WorldWideScience

Sample records for consumption ef eletrical

  1. Environmental, financial and energy feasibility of the electrolysis of the water steam of a generation IV reactor cooling system during the moments of low consumption ef eletrical energy: challenges and perspectives

    International Nuclear Information System (INIS)

    Stefanelli, Eduardo J.; Vargas, Miltom; Garcia, Pedro L.; Seo, Emilia S.M.; Oliveira, Wagner de S.

    2009-01-01

    Our civilization is in an inflection moment. Our current decisions will lead us to drastic changes in the planet climate or to cleaner and more sustainable energy generation models. In a certain moment in the evolution of our society, we have privileged the productivity instead of the reasonable use of the planet's resources. This option has been leading us to a situation in which these resources are seriously jeopardized. New models of energy generation and use should be discussed and adopted in order to reverse this process. The electric-power consumption is not constant through time and it must be generated at the moment it is going to be used. There are moments of great idleness in the electric-power generation system, counterbalanced by high demand moments. This characteristic has induced us to the construction of a model of great generation capacity that remains without use most of the time, producing huge financial and environmental impacts. In this article, we discuss the environmental, financial and energy viability of using the idle capacity of the electric-power system to, through water steam electrolysis, produce hydrogen, which would be reconverted into electric power in peak moments by a fuel cell. In this study, we aim at investigating the viability of associating a SOFC (Solid Oxide Fuel Cell), acting as an electrolysis bow, to a generation IV reactor, in order to produce hydrogen from superheated water steam in the cooling of the reactor, which will be converted into electric power via SOFC (Solid Oxide Fuel Cell) in peak moments. The method used in this investigation was to study the electric charge variation consumed in a day, randomly selected in relation to the hour of the day, to launch a curve into a diagram 'Demand x hour of the day', to establish the peak moments, the minimum moments, and the average consumption, and, based on these data and geometrically, predict the viability of using the energetic potential of the moments in which the

  2. Environmental, financial and energy feasibility of the electrolysis of the water steam of a generation IV reactor cooling system during the moments of low consumption ef eletrical energy: challenges and perspectives

    Energy Technology Data Exchange (ETDEWEB)

    Stefanelli, Eduardo J.; Vargas, Miltom; Garcia, Pedro L.; Seo, Emilia S.M.; Oliveira, Wagner de S. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)], e-mail: ipen@stefanelli.eng.br

    2009-07-01

    Our civilization is in an inflection moment. Our current decisions will lead us to drastic changes in the planet climate or to cleaner and more sustainable energy generation models. In a certain moment in the evolution of our society, we have privileged the productivity instead of the reasonable use of the planet's resources. This option has been leading us to a situation in which these resources are seriously jeopardized. New models of energy generation and use should be discussed and adopted in order to reverse this process. The electric-power consumption is not constant through time and it must be generated at the moment it is going to be used. There are moments of great idleness in the electric-power generation system, counterbalanced by high demand moments. This characteristic has induced us to the construction of a model of great generation capacity that remains without use most of the time, producing huge financial and environmental impacts. In this article, we discuss the environmental, financial and energy viability of using the idle capacity of the electric-power system to, through water steam electrolysis, produce hydrogen, which would be reconverted into electric power in peak moments by a fuel cell. In this study, we aim at investigating the viability of associating a SOFC (Solid Oxide Fuel Cell), acting as an electrolysis bow, to a generation IV reactor, in order to produce hydrogen from superheated water steam in the cooling of the reactor, which will be converted into electric power via SOFC (Solid Oxide Fuel Cell) in peak moments. The method used in this investigation was to study the electric charge variation consumed in a day, randomly selected in relation to the hour of the day, to launch a curve into a diagram 'Demand x hour of the day', to establish the peak moments, the minimum moments, and the average consumption, and, based on these data and geometrically, predict the viability of using the energetic potential of the moments

  3. Education for Sustainability (EfS): Practice and Practice Architectures

    Science.gov (United States)

    Kemmis, Stephen; Mutton, Rebecca

    2012-01-01

    This paper reports some findings from an investigation of educational practice in ten (formal and informal) education for sustainability (EfS) initiatives, to characterise exemplary practice in school and community education for sustainability, considered crucial to Australia's future. The study focused on rural/regional Australia, specifically…

  4. Governo eletrônico, reforma do estado e transparência: o governo eletrônico do Brasil

    OpenAIRE

    Prado, Otávio

    2010-01-01

    O objetivo deste trabalho é analisar o programa de governo eletrônico do Brasil, tendo como foco de análise o seu desenvolvimento institucional ao longo dos governos de Fernando Henrique Cardoso e Luiz Inácio Lula da Silva. São ao mesmo tempo analisados os aspectos do programa relacionados à promoção da transparência governamental, de forma a promover a reflexão sobre a importância do governo eletrônico para a democracia. Partindo da hipótese de que o conceito de governo eletrônico é construí...

  5. Direito autoral e o livro eletrônico

    Directory of Open Access Journals (Sweden)

    Helen Beatriz Frota Rozados

    2014-06-01

    Full Text Available Este artigo discorre sobre os novos desafios decorrentes do livro eletrônico no que tange ao direito autoral. Para tanto, utiliza-se de pesquisa bibliográfica para levantar e analisar as características do direito autoral e as propostas de mudanças na legislação brasileira,e descreve as novas formas de proteção desenvolvidas a fim de disponibilizar as obras ao público bem como proteger os direitos autorais. São abordados o Copyleft, Digital Right Management (DRM e o Creative Commons dentre as novas formas de proteção. Conclui-se que o livro eletrônico tem propiciado uma gama de novas discussões no âmbito do mercado editorial e pelos legisladores, levando a uma necessária reflexão, mais intensa, que possa propiciar efetivas mudanças na legislação sobre direito autoral, mudanças estas que englobem um melhor controle do processo de acesso, uso e divulgação, seja para impedir ações ilegais, uso da informação sem a devida creditação, plágio e ou a proliferação de cópias não permitidas.

  6. Cloning and Characterization of EF-Tu and EF-Ts from Pseudomonas aeruginosa

    Directory of Open Access Journals (Sweden)

    Stephanie O. Palmer

    2013-01-01

    Full Text Available We have cloned genes encoding elongation factors EF-Tu and EF-Ts from Pseudomonas aeruginosa and expressed and purified the proteins to greater than 95% homogeneity. Sequence analysis indicated that P. aeruginosa EF-Tu and EF-Ts are 84% and 55% identical to E. coli counterparts, respectively. P. aeruginosa EF-Tu was active when assayed in GDP exchange assays. Kinetic parameters for the interaction of EF-Tu with GDP in the absence of EF-Ts were observed to be = 33 μM, = 0.003 s−1, and the specificity constant was  s−1 μM−1. In the presence of EF-Ts, these values were shifted to = 2 μM, = 0.005 s−1, and the specificity constant was  s−1 μM−1. The equilibrium dissociation constants governing the binding of EF-Tu to GDP ( were 30–75 nM and to GTP ( were 125–200 nM. EF-Ts stimulated the exchange of GDP by EF-Tu 10-fold. P. aeruginosa EF-Tu was active in forming a ternary complex with GTP and aminoacylated tRNA and was functional in poly(U-dependent binding of Phe-tRNAPhe at the A-site of P. aeruginosa ribosomes. P. aeruginosa EF-Tu was active in poly(U-programmed polyphenylalanine protein synthesis system composed of all P. aeruginosa components.

  7. Brownfields/IGD: EF_ACRES

    Data.gov (United States)

    U.S. Environmental Protection Agency — EF_ACRES is a subset of facilities from FRS_INTEREST and FRS_FACILITY_SITE which are updated on a monthly basis as part of the Locational Reference Tables (LRT)...

  8. Hazardous Waste/IGD: EF_RCRA

    Data.gov (United States)

    U.S. Environmental Protection Agency — EF_RCRA is a subset of facilities from FRS_INTEREST and FRS_FACILITY_SITE which are updated on a monthly basis as part of the Locational Reference Tables (LRT)...

  9. Air Emissions/IGD: EF_AFS

    Data.gov (United States)

    U.S. Environmental Protection Agency — EF_AFS is a subset of facilities from FRS_INTEREST and FRS_FACILITY_SITE which are updated on a monthly basis as part of the Locational Reference Tables (LRT)...

  10. Water Dischargers/IGD: EF_NPDES

    Data.gov (United States)

    U.S. Environmental Protection Agency — EF_NPDES is a subset of facilities from FRS_INTEREST and FRS_FACILITY_SITE which are updated on a monthly basis as part of the Locational Reference Tables (LRT)...

  11. Superfund/IGD: EF_NPL

    Data.gov (United States)

    U.S. Environmental Protection Agency — EF_NPL is a subset of facilities from FRS_INTEREST and FRS_FACILITY_SITE which are updated on a monthly basis as part of the Locational Reference Tables (LRT)...

  12. Toxic Releases/IGD: EF_TRI

    Data.gov (United States)

    U.S. Environmental Protection Agency — EF_TRI is a subset of facilities from FRS_INTEREST and FRS_FACILITY_SITE which are updated on a monthly basis as part of the Locational Reference Tables (LRT)...

  13. Heart Failure with Recovered EF and Heart Failure with Mid-Range EF: Current Recommendations and Controversies.

    Science.gov (United States)

    Unkovic, Peter; Basuray, Anupam

    2018-04-03

    This review explores key features and potential management controversies in two emerging populations in heart failure: heart failure with recovered ejection fraction (HF-recovered EF) and heart failure with mid-range ejection fraction (HFmrEF). While HF-recovered EF patients have better outcomes than heart failure with reduced ejection fraction (HFrEF), they continue to have symptoms, persistent biomarker elevations, and abnormal outcomes suggesting a continued disease process. HFmrEF patients appear to have features of HFrEF and heart failure with preserved ejection fraction (HFpEF), but have a high prevalence of ischemic heart disease and may represent a transitory phase between the HFrEF and HFpEF. Management strategies have insufficient data to warrant standardization at this time. HF-recovered EF and HFmrEF represent new populations with unmet needs and expose the pitfalls of an EF basis for heart failure classification.

  14. Propriedades eletrônicas e estruturais de nanotubos de carbono

    OpenAIRE

    Mario Sergio de Carvalho Mazzoni

    1999-01-01

    Nesta tese, empregamos cálculos de primeiros princípios para investigar duas questões relacionadas com propriedades estruturais e eletrônicas de nanotubos de carbono. Na primeira delas, estudamos a influência de deformações mecânicas nas pripriedades eletrônicas dos nanotubos. Especificamente, procuramos responder à pergunta "de que maneira a estrutura eletrônica de um nanotubo é alterada quando ele é dobrado?" Mostramos que a região mais tensionada do tubo deformado comporta-se como um ponto...

  15. A relação entre governo eletrônico e governança eletrônica no governo federal brasileiro

    Directory of Open Access Journals (Sweden)

    Tomás de Aquino Guimarães

    Full Text Available O artigo investiga as contribuições do governo eletrônico para a governança eletrônica no âmbito do governo federal do Brasil. Governança eletrônica é entendida como um processo que visa a manifestação política e a participação da sociedade civil, junto ao governo, por meios eletrônicos, na formulação, acompanhamento da implementação e avaliação das políticas públicas, com vista ao desenvolvimento da cidadania e da democracia. A pesquisa baseou-se na opinião de 27 informantes-chave dos quatro setores que atuam no e-gov: setor público, iniciativa privada, Terceiro Setor e Academia. A análise de conteúdo foi o método utilizado na pesquisa qualitativa para se extrair a percepção dos entrevistados sobre a política de e-gov, tendo sido utilizado o software Atlas.ti para auxiliar na ordenação conceitual dos dados coletados. Concluiu-se que a governança eletrônica, no nível federal, encontra-se em fase inicial de desenvolvimento, com pouca participação dos cidadãos na formulação das políticas públicas, ou seja, uma fraca influência da sociedade civil na tomada de decisão, por meio da Internet, relativa ao estabelecimento da agenda governamental de políticas públicas.

  16. HFpEF and HFrEF Display Different Phenotypes as Assessed by IGF-1 and IGFBP-1.

    Science.gov (United States)

    Faxén, Ulrika Ljung; Hage, Camilla; Benson, Lina; Zabarovskaja, Stanislava; Andreasson, Anna; Donal, Erwan; Daubert, Jean-Claude; Linde, Cecilia; Brismar, Kerstin; Lund, Lars H

    2017-04-01

    Anabolic drive is impaired in heart failure with reduced ejection fraction (HFrEF) but insufficiently studied in heart failure with preserved ejection fraction (HFpEF). Insulin-like growth factor 1 (IGF-1) mediates growth hormone effects and IGF binding protein 1 (IGFBP-1) regulates IGF-1 activity. We tested the hypothesis that HFpEF and HFrEF are similar with regard to IGF-1 and IGFBP-1. In patients with HFpEF (n = 79), HFrEF (n = 85), and controls (n = 136), we analyzed serum IGF-1 and IGFBP-1 concentrations, correlations, and associations with outcome. Age-standardized scores of IGF-1 were higher in HFpEF, median arbitrary units (interquartile range); 1.21 (0.57-1.96) vs HFrEF, 0.09 (-1.40-1.62), and controls, 0.22 (-0.47-0.96), P overall IGF-1 was associated with outcomes in HFrEF, hazard ratio per natural logarithmic increase in IGF-1 SD score 0.51 (95% confidence interval 0.32-0.82, P = .005), but not significantly in HFpEF. IGFBP-1 was not associated with outcomes in either HFpEF nor HFrEF. HFpEF and HFrEF phenotypes were similar with regard to increased IGFBP-1 concentrations but differed regarding IGF-1 levels and prognostic role. HFrEF and HFpEF may display different impairment in anabolic drive. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. Marketing da informação eletrônica

    OpenAIRE

    Amaral, Sueli Angélica do

    1994-01-01

    Trata as características da informação eletrônica, enfatizando as bases de dados. Descreve o mercado desse tipo de informação, estratégias e atividades de marketing aplicadas à informação eletrônica. Destaca a educação dos usuários para realçar o valor dessa informação e melhor aproveitar os recursos e o potencial das novas tecnologias disponíveis. ________________________________________________________________________________________________________________ ABSTRACT ...

  18. The Potential of Devices for HFpEF

    Directory of Open Access Journals (Sweden)

    Andrew J Stewart Coats

    2017-01-01

    Full Text Available The 2016 ESC/HFA HF guidelines list the very many drug and device therapies that are proven to be beneficial in terms of life prolongation and hospitalisation prevention for HFrEF. The list of recommended therapies or devices for HFpEF and HFmrEF is limited to diuretics and the management of comorbidities and so far there is no clinical evidence for an electrical or mechanical device for HFpEF. The Cardiac Contractility Modulation (CCM device that may have a role in selected HFrEF and HFmrEF patients has been reviewed in another paper in this issue but here we survey the results so far of a novel device for patients with HFpEF. Despite lagging many years behind HFrEF, HFpEF patients are now being targeted with novel devices with the potential to improve symptoms, improve quality of life, reduce hospitalization and delay progression of the syndrome. The front-runner is a novel inter-atrial left atrial pressure decompression device the Corvia IASD, which currently enrolling patients in a randomized trial. This device is a CE-marked investigational, non- surgical transcatheter implant designed to provide continuous and dynamic decompression of the left atrium, in an effort to reduce symptoms, HF hospitalizations, and improve quality of life as well as potentially slowing the progression of HFpEF.

  19. Filmes e dispositivos de polímeros eletrônicos isolantes e semicondutivos impressos via sistema de injeção eletrônica.

    OpenAIRE

    Barbosa, Adriana Diniz

    2014-01-01

    Programa de Pós-Graduação em Engenharia de Materiais. Rede Temática em Engenharia de Materiais, Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade Federal de Ouro Preto. Esta tese teve por objetivo principal a fabricação de capacitores, indutores, resistores e sensores de amônia por meio de uma nova técnica de impressão baseada em sistema automotivo de injeção eletrônica. Para tanto, filmes finos de polianilina (PAni), poliestireno (PS) e Ecoflex® foram obtidos utilizando-se um equipam...

  20. Pneumonia cases following an EF-5 tornado.

    Science.gov (United States)

    Forshee-Hakala, Beth A

    2015-07-01

    Infections following a natural disaster such as an EF-5 tornado can be atypical and difficult to treat. Studies have looked at illness following several natural disasters, but few have studied respiratory illness following a tornado. A review of patients with pneumonia admitted during the period from May 22, 2009, through May 21, 2012, was completed. The Tornado Zone Group included adult patients who lived or worked in the tornado zone during the year following the tornado. Data were isolated by number of pneumonia cases within and outside the tornado zone per month per year. An analysis of variance comparing the number of pneumonia cases from the tornado zone per month per year was significant at F2,38 = 12.93 and P Tornado Zone Group (P Tornado Zone patients to be younger than controls (t390 = 5.14; P Tornado Zone Group included uncommon pathogens not isolated during the 2 years prior. The number of pneumonia cases may increase following tornadoes. Although current guidelines recommend narrow-spectrum antibiotics for community-acquired pneumonia, results of this study suggest the possible need for broader antimicrobial coverage after tornadoes. Copyright © 2015 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

  1. Governo eletrônico na mídia on line

    OpenAIRE

    Willecke, Robert

    2003-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Produção. A presente pesquisa teve o objetivo geral de avaliar a repercussão nos meios de comunicação das ações governamentais relacionadas ao processo de implantação do governo eletrônico. Como objetivos específicos, buscou-se identificar as publicações com maior volume e freqüência sobre governo eletrônico, os assuntos e instâncias governamentais com maior desta...

  2. Ecological Footprint Analysis Based on Changing Food Consumption in a Poorly Developed Area of China

    Directory of Open Access Journals (Sweden)

    Lin Zhen

    2017-08-01

    Full Text Available The per capita ecological footprint (EF is a useful tool to compare consumption with nature's ability to support this consumption. Guyuan is an economically impoverished region in China, where EF provides important insights into whether human consumption can be sustained by the local per capita biological capacity (BC, which represents the environment’s ability to support resource use. We estimated the EF of food consumption using local equivalence and yield factors, and compared EF in 1998 and 2013 with BC, which represented the existing biologically productive area (including cultivated land, grassland, forest, and water bodies that supports this consumption. Data were collected from household surveys, government statistics, and land use maps. We found that food consumption changed, with decreasing consumption of staple foods and increasing consumption of meat, eggs, milk, edible oils, fruit, and vegetables. Decreased staple food consumption decreased the EF for this food group, but the large increase in meat consumption greatly increased EF from meat production (to more than 41 times the 1998 value. Cultivated land contributed greatly to both EF and BC, and staple foods and vegetables were the main EF components for this land. Overall, EF from food consumption decreased from 1998 to 2013, but local BC remained 188,356 ha below EF (i.e., current consumption is not sustainable based on local resources. The Grain for Green program, which focuses on increasing the BC of forest and grassland by replacing degraded cultivated land with these land use types, decreased the BC of cultivated land, leading to wide spatial variation in both EF and BC. These results will inform policy development by revealing the condition of each region’s use of the locally available production resources.

  3. Embedding EfS in Teacher Education through a Multi-Level Systems Approach: Lessons from Queensland

    Science.gov (United States)

    Evans, Neus; Ferreira, Jo-Anne; Davis, Julie; Stevenson, Robert B.

    2016-01-01

    This article reports on the fourth stage of an evolving study to develop a systems model for embedding education for sustainability (EfS) into preservice teacher education. The fourth stage trialled the extension of the model to a comprehensive state-wide systems approach involving representatives from all eight Queensland teacher education…

  4. Prevalence of Prediabetes and Undiagnosed Diabetes in Patients with HFpEF and HFrEF and Associated Clinical Outcomes

    DEFF Research Database (Denmark)

    Kristensen, Søren L; Jhund, Pardeep S; Lee, Matthew M Y

    2017-01-01

    PURPOSE: The prevalence and consequences of prediabetic dysglycemia and undiagnosed diabetes is unknown in patients with heart failure (HF) and preserved ejection fraction (HFpEF) and has not been compared to heart failure and reduced ejection fraction (HFrEF). METHODS: We examined the prevalence...... and outcomes associated with normoglycemia, prediabetic dysglycemia and diabetes (diagnosed and undiagnosed) among individuals with a baseline glycated hemoglobin (hemoglobin A1c, HbA1c) measurement stratified by HFrEF or HFpEF in the Candesartan in Heart failure Assessment of Reduction in Mortality...... and was available in 1072/3023 (35%) of patients with HFpEF and 1578/4576 (34%) patients with HFrEF. 18 and 16% had normoglycemia (HbA1c prediabetes (HbA1c 6.0-6.4), respectively. Finally among patients with HFpEF 22% had undiagnosed diabetes (HbA1c > 6.4), and 40% had known diabetes (any Hb...

  5. Névoas, vapores e outras volatilidades ilusórias dos cigarros eletrônicos

    Directory of Open Access Journals (Sweden)

    Liz Maria de Almeida

    2017-09-01

    Full Text Available Resumo: Neste artigo, analisa-se o discurso de fornecedores de cigarros eletrônicos para convencer potenciais usuários (fumantes, ex-fumantes ou nunca fumantes de cigarros a adquirir e usar o novo produto. É um estudo qualitativo, descritivo e exploratório acerca do discurso de vendedores em oito sites de venda on-line que, entre 2011 e 2013, tiveram maior frequência de buscas na Internet. Os sites foram identificados pela ferramenta Google Trends, com base no número de acessos e frequência de buscas ao tema cigarro eletrônico. Tendo como referencial metodológico a hermenêutica-dialética, a categorização do material empírico sob o esquema “compreensão/interpretação” apontou quatro abrangentes sentidos: apropriação do discurso antitabagista; comparação entre cigarros convencional e eletrônico; apelo à crença na fidedignidade da ciência; e projeção da imagem do cigarro eletrônico. A análise desses sentidos configurou os elementos argumentativos do discurso de posicionamento de marketing utilizado por fabricantes e fornecedores de cigarros eletrônicos.

  6. Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis.

    Science.gov (United States)

    Deng, Zhao; Luo, Pei; Lai, Wen; Song, Tongxing; Peng, Jian; Wei, Hong-Kui

    2017-12-09

    Growth of skeletal muscle is dependent on the protein synthesis, and the rate of protein synthesis is mainly regulated in the stage of translation initiation and elongation. Myostatin, a member of the transforming growth factor-β (TGF-β) superfamily, is a negative regulator of protein synthesis. C2C12 myotubes was incubated with 0, 0.01, 0.1, 1, 2, 3 μg/mL myostatin recombinant protein, and then we detected the rates of protein synthesis by the method of SUnSET. We found that high concentrations of myostatin (2 and 3 μg/mL) inhibited protein synthesis by blocking mTOR and eEF2K-eEF2 pathway, while low concentration of myostatin (0.01, 0.1 and 1 μg/mL) regulated eEF2K-eEF2 pathway activity to block protein synthesis without affected mTOR pathway, and myostatin inhibited eEF2K-eEF2 pathway through regulating AMPK pathway to suppress protein synthesis. It provided a new mechanism for myostatin regulating protein synthesis and treating muscle atrophy. Copyright © 2017. Published by Elsevier Inc.

  7. Guerra fiscal: ICMS e o comércio eletrônico

    OpenAIRE

    Maki Minato

    2015-01-01

    Este trabalho tem por objetivo analisar as disputas entre os estados envolvendo a tributação pelo ICMS das operações de circulação de mercadoria contratadas pela internet. A despeito da extensa disciplina sobre o tributo na Constituição Federal de 1988, intensificam-se os conflitos entre os estados em torno do ICMS. No caso do comércio eletrônico, a internet impulsiona o aumento das operações de compra e venda realizadas de forma remota. Tendo em vista que o parâmetro de origem foi adotado pe...

  8. A Imunidade Tributária Aplicada aos Livros Eletrônicos e Ereaders

    OpenAIRE

    Carneiro, Thiago Lima; Meneses, Karina da Silva

    2016-01-01

    A Constituição Federal de 1988 determina a imunidade tributária dos livros e do papel destinado à sua produção, a fim de difundir o conhecimento e garantir a liberdade de expressão. Ocorre que o desenvolvimento tecnológico acarretou o surgimento de livros eletrônicos e aparelhos destinados à sua leitura, os chamados e-readers. Diante desse novo paradigma, resta necessário reavaliar o texto constitucional a fim de mantê-lo atualizado. A jurisprudência brasileira não é uníssona quanto ao assunt...

  9. Implementação Eletrônica de Sistemas Fuzzy.

    OpenAIRE

    André Luís Jorge Garcia

    2009-01-01

    Este trabalho investiga a implementação de sistemas fuzzy com circuitos eletrônicos. Tais sistemas têm demonstrado sua capacidade de resolver diversos tipos de problemas em várias aplicações de engenharia, em especial nas relacionadas com controle de processos. Para processos mais complexos, o raciocínio aproximado da lógica fuzzy fornece uma maneira de compreender o comportamento do sistema, permitindo a interpolação aproximada entre situações observadas de entrada e saída. A ...

  10. O hipertexto eletrônico como base para reconfigurar a atividade jornalística

    Directory of Open Access Journals (Sweden)

    Angèle Murad

    2001-01-01

    Full Text Available

    Trabalho exploratório objetiva analisar como a ferramenta do hipertexto cria as bases para reconfigurar a atividade jornalística, ao proporcionar novas práticas de leitura e escrita, com o favorecimento à intertextualidade e à nova relação entre autor-obra-leitor. As potencialidades e os questionamentos que advêm da aplicação do hipertexto eletrônico para o jornalismo, bem como experiências em curso na rede, revelam a urgência de se realizar pesquisas aplicadas para analisar as práticas.

  11. O comércio eletrônico e as novas formas de informação

    OpenAIRE

    Melo, Marco Antônio Machado Ferreira de

    2002-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Produção. O livro convencional e o eletrônico e as possíveis mudanças, dentro do comércio eletrônico, constituem-se no tema central deste trabalho. O estudo envolve importantes proposições relacionados ao comércio eletrônico, enfatizando as inovações oriundas da tecnologia de comunicação e seus reflexos no modus operandi humano, suas conseqüências e as novas forma...

  12. Protocolo eletrônico multiprofissional de doenças oftalmológicas com ênfase em estrabismo

    OpenAIRE

    Ribeiro, Christie Graf

    2012-01-01

    Resumo: Introdução: A necessidade de criação e desenvolvimento de protocolos eletrônicos para auxílio na pesquisa científica acompanha a realidade da constante evolução médica. Atualmente existem protocolos eletrônicos desenvolvidos e incorporados ao SINPE© nas áreas de cirurgia do aparelho digestivo, urologia, oftalmologia, enfermagem e gestão. Outros protocolos estão em desenvolvimento nas áreas de cirurgia bariátrica, ortopedia, nutrição, anestesiologia e cirurgia plástica. Na área da ofta...

  13. 75 FR 27986 - Electronic Filing System-Web (EFS-Web) Contingency Option

    Science.gov (United States)

    2010-05-19

    ...] Electronic Filing System--Web (EFS-Web) Contingency Option AGENCY: United States Patent and Trademark Office... contingency option when the primary portal to EFS-Web has an unscheduled outage. Previously, the entire EFS-Web system is not available to the users during such an outage. The contingency option in EFS-Web will...

  14. POTENTIAL MARKETING PLAN FOR EF, REGARDING LANGUAGE COURSES IN CHINA

    OpenAIRE

    Saarni, Helena

    2017-01-01

    This bachelor’s thesis was conducted as a potential marketing plan for EF. The aim and purpose of the project was to create a marketing plan for a company called EF regarding their Chinese language courses in China, where they offer these courses to young adults already. These courses however are not marketed in Finland as much as the other language courses. The theoretical part was gathered by collecting information and theories from various sources including books and e-books about mark...

  15. Contribution of effluent organic matter (EfOM) to ultrafiltration (UF) membrane fouling: Isolation, characterization, and fouling effect of EfOM fractions

    KAUST Repository

    Zheng, Xing; Khan, Muhammad; Croue, Jean-Philippe

    2014-01-01

    EfOM has been regarded as a major organic foulant resulting in UF membrane fouling in wastewater reclamation. To investigate fouling potential of different EfOM fractions, the present study isolated EfOM into hydrophobic neutrals (HPO-N), colloids

  16. Exigências Ambientais Europeias: Novos Desafios Competitivos para o Complexo Eletrônico Brasileiro

    Directory of Open Access Journals (Sweden)

    Stela Luiza de Mattos Ansanelli

    2011-05-01

    Full Text Available O objetivo deste artigo é avaliar as implicações das exigências ambientais europeias para equipamentos eletroeletrônicos sobre o complexo eletrônico brasileiro. Essas determinações, que tratam da eliminação de substâncias perigosas e da gestão dos resíduos de produtos eletrônicos, têm atingido não só as empresas do setor em nível internacional, mas também o complexo eletrônico brasileiro. A maior parte das empresas do setor no Brasil está se adequando ao requisito que restringe o uso de substâncias perigosas. Os principais efeitos desse processo são o desenvolvimento de inovações tecnológicas e alterações nas relações contratuais entre as empresas do setor. As filiais estrangeiras estão mais avançadas do que as nacionais quanto ao período de ajuste às novas regras e ao desempenho tecnológico alcançado, sinalizando um alinhamento com as estratégias das matrizes. O descumprimento dessas exigências pelas empresas nacionais evita ganhos comerciais e gera indagações quanto às políticas que devem ser adotadas pelo país.The objective of this paper is to evaluate the implications of the european environmental requirements for electronic equipment on brazilian electronic industry sector. These determinations deal with elimination of hazardous substances and management of electronic products waste and have reached not only the electronic sector companies in international level, but brazilian electronic sector as well. Most brazilian companies of the electronic sector are getting complied with the requirement that restricts the use of hazardous substances. The main consequences of this process are technological innovations and changes on contractual relationships among companies. Foreign companies branches are more advanced than local ones in terms of timing of compliance to the new requirements and technological performance, showing an alignment with headquarter strategies. Not being complied with these

  17. Proposta de um modelo de maturidade para sítios de governo eletrônico

    Directory of Open Access Journals (Sweden)

    Rafael de Mello Lechakoski

    2015-09-01

    Full Text Available Introdução: Tendo em vista a crescente utilização da tecnologia da informação - em especial a internet - pelo governo como meio de interação com cidadãos, a investigação apresenta estudo bibliográfico acerca de modelos de maturidade para governo eletrônico, abordando os temas de serviços públicos e de maturidade tanto para conceituar a Maturidade em Governo Eletrônico como para propor um modelo de maturidade para sítios de governo eletrônico. Método: Para atendimento ao propósito da pesquisa efetuou-se coleta intencional de 24 modelos de maturidade para governo eletrônico e se procedeu a análise comparativa entre eles verificando-se suas limitações em relação aos serviços prestados; à acessibilidade; à usabilidade e à integração com redes sociais, considerando-se a realidade brasileira e os requisitos previstos no Modelo de Acessibilidade em Governo Eletrônico (e-MAG. Resultados: Propõe-se um modelo de maturidade para sítios de governo eletrônico baseado na convergência dos modelos analisados, o qual é composto por quatro estágios voltados à avaliação de requisitos de serviços, acessibilidade, usabilidade e redes sociais. Diferentemente dos demais analisados, o modelo apresentado considera tanto as recomendações de acessibilidade para internet do World Wide Web Consortium (W3C, como aquelas de acessibilidade e usabilidade do governo brasileiro (e-MAG. Em cada estágio proposto apresentam-se exemplos de requisitos que podem ser avaliados e, potencialmente, transformados em indicadores. Conclusão: A utilização de um modelo para avaliar a maturidade em sítios de governo eletrônico evidencia a situação em que o sítio se encontra em relação ao que é esperado em cada estágio e qual é a linha conceitual de evolução dentro do modelo de maturidade, que reflete em melhorias a seus usuários ou cidadãos de maneira geral.

  18. Contrast in atomically resolved EF-SCEM imaging

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Peng [National Laboratory of Solid State Microstructures and Department of Materials Science and Engineering, Nanjing University, Nanjing 210093 (China); D’Alfonso, Adrian J. [School of Physics, University of Melbourne, Parkville, Victoria 3010 (Australia); Hashimoto, Ayako [Surface Physics and Structure Unit, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba 305-0047 (Japan); Global Research Center for Environment and Energy based on Nanomaterials Science, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, 305-0047 (Japan); Electron Microscopy Station, National Institute for Materials Science, 3-13 Sakura, Tsukuba 305-0003 (Japan); Morgan, Andrew J. [School of Physics, University of Melbourne, Parkville, Victoria 3010 (Australia); Takeguchi, Masaki [Surface Physics and Structure Unit, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba 305-0047 (Japan); Electron Microscopy Station, National Institute for Materials Science, 3-13 Sakura, Tsukuba 305-0003 (Japan); Mitsuishi, Kazutaka [Surface Physics and Structure Unit, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba 305-0047 (Japan); Global Research Center for Environment and Energy based on Nanomaterials Science, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, 305-0047 (Japan); Electron Microscopy Station, National Institute for Materials Science, 3-13 Sakura, Tsukuba 305-0003 (Japan); Shimojo, Masayuki [Department of Materials Science and Engineering, Shibaura Institute of Technology, 3-7-5, Toyosu, Koto-ku, Tokyo, 135-8548 (Japan); Kirkland, Angus I. [Department of Materials, University of Oxford, Parks Road, Oxford OX1 3PH (United Kingdom); Allen, Leslie J. [School of Physics, University of Melbourne, Parkville, Victoria 3010 (Australia); Nellist, Peter D., E-mail: peter.nellist@materials.ox.ac.uk [Department of Materials, University of Oxford, Parks Road, Oxford OX1 3PH (United Kingdom)

    2013-11-15

    Energy-filtered scanning confocal electron microscopy (EF-SCEM) is a technique that uses the reduced depth of field of an aberration-corrected transmission electron microscope to provide three-dimensional (3D) compositional information. Using a silicon sample in the <110> orientation, we show that EF-SCEM image data can be recorded that shows lattice resolution in the plane perpendicular to the incident beam direction. The confocal effect is demonstrated through the reduction of the mean intensity as the confocal plane is displaced from the sample mid-plane, unlike optical sectioning in high-angle annular dark-field scanning transmission electron microscopy (STEM). Simulations of the EF-SCEM data show agreement with the experimental data, and allow the interpretability of the data to be explored. The effects of channelling, absorption and delocalisation complicate the quantitative and qualitative interpretation of the data, highlighting the need for matching to simulations. Finally the effects of the finite detector pin-hole aperture size are explored, and we show that the EF-SCEM contrast in the plane perpendicular to the beam direction starts to resemble that of a STEM spectrum imaging experiment as the aperture size increases. - Highlights: • Atomically resolved energy-filtered scanning confocal electron microscopy (EF-SCEM) is demonstrated. • The confocal effect is demonstrated through the reduction of the mean intensity as the confocal plane is displaced from the sample mid-plane. • Simulations show agreement with the experimental data. • The effects of channelling, absorption and delocalisation complicate the quantitative and qualitative interpretation of the data. • The effects of the finite detector pin-hole aperture size are explored.

  19. Contrast in atomically resolved EF-SCEM imaging

    International Nuclear Information System (INIS)

    Wang, Peng; D’Alfonso, Adrian J.; Hashimoto, Ayako; Morgan, Andrew J.; Takeguchi, Masaki; Mitsuishi, Kazutaka; Shimojo, Masayuki; Kirkland, Angus I.; Allen, Leslie J.; Nellist, Peter D.

    2013-01-01

    Energy-filtered scanning confocal electron microscopy (EF-SCEM) is a technique that uses the reduced depth of field of an aberration-corrected transmission electron microscope to provide three-dimensional (3D) compositional information. Using a silicon sample in the orientation, we show that EF-SCEM image data can be recorded that shows lattice resolution in the plane perpendicular to the incident beam direction. The confocal effect is demonstrated through the reduction of the mean intensity as the confocal plane is displaced from the sample mid-plane, unlike optical sectioning in high-angle annular dark-field scanning transmission electron microscopy (STEM). Simulations of the EF-SCEM data show agreement with the experimental data, and allow the interpretability of the data to be explored. The effects of channelling, absorption and delocalisation complicate the quantitative and qualitative interpretation of the data, highlighting the need for matching to simulations. Finally the effects of the finite detector pin-hole aperture size are explored, and we show that the EF-SCEM contrast in the plane perpendicular to the beam direction starts to resemble that of a STEM spectrum imaging experiment as the aperture size increases. - Highlights: • Atomically resolved energy-filtered scanning confocal electron microscopy (EF-SCEM) is demonstrated. • The confocal effect is demonstrated through the reduction of the mean intensity as the confocal plane is displaced from the sample mid-plane. • Simulations show agreement with the experimental data. • The effects of channelling, absorption and delocalisation complicate the quantitative and qualitative interpretation of the data. • The effects of the finite detector pin-hole aperture size are explored

  20. Tron e a construção de um mundo eletrônico

    Directory of Open Access Journals (Sweden)

    Carolina Figueiredo

    2011-12-01

    Full Text Available No início dos anos 80, período de grande desenvolvimento da informática, começam a surgir as pri-meiras representações artísticas do ciberespaço. No cinema, o filme Tron (1982 é pioneiro a construir o mundo eletrônico, dando-lhe formas e cores. O presente artigo procura analisar esta construção e suas relações com a narrativa fílmica, assim como seus desdobramentos em Matrix (1999 e Tron Legacy (2010. Para isso, usa-se como base o artigo Tron: uma representação pioneira do ciberespaço (FIGUEIREDO, 2011, ampliando algumas de suas considerações.

  1. Cibercultura, comunidades virtuais e ‘fetiches eletrônicos’

    Directory of Open Access Journals (Sweden)

    Maria Vandete Almeida

    2012-10-01

    Full Text Available Em meio a um mundo repleto de tecnologias e sob o regime de uma organização social cuja denominação, Sociedade da Informação, traduz e denuncia todo um processo de transição e de transformação dos meios e modos de produção, busca-se nesse trabalho breve reflexão acerca do contexto da sociedade industrial contemporânea que caracterize aculturação no contexto das redes eletrônicas de comunicação e a constituição da ‘consciência coisificada’ em decorrência do ‘fetiche pela técnica’.

  2. Miopatia nemalinica: relato de um caso com estudo histoquimico e microscopia eletrônica

    Directory of Open Access Journals (Sweden)

    Lineu Cesar Werneck

    1983-06-01

    Full Text Available Relato do caso de criança do sexo masculino, com hipotonia e dificuldades de deglutição desde os primeiros dias de vida, necessitando gastrostomia permanente. Apresentava retardo no desenvolvimento motor. A eletromiografia sugeria envolvimento muscular primário e a biópsia muscular, processada a fresco, histoquímica e pela microscopia eletrônica, revelou corpos nemalínicos clássicos e deficiência de fibras do tipo II. São feitos comentários a respeito dos sintomas e formas clínicas, origem e patogenia dos corpos nemalínicos, sendo o caso relatado caracterizado como miopatia nemalínica do tipo congênito.

  3. Breves anotações sobre o comércio eletrônico

    OpenAIRE

    Canella, Sérgio Eduardo; Universidade Estadual de Londrina; Lewis, Sandra Barbon; Universidade Estadual de Londrina

    2005-01-01

    O estudo verifica aspectos da concentração empresarial no comércio eletrônico. Para tanto, primeiramente, é realizada uma análise do papel das empresas no contexto global a partir do avanço tecnológico que é posto à disposição e da nova ordem mundial que lhe é apresentada. O fluxo instantâneo de informações, possibilitado pelo avanço da Internet e o alto grau de competitividade introduzido pela Globalização, levaram os agentes econômicos a buscar no comércio pela Internet uma nova perspectiva...

  4. Protocolo eletrônico multiprofissional em cirurgia bariátrica

    OpenAIRE

    Nassif,Denise Serpa Bopp; Malafaia,Osvaldo; Nassif,Paulo Afonso Nunes; Kuretzki,Carlos Henrique; Lucas,Ricardo Wallace das Chagas; Pinto,José Simão de Paula

    2011-01-01

    RACIONAL: A obesidade é doença multifatorial. A coleta de dados exerce papel fundamental para realização de pesquisas de alto grau de qualidade e, na atualidade, a cirurgia da obesidade carece de meios para realização de pesquisas prospectivas com elevado nível de confiabilidade. Os dados obtidos em protocolos eletrônicos são mais completos, têm poucos erros, são mais consistentes e apresentam baixo percentual de violação em relação aos prontuários de papel. OBJETIVO: 1) Criar base teórica de...

  5. Gestão de odores: fundamentos do Nariz Eletrônico Odor management: fundamentals of Electronic Nose

    Directory of Open Access Journals (Sweden)

    Henrique de Melo Lisboa

    2009-03-01

    Full Text Available Narizes Eletrônicos têm sido desenvolvidos para detecção automática e classificação de odores, vapores e gases. São instrumentos capazes de medir a concentração ou intensidade odorante de modo similar a um olfatômetro, mas sem as limitações inerentes ao uso de painéis humanos, o que é altamente desejável. Um Nariz Eletrônico é geralmente composto por um sistema de sensores químicos e um sistema eletrônico associado à inteligência artificial para reconhecimento. Têm sido aplicados em muitas áreas, tais como análise de alimentos, controles ambientais e diagnósticos médicos. Do ponto de vista ambiental, sistemas de Narizes Eletrônicos vêm sendo usados para monitorar a qualidade do ar, detectar fontes e quantificar emissões odorantes. Este artigo pretende apresentar os fundamentos dos Narizes Eletrônicos.Electronic noses have been developed for automatic detection and classification of odors, vapors and gases. They are instruments capable to identify odors as the human nose does, and measure the odor concentration or intensity according to similar metrics as an olfactometer, but without the inherent limitations of human panels. An Electronic Nose is generally composed of a matrix of chemical sensors and computer based system for odor recognition and classification. It has been applied in many areas, such as food quality analysis, explosives detection, environmental monitoring and medical diagnosis. In the ambient environment, systems of Electronic Noses have been used to monitor the quality of air and to detect and quantify odor sources and emissions. This article intends to present the fundamentals and main characteristics of Electronic Noses.

  6. Pesquisa e desenvolvimento em cerâmicas eletro-eletrônicas no IPEN

    Directory of Open Access Journals (Sweden)

    Muccillo R.

    1999-01-01

    Full Text Available O Grupo de Cerâmicas Eletro-eletrônicas do IPEN desenvolve trabalhos de pesquisa em materiais cerâmicos avançados para utilização em dispositivos sensores de espécies químicas e em células de combustíveis a eletrólitos sólidos. As principais áreas de atuação do grupo são em 1 síntese, processamento e caracterização elétrica de materiais cerâmicos, 2 estudo de correlação microestrutura-propriedades elétricas de materiais cerâmicos, 3 projeto, desenvolvimento, montagem e testes de sensores eletroquímicos de espécies químicas. Os principais materiais estudados são condutores iônicos (à base de ZrO2, ThO2 e outros, protônicos (à base de BaCeO3, varistores à base de SnO2, supercondutores cerâmicos de alta temperatura crítica (das famílias Y-Ba-Cu-O e Bi-Sr-Ca-Cu-O e compósitos de matriz cerâmica (dos tipos isolante em matriz condutora iônica e condutor iônico em matriz supercondutora. A infra-estrutura experimental dispõe de analisador de impedância, difratômetro de raios X, equipamento de análise térmica simultânea, fornos para sinterização e laboratório químico. O trabalho de pesquisa do Grupo é financiado por projetos FAPESP e PRONEX.

  7. Verificação da e-TailQ como instrumento para mensurar a qualidade no varejo eletrônico

    OpenAIRE

    Vieira, Valter Afonso

    2008-01-01

    Para entender a percepção do consumidor quanto ao varejo eletrônico em sua dimensionalidade, este artigo objetiva (a) verificar as propriedades psicométricas do instrumento e-TailQ no varejo eletrônico brasileiro e (b) identificar a associação dos fatores da escala com variáveis de marketing. A amostra é composta por 344 pessoas que já compraram bens no varejo eletrônico, e é configurada como uma survey do tipo bola de neve por conveniência. Os resultados demonstraram que o subconstruto quali...

  8. After the storm: personal experiences following an EF4 tornado.

    Science.gov (United States)

    May, Olivia W; Bigham, Amy B

    2012-08-01

    In April of 2011, an EF4 tornado ripped through the city of Tuscaloosa, AL, leaving in its wake thousands of destroyed homes and businesses. In the hours and days that followed, the health care community of this city, as well as the entire state of Alabama and the southeastern United States, came together to provide care to hundreds of victims, recovery workers, and volunteers. Copyright © 2012 Elsevier Inc. All rights reserved.

  9. Conversação eletrônica em trabalhos de arte e tecnologia

    Directory of Open Access Journals (Sweden)

    Fábio Oliveira Nunes

    2013-12-01

    Full Text Available http://dx.doi.org/10.5007/1807-9288.2013v9n2p76 Este artigo propõe uma reflexão em torno das dinâmicas de conversação eletrônica presentes em trabalhos de arte e tecnologia. Estas produções possuem sistemas que respondem a sentenças de seus interlocutores com proposições inteligíveis, dentro da ideia de uma linguagem natural, imitando uma conversa entre humanos. Essa conversação pode ser por texto ou mesmo por voz, quando o sistema possui recursos de reconhecimento de fala. Quando fruídos estes trabalhos revelam um interessante fenômeno, chamado de “Efeito Eliza”: a tendência humana de antropomorfizar os dispositivos tecnológicos, lendo comportamentos e respostas como resultados de uma “emoção humana” ainda que estes sejam apenas resultados aleatórios, repetitivos ou mesmo vazios de sentido. A denominação remete ao programa Eliza, apresentado em 1966 pelo cientista da computação alemão Joseph Weizenbaum nos Estados Unidos, que viria a ser um dos programas de Inteligência Artificial mais conhecidos do mundo. Do ponto de vista técnico, alguns destes trabalhos se aproximam muito da engenharia dos chatbots – robôs de conversação – que atualmente são bastante comuns na rede Internet para fins educacionais, comerciais ou mesmo, de entretenimento, como um “amigo virtual”. Neste contexto, é apresentada uma suposta teleperformance, capitaneada por um “artista tecnológico” chamado Mimo Steim. Assim como outras incursões do gênero, a ação envolve o potencial relacional de sistemas de conversação eletrônica em trabalhos de arte e tecnologia.

  10. Princípios de aprendizagem de jogos eletrônicos: gameficando a aula de línguas

    Directory of Open Access Journals (Sweden)

    Claudia Rodrigues Murta

    2015-04-01

    Full Text Available Resumo O objetivo deste artigo é discutir a aprendizagem propiciada pela experiência com jogos eletrônicos e como professores e escolas poderiam aprender com os jogos que apresentam princípios epistêmicos (SHAFFER et al., 2004. E ainda, apresentar a análise, segundo os princípios de Gee (2005; 2008, de nossa experiência em jogar um jogo comercial. Agameficação(DETERDING, 2011 é um processo que se vem disseminando como estratégia de aprendizagem. Esperamos com esta discussão despertar o interesse de professores para o potencial dos jogos eletrônicos na aprendizagem de línguas e demais conteúdos. Palavras-chave: Jogos eletrônicos.Aprendizagem.Gameficação.   Abstract The aim of this article is to discuss learning afforded by the experience of playing video games and how teachers and schools could learn from games that have epistemic principles (SHAFFER et al., 2004. We also present our experience in a commercial game, according to the principles of Gee (2005; 2008. The gamification (DETERDING, 2011 is a process that has been growing as a learning strategy. We hope that this discussion can draw teacher’s attention to the potential of computer games for language learning and other contents. Keywords: Video games.Learning.Gamification.

  11. A uniformização do Direito Internacional de compra e venda e os contratos eletrônicos

    Directory of Open Access Journals (Sweden)

    Guilherme Vitor de Gonzaga Camilo

    2016-06-01

    Full Text Available O presente trabalho visa investigar a relação entre a uniformização do Direito Internacional de Compra e Venda e as novas tecnologias, destacando-se aplicação da Convenção das Nações Unidas sobre Contratos de Compra e Venda Internacional de Mercadorias (CISG, instrumento que entrou em vigor no Brasil em 2014, em conjunto com a Convenção das Nações Unidas sobre o Uso das Comunicações Eletrônicas em Contratos Internacionais (CUECIC, instrumento que ainda não foi aderido pelo Brasil, mas já se encontra em vigor internacionalmente. Será também realizada uma analise crítica da convenção sobre o uso de comunicações eletrônicas, ressaltando-se outros meios de normatização da matéria de contratos eletrônicos internacionais, e perquirindo assim sobre a necessidade de adesão da CUECIC pelo Brasil.

  12. Comércio Eletrônico: A revolução em tempos digitais

    Directory of Open Access Journals (Sweden)

    Márcio Shoiti Kuniyoshi

    2009-01-01

    Full Text Available A Revolução Digital está gerando transformações significativas na Economia, trabalho e, principalmente, na forma de realizar negócios. Este artigo tem o objetivo de apresentar o panorama do comércio eletrônico, as tendências e os desafios proporcionados por essa ferramenta. A complexidade das transformações geradas pelo comércio eletrônico desperta interesse de pesquisa e entendimento de seus impactos, forma de superação de crise e criação de vantagem competitiva. Palavras-chave: tecnologia digital, Internet, estratégias de negócio, comércio eletrônico, competitividade, globalização. Abstract: The Digital Revolution is transforming the Economy, jobs and the way to realize business in the world. This article presents the Internet as a tool of business, Ecommerce environment; trends and the challenges to the organization that intend to use this new tool. The interest to research Internet and E-commerce is because they are the main sponsors of changes and creation of competitive advantage. Key words: digital technology, internet, business strategies, ecommerce, competition, globalization.

  13. Flavanol-rich cocoa consumption enhances exercise-induced executive function improvements in humans.

    Science.gov (United States)

    Tsukamoto, Hayato; Suga, Tadashi; Ishibashi, Aya; Takenaka, Saki; Tanaka, Daichi; Hirano, Yoshitaka; Hamaoka, Takafumi; Goto, Kazushige; Ebi, Kumiko; Isaka, Tadao; Hashimoto, Takeshi

    2018-02-01

    Aerobic exercise is known to acutely improve cognitive functions, such as executive function (EF) and memory function (MF). Additionally, consumption of flavanol-rich cocoa has been reported to acutely improve cognitive function. The aim of this study was to determine whether high cocoa flavanol (CF; HCF) consumption would enhance exercise-induced improvement in cognitive function. To test this hypothesis, we examined the combined effects of HCF consumption and moderate-intensity exercise on EF and MF during postexercise recovery. Ten healthy young men received either an HCF (563 mg of CF) or energy-matched low CF (LCF; 38 mg of CF) beverage 70 min before exercise in a single-blind counterbalanced manner. The men then performed moderate-intensity cycling exercise at 60% of peak oxygen uptake for 30 min. The participants performed a color-word Stroop task and face-name matching task to evaluate EF and MF, respectively, during six time periods throughout the experimental session. EF significantly improved immediately after exercise compared with before exercise in both conditions. However, EF was higher after HCF consumption than after LCF consumption during all time periods because HCF consumption improved EF before exercise. In contrast, HCF consumption and moderate-intensity exercise did not improve MF throughout the experiment. The present findings demonstrated that HCF consumption before moderate-intensity exercise could enhance exercise-induced improvement in EF, but not in MF. Therefore, we suggest that the combination of HCF consumption and aerobic exercise may be beneficial for improving EF. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Roles of Protein Synthesis Elongation Factor EF-Tu in Heat Tolerance in Plants

    Directory of Open Access Journals (Sweden)

    Jianming Fu

    2012-01-01

    Full Text Available EF-Tu proteins of plastids, mitochondria, and the cytosolic counterpart EF-1α in plants, as well as EF-Tu proteins of bacteria, are highly conserved and multifunctional. The functions of EF-Tu include transporting the aminoacyl-tRNA complex to the A site of the ribosome during protein biosynthesis; chaperone activity in protecting other proteins from aggregation caused by environmental stresses, facilitating renaturation of proteins when conditions return to normal; displaying a protein disulfide isomerase activity; participating in the degradation of N-terminally blocked proteins by the proteasome; eliciting innate immunity and triggering resistance to pathogenic bacteria in plants; participating in transcription when an E. coli host is infected with phages. EF-Tu genes are upregulated by abiotic stresses in plants, and EF-Tu plays important role in stress responses. Expression of a plant EF-Tu gene confers heat tolerance in E. coli, maize knock-out EF-Tu null mutants are heat susceptible, and over-expression of an EF-Tu gene improves heat tolerance in crop plants. This review paper summarizes the current knowledge of EF-Tu proteins in stress responses in plants and progress on application of EF-Tu for developing crop varieties tolerant to abiotic stresses, such as high temperatures.

  15. Sordarin derivatives induce a novel conformation of the yeast ribosome translocation factor eEF2

    DEFF Research Database (Denmark)

    Søe, Rikke; Mosley, R.T.; Justice, M.

    2007-01-01

    EF2 that form the ligand binding pocket are oriented in a different manner relative to the rest of eEF2 compared to our previous structure of eEF2 in complex with the parent natural product sordarin. Yeast eEF2 is also shown to bind adenylic nucleotides, which can be displaced by sordarin, suggesting...... that ADP or ATP also bind to the three C-terminal domains of eEF2. Fusidic acid is a universal inhibitor of translation that targets EF-G or eEF2, and is widely used as an antibiotic against gram positive bacteria. Based on mutations conferring resistance to fusidic acid, cryo-EM reconstructions, and X...

  16. [The EFS metrology: From the production to the reason].

    Science.gov (United States)

    Reifenberg, J-M; Riout, E; Leroy, A; Begue, S

    2014-06-01

    In order to answer statutory requirements and to anticipate the future needs and standards, the EFS is committed, since a few years, in a process of harmonization of its metrology function. In particular, the institution has opted for the skills development by internalizing the metrological traceability of the main critical quantities (temperature, volumetric) measurements. The development of metrology so resulted in a significant increase in calibration and testing activities. Methods are homogenized and improved through accreditations. The investment strategies are based on more and more demanding specifications. The performance of the equipments is better known and mastered. Technical expertise and maturity of the national metrology function today are assets to review in more informed ways the appropriateness of the applied periodicities. Analysis of numerous information and data in the calibration and testing reports could be pooled and operated on behalf of the unique establishment. The objective of this article is to illustrate these reflections with a few examples from of a feedback of the EFS Pyrénées Méditerranée. The analysis of some methods of qualification, the exploitation of the historical metrology in order to quantify the risk of non-compliance, and to adapt the control strategy, analysis of the criticality of an instrument in a measurement process, risk analyses are tools that deserve to be more widely exploited for that discipline wins in efficiency at the national level. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  17. Fabrication and tests of EF conductors for JT-60SA

    Energy Technology Data Exchange (ETDEWEB)

    Kizu, Kaname, E-mail: kizu.kaname@jaea.go.jp [Japan Atomic Energy Agency, Naka, Ibaraki 311-0193 (Japan); Kashiwa, Yoshitoshi; Murakami, Haruyuki [Japan Atomic Energy Agency, Naka, Ibaraki 311-0193 (Japan); Obana, Tetsuhiro; Takahata, Kazuya [National Institute for Fusion Science, Toki, Gifu 509-5292 (Japan); Tsuchiya, Katsuhiko; Yoshida, Kiyoshi [Japan Atomic Energy Agency, Naka, Ibaraki 311-0193 (Japan); Hamaguchi, Shinji [National Institute for Fusion Science, Toki, Gifu 509-5292 (Japan); Matsui, Kunihiro [Japan Atomic Energy Agency, Naka, Ibaraki 311-0193 (Japan); Nakamura, Kazuya; Takao, Tomoaki [Sophia University, Tokyo 102-8554 (Japan); Yanagi, Nagato; Imagawa, Shinsaku; Mito, Toshiyuki [National Institute for Fusion Science, Toki, Gifu 509-5292 (Japan)

    2011-10-15

    The conductors for plasma equilibrium field (EF) coils of JT-60SA are NbTi cable-in-conduit (CIC) conductor with stainless steel 316L jacket. The production of superconductors for actual EF coils started from February 2010. Nine superconductors with 444 m in length were produced up to July 2010. More than 300 welding of jackets were performed. Six nonconformities were found by inspections as go gauge, visual inspection and X-ray test. In order to shorten the manufacturing time schedule, helium leak test was conducted at once after connecting the long length jacket not just after the welding. The maximum force to pull the cable into jacket was about 7.6 kN on average. The mass flow rates of 9 conductors showed almost same values indicating that there are no blockages in the conductors. The measured current sharing temperature agreed with the expectation values from strand performance indicating that no degradation was caused by production process. The coupling time constants of conductors ranged from 80 to 90 ms which are much smaller than the design value of 200 ms.

  18. Clinical efficacy of gamma knife and surgery treatment of mesial temporal lobe epilepsy and their effects on EF-Tumt and EF-Tsmt expression.

    Science.gov (United States)

    Wang, X-Q; Zhang, X-D; Han, Y-M; Shi, X-F; Lan, Z-B; Men, X-X; Pan, Y-W

    2017-04-01

    To study the clinical efficacy of gamma knife and surgery treatment of mesial temporal lobe epilepsy (MTLE) and their effects on EF-Tumt and EF-Tsmt expression. The data of 78 cases of MTLE patients treated in our hospital from April 2011 to March 2013 were retrospectively analyzed. The patients were divided into two groups according to the treatment methods: the surgery group (including 41 cases) and the gamma knife group (including 37 cases). The clinical efficacy, the occurrence and recurrence of complications were evaluated, respectively; meanwhile, the expression of the EF-Tumt protein and EF-Tsmt protein in brain tissue were analyzed. The difference between the efficacy rate of the two groups showed no statistical significance (χ2=0.960, p>0.05). The complication rate of the gamma knife group was significantly lower than that of the control group (χ2=6.430, pknife group was significantly lower than that of the patients in the surgery group (p>0.05). Within the two groups, the positive expression granum of EF-Tsmt protein and EF-Tumt protein of the two groups after treatment were significantly lower than that before treatment (pknife group was obviously more than that of the patients in the surgery group (p0.05). Before and after treatment within the group, the positive cell of EF-Tsmt protein and EF-Tumt protein of the two groups of patients after treatment were significantly lower than that before treatment (p0.05). Both surgery and gamma knife could treat MTLE effectively, and the efficacy may be related to the ability to reduce the expression of EF-Tsmt protein and EF-Tumt protein in brain tissue.

  19. Tabagismo, cigarros eletrônicos e redução de danos: uma revisão narrativa / Smoking, electronic cigarettes and harm reduction: a narrative review

    Directory of Open Access Journals (Sweden)

    Ivan Farias Barreto

    2018-03-01

    Full Text Available O tabagismo é um grave problema de saúde pública no Brasil. O presente trabalho investigou o uso dos cigarros eletrônicos como possibilidade para reduzir os danos do consumo de tabaco. Realizou-se uma revisão narrativa de literatura, privilegiando artigos em revistas científicas, livros e outras fontes relevantes. Os resultados indicam que dispositivos eletrônicos para fumar podem ser menos prejudiciais à saúde se comparados a cigarros combustíveis e, portanto, têm potencial de serem utilizados para reduzir os danos do consumo de tabaco. Por outro lado, podem levar populações mais vulneráveis à iniciação ao tabagismo e ainda requerem estudos a fim de avaliar os efeitos na saúde a longo prazo. Da mesma forma que os cigarros combustíveis, conclui-se que cigarros eletrônicos, se regulamentados, devem ser submetidos a programas de controles austeros, incluindo, por exemplo, políticas tributárias, de proteção a não fumantes e restrição de publicidade. Smoking is a serious Brazilian public health problem. This work investigated the electronic cigarettes as a possibility to reduce damages of tobacco consumption. The used methodology was the narrative review of the literature, privileging articles in scientific journals, books, and other relevant sources. The results indicate that electronic smoking devices may be less harmful to health if compared to combustible cigarettes and have potential to reduce the harm of tobacco use. On the other hand, such devices may lead vulnerable populations to smoking initiation and still require studies to access their effects on human health in the long term. In the same way as combustible cigarettes, it is concluded that electronic cigarettes, if regulated, should be subjected to austere control programs, including, for example, tax policies, non-smoking protection, and advertising restriction.

  20. The eEF1A proteins: at the crossroads of oncogenesis, apoptosis and viral infections.

    Directory of Open Access Journals (Sweden)

    Georges eHerbein

    2015-04-01

    Full Text Available Eukaryotic translation elongation factors 1 alpha, eEF1A1 and eEF1A2, are not only translation factors, but also pleiotropic proteins that are highly expressed in human tumors, including breast cancer, ovarian cancer and lung cancer. eEF1A1 modulates cytoskeleton, exhibits chaperone-like activity and also controls cell proliferation and cell death. By contrast eEF1A2 protein favors oncogenesis as shown by the fact that overexpression of eEF1A2 leads to cellular transformation and gives rise to tumors in nude mice. The eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. By contrast, inactivation of eEF1A proteins leads to immunodeficiency, neural and muscular defects, and favors apoptosis. Finally, eEF1A proteins interact with several viral proteins resulting in enhanced viral replication, decreased apoptosis and increased cellular transformation. This review summarizes the recent findings on eEF1A proteins indicating that eEF1A proteins play a critical role in numerous human diseases through enhancement of oncogenesis, blockade of apoptosis and increased viral pathogenesis.

  1. Engineering recombinant EF-hand peptides for selective uranium uptake

    Energy Technology Data Exchange (ETDEWEB)

    Berthomieu, C.; Pardoux, R.; Beccia, M.R.; Lemaire, D.; Sauge-Merle, S. [CEA, DSV, IBEB, UMR7265 CNRS CEA Aix-Marseille Univ. (France); Guilbaud, P. [CEA, DRCPC, SMCS, LILA (France); Delangle, P. [CEA, INAC, Service de Chimie Inorganique et Biologique (France)

    2014-07-01

    In spite of an increasing number of publications in recent years, information regarding the mechanism of uranium interaction with proteins at the molecular level is limited and few quantitative studies have investigated the binding properties of uranyl with proteins or peptides. It is thus of great interest to better characterize these interactions, and to analyze structural factors governing uranyl binding and thermodynamic stabilization in proteins. Research in this direction will benefit our understanding of the molecular factors governing uranyl toxicity and speciation in cells and will also aid in developing new molecules for selectively binding uranium that could be used for uranium bioremediation purposes. Uranyl coordination properties have similarities with those of calcium, i.e electrostatic interactions preferentially with hard donor oxygen ligands and pentagonal bipyramidal structures. The EF-hand structural motif is the most prevalent Ca2{sup +-}binding site in proteins and is very appealing to analyse uranyl binding properties and develop affine and specific uranyl binding sites for biotechnology approaches. We selected the recombinant N-terminal domain of calmodulin from A. thaliana as a structured template that contains two EF-hand motifs (site I and site II) to analyze its uranyl binding properties and to engineer peptide variants with increased uranyl affinity and specificity. We showed that both site I and site II bind uranyl, with a dissociation constant in the nano-molar range for site I (K{sub d} ≅ 25 nM at pH 6). Using in vitro phosphorylation of a threonine located in the uranyl binding loop, we measured how adding a phosphoryl group affects the calcium and uranium binding affinities. The phosphorylated peptide exhibited a very large affinity for uranyl at pH 7, with a dissociation constant in the sub-nano-molar range K{sub d} = 0.25 ±0.06 nM, and FTIR analysis demonstrated that the phosphoryl group plays a determining role in uranyl

  2. Discovery of optical flickering from the symbiotic star EF Aquilae

    Science.gov (United States)

    Zamanov, R. K.; Boeva, S.; Nikolov, Y. M.; Petrov, B.; Bachev, R.; Latev, G. Y.; Popov, V. A.; Stoyanov, K. A.; Bode, M. F.; Martí, J.; Tomov, T.; Antonova, A.

    2017-07-01

    We report optical CCD photometry of the recently identified symbiotic star EF Aql. Our observations in Johnson V and B bands clearly show the presence of stochastic light variations with an amplitude of about 0.2 mag on a time scale of minutes. The observations point toward a white dwarf (WD) as the hot component in the system. It is the 11-th object among more than 200 symbiotic stars known with detected optical flickering. Estimates of the mass accretion rate onto the WD and the mass loss rate in the wind of the Mira secondary star lead to the conclusion that less than 1 per cent of the wind is captured by the WD. Eight further candidates for the detection of flickering in similar systems are suggested.

  3. A comparison of HFrEF vs HFpEF's clinical workload and cost in the first year following hospitalization and enrollment in a disease management program.

    Science.gov (United States)

    Murphy, T M; Waterhouse, D F; James, S; Casey, C; Fitzgerald, E; O'Connell, E; Watson, C; Gallagher, J; Ledwidge, M; McDonald, K

    2017-04-01

    Admission with heart failure (HF) is a milestone in the progression of the disease, often resulting in higher intensity medical care and ensuing readmissions. Whilst there is evidence supporting enrolling patients in a heart failure disease management program (HF-DMP), not all reported HF-DMPs have systematically enrolled patients with HF with preserved ejection fraction (HFpEF) and there is a scarcity of literature differentiating costs based on HF-phenotype. 1292 consenting, consecutive patients admitted with a primary diagnosis of HF were enrolled in a hospital based HF-DMP and categorized as HFpEF (EF≥45%) or HFrEF (EFHospitalizations, primary care, medications, and DMP workload with associated costs were evaluated assessing DMP clinic-visits, telephonic contact, medication changes over 1year using a mixture of casemix and micro-costing techniques. The total average annual cost per patient was marginally higher in patients with HFrEF €13,011 (12,011, 14,078) than HFpEF, €12,206 (11,009, 13,518). However, emergency non-cardiovascular admission rates and average cost per patient were higher in the HFpEF vs HFrEF group (0.46 vs 0.31 per patient/12months) & €655 (318, 1073) vs €584 (396, 812). In the first 3months of the outpatient HF-DMP the HFrEF population cost more on average €791 (764, 819) vs €693 (660, 728). There are greater short-term (3-month) costs of HFrEF versus HFpEF as part of a HF-DMP following an admission. However, long-term (3-12month) costs of HFpEF are greater because of higher non-cardiovascular rehospitalisations. As HFpEF becomes the dominant form of HF, more work is required in HF-DMPs to address prevention of non-cardiovascular rehospitalisations and to integrate hospital based HF-DMPs into primary healthcare structures. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

  4. O uso do comércio eletrônico no ramo de flores tropicais em Pernambuco.

    OpenAIRE

    Euri Charles Andrade da Silva

    2010-01-01

    Comércio eletrônico (ou e-commerce) refere-se ao uso da Internet para realizar negócios. Mais formalmente, diz respeito às transações comerciais realizadas digitalmente entre organizações e indivíduos ou entre duas ou mais organizações. O estudo de caso desenvolvido nessa pesquisa permitiu o conhecimento da cadeia produtiva de flores tropicais em Pernambuco, que se destaca ocupando o quinto lugar no País na produção e comercialização do setor. A pesquisa é realizada em quatro empresas que neg...

  5. Proposta de um modelo de referência para governo eletrônico 2.0

    OpenAIRE

    Dziekaniak, Gisele Vasconcelos

    2011-01-01

    Propõe-se um modelo de referência para governo eletrônico (e-gov) fundamentado nas prerrogativas da Web 2.0. Este modelo visa contribuir para o desenvolvimento de governos 2.0, os quais são baseados no compartilhamento do conhecimento, bem como na gestão e criação de conteúdos por coletivos e no fortalecimento das relações governo e sociedade. Utiliza-se a pesquisa bibliográfica, assim como a observação direta de redes sociais que possuem como objeto de discussão as plataformas governam...

  6. Um ambiente virtual para o planejamento de capacidade de ambientes voltados ao comércio eletrônico

    OpenAIRE

    Costa, Eduardo Dockhron da

    2005-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Ciência da Computação. Um dos grandes desafios existentes no comércio eletrônico é a correta implementação de sua infra-estrutura. Os componentes de hardware e software estão, com o passar do tempo, sofrendo um grande incremento de capacidade e velocidade de processamento. Aumenta-se, em paralelo, o custo para se obter tecnologia de ponta. Nessa escala de crescimento, os nív...

  7. Pathophysiological understanding of HFpEF: microRNAs as part of the puzzle.

    Science.gov (United States)

    Rech, Monika; Barandiarán Aizpurua, Arantxa; van Empel, Vanessa; van Bilsen, Marc; Schroen, Blanche

    2018-05-01

    Half of all heart failure patients have preserved ejection fraction (HFpEF). Comorbidities associated with and contributing to HFpEF include obesity, diabetes and hypertension. Still, the underlying pathophysiological mechanisms of HFpEF are unknown. A preliminary consensus proposes that the multi-morbidity triggers a state of systemic, chronic low-grade inflammation, and microvascular dysfunction, causing reduced nitric oxide bioavailability to adjacent cardiomyocytes. As a result, the cardiomyocyte remodels its contractile elements and fails to relax properly, causing diastolic dysfunction, and eventually HFpEF. HFpEF is a complex syndrome for which currently no efficient therapies exist. This is notably due to the current one-size-fits-all therapy approach that ignores individual patient differences. MicroRNAs have been studied in relation to pathophysiological mechanisms and comorbidities underlying and contributing to HFpEF. As regulators of gene expression, microRNAs may contribute to the pathophysiology of HFpEF. In addition, secreted circulating microRNAs are potential biomarkers and as such, they could help stratify the HFpEF population and open new ways for individualized therapies. In this review, we provide an overview of the ever-expanding world of non-coding RNAs and their contribution to the molecular mechanisms underlying HFpEF. We propose prospects for microRNAs in stratifying the HFpEF population. MicroRNAs add a new level of complexity to the regulatory network controlling cardiac function and hence the understanding of gene regulation becomes a fundamental piece in solving the HFpEF puzzle.

  8. O texto eletrônico hipertextualizado: a máquina, o autor e o leitor

    Directory of Open Access Journals (Sweden)

    Emanoel Cesar Pires de Assis

    2012-09-01

    Full Text Available http://dx.doi.org/10.5007/2176-8552.2011nesp1p110 O leitor de uma narrativa tradicional está temporalmente marcado por uma estrutura de começo, meio e fim. Já o leitor interativo-imersivo da narrativa eletrônica experimenta temporalidades narrativas múltiplas; diríamos mais, além de múltiplas elas podem ser análogas e/ou contraditórias, uma vez que a estrutura descentralizada da leitura hipertextual desloca espaço e tempo, dando ao leitor a experiência de uma leitura topográfica; ou seja, visual e estruturalmente concebida como se fosse a leitura de um mapa, onde não há um ponto inicial estabelecido, mas, sim, vários caminhos que podem ser seguidos. Ao deslocar o tempo, a leitura hipertextual torna-se um presente constante e contínuo. Assim: “O leitor eletrônico lê e não conclui sua leitura no tempo. O que faz é experimentar constantemente, em releituras sucessivas, possibilidades narrativas alternativas em uma vasta dimensão espacial e em um eterno presente” (BELLEI, 2002, p. 122.

  9. Discussão Ética sobre o Prontuário Eletrônico do Paciente

    Directory of Open Access Journals (Sweden)

    Maria José Guedes Gondim Almeida

    Full Text Available RESUMO O prontuário do paciente constitui uma ferramenta fundamental para a prestação do cuidado em saúde, podendo ser definido como um registro padronizado e organizado de toda a informação referente à saúde de um indivíduo, desde o nascimento até a morte. Na década de 1970, impulsionados pelo crescente desenvolvimento da tecnologia, surgem os primeiros sistemas de Prontuários Eletrônicos do Paciente (PEP, sob uma proposta de informatização dos registros em saúde. Por meio de uma criteriosa pesquisa bibliográfica, questões concernentes ao prontuário eletrônico do paciente foram discutidas, especialmente do ponto de vista ético. Os avanços tecnológicos aplicados à área de saúde devem ser implantados de maneira crítica, em que pesem as consequências imprevistas que possam ter quanto à relação médico-paciente, uma vez que o cuidado e o respeito devem ser prioridade, e não os interesses particulares. Por isso, uma análise apenas técnica não abarca elementos que podem ser compreendidos à luz dos conceitos éticos, os quais norteiam escolhas baseadas no interesse comum.

  10. Mensuração da qualidade de serviço no varejo eletrônico e seu impacto sobre as intenções comportamentais

    OpenAIRE

    Vieira, Valter Afonso

    2010-01-01

    O construto qualidade de serviços tem sido amplamente pesquisado na literatura de Marketing em termos de antecedentes, consequentes, dimensões, e mensurações. No entanto, há uma carência na realização não somente de pesquisas sobre a qualidade dos serviços em varejo eletrônico, como também sobre a definição e a dimensionalidade do construto nesse ambiente. Sob esse pretexto, o trabalho buscou compreender mais a fundo o construto qualidade percebida no varejo eletrônico. Especificamente, este ...

  11. 75 FR 41894 - Wapakoneta Machine Company, Currently Known as EF Industrial Technologies, Inc., Wapakoneta, OH...

    Science.gov (United States)

    2010-07-19

    ... of early 2010, Wapakoneta Machine Company is currently known as EF Industrial Technologies, Inc. Some... Wapakoneta Machine Company, currently known as EF Industrial Technologies, Inc., Wapakoneta, Ohio became... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-73,211] Wapakoneta Machine...

  12. Mapping the human translation elongation factor eEF1H complex using the yeast two-hybrid system

    DEFF Research Database (Denmark)

    Mansilla, Francisco; Friis, Irene; Jadidi, Mandana

    2002-01-01

    In eukaryotes, the eukaryotic translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. In metazoans, eEF1B consists of three subunits: eEF1B alpha, eEF1B eta and eEF1B...... of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex. However, these models differ in various aspects. This might be due to the difficulties of handling, particularly the eEF1B beta and eEF1B gamma subunits in vitro. Here, the human eEF1H complex is for the first...... gamma:eEF1B beta, where the last was observed using a three-hybrid approach. Surprisingly, eEF1A2 showed no or only little affinity for the guanine-nucleotide-exchange factors. Truncated versions of the subunits of eEF1B were used to orientate these subunits within the resulting model. The model unit...

  13. Complex distribution of EFL and EF-1α proteins in the green algal lineage

    Directory of Open Access Journals (Sweden)

    Keeling Patrick J

    2007-05-01

    Full Text Available Abstract Background EFL (or elongation factor-like is a member of the translation superfamily of GTPase proteins. It is restricted to eukaryotes, where it is found in a punctate distribution that is almost mutually exclusive with elongation factor-1 alpha (EF-1α. EF-1α is a core translation factor previously thought to be essential in eukaryotes, so its relationship to EFL has prompted the suggestion that EFL has spread by horizontal or lateral gene transfer (HGT or LGT and replaced EF-1α multiple times. Among green algae, trebouxiophyceans and chlorophyceans have EFL, but the ulvophycean Acetabularia and the sister group to green algae, land plants, have EF-1α. This distribution singles out green algae as a particularly promising group to understand the origin of EFL and the effects of its presence on EF-1α. Results We have sampled all major lineages of green algae for both EFL and EF-1α. EFL is unexpectedly broad in its distribution, being found in all green algal lineages (chlorophyceans, trebouxiophyceans, ulvophyceans, prasinophyceans, and mesostigmatophyceans, except charophyceans and the genus Acetabularia. The presence of EFL in the genus Mesostigma and EF-1α in Acetabularia are of particular interest, since the opposite is true of all their closest relatives. The phylogeny of EFL is poorly resolved, but the Acetabularia EF-1α is clearly related to homologues from land plants and charophyceans, demonstrating that EF-1α was present in the common ancestor of the green lineage. Conclusion The distribution of EFL and EF-1α in the green lineage is not consistent with the phylogeny of the organisms, indicating a complex history of both genes. Overall, we suggest that after the introduction of EFL (in the ancestor of green algae or earlier, both genes co-existed in green algal genomes for some time before one or the other was lost on multiple occasions.

  14. Neuroimagem na dependência de jogos eletrônicos: uma revisão sistemática

    Directory of Open Access Journals (Sweden)

    Igor Lins Lemos

    2014-03-01

    Full Text Available Objetivo: Realizar revisão sistemática de manuscritos que utilizaram a neuroimagem no estudo da dependência de jogos eletrônicos, a fim de identificar as principais regiões cerebrais alteradas. Métodos: Foram realizadas buscas nos seguintes bancos de dados: ScieELO, BVS, Lilacs, Science Direct On Line e PubMed. Não houve data mínima para a pesquisa, sendo considerados os artigos encontrados até julho de 2013. Os descritores utilizados para a presente revisão sistemática da literatura foram: “PET”, “SPECT”, “MRI”, “DTI”, “EEG”, “imaging”, “neuroimaging”, “spectroscopy”, “functional magnetic ressonance”, “structural magnetic ressonance”, “tractography”, “voxel” e “brain”, individualmente cruzados com os descritores “gaming” e “video game addiction”. Resultados: Dos 52 artigos encontrados, 16 foram selecionados: nove usaram fMRI, quatro usaram sRMI, um usou PET e dois usaram EEG. Em relação às alterações funcionais e estruturais, elas foram mais observadas no lobo frontal (córtex pré-frontal dorsolateral, córtex orbitofrontal, giro pré-frontal, giro frontal médio, parietal, temporal (giro para-hipocampal, núcleos da base, tálamo, ínsula e cerebelo. Conclusão: A despeito dos métodos utilizados, os estudos apontaram convergências quanto às reciprocidades cerebrais. Essas alterações neurais são semelhantes às observadas em pacientes dependentes de substâncias e de internet, especialmente durante o estado de fissura. Apesar de apenas recentemente pesquisas de neuroimagem em dependentes de jogos eletrônicos terem sido realizadas, contamos no momento com achados significativos alinhados à compreensão dos mecanismos neurais associados à dependência de jogos eletrônicos e respectiva inserção como categoria nosológica no âmbito psiquiátrico.

  15. In vivo and in vitro suppression of hepatocellular carcinoma by EF24, a curcumin analog.

    Directory of Open Access Journals (Sweden)

    Haitao Liu

    Full Text Available The synthetic compound 3,5-bis(2-flurobenzylidenepiperidin-4-one (EF24 is a potent analog of curcumin that exhibits enhanced biological activity and bioavailability without increasing toxicity. EF24 exerts antitumor activity by arresting the cell cycle and inducing apoptosis, suppressing many types of cancer cells in vitro. The antiproliferative and antiangiogenic properties of EF24 provide theoretical support for its development and application to liver cancers. We investigated the in vitro and in vivo activities of EF24 on liver cancer to better understand its therapeutic effects and mechanisms. EF24 induced significant apoptosis and G2/M-phase cell cycle arrest in mouse liver cancer cell lines, Hepa1-6 and H22. The expression levels of G2/M cell cycle regulating factors, cyclin B1 and Cdc2, were significantly decreased, pp53, p53, and p21 were significantly increased in EF24-treated cells. In addition, EF24 treatment significantly reduced Bcl-2 concomitant with an increase in Bax, enhanced the release of cytochrome c from the mitochondria into the cytosol, resulting in an upregulation of cleaved-caspase-3, which promoted poly (ADP-ribose polymerase cleavage. EF24-treated cells also displayed decreases in phosphorylated Akt, phosphorylated extracellular signal-regulated kinase and vascular endothelial growth factor. Our in vitro protein expression data were confirmed in vivo using a subcutaneous hepatocellular carcinoma (HCC tumor model. This mouse HCC model confirmed that total body weight was unchanged following EF24 treatment, although tumor weight was significantly decreased. Using an orthotopic HCC model, EF24 significantly reduced the liver/body weight ratio and relative tumor areas compared to the control group. In situ detection of apoptotic cells and quantification of Ki-67, a biomarker of cell proliferation, all indicated significant tumor suppression with EF24 treatment. These results suggest that EF24 exhibits anti-tumor activity

  16. Sustainable Consumption

    DEFF Research Database (Denmark)

    Røpke, Inge

    2015-01-01

    The intention of this chapter is to explore the role of consumption and consumers in relation to sustainability transition processes and wider systemic transformations. In contrast to the individualistic focus in much research on sustainable consumption, the embeddedness of consumption activities...... in wider social, economic and technological frameworks is emphasised. In particular, the chapter is inspired by practice theory and transition theory. First, various trends in consumption are outlined to highlight some of the challenges for sustainability transitions. Then, it is discussed how consumption...... patterns are shaped over time and what should be considered in sustainability strategies. While discussions on consumption often take their point of departure in the perspective of the individual and then zoom to the wider context, the present approach is the opposite. The outline starts with the basic...

  17. Desenvolvimento de um dispositivo eletrônico para organizar medicamentos e promover a adesão medicamentosa

    Directory of Open Access Journals (Sweden)

    Liliana Batista Vieira

    Full Text Available RESUMO O presente artigo descreve o desenvolvimento de um protótipo eletrônico para organizar medicamentos, denominado Sistema Eletrônico de Uso Personalizado e Controlado de Medicamentos (SUPERMED. O protótipo inclui uma gaveta que contém os medicamentos a serem utilizados durante 1 mês, alarme sonoro e visual programado para disparar nos horários de administração dos medicamentos e registro dos horários em que a caixa foi aberta (horário previsto ou fora do horário previsto gravado em cartão de memória e posteriormente transferido para leitura em computador. Para o desenvolvimento do protótipo, seguiu-se o modelo de prototipação evolucionária. O SUPERMED foi projetado sobre a plataforma Arduino e a programação foi desenvolvida em linguagem C. Para leitura do sistema de alertas e registro dos horários de abertura da caixa, desenvolveu-se um software em linguagem Java. Uma vez feita a programação dos alarmes (idealmente por um profissional da área de saúde, não há nenhum ajuste adicional a ser feito pelo paciente. As funcionalidades do protótipo foram testadas durante 31 dias pelos pesquisadores, com funcionamento bem-sucedido. O sistema parece adequado para organizar os medicamentos e facilitar a adesão medicamentosa. Novos estudos serão realizados para validar a aperfeiçoar o protótipo.

  18. Engajamento dos Consumidores com o Boca a Boca Eletrônico Negativo em Lojas de Aplicativos Móveis

    Directory of Open Access Journals (Sweden)

    Adrianne Paula Vieira de Andrade

    2017-11-01

    Full Text Available O objetivo do estudo é compreender as motivações do comportamento de engajamento do consumidor com o boca a boca eletrônico (electronic word-of–mouth [eWOM] negativo em plataformas de consumo de aplicativos móveis, utilizando-se como lente de análise a Teoria da Ação Racional (Theory of Reasoned Action [TRA]. Foram realizadas dez entrevistas em profundidade com usuários emissores de comentários negativos, as quais permitiram a construção de um corpus que foi descrito, classificado e interpretado por meio de análise temática de conteúdo. Como generalização analítica, infere-se que os motivadores mais influentes para o engajamento com o eWOM negativo são a Busca por Providências, o Altruísmo, as Emoções Negativas e a Reciprocidade. Com relação aos construtos da TRA, as Atitudes favorecem esse engajamento, mas as Normas subjetivas pouco contribuem para a participação ativa no boca a boca eletrônico negativo. Esta pesquisa pode ajudar os programadores de aplicativos móveis e os analistas de mídias sociais quanto à formulação de estratégias de interação que estimulem o comportamento de engajamento dos consumidores.

  19. As Experiências de Consumo de Eletrônicos de Consumidores de Baixa Renda no Brasil

    Directory of Open Access Journals (Sweden)

    Marcelo de Rezende Pinto

    2013-03-01

    Full Text Available A partir da constatação de que ainda são incipientes os estudos que buscam relacionar as experiências de consumo em uma perspectiva social, atrelada à percepção de que pouco se pesquisou sobre os consumidores pobres urbanos, este trabalho tem por objetivo geral investigar como as experiências de consumo de eletrônicos pelos consumidores de baixa renda interagem com o sistema cultural e simbólico envolvido no fluxo da vida social cotidiana. Por meio de uma abordagem interpretativista e construtivista, conduziu-se uma pesquisa de inspiração etnográfica a fim de se vivenciar o dia a dia dos consumidores no seu ambiente natural por meio de entrevistas em profundidade, observações e notas de campo. Os dados da investigação empírica foram examinados com base na Análise de Conteúdo. Com a intenção de ir além das descrições, o trabalho também visou à construção de uma teoria substantiva por meio da grounded theory construtivista tal como proposta por Strauss e Corbin (2008 e Charmaz (2006. A teoria substantiva gerada salientou que nas experiências de consumo de aparelhos eletrônicos de consumidores pobres é possível perceber uma intensa relação com o sistema cultural e simbólico construída entre os sujeitos pesquisados.DOI:10.5585/remark.v12i1.2316

  20. Regulation of eukaryotic elongation factor 1 alpha (eEF1A) by dynamic lysine methylation

    DEFF Research Database (Denmark)

    Jakobsson, Magnus E; Małecki, Jędrzej; Falnes, Pål Ø

    2018-01-01

    Lysine methylation is a frequent post-translational protein modification, which has been intensively studied in the case of histone proteins. Lysine methylations are also found on many non-histone proteins, and one prominent example is eukaryotic elongation factor 1 alpha (eEF1A). Besides its...... essential role in the protein synthesis machinery, a number of non-canonical functions have also been described for eEF1A, such as regulation of the actin cytoskeleton and the promotion of viral replication. The functional significance of the extensive lysine methylations on eEF1A, as well as the identity...

  1. Dual-Polarimetric Radar-Based Tornado Debris Paths Associated with EF-4 and EF-5 Tornadoes over Northern Alabama During the Historic Outbreak of 27 April 2011

    Science.gov (United States)

    Carey, Lawrence D.; Schultz, Chrstopher J.; Schultz, Elise V.; Petersen, Walter A.; Gatlin, Patrick N.; Knupp, Kevin R.; Molthan, Andrew L.; Jedlovec, Gary J.; Darden, Christopher B.

    2012-01-01

    An historic tornado and severe weather outbreak devastated much of the southeastern United States between 25 and 28 April 2011. On 27 April 2011, northern Alabama was particularly hard hit by a large number of tornadoes, including several that reached EF-4 and EF-5 on the Enhanced Fujita damage scale. In northern Alabama alone, there were approximately 100 fatalities and hundreds of more people who were injured or lost their homes during the havoc caused by these violent tornadic storms. Two long-track and violent (EF-4 and EF-5) tornadoes occurred within range of the University of Alabama in Huntsville (UAHuntsville) Advanced Radar for Meteorological and Operational Research (ARMOR, C-band dual-polarimetric). A unique capability of dual-polarimetric radar is the near-real time identification of lofted debris associated with ongoing tornadoes on the ground. The focus of this paper is to analyze the dual-polarimetric radar-inferred tornado debris signatures and identify the associated debris paths of the long-track EF-4 and EF-5 tornadoes near ARMOR. The relative locations of the debris and damage paths for each tornado will be ascertained by careful comparison of the ARMOR analysis with NASA MODIS (Moderate Resolution Imaging Spectroradiometer) and ASTER (Advanced Spaceborne Thermal Emission and Reflection Radiometer) satellite imagery of the tornado damage scenes and the National Weather Service tornado damage surveys. With the ongoing upgrade of the WSR-88D (Weather Surveillance Radar 1988 Doppler) operational network to dual-polarimetry and a similar process having already taken place or ongoing for many private sector radars, dual-polarimetric radar signatures of tornado debris promise the potential to assist in the situational awareness of government and private sector forecasters and emergency managers during tornadic events. As such, a companion abstract (Schultz et al.) also submitted to this conference explores The use of dual-polarimetric tornadic debris

  2. Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification

    Czech Academy of Sciences Publication Activity Database

    Jonák, Jiří

    2007-01-01

    Roč. 849, 1-2 (2007), s. 141-153 ISSN 1570-0232 R&D Projects: GA AV ČR IAA5052206; GA AV ČR KJB500520503; GA MŠk 2B06065 Institutional research plan: CEZ:AV0Z50520514 Keywords : bacterial elongation factors EF-Tu, , G-domain * recombinant EF-Tus * preparation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.935, year: 2007

  3. Heat-Killed Enterococcus faecalis EF-2001 Ameliorates Atopic Dermatitis in a Murine Model

    Science.gov (United States)

    Choi, Eun-Ju; Iwasa, Masahiro; Han, Kwon-Il; Kim, Wan-Jae; Tang, Yujiao; Hwang, Young Joung; Chae, Jeong Ryong; Han, Weon Cheol; Shin, Yu-Su; Kim, Eun-Kyung

    2016-01-01

    Recent reports have shown the immunomodulatory effect of heat-killed lactic acid bacteria. Atopic dermatitis (AD) is an allergic skin disease, caused by immune dysregulation among other factors. The aim of this study was to assess the effect of heat-killed Enterococcus faecalis EF-2001 (EF-2001) on AD. We established an in vivo AD model by repeated local exposure of Dermatophagoides farinae extract (DFE; house dust mite extract) and 2,4-dinitrochlorobenzene (DNCB) to the ears of mice. After oral administration of EF-2001 for four weeks, the epidermal and dermal ear thickness, mast cell infiltration, and serum immunoglobulin levels were measured. In addition, the gene expression levels of pathogenic cytokines in the ears, lymph nodes, and splenocytes were assayed. EF-2001 attenuated AD symptoms based on the ear thickness, histopathological analysis, and serum immunoglobulin levels. Moreover, EF-2001 decreased the DFE/DNCB-induced expression of various pathogenic cytokines in the ears, lymph nodes, and splenocytes. These results suggest that EF-2001 has therapeutic potential in the treatment of AD owing to its immunomodulatory effects. PMID:26959058

  4. Heat-Killed Enterococcus faecalis EF-2001 Ameliorates Atopic Dermatitis in a Murine Model

    Directory of Open Access Journals (Sweden)

    Eun-Ju Choi

    2016-03-01

    Full Text Available Recent reports have shown the immunomodulatory effect of heat-killed lactic acid bacteria. Atopic dermatitis (AD is an allergic skin disease, caused by immune dysregulation among other factors. The aim of this study was to assess the effect of heat-killed Enterococcus faecalis EF-2001 (EF-2001 on AD. We established an in vivo AD model by repeated local exposure of Dermatophagoides farinae extract (DFE; house dust mite extract and 2,4-dinitrochlorobenzene (DNCB to the ears of mice. After oral administration of EF-2001 for four weeks, the epidermal and dermal ear thickness, mast cell infiltration, and serum immunoglobulin levels were measured. In addition, the gene expression levels of pathogenic cytokines in the ears, lymph nodes, and splenocytes were assayed. EF-2001 attenuated AD symptoms based on the ear thickness, histopathological analysis, and serum immunoglobulin levels. Moreover, EF-2001 decreased the DFE/DNCB-induced expression of various pathogenic cytokines in the ears, lymph nodes, and splenocytes. These results suggest that EF-2001 has therapeutic potential in the treatment of AD owing to its immunomodulatory effects.

  5. delta-EF1 is a negative regulator of Ihh in the developing growth plate.

    Science.gov (United States)

    Bellon, Ellen; Luyten, Frank P; Tylzanowski, Przemko

    2009-11-30

    Indian hedgehog (Ihh) regulates proliferation and differentiation of chondrocytes in the growth plate. Although the biology of Ihh is currently well documented, its transcriptional regulation is poorly understood. delta-EF1 is a two-handed zinc finger/homeodomain transcriptional repressor. Targeted inactivation of mouse delta-EF1 leads to skeletal abnormalities including disorganized growth plates, shortening of long bones, and joint fusions, which are reminiscent of defects associated with deregulation of Ihh signaling. Here, we show that the absence of delta-EF1 results in delayed hypertrophic differentiation of chondrocytes and increased cell proliferation in the growth plate. Further, we demonstrate that delta-EF1 binds to the putative regulatory elements in intron 1 of Ihh in vitro and in vivo, resulting in down-regulation of Ihh expression. Finally, we show that delta-EF1 haploinsufficiency leads to a postnatal increase in trabecular bone mass associated with enhanced Ihh expression. In summary, we have identified delta-EF1 as an in vivo negative regulator of Ihh expression in the growth plate.

  6. Labeled EF-Tus for rapid kinetic studies of pretranslocation complex formation

    DEFF Research Database (Denmark)

    Liu, Wei; Kavaliauskas, Darius; Schrader, Jared

    2014-01-01

    The universally conserved translation elongation factor EF-Tu delivers aminoacyl(aa)-tRNA in the form of an aa-tRNA·EF-Tu·GTP ternary complex (TC) to the ribosome where it binds to the cognate mRNA codon within the ribosomal A-site, leading to formation of a pretranslocation (PRE) complex. Here we...... describe preparation of QSY9 and Cy5 derivatives of the variant E348C-EF-Tu that are functional in translation elongation. Together with fluorophore derivatives of aa-tRNA and of ribosomal protein L11, located within the GTPase associated center (GAC), these labeled EF-Tus allow development of two new FRET...... assays that permit the dynamics of distance changes between EF-Tu and both L11 (Tu-L11 assay) and aa-tRNA (Tu-tRNA assay) to be determined during the decoding process. We use these assays to examine: (i) the relative rates of EF-Tu movement away from the GAC and from aa-tRNA during decoding, (ii...

  7. Contribution of effluent organic matter (EfOM) to ultrafiltration (UF) membrane fouling: Isolation, characterization, and fouling effect of EfOM fractions

    KAUST Repository

    Zheng, Xing

    2014-11-01

    EfOM has been regarded as a major organic foulant resulting in UF membrane fouling in wastewater reclamation. To investigate fouling potential of different EfOM fractions, the present study isolated EfOM into hydrophobic neutrals (HPO-N), colloids, hydrophobic acids (HPO-A), transphilic neutrals and acids (TPI), and hydrophilics (HPI), and tested their fouling effect in both salt solution and pure water during ultrafiltration (UF). Major functional groups and chemical structure of the isolates were identified using Fourier transform infrared spectroscopy (FT-IR) and solid-state carbon nuclear magnetic resonance (13C NMR) analysis. The influence of the isolation process on the properties of EfOM fractions was minor because the raw and reconstituted secondary effluents were found similar with respect to UV absorbance, molecular size distribution, and fluorescence character. In membrane filtration tests, unified membrane fouling index (UMFI) and hydraulic resistance were used to quantify irreversible fouling potential of different water samples. Results show that under similar DOC level in feed water, colloids present much more irreversible fouling than other fractions. The fouling effect of the isolates is related to their size, chemical properties, and solution chemistry. Further investigations have identified that the interaction between colloids and other fractions also influences the performance of colloids in fouling phenomena. © 2014 Elsevier Ltd.

  8. Assembling consumption

    DEFF Research Database (Denmark)

    Assembling Consumption marks a definitive step in the institutionalisation of qualitative business research. By gathering leading scholars and educators who study markets, marketing and consumption through the lenses of philosophy, sociology and anthropology, this book clarifies and applies...... the investigative tools offered by assemblage theory, actor-network theory and non-representational theory. Clear theoretical explanation and methodological innovation, alongside empirical applications of these emerging frameworks will offer readers new and refreshing perspectives on consumer culture and market...... societies. This is an essential reading for both seasoned scholars and advanced students of markets, economies and social forms of consumption....

  9. Práticas de leitura em meio eletrônico/Reading practices in electronic media

    Directory of Open Access Journals (Sweden)

    Leilah Santiago Bufrem

    2009-12-01

    Full Text Available Discute a relação livro universitário, leitor e tecnologias, para caracterizar como os estudantes percebem as possibilidades de leitura promovidas pelo livro eletrônico. Busca entender as práticas desse leitor em relação ao novo formato do livro, analisando inicialmente um referencial teórico, para fundamentar a investigação empírica. Aplica um questionário a um conjunto de estudantes, procurando identificar características como freqüência de leitura e tempo a ela dedicado, práticas de leitura, de acordo com o suporte, os dispositivos, o gênero e o acesso. Observa que a leitura é realizada pela maioria dos estudantes na própria tela do computador, ou após a impressão do conteúdo em papel, o que corrobora afirmações obtidas na literatura. Quanto aos aparelhos de leitura, a maioria não os conhece e aqueles que conhecem não os adquiriram. Sobre o tempo destinado à leitura, ratifica o que já se tem sido constatado em pesquisas sobre essa prática, ou seja, o estudante lê pouco, em média 6,5 horas por semana entre os mestrandos e 3,67 entre os graduandos, índice relacionado a todos os tipos de leitura e suporte, em papel ou eletrônico. Conclui que embora ainda não utilizado com freqüência e até pouco apreciado, o livro eletrônico é objeto de expectativas de aperfeiçoamento evidentes, enquanto editoras inovam e criam serviços e produtos compatíveis à evolução desse campo, procurando atender a demanda pelos formatos alternativos de publicação. This work discusses the relation among university book, reader and technologies, with the aim of identify how students perceive the reading possibilities fostered by the electronic book. It seeks to understand this reader’s practices towards the new book format, initially analyzing a theoretical reference, to ground the empirical survey. It submits a questionnaire to a group of students, trying to identify characteristics such as reading frequency and time spent in

  10. Executive function (EF) in dyslexia: examining an EF profile associated with dyslexia and comorbid dyslexia-ADHD and exploring the near and far transfer effects of EF training in dyslexia alone.

    OpenAIRE

    Doyle, Caoilainn

    2017-01-01

    Although there are several competing theories to explain dyslexia, no clear causal pathway has been established. Current theories also fail to address associated socio-emotional difficulties and high co-occurrence of dyslexia with ADHD. Executive function (EF), an umbrella term for a triad of high-level cognitive processes associated with pre-frontal brain regions – response inhibition (RI), working memory updating and switching, is a candidate factor for explaining the overlap between dyslex...

  11. Desenvolvimento de um penetrômetro manual eletrônico = Development of an electronic manual penetrometer

    Directory of Open Access Journals (Sweden)

    Rafael Cesar Tieppo

    2011-01-01

    Full Text Available O excesso de tráfego de máquinas pode causar compactação prejudicial ao desenvolvimento da cultura. Uma das maneiras de se obter um indicativo de compactação é a resistência do solo à penetração (RSP. Assim, desenvolveu-se um penetrômetro eletrônico, seguindo a norma S313.2 ASAE (1998, tendo a haste 0,0095 m de diâmetro e o cone de 30° com 0,01283 m de diâmetro na base. Para o desenvolvimento do penetrômetro, utilizou-se um sistema para aquisição de dados, sensor de distância, sensor de força e barras circulares de aço inox para confecção da haste e do cone. O aparelho armazena os dados coletados a campo em uma frequência de 4 Hz, sendo estes posteriormente transferidos para um microcomputador, via interface RS232. Por meio de uma planilha eletrônica, os dados podem ser processados de acordo com a necessidade do usuário. Os sensores apresentaram confiabilidade em sua utilização, os arquivos de dados gerados pelo aparelhoproporcionaram conforto e confiança no processamento de dados e os dados puderam ser reorganizados conforme a necessidade do usuário para, assim, evitar o erro de leitura pelo processo visual.Excess machine traffic can cause compaction that is harmful for crop growth. One way to obtain evidence of soil compaction is to check the mechanical resistance of soil. Thus, an electronic penetrometer was developed according to ASAE (1998 Standard S313.2, featuring a 9.53mm wide diving shaft and a 30° circular stainless steel cone with a 12.83 mm base diameter. The penetrometer was outfitted with a data acquisition system, distance and force sensors, and stainless steel circular bars to construct the diving shaft and cone. The device stores field data collected at a frequency of 4 Hz, which are later transferred onto a microcomputer via a RS232 interface. The collected data can be processed using an electronic spreadsheet according to user needs. The sensors proved reliable during use, and the data files

  12. Dependência de jogos eletrônicos: a possibilidade de um novo diagnóstico psiquiátrico

    Directory of Open Access Journals (Sweden)

    Igor Lins Lemos

    2012-01-01

    Full Text Available CONTEXTO: A prática de jogos eletrônicos vem se desconectando do caráter meramente lúdico, podendo seu uso excessivo ser considerado um novo transtorno psiquiátrico. OBJETIVO: Realizar uma revisão da literatura científica a respeito dessa possível dependência, abordando suas principais características, a prevalência em âmbito mundial e a possibilidade de comorbidades, e avaliar a existência de procedimentos de tratamento baseados na terapia cognitivo-comportamental. MÉTODO: O estudo foi realizado nos bancos de dados da PubMed, BVS, Lilacs e SciELO, no período de 2001 a junho de 2011. RESULTADOS: Não há consenso se a dependência de jogos eletrônicos pertence ao grupo do transtorno do controle dos impulsos ou de dependência química. É visto que a prevalência dessa dependência ao redor do mundo tem média de 3%, com predominância do sexo masculino. Foram encontradas comorbidades relacionadas à dependência de jogos eletrônicos. A terapia cognitivo-comportamental já é utilizada como possível tratamento, com resultados eficazes em curto prazo. CONCLUSÃO: Apesar de a dependência de jogos eletrônicos ainda não possuir um diagnóstico específico, esse novo fenômeno deve ser discutido e aprofundado na literatura científica, buscando-se, paralelamente, um modelo eficiente de tratamento psicoterapêutico e farmacológico.

  13. Ecological Footprint of Biological Resource Consumption in a Typical Area of the Green for Grain Project in Northwestern China

    Directory of Open Access Journals (Sweden)

    Jie Hu

    2015-01-01

    Full Text Available Following the implementation of the Green for Grain Project in 2000 in Guyuan, China, the decrease in cultivated land and subsequent increase in forest and grassland pose substantial challenges for the supply of biological products. Whether the current biologically productive land-use patterns in Guyuan satisfy the biological product requirements for local people is an urgent problem. In this study, the ecological footprints of biological resource consumption in Guyuan were calculated and analyzed based on the ‘City Hectare’ Ecological Footprint (EF Method. The EFs of different types of biological resource products consumed from different types of biologically productive land were then analyzed. In addition, the EFs of various biological resource products before and after the implementation of the Green for Grain Project (1998 and 2012 were assessed. The actual EF and bio-capacity (BC were compared, and differences in the EF and BC for different types of biologically productive lands before and after the project were analyzed. The results showed that the EF of Guyuan’s biological resource products was 0.65866 ha/cap, with an EF outflow and EF inflow of 0.2280 ha/cap and 0.0951 ha/cap, respectively. The per capita EF of Guyuan significantly decreased after the project, as did the ecological deficit. Whereas the cultivated land showed a deficit, grasslands were characterized by ecological surplus. The total EF of living resource consumption in Guyuan was 810,941 ha, and the total BC was 768,065 ha. In additional to current biological production areas, approximately 42,876 ha will be needed to satisfy the demands of Guyuan’s people. Cultivated land is the main type of biologically productive land that is needed.

  14. Collaborative Consumption

    DEFF Research Database (Denmark)

    Gjerdrum Pedersen, Esben Rahbek; Netter, Sarah

    2015-01-01

    Purpose – The purpose of this paper is to explore barriers and opportunities for business models based on the ideas of collaborative consumption within the fashion industry. Design/methodology/approach – The analysis is based on a multiple-case study of Scandinavian fashion libraries – a new...... to the new phenomenon of fashion libraries and does not cover other types of collaborative consumption within the fashion industry (Swap-parties, etc.). Originality/value – The paper is one of the first attempts to examine new business models of collaborative consumption in general and the fashion library...... concept in particular. The study contributes to the discussions of whether and how fashion sharing and collaboration holds promise as a viable business model and as a means to promote sustainability....

  15. Collaborative Consumption

    DEFF Research Database (Denmark)

    Gjerdrum Pedersen, Esben Rahbek; Netter, Sarah

    Purpose: The purpose of this paper is to explore barriers and opportunities for business models based on the ideas of collaborative consumption within the fashion industry. Design/methodology/approach: The analysis is based on a multiple-­‐‑case study of Scandinavian fashion libraries – a new...... to the new phenomenon of fashion libraries and does not cover other types of collaborative consumption within the fashion industry (Swap-­‐‑parties, etc.). Originality/value: The paper is one of the first attempts to examine new business models of collaborative consumption in general and the fashion library...... concept in particular. The study contributes to the discussions of whether and how fashion sharing and collaboration holds promise as a viable business model and as a means to promote sustainability....

  16. Positioning consumption

    DEFF Research Database (Denmark)

    Halkier, Bente; Keller, Margit

    2014-01-01

    positionings emerges based on empirical examples of research in parent–children consumption. Positionings are flexible discursive fixations of the relationship between the performances of the practitioner, other practitioners, media discourse and consumption activities. The basic positioning types...... are the practice maintenance and the practice change position, with different sorts of adapting in between. Media discourse can become a resource for a resistant position against social control or for an appropriating position in favour of space for action. Regardless of the current relation to a particular media......This article analyses the ways in which media discourses become a part of contested consumption activities. We apply a positioning perspective with practice theory to focus on how practitioners relate to media discourse as a symbolic resource in their everyday practices. A typology of performance...

  17. Lean consumption.

    Science.gov (United States)

    Womack, James P; Jones, Daniel T

    2005-03-01

    During the past 20 years, the real price of most consumer goods has fallen worldwide, the variety of goods and the range of sales channels offering them have continued to grow, and product quality has steadily improved. So why is consumption often so frustrating? It doesn't have to be--and shouldn't be--the authors say. They argue that it's time to apply lean thinking to the processes of consumption--to give consumers the full value they want from goods and services with the greatest efficiency and the least pain. Companies may think they save time and money by off-loading work to the consumer but, in fact, the opposite is true. By streamlining their systems for providing goods and services, and by making it easier for customers to buy and use those products and services, a growing number of companies are actually lowering costs while saving everyone time. In the process, these businesses are learning more about their customers, strengthening consumer loyalty, and attracting new customers who are defecting from less user-friendly competitors. The challenge lies with the retailers, service providers, manufacturers, and suppliers that are not used to looking at total cost from the standpoint of the consumer and even less accustomed to working with customers to optimize the consumption process. Lean consumption requires a fundamental shift in the way companies think about the relationship between provision and consumption, and the role their customers play in these processes. It also requires consumers to change the nature of their relationships with the companies they patronize. Lean production has clearly triumphed over similar obstacles in recent years to become the dominant global manufacturing model. Lean consumption, its logical companion, can't be far behind.

  18. Analysis of the impact of studies published by Internext - Revista Eletrônica de Negócios Internacionais

    Directory of Open Access Journals (Sweden)

    Carlos Luis González-Valiente

    2015-08-01

    Full Text Available This is a citation analysis of the journal InternexT-Revista Eletrônica de Negócios Internacionais to determine the impact caused by studies published from 2006 to 2013. The software Publish or Perish (PoP was used, which retrieves articles and citations from the Google Scholar database. Among the indicators applied were: distribution of authors per article, citations per year, citation versus self-citation, citable journals versus non-citable documents, citation of the journal versus uncited documents, co-word analysis and h-index. 131 articles were obtained with a total of 153 citations received until June 2014. The articles tend to have multiple authors and an upward trend in citations was detected. The levels of self-citation were very low and most of the citing sources are Brazilian journals. There were more uncited than cited articles and those published during the first years (2006-2008 caused a larger impact.  The most cited articles are related to the topics of internationalization theory and strategy, transaction analysis and corporative governance. The h-index according to PoP is 6 and the g-index is 9.

  19. A globalização, a OMC e o comércio eletrônico

    Directory of Open Access Journals (Sweden)

    Alexandre Dias Pereira

    2002-12-01

    Full Text Available Os interesses do comércio mundialque presidem à actuação da OMC estão a tomarconta da Internet. Neste trabalho, procura-se denunciar o mito de comércio eletrônicolivre, através da análise da substituição das taxaspúblicas pelas rendas privadas no ADPIC/TRIPS, em especial no que respeita ao direitode distribuição e às exceções aos direitos depropriedade intelectual, por um lado, e dasutileza do neoprotecionismo, ora a propósitoda proteção do consumidor ora a propósito daproteção da saúde pública, por outro.Abstract: The interests of the world trade,that the WTO protects, are taking over theInternet. This paper is an essay to denouncethe myth of a free e-commerce by theanalysis of the replacement of public taxesby private royalties, in particular in whatconcerns the right of distribution and theexceptions to intellectual property rights, onone hand, and the subtleness of the neoprotectionismregarding consumer andpublic health protection.

  20. Publicação Eletrônica: a linguagem do e-book na hipermídia

    Directory of Open Access Journals (Sweden)

    Daniela Carvalho Monteiro Ferreira

    2013-12-01

    Full Text Available O e-Book é um ambiente virtual e os formatos ePub e Daisy são um conjunto de arquivos interconectados, com potencial de utilização de fontes externas de informação, o que os tornam uma hipermídia. O objetivo deste artigo é apresentar os primeiros resultados sobre a utilização do quadro metodológico de planejamento do conteúdo audiovisual na edição de e-Book. Verificou-se que, assim como na web, os formatos ePub e Daisy, e em especial o primeiro, possui o potencial de utilização de recursos audiovisuais que podem fazer do resultado extrapolar a linguagem tradicional do livro impresso, tornando-os multimídia. Surge então o questionamento sobre o papel do editor de e-Book enquanto publicação eletrônica baseado no hipertexto, que assemelha-se com o de um roteirista para hipermídia.

  1. Uso de sintagmas nominais na classificação automática de documentos eletrônicos

    Directory of Open Access Journals (Sweden)

    Luiz Cláudio Maia

    Full Text Available Esta pesquisa verificou se ocorre aprimoramento na classificação de documentos eletrônicos com o uso de técnicas e algoritmos de mineração de texto (análise de texto utilizando-se, além das palavras, sintagmas nominais como indexadores. Utilizaram-se duas ferramentas nos experimentos propostos desta pesquisa o OGMA e a WEKA. O OGMA foi desenvolvido pelos autores para automatizar a extração dos sintagmas nominas e o cálculo do peso de cada termo na indexação dos documentos para cada um dos seis métodos propostos. A WEKA foi utilizada para analisar os resultados encontrados pelo OGMA utilizando aos algoritmos de agrupamento e classificação, SimpleKMeans e NaiveBayes, respectivamente, obtendo um valor percentual indicando quantos documentos foram classificados corretamente. Os métodos com melhores resultados foram o de termos sem stopwords e o de sintagmas nominais classificados e pontuados como descritores.

  2. eEF-2 Phosphorylation Down-Regulates P-Glycoprotein Over-Expression in Rat Brain Microvessel Endothelial Cells.

    Directory of Open Access Journals (Sweden)

    Xing Hua Tang

    Full Text Available We investigated whether glutamate, NMDA receptors, and eukaryote elongation factor-2 kinase (eEF-2K/eEF-2 regulate P-glycoprotein expression, and the effects of the eEF-2K inhibitor NH125 on the expression of P-glycoprotein in rat brain microvessel endothelial cells (RBMECs.Cortex was obtained from newborn Wistar rat brains. After surface vessels and meninges were removed, the pellet containing microvessels was resuspended and incubated at 37°C in culture medium. Cell viability was assessed by the MTT assay. RBMECs were identified by immunohistochemistry with anti-vWF. P-glycoprotein, phospho-eEF-2, and eEF-2 expression were determined by western blot analysis. Mdr1a gene expression was analyzed by RT-PCR.Mdr1a mRNA, P-glycoprotein and phospho-eEF-2 expression increased in L-glutamate stimulated RBMECs. P-glycoprotein and phospho-eEF-2 expression were down-regulated after NH125 treatment in L-glutamate stimulated RBMECs.eEF-2K/eEF-2 should have played an important role in the regulation of P-glycoprotein expression in RBMECs. eEF-2K inhibitor NH125 could serve as an efficacious anti-multidrug resistant agent.

  3. Electrostatics effects on Ca(2+) binding and conformational changes in EF-hand domains: Functional implications for EF-hand proteins.

    Science.gov (United States)

    Ababou, Abdessamad; Zaleska, Mariola

    2015-12-01

    Mutations of Gln41 and Lys75 with nonpolar residues in the N-terminal domain of calmodulin (N-Cam) revealed the importance of solvation energetics in conformational change of Ca(2+) sensor EF-hand domains. While in general these domains have polar residues at these corresponding positions yet the extent of their conformational response to Ca(2+) binding and their Ca(2+) binding affinity can be different from N-Cam. Consequently, here we address the charge state of the polar residues at these positions. The results show that the charge state of these polar residues can affect substantially the conformational change and the Ca(2+) binding affinity of our N-Cam variants. Since all the variants kept their conformational activity in the presence of Ca(2+) suggests that the differences observed among them mainly originate from the difference in their molecular dynamics. Hence we propose that the molecular dynamics of Ca(2+) sensor EF-hand domains is a key factor in the multifunctional aspect of EF-hand proteins. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. Evolution of EF-hand calcium-modulated proteins. IV. Exon shuffling did not determine the domain compositions of EF-hand proteins

    Science.gov (United States)

    Kretsinger, R. H.; Nakayama, S.

    1993-01-01

    In the previous three reports in this series we demonstrated that the EF-hand family of proteins evolved by a complex pattern of gene duplication, transposition, and splicing. The dendrograms based on exon sequences are nearly identical to those based on protein sequences for troponin C, the essential light chain myosin, the regulatory light chain, and calpain. This validates both the computational methods and the dendrograms for these subfamilies. The proposal of congruence for calmodulin, troponin C, essential light chain, and regulatory light chain was confirmed. There are, however, significant differences in the calmodulin dendrograms computed from DNA and from protein sequences. In this study we find that introns are distributed throughout the EF-hand domain and the interdomain regions. Further, dendrograms based on intron type and distribution bear little resemblance to those based on protein or on DNA sequences. We conclude that introns are inserted, and probably deleted, with relatively high frequency. Further, in the EF-hand family exons do not correspond to structural domains and exon shuffling played little if any role in the evolution of this widely distributed homolog family. Calmodulin has had a turbulent evolution. Its dendrograms based on protein sequence, exon sequence, 3'-tail sequence, intron sequences, and intron positions all show significant differences.

  5. Unsustainable Consumption

    DEFF Research Database (Denmark)

    Thøgersen, John

    2014-01-01

    Our dominant way of living is not sustainable and our activities as private individuals and households directly and indirectly account for a large and increasing share of total environmental impacts. These impacts are related to the structure as well as the level of consumption. In this article, ...

  6. Consumption bomb.

    Science.gov (United States)

    Harrison, P

    1999-01-01

    This article focuses on the issue of consumption in relation to the growing world population. Over the past 25 years, world population increased by 53%, while world consumption per person increased by only 39%. If consumption continues to grow at 1.4%, the world consumption per person will rise by 100% over the next 50 years with the population increasing by only half that amount. The burden of reducing the environmental impact brought about by this increase lies on technology. Technology needs to deliver major changes in improving resource productivity, and decreasing the amount of waste created. Productivity such as global food production has kept up with demand. Malnutrition persists due to poverty, and not because of the inability of the world to produce enough food. However, the prospects are much worse for resources that are not traded on markets or subject to sustainable management such as groundwater, state forests, ocean fish, and communal waste sinks like rivers, lakes, and the global atmosphere. These resources are not under the direct control of people affected by shortage. People who want to change the way these resources are used or managed have to pass through the legal or political system. Usually, political responses are slow and there has to be a very widespread environmental damage before action is taken.

  7. Eletrólise com radiofrequência no tratamento da triquíase Treatment of trichiasis with high-frequency radio wave electrosurgery

    Directory of Open Access Journals (Sweden)

    Rodrigo Beraldi Kormann

    2007-03-01

    Full Text Available OBJETIVOS: Triquíase é uma condição adquirida dos cílios, que perdem o direcionamento normal e tocam a superfície ocular. O tratamento da triquíase tem sido um problema terapêutico. Apesar das várias modalidades de tratamento: epilação mecânica, eletrólise com bipolar, crioterapia, fotocoagulação com laser de argônio e procedimentos cirúrgicos, a recidiva da triquíase é comum. Com o objetivo de encontrar técnica próxima ao ideal, realizamos estudo utilizando eletrólise com aparelho de radiofreqüência, avaliando-se a efetividade da técnica. MÉTODOS: Foram estudados, prospectivamente, 34 pacientes (54 segmentos palpebrais com triquíase, submetidos à eletrólise com aparelho de radiofreqüência. Reavaliaram-se os pacientes em oito semanas, quanto à cura, recidiva e possíveis complicações. RESULTADOS: Observou-se a cura da triquíase com apenas uma única sessão de eletrólise com radiofreqüência em 22 pacientes e com duas ou mais sessões em 12 pacientes. CONCLUSÃO: A eletrólise com aparelho de radiofreqüência é método simples, de baixo custo, que não demanda experiência do cirurgião, com ausência de complicações relevantes e com alto índice de sucesso.PURPOSE: Trichiasis is an acquired condition in which the eyelashes are directed posteriorly, touching the surface of the cornea. It poses a complex therapeutic problem. Despite several therapeutic options (epilation, electroepilation, cryotherapy, laser ablation and standard surgical techniques, relapse is frequent. METHODS: A prospective, nonmasked study was conducted to determine the efficacy of high-frequency radioelectrosurgery for treatment of trichiasis. Thirty-four patients (fifty-four eyelid segments were treated with this technique. After eight weeks, all patients were reviewed regarding the success rate, relapse and complications. RESULTS: Cure was achieved after only one session of treatment in twenty-two patients, and two or more sessions

  8. Interleukin-11 binds specific EF-hand proteins via their conserved structural motifs.

    Science.gov (United States)

    Kazakov, Alexei S; Sokolov, Andrei S; Vologzhannikova, Alisa A; Permyakova, Maria E; Khorn, Polina A; Ismailov, Ramis G; Denessiouk, Konstantin A; Denesyuk, Alexander I; Rastrygina, Victoria A; Baksheeva, Viktoriia E; Zernii, Evgeni Yu; Zinchenko, Dmitry V; Glazatov, Vladimir V; Uversky, Vladimir N; Mirzabekov, Tajib A; Permyakov, Eugene A; Permyakov, Sergei E

    2017-01-01

    Interleukin-11 (IL-11) is a hematopoietic cytokine engaged in numerous biological processes and validated as a target for treatment of various cancers. IL-11 contains intrinsically disordered regions that might recognize multiple targets. Recently we found that aside from IL-11RA and gp130 receptors, IL-11 interacts with calcium sensor protein S100P. Strict calcium dependence of this interaction suggests a possibility of IL-11 interaction with other calcium sensor proteins. Here we probed specificity of IL-11 to calcium-binding proteins of various types: calcium sensors of the EF-hand family (calmodulin, S100B and neuronal calcium sensors: recoverin, NCS-1, GCAP-1, GCAP-2), calcium buffers of the EF-hand family (S100G, oncomodulin), and a non-EF-hand calcium buffer (α-lactalbumin). A specific subset of the calcium sensor proteins (calmodulin, S100B, NCS-1, GCAP-1/2) exhibits metal-dependent binding of IL-11 with dissociation constants of 1-19 μM. These proteins share several amino acid residues belonging to conservative structural motifs of the EF-hand proteins, 'black' and 'gray' clusters. Replacements of the respective S100P residues by alanine drastically decrease its affinity to IL-11, suggesting their involvement into the association process. Secondary structure and accessibility of the hinge region of the EF-hand proteins studied are predicted to control specificity and selectivity of their binding to IL-11. The IL-11 interaction with the EF-hand proteins is expected to occur under numerous pathological conditions, accompanied by disintegration of plasma membrane and efflux of cellular components into the extracellular milieu.

  9. Effects of Exergame Play on EF in Children and Adolescents at a Summer Camp for Low Income Youth.

    Science.gov (United States)

    Flynn, Rachel M; Richert, Rebekah A; Staiano, Amanda E; Wartella, Ellen; Calvert, Sandra L

    2014-01-01

    Past research has suggested exergame play improves adolescents' executive function (EF) skills. EF change in 70 African American and Hispanic/Latino 10- to 16-year-olds participating in an inner-city summer camp was assessed following five 30-minute exergame play sessions. Children's EF scores improved from pre- to posttest, and factors related to this change were examined. The number of exergame sessions the participants attended predicted posttest scores. In addition, level of achievement during game play was related to EF scores. Finally, the children's level of enjoyment was not related to EF; however, frustration and boredom during game play were negatively related to EF. The findings are discussed in terms of their implications for the relationship between exergame play and cognitive benefits for adolescent players.

  10. The Effect of dcEFs on migration behavior of A549 cells and Integrin beta1 expression

    Directory of Open Access Journals (Sweden)

    Yunjie WANG

    2008-04-01

    Full Text Available Background and objective The effect of direct-current electric fields (dcEFs on cells attracted extensive attention. Moreover the metastasis and its potential are considered to be related to dcEFs. The aim is to study the effect of dcEFs on migration behavior of A549 cells, Integrin ?1 and its signal pathways. Methods According to exposure to 5 V/cm dcEFs or not and the time of exposure, the A549 cells were divided into 4 groups. Images were taken per 5 min within 2 h to recode the migration of the cells. The data of results were analyzed statistically. Results Most of A549cells exposed to the dcEFs aligned and elongated perpendicularly to the electric field lines and migrated to the cathode continually during 2 h. On the contrary, cells unexposed to dcEFs showed slightly random movements. Immunofluorescence showed that Integrin ?1 on plasma membrane polarized to the cathode of the dcEFs. Western blot showed that Integrin beta1 downstream signal pathways p-FAK and p-ERK were overexpressed in the dcEFs. Conclusion A549 cells have a galvanotatic feature of cathodal directed migration while exposed to the dcEFs. The polarization of Integrin beta1 and the promotion of its downstream signal pathways may play an important roles in the galvanotaxis of A549 cells.

  11. Influence of activated carbon preloading by EfOM fractions from treated wastewater on adsorption of pharmaceutically active compounds.

    Science.gov (United States)

    Hu, Jingyi; Shang, Ran; Heijman, Bas; Rietveld, Luuk

    2016-05-01

    In this study, the preloading effects of different fractions of wastewater effluent organic matter (EfOM) on the adsorption of trace-level pharmaceutically active compounds (PhACs) onto granular activated carbon (GAC) were investigated. A nanofiltration (NF) membrane was employed to separate the EfOM by size, and two GACs with distinct pore structures were chosen for comparison. The results showed that preloading with EfOM substantially decreased PhAC uptake of the GACs; however, comparable PhAC adsorption capacities were achieved on GACs preloaded by feed EfOM and the NF-permeating EfOM. This indicates that: (1) the NF-rejected, larger EfOM molecules with an expectation to block the PhAC adsorption pores exerted little impact on the adsorbability of PhACs; (2) the smaller EfOM molecules present in the NF permeate contributed mainly to the decrease in PhAC uptake, mostly due to site competition. Of the two examined GACs, the wide pore-size-distributed GAC was found to be more susceptible to EfOM preloading than the microporous GAC. Furthermore, among the fourteen investigated PhACs, the negatively charged hydrophilic PhACs were generally subjected to a greater EfOM preloading impact. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Transdisciplinary Consumption

    Directory of Open Access Journals (Sweden)

    Sue L.T. McGregor

    2013-06-01

    Full Text Available For the past 100 years, research about consumption has stemmed from two main disciplines: (a consumer studies/consumer sciences (including consumer policy and education (a spin off from home economics and (b consumer behaviour research (a spin off from marketing. This paper focuses on these two disciplines because the results of their respective research are used to shape consumer policy and consumer protection legislation and regulations, marketplace competition policy and regulations, consumer product and service information, media coverage of consumer issues, consumer education curricula and pedagogy, and insights into an evolving consumer culture. This paper asks consumer studies/sciences and consumer behaviour scholars to embrace the transdisciplinary methodology in addition to the traditional empirical, interpretive and critical methodologies. It provides an overview of the four axioms of transdisciplinary methodology with examples to illustrate how consumer-related research would change to address the complex reality of 21st century consumption.

  13. Collaborative Consumption

    OpenAIRE

    Rahbek Gjerdrum Pedersen, Esben; Netter, Sarah

    2013-01-01

    Purpose: The purpose of this paper is to explore barriers and opportunities for business models based on the ideas of collaborative consumption within the fashion industry. Design/methodology/approach: The analysis is based on a multiple-­‐‑case study of Scandinavian fashion libraries – a new, clothes-­‐‑sharing concept that has emerged as a fashion niche within the last decade. Findings: It is concluded that fashion libraries offers interesting perspectives, e.g. by allow...

  14. Construção de uma fonte de corrente e de uma sonda para medida de condutividade pelo método da sonda de quatro pontas The construction of an eletrical currente source and of a probe for conductivity measurement by a four point probe method

    Directory of Open Access Journals (Sweden)

    Olacir Alves Araújo

    2003-10-01

    Full Text Available This paper describes the construction of an eletrical current source and of a probe to be used in the measurement of eletrical conductivity through a four-point probe method. These pieces of equipments can be obtained at the low price of US$ 50.00 and are adequate for eletrical conductivity measurements in the semiconductor range, that is from 10-1 to 10-6 S cm-1.

  15. Membrane Fouling Potential of Secondary Effluent Organic Matter (EfOM) from Conventional Activated Sludge Process

    KAUST Repository

    Wei, Chunhai; Amy, Gary L.

    2012-01-01

    Secondary effluent organic matter (EfOM) from a conventional activated sludge process was filtered through constant-pressure dead-end filtration tests with a sequential ultrafiltration (UF, molecular weight cut-off (MWCO) of 10k Dalton) and nanofiltration (NF, MWCO of 200 Dalton) array to investigate its membrane fouling potential. Advanced analytical methods including liquid chromatography with online carbon detection (LC-OCD) and fluorescent excitation-emission matrix (F-EEM) were employed for EfOM characterization. EfOM consisted of humic substances and building blocks, low molecular weight (LMW) neutrals, biopolymers (mainly proteins) and hydrophobic organics according to the sequence of their organic carbon fractions. The UF rejected only biopolymers and the NF rejected most humics and building blocks and a significant part of LMW neutrals. Simultaneous occurrence of cake layer and standard blocking during the filtration process of both UF and NF was identified according to constant-pressure filtration equations, which was possibly caused by the heterogeneous nature of EfOM with a wide MW distribution (several ten to several million Dalton). Thus the corresponding two fouling indices (kc for cake layer and ks for standard blocking) from UF and NF could characterize the fouling potential of macromolecular biopolymers and low to intermediate MW organics (including humics, building blocks, LMW neutrals), respectively. Compared with macromolecular biopolymers, low to intermediate MW organics exhibited a much higher fouling potential due to their lower molecular weight and higher concentration.

  16. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu.

    Science.gov (United States)

    Castro-Roa, Daniel; Garcia-Pino, Abel; De Gieter, Steven; van Nuland, Nico A J; Loris, Remy; Zenkin, Nikolay

    2013-12-01

    Fic proteins are ubiquitous in all of the domains of life and have critical roles in multiple cellular processes through AMPylation of (transfer of AMP to) target proteins. Doc from the doc-phd toxin-antitoxin module is a member of the Fic family and inhibits bacterial translation by an unknown mechanism. Here we show that, in contrast to having AMPylating activity, Doc is a new type of kinase that inhibits bacterial translation by phosphorylating the conserved threonine (Thr382) of the translation elongation factor EF-Tu, rendering EF-Tu unable to bind aminoacylated tRNAs. We provide evidence that EF-Tu phosphorylation diverged from AMPylation by antiparallel binding of the NTP relative to the catalytic residues of the conserved Fic catalytic core of Doc. The results bring insights into the mechanism and role of phosphorylation of EF-Tu in bacterial physiology as well as represent an example of the catalytic plasticity of enzymes and a mechanism for the evolution of new enzymatic activities.

  17. Murine elongation factor 1 alpha (EF-1 alpha) is posttranslationally modified by novel amide-linked ethanolamine-phosphoglycerol moieties. Addition of ethanolamine-phosphoglycerol to specific glutamic acid residues on EF-1 alpha

    International Nuclear Information System (INIS)

    Whiteheart, S.W.; Shenbagamurthi, P.; Chen, L.; Cotter, R.J.; Hart, G.W.

    1989-01-01

    Elongation Factor 1 alpha (EF-1 alpha), an important eukaryotic translation factor, transports charged aminoacyl-tRNA from the cytosol to the ribosomes during poly-peptide synthesis. Metabolic radiolabeling with [ 3 H] ethanolamine shows that, in all cells examined, EF-1 alpha is the major radiolabeled protein. Radiolabeled EF-1 alpha has an apparent Mr = 53,000 and a basic isoelectric point. It is cytosolic and does not contain N-linked oligosaccharides. Trypsin digestion of murine EF-1 alpha generated two major [ 3 H]ethanolamine-labeled peptides. Three peptides were sequenced and were identical to two distinct regions of the human EF-1 alpha protein. Blank sequencing cycles coinciding with glutamic acid in the human cDNA-derived sequence were also found to release [ 3 H]ethanolamine, and compositional analysis of these peptides confirmed the presence of glutamic acid. Dansylation analysis demonstrates that the amine group of the ethanolamine is blocked. These results indicate that EF-1 alpha is posttranslationally modified by the covalent attachment of ethanolamine via an amide bond to at least two specific glutamic acid residues (Glu-301 and Glu-374). The hydroxyl group of the attached ethanolamine was shown by mass spectrometry and compositional analysis, to be further modified by the addition of a phosphoglycerol unit. This novel posttranslational modification may represent an important alteration of EF-1 alpha, comparable to the regulatory effects of posttranslational methylation of EF-1 alpha lysine residues

  18. Flexible Consumption

    DEFF Research Database (Denmark)

    Holm Jacobsen, Peter; Pallesen, Trine

    This report presents the first findings from our qualitative study of consumer behaviour vis-à-vis flexible consumption. The main of objective of this report is to present our first round of data from Bornholm, and to assist the design of products/services designed in WP6. In the report, we adopt...... the perspective of the consumer: what does living in a demand response setup look like to participants – and what kinds of behaviour and interest motivate – and emerge from – their participation in EcoGrid 2.0....

  19. Indústria cultural e infância: estudo sobre formação de valores em crianças no universo do jogo eletrônico Cultural industry and childhood: a study about the building of values in children in the world of electronic games

    Directory of Open Access Journals (Sweden)

    Silvia Rosa Silva Zanolla

    2007-12-01

    Full Text Available Esse trabalho parte do pressuposto de que o jogo eletrônico influencia a formação cultural das crianças na contemporaneidade. Essa afirmação fundamenta-se na teoria crítica da sociedade, sobretudo, em um de seus principais pensadores, T. W. Adorno, que, em conjunto com M. Horkheimer, cunhou o conceito de indústria cultural em 1947. Elemento desta indústria, o jogo eletrônico, como produto de consumo, constitui-se como um instrumento de lazer e entretenimento, cujo alcance é cada vez mais amplo nas brincadeiras infantis. Isso significa que o conteúdo desses jogos e a identificação com seus personagens são vetores importantes de referência para analisar a constituição da identidade infantil e a emergência de valores na sociedade atual.This work starts from the assumption that electronic games influence today's children's cultural formation. This affirmation is based on the critical theory of society, especially by one of its main thinkers, T. W. Adorno, who, along with M. Horkheimer, created the concept of culture industry in 1947. As elements of this culture, and as consumption products, electronic games are an instrument for leisure and entertainment, with an increasingly higher reach in children's play. This means that the content of these games and children's identification with their characters are important reference points to analyze the constitution of children's identity and the urgent need for values in society today.

  20. Consumption governance toward more sustainable consumption

    NARCIS (Netherlands)

    Wahlen, S.; Dubuisson-Quellier, Sophie

    2018-01-01

    This article deliberates on strategies of consumption governance toward more sustainable consumption. We discuss theoretical concepts stemming from various social science perspectives to (1) promote more sustainable consumption, (2) compare strategies stemming from individualist understanding of

  1. Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein.

    Science.gov (United States)

    Defeu Soufo, Hervé Joël; Reimold, Christian; Linne, Uwe; Knust, Tobias; Gescher, Johannes; Graumann, Peter L

    2010-02-16

    We show that translation initiation factor EF-Tu plays a second important role in cell shape maintenance in the bacterium Bacillus subtilis. EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal element setting up rod cell shape. The localization of MreB and of EF-Tu is interdependent, but in contrast to the dynamic MreB filaments, EF-Tu structures are more static and may serve as tracks for MreB filaments. In agreement with this idea, EF-Tu and MreB interact in vivo and in vitro. Lowering of the EF-Tu levels had a minor effect on translation but a strong effect on cell shape and on the localization of MreB, and blocking of the function of EF-Tu in translation did not interfere with the localization of MreB, showing that, directly or indirectly, EF-Tu affects the cytoskeletal MreB structure and thus serves two important functions in a bacterium.

  2. Structure of eEF3 and the mechanism of transfer RNA release from the E-site

    DEFF Research Database (Denmark)

    Andersen, Christian Brix Folsted; Becker, T.; Blau, M.

    2006-01-01

    Elongation factor eEF3 is an ATPase, which, in addition to the two canonical factors eEF1A and eEF2, serves an essential function in the translation cycle of fungi. eEF3 is required for the binding of the aatRNA-eEF1A-GTP ternary complex to the ribosomal A-site and has been suggested to facilitate...... the clearance of deacyl-tRNA from the E-site. Here, we present the crystal structure of eEF3 showing that it consists of an N-terminal HEAT repeat domain, followed by a four-helix bundle and two ABC-type ATPase domains with a chromo-domain inserted in ABC2. Moreover, we present the cryo-EM structure of the ATP......-bound form of eEF3 in complex with the post-translocational state 80S ribosome from yeast. eEF3 uses an entirely new factor binding site near the ribosomal E-site, with the chromodomain stabilizing the ribosomal L1 stalk in an open conformation, thus, allowing tRNA release....

  3. As motivações para o passa-palavra eletrónico negativo por parte de consumidores que reclamam: um estudo exploratório

    OpenAIRE

    Gomes, Mariana Gouveia

    2017-01-01

    Dissertação de mestrado em Marketing e Estratégia Atualmente, o consumidor dispõe de diversas plataformas digitais onde pode partilhar todo o tipo de informação sem qualquer impedimento ou barreiras. Devido a essa facilidade, o passapalavra ganhou uma maior dimensão comparativamente ao que era habitual até ao surgimento da Web 2.0. O passa-palavra eletrónico tem um maior alcance do que o passa-palavra comum, não só porque atinge uma maior audiência, mas também porque fica dispo...

  4. Safe-record : segurança e privacidade para registros eletrônicos em saúde na nuvem

    OpenAIRE

    Souza, Stefano Mozart Pontes Canedo de

    2016-01-01

    O mercado de computação em nuvem tornou disponível a equipes hospitalares e acadêmicas um volume virtualmente ilimitado de recursos computacionais, os quais têm sido utilizados em atividades de pesquisa e desenvolvimento. Apesar das evidentes vantagens da computação em nuvem, o armazenamento e o processamento de Registros Eletrônicos em Saúde (RES) nesses ambientes levantam preocupações genuínas com a privacidade e a segurança dos pacientes que, voluntária ou involuntariamente, têm seus dados...

  5. Análise de fatores que influenciam o uso de serviços de governo eletrônico no Brasil

    OpenAIRE

    Marcelo Henrique de Araujo

    2013-01-01

    A presente investigação científica tem como objetivo compreender de que maneira o uso de serviços de governo eletrônico (e-gov) é influenciado pelos diferentes \\"locais de acesso à internet\\" e pelas competências de uso do internauta. Para tanto, foram utilizados os microdados da pesquisa TIC Domicílios (desenvolvida pelo Centro de Estudos sobre as Tecnologias da Informação e Comunicação) referente aos anos de 2007, 2009 e 2011, se caracterizado um estudo longitudinal. Os procedimentos refere...

  6. Limites organizacionais e relações na cadeia de suprimentos : estudo de caso de uma empresa da indústria eletrônica do consumo

    OpenAIRE

    Bittar, Alexandre de Vicente

    2010-01-01

    Trata o conceito de Cadeia de Suprimentos, especificamente através da análise de dois modelos teóricos, buscando verificar a aplicabilidade destes modelos em uma empresa da Indústria Eletrônica de Consumo. Aborda as decisões Make or Buy dentro deste conceito, explorando as variáveis que condicionam os Limites das Organizações. Infere qual o comportamento da empresa em relação às decisões de Integração Vertical ou Subcontratação de atividades no mercado, mapeando os fatores q...

  7. Acelerômetro eletrônico e a placa Arduino para ensino de física em tempo real

    OpenAIRE

    Fábio Saraiva da Rocha; Guilherme Frederico Maranghello; Márcia Maria Lucchese

    2013-01-01

    http://dx.doi.org/10.5007/2175-7941.2014v31n1p98 Apresenta-se o projeto de um acelerômetro eletrônico triaxial voltado ao ensino de física experimental. Este dispositivo, quando acoplado adequadamente a um corpo, permite a leitura da aceleração imprimida ao conjunto. O acelerômetro funciona conectado eletronicamente à placa Arduíno sendo capaz de fornecer dados numéricos de aceleração que atualizam no decorrer de uma experiência. Tais dados podem ser disponibilizados através de tabelas e ...

  8. Monitoramento eletrônico de presos no RS: uma leitura bakhtiniana do discurso do gênero reportagem na esfera jornalística

    OpenAIRE

    Rosa, Renata Faria Amaro da Silva da

    2015-01-01

    Este trabalho propõe uma análise de construção dos sentidos do discurso em reportagens sobre o monitoramento eletrônico de presos no Rio Grande do Sul (RS), a partir da análise de discursos impressos no Jornal Zero Hora (ZH) entre os meses de janeiro e maio do ano de 2013, na perspectiva da Teoria da Enunciação de Bakhtin e seu Círculo. A pesquisa, fundamentada na teoria dialógica bakhtiniana, de forma mais específica, busca identificar vozes discursivas que compõem o discurso, reconhecer ele...

  9. Planejamento de melhorias em lojas virtuais para comércio eletrônico na Internet sob a ótica do cliente

    OpenAIRE

    Marcos Luis Basso

    2006-01-01

    Este trabalho tem por objetivo o planejamento de melhorias em lojas virtuais de comércio eletrônico na Internet usando como ferramentas a pesquisa de mercado e o Desdobramento da Função Qualidade (QFD), baseado no modelo conceitual de Ribeiro et al. (2001) adaptado para serviços. A pesquisa de mercado visa identificar os itens da qualidade demandada pelos clientes, enquanto o emprego do QFD visa desdobrar os itens priorizados em características de qualidade para o planejamento de melhorias da...

  10. DJ Jesus Luz?! Controvérsias, capital subcultural e performance de gosto em uma cena de música eletrônica

    OpenAIRE

    Pereira de Sá, Simone; Universidade Federal Fluminense; Polivanov, Beatriz; Universidade Federal Fluminense

    2015-01-01

    O artigo tem como questão central discutir questões de gosto, valor, e disputas simbólicas (Bourdieu; 2008, Thornton;1996; Frith; 1996) de atores de uma cena de música eletrônica brasileira (Straw; 1991). Para tal, focamos nossa análise no caso do modelo Jesus Luz, que namorou Madonna e posteriormente se tornou DJ, suscitando uma série de polêmicas relacionadas à autenticidade de suas performances. O caso de Jesus Luz será tratado como uma controvérsia (Latour; 1994; 2005) que nos permite apr...

  11. Boletim eletrônico: um estudo de caso na biblioteca do instituto de física da universidade federal do rio de janeiro (IF/UFRJ

    Directory of Open Access Journals (Sweden)

    Robson da Silva Teixeira

    2013-08-01

    Full Text Available Este artigo trata da elaboração de um Boletim Eletrônico como ferramenta de enfrentamento aos desafios impostos pelas Novas Tecnologias da Informação e Comunicação na disseminação da informação nas Universidades Públicas Brasileiras, tendo como parâmetro um estudo de caso no setor de referência da Biblioteca Plínio Sussekind Rocha do Instituto de Física da Universidade Federal do Rio de Janeiro (IF/UFRJ.

  12. Dimensões de Consciência dos Consumidores no Processo de Reciclagem do Lixo Eletrônico (E-WASTE)

    OpenAIRE

    Pereira, Fernando Antonio de Melo; Ferraz, Sofia Batista; Massaini, Silvye Ane

    2014-01-01

    O crescente uso de artigos tecnológicos trouxe um novo tipo de lixo para o meio ambiente, os lixos eletroeletrônicos (e-waste) e o questionamento do que fazer com ele. Este estudo objetivou analisar as dimensões que compõem o comportamento de consumo de produtos eletrônicos e a relação com o nível de consciência no processo de reciclagem por alunos graduandos em Administração enquanto consumidores e futuros gestores. Trata-se de uma pesquisa quantitativa com 166 respondentes, com uso de técni...

  13. Up-regulation of eEF1A2 promotes proliferation and inhibits apoptosis in prostate cancer

    International Nuclear Information System (INIS)

    Sun, Yue; Du, Chengli; Wang, Bo; Zhang, Yanling; Liu, Xiaoyan; Ren, Guoping

    2014-01-01

    Highlights: • The expression of eEF1A2 is up-regulated in prostate cancer tissues. • Suppression of eEF1A2 inhibits the proliferation and promotes apoptosis. • Inhibition of eEF1A2 enhances the expression of apoptotic relevant proteins. • The expressions of eEF1A2 and cleavage-caspase3 are inversely correlated. - Abstract: Background: eEF1A2 is a protein translation factor involved in protein synthesis, which possesses important function roles in cancer development. This study aims at investigating the expression pattern of eEF1A2 in prostate cancer and its potential role in prostate cancer development. Methods: We examined the expression level of eEF1A2 in 30 pairs of prostate cancer tissues by using RT-PCR and immunohistochemical staining (IHC). Then we applied siRNA specifically targeting eEF1A2 to down-regulate its expression in DU-145 and PC-3 cells. Flow cytometer was used to explore apoptosis and Western-blot was used to detect the pathway proteins of apoptosis. Results: Our results showed that the expression level of eEF1A2 in prostate cancer tissues was significantly higher compared to their corresponding normal tissues. Reduction of eEF1A2 expression in DU-145 and PC-3 cells led to a dramatic inhibition of proliferation accompanied with enhanced apoptosis rate. Western blot revealed that apoptosis pathway proteins (caspase3, BAD, BAX, PUMA) were significantly up-regulated after suppression of eEF1A2. More importantly, the levels of eEF1A2 and caspase3 were inversely correlated in prostate cancer tissues. Conclusion: Our data suggests that eEF1A2 plays an important role in prostate cancer development, especially in inhibiting apoptosis. So eEF1A2 might serve as a potential therapeutic target in prostate cancer

  14. Function and structure in phage Qbeta RNA replicase. Association of EF-Tu-Ts with the other enzyme subunits

    DEFF Research Database (Denmark)

    Blumenthal, T; Young, R A; Brown, S

    1976-01-01

    alters its quaternary structure: the EF-Tu-Ts cannot be covalently attached to the other enzyme subunits with bifunctional cross-linking reagents in the presence of RNA. This conformational change is not influenced by ionic strength. The addition of Qbeta RNA to the enzyme, does not result in the release...... for one another increases with increasing ionic strength. The enzyme is capable of initiation of RNA synthesis with synthetic templates only when in the low ionic strength conformation. Elongation of initiated polynucleotide chains is not affectedby ionic strength. Addition of Qbeta RNA to the enzyme also...... of EF-Tu-Ts from the other enzyme subunits: whereas free EF-Tu-Ts binds GDP independently of salt concentration, this binding by Qbeta replicase is sensitive to high ionic strength and remains so in the presence of Qbeta RNA. Furthermore, RNA does not allow the release of EF-Ts from EF-Tu by GTP...

  15. Sustainable consumption

    DEFF Research Database (Denmark)

    Prothero, Andrea; Dobscha, Susan; Freund, Jim

    2011-01-01

    This essay explores sustainable consumption and considers possible roles for marketing and consumer researchers and public policy makers in addressing the many sustainability challenges that pervade our planet. Future research approaches to this interdisciplinary topic need to be comprehensive...... and systematic and will benefit from a variety of different perspectives. There are a number of opportunities for future research, and three areas are explored in detail. First, the essay considers the inconsistency between the attitudes and behaviors of consumers with respect to sustainability; next, the agenda...... is broadened to explore the role of individual citizens in society; and finally, a macro institutional approach to fostering sustainability is explored. Each of these areas is examined in detail and possible research avenues and public policy initiatives are considered within each of these separate...

  16. Expression of executive control in situational context: Effects of facilitating versus restraining cues on snack food consumption.

    Science.gov (United States)

    Hall, Peter; Tran, Betty; Lowe, Cassandra; Vincent, Corita; Mourtzakis, Marina; Liu-Ambrose, Teresa; Prapavessis, Harry; Gidron, Yori

    2015-05-01

    To examine the effects of executive function (EF) on objectively measured high-calorie snack food consumption in 2 age groups and to explore the moderating influence of environmental cues. In Study 1, 43 older adults (M(age) = 74.81) and in Study 2, 79 younger adults (M(age) = 18.71) completed measures of EF and subsequently participated in a bogus taste-test paradigm wherein they were required to rate 3 highly appetitive (but high-calorie) snack foods on taste and texture. Grams of snack food consumed was measured covertly in the presence randomly assigned contextual cues (explicit semantic cues in Study 1; implicit visual cues in Study 2) that were facilitating or restraining in nature. Findings indicated that in both age groups, stronger EF predicted lower consumption of snack foods across conditions, and the effects of EF were most pronounced in the presence of facilitating cues. Older and younger adults with weaker EF tend to consume more high-calorie snack food compared with their stronger EF counterparts. These tendencies appear to be especially amplified in the presence of facilitating cues. (c) 2015 APA, all rights reserved).

  17. Segurança na internet: a percepção do usuário como fator de restrição ao crescimento do comércio eletrônico no Brasil

    OpenAIRE

    Cernev, Adrian Kemmer

    2010-01-01

    O comércio eletrônico já é uma realidade brasileira. Contudo, esta modalidade de negócio eletrônico ainda não atingiu o seu pleno potencial, especialmente nas negociações orientadas para o consumidor (B2C). Vários fatores são apontados como restrições ao seu crescimento, mas nenhum deles é tão destacado e controvertido quanto a segurança na Internet, especialmente nas transações eletrônicas. Este trabalho analisa a questão da segurança do ponto de vista dos usuários de Internet...

  18. Dietary Fat, Sugar Consumption, and Cardiorespiratory Fitness in Patients With Heart Failure With Preserved Ejection Fraction

    Directory of Open Access Journals (Sweden)

    Salvatore Carbone, MS

    2017-10-01

    Full Text Available Heart failure with preserved ejection fraction (HFpEF is associated with obesity and, indirectly, with unhealthy diet. The role of dietary components in HFpEF is, however, largely unknown. In this study, the authors showed that in obese HFpEF patients, consumption of unsaturated fatty acids (UFA, was associated with better cardiorespiratory fitness, and UFA consumption correlated with better diastolic function and with greater fat-free mass. Similarly, mice fed with a high-fat diet rich in UFA and low in sugars had preserved myocardial function and reduced weight gain. Randomized clinical trials increasing dietary UFA consumption and reducing sugar consumption are warranted to confirm and expand our findings.

  19. Liposome-encapsulated EF24-HPβCD inclusion complex: a preformulation study and biodistribution in a rat model

    International Nuclear Information System (INIS)

    Agashe, H.; Lagisetty, P.; Sahoo, K.; Bourne, D.; Grady, B.; Awasthi, V.

    2011-01-01

    3,5-Bis(2-fluorobenzylidene)-4-piperidone (EF24) is an anti-proliferative diphenyldifluoroketone analog of curcumin with more potent activity. The authors describe a liposome preparation of EF24 using a “drug-in-CD-in liposome” approach. An aqueous solution of EF24 and hydroxypropyl-β-cyclodextrin (HPβCD) inclusion complex (IC) was used to prepare EF24 liposomes. The liposome size was reduced by a combination of multiple freeze–thaw cycles. Co-encapsulation of glutathione inside the liposomes conferred them with the capability of labeling with imageable radionuclide Tc-99m. Phase solubility analysis of EF24-HPβCD mixture provided k 1:1 value of 9.9 M −1 . The enhanced aqueous solubility of EF24 (from 1.64 to 13.8 mg/mL) due to the presence of HPβCD helped in the liposome preparation. About 19% of the EF24 IC was encapsulated inside the liposomes (320.5 ± 2.6 nm) by dehydration–rehydration technique. With extrusion technique, the size of 177 ± 6.5 nm was obtained without any effect on encapsulation efficiency. The EF24-liposomes were evaluated for anti-proliferative activity in lung adenocarcinoma H441 and prostate cancer PC-3 cells. The EF24-liposomes demonstrated anti-proliferative activity superior to that of plain EF24 at 10 μM dose. When injected in rats, the Tc-99m-labeled EF24-liposomes cleared from blood with an α-t 1/2 of 21.4 min and β-t 1/2 of 397 min. Tissue radioactivity counting upon necropsy showed that the majority of clearance was due to the uptake in liver and spleen. The results suggest that using “drug-in-CD-in liposome” approach is a feasible strategy to formulate an effective parenteral preparation of EF24. In vitro studies show that the liposomal EF24 remains anti-proliferative, while presenting an opportunity to image its biodistribution.

  20. Liposome-encapsulated EF24-HPβCD inclusion complex: a preformulation study and biodistribution in a rat model

    Science.gov (United States)

    Agashe, H.; Lagisetty, P.; Sahoo, K.; Bourne, D.; Grady, B.; Awasthi, V.

    2011-06-01

    3,5-Bis(2-fluorobenzylidene)-4-piperidone (EF24) is an anti-proliferative diphenyldifluoroketone analog of curcumin with more potent activity. The authors describe a liposome preparation of EF24 using a "drug-in-CD-in liposome" approach. An aqueous solution of EF24 and hydroxypropyl-β-cyclodextrin (HPβCD) inclusion complex (IC) was used to prepare EF24 liposomes. The liposome size was reduced by a combination of multiple freeze-thaw cycles. Co-encapsulation of glutathione inside the liposomes conferred them with the capability of labeling with imageable radionuclide Tc-99m. Phase solubility analysis of EF24-HPβCD mixture provided k 1:1 value of 9.9 M-1. The enhanced aqueous solubility of EF24 (from 1.64 to 13.8 mg/mL) due to the presence of HPβCD helped in the liposome preparation. About 19% of the EF24 IC was encapsulated inside the liposomes (320.5 ± 2.6 nm) by dehydration-rehydration technique. With extrusion technique, the size of 177 ± 6.5 nm was obtained without any effect on encapsulation efficiency. The EF24-liposomes were evaluated for anti-proliferative activity in lung adenocarcinoma H441 and prostate cancer PC-3 cells. The EF24-liposomes demonstrated anti-proliferative activity superior to that of plain EF24 at 10 μM dose. When injected in rats, the Tc-99m-labeled EF24-liposomes cleared from blood with an α- t 1/2 of 21.4 min and β- t 1/2 of 397 min. Tissue radioactivity counting upon necropsy showed that the majority of clearance was due to the uptake in liver and spleen. The results suggest that using "drug-in-CD-in liposome" approach is a feasible strategy to formulate an effective parenteral preparation of EF24. In vitro studies show that the liposomal EF24 remains anti-proliferative, while presenting an opportunity to image its biodistribution.

  1. Liposome-encapsulated EF24-HP{beta}CD inclusion complex: a preformulation study and biodistribution in a rat model

    Energy Technology Data Exchange (ETDEWEB)

    Agashe, H; Lagisetty, P; Sahoo, K; Bourne, D [University of Oklahoma Health Sciences Center, Department of Pharmaceutical Sciences (United States); Grady, B [School of Chemical, Biological and Materials Engineering (United States); Awasthi, V [University of Oklahoma Health Sciences Center, Department of Pharmaceutical Sciences (United States)

    2011-06-15

    3,5-Bis(2-fluorobenzylidene)-4-piperidone (EF24) is an anti-proliferative diphenyldifluoroketone analog of curcumin with more potent activity. The authors describe a liposome preparation of EF24 using a 'drug-in-CD-in liposome' approach. An aqueous solution of EF24 and hydroxypropyl-{beta}-cyclodextrin (HP{beta}CD) inclusion complex (IC) was used to prepare EF24 liposomes. The liposome size was reduced by a combination of multiple freeze-thaw cycles. Co-encapsulation of glutathione inside the liposomes conferred them with the capability of labeling with imageable radionuclide Tc-99m. Phase solubility analysis of EF24-HP{beta}CD mixture provided k{sub 1:1} value of 9.9 M{sup -1}. The enhanced aqueous solubility of EF24 (from 1.64 to 13.8 mg/mL) due to the presence of HP{beta}CD helped in the liposome preparation. About 19% of the EF24 IC was encapsulated inside the liposomes (320.5 {+-} 2.6 nm) by dehydration-rehydration technique. With extrusion technique, the size of 177 {+-} 6.5 nm was obtained without any effect on encapsulation efficiency. The EF24-liposomes were evaluated for anti-proliferative activity in lung adenocarcinoma H441 and prostate cancer PC-3 cells. The EF24-liposomes demonstrated anti-proliferative activity superior to that of plain EF24 at 10 {mu}M dose. When injected in rats, the Tc-99m-labeled EF24-liposomes cleared from blood with an {alpha}-t{sub 1/2} of 21.4 min and {beta}-t{sub 1/2} of 397 min. Tissue radioactivity counting upon necropsy showed that the majority of clearance was due to the uptake in liver and spleen. The results suggest that using 'drug-in-CD-in liposome' approach is a feasible strategy to formulate an effective parenteral preparation of EF24. In vitro studies show that the liposomal EF24 remains anti-proliferative, while presenting an opportunity to image its biodistribution.

  2. Liposome-encapsulated EF24-HP{beta}CD inclusion complex: a preformulation study and biodistribution in a rat model

    Energy Technology Data Exchange (ETDEWEB)

    Agashe, H.; Lagisetty, P.; Sahoo, K.; Bourne, D. [University of Oklahoma Health Sciences Center, Department of Pharmaceutical Sciences (United States); Grady, B. [School of Chemical, Biological and Materials Engineering (United States); Awasthi, V., E-mail: vawasthi@ouhsc.edu [University of Oklahoma Health Sciences Center, Department of Pharmaceutical Sciences (United States)

    2011-06-15

    3,5-Bis(2-fluorobenzylidene)-4-piperidone (EF24) is an anti-proliferative diphenyldifluoroketone analog of curcumin with more potent activity. The authors describe a liposome preparation of EF24 using a 'drug-in-CD-in liposome' approach. An aqueous solution of EF24 and hydroxypropyl-{beta}-cyclodextrin (HP{beta}CD) inclusion complex (IC) was used to prepare EF24 liposomes. The liposome size was reduced by a combination of multiple freeze-thaw cycles. Co-encapsulation of glutathione inside the liposomes conferred them with the capability of labeling with imageable radionuclide Tc-99m. Phase solubility analysis of EF24-HP{beta}CD mixture provided k{sub 1:1} value of 9.9 M{sup -1}. The enhanced aqueous solubility of EF24 (from 1.64 to 13.8 mg/mL) due to the presence of HP{beta}CD helped in the liposome preparation. About 19% of the EF24 IC was encapsulated inside the liposomes (320.5 {+-} 2.6 nm) by dehydration-rehydration technique. With extrusion technique, the size of 177 {+-} 6.5 nm was obtained without any effect on encapsulation efficiency. The EF24-liposomes were evaluated for anti-proliferative activity in lung adenocarcinoma H441 and prostate cancer PC-3 cells. The EF24-liposomes demonstrated anti-proliferative activity superior to that of plain EF24 at 10 {mu}M dose. When injected in rats, the Tc-99m-labeled EF24-liposomes cleared from blood with an {alpha}-t{sub 1/2} of 21.4 min and {beta}-t{sub 1/2} of 397 min. Tissue radioactivity counting upon necropsy showed that the majority of clearance was due to the uptake in liver and spleen. The results suggest that using 'drug-in-CD-in liposome' approach is a feasible strategy to formulate an effective parenteral preparation of EF24. In vitro studies show that the liposomal EF24 remains anti-proliferative, while presenting an opportunity to image its biodistribution.

  3. Joint resistance measurements of pancake and terminal joints for JT-60SA EF coils

    Energy Technology Data Exchange (ETDEWEB)

    Obana, Tetsuhiro, E-mail: obana.tetsuhiro@LHD.nifs.ac.jp [National Institute for Fusion Science, 322-6 Oroshi, Toki, Gifu 509-5292 (Japan); Takahata, Kazuya; Hamaguchi, Shinji; Mito, Toshiyuki; Imagawa, Shinsaku [National Institute for Fusion Science, 322-6 Oroshi, Toki, Gifu 509-5292 (Japan); Kizu, Kaname; Murakami, Haruyuki; Yoshida, Kiyoshi [Japan Atomic Energy Agency, 801-1 Mukoyama, Naka, Ibaraki 311-0193 (Japan)

    2013-11-15

    Highlights: • To evaluate the joint fabrication technology for the JT-60SA EF coils, joint resistance measurements were conducted with a joint sample. • The joint sample was composed of pancake and terminal joints. • The measurements demonstrated that both joints fulfilled the design requirement. • Considering the measurements, the characteristics of both joints were investigated using an analytical model that represents the joints. -- Abstract: To evaluate the joint fabrication technology for the JT-60SA EF coils, joint resistance measurements were conducted using a sample consisting of pancake and terminal joints. Both joints are shake-hands lap joints composed of cable-in-conduit conductors and a pure copper saddle-shaped spacer. The measurements demonstrated that both joints fulfilled the design requirement. Considering these measurements, the characteristics of both joints were investigated using analytical models that represent the joints. The analyses indicated that the characteristics of the conductors used in the joints affect the characteristics of the joints.

  4. Safety aspects of the emergency filtration system (EFS) for NRU reactor

    International Nuclear Information System (INIS)

    Hickey, D.L.; Lounsbury, R.I.

    1990-01-01

    A description of the NRU emergency filter system (EFS) is presented, including operation, testing and hazards. Operation of the NRU EFS reduces the dose to the thyroid for members of the public and site personnel by a factor of 100. The calculated thyroid doses for off-site and on-site locations for the current system configuration are 91 and 160 mSv, respectively. An unavailability analysis was performed on the control system which recognized subtle problems in the current system. Modifications to the system result in a system unavailability of 2.0 E-3. The thyroid risk analysis revealed that these improvements in system availability result in risk reduction by approximately a factor of three. Improvements beyond the availability analysis recommendations do not result in substantial risk benefit for members of the public or on-site personnel

  5. Velocity ratio predicts outcomes in patients with low gradient severe aortic stenosis and preserved EF

    DEFF Research Database (Denmark)

    Jander, Nikolaus; Hochholzer, Willibald; Kaufmann, Beat A

    2014-01-01

    OBJECTIVE: To evaluate the usefulness of velocity ratio (VR) in patients with low gradient severe aortic stenosis (LGSAS) and preserved EF. BACKGROUND: LGSAS despite preserved EF represents a clinically challenging entity. Reliance on mean pressure gradient (MPG) may underestimate stenosis severity...... for severe stenosis. We hypothesised that VR may have conceptual advantages over MPG and AVA, predict clinical outcomes and thereby be useful in the management of patients with LGSAS. METHODS: Patients from the prospective Simvastatin and Ezetimibe in Aortic Stenosis (SEAS) study with an AVA...≤40 mm Hg and EF≥55% and asymptomatic at baseline were stratified according to VR with a cut-off value of 0.25. Outcomes were evaluated according to aortic valve-related events and cardiovascular death. RESULTS: Of 435 patients with LGSAS, 197 (45%) had VRVR≥0...

  6. Empréstimo de livros eletrônicos, redes sociais e a proteção de dados dos usuários

    Directory of Open Access Journals (Sweden)

    Liliana Giusti Serra

    2015-01-01

    Full Text Available O artigo discorre sobre a questão da proteção de dados e privacidade dos usuários nas bibliotecas a partir do empréstimo de livros eletrônicos realizado pela parceria OverDrive-Amazon e o uso de redes sociais. É realizado um breve panorama dos normativos relativos à confidencialidade de dados por parte das bibliotecas nos Estados Unidos, países da Europa e a situação brasileira. Finaliza discorrendo sobre a importância da utilização de livros eletrônicos e redes sociais pelas bibliotecas, ressaltando apenas que esse uso seja realizado garantindo a preservação e confidencialidade dos dados pessoais dos usuários, utilizando-se das informações coletadas, mas revertendo-as em ofertas de serviços e divulgação dos acervos.

  7. Single-molecule folding mechanism of an EF-hand neuronal calcium sensor

    DEFF Research Database (Denmark)

    Heiðarsson, Pétur Orri; Otazo, Mariela R.; Bellucci, Luca

    2013-01-01

    EF-hand calcium sensors respond structurally to changes in intracellular Ca2+ concentration, triggering diverse cellular responses and resulting in broad interactomes. Despite impressive advances in decoding their structure-function relationships, the folding mechanism of neuronal calcium sensors...... of the N domain, showing striking interdomain dependence. Molecular dynamics results reveal the atomistic details of the unfolding process and rationalize the different domain stabilities during mechanical unfolding. Through constant-force experiments and hidden Markov model analysis, the free energy...

  8. CARRYING OF FRENCH VISAS FOR HOLDERS OF CATEGORY 'EF' FRENCH ATTESTATIONS DE FONCTIONS

    CERN Multimedia

    Service des Relations avec les Pays Hôtes

    1999-01-01

    The French Ministry of Foreign Affairs has informed the Organisation that members of the personnel resident in Switzerland and holding Category 'EF' French Attestations de functions are not obliged to be in possession of French visas. When temporarily travelling through France on OFFICIAL CERN BUSINESS, provided they are carrying the valid Attestation and a valid national passport with them.Relations with the Host States Servicehttp://www.cern.ch/relations/Tel. 72848

  9. Desenvolvimento de um dispositivo eletrônico para calibração de sensores de umidade do solo Development of eletronic device to calibrate soil moisture sensors

    Directory of Open Access Journals (Sweden)

    Raimundo A. de O. Leão

    2007-04-01

    Full Text Available Com o advento da eletrônica e a disponibilidade de "software" de processamento, vários tipos de transdutores têm sido testados, visando à determinação da umidade do solo. O uso desses transdutores tem por objetivo a otimização do consumo de água e o conseqüente retorno econômico da atividade da agricultura irrigada. No Laboratório de Hidráulica do Departamento de Engenharia Agrícola da Universidade Federal do Ceará, construiu-se um dispositivo para calibração de sensores de umidade do solo, a sua calibração e a determinação de suas principais propriedades, como precisão e cargas mínima e máxima. O dispositivo consistiu numa torre em aço 1020, com 3,0 m de altura, no topo da qual se montou uma balança de braços. Em um dos braços da balança, colocou-se uma amostra de solo para a inserção dos sensores de umidade e, no outro, uma célula de carga para medir a variação de massa de água na amostra de solo. Foi implementado um circuito eletrônico para permitir a interface da célula de carga com um sistema de aquisição de dados. A análise de regressão, cuja variável dependente foi a saída da célula de carga, e a independente, a massa à qual a balança foi submetida, mostrou-se linear entre 1 kg e 50 kg, com coeficiente de determinação de 0,99995 e coeficiente angular de 0,09198 mV g-1. O dispositivo pode ser utilizado para calibração de sensores de umidade do solo com sensibilidade correspondente à variação de 12,28 mL de água e cargas mínima e máxima para as amostras de solo variando entre 1 e 50 kg.Several types of transducers have been tested to measure soil moisture content for irrigation purpose. Their use aims at optimizing water consumption and net profit from irrigation. A research was conducted at the Agricultural Engineering Department at the University Federal of Ceará and aimed at building a device to calibrate soil moisture sensors, in addition to find its major properties, such as

  10. Energy-filtered Photoelectron Emission Microscopy (EF-PEEM) for imaging nanoelectronic materials

    International Nuclear Information System (INIS)

    Renault, Olivier; Chabli, Amal

    2007-01-01

    Photoelectron-Emission Microscopy (PEEM) is the most promising approach to photoemission-based (XPS, UPS) imaging techniques with high lateral resolution, typically below 100 nm. It has now reached its maturity with a new generation of instruments with energy-filtering capabilities. Therefore UPS and XPS imaging with energy-filtered PEEM (EF-PEEM) can be applied to technologically-relevant samples. UPS images with contrast in local work function, obtained with laboratory UV sources, are obtained in ultra-high vacuum environment with lateral resolutions better than 50 nm and sensitivies of 20 meV. XPS images with elemental and bonding state contrast can show up lateral resolution better than 200 nm with synchrotron excitation. In this paper, we present the principles and capabilities of EF-PEEM and nanospectroscopy. Then, we focus on an example of application to non-destructive work-function imaging of polycrystalline copper for advanced interconnects, where it is shown that EF-PEEM is an alternative to Kelvin probes

  11. The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii

    Directory of Open Access Journals (Sweden)

    Sobhan Ghafourian

    2014-07-01

    Full Text Available Although analysis of toxin-antitoxin (TA systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical isolates of A. baumannii and to identify the systems’ locations. For this purpose, we screened 85 A. baumannii isolates collected from different clinical sources for the presence of the mazEF, relBE and higBA TA genes. The results revealed that the genes coding for the mazEF TA system were commonly present in all clinical isolates of A. baumannii. Reverse transcriptase-polymerase chain reaction analysis showed that transcripts were produced in the clinical isolates. Our findings showed that TA genes are prevalent, harboured by chromosomes and transcribed within A. baumannii. Hence, activation of the toxin proteins in the mazEF TA system should be investigated further as an effective antibacterial strategy against this bacterium.

  12. The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii.

    Science.gov (United States)

    Ghafourian, Sobhan; Good, Liam; Sekawi, Zamberi; Hamat, Rukman Awang; Soheili, Sara; Sadeghifard, Nourkhoda; Neela, Vasanthakumari

    2014-07-01

    Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical isolates of A. baumannii and to identify the systems' locations. For this purpose, we screened 85 A. baumannii isolates collected from different clinical sources for the presence of the mazEF, relBE and higBA TA genes. The results revealed that the genes coding for the mazEF TA system were commonly present in all clinical isolates of A. baumannii. Reverse transcriptase-polymerase chain reaction analysis showed that transcripts were produced in the clinical isolates. Our findings showed that TA genes are prevalent, harboured by chromosomes and transcribed within A. baumannii. Hence, activation of the toxin proteins in the mazEF TA system should be investigated further as an effective antibacterial strategy against this bacterium.

  13. TRIO-EF a general thermal hydraulics computer code applied to the Avlis process

    International Nuclear Information System (INIS)

    Magnaud, J.P.; Claveau, M.; Coulon, N.; Yala, P.; Guilbaud, D.; Mejane, A.

    1993-01-01

    TRIO(EF is a general purpose Fluid Mechanics 3D Finite Element Code. The system capabilities cover areas such as steady state or transient, laminar or turbulent, isothermal or temperature dependent fluid flows; it is applicable to the study of coupled thermo-fluid problems involving heat conduction and possibly radiative heat transfer. It has been used to study the thermal behaviour of the AVLIS process separation module. In this process, a linear electron beam impinges the free surface of a uranium ingot, generating a two dimensional curtain emission of vapour from a water-cooled crucible. The energy transferred to the metal causes its partial melting, forming a pool where strong convective motion increases heat transfer towards the crucible. In the upper part of the Separation Module, the internal structures are devoted to two main functions: vapor containment and reflux, irradiation and physical separation. They are subjected to very high temperature levels and heat transfer occurs mainly by radiation. Moreover, special attention has to be paid to electron backscattering. These two major points have been simulated numerically with TRIO-EF and the paper presents and comments the results of such a computation, for each of them. After a brief overview of the computer code, two examples of the TRIO-EF capabilities are given: a crucible thermal hydraulics model, a thermal analysis of the internal structures

  14. Mitos e Realidades sobre a Difusão do Comércio Eletrônico nas Empresas Brasileiras

    Directory of Open Access Journals (Sweden)

    Jason Dedrick

    2003-01-01

    Full Text Available A difusão das tecnologias da informação, e mais recentemente da Internet vem gerando expectativas de transformações radicais na forma de funcionamento da economia mundial. Três hipóteses particularmente importantes pela sua abrangência são destacadas na literatura especializada e serão discutidas neste artigo com base em pesquisas empíricas sobre a difusão do comércio eletrônico conduzidas pelo projeto Globalization and Electronic Commerce, coordenado pela Universidade da Califórnia, Irvine. A primeira é que o processo de difusão do comércio eletrônico está bidirecionalmente associado ao processo de globalização. A segunda é que haveria uma "nova economia" baseada nas novas empresas intensivas em informação. A terceira hipótese analisada é que o comércio eletrônico reduz as desvantagens estruturais das micro e pequenas empresas, graças a seus impactos informacionais e espaciais. Com base em entrevistas com 200 empresas, a pesquisa revela que, pelo menos no caso do Brasil, tais hipóteses não estão se confirmando e que apesar da inegável importância do comércio eletrônico, a tecnologia não determina a estrutura da indústria nem modifica estratégias de globalização. Por outro lado, o artigo confirma que comércio eletrônico é uma importante ferramenta para ajudar empresas e países a desenvolverem trajetórias econômicas preexistentes.The rapid diffusion of information technologies and the Internet gave birth to radical interpretations about their worldwide economic impacts. Three hypotheses widely discussed in the specialized literature are particularly interesting for their relevance for economic analysis and will be reviewed in this article based on empirical research on electronic commerce (EC conducted by the Globalization and Electronic Commerce project at the University of California, Irvine. The first hypothesis states that EC diffusion is both a driving force and a consequence of the

  15. Predictors and Prognostic Value of Worsening Renal Function During Admission in HFpEF Versus HFrEF: Data From the KorAHF (Korean Acute Heart Failure) Registry.

    Science.gov (United States)

    Kang, Jeehoon; Park, Jin Joo; Cho, Young-Jin; Oh, Il-Young; Park, Hyun-Ah; Lee, Sang Eun; Kim, Min-Seok; Cho, Hyun-Jai; Lee, Hae-Young; Choi, Jin Oh; Hwang, Kyung-Kuk; Kim, Kye Hun; Yoo, Byung-Su; Kang, Seok-Min; Baek, Sang Hong; Jeon, Eun-Seok; Kim, Jae-Joong; Cho, Myeong-Chan; Chae, Shung Chull; Oh, Byung-Hee; Choi, Dong-Ju

    2018-03-13

    Worsening renal function (WRF) is associated with adverse outcomes in patients with heart failure. We investigated the predictors and prognostic value of WRF during admission, in patients with preserved ejection fraction (HFpEF) versus those with reduced ejection fraction (HFrEF). A total of 5625 patients were enrolled in the KorAHF (Korean Acute Heart Failure) registry. WRF was defined as an absolute increase in creatinine of ≥0.3 mg/dL. Transient WRF was defined as recovery of creatinine at discharge, whereas persistent WRF was indicated by a nonrecovered creatinine level. HFpEF and HFrEF were defined as a left ventricle ejection fraction ≥50% and ≤40%, respectively. Among the total population, WRF occurred in 3101 patients (55.1%). By heart failure subgroup, WRF occurred more frequently in HFrEF (57.0% versus 51.3%; P failure subgroups. Among various predictors of WRF, chronic renal failure was the strongest predictor. WRF was an independent predictor of adverse in-hospital outcomes (HFrEF: odds ratio; 2.75; 95% confidence interval, 1.50-5.02; P =0.001; HFpEF: odds ratio, 9.48; 95% confidence interval, 1.19-75.89; P =0.034) and 1-year mortality (HFrEF: hazard ratio, 1.41; 95% confidence interval, 1.12-1.78; P =0.004 versus HFpEF: hazard ratio, 1.72; 95% confidence interval, 1.23-2.42; P =0.002). Transient WRF was a risk factor for 1-year mortality, whereas persistent WRF had no additive risk compared to transient WRF. In patients with acute heart failure patients, WRF is an independent predictor of adverse in-hospital and follow-up outcomes in both HFrEF and HFpEF, though with a different effect size. URL: https://www.clinicaltrials.gov. Unique identifier: NCT01389843. © 2018 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

  16. Brief notations on the electronic commerce Breves anotações sobre o comércio eletrônico

    Directory of Open Access Journals (Sweden)

    Sandra Barbon Lewis

    2005-12-01

    new corporation ways of acting born with the online business, merges the necessity of identifying the aspects of the business concentration in this innovative online market.O estudo verifica aspectos da concentração empresarial no comércio eletrônico. Para tanto, primeiramente, é realizada uma análise do papel das empresas no contexto global a partir do avanço tecnológico que é posto à disposição e da nova ordem mundial que lhe é apresentada. O fluxo instantâneo de informações, possibilitado pelo avanço da Internet e o alto grau de competitividade introduzido pela Globalização, levaram os agentes econômicos a buscar no comércio pela Internet uma nova perspectiva de expansão a novos mercados e de comércio internacional. Em seguida passa-se a identificar a formação, surgimento, desenvolvimento e a definição jurídica do comércio eletrônico para trazer à tona suas características e modalidades; observar a estrutura do mercado eletrônico e discutir a passagem da economia convencional a uma nova economia, a economia eletrônica ou digital; questionar a existência de uma empresa eletrônica ou virtual a partir do desenvolvimento da Teoria da Empresa bem como seu modo de atuação na Grande rede. Suplantadas essas contingências iniciais o objetivo foi adentrar no âmbito da concentração empresarial que fomenta o desenvolvimento empresarial, mas também, representa um problema de grandes repercussões econômicas. Sendo assim, buscaram-se, na doutrina, as formas de concentração empresarial e, na Lei antitruste brasileira, a sua disciplina, especificamente o Art. 54 da Lei 8.884/94 que prevê o controle de atos e contratos que podem levar a concentração empresarial. Após a apresentação dos modelos de negócios realizados pelas empresas na Internet, casuisticamente, observou-se a potencialidade de concentração empresarial e as dificuldades de sua caracterização na aplicação da Lei antitruste. Assim, ante os infindáveis recursos

  17. Is the mazEF toxin-antitoxin system responsible for vancomycin resistance in clinical isolates of Enterococcus faecalis?

    Science.gov (United States)

    Sadeghifard, Nourkhoda; Soheili, Sara; Sekawi, Zamberi; Ghafourian, Sobhan

    2014-01-01

    The current study was conducted to investigate the relationship between vancomycin-resistant Enterococcus faecalis (VRE) and the presence of mazEF toxin-antitoxin (TA) system, which may be useful as target for novel antimicrobial therapy concepts. The susceptibility of E. faecalis was determined by MIC, and the presence of the mazEF TA system was evaluated by PCR. Among 200 E. faecalis isolates 39.5% showed resistance to vancomycin (VRE), while 60.5% were susceptible strains (VSE). The mazEF TA system was positive in all VRE isolates (100%), but less prevalent (38/121, 31.4%) among the 121 VSE strains. In conclusion, our study demonstrated a positive relationship between the presence of vancomycin resistance and mazEF TA system. This observation may introduce therapeutic options against a novel antimicrobial target in enterococci.

  18. Proposta de aplicabilidade da preservação digital ao prontuário eletrônico do paciente

    Directory of Open Access Journals (Sweden)

    Virginia Bentes Pinto

    2017-04-01

    Full Text Available Apresentam-se os resultados de uma pesquisa baseada na revisão de literatura sobre a preservação e a curadoria digital, bem como a possibilidade de aplicabilidade ao contexto da documentação sanitária, enquanto memória da saúde e que buscou responder a seguinte questão: Como o padrão Open Archival Information System (OAIS pode ser aplicado aos prontuários eletrônicos do paciente, na perspectiva da preservação de conteúdos digitais, visando assegurar a confidencialidade, confiabilidade, autenticidade e acesso, a quem de direito, à informação registrada nesses documentos? O objetivo básico é estudar a literatura referente à preservação e à curadoria digital, com ênfase no padrão Open Archival Information System (OAIS, considerando sua aplicabilidade ao âmbito dos prontuários eletrônicos de pacientes, com vistas na confidencialidade, confiabilidade, autenticidade e acesso à recuperação da informação, observando-se o ordenamento jurídico concernente a esse documento. Pesquisa exploratória pautada no levantamento do estado da arte sobre o tema em lide. O corpus do estudo foi constituído de 01 prontuário (5 volumes da especialidade da nefrologia, com recorte no ano de 1970. O estudo empírico foi no Serviço de Arquivo Médico e Estatística, do Hospital Universitário Walter Cantídio da Universidade Federal do Ceará. Os achados evidenciam que, embora já existam várias iniciativas sobre a preservação digital da documentação cientifica tecnológica e cultural, não encontramos experiências sobre prontuários. Ademais, o modelo OAIS pode ser aplicado ao contexto do prontuário eletrônico do paciente desde que observadas as características particulares de legalidade de acesso a esses documentos.

  19. Functional role of EF-hands 3 and 4 in membrane-binding of KChIP1

    Indian Academy of Sciences (India)

    The aim of the present study is to explore whether membrane targeting of K+ channel-interacting protein 1 (KChIP1) is associated with its EF-hand motifs and varies with specific phospholipids. Truncated KChIP1, in which the EF-hands 3 and 4 were deleted, retained the -helix structure, indicating that the N-terminal half of ...

  20. Translation elongation factor EF-Tu modulates filament formation of actin-like MreB protein in vitro.

    Science.gov (United States)

    Defeu Soufo, Hervé Joël; Reimold, Christian; Breddermann, Hannes; Mannherz, Hans G; Graumann, Peter L

    2015-04-24

    EF-Tu has been shown to interact with actin-like protein MreB and to affect its localization in Escherichia coli and in Bacillus subtilis cells. We have purified YFP-MreB in an active form, which forms filaments on glass slides in vitro and was active in dynamic light-scattering assays, polymerizing in milliseconds after addition of magnesium. Purified EF-Tu enhanced the amount of MreB filaments, as seen by sedimentation assays, the speed of filament formation and the length of MreB filaments in vitro. EF-Tu had the strongest impact on MreB filaments in a 1:1 ratio, and EF-Tu co-sedimented with MreB filaments, revealing a stoichiometric interaction between both proteins. This was supported by cross-linking assays where 1:1 species were well detectable. When expressed in E. coli cells, B. subtilis MreB formed filaments and induced the formation of co-localizing B. subtilis EF-Tu structures, indicating that MreB can direct the positioning of EF-Tu structures in a heterologous cell system. Fluorescence recovery after photobleaching analysis showed that MreB filaments have a higher turnover in B. subtilis cells than in E. coli cells, indicating different filament kinetics in homologous or heterologous cell systems. The data show that MreB can direct the localization of EF-Tu in vivo, which in turn positively affects the formation and dynamics of MreB filaments. Thus, EF-Tu is a modulator of the activity of a bacterial actin-like protein. Copyright © 2015. Published by Elsevier Ltd.

  1. Development of the finite element method in the thermal field. TRIO-EF software for thermal and radiation analysis

    International Nuclear Information System (INIS)

    Casalotti, N.; Magnaud, J.P.

    1989-01-01

    The possibilities of the TRIO-EF software in the thermal field are presented. The TRIO-EF is a computer program based on the finite element method and used for three-dimensional incompressible flow analysis. It enables the calculation of three-dimensional heat transfer and the fluid/structure analysis. The geometrically complex radiative reactor systems are taken into account in the form factor calculation. The implemented algorithms are described [fr

  2. Kidney-Sparing Methods for Extended-Field Intensity-Modulated Radiotherapy (EF-IMRT) in Cervical Carcinoma Treatment.

    Science.gov (United States)

    Kunogi, Hiroaki; Yamaguchi, Nanae; Terao, Yasuhisa; Sasai, Keisuke

    2016-01-01

    Coplanar extended-field intensity-modulated radiation therapy (EF-IMRT) targeting the whole-pelvic and para-aortic lymph nodes in patients with advanced cervical cancer results in impaired creatinine clearance. An improvement in renal function cannot be expected unless low-dose (approximately 10 Gy) kidney exposure is reduced. The dosimetric method should be considered during EF-IMRT planning to further reduce low-dose exposure to the kidneys. To assess the usefulness of non-coplanar EF-IMRT with kidney-avoiding beams to spare the kidneys during cervical carcinoma treatment in dosimetric analysis between non-coplanar and coplanar EF-IMRT, we compared the doses of the target organ and organs at risk, including the kidney, in 10 consecutive patients. To estimate the influence of EFRT on renal dysfunction, creatinine clearance values after treatment were also examined in 18 consecutive patients. Of these 18 patients, 10 patients who were included in the dosimetric analysis underwent extended field radiation therapy (EFRT) with concurrent chemotherapy, and eight patients underwent whole-pelvis radiation therapy with concurrent chemotherapy to treat cervical carcinoma between April 2012 and March 2015 at our institution. In the dosimetric analysis, non-coplanar EF-IMRT was effective at reducing low-dose (approximately 10 Gy) exposure to the kidneys, thus maintaining target coverage and sparing other organs at risk, such as the small bowel, rectum, and bladder, compared with coplanar EF-IMRT. Renal function in all 10 patients who underwent EFRT, including coplanar EF-IMRT (with kidney irradiation), was low after treatment, and differed significantly from that of the eight patients who underwent WPRT (no kidney irradiation) 6 months after the first day of treatment (P = 0.005). In conclusion, non-coplanar EF-IMRT should be considered in patients with advanced cervical cancer, particularly in patients with a long life expectancy or with pre-existing renal dysfunction.

  3. A neutron scattering study of the ternary complex EF-Tu.GTP-valyl-tRNAVal1A

    DEFF Research Database (Denmark)

    Österberg, R.; Elias, P.; Kjems, Jørgen

    1986-01-01

    The complex formation between elongation factor Tu (EF-Tu), GTP, and valyl-tRNAVal1A has been investigated in a hepes buffer of "pH" 7.4 and 0.2 M ionic strength using the small-angle neutron scattering method at concentrations of D2O where EF-Tu (42% D2O) and tRNA (71% D2O) are successively...

  4. Simultaneous Binding of Multiple EF-Tu Copies to Translating Ribosomes in Live Escherichia coli.

    Science.gov (United States)

    Mustafi, Mainak; Weisshaar, James C

    2018-01-16

    In bacteria, elongation factor Tu is a translational cofactor that forms ternary complexes with aminoacyl-tRNA (aa-tRNA) and GTP. Binding of a ternary complex to one of four flexible L7/L12 units on the ribosome tethers a charged tRNA in close proximity to the ribosomal A site. Two sequential tests for a match between the aa-tRNA anticodon and the current mRNA codon then follow. Because one elongation cycle can occur in as little as 50 ms and the vast majority of aa-tRNA copies are not cognate with the current mRNA codon, this testing must occur rapidly. We present a single-molecule localization and tracking study of fluorescently labeled EF-Tu in live Escherichia coli Imaging at 2 ms/frame distinguishes 60% slowly diffusing EF-Tu copies (assigned as transiently bound to translating ribosome) from 40% rapidly diffusing copies (assigned as a mixture of free ternary complexes and free EF-Tu). Combining these percentages with copy number estimates, we infer that the four L7/L12 sites are essentially saturated with ternary complexes in vivo. The results corroborate an earlier inference that all four sites can simultaneously tether ternary complexes near the A site, creating a high local concentration that may greatly enhance the rate of testing of aa-tRNAs. Our data and a combinatorial argument both suggest that the initial recognition test for a codon-anticodon match occurs in less than 1 to 2 ms per aa-tRNA copy. The results refute a recent study (A. Plochowietz, I. Farrell, Z. Smilansky, B. S. Cooperman, and A. N. Kapanidis, Nucleic Acids Res 45:926-937, 2016, https://doi.org/10.1093/nar/gkw787) of tRNA diffusion in E. coli that inferred that aa-tRNAs arrive at the ribosomal A site as bare monomers, not as ternary complexes. IMPORTANCE Ribosomes catalyze translation of the mRNA codon sequence into the corresponding sequence of amino acids within the nascent polypeptide chain. Polypeptide elongation can be as fast as 50 ms per added amino acid. Each amino acid

  5. Decoding the biosynthesis and function of diphthamide, an enigmatic modification of translation elongation factor 2 (EF2

    Directory of Open Access Journals (Sweden)

    Raffael Schaffrath

    2014-05-01

    Full Text Available Diphthamide is a highly conserved modification of archaeal and eukaryal translation elongation factor 2 (EF2 and yet why cells need EF2 to contain diphthamide is unclear. In yeast, the first steps of diphthamide synthesis and the genes (DPH1-DPH5 required to form the intermediate diphthine are well-documented. However, the last step, amidadation of diphthine to diphthamide, had largely been ill-defined. Remarkably, through mining genome-wide synthetic gene array (SGA and chemical genomics databases, recent studies by Uthman et al. [PLoS Genetics (2013 9, e1003334] and Su et al. [Proc. Natl. Acad. Sci. USA (2012 109, 19983-19987] have identified two more diphthamide players, DPH6 and DPH7. Consistent with roles in the amidation step, dph6 and dph7 deletion strains fail to complete diphthamide synthesis and accumulate diphthine-modified EF2. In contrast to Dph6, the catalytically relevant amidase, Dph7 appears to be regulatory. As shown by Uthman et al., it promotes dissociation of diphthine synthase (Dph5 from EF2, allowing diphthine amidation by Dph6 to occur and thereby coupling diphthine synthesis to the terminal step in the pathway. Remarkably, the study by Uthman et al. suggests that Dph5 has a novel role as an EF2 inhibitor that affects cell growth when diphthamide synthesis is blocked or incomplete and, importantly, shows that diphthamide promotes the accuracy of EF2 performance during translation.

  6. The Influence of the Toxin/Antitoxin mazEF on Growth and Survival of Listeria monocytogenes under Stress.

    Science.gov (United States)

    Curtis, Thomas D; Takeuchi, Ippei; Gram, Lone; Knudsen, Gitte M

    2017-01-13

    A major factor in the resilience of Listeria monocytogenes is the alternative sigma factor B (σ B ). Type II Toxin/Antitoxin (TA) systems are also known to have a role in the bacterial stress response upon activation via the ClpP or Lon proteases. Directly upstream of the σ B operon in L. monocytogenes is the TA system mazEF , which can cleave mRNA at UACMU sites. In this study, we showed that the mazEF TA locus does not affect the level of persister formation during treatment with antibiotics in lethal doses, but exerts different effects according to the sub-inhibitory stress added. Growth of a Δ mazEF mutant was enhanced relative to the wildtype in the presence of sub-inhibitory norfloxacin and at 42 °C, but was decreased when challenged with ampicillin and gentamicin. In contrast to studies in Staphylococcus aureus , we found that the mazEF locus did not affect transcription of genes within the σ B operon, but MazEF effected the expression of the σ B -dependent genes opuCA and lmo0880 , with a 0.22 and 0.05 fold change, respectively, compared to the wildtype under sub-inhibitory norfloxacin conditions. How exactly this system operates remains an open question, however, our data indicates it is not analogous to the system of S. aureus , suggesting a novel mode of action for MazEF in L. monocytogenes.

  7. Designing sequence to control protein function in an EF-hand protein.

    Science.gov (United States)

    Bunick, Christopher G; Nelson, Melanie R; Mangahas, Sheryll; Hunter, Michael J; Sheehan, Jonathan H; Mizoue, Laura S; Bunick, Gerard J; Chazin, Walter J

    2004-05-19

    The extent of conformational change that calcium binding induces in EF-hand proteins is a key biochemical property specifying Ca(2+) sensor versus signal modulator function. To understand how differences in amino acid sequence lead to differences in the response to Ca(2+) binding, comparative analyses of sequence and structures, combined with model building, were used to develop hypotheses about which amino acid residues control Ca(2+)-induced conformational changes. These results were used to generate a first design of calbindomodulin (CBM-1), a calbindin D(9k) re-engineered with 15 mutations to respond to Ca(2+) binding with a conformational change similar to that of calmodulin. The gene for CBM-1 was synthesized, and the protein was expressed and purified. Remarkably, this protein did not exhibit any non-native-like molten globule properties despite the large number of mutations and the nonconservative nature of some of them. Ca(2+)-induced changes in CD intensity and in the binding of the hydrophobic probe, ANS, implied that CBM-1 does undergo Ca(2+) sensorlike conformational changes. The X-ray crystal structure of Ca(2+)-CBM-1 determined at 1.44 A resolution reveals the anticipated increase in hydrophobic surface area relative to the wild-type protein. A nascent calmodulin-like hydrophobic docking surface was also found, though it is occluded by the inter-EF-hand loop. The results from this first calbindomodulin design are discussed in terms of progress toward understanding the relationships between amino acid sequence, protein structure, and protein function for EF-hand CaBPs, as well as the additional mutations for the next CBM design.

  8. Proof of U(VI) sorption on Acidovorax facilis by TRLFS and EF-TEM/EELS

    Energy Technology Data Exchange (ETDEWEB)

    Krawczyk-Baersch, Evelyn; Gerber, Ulrike [Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden (Germany). Div. Biogeochemistry; Steudtner, Robin [Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden (Germany). Div. Surface Processes

    2016-07-01

    In EF-TEM/EELS studies it was shown that U(VI) is sorbed mainly on the outer membrane of Acidovorax facilis. The results are supported by TRLFS measurements, which were performed on the pellet of the cells. In comparison to reference spectra of some cell membrane components, the measured emission spectra of the A. facilis pellet show the best agreement with those of the Uranyl-lipopolysaccharide-complex. Hence, it can be concluded that phosphoryl groups may be the main binding sites for uranyl, located in the lipopolysaccharide unit in the outer membrane.

  9. Estimating Daily Evapotranspiration Based on A Model of Evapotranspiration Fraction (EF) for Mixed Pixels

    Science.gov (United States)

    Xin, X.; Li, F.; Peng, Z.; Qinhuo, L.

    2017-12-01

    Land surface heterogeneities significantly affect the reliability and accuracy of remotely sensed evapotranspiration (ET), and it gets worse for lower resolution data. At the same time, temporal scale extrapolation of the instantaneous latent heat flux (LE) at satellite overpass time to daily ET are crucial for applications of such remote sensing product. The purpose of this paper is to propose a simple but efficient model for estimating daytime evapotranspiration considering heterogeneity of mixed pixels. In order to do so, an equation to calculate evapotranspiration fraction (EF) of mixed pixels was derived based on two key assumptions. Assumption 1: the available energy (AE) of each sub-pixel equals approximately to that of any other sub-pixels in the same mixed pixel within acceptable margin of bias, and as same as the AE of the mixed pixel. It's only for a simpification of the equation, and its uncertainties and resulted errors in estimated ET are very small. Assumption 2: EF of each sub-pixel equals to the EF of the nearest pure pixel(s) of same land cover type. This equation is supposed to be capable of correcting the spatial scale error of the mixed pixels EF and can be used to calculated daily ET with daily AE data.The model was applied to an artificial oasis in the midstream of Heihe River. HJ-1B satellite data were used to estimate the lumped fluxes at the scale of 300 m after resampling the 30-m resolution datasets to 300 m resolution, which was used to carry on the key step of the model. The results before and after correction were compare to each other and validated using site data of eddy-correlation systems. Results indicated that the new model is capable of improving accuracy of daily ET estimation relative to the lumped method. Validations at 12 sites of eddy-correlation systems for 9 days of HJ-1B overpass showed that the R² increased to 0.82 from 0.62; the RMSE decreased to 1.60 MJ/m² from 2.47MJ/m²; the MBE decreased from 1.92 MJ/m² to 1

  10. Função compras no setor público: desafios para o alcance da celeridade dos pregões eletrônicos

    OpenAIRE

    Almeida, Alessandro Anibal Martins de; Sano, Hironobu

    2018-01-01

    Resumo Este artigo aborda a questão dos prazos nos processos de compras no âmbito da administração pública federal. Seu principal objetivo é analisar os fatores que influenciaram na execução das subfases do pregão eletrônico na Base Aérea de Natal (Bant), tendo como parâmetro o princípio da celeridade. Baseado na teoria sobre a função compras, quatro dimensões foram estabelecidas: “Legislação e processos de compras”, “Estrutura e organização do setor de compras”, “Sistemas de compras” e “Recu...

  11. Um modelo semântico de publicações eletrônicas | A semantic model for electronic publishing

    Directory of Open Access Journals (Sweden)

    Carlos Henrique Marcondes

    2011-03-01

    Full Text Available Resumo Publicações eletrônicas, apesar dos avanços das Tecnologias da Informação, são ainda calcados no modelo impresso. O formato textual impede que programas possam ser usados para o processamento “semântico” desses conteúdos. È porposto um modelo “semântico” de publicações cientificas eletrônicas, no qual as conclusões contidas no texto do artigo fornecidas por autores e representadas em formato “inteligível” por programas, permitindo recuperação semântica, identificação de indícios de novas descobertas científicas e de incoerências sobre este conhecimento. O modelo se baseia nos conceitos de estrutura profunda, ou semântica, da linguagem (CHOMSKY, 1975, de microestrutura, macroestrutura e superestrutura, (KINTSH, VAN DIJK, 1972, na estrutura retórica de artigos científicos (HUTCHINS, 1977, (GROSS, 1990 e nos elementos de metodologia cientifica, como problema, questão, objetivo, hipótese, experimento e conclusão. Resulta da análise de 89 artigos biomédicos. Foi desenvolvido um protótipo de sistema que implementa parcialmente o modelo. Questionários foram usados com autores para embasar o desenvolvimento do protótipo. O protótipo foi testando com pesquisadores-autores. Foram identificados quatro padrões de raciocínio e encadeamento dos elementos semânticos em artigos científicos. O modelo de conteúdo foi implementado como uma ontologia computacional. Foi desenvolvido e avaliado um protótipo de uma interface web de submissão artigos pelos autores a um sistema eletrônico de publicação de periódicos que implementa o modelo. Palavras-chave publicações eletrônicas; metodológica científica; comunicação científica; representação do conhecimento; ontologias; processamento semântico de conteúdos; e-Ciência Abstract Electronic publishing, although Information Technologies advancements, are still based in the print text model. The textual format prevents programs to semantic process

  12. Lino: game eletrônico para auxiliar na educação ambiental de crianças em idade escolar

    Directory of Open Access Journals (Sweden)

    Aldo Nascimento Pontes

    2017-12-01

    Full Text Available O caráter lúdico dos games, nas últimas décadas, tem mostrado seu rico potencial na dinamização de processos de ensino-aprendizagem. Este trabalho tem por objetivo apresentar os resultados de uma pesquisa que desenvolveu um game eletrônico para auxiliar na Educação Ambiental de crianças em idade escolar. Assim, por meio de uma pesquisa experimental, desenvolveu-se e implementou-se o jogo Lino, o qual foi submetido a uma avaliação de funcionalidades.  Os resultados mostraram que o Lino representa um caminho lúdico, agradável e didático para expandir a conscientização e a atitude das crianças sobre a questão ambiental.

  13. Dependência de Internet e de jogos eletrônicos: uma revisão Internet and videogame addiction: a review

    Directory of Open Access Journals (Sweden)

    Cristiano Nabuco de Abreu

    2008-06-01

    Full Text Available OBJETIVO: Com a inclusão das novas tecnologias contemporâneas, a Internet e os jogos eletrônicos tornaram-se ferramentas de uso amplo e irrestrito, transformando-se em um dos maiores fenômenos mundiais da última década. Diversas pesquisas atestam os benefícios desses recursos, mas seu uso sadio e adaptativo progressivamente deu lugar ao abuso e à falta de controle ao criar severos impactos na vida cotidiana de milhões de usuários. O objetivo deste estudo foi revisar de forma sistemática os artigos que examinam a dependência de Internet e jogos eletrônicos na população geral. Almejamos, portanto, avaliar a evolução destes conceitos no decorrer da última década, assim como contribuir para a melhor compreensão do quadro e suas comorbidades. MÉTODO: Foi feita uma revisão sistemática da literatura através do MedLine, Lilacs, SciELO e Cochrane usando-se como parâmetro os termos: "Internet addiction", pathological "Internet use", "problematic Internet use", "Internet abuse", "videogame", "computer games" e "electronic games". A busca eletrônica foi feita até dezembro de 2007. DISCUSSÃO: Estudos realizados em diferentes países apontam para prevalências ainda muito diversas, o que provavelmente se deve à falta de consenso e ao uso de diferentes denominações, dando margem à adoção de distintos critérios diagnósticos. Muitos pacientes que relatam o uso abusivo e dependência passam a apresentar prejuízos significativos na vida profissional, acadêmica (escolar, social e familiar. CONCLUSÕES: São necessárias novas investigações para determinar se esse uso abusivo de Internet e de jogos eletrônicos pode ser compreendido como uma das mais novas classificações psiquiátricas do século XXI ou apenas substratos de outros transtornos.OBJECTIVE: With the introduction of the new technologies to our daily lives, the Internet and electronic games have become widely and unrestrictedly used tools and one of the major

  14. Avaliação do uso de identificadores eletrônicos em suínos Evaluating the use of electronic identification in swine

    Directory of Open Access Journals (Sweden)

    Késia O. da Silva

    2006-04-01

    Full Text Available O mercado consumidor, atualmente, exige aumento gradativo do controle da qualidade dos produtos. Os métodos manuais de controle existentes, aplicáveis à produção animal, começam a se mostrar ineficientes para garantir percentual crescente dessa qualidade, pois essa garantia somente pode ser efetiva se houver rastreamento confiável do animal, desde o seu nascimento até o abate. A identificação individual apresenta grande importância nesse enfoque, pois possibilita a coleta de informações inerentes ao animal. A rastreabilidade eletrônica utiliza dispositivo eletrônico, que emite um sinal ativado por um leitor fixo, colocado onde for necessário registrar um determinado evento, ou um leitor manual que permite maior independência do operador. Sabendo-se da importância da identificação eletrônica como ferramenta para a rastreabilidade dos animais produzidos comercialmente, teve-se o objetivo de estudar o uso do identificador eletrônico (transponder, de forma a garantir tanto a leitura manual do indivíduo, como a leitura com antena fixa. Foram implantados nos seguintes locais: 1 fronte; 2 lóbulo externo; 3 parte posterior da base auricular, e 4 dentro de um brinco e implantado no lóbulo externo da orelha. Foram analisados os fatores seqüela e migração, posterior ao implante, assim como a eficiência de leitura na antena tanto fixa como manual. O melhor local de implante foi a parte posterior da base auricular.Nowadays the consumer market demands a gradually increase in the products' quality control. The manual control that exits, used in animal production, shows ineficiency in warrating an increasing percentual of the desirable quality, so this can only be reached when an effective animal tracebility system is applied, from birth to slaughter. Individual electronic identification presents high importance in this focus, providing information recorded directly from the animal. Electronic traceability uses electronic devices

  15. Governo Eletrônico: abordagem multicritério para priorização de projetos integrando indicadores da Metodologia BCS

    OpenAIRE

    CANEDO, Mônica Maria Leal

    2008-01-01

    A melhoria do serviço público, não só do atendimento ao cidadão, mas também dos resultados alcançados pela implementação das Políticas Públicas, é um grande desafio que se apresenta a gestores de todas as esferas de Governo. O Governo Eletrônico representa um compromisso de Gestão com o uso intensivo da Tecnologia da Informação (TI), visando ao incremento da efetividade e da transparência das ações do Governo, mudando paradigmas de relacionamento de uma estrutura de comando-con...

  16. Informação Pública e Governo Eletrônico na Assembleia Legislativa da Bahia │ Public information and electronic government

    Directory of Open Access Journals (Sweden)

    José Carlos Sales dos Santos

    2013-11-01

    Full Text Available Resumo Com o potencial de interatividade e compartilhamento de conteúdos em rede, os governos viabilizam condições diversas de acesso a informações governamentais, prerrogativa essencial para a consolidação da transparência política no processo democrático. Neste contexto, e compreendida como órgão governamental produtor de informações públicas, a Assembleia Legislativa da Bahia – ALBA também procura disponibilizar conteúdos digitais orientados à sociedade. O artigo do presente artigo é analisar o modus operandi dos dispositivos comunicacionais utilizados para a disseminação e consecução de conteúdos informacionais públicos através do website da ALBA, engendrada nas estruturas de prestação de serviços propostas pelo Governo Eletrônico. Discutem-se os aspectos conceituais do governo eletrônico, as perspectivas e propostas focalizadas no marco legal brasileiro, para apresentar as possíveis aproximações da Assembleia com as categorias previstas no governo eletrônico. O roteiro de entrevista orientado aos responsáveis administrativos do website da ALBA, e a observação sistematizada do sítio, constituíram os principais instrumentos de coleta de dados. A conclusão evidencia que o site analisado respondeu satisfatoriamente à disponibilidade de informações relacionadas aos parlamentares, mas, no plano ‘interatividade’, a pesquisa apresentou uma relativa ineficácia administrativa para responder às demandas dos cidadãos. Palavras-chave Assembleia Legislativa da Bahia – ALBA, Governo Eletrônico, Conteúdos Informacionais Digitais, Informação Pública. Abstract As a governmental producer of public information, the Assembleia Legislativa da Bahia – ALBA offers political oriented digital content to citizens, as a prerogative to transparency of government activities. These informational digital content require consistent with the interest of the public and the audience to meet the essential conditions

  17. Nordestinidade: identidade e machismo no forró pé de serra e no forró eletrônico

    OpenAIRE

    Verunschk, Micheliny

    2015-01-01

    Resumo:Em No Ceará não tem disso não: nordestinidade e macheza no forró contemporâneo, Felipe Trotta analisa a construção da identidade nordestina e o manejo dos estereótipos genéricos nos discursos do forró tradicional e do forró eletrônico na construção. Abstract:In No Ceará não tem disso não: nordestinidade e macheza no forró contemporâneo, Felipe Trotta analyses the discourse of traditional forró music and electronic forró music, and the demands for the maintenance/rebuilding of northe...

  18. Levantamento de dados sobre a reciclagem do lixo eletrônico no município de Medianeira – PR

    Directory of Open Access Journals (Sweden)

    Patrícia de Abreu Bueno

    2013-01-01

    Full Text Available Este trabalho teve como temática a gestão dos resíduos eletrônicos no município de Medianeira-PR, tendo por objetivos abordar e verificar se as empresas prestadoras de serviço da área de informática e eletrônica instaladas no município de Medianeira estão destinando corretamente seus resíduos, se a população tem conhecimento sobre este resíduo bem como se possui acesso a postos de coleta para realizar a destinação do lixo eletrônico. Os dados analisados foram coletados por meio de entrevistas e questionário aplicado com a população e com as empresas instaladas no município. Constatou-se que apesar das empresas terem preocupação com a destinação dos resíduos e direcionar os mesmos para empresa responsável por separar e processar todo o lixo coletado, a população possui pouca informação referente à correta destinação, muito se tem a melhorar, no que diz respeito à criação de instrumentos que regulamentem o problema dos resíduos eletrônicos no município e o desenvolvimento de campanhas e programas à população, para que a mesma direcione esses resíduos aos pontos de coleta a fim de assegurar o melhor aproveitamento deste lixo, para minimizar oscustos do poder público com a disposição final e possibilitar a melhoria das condições ambientais e da qualidade de vida dos munícipes.Abstract This work had as its theme the management of electronic waste in the municipality of Medianeira-PR and aimed to approach and verify if computer science and electronics companies installed in the municipality of Medianeira have been allocating their e-waste correctly, whether the population is aware about this residue as well to have access to collection points to make the fair allocation of this sort of junk. Data were collected through interviews and questionnaire applied to the population and enterprisesthroughout the municipality. It was found that although companies are concerned about waste disposal and direct

  19. Desenvolvimento de um penetrômetro manual eletrônico - doi: 10.4025/actascitechnol.v33i1.5914

    Directory of Open Access Journals (Sweden)

    Rafael Cesar Tieppo

    2011-03-01

    Full Text Available O excesso de tráfego de máquinas pode causar compactação prejudicial ao desenvolvimento da cultura. Uma das maneiras de se obter um indicativo de compactação é a resistência do solo à penetração (RSP. Assim, desenvolveu-se um penetrômetro eletrônico, seguindo a norma S313.2 ASAE (1998, tendo a haste 0,0095 m de diâmetro e o cone de 30° com 0,01283 m de diâmetro na base. Para o desenvolvimento do penetrômetro, utilizou-se um sistema para aquisição de dados, sensor de distância, sensor de força e barras circulares de aço inox para confecção da haste e do cone. O aparelho armazena os dados coletados a campo em uma frequência de 4 Hz, sendo estes posteriormente transferidos para um microcomputador, via interface RS232. Por meio de uma planilha eletrônica, os dados podem ser processados de acordo com a necessidade do usuário. Os sensores apresentaram confiabilidade em sua utilização, os arquivos de dados gerados pelo aparelho proporcionaram conforto e confiança no processamento de dados e os dados puderam ser reorganizados conforme a necessidade do usuário para, assim, evitar o erro de leitura pelo processo visual.

  20. Consumidor de Dispositivos Eletrônicos Portáteis: O Efeito Moderador do Perfil do Adotante na Intenção de Compra

    Directory of Open Access Journals (Sweden)

    Washington Romão dos Santos

    2016-12-01

    Full Text Available Considerando o crescimento constante observado nos últimos anos dos dispositivos eletrônicos enquanto facilitadores de transações comerciais, sobretudo no segmento B2C, com o objetivo de melhor compreender as relações entre a percepção de valor e a efetiva aquisição de um produto ou serviço pelo consumidor, este paper apresenta os resultados de um estudo que explora o perfil do adotante de inovações como possível moderador na relação entre o Valor Percebido e a Intenção de Compra de produtos eletrônicos portáteis (smartphones, tablets, ultrabooks e leitores de e-books. Trata-se de uma pesquisa quantitativa com uma amostra de 350 respondentes coletada por snowball sampling no Facebook e posteriormente avaliada por meio da Modelagem de Equações Estruturais (SEM. Os resultados confirmam a relação do Valor Percebido na Intenção de Compra além de sustentar a influência moderadora do Perfil do Adotante sobre essa relação. Dentre as variáveis antecedentes relacionadas à adoção de tecnologia, vale destacar que a Facilidade de Uso Percebida, a Complexidade Percebida e o Risco Percebido não apresentaram efeito significativo sobre o Valor Percebido. Por fim, os resultados da pesquisa indicam que as organizações devem procurar desenvolver ações de acordo com os diferentes perfis de consumidor. 

  1. Mammalian translation elongation factor eEF1A2: X-ray structure and new features of GDP/GTP exchange mechanism in higher eukaryotes.

    Science.gov (United States)

    Crepin, Thibaut; Shalak, Vyacheslav F; Yaremchuk, Anna D; Vlasenko, Dmytro O; McCarthy, Andrew; Negrutskii, Boris S; Tukalo, Michail A; El'skaya, Anna V

    2014-11-10

    Eukaryotic elongation factor eEF1A transits between the GTP- and GDP-bound conformations during the ribosomal polypeptide chain elongation. eEF1A*GTP establishes a complex with the aminoacyl-tRNA in the A site of the 80S ribosome. Correct codon-anticodon recognition triggers GTP hydrolysis, with subsequent dissociation of eEF1A*GDP from the ribosome. The structures of both the 'GTP'- and 'GDP'-bound conformations of eEF1A are unknown. Thus, the eEF1A-related ribosomal mechanisms were anticipated only by analogy with the bacterial homolog EF-Tu. Here, we report the first crystal structure of the mammalian eEF1A2*GDP complex which indicates major differences in the organization of the nucleotide-binding domain and intramolecular movements of eEF1A compared to EF-Tu. Our results explain the nucleotide exchange mechanism in the mammalian eEF1A and suggest that the first step of eEF1A*GDP dissociation from the 80S ribosome is the rotation of the nucleotide-binding domain observed after GTP hydrolysis. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

  2. Arabidopsis EF-Tu receptor enhances bacterial disease resistance in transgenic wheat.

    Science.gov (United States)

    Schoonbeek, Henk-Jan; Wang, Hsi-Hua; Stefanato, Francesca L; Craze, Melanie; Bowden, Sarah; Wallington, Emma; Zipfel, Cyril; Ridout, Christopher J

    2015-04-01

    Perception of pathogen (or microbe)-associated molecular patterns (PAMPs/MAMPs) by pattern recognition receptors (PRRs) is a key component of plant innate immunity. The Arabidopsis PRR EF-Tu receptor (EFR) recognizes the bacterial PAMP elongation factor Tu (EF-Tu) and its derived peptide elf18. Previous work revealed that transgenic expression of AtEFR in Solanaceae confers elf18 responsiveness and broad-spectrum bacterial disease resistance. In this study, we developed a set of bioassays to study the activation of PAMP-triggered immunity (PTI) in wheat. We generated transgenic wheat (Triticum aestivum) plants expressing AtEFR driven by the constitutive rice actin promoter and tested their response to elf18. We show that transgenic expression of AtEFR in wheat confers recognition of elf18, as measured by the induction of immune marker genes and callose deposition. When challenged with the cereal bacterial pathogen Pseudomonas syringae pv. oryzae, transgenic EFR wheat lines had reduced lesion size and bacterial multiplication. These results demonstrate that AtEFR can be transferred successfully from dicot to monocot species, further revealing that immune signalling pathways are conserved across these distant phyla. As novel PRRs are identified, their transfer between plant families represents a useful strategy for enhancing resistance to pathogens in crops. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

  3. Energy consumption: energy consumption in mainland Norway

    Energy Technology Data Exchange (ETDEWEB)

    Magnussen, Inger Helene; Killingland, Magnus; Spilde, Dag

    2012-07-25

    The purpose of this report is to describe trends in energy consumption in mainland Norway, with an emphasis on key trends within the largest consumer groups. We also explain common terms and concepts in the field of energy consumption. Finally, we look at forecasts for future energy consumption, produced by bodies outside NVE. Total final energy consumption in mainland Norway in 2009 was 207 TWh. The most important end-user groups are households, service industries, manufacturing industry and transport. In addition, the energy sector in mainland Norway consumed 15 TWh. Energy consumed in the energy sector is not considered as final consumption, as the energy is used to produce new energy products. The long-term trend in energy consumption in mainland Norway is that fuel in the transport sector and electricity for the energy sector increases, while energy consumption in other sectors flattens out. The main reason for an increased use of fuel in the transport sector is the rise in the number of motorised machinery and vehicles in mainland Norway. This has caused a rise in gasoline and diesel consumption of 75 per cent since 1976. The petroleum sector is the largest consumer of energy within the energy sector in mainland Norway, and electricity from onshore to platforms in the North Sea and to new shore side installations has led to a rise in electricity consumption from 1 TWh in 1995 to 5 TWh in 2009. The energy consumption in households showed flat trend from 1996 to 2009, after many years of growth. The main reasons are a warmer climate, higher energy prices, the use of heats pumps and more energy-efficient buildings. In the service industries, the growth in energy consumptions has slightly decreased since the late 1990s, for much the same reasons as for households. In manufacturing industries the energy consumption have flatten out mainly due to the closure of energy-intensive businesses and the establishment of new more energy-efficient businesses. Electricity is

  4. Translation elongation factor eEF1A2 is a potential oncoprotein that is overexpressed in two-thirds of breast tumours

    Directory of Open Access Journals (Sweden)

    Miller William R

    2005-09-01

    Full Text Available Abstract Background The tissue-specific translation elongation factor eEF1A2 was recently shown to be a potential oncogene that is overexpressed in ovarian cancer. Although there is no direct evidence for an involvement of eEF1A2 in breast cancer, the genomic region to which EEF1A2 maps, 20q13, is frequently amplified in breast tumours. We therefore sought to establish whether eEF1A2 expression might be upregulated in breast cancer. Methods eEF1A2 is highly similar (98% to the near-ubiquitously expressed eEF1A1 (formerly known as EF1-α making analysis with commercial antibodies difficult. We have developed specific anti-eEF1A2 antibodies and used them in immunohistochemical analyses of tumour samples. We report the novel finding that although eEF1A2 is barely detectable in normal breast it is moderately to strongly expressed in two-thirds of breast tumours. This overexpression is strongly associated with estrogen receptor positivity. Conclusion eEF1A2 should be considered as a putative oncogene in breast cancer that may be a useful diagnostic marker and therapeutic target for a high proportion of breast tumours. The oncogenicity of eEF1A2 may be related to its role in protein synthesis or to its potential non-canonical functions in cytoskeletal remodelling or apoptosis.

  5. Translation elongation factor eEF1A2 is a potential oncoprotein that is overexpressed in two-thirds of breast tumours

    International Nuclear Information System (INIS)

    Tomlinson, Victoria AL; Newbery, Helen J; Wray, Naomi R; Jackson, Juliette; Larionov, Alexey; Miller, William R; Dixon, J Michael; Abbott, Catherine M

    2005-01-01

    The tissue-specific translation elongation factor eEF1A2 was recently shown to be a potential oncogene that is overexpressed in ovarian cancer. Although there is no direct evidence for an involvement of eEF1A2 in breast cancer, the genomic region to which EEF1A2 maps, 20q13, is frequently amplified in breast tumours. We therefore sought to establish whether eEF1A2 expression might be upregulated in breast cancer. eEF1A2 is highly similar (98%) to the near-ubiquitously expressed eEF1A1 (formerly known as EF1-α) making analysis with commercial antibodies difficult. We have developed specific anti-eEF1A2 antibodies and used them in immunohistochemical analyses of tumour samples. We report the novel finding that although eEF1A2 is barely detectable in normal breast it is moderately to strongly expressed in two-thirds of breast tumours. This overexpression is strongly associated with estrogen receptor positivity. eEF1A2 should be considered as a putative oncogene in breast cancer that may be a useful diagnostic marker and therapeutic target for a high proportion of breast tumours. The oncogenicity of eEF1A2 may be related to its role in protein synthesis or to its potential non-canonical functions in cytoskeletal remodelling or apoptosis

  6. Fluctuations between multiple EF-G-induced chimeric tRNA states during translocation on the ribosome

    Science.gov (United States)

    Adio, Sarah; Senyushkina, Tamara; Peske, Frank; Fischer, Niels; Wintermeyer, Wolfgang; Rodnina, Marina V.

    2015-06-01

    The coupled translocation of transfer RNA and messenger RNA through the ribosome entails large-scale structural rearrangements, including step-wise movements of the tRNAs. Recent structural work has visualized intermediates of translocation induced by elongation factor G (EF-G) with tRNAs trapped in chimeric states with respect to 30S and 50S ribosomal subunits. The functional role of the chimeric states is not known. Here we follow the formation of translocation intermediates by single-molecule fluorescence resonance energy transfer. Using EF-G mutants, a non-hydrolysable GTP analogue, and fusidic acid, we interfere with either translocation or EF-G release from the ribosome and identify several rapidly interconverting chimeric tRNA states on the reaction pathway. EF-G engagement prevents backward transitions early in translocation and increases the fraction of ribosomes that rapidly fluctuate between hybrid, chimeric and posttranslocation states. Thus, the engagement of EF-G alters the energetics of translocation towards a flat energy landscape, thereby promoting forward tRNA movement.

  7. The SOS response is permitted in Escherichia coli strains deficient in the expression of the mazEF pathway.

    Science.gov (United States)

    Kalderon, Ziva; Kumar, Sathish; Engelberg-Kulka, Hanna

    2014-01-01

    The Escherichia coli (E. coli) SOS response is the largest, most complex, and best characterized bacterial network induced by DNA damage. It is controlled by a complex network involving the RecA and LexA proteins. We have previously shown that the SOS response to DNA damage is inhibited by various elements involved in the expression of the E. coli toxin-antitoxin mazEF pathway. Since the mazEF module is present on the chromosomes of most E. coli strains, here we asked: Why is the SOS response found in so many E. coli strains? Is the mazEF module present but inactive in those strains? We examined three E. coli strains used for studies of the SOS response, strains AB1932, BW25113, and MG1655. We found that each of these strains is either missing or inhibiting one of several elements involved in the expression of the mazEF-mediated death pathway. Thus, the SOS response only takes place in E. coli cells in which one or more elements of the E. coli toxin-antitoxin module mazEF or its downstream pathway is not functioning.

  8. A comparison of EF-18 agar and modified brilliant green agar with lutensit for isolation of Salmonella from poultry samples

    DEFF Research Database (Denmark)

    Petersen, Line Hedegård

    1997-01-01

    -Vassiliadis broth, and 3) Plating onto EF-18 agar and BGA/L simultaneously. From a total of 1101 samples, Salmonella was isolated from 158, 157 of which were faecal samples. Thirtyone of these isolates were recovered on one medium only, 18 could not be found on BGA/L and 13 could not be found on EF-18 agar....... The relative specificity and sensitivity of each plating agar was determined by enumeration of false-positive and false-negative reactions. EF-18 agar compared favourably with BGA/L, displaying a sensitivity of 0.92 as opposed to 0.89 for BGA/L, calculated for the ''fecal samples'' group only. The calculated...... specificities for each group of samples were likewise considerably higher for EF-18 agar (0.75-0.91) than for BGA/L (0.35-0.55). Though EF-18 agar is slightly more expensive than BGA/L, the routine use of the former may result in a considerable reduction in overall laboratory costs due to its superior...

  9. Desenvolvimento de circuito eletrônico comparador para controle de válvulas de vazões reduzidas = Development of comparative electronic circuit for reduced flow valve control

    Directory of Open Access Journals (Sweden)

    Ana Maria Frattini Fileti

    2011-04-01

    Full Text Available Diversos tipos de válvula de controle de vazão estão disponíveiscomercialmente, sendo a maioria delas apropriada para elevadas vazões e provida de acionamento pneumático para reduzir riscos de explosões em ambientes industriais. As válvulas para vazões reduzidas e baixas pressões são de pequenas dimensões, normalmente eletrônicas e apresentam custo elevado. No presente trabalho foi desenvolvido um sistema de controle eletrônico de vazão por computador on-line, baseado em componentes eletrônicos comuns, em esquema comparativo de tensão. Um amplificador subtrador foi utilizado neste circuito eletrônico, comparando dois valores de tensão para o controle dosentido de rotação e parada do motorredutor de velocidade acoplado à haste da válvula agulha comum. Este sistema de baixo custo se mostrou eficiente e de simples montagem eletrônica. Válvulas de diferentes dimensões foram acopladas com sucesso ao motorredutor comandado pelo circuito eletrônico, conferindo flexibilidade ao atuador desenvolvido.Ensaios de calibração e de determinação de tempo morto são apresentados, obtendo-se como menor faixa de vazão os valores de 0,3 a 3,6 mL s-1.Different types of flow control valves are commercially available but most are only adequate for high flow rates. These are provided with pneumatic devices to reduce explosion risks in industrial plants. Flow-reduced and low pressure valves are small sized, usually driven by analogical signals and very expensive. Current assay developed an on-line computer-aided flow control electronic system made up of common electronic components within a comparative voltage scheme. A subtracted amplifier was employed in the electronic circuit which compares two voltages for rotation direction control and stoppage of speed engine reducer linked to the common valve stem. This low cost system is very efficient and has the advantage of being electronically assembled without any difficulty. Different

  10. Comparação das formas de aplicação papel e caneta e eletrônica do índice de estresse parental versão reduzida (PSI-SF

    Directory of Open Access Journals (Sweden)

    Camila Piccini Aiello

    2014-04-01

    Full Text Available Objetivo avaliar se a aplicação eletrônica do Índice de Estresse Parental – versão reduzida (PSI-SF é comparável à aplicação em formato papel e caneta. Verificar o estresse em pais de crianças com desenvolvimento normal. Métodos quarenta adultos, pais de crianças entre seis meses e 10 anos, foram divididos em quatro grupos, sendo pareados por idade, sexo, escolaridade e idade da criança. Cada participante completou o questionário em duas ocasiões, com intervalo de sete a dez dias, nas versões: papel-caneta/papel-caneta (PP, papel-caneta/eletrônico (PE, eletrônico/eletrônico (EE, eletrônico/papel-caneta (EP. O PSI-SF apresenta 36 afirmações, divididas em três subescalas: Sofrimento Parental (SP; Interações Disfuncionais entre Pai e Criança (IDPC e Criança Difícil (CD. Para análise dos dados foram realizadas as correlações (Pearson e comparação da pontuação do PSI-SF intra e inter-grupos. Resultados foram obtidas correlações positivas fortes e médias e significantes entre a pontuação total e das subescalas do PSI-SF na primeira e segunda aplicação, para todos os grupos. Diferenças significantes foram observadas entre as médias das pontuações para o grupo PP (subescala “Criança Difícil”; e grupo EE (subescala “Criança Difícil”; e pontuação total. No entanto, tais diferenças de pontuação não alteraram a interpretação do resultado do questionário. Não houve diferença significante entre os grupos para as quatro subescalas analisadas, confirmando equivalência da variância entre os grupos. O estresse dos participantes, em todas as subescalas, recaiu dentro da normalidade. Conclusão a aplicação no formato eletrônico do questionário PSI-SF apresenta resultados semelhantes à aplicação papel e caneta. Os níveis de estresse observados foram considerados normais.

  11. High performance message passing for the ATLAS DAQ/EF-1 project

    CERN Document Server

    Mornacchi, Giuseppe

    1999-01-01

    Summary form only. A message passing library has been developed in the context of the ATLAS DAQ/EF-1 project. It is used for time critical applications within the front-end part of the DAQ system, mainly to exchange data control messages between I/O processors. Key objectives of the design were low message overheads, efficient use of the data transfer buses, provision of broadcast functionality and a hardware and operating system independent implementation of the application interface. The design and implementation of the message passing library are presented. As required by the project, the implementation is based on commercial components, namely VMEbus, PCI, the Lynx-OS real-time operating system and an additional inter- processor link, PVIC. The latter offers broadcast functionality identified as being important to the overall performance of the message passing. In addition, performance benchmarks for all implementing buses are presented for both simple test programs and the full DAQ applications. (0 refs)...

  12. Orbital period variations of two W UMa-type binaries: UY UMa and EF Boo

    Science.gov (United States)

    Yu, Yun-Xia; Zhang, Xu-Dong; Hu, Ke; Xiang, Fu-Yuan

    2017-08-01

    The orbital period variations of two W UMa-type contact binaries, UY UMa and EF Boo, are analyzed by using all available times of light minimum. It is detected that the general trends of their (O - C) curves show an upward parabolic variation, which reveals their continuous period increases at the rates of dP / dt = 2.545 ×10-7 days yr-1 and dP / dt = 2.623 ×10-7 days yr-1 , respectively. Meanwhile, UY UMa also shows a cyclic period variation with a small amplitude of A = 0.0026 days superposed on the long-term increase. Due to their contact configurations, the secular period increases are interpreted as a result of mass transfer from the less massive component to the more massive one. The cyclic period variation of UY UMa may be interpreted in terms of either the magnetic activity or the light time effect.

  13. Ef: Software for Nonrelativistic Beam Simulation by Particle-in-Cell Algorithm

    Directory of Open Access Journals (Sweden)

    Boytsov A. Yu.

    2018-01-01

    Full Text Available Understanding of particle dynamics is crucial in construction of electron guns, ion sources and other types of nonrelativistic beam devices. Apart from external guiding and focusing systems, a prominent role in evolution of such low-energy beams is played by particle-particle interaction. Numerical simulations taking into account these effects are typically accomplished by a well-known particle-in-cell method. In practice, for convenient work a simulation program should not only implement this method, but also support parallelization, provide integration with CAD systems and allow access to details of the simulation algorithm. To address the formulated requirements, development of a new open source code - Ef - has been started. It's current features and main functionality are presented. Comparison with several analytical models demonstrates good agreement between the numerical results and the theory. Further development plans are discussed.

  14. Ef: Software for Nonrelativistic Beam Simulation by Particle-in-Cell Algorithm

    Science.gov (United States)

    Boytsov, A. Yu.; Bulychev, A. A.

    2018-04-01

    Understanding of particle dynamics is crucial in construction of electron guns, ion sources and other types of nonrelativistic beam devices. Apart from external guiding and focusing systems, a prominent role in evolution of such low-energy beams is played by particle-particle interaction. Numerical simulations taking into account these effects are typically accomplished by a well-known particle-in-cell method. In practice, for convenient work a simulation program should not only implement this method, but also support parallelization, provide integration with CAD systems and allow access to details of the simulation algorithm. To address the formulated requirements, development of a new open source code - Ef - has been started. It's current features and main functionality are presented. Comparison with several analytical models demonstrates good agreement between the numerical results and the theory. Further development plans are discussed.

  15. Gestão do processo de design de arquitetura efêmera em museus

    OpenAIRE

    Veiga, Ana Cecilia Nascimento Rocha; Andery, Paulo Roberto Pereira

    2014-01-01

    A gestão de empreendimentos de museus e espaços museográficos têm crescido em complexidade, particularmente na sua fase de concepção. Contribui para isso o reduzido ciclo de vida dos empreendimentos, a arquitetura efêmera, que supõe pouco tempo para projetação, execução e montagem. Soma-se a mudança de significado que os espaços museográficos tiveram em anos recentes, com a interação entre acervo, ambiente construído e público, gerando um briefing mais dinâmico e complexo. Nesse contexto, o p...

  16. The Influence of the Toxin/Antitoxin mazEF on Growth and Survival of Listeria monocytogenes under Stress

    DEFF Research Database (Denmark)

    Curtis, Thomas; Takeuchi, Ippei; Gram, Lone

    2017-01-01

    A major factor in the resilience of Listeria monocytogenes is the alternative sigma factor B (σB). Type II Toxin/Antitoxin (TA) systems are also known to have a role in the bacterial stress response upon activation via the ClpP or Lon proteases. Directly upstream of the σB operon in L....... monocytogenes is the TA system mazEF, which can cleave mRNA at UACMU sites. In this study, we showed that the mazEF TA locus does not affect the level of persister formation during treatment with antibiotics in lethal doses, but exerts different effects according to the sub-inhibitory stress added. Growth...... it is not analogous to the system of S. aureus, suggesting a novel mode of action for MazEF in L. monocytogenes....

  17. Consumption Habits and Humps

    DEFF Research Database (Denmark)

    Kraft, Holger; Munk, Claus; Seifried, Frank Thomas

    2017-01-01

    We show that the optimal consumption of an individual over the life cycle can have the hump shape (inverted U-shape) observed empirically if the preferences of the individual exhibit internal habit formation. In the absence of habit formation, an impatient individual would prefer a decreasing...... consumption path over life. However, because of habit formation, a high initial consumption would lead to high required consumption in the future. To cover the future required consumption, wealth is set aside, but the necessary amount decreases with age which allows consumption to increase in the early part...... of life. At some age, the impatience outweighs the habit concerns so that consumption starts to decrease. We derive the optimal consumption strategy in closed form, deduce sufficient conditions for the presence of a consumption hump, and characterize the age at which the hump occurs. Numerical examples...

  18. Consumption Habits and Humps

    DEFF Research Database (Denmark)

    Kraft, Holger; Munk, Claus; Seifried, Frank Thomas

    We show that the optimal consumption of an individual over the life cycle can have the hump shape (inverted U-shape) observed empirically if the preferences of the individual exhibit internal habit formation. In the absence of habit formation, an impatient individual would prefer a decreasing...... consumption path over life. However, because of habit formation, a high initial consumption would lead to high required consumption in the future. To cover the future required consumption, wealth is set aside, but the necessary amount decreases with age which allows consumption to increase in the early part...... of life. At some age, the impatience outweighs the habit concerns so that consumption starts to decrease. We derive the optimal consumption strategy in closed form, deduce sufficient conditions for the presence of a consumption hump, and characterize the age at which the hump occurs. Numerical examples...

  19. A logística no comércio eletrônico b2c: um estudo nacional multicasos

    Directory of Open Access Journals (Sweden)

    Geraldo José Ferraresi de Araújo

    2011-07-01

    Full Text Available O modo de se fazer negócios está enfrentando uma revolução. O comércio eletrônico (e-commerce é um exemplo desses novos procedimentos que surgiram. Este artigo buscou estudar como as empresas varejistas brasileiras, relacionadas à venda direta de produtos aos consumidores finais, através da Internet, utilizam o comércio eletrônico (business to consummer (B2C. Foram realizadas pesquisas do tipo qualitativo-exploratória, utilizando a técnica de multicasos para verificar como as empresas realizam as atividades de processamento de pedidos, controle de estoques, localização de depósitos, embalagens, transporte e previsão da demanda. Os resultados mostram que apesar das pequenas empresas encontrarem dificuldades com a logística, elas obtiveram desempenhos razoáveis quando comparadas com as grandes empresas, sendo a Internet a oportunidade de realização de novos negócios. Igualmente, as empresas percebem que a logística é essencial para o sucesso no varejo virtual, pois possui papel significante na atividade de atender às necessidades dos clientes. Entretanto, a maior parte dos operadores logísticos precisa melhor adequar-se às necessidades do comercio eletrônico. Assim, apesar dos resultados apresentados indicarem a existência de problemas em relação às atividades logísticas no B2C, como controle de estoque e ressuprimento, as empresas pesquisadas procuraram resolver estes problemas.Abstract The way of doing business are facing a revolution. Electronic commerce (ecommerce is an example of these new procedures that have arisen. This paper aims to study how retailers in Brazil, related to selling products directly to end consumers via the Internet, using e-commerce (business to consummate (B2C. Surveys were carried out a qualitative and exploratory, using the technique ofmulticases to see how companies perform the activities of order processing, inventory control, warehouse location, packaging, transportation and demand

  20. Repeatability of tumour hypoxia imaging using [{sup 18}F]EF5 PET/CT in head and neck cancer

    Energy Technology Data Exchange (ETDEWEB)

    Silvoniemi, Antti [University of Turku, Turku PET Centre (Finland); Turku University Hospital, Department of Otorhinolaryngology - Head and Neck Surgery (Finland); Suilamo, Sami [Turku University Hospital, Department of Oncology and Radiotherapy (Finland); Turku University Hospital, Department of Medical Physics (Finland); Laitinen, Timo; Forsback, Sarita; Solin, Olof [University of Turku, Turku PET Centre (Finland); Loeyttyniemi, Eliisa [University of Turku, Department of Biostatistics, Turku (Finland); Vaittinen, Samuli [Turku University Hospital, Department of Pathology (Finland); Saunavaara, Virva [University of Turku, Turku PET Centre (Finland); Turku University Hospital, Department of Medical Physics (Finland); Groenroos, Tove J.; Minn, Heikki [University of Turku, Turku PET Centre (Finland); Turku University Hospital, Department of Oncology and Radiotherapy (Finland)

    2018-02-15

    Hypoxia contributes to radiotherapy resistance and more aggressive behaviour of several types of cancer. This study was designed to evaluate the repeatability of intratumour uptake of the hypoxia tracer [{sup 18}F]EF5 in paired PET/CT scans. Ten patients with newly diagnosed head and neck cancer (HNC) received three static PET/CT scans before chemoradiotherapy: two with [{sup 18}F]EF5 a median of 7 days apart and one with [{sup 18}F]FDG. Metabolically active primary tumour volumes were defined in [{sup 18}F]FDG images and transferred to co-registered [{sup 18}F]EF5 images for repeatability analysis. A tumour-to-muscle uptake ratio (TMR) of 1.5 at 3 h from injection of [{sup 18}F]EF5 was used as a threshold representing hypoxic tissue. In 10 paired [{sup 18}F]EF5 PET/CT image sets, SUVmean, SUVmax, and TMR showed a good correlation with the intraclass correlation coefficients of 0.81, 0.85, and 0.87, respectively. The relative coefficients of repeatability for these parameters were 15%, 17%, and 10%, respectively. Fractional hypoxic volumes of the tumours in the repeated scans had a high correlation using the Spearman rank correlation test (r = 0.94). In a voxel-by-voxel TMR analysis between the repeated scans, the mean of Pearson correlation coefficients of individual patients was 0.65. The mean (± SD) difference of TMR in the pooled data set was 0.03 ± 0.20. Pretreatment [{sup 18}F]EF5 PET/CT within one week shows high repeatability and is feasible for the guiding of hypoxia-targeted treatment interventions in HNC. (orig.)

  1. Predictive potential of macrophage migration inhibitory factor (MIF) in patients with heart failure with preserved ejection fraction (HFpEF).

    Science.gov (United States)

    Luedike, Peter; Alatzides, Georgios; Papathanasiou, Maria; Heisler, Martin; Pohl, Julia; Lehmann, Nils; Rassaf, Tienush

    2018-05-04

    Prognostication in heart failure with preserved ejection fraction (HFpEF) is challenging and novel biomarkers are urgently needed. Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that plays a crucial role in cardiovascular and various inflammatory diseases. Whether MIF is involved in HFpEF is unknown. Sixty-two patients with HFpEF were enrolled and followed up for 180 days. MIF plasma levels as well as natriuretic peptide (NP) levels were assessed. High MIF levels significantly predicted the combined end-point of all-cause death or hospitalization at 180 days in the univariate analysis (HR 2.41, 95% CI 1.12-5.19, p = 0.025) and after adjustment for relevant covariates in a Cox proportional hazard regression model (HR 2.35, 95% CI 1.05-5.27, p = 0.0374). Furthermore, MIF levels above the median were associated with higher pulmonary artery systolic pressure (PASP) as assessed by echocardiography (PASP 31 mmHg vs 48 mmHg in the low- and high-MIF group, respectively, p = 0.017). NPs significantly correlated with MIF in HFpEF patients (BNP p = 0.011; r = 0.32; NT-proBNP p = 0.027; r = 0.28). MIF was associated with clinical outcomes and might be involved in the pathophysiology of pulmonary hypertension in patients with HFpEF. These first data on MIF in HFpEF should stimulate further research to elucidate the role of this cytokine in heart failure. Trial registration NCT03232671.

  2. Hb taradale [beta82(EF6)Lys-->Arg]: a novel mutation at a 2,3-diphosphoglycerate binding site.

    Science.gov (United States)

    Brennan, Stephen O; Sheen, Campbell; Chan, Tim; George, Peter M

    2005-01-01

    Hb Taradale [beta82(EF6)Lys-->Arg] was initially detected as a split Hb A0 peak on Hb A1c, monitoring. Red cell parameters, hemoglobin (Hb) electrophoresis and stability tests were normal. Mass spectrometry (ms) clearly identified a variant beta chain with a mass increase of 28 Da and peptide mapping located the mutation site to peptide betaT-9. DNA sequencing confirmed the presence of a novel beta82(EF6)Lys-->Arg mutation. This conservative substitution at a 2,3-diphosphoglycerate (2,3-DPG) binding site did not, however, appear to affect the P50 for oxygen binding.

  3. Os impactos da Nota Fiscal eletrônica nas microempresas e empresas de pequeno porte sediadas em Umuarama/PR

    Directory of Open Access Journals (Sweden)

    Luiz Carlos da Silva

    2014-03-01

    Full Text Available A globalização propiciou mudanças de hábitos, de consumo, de produção de bens, de serviços e de conhecimento. A contabilidade, ferramenta imprescindível para a tomada de decisão empresarial e governamental, é parte inerente nesse contexto em virtude, principalmente, do seu papel social. Nesse cenário, o Governo Federal instituiu, por meio do Decreto nº 6.022, de 22 de janeiro de 2007, o Sistema Público de Escrituração Digital (SPED que, dentre seus subprojetos, propõe a Nota Fiscal Eletrônica (NF-e, processo eletrônico de emissão de notas fiscais que utiliza a Tecnologia de Informação para reduzir as despesas com papel e armazenagem de documentos, e contribui para a diminuição das obrigações acessórias. A presente pesquisa objetivou analisar os impactos causados nas Microempresas (MEs e Empresas de Pequeno Porte (EPPs sediadas em Umuarama/PR, quanto à implantação da NF-e e a percepção dos gestores sobre os objetivos e benefícios preconizados pelo Governo Federal. Esta pesquisa, quanto à sua natureza, é classificada como aplicada, descritiva, indutiva, com caráter exploratório e de abordagem quanti-qualitativa. O instrumento de pesquisa e levantamento de dados adotado foi um questionário semiestruturado com perguntas abertas e fechadas, aplicado em setembro de 2011 com uma amostra de pesquisa composta por trinta empresas do município escolhido. Os resultados apontam que os principais impactos sofridos com a implantação da NF-e foram: mudança de processos internos, necessidade de investimentos em tecnologia e aumento nos custos totais, bem como a divergência parcial do preconizado pelo Governo Federal no que concerne à percepção do empresariado relativamente aos objetivos e benefícios da ferramenta.  

  4. Logística reversa no comércio eletrônico: um estudo de caso Reverse logistics in the e-commerce: a case study

    Directory of Open Access Journals (Sweden)

    Ana Carolina de Araujo

    2013-06-01

    Full Text Available A logística reversa ganha importância cada vez maior tanto no meio acadêmico quanto entre os profissionais de supply chain, não só por ser um dos instrumentos para a prática da sustentabilidade, como também por seu papel estratégico em muitos segmentos econômicos. Diversos trabalhos mostram como uma logística reversa eficiente pode diminuir custos e propiciar vantagem competitiva às empresas. Da mesma forma, o comércio eletrônico vem apresentando crescimento expressivo em muitos países e, por sua própria natureza apresenta taxas de retorno muito maiores que o comércio tradicional. Contudo, apesar da importância da operação de logística reversa para o comércio eletrônico, não se encontram trabalhos que explorem esse tema na literatura. O objetivo deste trabalho é descrever o processo de logística reversa do maior varejista virtual do mercado brasileiro, mostrando sua evolução, analisando seu desempenho e identificando suas principais deficiências. Conclui-se com a apresentação de sugestões para melhorias e implantação de um sistema mais eficiente.Reverse logistics has gained increasing importance among researchers and supply chain professionals not only for being one of the tools for sustainability, but also for its strategic role in many economic sectors. Several studies have shown how an efficient reverse logistics can reduce costs and provide competitive advantage to companies. Likewise, there has been a significant growth of electronic commerce in many countries, which has much higher return rates than those of traditional trade. Nevertheless, despite the importance of reverse logistics in electronic commerce, there are no studies addressing this topic in the literature. The aim of this paper is to describe the reverse logistics process of the largest online retailer in the Brazilian market by showing its development, analyzing its performance, and identifying its major deficiencies. Some suggestions for the

  5. Implantes eletrônicos para restabelecimento da visão em cegos Electronic implants to restore vision in blind patients

    Directory of Open Access Journals (Sweden)

    Eduardo Büchele Rodrigues

    2004-04-01

    Full Text Available O sucesso recente no desenvolvimento de uma prótese eletrônica coclear para surdos estimulou vários grupos de cientistas ao desenvolvimento de próteses visuais. A maioria dos protótipos de próteses visuais está baseada em estimulação elétrica neuronal em diferentes localizações do sistema visual até o sistema nervoso central. Atualmente os esforços estão concentrados em três localizações de implantes visuais: retina, nervo óptico e córtex. Implantes de retina e do nervo óptico têm o potencial de restabelecer a visão em pacientes com degenerações retinianas progressivas por meio de estimulação elétrica de neurônios do sistema visual. Próteses corticais podem beneficiar um número maior de pacientes cegos devido à sua localização mais posterior no sistema visual. Apesar dos grandes avanços, torna-se ainda necessária a elucidação de questões importantes na avaliação do funcionamento, em longo prazo, dos vários implantes eletrônicos para cegos, em estudo. Neste artigo analisamos os motivos que justificam o início dos experimentos nas três posições mencionadas e os desafios advindos de tal decisão.Recent success in the development of a prosthesis for the deaf patient encouraged several groups of scientists to develop and investigate a visual prosthesis. Visual prosthesis are based on neuronal electrical stimulation at different locations along the visual pathways, and three localizations of visual prosthesis are being researched, retina, optic nerve, and cortex. Retina and optic nerve implants may restore vision in patients with progressive retinal degenerations by means of electrical stimulation of visual pathway neurons. Cortex prosthesis may restore vision in a greater number of blind patients due to the more posterior location in the visual pathway. While major advances have been made in the field of visual prosthesis for the blind, a number of key questions remain to render a visual prosthesis

  6. Ouça sua banda preferida sem precisar ir atrás daquele Torrent:uma análise da publicidade das empresas eletrônicas de música

    Directory of Open Access Journals (Sweden)

    Leonardo De Marchi

    2014-12-01

    Full Text Available Neste artigo, realiza-se uma análise do discurso de peças publicitárias de empresas eletrônicas estrangeiras de música que começam a operar no Brasil. O objetivo do trabalho é categorizar as estratégias retóricas utilizadas pelas empresas eletrônicas e compreender como tais produtos midiáticos ajudam na construção de um discurso sobre esses novos agentes da indústria fonográfica. Conclui-se que se têm uma concepção dos consumidores brasileiros como reticentes a pagar para consumir música nas redes digitais, mas interessados em se socializar através de seu gosto musical.

  7. Governo Eletrônico no Espírito Santo e a Possibilidade de Ampliação da Participação Política

    OpenAIRE

    SANTOS, L. G. M.

    2013-01-01

    O presente trabalho tem por objetivo estudar o cenário do governo eletrônico no Governo do Estado do Espírito Santo, identificando e analisando os estágios de maturidade em que se encontra o portal www.es.gov.br, conforme os conceitos ONU (2003, 2008) acerca do uso das tecnologias de informação e comunicação, e averiguar a possibilidade ampliação da participação política através do sítio eletrônico do PPA Online que recebe as demandas da população a serem incorporadas no plano plurianual esta...

  8. Efeitos do Comércio Eletrônico: a percepção dos empresários associados à Câmara de Dirigentes Lojistas (CDL) de São Miguel do Oeste-SC

    OpenAIRE

    Dayani Gwozdz; Josiane Romancini; Karina Fries; Lucas Miguel Gnigler; Sodriane Cezar

    2014-01-01

    Diante do cenário econômico atual, os empresários buscam agilidade, facilidade e precisão nos processos de venda e gestão de estoques. O comércio eletrônico propõe ao empresário uma forma diferenciada para disponibilizar seu produto ou serviço em um ambiente virtual ou, ainda, fazer uso dessa ferramenta em transações com indústria e fornecedores. O principal objetivo desta pesquisa foi verificar as consequências do comércio eletrônico para os comerciantes do município de São Miguel do Oeste-S...

  9. Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues.

    Science.gov (United States)

    Sato, Aya; Suematsu, Takuma; Aihara, Koh-Ki; Kita, Kiyoshi; Suzuki, Tsutomu; Watanabe, Kimitsuna; Ohtsuki, Takashi; Watanabe, Yoh-Ichi

    2017-03-07

    Translation elongation factor Tu (EF-Tu) delivers aminoacyl-tRNA (aa-tRNA) to ribosomes in protein synthesis. EF-Tu generally recognizes aminoacyl moieties and acceptor- and T-stems of aa-tRNAs. However, nematode mitochondrial (mt) tRNAs frequently lack all or part of the T-arm that is recognized by canonical EF-Tu. We previously reported that two distinct EF-Tu species, EF-Tu1 and EF-Tu2, respectively, recognize mt tRNAs lacking T-arms and D-arms in the mitochondria of the chromadorean nematode Caenorhabditis elegans C. elegans EF-Tu2 specifically recognizes the seryl moiety of serylated D-armless tRNAs. Mitochondria of the enoplean nematode Trichinella possess three structural types of tRNAs: T-armless tRNAs, D-armless tRNAs, and cloverleaf tRNAs with a short T-arm. Trichinella mt EF-Tu1 binds to all three types and EF-Tu2 binds only to D-armless Ser-tRNAs, showing an evolutionary intermediate state from canonical EF-Tu to chromadorean nematode (e.g. C. elegans ) EF-Tu species. We report here that two EF-Tu species also participate in Drosophila melanogaster mitochondria. Both D. melanogaster EF-Tu1 and EF-Tu2 bound to cloverleaf and D-armless tRNAs. D. melanogaster EF-Tu1 has the ability to recognize T-armless tRNAs that do not evidently exist in D. melanogaster mitochondria, but do exist in related arthropod species. In addition, D. melanogaster EF-Tu2 preferentially bound to aa-tRNAs carrying small amino acids, but not to aa-tRNAs carrying bulky amino acids. These results suggest that the Drosophila mt translation system could be another intermediate state between the canonical and nematode mitochondria-type translation systems. © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society.

  10. Energy Consumption Database

    Science.gov (United States)

    Consumption Database The California Energy Commission has created this on-line database for informal reporting ) classifications. The database also provides easy downloading of energy consumption data into Microsoft Excel (XLSX

  11. Morfologia da dentina tratada com substâncias dessensibilizantes: avaliação através da microscopia eletrônica de varredura

    Directory of Open Access Journals (Sweden)

    ODA Margareth

    1999-01-01

    Full Text Available As constantes recidivas dos tratamentos preconizados para a hipersensibilidade dentinária são causa de estudos a respeito desta patologia. Neste trabalho, in vitro, nos propomos a avaliar, por meio de microscopia eletrônica de varredura, a possível formação de película impermeabilizadora quando da aplicação de substâncias dessensibilizantes, acompanhada de condicionamento ácido da dentina. Discos de dentina, foram utilizados onde foi preparado um esfregaço padronizado, realizado condicionamento ácido e aplicação das substâncias testadas, à base de glutaraldeído, oxalato e fluoretos. Para compor o grupo controle um corpo-de-prova foi mantido sem tratamento e outro apenas condicionado com ácido. Dos resultados morfológicos obtidos pode-se concluir que o condicionamento ácido prévio ao tratamento com substâncias à base de glutaraldeído e de oxalato não é capaz de formar uma película uniforme, impermeabilizante sobre a dentina. O uso de fluoretos forma uma camada sobre a dentina facilmente removível, não produzindo porém efeitos a longo prazo nestes tipos de tratamento.

  12. Ensino de Física para surdos: um experimento mecânico e um eletrônico para o ensino de ondas sonoras

    Directory of Open Access Journals (Sweden)

    Deise Benn Pereira Vivas

    2017-05-01

    Full Text Available Este trabalho se insere na temática sobre o papel das Tecnologias Assistivas na compreensão conceitual e na promoção da inserção social de estudantes surdos. São apresentadas duas versões (mecânica e eletrônica de um experimento didático desenvolvido pelos autores para o ensino de ondas sonoras, voltado primordialmente para estudantes surdos do Ensino Médio. Inicialmente, é feito um breve histórico da educação para surdos desde a antiguidade, seguida de uma discussão teórica sobre a física da voz. Na sequência, são apresentadas as duas versões do experimento com seus detalhes técnicos e o processo de elaboração dos mesmos, bem como, uma discussão teórica acerca de uma perspectiva para uso didático destes experimentos, orientado por uma visão vigotskiana. Ao final, são feitas considerações acerca do potencial uso didático do experimento visando a inclusão dos alunos surdos em classes regulares, visto que se trata de experimentos ainda não avaliados em situações reais de sala de aula.

  13. O pregão eletrônico e as contratações de serviços contínuos

    Directory of Open Access Journals (Sweden)

    Marcelo de Freitas

    2013-10-01

    Full Text Available A economia gerada pela utilização do pregão eletrônico nas licitações promovidas pelos órgãos públicos tem sido amplamente destacada. No entanto, ainda existem poucas informações sistematizadas sobre os impactos deste instrumento na execução e na gestão dos contratos que dele se originam. Este artigo se propõe a analisar e comentar a forma pela qual é alcançada a redução de custos nas contratações de serviços contínuos pela Administração Pública e os possíveis efeitos desta redução tanto para as organizações contratantes como para os prestadores de serviços terceirizados. Quanto à metodologia, o estudo, de natureza exploratória, constituiu-se de pesquisa bibliográfica, compreendendo a produção científica e a produção normativa sobre o tema, além de análise documental.

  14. Resolução lamelar num novo microscópio eletrônico de varredura Lamellar resolution in a new scanning electron microscope

    Directory of Open Access Journals (Sweden)

    Hans-Jürgen Kestenbach

    1997-03-01

    Full Text Available RESUMO: Trabalhando com elétrons de baixa energia (na faixa de 1keV, o novo microscópio eletrônico de varredura dispensa a etapa de metalização e permite a observação direta da estrutura lamelar de polímeros semicristalinos, sem a necessidade de preparação de amostras. São apresentados exemplos da morfologia lamelar do PVDF em função das condições de processamento e da temperatura de cristalização, em filmes contendo as fases a, b e g. Um outro exemplo revela o crescimento inicial da camada transcristalina que se formou ao longo de uma fibra de polietileno de ultra-alto peso molecular embutida em matriz de polietileno de alta densidade.ABSTRACT: Working with low energy electrons (in the range of 1keV, the new scanning electron microscope permits the lamellar (supermolecular structure of semicrystalline polymers to be observed directly without the need of specimen coating or of any other sample preparation technique. Microscope performance is demonstrated by several examples of high resolution micrographs which show spherulitic, lamellar and fibrilar morphologies developed by the a, b and g phases of PVDF as a function of processing conditions and crystallization temperature. Another example reveals the early stages of transcrystalline layer formation in HDPE reinforced by UHMWPE fibers.

  15. Aplicação do seis sigma em recursos humanos: um estudo de caso em uma empresa eletro-eletrônica

    Directory of Open Access Journals (Sweden)

    Renato Luis de Boer

    2010-06-01

    Full Text Available Este trabalho apresenta um caso de aplicação da metodologia Seis Sigma na área de recursos humanos de uma empresa de manufatura de componentes eletrônicos. Objetivou-se analisar como a metodologia Seis Sigma foi aplicada e que adaptações se fizeram necessárias para um resultado eficaz. O projeto de Seis Sigma em análise tinha por objetivo a redução da rotatividade de pessoal, ou seja, reduzir o turn over de afastamento. O estudo de caso foi o método de pesquisa utilizado. A estrutura DMAIC (define, measure, analyse, improve and control foi utilizada no desenvolvimento deste projeto de Seis Sigma. As ferramentas foram apropriadas para dados qualitativos. As principais dificuldades relacionaram-se a incoerência entre dados provenientes de diversas fontes, receio de atribuição de culpa pela alta rotatividade de certas funções da empresa e apreensão quanto ao uso de ferramentas típicas do Seis Sigma, fortemente baseadas em estatística e análise quantitativa. O projeto Seis Sigma atingiu as metas estabelecidas além de gerar envolvimento de diversas áreas da empresa com a gestão de pessoas.

  16. Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

    Directory of Open Access Journals (Sweden)

    Ševčíková Zuzana

    2016-09-01

    Full Text Available Introduction: The present study aimed to investigate the effect of Enterococcus faecium EF55 on chickens, as well as its influence on proliferative activity of epithelial intestinal cells after infection with Salmonella enterica serovar Enteritidis phage type 4 (SE PT4. Moreover, the length and area of duodenal and jejunal villi of the birds were examined.

  17. Efímero Festín, el video danza antártico

    OpenAIRE

    Pombo, Ernesto; Costa Chimene

    2013-01-01

    Efímero Festín es un video danza pensado específicamente para ser realizado en la Antártida argentina. Pretende a través del arte y de una forma poética, colaborar con la concientización del cuidado del medio ambiente.

  18. Neurite outgrowth mediated by translation elongation factor eEF1A1: a target for antiplatelet agent cilostazol.

    Directory of Open Access Journals (Sweden)

    Kenji Hashimoto

    Full Text Available Cilostazol, a type-3 phosphodiesterase (PDE3 inhibitor, has become widely used as an antiplatelet drug worldwide. A recent second Cilostazol Stroke Prevention Study demonstrated that cilostazol is superior to aspirin for prevention of stroke after an ischemic stroke. However, its precise mechanisms of action remain to be determined. Here, we report that cilostazol, but not the PDE3 inhibitors cilostamide and milrinone, significantly potentiated nerve growth factor (NGF-induced neurite outgrowth in PC12 cells. Furthermore, specific inhibitors for the endoplasmic reticulum protein inositol 1,4,5-triphosphate (IP(3 receptors and several common signaling pathways (PLC-γ, PI3K, Akt, p38 MAPK, and c-Jun N-terminal kinase (JNK, and the Ras/Raf/ERK/MAPK significantly blocked the potentiation of NGF-induced neurite outgrowth by cilostazol. Using a proteomics analysis, we identified that levels of eukaryotic translation elongation factor eEF1A1 protein were significantly increased by treatment with cilostazol, but not cilostamide, in PC12 cells. Moreover, the potentiating effects of cilostazol on NGF-induced neurite outgrowth were significantly antagonized by treatment with eEF1A1 RNAi, but not the negative control of eEF1A1. These findings suggest that eEF1A1 and several common cellular signaling pathways might play a role in the mechanism of cilostazol-induced neurite outgrowth. Therefore, agents that can increase the eEF1A1 protein may have therapeutic relevance in diverse conditions with altered neurite outgrowth.

  19. A publicação de dados governamentais abertos: proposta de revisão da classe sobre Previdência Social do Vocabulário Controlado do Governo Eletrônico

    Directory of Open Access Journals (Sweden)

    Cláudio José Silva Ribeiro

    Full Text Available Este trabalho analisa a classe sobre Previdência Social do Vocabulário Controlado do Governo Eletrônico, investigando a publicação de dados governamentais abertos, realizada pela Empresa de Tecnologia e Informações da Previdência Social. Trata-se de um estudo exploratório, com objetivo de propor a revisão da classe sobre Previdência Social no domínio do Governo Eletrônico brasileiro, a fim de ampliar e corrigir a representação da área. O trabalho utiliza como metodologia: (1 pesquisa bibliográfica, para fundamentação teórica; (2 seleção da classe de assunto sobre Previdência Social do Vocabulário Controlado do Governo Eletrônico, como recorte; (3 análise dos pontos relacionados à categorização, linguagem escolhida (natural ou artificial, cobertura dos termos pertencentes ao escopo da classe de assunto estudado e relações apresentadas entre os termos; (4 propostas para revisão da classe estudada. O estudo aponta, nos resultados, falhas estruturais e inconsistências conceituais localizados no vocabulário. Discute a necessidade de se utilizarem vocabulários consistentes para representação dos dados publicados, a fim de torná-los legíveis por máquina e compartilháveis entre aplicações de software. Afirma que se deve investir em vocabulários já existentes para evitar esforços com a criação de outros instrumentos de representação. Julga necessário que outros órgãos públicos, além da Empresa de Tecnologia e Informações da Previdência Social, interessem-se em analisar e propor alterações e atualizações nas classes cuja temática contemple os dados por eles geridos. Conclui que a utilização do Vocabulário Controlado do Governo Eletrônico pode ir além de um mapa para navegação em páginas eletrônicas do setor público brasileiro, constituindo instrumento dinâmico de organização do conhecimento, podendo ser utilizado para diversos fins, de acordo com os interesses de cada usuário.

  20. Versão eletrônica de questionário e o controle de erros de resposta Electronic version of a questionnaire and control of answer errors

    Directory of Open Access Journals (Sweden)

    Sadao Omote

    2005-12-01

    Full Text Available O artigo relata a análise de erros cometidos em questionário impresso e a aplicabilidade de uma versão eletrônica do mesmo questionário para o controle desses erros. Sessenta estudantes de pós-graduação em Educação responderam à versão eletrônica programada em Visual Basice outros 52 responderam à versão impressa. No questionário impresso, foram cometidos 95 erros, dos quais 28 não invalidam as respostas. Os demais erros podem levar à exclusão dos participantes que os cometeram, se todos os itens forem analisados rigorosamente de acordo com as instruções. Os erros cometidos na versão impressa podem ser controlados na versão eletrônica mediante adequada programação. Nenhuma dificuldade para responder a versão eletrônica foi identificada. As vantagens apontadas pelos respondentes e a possibilidade de controle total dos erros de resposta, aliadas à eliminação de erro de tabulação mediante a inserção automática das respostas em banco de dados, recomendam o uso de versão eletrônica de questionário.The article reports the analysis of errors made in a printed questionnaire and the possibility of using an electronic version of the same questionnaire aiming to control those errors. Sixty Education graduate students answered an electronic version, programmed in Visual Basic, and another 52 answered a printed version. In the printed questionnaire, 95 errors were made of which 28 errors may be ignored. The other errors may cause the exclusion of respondents that made them, if all items were analyzed strictly in agreement with the instructions. The errors made in the printed version may be controlled in an electronic version by means of appropriate programming. No difficulty in answering the electronic version was reported. The advantages pointed out by the respondents and the possibility of total control of answer errors, allied with the elimination of tabulation errors by means of automatic database insertion of the

  1. A Supervisory Control Algorithm of Hybrid Electric Vehicle Based on Adaptive Equivalent Consumption Minimization Strategy with Fuzzy PI

    Directory of Open Access Journals (Sweden)

    Fengqi Zhang

    2016-11-01

    Full Text Available This paper presents a new energy management system based on equivalent consumption minimization strategy (ECMS for hybrid electric vehicles. The aim is to enhance fuel economy and impose state of charge (SoC charge-sustainability. First, the relationship between the equivalent factor (EF of ECMS and the co-state of pontryagin’s minimum principle (PMP is derived. Second, a new method of implementing the adaptation law using fuzzy proportional plus integral (PI controller is developed to adjust EF for ECMS in real-time. This adaptation law is more robust than one with constant EF due to the variation of EF as well as driving cycle. Finally, simulations for two driving cycles using ECMS are conducted as opposed to the commonly used rule-based (RB control strategy, indicating that the proposed adaptation law can provide a promising blend in terms of fuel economy and charge-sustainability. The results confirm that ECMS with Fuzzy PI adaptation law is more robust than ECMS with constant EF as well as PI adaptation law and it achieves significant improvements compared with RB in terms of fuel economy, which is enhanced by 4.44% and 14.7% for china city bus cycle and economic commission of Europe (ECE cycle, respectively.

  2. Remodeling of the AB site of rat parvalbumin and oncomodulin into a canonical EF-hand

    DEFF Research Database (Denmark)

    Cox, J A; Durussel, I; Scott, D J

    1999-01-01

    -residue canonical loop. To create an optical conformational probe we also expressed the homologs with a F102W replacement. Unexpectedly, in none of the proteins did the mutation reactivate the AB site. The AB-remodeled parvalbumins bind two Ca2+ ions with strong positive cooperativity (nH = 2......Parvalbumin (PV) and the homologous protein oncomodulin (OM) contain three EF-hand motifs, but the first site (AB) cannot bind Ca2+. Here we aimed to recreate the putative ancestral proteins [D19-28E]PV and [D19-28E]OM by replacing the 10-residue-long nonfunctional loop in the AB site by a 12...... conformations. The AB-remodeled oncomodulins also bind two Ca2+ with [Ca2+]0.5 = 43 microM and nH = 1.45. Mg2+ does not affect Ca2+ binding. Again the Ca2+ forms display two-thirds of the alpha-helical content in the wild-type, while their core is still strongly hydrophobic as monitored by Trp and Tyr...

  3. Application of BET_EF at Mount Etna: a retrospective analysis (years 2001-2005

    Directory of Open Access Journals (Sweden)

    Jacopo Selva

    2011-12-01

    Full Text Available Advances in volcano monitoring and forecasting need a multidisciplinary collaborative framework. In light of this, a Bayesian Event Tree (BET approach was performed by the application of the BET for Eruption Forecasting (BET_EF code to analyze the space-time distribution of the volcanic activity of Mount Etna from 2001-2005. First, a reliable monitoring dataset was set up after some sessions to elicit geophysical, volcanological and geochemical ‘precursor’ parameters. A constant unrest probability of 100%, with a magma involvement usually greater than 95%, was computed throughout the time period analyzed. Eruption probabilities higher than 90% were estimated a few days before the onsets of the 2001 and 2002-2003 flank eruptions. Values slightly higher than 75% were observed during the lava fountaining period in June-July 2001. However, the probabilities flattened to around 30% for the 2004-2005 flank eruption. With suitable data, a good depiction of the actual location of the eruptive scenario for the 2001 and 2002-2003 events was provided. Conversely, the size of the eruptions was not indicated.

  4. A safe potential juice clarifying pectinase from Trichoderma viride EF-8 utilizing Egyptian onion skins

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    Abdel-Mohsen S. Ismail

    2016-06-01

    Full Text Available The production of a notable, safe and highly active pectinase by the local fungal strain Trichoderma viride EF-8 utilizing the abundant pigmented Egyptian onion (Allium cepa L. skins (6.5%, w/v was achieved in 4 days submerged fermentation (SMF cultures, at temperature and pH of 30 °C and 4.0, respectively. The indigenously produced pectinase was partially purified by 50% batch ethanol precipitation and its general properties were studied following the standard procedures. The lyophilized enzyme preparation was free of any ochra or aflatoxins. The optimum conditions for the partially purified enzyme form were 2 mg/mL and 1% (w/v enzyme protein and substrate (citrus pectin concentrations, reaction pH and temperature of 7.0 and 40 °C, respectively. The results presented the low cost onion skins waste as the major substrate for the fungal pectinase production and its subsequent use in perfect fruit (apple, lemon and orange juices clarification with remarkable stability during and after this process, which certainly enhance fruit juices processing in the tropics.

  5. Vurdering af effekten af en vindmøllepark ved Overgaard på forekomsten af fugle i EF-fuglebeskyttelsesområde nr. 15

    DEFF Research Database (Denmark)

    Clausen, P.; Larsen, J. K.

    forekomsten af en række arter der enten yngler eller raster inden for EF-fuglebeskyttelsesområdet, og er omfattet af EF-fuglebeskyttelsesdirektivets bilag I. Det drejer sig om arterne skarv Phalacrocorax carbo sinensis, blå kærhøg Circus cyaneus, hjejle Pluvialis apricaria, brushane Philomachus pugnax, klyde...

  6. The eukaryotic translation elongation factor eEF1A2 induces neoplastic properties and mediates tumorigenic effects of ZNF217 in precursor cells of human ovarian carcinomas

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Yu; Wong, Nicholas; Guan, Yinghui; Salamanca, Clara M.; Cheng, Jung Chien; Lee, Jonathan M.; Gray, Joe W.; Auersperg, Nelly

    2008-04-25

    Ovarian epithelial carcinomas (OEC) frequently exhibit amplifications at the 20q13 locus which is the site of several oncogenes, including the eukaryotic elongation factor EEF1A2 and the transcription factor ZNF217. We reported previously that overexpressed ZNF217 induces neoplastic characteristics in precursor cells of OEC. Unexpectedly, ZNF217, which is a transcriptional repressor, enhanced expression of eEF1A2. In this study, array comparative genomic hybridization, single nucleotide polymorphism and Affymetrix analysis of ZNF217-overexpressing cell lines confirmed consistently increased expression of eEF1A2 but not of other oncogenes, and revealed early changes in EEF1A2 gene copy numbers and increased expression at crisis during immortalization. We defined the influence of eEF1A2 overexpression on immortalized ovarian surface epithelial cells, and investigated interrelationships between effects of ZNF217 and eEF1A2 on cellular phenotypes. Lentivirally induced eEF1A2 overexpression caused delayed crisis, apoptosis resistance and increases in serum-independence, saturation densities, and anchorage independence. siRNA to eEF1A2 reversed apoptosis resistance and reduced anchorage independence in eEF1A2-overexpressing lines. Remarkably, siRNA to eEF1A2 was equally efficient in inhibiting both anchorage independence and resistance to apoptosis conferred by ZNF217 overexpression. Our data define neoplastic properties that are caused by eEF1A2 in nontumorigenic ovarian cancer precursor cells, and suggest that eEF1A2 plays a role in mediating ZNF217-induced neoplastic progression.

  7. [birthweight And Caffeine Consumption].

    OpenAIRE

    Bicalho, Gladys Gripp; Barros Filho, Antônio de Azevedo

    2015-01-01

    To assess the association between maternal caffeine consumption during pregnancy and low birth weight, prematurity and intrauterine growth retardation. A case-control was carried out and 354 newborns of single labor with birthweight 3,000 g (controls) were analyzed. Caffeine consumption was calculated based on daily consumption of coffee, soft drinks and tea. Results were adjusted using multiple logistic regression for the following confounders: mother's age, schooling, income, marital status...

  8. Alcohol Consumption in Students

    OpenAIRE

    Tran, Cathy

    2010-01-01

    Drinking behaviour among university students is a serious public health concern. Reasons for drinking are complex and many factors contribute to this behaviour. Previous research has established links between personality factors and alcohol consumption and also between metacognitions and alcohol consumption. Few studies have looked into how personality traits and metacognitions interact. This study investigated the relationships between personality, metacognitions and alcohol consumption in a...

  9. A Ca2+-calmodulin-eEF2K-eEF2 signalling cascade, but not AMPK, contributes to the suppression of skeletal muscle protein synthesis during contractions

    DEFF Research Database (Denmark)

    Rose, Adam John; Alsted, Thomas Junker; Jensen, Thomas Elbenhardt

    2009-01-01

    Skeletal muscle protein synthesis rate decreases during contractions but the underlying regulatory mechanisms are poorly understood. It was hypothesised that there would be a coordinated regulation of eukaryotic elongation factor 2 (eEF2) and eukaryotic initiation factor 4E-binding protein 1 (4EBP1......) phosphorylation by signalling cascades downstream of rises in intracellular [Ca(2+)] and decreased energy charge via AMP activated protein kinase (AMPK) in contracting skeletal muscle. When fast-twitch skeletal muscles were contracted ex vivo using different protocols, the suppression of protein synthesis...... correlated more closely with changes in eEF2 rather than 4EBP1 phosphorylation. Using a combination of Ca(2+) release agents and ATPase inhibitors it was shown that the 60-70% suppression of fast-twitch skeletal muscle protein synthesis during contraction was equally distributed between Ca(2+) and energy...

  10. Precificação estratégica no comércio eletrônico – B2c (business to consumer

    Directory of Open Access Journals (Sweden)

    Joisse Antonio Lorandi

    2015-06-01

    Full Text Available Na formação de uma precificação estratégica, a ênfase deve situar-se na transmissão de valor. O preço estabelecido deve representar o valor agregado em relação a satisfação que o produto e todo processo de atendimento propiciam ao cliente. A transmissão de valor no comércio eletrônico deve priorizar a identificação do que é considerado um diferencial, entre o varejo virtual e em relação ao seu principal concorrente, o varejo tradicional. É neste âmbito que a pesquisa se desenvolve, tendo como objetivos identificar fatores que agregam valor no comércio virtual e parâmetros que devem balizar a precificação estratégica neste ambiente, como os componentes de custos e a concorrência. A metodologia do estudo quanto aos procedimentos técnicos é de pesquisa bibliográfica, a qual foi elaborada por meio deum referencial teórico que está formando-se em torno do assunto. Por se tratar de um modelo recente, em formação, caracteriza-se a pesquisa quanto a seus objetivos, como de caráter exploratório, principalmente no âmbito da formação do preço, já que há uma lacuna entre a forma de atuar no varejo virtual em relação ao tradicional. O estudo explora esta lacuna por meio da identificação de elementos que formam o valor do produto comercializado, via rede eletrônica de dados, concluindo que a precificação estratégica para uma lucratividade efetiva deve representar o valor do produto, uma vez que o que se vende é o valor que o produto agrega ao cliente e não componentes de custos mais uma margem. In the formation of strategic price, the principle should be the transmission of value,the final price must be represent the aggregate value in relation to what, the productand all the services involved, bring to the buyer. In the electronic market, the valueinput should prioritize the identification of what it is consider a differential betweenthe virtual and the traditional market. That is how the study progresses

  11. Uma visão arquivística do sistema de processo judicial eletrônico do Conselho Nacional de Justiça

    Directory of Open Access Journals (Sweden)

    Leonardo Neves Moreira

    2015-12-01

    Full Text Available O sistema Processo Judicial Eletrônico (PJe é uma inovação que vem ganhando espaço em todos os ramos do Poder Judiciário. Esse software foi desenvolvido pelo Conselho Nacional de Justiça (CNJ e tem como premissa básica a substituição de processos judiciais em suporte papel por ações visualizadas e tramitadas exclusivamente em meio digital. A substituição do papel visa principalmente racionalizar os procedimentos jurisdicionais e conceder maior celeridade ao trâmite das ações. O PJe traz uma mudança de paradigma que impacta profundamente a atuação de servidores, advogados e juízes. No que tange à garantia de confiabilidade e autenticidade dos autos de processos judiciais, nota-se que a linha de desenvolvimento do PJe prioriza particularidades tecnológicas e jurídicas deixando em segundo plano aspectos relacionados ao gerenciamento arquivístico dos processos armazenados no sistema. Notadamente, há lacunas no que se refere à compreensão do PJe enquanto fenômeno informacional. Nesse contexto, esta pesquisa visa situar o PJe no âmbito dos estudos sobre informação arquivística, ressaltando a importância do Modelo de Requisitos para Sistema Informatizados de Gestão de Processos e Documentos do Judiciário brasileiro (MoReq-Jus como elemento basilar para construção dos conceitos de confiabilidade e autenticidade documental dentro do sistema.

  12. Impacto da introdução de mídia eletrônica num curso de patologia geral

    Directory of Open Access Journals (Sweden)

    Flávia Branco Cerqueira Serra Neves

    Full Text Available OBJETIVO: Avaliar modificações implementadas recentemente num curso de Patologia Geral (PG, com vistas a incentivar a auto-instrução dos alunos por meio de recursos eletrônicos. METODOLOGIA: Estudantes do curso de PG da Escola Bahiana de Medicina e Saúde Pública foram solicitados a preencher um questionário sobre modificações implementadas na disciplina, entre elas a utilização de um CD-ROM elaborado e distribuído pela disciplina. RESULTADOS: Foram avaliados 288 estudantes. A importância média dada ao CD-ROM foi de 8,45 ± 1,79, numa escala de 0 a 10, sendo esta a principal fonte de estudo utilizada por 97,2% dos estudantes para a prova prática. Os alunos que estudaram pelo CD apresentaram menor freqüência de notas baixas - consideradas inferiores a 6,0 (39,9% x 87,5%, P = 0,010 - e referiram conseguir identificar os processos gerais com maior freqüência (99,3% x 87,5%, P = 0,012 em relação aos que não utilizaram este método. 95,8% dos alunos acharam que o curso melhorou sua capacidade de reconhecer tecidos e órgãos ao microscópio. CONCLUSÕES: O CD-ROM utilizado oferece recursos que podem facilitar o processo ensino-aprendizagem, representando uma alternativa para o ensino integrado de PG.

  13. Igrejas eletrônicas: espetacularização e consumo de bens simbólicos no neopentecostalismoicos no neopentecostalismo

    Directory of Open Access Journals (Sweden)

    Roger Bressianini Almeida

    2016-05-01

    Full Text Available O artigo apresenta uma análise das práticas espetaculares e do consumo de bens simbólicos nas igrejas eletrônicas. Dentro do modelo neopentecostal iniciado no final do século XX, estratégias são lançadas para a construção de imagens midiatizadas e passíveis de veneração, personificadas na figura dos líderes de suas denominações. Com este processo realizado por meio das práticas de espetacularização descritas por Guy Debord, os líderes religiosos realizam uma transferência de seu poder carismático para pequenos bens simbólicos desprovidos de valor de uso que, uma vez consumidos, materializam os poderes simbólicos e econômicos que possibilitam a manutenção e expansão de suas instituições. Partindo do conceito de campo religioso proposto por Pierre Bourdieu, este estudo aborda peculiaridades e problemas de um modelo de religião em que as imagens midiáticas adquirem conceito de imagens de culto. Por apresentar altas taxas de crescimento de adeptos no Brasil nas últimas décadas, torna-se necessário compreender e ampliar os conhecimentos a respeito desses movimentos religiosos e suas estratégias de operação.

  14. O “animal eletrônico”: dilemas do ciberativismo verdeThe ‘electronic animal’: dilemmas of green cyberactivism

    Directory of Open Access Journals (Sweden)

    Samira Feldman Marzochi

    Full Text Available Combinando as principais contribuições teóricas do Estruturalismo, Fenomenologia e Teoria Crítica, este artigo parte do pressuposto de que a vulgarização do termo “rede” tem contribuído para um entendimento apologético de fenômenos contemporâneos relacionados ao tema da política e da comunicação, incluindo-se a prática do “ciberativismo”. No intuito de retirar a carga ideológica do conceito, o artigo pincela as mudanças nas relações entre indivíduo e esfera pública desde a Grécia antiga, passando pela industrialização, até deter-se na modernidade contemporânea. A partir desta contextualização teórica e histórica, foi possível analisar criticamente a correspondência, presente no senso comum, entre “ciberespaço” e esfera pública, “ciberativista” e sujeito político, bem como apontar a virtualização da natureza e das questões ambientais, perpetrada pelas campanhas de organizações não governamentais. Assim, é apresentado o conceito de “animal eletrônico” como alegoria de uma “antipolítica”, da ausência da dimensão política que caracteriza os homens, os distingue entre si e do r eino natural.

  15. Aprendizagem Significativa e o Portfólio Reflexivo Eletrônico na Educação Médica

    Directory of Open Access Journals (Sweden)

    Mirian Vieira Maia

    Full Text Available RESUMO A pesquisa-intervenção analisou o uso do portfólio reflexivo eletrônico (PRE na educação médica, no contexto do internato em Anestesiologia, pela perspectiva da Teoria da Aprendizagem Significativa. Objetivos Investigar o papel do PRE na prática reflexiva e no processo de aprendizagem; identificar as percepções dos alunos sobre ele e como as tecnologias de informação e comunicação (TICs contribuíram nesse processo. A tipologia utilizada na investigação da aprendizagem significativa (AS baseia-se na avaliação das articulações conceituais feitas pelos alunos para representar e qualificar seus conhecimentos. Resultados 60% dos alunos referiram refletir ao elaborar o PRE e documentaram reflexão em suas narrativas; os portfólios de todos os alunos possibilitaram a recursividade; 60% dos alunos evidenciaram AS em suas narrativas; foi possível conhecer o processo individual de elaboração do PRE, os aspectos positivos e limitantes em seu uso pedagógico, e as contribuições das TICs nesse processo. Esses resultados sugerem que o PRE favorece e estimula a reflexão, facilitando o processo de AS e contribuindo no acompanhamento contínuo do aprendizado consolidado pelo aluno, não só no internato em Anestesiologia, como em outros contextos essencialmente práticos.

  16. Comunidade de marca ou boca a boca eletrônico: qual o objetivo da presença de empresas em mídias sociais?

    Directory of Open Access Journals (Sweden)

    Michel Lens Seller

    2018-02-01

    Full Text Available Resumo O uso crescente de mídias sociais vem alterando o relacionamento entre empresas e clientes. As mídias sociais abrem um canal de comunicação bidirecional entre a organização e seus consumidores, assim como promove a interação entre estes. De acordo com a literatura, são dois os objetivos perseguidos pelas empresas neste ambiente: aumento de confiança e lealdade à marca por meio de comunidades de marca; e influência na decisão de compra do cliente pela promoção de comunicação boca a boca positiva sobre seus produtos. O presente artigo estuda a abordagem utilizada por duas empresas (dos segmentos farmacêutico e de varejo de moda em suas fan pages no Facebook. Evidências de consciência comum e prática de rituais e tradições obtidas a partir de um estudo netnográfico revelam uma comunidade de marca na página da empresa farmacêutica. A análise de métricas de boca a boca eletrônico revela que o estímulo a esta forma de comunicação é o que busca a indústria de moda por meio de sua página. O estudo ainda discute mecanismos para estimular o boca a boca em fan pages e a presença de vínculos fracos como fator de engajamento de consumidores nesta prática. Implicações gerenciais, limitações e sugestões de pesquisas futuras são apresentadas ao final.

  17. A Model for the Sounding Rocket Measurement on an Ionospheric E-F Valley at the Hainan Low Latitude Station

    International Nuclear Information System (INIS)

    Wang Zheng; Shi Jiankui; Guan Yibing; Liu Chao; Zhu Guangwu; Torkar Klaus; Fredrich Martin

    2014-01-01

    To understand the physics of an ionospheric E-F valley, a new overlapping three-Chapman-layer model is developed to interpret the sounding rocket measurement in the morning (sunrise) on May 7, 2011 at the Hainan low latitude ionospheric observation station (19.5°N, 109.1°E). From our model, the valley width, depth and height are 43.0 km, 62.9% and 121.0 km, respectively. From the sounding rocket observation, the valley width, depth and height are 42.2 km, 47.0% and 123.5 km, respectively. The model results are well consistent with the sounding rocket observation. The observed E-F valley at Hainan station is very wide and deep, and rapid development of the photochemical process in the ionosphere should be the underlying reason. (astrophysics and space plasma)

  18. In vivo colocalization of 2-nitroimidazole EF5 fluorescence intensity and electron paramagnetic resonance oximetry in mouse tumors

    International Nuclear Information System (INIS)

    Mahy, Pierre; Bast, Marc de; Gallez, Bernard; Gueulette, John; Koch, Cameron J.; Scalliet, Pierre; Gregoire, Vincent

    2003-01-01

    Background and purpose: The primary objective of this study was to establish in vivo the relationship between 2-2-nitro-1H-imidazol-1yl-N-(2,2,3,3,3-pentafluoropropyl)-acetamide (EF5) adduct formation and intratumoral oxygen concentrations measured by electron paramagnetic resonance (EPR) in a tumor model mimicking a clinical situation. The secondary objective was an attempt to calibrate in situ the immunofluorescence (IF) signal with EPR oximetry. Materials and methods: IM syngeneic fibrosarcoma (NFSA) bearing C3H mice were used. Three days after injection of a paramagnetic charcoal into the tumor, the mice were anesthetized, injected with the hypoxic marker EF5, and monitored every 20 min for 3 h with a low-frequency EPR spectrometer. Animals were allowed to breath either under 21 or 100% O 2 . Tumors were then harvested, frozen, cut into sections including the charcoal and processed for EF5 adducts detection using monoclonal antibodies. Slices were viewed with a fluorescence microscope and 190x140 μm areas surrounding the charcoal were digitized and analyzed with the NIH-Image and Adobe Photoshop TM software. The fluorescence intensity (FI) was measured in the whole pictures and in strips of 10 μm around the charcoal. Results: EF5 binding increased with decreasing pO 2 , most substantially at pO 2 below 5 mm Hg. Baseline (ambient air) pO 2 reached 3.2±2.1 mm Hg in NFSA tumors. It increased to 9.8±3.2 mm Hg under 100% O 2 . A statistically significant correlation was observed on an individual tumor basis between the FI in the first 10 μm strip around the charcoal and the pO 2 determined by EPR oximetry (Wilcoxon signed rank test: P 2 in an in vivo environment under biologically-relevant pO 2 values of less than 10 mm Hg

  19. Thermostability of Multidomain Proteins: Elongation Factors EF-Tu from Escherichia coli and Bacillus stearothermophilus and Their Chimeric Forms

    Czech Academy of Sciences Publication Activity Database

    Šanderová, Hana; Hůlková, Marta; Maloň, Petr; Kepková, M.; Jonák, Jiří

    2004-01-01

    Roč. 13, č. 1 (2004), s. 89-99 ISSN 0961-8368 R&D Projects: GA AV ČR IPP1050128; GA ČR GA204/98/0863; GA ČR GA303/02/0689 Institutional research plan: CEZ:AV0Z4055905; CEZ:AV0Z5052915 Keywords : elongation factor EF-Tu, thermostability, chimeric protein Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.116, year: 2004

  20. Consumption and the Consumer

    Directory of Open Access Journals (Sweden)

    Maria VADUVA

    2016-09-01

    Full Text Available The consumer is that trader responsible for consumption act of some final goods or services who decided what must be produce and in what cantity, being the one who make the economic mechanism to move. Consumption is in close connection with the production of goods and services, exerting an active role, any activity should be complete by consuming its results; consumption creates the motivation to achieve economic and non-economic activities. The traditional approach to consumer behavior starts from hypothesised that all consumers seek to maximize the aggregate utility obtained of satisfactions resulting from consumption of goods taking into account the budgetary constraints given by income that consumer has and the prices of these goods. In the conditions of modern economy, consumption can be increased by diseconomies. If consumption depends on permanent income, revenue growth effectively does not exert influence on consumption only to the extent that this increase of income leads to increasing permanent income consumer. Consumption is viewed as an active agent of economic life, it is not only a consumer of goods and services but also a producer.

  1. Experience and Sustainable Consumption

    DEFF Research Database (Denmark)

    Rasmussen, Tove Arendt

    consumption may re-enchant ordinary consumption and thereby even become a part of marketing and the experience economy. New layers of meaning are at stake and altruistic motives come into play; doing something good for someone or something, aside from oneself, is a very strong trigger of positive emotions...

  2. Experience and Sustainable Consumption

    DEFF Research Database (Denmark)

    Rasmussen, Tove Arendt

    2014-01-01

    consumption may re-enchant ordinary consumption and thereby even become a part of marketing and the experience economy. New layers of meaning are at stake and altruistic motives come into play; doing something good for someone or something, aside from oneself, is a very strong trigger of positive emotions...

  3. Value Creation and Consumption

    DEFF Research Database (Denmark)

    Taillard, Marie; Voyer, Benjamin; Glaveanu, Vlad Petre

    2014-01-01

    An effect of the digital economy has been to provide consumers with almost unlimited ways to customize their consumption activities. They can craft each consumption experience to fit specific contexts, needs, emotions and other factors. A teenager’s night out is documented and shared with her...

  4. Identification of novel mazEF/pemIK family toxin-antitoxin loci and their distribution in the Staphylococcus genus.

    Science.gov (United States)

    Bukowski, Michal; Hyz, Karolina; Janczak, Monika; Hydzik, Marcin; Dubin, Grzegorz; Wladyka, Benedykt

    2017-10-18

    The versatile roles of toxin-antitoxin (TA) systems in bacterial physiology and pathogenesis have been investigated for more than three decades. Diverse TA loci in Bacteria and Archaea have been identified in genome-wide studies. The advent of massive parallel sequencing has substantially expanded the number of known bacterial genomic sequences over the last 5 years. In staphylococci, this has translated into an impressive increase from a few tens to a several thousands of available genomes, which has allowed us for the re-evalution of prior conclusions. In this study, we analysed the distribution of mazEF/pemIK family TA system operons in available staphylococcal genomes and their prevalence in mobile genetic elements. 10 novel m azEF/pemIK homologues were identified, each with a corresponding toxin that plays a potentially different and undetermined physiological role. A detailed characterisation of these TA systems would be exceptionally useful. Of particular interest are those associated with an SCCmec mobile genetic element (responsible for multidrug resistance transmission) or representing the joint horizontal transfer of TA systems and determinants of vancomycin resistance from enterococci. The involvement of TA systems in maintaining mobile genetic elements and the associations between novel mazEF/pemIK loci and those which carry drug resistance genes highlight their potential medical importance.

  5. Elucidating the Role of Injury-Induced Electric Fields (EFs in Regulating the Astrocytic Response to Injury in the Mammalian Central Nervous System.

    Directory of Open Access Journals (Sweden)

    Matthew L Baer

    Full Text Available Injury to the vertebrate central nervous system (CNS induces astrocytes to change their morphology, to increase their rate of proliferation, and to display directional migration to the injury site, all to facilitate repair. These astrocytic responses to injury occur in a clear temporal sequence and, by their intensity and duration, can have both beneficial and detrimental effects on the repair of damaged CNS tissue. Studies on highly regenerative tissues in non-mammalian vertebrates have demonstrated that the intensity of direct-current extracellular electric fields (EFs at the injury site, which are 50-100 fold greater than in uninjured tissue, represent a potent signal to drive tissue repair. In contrast, a 10-fold EF increase has been measured in many injured mammalian tissues where limited regeneration occurs. As the astrocytic response to CNS injury is crucial to the reparative outcome, we exposed purified rat cortical astrocytes to EF intensities associated with intact and injured mammalian tissues, as well as to those EF intensities measured in regenerating non-mammalian vertebrate tissues, to determine whether EFs may contribute to the astrocytic injury response. Astrocytes exposed to EF intensities associated with uninjured tissue showed little change in their cellular behavior. However, astrocytes exposed to EF intensities associated with injured tissue showed a dramatic increase in migration and proliferation. At EF intensities associated with regenerating non-mammalian vertebrate tissues, these cellular responses were even more robust and included morphological changes consistent with a regenerative phenotype. These findings suggest that endogenous EFs may be a crucial signal for regulating the astrocytic response to injury and that their manipulation may be a novel target for facilitating CNS repair.

  6. Elucidating the Role of Injury-Induced Electric Fields (EFs) in Regulating the Astrocytic Response to Injury in the Mammalian Central Nervous System.

    Science.gov (United States)

    Baer, Matthew L; Henderson, Scott C; Colello, Raymond J

    2015-01-01

    Injury to the vertebrate central nervous system (CNS) induces astrocytes to change their morphology, to increase their rate of proliferation, and to display directional migration to the injury site, all to facilitate repair. These astrocytic responses to injury occur in a clear temporal sequence and, by their intensity and duration, can have both beneficial and detrimental effects on the repair of damaged CNS tissue. Studies on highly regenerative tissues in non-mammalian vertebrates have demonstrated that the intensity of direct-current extracellular electric fields (EFs) at the injury site, which are 50-100 fold greater than in uninjured tissue, represent a potent signal to drive tissue repair. In contrast, a 10-fold EF increase has been measured in many injured mammalian tissues where limited regeneration occurs. As the astrocytic response to CNS injury is crucial to the reparative outcome, we exposed purified rat cortical astrocytes to EF intensities associated with intact and injured mammalian tissues, as well as to those EF intensities measured in regenerating non-mammalian vertebrate tissues, to determine whether EFs may contribute to the astrocytic injury response. Astrocytes exposed to EF intensities associated with uninjured tissue showed little change in their cellular behavior. However, astrocytes exposed to EF intensities associated with injured tissue showed a dramatic increase in migration and proliferation. At EF intensities associated with regenerating non-mammalian vertebrate tissues, these cellular responses were even more robust and included morphological changes consistent with a regenerative phenotype. These findings suggest that endogenous EFs may be a crucial signal for regulating the astrocytic response to injury and that their manipulation may be a novel target for facilitating CNS repair.

  7. Characterization of the 1st and 2nd EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism

    Directory of Open Access Journals (Sweden)

    Wei Chin-Chuan

    2012-04-01

    Full Text Available Abstract Background Superoxide generated by non-phagocytic NADPH oxidases (NOXs is of growing importance for physiology and pathobiology. The calcium binding domain (CaBD of NOX5 contains four EF-hands, each binding one calcium ion. To better understand the metal binding properties of the 1st and 2nd EF-hands, we characterized the N-terminal half of CaBD (NCaBD and its calcium-binding knockout mutants. Results The isothermal titration calorimetry measurement for NCaBD reveals that the calcium binding of two EF-hands are loosely associated with each other and can be treated as independent binding events. However, the Ca2+ binding studies on NCaBD(E31Q and NCaBD(E63Q showed their binding constants to be 6.5 × 105 and 5.0 × 102 M-1 with ΔHs of -14 and -4 kJ/mol, respectively, suggesting that intrinsic calcium binding for the 1st non-canonical EF-hand is largely enhanced by the binding of Ca2+ to the 2nd canonical EF-hand. The fluorescence quenching and CD spectra support a conformational change upon Ca2+ binding, which changes Trp residues toward a more non-polar and exposed environment and also increases its α-helix secondary structure content. All measurements exclude Mg2+-binding in NCaBD. Conclusions We demonstrated that the 1st non-canonical EF-hand of NOX5 has very weak Ca2+ binding affinity compared with the 2nd canonical EF-hand. Both EF-hands interact with each other in a cooperative manner to enhance their Ca2+ binding affinity. Our characterization reveals that the two EF-hands in the N-terminal NOX5 are Ca2+ specific. Graphical abstract

  8. Evidence of MexT-independent overexpression of MexEF-OprN multidrug efflux pump of Pseudomonas aeruginosa in presence of metabolic stress.

    Directory of Open Access Journals (Sweden)

    Ayush Kumar

    Full Text Available The Pseudomonas aeruginosa MexEF-OprN efflux pump confers resistance to clinically significant antibiotics. Regulation of mexEF-oprN operon expression is multifaceted with the MexT activator being one of the most prominent regulatory proteins.We have exploited the impaired metabolic fitness of a P. aeruginosa mutant strain lacking several efflux pump of the resistance nodulation cell division superfamily and the TolC homolog OpmH, and isolated derivatives (large colony variants that regained fitness by incubation on nutrient-rich medium in the absence of antibiotics. Although the mexEF-oprN operon is uninducible in this mutant due to a 8-bp mexT insertion present in some P. aeruginosa PAO1 strains, the large colony variants expressed high levels of MexEF-OprN. Unlike large colony variants obtained after plating on antibiotic containing medium which expressed mexEF-oprN in a MexT-dependent fashion as evidenced by clean excision of the 8-bp insertion from mexT, mexEF-oprN expression was MexT-independent in the large colony variants obtained by plating on LB alone since the mexT gene remained inactivated. A search for possible regulators of mexEF-oprN expression using transposon mutagenesis and genomic library expression approaches yielded several candidates but proved inconclusive.Our results show that antibiotic and metabolic stress lead to up-regulation of MexEF-OprN expression via different mechanisms and that MexEF-OprN does not only extrude antimicrobials but rather serves other important metabolic functions.

  9. Modulating uranium binding affinity in engineered calmodulin EF-hand peptides: effect of phosphorylation.

    Directory of Open Access Journals (Sweden)

    Romain Pardoux

    Full Text Available To improve our understanding of uranium toxicity, the determinants of uranyl affinity in proteins must be better characterized. In this work, we analyzed the contribution of a phosphoryl group on uranium binding affinity in a protein binding site, using the site 1 EF-hand motif of calmodulin. The recombinant domain 1 of calmodulin from A. thaliana was engineered to impair metal binding at site 2 and was used as a structured template. Threonine at position 9 of the loop was phosphorylated in vitro, using the recombinant catalytic subunit of protein kinase CK2. Hence, the T(9TKE(12 sequence was substituted by the CK2 recognition sequence TAAE. A tyrosine was introduced at position 7, so that uranyl and calcium binding affinities could be determined by following tyrosine fluorescence. Phosphorylation was characterized by ESI-MS spectrometry, and the phosphorylated peptide was purified to homogeneity using ion-exchange chromatography. The binding constants for uranyl were determined by competition experiments with iminodiacetate. At pH 6, phosphorylation increased the affinity for uranyl by a factor of ∼5, from K(d = 25±6 nM to K(d = 5±1 nM. The phosphorylated peptide exhibited a much larger affinity at pH 7, with a dissociation constant in the subnanomolar range (K(d = 0.25±0.06 nM. FTIR analyses showed that the phosphothreonine side chain is partly protonated at pH 6, while it is fully deprotonated at pH 7. Moreover, formation of the uranyl-peptide complex at pH 7 resulted in significant frequency shifts of the ν(as(P-O and ν(s(P-O IR modes of phosphothreonine, supporting its direct interaction with uranyl. Accordingly, a bathochromic shift in ν(as(UO(2(2+ vibration (from 923 cm(-1 to 908 cm(-1 was observed upon uranyl coordination to the phosphorylated peptide. Together, our data demonstrate that the phosphoryl group plays a determining role in uranyl binding affinity to proteins at physiological pH.

  10. Modulating uranium binding affinity in engineered Calmodulin EF-hand peptides: effect of phosphorylation

    International Nuclear Information System (INIS)

    Pardoux, Romain; Sauge-Merle, Sandrine; Lemaire, David; Guilloreau, Luc; Berthomieu, Catherine; Delangle, Pascale; Adriano, Jean-Marc

    2012-01-01

    To improve our understanding of uranium toxicity, the determinants of uranyl affinity in proteins must be better characterized. In this work, we analyzed the contribution of a phosphoryl group on uranium binding affinity in a protein binding site, using the site 1 EF-hand motif of calmodulin. The recombinant domain 1 of calmodulin from A. thaliana was engineered to impair metal binding at site 2 and was used as a structured template. Threonine at position 9 of the loop was phosphorylated in vitro, using the recombinant catalytic subunit of protein kinase CK2. Hence, the T 9 TKE 12 sequence was substituted by the CK2 recognition sequence TAAE. A tyrosine was introduced at position 7, so that uranyl and calcium binding affinities could be determined by following tyrosine fluorescence. Phosphorylation was characterized by ESI-MS spectrometry, and the phosphorylated peptide was purified to homogeneity using ion-exchange chromatography. The binding constants for uranyl were determined by competition experiments with iminodiacetate. At pH 6, phosphorylation increased the affinity for uranyl by a factor of ∼5, from K d =25±6 nM to K d =5±1 nM. The phosphorylated peptide exhibited a much larger affinity at pH 7, with a dissociation constant in the sub-nanomolar range (K d = 0.25±0.06 nM). FTIR analyses showed that the phospho-threonine side chain is partly protonated at pH 6, while it is fully deprotonated at pH 7. Moreover, formation of the uranyl-peptide complex at pH 7 resulted in significant frequency shifts of the ν as (P-O) and ν s (P-O) IR modes of phospho-threonine, supporting its direct interaction with uranyl. Accordingly, a bathochromic shift in ν as (UO 2 ) 2+ vibration (from 923 cm -1 to 908 cm -1 ) was observed upon uranyl coordination to the phosphorylated peptide. Together, our data demonstrate that the phosphoryl group plays a determining role in uranyl binding affinity to proteins at physiological pH. (authors)

  11. Blood donors--Serious adverse reactions (SAR) 2010-2014 EFS Châteauroux, France.

    Science.gov (United States)

    Riga, A; Sapey, T; Bacanu, M; Py, J-Y; Dehaut, F

    2015-06-01

    In 2013, the national French incidence of serious adverse reactions (SAR) was 155.7 per 100,000 donations and 82% of SAR were grade 2 (French classification of SAR related to blood donors) The purpose of our study was to describe the profile of blood donator candidate which had a SAR in our center. The study contains all the SAR superior to grade 1 occurred on the site EFS Châteauroux (site and mobile blood collection) from January 2010 to October 31, 2014. We analyzed 37 parameters from the e-fit files (e-site French blood vigilance) and In-log software. We identified 82 SAR for 72,553 blood donations (incidence: 113.02 SAR per 100,000 donations). Forty-one men and 41 women, middle age 39 years (18-66). Average height: 1.68 m (1.49-1.85); average weight: 68 kg (50-98); body mass index (kg/m(2)): 24,13(18.6-31.9). All donors were Caucasian and 30% unemployed. We found 74 vasovagal syncope (VVS), 5 hematomas, 2 arterial injuries and an adverse reaction to citrate. In 90%, the SAR was immediate and of grade 2 in 85% of cases. Thirty-seven percent of SAR were first donation in connection with whole blood in 87% of cases. Regarding the seniority of donors, the number of average donations (whole blood, plasma, platelets) was 16.5. An SAR determined the stop of blood donation in 65% of cases with nearly 80% stoppage if it was a first donation. Seventy-three percent of SAR as a VVS took place during blood collection or within 5 minutes following the end of the donation. Sixty-one percent were men. Forty-four percent of cases were a first donation and 83% occurred in mobile blood collection. Average age was 36 years. The result was a permanent stop of all type of donations in 76% of cases. Twenty-seven percent of SAR as a VVS took place beyond 5 minutes after the end of the donation. Seventy-five percent were women. Thirty percent of cases were a first donation and 95% of SAR occurred in mobile blood collection. Average age was 42 years. The result was a permanent stop of

  12. The fsr Quorum-Sensing System and Cognate Gelatinase Orchestrate the Expression and Processing of Proprotein EF_1097 into the Mature Antimicrobial Peptide Enterocin O16

    OpenAIRE

    Dundar, Halil; Brede, Dag A.; La Rosa, Sabina Leanti; El-Gendy, Ahmed Osama; Diep, Dzung B.; Nes, Ingolf F.

    2015-01-01

    A novel antimicrobial peptide designated enterocin O16 was purified from Enterococcus faecalis. Mass spectrometry showed a monoisotopic mass of 7,231 Da, and N-terminal Edman degradation identified a 29-amino-acid sequence corresponding to residues 90 to 119 of the EF_1097 protein. Bioinformatic analysis showed that enterocin O16 is composed of the 68 most C-terminal residues of the EF_1097 protein. Introduction of an in-frame isogenic deletion in the ef1097 gene abolished the production of e...

  13. Bat consumption in Thailand

    Directory of Open Access Journals (Sweden)

    Kanokwan Suwannarong

    2016-01-01

    Full Text Available Background: Human consumption of bats poses an increasing public health threat globally. Communities in which bat guano is mined from caves have extensive exposure to bat excreta, often harvest bats for consumption, and are at risk for bat-borne diseases. Methods: This rapid ethnographic study was conducted in four provinces of Thailand (Ratchaburi, Sakaeo, Nakorn Sawan, and Phitsanulok, where bat guano was mined and sold during the period April–August 2014. The aim of this study was to understand behaviors and risk perceptions associated with bat conservation, exposure to bats and their excreta, and bat consumption. Sixty-seven respondents playing various roles in bat guano mining, packaging, sale, and use as fertilizer participated in the study. Data were collected through interviews and/or focus group discussions. Results: In spite of a bat conservation program dating back to the 1980s, the benefits of conserving bats and the risks associated with bat consumption were not clear and infrequently articulated by study respondents. Discussion: Since bat consumption continues, albeit covertly, the risk of bat-borne diseases remains high. There is an opportunity to reduce the risk of bat-borne diseases in guano-mining communities by strengthening bat conservation efforts and raising awareness of the health risks of bat consumption. Further research is suggested to test behavior change strategies for reducing bat consumption.

  14. Bat consumption in Thailand.

    Science.gov (United States)

    Suwannarong, Kanokwan; Schuler, Sidney

    2016-01-01

    Human consumption of bats poses an increasing public health threat globally. Communities in which bat guano is mined from caves have extensive exposure to bat excreta, often harvest bats for consumption, and are at risk for bat-borne diseases. This rapid ethnographic study was conducted in four provinces of Thailand (Ratchaburi, Sakaeo, Nakorn Sawan, and Phitsanulok), where bat guano was mined and sold during the period April-August 2014. The aim of this study was to understand behaviors and risk perceptions associated with bat conservation, exposure to bats and their excreta, and bat consumption. Sixty-seven respondents playing various roles in bat guano mining, packaging, sale, and use as fertilizer participated in the study. Data were collected through interviews and/or focus group discussions. In spite of a bat conservation program dating back to the 1980s, the benefits of conserving bats and the risks associated with bat consumption were not clear and infrequently articulated by study respondents. Since bat consumption continues, albeit covertly, the risk of bat-borne diseases remains high. There is an opportunity to reduce the risk of bat-borne diseases in guano-mining communities by strengthening bat conservation efforts and raising awareness of the health risks of bat consumption. Further research is suggested to test behavior change strategies for reducing bat consumption.

  15. Marriage and Consumption

    DEFF Research Database (Denmark)

    Blow, Laura; Browning, Martin; Ejrnæs, Mette

    We examine theoretically and empirically consumption over the early part of the life-cycle. The main focus is on the transition from being single to living with someone else. Our theoretical model allows for publicness in consumption; uncertainty concerning marriage; differences between lifetime...... incomes for prospective partners and a marriage premium. We develop a two period model to bring out the main features of the impact of marriage on consumption and saving. We then develop a multi-period model that can be taken to the data on expenditures by singles and couples aged between 18 and 30. Our...

  16. Scripts de atendimento em call centers: uma visão de documentos eletrônicosScript for call center attendance: a view on electronic documents

    Directory of Open Access Journals (Sweden)

    Sandra Maria Silveira

    2010-05-01

    Full Text Available O presente artigo contextualiza o serviço de atendimento aos clientes (SAC no âmbito da Ciência da Informação e busca compreender o fenômeno sob o enfoque da organização e do uso da informação. Analisa-se o processo informacional dos serviços de atendimento aos clientes realizado em call centers, com o foco na organização e no uso das bases de informação, tendo em vista a sistematização de parâmetros para subsidiarem a criação, a manutenção e a validação dos scripts de atendimento. Scripts são documentos eletrônicos que contêm orientação para executar procedimentos relativos a produtos e serviços da instituição e na interação com os consumidores. Por fim, identificaram-se os atributos de qualidade de informação que orientam a construção e a manutenção das bases de informações e sistematizaram-se os parâmetros para elaboração das bases de informações em consonância com os processos de organização e uso da informação no âmbito do SAC.The present article contextualizes the service of attendance to clients (SAC as far as the Information Science is concerned. It also seeks to understand the phenomenon under the focus of organization and the use of information. We analyze the informational process of attendance services to clients performed in call centers, focusing on the organization and the use of information bases. This is done aiming at the systematization of parameters to subside the creation, maintenance and validation of scripts for attendance. Scripts are electronic documents that contain orientation to execute procedures related to products and services of the institution and in the interaction with consumers. At last we identify the attributes of quality of information that guide the construction and maintenance of information bases and systematize the parameters for elaboration of information bases according to organization processes and use of information at SAC.

  17. Governo eletrônico e capital intelectual nas organizações públicas Electronic government and human capital in public organizations

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Joia

    2009-12-01

    Full Text Available Este artigo mostra a variação de capital intelectual em organizações públicas envolvidas em empreendimentos government-to-government (G2G. Para tanto, um frame heurístico foi desenvolvido a fim de avaliar o impacto de projetos G2G nos componentes do capital intelectual de agências públicas, isto é, em seus capitais humano, organizacional, de relacionamento e de inovação. Aplicando-se esse frame heurístico a 30 empreendimentos G2G no Brasil, ligando o Banco Central com os tribunais de justiça, detectou-se um impacto positivo no capital intelectual das organizações envolvidas nesses empreendimentos. Além disso, verificou-se que tais empreendimentos G2G influenciaram os componentes do capital intelectual de forma homogênea. A partir daí, o frame foi validado por meio de uma elevada e positiva correlação entre a variação do capital intelectual nas organizações públicas e a percepção de valor dos funcionários envolvidos nesses mesmos empreendimentos. Finalmente, adicionalmente às limitações da pesquisa, algumas conclusões são apresentadas a gestores públicos, practitioners e acadêmicos, para que seja entendido o potencial de uso da teoria do capital intelectual para avaliação de iniciativas de governo eletrônicoThis article shows the intellectual capital variation in public organizations involved in government-to-government (G2G enterprises. A heuristic frame was developed to measure the impact of G2G projects on the intellectual capital components of public agencies, i.e., on their human, organizational, relational and innovative capitals. This heuristic frame was applied to 30 G2G enterprises in Brazil connecting the Central Bank to the courts of justice and revealed a positive impact on the intellectual capital of the organizations involved. Furthermore, these G2G enterprises influenced the intellectual capital components uniformly. Then the frame was validated by means of a high and positive correlation

  18. O Comércio Eletrônico, os métodos de pagamentos e os mecanismos de segurança

    Directory of Open Access Journals (Sweden)

    Robson Antonio Moreira

    2016-10-01

    Full Text Available O objetivo deste artigo é apresentar os conceitos sobre comércio eletrônico, reconhecido mundialmente como e-commerce. O artigo apresenta os conceitos sobre essa forma de comércio, suas características e distinções quanto ao meio de transação comercial. Embora o termo e-commerce seja utilizado para representar toda transação por meio das redes de computadores e internet, há uma distinção e uma classificação diante dos envolvidos que podem ser clientes, fornecedores e até mesmo órgãos governamentais. Indicadores apresentados neste trabalho comprovam que essa modalidade traz comodidades aos clientes e desafios aos comerciantes. Sua participação aumenta gradativamente não apenas em usuários de computadores do tipo desktop, mas também nos smartphones. Mas, essa comodidade gera preocupações, sobretudo sobre a segurança da informação e o receio de fraudes nessas transações. Esses são os grandes temores que devem ser minimizados. Com o objetivo de garantir essa segurança, os pagamentos em transações de e-commerce, podem ser realizados de várias maneiras, sempre com o objetivo de reforçar a segurança nas informações que estão trafegando pela Internet. O presente artigo conclui que as empresas que praticam o e-commerce oferecem uma variedade opções para a efetivação da compra e, estas opções possuem garantias de segurança através de recursos da tecnologia da informação. Porém, mesmo com a eficiência da segurança na tecnologia é indispensável que o cliente faça uso consciente desta modalidade de comércio porque muitos dos problemas ocorrem por erros e más práticas do usuário.

  19. Interactive energy consumption visualization

    CSIR Research Space (South Africa)

    Lunga, D

    2014-11-01

    Full Text Available in an office building environment. The main goal is to highlight high consumptions patterns, estimate costs and savings, and recommend energy saving strategies. In its useful nature, the dashboard can provide valuable information for further programs tied...

  20. Cross-cultural adaptation and validity of the "Edmonton Frail Scale - EFS" in a Brazilian elderly sample Adaptación cultural y validez de la Edmonton Frail Scale - EFS en una muestra de ancianos brasileños Adaptação cultural e validade da Edmonton Frail Scale - EFS em uma amostra de idosos brasileiros

    Directory of Open Access Journals (Sweden)

    Suzele Cristina Coelho Fabrício-Wehbe

    2009-12-01

    Full Text Available This study aimed to assess the cross-cultural adaptation of the Edmonton Frail Scale (EFS and its validity in a Brazilian elderly sample. Translation and back-translation were performed, as well as discussion with professionals and elderly for conceptual equivalence, semantic validation and pre-test of the scale. The scale was applied to 137 elderly aged 65 years or older who lived in the community. In the know-groups validation of the frailty diagnosis between gender, age and cognitive deficit, elder elderly, female and with a cognitive deficit scored higher on the frailty diagnosis. A negative convergent correlation was found between the EFS and the Functional Independence Measure (FIM (-0.53, pEl objetivo de este estudio fue evaluar la adaptación cultural de la Edmonton Frail Scale (EFS y su validez en una muestra de ancianos brasileños. Fueron realizadas las etapas de traducción y retrotraducción, discusión con profesionales y ancianos para equivalencia conceptual, validación semántica y prueba piloto de la escala. El instrumento fue aplicado en 137 ancianos, con 65 años o más de edad, que vivían en la comunidad. En la validación de grupos conocidos, del diagnóstico de fragilidad entre sexo, edad y déficit cognitivo, los ancianos con edad más avanzada, sexo femenino y con déficit cognitivo tuvieron mayor puntuación en el diagnóstico de fragilidad. Hubo una correlación convergente negativa de la EFS con la Medida de Independencia Funcional (MIF (-0,53, pO objetivo deste estudo foi avaliar a adaptação cultural da Edmonton Frail Scale (EFS e sua validade em uma amostra de idosos brasileiros. Foram realizadas as etapas de tradução e retrotradução, discussão com profissionais e idosos para equivalência conceitual, validação semântica e pré-teste da escala. O instrumento foi aplicado em 137 idosos, com 65 anos ou mais de idade, que viviam na comunidade. Na validação de grupos conhecidos, do diagnóstico de

  1. Emotions and consumption behaviour

    OpenAIRE

    I. Soscia

    2013-01-01

    This stimulating book scrutinizes how emotions function in consumers, from both a psychological and a managerial perspective. It demonstrates how gratitude, happiness, guilt, anger, pride and sadness determine different post-consumption behaviours such as positive and negative word of mouth, repurchase intention and complaint behaviour. The emotional side of consumption seems to play an essential role in explaining choices made and actions taken by consumers. Isabella Soscia explores the ...

  2. Carbon offsetting: sustaining consumption?

    OpenAIRE

    Heather Lovell; Harriet Bulkeley; Diana Liverman

    2009-01-01

    In this paper we examine how theories of sustainable and ethical consumption help us to understand a new, rapidly expanding type of consumer product designed to mitigate climate change: carbon offsets. The voluntary carbon offset market grew by 200% between 2005 and 2006, and there are now over 150 retailers of voluntary carbon offsets worldwide. Our analysis concentrates on the production and consumption of carbon offsets, drawing on ideas from governmentality and political ecology about how...

  3. Changes in cultural consumption

    DEFF Research Database (Denmark)

    Navarrete, T.; Borowiecki, K. J.

    2016-01-01

    Visits to museums have been studied as hedonic and utilitarian forms of cultural consumption, though limited attention has been given to the access of museum collections online. We perform a unique historic analysis of the visibility of collections in a museum of ethnographic collections and comp......, particularly when showing them being used). Results support understanding of online heritage consumption and emerging dynamics, particularly outside of an institutional environment, such as Wikipedia. © 2016 Informa UK Limited, trading as Taylor & Francis Group....

  4. Consumption and Children

    DEFF Research Database (Denmark)

    Browning, Martin; Ejrnæs, Mette

    2009-01-01

    supply. We develop two tests of the extreme hypothesis that only changes in family structure matter. We estimate effects of the numbers and ages of children on consumption. These estimates allow us to rationalize all of the increase in consumption without recourse to any of the causal mechanisms. Our...... estimates can be interpreted either as giving upper bounds on the effects of children or as evidence that the other causes are not important....

  5. eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA Complex

    Directory of Open Access Journals (Sweden)

    José Manuel Mingot

    2013-11-01

    Full Text Available Exportin5 mediates the nuclear export of double-stranded RNAs, including pre-microRNAs, adenoviral RNAs, and tRNAs. When tRNAs are aminoacylated, the Exportin5-aminoacyl (aa-tRNA complex recruits and coexports the translation elongation factor eEF1A. Here, we show that eEF1A binds to Snail transcription factors when bound to their main target, the E-cadherin promoter, facilitating their export to the cytoplasm in association with the aa-tRNA-Exportin5 complex. Snail binds to eEF1A through the SNAG domain, a protein nuclear export signal present in several transcription factor families, and this binding is regulated by phosphorylation. Thus, we describe a nuclear role for eEF1A and provide a mechanism for protein nuclear export that attenuates the activity of SNAG-containing transcription factors.

  6. The fsr Quorum-Sensing System and Cognate Gelatinase Orchestrate the Expression and Processing of Proprotein EF_1097 into the Mature Antimicrobial Peptide Enterocin O16.

    Science.gov (United States)

    Dundar, Halil; Brede, Dag A; La Rosa, Sabina Leanti; El-Gendy, Ahmed Osama; Diep, Dzung B; Nes, Ingolf F

    2015-07-01

    A novel antimicrobial peptide designated enterocin O16 was purified from Enterococcus faecalis. Mass spectrometry showed a monoisotopic mass of 7,231 Da, and N-terminal Edman degradation identified a 29-amino-acid sequence corresponding to residues 90 to 119 of the EF_1097 protein. Bioinformatic analysis showed that enterocin O16 is composed of the 68 most C-terminal residues of the EF_1097 protein. Introduction of an in-frame isogenic deletion in the ef1097 gene abolished the production of enterocin O16. Enterocin O16 has a narrow inhibitory spectrum, as it inhibits mostly lactobacilli. Apparently, E. faecalis is intrinsically resistant to the antimicrobial peptide, as no immunity connected to the production of enterocin O16 could be identified. ef1097 has previously been identified as one of three loci regulated by the fsr quorum-sensing system. The introduction of a nonsense mutation into fsrB consistently impaired enterocin O16 production, but externally added gelatinase biosynthesis-activating pheromone restored the antimicrobial activity. Functional genetic analysis showed that the EF_1097 proprotein is processed extracellularly into enterocin O16 by the metalloprotease GelE. Thus, it is evident that the fsr quorum-sensing system constitutes the regulatory unit that controls the expression of the EF_1097 precursor protein and the protease GelE and that the latter is required for the formation of enterocin O16. On the basis of these results, this study identified antibacterial antagonism as a novel aspect related to the function of fsr and provides a rationale for why ef1097 is part of the fsr regulon. The fsr quorum-sensing system modulates important physiological functions in E. faecalis via the activity of GelE. The present study presents a new facet of fsr signaling. The system controls the expression of three primary target operons (fsrABCD, gelE-sprE, and ef1097-ef1097b). We demonstrate that the concerted expression of these operons constitutes the

  7. Economic theories of sustainable consumption

    NARCIS (Netherlands)

    Ferrer-i-Carbonell, Ada; Bergh, van den Jeroen C.J.M.

    1999-01-01

    The term `sustainable consumption' denotes the search for consumption patterns that reduce human pressure on the environment and nature. This searchinvolves three levels of research. First, the relationship between consumption, lifestyles and environmental sustainability has to be clarified.

  8. Compras na Administração Pública: o Pregão Eletrônico como Instrumento de Eficiência diante das Modalidades da Lei nº 8.666/93

    Directory of Open Access Journals (Sweden)

    André Lezan Fernandes

    2015-06-01

    Full Text Available O bom uso dos recursos públicos, a transparência dos processos de compras governamentais e o uso de novas tecnologias são elementos fundamentais para o bom funcionamento da máquina pública contemporânea. Este estudo tem por objetivo discorrer sobre a modalidade pregão eletrônico de licitações públicas. O método utilizado para a elaboração deste estudo foi baseado no estudo bibliográfico de obras existentes que tratam sobre o tema. A modalidade pregão surgiu no início dos anos 2000 como uma nova forma do ente público realizar suas aquisições; ela proporciona mais agilidade e eficiência nas compras públicas. Aliada a essa maior eficiência e agilidade na execução do processo licitatório, o pregão, principalmente sua variante eletrônica, possibilita maior facilidade de acesso às informações sobre os certames, facilitando a fiscalização e o controle sobre os gastos e despesas da administração pública.

  9. Gestão e segurança da informação eletrônica: Exigências para uma gestão documental eficaz no Brasil

    Directory of Open Access Journals (Sweden)

    Sandra Buth Zanon

    2015-04-01

    Full Text Available A gestão e segurança da informação, principal ativo das organizações, são questões imperativas na Era da Informação e do Conhecimento. A partir da prática na área de gestão documental e informacional em órgãos públicos do Brasil, observou-se a ausência de um modelo de gestão da informação eletrônica adequado às necessidades das organizações bem como de uma política de segurança da informação. Assim, apresenta-se uma reflexão sobre a necessidade premente de gestão das informações eletrônicas, visando a eficácia da gestão documental, e a importância da adoção de uma política de segurança da informação para assegurar sua integridade, confidencialidade, autenticidade, disponibilidade, não-repúdio e preservação a longo prazo.

  10. Enciclopédia eletrônica: semelhanças e diferenças com o documento tradicional 10.5007/1518-2924.2002v7n14p19

    Directory of Open Access Journals (Sweden)

    Magda Chagas Teixeira

    2002-01-01

    Full Text Available Acreditando que o estudo aprofundado dos documentos eletrônicos poderá contribuir para o seu aperfeiçoamento enquanto ferramentas importantes que são na disseminação e recuperação de informações, realiza-se, neste trabalho, uma análise do Almanaque Abril CD-ROM 98, procurando conhecer suas características e potencialidades, bem como suas semelhanças e diferenças com a enciclopédia tradicional. Para tanto, toma-se como base o trabalho desenvolvido por Hoey (1986 com relação às obras de referência, por ele denominadas de Colônias de Discursos. Após a análise, foi possível perceber que todas as características apresentadas pelo autor podem ser encontradas no Almanaque Abril CD-ROM 98, sendo que muitas delas aparecem de forma acentuada devido aos recursos oferecidos pela recuperação eletrônica de informações.

  11. Construção do conhecimento através das redes eletrônicas : o caso de uma escola especializada de ensino de 2 º grau: 1ª parte - os professores

    Directory of Open Access Journals (Sweden)

    Maria De Cléofas Faggion Alencar

    2001-01-01

    Full Text Available O estudo teve como objetivo mapear, através do hipertexto a ocorrência de acessos às informações na Web de alunos com relação às disciplinas de Biologia e Física do currículo de uma escola especializada de 2o grau e, em segundo lugar, caracterizar a atuação dos professores dessas mesmas áreas no uso da Internet. Nesse artigo, apresenta-se os resultados da segunda parte, isto é, a caracterização do uso da Internet por professores das disciplinas mencionadas. Os resultados mostram que os professores têm potencial expresso para utilização das redes eletrônicas mas precisam se dedicar mais e investir mais no desenvolvimento de metodologias e instrumentos para o ensino-aprendizagem a partir das redes eletrônicas. The main goal of the study was first to map, through the hipertext occurences of acess to web information from students of Biology and Physics of a special high school and second, caracterizar a atuação dos professores dessas mesmas áreas no uso da Internet. In this article, presents the results of this second part of the research. The results show that professors have a potential, expresso, to use of electronic net bu need more dedication and investment to develop metodologies and tools to the learning environment throught WWW.

  12. Identification of a Novel EF-Loop in the N-terminus of TRPM2 Channel Involved in Calcium Sensitivity

    Directory of Open Access Journals (Sweden)

    Yuhuan Luo

    2018-06-01

    Full Text Available As an oxidative stress sensor, transient receptor potential melastatin 2 (TRPM2 channel is involved in many physiological and pathological processes including warmth sensing, ischemia injury, inflammatory diseases and diabetes. Intracellular calcium is critical for TRPM2 channel activation and the IQ-like motif in the N-terminus has been shown to be important by mediating calmodulin binding. Sequence analysis predicted two potential EF-loops in the N-terminus of TRPM2. Site-directed mutagenesis combining with functional assay showed that substitution with alanine of several residues, most of which are conserved in the typical EF-loop, including D267, D278, D288, and E298 dramatically reduced TRPM2 channel currents. By further changing the charges or side chain length of these conserved residues, our results indicate that the negative charge of D267 and the side chain length of D278 are critical for calcium-induced TRPM2 channel activation. G272I mutation also dramatically reduced the channel currents, suggesting that this site is critical for calcium-induced TRPM2 channel activation. Furthermore, D267A mutant dramatically reduced the currents induced by calcium alone compared with that by ADPR, indicating that D267 residue in D267–D278 motif is the most important site for calcium sensitivity of TRPM2. In addition, inside-out recordings showed that mutations at D267, G272, D278, and E298 had no effect on single-channel conductance. Taken together, our data indicate that D267–D278 motif in the N-terminus as a novel EF-loop is critical for calcium-induced TRPM2 channel activation.

  13. Embryonal Fyn-associated substrate (EFS) and CASS4: The lesser-known CAS protein family members.

    Science.gov (United States)

    Deneka, Alexander; Korobeynikov, Vladislav; Golemis, Erica A

    2015-10-01

    The CAS (Crk-associated substrate) adaptor protein family consists of four members: CASS1/BCAR1/p130Cas, CASS2/NEDD9/HEF1/Cas-L, CASS3/EFS/Sin and CASS4/HEPL. While CAS proteins lack enzymatic activity, they contain specific recognition and binding sites for assembly of larger signaling complexes that are essential for cell proliferation, survival, migration, and other processes. All family members are intermediates in integrin-dependent signaling pathways mediated at focal adhesions, and associate with FAK and SRC family kinases to activate downstream effectors regulating the actin cytoskeleton. Most studies of CAS proteins to date have been focused on the first two members, BCAR1 and NEDD9, with altered expression of these proteins now appreciated as influencing disease development and prognosis for cancer and other serious pathological conditions. For these family members, additional mechanisms of action have been defined in receptor tyrosine kinase (RTK) signaling, estrogen receptor signaling or cell cycle progression, involving discrete partner proteins such as SHC, NSP proteins, or AURKA. By contrast, EFS and CASS4 have been less studied, although structure-function analyses indicate they conserve many elements with the better-known family members. Intriguingly, a number of recent studies have implicated these proteins in immune system function, and the pathogenesis of developmental disorders, autoimmune disorders including Crohn's disease, Alzheimer's disease, cancer and other diseases. In this review, we summarize the current understanding of EFS and CASS4 protein function in the context of the larger CAS family group. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. VizieR Online Data Catalog: UY UMa and EF Boo compiled time of minima (Yu+, 2017)

    Science.gov (United States)

    Yu, Y.-X.; Zhang, X.-D.; Hu, K.; Xiang, F.-Y.

    2017-11-01

    In order to construct the (O-C) diagram to analyze the period change of UY UMa, we have performed a careful search for all available times of light minima. A total of 76 times of light minima were collected and listed in Table 2. >From the literatures and two well-known databases (i.e., the O-C gateway (http://var.astro.cz/ocgate) and the Lichtenknecker database of the BAV (http://www.bav-astro.de/LkDB/index.php)), we have collected a total of 75 available times of light minima for EF Boo, which are summarized in Table 3. (3 data files).

  15. The Main Correlations of the Hungarian’s Health Status and Food Consumption

    Directory of Open Access Journals (Sweden)

    Bakos Izabella Mária

    2016-11-01

    Full Text Available It is a general socio-political objective of the mid- and long term food industry development strategy of Hungary to promote healthy food production and consumption. The realization of the strategy of the domestic food industry increasingly promotes healthy eating, for example consuming natural, domestic, fresh ingredients, prepared foods, in order to improve the overall health of the population (EFS, 2014-2020. Our study presents the regional tendencies of staple food consumption in Hungarian regions and the changes in indicators reflecting the health status of the population. Furthermore, our hypothesis states that there is a statistically provable correlation between the annual food consumption of Hungarian households per capita and the health status, on regional level.

  16. Ethical Food Consumption

    DEFF Research Database (Denmark)

    Heerwagen, Lennart Ravn

    So-called ‘ethical’ food products have spread across the industrialised world. These are products that are produced under labelling schemes with extraordinary attentiveness to issues such as farm animal welfare and environmental protection. Political decision-makers and other stakeholders in food...... protection. In particular, it aims to examine the concrete improvements that may be pursued through markets for ethical food, and how these improvements are influenced by factors related to individual consumers’ choice of food. This thesis is structured around three research papers that illuminate different...... aspects of ethical food consumption and, based on this, provide concrete policy inputs. The scope of the research is highly interdisciplinary, and includes perspectives from ethics and the social sciences on food consumption. Paper I: Can increased organic consumption mitigate climate changes...

  17. Seafood Consumption and Health

    Directory of Open Access Journals (Sweden)

    Hasan Huseyin Atar

    2009-04-01

    Full Text Available This study discussed relationship between healthy life and seafood consumption. Seafood consumption provide with predicting some chronic diseases, regular diet and a healthy eating habit. Fish and seafood have been promoted as healthy eating by health organizations in many countries all over the world. Seafood includes many important fatty acids, proteins, vitamins and minerals. These have been shown to cause significant biochemical and physiological changes in the body. Especially omega–3 and omega–6 fatty acids have been prevented some major diseases; such as CHD, cancer. [TAF Prev Med Bull 2009; 8(2.000: 173-176

  18. Why Taxing Consumption?

    DEFF Research Database (Denmark)

    Landes, Xavier

    2015-01-01

    Robert Frank is famous for proposing an incremental tax on consumption. His proposition is motivated by the control of positional externalities, i.e. the costs that individuals impose on each other when they consume goods for securing or acquiring social status. A close analysis of Frank...... are controversial while the invocation of efficiency is actually grounded in an underlying view of social cooperation. Secondly, this chapter advances the idea that an ultimate justification for the choice of specific tax base (consumption, income and wealth) expresses such an underlying view. In other words...

  19. The MazEF Toxin-Antitoxin System Alters the β-Lactam Susceptibility of Staphylococcus aureus.

    Directory of Open Access Journals (Sweden)

    Christopher F Schuster

    Full Text Available Toxin-antitoxin (TA systems are genetic elements of prokaryotes which encode a stable toxin and an unstable antitoxin that can counteract toxicity. TA systems residing on plasmids are often involved in episomal maintenance whereas those on chromosomes can have multiple functions. The opportunistic pathogen Staphylococcus aureus possesses at least four different families of TA systems but their physiological roles are elusive. The chromosomal mazEF system encodes the RNase toxin MazF and the antitoxin MazE. In the light of ambiguity regarding the cleavage activity, we here verify that MazF specifically targets UACAU sequences in S. aureus in vivo. In a native strain background and under non-stress conditions, cleavage was observed in the absence or presence of mazE. Transcripts of spa (staphylococcal protein A and rsbW (anti-σB factor were cut, but translational reporter fusions indicated that protein levels of the encoded products were unaffected. Despite a comparable growth rate as the wild-type, an S. aureus mazEF deletion mutant was more susceptible to β-lactam antibiotics, which suggests that further genes, putatively involved in the antibiotic stress response or cell wall synthesis or turnover, are controlled by this TA system.

  20. Load cell adoption in an electronic drag force flowmeter Medidor de vazão eletrônico com célula de carga

    Directory of Open Access Journals (Sweden)

    Antonio Pires de Camargo

    2011-06-01

    Full Text Available This research introduces the development of an electronic flowmeter based on the drag force that a body experiences when immersed in a fluid stream. Its main goal was the development of an Electronic Drag Force Flowmeter (EDFF using a load cell, as well as the evaluation of its performance parameters. The developed flowmeter should not require specialized labor, equipments, computers or any sophisticated and complex method, providing an easy and accurate way of flow estimation. This research was carried out in the following stages: (i EDFF mechanical structure development; (ii data acquisition system and embedded software design; and (iii evaluation of EDFF performance parameters. EDFF has routines for instantaneous flow rate measurement, interactive calibration, and also several flow meter parameter adjustments, allowing data transmission via a RS232 protocol. The real-time flow measurement task updates values of instantaneous flow rate each seven seconds, enabling unit selection. The interactive calibration routine guides users during all calibration process showing instructions on EDFF's display. A data digital filtering procedure was implemented in an embedded software using the Grubbs' Test in order to identify and to remove outliers from the acquired data. The Method of Least Squares was also implemented in the embedded software in order to calculate the fitting model coefficients on the calibration procedure. This flowmeter is able to work from 1.94 to 7.78 dm³ s-1 with an uncertainty of ± 5.7%. The coefficient of local head loss (K was close to 0.55 for Reynolds number values higher than 10(5. The developed EDFF is a low-cost and stand-alone system with potential for agricultural applications.Este estudo apresenta o desenvolvimento de um medidor de vazão baseado na força de arraste que atua em um corpo imerso em uma corrente líquida. O principal objetivo foi o desenvolvimento de um Medidor de Vazão Eletrônico tipo Força (MVEF

  1. Preclinical validation of the hypoxia tracer 2-(2-nitroimidazol-1-yl)-N-(3,3,3-[18F]trifluoropropyl)acetamide, [18F]EF3

    International Nuclear Information System (INIS)

    Mahy, P.; De Bast, M.; Gregoire, V.; Leveque, P.H.; Gillart, J.; Labar, D.; Marchand, J.

    2004-01-01

    The 2-nitroimidazole derivative 2-(2-nitroimidazol-1-yl)-N-(3,3,3-trifluoropropyl)acetamide (EF3) is a marker which forms adducts into hypoxic cells. Radiosynthesis of [ 18 F]EF3 was recently performed by our group. Our aim was to study the pharmacokinetics, biodistribution, metabolism and specificity for hypoxia of [ 18 F]EF3. MCa-4, SCC VII, NFSA, FSA, FSA II or Sa-NH tumour-bearing C3H mice were injected intravenously with [ 18 F]EF3 and allowed to breathe air, 10% O 2 or carbogen until sacrifice 5-770 min after injection. Radioactivity was measured ex vivo in various organs, including urine and faeces. Selected organs were additionally processed to measure tracer metabolites with high-performance liquid chromatography. The half-life in blood was 73.9 min. [ 18 F]EF3 was eliminated mainly via the kidneys, with 75% of the injected activity found in the urine by 12 h 50 min. The biodistribution was fast and homogeneous except in the brain and the bone, where it was significantly lower, and in the liver and the kidney, where it was significantly higher. In most organs, the exceptions being the gastrointestinal and urinary tract, tissue-to-blood ratios were below or close to unity. In tumours, a relative accumulation of the tracer was observed with time, which, at 220 min after injection, depended on tumour strain and oxygenation conditions, i.e. 10% O 2 significantly increased the tumour-to-muscle ratio whereas carbogen decreased it. [ 18 F]EF3 was rapidly metabolised in the kidney and the liver. [ 18 F]EF3 is a promising tracer for detection of tumour hypoxia. A phase I study in head and neck cancer patients is in progress at our institution. (orig.)

  2. Isoforms of elongation factor eEF1A may be differently regulated at post-transcriptional level in breast cancer progression

    Directory of Open Access Journals (Sweden)

    Vislovukh A. A.

    2013-01-01

    Full Text Available Eukaryotic translation elongation factor 1A exists as two 98 % homologous isoforms: eEF1A1 (A1 and eEF1A2 (A2 which are tissue and development specific. Despite high homology in an open reading frame (ORF region, mRNAs coding for eEF1A1 and eEF1A2 are different in their untranslated regions (UTR, suggesting a possibility of their dissimilar post-transcriptional regulation. Aim. To analyze the existence of cis-acting motifs in the UTRs of EEF1A1/A2 mRNAs, to confirm the possibility of post-transcriptional control of eEF1A1 and eEF1A2 expression. Methods. An ensemble of bioinformatic methods was applied to predict regulatory motifs in the UTRs of EEF1A1/A2 mRNAs. Dual-luciferase reporter assay was employed to detect post-transcriptional regulation of eEF1A1/A2 expression. Results. Numerous regulatory motifs in the UTR of EEF1A1/A2 mRNAs were found bioinformatically. The experimental evidence was obtained for the existence of negative regulation of EEF1A1 and positive regulation of EEF1A2 mRNA in the model of breast cancer development. Conclusions. EEF1A1 and EEF1A2 mRNAs contain distinct motifs in the UTRs and are differently regulated in cancer suggesting the possibility of their control by different cellular signals.

  3. Turkey's net energy consumption

    International Nuclear Information System (INIS)

    Soezen, Adnan; Arcaklioglu, Erol; Oezkaymak, Mehmet

    2005-01-01

    The main goal of this study is to develop the equations for forecasting net energy consumption (NEC) using an artificial neural-network (ANN) technique in order to determine the future level of energy consumption in Turkey. In this study, two different models were used in order to train the neural network. In one of them, population, gross generation, installed capacity and years are used in the input layer of the network (Model 1). Other energy sources are used in input layer of network (Model 2). The net energy consumption is in the output layer for two models. Data from 1975 to 2003 are used for the training. Three years (1981, 1994 and 2003) are used only as test data to confirm this method. The statistical coefficients of multiple determinations (R 2 -value) for training data are equal to 0.99944 and 0.99913 for Models 1 and 2, respectively. Similarly, R 2 values for testing data are equal to 0.997386 and 0.999558 for Models 1 and 2, respectively. According to the results, the net energy consumption using the ANN technique has been predicted with acceptable accuracy. Apart from reducing the whole time required, with the ANN approach, it is possible to find solutions that make energy applications more viable and thus more attractive to potential users. It is also expected that this study will be helpful in developing highly applicable energy policies

  4. Exploring heroin consumption

    NARCIS (Netherlands)

    Trautmann, Franz; Frijns, Tom

    2013-01-01

    In this report we explore some aspects of heroin consumption, using the data we collected through the face-to-face interviews and comparing our findings with data from other research and monitoring sources. We focus on Italy, the Netherlands and England, the three sample Member States where we have

  5. Regional final energy consumptions

    International Nuclear Information System (INIS)

    2011-01-01

    This report comments the differences observed between the French regions and also between these regions and national data in terms of final energy consumption per inhabitant, per GDP unit, and per sector (housing and office building, transport, industry, agriculture). It also comments the evolutions during the last decades, identifies the most recent trends

  6. Bus fuel consumption model

    Energy Technology Data Exchange (ETDEWEB)

    Zargari, S.A. [Iran Univ. of Science and Technology, Teheran (Iran, Islamic Republic of); Khan, A.M. [Carleton Univ., Ottawa, ON (Canada). Dept. of Civil and Environmental Engineering

    2000-07-01

    The interest in rapid bus transit has increased sharply with the realization that modern metropolitan areas rely on public transit to provide for strong economies and communities. As a prevention tool against traffic congestion, deteriorating air quality and rising greenhouse gas emissions, this study of bus fuel consumption was designed to assist in the planning and management of rapid bus transit. The Australian Road Research Board's (ARRB) Road Fuel Consumption Model was used as a starting point. The estimations required were realized with the help of Newtonian Mechanics. The four states of vehicular traffic were examined: acceleration, cruise, deceleration, and idle. The estimated total power required from the engine to overcome resistance forces, to run vehicle accessories and overcome internal engine friction was calculated. The data for the standard and articulated bus was obtained from OC Transpo in Ottawa. The study permitted the authors to conclude that the estimations for the parameters for power requirements and fuel consumption for heavy duty vehicles are appropriate. The methodology for the estimation of fuel consumption on the Transitway, which is part of the rapid bus transit system, proved adequate. In addition, the methodology was useful to estimate fuel savings resulting from demand management strategies with potential for modal shift. 9 refs., 6 tabs.

  7. Sustainable consumption and marketing

    NARCIS (Netherlands)

    Dam, van Y.K.

    2016-01-01

    Sustainable development in global food markets is hindered by the discrepancy between positive consumer attitudes towards sustainable development or sustainability and the lack of corresponding sustainable consumption by a majority of consumers. Apparently for many (light user) consumers the

  8. Consumption growth accounting

    NARCIS (Netherlands)

    Dietzenbacher, Erik; de Groot, Olaf J.; Los, Bart

    The methodology in this paper combines an input-output structural decomposition approach with the supply-side perspective of mainstream growth accounting. In explaining the intertemporal change in consumption per worker, three sets of effects are distinguished. First, contributions due to several

  9. Overview of Alcohol Consumption

    Science.gov (United States)

    ... of Alcohol Consumption Alcohol's Effects on the Body Alcohol Use Disorder Fetal Alcohol Exposure Support & Treatment Alcohol Policy Special ... experience alcohol’s longer-term effects, which can include: Alcohol use disorder Health problems Increased risk for certain cancers In ...

  10. Organic consumption behavior

    NARCIS (Netherlands)

    Du, Shuili; Bartels, Jos; Reinders, Machiel; Sen, Sankar

    2017-01-01

    Consumer demand for organic food and non-food products has been growing dramatically. This study examines organic consumption behavior from a social identification perspective. Focusing on the central role of organic consumer identification (OCI), or the extent to which individuals categorize

  11. The Ideology of Consumption

    DEFF Research Database (Denmark)

    Hansen, Brian Benjamin

    2013-01-01

    This article opts for a return to a critique of the ideology of consumption. Following Slavoj Žižek it argues that what must be addressed in present-day consumer-capitalism is the level of the superego. Superego is not about living up to certain norms/standards; rather, superego fits consumerism...

  12. The Consumption Paradigm in Marketing

    Directory of Open Access Journals (Sweden)

    Eka Ardianto

    2003-08-01

    Full Text Available This article elaborates consumption paradigm in marketing. In background, this paper reviews different perspectives of consumption: economic perspective and marketing perspective. In ontology, this work describes various issues regarding consumption view. In epistemology, this article demonstrates how marketers especially researches explore the consumption phenomena. In methodology, the article describes experiential marketing –one of applied consumption paradigm in marketing, which could be an alternative choice of marketing practices.

  13. Country differences in sustainable consumption

    DEFF Research Database (Denmark)

    Thøgersen, John

    2010-01-01

    In a sustainability perspective, consumption research has an unfortunate individualizing bias, which means that macro and structural causes of unsustainable consumption tend to be ignored. Hence, a comprehensive model of determinants of the sustainability of consumption is developed and applied...... on a specific case: organic food consumption. The analyzed data are published research on why consumer purchase of organic food products differs between countries. As expected, organic food's share of total food consumption depends heavily on political regulation, including legal definitions and standards...

  14. Work-related consumption drivers and consumption at work

    DEFF Research Database (Denmark)

    Røpke, Inge

    2004-01-01

    The main message in this paper is that the discussion on sustainable consumption should also incorporate the consumption that occurs in relation to work and, more generally, the relationship between consumption at work and consumption at home. I start by considering how domestic consumption...... is encouraged by work-related factors and continue to consider how consumption activities occur in the workplace, so illustrating that production and consumption are intertwined. The main part of the paper deals in detail with the conceptual distinction between production and consumption. Inspiration is drawn...... from both ecology and economics with focus on some important predecessors for ecological economics. I conclude with reflections on how to proceed with consumption studies to provide the basis for promoting more sustainable life patterns....

  15. Influências do Comércio Eletrônico B2B no Processo de Atendimento de Pós-Vendas no Segmento de Bens de Capital: Estudo de Caso em uma Empresa Representante de Equipamentos Pesados

    Directory of Open Access Journals (Sweden)

    Tiago Correa do Amaral

    2015-06-01

    Full Text Available Estratégias competitivas são identificadas como norteadoras e pontos divergentes entre as empresas, que por sua vez garantem a evolução do mercado. Mais especificamente no setor de bens de capital, a necessidade de se diferenciar em suporte se traduz na exigência em disponibilidade de máquina, pois somente assim é possível proporcionar o retorno sobre o investimento realizado na aquisição do equipamento. O atendimento em pós-vendas, que visa suportar o produto em condições operacionais, é um relacionamento de longo prazo e que deve ser realizado com excelência com o intuito de proporcionar segurança e confiança e, consequentemente, fidelizar o cliente. A agilidade desse atendimento e o provisionamento de soluções rápidas é essencial para o consumidor e, por isso, a hipótese que o comércio eletrônico pode agregar valor nesse processo foi testada em uma empresa representante de equipamentos pesados de mais de 70 anos de mercado. A pesquisa foi conduzida através de entrevistas exploratórias com profissionais que atuam diretamente no relacionamento com clientes do segmento de equipamentos pesados para construção. Portanto, o objetivo desse trabalho foi identificar como o comércio eletrônico pode influenciar na qualidade de atendimento, velocidade de resposta e redução de custos no processo de atendimento em pós-vendas nas relações B2B. Os resultados apontam que a efetiva implantação de uma ferramenta de tecnologia da informação, como o comércio eletrônico, pode proporcionar vantagens competitivas através de um atendimento assertivo e otimizado em pós vendas.

  16. Coordenação das atividades produtivas na indústria brasileira de jogos eletrônicos: hierarquia, mercado ou aliança? Coordination of production activities in the Brazilian electronic games industry: hierarchy, market or alliance?

    Directory of Open Access Journals (Sweden)

    Alexandre Perucia

    2011-03-01

    Full Text Available Este estudo busca compreender como as empresas que desenvolvem jogos eletrônicos coordenam suas atividades de produção na indústria brasileira de jogos eletrônicos. Têm-se como base teórica os custos de transação e as relações interorganizacionais para a análise das estratégias de produção interna (hierarquia, contratação no mercado (mercado e colaboração (aliança. A pesquisa foi conduzida junto às empresas da Associação Brasileira de Desenvolvedoras de Games (ABRAGAMES, e os dados foram coletados por meio de questionários eletrônicos e entrevistas em profundidade. Os resultados indicam a predominância da internalização das atividades de produção dos jogos, em função da existência de custos transacionais associados à necessidade de ativos específicos como especialização do conhecimento e escassez da mão de obra. Entretanto, observaram-se casos de estratégias de subcontratação alinhadas a atividades menos estratégicas para as empresas e de projetos colaborativos que potencializaram ganhos como redução de incerteza, flexibilidade organizacional, melhoria de processos e aprendizagem.This study aims to understand how games firms coordinate their production activities in the Brazilian Electronic Games Industry. To do so this paper makes use of the theories of transaction costs as well as of inter-organizational relationships to analyze firms' strategic choices with regard to games production: made in-house (Hierarchy, bought on the market (Market, or collaboration with a partner (Alliance. The research was conducted with firms belonging to the Brazilian Game Developers Association and data were collected from electronic surveys and interviews. The results show that firms tend to internalize activities of game production, in particular because of transaction costs associated with asset specificity (knowledge specialization and scarce availability of skilled labor. However, market relations were found

  17. Evaluation of {sup 18}F-BCPP-EF for mitochondrial complex 1 imaging in the brain of conscious monkeys using PET

    Energy Technology Data Exchange (ETDEWEB)

    Tsukada, Hideo; Ohba, Hiroyuki; Kanazawa, Masakatsu; Kakiuchi, Takeharu; Harada, Norihiro [Hamamatsu Photonics K.K., Central Research Laboratory, Hamamatsu, Shizuoka (Japan)

    2014-04-15

    We have reported on the development of a novel PET probe, {sup 18}F-2-tert-butyl-4-chloro-5-{6-[2-(2-fluoroethoxy)-ethoxy] -pyridin-3-ylmethoxy}-2H-pyridazin-3-one ({sup 18}F-BCPP-EF), for quantitative imaging of mitochondrial complex 1 (MC-1) activity in the brain of the living rat. For clinical application in humans, translational research in the monkey was conducted. PET measurements with {sup 18}F-BCPP-EF were performed in young and old monkeys (Macaca mulatta) in a conscious state with arterial blood sampling. The binding specificity of {sup 18}F-BCPP-EF was evaluated with rotenone, a specific MC-1 inhibitor, in young animals. The binding (total distribution volume, V{sub T}) of {sup 18}F-BCPP-EF was calculated using Logan graphical analysis, and one-tissue compartment model (1-TC) and two-tissue compartment model (2-TC) analyses using a metabolite-corrected plasma input function. F-BCPP-EF was rapidly taken up into the brain just after intravenous injection, peaked between 10 and 20 min after injection, and was then gradually eliminated. The 2-TC analysis provided a better fit than the 1-TC analysis, and the V{sub T} values from the 2-TC analysis correlated well with those from the Logan plot. With predosing with rotenone, {sup 18}F-BCPP-EF showed a higher uptake peak in the brain, followed by more rapid elimination thereafter than in the vehicle condition, resulting in significant reductions in 2-TC V{sub T} values in all regions. In old animals, the kinetics of {sup 18}F-BCPP-EF were slightly slower with lower peak levels than in young animals, resulting age-related reductions in {sup 18}F-BCPP-EF binding in all brain regions. The present study demonstrated that {sup 18}F-BCPP-EF may be a potential PET probe for quantitative imaging MC-1 activity in the living brain using PET. (orig.)

  18. Characterization of novel peptide-specific antibodies against the translation elongation factor eEF1A2 and their application for cancer research

    Directory of Open Access Journals (Sweden)

    Shalak V. F.

    2014-11-01

    Full Text Available Aim. We intend to characterize the new peptide-specific antibodies against the isoform 2 of translation elongation factor 1A (eEF1A2 and determine its presence in the postoperative samples of human breast, lung and stomach tumor tissues. Methods. The analysis of antibody specificity was performed by enzyme-linked immunosorbent assay, immunoblotting and immunohistochemistry. Immunoblotting and immunohistochemistry were used for the determination of the eEF1A2 in the human tumor samples, as well as in the samples of normal tissues surrounding tumors. Results. The antibodies obtained against the eEF1A2 specifically recognized this protein in the cell extracts and histological sections and did not cross-react with the elongation factor 1A isoform 1. eEF1A2 was revealed in the postoperative samples of breast, lung and stomach tumors as well as in the putative normal tissues surrounding tumors. Conclusions. The antibodies obtained against eEF1A2 are highly specific for the antigen and can be used for the immunological studies of tumors.

  19. Fuelwood consumption in Newfoundland

    Energy Technology Data Exchange (ETDEWEB)

    1980-01-01

    This study was undertaken to get a first estimate of fuelwood consumption for the island of Newfoundland for the period April 1, 1977 to March 31, 1978. Available literature was reviewed and an assessment made on the existing data collection systems on fuelwood consumption used by the various forestry agencies in the province. A personal interview telephone survey on fuelwood use was conducted for most parts of the Island that had access to wood supplies. Householders were asked how much wood they burned, when they started burning wood, type of wood burning unit used, other sources of heat used, reasons for burning wood, employment, distance travelled to obtain wood, and expected consumption in 1978-79 compared to 1977-78. It was found that the estimated number of households burning wood increased to ca 32,000 in 1977-78. There was a further estimated increase to about 39,500 in 1978-79. In 1977-78, it was estimated that 461,571 stacked cubic metres of fuelwood were consumed on the Island. This was broken down into 318,916 m/sup 3/ of softwood and 142,655 m/sup 3/ of hardwood. Although the statistical methodology used may have led to an overestimate of the volume of wood consumed, the criteria used in the selection of households had the opposite effect in that the volume consumed by a large number of casual wood burners (less than 3 stacked m/sup 3/) was omitted from the estimate. Fuelwood consumption accounted for anywhere from 1% to 77% of the calculated allowable annual cut for the various forest management units on the Island for 1977-78. Overall, fuelwood consumption estimated by the survey accounted for 8% of the estimated allowable annual cut. Use of wood as a fuel is expected to continue increasing and it is recommended that estimates of consumption be periodically updated so that forest management plans can make adequate provision for this demand in future. 5 refs., 16 figs., 7 tabs.

  20. Compras na Administração Pública: o Pregão Eletrônico como Instrumento de Eficiência diante das Modalidades da Lei nº 8.666/93

    OpenAIRE

    André Lezan Fernandes; Antônio Gonçalves de Oliveira

    2015-01-01

    O bom uso dos recursos públicos, a transparência dos processos de compras governamentais e o uso de novas tecnologias são elementos fundamentais para o bom funcionamento da máquina pública contemporânea. Este estudo tem por objetivo discorrer sobre a modalidade pregão eletrônico de licitações públicas. O método utilizado para a elaboração deste estudo foi baseado no estudo bibliográfico de obras existentes que tratam sobre o tema. A modalidade pregão surgiu no início dos anos 2000 como uma no...

  1. Avaliação de acessibilidade e informações sobre políticas públicas para pessoas com deficiência em portais eletrônicos de governo paulistas

    OpenAIRE

    Didoni, Bianca [UNESP

    2013-01-01

    O advento das tecnologias traz para a área da comunicação pública a possibilidade de mudar o processo de diálogo entre governo e cidadão. Isto implica o fortalecimento da democracia, tornando a sociedade mais participativa com acesso a informações. Esta pesquisa avaliou a acessibilidade e a presença de informações sobre políticas públicas para as pessoas com deficiência disponíveis nos portais eletrônicos das 15 cidades sede das regiões administrativas do Estado de São Paulo: Araçatuba, Arara...

  2. Gestão da qualidade da informação no contexto das organizações: percepções a partir do experimento de análise da confiabilidade dos jornais eletrônicos

    Directory of Open Access Journals (Sweden)

    José Osvaldo De Sordi

    Full Text Available A presente pesquisa aborda a complexidade envolta na definição de atributos que permitam mensurar dimensões da qualidade da informação. Para isso, discute-se um experimento prático, que analisa em profundidade uma das dimensões da qualidade da informação: a confiabilidade. A análise realizada abrange a versão eletrônica de cada um dos dez maiores jornais brasileiros impressos em circulação média diária. A experiência constatou ser crítico ao processo de análise da qualidade da informação o pleno discernimento dos atributos importantes de serem analisados, além dos aspectos importantes de serem observados para cada um dos atributos.

  3. Metodologia para observação da camada de cera em maçãs, utilizando microscopia eletrônica de varredura Methodology for observation of the wax layer in apples, using scanning electron microscopy

    Directory of Open Access Journals (Sweden)

    Luis Antônio Suita de Castro

    2002-12-01

    Full Text Available A estrutura mais superficial da epiderme dos frutos é constituída por fina camada de cera, responsável por mecanismos de proteção, principalmente relacionados à evapotranspiração e ao ataque de patógenos. A visualização de cera por microscopia eletrônica de varredura apresenta dificuldades no processamento da amostra, pois os métodos rotineiramente utilizados ocasionam sérios danos a esta camada ou, simplesmente, provocam sua eliminação devido ao processo de solubilização causado pelos reagentes utilizados. Alguns autores têm utilizado temperaturas baixas (-90ºC para o processamento de amostras. No Brasil, tentativas de observação de cera em maçãs, utilizando a microscopia eletrônica de varredura, têm sido realizadas; entretanto, os resultados não foram satisfatórios. No Laboratório de Microscopia Eletrônica da Embrapa Clima Temperado, foi adaptado um método simples e que tem apresentado bons resultados. Para este experimento, foram retiradas pequenas porções da casca de maçãs da região equatorial dos frutos. As amostras foram fixadas em lâminas histológicas, utilizando fita adesiva nas extremidades. Posteriormente, foram colocadas em dessecador contendo sílica gel, para que ocorresse a desidratação. Após 72 horas, foram retirados fragmentos para fixação em stubs e metalização com ouro. Foi usado um microscópio eletrônico ZEISS (DSM-940A, regulado à distância de trabalho de 15mm, voltagem de aceleração de 10 kV e ampliação de 3.000 X. As imagens obtidas permitiram avaliar a interferência dos fatores ambientais utilizados no processo de frigoconservação de maçãs.The fruit epidermis is covered by a thin layer of wax which gives protection to the fruit, mainly against water loss and pathogenic organisms. Several studies using scanning electron microscope have been developed to study that wax. The wax layer visualization through the scanning microscope is difficult due to some problems of

  4. Determinação da energia descartada e da emissão de CO2 ao longo do ciclo de vida de produtos eletrônicos tendo em vista a obsolescência programada

    OpenAIRE

    Pereira, Swilann Mendes

    2016-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Mecânica, Florianópolis, 2016. Os impactos ambientais e sociais associados ao rápido crescimento de resíduos de equipamentos elétricos e eletrônicos têm sido globalmente reconhecidos como um risco emergente para as organizações, devido aos crescentes volumes gerados e às substâncias tóxicas presentes em sua composição. Por outro lado, o processo de fabricação desses ...

  5. Videogames, consumption and education

    Directory of Open Access Journals (Sweden)

    Felix ETXEBERRIA VALERDI

    2017-07-01

    Full Text Available The ever-increasing success and use of videogames is a reality in our current society, being 54% of the total consumption of audiovisual or interactive entertainment in Spain in 2007. One of the aspects to be taken into consideration, with respect to the education of children, is the implication for consumption in relation to videogames. The violence portrayed in these games is one of the main topics of debate in the literature about and studies on the effects of videogames on children’s behaviour. However, it is not just a question of protecting minors against the risks of videogames use without control and care exercised by parents or educators. For many years now, despite the risks involved in their use, videogames have been used as another medium in education itself.

  6. Consumption and Needs

    DEFF Research Database (Denmark)

    Læssøe, Jeppe

    1996-01-01

    The contribution takes its point of departure in the relation between the many resource savings initiatives and the lack of interest for the growth in consumption. It argues that what we make up on the roundabouts, we risk to loose on the swings. In order to prevent that it reveals some of the cu...... socio-cultural and life qualitative demands into account. As a conclusion the author outlines some ideals with regard to how to integrate human the dimension in urban ecological design.......The contribution takes its point of departure in the relation between the many resource savings initiatives and the lack of interest for the growth in consumption. It argues that what we make up on the roundabouts, we risk to loose on the swings. In order to prevent that it reveals some...

  7. Sustainable Food Consumption

    DEFF Research Database (Denmark)

    Reisch, Lucia; Eberle, Ulrike; Lorek, Sylvia

    2013-01-01

    Contemporary food production and consumption cannot be regarded as sustainable and raises problems with its wide scope involving diverse actors. Moreover, in the face of demographic change and a growing global population, sus-tainability problems arising from food systems will likely become more...... and globalization of agriculture and food processing, the shift of consumption patterns toward more dietary animal protein, the emergence of modern food styles that entail heavily processed products, the growing gap on a global scale between rich and poor, and the paradoxical lack of food security amid an abundance...... of food. These factors are attributable to national and international policies and regulations, as well as to prevalent business prac-tices and, in particular, consumers' values and habits. The most effective ways for affluent societies to reduce the environmental impact of their diets are to reduce...

  8. Youth and Tourism Consumption

    Directory of Open Access Journals (Sweden)

    Abdolhossein Kalantari

    2008-07-01

    Full Text Available This paper tends to study tourism attitudes among the youth. It argues that in studying tourism among the youth, it is necessary to consider youth’s other behavioral factors in addition to the youth subculture. Therefore, we should study the youth culture from the view point of “Consumption”. In this view, youth tourism is equal to consumption of time, space and signs. Using ongoing theoretical debates and division, we would attempt to explore various factors of youth tourism. This article shows that youth tourism and youth culture are so mutually interconnected that we should comprehend youth tourism based on youth culture and vise versa. In conclusion, analyzing the youth subculture which is rooted in their consumption attitudes, the study attempts to understand youth tourism.

  9. Promoting seafood consumption

    DEFF Research Database (Denmark)

    Scholderer, Joachim; Grunert, Klaus G.

    was that preparing a tasty meal from fresh fish was actually not difficult. In supplemetary materials consumers were provided with recipes for fresh fish. At the same time, major retail chains made MAP-packaged fresh fish filets available in Danish supermarkets. The influence of familiy expectations was harnessed......Before and after the 3-year generic advertising campaign for fresh fish in Denmark, representative consumer samples were surveyed with regard to the attitudes towards fresh fish, perceived family norms, availability of fresh fish in shops, meal preparation skills, intentions to buy fresh fish......, and actual consumption frequencies. In the pre-campaign survey (effective N=641), significant determinants of consumption were availability in shops, meal preparation skills, and intentions to buy fresh fish. The campaign was especially designed to conquer these barriers. The key proposition...

  10. Fuelwood consumption in Newfoundland

    Energy Technology Data Exchange (ETDEWEB)

    1979-01-01

    Fuelwood consumption increased in Newfoundland in the 1970's, but although cutting permits were issued for Crown land, most forests were privately leased and no overall figures were available on the annual cut for domestic use. A personal interview telephone survey was therefore implemented in which householders were questioned on how much wood they burned, when they started burning wood, type of stove and wood used, expected consumption in 1978-79, other sources of heat, employment, and distance travelled to obtain wood. Survey methods and results obtained are presented. Woodburning households increased from about 22,000 before 1973 to about 32,000 in 1977-78 and a further increase to about 39,500 was expected in 1978-79. In 1977-78, 462,571 stacked cubic metres of roundwood were burned on the island. Softwood comprised 69% of this total.

  11. The mediatization of ethical consumption

    DEFF Research Database (Denmark)

    Eskjær, Mikkel Fugl

    2013-01-01

    Over the years, mediatization studies have investigated the influence of media in numerous sections of contemporary society. One area that has received limited attention is the mediatization of consumption, particularly issues concerning ethical consumption. This article presents a study of how...... mediatization is transforming modern consumption and contributing to the mainstreaming of ethical consumption. Based on a study of a Danish online eco-store, the article argues that modern ethical consumption increasingly depends on new media practices to present sustainable consumption as practical...

  12. Sociology of Drug Consumption

    OpenAIRE

    2004-01-01

    In this article which is a review of sociological ideas and studies of drug abusers in social situation, drug addiction steps (particularly alcohol, heroin and cocaine consumption) are revised and some explanations are made. Also, the role of some sociological ideas in drug addiction is considered in which Anomie Theory reads: "because of such duality, the individuals who are not satisfied with their role are in hurt." According to this theory, drug users choose seclusion and neglecting usual...

  13. EF-hands at atomic resolution: The structure of human psoriasin (S100A7) solved by MAD phasing

    DEFF Research Database (Denmark)

    Brodersen, Ditlev Egeskov; Etzerodt, Michael; Madsen, Peder Søndergaard

    1998-01-01

    psoriasin reveals that this protein, in contrast to other S100 proteins with known structure, is not likely to strongly bind more than one calcium ion per monomer. The present study contradicts the idea that calcium binding induces large changes in conformation, as suggested by previously determined......The S100 family consists of small acidic proteins, belonging to the EF-hand class of calcium-binding proteins. They are primarily regulatory proteins, involved in cell growth, cell structure regulation and signal transduction. Psoriasin (S100A7) is an 11.7 kDa protein that is highly upregulated...... in the epidermis of patients suffering from the chronic skin disease psoriasis. Although its exact function is not known, psoriasin is believed to participate in the biochemical response which follows transient changes in the cellular Ca2+ concentration. RESULTS: The three-dimensional structure of holmium...

  14. Governo eletrônico – E-GOV: análise dos serviços disponíveis no portal do Governo do Estado da Bahia

    Directory of Open Access Journals (Sweden)

    Juliana Fachin

    2015-04-01

    Full Text Available O presente estudo exploratório analisou o Portal do Governo do estado da Bahia, em que categoriza,em nível de qualidade, os serviços do governo dispostos em meio eletrônico. Para a fundamentaçãoteórica, foi abordado o Acoplamento Tecnológico relacionado às mudanças estruturais na sociedade,o uso das Tecnologias de Informação e Comunicação (TICs, pelo Governo Eletrônico (E-GOV, pararealizar prestação de serviços via web. A análise do portal indicou que 60% dos serviços encontramseno nível 2, representando um sistema informacional de qualidade; com 34% ficaram o nível 3 e 4,com aplicações de serviços melhorados e transacionais. Quanto à averiguação do uso de ferramentasde acessibilidade nas páginas do portal do governo, pouco positivas foram as respostas, de sessentapáginas apenas sete contêm as ferramentas. Três as contém em subpáginas, uma não dispõe, masexplica como utilizar os atalhos do computador para ativar essas ferramentas, e 49 do total nãodispõem de nenhum tipo de ferramenta de acessibilidade. A pesquisa apontou para a ausência douso de ferramentas de acessibilidade e também reflete na aplicação de serviços em nível avançado,interativos e integrados como forma de prestação de serviço de qualidade.

  15. Estudo de usuários de web e-mail: a busca pela informação através dos portais eletrônicos

    Directory of Open Access Journals (Sweden)

    Gabrielle Francinne de Souza Carvalho

    2009-04-01

    Full Text Available Este trabalho tem como objetivo analisar dois tipos de abordagem – tradicional e alternativa – utilizadas nos estudos de usuário. Para isso, adotou-se os instrumentos de coleta de dados adequados a cada tipo de abordagem: o questionário, na abordagem tradicional, e a entrevista, na abordagem alternativa. Percebeu-se que o primeiro não permite estabelecer um diálogo mais estreito com o usuário, devido à limitação das respostas, uma vez que já estavam definidas. Já a entrevista possibilitou conhecer com mais detalhes as necessidades informacionais de cada usuário e como se efetiva seu processo de busca e uso. Assim, verificou-se através de quais portais eletrônicos os usuários buscam essa informação, procurando entender se esses portais são os mesmos que possuem vinculados os provedores de seus e-mails, visto que esses portais eletrônicos deveriam possibilitar também o suprimento de suas necessidades informacionais. Concluiu-se que a maioria dos sujeitos desta pesquisa não utiliza, por diversos motivos, seus provedores de e-mail principal para buscar informação. A partir dos resultados, observou-se que a primeira abordagem não reconhece as necessidades individuais de cada usuário, limitando-se à objetividade de um tratamento estatístico. Em contrapartida, a segunda abordagem reconhece a subjetividade e as necessidades desses usuários.

  16. A Constituição da Memória dos Procedimentos em Saúde no Contexto do Prontuário Eletrônico do Paciente.

    Directory of Open Access Journals (Sweden)

    Rosane Suely Alvares Lunardelli

    2014-02-01

    Full Text Available Introdução: Estudos realizados por diversas áreas do conhecimento em torno da memória evidenciam a importância de que se reveste esse tema para a sociedade contemporânea, extremamente fértil no que diz respeito à produção de novos conhecimentos e possíveis esquecimentos também.Objetivo: Nesse contexto, buscou-se caracterizar o prontuário eletrônico do paciente como valioso documento de constituição da memória da saúde da população.Metodologia: Apoiados em fundamentos teóricos relativos aos processos e tipos de memória foram apresentados alguns dos atributos do prontuário eletrônico do paciente, suas funções, finalidades e seu papel como um dos lugares de memória.Resultados: Inserido em categorias de memória no que diz respeito à sua abrangência, tempo de duração, mecanismos de criação e conteúdo, defende-se que o prontuário deva ser considerado, na contemporaneidade, como agente de constituição e preservação da memória procedimental.Conclusões: De acordo com o panorama apresentado, espera-se que as questões abordadas suscitem reflexões e promovam outros estudos a respeito desse documento aqui instituído como um dos mais significativos lugares de memória, de estabelecimento e preservação das ações, dos procedimentos realizados no âmbito dos cuidados em saúde. 

  17. Análise do sistema eletrônico de compras do governo federal brasileiro sob a perspectiva da criação de valor público

    Directory of Open Access Journals (Sweden)

    Ronaldo Inamine

    2012-03-01

    Full Text Available O sistema de compras públicas do Governo Federal tem como um de seus objetivos a busca da eficiência. Esse conceito, contudo, amplia-se na medida em que são discutidos na Administração Pública aspectos complexos da criação de valor público, como expressão mais próxima do interesse do cidadão. Buscou-se na literatura identificar categorias que expressam o valor público para, então, analisar o sistema eletrônico de compras do Governo Federal brasileiro. As categorias encontradas foram: eficiência, transparência, accountability e interesse público. Na estratégia de pesquisa adotada, foram empregados os conceitos do pensamento sistêmico-complexo na elaboração de um instrumento de diagnóstico que permite a consideração de inter-relações entre categorias de análise, já aplicado no Ministério do Planejamento, Orçamento e Gestão (MPOG, órgão responsável pela gestão do sistema eletrônico de compras no Governo Federal brasileiro. Os resultados demonstraram que a categoria eficiência destaca-se das demais. Contudo, existem perspectivas para o desenvolvimento das demais categorias. O uso do instrumento de diagnóstico permitiu a percepção de novas possibilidades que podem contribuir para o sistema alcançar objetivos mais amplos no âmbito das compras do Governo Federal, dentre eles a criação de valor público.

  18. Proteomic analysis of trichloroethylene-induced alterations in expression, distribution, and interactions of SET/TAF-Iα and two SET/TAF-Iα-binding proteins, eEF1A1 and eEF1A2, in hepatic L-02 cells

    Energy Technology Data Exchange (ETDEWEB)

    Hong, Wen-Xu; Yang, Liang; Chen, Moutong; Yang, Xifei; Ren, Xiaohu; Fang, Shisong; Ye, Jinbo; Huang, Haiyan; Peng, Chaoqiong; Zhou, Li; Huang, Xinfeng; Yang, Fan; Wu, Desheng; Zhuang, Zhixiong; Liu, Jianjun, E-mail: bio-research@hotmail.com

    2012-09-01

    Emerging evidence indicates that trichloroethylene (TCE) exposure causes severe hepatotoxicity. However, the mechanisms of TCE hepatotoxicity remain unclear. Recently, we reported that TCE exposure up-regulated the expression of the oncoprotein SET/TAF-Iα and SET knockdown attenuated TCE-induced cytotoxicity in hepatic L-02 cells. To decipher the function of SET/TAF-Iα and its contributions to TCE-induced hepatotoxicity, we employed a proteomic analysis of SET/TAF-Iα with tandem affinity purification to identify SET/TAF-Iα-binding proteins. We identified 42 novel Gene Ontology co-annotated SET/TAF-Iα-binding proteins. The identifications of two of these proteins (eEF1A1, elongation factor 1-alpha 1; eEF1A2, elongation factor 1-alpha 2) were confirmed by Western blot analysis and co-immunoprecipitation (Co-IP). Furthermore, we analyzed the effects of TCE on the expression, distribution and interactions of eEF1A1, eEF1A2 and SET in L-02 cells. Western blot analysis reveals a significant up-regulation of eEF1A1, eEF1A2 and two isoforms of SET, and immunocytochemical analysis reveals that eEF1A1 and SET is redistributed by TCE. SET is redistributed from the nucleus to the cytoplasm, while eFE1A1 is translocated from the cytoplasm to the nucleus. Moreover, we find by Co-IP that TCE exposure significantly increases the interaction of SET with eEF1A2. Our data not only provide insights into the physiological functions of SET/TAF-Iα and complement the SET interaction networks, but also demonstrate that TCE exposure induces alterations in the expression, distribution and interactions of SET and its binding partners. These alterations may constitute the mechanisms of TCE cytotoxicity. -- Highlights: ► Identify 62 SET/TAF-Iα-associated proteins in human L-02 cells ► Trichloroethylene (TCE) alters the interaction of SET with eEF1A1 and eEF1A2. ► TCE induces the translocation and up-regulation of SET. ► TCE induces the translocation and up-regulation of eEF1A.

  19. Proteomic analysis of trichloroethylene-induced alterations in expression, distribution, and interactions of SET/TAF-Iα and two SET/TAF-Iα-binding proteins, eEF1A1 and eEF1A2, in hepatic L-02 cells

    International Nuclear Information System (INIS)

    Hong, Wen-Xu; Yang, Liang; Chen, Moutong; Yang, Xifei; Ren, Xiaohu; Fang, Shisong; Ye, Jinbo; Huang, Haiyan; Peng, Chaoqiong; Zhou, Li; Huang, Xinfeng; Yang, Fan; Wu, Desheng; Zhuang, Zhixiong; Liu, Jianjun

    2012-01-01

    Emerging evidence indicates that trichloroethylene (TCE) exposure causes severe hepatotoxicity. However, the mechanisms of TCE hepatotoxicity remain unclear. Recently, we reported that TCE exposure up-regulated the expression of the oncoprotein SET/TAF-Iα and SET knockdown attenuated TCE-induced cytotoxicity in hepatic L-02 cells. To decipher the function of SET/TAF-Iα and its contributions to TCE-induced hepatotoxicity, we employed a proteomic analysis of SET/TAF-Iα with tandem affinity purification to identify SET/TAF-Iα-binding proteins. We identified 42 novel Gene Ontology co-annotated SET/TAF-Iα-binding proteins. The identifications of two of these proteins (eEF1A1, elongation factor 1-alpha 1; eEF1A2, elongation factor 1-alpha 2) were confirmed by Western blot analysis and co-immunoprecipitation (Co-IP). Furthermore, we analyzed the effects of TCE on the expression, distribution and interactions of eEF1A1, eEF1A2 and SET in L-02 cells. Western blot analysis reveals a significant up-regulation of eEF1A1, eEF1A2 and two isoforms of SET, and immunocytochemical analysis reveals that eEF1A1 and SET is redistributed by TCE. SET is redistributed from the nucleus to the cytoplasm, while eFE1A1 is translocated from the cytoplasm to the nucleus. Moreover, we find by Co-IP that TCE exposure significantly increases the interaction of SET with eEF1A2. Our data not only provide insights into the physiological functions of SET/TAF-Iα and complement the SET interaction networks, but also demonstrate that TCE exposure induces alterations in the expression, distribution and interactions of SET and its binding partners. These alterations may constitute the mechanisms of TCE cytotoxicity. -- Highlights: ► Identify 62 SET/TAF-Iα-associated proteins in human L-02 cells ► Trichloroethylene (TCE) alters the interaction of SET with eEF1A1 and eEF1A2. ► TCE induces the translocation and up-regulation of SET. ► TCE induces the translocation and up-regulation of eEF1A.

  20. Understanding the fouling of UF/MF hollow fibres of biologically treated wastewaters using advanced EfOM characterization and statistical tools

    KAUST Repository

    Filloux, Emmanuelle

    2012-08-01

    Five secondary effluents and a river water source were characterized using size exclusion chromatography (LC-OCD-UVD-OND) and emission-excitation matrix (EEM) fluorescence spectroscopy in order to identify the major effluent organic matter (EfOM) fractions responsible for membrane fouling. This study showed the feasibility of coupling fluorescence EEM and LC-OCD-UVD-OND to investigate the fouling potential as well as a means to differentiate natural organic matter (NOM) from EfOM. The secondary effluents and river water showed a significant difference in organic matter characteristics and fouling potential, highlighting the importance of biological processes and the feed water source on EfOM characteristics and fouling potential. On the basis of statistical analysis, protein-like substances were found to be highly correlated to the fouling potential of secondary effluents. © 2012 Elsevier Ltd.

  1. Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type

    DEFF Research Database (Denmark)

    Rose, Adam John; Bisiani, Bruno; Vistisen, Bodil

    2009-01-01

    that the increase in skeletal muscle eEF2 Thr(56) phosphorylation was restricted to type I myofibers. Taken together, these data suggest that the depression of skeletal muscle protein synthesis with endurance-type exercise may be regulated at both initiation (i.e. 4EBP1) and elongation (i.e. eEF2) steps, with eEF2......Protein synthesis in skeletal muscle is known to decrease during exercise and it has been suggested that this may depend on the magnitude of the relative metabolic stress within the contracting muscle. To examine the mechanisms behind this, the effect of exercise intensity on skeletal muscle......) increased during exercise but was not influenced by exercise intensity, and was lower than rest 30min after exercise. On the other hand, 4EBP1 phosphorylation at Thr(37/46) decreased during exercise and this decrease was greater at higher exercise intensities, and was similar to rest 30min after exercise...

  2. Effector region of the translation elongation factor EF-Tu.GTP complex stabilizes an orthoester acid intermediate structure of aminoacyl-tRNA in a ternary complex.

    Science.gov (United States)

    Förster, C; Limmer, S; Zeidler, W; Sprinzl, M

    1994-01-01

    tRNA(Val) from Escherichia coli was aminoacylated with [1-13C]valine and its complex with Thermus thermophilus elongation factor EF-Tu.GTP was analyzed by 13C NMR spectroscopy. The results suggest that the aminoacyl residue of the valyl-tRNA in ternary complex with bacterial EF-Tu and GTP is not attached to tRNA by a regular ester bond to either a 2'- or 3'-hydroxyl group; instead, an intermediate orthoester acid structure with covalent linkage to both vicinal hydroxyls of the terminal adenosine-76 is formed. Mutation of arginine-59 located in the effector region of EF-Tu, a conserved residue in protein elongation factors and the alpha subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins), abolishes the stabilization of the orthoester acid structure of aminoacyl-tRNA. PMID:8183898

  3. Understanding the fouling of UF/MF hollow fibres of biologically treated wastewaters using advanced EfOM characterization and statistical tools

    KAUST Repository

    Filloux, Emmanuelle; Labanowski, Jé rô me; Croue, Jean-Philippe

    2012-01-01

    Five secondary effluents and a river water source were characterized using size exclusion chromatography (LC-OCD-UVD-OND) and emission-excitation matrix (EEM) fluorescence spectroscopy in order to identify the major effluent organic matter (EfOM) fractions responsible for membrane fouling. This study showed the feasibility of coupling fluorescence EEM and LC-OCD-UVD-OND to investigate the fouling potential as well as a means to differentiate natural organic matter (NOM) from EfOM. The secondary effluents and river water showed a significant difference in organic matter characteristics and fouling potential, highlighting the importance of biological processes and the feed water source on EfOM characteristics and fouling potential. On the basis of statistical analysis, protein-like substances were found to be highly correlated to the fouling potential of secondary effluents. © 2012 Elsevier Ltd.

  4. Evaluating the Safety and Tolerability of Sacubitril/Valsartan for HFrEF Managed Within a Pharmacist Clinic.

    Science.gov (United States)

    Pogge, Elizabeth K; Davis, Lindsay E

    2018-04-01

    The objective of this research was to describe the use of pharmacist-managed sacubitril/valsartan therapy in a multi-center, outpatient cardiac group. Sacubitril/valsartan, an angiotensin receptor-neprilysin inhibitor (ARNi), is a novel agent for the treatment of heart failure. An ARNi is recommended by national guidelines to be used in place of angiotensin-converting enzyme inhibitor (ACEi) or angiotensin receptor blocker (ARB) therapy for patients who remain symptomatic. A retrospective chart review was performed to identify patients initiated and fully titrated on sacubitril/valsartan therapy from July 7, 2015 to March 7, 2017. Fifty-two of the 72 symptomatic heart failure with reduced ejection fraction (HFrEF) patients prescribed sacubitril/valsartan during the 21-month period were included in this analysis. The average ejection fraction was 26%. The average age was 69 years. At baseline, 26.9% of patients were not on ACEi/ARB therapy and 13.5% were on target-dose therapy. After completing the uptitration process, the maximally tolerated dose of sacubitril/valsartan was 5.8% low-dose, 7.7% mid-dose, and 86.5% target-dose. Loop and thiazide diuretic use decreased significantly. There was a significant mean reduction in systolic blood pressure of 6 mmHg with no significant changes in serum creatinine, blood urea nitrogen, or potassium levels. With close monitoring and follow-up, ARNi therapy was a safe alternative to ACEi/ARB therapy for chronic symptomatic HFrEF when initiated within a pharmacist clinic.

  5. Proteomic analysis of trichloroethylene-induced alterations in expression, distribution, and interactions of SET/TAF-Iα and two SET/TAF-Iα-binding proteins, eEF1A1 and eEF1A2, in hepatic L-02 cells.

    Science.gov (United States)

    Hong, Wen-Xu; Yang, Liang; Chen, Moutong; Yang, Xifei; Ren, Xiaohu; Fang, Shisong; Ye, Jinbo; Huang, Haiyan; Peng, Chaoqiong; Zhou, Li; Huang, Xinfeng; Yang, Fan; Wu, Desheng; Zhuang, Zhixiong; Liu, Jianjun

    2012-09-01

    Emerging evidence indicates that trichloroethylene (TCE) exposure causes severe hepatotoxicity. However, the mechanisms of TCE hepatotoxicity remain unclear. Recently, we reported that TCE exposure up-regulated the expression of the oncoprotein SET/TAF-Iα and SET knockdown attenuated TCE-induced cytotoxicity in hepatic L-02 cells. To decipher the function of SET/TAF-Iα and its contributions to TCE-induced hepatotoxicity, we employed a proteomic analysis of SET/TAF-Iα with tandem affinity purification to identify SET/TAF-Iα-binding proteins. We identified 42 novel Gene Ontology co-annotated SET/TAF-Iα-binding proteins. The identifications of two of these proteins (eEF1A1, elongation factor 1-alpha 1; eEF1A2, elongation factor 1-alpha 2) were confirmed by Western blot analysis and co-immunoprecipitation (Co-IP). Furthermore, we analyzed the effects of TCE on the expression, distribution and interactions of eEF1A1, eEF1A2 and SET in L-02 cells. Western blot analysis reveals a significant up-regulation of eEF1A1, eEF1A2 and two isoforms of SET, and immunocytochemical analysis reveals that eEF1A1 and SET is redistributed by TCE. SET is redistributed from the nucleus to the cytoplasm, while eFE1A1 is translocated from the cytoplasm to the nucleus. Moreover, we find by Co-IP that TCE exposure significantly increases the interaction of SET with eEF1A2. Our data not only provide insights into the physiological functions of SET/TAF-Iα and complement the SET interaction networks, but also demonstrate that TCE exposure induces alterations in the expression, distribution and interactions of SET and its binding partners. These alterations may constitute the mechanisms of TCE cytotoxicity. Copyright © 2012 Elsevier Inc. All rights reserved.

  6. Impaired left ventricular systolic function reserve limits cardiac output and exercise capacity in HFpEF patients due to systemic hypertension.

    Science.gov (United States)

    Henein, Michael; Mörner, Stellan; Lindmark, Krister; Lindqvist, Per

    2013-09-30

    Heart failure (HF) patients with preserved left ventricular (LV) ejection fraction (EF) (HFpEF) due to systemic hypertension (SHT) are known to have limited exercise tolerance. Despite having normal EF at rest, we hypothesize that these patients have abnormal systolic function reserve limiting their exercise capacity. Seventeen patients with SHT (mean age 68 ± 9 years) but no valve disease and 14 healthy individuals (mean age of 65 ± 10 years) underwent resting and peak exercise echocardiography using conventional, tissue Doppler and speckle tracking techniques. The differences between resting and peak exercise values were also analyzed (Δ). Exercise capacity was determined as the workload divided by body surface area. Resting values for left atrial (LA) volume/BSA (r=-0.66, pexercise capacity. LVEF increased during exercise in normals (mean Δ EF=10 ± 8%) but failed to do so in patients (mean Δ EF=0.6 ± 9%, pexercise in patients, to the same extent as it did in normals (0.2 ± 0.2 vs. 0.6 ± 0.3 1/s, pexercise (Δ) in LV lateral wall systolic velocity from tissue Doppler (s') (0.71, pexercise capacity independent of changes in heart rate. HFpEF patients with hypertensive LV disease have significantly limited exercise capacity which is related to left atrial enlargement as well as compromised LV systolic function at the time of the symptoms. The limited myocardial systolic function reserve seems to be underlying important explanation for their limited exercise capacity. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  7. A randomized study on migration of the Spectron EF and the Charnley flanged 40 cemented femoral components using radiostereometric analysis at 2 years.

    Science.gov (United States)

    Kadar, Thomas; Hallan, Geir; Aamodt, Arild; Indrekvam, Kari; Badawy, Mona; Havelin, Leif Ivar; Stokke, Terje; Haugan, Kristin; Espehaug, Birgitte; Furnes, Ove

    2011-10-01

    We performed a randomized study to determine the migration patterns of the Spectron EF femoral stem and to compare them with those of the Charnley stem, which is regarded by many as the gold standard for comparison of implants due to its extensive documentation. 150 patients with a mean age of 70 years were randomized, single-blinded, to receive either a cemented Charnley flanged 40 monoblock, stainless steel, vaquasheen surface femoral stem with a 22.2-mm head (n = 30) or a cemented Spectron EF modular, matte, straight, collared, cobalt-chrome femoral stem with a 28-mm femoral head and a roughened proximal third of the stem (n = 120). The patients were followed with repeated radiostereometric analysis for 2 years to assess migration. At 2 years, stem retroversion was 2.3° and 0.7° (p < 0.001) and posterior translation was 0.44 mm and 0.17 mm (p = 0.002) for the Charnley group (n = 26) and the Spectron EF group (n = 74), respectively. Subsidence was 0.26 mm for the Charnley and 0.20 mm for the Spectron EF (p = 0.5). The Spectron EF femoral stem was more stable than the Charnley flanged 40 stem in our study when evaluated at 2 years. In a report from the Norwegian arthroplasty register, the Spectron EF stem had a higher revision rate due to aseptic loosening beyond 5 years than the Charnley. Initial stability is not invariably related to good long-term results. Our results emphasize the importance of prospective long-term follow-up of prosthetic implants in clinical trials and national registries and a stepwise introduction of implants.

  8. Polymorphisms in the mitochondrial ribosome recycling factor EF-G2mt/MEF2 compromise cell respiratory function and increase atorvastatin toxicity.

    Directory of Open Access Journals (Sweden)

    Sylvie Callegari

    Full Text Available Mitochondrial translation, essential for synthesis of the electron transport chain complexes in the mitochondria, is governed by nuclear encoded genes. Polymorphisms within these genes are increasingly being implicated in disease and may also trigger adverse drug reactions. Statins, a class of HMG-CoA reductase inhibitors used to treat hypercholesterolemia, are among the most widely prescribed drugs in the world. However, a significant proportion of users suffer side effects of varying severity that commonly affect skeletal muscle. The mitochondria are one of the molecular targets of statins, and these drugs have been known to uncover otherwise silent mitochondrial mutations. Based on yeast genetic studies, we identify the mitochondrial translation factor MEF2 as a mediator of atorvastatin toxicity. The human ortholog of MEF2 is the Elongation Factor Gene (EF-G 2, which has previously been shown to play a specific role in mitochondrial ribosome recycling. Using small interfering RNA (siRNA silencing of expression in human cell lines, we demonstrate that the EF-G2mt gene is required for cell growth on galactose medium, signifying an essential role for this gene in aerobic respiration. Furthermore, EF-G2mt silenced cell lines have increased susceptibility to cell death in the presence of atorvastatin. Using yeast as a model, conserved amino acid variants, which arise from non-synonymous single nucleotide polymorphisms (SNPs in the EF-G2mt gene, were generated in the yeast MEF2 gene. Although these mutations do not produce an obvious growth phenotype, three mutations reveal an atorvastatin-sensitive phenotype and further analysis uncovers a decreased respiratory capacity. These findings constitute the first reported phenotype associated with SNPs in the EF-G2mt gene and implicate the human EF-G2mt gene as a pharmacogenetic candidate gene for statin toxicity in humans.

  9. Microscopia eletrônica de varredura de ovos de Haemagogus leucocelaenus (Diptera: Culicidae Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Jeronimo Alencar

    2003-10-01

    Full Text Available OBJETIVO: Observar detalhes morfológicos de ovos de Haemagogus leucocelaenus visualizados pela primeira vez por microscopia eletrônica de varredura (MEV e realizar morfometria das principais estruturas. MÉTODOS: Foram utilizados ovos de Hg. leucocelaenus provenientes de fêmeas capturadas na Reserva Biológica do Tinguá, RJ, sendo parte destinada à eclosão e outra ao processamento de MEV, dos quais três foram submetidos à análise morfométrica. O material foi fixado em glutaraldeído 2,5% e pós-fixado em tetróxido de ósmio 1%, ambos em tampão cacodilato de sódio 0.1M, pH 7.2, processado e observado ao MEV Jeol 5310. Medições foram realizadas com o auxílio do software de análise Semafore. RESULTADOS: Os ovos apresentaram contorno elíptico com aproximadamente 574 µm de comprimento e 169 µm de largura, sendo o índice do ovo (l/wratio 3,39 µm. O exocório é extremamente regular, possuindo ornamentação hexagonal e algumas vezes pentagonal. Nas células coriônicas, observaram-se tubérculos simetricamente dispostos com relação ao eixo longitudinal, e, no interior delas, tubérculos menores, individualizados, dispostos na periferia, e poucos agrupados no centro. A superfície do retículo coriônico não apresentou rugosidades. O aparelho micropilar apresenta colar proeminente, contínuo, com disco micropilar bem evidente. CONCLUSÕES: A ornamentação do exocório apresenta diferenças em relação aos tubérculos das células coriônicas e ao retículo coriônico externo entre os ovos de Hg. leucocelaenus comparados aos ovos de Hg. janthinomys e Hg. equinus, bem como com relação aos de Aedes aegypti, Ae. albopictus e Ae bahamensis.OBJECTIVE: To observe morphological details of the eggs of Haemagogus (Conopostegus leucocelaenus, seen for the first time via scanning electron microscopy (SEM, with morphometric analysis of the main structures. METHODS: Eggs of Hg. leucocelaenus were obtained from females captured in the

  10. On monitoring unrecorded alcohol consumption

    OpenAIRE

    Rehm, Jürgen; Poznyak, Vladimir

    2015-01-01

    Unrecorded alcohol consumption is a global problem, with about 25% of all alcohol consumption concerning this category. There are different forms of unrecorded alcohol, legally produced versus illegally produced, artisanal vs industrially produced, and then surrogate alcohol, which is officially not intended for human consumption. Monitoring and surveillance of unrecorded consumption is not well developed. The World Health Organization has developed a monitoring system, using the Nominal Grou...

  11. Energy consumption: Past, present, future

    Science.gov (United States)

    1973-01-01

    The energy consumption history of the United States and the changes which could occur in consumption characteristics in the next 50 years are presented. The various sources of energy are analyzed to show the limitations involved in development and utilization as a function of time available. Several scenarios were prepared to show the consumption and supply of energy under varying conditions.

  12. Consumption of the Epidermis

    DEFF Research Database (Denmark)

    Bønnelykke-Behrndtz, Marie Louise; Schmidt, Henrik; Damsgaard, Tine E

    2015-01-01

    It has recently been demonstrated that the extent of ulceration and the presence of epidermal involvement that theoretically precede ulceration (consumption of epidermis, COE) or seen subsequent to inflammation (reactive epidermal hyperplasia or re-epithelialization) allowed better prognostic...... supports that the proliferation drive is noninflammatory. In contrast, the presence of re-epithelialization and/or reactive epidermal hyperplasia demonstrated an 18% (95% CI: 6–53, P = 0.0021) increased density of neutrophils compared with tumor with no evidence of these possibly prolonged late......, effacement, or reactive hyperplasia of the surrounding epidermis....

  13. Proper Islamic Consumption

    DEFF Research Database (Denmark)

    Fischer, Johan

    mobile, religiously committed communities to the opportunities and perils presented by modernisation. It also tells us something about the debates concerning the meanings and practices of Islam within an aggressive, globalised, secularised modernity. In Malaysia this is an especially intriguing issue...... spite of a long line of social theory analyzing the spiritual in the economic, and vice versa, very little of the recent increase in scholarship on Islam addresses its relationship with capitalism. Johan Fischer’s book,Proper Islamic Consumption, begins to fill this gap. […] Fischer’s detailed...

  14. Sustainable Food Consumption

    DEFF Research Database (Denmark)

    Reisch, Lucia; Scholl, Gerd; Eberle, Ulrike

    to be tackled, including climate change water pollution and water scarcity, soil degradation,eutrophication of water bodies, and loss of habitats and biodiversity. With respect to a growing world population and demographic change, problems are predicted to become more serious in the future; for example...... on a variety of issues, including agriculture and the food supply, the availability of and access to food, physical activity, welfare and social benefits, sound environmental production and consumption, fiscal policies, the role of individual consumer decision-making, public procurement and public provision...

  15. A Theory on Fashion Consumption

    OpenAIRE

    Fang Ma; Huijing Shi; Lihua Chen; Yiping Luo

    2012-01-01

    Both the theory of top-down penetration of fashion consumption (Veblen - Simmel model) and the theory of bottom-up fashion consumption have been found consistent with the consumer behavior in the China¡¯s fashion consumer market and the trend of such behavior keeps growing. Therefore, it is necessary to carry out a study on fashion consumption to meet the needs of the development of real life and fashion consumption. Firstly, we describe the content of fashion consumption, discuss the connota...

  16. Population growth and consumption.

    Science.gov (United States)

    Chalkley, K

    1997-04-01

    The relationship between population growth, resource consumption, and environmental degradation is complex. The rise in "greenhouse gases" that will cause climatic change is clearly due to human activity, and pollutants are often concentrated in densely populated areas. However, even an area with a negative population growth, such as Russia, can experience severe environmental degradation due to poor management. Consumption patterns have the most effect on ozone depletion, while population growth threatens biodiversity of and within species through the destruction of ecosystems. Migration joins population growth and social factors, such as land inequality, as major causes of deforestation, and global demand for water is expected to increase faster than the rate of population growth. Coastal development and over-fishing threaten to deplete the oceans, while soil quality is threatened by inappropriate land use. Estimates of the earth's carrying capacity range from less than 3 billion to more than 44 billion people, indicating how difficult it is to assess this figure. Development efforts throughout the world may lead to human gains that will ultimately be negated by environmental losses. These factors have led to growing support for environmentally sustainable development.

  17. Sustainable Consumption Dilemmas

    Directory of Open Access Journals (Sweden)

    Kees Vringer

    2017-06-01

    Full Text Available To examine which considerations play a role when individuals make decisions to purchase sustainable product varieties or not, we have conducted a large scale field experiment with more than 600 participating households. Households can vote on whether the budgets they receive should only be spent on purchasing the sustainable product variety, or whether every household in a group is free to spend their budget on any product variety. By conducting several treatments, we tested whether people tend to view sustainable consumption as a social dilemma or as a moral dilemma. We find little support for the hypothesis that social dilemma considerations are the key drivers of sustainable consumption behaviour. Participants seem to be caught in a moral dilemma in which they not only weigh their individual financial costs with the sustainable benefits but they also consider the consequences of restricting other people’s freedom of choice. Complementary survey results further substantiate this claim and show that many people are reluctant to impose restrictions on their peers, but, at the same time, our results also suggest substantial support for the government to regulate the availability of unsustainable product varieties.

  18. Energy consumption declined in 1993

    International Nuclear Information System (INIS)

    Anon.

    1994-01-01

    On presenting the energy consumption figures for 1993 the Minister for Economic Affairs of Baden-Wuerttemberg Dieter Spoeri (SPD) spoke of the eternal task of saving energy. In his view the slight decline in energy consumption from 1992 to 1993 should not be interpreted as a greater willingness to save energy; its main cause is rather to be seen in the course of the economy. According to estimations, total energy consumption fell 0.5% and electricity consumption 1.0% from 1992 to 1993. The economy on the other hand, still a decisive factor in energy consumption, is estimated to have declined 3% during that period. In the ten years from 1983 to 1993 total energy consumption in the Land rose an average annual 1.8% while electricity consumption kept astride with the economy with an average annual rise 2.7%, he said. (orig./HP) [de

  19. eEF1A Controls ascospore differentiation through elevated accuracy, but controls longevity and fruiting body formation through another mechanism in Podospora anserina.

    Science.gov (United States)

    Silar, P; Lalucque, H; Haedens, V; Zickler, D; Picard, M

    2001-01-01

    Antisuppressor mutations in the eEF1A gene of Podospora anserina were previously shown to impair ascospore formation, to drastically increase life span, and to permit the development of the Crippled Growth degenerative process. Here, we show that eEF1A controls ascospore formation through accuracy level maintenance. Examination of antisuppressor mutant perithecia reveals two main cytological defects, mislocalization of spindle and nuclei and nuclear death. Antisuppression levels are shown to be highly dependent upon both the mutation site and the suppressor used, precluding any correlation between antisuppression efficiency and severity of the sporulation impairment. Nevertheless, severity of ascospore differentiation defect is correlated with resistance to paromomycin. We also show that eEF1A controls fruiting body formation and longevity through a mechanism(s) different from accuracy control. In vivo, GFP tagging of the protein in a way that partly retains its function confirmed earlier cytological observation; i.e., this factor is mainly diffuse within the cytosol, but may transiently accumulate within nuclei or in defined regions of the cytoplasm. These data emphasize the fact that the translation apparatus exerts a global regulatory control over cell physiology and that eEF1A is one of the key factors involved in this monitoring. PMID:11514440

  20. Interaction between the cellular protein eEF1A and the 3'-terminal stem-loop of West Nile virus genomic RNA facilitates viral minus-strand RNA synthesis.

    Science.gov (United States)

    Davis, William G; Blackwell, Jerry L; Shi, Pei-Yong; Brinton, Margo A

    2007-09-01

    RNase footprinting and nitrocellulose filter binding assays were previously used to map one major and two minor binding sites for the cell protein eEF1A on the 3'(+) stem-loop (SL) RNA of West Nile virus (WNV) (3). Base substitutions in the major eEF1A binding site or adjacent areas of the 3'(+) SL were engineered into a WNV infectious clone. Mutations that decreased, as well as ones that increased, eEF1A binding in in vitro assays had a negative effect on viral growth. None of these mutations affected the efficiency of translation of the viral polyprotein from the genomic RNA, but all of the mutations that decreased in vitro eEF1A binding to the 3' SL RNA also decreased viral minus-strand RNA synthesis in transfected cells. Also, a mutation that increased the efficiency of eEF1A binding to the 3' SL RNA increased minus-strand RNA synthesis in transfected cells, which resulted in decreased synthesis of genomic RNA. These results strongly suggest that the interaction between eEF1A and the WNV 3' SL facilitates viral minus-strand synthesis. eEF1A colocalized with viral replication complexes (RC) in infected cells and antibody to eEF1A coimmunoprecipitated viral RC proteins, suggesting that eEF1A facilitates an interaction between the 3' end of the genome and the RC. eEF1A bound with similar efficiencies to the 3'-terminal SL RNAs of four divergent flaviviruses, including a tick-borne flavivirus, and colocalized with dengue virus RC in infected cells. These results suggest that eEF1A plays a similar role in RNA replication for all flaviviruses.

  1. MEDITERRANEAN FOOD CONSUMPTION PATTERNS: LOW ENVIRONMENTAL IMPACTS AND SIGNIFICANT HEALTH AND NUTRITION BENEFITS

    International Nuclear Information System (INIS)

    Elbilali, H.; Capone, R.; Lamaddalena, N.; Lamberti, L.; Elferchichi, A.; Aboussaleh, Y.

    2012-01-01

    Full text: Nutrition is central in the prevention of food-related non-communicable diseases representing an important health risk factor and an enormous socio-economic burden for Mediterranean societies. Nevertheless, assessment of food systems and diets sustainability should take into account not only their health benefits but also their environmental impacts. This work aims at analysing the main environmental impacts of the Mediterranean food consumption patterns (MFCPs) and at highlighting their nutrition and health benefits. The paper provides a review on nutrition and health benefits of the traditional Mediterranean diet (MD) as well as on water and land resources and biodiversity in the Mediterranean. FAO food consumption statistics and standard footprint data were used to characterise the MFCP and to calculate and discuss environmental impacts, i.e. water, carbon and ecological footprints. The Mediterranean hotspot is a major centre of plant and crop diversity. Mediterranean people gather and consume about 2,300 plant species. The share of plant-based energy in the diet is higher in the Mediterranean than in Northern Europe and America. Peoples adhering to the Mediterranean dietary patterns comply better with recommended nutrient and micronutrients intakes. The MD was associated with reduced mortality and lower risk for obesity, type 2 diabetes, cardiovascular diseases and some cancers. During the last decades, the ecological footprint (EF) per capita in the Mediterranean increased while the biocapacity decreased thus the ecological deficit increased. The carbon footprint alone is generally higher than the biocapacity. MENA region has a lower EF than North America. Food consumption represents the highest share of water footprint of consumption (WFC) in the Mediterranean. WFC is lower in Mediterranean countries, especially MENA ones, than in North America. The traditional MD offers considerable health benefits and has lower environmental impacts than Northern

  2. Analysis of transgenic wheat (Triticum aestivum L.) harboring a maize (Zea mays L.) gene for plastid EF-Tu: segregation pattern, expression and effects of the transgene.

    Science.gov (United States)

    Fu, Jianming; Ristic, Zoran

    2010-06-01

    We previously reported that transgenic wheat (Triticum aestivum L.) carrying a maize (Zea mays L.) gene (Zmeftu1) for chloroplast protein synthesis elongation factor, EF-Tu, displays reduced thermal aggregation of leaf proteins, reduced injury to photosynthetic membranes (thylakoids), and enhanced rate of CO(2) fixation following exposure to heat stress (18 h at 45 degrees C) [Fu et al. in Plant Mol Biol 68:277-288, 2008]. In the current study, we investigated the segregation pattern and expression of the transgene Zmeftu1 and determined the grain yield of transgenic plants after exposure to a brief heat stress (18 h at 45 degrees C). We also assessed thermal aggregation of soluble leaf proteins in transgenic plants, testing the hypothesis that increased levels of EF-Tu will lead to a non-specific protection of leaf proteins against thermal aggregation. The transgenic wheat displayed a single-gene pattern of segregation of Zmeftu1. Zmeftu1 was expressed, and the transgenic plants synthesized and accumulated three anti-EF-Tu cross-reacting polypeptides of similar molecular mass but different pI, suggesting the possibility of posttranslational modification of this protein. The transgenic plants also showed better grain yield after exposure to heat stress compared with their non-transgenic counterparts. Soluble leaf proteins of various molecular masses displayed lower thermal aggregation in transgenic than in non-transgenic wheat. The results suggest that overexpression of chloroplast EF-Tu can be beneficial to wheat tolerance to heat stress. Moreover, the results also support the hypothesis that EF-Tu contributes to heat tolerance by acting as a molecular chaperone and protecting heat-labile proteins from thermal aggregation in a non-specific manner.

  3. The utrophin A 5'-UTR drives cap-independent translation exclusively in skeletal muscles of transgenic mice and interacts with eEF1A2.

    Science.gov (United States)

    Miura, P; Coriati, A; Bélanger, G; De Repentigny, Y; Lee, J; Kothary, R; Holcik, M; Jasmin, B J

    2010-04-01

    The molecular mechanisms regulating expression of utrophin A are of therapeutic interest since upregulating its expression at the sarcolemma can compensate for the lack of dystrophin in animal models of Duchenne Muscular Dystrophy (DMD). The 5'-UTR of utrophin A has been previously shown to drive cap-independent internal ribosome entry site (IRES)-mediated translation in response to muscle regeneration and glucocorticoid treatment. To determine whether the utrophin A IRES displays tissue specific activity, we generated transgenic mice harboring control (CMV/betaGAL/CAT) or utrophin A 5'-UTR (CMV/betaGAL/UtrA/CAT) bicistronic reporter transgenes. Examination of multiple tissues from two CMV/betaGAL/UtrA/CAT lines revealed that the utrophin A 5'-UTR drives cap-independent translation of the reporter gene exclusively in skeletal muscles and no other examined tissues. This expression pattern suggested that skeletal muscle-specific factors are involved in IRES-mediated translation of utrophin A. We performed RNA-affinity chromatography experiments combined with mass spectrometry to identify trans-factors that bind the utrophin A 5'-UTR and identified eukaryotic elongation factor 1A2 (eEF1A2). UV-crosslinking experiments confirmed the specificity of this interaction. Regions of the utrophin A 5'-UTR that bound eEF1A2 also mediated cap-independent translation in C2C12 muscle cells. Cultured cells lacking eEF1A2 had reduced IRES activity compared with cells overexpressing eEF1A2. Together, these results suggest an important role for eEF1A2 in driving cap-independent translation of utrophin A in skeletal muscle. The trans-factors and signaling pathways driving skeletal-muscle specific IRES-mediated translation of utrophin A could provide unique targets for developing pharmacological-based DMD therapies.

  4. Loss of translation elongation factor (eEF1A2) expression in vivo differentiates between Wallerian degeneration and dying-back neuronal pathology.

    Science.gov (United States)

    Murray, Lyndsay M; Thomson, Derek; Conklin, Annalijn; Wishart, Thomas M; Gillingwater, Thomas H

    2008-12-01

    Wallerian degeneration and dying-back pathology are two well-known cellular pathways capable of regulating the breakdown and loss of axonal and synaptic compartments of neurons in vivo. However, the underlying mechanisms and molecular triggers of these pathways remain elusive. Here, we show that loss of translation elongation factor eEF1A2 expression in lower motor neurons and skeletal muscle fibres in homozygous Wasted mice triggered a dying-back neuropathy. Synaptic loss at the neuromuscular junction occurred in advance of axonal pathology and by a mechanism morphologically distinct from Wallerian degeneration. Dying-back pathology in Wasted mice was accompanied by reduced expression levels of the zinc finger protein ZPR1, as found in other dying-back neuropathies such as spinal muscular atrophy. Surprisingly, experimental nerve lesion revealed that Wallerian degeneration was significantly delayed in homozygous Wasted mice; morphological assessment revealed that approximately 80% of neuromuscular junctions in deep lumbrical muscles at 24 h and approximately 50% at 48 h had retained motor nerve terminals following tibial nerve lesion. This was in contrast to wild-type and heterozygous Wasted mice where < 5% of neuromuscular junctions had retained motor nerve terminals at 24 h post-lesion. These data show that eEF1A2 expression is required to prevent the initiation of dying-back pathology at the neuromuscular junction in vivo. In contrast, loss of eEF1A2 expression significantly inhibited the initiation and progression of Wallerian degeneration in vivo. We conclude that loss of eEF1A2 expression distinguishes mechanisms underlying dying-back pathology from those responsible for Wallerian degeneration in vivo and suggest that eEF1A2-dependent cascades may provide novel molecular targets to manipulate neurodegenerative pathways in lower motor neurons.

  5. Determination of tumour hypoxia with the PET tracer [18F]EF3: improvement of the tumour-to-background ratio in a mouse tumour model

    International Nuclear Information System (INIS)

    Christian, Nicolas; Bol, Anne; Bast, Marc de; Labar, Daniel; Lee, John; Mahy, Pierre; Gregoire, Vincent

    2007-01-01

    The 2-(2-nitroimidazol-1-yl)-N-(3,3,3-trifluoropropyl)acetamide (EF3) is a 2-nitroimidazole derivative which undergoes bioreductive activation under hypoxic conditions. Using the PET tracer [ 18 F]EF3 in mice, tumour-to-muscle ratios ranging from 1.3 to 3.5 were observed. This study investigated the impact of various interventions aimed at increasing [ 18 F]EF3 elimination, thus potentially increasing the tumour-to-noise ratio in mice, by increasing the renal filtration rate (spironolactone, furosemide), decreasing tubular re-absorption (metronidazole, ornidazole, amino acid solution) or stimulating gastro-intestinal elimination (phenobarbital). C3H mice were injected i.v. with an average of 12.95 MBq of [ 18 F]EF3. Drugs were injected i.v. 15 min before the tracer or daily 4 days prior to the experiment (phenobarbital). Anaesthetised mice were imaged from 30 to 300 min with a dedicated animal PET (Mosaic, Philips). Regions of interest were delineated around the tumour, bladder, heart, liver and leg muscle. Radioactivity was expressed as a percentage of injected activity per gram of tissue. Ornidazole decreased the urinary excretion and increased the liver uptake of [ 18 F]EF3, but without causing any changes in the other organs. Phenobarbital significantly increased the liver concentration and decreased radioactivity in blood and muscle without affecting the tracer uptake in tumour. Consequently, a small but non-significant increase in tumour-to-noise ratio was observed. Although some effects were observed with other drugs, they did not modify the tumour-to-noise ratio. Only phenobarbital induced a trend toward an increased tumour-to-noise ratio that could possibly be tested in the clinical situation. (orig.)

  6. Determination of tumour hypoxia with the PET tracer [{sup 18}F]EF3: improvement of the tumour-to-background ratio in a mouse tumour model

    Energy Technology Data Exchange (ETDEWEB)

    Christian, Nicolas; Bol, Anne; Bast, Marc de; Labar, Daniel; Lee, John; Mahy, Pierre; Gregoire, Vincent [Universite Catholique de Louvain, Center for Molecular Imaging and Experimental Radiotherapy, Brussels (Belgium)

    2007-09-15

    The 2-(2-nitroimidazol-1-yl)-N-(3,3,3-trifluoropropyl)acetamide (EF3) is a 2-nitroimidazole derivative which undergoes bioreductive activation under hypoxic conditions. Using the PET tracer [{sup 18}F]EF3 in mice, tumour-to-muscle ratios ranging from 1.3 to 3.5 were observed. This study investigated the impact of various interventions aimed at increasing [{sup 18}F]EF3 elimination, thus potentially increasing the tumour-to-noise ratio in mice, by increasing the renal filtration rate (spironolactone, furosemide), decreasing tubular re-absorption (metronidazole, ornidazole, amino acid solution) or stimulating gastro-intestinal elimination (phenobarbital). C3H mice were injected i.v. with an average of 12.95 MBq of [{sup 18}F]EF3. Drugs were injected i.v. 15 min before the tracer or daily 4 days prior to the experiment (phenobarbital). Anaesthetised mice were imaged from 30 to 300 min with a dedicated animal PET (Mosaic, Philips). Regions of interest were delineated around the tumour, bladder, heart, liver and leg muscle. Radioactivity was expressed as a percentage of injected activity per gram of tissue. Ornidazole decreased the urinary excretion and increased the liver uptake of [{sup 18}F]EF3, but without causing any changes in the other organs. Phenobarbital significantly increased the liver concentration and decreased radioactivity in blood and muscle without affecting the tracer uptake in tumour. Consequently, a small but non-significant increase in tumour-to-noise ratio was observed. Although some effects were observed with other drugs, they did not modify the tumour-to-noise ratio. Only phenobarbital induced a trend toward an increased tumour-to-noise ratio that could possibly be tested in the clinical situation. (orig.)

  7. Sustainable Consumption: Research Challenges

    DEFF Research Database (Denmark)

    Reisch, Lucia A.; Cohen, Maurie J.; Thøgersen, John

    The Board of the Swedish Foundation for Strategic Environmental Research (Mistra) decided in October 2015 that a proposal for a funding application call in the research area of “sustainable consumption” should be drawn up. According to the statutes of Mistra, research funded by the foundation...... international senior researchers in the eld — Lucia A. Reisch, Maurie J. Cohen, John B. Thøgersen and Arnold Tukker (see Appendix 3) — to draft a background report to prepare the call. The group’s tasks were outlined as follows: ► to describe the challenges facing society in this area, and the political (and...... the orientation of a new research program to be used as draft text for the call for funding applications. The aim of this background report is hence to shed light on future research topics within sustainable consumption from a Swedish perspective. The research pro- moted should help to develop Sweden...

  8. High Antibiotic Consumption

    DEFF Research Database (Denmark)

    Malo, Sara; José Rabanaque, María; Feja, Cristina

    2014-01-01

    Heavy antibiotic users are those individuals with the highest exposure to antibiotics. They play an important role as contributors to the increasing risk of antimicrobial resistance. We applied different methods to identify and characterize the group of heavy antibiotic users in Spain as well...... as their exposure to antibiotics. Data on outpatient prescribing of antimicrobials (ATC J01) in 2010 were obtained from a prescription database covering Aragón (northeastern Spain). The antimicrobial consumption at the individual level was analysed both according to the volume of DDD and the number of packages...... purchased per year. Heavy antibiotic users were identified according to Lorenz curves and characterized by age, gender, and their antimicrobial prescription profile. Lorenz curves demonstrated substantial differences in the individual use of antimicrobials. Heavy antibiotic users (5% of individuals...

  9. RCA : resource consumptions accounting

    OpenAIRE

    Valeska Rodriguez Lucas de Freitas

    2013-01-01

    Devido à grande concorrência do mercado o controle de custos passa a ser peça fundamental no auxílio à tomada de decisão quanto à redução dos custos, visando uma lucratividade maior por parte das empresas. Essa dissertação trata sobre um novo modelo de custos, o RCA – Resource Consumption Accounting, que ainda não foi adotado por nenhuma empresa. Esse novo método de custeio foi aplicado como modelo piloto em uma empresa Americana onde os resultados foram favoráveis a ele em relação aos modelo...

  10. Phylogenetic relationships of true butterflies (Lepidoptera: Papilionoidea) inferred from COI, 16S rRNA and EF-1α sequences.

    Science.gov (United States)

    Kim, Man Il; Wan, Xinlong; Kim, Min Jee; Jeong, Heon Cheon; Ahn, Neung-Ho; Kim, Ki-Gyoung; Han, Yeon Soo; Kim, Iksoo

    2010-11-01

    The molecular phylogenetic relationships among true butterfly families (superfamily Papilionoidea) have been a matter of substantial controversy; this debate has led to several competing hypotheses. Two of the most compelling of those hypotheses involve the relationships of (Nymphalidae + Lycaenidae) + (Pieridae + Papilionidae) and (((Nymphalidae + Lycaenidae) + Pieridae) + Papilionidae). In this study, approximately 3,500 nucleotide sequences from cytochrome oxidase subunit I (COI), 16S ribosomal RNA (16S rRNA), and elongation factor-1 alpha (EF-1α) were sequenced from 83 species belonging to four true butterfly families, along with those of three outgroup species belonging to three lepidopteran superfamilies. These sequences were subjected to phylogenetic reconstruction via Bayesian Inference (BI), Maximum Likelihood (ML), and Maximum Parsimony (MP) algorithms. The monophyletic Pieridae and monophyletic Papilionidae evidenced good recovery in all analyses, but in some analyses, the monophylies of the Lycaenidae and Nymphalidae were hampered by the inclusion of single species of the lycaenid subfamily Miletinae and the nymphalid subfamily Danainae. Excluding those singletons, all phylogenetic analyses among the four true butterfly families clearly identified the Nymphalidae as the sister to the Lycaenidae and identified this group as a sister to the Pieridae, with the Papilionidae identified as the most basal linage to the true butterfly, thus supporting the hypothesis: (Papilionidae + (Pieridae + (Nymphalidae + Lycaenidae))).

  11. O Leviatã eletrônico: a trama política que colocou o Estado na internet The Electronic Leviathan: the political plot to the raising of electronic State

    Directory of Open Access Journals (Sweden)

    Rafael H. Moraes Pereira

    2010-12-01

    Full Text Available As atividades humanas se estão tornando cada vez mais dependentes da internet e, portanto, susceptíveis às suas vulnerabilidades. Entre 1998 e 2002, o governo brasileiro discutiu e implantou a Infraestrutura de Chaves Públicas Brasileira (ICP-Brasil. O objetivo era minimizar a insegurança ao fazer com que a certificação digital, que identifica e autentica pela internet, pudesse operar sobre uma estrutura de criptografia e segurança digital oficial do Estado, com validade jurídica. O livro "O Leviatã Eletrônico: a trama política que colocou o Estado na internet", de Marcello Barra, discute a relação entre Política, Tecnologia e Segurança Digital, ao analisar o processo de criação dessa infraestrutura no Brasil. Com o objetivo de identificar as condições sociais que possibilitaram a efetiva construção da ICP-Brasil, Barra reconstrói os embates técnicos e políticos que precederam esse processo, sem perder de vista a diversidade do conjunto de atores que participaram desse processo. Publicado em 2009, o livro articula a teoria sociológica de diversos autores (como Weber, Elias, Latour, Rosanvallon, Castells, Habermas para discutir surgimento de uma nova manifestação do Estado (o Estado Eletrônico e suas implicações no debate acerca da transparência do setor público, da autoridade do Estado e da Segurança Nacional.Human activities are becoming increasingly dependent on the Internet and, therefore, susceptible to its vulnerabilities. Between 1998 and 2002, the Brazilian government discussed and implemented the ICP-Brasil or, the Brazilian Public Key Infrastructure (Infra-estrutura de Chaves Públicas do Brasil. The aim was to minimize the uncertainty by providing a digital certification - to identify and authenticate in the Internet - that could work with the State's official structure of cryptography and digital security, with legal value. Marcello Barra's book "O Leviatã eletrônico: a trama política que colocou o

  12. Energy consumption and conservation, evaluation

    International Nuclear Information System (INIS)

    Acket, C.

    2006-04-01

    The energy consumption is increasing of more than 1% each year. It is necessary to slow down this growth and much better to inverse it. Observing the main consumption posts, energy saving is possible at short dated for the residential sector and medium and long dated for the transports and the industry. Anyway the individual behaviors are essential. The author presents the situation for each posts, providing data on the energy consumption and saving and recommendations. (A.L.B.)

  13. Solução oxidante gerada a partir da eletrólise de rejeitos de dessalinizadores de água Oxidant solution generated by electrolysis from residue of water desalinators

    Directory of Open Access Journals (Sweden)

    Jeruza Feitosa de Matos

    2006-06-01

    Full Text Available Neste trabalho foram realizados estudos para a produção de solução oxidante a partir de rejeito de dessalinizadores de água pelo processo de eletrólise, visando a sua utilização como desinfetante. O experimento foi realizado em escala de laboratório. Três soluções oxidantes foram geradas eletroliticamente, a partir de rejeitos de dessalinizadores de água com concentrações de 1,7 x 10³ mg; 5,5 x 10³ mg e 10,2 x 10³ mg de Cl-/L. O processo de eletrólise tinha duração de oito horas e, a cada hora da reação, o pH, a condutividade elétrica, a temperatura e o cloro total eram monitorados. Foi observado que a produção de cloro está diretamente relacionada com a concentração de cloreto; o teor médio de cloro das soluções oxidantes geradas foi de 0,10%; 0,24 % e 0,27 % m/m.This work carried out studies about the use of the electrolysis process to produce oxidant solutions from water desalinators wastes for water disinfection. The experiment was conducted in laboratory scale. Three oxidant solutions were generated by electrolysis from wastes of water desalinators that presented concentrations of 1.7 x 10³ mg, 5.5 x 10³ mg and 10.2 x 10³ mg of Cl-/L.The electrolysis process lasted eight hours and the following parameters were monitored every hour: pH, electrical conductivity, temperature, total chlorine. It was observed that the production of chlorine is directly related to chloride concentration; the average content of chlorine of the oxidant solutions generated from the desalinators wastes was 0.10%, 0.24% and 0.27% m/m.

  14. Scanning electron microscopy of the predatory ability of agents of the biocontrol of nematodes/ Microscopia eletrônica de varredura da habilidade predatória de agentes do biocontrole de nematóides

    Directory of Open Access Journals (Sweden)

    João Carlos de Oliveira

    2001-08-01

    Full Text Available Nematode-trapping fungi have been studied as a promising alternative to control bovine gastrointestinal parasites and plant parasitic nematodes. Some encouraging results have been achieved with some species, by many researchers. Nematophagous fungi such as Arthrobotrys javanica, Dactylella leptospora, Monacrosporium eudermatum, M. robustum e Nematoctonus campylosporus were isolated from soil samples collected from different crops and agronomic environments proceeding from varies places in Brazil, making use of soil spreading methods. These fungi species were isolated at the Center of Animal Parasitology (CPPAR at UNESP/FCAV, Campus Jaboticabal – SP. The predatory ability of these fungi, on free living nematodes (Panagrellus sp. was documented at the Scanning Electron Microscopy Lab. The objectives of this work were to document, through Scanning Electron Microscopy (SEM, the morphological structures of these fungi, such as: capturing structures, size, form, septation of the conidia, connection clamp, exclusively on N. campylosporus, as well as the free-living nematodes captured by these fungi.Fungos nematófagos têm sido estudados como uma alternativa promissora para o manejo de nematóides de plantas e parasitos gastrintestinais de bovinos. Resultados encorajadores têm sido obtidos com algumas espécies, por vários pesquisadores. A partir de amostras de solo coletadas em diferentes culturas e agroecossistemas provenientes de diversas localidades do Brasil, foram isolados, pelo método de espalhamento de solo, os fungos nematófagos Arthrobotrys javanica, Dactylella leptospora, Monacrosporium eudermatum, M. robustum e Nematoctonus campylosporus, no Centro de Pesquisas em Sanidade Animal (CPPAR da UNESP/FCAV, Câmpus de Jaboticabal - SP, e a habilidade predatória destes fungos sobre o nematóide de vida livre Panagrellus sp. foi estudada no Laboratório de Microscopia Eletrônica de Varredura. Este trabalho teve como objetivo documentar ao

  15. Evolução da contabilidade brasileira: do governo eletrônico ao sistema público de escrituração digital – SPED - doi: 10.4025/enfoque.v31i2.14603

    Directory of Open Access Journals (Sweden)

    Marcia Sebold

    2012-08-01

    Full Text Available Vivencia-se, na atualidade, uma época de grandes inovações no ramo das tecnologias da informação, e estas têm afetado diversas áreas de atuação profissional e, em particular, a área contábil que vem sofrendo transformações pelo impacto das novas ferramentas computacionais e de comunicação. Com este avanço tecnológico e certificação digital, os Fiscos Federal, Estaduais e Municipais conseguiram criar o Sistema Público de Escrituração Digital (SPED. Este permite o envio de informações tributárias e fiscais de forma segura, rápida e, por meio eletrônico, aos órgãos fiscalizadores. O acesso às informações prestadas pelos contribuintes através do SPED permitirá que as Administrações Fazendárias minimizem o tempo e os recursos despendidos com processos fiscalizatórios. Em contrapartida, os contribuintes também serão beneficiados com a implantação do projeto ora tratado. Diante disto o objetivo deste artigo é apresentar as recentes evoluções da área contábil brasileira, descrevendo os conceitos de contabilidade digital e de governo eletrônico, e destacando o surgimento do Sistema Público de Escrituração Digital (SPED. Trata-se de uma pesquisa qualitativa, na qual os procedimentos metodológicos utilizados na realização deste estudo foram basicamente de natureza bibliográfica, tendo como principais fontes de consulta, legislação, artigos científicos, e sites institucionais. Os resultados deste estudo demonstram as grandes mudanças que vêm ocorrendo nos procedimentos contábeis, inicialmente nas sociedades empresárias sujeitas a acompanhamento econômico-tributário diferenciado, e naquelas tributadas pelo Lucro Real. Ademais, apresentam inúmeros desafios aos profissionais contábeis, a fim de promover ganhos para sociedade, com melhorias na qualidade das informações contábeis e diminuição de práticas fraudulentas.

  16. Utilização do AutoCAD 2004 para quantificação de pesquisas usando fotomicrografias eletrônicas AutoCAD 2004 to quantify electron-photomicrography

    Directory of Open Access Journals (Sweden)

    Carla Albertina Martins Almeida

    2007-08-01

    Full Text Available OBJETIVO: Avaliar o uso do programa AutoCAD 2004 para estudos quantitativos em microscopia eletrônica. MÉTODOS: A demonstração da utilização do AutoCAD 2004 foi feita em um modelo experimental de diabetes induzido em ratos, divididos em Grupo Controle (GC-10 animais e Grupo Diabético (GD - 10 animais, avaliados 1 mês depois da indução do diabetes. Os olhos foram removidos após o sacrifício dos animais e preparados para exame em microscópio eletrônico, tendo sido feitas fotografias dos vasos retinianos. Foi utilizado o programa AutoCAD2004 para avaliar a espessura da membrana basal dos vasos. Os resultados foram avaliados estatisticamente. RESULTADOS: Usando o método proposto foi possível realizar medidas quantitativas na membrana basal de vasos retinianos de ratos diabéticos. CONCLUSÃO: O AutoCAD 2004 se mostrou efetivo, seguro e de fácil utilização para a quantificação de fenômenos biológicos, sendo possível sugerir este meio para a realização de avaliações quantitativas em experimentos biológicos.OBJECTIVE: To determine if the AutoCAD 2004 program is available to be used in quantitative research on electron microscopy. METHODS: A demonstrative of the AutoCAD2004 was done in an experimental model of diabetes induced in rats. The animals were divided by lot in control group animals (GC-10 animals and diabetic group (GD - 10 animals, evaluated 1 month after the diabetes induction. The eyes were removed after sacrifice and prepared to electron microscopy. The retina was photographed and the AutoCAD2004 program was used to measure the basal membrane of retina vessels. The results were submitted to statistical analysis. RESULTS: The AutoCAD2004 was effective to obtain quantitative measures from the basal membrane of retina vessels and there were no difference between the studied groups. CONCLUSION: The AutoCAD2004 was effective, safe and easy to apply to quantify biological parameters, which allowed suggest it to

  17. Uma metodologia bayesiana para estudos de confiabilidade na fase de projeto: aplicação em um produto eletrônico A bayesian methodology for reliability studies in the design phase: application to an electronic product

    Directory of Open Access Journals (Sweden)

    Ruth Myriam Ramírez Pongo

    1997-12-01

    Full Text Available Hoje em dia as indústrias têm de colocar seus produtos em um mercado altamente competitivo, e as pressões, visando eficiência do programa de testes e do ciclo de desenvolvimento de um produto, são cada vez maiores. Neste contexto, os métodos estatísticos clássicos (baseados na teoria de amostragem, usados para analisar a confiabilidade de um produto, mostram-se cada vez menos eficientes, enquanto que os métodos bayesianos são vistos favoravelmente e seu uso começa a ser uma alternativa necessária. Para avaliar o desempenho de um item, que possui uma taxa de falhas muito baixa, as técnicas estatísticas clássicas exigem tamanhos grandes de amostra e tempo de teste longo, principalmente quando a tecnologia do produto limita o fator de aceleração, como é o caso dos produtos eletrônicos, por exemplo. A metodologia, proposta neste artigo, combina resultados de testes, que são realizados rotineiramente durante o ciclo de desenvolvimento, com informação adicional relevante e útil ao estabelecimento da confiabilidade do equipamento. Com o objetivo de ilustrar a metodologia, esta é aplicada em um equipamento eletrônico, cuja confiabilidade foi estabelecida, durante sua fase de projeto, a partir das confiabilidades de seus componentes, utilizando dados de teste por atributo e incorporando o julgamento da equipe de desenvolvimento do produto.Nowadays industries have to place their products in a highly competitive market, and there have been increasing pressures on efficiency test programs and product development cycles. In this context, the classical statistical methods (based on sampling theory, which are used to analyze product reliability, are negatively affected, while the bayesian methods are favorably regarded, and their use is becoming a necessary alternative. In order to evaluate the performance of an item with a very low failure rate, the classical statistical techniques require a large sample size and a long test time

  18. On monitoring unrecorded alcohol consumption

    Directory of Open Access Journals (Sweden)

    Jürgen Rehm

    2015-06-01

    Full Text Available Unrecorded alcohol consumption is a global problem, with about 25% of all alcohol consumption concerning this category. There are different forms of unrecorded alcohol, legally produced versus illegally produced, artisanal vs industrially produced, and then surrogate alcohol, which is officially not intended for human consumption. Monitoring and surveillance of unrecorded consumption is not well developed. The World Health Organization has developed a monitoring system, using the Nominal Group Technique, a variant of the Delphi methodology. Experiences with this methodology over the past two years are reported. Finally, conclusions for the monitoring and surveillance at the national level are given.

  19. Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin.

    Science.gov (United States)

    Kasinski, Andrea L; Du, Yuhong; Thomas, Shala L; Zhao, Jing; Sun, Shi-Yong; Khuri, Fadlo R; Wang, Cun-Yu; Shoji, Mamoru; Sun, Aiming; Snyder, James P; Liotta, Dennis; Fu, Haian

    2008-09-01

    The nuclear factor-kappaB (NF-kappaB) signaling pathway has been targeted for therapeutic applications in a variety of human diseases, includuing cancer. Many naturally occurring substances, including curcumin, have been investigated for their actions on the NF-kappaB pathway because of their significant therapeutic potential and safety profile. A synthetic monoketone compound termed 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24) was developed from curcumin and exhibited potent anticancer activity. Here, we report a mechanism by which EF24 potently suppresses the NF-kappaB signaling pathway through direct action on IkappaB kinase (IKK). We demonstrate that 1) EF24 induces death of lung, breast, ovarian, and cervical cancer cells, with a potency about 10 times higher than that of curcumin; 2) EF24 rapidly blocks the nuclear translocation of NF-kappaB, with an IC(50) value of 1.3 microM compared with curcumin, with an IC(50) value of 13 microM; 3) EF24 effectively inhibits tumor necrosis factor (TNF)-alpha-induced IkappaB phosphorylation and degradation, suggesting a role of this compound in targeting IKK; and 4) EF24 indeed directly inhibits the catalytic activity of IKK in an in vitro-reconstituted system. Our study identifies IKK as an effective target for EF24 and provides a molecular explanation for a superior activity of EF24 over curcumin. The effective inhibition of TNF-alpha-induced NF-kappaB signaling by EF24 extends the therapeutic application of EF24 to other NF-kappaB-dependent diseases, including inflammatory diseases such as rheumatoid arthritis.

  20. Sociology of Drug Consumption

    Directory of Open Access Journals (Sweden)

    2004-02-01

    Full Text Available In this article which is a review of sociological ideas and studies of drug abusers in social situation, drug addiction steps (particularly alcohol, heroin and cocaine consumption are revised and some explanations are made. Also, the role of some sociological ideas in drug addiction is considered in which Anomie Theory reads: "because of such duality, the individuals who are not satisfied with their role are in hurt." According to this theory, drug users choose seclusion and neglecting usual social aims as well as competitive situations. Association of Differentiation Theory claims that drug use behavior is a learned behavior and the first learning occurs in a friendly small group (i.e. youngsters. Social Control theory believes that one can predict normal and abnormal behaviors through the rate of individuals' social commitments. Internal and external controls also determine commitment rate. Micro-cultural theory considers drug use as a compatibility with abnormal micro-culture rules. Symbolic Mutual Action Believes that the etiquettes which society attribute to individuals/behaviors determine their acquired social reactions rather than any inherited acquisition.

  1. Survey on food consumption

    International Nuclear Information System (INIS)

    Ohmomo, Yoichiro

    1979-01-01

    Critical pathway approach is one of the methods for estimating discharge limits of radioactive nuclides into an environment. In connection with this it is presented that a term of 'critical group' is in danger of leading to the misunderstanding of 'dangerous group'. However, in fact, the present discharge is limited to a level as low as practicable. Therefore, on the evaluation of intermal radiation dose for such a low release, a term of 'reference group' should be recommended instead of critical group. In order to select reasonably 'the reference group', it was proposed to calculate 'effective whole body dose-equivalent limit', which was recommended by ICRP publication 26. It is a convenient method to sum up individual weighed dose of each critical organ by using the value of a ratio obtained from Wt as a weighing factor. Finally instructions for the survey on food consumption and the results were summarized on the basis of ten-years survey experiences in coastal area of Ibaraki prefecture. (author)

  2. Site of ADP-ribosylation and the RNA-binding site are situated in different domains of the elongation factor EF-2

    International Nuclear Information System (INIS)

    Davydova, E.K.

    1987-01-01

    One of the proteins participating in the process of elongation of polypeptide chains - elongation factor 2 (EF-2) - can be ADP-ribosylated at a unique amino acid residue - diphthamide. Since the ADP-ribosylation of EF-2 at dipthamide leads to a loss of affinity of the factor for RNA while the presence of RNA inhibits the ADP-ribosylation reaction, it seemed probable to the authors that diphthamide participated directly in the binding of EF-2 to DNA. The experiments presented in this article showed that this was not the case: diphthamide and the RNA-binding site are situated on different domains of EF-2. Thus, ADP-ribosylation of factor EF-2 in one domain leads to a loss of the ability to bind to RNA in the other. The authors investigated the mutual arrangement of diphthamide and the RNA-binding site on the EF-2 molecule by preparing a factor from rabbit reticulocytes and subjecting it to proteolytic digestion with elastase. The factor was incubated with elastase for 15 min at 37 0 C at an enzyme:substrate ratio of 1:100 in buffer solution containing 20 mM Tris-HCl, pH 7.6, 10 mM KCl, 1 mM MgCl 2 , and 2 mM dithiothreitol. The reaction was stopped by adding para-methylsulfonyl fluoride to 50 micro-M. The authors obtained a preparation as a result of proteolysis and applied it on a column with RNA-Sepharose and separated into two fractions: RNA-binding and without affinity for RNA. The initial preparation and its fractions were subjected to exhaustive ADP-ribosylation in the presence of diphtheria toxin and [U- 14 C] nicotinaide adenine dinucleotide ([ 14 C]NAD) (296 mCi/mmole). The samples were analyzed electrophoretically in a polyacrylamide gel gradient in the presence of sodium dodecyl sulfate. For the detection of [ 14 C] ADP-ribosylated components, the gels were dried and exposed with RM-V x-ray film

  3. Klucel™ EF and ELF polymers for immediate-release oral dosage forms prepared by melt extrusion technology.

    Science.gov (United States)

    Mohammed, Noorullah Naqvi; Majumdar, Soumyajit; Singh, Abhilasha; Deng, Weibin; Murthy, Narasimha S; Pinto, Elanor; Tewari, Divya; Durig, Thomas; Repka, Michael A

    2012-12-01

    The objective of this research work was to evaluate Klucel™ hydroxypropylcellulose (HPC) EF and ELF polymers, for solubility enhancement as well as to address some of the disadvantages associated with solid dispersions. Ketoprofen (KPR), a Biopharmaceutics Classification System class II drug with poor solubility, was utilized as a model compound. Preliminary thermal studies were performed to confirm formation of a solid solution/dispersion of KPR in HPC matrix and also to establish processing conditions for hot-melt extrusion. Extrudates pelletized and filled into capsules exhibited a carrier-dependent release with ELF polymer exhibiting a faster release. Tablets compressed from milled extrudates exhibited rapid release owing to the increased surface area of the milled extrudate. Addition of mannitol (MNT) further enhanced the release by forming micro-pores and increasing the porosity of the extrudates. An optimized tablet formulation constituting KPR, MNT, and ELF in a 1:1:1 ratio exhibited 90% release in 15 min similar to a commercial capsule formulation. HPC polymers are non-ionic hydrophilic polymers that undergo polymer-chain-length-dependent solubilization and can be used to enhance solubility or dissolution rate of poorly soluble drugs. Dissolution/release rate could be tailored for rapid-release applications by selecting a suitable HPC polymer and altering the final dosage form. The release obtained from pellets was carrier-dependent and not drug-dependent, and hence, such a system can be effectively utilized to address solubility or precipitation issues with poorly soluble drugs in the gastrointestinal environment.

  4. Frontiers in sustainable consumption research

    DEFF Research Database (Denmark)

    Reisch, Lucia A.; Cohen, Maurie J.; Thøgersen, John

    2016-01-01

    While the field of sustainable consumption research is relatively young, it has already attracted scholars from all corners of the social sciences. The time has come to identify a new research agenda as trends in sustainable consumption research seem to suggest the dawning of a new phase. Not only...

  5. Canonical Authors in Consumption Theory

    DEFF Research Database (Denmark)

    Canonical Authors in Consumption Theory is the first work to compile the contributions of the greatest social thinkers in the global conversation about consumption and consumer culture. A prestigious reference work, it offers original chapters by the world's most prominent thought leaders and sur...

  6. Sustainable Consumption and Life Satisfaction

    Science.gov (United States)

    Xiao, Jing Jian; Li, Haifeng

    2011-01-01

    The purpose of this study was to examine the association between sustainable consumption and life satisfaction. One aspect of sustainable consumption focused on in this study is the environment friendly purchase or green purchase. Using data collected from consumers in 14 cities in China, we found that consumers who reported green purchase…

  7. Second-home electricity consumption

    DEFF Research Database (Denmark)

    Møller Andersen, Frits; Christensen, M.S.; Jensen, O.M.

    2008-01-01

    in electricity consumption. Luxury tourism use and senior citizens' that use a few per cent of the second homes as their home contribute to a minor degree to the overall increase of electricity consumption. Scenarios show that this development may accelerate with increased leisure time, increased use and more...

  8. Second-home electricity consumption

    Energy Technology Data Exchange (ETDEWEB)

    Andersen, Frits M.; Morthorst, Poul Erik [Risoe, Systems Analysis Department, Technical University of Denmark, P.O. Box 49, DK-4000 Roskilde (Denmark); Christensen, Morten S.; Kofoed, Niels-Ulrik [Esbensen Consulting Engineers, Carl Jacobsens Vej 25D, DK-2500 Valby (Denmark); Jensen, Ole Michael [Danish Building Research Institute, Department of Energy and Environment, University of Aalborg, P.O. Box 119, DK-2970 Horsholm (Denmark)

    2008-01-15

    In Denmark, electricity consumption in first and second homes has developed quite differently. Since 1990, electricity consumption in ordinary residences has grown moderately, while consumption in weekend and second homes has increased considerably. In turn, this development has been blamed on a growing number of luxury cottages, new legislation permitting senior citizens to have their permanent address in their second home and a growing number of electric appliances. In order to examine the growing electricity consumption in second homes and to estimate future demand, a multidisciplinary study combining top-down and bottom-up analyses was conducted, i.e., combining models using aggregated economic parameters and feasibility studies using technical parameters, respectively. The top-down estimation showed that changes in electricity consumption in second homes correlate to changes in income. The bottom-up estimation showed that consumption was mainly affected by the frequency with which second homes were used in the winter time. This indicates that additional second homes, increased full-time use and intensified use of electric appliances are the main reasons for the observed increases in electricity consumption. Luxury tourism use and senior citizens' that use a few per cent of the second homes as their home contribute to a minor degree to the overall increase of electricity consumption. Scenarios show that this development may accelerate with increased leisure time, increased use and more permanent settlement in second homes. (author)

  9. Second-home electricity consumption

    International Nuclear Information System (INIS)

    Andersen, Frits M.; Christensen, Morten S.; Jensen, Ole Michael; Kofoed, Niels-Ulrik; Morthorst, Poul Erik

    2008-01-01

    In Denmark, electricity consumption in first and second homes has developed quite differently. Since 1990, electricity consumption in ordinary residences has grown moderately, while consumption in weekend and second homes has increased considerably. In turn, this development has been blamed on a growing number of luxury cottages, new legislation permitting senior citizens to have their permanent address in their second home and a growing number of electric appliances. In order to examine the growing electricity consumption in second homes and to estimate future demand, a multidisciplinary study combining top-down and bottom-up analyses was conducted, i.e., combining models using aggregated economic parameters and feasibility studies using technical parameters, respectively. The top-down estimation showed that changes in electricity consumption in second homes correlate to changes in income. The bottom-up estimation showed that consumption was mainly affected by the frequency with which second homes were used in the winter time. This indicates that additional second homes, increased full-time use and intensified use of electric appliances are the main reasons for the observed increases in electricity consumption. Luxury tourism use and senior citizens' that use a few per cent of the second homes as their home contribute to a minor degree to the overall increase of electricity consumption. Scenarios show that this development may accelerate with increased leisure time, increased use and more permanent settlement in second homes

  10. O impacto da exclusão digital na utilização potencial de um mercado eletrónico de serviços de cuidados de saúde e serviços sociais

    Directory of Open Access Journals (Sweden)

    Maria Manuela Cruz-Cunha

    2014-12-01

    Full Text Available Cada vez mais os indivíduos necessitam de competências digitais avançadas para participar plenamente na sociedade. Numa Europa em crescente envelhecimento é atualmente reconhecida a importância e o potencial da indústria de serviços para envelhecer bem baseados nas tecnologias de informação e de comunicação (TIC, de que é exemplo o mercado eletrónico de serviços sociais e de cuidados de saúde, o GuiMarket, proposto pelos autores para um município do Norte de Portugal. Com base nos resultados de um inquérito realizado junto de uma amostra de 315 indivíduos, o artigo discute a importância reconhecida a tal serviço e a frequência de utilização prevista, concluindo existir uma íntima relação entre o acesso às TIC e a utilização que os inquiridos preveem fazer do GuiMarket.

  11. Lipofuscinose ceróide neuronal: relato de quatro casos com estudo por histoquímica retal, microscopia eletrônica de conjuntiva e necrópsia

    Directory of Open Access Journals (Sweden)

    Luiz Fernando Bleggi Torres

    1994-03-01

    Full Text Available Os autores relatam os achados neuropatológicos e clínicos de quatro casos de lipofuscinose ceróide ou doença de Batten. Dois casos foram estudados por necrópsia: o encéfalo mostrou atrofia e neurônios contendo pigmento citoplasmático com características tintoriais de lipofuscina. Um caso foi diagnosticado por estudo histoquímico de biópsia retal, em material congelado em nitrogênio líquido demonstrando-se células ganglionares mioentéricas contendo acúmulo citoplasmático de material granular fosfatase ácida positivo, bem como em grande número de macrófagos do córion. O quarto caso foi diagnosticado por microscopia eletrônica de biópsia de conjuntiva, com identificação de inclusões curvelíneas membranáceas e tipo impressão digital.

  12. Uma análise crítica do edital JogosBR 2004 para a compreensão do mercado brasileiro de jogos eletrônicos

    Directory of Open Access Journals (Sweden)

    Jônatas Kerr de Oliveira

    2012-12-01

    Full Text Available O edital JogosBr de 2004 é um marco histórico na indústria do videogame no Brasil, e reflete diversos problemas mercadológicos, econômicos e sociais que remontam à reserva de mercado dos anos 80. Este artigo parte de uma análise crítica do edital JogosBR de 2004, um mecanismo de incentivo à produção de jogos em território nacional por parte do governo federal, para realizar uma reflexão sobre o setor de jogos no Brasil e no mundo. Para contextualizar o edital e reforçar sua importância, este artigo aborda a formação da indústria de jogos eletrônicos no país, e sua trajetória até o lançamento do edital, momento em que a indústria brasileira estava fortemente debilitada. A partir desta análise histórica e contextual, o edital é analisado em seu regulamento, nos projetos aprovados e nos resultados obtidos por ele, concluindo com um levantamento de problemas e falhas ocorridos na concepção e execução deste e sugestões para futuros concursos.

  13. Perfil do paciente albino com visão subnormal e melhoria da acuidade visual com a adaptação de recursos ópticos e/ou eletrônicos

    Directory of Open Access Journals (Sweden)

    Diego Fleury de Lemos Pereira

    Full Text Available RESUMO Objetivo: Determinar o perfil do paciente albino no departamento de visão subnormal do Instituto Benjamin Constant. Ressaltar o tempo de acompanhamento, a frequência do seguimento, e a melhora visual com a adaptação de recursos ópticos e/ou eletrônicos. Métodos: Estudo retrospectivo com dados de 77 pacientes albinos com idade entre 1 a 53 anos de idade atendidos no Instituto Benjamin Constant, entre 2003 e 2014. Resultados: O recurso óptico mais adaptado foi o telescópio de Galilleu 2.8x. Todos os pacientes referiram ganho de visão com os equipamentos. A maioria dos pacientes apresentaram acuidade visual com recursos ópticos entre 20/25 e 20/160. Conclusão: Os recursos ópticos auxiliaram na melhora da função visual e na qualidade de vida dos pacientes com albinismo ocular.

  14. A consumption value-gap analysis for sustainable consumption.

    Science.gov (United States)

    Biswas, Aindrila

    2017-03-01

    Recent studies on consumption behavior have depicted environmental apprehension resulting from across wide consumer segments. However, this has not been widely reflected upon the growth in the market shares for green or environment-friendly products mostly because gaps exist between consumers' expectations and perceptions for those products. Previous studies have highlighted the impact of perceived value on potential demand, consumer satisfaction and behavioral intentions. The necessity to understand the effects of gaps in expected and perceived values on consumers' behavioral intention and potential demand for green products cannot be undermined as it shapes the consumers' inclination to repeated purchase and consumption and thus foster potential market demand. Pertaining to this reason, the study aims to adopt a consumption value-gap model based on the theory of consumption values to assess their impact on sustainable consumption behavior and market demand of green products. Consumption value refers to the level of fulfillment of consumer needs by assessment of net utility derived after effective comparison between the benefits (financial or emotional) and the gives (money, time, or energy). The larger the gaps the higher will be the adversarial impact on behavioral intentions. A structural equation modeling was applied to assess data collected through questionnaire survey. The results indicate that functional value-gap and environmental value-gap has the most adversarial impact on sustainable consumption behavior and market demand for green products.

  15. Cloning, sequencing, and transgenic expression of Podospora curvicolla and Sordaria macrospora eEF1A genes: relationship between cytosolic translation and longevity in filamentous fungi.

    Science.gov (United States)

    Gagny, B; Rossignol, M; Silar, P

    1997-12-01

    We have cloned and sequenced the gene encoding the translation elongation factor eEF1A from two filamentous fungi, Podospora curvicolla and Sordaria macrospora. These fungi are close relatives of Podospora anserina and also show senescence syndromes. Comparison of the sequences of the deduced proteins with that of P. anserina reveals that the three proteins differ in several positions. Replacement of the P. anserina gene by either of the two exogenous genes does not entail any modification in P. anserina physiology; the longevity of the fungus is not affected. No alteration of in vivo translational accuracy was detected; however, the exogenous proteins nonetheless promoted a modification of the resistance to the aminoglycoside antibiotic paromomycin. These data suggest that optimization of life span between these closely related fungi has likely not been performed during evolution through modifications of eEF1A activity, despite the fact that mutations in this factor can drastically affect longevity. Copyright 1997 Academic Press.

  16. 15N NMR relaxation studies of calcium-loaded parvalbumin show tight dynamics compared to those of other EF-hand proteins

    DEFF Research Database (Denmark)

    Baldellon, C; Alattia, J R; Strub, M P

    1998-01-01

    Dynamics of the rat alpha-parvalbumin calcium-loaded form have been determined by measurement of 15N nuclear relaxation using proton-detected heteronuclear NMR spectroscopy. The relaxation data were analyzed using spectral density functions and the Lipari-Szabo formalism. The major dynamic features...... for the rat alpha-parvalbumin calcium-loaded form are (1) the extreme rigidity of the helix-loop-helix EF-hand motifs and the linker segment connecting them, (2) the N and C termini of the protein being restricted in their mobility, (3) a conformational exchange occurring at the kink of helix D, and (4...... properties which are conserved in the EF-hand domains from different members of this superfamily: (1) a tendency toward higher mobility of NH vectors at relative position 2 in the Ca2+-binding loop, (2) a restricted mobility for the other residues in the binding loop, and (3) an overall rigidity...

  17. Spitzer IRS Spectroscopy of the 10 Myr-Old EF Cha Debris Disk: Evidence for Phyllosilicate-Rich Dust in the Terrestrial Zone

    Science.gov (United States)

    Currie, Thayne; Lisse, Carey M.; Sicillia-Aguilar, Aurora; Rieke, George H.; Su, Kate Y. L.

    2011-01-01

    We describe Spitzer IRS spectroscopic observations of the approx. 10 Myr-old star, EF Chao Compositional modeling of the spectra from 5 micron to 35 micron confirms that it is surrounded by a luminous debris disk with L(sub D)/L(sub *) approx. 10(exp -3), containing dust with temperatures between 225 K and 430 K characteristic of the terrestrial zone. The EF Cha spectrum shows evidence for many solid-state features, unlike most cold, low-luminosity debris disks but like some other 10-20 Myr-old luminous, warm debris disks (e.g. HD 113766A). The EF Cha debris disk is unusually rich in a species or combination of species whose emissivities resemble that of finely-powdered, laboratory-measured phyllosilicate species (talc, saponite, and smectite), which are likely produced by aqueous alteration of primordial anhydrous rocky materials. The dust and, by inference, the parent bodies of the debris also contain abundant amorphous silicates and metal sulfides, and possibly water ice. The dust's total olivine to pyroxene ratio of approx. 2 also provides evidence of aqueous alteration. The large mass volume of grains with sizes comparable to or below the radiation blow-out limit implies that planetesimals may be colliding at a rate high enough to yield the emitting dust but not so high as to devolatize the planetesimals via impact processing. Because phyllosilicates are produced by the interactions between anhydrous rock and warm, reactive water, EF Cha's disk is a likely signpost for water delivery to the terrestrial zone of a young planetary system.

  18. Synthesis of the EF-ring of ciguatoxin 3C based on the [2,3]-Wittig rearrangement and ring-closing olefin metathesis.

    Science.gov (United States)

    Goto, Akiyoshi; Fujiwara, Kenshu; Kawai, Ayako; Kawai, Hidetoshi; Suzuki, Takanori

    2007-12-20

    The EF-ring segment of ciguatoxin 3C, a causative toxin of ciguatera fish poisoning, was synthesized in three major steps: 1,4-addition for the C20O-C27 bond connection, chirality transferring anti selective [2,3]-Wittig rearrangement for the construction of the anti-2-hydroxyalkyl ether part, and ring-closing olefin metathesis for the F-ring formation.

  19. Heterologous expression of a plastid EF-Tu reduces protein thermal aggregation and enhances CO2 fixation in wheat (Triticum aestivum) following heat stress.

    Science.gov (United States)

    Fu, Jianming; Momcilović, Ivana; Clemente, Thomas E; Nersesian, Natalya; Trick, Harold N; Ristic, Zoran

    2008-10-01

    Heat stress is a major constraint to wheat production and negatively impacts grain quality, causing tremendous economic losses, and may become a more troublesome factor due to global warming. At the cellular level, heat stress causes denaturation and aggregation of proteins and injury to membranes leading to alterations in metabolic fluxes. Protein aggregation is irreversible, and protection of proteins from thermal aggregation is a strategy a cell uses to tolerate heat stress. Here we report on the development of transgenic wheat (Triticum aestivum) events, expressing a maize gene coding for plastidal protein synthesis elongation factor (EF-Tu), which, compared to non-transgenic plants, display reduced thermal aggregation of leaf proteins, reduced heat injury to photosynthetic membranes (thylakoids), and enhanced rate of CO(2) fixation after exposure to heat stress. The results support the concept that EF-Tu ameliorates negative effects of heat stress by acting as a molecular chaperone. This is the first demonstration of the introduction of a plastidal EF-Tu in plants that leads to protection against heat injury and enhanced photosynthesis after heat stress. This is also the first demonstration that a gene other than HSP gene can be used for improvement of heat tolerance and that the improvement is possible in a species that has a complex genome, hexaploid wheat. The results strongly suggest that heat tolerance of wheat, and possibly other crop plants, can be improved by modulating expression of plastidal EF-Tu and/or by selection of genotypes with increased endogenous levels of this protein.

  20. Using docking and alchemical free energy approach to determine the binding mechanism of eEF2K inhibitors and prioritizing the compound synthesis.

    Science.gov (United States)

    Wang, Qiantao; Edupuganti, Ramakrishna; Tavares, Clint D J; Dalby, Kevin N; Ren, Pengyu

    2015-01-01

    A-484954 is a known eEF2K inhibitor with submicromolar IC50 potency. However, the binding mechanism and the crystal structure of the kinase remains unknown. Here, we employ a homology eEF2K model, docking and alchemical free energy simulations to probe the binding mechanism of eEF2K, and in turn, guide the optimization of potential lead compounds. The inhibitor was docked into the ATP-binding site of a homology model first. Three different binding poses, hypothesis 1, 2, and 3, were obtained and subsequently applied to molecular dynamics (MD) based alchemical free energy simulations. The calculated relative binding free energy of the analogs of A-484954 using the binding pose of hypothesis 1 show a good correlation with the experimental IC50 values, yielding an r (2) coefficient of 0.96 after removing an outlier (compound 5). Calculations using another two poses show little correlation with experimental data, (r (2) of less than 0.5 with or without removing any outliers). Based on hypothesis 1, the calculated relative free energy suggests that bigger cyclic groups, at R1 e.g., cyclobutyl and cyclopentyl promote more favorable binding than smaller groups, such as cyclopropyl and hydrogen. Moreover, this study also demonstrates the ability of the alchemical free energy approach in combination with docking and homology modeling to prioritize compound synthesis. This can be an effective means of facilitating structure-based drug design when crystal structures are not available.

  1. The mazEF toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis.

    Science.gov (United States)

    Soheili, Sara; Ghafourian, Sobhan; Sekawi, Zamberi; Neela, Vasantha Kumari; Sadeghifard, Nourkhoda; Taherikalani, Morovat; Khosravi, Afra; Ramli, Ramliza; Hamat, Rukman Awang

    2015-01-01

    The toxin-antitoxin (TA) system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. In this study, the prevalence of TA systems in independently isolated clinical isolates of Enterococcus faecium and Enterococcus faecalis was determined, the dominant TA system was identified, different virulence genes in E. faecium and E. faecalis were surveyed, the level of expression of the virulence and TA genes in normal and stress conditions was determined, and finally their associations with the TA genes were defined. Remarkably, the analysis demonstrated higBA and mazEF in all clinical isolates, and their locations were on chromosomes and plasmids, respectively. On the other hand, a quantitative analysis of TA and virulence genes revealed that the expression level in both genes is different under normal and stress conditions. The results obtained by anti-mazF peptide nucleic acids demonstrated that the expression level of virulence genes had decreased. These findings demonstrate an association between TA systems and virulence factors. The mazEF on the plasmids and the higBA TA genes on the chromosomes of all E. faecium and E. faecalis strains were dominant. Additionally, there was a decrease in the expression of virulence genes in the presence of anti-mazF peptide nucleic acids. Therefore, it is suggested that mazEF TA systems are potent and sensitive targets in all E. faecium and E. faecalis strains.

  2. The mazEF toxin–antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis

    Science.gov (United States)

    Soheili, Sara; Ghafourian, Sobhan; Sekawi, Zamberi; Neela, Vasantha Kumari; Sadeghifard, Nourkhoda; Taherikalani, Morovat; Khosravi, Afra; Ramli, Ramliza; Hamat, Rukman Awang

    2015-01-01

    The toxin–antitoxin (TA) system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. In this study, the prevalence of TA systems in independently isolated clinical isolates of Enterococcus faecium and Enterococcus faecalis was determined, the dominant TA system was identified, different virulence genes in E. faecium and E. faecalis were surveyed, the level of expression of the virulence and TA genes in normal and stress conditions was determined, and finally their associations with the TA genes were defined. Remarkably, the analysis demonstrated higBA and mazEF in all clinical isolates, and their locations were on chromosomes and plasmids, respectively. On the other hand, a quantitative analysis of TA and virulence genes revealed that the expression level in both genes is different under normal and stress conditions. The results obtained by anti-mazF peptide nucleic acids demonstrated that the expression level of virulence genes had decreased. These findings demonstrate an association between TA systems and virulence factors. The mazEF on the plasmids and the higBA TA genes on the chromosomes of all E. faecium and E. faecalis strains were dominant. Additionally, there was a decrease in the expression of virulence genes in the presence of anti-mazF peptide nucleic acids. Therefore, it is suggested that mazEF TA systems are potent and sensitive targets in all E. faecium and E. faecalis strains. PMID:26005332

  3. The ability of AIF-1 to activate human vascular smooth muscle cells is lost by mutations in the EF-hand calcium-binding region

    International Nuclear Information System (INIS)

    Autieri, Michael V.; Chen Xing

    2005-01-01

    Allograft Inflammatory Factor-1 (AIF-1) is a cytoplasmic calcium-binding protein expressed in vascular smooth muscle cells (VSMC) in response to injury or cytokine stimulation. AIF-1 contains a partially conserved EF-hand calcium-binding domain, and participates in VSMC activation by activation of Rac1 and induction of Granulocyte-Colony Stimulating Factor (G-CSF) expression; however, the mechanism whereby AIF-1 mediates these effects is presently uncharacterized. To determine if calcium binding plays a functional role in AIF-1 activity, a single site-specific mutation was made in the EF-hand calcium-binding domain to abrogate binding of calcium (AIF-1ΔA), which was confirmed by calcium overlay. Functionally, similar to wild-type AIF-1, AIF-1ΔA was able to polymerize F-actin in vitro. However, in contrast to wild-type AIF-1, over-expression of AIF-1ΔA was unable to increase migration or proliferation of primary human VSMC. Further, it was unable to activate Rac1, or induce G-CSF expression to the degree as wild-type AIF-1. Taken together, modification of the wild-type EF-hand domain and native calcium-binding activity results in a loss of AIF-1 function. We conclude that appropriate calcium-binding potential is critical in AIF-1-mediated effects on VSMC pathophysiology, and that AIF-1 activity is mediated by Rac1 activation and G-CSF expression

  4. Exploring the Strengths and Limits of Strong and Weak Sustainability Indicators: A Case Study of the Assessment of China’s Megacities with EF and GPI

    Directory of Open Access Journals (Sweden)

    Lu Huang

    2018-01-01

    Full Text Available The perspective of strong/weak sustainability has a great impact on sustainability assessment. In this study, two most widely used indices, Ecological Footprint (EF and Genuine Progress Indicator (GPI for strong and weak sustainability assessment, were employed to evaluate the sustainability of China’s ten megacities between 1978 and 2015. The results showed that the ecological footprint had been enlarged in the past twenty years; while the genuine economic welfare started to increase since 2005. The cities of Xi’an, Chengdu, Chongqing, and Shanghai met the threshold of below 2.5 global hectares for EF/capita, and over 3000 dollars/capita (in 2010 US$ for GPI/capita. By analyzing and comparing the characteristics, the processes and results, and the complementary features of evaluation methods of EF and GPI, the research suggested that: (1 Strong and weak sustainability indicators, with their own pros/cons in sustainability assessment, should be used carefully; (2 Weak sustainability indicators could be analyzed from the perspective of strong sustainability; (3 Strong sustainability indicators need to be developed urgently. The results in this study could guide the selection of sustainability indicators, and help interpret the results of sustainability assessment.

  5. Um estudo sobre a viabilidade de implantação do pregão eletrônico e uma contribuição na apuração dos resultados nos processos licitatórios

    Directory of Open Access Journals (Sweden)

    Wellinghton de Souza

    2008-08-01

    Full Text Available Este artigo tem por objetivo identificar, com base em um estudo exploratório/descritivo e análise qualitativa/quantitativa, após a implantação do pregão eletrônico, o impacto nas licitações das empresas/entidades no Estado do Espírito Santo. Apresentam-se na revisão da literatura as principais notícias divulgadas pelas empresas/entidades que já implantaram o pregão eletrônico. São realizadas análises das entrevistas com utilização de formulário em 25 das 39 empresas/entidades no Estado do Espírito Santo. Conclui-se que: a o pregão eletrônico reduz os preços unitários dos produtos/serviços nas licitações por permitir maior quantidade de participantes; b o pregão eletrônico reduz os custos operacionais nas licitações por reduzir os custos com impressão de editais e cópias tendo pouca referência à mão-de-obra; c a dificuldade encontrada com maior relevância é o desconhecimento do sistema, sendo superado por meio da intensificação nos treinamentos; d a vantagem com melhor performance é a maior concorrência dos licitantes, sendo a principal desvantagem a queda na conexão, entretanto, o consenso apontado é que houve melhora quanto à transparência do processo com o fim das cartas marcadas entre os fornecedores. Sugere-se nova forma de apuração dos resultados, sendo restrito à população analisada. Não foi objetivo do trabalho saber se a corrupção aumentou ou reduziu após a implantação do Pregão Eletrônico devido à elevada complexidade de tal assunto.

  6. Reducing consumption through communal living

    Energy Technology Data Exchange (ETDEWEB)

    Herring, Horace [The Open Univ., Milton Keynes (United Kingdom). Energy and Environment Research Unit

    2003-07-01

    This paper examines ways consumers and communities can voluntarily adopt a low consumption (or low carbon) lifestyle, often termed 'voluntary simplicity' or a policy of 'sufficiency'. There is an increasing academic literature within Europe in the last five years on the whole question of 'sustainable consumption', and the relationship between income levels and consumption particularly at the household. This debate has moved beyond 'green consumerism' to look at building 'new concepts of prosperity' through local community actions, or reducing working time to allow more time for the creation of social capital. The paper will concentrate on one aspect of the quest for sustainable communities, the relevance of communal living to reducing consumption through examining energy consumption (both direct and indirect) in one such community in the UK. The results from this preliminary study reveal that it is not the sharing of resources that reduces consumption but the mutual reinforcement of attitudes towards a low consumption lifestyle. Thus it is the creation of social capital in a community that is its key to its ecological lifestyle.

  7. Survey on marine food consumption

    International Nuclear Information System (INIS)

    Anon.

    1974-01-01

    Predicting the future effluence of low level radioactive waste water from the nuclear fuel retreating facilities to the ocean, critical food and critical group were investigated in the inhabitants of the coast of Ibaragi Prefecture since 1969. The survey included investigation of drinking water, menu of meal, and marine food consumption, and the results of the third item were chiefly presented in this paper. Both interview by visiting each family, and questionaire were adopted for investigation. Subjects were fishermans' families in Wada-cho in Chiba Prefecture and Kuji-cho in Hitachi City, non-fishermans' families in Tokai vilage, and both families in Nakaminato City and Oarai. The ratio of animal protein consumption per whole protein consumption was remarkably higher than the average of all over the country(23.8 per cent), showing 49 per cent in Kuji-cho. Fishermans' families in Kuji-cho revealed to be a critical group. Marine products of their whole body edible included immature anchovy, sardine, and immature prawn with their maximum individual consumption being 5 kg, 10 kg, and 5.6 kg respectively. Therefore, sardine and immature prawn should be taken care of other than immature anchovy. Marine food consumption of a person per day was estimated from the amount consumed during one week in every season, i.e., during 28 days a year. Marine food consumption of fishermans' families in Kuji-cho showed no seasonal change. Average of marine food consumption in fishermans' families of Kuji-cho and Nakaminato, was 190 g and 132 g of raw fishes, 8 g and 6 g of raw shells, and 4 g and 5 g of dried algae. Consumption frequency and consumption rate of marine foods by kinds and seasons were presented in the tables. (Mukohata, S.)

  8. Muslim consumption and anti-consumption in Malaysia

    DEFF Research Database (Denmark)

    Fischer, Johan

    2015-01-01

    in Malaysia became the subject of increasing consumer activism and I explore how Malaysian federal state institutions, Islamic organizations and consumers respond to and are affected by calls to boycott (anti-consumption) and boycott (consumption) a range of products. More specifically, this article examines...... the above issues building on ethnography from fieldwork with Muslim Consumers Association of Malaysia (PPIM), which is an organization that protects the interests of Muslim consumers and entrepreneurs, as well as Malay Muslim middle-class informants....

  9. The intersubjectivity of family consumption

    DEFF Research Database (Denmark)

    Linnet, Jeppe Trolle

    identity and family scape, I analyze how family members negotiate purchase decisions, and relate to each other’s preferences and desires, as well as those of other families. I consider how family members' interrelations as consumers and long-term negotiations over symbolic meanings of consumption......This study of everyday consumption is based on an ethnographic fieldwork conducted among four Danish middle-class families in Copenhagen, from which an illustrative example of family car purchase is drawn. By introducing two new concepts to the study of family consumption; intra-family consumer...

  10. Arte y arquitectura efímera en los funerales reales de Córdoba del Tucumán

    Directory of Open Access Journals (Sweden)

    Page, Carlos A.

    2009-12-01

    gráficas de túmulos como los de Lima o México, pero sí una serie de documentos que describen estos monumentos que fueron el mejor exponente de la arquitectura efímera de su tiempo. Son testimonios que reunieron sistemas de representación que permitieron exponer además de la arquitectura, la escultura, pintura y literatura que situaban al espectador frente a todas las expresiones artísticas que pudieran trasmitir el doloroso mensaje.

  11. Viability, biofilm formation, and MazEF expression in drug-sensitive and drug-resistant Mycobacterium tuberculosis strains circulating in Xinjiang, China.

    Science.gov (United States)

    Zhao, Ji-Li; Liu, Wei; Xie, Wan-Ying; Cao, Xu-Dong; Yuan, Li

    2018-01-01

    Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) is one of the most common chronic infectious amphixenotic diseases worldwide. Prevention and control of TB are greatly difficult, due to the increase in drug-resistant TB, particularly multidrug-resistant TB. We speculated that there were some differences between drug-sensitive and drug-resistant MTB strains and that mazEF 3,6,9 toxin-antitoxin systems (TASs) were involved in MTB viability. This study aimed to investigate differences in viability, biofilm formation, and MazEF expression between drug-sensitive and drug-resistant MTB strains circulating in Xinjiang, China, and whether mazEF 3,6,9 TASs contribute to MTB viability under stress conditions. Growth profiles and biofilm-formation abilities of drug-sensitive, drug-resistant MTB strains and the control strain H37Rv were monitored. Using molecular biology experiments, the mRNA expression of the mazF 3, 6, and 9 toxin genes, the mazE 3, 6, and 9 antitoxin genes, and expression of the MazF9 protein were detected in the different MTB strains, H37RvΔ mazEF 3,6,9 mutants from the H37Rv parent strain were generated, and mutant viability was tested. Ex vivo culture analyses demonstrated that drug-resistant MTB strains exhibit higher survival rates than drug-sensitive strains and the control strain H37Rv. However, there was no statistical difference in biofilm-formation ability in the drug-sensitive, drug-resistant, and H37Rv strains. mazE 3,6 mRNA-expression levels were relatively reduced in the drug-sensitive and drug-resistant strains compared to H37Rv. Conversely, mazE 3,9 expression was increased in drug-sensitive strains compared to drug-resistant strains. Furthermore, compared with the H37Rv strain, mazF 3,6 expression was increased in drug-resistant strains, mazF 9 expression was increased in drug-sensitive strains, and mazF 9 exhibited reduced expression in drug-resistant strains compared with drug-sensitive strains. Protein expression of mazF9

  12. 1999 world energy consumption (ENERDATA)

    International Nuclear Information System (INIS)

    Martin, J.M.

    2000-01-01

    Here is given a compilation of detailed statistical tables on various aspects of world energy production and consumption over the years 1994 to 1999. The present tables indicate the production, trade and consumption of crude oil, liquefied natural gas, oil products, natural gas, coal, lignite, electric power; the energy balance for the year 1999; the total energy consumption in European Union, Western Europe, North America, Japan and Pacific, CIS and Central Europe, Latin America, Asia, Middle East and Africa for the years 1994 to 1999. The CO 2 emissions for these countries are also given. These data are an extraction of the energy statistics yearbook, ENERDATA, June 2000. They are commented by Mr J.M. Martin. According to ENERDATA, the 1999 world energy consumption stagnates. (O.M.)

  13. Inconsistent Investment and Consumption Problems

    DEFF Research Database (Denmark)

    Kronborg, Morten Tolver; Steffensen, Mogens

    2015-01-01

    In a traditional Black–Scholes market we develop a verification theorem for a general class of investment and consumption problems where the standard dynamic programming principle does not hold. The theorem is an extension of the standard Hamilton–Jacobi–Bellman equation in the form of a system...... of non-linear differential equations. We derive the optimal investment and consumption strategy for a mean-variance investor without pre-commitment endowed with labor income. In the case of constant risk aversion it turns out that the optimal amount of money to invest in stocks is independent of wealth...... of future labor income net of consumption. Using the verification theorem we give a detailed analysis of this problem. It turns out that the optimal amount of money to invest in stocks is given by a linear function of wealth plus the financial value of future labor income net of consumption. The optimal...

  14. Heavier smoking increases coffee consumption

    DEFF Research Database (Denmark)

    Bjørngaard, Johan H; Nordestgaard, Ask Tybjærg; Taylor, Amy E

    2017-01-01

    Background: There is evidence for a positive relationship between cigarette and coffee consumption in smokers. Cigarette smoke increases metabolism of caffeine, so this may represent a causal effect of smoking on caffeine intake. Methods: We performed Mendelian randomization analyses in the UK...... Biobank ( N  = 114 029), the Norwegian HUNT study ( N  = 56 664) and the Copenhagen General Population Study (CGPS) ( N  = 78 650). We used the rs16969968 genetic variant as a proxy for smoking heaviness in all studies and rs4410790 and rs2472297 as proxies for coffee consumption in UK Biobank and CGPS....... Analyses were conducted using linear regression and meta-analysed across studies. Results: Each additional cigarette per day consumed by current smokers was associated with higher coffee consumption (0.10 cups per day, 95% CI: 0.03, 0.17). There was weak evidence for an increase in tea consumption per...

  15. Manufacturing consumption of energy 1991

    Energy Technology Data Exchange (ETDEWEB)

    1994-12-01

    This report provides estimates on energy consumption in the manufacturing sector of the US economy. These estimates are based on data from the 1991 Manufacturing Energy Consumption Survey (MECS). This survey--administered by the Energy End Use and Integrated Statistics Division, Office of Energy Markets and End Use, Energy Information Administration (EIA)--is the most comprehensive source of national-level data on energy-related information for the manufacturing industries.

  16. CREATING VALUE WITHIN CONSUMPTION CULTURE

    Directory of Open Access Journals (Sweden)

    Daniel Adrian Gârdan

    2016-12-01

    Full Text Available The issue of culture consumption is a particular concern within the modern marketing theory. Culture can be seen as representing a body of knowledge, beliefs, values, attitudes, symbols etc, developed in a certain period of time by a group of individuals, items transmitted with the help of a social learning process to other generations within the group. Thus, the consumption of culture will identify itself with the consumption of any product, service or a combination of them, directly resulted as manifestation of culture, expressions of artistic creativity specific for a certain cultural space. The present paper proposes the analysis of the phenomenon referring to the culture consumption in terms of specific characteristics. The paper reviews the features specific to the modern consumer of culture, the relationship that exists between the individuals’ level of education and the culture consumption and value creation process or augmentation of the intrinsic value of an artistic product as a result of the contribution that the culture consumer can bring himself. The authors highlight the fact that within extremely complex processes which are defining the culture consumption, consumers can assume an active role, becoming on their turn co-participants in the cultural goods and services value creation and transmission. The modern consumer benefits more than ever from the advantages offered by the information technology, being called to respond to major challenges of the postmodernism paradigm in terms of culture consumption. Globalization and other social economic and politic phenomena have profoundly changed the reports between individual and culture, between self and other members of the society, causing synthesis and essential transformations of culture consumption, of culture consumers typologies, and not least of the very forms of artistic expression related to cultural goods and services.

  17. Price and consumption of tobacco

    Directory of Open Access Journals (Sweden)

    Virendra Singh

    2012-01-01

    Full Text Available Background: It is thought that price increase in tobacco products leads to reduced consumption. Though many studies have substantiated this concept, it has not been well studied in India. Recently, price of tobacco products was increased due to ban on plastic sachets of chewing tobacco and increased tax in Rajasthan. This study was designed to evaluate the effect of price rise on overall consumption of tobacco in Jaipur city, Rajasthan. Materials and Methods: This study was carried out in Jaipur city. Two-staged stratified sampling was used. In the first phase of study, cost and consumption of various tobacco products in the months of February and April were enquired from 25 retail tobacco shops. In the second phase, tobacco consumption was enquired from 20 consecutive consumers purchasing any tobacco product from all the above retail tobacco shops. The data were statistically analyzed using descriptive statistics and paired "t" test. Results: The comparison of prices of tobacco products between February and April revealed that the price of cigarette, bidi, and chewing tobacco has increased by 19%, 21%, and 68%, respectively. Average decrease in sales of cigarettes, bidi, and chewing tobacco at shops included in the study were 14%, 23%, and 38%, respectively. The consumers purchasing tobacco also reported decreased consumption. Chewing tobacco showed the maximum reduction (21%. Consumption of cigarette and bidi has also reduced by 15% and 13%, respectively. Conclusion: It may be concluded that reduction in consumption is associated with increased price of tobacco products. Reduced consumption is comparative to the magnitude of price increase.

  18. Does fertility decrease household consumption?

    OpenAIRE

    Jungho Kim; Henriette Engelhardt; Alexia Fürnkranz-Prskawetz; Arnstein Aassve

    2009-01-01

    This paper presents an empirical analysis of the relationship between fertility and a direct measure of poverty for Indonesia, a country, which has experienced unprecedented economic growth and sharp fertility declines over recent decades. It focuses on illustrating the sensitivity of the effect of fertility on household consumption with respect to the equivalence scale by applying the propensity score matching method. The analysis suggests that a newborn child decreases household consumption...

  19. Consumption Profiles for Future Households

    OpenAIRE

    Blikø, Marianne

    2015-01-01

    Over the last few years there has been a change in energy consumption in Norwegian households. New houses are far better insulated, have high demands for efficient energy carriers and new installations that affect the electricity consumption. Today, most of the electricity in a Norwegian household is used for space heating, but this demand is expected to be reduced in the future, mainly because the need for space heating is reduced as a result of stricter demands for isolation. Electricity co...

  20. Determinants of Organic Products Consumption

    Directory of Open Access Journals (Sweden)

    Matheus Wemerson Gomes Pereira

    2015-04-01

    Full Text Available The increased environmental awareness and the growing desire for safer foods to health made the organic products category one of the most growing in the food sector. Thus, this study aimed to identify the most significant socioeconomic variables that influence the frequency of organic products consumption, and for that, it was based on a quantitative-descriptive study with a probability sample of 400 individuals, residing of the urban area of Campo Grande/MS, Brazil, responsible, alone or not, for food purchasing in family. As a tool for data collection, was used a structured questionnaire developed from socioeconomic variables and frequency of consumption of organic products. For the data analysis was performed statistical/econometric tests with the STATA 11.0 statistical software, using Multinomial Logit model (MNL, addition to verification of the explanatory variables effect on the probability of the consumption frequency levels of organic products through the Relative Risk Ratio (RRR, and was analyzed the Marginal Effect (ME exercised by the explanatory variables in each frequency level of organic products consumption. The results showed that the explanatory variables gender, education and economic class were statistically significant in the probability of an individual belong to some of the levels of consumption (rare, occasional and frequent over never having consumed organic products, being higher frequency of consumption when the consumer is female, have higher education or is of higher economic class.

  1. Turismo e Hospitalidade no cenário da comunicação científica: avaliação de periódicos científicos eletrônicos

    Directory of Open Access Journals (Sweden)

    Elaine Cristina Pinto de Miranda

    2013-12-01

    Full Text Available Com o avanço da tecnologia da informação, os periódicos científicos estão sendo publicados cada vez mais em versão eletrônica na internet, o que agiliza a circulação, acesso e uso dos seus conteúdos. Este artigo avalia a qualidade de 19 periódicos eletrônicos em Turismo e Hospitalidade publicados no Brasil, que se encontravam ativos em 2011. Verificou-se que a maioria dos periódicos tinha periodicidade semestral e era publicado por universidades da região Sudeste do País. Notou-se o crescimento de novos títulos, especialmente a partir de meados da década de 2000, associado ao uso do software SEER e à inserção no movimento do acesso aberto, o que trouxe benefícios para a área. A avaliação de um conjunto de 74 indicadores de padrões de normalização nacionais e internacionais indicou o desempenho desses veículos, variando entre 40 e 72 pontos, com destaque para quatro deles: Caderno Virtual de Turismo, Revista Brasileira de Pesquisa em Turismo, Turismo em Análise, Turismo - Visão e Ação. Constatou-se que nenhum periódico alcançou a pontuação máxima de 90 pontos, e foram apontados os principais indicadores não atendidos. Há necessidade de novos estudos e ações junto a editores e autores, além de apoio de instituições de fomento. Tourism and Hospitality in the Scenario of Scientific Communication: Analysis of Electronic Scientific Journals - With the advancement of information technology, scientific journals are being increasingly published in the electronic version on the Internet, which speeds up the circulation, access and use of its contents. This article evaluates the quality of 19 Tourism and Hospitality journals published in Brazil and that were active in 2011. It was found that most journals had biannually publication and was published by universities in the Southeast of the country. It was noted the growth of new titles, especially from the mid-2000s, associated with the use of the software SEER

  2. Design and implementation of an electronic architecture for an agricultural mobile robot Projeto e implementação de uma arquitetura eletrônica para um robô agrícola móvel

    Directory of Open Access Journals (Sweden)

    Eduardo P. Godoy

    2010-11-01

    Full Text Available A current trend in the agricultural area is the development of mobile robots and autonomous vehicles for remote sensing. One of the major challenges in the design of these robots is the development of the electronic architecture for the integration and control of the devices. Recent applications of mobile robots have used distributed architectures based on communication networks. A technology that has been widely used as an embedded network is the CAN protocol. The implementation of the ISO11783 standard represents the standardization of the CAN for application in agricultural machinery. This work describes the design and implementation of an electronic architecture for a mobile agricultural robot. The discussions are focused on the developed architecture, the wireless communication system for teleoperation and the distributed control based on CAN protocol and ISO11783. The evaluation of the developed system was based on the analysis of performance parameters such as motor response and architectural time delay obtained with the robot operation. The results show that the developed architecture can be applied for teleoperation and distributed control of agricultural robots meeting the requirements for accurate robot movement and an acceptable response time for robot control commands and supervision.Uma tendência atual na área agrícola é o desenvolvimento de robôs móveis e veículos autônomos para sensoriamento remoto. Um dos grandes desafios no projeto desses robôs é o desenvolvimento da arquitetura eletrônica para integração e controle dos dispositivos. Em aplicações recentes desses robôs tem-se utilizado arquiteturas distribuídas baseadas em redes de comunicação. Uma tecnologia amplamente usada como rede embarcada é o protocolo CAN. A implementação da norma ISO11783 representa a padronização do protocolo CAN para aplicações agrícolas. Este artigo apresenta o projeto e a implementação de uma arquitetura eletr

  3. Desenvolvimento e calibração de um tensiômetro eletrônico de leitura automática Development and calibration of an electronic tensiometer for automatic reading

    Directory of Open Access Journals (Sweden)

    Adunias S. Teixeira

    2005-08-01

    Full Text Available O presente trabalho apresenta o desenvolvimento e calibração de tensiômetro de leitura automática, sendo o tensiômetro de mercúrio utilizado como padrão de comparação. Os ensaios foram conduzidos no Laboratório de Mecânica e Eletrônica do Departamento de Engenharia Agrícola da UFC. Esse equipamento difere do tensiômetro tradicional por substituir o manômetro de mercúrio por sensor de pressão. Tal dispositivo gera uma saída com valor de até no máximo 4,5 volts. O tensiômetro eletrônico é conectado a uma placa de aquisição de dados (DAQ contendo um microprocessador, conversor analógico/digital (ADC e saída serial, sendo tal placa ligada a um microcomputador. A bancada de ensaio constituiu-se de três caixas plásticas preenchidas com solo de textura franco- arenosa. Os dados foram coletados durante um mês e as equações geradas foram do tipo Count = offset + apsim, em que psim representa o potencial matricial fornecido pelo tensiômetro de mercúrio (kPa e count a saída digial do ADC, com valores adimensionais de 0 a 4.095. Foram obtidos os valores máximo e mínimo de offset de 348,572 e 261,026, e de coeficiente angular de 36,675 kPa-1 e 34,421 kPa-1. Os resultados da regressão linear simples indicaram a existência de regressão a menos de 0,1% de significância e valores de coeficientes de correlação nunca inferiores a 0,9994.This paper presents the development and calibration of a tensiometer for automatic reading where mercury has been replaced by an electronic pressure sensor called the electronic tensiometer. The conventional mercury tensiometer was used as a standard for calibration. Calibration trials were conducted at the Mechanics and Electronics Laboratory of the Department of Agricultural Engineering at Ceará Federal University, Brazil. The electronic tensiometer outputs a maximum 4.5 volts and is connected to a data acquisition system (DAQ composed of a microprocessor, a 12-bit analog to digital

  4. The industrial energy consumption in 1999

    International Nuclear Information System (INIS)

    Anon.

    2001-01-01

    The Danish industrial energy consumption in 1999 is presented in tables. The tables include: the development in the energy consumption, the amount of employees in each of the main branches, fuel consumption, the fuel and energy consumption in 1999 based on each group of branches and energy category, the energy consumption in 1997 for each group of branches and the percentage distribution on energy category, and the fuel and energy consumption of motor vehicles in 1999 based on each group of branches. (SM)

  5. A scanning electron microscopic evaluation of different root canal irrigation regimens Avaliação por microscopia eletrônica de varredura de diferentes regimes irrigantes no canal radicular

    Directory of Open Access Journals (Sweden)

    Chaves Medici Mônika

    2006-09-01

    Full Text Available The purpose of this study was to assess the effectiveness of endodontic irrigants in removing the smear layer from instrumented root canal walls using Scanning Electron Microscopy (SEM. The endodontic irrigants used were: 1% sodium hypochlorite (NaOCl; 1% NaOCl mixed to 17% EDTAC; 2% chlorhexidine gel; and Ricinus communis gel. Photomicrographs of the middle and apical thirds were evaluated with the aid of the Fotoscore - v. 2.0 software. The results indicated that the mixture of sodium hypochlorite and EDTAC completely removed the smear layer from dentinal walls. The other endodontic irrigants were not as efficient in cleansing the root canals.A proposta deste estudo foi avaliar, por meio de microscopia eletrônica de varredura (MEV, a efetividade dos irrigantes endodônticos na remoção da "smear layer" das paredes dos canais radiculares instrumentados. Os irrigantes endodônticos utilizados foram: solução de hipoclorito de sódio a 1%; solução de hipoclorito de sódio a 1% misturado ao EDTAC a 17%, gel de clorexidina a 2% e gel de Ricinus communis. Fotomicrografias dos terços médio e apical foram avaliadas com o auxílio do software Fotoscore - versão 2.0. Os resultados indicaram que a mistura da solução de hipoclorito de sódio e EDTAC removeu eficientemente a "smear layer" das paredes dentinárias. Os demais irrigantes endodônticos não foram tão eficientes na limpeza dos canais.

  6. Marketing On-Line, Comércio Eletrónico e Hotelaria: Estudo sobre a Marcação de Hotéis na Internet em Mercados Business-to-Business

    Directory of Open Access Journals (Sweden)

    Jorge Remondes

    2015-08-01

    Full Text Available O marketing digital, o comércio eletrónico e os métodos usados por empresas para marcação de hotéis em viagens de negócios são temas de reflexão na literatura, no senso comum e em alguns estudos empíricos. Este artigo é também um contributo teórico e concetual sobre esta problemática e simultaneamente um contributo para as empresas e gestores pelo facto de dar a conhecer os resultados de um estudo online, de tipo quantitativo, baseado numa amostra de 206 quadros empresariais portugueses que responderam a um questionário nas redes sociais facebook, Linkedin e Twitter, de vinte a vinte e sete de maio de 2013. O objetivo da pesquisa foi analisar comparativamente os métodos de marcação de hotéis em business-to-business (B2B com o intuito de medir a importância da internet nestes mercados em Portugal. Recorreu-se à análise descritiva e aplicação de testes estatísticos para facilitar a interpretação dos dados recolhidos. Os resultados mostram que a proporção de marcação de hotéis, em viagens de negócios, preferencialmente através da internet é a mais elevada das opções possíveis. Por outro lado, antes de efetuarem uma compra recorrem com mais frequência a motores de busca e sites de hotéis. No entanto, os resultados  não confirmam que a internet seja o método preferencial em mercados B2B.

  7. Energy consumption and energy prices

    International Nuclear Information System (INIS)

    Bentzen, J.

    1993-01-01

    Data are presented on energy consumption and energy prices related to a number of OECD (Organisation for Economic Co-operation and Development) lands covering the period 1951-1990. The information sources are described and the development of energy consumption and prices in Denmark are illustrated in relation to these other countries. The energy intensity (the relation between energy consumption and the gross national product) is dealt with. Here it is possible to follow development during the whole post-war period. It is generally understood that Denmark saved large amounts of energy after 1973-74 but, taken over the whole post-war period, savings and decline in energy-gross national product relations are less dramatic compared to conditions in other OECD countries. Energy coefficients or elasticities show the relative rise in consumption compared to the relative rise in gross national product (growth rate). This is shown to be typically unstable and an eventual connection with the amount of energy price increase and/or the growth rate of the national economy is considered. Results of Granger causuality tests on energy consumption, national income and energy prices are presented. Effective energy prices were very low in Denmark up to 1970 when they suddenly began to increase. Since the oil crisis Denmark's energy consumption has fallen whereas the other countries have used rather more energy than before. Effective promotion of energy savings must be seen in relation to the fact that the 1970 basis level of energy consumption and intensity was unusually high. The high effective energy prices have also encouraged energy savings in Denmark. (AB)

  8. [Food consumption patterns among adolescents].

    Science.gov (United States)

    Palenzuela Paniagua, S M; Pérez Milena, A; Pérula de Torres, L A; Fernández García, J A; Maldonado Alconada, J

    2014-01-01

    Adolescence is a critical time for the establishment of healthy eating habits. The objective was to analyze food consumption patterns among adolescents and their relationship with family and social factors. Multicentre observational cross-sectional descriptive study using a food frequency questionnaire for the last week. It was answered anonymously. The adolescent's age/gender, parents' studies/occupation and school's location/type were included. The population sample was composed of 1,095 adolescents in sixth grade at primary schools from an Andalusian region. They were chosen by polietapic random sampling that distinguished between public/private and capital/provincial schools. 1,005 surveys were analyzed. The mean age is 11.45 (SD: 0.59). Fifty-three percent were male. The intake of dairy products (only two-thirds taken daily), pasta, fruit and vegetables (daily consumption of 30%) is deficient. Sixty-four point five percent consume legumes weekly. Fish consumption is equal to meat, with a preference for poultry. More than half consume red meat daily. Olive oil is preferred. The intake of "empty calories" (fast food, candies, soft drink) is high. Through multivariate analysis the existence of clusters of healthy and unhealthy foods, related to the social status of the parents and the type of school, is proved. A healthy diet based on the nutritional pyramid is not the consumption pattern in the adolescents surveyed. There is a low consumption of diary products, legumes, fruits and vegetables. There is a relationship between the social class of the family and consumption patterns (healthy and unhealthy). Health strategies are needed to modify such inappropriate consumption.

  9. Inconsistent Investment and Consumption Problems

    Energy Technology Data Exchange (ETDEWEB)

    Kronborg, Morten Tolver, E-mail: mtk@atp.dk [ATP (Danish Labour Market Supplementary Pension Scheme) (Denmark); Steffensen, Mogens, E-mail: mogens@math.ku.dk [University of Copenhagen, Department of Mathematical Sciences (Denmark)

    2015-06-15

    In a traditional Black–Scholes market we develop a verification theorem for a general class of investment and consumption problems where the standard dynamic programming principle does not hold. The theorem is an extension of the standard Hamilton–Jacobi–Bellman equation in the form of a system of non-linear differential equations. We derive the optimal investment and consumption strategy for a mean-variance investor without pre-commitment endowed with labor income. In the case of constant risk aversion it turns out that the optimal amount of money to invest in stocks is independent of wealth. The optimal consumption strategy is given as a deterministic bang-bang strategy. In order to have a more realistic model we allow the risk aversion to be time and state dependent. Of special interest is the case were the risk aversion is inversely proportional to present wealth plus the financial value of future labor income net of consumption. Using the verification theorem we give a detailed analysis of this problem. It turns out that the optimal amount of money to invest in stocks is given by a linear function of wealth plus the financial value of future labor income net of consumption. The optimal consumption strategy is again given as a deterministic bang-bang strategy. We also calculate, for a general time and state dependent risk aversion function, the optimal investment and consumption strategy for a mean-standard deviation investor without pre-commitment. In that case, it turns out that it is optimal to take no risk at all.

  10. Inconsistent Investment and Consumption Problems

    International Nuclear Information System (INIS)

    Kronborg, Morten Tolver; Steffensen, Mogens

    2015-01-01

    In a traditional Black–Scholes market we develop a verification theorem for a general class of investment and consumption problems where the standard dynamic programming principle does not hold. The theorem is an extension of the standard Hamilton–Jacobi–Bellman equation in the form of a system of non-linear differential equations. We derive the optimal investment and consumption strategy for a mean-variance investor without pre-commitment endowed with labor income. In the case of constant risk aversion it turns out that the optimal amount of money to invest in stocks is independent of wealth. The optimal consumption strategy is given as a deterministic bang-bang strategy. In order to have a more realistic model we allow the risk aversion to be time and state dependent. Of special interest is the case were the risk aversion is inversely proportional to present wealth plus the financial value of future labor income net of consumption. Using the verification theorem we give a detailed analysis of this problem. It turns out that the optimal amount of money to invest in stocks is given by a linear function of wealth plus the financial value of future labor income net of consumption. The optimal consumption strategy is again given as a deterministic bang-bang strategy. We also calculate, for a general time and state dependent risk aversion function, the optimal investment and consumption strategy for a mean-standard deviation investor without pre-commitment. In that case, it turns out that it is optimal to take no risk at all

  11. Perfluoroalkyl substances and fish consumption.

    Science.gov (United States)

    Christensen, Krista Y; Raymond, Michelle; Blackowicz, Michael; Liu, Yangyang; Thompson, Brooke A; Anderson, Henry A; Turyk, Mary

    2017-04-01

    Perfluoroalkyl substances (PFAS) are an emerging class of contaminants. Certain PFAS are regulated or voluntarily limited due to concern about environmental persistence and adverse health effects, including thyroid disease and dyslipidemia. The major source of PFAS exposure in the general population is thought to be consumption of seafood. In this analysis we examine PFAS levels and their determinants, as well as associations between PFAS levels and self-reported fish and shellfish consumption, using a representative sample of the U.S. Data on PFAS levels and self-reported fish consumption over the past 30 days were collected from the 2007-2008, 2009-2010, 2011-2012, and 2013-2014 cycles of the National Health and Nutrition Examination Survey. Twelve different PFAS were measured in serum samples from participants. Ordinary least squares regression models were used to identify factors (demographic characteristics and fish consumption habits) associated with serum PFAS concentrations. Additional models were further adjusted for other potential exposures including military service and consumption of ready-to-eat and fast foods. Seven PFAS were detected in at least 30% of participants and were examined in subsequent analyses (PFDA, PFOA, PFOS, PFHxS, MPAH, PFNA, PFUA). The PFAS with the highest concentrations were PFOS, followed by PFOA, PFHxS and PFNA (medians of 8.3, 2.7, 1.5 and 1.0ng/mL). Fish consumption was generally low, with a median of 1.2 fish meals and 0.14 shellfish meals, reported over the past 30 days. After adjusting for demographic characteristics, total fish consumption was associated with reduced MPAH, and with elevated PFDE, PFNA and PFuDA. Shellfish consumption was associated with elevations of all PFAS examined except MPAH. Certain specific fish and shellfish types were also associated with specific PFAS. Adjustment for additional exposure variables resulted in little to no change in effect estimates for seafood variables. PFAS are emerging

  12. Prediction of natural gas consumption

    International Nuclear Information System (INIS)

    Zhang, R.L.; Walton, D.J.; Hoskins, W.D.

    1993-01-01

    Distributors of natural gas need to predict future consumption in order to purchase a sufficient supply on contract. Distributors that offer their customers equal payment plans need to predict the consumption of each customer 12 months in advance. Estimates of previous consumption are often used for months when meters are inaccessible, or bimonthly-read meters. Existing methods of predicting natural gas consumption, and a proposed new method for each local region are discussed. The proposed model distinguishes the consumption load factors from summer to other seasons by attempting to adjust them by introducing two parameters. The problem is then reduced to a quadratic programming problem. However, since it is not necessary to use both parameters simultaneously, the problem can be solved with a simple iterative procedure. Results show that the new model can improve the two-equation model to a certain scale. The adjustment to heat load factor can reduce the error of prediction markedly while that to base load factor influences the error marginally. 3 refs., 11 figs., 2 tabs

  13. Children's media consumption in Serbia

    Directory of Open Access Journals (Sweden)

    Filipović Jelena

    2009-01-01

    Full Text Available The paper aims at providing an insight into media consumption among children in Serbia. A consumer survey was conducted to examine differences in children's consumption of various advertising media based on their age, gender and hometown. The research findings revealed significant differences between the two age cohorts in the three out of four tested media categories (TV, print, radio and Internet. On the other hand, the research findings also revealed variations in consumption of radio and print media, and no difference in the consumption of television and Internet between boys and girls. Finally, the research findings suggested significant differences in the consumption of Internet among children living in five cities in Serbia. Though the sample included more than 700 children it cannot be considered strictly representative for the whole country since the survey was conducted in urban areas only. However, the greatest value of this study comes from the fact that the research was placed in Serbia as a transitional society of the Western Balkans which has been seriously underrepresented in the main body of contemporary marketing theory and research studies. Therefore, one of the main goals of this paper was to make a contribution to better understanding of local conditions that might influence marketing decisions when communicating with children as a specific target group on Serbian market.

  14. Meat Consumption Culture in Ethiopia

    Science.gov (United States)

    Jo, Cheorun

    2014-01-01

    The consumption of animal flesh food in Ethiopia has associated with cultural practices. Meat plays pivotal and vital parts in special occasions and its cultural symbolic weight is markedly greater than that accorded to most other food. Processing and cooking of poultry is a gender based duty and has socio-cultural roles. Ethiopians are dependent on limited types of animals for meats due to the taboo associated culturally. Moreover, the consumption of meat and meat products has a very tidy association with religious beliefs, and are influenced by religions. The main religions of Ethiopia have their own peculiar doctrines of setting the feeding habits and customs of their followers. They influence meat products consumption through dictating the source animals that should be used or not be used for food, and scheduling the days of the years in periodical permeation and restriction of consumptions which in turn influences the pattern of meat consumption in the country. In Ethiopia, a cow or an ox is commonly butchered for the sole purpose of selling within the community. In special occasions, people have a cultural ceremony of slaughtering cow or ox and sharing among the group, called Kircha, which is a very common option of the people in rural area where access of meat is challenging frequently. PMID:26760739

  15. Effectiveness of hCMV, mEF1a and mAct promoters on driving of foreign gene expression in transgenic zebrafish

    Directory of Open Access Journals (Sweden)

    . Alimuddin

    2007-01-01

    Full Text Available Highly unsaturated fatty acids (HUFA, especially eicosapentaenoic acid (EPA and docosahexaenoic acid (DHA have long been recognized for its beneficial effect for human health and development.   The D6 fatty acid desaturase is generally considered to be the rate-limiting factor in HUFA biosynthesis.  Here, as the first step of study, we conducted experiment to select an appropriate construct that allows higher expression levels of masu salmon (Oncorhynchus masou D6-desaturase gene in zebrafish (Danio rerio in order to increase its activity for synthesizing EPA/DHA.  Salmon D6-desaturase cDNA (sD6 was separately ligated with human cytomegalovirus (hCMV, medaka elongation factor 1a (mEF1a and medaka b-actin (mAct promoters.  The resulted construct was designated as hCMV-sD6, mEF1a-sD6 and mAct-sD6, respectively.  Each of the constructs in circular DNA form was microinjected into 1-cell stage embryos at a concentration of 30mg/ml. Transgenic individuals were identified by polymerase chain reaction (PCR and their expression levels were analyzed by reverse transcription PCR.  The first (F1 and second (F2 generation was produced by crossing the transgenic founder F0 and F1, respectively, with wild-type fish.  The results showed that the highest transient gene expression level was obtained from the mAct-D6 construct, followed respectively by EF1a-D6 and hCMV-D6 construct. The transmission rate of transgene into F1 generation was 4.2%-44.1%, and into F2 was followed the Mendellian segregation pattern.   Expression of transgene in F2 generation was varied between strains regarding as the mosaics of F0 fish.  Now, a transgenic system to study the modification of fatty acid biosynthesis pathways in fish was established.  Further investigations are to produce fish containing higher levels of EPA and DHA. Keywords: desaturase, nutraceutical fatty acid, transgenic, zebrafish, masu salmon   Abstrak Promoter merupakan regulator yang menentukan

  16. Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin.

    Science.gov (United States)

    Liu, Wen; Duan, Lian; Sun, Tijian; Yang, Binsheng

    2016-12-01

    Ciliate Euplotes octocarinatus centrin (EoCen) is an EF-hand calcium-binding protein closely related to the prototypical calcium sensor protein calmodulin. Four mutants (D37K, D73K, D110K and D146K) were created firstly to elucidate the importance of the first aspartic acid residues (Asp37, Asp73, Asp110 and Asp146) in the beginning of the four EF-loops of EoCen. Aromatic-sensitized Tb 3+ fluorescence indicates that the aspartic acid residues are very important for the metal-binding of EoCen, except for Asp73 (in EF-loop II). Resonance light scattering (RLS) measurements for different metal ions (Ca 2+ and Tb 3+ ) binding proteins suggest that the order of four conserved aspartic acid residues for contributing to the self-assembly of EoCen is Asp37 > Asp146 > Asp110 > Asp73. Cross-linking experiment also exhibits that Asp37 and Asp146 play critical role in the self-assembly of EoCen. Asp37, in site I, which is located in the N-terminal domain, plays the most important role in the metal ion-dependent self-assembly of EoCen, and there is cooperativity between N-terminal and C-terminal domain (especially the site IV). In addition, the dependence of Tb 3+ induced self-assembly of EoCen and the mutants on various factors, including ionic strength and pH, were characterized using RLS. Finally, 2-p-toluidinylnaphthalene-6-sulfonate (TNS) binding, ionic strength and pH control experiments indicate that in the process of EoCen self-assembly, molecular interactions are mediated by both electrostatic and hydrophobic forces, and the hydrophobic interaction has the important status.

  17. The Drosophila melanogaster Eip74EF-PA transcription factor directly binds the sciarid BhC4-1 promoter.

    Science.gov (United States)

    Frank, Henrique Oliveira; Sanchez, Danilo Garcia; de Freitas Oliveira, Lucas; Kobarg, Jörg; Monesi, Nadia

    2017-11-01

    The DNA puff BhC4-1 gene of Bradysia hygida (Diptera, Sciaridae) is amplified and expressed in the salivary glands at the end of the last larval instar. Even though there are no BhC4-1 orthologs in Drosophila melanogaster, the mechanisms that regulate BhC4-1 gene expression in B. hygida are for the most part conserved in D. melanogaster. The BhC4-1 promoter contains a 129bp (-186/-58) cis-regulatory module (CRM) that drives developmentally regulated expression in transgenic salivary glands at the onset of metamorphosis. Both in the sciarid and in transgenic D. melanogaster, BhC4-1 gene expression is induced by the increase in ecdysone titers that triggers metamorphosis. Genetic interaction experiments revealed that in the absence of the Eip74EF-PA early gene isoform BhC4-1-lacZ levels of expression in the salivary gland are severely reduced. Here we show that the overexpression of the Eip74EF-PA transcription factor is sufficient to anticipate BhC4-1-lacZ expression in transgenic D. melanogaster. Through yeast one-hybrid assays we confirm that the Eip74EF-PA transcription factor directly binds to the 129 bp sciarid CRM. Together, these results contribute to the characterization of an insect CRM and indicate that the ecdysone gene regulatory network that promotes metamorphosis is conserved between D. melanogaster and the sciarid B. hygida. © 2017 Wiley Periodicals, Inc.

  18. Cigarette, alcohol, and caffeine consumption

    DEFF Research Database (Denmark)

    Rasch, Vibeke

    2003-01-01

    OBJECTIVE: To study the association between cigarette, alcohol, and caffeine consumption and the occurrence of spontaneous abortion. METHODS: The study population consisted of 330 women with spontaneous abortion and 1168 pregnant women receiving antenatal care. A case-control design was utilized;...... units alcohol per week and 375 mg or more caffeine per day during pregnancy may increase the risk of spontaneous abortion.......OBJECTIVE: To study the association between cigarette, alcohol, and caffeine consumption and the occurrence of spontaneous abortion. METHODS: The study population consisted of 330 women with spontaneous abortion and 1168 pregnant women receiving antenatal care. A case-control design was utilized......; cases were defined as women with a spontaneous abortion in gestational week 6-16 and controls as women with a live fetus in gestational week 6-16. The variables studied comprise age, parity, occupational situation, cigarette, alcohol, and caffeine consumption. The association between cigarette, alcohol...

  19. Palatable Food Consumption in Children

    DEFF Research Database (Denmark)

    De Decker, Annelies; Verbeken, Sandra; Sioen, Isabelle

    2017-01-01

    To understand the importance of the home food environment on unhealthy food consumption in children high in reward sensitivity, this study tested the hypothesis that the home availability of unhealthy food moderates the effect of reward sensitivity on children’s fast-food consumption frequency......, exerted via food cue responsiveness. Children between 7.5 and 14 years (n = 174, 50.6% boys) reported on reward sensitivity and food cue responsiveness (by means of the subscale ‘external eating’). Their height and weight were measured. Parents reported on their children’s fast-food consumption frequency......, food cue responsiveness (by means of the subscale ‘food responsiveness’), and on the home availability of unhealthy foods. Two moderated mediation models were conducted, one with the parent- and one with the child-reported food cue responsiveness as mediator. Findings suggested that with a high home...

  20. Moral Violations Reduce Oral Consumption

    Science.gov (United States)

    Chan, Cindy; Van Boven, Leaf; Andrade, Eduardo B.; Ariely, Dan

    2014-01-01

    Consumers frequently encounter moral violations in everyday life. They watch movies and television shows about crime and deception, hear news reports of corporate fraud and tax evasion, and hear gossip about cheaters and thieves. How does exposure to moral violations influence consumption? Because moral violations arouse disgust and because disgust is an evolutionarily important signal of contamination that should provoke a multi-modal response, we hypothesize that moral violations affect a key behavioral response to disgust: reduced oral consumption. In three experiments, compared with those in control conditions, people drank less water and chocolate milk while (a) watching a film portraying the moral violations of incest, (b) writing about moral violations of cheating or theft, and (c) listening to a report about fraud and manipulation. These findings imply that “moral disgust” influences consumption in ways similar to core disgust, and thus provide evidence for the associations between moral violations, emotions, and consumer behavior. PMID:25125931

  1. Risk thresholds for alcohol consumption

    DEFF Research Database (Denmark)

    Wood, Angela M; Kaptoge, Stephen; Butterworth, Adam S

    2018-01-01

    previous cardiovascular disease. METHODS: We did a combined analysis of individual-participant data from three large-scale data sources in 19 high-income countries (the Emerging Risk Factors Collaboration, EPIC-CVD, and the UK Biobank). We characterised dose-response associations and calculated hazard......BACKGROUND: Low-risk limits recommended for alcohol consumption vary substantially across different national guidelines. To define thresholds associated with lowest risk for all-cause mortality and cardiovascular disease, we studied individual-participant data from 599 912 current drinkers without......·4 million person-years of follow-up. For all-cause mortality, we recorded a positive and curvilinear association with the level of alcohol consumption, with the minimum mortality risk around or below 100 g per week. Alcohol consumption was roughly linearly associated with a higher risk of stroke (HR per 100...

  2. Energy consumption trends in Lithuania

    International Nuclear Information System (INIS)

    Galinis, A.; Miskinis, V.

    2000-01-01

    The paper describes some problems related to integration into EU, current state of the Lithuania economy and energy sector and changes in energy consumption during transition period. It provides and analysis of the main indicators of energy consumption, such as the ratio of primary energy consumption to Gross Domestic Product (GDP), primary and final energy intensity and others based on estimates of Purchasing Power Parity. The paper also discusses problems arising at evaluation of economical and energy indices for the countries in transition and compares them with those existing in other countries of Central and Eastern Europe and in Western countries. It shows uneven tendencies of energy intensity occurring under transitions in Lithuania and other Baltic States. (author)

  3. Tattoos, piercings, and alcohol consumption.

    Science.gov (United States)

    Guéguen, Nicolas

    2012-07-01

    Previous studies have found a link between body tattoos or piercings and risky behavior. However, these studies only examined survey data but not real behavior. Young men (mean = 20.6 years) and women (mean = 20.2 years) leaving a bar were asked whether they wore tattoos and piercings or not and were requested to breathe into a breathalyzer in order to evaluate their alcohol consumption. It was found that participants with piercings and/or tattoos as well as combined piercings and tattoos revealed higher levels of alcohol consumption. Piercings and tattoos could serve as signs of alcohol consumption for educators, parents, and physicians. Copyright © 2012 by the Research Society on Alcoholism.

  4. Changing practices of energy consumption

    DEFF Research Database (Denmark)

    Christensen, Toke Haunstrup; Friis, Freja; Skjølsvold, Tomas Moe

    2017-01-01

    to produce hydrogen for transport use), whereas others emphasise the role of individual consumers. The latter approach is dominant within the smart grid vision. In this paper, we explore implications of smart grid technologies in households for the everyday practices related to electricity consumption...... (microgeneration) influence the everyday practices? What kind of influence does the combination of PVs with other “smart” energy technologies have on everyday practices and electricity consumption patterns? A specific focus is on the time patterns of households’ energy consumption. The analysis is based...... settlement scheme (hourly versus annual net metering) and the trial context play a role. Also, the study finds a broader interest in increasing the level of self-sufficiency through combining PVs with home batteries. Finally, the paper discusses a distinct (male) gendering in relation to who is most actively...

  5. LTE UE Power Consumption Model

    DEFF Research Database (Denmark)

    Jensen, Anders Riis; Lauridsen, Mads; Mogensen, Preben

    2012-01-01

    is based on a review of the major power consuming parts in an LTE UE radio modem. The model includes functions of UL and DL power and data rate. Measurements on a commercial LTE USB dongle were used to assign realistic power consumption values to each model parameter. Verification measurements......In this work a novel LTE user equipment (UE) power consumption model is presented. It was developed for LTE system level optimization, because it is important to understand how network settings like scheduling of resources and transmit power control affect the UE’s battery life. The proposed model...... on the dongle show that the model results in an average error of 2.6%. The measurements show that UL transmit power and DL data rate determines the overall power consumption, while UL data rate and DL receive power have smaller impact....

  6. Moral Violations Reduce Oral Consumption.

    Science.gov (United States)

    Chan, Cindy; Van Boven, Leaf; Andrade, Eduardo B; Ariely, Dan

    2014-07-01

    Consumers frequently encounter moral violations in everyday life. They watch movies and television shows about crime and deception, hear news reports of corporate fraud and tax evasion, and hear gossip about cheaters and thieves. How does exposure to moral violations influence consumption? Because moral violations arouse disgust and because disgust is an evolutionarily important signal of contamination that should provoke a multi-modal response, we hypothesize that moral violations affect a key behavioral response to disgust: reduced oral consumption. In three experiments, compared with those in control conditions, people drank less water and chocolate milk while (a) watching a film portraying the moral violations of incest, (b) writing about moral violations of cheating or theft, and (c) listening to a report about fraud and manipulation. These findings imply that "moral disgust" influences consumption in ways similar to core disgust, and thus provide evidence for the associations between moral violations, emotions, and consumer behavior.

  7. Synthesis of 2-Phenylazonaphtho[1,8-ef][1,4]diazepines and 9-(3-Arylhydrazonopyrrolo[1,2-a]perimidines as Antitumor Agents

    Directory of Open Access Journals (Sweden)

    Thoraya A. Farghaly

    2014-01-01

    Full Text Available Two series of naphtho[1,8-ef][1,4]diazepines and pyrrolo[1,2-a]perimidines were prepared starting from 1,8-diaminonaphthalene and hydrazonoyl chlorides. The structures of the products were determined on the basis of their spectral data and elemental analyses. The mechanism of formation of such products was also discussed. The prepared compounds were screened for their antitumor activity against three cell lines, namely, MCF-7, TK-10 and UACC-62, and some derivatives showed promising activity.

  8. A salivary EF-hand calcium-binding protein of the brown planthopper Nilaparvata lugens functions as an effector for defense responses in rice

    OpenAIRE

    Ye, Wenfeng; Yu, Haixin; Jian, Yukun; Zeng, Jiamei; Ji, Rui; Chen, Hongdan; Lou, Yonggen

    2017-01-01

    The brown planthopper (BPH), Nilaparvata lugens (St?l) (Hemiptera: Delphacidae), a major pest of rice in Asia, is able to successfully puncture sieve tubes in rice with its piercing stylet and then to ingest phloem sap. How BPH manages to continuously feed on rice remains unclear. Here, we cloned the gene NlSEF1, which is highly expressed in the salivary glands of BPH. The NlSEF1 protein has EF-hand Ca2+-binding activity and can be secreted into rice plants when BPH feed. Infestation of rice ...

  9. Results from a model system of superconducting solenoids and phase shifting bridge for pulsed power studies for proposed tokamak EF coils

    International Nuclear Information System (INIS)

    Fuja, R.E.; Kustom, R.L.; Smith, R.P.

    1977-01-01

    A matched pair of superconducting solenoids and a phase-shifting bridge circuit has been constructed to study energy storage and transfer for application to tokamak EF coils. The intrinsically stable solenoids, each with 4 H self-inductance, incorporate sufficient cooling to allow charging at several hundred volts, corresponding to B approximately equal 1 T/sec. The three-phase inductor-convertor capacitive bridge network operating at up to 150 V rms transfers energy reversibly and at controllable rates from the storage coil to the load coil

  10. Results from a model system of superconducting solenoids and phase shifting bridge for pulsed power studies for proposed tokamak EF coils

    International Nuclear Information System (INIS)

    Fuja, R.E.; Kustom, R.L.; Smith, R.P.

    1977-01-01

    A matched pair of superconducting solenoids and a phase-shifting bridge circuit has been constructed to study energy storage and transfer for application to tokamak EF coils. The intrinsically stable solenoids, each with 4 H self-inductance, incorporate sufficient cooling to allow charging at several hundred volts, corresponding to B = 1 T/sec. The three-phase inductor-convertor capacitive bridge network operating at up to 150 V rms transfers energy reversibly and at controllable rates from the storage coil to the load coil

  11. A Medicago truncatula EF-hand family gene, MtCaMP1, is involved in drought and salt stress tolerance.

    Directory of Open Access Journals (Sweden)

    Tian-Zuo Wang

    Full Text Available BACKGROUND: Calcium-binding proteins that contain EF-hand motifs have been reported to play important roles in transduction of signals associated with biotic and abiotic stresses. To functionally characterize genes of EF-hand family in response to abiotic stress, an MtCaMP1 gene belonging to EF-hand family from legume model plant Medicago truncatula was isolated and its function in response to drought and salt stress was investigated by expressing MtCaMP1 in Arabidopsis. METHODOLOGY/PRINCIPAL FINDINGS: Transgenic Arabidopsis seedlings expressing MtCaMP1 exhibited higher survival rate than wild-type seedlings under drought and salt stress, suggesting that expression of MtCaMP1 confers tolerance of Arabidopsis to drought and salt stress. The transgenic plants accumulated greater amounts of Pro due to up-regulation of P5CS1 and down-regulation of ProDH than wild-type plants under drought stress. There was a less accumulation of Na(+ in the transgenic plants than in WT plants due to reduced up-regulation of AtHKT1 and enhanced regulation of AtNHX1 in the transgenic plants compared to WT plants under salt stress. There was a reduced accumulation of H2O2 and malondialdehyde in the transgenic plants than in WT plants under both drought and salt stress. CONCLUSIONS/SIGNIFICANCE: The expression of MtCaMP1 in Arabidopsis enhanced tolerance of the transgenic plants to drought and salt stress by effective osmo-regulation due to greater accumulation of Pro and by minimizing toxic Na(+ accumulation, respectively. The enhanced accumulation of Pro and reduced accumulation of Na(+ under drought and salt stress would protect plants from water default and Na(+ toxicity, and alleviate the associated oxidative stress. These findings demonstrate that MtCaMP1 encodes a stress-responsive EF-hand protein that plays a regulatory role in response of plants to drought and salt stress.

  12. The CREC family, a novel family of multiple EF-hand, low-affinity Ca(2+)-binding proteins localised to the secretory pathway of mammalian cells

    DEFF Research Database (Denmark)

    Honoré, B; Vorum, H

    2000-01-01

    The CREC family consists of a number of recently discovered multiple (up to seven) EF-hand proteins that localise to the secretory pathway of mammalian cells. At present, the family includes reticulocalbin, ERC-55/TCBP-49/E6BP, Cab45, calumenin and crocalbin/CBP-50. Similar proteins are found......(2+)-regulated activities. Recent evidence has been obtained that some CREC family members are involved in pathological activities such as malignant cell transformation, mediation of the toxic effects of snake venom toxins and putative participation in amyloid formation. Udgivelsesdato: 2000-Jan-21...

  13. Coinheritance of High Oxygen Affinity Hb Helsinki [HBB: c.248A>T; β82(EF6)Lys→Met] with Hb H Disease.

    Science.gov (United States)

    Lee, Shir-Ying; Goh, Jia-Hui; Tan, Karen M L; Liu, Te-Chih

    2017-05-01

    Hb Helsinki [HBB: c.248A>T; β82(EF6)Lys→Met] is a high oxygen affinity hemoglobin (Hb) causing polycythemia, whereas Hb H (β4) disease causes mild to severe chronic hemolytic anemia. The clinical characteristics, gel electrophoresis, capillary electrophoresis (CE) and molecular genotyping of a case of Hb Helsinki coinherited with Hb H disease in an ethnic Malay is described, illustrating the interaction between the β-globin variant and coinheritance of three α gene deletions. The proband was asymptomatic, exhibited microcytosis and a normal with Hb value.

  14. Towards real-time eruption forecasting in the Auckland Volcanic Field: application of BET_EF during the New Zealand National Disaster Exercise `Ruaumoko'

    Science.gov (United States)

    Lindsay, Jan; Marzocchi, Warner; Jolly, Gill; Constantinescu, Robert; Selva, Jacopo; Sandri, Laura

    2010-03-01

    The Auckland Volcanic Field (AVF) is a young basaltic field that lies beneath the urban area of Auckland, New Zealand’s largest city. Over the past 250,000 years the AVF has produced at least 49 basaltic centers; the last eruption was only 600 years ago. In recognition of the high risk associated with a possible future eruption in Auckland, the New Zealand government ran Exercise Ruaumoko in March 2008, a test of New Zealand’s nation-wide preparedness for responding to a major disaster resulting from a volcanic eruption in Auckland City. The exercise scenario was developed in secret, and covered the period of precursory activity up until the eruption. During Exercise Ruaumoko we adapted a recently developed statistical code for eruption forecasting, namely BET_EF (Bayesian Event Tree for Eruption Forecasting), to independently track the unrest evolution and to forecast the most likely onset time, location and style of the initial phase of the simulated eruption. The code was set up before the start of the exercise by entering reliable information on the past history of the AVF as well as the monitoring signals expected in the event of magmatic unrest and an impending eruption. The average probabilities calculated by BET_EF during Exercise Ruaumoko corresponded well to the probabilities subjectively (and independently) estimated by the advising scientists (differences of few percentage units), and provided a sound forecast of the timing (before the event, the eruption probability reached 90%) and location of the eruption. This application of BET_EF to a volcanic field that has experienced no historical activity and for which otherwise limited prior information is available shows its versatility and potential usefulness as a tool to aid decision-making for a wide range of volcano types. Our near real-time application of BET_EF during Exercise Ruaumoko highlighted its potential to clarify and possibly optimize decision-making procedures in a future AVF eruption

  15. Stochastic estimation of electricity consumption

    International Nuclear Information System (INIS)

    Kapetanovic, I.; Konjic, T.; Zahirovic, Z.

    1999-01-01

    Electricity consumption forecasting represents a part of the stable functioning of the power system. It is very important because of rationality and increase of control process efficiency and development planning of all aspects of society. On a scientific basis, forecasting is a possible way to solve problems. Among different models that have been used in the area of forecasting, the stochastic aspect of forecasting as a part of quantitative models takes a very important place in applications. ARIMA models and Kalman filter as stochastic estimators have been treated together for electricity consumption forecasting. Therefore, the main aim of this paper is to present the stochastic forecasting aspect using short time series. (author)

  16. GOVERNMENT INVOLVEMENT IN CONSUMPTION BEHAVIOUR

    Directory of Open Access Journals (Sweden)

    CRISTINA ZAMFIR

    2010-01-01

    Full Text Available In this article, we will follow the involvement that the government has,through its expenses, on the consumption behavior. The involvement that the government has inthe consumption behavior is made through fees and taxes that are applied on income. Fees andtaxes are applied to the different forms of income but in this article we will be focused only onthe influence of them on wages. In order to analyze the involvement of government expenses onconsumption behavior an utility model will be used.

  17. "Conscious Consumption": Ecocapitalism as Ideology

    Directory of Open Access Journals (Sweden)

    Maria das Graças e Silva

    2012-06-01

    Full Text Available The purpose of this article is to raise a set of questions about “conscious consumption.” It is an essay of a bibliographic nature whose central thesis consists in affirming that in a capitalist society conscious consumption cannot be instituted as an affirmation of the principle of socioenvironmental sustainability. The paper presents the ideological nature of this formulation, which associates consumerism and the possibility of overcoming it only to the need for behavioral changes, without explaining its socioeconomic dimensions and its functionality as a mechanism for the reproduction of the destructive logic of capital.

  18. Consumption patterns and cultural values in Europe

    NARCIS (Netherlands)

    Annemien van der Veen; Agnes Neulinger; Jacob Rosendahl; Tino Bech-Larsen

    2013-01-01

    Chapter 6 in Consumption culture in Europe. The chapter focuses on cultural differences in consumption across Europe and describes general attitudes towards consumption and brands, the significance of shopping, and how these are linked to the motives of consumption of alcoholic and non-alcoholic

  19. The world energy consumption in 2005

    International Nuclear Information System (INIS)

    Lapillonne, B.

    2006-01-01

    Based on Enerdata 2005 data, this analysis presents the situation of the world energy consumption in 2005, the electric power consumption per region and production per source, the consumption increase for each energy source and the petroleum and gas consumption increase. (A.L.B.)

  20. The industrial energy consumption in 2001

    International Nuclear Information System (INIS)

    Anon.

    2002-01-01

    The Danish industrial energy consumption in 2001 is presented in tables. The tables include: the development in the energy consumption, the amount of employees in each of the main branches, fuel consumption, the fuel and energy consumption in 2001 based on each group of branches and energy category, and the emission of CO 2 . (LN)

  1. Unrecorded Alcohol Consumption: Quantitative Methods of Estimation

    OpenAIRE

    Razvodovsky, Y. E.

    2010-01-01

    unrecorded alcohol; methods of estimation In this paper we focused on methods of estimation of unrecorded alcohol consumption level. Present methods of estimation of unrevorded alcohol consumption allow only approximate estimation of unrecorded alcohol consumption level. Tacking into consideration the extreme importance of such kind of data, further investigation is necessary to improve the reliability of methods estimation of unrecorded alcohol consumption.

  2. Evolution of food consumption in Romania

    Directory of Open Access Journals (Sweden)

    Elena Peț

    2014-05-01

    Full Text Available Closely connected to production, consumption plays an active catalyst role because all economic activities end in consumption. From this perspective, consumption research is a good solution to identify offer accurately and meet the needs and, on the other hand, it is an important source of information in marketing. Consumption ranges among the first in the process of goods use. Consumption is the process of using goods to meet consumption needs (though not all needs since there is always a gap between needs and consumption.

  3. The industrial energy consumption in 2003

    International Nuclear Information System (INIS)

    Anon.

    2004-01-01

    The statistics present the industry's energy consumption and composition, and the development from 1973 to 2003. In this period the composition of the energy consumption has changed considerably: a decrease in the consumption of liquid fuels and an increase in the consumption of natural gas and electric power. The energy consumption in the Danish industry decreased with almost 9 % from 2001 to 2003. This relatively large decrease was mainly due to the closing down of a steel factory. In the wood industry the energy consumption decreased with 36 % from 2001 to 2003, while the energy consumption in the electronics industry increased. (ln)

  4. Energy consumption 2005 with Danish industry

    International Nuclear Information System (INIS)

    Anon.

    2006-01-01

    The energy consumption in the Danish industries decreased with 4% from 2003 to 2005. The consumption of liquid fuels and district heat decreased with 27% and 21%, respectively. The consumption of solid fuels increased with 13%. The aim of the statistics is to elucidate the industry's energy consumption and its composition. The statistics present the development in the industry from 1973 to 2005, in which period the composition of the energy consumption has changed significantly. Especially, consumption of liquid fuels has decreased and consumption of gas and electricity has increased. (ln)

  5. Análise em microscopia eletrônica de varredura da superfície do esmalte dentário submetido à ação de sucos de frutas cítricas = Analysis in electronic microscopy of sweeping of the surface of the dental enamel submitted citric fruit juice action

    OpenAIRE

    Claudino, Lígia Vieira

    2006-01-01

    O presente trabalho objetivou avaliar a superfície do esmalte humano submetido à ação de diferentes sucos de frutas cítricas, em microscopia eletrônica de varredura (MEV). Foram selecionados 22 dentes extraídos por razões ortodônticas, livres de trincas e/ou fraturas, sendo obtidos 44 blocos de esmalte a partir das superfícies vestibulares e linguais/palatinas destes elementos. Os blocos foram aleatoriamente divididos em 10 grupos experimentais (n = 4), submetidos ao contato por 5 minutos com...

  6. Framework com as contribuições da convergência digital possibilitada pela utilização das tecnologias interativas da TV digital, associadas ao uso dos dispositivos móveis digitais, para a evolução do modelo brasileiro de governo eletrônico

    OpenAIRE

    Ferreira, Marcus Vinicius Anátocles da Silva

    2013-01-01

    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia e Gestão do Conhecimento, Florianópolis, 2013. Esta pesquisa tem como foco a concepção de um framework com as contribuições da convergência digital possibilitada pelas tecnologias interativas da TV Digital, associadas ao uso dos dispositivos móveis digitais, para a evolução do modelo de governo eletrônico no Brasil. A pesquisa utiliza como referência a validação do pressup...

  7. Protein consumptions in stroke patients

    Directory of Open Access Journals (Sweden)

    Zahra Maghsoudi

    2013-01-01

    Full Text Available Background : Stroke is one of the most common causes of disabilities and death all over the world. The mortality rate of stroke is predicted to be doubled by 2030 in the Middle East countries. Nutrition is an effective strategy in prevention and management of stroke. This study assessed the relationship between various protein types and stroke risk. Materials and Methods: This hospital-based case-control study was performed in a University hospital. The data regarding consumption of usual food intake of 69 cases (46 men and 23 women and 60 controls (30 men and 30 women was collected with a food frequency questionnaire (FFQ. The mean consumption of red and white meat and vegetable and processed proteins consumption were compared between two groups. Results: The percent of total of daily protein intake were lower in patients with stroke in both sexes (25.92% vs 30.55% in men and 30.7% vs 31.14% in women. Conclusion: Lower protein consumption may be observed in patients with stroke patients in both sex.

  8. Sales promotions and food consumption.

    Science.gov (United States)

    Hawkes, Corinna

    2009-06-01

    Sales promotions are widely used to market food to adults, children, and youth. Yet, in contrast to advertising, practically no attention has been paid to their impacts on dietary behaviors, or to how they may be used more effectively to promote healthy eating. This review explores the available literature on the subject. The objective is to identify if and what literature exists, examine the nature of this literature, and analyze what can be learned from it about the effects of sales promotions on food consumption. The review finds that while sales promotions lead to significant sales increases over the short-term, this does not necessarily lead to changes in food-consumption patterns. Nevertheless, there is evidence from econometric modeling studies indicating that sales promotions can influence consumption patterns by influencing the purchasing choices of consumers and encouraging them to eat more. These effects depend on the characteristics of the food product, sales promotion, and consumer. The complexity of the effects means that sales promotions aiming to encourage consumption of nutritious foods need to be carefully designed. These conclusions are based on studies that use mainly sales data as a proxy for dietary intake. The nutrition (and economics) research communities should add to this existing body of research to provide evidence on the impact of sales promotions on dietary intake and related behaviors. This would help support the development of a sales promotion environment conducive to healthy eating.

  9. Interventions to encourage sustainable consumption

    NARCIS (Netherlands)

    Dam, van Y.K.; Trijp, van J.C.M.

    2016-01-01

    Sustainable consumption is hampered by a discrepancy between consumers’ attitudes and their actual behaviour in the market place. Psychological construal level theory provides an explanation for the attitude to behaviour gap as a motivational conflict between high and low level of mental construal.

  10. Energy Consumption vs. Energy Requirement

    Science.gov (United States)

    Fan, L. T.; Zhang, Tengyan; Schlup, John R.

    2006-01-01

    Energy is necessary for any phenomenon to occur or any process to proceed. Nevertheless, energy is never consumed; instead, it is conserved. What is consumed is available energy, or exergy, accompanied by an increase in entropy. Obviously, the terminology, "energy consumption" is indeed a misnomer although it is ubiquitous in the…

  11. Energy Consumption Management in Design

    NARCIS (Netherlands)

    Smit, Jaap

    1997-01-01

    A survey of the basic issues in low power design is presented, including techniques for the analysis of energy consumption in the early design phase of analog and digital circuits. The concept of energy complexity will be introduced in conjunction with techniques for parameterized energy management.

  12. Back to consumption and production?

    DEFF Research Database (Denmark)

    Bajde, Domen; Kos Koklic, Mateja; Bajde, Jernej

    2015-01-01

    of production and consumption. Yet, in response to the mounting commercial and legal pressures, Web 2.0 companies pro-actively regulate prosumption, deploying advanced technological devices and innovative contractual arrangements. Exploring the case of Warner Music Group lockdown on YouTube, we investigate...

  13. The Ecological Economics of Consumption

    DEFF Research Database (Denmark)

    Røpke, Inge; Reisch, Lucia A.

    In accordance with the transdisciplinary basis of ecological economics, this volume encompasses contributions from different perspectives, often cutting across disciplines. It is divided into three parts that group contributions on · problematizing consumption both as a concept and as an economic...

  14. Does Consumption Lag Behind Incomes?

    NARCIS (Netherlands)

    J. Tinbergen (Jan)

    1942-01-01

    textabstractThe fact that consumption outlay of individuals as well as of groups of individuals depends on their income is well known. Although this statement will hardly be doubted, it may be tested statistically from family budget statistics, as has been done by various investigators. These

  15. Consumption, lifestyles and constellatoric strategies

    DEFF Research Database (Denmark)

    Læssøe, Jeppe

    1996-01-01

    The condtribution reveals a contradiction between complexity and operationality in the discussions about energy consumption between social researchers and policy makers. By mean of two examples from his own research the author argues for a transgression of the narrow ecology-centred view. The first...... of "constellatoric strategies"; coordinated interplays between different coordinated efforts which creates synergy effects....

  16. Investigation into the Formation, Structure, and Evolution of an EF4 Tornado in East China Using a High-Resolution Numerical Simulation

    Science.gov (United States)

    Yao, Dan; Xue, Haile; Yin, Jinfang; Sun, Jisong; Liang, Xudong; Guo, Jianping

    2018-04-01

    Devastating tornadoes in China have received growing attention in recent years, but little is known about their formation, structure, and evolution on the tornadic scale. Most of these tornadoes develop within the East Asian monsoon regime, in an environment quite different from tornadoes in the U.S. In this study, we used an idealized, highresolution (25-m grid spacing) numerical simulation to investigate the deadly EF4 (Enhanced Fujita scale category 4) tornado that occurred on 23 June 2016 and claimed 99 lives in Yancheng, Jiangsu Province. A tornadic supercell developed in the simulation that had striking similarities to radar observations. The violent tornado in Funing County was reproduced, exceeding EF4 (74 m s-1), consistent with the on-site damage survey. It was accompanied by a funnel cloud that extended to the surface, and exhibited a double-helix vorticity structure. The signal of tornado genesis was found first at the cloud base in the pressure perturbation field, and then developed both upward and downward in terms of maximum vertical velocity overlapping with the intense vertical vorticity centers. The tornado's demise was found to accompany strong downdrafts overlapping with the intense vorticity centers. One of the interesting findings of this work is that a violent surface vortex was able to be generated and maintained, even though the simulation employed a free-slip lower boundary condition. The success of this simulation, despite using an idealized numerical approach, provides a means to investigate more historical tornadoes in China.

  17. La fiesta peronista: recorridos y apropiaciones entre fotografía, arquitectura efímera y poder político

    Directory of Open Access Journals (Sweden)

    Franco Marchionni Sánchez

    2016-04-01

    Full Text Available El objetivo de este trabajo es analizar la relación entre algunos de los escenarios, fotografías y afiches utilizados como parte de las estrategias desarrolladas por el aparato de administración peronista y explicar su incidencia en la Fiesta de la Vendimia y su construcción imaginaria en torno de la “nueva Argentina” después de la Segunda Guerra Mundial. En este momento histórico particular, los registros gráficos y fotográficos considerados son una puerta de entrada para el análisis de los fenómenos efímeros que no pueden ser recuperados de otro modo. La estrategia metodológica utilizada es cualitativa y de tipo exploratorio, con un diseño de carácter flexible. Si bien estos testimonios, reflejados en las fuentes descriptas, no nos devuelven la posibilidad de estar en contacto directo con esas experiencias, sino que nos permiten acceder al conjunto de deseos, tensiones, frustraciones, expectativas, debates, los éxitos y los fracasos mediante los cuales se formularon y desarrollaron los proyectos de arquitectura escénica y propaganda política.   Palabras clave: Archivos fotográficos; Arquitectura Efímera; Fiesta de la Vendimia; Relaciones de Poder; Peronismo.

  18. Energy consumption trends in Hawaii

    International Nuclear Information System (INIS)

    Kaya, Abidin; Yalcintas, Melek

    2010-01-01

    This study begins with a review of energy consumption by end-use sector in Hawaii. Then, the energy generated from renewable energy sources is analyzed between 1991 and 2006. The results show that while geothermal is a considerable source of renewable energy on the Island of Hawaii (also known as Big Island), fossil fuel is the main energy source in the State of Hawaii. The energy intensity index for the State of Hawaii is then calculated by dividing energy consumption per capita by the income per capita. The calculated energy intensity index reveals that energy consumption is directly controlled by per capita income. The results also indicate that the energy intensity index increases over time despite positive developments in energy efficient technologies. In the second part of the paper, the effect of the tourism industry on energy usage in the State of Hawaii is analyzed. The results show that tourism volume, measured in terms of tourist arrival numbers, does not change the energy consumption directly. However, a change in tourism volume does affect per capita income within a few months to a year. In the last part of the study, the energy efficiency index of Hawaii is compared with consumption averages for the US, California and the most energy efficient country in Europe, Denmark. The comparison shows that Hawaii lags behind California and Denmark in terms of energy efficiency. The comparison also shows that an increase in energy efficiency corresponds to an increase in per capita income across the board, which is in agreement with a recent report published by the American Physical Society.

  19. Snail meat: Significance and consumption

    Directory of Open Access Journals (Sweden)

    Dragićević Olgica

    2005-01-01

    Full Text Available The consumption of snail meat goes back to prehistoric times. Different ancient nations had snails on their menu, but Helices culture as a productive activity was born as a Roman culture. Some of the most economically important edible species are: Helix aspersa (Mtiller Helixpomatia (Linne, Helix iucorum (Linne, Helix aperta (Born, Eobania vermiculata (Miiller. Together with its tasie, snail meat has several advantages over others: quite low lipid rate and calorie values versus rich mineral, essential amino acid and fatty acid content. The composition of snail meat is presented. In addition, the composition of different snail species and the part analyzed (pedal mass and visceral mass is presented. Also, the differences in composition according to the species (snail meat horse/chicken meat, beef, swine meat, fish meat are presented. The French are the world's leading consumers of snails. !n France snails come to market in a variety of ways. Estimated consumption of snails in France is around 40 000 tones/year. Total French imports account for 25% of world imports. France is also the leading exporter of prepared snails, mainly sold as preserved snails and prepared dishes. Snail imports have been much higher than exports (65 tones exported in 2002. vs. 2.700 tones imported. Despite the large consumption, only 3% of snails in France come from production (farming. Italy is in second place in the world consumption of snails, and Spain and Germany are in the third and fourth place. The development of snails consumption in Italy is followed with the same amount of production of snails in the whole biological circle. In 2001, from 24,700 tons, 9,350 tons (37.8% came from production, 6 00 tons (2.4% came from nature, and 14,750 tons (59.70% came from imports (frozen, fresh and prepared snails. In Serbia, at the beginning of 2005, we had over 400 registered farms for snail production.

  20. Hypercoagulability after energy drink consumption.

    Science.gov (United States)

    Pommerening, Matthew J; Cardenas, Jessica C; Radwan, Zayde A; Wade, Charles E; Holcomb, John B; Cotton, Bryan A

    2015-12-01

    Energy drink consumption in the United States has more than doubled over the last decade and has been implicated in cardiac arrhythmias, myocardial infarction, and even sudden cardiac death. We hypothesized that energy drink consumption may increase the risk of adverse cardiovascular events by increasing platelet aggregation, thereby resulting in a relatively hypercoagulable state and increased risk of thrombosis. Thirty-two healthy volunteers aged 18-40 y were given 16 oz of bottled water or a standardized, sugar-free energy drink on two separate occasions, 1-wk apart. Beverages were consumed after an overnight fast over a 30-min period. Coagulation parameters and platelet function were measured before and 60 min after consumption using thrombelastography and impedance aggregometry. No statistically significant differences in coagulation were detected using kaolin or rapid thrombelastography. In addition, no differences in platelet aggregation were detected using ristocetin, collagen, thrombin receptor-activating peptide, or adenosine diphosphate-induced multiple impedance aggregometry. However, compared to water controls, energy drink consumption resulted in a significant increase in platelet aggregation via arachidonic acid-induced activation (area under the aggregation curve, 72.4 U versus 66.3 U; P = 0.018). Energy drinks are associated with increased platelet activity via arachidonic acid-induced platelet aggregation within 1 h of consumption. Although larger clinical studies are needed to further address the safety and health concerns of these drinks, the increased platelet response may provide a mechanism by which energy drinks increase the risk of adverse cardiovascular events. Copyright © 2015 Elsevier Inc. All rights reserved.

  1. Dosagem de marcadores cardíacos CK-MB e TnIc e eletrólitos no envenenamento experimental por veneno de sapo em cães

    Directory of Open Access Journals (Sweden)

    Annelise C. Camplesi

    2009-08-01

    Full Text Available Dentre os sinais sistêmicos causados pelo envenenamento por veneno de sapo (bufotoxina em cães, os efeitos cardiotóxicos são um dos mais importantes. O objetivo deste estudo foi avaliar as potenciais alterações no músculo cardíaco de cães envenenados experimentalmente por veneno de sapo e observar as alterações eletrolíticas que podem ocorrer nesse tipo de envenenamento. Utilizaram-se 20 cães divididos em grupo controle (n=5 e grupo envenenado (n=15. O veneno de sapo foi extraído por meio de compressão manual das glândulas paratóides. Após anestesia geral, os cães do grupo controle receberam placebo (solução fisiológica e os do grupo envenenado uma alíquota do veneno por sonda orogástrica. As colheitas de sangue para dosagem dos marcadores cardíacos foram realizadas seis e 24 horas após o envenenamento. As colheitas de sangue para dosagem dos eletrólitos foram realizadas antes e duas, quatro, seis e 12 horas após o envenenamento. A análise estatística empregada foi o teste não-paramétrico de Mann-Withney (PAmong the systemic signs of toad venom (bufotoxin poisoning in dogs, the cardiotoxic effects are one of the most important. Thus, the objective of this experiment was to evaluate potential changes in the cardiac muscle in dogs poisoned experimentally by toad venom and to observe the eletrolyte alterations which may occur in this condition. Twenty dogs divided into control group (n=5 and poisoned group (n=15 were utilized. The toad venom was extracted by manual compression of the paratoidic glands. After general anesthesia, dogs in the control group received placebo and dogs in the poisoned group received the venom by orogastric catheter. Samples for dosage were collected 6 hours and 24 hours after poisoning and 0, 2, 4, 6 and 12 hours after poisoning for electrolytes dosage. The Man-Withney test was used for statistical analysis (P<0.05. The poisoned dogs showed (saline elevated levels of cardiac markers CK

  2. Logística e troca eletrônica de informação em empresas automobilísticas e alimentícias Logistics and electronic information interchange in automotive and food companies

    Directory of Open Access Journals (Sweden)

    Karine Araújo Ferreira

    2005-12-01

    Full Text Available Este artigo analisa impactos na logística pela troca eletrônica de informações entre empresas (com uso de EDI - Electronic Data Interchange e Internet, destacando a diferença destes impactos em empresas das indústrias alimentícia e automobilística. As empresas são pressionadas por parceiros para adoção destas tecnologias: a montadora é a responsável na cadeia automobilística e o grande varejo é o disseminador na cadeia alimentícia. O EDI pressupõe relações contratuais entre as empresas. As processadoras de alimentos observaram redução de erros e no tempo dos ciclos de pedido, assim como melhoria na visibilidade dos estoques e no planejamento de transporte para distribuição de seus produtos. Já nas empresas da indústria automobilística, os impactos são mais significativos no tempo e custo de processamento de pedido, com redução nos níveis e custo de estoque e de transporte para suprimento. Os resultados são de pesquisa de natureza qualitativa - estudos de casos.This paper analyzes the impacts of electronic information exchange (EDI - Electronic Data Interchange and Internet toward companies' logistics performance, emphasizing the difference of these impacts within food and automobile industries. Companies are being impelled by their partners to adopt these information technologies, whether: vehicle assemblers are responsible for the automobile chain; and great retailers work as disseminators through the food chain. EDI's implementation requires contractual relations among companies. Therefore, food processing industries aim to reduce both errors and cycle-time orders; as well as to improve inventory visibility and transport planning in the distribution of its products. However, in the automobile companies segment, the impacts are more significant in time and order processing costs, with the reduction of inventory and transport level's costs for supplying. The presented results are of qualitative research nature

  3. Características das papilas o dorso da língua de cabras (Capra hircus: estudo por de microscopia eletrônica de varredura e luz

    Directory of Open Access Journals (Sweden)

    Erika Toledo da Fonseca

    2011-12-01

    Full Text Available A morfologia das papilas linguais da cabra doméstica (Capra hircus foi estudada por Microscopia Eletrônica de Varredura (MEV e Microscopia de luz. Papilasfiiliformes, cônicas e lentiformes possuem função mecânica, enquanto que as papilas fungiformes e as valadas possuem função gustativa. As papilasfiiliformes estavam distribuídas por toda a extensão da língua, nas superfícies dorsal e lateral. Possuem um formato cônico com a extremidade pontiaguda direcionada caudalmente. Possuem vários processos secundários, de tamanhos diferentes, com a mesma orientação. A histologia revelou abundante tecido queratinizado. As papilas fungiformes foram observadas nas superfícies dorsal e lateral do ápice e do corpo da língua. Possuem formato semelhante a cogumelos, com epitélio corni[1]icado espesso e botões gustativos distribuídos na superfície. As papilas lentiformes se distribuíam na linha mediana da parte mais dorsal do toro, com projeções elevadas além da superfície da língua. Algumas apresentavam o formato de pirâmide e outras um formato mais achatado. As papilas valadas foram encontradas na superfície lateral da parte mais caudal do toro. Possuem formato arredondado, envolvido por um sulco pouco profundo. Possuem botões gustativos na parede lateral que se abrem no sulco. As papilas cônicas foram observadas no toro da língua e possuem formato alongado, com base larga e ponta romba. Embora as principais características morfológicas e estruturais do epitélio lingual sejam específicas da espécie, o tipo de alimentação e os hábitos alimentares podem influenciar na sua estrutura. Assim, o presente trabalho fornece uma descrição histológica e ultraestrutural da língua de cabras domésticas submetidas à alimentação baseada em gramíneas e ração.

  4. Prescrição off-label, direito à informação, consentimento informado e processo clinico eletrónico no direito português

    Directory of Open Access Journals (Sweden)

    Carla Barbosa

    2016-09-01

    Full Text Available A prescrição off-label de medicamentos é não só uma prática comum e amplamente aceite, como também incontornável nos sistemas de saúde modernos. Perante a ausência de terapia eficaz, o médico não pode ficar sujeito ao tempo necessário para que todo o processo de aprovação de um determinado uso para um determinado medicamento se desenvolva. No entanto, apesar de se mostrar urgente, ainda não existe uma regulamentação desse tipo de receita médica em Portugal (ou mesmo na Europa. Como uma prerrogativa do médico, a liberdade terapêutica deve garantir o respeito pelo paciente, razão pela qual deve ser plenamente informado. É imperativo que o paciente conheça aquela terapia particular, os seus potenciais riscos e benefícios e as razões que levaram o médico a prescrever off-label. Todas as informações devem, por isso, ser claramente anotadas nos registos clínicos do paciente de modo garantir uma efetiva proteção tanto para o paciente como para o médico. Embora não exista, ainda, consenso quanto à prescrição off-label, em 2014 a Direção Geral de Saúde portuguesa emitiu uma norma que recomenda a obtenção do consentimento informado, sob a forma escrita, para os casos de uso off-label de medicamentos. O problema reside na seguinte questão: como ajustar essa recomendação com a crescente informatização dos registros clínicos e com mecanismos como a Plataforma de Dados de Saúde Português? Na verdade, este é um problema comum. Recentemente, a FDA (Food and Drug Administration, EUA publicou um projeto que fornece, precisamente, recomendações sobre procedimentos utilizando um consentimento informado eletrónico.

  5. Clinical variability in dystrophic epidermolysis bullosa and findings with scanning electron microscopy Variabilidade clínica em epidermólise bolhosa distrófica e achados de microscopia eletrônica de varredura

    Directory of Open Access Journals (Sweden)

    Hiram Larangeira de Almeida Jr

    2012-02-01

    Full Text Available In dystrophic epidermolysis bullosa, the genetic defect of anchoring fibrils leads to cleavage beneath the basement membrane and its consequent loss. A 46 year-old female patient presented blisters with a pretibial distribution associated with nail dystrophy. Her two children had hyponychia and anonychia, which affected all toe nails and the thumb, forefinger and middle finger. DNA sequencing identified in exon 75 of COL7A1 gene a pathologic mutation: c.6235G>A (p.Gly2079Arg. Immunomapping of a blister demonstrated collagen IV (basal membrane in the blister roof and collagen VII in its floor, confirming dystrophic epidermolysis bullosa. Scanning electron microscopy of an inverted blister showed net-forming collagen attached to the blister roof . The variability found in this family has already been reported and confirms, on a clinical basis, the nail subtype as a dystrophic variant.Na epidermólise bolhosa distrófica, o defeito genético das fibrilas de ancoragem leva à clivagem abaixo da membrana basal com sua consequente perda. Uma paciente de 46 anos apresentava bolhas pré-tibiais associadas à distrofia ungueal. Seus dois filhos apresentavam hipo e anoníquia, afetando todas as unhas dos pododáctilos e dos primeiros, segundos e terceiros quirodáctilos. O sequenciamento de DNA identificou no exon 75 do gene COL7A1 uma mutação patológica: c.6235G>A (p.Gly2079Arg. O imunomapeamento identificou o colágeno IV no teto e colágeno VII no assoalho de uma bolha , confirmando o diagnóstico de epidermólise bolhosa distrófica. A microscopia eletrônica de varredura de um teto invertido de bolha demonstrou rede de colágeno aderida ao mesmo. A variabilidade clínica encontrada nessa família já foi escrita e confirma, que o subtipo ungueal das epidermólises bolhosas é uma forma distrófica.

  6. Análise morfológica da placenta da paca (Agouti paca, Linnaeus, 1766: Estudo ao microscópio de luz e à microscopia eletrônica de transmissão

    Directory of Open Access Journals (Sweden)

    Marina Bonatelli

    2001-01-01

    Full Text Available Foram estudados os aspectos morfológicos de nove placentas de paca (Agouti paca, L., 1766 mediante análises em microscopia de luz e eletrônica de transmissão dos fragmentos teciduais correspondentes à porção de maior conexão placentária em diferentes fêmeas gestantes, nos estágios intermediário e final da prenhez. Realizamos este estudo, pois, aliada à necessidade da procura de novas espécies que atuem como modelos experimentais adequados, havia a disponibilidade deste roedor em nosso meio; por outro lado, o melhor conhecimento dos aspectos reprodutivos destes animais oferece subsídios ao estabelecimento de criatórios racionais desta espécie, uma vez que a preservação deste vertebrado é necessária, além do grande interesse comercial em torno de sua carne. Os resultados mostraram que este roedor possui uma placenta do tipo vitelina e outra do tipo corioalantoidiana, sendo este órgão do tipo hemocorial, labiríntico, que se apresenta histologicamente composto por lóbulos divididos em três regiões distintas: o centro do lóbulo, o labirinto e o interlóbulo. Na região do centro do lóbulo, verificou-se a presença de artérias e veias; e em sua região periférica estavam presentes dois sistemas tubulares arranjados de forma paralela, onde as lacunas sangüíneas e os capilares estavam em íntimo contato, formando a região do labirinto. O interlóbulo era composto de artérias e veias. O trofoblasto era o principal componente da placenta, que, independentemente da região onde se encontrava, mostrava-se de natureza sincicial. Ultra-estruturalmente a barreira placentária da paca foi classificada como hemomonocorial.

  7. Forestry: Prospective Scenarios for Generating Eletricity

    Directory of Open Access Journals (Sweden)

    Henrique Dias Blois

    2017-05-01

    Full Text Available Forestry generates in all production segments a lot of waste which is a potential power source and an opportunity for electricity generation by the use of this material. Therefore, this study represents the construction of future scenarios for the power generation sector from alternative and renewable sources in the region of Vale do Taquari and Alto da Serra do Botucaraí, in the Northern-Central region of the Stat of Rio Grande do Sul, aiming for the application of waste and by-products from forestry present in a intensive and tendentious way in economic activities within the region. By way of prospective scenarios, one will be able to observe which events are impactful on the viability of an environmental project for recovery of forestry waste to generate electricity in the region. Thereby, we used the method described by Grumbach (2000, a methodology that prospects scenarios from the participation of experts, who collaborated analyzing and proposing events that impact as strengths and weaknesses, opportunities and threats and their interrelationships. Also, it was evident the degree of influence and dependence between the events, which create a matrix of cross impacts. Moreover, possible future scenarios have been constructed and analyzed for demonstrating that the study area has potential events that could serve as alternatives to the sector. However, no ideal scenario has been identified for the next five years, a fact that is due to the large amount of adverse events in six out of the ten final events.

  8. Global risks from energy consumption

    International Nuclear Information System (INIS)

    von Hippel, F.

    1983-01-01

    A discussion of some of the global risks associated with current and frequently proposed future levels of consumption of energy from oil, coal, fission, fusion, and renewable sources points out the the dangers are serious and relatively near term. These include world war over Persian Gulf oil, climate change due to the buildup of atmospheric carbon dioxide, the accelerated proliferation of nuclear weapons, and competition between food and energy for land and water. The author urges placing a greater emphasis on how we use energy and how to reduce energy waste. At the levels of consumption which economically justified levels of energy efficiency could bring about, enough flexibility could develop in our choice of a future energy-supply mix to dramatically reduce the associated global risks. 47 references, 3 figures

  9. ALMR potential for actinide consumption

    International Nuclear Information System (INIS)

    Cockey, C.L.; Thompson, M.L.

    1992-01-01

    The Advanced Liquid Metal Reactor (ALMR) is a US Department of Energy (DOE) sponsored fast reactor design based on the Power Reactor, Innovative Small Module (PRISM) concept originated by General Electric. This reactor combines a high degree of passive safety characteristics with a high level of modularity and factory fabrication to achieve attractive economics. The current reference design is a 471 MWt modular reactor fueled with ternary metal fuel. This paper discusses actinide transmutation core designs that fit the design envelope of the ALMR and utilize spent LWR fuel as startup material and for makeup. Actinide transmutation may be accomplished in the ALMR core by using either a breeding or burning configuration. Lifetime actinide mass consumption is calculated as well as changes in consumption behavior throughout the lifetime of the reactor. Impacts on system operational and safety performance are evaluated in a preliminary fashion. Waste disposal impacts are discussed. (author)

  10. Manufacturing consumption of energy 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-01

    This report provides estimates on energy consumption in the manufacturing sector of the U.S. economy based on data from the Manufacturing Energy Consumption Survey. The sample used in this report represented about 250,000 of the largest manufacturing establishments which account for approximately 98 percent of U.S. economic output from manufacturing, and an expected similar proportion of manufacturing energy use. The amount of energy use was collected for all operations of each establishment surveyed. Highlights of the report include profiles for the four major energy-consuming industries (petroleum refining, chemical, paper, and primary metal industries), and an analysis of the effects of changes in the natural gas and electricity markets on the manufacturing sector. Seven appendices are included to provide detailed background information. 10 figs., 51 tabs.

  11. Raw milk consumption and health

    Directory of Open Access Journals (Sweden)

    Popović-Vranješ Anka

    2015-01-01

    Full Text Available Contrary to the safe practices of milk pasteurization or sterilization, which effectively reduce foodborne outbreaks incidence associated with raw milk and dairy products use, outbreaks caused by such products continue to occur. Despite this fact, a worldwide movement advocating for the rights of raw milk and cheese selling and consumption, due to their specific nutritive characteristics, has strengthened significantly in recent years. Traditional agricultural manufacturers from Serbia still sell products related to thermally unprocessed milk, such as cottage cheese and raw cream. In AP Vojvodina during the period of 1981-2010 a total of 179 foodborne outbreaks were reported, where the incriminated cause of the outbreak were milk or diary. In 126 (70.39% outbreaks, totaling 2276 sick individuals and one casualty, it was confirmed that the incriminated food was from the group of dairy products. In 48 instances (26.82%, bacteriological tests confirmed that milk and dairy products were excluded as the outbreak causes, while in another 5 (2.79% outbreaks, microbiological analysis of food failed to confirm any relation to the actual epidemiological instances. In some cases, bacteriological testing of incriminated foods was not possible. In the cases of outbreaks associated with the consumption of milk and dairy products, traditional raw milk products were cited as being used. Consumption of unpasteurized milk and cheese represents public health threat. National and international rules ensuring use of safe products for human consumption have to set rules of trade of thermally processed milk and products on the market. [Projekat Ministarstva nauke Republike Srbije, br. TR31095

  12. Stuttering, alcohol consumption and smoking.

    Science.gov (United States)

    Heelan, Milly; McAllister, Jan; Skinner, Jane

    2016-06-01

    Limited research has been published regarding the association between stuttering and substance use. An earlier study provided no evidence for such an association, but the authors called for further research to be conducted using a community sample. The present study used data from a community sample to investigate whether an association between stuttering and alcohol consumption or regular smoking exists in late adolescence and adulthood. Regression analyses were carried out on data from a birth cohort study, the National Child Development Study (NCDS), whose initial cohort included 18,558 participants who have since been followed up until age 55. In the analyses, the main predictor variable was parent-reported stuttering at age 16. Parental socio-economic group, cohort member's sex and childhood behavioural problems were also included. The outcome variables related to alcohol consumption and smoking habits at ages 16, 23, 33, 41, 46, 50 and 55. No significant association was found between stuttering and alcohol consumption or stuttering and smoking at any of the ages. It was speculated that the absence of significant associations might be due to avoidance of social situations on the part of many of the participants who stutter, or adoption of alternative coping strategies. Because of the association between anxiety and substance use, individuals who stutter and are anxious might be found to drink or smoke excessively, but as a group, people who stutter are not more likely than those who do not to have high levels of consumption of alcohol or nicotine. Copyright © 2016 Elsevier Inc. All rights reserved.

  13. Energy consumption and technological developments

    International Nuclear Information System (INIS)

    Okorokov, V.R.

    1990-02-01

    The paper determines an outline of the world energy prospects based on principal trends of the development of energy consumption analysed over the long past period. According to the author's conclusion the development of energy systems will be determined in the nearest future (30 - 40 years) by contemporary energy technologies based on the exploitation of traditional energy resources but in the far future technologies based on the exploitation of thermonuclear and solar energy will play the decisive role. (author)

  14. Precision spectroscopy of high rotational states in H2 investigated by Doppler-free two-photon laser spectroscopy in the EF 1Σg+-X 1Σg+ system

    Science.gov (United States)

    Dickenson, G. D.; Salumbides, E. J.; Niu, M.; Jungen, Ch.; Ross, S. C.; Ubachs, W.

    2012-09-01

    Recently a high precision spectroscopic investigation of the EF1Σg+-X1Σg+ system of molecular hydrogen was reported yielding information on QED and relativistic effects in a sequence of rotational quantum states in the X1Σg+ ground state of the H2 molecule [Salumbides , Phys. Rev. Lett.PRLTAO0031-900710.1103/PhysRevLett.107.043005 107, 043005 (2011)]. The present paper presents a more detailed description of the methods and results. Furthermore, the paper serves as a stepping stone towards a continuation of the previous study by extending the known level structure of the EF1Σg+ state to highly excited rovibrational levels through Doppler-free two-photon spectroscopy. Based on combination differences between vibrational levels in the ground state, and between three rotational branches (O, Q, and S branches) assignments of excited EF1Σg+ levels, involving high vibrational and rotational quantum numbers, can be unambiguously made. For the higher EF1Σg+ levels, where no combination differences are available, calculations were performed using the multichannel quantum defect method, for a broad class of vibrational and rotational levels up to J=19. These predictions were used for assigning high-J EF levels and are found to be accurate within 5 cm-1.

  15. Avaliação de fenda marginal, através de microscopia eletrônica de varredura, em restaurações de resina composta com diferentes técnicas adesivas = Scanning electron microscopy evaluation of the marginal gap in composite restorations with different adhesive techniques

    Directory of Open Access Journals (Sweden)

    Coelho-de-Souza, Fábio Herrmann

    2008-01-01

    Full Text Available Objetivo: O propósito deste trabalho foi avaliar a formação de fenda marginal, através de microscopia eletrônica de varredura, em restaurações de resina composta com quatro técnicas adesivas: Prime & Bond 2. 1 (Dentsply sem condicionamento prévio, Prime & Bond 2. 1 com condicionamento convencional com ácido fosfórico, Prime & Bond 2. 1 com condicionamento com ácido poliacrílico a 25% e Clearfil SE Bond (Kuraray. Metodologia: Foram utilizados cinco terceiros molares humanos hígidos, nos quais foram preparadas quatro cavidades, sendo que em cada uma foi utilizada uma técnica adesiva. Todas as cavidades foram restauradas com resina composta Z250 (3M/ESPE. Os dentes foram submetidos à termociclagem por 500 ciclos e observados em microscópio eletrônico de varredura para medição da formação de fenda marginal nas paredes gengivais. Resultados: Não houve diferença estatística de fenda marginal entre os grupos experimentais de técnicas adesivas (ANOVA, P>0,05. Conclusão: Nenhuma das técnicas adesivas foi capaz de evitar a formação de fenda marginal; não houve diferença entre as técnicas adesivas empregadas

  16. Household vehicles energy consumption 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-08-01

    Household Vehicles Energy Consumption 1994 reports on the results of the 1994 Residential Transportation Energy Consumption Survey (RTECS). The RTECS is a national sample survey that has been conducted every 3 years since 1985. For the 1994 survey, more than 3,000 households that own or use some 6,000 vehicles provided information to describe vehicle stock, vehicle-miles traveled, energy end-use consumption, and energy expenditures for personal vehicles. The survey results represent the characteristics of the 84.9 million households that used or had access to vehicles in 1994 nationwide. (An additional 12 million households neither owned or had access to vehicles during the survey year.) To be included in then RTECS survey, vehicles must be either owned or used by household members on a regular basis for personal transportation, or owned by a company rather than a household, but kept at home, regularly available for the use of household members. Most vehicles included in the RTECS are classified as {open_quotes}light-duty vehicles{close_quotes} (weighing less than 8,500 pounds). However, the RTECS also includes a very small number of {open_quotes}other{close_quotes} vehicles, such as motor homes and larger trucks that are available for personal use.

  17. Energy sustainability: consumption, efficiency, and ...

    Science.gov (United States)

    One of the critical challenges in achieving sustainability is finding a way to meet the energy consumption needs of a growing population in the face of increasing economic prosperity and finite resources. According to ecological footprint computations, the global resource consumption began exceeding planetary supply in 1977 and by 2030, global energy demand, population, and gross domestic product are projected to greatly increase over 1977 levels. With the aim of finding sustainable energy solutions, we present a simple yet rigorous procedure for assessing and counterbalancing the relationship between energy demand, environmental impact, population, GDP, and energy efficiency. Our analyses indicated that infeasible increases in energy efficiency (over 100 %) would be required by 2030 to return to 1977 environmental impact levels and annual reductions (2 and 3 %) in energy demand resulted in physical, yet impractical requirements; hence, a combination of policy and technology approaches is needed to tackle this critical challenge. This work emphasizes the difficulty in moving toward energy sustainability and helps to frame possible solutions useful for policy and management. Based on projected energy consumption, environmental impact, human population, gross domestic product (GDP), and energy efficiency, for this study, we explore the increase in energy-use efficiency and the decrease in energy use intensity required to achieve sustainable environmental impact le

  18. Improvement of sunflower for consumption

    Directory of Open Access Journals (Sweden)

    Dijanović Dijana

    2003-01-01

    Full Text Available Growing sunflower (Heliianthus annuus L for consumption is becoming more and more attractive in the whole world and in our country, as well. The consumption of this product has been considerably increased because it is being used more and more in various forms: sunflower seed in a hull, hulled kernels fried or non-fried, salted or non-salted with addition of various spices. The sunflower kernel is used for preparing over 100 different food products: special types of bread, cakes, ice-cream, chocolate. In accordance with the trade demands, the aims of sunflower seeds for consumption improvement are: high productivity, greater content of proteins, lesser content of oil, oncreased mass of 1000 seeds, lesser portion of the hull easy nibbling, as well as tolerance to dominant diseases in the growing region. In the Agricultural and Technological Research Center in Zajecar, 4 genotypes of protein sunflower (two cultivars and two hybrids have been created so far. This study shows the results achieved in increasing the content of protein, the mass of 1000 seeds, decreasing the content of oil and hull of the new 90 hybrid combination. The hybrid combinations were obtained by crossing of the CMS and restorer lines.

  19. [Why is bread consumption decreasing?].

    Science.gov (United States)

    Rolland, M F; Chabert, C; Serville, Y

    1977-01-01

    In France bread plays a very special and ambivalent role among our foodstuffs because of the considerable drop in its consumption, its alleged harmful effects on our health and the respect in which it is traditionally held. More than half the 1 089 adults interviewed in this study say they have decreased their consumption of bread in the last 10 years. The reasons given vary according to age, body weight and urbanization level. The main reasons given for this restriction are the desire to prevent or reduce obesity, the decrease in physical activity, the general reduction in food consumption and the possibility of diversifying foods even further. Moreover, the decreasing appeal of bread in relation to other foods, as well as a modification in the structure of meals, in which bread becomes less useful to accompany other food, accentuate this loss of attraction. However, the respect for bread as part of the staple diet remains very acute as 95 p. 100 of those interviewed express a reluctance to throw bread away, more for cultural than economic reasons. Mechanization and urbanization having brought about a decrease in energy needs, the most common alimentary adaptation is general caloric restriction by which glucids, and especially bread, are curtailed.

  20. Passenger car fuel consumption survey

    Energy Technology Data Exchange (ETDEWEB)

    1984-03-01

    This survey originated from a proposal to monitor the fuel consumption and fuel economy of personal use passenger cars operated in Canada. Its purpose is to establish a data base which would contain information on total distance travelled, total amount of fuel consumed, average distance obtained per unit of fuel, total expenditures on fuel, and seasonal fluctuations in fuel consumption and in distance travelled. Among the needs served by this data base are the monitoring of passenger car fuel economy standards and the estimation of pasenger car fuel requirements in conditions involving fuel shortages. Survey methodology is by telephone interview to trace selected vehicles to the registered owners, at which time a fuel purchase diary is then mailed to the principal driver of the car. The results are tabulated on a quarterly basis and to be released as they become available in bulletins similar to this. Data are presented for each province and the total for Canada is given. During the fourth quarter of 1982, it is estimated that there were 7.3 million personal use passenger cars operated in Canada. These cars were driven 28 billion kilometers and consumed 4.3 billion litres of fuel. Their average litres/100 kilometres and the average fuel consumption was 590 litres. 8 tabs.

  1. The mazEF toxin–antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis

    Directory of Open Access Journals (Sweden)

    Soheili S

    2015-05-01

    Full Text Available Sara Soheili,1 Sobhan Ghafourian,2 Zamberi Sekawi,1 Vasantha Kumari Neela,1 Nourkhoda Sadeghifard,2 Morovat Taherikalani,2 Afra Khosravi,2 Ramliza Ramli,3 Rukman Awang Hamat11Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health sciences, Universiti Putra Malaysia, Serdang, Malaysia; 2Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran; 3Department of Medical Microbiology and Immunology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaakob Latif, Bandar Tun Razak, Kuala Lumpur, Malaysia Abstract: The toxin–antitoxin (TA system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. In this study, the prevalence of TA systems in independently isolated clinical isolates of Enterococcus faecium and Enterococcus faecalis was determined, the dominant TA system was identified, different virulence genes in E. faecium and E. faecalis were surveyed, the level of expression of the virulence and TA genes in normal and stress conditions was determined, and finally their associations with the TA genes were defined. Remarkably, the analysis demonstrated higBA and mazEF in all clinical isolates, and their locations were on chromosomes and plasmids, respectively. On the other hand, a quantitative analysis of TA and virulence genes revealed that the expression level in both genes is different under normal and stress conditions. The results obtained by anti-mazF peptide nucleic acids demonstrated that the expression level of virulence genes had decreased. These findings demonstrate an association between TA systems and virulence factors. The mazEF on the plasmids and the higBA TA genes on the chromosomes of all E. faecium and E. faecalis strains were dominant. Additionally, there was a decrease in the expression of virulence genes in the presence of anti-mazF peptide nucleic acids. Therefore, it is suggested that mazEF TA systems

  2. Self-consumption. German experience feedback

    International Nuclear Information System (INIS)

    Roesner, Sven

    2015-01-01

    This document presents, first, the definition and context of self-consumption (photovoltaic and cogeneration), then its profitability (regulation and financial incentive), next, the direct consumption by third parties, and finally, the opportunities and technical challenges

  3. Factors that negatively influence consumption of traditionally ...

    African Journals Online (AJOL)

    Factors that negatively influence consumption of traditionally fermented milk ... in various countries of sub-Saharan Africa and a number of health benefits to human ... influence consumption of Mursik, a traditionally fermented milk product from ...

  4. Energetic consumption levels and human development indexes

    International Nuclear Information System (INIS)

    Boa Nova, Antonio Carlos

    1999-01-01

    The article overviews the energetic consumption levels and human development indexes. The human development indexes are described based on the United Nations Development Programme. A comparison between the energetic consumption levels and human development indexes is also presented

  5. Impact of Exhaled Breath Acetone in the Prognosis of Patients with Heart Failure with Reduced Ejection Fraction (HFrEF. One Year of Clinical Follow-up.

    Directory of Open Access Journals (Sweden)

    Fabiana G Marcondes-Braga

    Full Text Available The identification of new biomarkers of heart failure (HF could help in its treatment. Previously, our group studied 89 patients with HF and showed that exhaled breath acetone (EBA is a new noninvasive biomarker of HF diagnosis. However, there is no data about the relevance of EBA as a biomarker of prognosis.To evaluate whether EBA could give prognostic information in patients with heart failure with reduced ejection fraction (HFrEF.After breath collection and analysis by gas chromatography-mass spectrometry and by spectrophotometry, the 89 patients referred before were followed by one year. Study physicians, blind to the results of cardiac biomarker testing, ascertained vital status of each study participant at 12 months.The composite endpoint death and heart transplantation (HT were observed in 35 patients (39.3%: 29 patients (32.6% died and 6 (6.7% were submitted to HT within 12 months after study enrollment. High levels of EBA (≥3.7μg/L, 50th percentile were associated with a progressively worse prognosis in 12-month follow-up (log-rank = 11.06, p = 0.001. Concentrations of EBA above 3.7μg/L increased the risk of death or HT in 3.26 times (HR = 3.26, 95%CI = 1.56-6.80, p = 0.002 within 12 months. In a multivariable cox regression model, the independent predictors of all-cause mortality were systolic blood pressure, respiratory rate and EBA levels.High EBA levels could be associated to poor prognosis in HFrEF patients.

  6. Impact of Exhaled Breath Acetone in the Prognosis of Patients with Heart Failure with Reduced Ejection Fraction (HFrEF). One Year of Clinical Follow-up

    Science.gov (United States)

    Saldiva, Paulo H. N.; Mangini, Sandrigo; Issa, Victor S.; Ayub-Ferreira, Silvia M.; Bocchi, Edimar A.

    2016-01-01

    Background The identification of new biomarkers of heart failure (HF) could help in its treatment. Previously, our group studied 89 patients with HF and showed that exhaled breath acetone (EBA) is a new noninvasive biomarker of HF diagnosis. However, there is no data about the relevance of EBA as a biomarker of prognosis. Objectives To evaluate whether EBA could give prognostic information in patients with heart failure with reduced ejection fraction (HFrEF). Methods After breath collection and analysis by gas chromatography-mass spectrometry and by spectrophotometry, the 89 patients referred before were followed by one year. Study physicians, blind to the results of cardiac biomarker testing, ascertained vital status of each study participant at 12 months. Results The composite endpoint death and heart transplantation (HT) were observed in 35 patients (39.3%): 29 patients (32.6%) died and 6 (6.7%) were submitted to HT within 12 months after study enrollment. High levels of EBA (≥3.7μg/L, 50th percentile) were associated with a progressively worse prognosis in 12-month follow-up (log-rank = 11.06, p = 0.001). Concentrations of EBA above 3.7μg/L increased the risk of death or HT in 3.26 times (HR = 3.26, 95%CI = 1.56–6.80, p = 0.002) within 12 months. In a multivariable cox regression model, the independent predictors of all-cause mortality were systolic blood pressure, respiratory rate and EBA levels. Conclusions High EBA levels could be associated to poor prognosis in HFrEF patients. PMID:28030609

  7. State energy data report 1992: Consumption estimates

    Energy Technology Data Exchange (ETDEWEB)

    1994-05-01

    This is a report of energy consumption by state for the years 1960 to 1992. The report contains summaries of energy consumption for the US and by state, consumption by source, comparisons to other energy use reports, consumption by energy use sector, and describes the estimation methodologies used in the preparation of the report. Some years are not listed specifically although they are included in the summary of data.

  8. Household energy and consumption and expenditures, 1990

    International Nuclear Information System (INIS)

    1993-01-01

    The purpose of this supplement to the Household Energy Consumption and Expenditures 1990 report is to provide information on the use of energy in residential housing units, specifically at the four Census regions and nine Census division levels. This report includes household energy consumption, expenditures, and prices for natural gas, electricity, fuel oil, liquefied petroleum gas (LPG), and kerosene as well as household wood consumption. For national-level data, see the main report, Household Energy Consumption and Expenditures 1990

  9. Optimal Smooth Consumption and Annuity Design

    DEFF Research Database (Denmark)

    Bruhn, Kenneth; Steffensen, Mogens

    2013-01-01

    We propose an optimization criterion that yields extraordinary consumption smoothing compared to the well known results of the life-cycle model. Under this criterion we solve the related consumption and investment optimization problem faced by individuals with preferences for intertemporal stabil...... stability in consumption. We find that the consumption and investment patterns demanded under the optimization criterion is in general offered as annuity benefits from products in the class of ‘Formula Based Smoothed Investment-Linked Annuities’....

  10. Power Consumption of a MOSFET

    Directory of Open Access Journals (Sweden)

    Frederick Selkey

    2010-01-01

    Full Text Available A MOSFET is defined as metal oxide semiconductor field effect transistor. These electrical components are combined or integrated to form control and logic functions for laptop and desktop computers, power controls in printing devices, motor controls and are used in many other electrical circuits. All electrical devices consume electrical power based on current and voltage. For this paper we calculated the power consumption of a Toshiba 2SK3563 MOSFET during its triode mode by finding the area under the current-voltage characteristic curve.

  11. Taxation and Consumption of Wine

    OpenAIRE

    Tsolakis, Dimitris

    1983-01-01

    In this article, the impact that a sales tax might have upon wine consumption and, hence, on the wine and grape growing industries, is analysed. Implications for government revenue are also considered. It is shown that the relative responsiveness of supply and demand, rather than the level in the marketing chain at which a tax is levied, determines where the tax burden finally falls. The imposition of a tax on wine might force certain sectors of the wine and grape industries to undergo a phas...

  12. Heavy consumption and drink driving

    DEFF Research Database (Denmark)

    Fynbo, Lars

    2010-01-01

    This paper is part of an ongoing mixed methods project about untreated heavy alcohol consumption amongst adult Danes. It is based upon 21 in-depth qualitative interviews with convicted drink drivers. All interviewees were contacted while attending mandatory courses in “Alcohol and Traffic safety...... on the interviewee’s risk behaviour, especially in relation to driving. The interviewees are first divided into 1) a group of young “edgeworkers” with pronounced general risk behaviour, 2) a group of middle-aged “post-edgeworkers”, most with criminal records, and 3) a group of middle-aged and older heavy consumers...

  13. Microscopia eletrônica de varredura do endosperma de café (Coffea arabica L. durante o processo de secagem Scanning electron microscopy of the endosperm of coffee (Coffea arabica L. during the drying process

    Directory of Open Access Journals (Sweden)

    Reni Saath

    2010-02-01

    Full Text Available A manutenção da integridade das membranas celulares, entre outros eventos, é um forte indicativo de que a qualidade do café foi preservada na pós-colheita. Objetivou-se neste trabalho, analisar o efeito de diferentes métodos de secagem na manutenção da integridade da parede celular e da membrana plasmática de café natural e café despolpado, buscando determinar as condições e o momento em que ocorrem as rupturas microscópicas. Os cafés foram submetidos a um período de pré-secagem em terreiro. Após este, uma parcela de cada tipo de café foi desidratada no terreiro e, outra, à temperatura de 40ºC e 60ºC em secadores de camada fixa, monitorando-se a temperatura e o teor de água até 11% (bu. Nesse período, grãos foram aleatoriamente amostrados e fragmentos do endosperma preparados para a microscopia eletrônica de varredura, registrando-se diversas eletromicrografias, avaliando-se as alterações na membrana plasmática da célula do endosperma dos grãos de cafés em função do teor de água e tempo de secagem. O citoplasma das células a 11% (bu de teor de água não foi comprometido na secagem em terreiro e a 40°C; na secagem a 60°C, observou-se comprometimento nas estruturas celulares nos cafés com teor de água de 20% (bu.The maintenance of the integrity of cellular membranes, among other events, is a strong indicator that the quality of the coffee was preserved in the post-harvesting process. Therefore, this work aimed to analyze the effect of different drying methods on the maintenance of the integrity of cell walls and plasma membrane of natural and de-pulped coffee in order to determine the conditions and the moment that microscopic ruptures take place. The coffee was submitted to a pre-drying period on a concrete patio. After this, a sample of each type of coffee was dehydrated outdoors and another, with heated air at 40ºC and 60ºC in fixed-layer dryers, controlling the grain temperature and the moisture

  14. Diagnóstico cefalométrico eletrônico: contextualização de variáveis cefalométricas Electronic cephalometric diagnose: contextualized cephalometric variables

    Directory of Open Access Journals (Sweden)

    Marinho Del Santo Jr

    2011-04-01

    Full Text Available INTRODUÇÃO: avaliações paramétricas clássicas e variáveis cefalométricas isoladas podem não prover a melhor informação em morfologia craniofacial. Por outro lado, uma cefalometria contextualizada pode ser bem mais promissora, uma vez que permite a integração de variáveis cefalométricas ponderadas. OBJETIVO: a proposta principal desse artigo é apresentar a aplicação de um modelo matemático não-trivial em cefalometria, permitindo a mineração de dados através da filtragem de certeza e contradição em cada "nó" da rede. MÉTODOS: nessa "rede neural" proposta, cada "célula" é conectada a outras "células" através de "sinapses". Tal sistema de tomada de decisão é uma ferramenta de inteligência artificial ajustada para potencialmente aumentar o significado dos dados coletados. RESULTADOS: a comparação entre o diagnóstico final fornecido pela rede neural paraconsistente e as opiniões de três examinadores foi heterogênea. O índice de concordância Kappa foi regular para discrepâncias anteroposteriores; substancial ou regular para discrepâncias verticais; e moderado para discrepâncias dentárias. Para a protrusão bimaxilar dentária, a concordância foi quase perfeita. Similarmente, a concordância entre as opiniões dos três examinadores sem a ajuda de nenhuma ferramenta de diagnóstico foi apenas moderada para discrepâncias esqueletais e dentárias. Como exceção, a concordância para protrusão dentária foi quase perfeita. CONCLUSÕES: a avaliação de desempenho do sistema tecnológico desenvolvido suporta que a ferramenta eletrônica apresentada pode se igualar às decisões humanas na maioria das situações. Como uma limitação esperada, tal ferramenta matemática-computacional se apresentou menos efetiva para discrepâncias esqueletais do que para discrepâncias dentárias.INTRODUCTION: Classical parametric assessments and isolated cephalometric variables may not provide the best information in

  15. I Workshop Virtual Cibereduc: SEER: Periódicos eletrônicos: Editoração e acesso/I Workshop Virtual Cibereduc: SEER: Electronic journals – Editing and access

    Directory of Open Access Journals (Sweden)

    Suely de Brito Clemente Soares

    2004-01-01

    Full Text Available Descrição do “I Workshop Virtual CiberEduc”, ocorrido no dia 14.10.2004, das 9:00 às 11:00 horas. Foram conectadas duas salas de videoconferência, uma na Faculdade de Educação da UNICAMP, em Campinas, SP, e outra na RNP, Brasília, com retransmissão simultânea via Internet, o que permitiu aos participantes uma interação síncrona, mediante envio de perguntas para o e-mail ciberedu@unicamp.br. O tema discutido foi “SEER: periódicos eletrônicos: editoração e acesso”. As inscrições, gratuitas, foram feitas on-line. Os certificados ficaram disponíveis posteriormente, em formato PDF, para impressão, aos inscritos que comprovaram participação enviando, por e-mail, pelo menos uma pergunta sobre o SEER. Foram recebidas duzentos e dezoito inscrições e expedidos cento e vinte e seis certificados. Foram respondidas trinta e cinco perguntas durante o workshop e dez posteriormente, por e-mail. Dois servidores web da UNICAMP retransmitiram o evento. Instruções foram fornecidas antecipadamente aos inscritos, um telefone para contato durante o evento foi divulgado e, mesmo assim, nem todos os inscritos conseguiram conexão em tempo real. O vídeo do evento e os arquivos das apresentações ficaram disponíveis na Internet para consulta posterior. A experiência foi positiva, tanto na opinião dos organizadores, dos conferencistas, como dos inscritos, sendo um estímulo para a continuidade desses workshops virtuais. Description of the virtual event “I Workshop Virtual CiberEduc”, which had placed on the 14th of September 2004, from 9am to 11am. Two videoconference rooms were connected; one at the Faculdade de Educação da UNICAMP, in Campinas, São Paulo, and other at the Rede Nacional de Pesquisa in Brasília, with transmission on-line on the Internet, this fact allowed the synchronic interaction trough the use of e-mail for submission of questions to ciberedu@unicamp.br. Two Web servers of the UNICAMP were used to transmit

  16. Electricity consumption in Geneva's businesses and administration

    International Nuclear Information System (INIS)

    Bassand, M.; Malatesta, D.; Chevalier, H.

    1999-01-01

    The article describes a data-collection project that gave an overview of energy consumption in Geneva's businesses and the canton's administration. For the first time, data on the patterns of use of electrical equipment were collected. The development of the sampling process is discussed, and the development of the categories defined for the analysis of the results is described. The role of various types of objects and their typical electricity consumption is discussed and trends in consumption are described. Results discussed include electricity consumption per employee and the degree of awareness regarding costs and consumption in various business types

  17. Household energy consumption and expenditures, 1990

    International Nuclear Information System (INIS)

    1993-01-01

    This report, Household Energy Consumption and Expenditures 1990, is based upon data from the 1990 Residential Energy Consumption Survey (RECS). Focusing on energy end-use consumption and expenditures of households, the 1990 RECS is the eighth in a series conducted since 1978 by the Energy Information Administration (EIA). Over 5,000 households were surveyed, providing information on their housing units, housing characteristics, energy consumption and expenditures, stock of energy-consuming appliances, and energy-related behavior. The information provided represents the characteristics and energy consumption of 94 million households nationwide

  18. Life-Cycle Consumption and Children

    DEFF Research Database (Denmark)

    Jørgensen, Thomas Høgholm

    I show that conventional estimators based on the consumption Euler equation, extensively used in studies of intertemporal consumption behavior, produce inconsistent estimates of the effect of children on consumption if potentially binding credit constraints are ignored. As a more constructive...... contribution, I supply a tractable approach to obtaining bounds on the effect of children and a structural estimation strategy when households face constraints. Finally, I estimate the effect of children on consumption using the Panel Study of Income Dynamics (PSID) for the US and high quality Danish...... administrative register data. Results suggest that children does not affect household consumption in the same magnitude previously assumed....

  19. Socio-Demographic Differences in Energy Drink Consumption and Reasons for Consumption among US College Students

    Science.gov (United States)

    Poulos, Natalie S.; Pasch, Keryn E.

    2016-01-01

    Background: Energy drink consumption has become increasingly prevalent among US college students, yet little is known about current rates of consumption and reasons for consumption among current energy drink users, particularly differences related to gender and race/ethnicity. Objectives: To better understand energy drink consumption alone and…

  20. Resource consumption, sustainability, and cancer.

    Science.gov (United States)

    Kareva, Irina; Morin, Benjamin; Castillo-Chavez, Carlos

    2015-02-01

    Preserving a system's viability in the presence of diversity erosion is critical if the goal is to sustainably support biodiversity. Reduction in population heterogeneity, whether inter- or intraspecies, may increase population fragility, either decreasing its ability to adapt effectively to environmental changes or facilitating the survival and success of ordinarily rare phenotypes. The latter may result in over-representation of individuals who may participate in resource utilization patterns that can lead to over-exploitation, exhaustion, and, ultimately, collapse of both the resource and the population that depends on it. Here, we aim to identify regimes that can signal whether a consumer-resource system is capable of supporting viable degrees of heterogeneity. The framework used here is an expansion of a previously introduced consumer-resource type system of a population of individuals classified by their resource consumption. Application of the Reduction Theorem to the system enables us to evaluate the health of the system through tracking both the mean value of the parameter of resource (over)consumption, and the population variance, as both change over time. The article concludes with a discussion that highlights applicability of the proposed system to investigation of systems that are affected by particularly devastating overly adapted populations, namely cancerous cells. Potential intervention approaches for system management are discussed in the context of cancer therapies.