller System for High Reliability and Safety

    Science.gov (United States)

    Marzwell, Neville I.; Tso, Kam S.; Hecht, Myron

    1994-01-01

    This paper describes the concepts and features of a fault-tolerant intelligent robotic control system being developed for applications that require high dependability (reliability, availability, and safety). The system consists of two major elements: a fault-tolerant controller and an operator workstation. The fault-tolerant controller uses a strategy which allows for detection and recovery of hardware, operating system, and application software failures.The fault-tolerant controller can be used by itself in a wide variety of applications in industry, process control, and communications. The controller in combination with the operator workstation can be applied to robotic applications such as spaceborne extravehicular activities, hazardous materials handling, inspection and maintenance of high value items (e.g., space vehicles, reactor internals, or aircraft), medicine, and other tasks where a robot system failure poses a significant risk to life or property.

  9. Adolescent threat-related interpretive bias and its modification: the moderating role of regulatory control.

    Science.gov (United States)

    Salemink, Elske; Wiers, Reinout W

    2012-01-01

    Dual process models describe psychopathology as the consequence of an imbalance between a fast, impulsive system and a regulatory control system and have recently been applied to anxiety disorders. The aim of the current study was to specifically examine the role of a regulatory control system in regulating 1) threat-related interpretive bias and 2) the effectiveness of interpretive bias training in adolescents. In total, 67 adolescents participated and followed either a positive Cognitive Bias Modification of Interpretation (CBM-I) training or a placebo-control condition. Results revealed that interpretive bias and the effectiveness of its modification depended on individual differences in regulatory control. That is, low levels of regulatory control in combination with high levels of state anxiety were associated with the strongest threat-related interpretive bias and those individuals benefited the most of the positive interpretation training. The current study provided empirical support for the role of dual processes in adolescent threat-related interpretive bias.

  10. Decentralized Fault Tolerant Control for a Class of Interconnected Nonlinear Systems.

    Science.gov (United States)

    Shao, Shuai; Yang, Hao; Jiang, Bin; Cheng, Shuyao

    2016-11-22

    This paper proposes a decentralized fault tolerant methodology for a class of interconnected nonlinear systems. The key novelty of our proposed method is that fault tolerant control can be achieved without necessarily exchanging the state information between the subsystems and the couplings' effect can be dealt with utilizing the cyclic-small-gain methodology. Simulation results demonstrate effectively the validity of our proposed approach.

  11. SIFT - Multiprocessor architecture for Software Implemented Fault Tolerance flight control and avionics computers

    Science.gov (United States)

    Forman, P.; Moses, K.

    1979-01-01

    A brief description of a SIFT (Software Implemented Fault Tolerance) Flight Control Computer with emphasis on implementation is presented. A multiprocessor system that relies on software-implemented fault detection and reconfiguration algorithms is described. A high level reliability and fault tolerance is achieved by the replication of computing tasks among processing units.

  12. Low-income, minority fathers' control strategies and their children's regulatory skills.

    Science.gov (United States)

    Malin, Jenessa L; Cabrera, Natasha J; Karberg, Elizabeth; Aldoney, Daniela; Rowe, Meredith L

    2014-01-01

    The current study explored the bidirectional association of children's individual characteristics, fathers' control strategies at 24 months, and children's regulatory skills at prekindergarten (pre-K). Using a sample of low-income, minority families with 2-year-olds from the Early Head Start Research and Evaluation Project (n = 71), we assessed the association between child gender and vocabulary skills, fathers' control strategies at 24 months (e.g., regulatory behavior and regulatory language), and children's sustained attention and emotion regulation at prekindergarten. There were three main findings. First, fathers overwhelmingly used commands (e.g., "Do that.") to promote compliance in their 24-month-old children. Second, children's vocabulary skills predicted fathers' regulatory behaviors during a father-child interaction whereas children's gender predicted fathers' regulatory language during an interaction. Third, controlling for maternal supportiveness, fathers' regulatory behaviors at 24 months predicted children's sustained attention at pre-K whereas fathers' regulatory language at 24 months predicted children's emotion regulation at pre-K. Our findings highlight the importance of examining paternal contributions to children's regulatory skills.

  13. Passive Fault Tolerant Control of Piecewise Affine Systems Based on H Infinity Synthesis

    DEFF Research Database (Denmark)

    Gholami, Mehdi; Cocquempot, vincent; Schiøler, Henrik

    2011-01-01

    In this paper we design a passive fault tolerant controller against actuator faults for discretetime piecewise affine (PWA) systems. By using dissipativity theory and H analysis, fault tolerant state feedback controller design is expressed as a set of Linear Matrix Inequalities (LMIs). In the cur......). In the current paper, the PWA system switches not only due to the state but also due to the control input. The method is applied on a large scale livestock ventilation model....

  14. Satellite Laser Ranging Photon-Budget Calculations for a Single Satellite Cornercube Retroreflector: Attitude Control Tolerance

    Science.gov (United States)

    2015-11-01

    UNCLASSIFIED Satellite Laser Ranging Photon-Budget Calculations for a Single Satellite Cornercube Retroreflector: Attitude Control Tolerance Philip C...the SLR station, and the direction of the satellite from the SLR station. The required attitude control tolerance is to within 17◦ of the optimal... attitude control strategy determined in the present work. A pre-launch measurement of the re- flectance (diffraction) pattern of each retroreflector is

  15. Ship Propulsion System as a Benchmark for Fault-Tolerant Control

    OpenAIRE

    Izadi-Zamanabadi, Roozbeh; Blanke, M.

    1998-01-01

    Fault-tolerant control combines fault detection and isolation techniques with supervisory control to achieve autonomous accommodation of faults before they develop into failures. While fault detection and isolation (FDI) methods have matured during the past decade the extension to fault-tolerant control is a fairly new area. The paper presents a ship propulsion system as a benchmark that should be useful as a platform for development of new ideas and comparison of methods. The benchmark has t...

  16. A Fault-tolerant Development Methodology for Industrial Control Systems

    DEFF Research Database (Denmark)

    Izadi-Zamanabadi, Roozbeh; Thybo, C.

    2004-01-01

    and logically sound manner. This paper presents the employe fault-tolerant development methodology and highlights steps, which have been essential for achieving complete and consistent monitoring capabilities. Fault diagnosis for a commercial refrigeration system is treated as a case-study....

  17. Modular Multilevel Converter Control Strategy with Fault Tolerance

    DEFF Research Database (Denmark)

    Teodorescu, Remus; Eni, Emanuel-Petre; Mathe, Laszlo;

    2013-01-01

    The Modular Multilevel Converter (MMC) technology has recently emerged in VSC-HVDC applications where it demonstrated higher efficiency and fault tolerance compared to the classical 2-level topology. Due to the ability of MMC to connect to HV levels, MMC can be also used in transformerless STATCO...

  18. Complex shape product tolerance and accuracy control method for virtual assembly

    Science.gov (United States)

    Ma, Huiping; Jin, Yuanqiang; Zhang, Xiaoguang; Zhou, Hai

    2015-02-01

    The simulation of virtual assembly process for engineering design lacks of accuracy in the software of three-dimension CAD at present. Product modeling technology with tolerance, assembly precision preanalysis technique and precision control method are developed. To solve the problem of lack of precision information transmission in CAD, tolerance mathematical model of Small Displacement Torsor (SDT) is presented, which can bring about technology transfer and establishment of digital control function for geometric elements from the definition, description, specification to the actual inspection and evaluation process. Current tolerance optimization design methods for complex shape product are proposed for optimization of machining technology, effective cost control and assembly quality of the products.

  19. A Fault-Tolerant Multiprocessor for Real-Time Control Applications

    Science.gov (United States)

    Roberts, Thomas E.; Johnson, Barry W.

    1987-10-01

    This paper presents the design, analysis, and experimental evaluation of a fault-tolerant multiprocessor for use in systems requiring real-time, microprocessor-based control. Example applications of the fault-tolerant system are found in robotics, process control, manufacturing, and factory automation. The architecture for the multiprocessor is presented and analyzed for reliability, availability, and safety. A prototype of the fault-tolerant multiprocessor has been constructed, using Intel 8088 processors, and experimentally evaluated in the laboratory. Both hardware and software descriptions of the system are provided, and an example application to the control of an electric wheelchair is presented.

  20. Establishment of photosynthesis is controlled by two distinct regulatory phases.

    Science.gov (United States)

    Dubreuil, Carole; Jin, Xu; Barajas-López, Juan de Dios; Hewitt, Timothy Hewitt; Tanz, Sandra; Dobrenel, Thomas; Schröder, Wolfgang; Hanson, Johannes; Pesquet, Edouard; Grönlund, Andreas; Small, Ian D; Strand, Asa

    2017-06-16

    Chloroplasts develop from undifferentiated proplastids present in meristematic tissue. Chloroplast biogenesis is thus closely connected to leaf development, which restricts our ability to study the process of chloroplast biogenesis per se. As a consequence we know relatively little about the regulatory mechanisms behind the establishment of the photosynthetic reactions and how the activities of the two genomes involved are coordinated during chloroplast development. We developed a single-cell-based experimental system from Arabidopsis with high temporal resolution allowing for investigations of the transition from proplastids to functional chloroplasts. Using this unique cell line we could show that establishment of photosynthesis is dependent on a regulatory mechanism involving two distinct phases. The first phase is triggered by rapid light-induced changes in gene expression and the metabolome. The second phase is dependent on the activation of the chloroplast and generates massive changes in nuclear gene expression required for the transition to photosynthetically functional chloroplasts.. The second phase is also associated with a spatial transition of the chloroplasts from clusters around the nucleus to the final position at the cell cortex. Thus, establishment of photosynthesis is a two-phase process with a clear checkpoint associated with the second regulatory phase allowing coordination of the activities of the nuclear and plastid genomes. {copyright, serif} 2017 American Society of Plant Biologists. All rights reserved.

  1. Sensor-coupled fractal gene regulatory networks for locomotion control of a modular snake robot

    DEFF Research Database (Denmark)

    Zahadat, Payam; Christensen, David Johan; Katebi, Serajeddin

    2013-01-01

    In this paper we study fractal gene regulatory network (FGRN) controllers based on sensory information. The FGRN controllers are evolved to control a snake robot consisting of seven simulated ATRON modules. Each module contains three tilt sensors which represent the direction of gravity in the co......In this paper we study fractal gene regulatory network (FGRN) controllers based on sensory information. The FGRN controllers are evolved to control a snake robot consisting of seven simulated ATRON modules. Each module contains three tilt sensors which represent the direction of gravity...

  2. Development Status of the Rad-Tolerant TTEthernet Controller

    Science.gov (United States)

    Fidi, Christian; van Masar, Ivan

    2016-08-01

    The use of switched networking technologies for aerospace and more recently automotive brings additional advantages for space applications like the increase in performance of the overall avionics of a spacecraft. These networks are characterized by a central device (switch) and a point-to-point structure between switch and terminal devices that eases electrical and logical insulation.However, for a use in highly-reliable or highly-available applications as in launchers or satellites systems, these network technologies need to provide built-in determinism and redundancy to fulfill the tight latency and jitter requirements of the avionics control loops and the respective hardware redundancy. Therefore a state of the art networking technology already provides these features and allows the modularity and scalability to be used for the different space applications and would allow combining the deterministic avionics with the high speed payload network in a spacecraft [1].Introducing the time-triggered principle to Ethernet allows combining the open industry standard IEE802.3 Ethernet currently use in almost all GSE platforms, with full control of latency and jitter of the time-triggered approach. To allow the time-triggered data flow over Ethernet, a network- wide synchronization time-base has to be established to allow deriving all network events on a globally known time which is typically done in software in almost all spacecrafts. The additional synchronization service of Time-triggered Ethernet has been implemented as additional quality of service (QoS) on layer 2 of the ISO/OSI network model and been standardized in the SAE AS6802 [3].Within a launcher, the communication system ensured the data exchanges between avionic functions during all phases of the launcher lifecycle which is composed of three areas: AIT operations, ground phase and flight phase. To ensure the use of a single network for the different phases, the network needs to support features like the

  3. Design of passive fault-tolerant controllers of a quadrotor based on sliding mode theory

    Directory of Open Access Journals (Sweden)

    Merheb Abdel-Razzak

    2015-09-01

    Full Text Available Abstract In this paper, sliding mode control is used to develop two passive fault tolerant controllers for an AscTec Pelican UAV quadrotor. In the first approach, a regular sliding mode controller (SMC augmented with an integrator uses the robustness property of variable structure control to tolerate partial actuator faults. The second approach is a cascaded sliding mode controller with an inner and outer SMC loops. In this configuration, faults are tolerated in the fast inner loop controlling the velocity system. Tuning the controllers to find the optimal values of the sliding mode controller gains is made using the ecological systems algorithm (ESA, a biologically inspired stochastic search algorithm based on the natural equilibrium of animal species. The controllers are tested using SIMULINK in the presence of two different types of actuator faults, partial loss of motor power affecting all the motors at once, and partial loss of motor speed. Results of the quadrotor following a continuous path demonstrated the effectiveness of the controllers, which are able to tolerate a significant number of actuator faults despite the lack of hardware redundancy in the quadrotor system. Tuning the controller using a faulty system improves further its ability to afford more severe faults. Simulation results show that passive schemes reserve their important role in fault tolerant control and are complementary to active techniques

  4. Statin tolerability: In defence of placebo-controlled trials

    OpenAIRE

    Tobert, Jonathan A; Newman, Connie B.

    2015-01-01

    Background Statin intolerance is a barrier to effective lipid-lowering treatment. A significant number of patients stop prescribed statins, or can take only a reduced dose, because of adverse events attributed to the statin, and are then considered statin-intolerant. Methods Examination of differences between statin and placebo in withdrawal rates due to adverse events – a good measure of tolerability – in statin cardiovascular outcome trials in patients with advanced disease and complex medi...

  5. Fault Tolerant Control Using Proportional-Integral-Derivative Controller Tuned by Genetic Algorithm

    Directory of Open Access Journals (Sweden)

    S. Kanthalakshmi

    2011-01-01

    Full Text Available Problem statement: The growing demand for reliability, maintainability and survivability in industrial processes has drawn significant research in fault detection and fault tolerant control domain. A fault is usually defined as an unexpected change in a system, such as component malfunction and variations in operating condition, which tends to degrade the overall system performance. The purpose of fault detection is to detect these malfunctions to take proper action in order to prevent faults from developing into a total system failure. Approach: In this study an effective integrated fault detection and fault tolerant control scheme was developed for a class of LTI system. The scheme was based on a Kalman filter for simultaneous state and fault parameter estimation, statistical decisions for fault detection and activation of controller reconfiguration. Proportional-Integral-Derivative (PID control schemes continue to provide the simplest and yet effective solutions to most of the control engineering applications today. Determination or tuning of the PID parameters continues to be important as these parameters have a great influence on the stability and performance of the control system. In this study GA was proposed to tune the PID controller. Results: The results reflect that proposed scheme improves the performance of the process in terms of time domain specifications, robustness to parametric changes and optimum stability. Also, A comparison with the conventional Ziegler-Nichols method proves the superiority of GA based system. Conclusion: This study demonstrates the effectiveness of genetic algorithm in tuning of a PID controller with optimum parameters. It is, moreover, proved to be robust to the variations in plant dynamic characteristics and disturbances assuring a parameter-insensitive operation of the process.

  6. Design of fault tolerant control system for individual blade control helicopters

    Science.gov (United States)

    Tamayo, Sergio

    This dissertation presents the development of a fault tolerant control scheme for helicopters fitted with individually controlled blades. This novel approach attempts to improve fault tolerant capabilities of helicopter control system by increasing control redundancy using additional actuators for individual blade input and software re-mixing to obtain nominal or close to nominal conditions under failure. An advanced interactive simulation environment has been developed including modeling of sensor failure, swashplate actuator failure, individual blade actuator failure, and blade delamination to support the design, testing, and evaluation of the control laws. This simulation environment is based on the blade element theory for the calculation of forces and moments generated by the main rotor. This discretized model allows for individual blade analysis, which in turn allows measuring the consequences of a stuck blade, or loss of the surface area of the blade itself, with respect to the dynamics of the whole helicopter. The control laws are based on non-linear dynamic inversion and artificial neural network augmentation, which is a mix of linear and nonlinear methods that compensates for model inaccuracies due to linearization or failure. A stability analysis based on the Lyapunov function approach has shown that bounded tracking error is guaranteed, and under specific circumstances, global stability is guaranteed as well. An analysis over the degrees of freedom of the mechanical system and its impact over the helicopter handling qualities is also performed to measure the degree of redundancy achieved with the addition of individual blade actuators as compared to a classic swashplate helicopter configuration. Mathematical analysis and numerical simulation, using reconfiguration of the individual blade control under failure have shown that this control architecture can potentially improve the survivability of the aircraft and reduce pilot workload under failure

  7. Towards Robust Predictive Fault–Tolerant Control for a Battery Assembly System

    Directory of Open Access Journals (Sweden)

    Seybold Lothar

    2015-12-01

    Full Text Available The paper deals with the modeling and fault-tolerant control of a real battery assembly system which is under implementation at the RAFI GmbH company (one of the leading electronic manufacturing service providers in Germany. To model and control the battery assembly system, a unified max-plus algebra and model predictive control framework is introduced. Subsequently, the control strategy is enhanced with fault-tolerance features that increase the overall performance of the production system being considered. In particular, it enables tolerating (up to some degree mobile robot, processing and transportation faults. The paper discusses also robustness issues, which are inevitable in real production systems. As a result, a novel robust predictive fault-tolerant strategy is developed that is applied to the battery assembly system. The last part of the paper shows illustrative examples, which clearly exhibit the performance of the proposed approach.

  8. Internal Leakage Fault Detection and Tolerant Control of Single-Rod Hydraulic Actuators

    Directory of Open Access Journals (Sweden)

    Jianyong Yao

    2014-01-01

    Full Text Available The integration of internal leakage fault detection and tolerant control for single-rod hydraulic actuators is present in this paper. Fault detection is a potential technique to provide efficient condition monitoring and/or preventive maintenance, and fault tolerant control is a critical method to improve the safety and reliability of hydraulic servo systems. Based on quadratic Lyapunov functions, a performance-oriented fault detection method is proposed, which has a simple structure and is prone to implement in practice. The main feature is that, when a prescribed performance index is satisfied (even a slight fault has occurred, there is no fault alarmed; otherwise (i.e., a severe fault has occurred, the fault is detected and then a fault tolerant controller is activated. The proposed tolerant controller, which is based on the parameter adaptive methodology, is also prone to realize, and the learning mechanism is simple since only the internal leakage is considered in parameter adaptation and thus the persistent exciting (PE condition is easily satisfied. After the activation of the fault tolerant controller, the control performance is gradually recovered. Simulation results on a hydraulic servo system with both abrupt and incipient internal leakage fault demonstrate the effectiveness of the proposed fault detection and tolerant control method.

  9. Passive Fault-tolerant Control of Discrete-time Piecewise Affine Systems against Actuator Faults

    DEFF Research Database (Denmark)

    Tabatabaeipour, Seyed Mojtaba; Izadi-Zamanabadi, Roozbeh; Bak, Thomas

    2012-01-01

    In this paper, we propose a new method for passive fault-tolerant control of discrete time piecewise affine systems. Actuator faults are considered. A reliable piecewise linear quadratic regulator (LQR) state feedback is designed such that it can tolerate actuator faults. A sufficient condition...... for the exis- tence of a passive fault-tolerant controller is derived and formulated as the feasibility of a set of linear matrix inequalities (LMIs). The upper bound on the performance cost can be minimized using a convex optimization problem with LMI constraints which can be solved efficiently. The approach...

  10. Observer-based fault-tolerant control for a class of nonlinear networked control systems

    Science.gov (United States)

    Mahmoud, M. S.; Memon, A. M.; Shi, Peng

    2014-08-01

    This paper presents a fault-tolerant control (FTC) scheme for nonlinear systems which are connected in a networked control system. The nonlinear system is first transformed into two subsystems such that the unobservable part is affected by a fault and the observable part is unaffected. An observer is then designed which gives state estimates using a Luenberger observer and also estimates unknown parameter of the system; this helps in fault estimation. The FTC is applied in the presence of sampling due to the presence of a network in the loop. The controller gain is obtained using linear-quadratic regulator technique. The methodology is applied on a mechatronic system and the results show satisfactory performance.

  11. Robust fault-tolerant control for wing flutter under actuator failure

    Institute of Scientific and Technical Information of China (English)

    Gao Mingzhou; Cai Guoping

    2016-01-01

    Many control laws, such as optimal controller and classical controller, have seen their applications to suppressing the aeroelastic vibrations of the aeroelastic system. However, those con-trol laws may not work effectively if the aeroelastic system involves actuator faults. In the current study for wing flutter of reentry vehicle, the effect of actuator faults on wing flutter system is rarely considered and few of the fault-tolerant control problems are taken into account. In this paper, we use the radial basis function neural network and the finite-time H∞adaptive fault-tolerant control technique to deal with the flutter problem of wings, which is affected by actuator faults, actuator saturation, parameter uncertainties and external disturbances. The theory of this article includes the modeling of wing flutter and fault-tolerant controller design. The stability of the finite-time adaptive fault-tolerant controller is theoretically proved. Simulation results indicate that the designed fault-tolerant flutter controller can effectively deal with the faults in the flutter system and can promptly suppress the wing flutter as well.

  12. Robust fault-tolerant control for wing flutter under actuator failure

    Directory of Open Access Journals (Sweden)

    Gao Mingzhou

    2016-08-01

    Full Text Available Many control laws, such as optimal controller and classical controller, have seen their applications to suppressing the aeroelastic vibrations of the aeroelastic system. However, those control laws may not work effectively if the aeroelastic system involves actuator faults. In the current study for wing flutter of reentry vehicle, the effect of actuator faults on wing flutter system is rarely considered and few of the fault-tolerant control problems are taken into account. In this paper, we use the radial basis function neural network and the finite-time H∞ adaptive fault-tolerant control technique to deal with the flutter problem of wings, which is affected by actuator faults, actuator saturation, parameter uncertainties and external disturbances. The theory of this article includes the modeling of wing flutter and fault-tolerant controller design. The stability of the finite-time adaptive fault-tolerant controller is theoretically proved. Simulation results indicate that the designed fault-tolerant flutter controller can effectively deal with the faults in the flutter system and can promptly suppress the wing flutter as well.

  13. Fault diagnosis and fault-tolerant control of photovoltaic micro-inverter

    Institute of Scientific and Technical Information of China (English)

    李舟; 彭涛; 张鹏飞; 韩华; 杨建

    2016-01-01

    An observer-based fault diagnosis method and a fault tolerant control for open-switch fault and current sensor fault are proposed for interleaved flyback converters of a micro-inverter system. First, based on the topology of a grid-connected micro-inverter, a mathematical model of the flyback converters is established. Second, a state observer is applied to estimate the currents online and generate corresponding residuals. The fault is diagnosed by comparing the residuals with the thresholds. Finally, a fault-tolerant control that consists of a fault-tolerant topology for the faulty switch and a simple software redundancy control for the faulty current sensor, is proposed to achieve a fault-tolerant operation. The feasibility and effectiveness of the proposed method has been verified by simulation and experimental results.

  14. Fractal gene regulatory networks for robust locomotion control of modular robots

    DEFF Research Database (Denmark)

    Zahadat, Payam; Christensen, David Johan; Schultz, Ulrik Pagh;

    2010-01-01

    Designing controllers for modular robots is difficult due to the distributed and dynamic nature of the robots. In this paper fractal gene regulatory networks are evolved to control modular robots in a distributed way. Experiments with different morphologies of modular robot are performed and the ......Designing controllers for modular robots is difficult due to the distributed and dynamic nature of the robots. In this paper fractal gene regulatory networks are evolved to control modular robots in a distributed way. Experiments with different morphologies of modular robot are performed...

  15. Reliability Assessment Based on Design and Manufacturing Tolerances for Control Burst Mechanism of Small Arms

    Directory of Open Access Journals (Sweden)

    S.K. Basu

    1997-10-01

    Full Text Available Very often specified tolerance is made greater than process tolerance, depending upon (i the manufacturing process capability, and (ii the 'aspiration level' of the designer in effecting a specified tolerance. This applies to multiple components merging into an assembly. In assembly tolerance, errors due to mating are inherent. Common errors arise due to clearance, misalignment in planes and distortion that may cause side stack. Such errors affect the functional performance of the subsystem and consequently become the main cause of failure. Probability distribution of the assembly tolerance and probability distribution of stacked up tolerance of the Components in actual practice leave a common zone of interaction, based on which the in-built reliability changes. From the designer's tolerance, one may have an idea about the 'aspiration level' of assembly tolerance stacking error. Assuming both these parameters, viz., actual stacking error and designer's aspiration level of stacking error to follow the normal probability distribution, it is possible to get the reliability of the product assembly. The paper presents a real life case study for assessing the reliability of sub-assembly at the initial stages of development for control burst mechanism (CBM of rifle.

  16. Usnic acid controls the acidity tolerance of lichens.

    Science.gov (United States)

    Hauck, Markus; Jürgens, Sascha-René

    2008-11-01

    The hypotheses were tested that, firstly, lichens producing the dibenzofuran usnic acid colonize substrates characterized by specific pH ranges, secondly, this preferred pH is in a range where soluble usnic acid and its corresponding anion occur in similar concentrations, and thirdly, usnic acid makes lichens vulnerable to acidity. Lichens with usnic acid prefer an ambient pH range between 3.5 and 5.5 with an optimum between 4.0 and 4.5. This optimum is close to the pK(a1) value of usnic acid of 4.4. Below this optimum pH, dissolved SO(2) reduces the chlorophyll fluorescence yield more in lichens with than without their natural content of usnic acid. This suggests that usnic acid influences the acidity tolerance of lichens. The putative mechanism of the limited acidity tolerance of usnic acid-containing lichens is the acidification of the cytosol by molecules of protonated usnic acid shuttling protons through the plasma membrane at an apoplastic pH

  17. Active fault-tolerant control strategy of large civil aircraft under elevator failures

    Directory of Open Access Journals (Sweden)

    Wang Xingjian

    2015-12-01

    Full Text Available Aircraft longitudinal control is the most important actuation system and its failures would lead to catastrophic accident of aircraft. This paper proposes an active fault-tolerant control (AFTC strategy for civil aircraft with different numbers of faulty elevators. In order to improve the fault-tolerant flight control system performance and effective utilization of the control surface, trimmable horizontal stabilizer (THS is considered to generate the extra pitch moment. A suitable switching mechanism with performance improvement coefficient is proposed to determine when it is worthwhile to utilize THS. Furthermore, AFTC strategy is detailed by using model following technique and the proposed THS switching mechanism. The basic fault-tolerant controller is designed to guarantee longitudinal control system stability and acceptable performance degradation under partial elevators failure. The proposed AFTC is applied to Boeing 747-200 numerical model and simulation results validate the effectiveness of the proposed AFTC approach.

  18. Data-driven design of fault diagnosis and fault-tolerant control systems

    CERN Document Server

    Ding, Steven X

    2014-01-01

    Data-driven Design of Fault Diagnosis and Fault-tolerant Control Systems presents basic statistical process monitoring, fault diagnosis, and control methods, and introduces advanced data-driven schemes for the design of fault diagnosis and fault-tolerant control systems catering to the needs of dynamic industrial processes. With ever increasing demands for reliability, availability and safety in technical processes and assets, process monitoring and fault-tolerance have become important issues surrounding the design of automatic control systems. This text shows the reader how, thanks to the rapid development of information technology, key techniques of data-driven and statistical process monitoring and control can now become widely used in industrial practice to address these issues. To allow for self-contained study and facilitate implementation in real applications, important mathematical and control theoretical knowledge and tools are included in this book. Major schemes are presented in algorithm form and...

  19. On the Controller Synthesis for Markov Decision Process of Conflict Tolerant Specification

    Science.gov (United States)

    Zhang, Junhua; Huang, Zhiqiu; Cao, Zining

    For an embedded control system, different requirements often need be satisfied at same time, and some of them make the system to act conflicted. Conflict tolerant specification is provided to denote this situation. In such a system, there often exist probabilistic and non-deterministic behaviors. We use Markov Decision Process (MDP) to denote these features. We study the controller synthesis for MDP over conflict tolerant specification. We extend PCTL star by adding past operator to denote the conflict tolerant specification succinctly. We use CT-PLTL to denote conflicted actions and PCTL to denote the specification for probability demand. We first synthesize a controller on a base system over CT-PLTL and then use it to prune the corresponding MDP of the system model. We use the resulting sub-MDP as the model to further synthesis a controller over PCTL. The whole controller for MDP is a conjunction of the two controllers obtained.

  20. Active fault-tolerant control strategy of large civil aircraft under elevator failures

    Institute of Scientific and Technical Information of China (English)

    Wang Xingjian; Wang Shaoping; Yang Zhongwei; Zhang Chao

    2015-01-01

    Aircraft longitudinal control is the most important actuation system and its failures would lead to catastrophic accident of aircraft. This paper proposes an active fault-tolerant control (AFTC) strategy for civil aircraft with different numbers of faulty elevators. In order to improve the fault-tolerant flight control system performance and effective utilization of the control surface, trim-mable horizontal stabilizer (THS) is considered to generate the extra pitch moment. A suitable switching mechanism with performance improvement coefficient is proposed to determine when it is worthwhile to utilize THS. Furthermore, AFTC strategy is detailed by using model following technique and the proposed THS switching mechanism. The basic fault-tolerant controller is designed to guarantee longitudinal control system stability and acceptable performance degradation under partial elevators failure. The proposed AFTC is applied to Boeing 747-200 numerical model and simulation results validate the effectiveness of the proposed AFTC approach.

  1. 76 FR 67315 - Supplemental Nutrition Assistance Program: Quality Control Error Tolerance Threshold

    Science.gov (United States)

    2011-11-01

    ... Service 7 CFR Part 275 RIN 0584-AE24 Supplemental Nutrition Assistance Program: Quality Control Error... direct final rule is amending the Quality Control (QC) review error threshold in our regulations from $25... Assistance Program: Quality Control Error Tolerance Threshold Direct Rule, in the subject line of the message...

  2. Robust fault tolerant control of uncertain time-delay linear systems

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Robust fault tolerant control for a class of time-delay linear systems with parameter uncertainties is studied, and a time-delay related state feedback control is proposed. On the basis of Lyapunov method , we prove that the proposed control law has integrity against sensor and/or actuator failures if the correspondent sufficient condition can be satisfied. A heuristic algorithm is also provided to facilitate the realization of the fault tolerant control. Finally, a simulation example is presented to show the effectiveness of the proposed approach.

  3. Industrial Cost-Benefit Assessment for Fault-tolerant Control Systems

    DEFF Research Database (Denmark)

    Thybo, C.; Blanke, M.

    1998-01-01

    Economic aspects are decisive for industrial acceptance of research concepts including the promising ideas in fault tolerant control. Fault tolerance is the ability of a system to detect, isolate and accommodate a fault, such that simple faults in a sub-system do not develop into failures...... at a system level. In a design phase for an industrial system, possibilities span from fail safe design where any single point failure is accommodated by hardware, over fault-tolerant design where selected faults are handled without extra hardware, to fault-ignorant design where no extra precaution is taken....... The objective of this paper is to help, in the early product development state, to find the economical most suitable scheme. A salient result is that with increased customer awareness of total cost of ownership, new products can benefit significantly from applying fault tolerant control principles....

  4. Control of central and peripheral tolerance by Aire.

    Science.gov (United States)

    Metzger, Todd C; Anderson, Mark S

    2011-05-01

    The negative selection of self-reactive thymocytes depends on the expression of tissue-specific antigens by medullary thymic epithelial cells. The autoimmune regulator (Aire) protein plays an important role in turning on these antigens, and the absence of even one Aire-induced tissue-specific antigen in the thymus can lead to autoimmunity in the antigen-expressing target organ. Recently, Aire protein has been detected in peripheral lymphoid organs, suggesting that peripheral Aire plays a complementary role here. In these peripheral sites, Aire was found to regulate the expression of a group of tissue-specific antigens that is distinct from those expressed in the thymus. Furthermore, transgenic antigen expression in extrathymic Aire-expressing cells (eTACs) can mediate deletional tolerance, but the immunological relevance of Aire-dependent, endogenous tissue-specific antigens remains to be determined. © 2011 John Wiley & Sons A/S.

  5. Integral Sliding Mode Fault-Tolerant Control for Uncertain Linear Systems Over Networks With Signals Quantization.

    Science.gov (United States)

    Hao, Li-Ying; Park, Ju H; Ye, Dan

    2016-06-13

    In this paper, a new robust fault-tolerant compensation control method for uncertain linear systems over networks is proposed, where only quantized signals are assumed to be available. This approach is based on the integral sliding mode (ISM) method where two kinds of integral sliding surfaces are constructed. One is the continuous-state-dependent surface with the aim of sliding mode stability analysis and the other is the quantization-state-dependent surface, which is used for ISM controller design. A scheme that combines the adaptive ISM controller and quantization parameter adjustment strategy is then proposed. Through utilizing H∞ control analytical technique, once the system is in the sliding mode, the nature of performing disturbance attenuation and fault tolerance from the initial time can be found without requiring any fault information. Finally, the effectiveness of our proposed ISM control fault-tolerant schemes against quantization errors is demonstrated in the simulation.

  6. Pharyngeal mesoderm regulatory network controls cardiac and head muscle morphogenesis.

    Science.gov (United States)

    Harel, Itamar; Maezawa, Yoshiro; Avraham, Roi; Rinon, Ariel; Ma, Hsiao-Yen; Cross, Joe W; Leviatan, Noam; Hegesh, Julius; Roy, Achira; Jacob-Hirsch, Jasmine; Rechavi, Gideon; Carvajal, Jaime; Tole, Shubha; Kioussi, Chrissa; Quaggin, Susan; Tzahor, Eldad

    2012-11-13

    The search for developmental mechanisms driving vertebrate organogenesis has paved the way toward a deeper understanding of birth defects. During embryogenesis, parts of the heart and craniofacial muscles arise from pharyngeal mesoderm (PM) progenitors. Here, we reveal a hierarchical regulatory network of a set of transcription factors expressed in the PM that initiates heart and craniofacial organogenesis. Genetic perturbation of this network in mice resulted in heart and craniofacial muscle defects, revealing robust cross-regulation between its members. We identified Lhx2 as a previously undescribed player during cardiac and pharyngeal muscle development. Lhx2 and Tcf21 genetically interact with Tbx1, the major determinant in the etiology of DiGeorge/velo-cardio-facial/22q11.2 deletion syndrome. Furthermore, knockout of these genes in the mouse recapitulates specific cardiac features of this syndrome. We suggest that PM-derived cardiogenesis and myogenesis are network properties rather than properties specific to individual PM members. These findings shed new light on the developmental underpinnings of congenital defects.

  7. Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts.

    Directory of Open Access Journals (Sweden)

    Yuta Abe

    Full Text Available BACKGROUND: Donor-specific blood transfusion (DST prior to solid organ transplantation has been shown to induce long-term allograft survival in the absence of immunosuppressive therapy. Although the mechanisms underlying DST-induced allograft tolerance are not well defined, there is evidence to suggest DST induces one or more populations of antigen-specific regulatory cells that suppress allograft rejection. However, neither the identity nor the regulatory properties of these tolerogenic lymphocytes have been reported. Therefore, the objective of this study was to define the kinetics, phenotype and suppressive function of the regulatory cells induced by DST alone or in combination with liver allograft transplantation (LTx. METHODOLOGY/PRINCIPAL FINDINGS: Tolerance to Dark Agouti (DA; RT1(a rat liver allografts was induced by injection (iv of 1 ml of heparinized DA blood to naïve Lewis (LEW; RT1(l rats once per week for 4 weeks prior to LTx. We found that preoperative DST alone generates CD4(+ T-cells that when transferred into naïve LEW recipients are capable of suppressing DA liver allograft rejection and promoting long-term survival of the graft and recipient. However, these DST-generated T-cells did not express the regulatory T-cell (Treg transcription factor Foxp3 nor did they suppress alloantigen (DA-induced activation of LEW T-cells in vitro suggesting that these lymphocytes are not fully functional regulatory Tregs. We did observe that DST+LTx (but not DST alone induced the time-dependent formation of CD4(+Foxp3(+ Tregs that potently suppressed alloantigen-induced activation of naïve LEW T-cells in vitro and liver allograft rejection in vivo. Finally, we present data demonstrating that virtually all of the Foxp3-expressing Tregs reside within the CD4(+CD45RC(- population whereas in which approximately 50% of these Tregs express CD25. CONCLUSIONS/SIGNIFICANCE: We conclude that preoperative DST, in the absence of liver allograft

  8. Variability control: key to regulatory compliance and sustainability goals.

    Science.gov (United States)

    Englande, A J; Eckenfelder, W W; Jin, G

    2006-01-01

    The focus of this paper is on variability concerns in wastewater treatment and approaches to control unacceptable fluctuations in effluent quality. Areas considered include: factors contributing to variability in both waste loads and process technology performance; variability assessment; control of variability employing the process best management practice (BMP); design/operation of biological waste treatment technologies for variability reduction; and modelling to enhance process control.

  9. Modeling the Fault Tolerant Capability of a Flight Control System: An Exercise in SCR Specification

    Science.gov (United States)

    Alexander, Chris; Cortellessa, Vittorio; DelGobbo, Diego; Mili, Ali; Napolitano, Marcello

    2000-01-01

    In life-critical and mission-critical applications, it is important to make provisions for a wide range of contingencies, by providing means for fault tolerance. In this paper, we discuss the specification of a flight control system that is fault tolerant with respect to sensor faults. Redundancy is provided by analytical relations that hold between sensor readings; depending on the conditions, this redundancy can be used to detect, identify and accommodate sensor faults.

  10. Induction of tolerance via T regulatory cells. A clinical reality or just a nice experiment in mice?

    NARCIS (Netherlands)

    Battaglia, M.

    2014-01-01

    T regulatory (Treg) cells are a subset of lymphocytes specifically dedicated to maintain the correct balance between efficient immune responses towards harmful events (such as a viral infections) and tolerogenic responses towards self and non-harmless antigens (such as food antigens). This is a deli

  11. Integrated design of fault reconstruction and fault-tolerant control against actuator faults using learning observers

    Science.gov (United States)

    Jia, Qingxian; Chen, Wen; Zhang, Yingchun; Li, Huayi

    2016-12-01

    This paper addresses the problem of integrated fault reconstruction and fault-tolerant control in linear systems subject to actuator faults via learning observers (LOs). A reconfigurable fault-tolerant controller is designed based on the constructed LO to compensate for the influence of actuator faults by stabilising the closed-loop system. An integrated design of the proposed LO and the fault-tolerant controller is explored such that their performance can be simultaneously considered and their coupling problem can be effectively solved. In addition, such an integrated design is formulated in terms of linear matrix inequalities (LMIs) that can be conveniently solved in a unified framework using LMI optimisation technique. At last, simulation studies on a micro-satellite attitude control system are provided to verify the effectiveness of the proposed approach.

  12. A modal H∞-norm-based performance requirement for damage-tolerant active controller design

    Science.gov (United States)

    Genari, Helói F. G.; Mechbal, Nazih; Coffignal, Gérard; Nóbrega, Eurípedes G. O.

    2017-04-01

    Damage-tolerant active control (DTAC) is a recent research area that encompasses control design methodologies resulting from the application of fault-tolerant control methods to vibration control of structures subject to damage. The possibility of damage occurrence is not usually considered in the active vibration control design requirements. Damage changes the structure dynamics, which may produce unexpected modal behavior of the closed-loop system, usually not anticipated by the controller design approaches. A modal H∞ norm and a respective robust controller design framework were recently introduced, and this method is here extended to face a new DTAC strategy implementation. Considering that damage affects each vibration mode differently, this paper adopts the modal H∞ norm to include damage as a design requirement. The basic idea is to create an appropriate energy distribution over the frequency range of interest and respective vibration modes, guaranteeing robustness, damage tolerance, and adequate overall performance, taking into account that it is common to have previous knowledge of the structure regions where damage may occur during its operational life. For this purpose, a structural health monitoring technique is applied to evaluate modal modifications caused by damage. This information is used to create modal weighing matrices, conducting to the modal H∞ controller design. Finite element models are adopted for a case study structure, including different damage severities, in order to validate the proposed control strategy. Results show the effectiveness of the proposed methodology with respect to damage tolerance.

  13. Organization, Integration and Assembly of Genetic and Epigenetic Regulatory Machinery in Nuclear Microenvironments: Implications for Biological Control in Cancer

    OpenAIRE

    Stein, Gary S.; Zaidi, Sayyed K.; Stein, Janet L.; Lian, Jane B; van Wijnen, Andre; Montecino, Martin; Young, Daniel W.; Javed, Amjad; Pratap, Jitesh; Choi, Je-Yong; Ali, Syed A; Pande, Sandhya; Hassan, Mohammad Q.

    2009-01-01

    There is growing awareness that the fidelity of gene expression necessitates coordination of transcription factor metabolism and organization of genes and regulatory proteins within the three dimensional context of nuclear architecture. The regulatory machinery that governs genetic and epigenetic control of gene expression is compartmentalized in nuclear microenvironments. Temporal and spatial parameters of regulatory complex organization and assembly are functionally linked to biological con...

  14. Robust Adaptive Fault-Tolerant Tracking Control of Three-Phase Induction Motor

    Directory of Open Access Journals (Sweden)

    Hossein Tohidi

    2014-01-01

    Full Text Available This paper deals with the problem of induction motor tracking control against actuator faults and external disturbances using the linear matrix inequalities (LMIs method and the adaptive method. A direct adaptive fault-tolerant tracking controller design method is developed based on Lyapunov stability theory and a constructive algorithm based on linear matrix inequalities for online tuning of adaptive and state feedback gains to stabilize the closed-loop system in order to reduce the fault effect with disturbance attenuation. Simulation results reveal the merits of proposed robust adaptive fault-tolerant tracking control scheme on an induction motor subjected to actuator faults.

  15. Cold tolerance of bed bugs and practical recommendations for control.

    Science.gov (United States)

    Olson, Joelle F; Eaton, Marc; Kells, Stephen A; Morin, Victor; Wang, Changlu

    2013-12-01

    Bed bugs were exposed to freezing temperatures for various exposure times to determine cold tolerance and mortality estimates for multiple life stages. The mean supercooling point for all bed bug life stages ranged from -21.3 degrees C to -30.3 degrees C, with the egg stage reporting the lowest value. A probit analysis provided a lower lethal temperature (LLT99) of -31.2 degrees C when estimates from all life stages were combined, demonstrating that all stages of bed bugs are not capable of surviving temperatures below body freezing and are therefore freeze intolerant. At conditions above the LLT99, bed bug mortality depended on temperature and exposure time at temperatures above LLT99. Based on our model estimates, survival was estimated for temperatures above -12 degrees C even after 1 wk of continuous exposure. However, exposure to temperatures below -13 degrees C will result in 100% mortality in d to ensure mortality of all life stages. Unfortunately, sublethal exposure to lower temperatures did not prevent subsequent feeding behavior in surviving stages. Practical recommendations for management of potentially infested items are discussed.

  16. Can Airway Tolerance be Promoted Immunopharmacologically with Aspirin in Aspirin-insensitive Allergic Bonchial Asthmatics by T Regulatory Cells (Tregs-directed Immunoregulatory Therapy?

    Directory of Open Access Journals (Sweden)

    Muzammal Hussain

    2012-07-01

    Full Text Available The pathobiology of allergic bronchial asthma is mediated by over-expressed T helper type 2 (Th2-biased immune responses to harmless environmental antigens, leading to airway inflammation and hyper-responsiveness. These Th2 responses are normally suppressed by functional T regulatory cells (Tregs, which maintain the airway tolerance. However, the Tregs activity is conceived to be compromised in allergic asthmatics. The curative therapy to counteract this immune dysregulation is not available so far, and to devise such a remedy is the current research impetus in allergic asthma therapeutics. One of the novel insights is to consider a Tregs-directed immunoregulatory therapy that could harness endogenous Tregs to redress the Th2/Tregs imbalance, thus enhancing the airway tolerance. Aspirin or acetylsalicylic acid (ASA is a prototype non-steroidal anti-inflammatory drug that possesses intriguing immunopharmacological attributes. For example, it can enhance the number or the frequency of functional Tregs, especially natural CD4+ CD25+ FoxP3+ Tregs, either directly or by inducing tolerogenic activity in dendritic cells (DCs. It is also considered to be beneficial for the induction of immunological tolerance in autoimmunity and graft rejection. This raises the question whether ASA, if exploited optimally, may be used to induce and harness endogenous Tregs activity for redressing Th2/Tregs imbalance in allergic asthma. In this paper, we hypothesise that ASA may help to counteract the underlying immune dysregulation in allergic asthma by promoting airway tolerance. Nevertheless, the future research in this regard will selectively need to be targeted to allergic asthma models, which are ASA insensitive, as ASA has some adverse background and is contraindicated in asthmatics who are sensitive to it.

  17. A Cyclin Dependent Kinase Regulatory Subunit (CKS) Gene of Pigeonpea Imparts Abiotic Stress Tolerance and Regulates Plant Growth and Development in Arabidopsis.

    Science.gov (United States)

    Tamirisa, Srinath; Vudem, Dashavantha R; Khareedu, Venkateswara R

    2017-01-01

    Frequent climatic changes in conjunction with other extreme environmental factors are known to affect growth, development and productivity of diverse crop plants. Pigeonpea, a major grain legume of the semiarid tropics, endowed with an excellent deep-root system, is known as one of the important drought tolerant crop plants. Cyclin dependent kinases (CDKs) are core cell cycle regulators and play important role in different aspects of plant growth and development. The cyclin-dependent kinase regulatory subunit gene (CKS) was isolated from the cDNA library of pigeonpea plants subjected to drought stress. Pigeonpea CKS (CcCKS) gene expression was detected in both the root and leaf tissues of pigeonpea and was upregulated by polyethylene glycol (PEG), mannitol, NaCl and abscisic acid (ABA) treatments. The overexpression of CcCKS gene in Arabidopsis significantly enhanced tolerance of transgenics to drought and salt stresses as evidenced by different physiological parameters. Under stress conditions, transgenics showed higher biomass, decreased rate of water loss, decreased MDA levels, higher free proline contents, and glutathione levels. Moreover, under stress conditions transgenics exhibited lower stomatal conductance, lower transpiration, and higher photosynthetic rates. However, under normal conditions, CcCKS-transgenics displayed decreased plant growth rate, increased cell size and decreased stomatal number compared to those of wild-type plants. Real-time polymerase chain reaction revealed that CcCKS could regulate the expression of both ABA-dependent and ABA-independent genes associated with abiotic stress tolerance as well as plant growth and development. As such, the CcCKS seems promising and might serve as a potential candidate gene for enhancing the abiotic stress tolerance of crop plants.

  18. Anti-CD154 mAb and rapamycin induce T regulatory cell mediated tolerance in rat-to-mouse islet transplantation.

    Directory of Open Access Journals (Sweden)

    Yannick D Muller

    Full Text Available BACKGROUND: Anti-CD154 (MR1 monoclonal antibody (mAb and rapamycin (RAPA treatment both improve survival of rat-to-mouse islet xenograft. The present study investigated the effect of combined RAPA/MR1 treatment on rat-to-mouse islet xenograft survival and analyzed the role of CD4(+CD25(+Foxp3(+ T regulatory cells (Treg in the induction and maintenance of the ensuing tolerance. METHODOLOGY/PRINCIPAL FINDINGS: C57BL/6 mice were treated with MR1/RAPA and received additional monoclonal anti-IL2 mAb or anti CD25 mAb either early (0-28 d or late (100-128 d post-transplantation. Treg were characterised in the blood, spleen, draining lymph nodes and within the graft of tolerant and rejecting mice by flow cytometry and immunohistochemistry. Fourteen days of RAPA/MR1 combination therapy allowed indefinite islet graft survival in >80% of the mice. Additional administration of anti-IL-2 mAb or depleting anti-CD25 mAb at the time of transplantation resulted in rejection (100% and 89% respectively, whereas administration at 100 days post transplantation lead to lower rejection rates (25% and 40% respectively. Tolerant mice showed an increase of Treg within the graft and in draining lymph nodes early post transplantation, whereas 100 days post transplantation no significant increase of Treg was observed. Rejecting mice showed a transient increase of Treg in the xenograft and secondary lymphoid organs, which disappeared within 7 days after rejection. CONCLUSIONS/SIGNIFICANCES: These results suggest a critical role for Treg in the induction phase of tolerance early after islet xenotransplantation. These encouraging data support the need of developing further Treg therapy for overcoming the species barrier in xenotransplantation.

  19. Robust and Fault-Tolerant Linear Parameter-Varying Control of Wind Turbines

    DEFF Research Database (Denmark)

    Sloth, Christoffer; Esbensen, Thomas; Stoustrup, Jakob

    2011-01-01

    , designed using a proposed method that allows the inclusion of both faults and uncertainties in the LPV controller design. We specifically consider a 4.8 MW, variable-speed, variable-pitch wind turbine model with a fault in the pitch system. We propose the design of a nominal controller (NC), handling...... the parameter variations along the nominal operating trajectory caused by nonlinear aerodynamics. To accommodate the fault in the pitch system, an active fault-tolerant controller (AFTC) and a passive fault-tolerant controller (PFTC) are designed. In addition to the nominal LPV controller, we also propose...... a robust controller (RC). This controller is able to take into account model uncertainties in the aerodynamic model. The controllers are based on output feedback and are scheduled on an estimated wind speed to manage the parameter-varying nature of the model. Furthermore, the AFTC relies on information...

  20. Fault tolerant control of wind turbines using unknown input observers

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Stoustrup, Jakob

    2012-01-01

    This paper presents a scheme for accommodating faults in the rotor and generator speed sensors in a wind turbine. These measured values are important both for the wind turbine controller as well as the supervisory control of the wind turbine. The scheme is based on unknown input observers, which...

  1. Subnuclear organization and trafficking of regulatory proteins: implications for biological control and cancer.

    Science.gov (United States)

    Stein, G S; van Wijnen, A J; Stein, J L; Lian, J B; Montecino, M; Zaidi, K; Javed, A

    2000-01-01

    The regulated and regulatory components that interrelate nuclear structure and function must be experimentally established. A formidable challenge is to define further the control of transcription factor targeting to acceptor sites associated with the nuclear matrix. It will be important to determine whether acceptor proteins are associated with a pre-existing core-filament structural lattice or whether a compositely organized scaffold of regulatory factors is dynamically assembled. An inclusive model for all steps in the targeting of proteins to subnuclear sites cannot yet be proposed. However, this model must account for the apparent diversity of intranuclear targeting signals. It is also important to assess the extent to which regulatory discrimination is mediated by subnuclear domain-specific trafficking signals. Furthermore, the checkpoints that monitor subnuclear distribution of regulatory factors and the sorting steps that ensure both structural and functional fidelity of nuclear domains in which replication and expression of genes occur must be biochemically and mechanistically defined. There is emerging recognition that placement of regulatory components of gene expression must be temporally and spatially coordinated to facilitate biological control. The consequences of breaches in nuclear structure-function relationships are observed in an expanding series of diseases that include cancer [Weis et al., 1994; Rogaia et al., 1997; Yano et al., 1997; Rowley, 1998; Zeng et al., 1998; McNeil et al., 1999; Tao and Levine, 1999a] and neurological disorders [Skinner et al., 1997]. As the repertoire of architecture-associated regulatory factors and cofactors expands, workers in the field are becoming increasingly confident that nuclear organization contributes significantly to control of transcription. To gain increased appreciation for the complexities of subnuclear organization and gene regulation, we must continue to characterize mechanisms that direct

  2. Novel regulatory cascades controlling expression of nitrogen-fixation genes in Geobacter sulfurreducens.

    Science.gov (United States)

    Ueki, Toshiyuki; Lovley, Derek R

    2010-11-01

    Geobacter species often play an important role in bioremediation of environments contaminated with metals or organics and show promise for harvesting electricity from waste organic matter in microbial fuel cells. The ability of Geobacter species to fix atmospheric nitrogen is an important metabolic feature for these applications. We identified novel regulatory cascades controlling nitrogen-fixation gene expression in Geobacter sulfurreducens. Unlike the regulatory mechanisms known in other nitrogen-fixing microorganisms, nitrogen-fixation gene regulation in G. sulfurreducens is controlled by two two-component His-Asp phosphorelay systems. One of these systems appears to be the master regulatory system that activates transcription of the majority of nitrogen-fixation genes and represses a gene encoding glutamate dehydrogenase during nitrogen fixation. The other system whose expression is directly activated by the master regulatory system appears to control by antitermination the expression of a subset of the nitrogen-fixation genes whose transcription is activated by the master regulatory system and whose promoter contains transcription termination signals. This study provides a new paradigm for nitrogen-fixation gene regulation.

  3. Fault Tolerant Control Design for the Longitudinal Aircraft Dynamics using Quantitative Feedback Theory

    OpenAIRE

    Ossmann, Daniel

    2015-01-01

    Flight control laws of modern aircraft are scheduled with respect to flight point parameters. The loss of the air data measurement system implies inevitably the loss of relevant scheduling information. A strategy to design a fault tolerant longitudinal flight control system is proposed which can accommodate the total loss of the angle of attack and the calibrated airspeed measurements. In this scenario the described robust longitudinal control law is employed ensuring a control performance ...

  4. Combining Artificial Intelligence and Robust Techniques with MRAC in Fault Tolerant Control

    OpenAIRE

    Vargas Martínez, Adriana

    2011-01-01

    The investigation of this thesis presents different approaches for Fault Tolerant Control based on Model Reference Adaptive Control, Artificial Neural Networks, PID controller optimized by a Genetic Algorithm, Nonlinear, Robust and Linear Parameter Varying (LPV) control for Linear Time Invariant (LTI), LPV and nonlinear systems. All of the above techniques are integrated in different controller�s structures to prove their ability to accommodate a fault. Modern systems and their challenging op...

  5. Regulatory genes controlling mitosis in the fission yeast Schizosaccharomyces pombe.

    Science.gov (United States)

    Nurse, P; Thuriaux, P

    1980-11-01

    Fifty-two wee mutants that undergo mitosis and cell division at a reduced size compared with wild type have been genetically analyzed. The mutants define two genes, wee1 and cdc2, which control the timing of mitosis. Fifty-one of the mutants map at the wee1 locus, which is unlinked to any known cdc gene. One of the wee1 alleles has been shown to be nonsense suppressible. The 52nd were mutant maps within cdc2. Previously, only temperature-sensitive mutants that become blocked at mitosis have been found at the cdc2 locus. The simplest interpretation of these observations is that wee1+ codes for a negative element or inhibitor, and cdc2+ codes for a positive element or activator in the mitotic control. The gene dosage of wee1+ plays some role in determining the timing of mitosis, but the gene dosage of cdc2+ has little effect. However, some aspect of the cdc2 gene product activity is important for determining when mitosis takes place. The possible roles of wee1 and cdc2 in the mitotic control are discussed, with particular reference to the part they may play in the monitoring of cell growth rate, both of which influence the timing of mitosis.

  6. Robust fault-tolerant H∞ control of active suspension systems with finite-frequency constraint

    Science.gov (United States)

    Wang, Rongrong; Jing, Hui; Karimi, Hamid Reza; Chen, Nan

    2015-10-01

    In this paper, the robust fault-tolerant (FT) H∞ control problem of active suspension systems with finite-frequency constraint is investigated. A full-car model is employed in the controller design such that the heave, pitch and roll motions can be simultaneously controlled. Both the actuator faults and external disturbances are considered in the controller synthesis. As the human body is more sensitive to the vertical vibration in 4-8 Hz, robust H∞ control with this finite-frequency constraint is designed. Other performances such as suspension deflection and actuator saturation are also considered. As some of the states such as the sprung mass pitch and roll angles are hard to measure, a robust H∞ dynamic output-feedback controller with fault tolerant ability is proposed. Simulation results show the performance of the proposed controller.

  7. Regulatory Genes Controlling Mitosis in the Fission Yeast SCHIZOSACCHAROMYCES POMBE

    OpenAIRE

    Nurse, Paul; Thuriaux, Pierre

    1980-01-01

    Fifty-two wee mutants that undergo mitosis and cell division at a reduced size compared with wild type have been genetically analyzed. The mutants define two genes, wee1 and cdc2, which control the timing of mitosis. Fifty-one of the mutants map at the wee1 locus, which is unlinked to any known cdc gene. One of the wee1 alleles has been shown to be nonsense suppressible. The 52nd wee mutant maps within cdc2. Previously, only temperature-sensitive mutants that become blocked at mitosis have be...

  8. The national and international regulatory environment in tobacco control.

    Science.gov (United States)

    Warner, Kenneth E

    2015-07-09

    Despite their lethality, cigarettes are subject to little regulation that directly restricts their contents or their legality. This may change in the near future with the Framework Convention on Tobacco Control (FCTC), the world's first global health treaty, now in force, as well as developments in a few individual countries. Cigarettes are subject to a substantial number of country-specific regulations regarding their conditions of sale: their price (mostly through taxation), the places where they can be consumed (clean indoor air laws), who can smoke them (prohibitions on their use by or sales to minors), how they can be advertised or promoted (if at all), and how they must be packaged (minimum pack sizes, warning labels, plain packaging). Such policies constitute the core of successful tobacco control. The FCTC has been ratified by 180 countries representing 90% of the world's population. The FCTC requires compliance with numerous provisions relating to the kinds of regulations noted above. The treaty also mandates explicit attention to direct product regulation. Several countries have such authority, at least in limited forms. In the US, for example, the Food and Drug Administration (FDA) now has the legal authority to regulate tobacco products, including their contents. The possibility exists that, in the foreseeable future, a country will mandate product standards that will substantially reduce the appeal of cigarettes and other combusted tobacco products, which are by far the leading sources of the death and disease associated with tobacco.

  9. Fault Tolerant Emergency Control to Preserve Power System Stability

    DEFF Research Database (Denmark)

    Pedersen, Andreas Søndergaard; Richter, Jan H.; Tabatabaeipour, Mojtaba;

    2016-01-01

    This paper introduces a method for fault-masking and system reconfiguration in power transmission systems. The paper demonstrates how faults are handled by reconfiguring remaining controls through utilisation of wide-area measurement in real time. It is shown how reconfiguration can be obtained...... using a virtual actuator concept, which covers Lure-type systems. The paper shows the steps needed to calculate a virtual actuator, which relies on the solution of a linear matrix inequality. The solution is shown to work with existing controls by adding a compensation signal. Simulation results...... of a benchmark system show ability of the reconfiguration to maintain stability...

  10. Identification of regulatory network hubs that control lipid metabolism in Chlamydomonas reinhardtii.

    Science.gov (United States)

    Gargouri, Mahmoud; Park, Jeong-Jin; Holguin, F Omar; Kim, Min-Jeong; Wang, Hongxia; Deshpande, Rahul R; Shachar-Hill, Yair; Hicks, Leslie M; Gang, David R

    2015-08-01

    Microalgae-based biofuels are promising sources of alternative energy, but improvements throughout the production process are required to establish them as economically feasible. One of the most influential improvements would be a significant increase in lipid yields, which could be achieved by altering the regulation of lipid biosynthesis and accumulation. Chlamydomonas reinhardtii accumulates oil (triacylglycerols, TAG) in response to nitrogen (N) deprivation. Although a few important regulatory genes have been identified that are involved in controlling this process, a global understanding of the larger regulatory network has not been developed. In order to uncover this network in this species, a combined omics (transcriptomic, proteomic and metabolomic) analysis was applied to cells grown in a time course experiment after a shift from N-replete to N-depleted conditions. Changes in transcript and protein levels of 414 predicted transcription factors (TFs) and transcriptional regulators (TRs) were monitored relative to other genes. The TF and TR genes were thus classified by two separate measures: up-regulated versus down-regulated and early response versus late response relative to two phases of polar lipid synthesis (before and after TAG biosynthesis initiation). Lipidomic and primary metabolite profiling generated compound accumulation levels that were integrated with the transcript dataset and TF profiling to produce a transcriptional regulatory network. Evaluation of this proposed regulatory network led to the identification of several regulatory hubs that control many aspects of cellular metabolism, from N assimilation and metabolism, to central metabolism, photosynthesis and lipid metabolism.

  11. Fault tolerant wind turbine production operation and shutdown (Sustainable Control)

    NARCIS (Netherlands)

    Van Engelen, T.; Schuurmans, J.; Kanev, S.; Dong, J.; Verhaegen, M.H.G.; Hayashi, Y.

    2011-01-01

    Extreme environmental conditions as well as system failure are real-life phenomena. Especially offshore, extreme environmental conditions and system faults are to be dealt with in an effective way. The project Sustainable Control, a new approach to operate wind turbines (Agentschap NL, grant EOSLT02

  12. Fault tolerant computer control for a Maglev transportation system

    Science.gov (United States)

    Lala, Jaynarayan H.; Nagle, Gail A.; Anagnostopoulos, George

    1994-01-01

    Magnetically levitated (Maglev) vehicles operating on dedicated guideways at speeds of 500 km/hr are an emerging transportation alternative to short-haul air and high-speed rail. They have the potential to offer a service significantly more dependable than air and with less operating cost than both air and high-speed rail. Maglev transportation derives these benefits by using magnetic forces to suspend a vehicle 8 to 200 mm above the guideway. Magnetic forces are also used for propulsion and guidance. The combination of high speed, short headways, stringent ride quality requirements, and a distributed offboard propulsion system necessitates high levels of automation for the Maglev control and operation. Very high levels of safety and availability will be required for the Maglev control system. This paper describes the mission scenario, functional requirements, and dependability and performance requirements of the Maglev command, control, and communications system. A distributed hierarchical architecture consisting of vehicle on-board computers, wayside zone computers, a central computer facility, and communication links between these entities was synthesized to meet the functional and dependability requirements on the maglev. Two variations of the basic architecture are described: the Smart Vehicle Architecture (SVA) and the Zone Control Architecture (ZCA). Preliminary dependability modeling results are also presented.

  13. Feedback Control of Two-Component Regulatory Systems.

    Science.gov (United States)

    Groisman, Eduardo A

    2016-09-08

    Two-component systems are a dominant form of bacterial signal transduction. The prototypical two-component system consists of a sensor that responds to a specific input(s) by modifying the output of a cognate regulator. Because the output of a two-component system is the amount of phosphorylated regulator, feedback mechanisms may alter the amount of regulator, and/or modify the ability of a sensor or other proteins to alter the phosphorylation state of the regulator. Two-component systems may display intrinsic feedback whereby the amount of phosphorylated regulator changes under constant inducing conditions and without the participation of additional proteins. Feedback control allows a two-component system to achieve particular steady-state levels, to reach a given steady state with distinct dynamics, to express coregulated genes in a given order, and to activate a regulator to different extents, depending on the signal acting on the sensor.

  14. Development of fault tolerant adaptive control laws for aerospace systems

    Science.gov (United States)

    Perez Rocha, Andres E.

    The main topic of this dissertation is the design, development and implementation of intelligent adaptive control techniques designed to maintain healthy performance of aerospace systems subjected to malfunctions, external parameter changes and/or unmodeled dynamics. The dissertation is focused on the development of novel adaptive control configurations that rely on non-linear functions that appear in the immune system of living organisms as main source of adaptation. One of the main goals of this dissertation is to demonstrate that these novel adaptive control architectures are able to improve overall performance and protect the system while reducing control effort and maintaining adequate operation outside bounds of nominal design. This research effort explores several phases, ranging from theoretical stability analysis, simulation and hardware implementation on different types of aerospace systems including spacecraft, aircraft and quadrotor vehicles. The results presented in this dissertation are focused on two main adaptivity approaches, the first one is intended for aerospace systems that do not attain large angles and use exact feedback linearization of Euler angle kinematics. A proof of stability is presented by means of the circle Criterion and Lyapunov's direct method. The second approach is intended for aerospace systems that can attain large attitude angles (e.g. space systems in gravity-less environments), the adaptation is incorporated on a baseline architecture that uses partial feedback linearization of quaternions kinematics. In this case, the closed loop stability was analyzed using Lyapunov's direct method and Barbalat's Lemma. It is expected that some results presented in this dissertation can contribute towards the validation and certification of direct adaptive controllers.

  15. Physiological hemostasis based intelligent integrated cooperative controller for precise fault-tolerant control of redundant parallel manipulator

    Science.gov (United States)

    Hao, Kuangrong; Guo, Chongbin; Ding, Yongsheng

    2014-10-01

    This paper focuses on precise fault-tolerant control for actual redundant parallel manipulator. Based on kinematic redundancy, some unnoticed influences such as mechanical clearance have been considered to design a more precise and intelligent fault-tolerant plan for actual plants. According to regulation principles in human hemostasis system, a bio-inspired intelligent integrated cooperative controller (BIICC) is developed including system structure, algorithm and step in parameter tuning. The proposed BIICC optimises partial error signal and improves control performance in each sub-channel. Moreover, the new controller transfers and disposes cooperative control signals among different sub-channels to achieve an intelligent integrated fault-tolerant system. The proposed BIICC is applied to an actual 2-DOF (degrees of freedom) redundant parallel manipulator where the feasibility of the new controller is demonstrated. The BIICC is beneficial to control precision and fault-tolerant capability of redundant plant. The improvements are more obvious in cases where extra actuators of redundant manipulator are broken.

  16. Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes

    DEFF Research Database (Denmark)

    Ventre, I.; Goodman, A.L.; Vallet-Gely, I.

    2006-01-01

    by controlling the transcription of a small regulatory RNA, RsmZ. This work identifies a previously undescribed signal transduction network in which the activities of signal-receiving sensor kinases LadS, RetS, and GacS regulate expression of virulence genes associated with acute or chronic infection...

  17. Active fault tolerant control for vertical tail damaged aircraft with dissimilar redundant actuation system

    Institute of Scientific and Technical Information of China (English)

    Wang Jun; Wang Shaoping; Wang Xingjian; Shi Cun; Mileta M. Tomovic

    2016-01-01

    This paper proposes an active fault-tolerant control strategy for an aircraft with dissim-ilar redundant actuation system (DRAS) that has suffered from vertical tail damage. A damage degree coefficient based on the effective vertical tail area is introduced to parameterize the damaged flight dynamic model. The nonlinear relationship between the damage degree coefficient and the corresponding stability derivatives is considered. Furthermore, the performance degradation of new input channel with electro-hydrostatic actuator (EHA) is also taken into account in the dam-aged flight dynamic model. Based on the accurate damaged flight dynamic model, a composite method of linear quadratic regulator (LQR) integrating model reference adaptive control (MRAC) is proposed to reconfigure the fault-tolerant control law. The numerical simulation results validate the effectiveness of the proposed fault-tolerant control strategy with accurate flight dynamic model. The results also indicate that aircraft with DRAS has better fault-tolerant control ability than the traditional ones when the vertical tail suffers from serious damage.

  18. Perception and regulatory principles of microbial growth control.

    Science.gov (United States)

    Khonsari, Armin S; Kollmann, Markus

    2015-01-01

    Fast growth represents an effective strategy for microbial organisms to survive in competitive environments. To accomplish this task, cells must adapt their metabolism to changing nutrient conditions in a way that maximizes their growth rate. However, the regulation of the growth related metabolic pathways can be fundamentally different among microbes. We therefore asked whether growth control by perception of the cell's intracellular metabolic state can give rise to higher growth than by direct perception of extracellular nutrient availability. To answer this question, we created a simplified dynamical computer model of a cellular metabolic network whose regulation was inferred by an optimization approach. We used this model for a competing species experiment, where a species with extracellular nutrient perception competes against one with intracellular nutrient perception by evaluating their respective average growth rate. We found that the intracellular perception is advantageous under situations where the up and down regulation of pathways cannot follow the fast changing nutrient availability in the environment. In this case, optimal regulation ignores any other nutrients except the most preferential ones, in agreement with the phenomenon of catabolite repression in prokaryotes. The corresponding metabolic pathways remain activated, despite environmental fluctuations. Therefore, the cell can take up preferential nutrients as soon as they are available without any prior regulation. As a result species that rely on intracellular perception gain a relevant fitness advantage in fluctuating nutrient environments, which enables survival by outgrowing competitors.

  19. The Regulatory Networks That Control Clostridium difficile Toxin Synthesis

    Science.gov (United States)

    Martin-Verstraete, Isabelle; Peltier, Johann; Dupuy, Bruno

    2016-01-01

    The pathogenic clostridia cause many human and animal diseases, which typically arise as a consequence of the production of potent exotoxins. Among the enterotoxic clostridia, Clostridium difficile is the main causative agent of nosocomial intestinal infections in adults with a compromised gut microbiota caused by antibiotic treatment. The symptoms of C. difficile infection are essentially caused by the production of two exotoxins: TcdA and TcdB. Moreover, for severe forms of disease, the spectrum of diseases caused by C. difficile has also been correlated to the levels of toxins that are produced during host infection. This observation strengthened the idea that the regulation of toxin synthesis is an important part of C. difficile pathogenesis. This review summarizes our current knowledge about the regulators and sigma factors that have been reported to control toxin gene expression in response to several environmental signals and stresses, including the availability of certain carbon sources and amino acids, or to signaling molecules, such as the autoinducing peptides of quorum sensing systems. The overlapping regulation of key metabolic pathways and toxin synthesis strongly suggests that toxin production is a complex response that is triggered by bacteria in response to particular states of nutrient availability during infection. PMID:27187475

  20. Diagnosis and Fault-tolerant Control for Ship Station Keeping

    DEFF Research Database (Denmark)

    Blanke, Mogens

    2005-01-01

    design for systems of high complexity, and also analyse the cases of cascaded or multiple faults. The paper takes as example a ship with two CP propellers, rudders and a bow thruster as actuators, and instrumentation with a suite of global position sensors, inertial navigation units and conventional gyro...... units to provide ship motion information. A salient feature of the design mehod is the ability to analyse cases where faults have occurrred and easily determine where in the faulty system diagnosability and controlability are retained....

  1. Fault Tolerant Controller Design for a Faulty UAV Using Fuzzy Modeling Approach

    Directory of Open Access Journals (Sweden)

    Moshu Qian

    2016-01-01

    Full Text Available We address a fault tolerant control (FTC issue about an unmanned aerial vehicle (UAV under possible simultaneous actuator saturation and faults occurrence. Firstly, the Takagi-Sugeno fuzzy models representing nonlinear flight control systems (FCS for an UAV with unknown disturbances and actuator saturation are established. Then, a normal H-infinity tracking controller is presented using an online estimator, which is introduced to weaken the saturation effect. Based on the normal tracking controller, we propose an adaptive fault tolerant tracking controller (FTTC to solve actuator loss of effectiveness (LOE fault problem. Compared with previous work, this approach developed in our research need not rely on any fault diagnosis unit and is easily applied in engineering. Finally, these results in simulation indicate the efficiency of our presented FTC scheme.

  2. Fault Tolerant and Optimal Control of Wind Turbines with Distributed High-Speed Generators

    Directory of Open Access Journals (Sweden)

    Urs Giger

    2017-01-01

    Full Text Available In this paper, the control scheme of a distributed high-speed generator system with a total amount of 12 generators and nominal generator speed of 7000 min − 1 is studied. Specifically, a fault tolerant control (FTC scheme is proposed to keep the turbine in operation in the presence of up to four simultaneous generator faults. The proposed controller structure consists of two layers: The upper layer is the baseline controller, which is separated into a partial load region with the generator torque as an actuating signal and the full-load operation region with the collective pitch angle as the other actuating signal. In addition, the lower layer is responsible for the fault diagnosis and FTC characteristics of the distributed generator drive train. The fault reconstruction and fault tolerant control strategy are tested in simulations with several actuator faults of different types.

  3. Fault-tolerant Control of Inverter-fed Induction Motor Drives

    DEFF Research Database (Denmark)

    Thybo, C.

    The main purpose of this work was to investigate how fault-tolerant control (FTC) could be included in the control scheme of frequency converter fed induction motor applications. This was approached by identifying the potential failure modes for which fault tolerant control should be applied...... a current sensor fault, by switching to a closed loop scalar controller, was analysed. The main contributions of this work are · An investigation of the potential failure modes of inverter fed induction motor drives. · An extension of the FTC development cycle, to include economical cost-benefit analysis....... A description of the different frequency converter components, including models of the inverter, sensors and controllers was given, followed by a fault mode and effect analysis, which points out the potential fault modes of the design. Among the listed fault modes, two were found to be of particular practical...

  4. Fault-tolerant Control of Inverter-fed Induction Motor Drives

    DEFF Research Database (Denmark)

    Thybo, C.

    The main purpose of this work was to investigate how fault-tolerant control (FTC) could be included in the control scheme of frequency converter fed induction motor applications. This was approached by identifying the potential failure modes for which fault tolerant control should be applied...... a current sensor fault, by switching to a closed loop scalar controller, was analysed. The main contributions of this work are · An investigation of the potential failure modes of inverter fed induction motor drives. · An extension of the FTC development cycle, to include economical cost-benefit analysis....... A description of the different frequency converter components, including models of the inverter, sensors and controllers was given, followed by a fault mode and effect analysis, which points out the potential fault modes of the design. Among the listed fault modes, two were found to be of particular practical...

  5. Satisfactory fault-tolerant controller design for uncertain systems subject to actuator faults

    Institute of Scientific and Technical Information of China (English)

    Zhang Dengfeng; Su Hongye; Wang Zhiquan

    2008-01-01

    Based on satisfactory control strategy, a new method for robust passive fault tolerant controller is proposed for a class of uncertain discrete-time systems subject to actuator faults. The state-feedback gain matrix is calculated by linear matrix inequality (LMI) technique. The designed controller guarantees that the closed-loop system meets the pre-specified consistent constraints on circular pole index and steady-state variance index simultaneously for normal case and possible actuator fault case. The consistency of the performance indices is discussed. Furthermore, with the mentioned indices constraints, a solution is obtained by convex optimal technique for the robust satisfactory fault-tolerant controller with optimal control-cost.

  6. Photosynthesis Control: An underrated short-term regulatory mechanism essential for plant viability.

    Science.gov (United States)

    Colombo, Monica; Suorsa, Marjaana; Rossi, Fabio; Ferrari, Roberto; Tadini, Luca; Barbato, Roberto; Pesaresi, Paolo

    2016-01-01

    Regulation of photosynthetic electron transport provides efficient performance of oxygenic photosynthesis in plants. During the last 15 years, the molecular bases of various photosynthesis short-term regulatory processes have been elucidated, however the wild type-like phenotypes of mutants lacking of State Transitions, Non Photochemical Quenching, or Cyclic Electron Transport, when grown under constant light conditions, have also raised doubts about the acclimatory significance of these short-regulatory mechanisms on plant performance. Interestingly, recent studies performed by growing wild type and mutant plants under field conditions revealed a prominent role of State Transitions and Non Photochemical Quenching on plant fitness, with almost no effect on vegetative plant growth. Conversely, the analysis of plants lacking the regulation of electron transport by the cytochrome b6f complex, also known as Photosynthesis Control, revealed the fundamental role of this regulatory mechanism in the survival of young, developing seedlings under fluctuating light conditions.

  7. Emerging Technologioes in Instrumentation and Controls and Their Potential Regulatory Implications for Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Korsah, Kofi [ORNL; Bobrek, Miljko [ORNL; Cetiner, Mustafa Sacit [ORNL; Ewing, Paul D [ORNL; Holcomb, David Eugene [ORNL; Howlader, Mostofa [ORNL; Killough, Stephen M [ORNL; Kisner, Roger A [ORNL; Loebl, Andy [ORNL; Moore, Michael Roy [ORNL; Muhlheim, Michael David [ORNL; Mullens, James Allen [ORNL; Shourbaji, Ayman A [ORNL; Wilson, Thomas L [ORNL

    2009-01-01

    This paper provides a summary of eight instrumentation and control (&C) technology areas, with applications in nuclear power plants (NPPs), that were the focus of a recent study performed by Oak Ridge National Laboratory (ORNL) for the Nuclear Regulatory Commission (NRC.) The state of the technology s application in NPPs, along with potential regulatory impact(s), are discussed. The technology focus areas are: (1) sensors and measurement systems, (2) communications media and networking, (3) microprocessors and other integrated circuits, (4) computational platforms, (5) surveillance, diagnostics, and prognostics, (6) human-system interactions, (7) high-integrity software, and (8) I&C architectures in new plants. The regulatory implications of these focus areas with regard to their application in NPPs are also discussed.

  8. Geometric control of ciliated band regulatory states in the sea urchin embryo.

    Science.gov (United States)

    Barsi, Julius C; Li, Enhu; Davidson, Eric H

    2015-03-01

    The trapezoidal ciliated band (CB) of the postgastrular sea urchin embryo surrounds the oral ectoderm, separating it from adjacent embryonic territories. Once differentiated, the CB is composed of densely arranged cells bearing long cilia that endow the larva with locomotion and feeding capability. The spatial pattern from which the CB will arise is first evidenced during pregastrular stages by expression of the pioneer gene onecut. Immediately after gastrulation, the CB consists of four separate regulatory state domains, each of which expresses a unique set of transcription factors: (1) the oral apical CB, located within the apical neurogenic field; (2) the animal lateral CB, which bilaterally separates the oral from aboral ectoderm; (3) the vegetal lateral CB, which bilaterally serves as signaling centers; and (4) the vegetal oral CB, which delineates the boundary with the underlying endoderm. Remarkably, almost all of the regulatory genes specifically expressed within these domains are downregulated by interference with SoxB1 expression, implying their common activation by this factor. Here, we show how the boundaries of the CB subdomains are established, and thus ascertain the design principle by which the geometry of this unique and complex regulatory state pattern is genomically controlled. Each of these boundaries, on either side of the CB, is defined by spatially confined transcriptional repressors, the products of regulatory genes operating across the border of each subdomain. In total this requires deployment of about ten different repressors, which we identify in this work, thus exemplifying the complexity of information required for spatial regulatory organization during embryogenesis.

  9. Piloted Simulator Evaluation Results of New Fault-Tolerant Flight Control Algorithm

    NARCIS (Netherlands)

    Lombaerts, T.J.J.; Smaili, M.H.; Stroosma, O.; Chu, Q.P.; Mulder, J.A.; Joosten, D.A.

    2010-01-01

    A high fidelity aircraft simulation model, reconstructed using the Digital Flight Data Recorder (DFDR) of the 1992 Amsterdam Bijlmermeer aircraft accident (Flight 1862), has been used to evaluate a new Fault-Tolerant Flight Control Algorithm in an online piloted evaluation. This paper focuses on the

  10. Active and Passive Fault-Tolerant LPV Control of Wind Turbines

    DEFF Research Database (Denmark)

    Sloth, Christoffer; Esbensen, Thomas; Stoustrup, Jakob

    2010-01-01

    This paper addresses the design and comparison of active and passive fault-tolerant linear parameter-varying (LPV) controllers for wind turbines. The considered wind turbine plant model is characterized by parameter variations along the nominal operating trajectory and includes a model of an inci...

  11. Ship Propulsion System as a Benchmark for Fault-Tolerant Control

    DEFF Research Database (Denmark)

    Izadi-Zamanabadi, Roozbeh; Blanke, M.

    1998-01-01

    -tolerant control is a fairly new area. The paper presents a ship propulsion system as a benchmark that should be useful as a platform for development of new ideas and comparison of methods. The benchmark has two main elements. One is development of efficient FDI algorithms, the other is analysis and implementation...

  12. Regulatory peptides and control of food intake in non-mammalian vertebrates.

    Science.gov (United States)

    Jensen, J

    2001-03-01

    The current view of the control of food intake involves a central feeding system in the hypothalamus receiving input from peripheral systems. The presence of food in the gut stimulates the release of several regulatory peptides that control gut motility and secretion. Some of these peptides also act as feedback satiety signals, responsible for termination of a meal. Among the regulatory peptides suggested as peripheral satiety signals are cholecystokinin and gastrin releasing peptide. A more long-term peripheral regulation of food intake has also been postulated and leptin has been suggested as a regulator of food intake. Several regulatory peptides mediate orexigenic or anorexigenic effects in the central feeding system. Neuropeptide Y and galanin both act centrally and stimulate the intake of food, while corticotropin releasing factor reduces food intake. At present, most information about the regulation of food intake is gained from mammalian studies and these findings are used as a base for a discussion on the current knowledge of how regulatory peptides control appetite in non-mammalian vertebrates.

  13. Sliding mode based fault detection, reconstruction and fault tolerant control scheme for motor systems.

    Science.gov (United States)

    Mekki, Hemza; Benzineb, Omar; Boukhetala, Djamel; Tadjine, Mohamed; Benbouzid, Mohamed

    2015-07-01

    The fault-tolerant control problem belongs to the domain of complex control systems in which inter-control-disciplinary information and expertise are required. This paper proposes an improved faults detection, reconstruction and fault-tolerant control (FTC) scheme for motor systems (MS) with typical faults. For this purpose, a sliding mode controller (SMC) with an integral sliding surface is adopted. This controller can make the output of system to track the desired position reference signal in finite-time and obtain a better dynamic response and anti-disturbance performance. But this controller cannot deal directly with total system failures. However an appropriate combination of the adopted SMC and sliding mode observer (SMO), later it is designed to on-line detect and reconstruct the faults and also to give a sensorless control strategy which can achieve tolerance to a wide class of total additive failures. The closed-loop stability is proved, using the Lyapunov stability theory. Simulation results in healthy and faulty conditions confirm the reliability of the suggested framework.

  14. Sliding mode fault detection and fault-tolerant control of smart dampers in semi-active control of building structures

    Science.gov (United States)

    Yeganeh Fallah, Arash; Taghikhany, Touraj

    2015-12-01

    Recent decades have witnessed much interest in the application of active and semi-active control strategies for seismic protection of civil infrastructures. However, the reliability of these systems is still in doubt as there remains the possibility of malfunctioning of their critical components (i.e. actuators and sensors) during an earthquake. This paper focuses on the application of the sliding mode method due to the inherent robustness of its fault detection observer and fault-tolerant control. The robust sliding mode observer estimates the state of the system and reconstructs the actuators’ faults which are used for calculating a fault distribution matrix. Then the fault-tolerant sliding mode controller reconfigures itself by the fault distribution matrix and accommodates the fault effect on the system. Numerical simulation of a three-story structure with magneto-rheological dampers demonstrates the effectiveness of the proposed fault-tolerant control system. It was shown that the fault-tolerant control system maintains the performance of the structure at an acceptable level in the post-fault case.

  15. The SIFT computer and its development. [Software Implemented Fault Tolerance for aircraft control

    Science.gov (United States)

    Goldberg, J.

    1981-01-01

    Software Implemented Fault Tolerance (SIFT) is an aircraft control computer designed to allow failure probability of less than 10 to the -10th/hour. The system is based on advanced fault-tolerance computing and validation methodology. Since confirmation of reliability by observation is essentially impossible, system reliability is estimated by a Markov model. A mathematical proof is used to justify the validity of the Markov model. System design is represented by a hierarchy of abstract models, and the design proof comprises mathematical proofs that each model is, in fact, an elaboration of the next more abstract model.

  16. Distributed Fault-Tolerant Control of Networked Uncertain Euler-Lagrange Systems Under Actuator Faults.

    Science.gov (United States)

    Chen, Gang; Song, Yongduan; Lewis, Frank L

    2016-05-03

    This paper investigates the distributed fault-tolerant control problem of networked Euler-Lagrange systems with actuator and communication link faults. An adaptive fault-tolerant cooperative control scheme is proposed to achieve the coordinated tracking control of networked uncertain Lagrange systems on a general directed communication topology, which contains a spanning tree with the root node being the active target system. The proposed algorithm is capable of compensating for the actuator bias fault, the partial loss of effectiveness actuation fault, the communication link fault, the model uncertainty, and the external disturbance simultaneously. The control scheme does not use any fault detection and isolation mechanism to detect, separate, and identify the actuator faults online, which largely reduces the online computation and expedites the responsiveness of the controller. To validate the effectiveness of the proposed method, a test-bed of multiple robot-arm cooperative control system is developed for real-time verification. Experiments on the networked robot-arms are conduced and the results confirm the benefits and the effectiveness of the proposed distributed fault-tolerant control algorithms.

  17. Robust Fault-Tolerant Control for Uncertain Networked Control Systems with State-Delay and Random Data Packet Dropout

    Directory of Open Access Journals (Sweden)

    Xiaomei Qi

    2012-01-01

    Full Text Available A robust fault-tolerant controller design problem for networked control system (NCS with random packet dropout in both sensor-to-controller link and controller-to-actuator link is investigated. A novel stochastic NCS model with state-delay, model uncertainty, disturbance, probabilistic sensor failure, and actuator failure is proposed. The random packet dropout, sensor failures, and actuator failures are characterized by a binary random variable. The sufficient condition for asymptotical mean-square stability of NCS is derived and the closed-loop NCS satisfies H∞ performance constraints caused by the random packet dropout and disturbance. The fault-tolerant controller is designed by solving a linear matrix inequality. A numerical example is presented to illustrate the effectiveness of the proposed method.

  18. Control of Pratylenchus brachyurus with Selected Nonfumigant Nematicides on a Tolerant and a Sensitive Soybean Cultivar.

    Science.gov (United States)

    Koenning, S R; Schmitt, D P

    1987-10-01

    A field study was conducted to evaluate soybean cultivar sensitivity to Pratylenchus brachyurus and selected nonfumigant nematicides for control of this nematode. 'Essex', a tolerant cultivar, yielded more than 'Forrest' , a sensitive cultivar, in an infested field. Plots treated with aldicarb, carbofuran, and fenamiphos had fewer nematodes 40 days after planting than nontreated plots. Plots planted with Forrest and treated with carbofuran had a greater yield than the untreated controls.

  19. A combinatorial regulatory signature controls terminal differentiation of the dopaminergic nervous system in C. elegans.

    Science.gov (United States)

    Doitsidou, Maria; Flames, Nuria; Topalidou, Irini; Abe, Namiko; Felton, Terry; Remesal, Laura; Popovitchenko, Tatiana; Mann, Richard; Chalfie, Martin; Hobert, Oliver

    2013-06-15

    Terminal differentiation programs in the nervous system are encoded by cis-regulatory elements that control the expression of terminal features of individual neuron types. We decoded the regulatory information that controls the expression of five enzymes and transporters that define the terminal identity of all eight dopaminergic neurons in the nervous system of the Caenorhabditis elegans hermaphrodite. We show that the tightly coordinated, robust expression of these dopaminergic enzymes and transporters ("dopamine pathway") is ensured through a combinatorial cis-regulatory signature that is shared by all dopamine pathway genes. This signature is composed of an Ets domain-binding site, recognized by the previously described AST-1 Ets domain factor, and two distinct types of homeodomain-binding sites that act in a partially redundant manner. Through genetic screens, we identified the sole C. elegans Distalless/Dlx ortholog, ceh-43, as a factor that acts through one of the homeodomain sites to control both induction and maintenance of terminal dopaminergic fate. The second type of homeodomain site is a Pbx-type site, which is recognized in a partially redundant and neuron subtype-specific manner by two Pbx factors, ceh-20 and ceh-40, revealing novel roles of Pbx factors in the context of terminal neuron differentiation. Taken together, we revealed a specific regulatory signature and cognate, terminal selector-type transcription factors that define the entire dopaminergic nervous system of an animal. Dopaminergic neurons in the mouse olfactory bulb express a similar combinatorial transcription factor collective of Ets/Dlx/Pbx factors, suggesting deep phylogenetic conservation of dopaminergic regulatory programs.

  20. A multi-layer robust adaptive fault tolerant control system for high performance aircraft

    Science.gov (United States)

    Huo, Ying

    Modern high-performance aircraft demand advanced fault-tolerant flight control strategies. Not only the control effector failures, but the aerodynamic type failures like wing-body damages often result in substantially deteriorate performance because of low available redundancy. As a result the remaining control actuators may yield substantially lower maneuvering capabilities which do not authorize the accomplishment of the air-craft's original specified mission. The problem is to solve the control reconfiguration on available control redundancies when the mission modification is urged to save the aircraft. The proposed robust adaptive fault-tolerant control (RAFTC) system consists of a multi-layer reconfigurable flight controller architecture. It contains three layers accounting for different types and levels of failures including sensor, actuator, and fuselage damages. In case of the nominal operation with possible minor failure(s) a standard adaptive controller stands to achieve the control allocation. This is referred to as the first layer, the controller layer. The performance adjustment is accounted for in the second layer, the reference layer, whose role is to adjust the reference model in the controller design with a degraded transit performance. The upmost mission adjust is in the third layer, the mission layer, when the original mission is not feasible with greatly restricted control capabilities. The modified mission is achieved through the optimization of the command signal which guarantees the boundedness of the closed-loop signals. The main distinguishing feature of this layer is the the mission decision property based on the current available resources. The contribution of the research is the multi-layer fault-tolerant architecture that can address the complete failure scenarios and their accommodations in realities. Moreover, the emphasis is on the mission design capabilities which may guarantee the stability of the aircraft with restricted post

  1. Optimal Control of Gene Regulatory Networks with Effectiveness of Multiple Drugs: A Boolean Network Approach

    Science.gov (United States)

    Kobayashi, Koichi; Hiraishi, Kunihiko

    2013-01-01

    Developing control theory of gene regulatory networks is one of the significant topics in the field of systems biology, and it is expected to apply the obtained results to gene therapy technologies in the future. In this paper, a control method using a Boolean network (BN) is studied. A BN is widely used as a model of gene regulatory networks, and gene expression is expressed by a binary value (0 or 1). In the control problem, we assume that the concentration level of a part of genes is arbitrarily determined as the control input. However, there are cases that no gene satisfying this assumption exists, and it is important to consider structural control via external stimuli. Furthermore, these controls are realized by multiple drugs, and it is also important to consider multiple effects such as duration of effect and side effects. In this paper, we propose a BN model with two types of the control inputs and an optimal control method with duration of drug effectiveness. First, a BN model and duration of drug effectiveness are discussed. Next, the optimal control problem is formulated and is reduced to an integer linear programming problem. Finally, numerical simulations are shown. PMID:24058904

  2. Safety Verification of a Fault Tolerant Reconfigurable Autonomous Goal-Based Robotic Control System

    Science.gov (United States)

    Braman, Julia M. B.; Murray, Richard M; Wagner, David A.

    2007-01-01

    Fault tolerance and safety verification of control systems are essential for the success of autonomous robotic systems. A control architecture called Mission Data System (MDS), developed at the Jet Propulsion Laboratory, takes a goal-based control approach. In this paper, a method for converting goal network control programs into linear hybrid systems is developed. The linear hybrid system can then be verified for safety in the presence of failures using existing symbolic model checkers. An example task is simulated in MDS and successfully verified using HyTech, a symbolic model checking software for linear hybrid systems.

  3. Passive fault tolerant control of a double inverted pendulum - a case study

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik; Stoustrup, Jakob

    2005-01-01

    A passive fault tolerant control scheme is suggested, in which a nominal controller is augmented with an additional block, which guarantees stability and performance after the occurrence of a fault. The method is based on the YJBK parameterization, which requires the nominal controller to be impl...... to be implemented in observer based form. The proposed method is applied to a double inverted pendulum system, for which an H_inf controller has been designed and verified in a lab setup. In this case study, the fault is a degradation of the tacho loop....

  4. Methylphenidate blocks effort-induced depletion of regulatory control in healthy volunteers.

    Science.gov (United States)

    Sripada, Chandra; Kessler, Daniel; Jonides, John

    2014-06-01

    A recent wave of studies--more than 100 conducted over the last decade--has shown that exerting effort at controlling impulses or behavioral tendencies leaves a person depleted and less able to engage in subsequent rounds of regulation. Regulatory depletion is thought to play an important role in everyday problems (e.g., excessive spending, overeating) as well as psychiatric conditions, but its neurophysiological basis is poorly understood. Using a placebo-controlled, double-blind design, we demonstrated that the psychostimulant methylphenidate (commonly known as Ritalin), a catecholamine reuptake blocker that increases dopamine and norepinephrine at the synaptic cleft, fully blocks effort-induced depletion of regulatory control. Spectral analysis of trial-by-trial reaction times revealed specificity of methylphenidate effects on regulatory depletion in the slow-4 frequency band. This band is associated with the operation of resting-state brain networks that produce mind wandering, which raises potential connections between our results and recent brain-network-based models of control over attention.

  5. Active fault tolerant control of piecewise affine systems with reference tracking and input constraints

    DEFF Research Database (Denmark)

    Gholami, M.; Cocquempot, V.; Schiøler, H.

    2014-01-01

    An active fault tolerant control (AFTC) method is proposed for discrete-time piecewise affine (PWA) systems. Only actuator faults are considered. The AFTC framework contains a supervisory scheme, which selects a suitable controller in a set of controllers such that the stability and an acceptable...... the reference signal while the control inputs are bounded. The PFTC problem is transformed into a feasibility problem of a set of LMIs. The method is applied on a large-scale live-stock ventilation model.......An active fault tolerant control (AFTC) method is proposed for discrete-time piecewise affine (PWA) systems. Only actuator faults are considered. The AFTC framework contains a supervisory scheme, which selects a suitable controller in a set of controllers such that the stability and an acceptable...... performance of the faulty system are held. The design of the supervisory scheme is not considered here. The set of controllers is composed of a normal controller for the fault-free case, an active fault detection and isolation controller for isolation and identification of the faults, and a set of passive...

  6. Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes

    DEFF Research Database (Denmark)

    Ventre, I.; Goodman, A.L.; Vallet-Gely, I.

    2006-01-01

    S, a hybrid sensor kinase that controls the reciprocal expression of genes for type III secretion and biofilm-promoting polysaccharicles. Domain organization of LadS and the range of LadS-controlled genes suggest that it counteracts the activities of another sensor kinase, RetS. These two pathways converge...... by controlling the transcription of a small regulatory RNA, RsmZ. This work identifies a previously undescribed signal transduction network in which the activities of signal-receiving sensor kinases LadS, RetS, and GacS regulate expression of virulence genes associated with acute or chronic infection...

  7. Self-regulatory fatigue in chronic multisymptom illnesses: scale development, fatigue, and self-control.

    Science.gov (United States)

    Nes, Lise Solberg; Ehlers, Shawna L; Whipple, Mary O; Vincent, Ann

    2013-01-01

    Self-regulatory capacity involves ability to regulate thoughts, emotions, and behavior. Chronic multisymptom illnesses such as fibromyalgia and chronic fatigue syndrome are accompanied by numerous challenges, and have recently been associated with self-regulatory fatigue (SRF). Chronic multisymptom illnesses are also frequently associated with physical fatigue, and through development of a scale measuring SRF, the current study aimed to examine how SRF can be distinguished from physical fatigue. The study also sought to distinguish SRF from self-control. Two self-regulation researchers developed 30 items related to self-regulatory capacity. These items were distributed to patients (n = 296) diagnosed with chronic multisymptom illness together with validated measures of physical fatigue and self-control. A principal factor analysis was employed to examine factor structures, identify inter-item relationships, and aid in scale development. The final proposed scale consisted of 18 items measuring self-regulatory capacity (SRF-18) with cognitive, emotional, and behavioral SRF components. Internal consistency and reliability was acceptable (Cronbach's á = 0.81). The final scale was moderately correlated with self-control (r = -0.48) and highly correlated with physical fatigue (r = 0.75), although more so with emotional (r = 0.72) and mental (r = 0.65) than physical (r = 0.46) fatigue components. The current study suggests a new scale for measurement of SRF in chronic multisymptom illness. Although cross-validation studies are necessary, such a scale may contribute to a better understanding of the concept of self-regulation and the role of SRF in chronic illness. Although related to physical fatigue and self-control, the results point to SRF as a distinct construct.

  8. Backstepping Design of Adaptive Neural Fault-Tolerant Control for MIMO Nonlinear Systems.

    Science.gov (United States)

    Gao, Hui; Song, Yongduan; Wen, Changyun

    2016-08-24

    In this paper, an adaptive controller is developed for a class of multi-input and multioutput nonlinear systems with neural networks (NNs) used as a modeling tool. It is shown that all the signals in the closed-loop system with the proposed adaptive neural controller are globally uniformly bounded for any external input in L[₀,∞]. In our control design, the upper bound of the NN modeling error and the gains of external disturbance are characterized by unknown upper bounds, which is more rational to establish the stability in the adaptive NN control. Filter-based modification terms are used in the update laws of unknown parameters to improve the transient performance. Finally, fault-tolerant control is developed to accommodate actuator failure. An illustrative example applying the adaptive controller to control a rigid robot arm shows the validation of the proposed controller.

  9. Fault-tolerant control for current sensors of doubly fed induction generators based on an improved fault detection method

    DEFF Research Database (Denmark)

    Li, Hui; Yang, Chao; Hu, Yaogang

    2014-01-01

    Fault-tolerant control of current sensors is studied in this paper to improve the reliability of a doubly fed induction generator (DFIG). A fault-tolerant control system of current sensors is presented for the DFIG, which consists of a new current observer and an improved current sensor fault...... detection algorithm, and fault-tolerant control system are investigated by simulation. The results indicate that the outputs of the observer and the sensor are highly coherent. The fault detection algorithm can efficiently detect both soft and hard faults in current sensors, and the fault-tolerant control...... system can effectively tolerate both types of faults. © 2013 Published by Elsevier Ltd. All rights reserved....

  10. Optimal fault-tolerant control strategy of a solid oxide fuel cell system

    Science.gov (United States)

    Wu, Xiaojuan; Gao, Danhui

    2017-10-01

    For solid oxide fuel cell (SOFC) development, load tracking, heat management, air excess ratio constraint, high efficiency, low cost and fault diagnosis are six key issues. However, no literature studies the control techniques combining optimization and fault diagnosis for the SOFC system. An optimal fault-tolerant control strategy is presented in this paper, which involves four parts: a fault diagnosis module, a switching module, two backup optimizers and a controller loop. The fault diagnosis part is presented to identify the SOFC current fault type, and the switching module is used to select the appropriate backup optimizer based on the diagnosis result. NSGA-II and TOPSIS are employed to design the two backup optimizers under normal and air compressor fault states. PID algorithm is proposed to design the control loop, which includes a power tracking controller, an anode inlet temperature controller, a cathode inlet temperature controller and an air excess ratio controller. The simulation results show the proposed optimal fault-tolerant control method can track the power, temperature and air excess ratio at the desired values, simultaneously achieving the maximum efficiency and the minimum unit cost in the case of SOFC normal and even in the air compressor fault.

  11. Dynamic Output Feedback Based Active Decentralized Fault-Tolerant Control for Reconfigurable Manipulator with Concurrent Failures

    Directory of Open Access Journals (Sweden)

    Yuanchun Li

    2015-01-01

    Full Text Available The goal of this paper is to describe an active decentralized fault-tolerant control (ADFTC strategy based on dynamic output feedback for reconfigurable manipulators with concurrent actuator and sensor failures. Consider each joint module of the reconfigurable manipulator as a subsystem, and treat the fault as the unknown input of the subsystem. Firstly, by virtue of linear matrix inequality (LMI technique, the decentralized proportional-integral observer (DPIO is designed to estimate and compensate the sensor fault online; hereafter, the compensated system model could be derived. Then, the actuator fault is estimated similarly by another DPIO using LMI as well, and the sufficient condition of the existence of H∞ fault-tolerant controller in the dynamic output feedback is presented for the compensated system model. Furthermore, the dynamic output feedback controller is presented based on the estimation of actuator fault to realize active fault-tolerant control. Finally, two 3-DOF reconfigurable manipulators with different configurations are employed to verify the effectiveness of the proposed scheme in simulation. The main advantages of the proposed scheme lie in that it can handle the concurrent faults act on the actuator and sensor on the same joint module, as well as there is no requirement of fault detection and isolation process; moreover, it is more feasible to the modularity of the reconfigurable manipulator.

  12. Design of passive fault-tolerant flight controller against actuator failures

    Directory of Open Access Journals (Sweden)

    Xiang Yu

    2015-02-01

    Full Text Available The problem of designing passive fault-tolerant flight controller is addressed when the normal and faulty cases are prescribed. First of all, the considered fault and fault-free cases are formed by polytopes. As considering that the safety of a post-fault system is directly related to the maximum values of physical variables in the system, peak-to-peak gain is selected to represent the relationships among the amplitudes of actuator outputs, system outputs, and reference commands. Based on the parameter dependent Lyapunov and slack methods, the passive fault-tolerant flight controllers in the absence/presence of system uncertainty for actuator failure cases are designed, respectively. Case studies of an airplane under actuator failures are carried out to validate the effectiveness of the proposed approach.

  13. Design of passive fault-tolerant flight controller against actuator failures

    Institute of Scientific and Technical Information of China (English)

    Yu Xiang; Zhang Youmin

    2015-01-01

    The problem of designing passive fault-tolerant flight controller is addressed when the normal and faulty cases are prescribed. First of all, the considered fault and fault-free cases are formed by polytopes. As considering that the safety of a post-fault system is directly related to the maximum values of physical variables in the system, peak-to-peak gain is selected to represent the relationships among the amplitudes of actuator outputs, system outputs, and reference com-mands. Based on the parameter dependent Lyapunov and slack methods, the passive fault-tolerant flight controllers in the absence/presence of system uncertainty for actuator failure cases are designed, respectively. Case studies of an airplane under actuator failures are carried out to validate the effectiveness of the proposed approach.

  14. H infinity Integrated Fault Estimation and Fault Tolerant Control of Discrete-time Piecewise Linear Systems

    DEFF Research Database (Denmark)

    Tabatabaeipour, Seyed Mojtaba; Bak, Thomas

    2012-01-01

    In this paper we consider the problem of fault estimation and accommodation for discrete time piecewise linear systems. A robust fault estimator is designed to estimate the fault such that the estimation error converges to zero and H∞ performance of the fault estimation is minimized. Then......, the estimate of fault is used to compensate for the effect of the fault. Hence, using the estimate of fault, a fault tolerant controller using a piecewise linear static output feedback is designed such that it stabilizes the system and provides an upper bound on the H∞ performance of the faulty system....... Sufficient conditions for the existence of robust fault estimator and fault tolerant controller are derived in terms of linear matrix inequalities. Upper bounds on the H∞ performance can be minimized by solving convex optimization problems with linear matrix inequality constraints. The efficiency...

  15. Adaptive sensor-fault tolerant control for a class of multivariable uncertain nonlinear systems.

    Science.gov (United States)

    Khebbache, Hicham; Tadjine, Mohamed; Labiod, Salim; Boulkroune, Abdesselem

    2015-03-01

    This paper deals with the active fault tolerant control (AFTC) problem for a class of multiple-input multiple-output (MIMO) uncertain nonlinear systems subject to sensor faults and external disturbances. The proposed AFTC method can tolerate three additive (bias, drift and loss of accuracy) and one multiplicative (loss of effectiveness) sensor faults. By employing backstepping technique, a novel adaptive backstepping-based AFTC scheme is developed using the fact that sensor faults and system uncertainties (including external disturbances and unexpected nonlinear functions caused by sensor faults) can be on-line estimated and compensated via robust adaptive schemes. The stability analysis of the closed-loop system is rigorously proven using a Lyapunov approach. The effectiveness of the proposed controller is illustrated by two simulation examples.

  16. Systematic Fault Tolerant Control Based on Adaptive Thau Observer Estimation for Quadrotor Uavs

    Directory of Open Access Journals (Sweden)

    Cen Zhaohui

    2015-03-01

    Full Text Available A systematic fault tolerant control (FTC scheme based on fault estimation for a quadrotor actuator, which integrates normal control, active and passive FTC and fault parking is proposed in this paper. Firstly, an adaptive Thau observer (ATO is presented to estimate the quadrotor rotor fault magnitudes, and then faults with different magnitudes and time-varying natures are rated into corresponding fault severity levels based on the pre-defined fault-tolerant boundaries. Secondly, a systematic FTC strategy which can coordinate various FTC methods is designed to compensate for failures depending on the fault types and severity levels. Unlike former stand-alone passive FTC or active FTC, our proposed FTC scheme can compensate for faults in a way of condition-based maintenance (CBM, and especially consider the fatal failures that traditional FTC techniques cannot accommodate to avoid the crashing of UAVs. Finally, various simulations are carried out to show the performance and effectiveness of the proposed method.

  17. An adaptive fuzzy design for fault-tolerant control of MIMO nonlinear uncertain systems

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    This paper presents a novel control method for accommodating actuator faults in a class of multiple-input multiple-output (MIMO) nonlinear uncertain systems.The designed control scheme can tolerate both the time-varying lock-in-place and loss of effectiveness actuator faults.In each subsystem of the considered MIMO system,the controller is obtained from a backstepping procedure;an adaptive fuzzy approximator with minimal learning parameterization is employed to approximate the package of unknown nonlinear f...

  18. ONLINE MODEL AND ACTUATOR FAULT TOLERANT CONTROL FOR AUTONOMOUS MOBILE ROBOT

    Institute of Scientific and Technical Information of China (English)

    SONG Qi; JIANG Zhe; HAN Jianda

    2007-01-01

    A novel fault-tolerant adaptive control methodology against the actuator faults is proposed.The actuator effectiveness factors (AEFs) are introduced to denote the healthy of actuator, and the unscented Kalman filter (UKF) is employed for online estimation of both the motion states and the AEFs of mobile robot. A square root version of the UKF is introduced to improve efficiency and numerical stability. Using the information from the UKF, the reconfigurable controller is designed automatically based on an enhancement inverse dynamic control (IDC) methodology. The experiment on a 3-DOF omni-directional mobile robot is performed, and the effectiveness of the proposed method is demonstrated.

  19. Novel active fault-tolerant control scheme and its application to a double inverted pendulum system

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    On the basis of the gain-scheduled H∞ design strategy,a novel active fault-tolerant control scheme is proposed.Under the assumption that the effects of faults on the state-space matrices of systems can be of affine parameter dependence,a reconfigurable robust H∞ linear parameter varying controller is developed.The designed controller is a function of the fault effect factors that can be derived online by using a well-trained neural network.To demonstrate the effectiveness of the proposed method,a double inverted pendulum system,with a fault in the motor tachometer loop,is considered.

  20. Imprecise Computation Based Real-time Fault Tolerant Implementation for Model Predictive Control

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Model predictive control (MPC) could not be deployed in real-time control systems for its computation time is not well defined. A real-time fault tolerant implementation algorithm based on imprecise computation is proposed for MPC,according to the solving process of quadratic programming (QP) problem. In this algorithm, system stability is guaranteed even when computation resource is not enough to finish optimization completely. By this kind of graceful degradation, the behavior of real-time control systems is still predictable and determinate. The algorithm is demonstrated by experiments on servomotor, and the simulation results show its effectiveness.

  1. Distributed Interplanetary Delay/Disruption Tolerant Network (DTN) Monitor and Control System

    Science.gov (United States)

    Wang, Shin-Ywan

    2012-01-01

    The main purpose of Distributed interplanetary Delay Tolerant Network Monitor and Control System as a DTN system network management implementation in JPL is defined to provide methods and tools that can monitor the DTN operation status, detect and resolve DTN operation failures in some automated style while either space network or some heterogeneous network is infused with DTN capability. In this paper, "DTN Monitor and Control system in Deep Space Network (DSN)" exemplifies a case how DTN Monitor and Control system can be adapted into a space network as it is DTN enabled.

  2. A novel fault tolerant permanent magnet synchronous motor with improved optimal torque control for aerospace application

    Directory of Open Access Journals (Sweden)

    Guo Hong

    2015-04-01

    Full Text Available Improving fault tolerant performance of permanent magnet synchronous motor has always been the central issue of the electrically supplied actuator for aerospace application. In this paper, a novel fault tolerant permanent magnet synchronous motor is proposed, which is characterized by two stators and two rotors on the same shaft with a circumferential displacement of mechanical angle of 4.5°. It helps to reduce the cogging torque. Each segment of the stator and the rotor can be considered as an 8-pole/10-slot five-phase permanent magnet synchronous motor with concentrated, single-layer and alternate teeth wound winding, which enhance the fault isolation capacity of the motor. Furthermore, the motor has high phase inductance to restrain the short-circuit current. In addition, an improved optimal torque control strategy is proposed to make the motor work well under the open-circuit fault and short-circuit fault conditions. Simulation and experiment results show that the proposed fault tolerant motor system has excellent fault tolerant capacity, which is able to operate continuously under the third open-circuit fault and second short-circuit fault condition without system performance degradation, which was not available earlier.

  3. An LMI Method to Robust Iterative Learning Fault-tolerant Guaranteed Cost Control for Batch Processes

    Institute of Scientific and Technical Information of China (English)

    WANG Limin; CHEN Xi; GAO Furong

    2013-01-01

    Based on an equivalent two-dimensional Fornasini-Marchsini model for a batch process in industry,a closed-loop robust iterative learning fault-tolerant guaranteed cost control scheme is proposed for batch processes with actuator failures.This paper introduces relevant concepts of the fault-tolerant guaranteed cost control and formulates the robust iterative learning reliable guaranteed cost controller (ILRGCC).A significant advantage is that the proposed ILRGCC design method can be used for on-line optimization against batch-to-batch process uncertainties to realize robust tracking of set-point trajectory in time and batch-to-batch sequences.For the convenience of implementation,only measured output errors of current and previous cycles are used to design a synthetic controller for iterative learning control,consisting of dynamic output feedback plus feed-forward control.The proposed controller can not only guarantee the closed-loop convergency along time and cycle sequences but also satisfy the H∞ performance level and a cost function with upper bounds for all admissible uncertainties and any actuator failures.Sufficient conditions for the controller solution are derived in terms of linear matrix inequalities (LMIs),and design procedures,which formulate a convex optimization problem with LMI constraints,are presented.An example of injection molding is given to illustrate the effectiveness and advantages of the ILRGCC design approach.

  4. Robust and Active Fault-tolerant Control for a Class of Nonlinear Uncertain Systems

    Institute of Scientific and Technical Information of China (English)

    You-Qing Wang; Dong-Hua Zhou; Li-Heng Liu

    2006-01-01

    A novel integrated design strategy for robust fault diagnosis and fault-tolerant control (FTC) of a class of nonlinear uncertain systems is proposed. The uncertainties considered in this paper are more general than those in other existing works, and faults are described in a new formulation. It is proven that the states of a closed-loop system converge asymptotically to zero even if there are uncertainties and faults in a system. Simulation results on a simple pendulum are presented for illustration.

  5. Reconfigurable fault-tolerant multiprocessor system for real-time control

    Energy Technology Data Exchange (ETDEWEB)

    Kao, M.L.

    1986-01-01

    Real-time control applications place stringent constraints in computers controlling them since the failure of a computer could result in costly damages and even loss of human lives. Fault-tolerant computers, therefore, have been always in high demand in critical avionic and aerospace applications. However, the use of redundancy techniques to achieve fault tolerance in industrial applications has only recently become feasible due to the rapid decrease in cost and increase in performance of microprocessors. As more and more robots are being built to replace human beings in dangerous and difficult tasks, the need for a reliable computer for robotics control increases. This need, in particular, motivated the research described in this dissertation - the design and implementation of a reconfigurable fault-tolerant multiprocessor system (the FREMP system). The FREMP system consists of four processing units (PUs) and three common parallel buses. Each PU is a combination of an Intel 86/30 single board computer and a custom fault detection/masking circuit board (FDM board). A hardware/software combined scheme was devised to detect faults and correct errors. This scheme has shown to be more efficient than software voting while maintaining the flexibility of software approaches. Time-frame scheduling was adopted to schedule tasks for execution.

  6. The Importance of the Nurse Cells and Regulatory Cells in the Control of T Lymphocyte Responses

    Directory of Open Access Journals (Sweden)

    María Guadalupe Reyes García

    2013-01-01

    Full Text Available T lymphocytes from the immune system are bone marrow-derived cells whose development and activities are carefully supervised by two sets of accessory cells. In the thymus, the immature young T lymphocytes are engulfed by epithelial “nurse cells” and retained in vacuoles, where most of them (95% are negatively selected and removed when they have an incomplete development or express high affinity autoreactive receptors. The mature T lymphocytes that survive to this selection process leave the thymus and are controlled in the periphery by another subpopulation of accessory cells called “regulatory cells,” which reduce any excessive immune response and the risk of collateral injuries to healthy tissues. By different times and procedures, nurse cells and regulatory cells control both the development and the functions of T lymphocyte subpopulations. Disorders in the T lymphocytes development and migration have been observed in some parasitic diseases, which disrupt the thymic microenvironment of nurse cells. In other cases, parasites stimulate rather than depress the functions of regulatory T cells decreasing T-mediated host damages. This paper is a short review regarding some features of these accessory cells and their main interactions with T immature and mature lymphocytes. The modulatory role that neurotransmitters and hormones play in these interactions is also revised.

  7. Regulatory Initiatives for Control and Release of Technologically Enhanced Naturally-Occurring Radioactive Materials

    Energy Technology Data Exchange (ETDEWEB)

    Egidi, P.V.

    1999-03-02

    Current drafts of proposed standards and suggested State regulations for control and release of technologically-enhanced naturally-occurring radioactive material (TENORM), and standards for release of volumetrically-contaminated material in the US are reviewed. These are compared to the recommendations of the International Atomic Energy Association (IAEA) Safety Series and the European Commission (EC) proposals. Past regulatory efforts with respect to TENORM in the US dealt primarily with oil-field related wastes. Currently, nine states (AK, GA, LA, MS, NM, OH, OR SC, TX) have specific regulations pertaining to TENORM, mostly based on uranium mill tailings cleanup criteria. The new US proposals are dose- or risk-based, as are the IAEA and EC recommendations, and are grounded in the linear no threshold hypothesis (LNT). TENORM wastes involve extremely large volumes, particularly scrap metal and mine wastes. Costs to control and dispose of these wastes can be considerable. The current debate over the validity of LNT at low doses and low dose rates is particularly germane to this discussion. Most standards setting organizations and regulatory agencies base their recommendations on the LNT. The US Environmental Protection Agency has released a draft Federal Guidance Report that recommends calculating health risks from low-level exposure to radionuclides based on the LNT. However, some scientific and professional organizations are openly questioning the validity of LNT and its basis for regulations, practices, and costs to society in general. It is not clear at this time how a non-linear regulatory scheme would be implemented.

  8. A robust adaptive nonlinear fault-tolerant controller via norm estimation for reusable launch vehicles

    Science.gov (United States)

    Hu, Chaofang; Gao, Zhifei; Ren, Yanli; Liu, Yunbing

    2016-11-01

    In this paper, a reusable launch vehicle (RLV) attitude control problem with actuator faults is addressed via the robust adaptive nonlinear fault-tolerant control (FTC) with norm estimation. Firstly, the accurate tracking task of attitude angles in the presence of parameter uncertainties and external disturbances is considered. A fault-free controller is proposed using dynamic surface control (DSC) combined with fuzzy adaptive approach. Furthermore, the minimal learning parameter strategy via norm estimation technique is introduced to reduce the multi-parameter adaptive computation burden of fuzzy approximation of the lump uncertainties. Secondly, a compensation controller is designed to handle the partial loss fault of actuator effectiveness. The unknown maximum eigenvalue of actuator efficiency loss factors is estimated online. Moreover, stability analysis guarantees that all signals of the closed-loop control system are semi-global uniformly ultimately bounded. Finally, illustrative simulations show the effectiveness of the proposed method.

  9. Guaranteed Cost Active Fault-tolerant Control of Networked Control System with Packet Dropout and Transmission Delay

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yuan Luo; Mei-Jie Shang; Cai-Lian Chen; Xin-Ping Guan

    2010-01-01

    The problem of guaranteed cost active fault-tolerant controller (AFTC) design for networked control systems (NCSs)with both packet dropout and transmission delay is studied in this paper.Considering the packet dropout and transmission delay,a piecewise constant controller is adopted.With a guaranteed cost function,optimal controllers whose number is equal to the number of actuators are designed,and the design process is formulated as a convex optimal problem that can be solved by existing software.The control strategy is proposed as follows:when actuator failures appear,the fault detection and isolation unit sends out the information to the controller choosing strategy,and then the optimal stabilizing controller with the smallest guaranteed cost value is chosen.Two illustrative examples are given to demonstrate the effectiveness of the proposed approach.By comparing with the existing methods,it can be seen that our method has a better performance.

  10. Fault-tolerant Control of Discrete-time LPV systems using Virtual Actuators and Sensors

    DEFF Research Database (Denmark)

    Tabatabaeipour, Mojtaba; Stoustrup, Jakob; Bak, Thomas

    2015-01-01

    This paper proposes a new fault-tolerant control (FTC) method for discrete-time linear parameter varying (LPV) systems using a reconfiguration block. The basic idea of the method is to achieve the FTC goal without re-designing the nominal controller by inserting a reconfiguration block between th....... Finally, the effectiveness of the method is demonstrated via a numerical example and stator current control of an induction motor.......This paper proposes a new fault-tolerant control (FTC) method for discrete-time linear parameter varying (LPV) systems using a reconfiguration block. The basic idea of the method is to achieve the FTC goal without re-designing the nominal controller by inserting a reconfiguration block between......, it transforms the output of the controller for the faulty system such that the stability and performance goals are preserved. Input-to-state stabilizing LPV gains of the virtual actuator and sensor are obtained by solving linear matrix inequalities (LMIs). We show that separate design of these gains guarantees...

  11. ALLIANCE: An architecture for fault tolerant, cooperative control of heterogeneous mobile robots

    Energy Technology Data Exchange (ETDEWEB)

    Parker, L.E.

    1995-02-01

    This research addresses the problem of achieving fault tolerant cooperation within small- to medium-sized teams of heterogeneous mobile robots. The author describes a novel behavior-based, fully distributed architecture, called ALLIANCE, that utilizes adaptive action selection to achieve fault tolerant cooperative control in robot missions involving loosely coupled, largely independent tasks. The robots in this architecture possess a variety of high-level functions that they can perform during a mission, and must at all times select an appropriate action based on the requirements of the mission, the activities of other robots, the current environmental conditions, and their own internal states. Since such cooperative teams often work in dynamic and unpredictable environments, the software architecture allows the team members to respond robustly and reliably to unexpected environmental changes and modifications in the robot team that may occur due to mechanical failure, the learning of new skills, or the addition or removal of robots from the team by human intervention. After presenting ALLIANCE, the author describes in detail experimental results of an implementation of this architecture on a team of physical mobile robots performing a cooperative box pushing demonstration. These experiments illustrate the ability of ALLIANCE to achieve adaptive, fault-tolerant cooperative control amidst dynamic changes in the capabilities of the robot team.

  12. A Piecewise Affine Hybrid Systems Approach to Fault Tolerant Satellite Formation Control

    DEFF Research Database (Denmark)

    Grunnet, Jacob Deleuran; Larsen, Jesper Abildgaard; Bak, Thomas

    2008-01-01

    In this paper a procedure for modelling satellite formations   including failure dynamics as a piecewise-affine hybrid system is   shown. The formulation enables recently developed methods and tools   for control and analysis of piecewise-affine systems to be applied   leading to synthesis of fault...... tolerant controllers and analysis of   the system behaviour given possible faults.  The method is   illustrated using a simple example involving two satellites trying   to reach a specific formation despite of actuator faults occurring....

  13. A Piecewise Affine Hybrid Systems Approach to Fault Tolerant Satellite Formation Control

    DEFF Research Database (Denmark)

    Grunnet, Jacob Deleuran; Larsen, Jesper Abildgaard; Bak, Thomas

    2008-01-01

    In this paper a procedure for modelling satellite formations   including failure dynamics as a piecewise-affine hybrid system is   shown. The formulation enables recently developed methods and tools   for control and analysis of piecewise-affine systems to be applied   leading to synthesis of fault...... tolerant controllers and analysis of   the system behaviour given possible faults.  The method is   illustrated using a simple example involving two satellites trying   to reach a specific formation despite of actuator faults occurring....

  14. Robust H-infinity fault-tolerant control for uncertain descriptor systems by dynamical compensators

    Institute of Scientific and Technical Information of China (English)

    Bing LIANG; Guangren DUAN

    2004-01-01

    The problem of robust H-infinity fault-tolerant control against sensor failures for a class of uncertain descriptor systems via dynamical compensators is considered.Based on H-infinity theory in descriptor systems,a sufficient condition for the existence of dynamical compensators with H-infinity fault-tolerant function is derived and expressions for the gain matrices in the compensators are presented.The dynamical compensator guarantees that the resultant colsed-loop system is admissible;furthermore,it maintains certain H-infinity norm performance in the normal condition as well as in the event of sensor failures and parameter uncertainties.A numerical example shows the effect of the proposed method.

  15. The antianginal efficacy and tolerability of controlled-release metoprolol once daily

    DEFF Research Database (Denmark)

    Egstrup, K; Gundersen, T; Härkönen, R;

    1988-01-01

    In a randomized, double-blind, cross-over study treatment with a new controlled-release (CR) preparation of metoprolol, given once daily, was compared with treatment with conventional metoprolol tablets, given twice daily, in 115 patients with stable effort angina pectoris. The patients were...... questionnaire. When all patients were analysed together there were no differences in antianginal efficacy between the two treatment regimens. However, when the group taking 200 mg daily was analysed separately better exercise tolerance was found during metoprolol CR therapy, as measured by onset of chest pain...... and ST-segment change, compared with conventional metoprolol therapy. The two formulations were well tolerated. When given once daily in a total daily dose of 100 mg, the CR preparation induced less adverse effects than the conventional tablets, 50 mg twice daily. It was concluded that the new metoprolol...

  16. An overview of the gene regulatory network controlling trichome development in the model plant, Arabidopsis

    Directory of Open Access Journals (Sweden)

    Sitakanta ePattanaik

    2014-06-01

    Full Text Available Trichomes are specialized epidermal cells located on aerial parts of plants and are associated with a wide array of biological processes. Trichomes protect plants from adverse conditions including UV light and herbivore attack and are also an important source of a number of phytochemicals. The simple unicellular trichomes of Arabidopsis serve as an excellent model to study molecular mechanism of cell differentiation and pattern formation in plants. The emerging picture suggests that the developmental process is controlled by a transcriptional network involving three major groups of transcription factors: the R2R3 MYB, basic helix-loop-helix (bHLH and WD40 repeat (WDR protein. These regulatory proteins form a trimeric activator complex that positively regulates trichome development. The single repeat R3 MYBs act as negative regulators of trichome development. They compete with the R2R3 MYBs to bind the bHLH factor and form a repressor complex. In addition to activator-repressor mechanism, a depletion mechanism may operate in parallel during trichome development. In this mechanism, the bHLH factor traps the WDR protein which results in depletion of WDR protein in neighboring cells. Consequently, the cells with high levels of bHLH and WDR proteins are developed into trichomes. A group of C2H2 zinc finger TFs has also been implicated in trichome development. Phytohormones, including gibberellins and jasmonic acid, play significant roles in this developmental process. Recently, microRNAs have been shown to be involved in trichome development. Furthermore, it has been demonstrated that the activities of the key regulatory proteins involved in trichome development are controlled by the 26S/ubiquitin proteasome system (UPS, highlighting the complexity of the regulatory network controlling this developmental process. To complement several excellent recent relevant reviews, this review focuses on the transcriptional network and hormonal interplay

  17. Combining Artificial Intelligence and Advanced Techniques in Fault-Tolerant Control

    Directory of Open Access Journals (Sweden)

    A. Vargas-Martínez

    2011-08-01

    Full Text Available We present the integration of artificial intelligence, robust, nonlinear and model reference adaptive control (MRACmethods for fault-tolerant control (FTC. We combine MRAC schemes with classical PID controllers, artificial neuralnetworks (ANNs, genetic algorithms (GAs, H∞ controls and sliding mode controls. Six different schemas areproposed: the first one is an MRAC with an artificial neural network and a PID controller whose parameters weretuned by a GA using Pattern Search Optimization. The second scheme is an MRAC controller with an H∞ control(H∞. The third scheme is an MRAC controller with a sliding mode controller (SMC. The fourth scheme is an MRACcontroller with an ANN. The fifth scheme is an MRAC controller with a PID controller optimized by a GA. Finally, thelast scheme is an MRAC classical control system. The objective of this research is to generate more powerful FTCmethods and compare the performance of above schemes under different fault conditions in sensors and actuators.An industrial heat exchanger process was the test bed for these approaches. Simulation results showed that the useof Pattern Search Optimization and ANNs improved the performance of the FTC scheme because it makes the controlsystem more robust against sensor and actuator faults.

  18. Control of porcine reproductive and respiratory syndrome (PRRS through genetic improvements in disease resistance and tolerance

    Directory of Open Access Journals (Sweden)

    Raymond eRowland

    2012-12-01

    Full Text Available Infections caused by porcine reproductive and respiratory syndrome virus (PRRSV have a severe economic impact on pig production in North America, Europe and Asia. The emergence and eventual predominance of PRRS in the 1990s are the likely result of changes in the pork industry initiated in the late 1970s, which allowed the virus to occupy a unique niche within a modern commercial production system. PRRSV infection is responsible for severe clinical disease, but can maintain a life-long subclinical infection, as well as participate in several polymicrobial syndromes. Current vaccines lessen clinical signs, but are of limited use for disease control and elimination. The relatively poor protective immunity following vaccination is a function of the virus’s capacity to generate a large degree of genetic diversity, combined with several strategies to evade innate and adaptive immune responses. In 2007, the PRRS Host Genetics consortium (PHGC was established to explore the role of host genetics as as an avenue for PRRS control. The PHGC model for PRRS incorporates the experimental infection of large numbers of growing pigs and has created the opportunity to study experimental PRRSV infection at the population level. The results show that pigs can be placed into distinct phenotypic groups, including pigs that show resistance or pigs that exhibit tolerance to infection. Tolerance is best illustrated by pigs that gain weight normally in the face of a relatively high virus load. Genome-wide association analysis has identified a region on chromosome 4 (SSC4 correlated with resistance; i.e., higher weight gain combined with lower virus load. The genomic region is near a family of genes involved in innate immunity. These results create the opportunity to develop breeding programs that will produce pigs with increased resistance to PRRS. The identification of genomic markers involved in tolerance will likely prove more difficult, primarily because tolerance

  19. Stability and Time Delay Tolerance Analysis Approach for Networked Control Systems

    Directory of Open Access Journals (Sweden)

    Ashraf F. Khalil

    2015-01-01

    Full Text Available Networked control system is a research area where the theory is behind practice. Closing the feedback loop through shared network induces time delay and some of the data could be lost. So the network induced time delay and data loss are inevitable in networked control Systems. The time delay may degrade the performance of control systems or even worse lead to system instability. Once the structure of a networked control system is confirmed, it is essential to identify the maximum time delay allowed for maintaining the system stability which, in turn, is also associated with the process of controller design. Some studies reported methods for estimating the maximum time delay allowed for maintaining system stability; however, most of the reported methods are normally overcomplicated for practical applications. A method based on the finite difference approximation is proposed in this paper for estimating the maximum time delay tolerance, which has a simple structure and is easy to apply.

  20. A hybrid robust fault tolerant control based on adaptive joint unscented Kalman filter.

    Science.gov (United States)

    Shabbouei Hagh, Yashar; Mohammadi Asl, Reza; Cocquempot, Vincent

    2017-01-01

    In this paper, a new hybrid robust fault tolerant control scheme is proposed. A robust H∞ control law is used in non-faulty situation, while a Non-Singular Terminal Sliding Mode (NTSM) controller is activated as soon as an actuator fault is detected. Since a linear robust controller is designed, the system is first linearized through the feedback linearization method. To switch from one controller to the other, a fuzzy based switching system is used. An Adaptive Joint Unscented Kalman Filter (AJUKF) is used for fault detection and diagnosis. The proposed method is based on the simultaneous estimation of the system states and parameters. In order to show the efficiency of the proposed scheme, a simulated 3-DOF robotic manipulator is used.

  1. Fault-Tolerant Region-Based Control of an Underwater Vehicle with Kinematically Redundant Thrusters

    Directory of Open Access Journals (Sweden)

    Zool H. Ismail

    2014-01-01

    Full Text Available This paper presents a new control approach for an underwater vehicle with a kinematically redundant thruster system. This control scheme is derived based on a fault-tolerant decomposition for thruster force allocation and a region control scheme for the tracking objective. Given a redundant thruster system, that is, six or more pairs of thrusters are used, the proposed redundancy resolution and region control scheme determine the number of thruster faults, as well as providing the reference thruster forces in order to keep the underwater vehicle within the desired region. The stability of the presented control law is proven in the sense of a Lyapunov function. Numerical simulations are performed with an omnidirectional underwater vehicle and the results of the proposed scheme illustrate the effectiveness in terms of optimizing the thruster forces.

  2. Deciphering the genetic regulatory code using an inverse error control coding framework.

    Energy Technology Data Exchange (ETDEWEB)

    Rintoul, Mark Daniel; May, Elebeoba Eni; Brown, William Michael; Johnston, Anna Marie; Watson, Jean-Paul

    2005-03-01

    We have found that developing a computational framework for reconstructing error control codes for engineered data and ultimately for deciphering genetic regulatory coding sequences is a challenging and uncharted area that will require advances in computational technology for exact solutions. Although exact solutions are desired, computational approaches that yield plausible solutions would be considered sufficient as a proof of concept to the feasibility of reverse engineering error control codes and the possibility of developing a quantitative model for understanding and engineering genetic regulation. Such evidence would help move the idea of reconstructing error control codes for engineered and biological systems from the high risk high payoff realm into the highly probable high payoff domain. Additionally this work will impact biological sensor development and the ability to model and ultimately develop defense mechanisms against bioagents that can be engineered to cause catastrophic damage. Understanding how biological organisms are able to communicate their genetic message efficiently in the presence of noise can improve our current communication protocols, a continuing research interest. Towards this end, project goals include: (1) Develop parameter estimation methods for n for block codes and for n, k, and m for convolutional codes. Use methods to determine error control (EC) code parameters for gene regulatory sequence. (2) Develop an evolutionary computing computational framework for near-optimal solutions to the algebraic code reconstruction problem. Method will be tested on engineered and biological sequences.

  3. Dynamic control of the complement system by modulated expression of regulatory proteins.

    Science.gov (United States)

    Thurman, Joshua M; Renner, Brandon

    2011-01-01

    The complement system serves many biological functions, including the eradication of invasive pathogens and the removal of damaged cells and immune-complexes. Uncontrolled complement activation causes injury to host cells, however, so adequate regulation of the system is essential. Control of the complement system is maintained by a group of cell surface and circulating proteins referred to as complement regulatory proteins. The expression of the cell surface complement regulatory proteins varies from tissue to tissue. Furthermore, specific cell types can upregulate or downregulate the expression of these proteins in response to a variety of signals or insults. Altered regulation of the complement regulatory proteins can have important effects on local complement activation. In some circumstances this can be beneficial, such as in the setting of certain infections. In other circumstances, however, this can be a cause of complement-mediated injury of the tissue. A full understanding of the mechanisms by which the complement system is modulated at the local level can have important implications for how we diagnose and treat a wide range of inflammatory diseases.

  4. Fault Tolerant Architecture For A Fly-By-Light Flight Control Computer

    Science.gov (United States)

    Thompson, Kevin; Stipanovich, John; Smith, Brian; Reddy, Mahesh C.

    1990-02-01

    The next generation of flight control computers will utilize fiber optic technology to produce a fly-by-light flight control system. Optical transducers and optical fibers will take the place of electrical position transducers and wires, torsion bars, bell cranks, and cables. Applications for this fly-by-light technology include space launch vehicles, upperstages, space-craft, and commercial/military aircraft. Optical fibers are lighter than mechanical transmission media and unlike conven-tional wire transmissions are not susceptible to electromagnetic interference (EMI) and high energy emission sources. This paper will give an overview of a fault tolerant In-Line Monitored optical flight control system being developed at Boeing Aerospace & Electronics in Seattle, Washington. This system uses passive transducers with fiber optic interconnections which hold promises to virtually eliminate EMI threats to flight control system performance and flight safety and also provide significant weight savings. The main emphasis of this paper will be the In-Line Monitored architecture of the optical transducer system required for use in a fault tolerant flight control system.

  5. Organization, integration, and assembly of genetic and epigenetic regulatory machinery in nuclear microenvironments: implications for biological control in cancer.

    Science.gov (United States)

    Stein, Gary S; Zaidi, Sayyed K; Stein, Janet L; Lian, Jane B; van Wijnen, Andre J; Montecino, Martin; Young, Daniel W; Javed, Amjad; Pratap, Jitesh; Choi, Je-Yong; Ali, Syed A; Pande, Sandhya; Hassan, Mohammad Q

    2009-02-01

    There is growing awareness that the fidelity of gene expression necessitates coordination of transcription factor metabolism and organization of genes and regulatory proteins within the three-dimensional context of nuclear architecture. The regulatory machinery that governs genetic and epigenetic control of gene expression is compartmentalized in nuclear microenvironments. Temporal and spatial parameters of regulatory complex organization and assembly are functionally linked to biological control and are compromised with the onset and progression of tumorigenesis. High throughput imaging of cells, tissues, and tumors, including live cell analysis, is expanding research's capabilities toward translating components of nuclear organization into novel strategies for cancer diagnosis and therapy.

  6. Exploring the intricate regulatory network controlling the thiazide-sensitive NaCl cotransporter (NCC)

    DEFF Research Database (Denmark)

    Dimke, Henrik Anthony

    2011-01-01

    The thiazide-sensitive NaCl cotransporter (NCC) plays key roles in renal electrolyte transport and blood pressure maintenance. Regulation of this cotransporter has received increased attention recently, prompted by the discovery that mutations in the with-no-lysine (WNK) kinases are the molecular...... by acting as a scaffold between the dephosphorylated cotransporter and the regulatory kinase. As more molecular regulators of NCC are identified, the system-controlling NCC activity is becoming increasingly complex. This intricacy confers an ability to integrate a variety of stimuli, thereby regulating NCC...

  7. A reconfigurable damage-tolerant controller based on a modal double-loop framework

    Science.gov (United States)

    Genari, Helói F. G.; Mechbal, Nazih; Coffignal, Gérard; Nóbrega, Eurípedes G. O.

    2017-05-01

    Active vibration control of flexible structures has received considerable attention in the latest decades. However, several related control problems remain open to new investigations such as robust performance, spillover instability, and structural changes due to damage. Specifically in the case of damage, it may significantly aggravate closed-loop performance. Damage-tolerant active control is a recent research area that includes structural damage effect reduction in the controller design requirements. This paper presents a novel control method based on a modal double-loop controller design, aiming for vibration reduction of noncollocated flexible structures subject to damage and encompassing online reconfigurability. The first controller is designed for the healthy system in order to comply with predefined performance and robustness requirements, based on modal H∞ norm. The second controller complements the closed-loop performance if the structure is damaged. A reconfigurable modal technique is adopted to design the second controller, using online modal structural parameter change information to update the controller. To assess the proposed method, finite element models are developed for a case study structure, including health and damage conditions. Results show the effectiveness of the methodology along with performance improvement compared to single-loop controllers based on regular H∞ and modal H∞ approaches.

  8. Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants

    OpenAIRE

    Todaka, Daisuke; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko

    2015-01-01

    Advances have been made in the development of drought-tolerant transgenic plants, including cereals. Rice, one of the most important cereals, is considered to be a critical target for improving drought tolerance, as present-day rice cultivation requires large quantities of water and as drought-tolerant rice plants should be able to grow in small amounts of water. Numerous transgenic rice plants showing enhanced drought tolerance have been developed to date. Such genetically engineered plants ...

  9. A Fault-tolerable Control Scheme for an Open-frame Underwater Vehicle

    Directory of Open Access Journals (Sweden)

    Huang Hai

    2014-05-01

    Full Text Available Open-frame is one of the major types of structures of Remote Operated Vehicles (ROV because it is easy to place sensors and operations equipment on- board. Firstly, this paper designed a petri-based recurrent neural network (PRFNN to improve the robustness with response to nonlinear characteristics and strong disturbance of an open-frame underwater vehicle. A threshold has been set in the third layer to reduce the amount of calculations and regulate the training process. The whole network convergence is guaranteed with the selection of learning rate parameters. Secondly, a fault tolerance control (FTC scheme is established with the optimal allocation of thrust. Infinity-norm optimization has been combined with 2-norm optimization to construct a bi-criteria primal-dual neural network FTC scheme. In the experiments and simulation, PRFNN outperformed fuzzy neural networks in motion control, while bi-criteria optimization outperformed 2-norm optimization in FTC, which demonstrates that the FTC controller can improve computational efficiency, reduce control errors, and implement fault tolerable thrust allocation.

  10. Controlling the Self-organizing Dynamics in a Sandpile Model on Complex Networks by Failure Tolerance

    Energy Technology Data Exchange (ETDEWEB)

    Qi, J.; Pfenninger, S.

    2015-08-01

    In this paper, we propose a strategy to control the self-organizing dynamics of the Bak-Tang-Wiesenfeld (BTW) sandpile model on complex networks by allowing some degree of failure tolerance for the nodes and introducing additional active dissipation while taking the risk of possible node damage. We show that the probability for large cascades significantly increases or decreases respectively when the risk for node damage outweighs the active dissipation and when the active dissipation outweighs the risk for node damage. By considering the potential additional risk from node damage, a non-trivial optimal active dissipation control strategy which minimizes the total cost in the system can be obtained. Under some conditions the introduced control strategy can decrease the total cost in the system compared to the uncontrolled model. Moreover, when the probability of damaging a node experiencing failure tolerance is greater than the critical value, then no matter how successful the active dissipation control is, the total cost of the system will have to increase. This critical damage probability can be used as an indicator of the robustness of a network or system. Copyright (C) EPLA, 2015

  11. Nrf2 as a master regulator of tissue damage control and disease tolerance to infection.

    Science.gov (United States)

    Soares, Miguel P; Ribeiro, Ana M

    2015-08-01

    Damage control refers to those actions made towards minimizing damage or loss. Depending on the context, these can range from emergency procedures dealing with the sinking of a ship or to a surgery dealing with severe trauma or even to an imaginary company in Marvel comics, which repairs damaged property arising from conflicts between super heroes and villains. In the context of host microbe interactions, tissue damage control refers to an adaptive response that limits the extent of tissue damage associated with infection. Tissue damage control can limit the severity of infectious diseases without interfering with pathogen burden, conferring disease tolerance to infection. This contrasts with immune-driven resistance mechanisms, which although essential to protect the host from infection, can impose tissue damage to host parenchyma tissues. This damaging effect is countered by stress responses that confer tissue damage control and disease tolerance to infection. Here we discuss how the stress response regulated by the transcription factor nuclear factor-erythroid 2-related factor 2 (Nrf2) acts in such a manner. © 2015 Authors.

  12. Nrf2 as a master regulator of tissue damage control and disease tolerance to infection

    Science.gov (United States)

    Soares, Miguel P.; Ribeiro, Ana M.

    2015-01-01

    Damage control refers to those actions made towards minimizing damage or loss. Depending on the context, these can range from emergency procedures dealing with the sinking of a ship or to a surgery dealing with severe trauma or even to an imaginary company in Marvel comics, which repairs damaged property arising from conflicts between super heroes and villains. In the context of host microbe interactions, tissue damage control refers to an adaptive response that limits the extent of tissue damage associated with infection. Tissue damage control can limit the severity of infectious diseases without interfering with pathogen burden, conferring disease tolerance to infection. This contrasts with immune-driven resistance mechanisms, which although essential to protect the host from infection, can impose tissue damage to host parenchyma tissues. This damaging effect is countered by stress responses that confer tissue damage control and disease tolerance to infection. Here we discuss how the stress response regulated by the transcription factor nuclear factor-erythroid 2-related factor 2 (Nrf2) acts in such a manner. PMID:26551709

  13. A Fault-tolerable Control Scheme for an Open-frame Underwater Vehicle

    Directory of Open Access Journals (Sweden)

    Huang Hai

    2014-05-01

    Full Text Available Open-frame is one of the major types of structures of Remote Operated Vehicles (ROV because it is easy to place sensors and operations equipment onboard. Firstly, this paper designed a petri-based recurrent neural network (PRFNN to improve the robustness with response to nonlinear characteristics and strong disturbance of an open-frame underwater vehicle. A threshold has been set in the third layer to reduce the amount of calculations and regulate the training process. The whole network convergence is guaranteed with the selection of learning rate parameters. Secondly, a fault tolerance control (FTC scheme is established with the optimal allocation of thrust. Infinity-norm optimization has been combined with 2-norm optimization to construct a bi-criteria primal-dual neural network FTC scheme. In the experiments and simulation, PRFNN outperformed fuzzy neural networks in motion control, while bi-criteria optimization outperformed 2-norm optimization in FTC, which demonstrates that the FTC controller can improve computational efficiency, reduce control errors, and implement fault tolerable thrust allocation.

  14. Fault-Tolerant Scheduling for Real-Time Embedded Control Systems

    Institute of Scientific and Technical Information of China (English)

    Chun-Hua Yang; Geert Deconinck; Wei-Hua Gui

    2004-01-01

    With the increasing complexity of industrial application, an embedded control system (ECS) requires processing a number of hard real-time tasks and needs fault-tolerance to assure high reliability. Considering the characteristics of real-time tasks in ECS, an integrated algorithm is proposed to schedule real-time tasks and to guarantee that all real-time tasks are completed before their deadlines even in the presence of faults. Based on the nonpreemptive critical-section protocol (NCSP), this paper analyzes the blocking time introduced by resource conflicts of relevancy tasks in fault-tolerant multiprocessor systems. An extended schedulability condition is presented to check the assignment feasibility of a given task to a processor. A primary/backup approach and on-line replacement of failed processors are used to tolerate processor failures. The analysis reveals that the integrated algorithm bounds the blocking time, requires limited overhead on the number of processors, and still assures good processor utilization. This is also demonstrated by simulation results. Both analysis and simulation show the effectiveness of the proposed algorithm in ECS.

  15. Robust Fault Tolerant Control for a Class of Time-Delay Systems with Multiple Disturbances

    Directory of Open Access Journals (Sweden)

    Songyin Cao

    2013-01-01

    Full Text Available A robust fault tolerant control (FTC approach is addressed for a class of nonlinear systems with time delay, actuator faults, and multiple disturbances. The first part of the multiple disturbances is supposed to be an uncertain modeled disturbance and the second one represents a norm-bounded variable. First, a composite observer is designed to estimate the uncertain modeled disturbance and actuator fault simultaneously. Then, an FTC strategy consisting of disturbance observer based control (DOBC, fault accommodation, and a mixed H2/H∞ controller is constructed to reconfigure the considered systems with disturbance rejection and attenuation performance. Finally, simulations for a flight control system are given to show the efficiency of the proposed approach.

  16. A Fault-Tolerant Emergency-Aware Access Control Scheme for Cyber-Physical Systems

    CERN Document Server

    Wu, Guowei; Xia, Feng; Yao, Lin

    2012-01-01

    Access control is an issue of paramount importance in cyber-physical systems (CPS). In this paper, an access control scheme, namely FEAC, is presented for CPS. FEAC can not only provide the ability to control access to data in normal situations, but also adaptively assign emergency-role and permissions to specific subjects and inform subjects without explicit access requests to handle emergency situations in a proactive manner. In FEAC, emergency-group and emergency-dependency are introduced. Emergencies are processed in sequence within the group and in parallel among groups. A priority and dependency model called PD-AGM is used to select optimal response-action execution path aiming to eliminate all emergencies that occurred within the system. Fault-tolerant access control polices are used to address failure in emergency management. A case study of the hospital medical care application shows the effectiveness of FEAC.

  17. Development of an interface for an ultrareliable fault-tolerant control system and an electronic servo-control unit

    Science.gov (United States)

    Shaver, Charles; Williamson, Michael

    1986-01-01

    The NASA Ames Research Center sponsors a research program for the investigation of Intelligent Flight Control Actuation systems. The use of artificial intelligence techniques in conjunction with algorithmic techniques for autonomous, decentralized fault management of flight-control actuation systems is explored under this program. The design, development, and operation of the interface for laboratory investigation of this program is documented. The interface, architecturally based on the Intel 8751 microcontroller, is an interrupt-driven system designed to receive a digital message from an ultrareliable fault-tolerant control system (UFTCS). The interface links the UFTCS to an electronic servo-control unit, which controls a set of hydraulic actuators. It was necessary to build a UFTCS emulator (also based on the Intel 8751) to provide signal sources for testing the equipment.

  18. Evaluation of availability of cluster distributed disaster tolerant systems for control and information processing based on a cluster quorum

    Science.gov (United States)

    Tsarev, R. Yu; Gruzenkin, D. V.; Kovalev, I. V.; Prokopenko, A. V.; Knyazkov, A. N.

    2016-11-01

    Control and information processing systems, which often executes critical functions, must satisfy requirements not only of fault tolerance, but also of disaster tolerance. Cluster architecture is reasonable to be applied to provide disaster tolerance of these systems. In this case clusters are separate control and information processing centers united by means of communication channels. Thus, clusters are a single hardware resource interacting with each other to achieve system objectives. Remote cluster positioning allows ensuring system availability and disaster tolerance even in case of some units’ failures or a whole cluster crash. A technique for evaluation of availability of cluster distributed systems for control and information processing based on a cluster quorum is presented in the paper. This technique can be applied to different cluster distributed control and information processing systems, claimed to be based on the disaster tolerance principles. In the article we discuss a communications satellite system as an example of a cluster distributed disaster tolerant control and information processing system. Evaluation of availability of the communications satellite system is provided. Possible scenarios of communications satellite system cluster-based components failures were analyzed. The analysis made it possible to choose the best way to implement the cluster structure for a distributed control and information processing system.

  19. Fault Tolerant Attitude Control for spacecraft with SGCMGs under actuator partial failure and actuator saturation

    Science.gov (United States)

    Zhang, Fuzhen; Jin, Lei; Xu, Shijie

    2017-03-01

    A Fault Tolerant Attitude Control algorithm for the spacecraft using Single Gimbal Control Moment Gyros (SGCMGs) as actuator is proposed. The controller is designed using the sliding mode control theory to control the gimbal rate directly and there is no singular point in the control algorithm, which means that we don't need to design the steering laws again and the singularity problems can be avoided. Also the gimbal rate saturation is considered when designing the control method. The adaptive control algorithm is used to estimate the disturbance and the boundary of the fault and saturation, which means that no prior information of the fault is needed. Although the controller is designed based on the SGCMGs, it can also be employed when reaction wheels work as the actuator of the spacecraft. Also the complete failure of several SGCMGs is allowed. It is proved based on the Lyapunov stability theorem that the designed control algorithm can achieve the attitude asymptotic stability both on the fault or fault-free condition. The simulation results show that the proposed method has a strong robustness.

  20. Fault-tolerant control of electric vehicles with in-wheel motors using actuator-grouping sliding mode controllers

    Science.gov (United States)

    Li, Boyuan; Du, Haiping; Li, Weihua

    2016-05-01

    Although electric vehicles with in-wheel motors have been regarded as one of the promising vehicle architectures in recent years, the probability of in-wheel motor fault is still a crucial issue due to the system complexity and large number of control actuators. In this study, a modified sliding mode control (SMC) is applied to achieve fault-tolerant control of electric vehicles with four-wheel-independent-steering (4WIS) and four-wheel-independent-driving (4WID). Unlike in traditional SMC, in this approach the steering geometry is re-arranged according to the location of faulty wheels in the modified SMC. Three SMC control laws for longitudinal velocity control, lateral velocity control and yaw rate control are designed based on specific vehicle motion scenarios. In addition the actuator-grouping SMC method is proposed so that driving actuators are grouped and each group of actuators can be used to achieve the specific control target, which avoids the strong coupling effect between each control target. Simulation results prove that the proposed modified SMC can achieve good vehicle dynamics control performance in normal driving and large steering angle turning scenarios. In addition, the proposed actuator-grouping SMC can solve the coupling effect of different control targets and the control performance is improved.

  1. Regulatory elements and transcriptional control of chicken vasa homologue (CVH) promoter in chicken primordial germ cells.

    Science.gov (United States)

    Jin, So Dam; Lee, Bo Ram; Hwang, Young Sun; Lee, Hong Jo; Rim, Jong Seop; Han, Jae Yong

    2017-01-01

    Primordial germ cells (PGCs), the precursors of functional gametes, have distinct characteristics and exhibit several unique molecular mechanisms to maintain pluripotency and germness in comparison to somatic cells. They express germ cell-specific RNA binding proteins (RBPs) by modulating tissue-specific cis- and trans-regulatory elements. Studies on gene structures of chicken vasa homologue (CVH), a chicken RNA binding protein, involved in temporal and spatial regulation are thus important not only for understanding the molecular mechanisms that regulate germ cell fate, but also for practical applications of primordial germ cells. However, very limited studies are available on regulatory elements that control germ cell-specific expression in chicken. Therefore, we investigated the intricate regulatory mechanism(s) that governs transcriptional control of CVH. We constructed green fluorescence protein (GFP) or luciferase reporter vectors containing the various 5' flanking regions of CVH gene. From the 5' deletion and fragmented assays in chicken PGCs, we have identified a CVH promoter that locates at -316 to +275 base pair fragment with the highest luciferase activity. Additionally, we confirmed for the first time that the 5' untranslated region (UTR) containing intron 1 is required for promoter activity of the CVH gene in chicken PGCs. Furthermore, using a transcription factor binding prediction, transcriptome analysis and siRNA-mediated knockdown, we have identified that a set of transcription factors play a role in the PGC-specific CVH gene expression. These results demonstrate that cis-elements and transcription factors localizing in the 5' flanking region including the 5' UTR and an intron are important for transcriptional regulation of the CVH gene in chicken PGCs. Finally, this information will contribute to research studies in areas of reproductive biology, constructing of germ cell-specific synthetic promoter for tracing primordial germ cells as well as

  2. Sub-circuits of a gene regulatory network control a developmental epithelial-mesenchymal transition.

    Science.gov (United States)

    Saunders, Lindsay R; McClay, David R

    2014-04-01

    Epithelial-mesenchymal transition (EMT) is a fundamental cell state change that transforms epithelial to mesenchymal cells during embryonic development, adult tissue repair and cancer metastasis. EMT includes a complex series of intermediate cell state changes including remodeling of the basement membrane, apical constriction, epithelial de-adhesion, directed motility, loss of apical-basal polarity, and acquisition of mesenchymal adhesion and polarity. Transcriptional regulatory state changes must ultimately coordinate the timing and execution of these cell biological processes. A well-characterized gene regulatory network (GRN) in the sea urchin embryo was used to identify the transcription factors that control five distinct cell changes during EMT. Single transcription factors were perturbed and the consequences followed with in vivo time-lapse imaging or immunostaining assays. The data show that five different sub-circuits of the GRN control five distinct cell biological activities, each part of the complex EMT process. Thirteen transcription factors (TFs) expressed specifically in pre-EMT cells were required for EMT. Three TFs highest in the GRN specified and activated EMT (alx1, ets1, tbr) and the 10 TFs downstream of those (tel, erg, hex, tgif, snail, twist, foxn2/3, dri, foxb, foxo) were also required for EMT. No single TF functioned in all five sub-circuits, indicating that there is no EMT master regulator. Instead, the resulting sub-circuit topologies suggest EMT requires multiple simultaneous regulatory mechanisms: forward cascades, parallel inputs and positive-feedback lock downs. The interconnected and overlapping nature of the sub-circuits provides one explanation for the seamless orchestration by the embryo of cell state changes leading to successful EMT.

  3. Industrial Cost-Benefit Assessment for Fault-tolerant Control Systems

    DEFF Research Database (Denmark)

    Thybo, C.; Blanke, M.

    1998-01-01

    against failure. The paper describes the assessments needed to find the right path for new industrial designs. The economic decisions in the design phase are discussed: cost of different failures, profits associated with available benefits, investments needed for development and life-time support....... The objective of this paper is to help, in the early product development state, to find the economical most suitable scheme. A salient result is that with increased customer awareness of total cost of ownership, new products can benefit significantly from applying fault tolerant control principles....

  4. Fault Detection and Isolation and Fault Tolerant Control of Wind Turbines Using Set-Valued Observers

    DEFF Research Database (Denmark)

    Casau, Pedro; Rosa, Paulo Andre Nobre; Tabatabaeipour, Seyed Mojtaba

    2012-01-01

    and Isolation (FDI) and Fault Tolerant Control (FTC) of wind turbines, by taking advantage of the recent advances in SVO theory for model invalidation. A simple wind turbine model is presented along with possible faulty scenarios. The FDI algorithm is built on top of the described model, taking into account......Research on wind turbine Operations & Maintenance (O&M) procedures is critical to the expansion of Wind Energy Conversion systems (WEC). In order to reduce O&M costs and increase the lifespan of the turbine, we study the application of Set-Valued Observers (SVO) to the problem of Fault Detection...

  5. Fault Diagnosis and Fault Tolerant Control with Application on a Wind Turbine Low Speed Shaft Encoder

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Sardi, Hector Eloy Sanchez; Escobet, Teressa

    2015-01-01

    . This sensor has to be correct as blade pitch actions should be different at different azimuth angle as the wind speed varies within the rotor field due to different phenomena. A scheme detecting faults in this sensor has previously been designed for the application of a high end fault diagnosis and fault...... tolerant control of wind turbines using a benchmark model. In this paper, the fault diagnosis scheme is improved and integrated with a fault accommodation scheme which enables and disables the individual pitch algorithm based on the fault detection. In this way, the blade and tower loads are not increased...

  6. Fault Diagnosis and Fault-Tolerant Control of Wind Turbines via a Discrete Time Controller with a Disturbance Compensator

    Directory of Open Access Journals (Sweden)

    Yolanda Vidal

    2015-05-01

    Full Text Available This paper develops a fault diagnosis (FD and fault-tolerant control (FTC of pitch actuators in wind turbines. This is accomplished by combining a disturbance compensator with a controller, both of which are formulated in the discrete time domain. The disturbance compensator has a dual purpose: to estimate the actuator fault (which is used by the FD algorithm and to design the discrete time controller to obtain an FTC. That is, the pitch actuator faults are estimated, and then, the pitch control laws are appropriately modified to achieve an FTC with a comparable behavior to the fault-free case. The performance of the FD and FTC schemes is tested in simulations with the aero-elastic code FAST.

  7. Fault diagnosis and fault-tolerant control and guidance for aerospace vehicles from theory to application

    CERN Document Server

    Zolghadri, Ali; Cieslak, Jerome; Efimov, Denis; Goupil, Philippe

    2014-01-01

    Fault Diagnosis and Fault-Tolerant Control and Guidance for Aerospace demonstrates the attractive potential of recent developments in control for resolving such issues as improved flight performance, self-protection and extended life of structures. Importantly, the text deals with a number of practically significant considerations: tuning, complexity of design, real-time capability, evaluation of worst-case performance, robustness in harsh environments, and extensibility when development or adaptation is required. Coverage of such issues helps to draw the advanced concepts arising from academic research back towards the technological concerns of industry. Initial coverage of basic definitions and ideas and a literature review gives way to a treatment of important electrical flight control system failures: the oscillatory failure case, runaway, and jamming. Advanced fault detection and diagnosis for linear and nonlinear systems are described. Lastly recovery strategies appropriate to remaining acuator/sensor/c...

  8. Distributed adaptive fault-tolerant control against actuator faults and lossy interconnection links

    Institute of Scientific and Technical Information of China (English)

    Xiaozheng JIN; Guanghong YANG

    2009-01-01

    This paper presents an adaptive method to solve the robust fault-tolerant control (FTC) problem for a class of large scale systems against actuator failures and lossy interconnection links. In terms of the special distributed architectures, the adaptation laws are proposed to estimate the unknown eventual faults of actuators and interconnections, constant external disturbances, and controller parameters on-line. Then a class of distributed state feedback controllers are constructed for automatically compensating the fault and disturbance effects on systems based on the information from adaptive schemes. On the basis of Lyapunov stability theory, it shows that the resulting adaptive closed-loop large-scale system can be guaranteed to be asymptotically stable in the presence of uncertain faults of actuators and interconnections, and constant disturbances. The proposed design technique is finally evaluated in the light of a simulation example.

  9. Speed/position sensor fault tolerant control in adjustable speed drives - A review.

    Science.gov (United States)

    Bourogaoui, M; Sethom, H Ben Attia; Belkhodja, I Slama

    2016-09-01

    The position sensor is one of the most used devices in Adjustable Speed Drives (ASDs). Its use is mandatory in electric machines vector control. In this paper, an interest was addressed to this field. Indeed, a bibliographical review, about Fault Detection and Isolation (FDI) and Fault Tolerant Control (FTC) in ASDs, is presented. Thus, the paper deals with position sensor FDI and sensorless control-based FTC in ASDs. Moreover, this issue is mainly addressed to position sensor faults in ASDs. This paper is based on a wide literature review referring to scientific papers and manufacturer׳s technical documents. In total, 186 references in the open literature, dating back to 1981, have been investigated in order to perform this study.

  10. Decentralized Fault-Tolerant Control of Inland Navigation Networks: a Challenge

    Science.gov (United States)

    Segovia, P.; Rajaoarisoa, L.; Nejjari, F.; Blesa, J.; Puig, V.; Duviella, E.

    2017-01-01

    Inland waterways are large-scale networks used principally for navigation. Even if the transport planning is an important issue, the water resource management is a crucial point. Indeed, navigation is not possible when there is too little or too much water inside the waterways. Hence, the water resource management of waterways has to be particularly efficient in a context of climate change and increase of water demand. This management has to be done by considering different time and space scales and still requires the development of new methodologies and tools in the topics of the Control and Informatics communities. This work addresses the problem of waterways management in terms of modeling, control, diagnosis and fault-tolerant control by focusing in the inland waterways of the north of France. A review of proposed tools and the ongoing research topics are provided in this paper.

  11. Circulating T helper and T regulatory subsets in untreated early rheumatoid arthritis and healthy control subjects.

    Science.gov (United States)

    Pandya, Jayesh M; Lundell, Anna-Carin; Hallström, Magnus; Andersson, Kerstin; Nordström, Inger; Rudin, Anna

    2016-10-01

    The pathogenic role and frequency of T cell subtypes in early rheumatoid arthritis are still unclear. We therefore performed a comprehensive analysis of the circulating T cell subtype pattern in patients with untreated early rheumatoid arthritis compared to healthy control subjects. Peripheral blood mononuclear cells were obtained from 26 patients with untreated early rheumatoid arthritis and from with 18 age- and sex-matched healthy control subjects. T helper cell types Th0, Th1, Th2, Th17, and Th1/17 and nonclassic T helper subsets were defined by flow cytometry based on the expression of chemokine receptors CCR4, CCR6, and CXCR3. Regulatory T cells were defined by expression of CD25(+) CD127(low) and also FOXP3 CXCR5(+) cells among regulatory and nonregulatory T cells were defined as T follicular regulatory and T follicular helper cells, respectively. The phenotype of T cell subsets was confirmed by transcription factor and cytokine secretion analyses. Multivariate discriminant analysis showed that patients with untreated early rheumatoid arthritis were segregated from healthy control subjects based on the circulating T cell subset profile. Among the discriminator subsets, CCR4(+)CXCR3(-) (Th2 and Th17), CTLA4(+) and FOXP3(+) subsets were present in significantly higher frequencies, whereas CCR4(-) (Th1/Th17, CCR6(+)CCR4(-)CXCR3(-), and Th1) subsets were present in lower frequencies in patients with untreated early rheumatoid arthritis compared with healthy control subjects. The proportions of Th2 and Th17 subsets associated positively with each other and negatively with the CXCR3(+)/interferon γ-secreting subsets (Th1 and Th1/Th17) in patients with untreated rheumatoid arthritis. The proportions of Th2 cells increased with age in patients with untreated early rheumatoid arthritis and healthy control subjects. The dominance of circulating CCR4(+)CXCR3(-) T helper subsets (Th2 and Th17) in untreated early rheumatoid arthritis point toward a pathogenic role of

  12. TNF phase III signalling in tolerant cells is tightly controlled by A20 and CYLD.

    Science.gov (United States)

    Bikker, Rolf; Christmann, Martin; Preuß, Katharina; Welz, Bastian; Friesenhagen, Judith; Dittrich-Breiholz, Oliver; Huber, René; Brand, Korbinian

    2017-09-01

    Following the acute phase of an inflammatory reaction, a strictly controlled resolution of inflammation is necessary. A dysregulation of this process leads to hyperinflammation, chronic inflammatory disease, or immune paralysis. Different mechanisms participate in the coordinated termination of the inflammatory process, e.g. the expression of antiinflammatory molecules and different forms of tolerance. To better understand the processes which mediate resolution of TNF-dependent inflammation and induce tolerance, it is necessary to characterize the signal transduction quality during TNF long-term (pre)incubation. Within a time frame from 12 to 48h, designated as phase III of the TNF response, we measured an ongoing, constitutive activation of TNFR1/NF-κB-dependent pathways in monocytic cells. Phase III signalling which was also named "constitutive signaling in TNF tolerant cells" induces the expression of low- and high-sensitive target genes including A20 which is differentially regulated by transcriptional and proteolytic events. A20 strictly controls TNF long-term constitutive signalling in an IκB kinase complex- and partially RIP-dependent manner supported by adjuvant ABIN1. In addition, CYLD proteins participate in the regulation of this late-phase signal transduction, whereas downstream molecules such as Bcl3 and p50 are not involved. A20 and CYLD are expressed with different mRNA kinetics resulting in a strong or only a modest increase in protein levels, respectively. The identification of mechanisms which contribute to the termination of inflammation will provide additional diagnostic and therapeutic aspects to specifically diagnose certain aspects of inflammation and specifically modulate them. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Specific interactions between DNA and regulatory protein controlled by ligand-binding: Ab initio molecular simulation

    Energy Technology Data Exchange (ETDEWEB)

    Matsushita, Y., E-mail: kurita@cs.tut.ac.jp; Murakawa, T., E-mail: kurita@cs.tut.ac.jp; Shimamura, K., E-mail: kurita@cs.tut.ac.jp; Oishi, M., E-mail: kurita@cs.tut.ac.jp; Ohyama, T., E-mail: kurita@cs.tut.ac.jp; Kurita, N., E-mail: kurita@cs.tut.ac.jp [Department of Computer Science and Engineering, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, 441-8580 (Japan)

    2015-02-27

    The catabolite activator protein (CAP) is one of the regulatory proteins controlling the transcription mechanism of gene. Biochemical experiments elucidated that the complex of CAP with cyclic AMP (cAMP) is indispensable for controlling the mechanism, while previous molecular simulations for the monomer of CAP+cAMP complex revealed the specific interactions between CAP and cAMP. However, the effect of cAMP-binding to CAP on the specific interactions between CAP and DNA is not elucidated at atomic and electronic levels. We here considered the ternary complex of CAP, cAMP and DNA in solvating water molecules and investigated the specific interactions between them at atomic and electronic levels using ab initio molecular simulations based on classical molecular dynamics and ab initio fragment molecular orbital methods. The results highlight the important amino acid residues of CAP for the interactions between CAP and cAMP and between CAP and DNA.

  14. Fault tolerant control based on interval type-2 fuzzy sliding mode controller for coaxial trirotor aircraft.

    Science.gov (United States)

    Zeghlache, Samir; Kara, Kamel; Saigaa, Djamel

    2015-11-01

    In this paper, a robust controller for a Six Degrees of Freedom (6 DOF) coaxial trirotor helicopter control is proposed in presence of defects in the system. A control strategy based on the coupling of the interval type-2 fuzzy logic control and sliding mode control technique are used to design a controller. The main purpose of this work is to eliminate the chattering phenomenon and guaranteeing the stability and the robustness of the system. In order to achieve this goal, interval type-2 fuzzy logic control has been used to generate the discontinuous control signal. The simulation results have shown that the proposed control strategy can greatly alleviate the chattering effect, and perform good reference tracking in presence of defects in the system.

  15. Eating on impulse: Implicit attitudes, self-regulatory resources, and trait self-control as determinants of food consumption.

    Science.gov (United States)

    Wang, Yan; Wang, Lei; Cui, Xianghua; Fang, Yuan; Chen, Qianqiu; Wang, Ya; Qiang, Yao

    2015-12-01

    Self-regulatory resources and trait self-control have been found to moderate the impulse-behavior relationship. The current study investigated whether the interaction of self-regulatory resources and trait self-control moderates the association between implicit attitudes and food consumption. One hundred twenty female participants were randomly assigned to either a depletion condition in which their self-regulatory resources were reduced or a no-depletion condition. Participants' implicit attitudes for chocolate were measured with the Single Category Implicit Association Test and self-report measures of trait self-control were collected. The dependent variable was chocolate consumption in an ostensible taste and rate task. Implicit attitudes predicted chocolate consumption in depleted participants but not in non-depleted participants. However, this predictive power of implicit attitudes on eating in depleted condition disappeared in participants with high trait self-control. Thus, trait self-control and self-regulatory resources interact to moderate the prediction of implicit attitude on eating behavior. Results suggest that high trait self-control buffers the effect of self-regulatory depletion on impulsive eating.

  16. Robust fault tolerant control based on sliding mode method for uncertain linear systems with quantization.

    Science.gov (United States)

    Hao, Li-Ying; Yang, Guang-Hong

    2013-09-01

    This paper is concerned with the problem of robust fault-tolerant compensation control problem for uncertain linear systems subject to both state and input signal quantization. By incorporating novel matrix full-rank factorization technique with sliding surface design successfully, the total failure of certain actuators can be coped with, under a special actuator redundancy assumption. In order to compensate for quantization errors, an adjustment range of quantization sensitivity for a dynamic uniform quantizer is given through the flexible choices of design parameters. Comparing with the existing results, the derived inequality condition leads to the fault tolerance ability stronger and much wider scope of applicability. With a static adjustment policy of quantization sensitivity, an adaptive sliding mode controller is then designed to maintain the sliding mode, where the gain of the nonlinear unit vector term is updated automatically to compensate for the effects of actuator faults, quantization errors, exogenous disturbances and parameter uncertainties without the need for a fault detection and isolation (FDI) mechanism. Finally, the effectiveness of the proposed design method is illustrated via a model of a rocket fairing structural-acoustic.

  17. SK channel modulation rescues striatal plasticity and control over habit in cannabinoid tolerance.

    Science.gov (United States)

    Nazzaro, Cristiano; Greco, Barbara; Cerovic, Milica; Baxter, Paul; Rubino, Tiziana; Trusel, Massimo; Parolaro, Daniela; Tkatch, Tatiana; Benfenati, Fabio; Pedarzani, Paola; Tonini, Raffaella

    2012-01-08

    Endocannabinoids (eCBs) regulate neuronal activity in the dorso-lateral striatum (DLS), a brain region that is involved in habitual behaviors. How synaptic eCB signaling contributes to habitual behaviors under physiological and pathological conditions remains unclear. Using a mouse model of cannabinoid tolerance, we found that persistent activation of the eCB pathway impaired eCB-mediated long-term depression (LTD) and synaptic depotentiation in the DLS. The loss of eCB LTD, occurring preferentially at cortical connections to striatopallidal neurons, was associated with a shift in behavioral control from goal-directed action to habitual responding. eCB LTD and behavioral alterations were rescued by in vivo modulation of small-conductance calcium activated potassium channel (SK channel) activity in the DLS, which potentiates eCB signaling. Our results reveal a direct relationship between drug tolerance and changes in control of instrumental performance by establishing a central role for eCB LTD in habit expression. In addition, SK channels emerge as molecular targets to fine tune the eCB pathway under pathological conditions.

  18. A Framework for Probabilistic Evaluation of Interval Management Tolerance in the Terminal Radar Control Area

    Science.gov (United States)

    Hercencia-Zapana, Heber; Herencia-Zapana, Heber; Hagen, George E.; Neogi, Natasha

    2012-01-01

    Projections of future traffic in the national airspace show that most of the hub airports and their attendant airspace will need to undergo significant redevelopment and redesign in order to accommodate any significant increase in traffic volume. Even though closely spaced parallel approaches increase throughput into a given airport, controller workload in oversubscribed metroplexes is further taxed by these approaches that require stringent monitoring in a saturated environment. The interval management (IM) concept in the TRACON area is designed to shift some of the operational burden from the control tower to the flight deck, placing the flight crew in charge of implementing the required speed changes to maintain a relative spacing interval. The interval management tolerance is a measure of the allowable deviation from the desired spacing interval for the IM aircraft (and its target aircraft). For this complex task, Formal Methods can help to ensure better design and system implementation. In this paper, we propose a probabilistic framework to quantify the uncertainty and performance associated with the major components of the IM tolerance. The analytical basis for this framework may be used to formalize both correctness and probabilistic system safety claims in a modular fashion at the algorithmic level in a way compatible with several Formal Methods tools.

  19. Preferential control of induced regulatory T cell homeostasis via a Bim/Bcl-2 axis.

    Science.gov (United States)

    Wang, X; Szymczak-Workman, A L; Gravano, D M; Workman, C J; Green, D R; Vignali, D A A

    2012-02-09

    Apoptosis has an essential role in controlling T cell homeostasis, especially during the contraction phase of an immune response. However, its contribution to the balance between effector and regulatory populations remains unclear. We found that Rag1(-/-) hosts repopulated with Bim(-/-) conventional CD4(+) T cells (Tconv) resulted in a larger induced regulatory T cell (iTreg) population than mice given wild-type (WT) Tconv. This appears to be due to an increased survival advantage of iTregs compared with activated Tconv in the absence of Bim. Downregulation of Bcl-2 expression and upregulation of Bim expression were more dramatic in WT iTregs than activated Tconv in the absence of IL-2 in vitro. The iTregs generated following Tconv reconstitution of Rag1(-/-) hosts exhibited lower Bcl-2 expression and higher Bim/Bcl-2 ratio than Tconv, which indicates that iTregs were in an apoptosis-prone state in vivo. A significant proportion of the peripheral iTreg pool exhibits low Bcl-2 expression indicating increased sensitivity to apoptosis, which may be a general characteristic of certain Treg subpopulations. In summary, our data suggest that iTregs and Tconv differ in their sensitivity to apoptotic stimuli due to their altered ratio of Bim/Bcl-2 expression. Modulating the apoptosis pathway may provide novel therapeutic approaches to alter the balance between effector T cells and Tregs.

  20. Transcriptional control by two leucine-responsive regulatory proteins in Halobacterium salinarum R1

    Directory of Open Access Journals (Sweden)

    Tarasov Valery

    2010-05-01

    Full Text Available Abstract Background Archaea combine bacterial-as well as eukaryotic-like features to regulate cellular processes. Halobacterium salinarum R1 encodes eight leucine-responsive regulatory protein (Lrp-homologues. The function of two of them, Irp (OE3923F and lrpA1 (OE2621R, were analyzed by gene deletion and overexpression, including genome scale impacts using microarrays. Results It was shown that Lrp affects the transcription of multiple target genes, including those encoding enzymes involved in amino acid synthesis, central metabolism, transport processes and other regulators of transcription. In contrast, LrpA1 regulates transcription in a more specific manner. The aspB3 gene, coding for an aspartate transaminase, was repressed by LrpA1 in the presence of L-aspartate. Analytical DNA-affinity chromatography was adapted to high salt, and demonstrated binding of LrpA1 to its own promoter, as well as L-aspartate dependent binding to the aspB3 promoter. Conclusion The gene expression profiles of two archaeal Lrp-homologues report in detail their role in H. salinarum R1. LrpA1 and Lrp show similar functions to those already described in bacteria, but in addition they play a key role in regulatory networks, such as controlling the transcription of other regulators. In a more detailed analysis ligand dependent binding of LrpA1 was demonstrated to its target gene aspB3.

  1. Comparative Study of Regulatory Circuits in Two Sea Urchin Species Reveals Tight Control of Timing and High Conservation of Expression Dynamics.

    Directory of Open Access Journals (Sweden)

    Tsvia Gildor

    2015-07-01

    Full Text Available Accurate temporal control of gene expression is essential for normal development and must be robust to natural genetic and environmental variation. Studying gene expression variation within and between related species can delineate the level of expression variability that development can tolerate. Here we exploit the comprehensive model of sea urchin gene regulatory networks and generate high-density expression profiles of key regulatory genes of the Mediterranean sea urchin, Paracentrotus lividus (Pl. The high resolution of our studies reveals highly reproducible gene initiation times that have lower variation than those of maximal mRNA levels between different individuals of the same species. This observation supports a threshold behavior of gene activation that is less sensitive to input concentrations. We then compare Mediterranean sea urchin gene expression profiles to those of its Pacific Ocean relative, Strongylocentrotus purpuratus (Sp. These species shared a common ancestor about 40 million years ago and show highly similar embryonic morphologies. Our comparative analyses of five regulatory circuits operating in different embryonic territories reveal a high conservation of the temporal order of gene activation but also some cases of divergence. A linear ratio of 1.3-fold between gene initiation times in Pl and Sp is partially explained by scaling of the developmental rates with temperature. Scaling the developmental rates according to the estimated Sp-Pl ratio and normalizing the expression levels reveals a striking conservation of relative dynamics of gene expression between the species. Overall, our findings demonstrate the ability of biological developmental systems to tightly control the timing of gene activation and relative dynamics and overcome expression noise induced by genetic variation and growth conditions.

  2. Robust control of uncertain nonlinear switched genetic regulatory networks with time delays: A redesign approach.

    Science.gov (United States)

    Moradi, Hojjatullah; Majd, Vahid Johari

    2016-05-01

    In this paper, the problem of robust stability of nonlinear genetic regulatory networks (GRNs) is investigated. The developed method is an integral sliding mode control based redesign for a class of perturbed dissipative switched GRNs with time delays. The control law is redesigned by modifying the dissipativity-based control law that was designed for the unperturbed GRNs with time delays. The switched GRNs are switched from one mode to another based on time, state, etc. Although, the active subsystem is known in any instance, but the switching law and the transition probabilities are not known. The model for each mode is considered affine with matched and unmatched perturbations. The redesigned control law forces the GRN to always remain on the sliding surface and the dissipativity is maintained from the initial time in the presence of the norm-bounded perturbations. The global stability of the perturbed GRNs is maintained if the unperturbed model is globally dissipative. The designed control law for the perturbed GRNs guarantees robust exponential or asymptotic stability of the closed-loop network depending on the type of stability of the unperturbed model. The results are applied to a nonlinear switched GRN, and its convergence to the origin is verified by simulation.

  3. Evaluation of Freezing Tolerance of Three Ajowan (Trachyspermum ammi (Linn. Sprague Ecotypes in Controlled Conditions

    Directory of Open Access Journals (Sweden)

    Z Boroumand Rezazadeh

    2013-08-01

    Full Text Available Ajowan is one of the endemic plants in Khorasan province, and there is a little information on its tolerance to cold stress. In order to study freezing tolerance of ajowan, an experiment was conducted in faculty of agriculture, Ferdowsi University of Mashhad, based on factorial-completely randomized design with three replications and three ecotypes of ajowan (Neishabour, Birjand and Torbat-e-Heidarieh were imposed on eight freezing temperatures (0 (control, -1.5, -3, -4.5, -6,-7.5, -9 and -10.5 °C. Plants were grown in natural environment till 4-5 leaf stage, then for freezing treatments transferred to thermo-gradient freezer. The cell membrane stability was evaluated by electrolyte leakage index (EL and temperature for killing 50% of samples according to the electrolyte leakage (LT50el was determined. Furthermore, survival percentage, leaf number and dry weight, temperature for killing 50% of samples according to survival (LT50su and reduced dry matter temperature 50 (RDMT50 were determined after three weeks recovery in the glasshouse. Response of ajowan ecotypes for electrolyte leakage was different and birjand ecotype had the lowest %EL, whereas the slope of %EL in mentioned ecotype was lower than two other ecotypes. However there were no significant differences among ajowan ecotypes on LT50su. Decreasing temperature to -7.5 °C reduced survival percentage of Neishabour and Torbat-e-Heidarieh ecotypes to lower than 20 percent, whiles in this temperature Birjand’s survival percentage was about 60 percent. It seems that Birjand ecotype with the lowest electrolyte leakage, the highest survival and dry matter and the lowest LT50su was more tolerant than two other ecotypes.

  4. Hybridization of an invasive shrub affects tolerance and resistance to defoliation by a biological control agent

    Science.gov (United States)

    Williams, Wyatt I.; Friedman, Jonathan M.; Gaskin, John F.; Norton, Andrew P.

    2014-01-01

    Evolution has contributed to the successful invasion of exotic plant species in their introduced ranges, but how evolution affects particular control strategies is still under evaluation. For instance, classical biological control, a common strategy involving the utilization of highly specific natural enemies to control exotic pests, may be negatively affected by host hybridization because of shifts in plant traits, such as root allocation or chemical constituents. We investigated introgression between two parent species of the invasive shrub tamarisk (Tamarix spp.) in the western United States, and how differences in plant traits affect interactions with a biological control agent. Introgression varied strongly with latitude of origin and was highly correlated with plant performance. Increased levels of T. ramosissima introgression resulted in both higher investment in roots and tolerance to defoliation and less resistance to insect attack. Because tamarisk hybridization occurs predictably on the western U.S. landscape, managers may be able to exploit this information to maximize control efforts. Genetic differentiation in plant traits in this system underpins the importance of plant hybridization and may explain why some biological control releases are more successful than others.

  5. Fault tolerant attitude sensing and force feedback control for unmanned aerial vehicles

    Science.gov (United States)

    Jagadish, Chirag

    Two aspects of an unmanned aerial vehicle are studied in this work. One is fault tolerant attitude determination and the other is to provide force feedback to the joy-stick of the UAV so as to prevent faulty inputs from the pilot. Determination of attitude plays an important role in control of aerial vehicles. One way of defining the attitude is through Euler angles. These angles can be determined based on the measurements of the projections of the gravity and earth magnetic fields on the three body axes of the vehicle. Attitude determination in unmanned aerial vehicles poses additional challenges due to limitations of space, payload, power and cost. Therefore it provides for almost no room for any bulky sensors or extra sensor hardware for backup and as such leaves no room for sensor fault issues either. In the face of these limitations, this study proposes a fault tolerant computing of Euler angles by utilizing multiple different computation methods, with each method utilizing a different subset of the available sensor measurement data. Twenty-five such methods have been presented in this document. The capability of computing the Euler angles in multiple ways provides a diversified redundancy required for fault tolerance. The proposed approach can identify certain sets of sensor failures and even separate the reference fields from the disturbances. A bank-to-turn maneuver of the NASA GTM UAV is used to demonstrate the fault tolerance provided by the proposed method as well as to demonstrate the method of determining the correct Euler angles despite interferences by inertial acceleration disturbances. Attitude computation is essential for stability. But as of today most UAVs are commanded remotely by human pilots. While basic stability control is entrusted to machine or the on-board automatic controller, overall guidance is usually with humans. It is therefore the pilot who sets the command/references through a joy-stick. While this is a good compromise between

  6. Fault Tolerance Automotive Air-Ratio Control Using Extreme Learning Machine Model Predictive Controller

    OpenAIRE

    Pak Kin Wong; Hang Cheong Wong; Chi Man Vong; Tong Meng Iong; Ka In Wong; Xianghui Gao

    2015-01-01

    Effective air-ratio control is desirable to maintain the best engine performance. However, traditional air-ratio control assumes the lambda sensor located at the tail pipe works properly and relies strongly on the air-ratio feedback signal measured by the lambda sensor. When the sensor is warming up during cold start or under failure, the traditional air-ratio control no longer works. To address this issue, this paper utilizes an advanced modelling technique, kernel extreme learning machine (...

  7. Comparing genomes to computer operating systems in terms of the topology and evolution of their regulatory control networks.

    Science.gov (United States)

    Yan, Koon-Kiu; Fang, Gang; Bhardwaj, Nitin; Alexander, Roger P; Gerstein, Mark

    2010-05-18

    The genome has often been called the operating system (OS) for a living organism. A computer OS is described by a regulatory control network termed the call graph, which is analogous to the transcriptional regulatory network in a cell. To apply our firsthand knowledge of the architecture of software systems to understand cellular design principles, we present a comparison between the transcriptional regulatory network of a well-studied bacterium (Escherichia coli) and the call graph of a canonical OS (Linux) in terms of topology and evolution. We show that both networks have a fundamentally hierarchical layout, but there is a key difference: The transcriptional regulatory network possesses a few global regulators at the top and many targets at the bottom; conversely, the call graph has many regulators controlling a small set of generic functions. This top-heavy organization leads to highly overlapping functional modules in the call graph, in contrast to the relatively independent modules in the regulatory network. We further develop a way to measure evolutionary rates comparably between the two networks and explain this difference in terms of network evolution. The process of biological evolution via random mutation and subsequent selection tightly constrains the evolution of regulatory network hubs. The call graph, however, exhibits rapid evolution of its highly connected generic components, made possible by designers' continual fine-tuning. These findings stem from the design principles of the two systems: robustness for biological systems and cost effectiveness (reuse) for software systems.

  8. The Regulatory Easy Street: Self-Regulation Below the Self-Control Threshold Does not Consume Regulatory Resources.

    Science.gov (United States)

    Vandellen, Michelle R; Hoyle, Rick H; Miller, Rebecca

    2012-06-01

    We present and test a theory in which self-control is distinguished from broader acts of self-regulation when it is both effortful and conscious. In two studies, we examined whether acts of behavioral management that do not require effort are exempt from resource depletion. In Study 1, we found that a self-regulation task only reduced subsequent self-control for participants who had previously indicated that completing the task would require effort. In Study 2, we found that participants who completed a self-regulation task for two minutes did not evidence the subsequent impairment in self-control evident for participants who had completed the task for four or more minutes. Our results support the notion that self-regulation without effort falls below the self-control threshold and has different downstream consequences than self-control.

  9. Nuclear Forensic Science: Analysis of Nuclear Material Out of Regulatory Control

    Science.gov (United States)

    Kristo, Michael J.; Gaffney, Amy M.; Marks, Naomi; Knight, Kim; Cassata, William S.; Hutcheon, Ian D.

    2016-06-01

    Nuclear forensic science seeks to identify the origin of nuclear materials found outside regulatory control. It is increasingly recognized as an integral part of a robust nuclear security program. This review highlights areas of active, evolving research in nuclear forensics, with a focus on analytical techniques commonly employed in Earth and planetary sciences. Applications of nuclear forensics to uranium ore concentrates (UOCs) are discussed first. UOCs have become an attractive target for nuclear forensic researchers because of the richness in impurities compared to materials produced later in the fuel cycle. The development of chronometric methods for age dating nuclear materials is then discussed, with an emphasis on improvements in accuracy that have been gained from measurements of multiple radioisotopic systems. Finally, papers that report on casework are reviewed, to provide a window into current scientific practice.

  10. Media ion composition controls regulatory and virulence response of Salmonella in spaceflight.

    Directory of Open Access Journals (Sweden)

    James W Wilson

    Full Text Available The spaceflight environment is relevant to conditions encountered by pathogens during the course of infection and induces novel changes in microbial pathogenesis not observed using conventional methods. It is unclear how microbial cells sense spaceflight-associated changes to their growth environment and orchestrate corresponding changes in molecular and physiological phenotypes relevant to the infection process. Here we report that spaceflight-induced increases in Salmonella virulence are regulated by media ion composition, and that phosphate ion is sufficient to alter related pathogenesis responses in a spaceflight analogue model. Using whole genome microarray and proteomic analyses from two independent Space Shuttle missions, we identified evolutionarily conserved molecular pathways in Salmonella that respond to spaceflight under all media compositions tested. Identification of conserved regulatory paradigms opens new avenues to control microbial responses during the infection process and holds promise to provide an improved understanding of human health and disease on Earth.

  11. Data control and digital regulatory space(s: towards a new European approach

    Directory of Open Access Journals (Sweden)

    Roxana Radu

    2015-06-01

    Full Text Available Data control, among the newest forms of power fostered by information and communication technologies (ICTs, triggers a continuous (renegotiation of public and private orderings, with direct implications on both regulators and intermediaries. This article examines the stance of the European Union (EU regarding the position of Google - the world’s largest internet services company as per its 2014 market value - in two controversial instances: the ‘right to be forgotten’ and the implementation of EU competition rules. It provides an analysis of these evolving debates and their meaning for EU regulatory thrust more broadly, discussing the shift in the approach to digital markets and the proactive development of a European framework influential beyond continental boundaries.

  12. Small regulatory RNAs control the multi-cellular adhesive lifestyle of Escherichia coli

    DEFF Research Database (Denmark)

    Jørgensen, Mikkel Girke; Nielsen, Jesper Sejrup; Boysen, Anders;

    2012-01-01

    . Our demonstration that basal expression of each of the three RNA species is sufficient to downregulate CsgD synthesis and prevent curli formation indicates that all play a prominent role in the curli regulatory network. Our findings provide the first clue as to how the Rcs signalling pathway...... and adhesive state that enables biofilm formation on surfaces. For this, the bacterium needs to reprogramme its gene expression, and in many E. coli and Salmonella strains the lifestyle shift relies on control cascades that inhibit flagellar expression and activate the synthesis of curli, extracellular...... adhesive fibres important for co-aggregation of cells and adhesion to biotic and abiotic surfaces. By combining bioinformatics, genetic and biochemical analysis we identified three small RNAs that act by an antisense mechanism to downregulate translation of CsgD, the master regulator of curli synthesis...

  13. Calcineurin controls drug tolerance, hyphal growth, and virulence in Candida dubliniensis.

    Science.gov (United States)

    Chen, Ying-Lien; Brand, Alexandra; Morrison, Emma L; Silao, Fitz Gerald S; Bigol, Ursela G; Malbas, Fedelino F; Nett, Jeniel E; Andes, David R; Solis, Norma V; Filler, Scott G; Averette, Anna; Heitman, Joseph

    2011-06-01

    Candida dubliniensis is an emerging pathogenic yeast species closely related to Candida albicans and frequently found colonizing or infecting the oral cavities of HIV/AIDS patients. Drug resistance during C. dubliniensis infection is common and constitutes a significant therapeutic challenge. The calcineurin inhibitor FK506 exhibits synergistic fungicidal activity with azoles or echinocandins in the fungal pathogens C. albicans, Cryptococcus neoformans, and Aspergillus fumigatus. In this study, we show that calcineurin is required for cell wall integrity and wild-type tolerance of C. dubliniensis to azoles and echinocandins; hence, these drugs are candidates for combination therapy with calcineurin inhibitors. In contrast to C. albicans, in which the roles of calcineurin and Crz1 in hyphal growth are unclear, here we show that calcineurin and Crz1 play a clearly demonstrable role in hyphal growth in response to nutrient limitation in C. dubliniensis. We further demonstrate that thigmotropism is controlled by Crz1, but not calcineurin, in C. dubliniensis. Similar to C. albicans, C. dubliniensis calcineurin enhances survival in serum. C. dubliniensis calcineurin and crz1/crz1 mutants exhibit attenuated virulence in a murine systemic infection model, likely attributable to defects in cell wall integrity, hyphal growth, and serum survival. Furthermore, we show that C. dubliniensis calcineurin mutants are unable to establish murine ocular infection or form biofilms in a rat denture model. That calcineurin is required for drug tolerance and virulence makes fungus-specific calcineurin inhibitors attractive candidates for combination therapy with azoles or echinocandins against emerging C. dubliniensis infections.

  14. A Hybrid Sensor Based Backstepping Control Approach with its Application to Fault-Tolerant Flight Control

    NARCIS (Netherlands)

    Sun, L.G.; De Visser, C.C.; Chu, Q.P.; Falkena, W.

    2013-01-01

    Recently, an incremental type sensor based backstepping (SBB) control approach, based on singular perturbation theory and Tikhonov’s theorem, has been proposed. This Lyapunov function based method uses measurements of control variables and less model knowledge, and it is not susceptible to the model

  15. A Hybrid Sensor Based Backstepping Control Approach with its Application to Fault-Tolerant Flight Control

    NARCIS (Netherlands)

    Sun, L.G.; De Visser, C.C.; Chu, Q.P.; Falkena, W.

    2013-01-01

    Recently, an incremental type sensor based backstepping (SBB) control approach, based on singular perturbation theory and Tikhonov’s theorem, has been proposed. This Lyapunov function based method uses measurements of control variables and less model knowledge, and it is not susceptible to the model

  16. Evaluation of Freeze Tolerance in Lancelot Plantain (Plantago lanceolata L. Ecotypes under Controlled Conditions

    Directory of Open Access Journals (Sweden)

    M. Janalizadeh

    2016-02-01

    Full Text Available Introduction Lancelot Plantain (Ribwort, narrow-leaf or English plantain is a deep-rooted, short-lived perennial herb from Plantaginaceae family which has been used for various medicinal purposes for centuries, especially in Europe and only more recently has been proposed as a forage plant. The leaf of plantain is highly palatable for grazing animals, providing mineral-rich forage. Recently two productive upright cultivars of plantain have been bred and introduced, Grasslands Lancelot and the more erect winter active Ceres Tonic. Plantain grows moderately in winter but its main growth periods beings in spring and autumn with opportunistic summer growth. Although it reveals suitable winter survival in natural conditions, but there is not a lot of information about cold tolerance of this plant. So it is important to recognize the freeze tolerance of narrow leaf plantain for successful planting and utilization in cold regions such as Mashhad in Khorasan Razavi Province (Northeast of Iran. Determining LT50 point or critical temperature for survival of plant is the most reliable and simple method for evaluating cold tolerance of plants. Another reliable method for freeze tolerance of plants is estimation of temperature at which 50 % of dry matter reduces (RDMT50. This experiment was carried out to evaluate freeze tolerance of five ecotypes of Lancelot plantain according to the LT50su and RDMT50 indices. Materials and Methods In order to evaluate freeze tolerance of Lancelot plantain, a factorial experiment based on completely randomized design with three replications was carried out under controlled conditions at college of agriculture, Ferdowsi University of Mashhad. Five ecotypes of Lancelot plantain (Bojnourd, Kalat, Mashhad, Ghayen and Birjand after three months growth and hardening in natural environment were transferred to a Thermo gradient freezer on January 20th, 2012 and exposed to eight freezing temperatures (Zero, -3, -6, -9, -12, -15, -18

  17. Positive lists of cosmetic ingredients: Analytical methodology for regulatory and safety controls – A review

    Energy Technology Data Exchange (ETDEWEB)

    Lores, Marta, E-mail: marta.lores@usc.es; Llompart, Maria; Alvarez-Rivera, Gerardo; Guerra, Eugenia; Vila, Marlene; Celeiro, Maria; Lamas, J. Pablo; Garcia-Jares, Carmen

    2016-04-07

    Cosmetic products placed on the market and their ingredients, must be safe under reasonable conditions of use, in accordance to the current legislation. Therefore, regulated and allowed chemical substances must meet the regulatory criteria to be used as ingredients in cosmetics and personal care products, and adequate analytical methodology is needed to evaluate the degree of compliance. This article reviews the most recent methods (2005–2015) used for the extraction and the analytical determination of the ingredients included in the positive lists of the European Regulation of Cosmetic Products (EC 1223/2009): comprising colorants, preservatives and UV filters. It summarizes the analytical properties of the most relevant analytical methods along with the possibilities of fulfilment of the current regulatory issues. The cosmetic legislation is frequently being updated; consequently, the analytical methodology must be constantly revised and improved to meet safety requirements. The article highlights the most important advances in analytical methodology for cosmetics control, both in relation to the sample pretreatment and extraction and the different instrumental approaches developed to solve this challenge. Cosmetics are complex samples, and most of them require a sample pretreatment before analysis. In the last times, the research conducted covering this aspect, tended to the use of green extraction and microextraction techniques. Analytical methods were generally based on liquid chromatography with UV detection, and gas and liquid chromatographic techniques hyphenated with single or tandem mass spectrometry; but some interesting proposals based on electrophoresis have also been reported, together with some electroanalytical approaches. Regarding the number of ingredients considered for analytical control, single analyte methods have been proposed, although the most useful ones in the real life cosmetic analysis are the multianalyte approaches. - Highlights:

  18. Positive lists of cosmetic ingredients: Analytical methodology for regulatory and safety controls - A review.

    Science.gov (United States)

    Lores, Marta; Llompart, Maria; Alvarez-Rivera, Gerardo; Guerra, Eugenia; Vila, Marlene; Celeiro, Maria; Lamas, J Pablo; Garcia-Jares, Carmen

    2016-04-07

    Cosmetic products placed on the market and their ingredients, must be safe under reasonable conditions of use, in accordance to the current legislation. Therefore, regulated and allowed chemical substances must meet the regulatory criteria to be used as ingredients in cosmetics and personal care products, and adequate analytical methodology is needed to evaluate the degree of compliance. This article reviews the most recent methods (2005-2015) used for the extraction and the analytical determination of the ingredients included in the positive lists of the European Regulation of Cosmetic Products (EC 1223/2009): comprising colorants, preservatives and UV filters. It summarizes the analytical properties of the most relevant analytical methods along with the possibilities of fulfilment of the current regulatory issues. The cosmetic legislation is frequently being updated; consequently, the analytical methodology must be constantly revised and improved to meet safety requirements. The article highlights the most important advances in analytical methodology for cosmetics control, both in relation to the sample pretreatment and extraction and the different instrumental approaches developed to solve this challenge. Cosmetics are complex samples, and most of them require a sample pretreatment before analysis. In the last times, the research conducted covering this aspect, tended to the use of green extraction and microextraction techniques. Analytical methods were generally based on liquid chromatography with UV detection, and gas and liquid chromatographic techniques hyphenated with single or tandem mass spectrometry; but some interesting proposals based on electrophoresis have also been reported, together with some electroanalytical approaches. Regarding the number of ingredients considered for analytical control, single analyte methods have been proposed, although the most useful ones in the real life cosmetic analysis are the multianalyte approaches. Copyright © 2016

  19. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents

    Directory of Open Access Journals (Sweden)

    J.B. Calixto

    2000-02-01

    Full Text Available This review highlights the current advances in knowledge about the safety, efficacy, quality control, marketing and regulatory aspects of botanical medicines. Phytotherapeutic agents are standardized herbal preparations consisting of complex mixtures of one or more plants which contain as active ingredients plant parts or plant material in the crude or processed state. A marked growth in the worldwide phytotherapeutic market has occurred over the last 15 years. For the European and USA markets alone, this will reach about $7 billion and $5 billion per annum, respectively, in 1999, and has thus attracted the interest of most large pharmaceutical companies. Insufficient data exist for most plants to guarantee their quality, efficacy and safety. The idea that herbal drugs are safe and free from side effects is false. Plants contain hundreds of constituents and some of them are very toxic, such as the most cytotoxic anti-cancer plant-derived drugs, digitalis and the pyrrolizidine alkaloids, etc. However, the adverse effects of phytotherapeutic agents are less frequent compared with synthetic drugs, but well-controlled clinical trials have now confirmed that such effects really exist. Several regulatory models for herbal medicines are currently available including prescription drugs, over-the-counter substances, traditional medicines and dietary supplements. Harmonization and improvement in the processes of regulation is needed, and the general tendency is to perpetuate the German Commission E experience, which combines scientific studies and traditional knowledge (monographs. Finally, the trend in the domestication, production and biotechnological studies and genetic improvement of medicinal plants, instead of the use of plants harvested in the wild, will offer great advantages, since it will be possible to obtain uniform and high quality raw materials which are fundamental to the efficacy and safety of herbal drugs.

  20. Supervisory Fault Tolerant Control of the GTM UAV Using LPV Methods

    Directory of Open Access Journals (Sweden)

    Péni Tamás

    2015-03-01

    Full Text Available A multi-level reconfiguration framework is proposed for fault tolerant control of over-actuated aerial vehicles, where the levels indicate how much authority is given to the reconfiguration task. On the lowest, first level the fault is accommodated by modifying only the actuator/sensor configuration, so the fault remains hidden from the baseline controller. A dynamic reallocation scheme is applied on this level. The allocation mechanism exploits the actuator/sensor redundancy available on the aircraft. When the fault cannot be managed at the actuator/sensor level, the reconfiguration process has access to the baseline controller. Based on the LPV control framework, this is done by introducing fault-specific scheduling parameters. The baseline controller is designed to provide an acceptable performance level along all fault scenarios coded in these scheduling variables. The decision on which reconfiguration level has to be initiated in response to a fault is determined by a supervisor unit. The method is demonstrated on a full six-degrees-of-freedom nonlinear simulation model of the GTM UAV.

  1. Flight Tests of Autopilot Integrated with Fault-Tolerant Control of a Small Fixed-Wing UAV

    Directory of Open Access Journals (Sweden)

    Shuo Wang

    2016-01-01

    Full Text Available A fault-tolerant control scheme for the autopilot of the small fixed-wing UAV is designed and tested by the actual flight experiments. The small fixed-wing UAV called Xiang Fei is developed independently by Nanjing University of Aeronautics and Astronautics. The flight control system is designed based on an open-source autopilot (Pixhawk. Real-time kinematic (RTK GPS is introduced due to its high accuracy. Some modifications on the longitudinal and lateral guidance laws are achieved to improve the flight control performance. Moreover, a data fusion based fault-tolerant control scheme is integrated in altitude control and speed control for altitude sensor failure and airspeed sensor failure, which are the common problems for small fixed-wing UAV. Finally, the real flight experiments are implemented to test the fault-tolerant control based autopilot of UAV. Real flight test results are given and analyzed in detail, which show that the fixed-wing UAV can track the desired altitude and speed commands during the whole flight process including takeoff, climbing, cruising, gliding, landing, and wave-off by the fault-tolerant control based autopilot.

  2. Genotypic difference in salinity tolerance in quinoa is determined by differential control of xylem Na(+) loading and stomatal density.

    Science.gov (United States)

    Shabala, Sergey; Hariadi, Yuda; Jacobsen, Sven-Erik

    2013-07-01

    Quinoa is regarded as a highly salt tolerant halophyte crop, of great potential for cultivation on saline areas around the world. Fourteen quinoa genotypes of different geographical origin, differing in salinity tolerance, were grown under greenhouse conditions. Salinity treatment started on 10 day old seedlings. Six weeks after the treatment commenced, leaf sap Na and K content and osmolality, stomatal density, chlorophyll fluorescence characteristics, and xylem sap Na and K composition were measured. Responses to salinity differed greatly among the varieties. All cultivars had substantially increased K(+) concentrations in the leaf sap, but the most tolerant cultivars had lower xylem Na(+) content at the time of sampling. Most tolerant cultivars had lowest leaf sap osmolality. All varieties reduced stomata density when grown under saline conditions. All varieties clustered into two groups (includers and excluders) depending on their strategy of handling Na(+) under saline conditions. Under control (non-saline) conditions, a strong positive correlation was observed between salinity tolerance and plants ability to accumulate Na(+) in the shoot. Increased leaf sap K(+), controlled Na(+) loading to the xylem, and reduced stomata density are important physiological traits contributing to genotypic differences in salinity tolerance in quinoa, a halophyte species from Chenopodium family.

  3. Fault-tolerant strategies for an implantable centrifugal blood pump using a radially controlled magnetic bearing.

    Science.gov (United States)

    Pai, Chi Nan; Shinshi, Tadahiko

    2011-10-01

    In our laboratory, an implantable centrifugal blood pump (CBP) with a two degrees-of-freedom radially controlled magnetic bearing (MB) to support the impeller without contact has been developed to assist the pumping function of the weakened heart ventricle. In order to maintain the function of the CBP after damage to the electromagnets (EMs) of the MB, fault-tolerant strategies for the CBP are proposed in this study. Using a redundant MB design, magnetic levitation of the impeller was maintained with damage to up to two out of a total of four EMs of the MB; with damage to three EMs, contact-free support of the impeller was achieved using hydrodynamic and electromagnetic forces; and with damage to all four EMs, the pump operating point, of 5 l/min against 100 mmHg, was achieved using the motor for rotation of the impeller, with contact between the impeller and the stator.

  4. Data-based fault-tolerant control for affine nonlinear systems with actuator faults.

    Science.gov (United States)

    Xie, Chun-Hua; Yang, Guang-Hong

    2016-09-01

    This paper investigates the fault-tolerant control (FTC) problem for unknown nonlinear systems with actuator faults including stuck, outage, bias and loss of effectiveness. The upper bounds of stuck faults, bias faults and loss of effectiveness faults are unknown. A new data-based FTC scheme is proposed. It consists of the online estimations of the bounds and a state-dependent function. The estimations are adjusted online to compensate automatically the actuator faults. The state-dependent function solved by using real system data helps to stabilize the system. Furthermore, all signals in the resulting closed-loop system are uniformly bounded and the states converge asymptotically to zero. Compared with the existing results, the proposed approach is data-based. Finally, two simulation examples are provided to show the effectiveness of the proposed approach.

  5. Regulatory Research of the PWR Severe Accident. Information Needs and Instrumentation for Hydrogen Control and Management

    Energy Technology Data Exchange (ETDEWEB)

    Park, Gun Chul; Suh, Kune Y.; Lee, Jin Yong; Lee, Seung Dong [Seoul Nat' l Univ., Seoul (Korea, Republic of)

    2001-03-15

    The current research is concerned with generation of basic engineering data needed in the process of developing hydrogen control guidelines as part of accident management strategies for domestic nuclear power plants and formulating pertinent regulatory requirements. Major focus is placed on identification of information needs and instrumentation methods for hydrogen control and management in the primary system and in the containment, development of decision-making trees for hydrogen management and their quantification, the instrument availability under severe accident conditions, critical review of relevant hydrogen generation model and phenomena In relation to hydrogen behavior, we analyzed the severe accident related hydrogen generation in the UCN 3{center_dot}4 PWR with modified hydrogen generation model. On the basis of the hydrogen mixing experiment and related GASFLOW calculation, the necessity of 3-dimensional analysis of the hydrogen mixing was investigated. We examined the hydrogen control models related to the PAR(Passive Autocatalytic Recombiner) and performed MAAP4 calculation in relation to the decision tree to estimate the capability and the role of the PAR during a severe accident.

  6. Guaranteed Cost Fault-tolerant Control of Networked Control Systems with Short Output Delay and Short Control Delay Based on State Observer

    Directory of Open Access Journals (Sweden)

    Xiaomao Huang

    2013-04-01

    Full Text Available Supposing that the sensor and controller nodes were time-driven and the actuator node was event-driven, the problem of integrity against sensor failures for the networked control systems with short output delay and short control delay was discussed based on observer. The state observer of the system according to the time-delay compensation strategy was designed. Then, considering possible sensor failures, an augmented mathematic model for the networked control systems based on observer was developed. In terms of the given quadratic performance index function, the integrity condition of the system was given and the designs for guaranteed cost fault-tolerant controller and observer were presented respectively by using the cooperative design approach of the controller and observer and the approach of bilinear matrix inequalities. Finally, a numerical simulation example demonstrated the conclusions are feasible and effective. The proposed control method meets the requirements in industrial networked control systems.

  7. A review of fault tolerant control strategies applied to proton exchange membrane fuel cell systems

    Science.gov (United States)

    Dijoux, Etienne; Steiner, Nadia Yousfi; Benne, Michel; Péra, Marie-Cécile; Pérez, Brigitte Grondin

    2017-08-01

    Fuel cells are powerful systems for power generation. They have a good efficiency and do not generate greenhouse gases. This technology involves a lot of scientific fields, which leads to the appearance of strongly inter-dependent parameters. This makes the system particularly hard to control and increases fault's occurrence frequency. These two issues call for the necessity to maintain the system performance at the expected level, even in faulty operating conditions. It is called ;fault tolerant control; (FTC). The present paper aims to give the state of the art of FTC applied to the proton exchange membrane fuel cell (PEMFC). The FTC approach is composed of two parts. First, a diagnosis part allows the identification and the isolation of a fault; it requires a good a priori knowledge of all the possible faults. Then, a control part allows an optimal control strategy to find the best operating point to recover/mitigate the fault; it requires the knowledge of the degradation phenomena and their mitigation strategies.

  8. Fault-tolerant control with mixed aerodynamic surfaces and RCS jets for hypersonic reentry vehicles

    Directory of Open Access Journals (Sweden)

    Jingjing He

    2017-04-01

    Full Text Available This paper proposes a fault-tolerant strategy for hypersonic reentry vehicles with mixed aerodynamic surfaces and reaction control systems (RCS under external disturbances and subject to actuator faults. Aerodynamic surfaces are treated as the primary actuator in normal situations, and they are driven by a continuous quadratic programming (QP allocator to generate torque commanded by a nonlinear adaptive feedback control law. When aerodynamic surfaces encounter faults, they may not be able to provide sufficient torque as commanded, and RCS jets are activated to augment the aerodynamic surfaces to compensate for insufficient torque. Partial loss of effectiveness and stuck faults are considered in this paper, and observers are designed to detect and identify the faults. Based on the fault identification results, an RCS control allocator using integer linear programming (ILP techniques is designed to determine the optimal combination of activated RCS jets. By treating the RCS control allocator as a quantization element, closed-loop stability with both continuous and quantized inputs is analyzed. Simulation results verify the effectiveness of the proposed method.

  9. Fault diagnosis and fault-tolerant control strategies for non-linear systems analytical and soft computing approaches

    CERN Document Server

    Witczak, Marcin

    2014-01-01

      This book presents selected fault diagnosis and fault-tolerant control strategies for non-linear systems in a unified framework. In particular, starting from advanced state estimation strategies up to modern soft computing, the discrete-time description of the system is employed Part I of the book presents original research results regarding state estimation and neural networks for robust fault diagnosis. Part II is devoted to the presentation of integrated fault diagnosis and fault-tolerant systems. It starts with a general fault-tolerant control framework, which is then extended by introducing robustness with respect to various uncertainties. Finally, it is shown how to implement the proposed framework for fuzzy systems described by the well-known Takagi–Sugeno models. This research monograph is intended for researchers, engineers, and advanced postgraduate students in control and electrical engineering, computer science,as well as mechanical and chemical engineering.

  10. Adaptive fault-tolerant control of linear time-invariant systems in the presence of actuator saturation

    Institute of Scientific and Technical Information of China (English)

    Wei GUAN; Guanghong YANG

    2009-01-01

    This paper studies the problem of designing adaptive fault-tolerant controllers for linear time-invariant systems with actuator saturation.New methods for designing indirect adaptive fault-tolerant controllers via state feedback are presented for actuator fault compensations.Based on the on-line estimation of eventual faults,the adaptive fault-tolerant controller parameters are updating automatically to compensate the fault effects on systems.The designs are developed in the framework of linear matrix inequality (LMI) approach,which can enlarge the domain of attraction of closed-loop systems in the cases of actuator saturation and actuator failures.Two examples are given to illustrate the effectiveness of the design method.

  11. Fault tolerant synchronization of chaotic heavy symmetric gyroscope systems versus external disturbances via Lyapunov rule-based fuzzy control.

    Science.gov (United States)

    Farivar, Faezeh; Shoorehdeli, Mahdi Aliyari

    2012-01-01

    In this paper, fault tolerant synchronization of chaotic gyroscope systems versus external disturbances via Lyapunov rule-based fuzzy control is investigated. Taking the general nature of faults in the slave system into account, a new synchronization scheme, namely, fault tolerant synchronization, is proposed, by which the synchronization can be achieved no matter whether the faults and disturbances occur or not. By making use of a slave observer and a Lyapunov rule-based fuzzy control, fault tolerant synchronization can be achieved. Two techniques are considered as control methods: classic Lyapunov-based control and Lyapunov rule-based fuzzy control. On the basis of Lyapunov stability theory and fuzzy rules, the nonlinear controller and some generic sufficient conditions for global asymptotic synchronization are obtained. The fuzzy rules are directly constructed subject to a common Lyapunov function such that the error dynamics of two identical chaotic motions of symmetric gyros satisfy stability in the Lyapunov sense. Two proposed methods are compared. The Lyapunov rule-based fuzzy control can compensate for the actuator faults and disturbances occurring in the slave system. Numerical simulation results demonstrate the validity and feasibility of the proposed method for fault tolerant synchronization.

  12. Reconfigurable fault-tolerant controller synthesis for a steer-by-wire vehicle using independently driven wheels

    Science.gov (United States)

    Wada, Nobutaka; Fujii, Kosuke; Saeki, Masami

    2013-09-01

    In this paper, a synthesis method for a reconfigurable fault-tolerant control system for use in a steer-by-wire vehicle is proposed. The vehicle considered in this paper is also assumed to have independently driven wheels. The control objective in this work is to enable the vehicle yaw rate to track the reference signal even when the steering actuator breaks down. Since the vehicle yaw rate can be controlled with either the front wheel turn angle or the yaw moment generated by the independently driven wheels, this system has actuator redundancy. We attempt to design a control system that manages this actuator redundancy so that the performance degradation due to the actuator failure is minimised. We utilise a control allocator based on on-line optimisation for managing the actuator redundancy. The fault-tolerant control system with a control allocator has several excellent properties. For example, the method can handle various failure situations. Also, since the control allocation problem is reduced to a convex quadratic programming problem, the on-line computational effort is relatively little. However, so far, it has been unclear whether the stability of the control system with the control allocator is guaranteed when the actuator failure occurs. Therefore, we propose a design method of a fault-tolerant controller based on on-line optimisation that guarantees the stability of the overall system. The effectiveness of the method is established through numerical examples.

  13. Association Between Cardiorespiratory Fitness and the Determinants of Glycemic Control Across the Entire Glucose Tolerance Continuum

    DEFF Research Database (Denmark)

    Solomon, Thomas P. J.; Malin, Steven K.; Karstoft, Kristian

    2015-01-01

    OBJECTIVE: Cardiorespiratory fitness (VO2max) is associated with glycemic control, yet the relationship between VO2max and the underlying determinants of glycemic control is less clear. Our aim was to determine whether VO2max is associated with insulin sensitivity, insulin secretion...... underwent measurements of body composition, HbA1c, fasting glucose, oral glucose tolerance (OGTT), and VO2max. OGTT-derived insulin sensitivity (SiOGTT), glucose-stimulated insulin secretion (GSISOGTT), and the disposition index (DIOGTT) (the product of SiOGTT and GSISOGTT) were measured, and associations...... between VO2max and these determinants of glycemic control were examined. RESULTS: A low VO2max was associated with high HbA1c (r = -0.33), high fasting glucose (r = -0.34), high 2-h OGTT glucose (r = -0.33), low SiOGTT (r = 0.73), and high early-phase (r = -0.34) and late-phase (r = -0.36) GSISOGTT...

  14. The GBT-SCA, a radiation tolerant ASIC for detector control applications in SLHC experiments

    CERN Document Server

    Gabrielli, A; Kloukinas, K; Marchioro, A; Moreira, P; Ranieri, A; De Robertis, D

    2009-01-01

    This work describes the architecture of the GigaBit Transceiver – Slow Control Adapter (GBT–SCA) ASIC suitable for the control and monitoring applications of the embedded front-end electronics in the future SLHC experiments. The GBT–SCA is part the GBT chipset currently under development for the SLHC detector upgrades. It is designed for radiation tolerance and it will be fabricated in a commercial 130 nm CMOS technology. The paper discusses the GBT-SCA architecture, the data transfer protocol, the ASIC interfaces, and its integration with the GBT optical link. The GBT–SCA is one the components of the GBT system chipset. It is proposed for the future SLHC experiments and is designed to be configurable matching different front-end system requirements. The GBT-SCA is intended for the slow control and monitoring of the embedded front end electronics and implements a point-to-multi point connection between one GBT optical link ASIC and several front end ASICs. The GBT-SCA connects to a dedicated electrica...

  15. Closed-Loop HIRF Experiments Performed on a Fault Tolerant Flight Control Computer

    Science.gov (United States)

    Belcastro, Celeste M.

    1997-01-01

    ABSTRACT Closed-loop HIRF experiments were performed on a fault tolerant flight control computer (FCC) at the NASA Langley Research Center. The FCC used in the experiments was a quad-redundant flight control computer executing B737 Autoland control laws. The FCC was placed in one of the mode-stirred reverberation chambers in the HIRF Laboratory and interfaced to a computer simulation of the B737 flight dynamics, engines, sensors, actuators, and atmosphere in the Closed-Loop Systems Laboratory. Disturbances to the aircraft associated with wind gusts and turbulence were simulated during tests. Electrical isolation between the FCC under test and the simulation computer was achieved via a fiber optic interface for the analog and discrete signals. Closed-loop operation of the FCC enabled flight dynamics and atmospheric disturbances affecting the aircraft to be represented during tests. Upset was induced in the FCC as a result of exposure to HIRF, and the effect of upset on the simulated flight of the aircraft was observed and recorded. This paper presents a description of these closed- loop HIRF experiments, upset data obtained from the FCC during these experiments, and closed-loop effects on the simulated flight of the aircraft.

  16. Finite Time Fault Tolerant Control for Robot Manipulators Using Time Delay Estimation and Continuous Nonsingular Fast Terminal Sliding Mode Control.

    Science.gov (United States)

    Van, Mien; Ge, Shuzhi Sam; Ren, Hongliang

    2016-04-28

    In this paper, a novel finite time fault tolerant control (FTC) is proposed for uncertain robot manipulators with actuator faults. First, a finite time passive FTC (PFTC) based on a robust nonsingular fast terminal sliding mode control (NFTSMC) is investigated. Be analyzed for addressing the disadvantages of the PFTC, an AFTC are then investigated by combining NFTSMC with a simple fault diagnosis scheme. In this scheme, an online fault estimation algorithm based on time delay estimation (TDE) is proposed to approximate actuator faults. The estimated fault information is used to detect, isolate, and accommodate the effect of the faults in the system. Then, a robust AFTC law is established by combining the obtained fault information and a robust NFTSMC. Finally, a high-order sliding mode (HOSM) control based on super-twisting algorithm is employed to eliminate the chattering. In comparison to the PFTC and other state-of-the-art approaches, the proposed AFTC scheme possess several advantages such as high precision, strong robustness, no singularity, less chattering, and fast finite-time convergence due to the combined NFTSMC and HOSM control, and requires no prior knowledge of the fault due to TDE-based fault estimation. Finally, simulation results are obtained to verify the effectiveness of the proposed strategy.

  17. Failure-tolerant control for small agile satellites using single-gimbal control moment gyros and magnetic torquers

    Institute of Scientific and Technical Information of China (English)

    Tao Meng; Saburo Matunaga

    2012-01-01

    This paper focuses on the attitude control problem of small agile satellites using single-gimbal control moment gyros (CMG) and magnetic torquers (MTQ).CMGs are regarded as effective torque generators for agile satellites because of their torque amplification capability.However,they are vulnerable to failure due to their complex inner mechanism.In this paper,different failure cases of CMGs are analyzed.A flexible failure-tolerant control strategy is developed by automatically redistributing the required control torque among the operating CMGs and MTQs,with a variable limiter to accommodate the actuator dynamics changes introduced by CMG failures.The performances of maneuvers about different directions under different failure cases are also discussed and examined.Numerical simulations demonstrate that the proposed strategy maintains certain agility in the cases of one or two CMGs failing.Moreover,a survival strategy with only one CMG left is also verified.Both sun-pointing stabilization and earth-pointing stabilization can be achieved in this case,which fulfill some basic mission requirements.

  18. A regulatory framework for shoot stem cell control integrating metabolic, transcriptional, and phytohormone signals.

    Science.gov (United States)

    Schuster, Christoph; Gaillochet, Christophe; Medzihradszky, Anna; Busch, Wolfgang; Daum, Gabor; Krebs, Melanie; Kehle, Andreas; Lohmann, Jan U

    2014-02-24

    Plants continuously maintain pluripotent stem cells embedded in specialized tissues called meristems, which drive long-term growth and organogenesis. Stem cell fate in the shoot apical meristem (SAM) is controlled by the homeodomain transcription factor WUSCHEL (WUS) expressed in the niche adjacent to the stem cells. Here, we demonstrate that the bHLH transcription factor HECATE1 (HEC1) is a target of WUS and that it contributes to SAM function by promoting stem cell proliferation, while antagonizing niche cell activity. HEC1 represses the stem cell regulators WUS and CLAVATA3 (CLV3) and, like WUS, controls genes with functions in metabolism and hormone signaling. Among the targets shared by HEC1 and WUS are phytohormone response regulators, which we show to act as mobile signals in a universal feedback system. Thus, our work sheds light on the mechanisms guiding meristem function and suggests that the underlying regulatory system is far more complex than previously anticipated. Copyright © 2014 Elsevier Inc. All rights reserved.

  19. A BMP regulatory network controls ectodermal cell fate decisions at the neural plate border.

    Science.gov (United States)

    Reichert, Sabine; Randall, Rebecca A; Hill, Caroline S

    2013-11-01

    During ectodermal patterning the neural crest and preplacodal ectoderm are specified in adjacent domains at the neural plate border. BMP signalling is required for specification of both tissues, but how it is spatially and temporally regulated to achieve this is not understood. Here, using a transgenic zebrafish BMP reporter line in conjunction with double-fluorescent in situ hybridisation, we show that, at the beginning of neurulation, the ventral-to-dorsal gradient of BMP activity evolves into two distinct domains at the neural plate border: one coinciding with the neural crest and the other abutting the epidermis. In between is a region devoid of BMP activity, which is specified as the preplacodal ectoderm. We identify the ligands required for these domains of BMP activity. We show that the BMP-interacting protein Crossveinless 2 is expressed in the BMP activity domains and is under the control of BMP signalling. We establish that Crossveinless 2 functions at this time in a positive-feedback loop to locally enhance BMP activity, and show that it is required for neural crest fate. We further demonstrate that the Distal-less transcription factors Dlx3b and Dlx4b, which are expressed in the preplacodal ectoderm, are required for the expression of a cell-autonomous BMP inhibitor, Bambi-b, which can explain the specific absence of BMP activity in the preplacodal ectoderm. Taken together, our data define a BMP regulatory network that controls cell fate decisions at the neural plate border.

  20. A quality control method enhancement concept-Continual improvement of regulatory approved QC methods.

    Science.gov (United States)

    Åsberg, Dennis; Nilsson, Mikael; Olsson, Susanne; Samuelsson, Jörgen; Svensson, Olof; Klick, Silke; Ennis, Julie; Butterworth, Paul; Watt, Denise; Iliadou, Stavroula; Karlsson, Angelica; Walker, Joanne T; Arnot, Kate; Ealer, Norb; Hernqvist, Kerstin; Svensson, Karin; Grinell, Ali; Quist, Per-Ola; Karlsson, Anders; Fornstedt, Torgny

    2016-09-10

    Quality Control methods (QC-methods) play an important role in the overall control strategy for drug manufacturing. However, efficient life-cycle management and continual improvement are hindered due to a variety of post-approval variation legislations across territories and a lack of harmonization of the requirements. As a result, many QC-methods fall behind the technical development. Developing the QC-method in accordance with the Quality by Design guidelines gives the possibility to do continual improvements inside the original Method Operable Design Region (MODR). However, often it is necessary to do changes outside the MODR, e.g. to incorporate new technology that was not available at the time the original method was development. Here, we present a method enhancement concept which allows minor adjustments, within the same measuring principle, outside the original MODR without interaction with regulatory agencies. The feasibility of the concept is illustrated by a case study of a QC-method based on HPLC, assumed to be developed before the introduction of UHPLC, where the switch from HPLC to UHPLC is necessary as a continual improvement strategy. The concept relies on the assumption that the System Suitability Test (SST) and failure modes are relevant for other conditions outside the MODR as well when the same measuring principle is used. It follows that it should be possible to move outside the MODR as long as the SST has passed. All minor modifications of the original, approved QC-method must be re-validated according to a template given in the original submission and a statistical equivalence should be shown between the original and modified QC-methods. To summarize, revalidation is handled within the pharmaceutical quality control system according to internal change control procedures, but without interaction with regulating agencies.

  1. Conserved Cis-Regulatory Modules Control Robustness in Msx1 Expression at Single-Cell Resolution

    Science.gov (United States)

    Vance, Keith W.; Woodcock, Dan J.; Reid, John E.; Bretschneider, Till; Ott, Sascha; Koentges, Georgy

    2015-01-01

    The process of transcription is highly stochastic leading to cell-to-cell variations and noise in gene expression levels. However, key essential genes have to be precisely expressed at the correct amount and time to ensure proper cellular development and function. Studies in yeast and bacterial systems have shown that gene expression noise decreases as mean expression levels increase, a relationship that is controlled by promoter DNA sequence. However, the function of distal cis-regulatory modules (CRMs), an evolutionary novelty of metazoans, in controlling transcriptional robustness and variability is poorly understood. In this study, we used live cell imaging of transfected reporters combined with a mathematical modelling and statistical inference scheme to quantify the function of conserved Msx1 CRMs and promoters in modulating single-cell real-time transcription rates in C2C12 mouse myoblasts. The results show that the mean expression–noise relationship is solely promoter controlled for this key pluripotency regulator. In addition, we demonstrate that CRMs modulate single-cell basal promoter rate distributions in a graded manner across a population of cells. This extends the rheostatic model of CRM action to provide a more detailed understanding of CRM function at single-cell resolution. We also identify a novel CRM transcriptional filter function that acts to reduce intracellular variability in transcription rates and show that this can be phylogenetically separable from rate modulating CRM activities. These results are important for understanding how the expression of key vertebrate developmental transcription factors is precisely controlled both within and between individual cells. PMID:26342140

  2. Small regulatory RNAs control the multi-cellular adhesive lifestyle of Escherichia coli

    DEFF Research Database (Denmark)

    Jørgensen, Mikkel Girke; Nielsen, Jesper Sejrup; Boysen, Anders

    2012-01-01

    Small regulatory RNA molecules have recently been recognized as important regulatory elements of developmental processes in both eukaryotes and bacteria. We here describe a striking example in Escherichia coli that can switch between a single-cell motile lifestyle and a multi-cellular, sessile...

  3. Mobile medical and health apps: state of the art, concerns, regulatory control and certification.

    Science.gov (United States)

    Boulos, Maged N Kamel; Brewer, Ann C; Karimkhani, Chante; Buller, David B; Dellavalle, Robert P

    2014-01-01

    This paper examines the state of the art in mobile clinical and health-related apps. A 2012 estimate puts the number of health-related apps at no fewer than 40,000, as healthcare professionals and consumers continue to express concerns about the quality of many apps, calling for some form of app regulatory control or certification to be put in place. We describe the range of apps on offer as of 2013, and then present a brief survey of evaluation studies of medical and health-related apps that have been conducted to date, covering a range of clinical disciplines and topics. Our survey includes studies that highlighted risks, negative issues and worrying deficiencies in existing apps. We discuss the concept of 'apps as a medical device' and the relevant regulatory controls that apply in USA and Europe, offering examples of apps that have been formally approved using these mechanisms. We describe the online Health Apps Library run by the National Health Service in England and the calls for a vetted medical and health app store. We discuss the ingredients for successful apps beyond the rather narrow definition of 'apps as a medical device'. These ingredients cover app content quality, usability, the need to match apps to consumers' general and health literacy levels, device connectivity standards (for apps that connect to glucometers, blood pressure monitors, etc.), as well as app security and user privacy. 'Happtique Health App Certification Program' (HACP), a voluntary app certification scheme, successfully captures most of these desiderata, but is solely focused on apps targeting the US market. HACP, while very welcome, is in ways reminiscent of the early days of the Web, when many "similar" quality benchmarking tools and codes of conduct for information publishers were proposed to appraise and rate online medical and health information. It is probably impossible to rate and police every app on offer today, much like in those early days of the Web, when people

  4. A Direct Design from Input/Output Data of Fault-Tolerant Control System Based on GIMC Structure

    Science.gov (United States)

    Sakuishi, Tsubasa; Yubai, Kazuhiro; Hirai, Junji

    This paper deals with a design method of fault-tolerant control system based on Generalized Internal Model Control (GIMC) structure consisting of a standard outer loop feedback controller and an extra inner loop controller. The distinguished feature of GIMC structure is that the controller design for performance and robustness may be done separately. The outer loop controller is designed for nominal performance using some controller synthesis to meet (nominal) control specification, while the inner loop controller is designed to make a trade-off between robustness and performance. This feature is suitable for fault-tolerant control. The outer loop controller is designed for fault-free case, and the inner loop controller for faulty case. In the conventional methods, the inner loop controller is designed to maximize the robust stability margin without information on fault. Therefore, the performance in the faulty case tends to become conservative. In this paper, the inner loop controller is directly designed from experimental data collected from the faulty system. Since the collected data contains information on the fault, conservativeness in the conventional methods is decreased. The inner loop controller is designed by Virtual Reference Feedback Tuning (VRFT). VRFT is a direct design method from input-output data without identifying any models. Since complexity of the controller can be specified by the designer, no complexity reduction has to be required, which becomes advantageous upon implementation. The effectiveness of the proposed design method is confirmed by an experiment.

  5. Fitotoxicidade do alumínio: efeitos, mecanismo de tolerância e seu controle genético

    Directory of Open Access Journals (Sweden)

    Echart Cinara Lima

    2001-01-01

    Full Text Available Solos ácidos associados a metais tóxicos são uma das maiores restrições à produção agrícola e, entre os metais, o alumínio destaca-se como um dos maiores problemas em solos com pH £ 5,0. O melhoramento para tolerância ao alumínio é considerado um excelente caminho para aumentar o desempenho de plantas economicamente importantes cultivadas em solos ácidos ricos nesse metal, como os que ocorrem no sul do Brasil. Para isso, é necessário identificar, entre os diferentes cultivares, aqueles que apresentam mecanismos de tolerância, compreender como esses cultivares agem em sua presença, bem como conhecer o controle genético da tolerância ao alumínio. Este trabalho apresenta uma revisão sobre o assunto, abordando as formas de alumínio presentes no solo, seu efeito sobre as plantas, os mecanismos de tolerância e o controle genético desta tolerância em diferentes grupos de cereais.

  6. Efficacy and tolerability of biologic and nonbiologic systemic treatments for moderate-to-severe psoriasis: meta-analysis of randomized controlled trials.

    Science.gov (United States)

    Schmitt, J; Zhang, Z; Wozel, G; Meurer, M; Kirch, W

    2008-09-01

    The comparative efficacy and tolerability of conventional and biologic treatments for moderate-to-severe plaque psoriasis are unknown. To perform a systematic review and meta-analysis of randomized controlled trials (RCTs) reporting efficacy of systemic treatments approved for moderate-to-severe psoriasis by means of the Psoriasis Area and Severity Index (PASI). We identified relevant articles by systematic electronic searches (Cochrane Library, Medline, Embase, Scopus). Efficacy was defined as proportion of participants with >or= 75% decrease in PASI (PASI-75) at primary efficacy measurement (week 8-16). PASI-75 response rates of double-blind placebo-controlled trials were summarized as risk differences (RDs) and pooled using random effects models. Tolerability was assessed from rates of withdrawals and adverse events. Twenty-four RCTs totalling 9384 patients were analysed qualitatively. Sixteen double-blind placebo-controlled trials were eligible for meta-analysis. Infliximab was significantly superior to all other interventions [RD 77%, 95% confidence interval (CI) 72-81%]. Adalimumab (RD 64%, 95% CI 61-68%) was superior to ciclosporin (RD 33%, 95% CI 13-52%), efalizumab (RD 24%, 95% CI 19-30%), etanercept 50 mg twice weekly (RD 44%, 95% CI 40-48%) and etanercept 25 mg twice weekly (RD 30%, 95% CI 25-35%). Efalizumab was significantly less efficacious than fumaric acid esters (RD 48%, 95% CI 32-64%). Rates of withdrawals due to adverse events were highest for methotrexate and fumaric acid esters. The efficacy of systemic agents approved for moderate-to-severe psoriasis differs considerably both within and between biologics and nonbiologics. Infliximab is most efficacious, followed by adalimumab. Patients receiving infliximab have an excess chance of 77% over placebo to achieve PASI-75 response. Published evidence questions regulatory guidelines that recommend biologics as second-line therapy for moderate-to-severe plaque psoriasis.

  7. New regulatory circuit controlling spatial and temporal gene expression in the sea urchin embryo oral ectoderm GRN.

    Science.gov (United States)

    Li, Enhu; Materna, Stefan C; Davidson, Eric H

    2013-10-01

    The sea urchin oral ectoderm gene regulatory network (GRN) model has increased in complexity as additional genes are added to it, revealing its multiple spatial regulatory state domains. The formation of the oral ectoderm begins with an oral-aboral redox gradient, which is interpreted by the cis-regulatory system of the nodal gene to cause its expression on the oral side of the embryo. Nodal signaling drives cohorts of regulatory genes within the oral ectoderm and its derived subdomains. Activation of these genes occurs sequentially, spanning the entire blastula stage. During this process the stomodeal subdomain emerges inside of the oral ectoderm, and bilateral subdomains defining the lateral portions of the future ciliary band emerge adjacent to the central oral ectoderm. Here we examine two regulatory genes encoding repressors, sip1 and ets4, which selectively prevent transcription of oral ectoderm genes until their expression is cleared from the oral ectoderm as an indirect consequence of Nodal signaling. We show that the timing of transcriptional de-repression of sip1 and ets4 targets which occurs upon their clearance explains the dynamics of oral ectoderm gene expression. In addition two other repressors, the direct Nodal target not, and the feed forward Nodal target goosecoid, repress expression of regulatory genes in the central animal oral ectoderm thereby confining their expression to the lateral domains of the animal ectoderm. These results have permitted construction of an enhanced animal ectoderm GRN model highlighting the repressive interactions providing precise temporal and spatial control of regulatory gene expression. © 2013 Elsevier Inc. All rights reserved.

  8. Filtering and fault tolerant control of parameter-varying time-delay systems and applications

    Science.gov (United States)

    Mohammadpour Velni, Javad

    This dissertation addresses some open problems in control systems theory. The problems considered include the dynamic controller and filter design for Linear Parameter Varying (LPV) time-delay systems, the reconfigurable control design in Fault Tolerant Control Systems (FTCS) and fault diagnostics in Diesel engines. In the first part of this thesis, we investigate the problem of designing parameter-dependent filters for output estimation of LPV time-delay systems. The filters are designed such that the filtering error system guarantees an optimum level of H2 or Hinfinity performance. A state-delay term is included in the filter dynamics to reduce the design conservatism and improve the performance. The Linear Matrix Inequality (LMI)-based synthesis conditions developed for the filter design purposes are categorized into the rate-dependent and delay-dependent conditions which could handle the time-varying state-delay and bounded small delay cases, respectively. Among these two, the latter one is shown to provide a significant reduction in the conservativeness in the filter design. The second part of the thesis examines the analysis and synthesis of Fault Tolerant Control (FTC) systems in an LPV framework. For reconfigurable control design purposes, the information from Fault Detection and Isolation (FDI) module, that provides an estimate of the fault parameters, is utilized to schedule the controller matrices. We will also present a formulation that incorporates the factor of detection delay in the FTC supervisory system. It is shown that including this delay in the synthesis conditions leads to improved performance and reduced control effort. For analysis of the FTC systems including time-delay, where the fault parameters might be identified inaccurately, we first introduce the notion of brief instability for LPV time-delay systems. In these systems it is possible that the output trajectory converges to zero even though there are parameter trajectories for which

  9. Long-term tolerability of inhaled human insulin (Exubera) in patients with poorly controlled type 2 diabetes

    DEFF Research Database (Denmark)

    Barnett, A H; Lange, P; Dreyer, M;

    2007-01-01

    OBJECTIVE: Inhaled human insulin (Exubera; EXU) has shown encouraging tolerability in short-term trials. We evaluated the safety profile of EXU after long-term exposure. DESIGN: In two, open-label, 2-year studies patients poorly controlled on a sulphonylurea were randomised to adjunctive EXU or m...

  10. cis-Regulatory control of the initial neurogenic pattern of onecut gene expression in the sea urchin embryo.

    Science.gov (United States)

    Barsi, Julius C; Davidson, Eric H

    2016-01-01

    Specification of the ciliated band (CB) of echinoid embryos executes three spatial functions essential for postgastrular organization. These are establishment of a band about 5 cells wide which delimits and bounds other embryonic territories; definition of a neurogenic domain within this band; and generation within it of arrays of ciliary cells that bear the special long cilia from which the structure derives its name. In Strongylocentrotus purpuratus the spatial coordinates of the future ciliated band are initially and exactly determined by the disposition of a ring of cells that transcriptionally activate the onecut homeodomain regulatory gene, beginning in blastula stage, long before the appearance of the CB per se. Thus the cis-regulatory apparatus that governs onecut expression in the blastula directly reveals the genomic sequence code by which these aspects of the spatial organization of the embryo are initially determined. We screened the entire onecut locus and its flanking region for transcriptionally active cis-regulatory elements, and by means of BAC recombineered deletions identified three separated and required cis-regulatory modules that execute different functions. The operating logic of the crucial spatial control module accounting for the spectacularly precise and beautiful early onecut expression domain depends on spatial repression. Previously predicted oral ectoderm and aboral ectoderm repressors were identified by cis-regulatory mutation as the products of goosecoid and irxa genes respectively, while the pan-ectodermal activator SoxB1 supplies a transcriptional driver function.

  11. Regulatory elements controlling pituitary-specific expression of the human prolactin gene.

    Science.gov (United States)

    Peers, B; Voz, M L; Monget, P; Mathy-Hartert, M; Berwaer, M; Belayew, A; Martial, J A

    1990-09-01

    We have performed transfection and DNase I footprinting experiments to investigate pituitary-specific expression of the human prolactin (hPRL) gene. When fused to the chloramphenicol acetyltransferase (CAT) reporter gene, 5,000 base pairs of the 5'-flanking sequences of the hPRL gene were able to drive high cat gene expression in prolactin-expressing GH3B6 cells specifically. Deletion analysis indicated that this pituitary-specific expression was controlled by three main positive regulatory regions. The first was located just upstream from the TATA box between coordinates -40 and -250 (proximal region). We have previously shown that three motifs of this region bind the pituitary-specific Pit-1 factor. The second positive region was located in the vicinity of coordinates -1300 to -1750 (distal region). DNase I footprinting assays revealed that eight DNA motifs of this distal region bound protein Pit-1 and that two other motifs were recognized by ubiquitous factors, one of which seems to belong to the AP-1 (jun) family. The third positive region was located further upstream, between -3500 and -5000 (superdistal region). This region appears to enhance transcription only in the presence of the distal region.

  12. The HY5-PIF regulatory module coordinates light and temperature control of photosynthetic gene transcription.

    Directory of Open Access Journals (Sweden)

    Gabriela Toledo-Ortiz

    2014-06-01

    Full Text Available The ability to interpret daily and seasonal alterations in light and temperature signals is essential for plant survival. This is particularly important during seedling establishment when the phytochrome photoreceptors activate photosynthetic pigment production for photoautotrophic growth. Phytochromes accomplish this partly through the suppression of phytochrome interacting factors (PIFs, negative regulators of chlorophyll and carotenoid biosynthesis. While the bZIP transcription factor long hypocotyl 5 (HY5, a potent PIF antagonist, promotes photosynthetic pigment accumulation in response to light. Here we demonstrate that by directly targeting a common promoter cis-element (G-box, HY5 and PIFs form a dynamic activation-suppression transcriptional module responsive to light and temperature cues. This antagonistic regulatory module provides a simple, direct mechanism through which environmental change can redirect transcriptional control of genes required for photosynthesis and photoprotection. In the regulation of photopigment biosynthesis genes, HY5 and PIFs do not operate alone, but with the circadian clock. However, sudden changes in light or temperature conditions can trigger changes in HY5 and PIFs abundance that adjust the expression of common target genes to optimise photosynthetic performance and growth.

  13. Comparing regulatory regimens for pesticide control in 22 countries: toward a new generation of pesticide regulation.

    Science.gov (United States)

    Schmidt-Bleek, F; Marchal, M M

    1993-06-01

    Some historical developments of chemical control legislation are being traced, from specialized laws, such as pesticide acts, to the 1970/1980 generation of generalized legal instruments involving all commercial chemicals. It is then argued that from an environmental protection point of view, a "cradle-to-grave" assessment will henceforth be necessary. Ecological guidelines and indicators for the assessment of the life-cyclewide environmental impact intensity of economic outputs are presented. The international implications of the worldwide trade in pesticides are being considered. As the use of pesticides has invariably transfrontier effects and since unilateral import restrictions due to environmental quality requirements constitute nontariff barriers to trade, a high degree of international harmonization is desirable. This point is also of some importance with a view to the GATT negotiations and the chances for exporting chemicals from developing and postsocialist countries into industrialized hard currency areas in the future. The results of a comprehensive review in 1990/1991 of the regulatory pesticide management regime in 22 countries are presented, comprising countries from Central and Eastern Europe as well as the industrialized Western countries. The review is based upon the returns to a questionnaire which had been designed jointly by the International Institute of Applied Systems Analysis (IIASA) and the Regional Office of the WHO in Copenhagen. The information received was subsequently validated by the competent authorities. Taking all observations into consideration, the framework of an advanced prototype legislative framework for pesticides management is proposed.

  14. Redox regulation and overreduction control in the photosynthesizing cell: complexity in redox regulatory networks.

    Science.gov (United States)

    Oelze, Marie-Luise; Kandlbinder, Andrea; Dietz, Karl-Josef

    2008-11-01

    Regulation of the photosynthetic apparatus between efficient energy conversion at low light and avoidance of overreduction and damage development at excess light resembles dangerous navigating between Scylla and Charybdis. Photosynthesis is a high rate redox metabolic pathway that generates redox intermediates with extreme redox potentials and eventually reactive oxygen species and oxidative stress. Therefore it is not surprising that the states of defined redox reactions in the chloroplast provide the predominant information and thus directly or indirectly the decisive signals for the multilevel control of cell activities in the chloroplast, cytoplasm, mitochondrion and nucleus. This review elaborates on the diversity of photosynthesis-derived redox signals such as the plastoquinone and thiol redox state that regulate and coordinate light use efficiency, electron transport activity, metabolic reactions, gene transcription and translation not only in the chloroplast but through retrograde signaling also essentially in all other cell compartments. The synergistic and antagonistic interrelations between the redox-dependent signaling pathways and their interactions with other signals such as abscisic acid and tetrapyrol intermediates constitute a redundant and probably buffered regulatory network to optimize performance of photosynthesis on the cellular and whole leaf level.

  15. Self-sustained motion of microcapsules on a substrate controlled via the repressilator regulatory network

    Science.gov (United States)

    Shum, Henry; Yashin, Victor; Balazs, Anna

    2014-11-01

    We design microcapsules that undergo self-induced motion in a fluid along a substrate and are able to collectively self-organize when controlled by a biomimetic signaling network. Three microcapsules act as localized sources of distinct chemicals that diffuse through the fluid. The production rate of each chemical is modulated by a regulatory network known as the repressilator: each species represses the production of the next in a cycle. We show that this system can exhibit sustained oscillations. We then allow the diffusing species to adsorb onto the substrate, altering the surface interaction energy. Gradients in surface energy lead to motion of the microcapsules. We find that regulation via the repressilator gives rise to qualitatively different outcomes. Chemical oscillations can facilitate aggregation of the microcapsules and the aggregate can undergo sustained translational or oscillatory motion. Numerical simulation of the fluid flow, microcapsule dynamics and concentration fields is achieved by a combination of the lattice Boltzmann, immersed boundary and finite difference methods. We assess the role of hydrodynamic interactions by comparison with a simplified model that assumes a constant drag coefficient relating the force on a microcapsule to its velocity.

  16. Aflatoxin control--how a regulatory agency managed risk from an unavoidable natural toxicant in food and feed.

    Science.gov (United States)

    Park, D L; Stoloff, L

    1989-04-01

    The control by the Food and Drug Administration (FDA) of aflatoxin, a relatively recently discovered, unavoidable natural contaminant produced by specific molds that invade a number of basic food and feedstuffs, provides an example of the varying forces that affect risk assessment and management by a regulatory Agency. This is the story of how the FDA responded to the initial discovery of a potential carcinogenic hazard to humans in a domestic commodity, to the developing information concerning the nature of the hazard, to the economic and political pressures that are created by the impact of natural forces on regulatory controls, and to the restraints of laws within which the Agency must work. This story covers four periods: the years of discovery and action decisions on the basis of meager knowledge and the fear of cancer; the years of tinkering on paper with the regulatory process, the years of digestion of the accumulating knowledge, and the application of that knowledge to actions forced by natural events; and an audit of the current status of knowledge about the hazard from aflatoxin, and proposals for regulatory control based on that knowledge.

  17. The efficacy and tolerability of 'polypills': meta-analysis of randomised controlled trials.

    Directory of Open Access Journals (Sweden)

    C Raina Elley

    Full Text Available BACKGROUND: To assess the blood pressure and lipid-lowering efficacy and tolerability of 'polypills' used in cardiovascular disease prevention trials. METHODOLOGY/PRINCIPAL FINDINGS: Systematic review and meta-analysis. SEARCH STRATEGY: The Cochrane Central Register of Controlled Trials, Medline, and PubMed databases were searched for eligible trials. Study inclusion criteria: Randomised controlled trials of at least six weeks duration, which compared a 'polypill' (that included at least one anti-hypertensive and one lipid-lowering medication with a placebo (or one active component. OUTCOME MEASURES: Change from baseline in systolic and diastolic blood pressures, and total and LDL-cholesterol; discontinuation of study medication and reported adverse effects. Of 44 potentially eligible studies, six trials (including 2,218 patients without previous cardiovascular disease fulfilled the inclusion criteria. Compared with placebo, 'polypills' reduced systolic blood pressure by -9.2 mmHg (95% confidence interval (CI: -13.4, -5.0 diastolic blood pressure by -5.0 mmHg (95%CI: -7.4, -2.6, total cholesterol by -1.22 mmol/L (95%CI: -1.60, -0.84 and LDL-cholesterol by -1.02 mmol/L (95%CI: -1.37, -0.67. However, those taking a 'polypill' (vs. placebo or component were more likely to discontinue medication (20% vs 14% (Odds ratio: 1.5 (95% CI: 1.2, 1.9. There was no significant difference in reported adverse effects amongst those on a 'polypill' (36% vs. 28% (OR: 1.3 (95%CI: 0.7, 2.5. There was high statistical heterogeneity in comparisons for blood pressure and lipid-lowering but use of random-effects and quality-effects models produced very similar results. CONCLUSIONS/SIGNIFICANCE: Compared with placebo, the 'polypills' reduced blood pressure and lipids. Tolerability was lower amongst those on 'polypills' than those on placebo or one component, but differences were moderate. Effectiveness trials are needed to help clarify the status of 'polypills' in

  18. Control of Her-2 tumor immunity and thyroid autoimmunity by MHC and regulatory T cells.

    Science.gov (United States)

    Jacob, Jennifer B; Kong, Yi-chi M; Meroueh, Chady; Snower, Daniel P; David, Chella S; Ho, Ye-Shih; Wei, Wei-Zen

    2007-07-15

    Immune reactivity to self-antigens in both cancer and autoimmune diseases can be enhanced by systemic immune modulation, posing a challenge in cancer immunotherapy. To distinguish the genetic and immune regulation of tumor immunity versus autoimmunity, immune responses to human ErbB-2 (Her-2) and mouse thyroglobulin (mTg) were tested in transgenic mice expressing Her-2 that is overexpressed in several cancers, and HLA-DRB1*0301 (DR3) that is associated with susceptibility to several human autoimmune diseases, as well as experimental autoimmune thyroiditis (EAT). To induce Her-2 response, mice were electrovaccinated with pE2TM and pGM-CSF encoding the extracellular and transmembrane domains of Her-2 and the murine granulocyte macrophage colony-stimulating factor, respectively. To induce EAT, mice received mTg i.v. with or without lipopolysaccharide. Depletion of regulatory T cells (Treg) with anti-CD25 monoclonal antibody enhanced immune reactivity to Her-2 as well as mTg, showing control of both Her-2 and mTg responses by Treg. When immunized with, Her-2xDR3 and B6xDR3 mice expressing H2(b)xDR3 haplotype developed more profound mTg response and thyroid pathology than Her-2 or B6 mice that expressed the EAT-resistant H2(b) haplotype. In Her-2xDR3 mice, the response to mTg was further amplified when mice were also immunized with pE2TM and pGM-CSF. On the contrary, Her-2 reactivity was comparable whether mice expressed DR3 or not. Therefore, induction of Her-2 immunity was independent of DR3 but development of EAT was dictated by this allele, whereas Tregs control the responses to both self-antigens. These results warrant close monitoring of autoimmunity during cancer immunotherapy, particularly in patients with susceptible MHC class II alleles.

  19. CTLA-4 (CD152) controls homeostasis and suppressive capacity of regulatory T cells in mice.

    Science.gov (United States)

    Kolar, Paula; Knieke, Karin; Hegel, J Kolja E; Quandt, Dagmar; Burmester, Gerd-R; Hoff, Holger; Brunner-Weinzierl, Monika C

    2009-01-01

    CD4+CD25+ regulatory T cells (known as Treg cells) suppress unwanted and autoreactive T cell responses. Treg cells express the costimulatory molecule CTLA-4 intracellularly, but the mechanisms by which Treg cells exploit CTLA-4 signaling remain unclear. The present study was undertaken to investigate the role of CTLA-4 in controlling the homeostasis and suppressive function of Treg cells. Murine Treg cells were analyzed by flow cytometry for coexpression of CTLA-4 and typical Treg cell-expressed molecules, and the influence of CTLA-4 on T cell proliferation, suppression, and apoptosis was investigated by in vitro assays. To analyze the importance of CTLA-4 in Treg cell-mediated suppression in vivo, wild-type Treg cells were transferred into CTLA-4-deficient mice displaying lymphoproliferation, and survival was monitored over time. A strong correlation between expression of forkhead box P3 and ex vivo expression of CTLA-4 in Treg cells was observed. Inhibition of CTLA-4 signaling in Treg cells during in vitro stimulation increased cell cycling and led to enhanced activation-induced cell death (AICD), which was mediated by CD95/CD95 ligand-induced activation of caspases. Blockade of CTLA-4 signaling resulted in impairment of the suppressive capacity of Treg cells. Despite these effects, high amounts of Treg cells persisted in CTLA-4-deficient mice. Results of transfer experiments in CTLA-4-deficient mice showed that the mice had a significantly prolonged lifespan when CTLA-4-competent Treg cells were injected. Expression of CTLA-4 on Treg cells serves to control T cell proliferation, to confer resistance against AICD, and to maintain the suppressive function of Treg cells.

  20. Fault Diagnosis and Fault-Tolerant Control of Uncertain Robot Manipulators Using High-Order Sliding Mode

    Directory of Open Access Journals (Sweden)

    Mien Van

    2016-01-01

    Full Text Available A robust fault diagnosis and fault-tolerant control (FTC system for uncertain robot manipulators without joint velocity measurement is presented. The actuator faults and robot manipulator component faults are considered. The proposed scheme is designed via an active fault-tolerant control strategy by combining a fault diagnosis scheme based on a super-twisting third-order sliding mode (STW-TOSM observer with a robust super-twisting second-order sliding mode (STW-SOSM controller. Compared to the existing FTC methods, the proposed FTC method can accommodate not only faults but also uncertainties, and it does not require a velocity measurement. In addition, because the proposed scheme is designed based on the high-order sliding mode (HOSM observer/controller strategy, it exhibits fast convergence, high accuracy, and less chattering. Finally, computer simulation results for a PUMA560 robot are obtained to verify the effectiveness of the proposed strategy.

  1. A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control.

    Science.gov (United States)

    Huang, Xin-Yuan; Chao, Dai-Yin; Gao, Ji-Ping; Zhu, Mei-Zhen; Shi, Min; Lin, Hong-Xuan

    2009-08-01

    Abiotic stresses, such as drought and salinity, lead to crop growth damage and a decrease in crop yields. Stomata control CO(2) uptake and optimize water use efficiency, thereby playing crucial roles in abiotic stress tolerance. Hydrogen peroxide (H(2)O(2)) is an important signal molecule that induces stomatal closure. However, the molecular pathway that regulates the H(2)O(2) level in guard cells remains largely unknown. Here, we clone and characterize DST (drought and salt tolerance)-a previously unknown zinc finger transcription factor that negatively regulates stomatal closure by direct modulation of genes related to H(2)O(2) homeostasis-and identify a novel pathway for the signal transduction of DST-mediated H(2)O(2)-induced stomatal closure. Loss of DST function increases stomatal closure and reduces stomatal density, consequently resulting in enhanced drought and salt tolerance in rice. These findings provide an interesting insight into the mechanism of stomata-regulated abiotic stress tolerance, and an important genetic engineering approach for improving abiotic stress tolerance in crops.

  2. PROTECTING ECOSYSTEMS BY WAY OF BIOLOGICAL CONTROL: CURSORY REFLECTIONS ON THE MAIN REGULATORY INSTRUMENTS FOR BIOLOGICAL CONTROL AGENTS, PRESENT AND FUTURE

    Directory of Open Access Journals (Sweden)

    R Alberts

    2013-06-01

    Full Text Available Although there are numerous threats to ecosystems and the resultant ecosystem services, alien and invasive plants (AIP have been identified as being one of the major causes of ecosystem destruction. In addressing the threat of alien and invasive plants through the use of various mechanisms, the regulatory framework imposed by legislation is key in ensuring that that controlling AIPs does in fact not do more harm than good. One such control mechanism, which has the potential to do wonders or wreak havoc if not adroitly implemented, is that of using biological control agents. This contribution provides a brief overview on the three main regulatory instruments used to control biological control agents in South Africa, namely the Conservation of Agricultural Resources Act 43 of 1983, the Agricultural Pests Act 36 of 1983 and the National Environmental Management: Biodiversity Act 10 of 2004. It also considers possible future developments on the regulation of biological control agents.

  3. DSP-Based Sensor Fault Detection and Post Fault-Tolerant Control of an Induction Motor-Based Electric Vehicle

    Directory of Open Access Journals (Sweden)

    Bekheïra Tabbache

    2012-01-01

    Full Text Available This paper deals with sensor fault detection within a reconfigurable direct torque control of an induction motor-based electric vehicle. The proposed strategy concerns current, voltage, and speed sensors faults that are detected and followed by post fault-tolerant control to allow the vehicle continuous operation. The proposed approach is validated through experiments on an induction motor drive and simulations on an electric vehicle using a European urban and extraurban driving cycle.

  4. Robust Fault-Tolerant Control for Satellite Attitude Stabilization Based on Active Disturbance Rejection Approach with Artificial Bee Colony Algorithm

    OpenAIRE

    Fei Song; Shiyin Qin

    2014-01-01

    This paper proposed a robust fault-tolerant control algorithm for satellite stabilization based on active disturbance rejection approach with artificial bee colony algorithm. The actuating mechanism of attitude control system consists of three working reaction flywheels and one spare reaction flywheel. The speed measurement of reaction flywheel is adopted for fault detection. If any reaction flywheel fault is detected, the corresponding fault flywheel is isolated and the spare reaction flywhe...

  5. Kaempferol Promotes Transplant Tolerance by Sustaining CD4+FoxP3+ Regulatory T Cells in the Presence of Calcineurin Inhibitor.

    Science.gov (United States)

    Zeng, Y Q; Liu, X S; Wu, S; Zou, C; Xie, Q; Xu, S M; Jin, X W; Li, W; Zhou, A; Dai, Z

    2015-07-01

    Calcineurin inhibitor cyclosporine is widely used as an immunosuppressant in clinic. However, mounting evidence has shown that cyclosporine hinders tolerance induction by dampening Tregs. Therefore, it is of paramount importance to overcome this pitfall. Kaempferol was reported to inhibit DC function. Here, we found that kaempferol delayed islet allograft rejection. Combination of kaempferol and low-dose, but not high-dose, of cyclosporine induced allograft tolerance in majority of recipient mice. Although kaempferol plus either dose of cyclosporine largely abrogated proliferation of graft-infiltrating T cells and their CTL activity, both proliferation and CTL activity in mice treated with kaempferol plus low-dose, but not high-dose, cyclosporine reemerged rapidly upon treatment withdrawal. Kaempferol increased CD4+FoxP3+ Tregs both in transplanted mice and in vitro, likely by suppressing DC maturation and their IL-6 expression. Reduction in Tregs by low dose of cyclosporine was reversed by kaempferol. Kaempferol-induced Tregs exhibited both allospecific and non-allospecific suppression. Administering IL-6 abrogated allograft tolerance induced by kaempferol and cyclosporine via diminishing CD4+FoxP3+ Tregs. Thus, for the first time, we demonstrated that kaempferol promotes transplant tolerance in the presence of low dose of cyclosporine, which allows for sufficient Treg generation while minimizing side effects, resulting in much-needed synergy between kaempferol and cyclosporine.

  6. Increasing the dynamic control space of mammalian transcription devices by combinatorial assembly of homologous regulatory elements from different bacterial species.

    Science.gov (United States)

    Bacchus, William; Weber, Wilfried; Fussenegger, Martin

    2013-01-01

    Prokaryotic transcriptional regulatory elements are widely utilized building blocks for constructing regulatory genetic circuits adapted for mammalian cells and have found their way into a broad range of biotechnological applications. Prokaryotic transcriptional repressors, fused to eukaryotic transactivation or repression domains, compose the transcription factor, which binds and adjusts transcription from chimeric promoters containing the repressor-specific operator sequence. Escherichia coli and Chlamydia trachomatis share common features in the regulatory mechanism of the biosynthesis of l-tryptophan. The repressor protein TrpR of C. trachomatis regulates the trpRBA operon and the TrpR of E. coli regulates the trpEDCBA operon, both requiring l-tryptophan as a co-repressor. Fusion of these bacterial repressors to the VP16 transactivation domain of Herpes simplex virus creates synthetic transactivators that could bind and activate chimeric promoters, assembled by placing repressor-specific operator modules adjacent to a minimal promoter, in an l-tryptophan-adjustable manner. Combinations of different transactivator and promoter variants from the same or different bacterial species resulted in a multitude of regulatory systems where l-tryptophan regulation properties, background noise, and maximal gene expression levels were significantly diverse. Different l-tryptophan analogues showed diverse regulatory capacity depending on the promoter/transactivator combination. We believe the systems approach to rationally choose promoters, transactivators and inducer molecules, to obtain desired and predefined genetic expression dynamics and control profiles, will significantly advance the design of new regulatory circuits as well as improving already existing ones. Copyright © 2012 Elsevier Inc. All rights reserved.

  7. Autophagy-dependent regulatory T cells are critical for the control of graft-versus-host disease

    Science.gov (United States)

    Le Texier, Laëtitia; Lineburg, Katie E.; Leveque-El Mouttie, Lucie; Nicholls, Jemma; Melino, Michelle; Nalkurthi, Blessy C.; Alexander, Kylie A.; Teal, Bianca; Blake, Stephen J.; Souza-Fonseca-Guimaraes, Fernando; Engwerda, Christian R.; Kuns, Rachel D.; Lane, Steven W.; Teh, Charis; Gray, Daniel; Clouston, Andrew D.; Nilsson, Susan K.; Blazar, Bruce R.; Hill, Geoffrey R.; MacDonald, Kelli P.A.

    2016-01-01

    Regulatory T cells (Tregs) play a crucial role in the maintenance of peripheral tolerance. Quantitative and/or qualitative defects in Tregs result in diseases such as autoimmunity, allergy, malignancy, and graft-versus-host disease (GVHD), a serious complication of allogeneic stem cell transplantation (SCT). We recently reported increased expression of autophagy-related genes (Atg) in association with enhanced survival of Tregs after SCT. Autophagy is a self-degradative process for cytosolic components that promotes cell homeostasis and survival. Here, we demonstrate that the disruption of autophagy within FoxP3+ Tregs (B6.Atg7fl/fl-FoxP3cre+) resulted in a profound loss of Tregs, particularly within the bone marrow (BM). This resulted in dysregulated effector T cell activation and expansion, and the development of enterocolitis and scleroderma in aged mice. We show that the BM compartment is highly enriched in TIGIT+ Tregs and that this subset is differentially depleted in the absence of autophagy. Moreover, following allogeneic SCT, recipients of grafts from B6.Atg7fl/fl-FoxP3cre+ donors exhibited reduced Treg reconstitution, exacerbated GVHD, and reduced survival compared with recipients of B6.WT-FoxP3cre+ grafts. Collectively, these data indicate that autophagy-dependent Tregs are critical for the maintenance of tolerance after SCT and that the promotion of autophagy represents an attractive immune-restorative therapeutic strategy after allogeneic SCT. PMID:27699243

  8. A Self-regulatory System of Interlinked Signaling Feedback Loops Controls Mouse Limb Patterning

    Science.gov (United States)

    Benazet, Jean-Denis; Bischofberger, Mirko; Tiecke, Eva; Gonalves, Alexandre; Martin, James F.; Zuniga, Aime; Naef, Felix; Zeller, Rolf

    Developmental pathways need to be robust against environmental and genetic variation to enable reliable morphogenesis. Here, we take a systems biology approach to explain how robustness is achieved in the developing mouse limb, a classical model of organogenesis. By combining quantitative genetics with computational modeling we established a computational model of multiple interlocked feedback modules, involving sonic hedgehog (SHH) morphogen, fibroblast growth factor (FGFs) signaling, bone morphogenetic protein (BMP) and its antagonist GREM1. Earlier modeling work had emphasized the versatile kinetic characteristics of interlocked feedback loops operating at different time scales. Here we develop and then validate a similar computational model to show how BMP4 first initiates and SHH then propagates feedback in the network through differential transcriptional regulation of Grem1 to control digit specification. This switch occurs by linking a fast BMP4/GREM1 module to a slower SHH/GREM1/FGF feedback loop. Simulated gene expression profiles modeled normal limb development as well those of single-gene knockouts. Sensitivity analysis showed how the model was robust and insensitive to variability in parameters. A surprising prediction of the model was that an early Bmp4 signal is essential to kick-start Grem1 expression and the digit specification system. We experimentally validated the prediction using inducible alleles and showed that early, but not late, removal of Bmp4 dramatically disrupted limb development. Sensitivity analysis showed how robustness emerges from this circuitry. This study shows how modeling and computation can help us understand how self-regulatory signaling networks achieve robust regulation of limb development, by exploiting interconnectivity among the three signaling pathways. We expect that similar computational analyses will shed light on the origins of robustness in other developmental systems, and I will discuss some recent examples from

  9. Selective translational control of the Alzheimer amyloid precursor protein transcript by iron regulatory protein-1.

    Science.gov (United States)

    Cho, Hyun-Hee; Cahill, Catherine M; Vanderburg, Charles R; Scherzer, Clemens R; Wang, Bin; Huang, Xudong; Rogers, Jack T

    2010-10-08

    Iron influx increases the translation of the Alzheimer amyloid precursor protein (APP) via an iron-responsive element (IRE) RNA stem loop in its 5'-untranslated region. Equal modulated interaction of the iron regulatory proteins (IRP1 and IRP2) with canonical IREs controls iron-dependent translation of the ferritin subunits. However, our immunoprecipitation RT-PCR and RNA binding experiments demonstrated that IRP1, but not IRP2, selectively bound the APP IRE in human neural cells. This selective IRP1 interaction pattern was evident in human brain and blood tissue from normal and Alzheimer disease patients. We computer-predicted an optimal novel RNA stem loop structure for the human, rhesus monkey, and mouse APP IREs with reference to the canonical ferritin IREs but also the IREs encoded by erythroid heme biosynthetic aminolevulinate synthase and Hif-2α mRNAs, which preferentially bind IRP1. Selective 2'-hydroxyl acylation analyzed by primer extension analysis was consistent with a 13-base single-stranded terminal loop and a conserved GC-rich stem. Biotinylated RNA probes deleted of the conserved CAGA motif in the terminal loop did not bind to IRP1 relative to wild type probes and could no longer base pair to form a predicted AGA triloop. An AGU pseudo-triloop is key for IRP1 binding to the canonical ferritin IREs. RNA probes encoding the APP IRE stem loop exhibited the same high affinity binding to rhIRP1 as occurs for the H-ferritin IRE (35 pm). Intracellular iron chelation increased binding of IRP1 to the APP IRE, decreasing intracellular APP expression in SH-SY5Y cells. Functionally, shRNA knockdown of IRP1 caused increased expression of neural APP consistent with IRP1-APP IRE-driven translation.

  10. Exploring the intricate regulatory network controlling the thiazide-sensitive NaCl cotransporter (NCC).

    Science.gov (United States)

    Dimke, Henrik

    2011-12-01

    The thiazide-sensitive NaCl cotransporter (NCC) plays key roles in renal electrolyte transport and blood pressure maintenance. Regulation of this cotransporter has received increased attention recently, prompted by the discovery that mutations in the with-no-lysine (WNK) kinases are the molecular explanation for pseudohypoaldosteronism type II (PHAII). Studies suggest that WNK4 regulates NCC via two distinct pathways, depending on its state of activation. Furthermore, an intact STE20-related proline-alanine-rich kinase (SPAK)/oxidative stress response 1 kinase (OSR1) pathway was found to be necessary for a WNK4 PHAII mutation to increase NCC phosphorylation and blood pressure in mice. The mouse protein 25α is a novel regulator of the SPAK/OSR1 kinase family, which greatly increases their activity. The phosphorylation status of NCC and the WNK is regulated by the serum- and glucocorticoid-inducible kinase 1, suggesting novel mechanisms whereby aldosterone modulates NCC activity. Dephosphorylation of NCC by protein phosphatase 4 strongly influences the activity of the cotransporter, confirming an important role for NCC phosphorylation. Finally, γ-adducin increases NCC activity. This stimulatory effect is dependent on the phosphorylation status of the cotransporter. γ-Adducin only binds the dephosphorylated cotransporter, suggesting that phosphorylation of NCC causes the dissociation of γ-adducin. Since γ-adducin is not a kinase, it is tempting to speculate that the protein exerts its function by acting as a scaffold between the dephosphorylated cotransporter and the regulatory kinase. As more molecular regulators of NCC are identified, the system-controlling NCC activity is becoming increasingly complex. This intricacy confers an ability to integrate a variety of stimuli, thereby regulating NCC transport activity and ultimately blood pressure.

  11. A Regulatory Circuit Composed of a Transcription Factor, IscR, and a Regulatory RNA, RyhB, Controls Fe-S Cluster Delivery

    Directory of Open Access Journals (Sweden)

    Pierre Mandin

    2016-09-01

    Full Text Available Fe-S clusters are cofactors conserved through all domains of life. Once assembled by dedicated ISC and/or SUF scaffolds, Fe-S clusters are conveyed to their apo-targets via A-type carrier proteins (ATCs. Escherichia coli possesses four such ATCs. ErpA is the only ATC essential under aerobiosis. Recent studies reported a possible regulation of the erpA mRNA by the small RNA (sRNA RyhB, which controls the expression of many genes under iron starvation. Surprisingly, erpA has not been identified in recent transcriptomic analysis of the iron starvation response, thus bringing into question the actual physiological significance of the putative regulation of erpA by RyhB. Using an sRNA library, we show that among 26 sRNAs, only RyhB represses the expression of an erpA-lacZ translational fusion. We further demonstrate that this repression occurs during iron starvation. Using mutational analysis, we show that RyhB base pairs to the erpA mRNA, inducing its disappearance. In addition, IscR, the master regulator of Fe-S homeostasis, represses expression of erpA at the transcriptional level when iron is abundant, but depleting iron from the medium alleviates this repression. The conjunction of transcriptional derepression by IscR and posttranscriptional repression by RyhB under Fe-limiting conditions is best described as an incoherent regulatory circuit. This double regulation allows full expression of erpA at iron concentrations for which Fe-S biogenesis switches from the ISC to the SUF system. We further provide evidence that this regulatory circuit coordinates ATC usage to iron availability.

  12. Neural-Network-Based Adaptive Decentralized Fault-Tolerant Control for a Class of Interconnected Nonlinear Systems.

    Science.gov (United States)

    Li, Xiao-Jian; Yang, Guang-Hong

    2016-10-26

    This paper is concerned with the adaptive decentralized fault-tolerant tracking control problem for a class of uncertain interconnected nonlinear systems with unknown strong interconnections. An algebraic graph theory result is introduced to address the considered interconnections. In addition, to achieve the desirable tracking performance, a neural-network-based robust adaptive decentralized fault-tolerant control (FTC) scheme is given to compensate the actuator faults and system uncertainties. Furthermore, via the Lyapunov analysis method, it is proven that all the signals of the resulting closed-loop system are semiglobally bounded, and the tracking errors of each subsystem exponentially converge to a compact set, whose radius is adjustable by choosing different controller design parameters. Finally, the effectiveness and advantages of the proposed FTC approach are illustrated with two simulated examples.

  13. Self-Regulatory Deficits Associated with Unpracticed Mindfulness Strategies for Coping with Acute Pain.

    Science.gov (United States)

    Evans, Daniel R; Eisenlohr-Moul, Tory A; Button, Daniel F; Baer, Ruth A; Segerstrom, Suzanne C

    2014-01-01

    Training in mindfulness is a well-supported therapeutic strategy for pain conditions, though short-term mindfulness training for acute pain is not always effective. To explore the possibility that initial attempts at mindfulness in people without previous training may drain self-regulatory resources, the current study used a student sample (N=63) to test the hypothesis that brief instruction in mindfulness would lead to reduced pain tolerance on a cold pressor task (CPT), compared to more familiar strategies for coping with acute pain. We also investigated whether high heart rate variability (HRV), a physiological indicator of self-regulatory capacity, would predict pain tolerance. Higher HRV predicted greater pain tolerance only in the control group, suggesting that applying unfamiliar mindfulness strategies while attempting to tolerate pain more rapidly sapped self-regulatory strength.

  14. Detection and reconstruction of error control codes for engineered and biological regulatory systems.

    Energy Technology Data Exchange (ETDEWEB)

    May, Elebeoba Eni; Rintoul, Mark Daniel; Johnston, Anna Marie; Pryor, Richard J.; Hart, William Eugene; Watson, Jean-Paul

    2003-10-01

    A fundamental challenge for all communication systems, engineered or living, is the problem of achieving efficient, secure, and error-free communication over noisy channels. Information theoretic principals have been used to develop effective coding theory algorithms to successfully transmit information in engineering systems. Living systems also successfully transmit biological information through genetic processes such as replication, transcription, and translation, where the genome of an organism is the contents of the transmission. Decoding of received bit streams is fairly straightforward when the channel encoding algorithms are efficient and known. If the encoding scheme is unknown or part of the data is missing or intercepted, how would one design a viable decoder for the received transmission? For such systems blind reconstruction of the encoding/decoding system would be a vital step in recovering the original message. Communication engineers may not frequently encounter this situation, but for computational biologists and biotechnologist this is an immediate challenge. The goal of this work is to develop methods for detecting and reconstructing the encoder/decoder system for engineered and biological data. Building on Sandia's strengths in discrete mathematics, algorithms, and communication theory, we use linear programming and will use evolutionary computing techniques to construct efficient algorithms for modeling the coding system for minimally errored engineered data stream and genomic regulatory DNA and RNA sequences. The objective for the initial phase of this project is to construct solid parallels between biological literature and fundamental elements of communication theory. In this light, the milestones for FY2003 were focused on defining genetic channel characteristics and providing an initial approximation for key parameters, including coding rate, memory length, and minimum distance values. A secondary objective addressed the question of

  15. TNFRp55 controls regulatory T cell responses in Yersinia-induced reactive arthritis.

    Science.gov (United States)

    Cargnelutti, Ethelina; Arias, José L; Valdez, Susana R; Rabinovich, Gabriel A; Di Genaro, María S

    2013-02-01

    In addition to its well-known pro-inflammatory effects, tumor necrosis factor (TNF) displays anti-inflammatory activities through mechanisms poorly understood. Previously, we reported the development of severe chronic Yersinia enterocolitica-induced reactive arthritis (ReA) in mice lacking the TNF receptor (TNFR)p55. As regulatory T (T(reg)) cells limit chronic inflammation, here we aim to investigate the expansion and function of CD4(+)CD25(+)FoxP3(+) T(reg) cells in the ReA animal model. The number of T(reg) cells as well as the FoxP3 mRNA expression and interleukin (IL)-10 levels were significantly decreased in joint regional lymph nodes (RLNs) of TNFRp55(-/-) mice vs wild-type (WT) mice at the arthritis onset. However, at chronic phase of arthritis, the number of T(reg) cell in TNFRp55(-/-) was similar to WT mice. To explore the in vivo function of T(reg) cells at this chronic phase in WT and TNFRp55-deficient mice, we adoptively transferred CD4(+) T cells from TNFRp55-deficient mice of day 21, into naïve WT or TNFRp55(-/-) mice. When knockout mice were used as recipients we observed higher delayed-type hypersensitivity (DTH) responses and joint inflammation after heat-killed Yersinia (HKY) stimulation. Accordingly, we found higher levels of IL-17, interferon (IFN)-γ, IL-6, transforming growth factor (TGF)-β1 and IL-12/23p40 and lower IL-10 levels in RLN of paws challenged with HKY in TNFRp55(-/-) recipient mice. In addition, we found that CD4(+) T cells from TNFRp55(-/-) mice controlled antigen-specific IL-12/23(p40) production in recipient WT mice. Our results show that TNFRp55 controls the induction and function of T(reg) cells through differential regulation of cytokine production, suggesting a novel molecular target for immune intervention in ReA.

  16. Iron regulatory proteins control a mucosal block to intestinal iron absorption.

    Science.gov (United States)

    Galy, Bruno; Ferring-Appel, Dunja; Becker, Christiane; Gretz, Norbert; Gröne, Hermann-Josef; Schümann, Klaus; Hentze, Matthias W

    2013-03-28

    Mammalian iron metabolism is regulated systemically by the hormone hepcidin and cellularly by iron regulatory proteins (IRPs) that orchestrate a posttranscriptional regulatory network. Through ligand-inducible genetic ablation of both IRPs in the gut epithelium of adult mice, we demonstrate that IRP deficiency impairs iron absorption and promotes mucosal iron retention via a ferritin-mediated "mucosal block." We show that IRP deficiency does not interfere with intestinal sensing of body iron loading and erythropoietic iron need, but rather alters the basal expression of the iron-absorption machinery. IRPs thus secure sufficient iron transport across absorptive enterocytes by restricting the ferritin "mucosal block" and define a basal set point for iron absorption upon which IRP-independent systemic regulatory inputs are overlaid.

  17. Iron Regulatory Proteins Control a Mucosal Block to Intestinal Iron Absorption

    Directory of Open Access Journals (Sweden)

    Bruno Galy

    2013-03-01

    Full Text Available Mammalian iron metabolism is regulated systemically by the hormone hepcidin and cellularly by iron regulatory proteins (IRPs that orchestrate a posttranscriptional regulatory network. Through ligand-inducible genetic ablation of both IRPs in the gut epithelium of adult mice, we demonstrate that IRP deficiency impairs iron absorption and promotes mucosal iron retention via a ferritin-mediated “mucosal block.” We show that IRP deficiency does not interfere with intestinal sensing of body iron loading and erythropoietic iron need, but rather alters the basal expression of the iron-absorption machinery. IRPs thus secure sufficient iron transport across absorptive enterocytes by restricting the ferritin “mucosal block” and define a basal set point for iron absorption upon which IRP-independent systemic regulatory inputs are overlaid.

  18. Weed control changes and genetically modified herbicide tolerant crops in the USA 1996–2012

    Science.gov (United States)

    Brookes, Graham

    2014-01-01

    Crops that have been genetically modified (GM) to be tolerant to herbicides have been widely grown in the USA since 1996. The rapid and widespread adoption of this technology reflects the important economic and environmental benefits that farmers have derived from its use (equal to $21.7 billion additional farm income and a 225 million kg reduction in herbicide active ingredient use 1996–2012). During this time, weed control practices in these crops relative to the ‘conventional alternative’ have evolved to reflect experience of using the technology, the challenges that have arisen and the increasing focus in recent years on developing sustainable production systems. This paper examines the evidence on the changing nature of herbicides used with these crops and in particular how farmers addressed the challenge of weed resistance. The evidence shows that use of the technology has resulted in a net reduction in both the amount of herbicide used and the associated environmental impact, as measured by the EIQ indicator when compared to what can reasonably be expected if the area planted to GM HT crops reverted to conventional production methods. It also facilitated many farmers being able to derive the economic and environmental benefits associated with switching from a plough-based to a no tillage or conservation tillage production system. In terms of herbicide use, the technology has also contributed to a change the profile of herbicides used. A broad range of, mostly selective herbicides has been replaced by one or 2 broad-spectrum herbicides (mostly glyphosate) used in conjunction with one or 2 other (complementary) herbicides. Since the mid-2000s, the average amount of herbicide applied and the associated environmental load, as measured by the EIQ indicator, have increased on both GM HT and conventional crops. A primary reason for these changes has been increasing incidence of weed species developing populations resistant to herbicides and increased

  19. Weed control changes and genetically modified herbicide tolerant crops in the USA 1996-2012.

    Science.gov (United States)

    Brookes, Graham

    2014-01-01

    Crops that have been genetically modified (GM) to be tolerant to herbicides have been widely grown in the USA since 1996. The rapid and widespread adoption of this technology reflects the important economic and environmental benefits that farmers have derived from its use (equal to $21.7 billion additional farm income and a 225 million kg reduction in herbicide active ingredient use 1996-2012). During this time, weed control practices in these crops relative to the 'conventional alternative' have evolved to reflect experience of using the technology, the challenges that have arisen and the increasing focus in recent years on developing sustainable production systems. This paper examines the evidence on the changing nature of herbicides used with these crops and in particular how farmers addressed the challenge of weed resistance. The evidence shows that use of the technology has resulted in a net reduction in both the amount of herbicide used and the associated environmental impact, as measured by the EIQ indicator when compared to what can reasonably be expected if the area planted to GM HT crops reverted to conventional production methods. It also facilitated many farmers being able to derive the economic and environmental benefits associated with switching from a plough-based to a no tillage or conservation tillage production system. In terms of herbicide use, the technology has also contributed to a change the profile of herbicides used. A broad range of, mostly selective herbicides has been replaced by one or 2 broad-spectrum herbicides (mostly glyphosate) used in conjunction with one or 2 other (complementary) herbicides. Since the mid-2000s, the average amount of herbicide applied and the associated environmental load, as measured by the EIQ indicator, have increased on both GM HT and conventional crops. A primary reason for these changes has been increasing incidence of weed species developing populations resistant to herbicides and increased awareness of

  20. Influence of oxygen on DNA binding, positive control, and stability of the Bradyrhizobium japonicum NifA regulatory protein.

    OpenAIRE

    1991-01-01

    Central to the genetic regulatory circuit that controls Bradyrhizobium japonicum nif and fix gene expression is the NifA protein. NifA activates transcription of several nif and fix genes and autoregulates its expression during symbiosis in soybean root nodules or in free-living microaerobic conditions. High O2 tensions result in the lack of nif expression, possibly by inactivation of NifA through oxidation of an essential metal cofactor. Several B. japonicum nif and fix promoters have upstre...

  1. 网络化控制系统随机容错控制%Stochastic Fault-Tolerant Control of Networked Control Systems

    Institute of Scientific and Technical Information of China (English)

    刘自鑫; 钟守铭

    2011-01-01

    Considering the random failures of controller and actuator, by introducing a switching matrix descriped by independent Bernoulli random sequence, a stochastic networked control model is established. And the problem of random fault-tolerant control with the existence of sensor failure and controller failure for this constructed model is investigated. Combined with linear matrix inequality technology and Lyapunov stability theory, exponential stability criteria in mean square are obtained. Based on the obtained criteria, random fault-tolerant controllers are designed. The numerical example of a controller with feedback control and fault-tolerant control is given. Simulation example shows that the design method is valid.%考虑控制器及执行器失效的随机性,通过引入具有独立Bernoulli分布随机序列开关矩阵,建立了网络化控制系统随机模型,并研究了该模型存在传感器随机失效、控制器随机失效以及二者同时存在随机失效的随机容错控制问题.

  2. Efficacy and tolerability of desvenlafaxine succinate treatment for menopausal vasomotor symptoms: a randomized controlled trial.

    Science.gov (United States)

    Speroff, Leon; Gass, Margery; Constantine, Ginger; Olivier, Sophie

    2008-01-01

    To compare efficacy and safety of desvenlafaxine succinate (desvenlafaxine) with placebo for the treatment of vasomotor symptoms. This randomized, double-blind, placebo-controlled trial enrolled 707 healthy, postmenopausal women experiencing 50 or more moderate-to-severe hot flushes per week. Participants randomly received desvenlafaxine 50, 100, 150, or 200 mg or placebo daily. Trial duration was 52 weeks. Primary outcomes were change from baseline in average daily number of moderate-to-severe hot flushes and in daily hot flush severity score at weeks 4 and 12. Six hundred twenty women with an average of 11 moderate-to-severe hot flushes per day at baseline completed at least one on-therapy evaluation for primary efficacy end points; 519 participants completed 12 weeks of treatment, and 368 completed the study. Desvenlafaxine 100 mg/d achieved a significantly greater reduction compared with placebo in average daily number of hot flushes at weeks 4 (P=.013) and 12 (P=.005), reaching a 64% decrease from baseline at week 12, and the 75% responder rate was significantly higher for desvenlafaxine 100 mg (50%) compared with placebo (29%; P=.003; number needed to treat=4.7) at week 12. Average daily severity of hot flushes was significantly lower in the desvenlafaxine 100-mg group compared with placebo at week 12 (P=.020). Desvenlafaxine-treated women reported significantly more treatment-emergent adverse events than placebo-treated women during the first week of therapy only. Desvenlafaxine is an effective nonhormonal treatment for vasomotor symptoms in postmenopausal women. Its tolerability profile is consistent with that of other serotonin-norepinephrine reuptake inhibitors. ClinicalTrials.gov, www.clinicaltrials.gov, NCT00421031 I.

  3. Desenho e funcionamento dos mecanismos de controle e accountability das agências reguladoras brasileiras: semelhanças e diferenças Design and operation of the Brazilian regulatory agencies' control and accountability mechanisms: similarities and differences

    National Research Council Canada - National Science Library

    Marcos Vinicius Pó; Fernando Luiz Abrucio

    2006-01-01

    ... agências reguladoras provoca na accountability do Estado brasileiro.This article studies the design and operation of the regulatory agencies' control and accountability mechanisms, underlining...

  4. Child attention to pain and pain tolerance are dependent upon anxiety and attention control: An eye-tracking study.

    Science.gov (United States)

    Heathcote, L C; Lau, J Y F; Mueller, S C; Eccleston, C; Fox, E; Bosmans, M; Vervoort, T

    2017-02-01

    Pain is common and can be debilitating in childhood. Theoretical models propose that attention to pain plays a key role in pain outcomes, however, very little research has investigated this in youth. This study examined how anxiety-related variables and attention control interacted to predict children's attention to pain cues using eye-tracking methodology, and their pain tolerance on the cold pressor test (CPT). Children aged 8-17 years had their eye-gaze tracked whilst they viewed photographs of other children displaying painful facial expressions during the CPT, before completing the CPT themselves. Children also completed self-report measures of anxiety and attention control. Findings indicated that anxiety and attention control did not impact children's initial fixations on pain or neutral faces, but did impact how long they dwelled on pain versus neutral faces. For children reporting low levels of attention control, higher anxiety was associated with less dwell time on pain faces as opposed to neutral faces, and the opposite pattern was observed for children with high attention control. Anxiety and attention control also interacted to predict pain outcomes. For children with low attention control, increasing anxiety was associated with anticipating more pain and tolerating pain for less time. This is the first study to examine children's attention to pain cues using eye-tracking technology in the context of a salient painful experience. Data suggest that attention control is an important moderator of anxiety on multiple outcomes relevant to young people's pain experiences. This study uses eye tracking to study attention to pain cues in children. Attention control is an important moderator of anxiety on attention bias to pain and tolerance of cold pressor pain in youth. © 2016 European Pain Federation - EFIC®.

  5. Quantitative sensory testing and pain tolerance in patients with mild to moderate Alzheimer's disease compared to healthy control

    DEFF Research Database (Denmark)

    Jensen-Dahm, Christina; Werner, Mads Utke; Dahl, Jørgen Berg;

    2014-01-01

    Patients with Alzheimer disease (AD) report pain less frequently than their cognitively intact peers. It has been hypothesized that pain processing is altered in AD. The aim of this study was to investigate agreement and reliability of 3 pain sensitivity tests and to examine pain threshold...... and tolerance in patients with AD. We examined 29 patients with mild to moderate AD and 29 age- and gender-matched healthy control subjects with quantitative sensory testing, ie, assessments of detection threshold (warmth detection threshold [WDT]) and pain threshold (heat pain threshold [HPT], pressure...... algometry, cold pressor test), and assessments of tolerance (pressure algometry, cold pressor test). All procedures were done twice on day 1, 1 hour apart, and repeated on day 2. We found no difference between groups for WDT (patient vs control subjects: mean [95% confidence interval]: 35.5°C [33.4°C to 37...

  6. Reliability Improvement of a T-Type Three-Level Inverter With Fault-Tolerant Control Strategy

    DEFF Research Database (Denmark)

    Choi, Uimin; Blaabjerg, Frede; Lee, Kyo-Beum

    2015-01-01

    in a neutral-point switch, two methods will be proposed and compared based on thermal analysis and neutral-point voltage oscillation. The reliability of T-type inverter systems is improved considerably by the proposed algorithm when a switch fails. The proposed method does not require any additional components......This paper proposes a fault-tolerant control strategy for a T-type three-level inverter when an open-circuit fault occurs. The proposed method is explained by dividing fault into two cases: the faulty condition of half-bridge switches and neutral-point switches. In case of the open-circuit fault....... Simulation and experimental results verify the validity and feasibility of the proposed fault-tolerant control strategy....

  7. Fault diagnosis and fault-tolerant finite control set-model predictive control of a multiphase voltage-source inverter supplying BLDC motor.

    Science.gov (United States)

    Salehifar, Mehdi; Moreno-Equilaz, Manuel

    2016-01-01

    Due to its fault tolerance, a multiphase brushless direct current (BLDC) motor can meet high reliability demand for application in electric vehicles. The voltage-source inverter (VSI) supplying the motor is subjected to open circuit faults. Therefore, it is necessary to design a fault-tolerant (FT) control algorithm with an embedded fault diagnosis (FD) block. In this paper, finite control set-model predictive control (FCS-MPC) is developed to implement the fault-tolerant control algorithm of a five-phase BLDC motor. The developed control method is fast, simple, and flexible. A FD method based on available information from the control block is proposed; this method is simple, robust to common transients in motor and able to localize multiple open circuit faults. The proposed FD and FT control algorithm are embedded in a five-phase BLDC motor drive. In order to validate the theory presented, simulation and experimental results are conducted on a five-phase two-level VSI supplying a five-phase BLDC motor.

  8. Global regulatory networks control the hrp regulon of the gall-forming bacterium Pantoea agglomerans pv. gypsophilae.

    Science.gov (United States)

    Panijel, Mary; Chalupowicz, Laura; Sessa, Guido; Manulis-Sasson, Shulamit; Barash, Isaac

    2013-09-01

    Gall formation by Pantoea agglomerans pv. gypsophilae is dependent on the hypersensitive response and pathogenicity (hrp) system. Previous studies demonstrated that PagR and PagI, regulators of the quorum-sensing system, induce expression of the hrp regulatory cascade (i.e., hrpXY, hrpS, and hrpL) that activates the HrpL regulon. Here, we isolated the genes of the Gac/Rsm global regulatory pathway (i.e., gacS, gacA, rsmB, and csrD) and of the post-transcriptional regulator rsmA. Our results demonstrate that PagR and PagI also upregulate expression of the Gac/Rsm pathway. PagR acts as a transcriptional activator of each of the hrp regulatory genes and gacA in a N-butanoyl-L-homoserine lactone-dependent manner as shown by gel shift experiments. Mutants of the Gac/Rsm genes or overexpression of rsmA significantly reduced Pantoea agglomerans virulence and colonization of gypsophila. Overexpression of rsmB sRNA abolished gall formation, colonization, and hypersensitive reaction on nonhost plants and prevented transcription of the hrp regulatory cascade, indicating a lack of functional type III secretion system. Expression of rsmB sRNA in the background of the csrD null mutant suggests that CsrD may act as a safeguard for preventing excessive production of rsmB sRNA. Results presented indicate that the hrp regulatory cascade is controlled directly by PagR and indirectly by RsmA, whereas deficiency in RsmA activity is epistatic to PagR induction.

  9. Validation Methods Research for Fault-Tolerant Avionics and Control Systems Sub-Working Group Meeting. CARE 3 peer review

    Science.gov (United States)

    Trivedi, K. S. (Editor); Clary, J. B. (Editor)

    1980-01-01

    A computer aided reliability estimation procedure (CARE 3), developed to model the behavior of ultrareliable systems required by flight-critical avionics and control systems, is evaluated. The mathematical models, numerical method, and fault-tolerant architecture modeling requirements are examined, and the testing and characterization procedures are discussed. Recommendations aimed at enhancing CARE 3 are presented; in particular, the need for a better exposition of the method and the user interface is emphasized.

  10. Self-consistent theory of transcriptional control in complex regulatory architectures

    NARCIS (Netherlands)

    Landman, Jasper; Brewster, Robert C; Weinert, Franz M; Phillips, Rob; Kegel, Willem K.|info:eu-repo/dai/nl/113729464

    2017-01-01

    Individual regulatory proteins are typically charged with the simultaneous regulation of a battery of different genes. As a result, when one of these proteins is limiting, competitive effects have a significant impact on the transcriptional response of the regulated genes. Here we present a general

  11. Regulatory risk control through mandatory occupational safety and health (OSH) certification and testing regimes (CTRs)

    NARCIS (Netherlands)

    Zwetsloot, G.I.J.M.; Hale, A.; Zwanikken, S.

    2011-01-01

    Governments make increasing use of private certification and testing infrastructures as an alternative for traditional regulatory arrangements in several areas including occupational safety and health (OSH).This research, commissioned by the Dutch Inspectorate for Work and Income (IWI), concerns an

  12. Small RNA-Controlled Gene Regulatory Networks in Pseudomonas putida

    DEFF Research Database (Denmark)

    Bojanovic, Klara

    evolved numerous mechanisms to controlgene expression in response to specific environmental signals. In addition to two-component systems, small regulatory RNAs (sRNAs) have emerged as major regulators of gene expression. The majority of sRNAs bind to mRNA and regulate their expression. They often have...

  13. Regulatory risk control through mandatory occupational safety and health (OSH) certification and testing regimes (CTRs)

    NARCIS (Netherlands)

    Zwetsloot, G.I.J.M.; Hale, A.; Zwanikken, S.

    2011-01-01

    Governments make increasing use of private certification and testing infrastructures as an alternative for traditional regulatory arrangements in several areas including occupational safety and health (OSH).This research, commissioned by the Dutch Inspectorate for Work and Income (IWI), concerns an

  14. Tolerating Zero Tolerance?

    Science.gov (United States)

    Moore, Brian N.

    2010-01-01

    The concept of zero tolerance dates back to the mid-1990s when New Jersey was creating laws to address nuisance crimes in communities. The main goal of these neighborhood crime policies was to have zero tolerance for petty crime such as graffiti or littering so as to keep more serious crimes from occurring. Next came the war on drugs. In federal…

  15. Self-consistent theory of transcriptional control in complex regulatory architectures

    Science.gov (United States)

    Brewster, Robert C.; Weinert, Franz M.; Phillips, Rob; Kegel, Willem K.

    2017-01-01

    Individual regulatory proteins are typically charged with the simultaneous regulation of a battery of different genes. As a result, when one of these proteins is limiting, competitive effects have a significant impact on the transcriptional response of the regulated genes. Here we present a general framework for the analysis of any generic regulatory architecture that accounts for the competitive effects of the regulatory environment by isolating these effects into an effective concentration parameter. These predictions are formulated using the grand-canonical ensemble of statistical mechanics and the fold-change in gene expression is predicted as a function of the number of transcription factors, the strength of interactions between the transcription factors and their DNA binding sites, and the effective concentration of the transcription factor. The effective concentration is set by the transcription factor interactions with competing binding sites within the cell and is determined self-consistently. Using this approach, we analyze regulatory architectures in the grand-canonical ensemble ranging from simple repression and simple activation to scenarios that include repression mediated by DNA looping of distal regulatory sites. It is demonstrated that all the canonical expressions previously derived in the case of an isolated, non-competing gene, can be generalised by a simple substitution to their grand canonical counterpart, which allows for simple intuitive incorporation of the influence of multiple competing transcription factor binding sites. As an example of the strength of this approach, we build on these results to present an analytical description of transcriptional regulation of the lac operon. PMID:28686609

  16. Validation Methods Research for Fault-Tolerant Avionics and Control Systems: Working Group Meeting, 2

    Science.gov (United States)

    Gault, J. W. (Editor); Trivedi, K. S. (Editor); Clary, J. B. (Editor)

    1980-01-01

    The validation process comprises the activities required to insure the agreement of system realization with system specification. A preliminary validation methodology for fault tolerant systems documented. A general framework for a validation methodology is presented along with a set of specific tasks intended for the validation of two specimen system, SIFT and FTMP. Two major areas of research are identified. First, are those activities required to support the ongoing development of the validation process itself, and second, are those activities required to support the design, development, and understanding of fault tolerant systems.

  17. Rate setting and regulatory control of the radionuclides content in foodstuffs

    Energy Technology Data Exchange (ETDEWEB)

    Shandala, N. K.; Savskin, M. N.; Petukhova, E. V.; Novikova, N. Y.; Titov, A. V.

    2004-07-01

    The concept of rate setting, standardisation and regulatory control of the radionuclides content in foodstuffs consists in the following: ? the normative standards concern to 90Sr and 137Cs as biologically significant radionuclides; ? the internal exposure dose from ingestion intake of radionuclides with food should not exceed meaning of 1 mSv a-1 which is the intervention exception level concerning trading foodstuffs; ? for few consumed foodstuffs (wild berries, mushrooms, teas) is established, that the dose caused by their consumption is outside of dose limits of 1 mSv a-1 and it should not exceed 1 % for each product; ? the nutrition structure of population including seven basic components of a diet (bread and grain products, dairy products, potatoes, vegetables, meat, fish, fruit and berries) is taken into account as a source of ingestion intake of the radionuclides; ? The conformity to the established standard is determined on a parameter ?f the summary contents of 90Sr and 137Cs in foodstuff. With the purpose of the operative control of an internal exposure of population the permissible levels of specific activity of 90Sr and 137Cs in more than 120 kinds of foodstuff were developed. These standards are obligatory in Russia for domestic and imported foodstuffs. So, permissible levels of specific activity of 90Sr and 137Cs in bread make, accordingly, 20 and 40 Bq kg-1; in milk - 25 and 100 Bq kg-1; in meat - 50 and 160 Bq kg-1; in potatoes and vegetables - 40 and 120 Bq kg-1. Monitoring of contamination of foodstuffs by 137Cs and 90Sr is one of the basic measures on maintenance of radiation safety of population in Russia. State system of sanitary-epidemiological control carries out constant supervision over the content of the radionuclides in food production. By the carried out researches was shown that true levels of the contents of 90Sr or 137Cs in the main foodstuffs in territory of Russia, except for the territories, injures of the radiation accidents, make

  18. Robust Fault-Tolerant Control for Satellite Attitude Stabilization Based on Active Disturbance Rejection Approach with Artificial Bee Colony Algorithm

    Directory of Open Access Journals (Sweden)

    Fei Song

    2014-01-01

    Full Text Available This paper proposed a robust fault-tolerant control algorithm for satellite stabilization based on active disturbance rejection approach with artificial bee colony algorithm. The actuating mechanism of attitude control system consists of three working reaction flywheels and one spare reaction flywheel. The speed measurement of reaction flywheel is adopted for fault detection. If any reaction flywheel fault is detected, the corresponding fault flywheel is isolated and the spare reaction flywheel is activated to counteract the fault effect and ensure that the satellite is working safely and reliably. The active disturbance rejection approach is employed to design the controller, which handles input information with tracking differentiator, estimates system uncertainties with extended state observer, and generates control variables by state feedback and compensation. The designed active disturbance rejection controller is robust to both internal dynamics and external disturbances. The bandwidth parameter of extended state observer is optimized by the artificial bee colony algorithm so as to improve the performance of attitude control system. A series of simulation experiment results demonstrate the performance superiorities of the proposed robust fault-tolerant control algorithm.

  19. Moderating Effect of Self-Regulatory Efficacy on the Relationship between Organizational Formal Controls and Workplace Deviance: A Proposed Framework

    Directory of Open Access Journals (Sweden)

    Kabiru Maitama Kura

    2013-01-01

    Full Text Available Several studies in the field of industrial and organizational psychology (I/O have reported that workplace deviance is related to organization/work variables, such as organizational politics, perceived organizational support, job satisfaction, job stress, and organizational justice among others. However, relatively few studies have attempted to consider the relationship between organizational formal controls and workplace deviance. Even if any, they have reported mixed findings. Hence, a moderating variable is suggested. This paper proposes a moderating role of self-regulatory efficacy on the relationship between organizational formal controls and workplace deviance.

  20. Illegal, Unreported and Unregulated Fishing Control in the Exclusive Economic Zone: a Brief Appraisal of Regulatory Deficits and Accountability Strategies

    Directory of Open Access Journals (Sweden)

    Rosello Mercedes

    2016-08-01

    Full Text Available The conservation of fish stocks in the world’s exclusive economic zones (EEZs, which collectively harbour the vast majority of marine-living resources, is the primary responsibility of coastal States. As the effects of failures by coastal States to protect those stocks from the impacts of illegal, unreported and unregulated (IUU fishing may extend beyond domestic boundaries, this paper questions whether and how coastal States may be made accountable in respect of their regulatory deficits. With the proliferation of non-legal conduct rules to guide the regulatory role of States and their agencies, non-judicial mechanisms have the potential to foster coastal State stewardship of domestic fisheries. Outlining a number of international, transnational and domestic approaches, this paper gives consideration to the opportunities and limitations they present in order to strengthen coastal State accountability for IUU fishing control deficits.

  1. Role of the Pi3k Regulatory Subunit in the Control of Actin Organization and Cell Migration

    Science.gov (United States)

    Jiménez, Concepción; Portela, Rosario Armas; Mellado, Mario; Rodríguez-Frade, Jose Miguel; Collard, John; Serrano, Antonio; Martínez-A, Carlos; Avila, Jesus; Carrera, Ana C.

    2000-01-01

    Cell migration represents an important cellular response that utilizes cytoskeletal reorganization as its driving force. Here, we describe a new signaling cascade linking PDGF receptor stimulation to actin rearrangements and cell migration. We demonstrate that PDGF activates Cdc42 and its downstream effector N-WASP to mediate filopodia formation, actin stress fiber disassembly, and a reduction in focal adhesion complexes. Induction of the Cdc42 pathway is independent of phosphoinositide 3-kinase (PI3K) enzymatic activity, but it is dependent on the p85α regulatory subunit of PI3K. Finally, data are provided showing that activation of this pathway is required for PDGF-induced cell migration on collagen. These observations show the essential role of the PI3K regulatory subunit p85α in controlling PDGF receptor–induced cytoskeletal changes and cell migration, illustrating a novel signaling pathway that links receptor stimulation at the cell membrane with actin dynamics. PMID:11038173

  2. Breath holding duration as a measure of distress tolerance: examining its relation to measures of executive control.

    Science.gov (United States)

    Sütterlin, Stefan; Schroijen, Mathias; Constantinou, Elena; Smets, Elyn; Van den Bergh, Omer; Van Diest, Ilse

    2013-01-01

    Recent research considers distress (in)tolerance as an essential component in the development of various forms of psychopathology. A behavioral task frequently used to assess distress tolerance is the breath holding task. Although breath holding time (BHT) has been associated with behavioral outcomes related to inhibitory control (e.g., smoking cessation), the relationship among breath holding and direct measures of executive control has not yet been thoroughly examined. The present study aims to assess (a) the BHT-task's test-retest reliability in a 1-year follow-up and (b) the relationship between a series of executive function tasks and breath holding duration. One hundred and thirteen students completed an initial BHT assessment, 58 of which also completed a series of executive function tasks [the Wisconsin Card Sorting Test (WCST), the Parametric Go/No-Go task and the N-back memory updating task]. A subsample of these students (N = 34) repeated the breath holding task in a second session 1 year later. Test-retest reliability of the BHT-task over a 1-year period was high (r = 0.67, p BHT. The rather moderate levels of unpleasantness induced by breath holding in our sample may suggest that other processes (physiological, motivational) besides distress tolerance influence BHT. Overall, the current findings do not support the assumption of active inhibitory control in the BHT-task in a healthy sample. Our findings suggest that individual differences (e.g., in interoceptive or anxiety sensitivity) should be taken into account when examining the validity of BHT as a measure of distress tolerance.

  3. Implementation of fault tolerant control for modular multilevel converter using EtherCAT communication

    DEFF Research Database (Denmark)

    Burlacu, Paul Dan; Mathe, Laszlo; Rejas, Marcos;

    2015-01-01

    . This communication platform has to ensure a perfect synchronization between the modules, and it should be also fault tolerant. The analysis of a MMC based on EtherCAT is presented in this paper from implementation and module fault point of view. The experimental tests show that the MMC operates after communication...

  4. Melatonin for Sleep in Children with Autism: A Controlled Trial Examining Dose, Tolerability, and Outcomes

    Science.gov (United States)

    Malow, Beth; Adkins, Karen W.; McGrew, Susan G.; Wang, Lily; Goldman, Suzanne E.; Fawkes, Diane; Burnette, Courtney

    2012-01-01

    Supplemental melatonin has shown promise in treating sleep onset insomnia in children with autism spectrum disorders (ASD). Twenty-four children, free of psychotropic medications, completed an open-label dose-escalation study to assess dose-response, tolerability, safety, feasibility of collecting actigraphy data, and ability of outcome measures…

  5. Fault tolerant control with torque limitation based on fault mode for ten-phase permanent magnet synchronous motor

    Institute of Scientific and Technical Information of China (English)

    Guo Hong; Xu Jinquan

    2015-01-01

    This paper proposes a novel fault tolerant control with torque limitation based on the fault mode for the ten-phase permanent magnet synchronous motor (PMSM) under various open-circuit and short-circuit fault conditions, which includes the optimal torque control and the torque limitation control based on the fault mode. The optimal torque control is adopted to guarantee the ripple-free electromagnetic torque operation for the ten-phase motor system under the post-fault condition. Furthermore, we systematically analyze the load capacity of the ten-phase motor system under different fault modes. And a torque limitation control approach based on the fault mode is proposed, which was not available earlier. This approach is able to ensure the safety operation of the faulted motor system in long operating time without causing the overheat fault. The simulation result confirms that the proposed fault tolerant control for the ten-phase motor system is able to guarantee the ripple-free electromagnetic torque and the safety operation in long operating time under the normal and fault conditions.

  6. Fault tolerant control with torque limitation based on fault mode for ten-phase permanent magnet synchronous motor

    Directory of Open Access Journals (Sweden)

    Guo Hong

    2015-10-01

    Full Text Available This paper proposes a novel fault tolerant control with torque limitation based on the fault mode for the ten-phase permanent magnet synchronous motor (PMSM under various open-circuit and short-circuit fault conditions, which includes the optimal torque control and the torque limitation control based on the fault mode. The optimal torque control is adopted to guarantee the ripple-free electromagnetic torque operation for the ten-phase motor system under the post-fault condition. Furthermore, we systematically analyze the load capacity of the ten-phase motor system under different fault modes. And a torque limitation control approach based on the fault mode is proposed, which was not available earlier. This approach is able to ensure the safety operation of the faulted motor system in long operating time without causing the overheat fault. The simulation result confirms that the proposed fault tolerant control for the ten-phase motor system is able to guarantee the ripple-free electromagnetic torque and the safety operation in long operating time under the normal and fault conditions.

  7. Transcriptional control of yeast ribosome biogenesis: A multifaceted role for general regulatory factors.

    Science.gov (United States)

    Bosio, Maria Cristina; Fermi, Beatrice; Dieci, Giorgio

    2017-08-08

    In Saccharomyces cerevisiae, a group of more than 200 co-regulated genes (Ribi genes) is involved in ribosome biogenesis. This regulon has recently been shown to rely on a small set of transcriptional regulators (mainly Abf1, but also Reb1, Tbf1 and Rap1) previously referred to as general regulatory factors (GRFs) because of their widespread binding and action at many promoters and other specialized genomic regions. Intriguingly, Abf1 binding to Ribi genes is differentially modulated in response to distinct nutrition signaling pathways. Such a dynamic promoter association has the potential to orchestrate both activation and repression of Ribi genes in synergy with neighboring regulatory sites and through the functional interplay of histone acetyltransferases and deacetylases.

  8. Physiological and regulatory effects of controlled overproduction of five cold shock proteins of Lactococcus lactis

    NARCIS (Netherlands)

    Wouters, J.A.; Mailhes, M.; Rombouts, F.M.; Vos, de W.M.; Kuipers, O.P.; Abee, T.

    2000-01-01

    The physiological and regulatory effects of overproduction of five cold shock proteins (CSPs) of Lactococcus lactis were studied. CspB, CspD, and CspE could be overproduced at high levels (up to 19␘f the total protein), whereas for CspA and CspC limited overproduction (0.3 to 0.5␘f the total protein

  9. Auto and cross regulatory elements control Onecut expression in the ascidian nervous system.

    Science.gov (United States)

    Pezzotti, Maria Rosa; Locascio, Annamaria; Racioppi, Claudia; Fucci, Laura; Branno, Margherita

    2014-06-15

    The expression pattern of Onecut genes in the central and peripheral nervous systems is highly conserved in invertebrates and vertebrates but the regulatory networks in which they are involved are still largely unknown. The presence of three gene copies in vertebrates has revealed the functional roles of the Onecut genes in liver, pancreas and some populations of motor neurons. Urochordates have only one Onecut gene and are the closest living relatives of vertebrates and thus represent a good model system to understand its regulatory network and involvement in nervous system formation. In order to define the Onecut genetic cascade, we extensively characterized the Onecut upstream cis-regulatory DNA in the ascidian Ciona intestinalis. Electroporation experiments using a 2.5kb genomic fragment and of a series of deletion constructs identified a small region of 262bp able to reproduce most of the Onecut expression profile during embryonic development. Further analyses, both bioinformatic and in vivo using transient transgenes, permitted the identification of transcription factors responsible for Onecut endogenous expression. We provide evidence that Neurogenin is a direct activator of Onecut and that an autoregulatory loop is responsible for the maintenance of its expression. Furthermore, for the first time we propose the existence of a direct connection among Neurogenin, Onecut and Rx transcription factors in photoreceptor cell formation.

  10. The Caenorhabditis elegans vulva: a post-embryonic gene regulatory network controlling organogenesis.

    Science.gov (United States)

    Ririe, Ted O; Fernandes, Jolene S; Sternberg, Paul W

    2008-12-23

    The Caenorhabditis elegans vulva is an elegant model for dissecting a gene regulatory network (GRN) that directs postembryonic organogenesis. The mature vulva comprises seven cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), each with its own unique pattern of spatial and temporal gene expression. The mechanisms that specify these cell types in a precise spatial pattern are not well understood. Using reverse genetic screens, we identified novel components of the vulval GRN, including nhr-113 in vulA. Several transcription factors (lin-11, lin-29, cog-1, egl-38, and nhr-67) interact with each other and act in concert to regulate target gene expression in the diverse vulval cell types. For example, egl-38 (Pax2/5/8) stabilizes the vulF fate by positively regulating vulF characteristics and by inhibiting characteristics associated with the neighboring vulE cells. nhr-67 and egl-38 regulate cog-1, helping restrict its expression to vulE. Computational approaches have been successfully used to identify functional cis-regulatory motifs in the zmp-1 (zinc metalloproteinase) promoter. These results provide an overview of the regulatory network architecture for each vulval cell type.

  11. Decentralized Sliding Mode Observer Based Dual Closed-Loop Fault Tolerant Control for Reconfigurable Manipulator against Actuator Failure.

    Science.gov (United States)

    Zhao, Bo; Li, Chenghao; Liu, Derong; Li, Yuanchun

    2015-01-01

    This paper considers a decentralized fault tolerant control (DFTC) scheme for reconfigurable manipulators. With the appearance of norm-bounded failure, a dual closed-loop trajectory tracking control algorithm is proposed on the basis of the Lyapunov stability theory. Characterized by the modularization property, the actuator failure is estimated by the proposed decentralized sliding mode observer (DSMO). Moreover, the actuator failure can be treated in view of the local joint information, so its control performance degradation is independent of other normal joints. In addition, the presented DFTC scheme is significantly simplified in terms of the structure of the controller due to its dual closed-loop architecture, and its feasibility is highly reflected in the control of reconfigurable manipulators. Finally, the effectiveness of the proposed DFTC scheme is demonstrated using simulations.

  12. Decentralized Sliding Mode Observer Based Dual Closed-Loop Fault Tolerant Control for Reconfigurable Manipulator against Actuator Failure.

    Directory of Open Access Journals (Sweden)

    Bo Zhao

    Full Text Available This paper considers a decentralized fault tolerant control (DFTC scheme for reconfigurable manipulators. With the appearance of norm-bounded failure, a dual closed-loop trajectory tracking control algorithm is proposed on the basis of the Lyapunov stability theory. Characterized by the modularization property, the actuator failure is estimated by the proposed decentralized sliding mode observer (DSMO. Moreover, the actuator failure can be treated in view of the local joint information, so its control performance degradation is independent of other normal joints. In addition, the presented DFTC scheme is significantly simplified in terms of the structure of the controller due to its dual closed-loop architecture, and its feasibility is highly reflected in the control of reconfigurable manipulators. Finally, the effectiveness of the proposed DFTC scheme is demonstrated using simulations.

  13. Decentralized Sliding Mode Observer Based Dual Closed-Loop Fault Tolerant Control for Reconfigurable Manipulator against Actuator Failure

    Science.gov (United States)

    Zhao, Bo; Li, Yuanchun

    2015-01-01

    This paper considers a decentralized fault tolerant control (DFTC) scheme for reconfigurable manipulators. With the appearance of norm-bounded failure, a dual closed-loop trajectory tracking control algorithm is proposed on the basis of the Lyapunov stability theory. Characterized by the modularization property, the actuator failure is estimated by the proposed decentralized sliding mode observer (DSMO). Moreover, the actuator failure can be treated in view of the local joint information, so its control performance degradation is independent of other normal joints. In addition, the presented DFTC scheme is significantly simplified in terms of the structure of the controller due to its dual closed-loop architecture, and its feasibility is highly reflected in the control of reconfigurable manipulators. Finally, the effectiveness of the proposed DFTC scheme is demonstrated using simulations. PMID:26181826

  14. A Bmp/Admp regulatory circuit controls maintenance and regeneration of dorsal-ventral polarity in planarians.

    Science.gov (United States)

    Gaviño, Michael A; Reddien, Peter W

    2011-02-22

    Animal embryos have diverse anatomy and vary greatly in size. It is therefore remarkable that a common signaling pathway, BMP signaling, controls development of the dorsoventral (DV) axis throughout the Bilateria. In vertebrates, spatially opposed expression of the BMP family proteins Bmp4 and Admp (antidorsalizing morphogenetic protein) can promote restoration of DV pattern following tissue removal. bmp4 orthologs have been identified in all three groups of the Bilateria (deuterostomes, ecdysozoans, and lophotrochozoans). By contrast, the absence of admp orthologs in ecdysozoans such as Drosophila and C. elegans has suggested that a regulatory circuit of oppositely expressed bmp4 and admp genes represents a deuterostome-specific innovation. Here we describe the existence of spatially opposed bmp and admp expression in a protostome. An admp ortholog (Smed-admp) is expressed ventrally and laterally in adult Schmidtea mediterranea planarians, opposing the dorsal-pole expression of Smed-bmp4. Smed-admp is required for regeneration following parasagittal amputation. Furthermore, Smed-admp promotes Smed-bmp4 expression and Smed-bmp4 inhibits Smed-admp expression, generating a regulatory circuit that buffers against perturbations of Bmp signaling. These results suggest that a Bmp/Admp regulatory circuit is a central feature of the Bilateria, used broadly for the establishment, maintenance, and regeneration of the DV axis.

  15. cis-Regulatory control of three cell fate-specific genes in vulval organogenesis of Caenorhabditis elegans and C. briggsae.

    Science.gov (United States)

    Kirouac, Martha; Sternberg, Paul W

    2003-05-01

    The great-grandprogeny of the Caenorhabditis elegans vulval precursor cells (VPCs) adopt one of the final vulA, B1, B2, C, D, E, and F cell fates in a precise spatial pattern. This pattern of vulval cell types is likely to depend on the cis-regulatory regions of the transcriptional targets of intercellular signals in vulval development. egl-17, zmp-1, and cdh-3 are expressed differentially in the developing vulva cells, providing a potential readout for different signaling pathways. To understand how such pathways interact to specify unique vulval cell types in a precise pattern, we have identified cis-regulatory regions sufficient to confer vulval cell type-specific regulation when fused in cis to the basal pes-10 promoter. We have identified the C. briggsae homologs of these three genes, with their corresponding control regions, and tested these regions in both C. elegans and C. briggsae. These regions of similarity in C. elegans and C. briggsae upstream of egl-17, zmp-1, and cdh-3 promote expression in vulval cells and the anchor cell (AC). By using the cis-regulatory analysis and phylogenetic footprinting, we have identified overrepresented sequences involved in conferring vulval and AC expression.

  16. Active fault tolerant control based on interval type-2 fuzzy sliding mode controller and non linear adaptive observer for 3-DOF laboratory helicopter.

    Science.gov (United States)

    Zeghlache, Samir; Benslimane, Tarak; Bouguerra, Abderrahmen

    2017-09-14

    In this paper, a robust controller for a three degree of freedom (3 DOF) helicopter control is proposed in presence of actuator and sensor faults. For this purpose, Interval type-2 fuzzy logic control approach (IT2FLC) and sliding mode control (SMC) technique are used to design a controller, named active fault tolerant interval type-2 Fuzzy Sliding mode controller (AFTIT2FSMC) based on non-linear adaptive observer to estimate and detect the system faults for each subsystem of the 3-DOF helicopter. The proposed control scheme allows avoiding difficult modeling, attenuating the chattering effect of the SMC, reducing the rules number of the fuzzy controller. Exponential stability of the closed loop is guaranteed by using the Lyapunov method. The simulation results show that the AFTIT2FSMC can greatly alleviate the chattering effect, providing good tracking performance, even in presence of actuator and sensor faults. Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.

  17. Phosphorylation of CRTC3 by the salt-inducible kinases controls the interconversion of classically activated and regulatory macrophages.

    Science.gov (United States)

    Clark, Kristopher; MacKenzie, Kirsty F; Petkevicius, Kasparas; Kristariyanto, Yosua; Zhang, Jiazhen; Choi, Hwan Geun; Peggie, Mark; Plater, Lorna; Pedrioli, Patrick G A; McIver, Ed; Gray, Nathanael S; Arthur, J Simon C; Cohen, Philip

    2012-10-16

    Macrophages acquire strikingly different properties that enable them to play key roles during the initiation, propagation, and resolution of inflammation. Classically activated (M1) macrophages produce proinflammatory mediators to combat invading pathogens and respond to tissue damage in the host, whereas regulatory macrophages (M2b) produce high levels of anti-inflammatory molecules, such as IL-10, and low levels of proinflammatory cytokines, like IL-12, and are important for the resolution of inflammatory responses. A central problem in this area is to understand how the formation of regulatory macrophages can be promoted at sites of inflammation to prevent and/or alleviate chronic inflammatory and autoimmune diseases. Here, we demonstrate that the salt-inducible kinases (SIKs) restrict the formation of regulatory macrophages and that their inhibition induces striking increases in many of the characteristic markers of regulatory macrophages, greatly stimulating the production of IL-10 and other anti-inflammatory molecules. We show that SIK inhibitors elevate IL-10 production by inducing the dephosphorylation of cAMP response element-binding protein (CREB)-regulated transcriptional coactivator (CRTC) 3, its dissociation from 14-3-3 proteins and its translocation to the nucleus where it enhances a gene transcription program controlled by CREB. Importantly, the effects of SIK inhibitors on IL-10 production are lost in macrophages that express a drug-resistant mutant of SIK2. These findings identify SIKs as a key molecular switch whose inhibition reprograms macrophages to an anti-inflammatory phenotype. The remarkable effects of SIK inhibitors on macrophage function suggest that drugs that target these protein kinases may have therapeutic potential for the treatment of inflammatory and autoimmune diseases.

  18. Why are people with high self-control happier? The effect of trait self-control on happiness as mediated by regulatory focus

    Directory of Open Access Journals (Sweden)

    Tracy T.L. Cheung

    2014-07-01

    Full Text Available Background: While self-control has often been related to positive outcomes in life such as higher academic achievements and better health, recent insights reveal that people with high trait self-control (TSC may even experience greater life satisfaction or happiness. Objective: The current study further scrutinizes this potential association between TSC and happiness, and examines how regulatory focus, which is the way people frame and direct their goal pursuit strategies, plays a role in this relationship. Accordingly, the present study examines the mediating role of regulatory-focus (promotion and prevention focus on the relationship between TSC and happiness. Method: Data was collected from 545 individuals (65.9% female, Mage = 27.52 years regarding their TSC, regulatory focus, and happiness. Results: Mediation analyses demonstrate that TSC positively predicts happiness, while this effect was partially mediated by relatively more promotion focus and less prevention focus. Conclusion: Such findings suggest that people with higher TSC are happier possibly because they are: (1 more promotion-focused on acquiring positive gains thereby facilitating more approach-oriented behaviours, and (2 less prevention-focused on avoiding losses thereby reducing avoidance-oriented behaviours. These findings are relevant for topical scientific debates regarding the underlying mechanisms of self-control regarding initiatory and inhibitory behaviours.

  19. Biochemical Tolerance During Low Dose Propylene Glycol Exposure in Neonates: A Formulation-Controlled Evaluation

    Directory of Open Access Journals (Sweden)

    Aida Kulo

    2012-07-01

    Full Text Available Background and purpose of the study: Propylene glycol (PG is a frequently co-administered solvent in formulations administered to neonates, but reports on its (intolerance are limited. We aimed to report on renal, metabolic and hepatic tolerance before, during and following intravenous (iv PG-paracetamol exposure and compared these data with similar datasets reported in literature on neonates exposed to PG without paracetamol or paracetamol without PG.Methods: Renal (diuresis, creatinemia, sodium, metabolic (Base Excess, Anion Gap, lactate, bicarbonate and hepatic (liver enzymes, bilirubinemia indicators before, during and following iv paracetamol-PG exposure in neonates as included in the PARANEO (paracetamol in neonates study (intra-individual trends, ANOVA were collected and analysed.Comparison with observations collected in cases exposed to either iv phenobarbital-PG or iv paracetamol-mannitol (inter-individual comparison, Mann Whitney-U test were made. Results: PG exposure (median 34.1 mg/kg/24 h did not affect postnatal renal, metabolic and hepatic adaptations in 60 cases exposed to paracetamol-PG. These indicators were similar when compared to 29 cases exposed to phenobarbital- PG or 172 cases exposed to paracetamol-mannitol.Major conclusion: Based on observations in 89 neonates, low dose PG exposure was tolerated well. Studies on PG pharmacokinetics and its covariates are needed to estimate the upper level of PG tolerance in neonates.

  20. Metatranscriptomic insights on gene expression and regulatory controls in Candidatus Accumulibacter phosphatis

    Science.gov (United States)

    Oyserman, Ben O; Noguera, Daniel R; del Rio, Tijana Glavina; Tringe, Susannah G; McMahon, Katherine D

    2016-01-01

    Previous studies on enhanced biological phosphorus removal (EBPR) have focused on reconstructing genomic blueprints for the model polyphosphate-accumulating organism Candidatus Accumulibacter phosphatis. Here, a time series metatranscriptome generated from enrichment cultures of Accumulibacter was used to gain insight into anerobic/aerobic metabolism and regulatory mechanisms within an EBPR cycle. Co-expressed gene clusters were identified displaying ecologically relevant trends consistent with batch cycle phases. Transcripts displaying increased abundance during anerobic acetate contact were functionally enriched in energy production and conversion, including upregulation of both cytoplasmic and membrane-bound hydrogenases demonstrating the importance of transcriptional regulation to manage energy and electron flux during anerobic acetate contact. We hypothesized and demonstrated hydrogen production after anerobic acetate contact, a previously unknown strategy for Accumulibacter to maintain redox balance. Genes involved in anerobic glycine utilization were identified and phosphorus release after anerobic glycine contact demonstrated, suggesting that Accumulibacter routes diverse carbon sources to acetyl-CoA formation via previously unrecognized pathways. A comparative genomics analysis of sequences upstream of co-expressed genes identified two statistically significant putative regulatory motifs. One palindromic motif was identified upstream of genes involved in PHA synthesis and acetate activation and is hypothesized to be a phaR binding site, hence representing a hypothetical PHA modulon. A second motif was identified ~35 base pairs (bp) upstream of a large and diverse array of genes and hence may represent a sigma factor binding site. This analysis provides a basis and framework for further investigations into Accumulibacter metabolism and the reconstruction of regulatory networks in uncultured organisms. PMID:26555245

  1. Control of the B cell-intrinsic tolerance programs by ubiquitin ligases Cbl and Cbl-b.

    Science.gov (United States)

    Kitaura, Yasuyuki; Jang, Ihn Kyung; Wang, Yan; Han, Yoon-Chi; Inazu, Tetsuya; Cadera, Emily J; Schlissel, Mark; Hardy, Richard R; Gu, Hua

    2007-05-01

    B cell receptor (BCR) signaling plays a critical role in B cell tolerance and activation. Here, we show that mice with B cell-specific ablation of both Cbl and Cbl-b (Cbl-/-Cblb-/-) manifested systemic lupus erythematosus (SLE)-like autoimmune disease. The Cbl double deficiency resulted in a substantial increase in marginal zone (MZ) and B1 B cells. The mutant B cells were not hyperresponsive in terms of proliferation and antibody production upon BCR stimulation; however, B cell anergy to protein antigen appeared to be impaired. Concomitantly, BCR-proximal signaling, including tyrosine phosphorylation of Syk tyrosine kinase, Phospholipase C-gamma2 (PLC-gamma2), and Rho-family GTP-GDP exchange factor Vav, and Ca2+ mobilization were enhanced, whereas tyrosine phosphorylation of adaptor protein BLNK was substantially attenuated in the mutant B cells. These results suggested that the loss of coordination between these pathways was responsible for the impaired B cell tolerance induction. Thus, Cbl proteins control B cell-intrinsic checkpoint of immune tolerance, possibly through coordinating multiple BCR-proximal signaling pathways during anergy induction.

  2. Control of the B Cell-Intrinsic Tolerance Program by c-Cbl and Cbl-b

    Science.gov (United States)

    Kitaura, Yasuyuki; Jang, Ihn-Kyung; Wang, Yan; Han, Yoon-Chi; Inazu, Tetsuya; Cadera, Emily J.; Schlissel, Mark; Hardy, Richard R.; Gu, Hua

    2007-01-01

    BCR signaling plays a critical role in B-cell tolerance and activation. Here, we show that mice with B cell-specific ablation of both c-Cbl and Cbl-b (Cbl-dko) manifest systemic lupus erythymatosis (SLE)-like autoimmune disease. The Cbl-dko mutation results in a significant increase in marginal zone (MZ) and B1 B cells. The mutant B cells are not hyperresponsive in terms of proliferation and antibody production upon BCR stimulation; however, B-cell anergy to protein antigen appears to be impaired. Concomitantly, BCR-proximal signaling, including tyrosine phosphorylation of Syk, PLCγ-2, and Vav and Ca2+ mobilization, are enhanced, whereas tyrosine phosphorylation of BLNK is significantly attenuated in the mutant B cells, suggesting that the loss of coordination between these pathways is responsible for the impaired B-cell tolerance induction. Thus, Cbl proteins control B cell-intrinsic checkpoint of immune tolerance, possibly through coordinating multiple BCR-proximal signaling pathways during anergy induction. PMID:17493844

  3. Fault Tolerant Operation of ISOP Multicell Dc-Dc Converter Using Active Gate Controlled SiC Protection Switch

    Directory of Open Access Journals (Sweden)

    Yusuke Hayashi

    2016-01-01

    Full Text Available An active gate controlled semiconductor protection switch using SiC-MOSFET is proposed to achieve the fault tolerant operation of ISOP (Input Series and Output Parallel connected multicell dc-dc converter. The SiC-MOSFET with high temperature capability simplifies the configuration of the protection circuit, and its on-resistance control by the active gate controller realizes the smooth protection without the voltage and the current surges. The first laboratory prototype of the protection switch is fabricated by using a SiC-MOSFET with a high frequency buck chopper for the active gate controller. The effectiveness of the proposed protection switch is verified, taking the impact of the volume reduction into account.

  4. Helicobacter pylori activates the TLR2/NLRP3/caspase-1/IL-18 axis to induce regulatory T-cells, establish persistent infection and promote tolerance to allergens.

    Science.gov (United States)

    Koch, Katrin N; Müller, Anne

    2015-01-01

    The Gram-negative bacterium Helicobacter pylori is both a normal constituent of the human gastric microbiota as well as a pathogen tightly associated with severe gastric disorders. The ability of H. pylori to activate the inflammasome and caspase-1 in antigen-presenting and other cells, and the resulting processing and release of caspase-1-dependent cytokines, impacts both the immunomodulatory and pathogenic activities of H. pylori. This article summarizes recent insights by us and others on the bacterial and host prerequisites of inflammasome activation. H. pylori predominantly activates the NLRP3 inflammasome through a process that requires TLR2-dependent licensing. We identified the urease enzyme, a colonization determinant known to be required for acid adaptation, as critically required for activation of the TLR2/NLRP3/caspase-1 axis. The phenotypes of urease mutants, as well as mouse strains defective for TLR2 or NLRP3, are discussed with respect to their ability to support persistent colonization, immune tolerance and immunity to H. pylori.

  5. Foxp3+ regulatory T cells control persistence of viral CNS infection.

    Directory of Open Access Journals (Sweden)

    Dajana Reuter

    Full Text Available We earlier established a model of a persistent viral CNS infection using two week old immunologically normal (genetically unmodified mice and recombinant measles virus (MV. Using this model infection we investigated the role of regulatory T cells (Tregs as regulators of the immune response in the brain, and assessed whether the persistent CNS infection can be modulated by manipulation of Tregs in the periphery. CD4(+ CD25(+ Foxp3(+ Tregs were expanded or depleted during the persistent phase of the CNS infection, and the consequences for the virus-specific immune response and the extent of persistent infection were analyzed. Virus-specific CD8(+ T cells predominantly recognising the H-2D(b-presented viral hemagglutinin epitope MV-H(22-30 (RIVINREHL were quantified in the brain by pentamer staining. Expansion of Tregs after intraperitoneal (i.p. application of the superagonistic anti-CD28 antibody D665 inducing transient immunosuppression caused increased virus replication and spread in the CNS. In contrast, depletion of Tregs using diphtheria toxin (DT in DEREG (depletion of regulatory T cells-mice induced an increase of virus-specific CD8(+ effector T cells in the brain and caused a reduction of the persistent infection. These data indicate that manipulation of Tregs in the periphery can be utilized to regulate virus persistence in the CNS.

  6. An fMRI Study of Self-Regulatory Control and Conflict Resolution in Adolescents With Bulimia Nervosa

    Science.gov (United States)

    Marsh, Rachel; Horga, Guillermo; Wang, Zhishun; Wang, Pengwei; Klahr, Kristin W.; Berner, Laura A.; Walsh, B. Timothy; Peterson, Bradley S.

    2012-01-01

    Objective The authors examined functional activity in the frontostriatal systems that mediate self-regulatory capacities and conflict resolution in adolescents with bulimia nervosa. Method Functional magnetic resonance imaging was used to compare blood-oxygen-level-dependent response in 18 female adolescents with bulimia nervosa and 18 healthy female age-matched subjects during performance on a Simon spatial incompatibility task. Bayesian analyses were used to compare the two groups on patterns of brain activation during correct responses to conflict stimuli and to explore the effects of antecedent stimulus context on group differences in self-regulation and conflict resolution. Results Adolescents with and without bulimia nervosa performed similarly on the task. During correct responses in conflict trials, frontostriatal circuits—including the right inferolateral and dorsolateral prefrontal cortices and putamen—failed to activate to the same degree in adolescents with bulimia nervosa as in healthy comparison subjects. Instead, deactivation was seen in the left inferior frontal gyrus as well as a neural system encompassing the posterior cingulate cortex and superior frontal gyrus. Group differences in cortical and striatal regions were driven by the differential responses to stimuli preceded by conflict and nonconflict stimuli, respectively. Conclusions When engaging the self-regulatory control processes necessary to resolve conflict, adolescents with bulimia nervosa displayed abnormal patterns of activation in frontostriatal and default-mode systems. Their abnormal processing of the antecedent stimulus context conditioned their brain response to conflict differently from that of healthy comparison subjects, specifically in frontal regions. It is suspected that functional disturbances in frontal portions of frontostriatal systems may release feeding behaviors from regulatory control, thereby perpetuating the conflicting desires to consume fattening foods and

  7. Optimal Heater Control with Technology of Fault Tolerance for Compensating Thermoforming Preheating System

    Directory of Open Access Journals (Sweden)

    Zhen-Zhe Li

    2015-01-01

    Full Text Available The adjustment of heater power is very important because the distribution of thickness strongly depends on the distribution of sheet temperature. In this paper, the steady state optimum distribution of heater power is searched by numerical optimization in order to get uniform sheet temperature. In the following step, optimal heater power distribution with a damaged heater was found out using the technology of fault tolerance, which will be used to reduce the repairing time when some heaters are damaged. The merit of this work is that the design variable was the power of each heater which can be directly used in the preheating process of thermoforming.

  8. SIFT - A preliminary evaluation. [Software Implemented Fault Tolerant computer for aircraft control

    Science.gov (United States)

    Palumbo, D. L.; Butler, R. W.

    1983-01-01

    This paper presents the results of a performance evaluation of the SIFT computer system conducted in the NASA AIRLAB facility. The essential system functions are described and compared to both earlier design proposals and subsequent design improvements. The functions supporting fault tolerance are found to consume significant computing resources. With SIFT's specimen task load, scheduled at a 30-Hz rate, the executive tasks such as reconfiguration, clock synchronization and interactive consistency, require 55 percent of the available task slots. Other system overhead (e.g., voting and scheduling) use an average of 50 percent of each remaining task slot.

  9. Regulatory T cell memory

    Science.gov (United States)

    Rosenblum, Michael D.; Way, Sing Sing; Abbas, Abul K.

    2016-01-01

    Memory for antigen is a defining feature of adaptive immunity. Antigen-specific lymphocyte populations show an increase in number and function after antigen encounter and more rapidly re-expand upon subsequent antigen exposure. Studies of immune memory have primarily focused on effector B cells and T cells with microbial specificity, using prime challenge models of infection. However, recent work has also identified persistently expanded populations of antigen-specific regulatory T cells that protect against aberrant immune responses. In this Review, we consider the parallels between memory effector T cells and memory regulatory T cells, along with the functional implications of regulatory memory in autoimmunity, antimicrobial host defence and maternal fetal tolerance. In addition, we discuss emerging evidence for regulatory T cell memory in humans and key unanswered questions in this rapidly evolving field. PMID:26688349

  10. Regulatory agencies and regulatory risk

    OpenAIRE

    Knieps, Günter; Weiß, Hans-Jörg

    2007-01-01

    The aim of this paper is to show that regulatory risk is due to the discretionary behaviour of regulatory agencies, caused by a too extensive regulatory mandate provided by the legislator. The normative point of reference and a behavioural model of regulatory agencies based on the positive theory of regulation are presented. Regulatory risk with regard to the future behaviour of regulatory agencies is modelled as the consequence of the ex ante uncertainty about the relative influence of inter...

  11. Stable Fault-tolerance Control for a Class of Networked Control Systems%一类网络化控制系统的稳定容错控制

    Institute of Scientific and Technical Information of China (English)

    孔德明; 方华京

    2005-01-01

    In this paper, we use the matrix measure technique to study stable fault-tolerance control of networked control systems. State feedback networked control systems with the network-induced delay, parameter uncertainties, sensor failures and actuator failures are considered. State feedback gain K is designed for any invariant delay τ, and some theorems and sufficient conditions for stable fault-tolerance control are given. Example is presented to illustrate the effectiveness of these theorems.

  12. Characterization of a putative cis-regulatory element that controls transcriptional activity of the pig uroplakin II gene promoter.

    Science.gov (United States)

    Kwon, Deug-Nam; Park, Mi-Ryung; Park, Jong-Yi; Cho, Ssang-Goo; Park, Chankyu; Oh, Jae-Wook; Song, Hyuk; Kim, Jae-Hwan; Kim, Jin-Hoi

    2011-07-01

    Uroplakin II (UPII) is a one of the integral membrane proteins synthesized as a major differentiation product of mammalian urothelium. UPII gene expression is bladder specific and differentiation dependent, but little is known about its transcription response elements and molecular mechanism. To identify the cis-regulatory elements in the pig UPII (pUPII) gene promoter region, we constructed pUPII 5' upstream region deletion mutants and demonstrated that each of the deletion mutants participates in controlling the expression of the pUPII gene in human bladder carcinoma RT4 cells. We also identified a new core promoter region and putative negative cis-regulatory element within a minimal promoter region. In addition, we showed that hepatocyte nuclear factor 4 (HNF4) can directly bind in the pUPII core promoter (5F-1) region, which plays a critical role in controlling promoter activity. Transient cotransfection experiments showed that HNF4 positively regulates pUPII gene promoter activity. Thus, the binding element and its binding protein, HNF4 transcription factor, may be involved in the mechanism that specifically regulates pUPII gene transcription.

  13. Microbiological water methods: quality control measures for Federal Clean Water Act and Safe Drinking Water Act regulatory compliance.

    Science.gov (United States)

    Root, Patsy; Hunt, Margo; Fjeld, Karla; Kundrat, Laurie

    2014-01-01

    Quality assurance (QA) and quality control (QC) data are required in order to have confidence in the results from analytical tests and the equipment used to produce those results. Some AOAC water methods include specific QA/QC procedures, frequencies, and acceptance criteria, but these are considered to be the minimum controls needed to perform a microbiological method successfully. Some regulatory programs, such as those at Code of Federal Regulations (CFR), Title 40, Part 136.7 for chemistry methods, require additional QA/QC measures beyond those listed in the method, which can also apply to microbiological methods. Essential QA/QC measures include sterility checks, reagent specificity and sensitivity checks, assessment of each analyst's capabilities, analysis of blind check samples, and evaluation of the presence of laboratory contamination and instrument calibration and checks. The details of these procedures, their performance frequency, and expected results are set out in this report as they apply to microbiological methods. The specific regulatory requirements of CFR Title 40 Part 136.7 for the Clean Water Act, the laboratory certification requirements of CFR Title 40 Part 141 for the Safe Drinking Water Act, and the International Organization for Standardization 17025 accreditation requirements under The NELAC Institute are also discussed.

  14. A Soft Sensor-Based Fault-Tolerant Control on the Air Fuel Ratio of Spark-Ignition Engines

    Directory of Open Access Journals (Sweden)

    Yu-Jia Zhai

    2017-01-01

    Full Text Available The air/fuel ratio (AFR regulation for spark-ignition (SI engines has been an essential and challenging control problem for engineers in the automotive industry. The feed-forward and feedback scheme has been investigated in both academic research and industrial application. The aging effect can often cause an AFR sensor fault in the feedback loop, and the AFR control performance will degrade consequently. In this research, a new control scheme on AFR with fault-tolerance is proposed by using an artificial neural network model based on fault detection and compensation, which can provide the satisfactory AFR regulation performance at the stoichiometric value for the combustion process, given a certain level of misreading of the AFR sensor.

  15. The Ets-1 transcription factor controls the development and function of natural regulatory T cells

    Science.gov (United States)

    Mouly, Enguerran; Chemin, Karine; Nguyen, Hai Vu; Chopin, Martine; Mesnard, Laurent; Leite-de-Moraes, Maria; Burlen-defranoux, Odile; Bandeira, Antonio

    2010-01-01

    Regulatory T cells (T reg cells) constitute a population of CD4+ T cells that limits immune responses. The transcription factor Foxp3 is important for determining the development and function of T reg cells; however, the molecular mechanisms that trigger and maintain its expression remain incompletely understood. In this study, we show that mice deficient for the Ets-1 transcription factor (Ets-1−/−) developed T cell–mediated splenomegaly and systemic autoimmunity that can be blocked by functional wild-type T reg cells. Spleens of Ets-1−/− mice contained mostly activated T cells, including Th2-polarized CD4+ cells and had reduced percentages of T reg cells. Splenic and thymic Ets-1−/− T reg cells expressed low levels of Foxp3 and displayed the CD103 marker that characterizes antigen-experienced T reg cells. Thymic development of Ets-1−/− T reg cells appeared intrinsically altered as Foxp3-expressing cells differentiate poorly in mixed fetal liver reconstituted chimera and fetal thymic organ culture. Ets-1−/− T reg cells showed decreased in vitro suppression activity and did not protect Rag2−/− hosts from naive T cell–induced inflammatory bowel disease. Furthermore, in T reg cells, Ets-1 interacted with the Foxp3 intronic enhancer and was required for demethylation of this regulatory sequence. These data demonstrate that Ets-1 is required for the development of natural T reg cells and suggest a role for this transcription factor in the regulation of Foxp3 expression. PMID:20855499

  16. Prostaglandin E2 potentiates mesenchymal stem cell-induced IL-10+IFN-γ+CD4+ regulatory T cells to control transplant arteriosclerosis.

    Science.gov (United States)

    Hsu, Wan-Tseng; Lin, Cheng-Hsin; Chiang, Bor-Luen; Jui, Hsiang-Yiang; Wu, Kenneth Kun-Yu; Lee, Chii-Ming

    2013-03-01

    Mesenchymal stem cells (MSCs) are known for their immunomodulatory functions. We previously demonstrated that bone marrow-derived MSCs effectively control transplant arteriosclerosis (TA) by enhancing IL-10(+) and IFN-γ(+) cells. The objective of this study is to elucidate the mechanism by which MSCs induce IL-10(+)IFN-γ(+)CD4(+) regulatory T type 1 (T(R)1)-like cells. In an MLR system using porcine PBMCs, MSC-induced IL-10(+)IFN-γ(+)CD4(+) cells, which confer resistance to allogeneic proliferation in an IL-10-dependent manner, resemble T(R)1-like cells. Both cyclooxygenase-derived PGE(2) and IDO help to induce T(R)1-like cells by MSCs. MSCs constitutively secrete PGE(2), which is augmented in allogeneic reactions. However, T(R)1-like cells were deficient in PGE(2) and 4-fold less potent than were MSCs in suppressing MLR. PGE(2) mimetic supplements can enhance the immunosuppressive potency of T(R)1-like cells. In a porcine model of allogeneic femoral arterial transplantation, MSC-induced T(R)1-like cells combined with PGE(2), but not either alone, significantly reduced TA at the end of 6 wk (percentage of luminal area stenosis: T(R)1-like cells + PGE(2): 11 ± 10%; PGE(2) alone: 93 ± 8.7%; T(R)1-like cells alone: 88 ± 2.4% versus untreated 94 ± 0.9%, p < 0.001). These findings indicate that PGE(2) helps MSC-induced IL-10(+)IFN-γ(+)CD4(+) T(R)1-like cells inhibit TA. PGE(2) combined with MSC-induced T(R)1-like cells represents a new approach for achieving immune tolerance.

  17. The development of basic technology for instrumentation and control - A study on the development of fault-tolerant digital control systems

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Sang Jeong; Seong, Se Jin; Yoon, Sang Joon; Park, Sang Hyun; Jeong, Il Young; Kwon, Hyun Jin [Chungnam National University, Taejon (Korea, Republic of); Kim, Sang Woo [Pohang University of Science and Technology, Pohang (Korea, Republic of)

    1996-07-01

    This project developed fault-tolerant control algorithm with bumpless transfer applicable to the steam generator level control system in the low power operating range. A bumpless transfer scheme is introduced for removing the bump phenomenon in the control signal when control failures occur. In particular, the bumpless scheme can be efficiently used with the proposed PID= and AGPC control algorithm has shown good performance in the simulation study. This project also analyzed V and V system structure and summarized PID tuning methods for porting the proposed algorithms to V and V system. Finally, this project developed a test bed where PID and AGPC algorithm is implemented and the derived steam generator model is included as the plant. 51 refs., 17 tabs., 88 figs. (author)

  18. RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking.

    Science.gov (United States)

    Orozco, Helena; Sepúlveda, Ana; Picazo, Cecilia; Matallana, Emilia; Aranda, Agustín

    2016-06-01

    Glycerol is a key yeast metabolite in winemaking because it contributes to improve the organoleptic properties of wine. It is also a cellular protective molecule that enhances the tolerance of yeasts to osmotic stress and promotes longevity. Thus, its production increases by genetic manipulation, which is of biotechnological and basic interest. Glycerol is produced by diverting glycolytic glyceraldehyde-3-phosphate through the action of glycerol-3-phosphate dehydrogenase (coded by genes GPD1 and GPD2). Here, we demonstrate that RNA-binding protein Pub1p regulates glycerol production by controlling Gpd1p activity. Its deletion does not alter GPD1 mRNA levels, but protein levels and enzymatic activity increase, which explains the higher intracellular glycerol concentration and greater tolerance to osmotic stress of the pub1∆ mutant. PUB1 deletion also enhances the activity of nicotinamidase, a longevity-promoting enzyme. Both enzymatic activities are partially located in peroxisomes, and we detected peroxisome formation during wine fermentation. The role of Pub1p in life span control depends on nutrient conditions and is related with the TOR pathway, and a major connection between RNA metabolism and the nutrient signaling response is established.

  19. Tolerance of freshwater test organisms to formulated sediments for use as control materials in whole-sediment toxicity tests

    Energy Technology Data Exchange (ETDEWEB)

    Kemble, N.E.; Dwyer, F.J.; Ingersoll, C.G. [Geological Survey, Columbia, MO (United States). Environmental and Contaminants Research Center; Dawson, T.D. [Integrated Laboratory Systems, Duluth, MN (United States); Norberg-King, T.J. [Environmental Protection Agency, Duluth, MN (United States). Mid-Continent Ecological Div.

    1999-02-01

    A method is described for preparing formulated sediments for use in toxicity testing. Ingredients used to prepare formulated sediments included commercially available silt, clay, sand, humic acid, dolomite, and {alpha}-cellulose (as a source of organic carbon). {alpha}-Cellulose was selected as the source of organic carbon because it is commercially available, consistent from batch to batch, and low in contaminant concentrations. The tolerance of freshwater test organisms to formulated sediments for use as control materials in whole-sediment toxicity testing was evaluated. Sediment exposures were conducted for 10 d with the amphipod Hyalella azteca, the midges Chironomus riparius and C. tentans, and the oligochaete Lumbriculus variegatus and for 28 d with H. azteca. Responses of organisms in formulated sediments was compared with a field-collected control sediment that has routinely been used to determine test acceptability. Tolerance of organisms to formulated sediments was evaluated by determining responses to varying levels of {alpha}-cellulose, to varying levels of grain size, to evaluation of different food types, or to evaluation of different sources of overlying water. In the 10-d exposures, survival of organisms exposed to the formulated sediments routinely met or exceeded the responses of test organisms exposed to the control sediment and routinely met test acceptability criteria required in standard methods. Growth of amphipods and oligochaetes in 10-d exposures with formulated sediment was often less than growth of organisms in the field-collected control sediment. Additional research is needed, using the method employed to prepare formulated sediment, to determine if conditioning formulated sediments before starting 10-d tests would improve the growth of amphipods. In the 28-d exposures, survival of H. azteca was low when reconstituted water was used as the source of overlying water. However, when well water was used as the source of overlying water in

  20. Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages

    Institute of Scientific and Technical Information of China (English)

    LIU Fengxia; SUN Chuanqing; TAN Lubin; FU Yongcai; LI Dejun; WANG Xiangkun

    2003-01-01

    An advanced backcross population of rice was used to identify the quantitative trait locus (QTL) controlling the cold-tolerance at booting to flowering stages. The recipient, Guichao 2 (GC2), was a commercial Indica rice; the donor Dongxiang common wild rice, was an accession of common wild rice (DXCWR, Oryza rufipogon Griff.). Three QTLs for cold-tolerance were detected on chromosomes 1, 6and 11. Two of them coming from DXCWR could enhance the cold-tolerance of the backcross progenies. Moreover, one sterility QTL that could reduce the seed set rate of the backcross progenies by 78% was mapped on chromosome 5.

  1. Understanding genetic control of drought tolerance through meta-QTL analysis for genomics-assisted improvement of drought tolerance in peanut (Arachis hypogaeaL.)

    Science.gov (United States)

    Majority of peanut is cultivated in the semi-arid tropical regions of the world where abiotic stresses especially drought is a major constraint for crop production. With an objective of undertaking molecular breeding approaches for developing superior lines with enhanced drought tolerance, several Q...

  2. The Radiation Tolerant Electronics for the LHC Cryogenic Controls: Basic Design and First Operational Experience

    CERN Document Server

    Casas-Cubillos, J; Rodríguez-Ruiz, M A

    2008-01-01

    The LHC optics is based in the extensive use of superconducting magnets covering 23 km inside the tunnel. The associated cryogenic system for keeping the magnets in nominal conditions is hence distributed all around the 27 km LHC tunnel and the cryogenic instrumentation submitted to the LHC radiation environment is composed of about 18’000 sensors and actuators. Radiation Tolerant (RadTol) electronics was designed and procured in order to keep the signals integrity against electromagnetic interference and to reduce cabling costs required in case of sending the analog signals into the 30 radiation protected areas. This paper presents the basic design, the qualification of the main RadTol components and the first operational results.

  3. A fault tolerant design for autonomous attitude control of the DSCS-III communication satellite

    Science.gov (United States)

    Matijevic, J.; Mettler, E.

    1983-01-01

    The first of a new series of satellites, which will provide the principal elements in the Defense Space Communications System (DSCS), was launched on Oct. 31, 1982. This satellite, DSCS-III, is part of a system which will consist of super-high frequency communications satellites in synchronous, equatorial orbits, continuously operating in four widely separate geographic regions. The DSCS-III is designed both to maintain critical communications in the presence of an electronic jamming threat and to survive nuclear radiation exposure. The results of the present investigation are to provide a basis for the design of a spacecraft tolerant of on-board failures, survivable against external threats, and capable of performing its mission autonomously for periods as long as six months.

  4. Design and RAMS Analysis of a Fault-Tolerant Computer Control System

    Institute of Scientific and Technical Information of China (English)

    WANG Shuai; JI Yindong; DONG Wei; YANG Shiyuan

    2007-01-01

    This paper presents a fault-tolerant computer system. It is designed as a double 2-out-of-2 architecture based on component redundant technique. Also, a quantitative probabilistic model is presented for evaluating the reliability, availability, maintainability and safety (RAMS) of this architecture. Hierarchical modeling method and Markov modeling method are used in RAMS analysis to evaluate the system characteristics. The double 2-out-of-2 system is compared with the other two systems, all voting triple modular redundancy (AVTMR) system and dual-duplex system. According to the result, the double 2-out-of-2 system has the highest dependability. Especially, the system can satisfy the safety integrity level (SIL) 4, which means the system's probability of catastrophic failure less than or equal to 10~8 per hour, therefore, it can be applied to life critical systems such as high-speed railway systems.

  5. USAGE OF STANDARD PERSONAL COMPUTER PORTS FOR DESIGNING OF THE DOUBLE REDUNDANT FAULT-TOLERANT COMPUTER CONTROL SYSTEMS

    Directory of Open Access Journals (Sweden)

    Rafig SAMEDOV

    2005-01-01

    Full Text Available In this study, for designing of the fault-tolerant control systems by using standard personal computers, the ports have been investigated, different structure versions have been designed and the method for choosing of an optimal structure has been suggested. In this scope, first of all, the ÇİFTYAK system has been defined and its work principle has been determined. Then, data transmission ports of the standard personal computers have been classified and analyzed. After that, the structure versions have been designed and evaluated according to the used data transmission methods, the numbers of ports and the criterions of reliability, performance, truth, control and cost. Finally, the method for choosing of the most optimal structure version has been suggested.

  6. Fault Diagnosis and Fault-tolerant Control of Modular Multi-level Converter High-voltage DC System: A Review

    DEFF Research Database (Denmark)

    Liu, Hui; Ma, Ke; Wang, Chao;

    2016-01-01

    Modular Multilevel Converter based High Voltage Direct Current (MMC-HVDC) configuration is a promising solution for the efficient grid integration and bulky power transmission over long distance. However, the large number of series connected identical modules in MMC may increase the probability...... strategies of MMC-HVDC systems for the most common faults happened in MMC-HVDC systems covering MMC faults, DC side faults as well as AC side faults. An important part of this paper is devoted to a discussion of the vulnerable spots as well as failure mechanism of the MMC-HVDC system covering switching...... device fault, DC line faults as well as AC grid faults. Special attention is given to the comparison of the corresponding fault diagnosis and fault-tolerant control approaches. Further, focus is dedicated to control/protection strategies and topologies with fault ride-though capability for MMC...

  7. Reiterative AP2a activity controls sequential steps in the neural crest gene regulatory network.

    Science.gov (United States)

    de Crozé, Noémie; Maczkowiak, Frédérique; Monsoro-Burq, Anne H

    2011-01-04

    The neural crest (NC) emerges from combinatorial inductive events occurring within its progenitor domain, the neural border (NB). Several transcription factors act early at the NB, but the initiating molecular events remain elusive. Recent data from basal vertebrates suggest that ap2 might have been critical for NC emergence; however, the role of AP2 factors at the NB remains unclear. We show here that AP2a initiates NB patterning and is sufficient to elicit a NB-like pattern in neuralized ectoderm. In contrast, the other early regulators do not participate in ap2a initiation at the NB, but cooperate to further establish a robust NB pattern. The NC regulatory network uses a multistep cascade of secreted inducers and transcription factors, first at the NB and then within the NC progenitors. Here we report that AP2a acts at two distinct steps of this cascade. As the earliest known NB specifier, AP2a mediates Wnt signals to initiate the NB and activate pax3; as a NC specifier, AP2a regulates further NC development independent of and downstream of NB patterning. Our findings reconcile conflicting observations from various vertebrate organisms. AP2a provides a paradigm for the reiterated use of multifunctional molecules, thereby facilitating emergence of the NC in vertebrates.

  8. Regulatory T cells control strain specific resistance to Experimental Autoimmune Prostatitis.

    Science.gov (United States)

    Breser, Maria L; Lino, Andreia C; Motrich, Ruben D; Godoy, Gloria J; Demengeot, Jocelyne; Rivero, Virginia E

    2016-09-14

    Susceptibility to autoimmune diseases results from the encounter of a complex and long evolved genetic context with a no less complex and changing environment. Major actors in maintaining health are regulatory T cells (Treg) that primarily dampen a large subset of autoreactive lymphocytes escaping thymic negative selection. Here, we directly asked whether Treg participate in defining susceptibility and resistance to Experimental Autoimmune Prostatitis (EAP). We analyzed three common laboratory strains of mice presenting with different susceptibility to autoimmune prostatitis upon immunization with prostate proteins. The NOD, the C57BL/6 and the BALB/c mice that can be classified along a disease score ranging from severe, mild and to undetectable, respectively. Upon mild and transient depletion of Treg at the induction phase of EAP, each model showed an increment along this score, most remarkably with the BALB/c mice switching from a resistant to a susceptible phenotype. We further show that disease associates with the upregulation of CXCR3 expression on effector T cells, a process requiring IFNγ. Together with recent advances on environmental factors affecting Treg, these findings provide a likely cellular and molecular explanation to the recent rise in autoimmune diseases incidence.

  9. Regulatory Bodies: Dramatic Creativity, Control and the Commodity of Lady Audley's Secret

    Directory of Open Access Journals (Sweden)

    Kate Mattacks

    2009-04-01

    Full Text Available 'Lady Audley's Secret' resonates with a sense of theatricality grounded in the eponymous heroine's propensity for visual tactics to attract an audience. Repositioning the dramatic exchange between actress and audience within the domestic sphere sensitised the reader to the question of diagnosing morally degenerative artificiality whilst involving the reader in the complicit act of encouraging Lady Audley's performance through consumer demand. This complex relationship between consumerism, theatre and society saw Lady Audley's figure assume an iconic status, but little has been written on the creative interplay between the novel and the theatre itself. Beginning with the way in which aspiring actresses used Lady Audley as a model to display their histrionic variety, I argue that the theatre used her character to engage with the complex connections between drama and commodity culture. Her autophagy in favour of a false identity provided a model for actresses to visualise self-commodification whilst highlighting the tension between the material practices of the theatre and the materiality of commodity culture. The drive for regulation of the theatrical medium finds its locus in the licensing text/published acting edition of William E. Suter's 'Lady Audley's Secret' (1863. Here I move to show how Suter's adaptation functioned as a site where the state regulation of the theatre was explored and contested. These unauthorised images of Lady Audley's containment came to symbolise not only the regulatory processes of licensing and copyright, but also the creative exploitation of a commodity culture critiqued through the dehumanised form of Braddon's original heroine.

  10. MicroRNAs in control of gene regulatory programs in diabetic vasculopathy.

    Science.gov (United States)

    Pei, Chongzhe; Zhang, Xiaoping; Meng, Shu; Li, Yigang

    2017-01-01

    Diabetes is generally associated with vasculopathy, which contains both microvascular and macrovascular complications, associated with high morbidity and mortality. Currently, despite interventional therapy, the overall prognosis for patients with diabetic vasculopathy remains unsatisfactory. Angiogenesis and vascular injury and repair are associated with a variety of cells. However, the molecular mechanisms of the cells that are involved in pathogenesis of diabetic vasculopathy remain largely unknown. As novel molecules, microRNAs (miRs) take part in regulating protein-coding gene expression at the post-transcriptional level, and contribute to the pathogenesis of various types of chronic metabolism disease, especially diabetic vasculopathy. This allows miRs to have a direct function in regulation of various cellular events. Additionally, circulating miRs have been proposed as biomarkers for a wide range of cardiovascular diseases. This review elucidates miR-mediated regulatory mechanisms in diabetic vasculopathy. Furthermore, we discuss the current understanding of miRs in diabetic vasculopathy. Finally, we summarize the development of novel diagnostic and therapeutic strategies for diabetic vasculopathy related to miRs.

  11. A kissing loop is important for btuB riboswitch ligand sensing and regulatory control.

    Science.gov (United States)

    Lussier, Antony; Bastet, Laurène; Chauvier, Adrien; Lafontaine, Daniel A

    2015-10-30

    RNA-based genetic regulation is exemplified by metabolite-binding riboswitches that modulate gene expression through conformational changes. Crystal structures show that the Escherichia coli btuB riboswitch contains a kissing loop interaction that is in close proximity to the bound ligand. To analyze the role of the kissing loop interaction in the riboswitch regulatory mechanism, we used RNase H cleavage assays to probe the structure of nascent riboswitch transcripts produced by the E. coli RNA polymerase. By monitoring the folding of the aptamer, kissing loop, and riboswitch expression platform, we established the conformation of each structural component in the absence or presence of bound adenosylcobalamin. We found that the kissing loop interaction is not essential for ligand binding. However, we showed that kissing loop formation improves ligand binding efficiency and is required to couple ligand binding to the riboswitch conformational changes involved in regulating gene expression. These results support a mechanism by which the btuB riboswitch modulates the formation of a tertiary structure to perform metabolite sensing and regulate gene expression. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  12. A Kissing Loop Is Important for btuB Riboswitch Ligand Sensing and Regulatory Control*

    Science.gov (United States)

    Lussier, Antony; Bastet, Laurène; Chauvier, Adrien; Lafontaine, Daniel A.

    2015-01-01

    RNA-based genetic regulation is exemplified by metabolite-binding riboswitches that modulate gene expression through conformational changes. Crystal structures show that the Escherichia coli btuB riboswitch contains a kissing loop interaction that is in close proximity to the bound ligand. To analyze the role of the kissing loop interaction in the riboswitch regulatory mechanism, we used RNase H cleavage assays to probe the structure of nascent riboswitch transcripts produced by the E. coli RNA polymerase. By monitoring the folding of the aptamer, kissing loop, and riboswitch expression platform, we established the conformation of each structural component in the absence or presence of bound adenosylcobalamin. We found that the kissing loop interaction is not essential for ligand binding. However, we showed that kissing loop formation improves ligand binding efficiency and is required to couple ligand binding to the riboswitch conformational changes involved in regulating gene expression. These results support a mechanism by which the btuB riboswitch modulates the formation of a tertiary structure to perform metabolite sensing and regulate gene expression. PMID:26370077

  13. An Integrative Approach to Computational Modelling of the Gene Regulatory Network Controlling Clostridium botulinum Type A1 Toxin Production

    Science.gov (United States)

    Walshaw, John; Peck, Michael W.; Barker, Gary C.

    2016-01-01

    Clostridium botulinum produces botulinum neurotoxins (BoNTs), highly potent substances responsible for botulism. Currently, mathematical models of C. botulinum growth and toxigenesis are largely aimed at risk assessment and do not include explicit genetic information beyond group level but integrate many component processes, such as signalling, membrane permeability and metabolic activity. In this paper we present a scheme for modelling neurotoxin production in C. botulinum Group I type A1, based on the integration of diverse information coming from experimental results available in the literature. Experiments show that production of BoNTs depends on the growth-phase and is under the control of positive and negative regulatory elements at the intracellular level. Toxins are released as large protein complexes and are associated with non-toxic components. Here, we systematically review and integrate those regulatory elements previously described in the literature for C. botulinum Group I type A1 into a population dynamics model, to build the very first computational model of toxin production at the molecular level. We conduct a validation of our model against several items of published experimental data for different wild type and mutant strains of C. botulinum Group I type A1. The result of this process underscores the potential of mathematical modelling at the cellular level, as a means of creating opportunities in developing new strategies that could be used to prevent botulism; and potentially contribute to improved methods for the production of toxin that is used for therapeutics. PMID:27855161

  14. A model for the regulatory network controlling the dynamics of kinetochore microtubule plus-ends and poleward flux in metaphase.

    Science.gov (United States)

    Fernandez, Nicolas; Chang, Qiang; Buster, Daniel W; Sharp, David J; Ma, Ao

    2009-05-12

    Tight regulation of kinetochore microtubule dynamics is required to generate the appropriate position and movement of chromosomes on the mitotic spindle. A widely studied but mysterious aspect of this regulation occurs during metaphase when polymerization of kinetochore microtubule plus-ends is balanced by depolymerization at their minus-ends. Thus, kinetochore microtubules maintain a constant net length, allowing chromosomes to persist at the spindle equator, but consist of tubulin subunits that continually flux toward spindle poles. Here, we construct a feasible network of regulatory proteins for controlling kinetochore microtubule plus-end dynamics, which was combined with a Monte Carlo algorithm to simulate metaphase tubulin flux. We also test the network model by combining it with a force-balancing model explicitly taking force generators into account. Our data reveal how relatively simple interrelationships among proteins that stimulate microtubule plus-end polymerization, depolymerization, and dynamicity can induce robust flux while accurately predicting apparently contradictory results of knockdown experiments. The model also provides a simple and robust physical mechanism through which the regulatory networks at kinetochore microtubule plus- and minus-ends could communicate.

  15. A Control Strategy for Single-phase Grid-Connected Inverter with Power Quality Regulatory Function

    Directory of Open Access Journals (Sweden)

    Luo Ling

    2013-07-01

    Full Text Available A single-phase grid-connected inverter system based on LCL filter is established,which combines the features of inverter and active power filter. A composite control strategy for grid-connected inverter with the function of implementing reactive power compensation and harmonic compensation in the grid-connected power generation is proposed. Firstly, grid-connected inverter system structure and model is analyzed. A quasi-Proportional Resonant control method to gain the control of grid-connected fundamental wave current containing reactive power compensation current as well as the control of harmonic compensation currents is put forward; then the calculation methods of  composite control command current based on both second order generalized integrator-quadrature signal generator (SOGI-QSG and instantaneous reactive power theory are given. Finally, the effectiveness of the control strategy proposed in this paper is verified by simulation.

  16. PcFKH1, a novel regulatory factor from the forkhead family, controls the biosynthesis of penicillin in Penicillium chrysogenum.

    Science.gov (United States)

    Domínguez-Santos, Rebeca; García-Estrada, Carlos; Kosalková, Katarina; Prieto, Carlos; Santamarta, Irene; Martín, Juan-Francisco

    2015-08-01

    Penicillin biosynthesis in Penicillium chrysogenum (re-identified as Penicillium rubens) is a good example of a biological process subjected to complex global regulatory networks and serves as a model to study fungal secondary metabolism. The winged-helix family of transcription factors recently described, which includes the forkhead type of proteins, is a key type of regulatory proteins involved in this process. In yeasts and humans, forkhead transcription factors are involved in different processes (cell cycle regulation, cell death control, pre-mRNA processing and morphogenesis); one member of this family of proteins has been identified in the P. chrysogenum genome (Pc18g00430). In this work, we have characterized this novel transcription factor (named PcFKH1) by generating knock-down mutants and overexpression strains. Results clearly indicate that PcFKH1 positively controls antibiotic biosynthesis through the specific interaction with the promoter region of the penDE gene, thus regulating penDE mRNA levels. PcFKH1 also binds to the pcbC promoter, but with low affinity. In addition, it also controls other ancillary genes of the penicillin biosynthetic process, such as phlA (encoding phenylacetyl CoA ligase) and ppt (encoding phosphopantetheinyl transferase). PcFKH1 also plays a role in conidiation and spore pigmentation, but it does not seem to be involved in hyphal morphology or cell division in the improved laboratory reference strain Wisconsin 54-1255. A genome-wide analysis of processes putatively coregulated by PcFKH1 and PcRFX1 (another winged-helix transcription factor) in P. chrysogenum provided evidence of the global effect of these transcription factors in P. chrysogenum metabolism.

  17. Influence of Protoplasmic Water Loss on the Control of Protein Synthesis in the Desiccation-Tolerant Moss Tortula ruralis 1

    Science.gov (United States)

    Oliver, Melvin J.

    1991-01-01

    Desiccation tolerance of the moss Tortula ruralis is characterized by a desiccation-induced change in gene expression that becomes evident upon rehydration. As reported earlier, this change in gene expression is apparently brought about by a change in the control of translation and does not include a major shift in mRNA abundance. A full qualitative and quantitative analysis of the alteration in gene expression, which is characterized by the loss of (or greater than fivefold decrease in) the synthesis of 25 hydration (h) proteins and initiation (or greater than fivefold increase) of the synthesis of 74 rehydration (r) proteins, is given in this report. Exposure to a desiccating atmosphere, for times that result in varying levels of water loss, enabled the determination that the control of synthesis of r proteins is different from the control of synthesis of h proteins. The r and h protein synthesis responses are internally coordinate, however. Similarly, the return to normal levels of h protein synthesis differs from that of the r proteins. The return to normal synthetic levels for all h proteins is synchronous, but the rate of loss of r protein synthesis varies with each individual r protein. Run-off translation of polysomes isolated from gametophytes during the drying phase demonstrates that there are no novel mRNAs recruited and no particular mRNA is favored for translation during desiccation. These findings add credence to the argument that translational control is the major component of the desiccation-induced alteration in gene expression in this plant, as discussed. Aspects of the response of protein synthesis to desiccation are consistent with the hypothesis that T. ruralis exhibits a repair-based mechanism of desiccation tolerance. ImagesFigure 2Figure 3Figure 5Figure 6Figure 7 PMID:16668577

  18. Toll-like receptors on regulatory T cells: expanding immune regulation.

    NARCIS (Netherlands)

    Sutmuller, R.P.M.; Morgan, M.E.; Netea, M.G.; Grauer, O.M.; Adema, G.J.

    2006-01-01

    Regulatory T (Treg) cells maintain peripheral tolerance and limit effector responses to prevent excessive immune-mediated tissue damage. However, recent research reveals that Treg cells also dampen the induction of immune responses and, thus, must be controlled to enable the effective protection aga

  19. Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production

    Directory of Open Access Journals (Sweden)

    Lucas Faustino

    2012-01-01

    Full Text Available Foxp3+CD25+CD4+ regulatory T cells are vital for peripheral tolerance and control of tissue inflammation. In this study, we characterized the phenotype and monitored the migration and activity of regulatory T cells present in the airways of allergic or tolerant mice after allergen challenge. To induce lung allergic inflammation, mice were sensitized twice with ovalbumin/aluminum hydroxide gel and challenged twice with intranasal ovalbumin. Tolerance was induced by oral administration of ovalbumin for 5 consecutive days prior to OVA sensitization and challenge. We detected regulatory T cells (Foxp3+CD25+CD4+ T cells in the airways of allergic and tolerant mice; however, the number of regulatory T cells was more than 40-fold higher in allergic mice than in tolerant mice. Lung regulatory T cells expressed an effector/memory phenotype (CCR4highCD62LlowCD44highCD54highCD69+ that distinguished them from naive regulatory T cells (CCR4intCD62LhighCD44intCD54intCD69−. These regulatory T cells efficiently suppressed pulmonary T-cell proliferation but not Th2 cytokine production.

  20. Identification of Cell Wall Synthesis Regulatory Genes Controlling Biomass Characteristics and Yield in Rice (Oryza Sativa)

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Zhaohua PEng [Mississippi State University; Ronald, Palmela [UC-Davis; Wang, Guo-Liang [The Ohio State University

    2013-04-26

    This project aims to identify the regulatory genes of rice cell wall synthesis pathways using a cell wall removal and regeneration system. We completed the gene expression profiling studies following the time course from cell wall removal to cell wall regeneration in rice suspension cells. We also completed, total proteome, nuclear subproteome and histone modification studies following the course from cell wall removal and cell wall regeneration process. A large number of differentially expressed regulatory genes and proteins were identified. Meanwhile, we generated RNAi and over-expression transgenic rice for 45 genes with at least 10 independent transgenic lines for each gene. In addition, we ordered T-DNA and transposon insertion mutants for 60 genes from Korea, Japan, and France and characterized the mutants. Overall, we have mutants and transgenic lines for over 90 genes, exceeded our proposed goal of generating mutants for 50 genes. Interesting Discoveries a) Cell wall re-synthesis in protoplasts may involve a novel cell wall synthesis mechanism. The synthesis of the primary cell wall is initiated in late cytokinesis with further modification during cell expansion. Phragmoplast plays an essential role in cell wall synthesis. It services as a scaffold for building the cell plate and formation of a new cell wall. Only one phragmoplast and one new cell wall is produced for each dividing cell. When the cell wall was removed enzymatically, we found that cell wall re-synthesis started from multiple locations simultaneously, suggesting that a novel mechanism is involved in cell wall re-synthesis. This observation raised many interesting questions, such as how the starting sites of cell wall synthesis are determined, whether phragmoplast and cell plate like structures are involved in cell wall re-synthesis, and more importantly whether the same set of enzymes and apparatus are used in cell wall re-synthesis as during cytokinesis. Given that many known cell wall