WorldWideScience

Sample records for controls genome stability

  1. Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability

    DEFF Research Database (Denmark)

    Lauritsen, Ida; Martinez, Virginia; Ronda, Carlotta

    2018-01-01

    In molecular biology and cell factory engineering, tools that enable control of protein production and stability are highly important. Here, we describe protocols for tagging genes in Escherichia coli allowing for inducible degradation and transcriptional control of any soluble protein of interest....... The underlying molecular biology is based on the two cross-kingdom tools CRISPRi and the N-end rule for protein degradation. Genome editing is performed with the CRMAGE technology and randomization of the translational initiation region minimizes the polar effects of tag insertion. The approach has previously...... been applied for targeting proteins originating from essential operon-located genes and has potential to serve as a universal synthetic biology tool....

  2. Recent Insights into the Control of Human Papillomavirus (HPV) Genome Stability, Loss, and Degradation.

    Science.gov (United States)

    Fisher, Chris

    2015-01-01

    Most human papillomavirus (HPV) antiviral strategies have focused upon inhibiting viral DNA replication, but it is increasingly apparent that viral DNA levels can be chemically controlled by approaches that promote its instability. HPVs and other DNA viruses have a tenuous relationship with their hosts. They must replicate and hide from the DNA damage response (DDR) and innate immune systems, which serve to protect cells from foreign or "non-self" DNA, and yet they draft these same systems to support their life cycles. DNA binding antiviral agents promoting massive viral DNA instability and elimination are reviewed. Mechanistic studies of these agents have identified genetic antiviral enhancers and repressors, antiviral sensitizers, and host cell elements that protect and stabilize HPV genomes. Viral DNA degradation appears to be an important means of controlling HPV DNA levels in some cases, but the underlying mechanisms remain poorly understood. These findings may prove useful not only for understanding viral DNA persistence but also in devising future antiviral strategies.

  3. Recent Insights into the Control of Human Papillomavirus (HPV Genome Stability, Loss, and Degradation

    Directory of Open Access Journals (Sweden)

    Chris Fisher

    2015-01-01

    Full Text Available Most human papillomavirus (HPV antiviral strategies have focused upon inhibiting viral DNA replication, but it is increasingly apparent that viral DNA levels can be chemically controlled by approaches that promote its instability. HPVs and other DNA viruses have a tenuous relationship with their hosts. They must replicate and hide from the DNA damage response (DDR and innate immune systems, which serve to protect cells from foreign or "non-self" DNA, and yet they draft these same systems to support their life cycles. DNA binding antiviral agents promoting massive viral DNA instability and elimination are reviewed. Mechanistic studies of these agents have identified genetic antiviral enhancers and repressors, antiviral sensitizers, and host cell elements that protect and stabilize HPV genomes. Viral DNA degradation appears to be an important means of controlling HPV DNA levels in some cases, but the underlying mechanisms remain poorly understood. These findings may prove useful not only for understanding viral DNA persistence but also in devising future antiviral strategies.

  4. Drosophila MOF controls Checkpoint protein2 and regulates genomic stability during early embryogenesis.

    Science.gov (United States)

    Pushpavalli, Sreerangam N C V L; Sarkar, Arpita; Ramaiah, M Janaki; Chowdhury, Debabani Roy; Bhadra, Utpal; Pal-Bhadra, Manika

    2013-01-24

    In Drosophila embryos, checkpoints maintain genome stability by delaying cell cycle progression that allows time for damage repair or to complete DNA synthesis. Drosophila MOF, a member of MYST histone acetyl transferase is an essential component of male X hyperactivation process. Until recently its involvement in G2/M cell cycle arrest and defects in ionizing radiation induced DNA damage pathways was not well established. Drosophila MOF is highly expressed during early embryogenesis. In the present study we show that haplo-insufficiency of maternal MOF leads to spontaneous mitotic defects like mitotic asynchrony, mitotic catastrophe and chromatid bridges in the syncytial embryos. Such abnormal nuclei are eliminated and digested in the yolk tissues by nuclear fall out mechanism. MOF negatively regulates Drosophila checkpoint kinase 2 tumor suppressor homologue. In response to DNA damage the checkpoint gene Chk2 (Drosophila mnk) is activated in the mof mutants, there by causing centrosomal inactivation suggesting its role in response to genotoxic stress. A drastic decrease in the fall out nuclei in the syncytial embryos derived from mof¹/+; mnkp⁶/+ females further confirms the role of DNA damage response gene Chk2 to ensure the removal of abnormal nuclei from the embryonic precursor pool and maintain genome stability. The fact that mof mutants undergo DNA damage has been further elucidated by the increased number of single and double stranded DNA breaks. mof mutants exhibited genomic instability as evidenced by the occurance of frequent mitotic bridges in anaphase, asynchronous nuclear divisions, disruption of cytoskeleton, inactivation of centrosomes finally leading to DNA damage. Our findings are consistent to what has been reported earlier in mammals that; reduced levels of MOF resulted in increased genomic instability while total loss resulted in lethality. The study can be further extended using Drosophila as model system and carry out the interaction of MOF

  5. Drosophila MOF controls Checkpoint protein2 and regulates genomic stability during early embryogenesis

    Directory of Open Access Journals (Sweden)

    Pushpavalli Sreerangam NCVL

    2013-01-01

    Full Text Available Abstract Background In Drosophila embryos, checkpoints maintain genome stability by delaying cell cycle progression that allows time for damage repair or to complete DNA synthesis. Drosophila MOF, a member of MYST histone acetyl transferase is an essential component of male X hyperactivation process. Until recently its involvement in G2/M cell cycle arrest and defects in ionizing radiation induced DNA damage pathways was not well established. Results Drosophila MOF is highly expressed during early embryogenesis. In the present study we show that haplo-insufficiency of maternal MOF leads to spontaneous mitotic defects like mitotic asynchrony, mitotic catastrophe and chromatid bridges in the syncytial embryos. Such abnormal nuclei are eliminated and digested in the yolk tissues by nuclear fall out mechanism. MOF negatively regulates Drosophila checkpoint kinase 2 tumor suppressor homologue. In response to DNA damage the checkpoint gene Chk2 (Drosophila mnk is activated in the mof mutants, there by causing centrosomal inactivation suggesting its role in response to genotoxic stress. A drastic decrease in the fall out nuclei in the syncytial embryos derived from mof1/+; mnkp6/+ females further confirms the role of DNA damage response gene Chk2 to ensure the removal of abnormal nuclei from the embryonic precursor pool and maintain genome stability. The fact that mof mutants undergo DNA damage has been further elucidated by the increased number of single and double stranded DNA breaks. Conclusion mof mutants exhibited genomic instability as evidenced by the occurance of frequent mitotic bridges in anaphase, asynchronous nuclear divisions, disruption of cytoskeleton, inactivation of centrosomes finally leading to DNA damage. Our findings are consistent to what has been reported earlier in mammals that; reduced levels of MOF resulted in increased genomic instability while total loss resulted in lethality. The study can be further extended using

  6. The Aging Clock and Circadian Control of Metabolism and Genome Stability

    Directory of Open Access Journals (Sweden)

    Victoria P. Belancio

    2015-01-01

    Full Text Available It is widely accepted that aging is characterized by a gradual decline in the efficiency and accuracy of biological processes, leading to deterioration of physiological functions and development of age-associated diseases. Age-dependent accumulation of genomic instability and development of metabolic syndrome are well-recognized components of the aging phenotype, both of which have been extensively studied. Existing findings strongly support the view that the integrity of the cellular genome and metabolic function can be influenced by light at night (LAN and associated suppression of circadian melatonin production. While LAN is reported to accelerate aging by promoting age-associated carcinogenesis in several animal models, the specific molecular mechanism(s of its action are not fully understood. Here, we review literature supporting a connection between LAN-induced central circadian disruption of peripheral circadian rhythms and clock function, LINE-1 retrotransposon-associated genomic instability, metabolic deregulation, and aging. We propose that aging is a progressive decline in the stability, continuity and synchronization of multi-frequency oscillations in biological processes to a temporally disorganized state. By extension, healthy aging is the ability to maintain the most consistent, stable and entrainable rhythmicity and coordination of these oscillations, at the molecular, cellular, and systemic levels.

  7. Chromatin dynamics in genome stability

    DEFF Research Database (Denmark)

    Nair, Nidhi; Shoaib, Muhammad; Sørensen, Claus Storgaard

    2017-01-01

    Genomic DNA is compacted into chromatin through packaging with histone and non-histone proteins. Importantly, DNA accessibility is dynamically regulated to ensure genome stability. This is exemplified in the response to DNA damage where chromatin relaxation near genomic lesions serves to promote...... access of relevant enzymes to specific DNA regions for signaling and repair. Furthermore, recent data highlight genome maintenance roles of chromatin through the regulation of endogenous DNA-templated processes including transcription and replication. Here, we review research that shows the importance...... of chromatin structure regulation in maintaining genome integrity by multiple mechanisms including facilitating DNA repair and directly suppressing endogenous DNA damage....

  8. The role of zinc in genomic stability

    International Nuclear Information System (INIS)

    Sharif, Razinah; Thomas, Philip; Zalewski, Peter; Fenech, Michael

    2012-01-01

    Zinc (Zn) is an essential trace element required for maintaining both optimal human health and genomic stability. Zn plays a critical role in the regulation of DNA repair mechanisms, cell proliferation, differentiation and apoptosis involving the action of various transcriptional factors and DNA or RNA polymerases. Zn is an essential cofactor or structural component for important antioxidant defence proteins and DNA repair enzymes such as Cu/Zn SOD, OGG1, APE and PARP and may also affect activities of enzymes such as BHMT and MTR involved in methylation reactions in the folate-methionine cycle. This review focuses on the role of Zn in the maintenance of genome integrity and the effects of deficiency or excess on genomic stability events and cell death.

  9. Iron and genome stability: An update

    International Nuclear Information System (INIS)

    Prá, Daniel; Franke, Silvia Isabel Rech; Henriques, João Antonio Pêgas; Fenech, Michael

    2012-01-01

    Iron is an essential micronutrient which is required in a relatively narrow range for maintaining metabolic homeostasis and genome stability. Iron participates in oxygen transport and mitochondrial respiration as well as in antioxidant and nucleic acid metabolism. Iron deficiency impairs these biological pathways, leading to oxidative stress and possibly carcinogenesis. Iron overload has been linked to genome instability as well as to cancer risk increase, as seen in hereditary hemochromatosis. Iron is an extremely reactive transition metal that can interact with hydrogen peroxide to generate hydroxyl radicals that form the 8-hydroxy-guanine adduct, cause point mutations as well as DNA single and double strand breaks. Iron overload also induces DNA hypermethylation and can reduce telomere length. The current Recommended Dietary Allowances (RDA) for iron, according with Institute of Medicine Dietary Reference Intake (DRI), is based in the concept of preventing anemia, and ranges from 7 mg/day to 18 mg/day depending on life stage and gender. Pregnant women need 27 mg/day. The maximum safety level for iron intake, the Upper Level (UL), is 40–45 mg/day, based on the prevention of gastrointestinal distress associated to high iron intakes. Preliminary evidence indicates that 20 mg/day iron, an intake slightly higher than the RDA, may reduce the risk of gastrointestinal cancer in the elderly as well as increasing genome stability in lymphocytes of children and adolescents. Current dietary recommendations do not consider the concept of genome stability which is of concern because damage to the genome has been linked to the origin and progression of many diseases and is the most fundamental pathology. Given the importance of iron for homeostasis and its potential influence over genome stability and cancer it is recommended to conduct further studies that conclusively define these relationships.

  10. Iron and genome stability: An update

    Energy Technology Data Exchange (ETDEWEB)

    Pra, Daniel, E-mail: daniel_pra@yahoo.com [PPG em Promocao da Saude, Universidade de Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS (Brazil); PPG em Saude e Comportamento, Universidade Catolica de Pelotas, Pelotas, RS (Brazil); Franke, Silvia Isabel Rech [PPG em Promocao da Saude, Universidade de Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS (Brazil); Henriques, Joao Antonio Pegas [Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS (Brazil); Fenech, Michael [CSIRO Food and Nutritional Sciences, Adelaide, SA (Australia)

    2012-05-01

    Iron is an essential micronutrient which is required in a relatively narrow range for maintaining metabolic homeostasis and genome stability. Iron participates in oxygen transport and mitochondrial respiration as well as in antioxidant and nucleic acid metabolism. Iron deficiency impairs these biological pathways, leading to oxidative stress and possibly carcinogenesis. Iron overload has been linked to genome instability as well as to cancer risk increase, as seen in hereditary hemochromatosis. Iron is an extremely reactive transition metal that can interact with hydrogen peroxide to generate hydroxyl radicals that form the 8-hydroxy-guanine adduct, cause point mutations as well as DNA single and double strand breaks. Iron overload also induces DNA hypermethylation and can reduce telomere length. The current Recommended Dietary Allowances (RDA) for iron, according with Institute of Medicine Dietary Reference Intake (DRI), is based in the concept of preventing anemia, and ranges from 7 mg/day to 18 mg/day depending on life stage and gender. Pregnant women need 27 mg/day. The maximum safety level for iron intake, the Upper Level (UL), is 40-45 mg/day, based on the prevention of gastrointestinal distress associated to high iron intakes. Preliminary evidence indicates that 20 mg/day iron, an intake slightly higher than the RDA, may reduce the risk of gastrointestinal cancer in the elderly as well as increasing genome stability in lymphocytes of children and adolescents. Current dietary recommendations do not consider the concept of genome stability which is of concern because damage to the genome has been linked to the origin and progression of many diseases and is the most fundamental pathology. Given the importance of iron for homeostasis and its potential influence over genome stability and cancer it is recommended to conduct further studies that conclusively define these relationships.

  11. Clinically Applicable Inhibitors Impacting Genome Stability.

    Science.gov (United States)

    Prakash, Anu; Garcia-Moreno, Juan F; Brown, James A L; Bourke, Emer

    2018-05-13

    Advances in technology have facilitated the molecular profiling (genomic and transcriptomic) of tumours, and has led to improved stratification of patients and the individualisation of treatment regimes. To fully realize the potential of truly personalised treatment options, we need targeted therapies that precisely disrupt the compensatory pathways identified by profiling which allow tumours to survive or gain resistance to treatments. Here, we discuss recent advances in novel therapies that impact the genome (chromosomes and chromatin), pathways targeted and the stage of the pathways targeted. The current state of research will be discussed, with a focus on compounds that have advanced into trials (clinical and pre-clinical). We will discuss inhibitors of specific DNA damage responses and other genome stability pathways, including those in development, which are likely to synergistically combine with current therapeutic options. Tumour profiling data, combined with the knowledge of new treatments that affect the regulation of essential tumour signalling pathways, is revealing fundamental insights into cancer progression and resistance mechanisms. This is the forefront of the next evolution of advanced oncology medicine that will ultimately lead to improved survival and may, one day, result in many cancers becoming chronic conditions, rather than fatal diseases.

  12. Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization. | Office of Cancer Genomics

    Science.gov (United States)

    The CRISPR/Cas9 system is a powerful tool for studying gene function. Here, we describe a method that allows temporal control of CRISPR/Cas9 activity based on conditional Cas9 destabilization. We demonstrate that fusing an FKBP12-derived destabilizing domain to Cas9 (DD-Cas9) enables conditional Cas9 expression and temporal control of gene editing in the presence of an FKBP12 synthetic ligand. This system can be easily adapted to co-express, from the same promoter, DD-Cas9 with any other gene of interest without co-modulation of the latter.

  13. RTEL1 maintains genomic stability by suppressing homologous recombination.

    Science.gov (United States)

    Barber, Louise J; Youds, Jillian L; Ward, Jordan D; McIlwraith, Michael J; O'Neil, Nigel J; Petalcorin, Mark I R; Martin, Julie S; Collis, Spencer J; Cantor, Sharon B; Auclair, Melissa; Tissenbaum, Heidi; West, Stephen C; Rose, Ann M; Boulton, Simon J

    2008-10-17

    Homologous recombination (HR) is an important conserved process for DNA repair and ensures maintenance of genome integrity. Inappropriate HR causes gross chromosomal rearrangements and tumorigenesis in mammals. In yeast, the Srs2 helicase eliminates inappropriate recombination events, but the functional equivalent of Srs2 in higher eukaryotes has been elusive. Here, we identify C. elegans RTEL-1 as a functional analog of Srs2 and describe its vertebrate counterpart, RTEL1, which is required for genome stability and tumor avoidance. We find that rtel-1 mutant worms and RTEL1-depleted human cells share characteristic phenotypes with yeast srs2 mutants: lethality upon deletion of the sgs1/BLM homolog, hyperrecombination, and DNA damage sensitivity. In vitro, purified human RTEL1 antagonizes HR by promoting the disassembly of D loop recombination intermediates in a reaction dependent upon ATP hydrolysis. We propose that loss of HR control after deregulation of RTEL1 may be a critical event that drives genome instability and cancer.

  14. Genomic stability of adipogenic human adenovirus 36.

    Science.gov (United States)

    Nam, J-H; Na, H-N; Atkinson, R L; Dhurandhar, N V

    2014-02-01

    Human adenovirus Ad36 increases adiposity in several animal models, including rodents and non-human primates. Importantly, Ad36 is associated with human obesity, which has prompted research to understand its epidemiology and to develop a vaccine to prevent a subgroup of obesity. For this purpose, understanding the genomic stability of Ad36 in vivo and in vitro infections is critical. Here, we examined whether in vitro cell passaging over a 14-year period introduced any genetic variation in Ad36. We sequenced the whole genome of Ad36-which was plaque purified in 1998 from the original strain obtained from American Type Culture Collection, and passaged approximately 12 times over the past 14 years (Ad36-2012). This DNA sequence was compared with a previously published sequence of Ad36 likely obtained from the same source (Ad36-1988). Compared with Ad36-1988, only two nucleotides were altered in Ad36-2012: a T insertion at nucleotide 1862, which may induce early termination of the E1B viral protein, and a T➝C transition at nucleotide 26 136. Virus with the T insertion (designated Ad36-2012-T6) was mixed with wild-type virus lacking the T insertion (designated Ad36-2012-T5) in the viral stock. The transition at nucleotide 26 136 does not change the encoded amino acid (aspartic acid) in the pVIII viral protein. The rate of genetic variation in Ad36 is ∼2.37 × 10(-6) mutations/nucleotide/passage. Of particular importance, there were no mutations in the E4orf1 gene, the critical gene for producing obesity. This very-low-variation rate should reduce concerns about genetic variability when developing Ad36 vaccines or developing assays for detecting Ad36 infection in populations.

  15. Genome Stability Maintenance in Naked Mole-Rat.

    Science.gov (United States)

    Petruseva, I O; Evdokimov, A N; Lavrik, O I

    2017-01-01

    The naked mole-rat ( Heterocephalus glaber ) is one of the most promising models used to study genome maintenance systems, including the effective repair of damage to DNA. The naked mole-rat is the longest lived rodent species, which is extraordinarily resistant to cancer and has a number of other unique phenotypic traits. For at least 80% of its lifespan, this animal shows no signs of aging or any increased likelihood of death and retains the ability to reproduce. The naked mole-rat draws the heightened attention of researchers who study the molecular basis of lengthy lifespan and cancer resistance. Despite the fact that the naked mole-rat lives under genotoxic stress conditions (oxidative, etc.), the main characteristics of its genome and proteome are a high stability and effective functioning. Replicative senescence in the somatic cells of naked mole-rats is missing, while an additional p53/pRb-dependent mechanism of early contact inhibition has been revealed in its fibroblasts, which controls cell proliferation and its mechanism of arf- dependent aging. The unique traits of phenotypic and molecular adaptations found in the naked mole-rat speak to a high stability and effective functioning of the molecular machinery that counteract damage accumulation in its genome. This review analyzes existing results in the study of the molecular basis of longevity and high cancer resistance in naked mole-rats.

  16. Organelles genome stability of wheat plantlets produced by anther ...

    African Journals Online (AJOL)

    Yomi

    2012-03-15

    Mar 15, 2012 ... 1Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa' Applied University, ... genetic stability of wheat organelles genomes for plantlets produced by anther culture using restriction ..... of transgenic plants.

  17. Genomic stability during cellular reprogramming: Mission impossible?

    Energy Technology Data Exchange (ETDEWEB)

    Joest, Mathieu von; Búa Aguín, Sabela; Li, Han, E-mail: han.li@pasteur.fr

    2016-06-15

    The generation of induced pluripotent stem cells (iPSCs) from adult somatic cells is one of the most exciting discoveries in recent biomedical research. It holds tremendous potential in drug discovery and regenerative medicine. However, a series of reports highlighting genomic instability in iPSCs raises concerns about their clinical application. Although the mechanisms cause genomic instability during cellular reprogramming are largely unknown, several potential sources have been suggested. This review summarizes current knowledge on this active research field and discusses the latest efforts to alleviate the genomic insults during cellular reprogramming to generate iPSCs with enhanced quality and safety.

  18. ATM signaling and genomic stability in response to DNA damage

    International Nuclear Information System (INIS)

    Lavin, Martin F.; Birrell, Geoff; Chen, Philip; Kozlov, Sergei; Scott, Shaun; Gueven, Nuri

    2005-01-01

    DNA double strand breaks represent the most threatening lesion to the integrity of the genome in cells exposed to ionizing radiation and radiomimetic chemicals. Those breaks are recognized, signaled to cell cycle checkpoints and repaired by protein complexes. The product of the gene (ATM) mutated in the human genetic disorder ataxia-telangiectasia (A-T) plays a central role in the recognition and signaling of DNA damage. ATM is one of an ever growing number of proteins which when mutated compromise the stability of the genome and predispose to tumour development. Mechanisms for recognising double strand breaks in DNA, maintaining genome stability and minimizing risk of cancer are discussed

  19. DNA Precursor Metabolism and Mitochondrial Genome Stability

    National Research Council Canada - National Science Library

    Mathews, Christopher K

    2003-01-01

    ...) metabolism and mutagenesis in the mitochondrial genome. Specific contributions include: (1) We found that conditions altering the normal balance among the four dNTP pools within the mitochondrion stimulate both point and deletion mutagenesis...

  20. Genome Stability Pathways in Head and Neck Cancers

    Directory of Open Access Journals (Sweden)

    Glenn Jenkins

    2013-01-01

    Full Text Available Genomic instability underlies the transformation of host cells toward malignancy, promotes development of invasion and metastasis and shapes the response of established cancer to treatment. In this review, we discuss recent advances in our understanding of genomic stability in squamous cell carcinoma of the head and neck (HNSCC, with an emphasis on DNA repair pathways. HNSCC is characterized by distinct profiles in genome stability between similarly staged cancers that are reflected in risk, treatment response and outcomes. Defective DNA repair generates chromosomal derangement that can cause subsequent alterations in gene expression, and is a hallmark of progression toward carcinoma. Variable functionality of an increasing spectrum of repair gene polymorphisms is associated with increased cancer risk, while aetiological factors such as human papillomavirus, tobacco and alcohol induce significantly different behaviour in induced malignancy, underpinned by differences in genomic stability. Targeted inhibition of signalling receptors has proven to be a clinically-validated therapy, and protein expression of other DNA repair and signalling molecules associated with cancer behaviour could potentially provide a more refined clinical model for prognosis and treatment prediction. Development and expansion of current genomic stability models is furthering our understanding of HNSCC pathophysiology and uncovering new, promising treatment strategies.

  1. Genome Stability Pathways in Head and Neck Cancers

    Science.gov (United States)

    O'Byrne, Kenneth J.; Panizza, Benedict; Richard, Derek J.

    2013-01-01

    Genomic instability underlies the transformation of host cells toward malignancy, promotes development of invasion and metastasis and shapes the response of established cancer to treatment. In this review, we discuss recent advances in our understanding of genomic stability in squamous cell carcinoma of the head and neck (HNSCC), with an emphasis on DNA repair pathways. HNSCC is characterized by distinct profiles in genome stability between similarly staged cancers that are reflected in risk, treatment response and outcomes. Defective DNA repair generates chromosomal derangement that can cause subsequent alterations in gene expression, and is a hallmark of progression toward carcinoma. Variable functionality of an increasing spectrum of repair gene polymorphisms is associated with increased cancer risk, while aetiological factors such as human papillomavirus, tobacco and alcohol induce significantly different behaviour in induced malignancy, underpinned by differences in genomic stability. Targeted inhibition of signalling receptors has proven to be a clinically-validated therapy, and protein expression of other DNA repair and signalling molecules associated with cancer behaviour could potentially provide a more refined clinical model for prognosis and treatment prediction. Development and expansion of current genomic stability models is furthering our understanding of HNSCC pathophysiology and uncovering new, promising treatment strategies. PMID:24364026

  2. Aspects of ribonucleotide reductase regulation and genome stability

    DEFF Research Database (Denmark)

    Nielsen, Helena Berner Nedergaard

    yeast, and Sml1, Hug1, and Dif1 in budding yeast. An elevated, as well as a reduced dNTP pool is shown to lead to an increase in spontaneous mutation rates, hence regulation of RNR is very important in order to maintain genomic stability. No human inhibitory proteins have yet been identified to regulate....... RNR consists of two subunits: R1 and R2, both of which are essential. The activity of RNR is strictly regulated to control the dNTP pool, both by allosteric feedback control and transcriptional and translational controls. Four inhibitory proteins of RNR have been identified in yeast: Spd1 in fission...... the human RNR enzyme. In this study regulation of human RNR was investigated using a fission yeast strain that depended solely on the human genes of R1 and R2 for dNTP synthesis. Even though this strain could grow like wild-type fission yeast it was hypersensitive to hydroxyurea (HU) and depended...

  3. Seeds as emerging hotspot for maintenance of genome stability

    Czech Academy of Sciences Publication Activity Database

    Diaz, Mariana; Pečinka, Aleš

    2017-01-01

    Roč. 82, č. 5 (2017), s. 467-470 ISSN 0011-4545 Institutional support: RVO:61389030 Keywords : Chromatin * Chromosome * DNA damage repair * Genome stability * Seed * Structural maintenance of chromosome Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Genetics and heredity (medical genetics to be 3) Impact factor: 0.913, year: 2016

  4. Short and long-term genome stability analysis of prokaryotic genomes.

    Science.gov (United States)

    Brilli, Matteo; Liò, Pietro; Lacroix, Vincent; Sagot, Marie-France

    2013-05-08

    Gene organization dynamics is actively studied because it provides useful evolutionary information, makes functional annotation easier and often enables to characterize pathogens. There is therefore a strong interest in understanding the variability of this trait and the possible correlations with life-style. Two kinds of events affect genome organization: on one hand translocations and recombinations change the relative position of genes shared by two genomes (i.e. the backbone gene order); on the other, insertions and deletions leave the backbone gene order unchanged but they alter the gene neighborhoods by breaking the syntenic regions. A complete picture about genome organization evolution therefore requires to account for both kinds of events. We developed an approach where we model chromosomes as graphs on which we compute different stability estimators; we consider genome rearrangements as well as the effect of gene insertions and deletions. In a first part of the paper, we fit a measure of backbone gene order conservation (hereinafter called backbone stability) against phylogenetic distance for over 3000 genome comparisons, improving existing models for the divergence in time of backbone stability. Intra- and inter-specific comparisons were treated separately to focus on different time-scales. The use of multiple genomes of a same species allowed to identify genomes with diverging gene order with respect to their conspecific. The inter-species analysis indicates that pathogens are more often unstable with respect to non-pathogens. In a second part of the text, we show that in pathogens, gene content dynamics (insertions and deletions) have a much more dramatic effect on genome organization stability than backbone rearrangements. In this work, we studied genome organization divergence taking into account the contribution of both genome order rearrangements and genome content dynamics. By studying species with multiple sequenced genomes available, we were

  5. Control of locomotor stability in stabilizing and destabilizing environments.

    Science.gov (United States)

    Wu, Mengnan/Mary; Brown, Geoffrey; Gordon, Keith E

    2017-06-01

    To develop effective interventions targeting locomotor stability, it is crucial to understand how people control and modify gait in response to changes in stabilization requirements. Our purpose was to examine how individuals with and without incomplete spinal cord injury (iSCI) control lateral stability in haptic walking environments that increase or decrease stabilization demands. We hypothesized that people would adapt to walking in a predictable, stabilizing viscous force field and unpredictable destabilizing force field by increasing and decreasing feedforward control of lateral stability, respectively. Adaptations in feedforward control were measured using after-effects when fields were removed. Both groups significantly (pfeedforward adaptions to increase control of lateral stability. In contrast, in the destabilizing field, non-impaired subjects increased movement variability (p0.05). When the destabilizing field was removed, increases in movement variability persisted (pfeedforward decreases in resistance to perturbations. Published by Elsevier B.V.

  6. Genome-Wide Analysis of Grain Yield Stability and Environmental Interactions in a Multiparental Soybean Population

    Directory of Open Access Journals (Sweden)

    Alencar Xavier

    2018-02-01

    Full Text Available Genetic improvement toward optimized and stable agronomic performance of soybean genotypes is desirable for food security. Understanding how genotypes perform in different environmental conditions helps breeders develop sustainable cultivars adapted to target regions. Complex traits of importance are known to be controlled by a large number of genomic regions with small effects whose magnitude and direction are modulated by environmental factors. Knowledge of the constraints and undesirable effects resulting from genotype by environmental interactions is a key objective in improving selection procedures in soybean breeding programs. In this study, the genetic basis of soybean grain yield responsiveness to environmental factors was examined in a large soybean nested association population. For this, a genome-wide association to performance stability estimates generated from a Finlay-Wilkinson analysis and the inclusion of the interaction between marker genotypes and environmental factors was implemented. Genomic footprints were investigated by analysis and meta-analysis using a recently published multiparent model. Results indicated that specific soybean genomic regions were associated with stability, and that multiplicative interactions were present between environments and genetic background. Seven genomic regions in six chromosomes were identified as being associated with genotype-by-environment interactions. This study provides insight into genomic assisted breeding aimed at achieving a more stable agronomic performance of soybean, and documented opportunities to exploit genomic regions that were specifically associated with interactions involving environments and subpopulations.

  7. Bloom syndrome ortholog HIM-6 maintains genomic stability in C. elegans.

    Science.gov (United States)

    Grabowski, Melissa M; Svrzikapa, Nenad; Tissenbaum, Heidi A

    2005-12-01

    Bloom syndrome is caused by mutation of the Bloom helicase (BLM), a member of the RecQ helicase family. Loss of BLM function results in genomic instability that causes a high incidence of cancer. It has been demonstrated that BLM is important for maintaining genomic stability by playing a role in DNA recombination and repair; however, the exact function of BLM is not clearly understood. To determine the mechanism by which BLM controls genomic stability in vivo, we examined the phenotypes caused by mutation of the C. elegans BLM helicase ortholog, HIM-6. We find that the loss of HIM-6 leads to genomic instability as evidenced by an increased number of genomic insertions and deletions, which results in visible random mutant phenotypes. In addition to the mutator phenotype, him-6 mutants have a low brood size, a high incidence of males, a shortened life span, and an increased amount of germ line apoptosis. Upon exposure to high temperature, him-6 mutants that are serially passed become sterile demonstrating a mortal germ line phenotype. Our data suggest a model in which loss of HIM-6 results in genomic instability due to an increased number of DNA lesions, which either cannot be repaired and/or are introduced by low fidelity recombination events. The increased level of genomic instability that leads to him-6(ok412) mutants having a shortened life span.

  8. Epigenetic regulation of ageing: linking environmental inputs to genomic stability

    Science.gov (United States)

    Benayoun, Bérénice A.; Pollina, Elizabeth A.; Brunet, Anne

    2016-01-01

    Preface Ageing is affected by both genetic and non-genetic factors. Here, we review the chromatin-based epigenetic changes that occur during ageing, the role of chromatin modifiers in modulating lifespan and the importance of epigenetic signatures as biomarkers of ageing. We also discuss how epigenome remodeling by environmental stimuli impacts several aspects of transcription and genomic stability, with important consequences on longevity, and outline epigenetic differences between the ‘mortal soma’ and the ‘immortal germline’. Finally, we discuss the inheritance of ageing characteristics and potential chromatin-based strategies to delay or reverse hallmarks of ageing or age-related diseases. PMID:26373265

  9. Chromosome End Repair and Genome Stability in Plasmodium falciparum.

    Science.gov (United States)

    Calhoun, Susannah F; Reed, Jake; Alexander, Noah; Mason, Christopher E; Deitsch, Kirk W; Kirkman, Laura A

    2017-08-08

    The human malaria parasite Plasmodium falciparum replicates within circulating red blood cells, where it is subjected to conditions that frequently cause DNA damage. The repair of DNA double-stranded breaks (DSBs) is thought to rely almost exclusively on homologous recombination (HR), due to a lack of efficient nonhomologous end joining. However, given that the parasite is haploid during this stage of its life cycle, the mechanisms involved in maintaining genome stability are poorly understood. Of particular interest are the subtelomeric regions of the chromosomes, which contain the majority of the multicopy variant antigen-encoding genes responsible for virulence and disease severity. Here, we show that parasites utilize a competitive balance between de novo telomere addition, also called "telomere healing," and HR to stabilize chromosome ends. Products of both repair pathways were observed in response to DSBs that occurred spontaneously during routine in vitro culture or resulted from experimentally induced DSBs, demonstrating that both pathways are active in repairing DSBs within subtelomeric regions and that the pathway utilized was determined by the DNA sequences immediately surrounding the break. In combination, these two repair pathways enable parasites to efficiently maintain chromosome stability while also contributing to the generation of genetic diversity. IMPORTANCE Malaria is a major global health threat, causing approximately 430,000 deaths annually. This mosquito-transmitted disease is caused by Plasmodium parasites, with infection with the species Plasmodium falciparum being the most lethal. Mechanisms underlying DNA repair and maintenance of genome integrity in P. falciparum are not well understood and represent a gap in our understanding of how parasites survive the hostile environment of their vertebrate and insect hosts. Our work examines DNA repair in real time by using single-molecule real-time (SMRT) sequencing focused on the subtelomeric

  10. New stability and stabilization for switched neutral control systems

    International Nuclear Information System (INIS)

    Xiong Lianglin; Zhong Shouming; Ye Mao; Wu Shiliang

    2009-01-01

    This paper concerns stability and stabilization issues for switched neutral systems and presents new classes of piecewise Lyapunov functionals and multiple Lyapunov functionals, based on which, two new switching rules are introduced to stabilize the neutral systems. One switching rule is designed from the solution of the so-called Lyapunov-Metzler linear matrix inequalities. The other is based on the determination of average dwell time computed from a new class of linear matrix inequalities (LMIs). And then, state-feedback control is derived for the switched neutral control system mainly based on the state switching rules. Finally, three examples are given to demonstrate the effectiveness of the proposed method.

  11. Chromosome End Repair and Genome Stability in Plasmodium falciparum

    Directory of Open Access Journals (Sweden)

    Susannah F. Calhoun

    2017-08-01

    Full Text Available The human malaria parasite Plasmodium falciparum replicates within circulating red blood cells, where it is subjected to conditions that frequently cause DNA damage. The repair of DNA double-stranded breaks (DSBs is thought to rely almost exclusively on homologous recombination (HR, due to a lack of efficient nonhomologous end joining. However, given that the parasite is haploid during this stage of its life cycle, the mechanisms involved in maintaining genome stability are poorly understood. Of particular interest are the subtelomeric regions of the chromosomes, which contain the majority of the multicopy variant antigen-encoding genes responsible for virulence and disease severity. Here, we show that parasites utilize a competitive balance between de novo telomere addition, also called “telomere healing,” and HR to stabilize chromosome ends. Products of both repair pathways were observed in response to DSBs that occurred spontaneously during routine in vitro culture or resulted from experimentally induced DSBs, demonstrating that both pathways are active in repairing DSBs within subtelomeric regions and that the pathway utilized was determined by the DNA sequences immediately surrounding the break. In combination, these two repair pathways enable parasites to efficiently maintain chromosome stability while also contributing to the generation of genetic diversity.

  12. Initial processes of radiation effects on genomic stability

    International Nuclear Information System (INIS)

    Herve du Penhoat, Marie-Anne; Touati, Alain; Sage, Evelyne; Houee-Levin, Chantal; Lacombe, Sandrine; Politis, Marie-Françoise; Gaigeot, Marie-Pierre; Spezia, Riccardo; Vuilleumier, Rodolphe; Wien, Frank; Fujii, Kentaro; Yokoya, Akinari; Shikazono, Naoya

    2014-01-01

    We describe the progresses and current status of the REIMEI study on 'Initial processes of radiation effects on genomic stability', which is a new collaborative project between France and Japan. The theoretical study on the fragmentation pattern of doubly ionized deoxyribose has reached a new stage focusing on the effect of water molecules surrounding the deoxyribose molecule. In order to substantiate the theoretical predictions, new devices have been installed in a SPring-8 beamline. To explore the origin of signal transduction of DNA repair processes, a preliminary CD measurement in the far UV region was performed to study histone conformation changes. Based on these results, we will propose a new experimental subject in SOLEIL synchrotron to investigate the conformational changes of phosphorylated histone. Preliminary experiments using mammalian cell free extract have also been performed focusing on the role of DNA polymerases on the mutagenic potential of clustered DNA lesions. (author)

  13. Cooperativity of the SUMO and Ubiquitin Pathways in Genome Stability

    Directory of Open Access Journals (Sweden)

    Minghua Nie

    2016-02-01

    Full Text Available Covalent attachment of ubiquitin (Ub or SUMO to DNA repair proteins plays critical roles in maintaining genome stability. These structurally related polypeptides can be viewed as distinct road signs, with each being read by specific protein interaction motifs. Therefore, via their interactions with selective readers in the proteome, ubiquitin and SUMO can elicit distinct cellular responses, such as directing DNA lesions into different repair pathways. On the other hand, through the action of the SUMO-targeted ubiquitin ligase (STUbL family proteins, ubiquitin and SUMO can cooperate in the form of a hybrid signal. These mixed SUMO-ubiquitin chains recruit “effector” proteins such as the AAA+ ATPase Cdc48/p97-Ufd1-Npl4 complex that contain both ubiquitin and SUMO interaction motifs. This review will summarize recent key findings on collaborative and distinct roles that ubiquitin and SUMO play in orchestrating DNA damage responses.

  14. MATLAB Stability and Control Toolbox Trim and Static Stability Module

    Science.gov (United States)

    Kenny, Sean P.; Crespo, Luis

    2012-01-01

    MATLAB Stability and Control Toolbox (MASCOT) utilizes geometric, aerodynamic, and inertial inputs to calculate air vehicle stability in a variety of critical flight conditions. The code is based on fundamental, non-linear equations of motion and is able to translate results into a qualitative, graphical scale useful to the non-expert. MASCOT was created to provide the conceptual aircraft designer accurate predictions of air vehicle stability and control characteristics. The code takes as input mass property data in the form of an inertia tensor, aerodynamic loading data, and propulsion (i.e. thrust) loading data. Using fundamental nonlinear equations of motion, MASCOT then calculates vehicle trim and static stability data for the desired flight condition(s). Available flight conditions include six horizontal and six landing rotation conditions with varying options for engine out, crosswind, and sideslip, plus three take-off rotation conditions. Results are displayed through a unique graphical interface developed to provide the non-stability and control expert conceptual design engineer a qualitative scale indicating whether the vehicle has acceptable, marginal, or unacceptable static stability characteristics. If desired, the user can also examine the detailed, quantitative results.

  15. Matlab Stability and Control Toolbox: Trim and Static Stability Module

    Science.gov (United States)

    Crespo, Luis G.; Kenny, Sean P.

    2006-01-01

    This paper presents the technical background of the Trim and Static module of the Matlab Stability and Control Toolbox. This module performs a low-fidelity stability and control assessment of an aircraft model for a set of flight critical conditions. This is attained by determining if the control authority available for trim is sufficient and if the static stability characteristics are adequate. These conditions can be selected from a prescribed set or can be specified to meet particular requirements. The prescribed set of conditions includes horizontal flight, take-off rotation, landing flare, steady roll, steady turn and pull-up/ push-over flight, for which several operating conditions can be specified. A mathematical model was developed allowing for six-dimensional trim, adjustable inertial properties, asymmetric vehicle layouts, arbitrary number of engines, multi-axial thrust vectoring, engine(s)-out conditions, crosswind and gyroscopic effects.

  16. Editorial: 3Rs tightly intertwined to maintain genome stability

    DEFF Research Database (Denmark)

    Lisby, Michael; Mortensen, Uffe H.

    2017-01-01

    , replication of damaged DNA results in stalled replication forks that await DNA damage repair before replication can be resumed. In turn, the repair of most lesions depends on processes involving DNA synthesis. At the same time, the stalled forks may engage in recombination, either as part of a controlled...... repair process or by accident, just because it can, with the risk of producing genome rearrangements and loss of heterozygosity. The set of reviews presented in this thematic issue (https://academic-oup-com.proxy.findit.dtu.dk/femsyr/pages/replication_recombination_and_repair) of FEMSYR has been selected...

  17. Hybrid Intelligent Control for Submarine Stabilization

    Directory of Open Access Journals (Sweden)

    Minghui Wang

    2013-05-01

    Full Text Available Abstract While sailing near the sea surface, submarines will often undergo rolling motion caused by wave disturbance. Fierce rolling motion seriously affects their normal operation and even threatens their security. We propose a new control method for roll stabilization. This paper studies hybrid intelligent control combining a fuzzy control, a neural network and extension control technology. Every control strategy can achieve the ideal control effect within the scope of its effective control. The neuro-fuzzy control strategy is used to improve the robustness of the controller. The speed control strategy and the course control strategy are conducted to extend the control range. The paper also proposes the design of the controller and carries out the simulation experiment in different sea conditions. The simulation results show that the control method proposed can indeed effectively improve the control performance of submarine stabilization.

  18. Power system stability modelling, analysis and control

    CERN Document Server

    Sallam, Abdelhay A

    2015-01-01

    This book provides a comprehensive treatment of the subject from both a physical and mathematical perspective and covers a range of topics including modelling, computation of load flow in the transmission grid, stability analysis under both steady-state and disturbed conditions, and appropriate controls to enhance stability.

  19. Epigenetic control of mobile DNA as an interface between experience and genome change

    Directory of Open Access Journals (Sweden)

    James A. Shapiro

    2014-04-01

    Full Text Available Mobile DNA in the genome is subject to RNA-targeted epigenetic control. This control regulates the activity of transposons, retrotransposons and genomic proviruses. Many different life history experiences alter the activities of mobile DNA and the expression of genetic loci regulated by nearby insertions. The same experiences induce alterations in epigenetic formatting and lead to trans-generational modifications of genome expression and stability. These observations lead to the hypothesis that epigenetic formatting directed by non-coding RNA provides a molecular interface between life history events and genome alteration.

  20. Plants from Chernobyl zone could shed light on genome stability in radioactive environment

    Science.gov (United States)

    Shevchenko, Galina; Talalaiev, Oleksandr; Doonan, John

    2016-07-01

    For nearly 30 years, despite of chronic radiation, flora in Chernobyl zone continue to flourish, evidencing the adaptation of plants to such an environment. Keeping in mind interplanetary missions, this phenomenon is a challenge for plant space research since it highlights the possible mechanisms of genome protection and stabilization in harmful environment. Plants are sessile organisms and, contrary to animals, could not escape the external impact. Therefore, plants should evolve the robust system allowing DNA-protection against damage, which is of special interest. Our investigations show that Arabidopsis thaliana from Chernobyl zone tolerate radiomimetics and heavy metals better than control plants from non-polluted areas. Besides, its genome is less affected by such mutagens. qPCR investigations have revealed up-regulation of some genes involved in DNA damage response. In particular, expression of ATR is increased slightly and downstream expression of CycB1:1 gene is increased significantly after bleomycin treatment suggesting role of ATR-dependent pathway in genome stabilization. Several DNA repair pathways are known to exist in plants. We continue investigations on gene expression from different DNA repair pathways as well as cell cycle regulation and investigation of PCD hallmarks in order to reveal the mechanism of plant tolerance to radiation environment. Our investigations provide unique information for space researchers working on biotechnology of radiation tolerant plants.

  1. Genetic stability of pestivirus genomes cloned into BACs

    DEFF Research Database (Denmark)

    Rasmussen, Thomas Bruun; Reimann, Ilona; Uttenthal, Åse

    pestivirus genomes to demonstrate the suitability of the BAC vector for harbouring pestivirus genomes. Two BAC clones, comprising the complete genomes of BDV Gifhorn (pBeloGif3) and CSFV Paderborn (pBeloPader10) were passaged 15 times in E.coli representing at least 360 bacteria generations. From 15th...

  2. Stability Constraints for Robust Model Predictive Control

    Directory of Open Access Journals (Sweden)

    Amanda G. S. Ottoni

    2015-01-01

    Full Text Available This paper proposes an approach for the robust stabilization of systems controlled by MPC strategies. Uncertain SISO linear systems with box-bounded parametric uncertainties are considered. The proposed approach delivers some constraints on the control inputs which impose sufficient conditions for the convergence of the system output. These stability constraints can be included in the set of constraints dealt with by existing MPC design strategies, in this way leading to the “robustification” of the MPC.

  3. Can electronic stability control replace studded tyres?

    DEFF Research Database (Denmark)

    Elvik, Rune

    2015-01-01

    Highlights • Electronic stability control can substitute studded tyres. • This makes it easier to discourage the use of studded tyres. • A certain level of use of studded tyres makes roads less slippery.......Highlights • Electronic stability control can substitute studded tyres. • This makes it easier to discourage the use of studded tyres. • A certain level of use of studded tyres makes roads less slippery....

  4. Controllability and stabilization of parabolic equations

    CERN Document Server

    Barbu, Viorel

    2018-01-01

    This monograph presents controllability and stabilization methods in control theory that solve parabolic boundary value problems. Starting from foundational questions on Carleman inequalities for linear parabolic equations, the author addresses the controllability of parabolic equations on a variety of domains and the spectral decomposition technique for representing them. This method is, in fact, designed for use in a wider class of parabolic systems that include the heat and diffusion equations. Later chapters develop another process that employs stabilizing feedback controllers with a finite number of unstable modes, with special attention given to its use in the boundary stabilization of Navier–Stokes equations for the motion of viscous fluid. In turn, these applied methods are used to explore related topics like the exact controllability of stochastic parabolic equations with linear multiplicative noise. Intended for graduate students and researchers working on control problems involving nonlinear diff...

  5. OPTIMAL CONTROL FOR ELECTRIC VEHICLE STABILIZATION

    Directory of Open Access Journals (Sweden)

    MARIAN GAICEANU

    2016-01-01

    Full Text Available This main objective of the paper is to stabilize an electric vehicle in optimal manner to a step lane change maneuver. To define the mathematical model of the vehicle, the rigid body moving on a plane is taken into account. An optimal lane keeping controller delivers the adequate angles in order to stabilize the vehicle’s trajectory in an optimal way. Two degree of freedom linear bicycle model is adopted as vehicle model, consisting of lateral and yaw motion equations. The proposed control maintains the lateral stability by taking the feedback information from the vehicle transducers. In this way only the lateral vehicle’s dynamics are enough to considerate. Based on the obtained linear mathematical model the quadratic optimal control is designed in order to maintain the lateral stability of the electric vehicle. The numerical simulation results demonstrate the feasibility of the proposed solution.

  6. Photobiomodulation effects on mRNA levels from genomic and chromosome stabilization genes in injured muscle.

    Science.gov (United States)

    da Silva Neto Trajano, Larissa Alexsandra; Trajano, Eduardo Tavares Lima; da Silva Sergio, Luiz Philippe; Teixeira, Adilson Fonseca; Mencalha, Andre Luiz; Stumbo, Ana Carolina; de Souza da Fonseca, Adenilson

    2018-04-26

    Muscle injuries are the most prevalent type of injury in sports. A great number of athletes have relapsed in muscle injuries not being treated properly. Photobiomodulation therapy is an inexpensive and safe technique with many benefits in muscle injury treatment. However, little has been explored about the infrared laser effects on DNA and telomeres in muscle injuries. Thus, the aim of this study was to evaluate photobiomodulation effects on mRNA relative levels from genes related to telomere and genomic stabilization in injured muscle. Wistar male rats were randomly divided into six groups: control, laser 25 mW, laser 75 mW, injury, injury laser 25 mW, and injury laser 75 mW. Photobiomodulation was performed with 904 nm, 3 J/cm 2 at 25 or 75 mW. Cryoinjury was induced by two applications of a metal probe cooled in liquid nitrogen directly on the tibialis anterior muscle. After euthanasia, skeletal muscle samples were withdrawn and total RNA extracted for evaluation of mRNA levels from genomic (ATM and p53) and chromosome stabilization (TRF1 and TRF2) genes by real-time quantitative polymerization chain reaction. Data show that photobiomodulation reduces the mRNA levels from ATM and p53, as well reduces mRNA levels from TRF1 and TRF2 at 25 and 75 mW in injured skeletal muscle. In conclusion, photobiomodulation alters mRNA relative levels from genes related to genomic and telomere stabilization in injured skeletal muscle.

  7. Virtual sensors for advanced vehicle stability control

    NARCIS (Netherlands)

    Leenen, R.; Schouten, H.

    2010-01-01

    Advanced vehicle control technologies provide a great potential to further improve vehicle handling, ride and safety. The goal of this research is to demonstrate the added value of the TNO Vehicle State Estimation module to integrated active safety. State-of-the-art Electronic Stability Control

  8. Stability of digital feedback control systems

    Directory of Open Access Journals (Sweden)

    Larkin Eugene

    2018-01-01

    Lag time characteristics are used for investigation of stability of linear systems. Digital PID controller is divided onto linear part, which is realized with a soft and pure lag unit, which is realized with both hardware and software. With use notions amplitude and phase margins, condition for stability of system functioning are obtained. Theoretical results are confirm with computer experiment carried out on the third-order system.

  9. Maintaining Genome Stability: The Role of Helicases and Deaminases

    Science.gov (United States)

    2008-07-01

    Errors in duplicating DNA can result in genomic instability, leading to various human diseases, such as cancer, immune system disorder, muscle dystrophy ...as cancer, immune system disorder, muscle dystrophy , and neurodegenerations. Thus, maintaining genomic integrity is vital to the normal growth of...31–38. Eberharter, A., R. Ferreira and P. Becker , 2005 Dynamic chro- matin: concerted nucleosome remodelling and acetylation. Biol. Chem. 386: 745

  10. MASCOT - MATLAB Stability and Control Toolbox

    Science.gov (United States)

    Kenny, Sean; Crespo, Luis

    2011-01-01

    MASCOT software was created to provide the conceptual aircraft designer accurate predictions of air vehicle stability and control characteristics. The code takes as input mass property data in the form of an inertia tensor, aerodynamic loading data, and propulsion (i.e. thrust) loading data. Using fundamental non-linear equations of motion, MASCOT then calculates vehicle trim and static stability data for any desired flight condition. Common predefined flight conditions are included. The predefined flight conditions include six horizontal and six landing rotation conditions with varying options for engine out, crosswind and sideslip, plus three takeoff rotation conditions. Results are displayed through a unique graphical interface developed to provide stability and control information to the conceptual design engineers using a qualitative scale indicating whether the vehicle has acceptable, marginal, or unacceptable static stability characteristics. This software allows the user to prescribe the vehicle s CG location, mass, and inertia tensor so that any loading configuration between empty weight and maximum take-off weight can be analyzed. The required geometric and aerodynamic data as well as mass and inertia properties may be entered directly, passed through data files, or come from external programs such as Vehicle Sketch Pad (VSP). The current version of MASCOT has been tested with VSP used to compute the required data, which is then passed directly into the program. In VSP, the vehicle geometry is created and manipulated. The aerodynamic coefficients, stability and control derivatives, are calculated using VorLax, which is now available directly within VSP. MASCOT has been written exclusively using the technical computing language MATLAB . This innovation is able to bridge the gap between low-fidelity conceptual design and higher-fidelity stability and control analysis. This new tool enables the conceptual design engineer to include detailed static stability

  11. Spheromak tilting and its stability control

    International Nuclear Information System (INIS)

    Hayashi, T.; Sato, T.

    1983-01-01

    Spheromak tilting instability was studied. A numerical technique to create a rather arbitrarily-shaped spheromak like the one with a flux hole was investigated. The dynamics governing the tilting instability, namely, the influence of the magnetic index, the toroidal current (q-profile) and the resistivity upon the tilting growth rate, and the roles of magnetc reconnection upon the nonlinear development were studied. The best way to control the tilting instability was invented. The stabilizing effects of the vertical wall, the isolated conducting cylindrical belt, and the horizontal wall were studied. Central pole stabilization was also investigated. The influence of the wall condition, namely, whether the wall acted as a flux conserver in the spheromak creation stage or not is discussed. The present study has shown that the three- dimensional simulation is indeed useful and practical in not only studying the underlying physics but also finding a stabilization technique of spheromaks. (Kato, T.)

  12. Inter- and intra-specific pan-genomes of Borrelia burgdorferi sensu lato: genome stability and adaptive radiation

    Science.gov (United States)

    2013-01-01

    Background Lyme disease is caused by spirochete bacteria from the Borrelia burgdorferi sensu lato (B. burgdorferi s.l.) species complex. To reconstruct the evolution of B. burgdorferi s.l. and identify the genomic basis of its human virulence, we compared the genomes of 23 B. burgdorferi s.l. isolates from Europe and the United States, including B. burgdorferi sensu stricto (B. burgdorferi s.s., 14 isolates), B. afzelii (2), B. garinii (2), B. “bavariensis” (1), B. spielmanii (1), B. valaisiana (1), B. bissettii (1), and B. “finlandensis” (1). Results Robust B. burgdorferi s.s. and B. burgdorferi s.l. phylogenies were obtained using genome-wide single-nucleotide polymorphisms, despite recombination. Phylogeny-based pan-genome analysis showed that the rate of gene acquisition was higher between species than within species, suggesting adaptive speciation. Strong positive natural selection drives the sequence evolution of lipoproteins, including chromosomally-encoded genes 0102 and 0404, cp26-encoded ospC and b08, and lp54-encoded dbpA, a07, a22, a33, a53, a65. Computer simulations predicted rapid adaptive radiation of genomic groups as population size increases. Conclusions Intra- and inter-specific pan-genome sizes of B. burgdorferi s.l. expand linearly with phylogenetic diversity. Yet gene-acquisition rates in B. burgdorferi s.l. are among the lowest in bacterial pathogens, resulting in high genome stability and few lineage-specific genes. Genome adaptation of B. burgdorferi s.l. is driven predominantly by copy-number and sequence variations of lipoprotein genes. New genomic groups are likely to emerge if the current trend of B. burgdorferi s.l. population expansion continues. PMID:24112474

  13. Spider mite control and resistance management: does a genome help?

    NARCIS (Netherlands)

    Van Leeuwen, T.; Dermauw, W.; Grbic, M.; Tirry, L.; Feyereisen, R.

    2012-01-01

    The complete genome of the two-spotted spider mite, Tetranychus urticae, has been reported. This is the first sequenced genome of a highly polyphagous and resistant agricultural pest. The question as to what the genome offers the community working on spider mite control is addressed.

  14. Mutations in Cytosine-5 tRNA Methyltransferases Impact Mobile Element Expression and Genome Stability at Specific DNA Repeats

    Directory of Open Access Journals (Sweden)

    Bianca Genenncher

    2018-02-01

    Full Text Available The maintenance of eukaryotic genome stability is ensured by the interplay of transcriptional as well as post-transcriptional mechanisms that control recombination of repeat regions and the expression and mobility of transposable elements. We report here that mutations in two (cytosine-5 RNA methyltransferases, Dnmt2 and NSun2, impact the accumulation of mobile element-derived sequences and DNA repeat integrity in Drosophila. Loss of Dnmt2 function caused moderate effects under standard conditions, while heat shock exacerbated these effects. In contrast, NSun2 function affected mobile element expression and genome integrity in a heat shock-independent fashion. Reduced tRNA stability in both RCMT mutants indicated that tRNA-dependent processes affected mobile element expression and DNA repeat stability. Importantly, further experiments indicated that complex formation with RNA could also contribute to the impact of RCMT function on gene expression control. These results thus uncover a link between tRNA modification enzymes, the expression of repeat DNA, and genomic integrity.

  15. Thermal equilibrium, stability and burn control

    International Nuclear Information System (INIS)

    Cohn, D.

    1982-01-01

    A number of aspects of the thermal stability and equilibrium control of ignited tokamak plasma have been investigated. Examined approaches were passive control (the effect of radial motion, the effect of radial motion and small additional transport loss), active control (the compression and decompression of plasma, subignited operation with small amount of variable external heating, and density control), and thermal equilibrium control (additional power loss from impurity radiation and enhanced transport from increased ripple). One-D calculation has been made on thermal instability eigen-modes. It was found that for electron thermal induction loss given by Alcator scaling and for neoclassical ion transport, there was at most one unstable mode with a temperature profile which maintains the temperature profile at thermal equilibrium. The effect of the coupling of temperature fluctuation and the fluctuation in major radius was investigated. Temperature driven radial motion combined with a small amount of ripple transport loss was found to be a very effective mechanism for passive thermal stability control. (Kato, T.)

  16. Improvements in stabilizing control at Black Mountain

    International Nuclear Information System (INIS)

    Twidle, T.R.; Engelbrecht, P.C.; Koel, J.W.S.

    1986-01-01

    The process flows of the three flotation circuits at the concentrator of the Black Mountain Mineral Development Company (Pty) Ltd are described. Because of the complexity of the circuits and the associated reagent regime, the plant is equipped with a comprehensive range of control equipment. The different process-control strategies are discussed, and the modifications that have been necessary to the stabilizing control loops in the copper, lead, and zinc flotation circuits are shown in detail. It is concluded that the control strategies have continued to bring about benefits and improvements to the process. X-ray fluorescence analysis was employed in the control process. Data is also given on the accuracy of the technique

  17. Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability.

    Science.gov (United States)

    Takaki, Tohru; Montagner, Marco; Serres, Murielle P; Le Berre, Maël; Russell, Matt; Collinson, Lucy; Szuhai, Karoly; Howell, Michael; Boulton, Simon J; Sahai, Erik; Petronczki, Mark

    2017-07-24

    Altered nuclear shape is a defining feature of cancer cells. The mechanisms underlying nuclear dysmorphia in cancer remain poorly understood. Here we identify PPP1R12A and PPP1CB, two subunits of the myosin phosphatase complex that antagonizes actomyosin contractility, as proteins safeguarding nuclear integrity. Loss of PPP1R12A or PPP1CB causes nuclear fragmentation, nuclear envelope rupture, nuclear compartment breakdown and genome instability. Pharmacological or genetic inhibition of actomyosin contractility restores nuclear architecture and genome integrity in cells lacking PPP1R12A or PPP1CB. We detect actin filaments at nuclear envelope rupture sites and define the Rho-ROCK pathway as the driver of nuclear damage. Lamin A protects nuclei from the impact of actomyosin activity. Blocking contractility increases nuclear circularity in cultured cancer cells and suppresses deformations of xenograft nuclei in vivo. We conclude that actomyosin contractility is a major determinant of nuclear shape and that unrestrained contractility causes nuclear dysmorphia, nuclear envelope rupture and genome instability.

  18. Recombination-Driven Genome Evolution and Stability of Bacterial Species.

    Science.gov (United States)

    Dixit, Purushottam D; Pang, Tin Yau; Maslov, Sergei

    2017-09-01

    While bacteria divide clonally, horizontal gene transfer followed by homologous recombination is now recognized as an important contributor to their evolution. However, the details of how the competition between clonality and recombination shapes genome diversity remains poorly understood. Using a computational model, we find two principal regimes in bacterial evolution and identify two composite parameters that dictate the evolutionary fate of bacterial species. In the divergent regime, characterized by either a low recombination frequency or strict barriers to recombination, cohesion due to recombination is not sufficient to overcome the mutational drift. As a consequence, the divergence between pairs of genomes in the population steadily increases in the course of their evolution. The species lacks genetic coherence with sexually isolated clonal subpopulations continuously formed and dissolved. In contrast, in the metastable regime, characterized by a high recombination frequency combined with low barriers to recombination, genomes continuously recombine with the rest of the population. The population remains genetically cohesive and temporally stable. Notably, the transition between these two regimes can be affected by relatively small changes in evolutionary parameters. Using the Multi Locus Sequence Typing (MLST) data, we classify a number of bacterial species to be either the divergent or the metastable type. Generalizations of our framework to include selection, ecologically structured populations, and horizontal gene transfer of nonhomologous regions are discussed as well. Copyright © 2017 by the Genetics Society of America.

  19. Akt: A Double-Edged Sword in Cell Proliferation and Genome Stability

    Directory of Open Access Journals (Sweden)

    Naihan Xu

    2012-01-01

    Full Text Available The Akt family of serine/threonine protein kinases are key regulators of multiple aspects of cell behaviour, including proliferation, survival, metabolism, and tumorigenesis. Growth-factor-activated Akt signalling promotes progression through normal, unperturbed cell cycles by acting on diverse downstream factors involved in controlling the G1/S and G2/M transitions. Remarkably, several recent studies have also implicated Akt in modulating DNA damage responses and genome stability. High Akt activity can suppress ATR/Chk1 signalling and homologous recombination repair (HRR via direct phosphorylation of Chk1 or TopBP1 or, indirectly, by inhibiting recruitment of double-strand break (DSB resection factors, such as RPA, Brca1, and Rad51, to sites of damage. Loss of checkpoint and/or HRR proficiency is therefore a potential cause of genomic instability in tumor cells with high Akt. Conversely, Akt is activated by DNA double-strand breaks (DSBs in a DNA-PK- or ATM/ATR-dependent manner and in some circumstances can contribute to radioresistance by stimulating DNA repair by nonhomologous end joining (NHEJ. Akt therefore modifies both the response to and repair of genotoxic damage in complex ways that are likely to have important consequences for the therapy of tumors with deregulation of the PI3K-Akt-PTEN pathway.

  20. SPAR1/RTEL1 maintains genomic stability by suppressing homologous recombination

    Science.gov (United States)

    Barber, Louise J.; Youds, Jillian L.; Ward, Jordan D.; McIlwraith, Michael J.; O’Neil, Nigel J.; Petalcorin, Mark I.R.; Martin, Julie S.; Collis, Spencer J.; Cantor, Sharon B.; Auclair, Melissa; Tissenbaum, Heidi; West, Stephen C.; Rose, Ann M.; Boulton, Simon J.

    2013-01-01

    SUMMARY Inappropriate homologous recombination (HR) can cause gross chromosomal rearrangements that in mammalian cells may lead to tumorigenesis. In yeast, the Srs2 protein is an anti-recombinase that eliminates inappropriate recombination events, but the functional equivalent of Srs2 in higher eukaryotes has proven to be elusive. In this work, we identify C. elegans SPAR-1 as a functional analogue of Srs2 and describe its vertebrate counterpart, SPAR1/RTEL1, which is required for genome stability and tumour avoidance. We find that spar-1 mutant worms and SPAR1 knockdown human cells share characteristic phenotypes with yeast srs2 mutants, including inviability upon deletion of the sgs1/BLM homologue, hyper-recombination, and DNA damage sensitivity. In vitro, purified human SPAR1 antagonises HR by promoting the disassembly of D loop recombination intermediates in a reaction dependent upon ATP hydrolysis. We propose that loss of HR control following deregulation of SPAR1/RTEL1 may be a critical event that drives genome instability and cancer. PMID:18957201

  1. Stability of foot-and-mouth disease virus, its genome and proteins at 37 grad C

    International Nuclear Information System (INIS)

    Razdan, R.; Sen, A.K.; Rao, B.V.; Suryanarayana, V.V.S.

    1996-01-01

    Infectivity titers of foot-and-mouth disease virus (FMDV) types Asia 1 and 0 were reduced by 4 and 2 log units, respectively, after incubation at 37 grad C for 12 hours. The stability of the FMDV RNA genome at 37 grad C was studied using 32 P-labelled virus. The RNA of FMDV type 0 was found to be more stable than that of type Asia 1. Oligo(dT)-cellulose chromatography showed that 21 % and 31 % of the labelled RNA were bound to the column in the case of types Asia 1 and 0, respectively. Possible correlation between the poly(A) tail length, accessibility of the genome to nucleases and thermo-stability of the infective virus is discussed. A possible correlation between the thermo-stability of the genome and general distribution of a particular virus type seems to exist. A stable genome associated with poor virus immunogenicity may be responsible for the prevalence of FMDV type 0 in the nature. The isoelectric focussing of structural proteins isolated from the virus samples incubated at 37 grad C revealed charge differences in the major immuno-gen between the two FMDV types. A rapid proteolytic degradation of the viral immuno-gen and stability of the genome may be responsible for frequent outbreaks of FMDV, at least, in the endemic countries. (author)

  2. Genomic stability and physiological assessments of live offspring sired by a bull clone, Starbuck II.

    Science.gov (United States)

    Ortegon, H; Betts, D H; Lin, L; Coppola, G; Perrault, S D; Blondin, P; King, W A

    2007-01-01

    It appears that overt phenotypic abnormalities observed in some domestic animal clones are not transmitted to their progeny. The current study monitored Holstein heifers sired by a bull clone, Starbuck II, from weaning to puberty. Genomic stability was assessed by telomere length status and chromosomal analysis. Growth parameters, blood profiles, physical exams and reproductive parameters were assessed for 12 months (and compared to age-matched control heifers). Progeny sired by the clone bull did not differ (P>0.05) in weight, length and height compared to controls. However, progeny had lower heart rates (HR) (P=0.009), respiratory rates (RR) (P=0.007) and body temperature (P=0.03). Hematological profiles were within normal ranges and did not differ (P>0.05) between both groups. External and internal genitalia were normal and both groups reached puberty at expected ages. Progeny had two or three ovarian follicular waves per estrous cycle and serum progesterone concentrations were similar (P=0.99) to controls. Telomere lengths of sperm and blood cells from Starbuck II were not different (P>0.05) than those of non-cloned cattle; telomere lengths of progeny were not different (P>0.05) from age-matched controls. In addition, progeny had normal karyotypes in peripheral blood leukocytes compared to controls (89.1% versus 86.3% diploid, respectively). In summary, heifers sired by a bull clone had normal chromosomal stability, growth, physical, hematological and reproductive parameters, compared to normal heifers. Furthermore, they had moderate stress responses to routine handling and restraint.

  3. Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy

    DEFF Research Database (Denmark)

    Kaas, Christian Schrøder; Kristensen, Claus; Betenbaugh, Michael J.

    2015-01-01

    Background: The DHFR negative CHO DXB11 cell line (also known as DUX-B11 and DUKX) was historically the first CHO cell line to be used for large scale production of heterologous proteins and is still used for production of a number of complex proteins.  Results: Here we present the genomic sequence...... of the CHO DXB11 genome sequenced to a depth of 33x. Overall a significant genomic drift was seen favoring GC -> AT point mutations in line with the chemical mutagenesis strategy used for generation of the cell line. The sequencing depth for each gene in the genome revealed distinct peaks at sequencing...... in eight additional analyzed CHO genomes (15-20% haploidy) but not in the genome of the Chinese hamster. The dhfr gene is confirmed to be haploid in CHO DXB11; transcriptionally active and the remaining allele contains a G410C point mutation causing a Thr137Arg missense mutation. We find similar to 2...

  4. Fuzzy control. Fundamentals, stability and design of fuzzy controllers

    Energy Technology Data Exchange (ETDEWEB)

    Michels, K. [Fichtner GmbH und Co. KG, Stuttgart (Germany); Klawonn, F. [Fachhochschule Braunschweig/Wolfenbuettel (Germany). Fachbereich Informatik; Kruse, R. [Magdeburg Univ. (Germany). Fakultaet Informatik, Abt. Wiss.- und Sprachverarbeitung; Nuernberger, A. (eds.) [California Univ., Berkeley, CA (United States). Computer Science Division

    2006-07-01

    The book provides a critical discussion of fuzzy controllers from the perspective of classical control theory. Special emphases are placed on topics that are of importance for industrial applications, like (self-) tuning of fuzzy controllers, optimisation and stability analysis. The book is written as a textbook for graduate students as well as a comprehensive reference book about fuzzy control for researchers and application engineers. Starting with a detailed introduction to fuzzy systems and control theory the reader is guided to up-to-date research results. (orig.)

  5. Small cell ovarian carcinoma: genomic stability and responsiveness to therapeutics.

    Science.gov (United States)

    Gamwell, Lisa F; Gambaro, Karen; Merziotis, Maria; Crane, Colleen; Arcand, Suzanna L; Bourada, Valerie; Davis, Christopher; Squire, Jeremy A; Huntsman, David G; Tonin, Patricia N; Vanderhyden, Barbara C

    2013-02-21

    The biology of small cell ovarian carcinoma of the hypercalcemic type (SCCOHT), which is a rare and aggressive form of ovarian cancer, is poorly understood. Tumourigenicity, in vitro growth characteristics, genetic and genomic anomalies, and sensitivity to standard and novel chemotherapeutic treatments were investigated in the unique SCCOHT cell line, BIN-67, to provide further insight in the biology of this rare type of ovarian cancer. The tumourigenic potential of BIN-67 cells was determined and the tumours formed in a xenograft model was compared to human SCCOHT. DNA sequencing, spectral karyotyping and high density SNP array analysis was performed. The sensitivity of the BIN-67 cells to standard chemotherapeutic agents and to vesicular stomatitis virus (VSV) and the JX-594 vaccinia virus was tested. BIN-67 cells were capable of forming spheroids in hanging drop cultures. When xenografted into immunodeficient mice, BIN-67 cells developed into tumours that reflected the hypercalcemia and histology of human SCCOHT, notably intense expression of WT-1 and vimentin, and lack of expression of inhibin. Somatic mutations in TP53 and the most common activating mutations in KRAS and BRAF were not found in BIN-67 cells by DNA sequencing. Spectral karyotyping revealed a largely normal diploid karyotype (in greater than 95% of cells) with a visibly shorter chromosome 20 contig. High density SNP array analysis also revealed few genomic anomalies in BIN-67 cells, which included loss of heterozygosity of an estimated 16.7 Mb interval on chromosome 20. SNP array analyses of four SCCOHT samples also indicated a low frequency of genomic anomalies in the majority of cases. Although resistant to platinum chemotherapeutic drugs, BIN-67 cell viability in vitro was reduced by > 75% after infection with oncolytic viruses. These results show that SCCOHT differs from high-grade serous carcinomas by exhibiting few chromosomal anomalies and lacking TP53 mutations. Although BIN-67 cells are

  6. The Csr system regulates genome-wide mRNA stability and transcription and thus gene expression in Escherichia coli.

    Science.gov (United States)

    Esquerré, Thomas; Bouvier, Marie; Turlan, Catherine; Carpousis, Agamemnon J; Girbal, Laurence; Cocaign-Bousquet, Muriel

    2016-04-26

    Bacterial adaptation requires large-scale regulation of gene expression. We have performed a genome-wide analysis of the Csr system, which regulates many important cellular functions. The Csr system is involved in post-transcriptional regulation, but a role in transcriptional regulation has also been suggested. Two proteins, an RNA-binding protein CsrA and an atypical signaling protein CsrD, participate in the Csr system. Genome-wide transcript stabilities and levels were compared in wildtype E. coli (MG1655) and isogenic mutant strains deficient in CsrA or CsrD activity demonstrating for the first time that CsrA and CsrD are global negative and positive regulators of transcription, respectively. The role of CsrA in transcription regulation may be indirect due to the 4.6-fold increase in csrD mRNA concentration in the CsrA deficient strain. Transcriptional action of CsrA and CsrD on a few genes was validated by transcriptional fusions. In addition to an effect on transcription, CsrA stabilizes thousands of mRNAs. This is the first demonstration that CsrA is a global positive regulator of mRNA stability. For one hundred genes, we predict that direct control of mRNA stability by CsrA might contribute to metabolic adaptation by regulating expression of genes involved in carbon metabolism and transport independently of transcriptional regulation.

  7. Impact of UV Radiation on Genome Stability and Human Health.

    Science.gov (United States)

    Roy, Sujit

    2017-01-01

    Gradual depletion of the atmospheric ozone layer during the past few years has increased the incidence of solar UV radiation specifically the UV-C on earth's surface is one of the major environmental concerns because of the harmful effects of this radiation in all forms of life. The solar UV radiation including the harmful wavelength range of UV-B (280-320 nm) represents a significant climatic stress for both animals and plants, causing damage to the fundamental biomolecules such as DNA, proteins and lipids, thus activating genotoxic stress and induces genome instability. When DNA absorbs UV-B light, energy from the photon causes covalent linkages to form between adjacent pyrimidine bases, creating photoproducts, primarily cyclobutane pyrimidine dimers (CPDs) and pyrimidine-6,4-pyrimidinone photoproduct (6,4PPs). Pyrimidine dimers create distortions in the DNA strands and therefore can inhibit DNA replication as well transcription. Lack of efficient repair of UV-induced DNA damage may induce the formation of DNA double stand breaks (DSBs), one of the serious forms of damage in DNA double helix, as well as oxidative damage. Unrepaired DSBs in the actively dividing somatic cells severely affect cell growth and development, finally results in loss of cell viability and development of various diseases, such as cancer in man.This chapter mainly highlights the incidence of solar UV-radiation on earth's surface along with the formation of major types of UV-induced DNA damage and the associated repair mechanisms as well as methods of detecting DNA damage and finally our present understanding on the impact on solar UV radiation on human health.

  8. ARTEMIS stabilizes the genome and modulates proliferative responses in multipotent mesenchymal cells

    Directory of Open Access Journals (Sweden)

    Tompkins Kathleen

    2010-10-01

    Full Text Available Abstract Background Unrepaired DNA double-stranded breaks (DSBs cause chromosomal rearrangements, loss of genetic information, neoplastic transformation or cell death. The nonhomologous end joining (NHEJ pathway, catalyzing sequence-independent direct rejoining of DSBs, is a crucial mechanism for repairing both stochastically occurring and developmentally programmed DSBs. In lymphocytes, NHEJ is critical for both development and genome stability. NHEJ defects lead to severe combined immunodeficiency (SCID and lymphoid cancer predisposition in both mice and humans. While NHEJ has been thoroughly investigated in lymphocytes, the importance of NHEJ in other cell types, especially with regard to tumor suppression, is less well documented. We previously reported evidence that the NHEJ pathway functions to suppress a range of nonlymphoid tumor types, including various classes of sarcomas, by unknown mechanisms. Results Here we investigate roles for the NHEJ factor ARTEMIS in multipotent mesenchymal stem/progenitor cells (MSCs, as putative sarcomagenic cells of origin. We demonstrate a key role for ARTEMIS in sarcoma suppression in a sensitized mouse tumor model. In this context, we found that ARTEMIS deficiency led to chromosomal damage but, paradoxically, enhanced resistance and proliferative potential in primary MSCs subjected to various stresses. Gene expression analysis revealed abnormally regulated stress response, cell proliferation, and signal transduction pathways in ARTEMIS-defective MSCs. Finally, we identified candidate regulatory genes that may, in part, mediate a stress-resistant, hyperproliferative phenotype in preneoplastic ARTEMIS-deficient MSCs. Conclusions Our discoveries suggest that Art prevents genome damage and restrains proliferation in MSCs exposed to various stress stimuli. We propose that deficiency leads to a preneoplastic state in primary MSCs and is associated with aberrant proliferative control and cellular stress

  9. RECG maintains plastid and mitochondrial genome stability by suppressing extensive recombination between short dispersed repeats.

    Directory of Open Access Journals (Sweden)

    Masaki Odahara

    2015-03-01

    Full Text Available Maintenance of plastid and mitochondrial genome stability is crucial for photosynthesis and respiration, respectively. Recently, we have reported that RECA1 maintains mitochondrial genome stability by suppressing gross rearrangements induced by aberrant recombination between short dispersed repeats in the moss Physcomitrella patens. In this study, we studied a newly identified P. patens homolog of bacterial RecG helicase, RECG, some of which is localized in both plastid and mitochondrial nucleoids. RECG partially complements recG deficiency in Escherichia coli cells. A knockout (KO mutation of RECG caused characteristic phenotypes including growth delay and developmental and mitochondrial defects, which are similar to those of the RECA1 KO mutant. The RECG KO cells showed heterogeneity in these phenotypes. Analyses of RECG KO plants showed that mitochondrial genome was destabilized due to a recombination between 8-79 bp repeats and the pattern of the recombination partly differed from that observed in the RECA1 KO mutants. The mitochondrial DNA (mtDNA instability was greater in severe phenotypic RECG KO cells than that in mild phenotypic ones. This result suggests that mitochondrial genomic instability is responsible for the defective phenotypes of RECG KO plants. Some of the induced recombination caused efficient genomic rearrangements in RECG KO mitochondria. Such loci were sometimes associated with a decrease in the levels of normal mtDNA and significant decrease in the number of transcripts derived from the loci. In addition, the RECG KO mutation caused remarkable plastid abnormalities and induced recombination between short repeats (12-63 bp in the plastid DNA. These results suggest that RECG plays a role in the maintenance of both plastid and mitochondrial genome stability by suppressing aberrant recombination between dispersed short repeats; this role is crucial for plastid and mitochondrial functions.

  10. STUDY ABOUT THE STABILITY AND CONTROL OF A ROTOR AIRPLANE

    OpenAIRE

    Stafy, Victor; Neto, Aristeu Silveira

    2017-01-01

    On this paper is studied the stability and control of a Rotor Airplane, more specifically a MAV (Micro Air Vehicle) and how works the dynamics of flight of this unusual configuration of aircraft. It’s discussed the impact of the gyroscopic effect on stability (mainly lateral and directional stability) and was found the best feasible configuration of the stability surface, but the project of the stabilizers was limited to lifting surfaces that use airfoil sections, the possibility of a very un...

  11. Genome Stability of Lyme Disease Spirochetes: Comparative Genomics of Borrelia burgdorferi Plasmids

    Energy Technology Data Exchange (ETDEWEB)

    Casjens S. R.; Dunn J.; Mongodin, E. F.; Qiu, W.-G.; Luft, B. J.; Schutzer, S. E.; Gilcrease, E. B.; Huang, W. M.; Vujadinovic, M.; Aron, J. K.; Vargas, L. C.; Freeman, S.; Radune, D.; Weidman, J. F.; Dimitrov, G. I.; Khouri, H. M.; Sosa, J. E.; Halpin, R. A.; Fraser, C. M.

    2012-03-14

    Lyme disease is the most common tick-borne human illness in North America. In order to understand the molecular pathogenesis, natural diversity, population structure and epizootic spread of the North American Lyme agent, Borrelia burgdorferi sensu stricto, a much better understanding of the natural diversity of its genome will be required. Towards this end we present a comparative analysis of the nucleotide sequences of the numerous plasmids of B. burgdorferi isolates B31, N40, JD1 and 297. These strains were chosen because they include the three most commonly studied laboratory strains, and because they represent different major genetic lineages and so are informative regarding the genetic diversity and evolution of this organism. A unique feature of Borrelia genomes is that they carry a large number of linear and circular plasmids, and this work shows that strains N40, JD1, 297 and B31 carry related but non-identical sets of 16, 20, 19 and 21 plasmids, respectively, that comprise 33-40% of their genomes. We deduce that there are at least 28 plasmid compatibility types among the four strains. The B. burgdorferi {approx}900 Kbp linear chromosomes are evolutionarily exceptionally stable, except for a short {le}20 Kbp plasmid-like section at the right end. A few of the plasmids, including the linear lp54 and circular cp26, are also very stable. We show here that the other plasmids, especially the linear ones, are considerably more variable. Nearly all of the linear plasmids have undergone one or more substantial inter-plasmid rearrangements since their last common ancestor. In spite of these rearrangements and differences in plasmid contents, the overall gene complement of the different isolates has remained relatively constant.

  12. Passivity-Based Control for Two-Wheeled Robot Stabilization

    Science.gov (United States)

    Uddin, Nur; Aryo Nugroho, Teguh; Agung Pramudito, Wahyu

    2018-04-01

    A passivity-based control system design for two-wheeled robot (TWR) stabilization is presented. A TWR is a statically-unstable non-linear system. A control system is applied to actively stabilize the TWR. Passivity-based control method is applied to design the control system. The design results in a state feedback control law that makes the TWR closed loop system globally asymptotically stable (GAS). The GAS is proven mathematically. The TWR stabilization is demonstrated through computer simulation. The simulation results show that the designed control system is able to stabilize the TWR.

  13. Optimal control theory applied to fusion plasma thermal stabilization

    International Nuclear Information System (INIS)

    Sager, G.; Miley, G.; Maya, I.

    1985-01-01

    Many authors have investigated stability characteristics and performance of various burn control schemes. The work presented here represents the first application of optimal control theory to the problem of fusion plasma thermal stabilization. The objectives of this initial investigation were to develop analysis methods, demonstrate tractability, and present some preliminary results of optimal control theory in burn control research

  14. LHC beam stability and feedback control

    Energy Technology Data Exchange (ETDEWEB)

    Steinhagen, Ralph

    2007-07-20

    This report presents the stability and the control of the Large Hadron Collider's (LHC) two beam orbits and their particle momenta using beam-based feedback systems. The aim of this report is to contribute to a safe and reliable LHC commissioning and machine operation. The first part of the analysis gives an estimate of the expected sources of orbit and energy perturbations that can be grouped into environmental sources, machine-inherent sources and machine element failures: the slowest perturbation due to ground motion, tides, temperature fluctuations of the tunnel and other environmental influences are described in this report by a propagation model that is both qualitatively and quantitatively supported by geophone and beam motion measurements at LEP and other CERN accelerators. The second part of this analysis deals with the control of the two LHC beams' orbit and energy through automated feedback systems. Based on the reading of the more than 1056 beam position monitors (BPMs) that are distributed over the machine, a central global feedback controller calculates new deflection strengths for the more than 1060 orbit corrector magnets (CODs) that are suitable to correct the orbit and momentum around their references. this report provides an analysis of the BPMs and CODs involved in the orbit and energy feedback. The BPMs are based on a wide-band time normaliser circuit that converts the transverse beam position reading of each individual particle bunch into two laser pulses that are separated by a time delay and transmitted through optical fibres to an acquisition card that converts the delay signals into a digital position. A simple error model has been tested and compared to the measurement accuracy of LHC type BPMs, obtained through beam-based measurements in the SPS. The average beam position is controlled through 1060 superconducting and individually powered corrector dipole magnets. The proposed correction in 'time-domain' consists of a

  15. LHC beam stability and feedback control

    International Nuclear Information System (INIS)

    Steinhagen, Ralph

    2007-01-01

    This report presents the stability and the control of the Large Hadron Collider's (LHC) two beam orbits and their particle momenta using beam-based feedback systems. The aim of this report is to contribute to a safe and reliable LHC commissioning and machine operation. The first part of the analysis gives an estimate of the expected sources of orbit and energy perturbations that can be grouped into environmental sources, machine-inherent sources and machine element failures: the slowest perturbation due to ground motion, tides, temperature fluctuations of the tunnel and other environmental influences are described in this report by a propagation model that is both qualitatively and quantitatively supported by geophone and beam motion measurements at LEP and other CERN accelerators. The second part of this analysis deals with the control of the two LHC beams' orbit and energy through automated feedback systems. Based on the reading of the more than 1056 beam position monitors (BPMs) that are distributed over the machine, a central global feedback controller calculates new deflection strengths for the more than 1060 orbit corrector magnets (CODs) that are suitable to correct the orbit and momentum around their references. this report provides an analysis of the BPMs and CODs involved in the orbit and energy feedback. The BPMs are based on a wide-band time normaliser circuit that converts the transverse beam position reading of each individual particle bunch into two laser pulses that are separated by a time delay and transmitted through optical fibres to an acquisition card that converts the delay signals into a digital position. A simple error model has been tested and compared to the measurement accuracy of LHC type BPMs, obtained through beam-based measurements in the SPS. The average beam position is controlled through 1060 superconducting and individually powered corrector dipole magnets. The proposed correction in 'time-domain' consists of a proportional

  16. The role of the Fanconi anemia pathway in DNA repair and maintenance of genome stability

    Directory of Open Access Journals (Sweden)

    Aleksandra M. Koczorowska

    2014-05-01

    Full Text Available The Fanconi anemia (FA pathway is one of the DNA repair systems involved in removal of DNA crosslinks. Proteins which belong to this pathway are crucial to the protection of genetic information, whereas disturbances in their function have serious implications for the whole organism. Biallelic mutations in FA genes are the cause of Fanconi anemia – a genetic disease which manifests itself through numerous congenital abnormalities, chromosomal instability and increased predisposition to cancer. The FA pathway is composed of fifteen proteins. Eight of them, in the presence of DNA interstrand crosslinks (ICLs, form a nuclear core complex responsible for monoubiquitination of FANCD2 and FANCI, which is a key step of ICL repair. FA proteins which are not involved in the monoubiquitination step participate in repair of DNA double strand breaks via homologous recombination. Some of the FA proteins, besides having a direct role in the repair of DNA damage, are engaged in replication, cell cycle control and mitosis. The unperturbed course of those processes determines the maintenance of genome stability.

  17. Maintaining Genome Stability in Defiance of Mitotic DNA Damage

    Science.gov (United States)

    Ferrari, Stefano; Gentili, Christian

    2016-01-01

    The implementation of decisions affecting cell viability and proliferation is based on prompt detection of the issue to be addressed, formulation and transmission of a correct set of instructions and fidelity in the execution of orders. While the first and the last are purely mechanical processes relying on the faithful functioning of single proteins or macromolecular complexes (sensors and effectors), information is the real cue, with signal amplitude, duration, and frequency ultimately determining the type of response. The cellular response to DNA damage is no exception to the rule. In this review article we focus on DNA damage responses in G2 and Mitosis. First, we set the stage describing mitosis and the machineries in charge of assembling the apparatus responsible for chromosome alignment and segregation as well as the inputs that control its function (checkpoints). Next, we examine the type of issues that a cell approaching mitosis might face, presenting the impact of post-translational modifications (PTMs) on the correct and timely functioning of pathways correcting errors or damage before chromosome segregation. We conclude this essay with a perspective on the current status of mitotic signaling pathway inhibitors and their potential use in cancer therapy. PMID:27493659

  18. Replicative Stress and the FHIT Gene: Roles in Tumor Suppression, Genome Stability and Prevention of Carcinogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Karras, Jenna R.; Paisie, Carolyn A.; Huebner, Kay, E-mail: kay.huebner@osumc.edu [Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University Wexner Medical Center, Columbus, OH 43210 (United States)

    2014-06-04

    The fragile FHIT gene, encompassing the chromosomal fragile site FRA3B, is an early target of DNA damage in precancerous cells. While vulnerable to DNA damage itself, FHIT protein expression is essential to protect from DNA damage-induced cancer initiation and progression by modulating genome stability, oxidative stress and levels of accumulating DNA damage. Thus, FHIT, whose expression is lost or reduced in many human cancers, is a tumor suppressor and genome caretaker whose loss initiates genome instability in preneoplastic lesions. Ongoing studies are seeking more detailed understanding of the role of FHIT in the cellular response to oxidative damage. This review discusses the relationship between FHIT, reactive oxygen species production, and DNA damage in the context of cancer initiation and progression.

  19. The human RecQ helicases BLM and RECQL4 cooperate to preserve genome stability

    Czech Academy of Sciences Publication Activity Database

    Singh, D.K.; Popuri, V.; Kulikowicz, T.; Shevelev, Igor; Ghosh, A.K.; Ramamoorthy, M.; Rossi, M.L.; Janščák, Pavel; Croteau, D.L.; Bohr, V.A.

    2012-01-01

    Roč. 40, č. 14 (2012), s. 6632-6648 ISSN 0305-1048 R&D Projects: GA ČR GAP305/10/0281 Grant - others:NIH(US) Z01-AG000726-17 Institutional research plan: CEZ:AV0Z50520514 Institutional support: RVO:68378050 Keywords : RecQ helicase * genome stability * BLM * RECQL4 Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 8.278, year: 2012

  20. The C-terminal domain of the Bloom syndrome DNA helicase is essential for genomic stability

    Directory of Open Access Journals (Sweden)

    Noonan James P

    2001-07-01

    Full Text Available Abstract Background Bloom syndrome is a rare cancer-prone disorder in which the cells of affected persons have a high frequency of somatic mutation and genomic instability. Bloom syndrome cells have a distinctive high frequency of sister chromatid exchange and quadriradial formation. BLM, the protein altered in BS, is a member of the RecQ DNA helicase family, whose members share an average of 40% identity in the helicase domain and have divergent N-terminal and C-terminal flanking regions of variable lengths. The BLM DNA helicase has been shown to localize to the ND10 (nuclear domain 10 or PML (promyelocytic leukemia nuclear bodies, where it associates with TOPIIIα, and to the nucleolus. Results This report demonstrates that the N-terminal domain of BLM is responsible for localization of the protein to the nuclear bodies, while the C-terminal domain directs the protein to the nucleolus. Deletions of the N-terminal domain of BLM have little effect on sister chromatid exchange frequency and chromosome stability as compared to helicase and C-terminal mutations which can increase SCE frequency and chromosome abnormalities. Conclusion The helicase activity and the C-terminal domain of BLM are critical for maintaining genomic stability as measured by the sister chromatid exchange assay. The localization of BLM into the nucleolus by the C-terminal domain appears to be more important to genomic stability than localization in the nuclear bodies.

  1. Nonlinear stability control and λ-bifurcation

    International Nuclear Information System (INIS)

    Erneux, T.; Reiss, E.L.; Magnan, J.F.; Jayakumar, P.K.

    1987-01-01

    Passive techniques for nonlinear stability control are presented for a model of fluidelastic instability. They employ the phenomena of λ-bifurcation and a generalization of it. λ-bifurcation occurs when a branch of flutter solutions bifurcates supercritically from a basic solution and terminates with an infinite period orbit at a branch of divergence solutions which bifurcates subcritically from the basic solution. The shape of the bifurcation diagram then resembles the greek letter λ. When the system parameters are in the range where flutter occurs by λ-bifurcation, then as the flow velocity increase the flutter amplitude also increases, but the frequencies of the oscillations decrease to zero. This diminishes the damaging effects of structural fatigue by flutter, and permits the flow speed to exceed the critical flutter speed. If generalized λ-bifurcation occurs, then there is a jump transition from the flutter states to a divergence state with a substantially smaller amplitude, when the flow speed is sufficiently larger than the critical flutter speed

  2. Stabilization of model-based networked control systems

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, Francisco [CIDMA, Universidade de Aveiro, Aveiro (Portugal); Instituto Politécnico de Viana do Castelo, Viana do Castelo (Portugal); Abreu, Carlos [Instituto Politécnico de Viana do Castelo, Viana do Castelo (Portugal); CMEMS-UMINHO, Universidade do Minho, Braga (Portugal); Mendes, Paulo M. [CMEMS-UMINHO, Universidade do Minho, Braga (Portugal)

    2016-06-08

    A class of networked control systems called Model-Based Networked Control Systems (MB-NCSs) is considered. Stabilization of MB-NCSs is studied using feedback controls and simulation of stabilization for different feedbacks is made with the purpose to reduce the network trafic. The feedback control input is applied in a compensated model of the plant that approximates the plant dynamics and stabilizes the plant even under slow network conditions. Conditions for global exponential stabilizability and for the choosing of a feedback control input for a given constant time between the information moments of the network are derived. An optimal control problem to obtain an optimal feedback control is also presented.

  3. New tools for the stability control

    International Nuclear Information System (INIS)

    Melera, J.; Albendea, M.; Escriva, A.; Munoz-Cobos, L. L.; Montesinos

    2006-01-01

    IBERDROLA INGENIERIA y CONSTRUCCION, IBERDROLA GENERACION and the polytechnical Univisty of Valencia, are carrying out a project of R and D called DROP (Decay Ratio on-line Predictor) whose objective is to create a tool (predictor and monitor) for on-line monitoring of the thermal-hydraulic stability during the operation of BWR reactors. DROP is intended to be integrated in CAPRICOPE; the Core Monitoring System of Cofrentes NPP, to allow supervisors to known stability margins during the operation, simplifying reactor start-up and shutdown. In transient conditions, the knowledge of reactor stability will avoid reactor unplanned shutdowns. (Author) 11 refs

  4. Emissions Control in Swirl-Stabilized Combustors

    National Research Council Canada - National Science Library

    Hanson, Ronald K

    2006-01-01

    ...) fabricate a swirl-stabilized gas and liquid fuel burner with optical access to enable diagnostic development that mimics the atmospheric pressure performance of the University of Cincinnati facility, and 4...

  5. Computation of stabilizing PI and PID controllers using the stability boundary locus

    International Nuclear Information System (INIS)

    Tan, Nusret; Kaya, Ibrahim; Yeroglu, Celaleddin; Atherton, Derek P.

    2006-01-01

    In this paper, a new method for the calculation of all stabilizing PI controllers is given. The proposed method is based on plotting the stability boundary locus in the (k p , k i )-plane and then computing the stabilizing values of the parameters of a PI controller. The technique presented does not require sweeping over the parameters and also does not need linear programming to solve a set of inequalities. Thus it offers several important advantages over existing results obtained in this direction. Beyond stabilization, the method is used to shift all poles to a shifted half plane that guarantees a specified settling time of response. Computation of stabilizing PI controllers which achieve user specified gain and phase margins is studied. It is shown via an example that the stabilizing region in the (k p , k i )-plane is not always a convex set. The proposed method is also used to design PID controllers. The limiting values of a PID controller which stabilize a given system are obtained in the (k p , k i )-plane (k p , k d )-plane and (k i , k d )-plane. Furthermore, the proposed method is used to compute all the parameters of a PI controller which stabilize a control system with an interval plant family. Examples are given to show the benefits of the method presented

  6. Computation of robustly stabilizing PID controllers for interval systems.

    Science.gov (United States)

    Matušů, Radek; Prokop, Roman

    2016-01-01

    The paper is focused on the computation of all possible robustly stabilizing Proportional-Integral-Derivative (PID) controllers for plants with interval uncertainty. The main idea of the proposed method is based on Tan's (et al.) technique for calculation of (nominally) stabilizing PI and PID controllers or robustly stabilizing PI controllers by means of plotting the stability boundary locus in either P-I plane or P-I-D space. Refinement of the existing method by consideration of 16 segment plants instead of 16 Kharitonov plants provides an elegant and efficient tool for finding all robustly stabilizing PID controllers for an interval system. The validity and relatively effortless application of presented theoretical concepts are demonstrated through a computation and simulation example in which the uncertain mathematical model of an experimental oblique wing aircraft is robustly stabilized.

  7. From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond.

    Science.gov (United States)

    Michelini, Flavia; Jalihal, Ameya P; Francia, Sofia; Meers, Chance; Neeb, Zachary T; Rossiello, Francesca; Gioia, Ubaldo; Aguado, Julio; Jones-Weinert, Corey; Luke, Brian; Biamonti, Giuseppe; Nowacki, Mariusz; Storici, Francesca; Carninci, Piero; Walter, Nils G; Fagagna, Fabrizio d'Adda di

    2018-03-30

    Coding for proteins has been considered the main function of RNA since the "central dogma" of biology was proposed. The discovery of noncoding transcripts shed light on additional roles of RNA, ranging from the support of polypeptide synthesis, to the assembly of subnuclear structures, to gene expression modulation. Cellular RNA has therefore been recognized as a central player in often unanticipated biological processes, including genomic stability. This ever-expanding list of functions inspired us to think of RNA as a "smart" phone, which has replaced the older obsolete "cellular" phone. In this review, we summarize the last two decades of advances in research on the interface between RNA biology and genome stability. We start with an account of the emergence of noncoding RNA, and then we discuss the involvement of RNA in DNA damage signaling and repair, telomere maintenance, and genomic rearrangements. We continue with the depiction of single-molecule RNA detection techniques, and we conclude by illustrating the possibilities of RNA modulation in hopes of creating or improving new therapies. The widespread biological functions of RNA have made this molecule a reoccurring theme in basic and translational research, warranting it the transcendence from classically studied "cellular" RNA to "smart" RNA.

  8. The influence of micronutrients in cell culture: a reflection on viability and genomic stability.

    Science.gov (United States)

    Arigony, Ana Lúcia Vargas; de Oliveira, Iuri Marques; Machado, Miriana; Bordin, Diana Lilian; Bergter, Lothar; Prá, Daniel; Henriques, João Antonio Pêgas

    2013-01-01

    Micronutrients, including minerals and vitamins, are indispensable to DNA metabolic pathways and thus are as important for life as macronutrients. Without the proper nutrients, genomic instability compromises homeostasis, leading to chronic diseases and certain types of cancer. Cell-culture media try to mimic the in vivo environment, providing in vitro models used to infer cells' responses to different stimuli. This review summarizes and discusses studies of cell-culture supplementation with micronutrients that can increase cell viability and genomic stability, with a particular focus on previous in vitro experiments. In these studies, the cell-culture media include certain vitamins and minerals at concentrations not equal to the physiological levels. In many common culture media, the sole source of micronutrients is fetal bovine serum (FBS), which contributes to only 5-10% of the media composition. Minimal attention has been dedicated to FBS composition, micronutrients in cell cultures as a whole, or the influence of micronutrients on the viability and genetics of cultured cells. Further studies better evaluating micronutrients' roles at a molecular level and influence on the genomic stability of cells are still needed.

  9. Asymptotic stability of a genetic network under impulsive control

    International Nuclear Information System (INIS)

    Li Fangfei; Sun Jitao

    2010-01-01

    The study of the stability of genetic network is an important motif for the understanding of the living organism at both molecular and cellular levels. In this Letter, we provide a theoretical method for analyzing the asymptotic stability of a genetic network under impulsive control. And the sufficient conditions of its asymptotic stability under impulsive control are obtained. Finally, an example is given to illustrate the effectiveness of the obtained method.

  10. Power system stabilizers based on modern control techniques

    Energy Technology Data Exchange (ETDEWEB)

    Malik, O P; Chen, G P; Zhang, Y; El-Metwally, K [Calgary Univ., AB (Canada). Dept. of Electrical and Computer Engineering

    1994-12-31

    Developments in digital technology have made it feasible to develop and implement improved controllers based on sophisticated control techniques. Power system stabilizers based on adaptive control, fuzzy logic and artificial networks are being developed. Each of these control techniques possesses unique features and strengths. In this paper, the relative performance of power systems stabilizers based on adaptive control, fuzzy logic and neural network, both in simulation studies and real time tests on a physical model of a power system, is presented and compared to that of a fixed parameter conventional power system stabilizer. (author) 16 refs., 45 figs., 3 tabs.

  11. Stabilization of Networked Control Systems Under Feedback-based Communication

    National Research Council Canada - National Science Library

    Zhang, Lei; Hristu-Varsakelis, Dimitrios

    2004-01-01

    We study the stabilization of a networked control system (NSC) in which multiple sensors and actuators of a physical plant share a communication medium to exchange information with a remote controller...

  12. Impact of nuclear organization and chromatin structure on DNA repair and genome stability

    International Nuclear Information System (INIS)

    Batte, Amandine

    2016-01-01

    The non-random organization of the eukaryotic cell nucleus and the folding of genome in chromatin more or less condensed can influence many functions related to DNA metabolism, including genome stability. Double-strand breaks (DSBs) are the most deleterious DNA damages for the cells. To preserve genome integrity, eukaryotic cells thus developed DSB repair mechanisms conserved from yeast to human, among which homologous recombination (HR) that uses an intact homologous sequence to repair a broken chromosome. HR can be separated in two sub-pathways: Gene Conversion (GC) transfers genetic information from one molecule to its homologous and Break Induced Replication (BIR) establishes a replication fork than can proceed until the chromosome end. My doctorate work was focused on the contribution of the chromatin context and 3D genome organization on DSB repair. In S. cerevisiae, nuclear organization and heterochromatin spreading at sub-telomeres can be modified through the overexpression of the Sir3 or sir3A2Q mutant proteins. We demonstrated that reducing the physical distance between homologous sequences increased GC rates, reinforcing the notion that homology search is a limiting step for recombination. We also showed that hetero-chromatinization of DSB site fine-tunes DSB resection, limiting the loss of the DSB ends required to perform homology search and complete HR. Finally, we noticed that the presence of heterochromatin at the donor locus decreased both GC and BIR efficiencies, probably by affecting strand invasion. This work highlights new regulatory pathways of DNA repair. (author) [fr

  13. Multifunctional Role of ATM/Tel1 Kinase in Genome Stability: From the DNA Damage Response to Telomere Maintenance

    Science.gov (United States)

    2014-01-01

    The mammalian protein kinase ataxia telangiectasia mutated (ATM) is a key regulator of the DNA double-strand-break response and belongs to the evolutionary conserved phosphatidylinositol-3-kinase-related protein kinases. ATM deficiency causes ataxia telangiectasia (AT), a genetic disorder that is characterized by premature aging, cerebellar neuropathy, immunodeficiency, and predisposition to cancer. AT cells show defects in the DNA damage-response pathway, cell-cycle control, and telomere maintenance and length regulation. Likewise, in Saccharomyces cerevisiae, haploid strains defective in the TEL1 gene, the ATM ortholog, show chromosomal aberrations and short telomeres. In this review, we outline the complex role of ATM/Tel1 in maintaining genomic stability through its control of numerous aspects of cellular survival. In particular, we describe how ATM/Tel1 participates in the signal transduction pathways elicited by DNA damage and in telomere homeostasis and its importance as a barrier to cancer development. PMID:25247188

  14. DOT1L and H3K79 Methylation in Transcription and Genomic Stability.

    Science.gov (United States)

    Wood, Katherine; Tellier, Michael; Murphy, Shona

    2018-02-27

    The organization of eukaryotic genomes into chromatin provides challenges for the cell to accomplish basic cellular functions, such as transcription, DNA replication and repair of DNA damage. Accordingly, a range of proteins modify and/or read chromatin states to regulate access to chromosomal DNA. Yeast Dot1 and the mammalian homologue DOT1L are methyltransferases that can add up to three methyl groups to histone H3 lysine 79 (H3K79). H3K79 methylation is implicated in several processes, including transcription elongation by RNA polymerase II, the DNA damage response and cell cycle checkpoint activation. DOT1L is also an important drug target for treatment of mixed lineage leukemia (MLL)-rearranged leukemia where aberrant transcriptional activation is promoted by DOT1L mislocalisation. This review summarizes what is currently known about the role of Dot1/DOT1L and H3K79 methylation in transcription and genomic stability.

  15. DOT1L and H3K79 Methylation in Transcription and Genomic Stability

    Directory of Open Access Journals (Sweden)

    Katherine Wood

    2018-02-01

    Full Text Available The organization of eukaryotic genomes into chromatin provides challenges for the cell to accomplish basic cellular functions, such as transcription, DNA replication and repair of DNA damage. Accordingly, a range of proteins modify and/or read chromatin states to regulate access to chromosomal DNA. Yeast Dot1 and the mammalian homologue DOT1L are methyltransferases that can add up to three methyl groups to histone H3 lysine 79 (H3K79. H3K79 methylation is implicated in several processes, including transcription elongation by RNA polymerase II, the DNA damage response and cell cycle checkpoint activation. DOT1L is also an important drug target for treatment of mixed lineage leukemia (MLL-rearranged leukemia where aberrant transcriptional activation is promoted by DOT1L mislocalisation. This review summarizes what is currently known about the role of Dot1/DOT1L and H3K79 methylation in transcription and genomic stability.

  16. Coaxial jets stability and control possibility

    Czech Academy of Sciences Publication Activity Database

    Trávníček, Zdeněk; Hrubý, Jan; Vogel, Jiří

    2005-01-01

    Roč. 4, - (2005), s. 121-130. ISBN 83-89334-82-8 R&D Projects: GA AV ČR(CZ) IAA2076203 Institutional research plan: CEZ:AV0Z20760514 Keywords : jets * stability * visualization Subject RIV: BK - Fluid Dynamics

  17. Local chromatin structure of heterochromatin regulates repeated DNA stability, nucleolus structure, and genome integrity

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Jamy C. [Univ. of California, Berkeley, CA (United States)

    2007-01-01

    Heterochromatin constitutes a significant portion of the genome in higher eukaryotes; approximately 30% in Drosophila and human. Heterochromatin contains a high repeat DNA content and a low density of protein-encoding genes. In contrast, euchromatin is composed mostly of unique sequences and contains the majority of single-copy genes. Genetic and cytological studies demonstrated that heterochromatin exhibits regulatory roles in chromosome organization, centromere function and telomere protection. As an epigenetically regulated structure, heterochromatin formation is not defined by any DNA sequence consensus. Heterochromatin is characterized by its association with nucleosomes containing methylated-lysine 9 of histone H3 (H3K9me), heterochromatin protein 1 (HP1) that binds H3K9me, and Su(var)3-9, which methylates H3K9 and binds HP1. Heterochromatin formation and functions are influenced by HP1, Su(var)3-9, and the RNA interference (RNAi) pathway. My thesis project investigates how heterochromatin formation and function impact nuclear architecture, repeated DNA organization, and genome stability in Drosophila melanogaster. H3K9me-based chromatin reduces extrachromosomal DNA formation; most likely by restricting the access of repair machineries to repeated DNAs. Reducing extrachromosomal ribosomal DNA stabilizes rDNA repeats and the nucleolus structure. H3K9me-based chromatin also inhibits DNA damage in heterochromatin. Cells with compromised heterochromatin structure, due to Su(var)3-9 or dcr-2 (a component of the RNAi pathway) mutations, display severe DNA damage in heterochromatin compared to wild type. In these mutant cells, accumulated DNA damage leads to chromosomal defects such as translocations, defective DNA repair response, and activation of the G2-M DNA repair and mitotic checkpoints that ensure cellular and animal viability. My thesis research suggests that DNA replication, repair, and recombination mechanisms in heterochromatin differ from those in

  18. Optical Materials with a Genome: Nanophotonics with DNA-Stabilized Silver Clusters

    Science.gov (United States)

    Copp, Stacy M.

    Fluorescent silver clusters with unique rod-like geometries are stabilized by DNA. The sizes and colors of these clusters, or AgN-DNA, are selected by DNA base sequence, which can tune peak emission from blue-green into the near-infrared. Combined with DNA nanostructures, AgN-DNA promise exciting applications in nanophotonics and sensing. Until recently, however, a lack of understanding of the mechanisms controlling AgN-DNA fluorescence has challenged such applications. This dissertation discusses progress toward understanding the role of DNA as a "genome" for silver clusters and toward using DNA to achieve atomic-scale precision of silver cluster size and nanometer-scale precision of silver cluster position on a DNA breadboard. We also investigate sensitivity of AgN-DNA to local solvent environment, with an eye toward applications in chemical and biochemical sensing. Using robotic techniques to generate large data sets, we show that fluorescent silver clusters are templated by certain DNA base motifs that select "magic-sized" cluster cores of enhanced stabilities. The linear arrangement of bases on the phosphate backbone imposes a unique rod-like geometry on the clusters. Harnessing machine learning and bioinformatics techniques, we also demonstrate that sequences of DNA templates can be selected to stabilize silver clusters with desired optical properties, including high fluorescence intensity and specific fluorescence wavelengths, with much higher rates of success as compared to current strategies. The discovered base motifs can be also used to design modular DNA host strands that enable individual silver clusters with atomically precise sizes to bind at specific programmed locations on a DNA nanostructure. We show that DNA-mediated nanoscale arrangement enables near-field coupling of distinct clusters, demonstrated by dual-color cluster assemblies exhibiting resonant energy transfer. These results demonstrate a new degree of control over the optical properties

  19. The algebraic criteria for the stability of control systems

    Science.gov (United States)

    Cremer, H.; Effertz, F. H.

    1986-01-01

    This paper critically examines the standard algebraic criteria for the stability of linear control systems and their proofs, reveals important previously unnoticed connections, and presents new representations. Algebraic stability criteria have also acquired significance for stability studies of non-linear differential equation systems by the Krylov-Bogoljubov-Magnus Method, and allow realization conditions to be determined for classes of broken rational functions as frequency characteristics of electrical network.

  20. Angle Stability Analysis for Voltage-Controlled Converters

    DEFF Research Database (Denmark)

    Lin, Hengwei; Jia, Chenxi; Guerrero, Josep M.

    2017-01-01

    a criterion to analyze the quasi-steady angle stability and the direct current (DC) side stability for VSCs. The operating limit and the angle instability mechanism are revealed, which is generally applicable to the voltage-controlled converters. During the analysis, the influence of the parameters on angle...... stability is studied. Further, the difference on instability mechanism between power electronic converters and synchronous generators are explained in detail. Finally, experiment results with corrective actions verify the analysis....

  1. A robust nonlinear stabilizer as a controller for improving transient stability in micro-grids.

    Science.gov (United States)

    Azimi, Seyed Mohammad; Afsharnia, Saeed

    2017-01-01

    This paper proposes a parametric-Lyapunov approach to the design of a stabilizer aimed at improving the transient stability of micro-grids (MGs). This strategy is applied to electronically-interfaced distributed resources (EI-DRs) operating with a unified control configuration applicable to all operational modes (i.e. grid-connected mode, islanded mode, and mode transitions). The proposed approach employs a simple structure compared with other nonlinear controllers, allowing ready implementation of the stabilizer. A new parametric-Lyapunov function is proposed rendering the proposed stabilizer more effective in damping system transition transients. The robustness of the proposed stabilizer is also verified based on both time-domain simulations and mathematical proofs, and an ultimate bound has been derived for the frequency transition transients. The proposed stabilizer operates by deploying solely local information and there are no needs for communication links. The deteriorating effects of the primary resource delays on the transient stability are also treated analytically. Finally, the effectiveness of the proposed stabilizer is evaluated through time-domain simulations and compared with the recently-developed stabilizers performed on a multi-resource MG. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

  2. Stabilization with guaranteed safety using Control Lyapunov–Barrier Function

    NARCIS (Netherlands)

    Romdlony, Muhammad Zakiyullah; Jayawardhana, Bayu

    2016-01-01

    We propose a novel nonlinear control method for solving the problem of stabilization with guaranteed safety for nonlinear systems. The design is based on the merging of the well-known Control Lyapunov Function (CLF) and the recent concept of Control Barrier Function (CBF). The proposed control

  3. Robust Stabilization of Nonlinear Systems with Uncertain Varying Control Coefficient

    Directory of Open Access Journals (Sweden)

    Zaiyue Yang

    2014-01-01

    Full Text Available This paper investigates the stabilization problem for a class of nonlinear systems, whose control coefficient is uncertain and varies continuously in value and sign. The study emphasizes the development of a robust control that consists of a modified Nussbaum function to tackle the uncertain varying control coefficient. By such a method, the finite-time escape phenomenon has been prevented when the control coefficient is crossing zero and varying its sign. The proposed control guarantees the asymptotic stabilization of the system and boundedness of all closed-loop signals. The control performance is illustrated by a numerical simulation.

  4. The Arabidopsis thaliana homolog of the helicase RTEL1 plays multiple roles in preserving genome stability.

    Science.gov (United States)

    Recker, Julia; Knoll, Alexander; Puchta, Holger

    2014-12-01

    In humans, mutations in the DNA helicase Regulator of Telomere Elongation Helicase1 (RTEL1) lead to Hoyeraal-Hreidarsson syndrome, a severe, multisystem disorder. Here, we demonstrate that the RTEL1 homolog in Arabidopsis thaliana plays multiple roles in preserving genome stability. RTEL1 suppresses homologous recombination in a pathway parallel to that of the DNA translocase FANCM. Cytological analyses of root meristems indicate that RTEL1 is involved in processing DNA replication intermediates independently from FANCM and the nuclease MUS81. Moreover, RTEL1 is involved in interstrand and intrastrand DNA cross-link repair independently from FANCM and (in intrastrand cross-link repair) parallel to MUS81. RTEL1 contributes to telomere homeostasis; the concurrent loss of RTEL1 and the telomerase TERT leads to rapid, severe telomere shortening, which occurs much more rapidly than it does in the single-mutant line tert, resulting in developmental arrest after four generations. The double mutant rtel1-1 recq4A-4 exhibits massive growth defects, indicating that this RecQ family helicase, which is also involved in the suppression of homologous recombination and the repair of DNA lesions, can partially replace RTEL1 in the processing of DNA intermediates. The requirement for RTEL1 in multiple pathways to preserve genome stability in plants can be explained by its putative role in the destabilization of DNA loop structures, such as D-loops and T-loops. © 2014 American Society of Plant Biologists. All rights reserved.

  5. Stabilization and trajectory tracking control for underactuated

    Indian Academy of Sciences (India)

    The control of quadrotor helicopter has been a great challenge for control engineers and researchers since quadrotor is an underactuated and a highly unstable nonlinear system. In this paper, the dynamic model of quadrotor has been derived and a so-called robust optimal backstepping control (ROBC) is designed to ...

  6. Karyotype Stability and Unbiased Fractionation in the Paleo-Allotetraploid Cucurbita Genomes.

    Science.gov (United States)

    Sun, Honghe; Wu, Shan; Zhang, Guoyu; Jiao, Chen; Guo, Shaogui; Ren, Yi; Zhang, Jie; Zhang, Haiying; Gong, Guoyi; Jia, Zhangcai; Zhang, Fan; Tian, Jiaxing; Lucas, William J; Doyle, Jeff J; Li, Haizhen; Fei, Zhangjun; Xu, Yong

    2017-10-09

    The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetraploidization event in Cucurbita. We are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the Benincaseae tribe. We estimate that the two diploid progenitors successively diverged from Benincaseae around 31 and 26 million years ago (Mya), respectively, and the allotetraploidization happened at some point between 26 Mya and 3 Mya, the estimated date when C. maxima and C. moschata diverged. The subgenomes have largely maintained the chromosome structures of their diploid progenitors. Such long-term karyotype stability after polyploidization has not been commonly observed in plant polyploids. The two subgenomes have retained similar numbers of genes, and neither subgenome is globally dominant in gene expression. Allele-specific expression analysis in the C. maxima × C. moschata interspecific F 1 hybrid and their two parents indicates the predominance of trans-regulatory effects underlying expression divergence of the parents, and detects transgressive gene expression changes in the hybrid correlated with heterosis in important agronomic traits. Our study provides insights into polyploid genome evolution and valuable resources for genetic improvement of cucurbit crops. Copyright © 2017 The Author. Published by Elsevier Inc. All rights reserved.

  7. Model predictive control of hybrid systems : stability and robustness

    NARCIS (Netherlands)

    Lazar, M.

    2006-01-01

    This thesis considers the stabilization and the robust stabilization of certain classes of hybrid systems using model predictive control. Hybrid systems represent a broad class of dynamical systems in which discrete behavior (usually described by a finite state machine) and continuous behavior

  8. Fault Tolerant Control: A Simultaneous Stabilization Result

    DEFF Research Database (Denmark)

    Stoustrup, Jakob; Blondel, V.D.

    2004-01-01

    This paper discusses the problem of designing fault tolerant compensators that stabilize a given system both in the nominal situation, as well as in the situation where one of the sensors or one of the actuators has failed. It is shown that such compensators always exist, provided that the system...... is detectable from each output and that it is stabilizable. The proof of this result is constructive, and a worked example shows how to design a fault tolerant compensator for a simple, yet challeging system. A family of second order systems is described that requires fault tolerant compensators of arbitrarily...

  9. Teleosts Genomics: Progress and Prospects in Disease Prevention and Control

    Directory of Open Access Journals (Sweden)

    Hetron Mweemba Munang’andu

    2018-04-01

    Full Text Available Genome wide studies based on conventional molecular tools and upcoming omics technologies are beginning to gain functional applications in the control and prevention of diseases in teleosts fish. Herein, we provide insights into current progress and prospects in the use genomics studies for the control and prevention of fish diseases. Metagenomics has emerged to be an important tool used to identify emerging infectious diseases for the timely design of rational disease control strategies, determining microbial compositions in different aquatic environments used for fish farming and the use of host microbiota to monitor the health status of fish. Expounding the use of antimicrobial peptides (AMPs as therapeutic agents against different pathogens as well as elucidating their role in tissue regeneration is another vital aspect of genomics studies that had taken precedent in recent years. In vaccine development, prospects made include the identification of highly immunogenic proteins for use in recombinant vaccine designs as well as identifying gene signatures that correlate with protective immunity for use as benchmarks in optimizing vaccine efficacy. Progress in quantitative trait loci (QTL mapping is beginning to yield considerable success in identifying resistant traits against some of the highly infectious diseases that have previously ravaged the aquaculture industry. Altogether, the synopsis put forth shows that genomics studies are beginning to yield positive contribution in the prevention and control of fish diseases in aquaculture.

  10. Teleosts Genomics: Progress and Prospects in Disease Prevention and Control.

    Science.gov (United States)

    Munang'andu, Hetron Mweemba; Galindo-Villegas, Jorge; David, Lior

    2018-04-04

    Genome wide studies based on conventional molecular tools and upcoming omics technologies are beginning to gain functional applications in the control and prevention of diseases in teleosts fish. Herein, we provide insights into current progress and prospects in the use genomics studies for the control and prevention of fish diseases. Metagenomics has emerged to be an important tool used to identify emerging infectious diseases for the timely design of rational disease control strategies, determining microbial compositions in different aquatic environments used for fish farming and the use of host microbiota to monitor the health status of fish. Expounding the use of antimicrobial peptides (AMPs) as therapeutic agents against different pathogens as well as elucidating their role in tissue regeneration is another vital aspect of genomics studies that had taken precedent in recent years. In vaccine development, prospects made include the identification of highly immunogenic proteins for use in recombinant vaccine designs as well as identifying gene signatures that correlate with protective immunity for use as benchmarks in optimizing vaccine efficacy. Progress in quantitative trait loci (QTL) mapping is beginning to yield considerable success in identifying resistant traits against some of the highly infectious diseases that have previously ravaged the aquaculture industry. Altogether, the synopsis put forth shows that genomics studies are beginning to yield positive contribution in the prevention and control of fish diseases in aquaculture.

  11. Stability of XIST repression in relation to genomic imprinting following global genome demethylation in a human cell line

    International Nuclear Information System (INIS)

    Araújo, E.S.S. de; Vasques, L.R.; Stabellini, R.; Krepischi, A.C.V.; Pereira, L.V.

    2014-01-01

    DNA methylation is essential in X chromosome inactivation and genomic imprinting, maintaining repression of XIST in the active X chromosome and monoallelic repression of imprinted genes. Disruption of the DNA methyltransferase genes DNMT1 and DNMT3B in the HCT116 cell line (DKO cells) leads to global DNA hypomethylation and biallelic expression of the imprinted gene IGF2 but does not lead to reactivation of XIST expression, suggesting that XIST repression is due to a more stable epigenetic mark than imprinting. To test this hypothesis, we induced acute hypomethylation in HCT116 cells by 5-aza-2′-deoxycytidine (5-aza-CdR) treatment (HCT116-5-aza-CdR) and compared that to DKO cells, evaluating DNA methylation by microarray and monitoring the expression of XIST and imprinted genes IGF2, H19, and PEG10. Whereas imprinted genes showed biallelic expression in HCT116-5-aza-CdR and DKO cells, the XIST locus was hypomethylated and weakly expressed only under acute hypomethylation conditions, indicating the importance of XIST repression in the active X to cell survival. Given that DNMT3A is the only active DNMT in DKO cells, it may be responsible for ensuring the repression of XIST in those cells. Taken together, our data suggest that XIST repression is more tightly controlled than genomic imprinting and, at least in part, is due to DNMT3A

  12. Stability of XIST repression in relation to genomic imprinting following global genome demethylation in a human cell line

    Energy Technology Data Exchange (ETDEWEB)

    Araújo, E.S.S. de [Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP (Brazil); Centro Internacional de Pesquisa, A.C. Camargo Cancer Center, São Paulo, SP (Brazil); Vasques, L.R. [Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP (Brazil); Stabellini, R.; Krepischi, A.C.V. [Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP (Brazil); Centro Internacional de Pesquisa, A.C. Camargo Cancer Center, São Paulo, SP (Brazil); Pereira, L.V. [Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP (Brazil)

    2014-10-17

    DNA methylation is essential in X chromosome inactivation and genomic imprinting, maintaining repression of XIST in the active X chromosome and monoallelic repression of imprinted genes. Disruption of the DNA methyltransferase genes DNMT1 and DNMT3B in the HCT116 cell line (DKO cells) leads to global DNA hypomethylation and biallelic expression of the imprinted gene IGF2 but does not lead to reactivation of XIST expression, suggesting that XIST repression is due to a more stable epigenetic mark than imprinting. To test this hypothesis, we induced acute hypomethylation in HCT116 cells by 5-aza-2′-deoxycytidine (5-aza-CdR) treatment (HCT116-5-aza-CdR) and compared that to DKO cells, evaluating DNA methylation by microarray and monitoring the expression of XIST and imprinted genes IGF2, H19, and PEG10. Whereas imprinted genes showed biallelic expression in HCT116-5-aza-CdR and DKO cells, the XIST locus was hypomethylated and weakly expressed only under acute hypomethylation conditions, indicating the importance of XIST repression in the active X to cell survival. Given that DNMT3A is the only active DNMT in DKO cells, it may be responsible for ensuring the repression of XIST in those cells. Taken together, our data suggest that XIST repression is more tightly controlled than genomic imprinting and, at least in part, is due to DNMT3A.

  13. Advances in power system modelling, control and stability analysis

    CERN Document Server

    Milano, Federico

    2016-01-01

    Advances in Power System Modelling, Control and Stability Analysis captures the variety of new methodologies and technologies that are changing the way modern electric power systems are modelled, simulated and operated.

  14. Stability of position control system in JIPP T-II

    International Nuclear Information System (INIS)

    Sakurai, Keiichi; Tanahashi, Shygo

    1980-01-01

    Computations and experiments on the stability of a feedback control system for maintaining a plasma column in equilibrium are described. The time response of the displacement of the plasma to the desired position is examined by solving the equation of motion of the plasma column. We show that the stability of the feedback control system is improved by using an additional term which represents the shift velocity of the plasma column. (author)

  15. Microgrid Stability Controller Based on Adaptive Robust Total SMC

    OpenAIRE

    Su, Xiaoling; Han, Minxiao; Guerrero, Josep M.; Sun, Hai

    2015-01-01

    This paper presents a microgrid stability controller (MSC) in order to provide existing distributed generation units (DGs) the additional functionality of working in islanding mode without changing their control strategies in grid-connected mode and to enhance the stability of the microgrid. Microgrid operating characteristics and mathematical models of the MSC indicate that the system is inherently nonlinear and time-variable. Therefore, this paper proposes an adaptive robust total sliding...

  16. Contributions to fuzzy polynomial techniques for stability analysis and control

    OpenAIRE

    Pitarch Pérez, José Luis

    2014-01-01

    The present thesis employs fuzzy-polynomial control techniques in order to improve the stability analysis and control of nonlinear systems. Initially, it reviews the more extended techniques in the field of Takagi-Sugeno fuzzy systems, such as the more relevant results about polynomial and fuzzy polynomial systems. The basic framework uses fuzzy polynomial models by Taylor series and sum-of-squares techniques (semidefinite programming) in order to obtain stability guarantees...

  17. Solar Dynamic Power System Stability Analysis and Control

    Science.gov (United States)

    Momoh, James A.; Wang, Yanchun

    1996-01-01

    The objective of this research is to conduct dynamic analysis, control design, and control performance test of solar power system. Solar power system consists of generation system and distribution network system. A bench mark system is used in this research, which includes a generator with excitation system and governor, an ac/dc converter, six DDCU's and forty-eight loads. A detailed model is used for modeling generator. Excitation system is represented by a third order model. DDCU is represented by a seventh order system. The load is modeled by the combination of constant power and constant impedance. Eigen-analysis and eigen-sensitivity analysis are used for system dynamic analysis. The effects of excitation system, governor, ac/dc converter control, and the type of load on system stability are discussed. In order to improve system transient stability, nonlinear ac/dc converter control is introduced. The direct linearization method is used for control design. The dynamic analysis results show that these controls affect system stability in different ways. The parameter coordination of controllers are recommended based on the dynamic analysis. It is concluded from the present studies that system stability is improved by the coordination of control parameters and the nonlinear ac/dc converter control stabilize system oscillation caused by the load change and system fault efficiently.

  18. The expanding universe of cohesin functions: a new genome stability caretaker involved in human disease and cancer.

    Science.gov (United States)

    Mannini, Linda; Menga, Stefania; Musio, Antonio

    2010-06-01

    Cohesin is responsible for sister chromatid cohesion, ensuring the correct chromosome segregation. Beyond this role, cohesin and regulatory cohesin genes seem to play a role in preserving genome stability and gene transcription regulation. DNA damage is thought to be a major culprit for many human diseases, including cancer. Our present knowledge of the molecular basis underlying genome instability is extremely limited. Mutations in cohesin genes cause human diseases such as Cornelia de Lange syndrome and Roberts syndrome/SC phocomelia, and all the cell lines derived from affected patients show genome instability. Cohesin mutations have also been identified in colorectal cancer. Here, we will discuss the human disorders caused by alterations of cohesin function, with emphasis on the emerging role of cohesin as a genome stability caretaker.

  19. Ddb1 controls genome stability and meiosis in fission yeast

    DEFF Research Database (Denmark)

    Holmberg, Christian Henrik; Fleck, Oliver; Hansen, H. A.

    2005-01-01

    The human UV-damaged DNA-binding protein Ddb1 associates with cullin 4 ubiquitin ligases implicated in nucleotide excision repair (NER). These complexes also contain the signalosome (CSN), but NER-relevant ubiquitination targets have not yet been identified. We report that fission yeast Ddb1......, Cullin 4 (Pcu4), and CSN subunits Csn1 and Csn2 are required for degradation of the ribonucleotide reductase (RNR) inhibitor protein Spd1. Ddb1-deficient cells have >20-fold increased spontaneous mutation rate. This is partly dependent on the error-prone translesion DNA polymerases. Spd1 deletion...... substantially reduced the mutation rate, suggesting that insufficient RNR activity accounts for ~50% of observed mutations. Epistasis analysis indicated that Ddb1 contributed to mutation avoidance and tolerance to DNA damage in a pathway distinct from NER. Finally, we show that Ddb1/Csn1/Cullin 4-mediated Spd1...

  20. Research on Design of MUH Attitude Stability Augmentation Control System

    Science.gov (United States)

    Fan, Shigang

    2017-09-01

    Attitude stability augmentation control system with a lower cost need to be designed so that MUH (Mini Unmanned Helicopter) can adapt to different types of geographic environment and fly steadily although the weather may be bad. Attitude feedback was calculated mainly by filtering estimation within attitude acquisition module in this system. Stability augmentation can be improved mainly by PI. This paper will depict running principle and designing process of MUH attitude stability augmentation control system and algorithm that is considered as an important part in this system.

  1. Optimal boundary control and boundary stabilization of hyperbolic systems

    CERN Document Server

    Gugat, Martin

    2015-01-01

    This brief considers recent results on optimal control and stabilization of systems governed by hyperbolic partial differential equations, specifically those in which the control action takes place at the boundary.  The wave equation is used as a typical example of a linear system, through which the author explores initial boundary value problems, concepts of exact controllability, optimal exact control, and boundary stabilization.  Nonlinear systems are also covered, with the Korteweg-de Vries and Burgers Equations serving as standard examples.  To keep the presentation as accessible as possible, the author uses the case of a system with a state that is defined on a finite space interval, so that there are only two boundary points where the system can be controlled.  Graduate and post-graduate students as well as researchers in the field will find this to be an accessible introduction to problems of optimal control and stabilization.

  2. Stability analysis of embedded nonlinear predictor neural generalized predictive controller

    Directory of Open Access Journals (Sweden)

    Hesham F. Abdel Ghaffar

    2014-03-01

    Full Text Available Nonlinear Predictor-Neural Generalized Predictive Controller (NGPC is one of the most advanced control techniques that are used with severe nonlinear processes. In this paper, a hybrid solution from NGPC and Internal Model Principle (IMP is implemented to stabilize nonlinear, non-minimum phase, variable dead time processes under high disturbance values over wide range of operation. Also, the superiority of NGPC over linear predictive controllers, like GPC, is proved for severe nonlinear processes over wide range of operation. The necessary conditions required to stabilize NGPC is derived using Lyapunov stability analysis for nonlinear processes. The NGPC stability conditions and improvement in disturbance suppression are verified by both simulation using Duffing’s nonlinear equation and real-time using continuous stirred tank reactor. Up to our knowledge, the paper offers the first hardware embedded Neural GPC which has been utilized to verify NGPC–IMP improvement in realtime.

  3. Stability of DC Voltage Droop Controllers in VSC HVDC Systems

    DEFF Research Database (Denmark)

    Thams, Florian; Suul, Jon Are; D’Arco, Salvatore

    2015-01-01

    Future multi-terminal HVDC systems are expected to utilize dc voltage droop controllers and several control implementations have been proposed in literature. This paper first classifies possible dc droop implementations in a simple framework. Then, the small-signal stability of a VSC-based conver......Future multi-terminal HVDC systems are expected to utilize dc voltage droop controllers and several control implementations have been proposed in literature. This paper first classifies possible dc droop implementations in a simple framework. Then, the small-signal stability of a VSC...

  4. Spherical gyroscopic moment stabilizer for attitude control of microsatellites

    Science.gov (United States)

    Keshtkar, Sajjad; Moreno, Jaime A.; Kojima, Hirohisa; Uchiyama, Kenji; Nohmi, Masahiro; Takaya, Keisuke

    2018-02-01

    This paper presents a new and improved concept of recently proposed two-degrees of freedom spherical stabilizer for triaxial orientation of microsatellites. The analytical analysis of the advantages of the proposed mechanism over the existing inertial attitude control devices are introduced. The extended equations of motion of the stabilizing satellite including the spherical gyroscope, for control law design and numerical simulations, are studied in detail. A new control algorithm based on continuous high-order sliding mode algorithms, for managing the torque produced by the stabilizer and therefore the attitude control of the satellite in the presence of perturbations/uncertainties, is presented. Some numerical simulations are carried out to prove the performance of the proposed mechanism and control laws.

  5. Stabilizing device for control rod tip

    International Nuclear Information System (INIS)

    Verdone, G.F.

    1982-01-01

    A control rod has a spring device on its lower end for eliminating oscillatory contact of the rod against its adjacent guide tube wall. The base of the device is connected to the lower tip of the rod. A plurality of elongated extensions are cantilevered downward from the base. Each extension has a shoulder for contacting the guide tube, and the plurality of shoulders as a group has a transverse dimension that is preset to be larger than the inner diameter of the guide tube such that an interference fit is obtained when the control rod is inserted in the tube. The elongated extensions form an open-ended, substantially hollow member through which most of the liquid coolant flows, and the spaces between adjacent extensions allow the flow to bypass the shoulders without experiencing a significant pressure drop

  6. Hummingbirds control hovering flight by stabilizing visual motion.

    Science.gov (United States)

    Goller, Benjamin; Altshuler, Douglas L

    2014-12-23

    Relatively little is known about how sensory information is used for controlling flight in birds. A powerful method is to immerse an animal in a dynamic virtual reality environment to examine behavioral responses. Here, we investigated the role of vision during free-flight hovering in hummingbirds to determine how optic flow--image movement across the retina--is used to control body position. We filmed hummingbirds hovering in front of a projection screen with the prediction that projecting moving patterns would disrupt hovering stability but stationary patterns would allow the hummingbird to stabilize position. When hovering in the presence of moving gratings and spirals, hummingbirds lost positional stability and responded to the specific orientation of the moving visual stimulus. There was no loss of stability with stationary versions of the same stimulus patterns. When exposed to a single stimulus many times or to a weakened stimulus that combined a moving spiral with a stationary checkerboard, the response to looming motion declined. However, even minimal visual motion was sufficient to cause a loss of positional stability despite prominent stationary features. Collectively, these experiments demonstrate that hummingbirds control hovering position by stabilizing motions in their visual field. The high sensitivity and persistence of this disruptive response is surprising, given that the hummingbird brain is highly specialized for sensory processing and spatial mapping, providing other potential mechanisms for controlling position.

  7. DNA replication factor C1 mediates genomic stability and transcriptional gene silencing in Arabidopsis

    KAUST Repository

    Liu, Qian; Wang, Junguo; Miki, Daisuke; Xia, Ran; Yu, Wenxiang; He, Junna; Zheng, Zhimin; Zhu, Jian-Kang; Gonga, Zhizhong

    2010-01-01

    Genetic screening identified a suppressor of ros1-1, a mutant of REPRESSOR OF SILENCING1 (ROS1; encoding a DNA demethylation protein). The suppressor is a mutation in the gene encoding the largest subunit of replication factor C (RFC1). This mutation of RFC1 reactivates the unlinked 35S-NPTII transgene, which is silenced in ros1 and also increases expression of the pericentromeric Athila retrotransposons named transcriptional silent information in a DNA methylationindependent manner. rfc1 is more sensitive than the wild type to the DNA-damaging agent methylmethane sulphonate and to the DNA inter- and intra- cross-linking agent cisplatin. The rfc1 mutant constitutively expresses the G2/M-specific cyclin CycB1;1 and other DNA repair-related genes. Treatment with DNA-damaging agents mimics the rfc1 mutation in releasing the silenced 35S-NPTII, suggesting that spontaneously induced genomic instability caused by the rfc1 mutation might partially contribute to the released transcriptional gene silencing (TGS). The frequency of somatic homologous recombination is significantly increased in the rfc1 mutant. Interestingly, ros1 mutants show increased telomere length, but rfc1 mutants show decreased telomere length and reduced expression of telomerase. Our results suggest that RFC1 helps mediate genomic stability and TGS in Arabidopsis thaliana. © 2010 American Society of Plant Biologists.

  8. DNA replication factor C1 mediates genomic stability and transcriptional gene silencing in Arabidopsis

    KAUST Repository

    Liu, Qian

    2010-07-01

    Genetic screening identified a suppressor of ros1-1, a mutant of REPRESSOR OF SILENCING1 (ROS1; encoding a DNA demethylation protein). The suppressor is a mutation in the gene encoding the largest subunit of replication factor C (RFC1). This mutation of RFC1 reactivates the unlinked 35S-NPTII transgene, which is silenced in ros1 and also increases expression of the pericentromeric Athila retrotransposons named transcriptional silent information in a DNA methylationindependent manner. rfc1 is more sensitive than the wild type to the DNA-damaging agent methylmethane sulphonate and to the DNA inter- and intra- cross-linking agent cisplatin. The rfc1 mutant constitutively expresses the G2/M-specific cyclin CycB1;1 and other DNA repair-related genes. Treatment with DNA-damaging agents mimics the rfc1 mutation in releasing the silenced 35S-NPTII, suggesting that spontaneously induced genomic instability caused by the rfc1 mutation might partially contribute to the released transcriptional gene silencing (TGS). The frequency of somatic homologous recombination is significantly increased in the rfc1 mutant. Interestingly, ros1 mutants show increased telomere length, but rfc1 mutants show decreased telomere length and reduced expression of telomerase. Our results suggest that RFC1 helps mediate genomic stability and TGS in Arabidopsis thaliana. © 2010 American Society of Plant Biologists.

  9. Response of normal stem cells to ionizing radiation: A balance between homeostasis and genomic stability

    International Nuclear Information System (INIS)

    Harfouche, G.; Martin, M.T.

    2010-01-01

    Stem cells have been described in most adult tissues, where they play a key role in maintaining tissue homeostasis. As they self-renew throughout life, accumulating genetic anomalies can compromise their genomic integrity and potentially give rise to cancer. Stem cells (SCs) may thus be a major target of radiation carcinogenesis. In addition, unrepaired genotoxic damage may cause cell death and stem cell pool depletion, impairing lineage functionality and accelerating aging. Developments in SC biology enabled the characterization of the responses of stem cells to genotoxic stress and their role in tissue damage. We here examine how these cells react to ionizing radiation (IR), and more specifically their radiosensitivity, stress signaling and DNA repair. We first review embryonic SCs, as a paradigm of primitive pluri-potent cells, then three adult tissues, bone marrow, skin and intestine, capable of long-term regeneration and at high risk for acute radiation syndromes and long-term carcinogenesis. We discuss IR disruption of the fine balance between maintenance of tissue homeostasis and genomic stability. We show that stem cell radiosensitivity does not follow a unique model, but differs notably according to the turnover rates of the tissues. (authors)

  10. Stabilization of rotational motion with application to spacecraft attitude control

    DEFF Research Database (Denmark)

    Wisniewski, Rafal

    2000-01-01

    for global stabilization of a rotary motion. Along with a model of the system formulated in the Hamilton's canonical from the algorithm uses information about a required potential energy and a dissipation term. The control action is the sum of the gradient of the potential energy and the dissipation force......The objective of this paper is to develop a control scheme for stabilization of a hamiltonian system. The method generalizes the results available in the literature on motion control in the Euclidean space to an arbitrary differrential manifol equipped with a metric. This modification is essencial...... on a Riemannian manifold. The Lyapnov stability theory is adapted and reformulated to fit to the new framework of Riemannian manifolds. Toillustrate the results a spacecraft attitude control problem is considered. Firstly, a global canonical representation for the spacecraft motion is found, then three spacecraft...

  11. Stabilization of rotational motion with application to spacecraft attitude control

    DEFF Research Database (Denmark)

    Wisniewski, Rafal

    2001-01-01

    for global stabilization of a rotary motion. Along with a model of the system formulated in the Hamilton's canonical from the algorithm uses information about a required potential energy and a dissipation term. The control action is the sum of the gradient of the potential energy and the dissipation force......The objective of this paper is to develop a control scheme for stabilization of a hamiltonian system. The method generalizes the results available in the literature on motion control in the Euclidean space to an arbitrary differrential manifol equipped with a metric. This modification is essencial...... on a Riemannian manifold. The Lyapnov stability theory is adapted and reformulated to fit to the new framework of Riemannian manifolds. Toillustrate the results a spacecraft attitude control problem is considered. Firstly, a global canonical representation for the spacecraft motion is found, then three spacecraft...

  12. A Decision Support Tool for Transient Stability Preventive Control

    DEFF Research Database (Denmark)

    Pertl, Michael; Weckesser, Johannes Tilman Gabriel; Rezkalla, Michel M.N.

    2017-01-01

    The paper presents a decision support tool for transient stability preventive control contributing to increased situation awareness of control room operators by providing additional information about the state of the power system in terms of transient stability. A time-domain approach is used...... a predefined minimum critical clearing time for faults at all buses is proposed, while costs are minimized. The results of the assessment are presented to the control room operator, who decides to accept the suggested dispatch or to repeat the assessment considering additional user-specific constraints...

  13. Power system small signal stability analysis and control

    CERN Document Server

    Mondal, Debasish; Sengupta, Aparajita

    2014-01-01

    Power System Small Signal Stability Analysis and Control presents a detailed analysis of the problem of severe outages due to the sustained growth of small signal oscillations in modern interconnected power systems. The ever-expanding nature of power systems and the rapid upgrade to smart grid technologies call for the implementation of robust and optimal controls. Power systems that are forced to operate close to their stability limit have resulted in the use of control devices by utility companies to improve the performance of the transmission system against commonly occurring power system

  14. An active interferometer-stabilization scheme with linear phase control

    DEFF Research Database (Denmark)

    Andresen, Esben Ravn; Krishnamachari, v v; Potma, E O

    2006-01-01

    We report a simple and robust computer-based active interferometer stabilization scheme which does not require modulation of the interfering beams and relies on an error signal which is linearly related to the optical path difference. In this setup, a non-collinearly propagating reference laser...... beam stabilizes the interference output of the laser light propagating collinearly through the interferometer. This stabilization scheme enables adjustable phase control with 20 ms switching times in the range from 0.02π radians to 6π radians at 632.8 nm....

  15. Biomass Combustion Control and Stabilization Using Low-Cost Sensors

    Directory of Open Access Journals (Sweden)

    Ján Piteľ

    2013-01-01

    Full Text Available The paper describes methods for biomass combustion process control and burning stabilization based on low-cost sensing of carbon monoxide emissions and oxygen concentration in the flue gas. The designed control system was tested on medium-scale biomass-fired boilers and some results are evaluated and presented in the paper.

  16. Stability Analysis for Car Following Model Based on Control Theory

    International Nuclear Information System (INIS)

    Meng Xiang-Pei; Li Zhi-Peng; Ge Hong-Xia

    2014-01-01

    Stability analysis is one of the key issues in car-following theory. The stability analysis with Lyapunov function for the two velocity difference car-following model (for short, TVDM) is conducted and the control method to suppress traffic congestion is introduced. Numerical simulations are given and results are consistent with the theoretical analysis. (electromagnetism, optics, acoustics, heat transfer, classical mechanics, and fluid dynamics)

  17. Control Design of a Nonlinear Controller to Stabilize the Nonlinear ...

    African Journals Online (AJOL)

    inyangs

    is used to formularize the delay differential equation in equation. (13), which accounts for the delayed terms in the system. By applying the Leibniz integration rule, the derivative. •. V shows that the system is asymptotically stable with a negative definite solution in equation (14). The conditions for the stability are: •. The delay ...

  18. DNA damage checkpoint kinase ATM regulates germination and maintains genome stability in seeds.

    Science.gov (United States)

    Waterworth, Wanda M; Footitt, Steven; Bray, Clifford M; Finch-Savage, William E; West, Christopher E

    2016-08-23

    Genome integrity is crucial for cellular survival and the faithful transmission of genetic information. The eukaryotic cellular response to DNA damage is orchestrated by the DNA damage checkpoint kinases ATAXIA TELANGIECTASIA MUTATED (ATM) and ATM AND RAD3-RELATED (ATR). Here we identify important physiological roles for these sensor kinases in control of seed germination. We demonstrate that double-strand breaks (DSBs) are rate-limiting for germination. We identify that desiccation tolerant seeds exhibit a striking transcriptional DSB damage response during germination, indicative of high levels of genotoxic stress, which is induced following maturation drying and quiescence. Mutant atr and atm seeds are highly resistant to aging, establishing ATM and ATR as determinants of seed viability. In response to aging, ATM delays germination, whereas atm mutant seeds germinate with extensive chromosomal abnormalities. This identifies ATM as a major factor that controls germination in aged seeds, integrating progression through germination with surveillance of genome integrity. Mechanistically, ATM functions through control of DNA replication in imbibing seeds. ATM signaling is mediated by transcriptional control of the cell cycle inhibitor SIAMESE-RELATED 5, an essential factor required for the aging-induced delay to germination. In the soil seed bank, seeds exhibit increased transcript levels of ATM and ATR, with changes in dormancy and germination potential modulated by environmental signals, including temperature and soil moisture. Collectively, our findings reveal physiological functions for these sensor kinases in linking genome integrity to germination, thereby influencing seed quality, crucial for plant survival in the natural environment and sustainable crop production.

  19. Power system stability enhancement using facts controllers: a review

    International Nuclear Information System (INIS)

    Abido, M. A

    2009-01-01

    In recent years, power demand has increased substantially while the expansion of power generation and transmission has been severely limited due to limited resources and environmental restrictions. As a consequence, some transmission lines are heavily loaded and the system stability becomes a power transfer-limiting factor. Flexible AC transmission systems (FACTS) controllers have been mainly used for solving various power system steady state control problems. However, recent studies reveal that FACTS controllers could be employed to enhance power system stability in addition to their main function of power flow control. The literature shows an increasing interest in this subject for the last two decades, where the enhancement of system stability using FACTS controllers has been extensively investigated. This paper presents a comprehensive review on the research and developments in the power system stability enhancement using FACTS damping controllers. Several technical issues related to FACTS installations have been highlighted and performance comparison of different FACTS controllers has been discussed. In addition, some of the utility experience, real-world installations, and semiconductor technology development have been reviewed and summarized. Applications of FACTS to other power system studies have also been discussed. About two hundred twenty seven research publications have been classified and appended for a quick reference. (author)

  20. Global insights into acetic acid resistance mechanisms and genetic stability of Acetobacter pasteurianus strains by comparative genomics

    Science.gov (United States)

    Wang, Bin; Shao, Yanchun; Chen, Tao; Chen, Wanping; Chen, Fusheng

    2015-12-01

    Acetobacter pasteurianus (Ap) CICC 20001 and CGMCC 1.41 are two acetic acid bacteria strains that, because of their strong abilities to produce and tolerate high concentrations of acetic acid, have been widely used to brew vinegar in China. To globally understand the fermentation characteristics, acid-tolerant mechanisms and genetic stabilities, their genomes were sequenced. Genomic comparisons with 9 other sequenced Ap strains revealed that their chromosomes were evolutionarily conserved, whereas the plasmids were unique compared with other Ap strains. Analysis of the acid-tolerant metabolic pathway at the genomic level indicated that the metabolism of some amino acids and the known mechanisms of acetic acid tolerance, might collaboratively contribute to acetic acid resistance in Ap strains. The balance of instability factors and stability factors in the genomes of Ap CICC 20001 and CGMCC 1.41 strains might be the basis for their genetic stability, consistent with their stable industrial performances. These observations provide important insights into the acid resistance mechanism and the genetic stability of Ap strains and lay a foundation for future genetic manipulation and engineering of these two strains.

  1. Eco-geomorphic controls on slope stability

    Science.gov (United States)

    Hales, T.; Ford, C.; Hwang, T.; Vose, J.; Band, L.

    2009-04-01

    Vegetation controls soil-mantled landscape evolution primarily through growth of roots into soil and rock. Root-soil interactions affect the spatial distribution and rate of shallow landsliding and other hillslope processes. Yet the distribution and tensile strength of roots depends on a number of geomorphically-influenced parameters, including soil moisture. Our field-based study investigated the effects of topography on root distributions, tensile strengths, and cohesion. Systematic differences in plant species distribution and soil properties are found in the hollow-nose topography of soil-mantled landscapes; with hollows containing thick colluvial soils and mesic tree species and noses containing thinner, more differentiated soils and more xeric species. We investigated whether these topographic variations in geomorphic and ecologic properties affected the spatial distribution of root cohesion by measuring the distribution and tensile strength of roots from soil pits dug downslope of fifteen individual trees in the Coweeta Hydrologic Laboratory, North Carolina. Our soil pits were located to capture variance in plant species (10 species total), topographic positions (nose, hollow), and sizes (a range of DBH between 5 cm and 60 cm). Root tensile strengths showed little variance with different species, but showed strong differences as a function of topography, with nose roots stronger than hollow roots. Similarly, within species, root cellulose content was systematically greater in trees on nose positions compared to those in hollows. For all species, roots were concentrated close to the soil surface (at least 70% of biomass occurred within 50 cm of the surface) and variations in this pattern were primarily a function of topographic position. Hollow roots were more evenly distributed in the soil column than those on noses, yet trees located on noses had higher mean root cohesion than those in hollows because of a higher root tensile force. These data provide an

  2. Super DNAging-New insights into DNA integrity, genome stability and telomeres in the oldest old.

    Science.gov (United States)

    Franzke, Bernhard; Neubauer, Oliver; Wagner, Karl-Heinz

    2015-01-01

    Reductions in DNA integrity, genome stability, and telomere length are strongly associated with the aging process, age-related diseases as well as the age-related loss of muscle mass. However, in people reaching an age far beyond their statistical life expectancy the prevalence of diseases, such as cancer, cardiovascular disease, diabetes or dementia, is much lower compared to "averagely" aged humans. These inverse observations in nonagenarians (90-99 years), centenarians (100-109 years) and super-centenarians (110 years and older) require a closer look into dynamics underlying DNA damage within the oldest old of our society. Available data indicate improved DNA repair and antioxidant defense mechanisms in "super old" humans, which are comparable with much younger cohorts. Partly as a result of these enhanced endogenous repair and protective mechanisms, the oldest old humans appear to cope better with risk factors for DNA damage over their lifetime compared to subjects whose lifespan coincides with the statistical life expectancy. This model is supported by study results demonstrating superior chromosomal stability, telomere dynamics and DNA integrity in "successful agers". There is also compelling evidence suggesting that life-style related factors including regular physical activity, a well-balanced diet and minimized psycho-social stress can reduce DNA damage and improve chromosomal stability. The most conclusive picture that emerges from reviewing the literature is that reaching "super old" age appears to be primarily determined by hereditary/genetic factors, while a healthy lifestyle additionally contributes to achieving the individual maximum lifespan in humans. More research is required in this rapidly growing population of super old people. In particular, there is need for more comprehensive investigations including short- and long-term lifestyle interventions as well as investigations focusing on the mechanisms causing DNA damage, mutations, and telomere

  3. Method for rudder roll stabilization control by maintaining ship speed

    Directory of Open Access Journals (Sweden)

    LIU Zhiquan

    2017-01-01

    Full Text Available A ship navigating on the surface of the water may experience greater resistance, adversely affect-ing its speed and leading to energy loss. The added resistance of surface ships in both still water and waves are investigated, and the computation method of total speed loss is presented. An autopilot system is intro-duced to constrain the speed loss, and course keeping and rudder roll stabilization sliding mode control laws are proposed according to a compact control strategy. The two working conditions of "heading" and "heading plus anti-roll" are discussed, including roll stabilization, heading error, speed maintenance and rudder abrasion. The results show that the speed can be effectively maintained using this method, and from a commercial point of view, the fin-rudder roll stabilization control is not recommended for vessels equipped with both fins and rudders.

  4. Adaptive Dynamic Programming for Control Algorithms and Stability

    CERN Document Server

    Zhang, Huaguang; Luo, Yanhong; Wang, Ding

    2013-01-01

    There are many methods of stable controller design for nonlinear systems. In seeking to go beyond the minimum requirement of stability, Adaptive Dynamic Programming for Control approaches the challenging topic of optimal control for nonlinear systems using the tools of  adaptive dynamic programming (ADP). The range of systems treated is extensive; affine, switched, singularly perturbed and time-delay nonlinear systems are discussed as are the uses of neural networks and techniques of value and policy iteration. The text features three main aspects of ADP in which the methods proposed for stabilization and for tracking and games benefit from the incorporation of optimal control methods: • infinite-horizon control for which the difficulty of solving partial differential Hamilton–Jacobi–Bellman equations directly is overcome, and  proof provided that the iterative value function updating sequence converges to the infimum of all the value functions obtained by admissible control law sequences; • finite-...

  5. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2.

    Science.gov (United States)

    Mendez-Bermudez, Aaron; Lototska, Liudmyla; Bauwens, Serge; Giraud-Panis, Marie-Josèphe; Croce, Olivier; Jamet, Karine; Irizar, Agurtzane; Mowinckel, Macarena; Koundrioukoff, Stephane; Nottet, Nicolas; Almouzni, Genevieve; Teulade-Fichou, Mare-Paule; Schertzer, Michael; Perderiset, Mylène; Londoño-Vallejo, Arturo; Debatisse, Michelle; Gilson, Eric; Ye, Jing

    2018-05-03

    Hard-to-replicate regions of chromosomes (e.g., pericentromeres, centromeres, and telomeres) impede replication fork progression, eventually leading, in the event of replication stress, to chromosome fragility, aging, and cancer. Our knowledge of the mechanisms controlling the stability of these regions is essentially limited to telomeres, where fragility is counteracted by the shelterin proteins. Here we show that the shelterin subunit TRF2 ensures progression of the replication fork through pericentromeric heterochromatin, but not centromeric chromatin. In a process involving its N-terminal basic domain, TRF2 binds to pericentromeric Satellite III sequences during S phase, allowing the recruitment of the G-quadruplex-resolving helicase RTEL1 to facilitate fork progression. We also show that TRF2 is required for the stability of other heterochromatic regions localized throughout the genome, paving the way for future research on heterochromatic replication and its relationship with aging and cancer. Copyright © 2018 Elsevier Inc. All rights reserved.

  6. Optimal Sliding Mode Controllers for Attitude Stabilization of Flexible Spacecraft

    Directory of Open Access Journals (Sweden)

    Chutiphon Pukdeboon

    2011-01-01

    Full Text Available The robust optimal attitude control problem for a flexible spacecraft is considered. Two optimal sliding mode control laws that ensure the exponential convergence of the attitude control system are developed. Integral sliding mode control (ISMC is applied to combine the first-order sliding mode with optimal control and is used to control quaternion-based spacecraft attitude manoeuvres with external disturbances and an uncertainty inertia matrix. For the optimal control part the state-dependent Riccati equation (SDRE and optimal Lyapunov techniques are employed to solve the infinite-time nonlinear optimal control problem. The second method of Lyapunov is used to guarantee the stability of the attitude control system under the action of the proposed control laws. An example of multiaxial attitude manoeuvres is presented and simulation results are included to verify the usefulness of the developed controllers.

  7. A study of offshore wind HVDC system stability and control

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Hanchao; Sun, Jian [Rensselaer Polytechnic Institute, Troy, NY (United States). Dept. of Electrical, Computer and Systems Engineering

    2011-07-01

    This work is concerned with the stability and control of ac power collection buses in offshore wind farms with high-voltage dc (HVDC) transmission connection to onshore power grid. The focus of the work is high-frequency interactions among the wind turbines, the ac collection bus and the filters, as well as the HVDC rectifier. Both voltage-source converter and line-commutated converter based HVDC systems are considered. To study high-frequency stability, particularly harmonic resonance in the ac bus, small-signal impedance models are developed for the wind inverters and the HVDC rectifier by using harmonic linearization techniques. An impedance-based stability criterion is applied to assess system stability in both positive- and negative-sequence domain. Small-signal stability conditions and requirements are developed from analytical impedance models. Detailed system-level simulation is used to validated the small-signal analysis. The goal of the study is to develop system design and control techniques that minimize the cost of the offshore infrastructure while guaranteeing system stability and power quality. (orig.)

  8. Stabilization of third-order bilinear systems using constant controls

    Directory of Open Access Journals (Sweden)

    A. E. Golubev

    2014-01-01

    Full Text Available This paper deals with the zero equilibrium stabilization for dynamical systems that have control input singularities. A dynamical system with scalar control input is called nonregular if the coefficient of input becomes null on a subset of the phase space that contains the origin. One of the classes of nonregular dynamical systems is represented by bilinear systems. In case of second-order bilinear systems the necessary and sufficient conditions for the zero equilibrium stabilizability are known in the literature. However, in general case the stabilization problem in the presence of control input singularities has not been solved yet.In this note we solve the problem of the zero equilibrium stabilization for the third-order bilinear dynamical systems given in a canonical form. The solution is found in the class of constant controls. The necessary and sufficient conditions are obtained for the zero equilibrium stabilizability of the bilinear systems in question.The dependence of the zero equilibrium stabilizability on system parameter values is analyzed. The general criteria of stabilizability by means of constant controls are given for the bilinear systems in question. In case when all the system parameters have nonzero values the necessary and sufficient stabilizability conditions are proved. The case when some of the parameters are equal to zero is also considered.Further research can be focused on extending the obtained results to a higher-order case of bilinear and affine dynamical systems. The solution of the considered stabilization problem should also be found not only within constant controls but also in a class of state feedbacks, particularly, in the case when stabilizing constant control does not exist.One of the potential application areas for the obtained theoretical results is automatic control of technical plants like unmanned aerial vehicles and mobile robots.

  9. Strong stabilization servo controller with optimization of performance criteria.

    Science.gov (United States)

    Sarjaš, Andrej; Svečko, Rajko; Chowdhury, Amor

    2011-07-01

    Synthesis of a simple robust controller with a pole placement technique and a H(∞) metrics is the method used for control of a servo mechanism with BLDC and BDC electric motors. The method includes solving a polynomial equation on the basis of the chosen characteristic polynomial using the Manabe standard polynomial form and parametric solutions. Parametric solutions are introduced directly into the structure of the servo controller. On the basis of the chosen parametric solutions the robustness of a closed-loop system is assessed through uncertainty models and assessment of the norm ‖•‖(∞). The design procedure and the optimization are performed with a genetic algorithm differential evolution - DE. The DE optimization method determines a suboptimal solution throughout the optimization on the basis of a spectrally square polynomial and Šiljak's absolute stability test. The stability of the designed controller during the optimization is being checked with Lipatov's stability condition. Both utilized approaches: Šiljak's test and Lipatov's condition, check the robustness and stability characteristics on the basis of the polynomial's coefficients, and are very convenient for automated design of closed-loop control and for application in optimization algorithms such as DE. Copyright © 2011 ISA. Published by Elsevier Ltd. All rights reserved.

  10. Robust stability bounds for multi-delay networked control systems

    Science.gov (United States)

    Seitz, Timothy; Yedavalli, Rama K.; Behbahani, Alireza

    2018-04-01

    In this paper, the robust stability of a perturbed linear continuous-time system is examined when controlled using a sampled-data networked control system (NCS) framework. Three new robust stability bounds on the time-invariant perturbations to the original continuous-time plant matrix are presented guaranteeing stability for the corresponding discrete closed-loop augmented delay-free system (ADFS) with multiple time-varying sensor and actuator delays. The bounds are differentiated from previous work by accounting for the sampled-data nature of the NCS and for separate communication delays for each sensor and actuator, not a single delay. Therefore, this paper expands the knowledge base in multiple inputs multiple outputs (MIMO) sampled-data time delay systems. Bounds are presented for unstructured, semi-structured, and structured perturbations.

  11. Stability and servo-control of the crystal pulling process

    International Nuclear Information System (INIS)

    Johansen, T.H.

    1990-11-01

    The paper analyzes why the crystal pulling process needs servo-control, and how it can be implemented. Special emphasis is put on the fundamental question of inherent stability, and how to interpret the signal from a balance when the weighing method is used for cystal diameter detection. 15 refs., 13 figs

  12. Stability analysis of direct current control in current source rectifier

    DEFF Research Database (Denmark)

    Lu, Dapeng; Wang, Xiongfei; Blaabjerg, Frede

    2017-01-01

    Current source rectifier with high switching frequency has a great potential for improving the power efficiency and power density in ac-dc power conversion. This paper analyzes the stability of direct current control based on the time delay effect. Small signal model including dynamic behaviors...

  13. Robust stabilization of nonlinear systems by quantized and ternary control

    NARCIS (Netherlands)

    Persis, Claudio De

    2009-01-01

    Results on the problem of stabilizing a nonlinear continuous-time minimum-phase system by a finite number of control or measurement values are presented. The basic tool is a discontinuous version of the so-called semi-global backstepping lemma. We derive robust practical stabilizability results by

  14. Synthetic Jets Flow Control on a vertical stabilizer

    Science.gov (United States)

    Rathay, Nicholas; Boucher, Matthew; Amitay, Michael

    2011-11-01

    The vertical stabilizer on most commercial transport aircraft is much larger than required for stability and control. The tail is significantly oversized in order to maintain controllability in the event of asymmetric engine failure and meet flying qualities requirements related to dynamic motion. Using aerodynamic flow control techniques, it may be possible to reduce the size of the tail while maintaining similar control authority during inclement flight conditions. Reducing the size of the tail decreases the weight and the drag of the airplane, which results in considerable savings in fuel costs. In this work, it is shown that synthetic jet (zero-net-mass-flux) actuators are capable of reattaching the separated flow on the rudder and augmenting the performance of the stabilizer. Experiments were conducted in an open-return wind tunnel on a 1/25th scale model of a vertical stabilizer and a partial fuselage section. The surface pressure, aerodynamic loads and data acquired with a Stereo PIV system were used to investigate the effectiveness of this technology as well as provide a more detailed analysis of the flowfield and showed that the synthetic jets are capable of augmenting the side-force by up to 20%.

  15. Microgrid Stability Controller Based on Adaptive Robust Total SMC

    DEFF Research Database (Denmark)

    Su, Xiaoling; Han, Minxiao; Guerrero, Josep M.

    2015-01-01

    This paper presents a microgrid stability controller (MSC) in order to provide existing DGs the additional functionality of working in islanding mode without changing their control strategies in grid-connected mode and to enhance the stability of the microgrid. Microgrid operating characteristics....... The MSC provides fast dynamic response and robustness to the microgrid. When the system is operating in grid-connected mode, it is able to improve the controllability of the exchanged power between the microgrid and the utility grid, while smoothing DG’s output power. When the microgrid is operating...... and mathematical models of the MSC indicate that the system is inherently nonlinear and time-variable. Therefore, this paper proposes an adaptive robust total sliding-mode control (ARTSMC) system for the MSC. It is proved that the ARTSMC system is insensitive to parametric uncertainties and external disturbances...

  16. Doping control container for urine stabilization: a pilot study.

    Science.gov (United States)

    Tsivou, Maria; Giannadaki, Evangelia; Hooghe, Fiona; Roels, Kris; Van Gansbeke, Wim; Garribba, Flaminia; Lyris, Emmanouil; Deventer, Koen; Mazzarino, Monica; Donati, Francesco; Georgakopoulos, Dimitrios G; Van Eenoo, Peter; Georgakopoulos, Costas G; de la Torre, Xavier; Botrè, Francesco

    2017-05-01

    Urine collection containers used in the doping control collection procedure do not provide a protective environment for urine, against degradation by microorganisms and proteolytic enzymes. An in-house chemical stabilization mixture was developed to tackle urine degradation problems encountered in human sport samples, in cases of microbial contamination or proteolytic activity. The mixture consists of antimicrobial substances and protease inhibitors for the simultaneous inactivation of a wide range of proteolytic enzymes. It has already been tested in lab-scale, as part of World Anti-Doping Agency's (WADA) funded research project, in terms of efficiency against microbial and proteolytic activity. The present work, funded also by WADA, is a follow-up study on the improvement of chemical stabilization mixture composition, application mode and limitation of interferences, using pilot urine collection containers, spray-coated in their internal surface with the chemical stabilization mixture. Urine in plastic stabilized collection containers have been gone through various incubation cycles to test for stabilization efficiency and analytical matrix interferences by three WADA accredited Laboratories (Athens, Ghent, and Rome). The spray-coated chemical stabilization mixture was tested against microorganism elimination and steroid glucuronide degradation, as well as enzymatic breakdown of proteins, such as intact hCG, recombinant erythropoietin and small peptides (GHRPs, ipamorelin), induced by proteolytic enzymes. Potential analytical interferences, observed in the presence of spray-coated chemical stabilization mixture, were recorded using routine screening procedures. The results of the current study support the application of the spray-coated plastic urine container, in the doping control collection procedure. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  17. Stability Control of Vehicle Emergency Braking with Tire Blowout

    OpenAIRE

    Chen, Qingzhang; Liu, Youhua; Li, Xuezhi

    2014-01-01

    For the stability control and slowing down the vehicle to a safe speed after tire failure, an emergency automatic braking system with independent intellectual property is developed. After the system has received a signal of tire blowout, the automatic braking mode of the vehicle is determined according to the position of the failure tire and the motion state of vehicle, and a control strategy for resisting tire blowout additional yaw torque and deceleration is designed to slow down vehicle to...

  18. Fuzzy logic controller for stabilization of biped robot gait

    Directory of Open Access Journals (Sweden)

    Ryadchikov I.V.

    2018-01-01

    Full Text Available The article centers round the problem of stabilization of biped robot gait through smoothing out the jumps of first and second order derivatives of a biped robot control vector using the fuzzy logic approach. The structure of a composite Takagi-Sugeno fuzzy logic controller developed by the authors is presented. The simulation study of a robot gait with climbing an obstacle is carried out and the results provided in the article showed that the developed controller performed significantly better than the analytical formula model in terms of smoothing out the derivatives of the control vector.

  19. The electric power engineering handbook power system stability and control

    CERN Document Server

    Grisby, Leonard L

    2012-01-01

    With contributions from worldwide leaders in the field, Power System Stability and Control, Third Edition (part of the five-volume set, The Electric Power Engineering Handbook) updates coverage of recent developments and rapid technological growth in essential aspects of power systems. Edited by L.L. Grigsby, a respected and accomplished authority in power engineering, and section editors Miroslav Begovic, Prabha Kundur, and Bruce Wollenberg, this reference presents substantially new and revised content. Topics covered include: * Power System Protection * Power System Dynamics and Stability *

  20. Stabilization and Synchronization of Memristive Chaotic Circuits by Impulsive Control

    Directory of Open Access Journals (Sweden)

    Limin Zou

    2017-01-01

    Full Text Available The purpose of this note is to study impulsive control and synchronization of memristor based chaotic circuits shown by Muthuswamy. We first establish a less conservative sufficient condition for the stability of memristor based chaotic circuits. After that, we discuss the effect of errors on stability. Meanwhile, we also discuss impulsive synchronization of two memristor based chaotic systems. Our results are more general and more applicable than the ones shown by Yang, Li, and Huang. Finally, several numerical examples are given to show the effectiveness of our methods.

  1. Robust stability in predictive control with soft constraints

    DEFF Research Database (Denmark)

    Thomsen, Sven Creutz; Niemann, Hans Henrik; Poulsen, Niels Kjølstad

    2010-01-01

    In this paper we take advantage of the primary and dual Youla parameterizations for setting up a soft constrained model predictive control (MPC) scheme for which stability is guaranteed in face of norm-bounded uncertainties. Under special conditions guarantees are also given for hard input...... constraints. In more detail, we parameterize the MPC predictions in terms of the primary Youla parameter and use this parameter as the online optimization variable. The uncertainty is parameterized in terms of the dual Youla parameter. Stability can then be guaranteed through small gain arguments on the loop...

  2. Robust stability in constrained predictive control through the Youla parameterisations

    DEFF Research Database (Denmark)

    Thomsen, Sven Creutz; Niemann, Hans Henrik; Poulsen, Niels Kjølstad

    2011-01-01

    In this article we take advantage of the primary and dual Youla parameterisations to set up a soft constrained model predictive control (MPC) scheme. In this framework it is possible to guarantee stability in face of norm-bounded uncertainties. Under special conditions guarantees are also given...... for hard input constraints. In more detail, we parameterise the MPC predictions in terms of the primary Youla parameter and use this parameter as the on-line optimisation variable. The uncertainty is parameterised in terms of the dual Youla parameter. Stability can then be guaranteed through small gain...

  3. p53 Maintains Genomic Stability by Preventing Interference between Transcription and Replication

    Directory of Open Access Journals (Sweden)

    Constance Qiao Xin Yeo

    2016-04-01

    Full Text Available p53 tumor suppressor maintains genomic stability, typically acting through cell-cycle arrest, senescence, and apoptosis. We discovered a function of p53 in preventing conflicts between transcription and replication, independent of its canonical roles. p53 deficiency sensitizes cells to Topoisomerase (Topo II inhibitors, resulting in DNA damage arising spontaneously during replication. Topoisomerase IIα (TOP2A-DNA complexes preferentially accumulate in isogenic p53 mutant or knockout cells, reflecting an increased recruitment of TOP2A to regulate DNA topology. We propose that p53 acts to prevent DNA topological stress originating from transcription during the S phase and, therefore, promotes normal replication fork progression. Consequently, replication fork progression is impaired in the absence of p53, which is reversed by transcription inhibition. Pharmacologic inhibition of transcription also attenuates DNA damage and decreases Topo-II-DNA complexes, restoring cell viability in p53-deficient cells. Together, our results demonstrate a function of p53 that may underlie its role in tumor suppression.

  4. Stability and Control of Human Trunk Movement During Walking.

    Science.gov (United States)

    Wu, Q.; Sepehri, N.; Thornton-Trump, A. B.; Alexander, M.

    1998-01-01

    A mathematical model has been developed to study the control mechanisms of human trunk movement during walking. The trunk is modeled as a base-excited inverted pendulum with two-degrees of rotational freedom. The base point, corresponding to the bony landmark of the sacrum, can move in three-dimensional space in a general way. Since the stability of upright posture is essential for human walking, a controller has been designed such that the stability of the pendulum about the upright position is guaranteed. The control laws are developed based on Lyapunov's stability theory and include feedforward and linear feedback components. It is found that the feedforward component plays a critical role in keeping postural stability, and the linear feedback component, (resulting from viscoelastic function of the musculoskeletal system) can effectively duplicate the pattern of trunk movement. The mathematical model is validated by comparing the simulation results with those based on gait measurements performed in the Biomechanics Laboratory at the University of Manitoba.

  5. Streamlining genomes: toward the generation of simplified and stabilized microbial systems

    NARCIS (Netherlands)

    Leprince, A.; Passel, van M.W.J.; Martins Dos Santos, V.A.P.

    2012-01-01

    At the junction between systems and synthetic biology, genome streamlining provides a solid foundation both for increased understanding of cellular circuitry, and for the tailoring of microbial chassis towards innovative biotechnological applications. Iterative genomic deletions (targeted and

  6. Model reference adaptive control and adaptive stability augmentation

    DEFF Research Database (Denmark)

    Henningsen, Arne; Ravn, Ole

    1993-01-01

    A comparison of the standard concepts in MRAC design suggests that a combination of the implicit and the explicit design techniques may lead to an improvement of the overall system performance in the presence of unmodelled dynamics. Using the ideas of adaptive stability augmentation a combined...... stability augmented model reference design is proposed. By utilizing the closed-loop control error, a simple auxiliary controller is tuned, using a normalized MIT rule for the parameter adjustment. The MIT adjustment is protected against the effects of unmodelled dynamics by lowpass filtering...... of the gradient. The proposed method is verified through simulation results indicating that the method may lead to an improvement of the model reference controller in the presence of unmodelled dynamics...

  7. Output Feedback Stabilization with Nonlinear Predictive Control: Asymptotic properties

    Directory of Open Access Journals (Sweden)

    Lars Imsland

    2003-07-01

    Full Text Available State space based nonlinear model predictive control (NM PC needs the state for the prediction of the system behaviour. Unfortunately, for most applications, not all states are directly measurable. To recover the unmeasured states, typically a stable state observer is used. However, this implies that the stability of the closed-loop should be examined carefully, since no general nonlinear separation principle exists. Recently semi-global practical stability results for output feedback NMPC using a high-gain observer for state estimation have been established. One drawback of this result is that (in general the observer gain must be increased, if the desired set the state should converge to is made smaller. We show that under slightly stronger assumptions, not only practical stability, but also convergence of the system states and observer error to the origin for a sufficiently large but bounded observer gain can be achieved.

  8. Stability Boundaries for Offshore Wind Park Distributed Voltage Control

    DEFF Research Database (Denmark)

    Gryning, Mikkel P.S.; Wu, Qiuwei; Kocewiak, Lukasz

    2017-01-01

    pilot control. Using data from the actual wind power plant, all stabilizing subsystem voltage proportional-integral controller parameters are first characterized based on their Hurwitz signature. Inner loop current control is then designed using Internal Mode Control principles, and guidelines for feed......In order to identify mechanisms causing slow reactive power oscillations observed in an existing offshore wind power plant, and be able to avoid similar events in the future, voltage control is studied in this paper for a plant with a static synchronous compensator, type-4 wind turbines and a park...... forward filter design are given to obtain required disturbance rejection properties. The paper contributes by providing analytical relations between power plant control, droop, sampling time, electrical parameters and voltage control characteristics, and by assessing frequencies and damping of reactive...

  9. Transient stability improvement by nonlinear controllers based on tracking

    Energy Technology Data Exchange (ETDEWEB)

    Ramirez, Juan M. [Centro de Investigacion y Estudios Avanzados, Guadalajara, Mexico. Av. Cientifica 1145. Col. El Bajio. Zapopan, Jal. 45015 (Mexico); Arroyave, Felipe Valencia; Correa Gutierrez, Rosa Elvira [Universidad Nacional de Colombia, Sede Medellin. Facultad de Minas, Escuela de Mecatronica (Colombia)

    2011-02-15

    This paper deals with the control problem in multi-machine electric power systems, which represent complex great scale nonlinear systems. Thus, the controller design is a challenging problem. These systems are subjected to different perturbations, such as short circuits, connection and/or disconnection of loads, lines, or generators. Then, the utilization of controllers which guarantee good performance under those perturbations is required in order to provide electrical energy to the loads with admissible stability margins. The proposed controllers are based on a systematic strategy, which calculate nonlinear controllers for generating units in a power plant, both for voltage and velocity regulation. The formulation allows designing controllers in a multi-machine power system without intricate calculations. Results on a power system of the open research indicate the proposition's suitability. The problem is formulated as a tracking problem. The designed controllers may be implemented in any electric power system. (author)

  10. Discharge optimization and the control of edge stability

    International Nuclear Information System (INIS)

    Nave, M.F.F.; Lomas, P.J.; Huysmans, G.T.A.

    1999-01-01

    Discharge optimization for improving MHD stability of both core and edge was essential for the achievement of record fusion power discharges, in the ELM-free hot ion H mode regime, in the recent JET DT operation. The techniques used to increase edge stability are described. In particular the successful technique of current rampdown used to suppress the outer mode is reported. The increased stability of the outer mode by decreasing the edge current density confirms its identification as an n = 1 external kink. Decreasing the plasma current, however, decreases the ELM-free period, which is consistent with stability calculations that show an earlier onset of the ballooning limit. In order to increase external kink stability without causing a deterioration in the ELM-free period, a compromise was achieved by using plasma current rampdown, while working at the highest plasma current values possible. Results from a plasma current scan show that at the time of occurrence of the first giant ELM, the plasma stored energy, as well as the pressure measured at the top of the edge pedestal increase linearly with plasma current, for a given plasma configuration and power. This is consistent with models of the edge transport barrier, where the transport barrier width is proportional to the ion (or fast ion) poloidal Larmor radius. The MHD observations in DT and deuterium only discharges were found to be similar. Thus the experience gained on the control of MHD modes in deuterium plasmas could be fully exploited in the DT campaign. (author)

  11. Control Design of a Nonlinear Controller to Stabilize the Nonlinear ...

    African Journals Online (AJOL)

    inyangs

    protocol employed to combat the problem of proper queue utilization, busty packet drop and adaptable delay. TCP .... This case considers a delay on the control input only and the link capacity is made to be very large to ... We take the derivative of equation (13) with respect to t and apply the Leibniz integrator rule to obtain.

  12. Stability and synchronization control of stochastic neural networks

    CERN Document Server

    Zhou, Wuneng; Zhou, Liuwei; Tong, Dongbing

    2016-01-01

    This book reports on the latest findings in the study of Stochastic Neural Networks (SNN). The book collects the novel model of the disturbance driven by Levy process, the research method of M-matrix, and the adaptive control method of the SNN in the context of stability and synchronization control. The book will be of interest to university researchers, graduate students in control science and engineering and neural networks who wish to learn the core principles, methods, algorithms and applications of SNN.

  13. Non-catalytic Roles for XPG with BRCA1 and BRCA2 in Homologous Recombination and Genome Stability

    DEFF Research Database (Denmark)

    Trego, Kelly S.; Groesser, Torsten; Davalos, Albert R.

    2016-01-01

    XPG is a structure-specific endonuclease required for nucleotide excision repair, and incision-defective XPG mutations cause the skin cancer-prone syndrome xeroderma pigmentosum. Truncating mutations instead cause the neurodevelopmental progeroid disorder Cockayne syndrome, but little is known ab......-phosphorylation and persistent chromatin binding. These unexpected findings establish XPG as an HRR protein with important roles in genome stability and suggest how XPG defects produce severe clinical consequences including cancer and accelerated aging....... about how XPG loss results in this devastating disease. We identify XPG as a partner of BRCA1 and BRCA2 in maintaining genomic stability through homologous recombination (HRR). XPG depletion causes DNA double-strand breaks, chromosomal abnormalities, cell-cycle delays, defective HRR, inability...

  14. Stabilization of the genome of the mismatch repair deficient Mycobacterium tuberculosis by context-dependent codon choice.

    Science.gov (United States)

    Wanner, Roger M; Güthlein, Carolin; Springer, Burkhard; Böttger, Erik C; Ackermann, Martin

    2008-05-28

    The rate at which a stretch of DNA mutates is determined by the cellular systems for DNA replication and repair, and by the nucleotide sequence of the stretch itself. One sequence feature with a particularly strong influence on the mutation rate are nucleotide repeats. Some microbial pathogens use nucleotide repeats in their genome to stochastically vary phenotypic traits and thereby evade host defense. However, such unstable sequences also come at a cost, as mutations are often deleterious. Here, we analyzed how these opposing forces shaped genome stability in the human pathogen Mycobacterium tuberculosis. M. tuberculosis lacks a mismatch repair system, and this renders nucleotide repeats particularly unstable. We found that proteins of M. tuberculosis are encoded by using codons in a context-dependent manner that prevents the emergence of nucleotide repeats. This context-dependent codon choice leads to a strong decrease in the estimated frame-shift mutation rate and thus to an increase in genome stability. These results indicate that a context-specific codon choice can partially compensate for the lack of a mismatch repair system, and helps to maintain genome integrity in this pathogen.

  15. Stabilization of the genome of the mismatch repair deficient Mycobacterium tuberculosis by context-dependent codon choice

    Directory of Open Access Journals (Sweden)

    Ackermann Martin

    2008-05-01

    Full Text Available Abstract Background The rate at which a stretch of DNA mutates is determined by the cellular systems for DNA replication and repair, and by the nucleotide sequence of the stretch itself. One sequence feature with a particularly strong influence on the mutation rate are nucleotide repeats. Some microbial pathogens use nucleotide repeats in their genome to stochastically vary phenotypic traits and thereby evade host defense. However, such unstable sequences also come at a cost, as mutations are often deleterious. Here, we analyzed how these opposing forces shaped genome stability in the human pathogen Mycobacterium tuberculosis. M. tuberculosis lacks a mismatch repair system, and this renders nucleotide repeats particularly unstable. Results We found that proteins of M. tuberculosis are encoded by using codons in a context-dependent manner that prevents the emergence of nucleotide repeats. This context-dependent codon choice leads to a strong decrease in the estimated frame-shift mutation rate and thus to an increase in genome stability. Conclusion These results indicate that a context-specific codon choice can partially compensate for the lack of a mismatch repair system, and helps to maintain genome integrity in this pathogen.

  16. New aspects of protein stability and turnover in the regulation of genome integrity

    DEFF Research Database (Denmark)

    Gallina, Irene

    of DNA repair is the control of protein abundance, both at a global cellular level, and locally at the site of damage. This is achieved through transcriptional regulation of protein synthesis and through the control of protein stability and turnover. In this study, we investigate the role of Rad56...... sensitivity when mutant. Prior to the work presented here,all these loci have been mapped to a specific gene except RAD56. We map the rad56-1 mutation to the NAT3 gene, which encodes the catalytic subunit of the NatB N-terminal acetyltransferase in yeast. Deletion of RAD56 causes sensitivity to X-rays, methyl......-scale studies investigating factors involved in DNA metabolism, but no specific function has been assigned to Cmr1. Taking advantage of a series of high-throughput screens we characterize Cmr1 as a chromatinassociated protein, involved in the regulation of fork progression in the presence of replication stress...

  17. Stability control of a flexible maneuverable tethered space net robot

    Science.gov (United States)

    Zhang, Fan; Huang, Panfeng

    2018-04-01

    As a promising solution for active space debris capture and removal, a maneuverable Tethered Space Net Robot (TSNR) is proposed as an improved Space Tethered Net (TSN). In addition to the advantages inherit to the TSN, the TSNR's maneuverability expands the capture's potential. However, oscillations caused by the TSNR's flexibility and elasticity of make higher requests of the control scheme. Based on the dynamics model, a modified adaptive super-twisting sliding mode control scheme is proposed in this paper for TSNR stability control. The proposed continuous control force can effectively suppress oscillations. Theoretical verification and numerical simulations demonstrate that the desired trajectory can be tracked steadily and efficiently by employing the proposed control scheme.

  18. Designing hybrid grass genomes to control runoff generation

    Science.gov (United States)

    MacLeod, C.; Binley, A.; Humphreys, M.; King, I. P.; O'Donovan, S.; Papadopoulos, A.; Turner, L. B.; Watts, C.; Whalley, W. R.; Haygarth, P.

    2010-12-01

    Sustainable management of water in landscapes requires balancing demands of agricultural production whilst moderating downstream effects like flooding. Pasture comprises 69% of global agricultural areas and is essential for producing food and fibre alongside environmental goods and services. Thus there is a need to breed forage grasses that deliver multiple benefits through increased levels of productivity whilst moderating fluxes of water. Here we show that a novel grass hybrid that combines the entire genomes of perennial ryegrass (Lolium perenne - the grass of choice for Europe’s forage agriculture) and meadow fescue (Festuca pratensis) has a significant role in flood prevention. Field plot experiments established differences in runoff generation with the hybrid cultivar reducing runoff by 50% compared to perennial ryegrass cultivar, and by 35% compared to a meadow fescue cultivar (34 events over two years, replicated randomized-block design, statistically significant differences). This important research outcome was the result of a project that combined plant genetics, soil physics and plot scale hydrology to identify novel grass genotypes that can reduce runoff from grassland systems. Through a coordinated series of experiments examining effects from the gene to plot scale, we have identified that the rapid growth and then turnover of roots in the L. perenne x F. pratensis hybrid is likely to be a key mechanism in reducing runoff generation. More broadly this is an exciting first step to realizing the potential to design grass genomes to achieve both food production, and to deliver flood control, a key ecosystem service.

  19. Genome-health nutrigenomics and nutrigenetics: nutritional requirements or 'nutriomes' for chromosomal stability and telomere maintenance at the individual level.

    Science.gov (United States)

    Bull, Caroline; Fenech, Michael

    2008-05-01

    It is becoming increasingly evident that (a) risk for developmental and degenerative disease increases with more DNA damage, which in turn is dependent on nutritional status, and (b) the optimal concentration of micronutrients for prevention of genome damage is also dependent on genetic polymorphisms that alter the function of genes involved directly or indirectly in the uptake and metabolism of micronutrients required for DNA repair and DNA replication. The development of dietary patterns, functional foods and supplements that are designed to improve genome-health maintenance in individuals with specific genetic backgrounds may provide an important contribution to an optimum health strategy based on the diagnosis and individualised nutritional prevention of genome damage, i.e. genome health clinics. The present review summarises some of the recent knowledge relating to micronutrients that are associated with chromosomal stability and provides some initial insights into the likely nutritional factors that may be expected to have an impact on the maintenance of telomeres. It is evident that developing effective strategies for defining nutrient doses and combinations or 'nutriomes' for genome-health maintenance at the individual level is essential for further progress in this research field.

  20. Stability Concerns for Indirect Consumer Control in Smart Grids

    DEFF Research Database (Denmark)

    Juelsgaard, Morten; Andersen, Palle; Wisniewski, Rafal

    2013-01-01

    by an external third party, and indirect consumer control through incentives and price signals. In this work we present a simple formulation of indirect control, where the behavior of each consumer, is governed by local optimization of energy consumption. The local optimization accounts for both cost of energy...... and distribution losses, as well as any discomfort incurred by consumers from any shift in energy consumption. Our work will illustrate that in the simplest formulation of indirect control, the stability is greatly affected of both the behavior of consumers, and the number of consumers to include. We will show how......Demand side management will be an important tool for maintaining a balanced electrical grid in the future, when the penetration of volatile resources, such as wind and solar energy increases. Recent research focuses on two different management approaches, namely direct consumer control...

  1. Stability of Closed Loop Controlled Repetitive Periodic System applied to control of CD-Player

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Stoustrup, Jakob; Andersen, Palle

    2005-01-01

    In this paper a criterion for stability of specific control scheme for handling linear dynamic control systems with repetitive periodic sensor faults is derived. The given system and control scheme are described and defined. By combining these with the lifting technique a necessary and sufficient...

  2. Hybrid AC-High Voltage DC Grid Stability and Controls

    Science.gov (United States)

    Yu, Jicheng

    The growth of energy demands in recent years has been increasing faster than the expansion of transmission facility construction. This tendency cooperating with the continuous investing on the renewable energy resources drives the research, development, and construction of HVDC projects to create a more reliable, affordable, and environmentally friendly power grid. Constructing the hybrid AC-HVDC grid is a significant move in the development of the HVDC techniques; the form of dc system is evolving from the point-to-point stand-alone dc links to the embedded HVDC system and the multi-terminal HVDC (MTDC) system. The MTDC is a solution for the renewable energy interconnections, and the MTDC grids can improve the power system reliability, flexibility in economic dispatches, and converter/cable utilizing efficiencies. The dissertation reviews the HVDC technologies, discusses the stability issues regarding the ac and HVDC connections, proposes a novel power oscillation control strategy to improve system stability, and develops a nonlinear voltage droop control strategy for the MTDC grid. To verify the effectiveness the proposed power oscillation control strategy, a long distance paralleled AC-HVDC transmission test system is employed. Based on the PSCAD/EMTDC platform simulation results, the proposed power oscillation control strategy can improve the system dynamic performance and attenuate the power oscillations effectively. To validate the nonlinear voltage droop control strategy, three droop controls schemes are designed according to the proposed nonlinear voltage droop control design procedures. These control schemes are tested in a hybrid AC-MTDC system. The hybrid AC-MTDC system, which is first proposed in this dissertation, consists of two ac grids, two wind farms and a five-terminal HVDC grid connecting them. Simulation studies are performed in the PSCAD/EMTDC platform. According to the simulation results, all the three design schemes have their unique salient

  3. Stability analysis of multiple-robot control systems

    Science.gov (United States)

    Wen, John T.; Kreutz, Kenneth

    1989-01-01

    In a space telerobotic service scenario, cooperative motion and force control of multiple robot arms are of fundamental importance. Three paradigms to study this problem are proposed. They are distinguished by the set of variables used for control design. They are joint torques, arm tip force vectors, and an accelerated generalized coordinate set. Control issues related to each case are discussed. The latter two choices require complete model information, which presents practical modeling, computational, and robustness problems. Therefore, focus is on the joint torque control case to develop relatively model independent motion and internal force control laws. The rigid body assumption allows the motion and force control problems to be independently addressed. By using an energy motivated Lyapunov function, a simple proportional derivative plus gravity compensation type of motion control law is always shown to be stabilizing. The asymptotic convergence of the tracing error to zero requires the use of a generalized coordinate with the contact constraints taken into account. If a non-generalized coordinate is used, only convergence to a steady state manifold can be concluded. For the force control, both feedforward and feedback schemes are analyzed. The feedback control, if proper care has been taken, exhibits better robustness and transient performance.

  4. A fuzzy logic pitch angle controller for power system stabilization

    Energy Technology Data Exchange (ETDEWEB)

    Jauch, Clemens; Cronin, Tom; Sorensen, Poul [Wind Energy Department, Riso National Laboratory, PO Box 49, DK-4000 Roskilde, (Denmark); Jensen, Birgitte Bak [Institute of Energy Technology, Aalborg University, Pontoppidanstraede 101, DK-9220 Aalborg East, (Denmark)

    2006-07-12

    In this article the design of a fuzzy logic pitch angle controller for a fixed speed, active-stall wind turbine, which is used for power system stabilization, is presented. The system to be controlled, which is the wind turbine and the power system to which the turbine is connected, is described. The advantages of fuzzy logic control when applied to large-signal control of active-stall wind turbines are outlined. The general steps of the design process for a fuzzy logic controller, including definition of the controller inputs, set-up of the fuzzy rules and the method of defuzzification, are described. The performance of the controller is assessed by simulation, where the wind turbine's task is to dampen power system oscillations. In the scenario simulated for this work, the wind turbine has to ride through a transient short-circuit fault and subsequently contribute to the damping of the grid frequency oscillations that are caused by the transient fault. It is concluded that the fuzzy logic controller enables the wind turbine to dampen power system oscillations. It is also concluded that, owing to the inherent non-linearities in a wind turbine and the unpredictability of the whole system, the fuzzy logic controller is very suitable for this application. (Author).

  5. LOFT pump speed controller stability and accuracy analysis

    International Nuclear Information System (INIS)

    Good, R.R.

    1978-01-01

    Two system modifications to the primary coolant pumps motor generators control systems have recently been completed. The range of pump speed operation has been extended and the scoop tube positioner motor replaced. This has necessitated a re-analysis of PSMG stability throughout its range of operation. System accuracy requirements of less than 4 Hz differential pump speed when operating at less than 35 Hz and 8.5 Hz differential pump speed when operating at greater than 35 Hz can be guaranteed by specifying the gain of the system. The installation of the new scoop tube positioner motor will increase the PSMG system's bandwidth and stability. Low speed pump trips should be carefully evaluated if the pump's operational range is to extend to 10 Hz

  6. Stability of Genome Composition and Recombination between Homoeologous Chromosomes in Festulolium (Festuca × Lolium) Cultivars

    Czech Academy of Sciences Publication Activity Database

    Kopecký, David; Šimoníková, Denisa; Ghesquière, M.; Doležel, Jaroslav

    2017-01-01

    Roč. 151, č. 2 (2017), s. 106-114 ISSN 1424-8581 R&D Projects: GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : Festulolium * Genome composition * Genomic in situ hybridization * Grass hybrids * Homoeologous recombination * Lolium × Festuca Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Plant sciences, botany Impact factor: 1.354, year: 2016

  7. Steam Turbine Control Valve Stiction Effect on Power System Stability

    International Nuclear Information System (INIS)

    Halimi, B.

    2010-01-01

    One of the most important problems in power system dynamic stability is low frequency oscillations. This kind of oscillation has significant effects on the stability and security of the power system. In some previous papers, a fact was introduced that a steam pressure continuous fluctuation in turbine steam inlet pipeline may lead to a kind of low frequency oscillation of power systems. Generally, in a power generation plant, steam turbine system composes of some main components, i.e. a boiler or steam generator, stop valves, control valves and turbines that are connected by piping. In the conventional system, the turbine system is composed with a lot of stop and control valves. The steam is provided by a boiler or steam generator. In an abnormal case, the stop valve shuts of the steal flow to the turbine. The steam flow to the turbine is regulated by controlling the control valves. The control valves are provided to regulate the flow of steam to the turbine for starting, increasing or decreasing the power, and also maintaining speed control with the turbine governor system. Unfortunately, the control valve has inherent static friction (stiction) nonlinearity characteristics. Industrial surveys indicated that about 20-30% of all control loops oscillate due to valve problem caused by this nonlinear characteristic. In this paper, steam turbine control valve stiction effect on power system oscillation is presented. To analyze the stiction characteristic effect, firstly a model of control valve and its stiction characteristic are derived by using Newton's laws. A complete tandem steam prime mover, including a speed governing system, a four-stage steam turbine, and a shaft with up to for masses is adopted to analyze the performance of the steam turbine. The governor system consists of some important parts, i.e. a proportional controller, speed relay, control valve with its stiction characteristic, and stem lift position of control valve controller. The steam turbine has

  8. Stability and Bifurcation in Magnetic Flux Feedback Maglev Control System

    Directory of Open Access Journals (Sweden)

    Wen-Qing Zhang

    2013-01-01

    Full Text Available Nonlinear properties of magnetic flux feedback control system have been investigated mainly in this paper. We analyzed the influence of magnetic flux feedback control system on control property by time delay and interfering signal of acceleration. First of all, we have established maglev nonlinear model based on magnetic flux feedback and then discussed hopf bifurcation’s condition caused by the acceleration’s time delay. The critical value of delayed time is obtained. It is proved that the period solution exists in maglev control system and the stable condition has been got. We obtained the characteristic values by employing center manifold reduction theory and normal form method, which represent separately the direction of hopf bifurcation, the stability of the period solution, and the period of the period motion. Subsequently, we discussed the influence maglev system on stability of by acceleration’s interfering signal and obtained the stable domain of interfering signal. Some experiments have been done on CMS04 maglev vehicle of National University of Defense Technology (NUDT in Tangshan city. The results of experiments demonstrate that viewpoints of this paper are correct and scientific. When time lag reaches the critical value, maglev system will produce a supercritical hopf bifurcation which may cause unstable period motion.

  9. Microgrid Stability Controller Based on Adaptive Robust Total SMC

    Directory of Open Access Journals (Sweden)

    Xiaoling Su

    2015-03-01

    Full Text Available This paper presents a microgrid stability controller (MSC in order to provide existing distributed generation units (DGs the additional functionality of working in islanding mode without changing their control strategies in grid-connected mode and to enhance the stability of the microgrid. Microgrid operating characteristics and mathematical models of the MSC indicate that the system is inherently nonlinear and time-variable. Therefore, this paper proposes an adaptive robust total sliding-mode control (ARTSMC system for the MSC. It is proved that the ARTSMC system is insensitive to parametric uncertainties and external disturbances. The MSC provides fast dynamic response and robustness to the microgrid. When the system is operating in grid-connected mode, it is able to improve the controllability of the exchanged power between the microgrid and the utility grid, while smoothing the DGs’ output power. When the microgrid is operating in islanded mode, it provides voltage and frequency support, while guaranteeing seamless transition between the two operation modes. Simulation and experimental results show the effectiveness of the proposed approach.

  10. Biological stability of drinking water: controlling factors, methods and challenges

    Directory of Open Access Journals (Sweden)

    Emmanuelle ePrest

    2016-02-01

    Full Text Available Biological stability of drinking water refers to the concept of providing consumers with drinking water of same microbial quality at the tap as produced at the water treatment facility. However, uncontrolled growth of bacteria can occur during distribution in water mains and premise plumbing, and can lead to hygienic (e.g. development of opportunistic pathogens, aesthetic (e.g. deterioration of taste, odour, colour or operational (e.g. fouling or biocorrosion of pipes problems. Drinking water contains diverse microorganisms competing for limited available nutrients for growth. Bacterial growth and interactions are regulated by factors such as (i type and concentration of available organic and inorganic nutrients, (ii type and concentration of residual disinfectant, (iii presence of predators such as protozoa and invertebrates, (iv environmental conditions such as water temperature, and (v spatial location of microorganisms (bulk water, sediment or biofilm. Water treatment and distribution conditions in water mains and premise plumbing affect each of these factors and shape bacterial community characteristics (abundance, composition, viability in distribution systems. Improved understanding of bacterial interactions in distribution systems and of environmental conditions impact is needed for better control of bacterial communities during drinking water production and distribution. This article reviews (i existing knowledge on biological stability controlling factors and (ii how these factors are affected by drinking water production and distribution conditions. In addition, (iii the concept of biological stability is discussed in light of experience with well-established and new analytical methods, enabling high throughput analysis and in-depth characterization of bacterial communities in drinking water. We discuss how knowledge gained from novel techniques will improve design and monitoring of water treatment and distribution systems in order to

  11. Biological Stability of Drinking Water: Controlling Factors, Methods, and Challenges

    Science.gov (United States)

    Prest, Emmanuelle I.; Hammes, Frederik; van Loosdrecht, Mark C. M.; Vrouwenvelder, Johannes S.

    2016-01-01

    Biological stability of drinking water refers to the concept of providing consumers with drinking water of same microbial quality at the tap as produced at the water treatment facility. However, uncontrolled growth of bacteria can occur during distribution in water mains and premise plumbing, and can lead to hygienic (e.g., development of opportunistic pathogens), aesthetic (e.g., deterioration of taste, odor, color) or operational (e.g., fouling or biocorrosion of pipes) problems. Drinking water contains diverse microorganisms competing for limited available nutrients for growth. Bacterial growth and interactions are regulated by factors, such as (i) type and concentration of available organic and inorganic nutrients, (ii) type and concentration of residual disinfectant, (iii) presence of predators, such as protozoa and invertebrates, (iv) environmental conditions, such as water temperature, and (v) spatial location of microorganisms (bulk water, sediment, or biofilm). Water treatment and distribution conditions in water mains and premise plumbing affect each of these factors and shape bacterial community characteristics (abundance, composition, viability) in distribution systems. Improved understanding of bacterial interactions in distribution systems and of environmental conditions impact is needed for better control of bacterial communities during drinking water production and distribution. This article reviews (i) existing knowledge on biological stability controlling factors and (ii) how these factors are affected by drinking water production and distribution conditions. In addition, (iii) the concept of biological stability is discussed in light of experience with well-established and new analytical methods, enabling high throughput analysis and in-depth characterization of bacterial communities in drinking water. We discussed, how knowledge gained from novel techniques will improve design and monitoring of water treatment and distribution systems in order

  12. Biological Stability of Drinking Water: Controlling Factors, Methods, and Challenges

    KAUST Repository

    Prest, Emmanuelle I.

    2016-02-01

    Biological stability of drinking water refers to the concept of providing consumers with drinking water of same microbial quality at the tap as produced at the water treatment facility. However, uncontrolled growth of bacteria can occur during distribution in water mains and premise plumbing, and can lead to hygienic (e.g., development of opportunistic pathogens), aesthetic (e.g., deterioration of taste, odor, color) or operational (e.g., fouling or biocorrosion of pipes) problems. Drinking water contains diverse microorganisms competing for limited available nutrients for growth. Bacterial growth and interactions are regulated by factors, such as (i) type and concentration of available organic and inorganic nutrients, (ii) type and concentration of residual disinfectant, (iii) presence of predators, such as protozoa and invertebrates, (iv) environmental conditions, such as water temperature, and (v) spatial location of microorganisms (bulk water, sediment, or biofilm). Water treatment and distribution conditions in water mains and premise plumbing affect each of these factors and shape bacterial community characteristics (abundance, composition, viability) in distribution systems. Improved understanding of bacterial interactions in distribution systems and of environmental conditions impact is needed for better control of bacterial communities during drinking water production and distribution. This article reviews (i) existing knowledge on biological stability controlling factors and (ii) how these factors are affected by drinking water production and distribution conditions. In addition, (iii) the concept of biological stability is discussed in light of experience with well-established and new analytical methods, enabling high throughput analysis and in-depth characterization of bacterial communities in drinking water. We discussed, how knowledge gained from novel techniques will improve design and monitoring of water treatment and distribution systems in order

  13. Preliminary Experimental Analysis of Soil Stabilizers for Contamination Control

    International Nuclear Information System (INIS)

    Lagos, L.; Varona, J.; Zidan, A.; Gudavalli, R.; Wu, Kuang-His

    2006-01-01

    A major focus of Department of Energy's (DOE's) environmental management mission at the Hanford site involves characterizing and remediating contaminated soil and groundwater; stabilizing contaminated soil; remediating disposal sites; decontaminating and decommissioning structures, and demolishing former plutonium production process buildings, nuclear reactors, and separation plants; maintaining inactive waste sites; transitioning facilities into the surveillance and maintenance program; and mitigating effects to biological and cultural resources from site development and environmental cleanup and restoration activities. For example, a total of 470,914 metric tons of contaminated soil from 100 Areas remediation activities were disposed at the Environmental Restoration Disposal Facility (ERDF) during 2004. The Applied Research Center (ARC) at Florida International University (FIU) is supporting the Hanford's site remediation program by analyzing the effectiveness of several soil stabilizers (fixatives) for contamination control during excavation activities. The study is focusing on determining the effects of varying soil conditions, temperature, humidity and wind velocity on the effectiveness of the candidate stabilizers. The test matrix consists of a soil penetration-depth study, wind tunnel experiments for determination of threshold velocity, and temperature and moisture-controlled drying/curing experiments. These three set of experiments are designed to verify performance metrics, as well as provide insight into what fundamental forces are altered by the use of the stabilizer. This paper only presents the preliminary results obtained during wind tunnel experiments using dry Hanford soil samples (with 2.7% moisture by weight). These dry soil samples were exposed to varying wind speeds from 2.22 m/sec to 8.88 m/sec. Furthermore, airborne particulate data was collected for the dry Hanford soil experiments using an aerosol analyzer instrument. (authors)

  14. Genomes

    National Research Council Canada - National Science Library

    Brown, T. A. (Terence A.)

    2002-01-01

    ... of genome expression and replication processes, and transcriptomics and proteomics. This text is richly illustrated with clear, easy-to-follow, full color diagrams, which are downloadable from the book's website...

  15. Sliding Mode Controller and Lyapunov Redesign Controller to Improve Microgrid Stability

    DEFF Research Database (Denmark)

    Hossain, Eklas; Perez, Ron; Padmanaban, Sanjeevikumar

    2017-01-01

    technique is used to enhance stability of microgrids. Besides adopting this technique here, Sliding Mode Controller (SMC) and Lyapunov Redesign Controller (LRC), two of the most prominent nonlinear control techniques, are individually implemented to control microgrid system stability with desired robustness....... CPL power is then varied to compare robustness of these two control techniques. This investigation revealed the better performance of the LRC system compared to SMC to retain stability in microgrid with dense CPL load. All the necessary results are simulated in Matlab/Simulink platform for authentic......To mitigate the microgrid instability despite the presence of dense Constant Power Load (CPL) loads in the system, a number of compensation techniques have already been gone through extensive research, proposed, and implemented around the world. In this paper, a storage based load side compensation...

  16. Effectiveness of electronic stability control on single-vehicle accidents

    DEFF Research Database (Denmark)

    Lyckegaard, Allan; Hels, Tove; Bernhoft, Inger Marie

    2015-01-01

    the injury severity categories (slight, severe, and fatal). Conclusions: In line with previous results, this study concludes that ESC reduces the risk for single-vehicle injury accidents by 31% when controlling for various confounding factors related to the driver, the car, and the accident surroundings......Objective: This study aims at evaluating the effectiveness of electronic stability control (ESC) on single-vehicle injury accidents while controlling for a number of confounders influencing the accident risk. Methods: Using police-registered injury accidents from 2004 to 2011 in Denmark with cars...... the following were significant. For the driver: Age, gender, driving experience, valid driving license, and seat belt use. For the vehicle: Year of registration, weight, and ESC. For the accident surroundings: Visibility, light, and location. Finally, for the road: Speed limit, surface, and section...

  17. Optimal Control of a Ballast-Stabilized Floating Wind Turbine

    DEFF Research Database (Denmark)

    Christiansen, Søren; Knudsen, Torben; Bak, Thomas

    2011-01-01

    Offshore wind energy capitalizes on the higher and less turbulent wind speeds at sea. The use of floating structures for deeper waters is being explored. The control objective is a tradeoff between power capture and fatigue, especially that produced by the oscillations caused by the reduced...... structural stiffness of a floating installation in combination with a coupling between the fore–aft motion of the tower and the blade pitch. To address this problem, the present paper models a ballast-stabilized floating wind turbine, and suggests a linear quadratic regulator (LQR) in combination with a wind...... estimator and a state observer. The results are simulated using aero elastic code and analysed in terms of damage equivalent loads. When compared to a baseline controller, this controller clearly demonstrates better generator speed and power tracking while reducing fatigue loads....

  18. Movement augmentation to evaluate human control of locomotor stability.

    Science.gov (United States)

    Brown, Geoffrey; Wu, Mengnan Mary; Huang, Felix C; Gordon, Keith E

    2017-07-01

    Controlling center of mass (COM) position and velocity within a dynamic base of support is essential for gait stability. This skill is often compromised following neurologic injury, creating a need to develop effective interventions to enhance gait stability. A movement augmentation paradigm applied to walking could potentially be used to improve control of COM dynamics. We have developed a cable robot system, the Agility Trainer, to apply continuous frontal-plane forces to the pelvis during treadmill walking. This cable robot system uses a set of series elastic actuators powered by linear motors to create bilateral forces. Here we use the Agility Trainer to create a negative viscosity force field proportional to the subject's lateral velocity. Two healthy young subjects performed two 10-minute walking trials, Baseline and Negative Viscosity. During the first minute of walking in the Negative Viscosity field, participants' lateral COM motion became less controlled when compared to the rhythmic sinusoidal motion observed during Baseline walking. By the 10th minute of walking in the Negative Viscosity field the participants had adapted their gait patterns, decreasing their variation in peak lateral COM speed each stride. These results demonstrate that it is feasible to use the Agility Trainer to apply a movement augmentation paradigm to human walking.

  19. Stability and vibration control in synchrotron light source buildings

    Energy Technology Data Exchange (ETDEWEB)

    Godel, J.B.

    1991-01-01

    Synchrotron light sources have undergone three generations of development in the last two decades. The National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory has two second generation'' storage rings that currently provide the world's most intense sources of photons in the VUV and X-ray spectral ranges. There are almost 90 beam lines serving a community of 2600 scientists from 370 institutions. They are engaged in basic and applied research in physics, chemistry, biology, medicine, materials science and various technologies. When design of the NSLS began in 1977, emphasis was given to the stability of the concrete slab on which the storage rings and experimental beam lines were placed. Stability is the result of controlling: vibration from sources internal and external to the building, thermal effects of air and water temperature variations, foundation settlement and contact between the slab and underlying subsoil. With the advent of new research where highly focused beams of x-rays must be placed on increasingly smaller targets located 35 meters or more from the source, and the development of x-ray lithography with resolutions approaching 0.1 micron at chip exposure stations, even greater attention to stability is required in building designs. This paper will review the results of the successful NSLS experience and give an integrated design approach that includes elements which contribute to instabilities, and the means available to reduce them to acceptable levels.

  20. Stability and vibration control in synchrotron light source buildings

    Energy Technology Data Exchange (ETDEWEB)

    Godel, J.B.

    1991-12-31

    Synchrotron light sources have undergone three generations of development in the last two decades. The National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory has two ``second generation`` storage rings that currently provide the world`s most intense sources of photons in the VUV and X-ray spectral ranges. There are almost 90 beam lines serving a community of 2600 scientists from 370 institutions. They are engaged in basic and applied research in physics, chemistry, biology, medicine, materials science and various technologies. When design of the NSLS began in 1977, emphasis was given to the stability of the concrete slab on which the storage rings and experimental beam lines were placed. Stability is the result of controlling: vibration from sources internal and external to the building, thermal effects of air and water temperature variations, foundation settlement and contact between the slab and underlying subsoil. With the advent of new research where highly focused beams of x-rays must be placed on increasingly smaller targets located 35 meters or more from the source, and the development of x-ray lithography with resolutions approaching 0.1 micron at chip exposure stations, even greater attention to stability is required in building designs. This paper will review the results of the successful NSLS experience and give an integrated design approach that includes elements which contribute to instabilities, and the means available to reduce them to acceptable levels.

  1. Stability and vibration control in synchrotron light source buildings

    International Nuclear Information System (INIS)

    Godel, J.B.

    1991-01-01

    Synchrotron light sources have undergone three generations of development in the last two decades. The National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory has two ''second generation'' storage rings that currently provide the world's most intense sources of photons in the VUV and X-ray spectral ranges. There are almost 90 beam lines serving a community of 2600 scientists from 370 institutions. They are engaged in basic and applied research in physics, chemistry, biology, medicine, materials science and various technologies. When design of the NSLS began in 1977, emphasis was given to the stability of the concrete slab on which the storage rings and experimental beam lines were placed. Stability is the result of controlling: vibration from sources internal and external to the building, thermal effects of air and water temperature variations, foundation settlement and contact between the slab and underlying subsoil. With the advent of new research where highly focused beams of x-rays must be placed on increasingly smaller targets located 35 meters or more from the source, and the development of x-ray lithography with resolutions approaching 0.1 micron at chip exposure stations, even greater attention to stability is required in building designs. This paper will review the results of the successful NSLS experience and give an integrated design approach that includes elements which contribute to instabilities, and the means available to reduce them to acceptable levels

  2. Plasma control system upgrade and increased plasma stability in NSTX

    Energy Technology Data Exchange (ETDEWEB)

    Mastrovito, D., E-mail: dmastrovito@pppl.go [Princeton Plasma Physics Laboratory, P.O. Box 451 Princeton, NJ 08543 (United States); Gates, D.; Gerhard, S.; Lawson, J.; Ludescher-Furth, C.; Marsala, R. [Princeton Plasma Physics Laboratory, P.O. Box 451 Princeton, NJ 08543 (United States)

    2010-07-15

    Plasma control on the National Spherical Torus Experiment (NSTX) was previously accomplished using eight 333 MHz G4 processors built by Sky computers. Several planned improvements and additional control algorithms required significant upgrades to our real-time control computers and real-time data acquisition infrastructure. Several in-house modules have been designed and implemented including: the digital time stamp module (DITS) and for digital/analog front panel data port (FPDP) output, the FPDP output module digital/analog (FOMD/A). Standard Linux based Intel computers perform the real-time control tasks and InfiniBand as been employed for communication between a user-accessible 'host' server and the real-time computer. In addition to several independent real-time processes the General Atomics developed PCS (Bell (2006) ) system infrastructure continues to be used on NSTX. While maintaining previous functionality, improvements in the control system software include: an RWM feedback algorithm, beta feedback NBI control, more comprehensive error logging and trapping, more user-friendly interface, more complete archiving and restoring functionality, and better status reporting and diagnostic tools. Once completed, we succeeded in increasing overall plasma stability and decreasing control system latency by several times.

  3. Handbook of electrical power system dynamics modeling, stability, and control

    CERN Document Server

    Eremia, Mircea

    2013-01-01

    Complete guidance for understanding electrical power system dynamics and blackouts This handbook offers a comprehensive and up-to-date treatment of power system dynamics. Addressing the full range of topics, from the fundamentals to the latest technologies in modeling, stability, and control, Handbook of Electrical Power System Dynamics provides engineers with hands-on guidance for understanding the phenomena leading to blackouts so they can design the most appropriate solutions for a cost-effective and reliable operation. Focusing on system dynamics, the book details

  4. Burn Control in Fusion Reactors via Nonlinear Stabilization Techniques

    International Nuclear Information System (INIS)

    Schuster, Eugenio; Krstic, Miroslav; Tynan, George

    2003-01-01

    Control of plasma density and temperature magnitudes, as well as their profiles, are among the most fundamental problems in fusion reactors. Existing efforts on model-based control use control techniques for linear models. In this work, a zero-dimensional nonlinear model involving approximate conservation equations for the energy and the densities of the species was used to synthesize a nonlinear feedback controller for stabilizing the burn condition of a fusion reactor. The subignition case, where the modulation of auxiliary power and fueling rate are considered as control forces, and the ignition case, where the controlled injection of impurities is considered as an additional actuator, are treated separately.The model addresses the issue of the lag due to the finite time for the fresh fuel to diffuse into the plasma center. In this way we make our control system independent of the fueling system and the reactor can be fed either by pellet injection or by puffing. This imposed lag is treated using nonlinear backstepping.The nonlinear controller proposed guarantees a much larger region of attraction than the previous linear controllers. In addition, it is capable of rejecting perturbations in initial conditions leading to both thermal excursion and quenching, and its effectiveness does not depend on whether the operating point is an ignition or a subignition point.The controller designed ensures setpoint regulation for the energy and plasma parameter β with robustness against uncertainties in the confinement times for different species. Hence, the controller can increase or decrease β, modify the power, the temperature or the density, and go from a subignition to an ignition point and vice versa

  5. Co-simulation of heavy truck tire dynamics and electronic stability control systems (phase A).

    Science.gov (United States)

    2009-07-01

    Electronic stability control (ESC) systems have been proven to be an effective means of preventing instability and loss of control on both passenger vehicles and heavy trucks. In addition, roll stability algorithms are an effective means of reducing ...

  6. The problem of the driverless vehicle specified path stability control

    Science.gov (United States)

    Buznikov, S. E.; Endachev, D. V.; Elkin, D. S.; Strukov, V. O.

    2018-02-01

    Currently the effort of many leading foreign companies is focused on creation of driverless transport for transportation of cargo and passengers. Among many practical problems arising while creating driverless vehicles, the problem of the specified path stability control occupies a central place. The purpose of this paper is formalization of the problem in question in terms of the quadratic functional of the control quality, the comparative analysis of the possible solutions and justification of the choice of the optimum technical solution. As square value of the integral of the deviation from the specified path is proposed as the quadratic functional of the control quality. For generation of the set of software and hardware solution variants the Zwicky “morphological box” method is used within the hardware and software environments. The heading control algorithms use the wheel steering angle data and the deviation from the lane centerline (specified path) calculated based on the navigation data and the data from the video system. Where the video system does not detect the road marking, the control is carried out based on the wheel navigation system data and where recognizable road marking exits - based on to the video system data. The analysis of the test results allows making the conclusion that the application of the combined navigation system algorithms that provide quasi-optimum solution of the problem while meeting the strict functional limits for the technical and economic indicators of the driverless vehicle control system under development is effective.

  7. Karyotype stability and unbiased fractionation in the paleo-allotetraploid Cucurbita genomes

    OpenAIRE

    Sun, Honghe; Wu, Shan; Zhang, Guoyu; Jiao, Chen; Guo, Shaogui; Ren, Yi; Zhang, Jie; Zhang, Haiying; Gong, Guoyi; Jia, Zhangcai; Zhang, Fan; Tian, Jiaxing; Lucas, William; Doyle, Jeff; Li, Haizhen

    2017-01-01

    The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Interspecific hybrids between C. maxima and C. moschata are widely used as rootstocks for other cucurbit crops. We report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetraploidization event in Cucurbita. We are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber and watermelon in...

  8. Studies on the effects of persistent RNA priming on DNA replication and genomic stability

    OpenAIRE

    Stuckey, Ruth

    2014-01-01

    [EN]: DNA replication and transcription take place on the same DNA template, and the correct interplay between these processes ensures faithful genome duplication. DNA replication must be highly coordinated with other cell cycle events, such as segregation of fully replicated DNA in order to maintain genomic integrity. Transcription generates RNA:DNA hybrids, transient intermediate structures that are degraded by the ribonuclease H (RNaseH) class of enzymes. RNA:DNA hybrids can form R-loops, ...

  9. Exponential stability for formation control systems with generalized controllers: A unified approach

    NARCIS (Netherlands)

    Sun, Zhiyong; Mou, Shaoshuai; Anderson, Brian D.O.; Cao, Ming

    2016-01-01

    This paper discusses generalized controllers for distance-based rigid formation shape stabilization and aims to provide a unified approach for the convergence analysis. We consider two types of formation control systems according to different characterizations of target formations: minimally rigid

  10. 76 FR 49532 - Federal Motor Vehicle Safety Standards; Electronic Stability Control; Technical Report on the...

    Science.gov (United States)

    2011-08-10

    ...-0112] Federal Motor Vehicle Safety Standards; Electronic Stability Control; Technical Report on the Effectiveness of Electronic Stability Control Systems for Cars and LTVs AGENCY: National Highway Traffic Safety..., Electronic Stability Control Systems. The report's title is: Crash Prevention Effectiveness in Light-Vehicle...

  11. A comparative study of stabilizing control of a planer electromagnetic levitation using PID and LQR controllers

    Directory of Open Access Journals (Sweden)

    Mundher H.A. Yaseen

    Full Text Available Magnetic levitation is a technique to suspend an object without any mechanical support. The main objective of this study is to demonstrate stabilized closed loop control of 1-DOF Maglev experimentally using real-time control simulink feature of (SIMLAB microcontroller. Proportional Integral Derivative (PID and Linear Quadratic Regulator (LQR controllers are employed to examine the stability performance of the Maglev control system under effect of unbalanced change of load and wave signal on Maglev plane. The effect of unbalanced change of applied load on single point, line and plane are presented. Furthermore, in order to study the effect of sudden change in input signal, the input of wave signal has been applied on all points of the prototype maglev plate simultaneously. The results of pulse width modulation (PWM reveal that the control system using LQR controller provides faster response to adjust the levitated plane comparing to PID controller. Moreover, the air gap distance that controlled using PID controller is rather stable with little oscillation. Meanwhile, LQR controller provided more stability and homogeneous response. Keywords: Magnetic levitation (Maglev, Linear Quadratic Regulator (LQR, PID controller, SIMLAB Platform

  12. Information spread in networks: Games, optimal control, and stabilization

    Science.gov (United States)

    Khanafer, Ali

    This thesis focuses on designing efficient mechanisms for controlling information spread in networks. We consider two models for information spread. The first one is the well-known distributed averaging dynamics. The second model is a nonlinear one that describes virus spread in computer and biological networks. We seek to design optimal, robust, and stabilizing controllers under practical constraints. For distributed averaging networks, we study the interaction between a network designer and an adversary. We consider two types of attacks on the network. In Attack-I, the adversary strategically disconnects a set of links to prevent the nodes from reaching consensus. Meanwhile, the network designer assists the nodes in reaching consensus by changing the weights of a limited number of links in the network. We formulate two problems to describe this competition where the order in which the players act is reversed in the two problems. Although the canonical equations provided by the Pontryagin's Maximum Principle (MP) seem to be intractable, we provide an alternative characterization for the optimal strategies that makes connection to potential theory. Further, we provide a sufficient condition for the existence of a saddle-point equilibrium (SPE) for the underlying zero-sum game. In Attack-II, the designer and the adversary are both capable of altering the measurements of all nodes in the network by injecting global signals. We impose two constraints on both players: a power constraint and an energy constraint. We assume that the available energy to each player is not sufficient to operate at maximum power throughout the horizon of the game. We show the existence of an SPE and derive the optimal strategies in closed form for this attack scenario. As an alternative to the "network designer vs. adversary" framework, we investigate the possibility of stabilizing unknown network diffusion processes using a distributed mechanism, where the uncertainty is due to an attack

  13. A comparative study of stabilizing control of a planer electromagnetic levitation using PID and LQR controllers

    Science.gov (United States)

    Yaseen, Mundher H. A.

    Magnetic levitation is a technique to suspend an object without any mechanical support. The main objective of this study is to demonstrate stabilized closed loop control of 1-DOF Maglev experimentally using real-time control simulink feature of (SIMLAB) microcontroller. Proportional Integral Derivative (PID) and Linear Quadratic Regulator (LQR) controllers are employed to examine the stability performance of the Maglev control system under effect of unbalanced change of load and wave signal on Maglev plane. The effect of unbalanced change of applied load on single point, line and plane are presented. Furthermore, in order to study the effect of sudden change in input signal, the input of wave signal has been applied on all points of the prototype maglev plate simultaneously. The results of pulse width modulation (PWM) reveal that the control system using LQR controller provides faster response to adjust the levitated plane comparing to PID controller. Moreover, the air gap distance that controlled using PID controller is rather stable with little oscillation. Meanwhile, LQR controller provided more stability and homogeneous response.

  14. Patient-controlled encrypted genomic data: an approach to advance clinical genomics

    Directory of Open Access Journals (Sweden)

    Trakadis Yannis J

    2012-07-01

    Full Text Available Abstract Background The revolution in DNA sequencing technologies over the past decade has made it feasible to sequence an individual’s whole genome at a relatively low cost. The potential value of the information generated by genomic technologies for medicine and society is enormous. However, in order for exome sequencing, and eventually whole genome sequencing, to be implemented clinically, a number of major challenges need to be overcome. For instance, obtaining meaningful informed-consent, managing incidental findings and the great volume of data generated (including multiple findings with uncertain clinical significance, re-interpreting the genomic data and providing additional counselling to patients as genetic knowledge evolves are issues that need to be addressed. It appears that medical genetics is shifting from the present “phenotype-first” medical model to a “data-first” model which leads to multiple complexities. Discussion This manuscript discusses the different challenges associated with integrating genomic technologies into clinical practice and describes a “phenotype-first” approach, namely, “Individualized Mutation-weighed Phenotype Search”, and its benefits. The proposed approach allows for a more efficient prioritization of the genes to be tested in a clinical lab based on both the patient’s phenotype and his/her entire genomic data. It simplifies “informed-consent” for clinical use of genomic technologies and helps to protect the patient’s autonomy and privacy. Overall, this approach could potentially render widespread use of genomic technologies, in the immediate future, practical, ethical and clinically useful. Summary The “Individualized Mutation-weighed Phenotype Search” approach allows for an incremental integration of genomic technologies into clinical practice. It ensures that we do not over-medicalize genomic data but, rather, continue our current medical model which is based on serving

  15. On a program manifold's stability of one contour automatic control systems

    Science.gov (United States)

    Zumatov, S. S.

    2017-12-01

    Methodology of analysis of stability is expounded to the one contour systems automatic control feedback in the presence of non-linearities. The methodology is based on the use of the simplest mathematical models of the nonlinear controllable systems. Stability of program manifolds of one contour automatic control systems is investigated. The sufficient conditions of program manifold's absolute stability of one contour automatic control systems are obtained. The Hurwitz's angle of absolute stability was determined. The sufficient conditions of program manifold's absolute stability of control systems by the course of plane in the mode of autopilot are obtained by means Lyapunov's second method.

  16. Stability and linearity control of spectrometric channels of the Cherenkov counters using controllable units

    International Nuclear Information System (INIS)

    Kollar, D.; Kollarova, L.; Khorvat, P.

    1976-01-01

    A system is elaborated to control stability and linearity of the Cherenkov counter spectrometric channels in an experiment on a magnetic monopole search. Linearity of a light characteristic of a photoelectric multiplier is checked with the help of the calibrated light-strikings of light emitting diodes with flare intensity adjusted by controlling generator voltage across the mercury body. A program algorithm is presented for checking stability and linearity of the Cherenkov counter spectrometric channels which helps to consider the fatigue effects of the photoelectric multiplier resulting from the considerable loads

  17. Distributed stability control using intelligent voltage-margin relay

    Energy Technology Data Exchange (ETDEWEB)

    Wiszniewski, A.; Rebizant, W. [Wroclaw Univ. of Technology (Poland); Klimek, A. [Powertech Labs Inc., Surrey, BC (Canada)

    2010-07-01

    This paper presented an intelligent relay that operates if the load to source impedance ratio decreases to a level that is dangerously close to the stability limit, which leads to power system blackouts. The intelligent voltage-margin/difference relay installed at receiving substations automatically initiates action if the voltage stability margin drops to a dangerously low level. The relay decides if the tap changing devices are to be blocked and if under-voltage load shedding should be initiated, thereby mitigating an evolving instability. The intelligent relay has two levels of operation. At the first stage, which corresponds to the higher load to source impedance ratio, the relay initiates blocking of the tap changer. At the second stage, corresponding to the lower source to load impedance ratio, load shedding is initiated. The relay operates when the load to source impedance ratio reaches a certain predetermined level, but it does not depend either on the level of the source voltage or on the difference of source and load impedance phase angles. The algorithm for the relay is relatively simple and uses only locally available signals. Consequently, the transformer is well controlled to eliminate the cases of voltage instability. 6 refs., 7 figs.

  18. Design and Stability of an On-Orbit Attitude Control System Using Reaction Control Thrusters

    Science.gov (United States)

    Hall, Robert A.; Hough, Steven; Orphee, Carolina; Clements, Keith

    2016-01-01

    Basic principles for the design and stability of a spacecraft on-orbit attitude control system employing on-off Reaction Control System (RCS) thrusters are presented. Both vehicle dynamics and the control system actuators are inherently nonlinear, hence traditional linear control system design approaches are not directly applicable. This paper has two main aspects: It summarizes key RCS design principles from earlier NASA vehicles, notably the Space Shuttle and Space Station programs, and introduces advances in the linear modelling and analyses of a phase plane control system derived in the initial development of the NASA's next upper stage vehicle, the Exploration Upper Stage (EUS). Topics include thruster hardware specifications, phase plane design and stability, jet selection approaches, filter design metrics, and RCS rotational maneuver logic.

  19. White spot syndrome virus (WSSV) genome stability maintained over six passages through three different penaeid shrimp species.

    Science.gov (United States)

    Sindhupriya, M; Saravanan, P; Otta, S K; Amarnath, C Bala; Arulraj, R; Bhuvaneswari, T; Praveena, P Ezhil; Jithendran, K P; Ponniah, A G

    2014-08-21

    White spot syndrome virus (WSSV) replicates rapidly, can be extremely pathogenic and is a common cause of mass mortality in cultured shrimp. Variable number tandem repeat (VNTR) sequences present in the open reading frame (ORF)94, ORF125 and ORF75 regions of the WSSV genome have been used widely as genetic markers in epidemiological studies. However, reports that VNTRs might evolve rapidly following even a single transmission through penaeid shrimp or other crustacean hosts have created confusion as to how VNTR data is interpreted. To examine VNTR stability again, 2 WSSV strains (PmTN4RU and LvAP11RU) with differing ORF94 tandem repeat numbers and slight differences in apparent virulence were passaged sequentially 6 times through black tiger shrimp Penaeus monodon, Indian white shrimp Feneropenaeus indicus or Pacific white leg shrimp Litopenaeus vannamei. PCR analyses to genotype the ORF94, ORF125 and ORF75 VNTRs did not identify any differences from either of the 2 parental WSSV strains after multiple passages through any of the shrimp species. These data were confirmed by sequence analysis and indicate that the stability of the genome regions containing these VNTRs is quite high at least for the WSSV strains, hosts and number of passages examined and that the VNTR sequences thus represent useful genetic markers for studying WSSV epidemiology.

  20. The Arabidopsis thaliana Homolog of the Helicase RTEL1 Plays Multiple Roles in Preserving Genome Stability[C][W

    Science.gov (United States)

    Recker, Julia; Knoll, Alexander; Puchta, Holger

    2014-01-01

    In humans, mutations in the DNA helicase Regulator of Telomere Elongation Helicase1 (RTEL1) lead to Hoyeraal-Hreidarsson syndrome, a severe, multisystem disorder. Here, we demonstrate that the RTEL1 homolog in Arabidopsis thaliana plays multiple roles in preserving genome stability. RTEL1 suppresses homologous recombination in a pathway parallel to that of the DNA translocase FANCM. Cytological analyses of root meristems indicate that RTEL1 is involved in processing DNA replication intermediates independently from FANCM and the nuclease MUS81. Moreover, RTEL1 is involved in interstrand and intrastrand DNA cross-link repair independently from FANCM and (in intrastrand cross-link repair) parallel to MUS81. RTEL1 contributes to telomere homeostasis; the concurrent loss of RTEL1 and the telomerase TERT leads to rapid, severe telomere shortening, which occurs much more rapidly than it does in the single-mutant line tert, resulting in developmental arrest after four generations. The double mutant rtel1-1 recq4A-4 exhibits massive growth defects, indicating that this RecQ family helicase, which is also involved in the suppression of homologous recombination and the repair of DNA lesions, can partially replace RTEL1 in the processing of DNA intermediates. The requirement for RTEL1 in multiple pathways to preserve genome stability in plants can be explained by its putative role in the destabilization of DNA loop structures, such as D-loops and T-loops. PMID:25516598

  1. Stabilizing photoassociated Cs2 molecules by optimal control

    International Nuclear Information System (INIS)

    Zhang Wei; Xie Ting; Huang Yin; Wang Gao-Ren; Cong Shu-Lin

    2013-01-01

    We demonstrate theoretically that photoassociated molecules can be stabilized to deeply bound states. This process is achieved by transferring the population from the outer well to the inner well using the optimal control theory, the Cs 2 molecule is taken as an example. Numerical calculations show that weakly bound molecules formed in the outer well by a pump pulse can be compressed to the inner well via a vibrational level of the ground electronic state as an intermediary by an additionally optimized laser pulse. The positively chirped pulse can enhance the population of the target state. With a transform-limited dump pulse, nearly all the photoassociated molecules in the inner well of the excited electronic state can be transferred to the deeply vibrational level of the ground electronic state. (atomic and molecular physics)

  2. Stability investigation of an airfoil section with active flap control

    DEFF Research Database (Denmark)

    Bergami, Leonardo; Gaunaa, Mac

    2010-01-01

    function approximation. Stability of the full aeroservoelastic system is determined through eigenvalue analysis by state-space formulation of the indicial approximation. Validation is carried out against an implementation of the recursive method by Theodorsen and Garrick for flexure-torsion-aileron flutter...... for fatigue load alleviation. The structural model of the 2-D airfoil section contains three degrees of freedom: heave translation, pitch rotation and flap deflection. A potential flow model provides the aerodynamic forces and their distribution. The unsteady aerodynamics are described using an indicial...... on measurements of either heave displacement, local angle of attack or aerodynamic pressure difference measured over the airfoil. The purpose of the controlled deformable flap is to reduce fluctuations in the aerodynamic forces on the airfoil, which, according to recent studies, have a significant potential...

  3. Stabilizing photoassociated Cs2 molecules by optimal control

    Science.gov (United States)

    Zhang, Wei; Xie, Ting; Huang, Yin; Wang, Gao-Ren; Cong, Shu-Lin

    2013-01-01

    We demonstrate theoretically that photoassociated molecules can be stabilized to deeply bound states. This process is achieved by transferring the population from the outer well to the inner well using the optimal control theory, the Cs2 molecule is taken as an example. Numerical calculations show that weakly bound molecules formed in the outer well by a pump pulse can be compressed to the inner well via a vibrational level of the ground electronic state as an intermediary by an additionally optimized laser pulse. The positively chirped pulse can enhance the population of the target state. With a transform-limited dump pulse, nearly all the photoassociated molecules in the inner well of the excited electronic state can be transferred to the deeply vibrational level of the ground electronic state.

  4. Orbit stability and feedback control in synchrotron radiation rings

    International Nuclear Information System (INIS)

    Yu, L.H.

    1989-01-01

    Stability of the electron orbit is essential for the utilization of a low emittance storage ring as a high brightness radiation source. We discuss the development of the measurement and feedback control of the closed orbit, with emphasis on the activities as the National Synchrotron Light Source of BNL. We discuss the performance of the beam position detectors in use and under development: the PUE rf detector, split ion chamber detector, photo-emission detector, solid state detector, and the graphite detector. Depending on the specific experiments, different beamlines require different tolerances on the orbit motion. Corresponding to these different requirements, we discuss two approaches to closed orbit feedback: the global and local feedback systems. Then we describe a new scheme for the real time global feedback by implementing a feedback system based upon a harmonic analysis of both the orbit movements and the correction magnetic fields. 14 refs., 6 figs., 2 tabs

  5. High level model predictive control for plug-and-play process control with stability guaranty

    DEFF Research Database (Denmark)

    Michelsen, Axel Gottlieb; Stoustrup, Jakob

    2010-01-01

    In this paper a method for designing a stabilizing high level model predictive controller for a hierarchical plug- and-play process is presented. This is achieved by abstracting the lower layers of the controller structure as low order models with uncertainty and by using a robust model predictive...... controller for generating the references for these. A simulation example, in which the actuators in a process control system are changed, is reported to show the potential of this approach for plug and play process control....

  6. Vehicle rollover risk and electronic stability control systems.

    Science.gov (United States)

    MacLennan, P A; Marshall, T; Griffin, R; Purcell, M; McGwin, G; Rue, L W

    2008-06-01

    Electronic stability control (ESC) systems were developed to reduce motor vehicle collisions (MVCs) caused by loss of control. Introduced in Europe in 1995 and in the USA in 1996, ESC is designed to improve vehicle lateral stability by electronically detecting and automatically assisting drivers in unfavorable situations. To examine the relationship between vehicle rollover risk and presence of ESC using a large national database of MVCs. A retrospective cohort study for the period 1995 through 2006 was carried out using data obtained from the National Automotive Sampling System General Estimates System. All passenger cars and sport utility vehicles (SUVs)/vans of model year 1996 and later were eligible. Vehicle ESC (unavailable, optional, standard) was determined on the basis of make, model, and model year. Risk ratios (RRs) and 95% CIs were calculated to compare rollover risk by vehicle ESC group. For all crashes, vehicles equipped with standard ESC had decreased risk of rollover (RR = 0.62, 95% CI 0.50 to 0.77) compared with vehicles with ESC unavailable. The association was consistent for single-vehicle MVCs (RR = 0.61, 95% CI 0.46 to 0.82); passenger cars had decreased rollover risk (RR = 0.77, 95% CI 0.52 to 1.12), but SUVs/vans had a more dramatically decreased risk (RR = 0.40, 95% CI 0.26 to 0.61). This study supports previous results showing ESC to be effective in reducing the risk of rollover. ESC is more effective in SUVs/vans for rollovers related to single-vehicle MVCs.

  7. Landslide Geohazard Monitoring, Early Warning and Stabilization Control Methods

    Science.gov (United States)

    Bednarczyk, Zbigniew

    2014-03-01

    This paper is a presentation of landslide monitoring, early warning and remediation methods recommended for the Polish Carpathians. Instrumentation included standard and automatic on-line measurements with the real-time transfer of data to an Internet web server. The research was funded through EU Innovative Economy Programme and also by the SOPO Landslide Counteraction Project. The landslides investigated were characterized by relatively low rates of the displacements. These ranged from a few millimetres to several centimetres per year. Colluviums of clayey flysch deposits were of a soil-rock type with a very high plasticity and moisture content. The instrumentation consisted of 23 standard inclinometers set to depths of 5-21 m. The starting point of monitoring measurements was in January 2006. These were performed every 1-2 months over the period of 8 years. The measurements taken detected displacements from several millimetres to 40 cm set at a depth of 1-17 m. The modern, on-line monitoring and early warning system was installed in May 2010. The system is the first of its kind in Poland and only one of several such real-time systems in the world. The installation was working with the Local Road Authority in Gorlice. It contained three automatic field stations for investigation of landslide parameters to depths of 12-16 m and weather station. In-place tilt transducers and innovative 3D continuous inclinometer systems with sensors located every 0.5 m were used. It has the possibility of measuring a much greater range of movements compared to standard systems. The conventional and real-time data obtained provided a better recognition of the triggering parameters and the control of geohazard stabilizations. The monitoring methods chosen supplemented by numerical modelling could lead to more reliable forecasting of such landslides and could thus provide better control and landslide remediation possibilities also to stabilization works which prevent landslides.

  8. Landslide Geohazard Monitoring, Early Warning and Stabilization Control Methods

    Directory of Open Access Journals (Sweden)

    Bednarczyk Zbigniew

    2014-03-01

    Full Text Available This paper is a presentation of landslide monitoring, early warning and remediation methods recommended for the Polish Carpathians. Instrumentation included standard and automatic on-line measurements with the real-time transfer of data to an Internet web server. The research was funded through EU Innovative Economy Programme and also by the SOPO Landslide Counteraction Project. The landslides investigated were characterized by relatively low rates of the displacements. These ranged from a few millimetres to several centimetres per year. Colluviums of clayey flysch deposits were of a soil-rock type with a very high plasticity and moisture content. The instrumentation consisted of 23 standard inclinometers set to depths of 5-21 m. The starting point of monitoring measurements was in January 2006. These were performed every 1-2 months over the period of 8 years. The measurements taken detected displacements from several millimetres to 40 cm set at a depth of 1-17 m. The modern, on-line monitoring and early warning system was installed in May 2010. The system is the first of its kind in Poland and only one of several such real-time systems in the world. The installation was working with the Local Road Authority in Gorlice. It contained three automatic field stations for investigation of landslide parameters to depths of 12-16 m and weather station. In-place tilt transducers and innovative 3D continuous inclinometer systems with sensors located every 0.5 m were used. It has the possibility of measuring a much greater range of movements compared to standard systems. The conventional and real-time data obtained provided a better recognition of the triggering parameters and the control of geohazard stabilizations. The monitoring methods chosen supplemented by numerical modelling could lead to more reliable forecasting of such landslides and could thus provide better control and landslide remediation possibilities also to stabilization works which

  9. The genomic diversity and stability of field strains of Suid herpesvirus 1 (Aujeszky's disease virus)

    DEFF Research Database (Denmark)

    Christensen, Laurids Siig; Sørensen, K. J.

    1991-01-01

    The genomic diversity among isolates of suid herpesvirus 1 (SHV-1) collected in the same herd and among clones from the same isolate was studied by restriction fragment pattern (RFP) analysis using BamHI. Tentatively defining a field strain as a transmissible entity, it was concluded that strains...

  10. Stabilization of amorphous calcium carbonate by controlling its particle size

    NARCIS (Netherlands)

    Nudelman, F.; Sonmezler, E.; Bomans, P.H.H.; With, de G.; Sommerdijk, N.A.J.M.

    2010-01-01

    Amorphous calcium carbonate (ACC) nanoparticles of different size are prepared using a flow system. Post-synthesis stabilization with a layer of poly[(a,ß)-DL-aspartic acid] leads to stabilization of the ACC, but only for particles

  11. BRIT1/MCPH1 is essential for mitotic and meiotic recombination DNA repair and maintaining genomic stability in mice.

    Directory of Open Access Journals (Sweden)

    Yulong Liang

    2010-01-01

    Full Text Available BRIT1 protein (also known as MCPH1 contains 3 BRCT domains which are conserved in BRCA1, BRCA2, and other important molecules involved in DNA damage signaling, DNA repair, and tumor suppression. BRIT1 mutations or aberrant expression are found in primary microcephaly patients as well as in cancer patients. Recent in vitro studies suggest that BRIT1/MCPH1 functions as a novel key regulator in the DNA damage response pathways. To investigate its physiological role and dissect the underlying mechanisms, we generated BRIT1(-/- mice and identified its essential roles in mitotic and meiotic recombination DNA repair and in maintaining genomic stability. Both BRIT1(-/- mice and mouse embryonic fibroblasts (MEFs were hypersensitive to gamma-irradiation. BRIT1(-/- MEFs and T lymphocytes exhibited severe chromatid breaks and reduced RAD51 foci formation after irradiation. Notably, BRIT1(-/- mice were infertile and meiotic homologous recombination was impaired. BRIT1-deficient spermatocytes exhibited a failure of chromosomal synapsis, and meiosis was arrested at late zygotene of prophase I accompanied by apoptosis. In mutant spermatocytes, DNA double-strand breaks (DSBs were formed, but localization of RAD51 or BRCA2 to meiotic chromosomes was severely impaired. In addition, we found that BRIT1 could bind to RAD51/BRCA2 complexes and that, in the absence of BRIT1, recruitment of RAD51 and BRCA2 to chromatin was reduced while their protein levels were not altered, indicating that BRIT1 is involved in mediating recruitment of RAD51/BRCA2 to the damage site. Collectively, our BRIT1-null mouse model demonstrates that BRIT1 is essential for maintaining genomic stability in vivo to protect the hosts from both programmed and irradiation-induced DNA damages, and its depletion causes a failure in both mitotic and meiotic recombination DNA repair via impairing RAD51/BRCA2's function and as a result leads to infertility and genomic instability in mice.

  12. European Chlamydia abortus livestock isolate genomes reveal unusual stability and limited diversity, reflected in geographical signatures.

    Science.gov (United States)

    Seth-Smith, H M B; Busó, Leonor Sánchez; Livingstone, M; Sait, M; Harris, S R; Aitchison, K D; Vretou, Evangelia; Siarkou, V I; Laroucau, K; Sachse, K; Longbottom, D; Thomson, N R

    2017-05-04

    Chlamydia abortus (formerly Chlamydophila abortus) is an economically important livestock pathogen, causing ovine enzootic abortion (OEA), and can also cause zoonotic infections in humans affecting pregnancy outcome. Large-scale genomic studies on other chlamydial species are giving insights into the biology of these organisms but have not yet been performed on C. abortus. Our aim was to investigate a broad collection of European isolates of C. abortus, using next generation sequencing methods, looking at diversity, geographic distribution and genome dynamics. Whole genome sequencing was performed on our collection of 57 C. abortus isolates originating primarily from the UK, Germany, France and Greece, but also from Tunisia, Namibia and the USA. Phylogenetic analysis of a total of 64 genomes shows a deep structural division within the C. abortus species with a major clade displaying limited diversity, in addition to a branch carrying two more distantly related Greek isolates, LLG and POS. Within the major clade, seven further phylogenetic groups can be identified, demonstrating geographical associations. The number of variable nucleotide positions across the sampled isolates is significantly lower than those published for C. trachomatis and C. psittaci. No recombination was identified within C. abortus, and no plasmid was found. Analysis of pseudogenes showed lineage specific loss of some functions, notably with several Pmp and TMH/Inc proteins predicted to be inactivated in many of the isolates studied. The diversity within C. abortus appears to be much lower compared to other species within the genus. There are strong geographical signatures within the phylogeny, indicating clonal expansion within areas of limited livestock transport. No recombination has been identified within this species, showing that different species of Chlamydia may demonstrate different evolutionary dynamics, and that the genome of C. abortus is highly stable.

  13. Effectiveness of electronic stability control on single-vehicle accidents.

    Science.gov (United States)

    Lyckegaard, Allan; Hels, Tove; Bernhoft, Inger Marie

    2015-01-01

    This study aims at evaluating the effectiveness of electronic stability control (ESC) on single-vehicle injury accidents while controlling for a number of confounders influencing the accident risk. Using police-registered injury accidents from 2004 to 2011 in Denmark with cars manufactured in the period 1998 to 2011 and the principle of induced exposure, 2 measures of the effectiveness of ESC were calculated: The crude odds ratio and the adjusted odds ratio, the latter by means of logistic regression. The logistic regression controlled for a number of confounding factors, of which the following were significant. For the driver: Age, gender, driving experience, valid driving license, and seat belt use. For the vehicle: Year of registration, weight, and ESC. For the accident surroundings: Visibility, light, and location. Finally, for the road: Speed limit, surface, and section characteristics. The present study calculated the crude odds ratio for ESC-equipped cars of getting in a single-vehicle injury accident as 0.40 (95% confidence interval [CI], 0.34-0.47) and the adjusted odds ratio as 0.69 (95% CI, 0.54-0.88). No difference was found in the effectiveness of ESC across the injury severity categories (slight, severe, and fatal). In line with previous results, this study concludes that ESC reduces the risk for single-vehicle injury accidents by 31% when controlling for various confounding factors related to the driver, the car, and the accident surroundings. Furthermore, it is concluded that it is important to control for human factors (at a minimum age and gender) in analyses where evaluations of this type are performed.

  14. Quality control and conduct of genome-wide association meta-analyses

    DEFF Research Database (Denmark)

    Winkler, Thomas W; Day, Felix R; Croteau-Chonka, Damien C

    2014-01-01

    Rigorous organization and quality control (QC) are necessary to facilitate successful genome-wide association meta-analyses (GWAMAs) of statistics aggregated across multiple genome-wide association studies. This protocol provides guidelines for (i) organizational aspects of GWAMAs, and for (ii) QC...

  15. Genome sequences of three strains of Aspergillus flavus for the biological control of Aflatoxin

    Science.gov (United States)

    The genomes of three strains of Aspergillus flavus with demonstrated utility for the biological control of aflatoxin were sequenced. These sequences were assembled with MIRA and annotated with Augustus using A. flavus strain 3357 (NCBI EQ963472) as a reference. Each strain had a genome of 36.3 to ...

  16. Fixed-Time Stability Analysis of Permanent Magnet Synchronous Motors with Novel Adaptive Control

    Directory of Open Access Journals (Sweden)

    Maoxing Liu

    2017-01-01

    Full Text Available We firstly investigate the fixed-time stability analysis of uncertain permanent magnet synchronous motors with novel control. Compared with finite-time stability where the convergence rate relies on the initial permanent magnet synchronous motors state, the settling time of fixed-time stability can be adjusted to desired values regardless of initial conditions. Novel adaptive stability control strategy for the permanent magnet synchronous motors is proposed, with which we can stabilize permanent magnet synchronous motors within fixed time based on the Lyapunov stability theory. Finally, some simulation and comparison results are given to illustrate the validity of the theoretical results.

  17. Electric Vehicle Longitudinal Stability Control Based on a New Multimachine Nonlinear Model Predictive Direct Torque Control

    Directory of Open Access Journals (Sweden)

    M’hamed Sekour

    2017-01-01

    Full Text Available In order to improve the driving performance and the stability of electric vehicles (EVs, a new multimachine robust control, which realizes the acceleration slip regulation (ASR and antilock braking system (ABS functions, based on nonlinear model predictive (NMP direct torque control (DTC, is proposed for four permanent magnet synchronous in-wheel motors. The in-wheel motor provides more possibilities of wheel control. One of its advantages is that it has low response time and almost instantaneous torque generation. Moreover, it can be independently controlled, enhancing the limits of vehicular control. For an EV equipped with four in-wheel electric motors, an advanced control may be envisaged. Taking advantage of the fast and accurate torque of in-wheel electric motors which is directly transmitted to the wheels, a new approach for longitudinal control realized by ASR and ABS is presented in this paper. In order to achieve a high-performance torque control for EVs, the NMP-DTC strategy is proposed. It uses the fuzzy logic control technique that determines online the accurate values of the weighting factors and generates the optimal switching states that optimize the EV drives’ decision. The simulation results built in Matlab/Simulink indicate that the EV can achieve high-performance vehicle longitudinal stability control.

  18. Evaluation of electronic stability control effectiveness in Australasia.

    Science.gov (United States)

    Scully, Jim; Newstead, Stuart

    2008-11-01

    Electronic stability control (ESC) is an in-vehicle technology aimed at improving primary safety by assisting the driver in avoiding loss of control of the vehicle. The aim of this study was to use available crash data from Australia and New Zealand to evaluate the effectiveness of ESC in reducing crash risk and to establish whether benefits estimated from overseas studies have translated to the Australian and New Zealand environments. The sample analysed included 7699 crashed vehicles fitted with ESC which comprised of 90 different models. Poisson regression was used to test whether the differences in the observed and expected crash counts for ESC fitted vehicles were significant, with exposure being induced from counts of rear end impacts. It was found that ESC reduced the risk of single vehicle crashes in which the driver was injured by 68% for 4WDs compared with 27% for passenger cars. The effect of ESC on multiple vehicle crashes in Australia and New Zealand was not clear. The long-term benefits of fitting ESC to all vehicles in Australia were also investigated based on the estimated single vehicle crash reductions.

  19. Car drivers' perceptions of electronic stability control (ESC) systems.

    Science.gov (United States)

    Vadeby, Anna; Wiklund, Mats; Forward, Sonja

    2011-05-01

    As a way to reduce the number of car crashes different in-car safety devices are being introduced. In this paper one such application is being investigated, namely the electronic stability control system (ESC). The study used a survey method, including 2000 private car drivers (1000 driving a car with ESC and 1000 driving a car without ESC). The main objective was to investigate the effect of ESC on driver behaviour. Results show that drivers report that they drive even more carelessly when they believe that they have ESC, than when they do not. Men are more risk prone than women and young drivers more than older drivers. Using the theory of planned behaviour the results show that attitude, subjective norm and perceived control explain between 62% and 67% of driver's variation of intentions to take risks. When descriptive norm was added to the model a small but statistically significant increase was found. The study also shows that more than 35% erroneously believe that their car is equipped with an ESC system. These findings may suggest that driver behaviour could reduce the positive effect ESC has on accidents. It also shows that drivers who purchase a new car are not well informed about what kind of safety devices the car is equipped with. These findings highlight the need for more targeted information to drivers. Copyright © 2010 Elsevier Ltd. All rights reserved.

  20. Design and Implementation of a Randomized Controlled Trial of Genomic Counseling for Patients with Chronic Disease

    Directory of Open Access Journals (Sweden)

    Kevin Sweet

    2014-01-01

    Full Text Available We describe the development and implementation of a randomized controlled trial to investigate the impact of genomic counseling on a cohort of patients with heart failure (HF or hypertension (HTN, managed at a large academic medical center, the Ohio State University Wexner Medical Center (OSUWMC. Our study is built upon the existing Coriell Personalized Medicine Collaborative (CPMC®. OSUWMC patient participants with chronic disease (CD receive eight actionable complex disease and one pharmacogenomic test report through the CPMC® web portal. Participants are randomized to either the in-person post-test genomic counseling—active arm, versus web-based only return of results—control arm. Study-specific surveys measure: (1 change in risk perception; (2 knowledge retention; (3 perceived personal control; (4 health behavior change; and, for the active arm (5, overall satisfaction with genomic counseling. This ongoing partnership has spurred creation of both infrastructure and procedures necessary for the implementation of genomics and genomic counseling in clinical care and clinical research. This included creation of a comprehensive informed consent document and processes for prospective return of actionable results for multiple complex diseases and pharmacogenomics (PGx through a web portal, and integration of genomic data files and clinical decision support into an EPIC-based electronic medical record. We present this partnership, the infrastructure, genomic counseling approach, and the challenges that arose in the design and conduct of this ongoing trial to inform subsequent collaborative efforts and best genomic counseling practices.

  1. Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase.

    Science.gov (United States)

    Vettone, Antonella; Perugino, Giuseppe; Rossi, Mosè; Valenti, Anna; Ciaramella, Maria

    2014-09-01

    Repair and defence of genome integrity from endogenous and environmental hazard is a primary need for all organisms. Natural selection has driven the evolution of multiple cell pathways to deal with different DNA damaging agents. Failure of such processes can hamper cell functions and induce inheritable mutations, which in humans may cause cancerogenicity or certain genetic syndromes, and ultimately cell death. A special case is that of hyperthermophilic bacteria and archaea, flourishing at temperatures higher than 80 °C, conditions that favor genome instability and thus call for specific, highly efficient or peculiar mechanisms to keep their genome intact and functional. Over the last few years, numerous studies have been performed on the activity, function, regulation, physical and functional interaction of enzymes and proteins from hyperthermophilic microorganisms that are able to bind, repair, bypass damaged DNA, or modify its structure or conformation. The present review is focused on two enzymes that act on DNA catalyzing unique reactions: reverse gyrase and DNA alkyltransferase. Although both enzymes belong to evolutionary highly conserved protein families present in organisms of the three domains (Eucarya, Bacteria and Archaea), recently characterized members from hyperthermophilic archaea show both common and peculiar features.

  2. A Network of Multi-Tasking Proteins at the DNA Replication Fork Preserves Genome Stability.

    Directory of Open Access Journals (Sweden)

    2005-12-01

    Full Text Available To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately 4,700 viable yeast deletion mutants and determined the fitness of the double mutants. Fifty-six DNA2-interacting genes were identified. Clustering analysis of genomic synthetic lethality profiles of each of 43 of the DNA2-interacting genes defines a network (consisting of 322 genes and 876 interactions whose topology provides clues as to how replication proteins coordinate regulation and repair to protect genome integrity. The results also shed new light on the functions of the query gene DNA2, which, despite many years of study, remain controversial, especially its proposed role in Okazaki fragment processing and the nature of its in vivo substrates. Because of the multifunctional nature of virtually all proteins at the replication fork, the meaning of any single genetic interaction is inherently ambiguous. The multiplexing nature of the current studies, however, combined with follow-up supporting experiments, reveals most if not all of the unique pathways requiring Dna2p. These include not only Okazaki fragment processing and DNA repair but also chromatin dynamics.

  3. Stability of Dry Probiotic Bacteria in Relation to the Cellular Membrane and Genomic DNA

    DEFF Research Database (Denmark)

    Hansen, Marie-Louise Rittermann W

    powders to infant formula and functional foods, e.g. cereal and chocolate bars. Part of production of the probiotic product often includes freeze-drying of the probiotic bacteria. Drying is necessary in the preparation of dry powders, and to increase shelf life of the product at ambient temperatures....... Freeze-drying and storage of probiotic bacteria can, however, also have a negative effect on cell stability. It is important to understand the processes, which can lead to loss of cell stability during dry storage in order to develop more stable probiotic products. The purpose of the present PhD thesis...

  4. Stability and control of wind farms in power systems

    Energy Technology Data Exchange (ETDEWEB)

    Jauch, C.

    2006-10-15

    The Ph.D. project 'Stability and Control of Wind Farms in Power Systems' deals with some selected problems related to wind power in power systems. With increasing wind power penetration, wind turbines substitute the power production of conventional power plants. Therefore, wind turbines also have to take over the power system stabilisation and control tasks, that were traditionally carried out by conventional power plants. Out of the many aspects related to this problem, this project focuses on transient fault ride-through and power system stabilisation. The selection of turbine types considered in this project is limited to active-stall turbines and variable speed, variable pitch turbines with gearboxes and full-scale converter-connected synchronous generators. As a basis for the project, a study into the state of the art is conducted at the beginning of the project. Grid connection requirements that were in force, or published as drafts, at the time, and scientific literature related to the topic, are studied. The project is based on simulations of wind turbines in a power system simulations tool. Some of the models used in this project were readily available prior to the project; the development of others is part of the project. The most extensive modelling work deals with the design of the electrical part of the variable speed turbine and its controls. To simulate realistic grid operation the wind turbine models are connected to an aggregated model of the Nordic power system. For that purpose the Nordic power system model, which was available prior to the project, is extended with a realistic feeder configuration. It is commonly demanded from modern wind turbines, that they must not disconnect in case of transient faults. Therefore, controllers are designed that enable the two turbine types to ride through transient faults. With these transient fault controllers the wind turbines can stay connected to the grid, such that their generation capacity is

  5. Scaffold expulsion and genome packaging trigger stabilization of herpes simplex virus capsids

    NARCIS (Netherlands)

    Roos, W.H.; Radtke, K.; Kniesmeijer, E.G.R.; Geertsema, H.J.; Sodeik, B.; Wuite, G.J.L.

    2009-01-01

    Herpes simplex virus type 1 (HSV1) capsids undergo extensive structural changes during maturation and DNA packaging. As a result, they become more stable and competent for nuclear egress. To further elucidate this stabilization process, we used biochemical and nanoindentation approaches to analyze

  6. Scaffold expulsion and genome packaging trigger stabilization of Herpes Simplex Virus capsids

    NARCIS (Netherlands)

    Roos, W.H.; Radtke, K.; Kniesmeijer, E.; Geertsema, H.J.; Sodeik, B.; Wuite, G.J.L.

    2009-01-01

    Herpes simplex virus type 1 (HSV1) capsids undergo extensive structural changes during maturation and DNA packaging. As a result, they become more stable and competent for nuclear egress. To further elucidate this stabilization process, we used biochemical and nanoindentation approaches to analyze

  7. Stability Assessment and Tuning of an Adaptively Augmented Classical Controller for Launch Vehicle Flight Control

    Science.gov (United States)

    VanZwieten, Tannen; Zhu, J. Jim; Adami, Tony; Berry, Kyle; Grammar, Alex; Orr, Jeb S.; Best, Eric A.

    2014-01-01

    Recently, a robust and practical adaptive control scheme for launch vehicles [ [1] has been introduced. It augments a classical controller with a real-time loop-gain adaptation, and it is therefore called Adaptive Augmentation Control (AAC). The loop-gain will be increased from the nominal design when the tracking error between the (filtered) output and the (filtered) command trajectory is large; whereas it will be decreased when excitation of flex or sloshing modes are detected. There is a need to determine the range and rate of the loop-gain adaptation in order to retain (exponential) stability, which is critical in vehicle operation, and to develop some theoretically based heuristic tuning methods for the adaptive law gain parameters. The classical launch vehicle flight controller design technics are based on gain-scheduling, whereby the launch vehicle dynamics model is linearized at selected operating points along the nominal tracking command trajectory, and Linear Time-Invariant (LTI) controller design techniques are employed to ensure asymptotic stability of the tracking error dynamics, typically by meeting some prescribed Gain Margin (GM) and Phase Margin (PM) specifications. The controller gains at the design points are then scheduled, tuned and sometimes interpolated to achieve good performance and stability robustness under external disturbances (e.g. winds) and structural perturbations (e.g. vehicle modeling errors). While the GM does give a bound for loop-gain variation without losing stability, it is for constant dispersions of the loop-gain because the GM is based on frequency-domain analysis, which is applicable only for LTI systems. The real-time adaptive loop-gain variation of the AAC effectively renders the closed-loop system a time-varying system, for which it is well-known that the LTI system stability criterion is neither necessary nor sufficient when applying to a Linear Time-Varying (LTV) system in a frozen-time fashion. Therefore, a

  8. Nuclear alpha spectrin: Critical roles in DNA interstrand cross-link repair and genomic stability

    OpenAIRE

    Lambert, Muriel W

    2016-01-01

    Non-erythroid alpha spectrin (?IISp) is a structural protein which we have shown is present in the nucleus of human cells. It interacts with a number of nuclear proteins such as actin, lamin, emerin, chromatin remodeling factors, and DNA repair proteins. ?IISp?s interaction with DNA repair proteins has been extensively studied. We have demonstrated that nuclear ?IISp is critical in DNA interstrand cross-link (ICL) repair in S phase, in both genomic (non-telomeric) and telomeric DNA, and in ma...

  9. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability

    DEFF Research Database (Denmark)

    Haahr, Peter; Borgermann, Nikoline; Guo, Xiaohu

    2018-01-01

    Deubiquitylating enzymes (DUBs) enhance the dynamics of the versatile ubiquitin (Ub) code by reversing and regulating cellular ubiquitylation processes at multiple levels. Here we discovered that the uncharacterized human protein ZUFSP (zinc finger with UFM1-specific peptidase domain protein/C6orf......113/ZUP1), which has been annotated as a potentially inactive UFM1 protease, and its fission yeast homolog Mug105 define a previously unrecognized class of evolutionarily conserved cysteine protease DUBs. Human ZUFSP selectively interacts with and cleaves long K63-linked poly-Ub chains by means...... establish ZUFSP as a new type of linkage-selective cysteine peptidase DUB with a role in genome maintenance pathways....

  10. Vehicle handling and stability control by the cooperative control of 4WS and DYC

    Science.gov (United States)

    Shen, Huan; Tan, Yun-Sheng

    2017-07-01

    This paper proposes an integrated control system that cooperates with the four-wheel steering (4WS) and direct yaw moment control (DYC) to improve the vehicle handling and stability. The design works of the four-wheel steering and DYC control are based on sliding mode control. The integration control system produces the suitable 4WS angle and corrective yaw moment so that the vehicle tracks the desired yaw rate and sideslip angle. Considering the change of the vehicle longitudinal velocity that means the comfort of driving conditions, both the driving torque and braking torque are used to generate the corrective yaw moment. Simulation results show the effectiveness of the proposed control algorithm.

  11. Power system stabilizer control for wind power to enhance power system stability

    OpenAIRE

    Domínguez García, José Luís; Gomis Bellmunt, Oriol; Bianchi, Fernando Daniel; Sumper, Andreas

    2011-01-01

    The paper presents a small signal stability analysis for power systems with wind farm interaction. Power systems have damping oscillation modes that can be excited by disturbance or fault in the grid. The power converters of the wind farms can be used to reduce these oscillations and make the system more stable. These ideas are explored to design a power system stabilized (PSS) for a network with conventional generators and a wind farm in order to increase the damping of the oscillation...

  12. Roles of POLD4, smallest subunit of DNA polymerase {delta}, in nuclear structures and genomic stability of human cells

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Qin Miao [Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya (Japan); Akashi, Tomohiro [Division of Molecular Mycology and Medicine, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya (Japan); Masuda, Yuji; Kamiya, Kenji [Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553 (Japan); Takahashi, Takashi [Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya (Japan); Suzuki, Motoshi, E-mail: msuzuki@med.nagoya-u.ac.jp [Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya (Japan)

    2010-01-01

    Mammalian DNA polymerase {delta} (pol {delta}) is essential for DNA replication, though the functions of this smallest subunit of POLD4 have been elusive. We investigated pol {delta} activities in vitro and found that it was less active in the absence of POLD4, irrespective of the presence of the accessory protein PCNA. shRNA-mediated reduction of POLD4 resulted in a marked decrease in colony formation activity by Calu6, ACC-LC-319, and PC-10 cells. We also found that POLD4 reduction was associated with an increased population of karyomere-like cells, which may be an indication of DNA replication stress and/or DNA damage. The karyomere-like cells retained an ability to progress through the cell cycle, suggesting that POLD4 reduction induces modest genomic instability, while allowing cells to grow until DNA damage reaches an intolerant level. Our results indicate that POLD4 is required for the in vitro pol {delta} activity, and that it functions in cell proliferation and maintenance of genomic stability of human cells.

  13. Roles of POLD4, smallest subunit of DNA polymerase δ, in nuclear structures and genomic stability of human cells

    International Nuclear Information System (INIS)

    Huang, Qin Miao; Akashi, Tomohiro; Masuda, Yuji; Kamiya, Kenji; Takahashi, Takashi; Suzuki, Motoshi

    2010-01-01

    Mammalian DNA polymerase δ (pol δ) is essential for DNA replication, though the functions of this smallest subunit of POLD4 have been elusive. We investigated pol δ activities in vitro and found that it was less active in the absence of POLD4, irrespective of the presence of the accessory protein PCNA. shRNA-mediated reduction of POLD4 resulted in a marked decrease in colony formation activity by Calu6, ACC-LC-319, and PC-10 cells. We also found that POLD4 reduction was associated with an increased population of karyomere-like cells, which may be an indication of DNA replication stress and/or DNA damage. The karyomere-like cells retained an ability to progress through the cell cycle, suggesting that POLD4 reduction induces modest genomic instability, while allowing cells to grow until DNA damage reaches an intolerant level. Our results indicate that POLD4 is required for the in vitro pol δ activity, and that it functions in cell proliferation and maintenance of genomic stability of human cells.

  14. Aqueous clay suspensions stabilized by alginate fluid gels for coal spontaneous combustion prevention and control.

    Science.gov (United States)

    Qin, Botao; Ma, Dong; Li, Fanglei; Li, Yong

    2017-11-01

    We have developed aqueous clay suspensions stabilized by alginate fluid gels (AFG) for coal spontaneous combustion prevention and control. Specially, this study aimed to characterize the effect of AFG on the microstructure, static and dynamic stability, and coal fire inhibition performances of the prepared AFG-stabilized clay suspensions. Compared with aqueous clay suspensions, the AFG-stabilized clay suspensions manifest high static and dynamic stability, which can be ascribed to the formation of a robust three-dimensional gel network by AFG. The coal acceleration oxidation experimental results show that the prepared AFG-stabilized clay suspensions can improve the coal thermal stability and effectively inhibit the coal spontaneous oxidation process by increasing crossing point temperature (CPT) and reducing CO emission. The prepared low-cost and nontoxic AFG-stabilized clay suspensions, exhibiting excellent coal fire extinguishing performances, indicate great application potentials in coal spontaneous combustion prevention and control.

  15. HPLC FOR CONTROL STABILITY OF QUERCETIN INJECTABLE DOSAGE FORM

    Directory of Open Access Journals (Sweden)

    Martynov AV

    2016-12-01

    retention time and spectral relations in the UV region from the database of DB-2003 spectrums. HPLC analysis results show that the quercetin is stable in ampouled form under autoclave and storage and freshly diluted quercetin concentrate for infusion are identical. Quercetin aqueous solution which was stored at room temperature for 14 days in the light, turned out to be unstable. It was found that aqueous solutions of polysorbate-80 was full hydrolyzed to the initial compounds. Conclusions In this work the ability of quercetin’s perspective concentrates to be stable were checked. The stability of concentrates was determined by HPLC chromatograph "Millichrome A - 02» (SiChrom, Knauer. It is shown that the HPLC methods can be used to establish the smallest difference in the samples. The quercetin’s non-aqueous concentrate is capable of withstanding retorting and remains in standard indestructible state in nonaqueous media (glycerol, ethanol, polysorbate 80. Quercetin is unstable in aqueous solutions and are destroyed during prolonged storage. HPLC- chromatogram is presented in the article and show that gradient HPLC with UV- detection can be used for quality control of quercetin.

  16. Scaffold expulsion and genome packaging trigger stabilization of herpes simplex virus capsids

    Science.gov (United States)

    Roos, Wouter H.; Radtke, Kerstin; Kniesmeijer, Edward; Geertsema, Hylkje; Sodeik, Beate; Wuite, Gijs J. L.

    2009-01-01

    Herpes simplex virus type 1 (HSV1) capsids undergo extensive structural changes during maturation and DNA packaging. As a result, they become more stable and competent for nuclear egress. To further elucidate this stabilization process, we used biochemical and nanoindentation approaches to analyze the structural and mechanical properties of scaffold-containing (B), empty (A), and DNA-containing (C) nuclear capsids. Atomic force microscopy experiments revealed that A and C capsids were mechanically indistinguishable, indicating that the presence of DNA does not account for changes in mechanical properties during capsid maturation. Despite having the same rigidity, the scaffold-containing B capsids broke at significantly lower forces than A and C capsids. An extraction of pentons with guanidine hydrochloride (GuHCl) increased the flexibility of all capsids. Surprisingly, the breaking forces of the modified A and C capsids dropped to similar values as those of the GuHCl-treated B capsids, indicating that mechanical reinforcement occurs at the vertices. Nonetheless, it also showed that HSV1 capsids possess a remarkable structural integrity that was preserved after removal of pentons. We suggest that HSV1 capsids are stabilized after removal of the scaffold proteins, and that this stabilization is triggered by the packaging of DNA, but independent of the actual presence of DNA. PMID:19487681

  17. Nonlinear power flow feedback control for improved stability and performance of airfoil sections

    Science.gov (United States)

    Wilson, David G.; Robinett, III, Rush D.

    2013-09-03

    A computer-implemented method of determining the pitch stability of an airfoil system, comprising using a computer to numerically integrate a differential equation of motion that includes terms describing PID controller action. In one model, the differential equation characterizes the time-dependent response of the airfoil's pitch angle, .alpha.. The computer model calculates limit-cycles of the model, which represent the stability boundaries of the airfoil system. Once the stability boundary is known, feedback control can be implemented, by using, for example, a PID controller to control a feedback actuator. The method allows the PID controller gain constants, K.sub.I, K.sub.p, and K.sub.d, to be optimized. This permits operation closer to the stability boundaries, while preventing the physical apparatus from unintentionally crossing the stability boundaries. Operating closer to the stability boundaries permits greater power efficiencies to be extracted from the airfoil system.

  18. Finite-Time Stabilization and Adaptive Control of Memristor-Based Delayed Neural Networks.

    Science.gov (United States)

    Wang, Leimin; Shen, Yi; Zhang, Guodong

    Finite-time stability problem has been a hot topic in control and system engineering. This paper deals with the finite-time stabilization issue of memristor-based delayed neural networks (MDNNs) via two control approaches. First, in order to realize the stabilization of MDNNs in finite time, a delayed state feedback controller is proposed. Then, a novel adaptive strategy is applied to the delayed controller, and finite-time stabilization of MDNNs can also be achieved by using the adaptive control law. Some easily verified algebraic criteria are derived to ensure the stabilization of MDNNs in finite time, and the estimation of the settling time functional is given. Moreover, several finite-time stability results as our special cases for both memristor-based neural networks (MNNs) without delays and neural networks are given. Finally, three examples are provided for the illustration of the theoretical results.Finite-time stability problem has been a hot topic in control and system engineering. This paper deals with the finite-time stabilization issue of memristor-based delayed neural networks (MDNNs) via two control approaches. First, in order to realize the stabilization of MDNNs in finite time, a delayed state feedback controller is proposed. Then, a novel adaptive strategy is applied to the delayed controller, and finite-time stabilization of MDNNs can also be achieved by using the adaptive control law. Some easily verified algebraic criteria are derived to ensure the stabilization of MDNNs in finite time, and the estimation of the settling time functional is given. Moreover, several finite-time stability results as our special cases for both memristor-based neural networks (MNNs) without delays and neural networks are given. Finally, three examples are provided for the illustration of the theoretical results.

  19. Intensity and frequency stabilization of a laser diode by simultaneously controlling its temperature and current

    Science.gov (United States)

    Mu, Weiwei; Hu, Zhaohui; Wang, Jing; Zhou, Binquan

    2017-10-01

    Nuclear magnetic resonance gyroscope (NMRG) detects the angular velocity of the vehicle utilizing the interaction between the laser beam and the alkali metal atoms along with the noble gas atoms in the alkali vapor cell. In order to reach high precision inertial measurement target, semiconductor laser in NMRG should have good intensity and frequency stability. Generally, laser intensity and frequency are stabilized separately. In this paper, a new method to stabilize laser intensity and frequency simultaneously with double-loop feedback control is presented. Laser intensity is stabilized to the setpoint value by feedback control of laser diode's temperature. Laser frequency is stabilized to the Doppler absorption peak by feedback control of laser diode's current. The feedback control of current is a quick loop, hence the laser frequency stabilize quickly. The feedback control of temperature is a slow loop, hence the laser intensity stabilize slowly. With the feedback control of current and temperature, the laser intensity and frequency are stabilized finally. Additionally, the dependence of laser intensity and frequency on laser diode's current and temperature are analyzed, which contributes to choose suitable operating range for the laser diode. The advantage of our method is that the alkali vapor cell used for stabilizing laser frequency is the same one as the cell used for NMRG to operate, which helps to miniaturize the size of NMRG prototype. In an 8-hour continuous measurement, the long-term stability of laser intensity and frequency increased by two orders of magnitude and one order of magnitude respectively.

  20. Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1.

    Science.gov (United States)

    Lee, Seung Baek; Kim, Jung Jin; Nam, Hyun-Ja; Gao, Bowen; Yin, Ping; Qin, Bo; Yi, Sang-Yeop; Ham, Hyoungjun; Evans, Debra; Kim, Sun-Hyun; Zhang, Jun; Deng, Min; Liu, Tongzheng; Zhang, Haoxing; Billadeau, Daniel D; Wang, Liewei; Giaime, Emilie; Shen, Jie; Pang, Yuan-Ping; Jen, Jin; van Deursen, Jan M; Lou, Zhenkun

    2015-10-01

    Mutations in the E3 ubiquitin ligase Parkin have been linked to familial Parkinson's disease. Parkin has also been implicated in mitosis through mechanisms that are unclear. Here we show that Parkin interacts with anaphase promoting complex/cyclosome (APC/C) coactivators Cdc20 and Cdh1 to mediate the degradation of several key mitotic regulators independent of APC/C. We demonstrate that ordered progression through mitosis is orchestrated by two distinct E3 ligases through the shared use of Cdc20 and Cdh1. Furthermore, Parkin is phosphorylated and activated by polo-like kinase 1 (Plk1) during mitosis. Parkin deficiency results in overexpression of its substrates, mitotic defects, genomic instability, and tumorigenesis. These results suggest that the Parkin-Cdc20/Cdh1 complex is an important regulator of mitosis. Copyright © 2015 Elsevier Inc. All rights reserved.

  1. Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.

    Directory of Open Access Journals (Sweden)

    Domenico Iuso

    Full Text Available The unprecedented decline of biodiversity worldwide is urging scientists to collect and store biological material from seriously threatened animals, including large mammals. Lyophilization is being explored as a low-cost system for storage in bio-banks of cells that might be used to expand or restore endangered or extinct species through the procedure of Somatic Cell Nuclear Transfer (SCNT. Here we report that the genome is intact in about 60% of lyophylized sheep lymphocytes, whereas DNA damage occurs randomly in the remaining 40%. Remarkably, lyophilized nuclei injected into enucleated oocytes are repaired by a robust DNA repairing activity of the oocytes, and show normal developmental competence. Cloned embryos derived from lyophylized cells exhibited chromosome and cellular composition comparable to those of embryos derived from fresh donor cells. These findings support the feasibility of lyophylization as a storage procedure of mammalian cells to be used for SCNT.

  2. Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.

    Science.gov (United States)

    Iuso, Domenico; Czernik, Marta; Di Egidio, Fiorella; Sampino, Silvestre; Zacchini, Federica; Bochenek, Michal; Smorag, Zdzislaw; Modlinski, Jacek A; Ptak, Grazyna; Loi, Pasqualino

    2013-01-01

    The unprecedented decline of biodiversity worldwide is urging scientists to collect and store biological material from seriously threatened animals, including large mammals. Lyophilization is being explored as a low-cost system for storage in bio-banks of cells that might be used to expand or restore endangered or extinct species through the procedure of Somatic Cell Nuclear Transfer (SCNT). Here we report that the genome is intact in about 60% of lyophylized sheep lymphocytes, whereas DNA damage occurs randomly in the remaining 40%. Remarkably, lyophilized nuclei injected into enucleated oocytes are repaired by a robust DNA repairing activity of the oocytes, and show normal developmental competence. Cloned embryos derived from lyophylized cells exhibited chromosome and cellular composition comparable to those of embryos derived from fresh donor cells. These findings support the feasibility of lyophylization as a storage procedure of mammalian cells to be used for SCNT.

  3. Pitch Motion Stabilization by Propeller Speed Control Using Statistical Controller Design

    DEFF Research Database (Denmark)

    Nakatani, Toshihiko; Blanke, Mogens; Galeazzi, Roberto

    2006-01-01

    This paper describes dynamics analysis of a small training boat and a possibility of ship pitch stabilization by control of propeller speed. After upgrading the navigational system of an actual small training boat, in order to identify the model of the ship, the real data collected by sea trials...... were used for statistical analysis and system identification. This analysis shows that the pitching motion is indeed influenced by engine speed and it is suggested that there exists a possibility of reducing the pitching motion by properly controlling the engine throttle. Based on this observation...

  4. [RTEL1 (regulator of telomere elongation helicase 1), a DNA helicase essential for genome stability].

    Science.gov (United States)

    Le Guen, Tangui; Jullien, Laurent; Schertzer, Mike; Lefebvre, Axelle; Kermasson, Laetitia; de Villartay, Jean-Pierre; Londoño-Vallejo, Arturo; Revy, Patrick

    2013-12-01

    RTEL1 (regulator of telomere length helicase 1) is a DNA helicase that has been identified more than 10 years ago. Many works since, mainly in the nematode Caenorhabditis elegans and the mouse, have highlighted its role in chromosomal stability, maintenance of telomere length, and DNA repair. Recently, four laboratories have characterized RTEL1 mutations in patients with dyskeratosis congenita (DC) and Hoyeraal-Hreidarsson (HH) syndrome, a rare and severe variant of DC. We here summarize the current knowledge on RTEL1 and discuss the possible other functions that RTEL1 could play. © 2013 médecine/sciences – Inserm.

  5. ATMP-stabilized iron nanoparticles: chelator-controlled nanoparticle synthesis

    Science.gov (United States)

    Greenlee, Lauren F.; Rentz, Nikki S.

    2014-11-01

    In this study, we characterize iron nanoparticles synthesized in water in the presence of a phosphonate chelator, amino tris(methylene phosphonic acid) (ATMP) for a range of molar ratios of ATMP to iron. An increase in the molar ratio from 0.05 to 0.8 decreases nanoparticle size from approximately 150 nm to less than 10 nm. Zeta potential measurements were used to evaluate colloidal stability. Zeta potential values varied as a function of pH, and zeta potential values decreased with increasing pH. At lower molar ratios of ATMP to iron, the zeta potential varied between 15 and -40 mV, passing through an isoelectric point at pH 7.5. At higher ratios, the zeta potential was negative across the measured pH range of 2-12 and varied from -2 to -55 mV. Diffraction analysis indicates that ATMP-stabilized iron nanoparticles may have a nano-crystalline structure, potentially with regions of amorphous iron. Characterization results of ATMP-stabilized iron nanoparticles are compared to results obtained for carboxymethyl cellulose (CMC)-stabilized iron nanoparticles. CMC stabilization caused similar peak broadening in diffraction spectra as for ATMP, suggesting similar nano-crystalline/amorphous structure; however, an increase in the molar ratio of CMC to iron did not cause the same reduction in nanoparticle size as was observed for ATMP-stabilized iron nanoparticles.

  6. Design of Simulation Product for Stability of Electric Power System Using Power System Stabilizer and Optimal Control

    Science.gov (United States)

    Junaidi, Agus; Hamid, K. Abdul

    2018-03-01

    This paper will discuss the use of optimal control and Power System Stabilizer (PSS) in improving the oscillation of electric power system. Oscillations in the electric power system can occur due to the sudden release of the load (Switcing-Off). The oscillation of an unstable system for a long time causes the equipment to work in an interruption. To overcome this problem, a control device is required that can work effectively in repairing the oscillation. The power system is modeled from the Single Machine Infinite Bus Model (SMIB). The state space equation is used to mathematically model SMIB. SMIB system which is a plant will be formed togetherness state variables (State-Space), using riccati equation then determined the optimal gain as controller plant. Plant is also controlled by Power Stabilizer System using phase compensation method. Using Matlab Software based simulation will be observed response of rotor speed change and rotor angle change for each of the two controlling methods. Simulation results using the Simulink-MATLAB 6.1 software will compare the analysis of the plant state in Open loop state and use the controller. The simulation response shows that the optimal control and PSS can improve the stability of the power system in terms of acceleration to achieve settling-time and Over Shoot improvement. From the results of both methods are able to improve system performance.

  7. Stability of a neural predictive controller scheme on a neural model

    DEFF Research Database (Denmark)

    Luther, Jim Benjamin; Sørensen, Paul Haase

    2009-01-01

    In previous works presenting various forms of neural-network-based predictive controllers, the main emphasis has been on the implementation aspects, i.e. the development of a robust optimization algorithm for the controller, which will be able to perform in real time. However, the stability issue....... The resulting controller is tested on a nonlinear pneumatic servo system.......In previous works presenting various forms of neural-network-based predictive controllers, the main emphasis has been on the implementation aspects, i.e. the development of a robust optimization algorithm for the controller, which will be able to perform in real time. However, the stability issue...... has not been addressed specifically for these controllers. On the other hand a number of results concerning the stability of receding horizon controllers on a nonlinear system exist. In this paper we present a proof of stability for a predictive controller controlling a neural network model...

  8. Stabilization

    Directory of Open Access Journals (Sweden)

    Muhammad H. Al-Malack

    2016-07-01

    Full Text Available Fuel oil flyash (FFA produced in power and water desalination plants firing crude oils in the Kingdom of Saudi Arabia is being disposed in landfills, which increases the burden on the environment, therefore, FFA utilization must be encouraged. In the current research, the effect of adding FFA on the engineering properties of two indigenous soils, namely sand and marl, was investigated. FFA was added at concentrations of 5%, 10% and 15% to both soils with and without the addition of Portland cement. Mixtures of the stabilized soils were thoroughly evaluated using compaction, California Bearing Ratio (CBR, unconfined compressive strength (USC and durability tests. Results of these tests indicated that stabilized sand mixtures could not attain the ACI strength requirements. However, marl was found to satisfy the ACI strength requirement when only 5% of FFA was added together with 5% of cement. When the FFA was increased to 10% and 15%, the mixture’s strength was found to decrease to values below the ACI requirements. Results of the Toxicity Characteristics Leaching Procedure (TCLP, which was performed on samples that passed the ACI requirements, indicated that FFA must be cautiously used in soil stabilization.

  9. Stability and performance of propulsion control systems with distributed control architectures and failures

    Science.gov (United States)

    Belapurkar, Rohit K.

    Future aircraft engine control systems will be based on a distributed architecture, in which, the sensors and actuators will be connected to the Full Authority Digital Engine Control (FADEC) through an engine area network. Distributed engine control architecture will allow the implementation of advanced, active control techniques along with achieving weight reduction, improvement in performance and lower life cycle cost. The performance of a distributed engine control system is predominantly dependent on the performance of the communication network. Due to the serial data transmission policy, network-induced time delays and sampling jitter are introduced between the sensor/actuator nodes and the distributed FADEC. Communication network faults and transient node failures may result in data dropouts, which may not only degrade the control system performance but may even destabilize the engine control system. Three different architectures for a turbine engine control system based on a distributed framework are presented. A partially distributed control system for a turbo-shaft engine is designed based on ARINC 825 communication protocol. Stability conditions and control design methodology are developed for the proposed partially distributed turbo-shaft engine control system to guarantee the desired performance under the presence of network-induced time delay and random data loss due to transient sensor/actuator failures. A fault tolerant control design methodology is proposed to benefit from the availability of an additional system bandwidth and from the broadcast feature of the data network. It is shown that a reconfigurable fault tolerant control design can help to reduce the performance degradation in presence of node failures. A T-700 turbo-shaft engine model is used to validate the proposed control methodology based on both single input and multiple-input multiple-output control design techniques.

  10. The conditions for attaining the greatest degree of system stability with strict generator excitation control

    Energy Technology Data Exchange (ETDEWEB)

    Gruzdev, I.A.; Ekimova, M.M.; Truspekova, G.A.

    1982-01-01

    Expressions are derived for an idealized model of a complex electric power system; these expressions define the greatest level of stability of an electric power system and the optimum combination of stabilization factors with automatic excitation control in a single power system. The possibility of increasing the level of stability of an electric power system with simultaneous strict automatic excitation control of the synychronous generators in several power systems is analyzed.

  11. A role of NBS1 in genome stability after double-strand breaks

    International Nuclear Information System (INIS)

    Komatsu, K.; Tauchi, H.; Matsuura, S.; Antoccia, A.

    2003-01-01

    DNA double-strand breaks (DSBs) represent the most serious damage in genome, and hence, the cells correctly repair one DSB generated in a cell. This efficiency of DNA repair could correspond to detect several bp out of 6x10 9 bps in genome. However, it is not yet characterized how DSBs are recognized and repair proteins are accurately recruited to the sites of DSBs. Here, we propose a two-step binding model led by NBS1, gene product defective in Nijmegen Breakage Syndrome. NBS1 physically interacts with histone, rather than damage DNA, by direct binding to γ -H2AX. We demonstrate that the NBS1-binding can occur in the absence of interaction with hMRE11 or BRCA1. NBS1 has no DNA binding region but carries a combination of the fork-head associated (FHA) and the BRCA1 C-terminal domains (BRCT). We show that the FHA/BRCT domain of NBS1 is essential for this physical interaction, since NBS1 lacking this domain failed to bind to γ -H2AX in cells, and a recombinant FHA/BRCT domain alone can bind to recombinant γ -H2AX. Thus, the interaction with γ -H2AX has a crucial role for re-localization of NBS1/hMRE11/hRAD50 nuclease complex to the vicinity of DNA damage. After conversion of this complex to binding to damage DNA, it will initiate DNA repair. When the ability of homologous recombination in chicken NBS cells was assayed by using SCneo reporter plasmid, the homologous recombination was about 100-fold decrease, compared to that of chicken wild cells. However, non-homologous end joining in chicken NBS cells is indistinguishable from those in wild type cells. NBS1 is also involved in S-phase checkpoint through the SMC1 phospohrylation when cells were irradiated with a low dose. As a result, this re-localization of NBS1/hMre11/ hRad50 complex through interaction of NBS1 with histone could be a key step in a two-step binding mechanism for homologous recombination repair and cell-cycle checkpoints

  12. Controllability and stability of primary frequency control from thermostatic loads with delays

    DEFF Research Database (Denmark)

    Ziras, Charalampos; Vrettos, Evangelos; You, Shi

    2017-01-01

    There is an increasing interest in exploiting the flexibility of loads to provide ancillary services to the grid. In this paper we study how response delays and lockout constraints affect the controllability of an aggregation of refrigerators offering primary frequency control (PFC). First we...... examine the effect of delays in PFC provision from an aggregation of refrigerators, using a two-area power system. We propose a framework to systematically address frequency measurement and response delays and we determine safe values for the total delays via simulations. We introduce a controllability...... index to evaluate PFC provision under lockout constraints of refrigerators compressors. We conduct extensive simulations to study the effects of measurement delay, ramping times, lockout durations and rotational inertia on the controllability of the aggregation and system stability. Finally, we discuss...

  13. PID self tuning control based on Mamdani fuzzy logic control for quadrotor stabilization

    Energy Technology Data Exchange (ETDEWEB)

    Priyambodo, Tri Kuntoro, E-mail: mastri@ugm.ac.id; Putra, Agfianto Eko [Aerospace and Aeronautics Electronics Research Group, Universitas Gadjah Mada, Yogyakarta (Indonesia); Department of Computer Science and Electronics, Universitas Gadjah Mada, Yogyakarta (Indonesia); Dharmawan, Andi, E-mail: andi-dharmawan@ugm.ac.id [Department of Computer Science and Electronics, Universitas Gadjah Mada, Yogyakarta (Indonesia)

    2016-02-01

    Quadrotor as one type of UAV have the ability to perform Vertical Take Off and Landing (VTOL). It allows the Quadrotor to be stationary hovering in the air. PID (Proportional Integral Derivative) control system is one of the control methods that are commonly used. It is usually used to optimize the Quadrotor stabilization at least based on the three Eulerian angles (roll, pitch, and yaw) as input parameters for the control system. The three constants of PID can be obtained in various methods. The simplest method is tuning manually. This method has several weaknesses. For example if the three constants are not exact, the resulting response will deviate from the desired result. By combining the methods of PID with fuzzy logic systems where human expertise is implemented into the machine language is expected to further optimize the control system.

  14. Draft Genome Sequence of Bacillus velezensis B6, a Rhizobacterium That Can Control Plant Diseases.

    Science.gov (United States)

    Gao, Yu-Han; Guo, Rong-Jun; Li, Shi-Dong

    2018-03-22

    The draft genome of Bacillus velezensis strain B6, a rhizobacterium with good biocontrol performance isolated from soil in China, was sequenced. The assembly comprises 32 scaffolds with a total size of 3.88 Mb. Gene clusters coding either ribosomally encoded bacteriocins or nonribosomally encoded antimicrobial polyketides and lipopeptides in the genome may contribute to plant disease control. Copyright © 2018 Gao et al.

  15. Strategies for Voltage Control and Transient Stability Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Hiskens, Ian A.

    2013-09-25

    As wind generation grows, its influence on power system performance will becoming increasingly noticeable. Wind generation di ffers from traditional forms of generation in numerous ways though, motivating the need to reconsider the usual approaches to power system assessment and performance enhancement. The project has investigated the impact of wind generation on transient stability and voltage control, identifying and addressing issues at three distinct levels of the power system: 1) at the device level, the physical characteristics of wind turbine generators (WTGs) are quite unlike those of synchronous machines, 2) at the wind-farm level, the provision of reactive support is achieved through coordination of numerous dissimilar devices, rather than straightforward generator control, and 3) from a systems perspective, the location of wind-farms on the sub-transmission network, coupled with the variability inherent in their power output, can cause complex voltage control issues. The project has sought to develop a thorough understanding of the dynamic behaviour of type-3 WTGs, and in particular the WECC generic model. The behaviour of such models is governed by interactions between the continuous dynamics of state variables and discrete events associated with limits. It was shown that these interactions can be quite complex, and may lead to switching deadlock that prevents continuation of the trajectory. Switching hysteresis was proposed for eliminating deadlock situations. Various type-3 WTG models include control blocks that duplicate integrators. It was shown that this leads to non-uniqueness in the conditions governing steady-state, and may result in pre- and post-disturbance equilibria not coinciding. It also gives rise to a zero eigenvalue in the linearized WTG model. In order to eliminate the anomalous behaviour revealed through this investigation, WECC has now released a new generic model for type-3 WTGs. Wind-farms typically incorporate a variety of

  16. Genomics in Public Health: Perspective from the Office of Public Health Genomics at the Centers for Disease Control and Prevention (CDC

    Directory of Open Access Journals (Sweden)

    Ridgely Fisk Green

    2015-09-01

    Full Text Available The national effort to use genomic knowledge to save lives is gaining momentum, as illustrated by the inclusion of genomics in key public health initiatives, including Healthy People 2020, and the recent launch of the precision medicine initiative. The Office of Public Health Genomics (OPHG at the Centers for Disease Control and Prevention (CDC partners with state public health departments and others to advance the translation of genome-based discoveries into disease prevention and population health. To do this, OPHG has adopted an “identify, inform, and integrate” model: identify evidence-based genomic applications ready for implementation, inform stakeholders about these applications, and integrate these applications into public health at the local, state, and national level. This paper addresses current and future work at OPHG for integrating genomics into public health programs.

  17. Genomics in Public Health: Perspective from the Office of Public Health Genomics at the Centers for Disease Control and Prevention (CDC).

    Science.gov (United States)

    Green, Ridgely Fisk; Dotson, W David; Bowen, Scott; Kolor, Katherine; Khoury, Muin J

    2015-01-01

    The national effort to use genomic knowledge to save lives is gaining momentum, as illustrated by the inclusion of genomics in key public health initiatives, including Healthy People 2020, and the recent launch of the precision medicine initiative. The Office of Public Health Genomics (OPHG) at the Centers for Disease Control and Prevention (CDC) partners with state public health departments and others to advance the translation of genome-based discoveries into disease prevention and population health. To do this, OPHG has adopted an "identify, inform, and integrate" model: identify evidence-based genomic applications ready for implementation, inform stakeholders about these applications, and integrate these applications into public health at the local, state, and national level. This paper addresses current and future work at OPHG for integrating genomics into public health programs.

  18. STABILITY CONTROL OF ELEVATING-TRANSFER VEHICLES IN THE CONSTRUCTION

    Directory of Open Access Journals (Sweden)

    Zhadanovskiy Boris Vasil’evich

    2016-05-01

    Full Text Available The underground space is widely used in the construction in big cities of the Russian Federation. These works need the use of elevating-transfer vehicles. In this case the requirements of norms and regulations on operating safety should be strictly observed, because their breach often leads to emergency situations and injuries. The organizational and technological solutions when developing the design documentation and executing construction and assembly works should be primarily based on the stability of lifting facilities. The author states the requirements to installation of lifting tackles (cranes. The features of their installation in different operation conditions on construction sites are described. The crane stability depends on many different indicators, which are considered by the author. The calculation algorithms of crane stability are offered.

  19. Settlement Control of Soft Ground using Cement-Ricehusk Stabilization

    Directory of Open Access Journals (Sweden)

    Mokhtar M.

    2012-01-01

    Full Text Available Cement is widely used for improvement of soft soils, but financial and environmental concerns are causing genuine concerns to all parties, leading to the quest for alternative and effective stabilizers. Ricehusk is an agricultural waste in Malaysia, commonly disposed of by open burning or dumping in landfills. Considering that the ashes derived from ricehusk are pozzolanic in nature, there is a possibility that a cement-ricehusk mixture could effectively improve soft soils with reduced cement dosage. This study examines the mixture’s effectiveness by monitoring the settlement reduction in a clay soil. Standard oedometer tests were carried out on a soft marine clay sample admixed with cement-ricehusk. Test specimens contained 0-10% cement and 0-5% of ricehusk respectively, and were left to cure for either seven or 28 days. The stabilized specimens were observed to undergo significant reduction in compressibility, verifying the potential of cement-ricehusk as an alternative soft soil stabilizer.

  20. On a program manifold’s stability of one contour automatic control systems

    Directory of Open Access Journals (Sweden)

    Zumatov S. S.

    2017-12-01

    Full Text Available Methodology of analysis of stability is expounded to the one contour systems automatic control feedback in the presence of non-linearities. The methodology is based on the use of the simplest mathematical models of the nonlinear controllable systems. Stability of program manifolds of one contour automatic control systems is investigated. The sufficient conditions of program manifold’s absolute stability of one contour automatic control systems are obtained. The Hurwitz’s angle of absolute stability was determined. The sufficient conditions of program manifold’s absolute stability of control systems by the course of plane in the mode of autopilot are obtained by means Lyapunov’s second method.

  1. Optimal supplementary frequency controller design using the wind farm frequency model and controller parameters stability region.

    Science.gov (United States)

    Toulabi, Mohammadreza; Bahrami, Shahab; Ranjbar, Ali Mohammad

    2018-03-01

    In most of the existing studies, the frequency response in the variable speed wind turbines (VSWTs) is simply realized by changing the torque set-point via appropriate inputs such as frequency deviations signal. However, effective dynamics and systematic process design have not been comprehensively discussed yet. Accordingly, this paper proposes a proportional-derivative frequency controller and investigates its performance in a wind farm consisting of several VSWTs. A band-pass filter is deployed before the proposed controller to avoid responding to either steady state frequency deviations or high rate of change of frequency. To design the controller, the frequency model of the wind farm is first characterized. The proposed controller is then designed based on the obtained open loop system. The stability region associated with the controller parameters is analytically determined by decomposing the closed-loop system's characteristic polynomial into the odd and even parts. The performance of the proposed controller is evaluated through extensive simulations in MATLAB/Simulink environment in a power system comprising a high penetration of VSWTs equipped with the proposed controller. Finally, based on the obtained feasible area and appropriate objective function, the optimal values associated with the controller parameters are determined using the genetic algorithm (GA). Copyright © 2018 ISA. Published by Elsevier Ltd. All rights reserved.

  2. A comprehensive gaze stabilization controller based on cerebellar internal models

    DEFF Research Database (Denmark)

    Vannucci, Lorenzo; Falotico, Egidio; Tolu, Silvia

    2017-01-01

    . The VOR works in conjunction with the opto-kinetic reflex (OKR), which is a visual feedback mechanism that allows to move the eye at the same speed as the observed scene. Together they keep the image stationary on the retina. In this work we implement on a humanoid robot a model of gaze stabilization...... based on the coordination of VCR and VOR and OKR. The model, inspired by neuroscientific cerebellar theories, is provided with learning and adaptation capabilities based on internal models. We present the results for the gaze stabilization model on three sets of experiments conducted on the SABIAN robot...

  3. Replication protein A, the laxative that keeps DNA regular: The importance of RPA phosphorylation in maintaining genome stability.

    Science.gov (United States)

    Byrne, Brendan M; Oakley, Gregory G

    2018-04-20

    The eukaryotic ssDNA-binding protein, Replication protein A (RPA), was first discovered almost three decades ago. Since then, much progress has been made to elucidate the critical roles for RPA in DNA metabolic pathways that help promote genomic stability. The canonical RPA heterotrimer (RPA1-3) is an essential coordinator of DNA metabolism that interacts with ssDNA and numerous protein partners to coordinate its roles in DNA replication, repair, recombination and telomere maintenance. An alternative form of RPA, termed aRPA, is formed by a complex of RPA4 with RPA1 and RPA3. aRPA is expressed differentially in cells compared to canonical RPA and has been shown to inhibit canonical RPA function while allowing for regular maintenance of cell viability. Interestingly, while aRPA is defective in DNA replication and cell cycle progression, it was shown to play a supporting role in nucleotide excision repair and recombination. The binding domains of canonical RPA interact with a growing number of partners involved in numerous genome maintenance processes. The protein interactions of the RPA-ssDNA complex are not only governed by competition between the binding proteins but also by post-translation modifications such as phosphorylation. Phosphorylation of RPA2 is an important post-translational modification of the RPA complex, and is essential for directing context-specific functions of the RPA complex in the DNA damage response. Due to the importance of RPA in cellular metabolism, it was identified as an appealing target for chemotherapeutic drug development that could be used in future cancer treatment regimens. Copyright © 2018 Elsevier Ltd. All rights reserved.

  4. Stabilization and tracking controller for a class of nonlinear discrete-time systems

    International Nuclear Information System (INIS)

    Sharma, B.B.; Kar, I.N.

    2011-01-01

    Highlights: → We present recursive design of stabilizing controller for nonlinear discrete-time systems. → Problem of stabilizing and tracking control of single link manipulator system is addressed. → We extend the proposed results to output tracking problems. → The proposed methodology is applied satisfactorily to discrete-time chaotic maps. - Abstract: In this paper, stabilization and tracking control problem for parametric strict feedback class of discrete time systems is addressed. Recursive design of control function based on contraction theory framework is proposed instead of traditional Lyapunov based method. Explicit structure of controller is derived for the addressed class of nonlinear discrete-time systems. Conditions for exponential stability of system states are derived in terms of controller parameters. At each stage of recursive procedure a specific structure of Jacobian matrix is ensured so as to satisfy conditions of stability. The closed loop dynamics in this case remains nonlinear in nature. The proposed algorithm establishes global stability results in quite a simple manner as it does not require formulation of error dynamics. Problem of stabilization and output tracking control in case of single link manipulator system with actuator dynamics is analyzed using the proposed strategy. The proposed results are further extended to stabilization of discrete time chaotic systems. Numerical simulations presented in the end show the effectiveness of the proposed approach.

  5. CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability

    DEFF Research Database (Denmark)

    Martinez, Virginia; Lauritsen, Ida; Hobel, Tonja

    2017-01-01

    Interference with genes is the foundation of reverse genetics and is key to manipulation of living cells for biomedical and biotechnological applications. However, classical genetic knockout and transcriptional knockdown technologies have different drawbacks and offer no control over existing pro...

  6. The beauty of simple adaptive control and new developments in nonlinear systems stability analysis

    Energy Technology Data Exchange (ETDEWEB)

    Barkana, Itzhak, E-mail: ibarkana@gmail.com [BARKANA Consulting, Ramat Hasharon (Israel)

    2014-12-10

    Although various adaptive control techniques have been around for a long time and in spite of successful proofs of stability and even successful demonstrations of performance, the eventual use of adaptive control methodologies in practical real world systems has met a rather strong resistance from practitioners and has remained limited. Apparently, it is difficult to guarantee or even understand the conditions that can guarantee stable operations of adaptive control systems under realistic operational environments. Besides, it is difficult to measure the robustness of adaptive control system stability and allow it to be compared with the common and widely used measure of phase margin and gain margin that is utilized by present, mainly LTI, controllers. Furthermore, customary stability analysis methods seem to imply that the mere stability of adaptive systems may be adversely affected by any tiny deviation from the pretty idealistic and assumably required stability conditions. This paper first revisits the fundamental qualities of customary direct adaptive control methodologies, in particular the classical Model Reference Adaptive Control, and shows that some of their basic drawbacks have been addressed and eliminated within the so-called Simple Adaptive Control methodology. Moreover, recent developments in the stability analysis methods of nonlinear systems show that prior conditions that were customarily assumed to be needed for stability are only apparent and can be eliminated. As a result, sufficient conditions that guarantee stability are clearly stated and lead to similarly clear proofs of stability. As many real-world applications show, once robust stability of the adaptive systems can be guaranteed, the added value of using Add-On Adaptive Control along with classical Control design techniques is pushing the desired performance beyond any previous limits.

  7. Complete Genome Sequence of the Quality Control Strain Staphylococcus aureus subsp. aureus ATCC 25923.

    Science.gov (United States)

    Treangen, Todd J; Maybank, Rosslyn A; Enke, Sana; Friss, Mary Beth; Diviak, Lynn F; Karaolis, David K R; Koren, Sergey; Ondov, Brian; Phillippy, Adam M; Bergman, Nicholas H; Rosovitz, M J

    2014-11-06

    Staphylococcus aureus subsp. aureus ATCC 25923 is commonly used as a control strain for susceptibility testing to antibiotics and as a quality control strain for commercial products. We present the completed genome sequence for the strain, consisting of the chromosome and a 27.5-kb plasmid. Copyright © 2014 Treangen et al.

  8. DC Microgrids–Part I: A Review of Control Strategies and Stabilization Techniques

    Energy Technology Data Exchange (ETDEWEB)

    Dragicevic, Tomislav; Lu, Xiaonan; Vasquez, Juan; Guerrero, Josep

    2015-01-01

    This paper presents a review of control strategies, stability analysis, and stabilization techniques for dc microgrids (MGs). Overall control is systematically classified into local and coordinated control levels according to respective functionalities in each level. As opposed to local control, which relies only on local measurements, some line of communication between units needs to be made available in order to achieve the coordinated control. Depending on the communication method, three basic coordinated control strategies can be distinguished, i.e., decentralized, centralized, and distributed control. Decentralized control can be regarded as an extension of the local control since it is also based exclusively on local measurements. In contrast, centralized and distributed control strategies rely on digital communication technologies. A number of approaches using these three coordinated control strategies to achieve various control objectives are reviewed in this paper. Moreover, properties of dc MG dynamics and stability are discussed. This paper illustrates that tightly regulated point-of-load converters tend to reduce the stability margins of the system since they introduce negative impedances, which can potentially oscillate with lightly damped power supply input filters. It is also demonstrated that how the stability of the whole system is defined by the relationship of the source and load impedances, referred to as the minor loop gain. Several prominent specifications for the minor loop gain are reviewed. Finally, a number of active stabilization techniques are presented.

  9. "Isogaba Maware": quality control of genome DNA by checkpoints.

    Science.gov (United States)

    Kitazono, A; Matsumoto, T

    1998-05-01

    Checkpoints maintain the interdependency of cell cycle events by permitting the onset of an event only after the completion of the preceding event. The DNA replication checkpoint induces a cell cycle arrest until the completion of the DNA replication. Similarly, the DNA damage checkpoint arrests cell cycle progression if DNA repair is incomplete. A number of genes that play a role in the two checkpoints have been identified through genetic studies in yeasts, and their homologues have been found in fly, mouse, and human. They form signaling cascades activated by a DNA replication block or DNA damage and subsequently generate the negative constraints on cell cycle regulators. The failure of these signaling cascades results in producing offspring that carry mutations or that lack a portion of the genome. In humans, defects in the checkpoints are often associated with cancer-prone diseases. Focusing mainly on the studies in budding and fission yeasts, we summarize the recent progress.

  10. Biological stability of drinking water : Controlling factors, methods, and challenges

    NARCIS (Netherlands)

    Prest, E.I.E.D.; Hammes, F.; Van Loosdrecht, M.C.M.; Vrouwenvelder, J.S.

    2016-01-01

    Biological stability of drinking water refers to the concept of providing consumers with drinking water of same microbial quality at the tap as produced at the water treatment facility. However, uncontrolled growth of bacteria can occur during distribution in water mains and premise plumbing, and

  11. NL(q) Theory: A Neural Control Framework with Global Asymptotic Stability Criteria.

    Science.gov (United States)

    Vandewalle, Joos; De Moor, Bart L.R.; Suykens, Johan A.K.

    1997-06-01

    In this paper a framework for model-based neural control design is presented, consisting of nonlinear state space models and controllers, parametrized by multilayer feedforward neural networks. The models and closed-loop systems are transformed into so-called NL(q) system form. NL(q) systems represent a large class of nonlinear dynamical systems consisting of q layers with alternating linear and static nonlinear operators that satisfy a sector condition. For such NL(q)s sufficient conditions for global asymptotic stability, input/output stability (dissipativity with finite L(2)-gain) and robust stability and performance are presented. The stability criteria are expressed as linear matrix inequalities. In the analysis problem it is shown how stability of a given controller can be checked. In the synthesis problem two methods for neural control design are discussed. In the first method Narendra's dynamic backpropagation for tracking on a set of specific reference inputs is modified with an NL(q) stability constraint in order to ensure, e.g., closed-loop stability. In a second method control design is done without tracking on specific reference inputs, but based on the input/output stability criteria itself, within a standard plant framework as this is done, for example, in H( infinity ) control theory and &mgr; theory. Copyright 1997 Elsevier Science Ltd.

  12. Design of rapid prototype of UAV line-of-sight stabilized control system

    Science.gov (United States)

    Huang, Gang; Zhao, Liting; Li, Yinlong; Yu, Fei; Lin, Zhe

    2018-01-01

    The line-of-sight (LOS) stable platform is the most important technology of UAV (unmanned aerial vehicle), which can reduce the effect to imaging quality from vibration and maneuvering of the aircraft. According to the requirement of LOS stability system (inertial and optical-mechanical combined method) and UAV's structure, a rapid prototype is designed using based on industrial computer using Peripheral Component Interconnect (PCI) and Windows RTX to exchange information. The paper shows the control structure, and circuit system including the inertial stability control circuit with gyro and voice coil motor driven circuit, the optical-mechanical stability control circuit with fast-steering-mirror (FSM) driven circuit and image-deviation-obtained system, outer frame rotary follower, and information-exchange system on PC. Test results show the stability accuracy reaches 5μrad, and prove the effectiveness of the combined line-of-sight stabilization control system, and the real-time rapid prototype runs stable.

  13. Comparative genomic reconstruction of transcriptional networks controlling central metabolism in the Shewanella genus

    Directory of Open Access Journals (Sweden)

    Kovaleva Galina

    2011-06-01

    Full Text Available Abstract Background Genome-scale prediction of gene regulation and reconstruction of transcriptional regulatory networks in bacteria is one of the critical tasks of modern genomics. The Shewanella genus is comprised of metabolically versatile gamma-proteobacteria, whose lifestyles and natural environments are substantially different from Escherichia coli and other model bacterial species. The comparative genomics approaches and computational identification of regulatory sites are useful for the in silico reconstruction of transcriptional regulatory networks in bacteria. Results To explore conservation and variations in the Shewanella transcriptional networks we analyzed the repertoire of transcription factors and performed genomics-based reconstruction and comparative analysis of regulons in 16 Shewanella genomes. The inferred regulatory network includes 82 transcription factors and their DNA binding sites, 8 riboswitches and 6 translational attenuators. Forty five regulons were newly inferred from the genome context analysis, whereas others were propagated from previously characterized regulons in the Enterobacteria and Pseudomonas spp.. Multiple variations in regulatory strategies between the Shewanella spp. and E. coli include regulon contraction and expansion (as in the case of PdhR, HexR, FadR, numerous cases of recruiting non-orthologous regulators to control equivalent pathways (e.g. PsrA for fatty acid degradation and, conversely, orthologous regulators to control distinct pathways (e.g. TyrR, ArgR, Crp. Conclusions We tentatively defined the first reference collection of ~100 transcriptional regulons in 16 Shewanella genomes. The resulting regulatory network contains ~600 regulated genes per genome that are mostly involved in metabolism of carbohydrates, amino acids, fatty acids, vitamins, metals, and stress responses. Several reconstructed regulons including NagR for N-acetylglucosamine catabolism were experimentally validated in S

  14. Sync in Complex Dynamical Networks: Stability, Evolution, Control, and Application

    OpenAIRE

    Li, Xiang

    2005-01-01

    In the past few years, the discoveries of small-world and scale-free properties of many natural and artificial complex networks have stimulated significant advances in better understanding the relationship between the topology and the collective dynamics of complex networks. This paper reports recent progresses in the literature of synchronization of complex dynamical networks including stability criteria, network synchronizability and uniform synchronous criticality in different topologies, ...

  15. PI Stabilization for Congestion Control of AQM Routers with Tuning Parameter Optimization

    Directory of Open Access Journals (Sweden)

    S. Chebli

    2016-09-01

    Full Text Available In this paper, we consider the problem of stabilizing network using a new proportional- integral (PI based congestion controller in active queue management (AQM router; with appropriate model approximation in the first order delay systems, we seek a stability region of the controller by using the Hermite- Biehler theorem, which isapplicable to quasipolynomials. A Genetic Algorithm technique is employed to derive optimal or near optimal PI controller parameters.

  16. Bifurcation and stability of an improved time-delayed fluid flow model in internet congestion control

    International Nuclear Information System (INIS)

    Yu-Liang, Liu; Jie, Zhu; Xiao-Shu, Luo

    2009-01-01

    Based on the fluid flow time-delayed model proposed by Misra et al in internet congestion control, one modified time-delayed model is presented, where the influence of the communication delay on the router queue length is investigated in detail. The main advantage of the new model is that its stability domain is larger even without an extra controller. By linear stability analysis and numerical simulation, the effectiveness and feasibility of the novel model in internet congestion control are verified

  17. Bifurcation and stability of an improved time-delayed fluid flow model in internet congestion control

    Science.gov (United States)

    Liu, Yu-Liang; Zhu, Jie; Luo, Xiao-Shu

    2009-09-01

    Based on the fluid flow time-delayed model proposed by Misra et al in internet congestion control, one modified time-delayed model is presented, where the influence of the communication delay on the router queue length is investigated in detail. The main advantage of the new model is that its stability domain is larger even without an extra controller. By linear stability analysis and numerical simulation, the effectiveness and feasibility of the novel model in internet congestion control are verified.

  18. Stabilization and synchronization of Genesio-Tesi system via single variable feedback controller

    International Nuclear Information System (INIS)

    Wang Guangming

    2010-01-01

    This Letter investigates the stabilization and synchronization of Genesio-Tesi systems. Firstly, modifying the previous method, we stabilize the Genesio-Tesi system. Then, we synchronize two identical Genesio chaotic system by extending the obtained stabilization results. To the best of our knowledge, the above controllers obtained in this Letter are simpler than those obtained in the existing results. Finally, numerical simulations verify the effectiveness and the validity of the above theoretical results.

  19. Mms1 binds to G-rich regions in Saccharomyces cerevisiae and influences replication and genome stability

    NARCIS (Netherlands)

    Wanzek, Katharina; Schwindt, Eike; Capra, John A.; Paeschke, Katrin

    2017-01-01

    The regulation of replication is essential to preserve genome integrity. Mms1 is part of the E3 ubiquitin ligase complex that is linked to replication fork progression. By identifying Mms1 binding sites genome-wide in Saccharomyces cerevisiae we connected Mms1 function to genome integrity and

  20. Study of fuzzy adaptive PID controller on thermal frequency stabilizing laser with double longitudinal modes

    Science.gov (United States)

    Mo, Qingkai; Zhang, Tao; Yan, Yining

    2016-10-01

    There are contradictions among speediness, anti-disturbance performance, and steady-state accuracy caused by traditional PID controller in the existing light source systems of thermal frequency stabilizing laser with double longitudinal modes. In this paper, a new kind of fuzzy adaptive PID controller was designed by combining fuzzy PID control technology and expert system to make frequency stabilizing system obtain the optimal performance. The experiments show that the frequency stability of the designed PID controller is similar to the existing PID controller (the magnitude of frequency stability is less than 10-9 in constant temperature and 10-7 in open air). But the preheating time is shortened obviously (from 10 minutes to 5 minutes) and the anti-disturbance capability is improved significantly (the recovery time needed after strong interference is reduced from 1 minute to 10 seconds).

  1. Investigating the Control of Chlorophyll Degradation by Genomic Correlation Mining.

    Science.gov (United States)

    Ghandchi, Frederick P; Caetano-Anolles, Gustavo; Clough, Steven J; Ort, Donald R

    2016-01-01

    Chlorophyll degradation is an intricate process that is critical in a variety of plant tissues at different times during the plant life cycle. Many of the photoactive chlorophyll degradation intermediates are exceptionally cytotoxic necessitating that the pathway be carefully coordinated and regulated. The primary regulatory step in the chlorophyll degradation pathway involves the enzyme pheophorbide a oxygenase (PAO), which oxidizes the chlorophyll intermediate pheophorbide a, that is eventually converted to non-fluorescent chlorophyll catabolites. There is evidence that PAO is differentially regulated across different environmental and developmental conditions with both transcriptional and post-transcriptional components, but the involved regulatory elements are uncertain or unknown. We hypothesized that transcription factors modulate PAO expression across different environmental conditions, such as cold and drought, as well as during developmental transitions to leaf senescence and maturation of green seeds. To test these hypotheses, several sets of Arabidopsis genomic and bioinformatic experiments were investigated and re-analyzed using computational approaches. PAO expression was compared across varied environmental conditions in the three separate datasets using regression modeling and correlation mining to identify gene elements co-expressed with PAO. Their functions were investigated as candidate upstream transcription factors or other regulatory elements that may regulate PAO expression. PAO transcript expression was found to be significantly up-regulated in warm conditions, during leaf senescence, and in drought conditions, and in all three conditions significantly positively correlated with expression of transcription factor Arabidopsis thaliana activating factor 1 (ATAF1), suggesting that ATAF1 is triggered in the plant response to these processes or abiotic stresses and in result up-regulates PAO expression. The proposed regulatory network includes the

  2. Impacts of P-f & Q-V Droop Control on MicroGrids Transient Stability

    Science.gov (United States)

    Zhao-xia, Xiao; Hong-wei, Fang

    Impacts of P-f & Q-V droop control on MicroGrid transient stability was investigated with a wind unit of asynchronous generator in the MicroGrid. The system frequency stability was explored when the motor load starts and its load power changes, and faults of different types and different locations occurs. The simulations were done by PSCAD/EMTDC.

  3. The effectiveness of core stabilization exercise in adolescent idiopathic scoliosis: A randomized controlled trial.

    Science.gov (United States)

    Gür, Gözde; Ayhan, Cigdem; Yakut, Yavuz

    2017-06-01

    Core stabilization training is used to improve postural balance in musculoskeletal problems. The purpose of this study was to investigate the effectiveness of stabilization training in adolescent idiopathic scoliosis. A randomized controlled trial, pretest-posttest design. In total, 25 subjects with adolescent idiopathic scoliosis were randomly divided into two groups: stabilization group ( n = 12) and control group ( n = 13). The stabilization group received core stabilization in addition to traditional rehabilitation, and the control group received traditional rehabilitation for 10 weeks. Assessment included Cobb's angle on radiograph, apical vertebral rotation in Adam's test, trunk asymmetry (Posterior Trunk Symmetry Index), cosmetic trunk deformity (Trunk Appearance Perception Scale), and quality of life (Scoliosis Research Society-22 questionnaire). Inter-group comparisons showed significantly greater improvements in the mean change in lumbar apical vertebral rotation degree and the pain domain of Scoliosis Research Society-22 in the stabilization group than those in the control group ( p adolescent idiopathic scoliosis. Clinical relevance Stabilization exercises are more effective in reducing rotation deformity and pain than traditional exercises in the conservative rehabilitation of adolescent idiopathic scoliosis. These improvements suggest that stabilization training should be added to rehabilitation programs in adolescent idiopathic scoliosis.

  4. An analysis of the stability and monotonicity of a kind of control models

    Directory of Open Access Journals (Sweden)

    LU Yifa

    2013-06-01

    Full Text Available The stability and monotonicity of control systems with parameters are considered.By the iterative relationship of the coefficients of characteristic polynomials and the Mathematica software,some sufficient conditions for the monotonicity and stability of systems are given.

  5. The Absolute Stability Analysis in Fuzzy Control Systems with Parametric Uncertainties and Reference Inputs

    Science.gov (United States)

    Wu, Bing-Fei; Ma, Li-Shan; Perng, Jau-Woei

    This study analyzes the absolute stability in P and PD type fuzzy logic control systems with both certain and uncertain linear plants. Stability analysis includes the reference input, actuator gain and interval plant parameters. For certain linear plants, the stability (i.e. the stable equilibriums of error) in P and PD types is analyzed with the Popov or linearization methods under various reference inputs and actuator gains. The steady state errors of fuzzy control systems are also addressed in the parameter plane. The parametric robust Popov criterion for parametric absolute stability based on Lur'e systems is also applied to the stability analysis of P type fuzzy control systems with uncertain plants. The PD type fuzzy logic controller in our approach is a single-input fuzzy logic controller and is transformed into the P type for analysis. In our work, the absolute stability analysis of fuzzy control systems is given with respect to a non-zero reference input and an uncertain linear plant with the parametric robust Popov criterion unlike previous works. Moreover, a fuzzy current controlled RC circuit is designed with PSPICE models. Both numerical and PSPICE simulations are provided to verify the analytical results. Furthermore, the oscillation mechanism in fuzzy control systems is specified with various equilibrium points of view in the simulation example. Finally, the comparisons are also given to show the effectiveness of the analysis method.

  6. Short-term genome evolution of Listeria monocytogenes in a non-controlled environment

    Directory of Open Access Journals (Sweden)

    Ivy Reid A

    2008-11-01

    Full Text Available Abstract Background While increasing data on bacterial evolution in controlled environments are available, our understanding of bacterial genome evolution in natural environments is limited. We thus performed full genome analyses on four Listeria monocytogenes, including human and food isolates from both a 1988 case of sporadic listeriosis and a 2000 listeriosis outbreak, which had been linked to contaminated food from a single processing facility. All four isolates had been shown to have identical subtypes, suggesting that a specific L. monocytogenes strain persisted in this processing plant over at least 12 years. While a genome sequence for the 1988 food isolate has been reported, we sequenced the genomes of the 1988 human isolate as well as a human and a food isolate from the 2000 outbreak to allow for comparative genome analyses. Results The two L. monocytogenes isolates from 1988 and the two isolates from 2000 had highly similar genome backbone sequences with very few single nucleotide (nt polymorphisms (1 – 8 SNPs/isolate; confirmed by re-sequencing. While no genome rearrangements were identified in the backbone genome of the four isolates, a 42 kb prophage inserted in the chromosomal comK gene showed evidence for major genome rearrangements. The human-food isolate pair from each 1988 and 2000 had identical prophage sequence; however, there were significant differences in the prophage sequences between the 1988 and 2000 isolates. Diversification of this prophage appears to have been caused by multiple homologous recombination events or possibly prophage replacement. In addition, only the 2000 human isolate contained a plasmid, suggesting plasmid loss or acquisition events. Surprisingly, besides the polymorphisms found in the comK prophage, a single SNP in the tRNA Thr-4 prophage represents the only SNP that differentiates the 1988 isolates from the 2000 isolates. Conclusion Our data support the hypothesis that the 2000 human listeriosis

  7. Brezzi-Pitkaranta stabilization and a priori error analysis for the Stokes Control

    Directory of Open Access Journals (Sweden)

    Aytekin Cibik

    2016-12-01

    Full Text Available In this study, we consider a Brezzi-Pitkaranta stabilization scheme for the optimal control problem governed by stationary Stokes equation, using a P1-P1 interpolation for velocity and pressure. We express the stabilization as extra terms added to the discrete variational form of the problem.  We first prove the stability of the finite element discretization of the problem. Then, we derive a priori error bounds for each variable and present a numerical example to show the effectiveness of the stabilization clearly.

  8. The tumor suppressor SirT2 regulates cell cycle progression and genome stability by modulating the mitotic deposition of H4K20 methylation

    Science.gov (United States)

    The establishment of the epigenetic mark H4K20me1 (monomethylation of H4K20) by PR-Set7 during G2/M directly impacts S-phase progression and genome stability. However, the mechanisms involved in the regulation of this event are not well understood. Here we show that SirT2 regulates H4K20me1 depositi...

  9. Attitude Stabilization Control of a Quadrotor UAV by Using Backstepping Approach

    Directory of Open Access Journals (Sweden)

    Xing Huo

    2014-01-01

    Full Text Available The modeling and attitude stabilization control problems of a four-rotor vertical takeoff and landing unmanned air vehicle (UAV known as the quadrotor are investigated. The quadrotor’s attitude is represented by the unit quaternion rather than Euler angles to avoid singularity problem. Taking dynamical behavior of motors into consideration and ignoring aerodynamic effect, a nonlinear controller is developed to stabilize the attitude. The control design is accomplished by using backstepping control technique. The proposed control law is based on the compensation for the Coriolis and gyroscope torques. Applying Lyapunov stability analysis proves that the closed-loop attitude system is asymptotic stable. Moreover, the controller can guarantee that all the states of the system are uniformly ultimately bounded in the presence of external disturbance torque. The effectiveness of the proposed control approach is analytically authenticated and also validated via simulation study.

  10. Wide area stability analysis and control of interconnected power systems with HVDC and FACTS devices

    Energy Technology Data Exchange (ETDEWEB)

    Li, Yong

    2012-11-01

    In order to damp low-frequency oscillations and improve the overall stability of large-scale interconnected power systems, this book investigates the wide-area stability analysis and control methods from different perspectives. The flexible and fast control capability of high-voltage (FACTS) is investigated in detail to implement a wide-area measurement based damping control. A sequential and global mixed optimization method is proposed to simultaneously optimize local and wide area damping controllers. A wide-area robust coordination method is presented to coordinate multiple wide-area damping controllers (WADC). A delay-dependent robust design method is also proposed to handle time-varying delays commonly existing in wide-area signal communication. A closed-loop hardware experiment is used to validate the damping performance. The research activities of this book include power system stability analysis and control, wide-area damping control as well as HVDC and FACTS technologies.

  11. Positive polynomials and robust stabilization with fixed-order controllers

    Czech Academy of Sciences Publication Activity Database

    Henrion, Didier; Šebek, M.; Kučera, V.

    2003-01-01

    Roč. 48, č. 7 (2003), s. 1178-1186 ISSN 0018-9286 R&D Projects: GA ČR GA102/02/0709; GA MŠk ME 496 Institutional research plan: CEZ:AV0Z1075907 Keywords : fixed-order control lers * linear matrix inequality * polynomials, robust control Subject RIV: BC - Control Systems Theory Impact factor: 1.896, year: 2003

  12. A Genome-wide multidimensional RNAi screen reveals pathways controlling MHC class II antigen presentation

    NARCIS (Netherlands)

    Paul, Petra; van den Hoorn, Tineke; Jongsma, Marlieke L. M.; Bakker, Mark J.; Hengeveld, Rutger; Janssen, Lennert; Cresswell, Peter; Egan, David A.; van Ham, Marieke; ten Brinke, Anja; Ovaa, Huib; Beijersbergen, Roderick L.; Kuijl, Coenraad; Neefjes, Jacques

    2011-01-01

    MHC class II molecules (MHC-II) present peptides to T helper cells to facilitate immune responses and are strongly linked to autoimmune diseases. To unravel processes controlling MHC-II antigen presentation, we performed a genome-wide flow cytometry-based RNAi screen detecting MHC-II expression and

  13. Detecting altered postural control after cerebral concussion in athletes with normal postural stability

    OpenAIRE

    Cavanaugh, J; Guskiewicz, K; Giuliani, C; Marshall, S; Mercer, V; Stergiou, N

    2005-01-01

    Objective: To determine if approximate entropy (ApEn), a regularity statistic from non-linear dynamics, could detect changes in postural control during quiet standing in athletes with normal postural stability after cerebral concussion.

  14. Co-simulation of heavy truck tire dynamics and electronic stability control systems (phase B).

    Science.gov (United States)

    2010-07-01

    In the past decade, electronic stability controls (ESC) have become increasingly common on vehicles operating in the United States. The acceptance of this technology has progressed to the point where all new passenger vehicles sold in the US are requ...

  15. Stabilization of an Underactuated Surface Vessel Based on Adaptive Sliding Mode and Backstepping Control

    Directory of Open Access Journals (Sweden)

    Fuguang Ding

    2013-01-01

    Full Text Available The paper studied controlling problem of an underactuated surface vessel with unknown interferences. It proved that the control problem of underactuated surface vessel can be transformed into the stabilization analysis of two small subsystems. This controller was designed by backstepping method and adaptive sliding mode, was suitable for solving the problem of the control of higher systems, can keep the system global asymptotic stability, and can inhibit unknown interference, and boundary layer can weaken the buffeting generated by sliding mode. The unknown interference was estimated by adaptive function. Finally, the simulation results are given to demonstrate the effectiveness of the proposed control laws.

  16. DESCRIBING FUNCTION METHOD FOR PI-FUZZY CONTROLLED SYSTEMS STABILITY ANALYSIS

    Directory of Open Access Journals (Sweden)

    Stefan PREITL

    2004-12-01

    Full Text Available The paper proposes a global stability analysis method dedicated to fuzzy control systems containing Mamdani PI-fuzzy controllers with output integration to control SISO linear / linearized plants. The method is expressed in terms of relatively simple steps, and it is based on: the generalization of the describing function method for the considered fuzzy control systems to the MIMO case, the approximation of the describing functions by applying the least squares method. The method is applied to the stability analysis of a class of PI-fuzzy controlled servo-systems, and validated by considering a case study.

  17. Robust Stabilization of Fractional-Order Systems with Interval Uncertainties via Fractional-Order Controllers

    Directory of Open Access Journals (Sweden)

    Mohammadtaghi Hamidi Beheshti

    2010-01-01

    Full Text Available We propose a fractional-order controller to stabilize unstable fractional-order open-loop systems with interval uncertainty whereas one does not need to change the poles of the closed-loop system in the proposed method. For this, we will use the robust stability theory of Fractional-Order Linear Time Invariant (FO-LTI systems. To determine the control parameters, one needs only a little knowledge about the plant and therefore, the proposed controller is a suitable choice in the control of interval nonlinear systems and especially in fractional-order chaotic systems. Finally numerical simulations are presented to show the effectiveness of the proposed controller.

  18. Stabilization of prescribed values and periodic orbits with regular and pulse target oriented control

    International Nuclear Information System (INIS)

    Braverman, E.; Chan, B.

    2014-01-01

    Investigating a method of chaos control for one-dimensional maps, where the intervention is proportional to the difference between a fixed value and a current state, we demonstrate that stabilization is possible in one of the two following cases: (1) for small values, the map is increasing and the slope of the line connecting the points on the line with the origin is decreasing; (2) the chaotic map is locally Lipschitz. Moreover, in the latter case we prove that any point of the map can be stabilized. In addition, we study pulse stabilization when the intervention occurs each m-th step and illustrate that stabilization is possible for the first type of maps. In the context of population dynamics, we notice that control with a positive target, even if stabilization is not achieved, leads to persistent solutions and prevents extinction in models which experience the Allee effect

  19. Controllability and Stabilization of Bilinear and Semilinear Partial Differential Equations

    DEFF Research Database (Denmark)

    Krishnaswamy, Vijayaraghavan

    The topic of the thesis is the investigation of the question of controllability of weakly nonlinear partial differntial equations. The method is based on the Hilbert Uniqueness Method.......The topic of the thesis is the investigation of the question of controllability of weakly nonlinear partial differntial equations. The method is based on the Hilbert Uniqueness Method....

  20. The Stability Analysis and New Torque Control Strategy of Direct-Driven PMSG Wind Turbines

    OpenAIRE

    Jun Liu; Feihang Zhou; Gungyi Wang

    2016-01-01

    This paper expounds on the direct-driven PMSG wind power system control strategy, and analyses the stability conditions of the system. The direct-driven PMSG wind power system may generate the intense mechanical vibration, when wind speed changes dramatically. This paper proposes a new type of torque control strategy, which increases the system damping effectively, mitigates mechanical vibration of the system, and enhances the stability conditions of the system. The simulation results verify ...

  1. ANALYSIS OF PERTURBED MOTION STABILITY OF WHEELER VEHICLES BRAKES CONTROL SYSTEM

    Directory of Open Access Journals (Sweden)

    V. Verbytskiyi

    2011-01-01

    Full Text Available The analysis of the perturbed motion stability of the brake automatic control system on the basis of Lyapunov’s second method is carried out. Using transformations of Lurie there has been ob-tained the canonical form of the system of equations of automatic control. It allowed determining the necessary and sufficient conditions of the asymptotic stability of the system irrespective of its initial condition and a definite choice of the admissible characteristic of the regulator.

  2. Open versus Controlled-Access Data | Office of Cancer Genomics

    Science.gov (United States)

    OCG employs stringent human subjects’ protection and data access policies to protect the privacy and confidentiality of the research participants. Depending on the risk of patient identification, OCG programs data are available to the scientific community in two tiers: open or controlled access. Both types of data can be accessed through its corresponding OCG program-specific data matrix or portal. Open-access Data

  3. Comparison of model reference and map based control method for vehicle stability enhancement

    NARCIS (Netherlands)

    Baek, S.; Son, M.; Song, J.; Boo, K.; Kim, H.

    2012-01-01

    A map based controller method to improve a vehicle lateral stability is proposed in this study and compared with the conventional method, a model referenced controller. A model referenced controller to determine compensated yaw moment uses the sliding mode method, but the proposed map based

  4. Cryo-electron Microscopy Reconstruction and Stability Studies of the Wild Type and the R432A Variant of Adeno-associated Virus Type 2 Reveal that Capsid Structural Stability Is a Major Factor in Genome Packaging.

    Science.gov (United States)

    Drouin, Lauren M; Lins, Bridget; Janssen, Maria; Bennett, Antonette; Chipman, Paul; McKenna, Robert; Chen, Weijun; Muzyczka, Nicholas; Cardone, Giovanni; Baker, Timothy S; Agbandje-McKenna, Mavis

    2016-10-01

    The adeno-associated viruses (AAV) are promising therapeutic gene delivery vectors and better understanding of their capsid assembly and genome packaging mechanism is needed for improved vector production. Empty AAV capsids assemble in the nucleus prior to genome packaging by virally encoded Rep proteins. To elucidate the capsid determinants of this process, structural differences between wild-type (wt) AAV2 and a packaging deficient variant, AAV2-R432A, were examined using cryo-electron microscopy and three-dimensional image reconstruction both at an ∼5.0-Å resolution (medium) and also at 3.8- and 3.7-Å resolutions (high), respectively. The high resolution structures showed that removal of the arginine side chain in AAV2-R432A eliminated hydrogen bonding interactions, resulting in altered intramolecular and intermolecular interactions propagated from under the 3-fold axis toward the 5-fold channel. Consistent with these observations, differential scanning calorimetry showed an ∼10°C decrease in thermal stability for AAV2-R432A compared to wt-AAV2. In addition, the medium resolution structures revealed differences in the juxtaposition of the less ordered, N-terminal region of their capsid proteins, VP1/2/3. A structural rearrangement in AAV2-R432A repositioned the βA strand region under the icosahedral 2-fold axis rather than antiparallel to the βB strand, eliminating many intramolecular interactions. Thus, a single amino acid substitution can significantly alter the AAV capsid integrity to the extent of reducing its stability and possibly rendering it unable to tolerate the stress of genome packaging. Furthermore, the data show that the 2-, 3-, and 5-fold regions of the capsid contributed to producing the packaging defect and highlight a tight connection between the entire capsid in maintaining packaging efficiency. The mechanism of AAV genome packaging is still poorly understood, particularly with respect to the capsid determinants of the required capsid

  5. A Combination of PD Controller and PIAFC for Stabilization of “x” Configuration Quadcopter

    Directory of Open Access Journals (Sweden)

    Ni’am Tamami

    2014-06-01

    Full Text Available This paper presents a stabilization control method for “x” configuration quadcopter. The control method used the combination of PD (Proportional Derivative controller and PIAFC (Proportional Integral Active Force Control. PD is used to stabilize quadcopter, and PIAFC is used to reject uncertainty disturbance (e.g. wind by estimating disturbance torque value of quadcopter. The PD with PIAFC provided better result where PIAFC could minimize uncertain disturbance effect. The simulation has successfully give comparation about controller performance (PD, PD-AFC, PD-PIAFC by calculate RMS (Root Mean Square value. PD with AFC gives better result than PD. AFC optimization using PI (PD-PIAFC give best result if compared with PD or PD-AFC. PD-PIAFC has lowest RMS value of result control signal, 0.0389 for constant disturbance and 0.1008 for fluctuated disturbance. Keywords:“x” configuration quadcopter, stability, PD, PIAFC.

  6. Robust stabilization control based on guardian maps theory for a longitudinal model of hypersonic vehicle.

    Science.gov (United States)

    Liu, Yanbin; Liu, Mengying; Sun, Peihua

    2014-01-01

    A typical model of hypersonic vehicle has the complicated dynamics such as the unstable states, the nonminimum phases, and the strong coupling input-output relations. As a result, designing a robust stabilization controller is essential to implement the anticipated tasks. This paper presents a robust stabilization controller based on the guardian maps theory for hypersonic vehicle. First, the guardian maps theories are provided to explain the constraint relations between the open subsets of complex plane and the eigenvalues of the state matrix of closed-loop control system. Then, a general control structure in relation to the guardian maps theories is proposed to achieve the respected design demands. Furthermore, the robust stabilization control law depending on the given general control structure is designed for the longitudinal model of hypersonic vehicle. Finally, a simulation example is provided to verify the effectiveness of the proposed methods.

  7. Assessing control of postural stability in community-living older adults using performance-based limits of stability

    Directory of Open Access Journals (Sweden)

    Boissy Patrick

    2008-03-01

    Full Text Available Abstract Background Balance disability measurements routinely used to identify fall risks in frail populations have limited value in the early detection of postural stability deficits in community-living older adults. The objectives of the study were to 1 measure performance-based limits of stability (LOS in community-living older adults and compare them to theoretical LOS computed from data proposed by the Balance Master® system, 2 explore the feasibility of a new measurement approach based on the assessment of postural stability during weight-shifting tasks at performance-based LOS, 3 quantify intra-session performance variability during multiple trials using the performance-based LOS paradigm. Methods Twenty-four healthy community-living older adults (10 men, 14 women aged between 62 to 85 (mean age ± sd, 71.5 ± 6 yrs participated in the study. Subjects' performance-based LOS were established by asking them to transfer their body weight as far as possible in three directions (forward, right and left without changing their base of support. LOS were computed as the maximal excursion of the COP in each direction among three trials. Participants then performed two experimental tasks that consisted in controlling, with the assistance of visual feedback, their centre of pressure (COP within two predefined targets set at 100% of their performance-based LOS. For each tasks 8 trials were performed. Ground reaction forces and torques during performance-based LOS evaluation and experimental tasks were recorded with a force plate. Sway area and medio-lateral mean COP displacement speed variables were extracted from force plate recordings. Results Significant differences between theoretical LOS computed from maximum leaning angles derived from anthropometric characteristics and performance-based LOS were observed. Results showed that a motor learning effect was present as the participants optimized their weight-shifting strategy through the first three

  8. Stabilization and Control Models of Systems With Hysteresis Nonlinearities

    Directory of Open Access Journals (Sweden)

    Mihail E. Semenov

    2012-05-01

    Full Text Available Mechanical and economic systems with hysteresis nonlinearities are studied in article. Dissipativity condition of inverted pendulum under the hysteresis control is obtained. The solution of the optimal production strategy problem was found where price has hysteresis behaviour.

  9. Controller synthesis for string stability of vehicle platoons

    NARCIS (Netherlands)

    Ploeg, J.; Shukla, D.P.; Wouw, N. van de; Nijmeijer, H.

    2014-01-01

    Cooperative adaptive cruise control (CACC) allows for short-distance automatic vehicle following using intervehicle wireless communication in addition to onboard sensors, thereby potentially improving road throughput. In order to fulfill performance, safety, and comfort requirements, a CACC-equipped

  10. CRISPR-Cas9-Mediated Genome Editing and Transcriptional Control in Yarrowia lipolytica.

    Science.gov (United States)

    Schwartz, Cory; Wheeldon, Ian

    2018-01-01

    The discovery and adaptation of RNA-guided nucleases has resulted in the rapid development of efficient, scalable, and easily accessible synthetic biology tools for targeted genome editing and transcriptional control. In these systems, for example CRISPR-Cas9 from Streptococcus pyogenes, a protein with nuclease activity is targeted to a specific nucleotide sequence by a short RNA molecule, whereupon binding it cleaves the targeted nucleotide strand. To extend this genome-editing ability to the industrially important oleaginous yeast Yarrowia lipolytica, we developed a set of easily usable and effective CRISPR-Cas9 episomal vectors. In this protocols chapter, we first present a method by which arbitrary protein-coding genes can be disrupted via indel formation after CRISPR-Cas9 targeting. A second method demonstrates how the same CRISPR-Cas9 system can be used to induce markerless gene cassette integration into the genome by inducing homologous recombination after DNA cleavage by Cas9. Finally, we describe how a catalytically inactive form of Cas9 fused to a transcriptional repressor can be used to control transcription of native genes in Y. lipolytica. The CRISPR-Cas9 tools and strategies described here greatly increase the types of genome editing and transcriptional control that can be achieved in Y. lipolytica, and promise to facilitate more advanced engineering of this important oleaginous host.

  11. Large Signal Stabilization of Hybrid AC/DC Micro-Grids Using Nonlinear Robust Controller

    Directory of Open Access Journals (Sweden)

    Reza Pejmanfar

    2017-12-01

    Full Text Available This paper presents a robust nonlinear integrated controller to improve stability of hybrid AC/DC micro-grids under islanding mode. The proposed controller includes two independent controllers where each one is responsible to control one part of the system. First controller will improve the stability of input DC/DC converter. Using this controller, the voltage of DC bus is fully stabilized such that when a large disturbance occurs, its voltage will become constant without any significant dynamic. The necessity of DC bus regulation which has not been considered in previous studies, is imminent as it not only improves voltage stability of the micro-grid but also protects consumers which are directly connected to the DC bus, against voltage variations. Frequency stability of the micro-grid is provided by the second proposed controller which is applied to output DC/AC converter of the micro-grid. Adaptive method is used to make the controllers proposed in this paper, robust. Duty cycle of converters switches are adjusted such that voltage and frequency of the micro-grid are set on the desired value in minimum possible time under transient disturbances and uncertainty of the loads as well as micro-sources characteristics.

  12. Virtual Inertia Control-Based Model Predictive Control for Microgrid Frequency Stabilization Considering High Renewable Energy Integration

    Directory of Open Access Journals (Sweden)

    Thongchart Kerdphol

    2017-05-01

    Full Text Available Renewable energy sources (RESs, such as wind and solar generations, equip inverters to connect to the microgrids. These inverters do not have any rotating mass, thus lowering the overall system inertia. This low system inertia issue could affect the microgrid stability and resiliency in the situation of uncertainties. Today’s microgrids will become unstable if the capacity of RESs become larger and larger, leading to the weakening of microgrid stability and resilience. This paper addresses a new concept of a microgrid control incorporating a virtual inertia system based on the model predictive control (MPC to emulate virtual inertia into the microgrid control loop, thus stabilizing microgrid frequency during high penetration of RESs. The additional controller of virtual inertia is applied to the microgrid, employing MPC with virtual inertia response. System modeling and simulations are carried out using MATLAB/Simulink® software. The simulation results confirm the superior robustness and frequency stabilization effect of the proposed MPC-based virtual inertia control in comparison to the fuzzy logic system and conventional virtual inertia control in a system with high integration of RESs. The proposed MPC-based virtual inertia control is able to improve the robustness and frequency stabilization of the microgrid effectively.

  13. The Quiescent Cellular State is Arf/p53-Dependent and Associated with H2AX Downregulation and Genome Stability

    Directory of Open Access Journals (Sweden)

    Mitsuko Masutani

    2012-05-01

    Full Text Available Cancer is a disease associated with genomic instability and mutations. Excluding some tumors with specific chromosomal translocations, most cancers that develop at an advanced age are characterized by either chromosomal or microsatellite instability. However, it is still unclear how genomic instability and mutations are generated during the process of cellular transformation and how the development of genomic instability contributes to cellular transformation. Recent studies of cellular regulation and tetraploidy development have provided insights into the factors triggering cellular transformation and the regulatory mechanisms that protect chromosomes from genomic instability.

  14. Feedback-controlled NTM stabilization on ASDEX Upgrade

    Directory of Open Access Journals (Sweden)

    Stober J.

    2015-01-01

    Full Text Available On ASDEX Upgrade a concept for real-time stabilization of NTMs has been realized and successfully applied to (3,2- and (2,1-NTMs. Since most of the work has meanwhile been published elsewhere, a short summary with the appropriate references is given. Limitations, deficits and future extensions of the system are discussed. In a second part the recent work on using modulated ECCD for NTM stabilisation is described in some detail. In these experiments ECCD power is modulated according to a magnetic footprint of the rotating NTM. In agreement with earlier results it could be shown that O-point heating reduces the necessary average power for stabilisation whereas X-point heating hampers stabilisation. Although this modulated scheme is not relevant for routine NTM stabilisation on ASDEX Upgrade it may be mandatory for ITER or DEMO. On ASDEX Upgrade it has been re-developed to demonstrate the usage of a FAst DIrectional Switch to continously heat the O-point of the rotating island with only one gyrotron switching between two launchers which target the mode at locations separated in phase by 180 degrees as described in [1].

  15. Robust D-Stability Controller Design for a Ducted Fan Unmanned Aerial Vehicle

    Directory of Open Access Journals (Sweden)

    Xiao-lu Ren

    2014-01-01

    Full Text Available This paper deals with the aerodynamic modeling of a small ducted fan UAV and the problem of attitude stabilization when the parameter of the vehicle is varied. The main aerodynamic model of the hovering flight UAV is first presented. Then, an attitude control is designed from a linearization of the dynamic model around the hovering flight, which is based on the H∞ output feedback control theory with D-stability. Simulation results show that such method has good robustness to the attitude system. They can meet the requirements of attitude control and verify further the feasibility of such a control strategy.

  16. Electric Machine with Boosted Inductance to Stabilize Current Control

    Science.gov (United States)

    Abel, Steve

    2013-01-01

    High-powered motors typically have very low resistance and inductance (R and L) in their windings. This makes the pulse-width modulated (PWM) control of the current very difficult, especially when the bus voltage (V) is high. These R and L values are dictated by the motor size, torque (Kt), and back-emf (Kb) constants. These constants are in turn set by the voltage and the actuation torque-speed requirements. This problem is often addressed by placing inductive chokes within the controller. This approach is undesirable in that space is taken and heat is added to the controller. By keeping the same motor frame, reducing the wire size, and placing a correspondingly larger number of turns in each slot, the resistance, inductance, torque constant, and back-emf constant are all increased. The increased inductance aids the current control but ruins the Kt and Kb selections. If, however, a fraction of the turns is moved from their "correct slot" to an "incorrect slot," the increased R and L values are retained, but the Kt and Kb values are restored to the desired values. This approach assumes that increased resistance is acceptable to a degree. In effect, the heat allocated to the added inductance has been moved from the controller to the motor body, which in some cases is preferred.

  17. Stability and control of wind farms in power systems

    DEFF Research Database (Denmark)

    Jauch, Clemens

    is part of the project. The mostextensive modelling work deals with the design of the electrical part of the variable speed turbine and its controls. To simulate realistic grid operation the wind turbine models are connected to an aggregated model of the Nordic power system. For thatpurpose the Nordic...... through transient faults. With these transient fault controllers the wind turbines can stay connected to the grid, such that their generation capacity is sustained, and normal gridoperation can resume, after the fault is cleared. Transient faults in the transmission system often cause power system...... oscillations. To further support the grid, a situation is assumed, where in future, wind turbines will be required to contribute to thedamping of these power system oscillations. Power system oscillations are counteracted with a controlled injection of oscillating active power. With an active-stall turbine...

  18. Nonlinear Slewing Spacecraft Control Based on Exergy, Power Flow, and Static and Dynamic Stability

    Science.gov (United States)

    Robinett, Rush D.; Wilson, David G.

    2009-10-01

    This paper presents a new nonlinear control methodology for slewing spacecraft, which provides both necessary and sufficient conditions for stability by identifying the stability boundaries, rigid body modes, and limit cycles. Conservative Hamiltonian system concepts, which are equivalent to static stability of airplanes, are used to find and deal with the static stability boundaries: rigid body modes. The application of exergy and entropy thermodynamic concepts to the work-rate principle provides a natural partitioning through the second law of thermodynamics of power flows into exergy generator, dissipator, and storage for Hamiltonian systems that is employed to find the dynamic stability boundaries: limit cycles. This partitioning process enables the control system designer to directly evaluate and enhance the stability and performance of the system by balancing the power flowing into versus the power dissipated within the system subject to the Hamiltonian surface (power storage). Relationships are developed between exergy, power flow, static and dynamic stability, and Lyapunov analysis. The methodology is demonstrated with two illustrative examples: (1) a nonlinear oscillator with sinusoidal damping and (2) a multi-input-multi-output three-axis slewing spacecraft that employs proportional-integral-derivative tracking control with numerical simulation results.

  19. Single Stance Stability and Proprioceptive Control in Older Adults Living at Home: Gender and Age Differences

    Directory of Open Access Journals (Sweden)

    Dario Riva

    2013-01-01

    Full Text Available In developed countries, falls in older people represent a rising problem. As effective prevention should start before the risk becomes evident, an early predictor is needed. Single stance instability would appear as a major risk factor. Aims of the study were to describe single stance stability, its sensory components, and their correlation with age and gender. A random sample of 597 older adults (319 men, 278 women living at home, aged 65–84, was studied. Stability tests were performed with an electronic postural station. The single stance test showed the impairment of single stance stability in older individuals (75–84 yrs. The significant decline of stability in the older subjects may be explained by the impairment of proprioceptive control together with the decrease in compensatory visual stabilization and emergency responses. Younger subjects (65–74 yrs exhibited better, but still inadequate, proprioceptive control with compensatory visual stabilization. Gender differences appeared in older subjects: women were significantly less stable than men. The measurement of the sensory components of single stance stability could aid in the early detection of a decay in antigravity movements many years before the risk of falling becomes evident. Adequate proprioceptive control could mitigate the effects of all other risks of falling.

  20. Single Stance Stability and Proprioceptive Control in Older Adults Living at Home: Gender and Age Differences

    Science.gov (United States)

    Riva, Dario; Mamo, Carlo; Fanì, Mara; Saccavino, Patrizia; Rocca, Flavio; Momenté, Manuel; Fratta, Marianna

    2013-01-01

    In developed countries, falls in older people represent a rising problem. As effective prevention should start before the risk becomes evident, an early predictor is needed. Single stance instability would appear as a major risk factor. Aims of the study were to describe single stance stability, its sensory components, and their correlation with age and gender. A random sample of 597 older adults (319 men, 278 women) living at home, aged 65–84, was studied. Stability tests were performed with an electronic postural station. The single stance test showed the impairment of single stance stability in older individuals (75–84 yrs). The significant decline of stability in the older subjects may be explained by the impairment of proprioceptive control together with the decrease in compensatory visual stabilization and emergency responses. Younger subjects (65–74 yrs) exhibited better, but still inadequate, proprioceptive control with compensatory visual stabilization. Gender differences appeared in older subjects: women were significantly less stable than men. The measurement of the sensory components of single stance stability could aid in the early detection of a decay in antigravity movements many years before the risk of falling becomes evident. Adequate proprioceptive control could mitigate the effects of all other risks of falling. PMID:23984068

  1. Analysis and improvement of digital control stability for master-slave manipulator system

    International Nuclear Information System (INIS)

    Yoshida, Koichi; Yabuta, Tetsuro

    1992-01-01

    Some bilateral controls of master-slave system have been designed, which can realize high-fidelity telemanipulation as if the operator were manipulating the object directly. While usual robot systems are controlled by software-servo system using digital computer, little work has been published on design and analysis for digital control of these systems, which must consider time-delay of sensor signals and zero order hold effect of command signals on actuators. This paper presents a digital control analysis for single degree of freedom master-slave system including impedance models of both the human operator and the task object, which clarifies some index for the stability. The stability result shows a virtual master-slave system concepts, which improve the digital control stability. We first analyze a dynamic control method of master-slave system in discrete-time system for the stability problem, which can realize high-fidelity telemanipulation in the continuous-time. Secondly, using the results of the stability analysis, the robust control scheme for master-slave system is proposed, and the validity of this scheme is finally confirmed by the simulation. Consequently, it would be considered that any combination of master and slave modules with dynamic model of these manipulators is possible to construct the stable master-slave system. (author)

  2. Genome-wide association identifies multiple genomic regions associated with susceptibility to and control of ovine lentivirus.

    Directory of Open Access Journals (Sweden)

    Stephen N White

    Full Text Available BACKGROUND: Like human immunodeficiency virus (HIV, ovine lentivirus (OvLV is macrophage-tropic and causes lifelong infection. OvLV infects one quarter of U.S. sheep and induces pneumonia and body condition wasting. There is no vaccine to prevent OvLV infection and no cost-effective treatment for infected animals. However, breed differences in prevalence and proviral concentration have indicated a genetic basis for susceptibility to OvLV. A recent study identified TMEM154 variants in OvLV susceptibility. The objective here was to identify additional loci associated with odds and/or control of OvLV infection. METHODOLOGY/PRINCIPAL FINDINGS: This genome-wide association study (GWAS included 964 sheep from Rambouillet, Polypay, and Columbia breeds with serological status and proviral concentration phenotypes. Analytic models accounted for breed and age, as well as genotype. This approach identified TMEM154 (nominal P=9.2×10(-7; empirical P=0.13, provided 12 additional genomic regions associated with odds of infection, and provided 13 regions associated with control of infection (all nominal P<1 × 10(-5. Rapid decline of linkage disequilibrium with distance suggested many regions included few genes each. Genes in regions associated with odds of infection included DPPA2/DPPA4 (empirical P=0.006, and SYTL3 (P=0.051. Genes in regions associated with control of infection included a zinc finger cluster (ZNF192, ZSCAN16, ZNF389, and ZNF165; P=0.001, C19orf42/TMEM38A (P=0.047, and DLGAP1 (P=0.092. CONCLUSIONS/SIGNIFICANCE: These associations provide targets for mutation discovery in sheep susceptibility to OvLV. Aside from TMEM154, these genes have not been associated previously with lentiviral infection in any species, to our knowledge. Further, data from other species suggest functional hypotheses for future testing of these genes in OvLV and other lentiviral infections. Specifically, SYTL3 binds and may regulate RAB27A, which is required for enveloped

  3. Fault Tolerant Emergency Control to Preserve Power System Stability

    DEFF Research Database (Denmark)

    Pedersen, Andreas Søndergaard; Richter, Jan H.; Tabatabaeipour, Mojtaba

    2016-01-01

    This paper introduces a method for fault-masking and system reconfiguration in power transmission systems. The paper demonstrates how faults are handled by reconfiguring remaining controls through utilisation of wide-area measurement in real time. It is shown how reconfiguration can be obtained u...

  4. Maintaining genetic stability in a control flock of South African ...

    African Journals Online (AJOL)

    means of whole progeny and replacement groups for the measured characters ... The whole problem of the estimation of genetic change was ... that a genetic control flock is a segregating population in which ... 5,5 years, were divided into five equal groups by stratified ..... A note on tests of significance and optimal ex-.

  5. Comparative genomics of metabolic capacities of regulons controlled by cis-regulatory RNA motifs in bacteria.

    Science.gov (United States)

    Sun, Eric I; Leyn, Semen A; Kazanov, Marat D; Saier, Milton H; Novichkov, Pavel S; Rodionov, Dmitry A

    2013-09-02

    In silico comparative genomics approaches have been efficiently used for functional prediction and reconstruction of metabolic and regulatory networks. Riboswitches are metabolite-sensing structures often found in bacterial mRNA leaders controlling gene expression on transcriptional or translational levels.An increasing number of riboswitches and other cis-regulatory RNAs have been recently classified into numerous RNA families in the Rfam database. High conservation of these RNA motifs provides a unique advantage for their genomic identification and comparative analysis. A comparative genomics approach implemented in the RegPredict tool was used for reconstruction and functional annotation of regulons controlled by RNAs from 43 Rfam families in diverse taxonomic groups of Bacteria. The inferred regulons include ~5200 cis-regulatory RNAs and more than 12000 target genes in 255 microbial genomes. All predicted RNA-regulated genes were classified into specific and overall functional categories. Analysis of taxonomic distribution of these categories allowed us to establish major functional preferences for each analyzed cis-regulatory RNA motif family. Overall, most RNA motif regulons showed predictable functional content in accordance with their experimentally established effector ligands. Our results suggest that some RNA motifs (including thiamin pyrophosphate and cobalamin riboswitches that control the cofactor metabolism) are widespread and likely originated from the last common ancestor of all bacteria. However, many more analyzed RNA motifs are restricted to a narrow taxonomic group of bacteria and likely represent more recent evolutionary innovations. The reconstructed regulatory networks for major known RNA motifs substantially expand the existing knowledge of transcriptional regulation in bacteria. The inferred regulons can be used for genetic experiments, functional annotations of genes, metabolic reconstruction and evolutionary analysis. The obtained genome

  6. Microcrystalline silicon deposition: Process stability and process control

    International Nuclear Information System (INIS)

    Donker, M.N. van den; Kilper, T.; Grunsky, D.; Rech, B.; Houben, L.; Kessels, W.M.M.; Sanden, M.C.M. van de

    2007-01-01

    Applying in situ process diagnostics, we identified several process drifts occurring in the parallel plate plasma deposition of microcrystalline silicon (μc-Si:H). These process drifts are powder formation (visible from diminishing dc-bias and changing spatial emission profile on a time scale of 10 0 s), transient SiH 4 depletion (visible from a decreasing SiH emission intensity on a time scale of 10 2 s), plasma heating (visible from an increasing substrate temperature on a time scale of 10 3 s) and a still puzzling long-term drift (visible from a decreasing SiH emission intensity on a time scale of 10 4 s). The effect of these drifts on the crystalline volume fraction in the deposited films is investigated by selected area electron diffraction and depth-profiled Raman spectroscopy. An example shows how the transient depletion and long-term drift can be prevented by suitable process control. Solar cells deposited using this process control show enhanced performance. Options for process control of plasma heating and powder formation are discussed

  7. Control and Stabilization of the Benjamin-Ono Equation in {L^2({{T})}}

    Science.gov (United States)

    Laurent, Camille; Linares, Felipe; Rosier, Lionel

    2015-12-01

    We study the control and stabilization of the Benjamin-Ono equation in {L^2({T})}, the lowest regularity where the initial value problem is well-posed. This problem was already initiated in Linares and Rosier (Trans Am Math Soc 367:4595-4626, 2015) where a stronger stabilization term was used (that makes the equation of parabolic type in the control zone). Here we employ a more natural stabilization term related to the L 2-norm. Moreover, by proving a theorem of controllability in L 2, we manage to prove the global controllability in large time. Our analysis relies strongly on the bilinear estimates proved in Molinet and Pilod (Anal PDE 5:365-395, 2012) and some new extension of these estimates established here.

  8. Decentralized Feedback Controllers for Exponential Stabilization of Hybrid Periodic Orbits: Application to Robotic Walking*

    Science.gov (United States)

    Hamed, Kaveh Akbari; Gregg, Robert D.

    2016-01-01

    This paper presents a systematic algorithm to design time-invariant decentralized feedback controllers to exponentially stabilize periodic orbits for a class of hybrid dynamical systems arising from bipedal walking. The algorithm assumes a class of parameterized and nonlinear decentralized feedback controllers which coordinate lower-dimensional hybrid subsystems based on a common phasing variable. The exponential stabilization problem is translated into an iterative sequence of optimization problems involving bilinear and linear matrix inequalities, which can be easily solved with available software packages. A set of sufficient conditions for the convergence of the iterative algorithm to a stabilizing decentralized feedback control solution is presented. The power of the algorithm is demonstrated by designing a set of local nonlinear controllers that cooperatively produce stable walking for a 3D autonomous biped with 9 degrees of freedom, 3 degrees of underactuation, and a decentralization scheme motivated by amputee locomotion with a transpelvic prosthetic leg. PMID:27990059

  9. Pseudodifferential Perturbations and Stabilization of Distributed Parameter Systems: Dirichlet Feedback Control Problems

    DEFF Research Database (Denmark)

    Pedersen, Michael

    1991-01-01

    The stabilization problems for parabolic and hyperbolic partial differential equations with Dirichlet boundary condition are considered. The systems are stabilized by a boundary feedback in(1) The operator equation,(2) The boundary condition,(3) Both the operator equation and the boundary condition...... turns out to be a shortcut to some of the stabilization results of Lasiecka and Triggiani in [J. Differential Equations, 47 (1983), pp. 245-272], [SIAM J. Control Optim., 21(1983), pp. 766-802], and [Appl. Math. Optim., 8(1981), pp. 1-37], and it illuminates to some extent how a change of boundary...

  10. Stability test and analysis of the Space Shuttle Primary Reaction Control Subsystem thruster

    Science.gov (United States)

    Applewhite, John; Hurlbert, Eric; Krohn, Douglas; Arndt, Scott; Clark, Robert

    1992-01-01

    The results are reported of a test program conducted on the Space Shuttle Primary Reaction Control Subsystem thruster in order to investigate the effects of trapped helium bubbles and saturated propellants on stability, determine if thruster-to-thruster stability variations are significant, and determine stability under STS-representative conditions. It is concluded that the thruster design is highly reliable in flight and that burn-through has not occurred. Significantly unstable thrusters are screened out, and wire wrap is found to protect against chamber burn-throughs and to provide a fail-safe thruster for this situation.

  11. Stabilizing model predictive control of a gantry crane based on flexible set-membership constraints

    NARCIS (Netherlands)

    Iles, Sandor; Lazar, M.; Kolonic, Fetah; Jadranko, Matusko

    2015-01-01

    This paper presents a stabilizing distributed model predictive control of a gantry crane taking into account the variation of cable length. The proposed algorithm is based on the off-line computation of a sequence of 1-step controllable sets and a condition that enables flexible convergence towards

  12. Proportional derivative based stabilizing control of paralleled grid converters with cables in renewable power plants

    DEFF Research Database (Denmark)

    Wang, Xiongfei; Blaabjerg, Frede; Loh, Poh Chiang

    2014-01-01

    consisting in LCL filters and cables. Both grid and converter current controls are analyzed. The frequency region, within which the system may be destabilized, is identified by means of the impedance-based stability analysis and frequency-domain passivity theory. A proportional derivative control strategy...

  13. Robust Stability Clearance of Flight Control Law Based on Global Sensitivity Analysis

    OpenAIRE

    Ou, Liuli; Liu, Lei; Dong, Shuai; Wang, Yongji

    2014-01-01

    To validate the robust stability of the flight control system of hypersonic flight vehicle, which suffers from a large number of parametrical uncertainties, a new clearance framework based on structural singular value ( $\\mu $ ) theory and global uncertainty sensitivity analysis (SA) is proposed. In this framework, SA serves as the preprocess of uncertain model to be analysed to help engineers to determine which uncertainties affect the stability of the closed loop system more slightly. By ig...

  14. Quality control and stability study of 100 mg/ml paracetamol oral drops

    International Nuclear Information System (INIS)

    Garcia Penna, Caridad M.; Montes de Oca Porto, Yanet; Salomon Izquierdo, Suslebys

    2013-01-01

    Paracetamol is an effective analgesic and antipyretic drug of the non-steroidal anti-inflammatory drug group. Paracetamol oral drops are indicated for use in infant population aged up to 5 years to relieve fever, headache, toothache and symptomatic relief of common cold. To validate two analytical methods for the quality control and the stability study and to study the stability of 100 mg/ml Paracetamol oral drops made in Cuba

  15. Electronic differential control of 2WD electric vehicle considering steering stability

    Science.gov (United States)

    Hua, Yiding; Jiang, Haobin; Geng, Guoqing

    2017-03-01

    Aiming at the steering wheel differential steering control technology of rear wheel independent driving electric wheel, considering the assisting effect of electronic differential control on vehicle steering, based on the high speed steering characteristic of electric wheel car, the electronic differential speed of auxiliary wheel steering is also studied. A yaw moment control strategy is applied to the vehicle at high speed. Based on the vehicle stability reference value, yaw rate is used to design the fuzzy controller to distribute the driving wheel torque. The simulation results show that the basic electronic differential speed function is realized based on the yaw moment control strategy, while the vehicle stability control is improved and the driving safety is enhanced. On the other hand, the torque control strategy can also assist steering of vehicle.

  16. Determination of the frequency of polymorphisms in genes related to the genome stability maintenance of the population residing at Monte Alegre, PA (Brazil) municipality

    International Nuclear Information System (INIS)

    Hozumi, Cristiny Gomes

    2010-01-01

    The human exposure to ionizing radiation coming from natural sources is an inherent feature of human life on earth, for man and all living things have always been exposed to these sources. Ionizing radiation is a known genotoxic agent which can affect the genomic stability and genes related to DNA repair may play a role when they have committed certain polymorphism. This study aimed to analyze the frequency of polymorphisms (SNPs) in genes of DNA repair and cell cycle control: hOGG1 (Ser326Cys), XRCC3 (Thr241 Met) and p53 (Arg72Pro) in saliva samples from a population located Monte Alegre, state of Para were collected in August 2008 and 40 samples of men and 46 samples of women, adding a total of 86 samples. By RFLP was determined the frequency of homozygous genotypes and / or heterozygous for polymorphic genes. The I)OGG1 gene was 5% of the allele 326Cys, XRCC3 gene found about 21 % of the allele 241 Met and p53 gene showed 40.8% of the 72Pro allele. And the genotype frequencies of individuals for the three genes were 91.04%, 88.06% and 59.7% for homozygous wild genotype, 5.97%, 11.94% and 22.39% for heterozygote genotype and 2,99%, zero and 17:91% for homozygous polymorphic hOGG1 genes respectively, XRCC3, p53. These values are similar to those found in previous studies. The influence of these polymorphisms, which are involved in DNA repair and consequent genotoxicity induced by radiation depends on dose and exposure factors such as smoking, which is statistically a factor in public health surveillance in the region. This study gathered information and molecular epidemiology in Monte Alegre, that help to characterization of local population. (author)

  17. Whole-Genome Sequence of Pseudomonas graminis Strain UASWS1507, a Potential Biological Control Agent and Biofertilizer Isolated in Switzerland.

    Science.gov (United States)

    Crovadore, Julien; Calmin, Gautier; Chablais, Romain; Cochard, Bastien; Schulz, Torsten; Lefort, François

    2016-10-06

    We report here the whole-genome shotgun sequence of the strain UASWS1507 of the species Pseudomonas graminis, isolated in Switzerland from an apple tree. This is the first genome registered for this species, which is considered as a potential and valuable resource of biological control agents and biofertilizers for agriculture. Copyright © 2016 Crovadore et al.

  18. Robust Frequency and Voltage Stability Control Strategy for Standalone AC/DC Hybrid Microgrid

    Directory of Open Access Journals (Sweden)

    Furqan Asghar

    2017-05-01

    Full Text Available The microgrid (MG concept is attracting considerable attention as a solution to energy deficiencies, especially in remote areas, but the intermittent nature of renewable sources and varying loads cause many control problems and thereby affect the quality of power within a microgrid operating in standalone mode. This might cause large frequency and voltage deviations in the system due to unpredictable output power fluctuations. Furthermore, without any main grid support, it is more complex to control and manage the system. In past, droop control and various other coordination control strategies have been presented to stabilize the microgrid frequency and voltages, but in order to utilize the available resources up to their maximum capacity in a positive way, new and robust control mechanisms are required. In this paper, a standalone microgrid is presented, which integrates renewable energy-based distributed generations and local loads. A fuzzy logic-based intelligent control technique is proposed to maintain the frequency and DC (direct current-link voltage stability for sudden changes in load or generation power. Also from a frequency control perspective, a battery energy storage system (BESS is suggested as a replacement for a synchronous generator to stabilize the nominal system frequency as a synchronous generator is unable to operate at its maximum efficiency while being controlled for stabilization purposes. Likewise, a super capacitor (SC and BESS is used to stabilize DC bus voltages even though maximum possible energy is being extracted from renewable generated sources using maximum power point tracking. This newly proposed control method proves to be effective by reducing transient time, minimizing the frequency deviations, maintaining voltages even though maximum power point tracking is working and preventing generators from exceeding their power ratings during disturbances. However, due to the BESS limited capacity, load switching

  19. Investigation of electrodynamic stabilization and control of long orbiting tethers

    Science.gov (United States)

    Colombo, G.; Arnold, D.

    1984-01-01

    The state-of-the-art in tether modelling among participants in the Tethered Satellite System (TSS) Program, the slack tether and its behavior, and certain advanced applications of the tether to problems in orbital mechanics are identified. The features and applications of the TSS software set are reviewed. Modelling the slack tether analytically with as many as 50 mass points and the application of this new model to a study of the behavior of a broken tether near the Shuttle are described. A reel control algorithm developed by SAO and examples of its use are described, including an example which also demonstrates the use of the tether in transferring a heavy payload from a low-orbiting Shuttle to a high circular orbit. Capture of a low-orbiting payload by a Space Station in high circular orbit is described. Energy transfer within a dumbbell-type spacecraft by cyclical reeling operations or gravitational effects on the natural elasticity of the connecting tether, it is shown, can circularize the orbit of the spacecraft.

  20. Adaptive Neural-Sliding Mode Control of Active Suspension System for Camera Stabilization

    Directory of Open Access Journals (Sweden)

    Feng Zhao

    2015-01-01

    Full Text Available The camera always suffers from image instability on the moving vehicle due to the unintentional vibrations caused by road roughness. This paper presents a novel adaptive neural network based on sliding mode control strategy to stabilize the image captured area of the camera. The purpose is to suppress vertical displacement of sprung mass with the application of active suspension system. Since the active suspension system has nonlinear and time varying characteristics, adaptive neural network (ANN is proposed to make the controller robustness against systematic uncertainties, which release the model-based requirement of the sliding model control, and the weighting matrix is adjusted online according to Lyapunov function. The control system consists of two loops. The outer loop is a position controller designed with sliding mode strategy, while the PID controller in the inner loop is to track the desired force. The closed loop stability and asymptotic convergence performance can be guaranteed on the basis of the Lyapunov stability theory. Finally, the simulation results show that the employed controller effectively suppresses the vibration of the camera and enhances the stabilization of the entire camera, where different excitations are considered to validate the system performance.

  1. Simple Model-Free Controller for the Stabilization of Planetary Inverted Pendulum

    Directory of Open Access Journals (Sweden)

    Huanhuan Mai

    2012-01-01

    Full Text Available A simple model-free controller is presented for solving the nonlinear dynamic control problems. As an example of the problem, a planetary gear-type inverted pendulum (PIP is discussed. To control the inherently unstable system which requires real-time control responses, the design of a smart and simple controller is made necessary. The model-free controller proposed includes a swing-up controller part and a stabilization controller part; neither controller has any information about the PIP. Since the input/output scaling parameters of the fuzzy controller are highly sensitive, we use genetic algorithm (GA to obtain the optimal control parameters. The experimental results show the effectiveness and robustness of the present controller.

  2. Genomic instability following irradiation

    International Nuclear Information System (INIS)

    Hacker-Klom, U.B.; Goehde, W.

    2001-01-01

    Ionising irradiation may induce genomic instability. The broad spectrum of stress reactions in eukaryontic cells to irradiation complicates the discovery of cellular targets and pathways inducing genomic instability. Irradiation may initiate genomic instability by deletion of genes controlling stability, by induction of genes stimulating instability and/or by activating endogeneous cellular viruses. Alternatively or additionally it is discussed that the initiation of genomic instability may be a consequence of radiation or other agents independently of DNA damage implying non nuclear targets, e.g. signal cascades. As a further mechanism possibly involved our own results may suggest radiation-induced changes in chromatin structure. Once initiated the process of genomic instability probably is perpetuated by endogeneous processes necessary for proliferation. Genomic instability may be a cause or a consequence of the neoplastic phenotype. As a conclusion from the data available up to now a new interpretation of low level radiation effects for radiation protection and in radiotherapy appears useful. The detection of the molecular mechanisms of genomic instability will be important in this context and may contribute to a better understanding of phenomenons occurring at low doses <10 cSv which are not well understood up to now. (orig.)

  3. Feedback stabilization of controlled dynamical systems in honor of Laurent Praly

    CERN Document Server

    2017-01-01

    This book is a tribute to Professor Laurent Praly and follows on from a workshop celebrating the occasion of his 60th birthday. It presents new and unified visions of the numerous problems that Laurent Praly has worked on in his prolific career: adaptive control, output feedback and observers, stability and stabilization. His main contributions are the central topic of this book. The book collects contributions written by prominent international experts in the control community, addressing a rich variety of topics: emerging ideas, advanced applications, and theoretical concepts. Organized in three sections, the first section covers the field of adaptive control, where Laurent Praly started his career. The second section focuses on stabilization and output feedback, which is also the topic of the second half of his career. Lastly, the third section presents the emerging research that will form Laurent Praly’s scientific legacy.

  4. Thermodynamic stability of driven open systems and control of phase separation by electro-autocatalysis.

    Science.gov (United States)

    Bazant, Martin Z

    2017-07-01

    Motivated by the possibility of electrochemical control of phase separation, a variational theory of thermodynamic stability is developed for driven reactive mixtures, based on a nonlinear generalization of the Cahn-Hilliard and Allen-Cahn equations. The Glansdorff-Prigogine stability criterion is extended for driving chemical work, based on variations of nonequilibrium Gibbs free energy. Linear stability is generally determined by the competition of chemical diffusion and driven autocatalysis. Novel features arise for electrochemical systems, related to controlled total current (galvanostatic operation), concentration-dependent exchange current (Butler-Volmer kinetics), and negative differential reaction resistance (Marcus kinetics). The theory shows how spinodal decomposition can be controlled by solo-autocatalytic charge transfer, with only a single faradaic reaction. Experimental evidence is presented for intercalation and electrodeposition in rechargeable batteries, and further applications are discussed in solid state ionics, electrovariable optics, electrochemical precipitation, and biological pattern formation.

  5. Improved Polynomial Fuzzy Modeling and Controller with Stability Analysis for Nonlinear Dynamical Systems

    Directory of Open Access Journals (Sweden)

    Hamed Kharrati

    2012-01-01

    Full Text Available This study presents an improved model and controller for nonlinear plants using polynomial fuzzy model-based (FMB systems. To minimize mismatch between the polynomial fuzzy model and nonlinear plant, the suitable parameters of membership functions are determined in a systematic way. Defining an appropriate fitness function and utilizing Taylor series expansion, a genetic algorithm (GA is used to form the shape of membership functions in polynomial forms, which are afterwards used in fuzzy modeling. To validate the model, a controller based on proposed polynomial fuzzy systems is designed and then applied to both original nonlinear plant and fuzzy model for comparison. Additionally, stability analysis for the proposed polynomial FMB control system is investigated employing Lyapunov theory and a sum of squares (SOS approach. Moreover, the form of the membership functions is considered in stability analysis. The SOS-based stability conditions are attained using SOSTOOLS. Simulation results are also given to demonstrate the effectiveness of the proposed method.

  6. Advanced controls for stability assessment of solar dynamics space power generation

    Science.gov (United States)

    Momoh, James A.; Anwah, Nnamdi A.

    1995-01-01

    In support of the power requirements for the Space Station Alpha (SSA), a joint program by the U.S. and Russia for a permanently manned space station to be launched into orbit by 1998, a robust control scheme is needed to assure the stability of the rotating machines that will be integrated into the power subsystem. A framework design and systems studies for modeling and analysis is presented. It employs classical d-q axes machine model with voltage/frequency dependent loads. To guarantee that design requirements and necessary trade studies are done, a functional analysis tool CORE is used for the study. This provides us with different control options for stability assessment. Initial studies and recommendations using advanced simulation tools are also presented. The benefits of the stability/control scheme for evaluating future designs and power management are discussed.

  7. Stabilizing Planar Inverted Pendulum System Based on Fuzzy Nine-point Controller

    OpenAIRE

    Qi Qian; Liu Feng; Tang Yong-chuan; Yang Yang

    2013-01-01

    In order to stabilize planar inverted pendulum, after analyzing the physical characteristics of the planar inverted pendulum system, a pendulum nine-point controller and a car nine-point controller for X-axis and Y-axis were designed respectively. Then a fuzzy coordinator was designed using the fuzzy control theory based on the priority of those two controllers, and the priority level of the pendulum is higher than the car. Thus, the control tasks of each controller in each axis were harmoniz...

  8. Robust Stability Analysis of the Space Launch System Control Design: A Singular Value Approach

    Science.gov (United States)

    Pei, Jing; Newsome, Jerry R.

    2015-01-01

    Classical stability analysis consists of breaking the feedback loops one at a time and determining separately how much gain or phase variations would destabilize the stable nominal feedback system. For typical launch vehicle control design, classical control techniques are generally employed. In addition to stability margins, frequency domain Monte Carlo methods are used to evaluate the robustness of the design. However, such techniques were developed for Single-Input-Single-Output (SISO) systems and do not take into consideration the off-diagonal terms in the transfer function matrix of Multi-Input-Multi-Output (MIMO) systems. Robust stability analysis techniques such as H(sub infinity) and mu are applicable to MIMO systems but have not been adopted as standard practices within the launch vehicle controls community. This paper took advantage of a simple singular-value-based MIMO stability margin evaluation method based on work done by Mukhopadhyay and Newsom and applied it to the SLS high-fidelity dynamics model. The method computes a simultaneous multi-loop gain and phase margin that could be related back to classical margins. The results presented in this paper suggest that for the SLS system, traditional SISO stability margins are similar to the MIMO margins. This additional level of verification provides confidence in the robustness of the control design.

  9. Assessment of effect of SSSC stabilizer in different control channels on damping inter-area oscillations

    Energy Technology Data Exchange (ETDEWEB)

    Shakarami, M.R., E-mail: shakarami@iust.ac.i [Centre of Excellence for Power System Automation and Operation, Department of Electrical Engineering, Iran University of Science and Technology, Narmak, Tehran (Iran, Islamic Republic of); Kazemi, A. [Centre of Excellence for Power System Automation and Operation, Department of Electrical Engineering, Iran University of Science and Technology, Narmak, Tehran (Iran, Islamic Republic of)

    2011-03-15

    A static synchronous series compensator (SSSC) is one of the series flexible ac transmission system (FACTS) devices that injects a balanced three-phase voltage in quadrature with the transmission line current. There are two channels for controlling of phase and magnitude of the voltage. When the SSSC is used for damping of inter-area oscillations, a SSSC-based stabilizer can be included in both channels. In this paper, the best location and suitable input control signal for SSSC in order to enhance the damping of inter-area oscillations are selected by residue analysis. A method by quadratic mathematic programming has been presented to the design of the stabilizer. By this method, the effect of the stabilizer in both control channels of the SSSC on damping of inter-area oscillations has been assessed. By considering the gain of stabilizer as a criterion, obtained results from studying on a small and a large multi-machine power system show that the stabilizer in the phase control channel is more effective for damping inter-are oscillations.

  10. Stability of the spreading in small-world network with predictive controller

    International Nuclear Information System (INIS)

    Bao, Z.J.; Jiang, Q.Y.; Yan, W.J.; Cao, Y.J.

    2010-01-01

    In this Letter, we apply the predictive control strategy to suppress the propagation of diseases or viruses in small-world network. The stability of small-world spreading model with predictive controller is investigated. The sufficient and necessary stability condition is given, which is closely related to the controller parameters and small-world rewiring probability p. Our simulations discover a phenomenon that, with the fixed predictive controller parameters, the spreading dynamics become more and more stable when p decreases from a larger value to a smaller one, and the suitable controller parameters can effectively suppress the spreading behaviors even when p varies within the whole spectrum, and the unsuitable controller parameters can lead to oscillation when p lies within a certain range.

  11. Nonlinear Feedback Control and Stability Analysis of a Proof-of-Work Blockchain

    Directory of Open Access Journals (Sweden)

    Geir Hovland

    2017-10-01

    Full Text Available In this paper a novel feedback controller and stability analysis of a blockchain implementation is developed by using a control engineering perspective. The controller output equals the difficulty adjustment in the mining process while the feedback variable is the average block time over a certain time period. The computational power (hash rate of the miners is considered a disturbance in the model. The developed controller is tested against a simulation model with constant disturbance, step and ramp responses as well as with a high-frequency sinusoidal disturbance. Stability and a fast response is demonstrated in all these cases with a controller which adjusts it's output at every new block. Finally the performance of the controller is implemented and demonstrated on a testnet with a constant hash rate as well as on the mainnet of a public open source blockchain project.

  12. A novel vehicle dynamics stability control algorithm based on the hierarchical strategy with constrain of nonlinear tyre forces

    Science.gov (United States)

    Li, Liang; Jia, Gang; Chen, Jie; Zhu, Hongjun; Cao, Dongpu; Song, Jian

    2015-08-01

    Direct yaw moment control (DYC), which differentially brakes the wheels to produce a yaw moment for the vehicle stability in a steering process, is an important part of electric stability control system. In this field, most control methods utilise the active brake pressure with a feedback controller to adjust the braked wheel. However, the method might lead to a control delay or overshoot because of the lack of a quantitative project relationship between target values from the upper stability controller to the lower pressure controller. Meanwhile, the stability controller usually ignores the implementing ability of the tyre forces, which might be restrained by the combined-slip dynamics of the tyre. Therefore, a novel control algorithm of DYC based on the hierarchical control strategy is brought forward in this paper. As for the upper controller, a correctional linear quadratic regulator, which not only contains feedback control but also contains feed forward control, is introduced to deduce the object of the stability yaw moment in order to guarantee the yaw rate and side-slip angle stability. As for the medium and lower controller, the quantitative relationship between the vehicle stability object and the target tyre forces of controlled wheels is proposed to achieve smooth control performance based on a combined-slip tyre model. The simulations with the hardware-in-the-loop platform validate that the proposed algorithm can improve the stability of the vehicle effectively.

  13. Controls on hillslope stability in a mountain river catchment

    Science.gov (United States)

    Golly, Antonius; Turowski, Jens; Hovius, Niels; Badoux, Alexandre

    2015-04-01

    Sediment transport in fluvial systems accounts for a large fraction of natural hazard damage costs in mountainous regions and is an important factor for risk mitigation, engineering and ecology. Although sediment transport in high-gradient channels gathered research interest over the last decades, sediment dynamics in steep streams are generally not well understood. For instance, the sourcing of the sediment and when and how it is actually mobilized is largely undescribed. In the Erlenbach, a mountain torrent in the Swiss Prealps, we study the mechanistic relations between in-channel hydrology, channel morphology, external climatic controls and the surrounding sediment sources to identify relevant process domains for sediment input and their characteristic scales. Here, we analyze the motion of a slow-moving landslide complex that was permanently monitored by time-lapse cameras over a period of 70 days at a 30 minutes interval. In addition, data sets for stream discharge, air temperature and precipitation rates are available. Apparent changes in the channel morphology, e.g. the destruction of channel-spanning bed forms, were manually determined from the time-lapse images and were treated as event marks in the time series. We identify five relevant types of sediment displacement processes emerging during the hillslope motion: concentrated mud flows, deep seated hillslope failure, catastrophic cavity failure, hillslope bank erosion and individual grain loss. Generally, sediment displacement occurs on a large range of temporal and spatial scales and sediment dynamics in steep streams not only depend on large floods with long recurrence intervals. We find that each type of displacement acts in a specific temporal and spatial domain with their characteristic scales. Different external climatic forcing (e.g. high-intensity vs. long-lasting precipitation events) promote different displacement processes. Stream morphology and the presence of boulders have a large effect on

  14. On the internal stability of non-linear dynamic inversion: application to flight control

    Czech Academy of Sciences Publication Activity Database

    Alam, M.; Čelikovský, Sergej

    2017-01-01

    Roč. 11, č. 12 (2017), s. 1849-1861 ISSN 1751-8644 R&D Projects: GA ČR(CZ) GA17-04682S Institutional support: RVO:67985556 Keywords : flight control * non-linear dynamic inversion * stability Subject RIV: BC - Control Systems Theory OBOR OECD: Automation and control systems Impact factor: 2.536, year: 2016 http://library.utia.cas.cz/separaty/2017/TR/celikovsky-0476150.pdf

  15. Global stabilization of linear continuous time-varying systems with bounded controls

    International Nuclear Information System (INIS)

    Phat, V.N.

    2004-08-01

    This paper deals with the problem of global stabilization of a class of linear continuous time-varying systems with bounded controls. Based on the controllability of the nominal system, a sufficient condition for the global stabilizability is proposed without solving any Riccati differential equation. Moreover, we give sufficient conditions for the robust stabilizability of perturbation/uncertain linear time-varying systems with bounded controls. (author)

  16. Dynamic stability control in forward falls: postural corrections after muscle fatigue in young and older adults.

    Science.gov (United States)

    Mademli, Lida; Arampatzis, Adamantios; Karamanidis, Kiros

    2008-06-01

    Many studies report that muscle strength loss may alter the human system's capacity to generate rapid force for balance corrections after perturbations, leading to deficient recovery behaviours. Yet little is known regarding the effect of modifications in the neuromuscular system induced by fatigue on dynamic stability control during postural perturbations. This study investigates the effect of muscle strength decline induced by fatiguing contractions on the dynamic stability control of young and older adults during forward falls. Eleven young and eleven older male adults had to regain balance after sudden falls before and after submaximal fatiguing knee extension-flexion contractions. Young subjects had a higher margin of stability than older ones before and after the fatiguing task. This reflects their enhanced ability in using mechanisms for maintaining dynamic stability (i.e. a greater base of support). The margin of stability, the boundary of the base of support and the position of the extrapolated centre of mass, remained unaffected by the reduction in muscle strength induced by the fatiguing contractions, indicating an appropriate adjustment of the motor commands to compensate the deficit in muscle strength. Both young and older adults were able to counteract the decreased horizontal ground reaction forces after the fatiguing task by flexing their knee to a greater extent, leading to similar decreases in the horizontal velocity of centre of mass as in the pre fatigue condition. The results demonstrate the ability of the central nervous system to rapidly modify the execution of postural corrections including mechanisms for maintaining dynamic stability.

  17. Optimization of a pressure control valve for high power automatic transmission considering stability

    Science.gov (United States)

    Jian, Hongchao; Wei, Wei; Li, Hongcai; Yan, Qingdong

    2018-02-01

    The pilot-operated electrohydraulic clutch-actuator system is widely utilized by high power automatic transmission because of the demand of large flowrate and the excellent pressure regulating capability. However, a self-excited vibration induced by the inherent non-linear characteristics of valve spool motion coupled with the fluid dynamics can be generated during the working state of hydraulic systems due to inappropriate system parameters, which causes sustaining instability in the system and leads to unexpected performance deterioration and hardware damage. To ensure a stable and fast response performance of the clutch actuator system, an optimal design method for the pressure control valve considering stability is proposed in this paper. A non-linear dynamic model of the clutch actuator system is established based on the motion of the valve spool and coupling fluid dynamics in the system. The stability boundary in the parameter space is obtained by numerical stability analysis. Sensitivity of the stability boundary and output pressure response time corresponding to the valve parameters are identified using design of experiment (DOE) approach. The pressure control valve is optimized using particle swarm optimization (PSO) algorithm with the stability boundary as constraint. The simulation and experimental results reveal that the optimization method proposed in this paper helps in improving the response characteristics while ensuring the stability of the clutch actuator system during the entire gear shift process.

  18. Carrier-envelope phase stabilization and control using a transmission grating compressor and an AOPDF.

    Science.gov (United States)

    Canova, Lorenzo; Chen, Xiaowei; Trisorio, Alexandre; Jullien, Aurélie; Assion, Andreas; Tempea, Gabriel; Forget, Nicolas; Oksenhendler, Thomas; Lopez-Martens, Rodrigo

    2009-05-01

    Carrier-envelope phase (CEP) stabilization of a femtosecond chirped-pulse amplification system featuring a compact transmission grating compressor is demonstrated. The system includes two amplification stages and routinely generates phase-stable (approximately 250 mrad rms) 2 mJ, 25 fs pulses at 1 kHz. Minimizing the optical pathway in the compressor enables phase stabilization without feedback control of the grating separation or beam pointing. We also demonstrate for the first time to the best of our knowledge, out-of-loop control of the CEP using an acousto-optic programmable dispersive filter inside the laser chain.

  19. A new strategy for transient stability using augmented generator control and local dynamic braking

    Energy Technology Data Exchange (ETDEWEB)

    Dorsey, J; Jiang, H; Habetler, T [Georgia Inst. of Tech., Atlanta, GA (United States); Qu, Z [University of Central Florida, Orlando, FL (United States)

    1994-12-31

    A decentralized automatic control strategy for significantly improving the transient stability of a large power system is introduced. The strategy combines local dynamic braking and a straightforward augmentation of the existing turbine / governor control system that uses only local feedback. The brake resistor, which employs thick film, metal oxide technology, has no inductance and is of very low resistance, allowing its use during fault to show a generator`s acceleration. Simulation results using the 39 Bus New England system show that the strategy dramatically increases the global stability of a power system. (author) 15 refs., 7 figs., 1 tab.

  20. Automatic approach to stabilization and control for multi robot teams by multilayer network operator

    Directory of Open Access Journals (Sweden)

    Diveev Askhat

    2016-01-01

    Full Text Available The paper describes a novel methodology for synthesis a high-level control of autonomous multi robot teams. The approach is based on multilayer network operator method that belongs to a symbolic regression class. Synthesis is accomplished in three steps: stabilizing robots about some given position in a state space, finding optimal trajectories of robots’ motion as sets of stabilizing points and then approximating all the points of optimal trajectories by some multi-dimensional function of state variables. The feasibility and effectiveness of the proposed approach is verified on simulations of the task of control synthesis for three mobile robots parking in the constrained space.

  1. Sliding Mode Controller and Lyapunov Redesign Controller to Improve Microgrid Stability: A Comparative Analysis with CPL Power Variation

    Directory of Open Access Journals (Sweden)

    Eklas Hossain

    2017-11-01

    Full Text Available To mitigate the microgrid instability despite the presence of dense Constant Power Load (CPL loads in the system, a number of compensation techniques have already been gone through extensive research, proposed, and implemented around the world. In this paper, a storage based load side compensation technique is used to enhance stability of microgrids. Besides adopting this technique here, Sliding Mode Controller (SMC and Lyapunov Redesign Controller (LRC, two of the most prominent nonlinear control techniques, are individually implemented to control microgrid system stability with desired robustness. CPL power is then varied to compare robustness of these two control techniques. This investigation revealed the better performance of the LRC system compared to SMC to retain stability in microgrid with dense CPL load. All the necessary results are simulated in Matlab/Simulink platform for authentic verification. Reasons behind inferior SMC performance and ways to mitigate that are also discussed. Finally, the effectiveness of SMC and LRC systems to attain stability in real microgrids is verified by numerical analysis.

  2. Proportional Load Sharing and Stability of DC Microgrid with Distributed Architecture Using SM Controller

    Directory of Open Access Journals (Sweden)

    Muhammad Rashad

    2018-01-01

    Full Text Available DC microgrids look attractive in distribution systems due to their high reliability, high efficiency, and easy integration with renewable energy sources. The key objectives of the DC microgrid include proportional load sharing and precise voltage regulation. Droop controllers are based on decentralized control architectures which are not effective in achieving these objectives simultaneously due to the voltage error and load power variation. A centralized controller can achieve these objectives using a high speed communication link. However, it loses reliability due to the single point failure. Additionally, these controllers are realized through proportional integral (PI controllers which cannot ensure load sharing and stability in all operating conditions. To address limitations, a distributed architecture using sliding mode (SM controller utilizing low bandwidth communication is proposed for DC microgrids in this paper. The main advantages are high reliability, load power sharing, and precise voltage regulation. Further, the SM controller shows high robustness, fast dynamic response, and good stability for large load variations. To analyze the stability and dynamic performance, a system model is developed and its transversality, reachability, and equivalent control conditions are verified. Furthermore, the dynamic behavior of the modeled system is investigated for underdamped and critically damped responses. Detailed simulations are carried out to show the effectiveness of the proposed controller.

  3. Fuzzy-Based Hybrid Control Algorithm for the Stabilization of a Tri-Rotor UAV.

    Science.gov (United States)

    Ali, Zain Anwar; Wang, Daobo; Aamir, Muhammad

    2016-05-09

    In this paper, a new and novel mathematical fuzzy hybrid scheme is proposed for the stabilization of a tri-rotor unmanned aerial vehicle (UAV). The fuzzy hybrid scheme consists of a fuzzy logic controller, regulation pole-placement tracking (RST) controller with model reference adaptive control (MRAC), in which adaptive gains of the RST controller are being fine-tuned by a fuzzy logic controller. Brushless direct current (BLDC) motors are installed in the triangular frame of the tri-rotor UAV, which helps maintain control on its motion and different altitude and attitude changes, similar to rotorcrafts. MRAC-based MIT rule is proposed for system stability. Moreover, the proposed hybrid controller with nonlinear flight dynamics is shown in the presence of translational and rotational velocity components. The performance of the proposed algorithm is demonstrated via MATLAB simulations, in which the proposed fuzzy hybrid controller is compared with the existing adaptive RST controller. It shows that our proposed algorithm has better transient performance with zero steady-state error, and fast convergence towards stability.

  4. Intelligent Control of UPFC for Enhancing Transient Stability on Multi-Machine Power Systems

    Directory of Open Access Journals (Sweden)

    Hassan Barati

    2010-01-01

    Full Text Available One of the benefit of FACTS devices is increase of stability in power systems with control active and reactive power at during the fault in power system. Although, the power system stabilizers (PSSs have been one of the most common controls used to damp out oscillations, this device may not produce enough damping especially to inter-area mode and therefore, there is an increasing interest in using FACTS devices to aid in damping of these oscillations. In This paper, UPFC is used for damping oscillations and to enhance the transient stability performance of power systems. The controller parameters are designed using an efficient version of the Takagi-Sugeno fuzzy control scheme. The function based Takagi-Sugeno-Kang (TSK fuzzy controller uses. For optimization parameters of fuzzy PI controller, the GA, PSO and HGAPSO algorithms are used. The computer simulation results, the effect of UPFC with conventional PI controller, fuzzy PI controller and intelligent controllers (GA, PSO and HGAPSO for damping the local-mode and inter-area mode of under large and small disturbances in the four-machine two-area power system evaluated and compared.

  5. Fuzzy Logic Based Control of the Lateral Stability of Tractor Semitrailer Vehicle

    Directory of Open Access Journals (Sweden)

    Xiujian Yang

    2015-01-01

    Full Text Available A novel control scheme is proposed to improve the yaw stability of a tractor semitrailer vehicle in critical situations. The control scheme is a two-layer structure consisting of an upper yaw moment controller and a lower brake force distributor. The tractor and the trailer are, respectively, stabilized by two independent fuzzy logic based yaw moment controllers. The controllers for the tractor and the trailer are, respectively, designed to track the reference yaw rate of the tractor and the hitch angle between the tractor and the trailer while considering the variation of the hitch angular rate at the same time. The corrective yaw moments determined by the corresponding upper fuzzy yaw moment controllers are realized by active wheel braking. The performance of the proposed control scheme is evaluated by simulations on a nonlinear vehicle model. The results demonstrate that the proposed control scheme is robust and effective in stabilizing the severe instabilities such as jackknife and trailer oscillation in the chosen simulation scenarios. It is believed that this control scheme is robust to the variation of road adhesion conditions.

  6. 55th Annual Canadian Society for Molecular Biosciences Conference on Epigenetics and Genomic Stability. Whistler, British Columbia, Canada, 14–18 March 2012.

    Science.gov (United States)

    Nelson, Christopher J; Ausió, Juan

    2012-06-01

    The 55th Annual Canadian Society for Molecular Biosciences Conference on Epigenetics and Genomic Stability in Whistler, Canada, 14-18 March 2012, brought together 31 speakers from different nationalities. The organizing committee, led by Jim Davie (Chair) at the University of Manitoba (Manitoba, Canada), consisted of several established researchers in the fields of chromatin and epigenetics from across Canada. The meeting was centered on the contribution of epigenetics to gene expression, DNA damage and repair, and the role of environmental factors. A few interesting talks on replication added some insightful information on the controversial issue of histone post-translational modifications as genuine epigenetic marks that are inherited through cell division.

  7. Long-Term Stability of Human Genomic and Human Papillomavirus DNA Stored in BD SurePath and Hologic PreservCyt Liquid-Based Cytology Media

    Science.gov (United States)

    Agreda, Patricia M.; Beitman, Gerard H.; Gutierrez, Erin C.; Harris, James M.; Koch, Kristopher R.; LaViers, William D.; Leitch, Sharon V.; Maus, Courtney E.; McMillian, Ray A.; Nussbaumer, William A.; Palmer, Marcus L. R.; Porter, Michael J.; Richart, Gregory A.; Schwab, Ryan J.

    2013-01-01

    We evaluated the effect of storage at 2 to 8°C on the stability of human genomic and human papillomavirus (HPV) DNA stored in BD SurePath and Hologic PreservCyt liquid-based cytology media. DNA retained the ability to be extracted and PCR amplified for more than 2.5 years in both medium types. Prior inability to detect DNA in archived specimens may have been due to failure of the extraction method to isolate DNA from fixed cells. PMID:23678069

  8. Exponential Stability of Time-Switched Two-Subsystem Nonlinear Systems with Application to Intermittent Control

    Directory of Open Access Journals (Sweden)

    Huang Tingwen

    2009-01-01

    Full Text Available This paper studies the exponential stability of a class of periodically time-switched nonlinear systems. Three cases of such systems which are composed, respectively, of a pair of unstable subsystems, of both stable and unstable subsystems, and of a pair of stable systems, are considered. For the first case, the proposed result shows that there exists periodically switching rule guaranteeing the exponential stability of the whole system with (sufficient small switching period if there is a Hurwitz linear convex combination of two uncertain linear systems derived from two subsystems by certain linearization. For the second case, we present two general switching criteria by means of multiple and single Lyapunov function, respectively. We also investigate the stability issue of the third case, and the switching criteria of exponential stability are proposed. The present results for the second case are further applied to the periodically intermittent control. Several numerical examples are also given to show the effectiveness of theoretical results.

  9. Stability and dynamics of a controlled van der Pol-Duffing oscillator

    International Nuclear Information System (INIS)

    Ji, J.C.; Hansen, C.H.

    2006-01-01

    The trivial equilibrium of a van der Pol-Duffing oscillator under a linear-plus-nonlinear feedback control may change its stability either via a single or via a double Hopf bifurcation if the time delay involved in the feedback reaches certain values. It is found that the trivial equilibrium may lose its stability via a subcritical or supercritical Hopf bifurcation and regain its stability via a reverse subcritical or supercritical Hopf bifurcation as the time delay increases. A stable limit cycle appears after a supercritical Hopf bifurcation occurs and disappears through a reverse supercritical Hopf bifurcation. The interaction of the weakly periodic excitation and the stable bifurcating solution is investigated for the forced system under primary resonance conditions. It is shown that the forced periodic response may lose its stability via a Neimark-Sacker bifurcation. Analytical results are validated by a comparison with those of direct numerical integration

  10. Postural stability and ankle sprain history in athletes compared to uninjured controls.

    Science.gov (United States)

    Huurnink, Arnold; Fransz, Duncan P; Kingma, Idsart; Verhagen, Evert A L M; van Dieën, Jaap H

    2014-02-01

    Diminished postural stability is a risk factor for ankle sprain occurrence and ankle sprains result in impaired postural stability. To date, ankle sprain history has not been taken into account as a determinant of postural stability, while it could possibly specify subgroups of interest. Postural stability was compared between 18 field hockey athletes who had recovered from an ankle sprain (mean (SD); 3.6 (1.5) months post-injury), and 16 uninjured controls. Force plate and kinematics parameters were calculated during single-leg standing: mean center of pressure speed, mean absolute horizontal ground reaction force, mean absolute ankle angular velocity, and mean absolute hip angular velocity. Additionally, cluster analysis was applied to the 'injured' participants, and the cluster with diminished postural stability was compared to the other participants with respect to ankle sprain history. MANCOVA showed no significant difference between groups in postural stability (P = 0.68). A self-reported history of an (partial) ankle ligament rupture was typically present in the cluster with diminished postural stability. Subsequently, a 'preceding rupture' was added as a factor in the MANCOVA, which showed a significant association between diminished postural stability and a 'preceding rupture' (P = 0.01), for all four individual parameters (P: 0.001-0.029; Cohen's d: 0.96-2.23). Diminished postural stability is not apparent in all previously injured athletes. However, our analysis suggests that an (mild) ankle sprain with a preceding severe ankle sprain is associated with impaired balance ability. Therefore, sensorimotor training may be emphasized in this particular group and caution is warranted in return to play decisions. Copyright © 2013 Elsevier Ltd. All rights reserved.

  11. Enhancing the stabilization of aircraft pitch motion control via intelligent and classical method

    Science.gov (United States)

    Lukman, H.; Munawwarah, S.; Azizan, A.; Yakub, F.; Zaki, S. A.; Rasid, Z. A.

    2017-12-01

    The pitching movement of an aircraft is very important to ensure passengers are intrinsically safe and the aircraft achieve its maximum stability. The equations governing the motion of an aircraft are a complex set of six nonlinear coupled differential equations. Under certain assumptions, it can be decoupled and linearized into longitudinal and lateral equations. Pitch control is a longitudinal problem and thus, only the longitudinal dynamics equations are involved in this system. It is a third order nonlinear system, which is linearized about the operating point. The system is also inherently unstable due to the presence of a free integrator. Because of this, a feedback controller is added in order to solve this problem and enhance the system performance. This study uses two approaches in designing controller: a conventional controller and an intelligent controller. The pitch control scheme consists of proportional, integral and derivatives (PID) for conventional controller and fuzzy logic control (FLC) for intelligent controller. Throughout the paper, the performance of the presented controllers are investigated and compared based on the common criteria of step response. Simulation results have been obtained and analysed by using Matlab and Simulink software. The study shows that FLC controller has higher ability to control and stabilize the aircraft's pitch angle as compared to PID controller.

  12. Tracking performance and global stability guaranteed neural control of uncertain hypersonic flight vehicle

    Directory of Open Access Journals (Sweden)

    Tao Teng

    2016-02-01

    Full Text Available In this article, a global adaptive neural dynamic surface control with predefined tracking performance is developed for a class of hypersonic flight vehicles, whose accurate dynamics is hard to obtain. The control scheme developed in this paper overcomes the limitations of neural approximation region by employing a switching mechanism which incorporates an additional robust controller outside the neural approximation region to pull the transient state variables back when they overstep the neural approximation region, such that globally uniformly ultimately bounded stability can be guaranteed. Especially, the developed global adaptive neural control also improves the tracking performance by introducing an error transformation mechanism, such that both transient and steady-state performance can be shaped according to the predefined bounds. Simulation studies on the hypersonic flight vehicle validate that the designed controller has good velocity modulation and velocity stability performance.

  13. Design of Launch Vehicle Flight Control Systems Using Ascent Vehicle Stability Analysis Tool

    Science.gov (United States)

    Jang, Jiann-Woei; Alaniz, Abran; Hall, Robert; Bedossian, Nazareth; Hall, Charles; Jackson, Mark

    2011-01-01

    A launch vehicle represents a complicated flex-body structural environment for flight control system design. The Ascent-vehicle Stability Analysis Tool (ASAT) is developed to address the complicity in design and analysis of a launch vehicle. The design objective for the flight control system of a launch vehicle is to best follow guidance commands while robustly maintaining system stability. A constrained optimization approach takes the advantage of modern computational control techniques to simultaneously design multiple control systems in compliance with required design specs. "Tower Clearance" and "Load Relief" designs have been achieved for liftoff and max dynamic pressure flight regions, respectively, in the presence of large wind disturbances. The robustness of the flight control system designs has been verified in the frequency domain Monte Carlo analysis using ASAT.

  14. Offshore Wind Farms and HVDC Grids Modeling as a Feedback Control System for Stability Analysis

    DEFF Research Database (Denmark)

    Bidadfar, Ali; Saborío-Romano, Oscar; Altin, Müfit

    The low impedance characteristics of DC transmission lines cause the voltage source converter (VSC) in HVDC networks to become electrically closer together and increase the risk of severe interactions between the converters. Such interactions, in turn, intensify the implementation of the grid...... control schemes and may lead the entire system to instability. Assessing the stability and adopting complex coordinated control schemes in an HVDC grid and wind farm turbines are challenging and require a precise model of the HVDC grid, wind farm, and the controllers. In this paper, a linear multivariable...... feedback control system (FCS) model is proposed to represent the dynamic characteristics of HVDC grids and their controllers. The FCS model can be used for different dynamic analyses in time and frequency domains. Moreover, using the FCS model the system stability is analyzed in both open- and closed...

  15. Robust Stabilization of Fractional-Order Systems with Interval Uncertainties via Fractional-Order Controllers

    Directory of Open Access Journals (Sweden)

    Sayyad Delshad Saleh

    2010-01-01

    Full Text Available Abstract We propose a fractional-order controller to stabilize unstable fractional-order open-loop systems with interval uncertainty whereas one does not need to change the poles of the closed-loop system in the proposed method. For this, we will use the robust stability theory of Fractional-Order Linear Time Invariant (FO-LTI systems. To determine the control parameters, one needs only a little knowledge about the plant and therefore, the proposed controller is a suitable choice in the control of interval nonlinear systems and especially in fractional-order chaotic systems. Finally numerical simulations are presented to show the effectiveness of the proposed controller.

  16. Behavior of QQ-plots and genomic control in studies of gene-environment interaction.

    Directory of Open Access Journals (Sweden)

    Arend Voorman

    2011-05-01

    Full Text Available Genome-wide association studies of gene-environment interaction (GxE GWAS are becoming popular. As with main effects GWAS, quantile-quantile plots (QQ-plots and Genomic Control are being used to assess and correct for population substructure. However, in G x E work these approaches can be seriously misleading, as we illustrate; QQ-plots may give strong indications of substructure when absolutely none is present. Using simulation and theory, we show how and why spurious QQ-plot inflation occurs in G x E GWAS, and how this differs from main-effects analyses. We also explain how simple adjustments to standard regression-based methods used in G x E GWAS can alleviate this problem.

  17. Stabilization of business cycles of finance agents using nonlinear optimal control

    Science.gov (United States)

    Rigatos, G.; Siano, P.; Ghosh, T.; Sarno, D.

    2017-11-01

    Stabilization of the business cycles of interconnected finance agents is performed with the use of a new nonlinear optimal control method. First, the dynamics of the interacting finance agents and of the associated business cycles is described by a modeled of coupled nonlinear oscillators. Next, this dynamic model undergoes approximate linearization round a temporary operating point which is defined by the present value of the system's state vector and the last value of the control inputs vector that was exerted on it. The linearization procedure is based on Taylor series expansion of the dynamic model and on the computation of Jacobian matrices. The modelling error, which is due to the truncation of higher-order terms in the Taylor series expansion is considered as a disturbance which is compensated by the robustness of the control loop. Next, for the linearized model of the interacting finance agents, an H-infinity feedback controller is designed. The computation of the feedback control gain requires the solution of an algebraic Riccati equation at each iteration of the control algorithm. Through Lyapunov stability analysis it is proven that the control scheme satisfies an H-infinity tracking performance criterion, which signifies elevated robustness against modelling uncertainty and external perturbations. Moreover, under moderate conditions the global asymptotic stability features of the control loop are proven.

  18. Observer enhanced control for spin-stabilized tethered formation in earth orbit

    Science.gov (United States)

    Guang, Zhai; Yuyang, Li; Liang, Bin

    2018-04-01

    This paper addresses the issues relevant to control of spin-stabilized tethered formation in circular orbit. Due to the dynamic complexities and nonlinear perturbations, it is challenging to promote the control precision for the formation deployment and maintenance. In this work, the formation dynamics are derived with considering the spinning rate of the central body, then major attention is dedicated to develop the nonlinear disturbance observer. To achieve better control performance, the observer-enhanced controller is designed by incorporating the disturbance observer into the control loop, benefits from the disturbance compensation are demonstrated, and also, the dependences of the disturbance observer performance on some important parameters are theoretically and numerically analyzed.

  19. Stabilization Using a Discrete Fuzzy PDC Control with PID Controllers and Pole Placement: Application to an Experimental Greenhouse

    Directory of Open Access Journals (Sweden)

    Amine Chouchaine

    2011-01-01

    Full Text Available This paper proposes a control strategy for complex and nonlinear systems, based on a parallel distributed compensation (PDC controller. A solution is presented to solve a stability problem that arises when dealing with a Takagi-Sugeno discrete system with great numbers of rules. The PDC controller will use a classical controller like a PI, PID, or RST in each rule with a pole placement strategy to avoid causing instability. The fuzzy controller presented combines the multicontrol approach and the performance of the classical controllers to obtain a robust nonlinear control action that can also deal with time-variant systems. The presented method was applied to a small greenhouse to control its inside temperature by variation in ventilation rate inside the process. The results obtained will show the efficiency of the adopted method to control the nonlinear and complex systems.

  20. Natural selection among Eurasians at genomic regions associated with HIV-1 control

    Directory of Open Access Journals (Sweden)

    Allison David B

    2011-06-01

    Full Text Available Abstract Background HIV susceptibility and pathogenicity exhibit both interindividual and intergroup variability. The etiology of intergroup variability is still poorly understood, and could be partly linked to genetic differences among racial/ethnic groups. These genetic differences may be traceable to different regimes of natural selection in the 60,000 years since the human radiation out of Africa. Here, we examine population differentiation and haplotype patterns at several loci identified through genome-wide association studies on HIV-1 control, as determined by viral-load setpoint, in European and African-American populations. We use genome-wide data from the Human Genome Diversity Project, consisting of 53 world-wide populations, to compare measures of FST and relative extended haplotype homozygosity (REHH at these candidate loci to the rest of the respective chromosome. Results We find that the Europe-Middle East and Europe-South Asia pairwise FST in the most strongly associated region are elevated compared to most pairwise comparisons with the sub-Saharan African group, which exhibit very low FST. We also find genetic signatures of recent positive selection (higher REHH at these associated regions among all groups except for sub-Saharan Africans and Native Americans. This pattern is consistent with one in which genetic differentiation, possibly due to diversifying/positive selection, occurred at these loci among Eurasians. Conclusions These findings are concordant with those from earlier studies suggesting recent evolutionary change at immunity-related genomic regions among Europeans, and shed light on the potential genetic and evolutionary origin of population differences in HIV-1 control.

  1. Robust Stability and H∞ Control of Uncertain Piecewise Linear Switched Systems with Filippov Solutions

    DEFF Research Database (Denmark)

    Ahmadi, Mohamadreza; Mojallali, Hamed; Wisniewski, Rafal

    2012-01-01

    This paper addresses the robust stability and control problem of uncertain piecewise linear switched systems where, instead of the conventional Carathe ́odory solutions, we allow for Filippov solutions. In other words, in contrast to the previous studies, solutions with infinite switching in fini...... algorithm is proposed to surmount the aforementioned matrix inequality conditions....... time along the facets and on faces of arbitrary dimensions are also taken into account. Firstly, based on earlier results, the stability problem of piecewise linear systems with Filippov solutions is translated into a number of linear matrix inequality feasibility tests. Subsequently, a set of matrix...... inequalities are brought forward, which determines the asymptotic stability of the Filippov solutions of a given uncertain piecewise linear system. Afterwards, bilinear matrix inequality conditions for synthesizing a robust controller with a guaranteed H∞ per- formance are formulated. Finally, a V-K iteration...

  2. Asymptotic stability and disturbance attenuation properties for a class of networked control systems

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    In this paper, stability and disturbance attenuation issues for a class of Networked Control Systems (NCSs)under uncertain access delay and packet dropout effects are considered. Our aim is to find conditions on the delay and packet dropout rate, under which the system stability and H∞ disturbance attenuation properties are preserved to a desired level. The basic idea in this paper is to formulate such Networked Control System as a discrete-time switched system. Then the NCSs' stability and performance problems can be reduced to the corresponding problems for switched systems, which have been studied for decades and for which a number of results are available in the literature. The techniques in this paper are based on recent progress in the discrete-time switched systems and piecewise Lyapunov functions.

  3. Stability Control of Force-Reflected Nonlinear Multilateral Teleoperation System under Time-Varying Delays

    Directory of Open Access Journals (Sweden)

    Da Sun

    2016-01-01

    Full Text Available A novel control algorithm based on the modified wave-variable controllers is proposed to achieve accurate position synchronization and reasonable force tracking of the nonlinear single-master-multiple-slave teleoperation system and simultaneously guarantee overall system’s stability in the presence of large time-varying delays. The system stability in different scenarios of human and environment situations has been analyzed. The proposed method is validated through experimental work based on the 3-DOF trilateral teleoperation system consisting of three different manipulators. The experimental results clearly demonstrate the feasibility of the proposed algorithm to achieve high transparency and robust stability in nonlinear single-master-multiple-slave teleoperation system in the presence of time-varying delays.

  4. Polynomial fuzzy model-based control systems stability analysis and control synthesis using membership function dependent techniques

    CERN Document Server

    Lam, Hak-Keung

    2016-01-01

    This book presents recent research on the stability analysis of polynomial-fuzzy-model-based control systems where the concept of partially/imperfectly matched premises and membership-function dependent analysis are considered. The membership-function-dependent analysis offers a new research direction for fuzzy-model-based control systems by taking into account the characteristic and information of the membership functions in the stability analysis. The book presents on a research level the most recent and advanced research results, promotes the research of polynomial-fuzzy-model-based control systems, and provides theoretical support and point a research direction to postgraduate students and fellow researchers. Each chapter provides numerical examples to verify the analysis results, demonstrate the effectiveness of the proposed polynomial fuzzy control schemes, and explain the design procedure. The book is comprehensively written enclosing detailed derivation steps and mathematical derivations also for read...

  5. Sliding Mode Controller and Lyapunov Redesign Controller to Improve Microgrid Stability:A Comparative Analysis with CPL Power Variation

    OpenAIRE

    Hossain, Eklas; Perez, Ron; Padmanaban, Sanjeevikumar; Mihet-Popa, Lucian; Blaabjerg, Frede; Ramachandaramurthy, Vigna K.

    2017-01-01

    To mitigate the microgrid instability despite the presence of dense Constant Power Load (CPL) loads in the system, a number of compensation techniques have already been gone through extensive research, proposed, and implemented around the world. In this paper, a storage based load side compensation technique is used to enhance stability of microgrids. Besides adopting this technique here, Sliding Mode Controller (SMC) and Lyapunov Redesign Controller (LRC), two of the most prominent nonlinear...

  6. Postural stability in patients with knee osteoarthritis: comparison with controls and evaluation of relationships between postural stability scores and International Classification of Functioning, Disability and Health components.

    Science.gov (United States)

    Hsieh, Ru-Lan; Lee, Wen-Chung; Lo, Min-Tzu; Liao, Wei-Cheng

    2013-02-01

    To assess the differences in postural stability between patients with knee osteoarthritis and controls without knee osteoarthritis, and to evaluate possible relations between postural stability scores and International Classification of Functioning, Disability and Health (ICF) components. An age-matched, case-controlled trial with a cross-sectional design. A teaching hospital. Patients with knee osteoarthritis (n=73) and age-matched controls (n=60). Data on patients' postural stability and additional health-related variables were collected using various instruments. These included the Hospital Anxiety and Depression Scale, the Multidimensional Fatigue Inventory, the World Health Organization Quality of Life Brief Version, the physical function test (chair-rising time), the Chinese version of the Western Ontario and McMaster Universities Osteoarthritis Index, the Chinese version of the Knee Injury and Osteoarthritis Outcome Score, and the Biodex Stability System. A comparison of postural stability in patients with knee osteoarthritis versus that of controls was performed. The relation between postural stability scores for patients with knee osteoarthritis and ICF components was evaluated. Pearson correlation tests were used to determine the variables that correlated with postural stability among these patients. Patients with knee osteoarthritis displayed lower overall postural stability than controls (scores of 0.7 vs. 0.5, P=.006) and scored lower on the environmental domain of the World Health Organization Quality of Life Brief Version (62.2 vs 66.8, P=.014). For patients with knee osteoarthritis, postural stability was weakly associated with the ICF components of body functions and structures, including pain (r=.33-.34, P=.004), physical fatigue (r=.28, P=.016), and reduced motivation (r=.30, P=.011). Weak to moderate associations between postural stability and the ICF components of activities and participation were found; the relevant ICF variables included

  7. Eight new genomes and synthetic controls increase the accessibility of rapid melt-MAMA SNP typing of Coxiella burnetii.

    Directory of Open Access Journals (Sweden)

    Edvin Karlsson

    Full Text Available The case rate of Q fever in Europe has increased dramatically in recent years, mainly because of an epidemic in the Netherlands in 2009. Consequently, there is a need for more extensive genetic characterization of the disease agent Coxiella burnetii in order to better understand the epidemiology and spread of this disease. Genome reference data are essential for this purpose, but only thirteen genome sequences are currently available. Current methods for typing C. burnetii are criticized for having problems in comparing results across laboratories, require the use of genomic control DNA, and/or rely on markers in highly variable regions. We developed in this work a method for single nucleotide polymorphism (SNP typing of C. burnetii isolates and tissue samples based on new assays targeting ten phylogenetically stable synonymous canonical SNPs (canSNPs. These canSNPs represent previously known phylogenetic branches and were here identified from sequence comparisons of twenty-one C. burnetii genomes, eight of which were sequenced in this work. Importantly, synthetic control templates were developed, to make the method useful to laboratories lacking genomic control DNA. An analysis of twenty-one C. burnetii genomes confirmed that the species exhibits high sequence identity. Most of its SNPs (7,493/7,559 shared by >1 genome follow a clonal inheritance pattern and are therefore stable phylogenetic typing markers. The assays were validated using twenty-six genetically diverse C. burnetii isolates and three tissue samples from small ruminants infected during the epidemic in the Netherlands. Each sample was assigned to a clade. Synthetic controls (vector and PCR amplified gave identical results compared to the corresponding genomic controls and are viable alternatives to genomic DNA. The results from the described method indicate that it could be useful for cheap and rapid disease source tracking at non-specialized laboratories, which requires accurate

  8. Stability Enhancement of Multi machine AC Systems by Synchronverter HVDC control

    Directory of Open Access Journals (Sweden)

    Raouia Aouini

    2016-06-01

    Full Text Available This paper investigates the impact of the Synchronverter based HVDC control on power system stability. The study considers multi machine power systems, with realistic parameters. A specific tuning method of the parameters of the regulators is used. The proposed control scheme is based on the sensitivity of the poles of the HVDC neighbor zone to the control parameters, and next, on their placement using residues. The transient stability of the HVDC neighbor zone is a priori taken into account at the design stage. The new tuning method is evaluated in comparison with the standard vector control via simulation tests. Extensive tests are performed using Matlab/Simulink implementation of the IEEE 9 bus/3 machines test system. The results prove the superiority of the proposed control to the classic vector control. The synchronverter control allows to improve not only the local performances of the HVDC link, but also the overall transient stability of the AC zone in which the HVDC is inserted. (where

  9. Temporal stability and transferability of models of willingness to pay for flood control and wetland conservation

    NARCIS (Netherlands)

    Brouwer, R.; Bateman, I.J.

    2005-01-01

    This study investigates the temporal stability and transferability of dichotomous choice willingness to pay responses and their determinants from two large-scale contingent valuation surveys in the area of flood control and wetland conservation. The study considers a time period between surveys

  10. Study on transient stability of wind turbine with induction generator based on variable pitch control strategy

    DEFF Research Database (Denmark)

    Zhao, B.; Li, H.; Han, L.

    2011-01-01

    In order to enhance and improve the transient stability of a grid-connected wind turbine generator system under the power grid fault, based on typical pitch control strategy of wind turbine, considering the wind turbine system oscillation caused by the drive-train shaft flexibility, Based on Matl...

  11. IQC-based robust stability analysis for LPV control of doubly-fed induction generators

    NARCIS (Netherlands)

    Tien, H. N.; Scherer, C. W.; Scherpen, J. M. A.

    2008-01-01

    Parameters of electrical machines are usually varying with time in a smooth way due to changing operating conditions, such as variations in the machine temperature and/or the magnetic saturation. This paper is concerned with robust stability analysis of controlled Doubly-Fed Induction Generators

  12. Asymptotic Stabilization of Non-holonomic Port-controlled Hamiltonian Systems

    DEFF Research Database (Denmark)

    Sørensen, Mathias Jesper; Bendtsen, Jan Dimon; Andersen, Palle

    2004-01-01

    A novel method for asymptotic stabilization of a class of non-holonomic systems is presented. The method is based on the port-controlled Hamiltonian description of electro-mechanical systems. The general system is augmented with so-called kinematic inputs, thus representing a special class of mob...

  13. Stability and control of Lur'e-type measure differential inclusions

    NARCIS (Netherlands)

    Wouw, van de N.; Leine, R.I.; Leonov, G.; Nijmeijer, H.; Pogromsky, A.; Fradkov, A.

    2010-01-01

    In this paper we present results on, firstly, the stability analysis for perturbed Lur'e-type measure differential inclusions and, secondly, the tracking control problem for this class of systems. The framework of measure differential inclusions allows us to describe systems with discontinuities in

  14. High stability vector-based direct power control for DFIG-based wind turbine

    DEFF Research Database (Denmark)

    Zhu, Rongwu; Chen, Zhe; Wu, Xiaojie

    2015-01-01

    This paper proposes an improved vector-based direct power control (DPC) strategy for the doubly-fed induction generator (DFIG)-based wind energy conversion system. Based on the small signal model, the proposed DPC improves the stability of the DFIG, and avoids the DFIG operating in the marginal...

  15. Recent Advances in Heliogyro Solar Sail Structural Dynamics, Stability, and Control Research

    Science.gov (United States)

    Wilkie, W. Keats; Warren, Jerry E.; Horta, Lucas G.; Lyle, Karen H.; Juang, Jer-Nan; Gibbs, S. Chad; Dowell, Earl H.; Guerrant, Daniel V.; Lawrence, Dale

    2015-01-01

    Results from recent NASA sponsored research on the structural dynamics, stability, and control characteristics of heliogyro solar sails are summarized. Specific areas under investigation include coupled nonlinear finite element analysis of heliogyro membrane blade with solar radiation pressure effects, system identification of spinning membrane structures, and solarelastic stability analysis of heliogyro solar sails, including stability during blade deployment. Recent results from terrestrial 1-g blade dynamics and control experiments on "rope ladder" membrane blade analogs, and small-scale in vacuo system identification experiments with hanging and spinning high-aspect ratio membranes will also be presented. A low-cost, rideshare payload heliogyro technology demonstration mission concept is used as a mission context for these heliogyro structural dynamics and solarelasticity investigations, and is also described. Blade torsional dynamic response and control are also shown to be significantly improved through the use of edge stiffening structural features or inclusion of modest tip masses to increase centrifugal stiffening of the blade structure. An output-only system identification procedure suitable for on-orbit blade dynamics investigations is also developed and validated using ground tests of spinning sub-scale heliogyro blade models. Overall, analytical and experimental investigations to date indicate no intractable stability or control issues for the heliogyro solar sail concept.

  16. Stability analysis of DC microgrids with constant power load under distributed control methods

    DEFF Research Database (Denmark)

    Liu, Zhangjie; Su, Mei; Sun, Yao

    2018-01-01

    of distributed controller is investigated. The small-signal model is established to predict the system qualitative behavior around equilibrium. The stability conditions of the system with time delay are derived based on the equivalent linearized model. Additionally, eigenvalue analysis based on inertia theorem...

  17. A Control Strategy for Flywheel Energy Storage System for Frequency Stability Improvement in Islanded Microgrid

    Directory of Open Access Journals (Sweden)

    A. A. Khodadoost Arani

    2017-03-01

    Full Text Available The Micro-Grid (MG stability is a significant issue that must be maintained in all operational modes. Usually, two control strategies can be applied to MG; V/f control and PQ control strategies. MGs with V/f control strategy should have some Distributed Generators (DGs which have fast responses versus load changes. The Flywheel Energy Storage System (FESS has this characteristic. The FESS, which converts the mechanical energy to electrical form, can generate electrical power or absorb the additional power in power systems or MGs. In this paper, the FESS structure modeled in detail and two control strategies (V/f and PQ control are applied for this application. In addition, in order to improve the MG frequency and voltage stability, two complementary controllers are proposed for the V/f control strategy; conventional PI and Fuzzy Controllers. A typical low voltage network, including FESS is simulated for four distinct scenarios in the MATLAB/ Simulink environment. It is shown that fuzzy controller has better performance than conventional PI controller in islanded microgrid.

  18. Multi-criteria decision making approaches for quality control of genome-wide association studies.

    Science.gov (United States)

    Malovini, Alberto; Rognoni, Carla; Puca, Annibale; Bellazzi, Riccardo

    2009-03-01

    Experimental errors in the genotyping phases of a Genome-Wide Association Study (GWAS) can lead to false positive findings and to spurious associations. An appropriate quality control phase could minimize the effects of this kind of errors. Several filtering criteria can be used to perform quality control. Currently, no formal methods have been proposed for taking into account at the same time these criteria and the experimenter's preferences. In this paper we propose two strategies for setting appropriate genotyping rate thresholds for GWAS quality control. These two approaches are based on the Multi-Criteria Decision Making theory. We have applied our method on a real dataset composed by 734 individuals affected by Arterial Hypertension (AH) and 486 nonagenarians without history of AH. The proposed strategies appear to deal with GWAS quality control in a sound way, as they lead to rationalize and make explicit the experimenter's choices thus providing more reproducible results.

  19. Thermal stability analysis and auxiliary power feedback control for the tokamak engineering test breeder (TETB-II)

    International Nuclear Information System (INIS)

    Sheng Guangzhao

    1993-01-01

    The thermal stability of TETB-II is analyzed using different methods, viz., POPCON, linear stability analysis and the time evolution calculation of plasma parameters. A thermal instability of the TETB-II is predicted. Auxiliary power feedback control for thermal stability appears feasible and efficient

  20. Controlled high-fidelity navigation in the charge stability diagram of a double quantum dot

    International Nuclear Information System (INIS)

    Coden, Diego S Acosta; Romero, Rodolfo H; Räsänen, Esa

    2015-01-01

    We propose an efficient control protocol for charge transfer in a double quantum dot. We consider numerically a two-dimensional model system, where the quantum dots are subjected to time-dependent electric fields corresponding to experimental gate voltages. Our protocol enables navigation in the charge stability diagram from a state to another through controllable variation of the fields. We show that the well-known adiabatic Landau–Zener transition—when supplemented with a time-dependent field tailored with optimal control theory—can remarkably improve the transition speed. The results also lead to a simple control scheme obtained from the experimental charge stability diagram that requires only a single parameter. Eventually, we can achieve the ultrafast performance of the composite pulse protocol that allows the system to be driven at the quantum speed limit. (paper)

  1. Axial stability of VVER-1000 reactor with control with minimum standard deviation

    International Nuclear Information System (INIS)

    Afanas'ev, A.M.; Torlin, B.Z.

    1980-01-01

    Results are given of investigations on the stability of a reactor which has, in addition to an automatic controller, a height distribution regulator (HDR) based on an auxiliary control rod (CR) or a special shortened absorption rod (SAR). The HDR was controlled by using either a special ionization chamber (IC), generating an imbalance signal which sets the CR in motion, or two ionization chambers whose difference signal causes a displacement of the SAR. Since data from numerous pickups can be used to control the height field of the VVER-1000, it is of interest to analyze how this would affect the stability of the reactor. The analysis was carried out with the improved IRINA programs. 11 refs

  2. Rapid feedback control and stabilization of an optical tweezers with a budget microcontroller

    International Nuclear Information System (INIS)

    Nino, Daniel; Wang, Haowei; N Milstein, Joshua

    2014-01-01

    Laboratories ranging the scientific disciplines employ feedback control to regulate variables within their experiments, from the flow of liquids within a microfluidic device to the temperature within a cell incubator. We have built an inexpensive, yet fast and rapidly deployed, feedback control system that is straightforward and flexible to implement from a commercially available Arduino Due microcontroller. This is in comparison with the complex, time-consuming and often expensive electronics that are commonly implemented. As an example of its utility, we apply our feedback controller to the task of stabilizing the main trapping laser of an optical tweezers. The feedback controller, which is inexpensive yet fast and rapidly deployed, was implemented from hacking an open source Arduino Due microcontroller. Our microcontroller based feedback system can stabilize the laser intensity to a few tenths of a per cent at 200 kHz, which is an order of magnitude better than the laser's base specifications, illustrating the utility of these devices. (paper)

  3. Improvement of the directional stability of passenger car trailer couplings with actively controlled steering

    Science.gov (United States)

    Desens, Jens

    The stabilization of pendulum oscillations of passenger car trailer couplings, using active steering, was examined. A linear model of the couplings was presented. Each axle was provided with a controller. The controllers were optimized, with regard to necessary sensors, in order to minimize costs. The rear and the front axles were provided with a control unit in order to compute the potential prevailing in the active steering of several axles. It was shown that the passenger car rear axle was the most suitable for coupling stabilization. The experiment was simulated, using a complex coupling model. The developed controller allowed the passenger car trailer to be driven at a speed higher than 150 km per hour.

  4. Rapid feedback control and stabilization of an optical tweezers with a budget microcontroller

    Energy Technology Data Exchange (ETDEWEB)

    Nino, Daniel; Wang, Haowei; N Milstein, Joshua, E-mail: josh.milstein@utoronto.ca [Department of Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, ON L5L 1C6 (Canada)

    2014-09-01

    Laboratories ranging the scientific disciplines employ feedback control to regulate variables within their experiments, from the flow of liquids within a microfluidic device to the temperature within a cell incubator. We have built an inexpensive, yet fast and rapidly deployed, feedback control system that is straightforward and flexible to implement from a commercially available Arduino Due microcontroller. This is in comparison with the complex, time-consuming and often expensive electronics that are commonly implemented. As an example of its utility, we apply our feedback controller to the task of stabilizing the main trapping laser of an optical tweezers. The feedback controller, which is inexpensive yet fast and rapidly deployed, was implemented from hacking an open source Arduino Due microcontroller. Our microcontroller based feedback system can stabilize the laser intensity to a few tenths of a per cent at 200 kHz, which is an order of magnitude better than the laser's base specifications, illustrating the utility of these devices. (paper)

  5. RecQL5 promotes genome stabilization through two parallel mechanisms--interacting with RNA polymerase II and acting as a helicase.

    Science.gov (United States)

    Islam, M Nurul; Fox, David; Guo, Rong; Enomoto, Takemi; Wang, Weidong

    2010-05-01

    The RecQL5 helicase is essential for maintaining genome stability and reducing cancer risk. To elucidate its mechanism of action, we purified a RecQL5-associated complex and identified its major component as RNA polymerase II (Pol II). Bioinformatics and structural modeling-guided mutagenesis revealed two conserved regions in RecQL5 as KIX and SRI domains, already known in transcriptional regulators for Pol II. The RecQL5-KIX domain binds both initiation (Pol IIa) and elongation (Pol IIo) forms of the polymerase, whereas the RecQL5-SRI domain interacts only with the elongation form. Fully functional RecQL5 requires both helicase activity and associations with the initiation polymerase, because mutants lacking either activity are partially defective in the suppression of sister chromatid exchange and resistance to camptothecin-induced DNA damage, and mutants lacking both activities are completely defective. We propose that RecQL5 promotes genome stabilization through two parallel mechanisms: by participation in homologous recombination-dependent DNA repair as a RecQ helicase and by regulating the initiation of Pol II to reduce transcription-associated replication impairment and recombination.

  6. Genetics and Molecular Biology of Epstein-Barr Virus-Encoded BART MicroRNA: A Paradigm for Viral Modulation of Host Immune Response Genes and Genome Stability

    Directory of Open Access Journals (Sweden)

    David H. Dreyfus

    2017-01-01

    Full Text Available Epstein-Barr virus, a ubiquitous human herpesvirus, is associated through epidemiologic evidence with common autoimmune syndromes and cancers. However, specific genetic mechanisms of pathogenesis have been difficult to identify. In this review, the author summarizes evidence that recently discovered noncoding RNAs termed microRNA encoded by Epstein-Barr virus BARF (BamHI A right frame termed BART (BamHI A right transcripts are modulators of human immune response genes and genome stability in infected and bystander cells. BART expression is apparently regulated by complex feedback loops with the host immune response regulatory NF-κB transcription factors. EBV-encoded BZLF-1 (ZEBRA protein could also regulate BART since ZEBRA contains a terminal region similar to ankyrin proteins such as IκBα that regulate host NF-κB. BALF-2 (BamHI A left frame transcript, a viral homologue of the immunoglobulin and T cell receptor gene recombinase RAG-1 (recombination-activating gene-1, may also be coregulated with BART since BALF-2 regulatory sequences are located near the BART locus. Viral-encoded microRNA and viral mRNA transferred to bystander cells through vesicles, defective viral particles, or other mechanisms suggest a new paradigm in which bystander or hit-and-run mechanisms enable the virus to transiently or chronically alter human immune response genes as well as the stability of the human genome.

  7. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability

    Directory of Open Access Journals (Sweden)

    Teresa L.F. Ho

    2016-04-01

    Full Text Available Regulation of DNA replication and cell division is essential for tissue growth and maintenance of genomic integrity and is particularly important in tissues that undergo continuous regeneration such as mammary glands. We have previously shown that disruption of the KRAB-domain zinc finger protein Roma/Zfp157 results in hyperproliferation of mammary epithelial cells (MECs during pregnancy. Here, we delineate the mechanism by which Roma engenders this phenotype. Ablation of Roma in MECs leads to unscheduled proliferation, replication stress, DNA damage, and genomic instability. Furthermore, mouse embryonic fibroblasts (MEFs depleted for Roma exhibit downregulation of p21Cip1 and geminin and have accelerated replication fork velocities, which is accompanied by a high rate of mitotic errors and polyploidy. In contrast, overexpression of Roma in MECs halts cell-cycle progression, whereas siRNA-mediated p21Cip1 knockdown ameliorates, in part, this phenotype. Thus, Roma is an essential regulator of the cell cycle and is required to maintain genomic stability.

  8. Stability and Control of Large-Scale Dynamical Systems A Vector Dissipative Systems Approach

    CERN Document Server

    Haddad, Wassim M

    2011-01-01

    Modern complex large-scale dynamical systems exist in virtually every aspect of science and engineering, and are associated with a wide variety of physical, technological, environmental, and social phenomena, including aerospace, power, communications, and network systems, to name just a few. This book develops a general stability analysis and control design framework for nonlinear large-scale interconnected dynamical systems, and presents the most complete treatment on vector Lyapunov function methods, vector dissipativity theory, and decentralized control architectures. Large-scale dynami

  9. Offshore Wind Farms and HVDC Grids Modeling as a Feedback Control System for Stability Analysis

    OpenAIRE

    Bidadfar, Ali; Saborío-Romano, Oscar; Altin, Müfit; Göksu, Ömer; Cutululis, Nicolaos Antonio; Sørensen, Poul Ejnar

    2017-01-01

    The low impedance characteristics of DC transmission lines cause the voltage source converter (VSC) in HVDC networks to become electrically closer together and increase the risk of severe interactions between the converters. Such interactions, in turn, intensify the implementation of the grid control schemes and may lead the entire system to instability. Assessing the stability and adopting complex coordinated control schemes in an HVDC grid and wind farm turbines are challenging and require ...

  10. A Good Turn Deserves Another: Political Stability, Corruption and Corruption-Control

    OpenAIRE

    Simplice A Asongu

    2015-01-01

    We build on existing literature and contemporary challenges to African development to assess the role of political stability in fighting corruption and boosting corruption-control in 53 African countries for the period 1996-2010. We postulate that on the one hand, an atmosphere of political instability should increase the confidence of impunity owing to less corruption-control. On the other hand, in the absence such impunity from corruption, political instability further fuels corruption. Our...

  11. Evidence for widespread degradation of gene control regions in hominid genomes.

    Directory of Open Access Journals (Sweden)

    Peter D Keightley

    2005-02-01

    Full Text Available Although sequences containing regulatory elements located close to protein-coding genes are often only weakly conserved during evolution, comparisons of rodent genomes have implied that these sequences are subject to some selective constraints. Evolutionary conservation is particularly apparent upstream of coding sequences and in first introns, regions that are enriched for regulatory elements. By comparing the human and chimpanzee genomes, we show here that there is almost no evidence for conservation in these regions in hominids. Furthermore, we show that gene expression is diverging more rapidly in hominids than in murids per unit of neutral sequence divergence. By combining data on polymorphism levels in human noncoding DNA and the corresponding human-chimpanzee divergence, we show that the proportion of adaptive substitutions in these regions in hominids is very low. It therefore seems likely that the lack of conservation and increased rate of gene expression divergence are caused by a reduction in the effectiveness of natural selection against deleterious mutations because of the low effective population sizes of hominids. This has resulted in the accumulation of a large number of deleterious mutations in sequences containing gene control elements and hence a widespread degradation of the genome during the evolution of humans and chimpanzees.

  12. Orthogonal control of expression mean and variance by epigenetic features at different genomic loci.

    Science.gov (United States)

    Dey, Siddharth S; Foley, Jonathan E; Limsirichai, Prajit; Schaffer, David V; Arkin, Adam P

    2015-05-05

    While gene expression noise has been shown to drive dramatic phenotypic variations, the molecular basis for this variability in mammalian systems is not well understood. Gene expression has been shown to be regulated by promoter architecture and the associated chromatin environment. However, the exact contribution of these two factors in regulating expression noise has not been explored. Using a dual-reporter lentiviral model system, we deconvolved the influence of the promoter sequence to systematically study the contribution of the chromatin environment at different genomic locations in regulating expression noise. By integrating a large-scale analysis to quantify mRNA levels by smFISH and protein levels by flow cytometry in single cells, we found that mean expression and noise are uncorrelated across genomic locations. Furthermore, we showed that this independence could be explained by the orthogonal control of mean expression by the transcript burst size and noise by the burst frequency. Finally, we showed that genomic locations displaying higher expression noise are associated with more repressed chromatin, thereby indicating the contribution of the chromatin environment in regulating expression noise. © 2015 The Authors. Published under the terms of the CC BY 4.0 license.

  13. Genomics, Endoscopy, and Control of Gastroesophageal Cancers: A PerspectiveSummary

    Directory of Open Access Journals (Sweden)

    Brian J. Reid

    2017-05-01

    Full Text Available In The Cancer Genome Atlas the goals were to define how to treat advanced cancers with targeted therapy. However, the challenges facing cancer interception for early detection and prevention include length bias in which current screening and surveillance approaches frequently miss rapidly progressing cancers that then present at advanced stages in the clinic with symptoms (underdiagnosis. In contrast, many early detection strategies detect benign conditions that may never progress to cancer during a lifetime, and the patient dies of unrelated causes (overdiagnosis. This challenge to cancer interception is believed to be due to the speed at which the neoplasm evolves, called length bias sampling; rapidly progressing cancers are missed by current early detection strategies. In contrast, slowly or non-progressing cancers or their precursors are selectively detected. This has led to the concept of cancer interception, which can be defined as active interception of a biological process that drives cancer development before the patient presents in the clinic with an advanced, symptomatic cancer. The solutions needed to advance strategies for cancer interception require assessing the rate at which the cancer evolves over time and space. This is an essential challenge that needs to be addressed by robust study designs including normal and non-progressing controls when known to be appropriate. Keywords: Barrett's Esophagus, Biomarkers, Chromosome Aberrations, Esophageal Neoplasms, Gastroesophageal Reflux, Genomic Instability, Genomics, Stomach

  14. Controlling chaos in permanent magnet synchronous motor based on finite-time stability theory

    International Nuclear Information System (INIS)

    Du-Qu, Wei; Bo, Zhang

    2009-01-01

    This paper reports that the performance of permanent magnet synchronous motor (PMSM) degrades due to chaos when its systemic parameters fall into a certain area. To control the undesirable chaos in PMSM, a nonlinear controller, which is simple and easy to be constructed, is presented to achieve finite-time chaos control based on the finite-time stability theory. Computer simulation results show that the proposed controller is very effective. The obtained results may help to maintain the industrial servo driven system's security operation. (general)

  15. Quadratic Stabilization of LPV System by an LTI Controller Based on ILMI Algorithm

    Directory of Open Access Journals (Sweden)

    Wei Xie

    2007-01-01

    Full Text Available A linear time-invariant (LTI output feedback controller is designed for a linear parameter-varying (LPV control system to achieve quadratic stability. The LPV system includes immeasurable dependent parameters that are assumed to vary in a polytopic space. To solve this control problem, a heuristic algorithm is proposed in the form of an iterative linear matrix inequality (ILMI formulation. Furthermore, an effective method of setting an initial value of the ILMI algorithm is also proposed to increase the probability of getting an admissible solution for the controller design problem.

  16. Stability Analysis of a Voltage-Based Controller for Robot Manipulators

    Directory of Open Access Journals (Sweden)

    Jorge Orrante-Sakanassi

    2013-01-01

    Full Text Available A voltage-based control scheme for robot manipulators has been presented in recent literature, where feedback linearization is applied in the electrical equations of the DC motors in order to cancel the electrical current terms. However, in this paper we show that this control technique generates a system of the form Ex = Ax + Bu, where E is a singular matrix, that is to say, a generalized state-space system or singular system. This paper introduces a formal stability analysis of the respective system by considering the state-space equation as a singular system. Furthermore, in order to avoid the singularity of the closed-loop system, modified voltage-based control schemes are proposed, whose Lyapunov stability analyses conclude semiglobal asymptotic stability for the set-point control case and uniform boundedness of the solutions and semiglobal convergence of the position, as well as velocity errors for the tracking control case. The proposed control systems are simulated for the tracking and set-point cases using the CICESE Pelican robot driven by DC motors.

  17. Nonlinear Synergetic Governor Controllers for Steam Turbine Generators to Enhance Power System Stability

    Directory of Open Access Journals (Sweden)

    Xingbao Ju

    2017-07-01

    Full Text Available This paper proposes a decentralized nonlinear synergetic governor controller (NSGC for turbine generators to enhance power system stability by using synergetic control theory and the feedback linearization technique. The precise feedback linearization model of a turbine-generator with a steam valve control is obtained, at first, by using a feedback linearization technique. Then based on this model, a manifold is defined as a linear combination of the deviation of the rotor angle, speed deviation, and speed derivative. The control law of the proposed NSGC is deduced and the stability condition of the whole closed-loop system is subsequently analyzed. According to the requirement of the primary frequency regulation, an additional proportional integral (PI controller is designed to dynamically track the steady-state value of the rotor angle. Case studies are undertaken based on a single-machine infinite-bus system and the New England system, respectively. Simulation results show that the proposed NSGC can suppress the power oscillations and improve transient stability more effectively in comparison with the conventional proportional-integral-derivative (PID governor controller. Moreover, the proposed NSGC is robust to the variations of the system operating conditions.

  18. WIND TUNNEL EVALUATION FOR CONTROL TRANSITION FROM ELEVATOR TO STABILATOR OF SMALL UAV

    Directory of Open Access Journals (Sweden)

    ZULHILMY SAHWEE

    2017-06-01

    Full Text Available Faulty control surface actuator in a small Unmanned Aerial Vehicles (sUAV could be overcome with a few techniques. Redundant actuators, analytical redundancy or combination of both are normally used as fault accommodation techniques. In this paper, the accommodation technique of faulty elevator actuator is presented. This technique uses a standby control surface as temporary control reallocation. Wind tunnel measurement facility is set up for the experimental validation and it is compared with FoilSim software. Flat plate airfoil which was used as horizontal stabilizer, is simulated using numerical model and it is validated using the wind tunnel test. Then, a flat airfoil is designed to be used as stabilator for the recovery of faulty elevator actuator. Results show the different deflection angle is needed when transferring from one control surface to another. From the analysis, the proposed method could be implemented without affecting the pitch stability during control surface transition. The alternate control surface accommodation technique proves to be promising for higher reliability sUAV in the case of a faulty on-board actuator.

  19. Stability Analysis of a Type of Takagi-Sugeno PI Fuzzy Control Systems Using Circle Criterion

    Directory of Open Access Journals (Sweden)

    Kairui Cao

    2011-04-01

    Full Text Available A type of Takagi-Sugeno (T-S Proportional-Integral (PI fuzzy controllers is studied. The T-S PI fuzzy controller is formed by a T-S Proportional-Derivative (PD fuzzy controller connected with an integrator. In this particular structure, the T-S PD fuzzy controller is a weighted sum of some linear PD sub-controllers. The mathematical properties of our T-S PI fuzzy controller are also investigated. Based on these properties, the global asymptotic stability of the fuzzy control systems, in which the T-S PI fuzzy controllers are employed, are analyzed by using the well-known circle criterion. A sufficient condition with an elegant graphical interpretation in the frequency domain is further derived to guarantee the global asymptotic stability of the above fuzzy control systems. Finally, two numerical examples are provided to demonstrate how to deploy this method in analyzing the T-S PI fuzzy control systems in the frequency domain with the aid of some simple graphs.

  20. Stability Constrained Efficiency Optimization for Droop Controlled DC-DC Conversion System

    DEFF Research Database (Denmark)

    Meng, Lexuan; Dragicevic, Tomislav; Guerrero, Josep M.

    2013-01-01

    implementing tertiary regulation. Moreover, system dynamic is affected when shifting VRs. Therefore, the stability is considered in optimization by constraining the eigenvalues arising from dynamic state space model of the system. Genetic algorithm is used in searching for global efficiency optimum while....... As the efficiency of each converter changes with output power, virtual resistances (VRs) are set as decision variables for adjusting power sharing proportion among converters. It is noteworthy that apart from restoring the voltage deviation, secondary control plays an important role to stabilize dc bus voltage when...

  1. Classical linear-control analysis applied to business-cycle dynamics and stability

    Science.gov (United States)

    Wingrove, R. C.

    1983-01-01

    Linear control analysis is applied as an aid in understanding the fluctuations of business cycles in the past, and to examine monetary policies that might improve stabilization. The analysis shows how different policies change the frequency and damping of the economic system dynamics, and how they modify the amplitude of the fluctuations that are caused by random disturbances. Examples are used to show how policy feedbacks and policy lags can be incorporated, and how different monetary strategies for stabilization can be analytically compared. Representative numerical results are used to illustrate the main points.

  2. Exponential stabilization and synchronization for fuzzy model of memristive neural networks by periodically intermittent control.

    Science.gov (United States)

    Yang, Shiju; Li, Chuandong; Huang, Tingwen

    2016-03-01

    The problem of exponential stabilization and synchronization for fuzzy model of memristive neural networks (MNNs) is investigated by using periodically intermittent control in this paper. Based on the knowledge of memristor and recurrent neural network, the model of MNNs is formulated. Some novel and useful stabilization criteria and synchronization conditions are then derived by using the Lyapunov functional and differential inequality techniques. It is worth noting that the methods used in this paper are also applied to fuzzy model for complex networks and general neural networks. Numerical simulations are also provided to verify the effectiveness of theoretical results. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Stabilizing periodic orbits of chaotic systems using fuzzy adaptive sliding mode control

    Energy Technology Data Exchange (ETDEWEB)

    Layeghi, Hamed [Department of Mechanical Engineering, Sharif University of Technology, Center of Excellence in Design, Robotics and Automation, Azadi Avenue, Postal Code 11365-9567 Tehran (Iran, Islamic Republic of)], E-mail: layeghi@mech.sharif.edu; Arjmand, Mehdi Tabe [Department of Mechanical Engineering, Sharif University of Technology, Center of Excellence in Design, Robotics and Automation, Azadi Avenue, Postal Code 11365-9567 Tehran (Iran, Islamic Republic of)], E-mail: arjmand@mech.sharif.edu; Salarieh, Hassan [Department of Mechanical Engineering, Sharif University of Technology, Center of Excellence in Design, Robotics and Automation, Azadi Avenue, Postal Code 11365-9567 Tehran (Iran, Islamic Republic of)], E-mail: salarieh@mech.sharif.edu; Alasty, Aria [Department of Mechanical Engineering, Sharif University of Technology, Center of Excellence in Design, Robotics and Automation, Azadi Avenue, Postal Code 11365-9567 Tehran (Iran, Islamic Republic of)], E-mail: aalasti@sharif.edu

    2008-08-15

    In this paper by using a combination of fuzzy identification and the sliding mode control a fuzzy adaptive sliding mode scheme is designed to stabilize the unstable periodic orbits of chaotic systems. The chaotic system is assumed to have an affine form x{sup (n)} = f(X) + g(X)u where f and g are unknown functions. Using only the input-output data obtained from the underlying dynamical system, two fuzzy systems are constructed for identification of f and g. Two distinct methods are utilized for fuzzy modeling, the least squares and the gradient descent techniques. Based on the estimated fuzzy models, an adaptive controller, which works through the sliding mode control, is designed to make the system track the desired unstable periodic orbits. The stability analysis of the overall closed loop system is presented in the paper and the effectiveness of the proposed adaptive scheme is numerically investigated. As a case of study, modified Duffing system is selected for applying the proposed method to stabilize its 2{pi} and 4{pi} periodic orbits. Simulation results show the high performance of the method for stabilizing the unstable periodic orbits of unknown chaotic systems.

  4. Genome-Wide Association Identifies Multiple Genomic Regions Associated with Susceptibility to and Control of Ovine Lentivirus

    Science.gov (United States)

    2012-10-17

    to varying degrees of dyspnea (respiratory distress), cachexia (body condition wasting), mastitis , arthritis, and/or encephalitis [5,6]. One of the...General Transcription Factor IIH, polypeptide 5), the gene order does not agree with other mammal genomes including cow , human, dog, and mouse, and it may

  5. Design of a Stability Augmentation System for an Unmanned Helicopter Based on Adaptive Control Techniques

    Directory of Open Access Journals (Sweden)

    Shouzhao Sheng

    2015-09-01

    Full Text Available The task of control of unmanned helicopters is rather complicated in the presence of parametric uncertainties and measurement noises. This paper presents an adaptive model feedback control algorithm for an unmanned helicopter stability augmentation system. The proposed algorithm can achieve a guaranteed model reference tracking performance and speed up the convergence rates of adjustable parameters, even when the plant parameters vary rapidly. Moreover, the model feedback strategy in the algorithm further contributes to the improvement in the control quality of the stability augmentation system in the case of low signal to noise ratios, mainly because the model feedback path is noise free. The effectiveness and superiority of the proposed algorithm are demonstrated through a series of tests.

  6. Implementation of model predictive control for resistive wall mode stabilization on EXTRAP T2R

    Science.gov (United States)

    Setiadi, A. C.; Brunsell, P. R.; Frassinetti, L.

    2015-10-01

    A model predictive control (MPC) method for stabilization of the resistive wall mode (RWM) in the EXTRAP T2R reversed-field pinch is presented. The system identification technique is used to obtain a linearized empirical model of EXTRAP T2R. MPC employs the model for prediction and computes optimal control inputs that satisfy performance criterion. The use of a linearized form of the model allows for compact formulation of MPC, implemented on a millisecond timescale, that can be used for real-time control. The design allows the user to arbitrarily suppress any selected Fourier mode. The experimental results from EXTRAP T2R show that the designed and implemented MPC successfully stabilizes the RWM.

  7. Implementation of model predictive control for resistive wall mode stabilization on EXTRAP T2R

    International Nuclear Information System (INIS)

    Setiadi, A C; Brunsell, P R; Frassinetti, L

    2015-01-01

    A model predictive control (MPC) method for stabilization of the resistive wall mode (RWM) in the EXTRAP T2R reversed-field pinch is presented. The system identification technique is used to obtain a linearized empirical model of EXTRAP T2R. MPC employs the model for prediction and computes optimal control inputs that satisfy performance criterion. The use of a linearized form of the model allows for compact formulation of MPC, implemented on a millisecond timescale, that can be used for real-time control. The design allows the user to arbitrarily suppress any selected Fourier mode. The experimental results from EXTRAP T2R show that the designed and implemented MPC successfully stabilizes the RWM. (paper)

  8. Inverse Doppler shift and control field as coherence generators for the stability in superluminal light

    Science.gov (United States)

    Ghafoor, Fazal; Bacha, Bakht Amin; Khan, Salman

    2015-05-01

    A gain-based four-level atomic medium for the stability in superluminal light propagation using control field and inverse Doppler shift as coherence generators is studied. In regimes of weak and strong control field, a broadband and multiple controllable transparency windows are, respectively, identified with significantly enhanced group indices. The observed Doppler effect for the class of high atomic velocity of the medium is counterintuitive in comparison to the effect of the class of low atomic velocity. The intensity of each of the two pump fields is kept less than the optimum limit reported in [M. D. Stenner and D. J. Gauthier, Phys. Rev. A 67, 063801 (2003), 10.1103/PhysRevA.67.063801] for stability in the superluminal light pulse. Consequently, superluminal stable domains with the generated coherence are explored.

  9. Stabilization of Continuous-Time Random Switching Systems via a Fault-Tolerant Controller

    Directory of Open Access Journals (Sweden)

    Guoliang Wang

    2017-01-01

    Full Text Available This paper focuses on the stabilization problem of continuous-time random switching systems via exploiting a fault-tolerant controller, where the dwell time of each subsystem consists of a fixed part and random part. It is known from the traditional design methods that the computational complexity of LMIs related to the quantity of fault combination is very large; particularly system dimension or amount of subsystems is large. In order to reduce the number of the used fault combinations, new sufficient LMI conditions for designing such a controller are established by a robust approach, which are fault-free and could be solved directly. Moreover, the fault-tolerant stabilization realized by a mode-independent controller is considered and suitably applied to a practical case without mode information. Finally, a numerical example is used to demonstrate the effectiveness and superiority of the proposed methods.

  10. Solving ill-posed control problems by stabilized finite element methods: an alternative to Tikhonov regularization

    Science.gov (United States)

    Burman, Erik; Hansbo, Peter; Larson, Mats G.

    2018-03-01

    Tikhonov regularization is one of the most commonly used methods for the regularization of ill-posed problems. In the setting of finite element solutions of elliptic partial differential control problems, Tikhonov regularization amounts to adding suitably weighted least squares terms of the control variable, or derivatives thereof, to the Lagrangian determining the optimality system. In this note we show that the stabilization methods for discretely ill-posed problems developed in the setting of convection-dominated convection-diffusion problems, can be highly suitable for stabilizing optimal control problems, and that Tikhonov regularization will lead to less accurate discrete solutions. We consider some inverse problems for Poisson’s equation as an illustration and derive new error estimates both for the reconstruction of the solution from the measured data and reconstruction of the source term from the measured data. These estimates include both the effect of the discretization error and error in the measurements.

  11. Virtual velocity loop based on MEMS accelerometers for optical stabilization control system

    Science.gov (United States)

    Ren, Wei; Deng, Chao; Mao, Yao; Ren, Ge

    2017-08-01

    In the optical stabilization control system (OSCS) control system based on a charge-coupled device (CCD), stabilization performance of the line-of-sight is severely limited by the mechanical resonance and the low sampling rate of the CCD. An approach to improve the stabilization performance of the OSCS control system with load restriction based on three loops, including an acceleration loop, a virtual velocity loop, and a position loop, by using MEMS accelerometers and a CCD is proposed. The velocity signal is obtained by accelerators instead of gyro sensors. Its advantages are low power, low cost, small size, and wide measuring range. A detailed analysis is provided to show how to design the virtual velocity loop and correct virtual velocity loop drift. Experimental results show that the proposed multiloop feedback control method with virtual velocity loop in which the disturbance suppression performance is better than that of the dual loop control with only an acceleration loop and a position loop at low frequency.

  12. Design of stability-guaranteed fuzzy logic controller for nuclear steam generators

    International Nuclear Information System (INIS)

    Cho, B.H.; No, H.C.

    1996-01-01

    A fuzzy logic controller (FLC) and a fuzzy logic filter (FLF), which have a special type of fuzzifier, inference engine, and defuzzifier, are applied to the water level control of a nuclear steam generator (S/G). It is shown that arbitrary two-input, single-output linear controllers can be adequately expressed by this FLC. A procedure to construct stability-guaranteed FLC rules is proposed. It contains the following steps: (1) the stable sector of linear feedback gains is obtained from the suboptimal concept based on LQR theory and the Lyapunov's stability criteria; (2) the stable sector of linear gains is mapped into two linear rule tables that are used as limits for the FLC rules; and (3) the construction of an FLC rule table is done by choosing certain rules that lie between these limits. This type of FLC guarantees asymptotic stability of the control system. The FLF generates a feedforward signal of S/G feedwater from the steam flow measurement using a fuzzy concept. Through computer simulation, it is found that the FLC with the FLF works better than a well-tuned PID controller with variable gains to reduce swell/shrink phenomena, especially for the water level deviation and abrupt steam flow disturbances that are typical in the existing power plants

  13. Dc microgrid stabilization through fuzzy control of interleaved, heterogeneous storage elements

    Science.gov (United States)

    Smith, Robert David

    As microgrid power systems gain prevalence and renewable energy comprises greater and greater portions of distributed generation, energy storage becomes important to offset the higher variance of renewable energy sources and maximize their usefulness. One of the emerging techniques is to utilize a combination of lead-acid batteries and ultracapacitors to provide both short and long-term stabilization to microgrid systems. The different energy and power characteristics of batteries and ultracapacitors imply that they ought to be utilized in different ways. Traditional linear controls can use these energy storage systems to stabilize a power grid, but cannot effect more complex interactions. This research explores a fuzzy logic approach to microgrid stabilization. The ability of a fuzzy logic controller to regulate a dc bus in the presence of source and load fluctuations, in a manner comparable to traditional linear control systems, is explored and demonstrated. Furthermore, the expanded capabilities (such as storage balancing, self-protection, and battery optimization) of a fuzzy logic system over a traditional linear control system are shown. System simulation results are presented and validated through hardware-based experiments. These experiments confirm the capabilities of the fuzzy logic control system to regulate bus voltage, balance storage elements, optimize battery usage, and effect self-protection.

  14. Extension and customization of self-stability control in compliant legged systems

    International Nuclear Information System (INIS)

    Ernst, M; Blickhan, R; Geyer, H

    2012-01-01

    Several recent studies on the control of legged locomotion in animal and robot running focus on the influence of different leg parameters on gait stability. In a preceding investigation self-stability controls showing deadbeat behavior could be obtained by studying the dynamics of the system in dependence of the leg orientation carefully adjusted during the flight phase. Such controls allow to accommodate disturbances of the ground level without having to detect them. Here we further this method in two ways. Besides the leg orientation, we allow changes in leg stiffness during flight and show that this extension substantially improves the rejection of ground disturbances. In a human like example the tolerance of random variation in ground level over many steps increased from 3.5% to 35% of leg length. In single steps changes of about 70% leg length (either up or down) could be negotiated. The variable leg stiffness not only allows to start with flat leg orientations maximizing step tolerances but also increase the control subspace. This allows to customize self-stability controls and to consider physical and technical limitations found in animals and robots. (paper)

  15. Fuzzy Logic Controller Stability Analysis Using a Satisfiability Modulo Theories Approach

    Science.gov (United States)

    Arnett, Timothy; Cook, Brandon; Clark, Matthew A.; Rattan, Kuldip

    2017-01-01

    While many widely accepted methods and techniques exist for validation and verification of traditional controllers, at this time no solutions have been accepted for Fuzzy Logic Controllers (FLCs). Due to the highly nonlinear nature of such systems, and the fact that developing a valid FLC does not require a mathematical model of the system, it is quite difficult to use conventional techniques to prove controller stability. Since safety-critical systems must be tested and verified to work as expected for all possible circumstances, the fact that FLC controllers cannot be tested to achieve such requirements poses limitations on the applications for such technology. Therefore, alternative methods for verification and validation of FLCs needs to be explored. In this study, a novel approach using formal verification methods to ensure the stability of a FLC is proposed. Main research challenges include specification of requirements for a complex system, conversion of a traditional FLC to a piecewise polynomial representation, and using a formal verification tool in a nonlinear solution space. Using the proposed architecture, the Fuzzy Logic Controller was found to always generate negative feedback, but inconclusive for Lyapunov stability.

  16. Improved Robust Stability Criterion of Networked Control Systems with Transmission Delays and Packet Loss

    Directory of Open Access Journals (Sweden)

    Shenping Xiao

    2014-01-01

    Full Text Available The problem of stability analysis for a class of networked control systems (NCSs with network-induced delay and packet dropout is investigated in this paper. Based on the working mechanism of zero-order holder, the closed-loop NCS is modeled as a continuous-time linear system with input delay. By introducing a novel Lyapunov-Krasovskii functional which splits both the lower and upper bounds of the delay into two subintervals, respectively, and utilizes reciprocally convex combination technique, a new stability criterion is derived in terms of linear matrix inequalities. Compared with previous results in the literature, the obtained stability criterion is less conservative. Numerical examples demonstrate the validity and feasibility of the proposed method.

  17. Partial stabilization and control of distributed parameter systems with elastic elements

    CERN Document Server

    Zuyev, Alexander L

    2015-01-01

     This monograph provides a rigorous treatment of problems related to partial asymptotic stability and controllability for models of flexible structures described by coupled nonlinear ordinary and partial differential equations or equations in abstract spaces. The text is self-contained, beginning with some basic results from the theory of continuous semigroups of operators in Banach spaces. The problem of partial asymptotic stability with respect to a continuous functional is then considered for a class of abstract multivalued systems on a metric space. Next, the results of this study are applied to the study of a rotating body with elastic attachments. Professor Zuyev demonstrates that the equilibrium cannot be made strongly asymptotically stable in the general case, motivating consideration of the problem of partial stabilization with respect to the functional that represents “averaged” oscillations. The book’s focus moves on to spillover analysis for infinite-dimensional systems with finite-dimensio...

  18. Frequency stabilization of a He-Ne gas laser by controlling refractive index of laser plasma

    International Nuclear Information System (INIS)

    Xie Yi; Wu Yizun

    1991-01-01

    A new way to stabilize the frequency of a Zeeman He-Ne gas laser is described. The laser frequency is stabilized by controlling the refractive index of the laser plasma. It does not need a gas laser tube with a piezoelectric ceramic (PZT) made by special technology. As the phase-locking technology is used in the laser servo system, the self-beat frequency is a constant and the frequency stability is better than 2.2 x 10 -11 (averaging time = 10 sec.). The long term frequency fluctuation never exceeded 2 x 10 -8 during two months. The frequency of the locked point can be adjusted continuously in the range of over 200 MHz

  19. Anfis Approach for Sssc Controller Design for the Improvement of Transient Stability Performance

    Science.gov (United States)

    Khuntia, Swasti R.; Panda, Sidhartha

    2011-06-01

    In this paper, Adaptive Neuro-Fuzzy Inference System (ANFIS) method based on the Artificial Neural Network (ANN) is applied to design a Static Synchronous Series Compensator (SSSC)-based controller for improvement of transient stability. The proposed ANFIS controller combines the advantages of fuzzy controller and quick response and adaptability nature of ANN. The ANFIS structures were trained using the generated database by fuzzy controller of SSSC. It is observed that the proposed SSSC controller improves greatly the voltage profile of the system under severe disturbances. The results prove that the proposed SSSC-based ANFIS controller is found to be robust to fault location and change in operating conditions. Further, the results obtained are compared with the conventional lead-lag controllers for SSSC.

  20. Stabilization and control of quad-rotor helicopter using a smartphone device

    Science.gov (United States)

    Desai, Alok; Lee, Dah-Jye; Moore, Jason; Chang, Yung-Ping

    2013-01-01

    In recent years, autonomous, micro-unmanned aerial vehicles (micro-UAVs), or more specifically hovering micro- UAVs, have proven suitable for many promising applications such as unknown environment exploration and search and rescue operations. The early versions of UAVs had no on-board control capabilities, and were difficult for manual control from a ground station. Many UAVs now are equipped with on-board control systems that reduce the amount of control required from the ground-station operator. However, the limitations on payload, power consumption and control without human interference remain the biggest challenges. This paper proposes to use a smartphone as the sole computational device to stabilize and control a quad-rotor. The goal is to use the readily available sensors in a smartphone such as the GPS, the accelerometer, the rate-gyros, and the camera to support vision-related tasks such as flight stabilization, estimation of the height above ground, target tracking, obstacle detection, and surveillance. We use a quad-rotor platform that has been built in the Robotic Vision Lab at Brigham Young University for our development and experiments. An Android smartphone is connected through the USB port to an external hardware that has a microprocessor and circuitries to generate pulse-width modulation signals to control the brushless servomotors on the quad-rotor. The high-resolution camera on the smartphone is used to detect and track features to maintain a desired altitude level. The vision algorithms implemented include template matching, Harris feature detector, RANSAC similarity-constrained homography, and color segmentation. Other sensors are used to control yaw, pitch, and roll of the quad-rotor. This smartphone-based system is able to stabilize and control micro-UAVs and is ideal for micro-UAVs that have size, weight, and power limitations.

  1. A Lyapunov Stability Theory-Based Control Strategy for Three-Level Shunt Active Power Filter

    Directory of Open Access Journals (Sweden)

    Yijia Cao

    2017-01-01

    Full Text Available The three-phase three-wire neutral-point-clamped shunt active power filter (NPC-SAPF, which most adopts classical closed-loop feedback control methods such as proportional-integral (PI, proportional-resonant (PR and repetitive control, can only output 1st–25th harmonic currents with 10–20 kHz switching frequency. The reason for this is that the controller design must make a compromise between system stability and harmonic current compensation ability under the condition of less than 20 kHz switching frequency. To broaden the bandwidth of the compensation current, a Lyapunov stability theory-based control strategy is presented in this paper for NPC-SAPF. The proposed control law is obtained by constructing the switching function on the basis of the mathematical model and the Lyapunov candidate function, which can avoid introducing closed-loop feedback control and keep the system globally asymptotically stable. By means of the proposed method, the NPC-SAPF has compensation ability for the 1st–50th harmonic currents, the total harmonic distortion (THD and each harmonic content of grid currents satisfy the requirements of IEEE Standard 519-2014. In order to verify the superiority of the proposed control strategy, stability conditions of the proposed strategy and the representative PR controllers are compared. The simulation results in MATLAB/Simulink (MathWorks, Natick, MA, USA and the experimental results obtained on a 6.6 kVA NPC-SAPF laboratory prototype validate the proposed control strategy.

  2. Stabilization of a semilinear parabolic equation in the exterior of a bounded domain by means of boundary controls

    International Nuclear Information System (INIS)

    Gorshkov, A V

    2003-01-01

    The problem of the stabilization of a semilinear equation in the exterior of a bounded domain is considered. In view of the impossibility of an exponential stabilization of the form e -σt of the solution of a parabolic equation in an unbounded domain no matter what the boundary control is, one poses the problem of power-like stabilization by means of a boundary control. For a fixed initial condition and parameter k>0 of the rate of stabilization the existence of a boundary control such that the solution approaches zero at the rate 1/t k is demonstrated

  3. Coordinated Control of Multiterminal DC Grid Power Injections for Improved Rotor-Angle Stability Based on Lyapunov Theory

    DEFF Research Database (Denmark)

    Eriksson, Robert

    2014-01-01

    The stability of an interconnected ac/dc system is affected by disturbances occurring in the system. Disturbances, such as three-phase faults, may jeopardize the rotor-angle stability and, thus, the generators fall out of synchronism. The possibility of fast change of the injected powers...... by the multiterminal dc grid can, by proper control action, enhance this stability. This paper proposes a new time optimal control strategy for the injected power of multiterminal dc grids to enhance the rotor-angle stability. The controller is time optimal, since it reduces the impact of a disturbance as fast...

  4. The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress

    DEFF Research Database (Denmark)

    Clausing, Emanuel; Mayer, Andreas; Chanarat, Sittinan

    2010-01-01

    Multiple DNA-associated processes such as DNA repair, replication, and recombination are crucial for the maintenance of genome integrity. Here, we show a novel interaction between the transcription elongation factor Bur1-Bur2 and replication protein A (RPA), the eukaryotic single-stranded DNA......-binding protein with functions in DNA repair, recombination, and replication. Bur1 interacted via its C-terminal domain with RPA, and bur1-¿C mutants showed a deregulated DNA damage response accompanied by increased sensitivity to DNA damage and replication stress as well as increased levels of persisting Rad52...... foci. Interestingly, the DNA damage sensitivity of an rfa1 mutant was suppressed by bur1 mutation, further underscoring a functional link between these two protein complexes. The transcription elongation factor Bur1-Bur2 interacts with RPA and maintains genome integrity during DNA replication stress....

  5. Stabilization of the genome of the mismatch repair deficient Mycobacterium tuberculosis by context-dependent codon choice

    OpenAIRE

    Wanner, Roger M; Güthlein, Carolin; Springer, Burkhard; Böttger, Erik C; Ackermann, Martin

    2008-01-01

    Abstract Background The rate at which a stretch of DNA mutates is determined by the cellular systems for DNA replication and repair, and by the nucleotide sequence of the stretch itself. One sequence feature with a particularly strong influence on the mutation rate are nucleotide repeats. Some microbial pathogens use nucleotide repeats in their genome to stochastically vary phenotypic traits and thereby evade host defense. However, such unstable sequences also come at a cost, as mutations are...

  6. The origin, evolution and proposed stabilization of the terms "genome size' and 'C-value' to describe nuclear DNA contents

    Czech Academy of Sciences Publication Activity Database

    Greilhuber, J.; Doležel, Jaroslav; Lysák, Martin; Bennett, M. D.

    2005-01-01

    Roč. 95, č. 1 (2005), s. 255-260 ISSN 0305-7364 R&D Pro jects: GA ČR GA522/03/0354 Grant - others:Austrian Science Fund(AT) P14607-B03 Institutional research plan: CEZ:AV0Z50380511 Keywords : genome size * C- value * Cx- value Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.665, year: 2005

  7. Flux stability and power control in the Soviet RBMK-1000 reactors

    International Nuclear Information System (INIS)

    Meriwether, G.H.; McNeece, J.P.

    1993-08-01

    As a result of the Chernobyl accident, the Soviets have studied and implemented various design changes to improve the safety of the RBMK reactors. The safety measurements include modifications of the control rod configuration, fuel enrichment increase from 2.0 to 2.4 weight percent U-235, and installation of additional supplemental absorbers. The purpose of this study is to investigate the effects of increased fuel enrichment, different control rod positions, and supplemental absorber loadings on reactivity control, power distribution within the large RBMK core, and relative stability against power oscillations

  8. Thermal stability control system of photo-elastic interferometer in the PEM-FTs

    Science.gov (United States)

    Zhang, M. J.; Jing, N.; Li, K. W.; Wang, Z. B.

    2018-01-01

    A drifting model for the resonant frequency and retardation amplitude of a photo-elastic modulator (PEM) in the photo-elastic modulated Fourier transform spectrometer (PEM-FTs) is presented. A multi-parameter broadband-matching driving control method is proposed to improve the thermal stability of the PEM interferometer. The automatically frequency-modulated technology of the driving signal based on digital phase-locked technology is used to track the PEM's changing resonant frequency. Simultaneously the maximum optical-path-difference of a laser's interferogram is measured to adjust the amplitude of the PEM's driving signal so that the spectral resolution is stable. In the experiment, the multi-parameter broadband-matching control method is applied to the driving control system of the PEM-FTs. Control of resonant frequency and retardation amplitude stabilizes the maximum optical-path-difference to approximately 236 μm and results in a spectral resolution of 42 cm-1. This corresponds to a relative error smaller than 2.16% (4.28 standard deviation). The experiment shows that the method can effectively stabilize the spectral resolution of the PEM-FTs.

  9. Validation of enhanced stabilization of municipal solid waste under controlled leachate recirculation using FTIR and XRD.

    Science.gov (United States)

    Sethi, Sapna; Kothiyal, N C; Nema, Arvind K

    2012-07-01

    Leachate recirculation at neutral PH accompanied with buffer/nutrients addition has been used successfully in earlier stabilization of municipal solid waste in bioreactor landfills. In the present study, efforts were made to enhance the stabilization rate of municipal solid waste (MSW) and organic solid waste (OSW) in simulated landfill bioreactors by controlling the pH of recirculated leachate towards slightly alkaline side in absence of additional buffer and nutrients addition. Enhanced stabilization in waste samples was monitored with the help of analytical tools like Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD). Predominance of bands assigned to inorganic compounds and comparatively lower intensities of bands for organic compounds in the FTIR spectra of waste samples degraded with leachate recirculation under controlled pH confirmed higher rate of biodegradation and mineralization of waste than the samples degraded without controlled leachate recirculation. XRD spectra also confirmed to a greater extent of mineralization in the waste samples degraded under leachate recirculation with controlled pH. Comparison of XRD spectra of two types of wastes pointed out higher degree of mineralization in organic solid waste as compared to municipal solid waste.

  10. QTL mapping of genome regions controlling temephos resistance in larvae of the mosquito Aedes aegypti.

    Science.gov (United States)

    Reyes-Solis, Guadalupe Del Carmen; Saavedra-Rodriguez, Karla; Suarez, Adriana Flores; Black, William C

    2014-10-01

    The mosquito Aedes aegypti is the principal vector of dengue and yellow fever flaviviruses. Temephos is an organophosphate insecticide used globally to suppress Ae. aegypti larval populations but resistance has evolved in many locations. Quantitative Trait Loci (QTL) controlling temephos survival in Ae. aegypti larvae were mapped in a pair of F3 advanced intercross lines arising from temephos resistant parents from Solidaridad, México and temephos susceptible parents from Iquitos, Peru. Two sets of 200 F3 larvae were exposed to a discriminating dose of temephos and then dead larvae were collected and preserved for DNA isolation every two hours up to 16 hours. Larvae surviving longer than 16 hours were considered resistant. For QTL mapping, single nucleotide polymorphisms (SNPs) were identified at 23 single copy genes and 26 microsatellite loci of known physical positions in the Ae. aegypti genome. In both reciprocal crosses, Multiple Interval Mapping identified eleven QTL associated with time until death. In the Solidaridad×Iquitos (SLD×Iq) cross twelve were associated with survival but in the reciprocal IqxSLD cross, only six QTL were survival associated. Polymorphisms at acetylcholine esterase (AchE) loci 1 and 2 were not associated with either resistance phenotype suggesting that target site insensitivity is not an organophosphate resistance mechanism in this region of México. Temephos resistance is under the control of many metabolic genes of small effect and dispersed throughout the Ae. aegypti genome.

  11. Genomic imprinting, growth control and the allocation of nutritional resources: consequences for postnatal life.

    Science.gov (United States)

    Charalambous, Marika; da Rocha, Simão Teixeira; Ferguson-Smith, Anne C

    2007-02-01

    Genes subject to genomic imprinting are predominantly expressed from one of the two parental chromosomes, are often clustered in the genome, and their activity and repression are epigenetically regulated. The role of imprinted genes in growth control has been apparent since the discovery of imprinting in the early 1980s. Drawing from studies in the mouse, we propose three distinct classes of imprinted genes - those expressed, imprinted and acting predominantly within the placenta, those with no associated foetal growth effects that act postnatally to regulate metabolic processes, and those expressed in the embryo and placenta that programme the development of organs participating in metabolic processes. Members of this latter class may interact in functional networks regulating the interaction between the mother and the foetus, affecting generalized foetal well-being, growth and organ development; they may also coordinately regulate the development of particular organ systems. The mono-allelic behaviour and sensitivity to changes in regional epigenetic states renders imprinted genes adaptable and vulnerable; in all cases, their perturbed dosage can compromise prenatal and/or postnatal control of nutritional resources. This finding has implications for understanding the relationships between prenatal events and diseases later in life.

  12. A Control Framework for Anthropomorphic Biped Walking Based on Stabilizing Feedforward Trajectories.

    Science.gov (United States)

    Rezazadeh, Siavash; Gregg, Robert D

    2016-10-01

    Although dynamic walking methods have had notable successes in control of bipedal robots in the recent years, still most of the humanoid robots rely on quasi-static Zero Moment Point controllers. This work is an attempt to design a highly stable controller for dynamic walking of a human-like model which can be used both for control of humanoid robots and prosthetic legs. The method is based on using time-based trajectories that can induce a highly stable limit cycle to the bipedal robot. The time-based nature of the controller motivates its use to entrain a model of an amputee walking, which can potentially lead to a better coordination of the interaction between the prosthesis and the human. The simulations demonstrate the stability of the controller and its robustness against external perturbations.

  13. Real time computer control of a nonlinear Multivariable System via Linearization and Stability Analysis

    International Nuclear Information System (INIS)

    Raza, K.S.M.

    2004-01-01

    This paper demonstrates that if a complicated nonlinear, non-square, state-coupled multi variable system is smartly linearized and subjected to a thorough stability analysis then we can achieve our design objectives via a controller which will be quite simple (in term of resource usage and execution time) and very efficient (in terms of robustness). Further the aim is to implement this controller via computer in a real time environment. Therefore first a nonlinear mathematical model of the system is achieved. An intelligent work is done to decouple the multivariable system. Linearization and stability analysis techniques are employed for the development of a linearized and mathematically sound control law. Nonlinearities like the saturation in actuators are also been catered. The controller is then discretized using Runge-Kutta integration. Finally the discretized control law is programmed in a computer in a real time environment. The programme is done in RT -Linux using GNU C for the real time realization of the control scheme. The real time processes, like sampling and controlled actuation, and the non real time processes, like graphical user interface and display, are programmed as different tasks. The issue of inter process communication, between real time and non real time task is addressed quite carefully. The results of this research pursuit are presented graphically. (author)

  14. Stability Analysis and Optimal Control Strategy for Prevention of Pine Wilt Disease

    Directory of Open Access Journals (Sweden)

    Kwang Sung Lee

    2014-01-01

    Full Text Available We propose a mathematical model of pine wilt disease (PWD which is caused by pine sawyer beetles carrying the pinewood nematode (PWN. We calculate the basic reproduction number R0 and investigate the stability of a disease-free and endemic equilibrium in a given mathematical model. We show that the stability of the equilibrium in the proposed model can be controlled through the basic reproduction number R0. We then discuss effective optimal control strategies for the proposed PWD mathematical model. We demonstrate the existence of a control problem, and then we apply both analytical and numerical techniques to demonstrate effective control methods to prevent the transmission of the PWD. In order to do this, we apply two control strategies: tree-injection of nematicide and the eradication of adult beetles through aerial pesticide spraying. Optimal prevention strategies can be determined by solving the corresponding optimality system. Numerical simulations of the optimal control problem using a set of reasonable parameter values suggest that reducing the number of pine sawyer beetles is more effective than the tree-injection strategy for controlling the spread of PWD.

  15. Tutorial on Feedback Control of Flows, Part I: Stabilization of Fluid Flows in Channels and Pipes

    Directory of Open Access Journals (Sweden)

    Ole M. Aamo

    2002-07-01

    Full Text Available The field of flow control has picked up pace over the past decade or so, on the promise of real-time distributed control on turbulent scales being realizable in the near future. This promise is due to the micromachining technology that emerged in the 1980s and developed at an amazing speed through the 1990s. In lab experiments, so called micro-electro-mechanical systems (MEMS that incorporate the entire detection-decision-actuation process on a single chip, have been batch processed in large numbers and assembled into flexible skins for gluing onto body-fluid interfaces for drag reduction purposes. Control of fluid flows span a wide variety of specialities. In Part I of this tutorial, we focus on the problem of reducing drag in channel and pipe flows by stabilizing the parabolic equilibrium profile using boundary feedback control. The control strategics used for this problem include classical control, based on the Nyquist criteria, and various optimal control techniques (H2, H-Infinity, as well as applications of Lyapunov stability theory.

  16. The characterization of twenty sequenced human genomes.

    Directory of Open Access Journals (Sweden)

    Kimberly Pelak

    2010-09-01

    Full Text Available We present the analysis of twenty human genomes to evaluate the prospects for identifying rare functional variants that contribute to a phenotype of interest. We sequenced at high coverage ten "case" genomes from individuals with severe hemophilia A and ten "control" genomes. We summarize the number of genetic variants emerging from a study of this magnitude, and provide a proof of concept for the identification of rare and highly-penetrant functional variants by confirming that the cause of hemophilia A is easily recognizable in this data set. We also show that the number of novel single nucleotide variants (SNVs discovered per genome seems to stabilize at about 144,000 new variants per genome, after the first 15 individuals have been sequenced. Finally, we find that, on average, each genome carries 165 homozygous protein-truncating or stop loss variants in genes representing a diverse set of pathways.

  17. On genome-wide association studies for family-based designs: an integrative analysis approach combining ascertained family samples with unselected controls.

    NARCIS (Netherlands)

    Lasky-Su, J.; Won, S.; Mick, E.; Anney, R.J.; Franke, B.; Neale, B.; Biederman, J.; Smalley, S.L.; Loo, S.K.; Todorov, A.A.; Faraone, S.V.; Weiss, S.T.; Lange, C.

    2010-01-01

    Large numbers of control individuals with genome-wide genotype data are now available through various databases. These controls are regularly used in case-control genome-wide association studies (GWAS) to increase the statistical power. Controls are often "unselected" for the disease of interest and

  18. Control of magnetohydrodynamic stability by phase space engineering of energetic ions in tokamak plasmas.

    Science.gov (United States)

    Graves, J P; Chapman, I T; Coda, S; Lennholm, M; Albergante, M; Jucker, M

    2012-01-10

    Virtually collisionless magnetic mirror-trapped energetic ion populations often partially stabilize internally driven magnetohydrodynamic disturbances in the magnetosphere and in toroidal laboratory plasma devices such as the tokamak. This results in less frequent but dangerously enlarged plasma reorganization. Unique to the toroidal magnetic configuration are confined 'circulating' energetic particles that are not mirror trapped. Here we show that a newly discovered effect from hybrid kinetic-magnetohydrodynamic theory has been exploited in sophisticated phase space engineering techniques for controlling stability in the tokamak. These theoretical predictions have been confirmed, and the technique successfully applied in the Joint European Torus. Manipulation of auxiliary ion heating systems can create an asymmetry in the distribution of energetic circulating ions in the velocity orientated along magnetic field lines. We show the first experiments in which large sawtooth collapses have been controlled by this technique, and neoclassical tearing modes avoided, in high-performance reactor-relevant plasmas.

  19. Modelling, stability and control of voltage behaviour in power supply systems

    Energy Technology Data Exchange (ETDEWEB)

    Hill, David J [Sydney Univ., NSW (Australia). Dept. of Electrical Engineering; Hisken, Ian A [Newcastle Univ., NSW (Australia). Dept. of Electrical and Computer Engineering

    1994-12-31

    This paper gives an overview of a line of work on mid to long term voltages stability analysis and control in power systems. The results are based on use of a novel approach to dynamic load modelling using aggregate nonlinear structures. In general, the model for the transmission network and supply end dynamics is of the hybrid differential - algebraic - discrete kind. Various stability questions are precisely formulated and analysed in terms of network and load characteristics (steady-state and transient). The results are shown to be a useful framework for deriving criteria of the where, when and how much kind for various control actions such as load Thedding and tap-blocking. (author) 47 refs., 15 figs., 1 tab.

  20. Dynamics and stabilization of peak current-mode controlled buck converter with constant current load

    International Nuclear Information System (INIS)

    Leng Min-Rui; Zhou Guo-Hua; Zhang Kai-Tun; Li Zhen-Hua

    2015-01-01

    The discrete iterative map model of peak current-mode controlled buck converter with constant current load (CCL), containing the output voltage feedback and ramp compensation, is established in this paper. Based on this model the complex dynamics of this converter is investigated by analyzing bifurcation diagrams and the Lyapunov exponent spectrum. The effects of ramp compensation and output voltage feedback on the stability of the converter are investigated. Experimental results verify the simulation and theoretical analysis. The stability boundary and chaos boundary are obtained under the theoretical conditions of period-doubling bifurcation and border collision. It is found that there are four operation regions in the peak current-mode controlled buck converter with CCL due to period-doubling bifurcation and border-collision bifurcation. Research results indicate that ramp compensation can extend the stable operation range and transfer the operating mode, and output voltage feedback can eventually eliminate the coexisting fast-slow scale instability. (paper)

  1. On Stability and Control of SSTO Spaceplane in Super-and Hypersonic Ascending Phase

    OpenAIRE

    DIEKMANN, Andreas; MATSUSHIMA, Koichi; 松島, 弘一

    1991-01-01

    The natural dynamic stability of a single stage to orbit (SSTO) National Aerospace Laboratory Spaceplane Configuration is evaluated at seven reference points on a super-to hypersonic and constant dynamic pressure ascent trajectory. It is shown that dynamic instability occurs in the longitudinal and lateral motion during wide parts of the regarded trajectory. To counteract these instabilities a simple output feedback control is introduced to achieve a pole assignment of the dominant modes acco...

  2. Microprocessor supervised stability control system for the united power system of Middle Volga in fault conditions

    Energy Technology Data Exchange (ETDEWEB)

    Berdnikov, V I; Birgel, E R; Kovalev, V D; Kuznestov, A N

    1994-12-31

    The development of the 500 kV UPS of Middle Volga, the complication of its configuration and operating conditions particularly in connection with concentration of the generating power at Balakovo NPS have aggravated the problem of stability of the Middle Volga UPS when high power is transmitted along the 500 kV transient system. In this case the necessity for improving control actions` dosage accuracy has also appeared. This work discusses solution to the above mentioned issue. (author) 3 figs.

  3. Stabilization of a Wireless Networked Control System with Packet Loss and Time Delay: An ADS Approach

    Directory of Open Access Journals (Sweden)

    Jing Wang

    2012-01-01

    Full Text Available The stabilization problem of a wireless networked control system is considered in this paper. Both time delay and packet loss exist simultaneously in the wireless network. The system is modeled as an asynchronous dynamic system (ADS with unstable subsystems. A sufficient condition for the system to be stable is presented. A numerical example is given to demonstrate the effectiveness of the proposed approach.

  4. Deterministic creation and stabilization of entanglement in circuit QED by homodyne-mediated feedback control

    International Nuclear Information System (INIS)

    Liu Zhuo; Kuang Luelin; Hu Kai; Xu Luting; Wei Suhua; Guo Lingzhen; Li Xinqi

    2010-01-01

    In a solid-state circuit QED system, we demonstrate that a homodyne-current-based feedback can create and stabilize highly entangled two-qubit states in the presence of a moderate noisy environment. Particularly, we present an extended analysis for the current-based Markovian feedback, which leads to an improved feedback scheme. We show that this is essential to achieve a desirable control effect by the use of dispersive measurement.

  5. Designing a Fuzzy Logic Controller to Enhance Directional Stability of Vehicles under Difficult Maneuvers

    OpenAIRE

    Mehrdad N. Khajavi; Golamhassan Paygane; Ali Hakima

    2009-01-01

    Vehicle which are turning or maneuvering at high speeds are susceptible to sliding and subsequently deviate from desired path. In this paper the dynamics governing the Yaw/Roll behavior of a vehicle has been simulated. Two different simulations have been used one for the real vehicle, for which a fuzzy controller is designed to increase its directional stability property. The other simulation is for a hypothetical vehicle with much higher tire cornering stiffness which is ca...

  6. Stabilizing periodic orbits of chaotic systems using fuzzy control of Poincare map

    International Nuclear Information System (INIS)

    Bonakdar, Mohammad; Samadi, Mostafa; Salarieh, Hassan; Alasty, Aria

    2008-01-01

    In this paper a fuzzy control algorithm is used to stabilize the fixed points of a chaotic system. No knowledge of the dynamic equations of the system is needed in this approach and the whole system is considered as a black box. Two main approaches have been investigated: fuzzy clustering and table look up methods. As illustrative examples these methods have been applied to Bonhoeffer van der Pol oscillator and the Henon chaotic system and the convergence toward fixed points is observed

  7. Stabilizing periodic orbits of chaotic systems using fuzzy control of Poincare map

    Energy Technology Data Exchange (ETDEWEB)

    Bonakdar, Mohammad; Samadi, Mostafa [Center of Excellence in Design, Robotics and Automation (CEDRA), Department of Mechanical Engineering, Sharif University of Technology, Azadi Avenue, 1458889694 Tehran (Iran, Islamic Republic of); Salarieh, Hassan [Center of Excellence in Design, Robotics and Automation (CEDRA), Department of Mechanical Engineering, Sharif University of Technology, Azadi Avenue, 1458889694 Tehran (Iran, Islamic Republic of)], E-mail: salarieh@mech.sharif.edu; Alasty, Aria [Center of Excellence in Design, Robotics and Automation (CEDRA), Department of Mechanical Engineering, Sharif University of Technology, Azadi Avenue, 1458889694 Tehran (Iran, Islamic Republic of)

    2008-05-15

    In this paper a fuzzy control algorithm is used to stabilize the fixed points of a chaotic system. No knowledge of the dynamic equations of the system is needed in this approach and the whole system is considered as a black box. Two main approaches have been investigated: fuzzy clustering and table look up methods. As illustrative examples these methods have been applied to Bonhoeffer van der Pol oscillator and the Henon chaotic system and the convergence toward fixed points is observed.

  8. 137Cs radioactive check device for quality and stability control of dosimeters used in radiotherapy

    International Nuclear Information System (INIS)

    Alves, C.F.E.; Mondaini, C.V.; Leite, S.P.; Pires, E.J.; Magalhaes, L.A.G.; Daivd, M.G.; Almeida, C.E. de

    2015-01-01

    This paper describes the design and construction of a shielded container to store a used brachytherapy 137 Cs source. This system proposes a new option to check the periodical consistency and proper functioning of ionization chambers and electrometers used in radiotherapy. Since there is no similar equipment being produced in the Brazilian market and the chambers stability control is part of a licensing requirement this device was built to meet the needs of several radiotherapy centers without access to it. (author)

  9. Stabilization Algorithms for Automatic Control of the Trajectory Movement of Quadcopter

    Directory of Open Access Journals (Sweden)

    KeKe Gen

    2015-01-01

    Full Text Available The article considers an automatic quadcopter routing task. The quadcopter is an unmanned aerial vehicle (UAV, which has four engines. Currently, such already widely used vehicles are controlled, mainly, from the operator’s control panel. A relevant task is to develop a quadcopter control system that enables an autonomous flight. The aim of this paper is to study the possibility for solving this problem using an algorithm of the stabilization and trajectory control.A mathematical model of the quadrocopter is the fairly complicated non-linear system, which can be obtained by using the Matlab Simulink and Universal Mechanism software systems simultaneously. Comparison of the simulation results in two software packages, i.e. Matlab wherein the nonlinear system of equations is modeled and UM wherein the flight path and other parameters are calculated according to transmitted forces and moments may prove correctness of the model used.Synthesis of controllers for the orientation and stabilization subsystem and trajectory control subsystem, is performed on traditional principles, in particular using the PID controllers and method based on Lyapunov functions known in the literature as "backstepping." The most appropriate controls are selected by comparing the simulation results. Responses to the stepped impacts and to tracking the given paths have been simulated. It has been found that the flight path of a quadcopter almost coincides with designated routing, changes of coordinates for the quadcopter mass center of two controllers under comparison are almost the same, but a deviation range of the angular position for the controller backstepping is much smaller than that of for the PID controller.

  10. Mast Cell Stabilizers as Host Modulatory Drugs to Prevent and Control Periodontal Disease

    Directory of Open Access Journals (Sweden)

    Dhoom Singh Mehta

    2011-01-01

    Full Text Available Introduction: Mast cells are among the first cells to get in-volved in periodontal inflammation. Their numbers have been shown to be in-creased in cases of gingivitis and periodontal disease. The hypothesis: Since mast cell stabilizers like sodium cromogly-cate (SCG and nedocromil sodium (NS have been used in the prophylaxis of bronchial asthma without any significant adverse effects and also the fact that drugs like SCG show significant anti-inflammatory activities, it would be logical to use mast cell stabilizers as host modulating drugs for the treatment and prevention of peri-odontal disease. Evaluation of the hypothesis: Safety and efficacy of both SCG and NS are well documented. So, it will be systemically safe to use in humans. However, oral administration SCG or delivery of the drug by means local irrigation will not be very useful because SCG may not be secreted in the gingival crevicular fluid (GCF(as in the case of oral administraion or the drug may get washed out from periodontal pocket due to the constant flow of GCF(as in the case of irrigation. A local or targeted drug delivery of mast cell stabilizers can be used in patients with periodontal disease. Role of mast cells in periodontal disease has been dealt in-depth in many studies and articles. However, limited amount of research has been done on using mast cell stabilizers in the prevention and control of periodontal diseases. More studies are needed to study the efficacy and effective-ness of mast cell stabilizers as an adjunct to phase I therapy in the control of periodontal disease.

  11. Knee joint stabilization therapy in patients with osteoarthritis of the knee: a randomized, controlled trial.

    Science.gov (United States)

    Knoop, J; Dekker, J; van der Leeden, M; van der Esch, M; Thorstensson, C A; Gerritsen, M; Voorneman, R E; Peter, W F; de Rooij, M; Romviel, S; Lems, W F; Roorda, L D; Steultjens, M P M

    2013-08-01

    To investigate whether an exercise program, initially focusing on knee stabilization and subsequently on muscle strength and performance of daily activities is more effective than an exercise program focusing on muscle strength and performance of daily activities only, in reducing activity limitations in patients with knee osteoarthritis (OA) and instability of the knee joint. A single-blind, randomized, controlled trial involving 159 knee OA patients with self-reported and/or biomechanically assessed knee instability, randomly assigned to two treatment groups. Both groups received a supervised exercise program for 12 weeks, consisting of muscle strengthening exercises and training of daily activities, but only in the experimental group specific knee joint stabilization training was provided. Outcome measures included activity limitations (Western Ontario and McMaster Universities Osteoarthritis Index - WOMAC physical function, primary outcome), pain, global perceived effect and knee stability. Both treatment groups demonstrated large (∼20-40%) and clinically relevant reductions in activity limitations, pain and knee instability, which were sustained 6 months post-treatment. No differences in effectiveness between experimental and control treatment were found on WOMAC physical function (B (95% confidence interval - CI) = -0.01 (-2.58 to 2.57)) or secondary outcome measures, except for a higher global perceived effect in the experimental group (P = 0.04). Both exercise programs were highly effective in reducing activity limitations and pain and restoring knee stability in knee OA patients with instability of the knee. In knee OA patients suffering from knee instability, specific knee joint stabilization training, in addition to muscle strengthening and functional exercises, does not seem to have any additional value. Dutch Trial Register (NTR) registration number: NTR1475. Copyright © 2013 Osteoarthritis Research Society International. Published by Elsevier

  12. Stability Analysis and Trigger Control of LLC Resonant Converter for a Wide Operational Range

    Directory of Open Access Journals (Sweden)

    Zhijian Fang

    2017-09-01

    Full Text Available The gain of a LLC resonant converter can vary with the loads that can be used to improve the efficiency and power density for some special applications, where the maximum gain does not apply at the heaviest loads. However, nonlinear gain characteristics can make the converters unstable during a major disturbance. In this paper, the stability of an LLC resonant converter during a major disturbance is studied and a trigger control scheme is proposed to improve the converter’s stability by extending the converter’s operational range. Through in-depth analysis of the gain curve of the LLC resonant converter, we find that the switching frequency range is one of the key factors determining the system’s stability performance. The same result is also obtained from a mathematical point of view by utilizing the mixed potential function method. Then a trigger control method is proposed to make the LLC resonant converter stable even during a major disturbance, which can be used to extend the converter’s operational range. Finally, experimental results are given to verify the analysis and proposed control scheme.

  13. Designing of new structure PID controller of boost converter for solar photovoltaic stability

    Science.gov (United States)

    Shabrina, Hanifati Nur; Setiawan, Eko Adhi; Sabirin, Chip Rinaldi

    2017-03-01

    Nowadays, the utilization of renewable energy as the source on distributed generation system is increasing. It aims to reduce reliance and power losses from utility grid and improve power stability in near loads. One example of renewable energy technology that have been highly proven on the market is solar photovoltaic (PV). This technology converts photon from sunlight into electricity. However, the fluctuation of solar radiation that often occurs become the main problem for this system. Due to this condition, the power conversion is needed to convert the change frequently in photovoltaic panel into a stable voltage to the system. Developing control of boost converter has important role to keep ability of system stabilization. A conventional PID (Proportional, Integral, Derivative) control is mostly used to achieve this goal. In this research, a design of new structure PID controller of boost converter is offered to better optimize system stability comparing to the conventional PID. Parameters obtained from this PID structure have been successfully yield a stable boost converter output at 200 V with 10% overshoot, 1.5 seconds of settling time, and 1.5% of steady-state error.

  14. Adaptive neural networks control for camera stabilization with active suspension system

    Directory of Open Access Journals (Sweden)

    Feng Zhao

    2015-08-01

    Full Text Available The camera always suffers from image instability on the moving vehicle due to unintentional vibrations caused by road roughness. This article presents an adaptive neural network approach mixed with linear quadratic regulator control for a quarter-car active suspension system to stabilize the image captured area of the camera. An active suspension system provides extra force through the actuator which allows it to suppress vertical vibration of sprung mass. First, to deal with the road disturbance and the system uncertainties, radial basis function neural network is proposed to construct the map between the state error and the compensation component, which can correct the optimal state-feedback control law. The weights matrix of radial basis function neural network is adaptively tuned online. Then, the closed-loop stability and asymptotic convergence performance is guaranteed by Lyapunov analysis. Finally, the simulation results demonstrate that the proposed controller effectively suppresses the vibration of the camera and enhances the stabilization of the entire camera, where different excitations are considered to validate the system performance.

  15. Diagonal recurrent neural network based adaptive control of nonlinear dynamical systems using lyapunov stability criterion.

    Science.gov (United States)

    Kumar, Rajesh; Srivastava, Smriti; Gupta, J R P

    2017-03-01

    In this paper adaptive control of nonlinear dynamical systems using diagonal recurrent neural network (DRNN) is proposed. The structure of DRNN is a modification of fully connected recurrent neural network (FCRNN). Presence of self-recurrent neurons in the hidden layer of DRNN gives it an ability to capture the dynamic behaviour of the nonlinear plant under consideration (to be controlled). To ensure stability, update rules are developed using lyapunov stability criterion. These rules are then used for adjusting the various parameters of DRNN. The responses of plants obtained with DRNN are compared with those obtained when multi-layer feed forward neural network (MLFFNN) is used as a controller. Also, in example 4, FCRNN is also investigated and compared with DRNN and MLFFNN. Robustness of the proposed control scheme is also tested against parameter variations and disturbance signals. Four simulation examples including one-link robotic manipulator and inverted pendulum are considered on which the proposed controller is applied. The results so obtained show the superiority of DRNN over MLFFNN as a controller. Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.

  16. Modular Online Uninterruptible Power System Plug’n’Play Control and Stability Analysis

    DEFF Research Database (Denmark)

    Zhang, Chi; Coelho, Ernane A. A.; Guerrero, Josep M.

    2016-01-01

    In this paper, a plug`n'play control strategy proposed for modular online UPS system is presented, which allows to plug the UPS modules in or out randomly. This provides a less difficulty for the maintenance of the whole system. A two-level control scheme was proposed, including local controllers...... to achieve active and reactive power sharing and central controllers to maintain synchronization capability, which allows the online UPS modular system having faster dynamic performance according to the Standard IEC 62040-3. A detailed small signal mathematical model was developed in order to analyze...... the proposed modular online UPS system. Experimental results and data are presented to validate the stability analysis and support the proposed plug`n'play control feasibility....

  17. Model predictive controller-based multi-model control system for longitudinal stability of distributed drive electric vehicle.

    Science.gov (United States)

    Shi, Ke; Yuan, Xiaofang; Liu, Liang

    2018-01-01

    Distributed drive electric vehicle(DDEV) has been widely researched recently, its longitudinal stability is a very important research topic. Conventional wheel slip ratio control strategies are usually designed for one special operating mode and the optimal performance cannot be obtained as DDEV works under various operating modes. In this paper, a novel model predictive controller-based multi-model control system (MPC-MMCS) is proposed to solve the longitudinal stability problem of DDEV. Firstly, the operation state of DDEV is summarized as three kinds of typical operating modes. A submodel set is established to accurately represent the state value of the corresponding operating mode. Secondly, the matching degree between the state of actual DDEV and each submodel is analyzed. The matching degree is expressed as the weight coefficient and calculated by a modified recursive Bayes theorem. Thirdly, a nonlinear MPC is designed to achieve the optimal wheel slip ratio for each submodel. The optimal design of MPC is realized by parallel chaos optimization algorithm(PCOA)with computational accuracy and efficiency. Finally, the control output of MPC-MMCS is computed by the weighted output of each MPC to achieve smooth switching between operating modes. The proposed MPC-MMCS is evaluated on eight degrees of freedom(8DOF)DDEV model simulation platform and simulation results of different condition show the benefits of the proposed control system. Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.

  18. A nonlinear optimal control approach to stabilization of a macroeconomic development model

    Science.gov (United States)

    Rigatos, G.; Siano, P.; Ghosh, T.; Sarno, D.

    2017-11-01

    A nonlinear optimal (H-infinity) control approach is proposed for the problem of stabilization of the dynamics of a macroeconomic development model that is known as the Grossman-Helpman model of endogenous product cycles. The dynamics of the macroeconomic development model is divided in two parts. The first one describes economic activities in a developed country and the second part describes variation of economic activities in a country under development which tries to modify its production so as to serve the needs of the developed country. The article shows that through control of the macroeconomic model of the developed country, one can finally control the dynamics of the economy in the country under development. The control method through which this is achieved is the nonlinear H-infinity control. The macroeconomic model for the country under development undergoes approximate linearization round a temporary operating point. This is defined at each time instant by the present value of the system's state vector and the last value of the control input vector that was exerted on it. The linearization is based on Taylor series expansion and the computation of the associated Jacobian matrices. For the linearized model an H-infinity feedback controller is computed. The controller's gain is calculated by solving an algebraic Riccati equation at each iteration of the control method. The asymptotic stability of the control approach is proven through Lyapunov analysis. This assures that the state variables of the macroeconomic model of the country under development will finally converge to the designated reference values.

  19. Analysis and control of excitation, field weakening and stability in direct torque controlled electrically excited synchronous motor drives

    Energy Technology Data Exchange (ETDEWEB)

    Pyrhoenen, O

    1999-12-31

    Direct torque control (DTC) is a new control method for rotating field electrical machines. DTC controls directly the motor stator flux linkage with the stator voltage, and no stator current controllers are used. With the DTC method very good torque dynamics can be achieved. Until now, DTC has been applied to asynchronous motor drives. The purpose of this work is to analyse the applicability of DTC to electrically excited synchronous motor drives. Compared with asynchronous motor drives, electrically excited synchronous motor drives require an additional control for the rotor field current. The field current control is called excitation control in this study. The dependence of the static and dynamic performance of DTC synchronous motor drives on the excitation control has been analysed and a straightforward excitation control method has been developed and tested. In the field weakening range the stator flux linkage modulus must be reduced in order to keep the electro motive force of the synchronous motor smaller than the stator voltage and in order to maintain a sufficient voltage reserve. The dynamic performance of the DTC synchronous motor drive depends on the stator flux linkage modulus. Another important factor for the dynamic performance in the field weakening range is the excitation control. The field weakening analysis considers both dependencies. A modified excitation control method, which maximises the dynamic performance in the field weakening range, has been developed. In synchronous motor drives the load angle must be kept in a stabile working area in order to avoid loss of synchronism. The traditional vector control methods allow to adjust the load angle of the synchronous motor directly by the stator current control. In the DTC synchronous motor drive the load angle is not a directly controllable variable, but it is formed freely according to the motor`s electromagnetic state and load. The load angle can be limited indirectly by limiting the torque

  20. Analysis and control of excitation, field weakening and stability in direct torque controlled electrically excited synchronous motor drives

    Energy Technology Data Exchange (ETDEWEB)

    Pyrhoenen, O.

    1998-12-31

    Direct torque control (DTC) is a new control method for rotating field electrical machines. DTC controls directly the motor stator flux linkage with the stator voltage, and no stator current controllers are used. With the DTC method very good torque dynamics can be achieved. Until now, DTC has been applied to asynchronous motor drives. The purpose of this work is to analyse the applicability of DTC to electrically excited synchronous motor drives. Compared with asynchronous motor drives, electrically excited synchronous motor drives require an additional control for the rotor field current. The field current control is called excitation control in this study. The dependence of the static and dynamic performance of DTC synchronous motor drives on the excitation control has been analysed and a straightforward excitation control method has been developed and tested. In the field weakening range the stator flux linkage modulus must be reduced in order to keep the electro motive force of the synchronous motor smaller than the stator voltage and in order to maintain a sufficient voltage reserve. The dynamic performance of the DTC synchronous motor drive depends on the stator flux linkage modulus. Another important factor for the dynamic performance in the field weakening range is the excitation control. The field weakening analysis considers both dependencies. A modified excitation control method, which maximises the dynamic performance in the field weakening range, has been developed. In synchronous motor drives the load angle must be kept in a stabile working area in order to avoid loss of synchronism. The traditional vector control methods allow to adjust the load angle of the synchronous motor directly by the stator current control. In the DTC synchronous motor drive the load angle is not a directly controllable variable, but it is formed freely according to the motor`s electromagnetic state and load. The load angle can be limited indirectly by limiting the torque