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Sample records for controle par courants

  1. Feedback control of current drive by using hybrid wave in tokamaks; Asservissement de la generation de courant par l`onde hybride dans un plasma de tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Wijnands, T.J. [Association Euratom-CEA, Centre d`Etudes Nucleaires de Cadarache, 13 - Saint-Paul-lez-Durance (France). Dept. de Recherches sur la Fusion Controlee]|[CEA Centre d`Etudes de Cadarache, 13 - Saint-Paul-lez-Durance (France). Direction des Sciences de la Matiere

    1997-03-01

    This work is focussed on an important and recent development in present day Controlled Nuclear Fusion Research and Tokamaks. The aim is to optimise the energy confinement for a certain magnetic configuration by adapting the radial distribution of the current. Of particular interest are feedback control scenarios with stationary modifications of the current profile using current, driven by Lower Hybrid waves. A new feedback control system has been developed for Tore Supra and has made a large number of new operation scenarios possible. In one of the experiments described here, there is no energy exchange between the poloidal field system and the plasma, the current is controlled by the power of the Lower Hybrid waves while the launched wave spectrum is used to optimise the current profile shape and the energy confinement. (author) 151 refs.

  2. A method and sensor for the eddy current non destructive testing of thin tubes; Procede de controle non destructif d`un tube mince par courants de Foucault

    Energy Technology Data Exchange (ETDEWEB)

    Sartre, B.; Miller, D.; Placko, D.

    1995-06-02

    In order to control the wear (cracking or thinning) of vapor generator tubes, especially in PWR reactors, due to the friction between the tubes and dampers, an eddy current control system is proposed where the transducer is run through the tubes, and measures the tube wall thickness or tube-block clearance through impedance measurements, taking into account the variation of the sensor-to-tube distance. 8 fig.

  3. Câbles electriques - Calcul du courant admissible - Partie 1: Equations de l'intensité du courant admissible (facteur de charge 100%) et calcul des pertes - Section 2: Facteurs de pertes par courants de Foucault dans les gaines dans le cas de deux circuits disposés en nappe

    CERN Document Server

    International Electrotechnical Commission. Geneva

    1993-01-01

    Câbles electriques - Calcul du courant admissible - Partie 1: Equations de l'intensité du courant admissible (facteur de charge 100%) et calcul des pertes - Section 2: Facteurs de pertes par courants de Foucault dans les gaines dans le cas de deux circuits disposés en nappe

  4. MODÉLISATION DES FLUX DE CHALEUR GÉNÉRÉS PAR FROTTEMENT GLISSANT DANS UN CONTACT CUIVRE-ACIER TRAVERSÉ PAR UN COURANT ÉLECTRIQUE

    Directory of Open Access Journals (Sweden)

    A BOUCHOUCHA

    2001-06-01

    Full Text Available Le problème de la conduction de la chaleur dans un contact électrique glissant cuivre–acier est étudié. Le couple fonctionne dans des conditions atmosphériques et est donc refroidi par convection naturelle à travers les faces latérales. En utilisant l'équation de la chaleur, un modèle de calcul de la température interfaciale a été élaboré. A l'aide de la méthode des volumes finis, les résultats de la température en fonction de la charge normale, la vitesse de glissement et le courant électrique sont donnés. Une comparaison avec la méthode d'Archard est faite. Les résultats montrent une bonne concordance. Une discussion globale du modèle élaboré et son application dans les contacts électriques glissants a été dégagée.

  5. Surface integral formulation of Maxwell's equations for simulation of non-destructive testing by eddy currents. Preliminary study on the implementation of the fast multipole method; Formulation integrale surfacique des equations de Maxwell pour la simulation de controles non destructifs par courant de Foucault. Etude preliminaire a la mise en oeuvre de la methode multipole rapide.

    Energy Technology Data Exchange (ETDEWEB)

    Lim, T.

    2011-04-28

    numeriquement un controle non destructif par courants de Foucault (CND-CF), la reponse du capteur peut etre modelisee via une approche semi-analytique par integrales de volume. Plus rapide que la methode des elements finis, cette approche est cependant limitee a l'etude de pieces planes ou cylindriques (sans prise en compte des effets de bords) du fait de la complexite de l'expression de la dyade de Green pour des configurations plus generales. Or, il existe une forte demande industrielle pour etendre les capacites de la modelisation CF a des configurations complexes (plaques deformees, bords de piece...). Nous avons donc ete amenes a formuler differemment le probleme electromagnetique, en nous fixant comme objectif de conserver une approche semi-analytique. La formulation integrale surfacique (SIE) permet d'exprimer le probleme volumique en un probleme de transmission equivalent a l'interface (2D) entre sous-domaines homogenes. Ce probleme est ramene a la resolution d'un systeme lineaire (par la methode des moments) dont le nombre d'inconnues est reduit du fait du caractere surfacique du maillage. Des lors, ce systeme peut etre resolu par un solveur direct pour de petites configurations. Cela nous a permis de traiter plusieurs seconds membres (ie. differentes positions de capteurs) pour une seule inversion de la matrice d'impedance. Les resultats numeriques obtenus au moyen de cette formulation concernent des plaques avec la prise en compte des effets de bords tels que l'arete et le coin. Ils sont en accord avec des resultats obtenus par la methode des elements finis. Pour des configurations de grandes tailles, nous avons mene une etude preliminaire a l'adaptation d'une methode d'acceleration du produit matrice-vecteur intervenant dans un solveur iteratif (methode multipole rapide, ou FMM) afin de definir les conditions dans lesquelles le calcul FMM fonctionne correctement (precision, convergence...) dans le contexte CND

  6. Resistivity measurements using a direct current induction method (1963); Mesure de resistivite par la methode d'induction en courant continu (1963)

    Energy Technology Data Exchange (ETDEWEB)

    Delaplace, J.; Hillairet, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-01

    'il n'est pas toujours possible d'obtenir. Au contraire, la methode d'induction en courant continu que nous utilisons ne necessite aucun contact et s'applique tres bien aux echantillons de section importante. L'echantillon est place dans un champ magnetique uniforme; au moment ou l'on supprime le champ, des courants de Foucault, qui tendent a s'opposer a la disparition du champ, apparaissent dans l'echantillon. La loi de decroissance du flux magnetique dans l'echantillon permet de determiner la resistivite du materiau. Cette methode a ete appliquee a des echantillons de diametre compris entre 1 et 30 mm dans le cas de metaux bons conducteurs. Elle donne une valeur locale de la resistivite et permet d'en deceler toute variation le long d'un echantillon. Les mesures peuvent etre effectuees a toute temperature comprise entre quelques degres Kelvin et 500 deg. C. Nous avons utilise la methode d'induction pour suivre la purification du beryllium par fusion de zone: il est en effet possible d'evaluer la purete d'un materiau par des mesures de resistivite. Nous avons determine la valeur de la resistivite le long de chaque barreau de fusion de zone et localise ainsi la partie la plus pure. Des mesures a haute temperature ont ete faites sur le carbure d'uranium et sur des alliages fer-aluminium. Cette methode constitue un moyen d'investigation interessant de la resistivite des materiaux massifs. Sa precision et sa rapidite sont adaptees aussi bien aux etudes fondamentales qu'au controle de fabrication. (auteurs)

  7. Caractérisation non destructive par courants de Foucault de l'état de contraintes dans les matériaux magnétiques

    OpenAIRE

    DAHIA, Abla; Berthelot, Eric; Le Bihan, Yann; DANIEL, Laurent

    2014-01-01

    L'objectif de ce travail est d'étudier une technique de caractérisation de l'état de contraintes dans les matériaux magnétiques par la méthode du contrôle non destructif par courants de Foucault. Cette technique exploite l'effet de la contrainte sur le comportement magnétique d'un matériau magnétique. Un modèle multi-échelle a été utilisé pour décrire le comportement magnétique d'un matériau magnétique soumis à une contrainte mécanique. Un modèle éléments finis 3D a été développé pour modélis...

  8. Détermination en tridimensionnel des pertes par courant de Foucault dans la cuve d'un transformateur

    Science.gov (United States)

    Guérin, Christophe; Tanneau, Gérard; Meunier, Gérard; Sakellaris, Ioannis

    1993-03-01

    This paper deals with 3D electromagnetic modelling of a transformer so as to evaluate eddy current losses in the tank. In this purpose, several numerical methods have been used with two 3D softwares, FLUX3D (LEG) and TRIFOU (EDF). These methods use either nodal finite elements with the magnetic vector potential (FLUX3D), or the edge finite elements with the magnetic field as the state variable (TRIFOU). Classical volume elements and several special elements have been used : shell elements with the magnetic vector potential for the modelling of thin conducting regions, and surface elements for conducting regions with a small skin depth. The results given by these methods with several characteristics of the tank and magnetic core have been compared and their validity domain has been verified. The shell elements suit well when the skin depth is larger than the tank thickness, whereas in the opposite case, the surface elements for thin skin depth regions are well adapted. Nous présentons la modélisation tridimensionnelle d'un transformateur pour la détermination des pertes par courants de Foucault dans sa cuve. Pour cela, plusieurs méthodes numériques ont été utilisées à l'aide de deux codes de calcul tridimensionnels : FLUX3D (LEG) et TRIFOU (EDF). Ces méthodes utilisent soit les éléments finis nodaux avec le potentiel vecteur magnétique (FLUX3D), soit les éléments finis d'arête avec le champ magnétique comme variable d'état (TRIFOU). Outre l'utilisation des éléments volumiques, nous avons employé des éléments surfaciques de type coque en potentiel vecteur magnétique pour la modélisation de regions conductrices minces, ainsi que des éléments surfaciques pour les régions conductrices présentant une faible épaisseur de peau. Les résultats obtenus avec ces méthodes pour différentes caractéristiques du matériau de la cuve et du circuit magnétique ont permis de vérifier leur domaine de validité. Les éléments de type coque conviennent

  9. Augmentation de la densité de courant critique par dopage de YBCO texturé au BaSnO3

    Science.gov (United States)

    Lepropre, M.; Monot, I.; Delamare, M. P.; Hervieu, M.; Simon, Ch.; Provost, J.; Desgardin, G.; Raveau, B.

    1994-11-01

    _c de transport se révèlent être assez dispersées (2,5 × 10^3 à 7,1 × 10^4 A cm^{-2}). Des mesures de J_c par méthode magnétique ont été effectuées. Le résultat de ces mesures suggère la présence de microfissures dans certaines régions du matériau causant alors une chute dramatique du J_c de transport dans l'échantillon correspondant. D'autre part, les observations HREM démontrent que les défauts étendus recensés ne peuvent pas être tenus pour principaux responsables du piégeage des vortex dans ce type de matériau. L'éventuelle inhomogénéité à l'échelle nanostructurale ne semble pas influencer la densité de courant critique. Le piégeage serait donc principalement gouverné par la microstructure lamellaire et non par la présence d'inclusions de BaSnO3.

  10. Répartition du potentiel électrocinétique imposée par un dipôle de courant excentré dans une plaque conductrice rectangulaire partiellement cloisonnée

    OpenAIRE

    Troquet, J.; Lambin, Ph.

    1985-01-01

    Connaissant les distributions de potentiel électrique qui apparaissent dans une plaque conductrice rectangulaire lorsque cette dernière est alimentée par une paire source-puits parallèle à l'un ou l'autre côté, nous comparons cette situation contrôle aux conséquences d'un cloisonnement de la plaque sur un quart de sa longueur par une ou deux fentes avec pour effet habituel de réduire la transmission de courant à l'entrée de la partie cloisonnée. Corrélativement, les cloisons introduisent des ...

  11. Contrôle non destructif par courants de Foucault : expérience et modélisation pour la conception et l'optimisation de capteurs

    Science.gov (United States)

    Bonnin, O.; Cahouet, J.; Giordano, P.

    1993-03-01

    more accurate comparison, for example on the crack depth, is difficult because of the uncertainty on the physical properties of the materials. Dans le cadre d'un projet industiel de conception et d'optimisation de capteurs pour la recherche de fissures par courants de Foucault sur les plate-formes de forage, on présente les études menées pour la modélisation du système. La simulation des phénomènes implique à priori une modélisation tridimensionnelle, certes précise, mais difficile et coûteuse. On cherche à en limiter le coût en combinant une modélisation bidimensionnelle en l'absence de défaut, et une modélisation tridimensionnelle locale en présence d'une fissure. Après avoir présenté le projet et l'étude expérimentale qui en découle, on décrit les techniques de modélisation qui ont été employées.

  12. Current algebra; Algebre des courants

    Energy Technology Data Exchange (ETDEWEB)

    Jacob, M. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-07-01

    The first three chapters of these lecture notes are devoted to generalities concerning current algebra. The weak currents are defined, and their main properties given (V-A hypothesis, conserved vector current, selection rules, partially conserved axial current,...). The SU (3) x SU (3) algebra of Gell-Mann is introduced, and the general properties of the non-leptonic weak Hamiltonian are discussed. Chapters 4 to 9 are devoted to some important applications of the algebra. First one proves the Adler- Weisberger formula, in two different ways, by either the infinite momentum frame, or the near-by singularities method. In the others chapters, the latter method is the only one used. The following topics are successively dealt with: semi leptonic decays of K mesons and hyperons, Kroll- Ruderman theorem, non leptonic decays of K mesons and hyperons ( {delta}I = 1/2 rule), low energy theorems concerning processes with emission (or absorption) of a pion or a photon, super-convergence sum rules, and finally, neutrino reactions. (author) [French] La premiere partie de ce cours (trois premiers chapitres), traite des generalites concernant l'algebre de courants. Apres une definition rapide des courants faibles et un rappel de leurs proprietes (hypothese V-A, conservation du courant vecteur, regles de selection, courant axial partiellement conserve,...), l'on introduit l'algebre de Gell-Mann SU (3) x SU (3), et discute les proprietes generales de l'Hamiltonien faible non leptonique. Les chapitres IV a IX sont consacres a des applications importantes de l'algebre des courants. En premier lieu l'on demontre la formule de Adler et Weisberger, par deux methodes differentes, celle dite du repere de moment infini et celle des singularites proches. Cette derniere est seule utilisee dans la suite. Puis, l'on traite successivement les problemes suivants: desintegrations semi-leptoniques des mesons K et des hyperons, theoreme de Kroll

  13. Sélection et utilisation de l'habitat par les jeunes stades de poissons d'eau courante : le modèle Ombre commun (Thymallus thymallus, L.

    Directory of Open Access Journals (Sweden)

    SEMPESKI P.

    1995-04-01

    Full Text Available L'Ombre commun a servi de modèle biologique dans le cadre de l'étude de la dynamique des changements d'habitats caractérisant les jeunes stades de poissons d'eau courante. Des campagnes d'observations directes, depuis la berge ou en plongée, de jour comme de nuit, associées à des descriptions de l'habitat physique et des prélèvements de poissons et de faune invertébrée benthique et dérivante, ont permis une analyse fine de la sélection et de l'utilisation de l'habitat par les jeunes stades d'ombre et de leur évolution à différentes échelles spatio-temporelles. Différents groupes fonctionnels ont pu être distingués parmi les stades larvaires et juvéniles, en fonction des microhabitats sélectionnés de jour (alimentation et de nuit (repos, et des différentes stratégies de sélection des proies.

  14. Limiteurs de courant supraconducteurs

    Science.gov (United States)

    Verhaege, T.; Bekhaled, M.; Laumond, Y.; Pham, V. D.; Thomas, P.; Thérond, P. G.

    1994-04-01

    50-60 Hz electrical engineering represents a considerable potential market for superconductors. Fault current limitation will probably be the first among the possible applications, to find an industrial opening. Exploited features are very specific of the superconductors, namely the large contrast existing between an on-state with high current density and an off-state with high electrical resistivity, obtained as soon as the current reaches a specified level. It thus becomes possible to warrant the grid from any current excursion over a specified value, with considerable impact on the protection and dimensions of conductors and devices, and increased possibilities of interconnexion. The current limiter technology is rather complex ; different structures have been proposed and tested ; they are resistive or inductive, based on low-T_c or high-T_c superconductors. The most advanced candidate to date is a resistive structure, using conductors made of ultra-fine niobium-titanium filaments in a coppemickel matrix, whose essential features are described hereunder. L'électrotechnique industrielle à 50-60 Hz représente pour les supraconducteurs un marché potentiel considérable. Parmi les applications envisageables, la limitation des courants de défaut sera probablement la première à trouver un essor industriel. Les propriétés mises en jeu sont tout à fait spécifiques des supraconducteurs, à savoir le grand contraste existant entre un état passant à haute densité de courant, et un état bloquant à haute résistivité, obtenu dès que le courant dépasse un seuil donné. Il est ainsi possible de garantir le réseau contre toute excursion du courant au-delà d'une valeur spécifiée, avec un impact important sur sa protection, son dimensionnement, son degré d'interconnexion. La technologie du limiteur de courant est assez complexe ; diverses structures ont été proposées et testées, résistives ou inductives, à base de supraconducteurs BTc ou HTc. Le

  15. Déshumidification de l'air d'une serre par contact direct à courants croisés avec une solution hygroscopique organique

    Science.gov (United States)

    Chraibi, A.; Jaffrin, A.; Makhlouf, S.; Bentounes, N.

    1995-07-01

    Greenhouse air can be dehumidified by direct contact with a desicant fluid on a trickle exchanger. The water vapour extraction rate depends on the hygroscopicity of the fluid and on the exchanger efficiency. An organic fluid, the triethylene glycol (TEG) at 90% concentration, has been tested. Cross corrugated cellulosic pads, of the type used in cooling, irrigated with TEG, were placed in a wind channel to dehumidify air at various speeds and temperatures. A semi-analytical model, based on energy and mass conservation, correctly reproduces the water vapor extraction rate and the enthalpy change of both fluids. It is shown that TEG trickling through a ventilated pad of 1 m^2 area and 0.1 m thickness can be used to extract 3 to 5 kg of water vapor per hour in greenhouse climate control applications. Several pads arranged in series can be used to decrease more efficiently the absolute humidity of the air, for other applications like food drying or industrial compressed air. Une technique de déshumidification de l'air des serres consiste à le soumettre au contact d'une solution hygroscopique au sein d'un échangeur ruisselant. Le taux d'extraction de vapeur d'eau dépend à la fois du pouvoir hygroscopique de la solution et de l'efficacité de l'échangeur. Une solution hygroscopique organique, le triéthylène glycol à 90% de concentration, a été expérimentée. Un échangeur ruisselant constitué de parois de cellulose ondulées à corrugations croisées, du type “cooling pad” pour serres agricoles, a été testé pour déshumidifier de l'air dans une soufflerie expérimentale. Un modèle semi-analytique, basé sur les équations de conservation de l'énergie et de masse, permet de rendre compte des échanges et de déduire les paramètres de sortie des deux fluides en contact, à partir des caractéristiques d'entrée. Cette étude permet de chiffrer à environ 3 à 5kg/h la capacité de déshumidification d'un panneau d'un mètre carré et de 10cm d

  16. Courant Algebroids in Parabolic Geometry

    CERN Document Server

    Armstrong, Stuart

    2011-01-01

    To a smooth manifold $M$, a parabolic geometry associates a principal bundle, which has a parabolic subgroup of a semisimple Lie group as its structure group, and a Cartan connection. We show that the adjoint tractor bundle of a regular normal parabolic geometry can be endowed with the structure of a Courant algebroid. This gives a class of examples of transitive Courant algebroids that are not exact.

  17. Courant number and unsteady flow computation

    Science.gov (United States)

    Lai, Chintu; ,

    1993-01-01

    The Courant number C, the key to unsteady flow computation, is a ratio of physical wave velocity, ??, to computational signal-transmission velocity, ??, i.e., C = ??/??. In this way, it uniquely relates a physical quantity to a mathematical quantity. Because most unsteady open-channel flows are describable by a set of n characteristic equations along n characteristic paths, each represented by velocity ??i, i = 1,2,....,n, there exist as many as n components for the numerator of C. To develop a numerical model, a numerical integration must be made on each characteristic curve from an earlier point to a later point on the curve. Different numerical methods are available in unsteady flow computation due to the different paths along which the numerical integration is actually performed. For the denominator of C, the ?? defined as ?? = ?? 0 = ??x/??t has been customarily used; thus, the Courant number has the familiar form of C?? = ??/??0. This form will be referred to as ???common Courant number??? in this paper. The commonly used numerical criteria C?? for stability, neutral stability and instability, are imprecise or not universal in the sense that r0 does not always reflect the true maximum computational data-transmission speed of the scheme at hand, i.e., Ctau is no indication for the Courant constraint. In view of this , a new Courant number, called the ???natural Courant number???, Cn, that truly reflects the Courant constraint, has been defined. However, considering the numerous advantages inherent in the traditional C??, a useful and meaningful composite Courant number, denoted by C??* has been formulated from C??. It is hoped that the new aspects of the Courant number discussed herein afford the hydraulician a broader perspective, consistent criteria, and unified guidelines, with which to model various unsteady flows.

  18. Control of cleavage spindle orientation in Caenorhabditis elegans: The role of the genes par-2 and par-3

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, N.N.; Kirby, C.M.; Kemphues, K.J. [Cornell Univ., Ithaca, NY (United States)

    1995-02-01

    Polarized asymmetric divisions play important roles in the development of plants and animals. The first two embryonic cleavages of Caenorhabditis elegans provide an opportunity to study the mechanisms controlling polarized asymmetric divisions. The first cleavage is unequal, producing daughters with different sizes and fates. The daughter blastomeres divide with different orientations at the second cleavage; the anterior blastomere divides equally across the long axis of the egg, whereas the posterior blastomere divides unequally along the long axis. We report here the results of our analysis of the genes par-2 and par-3 with respect to their contribution to the polarity of these divisions. Strong loss-of-function mutations in both genes lead to an equal first cleavage and an altered second cleavage. Interestingly, the mutations exhibit striking gene-specific differences at the second cleavage. The par-2 mutations lead to transverse spindle orientations in both blastomeres, whereas par-3 mutations lead to longitudinal spindle orientations in both blastomeres. The spindle orientation defects correlate with defects in centrosome movements during both the first and the second cell cycle. Temperature shift experiments with par-2 (it5ts) indicate that the par-2(+) activity is not required after the two-cell stage. Analysis of double mutants shows that par-3 is epistatic to par-2. We propose a model wherein par-2(+) and par-3(+) act in concert during the first cell cycle to affect asymmetric modification of the cytoskeleton. This polar modification leads to different behaviors of centrosomes in the anterior and posterior and leads ultimately to blastomere-specific spindle orientations at the second cleavage. 44 refs., 5 figs., 5 tabs.

  19. Le Francais Courant: Part V, French.

    Science.gov (United States)

    Dade County Public Schools, Miami, FL.

    Instructional objectives of the Dade County Public Schools Quinmester Program in French for use with "Le Francais Courant: Part 5" focus on the development of mastery of the basic numbering system and other grammatical structures. Structures include the formation of the present tense of the irregular verbs…

  20. Eddy current probe development based on a magnetic sensor array; Developpement d'un imageur magnetique pour le controle non destructif par courants de Foucault

    Energy Technology Data Exchange (ETDEWEB)

    Vacher, F

    2007-06-15

    This research deals with in the study of the use of innovating magnetic sensors in eddy current non destructive inspection. The author reports an analysis survey of magnetic sensor performances. This survey enables the selection of magnetic sensor technologies used in non destructive inspection. He presents the state-of-the-art of eddy current probes exploiting the qualities of innovating magnetic sensors, and describes the methods enabling the use of these magnetic sensors in non destructive testing. Two main applications of innovating magnetic sensors are identified: the detection of very small defects by means of magneto-resistive sensors, and the detection of deep defects by means of giant magneto-impedances. Based on the use of modelling, optimization, signal processing tools, probes are manufactured for these both applications.

  1. Monitoring of the current profile by using cyclotronic electron waves in tokamaks; Controle du profil de courant par ondes cyclotroniques electroniques dans les tokamaks

    Energy Technology Data Exchange (ETDEWEB)

    Dumont, R

    2001-08-01

    The subject of this thesis is the study of the cyclotronic electron wave as a monitoring tool of the current profile. The first chapter is dedicated to basic notions concerning tokamak plasmas and current generation. The second chapter is centered on the use of fast electrons to generate current and on its modelling. The propagation and absorption of the cyclotronic electron wave require a specific polarization state whose characteristics must be carefully chosen according to some parameters of the discharge, the chapter 3 deals with this topic. The absorption of a wave in a plasma depends greatly on the velocity distribution of the particles that make up the plasma and this distribution is constantly modified by the energy of the wave, so this phenomenon is non-linear and its physical description is difficult. In a case of a fusion plasma, a sophisticated approximation called quasi-linear theory can be applied with some restrictions that are presented in chapter 4. Chapters 5 and 6 are dedicated to kinetics scenarios involving the low hybrid wave and the cyclotronic electron wave inside the plasma. Some experiments dedicated to the study of the cyclotronic electron wave have been performed in Tore-supra (France) and FTU (Italy) tokamaks, they are presented in the last chapter. (A.C.)

  2. The Pontryagin class for pre-Courant algebroids

    Science.gov (United States)

    Liu, Zhangju; Sheng, Yunhe; Xu, Xiaomeng

    2016-06-01

    In this paper, we show that the Jacobiator J of a pre-Courant algebroid is closed naturally. The corresponding equivalence class [J♭ ] is defined as the Pontryagin class, which is the obstruction of a pre-Courant algebroid to be deformed into a Courant algebroid. We construct a Leibniz 2-algebra and a Lie 2-algebra associated to a pre-Courant algebroid and prove that these algebraic structures are isomorphic under deformations. Finally, we introduce the twisted action of a Lie algebra on a manifold to give more examples of pre-Courant algebroids, which include the Cartan geometry.

  3. GESTION "PAR" LES COMPETENCES ET POSITIONNEMENT DU CONTROLE DE GESTION ?

    OpenAIRE

    Rouby, Evelyne; Solle, Guy

    2002-01-01

    International audience; Cet article a pour but de contribuer à une définition des contours du concept de "gestion par les compétences". De notre point de vue, ce concept relèverait des mêmes principes que la compétence organisationnelle à savoir les principes "systémique, de finalité, de spécificité, de visibilité et de reconnaissance, dynamique et cumulatif". Il consisterait à en assurer l'interaction dynamique entre les niveaux stratégiques et opérationnels et selon des cycles temporels dif...

  4. Lumbar lordosis and pars interarticularis fractures: a case-control study.

    Science.gov (United States)

    Bugg, William G; Lewis, Mark; Juette, Arne; Cahir, John G; Toms, Andoni P

    2012-07-01

    The aim of this study is to examine the relationship between lumbar lordosis and pars interarticularis fractures. In this retrospective case-control study we compare the angle of lumbar lordosis and the angle of the S1 vertebral endplate (as a measure of pelvic tilt) in patients with bilateral L5 pars interarticularis fractures with age- and sex-matched control cases with normal MRI examinations of the lumbar spine. Twenty-nine cases of bilateral L5 pars interarticularis fractures with matched control-cases were identified on MRI (16 male, 13 female, age 9-63 years). The angle of lordosis was measured between the inferior L4 and superior S1 vertebral endplates on a standing lateral lumbar spine radiograph for both groups. The mean angle of lordosis about the L5 vertebra was 36.9° (SD = 6.5°) in the pars interarticularis fracture group, and 30.1° (SD = 6.4°) in the control group. The difference between the two groups was significant (mean difference 6.8°, Student's t test: P lordosis, between L4 and S1, and pars fractures at L5. Steep angulation of the first sacral vertebral segment maybe the predisposing biomechanical factor that leads to pincer-like impingement of the pars interarticularis and then spondylolysis.

  5. LA COMPTABILITÉ DES ACTIFS OU DES IMMOBILISATIONS NON COURANTS ET DISPONIBLES POUR VENTE

    Directory of Open Access Journals (Sweden)

    Lucian-Dorel ILINCUŢĂ

    2010-06-01

    Full Text Available IFRS 5 Actifs non courants destinés à être vendus et activités abandonnées prévoie qu’une entité cataloguera un actif immobilisé (ou groupe d’actifs destinés à la cession comme destiné à être vendu, si sa valeur comptable est récupérée premièrement par une opération de vente et non pas par son utilisation continue. Les règlements comptables de Roumanie prévoient le transfert des actifs immobilisés destinés à être vendus aux stocks.

  6. Lumbar lordosis and pars interarticularis fractures: a case-control study

    Energy Technology Data Exchange (ETDEWEB)

    Bugg, William G.; Lewis, Mark; Juette, Arne; Cahir, John G.; Toms, Andoni P. [Cotman Centre, Norwich Radiology Academy, Norwich, Norfolk (United Kingdom)

    2012-07-15

    The aim of this study is to examine the relationship between lumbar lordosis and pars interarticularis fractures. In this retrospective case-control study we compare the angle of lumbar lordosis and the angle of the S1 vertebral endplate (as a measure of pelvic tilt) in patients with bilateral L5 pars interarticularis fractures with age- and sex-matched control cases with normal MRI examinations of the lumbar spine. Twenty-nine cases of bilateral L5 pars interarticularis fractures with matched control-cases were identified on MRI (16 male, 13 female, age 9-63 years). The angle of lordosis was measured between the inferior L4 and superior S1 vertebral endplates on a standing lateral lumbar spine radiograph for both groups. The mean angle of lordosis about the L5 vertebra was 36.9 (SD = 6.5 ) in the pars interarticularis fracture group, and 30.1 (SD = 6.4 ) in the control group. The difference between the two groups was significant (mean difference 6.8 , Student's t test: P < 0.001). The mean angle of sacral tilt measured was 122.2 (SD = 10.16 ) for controls and 136.4 (SD = 10.86 ) for patients with pars defects. The difference in the means of 14.2 was statistically significantly different (P < 0.0001). Sacral tilt represented by a steeply angled superior endplate of S1 is associated with a significantly increased angle of lordosis, between L4 and S1, and pars fractures at L5. Steep angulation of the first sacral vertebral segment maybe the predisposing biomechanical factor that leads to pincer-like impingement of the pars interarticularis and then spondylolysis. (orig.)

  7. EXPLICIT ERROR ESTIMATES FOR COURANT,CROUZEIX-RAVIART AND RAVIART-THOMAS FINITE ELEMENT METHODS

    Institute of Scientific and Technical Information of China (English)

    Carsten Carstensen; Joscha Gedicke; Donsub Rim

    2012-01-01

    The elementary analysis of this paper presents explicit expressions of the constants in the a priori error estimates for the lowest-order Courant,Crouzeix-Raviart nonconforming and Raviart-Thomas mixed finite element methods in the Poisson model problem.The three constants and their dependences on some maximal angle in the triangulation are indeed all comparable and allow accurate a priori error control.

  8. Etude par spectroscopie de Coulomb de points quantiques lateraux individuels et couples

    Science.gov (United States)

    Pioro-Ladriere, Michel

    Des points quantiques contenant un nombre discret et variable d'electrons sont formes dans un gaz bi-dimensionnel d'electrons a l'aide de grilles metalliques. Le transport electrique, le blocage de spin et la detection de charge sont employes comme outils spectroscopiques permettant de sonder les proprietes de ces nanostructures. Ces techniques permettent aussi de controler exactement le nombres d'electrons confines dans des points quantiques individuels et couples en utilisant un patron de grille judicieux. Une technique de refroidissement en tension est developpee afin de minimiser les effets parasites du bruit telegraphique. Ce type de bruit de charge deteriore la stabilite des nanostructures laterales par l'activation d'un minuscule courant de fuite entre les grilles et le gaz bi-dimensionnel. Un modele expliquant le role du refroidissement en tension sur le courant de fuite est presente. L'activation du courant de fuite est confirmee par detection de charge. Les effets des interactions entre les electrons pieges dans un point quantique sont ensuite etudies dans un regime ou il est possible de comparer les resulats experimentaux avec ceux obtenus par diagonalisation exacte. L'etude demontre que la phase associee au facteur de remplissage nu = 2 est instable au-dessus d'un nombre critique d'electrons. Cette instabilite est confirmee experimentalement par blocage de spin. On demontre aussi l'existence d'etats correles dans le regime des renversements de spin, associe au passage de la phase nu = 2 a nu = 1. Les etats correles sont identifies par spectroscopie en transport non lineaire. Cette caracterisation du diagramme de phase de points individuels permet de coupler deux points quantiques configures a nu = 2. Pour ce regime, la nanostructure se comporte comme un systeme a deux niveaux pouvant contenir entre un et quatre electrons de valence et ce, meme si le nombre total d'electrons est plus eleve. Les degres de liberte de charge et de spin des deux points

  9. Circuito de control de par y velocidad de un servomotor

    OpenAIRE

    Gómez Llopis, Alberto Domingo

    2015-01-01

    Este Proyecto de Final de Carrera es un circuito de regulación de velocidad y sentido de giro de un servomotor de corriente continua, que tiene como voltaje de entrada 200 voltios de corriente continua proporcionada por una batería. Esta batería proporciona la energía necesaria para la alimentación del sistema de control electrónico y del servomotor. El servomotor es un MT-4525-BTYCN fabricado por Baldor, el cual trabaja a un tensión de 150 voltios, una corriente nominal de 6.16 amperio...

  10. Circuito de control de par y velocidad de un servomotor

    OpenAIRE

    Gómez Llopis, Alberto Domingo

    2014-01-01

    Este Proyecto de Final de Carrera es un circuito de regulación de velocidad y sentido de giro de un servomotor de corriente continua, que tiene como voltaje de entrada 200 voltios de corriente continua proporcionada por una batería. Esta batería proporciona la energía necesaria para la alimentación del sistema de control electrónico y del servomotor. El servomotor es un MT-4525-BTYCN fabricado por Baldor, el cual trabaja a un tensión de 150 voltios, una corriente nominal de 6.16 amperio...

  11. Controle de la fabrication des composites par injection sur renforts

    Science.gov (United States)

    Lebel, Francois

    Liquid Composite Molding (LCM) is an increasingly used class of processes to manufacture high performance composites. A multiscale study is presented in this thesis in order to better understand the fundamental physics of impregnation and air entrapment phenomena in dual scale fibrous reinforcements and thus propose practical solutions for process control engineers. First of all, an experimental setup is developed to study the saturation of fibrous reinforcements, at the macroscopic scale, during the Resin Transfer Molding (RTM). This setup is used to determine some key parameters of the part filling step and industrial post-filling strategies (mold bleeding and consolidation) that control the impregnation quality of fibrous reinforcements. These key parameters are identified using three series of experiments. These parameters are the flow front velocity, the inlet mold pressure and the bleeding flow rate. The analyses in these three series of experiments are based on an ASTM standard procedure for void content determination in the composite parts by carbonization (also called loss on ignition (LOI)). These three series of experiments have related a posteriori the key parameters of LCM processes to phenomena of void formation, migration and dissolution in composite parts made of E-glass non crimp fabric (NCF) and vinyl ester resin. The second part of this thesis aims to investigate, at the mesoscopic and microscopic scale, the impregnation mechanisms of fibrous reinforcements during LCM processes. This analysis focuses more specifically on wicking phenomenon in fiber tows and in fibrous laminates, namely here stacks of non crimp fabric plies. This experimental study is carried out to better understand the physics which explain that the impregnation velocity is one of the key parameters that control the quality of composite parts manufactured by LCM processes. This analysis also aims to identify the structural features of fiber tows and fibrous reinforcements that

  12. Identification and real time control of current profile in Tore-supra: algorithms and simulation; Identification et controle en temps reel du profil de courant dans Tore Supra: algorithmes et simulations

    Energy Technology Data Exchange (ETDEWEB)

    Houy, P

    1999-10-15

    The aim of this work is to propose a real-time control of the current profile in order to achieve reproducible operating modes with improved energetic confinement in tokamaks. The determination of the profile is based on measurements given by interferometry and polarimetry diagnostics. Different ways to evaluate and improve the accuracy of these measurements are exposed. The position and the shape of a plasma are controlled by the poloidal system that forces them to cope with standard values. Gas or neutral ions or ice pellet or extra power injection are technical means used to control other plasma parameters. These controls are performed by servo-controlled loops. The poloidal system of Tore-supra is presented. The main obstacle to a reliable determination of the current profile is the fact that slightly different Faraday angles lead to very different profiles. The direct identification method that is exposed in this work, gives the profile that minimizes the square of the margin between measured and computed values. The different algorithms proposed to control current profiles on Tore-supra have been validated by using a plasma simulation. The code Cronos that solves the resistive diffusion equation of current has been used. (A.C.)

  13. Acquis en électrocinétique à courant continu

    OpenAIRE

    Calmettes, B.

    2013-01-01

    Nous présentons une étude comparée de deux groupes d'élèves de classes de première, section Electrotechnique, de Lycée Technique. Leurs passés scolaires diffèrent : enseignements classique en seconde pour un groupe et professionnalisé en L. E. P. (Lycée d'Enseignement Professionnel) pour l'autre. Nous avons abordé trois thèmes relatifs à l'électrocinétique à courant continu : les conceptions et leur instabilité, la pratique courante de l'algèbre élémentaire en Physique Appliquée, l'utilisatio...

  14. Courant-Snyder invariant density screening method for emittance analysis

    Institute of Scientific and Technical Information of China (English)

    SUN Ji-Lei; TANG Jing-Yu; JING Han-Tao

    2011-01-01

    Emittance is an important characteristic of describing charged particle beams.In hadron accelerators,we often meet irregular beam distributions that are not appropriately described by a single rms emittance or 95% emittance or total emittance.In this paper,it is pointed out that in many cases a beam halo should be described with very different Courant-Snyder parameters from the ones used for the beam core.A new method - the Courant-Snyder invariant density screening method - is introduced for analyzing emittance data clearly and accurately.The method treats the emittance data from both measurements and numerical simulations.The method uses the statistical distribution of the beam around each particle in phase space to mark its local density parameter,and then uses the density distribution to calculate the beam parameters such as the Courant-Snyder parameters and emittance for different beam boundary definitions.The method has been used in the calculations for.beams from different sources,and shows its advantages over other methods.An application code based on the method including the graphic interface has also been designed.

  15. Development and validation of a simulation tool dedicated to eddy current non destructive testing of tubes; Developpement d'un modele electromagnetique 3D pour la simulation du controle par Courants de Foucault de tubes en fabrication

    Energy Technology Data Exchange (ETDEWEB)

    Reboud, Ch

    2006-09-15

    Eddy current testing (ECT) technique is widely used in industrial fields such as iron and steel industry. Dedicated simulation tools provide a great assistance for the optimisation of ECT processes. CEA and the Vallourec Research Center have collaborated in order to develop a simulation tool of ECT of tubes. The volume integral method has been chosen for the resolution of Maxwell equations in a stratified medium, in order to get accurate results with a computation time short enough to carry out optimisation or inversion procedures. A fast model has been developed for the simulation of ECT of non magnetic tubes using specific external probes. New flaw geometries have been modelled: holes and notches with flat bottom. Validations of the developments, which have been integrated to the CIVA platform, have been carried out using experimental data recorded in laboratory conditions and in. industrial conditions, successively. The integral equations derived are solved using the Galerkin variant of the method of moments with pulse functions as projection functions. In order to overcome some memory limitations, other projection functions have been considered. A new discretization scheme based on non-uniform B-Splines of degree 1 or 2 has been implemented, which constitutes an original contribution to the existing literature. The decrease of the mesh size needed to get a given accuracy on the result may lead to the simulation of more complex ECT configurations. (author)

  16. Development of a 3-D model for eddy current testing: application for fastened structures in aeronautics; Developpement d'un modele pour le controle non destructif par courants de Foucault de structures rivetees en aeronautique

    Energy Technology Data Exchange (ETDEWEB)

    Paillard, S

    2007-12-15

    One of the Eddy Current Testing issues in aeronautics is the inspection of fastened structures to detect flaws nearby rivets which can grow because of mechanical stress. EADS and the CEA LIST have started a collaborative work with the support of the Ile-de-France Region to develop a simulation tool of EC fastened structures testing, integrated to the CIVA platform, aimed at conceiving testing methods, optimizing and qualifying it. The volume integral method using the Green dyadics formalism has been chosen in order to get a fast resolution of Maxwell equations. A first milestone was to build a simulation model of multilayer structures testing, thanks to the use of the multilayer Green dyads. Because of the rivet volume, 60 times bigger than the one of a typical flaw, a large number of discretization cells are needed. Therefore an iterative method has been developed in order to numerically solve large calculation zones. Finally, the flaw response simulation mostly has to cope with a scale issue between the size of the rivet and the one of the flaw, the latter being much smaller in a direction than the former. The whole model has been experimentally validated and compared to other simulation models at the important development steps: multilayer configuration, iteration resolution, and flaw signature. (author)

  17. Topological Membranes, Current Algebras and H-flux - R-flux Duality based on Courant Algebroids

    CERN Document Server

    Bessho, Taiki; Ikeda, Noriaki; Watamura, Satoshi

    2015-01-01

    We construct a topological sigma model and a current algebra based on a Courant algebroid structure on a Poisson manifold. In order to construct models, we reformulate the Poisson Courant algebroid by supergeometric construction on a QP-manifold. A new duality of Courant algebroids which transforms H-flux and R-flux is proposed, where the transformation is interpreted as a canonical transformation of a graded symplectic manifold.

  18. Contrôle d'une machine asynchrone par estimation robuste de la vitesse

    Science.gov (United States)

    Roboam, X.; Hapiot, J. C.; de Fornel, B.; Andrieux, C.

    1992-03-01

    This paper describes the study and the implementation of a variable speed drive for induction motor with no mechanical sensor. Power is supplied by a current-controlled voltage-source inverter. The lack of information (only two line-current sensors) requires an accurate and reliable reconstitution of the main quantities not sensed (flux, speed, torque). The results obtained show the excellent quality of the estimated speed, whatever the operating condition and despite the perturbations applied to the system. This study finally leads to the construction of a prototype. Cet article décrit l'étude et la mise en oeuvre d'un variateur de vitesse pour machine asynchrone sans capteur mécanique et alimentée par un onduleur de tension cotrôlé en courant. Le manque d'information (2 capteurs de courants de ligne uniquement) nécessite la reconstitution fiable et précise des grandeurs fondamentales non mesurées (flux, vitesse, couple). Les résultats obtenus montrent notamment l'excellente qualité de l'estimation de vitesse, quel que soit le point de fonctionnement et en dépit des perturbations auxquelles le système est soumis. Cette étude donne lieu à la réalisation d'un variateur prototype.

  19. Slmb antagonises the aPKC/Par-6 complex to control oocyte and epithelial polarity.

    Science.gov (United States)

    Morais-de-Sá, Eurico; Mukherjee, Avik; Lowe, Nick; St Johnston, Daniel

    2014-08-01

    The Drosophila anterior-posterior axis is specified when the posterior follicle cells signal to polarise the oocyte, leading to the anterior/lateral localisation of the Par-6/aPKC complex and the posterior recruitment of Par-1, which induces a microtubule reorganisation that localises bicoid and oskar mRNAs. Here we show that oocyte polarity requires Slmb, the substrate specificity subunit of the SCF E3 ubiquitin ligase that targets proteins for degradation. The Par-6/aPKC complex is ectopically localised to the posterior of slmb mutant oocytes, and Par-1 and oskar mRNA are mislocalised. Slmb appears to play a related role in epithelial follicle cells, as large slmb mutant clones disrupt epithelial organisation, whereas small clones show an expansion of the apical domain, with increased accumulation of apical polarity factors at the apical cortex. The levels of aPKC and Par-6 are significantly increased in slmb mutants, whereas Baz is slightly reduced. Thus, Slmb may induce the polarisation of the anterior-posterior axis of the oocyte by targeting the Par-6/aPKC complex for degradation at the oocyte posterior. Consistent with this, overexpression of the aPKC antagonist Lgl strongly rescues the polarity defects of slmb mutant germline clones. The role of Slmb in oocyte polarity raises an intriguing parallel with C. elegans axis formation, in which PAR-2 excludes the anterior PAR complex from the posterior cortex to induce polarity, but its function can be substituted by overexpressing Lgl.

  20. Filtrage actif des harmoniques en courant et en tension des reseaux électriques : modélisation, simulation numérique et expérimentation

    Science.gov (United States)

    Lott, C.; Lapierre, O.; Pouliquen, H.; Saadate, S.

    1997-12-01

    Static converters generate harmonic currents and consume reactive power. These new phenomena on the mains generate some perturbations going from malfunction to destruction of the connected sensible equipments. In this paper disturbances generated by static converters are studied. Some solutions based on passive and active filtering are presented. Some passive filter design elements are given. Active filter is then studied. Both structures, current and voltage, are studied and voltage source structure is chosen. Different active filter control principles are presented. The active and reactive instantaneous power theory give good results for harmonic identification. Some numerical simulation are realized. A 100kVA prototype is realized and tested on an industrial site. The GTO prototype shows good results for harmonic filtering. The voltage active filtering is also presented. The active filter eliminates voltage harmonics on 400 V busbar generated by a MVA SCR bridge connected on 15kV busbar. Les convertisseurs statiques absorbent des courants non sinusoïdidaux et consomment généralement de la puissance réactive. Ces deux phénomènes nouveaux sur le réseau ont engendré un certain nombre de perturbations allant du dysfonctionnement d'un équipement jusqu'à la destruction d'une partie des équipements connectés. Dans cet article, les problèmes liés aux perturbations générées par les convertisseurs statiques ont été étudiés et des solutions visant à les éliminer ont été présentées. Elles portent sur les méthodes de filtrage passif et actif. Quelques éléments de définition et de dimensionnement de filtres passifs sont donnés. Le filtrage actif est ensuite traité. Les deux structures, courant et tension, sont étudiées et la structure tension a été retenue pour la suite de l'article. Différents principes de contrôle du filtre actif à structure tension ont été présentés. La méthode d'identification des harmoniques par le principe du

  1. Generalized Courant-Snyder theory for coupled transverse dynamics of charged particles in electromagnetic focusing lattices

    Directory of Open Access Journals (Sweden)

    Hong Qin

    2009-06-01

    Full Text Available The Courant-Snyder theory gives a complete description of the uncoupled transverse dynamics of charged particles in electromagnetic focusing lattices. In this paper, the Courant-Snyder theory is generalized to the case of coupled transverse dynamics with two degrees of freedom. The generalized theory has the same structure as the original Courant-Snyder theory for one degree of freedom. The four basic components of the original Courant-Snyder theory, i.e., the envelope equation, phase advance, transfer matrix, and the Courant-Snyder invariant, all have their counterparts, with remarkably similar expressions, in the generalized theory presented here. In the generalized theory, the envelope function is generalized into an envelope matrix, and the envelope equation becomes a matrix envelope equation with matrix operations that are noncommutative. The generalized theory gives a new parametrization of the 4D symplectic transfer matrix that has the same structure as the parametrization of the 2D symplectic transfer matrix in the original Courant-Snyder theory. All of the parameters used in the generalized Courant-Snyder theory correspond to physical quantities of importance, and this parametrization can provide a valuable framework for accelerator design and particle simulation studies. A time-dependent canonical transformation is used to develop the generalized Courant-Snyder theory. Applications of the new theory to strongly and weakly coupled dynamics are given. It is shown that the stability of coupled dynamics can be determined by the generalized phase advance developed. Two stability criteria are given, which recover the known results about sum and difference resonances in the weakly coupled limit.

  2. Thermal control of electronic equipment by heat pipes; Controle thermique de composants electroniques par caloducs

    Energy Technology Data Exchange (ETDEWEB)

    Groll, M.; Schneider, M. [Stuttgart Univ. (Germany). Inst. fuer Kernenergetik und Energiesysteme; Sartre, V.; Chaker Zaghdoudi, M.; Lallemand, M. [Institut National des Sciences Appliquees (INSA), 69 - Villeurbanne (France). Centre de Thermique de Lyon, Upresa CNRS

    1998-05-01

    In the frame of the BRITE-EURAM european programme (KHIEPCOOL project), a literature survey on the main beat pipe and micro heat pipe technologies developed for thermal control of electronic equipment has been carried out. The conventional heat pipes are cylindrical, flat or bellow tubes, using wicks or axial grooves as capillary structures. In the field of micro heat pipes, the component interconnection substrate. The best performances were achieved with Plesch`s axially grooved flat miniature heat pipe, which is able to transfer a heat flux of about 60 W.cm{sup -2}. Theoretical models have shown that the performance of micro heat pipe arrays increase with increasing tube diameter, decreasing tube length and increasing heat pipe density. The heat pipe technologies are classified and compared according to their geometry and location in the system. A list of about 150 references, classified according to their subjects, is presented. (authors) 160 refs.

  3. Infection prevention and control in dental surgeries in the Pará state prison system in Brazil.

    Science.gov (United States)

    Lima, Clelia Maria A; Smith, Andrew J; Fonseca Silva, Almenara S; Flório, Flávia M; Zanin, Luciane

    2016-11-01

    Prison populations have higher levels of bloodborne viruses with consequently higher risks of cross-infection. This study assessed infection prevention and occupational hazards in prison dental surgeries in the Brazilian state of Pará. Investigations were undertaken by a single examiner in 11 prison clinics. Manual washing of instruments with no detergents and dry heat sterilization were the main instrument decontamination methods used. Most surgeries had insufficient instruments for daily clinical work requirements. Half of dentists interviewed worked single-handed with no documented policies or health and safety procedures. Copyright © 2016 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

  4. Process for increasing the signal to noise ratio in inspection of metallic tubes by eddy currents; Procede permettant de reduire le bruit de fond au cours du controle de tubes metalliques par courant de Foucault et tubes realises par ce procede

    Energy Technology Data Exchange (ETDEWEB)

    Buret, J.L.; Vuillaume, D.

    1995-02-10

    The process for increasing the signal to noise ratio in inspection of metallic tubes which have been cold pigger rolled consists to subject the tube to one or more drawing passes to reduce its external diameter.

  5. Sociologie de l’alimentation : les cinq portes de l’entrée par les familles 

    OpenAIRE

    Nicolas Belorgey

    2011-01-01

    La sociologie de l’alimentation s’est peu à peu autonomisée par rapport à la sociologie de la famille. Les deux corpus ne tirent pas aujourd'hui tout le parti de ce qu’ils ont en commun. Ce texte propose un inventaire des courants de recherche, sociologiques ou non, qui s’intéressent à la famille et qui fournissent des éléments théoriques mobilisables ou objectivables pour la sociologie de l’alimentation. On peut identifier ainsi cinq grands courants : l’approche statistique fondée sur les ca...

  6. Analysis of continuously rotating quadrupole focusing channels using generalized Courant-Snyder theory

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Moses; Qin, Hong; Gilson, Erik; Davidson, Ronald C.

    2013-01-01

    By extending the recently developed generalized Courant-Snyder theory for coupled transverse beam dynamics, we have constructed the Gaussian beam distribution and its projections with arbitrary mode emittance ratios. The new formulation has been applied to a continuously-rotating quadrupole focusing channel because the basic properties of this channel are known theoretically and could also be investigated experimentally in a compact setup such as the linear Paul trap configuration. The new formulation retains a remarkably similar mathematical structure to the original Courant-Snyder theory, and thus provides a powerful theoretical tool to investigate coupled transverse beam dynamics in general and more complex linear focusing channels.

  7. Broca Pars Triangularis Constitutes a “Hub” of the Language-Control Network during Simultaneous Language Translation

    Directory of Open Access Journals (Sweden)

    Stefan Elmer

    2016-09-01

    Full Text Available Until now, several branches of research have fundamentally contributed to a better understanding of the ramifications of bilingualism, multilingualism, and language expertise on psycholinguistic-, cognitive-, and neural implications. In this context, it is noteworthy to mention that from a cognitive perspective, there is a strong convergence of data pointing to an influence of multilingual speech competence on a variety of cognitive functions, including attention, short-term- and working memory, set shifting, switching, and inhibition. In addition, complementary neuroimaging findings have highlighted a specific set of cortical and subcortical brain regions which fundamentally contribute to administrate cognitive control in the multilingual brain, namely Broca’s area, the middle-anterior cingulate cortex, the inferior parietal lobe, and the basal ganglia. However, a disadvantage of focusing on group analyses is that this procedure only enables an approximation of the neural networks shared within a population while at the same time smoothing inter-individual differences. In order to address both commonalities (i.e., within group analyses and inter-individual variability (i.e., single-subject analyses in language control mechanisms, here I measured five professional simultaneous interpreters while the participants overtly translated or repeated sentences with a simple subject-verb-object structure. Results demonstrated that pars triangularis was commonly activated across participants during backward translation (i.e., from L2 to L1, whereas the other brain regions of the control network showed a strong inter-individual variability during both backward and forward (i.e., from L1 to L2 translation. Thus, I propose that pars triangularis plays a crucial role within the language-control network and behaves as a fundamental processing entity supporting simultaneous language translation.

  8. Heterotic Reduction of Courant Algebroid Connections and Einstein-Hilbert Actions

    CERN Document Server

    Jurco, Branislav

    2015-01-01

    We discuss Levi-Civita connections on Courant algebroids. We define an appropriate generalization of the curvature tensor and compute the corresponding scalar curvatures in the exact and heterotic case, leading to generalized (bosonic) Einstein-Hilbert type of actions known from supergravity. In particular, we carefully analyze the process of the reduction for the generalized metric, connection, curvature tensor and the scalar curvature.

  9. Heterotic reduction of Courant algebroid connections and Einstein–Hilbert actions

    Energy Technology Data Exchange (ETDEWEB)

    Jurčo, Branislav, E-mail: jurco@karlin.mff.cuni.cz [Mathematical Institute, Faculty of Mathematics and Physics, Charles University, Prague 186 75 (Czech Republic); Vysoký, Jan, E-mail: vysoky@math.cas.cz [Institute of Mathematics of the Czech Academy of Sciences, Žitná 25, Prague 115 67 (Czech Republic); Mathematical Sciences Institute, Australian National University, Acton ACT 2601 (Australia)

    2016-08-15

    We discuss Levi-Civita connections on Courant algebroids. We define an appropriate generalization of the curvature tensor and compute the corresponding scalar curvatures in the exact and heterotic case, leading to generalized (bosonic) Einstein–Hilbert type of actions known from supergravity. In particular, we carefully analyze the process of the reduction for the generalized metric, connection, curvature tensor and the scalar curvature.

  10. A Dual Mechanism Controls Nuclear Localization in the Atypical Basic-Helix-Loop-Helix Protein PAR1 of Arabidopsis thaliana

    Institute of Scientific and Technical Information of China (English)

    Anahit Galstyan; Jordi Bou-Torrent; Irma Roig-Villanova; Jaime F. Martínez-García

    2012-01-01

    PAR1 is an atypical basic-helix-loop-helix (bHLH) protein that negatively regulates the shade avoidance syndrome in Arabidopsis thaliana acting as a transcriptional cofactor.Consistently with this function,PAR1 has to be in the nucleus to display biological activity.Previous structure-function analyses revealed that the N-terminal region of PAR1 drives the protein to the nucleus.However,truncated forms of PAR1 lacking this region still display biological activity,implying that PAR1 has additional mechanisms to localize into the nucleus.In this work,we compared the primary structure of PAR1 and various related and unrelated plant bHLH proteins,which led us to suggest that PAR1 contains a non-canonical nuclear localization signal (NLS) in the N-terminal region.By overexpressing truncated and mutated derivatives of PAR1,we have also investigated the importance of other regions of PAR1,such as the acidic and the extended HLH dimerization domains,for its nuclear localization.We found that,in the absence of the N-terminal region,a functional HLH domain is required for nuclear localization.Our results suggest the existence of a dual mechanism for PAR1 nuclear localization:(1) one mediated by the N-terminal non-consensus NLS and (2) a second one that involves interaction with other proteins via the dimerization domain.

  11. Controle sismique d'un batiment en acier de 1 etage par amortisseurs elastomeres et contreventements en Chevron

    Science.gov (United States)

    Girard, Olivier

    Actuellement, le principe de dimensionnement a la capacite est fortement utilise dans le domaine du genie parasismique. De maniere simplifiee, cette methode de dimensionnement consiste a dissiper l'energie injectee a une structure lors d'une secousse sismique par la deformation inelastique d'un element structural sacrificiel. Cette methode de dimensionne-ment permet d'obtenir des structures economiques, car cette dissipation d'energie permet de reduire substantiellement les efforts qui se retrouvent a l'interieur de la structure. Or, la consequence de ce dimensionnement est la presence de degats importants a la structure qui suivent a la secousse sismique. Ces degats peuvent engendrer des couts superieurs aux couts d'erection de la structure. Bien entendu, sachant que les secousses sismiques d'importances sont des phenomenes rares, l'ingenieur est pret a accepter ce risque afin de diminuer les couts initiaux de construction. Malgre que cette methode ait permis d'obtenir des constructions economiques et securitaires, il serait interessant de developper un systeme qui permettrait d'obtenir des performances de controle des efforts sismiques comparables a un systeme dimensionne selon un principe de dimensionnement a la capacite sans les consequences negatives de ces systemes. En utilisant les principes d'isolation a la base, il a ete possible de developper un systeme de reprise des forces sismiques (SRFS). qui permet d'obtenir un controle des efforts sismiques concurrentiels tout en gardant une structure completement elastique. Ce systeme consiste u inserer un materiel elastomere entre l'assemblage de la poutre et des contreventements a l'interieur d'un cadre contrevente conventionnel. Cette insertion permet de diminuer substantiellement la rigidite laterale du batiment, ce qui a pour consequence d'augmenter la valeur de la periode fondamentale du batiment dans lequel ces cadres sont inseres. Ce phenomene est appele le saut de periode. Ce saut de periode permet de

  12. La mise en oeuvre des codes d'éthique par le control interne

    NARCIS (Netherlands)

    van der Elst, C.F.

    2014-01-01

    The real value of the internal control system lies in the animation of the ethical values in the organization. The integration of ethical risks in the internal control process enables the moving notions of ethical behaviour and accommodate the training and inducement programs of all employees accord

  13. Monitoreo de parásitos en efluentes domiciliarios Control of parasites in domestic sewage

    Directory of Open Access Journals (Sweden)

    Liliana Semenas

    1999-08-01

    Full Text Available OBJETIVO: Evaluar la presencia de parásitos en efluentes semisólidos y líquidos en distintas localidades de la Patagonia argentina considerando que ésta es una de las restricciones para su uso. MÉTODOS: Las muestras tomadas en 4 Plantas de Tratamiento de Efluentes Domiciliarios se analizaron siguiendo las normativas de Environmental Protection Agency, Organización Mundial de la Salud, Standard Methods for Examination of Water and Wastewater y de algunas clasificaciones. RESULTADOS: Solamente 2 de las 6 muestras de semisólidos analizadas tenían huevos de Ascaris lumbricoides no viables. De las 10 muestras líquidas, solamente 2 no tenían huevos mientras las restantes tenían patógenos de las categorías I (Giardia sp., Hymenolepis diminuta y Enterobius vermicularis y III (Ascaris lumbricoides, Ancylostoma duodenale y Trichuris trichiura. CONCLUSIONES: Todas las muestras de semisólidos analizadas fueron aptas para su uso como fertilizantes porque no se registró en ninguna de ellas la presencia de huevos viables de Ascaris lumbricoides y solamente 6 de las muestras líquidas fueron aptas para riego por carecer de huevos o por ser su concentración igual o inferior a 1 huevo por litro.OBJETIVO: Avaliar a presença de parasitas em efluentes semi-sólidos e líquidos, em diferentes localidades da Patagonia argentina, por ser fato uma das restrições para seu uso. MÉTODOS: As amostras selecionadas de 4 Centros de Tratamento de Efluentes Domiciliários foram analisadas de acordo com as normas da "Environmental Protection Agenty", Organização Mundial da Saúde e do "Standard Methods for Examination of Water and Wastewater", além de algumas classificações. RESULTADOS: Somente 2 das 6 amostras de semi-sólidos analisadas continham ovos de Ascaris lumbricoides não viáveis. Das 10 amostras líquidas, somente 2 não continham ovos, enquanto as restantes continham patógenos das categorias I (Giardia sp., Hymenolepis diminuta e Enterobius

  14. Sociologie de l’alimentation : les cinq portes de l’entrée par les familles 1

    OpenAIRE

    Belorgey, Nicolas

    2012-01-01

    La sociologie de l’alimentation s’est peu à peu autonomisée par rapport à la sociologie de la famille. Les deux corpus ne tirent pas aujourd'hui tout le parti de ce qu’ils ont en commun. Ce texte propose un inventaire des courants de recherche, sociologiques ou non, qui s’intéressent à la famille et qui fournissent des éléments théoriques mobilisables ou objectivables pour la sociologie de l’alimentation. On peut identifier ainsi cinq grands courants : l’approche statistique fondée sur les ca...

  15. Courant Number and Mach Number Insensitive CE/SE Euler Solvers

    Science.gov (United States)

    Chang, Sin-Chung

    2005-01-01

    It has been known that the space-time CE/SE method can be used to obtain ID, 2D, and 3D steady and unsteady flow solutions with Mach numbers ranging from 0.0028 to 10. However, it is also known that a CE/SE solution may become overly dissipative when the Mach number is very small. As an initial attempt to remedy this weakness, new 1D Courant number and Mach number insensitive CE/SE Euler solvers are developed using several key concepts underlying the recent successful development of Courant number insensitive CE/SE schemes. Numerical results indicate that the new solvers are capable of resolving crisply a contact discontinuity embedded in a flow with the maximum Mach number = 0.01.

  16. The Lie group of automorphisms of a Courant algebroid and the moduli space of generalized metrics

    OpenAIRE

    Rubio, Roberto; Tipler, Carl

    2016-01-01

    We endow the group of automorphisms of an exact Courant algebroid over a compact manifold with an infinite dimensional Lie group structure modelled on the inverse limit of Hilbert spaces (ILH). We prove a slice theorem for the action of this Lie group on the space of generalized metrics. As an application, we show that the moduli space of generalized metrics is stratified by ILH submanifolds. Finally, we relate the moduli space of generalized metrics to the moduli space of usual metrics.

  17. Star products on graded manifolds and α′-corrections to Courant algebroids from string theory

    Energy Technology Data Exchange (ETDEWEB)

    Deser, Andreas, E-mail: andreas.deser@itp.uni-hannover.de [Institut für Theoretische Physik and Riemann Center for Geometry and Physics, Leibniz Universität Hannover, Hannover (Germany)

    2015-09-15

    Courant algebroids, originally used to study integrability conditions for Dirac structures, have turned out to be of central importance to study the effective supergravity limit of string theory. The search for a geometric description of T-duality leads to Double Field Theory (DFT), whose gauge algebra is governed by the C-bracket, a generalization of the Courant bracket in the sense that it reduces to the latter by solving a specific constraint. Recently, in DFT deformations of the C-bracket and O(d, d)-invariant bilinear form to first order in the closed string sigma model coupling, α′ were derived by analyzing the transformation properties of the Neveu-Schwarz B-field. By choosing a particular Poisson structure on the Drinfel’d double corresponding to the Courant algebroid structure of the generalized tangent bundle, we are able to interpret the C-bracket and bilinear form in terms of Poisson brackets. As a result, we reproduce the α′-deformations for a specific solution to the strong constraint of DFT as expansion of a graded version of the Moyal-Weyl star product.

  18. Effects of PAR and UV-B radiation on herbal yield, bioactive compounds and their antioxidant capacity of some medicinal plants under controlled environmental conditions.

    Science.gov (United States)

    Manukyan, Artur

    2013-01-01

    Photosynthetically active radiation (PAR) and Ultraviolet B (UV-B) radiation are among the main environmental factors acting on herbal yield and biosynthesis of bioactive compounds in medicinal plants. The objective of this study was to evaluate the influence of biologically effective UV-B light (280-315 nm) and PAR (400-700 nm) on herbal yield, content and composition, as well as antioxidant capacity of essential oils and polyphenols of lemon catmint (Nepeta cataria L. f. citriodora), lemon balm (Melissa officinalis L.) and sage (Salvia officinalis L.) under controlled greenhouse cultivation. Intensive UV-B radiation (2.5 kJ m(-2)  d(-1) ) influenced positively the herbal yield. The essential oil content and composition of studied herbs were mainly affected by PAR and UV-B radiation. In general, additional low-dose UV-B radiation (1 kJ m(-2) d(-1) ) was most effective for biosynthesis of polyphenols in herbs. Analysis of major polyphenolic compounds provided differences in sensitivity of main polyphenols to PAR and UV-B radiation. Essential oils and polyphenol-rich extracts of radiated herbs showed essential differences in antioxidant capacity by the ABTS system. Information from this study can be useful for herbal biomass and secondary metabolite production with superior quality under controlled environment conditions. © 2012 Wiley Periodicals, Inc. Photochemistry and Photobiology © 2012 The American Society of Photobiology.

  19. Controle d'attitude d'un lanceur en phase atmospherique approche par applications gardiennes

    Science.gov (United States)

    Dubanchet, Vincent

    In a first phase, the modelling process underlines the presence of highly time varying parameters during the ascent, due to a fast mass variation along with propellant consumption. Linearizing the dynamical equations at six main flight instants yields linear time invariant models to be considered during control design. Each of them is to be stabilized by one control law, while respecting given specifications. The synthesis becomes even more complex when the bending modes are taken into account. Moreover, scheduling appears necessary to deal with the time variations. Indeed it is shown that no single gain setting is able to respect all the specifications along the trajectory. Furthermore, increasing complexity when modelling a whole launch vehicle pushes one to consider the model's errors and uncertainties. They represent a major issue in this study since it is asked to ensure the nominal performances in a robust fashion. Owing to their properties, guardian maps appear to be the most suitable tool to deal with such a problem of scheduling with robust performances. In light of this, the development of synthesis methods based on guardian maps is the main contribution of the project. It appears that actual state of the art in this field is focused on theoretical issues, whereas practical ones could be improved. Two approches are presented in the memoire. The first one is based on a graphical approach consisting in drawing the vanishing locus of guardian maps. A program using image analysis techniques is devised to check automatically which gain settings satisfy the constraints. The second one is based on an optimisation procedure involving guardian maps. Starting with the open loop system, the iterative process proposed ends up with a satisfactory gain setting for the closed-loop. These methods are tried and tested for the launch vehicle, with specifications from ASTRIUM-ST. Their practical application is motivated by the system complexity, the different kinds of

  20. Interfaces adaptatives Adaptation dynamique \\`a l'utilisateur courant

    CERN Document Server

    Simonin, Jérôme

    2007-01-01

    We present a survey of recent research studies of the implementation of adaptive user models in human-computer interaction. A classification of research directions on adaptive user interfaces is first proposed; it takes account of the user characteristics that are modelled, the distribution of initiative and control of the system evolution between user and system, and the role of dynamic adaptation. Then, a few representative research studies are briefly presented to illustrate this classification. In the conclusion, some major issues regarding the utility and usability of adaptive user interfaces and the design of an appropriate methodology for assessing the ergonomic quality of this new form of interaction are mentioned.

  1. Applied Technologies and Prospects of Conformance Control Treatments in China Technologies et perspectives pour l’amélioration du profil de balayage dans les champs pétroliers matures produits par injection d’eau en Chine

    Directory of Open Access Journals (Sweden)

    Liu Yuzhang

    2010-02-01

    Full Text Available China is the largest user of chemical-based conformance control treatments and a series of technologies have been successfully developed and deployed in recent years. This paper first shows the milestones of development and application of conformance control technologies in China. Then integrated conformance control technologies are reviewed followed by the lessons we have learned, and then a few major specific conformance control technologies are addressed, including tracer injection and channels explanation, potentiometric testing to identify areal sweep efficiency, Pressure Index (PI decisionmaking technology to select well candidate, complementary decision-making technology to select well candidate and design application parameters, and major chemicals for in-depth fluid diversion technologies. In addition, this paper also describes the principles and applications of some promising technologies of combined chemical-based conformance treatment with other EOR/IOR process, including the combination technology of surfactant and water shutoff, profile control and mini-scale surfactant flooding, acid treatment and profile control treatment. Finally, this paper summarizes the problems and challenges faced by mature water flooded oilfields in China. Based on recent well tests, tracer testing and interpretation, and previous water control treatment experience, it appears that channels or high permeability streaks are common in mature water flooded oilfields. Some research directions and promising technologies are suggested. La Chine est le plus grand utilisateur de méthodes chimiques pour l’amélioration du profil de balayage de l’eau pour l’exploitation des champs pétroliers matures. Toute une série de technologies y ont d’ailleurs été créées et déployées avec succès ces dernières années. Cet article commence par présenter les étapes du développement et de la mise en place de ces technologies de contrôle de profil en Chine

  2. Comparaison de lois de commande. Régulation numérique de courant dans l'association convertisseur-machine

    Science.gov (United States)

    Kalinowski, D.; Bergmann, C.

    1991-05-01

    This article deals with a comparison of different numerical command laws, as regards dynamical characteristics and robustness, for direct current motor control. The power converter, which uses bi-directional switches, connect directly the three-phase source to the servo-motor. Simulations, compared with experimental results, show the variations of the dynamical characteristics regarding perturbations, that is to say: model parameters are not constant, the converter is not linear, and there are numerical saturations. Cet article présente différentes stratégies de commande, sur un critère de rapidité/robustesse, pour une régulation numérique du courant dans un servo-moteur à courant continu. Le convertisseur de puissance, à la base de la structure, est constitué d'interrupteurs bidirectionnels et réalise l'alimentation directe de la charge à travers un réseau triphasé. Les simulations, confrontées aux résultats expérimentaux, montrent la très nette dépendance des caractéristiques dynamiques finales vis-à-vis des éléments perturbateurs à savoir : la variation des paramètres constituants le modèle, les non-linéarités des convertisseurs et les saturations numériques.

  3. Direct digital control of furnaces irradiated in nuclear reactors; Surveillance et regulation multiplexee par calculateur numerique de fours irradies

    Energy Technology Data Exchange (ETDEWEB)

    Joumard, R. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1969-07-01

    An experimental direct digital control system has been realised in the 'C.E.N.G.', in order to verify that a computer makes easier the control of the experiments done in the nuclear reactors and to solve the theoretical and technical difficulties. The regulation is applied to thermal processes. The sampled data systems theory permits to choose the type of an efficient and simple digital compensator, and to establish a diagram which gives the values of the correcting parameters (obtained by minimizing the difference between the output and the input when perturbations occur). The programme execute, in simultaneity, supervision and regulation. Complex possibilities of printing out measures and alarms existed. The computer works out an incremental correction which makes step motors to turn. These motors act on the heating organs. The theoretical values and answers have been confirmed. The accuracy was limited essentially by the input quantification (1/1000 th). The comfort of such a system has been noticeable. (author) [French] Une installation de controle numerique direct fut realisee a titre experimental au C.E.N.G pour verifier qu'un ordinateur rendait plus aisee l'exploitation des experiences faites en pile nucleaire et pour degager les difficultes theoriques et techniques. La regulation s'applique a des processus thermiques. La theorie des systemes echantillonnes a permis de choisir un type de correcteur numerique simple et efficace et d'etablir un abaque qui donne les valeurs des parametres correcteurs minimisant les ecarts enregistres entre la reponse et la consigne en presence de perturbations. Le programme effectuait simultanement de la surveillance et de la regulation. Une restitution complexe des informations et des alarmes sur machine a ecrire etait possible. Le calculateur elaborait une correction incrementielle qui faisait tourner des moteurs pas a pas, lesquels commandaient les organes de puissance de chauffage. Les valeurs

  4. Edge localized modes control by resonant magnetic perturbations; Controle des instabilites de bord par perturbations magnetiques resonantes

    Energy Technology Data Exchange (ETDEWEB)

    Nardon, E

    2007-10-15

    The present work is dedicated to one of the most promising methods of control of the ELMs (Edge Localized Modes), based on a system of coils producing Resonant Magnetic Perturbations (RMPs). Our main objectives are, on the one hand, to improve the physical understanding of the mechanisms at play, and on the other hand to propose a concrete design of ELMs control coils for ITER. In order to calculate and analyze the magnetic perturbations produced by a given set of coils, we have developed the ERGOS code. The first ERGOS calculation was for the DIII-D ELMs control coils, the I-coils. It showed that they produce magnetic islands chains which overlap at the edge of the plasma, resulting in the ergodization of the magnetic field. We have then used ERGOS for the modelling of the experiments on ELMs control using the error field correction coils at JET and MAST. In the case of JET, we have shown the existence of a correlation between the mitigation of the ELMs and the ergodization of the magnetic field at the edge, in agreement with the DIII-D result. In order to design the ELMs control coils for ITER we have used ERGOS intensively, taking the case of the DIII-D I-coils as a reference. Three candidate designs came out, which we presented at the ITER Design Review, in 2007. Recently, the ITER management decided to provide a budget for building ELMs control coils, the design of which remains to be chosen between two of the three options that we proposed. Finally, in order to understand better the non-linear magnetohydrodynamics phenomena taking place in ELMs control by RMPs, we performed numerical simulations, in particular with the JOREK code for a DIII-D case. The simulations reveal the existence of convection cells induced at the edge by the magnetic perturbations, and the possible screening of the RMPs in presence of rotation.

  5. Ultrasonic testing of canning tubes in stainless steel of the EL 4 reactor; Controle par ultrasons des tubes de gaine en acier inoxydable du reacteur EL 4

    Energy Technology Data Exchange (ETDEWEB)

    Prot, A.; Monnier, P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-01

    From all the methods possible for controlling thin cans the one chosen, for numerous reasons, vas that making use of ultrasonic techniques. A method has been developed which should make it possible to carry out a rapid and efficient industrial control of canning tubes, The reasons for the choice of the ultrasonic method are given in detail, together with the principles of the method and the actual control parameters. In the present state of our research, it should be possible to control at least 50 000 tubes a year. Improvements brought about in the details of the control technique itself should make it possible to increase this rate considerably. (authors) [French] Parmi toutes les methodes possibles de controle des gaines minces, le procede retenu pour de multiples raisons a ete celui faisant appel a la technique des ultrasons. Une methode a ete mise au point qui doit permettre un controle industriel rapide et efficace des tubes de gaine. Sont exposes en detail, les raisons du choix de la methode par ultrasons, les principes de cette methode et les parametres du controle proprement dit. Dans l'etat actuel de nos etudes la cadence devrait permettre le controle de 50000 tubes par an au minimum. Des ameliorations de detail portant sur la technique de controle elle-meme, doivent permettre d'accelerer tres notablement cette cadence. (auteurs)

  6. Generalized Courant-Snyder theory for charged-particle dynamics in general focusing lattices.

    Science.gov (United States)

    Qin, Hong; Davidson, Ronald C; Chung, Moses; Burby, Joshua W

    2013-09-06

    The Courant-Snyder (CS) theory for one degree of freedom is generalized to the case of coupled transverse dynamics in general linear focusing lattices with quadrupole, skew-quadrupole, dipole, and solenoidal components, as well as torsion of the fiducial orbit and variation of beam energy. The envelope function is generalized into an envelope matrix, and the phase advance is generalized into a 4D sympletic rotation. The envelope equation, the transfer matrix, and the CS invariant of the original CS theory all have their counterparts, with remarkably similar expressions, in the generalized theory.

  7. Étude de la répartition des courants de Foucault pulsés dans une configuration de contrôle non destructif

    Science.gov (United States)

    Bour, J.-C.; Zubiri, E.; Vasseur, P.; Billat, A.

    1996-01-01

    Adjusting an experimental device of control by eddy-currents in sinusoidal or pulse mode requires the optimization of a certain number of parameters. Generally, the implementation of the latter operations is long and delicate, since it is issued of many compromises. The simulation study presented in this paper can be considered as a qualitative help because it specifies the the behaviour of the different parameters implied in the adjustment of the experimental defect reserach. First, we prove the influence of the lift-off on the defect detention's sensitivity of the flat sensor we used. Next we give prominence to the fact, in practice, we cannot disregard the geometry of the sensor, as we could do in theory. This result will lead us to define a family of curves showing the evolution of the depth of penetration as a function of the pulse's duration for different coil radii. We also determine the maximal sensor's sensitivity area, with the aim of locating the defect detected in the plate with precision. Finally, we prove the dependency between the true value of the depth of penetration and the thickness of the considered metallic plate. This very important result should equally be taken under consideration in the case of a real control. La mise au point d'un dispositif expérimental de contrôle par courants de Foucault, en mode sinusoïdal ou impulsionnel, nécessite l'optimisation d'un certain nombre de paramètres. Cette optimisation est généralement longue et délicate à exécuter en pratique puisqu'elle résulte de plusieurs compromis. L'étude en simulation présentée ici peut être considéré comme une aide plutôt qualitative, puisqu'elle précise le comportement des différents paramètres impliqués dans la mise en point de la recherche expérimentale de défauts. Nous montrons dans un premier temps l'influence de la distance capteur-cible sur la sensibilité du capteur à détecter un défaut. Nous mettons ensuite en évidence le fait de la g

  8. Calculation of pole profiles for the design of particle accelerators magnets. Equivalent current and conformal representation methods; Calcul de profils polaires destines a des aimants d'accelerateurs de particules. Methode des courants et methode des transformations conformes

    Energy Technology Data Exchange (ETDEWEB)

    Jaidane, S. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1968-04-01

    These two methods allow the determination of the shape of the poles in magnets, for a given field distribution in the air-gap. First method: The principle of the method consists to create the desired law of field by means of current sheets in which one can adjust the density given in a polynomial form. For the right distribution of these currents, the equipotential corresponding to the magnetic potential of the excitation coils is calculated. The pole profile of the H or C magnet identified with this equipotential line will finally take the place of the distribution of the current sheets used in the calculation. Steel permeability is assumed to be infinite and Foucault current effects are neglected in the case of variable fields. Second method: It consists to find a conformal representation that maps the pole profile plane upon the upper half of another plane where the equipotentials are two half straight lines, and where the field problems are easier to solve. Steel permeability is also considered to be infinite and the coils far from the pole faces. This known method has been applied to be compared with the first one. (author) [French] Ces deux methodes consistent a determiner la forme des pieces polaires d'aimants pour une distribution de champ determinee a l'avance dans l'entrefer. Premiere methode: Le principe de la methode consiste a creer la loi de champ desiree par l'intermediaire de nappes de courant dont on peut ajuster la densite exprimee sous une forme polynominale. Pour une distribution convenable de ces courants, on calcule l'equipotentielle correspondant au potentiel magnetique des bobines d'excitation. Le profil polaire d'un aimant en H ou C identifie a l'equipotentielle se substitue finalement a la repartition des nappes de courant utilisee dans la methode de calcul. La permeabilite de l'acier est supposee infinie et les courants de Foucault sont negliges dans le cas des champs variables. Seconde methode

  9. The close-range gamma-graphy control - {gamma}-prox; Le controle par gammagraphie de proximite - {gamma}-prox

    Energy Technology Data Exchange (ETDEWEB)

    Blettner, A. [Institut de Soudure, 93 - Villepinte (France)

    2011-01-15

    This work deals with the {gamma}-prox: a modular device for proximity gamma-graphy. The dose rates obtained between the standard collimator and the {gamma}-prox are compared. A study, among the tools developed by the Welding Institute Industry, to estimate the occupational dosimetry is presented in order to determine the dose limits for a welding control of 255 mm diameter and 5 mm thickness, as well as the calculation of the signalling distances. (O.M.)

  10. Intégration d'une batterie à circulation pour lisser la puissance quotidienne fournie par une hydrolienne sur un réseau îloté

    OpenAIRE

    ZHOU, Zibhin; SCUILLER, Franck; Charpentier, Jean-Frederic; Benbouzid, Mohamed

    2014-01-01

    En raison de la prévisibilité des courants marins, les hydroliennes apparaissent a priori comme très attractives en tant que source d’électricité future pour les zones côtières isolées et les îles éloignées situées à proximité de sites à forts courants comme les îles du Ponant au large du Finistère. Cependant la puissance produite par une hydrolienne varie considérablement avec la vitesse des courants marins pendant une journée. Cet article propose d’utiliser une batterie à circulation ou « f...

  11. De Par en Par (Wide Open), 1993.

    Science.gov (United States)

    De Par en Par, 1993

    1993-01-01

    This document consists of the four issues of the serial "De Par en Par" published during 1993. This serial provides lessons in Spanish for elementary school children. It is written by bilingual education teachers for use in the bilingual classroom. The magazine bases itself on the K-6 curriculum and offers a variety of activities for classroom…

  12. Effectiveness of the EMPOWER-PAR Intervention in Improving Clinical Outcomes of Type 2 Diabetes Mellitus in Primary Care: A Pragmatic Cluster Randomised Controlled Trial.

    Science.gov (United States)

    Ramli, Anis Safura; Selvarajah, Sharmini; Daud, Maryam Hannah; Haniff, Jamaiyah; Abdul-Razak, Suraya; Tg-Abu-Bakar-Sidik, Tg Mohd Ikhwan; Bujang, Mohamad Adam; Chew, Boon How; Rahman, Thuhairah; Tong, Seng Fah; Shafie, Asrul Akmal; Lee, Verna K M; Ng, Kien Keat; Ariffin, Farnaza; Abdul-Hamid, Hasidah; Mazapuspavina, Md Yasin; Mat-Nasir, Nafiza; Chan, Chun W; Yong-Rafidah, Abdul Rahman; Ismail, Mastura; Lakshmanan, Sharmila; Low, Wilson H H

    2016-11-14

    The chronic care model was proven effective in improving clinical outcomes of diabetes in developed countries. However, evidence in developing countries is scarce. The objective of this study was to evaluate the effectiveness of EMPOWER-PAR intervention (based on the chronic care model) in improving clinical outcomes for type 2 diabetes mellitus using readily available resources in the Malaysian public primary care setting. This was a pragmatic, cluster-randomised, parallel, matched pair, controlled trial using participatory action research approach, conducted in 10 public primary care clinics in Malaysia. Five clinics were randomly selected to provide the EMPOWER-PAR intervention for 1 year and another five clinics continued with usual care. Patients who fulfilled the criteria were recruited over a 2-week period by each clinic. The obligatory intervention components were designed based on four elements of the chronic care model i.e. healthcare organisation, delivery system design, self-management support and decision support. The primary outcome was the change in the proportion of patients achieving HbA1c pressure, lipid profile, body mass index and waist circumference. Intention to treat analysis was performed for all outcome measures. A generalised estimating equation method was used to account for baseline differences and clustering effect. A total of 888 type 2 diabetes mellitus patients were recruited at baseline (intervention: 471 vs. 417). At 1-year, 96.6 and 97.8% of patients in the intervention and control groups completed the study, respectively. The baseline demographic and clinical characteristics of both groups were comparable. The change in the proportion of patients achieving HbA1c target was significantly higher in the intervention compared to the control group (intervention: 3.0% vs. -4.1%, P < 0.002). Patients who received the EMPOWER-PAR intervention were twice more likely to achieve HbA1c target compared to those in the control group

  13. Efecto en la respuesta temporal del parámetro ajustable del control adaptativo con modelo de referencia de primer orden

    OpenAIRE

    Vargas Tamani, Bruno; Universidad Nacional Mayor de San Marcos

    2014-01-01

    Se presentan los resultados experimentales del control adaptativo con modelo de referencia, de una planta que de acuerdo a su identificación previamente realizada se puede representar como un sistema de primer orden. Se elige como modelo de referencia también uno de primer orden con ganancia unitaria. Se realiza el análisis del controlador adaptativo, justificándose que su desempeño en lazo cerrado dependerá de la elección adecuada de un parámetro escalar ajustable. Se implementa el controlad...

  14. Commande d'un actionneur électrique par communication sans fil Zigbee

    OpenAIRE

    Llibre, Jean-François

    2010-01-01

    CNR IUT Angers 2010; Nous présentons ici un asservissement de position en sans fil d'un moteur à courant continu. La consigne élaborée au niveau d'un PC distant est envoyée via un module Zigbee à un autre module Zigbee, qui lui relié au moteur à commander, renvoie la mesure de la position. La commande numérique mise en œuvre doit être suffisamment robuste et la période d'échantillonnage judicieusement choisie pour s'affranchir des contraintes imposées par la communication hertzienne. Nous uti...

  15. Optimal Parameter Updating in Assisted History Matching Using Streamlines as a Guide Mise à jour optimale des paramètres dans un processus de calage d’historique en s’aidant des lignes de courants

    Directory of Open Access Journals (Sweden)

    Kazemi A.

    2013-05-01

    paramètres n’ont pas la même importance, et identifier les zones du réservoir où les paramètres doivent être mis à jour est un défi. Dans cette étude, nous étudions les méthodes de calage d’historique en nous concentrant sur les sous-ensembles des paramètres du modèle, et nous utilisons les lignes de courant pour choisir les zones du modèle qui doivent être modifiées. Nous identifions les régions du réservoir affectant des puits particuliers et nous y ajustons les propriétés du réservoir (fraction de roche réservoir et perméabilité. Les changements sont réalisés à partir de la méthode des points pilotes associée à un algorithme d’optimisation par voisinage. Nous appliquons cette méthodologie au champ Nelson (North Sea, UK pour lequel les prévisions de production dépendent de l’incertitude sur la distribution des argiles. Le champ est divisé en régions sur la base des performances des plus mauvaises prédictions aux puits. Nous forçons les modèles de réservoir à respecter les historiques de production pour améliorer les taux de production. Les régions nécessitant des changements sont suffisamment distinctes pour que nous puissions les modifier une à la fois. Nous comparons également nos résultats à une approche plus ad hoc qui implique la modification de toute la zone autour du puits. Nous observons que, pour les puits d’intérêt, l’approche guidée par les lignes de courant amène à une amélioration de 70 % du calage des données de production comparé au modèle de départ et à une réduction d’environ 40 % des écarts pour les prévisions. Cette amélioration est deux fois plus élevée que celle apportée par une modification des propriétés tout autour du puits.

  16. Par-tjek Manualen

    DEFF Research Database (Denmark)

    Trillingsgaard, Tea; Due, Mattias Stølen; Nørr Fentz, Hanne;

    Par-tjek er et tilbud til par, der ønsker at styrke forholdet og forebygge vanskeligheder. Et Par-tjek består af et indledende online spørgeskema efterfulgt at to samtaler med en psykolog eller lignende vejleder. Ved den sidste samtale modtager parret en personlig feedback-rapport, som de kan...

  17. Explicit Von Neumann Stability Conditions for the c-tau Scheme: A Basic Scheme in the Development of the CE-SE Courant Number Insensitive Schemes

    Science.gov (United States)

    Chang, Sin-Chung

    2005-01-01

    As part of the continuous development of the space-time conservation element and solution element (CE-SE) method, recently a set of so call ed "Courant number insensitive schemes" has been proposed. The key advantage of these new schemes is that the numerical dissipation associa ted with them generally does not increase as the Courant number decre ases. As such, they can be applied to problems with large Courant number disparities (such as what commonly occurs in Navier-Stokes problem s) without incurring excessive numerical dissipation.

  18. Modélisation vectorielle et commande de machines polyphasées à pôles lisses alimentées par onduleur de tension

    OpenAIRE

    SEMAIL, Eric; Kestelyn, Xavier

    2010-01-01

    Ce chapitre traite de la modélisation et de la commande de machines électriques à plus de deux courants indépendants alimentées par onduleur de tension. Une approche vectorielle générale permettant de généraliser le concept de machine diphasée équivalente utilisé pour les machines triphasées est présentée et appliquée au cas d’une machine synchrone prototype à aimants permanents cinq phases alimentée soit par 5 bras d’onduleurs.

  19. Étude de la réponse en courant de détecteurs silicium opérés en environnement de très hautes radiations

    CERN Document Server

    Roy, Patrick

    Ce travail se situe dans le cadre de l'expérience ATLAS auprès du Grand Collisionneur Hadronique (LHC) qui sera construit au CERN. Il consiste en l'étude de la réponse en courant de détecteurs au silicium de grande surface, irradiés par des neutrons ou des protons de façon à reproduire l'environnement de hautes radiations auquel le détecteur ATLAS devra faire face. A l'aide d'une modélisation du transport des charges produites par le passage de particules alpha ou bêta dans un détecteur au silicium et d'un ensemble de données expérimentales, il est possible d'obtenir certaines caractéristiques électriques importantes des détecteurs. Ces caractéristiques sont: la concentration effective de dopants (ce qui détermine la tension de désertion d'un semi-conducteur), le piégeage des porteurs de charge (donnant l'efficacité de la collection des charges), et les mobilités des électrons et des trous (influençant le temps nécessaire à la collection des charges). En connaissant l'évolution de...

  20. Archimedes in the 21st Century : World Conference at the Courant Institute of Mathematical Sciences

    CERN Document Server

    2017-01-01

    This book is a collection of papers presented at the “Archimedes in the 21st Century” world conference, held at the Courant Institute of Mathematical Sciences in 2013. This conference focused on the enduring and continuing influence of Archimedes in our modern world, celebrating his centuries of influence on mathematics, science, and engineering.  Archimedes planted the seeds for a myriad of seminal ideas that would grow over the ages. Each chapter surveys the growth of one or more of these seeds, and the fruit that they continue to bear to this day. The conference speakers contributing to this book are actively involved in STEM fields whose origins trace back to Archimedes, many of whom have conducted and published research that extends Archimedes’ work into the 21st century. The speakers are not historians, so while historical context is provided, this book is uniquely focused on the works themselves as opposed to their history.   The breadth and depth of Archimedes’ influence will inspire, deligh...

  1. Evaluer les apprentissages dans le cadre d'une pédagogie par projet

    OpenAIRE

    Scheffers, Patricia; Leduc, Diane; Le Coguiec, Eric

    2015-01-01

    Avant d'être l'affaire des courants de l'éducation nouvelle, la pédagogie par projet était présente dans les discours de formation d'architecture italienne dès le 16e siècle. Aujourd'hui, elle s'inscrit parmi les multiples approches d'une pédagogie différenciée et est utilisée dans plusieurs disciplines, des arts aux sciences, en passant par l'ingénierie et l'administration. Plusieurs écrits existent à son sujet, mais peu s'attardent aux pratiques d'évaluation des apprentissages qu'elle génèr...

  2. Performance testing of passive autocatalytic recombiners (PARs)

    Energy Technology Data Exchange (ETDEWEB)

    Blanchat, T. [Sandia National Laboratories, Albuquerque, NM (United States); Malliakos, A. [U.S. Nuclear Regulatory Commission, Washington, DC (United States)

    1997-03-01

    Passive autocatalytic recombiners (PARs) have been under consideration in the U.S. as a combustible gas control system in advanced light water reactor (ALWR) containments for design basis and severe accidents. PARs do not require a source of power. Instead they use palladium or platinum as a catalyst to recombine hydrogen and oxygen gases into water vapor upon contact with the catalyst. Energy from the recombination of hydrogen with oxygen is released at a relatively slow but continuous rate into the containment which prevents the pressure from becoming too high. The heat produced creates strong buoyancy effects which increases the influx of the surrounding gases to the recombiner. These natural convective flow currents promote mixing of combustible gases in the containment. PARs are self-starting and self-feeding under a very wide range of conditions. The recombination rate of the PAR system needs to be great enough to keep the concentration of hydrogen (or oxygen) below acceptable limits. There are several catalytic recombiner concepts under development worldwide. The USNRC is evaluating a specific design of a PAR which is in an advanced stage of engineering development and has been proposed for ALWR designs. Sandia National laboratories (SNL), under the sponsorship and the direction of the USNRC, is conducting an experimental program to evaluate the performance of PARs. The PAR will be tested at the SURTSEY facility at SNL. The test plan currently includes the following experiments: experiments will be conducted to define the startup characteristics of PARs (i.e., to define what is the lowest hydrogen concentration that the PAR starts recombining the hydrogen with oxygen); experiments will be used to define the hydrogen depletion rate of PARs as a function of hydrogen concentration; and experiments will be used to define the PAR performance in the presence of high concentrations of steam. (author)

  3. Installation solaire de production hydrogène par l'électrolyse de vapeur d'eau

    OpenAIRE

    Miri, Rachid; Mraoui, Abdelhamid

    2007-01-01

    International audience; Cette étude porte sur la conception d'une installation de production d'hydrogène par électrolyse de vapeur d'eau à hautes températures (700-1000°C) dont les ressources énergétiques sont solaires : l'alimentation en vapeur d'eau est assurée par une centrale solaire à concentration et le courant électrique est fourni par des cellules Photovoltaïques. La simulation numérique de la production d'hydrogène, pour l'installation proposée, est faite en se basant sur les équatio...

  4. FooPar

    DEFF Research Database (Denmark)

    Hargreaves, F. P.; Merkle, D.

    2013-01-01

    We present FooPar, an extension for highly efficient Parallel Computing in the multi-paradigm programming language Scala. Scala offers concise and clean syntax and integrates functional programming features. Our framework FooPar combines these features with parallel computing techniques. Foo...

  5. Erreurs induites par l’utilisation d’un capteur de grande taille, un ceptomètre par exemple, pour calculer un coefficient d’extinction à partir de la mesure des rayonnements transmis sous une culture

    OpenAIRE

    Tournebize, Régis; Cornet, Denis; BONHOMME, Raymond

    2010-01-01

    L’estimation des rayonnements solaires interceptés par une culture est une donnée devenue courante en agronomie pour évaluer sa production. Cette estimation est le plus souvent faite à partir de la seule mesure de l’indice foliaire LAI et l’utilisation d’un coefficient d’extinction des rayonnements K. Ce coefficient K est souvent évalué à partir de mesures ponctuelles de l’indice foliaire et de la fraction des rayonnements solaires transmis sous une culture TR, en utilisant la for...

  6. Chromatographie à contre-courant simulé : développements et perspectives From Batch Elution to Simulated Countercurrent Chromatography

    Directory of Open Access Journals (Sweden)

    Hotier G.

    2006-11-01

    Full Text Available Les applications industrielles et le principe de fonctionnement du procédé de séparation en lit mobile ou en contre-courant simulé sont brièvement rappelés. La réalisation pratique de trois unités pilotes fonctionnant selon ce principe mais employant une technologie innovante est présentée. Cette mise en oeuvre particulière permet de tester par un simple changement de paramètres de contrôle toutes les configurations possibles de l'unité. Cet appareillage particulièrement souple est donc véritablement multitâche, ce qui permet en plus des applications en pétrochimie son emploi dans les domaines de la pharmacie, de la chimie fine et des bioséparations, où existe un grand nombre d'applications potentielles très différentes les unes des autres. Deux exemples de séparations réalisées à l'Institut Français du Pétrole sont succinctement présentés. La conclusion de ces essais est que la modélisation de ces procédés est indispensable pour pouvoir opérer correctement ce type d'unité. Une comparaison entre la chromatographie d'élution et cette technique de séparation ainsi que quelques propositions d'amélioration du procédé permettront au lecteur de se faire une idée sur l'extension potentielle de ce type de séparation. Distillation is and will for quite a long time remain the main separation technique in the refining, petrochemical and chemical industries. Some separations however cannot be performed by distillation when the products to be separated are subject to thermal degradation or when their boiling points are too close. Under these circumstances adsorption or chromatographic processes are interesting alternatives. In the 1960's and in the early 1970's the technology of the simulated moving bed, the UOP rotary valve and the application of these devices to separation processes were introduced by UOP. Nowadays simulated moving bedor countercurrentseparation processes may be found in the petrochemical industry

  7. Aplicación de algoritmos de control clásico, adaptable y robusto a sistemas dinámicos de parámetros variables;Classic, adaptable and robust control algorithm application, to variant parameter dynamic system.

    Directory of Open Access Journals (Sweden)

    Orlando – Regalón Anias

    2012-11-01

    Full Text Available Existen múltiples sistemas dinámicos cuyos modelos matemáticos se caracterizan por ser de primer orden yparámetros variables con el tiempo. En estos casos las herramientas clásicas no siempre logran un sistema decontrol que sea estable, posea un buen desempeño dinámico y rechace adecuadamente las perturbaciones, cuandoel modelo de la planta se desvía del nominal, para el cual se realizó el diseño.En este trabajo se evalúa elcomportamiento de tres estrategias de control en presencia de variación de parámetros. Estas son: control clásico,control adaptable y control robusto. Se realiza un estudio comparativo de las mismas en cuanto a complejidad deldiseño, costo computacional de la implementación y sensibilidad ante variaciones en los parámetros y/o presencia dedisturbios. Se llega a conclusiones que permiten disponer de criterios para la elección más adecuada, endependencia de los requerimientos dinámicos que la aplicación demande, así como de los medios técnicos de que sedisponga.Many dynamic systems have first order mathematic models, with time variable parameters. In these cases, theclassical tools do not satisfy at all control system stability, good performance and perturbation rejection, when theplant model differs from the nominal one, for which the controller was designed.In this article, three control strategiesare evaluated in parameter variations and disturbance presence. The strategies are the followings: classical control,adaptive control and robust control. A comparative study is carried out, taking into account the design complexity, thecomputational cost and the sensitivity. The obtained conclusions helps to provide the criterion to choose the mostadequate control strategy, according to the necessary dynamic, as well as the available technical means.

  8. suPAR

    DEFF Research Database (Denmark)

    Hodges, Gethin W; Bang, Casper N; Wachtell, Kristian;

    2015-01-01

    The fundamental role of inflammation in cardiovascular disease (CVD) has prompted interest in numerous biomarkers that detect subclinical levels of inflammation. Soluble urokinase plasminogen activator receptor (suPAR) is a novel biomarker that correlates significantly with cardiovascular events ...

  9. The receptor for urokinase-plasminogen activator (uPAR) controls plasticity of cancer cell movement in mesenchymal and amoeboid migration style

    Science.gov (United States)

    Taddei, Maria Letizia; Giannoni, Elisa; Laurenzana, Anna; Biagioni, Alessio; Chillà, Anastasia; Chiarugi, Paola; Fibbi, Gabriella; Rosso1, Mario Del

    2014-01-01

    The receptor for the urokinase plasminogen activator (uPAR) is up-regulated in malignant tumors. Historically the function of uPAR in cancer cell invasion is strictly related to its property to promote uPA-dependent proteolysis of extracellular matrix and to open a path to malignant cells. These features are typical of mesenchymal motility. Here we show that the full-length form of uPAR is required when prostate and melanoma cancer cells convert their migration style from the “path generating” mesenchymal to the “path finding” amoeboid one, thus conferring a plasticity to tumor cell invasiveness across three-dimensional matrices. Indeed, in response to a protease inhibitors-rich milieu, prostate and melanoma cells activated an amoeboid invasion program connoted by retraction of cell protrusions, RhoA-mediated rounding of the cell body, formation of a cortical ring of actin and a reduction of Rac-1 activation. While the mesenchymal movement was reduced upon silencing of uPAR expression, the amoeboid one was almost completely abolished, in parallel with a deregulation of small Rho-GTPases activity. In melanoma and prostate cancer cells we have shown uPAR colocalization with β1/β3 integrins and actin cytoskeleton, as well integrins-actin co-localization under both mesenchymal and amoeboid conditions. Such co-localizations were lost upon treatment of cells with a peptide that inhibits uPAR-integrin interactions. Similarly to uPAR silencing, the peptide reduced mesenchymal invasion and almost abolished the amoeboid one. These results indicate that full-length uPAR bridges the mesenchymal and amoeboid style of movement by an inward-oriented activity based on its property to promote integrin-actin interactions and the following cytoskeleton assembly. PMID:24681666

  10. Quality control summary report for the RFI/RI assessment of the submerged sediment core samples taken at Par Pond, Pond C, and L-Lake

    Energy Technology Data Exchange (ETDEWEB)

    Koch, J. II

    1996-12-01

    This report presents a summary of the sediment characterization performed under the direction of the Westinghouse Savannah River Company`s (WSRC) Environmental Protection Department/Environmental Monitoring Section (EPD/EMS) in support of Par Pond, Pond C, and L- Lake. This characterization will be a screening study and will enable the Environmental Sciences Section (ESS) to develop a defensible contaminants of concern list for more extensive characterization of the Par Pond, Pond C, and L-Lake.

  11. The receptor for urokinase-plasminogen activator (uPAR) controls plasticity of cancer cell movement in mesenchymal and amoeboid migration style.

    Science.gov (United States)

    Margheri, Francesca; Luciani, Cristina; Taddei, Maria Letizia; Giannoni, Elisa; Laurenzana, Anna; Biagioni, Alessio; Chillà, Anastasia; Chiarugi, Paola; Fibbi, Gabriella; Del Rosso, Mario

    2014-03-30

    The receptor for the urokinase plasminogen activator (uPAR) is up-regulated in malignant tumors. Historically the function of uPAR in cancer cell invasion is strictly related to its property to promote uPA-dependent proteolysis of extracellular matrix and to open a path to malignant cells. These features are typical of mesenchymal motility. Here we show that the full-length form of uPAR is required when prostate and melanoma cancer cells convert their migration style from the "path generating" mesenchymal to the "path finding" amoeboid one, thus conferring a plasticity to tumor cell invasiveness across three-dimensional matrices. Indeed, in response to a protease inhibitors-rich milieu, prostate and melanoma cells activated an amoeboid invasion program connoted by retraction of cell protrusions, RhoA-mediated rounding of the cell body, formation of a cortical ring of actin and a reduction of Rac-1 activation. While the mesenchymal movement was reduced upon silencing of uPAR expression, the amoeboid one was almost completely abolished, in parallel with a deregulation of small Rho-GTPases activity. In melanoma and prostate cancer cells we have shown uPAR colocalization with β1/β3 integrins and actin cytoskeleton, as well integrins-actin co-localization under both mesenchymal and amoeboid conditions. Such co-localizations were lost upon treatment of cells with a peptide that inhibits uPAR-integrin interactions. Similarly to uPAR silencing, the peptide reduced mesenchymal invasion and almost abolished the amoeboid one. These results indicate that full-length uPAR bridges the mesenchymal and amoeboid style of movement by an inward-oriented activity based on its property to promote integrin-actin interactions and the following cytoskeleton assembly.

  12. Detection of {alpha} particles using semiconductors. Application to the control of plutonium extraction; Detection des particules {alpha} par semiconducteurs application au controle de l'extraction du plutonium

    Energy Technology Data Exchange (ETDEWEB)

    Tanguy, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1968-03-01

    A study is made of a particles produced by thick sources, using either diffused junction or surface barrier semiconductor detectors for controlling continuously the plutonium extraction process. For this, a presenting apparatus is described in which the solutions to be analyzed flow in contact with the detector protected by a thin mica membrane. A method is described which gives a precise recording of the spectra and which thus allows the separation of two or more {alpha} emitters present in the same solution. This method has been applied to the measurement of {sup 239}Pu in the the presence of {sup 241}Am with an accuracy of {+-}5 per cent. In the second part of the report is considered the detection of plutonium in solutions of {beta} - {gamma} emitting fission products. Pile-up is reduced by using a fast amplification chain associated to totally depleted thin detectors. Under these conditions a few mg of {sup 239}Pu can be detected in solutions of fission products having an activity of 100 curies/liter. A method is given for discriminating {alpha} and {beta} particles, it is based on the difference in the collection times for the charges liberated by these particles in the detector. (author) [French] On etudie la detection de particules {alpha} issues de sources epaisses par detecteurs semiconducteurs a jonction diffusee ou a barriere de surface pour le controle continu du procede d'extraction du plutonium. A cet effet on decrit un appareil presentateur dans lequel les solutions a analyser circulent au contact du detecteur protege par une membrane mince de mica. On decrit une methode qui permet par le trace precis des spectres de separer deux ou plusieurs emetteurs {alpha} presents dans une meme solution. Cette methode a ete appliquee a la mesure du {sup 239}Pu en presence de {sup 241}Am avec une precision de {+-} 5 pour cent. Dans la deuxieme partie on traite de la detection du plutonium dans des solutions de produits de fission emetteurs {beta} and {gamma

  13. It's quicker "Par Avignon"!

    CERN Multimedia

    2005-01-01

    For a few years, the CERN Library has been receiving books from the University of Hanover sent via Avignon, at least that's what it says on the envelope. Such a detour would mean that parcels were travelling 720 km more than the distance separating Geneva and Hanover, which would be a very strange state of affairs. The explanation lies in a spelling mistake. The sender has been stamping parcels with a stamp that says "Par Avignon prioritaire" (first-class via Avignon) instead of "Par Avion prioritaire" (First Class Air Mail), a source of much amusement to the librarians!

  14. Essais sur la production de l'hydrogène solaire par électrolyse de l'eau

    OpenAIRE

    Ben Slama, Romdhane

    2007-01-01

    International audience; L'intérêt accru aux combustibles nécessite la diversification de leur approvisionnement, et ce, surtout à une époque où le prix du baril a doublé en trois ans pour atteindre un record de 75 $/baril. Ce papier présente un début de travail sur la production de l'hydrogène par électrolyse de l'eau. Le courant nécessaire est fourni par un module photovoltaïque. Les expériences ont permis de déterminer les paramètres à optimiser pour des meilleures performances : il s'agit ...

  15. Image analysis from surface scanning with an absolute eddy current coil; Analyse d`images obtenues par balayage de surface avec un capteur ponctuel courants de Foucault

    Energy Technology Data Exchange (ETDEWEB)

    Attaoui, P.

    1994-10-19

    The aim of this work is to implement processing and analysis tools applied to eddy current imaging. These cartographies are issued from steam generator tubes testing using an absolute coil. The first is to eliminate the perturbations due to probe lift-off changes which generate low frequency oscillations on the image. The principle of the processing is to rebuild a complete surface of the noise using only the points around the defect area. The geometric origin of these perturbations led to a model based on sinusoidal functions. The method consists of gradually decomposing the image into a sum of basic sinusoidal surfaces. In order to take into account all kind of cartographies (especially rolling zone) some preprocessing must be applied. The results obtained with this `cartography flattening`are satisfactory and the phase of analysis could begin with good condition of signal ratio. The second part of this work dealt with the choice and the perfection of image processing tools which would fit the most with the defect characterization. The aim of this characterization is to give the orientation and main size of the detected defect. A morphological skeleton representation has been chosen to illustrate the defect architecture and to allow sizing. A set of tools has been elaborated to obtain an (automatic) processing according to threshold. The results for single defect are satisfactory since the sizing error is around {+-} 25% and orientation is nearly always correctly given. The processing for area with several defects is more complex and new complementary research directions are proposed. (author).

  16. Modélisation par une approche à deux fluides des écoulements gaz liquide à contre-courant dans les colonnes à garnissages

    OpenAIRE

    Fourati, Manel

    2012-01-01

    Ce travail de thèse rentre dans le cadre du développement de modèles multi‐échelles de simulation de colonne d’absorption gaz‐liquide pour des applications de captage de CO2 en vue d’optimiser leur design. Il est le fruit d’une collaboration entre IFP Énergies nouvelles et l’Institut de Mécanique des Fluides de Toulouse. Les colonnes à garnissages représentent une technologie essentielle aux applications d’absorption gaz‐liquide. Dans les procédés de captage de CO2 aux amines, le solvant liqu...

  17. Aplicación de Antagonistas Microbianos para el Control Biológico de Moniliophthora roreri Cif & Par en Theobroma cacao L. Bajo Condiciones de Campo / Application of Microbial Antagonists for the Biological Control of Moniliophthora roreri Cif & Par in

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Villamil Carvajal

    2014-12-01

    Full Text Available Resumen. En Colombia, las pérdidas ocasionadas porMoniliophthora roreri Cif & Par en el cultivo de cacao continúansiendo considerables. El objetivo del presente estudio fue investigarla actividad antagónica de dos aislamientos autóctonos deTrichoderma sp. y uno de Bacillus sp. ante M. roreri, en condicionesde campo. Los tratamientos fueron: T1, hongo H5; T2, hongo H20;T3, bacteria B3 y T4, testigo. Se evaluó incidencia y severidadexterna e interna en los frutos. Los resultados de severidad externae interna mostraron que respecto al control la disminucióndel daño en los frutos fue del 19,5 y 11,2% en el T1, del 28 y19,5% en el T2 y del 13,5 y 8,5% en el T3, respectivamente condiferencias estadísticas a favor del T2. Se concluye que entre lostres antagonistas evaluados, el hongo H20 (Trichoderma sp. tieneel mayor potencial para el control de la moniliasis del cacao encondiciones de campo. / Abstract. In Colombia, the economic losses caused byMoniliophthora roreri Cif & Par in the cocoa cultivation continuebeing considerable. The objective of this study was to investigatethe antagonistic activity of two indigenous isolates of Trichodermasp and one of Bacillus sp. over M. roreri under field conditions. Thetreatments were: T1, fungus H5; T2, fungus H20; T3, bacteria B3; T4,control. The variables evaluated on the cocoa fruits were incidenceand external and internal severity. The results of the externaland internal severity showed that with respect to the control thedamage reduction on the cocoa fruits was 19,5 and 11,2% in T1,28 and 19,5% in T2, and 13,5 and 8,5% in T3, respectively withstatistical differences in favor of T2. It is concluded that amongthe three evaluated antagonists, the fungus H20 (Trichoderma sp.has the greatest potential for the biocontrol of frosty pod rot in thecocoa cultivation under field conditions.

  18. Leptin, IL-6, and suPAR reflect distinct inflammatory changes associated with adiposity, lipodystrophy and low muscle mass in HIV-infected patients and controls

    DEFF Research Database (Denmark)

    Langkilde, Anne; Petersen, Janne; Henriksen, Jens Henrik

    2015-01-01

    was significantly positively associated with fat mass index (FMI) and abdominal VAT, but not with lean mass index (LMI). IL-6 was significantly associated with both FMI and VAT, and low LMI. High suPAR was associated with low LMI, and weakly with high FMI and VAT. CONCLUSIONS: Leptin reflected adiposity...

  19. The receptor for urokinase-plasminogen activator (uPAR) controls plasticity of cancer cell movement in mesenchymal and amoeboid migration style

    National Research Council Canada - National Science Library

    Margheri, Francesca; Luciani, Cristina; Taddei, Maria Letizia; Giannoni, Elisa; Laurenzana, Anna; Biagioni, Alessio; Chillà, Anastasia; Chiarugi, Paola; Fibbi, Gabriella; Del Rosso, Mario

    2014-01-01

    .... These features are typical of mesenchymal motility. Here we show that the full-length form of uPAR is required when prostate and melanoma cancer cells convert their migration style from the "path generating" mesenchymal to the "path finding" amoeboid...

  20. Croissance epitaxiale de GaAs sur substrats de Ge par epitaxie par faisceaux chimiques

    Science.gov (United States)

    Belanger, Simon

    de confirmer l'efficacite du procede de preparation de surface et d'identifier les conditions de croissance optimales. Les resultats de caracterisation indiquent que les materiaux obtenus presentent une tres faible rugosite de surface, une bonne qualite cristalline et un dopage residuel relativement important. De plus, l'interface GaAs/Ge possede une faible densite de defauts. Finalement, la diffusion d'arsenic dans le substrat de germanium est comparable aux valeurs trouvees dans la litterature pour la croissance a basse temperature avec les autres procedes d'epitaxie courants. Ces resultats confirment que la technique d'epitaxie par faisceaux chimiques (CBE) permet de produire des couches de GaAs sur Ge de qualite adequate pour la fabrication de cellules solaires a haute performance. L'apport a la communaute scientifique a ete maximise par le biais de la redaction d'un article soumis a la revue Journal of Crystal Growth et la presentation des travaux a la conference Photovoltaics Canada 2010 . Mots-cles : Epitaxie par jets chimiques, Chemical beam epitaxy, CBE, MOMBE, Germanium, GaAs, Ge

  1. Should we routinely remove the dose delivered by the images of control?; Faut-il systematiquement retirer la dose delivree par les images de controle?

    Energy Technology Data Exchange (ETDEWEB)

    Goasduff, G. [Centre Hospitalier Universitaire Morvan, Service de Radiotherapie, 29 - Brest (France); Pene Baverez, D.; Pradier, O.; Bouchekoua, M. [Service de Radiotherapie, 29 - Brest (France)

    2009-10-15

    The constraints of doses fixed by the international commission on radiation units and measurements (ICRU) for the target volume (95-107% of the prescribed dose) are respected. for the hypo fractionated treatments, it is necessary to control the impact of the dose delivered by the control images for every patient. The dose delivered at the isocenter is estimated between 1 and 3 Gy by control image: this dose depends on the beams size and on the distance-source-skin. Protocols of the patient positioning checking must be implemented on optimizing their frequency to limit the dose received by the patient. (N.C.)

  2. Analytical methods for describing charged particle dynamics in general focusing lattices using generalized Courant-Snyder theory

    CERN Document Server

    Qin, Hong; Burby, J W; Chung, Moses

    2015-01-01

    The dynamics of charged particles in general linear focusing lattices with quadrupole, skew-quadrupole, dipole, and solenoidal components, as well as torsion of the fiducial orbit and variation of beam energy is parameterized using a generalized Courant-Snyder (CS) theory, which extends the original CS theory for one degree of freedom to higher dimensions. The envelope function is generalized into an envelope matrix, and the phase advance is generalized into a 4D symplectic rotation, or an U(2) element. The 1D envelope equation, also known as the Ermakov-Milne-Pinney equation in quantum mechanics, is generalized to an envelope matrix equation in higher dimensions. Other components of the original CS theory, such as the transfer matrix, Twiss functions, and CS invariant (also known as the Lewis invariant) all have their counterparts, with remarkably similar expressions, in the generalized theory. The gauge group structure of the generalized theory is analyzed. By fixing the gauge freedom with a desired symmetr...

  3. PAR Loop Schedule Review

    Energy Technology Data Exchange (ETDEWEB)

    Schaffer, Jr.; W.F.

    1958-04-30

    The schedule for the installation of the PAR slurry loop experiment in the South Facility of the ORR has been reviewed and revised. The design, fabrications and Installation is approximately two weeks behind schedule at this time due to many factors; however, indications are that this time can be made up. Design is estimated to be 75% complete, fabrication 32% complete and installation 12% complete.

  4. La pelade par plaques

    Science.gov (United States)

    Spano, Frank; Donovan, Jeff C.

    2015-01-01

    Résumé Objectif Présenter aux médecins de famille des renseignements de base pour faire comprendre les schémas thérapeutiques et les résultats des traitements pour la pelade par plaques, de même que les aider à identifier les patients pour qui une demande de consultation en dermatologie pourrait s’imposer. Sources des données Une recension a été effectuée dans PubMed pour trouver des articles pertinents concernant le traitement de la pelade par plaques. Message principal La pelade par plaques est une forme auto-immune de perte pileuse qui touche à la fois les enfants et les adultes. Même s’il n’y a pas de mortalité associée à la maladie, la morbidité découlant des effets psychologiques de la perte des cheveux peut être dévastatrice. Lorsque la pelade par plaques et le sous-type de la maladie sont identifiés, un schéma thérapeutique approprié peut être amorcé pour aider à arrêter la chute des cheveux et possiblement faire commencer la repousse. Les traitements de première intention sont la triamcinolone intralésionnelle avec des corticostéroïdes topiques ou du minoxidil ou les 2. Les médecins de famille peuvent prescrire ces traitements en toute sécurité et amorcer ces thérapies. Les cas plus avancés ou réfractaires pourraient avoir besoin de diphénylcyclopropénone topique ou d’anthraline topique. On peut traiter la perte de cils avec des analogues de la prostaglandine. Les personnes ayant subi une perte de cheveux abondante peuvent recourir à des options de camouflage ou à des prothèses capillaires. Il est important de surveiller les troubles psychiatriques en raison des effets psychologiques profonds de la perte de cheveux. Conclusion Les médecins de famille verront de nombreux patients qui perdent leurs cheveux. La reconnaissance de la pelade par plaques et la compréhension du processus pathologique sous-jacent permettent d’amorcer un schéma thérapeutique approprié. Les cas plus graves ou r

  5. Ultrasound control modelization at the Atomic Energy Commission; La modelisation du controle par ultrasons au commissariat a l`energie atomique

    Energy Technology Data Exchange (ETDEWEB)

    Calmon, P.; Lecoeur-Taibi, I.; Lhemery, A. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Dept. des Procedes et Systemes Avances

    1995-10-01

    The Champ-Sons code (for the ultrasonic field modelization) and of the Mephisto code (prediction of the ultrasonic echography from the a priori knowledge of the controlled piece and of the encountered defects) are presented with three applications: pipes with inner or outer cracks, rocket tank fuel with a welded joint and PWR pressure vessel with an irregular coating. (A.B.). 7 refs., 4 figs.

  6. Control and diagnosis oriented modelling of the compression ignition engine; Modelisation du moteur a allumage par compression dans la perspective du controle et du diagnostic

    Energy Technology Data Exchange (ETDEWEB)

    Grondin, O.

    2004-12-15

    This thesis has described an investigation into the modelling of compression ignition engine for control and diagnosis purpose. The Diesel engine is the most efficient and clean internal combustion engine due to modem electromechanical actuators. However, pollutant emission regulations are much more stricter, thus, these complex systems need sophisticated and efficient control algorithms to reach very low emission levels. For this task, engine models are required at each step of the control system development: control laws synthesis, simulation and validation. The system under study is a six cylinder direct injection Diesel engine fitted with a turbocharger. The model of this system is based on physical laws for some parts of the engine such as cylinders, manifolds, turbocharger and crank-slider system. In order to reduce computing time we choose to model heat transfer and heat release during combustion using simple empirical correlations. Resulting model has been implemented in the Matlab-Simulink environment and it can predict variables of interest for control purpose with one degree crank angle resolution. The model has been tested numerically and compared with an industrial engine simulation code with good results. Moreover, model output variables are in good agreement with experimental data recorded on a heavy-duty research engine. The engine model has been embedded on a board providing enough computing performances to perform real-time simulations, this will be helpful for 'hardware-in-the-loop' simulations. Another part of this study is dedicated to the combustion process modelling using a non linear phenomenological model: the NARMAX model. The goal is to predict the in-cylinder pressure evolution using other measurements available on the engine. The NARMAX model parameters have been identified using input-output data carried out from the experimental engine. Such model is well suited for real-time applications compare to numerically cost

  7. Development of a model of the interaction of an electromagnetic field with an ideal planar defect within a conductive medium. Application to eddy current non destructive testing; Developpement d'un modele d'interaction d'un champ electromagnetique avec un defaut plan dans un materiau conducteur. Application a la simulation d'un procede de controle non destructif par courants de Foucault

    Energy Technology Data Exchange (ETDEWEB)

    Maurice, L

    2006-12-15

    The Eddy Current (EC) Non Destructive Testing (NDT) is used in various industrial fields. The modelling of the implied phenomena brings a substantial hand for the understanding, the set-up and the optimisation of the investigations to be performed. It requires the resolution of the Maxwell equations that can be achieved with semi-analytical models, based on an integral methods. The Volume Integral Method (VIM) enables to simulate various EC NDT configurations involving volumetric defects. Nevertheless, important formalism and numerical problems appear when the considered defect has a small aperture, and computational times become very high. This is the reason why the Surface Integral Model (SIM) has been developed. The defect is taken into account as a source layer of current dipole. Based on this assumption, the existence of a scalar potential quantity can be demonstrated. A study of different numerical implementations enables to select the most efficient. Then, validations have been carried out, comparing probe responses, computed by SIM, VIM, to experimental data. The SIM model turns out to be very fast, and well adapted to get 2-D probe responses, with good accuracy. Nevertheless, the hypotheses used in the SIM formalism appeared to be very restrictive for some configurations. A model has been set up, that takes advantages of both VIM and SIM. The dipole density evaluation is still based on surface hypotheses, therefore the computational load is comparable to the ones of SIM. The computations are then improved by the evaluation of the total field, which enables to partially take into account the defect aperture. Another set of comparisons between data obtained by the three simulation models and experimental measurements has been carried out, for numerous configurations. Some of them namely imply probes made of two coils, separately transmitting and receiving.The new 'hybrid' model brings suitable corrections to the SIM model. The simulation results become very satisfactory for all tested NDT configurations, with an accuracy of the order of those brought by the VIM model as well as computation load and time comparable to those of the SIM. This hybrid model will be integrated in fine to CNA. (author)

  8. A fly-wheel drive with controlled-torque clutch for a reactors cooling circuit pumps; Entrainement des pompes du circuit de refrigeration d'un reacteur par volant a embrayage sous couple controle

    Energy Technology Data Exchange (ETDEWEB)

    Riettini, A. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1964-10-15

    After a theoretical study on the slowing down of a centrifugal pump, the motion equations have been checked by means of experimental tests. In order to have important slowing down times (which is the case of the cooling pumps of a research reactor) it is necessary to add a fly-wheel. To prevent troubles when starting, a block pump-fly-wheel with clutch under controlled torque was developed. It is so possible to start the fly-wheel progressively without increasing too much power of the driving motor. (author) [French] Apres une etude theorique sur le mouvement de ralentissement d'une pompe centrifuge, les equations du mouvement ont ete verifiees par des essais pratiques. Pour obtenir des temps de ralentissement importants (cas des pompes de refrigeration d'un reacteur de recherche) il est necessaire d'y adjoindre un volant d'inertie. Pour eviter les inconvenients au demarrage, on a etudie un ensemble pompe-volant avec embrayage sous couple controle. Cette solution permet de lancer progressivement le volant sans augmentation appreciable de la puissance du moteur d'entrainement. (auteur)

  9. Parálisis cerebral :

    OpenAIRE

    Giral Lamenca, Mónica

    2015-01-01

    Se aborda el tema de la parálisis cerebral definiendo qué es, clasificando los tipos de parálisis dependiendo de la afectación y las características principales. Se explican algunos de sus tratamientos, se dan sistemas alternativos y/o aumentativos de comunicación para un alumno con PC (parálisis cerebral).

  10. Câbles électriques : Calcul du courant admissible : Partie 3: Sections concernant les conditions de fonctionnement : Section 2: Optimisation économique des sections d'âme de câbles électriques de puissance

    CERN Document Server

    International Electrotechnical Commission. Geneva

    1995-01-01

    Câbles électriques : Calcul du courant admissible : Partie 3: Sections concernant les conditions de fonctionnement : Section 2: Optimisation économique des sections d'âme de câbles électriques de puissance

  11. Activités biologiques du Seigle et du Sorgho chez le rat « Wistar » rendu diabétique par la Streptozotocine

    OpenAIRE

    MAMI, épouse SOUALEM Zoubida

    2015-01-01

    Le changement des habitudes alimentaires au cours de ces dernières années a abouti à l’apparition des maladies dites de civilisations, entre autre le diabète sucré caractérisé par une hyperglycémie courante causée par un déficit de sécrétion de l’insuline ou résistance cellulaire à l’insuline. Des approches diéto-thérapeutiques privilégient la consommation des produits céréaliers sous la forme la plus complète possible. Le Sorgho (Sorghum bicolor L.), espèce très connue comme source important...

  12. Á contre-courant : les mobilités caribéennes vers l’Éthiopie

    Directory of Open Access Journals (Sweden)

    Giulia Bonacci

    2008-09-01

    Full Text Available L’article s’intéresse à la migration des Caribéens vers l’Ethiopie, et notamment celle des Rastafariens qui se définissent comme de « vrais Éthiopiens » et se disent « rapatriés » en Éthiopie. En dépit de leur nombre limité, ils n’en sont pas moins importants car ils illustrent la persistance et les transformations des imaginaires et idéologies liant les Noirs du monde à l’Éthiopie ainsi qu’un pan méconnu des mobilités caribéennes qui, se dirigeant vers l’Afrique, se découvrent complètement à contre-courant.The article deals with Caribbean migration towards Ethiopia, and especially the one of Rastafarians who define themselves as ‘true Ethiopians’ and ‘repatriates’ in Ethiopia. Despite their limited number, their experience is nonetheless important as it reveals the persistence and change in imaginaries and ideologies linking black all over the world to Ethiopia as well as unknown Caribbean mobility towards Africa, a mobility which is driven against the current.

  13. Câbles électriques - Calcul du courant admissible - Partie 2: Résistance thermique - Section 2: Méthode de calcul des coefficients de réduction de l'intensité de courant admissible pour des groupes de câbles posés à l'air libre et protégés du rayonnement solaire direct

    CERN Document Server

    1995-01-01

    Câbles électriques - Calcul du courant admissible - Partie 2: Résistance thermique - Section 2: Méthode de calcul des coefficients de réduction de l'intensité de courant admissible pour des groupes de câbles posés à l'air libre et protégés du rayonnement solaire direct

  14. ParB spreading requires DNA bridging

    NARCIS (Netherlands)

    Graham, Thomas G. W.; Wang, Xindan; Song, Dan; Etson, Candice M.; van Oijen, Antoine M.; Rudner, David Z.; Loparo, Joseph J.

    2014-01-01

    The parABS system is a widely employed mechanism for plasmid partitioning and chromosome segregation in bacteria. ParB binds to parS sites on plasmids and chromosomes and associates with broad regions of adjacent DNA, a phenomenon known as spreading. Although essential for ParB function, the mechani

  15. Correlation between symptoms and sagittal alignment parameters in patients with lumbar canal stenosis: a case-control study Correlación entre síntomas y parámetros de alineación sagital en pacientes con estenosis del canal lumbar: un estudio de control de casos Correlação entre os sintomas e os parâmetros de alinhamento sagital em pacientes com estenose do canal lombar: estudo de caso-controle

    Directory of Open Access Journals (Sweden)

    Paulo Tadeu Maia Cavali

    2012-12-01

    Full Text Available OBJECTIVE: To examine the relationship between sagittal balance parameters and different symptoms of spinal disease in patients with lumbar canal stenosis (LCS and controls. METHODS: In this prospective, diagnostic, case-control study, we included all patients consecutively admitted to a public teaching hospital for surgical treatment of LCS between July 2010 and October 2011, aged more than 40 years, with back pain plus radiculopathy or neurogenic claudication, and controls without LCS. Magnetic resonance and x-rays allowed the measurement of sagittal axis parameters. Clinical data, the Oswestry Disability Index and the visual analogue scale of pain were assessed. RESULTS: 23 patients were in the Stenosis group, and 17 were controls. The Stenosis group presented lower values of total lumbopelvic lordosis and regional lordosis L1, L2 and L3. In LCS patients and back pain, total lumbopelvic and regional lordosis at L1, L2 and L3 were smaller. Those with stenosis and radiculopathy had higher values of pelvic tilt and lower total lumbopelvic lordosis and regional lordosis in L1 and L2. In patients with claudication, regional lumbopelvic lordosis in L1 and L2 and the T9 sagittal offset were smaller. All patients with pain had higher values of thoracic kyphosis, regional lumbopelvic lordosis in L1, lower values for pelvic tilt, sagittal T1 offset, sacro-femoral distance and overhang compared to patients without pain. CONCLUSIONS: This study shows significant correlations between symptoms and sagittal axis parameters between patients with and without spinal canal stenosis and also in subgroups of the patients with stenosis with different complaints.OBJETIVO: Analizar la relación entre los parámetros del equilibrio sagital y los diversos síntomas de enfermedad espinal en pacientes con estenosis del canal lumbar (ECL y sus controles. MÉTODOS: En esta perspectiva, de estudio de diagnóstico, de control de casos, incluimos a todos los pacientes

  16. Metodología para la implementación del diseño económico y/o estadístico de cartas de control x-barra con parámetros variables (VP

    Directory of Open Access Journals (Sweden)

    Rita Peñabaena-Niebles

    2014-01-01

    Full Text Available Los últimos avances en el diseño de cartas de control se han enfocado en las cartas con parámetros variables (VP en las cuales se modifica los parámetros de la carta de acuerdo al valor de la última muestra. Estas cartas han demostrado ser más eficaces en la detección de las causas asignables pero más complejas para su implementación en las organizaciones. Aunque es mucha la literatura referente al diseño económico y/o estadístico de cartas VP, son pocos los autores que enfocan sus esfuerzos en hacerlas accesibles a las empresas. En este artículo se presentara un nuevo procedimiento diseñado con el fin de disminuir la complejidad y aprovechamiento de las ventajas del diseño económico y económico-estadístico de cartas X con VP.

  17. Carrier currents systems and home integrated systems; Les courants porteurs vont-ils epanouir la domotique

    Energy Technology Data Exchange (ETDEWEB)

    Remond, C.

    1996-12-31

    Energy savings in buildings can be performed by remote control applications. Current carrier equipment interfaces use buildings integrated power network to perform data transmission through an entire building, or even to a remote building. The case of industrial buildings lighting systems is sketched out, but these interfaces apply as well to electric heating (space or water heating). (D.L.)

  18. Nanopositionnement 3D à base de mesure à courant tunnel et piezo-actionnement

    OpenAIRE

    Ryba, Lukasz

    2015-01-01

    The objective of this thesis was to elaborate high performance control strategies and their real-time validation on a tunneling current-based 3D nanopositioning system developed in GIPSA-lab. The thesis lies in the domain of micro-/nano mechatronic systems (MEMS) focused on applications of fast and precise positioning and scanning tunneling microscopy (STM). More precisely, the aim is to position the metallic tunneling tip (like in STM) over the metallic surface using piezoelectric actuators ...

  19. Analytical methods for describing charged particle dynamics in general focusing lattices using generalized Courant-Snyder theory

    Directory of Open Access Journals (Sweden)

    Hong Qin

    2014-04-01

    Full Text Available The dynamics of charged particles in general linear focusing lattices with quadrupole, skew-quadrupole, dipole, and solenoidal components, as well as torsion of the fiducial orbit and variation of beam energy is parametrized using a generalized Courant-Snyder (CS theory, which extends the original CS theory for one degree of freedom to higher dimensions. The envelope function is generalized into an envelope matrix, and the phase advance is generalized into a 4D symplectic rotation, or a U(2 element. The 1D envelope equation, also known as the Ermakov-Milne-Pinney equation in quantum mechanics, is generalized to an envelope matrix equation in higher dimensions. Other components of the original CS theory, such as the transfer matrix, Twiss functions, and CS invariant (also known as the Lewis invariant all have their counterparts, with remarkably similar expressions, in the generalized theory. The gauge group structure of the generalized theory is analyzed. By fixing the gauge freedom with a desired symmetry, the generalized CS parametrization assumes the form of the modified Iwasawa decomposition, whose importance in phase space optics and phase space quantum mechanics has been recently realized. This gauge fixing also symmetrizes the generalized envelope equation and expresses the theory using only the generalized Twiss function β. The generalized phase advance completely determines the spectral and structural stability properties of a general focusing lattice. For structural stability, the generalized CS theory enables application of the Krein-Moser theory to greatly simplify the stability analysis. The generalized CS theory provides an effective tool to study coupled dynamics and to discover more optimized lattice designs in the larger parameter space of general focusing lattices.

  20. Theory and Practice of a New Class of Equipment for Separation of Particulates from Gases: the Turbulent Flow Precipitator Le précipitateur turbulent : un nouveau type de dispositif de séparation de particules présentes dans un courant de gaz. Théorie et technologie

    Directory of Open Access Journals (Sweden)

    Dullien F. A. L.

    2006-12-01

    Full Text Available The main classes of separators of particulates from gases comprise inertial separators, filters, (wet scrubbers and electrostatic precipitators, each of which technique has its proper niche and each has its advantages and its disadvantages. In the present paper a new class of equipment, the turbulent flow precipitator (TFP for gas cleaning is described that, though at the present only in its infancy, is rapidly gaining popularity because it has some advantages over each and every known type of separator and it has very few limitations. TFPs are far more efficient for the removal of fine particles than inertial separators, unlike filters they do not plug up, they do not present secondary disposal problems and they require less maintenance (and are less expensive than electrostatic precipitators. They can be very efficient also in the submicron range of particle size and there are no practical limitations as far as high temperatures and/or corrosive atmospheres are concerned. They are equally suitable for the removal of solid and liquid particles from gases. Turbulent flow precipitators work on the principle of turbulent eddy penetration into deep regions where there is no net flow, where the eddies die out and where the fine particulates carried by the eddies deposit on collector surfaces. TFPs comprise two well defined regions:- straight, uniform and unobstructed flow passages in which the gas carrying the suspended fine particulates is passed in turbulent flow;- and adjacent collection regions where there is no net gas flow and where all of the separation of particles from the gas takes place. Hence, a TFP is a filter in which the separation of the particulates from the gas takes place outside the passages where the gas flows. Les principaux séparateurs de particules dans un courant de gaz existants sur le marché sont les séparateurs par inertie, les filtres, les laveurs et les électrofiltres. Chaque type de séparateur est destiné à une

  1. Elusloom lennukiga puhkusele / Inge Parring

    Index Scriptorium Estoniae

    Parring, Inge

    2003-01-01

    Ilmunud ka: Delovõje Vedomosti 1. okt. lk. 13. Air Cargo Estonia/ACE Logisticsi müügijuht Inge Parring tutvustab elusloomade transpordivõimalusi. Vt. samas: Loomade transportimiseks vajalikud dokumendid

  2. Pars planitis in a family.

    Science.gov (United States)

    Tejada, P; Sanz, A; Criado, D

    1994-01-01

    The familial occurrence of pars planitis is rare. We have found ten cases reported previously. We describe a new case of pars planitis in a family. The affected members included a mother and two of her four children. The family was tested for HLA antigens in order to establish a comparison with others HLA types by different authors. We have not identified any cause for the familial occurrence of this disease. We discuss the role of genetic and ambiental factors.

  3. Prokaryotic ParA-ParB-parS system links bacterial chromosome segregation with the cell cycle.

    Science.gov (United States)

    Mierzejewska, Jolanta; Jagura-Burdzy, Grażyna

    2012-01-01

    While the essential role of episomal par loci in plasmid DNA partitioning has long been appreciated, the function of chromosomally encoded par loci is less clear. The chromosomal parA-parB genes are conserved throughout the bacterial kingdom and encode proteins homologous to those of the plasmidic Type I active partitioning systems. The third conserved element, the centromere-like sequence called parS, occurs in several copies in the chromosome. Recent studies show that the ParA-ParB-parS system is a key player of a mitosis-like process ensuring proper intracellular localization of certain chromosomal regions such as oriC domain and their active and directed segregation. Moreover, the chromosomal par systems link chromosome segregation with initiation of DNA replication and the cell cycle.

  4. Control of critical parameters for square cup deep drawing of AISI 304 DDQ using genetic algorithm//Control de parámetros críticos en la embutición de una copa rectangular de AISI 304 DDQ empleando algoritmo genético

    Directory of Open Access Journals (Sweden)

    Yudieski Bernal-Aguilar

    2013-05-01

    Full Text Available AbstractsThe main purpose of this work is to develop an intellectualized control technique on the deep drawing of square cup made of AISI 304 DDQ stainless steel using genetic algorithm. These control methods are employed in order to investigate the most significant parameters in sheet metal forming process such as drawing force, with a view of optimizing these parameters. The genetic algorithm is used for theoptimization purpose to minimize the force of the deep drawing process and to investigate the roles of other parameters as blank holder force. Experimental results show that these combinations of control system can cover a wide range of both materials and influential forming parameters automatically. The results further confirm that the developed system is effective and valid alternative for quick responsible control system with high flexibility.Key words: intelligent control, deep drawing, genetic algorithm, sheet metal forming._______________________________________________________________________________ResumenEl objetivo principal del trabajo es desarrollar un método  de control inteligente para la embutición de una pieza rectangular fabricada en AISI DDQ empleando algoritmo genético. Este método de control se utiliza para investigar parámetros críticos en el proceso de conformado de chapa metálica, tales como la fuerza en el prensachapas y  la fuerza máxima de embutición, con el fin de minimizar este parámetro. Los resultados experimentales obtenidos demuestran que el método de control puede cubrir un amplio espectro de combinaciones, tanto para los materiales, como para los parámetros críticos y es una técnica alternativa y flexible, válida para desarrollar sistemas de control en los procesos de embutición con gran flexibilidad. Palabras claves: control inteligente, embutición profunda, algoritmo genético, conformado de chapa metálica

  5. A relation between the multiplicity of the second eigenvalue of a graph Laplacian, Courant's nodal line theorem and the substantial dimension of tight polyhedral surfaces

    CERN Document Server

    Tlusty, Tsvi

    2010-01-01

    This note discusses a relation between the multiplicity m of the second eigenvalue {\\lambda}2 of a Laplacian on a graph G, tight mappings of G and a discrete analogue of Courant's nodal line theorem. For a certain class of graphs, we show that the m-dimensional eigenspace of {\\lambda}2 is tight and thus defines a tight mapping of G into an m-dimensional Euclidean space. The tightness of the mapping is shown to set Colin de Verdi\\`ere's upper bound on the maximal {\\lambda}2-multiplicity, where chr({\\gamma}(G)) is the chromatic number and {\\gamma}(G) is the genus of G.

  6. suPAR: the molecular crystal ball

    DEFF Research Database (Denmark)

    Thunø, Maria; Macho, Betina; Eugen-Olsen, Jesper

    2009-01-01

    soluble urokinase Plasminogen Activator Receptor (suPAR) levels reflect inflammation and elevated suPAR levels are found in several infectious diseases and cancer. suPAR exists in three forms; suPAR(I-III), suPAR(II-III) and suPAR(I) which show different properties due to structural differences....... Studies suggest that full-length suPAR is a regulator of uPAR/uPA by actingas uPA-scavenger, whereas the cleaved suPAR(II-III) act as a chemotactic agent promoting the immune response via the SRSRY sequence in the linker-region. This review focus on the various suPAR fragments and their involvement...

  7. The ParB-parS Chromosome Segregation System Modulates Competence Development in Streptococcus pneumoniae

    NARCIS (Netherlands)

    Attaiech, Laetitia; Minnen, Anita; Kjos, Morten; Gruber, Stephan; Veening, Jan-Willem

    2015-01-01

    UNLABELLED: ParB proteins bind centromere-like DNA sequences called parS sites and are involved in plasmid and chromosome segregation in bacteria. We previously showed that the opportunistic human pathogen Streptococcus pneumoniae contains four parS sequences located close to the origin of replicati

  8. Optic capture pars plana lensectomy

    Directory of Open Access Journals (Sweden)

    Lee JE

    2012-10-01

    Full Text Available Joo Eun LeeDepartment of Ophthalmology, Inje University College of Medicine, Busan, South KoreaObjective: To describe an optic capture pars plana lensectomy technique.Methods: After core vitrectomy, pars plana lensectomy is performed with preservation of the anterior capsule. Capsulorhexis is performed on the preserved anterior capsule through a 2.8 mm clear corneal incision. An intraocular lens (IOL is placed in the ciliary sulcus, and then the optic of the IOL is pushed back to the vitreous cavity so that the optic is captured by the surrounding capsulorhexis margin.Results: The captured IOL-capsule diaphragm remained stable during air–fluid exchange and prevented air prolapse to the anterior chamber. IOL stability and a clear visual axis were preserved during the follow-up period.Conclusion: With this modified pars plana lensectomy technique, stable IOL position and clear visual axis can be maintained when a pars plana approach is needed during combined cataract and vitreoretinal surgery.Keywords: lensectomy, optic capture, pars plana lensectomy, vitrectomy

  9. Designing Tone Reservation PAR Reduction

    Directory of Open Access Journals (Sweden)

    Johansson Albin

    2006-01-01

    Full Text Available Tone reservation peak-to-average (PAR ratio reduction is an established area when it comes to bringing down signal peaks in multicarrier (DMT or OFDM systems. When designing such a system, some questions often arise about PAR reduction. Is it worth the effort? How much can it give? How much does it give depending on the parameter choices? With this paper, we attempt to answer these questions without resolving to extensive simulations for every system and every parameter choice. From a specification of the allowed spectrum, for instance prescribed by a standard, including a PSD-mask and a number of tones, we analytically predict achievable PAR levels, and thus implicitly suggest parameter choices. We use the ADSL2 and ADSL2+ systems as design examples.

  10. Caractéristiques dynamiques d'un écoulement co-courant eau-huile-air Dynamic Characteristics of a Co-Current Water-Oil-Air Flow

    Directory of Open Access Journals (Sweden)

    Fabre J.

    2006-11-01

    Full Text Available Les auteurs présentent une étude expérimentale sur la structure locale d'un écoulement quasi parallèle co-courant à trois phases : eau-huile-air. L'objectif principal de cette étude est la détermination des contraintes de cisaillement aux interfaces eau-huile et air-huile. L'écoulement est réalisé dans un canal rectangulaire équipé d'une soufflerie et d'un système pour séparer et recycler les phases liquides, en continu. Les résultats expérimentaux présentés sont relatifs aux régimes lisses des interfaces : les vitesses et leurs fluctuations sont mesurées par anémométrie laser dans l'eau et anémométrie à film chaud dans l'air. Les résultats sur l'interface gaz-liquide confirment que l'écoulement est identique à celui sur une paroi se déplaçant à la vitesse du liquide à la surface. Dans l'eau, la structure est celle d'un écoulement de Couette turbulent. Près de la paroi inférieure et près de l'interface liquide-liquide les échelles locales de vitesse et de longueur regroupent assez bien les mesures de vitesses et de fluctuations turbulentes longitudinales. This article is an experimental investigation, of the local structure of a quasi-parallel co-current three-phase flow invoiving water, oil and air. The main aim of this investigation is to determine the shear stresses on the water-oil and air-oil interfaces. Flow is induced in a rectangular channel equipped with a wind tunnel and a system for separating and recirculating the liquid phases continuously. The experimental results that are given have to do with smooth interface regimes. Velocities and their fluctuations are measured by laser anemometry in water and by hot-wire anenometry in air. The results on the gas-liquid interface confirm that flow is identical to flow over a wall moving at the velocity of the liquid on the surface. In water, the structure is that of a turbulent Couette flow. Near the bottom wall and near the liquid-liquid interface, the

  11. Vision par ordinateur: outils fondamentaux

    OpenAIRE

    Horaud, Radu; Monga, Olivier

    1995-01-01

    National audience; Cet ouvrage présente les outils fondamentaux de la vision par ordinateur dans un langage mathématique accessible aux étudiants de niveau DEUG en mathématiques ou informatique. Il donne également de nombreux exemples d'utilisation de la vision par ordinateur dans deux domaines de technologie de pointe : la robotique et l'imagerie médicale. Cette deuxième édition largement augmentée est un manuel de référence. Les outils fondamentaux sont présentés dans un langage mathématiqu...

  12. Etude experimentale et optimisation d'un systeme hybride hydraulique pour camions a ordures et amelioration des performances par raffinement de sa logique de controle

    Science.gov (United States)

    Lacroix, Benoit

    The aim of this thesis is to demonstrate experimentally the operation of a hydraulic hybrid system specifically dedicated to the application of refuse trucks in addition to proposing solutions to improve its control strategy. The developed hybrid system recovers the vehicle's kinetic energy during braking. A variable displacement hydraulic motor then uses the energy stored in a hydraulic accumulator to assist the internal combustion engine (ICE) at suitable times. The particular aspect of this system is that assistance to the ICE can occur when it operates at idle and drives the auxiliary hydraulic equipment of the refuse truck. Essentially, the control strategy initially developed maximizes the recovery of braking energy and uses that energy to minimize the solicitation of the ICE at idle. The experimental results obtained with two prototypes tested in real operating conditions show that the hybrid system can recover a significant portion of braking energy. In addition, the results show that it is possible to reduce the load on the ICE during idle with the application of an assisting torque. However, the advantage of assisting the ICE in specific areas of the operating range is slim since the ICE's gross efficiency varies only slightly depending on conditions of operation. This is confirmed by the optimization of the control logic using deterministic dynamic programming. Indeed, by managing the pressure in the accumulator to maximize the amount of energy recovered during braking and by dosing the assistance to the ICE in an ideal fashion, the optimal control only managed to improve fuel savings by 6% in comparison to the original control. Therefore, since the efforts that would be required to emulate the ideal behavior in real time are significant for a relatively small and uncertain gain, the initial control logic is considered near optimal. Finally, this thesis proposes an improved version of the torque assisting hybrid system that could shut down the ICE when

  13. Controle sismique d'un batiment en acier de 3 etages a l'echelle 1/3 par amortisseurs elastomeres et contreventements en chevron

    Science.gov (United States)

    Gauron, Olivier

    This study develops an innovative configuration of seismic natural rubber dampers for multistory low- and medium-rise steel braced frames. The dampers are directly integrated in an horizontal position in the seismic force resisting system of the structure. They are connected in a series with typical chevron brace systems. This control system provides not only additional structural damping to the structure but also a period shift, acting in the same way as a base isolation system. First, the fiber reinforced natural rubber used in the application was tested. It exhibited strong non linear dependance of its equivalent viscoelastic properties related to the shear strain. Then, a 1/3-scale 3-story chevron braced steel frame with and without dampers was considered. The structure was build and placed on the shaking table of the University of Sherbrooke Structures Laboratory. Numerical studies show that the efficiency of the control system reduces strongly the seismic induced forces of the undamped structure without any amplification of displacement or drift. Obtained seismic response reduction levels represent significant safety and economical benefits for the proposed application. Finally, the control system viability is experimentally demonstrated by shaking table tests at different reduced seismic intensities. Non linear behavior of the structure due to non linear behavior of the damping material is highlighted, and the dependance of seismic control performances is shown to be related to seismic intensities. Results allow an extrapolation of the experimental control peformances tending to the numerical results at higher intensities. Key-words : chevron braces, damper, multistory building, natural rubber, seismic control, shaking table.

  14. A model for the condensation of the bacterial chromosome by the partitioning protein ParB

    Science.gov (United States)

    Broedersz, Chase; Wingreen, Ned

    2013-03-01

    The molecular machinery responsible for faithful segregation of the chromosome in bacteria such as Caulobacter crescentus and Bacillus subtilis includes the ParABS a.k.a. Spo0J/Soj partitioning system. In Caulobacter, prior to division, hundreds of ParB proteins bind to the DNA near the origin of replication, and localize to one pole of the cell. Subsequently, the ParB-DNA complex is translocated to the far pole by the binding and retraction of the ParA spindle-like apparatus. Remarkably, the localization of ParB proteins to specific regions of the chromosome appears to be controlled by only a few centromeric parS binding sites. Although lateral interactions between DNA-bound ParB are likely to be important for their localization, the long-range order of ParB domains on the chromosome appears to be inconsistent with a picture in which protein-protein interactions are limited to neighboring DNA-bound proteins. We developed a coarse-grained Brownian dynamics model that allows for lateral and 3D protein-protein interactions among bound ParB proteins. Our model shows how such interactions can condense and organize the DNA spatially, and can control the localization and the long-range order of the DNA-bound proteins.

  15. ParSplice, Version 1

    Energy Technology Data Exchange (ETDEWEB)

    2017-01-05

    The ParSplice code implements the Parallel Trajectory Splicing algorithm described in [1]. This method is part of the Accelerated Molecular Dynamics family of techniques developed in Los Alamos National Laboratory over the last 16 years. These methods aim at generating high-quality trajectories of ensembles of atoms in materials. ParSplice uses multiple independent replicas of the system in order to parallelize the generation of such trajectories in the time domain, enabling simulations of systems of modest size over very long timescales. ParSplice includes capabilities to store configurations of the system, to generate and distribute tasks across a large number of processors, and to harvest the results of these tasks to generate long trajectories. ParSplice is a management layer that orchestrate large number of calculations, but it does not perform the actual molecular dynamics itself; this is done by external molecular dynamics engines. [1] Danny Perez, Ekin D Cubuk, Amos Waterland, Efthimios Kaxiras, Arthur F Voter, Long-time dynamics through parallel trajectory splicing, Journal of chemical theory and computation 12, 18 (2015)

  16. Parâmetros genéticos para produção de leite no dia do controle de vacas da raça Holandesa utilizando modelos de análises de fatores e componentes principais

    Directory of Open Access Journals (Sweden)

    Mariana de Almeida Dornelles

    2015-06-01

    Full Text Available Objetivou-se comparar um modelo multi-característica padrão com modelos de análise de fatores (AF e de componentes principais (CP para estimar parâmetros genéticos para a produção de leite no dia do controle (PLDC de vacas da raça Holandesa. O arquivo de trabalho constituiu-se de 4.616 registros mensais de PLDC de primeiras lactações de vacas da raça Holandesa. As PLDC foram agrupadas em dez classes mensais, entre o 5o e 305o dia da lactação (PLDC1 a PLDC10. Foram realizadas análises considerando 11 modelos diferentes, como segue: multi-característica padrão (MC; cinco modelos de posto reduzido, para a matriz de covariância genética, ajustando um a cinco (CP1 ... CP5 componentes principais; e dois modelos utilizando análise de fatores (F1, F2, F3, F4 e F5. Para todos os modelos, foram considerados como aleatórios os efeitos genético aditivo e o residual e como fixos os de grupo de contemporâneos, da idade da vaca ao parto (linear e quadrático e dias em lactação (linear. Os valores de Log L, AIC e BIC melhoraram com o aumento do número de parâmetros até CP4 e AF4. Comparando CP4 e AF4, observa-se que CP4 resultou em melhores valores de Log L, AIC e BIC. As estimativas de herdabilidade e correlações genéticas utilizando os modelos MC, CP4 e AF4 foram similares, variando de 0,06 (PL6 a 0,65 (PL10 e de 0,05 (PL4xPL10 a 0,94 (PL2xPL3, respectivamente, indicando que a estrutura de covariâncias genéticas entre as produções de leite no dia do controle pode ser ajustada utilizando um modelo de posto reduzido, contendo quatro componentes principais ou quatro fatores

  17. Intraocular Lens Power Estimation in Combined Phacoemulsification and Pars Plana Vitrectomy in Eyes with Epiretinal Membranes: A Case-Control Study

    Science.gov (United States)

    Kim, Min; Kim, Hyoung Eun; Lee, Dong Hyun; Koh, Hyoung Jun; Lee, Sung Chul

    2015-01-01

    Purpose To evaluate the accuracy of postoperative refractive outcomes of combined phacovitrectomy for epiretinal membrane (ERM) in comparison to cataract surgery alone. Materials and Methods Thirty-nine eyes that underwent combined phacovitrectomy with intraocular lens (IOL) implantation for cataract and ERM (combined surgery group) and 39 eyes that received phacoemulsification for cataract (control group) were analyzed, retrospectively. The predicted preoperative refractive aim was compared with the results of postoperative refraction. Results In the combined surgery group, refractive prediction error by A-scan and IOLMaster were -0.305±0.717 diopters (D) and -0.356±0.639 D, respectively, compared to 0.215±0.541 and 0.077±0.529 in the control group, showing significantly more myopic change compared to the control group (p=0.001 and p=0.002, respectively). Within each group, there was no statistically significant difference in refractive prediction error between A-scan and IOLMaster (all p>0.05). IOL power calculation using adjusted A-scan measurement of axial length based on the macular thickness of the normal contralateral eye still resulted in significant postoperative refractive error (all ppower estimation in eyes with cataract and ERM, sequential surgery for ERM and cataract may need to be considered. PMID:25837189

  18. AGARD Conference Proceedings on Guidance and Control of Precision Guided Weapons Held in Geilo, Norway from 3-6 May 1988

    Science.gov (United States)

    1989-01-11

    et lo circuit floctrique Equivalent. aimant permanent aimants permanents /Masse sensible’ piitcos polaires 2-a 2-b 2-c Figure 2 Circuits magndtiques...2-a et 2-b deux rdalisations possiblos 2-c position do Ie masse sensible par rapport eu circuit magnetique et orientation do l’lnduction utile per...passage que le moteur de rappel reprdsentd en d’tail fig.4, fournit en principe une force de rappel proportionnelle au courant le traversant (pas d’erreur

  19. Temperature non-destructive testing by microwave radiometry: reduction of the frequency bandwidth; Controle non destructif de temperature par radiometrie micro-onde a bande etroite

    Energy Technology Data Exchange (ETDEWEB)

    Dubois, L.; Vanoverschelde, C.; Sozanski, J.P.; Chive, M. [IEMN-UMR CNRS 9929, Dept. Hyperfrequences et Semi-Conducteurs, 59 - Villeneuve d' Ascq (France)

    1999-07-01

    Temperature is an important parameter for industrial process control. With the usual methods we obtain only an invasive or superficial information about temperature. Microwave radiometry is a non-invasive way to know the temperature within dissipative body. This paper presents the design of a new radiometer. With this system, the radiometric temperature is independent of the reflection coefficient of the captor. A simplified calibration takes into account insertion losses of the microwave elements. Radiometer frequency bandwidth has been greatly reduced and we present the first results. (authors)

  20. Control vectorial óptimo de accionamientos eléctricos asíncronos con par resistente dependiente de la velocidad

    OpenAIRE

    Rodríguez Arribas, Jaime

    2011-01-01

    Se propone un sistema de regulación de velocidad para motores asíncronos cuyo objetivo es buscar el máximo ahorro energético. Esto se conseguirá a base de reducir al mínimo las pérdidas en el motor-convertidor durante los regímenes permanentes y a base de minimizar el tiempo que duran los procesos transitorios de cambio de un punto de funcionamiento permanente a otro, aplicando la teoría de Control Optimo y teniendo en cuenta que tanto el punto de partida, como el final en el transitorio son ...

  1. Developpement d'un estimateur d'etat energetique d'un chauffe-eau pour un controle par champ moyen

    Science.gov (United States)

    Solis, Jerome

    Domestic electric water heaters (EWH) provide an interesting energy storage potential that can be exploited in the context of smart grids. For example, a variation of 1 °C of the water temperature of an EWH of 270 liters corresponds to 0.313 kWh of energy. For utilities like Hydro-Quebec, which has about 2 million EWH connected to its grid, this represents a strong storage potential. This storage source already present on the network could allow, using an adequate control strategy, to smooth the electricity consumption curve by moving some of the load occurring during peak periods to trough periods. This thesis position itself in the context of developing a simulation platform to implement a mean field control strategy of water heaters on the grid. This innovative approach enables control of all water heaters to meet a global objective while respecting local constraints of each. To be functional, the strategy requires several components to be integrated into the simulation platform. Specifically, this thesis focuses on the development of a numerical model and the estimation of the state in temperature and energy of an EWH. The proposed model for predicting the behavior of a EWH is a multi-nodal model. This approach allows discretizing the tank of an EWH into smaller volumes. The energy conservation equation is solved for each of these sub-volumes and it is possible to predict changes in the output mean temperature with an average error of 2.4 °C. The results from the multi-nodal model compare favorably with numerical reference models, found in the TRNSYS thermal simulation software, as well as experimental measurements of temperature of an electric water heater. In this model, the temperature is assumed to be uniform for each sub-volume and the standby losses are considered constants. Although the model is linear, a correction routine allows redistributing adequately heat within the tank while respecting the thermal dynamics of the heater, including the presence

  2. PAR-1 contributes to the innate immune response during viral infection.

    Science.gov (United States)

    Antoniak, Silvio; Owens, A Phillip; Baunacke, Martin; Williams, Julie C; Lee, Rebecca D; Weithäuser, Alice; Sheridan, Patricia A; Malz, Ronny; Luyendyk, James P; Esserman, Denise A; Trejo, JoAnn; Kirchhofer, Daniel; Blaxall, Burns C; Pawlinski, Rafal; Beck, Melinda A; Rauch, Ursula; Mackman, Nigel

    2013-03-01

    Coagulation is a host defense system that limits the spread of pathogens. Coagulation proteases, such as thrombin, also activate cells by cleaving PARs. In this study, we analyzed the role of PAR-1 in coxsackievirus B3-induced (CVB3-induced) myocarditis and influenza A infection. CVB3-infected Par1(-/-) mice expressed reduced levels of IFN-β and CXCL10 during the early phase of infection compared with Par1(+/+) mice that resulted in higher viral loads and cardiac injury at day 8 after infection. Inhibition of either tissue factor or thrombin in WT mice also significantly increased CVB3 levels in the heart and cardiac injury compared with controls. BM transplantation experiments demonstrated that PAR-1 in nonhematopoietic cells protected mice from CVB3 infection. Transgenic mice overexpressing PAR-1 in cardiomyocytes had reduced CVB3-induced myocarditis. We found that cooperative signaling between PAR-1 and TLR3 in mouse cardiac fibroblasts enhanced activation of p38 and induction of IFN-β and CXCL10 expression. Par1(-/-) mice also had decreased CXCL10 expression and increased viral levels in the lung after influenza A infection compared with Par1(+/+) mice. Our results indicate that the tissue factor/thrombin/PAR-1 pathway enhances IFN-β expression and contributes to the innate immune response during single-stranded RNA viral infection.

  3. Par Pond Fish, Water, and Sediment Chemistry

    Energy Technology Data Exchange (ETDEWEB)

    Paller, M.H. [Westinghouse Savannah River Company, AIKEN, SC (United States); Wike, L.D.

    1996-06-01

    The objectives of this report are to describe the Par Pond fish community and the impact of the drawdown and refill on the community, describe contaminant levels in Par Pond fish, sediments, and water and indicate how contaminant concentrations and distributions were affected by the drawdown and refill, and predict possible effects of future water level fluctuations in Par Pond.

  4. Pars planitis: Epidemiology, clinical characteristics, management and visual prognosis

    Directory of Open Access Journals (Sweden)

    Pinar Cakar Ozdal

    2015-01-01

    Full Text Available Pars planitis is an idiopathic chronic intermediate uveitis which predominantly affects children and adolescents, and accounts for 5-26.7% of pediatric uveitis. Although an autoimmune process with a genetic predisposition has been suggested, its etiology still remains unknown. The most common presenting symptoms are floaters and blurred vision. Diffuse vitreous cells, haze, snowballs and snowbanks are typical findings of pars planitis. Peripheral retinal vasculitis, optic disc edema and anterior segment inflammation are other well-known findings. Although pars planitis is known to be a benign form of uveitis in most cases, it may become a potentially blinding disease due to complications including cataract, cystoid macular edema, vitreous opacities and optic disc edema. Cystoid macular edema is the most common cause of visual morbidity. Band keratopathy, epiretinal membrane formation, vitreous condensation, neovascularizations, vitreous hemorrhage, retinal detachment, cyclitic membranes, glaucoma and amblyopia may develop as a consequence of the chronic course of the disease. Exclusion of infectious and non-infectious causes which may present with intermediate uveitis is of utmost importance before starting treatment. Treatment of pars planitis has been a controversial issue. There is no consensus specifically for treatment of cases with minimal inflammation and relatively good visual acuity. However, current experience shows that pars planitis may cause severe inflammation and needs an aggressive treatment. A stepladder approach including corticosteroids, immunosupressive agents, anti-tumor necrosis factor-alpha and pars plana vitrectomy and/or laser photocoagulation is the most commonly used method for treatment of pars planitis. Adequate control of inflammation and prompt detection of associated complications are crucial in order to improve the overall prognosis of the disease.

  5. PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity.

    Science.gov (United States)

    Fukushima, Henrique; Alves, Vanessa Tubero Euzebio; Carvalho, Verônica Franco de; Ambrósio, Lucas Macedo Batitucci; Eichler, Rosangela Aparecida Dos Santos; Carvalho, Maria Helena Catelli de; Saraiva, Luciana; Holzhausen, Marinella

    2017-01-26

    Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF) of patients with moderate chronic periodontitis (MP). The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19) at baseline, and from MP patients (n = 19) and severe chronic periodontitis (SP) (n = 19) patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA) in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR). PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection.

  6. suPAR: The Molecular Crystal Ball

    Directory of Open Access Journals (Sweden)

    Maria Thunø

    2009-01-01

    Full Text Available soluble urokinase Plasminogen Activator Receptor (suPAR levels reflect inflammation and elevated suPAR levels are found in several infectious diseases and cancer. suPAR exists in three forms; suPARI-III, suPARII-III and suPARI which show different properties due to structural differences. Studies suggest that full-length suPAR is a regulator of uPAR/uPA by acting as uPA-scavenger, whereas the cleaved suPARII-III act as a chemotactic agent promoting the immune response via the SRSRY sequence in the linker-region. This review focus on the various suPAR fragments and their involvement in inflammation and pathogenic processes. We focus on the molecular mechanisms of the suPAR fragments and the link to the inflammatory process, as this could lead to medical applications in infectious and pathological conditions.

  7. Two cases of malignant glaucoma unresolved by pars plana vitrectomy

    Directory of Open Access Journals (Sweden)

    Hosoda Y

    2014-04-01

    Full Text Available Yoshikatsu Hosoda, Tadamichi Akagi, Nagahisa YoshimuraDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanAbstract: Malignant glaucoma, which is characterized by a shallow or flat anterior chamber with high intraocular pressure, can usually be resolved by pars plana vitrectomy with anterior hyaloidectomy. We describe two cases in which malignant glaucoma was refractory to conventional treatment and complete vitrectomy. Case one an 88-year-old woman with pseudoexfoliation glaucoma underwent trabeculotomy and subsequently developed malignant glaucoma. Four months after transient recovery by pars plana vitrectomy, the malignant glaucoma recurred. She underwent peripheral iridectomy and local zonulectomy with successful control of her intraocular pressure. In case two, an 85-year-old man had a history of pseudoexfoliation glaucoma. Seven months after phacoemulsification and intraocular lens implantation, he developed malignant glaucoma that was refractory to pars plana vitrectomy. He underwent peripheral iridectomy, goniosynechialysis and trabectome surgery resulting in the successful control of his intraocular pressure. In rare cases of malignant glaucoma refractive to vitrectomy, peripheral iridectomy with or without local zonulectomy is a reasonable and minimally invasive surgical procedure.Keywords: malignant glaucoma, pars plana vitrectomy, peripheral iridectomy

  8. Dynamic interplay of ParA with the polarity protein, Scy, coordinates the growth with chromosome segregation in Streptomyces coelicolor.

    Science.gov (United States)

    Ditkowski, Bartosz; Holmes, Neil; Rydzak, Joanna; Donczew, Magdalena; Bezulska, Martyna; Ginda, Katarzyna; Kedzierski, Pawel; Zakrzewska-Czerwińska, Jolanta; Kelemen, Gabriella H; Jakimowicz, Dagmara

    2013-03-27

    Prior to bacterial cell division, the ATP-dependent polymerization of the cytoskeletal protein, ParA, positions the newly replicated origin-proximal region of the chromosome by interacting with ParB complexes assembled on parS sites located close to the origin. During the formation of unigenomic spores from multi-genomic aerial hyphae compartments of Streptomyces coelicolor, ParA is developmentally triggered to form filaments along the hyphae; this promotes the accurate and synchronized segregation of tens of chromosomes into prespore compartments. Here, we show that in addition to being a segregation protein, ParA also interacts with the polarity protein, Scy, which is a component of the tip-organizing centre that controls tip growth. Scy recruits ParA to the hyphal tips and regulates ParA polymerization. These results are supported by the phenotype of a strain with a mutant form of ParA that uncouples ParA polymerization from Scy. We suggest that the ParA-Scy interaction coordinates the transition from hyphal elongation to sporulation.

  9. Analyse de l'effet des courants induits sur l'impédance d'un système électromagnétique alimenté en tension BF ou HF. Utilisation de la méthode des circuits couplés

    Science.gov (United States)

    Maouche, B.; Feliachi, M.

    1997-10-01

    In this paper, a study of the interaction between the inductor and the load of an axisymmetrical induction device is proposed. This interaction concerns the effect of the eddy current on both the excitation current and on the system impedance. A half analytical model, based on a numerical discretization of the electromagnetic solution domain, is used. In each cell of the numerical discretization, an analytical calculation using the Moment Method (MM) is considered. In the case of strong skin effect (High Frequency: HF), the formulation makes use of the Impedance Boundary Condition (IBC); in the contrary case (Low Frequency: LF), the interior domain is discretized. Dans cet article nous proposons l'étude de l'influence d'une charge (induit) conductrice sur la répartition du courant inducteur ainsi que sur l'impédance du système. L'inducteur est à géométrie axisymétrique de forme solénoïdale ou pancake destiné au chauffage par induction. Une méthode semi-analytique, basée sur une discrétisation du domaine en mailles élémentaires auxquelles s'applique une formulation intégrale (Méthode des Circuits Couplés : MCC) des grandeurs électromagnétiques, est utilisée. Dans le cas où l'effet de peau est important (Haute Fréquence:HF), la formulation associe la Condition d'Impédance de Surface; dans le cas contraire (Basse Fréquence : BF), un maillage du domaine interne est pratiqué.

  10. Eddy current imaging. Simplifying the direct problem. Analysis of a 2D case with formulations; Imagerie par courants de Foucault. Le probleme direct simplifie. Formulations et etude d`un cas 2D

    Energy Technology Data Exchange (ETDEWEB)

    Spineanu, A.; Zorgati, R.

    1995-12-31

    Eddy current non-destructive testing is used by EDF to detect faults affecting conductive objects such as steam generator tubes. A new technique, known as eddy current imaging, is being developed to facilitate diagnosis in this context. The first stage in this work, discussed in the present paper, consists in solving the direct problem. This entails determining the measurable quantities, on the basis of a thorough knowledge of the material considered. This was done by formulating the direct problem in terms of eddy currents in general 3D geometry context, applying distribution theory and Maxwell equations. Since no direct problem code was available we resorted to simplified situations. Taking care not to interfere with previous developments or those to be attempted in an inversion context, we studied the case of a flaw affecting a 2D structure, illuminated by a plane wave type probe. For this configuration, we studied the exact model and compared results with those of a linearized simplified model. This study emphasizes the ill-posed situation of the eddy current inverse problem related with the severe electromagnetic field attenuation. This means that regularization of the inverse problem, although absolutely necessary, will not be sufficient. Owing to the simplicity of the models available and implemented during the inversion process, processing real data would not yet be possible. We must first focus all our efforts on the direct 3 D problem, in conformity with the requirements of the inverse procedure ad describing a realistic eddy current NDT situation. At the same time, consideration should be given to the design of a specific probe customized for eddy current imaging. (authors). 9 refs., 5 figs., 3 appends.

  11. Eddy current imaging. Limits of the born approximation and advantages of an exact solution to the inverse problem; Imagerie par courants de Foucault. Limites de l`approximation de Born et interet d`une resolution exacte du probleme inverse

    Energy Technology Data Exchange (ETDEWEB)

    Hamman, E.; Zorgati, R.

    1995-12-31

    Eddy current non-destructive testing is used by EDF to detect flaws affecting conductive objects such as steam generator tubes. With a view to obtaining ever more accurate information on equipment integrity, thereby facilitating diagnosis, studies aimed at using measurements to reconstruct an image of the flaw have been proceeding now for about ten years. In this context, our approach to eddy current imaging is based on inverse problem formalism. The direct problem, involving a mathematical model linking measurements provided by a probe with variables characterizing the defect, is dealt with elsewhere. Using the model results, we study the possibility of inverting it, i.e. of reconstructing an image of the flaw from the measurements. We first give an overview of the different inversion techniques, representative of the state of the art and all based on linearization of the inverse problem by means of the Born approximation. The model error resulting from an excessive Born approximation nevertheless severely limits the quantity of the images which can be obtained. In order to counteract this often critical error and extend the eddy current imaging application field, we have to del with the non-linear inverse problem. A method derived from recent research is proposed and implemented to ensure consistency with the exact model. Based on an `optimization` type approach and provided with a convergence theorem, the method is highly efficient. (authors). 17 refs., 7 figs., 1 append.

  12. Study of gas-solid contact in an ultra-rapid reactor for cumene catalytic cracking; Etude du contact gaz-solide dans un reacteur a co-courant descendant par la mise en oeuvre du craquage catalytique du cumene

    Energy Technology Data Exchange (ETDEWEB)

    Bayle, J.

    1996-11-05

    Few studies have been carried out on the notion of gas-solid contact in ultra-rapid reactors. Both gas and solid move in the reactor and the contact can be directly estimated when using a chemical reaction such as cumene cracking. It`s a pure and light feedstock whose kinetics can be determined in a fixed bed. The study was carried out on a downflow ultra-rapid reactor (ID = 20 mm, length = 1 m) at the University of Western Ontario. It proved that the quench and the ultra-rapid separation of gas and solid must be carefully designed in the pilot plant. Cumene conversion dropped when reducing gas-solid contact, which led to push the temperature over 550 deg. C and increase the cat/oil ratio at 25 working at solid mass fluxes below 85 kg/m{sup 2}.s. Change of selectivity at very short residence time were also observed due to deactivation effects. Experiments made by Roques (1994) with phosphorescent pigments on the Residence Time Distribution of solids gave Hydrodynamic data on a cold flow copy of the pilot plant. Experiments made on packed bed gave kinetic data on the cracking of cumene. These data were combined to optimize a mono dimensional plug flow model for cumene cracking. (author)

  13. ParAB Partition Dynamics in Firmicutes: Nucleoid Bound ParA Captures and Tethers ParB-Plasmid Complexes.

    Directory of Open Access Journals (Sweden)

    Virginia S Lioy

    Full Text Available In Firmicutes, small homodimeric ParA-like (δ2 and ParB-like (ω2 proteins, in concert with cis-acting plasmid-borne parS and the host chromosome, secure stable plasmid inheritance in a growing bacterial population. This study shows that (ω:YFP2 binding to parS facilitates plasmid clustering in the cytosol. (δ:GFP2 requires ATP binding but not hydrolysis to localize onto the cell's nucleoid as a fluorescent cloud. The interaction of (δ:CFP2 or δ2 bound to the nucleoid with (ω:YFP2 foci facilitates plasmid capture, from a very broad distribution, towards the nucleoid and plasmid pairing. parS-bound ω2 promotes redistribution of (δ:GFP2, leading to the dynamic release of (δ:GFP2 from the nucleoid, in a process favored by ATP hydrolysis and protein-protein interaction. (δD60A:GFP2, which binds but cannot hydrolyze ATP, also forms unstable complexes on the nucleoid. In the presence of ω2, (δD60A:GFP2 accumulates foci or patched structures on the nucleoid. We propose that (δ:GFP2 binding to different nucleoid regions and to ω2-parS might generate (δ:GFP2 gradients that could direct plasmid movement. The iterative pairing and unpairing cycles may tether plasmids equidistantly on the nucleoid to ensure faithful plasmid segregation by a mechanism compatible with the diffusion-ratchet mechanism as proposed from in vitro reconstituted systems.

  14. Recovering Energy at Entry of Natural Gas into Customer Premises by Employing a Counter-Flow Vortex Tube Récupération d’énergie à l’arrivée du gaz naturel dans les installations des usagers grâce à l’emploi d’un tube Vortex à contre-courant

    Directory of Open Access Journals (Sweden)

    Farzaneh-Gord M.

    2010-10-01

    Full Text Available Throttling valves are currently utilised to reduce high-pressure natural gas flowing through the distribution pipeline to the working level of customers’ equipment. This wastes valuable energy of the gas. Due to low natural gas consumption at customer premises, it is not feasible to utilise expansion machines. In this study, a new idea is proposed to take advantage of the Vortex Tube and natural gas pressure reduction. The idea is to replace the throttling valve with a Vortex Tube in the natural gas pressure reduction system and take advantage of the generated cooling capacity. An experimental investigation was made to determine the effects of the cold orifice diameter and the energy separation of the counter-flow Vortex Tube when air and natural gas are used as the fluid. The energy separation was investigated by use of the experimentally obtained data. La reduction de la pression de gaz naturel entre les conduites de distribution haute pression et les installations des usagers est aujourd’hui assuree par des vannes de reduction de pression. Ce dispositif entraine une perte importante du contenu energetique du gaz. Les installations des usagers consommant trop peu de gaz naturel pour envisager d’avoir recours a des dispositifs d’expansion, notre etude explore les avantages potentiels de l’utilisation d’un tube Vortex pour la reduction de la pression de gaz naturel. Il s’agit de remplacer la vanne de reduction de pression par un tube Vortex dans le dispositif de reduction de la pression de gaz naturel et de tirer profit de la capacite de refroidissement produite. L’etude experimentale avait pour objectif de determiner l’effet du diametre de l’orifice froid ainsi que de l’utilisation de l’air et du gaz naturel comme fluides sur la production d’energie dans le tube Vortex a contre-courant. Le taux de recuperation de l’energie est determine a partir des mesures experimentales.

  15. Effet de l'etat de la surface de l'acier au carbone 300W sur l'entartrage par la gibbsite dans le procede Bayer

    Science.gov (United States)

    Gavril, Liliana

    Une etude a ete effectuee sur l'entartrage des surfaces en acier au carbone par la gibbsite dans le procede Bayer. Trois methodes ont ete utilisees afin de determiner le mecanisme de formation du tartre: mesure de la vitesse d'entartrage, etude electrochimique et protection cathodique. Cette etude demontre que le tartre de gibbsite est un tartre de cristallisation et que l'etat physico-chimique de la surface metallique a une influence marquee sur l'entartrage. Ainsi, l'oxyde en surface a ete identifie comme un facteur determinant pour l'entartrage. Les essais electrochimiques ont montre que la couche d'oxyde formee dans les solutions Bayer est un oxyde mixte de fer et d'aluminium. Les essais de protection cathodique a courant constant valident le role determinant joue par l'oxyde sur l'entartrage, puisqu'en empechant l'oxyde de se former, l'entartrage est pratiquement elimine. Le mecanisme d'adherence du tartre a l'acier implique trois etapes, soit l'oxydation du metal, la formation de la goethite/goethite alumineuse sur l'oxyde et finalement, la germination de la gibbsite sur la goethite/goethite alumineuse.

  16. Aqueous misdirection following pars plana vitrectomy and silicone oil injection

    Directory of Open Access Journals (Sweden)

    Ghoraba HH

    2015-05-01

    Full Text Available Hammouda H Ghoraba,1,2 Ali Ahmed Ghali,3 Hosam Othman Mansour2,3 1Tanta University, Tanta, Egypt; 2Magrabi Eye Hospital, Cairo, Egypt; 3Al-Azhar University, Cairo, Egypt Purpose: To report a retrospective series of seven phakic eyes of seven patients suffering from a malignant glaucoma-like syndrome following pars plana vitrectomy and silicone oil (SO injection.Materials and methods: Seven eyes with retinal detachment treated with pars plana vitrectomy with or without scleral buckling with SO tamponade. This was followed by cataract extraction to manage the elevated intraocular pressure (IOP.Results: This was a retrospective review of seven cases that received pars plana vitrectomy and SO with or without scleral buckling for different causes of retinal detachment (three were rhegmatogenous and four were tractional. After a period ranging from 1 week to 1 month, they presented with malignant glaucoma-like manifestations; high IOP, shallow axial anterior chamber, and remarkable decrease of visual acuity. Atropine eye drops and anti-glaucoma medical treatment (topical and systemic had been tried but failed to improve the condition. Dramatic decrease of IOP and deepening of the axial anterior chamber was observed in all cases in the first postoperative day after phacoemulsification and posterior chamber foldable intraocular lens implantation with posterior capsulotomy.Conclusion: Aqueous misdirection syndrome may be observed following pars plana vitrectomy and SO tamponade. This must be differentiated from other causes of post vitrectomy glaucoma. Cataract extraction with posterior capsulotomy controls the condition. Keywords: malignant glaucoma, pars plana vitrectomy, silicone oil

  17. ¡París!

    Directory of Open Access Journals (Sweden)

    Beatriz Caballero

    2003-01-01

    Full Text Available El día no le alcanzaba para caminarlo. Conocía cada puente, cada calle,plaza, museo, rincón, iglesia, café, y la mesa con el mejor ángulo para mirar siempre una iglesia, ojalá Saint-Germain-des-Pres, o Notre-Dame. Siempre contaba la impresión que le había causado a Bolívar ver allí cuando el papa coronó a Napoleón. Él se iba para la oficina todos los días, Luis a un taller a pintar, Antonio a estudiar ciencias políticas, yo al colegio y mamá hacía las cosas prácticas. Aprendíamos francés todos al tiempo y por la noche nos contábamos lo nuevo que cada uno había aprendido. Mamá después, cuando ya se quería volver, decía que París nos había maleado a todos. Estaba en pleno furor el cine francés de la "nueva ola", pero ella sólo me llevaba a ver películas de vaqueros pues no se fiaba de la clasificación de los periódicos.

  18. Donovanose no Pará

    Directory of Open Access Journals (Sweden)

    Domingos Silva

    1991-12-01

    Full Text Available Foram estudados 259 casos de donovanose registrados no Serviço de Dermatologia do Departamento de Medicina Tropical/UFPA entre 1954-1990. Observa-se que no período 1954-1974 existiam nos arquivos apenas 56 prontuários da doença, enquanto que no último quinquénio estudado (1986-1990 foram identificados 133 casos. Para os autores esse crescimento está ligado a excessiva liberalidade sexual, ao homossexualismo e as precárias condições sócio-econômicas, acentuada nos últimos tempos.From 1954 to 1990 granuloma inguinale was diagnosed in 259 patients al the dermatology service of the Universidade Federal do Pará in Belém, Brazil. Among them, 56 cases had ocurred in the twenty-year period 1954-1974 and as many as 133 cases were seen during the last five years only. Greater sexual liberty, poor social and economic conditions, and especially increasing homosexual behavior were implicated by the authors as chief determinants for this augmented incidence of the disease.

  19. Real-time control strategies for nitrogen removal in WWTP: experimental validation in the Galindo WWTP; Estrategias avanzadas de control automatico par ala eliminacion de N en EDAR: validacion experimental en la EDAR de Galindo

    Energy Technology Data Exchange (ETDEWEB)

    Ayesa Iturrate, E.; Grau Gumbau, P.; Salterain Ezquerra, A.; Irizar Picon, I.

    2007-07-01

    The establishment of rela-time strategies in WWTP biological treatments are able to optimize the operational criteria, to improve the quality of the effluent and to reduce of total operating costs. This paper describes some basic operational criteria for plants with COD and N removal and an advanced control architecture made up of three independent control loops with the aim of optimising nitrogen removal in WWTP. the architecture has been validated experimentally in the Galindo WWTP. Previously, the WEST simulation environment was used in order to analyse by simulation the performance of each control loop. Reduction of total nitrogen removal close to 2 mg N/L and aeration cost savings of 15-20% are the are the results. (Author) 12 refs.

  20. Iduna is a poly(ADP-ribose) (PAR)-dependent E3 ubiquitin ligase that regulates DNA damage

    Science.gov (United States)

    Kang, Ho Chul; Lee, Yun-Il; Shin, Joo-Ho; Andrabi, Shaida A.; Chi, Zhikai; Gagné, Jean-Philippe; Lee, Yunjong; Ko, Han Seok; Lee, Byoung Dae; Poirier, Guy G.; Dawson, Valina L.; Dawson, Ted M.

    2011-01-01

    Ubiquitin mediated protein degradation is crucial for regulation of cell signaling and protein quality control. Poly(ADP-ribose) (PAR) is a cell-signaling molecule that mediates changes in protein function through binding at PAR binding sites. Here we characterize the PAR binding protein, Iduna, and show that it is a PAR-dependent ubiquitin E3 ligase. Iduna’s E3 ligase activity requires PAR binding because point mutations at Y156A and R157A eliminate Iduna’s PAR binding and Iduna’s E3 ligase activity. Iduna’s E3 ligase activity also requires an intact really interesting new gene (RING) domain because Iduna possessing point mutations at either H54A or C60A is devoid of ubiquitination activity. Tandem affinity purification reveals that Iduna binds to a number of proteins that are either PARsylated or bind PAR including PAR polymerase-1, 2 (PARP1, 2), nucleolin, DNA ligase III, KU70, KU86, XRCC1, and histones. PAR binding to Iduna activates its E3 ligase function, and PAR binding is required for Iduna ubiquitination of PARP1, XRCC1, DNA ligase III, and KU70. Iduna’s PAR-dependent ubiquitination of PARP1 targets it for proteasomal degradation. Via PAR binding and ubiquitin E3 ligase activity, Iduna protects against cell death induced by the DNA damaging agent N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest and promotes cell survival after γ-irradiation. Moreover, Iduna facilitates DNA repair by reducing apurinic/apyrimidinic (AP) sites after MNNG exposure and facilitates DNA repair following γ-irradiation as assessed by the comet assay. These results define Iduna as a PAR-dependent E3 ligase that regulates cell survival and DNA repair. PMID:21825151

  1. de larvas de parásitos gastrointestinales de ovinos

    Directory of Open Access Journals (Sweden)

    V\\u00EDctor \\u00C1lvarez C.

    2007-01-01

    Full Text Available Los parásitos gastrointestinales juegan un papel importante en los sistemas de producción animal, particularmente en los ovinos. El desarrollo de resistencia a los productos químicos ha creado un enorme problema para la producción. En busca de nuevas alternativas para el control integrado de parásitos, se utilizó aserrín de: pochote (Bombacopsis quinata, melina (Gmelina arborea, ciprés (Cupressus lusitanica y teca (Tectona grandis en coprocultivos de larvas de parásitos gastrointestinales de ovinos. Se recolectó muestras fecales directamente del recto de los animales en una finca en San José, Costa Rica y se determinó la carga parasitaria. Posteriormente se realizó los coprocultivos, utilizando como control aserrín de laurel (Cordia alliodora. El análisis de la eficacia de cada uno de los aserrines demostró que redujeron el crecimiento de las larvas entre un 65,4 y un 98,0% con relación al control.

  2. Control of the electrical operating point of an electric discharge by mean of its power supply; Controle du point de fonctionnement des decharges electriques par l'intermediaire de leur alimentation

    Energy Technology Data Exchange (ETDEWEB)

    Salanne, J.Ph.

    2005-11-15

    The operating points obtained by the coupling of the power supply with the electric discharge system can be unstable because of the dynamical behaviour of the discharge or because of a change in its length. In this work, the different possible couplings existing between the discharge and the characteristics of its power supply are analyzed in order to optimize the design and control of the power supply and to control the operating point. Analytical and numerical modeling of the system are proposed which allow to simulate the couplings between the power supply and the discharge. This approach is completed by experimental investigations allowing to consider the cases of peak/peak discharges, dielectric barrier discharges (DBD), and gliding arcs. (J.S.)

  3. The urokinase receptor (uPAR facilitates clearance of Borrelia burgdorferi.

    Directory of Open Access Journals (Sweden)

    Joppe W R Hovius

    2009-05-01

    Full Text Available The causative agent of Lyme borreliosis, the spirochete Borrelia burgdorferi, has been shown to induce expression of the urokinase receptor (uPAR; however, the role of uPAR in the immune response against Borrelia has never been investigated. uPAR not only acts as a proteinase receptor, but can also, dependently or independently of ligation to uPA, directly affect leukocyte function. We here demonstrate that uPAR is upregulated on murine and human leukocytes upon exposure to B. burgdorferi both in vitro as well as in vivo. Notably, B. burgdorferi-inoculated C57BL/6 uPAR knock-out mice harbored significantly higher Borrelia numbers compared to WT controls. This was associated with impaired phagocytotic capacity of B. burgdorferi by uPAR knock-out leukocytes in vitro. B. burgdorferi numbers in vivo, and phagocytotic capacity in vitro, were unaltered in uPA, tPA (low fibrinolytic activity and PAI-1 (high fibrinolytic activity knock-out mice compared to WT controls. Strikingly, in uPAR knock-out mice partially backcrossed to a B. burgdorferi susceptible C3H/HeN background, higher B. burgdorferi numbers were associated with more severe carditis and increased local TLR2 and IL-1beta mRNA expression. In conclusion, in B. burgdorferi infection, uPAR is required for phagocytosis and adequate eradication of the spirochete from the heart by a mechanism that is independent of binding of uPAR to uPA or its role in the fibrinolytic system.

  4. Pars Plana Vitrectomy in Advanced Coats’ Disease

    OpenAIRE

    Gulipek Muftuoglu; Gokhan Gulkilik

    2011-01-01

    Purpose: To report 5 cases of advanced Coats’ disease managed with pars plana vitrectomy and silicone oil tamponade. Methods: Five patients with advanced Coats’ disease and serous or tractional retinal detachment underwent pars plana vitrectomy with internal drainage, endolaser photocoagulation and silicone oil tamponade. One patient had combined phacoemulsification-vitrectomy surgery. Of the 5 patients, 1 had intravitreal hemorrhage and a retinal macrocyst and 1 had a retinal cyst. Follow-up...

  5. Soluble Urokinase Plasminogen Activator Receptor (suPAR) is Associated with Metabolic Changes in HIV-1-Infected Africans: A Prospective Study

    DEFF Research Database (Denmark)

    Fourie, Carla M T; Van Rooyen, Johannes M; Olsen, Michael H

    2011-01-01

    and untreated HIV-1-infected Africans compared to uninfected controls at baseline and at a 3-year follow-up, and secondly whether suPAR levels are correlated with cardiovascular and/or metabolic changes. SuPAR, cardiovascular, and metabolic variables were assessed and the percentage change was determined. HIV-1......-infected black South Africans had significantly higher suPAR levels than uninfected controls at baseline and at follow-up 3 years later. However, only the treated HIV-1-infected participants showed an increase in suPAR levels at follow-up. The treated group also showed signs of lipodystrophy...... and their baseline suPAR levels correlated positively with an increased waist circumference. This study indicates that suPAR levels increase and that baseline suPAR is associated with an increase in abdominal fat distribution in HIV-infected black Africans on antiretroviral therapy....

  6. CERCLA interim action at the Par Pond unit: A case study

    Energy Technology Data Exchange (ETDEWEB)

    Hickey, H.M. [Westinghouse Savannah River Co., Aiken, SC (United States); Matthews, S.S.; Neal, L.W. [Rust Environment and Infrastructure, Inc., Greenville, SC (United States); Weiss, W.R. [Rust Environment and Infrastructure, Inc., Aiken, SC (United States)

    1993-11-01

    The Par Pond unit designated under CERCLA consists of sediments within a Savannah River Site (SRS) cooling water reservoir. The sediments are contaminated with radionuclides and nonradioactive constituents from nuclear production reactor operations. The mercury in Par Pond is believed to have originated from the Savannah River. Because of Par Pond Dam safety Issues, the water level of the reservoir was drawn down, exposing more than 1300 acres of contaminated sediments and triggering the need for CERCLA interim remedial action. This paper presents the interim action approach taken with Par Pond as a case study. The approach considered the complexity of the Par Pond ecosystem, the large size of Par Pond, the volume of contaminated sediments, and the institutional controls existing at SRS. The Environmental Protection Agency (EPA) considers units with large volumes of low-concentration wastes, as is the case with Par Pond, to be {open_quotes}special sites.{close_quotes} Accordingly, EPA guidance establishes that the range of alternatives developed focus primarily on containment options and other remedial approaches that mitigate potential risks associated with the {open_quotes}special site.{close_quotes} The remedial alternatives, according to EPA, are not to be prohibitively expensive or difficult to implement. This case study also is representative of the types of issues that will need to be addressed within the Department of Energy (DOE) complex as nuclear facilities are transitioned to inactive status and corrective/remedial actions are warranted.

  7. Effects of solar PAR and UV radiation on tropical biofouling communities

    KAUST Repository

    Dobretsov, SV

    2010-03-08

    We investigated the effect of solar ultraviolet radiation (UVR) and photosynthetically active radiation (PAR) on the development of tropical micro- and macrofouling communities for 30 d. The experimental design involved 3 treatments: full spectrum (PAR+UVR), PAR only, and minimal light (reduced PAR and UVR). Terminal restriction fragment length polymorphism analysis demonstrated that different light conditions resulted in the formation of highly different microbial communities. The lowest densities of bacteria were found under the full spectrum treatment, while the lowest densities of diatoms were found in the minimal light treatment. Macrofouling communities consisted of 13 species and differed among light treatments. In the presence of UVR, communities had low species diversity, evenness, and richness, while in minimal light and PAR treatments, communities had high species diversity, evenness, and richness. Similarity percentage (SIMPER) analysis revealed that the tubeworm Hydroides elegans, the alga Ulva (Enteromorpha) sp., and the bivalve Perna viridis were the species responsible for most of the dissimilarities in macrofouling communities among treatments. While densities of H. elegans were similar in the PAR and minimal light treatments, this polychaete had higher growth rates under minimal light conditions. We conclude that UVR and PAR directly control the development of shallow micro- and macrofouling communities by inhibiting the recruitment and growth of sensitive species and promoting the growth of resistant species, but also that these forms of solar radiation influence the surface cues available to competent larvae by altering the development of the microbial community.

  8. La diversité vue par un journal communautaire maghrébin à Montréal

    Directory of Open Access Journals (Sweden)

    Rachad Antonius

    2009-01-01

    Full Text Available Cet article se situe dans le champ d’étude des médias “communautaires” ou ethniques. Il analyse la couverture, par le journal communautaire maghrébin Atlas.Mtl, de la question de la diversité au Québec. L’analyse montre qu’Atlas.Mtl s’inscrit dans une logique de négociation des rapports de force entre les citoyens d’origine maghrébine et le groupe majoritaire, dans le cadre d’un système hégémonique dont il ne conteste pas les fondements. Son approche est non conflictuelle, et le journal adopte un ton généralement conciliant qui ne rejette pas globalement les avantages d’un système libéral et laïque, contrastant ainsi avec d’autres journaux plus radicaux dans leurs revendications. On y trouve une ouverture à la diversité d’opinions. En dehors des périodes de tension sociale où le discours sur l’immigration et sur la diversité s’est durci, le contenu du journal revient à un ton moins revendicateur. Son apport se situe davantage au niveau de l’information qu’à celui de la revendication, avec une préoccupation claire pour la modification des situations d’inégalité. Il s’en dégage une orientation générale qui propose une identité arabo-musulmane laïque, modérée et libérale, spécifiquement maghrébine, assumant son appartenance à une société québécoise laïque, et faisant partie de la diaspora internationale maghrébine. Nous situerons cette orientation dans les débats entre le courant des études post-coloniales et les courants qui insistent sur les orientations économiques et intégrationnistes des médias ethniques minoritaires, et nous proposerons des interprétations du positionnement du journal qui font référence au grand débat entre islamisme et nationalisme laïque dans les pays arabes.

  9. C9.A/14 steelwork joints de poutres par plaque frontale : assemblages par gousset

    CERN Document Server

    2015-01-01

    Les Tables de résistances ultimes des assemblages boulonnés par plaque frontale et par gousset, complétées par une description des modèles de calcul et des exemples d’application, ont pour but de faciliter la tâche de l'ingénieur et du constructeur. Cette première partie C9.A/14 contient les chapitres suivants: - Joints de poutres par plaque frontale en acier S235 et S355 - Assemblages par gousset en acier S235 et S355 Les Tables contiennent des données relatives à la géométrie ainsi que les valeurs de calcul correspondantes des résistances ultimes des assemblages ; elles remplacent le chapitre « Assemblages par plaques frontales et boulons HR » des anciennes Tables C9.1 de 1983 / 2002. Le calcul de ces assemblages par plaque frontale est basé sur les hypothèses du modèle de la méthode des composants décrite dans la norme SN EN 1993-1-8. Les vérifications sont effectuées selon la norme SIA 263:2013. Les assemblages par gousset remplacent les assemblages par double cornière, (telle...

  10. Transient early neurotrophin release and delayed inflammatory cytokine release by microglia in response to PAR-2 stimulation.

    Science.gov (United States)

    Chen, Chen-Wen; Chen, Qian-Bo; Ouyang, Qing; Sun, Ji-Hu; Liu, Fang-Ting; Song, Dian-Wen; Yuan, Hong-Bin

    2012-06-25

    Activated microglia exerts both beneficial and deleterious effects on neurons, but the signaling mechanism controlling these distinct responses remain unclear. We demonstrated that treatment of microglial cultures with the PAR-2 agonist, 2-Furoyl-LIGRLO-NH2, evoked early transient release of BDNF, while sustained PAR-2 stimulation evoked the delayed release of inflammatory cytokines (IL-1 β and TNF-α) and nitric oxide. Culture medium harvested during the early phase (at 1 h) of microglial activation induced by 2-Furoyl-LIGRLO-NH2 (microglial conditioned medium, MCM) had no deleterious effects on cultured neurons, while MCM harvested during the late phase (at 72 h) promoted DNA fragmentation and apoptosis as indicated by TUNEL and annexin/PI staining. Blockade of PAR-1 during the early phase of PAR-2 stimulation enhanced BDNF release (by 11%, small but significant) while a PAR-1 agonist added during the late phase (24 h after 2-Furoyl-LIGRLO-NH2 addition) suppressed the release of cytokines and NO. The neuroprotective and neurotoxic effects of activated microglial exhibit distinct temporal profiles that are regulated by PAR-1 and PAR-2 stimulation. It may be possible to facilitate neuronal recovery and repair by appropriately timed stimulation and inhibition of microglial PAR-1 and PAR-2 receptors.

  11. Endothelial progenitor cell-dependent angiogenesis requires localization of the full-length form of uPAR in caveolae.

    Science.gov (United States)

    Margheri, Francesca; Chillà, Anastasia; Laurenzana, Anna; Serratì, Simona; Mazzanti, Benedetta; Saccardi, Riccardo; Santosuosso, Michela; Danza, Giovanna; Sturli, Niccolò; Rosati, Fabiana; Magnelli, Lucia; Papucci, Laura; Calorini, Lido; Bianchini, Francesca; Del Rosso, Mario; Fibbi, Gabriella

    2011-09-29

    Endothelial urokinase-type plasminogen activator receptor (uPAR) is thought to provide a regulatory mechanism in angiogenesis. Here we studied the proangiogenic role of uPAR in endothelial colony-forming cells (ECFCs), a cell population identified in human umbilical blood that embodies all of the properties of an endothelial progenitor cell matched with a high proliferative rate. By using caveolae-disrupting agents and by caveolin-1 silencing, we have shown that the angiogenic properties of ECFCs depend on caveolae integrity and on the presence of full-length uPAR in such specialized membrane invaginations. Inhibition of uPAR expression by antisense oligonucleotides promoted caveolae disruption, suggesting that uPAR is an inducer of caveolae organization. Vascular endothelial growth factor (VEGF) promoted accumulation of uPAR in ECFC caveolae in its undegraded form. We also demonstrated that VEGF-dependent ERK phosphorylation required integrity of caveolae as well as caveolar uPAR expression. VEGF activity depends on inhibition of ECFC MMP12 production, which results in impairment of MMP12-dependent uPAR truncation. Further, MMP12 overexpression in ECFC inhibited vascularization in vitro and in vivo. Our data suggest that intratumor homing of ECFCs suitably engineered to overexpress MMP12 could have the chance to control uPAR-dependent activities required for tumor angiogenesis and malignant cells spreading.

  12. Microhabitats utilisés par les alevins 0+ d'une communauté de cyprins rhéophiles : analyse de l'évolution de la communauté et des densités de population

    Directory of Open Access Journals (Sweden)

    BARAS E.

    1995-04-01

    Full Text Available L'évolution de l'occupation des microhabitats par les alevins de la communauté de cyprins rhéophiles de l'Ourthe a été étudiée au cours de l'été et de l'automne 1994 par échantillonnage ponctuel d'abondance (électrodes fixes prépositionnées, courant continu. 16155 poissons (10-75 mm appartenant à 15 espèces ont été recensés sur 78 sites d'échantillonnage de 2 m2 . Les espèces les plus représentées étaient Leuciscus cephalus, Leuciscus leuciscus et Chondrostoma nasus (respectivement 43,6 %, 20,7 % et 16,7 % des captures estivales. L'analyse factorielle des correspondances d'une matrice site x (espèce x taille met en évidence une subdivision progressive de la communauté au cours de l'été en espèces d'eau vive (B. barbus et L. leuciscus et d'eau lente, davantage associées à un couvert végétal (L. cephalus et C. nasus. La densité maximale observée (fin juin est de 1500 alevins (25 mm m-2, correspondant à une biomasse de 250 g m-2. Le modèle explicatif de la densité généré par analyse de régression multiple pas-à-pas explique 60,0 % de la variabilité de la densité par les variables vitesse de courant, température de l'eau et date d'échantillonnage, cette variable intégrant le concept de mortalité-dispersion au cours de la saison de croissance. Les implications de la méthodologie et des résultats présentés au plan de la gestion des écosystèmes lotiques sont brièvement discutés.

  13. Sociologie de l’alimentation : les cinq portes de l’entrée par les familles 

    Directory of Open Access Journals (Sweden)

    Nicolas Belorgey

    2011-07-01

    Full Text Available La sociologie de l’alimentation s’est peu à peu autonomisée par rapport à la sociologie de la famille. Les deux corpus ne tirent pas aujourd'hui tout le parti de ce qu’ils ont en commun. Ce texte propose un inventaire des courants de recherche, sociologiques ou non, qui s’intéressent à la famille et qui fournissent des éléments théoriques mobilisables ou objectivables pour la sociologie de l’alimentation. On peut identifier ainsi cinq grands courants : l’approche statistique fondée sur les catégories socio-professionnelles, les études de psychologie sociale, les études ethnographiques sur les familles, celles croisant enfance, adolescence et alimentation, enfin celles, à dominante psychanalytique, des origines familiales des troubles de l’alimentation. La confrontation entre ces différents courants permet d’esquisser une méthode de recherche.Sociology of food: the five entry doors by familySociology of food, historically rooted in the sociology of family, has become more independent. The two corpus nowadays do not make full profit of what they have in common. This paper is a survey of the current research trends on family (inside and outside sociology that may be of use for the sociology of food or be part of its research objects. Five trends are distinguished: the statistical approach based on socioprofesionnal status, the social psychology, the ethnographic studies on families, the studies at the intersection of family, childhood, adolescence and food, and the studies of the familial sources of the troubles in the nutritional behavior.Sociología de la alimentación: las cinco puertas de entrada de las familiasLa sociología de la alimentación se ha progresivamente autonomizado de la sociología de la familia. Pero tienen cosas en común de las cuales no se ha sacado todo el provecho debido. Este texto expone un inventario de las tendencias de la investigación, sociológica o no, que tienen como objeto la

  14. Efecto y control del ruido en la determinación experimental de parámetros dinámicos de máquinas síncronas

    OpenAIRE

    Laura L. Juárez-Caltzontzin; Tomás I. Asiaín-Olivares; Daniel Ruiz-Vega

    2011-01-01

    El propósito de este trabajo es presentar el diseño de un filtro digital para eliminar las componentes de alta frecuencia de las corrientes de fase obtenidas a partir de la prueba de corto circuito trifásico con la máquina síncrona operando en vacío. En este trabajo se utilizó el programa de computadora digital ParametrosMS para obtener los parámetros dinámicos de la máquina síncrona [1], [2]. Con el se obtuvieron resultados satisfactorios en el análisis de los oscilogramas de corrientes el...

  15. ParCAT: Parallel Climate Analysis Toolkit

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Brian E. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Steed, Chad A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Shipman, Galen M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Ricciuto, Daniel M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Thornton, Peter E. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Wehner, Michael [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Williams, Dean N. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2013-01-01

    Climate science is employing increasingly complex models and simulations to analyze the past and predict the future of Earth s climate. This growth in complexity is creating a widening gap between the data being produced and the ability to analyze the datasets. Parallel computing tools are necessary to analyze, compare, and interpret the simulation data. The Parallel Climate Analysis Toolkit (ParCAT) provides basic tools to efficiently use parallel computing techniques to make analysis of these datasets manageable. The toolkit provides the ability to compute spatio-temporal means, differences between runs or differences between averages of runs, and histograms of the values in a data set. ParCAT is implemented as a command-line utility written in C. This allows for easy integration in other tools and allows for use in scripts. This also makes it possible to run ParCAT on many platforms from laptops to supercomputers. ParCAT outputs NetCDF files so it is compatible with existing utilities such as Panoply and UV-CDAT. This paper describes ParCAT and presents results from some example runs on the Titan system at ORNL.

  16. Economic-statistical design of variable parameters non-central chi-square control chart Projeto econômico-estatístico de gráficos de controle qui-quadrado não-central com parâmetros variáveis

    Directory of Open Access Journals (Sweden)

    Maysa Sacramento de Magalhães

    2011-06-01

    Full Text Available Production processes are monitored by control charts since their inception by Shewhart (1924. This surveillance is useful in improving the production process due to increased stabilization of the process, and consequently standardization of the output. Control charts keep track of a few key quality characteristics of the outcome of the production process. This is done by means of univariate or multivariate charts. Small improvements in control chart methodology can have significant economic impact in the production process. In this investigation, we propose the monitoring of a single variable by means of a variable parameter non-central chi-square control chart. The design of the chart is accomplished by means of optimizing a cost function. We use here a simulated annealing optimization tool, due to the difficulty of classical gradient based optimization techniques to handle the optimization of the cost function. The results show some of the drawbacks of using this model.Processos de produção são monitorados por gráficos de controle desde a sua introdução por Shewhart (1924. Este monitoramento é útil na melhoria do processo de produção devido à crescente estabilização do processo, e consequentemente, padronização do produto. Gráficos de controle mantêm vigilância de características de qualidade de um processo de produção. Isto é feito por intermédio de gráficos univariados ou multivariados. Melhorias na metodologia de gráficos de controle podem levar a um impacto econômico significativo no processo de produção. Neste artigo, propomos um gráfico de controle de parâmetros variáveis baseado na estatística qui-quadrado nãocentral para monitorar uma característica de qualidade de interesse. O projeto do gráfico é realizado através da otimização de uma função custo. O algoritmo simulated annealing é usado devido à dificuldade dos métodos clássicos de otimização baseados no gradiente, de lidarem com a

  17. Bibliographies africanistes courantes: une comparaison

    Directory of Open Access Journals (Sweden)

    M. d'Hertefelt

    1991-01-01

    Full Text Available Five internationally used current bibliographies are here compared with respect to their coverage of the periodical literature, the degree of their overlapping, and three basic aspects of their layout (list of analyzed periodicals, arrangement of references, index construction. As regards coverage, the most striking conclusion is the low to minimal rate of overlapping, so that it would be rather risky for the student to rely on one bibliography only, even the most extensive. Coverage, of course, is not the sole factor in assessing the value of a bibliography. The five bibliographies differ widely in their organisation and are by far not equally easy to use.

  18. Avaliação de parâmetros clínicos e hematológicos de eqüinos submetidos a um programa de controle estratégico de Amblyomma cajennense (Fabricius, 1787 (Acari: Ixodidae Evaluation of clinical and hematological parameters of equines submitted to a strategic control program of Amblyomma cajennense (Fabricius, 1787 (Acari: Ixodidae

    Directory of Open Access Journals (Sweden)

    A.P. Cunha

    2008-02-01

    Full Text Available Estudaram-se aspectos clínicos e hematológicos em eqüinos submetidos a um programa de controle estratégico de A. cajennense. Os tratamentos carrapaticidas foram realizados a cada sete dias e divididos em dois módulos, o primeiro com início em abril de 2004, e o segundo com início em julho do mesmo ano, utilizando-se a base química piretróide - cipermetrina na concentração de 0,015%. Além do acompanhamento clínico dos animais, foram realizados hemogramas completos antes e após o programa. As dosagens bioquímicas de bilirrubinas, gama-glutamiltransferase (GGT, aspartato aminotransferase (AST, creatina kinase (CK, proteína total, albumina e globulinas, foram realizadas antes, durante e ao final do programa. Os resultados demonstraram que houve uma melhora no quadro hematológico dos animais após o programa de controle. Os tratamentos carrapaticidas, na forma em foram aplicados, não provocaram alterações desfavoráveis nos parâmetros clínicos e hematológicos dos eqüinos. Tais informações podem ser consideradas na busca de alternativas viáveis e seguras para o controle dessa espécie de carrapato.Clinical and hematological parameters were studied in equines submitted to a strategic control program of Amblyomma cajennense. The acaricide treatments were carried to each seven days and divided in two batteries, the first one began in April 2004 and the second in July 2004. A pyrethroid chemical base - 0.015% cypermethrin was used. Clinical examinations of the animals and complete hemograms were carried before and after the control program of the tick. Seric dosages of bilirrubins, gamma-glutamiltransferase (GGT, aspartate aminotransferase (AST, creatin kinase (CK, total protein, albumin, and globulins, were carried before and throughout the experiment. The results showed an improvement in the hematological parameters of the animals after the end of control program. The acaricide treatments did not cause undesirable alterations

  19. Etk/Bmx regulates proteinase-activated-receptor1 (PAR1 in breast cancer invasion: signaling partners, hierarchy and physiological significance.

    Directory of Open Access Journals (Sweden)

    Irit Cohen

    Full Text Available BACKGROUND: While protease-activated-receptor 1 (PAR(1 plays a central role in tumor progression, little is known about the cell signaling involved. METHODOLOGY/PRINCIPAL FINDINGS: We show here the impact of PAR(1 cellular activities using both an orthotopic mouse mammary xenograft and a colorectal-liver metastasis model in vivo, with biochemical analyses in vitro. Large and highly vascularized tumors were generated by cells over-expressing wt hPar1, Y397Z hPar1, with persistent signaling, or Y381A hPar1 mutant constructs. In contrast, cells over-expressing the truncated form of hPar1, which lacks the cytoplasmic tail, developed small or no tumors, similar to cells expressing empty vector or control untreated cells. Antibody array membranes revealed essential hPar1 partners including Etk/Bmx and Shc. PAR(1 activation induces Etk/Bmx and Shc binding to the receptor C-tail to form a complex. Y/A mutations in the PAR(1 C-tail did not prevent Shc-PAR(1 association, but enhanced the number of liver metastases compared with the already increased metastases obtained with wt hPar1. We found that Etk/Bmx first binds via the PH domain to a region of seven residues, located between C378-S384 in PAR(1 C-tail, enabling subsequent Shc association. Importantly, expression of the hPar1-7A mutant form (substituted A, residues 378-384, which is incapable of binding Etk/Bmx, resulted in inhibition of invasion through Matrigel-coated membranes. Similarly, knocking down Etk/Bmx inhibited PAR(1-induced MDA-MB-435 cell migration. In addition, intact spheroid morphogenesis of MCF10A cells is markedly disrupted by the ectopic expression of wt hPar1. In contrast, the forced expression of the hPar1-7A mutant results in normal ball-shaped spheroids. Thus, by preventing binding of Etk/Bmx to PAR(1 -C-tail, hPar1 oncogenic properties are abrogated. CONCLUSIONS/SIGNIFICANCE: This is the first demonstration that a cytoplasmic portion of the PAR(1 C-tail functions as a scaffold

  20. Effect of a physical activity intervention on suPAR levels

    DEFF Research Database (Denmark)

    Rohde, Christopher; Polcwiartek, Christoffer; Andersen, Eivind

    2017-01-01

    : suPAR and C-reactive protein (CRP) levels were determined in blood samples from a previous randomized controlled trial with Pakistani immigrants in Norway, 2008. The study included physically inactive men that were randomized to an intervention group (supervised group exercises) or a control group...... and followed for 5 months. A linear regression model was used and adjusted for age, inactivity level at baseline, and mean difference in CRP levels. RESULTS: Overall, 80 and 53 participants were included in the intervention and control group, respectively. Obesity and smoking were associated with higher su......PAR levels at baseline. The intervention group had a mean suPAR level of 2.65 (95% CI=2.48-2.78)ng/mL at baseline compared to 2.80 (95% CI=2.65-2.95)ng/mL at post-test, and thereby significantly increased suPAR levels after intervention (p=0.02). In the control group, mean suPAR level significantly increased...

  1. Structural analysis of the ParR/parC plasmid partition complex

    DEFF Research Database (Denmark)

    Møller-Jensen, Jakob; Ringgaard, Simon; Mercogliano, Christopher P

    2007-01-01

    Accurate DNA partition at cell division is vital to all living organisms. In bacteria, this process can involve partition loci, which are found on both chromosomes and plasmids. The initial step in Escherichia coli plasmid R1 partition involves the formation of a partition complex between the DNA...... and biochemical experiments support a structural arrangement in which the centromere-like parC DNA is wrapped around a ParR protein scaffold. This structure holds implications for how ParM polymerization drives active DNA transport during plasmid partition....

  2. uPA/uPAR system activation drives a glycolytic phenotype in melanoma cells.

    Science.gov (United States)

    Laurenzana, Anna; Chillà, Anastasia; Luciani, Cristina; Peppicelli, Silvia; Biagioni, Alessio; Bianchini, Francesca; Tenedini, Elena; Torre, Eugenio; Mocali, Alessandra; Calorini, Lido; Margheri, Francesca; Fibbi, Gabriella; Del Rosso, Mario

    2017-09-15

    In this manuscript, we show the involvement of the uPA/uPAR system in the regulation of aerobic glycolysis of melanoma cells. uPAR over-expression in human melanoma cells controls an invasive and glycolytic phenotype in normoxic conditions. uPAR down-regulation by siRNA or its uncoupling from integrins, and hence from integrin-linked tyrosine kinase receptors (IL-TKRs), by an antagonist peptide induced a striking inhibition of the PI3K/AKT/mTOR/HIF1α pathway, resulting into impairment of glucose uptake, decrease of several glycolytic enzymes and of PKM2, a checkpoint that controls metabolism of cancer cells. Further, binding of uPA to uPAR regulates expression of molecules that govern cell invasion, including extracellular matrix metallo-proteinases inducer (EMPPRIN) and enolase, a glycolytyc enzyme that also serves as a plasminogen receptor, thus providing a common denominator between tumor metabolism and phenotypic invasive features. Such effects depend on the α5β1-integrin-mediated uPAR connection with EGFR in melanoma cells with engagement of the PI3K-mTOR-HIFα pathway. HIF-1α trans-activates genes whose products mediate tumor invasion and glycolysis, thus providing the common denominator between melanoma metabolism and its invasive features. These findings unveil a unrecognized interaction between the invasion-related uPAR and IL-TKRs in the control of glycolysis and disclose a new pharmacological target (i.e., uPAR/IL-TKRs axis) for the therapy of melanoma. © 2017 UICC.

  3. PAR-4/LKB1 regulates DNA replication during asynchronous division of the early C. elegans embryo

    Science.gov (United States)

    Descoteaux, Catherine; Chartier, Nicolas T.; Pintard, Lionel; Labbé, Jean-Claude

    2014-01-01

    Regulation of cell cycle duration is critical during development, yet the underlying molecular mechanisms are still poorly understood. The two-cell stage Caenorhabditis elegans embryo divides asynchronously and thus provides a powerful context in which to study regulation of cell cycle timing during development. Using genetic analysis and high-resolution imaging, we found that deoxyribonucleic acid (DNA) replication is asymmetrically regulated in the two-cell stage embryo and that the PAR-4 and PAR-1 polarity proteins dampen DNA replication dynamics specifically in the posterior blastomere, independently of regulators previously implicated in the control of cell cycle timing. Our results demonstrate that accurate control of DNA replication is crucial during C. elegans early embryonic development and further provide a novel mechanism by which PAR proteins control cell cycle progression during asynchronous cell division. PMID:24841566

  4. Prevalence of the subclinical sinus disease in HIV positive patients evaluated by the computed tomography versus a control population; Prevalence de la pathologie sinusienne infra-clinique chez les patients infectes par le VIH etudiee par la tomodensitometrie avec comparaison a une polulation temoin

    Energy Technology Data Exchange (ETDEWEB)

    Senneville, E.; Valette, M.; Ajana, F.; Gerard, Y.; Alfandari, S.; Chidiac, C.; Mouton, Y. [Service universitaire regional centre hospitalier de Tourcoing (France)

    1997-07-01

    To determine the prevalence of subclinical sinus disease in patients infected with the human immunodeficiency virus (HIV), cerebral computed tomography scans (CCT) done at the Tourcoing hospital over an 18-month period in 139 HIV-positive patients and 140 control patients without evidence of active sinus disease were reviewed retrospectively. CCTs were evaluated independently by two physicians who were blinded to clinical data. Mucosal thickening and/or a full patients (20/139, 14.4%) than in the controls (8/140, 5.7%) (p=0.016). Mucosal thickening was the most common abnormality in both groups. CD4+cell counts were not correlated with the radiographic abnormalities studies. These radiographic data suggest that subclinical chronic sinusitis independent from the degree of immune deficiency may be more common in HIV-positive than in HIV-negative subjects. (author). 14 refs.

  5. Pars plana Baerveldt tube insertion with pars plana vitrectomy for refractory glaucoma

    Directory of Open Access Journals (Sweden)

    Anton M Kolomeyer

    2012-01-01

    Conclusions: Pars plana Baerveldt tube implantation with PPV can preserve vision, reduce IOP, and decrease the number of glaucoma medications necessary to achieve target IOP in patients with recalcitrant glaucoma.

  6. PAR Corneal Topography System (PAR CTS): the clinical application of close-range photogrammetry.

    Science.gov (United States)

    Belin, M W; Cambier, J L; Nabors, J R; Ratliff, C D

    1995-11-01

    The PAR Corneal Topography System (CTS) is a computer-driven corneal imaging system which uses close-range photogrammetry (rasterphotogrammetry) to measure and produce a topographic map of the corneal surface. The PAR CTS makes direct point-by-point measurements of surface elevation using a stereo-triangulation technique. The CTS uses a grid pattern composed of horizontal and vertical lines spaced about 0.2 mm (200 microns) apart. Each grid intersection comprises a surface feature which can be located in multiple images and used to generate an (x,y,z) coordinate. Unlike placido disc-based videokeratoscopes, the PAR CTS requires neither a smooth reflective surface nor precise spatial alignment for accurate imaging. In addition to surface elevation, the PAR CTS computes axial and tangential curvatures and refractive power. Difference maps are available in all curvatures, refractive power, and in absolute elevation.

  7. Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells

    Science.gov (United States)

    Hansen, Line; Unmack Larsen, Esben Kjær; Nielsen, Erik Holm; Iversen, Frank; Liu, Zhuo; Thomsen, Karen; Pedersen, Michael; Skrydstrup, Troels; Nielsen, Niels Chr.; Ploug, Michael; Kjems, Jørgen

    2013-08-01

    Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific targeting peptide onto polyethylene glycol (PEG) coated USPIO nanoparticles by click chemistry resulted in a five times higher uptake in vitro in a uPAR positive cell line compared to nanoparticles carrying a non-binding control peptide. In accordance with specific receptor-mediated recognition, a low uptake was observed in the presence of an excess of ATF, a natural ligand for uPAR. The uPAR specific magnetic nanoparticles can potentially provide a useful supplement for tumor patient management when combined with MRI and drug delivery.Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific

  8. A Combined Global and Local Approach to Elucidate Spatial Organization of the Mycobacterial ParB-parS Partition

    Energy Technology Data Exchange (ETDEWEB)

    Chaudhuri, Barnali [University of Buffalo, The State University of New York; Gupta, Sayan [Case Western Reserve University; Urban, Volker S [ORNL; Chance, Mark [Case Western Reserve University; D' Mello, Rhijuta [Case Western Reserve University; Smith, Lauren [University of Buffalo, The State University of New York; Lyons, Kelly [University of Buffalo, The State University of New York; Gee, Jessica [University of Buffalo, The State University of New York

    2010-01-01

    Combining diverse sets of data at global (size, shape) and local (residue) scales is an emerging trend for elucidating the organization and function of the cellular assemblies. We used such a strategy, combining data from X-ray and neutron scattering with H/D-contrast variation and X-ray footprinting with mass spectrometry, to elucidate the spatial organization of the ParB-parS assembly from Mycobacterium tuberculosis. The ParB-parS participates in plasmid and chromosome segregation and condensation in predivisional bacterial cells. ParB polymerizes around the parS centromere(s) to form a higher-order assembly that serves to recruit cyto-skeletal ParA ATPases and SMC proteins for chromosome segregation. A hybrid model of the ParB-parS was built by combining and correlating computational models with experiment-derived information about size, shape, position of the symmetry axis within the shape, internal topology, DNA-protein interface, exposed surface patches, and prior knowledge. This first view of the ParB-parS leads us to propose how ParB spread on the chromosome to form a larger assembly.

  9. A Combined Global and Local Approach to Elucidate Spatial Organization of the Mycobacterial ParB-parS Partition Assembly

    Energy Technology Data Exchange (ETDEWEB)

    B Chaudhuri; S Gupta; V Urban; M Chance; R DMello; L Smith; K Lyons; J Gee

    2011-12-31

    Combining diverse sets of data at global (size, shape) and local (residue) scales is an emerging trend for elucidating the organization and function of the cellular assemblies. We used such a strategy, combining data from X-ray and neutron scattering with H/D-contrast variation and X-ray footprinting with mass spectrometry, to elucidate the spatial organization of the ParB-parS assembly from Mycobacterium tuberculosis. The ParB-parS participates in plasmid and chromosome segregation and condensation in predivisional bacterial cells. ParB polymerizes around the parS centromere(s) to form a higher-order assembly that serves to recruit cyto-skeletal ParA ATPases and SMC proteins for chromosome segregation. A hybrid model of the ParB-parS was built by combining and correlating computational models with experiment-derived information about size, shape, position of the symmetry axis within the shape, internal topology, DNA-protein interface, exposed surface patches, and prior knowledge. This first view of the ParB-parS leads us to propose how ParB spread on the chromosome to form a larger assembly.

  10. A combined global and local approach to elucidate spatial organization of the Mycobacterial ParB-parS partition assembly.

    Science.gov (United States)

    Chaudhuri, Barnali N; Gupta, Sayan; Urban, Volker S; Chance, Mark R; D'Mello, Rhijuta; Smith, Lauren; Lyons, Kelly; Gee, Jessica

    2011-03-22

    Combining diverse sets of data at global (size, shape) and local (residue) scales is an emerging trend for elucidating the organization and function of the cellular assemblies. We used such a strategy, combining data from X-ray and neutron scattering with H/D-contrast variation and X-ray footprinting with mass spectrometry, to elucidate the spatial organization of the ParB-parS assembly from Mycobacterium tuberculosis. The ParB-parS participates in plasmid and chromosome segregation and condensation in predivisional bacterial cells. ParB polymerizes around the parS centromere(s) to form a higher-order assembly that serves to recruit cyto-skeletal ParA ATPases and SMC proteins for chromosome segregation. A hybrid model of the ParB-parS was built by combining and correlating computational models with experiment-derived information about size, shape, position of the symmetry axis within the shape, internal topology, DNA-protein interface, exposed surface patches, and prior knowledge. This first view of the ParB-parS leads us to propose how ParB spread on the chromosome to form a larger assembly.

  11. Host response biomarker in sepsis: suPAR detection.

    Science.gov (United States)

    Giamarellos-Bourboulis, Evangelos J; Georgitsi, Marianna

    2015-01-01

    Recent studies of our group have shown that suPAR may complement APACHE II score for risk assessment in sepsis. suPAR may be measured in serum of patients by an enzyme immunosorbent assay developed by Virogates (suPARnostic™). Production of suPAR from circulating neutrophils and monocytes may be assessed after isolation of neutrophils and monocytes and ex vivo culture. This is followed by measurement of suPAR in culture supernatants.

  12. Plant-derived SAC domain of PAR-4 (Prostate Apoptosis Response 4 exhibits growth inhibitory effects in prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Shayan eSarkar

    2015-10-01

    Full Text Available The gene Par-4 (Prostate Apoptosis Response 4 was originally identified in prostate cancer cells undergoing apoptosis and its product Par-4 showed cancer specific pro-apoptotic activity. Particularly, the SAC domain of Par-4 (SAC-Par-4 selectively kills cancer cells leaving normal cells unaffected. The therapeutic significance of bioactive SAC-Par-4 is enormous in cancer biology; however, its large scale production is still a matter of concern. Here we report the production of SAC-Par-4-GFP fusion protein coupled to translational enhancer sequence (5′ AMV and apoplast signal peptide (aTP in transgenic Nicotiana tabacum cv. Samsun NN plants under the control of a unique recombinant promoter M24. Transgene integration was confirmed by genomic DNA PCR, Southern and Northern blotting, Real-time PCR and Nuclear run-on assays. Results of Western blot analysis and ELISA confirmed expression of recombinant SAC-Par-4-GFP protein and it was as high as 0.15% of total soluble protein. In addition, we found that targeting of plant recombinant SAC-Par-4-GFP to the apoplast and endoplasmic reticulum (ER was essential for the stability of plant recombinant protein in comparison to the bacterial derived SAC-Par-4. Deglycosylation analysis demonstrated that ER-targeted SAC-Par-4-GFP-SEKDEL undergoes O-linked glycosylation unlike apoplast-targeted SAC-Par-4-GFP. Furthermore, various in vitro studies like mammalian cells proliferation assay (MTT, apoptosis induction assays, and NF-κB suppression suggested the cytotoxic and apoptotic properties of plant-derived SAC-Par-4-GFP against multiple prostate cancer cell lines. Additionally, pre-treatment of MAT-LyLu prostate cancer cells with purified SAC-Par-4-GFP significantly delayed the onset of tumor in a syngeneic rat prostate cancer model. Taken altogether, we proclaim that plant made SAC-Par-4 may become a useful alternate therapy for effectively alleviating cancer in the new era.

  13. La place de l'imagerie par résonance magnétique dans le carcinome lobulaire du sein

    Science.gov (United States)

    Bouzoubaa, Wail; Laadioui, Meryem; Alaoui, Fatime Zahra Fdili; Jayi, Sofia; Bouguern, Hakima; Chaara, Hikmat; Melhouf, Moulay Abdelilah

    2014-01-01

    Le carcinome lobulaire reste une entité histologique peu fréquente du cancer du sein, toute fois la place qu'occupe le cancer du sein actuellement dans la cancérologie féminine, justifie la connaissance des particularités de ce type de cancer mammaire. Le diagnostic paraclinique est basée sur le couple écho-mammographie a la recherche de multifocalité, multicentricité ou bilatéralité, d'où l'intérêt de l'IRM qui est la technique la plus sensible pour la mise en évidence de ces lésions et qui est devenue un examen de pratique courante dans le carcinome lobulaire du sein. Par le présent travail, et sous la lumière de la revue de la littérature, nous allons essayer de dégager les aspects épidémiologiques, cliniques, et paracliniques, du carcinome lobulaire du sein, et insister sur les indications et l'intérêt de l'IRM dans la prise en charge de ce type histologique. PMID:25368710

  14. Copenhagen uPAR prostate cancer (CuPCa) database

    DEFF Research Database (Denmark)

    Lippert, Solvej; Berg, Kasper D; Høyer-Hansen, Gunilla

    2016-01-01

    AIM: Urokinase plasminogen activator receptor (uPAR) plays a central role during cancer invasion by facilitating pericellular proteolysis. We initiated the prospective 'Copenhagen uPAR Prostate Cancer' study to investigate the significance of uPAR levels in prostate cancer (PCa) patients. METHODS...

  15. CD80, suPAR and nephrotic syndrome in a case of NPHS2 mutation.

    Science.gov (United States)

    Cara-Fuentes, Gabriel; Araya, Carlos; Wei, Changli; Rivard, Christopher; Ishimoto, Takuji; Reiser, Jochen; Johnson, Richard J; Garin, Eduardo H

    2013-01-01

    Podocin mutations are characterized by progression to end stage renal disease and histologic findings of Focal Segmental Glomerulosclerosis (FSGS). CD80 is a podocytes protein that may play a role in proteinuria, particularly in Minimal Change Disease whereas the soluble urokinase receptor (suPAR) is characteristically elevated in the serum of FSGS patients. In a patient with nephrotic syndrome and podocin mutation, urinary and serum CD80 as well as suPAR were measured using commercially available kits. Urinary CD80 molecular size was determined by western blot analysis. Glomerular staining for CD80 and podocin was performed. Patient displayed marked elevated CD80 and mildly increased suPAR urinary levels compared to controls. Serum CD80 level was within the range observed in normal controls. Serum suPAR level was elevated, albeit in the lower range reported for patients with primary FSGS. Immunofluorescence examination of kidney biopsy revealed glomerular CD80 expression. The combination of serum and urinary biomarkers can help differentiate various forms of FSGS. High urinary CD80 and elevated serum and urinary suPAR might represent a profile to differentiate this genetic form of FSGS from primary FSGS.

  16. Phosphorylation of Mycobacterium tuberculosis ParB participates in regulating the ParABS chromosome segregation system.

    Directory of Open Access Journals (Sweden)

    Grégory Baronian

    Full Text Available Here, we present for the first time that Mycobacterium tuberculosis ParB is phosphorylated by several mycobacterial Ser/Thr protein kinases in vitro. ParB and ParA are the key components of bacterial chromosome segregation apparatus. ParB is a cytosolic conserved protein that binds specifically to centromere-like DNA parS sequences and interacts with ParA, a weak ATPase required for its proper localization. Mass spectrometry identified the presence of ten phosphate groups, thus indicating that ParB is phosphorylated on eight threonines, Thr32, Thr41, Thr53, Thr110, Thr195, and Thr254, Thr300, Thr303 as well as on two serines, Ser5 and Ser239. The phosphorylation sites were further substituted either by alanine to prevent phosphorylation or aspartate to mimic constitutive phosphorylation. Electrophoretic mobility shift assays revealed a drastic inhibition of DNA-binding by ParB phosphomimetic mutant compared to wild type. In addition, bacterial two-hybrid experiments showed a loss of ParA-ParB interaction with the phosphomimetic mutant, indicating that phosphorylation is regulating the recruitment of the partitioning complex. Moreover, fluorescence microscopy experiments performed in the surrogate Mycobacterium smegmatis ΔparB strain revealed that in contrast to wild type Mtb ParB, which formed subpolar foci similar to M. smegmatis ParB, phoshomimetic Mtb ParB was delocalized. Thus, our findings highlight a novel regulatory role of the different isoforms of ParB representing a molecular switch in localization and functioning of partitioning protein in Mycobacterium tuberculosis.

  17. Phosphorylation of Mycobacterium tuberculosis ParB participates in regulating the ParABS chromosome segregation system.

    Science.gov (United States)

    Baronian, Grégory; Ginda, Katarzyna; Berry, Laurence; Cohen-Gonsaud, Martin; Zakrzewska-Czerwińska, Jolanta; Jakimowicz, Dagmara; Molle, Virginie

    2015-01-01

    Here, we present for the first time that Mycobacterium tuberculosis ParB is phosphorylated by several mycobacterial Ser/Thr protein kinases in vitro. ParB and ParA are the key components of bacterial chromosome segregation apparatus. ParB is a cytosolic conserved protein that binds specifically to centromere-like DNA parS sequences and interacts with ParA, a weak ATPase required for its proper localization. Mass spectrometry identified the presence of ten phosphate groups, thus indicating that ParB is phosphorylated on eight threonines, Thr32, Thr41, Thr53, Thr110, Thr195, and Thr254, Thr300, Thr303 as well as on two serines, Ser5 and Ser239. The phosphorylation sites were further substituted either by alanine to prevent phosphorylation or aspartate to mimic constitutive phosphorylation. Electrophoretic mobility shift assays revealed a drastic inhibition of DNA-binding by ParB phosphomimetic mutant compared to wild type. In addition, bacterial two-hybrid experiments showed a loss of ParA-ParB interaction with the phosphomimetic mutant, indicating that phosphorylation is regulating the recruitment of the partitioning complex. Moreover, fluorescence microscopy experiments performed in the surrogate Mycobacterium smegmatis ΔparB strain revealed that in contrast to wild type Mtb ParB, which formed subpolar foci similar to M. smegmatis ParB, phoshomimetic Mtb ParB was delocalized. Thus, our findings highlight a novel regulatory role of the different isoforms of ParB representing a molecular switch in localization and functioning of partitioning protein in Mycobacterium tuberculosis.

  18. Amendement 1 : Câbles électriques - Calcul du courant admissible - Partie 3: Sections concernant les conditions de fonctionnement - Section 2: Optimisation économique des sections d'âme de câbles électriques de puissance

    CERN Document Server

    International Electrotechnical Commission. Geneva

    1996-01-01

    Amendement 1 : Câbles électriques - Calcul du courant admissible - Partie 3: Sections concernant les conditions de fonctionnement - Section 2: Optimisation économique des sections d'âme de câbles électriques de puissance

  19. Une alternative au cobalt pour la synthese de nanotubes de carbone monoparoi par plasma inductif thermique

    Science.gov (United States)

    Carrier, Jean-Francois

    Les nanotubes de carbone de type monoparoi (C-SWNT) sont une classe recente de nanomateriaux qui ont fait leur apparition en 1991. L'interet qu'on leur accorde provient des nombreuses proprietes d'avant-plan qu'ils possedent. Leur resistance mecanique serait des plus rigide, tout comme ils peuvent conduire l'electricite et la chaleur d'une maniere inegalee. Non moins, les C-SWNT promettent de devenir une nouvelle classe de plateforme moleculaire, en servant de site d'attache pour des groupements reactifs. Les promesses de ce type particulier de nanomateriau sont nombreuses, la question aujourd'hui est de comment les realiser. La technologie de synthese par plasma inductif thermique se situe avantageusement pour la qualite de ses produits, sa productivite et les faibles couts d'operation. Par contre, des recherches recentes ont permis de mettre en lumiere des risques d'expositions reliees a l'utilisation du cobalt, comme catalyseur de synthese; son elimination ou bien son remplacement est devenu une preoccupation importante. Quatre recettes alternatives ont ete mises a l'essai afin de trouver une alternative plus securitaire a la recette de base; un melange catalytique ternaire, compose de nickel, de cobalt et d'oxyde d'yttrium. La premiere consiste essentiellement a remplacer la proportion massique de cobalt par du nickel, qui etait deja present dans la recette de base. Les trois options suivantes contiennent de nouveaux catalyseurs, en remplacement au Co, qui sont apparus dans plusieurs recherches scientifiques au courant des dernieres annees: le dioxyde de zircone (ZrO2), dioxyde de manganese (MnO2) et le molybdene (Mo). La methode utilisee consiste a vaporiser la matiere premiere, sous forme solide, dans un reacteur plasma a haute frequence (3 MHz) a paroi refroidi. Apres le passage dans le plasma, le systeme traverse une section dite de "croissance", isolee thermiquement a l'aide de graphite, afin de maintenir une certaine plage de temperature favorable a la

  20. Correlação da Presença de Flebotomíneos com Fatores Ambientais: Novos Parâmetros para Medidas de Prevenção e Controle das Leishmanioses | Correlation Between the Presence of Sandflies and Environmental Factors: New Parameters for the Control and Preventio

    Directory of Open Access Journals (Sweden)

    Baltazar Casagrande

    2014-05-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE Introdução: A Leishmaniose Tegumentar Americana (LTA e Leishmaniose Visceral Americana (LVA constituem um problema de saúde pública, assim como em parte dos continentes americano, asiático, europeu e africano. A importância das Leishmanioses levou a Organização Mundial de Saúde (OMS a incluí-las entre as seis doenças consideradas prioritárias em seu programa de controle. De acordo com os manuais do Ministério da Saúde, no ano de 2003 foram registrados casos de LTA em todos os estados brasileiros. No estado de São Paulo, no período de 1998 a 2011, foram notificados no total de 4.722 casos de LTA, autóctones de mais de 320 municípios. A LVA no Brasil, inicialmente tinha um caráter eminentemente rural e, mais recentemente, vem se expandindo para as áreas urbanas de médio e grande porte. Em 1999 foi registrado o primeiro caso humano de LVA em São Paulo e, desde então, o que vem ocorrendo é a difusão e a adaptação do vetor aos ecótopos urbanos, evento que se acredita ter iniciado em Araçatuba e vem se expandindo em direção ao oeste Paulista. Sabendo que os programas de prevenção e controle trabalhados pelo estado de São Paulo visam somente medidas de vigilância em torno dos casos de leishmanioses, o trabalho propõe apoiar estas medidas com novos parâmetros para prevenção e controle das Leishmanioses, tendo a pesquisa um recorte empírico o Pontal do Paranapanema, em especifico o Parque Estadual do Morro do Diabo situado no município de Teodoro Sampaio/SP. Metodologia/Desenvolvimento: Para realizar este trabalho deverá ser realizado o levantamento da fauna flebotomínica no PEMD. Serão definidos pontos de captura nas proximidades, nas bordas e no interior do referido parque. Para capturar os insetos vetores serão utilizadas armadilhas luminosas tipo CDC (Center on Disease Control, alimentadas com baterias 12 Volts cada, armadilha tipo Shannon, alimentada com

  1. Nouvelle structure de capteur à courants de Foucault et algorithme associé pour la mesure des propriétés électrique et magnétique d'un métal

    OpenAIRE

    Lê, Minh-Quang; Placko, Dominique

    1995-01-01

    Cet article décrit une nouvelle structure de capteur à courants de Foucault qui permet d'améliorer la sensibilité du capteur tout en conservant sa résolution latérale. Nous avons développé un modèle analytique de ce capteur qui peut être inversé pour estimer la conductivité électrique, la perméabilité magnétique d'une cible homogène et/ou la distance capteur-cible. La procédure d'inversion a été décrite et montre une possibilité d'estimer ces trois paramètres avec précision. L'expérience a ét...

  2. Eddy currents non-destructive testing. use of a numeric/symbolic method to separate and characterize the transitions of a signal; Diagnostic par courants de Foucault. Separation et caracterisation des transitoires d`un signal par une methode numerique/symbolique

    Energy Technology Data Exchange (ETDEWEB)

    Benas, J.C.; Lefevre, F.; Gaillard, P.; Georgel, B.

    1995-12-31

    This paper presents an original numeric/symbolic method for solving an inverse problem in the field of non-destructive testing. The purpose of this method is to characterize the transitions of a signal even when they are superimposed. Its principle is to solve as many direct problems as necessary to obtain the solution, and to use some hypothesis to manage the reasoning of the process. The direct problem calculation yields to a `model signal`, and the solution is reached when the model signal is close to the measured one. This method calculates the directions of minimization thanks to a symbolic reasoning based on the peaks of the residual signal. The results of the method are good and seem very promising. (authors). 13 refs., 13 figs., 5 tabs.

  3. Rassf5 and Ndr kinases regulate neuronal polarity through Par3 phosphorylation in a novel pathway.

    Science.gov (United States)

    Yang, Rui; Kong, Eryan; Jin, Jing; Hergovich, Alexander; Püschel, Andreas W

    2014-08-15

    The morphology and polarized growth of cells depend on pathways that control the asymmetric distribution of regulatory factors. The evolutionarily conserved Ndr kinases play important roles in cell polarity and morphogenesis in yeast and invertebrates but it is unclear whether they perform a similar function in mammalian cells. Here, we analyze the function of mammalian Ndr1 and Ndr2 (also known as STK38 or STK38L, respectively) in the establishment of polarity in neurons. We show that they act downstream of the tumor suppressor Rassf5 and upstream of the polarity protein Par3 (also known as PARD3). Rassf5 and Ndr1 or Ndr2 are required during the polarization of hippocampal neurons to prevent the formation of supernumerary axons. Mechanistically, the Ndr kinases act by phosphorylating Par3 at Ser383 to inhibit its interaction with dynein, thereby polarizing the distribution of Par3 and reinforcing axon specification. Our results identify a novel Rassf5-Ndr-Par3 signaling cascade that regulates the transport of Par3 during the establishment of neuronal polarity. Their role in neuronal polarity suggests that Ndr kinases perform a conserved function as regulators of cell polarity.

  4. Transcriptional program induced by factor VIIa-tissue factor, PAR1 and PAR2 in MDA-MB-231 cells

    DEFF Research Database (Denmark)

    Albrektsen, T; Sørensen, B B; Hjortø, G M

    2007-01-01

    -regulated genes was also regulated by a PAR1 agonist peptide suggesting extensive redundancy between FVIIa/PAR2 signaling and thrombin/PAR1 signaling. The FVIIa regulated genes encode cytokines, chemokines and growth factors, and the gene repertoire induced by FVIIa in MDA-MB-231 cells is consistent...... with a role for TF-FVIIa signaling in regulation of a wound healing type of response. Interestingly, a number of genes regulated exclusively by FVIIa/PAR2-mediated cell signaling in MDA-MB-231 cells were regulated by thrombin and a PAR1 agonist, but not by FVIIa, in the TF-expressing glioblastoma U373 cell...

  5. Pars plana vitrectomy for primary rhegmatogenous retinal detachment

    Directory of Open Access Journals (Sweden)

    Stephen G Schwartz

    2008-03-01

    Full Text Available Stephen G Schwartz, Harry W Flynn JrDepartment of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USAAbstract: Pars plana vitrectomy (PPV is growing in popularity for the treatment of primary rhegmatogenous retinal detachment (RD. PPV achieves favorable anatomic and visual outcomes in a wide variety of patients, especially in pseudophakic RD. A growing number of clinical series, both retrospective and prospective, have demonstrated generally comparable outcomes comparing PPV and scleral buckling (SB under a variety of circumstances. The Scleral Buckling Versus Primary Vitrectomy in Rhegmatogenous Retinal Detachment (SPR study is a multicenter, randomized, prospective, controlled clinical trial comparing SB versus PPV. This study should provide useful guidelines in the future. At this time, the choice of SB versus PPV should be based on the characteristics of the RD, the patient as a whole, and the experience and preference of the individual retinal surgeon.Keywords: pars plana vitrectomy, rhegmatogneous retinal detachment, scleral buckling

  6. Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells

    DEFF Research Database (Denmark)

    Hansen, Line; Unmack Larsen, Esben Kjær; Nielsen, Erik Holm

    2013-01-01

    Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted...... towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor...... to nanoparticles carrying a non-binding control peptide. In accordance with specific receptor-mediated recognition, a low uptake was observed in the presence of an excess of ATF, a natural ligand for uPAR. The uPAR specific magnetic nanoparticles can potentially provide a useful supplement for tumor patient...

  7. Path tracking control of mobile robots with techniques based on the use of curved abscissa; Suivi de trajectoire pour les robots mobiles par des techniques basees sur l`utilisation de l`abscisse curviligne

    Energy Technology Data Exchange (ETDEWEB)

    Micaelli, A.

    1992-10-01

    The paper describes a particular method, developed by the CEA, for the control of mobile robot trajectories based on Cornu`s spiral, i.e; sections of trajectories with constant curvature. New approaches are discussed for methods more convenient.

  8. The effect of one session of supragingival plaque control on clinical and biochemical parameters of chronic periodontitis Efeito de uma única sessão de controle de placa supragengival nos parâmetros clínicos e bioquímicos da periodontite crônica

    Directory of Open Access Journals (Sweden)

    Érica Del Peloso Ribeiro

    2005-09-01

    Full Text Available Supragingival plaque control is a requisite for the success of any periodontal procedure. However, little is know about the effect of this procedure alone on periodontitis. The aim of this study was to determine the effect of supragingival plaque control on clinical and biochemical parameters of chronic periodontitis. Twenty-five subjects exhibiting at least 4 pockets > 5mm, non-smokers and with no relevant systemic diseases, were selected for the study. The clinical and biochemical assessments were done before and 21 days after removal of plaque retentive factors, extraction of affected teeth and instruction in oral hygiene. The statistical analysis was done with the Student paired t-test (pO objetivo deste estudo foi avaliar o efeito do controle de placa supragengival sobre os parâmetros clínicos e bioquímicos da periodontite crônica. Foram selecionados 25 pacientes apresentando pelo menos 4 sítios com profundidade de sondagem > 5 mm, não fumantes e sem alterações sistêmicas relevantes. A avaliação clínica e bioquímica foi feita antes e 21 dias após a remoção dos fatores retentivos de placa, exodontia dos dentes condenados e instrução de higiene bucal. A análise estatística foi feita através do teste t de Student pareado (p<0,05 e demonstrou redução da porcentagem de sítios com sangramento à sondagem de 42,72 ± 15,83 para 35,87 ± 13,30 (p=0,002. Redução na profundidade de sondagem foi observada nos dentes anteriores (média inicial/final: 2,88 mm/2,78 mm; p=0,01 e faces interproximais (média inicial/final: 3,80 mm/3,65 mm; p=0,001. Houve aumento de 66,81% para 68,65% (p=0,03 no número de sítios com profundidade de sondagem de 1 a 3 mm e redução de 26,21% para 24,17% (p=0,04 nos de 4 a 6 mm. O valor do BAPNA apresentou redução de 51,44 ± 20,78 para 38,64 ± 12,34 (p=0,04. Baseado nesses achados pôde-se concluir que o controle de placa supragengival tem efeitos benéficos, principalmente relacionados à redu

  9. Study of the Plugging of Oil Well Sand Control Area in a Polymer Flooding Region Étude du colmatage aux abords des puits d’injection dans un réservoir d’hydrocarbure produit par balayage au polymère

    Directory of Open Access Journals (Sweden)

    Liu Dong

    2012-05-01

    Full Text Available The plugging of the oil well sand control area in a polymer flooding region was studied and the plugging substances were analyzed through UV-Vis spectrophotometer analysis, plasma emission spectrometry analysis, laser particle size analysis, X-Ray Diffractometer (XRD analysis, microscopic observation, chromatograph-mass spectrometer, etc. It is inferred that the main reason for the plugging of the sand control area was the adsorption, gathering and bridging of PAM on the surface of the sand. The migration of the mineral fines from the stratum and the adsorption of the crude oil ingredients on the surface of the fines also result in plugging. An experimental study on the polymer degradation was performed and two types of plug removal agents, potassium persulfate and sodium thiosulfate, were selected and tested. It showed that potassium persulfate and sodium thiosulfate could degrade the plugging substances and recover core permeability effectively. Moreover, field application tests were conducted in Gudao oil field which showed the restoration of the performance of various wells. It also indicated that the injection pressure obviously decreased and liquid production per day increased greatly after application of the plug removal agents in the sand control area. Le colmatage des zones de puits où un contrôle de venues de sable est réalisé par injection de polymère a été étudié et les substances à l’origine du colmatage ont été analysées par spectrophotométrie UV-Visible, spectrométrie à émission de plasma, analyse de taille de particules par diffraction laser, diffraction X (DRX, observation microscopique, spectrométrie de masse, etc. Nous avons conclu que la raison principale du colmatage de la zone de contrôle des venues de sable était l’adsorption, l’accumulation et le pontage du polyacrylamide (PAM à la surface du sable. La migration des fines du milieu poreux et l’adsorption de composés du brut à la surface des

  10. The actinobacterial signature protein ParJ (SCO1662) regulates ParA polymerization and affects chromosome segregation and cell division during Streptomyces sporulation.

    Science.gov (United States)

    Ditkowski, Bartosz; Troć, Paulina; Ginda, Katarzyna; Donczew, Magdalena; Chater, Keith F; Zakrzewska-Czerwińska, Jolanta; Jakimowicz, Dagmara

    2010-12-01

    Bacterial chromosome segregation usually involves cytoskeletal ParA proteins, ATPases which can form dynamic filaments. In aerial hyphae of the mycelial bacterium Streptomyces coelicolor, ParA filaments extend over tens of microns and are responsible for segregation of dozens of chromosomes. We have identified a novel interaction partner of S. coelicolor ParA, ParJ. ParJ negatively regulates ParA polymerization in vitro and is important for efficient chromosome segregation in sporulating aerial hyphae. ParJ-EGFP formed foci along aerial hyphae even in the absence of ParA. ParJ, which is encoded by sco1662, turned out to be one of the five actinobacterial signature proteins, and another of the five is a ParJ paralogue. We hypothesize that polar growth, which is characteristic not only of streptomycetes, but even of simple Actinobacteria, may be interlinked with ParA polymer assembly and its specific regulation by ParJ.

  11. A Conserved Mode of Protein Recognition and Binding in a ParD−ParE Toxin−Antitoxin Complex

    Energy Technology Data Exchange (ETDEWEB)

    Dalton, Kevin M.; Crosson, Sean (UC)

    2010-05-06

    Toxin-antitoxin (TA) systems form a ubiquitous class of prokaryotic proteins with functional roles in plasmid inheritance, environmental stress response, and cell development. ParDE family TA systems are broadly conserved on plasmids and bacterial chromosomes and have been well characterized as genetic elements that promote stable plasmid inheritance. We present a crystal structure of a chromosomally encoded ParD-ParE complex from Caulobacter crescentus at 2.6 {angstrom} resolution. This TA system forms an {alpha}{sub 2}{beta}{sub 2} heterotetramer in the crystal and in solution. The toxin-antitoxin binding interface reveals extensive polar and hydrophobic contacts of ParD antitoxin helices with a conserved recognition and binding groove on the ParE toxin. A cross-species comparison of this complex structure with related toxin structures identified an antitoxin recognition and binding subdomain that is conserved between distantly related members of the RelE/ParE toxin superfamily despite a low level of overall primary sequence identity. We further demonstrate that ParD antitoxin is dimeric, stably folded, and largely helical when not bound to ParE toxin. Thus, the paradigmatic model in which antitoxin undergoes a disorder-to-order transition upon toxin binding does not apply to this chromosomal ParD-ParE TA system.

  12. Parâmetros do controle postural em mulheres idosas com ou sem histórico de quedas associadas ou não à osteoartrite de joelhos Postural control parameters in elderly female fallers and non-fallers diagnosed or not with knee osteoarthritis

    Directory of Open Access Journals (Sweden)

    Marina Petrella

    2012-08-01

    Full Text Available OBJETIVOS: Comparar parâmetros estabilométricos de mulheres idosas com ou sem histórico de quedas associadas ou não à osteoartrite (OA de joelhos. MÉTODOS: Cinquenta e seis idosas apresentando ou não histórico de quedas (Q e OA de joelho unilateral e bilateral foram distribuídas da seguinte maneira: grupo QOA (n = 10, idosas com histórico de queda e OA de joelho; grupo QSOA (n = 11, idosas com histórico de queda e sem OA de joelho; grupo SQOA (n = 14, idosas sem histórico de quedas (SQ e com OA de joelho; e grupo SQSOA (n = 21, idosas sem histórico de quedas e sem OA de joelho. Para análise do equilíbrio semiestático usando uma plataforma de força, foram avaliados os deslocamentos anteroposterior (DAP e mediolateral (DML, as velocidades de oscilação anteroposterior (VAP e mediolateral (VML em quatro situações na postura ereta. As situações avaliadas foram as seguintes: 1 PFOA: sobre superfície fixa e olhos abertos; 2 PFOF: sobre superfície fixa e olhos fechados; 3 PIOA: sobre superfície instável e olhos abertos; 4 PIOF: sobre superfície instável e os olhos fechados. RESULTADOS: As idosas com OA de joelho apresentaram maior DAP em todas as situações analisadas (P 0,05. CONCLUSÕES: A OA de joelho, por si, é um fator prejudicial no aumento de oscilação do centro de pressão (COP na direção anteroposterior, enquanto o histórico de quedas, independente da presença de OA de joelhos, traz prejuízos ao controle postural na direção mediolateral.OBJECTIVES: To compare stabilometric parameters of elderly female fallers and non-fallers associated or not with knee osteoarthritis (OA. METHODS: Fifty-six elderly female fallers and non-fallers diagnosed or not with unilateral or bilateral knee OA were divided into the following groups: FOA (n = 10, elderly female fallers with knee OA; FNOA (n = 11, elderly female fallers without knee OA; NFOA (n = 14, elderly female non-fallers with knee OA; and NFNOA (n = 21, elderly

  13. A new model for estimating boreal forest fPAR

    Science.gov (United States)

    Majasalmi, Titta; Rautiainen, Miina; Stenberg, Pauline

    2014-05-01

    Life on Earth is continuously sustained by the extraterrestrial flux of photosynthetically active radiation (PAR, 400-700 nm) from the sun. This flux is converted to biomass by chloroplasts in green vegetation. Thus, the fraction of absorbed PAR (fPAR) is a key parameter used in carbon balance studies, and is listed as one of the Essential Climate Variables (ECV). Temporal courses of fPAR for boreal forests are difficult to measure, because of the complex 3D structures. Thus, they are most often estimated based on models which quantify the dependency of absorbed radiation on canopy structure. In this study, we adapted a physically-based canopy radiation model into a fPAR model, and compared modeled and measured fPAR in structurally different boreal forest stands. The model is based on the spectral invariants theory, and uses leaf area index (LAI), canopy gap fractions and spectra of foliage and understory as input data. The model differs from previously developed more detailed fPAR models in that the complex 3D structure of coniferous forests is described using an aggregated canopy parameter - photon recollision probability p. The strength of the model is that all model inputs are measurable or available through other simple models. First, the model was validated with measurements of instantaneous fPAR obtained with the TRAC instrument in nine Scots pine, Norway spruce and Silver birch stands in a boreal forest in southern Finland. Good agreement was found between modeled and measured fPAR. Next, we applied the model to predict temporal courses of fPAR using data on incoming radiation from a nearby flux tower and sky irradiance models. Application of the model to simulate diurnal and seasonal values of fPAR indicated that the ratio of direct-to-total incident radiation and leaf area index are the key factors behind the magnitude and variation of stand-level fPAR values.

  14. Control del parásito Varroa destructor (Acari: Varroidae en colmenas de la abeja Apis mellifera (Hymenoptera: Apidae mediante la aplicación de la técnica de entrampado

    Directory of Open Access Journals (Sweden)

    Natalia DAMIANI

    2006-01-01

    Full Text Available La parasitosis causada por el ácaro Varroa destructor (Anderson & Trueman es, actualmente considerada el mayor escollo para el desarrollo de la apicultura. El objetivo del presente trabajo fue evaluar la técnica del entrampamiento de ácaros en panales de cría, como posible método de control de la parasitosis. El trabajo se llevó a cabo en Coronel Vidal, provincia de Buenos Aires. Se trabajó sobre colmenas tipo Langstroth del híbrido regional de Apis mellifera (Linneaus. En cada colmena experimental se procedió a confinar a la reina en panales trampa específicos, con el fin de poder controlar la oviposición. Estos panales, luego de ser operculados por las obreras, fueron llevados al laboratorio donde se desoperculó cada una de las celdas de cría, y se contabilizó el número de ácaros presentes. Esta técnica se aplicó variando el número de panales trampa (1-3 colocados, tanto para los constituidos por celdas de cría de obreras como de zánganos. También, se evaluó el impacto de la aplicación de esta técnica sobre el desarrollo de las colonias, mediante la medición de su productividad. Los resultados indican, que la técnica empleada sólo es efectiva, cuando se aplican tres panales de cría de zánganos de manera consecutiva alcanzando una efectividad máxima de 84%. Cuando se aplican tres panales de obreras, la técnica mostró niveles de efectividad muy inferiores (14%. En las colonias sobre las que se aplicó esta técnica, la productividad de miel se redujo significativamente, comparada con las colonias control. Esta técnica resulta ideal para ser combinada con otros mecanismos de control, disminuyendo la aplicación de sustancias químicas que puedan contaminar la miel, y la generación de resistencia por parte del ácaro frente a los principios activos utilizados para su control.

  15. Transcriptional program induced by factor VIIa-tissue factor, PAR1 and PAR2 in MDA-MB-231 cells

    DEFF Research Database (Denmark)

    Albrektsen, Tatjana; Sørensen, B B; Hjortø, G M

    2007-01-01

    -activated receptor 1 (PAR1) or PAR2 agonists using MDA-MB-231 breast carcinoma cells that constitutively express TF, PAR1 and PAR2. RESULTS AND CONCLUSIONS: Out of 8500 genes, FVIIa stimulation induced differential regulation of 39 genes most of which were not previously recognized as FVIIa regulated. All genes...... regulated genes encode cytokines, chemokines and growth factors, and the gene repertoire induced by FVIIa in MDA-MB-231 cells is consistent with a role for TF-FVIIa signaling in regulation of a wound healing type of response. Interestingly, a number of genes regulated exclusively by FVIIa/PAR2-mediated cell...... signaling in MDA-MB-231 cells were regulated by thrombin and a PAR1 agonist, but not by FVIIa, in the TF-expressing glioblastoma U373 cell line....

  16. Bancroftian filariasis in Belém, Pará State: possibilities for eradication by introducing modern methods of control for Culex quinquefasciatus A filariose bancroftiana em Belém, Pará: possibilidades atuais de erradicação mediante a integração à campanha de novos métodos de controle da população de Culex quinquefasciatus

    Directory of Open Access Journals (Sweden)

    Habib Fraiha Neto

    1993-12-01

    Full Text Available For the past four decades, Belém has been considered to be the largest focus of Bancroftian filariasis in the Amazon region and one of the most important ones in Brazil. In 1952, practically one-fifth of the population was infected and it was estimated that there were 2,500 cases of Bancroftian elephantiasis. The major factor considered to be responsible for this situation was the high density of the main vector species: it was calculated that in April 1943 there was a mean of 67 infected mosquitoes per house. A control campaign was initiated in 1952, involving treatment of patients with diethylcarbamazine, as well as control of the vector. In 1956, vector control was discontinued because of a lack of efficient insecticides and financial limitations. Even so, the campaign was very successful and the incidence was reduced from 19.9% to 0.03%. Total eradication could be achieved if new methods of vector control were introduced, such as the use of Reiter's expanded polystyrene balls in cesspits and pit latrines, biological control using pathogenic bacteria, and improvement of basic sanitation.Há mais de quatro décadas Belém é considerada o maior foco de bancroftose da região amazônica e um dos mais importantes do Brasil. Em 1952, praticamente um quinto de sua população apresentava microfilárias no sangue, estimando-se em 2.500 os casos de elefantíase. O fator considerado o maior condicionante de tão extraordinária freqüência era a alta densidade local do principal vetor, Culex quinquefasciatus. Em abril de 1943 calculava-se uma média de 67 fêmeas da espécie infectadas por domicílio. Uma campanha de controle foi deflagrada em 1952, incluindo busca ativa e tratamento dos casos com dietilcarbamazina, além medidas de combate ao vetor. Estas foram abandonadas em 1956, por força da ineficácia ou do alto custo dos inseticidas. Ainda assim, a campanha foi muito bem sucedida, reduzindo a incidência de 19,9 para 0,03%. Hoje, a erradica

  17. Differential uPAR recruitment in caveolar-lipid rafts by GM1 and GM3 gangliosides regulates endothelial progenitor cells angiogenesis

    Science.gov (United States)

    Margheri, Francesca; Papucci, Laura; Schiavone, Nicola; D'Agostino, Riccardo; Trigari, Silvana; Serratì, Simona; Laurenzana, Anna; Biagioni, Alessio; Luciani, Cristina; Chillà, Anastasia; Andreucci, Elena; Del Rosso, Tommaso; Margheri, Giancarlo; Del Rosso, Mario; Fibbi, Gabriella

    2015-01-01

    Gangliosides and the urokinase plasminogen activator receptor (uPAR) tipically partition in specialized membrane microdomains called lipid-rafts. uPAR becomes functionally important in fostering angiogenesis in endothelial progenitor cells (EPCs) upon recruitment in caveolar-lipid rafts. Moreover, cell membrane enrichment with exogenous GM1 ganglioside is pro-angiogenic and opposite to the activity of GM3 ganglioside. On these basis, we first checked the interaction of uPAR with membrane models enriched with GM1 or GM3, relying on the adoption of solid-supported mobile bilayer lipid membranes with raft-like composition formed onto solid hydrophilic surfaces, and evaluated by surface plasmon resonance (SPR) the extent of uPAR recruitment. We estimated the apparent dissociation constants of uPAR-GM1/GM3 complexes. These preliminary observations, indicating that uPAR binds preferentially to GM1-enriched biomimetic membranes, were validated by identifying a pro-angiogenic activity of GM1-enriched EPCs, based on GM1-dependent uPAR recruitment in caveolar rafts. We have observed that addition of GM1 to EPCs culture medium promotes matrigel invasion and capillary morphogenesis, as opposed to the anti-angiogenesis activity of GM3. Moreover, GM1 also stimulates MAPKinases signalling pathways, typically associated with an angiogenesis program. Caveolar-raft isolation and Western blotting of uPAR showed that GM1 promotes caveolar-raft partitioning of uPAR, as opposed to control and GM3-challenged EPCs. By confocal microscopy, we have shown that in EPCs uPAR is present on the surface in at least three compartments, respectively, associated to GM1, GM3 and caveolar rafts. Following GM1 exogenous addition, the GM3 compartment is depleted of uPAR which is recruited within caveolar rafts thereby triggering angiogenesis. PMID:25313007

  18. Differential uPAR recruitment in caveolar-lipid rafts by GM1 and GM3 gangliosides regulates endothelial progenitor cells angiogenesis.

    Science.gov (United States)

    Margheri, Francesca; Papucci, Laura; Schiavone, Nicola; D'Agostino, Riccardo; Trigari, Silvana; Serratì, Simona; Laurenzana, Anna; Biagioni, Alessio; Luciani, Cristina; Chillà, Anastasia; Andreucci, Elena; Del Rosso, Tommaso; Margheri, Giancarlo; Del Rosso, Mario; Fibbi, Gabriella

    2015-01-01

    Gangliosides and the urokinase plasminogen activator receptor (uPAR) tipically partition in specialized membrane microdomains called lipid-rafts. uPAR becomes functionally important in fostering angiogenesis in endothelial progenitor cells (EPCs) upon recruitment in caveolar-lipid rafts. Moreover, cell membrane enrichment with exogenous GM1 ganglioside is pro-angiogenic and opposite to the activity of GM3 ganglioside. On these basis, we first checked the interaction of uPAR with membrane models enriched with GM1 or GM3, relying on the adoption of solid-supported mobile bilayer lipid membranes with raft-like composition formed onto solid hydrophilic surfaces, and evaluated by surface plasmon resonance (SPR) the extent of uPAR recruitment. We estimated the apparent dissociation constants of uPAR-GM1/GM3 complexes. These preliminary observations, indicating that uPAR binds preferentially to GM1-enriched biomimetic membranes, were validated by identifying a pro-angiogenic activity of GM1-enriched EPCs, based on GM1-dependent uPAR recruitment in caveolar rafts. We have observed that addition of GM1 to EPCs culture medium promotes matrigel invasion and capillary morphogenesis, as opposed to the anti-angiogenesis activity of GM3. Moreover, GM1 also stimulates MAPKinases signalling pathways, typically associated with an angiogenesis program. Caveolar-raft isolation and Western blotting of uPAR showed that GM1 promotes caveolar-raft partitioning of uPAR, as opposed to control and GM3-challenged EPCs. By confocal microscopy, we have shown that in EPCs uPAR is present on the surface in at least three compartments, respectively, associated to GM1, GM3 and caveolar rafts. Following GM1 exogenous addition, the GM3 compartment is depleted of uPAR which is recruited within caveolar rafts thereby triggering angiogenesis.

  19. CFD Simulation of Hydrodynamic and Thermal Performance of a Micro Heat Exchanger Simulation numérique par CFD de l’hydrodynamique et des performances thermiques d’un micro échangeur de chaleur

    Directory of Open Access Journals (Sweden)

    Nastoll W.

    2010-10-01

    Full Text Available To evaluate the performances and limitations of compact heat exchanger with micro-structured plates, CFD simulations of hydrodynamics and thermal transfers have been performed inside a commercial micro heat exchanger from IMM operated in liquid/liquid flows. The hydrodynamic results show that the flow rate distribution over the plates is rather homogeneous with some velocity gradient at the channel inlet due to inertial effect in the distributor. Fluid temperature profiles are both influenced by convective transfer in the channels and in the distributing/collecting sections and by conductive transfer through the metal wall at the plate periphery especially at low flow rates. Due to undesired heat transfer by conduction, the fraction of heat power really transferred inside the channels varies from 75 to 85% in counter current flow configuration and between 35 and 70% in co-current flow configuration. Computational results are successfully compared to 2D experimental temperature profiles measured inside the heat exchanger. Pour évaluer les performances et les limitations d’un échangeur thermique compact composé de plaques micro-structurées, des simulations CFD de l’hydrodynamique et des transferts thermiques ont été effectués en écoulement liquide/liquide pour un micro-échangeur commercialisé par l’IMM. Les résultats de la simulation hydrodynamique montrent que la distribution des flux de l’écoulement liquide est plutôt homogène avec quelques gradients de vitesses à l’entrée des canaux à cause d’effets inertiels dans le distributeur. Les profils de température sont influencés à la fois par les transferts thermiques par convection dans les canaux et dans les zones de distribution et de collecte de l’écoulement et également par les transferts par conduction aux travers des parois métalliques situées entre les canaux et à la périphérie de la zone micro-structurée, en particulier pour les faibles d

  20. Análisis de un programa de pérdida de peso con sustitutivos de comidas sobre el control del peso y de parámetros bioquímicos en pacientes con sobrepeso y obesidad grado I Analysis of a weight loss program with meal replacement products on weight and biochemical markers in overweight or type I obese patients

    Directory of Open Access Journals (Sweden)

    J. Basulto

    2008-08-01

    Full Text Available Introducción: Existen pocos estudios que hayan evaluado la eficacia y la seguridad de los sustitutivos de comidas para perder peso. Objetivo: Evaluar la eficacia y la seguridad de un programa de pérdida de peso que incluía productos sustitutivos de comidas. Métodos: Se evaluó el efecto de una dieta hipocalórica versus una dieta hipocalórica que incluía sustitutivos de comidas en 55 voluntarios con sobrepeso u obesidad grado I. Los sujetos fueron distribuidos en un grupo control (dieta hipocalórica o un grupo de intervención (dieta hipocalórica con sustitutivos de comidas durante 8 semanas. Se registraron datos antropométricos, hábito tabáquico y nivel de actividad física. También se realizaron extracciones sanguíneas para evaluar cambios bioquímicos al inicio del estudio, a las 4 semanas y al final del estudio. Resultados: El grupo control perdió 3,97 kg de media, mientras que en el grupo intervención se observó una pérdida de 4,44 kg, no siendo estas diferencias estadísticamente significativas entre grupos. Otros parámetros antropométricos como el perímetro de la cintura y el perímetro de la cadera también disminuyeron en ambos grupos, aunque sin diferencias entre grupos. Se observó una disminución estadísticamente significativa (p = 0,041 en los valores de triglicéridos, aunque también sin diferencias entre grupos. Discusión: Los sustitutivos de comidas, dentro de un programa dietético controlado, fueron tan eficaces y seguros para perder peso y modificar otros parámetros antropométricos como el tratamiento dietético convencional sin sustitutivos.Background: There are scarce data about the efficacy and security of meal replacement products as a strategy to weight loss. Aim: To evaluate the efficacy and safety of a weight loss program that includes meal replacement products. Methods: We evaluated the effect of a hypocaloric diet versus a hypocaloric diet that includes meal replacement products in 55

  1. Taare Zameen Par and dyslexic savants

    Science.gov (United States)

    Chakravarty, Ambar

    2009-01-01

    The film Taare Zameen Par (Stars upon the Ground) portrays the tormented life at school and at home of a child with dyslexia and his eventual success after his artistic talents are discovered by his art teacher at the boarding school. The film hints at a curious neurocognitive phenomenon of creativity in the midst of language disability, as exemplified in the lives of people like Leonardo da Vinci and Albert Einstein, both of whom demonstrated extraordinary creativity even though they were probably affected with developmental learning disorders. It has been hypothesized that a developmental delay in the dominant hemisphere most likely ‘disinhibits’ the nondominant parietal lobe, unmasking talents—artistic or otherwise—in some such individuals. It has been suggested that, in remedial training, children with learning disorders be encouraged to develop such hidden talents to full capacity, rather than be subjected to the usual overemphasis on the correction of the disturbed coded symbol operations. PMID:20142854

  2. Parálisis cerebral Cerebral palsy

    OpenAIRE

    Jorge Malagon Valdez

    2007-01-01

    El término parálisis cerebral (PC) engloba a un gran número de síndromes neurológicos clínicos, de etiología diversa. Estos síndromes se caracterizan por tener una sintomatología común: los trastornos motores. Algunos autores prefieren manejar términos como "encefalopatía fija", "encefalopatías no evolutivas". Se mencionan la utilidad de programas de intervención temprana y métodos especiales de rehabilitación, así como el manejo de las deficiencias asociadas como la epilepsia, deficiencia me...

  3. Taare Zameen Par and dyslexic savants

    Directory of Open Access Journals (Sweden)

    Chakravarty Ambar

    2009-01-01

    Full Text Available The film Taare Zameen Par (Stars upon the Ground portrays the tormented life at school and at home of a child with dyslexia and his eventual success after his artistic talents are discovered by his art teacher at the boarding school. The film hints at a curious neurocognitive phenomenon of creativity in the midst of language disability, as exemplified in the lives of people like Leonardo da Vinci and Albert Einstein, both of whom demonstrated extraordinary creativity even though they were probably affected with developmental learning disorders. It has been hypothesized that a developmental delay in the dominant hemisphere most likely ′disinhibits′ the nondominant parietal lobe, unmasking talents-artistic or otherwise-in some such individuals. It has been suggested that, in remedial training, children with learning disorders be encouraged to develop such hidden talents to full capacity, rather than be subjected to the usual overemphasis on the correction of the disturbed coded symbol operations.

  4. Estimação de parâmetros genéticos para produção de leite no dia do controle e produção acumulada até 305 dias, para as primeiras lactações de vacas da raça Caracu

    Directory of Open Access Journals (Sweden)

    El Faro Lenira

    2003-01-01

    Full Text Available Foram estimados parâmetros genéticos para produção de leite no dia do controle e produção acumulada até 305 dias (P305 de primeiras lactações de vacas da raça Caracu. O modelo animal considerado conteve efeito genético aditivo, como aleatório, além dos efeitos fixos de grupo contemporâneo e da idade ao parto, como covariável. Foram definidos dois grupos contemporâneos para explicar a variação ocorrida nas produções em cada controle leiteiro, compostos por ano, semana do controle e retiro (gc1 ou ano, mês do controle e retiro (gc2. Os componentes de variância foram estimados pelo método da máxima verossimilhança restrita. As variâncias fenotípicas, residuais e genéticas foram maiores no início da lactação, entretanto, a variância genética aditiva foi proporcionalmente menor em relação às demais variâncias. As estimativas de herdabilidade oscilaram entre 0,09 e 0,32 e foram maiores no final da lactação, indicando maior variabilidade genética nesse período. As correlações genéticas (r a entre as produções em cada controle foram positivas e maiores, quanto menor a distância entre eles. A herdabilidade para P305 foi de 0,27 e as r a desta com os controles, positivas e elevadas, principalmente no meio da lactação. Os resultados indicam que a seleção direta para P305 proporciona maiores ganhos genéticos para essa característica que a obtida por meio de resposta correlacionada para as produções em cada controle.

  5. The reprocessing of irradiated fuels by halides and their compounds; Le traitement des combustibles irradies par les halogenes et leurs composes

    Energy Technology Data Exchange (ETDEWEB)

    Bourgeois, M.; Faugeras, P. [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

    1964-07-01

    A brief description is given of the experiments leading to the choice of the process volatilization of fluorides by gas phase attack. The chemical process is described for certain current types of clad Fuels: the aluminium or the zirconium cladding is first volatilized as chloride by attack with gaseous hydrogen chloride. The uranium is then transformed into volatile hexafluoride by attack with fluorine. These reactions are carried out consecutively in the same reactor in the presence of a fluidized bed of alumina which facilitates heat exchange. The experiments have been carried out in quantities from 100 gms to several kilograms of fuel, first without activity, and then with tracers. A description is given of the laboratory research which was carried out simultaneously on the separation of uranium and plutonium fluorides. Finally, an apparatus is described which is intended to test the process on irradiated fuel at an activity level of several thousands of curies of fission products. (authors) [French] On rappelle brievement les experimentations qui nous ont permis de decider du procede adopte volatilisation des fluorures par attaque en phase gazeuse. On decrit le processus chimique pour certains types courants de combustibles Gaines: dans un premier stade, l'aluminium ou le zirconium est volatilise sous forme de chlorure par action de l'acide chlorhydrique. Ensuite, l'uranium est transforme en hexafluorure volatil par action du fluor. Ces operations se font successivement dans un meme reacteur, en presence d'un lit fluidise d'alumine qui a pour but de faciliter les echanges thermiques. L'experimentation a ete conduite sur des quantites allant de 100 g a plusieurs kg de combustibles, en inactif, puis avec des traceurs. On decrit les etudes de laboratoire menees parallelement sur la separation des fluorures d'uranium et de plutonium. Enfin, on decrit une installation en construction destinee a experimenter le procede sur

  6. Mechanism of DNA Segregation in Prokaryotes: Replicon Pairing by parC of Plasmid R1

    Science.gov (United States)

    Jensen, Rasmus Bugge; Lurz, Rudi; Gerdes, Kenn

    1998-07-01

    Prokaryotic chromosomes and plasmids encode partitioning systems that are required for DNA segregation at cell division. The systems are thought to be functionally analogous to eukaryotic centromeres and to play a general role in DNA segregation. The parA system of plasmid R1 encodes two proteins ParM and ParR, and a cis-acting centromere-like site denoted parC. The ParR protein binds to parC in vivo and in vitro. The ParM protein is an ATPase that interacts with ParR specifically bound to parC. Using electron microscopy, we show here that parC mediates efficient pairing of plasmid molecules. The pairing requires binding of ParR to parC and is stimulated by the ParM ATPase. The ParM mediated stimulation of plasmid pairing is dependent on ATP hydrolysis by ParM. Using a ligation kinetics assay, we find that ParR stimulates ligation of parC-containing DNA fragments. The rate-of-ligation was increased by wild type ParM protein but not by mutant ParM protein deficient in the ATPase activity. Thus, two independent assays show that parC mediates pairing of plasmid molecules in vitro. These results are consistent with the proposal that replicon pairing is part of the mechanism of DNA segregation in prokaryotes.

  7. Regulatory cross-talk in the double par locus of plasmid pB171

    DEFF Research Database (Denmark)

    Ringgaard, Simon; Ebersbach, Gitte; Borch, Jonas;

    2007-01-01

    partition and promoter repression complexes. Surprisingly, ParB repressed transcription of the noncognate par operon, indicating cross-talk and possibly epistasis between the two systems. The par promoters, P1 and P2, affected each other negatively. The DNA binding activities of ParR and ParB correlated...

  8. Bacterial mitosis: partitioning protein ParA oscillates in spiral-shaped structures and positions plasmids at mid-cell

    DEFF Research Database (Denmark)

    Ebersbach, Gitte; Gerdes, Kenn; Charbon, Gitte Ebersbach

    2004-01-01

    The par2 locus of Escherichia coli plasmid pB171 encodes oscillating ATPase ParA, DNA binding protein ParB and two cis-acting DNA regions to which ParB binds (parC1 and parC2). Three independent techniques were used to investigate the subcellular localization of plasmids carrying par2. In cells w...

  9. ParABS system in chromosome partitioning in the bacterium Myxococcus xanthus.

    Directory of Open Access Journals (Sweden)

    Antonio A Iniesta

    Full Text Available Chromosome segregation is an essential cellular function in eukaryotic and prokaryotic cells. The ParABS system is a fundamental player for a mitosis-like process in chromosome partitioning in many bacterial species. This work shows that the social bacterium Myxococcus xanthus also uses the ParABS system for chromosome segregation. Its large prokaryotic genome of 9.1 Mb contains 22 parS sequences near the origin of replication, and it is shown here that M. xanthus ParB binds preferentially to a consensus parS sequence in vitro. ParB and ParA are essential for cell viability in M. xanthus as in Caulobacter crescentus, but unlike in many other bacteria. Absence of ParB results in anucleate cells, chromosome segregation defects and loss of viability. Analysis of ParA subcellular localization shows that it clusters at the poles in all cells, and in some, in the DNA-free cell division plane between two chromosomal DNA masses. This ParA localization pattern depends on ParB but not on FtsZ. ParB inhibits the nonspecific interaction of ParA with DNA, and ParA colocalizes with chromosomal DNA only when ParB is depleted. The subcellular localization of ParB suggests a single ParB-parS complex localized at the edge of the nucleoid, next to a polar ParA cluster, with a second ParB-parS complex migrating after the replication of parS takes place to the opposite nucleoid edge, next to the other polar ParA cluster.

  10. ParABS system in chromosome partitioning in the bacterium Myxococcus xanthus.

    Science.gov (United States)

    Iniesta, Antonio A

    2014-01-01

    Chromosome segregation is an essential cellular function in eukaryotic and prokaryotic cells. The ParABS system is a fundamental player for a mitosis-like process in chromosome partitioning in many bacterial species. This work shows that the social bacterium Myxococcus xanthus also uses the ParABS system for chromosome segregation. Its large prokaryotic genome of 9.1 Mb contains 22 parS sequences near the origin of replication, and it is shown here that M. xanthus ParB binds preferentially to a consensus parS sequence in vitro. ParB and ParA are essential for cell viability in M. xanthus as in Caulobacter crescentus, but unlike in many other bacteria. Absence of ParB results in anucleate cells, chromosome segregation defects and loss of viability. Analysis of ParA subcellular localization shows that it clusters at the poles in all cells, and in some, in the DNA-free cell division plane between two chromosomal DNA masses. This ParA localization pattern depends on ParB but not on FtsZ. ParB inhibits the nonspecific interaction of ParA with DNA, and ParA colocalizes with chromosomal DNA only when ParB is depleted. The subcellular localization of ParB suggests a single ParB-parS complex localized at the edge of the nucleoid, next to a polar ParA cluster, with a second ParB-parS complex migrating after the replication of parS takes place to the opposite nucleoid edge, next to the other polar ParA cluster.

  11. The photothermal camera - a new non destructive inspection tool; La camera photothermique - une nouvelle methode de controle non destructif

    Energy Technology Data Exchange (ETDEWEB)

    Piriou, M. [AREVA NP Centre Technique SFE - Zone Industrielle et Portuaire Sud - BP13 - 71380 Saint Marcel (France)

    2007-07-01

    techniques classiques de controle de surface (ressuage, magnetoscopie, courants de Foucault) pour: - detecter sans aucun contact, des defauts sous ligaments ou debouchants de quelques microns d'ouverture, sur des pieces metalliques non preparees (oxydees, usinees, soudees), - fonctionner sur des surfaces aux geometries variees, sur des pieces chaudes, sur des materiaux isolants (dielectriques), sans etre affectee par les proprietes magnetiques de la piece a examiner. Cette methode a permis, entre autre, de controler in situ les soudures 'tube/plaque' d'un echangeur intermediaire du reacteur rapide Phenix, de prouver qu'elle est une alternative au ressuage pour le controle des soudures de l'enceinte a vide d'ITER, de detecter des fissures dans les soudures (ex: J-weld d'adaptateurs de couvercles) et de reveler la fissuration amorcee par faiencage thermique. Les particularites de cette methode innovante sont: - de fonctionner a distance de la piece a controler, jusqu'a 2 metres, - d'etre totalement telecommandee a la distance de 15 metres (voire beaucoup plus par lien Ethernet), - d'etre une methode 'propre' puisqu'elle ne genere aucun dechet. Ces particularites en font une methode alternative au ressuage, afin de garantir la protection des operateurs et de l'environnement. (auteur)

  12. Plasma suPAR is lowered by smoking cessation

    DEFF Research Database (Denmark)

    Eugen-Olsen, Jesper; Ladelund, Steen; Sørensen, Lars Tue

    2016-01-01

    in 46 never smokers (1·9 ng/mL (1·7-2·2)). In smokers randomized to smoking cessation, suPAR levels after 4 weeks of stopping were decreased and no longer significantly different from the never smokers values. SuPAR decreased in both those who received a placebo as well as nicotine patch. Interestingly......, those with the highest suPAR level at time of smoking were also those with the highest level of suPAR after smoking cessation. In contrast, smoking or smoking cessation had no influence on CRP levels. CONCLUSION: Our study suggests that the suPAR level may aid to personalize the risk of smoking...... by identifying those smokers with the highest risk of developing disease and who may have the most benefit of smoking cessation....

  13. [Around Ambroise Paré: his pupils and friends].

    Science.gov (United States)

    Dumaître, P

    1996-01-01

    The most important pupil of Paré was Jacques Guillemeau (1550-1613), a famous surgeon from Montpellier. He lived at Paré's during eight years and wrote there his first work "Traité des maladies des yeux" (1585) and was really his "spiritual son". The barber-surgeon Martin Boursier, husband of the famous midwife Louise Bourgeois stayed twenty years with Paré and she learned her practice in his works. Attracted by Paré's fame, Melchior Sebiz (1539-1625) who shall become a famous professor of medicine in Strasbourg attended Paré's lessons and "was with him in great friendship". Among his friends, Thierry de Héry (ca. 1505-ca. 1560), companion of his youth as a barber-surgeon and author of the first French book on syphilis seems to have been the dearest and the nearest to his heart.

  14. The urokinase receptor (uPAR) and the uPAR-associated protein (uPARAP/Endo180)

    DEFF Research Database (Denmark)

    Behrendt, Niels

    2004-01-01

    processes involve a highly organized interplay between proteases and their cellular binding sites as well as specific substrates and internalization receptors. This review article is focused on two components, the urokinase plasminogen activator receptor (uPAR) and the uPAR-associated protein (uPARAP, also...

  15. Effects of thrombin, PAR-1 activating peptide and a PAR-1 antagonist on umbilical artery resistance in vitro

    Directory of Open Access Journals (Sweden)

    Elliott John T

    2005-02-01

    Full Text Available Abstract Background The non-thrombotic effects of thrombin in cardiovascular tissues, as mediated via the protease activated receptors (PARs, and particularly PAR-1, have been the focus of much recent research. The aims of this study were to evaluate the effects of thrombin, a specific PAR-1 activating peptide (PAR1-AP, and a PAR-1 antagonist on human umbilical artery tone in vitro. Methods Human umbilical artery samples were obtained from 17 women at term. Arterial rings were suspended under physiologic conditions for isometric recording. The in vitro effects of thrombin (0.5 units/mL to 3 units/mL, PAR1-AP TFLLR-NH2 [10(-9 to 10(-6 M], and PAR-1 antagonist (N-trans cinnamoyl- p-fluoroPhe-p-guanidinoPhe-Leu-Arg-Orn-NH2 [10(-9 M to 10(-5 M] on umbilical artery tone were measured. Results Both thrombin and TFLLR-NH2 exerted a potent cumulative vasodilatory effect on human umbilical artery resistance (P 0.05. Conclusion These findings highlight a potential role for thrombin and PAR-1 receptors in vascular regulation of feto-placental blood flow in normal pregnancy, and in association with the vascular lesions associated with IUGR and pre-eclampsia.

  16. General Yang-Mills type gauge theories for p-form gauge fields: From physics-based ideas to a mathematical framework or From Bianchi identities to twisted Courant algebroids

    Science.gov (United States)

    Grützmann, Melchior; Strobl, Thomas

    2015-10-01

    Starting with minimal requirements from the physical experience with higher gauge theories, i.e. gauge theories for a tower of differential forms of different form degrees, we discover that all the structural identities governing such theories can be concisely recombined into what is called a Q-structure or, equivalently, an L∞-algebroid. This has many technical and conceptual advantages: complicated higher bundles become just bundles in the category of Q-manifolds in this approach (the many structural identities being encoded in the one operator Q squaring to zero), gauge transformations are generated by internal vertical automorphisms in these bundles and even for a relatively intricate field content the gauge algebra can be determined in some lines and is given by what is called the derived bracket construction. This paper aims equally at mathematicians and theoretical physicists; each more physical section is followed by a purely mathematical one. While the considerations are valid for arbitrary highest form degree p, we pay particular attention to p = 2, i.e. 1- and 2-form gauge fields coupled nonlinearly to scalar fields (0-form fields). The structural identities of the coupled system correspond to a Lie 2-algebroid in this case and we provide different axiomatic descriptions of those, inspired by the application, including e.g. one as a particular kind of a vector-bundle twisted Courant algebroid.

  17. Nouvelle structure de capteur à courants de Foucault et algorithme associé pour la mesure des propriétés électrique et magnétique d'un métal

    Science.gov (United States)

    Lê, Minh-Quang; Placko, Dominique

    1995-06-01

    This paper describes a new structure for eddy-current transducers which improves sensitivity while maintaining high lateral resolution. We have developed an analytical model to allow a model-based inversion from transducers signals to estimate thick and homogeneous plates electrical conductivity, magnetic permeability and/or lift-off. The inversion procedure is described and shows the possibility of a precise simultaneous estimation of the three parameters. Experiments were conducted with magnetic and non magnetic metals. Cet article décrit une nouvelle structure de capteur à courants de Foucault qui permet d'améliorer la sensibilité du capteur tout en conservant sa résolution latérale. Nous avons développé un modèle analytique de ce capteur qui peut être inversé pour estimer la conductivité électrique, la perméabilité magnétique d'une cible homogène et/ou la distance capteur-cible. La procédure d'inversion a été décrite et montre une possibilité d'estimer ces trois paramètres avec précision. L'expérience a été effectuée sur des métaux magnétiques et amagnétiques.

  18. Condensation and localization of the partitioning protein ParB on the bacterial chromosome.

    Science.gov (United States)

    Broedersz, Chase P; Wang, Xindan; Meir, Yigal; Loparo, Joseph J; Rudner, David Z; Wingreen, Ned S

    2014-06-17

    The ParABS system mediates chromosome segregation and plasmid partitioning in many bacteria. As part of the partitioning mechanism, ParB proteins form a nucleoprotein complex at parS sites. The biophysical basis underlying ParB-DNA complex formation and localization remains elusive. Specifically, it is unclear whether ParB spreads in 1D along DNA or assembles into a 3D protein-DNA complex. We show that a combination of 1D spreading bonds and a single 3D bridging bond between ParB proteins constitutes a minimal model for a condensed ParB-DNA complex. This model implies a scaling behavior for ParB-mediated silencing of parS-flanking genes, which we confirm to be satisfied by experimental data from P1 plasmids. Furthermore, this model is consistent with experiments on the effects of DNA roadblocks on ParB localization. Finally, we show experimentally that a single parS site is necessary and sufficient for ParB-DNA complex formation in vivo. Together with our model, this suggests that ParB binding to parS triggers a conformational switch in ParB that overcomes a nucleation barrier. Conceptually, the combination of spreading and bridging bonds in our model provides a surface tension ensuring the condensation of the ParB-DNA complex, with analogies to liquid-like compartments such as nucleoli in eukaryotes.

  19. Centromere pairing by a plasmid-encoded type I ParB protein

    DEFF Research Database (Denmark)

    Ringgaard, Simon; Löwe, Jan; Gerdes, Kenn

    2007-01-01

    over the nucleoid. ParB ribbon-helix-helix dimers bind cooperatively to direct repeats in parC1 and parC2. Using four different assays we obtain solid evidence that ParB can pair parC1- and parC2-encoding DNA fragments in vitro. Convincingly, electron microscopy revealed that ParB mediates binary...... pairing of parC fragments. In addition to binary complexes, ParB mediated the formation of higher order complexes consisting of several DNA fragments joined by ParB at centromere site parC. N-terminal truncated versions of ParB still possessing specific DNA binding activity were incompetent in pairing...

  20. Soluble urokinase plasminogen activator receptor (suPAR) is a novel, independent predictive marker of myocardial infarction in HIV-1-infected patients

    DEFF Research Database (Denmark)

    Rasmussen, Line Jee Hartmann; Knudsen, A; Katzenstein, T L

    2016-01-01

    , gender, duration of antiretroviral therapy (ART), smoking and no known CVD. suPAR was measured in the four plasma samples available for each patient at different time-points; 1, Before initiation of ART; 2, 3 months after initiation of ART; 3, 1 year before the case's MI; and 4, The last sample available......PAR could be a useful biomarker for prediction of first-time MI in this patient group, even years before the event....... in the general population. We tested suPAR as a predictive biomarker of MI in HIV-1-infected individuals. METHODS: suPAR levels were investigated in a nested case-control study of 55 HIV-1-infected cases with verified first-time MI and 182 HIV-1-infected controls with no known CVD. Controls were matched for age...

  1. Role of suPAR and Lactic Acid in Diagnosing Sepsis and Predicting Mortality in Elderly Patients

    Science.gov (United States)

    Khater, Walaa S.; Salah-Eldeen, Noha N.; Khater, Mohamed S.; Saleh, Asghraf N.

    2016-01-01

    This study investigated the diagnostic value of soluble urokinase plasminogen activator receptor (suPAR) and serum lactate in elderly patients with sepsis and evaluated their capacity to predict mortality and their correlation to Sequential Organ Failure Assessment (SOFA) score. The study included 80 participants, divided into two groups: 40 cases (mean age, 68.9 ± 5.9) admitted to the intensive care unit and 40 healthy controls (mean age, 67.1 ± 6.2). Elderly patients with sepsis had significantly higher levels of serum suPAR and lactic acid compared to healthy controls. Receiver operating characteristic (ROC) curve analysis showed that suPAR (cutoff value, ≥4.37 ng/ml) has higher area under the curve (AUC) than lactic acid (cutoff value, ≥1.95 mmol/l) for diagnosing sepsis. Serum lactate has superior prognostic value compared to suPAR with AUC of 0.82 (cutoff value, 2.2 mmol/l) and 0.72 (cutoff value, 6.3 ng/ml), respectively. The diagnostic power of combined usage of suPAR and lactate serum concentrations showed AUC of 0.988 (95% confidence interval 0.934 to 1.0). The combination of both biomarkers either together or with SOFA score may serve as a useful guide to patients who need more intensive resuscitation.

  2. uPAR as anti-cancer target

    DEFF Research Database (Denmark)

    Lund, Ida K; Illemann, Martin; Thurison, Tine

    2011-01-01

    , and a potential diagnostic and predictive impact of the different uPAR forms has been reported. Hence, pericellular proteolysis seems to be a suitable target for anti-cancer therapy and numerous approaches have been pursued. Targeting of this process may be achieved by preventing the binding of uPA to u...... using mouse monoclonal antibodies (mAbs) against mouse uPA or uPAR. These reagents will target uPA and uPAR in both stromal cells and cancer cells, and their therapeutic potential can now be assessed in syngenic mouse cancer models....

  3. Limnological database for Par Pond: 1959 to 1980

    Energy Technology Data Exchange (ETDEWEB)

    Tilly, L.J.

    1981-03-01

    A limnological database for Par Pond, a cooling reservoir for hot reactor effluent water at the Savannah River Plant, is described. The data are derived from a combination of research and monitoring efforts on Par Pond since 1959. The approximately 24,000-byte database provides water quality, primary productivity, and flow data from a number of different stations, depths, and times during the 22-year history of the Par Pond impoundment. The data have been organized to permit an interpretation of the effects of twenty years of cooling system operations on the structure and function of an aquatic ecosystem.

  4. The inflammatory marker suPAR after cardiac arrest

    DEFF Research Database (Denmark)

    Rundgren, Malin; Lyngbaek, Stig; Fisker, Helle

    2015-01-01

    BACKGROUND: Soluble urokinase plasminogen activator receptor (suPAR) is released in response to inflammatory stimuli, and plasma levels are associated with long-term outcomes. The ischemia/reperfusion injury caused by cardiac arrest (CA) and resuscitation triggers an inflammatory response...... analysis shoved an AUC of 0.76 at 6 hours. In the subgroup of CA of cardiac cause, the AUC was 0.84. CONCLUSION: suPAR levels at 6 and 36 hours after CA were significantly higher in nonsurviving patients compared with survivors; however, the overlap in suPAR levels between the outcome groups...

  5. Predicted PAR1 inhibitors from multiple computational methods

    Science.gov (United States)

    Wang, Ying; Liu, Jinfeng; Zhu, Tong; Zhang, Lujia; He, Xiao; Zhang, John Z. H.

    2016-08-01

    Multiple computational approaches are employed in order to find potentially strong binders of PAR1 from the two molecular databases: the Specs database containing more than 200,000 commercially available molecules and the traditional Chinese medicine (TCM) database. By combining the use of popular docking scoring functions together with detailed molecular dynamics simulation and protein-ligand free energy calculations, a total of fourteen molecules are found to be potentially strong binders of PAR1. The atomic details in protein-ligand interactions of these molecules with PAR1 are analyzed to help understand the binding mechanism which should be very useful in design of new drugs.

  6. Sistema para medir en vehículo el alabeo, variación de espesor y par residual de los frenos de disco

    OpenAIRE

    2015-01-01

    En cualquier vehículo equipado con frenos de disco aparecen anomalías como el alabeo y la variación de espesor de los discos y el par residual del sistema de freno. La medición de estos parámetros requiere de un sistema automatizado que permita medidas fiables independientes del operador. Este proyecto muestra el diseño de un sistema de control, adquisición y procesado de datos automático, que facilita la captura de dichos parámetros para su posterior análisis. El proyecto ha sido re...

  7. Purification by high vacuum fusion and progressive solidification of uranium from electrolytic origin; Purification par fusion sous vide eleve et solidification progressive d'uranium d'origine electrolytique

    Energy Technology Data Exchange (ETDEWEB)

    Poeydomenge, P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-01-15

    ;dissociative growth' of the grain observed, so-called secondary recrystallisation. in appendix, the method for measuring the electrical resistance by induction (with direct current)is studied from the fundamental and experimental point of view. The author applied it to the particular case of uranium for measuring the low-temperature resistance of the bars from the same which solidified first to the least pure ended the ingot. (author) [French] Dans le cadre de recherches generales sur la purification de l'uranium par fusion de zone, on a entrepris de determiner le degre de purification que l'on pourrait atteindre par une simple solidification progressive a vitesse et direction soigneusement controlees d'un uranium de purete nucleaire courante. Cet uranium de purete intermediaire fournirait un materiau de depart approprie au mode de purification ultime qu'est la fusion a zone verticale, dite ''flottante''. Dans ce but, des lingots d'uranium d'origine electrolytique ont ete refondus sous vide (2 a 5 x 10{sup -6} mm) dans une longue nacelle en UO{sub 2} apres une monte lente en temperature pour eliminer le maximum de gaz et d'impuretes volatiles. Ce degazage et cette volatilisation d'impuretes sont completes par maintien prolonge a haute temperature du bais liquide. Celui-ci est ensuite solidifie d'une extremite a l'autre de la nacelle par deplacement a vitesse lente et constante du front de solidification de facon a obtenir une repartition des impuretes selon les lois etablies par PFANN. Differentes methodes experimentales ont permis de montrer que le metal solidifie en premier lieu est nettement plus pur que celui de la partie solidifie a l'extremite opposee du lingot. Le degre de purification du metal en tete du lingot a ete apprecie, soit quantitativement par mesure du rapport des resistivites electriques a la temperature ambiante et a celle de l'azote liquide, soit qualitativement par l

  8. A turquesa de Itacupim, Pará

    Directory of Open Access Journals (Sweden)

    Marcondes Lima da Costa

    2004-12-01

    Full Text Available Na ilha de Itacupim, localizada na região costeira do nordeste do Pará, foram encontrados veios de fosfatos de alumínio contendo turquesa, além de quartzo e argilominerais. A ilha é sustentada por espesso perfil laterítico maturo desenvolvido sobre complexo alcalino-ultramáfico mineralizado em apatita. Os veios e vênulas são de espessura centimétrica, normalmente constituídos de wavellita fibro-radial, onde pode ser observada turquesa verde-azulada, em massas subesferolíticas, microcristalinas, intercrescidas com caulinita e oxi-hidróxidos de Mn, além de quartzo. A identificação mineral foi realizada por DRX, microscopia óptica, análises químicas de rocha total, MEV/SED. Os teores de CuO são inferiores aos das turquesas em geral, compensados por Fe2O3 e ZnO. Os subesferolitos de turquesa contêm inúmeras inclusões micrométricas de goyazita ou svanbergita. A ocorrência da turquesa, na forma de veios e vênulas, seu aspecto porcelanado e a conhecida relação desse mineral com ambiente hidrotermal sugerem que a turquesa de Itacupim também seja de origem hidrotermal, reforçada pela sua associação com wavellita, goyazita ou svanbergita, quartzo e argilominerais. Ela não foi encontrada no perfil laterítico. Seu aspecto compacto e sua cor esverdeada abrem perspectivas para seu uso como mineral de gema.Veins and veinlets of aluminum phosphates with turquoise occur at the Itacupim Island in the coastal plain northeast the state of Pará. A thick mature lateritic iron crust rich in aluminum phosphates developed an apatite-bearing alkaline-ultramafic complex which constitutes the Island. The veins and veinlets are cm-thick, usually constituted by wavellite, fibrous to radialfibrous, with bony or porcelaneous aspect, and can turquoise. Pebbles of these phosphates inside of apatite-bearing ultramafic rocks are very common at the base of the hang wall, and locally form expressive agglomerates. Turquoise forms half spheroids and

  9. Parálisis cerebral Cerebral palsy

    Directory of Open Access Journals (Sweden)

    Jorge Malagon Valdez

    2007-01-01

    Full Text Available El término parálisis cerebral (PC engloba a un gran número de síndromes neurológicos clínicos, de etiología diversa. Estos síndromes se caracterizan por tener una sintomatología común: los trastornos motores. Algunos autores prefieren manejar términos como "encefalopatía fija", "encefalopatías no evolutivas". Se mencionan la utilidad de programas de intervención temprana y métodos especiales de rehabilitación, así como el manejo de las deficiencias asociadas como la epilepsia, deficiencia mental, trastornos del lenguaje, audición, visión, déficit de la atención que mejoran el pronóstico de manera significativa. El pronóstico también depende de la gravedad del padecimiento y de las manifestaciones asociadas.The term cerebral palsy (CP, is used for a great number of clinical neurological syndromes. The syndromes are characterized by having a common cause, motor defects. It is important, because they can cause a brain damage by presenting motor defects and some associated deficiencies, such as mental deficiency, epilepsy, language and visual defects and pseudobulbar paralysis, with the nonevolving fact. Some authors prefer using terms such as "non-evolving encephalopathies". In the treatment the utility of prevention programs of early stimulation and special rehabilitation methods, and treatment of associated deficiencies such as epilepsy, mental deficiency, language, audition and visual problems, and the attention deficit improve the prognosis in an important way. The prognosis depends on the severity of the disease and the associated manifestations.

  10. Das uPAR-System: Identifizierung neuer uPAR-Interaktionspartner und ihre Relevanz beim triple-negativen Brustkrebs

    OpenAIRE

    Huber, Michaela

    2016-01-01

    In dieser Arbeit führte die dauerhafte Reduzierung der Proteinexpression von uPAR kombiniert mit uPA bzw. IGF-1R zu einem reduzierten Tumorverhalten in vitro und könnte einen Ansatz für eine bessere TNBC-Therapie darstellen. IGF-1R wurde als direkter Interaktionspartner von uPAR bestätigt. Darüber hinaus wurden Cyr61, YB-1 und Caprin-1 als neue Interaktionspartner von uPAR und als potentielle Therapieziele identifiziert. Die Expression von Cyr61 und YB-1 korrelierte signifikant mit der Expre...

  11. Attractor states, control parameters, and co-adaptation in instructed L2 inferential comprehension: a design-based research study of a critical reading intervention Estados atratores, parâmetros de controle, e co-adaptação no ensino da compreensão inferencial em L2: uma pesquisa design-based de intervenção de leitura crítica

    Directory of Open Access Journals (Sweden)

    Moisés Damián Perales Escudero

    2013-01-01

    identificação de estados atratores e de parâmetros de controle no discurso da sala de aula foram utilizados para explorar a influência de fatores imprevisíveis na compreensão dos participantes e refinar ainda mais a intervenção. Os resultados indicam que o conhecimento do gênero retórico atuou como parâmetro de controle conduzindo a compreensão dos alunos para os estados atratores caracterizados por inferências implausíveis, e que este conhecimento explica a emergência do significado pragmático (inferências retóricas a partir do significado semântico. O artigo ilustra a utilidade de construtos da teoria da complexidade para realizar pesquisa qualitativa de modo a fornecer subsídios teóricos e práticos.

  12. The use of statistical techniques in par-level management.

    Science.gov (United States)

    Klee, W B

    1994-02-01

    The total quality management movement has allowed the reintroduction of statistics in the materials management workplace. Statistical methods can be applied to the par level management process with significant results.

  13. First-in-human uPAR PET

    DEFF Research Database (Denmark)

    Persson, Morten; Skovgaard, Dorthe; Brandt-Larsen, Malene

    2015-01-01

    A first-in-human clinical trial with Positron Emission Tomography (PET) imaging of the urokinase-type plasminogen activator receptor (uPAR) in patients with breast, prostate and bladder cancer, is described. uPAR is expressed in many types of human cancers and the expression is predictive...... of invasion, metastasis and indicates poor prognosis. uPAR PET imaging therefore holds promise to be a new and innovative method for improved cancer diagnosis, staging and individual risk stratification. The uPAR specific peptide AE105 was conjugated to the macrocyclic chelator DOTA and labeled with (64)Cu...... for targeted molecular imaging with PET. The safety, pharmacokinetic, biodistribution profile and radiation dosimetry after a single intravenous dose of (64)Cu-DOTA-AE105 were assessed by serial PET and computed tomography (CT) in 4 prostate, 3 breast and 3 bladder cancer patients. Safety assessment...

  14. PAR1- and PAR2-induced innate immune markers are negatively regulated by PI3K/Akt signaling pathway in oral keratinocytes

    Directory of Open Access Journals (Sweden)

    Dale Beverly A

    2010-10-01

    Full Text Available Abstract Background Protease-Activated Receptors (PARs, members of G-protein-coupled receptors, are activated by proteolytic activity of various proteases. Activation of PAR1 and PAR2 triggers innate immune responses in human oral keratinocytes (HOKs, but the signaling pathways downstream of PAR activation in HOKs have not been clearly defined. In this study, we aimed to determine if PAR1- and PAR2-mediated signaling differs in the induction of innate immune markers CXCL3, CXCL5 and CCL20 via ERK, p38 and PI3K/Akt. Results Our data show the induction of innate immunity by PAR1 requires both p38 and ERK MAP kinases, while PAR2 prominently signals via p38. However, inhibition of PI3K enhances expression of innate immune markers predominantly via suppressing p38 phosphorylation signaled by PAR activation. Conclusion Our data indicate that proteases mediating PAR1 and PAR2 activation differentially signal via MAP kinase cascades. In addition, the production of chemokines induced by PAR1 and PAR2 is suppressed by PI3K/Akt, thus keeping the innate immune responses of HOK in balance. The results of our study provide a novel insight into signaling pathways involved in PAR activation.

  15. Melanoma cell therapy: Endothelial progenitor cells as shuttle of the MMP12 uPAR-degrading enzyme

    Science.gov (United States)

    Laurenzana, Anna; Biagioni, Alessio; D'Alessio, Silvia; Bianchini, Francesca; Chillà, Anastasia; Margheri, Francesca; Luciani, Cristina; Mazzanti, Benedetta; Pimpinelli, Nicola; Torre, Eugenio; Danese, Silvio; Calorini, Lido; Rosso, Mario Del; Fibbi, Gabriella

    2014-01-01

    The receptor for the urokinase-type plasminogen activator (uPAR) accounts for many features of cancer progression, and is therefore considered a target for anti-tumoral therapy. Only full length uPAR mediates tumor progression. Matrix-metallo-proteinase-12 (MMP12)-dependent uPAR cleavage results into the loss of invasion properties and angiogenesis. MMP12 can be employed in the field of “targeted therapies” as a biological drug to be delivered directly in patient's tumor mass. Endothelial Progenitor Cells (EPCs) are selectively recruited within the tumor and could be used as cellular vehicles for delivering anti-cancer molecules. The aim of our study is to inhibit cancer progression by engeneering ECFCs, a subset of EPC, with a lentivirus encoding the anti-tumor uPAR-degrading enzyme MMP12. Ex vivo manipulated ECFCs lost the capacity to perform capillary morphogenesis and acquired the anti-tumor and anti-angiogenetic activity. In vivo MMP12-engineered ECFCs cleaved uPAR within the tumor mass and strongly inhibited tumor growth, tumor angiogenesis and development of lung metastasis. The possibility to exploit tumor homing and activity of autologous MMP12-engineered ECFCs represents a novel way to combat melanoma by a “personalized therapy”, without rejection risk. The i.v. injection of radiolabelled MMP12-ECFCs can thus provide a new theranostic approach to control melanoma progression and metastasis. PMID:25003596

  16. Melanoma cell therapy: Endothelial progenitor cells as shuttle of the MMP12 uPAR-degrading enzyme.

    Science.gov (United States)

    Laurenzana, Anna; Biagioni, Alessio; D'Alessio, Silvia; Bianchini, Francesca; Chillà, Anastasia; Margheri, Francesca; Luciani, Cristina; Mazzanti, Benedetta; Pimpinelli, Nicola; Torre, Eugenio; Danese, Silvio; Calorini, Lido; Del Rosso, Mario; Fibbi, Gabriella

    2014-06-15

    The receptor for the urokinase-type plasminogen activator (uPAR) accounts for many features of cancer progression, and is therefore considered a target for anti-tumoral therapy. Only full length uPAR mediates tumor progression. Matrix-metallo-proteinase-12 (MMP12)-dependent uPAR cleavage results into the loss of invasion properties and angiogenesis. MMP12 can be employed in the field of "targeted therapies" as a biological drug to be delivered directly in patient's tumor mass. Endothelial Progenitor Cells (EPCs) are selectively recruited within the tumor and could be used as cellular vehicles for delivering anti-cancer molecules. The aim of our study is to inhibit cancer progression by engeneering ECFCs, a subset of EPC, with a lentivirus encoding the anti-tumor uPAR-degrading enzyme MMP12. Ex vivo manipulated ECFCs lost the capacity to perform capillary morphogenesis and acquired the anti-tumor and anti-angiogenetic activity. In vivo MMP12-engineered ECFCs cleaved uPAR within the tumor mass and strongly inhibited tumor growth, tumor angiogenesis and development of lung metastasis. The possibility to exploit tumor homing and activity of autologous MMP12-engineered ECFCs represents a novel way to combat melanoma by a "personalized therapy", without rejection risk. The i.v. injection of radiolabelled MMP12-ECFCs can thus provide a new theranostic approach to control melanoma progression and metastasis.

  17. Par-4 secretion: stoichiometry of 3-arylquinoline binding to vimentin.

    Science.gov (United States)

    Sviripa, Vitaliy M; Burikhanov, Ravshan; Obiero, Josiah M; Yuan, Yaxia; Nickell, Justin R; Dwoskin, Linda P; Zhan, Chang-Guo; Liu, Chunming; Tsodikov, Oleg V; Rangnekar, Vivek M; Watt, David S

    2016-01-01

    Advanced prostate tumors usually metastasize to the lung, bone, and other vital tissues and are resistant to conventional therapy. Prostate apoptosis response-4 protein (Par-4) is a tumor suppressor that causes apoptosis in therapy-resistant prostate cancer cells by binding specifically to a receptor, Glucose-regulated protein-78 (GRP78), found only on the surface of cancer cells. 3-Arylquinolines or "arylquins" induce normal cells to release Par-4 from the intermediate filament protein, vimentin and promote Par-4 secretion that targets cancer cells in a paracrine manner. A structure-activity study identified arylquins that promote Par-4 secretion, and an evaluation of arylquin binding to the hERG potassium ion channel using a [(3)H]-dofetilide binding assay permitted the identification of structural features that separated this undesired activity from the desired Par-4 secretory activity. A binding study that relied on the natural fluorescence of arylquins and that used the purified rod domain of vimentin (residues 99-411) suggested that the mechanism behind Par-4 release involved arylquin binding to multiple sites in the rod domain.

  18. Disrupting the right pars opercularis with electrical stimulation frees the song: case report.

    Science.gov (United States)

    Herbet, Guillaume; Lafargue, Gilles; Almairac, Fabien; Moritz-Gasser, Sylvie; Bonnetblanc, François; Duffau, Hugues

    2015-12-01

    The authors report the first case of a strikingly unusual speech impairment evoked by intraoperative electrostimulation in a 36-year-old right-handed patient, a well-trained singer, who underwent awake surgery for a right fronto-temporo-insular low-grade glioma. Functionally disrupting the pars opercularis of the right inferior frontal gyrus led the patient to automatically switch from a speaking to a singing mode of language production. Given the central role of the right pars opercularis in the inhibitory control network, the authors propose that this finding may be interpreted as possible evidence for a competitive and independent neurocognitive subnetwork devoted to the melodically intoned articulation of words (normal language-based vs singing-based) in subjects with high expertise. From a more clinical perspective, such data may have implications for awake neurosurgery, especially to preserve the quality of life for singers.

  19. Solar water heating at Ikea's Montreal store; Chauffage solaire de l'eau courante au magasin Ikea de Montreal

    Energy Technology Data Exchange (ETDEWEB)

    Lagana, A.; Sonmor, K. [Ecovision experts-conseils inc., Montreal, PQ (Canada)

    2010-01-01

    The Ikea store in Montreal has integrated an energy efficient solar water heater into its building infrastructure. In June 2010, 30 fixed solar panels were installed on the roof to recuperate energy from the sun for hot water use in the restaurant and store facilities. Prior to this installation, the store was using natural gas for water heating purposes. During a sunny summer day, the solar water heater can provide 100 percent of the hot water needs of the store, even though it was designed to provide only one-third of the heating requirements. The panels are made of copper and painted with black titanium paint. A solution of water and antifreeze circulates in the panels. Digital controls are used at the pumps to control the temperature. The Ikea store uses approximately 3,100 gallons of water per day. The panels provide more than 60 MWh of energy, and represent a savings of 8440 cubic metres of natural gas, and a savings of 19.7 tons of carbon dioxide. The panels cost $1,300 and the return on investment is estimated to be 15.6 years. 3 figs.

  20. Pars plana vitrectomy and silicone oil tamponade for acute endophthalmitis treatment Vitrectomia pars plana e tamponamento por óleo de silicone para o tratamento de endoftalmite aguda

    Directory of Open Access Journals (Sweden)

    Rubens Camargo Siqueira

    2009-02-01

    Full Text Available PURPOSE: To evaluate the outcomes of pars plana vitrectomy and silicone oil injection for the treatment of infectious endophthalmitis. METHODS: 35 cases of endophthalmitis secondary to phacoemulsification (20 patients, trabeculectomy (8 patients, perforating trauma (2 patients, trauma (2 patients, corneal transplantation (1 patient, vitrectomy (1 patient and corneal ulceration (1 patient were retrospectively studied. Patients were separated into two groups: Group 1 (n=24: intravitreal antibiotic injection, associated with topical and oral antibiotics; Group 2 (n=11: vitrectomy with intravitreal antibiotic injection and silicone oil injection. The follow-up ranged from 1 to 48 months (mean of 16 months. RESULTS: From 24 patients in group 1, 11 patients (45.83%, had infection controlled with intravitreal antibiotic injection only; 13 patients (54.15% regressed to uncontrolled endophthalmitis, in which two patients (8.33% were submitted to evisceration and one patient (4.16% had corneal melting. The remaining 10 patients (41.66% with uncontrolled endophthalmitis were submitted to pars plana vitrectomy and silicone oil injection. Six patients (25% from Group I had retinal detachment during the first month of follow-up and also required pars plana vitrectomy and silicone oil injection. In Group 2 patients (n=11, all of them had controlled infection at the first procedure. In one case (9.09%, a severe proliferatative vitreoretinopathy induced loss of vision. CONCLUSION: These results suggest that silicone oil tamponade might be beneficial in the treatment strategy of infectious endophthalmitis.OBJETIVO: Avaliar os resultados da vitrectomia pars plana com tamponamento com óleo de silicone no tratamento de endoftalmite aguda. MÉTODOS: Trinta e cinco pacientes com endoftalmite, sendo 20 secundário à facoemulsificação, 8 por trabeculectomia, 2 por trauma perfurante, 2 por trauma, 1 por transplante de córnea, 1 por vitrectomia, e 1 por úlcera de c

  1. Une terreur par l’image

    Directory of Open Access Journals (Sweden)

    Annie DULONG

    2011-09-01

    Full Text Available Si d’autres événements — Hiroshima, la découverte des camps de concentration, la chute du mur de Berlin — ont, au cours du XXe siècle, marqué suffisamment l’imaginaire pour trouver leur réponse dans des œuvres d’art, le 11 septembre 2001 pose la question de la fictionnalisation autrement : il ne s’agit plus tant de combler les manques de la représentation, liés à l’absence d’images ou au délai dans leur dévoilement, mais bien de composer avec l’omniprésence d’une représentation martelée à la télévision et sur Internet. Que cette représentation soit partielle, tronquée et qu’il lui manque la présence des corps, ces grands absents des photographies et des images tournées par les journalistes, ne change rien au fait que c’est avec le trop-plein que les artistes doivent maintenant négocier.Cet article s’intéresse aux romans qui traitent l’événement de front au lieu de l’aborder sur un mode mineur, et a pour objectif de tracer un portrait de la représentation des médias dans les romans du 11 septembre 2001. Ces romans, outre le fait qu’ils traitent des événements de New York, ont comme point commun un personnage, ou décor : les médias, représentés par la télévision, l’Internet, les photographies de presse, interviennent dans les récits d’une manière significative et témoignent de la force brute des images. À travers une étude de certains des mécanismes à l’œuvre dans The Writing on the Wall (Lynne Sharon Schwartz, Extremely Loud and Incredibly Close (Jonathan Safran Foer, Falling Man (Don DeLillo et A Disorder Peculiar to the Country (Ken Kalfus, il s’agira de proposer des réponses à ces quelques questions : quel rôle les médias jouent-ils, et comment participent-ils au récit ? Quelle pression les images exercent-elles sur les personnages ? Que révèlent les personnages enfants dans leur rapport aux médias ? Quelle critique, finalement, les romans

  2. Intrinsic and integrative properties of substantia nigra pars reticulata neurons

    Science.gov (United States)

    Zhou, Fu-Ming; Lee, Christian R.

    2011-01-01

    The GABA projection neurons of the substantia nigra pars reticulata (SNr) are output neurons for the basal ganglia and thus critical for movement control. Their most striking neurophysiological feature is sustained, spontaneous high frequency spike firing. A fundamental question is: what are the key ion channels supporting the remarkable firing capability in these neurons? Recent studies indicate that these neurons express tonically active TRPC3 channels that conduct a Na-dependent inward current even at hyperpolarized membrane potentials. When the membrane potential reaches −60 mV, a voltage-gated persistent sodium current (INaP) starts to activate, further depolarizing the membrane potential. At or slightly below −50 mV, the large transient voltage-activated sodium current (INaT) starts to activate and eventually triggers the rapid rising phase of action potentials. SNr GABA neurons have a higher density of (INaT), contributing to the faster rise and larger amplitude of action potentials, compared with the slow-spiking dopamine neurons. INaT also recovers from inactivation more quickly in SNr GABA neurons than in nigral dopamine neurons. In SNr GABA neurons, the rising phase of the action potential triggers the activation of high-threshold, inactivation-resistant Kv3-like channels that can rapidly repolarize the membrane. These intrinsic ion channels provide SNr GABA neurons with the ability to fire spontaneous and sustained high frequency spikes. Additionally, robust GABA inputs from direct pathway medium spiny neurons in the striatum and GABA neurons in the globus pallidus may inhibit and silence SNr GABA neurons, whereas glutamate synaptic input from the subthalamic nucleus may induce burst firing in SNr GABA neurons. Thus, afferent GABA and glutamate synaptic inputs sculpt the tonic high frequency firing of SNr GABA neurons and the consequent inhibition of their targets into an integrated motor control signal that is further fine-tuned by neuromodulators

  3. Rapid evaluation of the neutron dose following a criticality accident by measurement of {sup 24}Na activity; Evaluation rapide de la dose de neutrons a la suite d'un accident de criticite par mesure de l'activite de {sup 24}Na

    Energy Technology Data Exchange (ETDEWEB)

    Estournel, R. [Centre de Production de Plutonium de Marcoule, Service de Protection contre les Rayonnements, 30 (France); Henry, Ph. [Centre de Production de Plutonium de Marcoule, Section Medicale et Sociale, 30 (France); Beau, P.; Ergas, A. [Commissariat a l' Energie Atomique, Service d' Hygiene Atomique, Dept. de la Protection Sanitaire, Chusclan, (France)

    1966-07-01

    By external measurement of the gamma activity of {sup 24}Na induced in the human organs by a neutron flux during a criticality accident, it is possible to evaluate the personal dose received. Detectors designed for everyday use in health physics can be applied to these measurements, and this is described in the first part of the work. The response of a certain number of induced-activity detectors is presented. The induced activity-dose relationship is studied theoretically in the second part taking into account the neutron spectrum to which the individual has been subjected. The characteristic spectra of three possible types of accident have been used for deducing this relationship. The results obtained show that the method is sufficiently sensitive for present purposes. The accuracy of this method for calculating the dose received during an experiment is discussed. (authors) [French] La mesure par detection externe de l'activite gamma du sodium 24 induit dans l'organisme humain par un flux de neutrons lors d'un accident de criticite rend possible l'evaluation de la dose recue par un individu irradie. L'utilisation de detecteurs d'un emploi courant en radioprotection fait l'objet d'une experimentation qui constitue la premiere partie de cette etude. La reponse d'un certain nombre de detecteurs a une activite induite connue est presentee. La relation dose-activite induite, est etudiee, de maniere theorique, dans la seconde partie, correlativement au spectre des neutrons qui ont atteint l'individu irradie. Les spectres caracteristiques de trois types d'accidents possibles ont ete retenus pour l'etablissement de ces relations. Les resultats obtenus montrent que la methode satisfait avec une sensibilite suffisante au but recherche. La precision avec laquelle on peut ainsi calculer la dose recue au cours d'un accident de criticite est discutee. (auteurs)

  4. ViPAR: a software platform for the Virtual Pooling and Analysis of Research Data.

    Science.gov (United States)

    Carter, Kim W; Francis, Richard W; Carter, K W; Francis, R W; Bresnahan, M; Gissler, M; Grønborg, T K; Gross, R; Gunnes, N; Hammond, G; Hornig, M; Hultman, C M; Huttunen, J; Langridge, A; Leonard, H; Newman, S; Parner, E T; Petersson, G; Reichenberg, A; Sandin, S; Schendel, D E; Schalkwyk, L; Sourander, A; Steadman, C; Stoltenberg, C; Suominen, A; Surén, P; Susser, E; Sylvester Vethanayagam, A; Yusof, Z

    2015-10-08

    Research studies exploring the determinants of disease require sufficient statistical power to detect meaningful effects. Sample size is often increased through centralized pooling of disparately located datasets, though ethical, privacy and data ownership issues can often hamper this process. Methods that facilitate the sharing of research data that are sympathetic with these issues and which allow flexible and detailed statistical analyses are therefore in critical need. We have created a software platform for the Virtual Pooling and Analysis of Research data (ViPAR), which employs free and open source methods to provide researchers with a web-based platform to analyse datasets housed in disparate locations. Database federation permits controlled access to remotely located datasets from a central location. The Secure Shell protocol allows data to be securely exchanged between devices over an insecure network. ViPAR combines these free technologies into a solution that facilitates 'virtual pooling' where data can be temporarily pooled into computer memory and made available for analysis without the need for permanent central storage. Within the ViPAR infrastructure, remote sites manage their own harmonized research dataset in a database hosted at their site, while a central server hosts the data federation component and a secure analysis portal. When an analysis is initiated, requested data are retrieved from each remote site and virtually pooled at the central site. The data are then analysed by statistical software and, on completion, results of the analysis are returned to the user and the virtually pooled data are removed from memory. ViPAR is a secure, flexible and powerful analysis platform built on open source technology that is currently in use by large international consortia, and is made publicly available at [http://bioinformatics.childhealthresearch.org.au/software/vipar/]. © The Author 2015. Published by Oxford University Press on behalf of the

  5. Synthesis of the IRSN analysis of the control-command architecture and platforms of the Flamanville 3 EPR reactor; Synthese de l'analyse par l'IRSN de l'architecture et des plateformes du controle-commande du reacteur EPR de Flamanville 3

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-06-16

    This document first briefly describes the context: control-command systems and functions, examples of control-command systems of the EPR, control-command platforms, and control-command architecture. It describes the method implemented by the IRSN to analyze the reactor control-command: assessment of the capacity of each system to perform its functions, assessment of the control-command architecture, hardware qualification. Then, it briefly recalls the assessments performed for the Teleperm XS platform and for the SPPA-T2000 platform, for the architecture (separation of systems belonging to different classes, in-depth defence, management of the architecture complexity)

  6. Developpement de techniques de diagnostic non intrusif par tomographie optique

    Science.gov (United States)

    Dubot, Fabien

    Que ce soit dans les domaines des procedes industriels ou de l'imagerie medicale, on a assiste ces deux dernieres decennies a un developpement croissant des techniques optiques de diagnostic. L'engouement pour ces methodes repose principalement sur le fait qu'elles sont totalement non invasives, qu'elle utilisent des sources de rayonnement non nocives pour l'homme et l'environnement et qu'elles sont relativement peu couteuses et faciles a mettre en oeuvre comparees aux autres techniques d'imagerie. Une de ces techniques est la Tomographie Optique Diffuse (TOD). Cette methode d'imagerie tridimensionnelle consiste a caracteriser les proprietes radiatives d'un Milieu Semi-Transparent (MST) a partir de mesures optiques dans le proche infrarouge obtenues a l'aide d'un ensemble de sources et detecteurs situes sur la frontiere du domaine sonde. Elle repose notamment sur un modele direct de propagation de la lumiere dans le MST, fournissant les predictions, et un algorithme de minimisation d'une fonction de cout integrant les predictions et les mesures, permettant la reconstruction des parametres d'interet. Dans ce travail, le modele direct est l'approximation diffuse de l'equation de transfert radiatif dans le regime frequentiel tandis que les parametres d'interet sont les distributions spatiales des coefficients d'absorption et de diffusion reduit. Cette these est consacree au developpement d'une methode inverse robuste pour la resolution du probleme de TOD dans le domaine frequentiel. Pour repondre a cet objectif, ce travail est structure en trois parties qui constituent les principaux axes de la these. Premierement, une comparaison des algorithmes de Gauss-Newton amorti et de Broyden- Fletcher-Goldfarb-Shanno (BFGS) est proposee dans le cas bidimensionnel. Deux methodes de regularisation sont combinees pour chacun des deux algorithmes, a savoir la reduction de la dimension de l'espace de controle basee sur le maillage et la regularisation par penalisation de Tikhonov

  7. Pars Plana Vitrectomy in Treatment of Lens Injury

    Directory of Open Access Journals (Sweden)

    Remzi Avcı

    2013-01-01

    Full Text Available Lens injury due to traumas may present as loss of capsule integrity, cataract, lens subluxation, or lens luxation. In addition, lens subluxation and lens luxation may occur in pseudophakic patients due to trauma. Clear corneal, scleral or sclero-corneal phacoemulsification surgery, pars plana vitrectomy, pars plana vitrectomy with phaco-fragmentation, or pars plana vitrectomy with removal through corneal incision techniques are used in the treatment of lens injury due to traumas. Intraocular lens can be implanted in the bag, sulcus, or anterior chamber during the surgery. Depending on the circumstances, scleral fixated intraocular lenses or iris-claw lenses may also be preferred. Rehabilitation of such patients is not of a great concern today, with the advanced level of vitreoretinal surgery and cataract surgery techniques and with the current state of technology. (Turk J Ophthalmol 2013; 43: 51-4

  8. The bacterial chromosome segregation protein Spo0J spreads along DNA from parS nucleation sites.

    Science.gov (United States)

    Murray, Heath; Ferreira, Henrique; Errington, Jeff

    2006-09-01

    Regulation of chromosome inheritance is essential to ensure proper transmission of genetic information. To accomplish accurate genome segregation, cells organize their chromosomes and actively separate them prior to cytokinesis. In Bacillus subtilis the Spo0J protein is required for accurate chromosome segregation and it regulates the developmental switch from vegetative growth to sporulation. Spo0J is a DNA-binding protein that recognizes at least eight identified parS sites located near the origin of replication. As judged by fluorescence microscopy, Spo0J forms discrete foci associated with the oriC region of the chromosome throughout the cell cycle. In an attempt to determine the mechanisms utilized by Spo0J to facilitate productive chromosome segregation, we have investigated the DNA binding activity of Spo0J. In vivo we find Spo0J associates with several kilobases of DNA flanking its specific binding sites (parS) through a parS-dependent nucleation event that promotes lateral spreading of Spo0J along the chromosome. Using purified components we find that Spo0J has the ability to coat non-specific DNA substrates. These 'Spo0J domains' provide large structures near oriC that could potentially demark, organize or localize the origin region of the chromosome.

  9. Cyclanthaceae no estado do Pará, Brasil Cyclanthaceae in Pará State, Brazil

    Directory of Open Access Journals (Sweden)

    Eduardo da Silva Leal

    2012-12-01

    Full Text Available Cyclanthaceae apresenta distribuição exclusiva na região neotropical sendo bem diversificada nas terras baixas das florestas pluviais tropicais, ocorrendo do sul do México até a Mata Atlântica do sul do Brasil. Consistem de ervas terrestres, epífitas, hemiepífitas ou lianas. O tratamento taxonômico de Cyclanthaceae no Pará foi baseado em coleções de herbário, trabalhos de campo e consulta a bibliografia referente à família. Foram registradas 11 espécies: Asplundia altiscandens E.S. Leal, A. fanshawei (Maguire Harling, A. glandulosa (Gleason Harling, A. heteranthera Harling, A. latifrons (Drude Harling, A. schizotepala Harling, A. xiphophylla Harling, Cyclanthus bipartitus Poit. ex A. Rich., Evodianthus funifer (Poit. Lindm., Ludovia lancifolia Brongn. e Thoracocarpus bissectus (Vell. Harling. São apresentadas chave de identificação, descrições e ilustrações das espécies, além de comentários sobre distribuição geográfica, hábitat e período de floração e frutificação.Cyclanthaceae is a family with a distribution exclusively in the Neotropics and is well diversified in lowland rainforests, occurring from southern Mexico to the Atlantic Forest of southern Brazil. The family consists of terrestrial herbs, epiphytes, hemiepiphytes and lianas. This taxonomic treatment of the Cyclanthaceae from the state of Pará was based on herbarium specimens, fieldwork, and the literature. Eleven species were recorded: Asplundia altiscandens E.S. Leal, A. fanshawei (Maguire Harling, A. glandulosa (Gleason Harling, A. heteranthera Harling, A. latifrons (Drude Harling, A. schizotepala Harling, A. xiphophylla Harling, Cyclanthus bipartitus Poit. ex A. Rich., Evodianthus funifer (Poit. Lindm., Ludovia lancifolia Brongn. and Thoracocarpus bissectus (Vell. Harling. An identification key, descriptions and illustrations of species are presented. Additional information on the geographic distribution, habitat, and flowering and

  10. Pars Plana Vitrectomy and Intravitreal Phacoemulsification for Dropped Nuclei

    Directory of Open Access Journals (Sweden)

    Khalil Ghasemi Falavarjani

    2012-01-01

    Full Text Available Purpose: To report the outcomes of pars plana vitrectomy (PPV and intravitreal phacoemulsification in patients with dropped nuclei/nuclear fragments following complicated cataract surgery. Methods: In this retrospective case series, charts of patients who had undergone PPV and intravitreal phacoemulsification for removal of dislocated nuclei/lens fragments were reviewed. After standard PPV, a conventional phacoemulsification probe with an amputated sleeve was used for grasping and emulsifying the nucleus/nuclear fragments in mid/anterior vitreous cavity. Pre- and postoperative visual acuity, and intra- and postoperative complications were recorded. Results: A total of 22 patients with mean age of 71.1΁8.2 years were studied. Mean interval between complicated cataract surgery and PPV was 26.6΁36.5 (range: 0-120 days. Patients were followed for a mean of 105.5΁57.5 days. Preoperatively, best corrected visual acuity was 2.4΁0.6 logMAR which was improved to 1.4΁0.6 logMAR at final follow-up (P<0.001. Intraoperative complications included iatrogenic peripheral retinal breaks in three eyes. Postoperative complications consisted of epiretinal membrane formation in one eye, hypotony in one eye, and medically-controlled glaucoma in 2 eyes. Conclusion: In this series, PPV and intravitreal phacoemulsification using a conventional phacoemulsification probe in patients with dropped nuclei/nuclear fragments following complicated cataract surgery resulted in visual improvement without any complications directly attributable to the probe.

  11. uPAR as anti-cancer target

    DEFF Research Database (Denmark)

    Lund, Ida K; Illemann, Martin; Thurison, Tine

    2011-01-01

    PAR on the cell surface and/or by direct inhibition of the catalytic activity of uPA. Both strategies have been pursued and inhibition of these functions has shown effect in xenogenic cancer models. Pericellular proteolysis has also been inhibited in vivo in mouse models of wound healing and hepatic fibrinolysis......, and a potential diagnostic and predictive impact of the different uPAR forms has been reported. Hence, pericellular proteolysis seems to be a suitable target for anti-cancer therapy and numerous approaches have been pursued. Targeting of this process may be achieved by preventing the binding of uPA to u...

  12. Ejercicios activos en la parálisis facial

    OpenAIRE

    Bohoyo Aramburu, Cristina

    2013-01-01

    Diseño: Se trata de una revisión bibliográfica en la que se incluyen revisiones sistemáticas y artículos sobre el tratamiento de la parálisis facial mediante ejercicios faciales, que en su conjunto engloban lo que se denomina la “mímicoterapia”. Objetivos: el objetivo principal es determinar la eficacia de los ejercicios faciales activos en el tratamiento de la parálisis de Bell. El objetivo secundario es describir dichos ejercicios. Métodos: se ha realizado una búsqueda mediante la base d...

  13. The double par locus of virulence factor pB171: DNA segregation is correlated with oscillation of ParA

    DEFF Research Database (Denmark)

    Ebersbach, G; Gerdes, K; Charbon, Gitte Ebersbach

    2001-01-01

    Prokaryotic plasmids and chromosomes encode partitioning (par) loci that segregate DNA to daughter cells before cell division. Recent database analyses showed that almost all known par loci encode an ATPase and a DNA-binding protein, and one or more cis-acting regions where the proteins act. All...... par-encoded ATPases belong to one of two protein superfamilies, Walker-type and actin-like ATPases. This property was recently used to divide par loci into Types I and II loci. We show here that the Escherichia coli virulence factor pB171 encodes a double par locus that consists of one Type I and one...

  14. Posturografia na análise do equilíbrio em crianças com paralisia cerebral: revisão de literatura Posturografía en el análisis del equilibrio en niños con parálisis cerebral: revisión de literatura Posturography in the analysis of postural control in children with cerebral palsy: a literature review

    Directory of Open Access Journals (Sweden)

    Guilherme Henrique Ramos Lopes

    2013-03-01

    Full Text Available Crianças com paralisia cerebral (PC apresentam menor equilíbrio postural pelo comprometimento de funções motoras, sensoriais e centrais. Existem diversos protocolos funcionais para análise do equilíbrio, mas poucos estudos têm utilizado a plataforma de força como instrumento de precisão nessa avaliação. O objetivo desta revisão foi identificar artigos publicados que utilizaram essa avaliação em crianças com PC e analisar os protocolos e parâmetros utilizados. Para tanto, foi realizada uma busca nas seguintes bases de dados: LILACS, IBECS, MEDLINE, Biblioteca Cochrane e SciELO, por meio da Biblioteca Virtual em Saúde. Foram encontrados 165 artigos; a partir dos critérios de inclusão e exclusão, foram selecionados 16 artigos. Os estudos mostraram que os parâmetros lineares de deslocamento médio-lateral e anteroposterior do centro de pressão e a razão desses deslocamentos têm sido utilizados com maior frequência, e que o tempo de avaliação na plataforma de força, na maioria dos estudos, não excede os 20 segundos. Foi demonstrado ainda que crianças com desenvolvimento típico apresentam melhor controle postural, e que crianças com PC podem incrementar seu equilíbrio a partir de intervenções específicas, inclusive em plataforma de força.Niños con parálisis cerebral (PC presentan menor equilibrio postural por el compromiso de sus funciones motoras, sensoriales y centrales. Existen diversos protocolos funcionales para el análisis del equilibrio, pero pocos estudios utilizan la plataforma de fuerza como instrumento de precisión en esta evaluación. El objetivo de esta revisión fue identificar artículos publicados que utilizaran esta evaluación en niños con PC y analizar los protocolos y parámetros utilizados. Por lo tanto, fue realizada una búsqueda en las siguientes bases de datos: LILACS, IBECS, MEDLINE, Biblioteca Cochrane y SciELO, por medio de la Biblioteca Virtual en Salud. Fueron encontrados 165 art

  15. Expression of the urokinase plasminogen activator receptor (uPAR) and its ligand (uPA) in brain tissues of human immunodeficiency virus patients with opportunistic cerebral diseases.

    Science.gov (United States)

    Nebuloni, Manuela; Cinque, Paola; Sidenius, Nicolai; Ferri, Angelita; Lauri, Eleonora; Omodeo-Zorini, Elisabetta; Zerbi, Pietro; Vago, Luca

    2009-01-01

    The urokinase plasminogen activator receptor (uPAR) and its ligand (uPA) play an important role in cell migration and extracellular proteolysis. We previously described uPAR/uPA overexpression in the cerebrospinal fluid (CSF) and brain tissues of patients with human immunodeficiency virus (HIV)-related cerebral diseases. In this study, we examined uPAR/uPA expression by immunohistochemistry (IHC) in brains of HIV patients with opportunistic cerebral lesions and in HIV-positive/negative controls. uPAR was found in macrophages/microglia with the highest levels in cytomegalovirus (CMV) encephalitis, toxoplasmosis, and lymphomas; in cryptococcosis and progressive multifocal leukoencephalopathy (PML) cases, only a few positive cells were found and no positivity was observed in controls. uPA expression was demonstrated only in a few macrophages/microglia and lymphocytes in all the cases and HIV-positive controls without different pattern of distribution; no uPA immunostaining was found in cryptococcosis and HIV-negative controls. The higher expression of uPAR/uPA in most of the opportunistic cerebral lesions supports their role in these diseases, suggesting their contribution to tissue injury.

  16. Le Francais Courant, Part VI: Contemporary French.

    Science.gov (United States)

    Dade County Public Schools, Miami, FL.

    This course, developed largely within the framework of units 13, 14, and 15 of the second edition of "A-LM French, Level 2," emphasizes development of basic audiolingual skills. Conversational topics permit the student to review his summer vacation. Principal elements of structure are expressions of quantity, pronouns--including those…

  17. Le Francais Courant, I (Contemporary French, I).

    Science.gov (United States)

    Dade County Public Schools, Miami, FL.

    Performance objectives focusing on the development of introductory skills in comprehension, speaking, reading, and writing are presented in this text of instructional materials. Lessons center about the use of short dialogues based on everyday, teenage experiences. Special emphasis is placed on vocabulary expansion, present tense of selected…

  18. Le Francais Courant (Contemporary French), Part II.

    Science.gov (United States)

    Dade County Public Schools, Miami, FL.

    This course has been developed basically within the limits of Units 4-6 of "A-LM French: Level 1", second edition. The primary objectives are to develop French vocabulary relative to the family, home, transportation, and foods by continuing to work with short dialogues based on everyday, teenage experiences. While reviewing previously studied…

  19. Entraînements à courant continu

    CERN Document Server

    Joos, Géza

    1987-01-01

    Caractéristiques mécaniques des moteurs et des machines entraînées. Modélisation des éléments de l'entraînement : moteur, convertisseur statique, transducteur et régulateur. Convertisseurs statiques : contraintes associées à l'alimentation des moteurs, structures, caractéristiques de fonctionnement, critères de choix, semi-conducteurs de puissance et circuits de protection. Asservissement et compréhension des boucles de régulation d'un entraînement. Nombreux exemples.

  20. The Inhibitory Effects of an Antisense u-PAR Vector on Invasion of Highly Invasive Human Prostate Carcinoma PC-3M Cell Subclones

    Institute of Scientific and Technical Information of China (English)

    廖国宁; 李清芬; 冯友梅; 邓耀祖; 李卓娅; 龚非力; 马丁

    2003-01-01

    Summary: To observe the inhibitory effects of an antisense u-PAR vector on invasion of highly inva-sive PC-3M cell subclones, the effects of the antisense u-PAR on activity of MMP-9 in those highlyinvasive cell subclones were detected by a quantitative RT-PCR and zymography. The monolayer in-vasion assay and colony formation assay in soft agar were used. And tumorigenesis rate and invasionsby the cell subclones with or without the antisense u-PAR were observed in nude mice. It was foundthat in vitro growth of highly invasive PC-3M cell subclones transfected with the antisense u-PARwas declined, and the ability of anchorage-independent growth of those cell subclones was found de-creased sharply, with the inhibiting rate becoming 79 % and 60 %, respectively. Although the anti-sense u-PAR didn't change MMP-9 gene transcription, they could inhibit the activation of MMP-9 ofhighly invasive PC-3M cell subclones. Moreover, the tumorigenesis rate of the cell subclones with theantisense u-PAR decreased and the growth of a neoplasm also slowed down. Thet tests showed thedifference between experimental and control groups was statistically significant (P<0. 01). The anti-sense u-PAR vector could not only inhibit the invasion ability of highly invasive PC-3M cell subclonesin vitro but also restrain the growth of those cell subclones in vivo.

  1. Apoplejía pituitaria con parálisis del III par craneal. Reporte de caso.

    OpenAIRE

    Pinto Valdivia, Miguel; Servicio de Endocrinología, Hospital Nacional Cayetano Heredia. Lima, Perú. Facultad de Medicina Alberto Hurtado, Universidad Peruana Cayetano Heredia. Lima.; Ortiz Torres, Milagros; Servicio de Endocrinología, Hospital Nacional Cayetano Heredia. Facultad de Medicina Alberto Hurtado, Universidad Peruana Cayetano Heredia. Lima.; Villena Chávez, Jaime; Servicio de Endocrinología, Hospital Nacional Cayetano Heredia. Facultad de Medicina Alberto Hurtado, Universidad Peruana Cayetano Heredia. Lima.

    2012-01-01

    Se describe el caso de un varón de 65 años de edad, sin antecedentes patológicos de importancia, que acudió a emergencia del Hospital Nacional Cayetano Heredia por presentar cefalea intensa y ptosis palpebral izquierda. El examen físico mostró parálisis aislada del III par craneal izquierdo. Los análisis de laboratorio mostraron hiponatremia e hipopituitarismo y la resonancia magnética nuclear un adenoma pituitario con área...

  2. Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles

    OpenAIRE

    Bharat, Tanmay A. M.; Murshudov, Garib N.; Sachse, Carsten; Löwe, Jan

    2015-01-01

    Active segregation of E. coli low-copy number plasmid R1 involves formation of a bipolar spindle made of left-handed double-helical actin-like ParM filaments 1-6 . ParR links the filaments with centromeric parC plasmid DNA, while facilitating the addition of subunits to ParM filaments 3,7-9 . Growing ParMRC spindles push sister plasmids to the cell poles 9,10 . Here, using modern electron cryomicroscopy methods we have investigated the structures and arrangements of ParM filaments in vitro an...

  3. Moniliophthora roreri (Cif y Par Evans et al. in the crop of cocoa

    Directory of Open Access Journals (Sweden)

    Fernando David Sánchez Mora

    2012-09-01

    Full Text Available More than a century, cacao (Theobroma cacao L. in Ecuador has been an important source of income for farmers and currency for the country, becoming one of the biggest exporters worldwide. Then, gradually lost that status due to the attack of diseases, including moniliasis caused by [Moniliophthora roreri (Cif and Par Evans et al.]. It can cause damage of up to 80% in production. Currently there is little scientific date information on its occurrence, symptoms, etiology, epidemiology, life cycle management or control strategies of that disease, by performing this review both these important topics, bringing with it to this area of knowledge.

  4. Revisión de la efectividad de la fisioterapia en el control postural y mayor perfeccionamiento de las habilidades de comunicación: A propósito del caso de un paciente de 12 años con parálisis cerebral

    OpenAIRE

    Hernando Gómez, Jessica

    2016-01-01

    La parálisis cerebral (PC) incluye un grupo de trastornos del desarrollo del movimiento y la postura con limitación notable de la actividad, consecuencia de una alteración no progresiva en el desarrollo cerebral. Es la causa más común de discapacidad infantil. La lesión neurológica puede surgir en la época prenatal, perinatal-natal o postnatal. Los trastornos motores dominantes de la PC son muy variados, resaltando la heterogeneidad en la clasificación topográfica de las les...

  5. Revisión de la efectividad de la fisioterapia en el control postural y mayor perfeccionamiento de las habilidades de comunicación: A propósito del caso de un paciente de 12 años con parálisis cerebral

    OpenAIRE

    Hernando Gómez, Jessica

    2016-01-01

    La parálisis cerebral (PC) incluye un grupo de trastornos del desarrollo del movimiento y la postura con limitación notable de la actividad, consecuencia de una alteración no progresiva en el desarrollo cerebral. Es la causa más común de discapacidad infantil. La lesión neurológica puede surgir en la época prenatal, perinatal-natal o postnatal. Los trastornos motores dominantes de la PC son muy variados, resaltando la heterogeneidad en la clasificación topográfica de las les...

  6. Análisis de la maloclusión y su relación con la posición de reposo de la cabeza y las funciones orales en un grupo de pacientes con parálisis cerebral comparado con un grupo control.

    OpenAIRE

    Martínez Mihi, Victoria

    2016-01-01

    Una de las manifestaciones orales que más preocupa a las personas con parálisis cerebral y a sus padres es la maloclusión, tanto por motivos estéticos como funcionales. En el desarrollo del macizo craneo-facial, afectando a los maxilares y la disposición de los dientes, juegan un papel muy importate, además de la genética, las fuerzas musculares. Estas, tanto en reposo como en función, pueden alterar el desarrollo y originar la maloclusión. Las fuerzas musculares involucradas en el proceso...

  7. Generación sistemática de un modelo de gestión y control de ensayos de máquinas eléctricas para la determinación de sus parámetros característicos y su implementación en un entorno industrial

    OpenAIRE

    2015-01-01

    Esta Tesis Doctoral constituye una herramienta para la determinación sistemática de parámetros de los modelos que caracterizan las diferentes máquinas electricas presentes en los procesos industriales. El avance de las Tecnologías de la Información y las Comunicaciones (TIC) ha permitido la incorporación de nuevos elementos para el análisis y estudio de las máquinas eléctricas. Estos nuevos elementos se han obtenido mediante un estudio del estado de la técnica con el fin de incorporar a la ...

  8. Den eneste ene - hvordan etniske minoritetsunge i Danmark danner par

    DEFF Research Database (Denmark)

    Singla, Rashmi

    Rapporten handler om etniske unges måde at danne par på. Rapporten er baseret på en større empirisk undersøgelse gennemført af forfatteren i 2003 og er desuden inspireret af forfatterens egen ph.d.-afhandling: "Ungdom, etnicitet og psychosocial intervention" (2000, Københavns Universitet). Rappor...

  9. O gênero da parábola

    National Research Council Canada - National Science Library

    MARCO ANTÔNIO; DOMINGUES SANT’ANNA

    2010-01-01

    Este livro pretende demonstrar que o Novo Testamento bíblico é o contexto da constituição da parábola como gênero literário. Mesmo que essa modalidade seja mencionada em outros universos, temos como proposta mostrar...

  10. Radiative Transfer Code: Application to the calculation of PAR

    Indian Academy of Sciences (India)

    D Emmanuel; D Phillippe; C Malik

    2000-12-01

    The production of carbon in the ocean, the so-called primary production, depends on various physico- biological parameters: the biomass and nutrient amounts in oceans, the salinity and temperature of the water and the light available in the water column. We focus on the visible spectrum of the solar radiation defined as the Photosynthetically Active Radiation (PAR). We developed a model (Chami et al. 1997) to simulate the behavior of the solar beam in the atmosphere and the ocean. We first describe the theoretical basis of the code and the method we used to solve the radiative transfer equation (RTE): the successive orders of scattering (SO). The second part deals with a sensitivity study of the PAR just above and below the sea surface for various atmospheric conditions. In a cloudy sky, we computed a ratio between vector fluxes just above the sea surface and spherical fluxes just beneath the sea surface. When the optical thickness of the cloud increases this ratio remains constant and around 1.29. This parameter is convenient to convert vector flux at the sea surface as retrieved from satellite to PAR. Subsequently, we show how solar radiation as vector flux rather than PAR leads to an underestimate of the primary production up to 40% for extreme cases.

  11. Priapisme induit par la chlorpromazine: A propos de deux cas

    Directory of Open Access Journals (Sweden)

    I. Marrag

    2016-06-01

    Le priapisme veineux est une urgence urologique. Il constitue un des effets secondaires des neuroleptiques parmi les quels la chlorpromazine. Cet effet iatrogène, qui est rare mais grave, doit être connu par les cliniciens afin d’être mieux prévenu pour éviter les séquelles érectiles.

  12. Optical engineering application of modeled photosynthetically active radiation (PAR) for high-speed digital camera dynamic range optimization

    Science.gov (United States)

    Alves, James; Gueymard, Christian A.

    2009-08-01

    As efforts to create accurate yet computationally efficient estimation models for clear-sky photosynthetically active solar radiation (PAR) have succeeded, the range of practical engineering applications where these models can be successfully applied has increased. This paper describes a novel application of the REST2 radiative model (developed by the second author) in optical engineering. The PAR predictions in this application are used to predict the possible range of instantaneous irradiances that could impinge on the image plane of a stationary video camera designed to image license plates on moving vehicles. The overall spectral response of the camera (including lens and optical filters) is similar to the 400-700 nm PAR range, thereby making PAR irradiance (rather than luminance) predictions most suitable for this application. The accuracy of the REST2 irradiance predictions for horizontal surfaces, coupled with another radiative model to obtain irradiances on vertical surfaces, and to standard optical image formation models, enable setting the dynamic range controls of the camera to ensure that the license plate images are legible (unsaturated with adequate contrast) regardless of the time of day, sky condition, or vehicle speed. A brief description of how these radiative models are utilized as part of the camera control algorithm is provided. Several comparisons of the irradiance predictions derived from the radiative model versus actual PAR measurements under varying sky conditions with three Licor sensors (one horizontal and two vertical) have been made and showed good agreement. Various camera-to-plate geometries and compass headings have been considered in these comparisons. Time-lapse sequences of license plate images taken with the camera under various sky conditions over a 30-day period are also analyzed. They demonstrate the success of the approach at creating legible plate images under highly variable lighting, which is the main goal of this

  13. Promiscuous stimulation of ParF protein polymerization by heterogeneous centromere binding factors.

    Science.gov (United States)

    Machón, Cristina; Fothergill, Timothy J G; Barillà, Daniela; Hayes, Finbarr

    2007-11-16

    The segrosome is the nucleoprotein complex that mediates accurate segregation of bacterial plasmids. The segrosome of plasmid TP228 comprises ParF and ParG proteins that assemble on the parH centromere. ParF, which exemplifies one clade of the ubiquitous ParA superfamily of segregation proteins, polymerizes extensively in response to ATP binding. Polymerization is modulated by the ParG centromere binding factor (CBF). The segrosomes of plasmids pTAR, pVT745 and pB171 include ParA homologues of the ParF subgroup, as well as diverse homodimeric CBFs with no primary sequence similarity to ParG, or each other. Centromere binding by these analogues is largely specific. Here, we establish that the ParF homologues of pTAR and pB171 filament modestly with ATP, and that nucleotide hydrolysis is not required for this polymerization, which is more prodigious when the cognate CBF is also present. By contrast, the ParF homologue of plasmid pVT745 did not respond appreciably to ATP alone, but polymerized extensively in the presence of both its cognate CBF and ATP. The co-factors also stimulated nucleotide-independent polymerization of cognate ParF proteins. Moreover, apart from the CBF of pTAR, the disparate ParG analogues promoted polymerization of non-cognate ParF proteins suggesting that filamentation of the ParF proteins is enhanced by a common mechanism. Like ParG, the co-factors may be modular, possessing a centromere-specific interaction domain linked to a flexible region containing determinants that promiscuously stimulate ParF polymerization. The CBFs appear to function as bacterial analogues of formins, microtubule-associated proteins or related ancillary factors that regulate eucaryotic cytoskeletal dynamics.

  14. Competing ParA structures space bacterial plasmids equally over the nucleoid.

    Directory of Open Access Journals (Sweden)

    Robert Ietswaart

    2014-12-01

    Full Text Available Low copy number plasmids in bacteria require segregation for stable inheritance through cell division. This is often achieved by a parABC locus, comprising an ATPase ParA, DNA-binding protein ParB and a parC region, encoding ParB-binding sites. These minimal components space plasmids equally over the nucleoid, yet the underlying mechanism is not understood. Here we investigate a model where ParA-ATP can dynamically associate to the nucleoid and is hydrolyzed by plasmid-associated ParB, thereby creating nucleoid-bound, self-organizing ParA concentration gradients. We show mathematically that differences between competing ParA concentrations on either side of a plasmid can specify regular plasmid positioning. Such positioning can be achieved regardless of the exact mechanism of plasmid movement, including plasmid diffusion with ParA-mediated immobilization or directed plasmid motion induced by ParB/parC-stimulated ParA structure disassembly. However, we find experimentally that parABC from Escherichia coli plasmid pB171 increases plasmid mobility, inconsistent with diffusion/immobilization. Instead our observations favor directed plasmid motion. Our model predicts less oscillatory ParA dynamics than previously believed, a prediction we verify experimentally. We also show that ParA localization and plasmid positioning depend on the underlying nucleoid morphology, indicating that the chromosomal architecture constrains ParA structure formation. Our directed motion model unifies previously contradictory models for plasmid segregation and provides a robust mechanistic basis for self-organized plasmid spacing that may be widely applicable.

  15. PH motifs in PAR1&2 endow breast cancer growth

    Science.gov (United States)

    Kancharla, A.; Maoz, M.; Jaber, M.; Agranovich, D.; Peretz, T.; Grisaru-Granovsky, S.; Uziely, B.; Bar-Shavit, R.

    2015-01-01

    Although emerging roles of protease-activated receptor1&2 (PAR1&2) in cancer are recognized, their underlying signalling events are poorly understood. Here we show signal-binding motifs in PAR1&2 that are critical for breast cancer growth. This occurs via the association of the pleckstrin homology (PH) domain with Akt/PKB as a key signalling event of PARs. Other PH-domain signal-proteins such as Etk/Bmx and Vav3 also associate with PAR1 and PAR2 through their PH domains. PAR1 and PAR2 bind with priority to Etk/Bmx. A point mutation in PAR2, H349A, but not in R352A, abrogates PH-protein association and is sufficient to markedly reduce PAR2-instigated breast tumour growth in vivo and placental extravillous trophoblast (EVT) invasion in vitro. Similarly, the PAR1 mutant hPar1-7A, which is unable to bind the PH domain, reduces mammary tumours and EVT invasion, endowing these motifs with physiological significance and underscoring the importance of these previously unknown PAR1 and PAR2 PH-domain-binding motifs in both pathological and physiological invasion processes. PMID:26600192

  16. Modelagem matemática da secagem da pimenta Cumari do Pará Mathematical modeling of drying kinetics of pepper Cumarí do Pará

    Directory of Open Access Journals (Sweden)

    Renata C. Reis

    2011-04-01

    Full Text Available Objetivou-se, neste trabalho, determinar e modelar as curvas de secagem da pimenta Cumari do Pará (Capsicum chinense Jacqui, nas temperaturas de 45, 55 e 65 ºC e estimar seu coeficiente de difusão. As pimentas foram coletadas no município de Ceres, GO, e transportadas para UEG - Anápolis, GO; a secagem foi conduzida em secador experimental com controle de temperatura e fluxo de ar de 60 m³ min-1 m-2; os dados experimentais foram ajustados aos nove modelos matemáticos; as magnitudes do coeficiente de determinação (R², do erro médio relativo (P e do erro médio estimado (SE foram utilizadas para verificar o grau de ajuste dos modelos. Concluiu-se que: a o comportamento das curvas de secagem da pimenta Cumari do Pará foi semelhante ao da maioria dos produtos agrícolas; b o tempo de secagem foi de 4 h; 2 h e 50 min e 1 h e 55 min para as temperaturas de secagem de 45, 55 e 65 ºC, respectivamente; c o modelo matemático de secagem de Midilli et al. (2002, foi o que melhor se ajustou aos dados experimentais; d os valores do coeficiente de difusão aumentaram de acordo com a elevação da temperatura do ar de secagem, apresentando resultados entre 2,29 10-11 e 2,57 10-11 m² s-1.Among the processes of food preservation, drying the product stands out for maintaining the nutritional quality and flavor of products. The objective of this study was to determine and adjust the curves of drying of Pará Cumari pepper (Capsicum chinense Jacqui, at temperatures of 45, 55 and 65 ºC. The Pará Cumari peppers were collected in the city of Ceres, GO and transported to UEG - Anápolis, GO. Drying was conducted in fixed bed dryer with temperature control and airflow of 60 m³ min-1 m-2. Experimental data were adjusted to nine mathematical models. The magnitude of the coefficient of determination (R², the mean relative error (P and the estimated mean error (SE were used to verify the degree of fit of the models. It was concluded that: a the behavior of

  17. Deposition of thin layers of boron nitrides and hydrogenated microcrystalline silicon assisted by high current direct current arc plasma; Deposition assistee par un plasma a arc a haut courant continu de couches minces de Nitrure de Bore et de Silicium microcristallin hydrogene

    Energy Technology Data Exchange (ETDEWEB)

    Franz, D. [Ecole Polytechnique Federale de Lausanne, Centre de Recherches en Physique des Plasmas (CRPP), CH-1015 Lausanne (Switzerland)

    1999-09-01

    In the frame of this thesis, a high current direct current arc (HCDCA) used for the industrial deposition of diamond, has been adapted to study the deposition of two types of coatings: a) boron nitride, whose cubic phase is similar to diamond, for tribological applications, b) hydrogenated microcrystalline silicon, for applications in the semiconductor fields (flat panel displays, solar cells,...). For the deposition of these coatings, the substrates were placed in the diffusion region of the arc. The substrate heating is mainly due to atomic species recombining on its surface. The deposition temperature, varying from 300 to 900 {sup o}C according to the films deposited, is determined by the substrate position, the arc power and the injected gas fluxes, without the use of any external heating or cooling system. Measurements performed on the arc plasma show that the electronic temperature is around 2 eV (23'000 K) while the gas temperature is lower than 5500 K. Typical electronic densities are in the range of 10{sup 12}-10{sup 1'}3 cm{sup -3}. For the deposition of boron nitride films, different boron precursors were used and a wide parameter range was investigated. The extreme difficulty of synthesising cubic boron nitride films by chemical vapour deposition (CVD) did not allow to stabilize the cubic phase of boron nitride in HCDCA. Coatings resulted in hexagonal or amorphous boron nitride with a chemical composition close to stoichiometric. The presence of hydrogen leads to the deposition of rough and porous films. Negative biasing of the samples, for positive ion bombardment, is commonly used to stabilize the cubic phase. In HCDCA and in our biasing range, only a densification of the films could be observed. A boron nitride deposition plasma study by infrared absorption spectroscopy in a capacitive radio frequency reactor has demonstrated the usefulness of this diagnostic for the understanding of the various chemical reactions which occur in this kind of plasma. Diborane dissociation, the different reactions and new species created in the gas phase, such as aminoborane, containing boron-nitrogen bonds, reveals a rich chemistry in the plasma phase. A large amount of atomic hydrogen is necessary to deposit hydrogenated microcrystalline silicon coatings. The study of HCDCA regarding the deposition of diamond has shown that this arc efficiently dissociates molecular hydrogen by way of intermediate vibrationally excited states. This efficient dissociation has allowed the fast deposition (up to 0.6 microns per minute) of highly crystalline silicon films, even without any addition of molecular hydrogen, where the atomic hydrogen was created by the molecular dissociation of silane (SiH{sub 4}). The nature of the deposition precursors is highly dependent on the magnetic field used to confine the arc. At low field, the deposition of hydrogenated silicon radicals created in the plasma leads to columnar growth, whereas with an increase of the magnetic field, the film growth is probably dominated by the deposition of crystallites created in the plasma phase, whose size increases with the field. Moreover, a strong dependence of the film morphology on the deposition parameters has been observed, in particular, regarding the substrate temperature, the hydrogen dilution and the magnetic field. This new deposition technique of hydrogenated microcrystalline silicon is promising for industrial applications. (author)

  18. Theoretical calculation of losses due to coupling currents inside a Nb{sub 3}Sn ``tin inside``-type wire; Calcul theorique des pertes par courants de couplage a l`interieur d`un brin Nb{sub 3}Sn de type ``etain interne``

    Energy Technology Data Exchange (ETDEWEB)

    Otmani, R

    1998-03-01

    The Nb{sub 3}Sn ITER-type wire is made up of 19 sub-elements and 162 filaments compose each sub-element. The calculation of the loss by coupling current inside a wire follows 2 stages: first we evaluate the loss due to the mutual interaction between filaments inside a sub-element then we calculate the loss due to the coupling between sub-elements of the wire. In both calculations the hypothesis of stationary states is made and we assume that the filaments are not saturated. (A.C.) 9 refs.

  19. Detection of metal defects on gas distribution pipeline by remote field eddy current (RFEC) using finite-element analysis; Analyse par la methode des elements finis de la detection des defauts metalliques d'un gazoduc en utilisant les courants de Foucault en champ lointain

    Energy Technology Data Exchange (ETDEWEB)

    Yang, J.H. [R and D Center, Seoul City Gas Co., Ltd. (Korea, Republic of); Yoon, Y.S. [Dept. of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Taejun (Korea, Republic of)

    2001-07-01

    It is necessary to find out whether there are metal defects on underground gas distribution pipelines without excavation in order to establish safety strategies for replacement or maintenance. The metal defects are classified into general corrosion, stress corrosion cracking, lamination, pits, and metal loss, which cause leak or partial damages to a gas pipeline. Therefore, it is required to develop an effective method in the form of an in-line inspection concept that could be implemented internally into a gas pipeline. In this study, theoretical formulations of the magnetic vector potential and magnetic flux density including axial and radial wave numbers based on Maxwell equations are presented analytically to find out the changes of amplitude and phase in the magnetic flux density and the consequent induced voltage which can be a criterion in the remote field eddy current principle for detecting the metal defects in gas pipelines. Three-dimensional finite-element analysis is also presented to analyze the physical phenomenon in metal defects according to each defect size, excitation frequency and moving velocity, which can overcome inaccuracy of the two-dimensional approach, using axisymmetry condition, and simulate local pit conditions occurred severely in real gas pipelines; otherwise only metal loss such as whole circumferential decrease in wall thickness can be modeled. Some experimental works are performed to validate the analytical and finite elemental results regarding the magnetic flux density and induced voltage in the detector. (author)

  20. Characterization of semi-insulating materials by photoinduced current transient spectroscopy: Fe doped INP for micro-optoelectronics and CdZnTe for nuclear detection; Caracterisation de materiaux semi-isolants par spectroscopie de transitoirede courant photoinduit: materiaux INP dopes Fe pour la micro-optoelectronique et CDZNTE pour la detection nucleaire

    Energy Technology Data Exchange (ETDEWEB)

    Cherkaoui, K

    1998-07-01

    The need of semi-insulating materials, of great quality, concerns various application domains. For instance, the very resistive substrates InP and CdZnTe are respectively adapted to the micro-optoelectronic circuits and to nuclear detectors. These two materials have been characterized by the thermal photoinduced current transient spectroscopy. The first part of this thesis is the defects analysis of annealing InP substrates, to understand the compensation process of this material. Two activation energy levels around 0,2 to 0,4 eV resulting from the thermal treatment have been detected. The iron omnipresence in the substrates, even undoped, has been noticed. It is then necessary to take into account the iron presence to understand the compensation process in these InP annealing substrates. the second part presents the study of the CdZnTe material, elaborated by the Bridgman method, to emphasize the defects leading to the decrease of the detector performances. The presence of three deep levels, near the forbidden band middle, is in relation with the detectors performances. (A.L.B.)

  1. Contamination des eaux de puits traditionnels par les nitrates sur le ...

    African Journals Online (AJOL)

    SARAH

    30 juin 2014 ... Objectif : La contamination des eaux souterraines par les nitrates ... Methodology and Results: the origin of nitrate water from traditional wells of Lobo's watershed was ..... avec celles faites par Hudak (1999) au Texas et Liu et.

  2. How to Understand Descartes's Morale par Provision%如何理解笛卡尔的“Morale par Provision”?

    Institute of Scientific and Technical Information of China (English)

    施璇

    2016-01-01

    笛卡尔在《谈谈方法》一书的第三部分中提出了一套道德准则,即morale par provision.最近,一些笛卡尔哲学的研究者们对这一概念提出了三种不同的解读.第一种解读是传统且主流的解读,主张将morale par provision理解为“临时的道德”,它是笛卡尔为了保障自己或他人在贯彻“普遍的方法”的第一步“怀疑的方法”或“方法论怀疑”时所采取的一种权宜之计.第二种解读是将morale par provision理解为“先决的道德”,强调这种道德的先决性与无条件性.第三种对morale par provision的解读是将之理解为“完美道德的一阶近似”.笛卡尔在《谈谈方法》一书中所做的“建筑的比喻”十分清楚地表明了他提出morale par provision的用意及其真正的含义,他将morale par provision比作为了实施房子重建计划而预先准备的临时的房子.因此,Morale par provision其实是笛卡尔为了保障自己或他人在贯彻“普遍的方法”的第一条准则时仍能够不影响日常生活所采纳的一种策略.在这个意义来说,将morale par provision译作“临时的道德”是合适的,换句话说,三种解读中的第一种解读有其合理的依据.《谈谈方法》是morale par provision的出处,因此它无疑是对这一表述进行解释所要依据的最重要的文本.另外两种解读尽管有其合理的地方且具有创新性,但是,只要它们无法强力地否定《谈谈方法》的文本证据,那么它们就无法真正地驳倒第一种解读.此外,关于morale par provision的另外两种解读的支持理由与证据也并非无懈可击.

  3. Preliminary analysis of modeling of Pars and steam injectors to support long-term operation of LWR passive ECCS using a best estimate thermal-hydraulics code

    Energy Technology Data Exchange (ETDEWEB)

    Morales S, J. B.; Sanchez J, J. [UNAM, Facultad de Ingenieria, Circuito Interior s/n, Ciudad Universitaria, 04510 Mexico D. F. (Mexico); Espinosa P, G., E-mail: jaimebmoraless@gmail.co [Universidad Autonoma Metropolitana, Unidad Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, 09340 Mexico D. F. (Mexico)

    2010-10-15

    In order to control the amount of hydrogen produced, during the chemical reaction of steam and strongly overheated zirconium in a LWR severe accident, several control methos have been proposed. In a considerable number of nuclear power plants the initial preferred solution was either the use of an inert containment atmosphere or the use of igniters. However, the use of Pars (Passive autocatalytic recombiners) has been considered an important addition to this set of H{sub 2} controls. Pars have appealing features such as, low maintenance, do not require active components to start operation, and can initiate the H{sub 2} and O{sub 2} recombination at low concentrations. However, under certain conditions these Pars may trigger H{sub 2} ignition leading to deflagration s with possible containment damages. Combustions, poisons and liquid may inhibit or reduce the Pars operation. Advanced LWR designs (generation III + and IV) are also considering the use of Pars as a supporting system to the long term operation of their passive ECCS (Emergency Core Cooling Systems) because they may transport the energy of containment H{sub 2} and O{sub 2} gases to external sinks. Even when the ECCS of modern nuclear power plants are designed to maintain containment conditions under established limits, there are small amounts of O{sub 2} and H{sub 2} produced mainly by H{sub 2}O radiolysis, and after several days of containment isolation, these gases may accumulate in places where the possibility of a H{sub 2} deflagration increases. Therefore, the use of Pars looks initially as an interesting solution to long-term H{sub 2} control during a Dba. However, the H{sub 2} and O{sub 2} recombination is an exothermic process resulting in additional steam and energy releases to the containment. In order to avoid a direct deposition of the steam and energy (Pars produced) into the containment atmosphere, we propose to use them to operate steam injectors that can bring cold water to replenish ECCS

  4. Apoplejía pituitaria con parálisis del III par craneal: Reporte de caso

    Directory of Open Access Journals (Sweden)

    Miguel Pinto Valdivia

    2011-10-01

    Full Text Available Se describe el caso de un varón de 65 años de edad, sin antecedentes patológicos de importancia, que acudió a emergencia del Hospital Nacional Cayetano Heredia por presentar cefalea intensa y ptosis palpebral izquierda. El examen físico mostró parálisis aislada del III par craneal izquierdo. Los análisis de laboratorio mostraron hiponatremia e hipopituitarismo y la resonancia magnética nuclear un adenoma pituitario con áreas de hemorragia e invasión de los senos cavernosos. El tratamiento incluyó glucocorticoides y descompresión quirúrgica transesfenoidal. La anatomía patológica confirmó el diagnóstico de infarto hemorrágico de un adenoma pituitario. El paciente fue dado de alta con terapia sustitutiva de levotiroxina y prednisona. La ptosis palpebral izquierda se recuperó en forma parcial. La apoplejía pituitaria es un síndrome clínico producido por un proceso expansivo dentro de la silla turca, secundario a hemorragia o infarto de un adenoma pituitario, que se caracteriza por cefalea, déficit visual, oftalmoplejía y alteración del nivel de conciencia. Este proceso expansivo puede comprimir los pares craneales en los senos cavernosos, produciendo diferentes grados de parálisis de los músculos oculomotores. La parálisis aislada del III par craneal es rara.(Rev Med Hered 2011;22:186-189.

  5. ParCAT: A Parallel Climate Analysis Toolkit

    Science.gov (United States)

    Haugen, B.; Smith, B.; Steed, C.; Ricciuto, D. M.; Thornton, P. E.; Shipman, G.

    2012-12-01

    Climate science has employed increasingly complex models and simulations to analyze the past and predict the future of our climate. The size and dimensionality of climate simulation data has been growing with the complexity of the models. This growth in data is creating a widening gap between the data being produced and the tools necessary to analyze large, high dimensional data sets. With single run data sets increasing into 10's, 100's and even 1000's of gigabytes, parallel computing tools are becoming a necessity in order to analyze and compare climate simulation data. The Parallel Climate Analysis Toolkit (ParCAT) provides basic tools that efficiently use parallel computing techniques to narrow the gap between data set size and analysis tools. ParCAT was created as a collaborative effort between climate scientists and computer scientists in order to provide efficient parallel implementations of the computing tools that are of use to climate scientists. Some of the basic functionalities included in the toolkit are the ability to compute spatio-temporal means and variances, differences between two runs and histograms of the values in a data set. ParCAT is designed to facilitate the "heavy lifting" that is required for large, multidimensional data sets. The toolkit does not focus on performing the final visualizations and presentation of results but rather, reducing large data sets to smaller, more manageable summaries. The output from ParCAT is provided in commonly used file formats (NetCDF, CSV, ASCII) to allow for simple integration with other tools. The toolkit is currently implemented as a command line utility, but will likely also provide a C library for developers interested in tighter software integration. Elements of the toolkit are already being incorporated into projects such as UV-CDAT and CMDX. There is also an effort underway to implement portions of the CCSM Land Model Diagnostics package using ParCAT in conjunction with Python and gnuplot. Par

  6. Evangelizar em mundo plural: paróquias evangelizadoras?

    Directory of Open Access Journals (Sweden)

    Cleto Caliman

    2009-05-01

    Full Text Available Resumo A missão da Igreja é evangelizar: anunciar ao mundo a boa-nova do Reino de Deus, levando adiante a missão de Jesus. O presente artigo parte da pergunta: em que condições, no mundo pluralista de hoje, a paróquia é uma estrutura que ajuda a evangelização? O mundo em que vivemos não é mais homogêneo, como era o da cristandade. Ele é pluralista. Temos que contar com ele no século XXI. Pretendemos focalizar, especificamente, a paróquia, confrontando-a com a nossa pergunta: ela tem condições de ser evangelizadora? A resposta não pode ser simples. Ela está em algumas teses: cinco são sobre a história da paróquia. Outras cinco sobre as condições para que a paróquia atual seja evangelizadora. O autor finaliza enunciando algumas alternativas à paróquia tradicional, a fim de que ela continue a ser, de modo criativo, um instrumento de evangelização, deixando de ser simplesmente territorial, administrativa, clerical, para ser um laboratório de rede de comunidades, grupos, movimentos. Palavras-chave: Paróquia; Evangelização; Pluralismo.Abstract The Church mission is to evangelize: to announce to the world the good news of God's Kingdom, carrying out Jesus' mission. This article starts with a question: under what conditions, in today's pluralist world, is the parish a structure that fosters evangelization? The world we live in is no longer homogeneous, as that of Chrsitinanity. It is pluralist. We must count on it in the 21st century. . The article focuses specifically on the parish, confronting it with our question: does it have the conditions to be an evangelizing agent? The answer cannot be simple. It is contained in some theses: five about the history of the parish, and another five on the conditions for current parishes to be capable of evangelizing. The author finishes by proposing some alternatives to the traditional parish, so that it may continue to be, in a creative way, an instrument of evangelization: no

  7. The rejection of the comprehensive nuclear test ban treaty by the US Senate: a reverse for the nuclear arms control?; Le rejet du traite d'interdiction complete des essais nucleaires par le Senat Americain: un revers pour l'arms control nucleaire?

    Energy Technology Data Exchange (ETDEWEB)

    Sitt, B. [Universite de Marne la Vallee, 77 (France)

    2000-07-01

    On October 13, 1999, after a hasty debate, the US Senate rejected the comprehensive nuclear test ban treaty (CTBT) signed 3 years ago. This article analyses this event with respect to the US domestic context (discussions at the Senate, reaction of the Presidency) and with respect to the international context (international reactions, future of the treaty, consequences on arms control policy). (J.S.)

  8. Protective effect of a PAR2-activating peptide on histamine-induced bronchoconstriction in guinea-pig

    OpenAIRE

    Cicala, C; Spina, D.; Keir, S D; Severino, B.; Meli, R; Page, C. P.; Cirino, G.

    2001-01-01

    Protease activated receptor-2 (PAR2) is a seven transmembrane domain G protein coupled receptor proteolytically activated. PAR2, together with other PARs, can be also activated by peptides mimicking the sequence of the receptor tethered ligand. We have evaluated the effect of systemic administration of a peptide activating PAR2 (PAR2-AP, SLIGRL) on histamine-induced increase in lung resistances in the guinea-pig.Intravenous administration of PAR2-AP (1 mg kg−1) significantly inhibited histami...

  9. The urokinase receptor (uPAR) facilitates clearance of Borrelia burgdorferi

    NARCIS (Netherlands)

    Hovius, J.W.R.; Bijlsma, M.F.; van der Windt, G.J.W.; Wiersinga, W.J.; Boukens, B.J.D.; Coumou, J.; Oei, A.; de Beer, R.; de Vos, A.F.; van 't Veer, C.; van Dam, A.P.; Wang, P.; Fikrig, E.; Levi, M.M.; Roelofs, J.J.T.H.; van der Poll, T.

    2009-01-01

    The causative agent of Lyme borreliosis, the spirochete Borrelia burgdorferi, has been shown to induce expression of the urokinase receptor (uPAR); however, the role of uPAR in the immune response against Borrelia has never been investigated. uPAR not only acts as a proteinase receptor, but can also

  10. Is There an "F" in Your PAR? Understanding, Teaching and Doing Action Research

    Science.gov (United States)

    Lorenzetti, Liza; Walsh, Christine Ann

    2014-01-01

    Participatory Action Research (PAR) is increasingly recognized within academic research and pedagogy. What are the benefits of including feminism within participatory action research and teaching? In responding to this question, we discuss the similarities and salient differences between PAR and feminist informed PAR (FPAR). There are eight themes…

  11. Five-Port Combined Limbal and Pars Plana Vitrectomy for Infectious Endophthalmitis

    Directory of Open Access Journals (Sweden)

    Kunyong  Xu

    2016-12-01

    Full Text Available Pars plana vitrectomy for acute infectious endophthalmitis can be challenging due to severe inflammation in the anterior chamber creating significant media opacity. We describe a surgical technique combining limbal based vitrectomy and pars plana vitrectomy to manage acute infectious endophthalmitis. Limbal based vitrectomy facilitates removal of anterior chamber fibrin and inflammatory membranes for safe and optimal posterior pars plana vitrectomy.

  12. First (18)F-labeled ligand for PET imaging of uPAR

    DEFF Research Database (Denmark)

    Persson, Morten; Liu, Hongguang; Madsen, Jacob

    2013-01-01

    Urokinase-type plasminogen activator receptor (uPAR) is overexpressed in human prostate cancer and uPAR has been found to be associated with metastatic disease and poor prognosis. AE105 is a small linear peptide with high binding affinity to uPAR. We synthesized an N-terminal NOTA-conjugated vers...

  13. Artisan fishing and the fishing company in Óbidos, Pará

    Directory of Open Access Journals (Sweden)

    Adriana de Aviz

    2006-08-01

    Full Text Available This work is product of the Project "The time of the plant: the disciplinamento of the workers of the fishing industry (Óbidos-Pará: a comparative study", developed in the years of 2002 and 2003 in the municipality of Óbidos, Pará, objectifying to understand the importance of the time in the disciplinamento of the work in the obidense fishing company. Interviews opened in entrepreneurs had been carried through of fish, controlling, heads of quality control, people in charge of hall and other actors involved in the sector fishing boat and applied half-open questionnaires with laborers and fishing. One used the photographic, recording register and the notebook of notations. The research was carried through in the fishing companies Mundial and Pasquarelli, Colony Z-19 Fishing, in the boats (river Amazon of the artisan fishing and in its residences. As central focus it was looked for to unmask the characteristics of the 'disciplined time' in the sector of improvement of the fishing company, and to detach the joint of the 'natural time' in the process of capture of the fished one. Leaving of these estimated, it was used the 'disciplined time' and the 'natural time' conceived by Thompson (1975.

  14. Caractérisation du dipôle lacune-calcium par relaxations électrique et mécanique dans des mononcristaux de zircones calciées

    Science.gov (United States)

    Pautrot, S.; Mazot, Ph.; de Fouquet, J.

    1993-05-01

    Complex impedance measurements have been performed on calcia doped zirconia single crystals for three different dopant concentrations. A maximum of the dielectric loss is observed at a frequency which is thermally activated. The activation energy is slightly lower than the one derived from the study of the dc electrical conductivity with temperature. The experimental results depend very little on composition. Resonant method experiments have been used to measure internal friction and Young's modulus for different orientations [100], [110] and [111] in he case of one composition. The study with temperature confirms that [111] is the preferential orientation of the vacancy-calcium dipole. Des mesures isothermes d'impédance complexe ont été réalisées sur des échantillons monocristallins de zircone cuhique stabilisée à la chaux, pour trois compositions différentes. L'étude de la conductivité en courant continu conduit à la détermination d'une énergie d'activation légèrement superieure à celle obtenue lors de l'étude en fréquence de l'angle de pertes diélectriques. Pour une concentration donnée, nous avons également mesuré, par une méthode de résonance, le frottement intérieur ainsi que le module d'Young d'un échantillon selon les trois orientations cristallographiques [100], [111] et [110]. Cette étude confirme la direction du dipôle lacune-calcium selon un axe [111].

  15. Assistance to a car heat engine by a self-controlled synchronous machine: engine energy control enhancement and pollutant emission reduction; Assistance d`un moteur thermique d`automobile par une machine synchrone autopilotee: amelioration de la gestion energetique et reduction des polluants du groupe moto-propulseur

    Energy Technology Data Exchange (ETDEWEB)

    Kouadio, L.

    1996-12-12

    A new method for spark-ignition engine control, more especially in idle conditions, has been developed to prevent engine stalling and improve its idling performances in terms of fuel consumption and pollutant emissions: the car synchronous machine, traditionally operating as an alternator, is used in certain phases to provide an external supplementary torque on the engine shaft. Methods are proposed for optimising the synchronous machine operation as a generator and as an engine, then the main engine control parameters are presented and a linear signal model is established around a nominal idling point, which is used for simulations. Experimental results show evident reductions of fuel consumption and a significant robustness improvement in the engine dynamics

  16. Control del parásito Varroa destructor (Acari: Varroidae en colmenas de la abeja Apis mellifera (Hymenoptera: Apidae mediante la aplicación de la técnica de entrampado Control of the parasite Varroa destructor (Acari: Varroidae in honeybee colonies of Apis mellifera (Hymenoptera: Apidae applying brood trap combs

    Directory of Open Access Journals (Sweden)

    Natalia Damiani

    2006-07-01

    Full Text Available La parasitosis causada por el ácaro Varroa destructor (Anderson & Trueman es, actualmente considerada el mayor escollo para el desarrollo de la apicultura. El objetivo del presente trabajo fue evaluar la técnica del entrampamiento de ácaros en panales de cría, como posible método de control de la parasitosis. El trabajo se llevó a cabo en Coronel Vidal, provincia de Buenos Aires. Se trabajó sobre colmenas tipo Langstroth del híbrido regional de Apis mellifera (Linneaus. En cada colmena experimental se procedió a confinar a la reina en panales trampa específicos, con el fin de poder controlar la oviposición. Estos panales, luego de ser operculados por las obreras, fueron llevados al laboratorio donde se desoperculó cada una de las celdas de cría, y se contabilizó el número de ácaros presentes. Esta técnica se aplicó variando el número de panales trampa (1-3 colocados, tanto para los constituidos por celdas de cría de obreras como de zánganos. También, se evaluó el impacto de la aplicación de esta técnica sobre el desarrollo de las colonias, mediante la medición de su productividad. Los resultados indican, que la técnica empleada sólo es efectiva, cuando se aplican tres panales de cría de zánganos de manera consecutiva, alcanzando una efectividad máxima de 84%. Cuando se aplican tres panales de obreras, la técnica mostró niveles de efectividad muy inferiores (14%. En las colonias sobre las que se aplicó esta técnica, la productividad de miel se redujo significativamente, comparada con las colonias control. Esta técnica resulta ideal para ser combinada con otros mecanismos de control, disminuyendo la aplicación de sustancias químicas que puedan contaminar la miel, y la generación de resistencia por parte del ácaro frente a los principios activos utilizados para su control.At present, Varroosis is considered the major problem to beekeeping development. The aim of this work was to evaluate brood tramp combs

  17. Cross-modal representation of spoken and written word meaning in left pars triangularis.

    Science.gov (United States)

    Liuzzi, Antonietta Gabriella; Bruffaerts, Rose; Peeters, Ronald; Adamczuk, Katarzyna; Keuleers, Emmanuel; De Deyne, Simon; Storms, Gerrit; Dupont, Patrick; Vandenberghe, Rik

    2017-04-15

    The correspondence in meaning extracted from written versus spoken input remains to be fully understood neurobiologically. Here, in a total of 38 subjects, the functional anatomy of cross-modal semantic similarity for concrete words was determined based on a dual criterion: First, a voxelwise univariate analysis had to show significant activation during a semantic task (property verification) performed with written and spoken concrete words compared to the perceptually matched control condition. Second, in an independent dataset, in these clusters, the similarity in fMRI response pattern to two distinct entities, one presented as a written and the other as a spoken word, had to correlate with the similarity in meaning between these entities. The left ventral occipitotemporal transition zone and ventromedial temporal cortex, retrosplenial cortex, pars orbitalis bilaterally, and the left pars triangularis were all activated in the univariate contrast. Only the left pars triangularis showed a cross-modal semantic similarity effect. There was no effect of phonological nor orthographic similarity in this region. The cross-modal semantic similarity effect was confirmed by a secondary analysis in the cytoarchitectonically defined BA45. A semantic similarity effect was also present in the ventral occipital regions but only within the visual modality, and in the anterior superior temporal cortex only within the auditory modality. This study provides direct evidence for the coding of word meaning in BA45 and positions its contribution to semantic processing at the confluence of input-modality specific pathways that code for meaning within the respective input modalities. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. Results of Preliminary Tests of PAR Bunch Cleaning

    CERN Document Server

    Yao, Chihyuan; Grelick, Arthur; Lumpkin, Alex H; Sereno, Nicholas S

    2005-01-01

    A particle accumulator ring (PAR) is used at the Advanced Photon Source (APS) to collect multiple linac bunches and compress them into a 0.3-ns (rms) single bunch for booster injection. A 9.77-MHz fundamental rf system and a 117.3-MHz harmonic rf system are employed for initial beam capture and bunch length compression. Satellite bunches with very low charge form due to rf phase drifts or beam loading change. These satellites, when injected into the booster and then into the storage ring (SR), cause bunch impurity at three buckets from the target bucket. Storage ring and booster bunch cleaning was tried but proved to be difficult due to the top-up mode of operation in the storage ring and tune drift in the booster synchrotron. Recently we implemented a PAR bunch-cleaning system with tune-modulated harmonic rf knockout. Preliminary tests gave a measured SR bunch purity of better than 10

  19. Cross ambiguity functions on the MasPar MP-2

    Energy Technology Data Exchange (ETDEWEB)

    Carlson, D.A.; Pryor, D.V. [Superconducting Research Center, Bowie, MD (United States); Frock, C.K. [and others

    1995-12-01

    In a signal processing environment, cross ambiguity functions are often used to detect when one signal is a time and/or frequency shift of another. They consist of multiple cross-correlations, which can be computed efficiently using complex valued FFTs. This paper discusses the implementation of cross ambiguity functions on the MasPar MP-2, a SIMD processor array. Two different implementations are developed. The first computes each cross ambiguity function serially, using FFT code that parallelizes across the complete set of processors. The second uses the MasPar IORAM to realign the data so that the cross ambiguity functions can be computed in parallel. In this case, multiple FFTs are executed in parallel on subsets of the processors, which lowers the overall amount of communication required.

  20. Algorithmique et contrôle en vision par ordinateur

    OpenAIRE

    Lux, Augustin

    1985-01-01

    Cette thèse traite de la Vision par Ordinateur - VO - et de sa relation avec l'Intelligence Artificielle - IA. Elle est composée de trois parties. La partie I "La Vision par Ordinateur - présentation et réflexions" donne, après une définition des deux domaines, un aperçu des approches théoriques et des réalisations existantes en VO utilisant des concepts de l'IA. Nous développons ensuite notre approche qui intégre 1e concept de structures de contrôle au sens de l'IA dans un système algorithmi...

  1. La syphilis congenitale revelee par une fracture spontanee

    Directory of Open Access Journals (Sweden)

    Mounia Lakhdar Idrissi

    2011-11-01

    Full Text Available Alors qu�elle est actuellement oubliee dans les pays developpes, la syphilis congenitale se voit encore chez nous faute du depistage antenatal. Ses formes cliniques sont polymorphes et orientent a tord vers d�autres pathologies surtout en periode neonatale. Le diagnostic n�est donc pas toujours facile. La revelation d�une syphilis congenitale par une fracture spontanee est exceptionnellement decrite. Nous rapportons dans ce travail le cas d�un nourrisson de 2 mois ramene en consultation pour limitation douloureuse des mouvements du bras droit. Le diagnostic est evoque sur les donnees radiologiques et confirme par la serologie syphilitique. Le traitement a repose essentiellement sur l�administration de la penicilline G avec une bonne evolution clinique.

  2. Intraoperative raster photogrammetry--the PAR Corneal Topography System.

    Science.gov (United States)

    Belin, M W

    1993-01-01

    The PAR Corneal Topography System (CTS) is a computer-driven corneal imaging system that uses close-range raster photogrammetry to measure and produce a topographic map of the corneal surface. The CTS determines distortion in a projected two-dimensional grid. Unlike Placido-disc-based videokeratoscopes, the PAR CTS produces a true topographic map (elevation map) and requires neither a smooth reflective surface nor precise spatial alignment for accurate imaging. Because the system uses two noncoaxial optical paths, it can be integrated into other optical devices. A modified CTS was integrated into an experimental erbium: YAG photoablative laser. The CTS successfully imaged corneas before, after, and during laser photoablation. Its ability to image nonreflective surfaces and to be integrated into other optical systems may make it suitable for intraoperative refractive monitoring.

  3. Exploration of a new series of PAR1 antagonists.

    Science.gov (United States)

    Planty, Bruno; Pujol, Chantal; Lamothe, Marie; Maraval, Catherine; Horn, Clemens; Le Grand, Bruno; Perez, Michel

    2010-03-01

    Two series of new PAR1 antagonists have been identified. The first incorporates a cinnamoylpiperidine motif and the second a cinnamoylpyridine pattern. The synthesis, biological activity and structure-activity relationship of these compounds are presented. In each series, one analog showed potent in vivo antithrombotic activity in a rat AV shunt model, with up to 53% inhibition at 1.25mpk iv for compound 30.

  4. Tonic Pupil Following Pars Plana Vitrectomy and Endolaser

    Directory of Open Access Journals (Sweden)

    Benyamin Ebrahim

    2009-01-01

    Full Text Available Tonic pupil was observed in a 67 year-old patient following a retinal detachment repair with pars plana vitrectomy, endolaser and silicone oil tamponade performed under retrobulbar anesthesia. The probable location of disturbance is the postganglionic parasympathetic fibers in the short ciliary nerves along their course to the pupil in the suprachoroidal space. A likely explanation for this phenomenon is injury to short ciliary nerves by endolaser treatment.

  5. La parálisis cerebral en el contexto escolar /

    OpenAIRE

    González Vara, Azucena

    2012-01-01

    Se analiza el concepto de parálisis cerebral, los diferentes tipos y sus características. También las adaptaciones que se deben realizar a nivel de centro, de aula, y a nivel individual para estos alumnos, conocer los diferentes sistemas alternativos y aumentativos de comunicación y aplicar las ayudas técnicas que precisa este alumnado

  6. The 2010 Polar Aeronomy and Radio Science (PARS) Summer School

    Science.gov (United States)

    2011-12-30

    Auroral Research Program ( HAARP ) Research Station at Gakona, Alaska. The 2010 PARS Summer School, conducted over the period July 12 – 22, 2010...included a total of 21 students and 14 advisors or visiting scientists representing 12 universities. During the research period at the HAARP facility... HAARP , Stimulated Electromagnetic Emissions (SEE) 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT 18. NUMBER OF PAGES 19a

  7. Design Studies and Commissioning Plans for PARS Experimental Program

    CERN Document Server

    Mete, O; Xia, G; Dover, M; Wigram, M; Wright, J; Zhang, J; Smith, J

    2015-01-01

    PARS (Plasma Acceleration Research Station) is an electron beam driven plasma wakefield acceleration test stand proposed for VELA/CLARA facility in Daresbury Laboratory. In order to optimise various operational configurations, 2D numerical studies were performed by using VSIM for a range of parameters such as bunch length, radius, plasma density and positioning of the bunches with respect to each other for the two-beam acceleration scheme. In this paper, some of these numerical studies and considered measurement methods are presented.

  8. Accuracy of the PAR corneal topography system with spatial misalignment.

    Science.gov (United States)

    Belin, M W; Zloty, P

    1993-01-01

    The PAR Corneal Topography System is a computerized corneal imaging system which uses close-range raster photogrammetry to measure and produce a topographic map of the corneal surface. Raster photogrammetry is a standard method of extracting object information by projecting a known pattern onto an object and recording the distortion when viewed from an oblique angle. Unlike placido disc based videokeratoscopes, the PAR system requires neither a smooth reflective surface nor precise spatial alignment for accurate imaging. We studied both the accuracy of the system with purposeful misalignment (defocusing) of the test object and determined the ability to image freshly deepithelialized, keratectomized, and photoablated corneas. The PAR system was both accurate and reproducible in imaging calibrated spheres within a defined zone in space. Whole cadaver eyes were imaged both before and immediately after removal of the epithelium, lamellar keratectomy, and laser photoablation. The system demonstrated the ability to image irregular, deepithelialized, and keratectomized corneas. The ability to maintain accuracy without precise alignment and the facility to image freshly deepithelialized and keratectomized corneas may make the system suitable for intraoperative refractive monitoring.

  9. Parâmetros genéticos para a produção de leite de controles individuais de vacas da raça Gir estimados com modelos de repetibilidade e regressão aleatória Estimation of genetic parameters for test day milk records of first lactation Gyr cows using repeatability and random regression animal models

    Directory of Open Access Journals (Sweden)

    Claudio Napolis Costa

    2005-10-01

    Full Text Available Registros individuais perfazendo 8.183 controles de produção de leite (PLC de 1.273 primeiras lactações de vacas da raça Gir de rebanhos supervisionados pela ABCZ no período 1994/2000 foram utilizados para se estimar componentes de variância e parâmetros genéticos para PLC usando-se REML. Foram comparados um modelo de repetibilidade e modelos de regressão aleatória ajustados com as funções logarítmica (Ali & Schaeffer, 1987, exponencial (Wilmink, 1987 e polinômios ortogonais de Legendre (LP de ordem 3 a 5, sob as pressuposições de homogeneidade e heterogeneidade de variância residual, definidas respectivamente por uma (ME=1 ou quatro classes de medidas de erro temporário (ME=4 ao longo do período de lactação. Também ajustou-se a produção de leite acumulada na lactação (P305 utilizando-se um modelo animal cuja estimativa de heritabilidade foi 0,22. As estimativas de heritabilidade e repetibilidade para a PLC foram 0,27 e 0,76, respectivamente. As estimativas de heritabilidade com a FAS e a FW alcançaram respectivamente 0,59 e 0,74 ao início da lactação e decresceram para valores próximos de 0,20 ao final do período. Exceto para o LP de quinta ordem com ME=1, as estimativas de heritabilidade diminuíram de 0,70 no início para 0,30 no final da lactação. Menores estimativas de VR foram obtidas para a FAS do que para a FW sob ambas as pressuposições de homogeneidade e heterogeneidade de variância. Em todos os estádios da lactação, as estimativas de VR diminuíram com o aumento da ordem do LP e dependeram da pressuposição sobre o ME. As estimativas das variâncias genética e de ambiente permanente não apresentaram nenhuma tendência com o aumento da ordem do LP e não se observaram diferenças significativas para tais estimativas sob a pressuposição de heterogeneidade de VR ao longo da lactação. Maiores valores de correlação genética entre as PLC foram obtidos com a FW, que também apresentou maior

  10. L'exraction par micro-ondes: un choix de qualité

    OpenAIRE

    Ramdani, M.; Ghazi, Z.; Fadel, O.; Mouni, L.; Wathelet, Jean-Paul

    2010-01-01

    Le but de ce travail est de comparer l’extraction faite par chauffage classique (CC) avec celle réalisée sous micro-ondes (MO). Deux modes d’extraction ont été réalisés : - L’hydro-distillation par chauffage classique (extraction par entraînement à la vapeur d’un solvant) et, - L’hydro-distillation activée par micro-ondes. Les MO permettent un gain de temps considérable. Par exemple, dans le cas du lavandula dentata : on note une cinétique 3 fois plus rapide, de plus le rendement ...

  11. Estimativas de Parâmetros fenotípicos e ambientes das Produções de Leite no Dia do Controle e em 305 Dias de Lactação em vacas da Raça Gir Estimates of phenotypic and environmental parameters of milk production at the control day and 305 days of lactation in gir breed cows

    Directory of Open Access Journals (Sweden)

    Ivan Luz Ledic

    2003-08-01

    Full Text Available Dados de 32.779 controles mensais, de 3.605 lactações em 305 dias (PL305, de 2.082 vacas Gir, filhas de 281 touros, com partos ocorridos de 1987 a 1999, em 11 rebanhos, foram usados com o objetivo de verificar os fatores que afetam a produção de leite no dia do controle (PLDC em avaliações na raça Gir. Foram realizadas análises univariadas das PLDC1 a PLDC10 e da PL305 pelo método de máxima verossimilhança restrita, sob modelo animal, incluindo as três primeiras lactações como medidas repetidas de um mesmo animal, diferenciado conforme o rebanho, o ano e a estação do parto (PL305 ou do controle (PLDC1 a PLDC10, a idade da vaca ao parto, a ordem do parto e do intervalo parto-primeiro controle (ipc na PLDC1. As médias observadas e os respectivos desvios-padrão (kg para PLDC1 a PLDC10 e PL305 foram: 11,97±4,64; 11,93±4,68; 10,98±4,40; 10,18±4,12; 9,66±3,88; 9,20±3,69; 8,63±3,51; 8,08±3,33; 7,59±3,27; 7,22±3,15 e 2.746,17±1.299,90. A produção máxima ocorreu no 23° dia, conforme o ipc. Ocorreu grande variação em relação ao ano e estação, conforme a característica considerada, sem haver tendência definida. As diferenças entre as produções dos rebanhos foram acima de 50%. Nos primeiros quatro controles, as produções foram muito afetadas pela idade da vaca e as vacas com idade entre 70 a 100 meses apresentaram produções mais elevadas; na PL305, a produção aumentou até 88 meses de idade. As produções de leite das terceiras ordens foram mais altas em relação aos do segundo e primeiro partos, com diferenças acima de 11%.Data of 32,779 monthly milk production, of 3,605 lactations in 305 days (PL305, of 2,082 Gir breed cows, daughters of 281 sires, calving from 1987 to 1999, in 11 herds, were utilized to verify the factors that affect the milk production in the control day (PLDC in evaluations of the Gir breed. Univariates analysis on PLDC1 to PLDC10 and Pl305 were performed utilizing the restricted

  12. ParB Partition Proteins: Complex Formation and Spreading at Bacterial and Plasmid Centromeres

    Directory of Open Access Journals (Sweden)

    Barbara Funnell

    2016-08-01

    Full Text Available In bacteria, active partition systems contribute to the faithful segregation of both chromosomes and low-copy-number plasmids. Each system depends on a site-specific DNA binding protein to recognize and assemble a partition complex at a centromere-like site, commonly called parS. Many plasmid and all chromosomal centromere-binding proteins are dimeric helix-turn-helix DNA binding proteins, which are commonly named ParB. Although the overall sequence conservation among ParBs is not high, the proteins share similar domain and functional organization, and they assemble into similar higher-order complexes. In vivo, ParBs spread; that is, DNA binding extends away from the parS site into the surrounding nonspecific DNA, a feature that reflects higher-order complex assembly. ParBs bridge and pair DNA at parS and nonspecific DNA sites. ParB dimers interact with each other via flexible conformations of an N-terminal region. This review will focus on the properties of the HTH centromere-binding protein, in light of recent experimental evidence and models that are adding to our understanding of how these proteins assemble into large and dynamic partition complexes at and around their specific DNA sites.

  13. Specific and non-specific interactions of ParB with DNA: implications for chromosome segregation.

    Science.gov (United States)

    Taylor, James A; Pastrana, Cesar L; Butterer, Annika; Pernstich, Christian; Gwynn, Emma J; Sobott, Frank; Moreno-Herrero, Fernando; Dillingham, Mark S

    2015-01-01

    The segregation of many bacterial chromosomes is dependent on the interactions of ParB proteins with centromere-like DNA sequences called parS that are located close to the origin of replication. In this work, we have investigated the binding of Bacillus subtilis ParB to DNA in vitro using a variety of biochemical and biophysical techniques. We observe tight and specific binding of a ParB homodimer to the parS sequence. Binding of ParB to non-specific DNA is more complex and displays apparent positive co-operativity that is associated with the formation of larger, poorly defined, nucleoprotein complexes. Experiments with magnetic tweezers demonstrate that non-specific binding leads to DNA condensation that is reversible by protein unbinding or force. The condensed DNA structure is not well ordered and we infer that it is formed by many looping interactions between neighbouring DNA segments. Consistent with this view, ParB is also able to stabilize writhe in single supercoiled DNA molecules and to bridge segments from two different DNA molecules in trans. The experiments provide no evidence for the promotion of non-specific DNA binding and/or condensation events by the presence of parS sequences. The implications of these observations for chromosome segregation are discussed.

  14. Comportamento de baixas doses de herbicidas na cultura da soja (Glycine max (L. Merril: I - efeitos sobre o controle das plantas daninhas e parâmetros de produção da cultura Behavior of low-rates of herbicides in the soybean (Glycine max (L . Merril crop: I - effects on weed control and crop yield parameters

    Directory of Open Access Journals (Sweden)

    J. C. Durigan

    1983-06-01

    Full Text Available Foi estudada a possibilidade de redução nas doses recomendadas de herbicidas, isolados ou em misturas, sem afetar a produção ou outras características desejáveis da planta, para o cultivar Santa Rosa em Solo Latossol Vermelho Escuro -fase arenosa. 0 experimento foi instalado em blocos ao acaso, com vinte tratamentos e três repetições, testando-se a dose total recomendada e reduções de 25% e 50% dela, para trifluralin, alachlor e metribuzin, isolados e em misturas. As doses recomendadas foram 0,86; 1,72 e 0,28 kg/ha de trifluralin, alachlor e metribuzin, respectivamente. As misturas com doses reduzidas, de tri-firalin + metribuzin (0,65 + 0,21 kg/ha e alachlor + metribuzin (1,44 4 0,21 kg/ha, apresentaram controle geral das plantas daninhas acima de 90% até o 60.º dia após a semeadura, sem apresentar fitotoxicidade ou efeitos deletérios sobre a nodulação da soja. O controle das plantas daninhas obtido com a redução de 25% nas doses destas misturas, foi equivalente ao da testemunha capinada e proporcionou valores similares nos teores de proteína, extrato etéreo e cinzas, peso de 100 grãos, número de grãos por vagem, altura da planta e altura da inserção da vagem mais baixa. As maiores produções de soja, obtidas com as misturas na dose total recomendada, foram estatisticamente iguais às obtidas com estes mesmos herbicidas aplicados com dose padrão reduzida em 25%. Desta forma, de acordo com as conveniências econômicas e ecológicas, sugere-se que a recomendação atual destes tratamentos seja feito com as doses reduzidas em 25%, nas condições de desenvolvimento da presente pesquisa.It was studied the feasibility of reducing there commended herbicide rate, single or in mixtures, without affecting the yield or other desirable plant features, for cultivar Santa Rosa in "Dark-Red Latossol -sandy phase". ' The trials was settled at randomized block design with treatments repplicated 3 times, testing the full recommended

  15. Control of radioactive wastes and coupling of neutron/gamma measurements: use of radiative capture for the correction of matrix effects that penalize the fissile mass measurement by active neutron interrogation; Controle des dechets radioactifs et couplage de mesures neutron/gamma: exploitation de la capture radiative pour corriger les effets de matrice penalisant la mesure de la masse fissile par interrogation neutronique active

    Energy Technology Data Exchange (ETDEWEB)

    Loche, F

    2006-10-15

    In the framework of radioactive waste drums control, difficulties arise in the nondestructive measurement of fissile mass ({sup 235}U, {sup 239}Pu..) by Active Neutron Interrogation (ANI), when dealing with matrices containing materials (Cl, H...) influencing the neutron flux. The idea is to use the neutron capture reaction (n,{gamma}) to determine the matrix composition to adjust the ANI calibration coefficient value. This study, dealing with 118 litres, homogeneous drums of density less than 0,4 and composed of chlorinated and/or hydrogenated materials, leads to build abacus linking the {gamma} ray peak areas to the ANI calibration coefficient. Validation assays of these abacus show a very good agreement between the corrected and true fissile masses for hydrogenated matrices (max. relative standard deviation: 23 %) and quite good for chlorinated and hydrogenated matrices (58 %). The developed correction method improves the measured values. It may be extended to 0,45 density, heterogeneous drums. (author)

  16. Effect of PAR-2 Deficiency in Mice on KC Expression after Intratracheal LPS Administration

    Directory of Open Access Journals (Sweden)

    Julie C. Williams

    2011-01-01

    Full Text Available Protease activated receptors (PAR have been shown to play a role in inflammation. PAR-2 is expressed by numerous cells in the lung and has either proinflammatory, anti-inflammatory, or no effect depending on the model. Here, we examined the role of PAR-2 in a model of LPS-induced lung inflammation. We found that PAR-2-deficient mice had significantly less KC expression in bronchial lavage fluid compared with wild-type mice but there was no difference in MIP-2 or TNF-α expression. We also found that isolated alveolar and resident peritoneal macrophages lacking PAR-2 showed a similar deficit in KC after LPS stimulation without differences in MIP-2 or TNF-α. Infiltration of neutrophils and macrophages into the lung following LPS administration was not affected by an absence of PAR-2. Our results support the notion that PAR-2 plays a role in LPS activation of TLR4 signaling in macrophages.

  17. Open Issues in Supervisory Control of Hybrid Electric Vehicles: A Unified Approach Using Optimal Control Methods Questions ouvertes sur la supervision énergétique des véhicules hybrides électriques : une approche unifiée par la théorie de la commande optimale

    Directory of Open Access Journals (Sweden)

    Serrao L.

    2013-03-01

    Full Text Available Energy management of hybrid propulsion systems is considered, presenting new issues that extend the energy management role beyond the standard torque splitting to maximize system efficiency. The new issues include additional optimization criteria, constraints and relevant dynamics to deal with. New optimization criteria in addition the sole fuel consumption minimization include engine-out pollutant emissions and battery aging. State constraints are modified to account for plug-in hybrid vehicles. Moreover, specific supervisory control problems are recognized to need additional state variables. The latter comprise: engine and catalyst temperature to deal with engine warm-up effects on fuel consumption and after-catalyst emissions; thermal dynamics of heat recovery systems (Rankine or Thermo-Electric Generators, TEGs; and battery temperature, which influences battery performance and aging. It is shown that all these control problems can be treated in an unified fashion by extending the well-known ECMS (Equivalent Consumption Minimization Strategy, which is an implementation of Pontryagin Minimum Principle (PMP as formulated by optimal control theory. Extended definitions of the Hamiltonian function and Lagrange multipliers are introduced. Optimization runs performed off line are reported. Results show the benefits of the proposed unified approach and enlighten some first online implementation issues. Cet article a pour objet la gestion optimale de l’énergie pour un système de propulsion hybride. Le problème traditionnel de répartition de la puissance est modifié avec des nouveaux objectifs d’optimisation et des nouvelles contraintes. Les nouveaux objectifs d’optimisation incluent les émissions de polluants et le vieillissement de la batterie. Les contraintes sont modifiées pour prendre en compte des batteries à recharge externe (hybrides plug-in. De plus, des problèmes spécifiques sont traités avec une modélisation plus d

  18. Mammalian aPKC/Par polarity complex mediated regulation of epithelial division orientation and cell fate.

    Science.gov (United States)

    Vorhagen, Susanne; Niessen, Carien M

    2014-11-01

    Oriented cell division is a key regulator of tissue architecture and crucial for morphogenesis and homeostasis. Balanced regulation of proliferation and differentiation is an essential property of tissues not only to drive morphogenesis but also to maintain and restore homeostasis. In many tissues orientation of cell division is coupled to the regulation of differentiation producing daughters with similar (symmetric cell division, SCD) or differential fate (asymmetric cell division, ACD). This allows the organism to generate cell lineage diversity from a small pool of stem and progenitor cells. Division orientation and/or the ratio of ACD/SCD need to be tightly controlled. Loss of orientation or an altered ratio can promote overgrowth, alter tissue architecture and induce aberrant differentiation, and have been linked to morphogenetic diseases, cancer and aging. A key requirement for oriented division is the presence of a polarity axis, which can be established through cell intrinsic and/or extrinsic signals. Polarity proteins translate such internal and external cues to drive polarization. In this review we will focus on the role of the polarity complex aPKC/Par3/Par6 in the regulation of division orientation and cell fate in different mammalian epithelia. We will compare the conserved function of this complex in mitotic spindle orientation and distribution of cell fate determinants and highlight common and differential mechanisms in which this complex is used by tissues to adapt division orientation and cell fate to the specific properties of the epithelium. Copyright © 2014 Elsevier Inc. All rights reserved.

  19. Chronic Rhinosinusitis Patients Show Accumulation of Genetic Variants in PARS2.

    Directory of Open Access Journals (Sweden)

    Viktor Henmyr

    Full Text Available Genetic studies of chronic rhinosinusitis (CRS have identified a total of 53 CRS-associated SNPs that were subsequently evaluated for their reproducibility in a recent study. The rs2873551 SNP in linkage disequilibrium with PARS2 showed the strongest association signal. The present study aims to comprehensively screen for rare variants in PARS2 and evaluate for accumulation of such variants in CRS-patients. Sanger sequencing and long-range PCR were used to screen for rare variants in the putative promoter region and coding sequence of 310 CRS-patients and a total of 21 variants were detected. The mutation spectrum was then compared with data from European populations of the 1000Genomes project (EUR and the Exome Aggregation Consortium (ExAC. The CRS population showed a significant surplus of low-frequency variants compared with ExAC data. Haplotype analysis of the region showed a significant excess of rare haplotypes in the CRS population compared to the EUR population. Two missense mutations were also genotyped in the 310 CRS patients and 372 CRS-negative controls, but no associations with the disease were found. This is the first re-sequencing study in CRS research and also the first study to show an association of rare variants with the disease.

  20. Identification of dopaminergic neurons of the substantia nigra pars compacta as a target of manganese accumulation

    Science.gov (United States)

    Robison, Gregory; Sullivan, Brendan; Cannon, Jason R.; Pushkar, Yulia

    2015-01-01

    Manganese serves as a cofactor to a variety of proteins necessary for proper bodily development and function. However, an overabundance of Mn in the brain can result in manganism, a neurological condition resembling Parkinson’s disease (PD). Bulk sample measurement techniques have identified the globus pallidus and thalamus as targets of Mn accumulation in the brain, however smaller structures/cells cannot be measured. Here, X-ray fluorescence microscopy determined the metal content and distribution in the substantia nigra (SN) of the rodent brain. In vivo retrograde labeling of dopaminergic cells (via FluoroGold™) of the SN pars compacta (SNc) subsequently allowed for XRF imaging of dopaminergic cells in situ at subcellular resolution. Chronic Mn exposure resulted in a significant Mn increase in both the SN pars reticulata (>163%) and the SNc (>170%) as compared to control; no other metal concentrations were significantly changed. Subcellular imaging of dopaminergic cells demonstrated that Mn is located adjacent to the nucleus. Measured intracellular manganese concentrations range between 40–200 μM; concentrations as low as 100 μM have been observed to cause cell death in cell cultures. Direct observation of Mn accumulation in the SNc could establish a biological basis for movement disorders associated with manganism, specifically Mn caused insult to the SNc. Accumulation of Mn in dopaminergic cells of the SNc may help clarify the relationship between Mn and the loss of motor skills associated with manganism. PMID:25695229

  1. Mammalian aPKC/Par polarity complex mediated regulation of epithelial division orientation and cell fate

    Energy Technology Data Exchange (ETDEWEB)

    Vorhagen, Susanne; Niessen, Carien M., E-mail: carien.niessen@uni-koeln.de

    2014-11-01

    Oriented cell division is a key regulator of tissue architecture and crucial for morphogenesis and homeostasis. Balanced regulation of proliferation and differentiation is an essential property of tissues not only to drive morphogenesis but also to maintain and restore homeostasis. In many tissues orientation of cell division is coupled to the regulation of differentiation producing daughters with similar (symmetric cell division, SCD) or differential fate (asymmetric cell division, ACD). This allows the organism to generate cell lineage diversity from a small pool of stem and progenitor cells. Division orientation and/or the ratio of ACD/SCD need to be tightly controlled. Loss of orientation or an altered ratio can promote overgrowth, alter tissue architecture and induce aberrant differentiation, and have been linked to morphogenetic diseases, cancer and aging. A key requirement for oriented division is the presence of a polarity axis, which can be established through cell intrinsic and/or extrinsic signals. Polarity proteins translate such internal and external cues to drive polarization. In this review we will focus on the role of the polarity complex aPKC/Par3/Par6 in the regulation of division orientation and cell fate in different mammalian epithelia. We will compare the conserved function of this complex in mitotic spindle orientation and distribution of cell fate determinants and highlight common and differential mechanisms in which this complex is used by tissues to adapt division orientation and cell fate to the specific properties of the epithelium.

  2. Inflammation and uPAR-Expression in Colorectal Liver Metastases in Relation to Growth Pattern and Neo-adjuvant Therapy

    DEFF Research Database (Denmark)

    Eefsen, R L; Engelholm, L; Alpizar-Alpizar, W;

    2015-01-01

    Proteolytic activity and inflammation in the tumour microenvironment affects cancer progression. In colorectal cancer (CRC) liver metastases it has been observed that three different immune profiles are present, as well as proteolytic activity, determined by the expression of urokinase......-type plasminogen activator (uPAR).The main objectives of this study were to investigate uPAR expression and the density of macrophages (CD68) and T cells (CD3) as markers of inflammation in resected CRC liver metastases, where patients were neo-adjuvantly treated with chemotherapy with or without the angiogenesis...... inhibitor bevacizumab. Chemonaive patients served as a control group. The markers were correlated to growth patterns (GP) of liver metastases, i.e. desmoplastic, pushing and replacement GP. It was hypothesised that differences in proteolysis and inflammation could reflect tumour specific growth and therapy...

  3. Adrenoleucodistrofia ligada ao X: correlação entre o escore de Loes e parâmetros do tensor de difusão

    OpenAIRE

    Sergio Eiji Ono; Arnolfo de Carvalho Neto; Emerson Leandro Gasparetto; Luiz Otávio de Mattos Coelho; Dante Luiz Escuissato; Carmem Maria Sales Bonfim; Lisandro Lima Ribeiro

    2014-01-01

    Objetivo: Avaliar a correlação dos parâmetros do tensor de difusão com o escore de Loes e se os parâmetros do tensor de difusão poderiam indicar alterações estruturais precoces. Materiais e Métodos: As medidas do tensor de difusão foram obtidas em 30 exames de 14 pacientes com adrenoleucodistrofia ligada ao X e foram correlacionadas com o escore de Loes. Um grupo controle de 28 pacientes masculinos foi avaliado para estabelecer medidas do tensor de difusão pareadas por idade. Análises estatís...

  4. Incremento de la competencia lingüística en dos sujetos con parálisis cerebral mediante el dispositivo Makey-Makey

    OpenAIRE

    Calleja Reina, Marina; Luque Liñán, Maria Luisa; Liranza Grajales, Ana Isabel

    2015-01-01

    Incremento de la competencia lingüística en dos sujetos con Parálisis Cerebral mediante el dispositivo Makey-Makey. Un estudio de caso Las personas con PC presentan un riesgo considerable de sufrir problemas de comunicación. Estos problemas de comunicación pueden surgir a consecuencia de déficits en el control motor del habla, en la cognición, en el lenguaje, en la percepción/sensación o una combinación de todos ellos (Hustad & Miles, 2010). En este trabajo se muestran dos casos de Parális...

  5. Efeitos de dieta contendo castanha de caju sobre o desenvolvimento ponderal, parÃmetros seminais e proteoma do plasma seminal de carneiros Morada Nova

    OpenAIRE

    Emerson Pinto Moreira

    2015-01-01

    O presente estudo avaliou os efeitos da inclusÃo de 13% de farelo de castanha de caju (CNM) na dieta de carneiros Morada Nova nos parÃmetros seminais e proteÃnas do plasma seminal. Vinte carneiros foram distribuÃdos em dois grupos iguais: grupo castanha de caju (CNG) e grupo controle (COG). O CNG e COG receberam 13% e 0% de CNM na dieta durante 90 dias. Os grupos foram comparados para peso corporal, circunferÃncia escrotal, parÃmetros seminais e proteÃnas do plasma seminal, utilizando o MÃtod...

  6. NMDA receptor subunit expression and PAR2 receptor activation in colospinal afferent neurons (CANs during inflammation induced visceral hypersensitivity

    Directory of Open Access Journals (Sweden)

    Caudle Robert M

    2009-09-01

    Full Text Available Abstract Background Visceral hypersensitivity is a clinical observation made when diagnosing patients with functional bowel disorders. The cause of visceral hypersensitivity is unknown but is thought to be attributed to inflammation. Previously we demonstrated that a unique set of enteric neurons, colospinal afferent neurons (CANs, co-localize with the NR1 and NR2D subunits of the NMDA receptor as well as with the PAR2 receptor. The aim of this study was to determine if NMDA and PAR2 receptors expressed on CANs contribute to visceral hypersensitivity following inflammation. Recently, work has suggested that dorsal root ganglion (DRG neurons expressing the transient receptor potential vanilloid-1 (TRPV1 receptor mediate inflammation induced visceral hypersensitivity. Therefore, in order to study CAN involvement in visceral hypersensitivity, DRG neurons expressing the TRPV1 receptor were lesioned with resiniferatoxin (RTX prior to inflammation and behavioural testing. Results CANs do not express the TRPV1 receptor; therefore, they survive following RTX injection. RTX treatment resulted in a significant decrease in TRPV1 expressing neurons in the colon and immunohistochemical analysis revealed no change in peptide or receptor expression in CANs following RTX lesioning as compared to control data. Behavioral studies determined that both inflamed non-RTX and RTX animals showed a decrease in balloon pressure threshold as compared to controls. Immunohistochemical analysis demonstrated that the NR1 cassettes, N1 and C1, of the NMDA receptor on CANs were up-regulated following inflammation. Furthermore, inflammation resulted in the activation of the PAR2 receptors expressed on CANs. Conclusion Our data show that inflammation causes an up-regulation of the NMDA receptor and the activation of the PAR2 receptor expressed on CANs. These changes are associated with a decrease in balloon pressure in response to colorectal distension in non-RTX and RTX lesioned

  7. Leprosy New Case Detection Trends and the Future Effect of Preventive Interventions in Pará State, Brazil: A Modelling Study.

    Science.gov (United States)

    de Matos, Haroldo José; Blok, David J; de Vlas, Sake J; Richardus, Jan Hendrik

    2016-03-01

    Leprosy remains a public health problem in Brazil. Although the overall number of new cases is declining, there are still areas with a high disease burden, such as Pará State in the north of the country. We aim to predict future trends in new case detection rate (NCDR) and explore the potential impact of contact tracing and chemoprophylaxis on NCDR in Pará State. We used SIMCOLEP, an existing individual-based model for the transmission and control of M. leprae, in a population structured by households. The model was quantified to simulate the population and observed NCDR of leprosy in Pará State for the period 1990 to 2014. The baseline scenario was the current control program, consisting of multidrug therapy, passive case detection, and active case detection from 2003 onwards. Future projections of the NCDR were made until 2050 given the continuation of the current control program (i.e. baseline). We further investigated the potential impact of two scenarios for future control of leprosy: 1) discontinuation of contact tracing; and 2) continuation of current control in combination with chemoprophylaxis. Both scenarios started in 2015 and were projected until 2050. The modelled NCDR in Pará State after 2014 shows a continuous downward trend, reaching the official elimination target of 10 cases per 100,000 population by 2030. The cessation of systematic contact tracing would not result in a higher NCDR in the long run. Systematic contact tracing in combination with chemoprophylaxis for contacts would reduce the NCDR by 40% and bring attainment of the elimination target two years forward to 2028. The NCDR of leprosy continues to decrease in Pará State. Elimination of leprosy as a public health problem could possibly be achieved around 2030, if the current control program is maintained. Providing chemoprophylaxis would decrease the NCDR further and would bring elimination forward by two years.

  8. Action of vegetal regulators, environmental control and storage over parameters of sweet pepper conservation in postharvest Ação de reguladores vegetais, controle ambiental e armazenamento sobre parâmetros de conservação do pimentão em pós-colheita

    Directory of Open Access Journals (Sweden)

    Elisabeth Orica Ono

    2011-10-01

    Full Text Available O objetivo do trabalho foi de estudar o efeito da aplicação de reguladores vegetais em pós-colheita verificando a conservação do pimentão híbrido Elisa armazenado sob controle ambiental e refrigeração. Os frutos foram resfriados em água por aproximadamente cinco minutos, procedendo-se, em seguida, à secagem natural a sombra. Os tratamentos pós colheita foram constituídos: 1 Testemunha (imersão em água; 2 11,12 ml . 4 L-1 de GA + CK (Ácido giberélico + Citocinina; 3 19,04 ml . 4 L-1 de GA 4 + 7 (Ácido Giberélico; 4 5g . 5 L-1 de GA 3 (Ácido Giberélico; 5 16,68 ml . 3 L-1 de CK (Citocinina. Os tratamentos com hormônios foram diluídos em concentração de 100 mg L-1. Em seguida os frutos foram armazenamento em condições ambientais normais e em câmara fria a uma temperatura de 8 oC e 85-90% UR. O delineamento estatístico adotado foi o de blocos ao acaso com 5 tratamentos e 4 repetições. O experimento foi avaliado do ponto zero até os 28 dias a cada quatro dias, através das seguintes variáveis: cor, firmeza do fruto, sólidos solúveis totais “Brix” (SST, acidez total titulável (ATT, relação SST/ATT e carboidratos solúveis (CHOs. Após 28 dias de armazenamento, os hormônios vegetais ga3, ga4+7 nas concentrações utilizadas foram eficientes em retardar o metabolismo pós-colheita dos frutos de pimentão armazenados em condições ambiente e refrigeradas, bem como em promover barreira contra as perdas de pós-colheita dos frutos de pimentão.

  9. Validation of MODIS and GEOV1 fPAR Products in a Boreal Forest Site in Finland

    Directory of Open Access Journals (Sweden)

    Titta Majasalmi

    2015-01-01

    Full Text Available Remote sensing of the fraction of absorbed Photosynthetically Active Radiation (fPAR has become a timely option to monitor forest productivity. However, only a few studies have had ground reference fPAR datasets containing both forest canopy and understory fPAR from boreal forests for the validation of satellite products. The aim of this paper was to assess the performance of two currently available satellite-based fPAR products: MODIS fPAR (MOD15A2, C5 and GEOV1 fPAR (g2_BIOPAR_FAPAR, as well as an NDVI-fPAR relationship applied to the MODIS surface reflectance product and a Landsat 8 image, in a boreal forest site in Finland. Our study area covered 16 km2 and field data were collected from 307 forest plots. For all plots, we obtained both forest canopy fPAR and understory fPAR. The ground reference total fPAR agreed better with GEOV1 fPAR than with MODIS fPAR, which showed much more temporal variation during the peak-season than GEOV1 fPAR. At the chosen intercomparison date in peak growing season, MODIS NDVI based fPAR estimates were similar to GEOV1 fPAR, and produced on average 0.01 fPAR units smaller fPAR estimates than ground reference total fPAR. MODIS fPAR and Landsat 8 NDVI based fPAR estimates were similar to forest canopy fPAR.

  10. Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles.

    Science.gov (United States)

    Bharat, Tanmay A M; Murshudov, Garib N; Sachse, Carsten; Löwe, Jan

    2015-07-02

    Active segregation of Escherichia coli low-copy-number plasmid R1 involves formation of a bipolar spindle made of left-handed double-helical actin-like ParM filaments. ParR links the filaments with centromeric parC plasmid DNA, while facilitating the addition of subunits to ParM filaments. Growing ParMRC spindles push sister plasmids to the cell poles. Here, using modern electron cryomicroscopy methods, we investigate the structures and arrangements of ParM filaments in vitro and in cells, revealing at near-atomic resolution how subunits and filaments come together to produce the simplest known mitotic machinery. To understand the mechanism of dynamic instability, we determine structures of ParM filaments in different nucleotide states. The structure of filaments bound to the ATP analogue AMPPNP is determined at 4.3 Å resolution and refined. The ParM filament structure shows strong longitudinal interfaces and weaker lateral interactions. Also using electron cryomicroscopy, we reconstruct ParM doublets forming antiparallel spindles. Finally, with whole-cell electron cryotomography, we show that doublets are abundant in bacterial cells containing low-copy-number plasmids with the ParMRC locus, leading to an asynchronous model of R1 plasmid segregation.

  11. Décontamination nucléaire par laser UV

    Science.gov (United States)

    Delaporte, Ph.; Gastaud, M.; Marine, W.; Sentis, M.; Uteza, O.; Thouvenot, P.; Alcaraz, J. L.; Le Samedy, J. M.; Blin, D.

    2003-06-01

    Le développement et l'utilisation de procédés propres pour le nettoyage ou la préparation de surfaces est l'une des priorités du milieu industriel. Cet intérêt est d'autant plus grand dans le domaine du nucléaire pour lequel la réduction des déchets est un axe de recherche important. Un dispositif de décontamination nucléaire par laser UV impulsionnel a été développé et testé. Il est composé. d'un laser à excimères de 1kW, d'un faisceau de fibres optiques et d'un dispositif de récupération des particules. Les essais réalisés en milieu actif ont démontré sa capacité à nettoyer des surfaces métalliques polluées par différents radioéléments avec des facteurs de décontamination généralement supérieurs à 10. Ce dispositif permet de décontaminer de grandes surfaces de géométrie simple en réduisant fortement la génération de déchets secondaires. Il est, à ce jour et dans ces conditions d'utilisations, le procédé de décontamination par voie sèche le plus efficace.

  12. SYNTHESE DES DIENAMINES CYCLIQUES A CONJUGAISON CROISEE PAR THERMOLYSE

    OpenAIRE

    Ghosez, L.; Universidad Católica de Louvain, Laboratorio de Química Orgánica Síntesis Edificio Lavoiser, Lugar Louis Pasteur 1, 1348 Louvain la Neuve-Bélgica; Grimaldo-Moron, J. T.; Universidad Nacional Mayor de San Marcos, Facultad de Química e Ingeniería Química, Departamento de Química Orgánica, Av. Venezuela s/n, Lima-Perú

    2014-01-01

    Triénamines acycliques a conjugaison Croisée donnent lieu a des réactions électrocycliques par thermolyse classique en phase gazeose. Diénamines cycliques a conjugaison croisée sont obtenus avec d'excellents rendements. Trienaminas acíclicas con conjugación cruzada dan lugar a reacciones electrocíclicas por termólisis clásica en fase gaseosa. Dienaminas cíclicas con conjugación cruzada son sintetizadas con excelentes rendimientos.

  13. Reconnaissance coloniale chez des fourmis se reproduisant par bouturage

    OpenAIRE

    Ichinose, K.; Lenoir, Alain; Cerdá, Xim

    2002-01-01

    Certaines fourmis se reproduisant par bouturage, comme les Aphaenogaster senilis, sont peu agressives envers les individus d¿autres colonies proches de la même espèce. Dans ce cas, il est difficile d¿apprécier les relations entre colonies. Nous avons cherché à mettre au point une méthode qui permette de discriminer deux colonies différentes. Nous avons effectué des rencontres entre individus de colonies élevées au laboratoire depuis plus d¿un an, et de nids collectés récemment. Quatre catégor...

  14. Hemangioma capilar de la parótida. Abordaje transoral

    OpenAIRE

    Luis Domingo Sarra; Juan Carlos Rodríguez; Julio Alberto San Martino

    2014-01-01

    Los hemangiomas de la parótida son frecuentes en niños, representando aproximadamente el 50% de los tumores de dicha glándula durante el primer año de vida. Como contrapartida, estos tumores vasculares son extremadamente raros en pacientes adultos y solo se publican unos pocos casos aislados en la literatura, donde se mencionan las dificultades en el diagnóstico diferencial con los tumores primarios de las glándulas salivales. Presentamos un caso en una paciente adulta que consultó por una...

  15. PROTECTION ET DEPROTECTION D’ALCOOLS PAR REDUCTION ELECTROCHIMIQUE

    OpenAIRE

    A FIALA; A CHIBANI; F LOUAFI

    2007-01-01

    Le groupement ortho-nitrobenzoyle a été utilisé comme groupe protecteur dans le cas d'amino-acétophénones et de plusieurs amino-acides ou esters. Dans le cas des alcools, la déprotection d’esters correspondants, par voie électrochimique, nécessite un faible potentiel cathodique pour l’obtention d'hydroxylamine ; en milieu acide, cette dernière se cyclise quantitativement pour donner la benzisoxazolone avec libération de l'alcool de départ.

  16. Puesta en marcha del sensor fuerza-par JR3

    OpenAIRE

    Hoz Najarro, Carlos de la

    2011-01-01

    Este estudio se engloba dentro de los proyectos realizados por el grupo de trabajo Robotic Lab de la Universidad Carlos III de Madrid para el desarrollo del nuevo Humanoide RH-2. Concretamente, implementaremos sobre software libre (Linux) una aplicación C++ que nos permita obtener de la tarjeta de adquisición de datos de 4 puertos PCI P/N 1593 de JR3 inc., en tiempo real, los valores de fuerza/par del sensor industrial Force Moment Sensor 85M35A3-I40-DH12, de la compañía JR3 inc., que llevará...

  17. Caso práctico parálisis cerebral

    OpenAIRE

    Marcos Gómez, Sergio; Queimadelos Navarro, Sofía; Rodríguez Martín, Samuel; Sañudo Herrero, Alba

    2016-01-01

    El presente trabajo está enfocado al área psicomotor, siguiendo las bases de la intervención de Atención Temprana, desde el campo de la Terapia Ocupacional en la afectación de Parálisis Cerebral Infantil (PCI). El tratamiento empleado está dirigido a potenciar la funcionalidad del miembro afecto, para facilitar al paciente su desempeño en las Actividades de la Vida Diaria, Ocio y Tiempo Libre. Tras llevar a cabo la intervención, se realizó una recogida de datos y se analizó si los objet...

  18. Etiología en parálisis cerebral.

    OpenAIRE

    Campos,Patricia; Bancalari, Ernesto; Castañeda, Carlos

    2013-01-01

    Objetivo: Determinar las causas mas frecuentes de parálisis cerebral (PC). Material y métodos: Estudio descriptivo realizado en Hospital Nacional Cayetano Heredia y el Hogar Clínica San Juan de Dios (Lima-Perú), entre noviembre 1990 y mayo de 1992. Se revisaron las historias clínicas de 102 pacientes con diagnósticos de PC y se revisó las etiologías. Resultados: En relación a edad gestacional hubieron 15 casos de prematuros (14.7%), 67(65%) a término y un postmaduro, en 19 no se tuvo la edad ...

  19. Photogrammétrie et vision par ordinateur

    OpenAIRE

    Kalantari, Mahzad; Kasser, Michel

    2008-01-01

    National audience; Une comparaison est effectuée entre les façons employées en photogrammétrie et en vision par ordinateur pour traiter les problèmes liés à l'acquisition de la 3D à partir d'images stéréoscopiques. Le formalisme adopté, assez différent, est présenté, et quelques perspectives d'évolution en sont déduites.

  20. La couleur en vision par ordinateur : 1.une revue

    OpenAIRE

    Luong, Quang-Tuan

    1990-01-01

    L'utilisation de la couleur en vision par ordinateur est un sujet de recherche qui suscite un interet recent et croissant. Ce papier fait le point dans ce domaine, en essayant de repondre aux questions : Qu'est-ce que la couleur ? Quelles en sont les representations adequates ? Comment la determiner ? Que peut-on en faire ? Pour cela, nous faisons une revue approfondie et tres a jour de l'ensemble de la litterature consacree a ce sujet en cernant les axes de recherche et les problematiques im...

  1. Cartographie par carroyage et précision spatiale

    Directory of Open Access Journals (Sweden)

    Patrice LANGLOIS

    1998-03-01

    Full Text Available Dans le cadre d'une cartographie par carroyage, on étudie la désagrégation de variables zonales, en revenant sur l'hypothèse de l'équirépartition spatiale des populations. L'objectif est d'approcher des règles différenciées de désagrégation des variables en fusionnant des plans d'information de nature morphologique, topologique et environnementale.

  2. Frittage ultra-rapide naturel : chauffage par micro-ondes et par induction

    OpenAIRE

    Guyon, Audrey

    2013-01-01

    The techniques of ultrafast pressureless sintering as microwave or induction sintering offer manyadvantages. However, the development of these techniques requires an understanding and a control ofthe mechanisms involved. Both similar and complementary, these processes of heating-sintering havebeen studied by an experimental approach to increase knowledge in the field of Ultrafast PressurelessSintering.In this thesis, the study of microwave sintering of Al2O3-(Y)ZrO2 composites has been conduc...

  3. Number and brightness image analysis reveals ATF-induced dimerization kinetics of uPAR in the cell membrane.

    Science.gov (United States)

    Hellriegel, Christian; Caiolfa, Valeria R; Corti, Valeria; Sidenius, Nicolai; Zamai, Moreno

    2011-09-01

    We studied the molecular forms of the GPI-anchored urokinase plasminogen activator receptor (uPAR-mEGFP) in the human embryo kidney (HEK293) cell membrane and demonstrated that the binding of the amino-terminal fragment (ATF) of urokinase plasminogen activator is sufficient to induce the dimerization of the receptor. We followed the association kinetics and determined precisely the dimeric stoichiometry of uPAR-mEGFP complexes by applying number and brightness (N&B) image analysis. N&B is a novel fluctuation-based approach for measuring the molecular brightness of fluorophores in an image time sequence in live cells. Because N&B is very sensitive to long-term temporal fluctuations and photobleaching, we have introduced a filtering protocol that corrects for these important sources of error. Critical experimental parameters in N&B analysis are illustrated and analyzed by simulation studies. Control experiments are based on mEGFP-GPI, mEGFP-mEGFP-GPI, and mCherry-GPI, expressed in HEK293. This work provides a first direct demonstration of the dimerization of uPAR in live cells. We also provide the first methodological guide on N&B to discern minor changes in molecular composition such as those due to dimerization events, which are involved in fundamental cell signaling mechanisms.

  4. The Par3 polarity protein is an exocyst receptor essential for mammary cell survival

    Science.gov (United States)

    Ahmed, Syed Mukhtar; Macara, Ian G.

    2017-01-01

    The exocyst is an essential component of the secretory pathway required for delivery of basolateral proteins to the plasma membranes of epithelial cells. Delivery occurs adjacent to tight junctions (TJ), suggesting that it recognizes a receptor at this location. However, no such receptor has been identified. The Par3 polarity protein associates with TJs but has no known function in membrane traffic. We now show that, unexpectedly, Par3 is essential for mammary cell survival. Par3 silencing causes apoptosis, triggered by phosphoinositide trisphosphate depletion and decreased Akt phosphorylation, resulting from failure of the exocyst to deliver basolateral proteins to the cortex. A small region of PAR3 binds directly to Exo70 and is sufficient for exocyst docking, membrane-protein delivery and cell survival. PAR3 lacking this domain can associate with the cortex but cannot support exocyst function. We conclude that Par3 is the long-sought exocyst receptor required for targeted membrane-protein delivery. PMID:28358000

  5. Paracrine Apoptotic Effect of p53 Mediated by Tumor Suppressor Par-4

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    Ravshan Burikhanov

    2014-01-01

    Full Text Available The guardian of the genome, p53, is often mutated in cancer and may contribute to therapeutic resistance. Given that p53 is intact and functional in normal tissues, we harnessed its potential to inhibit the growth of p53-deficient cancer cells. Specific activation of p53 in normal fibroblasts selectively induced apoptosis in p53-deficient cancer cells. This paracrine effect was mediated by p53-dependent secretion of the tumor suppressor Par-4. Accordingly, the activation of p53 in normal mice, but not p53−/− or Par-4−/− mice, caused systemic elevation of Par-4, which induced apoptosis of p53-deficient tumor cells. Mechanistically, p53 induced Par-4 secretion by suppressing the expression of its binding partner, UACA, which sequesters Par-4. Thus, normal cells can be empowered by p53 activation to induce Par-4 secretion for the inhibition of therapy-resistant tumors.

  6. Chloroquine-Inducible Par-4 Secretion Is Essential for Tumor Cell Apoptosis and Inhibition of Metastasis

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    Ravshan Burikhanov

    2017-01-01

    Full Text Available The induction of tumor suppressor proteins capable of cancer cell apoptosis represents an attractive option for the re-purposing of existing drugs. We report that the anti-malarial drug, chloroquine (CQ, is a robust inducer of Par-4 secretion from normal cells in mice and cancer patients in a clinical trial. CQ-inducible Par-4 secretion triggers paracrine apoptosis of cancer cells and also inhibits metastatic tumor growth. CQ induces Par-4 secretion via the classical secretory pathway that requires the activation of p53. Mechanistically, p53 directly induces Rab8b, a GTPase essential for vesicle transport of Par-4 to the plasma membrane prior to secretion. Our findings indicate that CQ induces p53- and Rab8b-dependent Par-4 secretion from normal cells for Par-4-dependent inhibition of metastatic tumor growth.

  7. På sviktende kunnskapsgrunnlag? Assistert befruktning for lesbiske par

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    Sigrun Saur Stiklestad

    2009-11-01

    Full Text Available Artikkelen drøfter i hvilken grad den norske loven om assistert befruktning for lesbiske par vektlegger forskningsbasert kunnskap om barnas oppvekstvilkår. En kunnskapsteoretisk diskusjon om forholdet mellom forskning og politikk er sentral i denne drøftingen. Artikkelens empiriske grunnlag er i hovedsak dokumenter om lovprosessen, resultater fra forskningsfeltet generelt og tilsva­rende lovprosess i Sverige. Dessuten drøftes kritikken rettet mot forskningsfel­tet. Artikkelen viser at forskningsbasert kunnskap er gitt så lav prioritet i det norske lovarbeidet at det har skapt tvil om kunnskapsstatus på området. Sam­tidig synliggjør artikkelen at det foreligger mer forskning på aktuelle forsk­ningsfelt, som samlet sett går i favør av assistert befruktning for lesbiske par.http://dx.doi.org/10.5324/eip.v3i2.1720

  8. Four cases of endophthalmitis after 25-gauge pars plana vitrectomy

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    Mutoh T

    2012-08-01

    Full Text Available Tetsuya Mutoh, Koji Kadoya, Makoto ChikudaDepartment of Ophthalmology, Dokkyo Medical University Koshigaya Hospital, Koshigaya, Saitama, JapanAbstract: We report our recent experience with four cases of endophthalmitis (one male, three females after 25-gauge pars plana vitrectomy (PPV. One was a case of persistent cystoid macular edema caused by branch retinal vein occlusion, whereas the remaining three were cases of epiretinal membrane. Preoperative antibiotics before the first PPV procedure were not administered in three of the four cases. Endophthalmitis occurred 2–4 days after the first procedure in all cases, for which ceftazidime 2.0 mg/0.1 mL and vancomycin 1.0 mg/0.1 mL were injected into the vitreous cavity. This was followed by emergent 20-gauge PPV and intraocular lens removal using an infusion fluid containing ceftazidime and vancomycin. After the second PPV procedure, progress was good in three cases while retinal detachment occurred in the remaining case one month after surgery; this case required a third PPV procedure. Final best-corrected visual acuity ranged from 20/100 to 20/25 for the four cases. Bacterial cultures were negative after the second PPV procedure in all cases. In conclusion, postoperative endophthalmitis occurred in four of 502 cases (0.80% that underwent 25-gauge PPV at our hospital. It is important to minimize the incidence of endophthalmitis after 25-gauge PPV.Keywords: 25-gauge pars plana vitrectomy, endophthalmitis, incidence

  9. Spectral analysis by correlation; Analyse spectrale par correlation

    Energy Technology Data Exchange (ETDEWEB)

    Fauque, J.M.; Berthier, D.; Max, J.; Bonnet, G. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1969-07-01

    The spectral density of a signal, which represents its power distribution along the frequency axis, is a function which is of great importance, finding many uses in all fields concerned with the processing of the signal (process identification, vibrational analysis, etc...). Amongst all the possible methods for calculating this function, the correlation method (correlation function calculation + Fourier transformation) is the most promising, mainly because of its simplicity and of the results it yields. The study carried out here will lead to the construction of an apparatus which, coupled with a correlator, will constitute a set of equipment for spectral analysis in real time covering the frequency range 0 to 5 MHz. (author) [French] La densite spectrale d'un signal qui represente la repartition de sa puissance sur l'axe des frequences est une fonction de premiere importance, constamment utilisee dans tout ce qui touche le traitement du signal (identification de processus, analyse de vibrations, etc...). Parmi toutes les methodes possibles de calcul de cette fonction, la methode par correlation (calcul de la fonction de correlation + transformation de Fourier) est tres seduisante par sa simplicite et ses performances. L'etude qui est faite ici va deboucher sur la realisation d'un appareil qui, couple a un correlateur, constituera un ensemble d'analyse spectrale en temps reel couvrant la gamme de frequence 0 a 5 MHz. (auteur)

  10. Traumatic ghost cell glaucoma with successful resolution of corneal blood staining following pars Plana vitrectomy

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    Amal Alamri

    2016-01-01

    Full Text Available Ghost cell glaucoma (GCG was first described in 1976. It is a type of a secondary open angle glaucoma, which occurs following long-standing vitreous hemorrhage. The ghost cells are rigid and less pliable than fresh red blood cells; therefore, they may cause direct obstruction of the trabecular meshwork and secondary increase in the intraocular pressure (IOP. This case report presents the diagnosis and management of a rare case of traumatic GCG after vitreous hemorrhage in a phakic child. Pars plana vitrectomy was done after unsuccessful medical therapy and the diagnosis was confirmed by cytopathology. Surprisingly, spontaneous resolution of the corneal blood staining occurred. The outcome in this case was favorable with controlled IOP in the affected eye.

  11. Alpine grassland fPAR change over the Northern Tibetan Plateau from 2002 to 2011

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    Bao-Xiong Chen

    2017-06-01

    Full Text Available In this study, two different methods including Digital Camera and Reference Panel (DCRP and traditional in situ fPAR observation for measuring the in situ point fPAR of very short alpine grass vegetation were compared, and the Moderate Resolution Imaging Spectroradiometer (MODIS fPAR products were evaluated and validated by in situ point data on the alpine grassland over the Northern Tibetan Plateau, which is sensitive to climate change and vulnerable to anthropogenic activities. Results showed that the MODIS alpine grassland fPAR product, examined by using DCRP, and traditional in situ fPAR observation had a significant relationship at the spatial and temporal scales. The decadal MODIS fPAR trend analysis showed that, average growing season fPAR increased by 1.2 × 10−4 per year and in total increased 0.86% from 2002 to 2011 in alpine grassland, when most of the fPAR increments occurred in southeast and center of the Northern Tibetan Plateau, the alpine grassland tended to recover from degradation slightly. However, climatic factors have influenced the various alpine grassland vegetation fPAR over a period of 10 years; precipitation significantly affected the alpine meadow fPAR in the eastern region, whereas temperature considerably influenced the alpine desert steppe fPAR in the west region. These findings suggest that the regional heterogeneity in alpine grassland fPAR results from various environmental factors, except for vegetation characteristics, such as canopy structure and leaf area.

  12. The two Cis-acting sites, parS1 and oriC1, contribute to the longitudinal organisation of Vibrio cholerae chromosome I.

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    Ariane David

    2014-07-01

    Full Text Available The segregation of bacterial chromosomes follows a precise choreography of spatial organisation. It is initiated by the bipolar migration of the sister copies of the replication origin (ori. Most bacterial chromosomes contain a partition system (Par with parS sites in close proximity to ori that contribute to the active mobilisation of the ori region towards the old pole. This is thought to result in a longitudinal chromosomal arrangement within the cell. In this study, we followed the duplication frequency and the cellular position of 19 Vibrio cholerae genome loci as a function of cell length. The genome of V. cholerae is divided between two chromosomes, chromosome I and II, which both contain a Par system. The ori region of chromosome I (oriI is tethered to the old pole, whereas the ori region of chromosome II is found at midcell. Nevertheless, we found that both chromosomes adopted a longitudinal organisation. Chromosome I extended over the entire cell while chromosome II extended over the younger cell half. We further demonstrate that displacing parS sites away from the oriI region rotates the bulk of chromosome I. The only exception was the region where replication terminates, which still localised to the septum. However, the longitudinal arrangement of chromosome I persisted in Par mutants and, as was reported earlier, the ori region still localised towards the old pole. Finally, we show that the Par-independent longitudinal organisation and oriI polarity were perturbed by the introduction of a second origin. Taken together, these results suggest that the Par system is the major contributor to the longitudinal organisation of chromosome I but that the replication program also influences the arrangement of bacterial chromosomes.

  13. The two Cis-acting sites, parS1 and oriC1, contribute to the longitudinal organisation of Vibrio cholerae chromosome I.

    Science.gov (United States)

    David, Ariane; Demarre, Gaëlle; Muresan, Leila; Paly, Evelyne; Barre, François-Xavier; Possoz, Christophe

    2014-07-01

    The segregation of bacterial chromosomes follows a precise choreography of spatial organisation. It is initiated by the bipolar migration of the sister copies of the replication origin (ori). Most bacterial chromosomes contain a partition system (Par) with parS sites in close proximity to ori that contribute to the active mobilisation of the ori region towards the old pole. This is thought to result in a longitudinal chromosomal arrangement within the cell. In this study, we followed the duplication frequency and the cellular position of 19 Vibrio cholerae genome loci as a function of cell length. The genome of V. cholerae is divided between two chromosomes, chromosome I and II, which both contain a Par system. The ori region of chromosome I (oriI) is tethered to the old pole, whereas the ori region of chromosome II is found at midcell. Nevertheless, we found that both chromosomes adopted a longitudinal organisation. Chromosome I extended over the entire cell while chromosome II extended over the younger cell half. We further demonstrate that displacing parS sites away from the oriI region rotates the bulk of chromosome I. The only exception was the region where replication terminates, which still localised to the septum. However, the longitudinal arrangement of chromosome I persisted in Par mutants and, as was reported earlier, the ori region still localised towards the old pole. Finally, we show that the Par-independent longitudinal organisation and oriI polarity were perturbed by the introduction of a second origin. Taken together, these results suggest that the Par system is the major contributor to the longitudinal organisation of chromosome I but that the replication program also influences the arrangement of bacterial chromosomes.

  14. Rôle de la dispersion dans la réponse des populations de truites aux habitats formés par les grands débris ligneux dans les ruisseaux de montagne du Colorado

    Directory of Open Access Journals (Sweden)

    FAUSCH K. D.

    1995-04-01

    Full Text Available Un peu partout dans le monde, les aménagistes des pêches utilisent souvent des rondins pour réaliser des mouilles pour les salmonidés, souvent pour compenser l'absence de grands débris ligneux (LWD due à la déforestation ou à d'autres perturbations des forêts alluviales. Des mesures des LWD réalisées sur 11 ruisseaux de montagne du Colorado drainant de vieilles forêts de résineux (Picea - Abies montrent qu'ils sont à l'origine de la plupart des mouilles. Les morceaux qui contribuent à la formation des mouilles sont, en moyenne, plus grands que ceux qui n'en forment pas. La plupart enjambe le chenal perpendiculairement à l'écoulement et forme des mouilles de chute («plunge pool» et des mouilles de retenue («dammed pool». Les aménagistes des pêches qui utilisent des troncs perpendiculaires à l'écoulement pour réaliser des mouilles considèrent généralement que cette addition d'habitat améliore la survie des salmonidés résidents pendant les périodes critiques comme l'hiver. Une expérience de longue durée, réalisée pour tester cette hypothèse dans six ruisseaux de montagne du Colorado, a montré que les populations de truites résidentes augmentaient rapidement et significativement dans des portions expérimentales de 250m, par rapport à des témoins adjacents. Cependant, des mesures de la dispersion réalisées par des piégeages et des recaptures de truites marquées ont montré que les mouilles formées par des troncs augmentent les populations de truites adultes en permettant aux truites qui étaient en phase de déplacement de rester dans les portions expérimentales plutôt qu'en améliorant la survie hivernale sur place, comme le décrivent d'autres auteurs. La recherche sur les salmonidés d'eau courante non migrateurs et leur gestion a jusqu'à présent été marquée par un modèle impliquant des déplacements limités, ce qui implique que les adultes soient relativement sédentaires. Cependant, l

  15. Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology

    DEFF Research Database (Denmark)

    Bache, Michael; Bosco, Filippo; Brøgger, Anna Line

    2013-01-01

    In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels...... of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody–antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable...

  16. SuPAR Predicts Cardiovascular Events and Mortality in Patients With Asymptomatic Aortic Stenosis

    DEFF Research Database (Denmark)

    Hodges, Gethin W; Bang, Casper N; Eugen-Olsen, Jesper

    2016-01-01

    BACKGROUND: Soluble urokinase plasminogen activator receptor (suPAR) is an inflammatory marker associated with subclinical cardiovascular damage and cardiovascular events. Whether suPAR is of prognostic value in asymptomatic patients with aortic stenosis (AS) remains unknown. METHODS: Plasma su......PAR levels were measured in 1503 patients with a mean age of 68 years who were recruited in the Simvastatin and Ezetimibe in Aortic Stenosis (SEAS) study. Cox regression analysis was performed to evaluate associations between suPAR and the composite end points of ischemic cardiovascular events (ICEs), aortic...

  17. The polarity protein Par3 regulates APP trafficking and processing through the endocytic adaptor protein Numb.

    Science.gov (United States)

    Sun, Miao; Asghar, Suwaiba Z; Zhang, Huaye

    2016-09-01

    The processing of amyloid precursor protein (APP) into β-amyloid peptide (Aβ) is a key step in the pathogenesis of Alzheimer's disease (AD), and trafficking dysregulations of APP and its secretases contribute significantly to altered APP processing. Here we show that the cell polarity protein Par3 plays an important role in APP processing and trafficking. We found that the expression of full length Par3 is significantly decreased in AD patients. Overexpression of Par3 promotes non-amyloidogenic APP processing, while depletion of Par3 induces intracellular accumulation of Aβ. We further show that Par3 functions by regulating APP trafficking. Loss of Par3 decreases surface expression of APP by targeting APP to the late endosome/lysosome pathway. Finally, we show that the effects of Par3 are mediated through the endocytic adaptor protein Numb, and Par3 functions by interfering with the interaction between Numb and APP. Together, our studies show a novel role for Par3 in regulating APP processing and trafficking.

  18. Tryptase-PAR2 axis in experimental autoimmune prostatitis, a model for chronic pelvic pain syndrome.

    Science.gov (United States)

    Roman, Kenny; Done, Joseph D; Schaeffer, Anthony J; Murphy, Stephen F; Thumbikat, Praveen

    2014-07-01

    Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) affects up to 15% of the male population and is characterized by pelvic pain. Mast cells are implicated in the murine experimental autoimmune prostatitis (EAP) model as key to chronic pelvic pain development. The mast cell mediator tryptase-β and its cognate receptor protease-activated receptor 2 (PAR2) are involved in mediating pain in other visceral disease models. Prostatic secretions and urines from CP/CPPS patients were examined for the presence of mast cell degranulation products. Tryptase-β and PAR2 expression were examined in murine EAP. Pelvic pain and inflammation were assessed in the presence or absence of PAR2 expression and upon PAR2 neutralization. Tryptase-β and carboxypeptidase A3 were elevated in CP/CPPS compared to healthy volunteers. Tryptase-β was capable of inducing pelvic pain and was increased in EAP along with its receptor PAR2. PAR2 was required for the development of chronic pelvic pain in EAP. PAR2 signaling in dorsal root ganglia led to extracellular signal-regulated kinase (ERK)1/2 phosphorylation and calcium influx. PAR2 neutralization using antibodies attenuated chronic pelvic pain in EAP. The tryptase-PAR2 axis is an important mediator of pelvic pain in EAP and may play a role in the pathogenesis of CP/CPPS.

  19. TRIM21 is a novel regulator of Par-4 in colon and pancreatic cancer cells

    Science.gov (United States)

    Nguyen, Jeffrey Q.; Irby, Rosalyn B.

    2017-01-01

    ABSTRACT The prostate apoptosis response protein 4 (Par-4) is a tumor-suppressor that has been shown to induce cancer-cell selective apoptosis in a variety of cancers. The regulation of Par-4 expression and activity is a relatively understudied area, and identifying novel regulators of Par-4 may serve as novel therapeutic targets. To identify novel regulators of Par-4, a co-immunoprecipitation was performed in colon cancer cells, and co-precipitated proteins were identified by mass-spectometry. TRIM21 was identified as a novel interacting partner of Par-4, and further shown to interact with Par-4 endogenously and through its PRY-SPRY domain. Additional studies show that TRIM21 downregulates Par-4 levels in response to cisplatin, and that TRIM21 can increase the resistance of colon cancer cells to cisplatin. Furthermore, forced Par-4 expression can sensitize pancreatic cancer cells to cisplatin. Finally, we demonstrate that TRIM21 expression predicts survival in pancreatic cancer patients. Our work highlights a novel mechanism of Par-4 regulation, and identifies a novel prognostic marker and potential therapeutic target for pancreatic cancer. PMID:27830973

  20. Comparative validation of the IPAQ and the 7-Day PAR among women diagnosed with breast cancer

    Directory of Open Access Journals (Sweden)

    Rock Cheryl L

    2006-03-01

    Full Text Available Abstract Background The criterion-related validity and measurement bias of the long form of the International Physical Activity Questionnaire (IPAQ was compared to the 7-Day Physical Activity Recall (PAR. Methods Participants were women who have been diagnosed with breast cancer and enrolled in the ongoing Women's Healthy Eating and Living Study. Women (N = 159, average age 57 years wore an accelerometer for one week and then completed the IPAQ or the PAR. Results The validity correlation of the PAR was significantly higher (p Conclusion The PAR was superior to the IPAQ in terms of validity, measurement bias, and screening statistics.

  1. Tryptase - PAR2 axis in Experimental Autoimmune Prostatitis, a model for Chronic Pelvic Pain Syndrome

    Science.gov (United States)

    Roman, Kenny; Done, Joseph D.; Schaeffer, Anthony J.; Murphy, Stephen F.; Thumbikat, Praveen

    2014-01-01

    Chronic prostatitis/Chronic pelvic pain syndrome (CP/CPPS) affects up to 15% of the male population and is characterized by pelvic pain. Mast cells are implicated in the murine experimental autoimmune prostatitis (EAP) model as key to chronic pelvic pain development. The mast cell mediator tryptase-β and its cognate receptor protease-activated receptor 2 (PAR2) are involved in mediating pain in other visceral disease models. Prostatic secretions and urines from CP/CPPS patients were examined for the presence of mast cell degranulation products. Tryptase-β and PAR2 expression were examined in murine experimental autoimmune prostatitis (EAP). Pelvic pain and inflammation were assessed in the presence or absence of PAR2 expression and upon PAR2 neutralization. Tryptase-β and carboxypeptidase A3 were elevated in CP/CPPS compared to healthy volunteers. Tryptase-β was capable of inducing pelvic pain and was increased in EAP along with its receptor PAR2. PAR2 was required for the development of chronic pelvic pain in EAP. PAR2 signaling in dorsal root ganglia lead to ERK1/2 phosphorylation and calcium influx. PAR2 neutralization using antibodies attenuated chronic pelvic pain in EAP. The tryptase-PAR2 axis is an important mediator of pelvic pain in EAP and may play a role in the pathogenesis of CP/CPPS. PMID:24726923

  2. A Desired PAR-Achieving Precoder Design for Multiuser MIMO OFDM Based on Concentration of Measure

    Science.gov (United States)

    Cha, Hyun-Su; Kim, Dong Ku

    2017-03-01

    For multi-user multiple-input and multiple-output (MIMO) wireless communications in orthogonal frequency di- vision multiplexing systems, we propose a MIMO precoding scheme providing a desired peak-to-average power ratio (PAR) at the minimum cost that is defined as received SNR degradation. By taking advantage of the concentration of measure, we formulate a convex problem with constraint on the desired PAR. Consequently, the proposed scheme almost exactly achieves the desired PAR on average, and asymptotically attains the desired PAR at the 0.001 point of its complementary cumulative distribution function, as the number of subcarriers increases.

  3. Proxemic-aware controls

    DEFF Research Database (Denmark)

    Ledo, David; Greenberg, Saul; Marquardt, Nicolai

    2015-01-01

    Remote controls facilitate interactions at-a-distance with appliances. However, the complexity, diversity, and in-creasing number of digital appliances in ubiquitous com-puting ecologies make it increasingly difficult to: (1) dis-cover which appliances are controllable; (2) select a par-ticular a...

  4. IGF-II receptors in luminal and basolateral membranes isolated from pars convoluta and pars recta of rabbit proximal tubule

    DEFF Research Database (Denmark)

    Jacobsen, Christian; Jessen, H; Flyvbjerg, A

    1995-01-01

    The binding of 125I-labeled insulin-like growth factor-II (125I-IGF-II) to luminal and basolateral membrane vesicles isolated from pars convoluta and the straight part (pars recta) of rabbit proximal tubule was investigated. Analyses of the binding data by use of the general stoichiometric binding...... equation revealed, that in all preparations IGF-II was bound to one high-affinity binding site and other sites with lower affinities. The specificity of the high-affinity 125I-IGF-II binding to the membrane vesicles assessed by displacement by unlabeled IGF-II, IGF-I and insulin showed that IGF-I displaced...... 125I-IGF-II in the range 22.5-47.9 nM (IC50) whereas insulin did not effect 125I-IGF-II binding at all. beta-Galactosidase inhibited the 125I-IGF-II binding with half-maximal inhibition of 20-30 nM beta-galactosidase. D-Mannose 6-phosphate increased the binding of 125I-IGF-II and reversed...

  5. Un « gouvernement des journalistes par le Pouvoir politique » par défaut ?

    Directory of Open Access Journals (Sweden)

    Matthieu Lardeau

    2013-12-01

    Full Text Available Cet article présente les interactions de deux mouvements concomitants apparusprincipalement dans les décennies 1950 à 1970 dans le champ de la presse française : d’un côté les initiatives – consécutives à la démarche pionnière, en 1951, de création de la première Société des journalistes (SDJ au sein du Monde – menées par les journalistes de rédactions de quotidiens pour créer des SDJ dont la destination peut aller jusqu’à gouverner les journaux; de l’autre côté, les « réponses » apportées par les Pouvoirs politique et public pour contenir ces initiatives visant à étendre le pouvoir des journalistes dans la gestion et la gouvernance de leurs journaux. Cette étude exploratoire repose principalement sur l’analyse de deux types de littérature, souvent déconsidérés ou négligés par le champ académique, en dépit de leur grande richesse : (1 la littérature professionnelle, ayant pour principaux auteurs les journalistes et managers de presse (Périer Daville, Boegner, Pigasse, etc., florissante depuis 1944 et singulièrement durant les décennies 1960 et 1970 ; (2 la littérature grise constituée notamment par des rapports publics rédigés par des institutions comme les services du Premier ministre (commissions Lindon de 1970 et Serisé de 1972, le Conseil économique et social, etc. Nos principaux résultats montrent que les propositions ou actions menées par les journalistes (1 restent le plus souvent inexploitées in concreto par les journalistes eux-mêmes; (2 entrent le plus souvent en opposition avec les politiques de nouveaux actionnaires ou directions de journaux et avec les projets des autorités politiques et publiques qui cherchent à encadrer l’autonomie de la profession journalistique pour mieux gouverner celle-ci ; (3 combien in fine les représentants des différents acteurs impliqués s’accordent pour contenir l’émergence d’un « gouvernement des journalistes » par les

  6. «Interpréter Aristote par Aristote.»

    Directory of Open Access Journals (Sweden)

    Luca Bianchi

    2004-04-01

    Full Text Available On peut remettre en question plusieurs schémas conceptuels utilisés par les historiens de l’herméneutique si l’on tient compte de l’histoire de traditions philosophiques qui ne devraient pas être négligées par ceux qui s’attachent à reconstruire le développement des notions et des méthodes herméneutiques. Centré sur la tradition aristotélicienne, cet article a pour but de montrer : 1 qu’entre le Moyen Âge et la Renaissance, le sens du terme latin interpretatio a sensiblement changé ; 2 que l’approche humaniste du corpus aristotélicien se base sur le principe que tout auteur est le meilleur interprète de lui-même ; 3 que ce principe était formulé et discuté dans certains traités, négligés, qui portaient sur l’optima ratio interpretandi Aristotelem et furent publiés par Nuñez en 1554, Pascual en 1591 et Piccart en 1605.Several conceptual schemes generally used by historians of hermeneutics can be challenged if one takes into consideration the history of philosophical traditions, which should not be neglected by those interested in reconstructing the development of hermeneutical notions and methods. Focusing on the Aristotelian tradition, this article aims at showing : 1 that between the Middle Ages and the Renaissance the meaning of the Latin term interpretatio shifted significantly ; 2 that the humanists’ approach to the Aristotelian corpus is based on the principle that every author is the best interpreter of himself ; 3 that this principle was formulated and discussed in some neglected treatises dealing with the optima ratio interpretandi Aristotelem, published by Nuñez in 1554, by Pascual in 1591, and by Piccart in 1605.

  7. Analyse du comportement thermique du sol d'une serre agricole par simulation dynamique

    Science.gov (United States)

    Monteil, C.; Amouroux, M.

    1997-02-01

    The overall energetical behaviour of a greenhouse is significantly affected by diurnal storage and nocturnal release of energy inside the soil, which is the only element with high thermal capacitance. Its specific part is analysed with a pre-existing dynamic simulation model. The first part of this paper studies the effects of 4kinds of heating exchangers along each month of an entire cultivation period: hot air convector, aerial metallic pipes with hot water circulation, radiant mulch or buried pipes with warm water circulation. The second part develops the hourly behaviour of both greenhouse and soil during a cold standard-day with each of the 4exchangers. The third part is a sensibility study to various classes of factors in the case of hot air convector exchanger: climatic environment, control policy, intrinsic properties of the greenhouse (soil characteristics, crop density, cover transparency). Le comportement énergétique global d'une serre agricole est influencé de manière parfois importante par le stockage diurne et la restitution nocturne d'énergie au sein de son sol, seul élément à grande capacité thermique. Le rôle spécifique joué par le sol est analysé en appliquant un modèle de simulation dynamique déjà existant. Une première partie compare les effets de 4types d'échangeurs de chauffage sur chaque mois d'une période de culture : aérotherme, tubes aériens à circulation d'eau chaude, paillage radiant ou tubes enterrés à circulation d'eau tiède. Une seconde partie développe le comportement horaire de la serre et du sol pour une journée-type froide en présence de chacun des échangeurs. Une troisième partie précise la sensibilité du bilan thermique de la serre et du sol à différents types de facteurs dans le cas d'un échangeur par aérotherme: environnement climatique, politique de contrôle de la serre, propriétés intrinsèques de la serre (caractéristiques du sol, densité de culture, transparence de la couverture).

  8. Common variants in the human platelet PAR4 thrombin receptor alter platelet function and differ by race

    Science.gov (United States)

    Edelstein, Leonard C.; Simon, Lukas M.; Lindsay, Cory R.; Kong, Xianguo; Teruel-Montoya, Raúl; Tourdot, Benjamin E.; Chen, Edward S.; Ma, Lin; Coughlin, Shaun; Nieman, Marvin; Holinstat, Michael; Shaw, Chad A.

    2014-01-01

    Human platelets express 2 thrombin receptors: protease-activated receptor (PAR)-1 and PAR4. Recently, we reported 3.7-fold increased PAR4-mediated aggregation kinetics in platelets from black subjects compared with white subjects. We now show that platelets from blacks (n = 70) express 14% more PAR4 protein than those from whites (n = 84), but this difference is not associated with platelet PAR4 function. Quantitative trait locus analysis identified 3 common single nucleotide polymorphisms in the PAR4 gene (F2RL3) associated with PAR4-induced platelet aggregation. Among these single nucleotide polymorphisms, rs773902 determines whether residue 120 in transmembrane domain 2 is an alanine (Ala) or threonine (Thr). Compared with the Ala120 variant, Thr120 was more common in black subjects than in white subjects (63% vs 19%), was associated with higher PAR4-induced human platelet aggregation and Ca2+ flux, and generated greater inositol 1,4,5-triphosphate in transfected cells. A second, less frequent F2RL3 variant, Phe296Val, was only observed in blacks and abolished the enhanced PAR4-induced platelet aggregation and 1,4,5-triphosphate generation associated with PAR4-Thr120. PAR4 genotype did not affect vorapaxar inhibition of platelet PAR1 function, but a strong pharmacogenetic effect was observed with the PAR4-specific antagonist YD-3 [1-benzyl-3(ethoxycarbonylphenyl)-indazole]. These findings may have an important pharmacogenetic effect on the development of new PAR antagonists. PMID:25293779

  9. The polarity protein Par6 is coupled to the microtubule network during molluscan early embryogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Homma, Taihei [Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan); Shimizu, Miho [Kuroda Chiromorphology Team, ERATO-SORST, JST, Komaba, Meguro-ku, Tokyo 153-8902 (Japan); Kuroda, Reiko, E-mail: ckuroda@mail.ecc.u-tokyo.ac.jp [Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan); Kuroda Chiromorphology Team, ERATO-SORST, JST, Komaba, Meguro-ku, Tokyo 153-8902 (Japan); Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902 (Japan)

    2011-01-07

    Research highlights: {yields} The cDNAs encoding Par6 and aPKC homologues were cloned from the snail Lymnaea stagnalis. {yields} L. stagnalis Par6 directly interacts with tubulin and microtubules and localizes to the microtubule cytoskeleton during the early embryogenesis. {yields} Identical sequence and localization of LsPar6 for the dextral and the sinistral snails exclude the possibility of the gene being the primary determinant of body handedness. -- Abstract: Cell polarity, which directs the orientation of asymmetric cell division and segregation of fate determinants, is a fundamental feature of development and differentiation. Regulators of polarity have been extensively studied, and the critical importance of the Par (partitioning-defective) complex as the polarity machinery is now recognized in a wide range of eukaryotic systems. The Par polarity module is evolutionarily conserved, but its mechanism and cooperating factors vary among different systems. Here we describe the cloning and characterization of a pond snail Lymnaea stagnalis homologue of partitioning-defective 6 (Lspar6). The protein product LsPar6 shows high affinity for microtubules and localizes to the mitotic apparatus during embryonic cell division. In vitro assays revealed direct binding of LsPar6 to tubulin and microtubules, which is the first evidence of the direct interaction between the two proteins. The interaction is mediated by two distinct regions of LsPar6 both located in the N-terminal half. Atypical PKC, a functional partner of Par6, was also found to localize to the mitotic spindle. These results suggest that the L. stagnalis Par complex employs the microtubule network in cell polarity processes during the early embryogenesis. Identical sequence and localization of LsPar6 for the dextral and the sinistral snails exclude the possibility of the gene being the primary determinant of handedness.

  10. Mapping transmembrane residues of proteinase activated receptor 2 (PAR2) that influence ligand-modulated calcium signaling.

    Science.gov (United States)

    Suen, J Y; Adams, M N; Lim, J; Madala, P K; Xu, W; Cotterell, A J; He, Y; Yau, M K; Hooper, J D; Fairlie, D P

    2017-03-01

    Proteinase-activated receptor 2 (PAR2) is a G protein-coupled receptor involved in metabolism, inflammation, and cancers. It is activated by proteolysis, which exposes a nascent N-terminal sequence that becomes a tethered agonist. Short synthetic peptides corresponding to this sequence also activate PAR2, while small organic molecules show promising PAR2 antagonism. Developing PAR2 ligands into pharmaceuticals is hindered by a lack of knowledge of how synthetic ligands interact with and differentially modulate PAR2. Guided by PAR2 homology modeling and ligand docking based on bovine rhodopsin, followed by cross-checking with newer PAR2 models based on ORL-1 and PAR1, site-directed mutagenesis of PAR2 was used to investigate the pharmacology of three agonists (two synthetic agonists and trypsin-exposed tethered ligand) and one antagonist for modulation of PAR2 signaling. Effects of 28 PAR2 mutations were examined for PAR2-mediated calcium mobilization and key mutants were selected for measuring ligand binding. Nineteen of twenty-eight PAR2 mutations reduced the potency of at least one ligand by >10-fold. Key residues mapped predominantly to a cluster in the transmembrane (TM) domains of PAR2, differentially influence intracellular Ca(2+) induced by synthetic agonists versus a native agonist, and highlight subtly different TM residues involved in receptor activation. This is the first evidence highlighting the importance of the PAR2 TM regions for receptor activation by synthetic PAR2 agonists and antagonists. The trypsin-cleaved N-terminus that activates PAR2 was unaffected by residues that affected synthetic peptides, challenging the widespread practice of substituting peptides for proteases to characterize PAR2 physiology.

  11. Hemangioma capilar de la parótida. Abordaje transoral

    Directory of Open Access Journals (Sweden)

    Luis Domingo Sarra

    2014-10-01

    Presentamos un caso en una paciente adulta que consultó por una masa en la región parotídea y parálisis facial periférica con el mayor componente tumoral que se manifestaba en la cavidad oral. La ecografía mostró una masa sólida. La punción aspiración con aguja fina fue negativa con material hemático en 2 oportunidades. No tuvimos sospecha de la presencia de un tumor de origen vascular, por lo que no fue solicitada una RMN. El caso fue resuelto a través de un abordaje quirúrgico poco utilizado, tras obtener una biopsia por congelación negativa.

  12. Optimisation de contrôleurs par essaim particulaire

    OpenAIRE

    Fix, Jérémy; Geist, Matthieu

    2012-01-01

    http://cap2012.loria.fr/pub/Papers/10.pdf; National audience; Trouver des contrôleurs optimaux pour des systèmes stochastiques est un problème particulièrement difficile abordé dans les communautés d'apprentissage par renforcement et de contrôle optimal. Le paradigme classique employé pour résoudre ces problèmes est celui des processus décisionnel de Markov. Néanmoins, le problème d'optimisation qui en découle peut être difficile à résoudre. Dans ce papier, nous explorons l'utilisation de l'o...

  13. Cancro em mogno africano no estado do Pará

    Directory of Open Access Journals (Sweden)

    Célia Regina Tremacoldi

    2013-06-01

    Full Text Available Sintomas de cancro foram observados em plantios de mogno africano, no município de Dom Eliseu, Pará. Os fungos associados aos tecidos sintomáticos foram identificados como Lasiodiplodia theobromae e Fusarium subglutinans. Culturas puras desses fungos foram testadas quanto à patogenicidade em mudas de mogno africano sadias. Apenas L. theobromae produziu cancro, por meio de inoculação por ferimento e alta umidade. A confirmação de L. theobromae como agente causal do cancro ocorreu após o reisolamento do fungo a partir das plantas inoculadas em casa de vegetação, concluindo a primeira verificação do postulado de Koch para esse patossistema.

  14. Parálisis cerebral. Tratamiento ortopodológico

    OpenAIRE

    Querol Martínez, Esther; Crespo Martínez, Artur; Concustell Gonfaus, Josep; Sacristan Valero, Sergi; Concustell Fargas, Olga; Carrera Casanova, Ana

    2005-01-01

    Después de varios años de experiencia en la aplicación de las férulas estabilizadoras del sistema aquileocalcáneo plantar FESAP) en pacientes con parálisis cerebral, creemos que deberían ser el tratamiento ortopodológico de elección del pie equino en niños con miopatías y lesiones neurológlcas de la unidad motora, del sistema piramidal o extrapiramidal, tanto por su perfecta adaptación a la morfología de la extremidad inferior como por sus caracteristicas funcionales y sus acciones fisio1ógic...

  15. Usinage des Composites par Jet d'eau Abrasif

    OpenAIRE

    Cénac, François; Collombet, Francis; Zitoune, Rédouane; Déléris, Michel

    2011-01-01

    International audience; Le protocole expérimental de fraisage par jet d'eau abrasif obtenu sur aluminium est adapté aux matériaux composites. On met en exergue deux différences majeures liées à l'effet du JEA entre les composites et l'aluminium que sont l'usinabilité et les variabilités. L'usinabilité des matériaux composites étant plus grande que celle de l'aluminium pour le JEA, on propose des plages de paramètres différentes pour les composites, du fait des limitations des machines standar...

  16. IMPROVEMENT OF PRODUCTION PLANNING IN COMPANY PARS KOMPONENTY

    Directory of Open Access Journals (Sweden)

    René Klega

    2014-03-01

    Full Text Available The article analyzes the current status of production planning in company Pars Komponenty s.r.o., proposes a new method of planning based on application of the principle of MRP. It is a discrete type of production with high complexity of BOM and MTO (Make-to-Order and ETO (Engineering-to-Order from the point of decoupling point. The original planning system plans according to production capacity backward without collisions, but for a given type of production does not work in practice. Planning system was analyzed and the main problems were identified, which were high work in progress and material stocks. This article target is to propose a new planining system based on the inclusion of time reserves of purchased material items. New planning system was tested in practise with benefit in reducing both the material inventory and work in progress.

  17. Pierre Vilar Frontinhan 1906 - París 2003

    Directory of Open Access Journals (Sweden)

    Pablo Rodríguez

    2010-08-01

    Full Text Available En el pasado mes de agosto falleció en París el historiador y maestro de tantas generaciones Pierre Vilar. Aunque había mantenido una intensa actividad de reflexión sobre los sucesos contemporáneos, ésta se vio afectada en 1991 luego que una grave enfermedad le negó la visión. Fue entonces cuando decidió con la ayuda de Rosa Congost escribir sus reflexiones y recuerdos, especie de memoria de juventud. Pensar Históricamente (Barcelona: Editorial Crítica, 1997, el libro que reúne estos ejercicios, es una obra de sumo interés para conocer la infancia, adolescencia y juventud de este connotado historiador.

  18. Clinical outcomes of pars plicata anterior vitrectomy: 2-year results

    Directory of Open Access Journals (Sweden)

    Priya Narang

    2015-01-01

    Full Text Available Purpose: To demonstrate the safety and outcome of a surgical approach that uses pars plicata site for anterior vitrectomy during phacoemulsification procedure complicated by posterior capsule rupture and residual cortical matter. Design: Single center, retrospective, interventional, noncomparative study. Materials and Methods: Medical records of a consecutive series of 35 eyes of 35 patients who underwent pars plicata anterior vitrectomy (PPAV were reviewed. The main outcome measures were corrected and uncorrected distance visual acuity (CDVA, UDVA, early and late postoperative complications and intraocular pressure (IOP. Ultrasound biomicroscopic (UBM evaluation of sclerotomy site and spectral domain optical coherence tomography analysis for central macular thickness (CMT was performed. The final visual outcome at 2 years was evaluated. Results: At 2 years follow-up, the mean postoperative UDVA (logarithm of the minimum angle of resolution [logMAR] and CDVA (logMAR was 0.49 ± 0.26 and 0.19 ± 0.14, respectively. There was no significant change in the IOP (P = 0.061 and the mean CMT at 2 years was 192.5 ± 5.54 mm. The postoperative UBM image of the sclerotomy site at 8 weeks demonstrated a clear wound without any vitreous adhesion or incarceration. Intraoperative hyphema was seen in 1 (2.8% case and postoperative uveitis was seen in 2 (5.7% cases, which resolved with medications. No case of an iatrogenic retinal break or retinal detachment was reported. Conclusions: PPAV enables a closed chamber approach, allows thorough cleanup of vitreous in the pupillary plane and anterior chamber and affords better access to the subincisional and retropupillary cortical remnant with a significant visual outcome and an acceptable complication rate.

  19. Entrenamiento deportivo en relación con la función motora gruesa y la independencia funcional en niños y jóvenes con parálisis cerebral pertenecientes a la selección Valle de Boccias

    OpenAIRE

    Ortega Arellano, Camilo Ernesto; Ramon Cuellar, Marcela

    2010-01-01

    Tesis (Maestría en Desarrollo Infantil). Universidad de Manizales. Facultad de Ciencias Sociales y Humanas, 2010 Se realizo un estudio intrasujeto pre y post prueba con grupo control, con una muestra de 10 niños y jóvenes con parálisis cerebral pertenecientes a la selección Valle de Boccia y un grupo control de 10 niños y jóvenes con parálisis cerebral y condiciones motoras similares, con el objetivo de describir los efectos del entrenamiento deportivo en los niños y jóvenes con parálisis...

  20. Parálisis cerebral y sistemas de seguimiento de la mirada: ¿clic por parpadeo o permanencia?

    OpenAIRE

    Clemotte, Alejandro; Velasco, Miguel; Raya, Rafael; Ceres, Ramón; Córdoba Herralde, Ricardo de

    2015-01-01

    Los comandos básicos para el control de ordenadores a través de interfaces gráficas son comandos de alcance a un objeto en pantalla y su selección. El presente documento describe un estudio comparativo a nivel funcional de dos estrategias de selección alternativas, especialmente diseñada para personas con parálisis cerebral: clic por permanencia y clic por parpadeo, mientras que la tarea de alcance se realiza a través de movimientos oculares.

  1. Effect of pre-drying and par-frying conditions on the crispness of French fries

    NARCIS (Netherlands)

    Loon, W.A.M.; Visser, J.E.; Linssen, J.P.H.; Somsen, D.J.; Klok, H.J.; Voragen, A.G.J.

    2007-01-01

    An experimental design was used to study the effect of pre-drying (to 10, 15 and 20% weight loss) and par-frying conditions (160, 170 and 180 °C) on the crispness of French fries. Par-frying time was adjusted with a software program to obtain equal moisture content and internal texture for all sampl

  2. 12 CFR 925.19 - Par value and price of stock.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Par value and price of stock. 925.19 Section 925.19 Banks and Banking FEDERAL HOUSING FINANCE BOARD FEDERAL HOME LOAN BANK MEMBERS AND HOUSING ASSOCIATES MEMBERS OF THE BANKS Stock Requirements § 925.19 Par value and price of stock. The capital...

  3. Comparison of Extrapolation and Interpolation Methods for Estimating Daily Photosynthetically Active Radiation (PAR)

    NARCIS (Netherlands)

    Wu, G.; Skidmore, A.K.; Leeuw, de J.; Liu, X.; Prins, H.H.T.

    2010-01-01

    Measurements of photosynthetically active radiation (PAR), which are indispensable for simulating plant growth and productivity, are generally very scarce. This study aimed to compare two extrapolation and one interpolation methods for estimating daily PAR reaching the earth surface within the Poyan

  4. Elevated soluble urokinase plasminogen activator receptor (suPAR) predicts mortality in Staphylococcus aureus bacteremia

    DEFF Research Database (Denmark)

    Mölkänen, T; Ruotsalainen, E; Thorball, C W

    2011-01-01

    are scarce. To elucidate the role of suPAR in a common bacteremic infection, the serum suPAR levels in 59 patients with Staphylococcus aureus bacteremia (SAB) were measured using the suPARnostic ELISA assay and associations to 1-month mortality and with deep infection focus were analyzed. On day three, after...

  5. A deficiency of uPAR alters endothelial angiogenic function and cell morphology

    Directory of Open Access Journals (Sweden)

    Balsara Rashna D

    2011-05-01

    Full Text Available Abstract The angiogenic potential of a cell requires dynamic reorganization of the cytoskeletal architecture that involves the interaction of urokinase-type plasminogen activator receptor (uPAR with the extracellular matrix. This study focuses on the effect of uPAR deficiency (uPAR-/- on angiogenic function and associated cytoskeletal organization. Utilizing murine endothelial cells, it was observed that adhesion, migration, proliferation, and capillary tube formation were altered in uPAR-/- cells compared to wild-type (WT cells. On a vitronectin (Vn matrix, uPAR-/- cells acquired a "fried egg" morphology characterized by circular actin organization and lack of lamellipodia formation. The up-regulation of β1 integrin, FAK(P-Tyr925, and paxillin (P-Tyr118, and decreased Rac1 activation, suggested increased focal adhesions, but delayed focal adhesion turnover in uPAR-/- cells. This accounted for the enhanced adhesion, but attenuated migration, on Vn. VEGF-enriched Matrigel implants from uPAR-/- mice demonstrated a lack of mature vessel formation compared to WT mice. Collectively, these results indicate that a uPAR deficiency leads to decreased angiogenic functions of endothelial cells.

  6. A Bipolar Spindle of Antiparallel ParM Filaments Drives Bacterial Plasmid Segregation

    DEFF Research Database (Denmark)

    Gayathri, P; Fujii, T; Møller-Jensen, Jakob;

    2012-01-01

    To ensure their stable inheritance by daughter cells during cell division, bacterial low copy-number plasmids make simple DNA segregating machines that use an elongating protein filament between sister plasmids. In the ParMRC system of Escherichia coli R1 plasmid, ParM, an actin-like protein, forms...

  7. Compression Myelopathy due to Proliferative Changes around C2 Pars Defects without Instability

    Science.gov (United States)

    Kimura, Tetsuya; Tezuka, Fumitake; Abe, Mitsunobu; Yamashita, Kazuta; Takata, Yoichiro; Higashino, Kosaku; Sairyo, Koichi

    2016-01-01

    We report a case with compression myelopathy due to proliferative changes around the C2 pars defects without instability. A 69-year-old man presented with progressive clumsy hands and spastic gait. Plain radiographs showed bilateral spondylolysis (pars defects) at C2 and fusion between C2 and C3 spinous processes. Dynamic views revealed mobility through the pars defects, but there was no apparent instability. Computed tomography showed proliferative changes at the pars defects, which protruded into spinal canal. On magnetic resonance imaging, the spinal cord was compressed and intramedullary high signal change was found. A diagnosis of compression myelopathy due to proliferative changes around the C2 pars defects was made. We performed posterior decompression. Postoperatively, symptoms have been alleviated and images revealed sufficient decompression and no apparent instability. In patients with the cervical spondylolysis, myelopathy caused by instability or slippage have been periodically reported. The present case involving C2 spondylolysis is extremely rare. PMID:27340539

  8. Brulure par Plaque de Bistouri Electrique: a Propos de Quatre Cas

    OpenAIRE

    Khales, A.; Achbouk, A.; Belmir, R.; Cherkab, L.; M.A. Ennouhi; Ababou, K.; Ihrai, H.

    2010-01-01

    La brûlure par plaque de bistouri électrique est un accident rare mais grave par la profondeur de la lésion et par sa localisation, surtout quand qu’elle survient dans un contexte chirurgical dont le vécu reste difficile de la part du malade et du chirurgien. Cette brûlure bien que imprévisible reste grave par la profondeur et la localisation de la brûlure et par sa survenue dans un contexte opératoire, chez des patients malades. La prise en charge de la brûlure doit se faire en milieu spécia...

  9. PAR Interception and Utilization in Different Maize and Soybean Intercropping Patterns

    Science.gov (United States)

    Liu, Xin; Rahman, Tanzeelur; Yang, Feng; Song, Chun; Yong, Taiwen; Liu, Jiang; Zhang, Cuiying; Yang, Wenyu

    2017-01-01

    The crop intercepted photosynthetically active radiation (PAR) and radiation use efficiency (RUE) vary markedly in different intercropping systems. The HHLA (horizontally homogeneous leaf area) and ERCRT (extended row crop radiation transmission) models have been established to calculate the intercepted PAR for intercrops. However, there is still a lack of study on the intercepted PAR and RUE under different intercropping configurations using different models. To evaluate the intercepted PAR and RUE in maize and soybean under different intercropping systems, we tested different strip intercropping configurations (SI1, SI2, and SI3 based on ERCRT model) and a row intercropping configurations (RI based on HHLA model) in comparison to monoculture. Our results showed that the intercepted PAR and RUE of intercropping systems were all higher than those of monoculture. The soybean intercepted PAR in strip intercropping was 1.35 times greater than that in row intercropping. In row intercropping (RI), the lack of soybean intercepted PAR resulted in a significant reduction of soybean dry matter. Therefore, it is not the recommended configuration for soybean. In strip intercropping patterns, with the distance between maize strip increased by 0.2 m, the intercepted PAR of soybean increased by 20%. The SI2 (maize row spacing at 0.4 m and the distance between maize strip at 1.6 m) was the recommended configuration to achieve the highest value of intercepted PAR and RUE among tested strip intercropping configurations. The method of dry matter estimation using intercepted PAR and RUE is useful in simulated experiments. The simulated value was verified in comparison with experimental data, which confirmed the credibility of the simulation model. Moreover, it also provides help in the development of functional-structural plant model (FSPM). PMID:28056056

  10. Facteurs de risque de mortalité par tuberculose pulmonaire

    Science.gov (United States)

    Janah, Hicham; Souhi, Hicham; Kouismi, Hatim; Mark, Karima; Zahraoui, Rachida; Benamor, Jouda; Soualhi, Mona; Bourkadi, Jamal Eddine

    2014-01-01

    La tuberculose est une maladie infectieuse transmissible provoquée par myco-bacterium tuberculosis (bacille de Koch ou BK). Elle représente, selon les estimations del'Organisation Mondiale de la Santé (OMS), l'une des pathologies infectieuses causant le plus de décès au niveau mondial avec plus de 1 million de décès par an. Pour déterminer les facteurs de risque de mortalité au cours de la tuberculose pulmonaire à microscopie positive nous avons mené une étude rétrospective portant sur tous les cas de tuberculose pulmonaire à microscopie positive et qui étaient décédés au cours de leur hospitalisation. Cette étude a colligé 1803 cas de tuberculose sur une période de 2 ans et demi dont 46 sont décédés. La prévalence de décès est de 2,55%. La population se répartit en 32 hommes et 14 femmes. L’âge moyen était de 53ans ± 17 ans. Le tabagisme était retrouvé chez la moitié des cas. Une comorbidité était retrouvée dans 43%, avec 17% de diabète. Le délai de diagnostic avait une médiane de 60 jours avec percentile (30j; 105j). La symptomatologie clinique était dominée par la toux, la dyspnée et les expectorations soit respectivement: 97,8%, 69,6% et 67,4% des cas. Sur le plan radiologique les lésions étaient diffuses et bilatérales dans 76,1% des cas. Tous les patients étaient mis sous SRHZ. 11% avaient présenté une toxicité aux antibacillaires (de type hépatiques dans 3 cas et neurologiques dans 2 cas). Le délai médian de décès était de 8,5 jours (5j; 17j). Les causes de décès retrouvées étaient: Une hépatite fulminante (3 cas), une décompensation acido-cétosique (3 cas), un SDRA (2 cas), des hémoptysies foudroyantes (2 cas), et respectivement un cas secondaire à une décompensation de BPCO, une décompensation cardiaque, une hypoglycémie et un tableau d'anasarque. Cette étude suggère que le terrain, le retard diagnostique et les effets secondaires du traitement sont les principaux facteurs de risque de

  11. Elastic and inelastic scattering of 2 to 10 MeV protons by lithium isotopes; Diffusion elastique et inelastique des protons de 2 a 10 MeV par les isotopes du lithium

    Energy Technology Data Exchange (ETDEWEB)

    Laurat, M. [Commissariat a l' Energie Atomique, Bruyeres-le-Chatel (France). Centre d' Etudes

    1969-07-01

    A description is given of the experimental set-up which has been devised for carrying out spectrometric and absolute cross-section measurements on the reactions induced by protons accelerated in a 12 MeV Van de Graaff Tandem. The particles are detected by silicon junctions; the weight of the targets (about ten {mu}g/cm{sup 2}) is determined by the quartz method. The experimental equipment has been controlled by a study of proton scattering by lithium-6, and has made it possible to evaluate the elastic and inelastic scattering (1. level excitation) by lithium 7 of 2 to 9 MeV protons. The most probable spin and parity values for the six levels of {sup 8}Be between 19 and 25 MeV excitation energy have been determined from a knowledge of the observed structure. (author) [French] Nous decrivons le dispositif experimental mis au point pour effectuer les mesures de spectrometrie et de section efficace absolue pour les reactions induites par des protons acceleres par un Van de Graaff Tandem 12 MeV. Les particules sont detectees par des jonctions au silicium, le poids des cibles (de l'ordre d'une dizaine de {mu}g/cm{sup 2}), mesure par la methode du quartz. L'ensemble de l'appareillage a ete controle par l'etude de la diffusion des protons par le lithium 6, et nous a permis de preciser les diffusions elastiques et inelastiques (excitation du 1er niveau) des protons de 2 a 9 MeV par le lithium 7. La structure observee a permis de determiner les spin et parite les plus probables de six niveaux du {sup 8}Be entre 19 et 25 MeV d'energie d'excitation. (auteur)

  12. La limitation de l’ontologie par la logique

    Directory of Open Access Journals (Sweden)

    John J. Drummond

    2009-02-01

    Full Text Available Cet article maintient que l’intérêt de Husserl pour le développement d’une logique pure en tant que théorie de la science limite sa conception de l’ontologie. L’ontologie formelle est, pour Husserl, une théorie formelle des objets de connaissance, dont les catégories fondamentales sont celles de substance, propriété et relation. En outre, les ontologies régionales évoluent au sein des limites catégorielles définies par l’ontologie formelle. Mais une telle ontologie laisse de côté les activités et les processus de tout genre, parmi lesquels le plus important est celui de l’ « avoir à l’esprit ». La phénoménologie transcendantale, en revanche, s’occupe de l’être-conscient-du-monde, un être-conscient qui est cependant inséparable de l’être-dans-le-monde. Cette phénoménologie se situe sur le même terrain ontologique que l’ontologie fondamentale de Heidegger. Mais alors, bien que la notion husserlienne d’ontologie soit limitée par le rôle dominant attribué à la logique en tant que théorie de la science et à la connaissance vraie qui lui est propre, il y a plus d’ontologie chez Husserl que dans son « ontologie ».This paper argues that Husserl’s concern with the development of pure logic as a theory of science limits his conception of ontology. Formal ontology is, for Husserl, a formal theory of the objects of cognition, and its fundamental categories are substantiality, property, and relation. Moreover, regional ontologies work within the categorial boundaries defined by formal ontology. Lost from view in this ontology are activities and processes of various kinds, of which “minding” is the most significant. Husserl’s transcendental phenomenology is an account of being-conscious-of-the-world, a being-conscious, however, that is inseparable from being-in-a-world.  This phenomenology is aimed at the same ontological terrain as Heidegger’s fundamental ontology. Hence, while

  13. Measurement of cortisol concentration in the tears of horses and ponies with pituitary pars intermedia dysfunction.

    Science.gov (United States)

    Hart, Kelsey A; Kitchings, Kalyn M; Kimura, Shune; Norton, Natalie A; Myrna, Kathern E

    2016-11-01

    OBJECTIVE To compare tear cortisol concentrations between horses and ponies with pituitary pars intermedia dysfunction (PPID) and healthy nonaged (≤ 15 years old) and aged (≥ 20 years old) horses and to determine whether serum and tear cortisol concentrations were correlated. ANIMALS 11 horses and ponies with PPID and 20 healthy control horses and ponies (11 nonaged and 9 aged). PROCEDURES Paired tear and serum samples were obtained from PPID and control animals. All animals were free of active ocular disease. Tear and serum cortisol concentrations were measured with an ELISA and chemiluminescent assay, respectively. Groups were compared with Kruskal-Wallis and Mann-Whitney U tests, and Spearman correlation analysis was used to examine relationships between tear and serum cortisol concentrations within groups. RESULTS Median tear cortisol concentration was significantly higher in PPID animals than in aged control animals, despite comparable serum cortisol concentrations in PPID and aged control animals. Median tear-to-serum cortisol concentration ratios were also significantly higher in PPID animals than in aged control animals. Serum and tear cortisol concentrations were not significantly correlated in PPID or control animals. CONCLUSIONS AND CLINICAL RELEVANCE Some horses and ponies with PPID had increased tear cortisol concentrations, compared with concentrations in healthy aged animals. Localized cortisol production in the tear film or altered cortisol binding dynamics could have contributed to this increase. Further studies are warranted to evaluate these mechanisms and to determine whether increased tear cortisol concentrations are associated with delays in corneal wound healing in horses and ponies with and without PPID.

  14. Profiling gene expression induced by protease-activated receptor 2 (PAR2 activation in human kidney cells.

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    Jacky Y Suen

    Full Text Available Protease-Activated Receptor-2 (PAR2 has been implicated through genetic knockout mice with cytokine regulation and arthritis development. Many studies have associated PAR2 with inflammatory conditions (arthritis, airways inflammation, IBD and key events in tumor progression (angiogenesis, metastasis, but they have relied heavily on the use of single agonists to identify physiological roles for PAR2. However such probes are now known not to be highly selective for PAR2, and thus precisely what PAR2 does and what mechanisms of downstream regulation are truly affected remain obscure. Effects of PAR2 activation on gene expression in Human Embryonic Kidney cells (HEK293, a commonly studied cell line in PAR2 research, were investigated here by comparing 19,000 human genes for intersecting up- or down-regulation by both trypsin (an endogenous protease that activates PAR2 and a PAR2 activating hexapeptide (2f-LIGRLO-NH(2. Among 2,500 human genes regulated similarly by both agonists, there were clear associations between PAR2 activation and cellular metabolism (1,000 genes, the cell cycle, the MAPK pathway, HDAC and sirtuin enzymes, inflammatory cytokines, and anti-complement function. PAR-2 activation up-regulated four genes more than 5 fold (DUSP6, WWOX, AREG, SERPINB2 and down-regulated another six genes more than 3 fold (TXNIP, RARG, ITGB4, CTSD, MSC and TM4SF15. Both PAR2 and PAR1 activation resulted in up-regulated expression of several genes (CD44, FOSL1, TNFRSF12A, RAB3A, COPEB, CORO1C, THBS1, SDC4 known to be important in cancer. This is the first widespread profiling of specific activation of PAR2 and provides a valuable platform for better understanding key mechanistic roles of PAR2 in human physiology. Results clearly support the development of both antagonists and agonists of human PAR2 as potential disease modifying therapeutic agents.

  15. Proteinase-Activated Receptor-1 and Immunomodulatory Effects of a PAR1-Activating Peptide in a Mouse Model of Prostatitis

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    M. Mark Stanton

    2013-01-01

    Full Text Available Background. Nonbacterial prostatitis has no established etiology. We hypothesized that proteinase-activated receptor-1 (PAR1 can play a role in prostatitis. We therefore investigated the effects of PAR1 stimulation in the context of a new model of murine nonbacterial prostatitis. Methods. Using a hapten (ethanol-dinitrobenzene sulfonic acid- (DNBS- induced prostatitis model with both wild-type and PAR1-null mice, we examined (1 the location of PAR1 in the mouse prostate and (2 the impact of a PAR1-activating peptide (TFLLR-NH2: PAR1-TF on ethanol-DNBS-induced inflammation. Results. Ethanol-DNBS-induced inflammation was maximal at 2 days. In the tissue, PAR1 was expressed predominantly along the apical acini of prostatic epithelium. Although PAR1-TF on its own did not cause inflammation, its coadministration with ethanol-DNBS reduced all indices of acute prostatitis. Further, PAR1-TF administration doubled the prostatic production of interleukin-10 (IL-10 compared with ethanol-DNBS treatment alone. This enhanced IL-10 was not observed in PAR1-null mice and was not caused by the reverse-sequence receptor-inactive peptide, RLLFT-NH2. Surprisingly, PAR1-TF, also diminished ethanol-DNBS-induced inflammation in PAR1-null mice. Conclusions. PAR1 is expressed in the mouse prostate and its activation by PAR1-TF elicits immunomodulatory effects during ethanol-DNBS-induced prostatitis. However, PAR1-TF also diminishes ethanol-DNBS-induced inflammation via a non-PAR1 mechanism by activating an as-yet unknown receptor.

  16. Proteinase-activated receptor-1 and immunomodulatory effects of a PAR1-activating peptide in a mouse model of prostatitis.

    Science.gov (United States)

    Stanton, M Mark; Nelson, Lisa K; Benediktsson, Hallgrimur; Hollenberg, Morley D; Buret, Andre G; Ceri, Howard

    2013-01-01

    Nonbacterial prostatitis has no established etiology. We hypothesized that proteinase-activated receptor-1 (PAR1) can play a role in prostatitis. We therefore investigated the effects of PAR1 stimulation in the context of a new model of murine nonbacterial prostatitis. Using a hapten (ethanol-dinitrobenzene sulfonic acid- (DNBS-)) induced prostatitis model with both wild-type and PAR1-null mice, we examined (1) the location of PAR1 in the mouse prostate and (2) the impact of a PAR1-activating peptide (TFLLR-NH2: PAR1-TF) on ethanol-DNBS-induced inflammation. Ethanol-DNBS-induced inflammation was maximal at 2 days. In the tissue, PAR1 was expressed predominantly along the apical acini of prostatic epithelium. Although PAR1-TF on its own did not cause inflammation, its coadministration with ethanol-DNBS reduced all indices of acute prostatitis. Further, PAR1-TF administration doubled the prostatic production of interleukin-10 (IL-10) compared with ethanol-DNBS treatment alone. This enhanced IL-10 was not observed in PAR1-null mice and was not caused by the reverse-sequence receptor-inactive peptide, RLLFT-NH2. Surprisingly, PAR1-TF, also diminished ethanol-DNBS-induced inflammation in PAR1-null mice. PAR1 is expressed in the mouse prostate and its activation by PAR1-TF elicits immunomodulatory effects during ethanol-DNBS-induced prostatitis. However, PAR1-TF also diminishes ethanol-DNBS-induced inflammation via a non-PAR1 mechanism by activating an as-yet unknown receptor.

  17. Analyse numérique du problème de déplacement du noyau fondu en soudage par points de tôles à différentes épaisseurs

    Science.gov (United States)

    Benkedda, Y.; Tahar Chaouch, K.; Ibrir, B.

    2005-05-01

    Le soudage par résistance par points est une technique d'assemblage très répandue, en particulier dans l'industrie automobile, parce qu'elle est rapide, fiable et bon marché. Pour cela, la réalisation d'un point de soudure de qualité reste encore une préoccupation de l'industrie, surtout lorsqu'il s'agit de souder des tôles à différentes épaisseurs. Les premiers modèles mathématiques et expérimentaux qui couvrent ces différents aspects, sont principalement dirigés vers les modèles de transfert de chaleur ou vers les phénomènes de surfaces (modèles de surfaces) ; ces derniers, sont un problème d'importance, puisqu'ils interviennent chaque fois qu'un transfert de chaleur s'effectue entre deux solides accolés. Les physiciens ont étudie expérimentalement la réponse de la région de contact de deux solides aux pulsations de courant. Ils ont montré que le contact possède une résistance de resserrement due aux aspérités de surface, et que la température moyenne à l'interface des deux solides sous un chargement uniforme produit un adoucissement local lequel fait augmenter la région de contact. Le but de ce travail est consacré à l'analyse de l'influence des épaisseurs de tôles sur la position du noyau fondu. Expérimentalement, il a été montré que lorsque le rapport des épaisseurs est compris entre 1/1 et 1/10, le noyau fondu se déplace vers la pièce la plus épaisse. Ce phénomène peut s'expliquer à partir d'une étude thermique. Nous proposons donc, de résoudre le problème de transfert thermique transitoire associé au processus de soudage par points moyennant une méthode numérique. Ce modèle est capable de prédire l'évolution du noyau fondu en fonction des paramètres du procédé à savoir l'intensité de soudage et temps de soudage, ainsi que la répartition thermique en chaque point de l'assemblage.

  18. Determinação do ponto de carga zero da bauxita da região nordeste do Pará Determination of the zero point of charge of the northeast of Pará bauxite

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    R. L. S. Pinto

    2012-12-01

    Full Text Available No nordeste do Pará o transporte da polpa de bauxita é através de mineroduto, sendo a viscosidade um parâmetro de controle fundamental para o bombeamento do minério. Este estudo ilustra a influência do pH na reologia da polpa por meio da determinação do ponto de carga zero (pHpcz da bauxita. Foram efetuadas análise granulométrica, microscopia eletrônica de varredura, ensaios potenciométricos testando o cloreto de amônia como eletrólito indiferente e ensaios reológicos em diferentes valores de pH. Os resultados revelaram que o cloreto de amônia pode ser utilizado como eletrólito indiferente para esse tipo de análise e que ocorre a redução da viscosidade em valores de pH distantes do ponto de carga zero.In the northeast of Pará, Brazil, the transport of bauxite pulp is through pipeline, being the viscosity a parameter which can interfere in the pumping. This study illustrates the influence of pH on pulp rheology through of determination of the zero point of charge. Were done particle size analysis, SEM, EDS, potentiometric tests testing ammonia chloride as indifferent electrolyte and rheological tests at different pH values. The results revealed that ammonia chloride can be used as indifferent electrolyte and showed a decrease in viscosity when there is far of the zero point of charge.

  19. PAR1 activation induces rapid changes in glutamate uptake and astrocyte morphology

    Science.gov (United States)

    Sweeney, Amanda M.; Fleming, Kelsey E.; McCauley, John P.; Rodriguez, Marvin F.; Martin, Elliot T.; Sousa, Alioscka A.; Leapman, Richard D.; Scimemi, Annalisa

    2017-01-01

    The G-protein coupled, protease-activated receptor 1 (PAR1) is a membrane protein expressed in astrocytes. Fine astrocytic processes are in tight contact with neurons and blood vessels and shape excitatory synaptic transmission due to their abundant expression of glutamate transporters. PAR1 is proteolytically-activated by bloodstream serine proteases also involved in the formation of blood clots. PAR1 activation has been suggested to play a key role in pathological states like thrombosis, hemostasis and inflammation. What remains unclear is whether PAR1 activation also regulates glutamate uptake in astrocytes and how this shapes excitatory synaptic transmission among neurons. Here we show that, in the mouse hippocampus, PAR1 activation induces a rapid structural re-organization of the neuropil surrounding glutamatergic synapses, which is associated with faster clearance of synaptically-released glutamate from the extracellular space. This effect can be recapitulated using realistic 3D Monte Carlo reaction-diffusion simulations, based on axial scanning transmission electron microscopy (STEM) tomography reconstructions of excitatory synapses. The faster glutamate clearance induced by PAR1 activation leads to short- and long-term changes in excitatory synaptic transmission. Together, these findings identify PAR1 as an important regulator of glutamatergic signaling in the hippocampus and a possible target molecule to limit brain damage during hemorrhagic stroke. PMID:28256580

  20. Par-4/NF-κB Mediates the Apoptosis of Islet β Cells Induced by Glucolipotoxicity

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    Wu QiNan

    2016-01-01

    Full Text Available Apoptosis of islet β cells is a primary pathogenic feature of type 2 diabetes, and ER stress and mitochondrial dysfunction play important roles in this process. Previous research has shown that prostate apoptosis response-4 (Par-4/NF-κB induces cancer cell apoptosis through endoplasmic reticulum (ER stress and mitochondrial dysfunction. However, the mechanism by which Par-4/NF-κB induces islet β cell apoptosis remains unknown. We used a high glucose/palmitate intervention to mimic type 2 diabetes in vitro. We demonstrated that the high glucose/palmitate intervention induced the expression and secretion of Par-4. It also causes increased expression and activation of NF-κB, which induced NIT-1 cell apoptosis and dysfunction. Overexpression of Par-4 potentiates these effects, whereas downregulation of Par-4 attenuates them. Inhibition of NF-κB inhibited the Par-4-induced apoptosis. Furthermore, these effects occurred through the ER stress cell membrane and mitochondrial pathway of apoptosis. Our findings reveal a novel role for Par-4/NF-κB in islet β cell apoptosis and type 2 diabetes.

  1. Characterization and function of human Ly-6/uPAR molecules

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    Hyun Kyung Kong & Jong Hoon Park*

    2012-11-01

    Full Text Available Human Ly-6/uPAR molecules are a superfamily composed oftwo subfamilies; one is the membrane bound proteins with aGPI-anchor and the other are secreted proteins without theGPI-anchor. Ly-6/uPAR molecules have remarkable amino acidhomology through a distinctive 8-10 cysteine-rich domain thatis associated predominantly with O-linked glycans. Thesemolecules are encoded by multiple tightly linked genes locatedon Chr. 8q23, and have a conserved genomic organization.Ly-6/uPAR molecules have an interesting expression patternduring hematopoiesis and on specific tumors indicating thatLy-6/uPAR molecules are associated with development of theimmune system and carcinogenesis. Thus, Ly-6/uPAR moleculesare useful antigens for diagnostic and therapeutic targets.This review summarizes our understanding of human Ly-6/uPAR molecules with regard to molecular structure as well aswhat is known about their function in normal and malignanttissues and suggest Ly-6/uPAR molecules as target antigens forcancer immunotherapy.

  2. Evidence for a DNA-relay mechanism in ParABS-mediated chromosome segregation.

    Science.gov (United States)

    Lim, Hoong Chuin; Surovtsev, Ivan Vladimirovich; Beltran, Bruno Gabriel; Huang, Fang; Bewersdorf, Jörg; Jacobs-Wagner, Christine

    2014-05-23

    The widely conserved ParABS system plays a major role in bacterial chromosome segregation. How the components of this system work together to generate translocation force and directional motion remains uncertain. Here, we combine biochemical approaches, quantitative imaging and mathematical modeling to examine the mechanism by which ParA drives the translocation of the ParB/parS partition complex in Caulobacter crescentus. Our experiments, together with simulations grounded on experimentally-determined biochemical and cellular parameters, suggest a novel 'DNA-relay' mechanism in which the chromosome plays a mechanical function. In this model, DNA-bound ParA-ATP dimers serve as transient tethers that harness the elastic dynamics of the chromosome to relay the partition complex from one DNA region to another across a ParA-ATP dimer gradient. Since ParA-like proteins are implicated in the partitioning of various cytoplasmic cargos, the conservation of their DNA-binding activity suggests that the DNA-relay mechanism may be a general form of intracellular transport in bacteria.DOI: http://dx.doi.org/10.7554/eLife.02758.001.

  3. PAR-1/MARK: a kinase essential for maintaining the dynamic state of microtubules.

    Science.gov (United States)

    Hayashi, Kenji; Suzuki, Atsushi; Ohno, Shigeo

    2012-01-01

    The serine/threonine kinase, PAR-1, is an essential component of the evolutionary-conserved polarity-regulating system, PAR-aPKC system, which plays indispensable roles in establishing asymmetric protein distributions and cell polarity in various biological contexts (Suzuki, A. and Ohno, S. (2006). J. Cell Sci., 119: 979-987; Matenia, D. and Mandelkow, E.M. (2009). Trends Biochem. Sci., 34: 332-342). PAR-1 is also known as MARK, which phosphorylates classical microtubule-associated proteins (MAPs) and detaches MAPs from microtubules (Matenia, D. and Mandelkow, E.M. (2009). Trends Biochem. Sci., 34: 332-342). This MARK activity of PAR-1 suggests its role in microtubule (MT) dynamics, but surprisingly, only few studies have been carried out to address this issue. Here, we summarize our recent study on live imaging analysis of MT dynamics in PAR-1b-depleted cells, which clearly demonstrated the positive role of PAR-1b in maintaining MT dynamics (Hayashi, K., Suzuki, A., Hirai, S., Kurihara, Y., Hoogenraad, C.C., and Ohno, S. (2011). J. Neurosci., 31: 12094-12103). Importantly, our results further revealed the novel physiological function of PAR-1b in maintaining dendritic spine morphology in mature neurons.

  4. Common variants in the human platelet PAR4 thrombin receptor alter platelet function and differ by race

    OpenAIRE

    Edelstein, Leonard C.; Simon, Lukas M.; Lindsay, Cory R.; Kong, Xianguo; Teruel-Montoya, Raúl; Tourdot, Benjamin E.; Chen, Edward S.; Ma,Lin; Coughlin, Shaun; Nieman, Marvin; Holinstat, Michael; Shaw, Chad A.; Bray, Paul F.

    2014-01-01

    White individuals have a high frequency of the common PAR4 gene (F2RL3) variant Ala120; blacks have a high frequency of Thr120.PAR4 Thr120 induces greater signaling and is associated with greater platelet aggregation and reduced inhibition by a PAR4 antagonist.

  5. Hunger amidst plenty along the coast of Pará

    Directory of Open Access Journals (Sweden)

    Ivete Nascimento

    2006-08-01

    Full Text Available In this work one analyzes some referring questions to the new significações of the temporality constructed socially enters the artisan fishing of the coast, in the city of Maracanã, using as boundary-maker the concepts the concepts of abundance and famitura the one that they send themselves to the speech in way of life of the past and the gift. It will see as the typical way of life of the rescued past of the memory of old and the meaning of the abundance in the time of the fishing-farmer, the counterpoint with the concept of famitura of the fishing-exclusive of the gift, where the loss surpasses the scope of the strict survival reaching the proper way of life with the weakness of solidarity. This analysis will be given mecanicamente in the conceptual field of the time measured for the tasks and of the time chronometered for the clock. The intensive field work ocurred on Fortaleza of the Mocooca community incorporating data gotten a long the author's experience in the fish artisan thematic of the Pará coast.

  6. Triamcinolone-assisted Pars Plana Vitrectomy for Retinal Disease

    Institute of Scientific and Technical Information of China (English)

    Yuping Zheng; Naixue Sun; Quanchen Xiong; Xiaohua Wang; Yanlong Quan

    2005-01-01

    Purpose:To determine whether triamcinolone acetonide (TA)staining facilitates posterior hyaloid removal in patients undergoing pars plana vitrectomy (PPV) for retinal disease.Methods: A triamcinolone acetonide(TA)-assisted vitrectomy was performed on patients with the following disease: proliferative diabetic retionpathy(5eyes), central retinal vein occlusion(5eyes), macuar hole (3eyes), and epiretinal membrane(2eyes). Eyes without apparent preoperative posterior vitreous detachment were enrolled in this study. After a core PPV, TA aqueous suspension (40 mg/ml) was injected into the mid vitreous cavity to visualize the posterior hyaloid, thus allowing a complete posterior hyaloid separation and removal. The visual acuity, intraocular pressure (IOP), tamponade, corneal pathology, after-cataract, vitreous hemorrhage, and necessity for reoperation were thereafter examined for at least 3 months after surgery.Results: In all patients, the vitreous body was clearly seen by means of triamcinolone during surgery, and complete removal of posterior hyaloid was facilitated and confirmed.Retina was attached in 14 of 15 eyes, and vision acuity was improved in 9 of 15 eyes.Two eyes showed transient postoperative IOP elevation, 2 eyes had after cataract formation and 1eye had cataract progression. Vitreous hemorrhage occurred in 1 eye. No eye had corneal pathology.Conclusion: Triamcinolone improved the visibility of the hyaloid and the safety of surgical procedures during PPV. No obvious adverse effect due to toxicity of TA accrued in TA-assisted PPV.

  7. Photometric monitoring of the young star Par 1724 in Orion

    Science.gov (United States)

    Neuhäuser, R.; Koeltzsch, A.; Raetz, St.; Schmidt, T. O. B.; Mugrauer, M.; Young, N.; Bertoldi, F.; Roell, T.; Eisenbeiss, T.; Hohle, M. M.; Vaňko, M.; Ginski, C.; Rammo, W.; Moualla, M.; Broeg, C.

    2009-05-01

    We report new photometric observations of the ˜ 200 000 year old naked weak-line run-away T Tauri star Par 1724, located north of the Trapezium cluster in Orion. We observed in the broad band filters B, V, R, and I using the 90 cm Dutch telescope on La Silla, the 80 cm Wendelstein telescope, and a 25 cm telescope of the University Observatory Jena in Großschwabhausen near Jena. The photometric data in V and R are consistent with a ˜ 5.7 day rotation period due to spots, as observed before between 1960ies and 2000. Also, for the first time, we present evidence for a long-term 9 or 17.5 year cycle in photometric data (V band) of such a young star, a cycle similar to that to of the Sun and other active stars. Based on observations obtained with telescopes of the University Observatory Jena, which is operated by the Astrophysical Institute of the Friedrich-Schiller-University; a telescope of the University Observatory Munich on Mount Wendelstein, the 0.9m ESO-Dutch telescope on La Silla, Chile, and with the All Sky Automated Survey (ASAS) project (www.astrouw.edu.pl/asas).

  8. Envenimation mortelle par morsure de serpent chez une femme enceinte

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    Abdelkarim Shimi

    2011-02-01

    Full Text Available En Afrique, la prise en charge des envenimations vipérines demeure un problème majeur de santé publique avec un taux de mortalité qui reste élevé.La survenue d’une envenimation vipérine au cours de la grossesse est un événement rare, et grave du fait des conséquences maternelles et fatales qui en découlent. Les auteurs rapportent un cas de morsure de serpent au niveau de la face chez une femme enceinte, dont l’évolution a été marquée par l’installation d’un œdème cervico-facial nécessitant une trachéotomie en urgence, et une mort fatale in utero avec troubles de l’hémostase responsable du décès maternel dans un tableau de choc hémorragique.

  9. Le Web pour enseigner par projets et favoriser la collaboration

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    Sylvie Ratté

    2004-01-01

    Full Text Available L’utilisation du Web présentée dans cet article vient appuyer une démarche pédagogique fondée sur le partage des savoirs. Dans le contexte d’une simulation industrielle, les étudiants d’un cours de programmation avancée doivent apprendre à partager leur expertise, à communiquer clairement leurs questions, à décrire des problèmes et à faire face à des retournements inattendus. Le Web devient un espace de collaboration pour diffuser les requêtes d’un client fictif, les directives, les travaux préliminaires et les ententes. Les résultats obtenus tendent à suggérer que cette approche globale force l’étudiant à mieux planifier, l’invite à mieux formuler et décrire les problèmes, augmente le niveau de collaboration et facilite la production d’analogies. Un système facilitant la logistique entourant la revue par les pairs (récupération des travaux, construction d’une grille d’évaluation et diffusion des résultats est également présenté.

  10. Monitoreo de parásitos en efluentes domiciliarios

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    Semenas Liliana

    1999-01-01

    Full Text Available OBJETIVO: Evaluar la presencia de parásitos en efluentes semisólidos y líquidos en distintas localidades de la Patagonia argentina considerando que ésta es una de las restricciones para su uso. MÉTODOS: Las muestras tomadas en 4 Plantas de Tratamiento de Efluentes Domiciliarios se analizaron siguiendo las normativas de Environmental Protection Agency, Organización Mundial de la Salud, Standard Methods for Examination of Water and Wastewater y de algunas clasificaciones. RESULTADOS: Solamente 2 de las 6 muestras de semisólidos analizadas tenían huevos de Ascaris lumbricoides no viables. De las 10 muestras líquidas, solamente 2 no tenían huevos mientras las restantes tenían patógenos de las categorías I (Giardia sp., Hymenolepis diminuta y Enterobius vermicularis y III (Ascaris lumbricoides, Ancylostoma duodenale y Trichuris trichiura. CONCLUSIONES: Todas las muestras de semisólidos analizadas fueron aptas para su uso como fertilizantes porque no se registró en ninguna de ellas la presencia de huevos viables de Ascaris lumbricoides y solamente 6 de las muestras líquidas fueron aptas para riego por carecer de huevos o por ser su concentración igual o inferior a 1 huevo por litro.

  11. Nicotine modulates GABAergic transmission to dopaminergic neurons in substantia nigra pars compacta

    Institute of Scientific and Technical Information of China (English)

    Cheng XIAO; Ke-chun YANG; Chun-yi ZHOU; Guo-zhang JIN; Jie WU; Jiang-hong YE

    2009-01-01

    Aim: Dopaminergic neurons in the substantia nigra pars compacta (SNc) play important roles in motor control and drug addiction. As the major afferent, GABAergic innervation controls the activity of SNc dopaminergic neurons. Although it is clear that nicotine modulates SNc dopaminergic neurons by activating subtypes of somatodendritic nicotinic acetylcholine receptors (nAChRs), the detailed mechanisms of this activation remain to be addressed.Methods: In the current study, we recorded GABAA receptor-mediated spontaneous inhibitory postsynaptic currents (sIP-SCs) from dissociated SNc dopaminergic neurons that were obtained using an enzyme-free procedure. These neurons preserved some functional terminals after isolation, including those that release GABA.Results: We found that both extra- and intra-cellular calcium modulates sIPSCs in these neurons. Furthermore, both nicotine and endogenous acetylcholine enhance the frequency of sIPSCs. Moreover, endogenous acetylcholine tonically facilitates sIPSC frequency, primarily by activating the a4B2* nAChRs on the GABAergic terminals.Conclusion: Nicotine facilitates GABA release onto SNc dopaminergic neurons mainly via the activation of presynaptic a4B2* nAChRs.

  12. Modulation of synaptic GABAA receptor function by zolpidem in substantia nigra pars reticulata

    Institute of Scientific and Technical Information of China (English)

    Li-li ZHANG; Lei CHEN; Yan XUE; Wing-ho YUNG

    2008-01-01

    Aim: The substantia nigra pars reticulata (SNr) constitutes one of the output centers of the basal ganglia, and its abnormal activity is believed to contribute to some basal ganglia motor disorders. Different lines of evidence revealed a major contribution of GABAA receptor-mediated synaptic inhibition in controlling the activity of SNr. The benzodiazepine binding site within the GABAA receptor is a modulation site of significant clinical interest. A high density of benzodiazepine binding sites has been reported in the rat SNr. In the present study, we investi-gate the effects of activating benzodiazepine binding sites in the SNr. Methods: Whole-cell patch-clamp recordings and motor behavior were applied. Results: Superfusion of zolpidem, a benzodiazepine binding agonist, at 100 nmol/L signifi-cantly prolonged the decay time of GABAA receptor-mediated postsynaptic currents. The prolongation on decay time induced by zolpidem was sensitive to the benzodiazepine antagonist flumazenil, confirming the specificity on the ben-zodiazepine site. Zolpidem at 1 μmol/L exerted a stronger prolongation on the decay time. A further experiment was performed on behaving rats. A unilateral microinjection of zolpidem into the rat SNr caused a robust contralateral rotation, which was significantly different from that of control animals receiving the vehicle injection. Conclusion: The present in vitro and in vivo findings that zolpidem significantly potentiated GABA currents and thus inhibited the activity of the SNr provide a rationale for further investigations into its potential in the treatment of basal ganglia disorders.

  13. MMP-1/PAR-1 signal transduction axis and its prognostic impact in esophageal squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Hong-hua Peng

    2012-01-01

    Full Text Available The matrix metalloprotease-1 (MMP-1/protease-activated receptor-1 (PAR-1 signal transduction axis plays an important role in tumorigenesis. To explore the expression and prognostic value of MMP-1 and PAR-1 in esophageal squamous cell carcinoma (ESCC, we evaluated the expression of two proteins in resected specimens from 85 patients with ESCC by immunohistochemistry. Sixty-two (72.9% and 58 (68.2% tumors were MMP-1- and PAR-1-positive, respectively, while no significant staining was observed in normal esophageal squamous epithelium. MMP-1 and PAR-1 overexpression was significantly associated with tumor node metastasis (TNM stage and regional lymph node involvement. Patients with MMP-1- and PAR-1-positive tumors, respectively, had poorer disease-free survival (DFS than those with negative ESCC (P = 0.002 and 0.003, respectively. Univariate analysis showed a significant relationship between TNM stage [hazard ratio (HR = 2.836, 95% confidence interval (CI = 1.866-4.308], regional lymph node involvement (HR = 2.955, 95%CI = 1.713-5.068, MMP-1 expression (HR = 2.669, 95%CI = 1.229-6.127, and PAR-1 expression (HR = 1.762, 95%CI = 1.156-2.883 and DFS. Multivariate analysis including the above four parameters identified TNM stage (HR = 2.035, 95%CI = 1.167-3.681, MMP-1 expression (HR = 2.109, 95%CI = 1.293-3.279, and PAR-1 expression (HR = 1.967, 95%CI = 1.256-2.881 as independent and significant prognostic factors for DFS. Our data suggest for the first time that MMP-1 and PAR-1 were both overexpressed in ESCC and are novel predictors of poor patient prognosis after curative resection. The MMP-1/PAR-1 signal transduction axis might be a new therapeutic target for future therapies tailored against ESCC.

  14. MMP-1/PAR-1 signal transduction axis and its prognostic impact in esophageal squamous cell carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Hong-hua; Zhang, Xi; Cao, Pei-guo [Department of Oncology, the Third Xiangya Hospital, Central South University, Changsha, Hunan Province (China)

    2011-11-18

    The matrix metalloprotease-1 (MMP-1)/protease-activated receptor-1 (PAR-1) signal transduction axis plays an important role in tumorigenesis. To explore the expression and prognostic value of MMP-1 and PAR-1 in esophageal squamous cell carcinoma (ESCC), we evaluated the expression of two proteins in resected specimens from 85 patients with ESCC by immunohistochemistry. Sixty-two (72.9%) and 58 (68.2%) tumors were MMP-1- and PAR-1-positive, respectively, while no significant staining was observed in normal esophageal squamous epithelium. MMP-1 and PAR-1 overexpression was significantly associated with tumor node metastasis (TNM) stage and regional lymph node involvement. Patients with MMP-1- and PAR-1-positive tumors, respectively, had poorer disease-free survival (DFS) than those with negative ESCC (P = 0.002 and 0.003, respectively). Univariate analysis showed a significant relationship between TNM stage [hazard ratio (HR) = 2.836, 95% confidence interval (CI) = 1.866-4.308], regional lymph node involvement (HR = 2.955, 95%CI = 1.713-5.068), MMP-1 expression (HR = 2.669, 95%CI = 1.229-6.127), and PAR-1 expression (HR = 1.762, 95%CI = 1.156-2.883) and DFS. Multivariate analysis including the above four parameters identified TNM stage (HR = 2.035, 95%CI = 1.167-3.681), MMP-1 expression (HR = 2.109, 95%CI = 1.293-3.279), and PAR-1 expression (HR = 1.967, 95%CI = 1.256-2.881) as independent and significant prognostic factors for DFS. Our data suggest for the first time that MMP-1 and PAR-1 were both overexpressed in ESCC and are novel predictors of poor patient prognosis after curative resection. The MMP-1/PAR-1 signal transduction axis might be a new therapeutic target for future therapies tailored against ESCC.

  15. KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation

    Science.gov (United States)

    Jha, Hem C.; Sun, Zhiguo; Upadhyay, Santosh K.; El-Naccache, Darine W.; Singh, Rajnish K.; Sahu, Sushil K.; Robertson, Erle S.

    2016-01-01

    Studies have suggested that Epithelial–Mesenchymal Transition (EMT) and transformation is an important step in progression to cancer. Par3 (partitioning-defective protein) is a crucial factor in regulating epithelial cell polarity. However, the mechanism by which the latency associated nuclear antigen (LANA) encoded by Kaposi's Sarcoma associated herpesvirus (KSHV) regulates Par3 and EMTs markers (Epithelial-Mesenchymal Transition) during viral-mediated B-cell oncogenesis has not been fully explored. Moreover, several studies have demonstrated a crucial role for EMT markers during B-cell malignancies. In this study, we demonstrate that Par3 is significantly up-regulated in KSHV-infected primary B-cells. Further, Par3 interacted with LANA in KSHV positive and LANA expressing cells which led to translocation of Par3 from the cell periphery to a predominantly nuclear signal. Par3 knockdown led to reduced cell proliferation and increased apoptotic induction. Levels of SNAIL was elevated, and E-cadherin was reduced in the presence of LANA or Par3. Interestingly, KSHV infection in primary B-cells led to enhancement of SNAIL and down-regulation of E-cadherin in a temporal manner. Importantly, knockdown of SNAIL, a major EMT regulator, in KSHV cells resulted in reduced expression of LANA, Par3, and enhanced E-cadherin. Also, SNAIL bound to the promoter region of p21 and can regulate its activity. Further a SNAIL inhibitor diminished NF-kB signaling through upregulation of Caspase3 in KSHV positive cells in vitro. This was also supported by upregulation of SNAIL and Par3 in BC-3 transplanted NOD-SCID mice which has potential as a therapeutic target for KSHV-associated B-cell lymphomas. PMID:27463802

  16. KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation.

    Directory of Open Access Journals (Sweden)

    Hem C Jha

    2016-07-01

    Full Text Available Studies have suggested that Epithelial-Mesenchymal Transition (EMT and transformation is an important step in progression to cancer. Par3 (partitioning-defective protein is a crucial factor in regulating epithelial cell polarity. However, the mechanism by which the latency associated nuclear antigen (LANA encoded by Kaposi's Sarcoma associated herpesvirus (KSHV regulates Par3 and EMTs markers (Epithelial-Mesenchymal Transition during viral-mediated B-cell oncogenesis has not been fully explored. Moreover, several studies have demonstrated a crucial role for EMT markers during B-cell malignancies. In this study, we demonstrate that Par3 is significantly up-regulated in KSHV-infected primary B-cells. Further, Par3 interacted with LANA in KSHV positive and LANA expressing cells which led to translocation of Par3 from the cell periphery to a predominantly nuclear signal. Par3 knockdown led to reduced cell proliferation and increased apoptotic induction. Levels of SNAIL was elevated, and E-cadherin was reduced in the presence of LANA or Par3. Interestingly, KSHV infection in primary B-cells led to enhancement of SNAIL and down-regulation of E-cadherin in a temporal manner. Importantly, knockdown of SNAIL, a major EMT regulator, in KSHV cells resulted in reduced expression of LANA, Par3, and enhanced E-cadherin. Also, SNAIL bound to the promoter region of p21 and can regulate its activity. Further a SNAIL inhibitor diminished NF-kB signaling through upregulation of Caspase3 in KSHV positive cells in vitro. This was also supported by upregulation of SNAIL and Par3 in BC-3 transplanted NOD-SCID mice which has potential as a therapeutic target for KSHV-associated B-cell lymphomas.

  17. Identification of uPAR-positive chemoresistant cells in small cell lung cancer.

    Directory of Open Access Journals (Sweden)

    Margarita Gutova

    Full Text Available BACKGROUND: The urokinase plasminogen activator (uPA and its receptor (uPAR/CD87 are major regulators of extracellular matrix degradation and are involved in cell migration and invasion under physiological and pathological conditions. The uPA/uPAR system has been of great interest in cancer research because it is involved in the development of most invasive cancer phenotypes and is a strong predictor of poor patient survival. However, little is known about the role of uPA/uPAR in small cell lung cancer (SCLC, the most aggressive type of lung cancer. We therefore determined whether uPA and uPAR are involved in generation of drug resistant SCLC cell phenotype. METHODS AND FINDINGS: We screened six human SCLC cell lines for surface markers for putative stem and cancer cells. We used fluorescence-activated cell sorting (FACS, fluorescence microscopy and clonogenic assays to demonstrate uPAR expression in a subpopulation of cells derived from primary and metastatic SCLC cell lines. Cytotoxic assays were used to determine the sensitivity of uPAR-positive and uPAR-negative cells to chemotherapeutic agents. The uPAR-positive cells in all SCLC lines demonstrated multi-drug resistance, high clonogenic activity and co-expression of CD44 and MDR1, putative cancer stem cell markers. CONCLUSIONS: These data suggest that uPAR-positive cells may define a functionally important population of cancer cells in SCLC, which are resistant to traditional chemotherapies, and could serve as critical targets for more effective therapeutic interventions in SCLC.

  18. The diagnostic value of soluble urokinase plasminogen activator receptor (suPAR) compared to C-reactive protein (CRP) and procalcitonin (PCT) in children with systemic inflammatory response syndrome (SIRS).

    Science.gov (United States)

    Şirinoğlu, Melis; Soysal, Ahmet; Karaaslan, Ayşe; Kepenekli Kadayifci, Eda; Yalındağ-Öztürk, Nilüfer; Cinel, İsmail; Yaman, Ali; Haklar, Goncagül; Şirikci, Önder; Turan, Serap; Altınkanat Gelmez, Gülşen; Söyletir, Güner; Bakır, Mustafa

    2017-01-01

    The aim of the present study was to determine the diagnostic and prognostic values of suPAR and to compare them to CRP and PCT in pediatric patients with systemic inflammatory response syndrome (SIRS). A prospective case-control study was performed.The study was performed in a tertiary university hospital which has a 649-bed capacity. Patients included 27 children with SIRS and 27 control subjects without any infection or immunosuppressive condition. Blood samples were obtained on the day of admission and on the 4-7th days of the hospital stay. The median (min-max) serum levels of suPAR obtained on the first day of the admission were 10.06 (2.7-57.46) and 2.22 (1.08-5.13) ng/Ml for the SIRS group and control group, respectively. The median serum levels of suPAR in the SIRS group was significantly higher than that in the control group (p SIRS group (p SIRS group, the area under the receiver operating characteristics curve (AUC(ROC)) for suPAR revealed an optimum cut-off value, sensitivity, specificity, NPV and PPV of 0.978, 3.8 ng/mL, 96%, 96%, 96%, and 96%, respectively. We conclude that suPAR does have diagnostic value in children with SIRS. Additionally, persistent high serum suPAR level predicts mortality in SIRS in children. Copyright © 2016 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  19. Provenance of the oil in par-fried French fries after finish frying.

    Science.gov (United States)

    Al-Khusaibi, Mohammed; Gordon, Michael H; Lovegrove, Julie A; Niranjan, Keshavan

    2012-01-01

    Frozen par-fried French fries are finish-fried either by using the same type of oil used for par frying, or a different type. The nutritive quality of the final oil contained in the product depends on the relative amounts and the fatty acid (FA) composition of the oils used for par frying and finish frying. With the aim of understanding the provenance of the oil in the final product, par-fried French fries-either purchased ready or prepared in the laboratory-were finish fried in oils different from the ones used for par frying. The moisture content, oil content, and FA compositions of the par-fried and finish-fried products were experimentally determined, and the relative amounts of each of the oils present in the final product were calculated using the FAs as markers and undertaking a mass balance on each component FA. The results demonstrate that 89% to 93% of the total oil in the final product originates from the finish-frying step. The study also shows that a significant proportion of the oil absorbed during par frying is expelled from the product during finish frying. Further, the expulsion of par-frying oil was found to occur in the early stages of the finish-frying step. Experiments involving different combinations of par-frying and finish-frying oils showed that the relative proportions of the 2 oils did not depend on the individual fatty acid profiles. This study concludes that any positive health benefits of using an oil having a favorable FA profile for par frying, can potentially be lost, if the oil used for finish frying has a less favorable composition. This paper estimates the relative amounts of oil in French fries that have been fried in 2 stages-a par-frying step and a finish-frying step-which is commonly practiced in food service establishments as well as homes. The 2 key conclusions are: (1) nearly 90% of the oil content of the final product is the one used for finish frying; that is, a processor may use very good oil for par frying but if the

  20. Classification structurée pour l'apprentissage par renforcement inverse

    OpenAIRE

    Klein, Edouard; Piot, Bilal; Geist, Matthieu; Pietquin, Olivier

    2012-01-01

    http://cap2012.loria.fr/pub/Papers/13.pdf; National audience; Cette contribution traite du problème de l'apprentissage par imitation par le biais de l'apprentissage par renforcement inverse (ARI). Dans ce contexte, un expert accomplit une tâche qu'un agent artificiel doit essayer de reproduire. L'ARI part du postulat que l'expert optimise avec succès une fonction de récompense ; le problème consiste à deviner cette fonction à partir de traces du comportement de l'expert. Les algorithmes d'ARI...

  1. Características morfológicas da pars de C2 de humanos

    OpenAIRE

    CARAZZO, Charles André; Guirado,Vinícius Monteiro de Paula; Meluzzi,Alexandre; Edson Aparecido LIBERTI; Dias,Mario Rubem Pena; Fontes,Ricardo Bragança de Vasconcelos; Taricco,Mario Augusto

    2009-01-01

    OBJETIVO: avaliar a morfometria da pars da segunda vértebra cervical, verificar as variações existentes e predizer sua segurança para a colocação de parafusos por meio de uma abordagem cervical posterior. MÉTODOS: 58 vértebras foram obtidas para estudo anatômico (116 pars de C2) e foram medidas a altura e largura da pars, além do comprimento eixo-pedículo, largura e comprimento do pedículo, angulação sagital e medial. Todas as medidas foram separadas quanto aos seus lados direito e esquerdo; ...

  2. Pars plana cicatrization of sewn-in posterior chamber intraocular lens haptics.

    Science.gov (United States)

    McDermott, M L; Puklin, J E

    1997-03-01

    The authors describe the unexpected finding of cicatrization of posterior chamber lens haptics to the pars plana. This was found during removal of a secondary, transscleral sewn-in pseudophakos during a retinal reattachment procedure. Ophthalmic history and intraoperative photography revealed pars plana cicatrization of haptics. Despite removal of trans-scleral prolene fixation sutures and application of gentle traction, the posterior chamber lens haptics remained firmly adherent to pars plana retina. The haptics were amputated to prevent significant chorioretinal damage. The conventional belief that all sewn-in posterior chamber intraocular lenses cause little or no scarring response around the haptics may be unfounded.

  3. Influencia de los parámetros de proceso, en el comportamiento del parámetro vibración absoluta en turbinas de vapor.

    Directory of Open Access Journals (Sweden)

    F. de la Torre Silva

    2008-01-01

    Full Text Available Este trabajo muestra el estudio realizado en turbinas de vapor de pequeña capacidad de generación de las centrales termoeléctricas cubanas, relacionadas con la influencia estadística entre parámetros de proceso de la turbina, respecto al parámetro vibración absoluta, medidos en las chumaceras de la turbina. Se utilizan las bases de datos de los sistemas de monitoreado on-line de la turbina. Se exponen las relaciones existentes entre los principales parámetros seleccionados para este estudio.This work show the study in small steam turbine capacity of generation in cubans thermoelectric power station to relative with statistical influence between steam process parameters regarding absolute vibration parameter, in the steam housing measurements. Themselves use database as steam on-line monitoring systems. Expose the existing relation between the principal selection parameters for this study.

  4. Parámetros hematológicos en pacientes opiaceodependientes Haemotologic parameters in opiate addicts

    Directory of Open Access Journals (Sweden)

    C. del M. Verde Méndez

    2003-12-01

    Full Text Available Se determinaron los niveles de hemoglobina, hematocrito, hematíes, recuento de leucocitos, índices relacionados con la serie roja (VCM, HCM, CHCM, IDE, VPM, velocidad de sedimentación, hierro, parámetros relacionados con la captación y transporte de hierro (transferrina, ferritina, capacidad de fijación de hierro, índice de saturación de transferrina y plaquetas en muestras sanguíneas de opiaceodependientes y se compararon los resultados con los obtenidos en un grupo control. En general, para ambos sexos los niveles de hemoglobina, hematocrito, hierro y plaquetas fueron similares en el grupo control y en los opiaceodependientes. Las mujeres opiaceodependientes presentaron un recuento de hematíes y leucocitos superior e inferior respectivamente, que los correspondientes valores del grupo control. Se observó que en el total de pacientes opiaceodependientes, así como en los subgrupos de mujeres y hombres, los índices relacionados con la serie roja fueron superiores con respecto al grupo control. Los niveles de ferritina en opiaceodependientes varones fueron superiores a los encontrados en los controles, mientras que en las mujeres ocurrió lo contrario. Se observó que los hombres y mujeres opiaceodependientes incluidos en el programa de mantenimiento con metadona (PMM presentaron valores de hemoglobina, hematocrito, hematíes y recuento de leucocitos similares a los no incluidos, exceptuando los heroinómanos varones incluidos en el PMM que mostraron valores inferiores de hematocrito.Levels of haemoglobin, haematocrit, number of red cells and leukocytes, haematological index (MCV, MCH, MCHC, DEI, MPV, sedimentation rate, iron, parameter related to accumulation and transport of iron (transferrin, ferritin, iron binding capability, transferring saturation index and platelets were determined in blood samples of opiate addicts and they were compared with these results obtained in a control group. For both sexes, the levels of haemoglobin

  5. Urine suPAR levels compared with plasma suPAR levels as predictors of post-consultation mortality risk among individuals assumed to be TB-negative: a prospective cohort study

    DEFF Research Database (Denmark)

    Rabna, Paulo; Andersen, Andreas; Wejse, Christian;

    2010-01-01

    -suPAR), thereby exploring the possibility of replacing the blood sample with an easy obtainable urine sample. We enrolled 1,007 adults, older than 15 years of age, with a negative TB diagnosis between April 2004 and December 2006. Levels of U-suPAR and P-suPAR were available in 863 individuals. U......-suPAR was measured using a commercial ELISA (suPARnostic®). We found that U-suPAR carried significant prognostic information on mortality for HIV-infected subjects with an area under the ROC curve of 0.75. For HIV-negative individuals, little or no prognostic effect was observed. However, in both HIV positives...... and negatives, the predictive effect of U-suPAR was found to be inferior to that of P-suPAR....

  6. Diffusion-controlled intergranular penetration and embrittlement of copper by liquid bismuth between 300 and 600 Celsius degrees; Penetration intergranulaire fragilisante du cuivre par le bismuth liquide: identification de la cinetique et du mecanisme de type diffusionnel entre 300 et 600 deg

    Energy Technology Data Exchange (ETDEWEB)

    Laporte, V

    2005-02-15

    Hybrid reactors are a new concept for energy production and nuclear waste treatment. Among other requirements, structural materials have to withstand liquid metal embrittlement. This thesis aimed therefore to identify the controlling mechanism for the intergranular embrittlement of copper in contact with liquid bismuth. Scanning electron microscopy, Auger electron spectroscopy, X-ray photoelectron spectroscopy and Rutherford backscattering spectroscopy have been used to analyze fracture surfaces of both copper polycrystals and a copper bicrystal (symmetric tilt boundary 50 degrees <100>). These analyses reveal both parabolic intergranular penetration kinetics and a maximal intergranular bismuth concentration that is less than two monolayers equivalent. These two results allow us to identify grain boundary diffusion as the controlling mechanism for the intergranular penetration of copper by liquid bismuth between 300 and 600 Celsius degrees, showing the absence of perfect grain boundary wetting. (author)

  7. Air breath control radiotherapy in severe insufficiency respiratory patients with N.S.C.L.: application for deformable registration method in thoracic radiotherapy; Radiotherapie avec blocage respiratoire pour les grands insuffisants respiratoires atteints d'un carcinome pulmonaire non a petites cellules (Protocole RESPI 2000): application a la modelisation des deformations d'organes par recalage deformable

    Energy Technology Data Exchange (ETDEWEB)

    Sarrut, D.; Pommier, P.; Carrie, C. [Centre Leon-Berard, Dept. de Radiotherapie, CREATIS, Unite CNRS 5515, Inserm 630, 69 - Lyon (France); Perol, D. [Centre Leon-Berard, Dept. de Biostatistique, 69 - Lyon (France)

    2006-11-15

    Purpose. Using deformable registration methods from a phase two clinical study of air breath control during radiotherapy in patients suffering from severe respiratory insufficiency and non-small cell lung carcinoma. Patients and methods, Between April 2002 and November 2005, 22 patients with severe respiratory insufficiency were treated with curative intent by conformal therapy combined with active breathing control. Results. After a mean of follow-up of 22 months, the local control rate is 28% and the method is feasible despite the severe respiratory insufficiency. However the overall survival is still poor due to metastatic widespread. For the second part of the study, the clinical protocol was also used for two studies using deformable registration methods. In the first study, a deformable registration method has been developed in order to register several breath-hold 3D CT of the same patient acquired at several days of interval. It allowed quantifying the inter-fraction breath-hold reproducibility by analysing the resulting displacement field. For 6 patients, the breath-hold was effective, while for 2 patients, motion greater than 10 mm were detected. The second study aimed to simulate 4D images from 3D breath-hold images. Developing an ad-hoc methodology based on the interpolation of 3D dense deformation fields performed it. The approach has been validated with expert selected landmarks, with accuracy lower than 3 mm. Conclusion. ABC is feasible, even in case of severe insufficiency respiratory syndrome but metastatic widespread disease is still a major challenge even with an acceptable local control rate without serious side effects: regarding the deformable registration method. Such artificial 4D images could allow decreasing the dose need to acquire a full 4D image, to simulate irregular breathing pattern and to be used for 4D dosimetry planning. (author)

  8. La relecture du romancero par les vihuelistes de la Renaissance

    Directory of Open Access Journals (Sweden)

    Pascal Martel

    1999-01-01

    Full Text Available Les romances espagnols de la Reconquête relataient sur le mode épique des faits passés sur une mélodie récurrente qui servait surtout d’outil mnémotechnique pour réciter de longues narrations. Lorsque Diego Pisador reprend un romance, il n’en prend que quelques vers : du coup, le rapport entre les mots et les notes se fait plus étroit. D’un poème épique, il élabore une amplification lyrique, une véritable composition musicale, opère un véritable changement d’esthétique : le passage de la longue narration sur un thème musical bref et récurrent qui caractérisait le romance médiéval de la Reconquista, à une forme musico-littéraire plus brève mais plus élaborée, propre à introduire la vibration lyrique dans la matière épique. Familier de la Cour, il entend ainsi servir les desseins politiques de Philippe Il à qui il dédie son œuvre : le compositeur vihueliste qu’était Pisador en effet participe à l’effort de l’intégration du Maure poursuivi par la politique de la Maison d’Autriche. Mais les noces n’eurent pas lieu et les Maures furent au contraire expulsés en 1609. L’analyse de cette chanson nous donne l’occasion de découvrir les moyens employés par le musicien pour se livrer à une véritable amplification lyrique d’un simple récit par la musique. La chanson savante n’est pas non plus une composition dans laquelle la mélodie traduirait simplement en musique le texte poétique de façon tout à fait parallèle : la musique n’est pas qu’une répétition, sous une autre forme, de la parole, ou bien l’image sonore d’un texte. Musique et poésie constituent deux moyens autonomes d’expression mais qui, dans une chanson, concourent à un même but : ces deux moyens d’expression tantôt se rejoignant, tantôt s’émancipant l’un de l’autre, de sorte que le propre de la chanson réside justement dans cette tension constante entre ses deux composantes. La mise en musique

  9. PAR-Aware Large-Scale Multi-User MIMO-OFDM Downlink

    CERN Document Server

    Studer, Christoph

    2012-01-01

    We investigate an orthogonal frequency-division multiplexing (OFDM)-based downlink transmission scheme for large-scale multi-user (MU) multiple-input multiple-output (MIMO) wireless systems. The use of OFDM causes a high peak-to-average (power) ratio (PAR), which necessitates expensive and power-inefficient radio-frequency (RF) components at the base station. In this paper, we present a novel downlink transmission scheme, which exploits the massive degrees-of-freedom available in large-scale MU-MIMO-OFDM systems to achieve low PAR. Specifically, we propose to jointly perform MU precoding, OFDM modulation, and PAR reduction by solving a convex optimization problem. We develop a corresponding fast iterative truncation algorithm (FITRA) and show numerical results to demonstrate tremendous PAR-reduction capabilities. The significantly reduced linearity requirements eventually enable the use of low-cost RF components for the large-scale MU-MIMO-OFDM downlink.

  10. Inmunidad frente a infecciones: bacterias, virus, hongos y parásitos

    OpenAIRE

    Sen Fernández, María Luz de la; Sempere Ortells, José Miguel; Marco de la Calle, Francisco Manuel; Vázquez Araujo, Begoña

    2012-01-01

    Respuesta inmune frente a la infección: Bacterias extracelulares, bacterias intracelulares, hongos, virus, parásitos. Inmunidad innata, inmunidad adaptativa, mecanismos de evasión, consecuencias perjudiciales de la respuesta inmune.

  11. Soluble urokinase plasminogen activator receptor (suPAR) in acute care

    DEFF Research Database (Denmark)

    Rasmussen, Line Jee Hartmann; Ladelund, Steen; Haupt, Thomas Huneck

    2016-01-01

    with age, admission time, admission to intensive care unit and Charlson score. CONCLUSIONS: In this large unselected population of acute medical patients, suPAR is strongly associated with disease severity, readmission and mortality after adjusting for all other risk factors, indicating that suPAR adds....... METHODS: This registry-based retrospective cohort study included 4343 consecutively admitted patients from the Acute Medical Unit at a large Danish university hospital. Time to readmission and death were analysed by multiple Cox regression. Results were reported as HRs for 30-day and 90-day follow......OBJECTIVE: Soluble urokinase plasminogen activator receptor (suPAR) is an inflammatory biomarker associated with presence and progression of disease and with increased risk of mortality. We aimed to evaluate the unspecific biomarker suPAR as a prognostic marker in patients admitted to acute care...

  12. Urokinase receptor (uPAR) ligand based recombinant toxins for human cancer therapy.

    Science.gov (United States)

    de Virgilio, Maddalena; Silvestris, Franco

    2011-01-01

    The urokinase receptor (uPAR) exerts essential functions in the pathophysiology of cancers and therefore constitutes an important drug target. In order to generate efficient drugs against uPAR, a new approach includes chimeric proteins associating one molecular address to specifically target uPAR and one bacterial or plant toxin that will eventually kill the tumoural cell. Using this frame, several recombinant toxins have been designed namely DTAT, DTAT13, EGFATFKDEL 7 mut, and ATF-SAP. As molecular address, all of these fusion proteins use the amino-terminal fragment of urokinase that binds with high affinity to uPAR through its growth factor domain (GFD). The various toxin moieties were derived from either diphtheria toxin, Pseudomonas exotoxin A (PE38), or saporin. In this review, we describe the rational, design, production and therapeutic anti-cancer potential of these chimeric toxins.

  13. Efectos biológicos de la terapia del par biomagnético

    OpenAIRE

    2016-01-01

    Esta tesis está centrada a comprobar el efecto de los imanes según la técnica del Par Biomagnético sobre el cuerpo, midiendo el efecto que tienen sobre la excitabilidad neuromuscular. El Par biomagnético es un método creado por el Dr Goiz Durán basado en la impactación de imanes estáticos de 1000 gaus sobre el cuerpo. Según el Dr Goiz el Par Biomagnético tiene la capacidad de estudiar, detectar, clasificar, medir y corregir las alteraciones funcionales del pH de los órganos vivos. El Par biom...

  14. ParFit: A Python-Based Object-Oriented Program for Fitting Molecular Mechanics Parameters to ab Initio Data.

    Science.gov (United States)

    Zahariev, Federico; De Silva, Nuwan; Gordon, Mark S; Windus, Theresa L; Dick-Perez, Marilu

    2017-03-27

    A newly created object-oriented program for automating the process of fitting molecular-mechanics parameters to ab initio data, termed ParFit, is presented. ParFit uses a hybrid of deterministic and stochastic genetic algorithms. ParFit can simultaneously handle several molecular-mechanics parameters in multiple molecules and can also apply symmetric and antisymmetric constraints on the optimized parameters. The simultaneous handling of several molecules enhances the transferability of the fitted parameters. ParFit is written in Python, uses a rich set of standard and nonstandard Python libraries, and can be run in parallel on multicore computer systems. As an example, a series of phosphine oxides, important for metal extraction chemistry, are parametrized using ParFit. ParFit is in an open source program available for free on GitHub ( https://github.com/fzahari/ParFit ).

  15. Características morfológicas da pars de C2 de humanos Características morfológicas de la pars de C2 de humanos Morphologic characteristics of C2 humans pars

    Directory of Open Access Journals (Sweden)

    Charles André Carazzo

    2009-09-01

    Full Text Available OBJETIVO: avaliar a morfometria da pars da segunda vértebra cervical, verificar as variações existentes e predizer sua segurança para a colocação de parafusos por meio de uma abordagem cervical posterior. MÉTODOS: 58 vértebras foram obtidas para estudo anatômico (116 pars de C2 e foram medidas a altura e largura da pars, além do comprimento eixo-pedículo, largura e comprimento do pedículo, angulação sagital e medial. Todas as medidas foram separadas quanto aos seus lados direito e esquerdo; foi realizado o cálculo da média, variação, desvio padrão e a médica foi comparada com os estudos prévios. RESULTADOS: a largura média da pars de C2 foi de 9,5 mm; a altura, 9,5 mm; não houve diferença significativa entre os lados esquerdo e direito. O comprimento médio do pedículo foi de 10,5 mm; a medida média encontrada para o comprimento eixo-pedículo foi de 24,9 mm; a largura do pedículo foi de 2,8 mm. A angulação sagital encontrada foi de 19,6º e a horizontal foi de 28,5º. CONCLUSÕES: este estudo demonstra a viabilidade para a colocação de parafuso na pars de C2 em uma população caucasiana, destacando-se, porém, a grande variabilidade anatômica encontrada nas referidas medidas.OBJETIVO: evaluar la morfometría de la pars de la segunda vértebra cervical, verificar las variaciones existentes y predecir su seguridad para la colocación de los tornillos por un abordaje cervical posterior. MÉTODOS: 58 vértebras fueron obtenidas para el estudio anatómico (116 pars de C2 y fueron medidas su altura y el ancho de las pars, además de la largura eje-pedículo, ancho y largura del pedículo, ángulo sagital y medial. Todas las medidas fueron separadas según sus lados derecho e izquierdo, se realizó el cálculo de la media, variación, desvío estándar y comparados los valores con estudios previos. RESULTADOS: el ancho promedio de la pars de C2 fue de 9.5 mm, la altura de 9.5 mm, sin diferencia significativa entre los

  16. Study of a system devoted for ultrasonic non destructive testing of complex geometry pieces using smart contacts transducers; Etude d'un systeme de controle par ultrasons des pieces de geometrie conplexe a l'aide de traducteurs contacts intelligents

    Energy Technology Data Exchange (ETDEWEB)

    Chatillon, S

    2000-07-01

    This work is devoted to the enhancement of the ultrasonic non destructive testing in contact of nuclear components with complex geometry. In service inspections of such components performed with conventional probes present limited performances: variations in sensitivity, due to unmatched contact, incorrect characterization of the defect, because of the disorientations of the transducer during its displacement, and uncovered scan area when the geometry of the components disturbs the displacement of the transducer. We propose a new concept of smart transducer to improve the performances of such inspections. The radiating surface is flexible to optimize the sensitivity of the testing. Using the measure of the radiating surface distortion, performed by a specific instrumentation, phased array techniques allow the control of the transmitted beam to optimize the defect localization and characterization. Thus, this system is self-contained. We present the different steps involved to develop this system and its experimental validation. A computing model is extended to predict the field transmitted by a flexible contact transducer. This model is used to optimize the radiating surface of a jointed transducer. A delay law optimizing algorithm is developed to ensure the control of the transmitted beam. At last, a method and the associated instrumentation designed to measure the radiating surface distortion are proposed. Experimental Measures in the through-transmission mode validate the ability of this system to control the field transmitted through complex interfaces. At last, inspections in the pulse-echo mode are performed on a specimen with an irregular profile, representative of a real component inspected on site, and artificial embedded reflectors. Two control configurations are used. In the first one, the transducer is displaced along the surface, in the second one, the transducer is fixed and the region of interest is scanned using beam steering. The results show that

  17. Parâmetros genéticos e eficiência relativa de seleção para a produção de leite no dia do controle para vacas da raça Holandesa Genetic parameters and relative selection efficience for milk production on test day model for Holstein cows

    Directory of Open Access Journals (Sweden)

    Juliano Andreazza

    2008-04-01

    Full Text Available Para estudar a viabilidade da utilização das produções de leite no dia do controle (PLDC em avaliações genéticas, foram utilizados 33.775 controles mensais da primeira lactação de 4.241 vacas da raça Holandesa, filhas de 561 touros, distribuídas em 23 rebanhos no Estado do Rio Grande do Sul, no período de 1992 a 2001. Os componentes de (covariância foram obtidos pelo método da Máxima Verossimilhança Restrita, com modelo animal. Os modelos consideraram as PLDC e as produções em 305 dias (PL305, segundo os efeitos aleatórios, genético aditivo direto, de ambiente permanente e residual, e dos efeitos fixos de grupo de contemporâneos e rebanho, além das covariáveis, idade da vaca ao parto e intervalo parto primeiro controle (somente para as PLDC, com os componentes linear e quadrático. As estimativas de herdabilidade para as PLDC e as correlações genéticas destas com PL305 foram altas, sugerindo que as PLDC podem ser utilizadas em avaliações genéticas em substituição a PL305. A eficiência relativa de seleção das PLDC como critério de seleção foi superior em relação à PL305.To verify the possibility of the Test Day Model Methodology (PLCD to be used in genetic evaluations, records on 33,775 monthly test day were studied. The records were obtained from 4,241 first lactation Holstein cows, sired by 561 bulls, distributed in 23 herds in the state of Rio Grande do Sul, from 1992 to 2001. The (covariance components were estimated by Restricted Maximun Likelihood method, on animal model. The model considered PLCD and 305 days yield (PL305, as functions of the aditive genetic direct, permanent environmental, and residual random effects; the contemporary group and the herd as fixed effects and the covariables, age of cow at birth and calving-first control interval (only for PLCD, linear and quadratics effects. The high heritabilities for PLCD and the high genetic correlations estimated among PLCD and PL305 suggest that

  18. Ambroise Paré IV: The early history of artificial limbs (from robotic to prostheses).

    Science.gov (United States)

    Hernigou, Philippe

    2013-06-01

    One of the earliest written references to prosthetics is found in a book published in France in 1579. That year, French surgeon Ambroise Paré (1510-1590) published his complete works, part of which described some of the artificial limbs he fitted on his amputees. As a military surgeon, Paré had removed many a soldier's shattered arm or leg, and he eventually began designing and building artificial limbs to help the men who had been maimed.

  19. [Investigation of a measles outbreak in Pará State, Brazil, in the age of elimination of the disease].

    Science.gov (United States)

    Jesus, Hiane Santos de; Nascimento, Gilmara Lima; Rosa, Fabiano Marques; Santos, Deise Aparecida dos

    2015-10-01

    In July 27th, 2010, witnessed the late notification of a positive test result for measles IgM antibodies in Belém, Pará State, Brazil, sparking an epidemiological investigation and control and preventive measures. Two more confirmed cases were identified, both of whom were siblings of the index case, with clinical signs and symptoms and incubation period consistent with measles. We conducted a retrospective search in hospitals and laboratories for suspected cases that lived in or had visited Pará State from May 1st to August 4th, 2010, and had presented fever and exanthema accompanied by cough and/or sneezing and/or conjunctivitis. All identified cases were investigated by telephone contact and/or home visits. We reviewed 183,854 consultation forms and identified 56 (0.03%) suspected cases. We applied 2,535 doses of triple viral vaccine distributed between blockades vaccination intensifications. A household measles outbreak occurred in Belém with the detection and isolation of a viral genotype imported from Europe. Timely and sensitive epidemiological surveillance is recommended for the detection of suspected cases of measles and maintenance of high immunization coverage.

  20. ON THE PAR REDUCTION OF MB-OFDM ULTRA-WIDEBAND SIGNALS

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The Peak to Average power Ratio (PAR) of a Multi-Band Orthogonal Frequency-Division Multi-plexing (MB-OFDM) Ultra-Wide Band (UWB) signals can be substantially larger than that of single carrieror carrier-less ultra-wideband signals. In this letter, a novel PAR reduction scheme for the MB-OFDM UWBsystem based on spreading and interleaving is proposed. By spreading the coded bits over each subcarrier incorresponding band and interleaving the spread symbols across all bands, the PAR statistics of theMB-OFDM signals can be improved and the PAR is reduced obviously. In the PAR reduction scheme, there isno loss in transmission data rate or Bit Error Rate (BER) performance decreasing. Since the spreading andinterleaving operation are implemented by unitary Hadamard sequences and used for an approach to providethe robustness of the UWB system to narrowband interference, there is no additional implementation burden.Simulation results show that the investigated scheme gives the PAR reduction of 3dB compared with that ofthe original MB-OFDM signals.

  1. Examining relational empowerment for elementary school students in a yPAR program.

    Science.gov (United States)

    Langhout, Regina Day; Collins, Charles; Ellison, Erin Rose

    2014-06-01

    This paper joins relational empowerment, youth empowerment, and Bridging Multiple Worlds frameworks to examine forms of relational empowerment for children in two intermediary institutions-school and a youth participatory action research after-school program (yPAR ASP). Participants were twelve children, most of whom were Latina/o and from im/migrant families, enrolled in a yPAR ASP for 2 years. A mixed-method approach was utilized; we analyzed children's interviews, self-defined goals, and their social networks to examine their experiences of relational empowerment. We conclude that children experienced each of the five relational empowerment factors-collaborative competence, bridging social divisions, facilitating others' empowerment, mobilizing networks, and passing on a legacy-in the yPAR ASP setting, and some factors in school. These experiences, however, were more pronounced in the yPAR ASP setting. Additionally, social network analyses revealed that a small but meaningful percentage of actors bridged worlds, especially home and family, but by year 2, also school and the yPAR ASP. Finally, most helpers for school-based goals came from school, but a sizable number came from family, friends, and home worlds, and by year 2, also came from the yPAR ASP. Implications range from theoretical to methodological development, including the use of social network analysis as a tool to descriptively examine relational power in context.

  2. Hep par-1: a novel immunohistochemical marker for differentiating hepatocellular carcinoma from metastatic carcinoma.

    Science.gov (United States)

    Hanif, Razia; Mansoor, Samina

    2014-03-01

    To evaluate the diagnostic utility of Hep par-1 in differentiating hepatocellular carcinoma from metastatic carcinoma taking histopathology as a gold standard. Comparative cross-sectional study. Pathology Department, Shaukat Khanum Memorial Cancer Hospital and Research Centre, Lahore, from April 2007 to February 2008. Hep par-1 immunohistochemical stain was performed on 60 cases of liver carcinoma, 30 cases each of metastatic and hepatocellular carcinoma. Information regarding patient age, gender, sign and symptoms, radiographic findings, histological grade of tumour, and expression of Hep par-1 on hepatocellular and metastatic carcinoma were recorded on proforma sheet. Sensitivity, specificity, positive and negative predictive values, and accuracy of Hep par-1 were calculated using the formulas. Hep par-1 expression was noted in 25 out of 30 cases of hepatocellular carcinoma (83%). Out of 30 cases of metastatic carcinoma, only one case expressed staining in Hep par-1 is a reliable immunohistochemical marker for cases of hepatocellular carcinoma (HCC). It can be used along with other markers in morphologically difficult cases when differential diagnosis lies between poorly differentiated HCC and metastatic carcinoma of liver.

  3. Valoración electrofisiológica de la representación cortical mediante potenciales evocados somatosensoriales en pacientes con parálisis facial periférica

    OpenAIRE

    Rincón Roncancio, Mónica

    2011-01-01

    En un estudio de casos y controles, con un grupo de 16 pacientes con parálisis facial periférica y 16 sujetos sanos, se registraron Potenciales Evocados Somatosensoriales del trigémino (PESS) y se estudiaron las latencias de las ondas N13, P19 y N30. Se encontró un desbalance funcional en el procesamiento cortical de la información somatosensorial en los pacientes con parálisis facial que se hizo manifiesto a través de: disminución significativa de la latencia del componente N13 en el lado sa...

  4. Solution-printed organic semiconductor blends exhibiting transport properties on par with single crystals

    KAUST Repository

    Niazi, Muhammad R.

    2015-11-23

    Solution-printed organic semiconductors have emerged in recent years as promising contenders for roll-to-roll manufacturing of electronic and optoelectronic circuits. The stringent performance requirements for organic thin-film transistors (OTFTs) in terms of carrier mobility, switching speed, turn-on voltage and uniformity over large areas require performance currently achieved by organic single-crystal devices, but these suffer from scale-up challenges. Here we present a new method based on blade coating of a blend of conjugated small molecules and amorphous insulating polymers to produce OTFTs with consistently excellent performance characteristics (carrier mobility as high as 6.7 cm2 V−1 s−1, low threshold voltages of<1 V and low subthreshold swings <0.5 V dec−1). Our findings demonstrate that careful control over phase separation and crystallization can yield solution-printed polycrystalline organic semiconductor films with transport properties and other figures of merit on par with their single-crystal counterparts.

  5. Computed tomographic findings in the pituitary gland and brain of horses with pituitary pars intermedia dysfunction.

    Science.gov (United States)

    Pease, A P; Schott, H C; Howey, E B; Patterson, J S

    2011-01-01

    Pituitary pars intermedia dysfunction (PPID) is the most common endocrinologic disorder of aged horses. Pituitary glands of PPID-affected horses are larger than those of aged horses without signs of PPID, and the size difference can be detected using computed tomography (CT) imaging. Eight horses with clinical signs of PPID and supportive endocrinologic test results and 3 aged control (PPID-negative) horses. Computed tomography examination of the brain and pituitary gland was performed twice in 10 of the 11 horses, approximately 6 months apart. Six PPID-affected horses were treated with pergolide for 6 months between CT scans. The second CT scan was followed by euthanasia and pathologic examination of 6 PPID-affected horses (4 treated horses). On initial examination, pituitary glands of PPID-affected horses were larger in height (P pituitary gland length increased (P pituitary gland measurements made at the terminal CT scans and necropsy. Furthermore, pituitary gland volume calculated from the measurements was highly correlated to pituitary gland weight. Additional CT findings were bilaterally symmetrical mineralization in the thalamus and cholesterol granulomas adjacent to the lateral and fourth ventricles. CT is a useful imaging modality to determine pituitary gland size of PPID-affected horses,and CT measurements are similar to gross pathologic measurements. Copyright © 2011 by the American College of Veterinary Internal Medicine.

  6. Serological survey of Neospora caninum in dairy herds from Parauapebas, State of Pará

    Directory of Open Access Journals (Sweden)

    Clovis Laurindo Silva

    2015-02-01

    Full Text Available Neospora caninum is a coccidian protozoan that affects cattle worldwide causing economic losses. To survey the frequency of anti-N. caninum antibodies in dairy herds in the municipality of Parauapebas, Southeast of Pará, samples of 465 sera from 45 farms were subjected to indirect immunofluorescence assay (cutoff 1:100. Anti-N. caninum antibodies were found with a frequency of 13,33% with the following titer distribution: 27 (43,55% for titer 100; 14 (22,58% for 200; 16 (25,80% for 400, and 5 (8,07% for 800; no serum titers more than 800 was noted, but at least one positive animal was present in most farms. The highest frequency occurred among females, with no significant difference in frequency between the sexes (Fisher exact test = 0,59, P = 0,99; 2-year-old animals had the highest frequency, although their numbers were lesser than those aged >5 years, with no significant difference in frequency between the age groups (?2 = 2,1, P = 0,71. No significant difference was observed between the frequency and occurrence of abortion (?2 = 2,3, P = 0,13 and frequency and presence of dogs in the farms (?2 = 0,26, P = 0,60. Actions toward health monitoring are recommended to prevent the entry of new sources of N. caninum and to control its spread within herds.

  7. Apprendre et maîtriser LabVIEW par ses applications

    CERN Document Server

    Martaj, Nadia

    2014-01-01

    Cet ouvrage traite de l'apprentissage du langage LabVIEW a travers ses applications dans des domaines industriels et academiques, qui permettront a l'ingenieur, technicien ou etudiant d'apprehender rapidement et efficacement ce langage. L'ouvrage commence, dans la partie I, par traiter les differents types de donnees du langage LabVIEW (tableaux, clusters, complexes, chaines de caracteres...), leur manipulation dans des structures d'execution (boucles While, For, la structure Condition, etc.), le langage textuel MathScript, des scripts Matlab, la boite de calcul utilisant la syntaxe du langage C ainsi que les nœuds de propriete qui permettent d'obtenir ou definir la propriete d''un VI. Cette etude est menee a travers des applications d'ingenierie.La partie II est consacree a l'etude de l'outil « Conception de controle et simulation » avec lequel nous pouvons simuler des systemes analogiques ou discrets.La partie III contient differentes applications qui traitent de nombreux themes comme la regulation (diff...

  8. Establecimiento de los parámetros rutinarios de control para la irradiación de suplementos alimenticios deshidratados y de material médico descartable en la planta de irradiación multiuso del Perú

    OpenAIRE

    del Valle, Carlos; Vargas, Johnny; Linares, Marco

    2002-01-01

    Mediante la realización de pruebas de irradiación a productos simulados “Dummy”, de densidades similares a los suplementos alimenticios como la maca, uña de gato, hercampuri, entre otros y a productos de uso médico como los apósitos quirúrgicos, se ha logrado determinar el tiempo de exposición necesario para obtener la dosis mínima y máxima requerida dentro del control rutinario para su irradiación a gran escala en la Planta de Irradiación Multiuso del Perú, con el propósito de su descontamin...

  9. Direito autoral na produção publicitária: o caso das paródias e paráfrases

    Directory of Open Access Journals (Sweden)

    Raquel Marques Carriço Ferreira

    2013-11-01

    Full Text Available O presente trabalho discute os direitos autorais nas criações publicitárias que se valem das paródias e paráfrases para sua formulação. Segundo a lei que rege as normas relativas à proteção intelectual, paráfrases e paródias ficam isentas da necessidade de autorização e licença dos autores das obras referenciadas no material publicitário. Na construção do discurso publicitário, entretanto, as práticas imitativas acabam por tomar rumos que não necessariamente são identificadas como tais, e como isso, demandas jurídicas são incitadas. A proposta deste artigo é apresentar como o material publicitário construído a partir de associações de ideias, alusões, adaptações, mistura de materiais discursivos, têm sido interpretadas pelos profissionais da área legal. Casos reais são trazidos ao texto para ilustrar como a produção publicitária vem se valendo deste método criativo e como os ditames legais têm tratado a questão.

  10. Ocular trauma treated with pars plana vitrectomy: early outcome report

    Science.gov (United States)

    Mansouri, Mohammad Reza; Tabatabaei, Seyed Ali; Soleimani, Mohammad; Kiarudi, Mohammad Yaser; Molaei, Saber; Rouzbahani, Mehdi; Mireshghi, Meysam; Zaeferani, Mohsen; Ghasempour, Mehrbod

    2016-01-01

    AIM To evaluate demographic variables and visual outcomes, among patients with ocular injuries involving the posterior segment, managed with pars plana vitrectomy. METHODS The records of patients were studied retrospectively from March to September 2010, to determine the age, gender, place of occurrence of trauma, visual acuity, anatomical site, nature of injury, wound length, the presence of an afferent pupillary defect, and the timing of vitrectomy. The Ocular Trauma Score was measured. The minimum follow-up from presentation was 6mo. RESULTS Ninety patients (77 males, 13 females), with a mean age of 32.7±15.8y were included over the 6-month period. The majority of cases occurred in the workplace (47 patients), followed by home (14 patients). The mean visual acuity (logMAR) of patients significantly improved from 2.36±0.72 preoperatively to 1.50±1.14 postoperatively. Twenty-three patients had preoperative vision better than 2.0 logMAR, the postoperative visual acuity was significantly better among these patients than patients with worse than 2.0 logMAR (P7d) was not significantly different (P=0.66). Postoperative visual acuity was not significantly different between patients with injury in Zone I and II (P=0.64), but patients with injury in Zone III had significantly poorer visual acuity (P=0.02). Patients with relative afferent pupillary defect had significantly poorer postoperative visual acuity (P=0.02). Preoperative visual acuity, the difference of preoperative and postoperative visual acuity, and postoperative visual acuity were significantly different between groups with different ocular trauma scores (P<0.001). CONCLUSION Trauma is more likely to occur in men under 40y of age and in the workplace. The favorable final visual outcome is associated with the absence of afferent pupillary defect, ocular trauma score and presenting visual acuity as well as the zone of injury, and not associated with the timing of vitrectomy. PMID:27275432

  11. Etiología en parálisis cerebral.

    Directory of Open Access Journals (Sweden)

    Patricia Campos

    1996-07-01

    Full Text Available Objetivo: Determinar las causas mas frecuentes de parálisis cerebral (PC. Material y métodos: Estudio descriptivo realizado en Hospital Nacional Cayetano Heredia y el Hogar Clínica San Juan de Dios (Lima-Perú, entre noviembre 1990 y mayo de 1992. Se revisaron las historias clínicas de 102 pacientes con diagnósticos de PC y se revisó las etiologías. Resultados: En relación a edad gestacional hubieron 15 casos de prematuros (14.7%, 67(65% a término y un postmaduro, en 19 no se tuvo la edad gestacional. El peso al nacer fue adecuado en 56(54.9%, bajo en 17(16.7% y muy bajo en 7(6.9%. Se identificó la causa de PC en 73/102 casos (70%, siendo las causas más frecuentes las perinatales (59.3%, seguidas de las prenatales con 29.1% y las postnatales con 11.6%. En el grupo de causas prenatales, la mitad fueron malformaciones cerebrales congénitas entre las que destaca el quiste porencefálico, presente en la mitad de estos casos. En el grupo perinatal, la más frecuente fue asfixia (83% asociada o no a otro factor metabólico. Hubieron además en este grupo 4 casos de hipoglicemia severa y 2 con encefalopatía hiperbilirrubinémica y PC distónica. Las causas postnatal más frecuente fue la meningoencefalitis bacteriana, en menores de un año de edad. Al correlacionar etiología y momento del daño, peso al nacer y tipo de PC se observa que, en los recién nacidos PT de bajo o muy bajo peso al nacer es mas frecuente la PC de tipo dipléjica y en todos el antecedente fue asfixia perinatal. Conclusiones: La baja frecuencia de PC dipléjica y la elevada frecuencia de causas perinatales (asfixia, marca nuestra diferencia con otras series y obedece en nuestra opinión, a los escasos recursos tecnológicos y las deficiencias en la atención a las gestantes y recién nacidos de nuestro sistema nacional de salud. (Rev Med Hered 1996; 7: 114-118.

  12. N.7 notice presented for the Finances Commission, of the budget control and the economical accounts of the Nation on the law project, adopted by the National Assembly after urgency declaration, relative to the energy sector; N.7 avis presente au nom de la commission des Finances, du controle budgetaire et des comptes economiques de la Nation sur le projet de loi, adopte par l'Assemblee Nationale apres declaration d'urgence, relatif au secteur de l'energie

    Energy Technology Data Exchange (ETDEWEB)

    Marini, Ph

    2006-10-15

    This law project concerns the privatization of Gaz de France and the new control of the State on this society. It underlines the necessity of a financial independence of the Commission of the Energy regulation (CRE). (A.L.B.)

  13. Non destructive testing of industrial pieces by radiography: quantitative characterization and 3 D reconstruction by the way of a limited number of images; Controle non destructif de pieces industrielles par radiographie: caracterisation quantitative et reconstruction 3D a partir d`un nombre limite de vues

    Energy Technology Data Exchange (ETDEWEB)

    Retraint, F

    1998-12-31

    The non destructive testing of industrial pieces is evaluated on the basis of numerical radiographies.The context of the study is the online control of the fuel rods production. A direct model of a numerical radiography formation is proposed and detailed for an acquisition system consisting of a CCD video connected to a converter screen by an optical system. As this approach does not allow the determination of the measured matter thickness from the X-ray photograph, an approximate model based on realistic approximations of the industrial non destructive testing, has been developed. For the specific cases it is possible to inverse the model and to reach the quantitative information present in the x-ray photograph, in other words, the map of the X-rays measured matter thickness. It becomes then possible to access to the quantitative parameters of the possible defects present in the measured specimen, such as the surface and the bulk. To reach the 3 D information on the defects a 3 D reconstruction method, from 3 X-rays photographs, is proposed.The inverse problem is solved by the non convex energy minimization. (A.L.B.) 109 refs.

  14. Efeitos de diferentes tipos de exercício nos parâmetros do andar de idosas

    Directory of Open Access Journals (Sweden)

    Jozilma de Medeiros Gonzaga

    2011-06-01

    Full Text Available O tipo de exercício, a intensidade e a frequência são fatores importantes para produzir mudanças na velocidade de andar. O objetivo do estudo foi comparar os efeitos de diferentes tipos de exercício nos parâmetros cinemáticos do andar de idosas, considerando as características antropométricas, a capacidade funcional e o nível de atividade física. Participaram do estudo 56 idosas que foram agrupadas de acordo com o envolvimento, a mais de seis meses, na prática específica de uma atividade: dança (n = 10, musculação (n = 10, hidroginástica (n = 12 e caminhada (n = 11. Além disso, um grupo de idosas inativas (n = 13, sem envolvimento em atividade física regular por pelo menos dois meses, também participou do estudo. Foram mensurados o nível de atividade física (Questionário de Baecke, a capacidade funcional (Bateria da AAHPERD e os parâmetros cinemáticos do andar (comprimento da passada e do passo, duração e velocidade da passada, cadência e duração das fases de suporte simples, balanço e duplo suporte. Os resultados revelaram que o nível de atividade física do grupo Controle foi diferente dos demais grupos que praticam atividades físicas. Em relação à capacidade funcional, apenas o componente força apresentou diferenças entre os grupos, indicando que o grupo Controle difere do grupo musculação. Quanto às variáveis do andar, o grupo Controle foi estatisticamente diferente apenas do grupo dança, tanto no comprimento do passo como no comprimento da passada. Pode-se concluir que a capacidade funcional e os parâmetros do andar dos idosos ativos e sedentários apresentam poucas diferenças

  15. Combination with CK19 Might Increase the Prognostic Power of Hep Par 1 in Hepatocellular Carcinoma after Curative Resection.

    Science.gov (United States)

    Jin, Ye; Liang, Zhi-Yong; Zhou, Wei-Xun; Zhou, Li

    2017-07-31

    Hepatocyte Paraffin 1 (Hep Par 1) and cytokeratin 19 (CK19) were shown to be associated with post-surgical prognosis of hepatocellular carcinoma (HCC). However, further validation might be needed. Besides, their combined evaluation has not been reported. The present study was designed to address the issues. Expressions of Hep Par 1 and CK19 were detected using tissue microarray-based immunohistochemical staining in 79 patients with HCC underwent curative hepatectomy. Their associations with cliniopathologic variables, overall and recurrence-free survival were analyzed. Hep Par 1 was highly expressed in 61 patients (77.2%), whereas CK19 was positive in 8 patients (10.1%). Moreover, expressions of these two proteins were all associated with tumor-node-metastasis (TNM) stage and vascular invasion. It was found that high Hep Par 1 expression was univariately associated with good overall and recurrence-free survival, while CK19 was marginally prognostic. Also in univariate analyses, combination of the two markers more effectively predicted for long-term prognosis in HCC than Hep Par 1 did. However, neither Hep Par 1 nor Hep Par 1/CK19 was multivariately significant. Finally, Hep Par 1/CK19 combined with TNM stage might obtain more satisfactory outcome prediction, especially for overall survival. Combination of CK19 with Hep Par 1 might have higher prognostic power, which might be further improved by adding TNM stage, than Hep Par 1 alone, in resected HCC. Of course, subsequent confirmation is necessary.

  16. ParA2, a Vibrio cholerae chromosome partitioning protein, forms left-handed helical filaments on DNA.

    Science.gov (United States)

    Hui, Monica P; Galkin, Vitold E; Yu, Xiong; Stasiak, Alicja Z; Stasiak, Andrzej; Waldor, Matthew K; Egelman, Edward H

    2010-03-01

    Most bacterial chromosomes contain homologs of plasmid partitioning (par) loci. These loci encode ATPases called ParA that are thought to contribute to the mechanical force required for chromosome and plasmid segregation. In Vibrio cholerae, the chromosome II (chrII) par locus is essential for chrII segregation. Here, we found that purified ParA2 had ATPase activities comparable to other ParA homologs, but, unlike many other ParA homologs, did not form high molecular weight complexes in the presence of ATP alone. Instead, formation of high molecular weight ParA2 polymers required DNA. Electron microscopy and three-dimensional reconstruction revealed that ParA2 formed bipolar helical filaments on double-stranded DNA in a sequence-independent manner. These filaments had a distinct change in pitch when ParA2 was polymerized in the presence of ATP versus in the absence of a nucleotide cofactor. Fitting a crystal structure of a ParA protein into our filament reconstruction showed how a dimer of ParA2 binds the DNA. The filaments formed with ATP are left-handed, but surprisingly these filaments exert no topological changes on the right-handed B-DNA to which they are bound. The stoichiometry of binding is one dimer for every eight base pairs, and this determines the geometry of the ParA2 filaments with 4.4 dimers per 120 A pitch left-handed turn. Our findings will be critical for understanding how ParA proteins function in plasmid and chromosome segregation.

  17. Clathrin and LRP-1-independent constitutive endocytosis and recycling of uPAR.

    Directory of Open Access Journals (Sweden)

    Katia Cortese

    Full Text Available BACKGROUND: The urokinase receptor (uPAR/CD87 is highly expressed in malignant tumours. uPAR, as a GPI anchored protein, is preferentially located at the cell surface, where it interacts with its ligands urokinase (uPA and the extracellular matrix protein vitronectin, thus promoting plasmin generation, cell-matrix interactions and intracellular signalling events. Interaction with a complex formed by uPA and its inhibitor PAI-1 induces cell surface down regulation and recycling of the receptor via the clathrin-coated pathway, a process dependent on the association to LRP-1. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we have found that along with the ligand-induced down-regulation, uPAR also internalizes and recycles constitutively through a second pathway that is independent of LRP-1 and clathrin but shares some properties with macropinocytosis. The ligand-independent route is amiloride-sensitive, does not require uPAR partitioning into lipid rafts, is independent of the activity of small GTPases RhoA, Rac1 and Cdc42, and does not require PI3K activity. Constitutively endocytosed uPAR is found in EEA1 positive early/recycling endosomes but does not reach lysosomes in the absence of ligands. Electron microscopy analysis reveals the presence of uPAR in ruffling domains at the cell surface, in macropinosome-like vesicles and in endosomal compartments. CONCLUSIONS/SIGNIFICANCE: These results indicate that, in addition to the ligand-induced endocytosis of uPAR, efficient surface expression and membrane trafficking might also be driven by an uncommon macropinocytic mechanism coupled with rapid recycling to the cell surface.

  18. ParsEval: parallel comparison and analysis of gene structure annotations

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    Standage Daniel S

    2012-08-01

    Full Text Available Abstract Background Accurate gene structure annotation is a fundamental but somewhat elusive goal of genome projects, as witnessed by the fact that (model genomes typically undergo several cycles of re-annotation. In many cases, it is not only different versions of annotations that need to be compared but also different sources of annotation of the same genome, derived from distinct gene prediction workflows. Such comparisons are of interest to annotation providers, prediction software developers, and end-users, who all need to assess what is common and what is different among distinct annotation sources. We developed ParsEval, a software application for pairwise comparison of sets of gene structure annotations. ParsEval calculates several statistics that highlight the similarities and differences between the two sets of annotations provided. These statistics are presented in an aggregate summary report, with additional details provided as individual reports specific to non-overlapping, gene-model-centric genomic loci. Genome browser styled graphics embedded in these reports help visualize the genomic context of the annotations. Output from ParsEval is both easily read and parsed, enabling systematic identification of problematic gene models for subsequent focused analysis. Results ParsEval is capable of analyzing annotations for large eukaryotic genomes on typical desktop or laptop hardware. In comparison to existing methods, ParsEval exhibits a considerable performance improvement, both in terms of runtime and memory consumption. Reports from ParsEval can provide relevant biological insights into the gene structure annotations being compared. Conclusions Implemented in C, ParsEval provides the quickest and most feature-rich solution for genome annotation comparison to date. The source code is freely available (under an ISC license at http://parseval.sourceforge.net/.

  19. Detecting Plant Photoprotective Response to Water Stress Through Variation In PAR Reflectance

    Science.gov (United States)

    Zygielbaum, A. I.; Arkebauer, T. J.; Walter-Shea, E.

    2012-12-01

    Published papers over several decades have shown increasing leaf-level optical reflectance with decreasing leaf water content. Our experimental results using maize and sorghum showed this increase consistently, caused by variation in optical absorption, in the visible (photosynthetically active radiation - PAR) and middle infrared (MIR) spectral regions. Relatively smaller response, driven by variation in optical scatter, was observed in near infrared (NIR). The concomitant increasing reflectance in the PAR and MIR regions is perplexing. PAR reflectance is dominated by chlorophyll absorption while MIR reflectance is dominated by water molecule absorption. However, changes in chlorophyll concentration, determined by chemical extraction, were too small to account for the variation in PAR reflectance. PAR and MIR reflectances were also influenced by the strength of incident light. Hence PAR reflectance appears to be modulated not only by pigment concentration, the classical description, but also by the strength of incident light and the severity of water deficit. We previously reported that these findings were consistent with chloroplast avoidance movement, a plant photoprotective response, which limits light absorption by pigments. We report here our continuing investigation of this phenomenon. In addition to reflectance measurements, time-lapse microscope images of leaves under increasing water deficit conditions were obtained. These show a brightening between veins which strongly supports our assertion that changes in PAR reflectance accompanying water deficit are caused primarily by chloroplast avoidance movement. Our results suggest that leaf, and possibly canopy, reflectance can therefore be used to detect and measure plant stress. These results also indicate that chloroplast avoidance movement may cause poor estimates of leaf chlorophyll content using techniques based on fluoresced, reflected or transmitted light.

  20. Transcription factor network downstream of protease activated receptors (PARs modulating mouse bladder inflammation

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    Hurst Robert E

    2007-08-01

    Full Text Available Abstract Background All four PARs are present in the urinary bladder, and their expression is altered during inflammation. In order to search for therapeutic targets other than the receptors themselves, we set forth to determine TFs downstream of PAR activation in the C57BL/6 urinary bladders. Methods For this purpose, we used a protein/DNA combo array containing 345 different TF consensus sequences. Next, the TF selected was validated by EMSA and IHC. As mast cells seem to play a fundamental role in bladder inflammation, we determined whether c-kit receptor deficient (Kitw/Kitw-v mice have an abrogated response to PAR stimulation. Finally, TFEB antibody was used for CHIP/Q-PCR assay and revealed up-regulation of genes known to be downstream of TFEB. Results TFEB, a member of the MiTF family of basic helix-loop-helix leucine zipper, was the only TF commonly up-regulated by all PAR-APs. IHC results confirm a correlation between inflammation and TFEB expression in C57BL/6 mice. In contrast, Kitw/Kitw-v mice did not exhibit inflammation in response to PAR activation. EMSA results confirmed the increased TFEB binding activity in C57BL/6 but not in Kitw/Kitw-v mice. Conclusion This is the first report describing the increased expression of TFEB in bladder inflammation in response to PAR activation. As TFEB belongs to a family of TFs essential for mast cell survival, our findings suggest that this molecule may influence the participation of mast cells in PAR-mediated inflammation and that targeting TFEB/MiTF activity may be a novel approach for the treatment of bladder inflammatory disorders.

  1. Association of thrombin generation potential with platelet PAR-1 regulation and P-selectin expression in patients on dual antiplatelet therapy.

    Science.gov (United States)

    Badr Eslam, Roza; Posch, Florian; Lang, Irene M; Gremmel, Thomas; Eichelberger, Beate; Ay, Cihan; Panzer, Simon

    2014-02-01

    We studied the association of thrombin generation potential with platelet protease activated receptor (PAR)-1 regulation and platelet activation in 52 stable coronary artery disease patients on continuous therapy with aspirin and clopidogrel (n = 42) or prasugrel (n = 10). Compared to controls, peak thrombin generation potential was elevated in only 11 patients (p > 0.05), while F1.2 was elevated in 26 patients (p P-selectin expression were significantly elevated in patients compared to controls (p P-selectin (p = 0.002), suggesting in vivo depletion of platelet alpha granules due to ongoing platelet activation.

  2. Extaraction par solvant du zinc(II) en milieu acetate par le PhDBP, TBP et D2EHPA. Etude de l'extraction synergique du zinc (II) par les melanges.

    OpenAIRE

    GUEZZEN, Brahim

    2015-01-01

    Aterme de notre étude , les diverses observations tout au long des expériences effectuées nous ont permis de metre en valeur les principales conclusions qui sont les suiveantes : Concernant la synthèse et la caractérisation du phDBP, on retient que le phényl dibutyl phosphate " phDBP" procède par substitution nucléophile entre le dichlorophényl phosphate et le ....

  3. Early intraplatelet signaling enhances the release of human platelet PAR-1 and -4 amino-terminal peptides in response to thrombin.

    Science.gov (United States)

    Ofosu, Frederick A; Dewar, Lori; Song, Yingqi; Cedrone, Aisha C; Hortelano, Gonzalo; Craven, Sharon J

    2009-02-24

    Activation of washed human platelets initiated with alpha-thrombin, SFLLRN, or AYPGKF invariably results in the generation of PAR-1-(1-41) and PAR-4-(1-47). PAR-1-(1-41) and PAR-4-(1-47) are amino-terminal peptides generated when PAR-1 and -4 are cleaved in their first extracellular domains after R(41) and R(47), respectively, to expose the tethered ligand domains of PAR-1 and -4. Since soybean trypsin inhibitor decreases generation of PAR-1-(1-41) and PAR-4-(1-47) and other platelet aggregation-related responses to these three agonists, but does not inactivate alpha-thrombin, a platelet trypsin-like proteinase apparently activates PAR-1 and -4 to propagate PAR-dependent platelet responses. This study identified the signaling pathways implicated in the generation of the platelet proteinase that in turn produces PAR-1-(1-41) and PAR-4-(1-47), to thereby drive the subsequent PAR-dependent platelet aggregation-related responses to alpha-thrombin, SFLLRN, or AYPGKF. Only inhibitors of signaling enzymes that prevented ATP release (forskolin, PGE(1), or BIMI-1) prevented or delayed the generation of PAR-1-(1-41) and PAR-4-(1-47) in response to all three agonists. SBTI prevented platelet aggregation initiated by alpha-thrombin, SFLLRN, or AYPGKF but did so less effectively when it was added 10 s after each agonist. Thus, the platelet-derived proteinase acts within 10 s of each agonist addition to generate PAR-1-(1-41) and PAR-4-(1-47). Furthermore, alpha-thrombin may not effectively catalyze PAR-1-(1-41) and PAR-4-(1-47) generation. We propose that unidentified ATP-dependent phosphorylation reactions catalyzed by PKC help to generate the platelet-derived proteinase that propagates human platelet PAR-1 and -4 activation by the three agonists.

  4. Identification of a new epitope in uPAR as a target for the cancer therapeutic monoclonal antibody ATN-658, a structural homolog of the uPAR binding integrin CD11b (αM.

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    Xiang Xu

    Full Text Available The urokinase plasminogen activator receptor (uPAR plays a role in tumor progression and has been proposed as a target for the treatment of cancer. We recently described the development of a novel humanized monoclonal antibody that targets uPAR and has anti-tumor activity in multiple xenograft animal tumor models. This antibody, ATN-658, does not inhibit ligand binding (i.e. uPA and vitronectin to uPAR and its mechanism of action remains unclear. As a first step in understanding the anti-tumor activity of ATN-658, we set out to identify the epitope on uPAR to which ATN-658 binds. Guided by comparisons between primate and human uPAR, epitope mapping studies were performed using several orthogonal techniques. Systematic site directed and alanine scanning mutagenesis identified the region of aa 268-275 of uPAR as the epitope for ATN-658. No known function has previously been attributed to this epitope Structural insights into epitope recognition were obtained from structural studies of the Fab fragment of ATN-658 bound to uPAR. The structure shows that the ATN-658 binds to the DIII domain of uPAR, close to the C-terminus of the receptor, corroborating the epitope mapping results. Intriguingly, when bound to uPAR, the complementarity determining region (CDR regions of ATN-658 closely mimic the binding regions of the integrin CD11b (αM, a previously identified uPAR ligand thought to be involved in leukocyte rolling, migration and complement fixation with no known role in tumor progression of solid tumors. These studies reveal a new functional epitope on uPAR involved in tumor progression and demonstrate a previously unrecognized strategy for the therapeutic targeting of uPAR.

  5. Transforaminal endoscopic discectomy to relieve sciatica and delay fusion in a 31-year-old man with pars defects and low-grade spondylolisthesis.

    Science.gov (United States)

    Madhavan, Karthik; Chieng, Lee Onn; Hofstetter, Christoph P; Wang, Michael Y

    2016-02-01

    Isthmic spondylolisthesis due to pars defects resulting from trauma or spondylolysis is not uncommon. Symptomatic patients with such pars defects are traditionally treated with a variety of fusion surgeries. The authors present a unique case in which such a patient was successfully treated with endoscopic discectomy without iatrogenic destabilization. A 31-year-old man presented with a history of left radicular leg pain along the distribution of the sciatic nerve. He had a disc herniation at L5/S1 and bilateral pars defects with a Grade I spondylolisthesis. Dynamic radiographic studies did not show significant movement of L-5 over S-1. The patient did not desire to have a fusion. After induction of local anesthesia, the patient underwent an awake transforaminal endoscopic discectomy via the extraforaminal approach, with decompression of the L-5 and S-1 nerve roots. His preoperative pain resolved immediately, and he was discharged home the same day. His preoperative Oswestry Disability Index score was 74, and postoperatively it was noted to be 8. At 2-year follow-up he continued to be symptom free, and no radiographic progression of the listhesis was noted. In this case preservation of stabilizing structures, including the supraspinous and interspinous ligaments and the facet capsule, may have reduced the likelihood of iatrogenic instability while at the same time achieving symptom control. This may be a reasonable option for select patient symptoms confined to lumbosacral radiculopathy.

  6. CFD modelling of hydrogen stratification in enclosures: Model validation and application to PAR performance

    Energy Technology Data Exchange (ETDEWEB)

    Hoyes, J.R., E-mail: james.hoyes@hsl.gsi.gov.uk; Ivings, M.J.

    2016-12-15

    Highlights: • The ability of CFD to predict hydrogen stratification phenomena is investigated. • Contrary to expectation, simulations on tetrahedral meshes under-predict mixing. • Simulations on structured meshes give good agreement with experimental data. • CFD model used to investigate the effects of stratification on PAR performance. • Results show stratification can have a significant effect on PAR performance. - Abstract: Computational Fluid Dynamics (CFD) models are maturing into useful tools for supporting safety analyses. This paper investigates the capabilities of CFD models for predicting hydrogen stratification in a containment vessel using data from the NEA/OECD SETH2 MISTRA experiments. Further simulations are then carried out to illustrate the qualitative effects of hydrogen stratification on the performance of Passive Autocatalytic Recombiner (PAR) units. The MISTRA experiments have well-defined initial and boundary conditions which makes them well suited for use in a validation study. Results are presented for the sensitivity to mesh resolution and mesh type. Whilst the predictions are shown to be largely insensitive to the mesh resolution they are surprisingly sensitive to the mesh type. In particular, tetrahedral meshes are found to induce small unphysical convection currents that result in molecular diffusion and turbulent mixing being under-predicted. This behaviour is not unique to the CFD model used here (ANSYS CFX) and furthermore, it may affect simulations run on other non-aligned meshes (meshes that are not aligned perpendicular to gravity), including non-aligned structured meshes. Following existing best practice guidelines can help to identify potential unphysical predictions, but as an additional precaution consideration should be given to using gravity-aligned meshes for modelling stratified flows. CFD simulations of hydrogen recombination in the Becker Technologies THAI facility are presented with high and low PAR positions

  7. Transcriptome mapping of pAR060302, a blaCMY-2-positive broad-host-range IncA/C plasmid.

    Science.gov (United States)

    Lang, Kevin S; Danzeisen, Jessica L; Xu, Wayne; Johnson, Timothy J

    2012-05-01

    The multidrug resistance-encoding plasmids belonging to the IncA/C incompatibility group have recently emerged among Escherichia coli and Salmonella enterica strains in the United States. These plasmids have a unique genetic structure compared to other enterobacterial plasmid types, a broad host range, and a propensity to acquire large numbers of antimicrobial resistance genes via their accessory regions. Using E. coli strain DH5α harboring the prototype IncA/C plasmid pAR060302, we sought to define the baseline transcriptome of IncA/C plasmids under laboratory growth and in the face of selective pressure. The effects of ampicillin, florfenicol, or streptomycin exposure were compared to those on cells left untreated at logarithmic phase using Illumina platform-based RNA sequencing (RNA-Seq). Under growth in Luria-Bertani broth lacking antibiotics, much of the backbone of pAR060302 was transcriptionally inactive, including its putative transfer regions. A few plasmid backbone genes of interest were highly transcribed, including genes of a putative toxin-antitoxin system and an H-NS-like transcriptional regulator. In contrast, numerous genes within the accessory regions of pAR060302 were highly transcribed, including the resistance genes floR, bla(CMY-2), aadA, and aacA. Treatment with ampicillin or streptomycin resulted in no genes being differentially expressed compared to controls lacking antibiotics, suggesting that many of the resistance-associated genes are not differentially expressed due to exposure to these antibiotics. In contrast, florfenicol treatment resulted in the upregulation of floR and numerous chromosomal genes. Overall, the transcriptome mapping of pAR060302 suggests that it mitigates the fitness costs of carrying resistance-associated genes through global regulation with its transcriptional regulators.

  8. Foix-Chavany-Marie syndrome caused by a disconnection between the right pars opercularis of the inferior frontal gyrus and the supplementary motor area.

    Science.gov (United States)

    Martino, Juan; de Lucas, Enrique Marco; Ibáñez-Plágaro, Francisco Javier; Valle-Folgueral, José Manuel; Vázquez-Barquero, Alfonso

    2012-11-01

    Foix-Chavany-Marie syndrome (FCMS) is a rare type of suprabulbar palsy characterized by an automatic-voluntary dissociation of the orofacial musculature. Here, the authors report an original case of FCMS that occurred intraoperatively while resecting the pars opercularis of the inferior frontal gyrus. This 25-year-old right-handed man with an incidentally diagnosed right frontotemporoinsular tumor underwent surgery using an asleep-awake-asleep technique with direct cortical and subcortical electrical stimulation and a transopercular approach to the insula. While resecting the anterior part of the pars opercularis the patient suffered sudden anarthria and bilateral facial weakness. He was unable to speak or show his teeth on command, but he was able to voluntarily move his upper and lower limbs. This syndrome lasted for 8 days. Postoperative diffusion tensor imaging tractography revealed that connections of the pars opercularis of the right inferior frontal gyrus with the frontal aslant tract (FAT) and arcuate fasciculus (AF) were damaged. This case supplies evidence for localizing the structural substrate of FCMS. It was possible, for the first time in the literature, to accurately correlate the occurrence of FCMS to the resection of connections between the FAT and AF, and the right pars opercularis of the inferior frontal gyrus. The FAT has been recently described, but it may be an important connection to mediate supplementary motor area control of orofacial movement. The present case also contributes to our knowledge of complication avoidance in operculoinsular surgery. A transopercular approach to insuloopercular gliomas can generate FCMS, especially in cases of previous contralateral lesions. The prognosis is favorable, but the patient should be informed of this particular hazard, and the surgeon should anticipate the surgical strategy in case the syndrome occurs intraoperatively in an awake patient.

  9. Cambios en el tono muscular en dos niños con parálisis cerebral espástica mediante la hipoterapia: reporte de casos

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    Yasmín Andrea Rodríguez Laiseca

    2016-08-01

    Full Text Available Introducción: Estudios previos han demostrado que la hipoterapia como terapia complementaria mejora la función motora gruesa en niños con parálisis cerebral. Sin embargo la evidencia no es concluyente. Objetivo: Determinar los cambios en el tono muscular de dos pacientes con parálisis cerebral tipo cuadriparesia espástica mediante la hipoterapia. Descripción del caso: El estudio se llevó a cabo con dos sujetos de género masculino de dos años de edad, con diagnóstico de parálisis cerebral tipo cuadriparesia espástica, nivel V según la escala Gross Motor Function Clasification System (GMFCS. Los niños realizaron 27 sesiones de hipoterapia distribuidas de la siguiente manera: 3 sesiones de acoplamiento en hipoterapia en la primera semana y 24  sesiones de monta terapéutica con una frecuencia de tres veces por semana y una intensidad de 20 minutos por sesión. Se llevó a cabo la valoración del tono muscular mediante la escala de Ashworth modificada (MAS de la siguiente forma: una inicial, dos de control, cuatro post-monta, una final tras 9 semanas de tratamiento continuo y tres mediciones más en la semana uno, dos y tres de inactividad terapéutica. Resultados y discusión: La hipoterapia demostró efecto modulador sobre el tono muscular de los dos sujetos finalizadas las 27 sesiones de hipoterapia, pero este se pierde progresivamente durante el periodo de inactividad terapéutica. Por lo tanto se confirma que esta técnica puede ser una estrategia de tratamiento para el manejo de la espasticidad en pacientes con parálisis cerebral, siempre y cuando se aplique continuamente.

  10. Par polarity complex in mammalian neurogenesis%哺乳动物神经发育中的Par极性复合体

    Institute of Scientific and Technical Information of China (English)

    陈慧灵; 陈晓萍

    2013-01-01

    哺乳动物的神经发育过程极其复杂,其形态结构和机能变化受到严格的调控.细胞极性是哺乳动物神经发生中最基本的特征之一,在其调控因素中,Par极性复合体是研究最多的蛋白质.神经发育过程中Par蛋白的分布与量呈现动态变化,影响细胞连接建立、细胞极性形成、神经突触发生及神经元迁移,也影响到神经前体细胞的命运.文章主要从胚胎新皮层神经前体细胞及体外培养神经元角度,总结了近年在Par极性蛋白的细胞内分布、机能及作用机制方面的研究进展.%Mammalian neurogenesis is a highly complicated process with programmed morphology and function evolution. Polarity, a basic characteristic in neurogenesis, is controlled by regulating proteins such as the partition defective (Par) proteins. Par polarity complex is the most investigated Par protein among these polarity proteins. There are developmental dynamic changes during neurogenesis for the protein distribution and content, which are related to the junction establishment, polarity formation, synaptogenesis, and neuronal migration. Moreover, Par complex is considered to affect the developmental fate of neural precursors. This study summarizes the advances of the protein cellular location, function, and mechanism in embryal neocortex and cultured neuron.

  11. MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.

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    Laura P Bernard

    Full Text Available The Par1 kinases, also known as microtubule affinity-regulating kinases (MARKs, are important for the establishment of cell polarity from worms to mammals. Dysregulation of these kinases has been implicated in autism, Alzheimer's disease and cancer. Despite their important function in health and disease, it has been unclear how the activity of MARK/Par1 is regulated by signals from cell surface receptors. Here we show that MARK/Par1 is activated downstream of NMDA receptors in primary hippocampal neurons. Further, we show that this activation is dependent on protein kinase A (PKA, through the phosphorylation of Ser431 of Par4/LKB1, the major upstream kinase of MARK/Par1. Together, our data reveal a novel mechanism by which MARK/Par1 is activated at the neuronal synapse.

  12. Cortical Polarity of the RING Protein PAR-2 Is Maintained by Exchange Rate Kinetics at the Cortical-Cytoplasmic Boundary

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    Yukinobu Arata

    2016-08-01

    Full Text Available Cell polarity arises through the spatial segregation of polarity regulators. PAR proteins are polarity regulators that localize asymmetrically to two opposing cortical domains. However, it is unclear how the spatially segregated PAR proteins interact to maintain their mutually exclusive partitioning. Here, single-molecule detection analysis in Caenorhabditis elegans embryos reveals that cortical PAR-2 diffuses only short distances, and, as a result, most PAR-2 molecules associate and dissociate from the cortex without crossing into the opposing domain. Our results show that cortical PAR-2 asymmetry is maintained by the local exchange reactions that occur at the cortical-cytoplasmic boundary. Additionally, we demonstrate that local exchange reactions are sufficient to maintain cortical asymmetry in a parameter-free mathematical model. These findings suggest that anterior and posterior PAR proteins primarily interact through the cytoplasmic pool and not via cortical diffusion.

  13. Cortical Polarity of the RING Protein PAR-2 Is Maintained by Exchange Rate Kinetics at the Cortical-Cytoplasmic Boundary.

    Science.gov (United States)

    Arata, Yukinobu; Hiroshima, Michio; Pack, Chan-Gi; Ramanujam, Ravikrishna; Motegi, Fumio; Nakazato, Kenichi; Shindo, Yuki; Wiseman, Paul W; Sawa, Hitoshi; Kobayashi, Tetsuya J; Brandão, Hugo B; Shibata, Tatsuo; Sako, Yasushi

    2016-08-23

    Cell polarity arises through the spatial segregation of polarity regulators. PAR proteins are polarity regulators that localize asymmetrically to two opposing cortical domains. However, it is unclear how the spatially segregated PAR proteins interact to maintain their mutually exclusive partitioning. Here, single-molecule detection analysis in Caenorhabditis elegans embryos reveals that cortical PAR-2 diffuses only short distances, and, as a result, most PAR-2 molecules associate and dissociate from the cortex without crossing into the opposing domain. Our results show that cortical PAR-2 asymmetry is maintained by the local exchange reactions that occur at the cortical-cytoplasmic boundary. Additionally, we demonstrate that local exchange reactions are sufficient to maintain cortical asymmetry in a parameter-free mathematical model. These findings suggest that anterior and posterior PAR proteins primarily interact through the cytoplasmic pool and not via cortical diffusion.

  14. Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology

    Science.gov (United States)

    Bache, Michael; Bosco, Filippo G.; Brøgger, Anna L.; Frøhling, Kasper B.; Sonne Alstrøm, Tommy; Hwu, En-Te; Chen, Ching-Hsiu; Eugen-Olsen, Jesper; Hwang, Ing-Shouh; Boisen, Anja

    2013-11-01

    In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody-antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology. Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.

  15. "It was like reading a detective novel": Using PAR to work together for culture change.

    Science.gov (United States)

    Fortune, Darla; McKeown, Janet; Dupuis, Sherry; de Witt, Lorna

    2015-08-01

    Participatory action research (PAR), with its focus on engagement and collaboration, is uniquely suited to enhancing culture change initiatives in dementia care. Yet, there is limited literature of its application to culture change approaches in care settings, and even less in dementia specific care contexts. To address these gaps in the literature, the purpose of this paper is to examine the complexities of a PAR project aimed at changing the culture of dementia care in two diverse dementia care settings, including a long term care (LTC) and community care setting. Drawing from data gathered throughout the PAR process, we unpack the challenges experienced by participants working together to guide culture change within their respective care settings. These challenges include: overextending selves through culture change participation; fluctuating group membership; feeling uncertainty, confusion and apprehension about the process; frustratingly slow process; and seeking diverse group representation in decision making. We also highlight the potential for appreciative inquiry (AI) to be integrated with PAR to guide a process whereby participants involved in culture change initiatives can develop strategies to mitigate challenges they experience. We view the challenges and strategies shared here as being constructive to would-be culture change agents and hope this paper will move others to consider the use of PAR when engaging in culture change initiatives.

  16. PAR-1 mediated apoptosis of breast cancer cells by V. cholerae hemagglutinin protease.

    Science.gov (United States)

    Ray, Tanusree; Pal, Amit

    2016-05-01

    Bacterial toxins have emerged as promising agents in cancer treatment strategy. Hemagglutinin (HAP) protease secreted by Vibrio cholerae induced apoptosis in breast cancer cells and regresses tumor growth in mice model. The success of novel cancer therapies depends on their selectivity for cancer cells with limited toxicity for normal tissues. Increased expression of Protease Activated Receptor-1 (PAR-1) has been reported in different malignant cells. In this study we report that HAP induced activation and over expression of PAR-1 in breast cancer cells (EAC). Immunoprecipitation studies have shown that HAP specifically binds with PAR-1. HAP mediated activation of PAR-1 caused nuclear translocation of p50-p65 and the phosphorylation of p38 which triggered the activation of NFκB and MAP kinase signaling pathways. These signaling pathways enhanced the cellular ROS level in malignant cells that induced the intrinsic pathway of cell apoptosis. PAR-1 mediated apoptosis by HAP of malignant breast cells without effecting normal healthy cells in the same environment makes it a good therapeutic agent for treatment of cancer.

  17. Two-state conformational equilibrium in the Par-4 leucine zipper domain.

    Science.gov (United States)

    Schwalbe, Martin; Dutta, Kaushik; Libich, David S; Venugopal, Hariprasad; Claridge, Jolyon K; Gell, David A; Mackay, Joel P; Edwards, Patrick J B; Pascal, Steven M

    2010-08-15

    Prostate apoptosis response factor-4 (Par-4) is a pro-apoptotic and tumor-suppressive protein. A highly conserved heptad repeat sequence at the Par-4 C-terminus suggests the presence of a leucine zipper (LZ). This C-terminal region is essential for Par-4 self-association and interaction with various effector proteins. We have used nuclear magnetic resonance (NMR) spectroscopy to fully assign the chemical shift resonances of a peptide comprising the LZ domain of Par-4 at neutral pH. Further, we have investigated the properties of the Par-4 LZ domain and two point mutants under a variety of conditions using NMR, circular dichroism (CD), light scattering, and bioinformatics. Results indicate an environment-dependent conformational equilibrium between a partially ordered monomer (POM) and a predominantly coiled coil dimer (CCD). The combination of techniques used allows the time scales of the equilibrium to be probed and also helps to identify features of the amino acid sequence that may influence the equilibrium.

  18. Valproate alters dopamine signaling in association with induction of Par-4 protein expression.

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    Saebom Lee

    Full Text Available Chromatin remodeling through histone modifications has emerged as a key mechanism in the pathophysiology of psychiatric disorders. Valproate (VPA, a first-line medication for bipolar disorder, is known to have histone deacetylase (HDAC inhibitor activity, but the relationship between its efficacy as a mood stabilizer and HDAC inhibitory activity is unclear. Here we provide evidence that prostate apoptosis response-4 (Par-4, an intracellular binding partner of dopamine D2 receptors (DRD2, plays a role in mediating the effectiveness of VPA. We found that chronic VPA treatment enhanced the expression of Par-4 in cultured neurons and adult mouse brains. This Par-4 induction phenomenon occurred at the transcriptional level and was correlated with an increase in histone H3 and H4 acetylation of the Par-4 promoter regions. Furthermore, chronic VPA treatment potentiated the suppression of the cAMP signaling cascade upon dopamine stimulation, which was blocked by sulpiride treatment. These results indicate that VPA potentiates DRD2 activity by enhancing Par-4 expression via a chromatin remodeling mechanism.

  19. The DNA binding parvulin Par17 is targeted to the mitochondrial matrix by a recently evolved prepeptide uniquely present in Hominidae

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    Bayer Peter

    2007-09-01

    Full Text Available Abstract Background The parvulin-type peptidyl prolyl cis/trans isomerase Par14 is highly conserved in all metazoans. The recently identified parvulin Par17 contains an additional N-terminal domain whose occurrence and function was the focus of the present study. Results Based on the observation that the human genome encodes Par17, but bovine and rodent genomes do not, Par17 exon sequences from 10 different primate species were cloned and sequenced. Par17 is encoded in the genomes of Hominidae species including humans, but is absent from other mammalian species. In contrast to Par14, endogenous Par17 was found in mitochondrial and membrane fractions of human cell lysates. Fluorescence of EGFP fusions of Par17, but not Par14, co-localized with mitochondrial staining. Par14 and Par17 associated with isolated human, rat and yeast mitochondria at low salt concentrations, but only the Par17 mitochondrial association was resistant to higher salt concentrations. Par17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Par17 was shown to bind to double-stranded DNA under physiological salt conditions. Conclusion Taken together, the DNA binding parvulin Par17 is targeted to the mitochondrial matrix by the most recently evolved mitochondrial prepeptide known to date, thus adding a novel protein constituent to the mitochondrial proteome of Hominidae.

  20. The DNA binding parvulin Par17 is targeted to the mitochondrial matrix by a recently evolved prepeptide uniquely present in Hominidae.

    Science.gov (United States)

    Kessler, Daniel; Papatheodorou, Panagiotis; Stratmann, Tina; Dian, Elke Andrea; Hartmann-Fatu, Cristina; Rassow, Joachim; Bayer, Peter; Mueller, Jonathan Wolf

    2007-09-17

    The parvulin-type peptidyl prolyl cis/trans isomerase Par14 is highly conserved in all metazoans. The recently identified parvulin Par17 contains an additional N-terminal domain whose occurrence and function was the focus of the present study. Based on the observation that the human genome encodes Par17, but bovine and rodent genomes do not, Par17 exon sequences from 10 different primate species were cloned and sequenced. Par17 is encoded in the genomes of Hominidae species including humans, but is absent from other mammalian species. In contrast to Par14, endogenous Par17 was found in mitochondrial and membrane fractions of human cell lysates. Fluorescence of EGFP fusions of Par17, but not Par14, co-localized with mitochondrial staining. Par14 and Par17 associated with isolated human, rat and yeast mitochondria at low salt concentrations, but only the Par17 mitochondrial association was resistant to higher salt concentrations. Par17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Par17 was shown to bind to double-stranded DNA under physiological salt conditions. Taken together, the DNA binding parvulin Par17 is targeted to the mitochondrial matrix by the most recently evolved mitochondrial prepeptide known to date, thus adding a novel protein constituent to the mitochondrial proteome of Hominidae.