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Sample records for confer ouabain sensitivity

  1. Effect of low level laser therapy (LLLT) on ouabain induced auditory neuropathy in gerbils (Conference Presentation)

    Science.gov (United States)

    Rhee, Chung-Ku; Bae, Sung Huyn; Chang, So-Young; Chung, Phil-Sang; Jung, Jae-Yun

    2016-02-01

    Aim: to investigate effectiveness of Low level laser therapy (LLLT) in rescueing ouabain induced spiral ganglion cell damage using Mongolian gerbils. Methods: Animals were divided into 3 groups; Control, Ouabain, Ouabain + LLLT group. Auditory neuropathy was induced by topical application of ouabain (1 mmol/L, 3uL) on the round window membrane in gerbils. Transmeatal LLLT was irradiated into the right ear for 1h (200mW, 720 J) daily for 7d in Ouabain + LLLT group. Before and 7 days after ouabain application, hearing was evaluated using both ABR and distortion product otoacoustic emissions (DPOAE). Seven days after ouabain application, animals were sacrificed to evaluate the morphological changes of cochlea using cochlear section image and whole mount Immunofluorescent staining. Results: DPOAE tests were normal in all animals after ouabain topical treatment indicating intact outer hair cells. Ouabain group showed ABR threshold increase compared with control group. Ouabain+LLLT group showed significant improvement of ABR threshold compared to ouabain only group. H and E stains of mid-modiolar section of cochlear showed spiral ganglion cells, neurofilaments, and post synaptic receptor counts were decreased while inner and outer hair cells were preserved in ouabain group. Ouabain +LLLT group showed higher numbers of spiral ganglion cells, density of neurofilaments and post synaptic receptor counts compared to ouabain group. Conclusions: The results demonstrated that LLLT was effective to rescue ouabain-induced spiral ganglion neuropathy.

  2. The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain

    DEFF Research Database (Denmark)

    Matchkov, Vladimir; Nilsson, Holger; Aalkjær, Christian

    Ouabain, an inhibitor of the Na+-pump, has been shown to inhibit intercellular communication. We have recently shown that gap junctions between vascular smooth muscle cells (SMCs) are regulated through an interaction between a ouabain-sensitive isoform of the Na+-pump and the Na+/Ca2+-exchanger...... electrical coupling was evaluated in functional studies. SMCs were electrically uncoupled when the ouabain-sensitive Na+-pump was inhibited by 10 mM ouabain. Inhibition of the Na+/Ca2+-exchanger with 1 mM SEA0400 also uncoupled the SMCs. Depletion of [Na+]i and clamping [Ca2+]i at low levels prevented......+-exchanger-1 and connexin-43. The α3 Na+-pump subunit was not associated with these proteins but co-immunoprecipitated with caveolin-1. Based on these experiments we suggest that α2 Na+ -pump subunit is involved in regulation of the intercellular communication via interaction with the Na+/Ca2+-exchanger-1...

  3. The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain

    DEFF Research Database (Denmark)

    Matchkov, Vladimir; Nilsson, Holger; Aalkjær, Christian

    leading to increases in [Ca2+]i in discrete areas near the plasma membrane. This suggests close association of these transport proteins in microdomains. Using PCR and co-immunoprecipitation we aimed to test this hypothesis in SMCs from mesenteric small arteries and in A7r5 cell line. Intercellular...... electrical coupling was evaluated in functional studies. SMCs were electrically uncoupled when the ouabain-sensitive Na+-pump was inhibited by 10 mM ouabain. Inhibition of the Na+/Ca2+-exchanger with 1 mM SEA0400 also uncoupled the SMCs. Depletion of [Na+]i and clamping [Ca2+]i at low levels prevented...... leading to local [Ca2+]i transients near the membrane which block the closely associated connexin-43 containing gap junctions....

  4. Ouabain sensitive Na+/K+-pump regulates other membrane transporters in the microdomain of smooth muscle cells

    DEFF Research Database (Denmark)

    Matchkov, Vladimir; Nilsson, Holger; Aalkjær, Christian

    Ouabain, a specific inhibitor of the Na+/K+-pump, has previously been shown to interfere with intercellular communication. We have demonstrated a mechanism of this action of ouabain (1). We have showed that gap junctions between vascular smooth muscle cells (SMCs) are regulated through......+/K+-pump-containing microdomain is functionally linked to KATP channels via the local ion homeostasis and that this interaction can be bidirectional (1;2). [Ca2+]i in individual SMCs was imaged simultaneously with isometric force in rat mesenteric small arteries. Paired cultured rat aortic smooth muscle cells (A7r5) were used...

  5. Relationship between ouabain and asthenozoospermia.

    Science.gov (United States)

    Yang, Yi-hong; Wan, Yan; Lou, Huan; Xue, Ting; Su, Ping

    2014-02-01

    A growing number of researches have shown that ouabain can regulate mammalian sperm function and male reproduction by modulating the sperm motility, capacitation and acrosome reaction in vitro. This study further examined the relationship between ouabain and asthenozoospermia. In this study, the rat was intraperitoneally injected with ouabain at different concentrations (low-dose ouabain group: 12.5 μg/kg body weight per day, and high-dose ouabain group: 25 μg/kg body weight per day) for 30 days to establish the asthenozoospermia model. The sperms from 60 males with normal fertility were incubated with ouabain of gradient concentrations (10(-7)-10(-2) mol/L) for 4 h. The sperm motility was evaluated under a microscope. Moreover, the endogenous ouabain (EO) level was determined in seminal plasma of mild or severe asthenozoospermia patients and males with normal fertility by competitive inhibition ELISA. The results showed that the sperm motility was significantly diminished in the rats treated with different concentrations of ouabain. The number of motile sperms (grades a and b) was decreased greatly in a time- and dose-dependent manner in 10(-5)-10(-2) mol/L ouabain groups (P0.05). Furthermore, the EO level was significantly increased in asthenozoospermia patients as compared with that in males with normal fertility (25.27±1.71 μg/L in mild asthenozoospermia patients, 26.52±1.82 μg/L in severe asthenozoospermia patients, 19.31±1.45 μg/L in normal fertility men) (Pasthenozoospermia was successfully established by intraperitoneal injection of ouabain, and 10(-5) mol/L ouabain was sufficient enough to inhibit sperm motility in vitro. Moreover, EO, a normal constituent of seminal plasma, was highly expressed in asthenozoospermia males as compared with normal fertility ones.

  6. Structure-function relationships in the Na,K-ATPase. cap alpha. subunit: site-directed mutagenesis of glutamine-111 to arginine and asparagine-122 to aspartic acid generates a ouabain-resistant enzyme

    Energy Technology Data Exchange (ETDEWEB)

    Price, E.M.; Lingrel, J.B.

    1988-11-01

    Na,K-ATPases from various species differ greatly in their sensitivity to cardiac glycosides such as ouabain. The sheep and human enzymes are a thousand times more sensitive than the corresponding ones from rat and mouse. To define the region of the ..cap alpha..1 subunit responsible for this differential sensitivity, chimeric cDNAs of sheep and rat were constructed and expressed in ouabain-sensitive HeLa cells. The construct containing the amino-terminal half of the rat ..cap alpha..1 subunit coding region and carboxyl-terminal half of the sheep conferred the ouabain-resistant phenotype to HeLa cells while the reverse construct did not. This indicates that the determinants involved in ouabain sensitivity are located in the amino-terminal half of the Na,K-ATPase ..cap alpha.. subunit. By use of site-directed mutagenesis, the amino acid sequence of the first extracellular domain (H1-H2) of the sheep ..cap alpha..1 subunit was changed to that of the rat. When expressed in HeLa cells, this mutated sheep ..cap alpha..1 construct, like the rat/sheep chimera, was able to confer ouabain resistance to these cells. Furthermore, similar results were observed when HeLa cells were transfected with a sheep ..cap alpha..1 cDNA containing only two amino acid substitutions. The resistant cells, whether transfected with the rat ..cap alpha..1 cDNA, the rat/sheep chimera, or the mutant sheep ..cap alpha..1 cDNAs, exhibited identical biochemical characteristics including ouabain-inhibitable cell growth, /sup 86/Rb/sup +/ uptake, and Na,K-ATPase activity. These results demonstrate that the presence of arginine and aspartic acid on the amino end and carboxyl end, respectively, of the H1-H2 extracellular domain of the Na,K-ATPase ..cap alpha.. subunit together is responsible for the ouabain-resistant character of the rat enzyme and the corresponding residues in the sheep ..cap alpha..1 subunit (glutamine and asparagine) are somehow involved in ouabain binding.

  7. Influence of lidocaine on ouabain-induced inotropic response in rat atria.

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    Sterin-Borda, Leonor; Orman, Betina; Reina, Silvia; Borda, Enri

    2003-11-01

    In this paper we demonstrated that lidocaine broadens the therapeutic range of ouabain action having a protective effect on ouabain-induced toxicity on rat atria. The lidocaine effect on therapeutic ouabain action was associated with the increase in the sensitivity of Na(+)-K(+)-ATPase related to a decreased in the equilibrium dissociation constant (K(d)) of high affinity binding sites. Lidocaine suppressed the ouabain-induced tonotropic effect and arrhythmias, decreasing the number of low affinity binding sites (B(max)) without changes in K(d). Blockade of Na(+)-Ca(2+) exchange with KB-R7943 or dual Na(+)-Ca(2+) channel with flunarizine, mimicked lidocaine effect increasing ouabain therapeutic action, extending its concentration range tolerated, delaying the onset of contracture. Lidocaine itself triggered negative inotropic response at high concentration. This effect was increased in the presence of flunarizine and verapamil but not by the inhibition of calcium/calmodulin with W-7. The mechanism underlying the lidocaine-induced negative inotropic response, appears to be different that underlying the positive inotropic effect on ouabain action. This study provides evidence that lidocaine can interact with the same or similar binding sites for ouabain in rat atrial tissue, providing a protective effect on ouabain-induced changes in contractility. The contribution of Na(+)-Ca(2+) exchange and/or Ca(2+) overload on lidocaine effect is discussed.

  8. Two-phase positive inotropic effects of ouabain and the presence of multiple classes of ouabain binding sites in the ferret heart

    Energy Technology Data Exchange (ETDEWEB)

    Ng, Y.C.; Akera, T.

    1986-03-05

    Characteristics of more than one class of ouabain receptors which appear to exist in ferret heart were examined. In isolated papillary muscle, 1 to 30 nM ouabain produced a positive inotropic effect in the presence of 5 ..mu..M propranolol and 2 ..mu..M phentolamine. Higher concentrations of ouabain (0.1 to 10 ..mu..M) produced an additional and prominent inotropic effect. In partially purified Na, K-ATPase, ouabain caused a monophasic inhibition; however, the concentration-inhibition curve spanned over 5 log units, indicating that ouabain is interacting with more than a single class of the enzyme. Scatchard analysis of specific /sup 3/H-ouabain binding revealed approximately equal abundance of high and low affinity binding sites. The K/sub D/ value for high affinity sites was approximately 20 nM whereas that for low affinity sites was about 45 times higher. When phosphoenzyme was formed in the presence of (..gamma..-/sup 32/P)-ATP, Mg/sup 2 +/ and Na/sup +/ and subjected to SDS gel electrophoresis, two distinct K/sup +/-sensitive bands with about 100,000 dalton molecular weight were detected. Molecular weight difference between these two bands was approximately 2500 dalton. Phosphorylation of either band was abolished by 1 ..mu..M ouabain suggesting that both bands may correspond to the high-affinity binding sites. These results indicate that high and low affinity ouabain binding sites exists in approximately equal abundance in the ferret heart, and that binding of ouabain to these sites cases Na,K-ATPase inhibition and the positive inotropic effect.

  9. Ouabain inhibition of the sodium-potassium pump: estimation of ED50 in different types of human leucocytes in vitro

    DEFF Research Database (Denmark)

    Møller, B; Vaag, Allan; Johansen, Torben

    1990-01-01

    uptake of K+ (86Rb+) that is sensitive to ouabain, 10(-5) mol l-1. 2. Dose-response curves for inhibition of the Na(+)-K+ pump were obtained after exposure of the cells to various concentrations of ouabain for 2.5 h when the level of pump inhibition was considered to be at steady state. 3. The ED50...

  10. Endogenous Ouabain in Hypertensive Disorder Complicating Pregnancy

    Institute of Scientific and Technical Information of China (English)

    WU Li; XIONG Chengliang; SU Ping

    2007-01-01

    Expression of endogenous ouabain in placenta and the concentrations of serum ET-1 and NO were examined in 30 patients with hypertensive disorder complicating pregnancy (HDCP) and 30 healthy pregnant women to investigate the effect of endogenous ouabain on HDCP. Compared with the healthy pregnant group, the expression of endogenous ouabain dramatically increased in the HDCP groups (P<0.01). There was a significantly positive correlation between the expression of en- dogenous ouabain with ET-1 (r=0.5567, P<0.01), while the correlation of endogenous ouabain and NO was significantly negative (r=-0.6895, P<0.01). As expected, the correlation between ET-1 and NO was negative (r=-0.7796, P<0.01). ET-1 concentrations of maternal and cord sera in HDCP groups were significantly higher in comparison with healthy pregnant group (P<0.01). On the con- trast, NO concentrations were much lower in the maternal and cord sera of HDCP groups as com- pared with healthy pregnant group (P<0.01). Our data suggest that endogenous ouabain is directly involved in the nosogenesis of HDCP, with accompanying decreased NO and the elevated of ET-1.

  11. Comparative study of renal sodium transport between ouabain-hypertensive rats and ouabain-nonhypertensive rats

    Institute of Scientific and Technical Information of China (English)

    GE Heng; Lü Zhuo-ren

    2006-01-01

    Objective: To compare renal sodium transport, using fractional excretions of lithium(FEii)as a marker of proximal tubule sodium reabsorption, between hypertensive and non-hypertensive ouabaintreated rats and further to elucidate the role of ouabain in pathogenesis of hypertension. Methods:Thirty male Sprague-Dawley rats weighting 180-200 g were randomly divided into normal control group and ouabain treated group. Rats were infused with 1 ml/kg · d normal saline or 27.8 μg/kg · d ouabain intraperitoneally once a day respectively. Systolic blood pressure (SBP), heart rate and body weight were recorded weekly. Rats were sacrificed 6 weeks after treatment. Blood and 24-hour urine sample were collected to measure the serum and urinary concentration of sodium, trace lithium and creatinine. Endogenous creatinine clearance rate (Ccr), fractional excretions of sodium (FENa), fractional excretions of lithium (FELi) and fractional reabsorption of sodium in the postproximal tubules (FDRNa) were calculated.Ouabain levels of plasma and renal tissue, plasma renin activity, angiotensin Ⅱ and aldosterone concentration were determined. Results: 65% of the ouabain-treated rats achieved significantly higher SBP after 4weeks, compared with that of the saline control groups or self baseline (P<0. 01). But in the other 35%of the ouabain-treated rats, their SBP was similar with control group during the experiment (P>0. 05).The body weight, heart rate and food intake between the 3 groups were no significant differences (P>0.05). FELi and FDRNa were significantly lower in ouabain-hypertensive group compared with ouabain-nonhypertensive group and control group(P<0.01 and P<0.05). The FELi and FDRNa of ouabain-nonhypertensive groups were similar with control group(P>0.05). Ccr and FENa were comparable between the 3 groups (P>0. 05). Plasma and renal tissue ouabain levels, plasma renin activity, angiotensin Ⅱ and aldosterone contents in ouabain-hypertensive rats were

  12. Interaction of atrial natriuretic peptide and ouabain in the myocardium.

    Science.gov (United States)

    Nesher, Maoz; Bai, Yan; Li, Daxiang; Rosen, Haim; Lichtstein, David; Liu, Lijun

    2012-10-01

    Natriuretic peptides and digitalis-like compounds serve as regulators of homeostasis, including control of volume expansion and blood pressure. The aim of the present study was to explore possible interactions between atrial natriuretic peptide (ANP) and ouabain in the heart. ANP (1 nmol/L) had no effect in papillary muscle preparations from guinea pigs. Ouabain (1 µmol/L) induced positive inotropic effect. The addition of ANP prior to ouabain resulted in a significant decrease in the ouabain-induced positive inotropic effect, manifested as an attenuated increase in twitch maximal upward force slope and resting muscular tension. In addition, ANP caused an increase in Na⁺-K⁺-ATPase activity in heart microsomal preparations. The effect of ouabain on Na⁺-K⁺-ATPase activity was shown in a biphasic manner. Ouabain (0.01-1 nmol/L) had a small but significant increase on pump activity, but higher doses of ouabain inhibited activity. ANP attenuated ouabain-induced Na⁺-K⁺-ATPase activity. Furthermore, ouabain (50 nmol/L) or ANP (10 nmol/L) alone induced Akt activation in cardiomyocytes. However, ANP blocked ouabain-induced Akt activation. These results point to the existence of interactions between ANP and ouabain on Na⁺-K⁺-ATPase signaling and function in the heart, which may be mediated by regulation of Na⁺-K⁺-ATPase activity and (or) signal transduction mechanisms.

  13. The Influence of Ouabain on Human Dendritic Cells Maturation

    Directory of Open Access Journals (Sweden)

    C. R. Nascimento

    2014-01-01

    Full Text Available Although known as a Na,K-ATPase inhibitor, several other cellular and systemic actions have been ascribed to the steroid Ouabain (Oua. Particularly in the immune system, our group showed that Ouabain acts on decreasing lymphocyte proliferation, synergizing with glucocorticoids in spontaneous thymocyte apoptosis, and also lessening CD14 expression and blocking CD16 upregulation on human monocytes. However, Ouabain effects on dendritic cells (DCs were not explored so far. Considering the peculiar plasticity and the importance of DCs in immune responses, the aim of our study was to investigate DC maturation under Ouabain influence. To generate immature DCs, human monocytes were cultured with IL-4 and GM-CSF (5 days. To investigate Ouabain role on DC activation, DCs were stimulated with TNF-α for 48 h in the presence or absence of Ouabain. TNF-induced CD83 expression and IL-12 production were abolished in DCs incubated with 100 nM Ouabain, though DC functional capacity concerning lymphocyte activation remained unaltered. Nevertheless, TNF-α-induced antigen capture downregulation, another maturation marker, occurred even in the presence of Ouabain. Besides, Ouabain increased HLA-DR and CD86 expression, whereas CD80 expression was maintained. Collectively, our results suggest that DCs respond to Ouabain maturating into a distinct category, possibly contributing to the balance between immunity and tolerance.

  14. Effects of ouabain on ultrastructure and function in rat heart

    Institute of Scientific and Technical Information of China (English)

    JIANG Xing; GUO Ning; REN Yan-ping; Lü Zhuo-ren

    2006-01-01

    Objective:To investigate the ouabain's effects on the ultrastructure and function of the rat heart. Methods: Male Sprague-Dawley (SD) rats were treated with ouabain and systolic blood pressure (SBP) were recorded weekly. After 4 weeks, echocardiography was performed, hemodynamic parameters were measured by invasive cardiac catheterization and hanges in heart ultrastructure were analyzed using transmission electron microscopy. Results :After treated by ouabain for 4 weeks, there were no significant differences in the mean SBP of the two groups. However, cardiac systolic and diastolic performances were both worsened with ouabain treatment by echocardiography, left ventricular chamber diameters and wall thickness were significantly increased in the rats of ouabain group. Invasive monitoring indicated that left ventricular systolic pressures (LVSP), rate of pressure development (+dp/dt) and rate of pressure decay (-dp/dt) were significantly attenuated and left ventricular end-diastolic pressures (LVEDP) were increased in ouabain group (P<0. 05). Disorganization of myofilaments, mitochondrial swelling, disruption and vacuolation, hyperplastic collagen fibers were found in ouabain group by transmission electron microscopy. Conclusion:It is suggested that ouabain induces alterations in cardiac ultrastructure and function, and the effects happened before the increase of blood pressure, which indicates that ouabain might damage rat heart independent of blood pressure.

  15. Low-dose ouabain constricts small arteries from ouabain-hypertensive rats: implications for sustained elevation of vascular resistance

    OpenAIRE

    Zhang, Jin; Hamlyn, John M.; Karashima, Eiji; Raina, Hema; Mauban, Joseph R. H.; Izuka, Michelle; Berra-Romani, Roberto; Zulian, Alessandra; Wier, W. Gil; Blaustein, Mordecai P.

    2009-01-01

    Prolonged ouabain administration to normal rats causes sustained blood pressure (BP) elevation. This ouabain-induced hypertension (OH) has been attributed, in part, to the narrowing of third-order resistance arteries (∼320 μm internal diameter) as a result of collagen deposition in the artery media (see Ref. 6). Here we describe the structural and functional properties of fourth-order mesenteric small arteries from control and OH rats, including the effect of low-dose ouabain on myogenic tone...

  16. Ouabain enhances ADPKD cell apoptosis via the intrinsic pathway

    Directory of Open Access Journals (Sweden)

    Gustavo eBlanco

    2016-03-01

    Full Text Available Progression of autosomal dominant polycystic kidney disease (ADPKD is highly influenced by factors circulating in blood. We have shown that the hormone ouabain enhances several characteristics of the ADPKD cystic phenotype, including the rate of cell proliferation, fluid secretion and the capacity of the cells to form cysts. In this work, we found that physiological levels of ouabain (3nM also promote programmed cell death of renal epithelial cells obtained from kidney cysts of patients with ADPKD (ADPKD cells. This was determined by Alexa Fluor 488 labeled-Annexin-V staining and TUNEL assay, both biochemical markers of apoptosis. Ouabain-induced apoptosis also takes place when ADPKD cell growth is blocked; suggesting that the effect is not secondary to the stimulatory actions of ouabain on cell proliferation. Ouabain alters the expression of BCL family of proteins, reducing BCL-2 and increasing BAX expression levels, anti- and pro-apoptotic mediators respectively. In addition, ouabain caused the release of cytochrome c from mitochondria. Moreover, ouabain activates caspase-3, a key executioner caspase in the cell apoptotic pathway, but did not affect caspase-8. This suggests that ouabain triggers ADPKD cell apoptosis by stimulating the intrinsic, but not the extrinsic pathway of programmed cell death. The apoptotic effects of ouabain are specific for ADPKD cells and do not occur in normal human kidney cells (NHK cells. Taken together with our previous observations, these results show that ouabain causes an imbalance in cell growth/death, to favor growth of the cystic cells. This event, characteristic of ADPKD, further suggests the importance of ouabain as a circulating factor that promotes ADPKD progression.

  17. Ouabain induces cardiac remodeling in rats independent of blood pressure

    Institute of Scientific and Technical Information of China (English)

    Xing JIANG; Yan-ping REN; Zhuo-ren L(U)

    2007-01-01

    Aim: To investigate the ouabain's effects on cardiac remodeling in rats. Methods:Male Sprague-Dawley rats were treated with ouabain. Systolic blood pressure(SBP) was recorded weekly. After 4 and 6 weeks, echocardiography were performed,hemodynamic parameters were measured by invasive cardiac catheterization,changes in cardiac ultrastructure were analyzed using transmission electron microscopy, the collagen fraction of the left ventricle was assessed with Picrosirius red stain, and RT-PCR was applied to evaluate the mRNA level of myosin heavy chain-α and-β in the left ventricle. Results: Having been treated with ouabain for 4 weeks, there was no significant difference in the mean SBP of the two groups.However, left ventricular hypertrophy, myocardial ultrastructure deterioration,and extracellular matrix remodeling were induced by ouabain treatment; meanwhile,cardiac systolic and diastolic performance were both worsened. Moreover, the cardiac MHC-β mRNA was upregulated by ouabain treatment, whereas MHC-αmRNA was downregulated. After 4 weeks, the mean SBP in the ouabain group began to increase and was significantly higher than that in control group after 6 weeks (P<0.01); the rats' cardiac structure and function were worsened.Conclusion: These results suggested that ouabain induces alterations in cardiac structure and function, and the effects happened before the increase of blood pressure. The results indicated that ouabain induced cardiac remodeling in rats independent of blood pressure.

  18. Upregulation of Na+ and Ca2+ transporters in arterial smooth muscle from ouabain-induced hypertensive rats

    Science.gov (United States)

    Pulina, Maria V.; Zulian, Alessandra; Berra-Romani, Roberto; Beskina, Olga; Mazzocco-Spezzia, Amparo; Baryshnikov, Sergey G.; Papparella, Italia; Hamlyn, John M.; Blaustein, Mordecai P.

    2010-01-01

    Prolonged ouabain administration (25 μg·kg−1·day−1 for 5 wk) induces “ouabain hypertension” (OH) in rats, but the molecular mechanisms by which ouabain elevates blood pressure are unknown. Here, we compared Ca2+ signaling in mesenteric artery smooth muscle cells (ASMCs) from normotensive (NT) and OH rats. Resting cytosolic free Ca2+ concentration ([Ca2+]cyt; measured with fura-2) and phenylephrine-induced Ca2+ transients were augmented in freshly dissociated OH ASMCs. Immunoblots revealed that the expression of the ouabain-sensitive α2-subunit of Na+ pumps, but not the predominant, ouabain-resistant α1-subunit, was increased (2.5-fold vs. NT ASMCs) as was Na+/Ca2+ exchanger-1 (NCX1; 6-fold vs. NT) in OH arteries. Ca2+ entry, activated by sarcoplasmic reticulum (SR) Ca2+ store depletion with cyclopiazonic acid (SR Ca2+-ATPase inhibitor) or caffeine, was augmented in OH ASMCs. This reflected an augmented expression of 2.5-fold in OH ASMCs of C-type transient receptor potential TRPC1, an essential component of store-operated channels (SOCs); two other components of some SOCs were not expressed (TRPC4) or were not upregulated (TRPC5). Ba2+ entry activated by the diacylglycerol analog 1-oleoyl-2-acetyl-sn-glycerol [a measure of receptor-operated channel (ROC) activity] was much greater in OH than NT ASMCs. This correlated with a sixfold upregulation of TRPC6 protein, a ROC family member. Importantly, in primary cultured mesenteric ASMCs from normal rats, 72-h treatment with 100 nM ouabain significantly augmented NCX1 and TRPC6 protein expression and increased resting [Ca2+]cyt and ROC activity. SOC activity was also increased. Silencer RNA knockdown of NCX1 markedly downregulated TRPC6 and eliminated the ouabain-induced augmentation; silencer RNA knockdown of TRPC6 did not affect NCX1 expression but greatly attenuated its upregulation by ouabain. Clearly, NCX1 and TRPC6 expression are interrelated. Thus, prolonged ouabain treatment upregulates the Na+ pump

  19. On the differences between ouabain and digitalis glycosides.

    Science.gov (United States)

    Fuerstenwerth, Hauke

    2014-01-01

    Digoxin and digitoxin are widely used in the treatment of heart diseases. The exact mechanism of action of these drugs has remained an enigma. Ouabain has become the standard tool to investigate the mode of action of cardiotonic steroids, and results with ouabain are regarded as generally valid for all cardiac glycosides. However, there are marked differences between the effects of ouabain and digitalis glycosides. Ouabain has a different therapeutic profile from digitalis derivatives. Unlike digitalis glycosides, ouabain has a fast onset of action and stimulates myocardial metabolism. The inotropic effect of cardiotonic steroids is not related to inhibition of the Na-K-ATPase. Ouabain and digitalis derivatives develop their effects in different cellular spaces. Digitalis glycosides increase the intracellular calcium concentration by entering the cell interior and acting on the ryanodine receptors and by forming transmembrane calcium channels. Ouabain, by activation of the Na-K-ATPase from the extracellular side, triggers release of calcium from intracellular stores via signal transduction pathways and activates myocardial metabolism. These data no longer support the concept that all cardiotonic steroids exhibit their therapeutic effects by partial inhibition of the ion-pumping function of the Na-K-ATPase. Hence, it is suggested that this deeply rooted dogma be revised.

  20. DNA damage after intracerebroventricular injection of ouabain in rats.

    Science.gov (United States)

    Jornada, Luciano K; Valvassori, Samira S; Arent, Camila O; Leffa, Daniela; Damiani, Adriani A; Hainzenreder, Giana; Ferreira, Camila L; Moretti, Morgana; Andrade, Vanessa M; Quevedo, João

    2010-02-26

    There is an emerging body of data suggesting that bipolar disorder is associated with DNA damage. Intracerebroventricular (i.c.v.) administration of ouabain in rats results in manic-like alterations. We evaluated DNA damage of peripheral blood, cerebrospinal fluid and hippocampus of rats after i.c.v. ouabain injection. Ouabain-induced hyperlocomotion was examined in an open field. Additionally, we used single cell gel electrophoresis (comet assay) to measure early transient damage in cerebrospinal fluid (CSF), hippocampus and blood; and the micronucleus test to measure persistent damage in total blood samples of rats after ouabain administration. Our findings demonstrated that ouabain induced hyperlocomotion in rats, and this response remained up to 7 days following a single i.c.v. injection. In addition, we observed that the persistent increase in the rat spontaneous locomotion is associated with increased hippocampal and peripheral index of early DNA damage in rats. No significant alterations were observed in the micronucleus frequency in total blood samples of the rats after the ouabain i.c.v. injection. These results suggest that ouabain may induce peripheral and central early DNA damage, but this early damage may be repaired.

  1. Na,K-ATPase characterized in artificial membranes. 2. Successive measurement of ATP-driven Rb-accumulation, ouabain-blocked Rb-flux and palytoxin-induced Rb-efflux.

    Science.gov (United States)

    Anner, B M; Moosmayer, M

    1994-01-01

    The Na,K-ATPase is a multifunctional system anchored in the membrane of eukaryotic cells; it is responsible for the establishment and regulation of the Na/K balance of cell and organism by a stoichiometric mechanism linking Na extrusion to K uptake and ATP hydrolysis. The receptor for cardioactive steroids such as digoxin and ouabain is located at the extracellular surface of the system. Conversely, palytoxin, the most potent animal toxin, exerts its toxic effect by creating nonspecific leaks in the cell membrane leading to K-efflux and influx of Na and Ca ions. Ouabain prevents the pore-forming action of palytoxin in cells and therefore Na,K-ATPase is suspected to be the common receptor of ouabain and palytoxin. We have developed an artificial membrane system to determine structure-function relationships and ligand interactions of purified Na,K-ATPase: two-sided, bi-directional ATP-filled liposomes. In this system, ATP-driven 86Rb accumulation, arrest of 86Rb-uptake by ouabain, and palytoxin-induced 86Rb-leak were measured successively in the same preparation. Ouabain prevented the leak when the enzyme was ouabain-sensitive (rabbit kidney) but not when it was ouabain-resistant (rat kidney). On the basis of these data in conjunction with conformational analyses, allosteric conformational competition for the ouabain-palytoxin antagonism is proposed.

  2. Circulating inhibitor of ouabain-insensitive cation transport in malignantrenal hypertension

    Energy Technology Data Exchange (ETDEWEB)

    Simon, G.

    1986-03-01

    The role of circulating humoral agents in the pathogenesis of vascular wall Na depletion in malignant hypertension (MHT) was investigated. Plasma was collected from 33 male F344 rats with malignant one-kidney, one clip HT and 22 normotensive control rats. MHT developed spontaneously and was characterized by inactivity, weight loss, edema, anemia or hemoconcentration, hyperkalemia, and renal insufficiency. For bioassay, monolayers of quiescent vascular smooth muscle cells from F344 rats were incubated in deproteinized or whole plasma for measurement of /sup 86/Rb uptake with or without 2 mM ouabain or 1 mM furosemide. Compared to controls, ouabain-insensitive /sup 86/Rb uptake was reduced from 8.2 +- 2.0 nmol/mg protein min/sup -1/ (mean +- SD) to 5.2 +- 1.4 in deproteinized plasma (p < 0.01, N = 12) and from 6.6 +- 1.9 to 4.0 +- 0.3 in whole plasma (p < 0.05, N=5) of rats with MHT, due in part to a reduction in furosemide-sensitive uptake (p < 0.01, N = 6). There were no differences in ouabain-sensitive /sup 86/Rb uptake of cells between groups. In rats with MHT the increased Na content of the aorta that characterizes benign one-kidney, one clip HT was reversed, and bladder wall Na content was reduced (p < 0.001, N = 9). In MHT, a furosemide-like, ouabain-insensitive cation transport inhibitor in blood and urine may be the cause of vascular wall Na loss and of natriuresis that triggers the syndrome.

  3. Wistar Rats Resistant to the Hypertensive Effects of Ouabain Exhibit Enhanced Cardiac Vagal Activity and Elevated Plasma Levels of Calcitonin Gene-Related Peptide

    Science.gov (United States)

    Ghadhanfar, Elham; Al-Bader, Maie; Turcani, Marian

    2014-01-01

    Ouabain is a cardiac glycoside produced in the adrenal glands and hypothalamus. It affects the function of all cells by binding to Na+/K+-ATPase. Several lines of evidence suggest that endogenous ouabain could be involved in the pathogenesis of essential (particularly, salt-sensitive) hypertension. However, information regarding the postulated hypertensive effect of the long-term administration of low-dose exogenous ouabain is inconsistent. This study was designed to help settle this controversy through the use of telemetric monitoring of arterial blood pressure and to elucidate the ouabain-induced alterations that could either promote or prevent hypertension. Ouabain (63 and 324 µg/kg/day) was administered subcutaneously to male Wistar rats. Radiotelemetry was used to monitor blood pressure, heart rate and measures of cardiovascular variability and baroreflex sensitivity. The continuous administration of ouabain for 3 months did not elevate arterial blood pressure. The low-frequency power of systolic pressure variability, urinary excretion of catecholamines, and cardiovascular response to restraint stress and a high-salt diet as well as the responsiveness to α1-adrenergic stimulation were all unaltered by ouabain administration, suggesting that the activity of the sympathetic nervous system was not increased. However, surrogate indices of cardiac vagal nerve activity based on heart rate variability were elevated. Molecular remodeling in mesenteric arteries that could support the development of hypertension (increased expression of the genes for the Na+/Ca2+ exchanger and Na+/K+-ATPase α2 isoform) was not evident. Instead, the plasma level of vasodilatory calcitonin gene-related peptide (CGRP) significantly rose from 55 (11, SD) in the control group to 89 (20, SD) pg/ml in the ouabain-treated rats (PTukey's = 18.10−5). These data show that long-term administration of exogenous ouabain does not necessarily cause hypertension in rodents. The augmented

  4. Innovative nanostructures for highly sensitive vibrational biosensing (Conference Presentation)

    Science.gov (United States)

    Popp, Juergen; Mayerhöfer, Thomas; Cialla-May, Dana; Weber, Karina; Huebner, Uwe

    2016-03-01

    Employing vibrational spectroscopy (IR-absorption and Raman spectroscopy) allows for the labelfree detection of molecular specific fingerprints of inorganic, organic and biological substances. The sensitivity of vibrational spectroscopy can be improved by several orders of magnitude via the application of plasmonic active surfaces. Within this contribution we will discuss two such approaches, namely surface enhanced Raman spectroscopy (SERS) as well as surface enhanced IR absorption (SEIRA). It will be shown that SERS using metal colloids as SERS active substrate in combination with a microfluidic lab-on-a-chip (LOC) device enables high throughput and reproducible measurements with highest sensitivity and specificity. The application of such a LOC-SERS approach for therapeutic drug monitoring (e.g. quantitative detection of antibiotics in a urine matrix) will be presented. Furthermore, we will introduce innovative bottom-up strategies to prepare SERS-active nanostructures coated with a lipophilic sensor layer as one-time use SERS substrates for specific food analysis (e.g. quantitative detection of toxic food colorants). The second part of this contribution presents a slit array metamaterial perfect absorber for IR sensing applications consisting of a dielectric layer sandwiched between two metallic layers of which the upper layer is perforated with a periodic array of slits. Light-matter interaction is greatly amplified in the slits, where also the analyte is concentrated, as the surface of the substrate is covered by a thin silica layer. Thus, already small concentrations of analytes down to a monolayer can be detected by refractive index sensing and identified by their spectral fingerprints with a standard mid-infrared lab spectrometer.

  5. Strain-sensitive upconversion for imaging biological forces (Conference Presentation)

    Science.gov (United States)

    Lay, Alice; Wisser, Michael; Lin, Yu; Narayan, Tarun; Krieg, Michael; Atre, Ashwin; Goodman, Miriam; Dionne, Jennifer A.

    2016-09-01

    Nearly all diseases can be traced back to abnormal mechanotransduction, but few sensors can reliably measure biologically-relevant forces in vivo. Here, we investigate sub-25nm lanthanide-doped upconverting nanoparticles as novel optical force probes, which provide several biocompatible features: sharp emission peaks with near infrared illumination, a high signal-to-noise ratio, and photostability. To increase force sensitivity, we include d-metal doping in the nanoparticles; the d-metal siphons energy from the lanthanide ions with an efficiency that varies with pressure. We synthesize cubic-phase NaYF4: Er3+,Yb3+ nanoparticles doped with 0-5% Mn2+ and compress them in a hydrostatic environment using a diamond anvil cell. When illuminated at 980nm, the nanoparticles show sharp emission peaks centered at wavelengths of 522nm, 545nm, and 660nm. In 20nN increments, up to 700nN, the ratio of the red-to-green peaks in 0% Mn-doped nanoparticles increases by nearly 30%, resulting in a perceived color change from orange to red. In contrast, the 1% Mn-doped samples exhibit little color change but a large 40% decrease in upconversion intensity. In both cases, the red-to-green ratio varies linearly with strain and the optical properties are recoverable upon release. We further use atomic force microscopy to characterize optical responses at lower, pico-Newton to nano-Newton forces. To demonstrate in vivo imaging capabilities, we incubate C. elegans with nanoparticles dispersed in buffer solution (5mg/mL concentration) and image forces involved in digestion using confocal microscopy. Our nanoparticles provide a platform for the first, non-genetically-encoded in vivo force sensors, and we describe routes to increase their sensitivity to the single-pN range.

  6. Phase-sensitive multiple reference optical coherence tomography (Conference Presentation)

    Science.gov (United States)

    Dsouza, Roshan I.; Subhash, Hrebesh; Neuhaus, Kai; Hogan, Josh; Wilson, Carol; Leahy, Martin

    2016-03-01

    Multiple reference OCT (MR-OCT) is a recently developed novel time-domain OCT platform based on a miniature reference arm optical delay, which utilizes a single miniature actuator and a partial mirror to generate recirculating optical delay for extended axial-scan range. MR-OCT technology promises to fit into a robust and cost-effective design, compatible with integration into consumer-level devices for addressing wide applications in mobile healthcare and biometry applications. Using conventional intensity based OCT processing techniques, the high-resolution structural imaging capability of MR-OCT has been recently demonstrated for various applications including in vivo human samples. In this study, we demonstrate the feasibility of implementing phase based processing with MR-OCT for various functional applications such as Doppler imaging and sensing of blood vessels, and for tissue vibrography applications. The MR-OCT system operates at 1310nm with a spatial resolution of ~26 µm and an axial scan rate of 600Hz. Initial studies show a displacement-sensitivity of ~20 nm to ~120 nm for the first 1 to 9 orders of reflections, respectively with a mirror as test-sample. The corresponding minimum resolvable velocity for these orders are ~2.3 µm/sec and ~15 µm/sec respectively. Data from a chick chorioallantoic membrane (CAM) model will be shown to demonstrate the feasibility of MR-OCT for imaging in-vivo blood flow.

  7. Ouabain, a steroid hormone that signals with slow calcium oscillations

    OpenAIRE

    2001-01-01

    The plant-derived steroid, digoxin, a specific inhibitor of Na,K-ATPase, has been used for centuries in the treatment of heart disease. Recent studies demonstrate the presence of a digoxin analog, ouabain, in mammalian tissue, but its biological role has not been elucidated. Here, we show in renal epithelial cells that ouabain, in doses causing only partial Na,K-ATPase inhibition, acts as a biological inducer of regular, low-frequency intracellular calcium ([Ca2+]i) oscillations that elicit a...

  8. Reversal by EGTA of the enhanced secretory responsiveness of mast cells due to treatment with ouabain

    DEFF Research Database (Denmark)

    Johansen, Torben; Knudsen, T; Bertelsen, Niels Haldor

    1990-01-01

    The effect of EGTA on the enhancement by ouabain of compound 48/80-induced secretion from mast cells was compared with the effect on the Na(+)-K+ pump activity. The time-dependent secretory enhancement by ouabain was blocked by addition of EGTA to the cell suspension concomitantly with the addition...... of ouabain, and EGTA caused a large increase in the pump activity. Addition of 10 microM EGTA to ouabain-treated cells stopped but did not reverse the enhancement. The experiments show that the effect of ouabain was due to changes in a calcium pool utilized in compound 48/80-induced secretion following...

  9. Ouabain, a steroid hormone that signals with slow calcium oscillations.

    Science.gov (United States)

    Aizman, O; Uhlén, P; Lal, M; Brismar, H; Aperia, A

    2001-11-06

    The plant-derived steroid, digoxin, a specific inhibitor of Na,K-ATPase, has been used for centuries in the treatment of heart disease. Recent studies demonstrate the presence of a digoxin analog, ouabain, in mammalian tissue, but its biological role has not been elucidated. Here, we show in renal epithelial cells that ouabain, in doses causing only partial Na,K-ATPase inhibition, acts as a biological inducer of regular, low-frequency intracellular calcium ([Ca(2+)](i)) oscillations that elicit activation of the transcription factor, NF-kappa B. Partial inhibition of Na,K-ATPase using low extracellular K(+) and depolarization of cells did not have these effects. Incubation of cells in Ca(2+)-free media, inhibition of voltage-gated calcium channels, inositol triphosphate receptor antagonism, and redistribution of actin to a thick layer adjacent to the plasma membrane abolished [Ca(2+)](i) oscillations, indicating that they were caused by a concerted action of inositol triphosphate receptors and capacitative calcium entry via plasma membrane channels. Blockade of ouabain-induced [Ca(2+)](i) oscillations prevented activation of NF-kappa B. The results demonstrate a new mechanism for steroid signaling via plasma membrane receptors and underline a novel role for the steroid hormone, ouabain, as a physiological inducer of [Ca(2+)](i) oscillations involved in transcriptional regulation in mammalian cells.

  10. Evaluating a Skin Sensitization Model and Examining Common Assumptions of Skin Sensitizers (QSAR conference)

    Science.gov (United States)

    Skin sensitization is an adverse outcome that has been well studied over many decades. Knowledge of the mechanism of action was recently summarized using the Adverse Outcome Pathway (AOP) framework as part of the OECD work programme (OECD, 2012). Currently there is a strong focus...

  11. Nanomolar ouabain elicits apoptosis through a direct action on HeLa cell mitochondria.

    Science.gov (United States)

    Alonso, Elba; Cano-Abad, María F; Moreno-Ortega, Ana J; Novalbos, Jesús; Milla, Juan; García, Antonio G; Ruiz-Nuño, Ana

    2013-11-01

    The steroid Na(+)/K(+) ATPase (NKA) blocker ouabain has been shown to exhibit pro-apoptotic effects in various cell systems; however, the mechanism involved in those effects is unclear. Here, we have demonstrated that incubation of HeLa cells during 24h with nanomolar concentrations of ouabain or digoxin causes apoptotic death of 30-50% of the cells. Ouabain caused the activation of caspases-3/7 and -9; however, caspase-8 was unaffected. The fact that compound Z-LEHD-FMK reduced both apoptosis and caspase-9 activation elicited by ouabain, suggest a mitochondrially-mediated pathway. This was strengthened by the fact that ouabain caused ATP depletion and the release of mitochondrial cytochrome c into the cytosol. Furthermore, upon ouabain treatment mitochondrial disruption and redistribution into the cytosol were observed. A mitochondrial site of action for ouabain was further corroborated by tight co-localisation of fluorescent ouabain with mitochondria. Finally, in ouabain-treated cells the histamine-elicited elevation of cytosolic Ca(2+) concentration ([Ca(2+)]c) suggests an additional effect on the endoplasmic reticulum (ER) leading to Ca(2+) store depletion. We conclude that fluorescent ouabain is taken up and tightly co-localises with mitochondria of HeLa cells. This indicates that apoptosis may be triggered by a direct action of ouabain on mitochondria.

  12. Ouabain exacerbates botulinum neurotoxin-induced muscle paralysis via progression of muscle atrophy in mice.

    Science.gov (United States)

    Fujikawa, Ryu; Muroi, Yoshikage; Unno, Toshihiro; Ishii, Toshiaki

    2010-12-01

    Botulinum neurotoxin serotype A (BoNT/A) inhibits acetylcholine release at the neuromuscular junction in isolated muscles, and ouabain can partially block its effect. However, it is not clear whether ouabain attenuates BoNT/A-induced neuromuscular paralysis in vivo. In this work, we investigated the effects of ouabain on BoNT/A-induced neuromuscular paralysis in mice. Ouabain was administered to mice intraperitoneally immediately after a single injection of BoNT/A into skeletal muscle. The effects of ouabain on BoNT/A-induced muscle paralysis were assessed by quantitative monitoring of muscle tension and digit abduction via the digit abduction scoring (DAS) assay. A single administration of ouabain significantly prolonged BoNT/A-induced neuromuscular paralysis. Moreover, consecutive daily injection of ouabain exacerbated BoNT/A-induced neuromuscular paralysis, and led to a significant decrease in both twitch and tetanic forces as assayed in isolated BoNT/A-injected muscles. We next looked at the effects of ouabain on BoNT/A-induced muscle atrophy. Administration of ouabain led to a decrease in the myofibrillar cross-sectional area (CSAs) by 14 post-BoNT/A injection. In addition, repeated administration of ouabain increased mRNA expression levels of ubiquitin ligases, which are markers of muscle atrophy, in BoNT/A-injected muscle. These results suggest that ouabain exacerbates BoNT/A-induced neuromuscular paralysis via a marked progression of BoNT/A-induced muscle atrophy.

  13. Low-dose ouabain constricts small arteries from ouabain-hypertensive rats: implications for sustained elevation of vascular resistance

    Science.gov (United States)

    Zhang, Jin; Hamlyn, John M.; Karashima, Eiji; Raina, Hema; Mauban, Joseph R. H.; Izuka, Michelle; Berra-Romani, Roberto; Zulian, Alessandra; Wier, W. Gil; Blaustein, Mordecai P.

    2009-01-01

    Prolonged ouabain administration to normal rats causes sustained blood pressure (BP) elevation. This ouabain-induced hypertension (OH) has been attributed, in part, to the narrowing of third-order resistance arteries (∼320 μm internal diameter) as a result of collagen deposition in the artery media (see Ref. 6). Here we describe the structural and functional properties of fourth-order mesenteric small arteries from control and OH rats, including the effect of low-dose ouabain on myogenic tone in these arteries. Systolic BP in OH rats was 138 ± 3 versus 124 ± 4 mmHg in controls (P < 0.01). Pressurized (70 mmHg) control and OH arteries, with only a single layer of myocytes, both had ∼165-μm internal diameters and ∼20-μm wall thicknesses. Even after fixation, despite vasoconstriction, the diameters and wall thicknesses did not differ between control and OH fourth-order arteries, whereas in third-order arteries, both parameters were significantly smaller in OH than in controls. Myogenic reactivity was significantly augmented in OH fourth-order arteries. Nevertheless, phenylephrine- (1 μM) and high K+-induced vasoconstrictions and acetylcholine-induced vasodilation were comparable in control and OH arteries. Vasoconstrictions induced by 5 μM phenylephrine and by 10 mM caffeine in Ca2+-free media indicated that releasable sarcoplasmic reticulum Ca2+ stores were normal in OH arteries. Importantly, 100 nM ouabain constricted both control and OH arteries by ∼26 μm, indicating that this response was not downregulated in OH rats. This maximal ouabain-induced constriction corresponds to a ∼90% increase in resistance to flow in these small arteries; thus ouabain at EC50 of ∼0.66 nM should raise resistance by ∼35%. We conclude that dynamic constriction in response to circulating nanomolar ouabain in small arteries likely makes a major contribution to the increased vascular tone and BP in OH rats. PMID:19617413

  14. Stabilisation of Na,K-ATPase structure by the cardiotonic steroid ouabain

    Energy Technology Data Exchange (ETDEWEB)

    Miles, Andrew J. [Institute of Structural and Molecular Biology, Birkbeck College, University of London, London WC1E 7HX (United Kingdom); Fedosova, Natalya U. [Department of Biomedicine, Aarhus University, DK-8000 Aarhus (Denmark); Hoffmann, Søren V. [ISA, Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus (Denmark); Wallace, B.A. [Institute of Structural and Molecular Biology, Birkbeck College, University of London, London WC1E 7HX (United Kingdom); Esmann, Mikael, E-mail: me@biophys.au.dk [Department of Biomedicine, Aarhus University, DK-8000 Aarhus (Denmark)

    2013-05-31

    Highlights: •Ouabain binding to pig and shark Na,K-ATPase enhances thermal stability. •Ouabain stabilises both membrane-bound and solubilised Na,K-ATPase. •Synchrotron radiation circular dichroism is used for structure determination. •Secondary structure in general is not affected by ouabain binding. •Stabilisation is due to re-arrangement of tertiary structure. -- Abstract: Cardiotonic steroids such as ouabain bind with high affinity to the membrane-bound cation-transporting P-type Na,K-ATPase, leading to complete inhibition of the enzyme. Using synchrotron radiation circular dichroism spectroscopy we show that the enzyme-ouabain complex is less susceptible to thermal denaturation (unfolding) than the ouabain-free enzyme, and this protection is observed with Na,K-ATPase purified from pig kidney as well as from shark rectal glands. It is also shown that detergent-solubilised preparations of Na,K-ATPase are stabilised by ouabain, which could account for the successful crystallisation of Na,K-ATPase in the ouabain-bound form. The secondary structure is not significantly affected by the binding of ouabain. Ouabain appears however, to induce a reorganization of the tertiary structure towards a more compact protein structure which is less prone to unfolding; recent crystal structures of the two enzymes are consistent with this interpretation. These circular dichroism spectroscopic studies in solution therefore provide complementary information to that provided by crystallography.

  15. Effect of glucose intake on human leucocyte /sup 86/Rb influx and (/sup 3/H)-ouabain binding

    Energy Technology Data Exchange (ETDEWEB)

    Turaihi, K.; Baron, D.N.; Dandona, P.

    1988-02-01

    /sup 86/Rb influx and (/sup 3/H) ouabain binding by human leucocytes were measured in eight normal nonobese fasting subjects before and after a challenge with 75 g glucose orally. The mean ouabain-sensitive /sup 86/Rb influx increased significantly from 194 to 283 mmol/kg protein/h (P less than .01), and (/sup 3/H)-ouabain binding increased from 236 to 403 fmol/mg protein. The mean plasma potassium concentration fell from 4.2 to 3.9 mmol/L (P less than .05). Following intravenous glucose infusion, the median /sup 86/Rb transport increased from 186 to 267 mmol/kg protein/h, while median plasma potassium concentration fell from 4.3 to 3.9 mmol/L. Therefore, glucose intake acutely increases Na-K ATPase units, stimulates potassium (Rb) transport, and causes a concomitant fall in plasma potassium concentrations. Nutritional intake is probably an important determinant of Na-K ATPase units and activity in the human leucocyte.

  16. Natriuretic Hormones, Endogenous Ouabain, and Related Sodium Transport Inhibitors

    Directory of Open Access Journals (Sweden)

    John eHamlyn

    2014-12-01

    Full Text Available The work of deWardener and colleagues stimulated longstanding interest in natriuretic hormones (NH. In addition to the atrial peptides (APs, the circulation contains unidentified physiologically-relevant NHs. One NH is controlled by the central nervous system (CNS and likely secreted by the pituitary. Its circulating activity is modulated by salt intake and the prevailing sodium concentration of the blood and intracerebroventricular fluid, and contributes to postprandial and dehydration natriuresis. The other NH, mobilized by atrial stretch, promotes natriuresis by increasing the production of intrarenal dopamine and/or nitric oxide. Both NHs have short (<35 minutes circulating half lives, depress renotubular sodium transport, and neither requires the renal nerves. The search for NHs led to endogenous cardiotonic steroids (CTS including ouabain-, digoxin-, and bufadienolide-like materials. These CTS, given acutely in high nanomole to micromole amounts into the general or renal circulations, inhibit sodium pumps and are natriuretic. Among these CTS, only bufalin is cleared sufficiently rapidly to qualify for an NH-like role. Ouabain-like CTS are cleared slowly, and when given chronically in low daily nanomole amounts, promote sodium retention, augment arterial myogenic tone, reduce renal blood flow and glomerular filtration, suppress nitric oxide in the renal vasa recta, and increase sympathetic nerve activity and blood pressure. Moreover, lowering total body sodium raises circulating endogenous ouabain. Thus, ouabain-like CTS have physiological actions that, like aldosterone, support renal sodium retention and blood pressure. In conclusion, the mammalian circulation contains two non-AP NHs. Identification of the CNS NH should be a priority.

  17. EXPRESSION OF ENDOGENOUS OUABAIN IN MULTIPLE ADRENAL TUMORS

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective To explore expression of endogenous ouabain(EO) in multiple adrenal tumors.Methods Thirty-one cases of adrenal tumors and 6 cases of healthy adrenal tissues were selected. The expression of EO in the adrenal tiss ue was detected with immunohistochemical streptavidin peroxadase conjugated(SP) method.Results Most of EO positive products were localized in cy toplasm of the zona reticularis of human adrenal cortex, and positive products s howed to be fine granular. There was no positive signal in the medulla. EO showe d on diffused positive in patients with pheochromocytoma accompanied high blood pressure[SBP:(165.22±7.61) mmHg, DBP:(105.52±4.26) mmHg], but there were neg ative in ones with normative blood pressure[SBP:(118.52±4.58) mmHg, DBP:(83±3.60) m mHg]. The expression of EO was positive in all adrenocortical hyperplasic, aden oma an d carcinoma, no matter its high or normative blood pressure. The degree of expre ssion of EO in adrenal tissues was related to the level of BP.Conclusion Expression of endogenous ouabain(EO) in health y adrenal tissue and adrenal tumors was a valuable morphological and pathophysio logical clue for the research on ouabain.

  18. Salt, endogenous ouabain and blood pressure interactions in the general population

    OpenAIRE

    Manunta, P.; Wang,JG; G. Bianchi; Messaggio, E.; Fagard, Robert; Nawrot, Tim; Staessen, Jan A.; Hamlyn, JM

    2003-01-01

    OBJECTIVE: Experimental data show that ouabain is a modulator of the sodium-potassium pump, which plays an important role in sodium homeostasis and blood pressure regulation. We investigated the distribution of plasma ouabain in the general population in relation to blood pressure and other determinants of sodium homeostasis. METHODS: In 379 subjects enrolled in a Belgian population study, we measured plasma ouabain, clinical characteristics including blood pressure, serum and urinary electro...

  19. Crizotinib-resistant NPM-ALK mutants confer differential sensitivity to unrelated Alk inhibitors.

    Science.gov (United States)

    Ceccon, Monica; Mologni, Luca; Bisson, William; Scapozza, Leonardo; Gambacorti-Passerini, Carlo

    2013-02-01

    The dual ALK/MET inhibitor crizotinib was recently approved for the treatment of metastatic and late-stage ALK+ NSCLC, and is currently in clinical trial for other ALK-related diseases. As predicted after other tyrosine kinase inhibitors' clinical experience, the first mutations that confer resistance to crizotinib have been described in patients with non-small cell lung cancer (NSCLC) and in one patient inflammatory myofibroblastic tumor (IMT). Here, we focused our attention on the anaplastic large cell lymphoma (ALCL), where the oncogenic fusion protein NPM-ALK, responsible for 70% to 80% of cases, represents an ideal crizotinib target. We selected and characterized 2 human NPM-ALK+ ALCL cell lines, KARPAS-299 and SUP-M2, able to survive and proliferate at different crizotinib concentrations. Sequencing of ALK kinase domain revealed that a single mutation became predominant at high crizotinib doses in each cell line, namely L1196Q and I1171N in Karpas-299 and SUP-M2 cells, respectively. These mutations also conferred resistance to crizotinib in Ba/F3 cells expressing human NPM-ALK. The resistant cell populations, as well as mutated Ba/F3 cells, were characterized for sensitivity to two additional ALK inhibitors: the dual ALK/EGFR inhibitor AP26113 and NVP-TAE684. While L1196Q-positive cell lines were sensitive to both inhibitors, cells carrying I1171N substitution showed cross-resistance to all ALK inhibitors tested. This study provides potentially relevant information for the management of patients with ALCL that may relapse after crizotinib treatment.

  20. S-glutathionylation of an auxiliary subunit confers redox sensitivity to Kv4 channel inactivation.

    Directory of Open Access Journals (Sweden)

    Henry H Jerng

    Full Text Available Reactive oxygen species (ROS regulate ion channels, modulate neuronal excitability, and contribute to the etiology of neurodegenerative disorders. ROS differentially suppress fast "ball-and-chain" N-type inactivation of cloned Kv1 and Kv3 potassium channels but not of Kv4 channels, likely due to a lack of reactive cysteines in Kv4 N-termini. Recently, we discovered that N-type inactivation of Kv4 channel complexes can be independently conferred by certain N-terminal variants of Kv4 auxiliary subunits (DPP6a, DPP10a. Here, we report that both DPP6a and DPP10a, like Kv subunits with redox-sensitive N-type inactivation, contain a highly conserved cysteine in their N-termini (Cys-13. To test if N-type inactivation mediated by DPP6a or DPP10a is redox sensitive, Xenopus oocyte recordings were performed to examine the effects of two common oxidants, tert-butyl hydroperoxide (tBHP and diamide. Both oxidants markedly modulate DPP6a- or DPP10a-conferred N-type inactivation of Kv4 channels, slowing the overall inactivation and increasing the peak current. These functional effects are fully reversed by the reducing agent dithiothreitol (DTT and appear to be due to a selective modulation of the N-type inactivation mediated by these auxiliary subunits. Mutation of DPP6a Cys-13 to serine eliminated the tBHP or diamide effects, confirming the importance of Cys-13 to the oxidative regulation. Biochemical studies designed to elucidate the underlying molecular mechanism show no evidence of protein-protein disulfide linkage formation following cysteine oxidation. Instead, using a biotinylated glutathione (BioGEE reagent, we discovered that oxidation by tBHP or diamide leads to S-glutathionylation of Cys-13, suggesting that S-glutathionylation underlies the regulation of fast N-type inactivation by redox. In conclusion, our studies suggest that Kv4-based A-type current in neurons may show differential redox sensitivity depending on whether DPP6a or DPP10a is highly

  1. THE CHARACTERISTICS OF ENDOGENOUS OUABAIN SECRETIONFROM CULTURED BOVINE ADRENOCORTICAL CELLS

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective To compare the characteristics of endogenous ouabain(EO) secretion with the other adrenocortical hormones and determine the effects of angiotensin Ⅰ (Ang Ⅰ ), and adrenocorticotrophin(ACTH) on the secretion of EO. Methods EO was measured by radioimmunoassay from primary cultured bovine adrenocotical cells (BAC). Results ①Ouabain was determined in the media of cultured BAC. Both EO and aldosterone secretion were decreased from the outer to inner layer of the cultured adrenal cortex, and the responses to Ang Ⅰ and ACTH were higher than that in the mid layer (P <0. 05) and inner layer (P <0. 01). Cortisol secretion was activated by Ang Ⅱ or ACTH was significantly higher in the mid layer and in the inner layer than that in the outer layer. ②The time-course experiment showed that the gradually rising amounts of aldosterone and cortisol could be determined dur ing the continuous incubation to 48h with or without Ang Ⅰ or ACTH. However, EO did not increase continuously af ter 24h of incubation in the basal secreting situation and after 12h of incubation in the stimulating situation by Ang Ⅱ or ACTH. ③There were obvious drops in aldosterone and cortisol secretion from 3rd day during a 21 day-period cell culture, but the peak secretion of ouabain was in 7th day. Conclusion It suggests that the secretory mechanism might be different between EO and aldosterone or cortisol. Also, Ang Ⅱ and ACTH might be involved in the regulation of EO secretion.

  2. Detection of ouabain-insensitive H(+)-transporting, K(+)-stimulated p-nitrophenylphosphatase activity in rat gastric glands by cerium-based cytochemistry.

    Science.gov (United States)

    Kobayashi, T; Seguchi, H

    1990-12-01

    We employed a modification of our previously reported cerium-based cytochemical method for ouabain-sensitive, K-dependent p-nitrophenylphosphatase (Na-K ATPase) activity to detect ouabain-insensitive, K-stimulated p-nitrophenylphosphatase (K-pNPPase) activity in rat gastric glands. Biochemically, the enzyme activity of gastric glands incubated in a medium containing 50 mM Tricine buffer (pH 7.5), 50 mM KCl, 10 mM MgCl2, 2 mM CeCl3, 2 mM p-nitrophenylphosphate (pNPP), 2.5 mM levamisole, 10 mM ouabain, and 0.00015% Triton X-100, was optimal at pH 7.5-8.0 and decreased above pH 8.5. The amount of p-nitrophenol after incubation increased linearly in proportion to the amount of tissue in the medium. The enzyme activity was inhibited by omeprazole, sodium flouride (NaF), N-ethylmaleimide (NEM), and dicyclohexylcarbodiimide (DCCD). Heat-treated specimens had no enzyme activity. The enzyme activity increased with addition of K ions up to the concentration of 50 mM, and became constant above 50 mM. Cytochemically, the parietal cells of the gastric glands reacted positively for ouabain-insensitive K-pNPPase activity. Intense reaction was observed at the microvilli of the luminal surface and the intracellular canaliculi. The tubulovesicular system showed weak enzyme activity. The reaction products were found as fine, granular, electron-dense deposits in the cytoplasm just beneath the plasma membrane. The ouabain-insensitive K-pNPPase activity detected in this study appears, therefore, to be associated with that of H-transporting, K-stimulated adenosine triphosphatase (H-K ATPase).

  3. Training increases the concentration of [3H]ouabain-binding sites in rat skeletal muscle

    DEFF Research Database (Denmark)

    Kjeldsen, K; Richter, Erik; Galbo, H

    1986-01-01

    ]ouabain-binding-site concentration in the diaphragm, but in the heart ventricles, the K+-dependent 3-O-methylfluorescein phosphatase activity increased by 20% (P less than 0.001). Muscle inactivity induced by denervation, plaster immobilisation or tenotomy reduced the [3H]ouabain-binding-site concentration by 20-30% (P less than 0...

  4. Amino acid substitutions of Na,K-ATPase conferring decreased sensitivity to cardenolides in insects compared to mammals

    NARCIS (Netherlands)

    Dalla, S.; Swarts, H.G.P.; Koenderink, J.B.; Dobler, S.

    2013-01-01

    Mutagenesis analyses and a recent crystal structure of the mammalian Na,K-ATPase have identified amino acids which are responsible for high affinity binding of cardenolides (such as ouabain) which at higher doses block the enzyme in the phosphorylated state. Genetic analysis of the Na,K-ATPase of in

  5. On the release of catecholamines and dopamine-beta-hydroxylase evoked by ouabain in the perfused cat adrenal gland.

    OpenAIRE

    Garcia, A. G.; Hernandez, M.; Horga, J. F.; Sanchez-Garcia, P.

    1980-01-01

    1 Secretion of catecholamines (CA) and dopamine-beta-hydroxylase (DBH) activity from the retrogradely perfused cat adrenal gland was studied following ouabain infusion. Perfusion with ouabain (10(-4) M) for 10 min caused a gradual release of CA in the effluent which reached its peak 30 min after the ouabain pulse, and was maintained constant for at least 1 h. The effect of ouabain seemed to be irreversible. 2 Mecamylamine, while blocking the CA secretory effects of acetylcholine (ACh) perfusi...

  6. Ouabain facilitates cardiac differentiation of mouse embryonic stem cells through ERK1/2 pathway

    Institute of Scientific and Technical Information of China (English)

    Yee-ki LEE; Kwong-man NG; Wing-hon LAI; Cornelia MAN; Deborah K LIEU; Chu-pak LAU; Hung-fat TSE; Chung-wah SIU

    2011-01-01

    Aim:To investigate the effects of the cardiotonic steroid, ouabain, on cardiac differentiation of murine embyronic stem cells (mESCs).Methods:Cardiac differentiation of murine ESCs was enhanced by standard hanging drop method in the presence of ouabain (20 μmol/L) for 7 d. The dissociated ES derived cardiomyocytes were examined by flow cytometry, RT-PCR and confocal calcium imaging.Results:Compared with control, mESCs treated with ouabain (20 μmol/L) yielded a significantly higher percentage of cardiomyocytesand significantly increased expression of a panel of cardiac markers including Nkx 2.5, α-MHC, and β-MHC. The α1 and 2- isoforms Na+/K+ -ATPase, on which ouabain acted, were also increased in mESCs during differentiation. Among the three MAPKs involved in the cardiac hypertrophy pathway, ouabain enhanced ERK1/2 activation. Blockage of the Erk1/2 pathway by U0126 (10 μmol/L) inhibited cardiac differentiation while ouabain (20 μmol/L) rescued the effect. Interestingly, the expression of calcium handling proteins, includ ing ryanodine receptor (RyR2) and sacroplasmic recticulum Ca2+ ATPase (SERCA2a) was also upregulated in ouabain-treated mESCs.ESC-derived cardiomyocyes (CM) treated with ouabain appeared to have more mature calcium handling. As demonstrated by confocal Ca2+ imaging, cardiomyocytes isolated from ouabain-treated mESCs exhibited higher maximum upstroke velocity (P<0.01) and maximum decay velocity (P<0.05), as well as a higher amplitude of caffeine induced Ca2+ transient (P<0.05), suggesting more mature sarcoplasmic reticulum (SR).Conclusion:Ouabain induces cardiac differentiation and maturation of mESC-derived cardiomyocytes via activation of Erk1/2 and more mature SR for calcium handling.

  7. Effect of ouabain on the pathogenesis of hypertension in rats

    Institute of Scientific and Technical Information of China (English)

    Liu Meiyan; Ren Yanping; Guo Chengjun

    2014-01-01

    Background Ouabain and digoxin are important cardiac glycoside and related to many cardiovascular diseases.The purpose of this study was to investigate the changes of sodium pump α-subunit expression in rats and compare the effects of ouabain (OUA) and digoxin (DIG) on the development of hypertension.Methods In situ hybridization was performed.Specific sequence oligonucleotide probe tailing with a Dig-dUTP hybrid to target nucleic acids of the sodium pump α-subunit.According to counting positive particles sodium pump subunit expression was analyzed with statistical methods.Results On day 16 of drug administration,the blood pressure of rats increased significantly in the OUA group.In the DIG group,the blood pressure revealed no significant difference when compared to the control group.In addition,the effects of OUA and DIG on sodium pump α-subunit RNA expression in tissues differed.Conclusions OUA and DIG can not only change the configuration of the sodium pump to depress their activity,but also influence their gene expression which is important in the mechanism of hypertension.This may be a key point in the pathogenesis of hypertension in the manner in which OUA differs from DIG and changes the sodium pump gene expression in the arteries and kidneys of rats.

  8. Endogenous ouabain in renal Na(+) handling and related diseases.

    Science.gov (United States)

    Manunta, Paolo; Messaggio, Elisabetta; Casamassima, Nunzia; Gatti, Guido; Carpini, Simona Delli; Zagato, Laura; Hamlyn, John M

    2010-12-01

    The Na(+) pump and its Endogenous modulator Ouabain (EO) can be considered as an ancestral enzymatic system, conserved among species ranging from Drosophila to humans, related to Na handling. In this review, we examine how EO is linked with vascular function in hypertension and if it impacts the pathogenesis of heart and renal failure. Moreover, the molecular mechanism of endogenous ouabain-linked hypertension involves the sodium pump/sodium-calcium exchanger duet. Biosynthesis of EO occurs in adrenal glands and is under the control of angiotensin II, ACTH and epinephrine. Elevated concentrations of EO and in the sub-nanomolar concentration range were found to stimulate proliferation and differentiation of cardiac and smooth muscle cells. They may have a primary role in the development of cardiac dysfunction and failure. Experimental data suggest that the Na/K-ATPase α(2)-catalytic subunit causes EO-induced vasoconstriction. Finally, maneuvers that promote Na depletion, as diuretic therapy or reduced Na intake, raise the EO levels. Taken together, these findings suggest a key role for EO in body Na homeostasis.

  9. Intracerebroventricular administration of ouabain alters synaptic plasticity and dopamine release in rat medial prefrontal cortex.

    Science.gov (United States)

    Sui, Li; Song, Xiao-Jin; Ren, Jie; Ju, Li-Hua; Wang, Yan

    2013-08-01

    Intracerebroventricular (ICV) administration of ouabain, a specific Na-K-ATPase inhibitor, in rats mimics the manic phenotypes of bipolar disorder and thus has been proposed as one of the best animal models of mania. Bipolar mania has been known to be associated with dysfunctions of medial prefrontal cortex (mPFC), a brain area critically involved in mental functions; however, the exact mechanism underlying these dysfunctions is not yet clear. The present study investigated synaptic transmission, synaptic plasticity, and dopamine release in Sprague-Dawley rat mPFC following ICV administration of ouabain (5 μl of 1 mM ouabain). The electrophysiological results demonstrated that ouabain depressed the short- and the long-term synaptic plasticity, represented by paired-pulse facilitation and long-term potentiation, respectively, in the mPFC. These ouabain-induced alterations in synaptic plasticity can be prevented by pre-treatment with lithium (intraperitoneal injection of 47.5 mg/kg lithium, twice a day, 7 days), which acts as an effective mood stabilizer in preventing mania. The electrochemical results demonstrated that ICV administration of ouabain enhanced dopamine release in the mPFC, which did not be affected by pre-treatment with lithium. These findings suggested that alterations in synaptic plasticity and dopamine release in the mPFC might underlie the dysfunctions of mPFC accompanied with ouabain administration-induced bipolar mania.

  10. EXPERIMENTAL STUDY ON THE ROLES OF ENDOGENOUS OUABAIN IN THE DEVELOPMENT OF HYPERTENSION

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective To explore the relationship between endogenous ouabain and the pathogenesis of hyper- tension. Methods ① Sixteen Sprague-Dawley (SD) rats were selected and randomly divided into two groups, and then the rats were injected with ouabain in dosage of 20μg/kg. d and normal saline (NS), respectively. The indirect systolic blood pressure of all the rats were recorded once a week. ②) Twenty-five lk1c (one kidney and one clipped) hypertensive rats were established and injected randomly with anti-ouabain antibody, normal rabbit IgG and normal saline, respectively. The direct blood pressure of all the lklc hypertensive rats were recorded by cannulated carotid artery for 3h after administration. Results The systolic blood pressure of rats injected with ouabain began to increase 2 weeks after ouabain administration and increased significantly at 6 weeks compared with NS group (17.7±1.2)kPa vs .(15.4±1.1)kPa, P< 0. 01). Anti-ouabain antibody could significantly decrease the blood pressure of 1 k1c rats, while the normal rabbit IgG could not. Conclusion The results of this study indicate that endogenous ouabain might be one of the pathogenetic factors of hypertension.

  11. Gene Expression Analysis of Human Vascular Endothelial Cells Treated by Ouabain in Pathological Concentration

    Institute of Scientific and Technical Information of China (English)

    任延平; 吕卓人

    2004-01-01

    Objectives To study the gene expression of human vascular endothelial cells (HUVEC) treated by ouabain in pathological concentration. Methods The response of endothelial cells to ouabain of 1.8 nmol/L was explored with a complementary DNA microarray representing 8 464 different human genes. Results The results of mRNA profiles analysis indicated that 129 of the genes were differently expressed, 26 were upregulated. Conclusions The pathological role of ouabain on HUVEC may be involved in the controlling of DNA transcription、protein translation、 metabolism and signal transduction.

  12. 哇巴因对大鼠心肌重构的作用%The effects of ouabain on myocardic remodeling in the rats

    Institute of Scientific and Technical Information of China (English)

    任延平; 张明娟; 张亭; 黄若文

    2012-01-01

    Objective To study the effects of ouabain on myocardic remodeling in the rats. ' Methods Twenty-two adult male Sprague-Dawly (SD) rats were randomly divided into two groups: ouabain group (n = 12 which intraper-itoneal injection with ouabain [20 μg/(kg ? d)], control group(n=10) which intraperitoneal injection with physio-logical saline [1 mL/(kg ? d)] for 8 weeks. After 6 weeks, rats in ouabain group were divided into "ouabain-sensi-tive rats" [systolic blood pressurel (SBP) were increased, n=10] and "ouabain-resistant rats" (no significant difference in SBP, n = 2). After 8 weeks, the ultrastructural changes of myocardium were observed,by electron microscope. . The expression of mRNA of voltage-gated potassium channel 4. 2 (Kv4. 2) were detected by real-time quantitative reverse transcripition-polymerase chain reaction (RT-PCR). And the effects of ouabain on myocardia action potential and transient outward potassium current (Ito) were measured by patch clamp. Resuits After 6 weeks continued infusion, the SBP of the ouabain-sensitive rats were markedly higher than those of the control group [(138. 2 + 8. 0) vs (120. 1 + 5. 2) mm Hg, P0. 05]. There were some significant ultrastructure pathological changes including mitochondrial swelling in the mid-myocardium of apex in the,ouabain-sensitive rats. Kv4. 2 expression had no difference among the ouabain-sensitive, ouabain-resistant rats and control rats. The clamp patch results showed that ouabain could prolong action potential duration (APD90), decrease Ito, and trigger e-lectrical remodeling of myocardium. Conclusion Ouabain can increase the blood pressure, trigger cardiac structural and electrical remodeling.%目的 研究哇巴因对大鼠心肌重构的作用.方法 雄性Sprague-Dawly (SD)大鼠22只随机分为哇巴因组(n=12)及对照组(n=10),经腹腔注射哇巴因[20 μg/(kg·d)]和生理盐水[1 mL/(kg·d)]8周构建动物模型.第6周,根据收缩压情况,将哇巴因组分为哇巴

  13. Ouabain Contributes to Kidney Damage in a Rat Model of Renal Ischemia-Reperfusion Injury

    Directory of Open Access Journals (Sweden)

    Luca Villa

    2016-10-01

    Full Text Available Warm renal ischemia performed during partial nephrectomy has been found to be associated with kidney disease. Since endogenous ouabain (EO is a neuro-endocrine hormone involved in renal damage, we evaluated the role of EO in renal ischemia-reperfusion injury (IRI. We measured plasma and renal EO variations and markers of glomerular and tubular damage (nephrin, KIM-1, Kidney-Injury-Molecule-1, α1 Na-K ATPase and the protective effect of the ouabain inhibitor, rostafuroxin. We studied five groups of rats: (1 normal; (2 infused for eight weeks with ouabain (30 µg/kg/day, OHR or (3 saline; (4 ouabain; or (5 saline-infused rats orally treated with 100 µg/kg/day rostafuroxin for four weeks. In group 1, 2–3 h after IRI, EO increased in ischemic kidneys while decreased in plasma. Nephrin progressively decreased and KIM-1 mRNA increased starting from 24 h. Ouabain infusion (group 2 increased blood pressure (from 111.7 to 153.4 mmHg and ouabain levels in plasma and kidneys. In OHR ischemic kidneys at 120 h from IRI, nephrin, and KIM-1 changes were greater than those detected in the controls infused with saline (group 3. All these changes were blunted by rostafuroxin treatment (groups 4 and 5. These findings support the role of EO in IRI and suggest that rostafuroxin pre-treatment of patients before partial nephrectomy with warm ischemia may reduce IRI, particularly in those with high EO.

  14. Ouabain Contributes to Kidney Damage in a Rat Model of Renal Ischemia-Reperfusion Injury

    Science.gov (United States)

    Villa, Luca; Buono, Roberta; Ferrandi, Mara; Molinari, Isabella; Benigni, Fabio; Bettiga, Arianna; Colciago, Giorgia; Ikehata, Masami; Messaggio, Elisabetta; Rastaldi, Maria Pia; Montorsi, Francesco; Salonia, Andrea; Manunta, Paolo

    2016-01-01

    Warm renal ischemia performed during partial nephrectomy has been found to be associated with kidney disease. Since endogenous ouabain (EO) is a neuro-endocrine hormone involved in renal damage, we evaluated the role of EO in renal ischemia-reperfusion injury (IRI). We measured plasma and renal EO variations and markers of glomerular and tubular damage (nephrin, KIM-1, Kidney-Injury-Molecule-1, α1 Na-K ATPase) and the protective effect of the ouabain inhibitor, rostafuroxin. We studied five groups of rats: (1) normal; (2) infused for eight weeks with ouabain (30 µg/kg/day, OHR) or (3) saline; (4) ouabain; or (5) saline-infused rats orally treated with 100 µg/kg/day rostafuroxin for four weeks. In group 1, 2–3 h after IRI, EO increased in ischemic kidneys while decreased in plasma. Nephrin progressively decreased and KIM-1 mRNA increased starting from 24 h. Ouabain infusion (group 2) increased blood pressure (from 111.7 to 153.4 mmHg) and ouabain levels in plasma and kidneys. In OHR ischemic kidneys at 120 h from IRI, nephrin, and KIM-1 changes were greater than those detected in the controls infused with saline (group 3). All these changes were blunted by rostafuroxin treatment (groups 4 and 5). These findings support the role of EO in IRI and suggest that rostafuroxin pre-treatment of patients before partial nephrectomy with warm ischemia may reduce IRI, particularly in those with high EO. PMID:27754425

  15. Lack of ability of trypsin-treated mitochondrial F1-ATPase to bind the oligomycin-sensitivity conferring protein (OSCP).

    Science.gov (United States)

    Hundal, T; Norling, B; Ernster, L

    1983-10-03

    Soluble beef-heart mitochondrial F1-ATPase modified in its alpha-subunit by mild trypsin treatment (alpha'-F1) can no longer bind oligomycin-sensitivity conferring protein (OSCP) but is still capable of binding to F1-depleted submitochondrial particles, giving rise to a maximally oligomycin-sensitive ATPase, provided the particles contain their native complement of OSCP. When OSCP is removed from the particles, alpha'-F1 can still bind to the particles, but added OSCP induces only a low degree of oligomycin sensitivity. The possible role of OSCP in the functional coupling of the catalytic (F1) and H+-translocating (Fo) moieties of mitochondrial ATPase is discussed. The results suggest a functional similarity between the OSCP component of mitochondrial ATPase and the delta-subunit of E. coli ATPase, which is in accordance with the structural homology recently found to exist between the two polypeptides.

  16. Interdependence of ion transport and the action of ouabain in heart muscle

    Science.gov (United States)

    Bentfeld, M.; Lüllmann, H.; Peters, T.; Proppe, D.

    1977-01-01

    1 The influence of ouabain (0.4 μM) on contractile force and cellular Na and K concentrations was investigated in isolated left atria of the guinea-pig at rest and at different beat frequencies. Simultaneously the binding of ouabain to the tissue was determined. 2 Strict dependence of rates of onset of positive iontropic action and of binding of ouabain on beat frequency are limited to conditions where no alterations of cellular Na and K concentrations occur. A correlation was observed between sodium flux per unit time and the development of positive inotropism and binding to the receptors of ouabain. 3 Ouabain exerts its positive inotropic effect without affecting the intracellular Na and K concentrations in spite of the fact that under these conditions even the majority of binding sites, i.e. Na-K-adenosine triphosphatases (Na-K-ATPases), are occupied by the drug. The positive inotropic effect may be explained by a ouabain-induced conformational alteration of the Na-K-ATPase which leads to structural alterations of the plasmalemma connected with an increased availability of coupling calcium. 4 Increasing the frequency of stimulation over a critical value, which appears to be determined by an overloading of the Na pump, induces a decrease in contractile force, cellular accumulation of Na and loss of K, and eventually contracture. 5 The rate of binding of ouabain appears to depend on the actual concentration of particular conformations of the Na-K-ATPase with high affinity for ouabain. These conformations transiently occur during a pumping cycle and their concentration may therefore be dependent on the frequency of cycling which in turn is determined by the frequency of contraction. 6 Ouabain can easily be washed out from the tissue irrespective of the condition of the muscle. If, however, the intracellular Na and K homeostasis is impaired, the inhibition of the pump persists even if ouabain is released from the binding sites upon wash-out. It is suggested that

  17. Ouabain-induced internalization and lysosomal degradation of the Na+/K+-ATPase.

    Science.gov (United States)

    Cherniavsky-Lev, Marina; Golani, Ofra; Karlish, Steven J D; Garty, Haim

    2014-01-10

    Internalization of the Na(+)/K(+)-ATPase (the Na(+) pump) has been studied in the human lung carcinoma cell line H1299 that expresses YFP-tagged α1 from its normal genomic localization. Both real-time imaging and surface biotinylation have demonstrated internalization of α1 induced by ≥100 nm ouabain which occurs in a time scale of hours. Unlike previous studies in other systems, the ouabain-induced internalization was insensitive to Src or PI3K inhibitors. Accumulation of α1 in the cells could be augmented by inhibition of lysosomal degradation but not by proteosomal inhibitors. In agreement, the internalized α1 could be colocalized with the lysosomal marker LAMP1 but not with Golgi or nuclear markers. In principle, internalization could be triggered by a conformational change of the ouabain-bound Na(+)/K(+)-ATPase molecule or more generally by the disruption of cation homeostasis (Na(+), K(+), Ca(2+)) due to the partial inhibition of active Na(+) and K(+) transport. Overexpression of ouabain-insensitive rat α1 failed to inhibit internalization of human α1 expressed in the same cells. In addition, incubating cells in a K(+)-free medium did not induce internalization of the pump or affect the response to ouabain. Thus, internalization is not the result of changes in the cellular cation balance but is likely to be triggered by a conformational change of the protein itself. In physiological conditions, internalization may serve to eliminate pumps that have been blocked by endogenous ouabain or other cardiac glycosides. This mechanism may be required due to the very slow dissociation of the ouabain·Na(+)/K(+)-ATPase complex.

  18. The effect of N6-Cyclopentyladenosine on ouabain-Induced toxicity: isolated guinea pig atria

    Directory of Open Access Journals (Sweden)

    Bakhtiarian A

    2009-04-01

    Full Text Available "nBackground: Adenosine receptors play an important role in the treatment of paroxysmal supraventricular tachycardia in cardiovascular system. This effect is through interaction with A1 type of G-protein-coupled adenosine receptors. The effect of N6-cyclopentyladenosine (CPA, an A1-selective adenosine agonist, was studied on ouabain-induced toxicity in spontaneously beating isolated guinea pig atria. "nMethods: In the beginning the isolated guinea pig atria were mounted on the organ bath containing modified krebs and contractile responses in the four groups (shame, CPA, ouabain, CPA- ouabain were measured. "nResults: CPA (2-16nM produced a dose-dependent decrease in the force of contractions (34%-51% and in the rate of contractions (22%-48%. CPA significantly increased the time of onset of arrhythmia (toxicity induced by ouabain (1.2µM when it was administered 10 min before ouabain was added in organ bath. Ouabain (1.2µM alone produced arrhythmia at 7 min and either asystole or standstill at 22 min. CPA (8nM increased the time required to produce arrhythmia to 27.5 min and prolonged beating atria to more than 63 min and prevented the occurrence of asystole. "nConclusion: CPA produces direct cardiac action, probably due the inhibition of cardiac Ca2+ channel and membrane hyperpolarization of atrium cells in guinea pig atria. Moreover, our results suggest that CPA may reduce the membrane conduction through inhibition of ionic channels, which decrease ouabain- induced toxicity.

  19. Neurotensin decreases high affinity [3H]-ouabain binding to cerebral cortex membranes.

    Science.gov (United States)

    Rosin, Carina; Ordieres, María Graciela López; Arnaiz, Georgina Rodríguez de Lores

    2011-12-10

    Previous work from this laboratory showed the ability of neurotensin to inhibit synaptosomal membrane Na(+), K(+)-ATPase activity, the effect being blocked by SR 48692, a non-peptidic antagonist for high affinity neurotensin receptor (NTS1) [López Ordieres and Rodríguez de Lores Arnaiz 2000; 2001]. To further study neurotensin interaction with Na(+), K(+)-ATPase, peptide effect on high affinity [(3)H]-ouabain binding was studied in cerebral cortex membranes. It was observed that neurotensin modified binding in a dose-dependent manner, leading to 80% decrease with 1 × 10(-4)M concentration. On the other hand, the single addition of 1 × 10(-6)M, 1 × 10(-5)M and 1 × 10(-4)M SR 48692 (Sanofi-Aventis, U.S., Inc.) decreased [(3)H]-ouabain binding (in %) to 87 ± 16; 74 ± 16 and 34 ± 17, respectively. Simultaneous addition of neurotensin and SR 48692 led to additive or synergic effects. Partial NTS2 agonist levocabastine inhibited [(3)H]-ouabain binding likewise. Saturation assays followed by Scatchard analyses showed that neurotensin increased K(d) value whereas failed to modify B(max) value, indicating a competitive type interaction of the peptide at Na(+), K(+)-ATPase ouabain site. At variance, SR 48692 decreased B(max) value whereas it did not modify K(d) value. [(3)H]-ouabain binding was also studied in cerebral cortex membranes obtained from rats injected i. p. 30 min earlier with 100 μg and 250 μg/kg SR 48692. It was observed that the 250 μg/kg SR 48692 dose led to 19% decrease in basal [(3)H]-ouabain binding. After SR 48692 treatments, addition of 1 × 10(-6)M led to additive or synergic effect. Results suggested that [(3)H]-ouabain binding inhibition by neurotensin hardly involves NTS1 receptor.

  20. Mps1 Mediated Phosphorylation of Hsp90 Confers Renal Cell Carcinoma Sensitivity and Selectivity to Hsp90 Inhibitors

    Directory of Open Access Journals (Sweden)

    Mark R. Woodford

    2016-02-01

    Full Text Available The molecular chaperone Hsp90 protects deregulated signaling proteins that are vital for tumor growth and survival. Tumors generally display sensitivity and selectivity toward Hsp90 inhibitors; however, the molecular mechanism underlying this phenotype remains undefined. We report that the mitotic checkpoint kinase Mps1 phosphorylates a conserved threonine residue in the amino-domain of Hsp90. This, in turn, regulates chaperone function by reducing Hsp90 ATPase activity while fostering Hsp90 association with kinase clients, including Mps1. Phosphorylation of Hsp90 is also essential for the mitotic checkpoint because it confers Mps1 stability and activity. We identified Cdc14 as the phosphatase that dephosphorylates Hsp90 and disrupts its interaction with Mps1. This causes Mps1 degradation, thus providing a mechanism for its inactivation. Finally, Hsp90 phosphorylation sensitizes cells to its inhibitors, and elevated Mps1 levels confer renal cell carcinoma selectivity to Hsp90 drugs. Mps1 expression level can potentially serve as a predictive indicator of tumor response to Hsp90 inhibitors.

  1. The Effect of Paroxetine on Ouabain-Induced Toxicity in Isolated Guinea Pig Atria

    Directory of Open Access Journals (Sweden)

    Farzaneh Najar

    2011-04-01

    Full Text Available It has been reported that selective serotonin reuptake inhibitors (SSRIs possess some cardiac effects. In the present study we have investigated the effect of paroxetine (PX, a potent SSRI agent, on spontaneously as well as ouabain-induced arrhythmia beating isolated guinea-pig atria. The Guinea-pig heart was rapidly removed; the auricles were dissected out in oxygenated modified Krebs solution. The rate and force of spontaneous contractions were recorded isometrically with a photosensitive transducer. PX (1-16 µg/ml caused a dose-dependent decrease in the rate of contractions (14-70% and contractile force (8-16%. Ouabain alone (1.2 µg/ml produced arrhythmia at 7.2 ± 1.5 min and asystole at 20.1 ± 3.1 min. Pretreatment with PX (4 µg/ml significantly increased the time of arrhythmia onset to 19.8 min. In addition, PX prolonged the duration of action beating from 20.1 ± 3.1 min to 43.1± 2.6 and delayed the occurrence of asystole. The pattern of contractile force by PX + ouabain treatment was more regular than that observed after administration of ouabain alone. The above findings may the probably be due to the inhibition of cardiac Na+ and Ca2+ channels or autonomic nervous system. Results also suggest that PX may reduce the membrane conductance through inhibition of ionic channels to prevent ouabain-induced arrhythmia.

  2. Decreased BDNF levels in amygdala and hippocampus after intracerebroventricular administration of ouabain

    Directory of Open Access Journals (Sweden)

    Luciano K. Jornada

    2012-01-01

    Full Text Available OBJECTIVE: The present study aims to investigate the effects of ouabain intracerebroventricular injection on BDNF levels in the amygdala and hippocampus of Wistar rats. METHODS: Animals received a single intracerebroventricular injection of ouabain (10-3 and 10-2 M or artificial cerebrospinal fluid and immediately, 1h, 24h, or seven days after injection, BDNF levels were measured in the rat's amygdala and hippocampus by sandwich-ELISA (n = 8 animals per group. RESULTS: When evaluated immediately, 3h, or 24h after injection, ouabain in doses of 10-2 and 10-3 M does not alter BDNF levels in the amygdala and hippocampus. However, when evaluated seven days after injection, ouabain in 10-2 and 10-3 M, showed a significant reduction in BDNF levels in both brain regions evaluated. DISCUSSION: In conclusion, we propose that the ouabain decreased BDNF levels in the hippocampus and amygdala when assessed seven days after administration, supporting the Na/K ATPase hypothesis for bipolar illness.

  3. The alkaloid matrine of the root of Sophora flavescens prevents arrhythmogenic effect of ouabain.

    Science.gov (United States)

    Zhou, Yuhong; Wu, Yun; Deng, Lin; Chen, Lanlan; Zhao, Dandan; Lv, Lifang; Chen, Xu; Man, Jinyu; Wang, Yansong; Shan, Hongli; Lu, Yanjie

    2014-06-15

    Matrine, a alkaloid of the root of Sophora flavescens, has multiple protective effects on the cardiovascular system including cardiac arrhythmias. However, the molecular and ionic mechanisms of matrine have not been well investigated. Our study aimed at to shed a light on the issue to investigate the antiarrhythmic effects of matrine by using ouabain to construct an arrhythmic model of cardiomyocytes. In this experiment, matrine significantly and dose-dependently increased the doses of ouabain required to induce cardiac arrhythmias and decreased the duration of arrhythmias in guinea pigs. In cardiomyocytes of guinea pigs, ouabain 10 μM prolonged action potential duration by 80% (pMatrine 100 μM shortened the prolongation of APD and prevented the increase of L-type Ca(2+) currents and Ca(2+) transients induced by ouabain. Taken together, these findings provide the first evidence that matrine possessed arrhythmogenic effect of ouabain by inhibiting of L-type Ca(2+) currents and Ca(2+) overload in guinea pigs.

  4. Broadband sensitized photon up-conversion at subsolar irradiance (Conference Presentation)

    Science.gov (United States)

    Pedrini, Jacopo; Monguzzi, Angelo; Meinardi, Francesco

    2016-09-01

    A crucial limit of solar devices is their inability to harvest the full solar spectrum. Currently, sensitized up-conversion based on triplet-tripled annihilation (STTA-UC) in bi-component organic systems is the most promising technique to recover sub-bandgap photons, showing good efficiencies also at excitation intensities comparable to the solar irradiance. In STTA-UC, high-energy light is generated through annihilation of metastable triplet states of molecules acting as emitters, which are populated via resonant energy transfer from a light-harvesting sensitizer. However, suitable sensitizers show narrow absorption bands, limiting the fraction of recoverable photons, therefore preventing the application of STTA-UC to real-world devices. Here we demonstrate how to overcome the described limit by using multiple sensitizers that work cooperatively to broaden the overall system absorption band. This is obtained using an additional sensitizer that transfers the extra harvested energy to the main one (sensitization of the sensitizer), or a set of properly designed complementary absorbing sensitizers all able to excite simultaneously the same emitter (multi-sensitizers). In both cases STTA-UC performances result strongly enhanced compared to the corresponding mono-sensitizer system, increasing the up-converted light intensity generated at AM 1.5 up to two times. Remarkably, by coupling our light converters to a DSSC we prove its operation by exploiting exclusively sub-bandgap photons. A detailed modeling of the photophysical processes involved in these complex systems allows us to draw the guidelines for the design of the next generation STTA-UC materials, encouraging their application to photovoltaic technologies.

  5. Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast

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    Kozmin Stanislav G

    2005-06-01

    Full Text Available Abstract Background N-hydroxylated base analogs, such as 6-hydroxylaminopurine (HAP and 2-amino-6-hydroxylaminopurine (AHA, are strong mutagens in various organisms due to their ambiguous base-pairing properties. The systems protecting cells from HAP and related noncanonical purines in Escherichia coli include specialized deoxyribonucleoside triphosphatase RdgB, DNA repair endonuclease V, and a molybdenum cofactor-dependent system. Fewer HAP-detoxification systems have been identified in yeast Saccharomyces cerevisiae and other eukaryotes. Cellular systems protecting from AHA are unknown. In the present study, we performed a genome-wide search for genes whose deletions confer sensitivity to HAP and AHA in yeast. Results We screened the library of yeast deletion mutants for sensitivity to the toxic and mutagenic action of HAP and AHA. We identified novel genes involved in the genetic control of base analogs sensitivity, including genes controlling purine metabolism, cytoskeleton organization, and amino acid metabolism. Conclusion We developed a method for screening the yeast deletion library for sensitivity to the mutagenic and toxic action of base analogs and identified 16 novel genes controlling pathways of protection from HAP. Three of them also protect from AHA.

  6. [3H]ouabain binding to cultured rat vascular smooth muscle cells.

    Science.gov (United States)

    Khalil, F; Hopp, L; Searle, B M; Tokushige, A; Tamura, H; Kino, M; Aviv, A

    1984-05-01

    The number of Na+ pump units (Bmax) and the equilibrium dissociation constant (Kd) for ouabain as well as parameters of K+ binding to the Na+ pump were examined in in vitro-grown vascular smooth muscle cells ( VSMC ) derived from Sprague-Dawley rats. The technique to measure these variables utilizes analyses of [3H]ouabain displacement from its VSMC receptors by nonlabeled ouabain and K+. The mean values for Bmax and Kd in the cultured VSMCs were 1.95 X 10(5) receptor sites per single VSMC and 2.68 X 10(-6) M, respectively. The equilibrium dissociation constant for K+ (Ki) was 0.92 mM. K+ binding to the cultured VSMCs demonstrated positive cooperativity with a Hill coefficient (n) of 1.78.

  7. A kinetic study of the ouabain-induced efflux of norepinephrine from the dog saphenous vein

    Energy Technology Data Exchange (ETDEWEB)

    Monteiro, J.G. (Laboratorio de Farmacologia, Faculdade de Medicina, Porto (Portugal))

    1991-07-01

    Dog saphenous vein strips were incubated with (3H)norepinephrine ((3H)NE), 1.4 microM, after inhibition of the NE-metabolizing enzymes and extraneuronal uptake, and superfused for up to 290 min. From the 70th min onwards the strips were exposed to 10 microM ouabain, some of them being subject to electrical stimulation from the 140th min onwards. Other strips were exposed to either 1, 10 or 100 microM ouabain from the 70th min onwards. The spontaneous efflux of (3H)NE had a long half-time (156 min), and over 90% of the (3H)NE accumulated did not participate in efflux (bound fraction). Ouabain, 10 microM, induced a pronounced increase of the rate of efflux of (3H)NE, which was delayed in its onset and reached a maximum at t = 135 min of superfusion. Increasing the concentration of ouabain decreased both the delay to the beginning of the overflow and the time to maximum efflux and increased the maximum rate of efflux. In Ca(++)-free medium (during the superfusion period), the maximum rate of efflux was lower than in Ca(++)-containing medium, but was attained earlier. The bound fraction amounted to 22% when the efflux was induced by 10 microM ouabain in Ca(++)-containing medium, a value unnaffected by electrical stimulation but reduced markedly by omitting calcium. The results support the view that the efflux of (3H)NE induced by ouabain is delayed and that it is both carrier-mediated and due to exocytosis.

  8. Age-dependent decrease in the hepatic uptake and biliary excretion of ouabain in rats.

    Science.gov (United States)

    Ohta, M; Kanai, S; Sato, Y; Kitani, K

    1988-03-01

    The biliary excretion of i.v. injected ouabain was examined in male and female Wistar-derived rats in relation to age. The hepatic uptake velocity for ouabain was also determined in isolated hepatocyte preparations obtained from male rats of various ages. Biliary recovery values of ouabain (percent of the dose) were fairly comparable for young male and female rats (3-4 month old). Recovery progressively decreased with age, the first 10-min recoveries at 24 months being about one-third those of respective young values in both sexes. A significant linear relation was demonstrated between the first 10-min recovery (Y, percent of the dose) and rat age (X, month), yielding the relations of Y = 17.75-0.43X for males and Y = 18.99-0.43X for females respectively. Similarly, the initial uptake velocity (Y, nmol/mg/min) for ouabain decreased in a linear fashion with age (X, month), yielding a significant negative correlation (Y = 0.704-0.0021X, r = -0.839, P less than 0.005, N = 21) at an ouabain concentration of 8 microM. Kinetic studies using non-linear regression analysis revealed a significantly lower Vmax value (0.533 +/- 0.041 nmol/mg/min) in old (24-29 months) rats compared to the young (4-4.5 months) value (1.193 +/- 0.105 nmol per mg/min, P less than 0.05), while the affinity constant (Km, microM) did not differ significantly between young and old animals (203.12 +/- 25.42 microM in young rats vs 283.68 +/- 28.90 microM in old rats, mean +/- SE, 0.05 less than P less than 0.1). The results of the present study suggest that the age-dependent decrease in the biliary recovery of i.v. injected ouabain in rats can be largely explained by the decrease with age in the hepatic uptake of ouabain. Furthermore, the results provide further support for our previous thesis that the decrease in the lateral mobility of hepatocyte plasma membrane proteins, as revealed by the fluorescence recovery after photobleaching technique, may play a significant role in the age

  9. Design of a sensitive grating-based phase contrast mammography prototype (Conference Presentation)

    Science.gov (United States)

    Arboleda Clavijo, Carolina; Wang, Zhentian; Köhler, Thomas; van Stevendaal, Udo; Martens, Gerhard; Bartels, Matthias; Villanueva-Perez, Pablo; Roessl, Ewald; Stampanoni, Marco

    2017-03-01

    Grating-based phase contrast mammography can help facilitate breast cancer diagnosis, as several research works have demonstrated. To translate this technique to the clinics, it has to be adapted to cover a large field of view within a limited exposure time and with a clinically acceptable radiation dose. This indicates that a straightforward approach would be to install a grating interferometer (GI) into a commercial mammography device. We developed a wave propagation based optimization method to select the most convenient GI designs in terms of phase and dark-field sensitivities for the Philips Microdose Mammography (PMM) setup. The phase sensitivity was defined as the minimum detectable breast tissue electron density gradient, whereas the dark-field sensitivity was defined as its corresponding signal-to-noise Ratio (SNR). To be able to derive sample-dependent sensitivity metrics, a visibility reduction model for breast tissue was formulated, based on previous research works on the dark-field signal and utilizing available Ultra-Small-Angle X-ray Scattering (USAXS) data and the outcomes of measurements on formalin-fixed breast tissue specimens carried out in tube-based grating interferometers. The results of this optimization indicate the optimal scenarios for each metric are different and fundamentally depend on the noise behavior of the signals and the visibility reduction trend with respect to the system autocorrelation length. In addition, since the inter-grating distance is constrained by the space available between the breast support and the detector, the best way we have to improve sensitivity is to count on a small G2 pitch.

  10. Growth and immunity conferred by a Plasmodium falciparum temperature sensitive mutant in Panamanian owl monkeys.

    Science.gov (United States)

    Inselburg, J; Rossan, R N; Escajadillo, A

    1989-05-01

    We have compared the growth of the wild type Plasmodium falciparum strain Honduras 1 and a previously isolated temperature sensitive mutant of it, AP1-16, in Panamanian owl monkeys. We examined serially infected splenectomized and normal animals that were initially infected with cultured parasites that had been grown in a mixture of owl monkey and human erythrocytes. Initial infections in splenectomized monkeys were marked by multiple recrudescences. The mutant grew less well than the wild type in the splenectomized monkeys, as determined by lower peak and total parasitemias. In the splenectomized monkeys tested by rechallenge with the wild type parasite, the mutant stimulated a comparable degree of protection. That protection was manifested in 2 ways. There was a marked reduction in the level of the primary parasitemia in the rechallenged monkeys and an absence of recrudescent parasitemias after the primary parasitemia. The potential value of generating and studying temperature sensitive P. falciparum strains that show attenuated growth is considered.

  11. Distress of ostracism: oxytocin receptor gene polymorphism confers sensitivity to social exclusion

    OpenAIRE

    McQuaid, Robyn J.; McInnis, Opal A.; Matheson, Kimberly; Anisman, Hymie

    2015-01-01

    A single-nucleotide polymorphism on the oxytocin receptor gene (OXTR), rs53576, involving a guanine (G) to adenine (A) substitution has been associated with altered prosocial features. Specifically, individuals with the GG genotype (i.e. the absence of the polymorphism) display beneficial traits including enhanced trust, empathy and self-esteem. However, because G carriers might also be more socially sensitive, this may render them more vulnerable to the adverse effects of a negative social s...

  12. Distress of ostracism: oxytocin receptor gene polymorphism confers sensitivity to social exclusion.

    Science.gov (United States)

    McQuaid, Robyn J; McInnis, Opal A; Matheson, Kimberly; Anisman, Hymie

    2015-08-01

    A single-nucleotide polymorphism on the oxytocin receptor gene (OXTR), rs53576, involving a guanine (G) to adenine (A) substitution has been associated with altered prosocial features. Specifically, individuals with the GG genotype (i.e. the absence of the polymorphism) display beneficial traits including enhanced trust, empathy and self-esteem. However, because G carriers might also be more socially sensitive, this may render them more vulnerable to the adverse effects of a negative social stressor. The current investigation, conducted among 128 white female undergraduate students, demonstrated that relative to individuals with AA genotype, G carriers were more emotionally sensitive (lower self-esteem) in response to social ostracism promoted through an on-line ball tossing game (Cyberball). Furthermore, GG individuals also exhibited altered blood pressure and cortisol levels following rejection, effects not apparent among A carriers. The data support the view that the presence of the G allele not only promotes prosocial behaviors but also favors sensitivity to a negative social stressor.

  13. Elevated glutathione levels confer cellular sensitization to cisplatin toxicity by up-regulation of copper transporter hCtr1.

    Science.gov (United States)

    Chen, Helen H W; Song, Im-Sook; Hossain, Anwar; Choi, Min-Koo; Yamane, Yoshiaki; Liang, Zheng D; Lu, Jia; Wu, Lily Y-H; Siddik, Zahid H; Klomp, Leo W J; Savaraj, Niramol; Kuo, Macus Tien

    2008-09-01

    Previous studies have demonstrated that treating cultured cells with cisplatin (CDDP) up-regulated the expression of glutathione (GSH) and its de novo rate-limiting enzyme glutamate-cysteine ligase (GCL), which consists of a catalytic (GCLC) and a modifier (GCLM) subunit. It has also been shown that many CDDP-resistant cell lines exhibit high levels of GCLC/GCLM and GSH. Because the GSH system is the major intracellular regulator of redox conditions that serve as an important detoxification cytoprotector, these results have been taken into consideration that elevated levels of GCL/GSH are responsible for the CDDP resistance. In contrast to this context, we demonstrated here that overexpression of GSH by transfection with an expression plasmid containing the GCLC cDNA conferred sensitization to CDDP through up-regulation of human copper transporter (hCtr) 1, which is also a transporter for CDDP. Depleting GSH levels in these transfected cells reversed CDDP sensitivity with concomitant reduction of hCtr1 expression. Although rates of copper transport were also up-regulated in the transfected cells, these cells exhibited biochemical signature of copper deficiency, suggesting that GSH functions as an intracellular copper-chelator and that overexpression of GSH can alter copper metabolism. More importantly, our results reveal a new role of GSH in the regulation of CDDP sensitivity. Overproduction of GSH depletes the bioavailable copper pool, leading to up-regulation of hCtr1 and sensitization of CDDP transport and cell killing. These findings also have important implications in that modulation of the intracellular copper pool may be a novel strategy for improving chemotherapeutic efficacy of platinum-based antitumor agents.

  14. Increased number of ouabain binding sites in lymphocytes from borderline hypertensives

    DEFF Research Database (Denmark)

    Nielsen, J R; Pedersen, K E; Klitgaard, N A;

    1989-01-01

    triglyceride, and serum cholesterol, which may influence the number of ouabain binding sites. Only BMI entered the stepwise model. These results indicate the presence of an increased number of sodium-potassium pumps in lymphocytes from borderline hypertensives. This difference may be attributed to the blood...

  15. Vasoconstriction induced by ouabain in the canine coronary artery: contribution of adrenergic and nonadrenergic responses.

    Science.gov (United States)

    Cooke, J P; Shepherd, J T; Vanhoutte, P M

    1988-07-01

    Ouabain, when applied to rings of the left circumflex coronary artery of the dog (which contains both alpha 1-adrenoceptors leading to contraction and beta 1-adrenoceptors leading to relaxation) caused an initial contraction which peaked within 15 minutes and a later secondary increase in tension which peaked within 60 minutes. These contractions were prevented by Ca2+ removal or by verapamil. Adrenergic denervation with 6-hydroxydopamine did not affect the initial contraction. Thus it is due to a nonadrenergic effect of the glycoside. Since the secondary increase in tension was prevented by adrenergic denervation and prazosin, it is likely to be due to norepinephrine released from adrenergic nerves acting on alpha-adrenoceptors. This interpretation was confirmed by the finding that ouabain, after a latent period of about 35 minutes, augmented the output of 3H-norepinephrine from helical strips of the artery previously incubated with tritiated transmitter. In rings contracted with prostaglandin F2 alpha, ouabain reduced beta-adrenergic relaxations caused by isoproterenol or exogenous norepinephrine, but not those caused by sodium nitroprusside. Thus, in this artery, ouabain depresses the responses of the beta-adrenoceptors to the norepinephrine which it releases, thereby permitting the neurotransmitter to cause contraction by activating postjunctional alpha 1-adrenoceptors.

  16. Protective effect of eicosapentaenoic acid on ouabain toxicity in neonatal rat cardiac myocytes

    Energy Technology Data Exchange (ETDEWEB)

    Hallaq, H.; Leaf, A. (Harvard Medical School, Boston, MA (USA)); Sellmayer, A. (Univ. Munchen, (Germany)); Smith, T.W. (Brigham and Women' s Hospital, Boston, MA (USA))

    1990-10-01

    Isolated neonatal cardiac myocytes have been utilized as a model for the study of cardiac arrhythmogenic factors. The myocytes respond to the toxic effects of a potent cardiac glycoside, ouabain at 0.1 mM, by an increase in their spontaneous beating rate and a reduction in amplitude of contractions resulting within minutes in a lethal state of contracture. Incubating the isolated myocytes for 3{endash}5 days in culture medium enriched with 5 {mu}M arachidonic acid had no effect on the development of lethal contracture after subsequent exposure to 0.1 mM ouabain. By contrast, incubating the myocytes for 3{endash}5 days with 5 {mu}M eicosapentaenoic acid completely prevented the toxic effects of ouabain at 0.1 mM. No differences in bumetanide-inhibitable {sup 86}Rb flux were observed between the three preparations. However, measurements with fura-2 of cytosolic free calcium levels indicated that control and arachidonic acid-enriched myocytes developed toxic cytosolic calcium concentrations of 845 {plus minus} 29 and 757 {plus minus} 64 nM, respectively, on exposure to 0.1 mM ouabain, whereas in eicosapentaenoic acid-enriched myocytes, physiologic calcium levels were preserved. Incubating the myocytes with eicosapentaenoic acid for 3{endash}5 days resulted in a small reduction of arachidonic acid and a small but significant increase of eicosapentaenoic acid in membrane phospolipids of the myocytes.

  17. Industrial femtosecond lasers for machining of heat-sensitive polymers (Conference Presentation)

    Science.gov (United States)

    Hendricks, Frank; Bernard, Benjamin; Matylitsky, Victor V.

    2017-03-01

    Heat-sensitive materials, such as polymers, are used increasingly in various industrial sectors such as medical device manufacturing and organic electronics. Medical applications include implantable devices like stents, catheters and wires, which need to be structured and cut with minimum heat damage. Also the flat panel display market moves from LCD displays to organic LED (OLED) solutions, which utilize heat-sensitive polymer substrates. In both areas, the substrates often consist of multilayer stacks with different types of materials, such as metals, dielectric layers and polymers with different physical characteristic. The different thermal behavior and laser absorption properties of the materials used makes these stacks difficult to machine using conventional laser sources. Femtosecond lasers are an enabling technology for micromachining of these materials since it is possible to machine ultrafine structures with minimum thermal impact and very precise control over material removed. An industrial femtosecond Spirit HE laser system from Spectra-Physics with pulse duration 120 μJ and average output powers of >16 W is an ideal tool for industrial micromachining of a wide range of materials with highest quality and efficiency. The laser offers process flexibility with programmable pulse energy, repetition rate, and pulse width. In this paper, we provide an overview of machining heat-sensitive materials using Spirit HE laser. In particular, we show how the laser parameters (e.g. laser wavelength, pulse duration, applied energy and repetition rate) and the processing strategy (gas assisted single pass cut vs. multi-scan process) influence the efficiency and quality of laser processing.

  18. High-speed high-sensitivity infrared spectroscopy using mid-infrared swept lasers (Conference Presentation)

    Science.gov (United States)

    Childs, David T. D.; Groom, Kristian M.; Hogg, Richard A.; Revin, Dmitry G.; Cockburn, John W.; Rehman, Ihtesham U.; Matcher, Stephen J.

    2016-03-01

    Infrared spectroscopy is a highly attractive read-out technology for compositional analysis of biomedical specimens because of its unique combination of high molecular sensitivity without the need for exogenous labels. Traditional techniques such as FTIR and Raman have suffered from comparatively low speed and sensitivity however recent innovations are challenging this situation. Direct mid-IR spectroscopy is being speeded up by innovations such as MEMS-based FTIR instruments with very high mirror speeds and supercontinuum sources producing very high sample irradiation levels. Here we explore another possible method - external cavity quantum cascade lasers (EC-QCL's) with high cavity tuning speeds (mid-IR swept lasers). Swept lasers have been heavily developed in the near-infrared where they are used for non-destructive low-coherence imaging (OCT). We adapt these concepts in two ways. Firstly by combining mid-IR quantum cascade gain chips with external cavity designs adapted from OCT we achieve spectral acquisition rates approaching 1 kHz and demonstrate potential to reach 100 kHz. Secondly we show that mid-IR swept lasers share a fundamental sensitivity advantage with near-IR OCT swept lasers. This makes them potentially able to achieve the same spectral SNR as an FTIR instrument in a time x N shorter (N being the number of spectral points) under otherwise matched conditions. This effect is demonstrated using measurements of a PDMS sample. The combination of potentially very high spectral acquisition rates, fundamental SNR advantage and the use of low-cost detector systems could make mid-IR swept lasers a powerful technology for high-throughput biomedical spectroscopy.

  19. Towards a high sensitivity small animal PET system based on CZT detectors (Conference Presentation)

    Science.gov (United States)

    Abbaszadeh, Shiva; Levin, Craig

    2017-03-01

    Small animal positron emission tomography (PET) is a biological imaging technology that allows non-invasive interrogation of internal molecular and cellular processes and mechanisms of disease. New PET molecular probes with high specificity are under development to target, detect, visualize, and quantify subtle molecular and cellular processes associated with cancer, heart disease, and neurological disorders. However, the limited uptake of these targeted probes leads to significant reduction in signal. There is a need to advance the performance of small animal PET system technology to reach its full potential for molecular imaging. Our goal is to assemble a small animal PET system based on CZT detectors and to explore methods to enhance its photon sensitivity. In this work, we reconstruct an image from a phantom using a two-panel subsystem consisting of six CZT crystals in each panel. For image reconstruction, coincidence events with energy between 450 and 570 keV were included. We are developing an algorithm to improve sensitivity of the system by including multiple interaction events.

  20. The trophic effect of ouabain on retinal ganglion cells is mediated by IL-1β and TNF-α

    Energy Technology Data Exchange (ETDEWEB)

    Salles von-Held-Ventura, Juliana; Mázala-de-Oliveira, Thalita; Cândida da Rocha Oliveira, Amanda; Granja, Marcelo Gomes [Departamento de Neurobiologia, Programa de Neurociências, Outeiro de São João Batista s/n CEP: 24020-150, Universidade Federal Fluminense, Niterói, RJ (Brazil); Gonçalves-de-Albuquerque, Cassiano Felippe; Castro-Faria-Neto, Hugo Caire [Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Departamento de Fisiologia e Farmacodinâmica, Av., no 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ (Brazil); Giestal-de-Araujo, Elizabeth, E-mail: egiestal@vm.uff.br [Departamento de Neurobiologia, Programa de Neurociências, Outeiro de São João Batista s/n CEP: 24020-150, Universidade Federal Fluminense, Niterói, RJ (Brazil)

    2016-09-09

    Ouabain is a steroid hormone that binds to the enzyme Na{sup +}, K{sup +} – ATPase and stimulates different intracellular pathways controlling growth, proliferation and cell survival. IL-1β and TNF-α are pleiotropic molecules, conventionally regarded as pro-inflammatory cytokines with well-known effects in the immune system. In addition, IL-1β and TNF-α also play important roles in the nervous system including neuroprotective effects. Previous data from our group showed that ouabain treatment is able to induce an increase in retinal ganglion cell survival kept in mixed retinal cell cultures. The aim of this work was to investigate if IL-1β and TNF-α could be mediating the trophic effect of ouabain on retinal ganglion cells. Our results show that the trophic effect of ouabain on retinal ganglion cell was inhibited by either anti-IL-1β or anti-TNF-α antibodies. In agreement, IL-1β or TNF-α increased the retinal ganglion cells survival in a dose-dependent manner. Accordingly, ouabain treatment induces a temporal release of TNF-α and IL-1β from retinal cell cultures. Interestingly, TNF-α and IL-1β regulate each other intracellular levels. Our results suggest that ouabain treatment triggers the activation of TNF-α and IL-1β signaling pathways leading to an increase in retinal ganglion cell survival. - Highlights: • Pro-inflammatory cytokines regulates the ouabain effect on RGC survival. • Ouabain treatment modulates the intracellular levels of TNF-α and IL-1β. • Ouabain induces the release of TNF-α and IL-1β in retinal cell cultures.

  1. Planoconcave optical microresonator sensors for photoacoustic imaging: pushing the limits of sensitivity (Conference Presentation)

    Science.gov (United States)

    Guggenheim, James A.; Zhang, Edward Z.; Beard, Paul C.

    2016-03-01

    Most photoacoustic scanners use piezoelectric detectors but these have two key limitations. Firstly, they are optically opaque, inhibiting backward mode operation. Secondly, it is difficult to achieve adequate detection sensitivity with the small element sizes needed to provide near-omnidirectional response as required for tomographic imaging. Planar Fabry-Perot (FP) ultrasound sensing etalons can overcome both of these limitations and have proved extremely effective for superficial (heating. Finally, a prototype microresonator based photoacoustic scanner has been developed and applied to the problem of deep-tissue (>1cm) photoacoustic imaging in vivo. Imaging results for second generation microresonator sensors (with R = 99.5% and thickness up to ~800um) are compared to the best achievable with the planar FP sensors and piezoelectric receivers.

  2. Upregulation of Na+ and Ca2+ transporters in arterial smooth muscle from ouabain-induced hypertensive rats

    OpenAIRE

    Pulina, Maria V.; Zulian, Alessandra; Berra-Romani, Roberto; Beskina, Olga; Mazzocco-Spezzia, Amparo; Baryshnikov, Sergey G.; Papparella, Italia; Hamlyn, John M.; Blaustein, Mordecai P.; Golovina, Vera A.

    2009-01-01

    Prolonged ouabain administration (25 μg·kg−1·day−1 for 5 wk) induces “ouabain hypertension” (OH) in rats, but the molecular mechanisms by which ouabain elevates blood pressure are unknown. Here, we compared Ca2+ signaling in mesenteric artery smooth muscle cells (ASMCs) from normotensive (NT) and OH rats. Resting cytosolic free Ca2+ concentration ([Ca2+]cyt; measured with fura-2) and phenylephrine-induced Ca2+ transients were augmented in freshly dissociated OH ASMCs. Immunoblots revealed tha...

  3. First clinical pilot study with intravascular polarization sensitive optical coherence tomography (Conference Presentation)

    Science.gov (United States)

    Villiger, Martin; Karanasos, Antonios; Ren, Jian; Lippok, Norman; Shishkov, Milen; Daemen, Joost; Van Mieghem, Nicolas; Diletti, Roberto; Valgimigli, Marco; van Geuns, Robert-Jan; de Jaegere, Peter; Zijlstra, Felix; van Soest, Gijs; Nadkarni, Seemantini; Regar, Evelyn; Bouma, Brett E.

    2016-02-01

    Polarization sensitive (PS) OCT measures the polarization states of the light backscattered by tissue and provides measures of tissue birefringence and depolarization in addition to the structural OCT signal. Ex vivo studies have demonstrated that birefringence is increased in tissue rich in collagen and with elevated smooth muscle cell content. Preliminary data further suggests that depolarization can identify regions of macrophage infiltration, lipid, and irregularly arranged collagen fibers. These are important aspects of the mechanical integrity and vulnerability of atherosclerotic plaques. To evaluate the potential of PS-OCT in the clinical setting, we combined our custom PS-OCT system with commercially available OCT catheters (Fastview, Terumo Corporation) and performed a pilot study in 30 patients, scheduled to undergo percutaneous coronary intervention (PCI) on the grounds of stable or unstable angina. A total of 82 pullbacks in 39 vessels were performed, either in the native coronary arteries or post procedure. Comparing consecutive pullbacks of the same coronary artery, we found excellent agreement between the polarization features in the repeat pullbacks, validating the repeatability and robustness of PS-OCT in the clinical in vivo setting. In addition we observed that the birefringence and depolarization features vary significantly across lesions with identical structural OCT appearance, suggesting morphological subtypes. This first human pilot study proved the feasibility and robustness of intravascular PS-OCT. PS-OCT achieves improved tissue characterization and may help in identifying high-risk plaques, with the potential to ultimately improve risk stratification and help guiding PCI.

  4. Palmitoylation of STREX domain confers cerebroside sensitivity to the BKCa channel.

    Science.gov (United States)

    Zhang, Yujiao; Zhou, Li; Zou, Jun; Wang, Mingyan; Qi, Jianhua; Qi, Zhi

    2014-10-01

    Our previous study reported that cerebrosides from traditional Chinese medicine Baifuzi directly interact with the STREX domain of BKCa channels, which in turn results in the therapeutic effect of Baifuzi on ischemic stroke. However, it is not known how cerebrosides in the plasma membrane could interact with the STREX domain that is in the cytoplasmic side. Using patch-clamp technique, effects of different cerebrosides on the BKCa channel were studied by measuring single channel currents in CHO cells expressing wild type or mutated BKCa channels. Palmitoylation of the STREX domain was removed either by site-directed mutagenesis or pharmacological inhibition. Removal of palmitoylation sites at C646 and C647 by mutating the residues to Ala abolished the ability of cerebrosides to activate the BKCa channel. In contrast, the mutation neither changed the single channel conductance nor voltage sensitivity of the channel. Both palmitoylation inhibitors tunicamycin and palmitic acid analog 2-bromopalmitate attenuated the activation of the BKCa channel by cerebrosides. Furthermore, confocal images on STREX-EGFP fragments demonstrated that STREX fragments no longer associated with the plasma membrane when the palmitoylation was removed or blocked. These findings suggest that palmitoylation of the STREX domain is necessary for cerebrosides to activate the BKCa channel and provide insight into the mechanism of how Baifuzi could exert therapeutic effect on ischemic stroke. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Screening for Escherichia coli K-12 genes conferring glyoxal resistance or sensitivity by transposon insertions.

    Science.gov (United States)

    Lee, Changhan; Kim, Jihong; Kwon, Minsuk; Lee, Kihyun; Min, Haeyoung; Kim, Seong Hun; Kim, Dongkyu; Lee, Nayoung; Kim, Jiyeun; Kim, Doyun; Ko, Changmin; Park, Chankyu

    2016-09-01

    Glyoxal (GO) belongs to the reactive electrophilic species generated in vivo in all organisms. In order to identify targets of GO and their response mechanisms, we attempted to screen for GO-sensitive mutants by random insertions of TnphoA-132. The genes responsible for GO susceptibility were functionally classified as the following: (i) tRNA modification; trmE, gidA and truA, (ii) DNA repair; recA and recC, (iii) toxin-antitoxin; mqsA and (iv) redox metabolism; yqhD and caiC In addition, an insertion in the crp gene, encoding the cAMP responsive transcription factor, exhibits a GO-resistant phenotype, which is consistent with the phenotype of adenylate cyclase (cya) mutant showing GO resistance. This suggests that global regulation involving cAMP is operated in a stress response to GO. To further characterize the CRP-regulated genes directly associated with GO resistance, we created double mutants deficient in both crp and one of the candidate genes including yqhD, gloA and sodB The results indicate that these genes are negatively regulated by CRP as confirmed by real-time RT-PCR. We propose that tRNA as well as DNA are the targets of GO and that toxin/antitoxin, antioxidant and cAMP are involved in cellular response to GO.

  6. Towards early in vivo photoacoustic malaria diagnosis with 10,000-fold sensitivity improvement (Conference Presentation)

    Science.gov (United States)

    Carey, Kai A.; Menyaev, Yulian A.; Nedosekin, Dmitry A.; Sarimollaoglu, Mustafa; Galanzha, Ekaterina I.; Stumhofer, Jason S.; Zharov, Vladimir P.

    2017-03-01

    Roughly 0.6 million people die each year from malaria due to lack of early diagnosis and well-timed treatment. Our previous study demonstrated great potential of in vivo photoacoustic (PA) flow cytometry (PAFC) for early diagnosis of deadly diseases with focus on cancer and thromboembolic complications. Here we demonstrate potential of advanced PAFC platforms using new laser, ultrasound transducer array and recording system to detect infected red blood cells (iRBCs) with malaria-associated pigment hemozoin which has a higher PA contrast than blood background. Mature parasites of human infecting species such as P. falciparum characteristically sequester mature iRBCs in the capillary bed and display synchrony in their reproductive cycle. To address this issue prior to clinical application, new PAFC platform was verified in a pre-clinical study using new animal models. Specifically, we used P. chabaudi (a rodent malaria species that mimics the characteristics of the most virulent human counterpart) to estimate the detection sensitivity with immature ring-stage parasites in peripheral blood, compared PA signals from the differing species, and examined the relationship between PA signal amplitudes and level of blood oxygenation. Based on previous successful trials on melanoma patients with melanin as an intrinsic PA marker, which has similar absorption as hemozoin, we believe that after additional malaria-related clinical trials, PAFC with a small 1064 nm laser and wearable a cost-effective, easy-to-use, watch-like, safe PA probe will provide malaria diagnosis in humans at parasitemia levels 10e4 -times lower than the current gold standard of diagnosis, the Giemsa-stained blood smear. It can reduce malaria-related mortality by well-timed treatment, especially in children in malaria-endemic countries.

  7. Ouabain enhancement of compound 48/80 induced histamine secretion from rat peritoneal mast cells: dependence on extracellular sodium

    DEFF Research Database (Denmark)

    Knudsen, T; Bertelsen, Niels Haldor; Johansen, Torben

    1992-01-01

    a measure of the Na(+)-K+ pump activity of the cells. Ouabain caused an immediate inhibition of the pump activity and a time-dependent increase in histamine secretion in the absence of extracellular calcium. No effect on the secretion was observed in the presence of calcium. The effect of ouabain......-free medium, the pump activity was inhibited and the enhancement by ouabain of the secretion of histamine was blocked. A less marked inhibition of the pump was found in a calcium-free medium containing magnesium. The inhibition exerted by magnesium was concentration-dependent (0-5 mM) as was the counteraction...... of sodium-calcium exchange caused by a decreased inward directed sodium gradient, the mechanism by which ouabain enhances the secretory response is likely to involve an increased binding of calcium to membrane binding sites....

  8. Ouabain, a cardiac glycoside, inhibits the Fanconi anemia/BRCA pathway activated by DNA interstrand cross-linking agents.

    Directory of Open Access Journals (Sweden)

    Dong Wha Jun

    Full Text Available Modulation of the DNA repair pathway is an emerging target for the development of anticancer drugs. DNA interstrand cross-links (ICLs, one of the most severe forms of DNA damage caused by anticancer drugs such as cisplatin and mitomycin C (MMC, activates the Fanconi anemia (FA/BRCA DNA repair pathway. Inhibition of the FA/BRCA pathway can enhance the cytotoxic effects of ICL-inducing anticancer drugs and can reduce anticancer drug resistance. To find FA/BRCA pathway inhibitory small molecules, we established a cell-based high-content screening method for quantitating the activation of the FA/BRCA pathway by measuring FANCD2 foci on DNA lesions and then applied our method to chemical screening. Using commercial LOPAC1280 chemical library screening, ouabain was identified as a competent FA/BRCA pathway inhibitory compound. Ouabain, a member of the cardiac glycoside family, binds to and inhibits Na(+/K(+-ATPase and has been used to treat heart disease for many years. We observed that ouabain, as well as other cardiac glycoside family members--digitoxin and digoxin--down-regulated FANCD2 and FANCI mRNA levels, reduced monoubiquitination of FANCD2, inhibited FANCD2 foci formation on DNA lesions, and abrogated cell cycle arrest induced by MMC treatment. These inhibitory activities of ouabain required p38 MAPK and were independent of cellular Ca(2+ ion increase or the drug uptake-inhibition effect of ouabain. Furthermore, we found that ouabain potentiated the cytotoxic effects of MMC in tumor cells. Taken together, we identified an additional effect of ouabain as a FA/BRCA pathway-inhibiting chemosensitization compound. The results of this study suggest that ouabain may serve as a chemosensitizer to ICL-inducing anticancer drugs.

  9. Dopamine-mediated inhibition of renal Na+/K+-ATPase in HK-2 cells is reduced by ouabain.

    Science.gov (United States)

    Zhang, Yu-Rong; Yuan, Zu-Yi

    2010-05-01

    1. Abnormal renal sodium handling is considered a major contributing factor in hypertension associated with chronic ouabain treatment. However, the molecular mechanisms involved in abnormal renal sodium handling have not been elucidated. Therefore, we investigated whether chronic ouabain treatment perturbs dopamine D(1) receptor function. 2. The expression and phosphorylation levels of the D(1) receptor in cells of the human proximal tubule cell line (HK-2) were determined using western blot analysis and reverse transcription polymerase chain reaction. The activity of the renal sodium/potassium pump (Na(+)/K(+)-ATPase) was measured using a colourimetric assay, and cyclic adenosine monophosphate accumulation was determined by performing a radioimmunoassay. 3. We showed that chronic ouabain treatment decreased the protein and mRNA expression levels of the D(1) receptor and increased the basal phosphorylation of the D(1) receptor in HK-2 cells. We also showed that in the presence of ouabain, HK-2 cells did not reveal the cyclic adenosine monophosphate accumulation and Na(+)/K(+)-ATPase inhibition induced by the D(1) receptor agonist fenoldopam. 4. We hypothesize that the ouabain-induced decrease in renal D(1) receptor function is responsible for the increase in renal sodium reabsorption, which eventually leads to ouabain-induced hypertension.

  10. Pharmacokinetics of tritiated ouabain, digoxin and digitoxin in guinea-pigs.

    Science.gov (United States)

    Proppe, D

    1975-01-01

    1. Elimination rates of tritiated ouabain, digoxin and digitoxin after single intravenous administrations were investigated in guinea-pigs, the total radioactivity in whole blood being traced for a period of up to 2 weeks. 2. In the initial rapid phase of elimination between 2 and 30 min following intravenous glycoside administration, the concentration decline of radioactivity in the blood was found to be identical for the three glycosides investigated, this part of the elimination curve displaying a hyperbolic shape. 3. During this early elimination phase, rapid metabolic degradation and excretion of digoxin had already taken place. The maximum concentration of radioactivity in the bile was reached 4 min following intravenous administration of 3H-digoxin. The positive inotropic response occurred in the cat heart-lung preparation 1.5 min after intravenous injection of a therapeutic dose of digoxin, indicating a quick occupation of binding sites in the tissues. 4. The biological half-lives of tritiated ouabain, digoxin and digitoxin averaged 11 h, 2.5 days and 4.1 days, respectively, as determined by the terminal exponential elimination phase, in guinea-pigs. This terminal phase was attained 6-12, 7-24, and 24-48 h after administration of ouabain, digoxin and digitoxin, respectively. 5. The findings reveal that in guinea-pig, as has been demonstrated in man, the elimination rates of the three glycosides increase according to their hydrophobic properties. 6. The biological half-lives of tritiated ouabain, digoxin and digitoxin obtained in the guinea-pig closely resemble those found in healthy man.

  11. Ouabain-induced perturbations in intracellular ionic homeostasis regulate death receptor-mediated apoptosis

    Science.gov (United States)

    Panayiotidis, Mihalis I.; Franco, Rodrigo; Bortner, Carl D.; Cidlowski, John A.

    2012-01-01

    Apoptosis is defined by specific morphological and biochemical characteristics including cell shrinkage (termed apoptotic volume decrease), a process that results from the regulation of ion channels and plasma membrane transporter activity. The Na+-K+-ATPase is the predominant pump that controls cell volume and plasma membrane potential in cells and alterations in its function have been suggested to be associated with apoptosis. We report here that the Na+-K+-ATPase inhibitor ouabain, potentiates apoptosis in the human lymphoma Jurkat cells exposed to Fas ligand (FasL) or Tumor necrosis factor--related apoptosis-inducing ligand (TRAIL) but not other apoptotic agents such as H2O2, thapsigargin or UV-C implicating a role for the Na+-K+-ATPase in death receptor-induced apoptosis. Interestingly, ouabain also potentiated perturbations in cell Ca2+ homeostasis only in conjunction with the apoptotic inducer FasL but not TRAIL. Ouabain did not affect alterations in the intracellular Ca2+ levels in response to H2O2, thapsigargin or UV-C. FasL-induced alterations in Ca2+ were not abolished in Ca2+-free medium but incubation of cells with BAPTA-AM inhibited both Ca2+ perturbations and the ouabain-induced potentiation of FasL-induced apoptosis. Our data suggest that the impairment of the Na+-K+-ATPase activity during apoptosis is linked to perturbations in cell Ca2+ homeostasis that modulate apoptosis induced by the activation of Fas by FasL. PMID:20422450

  12. The testicular form of hormone-sensitive lipase HSLtes confers rescue of male infertility in HSL-deficient mice.

    Science.gov (United States)

    Vallet-Erdtmann, Virginie; Tavernier, Geneviève; Contreras, Juan Antonio; Mairal, Aline; Rieu, Cécile; Touzalin, Anne-Marie; Holm, Cecilia; Jégou, Bernard; Langin, Dominique

    2004-10-01

    Inactivation of the hormone-sensitive lipase gene (HSL) confers male sterility with a major defect in spermatogenesis. Several forms of HSL are expressed in testis. HSLtes mRNA and protein are found in early and elongated spermatids, respectively. The other forms are expressed in diploid germ cells and interstitial cells of the testis. To determine whether the absence of the testis-specific form of HSL, HSLtes, was responsible for the infertility in HSL-null mice, we generated transgenic mice expressing HSLtes under the control of its own promoter. The transgenic animals were crossed with HSL-null mice to produce mice deficient in HSL in nongonadal tissues but expressing HSLtes in haploid germ cells. Cholesteryl ester hydrolase activity was almost completely blunted in HSL-deficient testis. Mice with one allele of the transgene showed an increase in enzymatic activity and a small elevation in the production of spermatozoa. The few fertile hemizygous male mice produced litters of very small to small size. The presence of the two alleles led to a doubling in cholesteryl ester hydrolase activity, which represented 25% of the wild type values associated with a qualitatively normal spermatogenesis and a partial restoration of sperm reserves. The fertility of these mice was totally restored with normal litter sizes. In line with the importance of the esterase activity, HSLtes transgene expression reversed the cholesteryl ester accumulation observed in HSL-null mice. Therefore, expression of HSLtes and cognate cholesteryl ester hydrolase activity leads to a rescue of the infertility observed in HSL-deficient male mice.

  13. Intracerebroventricular administration of ouabain, a Na/K-ATPase inhibitor, activates mTOR signal pathways and protein translation in the rat frontal cortex.

    Science.gov (United States)

    Kim, Se Hyun; Yu, Hyun-Sook; Park, Hong Geun; Ha, Kyooseob; Kim, Yong Sik; Shin, Soon Young; Ahn, Yong Min

    2013-08-01

    Intracerebroventricular (ICV) injection of ouabain, a specific Na/K-ATPase inhibitor, induces behavioral changes in rats in a putative animal model of mania. The binding of ouabain to Na/K-ATPase affects signaling molecules in vitro, including ERK1/2 and Akt, which promote protein translation. We have also reported that ERK1/2 and Akt in the brain are involved in the ouabain-induced hyperactivity of rats. In this study, rats were given an ICV injection of ouabain, and then their frontal cortices were examined to determine the effects of ouabain on the mTOR/p70S6K/S6 signaling pathway and protein translation, which are important in modifications of neural circuits and behavior. Rats showed ouabain-induced hyperactivity up to 8h following injection, and increased phosphorylation levels of mTOR, p70S6K, S6, eIF4B, and 4E-BP at 1, 2, 4, and 8h following ouabain injection. Immunohistochemical analyses revealed that increased p-S6 immunoreactivity in the cytoplasm of neurons by ouabain was evident in the prefrontal, cingulate, and orbital cortex. These findings suggested increased translation initiation in response to ouabain. The rate of protein synthesis was measured as the amount of [(3)H]-leucine incorporation in the cell-free extracts of frontal cortical tissues, and showed a significant increase at 8h after ouabain injection. These results suggest that ICV injection of ouabain induced activation of the protein translation initiation pathway regulated by ERK1/2 and Akt, and prolonged hyperactivity in rats. In conclusion, protein translation pathway could play an important role in ouabain-induced hyperactivity in a rodent model of mania.

  14. Ouabain rescues rat nephrogenesis during intrauterine growth restriction by regulating the complement and coagulation cascades and calcium signaling pathway.

    Science.gov (United States)

    Chen, L; Yue, J; Han, X; Li, J; Hu, Y

    2016-02-01

    Intrauterine growth restriction (IUGR) is associated with a reduction in the numbers of nephrons in neonates, which increases the risk of hypertension. Our previous study showed that ouabain protects the development of the embryonic kidney during IUGR. To explore this molecular mechanism, IUGR rats were induced by protein and calorie restriction throughout pregnancy, and ouabain was delivered using a mini osmotic pump. RNA sequencing technology was used to identify the differentially expressed genes (DEGs) of the embryonic kidneys. DEGs were submitted to the Database for Annotation and Visualization and Integrated Discovery, and gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted. Maternal malnutrition significantly reduced fetal weight, but ouabain treatment had no significant effect on body weight. A total of 322 (177 upregulated and 145 downregulated) DEGs were detected between control and the IUGR group. Meanwhile, 318 DEGs were found to be differentially expressed (180 increased and 138 decreased) between the IUGR group and the ouabain-treated group. KEGG pathway analysis indicated that maternal undernutrition mainly disrupts the complement and coagulation cascades and the calcium signaling pathway, which could be protected by ouabain treatment. Taken together, these two biological pathways may play an important role in nephrogenesis, indicating potential novel therapeutic targets against the unfavorable effects of IUGR.

  15. Structure-activity relationships for the hypertensinogenic activity of ouabain: role of the sugar and lactone ring.

    Science.gov (United States)

    Manunta, P; Hamilton, B P; Hamlyn, J M

    2001-02-01

    Elevated levels of an endogenous ouabain circulate in many patients with essential hypertension. However, in contrast to ouabain, digoxin does not induce hypertension. This study investigated the hypothesis that within a single cardiac glycoside, the structural elements that induce hypertension differ from those responsible for high potency as a sodium pump inhibitor. Normal male Sprague-Dawley rats received infusions of vehicle (VEH), rhamnose (RHA), ouabain (OUA), ouabagenin (OGN), dihydro-ouabain (DHO), iso-ouabain (ISO), and a lactone ring opened analog (ORO) at 30 microgram. kg(-1). 24 h(-1) for 5 weeks via subcutaneous osmotic pumps. Cuff pressures were taken weekly. At the end of the study, trunk blood was harvested, extracted by C18 column, and subjected to high-performance liquid chromatography. Fractions were analyzed for OUA, OGN, and DHO by immunoassay. In OUA-, OGN-, and DHO-infused rats, 1 main peak of immunoreactivity corresponding to the infused agent was found. No evidence of in vivo conversion to OUA or DHO was found for any analog except ORO. At 5 weeks, systolic blood pressures in VEH, RHA, OUA, OGN, DHO, ISO, and ORO were 132+/-2.5, 133+/-1.5, 159+/-2.6,* 154+/-4,* 167+/-4,* 171+/-2.2,* and 169+/-2.4* mm Hg, respectively (*Pmechanism of action independent of the sodium pump.

  16. Alternating Hemiplegia of Childhood mutations have a differential effect on Na(+),K(+)-ATPase activity and ouabain binding.

    Science.gov (United States)

    Weigand, Karl M; Messchaert, Muriël; Swarts, Herman G P; Russel, Frans G M; Koenderink, Jan B

    2014-07-01

    De novo mutations in ATP1A3, the gene encoding the α3-subunit of Na(+),K(+)-ATPase, are associated with the neurodevelopmental disorder Alternating Hemiplegia of Childhood (AHC). The aim of this study was to determine the functional consequences of six ATP1A3 mutations (S137Y, D220N, I274N, D801N, E815K, and G947R) associated with AHC. Wild type and mutant Na(+),K(+)-ATPases were expressed in Sf9 insect cells using the baculovirus expression system. Ouabain binding, ATPase activity, and phosphorylation were absent in mutants I274N, E815K and G947R. Mutants S137Y and D801N were able to bind ouabain, although these mutants lacked ATPase activity, phosphorylation, and the K(+)/ouabain antagonism indicative of modifications in the cation binding site. Mutant D220N showed similar ouabain binding, ATPase activity, and phosphorylation to wild type Na(+),K(+)-ATPase. Functional impairment of Na(+),K(+)-ATPase in mutants S137Y, I274N, D801N, E815K, and G947R might explain why patients having these mutations suffer from AHC. Moreover, mutant D801N is able to bind ouabain, whereas mutant E815K shows a complete loss of function, possibly explaining the different phenotypes for these mutations.

  17. Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes

    Directory of Open Access Journals (Sweden)

    Mabel B. Esteves

    2005-06-01

    Full Text Available Lymphocytes activated by mitogenic lectins display changes in transmembrane potential, an elevation in the cytoplasmic Ca2+ concentrations, proliferation and/or activation induced cell death. Low concentrations of ouabain (an inhibitor of Na+,K+-ATPase suppress mitogen-induced proliferation and increases cell death. To understand the mechanisms involved, a number of parameters were analyzed using fluorescent probes and flow cytometry. The addition of 100nM ouabain to cultures of peripheral blood lymphocytes activated with 5µg/ml phytohemagglutinin (PHA did not modify the increased expression of the Fas receptor or its ligand FasL induced by the mitogen. However, treatment with ouabain potentiated apoptosis induced by an anti-Fas agonist antibody. A synergy between ouabain and PHA was also observed with regard to plasma membrane depolarization. PHA per se did not induce dissipation of mitochondrial membrane potential but when cells were also exposed to ouabain a marked depolarization could be observed, and this was a late event. It is possible that the inhibitory effect of ouabain on activated peripheral blood lymphocytes involves the potentiation of some of the steps of the apoptotic process and reflects an exacerbation of the mechanism of activation-induced cell death.Quando linfócitos são ativados por lectinas mitogênicas apresentam mudanças do potencial de membrana, elevação das concentrações citoplasmáticas de cálcio, proliferação e/ou morte celular induzida por ativação (AICD. Concentrações baixas de ouabaína (um inibidor da Na,K-ATPase suprimem a proliferação induzida por mitógenos e aumentam a morte celular. Para entender os mecanismos envolvidos, uma série de parâmetros foram avaliados usando sondas fluorescentes e citometria de fluxo. A adição de 100nM de ouabaína para culturas de linfócitos de sangue periférico ativadas por fitohemaglutinina (PHA não modificou o aumento de expressão do receptor Fas ou de

  18. Regulation by L channels of Ca(2+)-evoked secretory responses in ouabain-treated chromaffin cells.

    Science.gov (United States)

    De Pascual, Ricardo; Colmena, Inés; Ruiz-Pascual, Lucía; Baraibar, Andrés Mateo; Egea, Javier; Gandía, Luis; García, Antonio G

    2016-10-01

    It is known that the sustained depolarisation of adrenal medullary bovine chromaffin cells (BCCs) with high K(+) concentrations produces an initial sharp catecholamine release that subsequently fades off in spite depolarisation persists. Here, we have recreated a sustained depolarisation condition of BCCs by treating them with the Na(+)/K(+) ATPase blocker ouabain; in doing so, we searched experimental conditions that permitted the development of a sustained long-term catecholamine release response that could be relevant during prolonged stress. BCCs were perifused with nominal 0Ca(2+) solution, and secretion responses were elicited by intermittent application of short 2Ca(2+) pulses (Krebs-HEPES containing 2 mM Ca(2+)). These pulses elicited a biphasic secretory pattern with an initial 30-min period with secretory responses of increasing amplitude and a second 30-min period with steady-state, non-inactivating responses. The initial phase was not due to gradual depolarisation neither to gradual increases of the cytosolic calcium transients ([Ca(2+)]c) elicited by 2Ca(2+) pulses in BBCs exposed to ouabain; both parameters increased soon after ouabain addition. Νifedipine blocked these responses, and FPL64176 potentiated them, suggesting that they were triggered by Ca(2+) entry through non-inactivating L-type calcium channels. This was corroborated by nifedipine-evoked blockade of the L-type Ca(2+) channel current and the [Ca(2+)]c transients elicited by 2Ca(2+) pulses. Furthermore, the plasmalemmal Na(+)/Ca(2+) exchanger (NCX) blocker SEA0400 caused a mild inhibition followed by a large rebound increase of the steady-state secretory responses. We conclude that these two phases of secretion are mostly contributed by Ca(2+) entry through L calcium channels, with a minor contribution of Ca(2+) entry through the reverse mode of the NCX.

  19. Potentiation of K+-evoked catecholamine release in the cat adrenal gland treated with ouabain.

    Science.gov (United States)

    Garcia, A. G.; Garcia-Lopez, E.; Horga, J. F.; Kirpekar, S. M.; Montiel, C.; Sanchez-Garcia, P.

    1981-01-01

    1 A vigorous catecholamine secretory response was evoked by small increments (2-10 mM) of the extracellular concentration of K+ ([K+])o) in cat adrenal glands treated with ouabain (10(-4) M), and perfused with Krebs-bicarbonate solution at room temperature. 2 The secretory response depends on [K+]o; increments of [K+]o as small as 2 mM for 2 min evoked a clear secretory response; at 10-17.7 mM K+, the maximal secretory response was observed. In normal glands, not treated with ouabain, no increase of the rate of catecholamine output was observed by raising [K+]o up to 17.7 mM for 2 min. 3 The K+ secretory response was time-dependent, requiring at least 1 min to be initiated; on continued exposure to 10 mM [K+]o, the enhanced response remained for at least 1 h. 4 In low [Na+]o, the K+-secretory response was unchanged. However, in 0-Ca2+, high-Mg2+ solutions, or in the presence of D600, an organic Ca2+ antagonist, it was abolished. 5 The K+-induced secretory response was not altered in the presence of tetrodoxin or tetraethylammonium. 6 It is concluded that ouabain potentiated the catecholamine secretory response to raised [K+]o by increasing the amount of Ca2+ available to the secretory machinery through (a) mobilization of an enhanced pool of membrane-bound Ca2+, (b) activation of membrane Ca2+ inward current; or (c) decrease of intracellular Ca2+ buffering systems. The activation by ouabain of a membrane Na+-Ca2+ exchange system is not involved in this K+-secretory response. It is suggested that the plasma membrane ATPase enzyme system, by changing the affinity of its Ca2+ binding sites, might control the availability of this cation to the secretory machinery and, therefore, modulate catecholamine secretion in the adrenal gland. PMID:7296168

  20. Ouabain mimics low temperature rescue of F508del-CFTR in cystic fibrosis epithelial cells

    Directory of Open Access Journals (Sweden)

    Donglei eZhang

    2012-10-01

    Full Text Available ABSTRACT Most cases of cystic fibrosis (CF are caused by the deletion of a single phenylalanine residue at position 508 of the cystic fibrosis transmembrane conductance regulator (CFTR. The mutant F508del-CFTR is retained in the endoplasmic reticulum and degraded, but can be induced by low temperature incubation (29°C to traffic to the plasma membrane where it functions as a chloride channel. Here we show that, cardiac glycosides, at nanomolar concentrations, can partially correct the trafficking of F508del-CFTR in human CF bronchial epithelial cells (CFBE41o- and in an F508del-CFTR mouse model. Comparison of the transcriptional profiles obtained with polarized CFBE41o- cells after treatment with ouabain and by low temperature has revealed a striking similarity between the two corrector treatments that is not shared with other correctors. In summary, our study shows a novel function of ouabain and its analogues in the regulation of F508del-CFTR trafficking and suggests that compounds that mimic this low temperature correction of trafficking will provide new avenues for the development of therapeutics for CF.

  1. Measurement of specific [3H]-ouabain binding to different types of human leucocytes

    DEFF Research Database (Denmark)

    Boon, Arnold; Oh, V M; Taylor, John E.

    1984-01-01

    -dependent, competitively antagonized by potassium, and facilitated by the presence of divalent cations. The equilibrium dissociation constants were 2.4 +/- 0.7 nmol/l (polymorphs) and 2.4 +/- 0.4 nmol/l (mononuclear cells) (NS). The values of maximal specific ouabain binding, measured by Scatchard analysis...... of concentration vs binding curves (Bmax), were 33.9 +/- 6.0 fmol/10(6) cells (polymorphs) and 59.3 +/- 11.6 fmol/10(6) cells (mononuclear cells) (P less than 0.02). The corresponding numbers of sites per cell were 20415 +/- 3616 and 35712 +/- 6986 respectively (P less than 0.02). When the numbers of binding sites...... were expressed per square micron of cell surface area the difference between the two cell types was proportionately greater (83 and 186 sites per micron 2 respectively). We conclude that the [3H]-ouabain binding sites on mononuclear and polymorphonuclear leucocytes are similar in nature, but different...

  2. Effect of ouabain, digoxin and digitoxigenin on potassium uptake and histamine release from rat peritoneal mast cells

    DEFF Research Database (Denmark)

    Knudsen, T; Ferjan, I; Johansen, Torben

    1993-01-01

    Rat peritoneal mast cells were used to study the effects of digitalis glycosides on potassium uptake and histamine release induced by compound 48/80, substance P and egg-albumin (immunological release). In the absence of calcium all glycosides inhibited potassium uptake. Ouabain and digoxin...

  3. EFFECTS OF OUABAIN AND DIGOXIN ON THE GENE EXPRESSION OF SODIUM PUMP α-SUBUNIT ISOFORM IN AORTIC SMOOTH MUSCLE OF RATS

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective To compare the effects of ouabain and digoxin on both the systolic blood pressure and sodium pump α-subunit isoforms gene expression in the aortic smooth muscle of rats. Methods Normal SpragueDawley rats were injected with ouabain (20μg·kg-1 ·d-1 ,i. p),digoxin (32 μg·kg-1 ·d-1,i. p)and normal saline once a day, respectively, and indirect systolic blood pressure was recorded once a week. Six weeks later,all the rats were killed and sodium pump α1-,α2-,and α3-subunit mRNA levels were detected in the aortic smooth muscle with reverse transcription polymerase chain reaction(RT-PCR) method. Results The systolic blood pressure of rats infused with ouabain increased significantly at the end of week 6 [132. 6± 9. 0 mmHg (1 mmHg = 0. 133 kPa)vs 115. 7±8.2mmHg, P <0. 01] ,while no difference of blood pressure was found between digoxin group and NS group (P>0.05).The expression of sodium pump α-subunit isoforms in aortic smooth muscle was regulated by either ouabain or digoxin:both ouabain and digoxin increased α1- and α3-subunit expression, α2-subunit decreased in digoxin group but unchanged in ouabain group. Conclusion These results suggest that both ouabain and digoxin could regulate sodium pump α-subunit isoform expression, which might be related to the physiological roles of endogenous ouabain and might be responsible for the difference between the pharmacological and toxicological effects of ouabain and digoxin,including their effects on blood pressure.

  4. Ouabain-binding and 86rubidium-uptake in lymphocytes of normal and borderline hypertensive subjects

    DEFF Research Database (Denmark)

    Nielsen, J R; Pedersen, K E; Johansen, Torben;

    1983-01-01

    activity were studied in lymphocytes of nine borderline hypertensives (27 (20-36) years) and nine controls (28 (20-36) years). Maximum 3H-ouabain binding and 86Rb-uptake were taken as measures of the number of pump sites and cation pump activity, respectively. The median number of sodium/potassium pump...... sites was 49.6 X 10(3) molecules/cell in the BH group compared to 32.4 X 10(3) in the control group (P less than 0.01). Median 90 min 86Rb-uptakes were 54.0 pmol/10(6) cells in BH subjects and 39.4 in controls (P less than 0.10). The increased number of sodium/potassium pump sites and the tendency...... to increased cation pump activity in lymphocytes of BH subjects in vitro may be interpreted as an adaptive change possibly induced by a circulating natriuretic substance....

  5. Glucocorticoid receptor haplotypes conferring increased sensitivity (Bcll and N363S) are associated with faster progression of multiple sclerosis.

    NARCIS (Netherlands)

    Melief, J.; Koper, J.W.; Endert, E.; Moller, H.; Hamann, J.; Uitdehaag, B.M.; Huitinga, I.

    2016-01-01

    As high cortisol levels are implicated in suppressed disease activity of multiple sclerosis (MS), glucocorticoid receptor (GR) polymorphisms that affect glucocorticoid (GC) sensitivity may impact on this by changing local immunomodulation or regulation of the hypothalamus–pituitary–adrenal (HPA)-axi

  6. Intracerebroventricular administration of ouabain, a Na/K-ATPase inhibitor, activates tyrosine hydroxylase through extracellular signal-regulated kinase in rat striatum.

    Science.gov (United States)

    Yu, Hyun Sook; Kim, Se Hyun; Park, Hong Geun; Kim, Yong Sik; Ahn, Yong Min

    2011-11-01

    Alteration in dopamine neurotransmission has been reported to be involved in the mania of bipolar disorder. Tyrosine hydroxylase (TH) is the rate-limiting enzyme that is crucial for dopamine biosynthesis, and its activity is tightly regulated by phosphorylation at multiple N-terminal serine residues. Previously, we have reported that intracerebroventricular (ICV) injection of ouabain, a selective Na/K-ATPase inhibitor, induces hyperactivity in rats that mimics manic symptoms related to the activation of extracellular signal-regulated protein kinase1/2 (ERK1/2), which plays crucial roles in the modulation of TH phosphorylation. In this study, we investigated the effects of ICV injection of ouabain on TH phosphorylation in rat striatum and the involvement of ERK1/2 in ouabain-induced TH activation. ICV ouabain induced an acute dose-dependent increase in locomotor activity and in TH phosphorylation in rat striatum. TH phosphorylation at Ser19 was significantly increased with 100, 500, and 1000μM ouabain, and phosphorylation at Ser31 and Ser40 was significantly increased with 500 and 1000μM. We also found that ICV pretreatment with U0126, a specific MEK1/2 inhibitor, attenuated the 1000μM ouabain-induced increase in TH phosphorylation at Ser19, Ser31, and Ser40, as well as the hyperactivity of rats. Moreover, the increased phosphorylation of TH (Ser19, Ser31, and Ser40) was maintained until 8h after single administration ouabain was accompanied by increased phosphorylation of ERK1/2 (Thr202/Tyr204) and p90RSK (Thr359/Ser363). These findings imply that TH activation of the ERK1/2 signal pathway could play an important role in ouabain-induced hyperactivity of rats, a mania model.

  7. Curcumin confers protection to irradiated THP-1 cells while its nanoformulation sensitizes these cells via apoptosis induction.

    Science.gov (United States)

    Soltani, Behrooz; Ghaemi, Nasser; Sadeghizadeh, Majid; Najafi, Farhood

    2016-12-01

    Protection against ionizing radiation (IR) and sensitization of cancer cells to IR are apparently contrasting phenomena. However, curcumin takes on these contrasting roles leading to either protection or enhanced apoptosis in different irradiated cells. Here we studied whether pretreatment with free curcumin or a novel dendrosomal nanoformulation of curcumin (DNC) could exert protective/sensitizing effects on irradiated THP-1 leukemia cells. We employed assays including MTT viability, clonogenic survival, DNA fragmentation, PI/Annexin V flow cytometry, antioxidant system (ROS, TBARS for lipid peroxidation, 8-OHdG and γH2AX for DNA damage, glutathione, CAT and GPx activity, enzymes gene expression), ELISA (NF-κB and Nrf2 binding, TNF-α release), caspase assay, siRNA silencing of caspase-3, and western blotting to illustrate the observed protective role of curcumin in comparison with the opposite sensitizing role of its nanoformulation at a similar 10 μM concentration. The in vivo relevance of this concentration was determined via intraperitoneal administration in mice. Curcumin significantly enhanced the antioxidant defense, while DNC induced apoptosis and reduced viability as well as survival of irradiated THP-1 cells. Nrf2 binding showed an early rise and fall in DNC-treated cells, despite a gradual increase in curcumin-treated cells. We also demonstrated that DNC induced apoptosis in THP-1 cells via caspase-3 activation; whereas in combination with radiation, DNC alternatively employed a caspase-independent apoptosis pathway involving cytochrome c release from mitochondria.

  8. Investigation of stress-induced birefringence of tissue determined with polarisation sensitive optical coherence tomography (Conference Presentation)

    Science.gov (United States)

    Karnowski, Karol; Li, Qingyun; Villiger, Martin; Sampson, David D.

    2017-02-01

    Polarisation sensitive optical coherence tomography (PS-OCT) offers additional intrinsic contrast to probe differences between healthy tissue and cancer that are often barely visible due to limited scattering contrast in an OCT image. PS-OCT reconstructs tissue birefringence from phase-sensitive measurements of orthogonal polarisation components of backscattering. In material science, polarisation has been used to study stress distribution, including the birefringence induced by stress in an otherwise isotropic material. Similar effects in biological tissues have not been well studied yet; however, may have application to tissues subjected to stress, e.g., tendons, muscles, lens, cornea or airway smooth muscle (ASM). The objective of this work is to explore stress-induced birefringence in tissue. We employ an advanced swept source-based PS-OCT system capable of measurement of tissue local polarisation properties. The sample in both cases is illuminated with orthogonal, passively depth-encoded polarisation states. Light returning from the tissue is detected via a polarisation-diversity detection module and a Mueller formalism is used to reconstruct polarisation properties (including retardation, diattenuation, and depolarisation) of the tissue. In this study, we demonstrate the measurement of stress-induced birefringence in phantoms and in soft tissues with polarisation sensitive optical coherence tomography.

  9. Lithium and valproate modulate antioxidant enzymes and prevent ouabain-induced oxidative damage in an animal model of mania.

    Science.gov (United States)

    Jornada, Luciano K; Valvassori, Samira S; Steckert, Amanda V; Moretti, Morgana; Mina, Francielle; Ferreira, Camila L; Arent, Camila O; Dal-Pizzol, Felipe; Quevedo, João

    2011-02-01

    In this study, we assessed the oxidative stress parameters in rats submitted to an animal model of mania induced by ouabain (OUA), which included the use of lithium (Li) and valproate (VPA). Li and VPA treatment reversed and prevented the OUA-induced damage in these structures, however, this effect varies depending on the brain region and treatment regimen. Moreover, the activity of the antioxidant enzymes, namely, superoxide dismutase (SOD) and catalase (CAT) was found to be increased and decreased, respectively, in the brain of OUA-administered rats. Li and VPA modulated SOD and CAT activities in OUA-subjected rats in both experimental models. Our results support the notion that Li and VPA exert antioxidant-like properties in the brain of rats submitted to animal model of mania induced by ouabain.

  10. Erythrocytes may contain a ouabain-insensitive K+-ATPase which plays a role in internal K+ balance

    Directory of Open Access Journals (Sweden)

    Seguro L.F.B.C.

    2003-01-01

    Full Text Available Erythrocytes are useful in evaluating K+ transport pathways involved in internal K+ balance. Several forms of H+,K+-ATPase have been described in nephron segments active in K+ transport. Furthermore, the activity of a ouabain-insensitive isoform of H+,K+-ATPase expressed in collecting duct cells may be modulated by acid-base status. Various assays were performed to determine if a ouabain-insensitive K+-ATPase is present in rat erythrocytes and, if so, whether it plays a role in internal K+ balance. Kinetic studies demonstrated that maximal stimulation of enzyme activity was achieved with 2.5 mM K+ at pH 7.4. Subsequent experiments were performed on erythrocyte membranes collected from animals submitted to varying degrees of K+ homeostasis: control rats, K+-depleted rats, K+-loaded rats, and rats rendered hyperkalemic due to acute renal failure. As observed in the collecting duct cell studies, there was a significant decrease in the activity of ouabain-insensitive K+-ATPase in the erythrocytes of both K+-loaded and metabolically alkalotic K+-depleted rats. However, this enzyme activity in erythrocyte membranes of rats with metabolic acidosis-related hyperkalemia was similar to that of control animals. This finding may be interpreted as resulting from two potentially modulating factors: the stimulating effect that metabolic acidosis has on K+-ATPase and the counteracting effect that hyperkalemia and uremia have on metabolic acidosis. In summary, we present evidence of a ouabain-insensitive K+-ATPase in erythrocytes, whose activity is modulated by acid-base status and K+ levels.

  11. Wavenumber selection based analysis in Raman spectroscopy improves skin cancer diagnostic specificity at high sensitivity levels (Conference Presentation)

    Science.gov (United States)

    Zhao, Jianhua; Zeng, Haishan; Kalia, Sunil; Lui, Harvey

    2017-02-01

    Background: Raman spectroscopy is a non-invasive optical technique which can measure molecular vibrational modes within tissue. A large-scale clinical study (n = 518) has demonstrated that real-time Raman spectroscopy could distinguish malignant from benign skin lesions with good diagnostic accuracy; this was validated by a follow-up independent study (n = 127). Objective: Most of the previous diagnostic algorithms have typically been based on analyzing the full band of the Raman spectra, either in the fingerprint or high wavenumber regions. Our objective in this presentation is to explore wavenumber selection based analysis in Raman spectroscopy for skin cancer diagnosis. Methods: A wavenumber selection algorithm was implemented using variably-sized wavenumber windows, which were determined by the correlation coefficient between wavenumbers. Wavenumber windows were chosen based on accumulated frequency from leave-one-out cross-validated stepwise regression or least and shrinkage selection operator (LASSO). The diagnostic algorithms were then generated from the selected wavenumber windows using multivariate statistical analyses, including principal component and general discriminant analysis (PC-GDA) and partial least squares (PLS). A total cohort of 645 confirmed lesions from 573 patients encompassing skin cancers, precancers and benign skin lesions were included. Lesion measurements were divided into training cohort (n = 518) and testing cohort (n = 127) according to the measurement time. Result: The area under the receiver operating characteristic curve (ROC) improved from 0.861-0.891 to 0.891-0.911 and the diagnostic specificity for sensitivity levels of 0.99-0.90 increased respectively from 0.17-0.65 to 0.20-0.75 by selecting specific wavenumber windows for analysis. Conclusion: Wavenumber selection based analysis in Raman spectroscopy improves skin cancer diagnostic specificity at high sensitivity levels.

  12. Ouabain Modulates the Lipid Composition of Hippocampal Plasma Membranes from Rats with LPS-induced Neuroinflammation.

    Science.gov (United States)

    Garcia, Israel José Pereira; Kinoshita, Paula Fernanda; Scavone, Cristoforo; Mignaco, Julio Alberto; Barbosa, Leandro Augusto de Oliveira; Santos, Hérica de Lima

    2015-12-01

    The effects of ouabain (OUA) and lipopolysaccharide (LPS) in vivo on hippocampal membranes (RHM) of Wistar male rats aged 3 months were analyzed. After intraperitoneal (i.p.) injection of OUA only, LPS only, OUA plus LPS, or saline, the content of proteins, phospholipids, cholesterol and gangliosides from RHM was analyzed. The total protein and cholesterol contents of RHM were not significantly affected by OUA or LPS for the experimentally paired groups. In contrast, total phospholipids and gangliosides were strongly modulated by either OUA or LPS treatments. LPS reduced the total phospholipids (roughly 23 %) and increased the total gangliosides (approximately 40 %). OUA alone increased the total phospholipids (around 23 %) and also the total gangliosides (nearly 34 %). OUA pretreatment compensated the LPS-induced changes, preserving the total phospholipids and gangliosides around the same levels of the control. Thus, an acute treatment with OUA not only modulated the composition of hippocampal membranes from 3-month-old rats, but also was apparently able to counteract membrane alterations resulting from LPS-induced neuroinflammation. This study demonstrates for the first time that the OUA capacity modulates the lipid composition of hippocampal plasma membranes from rats with LPS-induced neuroinflammation.

  13. The Constitutively Active V2 Receptor Mutants Conferring NSIAD Are Weakly Sensitive to Agonist and Antagonist Regulation

    Science.gov (United States)

    Perkovska, Sanja; Adra-Delenne, Anne-Laure; Mendre, Christiane; Ranchin, Bruno; Bricca, Giamperro; Geelen, Ghislaine; Mouillac, Bernard; Durroux, Thierry; Morin, Denis

    2009-01-01

    Patients having the nephrogenic syndrome of inappropriate antidiuresis present either the R137C or R137L V2 mutated receptor. While the clinical features have been characterized, the molecular mechanisms of functioning of these two mutants remain elusive. In the present study, we compare the pharmacological properties of R137C and R137L mutants with the wild-type and the V2 D136A receptor, the latter being reported as a highly constitutively active receptor. We have performed binding studies, second messenger measurements and BRET experiments in order to evaluate the affinities of the ligands, their agonist and antagonist properties and the ability of the receptors to recruit β-arrestins, respectively. The R137C and R137L receptors exhibit small constitutive activities regarding the Gs protein activation. In addition, these two mutants induce a constitutive β-arrestin recruitment. Of interest, they also exhibit weak sensitivities to agonist and to inverse agonist in term of Gs protein coupling and β-arrestin recruitment. The small constitutive activities of the mutants and the weak regulation of their functioning by agonist suggest a poor ability of the antidiuretic function to be adapted to the external stimuli, giving to the environmental factors an importance which can explain some of the phenotypic variability in patients having NSIAD. PMID:20027297

  14. Assessment of vascularization and myelination following peripheral nerve repair using angiographic and polarization sensitive optical coherence tomography (Conference Presentation)

    Science.gov (United States)

    Nam, Ahhyun S.; Chico-Calero, Isabel; Easow, Jeena M.; Villiger, Martin; Welt, Jonathan; Winograd, Jonathan M.; Randolph, Mark A.; Redmond, Robert W.; Vakoc, Benjamin J.

    2017-02-01

    A severe traumatic injury to a peripheral nerve often requires surgical graft repair. However, functional recovery after these surgical repairs is often unsatisfactory. To improve interventional procedures, it is important to understand the regeneration of the nerve grafts. The rodent sciatic nerve is commonly used to investigate these parameters. However, the ability to longitudinally assess the reinnervation of injured nerves are limited, and to our knowledge, no methods currently exist to investigate the timing of the revascularization in functional recovery. In this work, we describe the development and use of angiographic and polarization-sensitive (PS) optical coherence tomography (OCT) to visualize the vascularization, demyelination and remyelination of peripheral nerve healing after crush and transection injuries, and across a variety of graft repair methods. A microscope was customized to provide 3.6 cm fields of view along the nerve axis with a capability to track the nerve height to maintain the nerve within the focal plane. Motion artifact rejection was implemented in the angiography algorithm to reduce degradation by bulk respiratory motion in the hindlimb site. Vectorial birefringence imaging methods were developed to significantly enhance the accuracy of myelination measurements and to discriminate birefringent contributions from the myelin and epineurium. These results demonstrate that the OCT platform has the potential to reveal new insights in preclinical studies and may ultimately provide a means for clinical intra-surgical assessment of peripheral nerve function.

  15. Using polarization-sensitive optical coherence tomography to identify tumor stromal fibrosis and increase tumor biopsy yield (Conference Presentation)

    Science.gov (United States)

    Hariri, Lida P.; Adams, David C.; Miller, Alyssa J.; Mino-Kenudson, Mari; Suter, Melissa J.

    2016-03-01

    Tissue biopsy is the principal method used to diagnose tumors in a variety of organ systems. It is essential to maximize tumor yield in biopsy specimens for both clinical diagnostic and research purposes. This is particularly important in tumors where additional tissue is needed for molecular analysis to identify patients who would benefit from mutation-specific targeted therapy, such as in lung carcinomas. Inadvertent sampling of fibrotic stroma within tumor nodules contaminates biopsies, decreases tumor yield, and can impede diagnosis. The ability to assess tumor composition and guide biopsy site selection in real time is likely to improve diagnostic yield. Polarization sensitive OCT (PS-OCT) measures birefringence in organized tissues, such as collagen, and could be used to distinguish tumor from fibrosis. In this study, PS-OCT was obtained in 65 lung nodule samples from surgical resection specimens containing varying ratios of tumor and fibrosis. PS-OCT was obtained with either a custom-built helical scanning catheter (0.8 or 1.6mm in diameter) or a dual-axis bench top scanner. Strong birefringence was observed in nodules containing dense fibrosis, with no birefringence in adjacent regions of tumor. Tumors admixed with early, loosely-organized collagen demonstrated mild-to-moderate birefringence, and tumors with little collagen content showed little to no birefringent signal. PS-OCT provides significant insights into tumor nodule composition, and has potential to differentiate tumor from stromal fibrosis during biopsy site selection to increase diagnostic tumor yield.

  16. Expression of human hormone-sensitive lipase (HSL) in postmeiotic germ cells confers normal fertility to HSL-deficient mice.

    Science.gov (United States)

    Wang, Shu Pei; Chung, Shari; Soni, Krishnakant; Bourdages, Hugo; Hermo, Louis; Trasler, Jacquetta; Mitchell, Grant A

    2004-12-01

    Hormone-sensitive lipase (HSL, Lipe, E.C.3.1.1.3) is a multifunctional fatty acyl esterase that is essential for male fertility and spermatogenesis and that also plays important roles in the function of adipocytes, pancreatic beta-cells, and adrenal cortical cells. Gene-targeted HSL-deficient (HSL-/-) male mice are infertile, have a 2-fold reduction in testicular mass, a 2-fold elevation of the ratio of esterified to free cholesterol in testis, and unique morphological abnormalities in round and elongating spermatids. Postmeiotic germ cells in the testis express a specific HSL isoform. We created transgenic mice expressing a normal human testicular HSL cDNA from the mouse protamine-1 promoter, which mediates expression specifically in postmeiotic germ cells. Testicular cholesteryl esterase activity was undetectable in HSL-/- mice, but in HSL-/- males expressing the testicular transgene, activity was 2-fold greater than normal. HSL transgene mRNA became detectable in testes between 19 and 25 days of age, coinciding with the first wave of postmeiotic transcription in round spermatids. In contrast to nontransgenic HSL-/- mice, HSL-/- males expressing the testicular transgene were normal with respect to fertility, testicular mass, testicular esterified/free cholesterol ratio, and testicular histology. Their cauda epididymides contained abundant, normal-appearing spermatozoa. We conclude that human testicular HSL is functional in mouse testis and that the mechanism of infertility in HSL-deficient males is cell autonomous and resides in postmeiotic germ cells, because HSL expression in these cells is in itself sufficient to restore normal fertility.

  17. Effects of Ouabain on Proliferation of Human Endothelial Cells Correlate with Na+,K+-ATPase Activity and Intracellular Ratio of Na+ and K.

    Science.gov (United States)

    Tverskoi, A M; Sidorenko, S V; Klimanova, E A; Akimova, O A; Smolyaninova, L V; Lopina, O D; Orlov, S N

    2016-08-01

    Side-by-side with inhibition of the Na+,K+-ATPase ouabain and other cardiotonic steroids (CTS) can affect cell functions by mechanisms other than regulation of the intracellular Na+ and K+ ratio ([Na+]i/[K+]i). Thus, we compared the dose- and time-dependences of the effect of ouabain on intracellular [Na+]i/[K+]i ratio, Na+,K+-ATPase activity, and proliferation of human umbilical vein endothelial cells (HUVEC). Treatment of the cells with 1-3 nM ouabain for 24-72 h decreased the [Na+]i/[K+]i ratio and increased cell proliferation by 20-50%. We discovered that the same ouabain concentrations increased Na+,K+-ATPase activity by 25-30%, as measured by the rate of (86)Rb(+) influx. Higher ouabain concentrations inhibited Na+,K+-ATPase, increased [Na+]i/[K+]i ratio, suppressed cell growth, and caused cell death. When cells were treated with low ouabain concentrations for 48 or 72 h, a negative correlation between [Na+]i/[K+]i ratio and cell growth activation was observed. In cells treated with high ouabain concentrations for 24 h, the [Na+]i/[K+]i ratio correlated positively with proliferation inhibition. These data demonstrate that inhibition of HUVEC proliferation at high CTS concentrations correlates with dissipation of the Na+ and K+ concentration gradients, whereas cell growth stimulation by low CTS doses results from activation of Na+,K+-ATPase and decrease in the [Na+]i/[K+]i ratio.

  18. msbB deletion confers acute sensitivity to CO2 in Salmonella enterica serovar Typhimurium that can be suppressed by a loss-of-function mutation in zwf

    Directory of Open Access Journals (Sweden)

    Troy Kimberly

    2009-08-01

    Full Text Available Abstract Background Pathogens tolerate stress conditions that include low pH, oxidative stress, high salt and high temperature in order to survive inside and outside their hosts. Lipopolysaccharide (LPS, which forms the outer-leaflet of the outer membrane in Gram-negative bacteria, acts as a permeability barrier. The lipid A moiety of LPS anchors it to the outer membrane bilayer. The MsbB enzyme myristoylates the lipid A precursor and loss of this enzyme, in Salmonella, is correlated with reduced virulence and severe growth defects that can both be compensated with extragenic suppressor mutations. Results We report here that msbB (or msbB somA Salmonella are highly sensitive to physiological CO2 (5%, resulting in a 3-log reduction in plating efficiency. Under these conditions, msbB Salmonella form long filaments, bulge and lyse. These bacteria are also sensitive to acidic pH and high osmolarity. Although CO2 acidifies LB broth media, buffering LB to pH 7.5 did not restore growth of msbB mutants in CO2, indicating that the CO2-induced growth defects are not due to the effect of CO2 on the pH of the media. A transposon insertion in the glucose metabolism gene zwf compensates for the CO2 sensitivity of msbB Salmonella. The msbB zwf mutants grow on agar, or in broth, in the presence of 5% CO2. In addition, msbB zwf strains show improved growth in low pH or high osmolarity media compared to the single msbB mutant. Conclusion These results demonstrate that msbB confers acute sensitivity to CO2, acidic pH, and high osmolarity. Disruption of zwf in msbB mutants restores growth in 5% CO2 and results in improved growth in acidic media or in media with high osmolarity. These results add to a growing list of phenotypes caused by msbB and mutations that suppress specific growth defects.

  19. A Paleolithic diet confers higher insulin sensitivity, lower C-reactive protein and lower blood pressure than a cereal-based diet in domestic pigs.

    Science.gov (United States)

    Jönsson, Tommy; Ahrén, Bo; Pacini, Giovanni; Sundler, Frank; Wierup, Nils; Steen, Stig; Sjöberg, Trygve; Ugander, Martin; Frostegård, Johan; Göransson, Leif; Lindeberg, Staffan

    2006-11-02

    from the Cereal group. This study in domestic pigs suggests that a Paleolithic diet conferred higher insulin sensitivity, lower C-reactive protein and lower blood pressure when compared to a cereal based diet.

  20. A Paleolithic diet confers higher insulin sensitivity, lower C-reactive protein and lower blood pressure than a cereal-based diet in domestic pigs

    Directory of Open Access Journals (Sweden)

    Ugander Martin

    2006-11-01

    between the groups, but leukocytes were clearly more frequent in sampled pancreas from the Cereal group. Conclusion This study in domestic pigs suggests that a Paleolithic diet conferred higher insulin sensitivity, lower C-reactive protein and lower blood pressure when compared to a cereal based diet.

  1. Voltage-clamp studies of transient inward current and mechanical oscillations induced by ouabain in ferret papillary muscle.

    Science.gov (United States)

    Karagueuzian, H S; Katzung, B G

    1982-06-01

    1. We studied the effects of a toxic concentration of ouabain on transmembrane electrical activity and on mechanical behaviour of right ventricular papillary muscles from ferrets in a single sucrose-gap using current clamp and voltage clamp.2. Ouabain (1.4-1.8 muM) induced oscillatory after-potentials and after-concentrations in current-clamp experiments. Voltage clamp showed that the oscillatory after-potential was caused by a transient inward current, similar to that in Purkinje fibres.3. The transient current had a sigmoidal dependence on the preceding (activating) voltage step V1, with a treshold around -13 mV and a plateau between +10 and 20 mV. There was a decline in current amplitude for more positive clamps. When activated by a fixed V1 voltage step, and measured at different repolarization levels V2, the transient current manifested an inverse dependence on V2 between -50 and -10 mV. No outward transient current could be detected. Total replacement of Na in the bathing medium by Tris or by sucrose abolished the transient current.4. Ouabain caused an increase of phasic (twitch) tension responses to voltage steps at all potentials without shifting the curve relating these variables on the voltage axis. The drug evoked an even greater increase in the tonic tension responses.5. After prolonged exposure, oscillatory mechanical responses were frequently recorded during positive voltage steps. Unlike the after-contraction, these mechanical fluctuations were not consistently damped and were not accompanied by detectable synchronous current fluctuations. Catecholamines and dibutyryl cyclic AMP markedly reduced the amplitude of the tonic contraction and the mechanical oscillations but increased their frequency. Caffeine had no effect on the tonic contraction amplitude but abolished the fluctuations.6. These results support the proposal that Ca is transiently released from the overloaded sarcoplasmic reticulum in ouabain-intoxicated muscle and may evoke oscillatory

  2. The BRAF{sup T1799A} mutation confers sensitivity of thyroid cancer cells to the BRAF{sup V600E} inhibitor PLX4032 (RG7204)

    Energy Technology Data Exchange (ETDEWEB)

    Xing, Joanna [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States); Liu, Ruixin; Xing, Mingzhao [Laboratory for Cellular and Molecular Thyroid Research, Division of Endocrinology and Metabolism, The Johns Hopkins University School of Medicine, Baltimore, MD 21287 (United States); Trink, Barry, E-mail: btrink@jhmi.edu [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States)

    2011-01-28

    Research highlights: {yields} Exciting therapeutic potential has been recently reported for the BRAF{sup V600E} inhibitor PLX4032 in melanoma. {yields} We tested the effects of PLX4032 on the growth of thyroid cancer cells which often harbor the BRAF{sup V600E} mutation. {yields} We observed a potent BRAF{sup V600E}-dependent inhibition of thyroid cancer cells by PLX4032. {yields} We thus demonstrated an important therapeutic potential of PLX4032 for thyroid cancer. -- Abstract: Aberrant signaling of the Ras-Raf-MEK-ERK (MAP kinase) pathway driven by the mutant kinase BRAF{sup V600E}, as a result of the BRAF{sup T1799A} mutation, plays a fundamental role in thyroid tumorigenesis. This study investigated the therapeutic potential of a BRAF{sup V600E}-selective inhibitor, PLX4032 (RG7204), for thyroid cancer by examining its effects on the MAP kinase signaling and proliferation of 10 thyroid cancer cell lines with wild-type BRAF or BRAF{sup T1799A} mutation. We found that PLX4032 could effectively inhibit the MAP kinase signaling, as reflected by the suppression of ERK phosphorylation, in cells harboring the BRAF{sup T1799A} mutation. PLX4032 also showed a potent and BRAF mutation-selective inhibition of cell proliferation in a concentration-dependent manner. PLX4032 displayed low IC{sub 50} values (0.115-1.156 {mu}M) in BRAF{sup V600E} mutant cells, in contrast with wild-type BRAF cells that showed resistance to the inhibitor with high IC{sub 50} values (56.674-1349.788 {mu}M). Interestingly, cells with Ras mutations were also sensitive to PLX4032, albeit moderately. Thus, this study has confirmed that the BRAF{sup T1799A} mutation confers cancer cells sensitivity to PLX4032 and demonstrated its specific potential as an effective and BRAF{sup T1799A} mutation-selective therapeutic agent for thyroid cancer.

  3. Membrane events and ionic processes involved in dopamine release from tuberoinfundibular neurons. I. Effect of the inhibition of the Na+,K+-adenosine triphosphatase pump by ouabain

    Energy Technology Data Exchange (ETDEWEB)

    Taglialatela, M.; Amoroso, S.; Kaparos, G.; Maurano, F.; Di Renzo, G.F.; Annunziato, L.

    1988-08-01

    In the present study we investigated the membrane events and the ionic processes which mediate the stimulatory effect of ouabain on the release of endogenous dopamine (DA) and previously taken-up (3H)DA release from rat hypothalamic tuberoinfundibular dopaminergic (TIDA) neurons. Ouabain (0.1-1 mM) dose-dependently stimulated endogenous DA and newly taken-up (3H)DA release. This effect was counteracted partially by nomifensine (10 microM). Removal of Ca++ ions from the extracellular space in the presence of the Ca++-chelator ethylene glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic acid prevented completely ouabain-elicited (3H)DA release. Lanthanum (1 mM) and cobalt (2 mM), two inorganic Ca++-entry blockers, were able to inhibit this stimulatory effect, whereas verapamil (10 microM) and nitrendipine (50 microM), two organic antagonists of the voltage-operated channel for Ca++ ions, failed to affect ouabain-induced (3H)DA release. By contrast, adriamycin (100-300 microM), a putative inhibitor of cardiac Na+-Ca++ antiporter, dose-dependently prevented ouabain-induced (3H)DA release from TIDA neurons. Finally, tetrodotoxin reduced digitalis-stimulated (3H)DA release. In conclusion, these results seem to be compatible with the idea that the inhibition of Na+,K+-adenosine triphosphatase by ouabain stimulates the release of (3H)DA from a central neuronal system like the TIDA tract and that this effect is critically dependent on the entrance of Ca++ ions into the nerve terminals of these neurons. In addition the Na+-Ca++ exchange antiporter appears to be the membrane system which transports Ca++ ions into the neuronal cytoplasm during Na+,K+-adenosine triphosphatase inhibition. The enhanced intracellular Ca++ availability triggers DA release which could occur partially through a carrier-dependent process.

  4. Membrane events and ionic processes involved in dopamine release from tuberoinfundibular neurons. I. Effect of the inhibition of the Na+,K+-adenosine triphosphatase pump by ouabain.

    Science.gov (United States)

    Taglialatela, M; Amoroso, S; Kaparos, G; Maurano, F; Di Renzo, G F; Annunziato, L

    1988-08-01

    In the present study we investigated the membrane events and the ionic processes which mediate the stimulatory effect of ouabain on the release of endogenous dopamine (DA) and "previously taken-up" [3H]DA release from rat hypothalamic tuberoinfundibular dopaminergic (TIDA) neurons. Ouabain (0.1-1 mM) dose-dependently stimulated endogenous DA and "newly taken-up" [3H]DA release. This effect was counteracted partially by nomifensine (10 microM). Removal of Ca++ ions from the extracellular space in the presence of the Ca++-chelator ethylene glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic acid prevented completely ouabain-elicited [3H]DA release. Lanthanum (1 mM) and cobalt (2 mM), two inorganic Ca++-entry blockers, were able to inhibit this stimulatory effect, whereas verapamil (10 microM) and nitrendipine (50 microM), two organic antagonists of the voltage-operated channel for Ca++ ions, failed to affect ouabain-induced [3H]DA release. By contrast, adriamycin (100-300 microM), a putative inhibitor of cardiac Na+-Ca++ antiporter, dose-dependently prevented ouabain-induced [3H]DA release from TIDA neurons. Finally, tetrodotoxin reduced digitalis-stimulated [3H]DA release. In conclusion, these results seem to be compatible with the idea that the inhibition of Na+,K+-adenosine triphosphatase by ouabain stimulates the release of [3H]DA from a central neuronal system like the TIDA tract and that this effect is critically dependent on the entrance of Ca++ ions into the nerve terminals of these neurons. In addition the Na+-Ca++ exchange antiporter appears to be the membrane system which transports Ca++ ions into the neuronal cytoplasm during Na+,K+-adenosine triphosphatase inhibition. The enhanced intracellular Ca++ availability triggers DA release which could occur partially through a carrier-dependent process.

  5. Effects of lithium and carbamazepine on spatial learning and depressive behavior in a rat model of bipolar disorder induced by ouabain.

    Science.gov (United States)

    Wang, Ying-Chou; Wang, En-Nan; Wang, Chia-Chuan; Huang, Chung-Lei; Huang, Andrew Chih Wei

    2013-04-01

    Lithium (LiCl) and carbamazepine (CBZ), the common mood stabilizers, are thought to be effective treatments for bipolar disorder. The aim of the present study was to investigate whether LiCl as well as CBZ has similar effects on the bipolar disorder-associated cognitive dysfunctions in rats, particularly the spatial learning and depressive responses. Adult male Wistar rats were administered intracerebroventricularly with 5μl of 10(-3)M ouabain on session 1, and then received an intraperitoneal injection of LiCl or CBZ for 4 sessions (1 session/2days). For the behavioral tests, all rats were subjected to the water maze 15min for spatial learning and the forced swimming test 5min for depression on each session. The present results showed that ouabain resulted in increased latency and longer distance traveled to reach the hidden platform in the water maze, indicating that ouabain impaired the spatial learning. However, ouabain did not affect swimming velocity in the water maze and depressive responses in the forced swimming test. LiCl treatment decreased the ouabain-enhanced latency and the total distance, but not the velocity, swam to reach the hidden platform in the water maze task. Additionally, LiCl did not result in changes of any depressive indices, such as struggling behavior, swimming behavior, and floating behavior. Likewise, CBZ did not affect any behavioral indices of spatial learning and depression. A linear regression analysis suggested that LiCl, but not CBZ, could predict the decreased latency and total distance traveled except the velocity of swimming in the water maze and depressive behaviors. In summary, the present results suggested that lithium provided a better therapeutic effect than CBZ for ouabain-caused dysfunctions of spatial learning in a rat model of bipolar disorder. Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.

  6. The key role of sodium in the ouabain-mediated potentiation of potassium-evoked catecholamine release in cat adrenal glands.

    OpenAIRE

    de Abajo, F. J.; Castro, M. A.; Sánchez-García, P.

    1989-01-01

    1. The effect of [Na]o on the catecholamine release evoked by K in ouabain pretreated, isolated adrenal glands of the cat, was investigated. 2. Reduction of [Na]o to 70, 50 and 25 mM, with sucrose as a substitute, did not modify the spontaneous catecholamine release but progressively increased the K (17.7 mM)-evoked secretory response. 3. Ouabain pretreatment (100 microM; 10 min) greatly increased the K (17.7 mM)-evoked catecholamine secretory response in glands perfused with normal Krebs. Su...

  7. Ouabain sensitive Na+/K+-pump regulates other membrane transporters in the microdomain of smooth muscle cells

    DEFF Research Database (Denmark)

    Matchkov, Vladimir; Nilsson, Holger; Aalkjær, Christian

    an interaction between the Na+/K+-pump and the Na+/Ca2+-exchanger leading to an increase in the intracellular calcium concentration  ([Ca2+]i) in discrete areas near the plasma membrane. This regulation suggests a close association of the proteins in microdomains. We have also suggested that this Na......+/K+-pump-containing microdomain is functionally linked to KATP channels via the local ion homeostasis and that this interaction can be bidirectional (1;2). [Ca2+]i in individual SMCs was imaged simultaneously with isometric force in rat mesenteric small arteries. Paired cultured rat aortic smooth muscle cells (A7r5) were used...... as a model for electrical coupling of SMC by measuring membrane capacitance (Cm). Using PCR, Western blotting and immunohistochemistry we aimed to identify the isoforms of membrane transporters involved in the suggested interaction in SMCs. SMCs were uncoupled (evaluated by inhibition of vasomotion...

  8. Nostradamus conference

    CERN Document Server

    Rössler, Otto; Snášel, Václav; Abraham, Ajith; Corchado, Emilio; Nostradamus: Modern Methods of Prediction, Modeling and Analysis of Nonlinear Systems

    2013-01-01

    This proceeding book of Nostradamus conference (http://nostradamus-conference.org) contains accepted papers presented at this event in 2012. Nostradamus conference was held in the one of the biggest and historic city of Ostrava (the Czech Republic, http://www.ostrava.cz/en), in September 2012. Conference topics are focused on classical as well as modern methods for prediction of dynamical systems with applications in science, engineering and economy. Topics are (but not limited to): prediction by classical and novel methods, predictive control, deterministic chaos and its control, complex systems, modelling and prediction of its dynamics and much more.

  9. Effect of diuretics and lithium on /sup 3/H-ouabain binding site concentration and Na,K-content in rat skeletal muscle

    Energy Technology Data Exchange (ETDEWEB)

    Noergaard, Aa.; Kjeldsen, K.

    1986-01-01

    Previous studies have shown an increase in /sup 3/H-ouabain binding sites or Na,K-pumps in vitro in cultured cells in response to incubation in low K, diuretics or lithium. However, in the present study the administration in vivo of various diuretics or lithium combined with supplementary K was not associated with any significant changes in Na,K-content or /sup 3/H-ouabain binding site concentration in rat skeletal muscle. When the diuretics were administered in combination with only the basal K requirement a decrease in both K-content and /sup 3/H-ouabain binding site concentration was seen. This indicates that the decrease in /sup 3/H-ouabain binding site concentration is not caused by these drugs per se but is secondary to the associated K-depletion. The discrepancy between the results obtained using isolated cells and rat skeletal muscles could be related to the fact that cultured cells are not subjected to the normal growth control of the intact organism. It should be emphasized that results obtained using cultured cells do not necessarily reflect processes taking place in the intact organism.

  10. Cardiac glycoside ouabain induces activation of ATF-1 and StAR expression by interacting with the α4 isoform of the sodium pump in Sertoli cells.

    Science.gov (United States)

    Dietze, Raimund; Konrad, Lutz; Shihan, Mazen; Kirch, Ulrike; Scheiner-Bobis, Georgios

    2013-03-01

    Sertoli cells express α1 and α4 isoforms of the catalytic subunit of Na(+),K(+)-ATPase (sodium pump). Our recent findings demonstrated that interactions of the α4 isoform with cardiotonic steroids (CTS) like ouabain induce signaling cascades that resemble the so-called non-classical testosterone pathway characterized by activation of the c-Src/c-Raf/Erk1/2/CREB signaling cascade. Here we investigate a possible physiological significance of the activated cascade. The results obtained in the current investigation show that the ouabain-induced signaling cascade also leads to the activation of the CREB-related activating transcription factor 1 (ATF-1) in the Sertoli cell line 93RS2 in a concentration- and time-dependent manner, as demonstrated by detection of ATF-1 phosphorylated on Ser63 in western blots. The ouabain-activated ATF-1 protein was found to localize to the cell nuclei. The sodium pump α4 isoform mediates this activation, as it is ablated when cells are incubated with siRNA to the α4 isoform. Ouabain also leads to increased expression of steroidogenic acute regulator (StAR) protein, which has been shown to be a downstream consequence of CREB/ATF-1 activation. Taking into consideration that CTS are most likely produced endogenously, the demonstrated induction of StAR expression by ouabain establishes a link between CTS, the α4 isoform of the sodium pump, and steroidogenesis crucial for male fertility and reproduction. Copyright © 2012 Elsevier B.V. All rights reserved.

  11. Consensus conferences

    DEFF Research Database (Denmark)

    Nielsen, Annika Porsborg; Lassen, Jesper

    Our results point to significant national variation both in terms of the perceived aim of consensus conferences, expectations to conference outcomes, conceptions of the roles of lay people and experts, and in terms of the way in which the role of public deliberation is interpreted. Interestingly...

  12. Amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria and its homology with the delta-subunit of the F1-ATPase of Escherichia coli.

    Science.gov (United States)

    Ovchinnikov, Y A; Modyanov, N N; Grinkevich, V A; Aldanova, N A; Trubetskaya, O E; Nazimov, I V; Hundal, T; Ernster, L

    1984-01-23

    The complete amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria is reported. The protein contains 190 amino acids and has a molecular mass of 20 967. Its structure is characterized by a concentration of charged amino acids in the two terminal segments (N 1-77 and C 128-190) of the protein, whereas its central region is more hydrophobic. The earlier reported homology of the protein with the delta-subunit of E. coli F1, based on the terminal amino acid sequences of OSCP, is further substantiated.

  13. Effects of mood stabilizers on hippocampus and amygdala BDNF levels in an animal model of mania induced by ouabain.

    Science.gov (United States)

    Jornada, Luciano K; Moretti, Morgana; Valvassori, Samira S; Ferreira, Camila L; Padilha, Peterson T; Arent, Camila O; Fries, Gabriel R; Kapczinski, Flavio; Quevedo, João

    2010-06-01

    There is a body of evidence suggesting that BDNF is involved in bipolar disorder (BD) pathogenesis. Intracerebroventricular (ICV) injection of ouabain (OUA), a specific Na(+)/K(+) ATPase inhibitor, induces hyperlocomotion in rats, and has been used as an animal model of mania. The present study aims to investigate the effects of the lithium (Li) and valproate (VPT) in an animal model of mania induced by ouabain. In the reversal model, animals received a single ICV injection of OUA or cerebrospinal fluid (aCSF). From the day following the ICV injection, the rats were treated for 6 days with intraperitoneal (IP) injections of saline (SAL), Li or VPT twice a day. In the maintenance treatment (prevention model), the rats received IP injections of Li, VPT, or SAL twice a day for 12 days. In the 7th day of treatment the animals received a single ICV injection of either OUA or aCSF. After the ICV injection, the treatment with the mood stabilizers continued for more 6 days. Locomotor activity was measured using the open-field test and BDNF levels were measured in rat hippocampus and amygdala by sandwich-ELISA. Li and VPT reversed OUA-related hyperactive behavior in the open-field test in both experiments. OUA decreased BDNF levels in first and second experiments in hippocampus and amygdala and Li treatment, but not VPT reversed and prevented the impairment in BDNF expression after OUA administration in these cerebral areas. Our results suggest that the present model fulfills adequate face, construct and predictive validity as an animal model of mania.

  14. Absence of interaction between X-rays and UV light in inducing ouabain- and thioguanine-resistant mutants in Chinese hamster cells.

    Science.gov (United States)

    Cleaver, J E

    1978-11-01

    Chinese hamster ovary cells were irradiated with X-rays at times from 0 to 17 h before being irradiated with ultraviolet (UV) light. No synergism was observed between the two radiations for the production of mutants resistant to either ouabain or 6-thioguanine. These experiments were designed to test whether X-rays induced an error-prone repair system that would increase the frequency of mutations produced by UV light, but no such system was detected.

  15. Crystal structure of the high-affinity Na+,K+-ATPase–ouabain complex with Mg2+ bound in the cation binding site

    DEFF Research Database (Denmark)

    Laursen, Mette; Yatime, Laure; Nissen, Poul

    2013-01-01

    we describe a crystal structure of the phosphorylated pig kidney Na+,K+-ATPase in complex with the CTS representative ouabain, extending to 3.4 Å resolution. The structure provides key details on CTS binding, revealing an extensive hydrogen bonding network formed by the β-surface of the steroid core......The Na+,K+-ATPase maintains electrochemical gradients for Na+ and K+ that are critical for animal cells. Cardiotonic steroids (CTSs), widely used in the clinic and recently assigned a role as endogenous regulators of intracellular processes, are highly specific inhibitors of the Na+,K+-ATPase. Here...... of ouabain and the side chains of αM1, αM2, and αM6. Furthermore, the structure reveals that cation transport site II is occupied by Mg2+, and crystallographic studies indicate that Rb+ and Mn2+, but not Na+, bind to this site. Comparison with the low-affinity [K2]E2–MgFx–ouabain structure [Ogawa et al...

  16. sup 86 Rb(K) influx and ( sup 3 H)ouabain binding by human platelets: Evidence for beta-adrenergic stimulation of Na-K ATPase activity

    Energy Technology Data Exchange (ETDEWEB)

    Turaihi, K.; Khokher, M.A.; Barradas, M.A.; Mikhailidis, D.P.; Dandona, P. (Royal Free Hospital and School of Medicine, London (England))

    1989-08-01

    Although active transport of potassium into human platelets has been demonstrated previously, there is hitherto no evidence that human platelets have an ouabain-inhibitable Na-K ATPase in their membrane. The present study demonstrates active rubidium (used as an index of potassium influx), {sup 86}Rb(K), influx into platelets, inhibitable by ouabain, and also demonstrates the presence of specific ({sup 3}H)ouabain binding by the human platelet. This {sup 86}Rb(K) influx was stimulated by adrenaline, isoprenaline, and salbutamol, but noradrenaline caused a mild inhibition. Active {sup 86}Rb(K) influx by platelets was inhibited markedly by timolol, mildly by atenolol, but not by phentolamine. Therefore, active {sup 86}Rb(K) influx in human platelets is enhanced by stimulation of beta adrenoceptors of the beta 2 subtype. The platelet may therefore replace the leukocyte in future studies of Na-K ATPase activity. This would be a considerable advantage in view of the ease and rapidity of preparation of platelets.

  17. The importance of the time of digitalization for the incidence of spasms evoked by ouabain in strips of human saphenous vein.

    Science.gov (United States)

    Zerkowski, H R; Wagner, J

    1982-10-01

    The extent of contracture induced by ouabain on preparations of the greater saphenous vein obtained from patients undergoing elective coronary bypass surgery was investigated. The medical pretreatment of the various donor patients was similar but differed with regard to the duration of preoperative digitalization ranging from several days to months. Whereas the maximal contraction induced by noradrenaline was not influenced by prior digitalization, the contracture evoked by ouabain showed a strong dependency on the duration of preoperative digitalization. In patients without or with only short-term preoperative digitalization the spasm exerted by ouabain amounted to 48.8% and 49.2%, respectively, of the maximal contraction induced by noradrenaline, and decreased to zero in patients with long-term digitalization. From this result it is concluded that, in patients after coronary artery bypass grafting who did not receive cardiac glycosides for long-term treatment, the acute administration of glycosides may be a mechanism responsible for the early occlusion of saphenous vein bypass grafts.

  18. Mendel conference

    CERN Document Server

    2015-01-01

    This book is a collection of selected accepted papers of Mendel conference that has been held in Brno, Czech Republic in June 2015. The book contents three chapters which represent recent advances in soft computing including intelligent image processing and bio-inspired robotics.: Chapter 1: Evolutionary Computing, and Swarm intelligence, Chapter 2: Neural Networks, Self-organization, and Machine Learning, and Chapter3: Intelligent Image Processing, and Bio-inspired Robotics. The Mendel conference was established in 1995, and it carries the name of the scientist and Augustinian priest Gregor J. Mendel who discovered the famous Laws of Heredity. In 2015 we are commemorating 150 years since Mendel's lectures, which he presented in Brno on February and March 1865. The main aim of the conference was to create a periodical possibility for students, academics and researchers to exchange their ideas and novel research methods.  .

  19. Conference Notification

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Roskill Information Services and Metal Events Ltd areorganizing the 2nd International Rare Earths Conference,which will be held at the Conrad Hotel in Hong Kong onFebruary 28 to March 2 2006.The program is structured tocover all the main aspects of the rare earths industry,including development of Chinese rare earth industry; trendsin rare earths demand; potential constraints on supply;research on potential capacity of rare earths supply chain.Global rare earths consumers will attend the conference.Registra...

  20. Conference Report: CAQD Conference 2013

    Directory of Open Access Journals (Sweden)

    Christina Silver

    2013-05-01

    Full Text Available Nestled on the banks of the river Lahn in central Germany, the 15th CAQD conference was held at Marburg. A beautiful provincial town, it is one of very few that was spared the bombings of WWII; now providing the perfect backdrop for meeting to discuss developments in qualitative technology. This was the second international conference in the series with more than 140 delegates from 14 countries, including: Canada, Brazil, Portugal, the UK, as well as Germany. Hosted by MAGMA, the Marburg Research Group for Methodology and Evaluation, in partnership with Philipps-University Marburg, CAQD prioritizes a user-focus which balances practical and methodological workshops with conference presentations. URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs1302249

  1. Ouabain Protects Human Renal Cells against the Cytotoxic Effects of Shiga Toxin Type 2 and Subtilase Cytotoxin

    Directory of Open Access Journals (Sweden)

    María M. Amaral

    2017-07-01

    Full Text Available Hemolytic uremic syndrome (HUS is one of the most common causes of acute renal failure in children. The majority of cases are associated with Shiga toxin (Stx-producing Escherichia coli (STEC. In Argentina, HUS is endemic and presents the highest incidence rate in the world. STEC strains expressing Stx type 2 (Stx2 are responsible for the most severe cases of this pathology. Subtilase cytotoxin (SubAB is another STEC virulence factor that may contribute to HUS pathogenesis. To date, neither a licensed vaccine nor effective therapy for HUS is available for humans. Considering that Ouabain (OUA may prevent the apoptosis process, in this study we evaluated if OUA is able to avoid the damage caused by Stx2 and SubAB on human glomerular endothelial cells (HGEC and the human proximal tubule epithelial cell (HK-2 line. HGEC and HK-2 were pretreated with OUA and then incubated with the toxins. OUA protected the HGEC viability from Stx2 and SubAB cytotoxic effects, and also prevented the HK-2 viability from Stx2 effects. The protective action of OUA on HGEC and HK-2 was associated with a decrease in apoptosis and an increase in cell proliferation. Our data provide evidence that OUA could be considered as a therapeutic strategy to avoid the renal damage that precedes HUS.

  2. Conference Hopes

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Annual conference outlines tasks for 2010 to solidify China’s economic recovery through rational investment and increasing consumptionc hina will adhere to a consistent and stable economic strategy, putting in place a proactive fiscal policy and an accommodative monetary policy for the 2010 fiscal year-the macro-economic course mapped out during China’s Central

  3. Conference proceedings

    African Journals Online (AJOL)

    abp

    2015-08-07

    Aug 7, 2015 ... African epidemiological association and 1st conference of the Cameroon society of ... International Reference Centre (CIRCB) for research on HIV/AIDS prevention and .... interests (third line regimens, clinical trials and HIV functional cure). ... sharing. Regarding Mycobacterium Tuberculosis, the efficacy of.

  4. Glucocorticoid receptor haplotypes conferring increased sensitivity (BclI and N363S) are associated with faster progression of multiple sclerosis

    NARCIS (Netherlands)

    Melief, Jeroen; Koper, Jan W; Endert, Erik; Møller, Holger J; Hamann, Jörg; Uitdehaag, Bernard M; Huitinga, I.

    2016-01-01

    As high cortisol levels are implicated in suppressed disease activity of multiple sclerosis (MS), glucocorticoid receptor (GR) polymorphisms that affect glucocorticoid (GC) sensitivity may impact on this by changing local immunomodulation or regulation of the hypothalamus-pituitary-adrenal (HPA)-axi

  5. SIGEF Conference

    CERN Document Server

    Terceño-Gómez, Antonio; Ferrer-Comalat, Joan; Merigó-Lindahl, José; Linares-Mustarós, Salvador

    2015-01-01

    This book is a collection of selected papers presented at the SIGEF conference, held at the Faculty of Economics and Business of the University of Girona (Spain), 06-08 July, 2015. This edition of the conference has been presented with the slogan “Scientific methods for the treatment of uncertainty in social sciences”. There are different ways for dealing with uncertainty in management. The book focuses on soft computing theories and their role in assessing uncertainty in a complex world. It gives a comprehensive overview of quantitative management topics and discusses some of the most recent developments in all the areas of business and management in soft computing including Decision Making, Expert Systems and Forgotten Effects Theory, Forecasting Models, Fuzzy Logic and Fuzzy Sets, Modelling and Simulation Techniques, Neural Networks and Genetic Algorithms and Optimization and Control. The book might be of great interest for anyone working in the area of management and business economics and might be es...

  6. Conference information

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ Thermag Ⅳ- The 4th International Conference on Magnetic Refrigeration at Room Temperature of IIR Refrigeration technology is widely used today. However, traditional vapor compression/expansion refrigeration technology has some disadvantages, such as low conversion efficiency of vapor compressor, and emission of the ozonosphere depletion gas and greenhouse effect gas, etc. Magnetic refrigeration is a new cooling technology with huge potential application prospect, characterized by high efficiency, energy saving and environmental friendly.

  7. Parg deficiency confers radio-sensitization through enhanced cell death in mouse ES cells exposed to various forms of ionizing radiation

    Energy Technology Data Exchange (ETDEWEB)

    Shirai, Hidenori; Fujimori, Hiroaki [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Gunji, Akemi [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Maeda, Daisuke [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Hirai, Takahisa [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Department of Radiation Oncology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 (Japan); Poetsch, Anna R. [ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Harada, Hiromi [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Yoshida, Tomoko [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minatoku, Tokyo 105-8512 (Japan); Sasai, Keisuke [Department of Radiation Oncology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 (Japan); Okayasu, Ryuichi [International Open Laboratory, National Institute of Radiological Science, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan); Masutani, Mitsuko, E-mail: mmasutan@ncc.go.jp [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)

    2013-05-24

    Highlights: •Parg{sup −/−} ES cells were more sensitive to γ-irradiation than Parp-1{sup −/−} ES cells. •Parg{sup −/−} cells were more sensitive to carbon-ion irradiation than Parp-1{sup −/−} cells. •Parg{sup −/−} cells showed defects in DSB repair after carbon-ion irradiation. •PAR accumulation was enhanced after carbon-ion irradiation compared to γ-irradiation. -- Abstract: Poly(ADP-ribose) glycohydrolase (Parg) is the main enzyme involved in poly(ADP-ribose) degradation. Here, the effects of Parg deficiency on sensitivity to low and high linear-energy-transfer (LET) radiation were investigated in mouse embryonic stem (ES) cells. Mouse Parg{sup −/−} and poly(ADP-ribose) polymerase-1 deficient (Parp-1{sup −/−}) ES cells were used and responses to low and high LET radiation were assessed by clonogenic survival and biochemical and biological analysis methods. Parg{sup −/−} cells were more sensitive to γ-irradiation than Parp-1{sup −/−} cells. Transient accumulation of poly(ADP-ribose) was enhanced in Parg{sup −/−} cells. Augmented levels of phosphorylated H2AX (γ-H2AX) from early phase were observed in Parg{sup −/−} ES cells. The induction level of p53 phophorylation at ser18 was similar in wild-type and Parp-1{sup −/−} cells and apoptotic cell death process was mainly observed in the both genotypes. These results suggested that the enhanced sensitivity of Parg{sup −/−} ES cells to γ-irradiation involved defective repair of DNA double strand breaks. The effects of Parg and Parp-1 deficiency on the ES cell response to carbon-ion irradiation (LET13 and 70 keV/μm) and Fe-ion irradiation (200 keV/μm) were also examined. Parg{sup −/−} cells were more sensitive to LET 70 keV/μm carbon-ion irradiation than Parp-1{sup −/−} cells. Enhanced apoptotic cell death also accompanied augmented levels of γ-H2AX in a biphasic manner peaked at 1 and 24 h. The induction level of p53 phophorylation at ser18 was

  8. Experimental evolution of defense against a competitive mold confers reduced sensitivity to fungal toxins but no increased resistance in Drosophila larvae

    Directory of Open Access Journals (Sweden)

    Trienens Monika

    2011-07-01

    Full Text Available Abstract Background Fungal secondary metabolites have been suggested to function as chemical defenses against insect antagonists, i.e. predators and competitors. Because insects and fungi often compete for dead organic material, insects may achieve protection against fungi by reducing sensitivity to fungal chemicals. This, in turn, may lead to increased resistance allowing insects better to suppress the spread of antagonistic but non-pathogenic microbes in their habitat. However, it remains controversial whether fungal toxins serve as a chemical shield that selects for insects that are less sensitive to toxins, and hence favors the evolution of insect resistance against microbial competitors. Results To examine the relationship between the ability to survive competition with toxic fungi, sensitivity to fungal toxins and resistance, we created fungal-selected (FS replicated insect lines by exposing Drosophila melanogaster larvae to the fungal competitor Aspergillus nidulans over 26 insect generations. Compared to unselected control lines (UC, larvae from the FS lines had higher survival rates in the presence of A. nidulans indicating selection for increased protection against the fungal antagonist. In line with our expectation, FS lines were less susceptible to the A. nidulans mycotoxin Sterigmatocystin. Of particular interest is that evolved protection against A. nidulans and Sterigmatocytin was not correlated with increased insect survival in the presence of other fungi and mycotoxins. We found no evidence that FS lines were better at suppressing the expansion of fungal colonies but observed a trend towards a less detrimental effect of FS larvae on fungal growth. Conclusion Antagonistic but non-pathogenic fungi favor insect variants better protected against the fungal chemical arsenal. This highlights the often proposed but experimentally underexplored importance of secondary metabolites in driving animal-fungus interactions. Instead of

  9. The 7B-1 mutation in tomato (Solanum lycopersicum L.) confers a blue light-specific lower sensitivity to coronatine, a toxin produced by Pseudomonas syringae pv. tomato.

    Science.gov (United States)

    Bergougnoux, Véronique; Hlavácková, Vladimíra; Plotzová, Renáta; Novák, Ondrej; Fellner, Martin

    2009-01-01

    The spontaneous mutant 7B-1 in tomato (Solanum lycopersicum=Lycopersicon esculentum) is a photoperiod-dependent male-sterile mutant previously reported as resistant to various abiotic stresses specifically under blue light. Since this finding improved the potential of 7B-1's use in breeding programmes, its susceptibility to stress induced by coronatine (COR), the phytotoxine produced by several Pseudomonas syringae strains, was assessed in this study. The 7B-1 mutant was found to be less sensitive than the corresponding wild type (WT) to COR treatment in a blue light-dependent manner. Treatment of WT and 7B-1 plants with COR induced a strong accumulation of salicylic acid (SA), jasmonic acid (JA), and abscisic acid (ABA) in hypocotyls. Interestingly, accumulation of ABA and SA in the 7B-1 mutant was distinctly greater than in WT, especially in blue light. Based on the cross-talk between SA- and JA-signalling pathways, expression analysis of NPR1 and COI1 genes, respectively involved in these pathways, was investigated in COR-stressed plants. The blue light-specific lower sensitivity of 7B-1 plants to COR was found to be associated with blue light-specific overexpression of the NPR1 gene. These data suggest that the SA-dependent NPR1-dependent pathway could be involved in the lower sensitivity of the 7B-1 mutant to COR. The role of anthocyanins and ABA accumulation during the response to COR is also discussed in the present study.

  10. Cat assay for the emetic action of digitalis and related glycosides (digitoxin, digoxin, lanatoside C, ouabain and calactin).

    Science.gov (United States)

    Parsons, J A; Summers, R J

    1971-05-01

    1. A titration assay with two end points is described for comparison of the emetic and lethal potencies of digitalis-like drugs.2. A drug was infused at constant rate to a conscious, unrestrained cat, through an indwelling venous cannula. At the moment of vomiting the cat was rapidly anaesthetized and infusion continued at the same rate until the moment of cardiac arrest.3. With very slow and very fast infusions, the emetic and lethal doses tended to rise. In the range between these extremes (which varied from drug to drug) they were independent of time.4. The observations could be accounted for by analogue computation, assuming that the drugs entered an initial pool and were distributed at finite rates to receptors in the CNS (vomiting centre) and heart.5. Half times of metabolic loss derived from this computation for digitoxin, digoxin and ouabain (17, 9.9 and 1.8 h, respectively) were in the same ratio as the threefold longer half times reported for these drugs in man.6. When measured with infusion rates in the time independent range, the ratio of lethal to emetic doses did not vary between the drugs studied. All caused vomiting at 40% of the lethal dose.7. From a review of the literature, the emetic and cardiotoxic actions of digitalis-like drugs appear inseparable and probably share a common biochemical mechanism.8. It is concluded that foreseeable improvements in digitalis-like drugs are small and would depend on the elimination of any local emetic effect on gut receptors which they may have.

  11. Ultra-high resolution polarization-sensitive optical coherence microscopy for brain imaging at 6 um, 3.4 um and 1.3 um resolution (Conference Presentation)

    Science.gov (United States)

    Wang, Hui; Akkin, Taner; Magnain, Caroline V.; Yaseen, Mohammad A.; Cramer, Avilash; Wang, Ruopeng; Sakadžic, Sava; Boas, David A.

    2016-03-01

    Neuroanatomical pathways form the basis for functional activity of brain circuits. In the past, we developed a polarization-sensitive optical coherence tomography with serial scanning to achieve large-scale brain imaging. The system was able to visualize 3D fiber tracts of ~20 um in diameter. To investigate the neuroanatomical pathways at finer scales, we have now built a polarization-maintaining fiber based ultra-high resolution polarization-sensitive optical coherence microscope (PS-OCM) at 1300 nm. The PS-OCM has an axial resolution of 3.5 um in tissue. The detection setup consists of two spectrometers, acquiring spectral interference on orthogonal polarization channels. With a single measurement, the setup generates four contrasts: reflectivity, cross-polarization, retardance and optic axis orientation. To investigate the capability of PS-OCM at different resolutions, we used three microscope objectives that yield lateral resolutions of 6.0 um, 3.4 um and 1.3 um. Blocks of formalin fixed mouse brain and human brain were scanned. The cross-polarization and retardance images clearly depict the neuronal fiber structures, which are comparable with that generated by the maximum projection of volumetric reflectivity data. The optic axis orientation quantifies the in-plane fiber orientation. With the lateral resolution of 1.3 um, the retardance contrast is weak in white matter due to the shallow depth of focus. Overall, the ultra-high resolution PS-OCM provides a new tool to reveal neuroanatomical maps in the brain at cellular resolution.

  12. Synergistic effects of graphene quantum dot sensitization and nitrogen doping of ordered mesoporous TiO2 thin films for water splitting photocatalysis(Conference Presentation)

    Science.gov (United States)

    Islam, Syed Z.; Wanninayake, Namal; Reed, Allen D.; Kim, Doo-Young; Rankin, Stephen E.

    2016-10-01

    The optical and electronic properties of TiO2 thin films provide tremendous opportunities in several applications including photocatalysis, photovoltaics and photoconductors for energy production. Despite many attractive features of TiO2, critical challenges include the innate inability of TiO2 to absorb visible light and the fast recombination of photoexcited charge carriers. In this study, we prepared ordered mesoporous TiO2 films co-modified by graphene quantum dot sensitization and nitrogen doping (GQD-N-TiO2) for hydrogen production from photoelectrochemical water splitting under visible light irradiation. First, cubic ordered mesoporous TiO2 films were prepared by a surfactant templated sol-gel method. Then, TiO2 films were treated with N2/Ar plasma for the incorporation of substitutional N atoms into the lattice of TiO2. GQDs were prepared by chemically oxidizing carbon nano-onions. The immobilization of GQDs was accomplished by reacting carboxyl groups of GQDs with amine groups of N-TiO2 developed by the prior immobilization of (3-aminopropyl)triethoxysilane (APTES). Successful immobilization of GQDs onto N-TiO2 was probed by UV-Vis, FT-IR, and scanning electron microscopy. Further, zeta potential and contact angle measurements showed enhanced surface charge and hydrophilicity, confirming the successful immobilization of GQDs. The GQD-N-TiO2, N-TiO2 and GQD-TiO2 films showed 400 times, 130 times and 8 times photocurrent enhancement, respectively, compared to TiO2 films for water splitting with a halogen bulb light source. This outstanding enhancement is attributed to the high surface area of mesoporous films and synergistic effects of nitrogen doping and GQD sensitization resulting in enhanced visible light absorption, efficient charge separation and transport.

  13. Conference Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Riveros, P. A.; Dutrizac, J. E. [Natural Resources Canada, Ottawa, ON (Canada)] [eds.

    2001-07-01

    This workshop is part of a continuing series of joint workshops organized by CANMET of Natural Resources Canada and the Research Directorate-General of the European Commission in the areas of sustainable metallurgical processing, recycling and environmental protection. The program presented at this conference also benefited from the organizational support of the Canadian Association of Recycling Industries. Over the past twenty years these workshops served as a valuable forum for the discussion of the technological issues associated with metallurgical processing, recycling and compliance with environmental regulations within the framework of sustainable development. The program this year was organized in five sessions. A total of 32 papers were presented. Session One emphasized the international dimension of modern research as illustrated by the Intelligent Manufacturing System (MIS) program. Session Two dealt with recycling, with special attention to the recycling of plastics and construction materials. Session Three was devoted to highlighting European efforts to treat chromium-bearing solutions or to find alternatives to chromium salts in surface treatment operations. Session Four emphasized primary and secondary zinc processing and the importance of energy conservation. The final session reviewed waste management practices and the utilization of waste materials. Opening addresses by representatives of the sponsoring organizations and a list of conference attendees and their affiliations are also included.

  14. NATO Conference

    CERN Document Server

    Lynn, W

    1975-01-01

    The contents of this volume involve selection, emendation and up-dating of papers presented at the NATO Conference "Mathe­ matical Analysis of Decision problems in Ecology" in Istanbul, Turkey, July 9-13, 1973. It was sponsored by the System Sciences Division of NATO directed by Dr. B. Bayraktar with local arrange­ ments administered by Dr. Ilhami Karayalcin, professor of the Department of Industrial Engineering at the Technical University of Istanbul. It was organized by A. Charnes, University professor across the University of Texas System, and Walter R.Lynn, Di­ rector of the School of Civil and Environmental Engineering at Cornell Unjversity. The objective of the conference was to bring together a group of leading researchers from the major sciences involved in eco­ logical problems and to present the current state of progress in research of a mathematical nature which might assist in the solu­ tion of these problems. Although their presentations are not herein recorded, the key­ note address of Dr....

  15. EGC Conferences

    CERN Document Server

    Ritschard, Gilbert; Pinaud, Bruno; Venturini, Gilles; Zighed, Djamel; Advances in Knowledge Discovery and Management

    This book is a collection of representative and novel works done in Data Mining, Knowledge Discovery, Clustering and Classification that were originally presented in French at the EGC'2012 Conference held in Bordeaux, France, on January 2012. This conference was the 12th edition of this event, which takes place each year and which is now successful and well-known in the French-speaking community. This community was structured in 2003 by the foundation of the French-speaking EGC society (EGC in French stands for ``Extraction et Gestion des Connaissances'' and means ``Knowledge Discovery and Management'', or KDM). This book is intended to be read by all researchers interested in these fields, including PhD or MSc students, and researchers from public or private laboratories. It concerns both theoretical and practical aspects of KDM. The book is structured in two parts called ``Knowledge Discovery and Data Mining'' and ``Classification and Feature Extraction or Selection''. The first part (6 chapters) deals with...

  16. MUSME Conference

    CERN Document Server

    Martinez, Eusebio

    2015-01-01

    This volume contains the Proceedings of MUSME 2014, held at Huatulco in Oaxaca, Mexico, October 2014. Topics include analysis and synthesis of mechanisms; dynamics of multibody systems; design algorithms for mechatronic systems; simulation procedures and results; prototypes and their performance; robots and micromachines; experimental validations; theory of mechatronic simulation; mechatronic systems; and control of mechatronic systems. The MUSME symposium on Multibody Systems and Mechatronics was held under the auspices of IFToMM, the International Federation for Promotion of Mechanism and Machine Science, and FeIbIM, the Iberoamerican Federation of Mechanical Engineering. Since the first symposium in 2002, MUSME events have been characterised by the way they stimulate the integration between the various mechatronics and multibody systems dynamics disciplines, present a forum for facilitating contacts among researchers and students mainly in South American countries, and serve as a joint conference for the ...

  17. Conference this! Lead Pipers compare conference experiences

    Directory of Open Access Journals (Sweden)

    Editorial Board

    2010-04-01

    Full Text Available As library travel budgets are increasingly slashed around the country, it’s a tough time for conference-going. In this group post, we compare notes about the conferences we’ve attended, which have been our favorites, and why. We hope this will generate creative ideas on good conferences (online or in-person to look forward to, and maybe offer [...

  18. Lithium and valproate act on the GSK-3β signaling pathway to reverse manic-like behavior in an animal model of mania induced by ouabain.

    Science.gov (United States)

    Valvassori, Samira S; Dal-Pont, Gustavo C; Resende, Wilson R; Jornada, Luciano K; Peterle, Bruna R; Machado, Alessandra Gonçalves; Farias, Hemelin Resende; de Souza, Claudio T; Carvalho, André F; Quevedo, João

    2017-05-01

    The present study aimed to investigate the effects of mood stabilizers, specifically lithium (Li) and valproate (VPA), on the PI3K/Akt signaling pathway in the brains of rats subjected to the ouabain (OUA)-induced animal model of mania. In addition, the effects of AR-A014418, a GSK-3β inhibitor, on manic-like behavior induced by OUA were evaluated. In the first experimental protocol Wistar rats received a single ICV injection of OUA or artificial cerebrospinal fluid (aCSF). From the day following ICV injection, the rats were treated for 6 days with intraperitoneal injections of saline, Li or VPA twice a day. In the second experimental protocol, rats received OUA, aCSF, OUA plus AR-A014418, or aCSF plus AR-A014418. On the 7th day after OUA injection, locomotor activity was measured using the open-field test. In addition, we analyzed the levels of p-PI3K, p-MAPK, p-Akt, and p-GSK-3β in the brain of rats by immunoblot. Li and VPA reversed OUA-related hyperactivity. OUA decreased p-PI3K, p-Akt and p-GSK-3β levels. Li and VPA improved these OUA-induced cellular dysfunctions; however, the effects of the mood stabilizers were dependent on the protein and brain region analyzed. In addition, AR-A014418 reversed the manic-like behavior induced by OUA. These findings suggest that the manic-like effects of ouabain are associated with the activation of GSK-3β, and that Li and VPA exert protective effects against OUA-induced inhibition of the GSK-3β pathway. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. 76 FR 64083 - Reliability Technical Conference; Notice of Technical Conference

    Science.gov (United States)

    2011-10-17

    ... Energy Regulatory Commission Reliability Technical Conference; Notice of Technical Conference Take notice that the Federal Energy Regulatory Commission will hold a Technical Conference on Tuesday, November 29... addressing risks to reliability that were identified in earlier Commission technical conferences....

  20. Fake/Bogus Conferences

    DEFF Research Database (Denmark)

    Asadi, Amin; Rahbar, Nader; Rezvani, Mohammad Javad

    2017-01-01

    The main objective of the present paper is to introduce some features of fake/bogus conferences and some viable approaches to differentiate them from the real ones. These fake/bogus conferences introduce themselves as international conferences, which are multidisciplinary and indexed in major sci...... scientific digital libraries. Furthermore, most of the fake/bogus conference holders offer publishing the accepted papers in ISI journals and use other techniques in their advertisement e-mails....

  1. International Conference on Physics

    CERN Document Server

    2016-01-01

    OMICS International, (conference series) the World Class Open Access Publisher and Scientific Event Organizer is hosting “International Conference on physics” which is going to be the biggest conference dedicated to Physics. The theme “Highlighting innovations and challenges in the field of Physics” and it features a three day conference addressing the major breakthroughs, challenges and the solutions adopted. The conference will be held during June 27-29, 2016 at New Orleans, USA. Will be published in: http://physics.conferenceseries.com/

  2. Facilitating Learning at Conferences

    DEFF Research Database (Denmark)

    Ravn, Ib; Elsborg, Steen

    2011-01-01

    and facilitate a variety of simple learning techniques at thirty one- and two-day conferences of up to 300 participants each. We present ten of these techniques and data evaluating them. We conclude that if conference organizers allocate a fraction of the total conference time to facilitated processes......The typical conference consists of a series of PowerPoint presentations that tend to render participants passive. Students of learning have long abandoned the transfer model that underlies such one-way communication. We propose an al-ternative theory of conferences that sees them as a forum...... for learning, mutual inspiration and human flourishing. We offer five design principles that specify how conferences may engage participants more and hence increase their learning. In the research-and-development effort reported here, our team collaborated with conference organizers in Denmark to introduce...

  3. The learning conference

    DEFF Research Database (Denmark)

    Ravn, Ib

    2007-01-01

    Purpose: To call attention to the fact that conferences for professionals rely on massive one-way communication and hence produce little learning for delegates. To introduce an alternative, the ?learning conference,? that involves delegates in fun and productive learning processes. Design....../methodology/approach: A typical full-day conference is analyzed. It has six hours of podium talk and twenty-five minutes for delegates to become involved. What model of learning can possibly lie behind this? The transfer model, which assumes learners to be empty vessels. An alternative view is that conference delegates...... are active professionals in search of inspiration, and they also want to share knowledge with their peers at the conference. A theory of the conference as a forum for mutual inspiration and human co-flourishing is proposed, as are four design principles for a learning conference: 1. Presentations must...

  4. An improved sensitive assay for the detection of PSP toxins with neuroblastoma cell-based impedance biosensor.

    Science.gov (United States)

    Zou, Ling; Wu, Chunsheng; Wang, Qin; Zhou, Jie; Su, Kaiqi; Li, Hongbo; Hu, Ning; Wang, Ping

    2015-05-15

    Paralytic shellfish poisoning (PSP) toxins are well-known sodium channel-blocking marine toxins, which block the conduction of nerve impulses and lead to a series of neurological disorders symptoms. However, PSP toxins can inhibit the cytotoxicity effect of compounds (e.g., ouabain and veratridine). Under the treatment of ouabain and veratridine, neuroblastoma cell will swell and die gradually, since veratridine causes the persistent inflow of Na(+) and ouabain inhibits the activity of Na(+)/K(+)-ATPases. Therefore, PSP toxins with antagonism effect can raise the chance of cell survival by blocking inflow of Na(+). Based on the antagonism effect of PSP toxins, we designed an improved cell-based assay to detect PSP toxins using a neuroblastoma cell-based impedance biosensor. The results demonstrated that this biosensor showed high sensitivity and good specificity for saxitoxins detection. The detection limit of this biosensor was as low as 0.03 ng/ml, which was lower than previous reported cell-based assays and mouse bioassays. With the improvement of biosensor performance, the neuroblastoma cell-based impedance biosensor has great potential to be a universal PSP screening method.

  5. Quantification of the total Na,K-ATPase concentration in atria and ventricles from mammalian species by measuring 3H-ouabain binding to intact myocardial samples. Stability to short term ischemia reperfusion

    DEFF Research Database (Denmark)

    Schmidt, T A; Svendsen, Jesper Hastrup; Haunsø, S

    2011-01-01

    Na,K-ATPase concentration was measured by vanadate facilitated 3H-ouabain binding to intact samples taken from various parts of porcine and canine myocardium. In porcine and canine heart 3H-ouabain binding site concentration in ventricles was 1.4-2.5 times larger than in atria. Evaluation of 3H.......4, respectively, and for canine heart RA and LV, both 1.2% filling (i.e., volume (ml) of incubation medium 3H-radioactivity taken up per mass unit (g wet wt.) of tissue multiplied by 100). The apparent dissociation constant (KD) was 1.4 x 10(-8) and 1.9 x 10(-8) in porcine RA and LV and 2.6 x 10(-8) and 6.1 x 10.......13, respectively, for porcine RA and LV, and factor 1.11 and 1.13 for canine RA and LV, total 3H-ouabain binding site concentration was found to be 553 +/- 74 and 1037 +/- 45 pmol/g wet wt. (means +/- SEM, n = 5) in porcine RA and LV, and 569 +/- 37 and 1410 +/- 40 pmol/g wet wt. (means +/- SEM, n = 5...

  6. International Cryocooler Conference

    CERN Document Server

    Cryocoolers 13

    2005-01-01

    This is the 13th volume in the conference series. Over the years the International Cryocoolers Conference has become the preeminent worldwide conference for the presentation of the latest developments and test experiences with cryocoolers. The typical applications of this technology include cooling space and terrestrial infrared focal plane arrays, space x-ray detectors, medical applications, and a growing number of high-temperature super-capacitor applications.

  7. Expectations for Cancun Conference

    Institute of Scientific and Technical Information of China (English)

    DING ZHITAO

    2010-01-01

    Compared with the great hopes raised by the Copenhagen Climate Conference in 2009, the 2010 UN Climate Change Conference in Cancun aroused fewer expectations. However, the international community is still waiting for a positive outcome that will benefit humankind as a whole. The Cancun conference is another important opportunity for all the participants to advance the Bali Road Map negotiations after last year's meeting in Copenhagen, which failed to reach a legally binding treaty for the years beyond 2012.

  8. Preparation and identification of the fragment of rabbit's polyclonal antibodies against human ouabain%抗哇巴因多克隆抗体F(ab)2 片段的研制及鉴定

    Institute of Scientific and Technical Information of China (English)

    张明娟; 吕卓人; 袁育康; 贺浪冲; 王颢

    2000-01-01

    Objective Anti-ouabain polyclonal antibody was digested with pepsin to prepare F fragment in order to find the base of reshaped antibody. Methods Anti-ouabain polyclonal antibody was prepared by immunizing rabbits. Then anti-ouabain polyclonal antibody was digested under different conditions. The reactants were analyzed and purified by High Performance Size Ex-clude Chromatography (HPSEC). The immune activities were detected and identified by Enzyme Linked Immunosorbent Assay (ELISA). Results The proper reactive conditions for preparation of anti-ouabain polyclonal antibody F(ab)2 fragment with pepsin were established. The eligible dose of pepsin, by which 100mg anti-onabain polyclonal antibody was digested better, was 2mg. The eligible digesuve time was 18h. The value of pH of reactive system was 3.0. Conclusion The active F fragment of anti-ouabain polyclonal antibody can be obtained with pepsin. The method is simple and feasible.%目的用胃蛋白酶酶解抗哇巴因多克隆抗体,制备出片段,为改型抗体的研究提 供依据。方法免疫家兔制备出抗哇巴因多克隆抗体,并用胃蛋白酶分别在不同的酶解条件下消化抗体,继之用高效液相排阻色谱法(HPSEC)对其进行分析、纯化,采用酶联免疫吸附法(ELISA)检测、鉴定其活性。结果用胃蛋白酶2mg/100mg抗哇巴因多克隆抗体,消化18h,反应体系的pH3.0时,可获得片段。结论用胃蛋白酶酶解抗哇巴因多克隆抗体是研制出有活性的片段的有效方法。

  9. Endogenous Ouabain: An Old Cardiotonic Steroid as a New Biomarker of Heart Failure and a Predictor of Mortality after Cardiac Surgery

    Directory of Open Access Journals (Sweden)

    Marco Simonini

    2015-01-01

    Full Text Available Cardiovascular diseases remain the main cause of mortality and morbidity worldwide; primary prevention is a priority for physicians. Biomarkers are useful tools able to identify high-risk individuals, guide treatments, and determine prognosis. Our aim is to investigate Endogenous Ouabain (EO, an adrenal stress hormone with hemodynamic effects, as a valuable biomarker of heart failure. In a population of 845 patients undergoing elective cardiac surgery, we have investigated the relationships between EO and echocardiography parameters/plasmatic biomarker of cardiac function. EO was found to be correlated negatively with left ventricular EF (p=0.001, positively with Cardiac End-Diastolic Diameter (p=0.047, and positively with plasmatic NT-proBNP level (p=0.02. Moreover, a different plasmatic EO level (both preoperative and postoperative was found according to NYHA class (p=0.013. All these results have been replicated on an independent cohort of patients (147 subjects from US. Finally, a higher EO level in the immediate postoperative time was indicative of a more severe cardiological condition and it was associated with increased perioperative mortality risk (p=0.023 for 30-day morality. Our data suggest that preoperative and postoperative plasmatic EO level identifies patients with a more severe cardiovascular presentation at baseline. These patients have a higher risk of morbidity and mortality after cardiac surgery.

  10. Conference proceedings ISES 2014

    DEFF Research Database (Denmark)

    Christensen, Janne Winther; Peerstrup Ahrendt, Line; Malmkvist, Jens

    The 10th Internatinal Equitation Science Conference is held i Denmark from August 6th - 9th 2014. This book of proceedings contaions abstracts of 35 oral and 57 poster presentations within the conference themes Equine Stress, Learning and Training as well as free papers.......The 10th Internatinal Equitation Science Conference is held i Denmark from August 6th - 9th 2014. This book of proceedings contaions abstracts of 35 oral and 57 poster presentations within the conference themes Equine Stress, Learning and Training as well as free papers....

  11. Second international conference on isotopes. Conference proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Hardy, C.J. [ed.

    1997-10-01

    The Second International Conference on Isotopes (2ICI) was hosted by the Australian Nuclear Association in Sydney, NSW, Australia. The Theme of the Second Conference: Isotopes for Industry, Health and a Better Environment recognizes that isotopes have been used in these fields successfully for many years and offer prospects for increasing use in the future. The worldwide interest in the use of research reactors and accelerators and in applications of stable and radioactive isotopes, isotopic techniques and radiation in industry, agriculture, medicine, environmental studies and research in general, was considered. Other radiation issues including radiation protection and safety were also addressed. International and national overviews and subject reviews invited from leading experts were included to introduce the program of technical sessions. The invited papers were supported by contributions accepted from participants for oral and poster presentation. A Technical Exhibition was held in association with the Conference. This volume contains the full text or extended abstracts of papers number 61- to number 114

  12. Context Sensitive Health Informatics

    DEFF Research Database (Denmark)

    Healthcare information technologies are now routinely deployed in a variety of healthcare contexts. These contexts differ widely, but the smooth integration of IT systems is crucial, so the design, implementation, and evaluation of safe, effective, efficient and easy to adopt health informatics...... involves careful consideration of both human and organizational factors. This book presents the proceedings of the Context Sensitive Health Informatics (CSHI) conference, held in Copenhagen, Denmark, in August 2013. The theme of this year’s conference is human and sociotechnical approaches. The Human...

  13. Minoxidil opens mitochondrial KATP channels and confers cardioprotection

    Science.gov (United States)

    Sato, Toshiaki; Li, Yulong; Saito, Tomoaki; Nakaya, Haruaki

    2003-01-01

    ATP-sensitive potassium channel in the mitochondrial inner membrane (mitoKATP channel) rather than in the sarcolemma (sarcKATP channel) appears to play an important role in cardioprotection. We examined the effect of minoxidil, a potent antihypertensive agent and hair growth stimulator, on sarcKATP and mitoKATP channels in guinea-pig ventricular myocytes. Minoxidil activated a glybenclamide-sensitive sarcKATP channel current in the whole-cell recording mode with an EC50 of 182.6 μM. Minoxidil reversibly increased the flavoprotein oxidation, an index of mitoKATP channel activity, in a concentration-dependent manner. The EC50 for mitoKATP channel activation was estimated to be 7.3 μM; this value was notably ≈25-fold lower than that for sarcKATP channel activation. Minoxidil (10 μM) significantly attenuated the ouabain-induced increase of mitochondrial Ca2+ concentration, which was measured by loading cells with rhod-2 fluorescence. Furthermore, pretreatment with minoxidil (10 μM) before 20-min no-flow ischaemia significantly improved the recovery of developed tension measured after 60 min of reperfusion in coronary perfused guinea-pig ventricular muscles. These cardioprotective effects of minoxidil were completely abolished by the mitoKATP channel blocker 5-hydroxydecanoate (500 μM). Our results indicate that minoxidil exerts a direct cardioprotective effect on heart muscle cells, an effect mediated by the selective activation of mitoKATP channels. PMID:14691056

  14. Radiation`96. Conference handbook

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-31

    The conference program includes eight invited lectures which cover a range of contemporary topics in radiation science and technology. In addition, thirty-two oral papers were presented, along with forty-five posters. The conference handbook contains one-page precis or extended abstracts of all presentations, and is a substantial compendium of current radiation research in Australia.

  15. Expectations for Cancun Conference

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Compared with the great hopes raised by the Copenhagen Climate Conference in 2009, the 2010 UN Climate Change Conference in Cancun aroused fewer expectations. However, the international community is still waiting for a positive outcome that will benefit humankind as a whole.

  16. 3rd Cryocooler Conference

    CERN Document Server

    Louie, Berverly; McCarthy, Sandy

    1985-01-01

    Cryocoolers 3 documents the output of the Third Cryocooler Conference, held at the National Bureau of Standards, Boulder, Colorado, on September 17-18, 1984. About 140 people from 10 countries attended the conference representing industry, government, and academia. A total of 26 papers were presented orally at the conference and all appear in written form in the proceedings. The focus of this conference was on small cryocoolers in the temperature range of 4 - 80 K. Mechanical and nonmechanical types are discussed in the various papers. Applications of these small cryocoolers include the cooling of infrared detectors, cryopumps, small superconducting devices and magnets, and electronic devices. The conference proceedings reproduced here was published by the National Bureau of Standards in Boulder, Colorado as NBS Special Publication #698.

  17. Second international conference on isotopes. Conference proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Hardy, C.J. [ed.

    1997-10-01

    The Second International Conference on Isotopes (2ICI) was hosted by the Australian Nuclear Association in Sydney, NSW, Australia. The Theme of the Second Conference: Isotopes for Industry, Health and a Better Environment recognizes that isotopes have been used in these fields successfully for many years and offer prospects for increasing use in the future. The worldwide interest in the use of research reactors and accelerators and in applications of stable and radioactive isotopes, isotopic techniques and radiation in industry, agriculture, medicine, environmental studies and research in general, was considered. Other radiation issues including radiation protection and safety were also addressed. International and national overviews and subject reviews invited from leading experts were included to introduce the program of technical sessions. The invited papers were supported by contributions accepted from participants for oral and poster presentation. A Technical Exhibition was held in association with the Conference. This volume contains the foreword, technical program, the author index and of the papers (1-60) presented at the conference.

  18. Conference Report: Wyoming Invitational Conference on Instructional Applications of Computers.

    Science.gov (United States)

    Kansky, Bob

    This report: (1) describes the organization of an invitational conference aimed at gathering direction from classroom teachers regarding instructional applications of computers; (2) provides copies of all materials used in organizing such a conference; and (3) reports the results of the conference in terms of conference products (resolutions,…

  19. Multiwavelength Emission from Blazars – Conference Summary

    Indian Academy of Sciences (India)

    Meg Urry

    2011-03-01

    Presentations at the Guangzhou Conference on Multiwave-length Emission from Blazars confirmed our understanding of blazars as relativistic jets closely aligned with the line of sight. Powerful new studies have been enabled by the Fermi gamma-ray satellite and new ground-based TeV facilities, which are an order of magnitude more sensitive than their predecessors. Combining gamma-ray data with VLBA radio and with optical/IR photometry has shed new light on the emission mechanisms and the jet geometry. This conference summary sets the context for the 4th blazar conference and presents some of the highlights from the meeting, as well as the questions that remain outstanding.

  20. The learning conference

    DEFF Research Database (Denmark)

    Ravn, Ib

    The typical one-day conference attended by managers or professionals in search of inspiration is packed with PowerPoint presentations and offers little opportunity for involvement or knowledge sharing. Behind the conventional conference format lurks the transfer model of learning, which finds...... little support amongst serious students of learning. The professional conference as a forum for knowledge sharing is in dire need of a new learning theory and a more enlightened practice. The notion of human flourishing is offered as basis for theory, and four simple design principles for the so......-called “learning conference” are proposed: People go to conferences to 1. get concise input, 2. interpret it in the light of their ongoing concerns, 3. talk about their current projects and 4. meet the other attendees and be inspired by them. Six practical techniques that induce attendees to do these things...

  1. Statewide Professional Development Conference

    Directory of Open Access Journals (Sweden)

    Paul V. Bredeson

    2000-02-01

    Full Text Available In an environment increasingly skeptical of the effectiveness of large-scale professional development activities, this study examines K-12 educators' reasons for participating and beliefs in the utility in a large-scale professional development conference. Pre- and post-conference surveys revealed that while financial support played a significant role in educators' ability to participate, they were drawn to the conference by the promise to learn substantive issues related to, in this case, performance assessment—what it means, how to implement it, and how to address community concerns. In spite of the conference's utility as a means to increase awareness of critical issues and to facilitate formal and informal learning, well conceived linkages to transfer new knowledge to the school and classroom were lacking.

  2. Tackling conference carbon footprints

    Science.gov (United States)

    Grozier, Jim

    2016-12-01

    In reply to Margaret Harris's Lateral Thoughts article "Putting my foot down", which discussed the challenges of attending a conference with a physical disability (October p76) and a subsequent letter by Anna Wood (November p18).

  3. The learning conference

    DEFF Research Database (Denmark)

    Ravn, Ib

    2007-01-01

    are described: Individual reflection, the buzz dyad, ?You have won two consultants, free of charge?, facilitated group work, the knowledge exchange, and lunch with gaffer tape. Originality/value: This paper introduces modern learning theory and techniques into an educational context which has resisted......Purpose: To call attention to the fact that conferences for professionals rely on massive one-way communication and hence produce little learning for delegates. To introduce an alternative, the ?learning conference,? that involves delegates in fun and productive learning processes. Design....../methodology/approach: A typical full-day conference is analyzed. It has six hours of podium talk and twenty-five minutes for delegates to become involved. What model of learning can possibly lie behind this? The transfer model, which assumes learners to be empty vessels. An alternative view is that conference delegates...

  4. The learning conference

    DEFF Research Database (Denmark)

    Ravn, Ib

    2007-01-01

    are described: Individual reflection, the buzz dyad, ?You have won two consultants, free of charge?, facilitated group work, the knowledge exchange, and lunch with gaffer tape. Originality/value: This paper introduces modern learning theory and techniques into an educational context which has resisted......Purpose: To call attention to the fact that conferences for professionals rely on massive one-way communication and hence produce little learning for delegates. To introduce an alternative, the ?learning conference,? that involves delegates in fun and productive learning processes. Design....../methodology/approach: A typical full-day conference is analyzed. It has six hours of podium talk and twenty-five minutes for delegates to become involved. What model of learning can possibly lie behind this? The transfer model, which assumes learners to be empty vessels. An alternative view is that conference delegates...

  5. DNA sequencing conference, 2

    Energy Technology Data Exchange (ETDEWEB)

    Cook-Deegan, R.M. [Georgetown Univ., Kennedy Inst. of Ethics, Washington, DC (United States); Venter, J.C. [National Inst. of Neurological Disorders and Strokes, Bethesda, MD (United States); Gilbert, W. [Harvard Univ., Cambridge, MA (United States); Mulligan, J. [Stanford Univ., CA (United States); Mansfield, B.K. [Oak Ridge National Lab., TN (United States)

    1991-06-19

    This conference focused on DNA sequencing, genetic linkage mapping, physical mapping, informatics and bioethics. Several were used to study this sequencing and mapping. This article also discusses computer hardware and software aiding in the mapping of genes.

  6. Ranking Operations Management Conferences

    NARCIS (Netherlands)

    Steenhuis, Harm-Jan; Bruijn, de Erik Joost; Gupta, Sushil; Laptaned, U.

    2007-01-01

    Several publications have appeared in the field of Operations Management which rank Operations Management related journals. Several ranking systems exist for journals based on , for example, perceived relevance and quality, citation, and author affiliation. Many academics also publish at conferences

  7. EVOLVE 2014 International Conference

    CERN Document Server

    Tantar, Emilia; Sun, Jian-Qiao; Zhang, Wei; Ding, Qian; Schütze, Oliver; Emmerich, Michael; Legrand, Pierrick; Moral, Pierre; Coello, Carlos

    2014-01-01

    This volume encloses research articles that were presented at the EVOLVE 2014 International Conference in Beijing, China, July 1–4, 2014.The book gathers contributions that emerged from the conference tracks, ranging from probability to set oriented numerics and evolutionary computation; all complemented by the bridging purpose of the conference, e.g. Complex Networks and Landscape Analysis, or by the more application oriented perspective. The novelty of the volume, when considering the EVOLVE series, comes from targeting also the practitioner’s view. This is supported by the Machine Learning Applied to Networks and Practical Aspects of Evolutionary Algorithms tracks, providing surveys on new application areas, as in the networking area and useful insights in the development of evolutionary techniques, from a practitioner’s perspective. Complementary to these directions, the conference tracks supporting the volume, follow on the individual advancements of the subareas constituting the scope of the confe...

  8. Multiphoton processes: conference proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Lambropoulos, P.; Smith, S.J. (eds.)

    1984-01-01

    The chapters of this volume represent the invited papers delivered at the conference. They are arranged according to thermatic proximity beginning with atoms and continuing with molecules and surfaces. Section headings include multiphoton processes in atoms, field fluctuations and collisions in multiphoton process, and multiphoton processes in molecules and surfaces. Abstracts of individual items from the conference were prepared separately for the data base. (GHT)

  9. 2nd SUMO Conference

    CERN Document Server

    Weber, Melanie

    2015-01-01

    This contributed volume contains the conference proceedings of the Simulation of Urban Mobility (SUMO) conference 2014, Berlin. The included research papers cover a wide range of topics in traffic planning and simulation, including open data, vehicular communication, e-mobility, urban mobility, multimodal traffic as well as usage approaches. The target audience primarily comprises researchers and experts in the field, but the book may also be beneficial for graduate students.  

  10. Conference scene: DGVS spring conference 2009.

    Science.gov (United States)

    Kolligs, Frank Thomas

    2009-10-01

    The 3rd annual DGVS Spring Conference of the German Society for Gastroenterology (Deutsche Gesellschaft für Verdauungs- und Stoffwechselkrankheiten) was held at the Seminaris Campus Hotel in Berlin, Germany, on 8-9 May, 2009. The conference was organized by Roland Schmid and Matthias Ebert from the Technical University of Munich, Germany. The central theme of the meeting was 'translational gastrointestinal oncology: towards personalized medicine and individualized therapy'. The conference covered talks on markers for diagnosis, screening and surveillance of colorectal cancer, targets for molecular therapy, response prediction in clinical oncology, development and integration of molecular imaging in gastrointestinal oncology and translational research in clinical trial design. Owing to the broad array of topics and limitations of space, this article will focus on biomarkers, response prediction and the integration of biomarkers into clinical trials. Presentations mentioned in this summary were given by Matthias Ebert (Technical University of Munich, Germany), Esmeralda Heiden (Epigenomics, Berlin, Germany), Frank Kolligs (University of Munich, Germany), Florian Lordick (University of Heidelberg, Germany), Hans Jorgen Nielsen (University of Copenhagen, Denmark), Anke Reinacher-Schick (University of Bochum, Germany), Christoph Röcken (University of Berlin, Germany), Wolff Schmiegel (University of Bochum, Germany) and Thomas Seufferlein (University of Halle, Germany).

  11. Conference Report: The BPS Annual Conference 2004

    Directory of Open Access Journals (Sweden)

    Irina Roncaglia

    2004-05-01

    Full Text Available In this article I will review four papers presented at the British Psychological Society Annual Conference held this year in London held over a 3 day period. The Conference included a variety of scientific presentations and discussions through symposia, roundtable discussions, single papers and poster sessions. Although numerous papers took an experimental approach, few applied any type of qualitative methodology. The topics covered within the different psychological disciplines spanned from early childhood through old age; I have chosen four papers that covered a life course perspective and took into consideration clinical issues as well. The first paper discusses a grounded theory approach used to analyse a play therapy session between therapist and child. The second review reports some recent findings in the way the brains of people on the autistic spectrum disorder might function. The third paper discusses positive psychology and how such an emerging movement has influenced new research in the field. The last paper reviewed will discuss the issue of the ageing process, and I will present some arguments related to the useful application of qualitative methodologies within this area of research. In conclusion, I will highlight some personal reflections on the Conference and the need for a greater balance between qualitative and quantitative methodologies to be used in collaboration rather than as antagonists. URN: urn:nbn:de:0114-fqs0402176

  12. Effects of hydrogen sulfide on ouabain-induced delayed after-depolarization and triggered activity in guinea pig papillary muscles%硫化氢对哇巴因诱发的豚鼠乳头肌迟后去极化及触发活动的影响

    Institute of Scientific and Technical Information of China (English)

    刘慧霞; 许萌; 郭琪; 金胜; 薛红梅; 武宇明

    2011-01-01

    Objective To explore the effects of hydrogen sulfide (H2S) on delayed afterdepolarization (DAD) and triggered activity induced by ouabain in male guinea pig papillary muscles and to elucidate the underlying mechanisms.Methods An intracellular microelectrode was used to record the patterns of DAD and triggered activity by K-H solution containing ouabain and a high concentration of calcium ion.The latent period,amplitude,duration of DAD and incidence of triggered activity were observed under a pre-treatment with different concentrations of NaHS (donor of H2S).The effects of glibenclamide,Bay K8644 and NG-nitro-L-arginine methyl ester (L-NAME) pretreatment on the actions of H2S were also studied.Results NaHS ( 100,200 μmoL/L) prolonged the latent period of DAD from( 12.0 ± 1.0) min to ( 19.9 ± 1.6) min ( P < 0.05 ),( 23.7 ± 1.3 ) min ( P < 0.01 ),decreased the altitude of DAD from ( 11.47 ±0.74) mV to (6.47 ±0.33) mY,(5.65 ±0.26) mV ( both P <0.01 ),shortened the duration of DAD from (205 ± 11 ) ms to ( 173 ± 10) ms and ( 134 ± 7 ) ms ( both P < 0.05 ).The occurrence of triggered activity was inhibited from 5 samples to 4,2 and 1 sample in 6 samples.A pretreatment of adenosine triphosphate ( ATP)-sensitive potassium channel ( KATP ) blocker glibenclamide partially blocked the preventive effects of H2S on ouabain-induced DAD and triggered activity.The effects of H2S were completely blocked by L-type calcium channel agonist Bay K8644 (0.25 μmol/L).However a pretreatment of L-NAME ( 1 mmol/L),a nitric oxide (NO) synthase inhibitor,showed no effects on H2S.Conclusion H2S inhibits the ouabain-induced DAD and triggered activity in guinea pig papillary muscles.The opening of KATP channel with a reduced influx of calcium ion may be involved in the protective effects of H2S.%目的 探讨硫化氢(H2S)对哇巴因诱发的豚鼠乳头肌迟后去极化(DAD)及触发活动的影响及其可能的机制.方法 应用细胞内玻璃微电极技术记录含哇

  13. 2nd Bozeman Conference

    CERN Document Server

    Lund, John

    1991-01-01

    This volume contains a collection of papers delivered by the partici­ pants at the second Conference on Computation and Control held at Mon­ tana State University in Bozeman, Montana from August 1-7, 1990. The conference, as well as this proceedings, attests to the vitality and cohesion between the control theorist and the numerical analyst that was adver­ tised by the first Conference on Computation and Control in 1988. The proceedings of that initial conference was published by Birkhiiuser Boston as the first volume of this same series entitled Computation and Control, Proceedings of the Bozeman Conference, Bozeman, Montana, 1988. Control theory and numerical analysis are both, by their very nature, interdisciplinary subjects as evidenced by their interaction with other fields of mathematics and engineering. While it is clear that new control or es­ timation algorithms and new feedback design methodologies will need to be implemented computationally, it is likewise clear that new problems in computation...

  14. Effects and Mechanism of Hydrogen Sulfide on Ouabain-induced Arrhythmias%硫化氢抗哇巴因诱发大鼠触发性心律失常作用的观察

    Institute of Scientific and Technical Information of China (English)

    刘慧霞; 李琳; 陈宝琅; 闫长虹; 王旭; 孔晓霞; 吕昆

    2015-01-01

    目的 观察硫化氢对哇巴因诱发触发性心律失常的作用, 并进一步探讨其可能的作用机制. 方法 采用SD大鼠, 建立离体心脏Langendorff主动脉逆行灌流系统. 应用1μmol/L哇巴因灌流心脏诱发室性心律失常, 分别观察50, 100, 200 μmol/L硫氢化钠 ( H2 S的供体) 对心律失常的影响, 全程记录心电图的变化. 按照Na+-K+-ATP酶、 Ca2+-Mg2+-ATP酶活性测定试剂盒检测各组心肌细胞Na+-K+-ATP酶、 Ca2+-Mg2+-ATP酶活性. 结果 哇巴因可诱发出稳定的室性心律失常, 硫氢化钠可浓度依赖性抑制哇巴因诱发的期前收缩, 降低室速和室颤的发生率 ( P<0. 05 ) , 哇巴因抑制Na+-K+-ATP酶、 Ca2+-Mg2+-ATP酶活性, 硫氢化钠增加其活性. 结论 硫化氢有抗心律失常的作用, 其机制可能与增强Na+-K+-ATP酶、 Ca2+-Mg2+-ATP酶的活性有关.%Objective To observe hydrogen sulfide′s effect on ouabain-induced arrhythmias and further explore its possible mech-anism. Methods Healthy Sprague-Dawley rats were used to establish a Langen-dorff retrograde perfusion system. Arrhythmia was e-licited in isolated rat hearts by the perfusion of 1 μmol/L Ouabain, sodium hydrosulfide (50, 100, 200 μmol/L) was given immedi-ately after the development of first sinus arrhythmia respectively. The arrhythmias were monitored and compared by simultaneous ECG recording, and the Na+-K+-ATP and Ca2+-Mg2+-ATP of myocardial cell in each group were examined. Results Ouabain could in-duce stable ventricular arrhythmias. Sodium hydrosulfide inhibited ouabain-induced premature ventricular beats ( PVB) in a concen-tration-dependent manner, and decreased the incidents of ventricular tachycardia (VT) and ventricular fibrillation (VF) (P<0. 05). Ouabain inhibited the activity of Na+-K+-ATP and Ca2+-Mg2+-ATP, while sodium hydrosulfide increased the activity. Conclusion Hydrogen Sulfide can perform anti-arrhythmic function and its mechanism may be related to the increasing

  15. 5th ESCOM Conference

    DEFF Research Database (Denmark)

    Ridder, Hanne Mette Ochsner

    2003-01-01

    What happens when a music therapist participates in a conference of the European Society for the Cognitive Sciences of Music? Well, she seems to get a lot of inspiration and sees very interesting and useful research, - and sometimes she is astonished about which kind of research some people are i...... are investigating time and money in. In the following you will find thoughts and impressions from the conference where there were a huge number of presentations with a common theme on cognitive science of music, and here seen from a music therapeutic perspective....

  16. PHYSICS FOR HEALTH: CONFERENCE

    CERN Multimedia

    2016-01-01

    ICTR-PHE 2016 - International Conference on Translational Research in Radio-Oncology and Physics for Health -, co organized by CERN, aims at developing new strategies to better diagnose and treat cancer, by uniting biology and physics with clinics. Through the various sessions and symposia, the scientific programme offers the delegates the opportunity to discuss, in a friendly atmosphere, the latest progress in physics breakthroughs for health applications. The third edition of this conference took place at CICG (Centre International de Conférence Genève) from 15 to 19 Feb 2016.

  17. Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population.

    Science.gov (United States)

    Cockram, James; Scuderi, Alice; Barber, Toby; Furuki, Eiko; Gardner, Keith A; Gosman, Nick; Kowalczyk, Radoslaw; Phan, Huyen P; Rose, Gemma A; Tan, Kar-Chun; Oliver, Richard P; Mackay, Ian J

    2015-09-28

    The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world's most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1. Copyright © 2015 Cockram et al.

  18. 78 FR 27963 - Reliability Technical Conference; Notice of Technical Conference

    Science.gov (United States)

    2013-05-13

    ... Energy Regulatory Commission Reliability Technical Conference; Notice of Technical Conference Take notice that the Federal Energy Regulatory Commission will hold a Technical Conference on Tuesday, July 9, 2013... to the webcast. The Capitol Connection provides technical support for webcasts and offers the...

  19. International conference on string theory

    CERN Document Server

    2016-01-01

    The Strings conference is an annual event that brings the entire string theory community together. Since the 1980s, it has grown to be the largest and most important conference in the field. The aim is to review recent developments in string theory and to stimulate scientific exchanges among the participants. This is the second Strings conference organised in Beijing, after Strings 2006. Following the tradition, besides scientific talks, the conference will also include some public lectures open to a general audience.

  20. On the Conference Circuit.

    Science.gov (United States)

    Tyckoson, David A.

    1987-01-01

    Summarizes three conference presentations on the effects of the economic climate on academic libraries in Iowa. These presentations focused on the impact of austerity budgets on collection development, library services and personnel, and possible management approaches to retrenchment in these areas. (CLB)

  1. Metabolic Engineering X Conference

    Energy Technology Data Exchange (ETDEWEB)

    Flach, Evan [American Institute of Chemical Engineers

    2015-05-07

    The International Metabolic Engineering Society (IMES) and the Society for Biological Engineering (SBE), both technological communities of the American Institute of Chemical Engineers (AIChE), hosted the Metabolic Engineering X Conference (ME-X) on June 15-19, 2014 at the Westin Bayshore in Vancouver, British Columbia. It attracted 395 metabolic engineers from academia, industry and government from around the globe.

  2. CONFERENCE OF YOUNG SCIENTISTS

    Directory of Open Access Journals (Sweden)

    article editorial

    2008-01-01

    Full Text Available A conference of young scientists dedicated to the 245th anniversary of the Scientific Center of Children's Health was held on September 12, 2008.Speakers at the conference were young professionals working at SCCH of RAMS, Sechenov Moscow Medical Academy, Institute of Pediatric Hematology, Oncology, and Immunology. During the conference the poster session was held in which young scientists of the Centre took part. Presentations of participants were evaluated by the members of the Academic Council of SCCH, leading specialists of the Center. Gifts to the winners of the conference were provided by "Procter & Gamble".Winners and awardees:I place: Chistyakova V.P. - Clinical intern at SCCH of RAMS;II place: Gudkova E.Yu. - Researcher at the Department of Rheumatology of SCCH of RAMS;III place: Darmanyan A.S. - Researcher at the Department of diagnostics and rehabilitation treatment of SCCH of RAMS.Best poster paper: Plyakin V.A. - Researcher at Surgical Department NTSZD RAMS.Audience Award: Tikhomirova E.A. - Researcher at the Department of functional diagnostics of SCCH of RAMS.

  3. Graphics Conference Calendar

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    1. The 13th International Conference in Central Europe on Computer Graphics, Visualization and Computer Vision'2005, University of West Bohemia, Campus-Bory Plzen (very close to Prague, the capital of the Czech Republic)Czech Republic, January 31 - February 4, 2005. http://wscg.zcu.cz, skala@kiv.zcu.cz

  4. International Nuclear Physics Conference

    CERN Document Server

    2016-01-01

    We are pleased to announce that the 26th International Nuclear Physics Conference (INPC2016) will take place in Adelaide (Australia) from September 11-16, 2016. The 25th INPC was held in Firenze in 2013 and the 24th INPC in Vancouver, Canada, in 2010. The Conference is organized by the Centre for the Subatomic Structure of Matter at the University of Adelaide, together with the Australian National University and ANSTO. It is also sponsored by the International Union of Pure and Applied Physics (IUPAP) and by a number of organisations, including AUSHEP, BNL, CoEPP, GSI and JLab. INPC 2016 will be held in the heart of Adelaide at the Convention Centre on the banks of the River Torrens. It will consist of 5 days of conference presentations, with plenary sessions in the mornings, up to ten parallel sessions in the afternoons, poster sessions and a public lecture. The Conference will officially start in the evening of Sunday 11th September with Registration and a Reception and will end late on the afternoon of ...

  5. Creating Better Satellite Conferences.

    Science.gov (United States)

    Horner, Tommy

    1998-01-01

    Presents four ways to improve broadcasts of company satellite conferences, including creative site selection (using facilities at educational institutions rather than hotel rooms); creative programming (using graphics and other interruptions to break up lectures or speeches); creative crew selection; and creative downlink site activities (to…

  6. 2002 NASPSA Conference Abstracts.

    Science.gov (United States)

    Journal of Sport & Exercise Psychology, 2002

    2002-01-01

    Contains abstracts from the 2002 conference of the North American Society for the Psychology of Sport and Physical Activity. The publication is divided into three sections: the preconference workshop, "Effective Teaching Methods in the Classroom;" symposia (motor development, motor learning and control, and sport psychology); and free…

  7. Report on the Conference.

    Science.gov (United States)

    Brown, Ralph S.

    1983-01-01

    The themes of the 1982 annual conference of the American Association of University Professors are outlined. They include the importance of planning, selective versus across-the-board retrenchment strategies, definitions and problems of financial exigency, program reduction, and affirmative action claims. (MSE)

  8. Grammar! A Conference Report.

    Science.gov (United States)

    King, Lid, Ed.; Boaks, Peter, Ed.

    Papers from a conference on the teaching of grammar, particularly in second language instruction, include: "Grammar: Acquisition and Use" (Richard Johnstone); "Grammar and Communication" (Brian Page); "Linguistic Progression and Increasing Independence" (Bernardette Holmes); "La grammaire? C'est du bricolage!" ("Grammar? That's Hardware!") (Barry…

  9. Indico CONFERENCE: Define the Programme

    CERN Document Server

    CERN. Geneva; Ferreira, Pedro

    2017-01-01

    In this tutorial you are going to learn how to define the programme of a conference in Indico. The program of your conference is divided in different “tracks”. Tracks represent the subject matter of the conference, such as “Online Computing”, “Offline Computing”, and so on.

  10. ALA Conference 2009: Chicago Hope

    Science.gov (United States)

    Berry, John N., III

    2009-01-01

    There is joy among those who have the funds to go to Chicago for the American Library Association (ALA) annual conference, July 9-15. Every librarian knows there is nothing better than a Chicago gathering, with the city's wonderful haunts, museums, restaurants, and fine memories of past conferences. The conference program covers nearly every…

  11. Context Sensitive Health Informatics

    DEFF Research Database (Denmark)

    Healthcare information technologies are now routinely deployed in a variety of healthcare contexts. These contexts differ widely, but the smooth integration of IT systems is crucial, so the design, implementation, and evaluation of safe, effective, efficient and easy to adopt health informatics...... involves careful consideration of both human and organizational factors. This book presents the proceedings of the Context Sensitive Health Informatics (CSHI) conference, held in Copenhagen, Denmark, in August 2013. The theme of this year’s conference is human and sociotechnical approaches. The Human......: patients and IT; usability test and evaluation; work tasks and related contexts; human factors and simulation; and context and systems design, and outline theories and models for studying contextual issues and insights related to how health information technologies can be better designed to accommodate...

  12. US green building conference - 1994

    Energy Technology Data Exchange (ETDEWEB)

    Fanney, A.H.; Whitter, K.M.; Traugott, A.E.; Simon, L.N. [eds.

    1994-12-31

    This report constitutes the proceedings of the Green Building Conference held in Gaithersburg, Maryland, February 16-17, 1994. The conference was co-sponsored by the National Institute of Standards and Technology (NIST) and the US Green Building Council (USGBC). Over 450 individuals attended the conference representing building product manufacturers, building owners and managers, environmental groups, utilities, contractors, builders, architects, engineers, and the local, state, and the federal governments. The conference provided an opportunity to acquire practical, useful information on green buildings, resources, and guidelines. Eighteen papers were presented at the conference. Separate abstracts and indexing were prepared for each paper for inclusion in the Energy Science and Technology Database.

  13. European Conference on Health Economics.

    Science.gov (United States)

    Malmivaara, Antti

    2010-12-01

    The biennial European Conference on Health Economics was held in Finland this year, at the Finlandia Hall in the centre of Helsinki. The European conferences rotate among European countries and fall between the biennial world congresses organized by the International Health Economics Association (iHEA). A record attendance of approximately 800 delegates from 50 countries around the world were present at the Helsinki conference. The theme of the conference was 'Connecting Health and Economics'. All major topics of health economics were covered in the sessions. For the first time, social care economics was included in the agenda of the European Conference as a session of its own.

  14. Silencing of MicroRNA-21 confers the sensitivity to tamoxifen and fulvestrant by enhancing autophagic cell death through inhibition of the PI3K-AKT-mTOR pathway in breast cancer cells.

    Science.gov (United States)

    Yu, Xinfeng; Li, Ruilian; Shi, Wenna; Jiang, Tao; Wang, Yufei; Li, Cong; Qu, Xianjun

    2016-02-01

    Tamoxifen (TAM) and fulvestrant (FUL) represent the major adjuvant therapy to estrogen receptor-alpha positive (ER(+)) breast cancer patients. However, endocrine resistance to TAM and FUL is a great impediment for successful treatment. We hypothesized that miR-21 might alter the sensitivity of breast cancer cells to TAM or FUL by regulating cell autophagy. Using the ER(+) breast cancer cells, we knockdown miR-21.by transfection with miR-21 inhibitor, then the cells were exposed to TAM or FUL and the percentages of apoptosis and autophagy were determined. Knockdown of miR-21 significantly increased the TAM or FUL-induced apoptosis in ER(+) breast cancer cells. Further, silencing of miR-21 in MCF-7 cells enhanced cell autophagy at both basal and TAM or FUL-induced level. The increase of autophagy in miR-21-knockdown MCF-7 cells was also indicated by increase of beclin-1, LC3-II and increased GFP-LC3 dots. Importantly, knockdown of miR-21 contributed to autophagic cell death, which is responsible for part of TAM induced cell death in miR-21 inhibitor-transfected cells. Further analysis suggested that miR-21 inhibitor enhance autophagic cell death through inhibition of PI3K-AKT-mTOR pathway. MiR-21 coordinated the function of autophagy and apoptosis by targeting Phosphatase and tensin homolog (PTEN) through inhibition of PI3K-AKT-mTOR pathway. In conclusion, silencing of miR-21 increased the sensitivity of ER(+) breast cancer cells to TAM or FUL by increasing autophagic cell death. Targeting autophagy-related miRNAs is a potential strategy for overcoming endocrine resistance to TAM and FUL.

  15. Energy Conferences and Symposia; (USA)

    Energy Technology Data Exchange (ETDEWEB)

    Osborne, J.H.; Simpson, W.F. Jr. (eds.)

    1991-01-01

    Energy Conferences and Symposia, a monthly publication, was instituted to keep scientists, engineers, managers, and related energy professionals abreast of meetings sponsored by the Department of Energy (DOE) and by other technical associations. Announcements cover conference, symposia, workshops, congresses, and other formal meetings pertaining to DOE programmatic interests. Complete meeting information, including title, sponsor, and contact, is presented in the main section, which is arranged alphabetically by subject area. Within a subject, citations are sorted by beginning data of the meeting. New listings are indicated by a bullet after the conference number and DOE-sponsored conferences are indicated by a star. Two indexes are provided for cross referencing conference information. The Chronological Index lists conference titles by dates and gives the subject area where complete information they may be found. The Location Index is alphabetically sorted by the city where the conference will be held.

  16. Computational Intelligence : International Joint Conference

    CERN Document Server

    Rosa, Agostinho; Cadenas, José; Dourado, António; Madani, Kurosh; Filipe, Joaquim

    2016-01-01

    The present book includes a set of selected extended papers from the sixth International Joint Conference on Computational Intelligence (IJCCI 2014), held in Rome, Italy, from 22 to 24 October 2014. The conference was composed by three co-located conferences:  The International Conference on Evolutionary Computation Theory and Applications (ECTA), the International Conference on Fuzzy Computation Theory and Applications (FCTA), and the International Conference on Neural Computation Theory and Applications (NCTA). Recent progresses in scientific developments and applications in these three areas are reported in this book. IJCCI received 210 submissions, from 51 countries, in all continents. After a double blind paper review performed by the Program Committee, 15% were accepted as full papers and thus selected for oral presentation. Additional papers were accepted as short papers and posters. A further selection was made after the Conference, based also on the assessment of presentation quality and audience in...

  17. Indico CONFERENCE tutorial

    CERN Document Server

    CERN. Geneva; Manzoni, Alex Marc

    2017-01-01

    This short tutorial explains how to create a CONFERENCE in indico and how to handle abstracts and registration forms, in detail: Timestamps: 1:01 - Programme  2:28 - Call for abstracts  11:50 - Abstract submission  13:41 - Abstract Review 15:41 - The Judge's Role 17:23 - Registration forms' creation 23:34 - Candidate participant's registration/application 25:54 - Customisation of Indico pages - Layout 28:08 - Customisation of Indico pages - Menus 29:47 - Configuring Event reminders and import into calendaring tools   See HERE a recent presentation by Pedro about the above steps in the life of an indico CONFERENCE event.

  18. Metabolic Engineering VII Conference

    Energy Technology Data Exchange (ETDEWEB)

    Kevin Korpics

    2012-12-04

    The aims of this Metabolic Engineering conference are to provide a forum for academic and industrial researchers in the field; to bring together the different scientific disciplines that contribute to the design, analysis and optimization of metabolic pathways; and to explore the role of Metabolic Engineering in the areas of health and sustainability. Presentations, both written and oral, panel discussions, and workshops will focus on both applications and techniques used for pathway engineering. Various applications including bioenergy, industrial chemicals and materials, drug targets, health, agriculture, and nutrition will be discussed. Workshops focused on technology development for mathematical and experimental techniques important for metabolic engineering applications will be held for more in depth discussion. This 2008 meeting will celebrate our conference tradition of high quality and relevance to both industrial and academic participants, with topics ranging from the frontiers of fundamental science to the practical aspects of metabolic engineering.

  19. 7th IAASS Conference

    CERN Document Server

    Rongier, Isabelle

    2015-01-01

    The 7th IAASS Conference, “Space Safety is No Accident” is an invitation to reflect and exchange information on a number of topics in space safety and sustainability of national and international interest. The conference is also a forum to promote mutual understanding, trust and the widest possible international cooperation in such matters. The once exclusive “club” of nations with autonomous sub-orbital and orbital space access capabilities is becoming crowded with fresh and ambitious new entrants. New commercial spaceports are starting operations and others are being built. In the manned spaceflight arena a commercial market is becoming a tangible reality with suborbital spaceflights and government use of commercial services for cargo and crew transportation to orbit. Besides the national ambitions in space, the international cooperation both civil and commercial is also gaining momentum. In the meantime robotic space exploration will accelerate and with it the need to internationally better regulat...

  20. XIX Edoardo Amaldi Conference

    CERN Document Server

    Abousahl, Said; Plastino, Wolfango

    2016-01-01

    This book, comprising contributions presented at the XIX Edoardo Amaldi Conference, examines important aspects of international cooperation aimed at enhancing nuclear safety, security, safeguards (the “3S”), and non-proliferation, thereby assisting in the development and maintenance of the verification regime and progress toward a nuclear weapon-free world. The Conference served as a forum where eminent scientists, diplomats, and policymakers could compare national perspectives and update international collaborations. The book opens by addressing the political, institutional, and legal dimensions of the 3S and non-proliferation; current challenges are discussed and attempts made to identify possible solutions and future improvements. Subsequent sections consider scientific developments that can contribute to increased effectiveness in the implementation of international regimes, particularly in critical areas, technology foresight, and the ongoing evaluation of current capabilities. The closing sections d...

  1. 2013 APPLEPIES Conference

    CERN Document Server

    2014-01-01

    This book provides a thorough overview of cutting-edge research on electronics applications relevant to industry, the environment, and society at large. A wide spectrum of application domains are covered, from automotive to space and from health to security, and special attention is devoted to the use of embedded devices and sensors for imaging, communication, and control. The book is based on the 2013 APPLEPIES Conference, held in Rome, which brought together researchers and stakeholders to consider the most significant current trends in the field of applied electronics and to debate visions for the future. Areas covered by the conference included information communication technology; biotechnology and biomedical imaging; space; secure, clean, and efficient energy; the environment; and smart, green, and integrated transport. As electronics technology continues to develop apace, constantly meeting previously unthinkable targets, further attention needs to be directed toward the electronics applications and th...

  2. Mississippi Climate & Hydrology Conference

    Energy Technology Data Exchange (ETDEWEB)

    Lawford, R.; Huang, J.

    2002-05-01

    The GEWEX Continental International Project (GCIP), which started in 1995 and completed in 2001, held its grand finale conference in New Orleans, LA in May 2002. Participants at this conference along with the scientists funded through the GCIP program are invited to contribute a paper to a special issue of Journal of Geophysical Research (JGR). This special JGR issue (called GCIP3) will serve as the final report on scientific research conducted by GCIP investigators. Papers are solicited on the following topical areas, but are not limited to, (1) water energy budget studies; (2) warm season precipitation; (3) predictability and prediction system; (4) coupled land-atmosphere models; (5) climate and water resources applications. The research areas cover observations, modeling, process studies and water resources applications.

  3. Moldova. Historic regional conference.

    Science.gov (United States)

    Moshin, V

    1995-05-01

    The Directorate of Maternal and Child Health and the Family Planning Association of Moldova organized a regional conference, which was held October 18-19, 1994, in Kishinev, Moldova, with the support of the United Nations Population Fund (UNFPA), the World Health Organization (WHO), and the International Planned Parenthood Federation (IPPF). The conference,"Problems of Family Planning in Eastern Europe," was attended by approximately 400 Moldovan delegates of governmental and nongovernmental organizations (NGOs), and by 25 delegates from Romania, Russia, Belarus, the Ukraine, and Georgia. The President of Moldova and the Ministry of Public Health of Moldova gave their approval. The main objectives of the conference were to inform the public about the recommendations of the ICPD, to analyze the status of women's reproductive health and family planning in Eastern Europe, and to find ways of implementing the ICPD Plan of Action. Major problems identified during the conference were: 1) the social and economic problems facing most families; 2) the high rate of morbidity and mortality; 3) the decrease in birth rate; 4) the increase in abortions; 5) the rising incidence of venereal disease; and 6) the absence of an effective family planning system. It was agreed that cooperation between governments and NGOs is essential in designing population programs for each country. The following goals were set: 1) to provide populations with sufficient contraceptives; 2) to actively promote family planning concepts through the mass media; 3) to train specialists and to open family planning offices and centers; 4) to introduce sex education in the curricula of Pedagogical Institutes; and 5) to create national and regional statistical and sociological databases on population issues.

  4. Genome sequencing conference II

    Energy Technology Data Exchange (ETDEWEB)

    1990-01-01

    Genome Sequencing Conference 2 was held September 30 to October 30, 1990. 26 speaker abstracts and 33 poster presentations were included in the program report. New and improved methods for DNA sequencing and genetic mapping were presented. Many of the papers were concerned with accuracy and speed of acquisition of data with computers and automation playing an increasing role. Individual papers have been processed separately for inclusion on the database.

  5. Conference on Logical Methods

    CERN Document Server

    Remmel, Jeffrey; Shore, Richard; Sweedler, Moss; Progress in Computer Science and Applied Logic

    1993-01-01

    The twenty-six papers in this volume reflect the wide and still expanding range of Anil Nerode's work. A conference on Logical Methods was held in honor of Nerode's sixtieth birthday (4 June 1992) at the Mathematical Sciences Institute, Cornell University, 1-3 June 1992. Some of the conference papers are here, but others are from students, co-workers and other colleagues. The intention of the conference was to look forward, and to see the directions currently being pursued, in the development of work by, or with, Nerode. Here is a brief summary of the contents of this book. We give a retrospective view of Nerode's work. A number of specific areas are readily discerned: recursive equivalence types, recursive algebra and model theory, the theory of Turing degrees and r.e. sets, polynomial-time computability and computer science. Nerode began with automata theory and has also taken a keen interest in the history of mathematics. All these areas are represented. The one area missing is Nerode's applied mathematica...

  6. 2004 Mutagenesis Gordon Conference

    Energy Technology Data Exchange (ETDEWEB)

    Dr. Sue Jinks-Robertson

    2005-09-16

    Mutations are genetic alterations that drive biological evolution and cause many, if not all, human diseases. Mutation originates via two distinct mechanisms: ''vertical'' variation is de novo change of one or few bases, whereas ''horizontal'' variation occurs by genetic recombination, which creates new mosaics of pre-existing sequences. The Mutagenesis Conference has traditionally focused on the generation of mutagenic intermediates during normal DNA synthesis or in response to environmental insults, as well as the diverse repair mechanisms that prevent the fixation of such intermediates as permanent mutations. While the 2004 Conference will continue to focus on the molecular mechanisms of mutagenesis, there will be increased emphasis on the biological consequences of mutations, both in terms of evolutionary processes and in terms of human disease. The meeting will open with two historical accounts of mutation research that recapitulate the intellectual framework of this field and thereby place the current research paradigms into perspective. The two introductory keynote lectures will be followed by sessions on: (1) mutagenic systems, (2) hypermutable sequences, (3) mechanisms of mutation, (4) mutation avoidance systems, (5) mutation in human hereditary and infectious diseases, (6) mutation rates in evolution and genotype-phenotype relationships, (7) ecology, mutagenesis and the modeling of evolution and (8) genetic diversity of the human population and models for human mutagenesis. The Conference will end with a synthesis of the meeting as the keynote closing lecture.

  7. PREFACE: Wake Conference 2015

    Science.gov (United States)

    Barney, Andrew; Nørkær Sørensen, Jens; Ivanell, Stefan

    2015-06-01

    The 44 papers in this volume constitute the proceedings of the 2015 Wake Conference, held in Visby on the island of Gotland in Sweden. It is the fourth time this conference has been held. The Wake Conference series started in Visby, where it was held in 2009 and 2011. In 2013 it took place in Copenhagen where it was combined with the International Conference on Offshore Wind Energy and Ocean Energy. In 2015 it is back where it started in Visby, where it takes place at Uppsala University Campus Gotland, June 9th-11th. The global yearly production of electrical energy by wind turbines has grown tremendously in the past decade and it now comprises more than 3% of the global electrical power consumption. Today the wind power industry has a global annual turnover of more than 50 billion USD and an annual average growth rate of more than 20%. State-of-the-art wind turbines have rotor diameters of up to 150 m and 8 MW installed capacity. These turbines are often placed in large wind farms that have a total production capacity corresponding to that of a nuclear power plant. In order to make a substantial impact on one of the most significant challenges of our time, global warming, the industry's growth has to continue for a decade or two yet. This in turn requires research into the physics of wind turbine wakes and wind farms. Modern wind turbines are today clustered in wind farms in which the turbines are fully or partially influenced by the wake of upstream turbines. As a consequence, the wake behind the wind turbines has a lower mean wind speed and an increased turbulence level, as compared to the undisturbed flow outside the farm. Hence, wake interaction results in decreased total production of power, caused by lower kinetic energy in the wind, and an increase in the turbulence intensity. Therefore, understanding the physical nature of the vortices and their dynamics in the wake of a turbine is important for the optimal design of a wind farm. This conference is aimed

  8. Context Sensitive Health Informatics

    DEFF Research Database (Denmark)

    2013-01-01

    Context is a key consideration when designing and evaluating health information technology (HIT) and cannot be overstated. Unintended consequences are common post HIT implementation and even well designed technology may not achieve desired outcomes because of contextual issues. While context should...... be considered in the design and evaluation of health information systems (HISs) there is a shortcoming of empirical research on contextual aspects of HIT. This conference integrates the sociotechnical and Human-Centered-Design (HCD) approaches and showcases current research on context sensitive health...

  9. Conference Report: The First ATLAS.ti User Conference

    OpenAIRE

    Evers, Jeanine C.; Silver, Christina

    2014-01-01

    This report on the First ATLAS.ti User Conference shares our impressions and experiences as longstanding ATLAS.ti users and trainers about the First ATLAS.ti User Conference in Berlin 2013. The origins, conceptual principles and development of the program are outlined, the conference themes discussed and experiences shared. Finally, the future of the program is discussed.URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs1401197

  10. Computational Intelligence : International Joint Conference

    CERN Document Server

    Dourado, António; Rosa, Agostinho; Filipe, Joaquim; Kacprzyk, Janusz

    2016-01-01

    The present book includes a set of selected extended papers from the fifth International Joint Conference on Computational Intelligence (IJCCI 2013), held in Vilamoura, Algarve, Portugal, from 20 to 22 September 2013. The conference was composed by three co-located conferences:  The International Conference on Evolutionary Computation Theory and Applications (ECTA), the International Conference on Fuzzy Computation Theory and Applications (FCTA), and the International Conference on Neural Computation Theory and Applications (NCTA). Recent progresses in scientific developments and applications in these three areas are reported in this book. IJCCI received 111 submissions, from 30 countries, in all continents. After a double blind paper review performed by the Program Committee, only 24 submissions were accepted as full papers and thus selected for oral presentation, leading to a full paper acceptance ratio of 22%. Additional papers were accepted as short papers and posters. A further selection was made after ...

  11. Joint US/German Conference

    CERN Document Server

    Gulledge, Thomas; Jones, Albert

    1993-01-01

    This proceedings volume contains selected and refereed contributions that were presented at the conference on "Recent Developments and New Perspectives of Operations Research in the Area of Production Planning and Control" in Hagen/Germany, 25. - 26. June 1992. This conference was organized with the cooperation of the FernuniversiHit Hagen and was jointly hosted by the "Deutsche Gesellschaft fur Operations Research (DGOR)" and the "Manufacturing Special Interest Group of the Operations Research Society of America (ORSA-SIGMA)". For the organization of the conference we received generous financial support from the sponsors listed at the end of this volume. We wish to express our appreciation to all supporters for their contributions. This conference was the successor of the JOInt ORSA/DGOR-conference in Gaithersburg/Maryland, USA, on the 30. and 31. July 1991. Both OR-societies committed themselves in 1989 to host joint conferences on special topics of interest from the field of operations research. This goal ...

  12. 14th International Cryocooler Conference

    CERN Document Server

    Ross, Ronald G

    2007-01-01

    This is the 14th volume in the conference series. Over the years the International Cryocoolers Conference has become the preeminent worldwide conference for the presentation of the latest developments and test experiences with cryocoolers. The typical applications of this technology include cooling space and terrestrial infrared focal plane arrays, space x-ray detectors, medical applications, and a growing number of high-temperature superconductor applications.

  13. 15th International Cryocooler Conference

    CERN Document Server

    Ross, Ronald G

    2009-01-01

    This is the 15th volume in the conference series. Over the years the International Cryocooler Conference has become the preeminent worldwide conference for the presentation of the latest developments and test experiences with cryocoolers. The typical applications of this technology include cooling space and terrestrial infrared focal plane arrays, space x-ray detectors, medical applications, and a growing number of high-temperature superconductor applications.

  14. International conference on string theory

    CERN Document Server

    2017-01-01

    The Strings 2017 conference is part of the "Strings" series of annual conferences, that bring the entire string theory community together. It will include reviews of major developments in the field, and specialized talks on specific topics. There will also be several public lectures given by conference participants, a pre-Strings school at the Technion, and a post-Strings workshop at the Weizmann Institute.

  15. 17th International Cryocooler Conference

    CERN Document Server

    Ross, Ronald G

    2012-01-01

    Cryocoolers 17 archives developments and performance measurements in the field of cryocoolers based on the contributions of leading international experts at the 17th International Cryocooler Conference that was held in Los Angeles, California, on July 9-12, 2012. The program of this conference consisted of 94 papers; of these, 71 are published here. Over the years the International Cryocoolers Conference has become the preeminent worldwide conference for the presentation of the latest developments and test experiences with cryocoolers. The typical applications of this technology include cooling space and terrestrial infrared focal plane arrays, space x-ray detectors, medical applications, and a growing number of high-temperature superconductor applications.

  16. 16th International Cryocooler Conference

    CERN Document Server

    Ross, Ronald G

    2011-01-01

    Cryocoolers 16 archives developments and performance measurements in the field of cryocoolers based on the contributions of leading international experts at the 16th International Cryocooler Conference that was held in Atlanta, Georgia, on May 17-20, 2010. The program of this conference consisted of 116 papers; of these, 89 are published here. Over the years the International Cryocoolers Conference has become the preeminent worldwide conference for the presentation of the latest developments and test experiences with cryocoolers. The typical applications of this technology include cooling space and terrestrial infrared focal plane arrays, space x-ray detectors, medical applications, and a growing number of high-temperature superconductor applications.

  17. VIENNA INTERNATIONAL CONFERENCES - FEBRUARY 2012

    Directory of Open Access Journals (Sweden)

    VIPCA

    2012-01-01

    Full Text Available VIPCA, the Vienna International Plant Conference Association, a non-commercial and non-profit organization consisting of academy and industry scientists from across the world, founded to support the international plant and agricultural science community by initiating several major conferences every year in Vienna. The Vienna International Plant Conference Association provides, on an international level, an opportunity for researchers to explore ideas for future innovations and collaborations. The VIPCA is organizing 4 international conferences in 2012. Meetings will be held in Vienna, the capital of Austria. Topics will cover the hottest issues in current plant science. For more details please download the full text file.

  18. 4th International Cryocoolers Conference

    CERN Document Server

    Patton, George; Knox, Margaret

    1987-01-01

    The Cryocoolers 4 proceedings archives the contributions of leading international experts at the 4th International Cryocooler Conference that was held in Easton, Maryland on September 25-26, 1986. About 170 people attended the conference representing 11 countries, 14 universities, 21 government laboratories and 60 industrial companies. Thirty-one papers were presented describing advancements and applications of cryocoolers in the temperature range below 80K. This year's conference was sponsored by the David Taylor Naval Ship Research and Development Center of Annapolis, Maryland, and the conference proceedings reproduced here was published by them.

  19. Dynamical Systems Conference

    CERN Document Server

    Gils, S; Hoveijn, I; Takens, F; Nonlinear Dynamical Systems and Chaos

    1996-01-01

    Symmetries in dynamical systems, "KAM theory and other perturbation theories", "Infinite dimensional systems", "Time series analysis" and "Numerical continuation and bifurcation analysis" were the main topics of the December 1995 Dynamical Systems Conference held in Groningen in honour of Johann Bernoulli. They now form the core of this work which seeks to present the state of the art in various branches of the theory of dynamical systems. A number of articles have a survey character whereas others deal with recent results in current research. It contains interesting material for all members of the dynamical systems community, ranging from geometric and analytic aspects from a mathematical point of view to applications in various sciences.

  20. 2nd UNet conference

    CERN Document Server

    Menasche, Daniel; Sabir, Essaïd; Pellegrini, Francesco; Benjillali, Mustapha

    2017-01-01

    This volume offers the proceedings of the 2nd UNet conference, held in Casablanca May 30 - June 1, 2016. It presents new trends and findings in hot topics related to ubiquitous computing/networking, covered in three tracks and three special sessions: Main Track 1: Context-Awareness and Autonomy Paradigms Track Main Track 2: Mobile Edge Networking and Virtualization Track Main Track 3: Enablers, Challenges and Applications Special Session 1: Smart Cities and Urban Informatics for Sustainable Development Special Session 2: Unmanned Aerial Vehicles From Theory to Applications Special Session 3: From Data to Knowledge: Big Data applications and solutions.

  1. 76 FR 57746 - Conference on the International Conference on Harmonisation Q10 Pharmaceutical Quality System: A...

    Science.gov (United States)

    2011-09-16

    ... HUMAN SERVICES Food and Drug Administration Conference on the International Conference on Harmonisation... teaching the principles of the International Conference on Harmonisation of Technical Requirements for... Systems and Processes for Pharmaceutical Manufacturing; Public Conference AGENCY: Food and...

  2. CONFERENCE REPORT: Code4Lib 2010

    Directory of Open Access Journals (Sweden)

    Birong Ho

    2010-03-01

    Full Text Available Conference reports from the 5th Code4Lib Conference, held in Asheville, NC, from February 22 to 25, 2010. The Code4Lib conference is a collective volunteer effort of the Code4Lib community of library technologists. Included are three brief reports on the conference from the recipients of conference scholarships.

  3. CONFERENCE REPORT: Code4Lib 2009

    Directory of Open Access Journals (Sweden)

    Lauren Ko

    2009-03-01

    Full Text Available Conference reports from the 4th Code4Lib conference, held in Providence, RI from February 23 to 26, 2009. The Code4Lib conference is a collective volunteer effort of the informal Code4Lib community of library technologists. Included are four brief reports on the conference from the recipients of conference scholarships.

  4. 20. AINSE plasma science and technology conference. Conference handbook

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-31

    The 20th AINSE plasma science and technology conference was held at Flinders University of South Australia on 13-14 February 1995. Topics under discussion included plasma physics studies, current status of rotamak devices, plasma processing and material studies. The handbook contains the conference program, 54 abstracts and a list of participants.

  5. (Tribology conferences and forums)

    Energy Technology Data Exchange (ETDEWEB)

    Yust, C.S.

    1990-11-30

    The principal meeting attended during this trip was the Japan International Tribology Conference Nagoya 1990. The conference encompassed a wide range of topics, including the tribology of ceramics, the tribology in high-performance automobiles, and many aspects of lubrication technology. Associated forums were also held on the tribology of advanced ceramics, on solid lubrication, and on automotive lubricants. Presentations made during the latter forum discussed anticipated trends in engine development and anticipated improvements in lubricants required for the next generation of engines. In addition to meetings, site visits were made to five industrial organizations to discuss ceramic tribology. Nippon Steel Corporation and Toshiba Corporation are both very active in the ceramic area, Nippon Steel from their interest in research on new materials and Toshiba from both an interest in new materials and in support of their work in electronic devices. Two engine manufacturers were also visited, Toyota Motor Corporation, and Nissan Motor Co., Ltd. These companies were somewhat reserved in their discussion of progress in the utilization of ceramics in automobile engines.

  6. Annual conference SAEE 2008

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    Technical and economic challenges of a 1 t CO{sub 2} society was the topic addressed by the 2008 annual conference of the Swiss Association for Energy Economics. One tonne of carbon dioxide per head and year as a long-term energy strategy is the theme of a presentation made by professor Konstantin Boulouchos from the Swiss Federal Institute of Technology ETH in Zurich, Switzerland. Professor Dr. Rainhard Madlener from the Institute for Future Energy Consumer Needs and Behaviour in Aachen, Germany, took a look at the one-ton CO{sub 2} vision as a focus for technical development. Professor Thomas F. Rutherford from the ETH presented an economic analysis of one-ton CO{sub 2} scenarios. Eduard Schumacher, former Chairman of the Board at the IWB utility in Basel, Switzerland, presented examples of how energy policy can be implemented, using the IWB's activities as an example. Hansruedi Kunz, Head of the Energy Department in the Building Department of the Canton of Zurich discussed the chances offered and the problems posed by the implementation of measures that are to lead to the meeting of energy visions for the year 2050. A podium and discussion session completed the conference

  7. History of NAMES Conferences

    Science.gov (United States)

    Filippov, Lev

    2013-03-01

    -Russian International Centre was demonstrated. By the high standards of the reports presented, as well as by its overall organization, the second Seminar met the standards of an international conference. Reviews of state-of-the-art developments in materials science were given by leading scientists from Moscow and from the Lorraine region. The three days of the seminar were structured into four main themes: Functional Materials Coatings, Films and Surface Engineering Nanomaterials and Nanotechnologies The Environment and three Round Table discussions: Defining practical means of carrying out Franco-Russian collaborations in technology transfer and innovation Materials science ARCUS: Lorraine-Russian collaboration in materials science and the environment 32 oral and 25 poster presentations within four sections were given by a total of 110 participants. NAMES 2007, the 3rd Franco-Russian Seminar on New Achievements in Materials and Environmental Sciences, took place in Metz, France on 7-9 November 2007. The conference highlights fundamentals and development of the five main themes connected to the Lorraine-Russia ARCUS project with possible extension to other topics. The five main subjects included in the ARCUS project are: Bulk-surface-interface material sciences Nanomaterials and nanotechnologies Environment and natural resources Plasma physics—ITER project Vibrational dynamics The first, second and third NAMES conferences were financially supported by the following organizations: Ambassade de France à Moscou Communauté Urbaine du Grand Nancy Région Lorraine Conseil Général de Meurthe et Moselle Institut National Polytechnique de Lorraine Université de Metz Université Henry Poincaré CNRS ANVAR Federal Agency on Science and Innovations of the Ministry of Education and Science of the Russian Federation Moscow Committee on Science and Technologies Moscow Institute of Steel and Alloys (Technological University) The 4th conference is supported by the Ministry of Foreign Affairs of

  8. Calendar of Conferences

    Science.gov (United States)

    1996-08-01

    8 - 18 August 1996 International Summer School on Plasma Physics and Technology La Jolla, CA, USA Contact: Mr V Stefan, Institute for Advanced Physics Studies, PO Box 2964, La Jolla, CA 92038, USA. Tel +1-619-456-5737. 26 - 30 August 1996 Joint Varenna - Lausanne International Workshop on Theory of Fusion Plasmas Villa Monastero, Varenna, Italy Contact: Centro di Cultura Villa Monastero, 1 Piazza Venini, 22050 Varenna (Lecco), Italy. Tel +39-341-831261, Fax +39-341-831281. Application and abstract deadline: 15 June 1996. 2 - 5 September 1996 EU - US Workshop on Transport in Fusion Plasmas Villa Monastero, Varenna, Italy Further information: G Gorini, ISPP, 16 Via Celoria, I-20133 Milano, Italy. Tel +39-2-2392637, Fax +39-2-2392205, E-mail ggorini@mi.infn.it. Administrative contact: Centro di Cultura Villa Monastero, 1 Piazza Venini, 22050 Varenna (Lecco), Italy. Tel +39-341-831261, Fax +39-341-831281. Application and abstract deadline: 15 June 1996. 9 - 13 September 1996 International Conference on Plasma Physics Nagoya, Japan Contact: Conference Secretariat, c/o Prof. Hiromu Momota, National Institute for Fusion Science, Nagoya 464-01, Japan. Tel +81-52-789-4260, Fax +81-52-789-1037, E-mail icpp96@nifs.ac.jp. Abstract deadline: 31 March 1996. 16 - 20 September 1996 19th Symposium on Fusion Technology Lisbon, Portugal Contact: Professor Carlos Varandas, Centro de Fusão Nuclear, 1096 Lisboa Codex, Portugal. Fax +351-1-8417819, E-mail cvarandas@cfn.ist.utl.pt. General information will be available via WWW with URL http://www.cfn.ist.utl.pt. 25 - 29 September 1996 Summer University of Plasma Physics Garching, Germany Contact: Ms Ch Stahlberg, Max-Planck-Institut für PlasmaPhysik, Boltzmannstr 2, D-85748 Garching, Germany. Tel +49-89-3299-2232, Fax +49-89-3299-1001. 11 - 15 November 1996 38th Annual Meeting of the Division of Plasma Physics, APS Denver, CO, USA Contact: Dr Richard Hazeltine, University of Texas, Institute for Fusion Studies, RLM 11.314, Austin, TX

  9. Cellular basis for the species differences in sensitivity to cardiac glycosides (digitalis).

    Science.gov (United States)

    Gupta, R S; Chopra, A; Stetsko, D K

    1986-05-01

    The relative toxicity of numerous cardiotonic steroids (viz. ouabain, digitoxin, digoxin, convallatoxin, SC4453, bufalin, gitaloxin, digoxigenin, actodigin, oleandrin, digitoxigenin, gitoxin, strophanthidin, gitoxigenin, lanatosides A, B and C, alpha- and beta-acetyl digoxin, alpha- and beta-methyl digoxin) and related compounds towards a number of independent cell lines established from human, monkey, mouse, Syrian hamster, and Chinese hamster have been determined. All cardiac glycosides and their genins, as well as the cardiotonic alkaloid cassaine, exhibited greater than 100-fold higher toxicity towards cultured human and monkey cells in comparison to the cell lines of mouse, Syrian hamster, and Chinese hamster origins. These differences are species-related as all cell lines (both normal as well as transformed) from any one species, as well as cells from the closely related species (e.g., man and monkey or mouse, Chinese hamster, and Syrian hamster), showed similar sensitivity towards these drugs. The failure to see any significant differences in cellular toxicity for a larger number of other compounds which either bear limited structural resemblance to cardiac glycosides (viz. estradiol 17-beta-acetate, testosterone propionate, 21-acetoxy pregnenolone, beta-estradiol, digitonin, tigogenin, and tomatine) or interact with the Na+/K+ ATPase in a different manner (viz. veratridine, sanguinarine nitrate, penicillic acid, vanadium pentoxide, harmaline-HCI,5,5'-diphenyl hydantoin, quindonium bromide, and methyl quinolizinum bromide) provides strong evidence that the observed species-related differences are highly specific for cardiotonic steroids. Studies on the binding of [3H]ouabain show that, in comparison to human and monkey cell lines, no significant binding of the drug is observed in cells derived from the resistant species (i.e., mouse and Chinese hamster). The Na+/K+ ATPase from cells of the resistant species is inhibited at much higher concentrations of

  10. SPECIAL REPORT: Creating Conference Video

    Directory of Open Access Journals (Sweden)

    Noel F. Peden

    2008-12-01

    Full Text Available Capturing video at a conference is easy. Doing it so the product is useful is another matter. Many subtle problems come into play so that video and audio obtained can be used to create a final product. This article discusses what the author learned in the two years of shooting and editing video for Code4Lib conference.

  11. Building Bridges through Scientific Conferences

    DEFF Research Database (Denmark)

    Zierath, Juleen R

    2016-01-01

    Getting together to exchange ideas, forge collaborations, and disseminate knowledge is a long-standing tradition of scientific communities. How conferences are serving the community, what their current challenges are, and what is in store for the future of conferences are the topics covered...

  12. The Writing Conference as Performance.

    Science.gov (United States)

    Newkirk, Thomas

    1995-01-01

    Provides an overview of the conversational roles taken on by students and teachers during college-level writing conferences. Uses the performative theory of Erving Goffman to analyze these role patterns. Illuminates the specific performative demands presented by writing conferences on both students and teachers. (HB)

  13. Vague Language in Conference Abstracts

    Science.gov (United States)

    Cutting, Joan

    2012-01-01

    This study examined abstracts for a British Association for Applied Linguistics conference and a Sociolinguistics Symposium, to define the genre of conference abstracts in terms of vague language, specifically universal general nouns (e.g. people) and research general nouns (e.g. results), and to discover if the language used reflected the level…

  14. Solar energy conference, final report

    Energy Technology Data Exchange (ETDEWEB)

    None

    1977-05-24

    The conference attendance, publicity and press coverage, brochure mailing, presentations, displays, exhibitors, management seminar checklist, and seminar evaluation by attendees are presented. Also included are the proposal for funding of the conference, the list of attendees, keynote speeches, agenda, and feedback questionnaire. (MHR)

  15. SLA at 100: Conference Preview

    Science.gov (United States)

    Blumenstein, Lynn

    2009-01-01

    When School Library Association (SLA) convenes its annual conference in Washington, DC, June 14-17, 2009, the association will be celebrating its 100th birthday. This occasion allows for grand gestures--the SLA Salutes! Awards and Leadership Reception will be held in the Library of Congress's Great Hall. The conference also draws upon Washington…

  16. 4th European Turbulence Conference

    CERN Document Server

    1993-01-01

    The European Turbulence Conferences have been organized under the auspices of the European Mechanics Committee (Euromech) to provide a forum for discussion and exchange of recent and new results in the field of turbulence. The first conference was organized in Lyon in 1986 with 152 participants. The second and third conferences were held in Berlin (1988) and Stockholm (1990) with 165 and 172 participants respectively. The fourth was organized in Delft from 30 June to 3 July 1992 by the J.M. Burgers Centre. There were 214 participants from 22 countries. This steadily growing number of participants demonstrates both the success and need for this type of conference. The main topics of the Fourth European Turbulence Conference were: Dynamical Systems and Transition; Statistical Physics and Turbulence; Experiments and Novel Experimental Techniques; Particles and Bubbles in Turbulence; Simulation Methods; Coherent Structures; Turbulence Modelling and Compressibility Effects. In addition a special session was held o...

  17. Eastern Pacific Ocean Conference

    Science.gov (United States)

    The promotion of interaction among investigators of all oceanographic disciplines studying the eastern Pacific Ocean was the goal of the 1990 Eastern Pacific Ocean Conference (EPOC), held October 17-19 on the snow-covered slopes of Mt. Hood, Oreg. Thirty oceanographers representing all disciplines attended.Dick Barber, Duke University Marine Lab, Beaufort, N.C., chaired a session on the eastern equatorial Pacific Ocean, emphasizing issues related to biological activity. Steve Ramp of the Naval Postgraduate School in Montery, Calif., chaired a session on recent results from northern and central California experiments. On October 19, following an early morning earthquake, a business meeting and discussions regarding a collaboration in future experiments were held.

  18. Conference on Multibody Dynamics

    CERN Document Server

    Multibody Dynamics : Computational Methods and Applications

    2014-01-01

    By having its origin in analytical and continuum mechanics, as well as in computer science and applied mathematics, multibody dynamics provides a basis for analysis and virtual prototyping of innovative applications in many fields of contemporary engineering. With the utilization of computational models and algorithms that classically belonged to different fields of applied science, multibody dynamics delivers reliable simulation platforms for diverse highly-developed industrial products such as vehicle and railway systems, aeronautical and space vehicles, robotic manipulators, smart structures, biomechanical applications and nano-technologies. The chapters of this volume are based on the revised and extended versions of the selected scientific papers from amongst 255 original contributions that have been accepted to be presented within the program of the distinguished international ECCOMAS conference. It reflects state-of-the-art in the advances of multibody dynamics, providing excellent insight in the recen...

  19. ICPAQGP 2001: Conference summary

    Indian Academy of Sciences (India)

    Reinhard Stock

    2003-05-01

    I review recent progress in ultrarelativistic nucleus–nucleus collisions, and the connection of this field to modern QCD theory of deconfinement and/or chiral symmetry restoration. The talks at this Conference have shown a convergence of data and theory as far as the CERN SPS investigations at $\\sqrt{s}=17$ GeV are concerned; the parton–hadron phase boundary seems now located at = 170 ± 10 MeV. New data from RHIC and direct photon production results from CERN have been shown that point out the field’s future direction: analysis of partonic matter at > 200 MeV. Astrophysics analysis was shown to be linked crucially to further theoretical progress with non-perturbative QCD.

  20. COMPDYN 2011 Conference

    CERN Document Server

    Fragiadakis, Michalis; Plevris, Vagelis; Computational Methods in Earthquake Engineering v.2

    2013-01-01

    This book provides an insight on advanced methods and concepts for the design and analysis of structures against earthquake loading. This second volume is a collection of 28 chapters written by leading experts in the field of structural analysis and earthquake engineering. Emphasis is given on current state-of-the-art methods and concepts in computing methods and their application in engineering practice. The book content is suitable for both practicing engineers and academics, covering a wide variety of topics in an effort to assist the timely dissemination of research findings for the mitigation of seismic risk. Due to the devastating socioeconomic consequences of seismic events, the topic is of great scientific interest and is expected to be of valuable help to scientists and engineers. The chapters of this volume are extended versions of selected papers presented at the COMPDYN 2011 conference, held in the island of Corfu, Greece, under the auspices of the European Community on Computational Methods in Ap...

  1. International Conference UMC 2013

    CERN Document Server

    Hübner, Wolfgang; Rasing, Theo; Chantrell, Roy

    2015-01-01

    This volume on Ultrafast Magnetism is a collection of articles presented at the international “Ultrafast Magnetization Conference” held at the Congress Center in Strasbourg, France, from October 28th to November 1st, 2013. This first conference, which is intended to be held every two years, received a wonderful attendance and gathered scientists from 27 countries in the field of Femtomagnetism, encompassing many theoretical and experimental research subjects related to the spins dynamics in bulk or nanostructured materials. The participants appreciated this unique opportunity for discussing new ideas and debating on various physical interpretations of the reported phenomena. The format of a single session with many oral contributions as well as extensive time for poster presentations allowed researchers to have a detailed overview of the field. Importantly, one could sense that, in addition to studying fundamental magnetic phenomena, ultrafast magnetism has entered in a phase where applied physics and eng...

  2. The Haltenbanken Conference; Haltenbankenkonferansen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-01

    The publication includes a collection of papers from a conference on the development of Haltenbanken on the Norwegian continental shelf. Topics are as follow: Future perspectives for the Norwegian oil and gas activity; Haltenbanken - Saga`s new basic area; The Tyrihans field; The Njord field, from development to operation in 1997. The Ormen Lange field, feasibility of new technical solutions on great depths of water; The Norne field, experience from the commissioning of a production vessel; construction of hulls in Norway, self-reliance for the Norwegian industry; Experience of contractual concepts of maintenance and modification in Statoil; the Heidrun field, experience from the first working year and further challenges; Tjeldbergodden, small-scale utilization of gas; development of Tjeldbergodden as an industrial location. None of the papers are prepared. 38 figs., 2 tabs.

  3. International Logistics Science Conference

    CERN Document Server

    Hompel, Michael; Meier, J

    2014-01-01

    The importance of logistics in all its variations is still increasing. New technologies emerge, new planning methods and algorithms are developed, only to face a market with a growing complexity and the need of weighting monetary costs against ecological impact. Mastering these challenges requires a scientific viewpoint on logistics, but always with applications in mind. This volume presents up-to-date logistics research in all its diversity and interconnectedness. It grew out of the “International Logistics Science Conference” (ILSC) held in Dortmund in September 2013, bringing together leading scientists and young academics from nine different countries. The conference was jointly organized by the “Efficiency Cluster Logistics” and the “Fraunhofer Institute for Material Flow and Logistics”. The Program Committee used a double blind review process to choose the 12 strongest contributions, which were then grouped in four areas: - Sustainability logistics, including electric mobility, smart inform...

  4. Istanbul Bridge Conference 2014

    CERN Document Server

    Gülkan, Polat; Mahmoud, Khaled

    2016-01-01

      The book includes peer-reviewed contributions selected from presentations given at the Istanbul Bridge Conference 2014, held from August 11 – 13 in Istanbul, Turkey. It reports on the current challenges in bridge engineering faced by professionals around the globe, giving a special emphasis to recently developed techniques, innovations and opportunities. The book covers key topics in the field, including modeling and analysis methods; construction and erection techniques; design for extreme events and condition assessment and structural health monitoring. There is a balanced presentation of theory, research and practice. This book, which provides the readers with a comprehensive and timely reference guide on current practices in bridge engineering, is intended for professionals, academic researchers and students alike.

  5. The Geometry Conference

    CERN Document Server

    Bárány, Imre; Vilcu, Costin

    2016-01-01

    This volume presents easy-to-understand yet surprising properties obtained using topological, geometric and graph theoretic tools in the areas covered by the Geometry Conference that took place in Mulhouse, France from September 7–11, 2014 in honour of Tudor Zamfirescu on the occasion of his 70th anniversary. The contributions address subjects in convexity and discrete geometry, in distance geometry or with geometrical flavor in combinatorics, graph theory or non-linear analysis. Written by top experts, these papers highlight the close connections between these fields, as well as ties to other domains of geometry and their reciprocal influence. They offer an overview on recent developments in geometry and its border with discrete mathematics, and provide answers to several open questions. The volume addresses a large audience in mathematics, including researchers and graduate students interested in geometry and geometrical problems.

  6. Nostradamus conference 2013

    CERN Document Server

    Chen, Guanrong; Rössler, Otto; Snasel, Vaclav; Abraham, Ajith; Nostradamus 2013: Prediction, Modeling and Analysis of Complex Systems

    2013-01-01

    Prediction of behavior of the dynamical systems, analysis and modeling of its structure is vitally important problem in engineering, economy and science today. Examples of such systems can be seen in the world around us and of course in almost every scientific discipline including such “exotic” domains like the earth’s atmosphere, turbulent fluids, economies (exchange rate and stock markets), population growth, physics (control of plasma), information flow in social networks and its dynamics, chemistry and complex networks. To understand such dynamics and to use it in research or industrial applications, it is important to create its models. For this purpose there is rich spectra of methods, from classical like ARMA models or Box Jenkins method to such modern ones like evolutionary computation, neural networks, fuzzy logic, fractal geometry, deterministic chaos and more. This proceeding book is a collection of the accepted papers to conference Nostradamus that has been held in Ostrava, Czech Republic. P...

  7. Effects of hydrogen sulfide on arrhythmias induced by ouabain and L-type calcium channel of myocardial cell%硫化氢对心室肌细胞触发活动及L型钙通道的影响

    Institute of Scientific and Technical Information of China (English)

    刘慧霞

    2015-01-01

    目的:研究硫化氢(H2 S)对哇巴因诱发的触发性心律失常及 L 型钙通道的作用,探讨硫化氢抗心律失常的机制。方法运用全细胞膜片钳技术,记录 L 型钙通道电流,观察硫化氢对单个心室肌细胞 L 型钙通道各参数的影响。结果 H2 S 抑制哇巴因诱发的迟后去极化(DAD)及触发活动(TA),50,100和200μmol/ L 硫氢化钠(H2 S 的供体)使 ICa - L的峰值降低,浓度依赖性的使 I - V 曲线上移,峰值由(-13.9±1.4)pA/ pF 分别至(-10.7±1.4)pA/ pF(n =6,P <0.05)、(-7.4±2.5)pA/ pF(P <0.01)和(-5.3±1.1)pA/ pF(P <0.01),H2 S 使稳态激活曲线右移,而不影响稳态失活曲线。结论 H2 S 通过对 L 型钙通道的抑制而发挥抗心律失常作用。%Objective:To investigate effects of hydrogen sulfide(H2 S)on arrhythmias induced by ouabain and L-type calcium channel of myocardial cell. Methods:The whole-cell patch clamp technique was applied to record the current of L-type calcium channel and observe the effects on arrhythmias induced by ouabain and L-type calcium channel of single ar-rhythmic ventricular cell. Results:H2 S showed protective function of inhibiting the delayed afterdepolarization(DAD)and triggerd activity(TA)induced by ouabain. The peak current of Ica - L was decreased from( - 13. 9 ± 1. 4)pA/ pF to( -10. 7 ± 1. 4)pA/ pF(n = 5,P < 0. 05),( - 7. 4 ± 2. 5)pA/ pF(P < 0. 01)and( - 5. 3 ± 1. 1)pA/ pF(P < 0. 01)by 50, 100,200μmol sodium hydrosulfide(H2 S donor)respectively,and I - V curve was shifted upward in a concentration-de-pendent manner. The steady state activation curve was shifted to the right by H2 S while no effect on inactivation curve was shown. Conclusion:H2 S shows anti-arrhythmic function through its effects on L-type calcium channel.

  8. Conference Report: 5th Annual Georgia Conference on Information Literacy

    Directory of Open Access Journals (Sweden)

    Rebecca Ziegler

    2009-11-01

    Full Text Available The 5th annual Georgia Conference on Information Literacy took place in Savannah, Georgia on October 3-4, 2008. Since its inception, this conference has drawn participants from across the United States and even a few from abroad. Jointly sponsored by the Zach S. Henderson Library, the Department of Writing and Linguistics, the College of Education, and the Center for Continuing Education at Georgia Southern University, the conference offers both theoretical and practical discussions of the complex issues involved in teaching students how to find, interpret and use information in emerging electronic technologies against the backdrop of one of America’s loveliest cities.

  9. The 9. European nuclear conference; La 9. conference nucleaire europeenne

    Energy Technology Data Exchange (ETDEWEB)

    Maurel, V.; Lewis, D.; Smirnov, V.P.; Gutierrez, J.E.; Paulin, Ph.; Markov, D.V.; Smirnov, A.V.; Polenok, V.S.; Horhoianu, G.; Olteanu, G.; Van der Schaaf, B.; Gavillet, D.; Lapena, J.; Ohms, C.; Roth, A.; Van Dyck, St.; Mardon, J.P.; Thomas, A.; Cipiere, M.F.; Faidy, C.; Hedin, F.; Delnondedieu, M.; Chassignole, B.; Doudet, L.; Dupond, O.; Kang, K.; Park, K.; Kim, K.; Ha, J.; Hoon-Seok, Jung; Yong-koo, Lee; Kwang-Ho, Kim; Seungwoo, Paek; Heui-Joo, Choi; Do-Hee, Ahn; Kwang-Rag, Kim; Minsoo, Lee; Sung-Paal, Yim; Hongsuk, Chung; Detroux, P.; Meessen, O.; Defloor, J.; Lars-Erik, Holm; Barescut, J.C.; Vacquier, B.; Laurier, D.; Caer, S.; Quesne, B.; Oudalova, A.; Geras' kin, St.; Dikarev, V.; Dikareva, N.; Chernonog, E.; Yang-Geun, Chung; Gab-Bock, Lee; Sun-Young, Bang; Yong-Sun, Lee; Bolognese-Milsztajn, T.; Frank, D.; Lacoste, V.; Pihet, P.; Lacronique, J.F.; Chauliac, C.; Verwaerde, D.; Pavageau, O.; Zaetta, A.; Varaine, F.; Warin, D.; Hudelot, J.P.; Bioux, Ph.; Klann, R.; Petruzzi, A.; D' auria, F.; Yung Kwon, Jin; Chul Jin, Chol; Mihalache, M.; Radu, V.; Pavelescu, M.; Schneidesch, Ch.R.; Jinzhao, Zhang; Dalleur, J.P.; Nuttin, A.; Meplan, O.; Wilson, J.; Perdu, F.; Campioni, G.; Mounier, C.; Sigrist, J.F.; Laine, Ch.; Broc, D.; Robbe, M.F.; Cariou, Y.; Seok-Kyun, Yoon; Win, Naing; Myung-Hyun, Kim; Kyung, Hee; Fridman, E.; Shwageraus, E.; Galperin, A.; Meplan, O.; Laulan, O.; Mechel-Sendis, F.; Belgaid, M.; Kadem, F.; Amokrane, A.; Hamidouche, T.; El-Khider, Si-Ahmed

    2005-11-15

    This issue gathers the abstracts of the papers presented at the ninth European nuclear conference (ENC-2005). The main part of the conference is split into 20 sessions. These sessions cover all technical aspects of nuclear power, from reactor design to waste management, without forgetting experimental and research reactors, reactor dismantling, economy, resources, safety, radioprotection and education issues. Perspectives of a nuclear renaissance are clearly visible in the world. This renaissance, mainly due to political, economical, societal and ecological factors, is fuelled by scientific and technical progress. This conference was the opportunity to present together these aspects of nuclear power and to analyze their mutual interactions.

  10. Sensitivity analysis

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003741.htm Sensitivity analysis To use the sharing features on this page, please enable JavaScript. Sensitivity analysis determines the effectiveness of antibiotics against microorganisms (germs) ...

  11. Main results of the Ouabain and Adducin for Specific Intervention on Sodium in Hypertension Trial (OASIS-HT: a randomized placebo-controlled phase-2 dose-finding study of rostafuroxin

    Directory of Open Access Journals (Sweden)

    Citterio Lorena

    2011-01-01

    Full Text Available Background The Ouabain and Adducin for Specific Intervention on Sodium in Hypertension (OASIS-HT Trial was a phase-2 dose-finding study of rostafuroxin, a digitoxygenin derivative, which selectively antagonizes the effects of endogenous ouabain (EO on Na+,K+-ATPase and mutated adducin. Rostafuroxin lowered blood pressure (BP in some animal models and in humans. Methods OASIS-HT consisted of 5 concurrently running double-blind cross-over studies. After 4 weeks without treatment, 435 patients with uncomplicated systolic hypertension (140-169 mm Hg were randomized to rostafuroxin (0.05, 0.15, 0.5, 1.5 or 5.0 mg/d or matching placebo, each treatment period lasting 5 weeks. The primary endpoint was the reduction in systolic office BP. Among the secondary endpoints were diastolic office BP, 24-h ambulatory BP, plasma EO concentration and renin activity, 24-h urinary sodium and aldosterone excretion, and safety. ANOVA considered treatment sequence (fixed effect, subjects nested within sequence (random, period (fixed, and treatment (fixed. Results Among 410 analyzable patients (40.5% women; mean age, 48.4 years, the differences in the primary endpoint (rostafuroxin minus placebo ranged from -0.18 mm Hg (P = 0.90 on 0.15 mg/d rostafuroxin to 2.72 mm Hg (P = 0.04 on 0.05 mg/d. In the 5 dosage arms combined, the treatment effects averaged 1.30 mm Hg (P = 0.03 for systolic office BP; 0.70 mm Hg (P = 0.08 for diastolic office BP; 0.36 mm Hg (P = 0.49 for 24-h systolic BP; and 0.05 mm Hg (P = 0.88 for 24-h diastolic BP. In the 2 treatment groups combined, systolic (-1.36 mm Hg and diastolic (-0.97 mm Hg office BPs decreased from week 5 to 10 (P for period effect ≤0.028, but carry-over effects were not significant (P ≥ 0.11. All other endpoints were not different on rostafuroxin and placebo. Minor side-effects occurred with similarly low frequency on rostafuroxin and placebo. Conclusions In 5 concurrently running double-blind cross-over studies

  12. PREFACE: Quark Matter 2006 Conference Quark Matter 2006 Conference

    Science.gov (United States)

    Ma, Yu-Gang; Wang, En-Ke; Cai, Xu; Huang, Huan-Zhong; Wang, Xin-Nian; Zhu, Zhi-Yuan

    2007-07-01

    The Quark Matter 2006 conference was held on 14-20 November 2006 at the Shanghai Science Hall of the Shanghai Association of Sciences and Technology in Shanghai, China. It was the 19th International Conference on Ultra-Relativistic Nucleus-Nucleus Collisions. The conference was organized jointly by SINAP (Shanghai Institute of Applied Physics, Chinese Academy of Sciences (CAS)) and CCNU (Central China Normal University, Wuhan). Over 600 scientists from 32 countries in five continents attended the conference. This is the first time that China has hosted such a premier conference in the field of relativistic heavy-ion collisions, an important event for the Chinese high energy nuclear physics community. About one half of the conference participants are junior scientists—a clear indication of the vigor and momentum for this field, in search of the fundamental nature of the nuclear matter at extreme conditions. Professor T D Lee, honorary chair of the conference and one of the founders of the quark matter research, delivered an opening address with his profound and philosophical remarks on the recent discovery of the nature of strongly-interacting quark-gluon-plasma (sQGP). Professor Hongjie Xu, director of SINAP, gave a welcome address to all participants on behalf of the two hosting institutions. Dr Peiwen Ji, deputy director of the Mathematics and Physics Division of the Natural Science Foundation of China (NSFC), also addressed the conference participants and congratulated them on the opening of the conference. Professor Mianheng Jiang, vice president of the Chinese Academy of Sciences (CAS), gave a concise introduction about the CAS as the premier research institution in China. He highlighted continued efforts at CAS to foster international collaborations between China and other nations. The Quark Matter 2006 conference is an example of such a successful collaboration between high energy nuclear physicists in China and other nations all over the world. The

  13. 33rd Actinide Separations Conference

    Energy Technology Data Exchange (ETDEWEB)

    McDonald, L M; Wilk, P A

    2009-05-04

    Welcome to the 33rd Actinide Separations Conference hosted this year by the Lawrence Livermore National Laboratory. This annual conference is centered on the idea of networking and communication with scientists from throughout the United States, Britain, France and Japan who have expertise in nuclear material processing. This conference forum provides an excellent opportunity for bringing together experts in the fields of chemistry, nuclear and chemical engineering, and actinide processing to present and discuss experiences, research results, testing and application of actinide separation processes. The exchange of information that will take place between you, and other subject matter experts from around the nation and across the international boundaries, is a critical tool to assist in solving both national and international problems associated with the processing of nuclear materials used for both defense and energy purposes, as well as for the safe disposition of excess nuclear material. Granlibakken is a dedicated conference facility and training campus that is set up to provide the venue that supports communication between scientists and engineers attending the 33rd Actinide Separations Conference. We believe that you will find that Granlibakken and the Lake Tahoe views provide an atmosphere that is stimulating for fruitful discussions between participants from both government and private industry. We thank the Lawrence Livermore National Laboratory and the United States Department of Energy for their support of this conference. We especially thank you, the participants and subject matter experts, for your involvement in the 33rd Actinide Separations Conference.

  14. PREFACE: The Irago Conference 2012

    Science.gov (United States)

    Sandhu, Adarsh; Okada, Hiroshi

    2013-04-01

    The Irago Conference 2012 - 360 degree outlook on critical scientific and technological challenges for a sustainable society Organized by the Electronics-Inspired Interdisciplinary Research Institute (EIIRIS) at Toyohashi University of Technology, the Irago Conference, held recently (15-16 November) in Aichi, Japan, aimed to enhance mutual understanding between scientists, engineers and policymakers. Over 180 participants tackled topics ranging from energy and natural resources to public health and disaster prevention. The 360-degree outlook of the conference impressed speakers and guests. ''This conference has been extremely informative,'' noted Robert Gellar from the University of Tokyo. ''A unique conference with experts from a range of backgrounds,'' agreed Uracha Ruktanonchai from the National Nanotechnology Center (NANOTEC) in Thailand. Similarly, G P Li, professor of electrical engineering and computer science at the University of California Irvine commented that he had been ''able to think the unthinkable'' as a range of topics came together. The conference was streamed live on Ustream to ensure that researchers from across the world could benefit from thought-provoking presentations examining global issues such as energy, disaster mitigation and nanotechnology. ''This was wonderful,'' said Oussama Khatib from Stanford University, ''A good recipe of speakers from such a range of backgrounds.'' Manuscripts submitted to the organizers were peer-reviewed, and the papers in this proceedings were accepted for Journal of Physics: Conference Series. In addition to the formal speaker programme, graduate-student sessions provided a platform for graduate students to describe their latest findings as oral presentations. A series of excursions to relevant locations, such as the Tahara megasolar region under construction and a local car-manufacturing factory, gave participants the opportunity to further consider practical applications of their research in industry

  15. AINSE conference on radiation biology and chemistry. Conference handbook

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-12-31

    The conference handbook contains 60 oral and poster presentations dealing with recent advances in radiation chemistry applied to biological studies, radiopharmaceuticals, radiosensitizers as well as to solid state chemical physics.

  16. SIAM Conference on Computational Science and Engineering

    Energy Technology Data Exchange (ETDEWEB)

    None

    2003-01-01

    The Second SIAM Conference on Computational Science and Engineering was held in San Diego from February 10-12, 2003. Total conference attendance was 553. This is a 23% increase in attendance over the first conference. The focus of this conference was to draw attention to the tremendous range of major computational efforts on large problems in science and engineering, to promote the interdisciplinary culture required to meet these large-scale challenges, and to encourage the training of the next generation of computational scientists. Computational Science & Engineering (CS&E) is now widely accepted, along with theory and experiment, as a crucial third mode of scientific investigation and engineering design. Aerospace, automotive, biological, chemical, semiconductor, and other industrial sectors now rely on simulation for technical decision support. For federal agencies also, CS&E has become an essential support for decisions on resources, transportation, and defense. CS&E is, by nature, interdisciplinary. It grows out of physical applications and it depends on computer architecture, but at its heart are powerful numerical algorithms and sophisticated computer science techniques. From an applied mathematics perspective, much of CS&E has involved analysis, but the future surely includes optimization and design, especially in the presence of uncertainty. Another mathematical frontier is the assimilation of very large data sets through such techniques as adaptive multi-resolution, automated feature search, and low-dimensional parameterization. The themes of the 2003 conference included, but were not limited to: Advanced Discretization Methods; Computational Biology and Bioinformatics; Computational Chemistry and Chemical Engineering; Computational Earth and Atmospheric Sciences; Computational Electromagnetics; Computational Fluid Dynamics; Computational Medicine and Bioengineering; Computational Physics and Astrophysics; Computational Solid Mechanics and Materials; CS

  17. 6th International Cryocoolers Conference

    CERN Document Server

    Knox, Margaret

    1991-01-01

    Cryocoolers 6 archives developments and performance measurements in the field of cryocoolers based on the contributions of leading international experts at the 6th International Cryocooler Conference that was held in Plymouth, Massachusetts, on October 25-26, 1990. This year's conference consisted of 54 papers and was sponsored by the David Taylor Naval Ship Research and Development Center of Annapolis, Maryland. The conference proceedings containing 49 submitted manuscripts was published by the David Taylor Naval Ship Research and Development Center in the report reproduced here.

  18. 7th International Cryocooler Conference

    CERN Document Server

    1993-01-01

    Cryocoolers 7 archives developments and performance measurements in the field of cryocoolers based on the contributions of leading international experts at the 7th International Cryocooler Conference which was held in Santa Fe, New Mexico, on November 17-19, 1992. This year's conference consisted of over 100 papers and was hosted by the Nichols Research Corp. and the Air Force Phillips Laboratory of Albuquerque, New Mexico. The conference proceedings were published by the Air Force Phillips Laboratory in the four-volume set reproduced here.

  19. International Conference on Cosmic Rays

    CERN Multimedia

    W.O. LOCK

    1964-01-01

    Towards the end of last year the 8th International conference on cosmic rays, held under the auspices of the International Union of Pure and Applied Physics (I.U.P.A.P.) and the Department of Atomic Energy of the Government of India, was held at Jaipur, India. Among the participants was W.O. Lock, head of CERN's Emulsion Group, who gave an invited talk on recent work in the field of what is normally known as high-energy physics — though in the context of this conference such energies seem quite low. In this article, Dr. Lock gives a general review of the conference and of the subjects discussed.

  20. Conference on High Energy Physics

    CERN Document Server

    2016-01-01

    Conference on High Energy Physics (HEP 2016) will be held from August 24 to 26, 2016 in Xi'an, China. This Conference will cover issues on High Energy Physics. It dedicates to creating a stage for exchanging the latest research results and sharing the advanced research methods. HEP 2016 will be an important platform for inspiring international and interdisciplinary exchange at the forefront of High Energy Physics. The Conference will bring together researchers, engineers, technicians and academicians from all over the world, and we cordially invite you to take this opportunity to join us for academic exchange and visit the ancient city of Xi’an.

  1. International Conference on Algebraic Topology

    CERN Document Server

    Cohen, Ralph; Miller, Haynes; Ravenel, Douglas

    1989-01-01

    These are proceedings of an International Conference on Algebraic Topology, held 28 July through 1 August, 1986, at Arcata, California. The conference served in part to mark the 25th anniversary of the journal Topology and 60th birthday of Edgar H. Brown. It preceded ICM 86 in Berkeley, and was conceived as a successor to the Aarhus conferences of 1978 and 1982. Some thirty papers are included in this volume, mostly at a research level. Subjects include cyclic homology, H-spaces, transformation groups, real and rational homotopy theory, acyclic manifolds, the homotopy theory of classifying spaces, instantons and loop spaces, and complex bordism.

  2. DOE Workshop at Tapia Conference

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, Valerie [Texas A & M Univ., College Station, TX (United States)

    2015-02-19

    The DE-SC0013568 DOE Grant, in the amount of $11,822.79, was used to support five doctoral students from underrepresented groups to attend the 2015 Richard Tapia Celebration of Diversity in Computing Conference, held February 18-21 in Boston, MA. Each scholarship was approximately $1200 to cover conference registration, travel, and lodging for the duration of the conference. The remaining $5,822.79 was used to support a DOE Breakfast Workshop during breakfast on Thursday, February 19. The Breakfast supported approximately 140 graduate students from underrepresented groups to learn about the different career opportunities at the different DOE National Laboratories.

  3. AINSE`s 40th anniversary conference. Conference handbook

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-31

    Highlights of 40 years of activity of the Australian Institute of Nuclear Science and Engineering (AINSE) were the main focus of this conference. Topics covered include nuclear physics, plasma physics, radiation chemistry, radiation biology, neutron diffraction, nuclear techniques of analysis and other relevant aspects of nuclear science and technology. The conference handbook contains the summaries of the 78 papers and posters presented and the list of participants

  4. PREFACE: Quark Matter 2006 Conference

    Science.gov (United States)

    Ma, Yu-Gang; Wang, En-Ke; Cai, Xu; Huang, Huan-Zhong; Wang, Xin-Nian; Zhu, Zhi-Yuan

    2007-07-01

    The Quark Matter 2006 conference was held on 14 20 November 2006 at the Shanghai Science Hall of the Shanghai Association of Sciences and Technology in Shanghai, China. It was the 19th International Conference on Ultra-Relativistic Nucleus Nucleus Collisions. The conference was organized jointly by SINAP (Shanghai Institute of Applied Physics, Chinese Academy of Sciences (CAS)) and CCNU (Central China Normal University, Wuhan). Over 600 scientists from 32 countries in five continents attended the conference. This is the first time that China has hosted such a premier conference in the field of relativistic heavy-ion collisions, an important event for the Chinese high energy nuclear physics community. About one half of the conference participants are junior scientists—a clear indication of the vigor and momentum for this field, in search of the fundamental nature of the nuclear matter at extreme conditions. Professor T D Lee, honorary chair of the conference and one of the founders of the quark matter research, delivered an opening address with his profound and philosophical remarks on the recent discovery of the nature of strongly-interacting quark-gluon-plasma (sQGP). Professor Hongjie Xu, director of SINAP, gave a welcome address to all participants on behalf of the two hosting institutions. Dr Peiwen Ji, deputy director of the Mathematics and Physics Division of the Natural Science Foundation of China (NSFC), also addressed the conference participants and congratulated them on the opening of the conference. Professor Mianheng Jiang, vice president of the Chinese Academy of Sciences (CAS), gave a concise introduction about the CAS as the premier research institution in China. He highlighted continued efforts at CAS to foster international collaborations between China and other nations. The Quark Matter 2006 conference is an example of such a successful collaboration between high energy nuclear physicists in China and other nations all over the world. The

  5. 18th International Cryocooler Conference

    CERN Document Server

    Ross, Ronald G

    2014-01-01

    Cryocoolers 18 Cryocoolers 18 archives developments and performance measurements in the field of cryocoolers based on the contributions of leading international experts at the 18th International Cryocooler Conference that was held in Syracuse, New York, on June 9-12, 2014. The program of this conference lead to the 76 peer-reviewed papers that are published here. Over the years the International Cryocoolers Conference has become the preeminent worldwide conference for the presentation of the latest developments and test experiences with cryocoolers. The typical applications of this technology include cooling space and terrestrial infrared focal plane arrays, space x-ray detectors, medical applications, and a growing number of high-temperature superconductor applications.

  6. 10. international mouse genome conference

    Energy Technology Data Exchange (ETDEWEB)

    Meisler, M.H.

    1996-12-31

    Ten years after hosting the First International Mammalian Genome Conference in Paris in 1986, Dr. Jean-Louis Guenet presided over the Tenth Conference at the Pasteur Institute, October 7--10, 1996. The 1986 conference was a satellite to the Human Gene Mapping Workshop and had approximately 50 attendees. The 1996 meeting was attended by 300 scientists from around the world. In the interim, the number of mapped loci in the mouse increased from 1,000 to over 20,000. This report contains a listing of the program and its participants, and two articles that review the meeting and the role of the laboratory mouse in the Human Genome project. More than 200 papers were presented at the conference covering the following topics: International mouse chromosome committee meetings; Mutant generation and identification; Physical and genetic maps; New technology and resources; Chromatin structure and gene regulation; Rate and hamster genetic maps; Informatics and databases; and Quantitative trait analysis.

  7. Waterfowl disease conference report : 1980

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A Waterfowl Disease Conference was held in Denver the fall of 1980 to develop a better program for dealing with waterfowl disease problems. Most of the report dealt...

  8. Climate Leadership Awards and Conference

    Science.gov (United States)

    The seventh annual Climate Leadership Awards Dinner will be held during the 2018 Climate Leadership Conference; the event publicly recognize individuals and organizations for their outstanding leadership in reducing greenhouse gas emissions.

  9. International Conference on Robot Ethics

    CERN Document Server

    Sequeira, Joao; Tokhi, Mohammad; Kadar, Endre; Virk, Gurvinder

    2017-01-01

    This book contains the Proceedings of the International Conference on Robot Ethics, held in Lisbon on October 23 and 24, 2015. The conference provided a multidisciplinary forum for discussing central and evolving issues concerning safety and ethics that have arisen in various contexts where robotic technologies are being applied. The papers are intended to promote the formulation of more precise safety standards and ethical frameworks for the rapidly changing field of robotic applications. The conference was held at Pavilhão do Conhecimento/Ciência Viva in Lisbon and brought together leading researchers and industry representatives, promoting a dialogue that combines different perspectives and experiences to arrive at viable solutions for ethical problems in the context of robotics. The conference topics included but were not limited to emerging ethical, safety, legal and societal problems in the following domains: • Service/Social Robots: Robots performing tasks in human environments and involving close ...

  10. European Metals Conference

    CERN Document Server

    Vereecken, Jean

    1991-01-01

    This volume contains the papers that will be presented at 'EMC '91 '-the European Metals Conference-to be held in Brussels, Belgium, from 15 to 20 September 1991, and organized by Benelux Metallurgie, GDMB (Gesellschaft Deutscher Metallhutten­ und Bergleute) and IMM (the Institution of Mining and Metallurgy). 'EMC '91' is the first of an intended major series organized at the European level with the aim of bringing together all those who are involved with the extraction and processing of non-ferrous metals-European metallurgists and their international colleagues-to provide them with the opportunity to exchange views on the state and evolution of their industry. The programme covers all the different aspects of the metallurgy of non-ferrous metals from mining to fabricated products. Particular attention is being paid to the European non -ferrous industry with respect to changes in demand, the technology used, pressures on the environment and the competitive position of manufacturers. The contributions of the...

  11. 4th Machining Innovations Conference

    CERN Document Server

    2014-01-01

    This contributed volume contains the research results presented at the 4th Machining Innovations Conference, Hannover, September 2013. The topic of the conference are new production technologies in aerospace industry and the focus is on energy efficient machine tools as well as sustainable process planning. The target audience primarily comprises researchers and experts in the field but the book may also be beneficial for graduate students.

  12. A National Airport Conference Held

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    <正>A national working conference on civil airport was held at Ramada Pudong Airport Hotel on August 10. At the two-day conference, CAAC Minister Yang Yuanyuan and Shanghai Vice-Mayor Yang Xiong delivered speeches. Yang Guoqing, vice minister of CAAC, gave a working report on upgrading China’s civil airports.Yang Yuanyuan required all the airports to adhere to the

  13. 9. international mouse genome conference

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-31

    This conference was held November 12--16, 1995 in Ann Arbor, Michigan. The purpose of this conference was to provide a multidisciplinary forum for exchange of state-of-the-art information on genetic mapping in mice. This report contains abstracts of presentations, focusing on the following areas: mutation identification; comparative mapping; informatics and complex traits; mutagenesis; gene identification and new technology; and genetic and physical mapping.

  14. Topics in Number Theory Conference

    CERN Document Server

    Andrews, George; Ono, Ken

    1999-01-01

    From July 31 through August 3,1997, the Pennsylvania State University hosted the Topics in Number Theory Conference. The conference was organized by Ken Ono and myself. By writing the preface, I am afforded the opportunity to express my gratitude to Ken for beng the inspiring and driving force behind the whole conference. Without his energy, enthusiasm and skill the entire event would never have occurred. We are extremely grateful to the sponsors of the conference: The National Sci­ ence Foundation, The Penn State Conference Center and the Penn State Depart­ ment of Mathematics. The object in this conference was to provide a variety of presentations giving a current picture of recent, significant work in number theory. There were eight plenary lectures: H. Darmon (McGill University), "Non-vanishing of L-functions and their derivatives modulo p. " A. Granville (University of Georgia), "Mean values of multiplicative functions. " C. Pomerance (University of Georgia), "Recent results in primality testing. " C. ...

  15. PREFACE: XXI Fluid Mechanics Conference

    Science.gov (United States)

    Szmyd, Janusz S.; Fornalik-Wajs, Elzbieta; Jaszczur, Marek

    2014-08-01

    This Conference Volume contains the papers presented at the 21st Fluid Mechanics Conference (XXI FMC) held at AGH - University of Science and Technology in Krakow, Poland, 15-18 June 2014, and accepted for Proceedings published in the Journal of Physics: Conference Series. The Fluid Mechanics Conferences have been taking place every two years since 1974, a total of forty years. The 21st Fluid Mechanics Conference (XXI FMC) is being organized under the auspices of the Polish Academy of Sciences, Committee of Mechanics. The goal of this conference is to provide a forum for the exposure and exchange of ideas, methods and results in fluid mechanics. Conference topics include, but are not limited to Aerodynamics, Atmospheric Science, Bio-Fluids, Combustion and Reacting Flows, Computational Fluid Dynamics, Experimental Fluid Mechanics, Flow Machinery, General Fluid Dynamics, Hydromechanics, Heat and Fluid Flow, Measurement Techniques, Micro- and Nano- Flow, Multi-Phase Flow, Non-Newtonian Fluids, Rotating and Stratified Flows, Turbulence. Within the general subjects of this conference, the Professor Janusz W. Elsner Competition for the best fluid mechanics paper presented during the Conference is organized. Authors holding a M.Sc. or a Ph.D. degree and who are not older than 35 years of age may enter the Competition. Authors with a Ph.D. degree must present individual papers; authors with a M.Sc. degree may present papers with their supervisor as coauthor, including original results of experimental, numerical or analytic research. Six state-of-the-art keynote papers were delivered by world leading experts. All contributed papers were peer reviewed. Recommendations were received from the International Scientific Committee, reviewers and the advisory board. Accordingly, of the 163 eligible extended abstracts submitted, after a review process by the International Scientific Committee, 137 papers were selected for presentation at the 21st Fluid Mechanics Conference, 68

  16. LIGHT AND ELECTRON-MICROSCOPIC DEMONSTRATION OF THE OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT P-NITROPHENYLPHOSPHATASE ACTIVITY (K-NPPASE) USING A CE-MG-DOUBLE CAPTURE TECHNIQUE

    NARCIS (Netherlands)

    GLIESING, M; KALICHARAN, D; HALBHUBER, KJ

    The cerium-based method of Kobayashi et al. for the histochemical demonstration of K-NPPase activity was improved. Besides Ce3+ additionally Mg2+ ions as orthophosphate capture were employed (double capture technique). For light microscopical purposes the Mg-phosphate was converted into Ce-phosphate

  17. Conference Proceedings: Photography and Britishness

    Directory of Open Access Journals (Sweden)

    Sean Willcock

    2016-11-01

    Full Text Available The video-recordings presented here were made at the conference Photography and Britishness, held at the Yale Center for British Art on November 4 – 5, 2016. The conference was the result of a collaboration between the Yale Center for British Art, New Haven, the Paul Mellon Centre for Studies in British Art, London, and the Huntington Library, Art Collections, and Botanical Gardens in San Marino—three research institutions that have a converging interest in British art. The conference sought to investigate the various ways in which notions of “Britishness” have been communicated, inflected, and contested through the photographic image. It was not a conference about the history of photography in Britain, or about British photography. Rather, it sought to consider the nature of the relationship between photography and Britishness: the notion that photography can capture images of Britishness, at the same time that our sense of what Britishness constitutes is produced by the photographic image. A key question for the conference was whether Britishness can have a photographic referent—or whether it is itself an effect of representation. Speakers at the conference approached these questions from a wide range of perspectives and focusing on a diverse number of photographic materials—from family albums and studio portraits to advertisements, reportage, and aerial photography—which demonstrated the complexities and instabilities not only of the term Britishness, but also of the medium of photography. The conference was opened with an introduction by John Tagg. The videos included here are presented in the order they were delivered.

  18. Annual International DIC Society Conference and SEM Fall Conference

    CERN Document Server

    Reu, Phillip

    2017-01-01

    This collection represents a single volume of technical papers presented at the Annual International DIC Society Conference and SEM Fall Conference organized by the Society for Experimental Mechanics and Sandia National Laboratories and held in Philadelphia, PA, November 7-10, 2016. The volume presents early findings from experimental, standards development and various other investigations concerning digital image correlation - an important area within Experimental Mechanics. The area of Digital Image Correlation has been an integral track within the SEM Annual Conference spearheaded by Professor Michael Sutton from the University of South Carolina. In 2016, the SEM and Sandia joined their collaborative strengths to launch a standing fall meeting focusing specifically on developments in the area of Digital Image Correlation. The contributed papers within this volume span numerous technical aspects of DIC including standards development for the industry. .

  19. IEEE Sensors 2004 Conference

    Science.gov (United States)

    2004-10-27

    feature from the transient response for the features. M2P-P4: The characterization of nanostructured copper- doped tin oxide films for gas sensor...microarrays - Just to note they used RF magnetron spluttered technique to produce SnO2- CuO sensors that were sensitive towards NH3 (this is a gas of...sensors based on nanoparticle W03 thick films They used commercial W03 nanopowder from Aldrich, this was mixed with InCl3 or BiCI3 and screen printed onto

  20. International Conference on Computational Mechanics

    CERN Document Server

    Atluri, Satya

    1986-01-01

    It is often said that these days there are too many conferences on general areas of computational mechanics. mechanics. and numer ical methods. vJhile this may be true. the his tory of scientific conferences is itself quite short. According to Abraham Pais (in "Subtle is the Lord ...• " Oxford University Press. 1982. p.80). the first international scientific conference ever held was the Karlsruhe Congress of Chemists. 3-5 September 1860 in Karlsruhe. Germany. There were 127 chemists in attendance. and the participants came from Austria. Belgium. France. Germany. Great Britain. Italy. Mexico. Poland. Russia. Spain. Sweden. and Switzerland. At the top of the agenda of the points to be discussed at this conference was the question: "Shall a difference be made between the expressions molecule and atom?" Pais goes on to note: "The conference did not at once succeed in bringing chemists closer together ... It is possible that the older men were offended by the impetuous behavior and imposing manner of the younger...

  1. QCD@LHC International Conference

    CERN Document Server

    2016-01-01

    The particle physics groups of UZH and ETH will host the QCD@LHC2016 conference (22.8.-26.8., UZH downtown campus), which is part of an annual conference series bringing together theorists and experimentalists working on hard scattering processes at the CERN LHC, ranging from precision studies of Standard Model processes to searches for new particles and phenomena. The format of the conference is a combination of plenary review talks and parallel sessions, with the latter providing a particularly good opportunity for junior researchers to present their results. The conference will take place shortly after the release of the new data taken by the LHC in sping 2016 at a collision energy of 13TeV, expected to more than double the currently available data set. It will be one of the first opportunities to discuss these data in a broader context, and we expect the conference to become a very lively forum at the interface of phenomenology and experiment.

  2. Climate Sensitivity

    Energy Technology Data Exchange (ETDEWEB)

    Lindzen, Richard [M.I.T.

    2011-11-09

    Warming observed thus far is entirely consistent with low climate sensitivity. However, the result is ambiguous because the sources of climate change are numerous and poorly specified. Model predictions of substantial warming aredependent on positive feedbacks associated with upper level water vapor and clouds, but models are notably inadequate in dealing with clouds and the impacts of clouds and water vapor are intimately intertwined. Various approaches to measuring sensitivity based on the physics of the feedbacks will be described. The results thus far point to negative feedbacks. Problems with these approaches as well as problems with the concept of climate sensitivity will be described.

  3. Protective effects of ouabain conjugated peptide from Ph. D.-7 Library on vascular endothelial cell%从噬菌体7肽库中筛选的哇巴因结合肽对血管内皮细胞的保护作用

    Institute of Scientific and Technical Information of China (English)

    徐忠伟; 徐瑞成; 陈小义; 刘英富

    2009-01-01

    AIM: To find one kind of peptide that will conjugate with ouabain and inhibit its biological function, and provide a new treatment strategy for primary hypertension. METHODS: In this study, ouabain was used as a target to screen ouabain conjugated peptide (OCP) from Ph. D. -7 phage display peptide library. After 3 rounds of bio-panning, the products were identified by ELISA and DNA electrophoresis analysis and sequencing. Peptide was synthesized and analyzed the activity by radioligand binding assay. The inhibitory ratio of cell proliferation was measured by MTT and the cell morphology changing was measured by Hoechst 33342/PI staining. The expression of Na~+-K~+-ATPase α1-subunit and β1-subunit were detected by RT-PCR and immunocytochemistry. The levels of the free intracellular Na~+ in EAhy926 cells were measured by laser confocal microscope. RESULTS: The ouabain conjugated peptide was found out, and it was occupied in 0.64(9/14). The analysis of protein showed that the obtained peptides had no homology with Na~+-K~+-ATPase. The amino acid sequence of synthesized peptide was Arg-Cys-Met-Thr-Ser-Arg-Ser. There was binding activity between OCP and ~3H-ouabain. The MTT assay showed that OCP could reverse the inhibition action of ouabain on vascular endothelial EAhy926 cells in a dose and time-dependent manner. The number of apoptotic cells had significantly decreased detected by Hoechst 33342/PI staining. The results of RT-PCR and immunocytochemistry showed that OCP could inhibit the up-regulated expression of Na~+-K~+-ATPase α1-subunit and down-regulated expression of Na~+-K~+-ATPase β1-subunit induced by ouabain in EAhy926 cells. CONCLUSION: The OCP could reverse the growth inhibition and death induction of ouabain in EAhy926 cells, which would provide the basis for studying the interaction between ouabain and Na~+-K~+-ATPase and explore novel anti-ouabain agents.%目的:寻找与哇巴因高亲和力结合并抑制其生物功能的相关小分子肽,为治

  4. Gluten Sensitivity

    Science.gov (United States)

    Gluten is a protein found in wheat, rye, and barley. It is found mainly in foods but ... products like medicines, vitamins, and supplements. People with gluten sensitivity have problems with gluten. It is different ...

  5. 37th International MATADOR Conference

    CERN Document Server

    Li, Lin

    2013-01-01

    Presented here are 97 refereed papers given at the 37th MATADOR Conference held at The University of Manchester in July 2012. The MATADOR series of conferences covers the topics of Manufacturing Automation and Systems Technology, Applications, Design, Organisation and Management, and Research.   The proceedings of this conference contain original papers contributed by researchers from many countries on different continents. The papers cover the principles, techniques and applications in aerospace, automotive, biomedical, energy, consumable goods and process industries.    The papers in this volume reflect: the importance of manufacturing to international wealth creation; the emerging fields of micro- and nano-manufacture; the increasing trend towards the fabrication of parts using lasers; the growing demand for precision engineering and part inspection techniques, and the changing trends in manufacturing within a global environment. .

  6. Positivity VII (Zaanen Centennial Conference)

    CERN Document Server

    Pagter, Ben; Gaans, Onno; Veraar, Mark

    2016-01-01

    This book presents the proceedings of Positivity VII, held from 22-26 July 2013, in Leiden, the Netherlands. Positivity is the mathematical field concerned with ordered structures and their applications in the broadest sense of the word. A biyearly series of conferences is devoted to presenting the latest developments in this lively and growing discipline. The lectures at the conference covered a broad spectrum of topics, ranging from order-theoretic approaches to stochastic processes, positive solutions of evolution equations and positive operators on vector lattices, to order structures in the context of algebras of operators on Hilbert spaces. The contributions in the book reflect this variety and appeal to university researchers in functional analysis, operator theory, measure and integration theory and operator algebras. Positivity VII was also the Zaanen Centennial Conference to mark the 100th birth year of Adriaan Cornelis Zaanen, who held the chair of Analysis in Leiden for more than 25 years and was ...

  7. International Conference GIREP EPEC 2015

    CERN Document Server

    Dębowska, Ewa

    2017-01-01

    This book presents a selection of the best contributions to GIREP EPEC 2015, the Conference of the International Research Group on Physics Teaching (GIREP) and the European Physical Society’s Physics Education Division (EPS PED). It introduces readers interested in the field to the problem of identifying strategies and tools to improve physics teaching and learning so as to convey Key Competences and help students acquire them. The main topic of the conference was Key Competences (KC) in physics teaching and learning in the form of knowledge, skills and attitudes that are fundamental for every member of society. Given the role of physics as a field strongly connected not only to digital competence but also to several other Key Competences, this conference provided a forum for in-depth discussions of related issues.

  8. International Conference on Health Informatics

    CERN Document Server

    2014-01-01

    This volume presents the proceedings of the International Conference on Health Informatics (ICHI). The conference was a new special topic conference initiative by the International Federation of Medical and Biological Engineering (IFMBE), held in Vilamoura, Portugal on 7-9 November, 2013. The main theme of the ICHI2013 was “Integrating Information and Communication Technologies with Biomedicine for Global Health”. The proceedings offer a unique forum to examine enabling technologies of sensors, devices and systems that optimize the acquisition, transmission, processing, storage, retrieval of biomedical and health information as well as to report novel clinical applications of health information systems and the deployment of m-Health, e-Health, u-Health, p-Health and Telemedicine.

  9. International Conference on Underwater Environment

    CERN Document Server

    Jaulin, Luc; Creuze, Vincent; Debese, Nathalie; Quidu, Isabelle; Clement, Benoît; Billon-Coat, Annick

    2016-01-01

    This volume constitutes the results of the International Conference on Underwater Environment, MOQESM’14, held at “Le Quartz” Conference Center in Brest, France, on October 14-15, 2014, within the framework of the 9th Sea Tech Week, International Marine Science and Technology Event. The objective of MOQESM'14 was to bring together researchers from both academia and industry, interested in marine robotics and hydrography with application to the coastal environment mapping and underwater infrastructures surveys. The common thread of the conference is the combination of technical control, perception, and localization, typically used in robotics, with the methods of mapping and bathymetry. The papers presented in this book focus on two main topics. Firstly, coastal and infrastructure mapping is addressed, focusing not only on hydrographic systems, but also on positioning systems, bathymetry, and remote sensing. The proposed methods rely on acoustic sensors such as side scan sonars, multibeam echo sounders, ...

  10. Groups and Geometries : Siena Conference

    CERN Document Server

    Kantor, William; Lunardon, Guglielmo; Pasini, Antonio; Tamburini, Maria

    1998-01-01

    On September 1-7, 1996 a conference on Groups and Geometries took place in lovely Siena, Italy. It brought together experts and interested mathematicians from numerous countries. The scientific program centered around invited exposi­ tory lectures; there also were shorter research announcements, including talks by younger researchers. The conference concerned a broad range of topics in group theory and geometry, with emphasis on recent results and open problems. Special attention was drawn to the interplay between group-theoretic methods and geometric and combinatorial ones. Expanded versions of many of the talks appear in these Proceedings. This volume is intended to provide a stimulating collection of themes for a broad range of algebraists and geometers. Among those themes, represented within the conference or these Proceedings, are aspects of the following: 1. the classification of finite simple groups, 2. the structure and properties of groups of Lie type over finite and algebraically closed fields of f...

  11. Conference on Environmental Data Management

    CERN Document Server

    Oppenheimer, Dorothy; Brogden, William; Environmental Data Management

    1976-01-01

    Throughout the world a staggering amount of resources have been used to obtain billions of environmental data points. Some, such as meteorological data, have been organized for weather map display where many thousands of data points are synthesized in one compressed map. Most environmental data, however, are still widely scattered and generally not used for a systems approach, but only for the purpose for which they were originally taken. These data are contained in relatively small computer programs, research files, government and industrial reports, etc. This Conference was called to bring together some of the world's leaders from research centers and government agencies, and others concerned with environmental data management. The purpose of the Conference was to organize discussion on the scope of world environmental data, its present form and documentation, and whether a systematic approach to a total system is feasible now or in the future. This same subject permeated indirectly the Stockholm Conference...

  12. Fifth International Conference KSE 2013

    CERN Document Server

    Denoeux, Thierry; Tran, Dang; Le, Anh; Pham, Son; Knowledge and Systems Engineering

    2014-01-01

    The field of Knowledge and Systems Engineering (KSE) has experienced rapid development and inspired many applications in the world of information technology during the last decade. The KSE conference aims at providing an open international forum for presentation, discussion and exchange of the latest advances and challenges in research of the field. These proceedings contain papers presented at the Fifth International Conference on Knowledge and Systems Engineering (KSE 2013), which was held in Hanoi, Vietnam, during 17–19 October, 2013. Besides the main track of contributed papers, which are compiled into the first volume, the conference also featured several special sessions focusing on specific topics of interest as well as included one workshop, of which the papers form the second volume of these proceedings. The book gathers a total of 68 papers describing recent advances and development on various topics including knowledge discovery and data mining, natural language processing, expert systems, intell...

  13. International Conference on Risk Analysis

    CERN Document Server

    Oliveira, Teresa; Rigas, Alexandros; Gulati, Sneh

    2015-01-01

    This book covers the latest results in the field of risk analysis. Presented topics include probabilistic models in cancer research, models and methods in longevity, epidemiology of cancer risk, engineering reliability and economical risk problems. The contributions of this volume originate from the 5th International Conference on Risk Analysis (ICRA 5). The conference brought together researchers and practitioners working in the field of risk analysis in order to present new theoretical and computational methods with applications in biology, environmental sciences, public health, economics and finance.

  14. Chapman Conference on Rainfall Fields

    Science.gov (United States)

    Gupta, V. K.

    The Chapman Conference on Rainfall Fields, sponsored by AGU, was the first of its kind; it was devoted to strengthening scientific interaction between the North American and Latin American geophysics communities. It was hosted by Universidad Simon Bolivar and Instituto Internacional de Estudios Avanzados, in Caracas, Venezuela, during March 24-27, 1986. A total of 36 scientists from Latin America, the United States, Canada, and Europe participated. The conference, which was convened by I. Rodriguez-Iturbe (Universidad Simon Bolivar) and V. K. Gupta (University of Mississippi, University), brought together hydrologists, meteorologists, and mathematicians/statisticians in the name of enhancing an interdisciplinary focus on rainfall research.

  15. 2nd Tourism Postdisciplinarity Conference

    DEFF Research Database (Denmark)

    Following the noted success of the 1st international conference on postdisciplinary approaches to tourism studies (held in Neuchatel, Switzerland, 19-22 June, 2013), we are happy to welcome you to the 2nd Tourism Postdisciplinarity Conference. Postdisciplinarity surpasses the boundaries...... of study less embedded in that system of thought. Postdisciplinarity is an epistemological endeavour that speaks of knowledge production and the ways in which the world of physical and social phenomena can be known. It is also an ontological discourse as it concerns what we call ‘tourism...

  16. Customised DSS and decision conferences

    DEFF Research Database (Denmark)

    Barfod, Michael Bruhn; Leleur, Steen

    2009-01-01

    This paper presents and exemplifies a combination of techniques for deriving and modelling decision-maker and/or stakeholder preferences using a decision conference process. The applied techniques are used for the development of customised decision support systems (C-DSS) which can be used...... for appraisals of large transport infrastructure projects. The paper exemplifies how the process at a decision conference can be effectively supported by a DSS customised using appropriate techniques for the specific task in hand. In this respect a conventional cost-benefit analysis (CBA) is combined...

  17. PHYSICS FOR HEALTH: CONFERENCE HIGHLIGHTS

    CERN Multimedia

    2016-01-01

    Highlights of ICTR-PHE 2016 - International Conference on Translational Research in Radio-Oncology and Physics for Health -, co organized by CERN, aims at developing new strategies to better diagnose and treat cancer, by uniting biology and physics with clinics. Through the various sessions and symposia, the scientific programme offers the delegates the opportunity to discuss, in a friendly atmosphere, the latest progress in physics breakthroughs for health applications. The third edition of this conference took place at CICG (Centre International de Conférence Genève) from 15 to 19 Feb 2016.

  18. First Stars III Conference Summary

    CERN Document Server

    O'Shea, Brian W; Heger, Alexander; Abel, Tom

    2008-01-01

    The understanding of the formation, life, and death of Population III stars, as well as the impact that these objects had on later generations of structure formation, is one of the foremost issues in modern cosmological research and has been an active area of research during the past several years. We summarize the results presented at "First Stars III," a conference sponsored by Los Alamos National Laboratory, the Kavli Institute for Particle Astrophysics and Cosmology, and the Joint Institute for Nuclear Astrophysics. This conference, the third in a series, took place in July 2007 at the La Fonda Hotel in Santa Fe, New Mexico, U.S.A.

  19. Nineteenth annual actinide separations conference: Conference program and abstracts

    Energy Technology Data Exchange (ETDEWEB)

    Bronson, M. [ed.

    1995-12-31

    This report contains the abstracts from the conference presentations. Sessions were divided into the following topics: Waste treatment; Spent fuel treatment; Issues and responses to Defense Nuclear Facility Safety Board 94-1; Pyrochemical technologies; Disposition technologies; and Aqueous separation technologies.

  20. Living green : Conference proceedings of the living green scientific conference

    NARCIS (Netherlands)

    Kersten, W.C.

    2013-01-01

    Presentaties van Livinggreen Scientific Conference: Stimulating energy efficiency in households - Comparison of the Livinggreen.eu methods to theory – Daphne Geelen Engaging households in sustainable renovation – Exploration of a complementary approach - Meijer, S.A., Geelen, D.V., Franken, V., Kers

  1. Conferences in Context: Status, Communication, and Evaluation

    Science.gov (United States)

    Wagner, L. Wesley; Brinkerhoff, Merlin

    1975-01-01

    The relationship between managers and three variables are examined: time commitments of managers to staff conferences, status or rank of managers, and the quality of communicative exchange in conference settings. (Author/DEP)

  2. International conference on defects in insulating crystals

    Energy Technology Data Exchange (ETDEWEB)

    1977-01-01

    Short summaries of conference papers are presented. Some of the conference topics included transport properties, defect levels, superionic conductors, radiation effects, John-Teller effect, electron-lattice interactions, and relaxed excited states. (SDF)

  3. 1995 International Sherwood Fusion Theory Conference

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-07-01

    This book is a guide to the 1995 International Sherwood Fusion Theory Conference. It consists largely of abstracts of the oral and poster presentations that were to be made, and gives some general information about the conference and its schedule.

  4. CERN to host conference on information society

    CERN Multimedia

    CERN will host a conference on the Role of Science in the Information Society (RSIS) in December. This conference will focus on ensuring that the information society benefits people to the greatest extent possible, especially in developing regions.

  5. Autism's 'Worryingly' High Suicide Rates Spur Conference

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_165946.html Autism's 'Worryingly' High Suicide Rates Spur Conference Signs of ... News) -- High rates of suicide among people with autism are drawing specialists to a conference this week ...

  6. Path Sensitization

    Institute of Scientific and Technical Information of China (English)

    赵著行; 闵应骅; 等

    1997-01-01

    For different delay models,the concept of sensitization can be very different.Traditonal concepts of sensitization cannot precisely describe circuit behavior when the input vectors change very fast.Using Boolean process aporoach,this paper presents a new definition of sensitization for arbitrary input waveforms.By this new concept it is found that if the inputs of a combinational circuit can change at any time,and each gate's delay varies within an interval (bounded gate delay model),then every path,which is not necessarily a single topological path,is sensitizable.From the experimental results it can be seen that,all nonsensitizable paths for traditional concepts actually can propagate transitions along them for some input waveforms.However,specified time between input transitions(STBIT) and minimum permissible pulse width(ε)are two major factors to make some paths non-sensitizable.

  7. Training or vacation? The academic conference tourism

    OpenAIRE

    Veloutsou, Cleopatra; Chreppas, Chrisovalantis

    2015-01-01

    The current study concentrates on factors affecting the intentions of academics attending an academic conference. It highlights the importance of academic conferences and academic conference tourism and discusses meetings, the convention industry and also their utility in the career development of academics. Through qualitative research and a review of the literature on conference tourism push and pull motivation factors are suggested. The power of these factors to predict the intention to at...

  8. Conference on Islamic Accounting and Finance

    OpenAIRE

    Hasan AYAYDIN

    2016-01-01

    Abstract. Hasan Kalyoncu Univeristy organized first of its conference series as “Conference on Islamic Accounting and Finance” themed on October 27-28, 2016 in Gaziantep, Turkey. Conference on Islamic Accounting and Finance aims to establish an academic forum for scholars, researchers and practitioners to exchange their ideas about aspects of Islamic accounting, auditing, business ethics and financial reporting. Submitted papers as well as presentations and discussions at the conference were ...

  9. Conferences as a Dramaturgical Learning Space

    DEFF Research Database (Denmark)

    Hansen, Nicoline Jacoby

    Arguing that conferences are an important but under-researched and under-developed dimension of continuing education, the paper proposes a notion of conferences as a dramatic learning space. Using the design-based research methodology, a theoretical framework drawing on adult learning theories...... and dramaturgy is developed, consisting of four design principles: rhythm, reflection, involvement and interaction. These are sought implemented in a specific conference program, the case of the ECCI X conference, and the final program is explained and discussed....

  10. Gordon Research Conference on Electrodeposition

    Science.gov (United States)

    2006-08-04

    ORME LAWRENCE LIVERMORE NATIONAL Speaker Registered LABORATORY ANDRE A PASA DEPARTAMENTO DE FISICA - UFSC Poster Presenter Registered GYANARANJAN...deposited at the electrode surface from molecular and ionic precursors in solution. The morphology and orientation of electrodeposited films can be precisely...topics at this year’s conference are molecular electronics, nucleation and growth, epitaxial electrodeposition, shape control, magnetic materials

  11. Business Meets Biodiversity Conference 2012

    NARCIS (Netherlands)

    Vollaard, B.; Man, M. de; Verweij, P.A.

    2012-01-01

    How can companies successfully integrate the sustainable management of ecosystems and biodiversity into their business models? This was the central question at the international conference ‘Business Meets Biodiversity’ held in Utrecht, The Netherlands, on June 27th 2012. The organizing committee, co

  12. Business Meets Biodiversity Conference 2012

    NARCIS (Netherlands)

    Vollaard, B.; Man, M. de; Verweij, P.A.

    2012-01-01

    How can companies successfully integrate the sustainable management of ecosystems and biodiversity into their business models? This was the central question at the international conference ‘Business Meets Biodiversity’ held in Utrecht, The Netherlands, on June 27th 2012. The organizing committee,

  13. Solar 78 Northwest conference proceedings

    Energy Technology Data Exchange (ETDEWEB)

    None

    1978-01-01

    Thirty eight papers are included. One was abstracted previously. Separate entries were prepared for thirty-seven. Also included are the conference evaluation summary, Pacific Northwest Solar Energy Association organization information, lists of commercial and non-commercial exhibitors, speakers and stearing committee members, and attendees. (MHR)

  14. 3rd International Algebra Conference

    CERN Document Server

    Fong, Yuen; Zelmanov, Efim

    2003-01-01

    This volume contains one invited lecture which was presented by the 1994 Fields Medal­ ist Professor E. Zelmanov and twelve other papers which were presented at the Third International Conference on Algebra and Their Related Topics at Chang Jung Christian University, Tainan, Republic of China, during the period June 26-July 1, 200l. All papers in this volume have been refereed by an international referee board and we would like to express our deepest thanks to all the referees who were so helpful and punctual in submitting their reports. Thanks are also due to the Promotion and Research Center of National Science Council of Republic of China and the Chang Jung Christian University for their generous financial support of this conference. The spirit of this conference is a continuation of the last two International Tainan­ Moscow Algebra Workshop on Algebras and Their Related Topics which were held in the mid-90's of the last century. The purpose of this very conference was to give a clear picture of the rece...

  15. Conference of CAS History Commission

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ The Commission of CAS History held its third conference on Oct. 31 in Beijing. CAS Vice-President Yang Bailing and Vice-Secretary of the CPC Leading Group at CAS Guo Chuanjie addressed the meeting, which was chaired by Guo Huadong, CAS vice secretary -general.

  16. Gordon Conference on Microbial Adhesion

    Science.gov (United States)

    1988-07-01

    Sweden Al fa-Laval, Sweden Eli Lilly Monsanto Barnes-Hind Labs Bristol -Myers Pfizer Gillette Following the conference, many positive comments were...clays and metals: R. Mitchell, discussion leader -’S- F. Dazzo, Attachment of Rhizobium trifolii to 9:00-9:45 a.m. white clover root hairs. E. Bayer

  17. What Good Are Conferences, Anyway?

    Science.gov (United States)

    Pietro, David C.

    1996-01-01

    According to Frederick Herzberg's studies of employee motivation, humans are driven by motivating factors that allow them to grow psychologically and hygiene factors that help them meet physical needs. Good education conferences can enhance both factors by helping principals refocus their energies, exchange ideas with trusted colleagues, and view…

  18. IEEE Conference Publications in Libraries.

    Science.gov (United States)

    Johnson, Karl E.

    1984-01-01

    Conclusions of surveys (63 libraries, OCLC database, University of Rhode Island users) assessing handling of Institute of Electrical and Electronics Engineers (IEEE) conference publications indicate that most libraries fully catalog these publications using LC cataloging, and library patrons frequently require series access to publications. Eight…

  19. IEEE Conference Publications in Libraries.

    Science.gov (United States)

    Johnson, Karl E.

    1984-01-01

    Conclusions of surveys (63 libraries, OCLC database, University of Rhode Island users) assessing handling of Institute of Electrical and Electronics Engineers (IEEE) conference publications indicate that most libraries fully catalog these publications using LC cataloging, and library patrons frequently require series access to publications. Eight…

  20. IUPAC conference on chemical thermodynamics

    Energy Technology Data Exchange (ETDEWEB)

    1984-01-01

    The 39th Calorimetry Conference includes papers on the following typics: thermodynamic properties of various compounds and materials; measuring methods and equipment for determination of thermodynamic quantities; calculation of thermodynamic quantities. Fossil fuels, nuclear fuels, electrochemistry, photochemistry, and biochemistry are areas of applications which are emphasized.

  1. CIEE 1993 annual conference: Program

    Energy Technology Data Exchange (ETDEWEB)

    1993-08-01

    The California Institute for Energy efficiency`s third annual conference highlights the results of CIEE-sponsored multiyear research in three programs: Building Energy Efficiency, Air Quality Impacts of Energy Efficiency, and End-Use Resource Planning. Results from scoping studies, Director`s discretionary research, and exploratory research are also featured.

  2. National Hospitality Conference 2016: a Report

    OpenAIRE

    Murphy, James Peter

    2015-01-01

    The 2015 National Hospitality Conference took place recently at the Intercontinental Hotel, Ballsbridge, Dublin. The main theme of the 2015 conference was ‘Challenges and Opportunities’ , DIT Bar Studies students from the School of Culinary Arts & Food Technology joined delegates in debating and discussing the significant issues surrounding the conference theme. This report highlights the issues discussed.

  3. National Hospitality Conference 2014: a Report

    OpenAIRE

    Murphy, James Peter

    2014-01-01

    The 2014 National Hospitality Conference took place on November 3rd and was held at the Four Seasons Hotel, Ballsbridge, Dublin. The main theme of this year’s conference was ‘Preparing for growth’ , DIT Bar Studies students from the School of Culinary Arts & Food Technology joined delegates in debating and discussing the significant issues surrounding the conference theme.

  4. Conference-Courses for Academic Credit.

    Science.gov (United States)

    Bobes, Marvin

    1979-01-01

    The Wayne State University Studies/Weekend College Program demonstrates a cost effective, flexible model for conference courses. Designed to give adult, part-time students short-term intensive learning experiences, the conference course adapts typical conference activities (guest speakers, films, workshops) to a single or multidisciplinary theme,…

  5. 7 CFR 1.167 - Conference.

    Science.gov (United States)

    2010-01-01

    ... Proceedings Under Section 2 of the Capper-Volstead Act § 1.167 Conference. (a) Purpose. Upon motion of a party... that conducting the conference by audio-visual telecommunication: (i) Is necessary to prevent prejudice... in the conference: (i) Is necessary to prevent prejudice to a party; (ii) Is necessary because of...

  6. Hawking radiation conference, book of proceedings

    CERN Document Server

    Mersini-Houghton, Laura

    2016-01-01

    Proceedings of the 'Hawking Radiation' conference in Stockholm, Sweden 2015. It includes a link to the video recording of the conference and all the talks, discussions, and communications, that took place during the week of the conference . We hope the recorded discussions will be helpful, especially to the current and future young researchers and students.

  7. West Tennessee ACEI 2006 Fall Conference

    Science.gov (United States)

    Clifford, Anna; Hailey, Beth

    2007-01-01

    This article presents the West Tennessee ACEI 2006 Fall Conference held at Union University in Jackson, Tennessee on October 14, 2006. The conference theme, Turning the Pages: A Focus on Children's Literature, was emphasized throughout the day. During the conference, the early childhood classroom teachers, preservice teachers, and administrators…

  8. Do market participants learn from conference calls?

    NARCIS (Netherlands)

    Roelofsen, E.; Verbeeten, F.; Mertens, G.

    2014-01-01

    We examine whether market participants learn from the information that is disseminated during the Q-and-A section of conference calls. Specifically, we investigate whether stock prices react to information on intangible assets provided during conference calls, and whether conference calls interactio

  9. Fourth European Conference on Mechanism Science (EUCOMES 2012 Conference)

    CERN Document Server

    Ceccarelli, Marco; New Trends in Mechanism and Machine Science : Theory and Applications in Engineering

    2013-01-01

    This book contains the papers of the European Conference on Mechanisms Science (EUCOMES 2012 Conference). The book presents the most recent research developments in the mechanism and machine science field and their applications. Topics addressed are theoretical kinematics, computational kinematics, mechanism design, experimental mechanics, mechanics of robots, dynamics of machinery, dynamics of multi-body systems, control issues of mechanical systems, mechanisms for biomechanics, novel designs, mechanical transmissions, linkages and manipulators, micro-mechanisms, teaching methods, history of mechanism science and industrial and non-industrial applications. This volume will also serve as an interesting reference for European activity in the fields of Mechanism and Machine Science as well as a source of inspiration for future works and developments.

  10. Population conference: consensus and conflict.

    Science.gov (United States)

    Willson, P D

    1984-01-01

    The United Nations-sponsored International Conference on Population held in Mexico City was both a rejection and an affirmation of a new policy of the Reagan administration. The policy denies international family planning funds to nongovernmental organizations that perform or actively promote abortion as a family planning method in other nations. A compromise statement was accepted urging governments to take appropriate measures to discourage abortion as a family planning method and when possible to provide for the humane treatment and counseling of women ho resorted to abortion. The statement on abortion was 1 of 88 reccomendations approved by the conference. The commitment expressed in the 10-year-old World Population Plan of Action to the rights and responsiblity to all people as reaffirmed. The conference also endorsed family life education and sex education as well as suitable family planning, information and services for adolescents, with due consideration given to the role, rights and obligations of parents. Increased support for international population and family planning programs was urged and World Bank President, Clausen, urged a 4-fold increase in international funding by the year 2000. Most of the conference's recommendations re devoted to the broad range of population policy issues, including morbidity and mortality, international and internal migration, the relationship between population and economic development and the status of women. The purpose of the recommendations is to increase the momentum of international support. The Mexico City conference was characterized by a remarkable degree of consensus about population policies with respect to integration with economic development, the need to respect individual rights and the recognition that all nations have sovereign rights to develop and implement their own population policies. Conflict and controversy arose in the areas of the arms race and the Middle East. The US position on abortion funding

  11. CERN automatic audio-conference service

    CERN Multimedia

    Sierra Moral, R

    2009-01-01

    Scientists from all over the world need to collaborate with CERN on a daily basis. They must be able to communicate effectively on their joint projects at any time; as a result telephone conferences have become indispensable and widely used. Managed by 6 operators, CERN already has more than 20000 hours and 5700 audio-conferences per year. However, the traditional telephone based audio-conference system needed to be modernized in three ways. Firstly, to provide the participants with more autonomy in the organization of their conferences; secondly, to eliminate the constraints of manual intervention by operators; and thirdly, to integrate the audio-conferences into a collaborative working framework. The large number, and hence cost, of the conferences prohibited externalization and so the CERN telecommunications team drew up a specification to implement a new system. It was decided to use a new commercial collaborative audio-conference solution based on the SIP protocol. The system was tested as the first Euro...

  12. Sensitive innovation

    DEFF Research Database (Denmark)

    Søndergaard, Katia Dupret

    Present paper discusses sources of innovation as heterogenic and at times intangible processes. Arguing for heterogeneity and intangibility as sources of innovation originates from a theoretical reading in STS and ANT studies (e.g. Callon 1986, Latour 1996, Mol 2002, Pols 2005) and from field work...... in the area of mental health (Dupret Søndergaard 2009, 2010). The concept of sensitive innovation is developed to capture and conceptualise exactly those heterogenic and intangible processes. Sensitive innovation is therefore primarily a way to understand innovative sources that can be......, but are not necessarily, recognized and acknowledged as such in the outer organisational culture or by management. The added value that qualifies these processes to be defined as “innovative” are thus argued for along different lines than in more traditional innovation studies (e.g. studies that build on the classic...

  13. PREFACE: International Conference "Trends in Spintronics and Nanomagnetism" (TSN-2010)

    Science.gov (United States)

    Maruccio, Giuseppe; Sanvito, Stefano; Hoffmann, Germar; Wiesendanger, Roland; Rowan, Alan

    2011-03-01

    Conference banner The International Conference "Trends in Spintronics and Nanomagnetism" (TSN-2010), was organized by partners of the EU-project SpiDME and held in the historical city of Lecce, Italy from 23-27 May 2010, at the Ecotekne Campus, University of Salento. The conference provided an international forum to discuss recent progress and future trends in the field. In particular, the aim was to bring together the community of more conventional spin-transport, with that of molecular and nano-magnetism. The main topics of TSN-2010 were: MATERIALSSPIN-PHYSICS AND THEORY - Molecular Magnets- Spin injection - Magnetic nanoparticles and nanowires- Domain walls, spin torque and vortex dynamics - Magnetic semiconductors- Numerical modeling of organic nanomagnetism - Multiferroics and transition metal oxides APPLICATIONSADVANCES IN CHARACTERIZATION - Magnetic Multilayers- Magneto-optical characterization and spin manipulation - Spin-photonics- Intrinsic spin transport mechanism in organics - Molecular and nano-spintronics- Organometallic molecules on surfaces - Spin-based quantum computation- Single molecular magnets on surfaces - Magnetism for sensing and nanomedicine- Nanoscale characterization and spin-sensitive SPM The scientific programme started on Monday 24 May and ended on Thursday 27 May. The Nobel Laureate A Fert attended the conference giving a plenary talk and the programme also featured invited presentations by (in alphabetical order): M Aeschlimann, M Affronte, N Atodiresei, P A Bobbert, A Dediu, N Kioussis, L W Molenkamp, J Moodera, V Prigodin, M Ruben, R Sessoli, R Tan, and H Wende. TSN2010 had 150 attendees who came from around the globe to present their latest research in 100 oral presentations. Contributed talks were selected by the program committee, composed of Giuseppe Maruccio, Ross Rinaldi, Valentina Arima, Fabio Della Sala, Maurizio Martino (Universitá del Salento, NNL Institute Nanoscience-CNR, Lecce, Italy), Stefano Sanvito (Trinity College

  14. Eighth International Conference on Paleoceanography

    Science.gov (United States)

    Grousset, Francis; Peterson, Larry; Delaney, Peggie; Elderfield, Harry; Emeis, Kay; Haug, Gerald; Stocker, Thomas; Wang, Pinxian

    Every three years since 1983, the paleoceanographic community has come together at a different venue to share new data and discoveries at the International Conference on Paleoceanography (ICP). For the recent ICP-8, France was the host country for a conference focused on the theme of “An Ocean View of Global Change.” The Environnements et Paleoenvironnement Oceanique (EPOC) paleoceanography group of the University Bordeaux I acted as the local organizing committee.Scientific presentations at ICP-8 addressed the latest discoveries in paleoceanography and highlighted both emerging and as-yet-unsolved questions on global climate change. Thirty-five speakers, invited by the ICP-8 Science Committee, gave overview talks during morning sessions organized around five major scientific themes. These themes were Cenozoic-Mesozoic Oceans; Carbonate and Silica Systems of the Pleistocene Ocean; Biogeochemical Cycles of the Past; High-Frequency Climate Variability; and Interhemispheric Ocean-Continent-Climate Linkages.

  15. 37th National Systems Conference

    CERN Document Server

    Yadav, Sandeep; Adhikari, Bibhas; Seshadri, Harinipriya; Fulwani, Deepak

    2015-01-01

    The book is a collection of peer-reviewed scientific papers submitted by active researchers in the 37th National System Conference (NSC 2013). NSC is an annual event of the Systems Society of India (SSI), primarily oriented to strengthen the systems movement and its applications for the welfare of humanity. A galaxy of academicians, professionals, scientists, statesman and researchers from different parts of the country and abroad are invited to attend the conference. The book presents research articles in the areas of system’s modelling, complex network modelling, cyber security, sustainable systems design, health care systems, socio-economic systems, and clean and green technologies. The book can be used as a tool for further research.

  16. Beyond AI: Artificial Dreams Conference

    CERN Document Server

    Zackova, Eva; Kelemen, Jozef; Beyond Artificial Intelligence : The Disappearing Human-Machine Divide

    2015-01-01

    This book is an edited collection of chapters based on the papers presented at the conference “Beyond AI: Artificial Dreams” held in Pilsen in November 2012. The aim of the conference was to question deep-rooted ideas of artificial intelligence and cast critical reflection on methods standing at its foundations.  Artificial Dreams epitomize our controversial quest for non-biological intelligence, and therefore the contributors of this book tried to fully exploit such a controversy in their respective chapters, which resulted in an interdisciplinary dialogue between experts from engineering, natural sciences and humanities.   While pursuing the Artificial Dreams, it has become clear that it is still more and more difficult to draw a clear divide between human and machine. And therefore this book tries to portrait such an image of what lies beyond artificial intelligence: we can see the disappearing human-machine divide, a very important phenomenon of nowadays technological society, the phenomenon which i...

  17. Solvay Conference on Surface Science

    CERN Document Server

    1988-01-01

    The articles collected in this volume give a broad overview of the current state of surface science. Pioneers in the field and researchers met together at this Solvay Conference to discuss important new developments in surface science, with an emphasis on the common area between solid state physics and physical chemistry. The contributions deal with the following subjects: structure of surfaces, surface science and catalysis, two-dimensional physics and phase transitions, scanning tunneling microscopy, surface scattering and surface dynamics, chemical reactions at surfaces, solid-solid interfaces and superlattices, and surface studies with synchrotron radiation. On each of these subjects an introductory review talk and a number of short research contributions are followed by extensive discussions, which appear in full in the text. This nineteenth Solvay Conference commemorates the 75th anniversary of the Solvay Institutes.

  18. 12th Reinventing Space Conference

    CERN Document Server

    2017-01-01

    The 2014 Reinventing Space conference presented a number of questions in the context of a constantly innovating space industry, from addressing the future of global cooperation, investigating the impact of cuts in US government spending on the private space sector, and probing the overall future of the commercial launch sector. Space tourism and new technology promise the revival of interest in space development (the Apollo Era was the first period of intense space activity and growth). The need to create dramatically lower cost, responsive and reliable launch systems and spacecraft has never been more vital. Advances in technology are allowing smaller and cheaper satellites to be orbited - from cubesats to nanosatellites to femtosatellites. Thanks to more efficient new launch possibilities, low cost access to space is becoming ever more achievable. Commercial companies and countries are targeting the industry with new funding. Organised by the British Interplanetary Society, the presentations at this confere...

  19. International Conference Approximation Theory XV

    CERN Document Server

    Schumaker, Larry

    2017-01-01

    These proceedings are based on papers presented at the international conference Approximation Theory XV, which was held May 22–25, 2016 in San Antonio, Texas. The conference was the fifteenth in a series of meetings in Approximation Theory held at various locations in the United States, and was attended by 146 participants. The book contains longer survey papers by some of the invited speakers covering topics such as compressive sensing, isogeometric analysis, and scaling limits of polynomials and entire functions of exponential type. The book also includes papers on a variety of current topics in Approximation Theory drawn from areas such as advances in kernel approximation with applications, approximation theory and algebraic geometry, multivariate splines for applications, practical function approximation, approximation of PDEs, wavelets and framelets with applications, approximation theory in signal processing, compressive sensing, rational interpolation, spline approximation in isogeometric analysis, a...

  20. Comparison between kinetics of positive inotropism of ibopamine and ouabain%异波帕胺与哇巴因的正性肌力作用的动力学比较

    Institute of Scientific and Technical Information of China (English)

    黄珺; 戴茵; 仇怡堂; 洪旭; 黄嘉梓; 于锋; 戴德哉

    1996-01-01

    目的:比较异波帕胺(Ibo)与哇巴因(Oua)正性肌力作用药效的动力学.方法:以离体猫右心室乳头状肌递增药浓观察正肌效应.最大反应是药物递增到发生自发收缩前的反应.以1g[E/Emax-E]对lg C得Hill方程.结果:Hill方程中S值:Ibo及Oua分别为1.16与1.97,Ibo的S值小于Oua,使Cg5/C5(产生95%及5%最大药效的药浓)Ibo(264)是Oua(22.9)的11倍.与Oua相比:Ibo起效浓度低,斜率小及最大药效的浓度高.药浓下降时,Ibo的药效-药浓曲线呈现逆时针滞后襻,比Oua更明显.结论:在药浓增加及下降时,Ibo的正肌效应的变化比Oua为小.%AIM: To compare the kinetics of positive inotropism between ibopamine (Ibo) and ouabain (Qua). METHODS: The isolated right papillary muscle of cat was used to assess the positive inotropic effects by cummulative concentrations of Ibo and Oua. The maximal effects was recognized as the contraction just before the appearance of spontaneous contraction. A Hill equation was developed by using lg [E/(Emax-E)] vs lg C. RESULTS - The 5 value of Ibo was smaller than that of Oua, causing C95/C5 (ratio of concentrations producing 95 %/5 % ofEmax) of Ibo (264) elevenfold as large as Oua (22. 9). Comparing with Oua, concentrations of Ibo initiating effects were lower, the slope was smaller, and higher concentrations were required for producing maximal effect. During drug concentration declining, the effect-concentration curve of Ibo showed a counter-hysteresis loop, more remarkable than Oua. CONCLUSION: The changes in positive inotropism of Ibo against drug concentrations were less than those of Oua.

  1. The H{sub 1}–H{sub 2} domain of the α{sub 1} isoform of Na{sup +}–K{sup +}–ATPase is involved in ouabain toxicity in rat ventricular myocytes

    Energy Technology Data Exchange (ETDEWEB)

    Xiong, Chen; Li, Jun-xia; Guo, Hui-cai; Zhang, Li-nan; Guo, Wei; Meng, Jing; Wang, Yong-li, E-mail: wangyongli@gmail.com

    2012-07-01

    The composition of different isoforms of Na{sup +}-K{sup +}-ATPase (NKA, Na/K pump) in ventricular myocytes is an important factor in determining the therapeutic effect and toxicity of cardiac glycosides (CGs) on heart failure. The mechanism whereby CGs cause these effects is still not completely clear. In the present study, we prepared two site-specific antibodies (SSA78 and WJS) against the H{sub 1}–H{sub 2} domain of α{sub 1} and α{sub 2} isoforms of NKA in rat heart, respectively, and compared their influences on the effect of ouabain (OUA) in isolated rat ventricular myocytes. SSA78 or WJS, which can specifically bind with the α{sub 1} or α{sub 2} isoform, were assessed with enzyme linked immunosorbent assay (ELISA), Western blot and immunofluorescent staining methods. Preincubation of myocytes with SSA78 inhibited low OUA affinity pump current but not high OUA affinity pump current, reduced the rise in cytosolic calcium concentration ([Ca{sup 2+}]{sub i}), attenuated mitochondrial Ca{sup 2+} overload, restored mitochondrial membrane potential reduction, and delayed the decrease of the myocardial contractile force as well as the occurrence of arrhythmic contraction induced by high concentrations (1 mM) but not low concentrations (1 μM) of OUA. Similarly, preincubation of myocytes with WJS inhibited high OUA affinity pump current, reduced the increase of [Ca{sup 2+}]{sub i} and the contractility induced by 1 μM but not that induced by 1 mM OUA. These results indicate that the H{sub 1}–H{sub 2} domain of the NKA α{sub 1} isoform mediates OUA-induced cardiac toxicity in rat ventricular myocytes, and inhibitors for this binding site may be used as an adjunct to CGs treatment for cardiovascular disease. -- Highlights: ► We prepared two antibodies against the H{sub 1}-H{sub 2} domain of α{sub 1} and α{sub 2} isoforms of NKA. ► The H{sub 1}-H{sub 2} domain of the NKA α{sub 1} isoform mediates OUA-induced cardiac toxicity. ► The H{sub 1}-H{sub 2

  2. 2015 Chinese Intelligent Automation Conference

    CERN Document Server

    Li, Hongbo

    2015-01-01

    Proceedings of the 2015 Chinese Intelligent Automation Conference presents selected research papers from the CIAC’15, held in Fuzhou, China. The topics include adaptive control, fuzzy control, neural network based control, knowledge based control, hybrid intelligent control, learning control, evolutionary mechanism based control, multi-sensor integration, failure diagnosis, reconfigurable control, etc. Engineers and researchers from academia, industry and the government can gain valuable insights into interdisciplinary solutions in the field of intelligent automation.

  3. 2015 Chinese Intelligent Systems Conference

    CERN Document Server

    Du, Junping; Li, Hongbo; Zhang, Weicun; CISC’15

    2016-01-01

    This book presents selected research papers from the 2015 Chinese Intelligent Systems Conference (CISC’15), held in Yangzhou, China. The topics covered include multi-agent systems, evolutionary computation, artificial intelligence, complex systems, computation intelligence and soft computing, intelligent control, advanced control technology, robotics and applications, intelligent information processing, iterative learning control, and machine learning. Engineers and researchers from academia, industry and the government can gain valuable insights into solutions combining ideas from multiple disciplines in the field of intelligent systems.

  4. Antibody Engineering and Therapeutics Conference

    OpenAIRE

    Larrick, James W; Parren, Paul WHI; Huston, James S; Plückthun, Andreas; Bradbury, Andrew; Tomlinson, Ian M; Chester, Kerry A.; Burton, Dennis R.; Adams, Gregory P.; Weiner, Louis M.; Scott, Jamie K.; Alfenito, Mark R; Veldman, Trudi; Reichert, Janice M.

    2013-01-01

    The Antibody Engineering and Therapeutics conference, which serves as the annual meeting of The Antibody Society, will be held in Huntington Beach, CA from Sunday December 8 through Thursday December 12, 2013. The scientific program will cover the full spectrum of challenges in antibody research and development, and provide updates on recent progress in areas from basic science through approval of antibody therapeutics. Keynote presentations will be given by Leroy Hood (Institute of System Bi...

  5. International Conference on Kaon Physics

    CERN Document Server

    2016-01-01

    The Conference follows former editions with similar emphasis on kaon physics. aiming at a comprehensive discussion on the latest experimental and theoretical achievements and development of new projects. TOPICS: CP and T violation CKM matrix and Flavor Mixing Rare decays Precise SM tests CPT and Quantum Mechanics Lepton universality and flavor violation Lattice gauge theory Chiral perturbation theory Physics beyond the Standard Model Future opportunities in Kaon Physics

  6. 2015 Chinese Intelligent Automation Conference

    CERN Document Server

    Li, Hongbo

    2015-01-01

    Proceedings of the 2015 Chinese Intelligent Automation Conference presents selected research papers from the CIAC’15, held in Fuzhou, China. The topics include adaptive control, fuzzy control, neural network based control, knowledge based control, hybrid intelligent control, learning control, evolutionary mechanism based control, multi-sensor integration, failure diagnosis, reconfigurable control, etc. Engineers and researchers from academia, industry and the government can gain valuable insights into interdisciplinary solutions in the field of intelligent automation.

  7. Fifth international fungus spore conference

    Energy Technology Data Exchange (ETDEWEB)

    Timberlake, W.E.

    1993-04-01

    This folio contains the proceedings of the Fifth International Fungal Spore Conference held August 17-21, 1991 at the Unicoi State Park at Helen, Georgia. The volume contains abstracts of each oral presentation as well as a collection of abstracts describing the poster sessions. Presentations were organized around the themes (1) Induction of Sporulation, (2) Nuclear Division, (3) Spore Formation, (4) Spore Release and Dispersal, and (4) Spore Germination.

  8. 5th European Turbulence Conference

    CERN Document Server

    1995-01-01

    Under the auspices of the Euromech Committee, the Fifth European Turbulence Conference was held in Siena on 5-8 July 1994. Following the previous ETC meeting in Lyon (1986), Berlin (1988), Stockholm (1990) and Delft (1992), the Fifth ETC was aimed at providing a review of the fundamental aspects of turbulence from a theoretical, numerical and experimental point of view. In the magnificent town of Siena, more than 250 scientists from all over the world, spent four days discussing new ideas on turbulence. As a research worker in the field of turbulence, I must say that the works presented at the Conference, on which this book is based, covered almost all areas in this field. I also think that this book provides a major opportunity to have a complete overview of the most recent research works. I am extremely grateful to Prof. C. Cercignani, Dr. M. Loffredo, and Prof. R. Piva who, as members of the local organizing committee, share the success of the Conference. I also want to thank Mrs. Liu' Catena, for her inva...

  9. 23rd CIRP design conference

    CERN Document Server

    Stark, Rainer

    2013-01-01

     The collection of papers in this book comprises the proceedings of the 23rd CIRP Design Conference held between March 11th and March 13th 2013 at the Ruhr-Universität Bochum in Germany. The event was organized in cooperation with the German Academic Society for Product Development – WiGeP. The focus of the conference was  on »Smart Product Engineering«, covering two major aspects of modern  product creation: the development of intelligent (“smart”) products as well as the new (“smart”) approach of engineering, explicitly taking into account consistent systems integration. Throughout the 98 papers contained in these proceedings, a range of topics are covered, amongst them the different facets and aspects of what makes a product or an engineering solution “smart”. In addition, the conference papers investigate new ways of engineering for production planning and collaboration towards Smart Product Engineering. The publications provide a solid insight into the pressing issues of modern digita...

  10. Real Time Conference 2016 Overview

    Science.gov (United States)

    Luchetta, Adriano

    2017-06-01

    This is a special issue of the IEEE Transactions on Nuclear Science containing papers from the invited, oral, and poster presentation of the 20th Real Time Conference (RT2016). The conference was held June 6-10, 2016, at Centro Congressi Padova “A. Luciani,” Padova, Italy, and was organized by Consorzio RFX (CNR, ENEA, INFN, Università di Padova, Acciaierie Venete SpA) and the Istituto Nazionale di Fisica Nucleare. The Real Time Conference is multidisciplinary and focuses on the latest developments in real-time techniques in high-energy physics, nuclear physics, astrophysics and astroparticle physics, nuclear fusion, medical physics, space instrumentation, nuclear power instrumentation, general radiation instrumentation, and real-time security and safety. Taking place every second year, it is sponsored by the Computer Application in Nuclear and Plasma Sciences technical committee of the IEEE Nuclear and Plasma Sciences Society. RT2016 attracted more than 240 registrants, with a large proportion of young researchers and engineers. It had an attendance of 67 students from many countries.

  11. 5th European Rheology Conference

    CERN Document Server

    1998-01-01

    Global sustainable development of the world economy requires better understanding and utilization of natural recourses. In this endeavor rheology has an indispensable role. The Rheology Conferences are therefore always an important event for science and technology. The Fifth European Rheology Conference, held from September 6 to 11 in the Portoro-z, Slovenia, will be the first AlI-European rheology meeting after the formal constitution of the European Society ofRheology. As such it will be a special historical event. At this meeting the European Society of Rheology will introduce the Weissenberg Medal, to be bestowed every four years to an individual for hislhers contribution to the field of Rheology. The recipient ofthe first award will be professor G. Marrucci ofthe Universita degli Studi di Napoli, Italy. Two mini Symposia will be part of the Conference. The first, on Industrial Rheology, will commemorate the late professor G. Astarita. The second will honor the eightieth birthday of professor N.W. Tschoeg...

  12. International conference centre, Geneva, Switzerland

    CERN Multimedia

    PH Department

    2009-01-01

    On 16-17 March 2009 the Energy Pact Foundation will be holding the Energy Pact Conference in Geneva. The Conference is organised with the support of the Republic and Canton of Geneva and has the Financial Times as its media partner. It will address for the first time in a comprehensive and integrated manner the key issues of energy needs and environmental and developmental challenges. Some 800 stakeholders and experts on these issues are expected. These will include high-level government officials, opinion leaders and representatives from the United Nations, NGOs, industry, civil society and the academic world. Gerhard Schröder, former Chancellor of Germany, will chair the Conference. Speakers with different backgrounds and expertise will include Dr. Carlo Rubia, Nobel Prize Winner, Ali Al-Naimi, Minister of Petroleum and Mineral Resources of Saudi Arabia, Gholam Hossein Nozari, Oil Minister of Iran, Gary Ross, CEO of PIRA Energy, a world-renowned energy market specialist, Ashok Khosla, President of the In...

  13. FOREWORD: HELAS II International Conference

    Science.gov (United States)

    Gizon, Laurent; Roth, Markus

    2008-07-01

    Volume 118 (2008) of Journal of Physics: Conference Series provides a written record of the talks and posters presented at the HELAS II International Conference `Helioseismology, Asteroseismology and MHD Connections'. The conference was held during the week 20-24 August 2007 in Göttingen, Germany, jointly hosted by the Max Planck Institute for Solar System Research and the Faculty of Physics of the University of Göttingen. A total of 140 scientists from all over the world attended. The Scientific Organizing Committee consisted of Conny Aerts, Annie Baglin, Jørgen Christensen-Dalsgaard, Thierry Corbard, Jadwiga Daszyńska-Daszkiewicz, Stefan Dreizler, Yvonne Elsworth, Laurent Gizon (Chairman), Wolfgang Glatzel, Frank Hill, Donald Kurtz, Oskar von der Lühe, Maria Pia Di Mauro, Mário Monteiro, Pere Pallé, Markus Roth, Philip Scherrer, Manfred Schüssler, and Michael Thompson. HELAS stands for the European Helio- and Asteroseismology Network, a Coordination Action supported by the sixth Framework Programme of the European Union. It aims to bring together researchers in the fields of solar and stellar oscillations. This volume consists of 91 articles organized into sections that reflect the scientific programme of the conference: 012001-07 Wave diagnostics in physics, geophysics and astrophysics 012008-09 Perspectives on helio- and asteroseismology 012010-17 Asteroseismology: Observations 012018-25 Asteroseismology: Theory 012026-32 Global helioseismology and solar models 012033-38 Local helioseismology and magnetic activity 012039-44 Future observational projects in helio- and asteroseismology 012045-91 Poster papers. The overwhelming majority of papers discuss the seismology of the Sun and stars. Papers in the first section provide a broader perspective on wave phenomena and techniques for probing other physical systems, from living beings to the universe as a whole. We were extremely fortunate to have particularly distinguished experts to cover these topics

  14. Creating Learning at Conferences Through Participant Involvement

    DEFF Research Database (Denmark)

    Ravn, Ib; Elsborg, Steen

    in Denmark to introduce a variety of simple learning techniques related to the design principles at thirty real conferences of some 100-200 participants each. We present twelve of these techniques and the data evaluating them and conclude that by spending a fraction of the time at a conference on involving......The typical conference is brimming with PowerPoint presentations that leave very little time for participant involvement. Students of learning have long abandoned the transfer model that underlies this massive show of one-way communication. We propose an alternative theory of the conference...... as a forum for learning, mutual inspiration and "human co-flourishing." We offer five design principles that specify how conferences may involve participants more and hence increase their learning. In the research and development effort reported here, our team collaborated with conference organizers...

  15. International Conference on Intelligence and Learning

    CERN Document Server

    Das, J; O’Connor, Neil

    1981-01-01

    This volume contains the Proceedings of an International Conference on Intelligence and Learning held at York University, England, on July 16-20, 1979. The conference was made possible with the support and assistance of the following agencies: NAT 0 Scientific Division, specifically the Human Factors panel, was the major sponsor of the conference. Special thanks are due to Dr. B. A. Bayraktar, who helped organize the conference. Special appreciation is also expressed for the support of the University of York where the conference was held, the University of Alberta, the University of California, Los Angeles, the Medical Research Council, especially its Developmental Psychology Research U nit in London, and the British Council. The conference was jointly directed by J. P. Das and N. 0' Connor. The directors appreciate the assistance in administrative matters of Patricia Chobater and Emma Collins of the University of Alberta. The Editors of the Proceedings acknowledge and appreciate the following individuals who...

  16. 4th International Conference on Advanced Robotics

    CERN Document Server

    1989-01-01

    The Fourth International Conference on Advanced Robotics was held in Columbus, Ohio, U. S. A. on June 13th to 15th, 1989. The first two conferences in this series were held in Tokyo. The third was held in Versailles, France in October 1987. The International Conference on Advanced Robotics is affiliated with the International Federation of Robotics. This conference was sponsored by The Ohio State University. The American Society of Mechanical Engineers was a cooperating co-sponsor. The objective of the International Conference on Advanced Robotics is to provide an international exchange of information on the topic of advanced robotics. This was adopted as one of the themes for international research cooperation at a meeting of representatives of seven industrialized countries held in Williamsburg, U. S. A. in May 1983. The present conference is truly international in character with contributions from authors of twelve countries. (Bulgaria, Canada, France, Great Britain, India, Italy, Japan, Peoples Republic o...

  17. Sensitivity, Recalculated

    Energy Technology Data Exchange (ETDEWEB)

    Brodsky, J. P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-11-07

    Prior to this study, the sensitivity in nEXO was calculated using an approximate method that does not provide 90% coverage. I find it useful to understand the confidence level of a limit by focusing on the confidence level as a property of the method used to find the limit.A particular method (algorithm,formula) is applied to an experiment to produce a limit. That experiment was conducted in a particular universe with certain values of the physical parameters. We’d like a method than produces a correct limit for 90% of the experiments that could run. We also want that to be true for every universe—that is, regardless of the physical parameter value, the method should still produce a correct limit 90% of the time.

  18. Beyond sensitivity

    DEFF Research Database (Denmark)

    Stott, Iain; Hodgson, David James; Townley, Stuart

    2012-01-01

    1. Perturbation analyses of population models are integral to population management: such analyses evaluate how changes in vital rates of members of the population translate to changes in population dynamics. Sensitivity and elasticity analyses of long-term (asymptotic) growth are popular...... formulae for the transfer function of population inertia, which describes nonlinear perturbation curves of transient population dynamics. The method comfortably fits into wider frameworks for analytical study of transient dynamics, and for perturbation analyses that use the transfer function approach. 3....... We use case studies to illustrate how the transfer function of population inertia may be used in population management. These show that strategies based solely on asymptotic perturbation analyses can cause undesirable transient dynamics and/ or fail to exploit desirable transient dynamics...

  19. 2 nd Mid-European Clay Conference

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The 2nd Mid-European Clay Conference (MECC'04) was held between 20-24th September 2004, in Miskolc, Hungary. The idea to hold common conferences was accepted by the national clay groups of four neighbouring countries, Poland, Slovakia, Hungary and Croatia, during the EUROCLAY Meeting in Cracow, Poland, in 1999. The first conference was held in 2001 at Stará Lesná, in the High Tatra Mts. in Slovakia.

  20. Annual Conference for National Trade Promotion

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ The 2008 National Trade Promotion Conference was held in Beijing on January 28th.Vice Premier Wu Yi attended the meeting and delivered an important speech.More than 300 delegates attended the conference,representing 88 local branches and industry branches of trade promotion system,as well as 16 overseas offices.CCPIT Chairman Wan Jifei has given an annual report at the conference.

  1. 5th International Conference on Cryocoolers

    CERN Document Server

    1989-01-01

    The Cryocoolers 5 proceedings archives the contributions of leading international experts at the 5th International Cryocooler Conference that was held in Monterey, California on August 18-19, 1988. The authors submitted twenty six papers describing advancements and applications of cryocoolers in the temperature range below 80K. This year's conference was hosted by the U.S. Naval Postgraduate School in Monterey, California, and the conference proceedings reproduced here were published by the Wright-Patterson AFB in Ohio.

  2. Report on the Taipei CHEP 2010 Conference

    CERN Document Server

    CERN. Geneva

    2010-01-01

    This year, the 18th Computing in High Energy and Nuclear Physics (CHEP) conference has been held at Taipei, Taiwan, from 18-22 October 2010.CHEP conferences provide an international forum to exchange information on computing experience and needs for the High Energy Physics and Nuclear Physics communities, and to review recent, ongoing and future activities.The speakers at this Computing Seminar will present their views of the CHEP 2010 conference.

  3. Defining the structural requirements for a helix in 23 S ribosomal RNA that confers erythromycin resistance

    DEFF Research Database (Denmark)

    Douthwaite, S; Powers, T; Lee, J Y

    1989-01-01

    deletion mutants show a sensitive phenotype. Deletions that extend into the base-pairing between GCC1208 and GGU1240 result in non-functional 23 S RNAs, which consequently do not confer resistance. A number of phylogenetically conserved nucleotides have been shown to be non-essential for 23 S RNA function...

  4. 2012 Gordon Research Conference, Electron donor-acceptor interactions, August 5-10 2012

    Energy Technology Data Exchange (ETDEWEB)

    McCusker, James [Michigan State Univ., East Lansing, MI (United States)

    2012-08-10

    The upcoming incarnation of the Gordon Research Conference on Electron Donor Acceptor Interactions will feature sessions on classic topics including proton-coupled electron transfer, dye-sensitized solar cells, and biological electron transfer, as well as emerging areas such as quantum coherence effects in donor-acceptor interactions, spintronics, and the application of donor-acceptor interactions in chemical synthesis.

  5. PREFACE AND CONFERENCE INFORMATION: Eighth International Conference on Laser Ablation

    Science.gov (United States)

    Hess, Wayne P.; Herman, Peter R.; Bäuerle, Dieter; Koinuma, Hideomi

    2007-04-01

    Laser ablation encompasses a wide range of delicate to extreme light interactions with matter that present considerably challenging problems for scientists to study and understand. At the same time, laser ablation also represents a basic process of significant commercial importance in laser material processing—defining a multi-billion dollar industry today. These topics were widely addressed at the 8th International Conference on Laser Ablation (COLA), held in Banff, Canada on 11-16 September 2005. The meeting took place amongst the majestic and natural beauty of the Canadian Rocky Mountains at The Banff Centre, where delegates enjoyed many inspiring presentations and discussions in a unique campus learning environment. The conference brought together world leading scientists, students and industry representatives to examine the basic science of laser ablation and improve our understanding of the many physical, chemical and/or biological processes driven by the laser. The multi-disciplinary research presented at the meeting underlies some of our most important trends at the forefront of science and technology today that are represented in the papers collected in this volume. Here you will find new processes that are producing novel types of nanostructures and nano-materials with unusual and promising properties. Laser processes are described for delicately manipulating living cells or modifying their internal structure with unprecedented degrees of control and precision. Learn about short-pulse lasers that are driving extreme physical processes on record-fast time scales and opening new directions from material processing applications. The conference papers further highlight forefront application areas in pulsed laser deposition, nanoscience, analytical methods, materials, and microprocessing applications. Laser ablation continues to grow and evolve, touching forefront areas in science and driving new technological trends in laser processing applications. Please

  6. Conference Attendees’ Satisfaction: Evidence from Belgrade (Serbia

    Directory of Open Access Journals (Sweden)

    Dunjić Jelena

    2012-10-01

    Full Text Available Conference industry brings significant economic effects and that is one of the reasons why many destinations around the world strive to organize conferences, especially the international ones, which make bigger economic effects. According to the Strategy of tourism development of the Republic of Serbia (2005-2015, city break and business tourism are tourism products of high priority, which can provide short-term positioning of Novi Sad and Belgrade, at the first place, at the international tourism market, and contribute to the growth of tourism turnover of foreign travellers.Belgrade is the capital and the largest city in Serbia. It is very well equipped with necessary infrastructure for organizing business events such as conferences, congresses, meetings etc. Lately, the number of international business events in Serbia is increasing and the majority of those events are organized in Belgrade. However, there are very few surveys which are examining satisfaction of the conference attendees in Serbia. This topic is often ignored despite the fact that the attendees satisfaction is substantial for organizers and all other relevant stakeholders at host destination. Therefore, the aim of this paper is to analyze the satisfaction of the conference attendees, as they are final consumers of conference tourist product and their experience regarding both conference and host destination is thus essential to destination marketing and management organizations, conference centres, hotel managers, meeting planners and all other stakeholders involved in conference industry and tourism

  7. Electronic Conference Tracking and Approval System

    Data.gov (United States)

    US Agency for International Development — eCTAS tracks information relevant to attendance and hosting conferences of USAID's Washington offices and Missions worldwide including cost, numbers attending,...

  8. Conference for Undergraduate Women in Physics

    Energy Technology Data Exchange (ETDEWEB)

    Bonnie Fleming

    2009-04-01

    The Yale Conference for Undergraduate Women in Physics was held on January 18th and 19th, 2008. The conference, targeted toward undergraduates in the Northeast, was a huge success. It was well attended by both a slate of impressive speakers including Janet Conrad, Mildred Dresselhaus, Elsa Garmire, Howard Georgi, Liz Rhodes, Meg Urry and Wendy Zhang, and many interested attendees. Talks were on current research, about issues for women in physics, and on the application process for graduate school. There was also a career panel, student talks, and a student poster session. The conference ran concurrently with the third annual conference at USC, as well as a first annual conference at the University of Michigan. Our purpose in creating this conference was to provide a supportive atmosphere for young physicists to connect with peers and with successful women in the field. We hope that from this conference, attendees have become confident and knowledgeable about applying to graduate school and be further inspired to pursue a career in physics. The following describes the conference program, participation and impact, logistics of running the conference and plans for the future.

  9. Applications of Computer Algebra Conference

    CERN Document Server

    Martínez-Moro, Edgar

    2017-01-01

    The Applications of Computer Algebra (ACA) conference covers a wide range of topics from Coding Theory to Differential Algebra to Quantam Computing, focusing on the interactions of these and other areas with the discipline of Computer Algebra. This volume provides the latest developments in the field as well as its applications in various domains, including communications, modelling, and theoretical physics. The book will appeal to researchers and professors of computer algebra, applied mathematics, and computer science, as well as to engineers and computer scientists engaged in research and development.

  10. Proceedings of NHA Annual Conferences

    Energy Technology Data Exchange (ETDEWEB)

    Debbi L. Smith

    2004-06-30

    The Proceedings of "Hydrogen: A Clean Energy Choice" and the 16th Annual U.S. Hydrogen Conference, "Partnering for the Global Hydrogen Future" include the presentations of high-level keynote speakers from the U.S. Department of Energy, the state government of California, Ambassadors and Executives of large corporations and emerging companies all presenting their vision on a future fueled by hydrogen. Parallel technical sessions informed attendees of developments in hydrogen technology R&D, commercial product development and market readiness. Persentations of the Student Design Competition Finalists are also included.

  11. International Conference on Robust Statistics

    CERN Document Server

    Filzmoser, Peter; Gather, Ursula; Rousseeuw, Peter

    2003-01-01

    Aspects of Robust Statistics are important in many areas. Based on the International Conference on Robust Statistics 2001 (ICORS 2001) in Vorau, Austria, this volume discusses future directions of the discipline, bringing together leading scientists, experienced researchers and practitioners, as well as younger researchers. The papers cover a multitude of different aspects of Robust Statistics. For instance, the fundamental problem of data summary (weights of evidence) is considered and its robustness properties are studied. Further theoretical subjects include e.g.: robust methods for skewness, time series, longitudinal data, multivariate methods, and tests. Some papers deal with computational aspects and algorithms. Finally, the aspects of application and programming tools complete the volume.

  12. International Conference on SEMS 2012

    CERN Document Server

    Liu, Chuang; Scientific explanation and methodology of science; SEMS 2012

    2014-01-01

    This volume contains the contributed papers of invitees to SEMS 2012 who have also given talks at the conference. The invitees are experts in philosophy of science and technology from Asia (besides China), Australia, Europe, Latin America, North America, as well as from within China. The papers in this volume represent the latest work of each researcher in his or her expertise; and as a result, they give a good representation of the cutting-edge researches in diverse areas in different parts of the world.

  13. Science and Information Conference 2014

    CERN Document Server

    Kapoor, Supriya; Bhatia, Rahul

    2015-01-01

    The book Intelligent Systems in Science and Information 2014 is  the carefully edited collection of 25 extended chapters from selected papers in the field of Computational Intelligence that , which received highly recommended feedback during the Science and Information Conference (SAI) 2014 review process. All chapters have gone through substantial extension and consolidation and were subject to another round of rigorous review and additional modification and represent the state of the art of the cutting-edge research and technologies in the related areas.

  14. 2013 Chinese Intelligent Automation Conference

    CERN Document Server

    Deng, Zhidong

    2013-01-01

    Proceedings of the 2013 Chinese Intelligent Automation Conference presents selected research papers from the CIAC’13, held in Yangzhou, China. The topics include e.g. adaptive control, fuzzy control, neural network based control, knowledge based control, hybrid intelligent control, learning control, evolutionary mechanism based control, multi-sensor integration, failure diagnosis, and reconfigurable control. Engineers and researchers from academia, industry, and government can gain an inside view of new solutions combining ideas from multiple disciplines in the field of intelligent automation. Zengqi Sun and Zhidong Deng are professors at the Department of Computer Science, Tsinghua University, China.

  15. 2013 Chinese Intelligent Automation Conference

    CERN Document Server

    Deng, Zhidong

    2013-01-01

    Proceedings of the 2013 Chinese Intelligent Automation Conference presents selected research papers from the CIAC’13, held in Yangzhou, China. The topics include e.g. adaptive control, fuzzy control, neural network based control, knowledge based control, hybrid intelligent control, learning control, evolutionary mechanism based control, multi-sensor integration, failure diagnosis, and reconfigurable control. Engineers and researchers from academia, industry, and government can gain an inside view of new solutions combining ideas from multiple disciplines in the field of intelligent automation.   Zengqi Sun and Zhidong Deng are professors at the Department of Computer Science, Tsinghua University, China.

  16. The AAPS National Biotechnology Conference.

    Science.gov (United States)

    Kenrick, Margaret

    2011-09-01

    Leading off the event, specialized workshops with panels of experts were conducted that brough together students and scientists to the current advances in their specialty. Delivery of therapeutics across the blood-brain barrier for the treatment of neurological conditions such as pain, Alzheimer's and Parkinson's disease is a field of great development with an expanding aging population of patients. Manoj Rajadhyaksha, Pfizer, Inc. and Saileta Prabhu, Genentech, Inc., brought together a panel describing the anatomy of the CNS and improvements in drug delivery. The conference that followed expanded upon the current approaches.

  17. International Conference Approximation Theory XIV

    CERN Document Server

    Schumaker, Larry

    2014-01-01

    This volume developed from papers presented at the international conference Approximation Theory XIV,  held April 7–10, 2013 in San Antonio, Texas. The proceedings contains surveys by invited speakers, covering topics such as splines on non-tensor-product meshes, Wachspress and mean value coordinates, curvelets and shearlets, barycentric interpolation, and polynomial approximation on spheres and balls. Other contributed papers address a variety of current topics in approximation theory, including eigenvalue sequences of positive integral operators, image registration, and support vector machines. This book will be of interest to mathematicians, engineers, and computer scientists working in approximation theory, computer-aided geometric design, numerical analysis, and related approximation areas.

  18. International Conference on Advanced Computing

    CERN Document Server

    Patnaik, Srikanta

    2014-01-01

    This book is composed of the Proceedings of the International Conference on Advanced Computing, Networking, and Informatics (ICACNI 2013), held at Central Institute of Technology, Raipur, Chhattisgarh, India during June 14–16, 2013. The book records current research articles in the domain of computing, networking, and informatics. The book presents original research articles, case-studies, as well as review articles in the said field of study with emphasis on their implementation and practical application. Researchers, academicians, practitioners, and industry policy makers around the globe have contributed towards formation of this book with their valuable research submissions.

  19. Conference

    CERN Multimedia

    Association du personnel

    2013-01-01

    A new integrated model for dementia management A Greek interdisciplinary scientific group proposes new technologies, novel neuroscientific concepts and advanced socio-economic and administrational organisation to ease the management of dementia in elderly Dr. Kostas Petsanis Thursday 23 May from 6.30 p.m. to 8.00 p.m. Council Chamber The general economic crisis while being a nightmare for the society, it has also given way to a new kind of reorganization and living. Elderly people come up against many health problems. A society of elders needs flexible solutions in every day living in order to avoid social and health stalemates. The mood and cognition in the elderly turn out to be doubtful and problematic. These situations give rise to a continuously growing number of depressed and demented elderly people with catastrophic results to the quality of life and social management. Αn interdisciplinary group of greek scientists proposes a novel advanced process of ambient interaction for elderly peo...

  20. Conference

    CERN Multimedia

    Staff Association

    2011-01-01

    Du Big Bang à l'Homme : la thermodynamique de l'évolution François Roddier Mercredi 16 novembre de 18 h 30 à 20 h 00 Salle du Conseil   En déséquilibre thermodynamique, l’univers ne cesse d’évoluer. Des structures de plus en plus complexes s’auto-organisent de façon à augmenter constamment la vitesse à laquelle l’énergie se dissipe. Ces structures se font, se défont et se refont, tout en mémorisant toujours plus d’information. Ce mécanisme s'applique aussi bien au Big Bang qu'à l'évolution darwinienne des espèces ou à l'auto-organisation des sociétés humaines. Il offre une base scientifique permettant de réfléchir à l'avenir de l'...

  1. 2010 Ceramics, Solid State Studies in Gordon Research Conference

    Energy Technology Data Exchange (ETDEWEB)

    John Halloran

    2010-08-20

    The 2010 Gordon Conference on Solid State Studies in Ceramics will present forefront research on ceramic materials in energy conversion, storage, and environmental sustainability. Oxide materials in advanced Li-ion batteries will be featured, including first principles computational methods, new experimental methods, novel synthesis, and the design of batteries that exploit nanoscale cathode materials. Several speakers address advances in oxides for solar applications, including photo-catalysts for solar hydrogen production and dye sensitized solar cells, along with thin film photovoltaics. Fast ionic conducting ceramics in electrochemical energy conversion and storage will be addressed for fuel cells and electrochemical storage. New concepts for electrochemical capacitor materials will be addressed, as will thermoelectric, geopolymers, and ceramics in nuclear energy. The Conference will bring together investigators at the forefront of their field as well as junior scientists in a collegial atmosphere, with programmed discussion sessions and informal gatherings in the afternoons and evenings. Poster presentations provide opportunities for junior scientists and graduate students to present their work and exchange ideas with leaders in the field. This Conference provides an avenue for scientists from different disciplines to explore new ideas and promotes cross-disciplinary collaborations in the various research areas represented.

  2. Gluten Sensitivity.

    Science.gov (United States)

    Catassi, Carlo

    2015-01-01

    Non-celiac gluten sensitivity (NCGS) is a syndrome characterized by intestinal and extraintestinal symptoms related to the ingestion of gluten-containing food in subjects who are not affected by either celiac disease (CD) or wheat allergy (WA). The prevalence of NCGS is not clearly defined yet. Indirect evidence suggests that NCGS is slightly more common than CD, the latter affecting around 1% of the general population. NCGS has been mostly described in adults, particularly in females in the age group of 30-50 years; however, pediatric case series have also been reported. Since NCGS may be transient, gluten tolerance needs to be reassessed over time in patients with NCGS. NCGS is characterized by symptoms that usually occur soon after gluten ingestion, disappear with gluten withdrawal, and relapse following gluten challenge within hours/days. The 'classical' presentation of NCGS is a combination of irritable bowel syndrome-like symptoms, including abdominal pain, bloating, bowel habit abnormalities (either diarrhea or constipation), and systemic manifestations such as 'foggy mind', headache, fatigue, joint and muscle pain, leg or arm numbness, dermatitis (eczema or skin rash), depression, and anemia. In recent years, several studies explored the relationship between the ingestion of gluten-containing food and the appearance of neurological and psychiatric disorders/symptoms like ataxia, peripheral neuropathy, schizophrenia, autism, depression, anxiety, and hallucinations (so-called gluten psychosis). The diagnosis of NCGS should be considered in patients with persistent intestinal and/or extraintestinal complaints showing a normal result of the CD and WA serological markers on a gluten-containing diet, usually reporting worsening of symptoms after eating gluten-rich food. NCGS should not be an exclusion diagnosis only. Unfortunately, no biomarker is sensitive and specific enough for diagnostic purposes; therefore, the diagnosis of NCGS is currently based on

  3. Use of the Novel INNO-LiPA Line Probe Assay for Detection of Hepatitis B Virus Variants That Confer Resistance to Entecavir Therapy▿

    Science.gov (United States)

    Jardi, Rosendo; Rodriguez-Frias, Francisco; Tabernero, David; Homs, Maria; Schaper, Melanie; Esteban, Rafael; Buti, Maria

    2009-01-01

    A line probe assay (INNO-LiPA DR, version 3) for the detection of hepatitis B virus mutations that confer resistance to entecavir therapy was evaluated. The INNO-LiPA DR assay is a highly sensitive assay that is easily applicable for the detection and monitoring of entecavir resistance-conferring mutations and is more sensitive than sequencing for the detection of mixed sequences. PMID:19052182

  4. 1st Iberian Robotics Conference

    CERN Document Server

    Sanfeliu, Alberto; Ferre, Manuel; ROBOT2013; Advances in robotics

    2014-01-01

    This book contains the proceedings of the ROBOT 2013: FIRST IBERIAN ROBOTICS CONFERENCE and it can be said that included both state of the art and more practical presentations dealing with implementation problems, support technologies and future applications. A growing interest in Assistive Robotics, Agricultural Robotics, Field Robotics, Grasping and Dexterous Manipulation, Humanoid Robots, Intelligent Systems and Robotics, Marine Robotics, has been demonstrated by the very relevant number of contributions. Moreover, ROBOT2013 incorporates a special session on Legal and Ethical Aspects in Robotics that is becoming a topic of key relevance. This Conference was held in Madrid (28-29 November 2013), organised by the Sociedad Española para la Investigación y Desarrollo en Robótica (SEIDROB) and by the Centre for Automation and Robotics - CAR (Universidad Politécnica de Madrid (UPM) and Consejo Superior de Investigaciones Científicas (CSIC)), along with the co-operation of Grupo Temático de Robótica CEA-GT...

  5. 2008 Gordon Research Conference on Electrodeposition [Conference summary report

    Energy Technology Data Exchange (ETDEWEB)

    Moffat, Thomas P.; Gray, Nancy Ryan

    2009-01-01

    Electrodeposition melds key aspects of electrochemistry and materials science. In the last decade the advent of a variety of remarkable in situ characterization methods combined with the ever expanding application of wet chemical processing in high end technological endeavors has transformed the nature of the field. The 'old black magic' is giving way to the rigors of science as the electrodeposition process plays a central role in the fabrication of state-of-the-art ULSI and MEMS devices as well as being a key tool in the fabrication of novel materials and nanostructures. This year the conference will consider several timely issues such as how electrodeposition can contribute to the effective production of energy conversion devices, ranging from solar collectors to fuel cell electrocatalysts. Likewise, the challenge of building contacts and interconnects for next generation electronics will be examined over length scales ranging from individual atoms or molecules to chip stacking. Electrochemical fabrication of magnetic materials and devices as well as composite materials will also be discussed. Nucleation and growth phenomena underlie all aspect of electrochemical deposition and this year's meeting will consider the effect of both adsorbates and stress state on morphological evolution during thin film growth. A variety of new measurement methods for studying the growing electrode/electrolyte interface will also be detailed. In addition to the scheduled talks a session of short talks on late breaking news will be held Wednesday evening. There will also be at least two lively poster sessions that are essential elements of the conference and to which all attendees are encouraged to contribute. This will be 7th Electrodeposition GRC and based on past experience it is the premier 'mixing bowl' where young investigators and international experts have an extended opportunity to interact in a fun and collegial atmosphere. The afternoons provide

  6. SmbFT, a putative ABC transporter complex, confers protection against the lantibiotic Smb in Streptococci.

    Science.gov (United States)

    Biswas, Saswati; Biswas, Indranil

    2013-12-01

    Streptococcus mutans, a dental pathogen, secretes different kinds of lantibiotic and nonlantibiotic bacteriocins. For self-protection, a bacteriocin producer strain must possess one or more cognate immunity mechanisms. We report here the identification of one such immunity complex in S. mutans strain GS-5 that confers protection against Smb, a two-component lantibiotic. The immunity complex that we identified is an ABC transporter composed of two proteins: SmbF (the ATPase component) and SmbT (the permease component). Both of the protein-encoding genes are located within the smb locus. We show that GS-5 becomes sensitized to Smb upon deletion of smbT, which makes the ABC transporter nonfunctional. To establish the role SmbFT in providing immunity, we heterologously expressed this ABC transporter complex in four different sensitive streptococcal species and demonstrated that it can confer resistance against Smb. To explore the specificity of SmbFT in conferring resistance, we tested mutacin IV (a nonlantibiotic), nisin (a single peptide lantibiotics), and three peptide antibiotics (bacitracin, polymyxin B, and vancomycin). We found that SmbFT does not recognize these structurally different peptides. We then tested whether SmbFT can confer protection against haloduracin, another two-component lantibiotic that is structurally similar to Smb; SmbFT indeed conferred protection against haloduracin. SmbFT can also confer protection against an uncharacterized but structurally similar lantibiotic produced by Streptococcus gallolyticus. Our data suggest that SmbFT truly displays immunity function and confer protection against Smb and structurally similar lantibiotics.

  7. PREFACE: International Nuclear Physics Conference 2010 (INPC2010)

    Science.gov (United States)

    Dilling, Jens

    2011-09-01

    Symmetries session is always one of the conference highlights. There, progress on Standard Model tests employing atomic nuclei or nuclear physics methods - which are used to probe complimentary sectors to large particle physics experiments, for example atomic and neutron EDM experiments - is reported. Recent progress was reported in the sector of nuclear beta decay as related to the testing of the CKM unitarity matrix, as well as the W-mass and the Weak Mixing Angle. The muon anomalous magnetic moment and its sensitivity for probing new physics and future experimental improvements are anticipated and showcase the activity in the field. The large oral and poster presentation program was extended to include special presentations by the IUPAP young scientist award winners. This prize is given out in the field of nuclear physics every three years during the INPC conference, and this year's winners were: Kenji Fukushima (Yukawa Institute for Theoretical Physics, Kyoto University), Peter Mueller (Argonne National Laboratory), and Lijuan Ruan (Brookhaven National Laboratory). These three scientists represent future excellence in nuclear physics in the fields of theoretical QCD, experimental techniques related to quark gluon plasma, and precision experiments in low energy nuclear halo physics. One keenly anticipated presentation, 'The Lamb shift in muonic hydrogen experiment', presented the results of the measurement of the proton rms charge radius. These results claimed a 5 sigma deviation from the established CODATA-value and in the future more tests will be needed to verify these findings. INPC 2010 made a special effort to attract many graduate students and post-doctoral fellows to the conference. This was achieved by a number of efforts, for example, TRIUMF combined its traditional summer school with the US National Science Foundation summer school for nuclear physics, and offered the school directly prior to the conference. This allowed the school to recruit some of the INPC

  8. Conference Support, 23rd Western Photosynthesis Conference 2014, Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Wachter, Rebekka [Arizona State Univ., Tempe, AZ (United States)

    2015-01-12

    The Western Photosynthesis Conference is a regional conference that is held on an annual basis to bring together researchers primarily from the Western United States to share their newest research advances on photosynthetic processes. The 23rd conference was focused on both fundamental and more applied research on the biological conversion of solar energy to various energy storage forms. Several particular areas of solar energy conversion were emphasized in this conference (see below). Some of these topics, such as carbon limitations on photosynthesis, biomimicry and phenotyping, have traditionally not been incorporated extensively in the Western Photosynthesis Conference. We found that these topics have substantially broadened of the scope of this meeting.

  9. Conference report: 12th Annual University of Wisconsin Land O'Lakes Bioanalytical Conference.

    Science.gov (United States)

    DeMuth, James E; Briscoe, Chad; Amaravadi, Lakshmi; Arnold, Mark E; Clement, Robert P; Fluhler, Eric N; Ji, Qin C; Stubbs, R John

    2011-10-01

    This University of Wisconsin School of Pharmacy bioanalytical conference is presented each year by the Extension Services in Pharmacy, the professional development department within the school. The purpose of this 4-day conference is to provide an educational forum to discuss issues and applications associated with the analysis of xenobiotics, metabolites, biologics and biomarkers in biological matrices. The conference is designed to include and encourage an open exchange of scientific and methodological applications for bioanalysis. To increase the interactive nature of the conference, the program was a mixture of lectures, poster sessions, round table discussions and workshops. This article summarizes the presentations at the 12th Annual Conference.

  10. Proceedings of the Scientific Conference on Energy and IT

    Energy Technology Data Exchange (ETDEWEB)

    Dahlquist, E.; Palm, J. (eds.)

    2009-07-01

    In connection with the Swedish conference and exhibition 'Energiting 2009' an international conference was held centered on energy efficiency, energy conservation and biofuels in households and industry. 17 contributions were indexed separately from this conference

  11. The end of an era? Midwifery conferences

    DEFF Research Database (Denmark)

    Vilain, Annette Dalsgaard; Stewart, Sarah

    2012-01-01

    such as geographical isolation, time and financial constraints restrict midwives' ability to attend conferences (McIntosh 2007; Patterson and Davis 2007). At the same time, the effectiveness of conferences for CPD has been questioned (Guskey 2000). In these days of financial retrenchment, CPD has to be innovative...

  12. Physics Conference TIM-15-16

    CERN Document Server

    2016-01-01

    The Conference is organized by the West University of Timisoara, Faculty of Physics. The scientific program of the conference will include invited lectures, oral and poster presentations, as well as discussions on various topics of present interest, such as, but not limited to condensed matter physics and applications, theoretical and computational physics, and applied physics.

  13. Conference Review - Languages and the Media

    DEFF Research Database (Denmark)

    Christoffersen, Ellen

    2007-01-01

    The main focus at the conference was accesibility i.e. making films, TV programs and video games accesible to people with hearing and visual disabilities.......The main focus at the conference was accesibility i.e. making films, TV programs and video games accesible to people with hearing and visual disabilities....

  14. International Conference on Vehicle and Automotive Engineering

    CERN Document Server

    Bolló, Betti

    2017-01-01

    This book presents the proceedings of the first vehicle engineering and vehicle industry conference. It captures the outcome of theoretical and practical studies as well as the future development trends in a wide field of automotive research. The themes of the conference include design, manufacturing, economic and educational topics.

  15. Innovative Conference Curriculum: Maximizing Learning and Professionalism

    Science.gov (United States)

    Hyland, Nancy; Kranzow, Jeannine

    2012-01-01

    This action research study evaluated the potential of an innovative curriculum to move 73 graduate students toward professional development. The curriculum was grounded in the professional conference and utilized the motivation and expertise of conference presenters. This innovation required students to be more independent, act as a critical…

  16. The Tenth National Dissemination Conference. A Report.

    Science.gov (United States)

    Herlig, Richard K.

    This report on the Tenth National Dissemination Conference of 1977 outlines the purposes of the conference and summarizes the present state of educational information dissemination. National programs are described, including the National Institute of Education State Capacity Building Program, the National Diffusion Network, the Dissemination…

  17. China International Cotton Conference Concluded in Xinjiang

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The 2007 China International Cotton Conference was held on June 27-29 in Urumqi,Xinjiang Municipality, China.With the theme"China’s Cotton Industry on WTO and It’s Implications The Global Market".the Conference proceeded with three main sessions,one focusing on the

  18. 78 FR 38311 - Reliability Technical Conference Agenda

    Science.gov (United States)

    2013-06-26

    ..., Fix, Track, and Report program enhanced reliability? b. What is the status of the NERC Reliability... Energy Regulatory Commission Reliability Technical Conference Agenda Reliability Technical Docket No. AD13-6-000 Conference. North American Electric Docket No. RC11-6-004 Reliability Corporation....

  19. Space Law Conference 2004 Held in Beijing

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    pace Law Conference 2004 Iointly organized by International Institute of Space Law (IISL) and China Institute of Space Law (CISL) was held in Beijing, China on 26427 April 2004. The purpose of this conference is to increase knowledge and expertise relating to space law in China and the

  20. 20 CFR 901.34 - Conferences.

    Science.gov (United States)

    2010-04-01

    ...' Benefits JOINT BOARD FOR THE ENROLLMENT OF ACTUARIES REGULATIONS GOVERNING THE PERFORMANCE OF ACTUARIAL... § 901.34 Conferences. (a) In general. The Executive Director may confer with an enrolled actuary... of the actuary or the Executive Director. (b) Voluntary suspension or termination of enrollment....

  1. International Conference on Microelectronics, Electromagnetics and Telecommunications

    CERN Document Server

    Rao, N; Kumar, S; Raj, C; Rao, V; Sarma, G

    2016-01-01

    This volume contains 73 papers presented at ICMEET 2015: International Conference on Microelectronics, Electromagnetics and Telecommunications. The conference was held during 18 – 19 December, 2015 at Department of Electronics and Communication Engineering, GITAM Institute of Technology, GITAM University, Visakhapatnam, INDIA. This volume contains papers mainly focused on Antennas, Electromagnetics, Telecommunication Engineering and Low Power VLSI Design.

  2. Reviewing the Electronic Resources & Libraries Conference

    Science.gov (United States)

    Tijerina, Bonnie

    2008-01-01

    The third Electronic Resources & Libraries (ER&L) conference gathered at Georgia Institute of Technology's Global Learning and Conference Center in Atlanta, Georgia, March 18-21, 2008. Over 360 attendees, from six countries and from 80% of the United States, represented their libraries and organizations resulting in a diverse and…

  3. Proceedings of the International Miconia Conference

    Science.gov (United States)

    Loope, L.L.; Meyer, J.-Y.; Hardesty, B. D.; Smith, C.W.

    2015-01-01

    This proceedings is a compilation of 15 of the 27 papers and posters that were presented at the 2009 International Miconia Conference. The Conference was held in Keanae Hawaii May 4th to 7th 2009, hosted by the Maui Invasive Species Committee. *No official abstract was available for the proceedings...K. Keck

  4. Highlights of 41st IATEFL Conference (Ⅱ)

    Institute of Scientific and Technical Information of China (English)

    Rathi Manohar

    2007-01-01

    @@ For this year's 41 st International Annual IATEFL Conference in Aberdeen,IATEFL and the British Council worked together to enable teacher audiences worldwide to participate in this year's IATEFL Conference via the interact and get a taste of exactly what happens over four exciting days each year around Easter.

  5. 21 CFR 514.5 - Presubmission conferences.

    Science.gov (United States)

    2010-04-01

    ... conferences prior to the submission of an NADA, supplemental NADA, or an ANADA to reach an agreement... conferences at any time prior to the filing of a NADA, supplemental NADA, or an ANADA. A request for a... divisions with respect to requirements for the investigation of new animal drugs and for NADAs,...

  6. Lift conference | 5-7 February

    CERN Multimedia

    2014-01-01

    Since 2006, Lift Events explore the business and social implications of new technologies through the organisation of international event series and open innovation programs in Europe, Asia and America. The next conference will be held on 5-7 February in Geneva.   (Image: © Lift Conference) The Lift Conference is one of the leading conferences on innovation in Europe and a key annual meeting for individuals and organizations wishing to understand and anticipate trends and innovation. Held every year in February in Geneva (5-7 February 2014), the Lift Conference is a three-day event consisting of talks, interactive workshops, exhibitions, and discussions bringing together over 1’000 participants from all society’s sectors and industries in a dynamic and informal environment with the aim to learn, connect, share and leverage innovation opportunities.   Extraordinary speakers will take to the stage at Lift14: Porter Erisman, former VP of Alibaba.com turned...

  7. Conference on Transportation and Urban Life

    CERN Document Server

    Wenzel, H

    1976-01-01

    All the papers in this volume were presented at a conference on Transportation and Urban Life, held in Munich during the third week of September, 1975. The conference was sponsored by the Special Programme Panels on Systems Science and Human Factors of the Science Committee of the North Atlantic Treaty Organisation. The distinguishing characteristic of the conference and of this volume lies in the combination of systems science and human factors contributions in the field of urban transportation. The initiative for attempting such a synthesis came from the sponsors. It is increasingly realised that the complexity of contemporary problems which applied scientists are being asked to solve is such that the coordinated efforts of several disciplines are needed to solve them. The brief which we formulated for the conference and distribu­ ted in our international call for papers was as follows: "The conference is intended to highlight significant psycho­ logical, SOCiological and economic aspects of transportatio...

  8. XIV International Conference on Mathematical Programming

    CERN Document Server

    Pardalos, Panos; Rapcsák, Tamás

    2001-01-01

    This volume contains refereed papers based on the lectures presented at the XIV International Conference on Mathematical Programming held at Matrahaza, Hungary, between 27-31 March 1999. This conference was organized by the Laboratory of Operations Research and Deci­ sion Systems at the Computer and Automation Institute, Hungarian Academy of Sciences. The editors hope this volume will contribute to the theory and applications of mathematical programming. As a tradition of these events, the main purpose of the confer­ ence was to review and discuss recent advances and promising research trends concerning theory, algorithms and applications in different fields of Optimization Theory and related areas such as Convex Analysis, Complementarity Systems and Variational Inequalities. The conference is traditionally held in the Matra Mountains, and housed by the resort house of the Hungarian Academy of Sciences. This was the 14th event of the long lasting series of conferences started in 1973. The organizers wish to...

  9. Topics from Australian Conferences on Teaching Statistics

    CERN Document Server

    Phillips, Brian; Martin, Michael

    2014-01-01

    The first OZCOTS conference in 1998 was inspired by papers contributed by Australians to the 5th International Conference on Teaching Statistics. In 2008, as part of the program of one of the first National Senior Teaching Fellowships, the 6th OZCOTS was held in conjunction with the Australian Statistical Conference, with Fellowship keynotes and contributed papers, optional refereeing and proceedings. This venture was so successful that the 7th and 8th OZCOTS were similarly run, conjoined with Australian Statistical Conferences in 2010 and 2012. Authors of papers from these OZCOTS conferences were invited to develop chapters for refereeing and inclusion in this volume. There are sections on keynote topics, undergraduate curriculum and learning, professional development, postgraduate learning, and papers from OZCOTS 2012. Because OZCOTS aim to unite statisticians and statistics educators, the approaches this volume takes are immediately relevant to all who have a vested interest in good teaching practices. Glo...

  10. Visual Information Communications International Conference

    CERN Document Server

    Nguyen, Quang Vinh; Zhang, Kang; VINCI'09

    2010-01-01

    Visual Information Communication is based on VINCI'09, The Visual Information Communications International Conference, September 2009 in Sydney, Australia. Topics covered include The Arts of Visual Layout, Presentation & Exploration, The Design of Visual Attributes, Symbols & Languages, Methods for Visual Analytics and Knowledge Discovery, Systems, Interfaces and Applications of Visualization, Methods for Multimedia Data Recognition & Processing. This cutting-edge book addresses the issues of knowledge discovery, end-user programming, modeling, rapid systems prototyping, education, and design activities. Visual Information Communications is an edited volume whose contributors include well-established researchers worldwide, from diverse disciplines including architects, artists, engineers, and scientists. Visual Information Communication is designed for a professional audience composed of practitioners and researchers working in the field of digital design and visual communications. This volume i...

  11. 10th International Conference Mechatronics

    CERN Document Server

    Jabloński, Ryszard

    2014-01-01

    Mechatronics, as the integrating framework of mechanical engineering, electrical engineering, computer technology, control engineering and automation forms a crucial part in the design, manufacture and maintenance of a wide range of engineering products and processes. The mechatronics itself changes rapidly in last decade, from original mixture of subfields into original approach in engineering as a technical discipline. The book you are holding is aimed to help the reader to orient in this evolving field of science and technology. „Mechatronics 2013: Recent Technological and Scientific Advances“ is the fourth volume following the previous editions in 2007, 2009 and 2011, providing the comprehensive and accessible coverage of advances in mechatronics presented on the 10th International Conference Mechatronics 2013, hosted this year at the Brno University of Technology, Czech Republic. The contributions, that passed the thorough review process, give an insight into current trends in research and developmen...

  12. European Conference on Data Analysis

    CERN Document Server

    Krolak-Schwerdt, Sabine; Böhmer, Matthias; Data Science, Learning by Latent Structures, and Knowledge Discovery; ECDA 2013

    2015-01-01

    This volume comprises papers dedicated to data science and the extraction of knowledge from many types of data: structural, quantitative, or statistical approaches for the analysis of data; advances in classification, clustering, and pattern recognition methods; strategies for modeling complex data and mining large data sets; applications of advanced methods in specific domains of practice. The contributions offer interesting applications to various disciplines such as psychology, biology, medical and health sciences; economics, marketing, banking, and finance; engineering; geography and geology;  archeology, sociology, educational sciences, linguistics, and musicology; library science. The book contains the selected and peer-reviewed papers presented during the European Conference on Data Analysis (ECDA 2013) which was jointly held by the German Classification Society (GfKl) and the French-speaking Classification Society (SFC) in July 2013 at the University of Luxembourg.

  13. Econophys-Kolkata VI Conference

    CERN Document Server

    Chakrabarti, Bikas; Chakraborti, Anirban; Ghosh, Asim

    2013-01-01

    The primary goal of the book is to present the ideas and research findings of active researchers such as physicists, economists, mathematicians and financial engineers working in the field of “Econophysics,” who have undertaken the task of modeling and analyzing systemic risk, network dynamics and other topics. Of primary interest in these studies is the aspect of systemic risk, which has long been identified as a potential scenario in which financial institutions trigger a dangerous contagion mechanism, spreading from the financial economy to the real economy. This type of risk, long confined to the monetary market, has spread considerably in the recent past, culminating in the subprime crisis of 2008. As such, understanding and controlling systemic risk has become an extremely important societal and economic challenge. The Econophys-Kolkata VI conference proceedings are dedicated to addressing a number of key issues involved. Several leading researchers in these fields report on their recent work and al...

  14. International Conference Educational Robotics 2016

    CERN Document Server

    Moro, Michele; Menegatti, Emanuele

    2017-01-01

    This book includes papers presented at the International Conference “Educational Robotics 2016 (EDUROBOTICS)”, Athens, November 25, 2016. The papers build on constructivist and constructionist pedagogy and cover a variety of topics, including teacher education, design of educational robotics activities, didactical models, assessment methods, theater robotics, programming & making electronics with Snap4Arduino, the Duckietown project, robotics driven by tangible programming, Lego Mindstorms combined with App Inventor, the Orbital Education Platform, Anthropomorphic Robots and Human Meaning Makers in Education, and more. It provides researchers interested in educational robotics with the latest advances in the field with a focus on science, technology, engineering, arts and mathematics (STEAM) education. At the same time it offers teachers and educators from primary to secondary and tertiary education insights into how educational robotics can trigger the development of technological interest and 21st c...

  15. European Conference on Complex Systems

    CERN Document Server

    Pellegrini, Francesco; Caldarelli, Guido; Merelli, Emanuela

    2016-01-01

    This work contains a stringent selection of extended contributions presented at the meeting of 2014 and its satellite meetings, reflecting scope, diversity and richness of research areas in the field, both fundamental and applied. The ECCS meeting, held under the patronage of the Complex Systems Society, is an annual event that has become the leading European conference devoted to complexity science. It offers cutting edge research and unique opportunities to study novel scientific approaches in a multitude of application areas. ECCS'14, its eleventh occurrence, took place in Lucca, Italy. It gathered some 650 scholars representing a wide range of topics relating to complex systems research, with emphasis on interdisciplinary approaches. The editors are among the best specialists in the area. The book is of great interest to scientists, researchers and graduate students in complexity, complex systems and networks.

  16. 11th KES International Conference

    CERN Document Server

    Kusek, Mario; Chen-Burger, Yun-Heh; Howlett, Robert; Jain, Lakhmi

    2017-01-01

    This volume highlights new trends and challenges in research on agents and the new digital and knowledge economy, and includes 23 papers classified into the following categories: business process management, agent-based modeling and simulation, and anthropic-oriented computing. All papers were originally presented at the 11th International KES Conference on Agents and Multi-Agent Systems – Technologies and Applications (KES-AMSTA 2017) held June 21–23, 2017 in Vilamoura, Algarve, Portugal. Today’s economy is driven by technologies and knowledge. Digital technologies can free, shift and multiply choices, and often intrude on the territory of other industries by providing new ways of conducting business operations and creating value for customers and companies. The topics covered in this volume include software agents, multi-agent systems, agent modeling, mobile and cloud computing, big data analysis, business intelligence, artificial intelligence, social systems, computer embedded systems and nature insp...

  17. 2nd Historic Mortars Conference

    CERN Document Server

    Hughes, John; Groot, Caspar; Historic Mortars : Characterisation, Assessment and Repair

    2012-01-01

    This volume focuses on research and practical issues connected with mortars on historic structures. The book is divided into four sections: Characterisation of Historic Mortars, Repair Mortars and Design Issues, Experimental Research into Properties of Repair Mortars, and Assessment and Testing. The papers present the latest work of researchers in their field. The individual contributions were selected from the contributions to the 2nd Historic Mortars Conference, which took place in Prague, September, 22-24, 2010. All papers were reviewed and improved as necessary before publication. This peer review process by the editors resulted in the 34 individual contributions included in here. One extra paper reviewing and summarising State-of-the-Art knowledge covered by this publication was added as a starting and navigational point for the reader. The editors believe that having these papers in print is important and they hope that it will stimulate further research into historic mortars and related subjects. 

  18. Conference on Large Scale Optimization

    CERN Document Server

    Hearn, D; Pardalos, P

    1994-01-01

    On February 15-17, 1993, a conference on Large Scale Optimization, hosted by the Center for Applied Optimization, was held at the University of Florida. The con­ ference was supported by the National Science Foundation, the U. S. Army Research Office, and the University of Florida, with endorsements from SIAM, MPS, ORSA and IMACS. Forty one invited speakers presented papers on mathematical program­ ming and optimal control topics with an emphasis on algorithm development, real world applications and numerical results. Participants from Canada, Japan, Sweden, The Netherlands, Germany, Belgium, Greece, and Denmark gave the meeting an important international component. At­ tendees also included representatives from IBM, American Airlines, US Air, United Parcel Serice, AT & T Bell Labs, Thinking Machines, Army High Performance Com­ puting Research Center, and Argonne National Laboratory. In addition, the NSF sponsored attendance of thirteen graduate students from universities in the United States and abro...

  19. 11th International Conference Mechatronics

    CERN Document Server

    Brezina, Tomas

    2016-01-01

    Focusing on the most rapidly changing areas of mechatronics, this book discusses signals and system control, mechatronic products, metrology and nanometrology, automatic control & robotics, biomedical engineering, photonics, design manufacturing and testing of MEMS. It is reflected in the list of contributors, including an international group of 302 leading researchers representing 12 countries. The book is intended for use in academic, government and industry R&D departments, as an indispensable reference tool for the years to come. Thid volume can serve a global community as the definitive reference source in Mechatronics. The book comprises carefully selected 93 contributions presented at the 11th International Conference Mechatronics 2015, organized by Faculty of Mechatronics, Warsaw University of Technology, on September 21-23, in Warsaw, Poland. .

  20. Conference Trends in Logic XI

    CERN Document Server

    Wansing, Heinrich; Willkommen, Caroline; Recent Trends in Philosophical Logic

    2014-01-01

    This volume presents recent advances in philosophical logic with chapters focusing on non-classical logics, including paraconsistent logics, substructural logics, modal logics of agency and other modal logics. The authors cover themes such as the knowability paradox, tableaux and sequent calculi, natural deduction, definite descriptions, identity, truth, dialetheism, and possible worlds semantics.   The developments presented here focus on challenging problems in the specification of fundamental philosophical notions, as well as presenting new techniques and tools, thereby contributing to the development of the field. Each chapter contains a bibliography, to assist the reader in making connections in the specific areas covered. Thus this work provides both a starting point for further investigations into philosophical logic and an update on advances, techniques and applications in a dynamic field.   The chapters originate from papers presented during the Trends in Logic XI conference at the Ruhr University ...

  1. ICRESH-ARMS 2015 Conference

    CERN Document Server

    Ahmadi, Alireza; Verma, Ajit; Varde, Prabhakar

    2016-01-01

    Containing selected papers from the ICRESH-ARMS 2015 conference in Lulea, Sweden, collected by editors with years of experiences in Reliability and maintenance modeling, risk assessment, and asset management, this work maximizes reader insights into the current trends in Reliability, Availability, Maintainability and Safety (RAMS) and Risk Management. Featuring a comprehensive analysis of the significance of the role of RAMS and Risk Management in the decision making process during the various phases of design, operation, maintenance, asset management and productivity in Industrial domains, these proceedings discuss key issues and challenges in the operation, maintenance and risk management of complex engineering systems and will serve as a valuable resource for those in the field.

  2. 2013 GASEOUS IONS GORDON RESEARCH CONFERENCE, FEBRUARY 24 - MARCH 1, 2013

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Evan

    2013-03-01

    The Gaseous Ions: Structures, Energetics and Reactions Gordon Research Conference will focus on ions and their interactions with molecules, surfaces, electrons, and light. The long-standing goal of our community is to develop new strategies for capturing complex molecular architectures as gas phase ions where they can be isolated, characterized and manipulated with great sensitivity. Emergent areas of interest include catalytic mechanisms, cryogenic processing of ions extracted from solution, ion fragmentation mechanisms, and new methods for ion formation and structural characterization. The conference will cover theoretical and experimental advances on systems ranging from model studies at the molecular scale to preparation of nanomaterials and characterization of large biological molecules.

  3. 4th Neutron Transmutation Doping Conference

    CERN Document Server

    1984-01-01

    viii The growing use of NTD silicon outside the U. S. A. motivated an interest in having the next NTD conference in Europe. Therefore, the Third International Conference on Neutron Transmutation-Doped Silicon was organized by Jens Guldberg and held in Copenhagen, Denmark on August 27-29, 1980. The papers presented at this conference reviewed the developments which occurred during the t'A'O years since the previous conference and included papers on irradiation technology, radiation-induced defects, characteriza­ tion of NTD silicon, and the use of NTD silicon for device appli­ cations. The proceedings of this conference were edited by Jens Guldberg and published by Plenum Press in 1981. Interest in, and commercial use of, NTD silicon continued to grow after the Third NTD Conference, and research into neutron trans­ mutation doping of nonsilicon semiconductors had begun to accel­ erate. The Fourth International Transmutation Doping Conference reported in this volume includes invited papers summarizing the p...

  4. PREFACE: International Conference on Electrical Bioimpedance

    Science.gov (United States)

    Sadleir, Rosalind; Woo, Eung Je

    2010-04-01

    logo The XIVth International Conference on Electrical Bioimpedance, held in conjunction with the 11th Conference on Biomedical Applications of EIT (ICEBI & EIT 2010), took place from 4-8 April 2010 in the Reitz Union of the University of Florida, in Gainesville, USA. This was the first time since its inception in 1969 that the ICEBI was held in the United States. As in the last three conferences (Graz 2007, Gdansk 2004 and Oslo 2001) the ICEBI was combined with the Conference on Biomedical Applications of EIT - a mutually beneficial approach for those interested in the biophysics of tissue electrical properties and those developing imaging methods and measurement systems based thereon. This year's conference was particularly notable for the many papers presented on hybrid and emerging imaging techniques such as Electric Property Tomography (EPT), Magneto Acoustic Tomography using Magnetic Induction (MAT-MI) and Magnetic Resonance Electrical Impedance Tomography (MREIT); sessions on Cell Scale Impedance, Cardiac Impedance and Imaging Neural Activity. About 180 scientists from all over the world attended, including keynote speakers on topics of fundamental electromagnetic principles (Jaakko Malmivuo), Electrical Source and Impedance Imaging (Bin He), Bioimpedance applications in Nephrology, (Nathan Levin), and Lung EIT (Gerhard Wolf). The papers in this volume are peer-reviewed four-page works selected from over 150 presented in oral and poster sessions at the conference. The complete program is available from the conference website.

  5. The Institute of Archaeology Conference Competition

    Directory of Open Access Journals (Sweden)

    Ruth D Whitehouse

    2014-10-01

    Full Text Available One of the most creative innovations of Stephen Shennan’s directorship is the annual Institute of Archaeology Conference Competition. This scheme is now in its seventh year (with the result of the eighth competition just announced and it seems a good time to document and celebrate its success. The competition takes place in the spring of each year, with the winning conference being held in the following academic year. In the present format, applicants are asked to outline and justify the conference topic, list the proposed speakers and present a rough budget, with an indication of where the rest of the funds will be obtained if the conference costs are likely to exceed the £2000 of the award. There is no restriction as to the subject of the conference or its scale, though it is stipulated that the award must make a significant difference to the viability of the conference and that the conference fee for Institute attendees should be minimal, with a maximum of £10 for students.

  6. The Eighth Liquid Matter Conference.

    Science.gov (United States)

    Dellago, Christoph; Kahl, Gerhard; Likos, Christos N

    2012-07-18

    The Eighth Liquid Matter Conference (LMC8) was held at the Universität Wien from 6-10 September 2011. Initiated in 1990, the conferences of this series cover a broad range of highly interdisciplinary topics, ranging from simple liquids to soft matter and biophysical systems. The vast spectrum of scientific subjects presented and discussed at the LMC8 is reflected in the themes of the ten symposia: Ionic and quantum liquids, liquid metals Water, solutions and reaction dynamics Liquid crystals Polymers, polyelectrolytes, biopolymers Colloids Films, foams, surfactants, emulsions, aerosols Confined fluids, interfacial phenomena Supercooled liquids, glasses, gels Non-equilibrium systems, rheology, nanofluids Biofluids, active matter This special issue contains scientific papers, authored by participants of the LMC8, which provide a cross-section of the scientific activities in current liquid matter science, as discussed at the conference, and demonstrate the scientific as well as methodological progress made in this field over the past couple of years. The Eighth Liquid Matter Conference contents The Eighth Liquid Matter ConferenceChristoph Dellago, Gerhard Kahl and Christos N Likos Comparing light-induced colloidal quasicrystals with different rotational symmetriesMichael Schmiedeberg and Holger Stark Hydrogen bond network relaxation in aqueous polyelectrolyte solutions: the effect of temperatureS Sarti, D Truzzolillo and F Bordi Equilibrium concentration profiles and sedimentation kinetics of colloidal gels under gravitational stressS Buzzaccaro, E Secchi, G Brambilla, R Piazza and L Cipelletti The capillary interaction between two vertical cylindersHimantha Cooray, Pietro Cicuta and Dominic Vella Hydrodynamic and viscoelastic effects in polymer diffusionJ Farago, H Meyer, J Baschnagel and A N Semenov A density-functional theory study of microphase formation in binary Gaussian mixturesM Carta, D Pini, A Parola and L Reatto Microcanonical determination of the

  7. 7th International Conference of Political Economy

    Directory of Open Access Journals (Sweden)

    Gülçin BEKEN

    2016-06-01

    Full Text Available Abstract. In this study, the 7th International Conference of Political Economy in İstanbul was evaluated. This year it  was organized by Marmara University Faculty of Economics and Batman University Faculty of Economics and Administrative Sciences with the cooperation of their international supporters all over the world. These conference series are said to be successful to bring all the scholars, academicians, students, and volunteers who are interested in political economy. Starting from 2009, the contribution of these conference series into the field of political economy is undeniable.Keywords. Political Economy, State, Taxation, Institutions, Poverty.JEL. F50, P16, H20, H70.

  8. International Conference on Theoretical and Computational Physics

    CERN Document Server

    2016-01-01

    Int'l Conference on Theoretical and Computational Physics (TCP 2016) will be held from August 24 to 26, 2016 in Xi'an, China. This Conference will cover issues on Theoretical and Computational Physics. It dedicates to creating a stage for exchanging the latest research results and sharing the advanced research methods. TCP 2016 will be an important platform for inspiring international and interdisciplinary exchange at the forefront of Theoretical and Computational Physics. The Conference will bring together researchers, engineers, technicians and academicians from all over the world, and we cordially invite you to take this opportunity to join us for academic exchange and visit the ancient city of Xi’an.

  9. 6th iTi Conference

    CERN Document Server

    Kampers, Gerrit; Oberlack, Martin; Wacławczyk, Marta; Talamelli, Alessandro

    2016-01-01

    This volume collects the edited and reviewed contributions presented in the 6th iTi Conference in Bertinoro, covering fundamental and applied aspects in turbulence. In the spirit of the iTi conference, the volume has been produced after the conference so that the authors had the possibility to incorporate comments and discussions raised during the meeting. In the present book the contributions have been structured according to the topics : I Theory II Wall bounded flows III Particles in flows IV Free flows V Complex flows The volume is dedicated to the memory of Prof. Konrad Bajer who prematurely passed away in Warsaw on August 29, 2014. .

  10. The Roles of Conference Papers in IS

    DEFF Research Database (Denmark)

    Lanamäki, Arto; Persson, John Stouby

    2016-01-01

    understanding of conference papers in IS and the role they play for the authoring researchers. We present the first analysis of the papers published during the first six years (2010-2015) in the Scandinavian Conference on Information Systems (SCIS). We conducted interviews with ten SCIS authors. Following...... a framework adopted from Åkerlind [1], we identified how SCIS papers have the roles of fulfilling requirements, establishing oneself, developing personally, enabling change, and other roles. This article contributes to the reflection literature on the IS field by applying a practice lens to understand...... the role of conference papers in research....

  11. 4. International reservoir characterization technical conference

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-04-01

    This volume contains the Proceedings of the Fourth International Reservoir Characterization Technical Conference held March 2-4, 1997 in Houston, Texas. The theme for the conference was Advances in Reservoir Characterization for Effective Reservoir Management. On March 2, 1997, the DOE Class Workshop kicked off with tutorials by Dr. Steve Begg (BP Exploration) and Dr. Ganesh Thakur (Chevron). Tutorial presentations are not included in these Proceedings but may be available from the authors. The conference consisted of the following topics: data acquisition; reservoir modeling; scaling reservoir properties; and managing uncertainty. Selected papers have been processed separately for inclusion in the Energy Science and Technology database.

  12. 13th International Conference on Chlamydomonas

    Energy Technology Data Exchange (ETDEWEB)

    Silflow, Carolyn D. [University of Minnesota

    2014-03-11

    The 13th International Conference on Chlamydomonas (EMBO Workshop on the Cell and Molecular Biology of Chlamydomonas) was held May 27 to June 1, 2008 in Hyeres, France. The conference was the biennial meeting for all researchers studying the green algal systems Chlamydomonas and Volvox. The conference brought together approximately 200 investigators from around the world (North America, Asia, Europe and Australia) representing different fields and disciplines (cell biology, genetics, biochemistry, biophysics, plant physiology, genomics). It provided an opportunity for investigators from different countries to share methodologies and to discuss recent results with a focus on the Chlamydomonas experimental system.

  13. 22nd International Conference on Soft Computing

    CERN Document Server

    2017-01-01

    This proceeding book contains a collection of selected accepted papers of the Mendel conference held in Brno, Czech Republic in June 2016. The proceedings book contains three chapters which present recent advances in soft computing including intelligent image processing. The Mendel conference was established in 1995 and is named after the scientist and Augustinian priest Gregor J. Mendel who discovered the famous Laws of Heredity. The main aim of the conference is to create a regular possibility for students, academics and researchers to exchange ideas and novel research methods on a yearly basis.

  14. Coaltrans 2003 Vienna. Conference documentation and information

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    The sessions of the conference are: overview of the international coal markets; coal's role in changing European market; overview of the shipping markets; coking coal in 2004 and beyond; risk management and hedging; steam coal forecast; and coal in a carbon constrained future. The conference documentation mainly includes the text of overheads/slides of most but not all of the papers. Six papers have been abstracted separately for the Coal Abstracts database. The complete set of papers is available, to conference delegates, on the website www.coaltrans.com.

  15. Fifth French-German Conference on Optimization

    CERN Document Server

    1989-01-01

    The 2-yearly French-German Conferences on Optimization review the state-of-the-art and the trends in the field. The proceedings of the Fifth Conference include papers on projective methods in linear programming (special session at the conference), nonsmooth optimization, two-level optimization, multiobjective optimization, partial inverse method, variational convergence, Newton type algorithms and flows and on practical applications of optimization. A. Ioffe and J.-Ph. Vial have contributed survey papers on, respectively second order optimality conditions and projective methods in linear programming.

  16. SIAM conference on applications of dynamical systems

    Energy Technology Data Exchange (ETDEWEB)

    1992-01-01

    A conference (Oct.15--19, 1992, Snowbird, Utah; sponsored by SIAM (Society for Industrial and Applied Mathematics) Activity Group on Dynamical Systems) was held that highlighted recent developments in applied dynamical systems. The main lectures and minisymposia covered theory about chaotic motion, applications in high energy physics and heart fibrillations, turbulent motion, Henon map and attractor, integrable problems in classical physics, pattern formation in chemical reactions, etc. The conference fostered an exchange between mathematicians working on theoretical issues of modern dynamical systems and applied scientists. This two-part document contains abstracts, conference program, and an author index.

  17. Gulf International Conference on Applied Mathematics 2013

    CERN Document Server

    Advances in Applied Mathematics

    2014-01-01

    This volume contains contributions from the Gulf International Conference in Applied Mathematics, held at the Gulf University for Science & Technology. The proceedings reflects the three major themes of the conference. The first of these was mathematical biology, including a keynote address by Professor Philip Maini. The second theme was computational science/numerical analysis, including a keynote address by Professor Grigorii Shishkin. The conference also addressed more general applications topics, with papers in business applications, fluid mechanics, optimization, scheduling problems, and engineering applications, as well as a keynote by Professor Ali Nayfeh.

  18. 76 FR 56770 - Food and Drug Administration/Xavier University Global Outsourcing Conference

    Science.gov (United States)

    2011-09-14

    ... Outsourcing Conference AGENCY: Food and Drug Administration, HHS. ACTION: Notice of public conference. SUMMARY..., is announcing a public conference entitled ``FDA/Xavier University Global Outsourcing Conference... industry experts. This conference drives collaboration on the topic of global outsourcing compliance...

  19. 77 FR 41416 - Food and Drug Administration/Xavier University Global Outsourcing Conference

    Science.gov (United States)

    2012-07-13

    ... Outsourcing Conference AGENCY: Food and Drug Administration, HHS. ACTION: Notice of public conference. SUMMARY..., is announcing a public conference entitled ``FDA/Xavier University Global Outsourcing Conference... experts. This conference drives collaboration on the topic of global outsourcing compliance by...

  20. Designing Scientific Academic Conferences as a Learning Environment: How to Stimulate Active Learning at Academic Conferences?

    DEFF Research Database (Denmark)

    Verbeke, Johan

    2015-01-01

    architecture, arts and design) and on the way knowledge sharing and knowledge development was stimulated at these events. These conferences included less traditional conference designs, collective learning and explicit sharing of understanding between participants. Results: Collaboration in small groups...... as well as moments of collective learning. Organizers are recommended to adopt an explicit conference design. Constructivist content: Following a constructivist approach to learning environments, this paper stresses the importance of scheduling moments of active and collective learning and knowledge...

  1. Mathematical Problems in Biology : Victoria Conference

    CERN Document Server

    1974-01-01

    A conference on "Some Mathematical Problems in Biology" was held at the University of Victoria, Victoria, B. C. , Canada, from May 7 - 10, 1973. The participants and invited speakers were mathematicians interested in problems of a biological nature, and scientists actively engaged in developing mathematical models in biological fields. One aim of the conference was to attempt to assess what the recent rapid growth of mathematical interaction with the biosciences has accomplished and may accomplish in the near future. The conference also aimed to expose the problems of communication bet~",een mathematicians and biological scientists, and in doing so to stimulate the interchange of ideas. It was recognised that the topic spans an enormous breadth, and little attempt was made to balance the very diverse areas. Widespread active interest was shown in the conference, and just over one hundred people registered. The varied departments and institutions across North America from which the participants came made it bo...

  2. 2011 GASEOUS IONS GORDON RESEARCH CONFERENCE

    Energy Technology Data Exchange (ETDEWEB)

    Scott Anderson

    2011-03-04

    The Gaseous Ions: Structures, Energetics and Reactions Gordon Research Conference will focus on ions and their interactions with molecules, surfaces, electrons, and light. The conference will cover theory and experiments, and systems ranging from molecular to biological to clusters to materials. The meeting goal continues to be bringing together scientists interested in fundamentals, with those applying fundamental phenomena to a wide range of practical problems. Each of the ten conference sessions will focus on a topic within this spectrum, and there will also be poster sessions for contributed papers, with sufficient space and time to allow all participants to present their latest results. To encourage active participation by young investigators, about ten of the poster abstracts will be selected for 15 minute 'hot topic' talks during the conference sessions. Hot topic selection will be done about a month before the meeting. Funds should be available to offset the participation cost for young investigators.

  3. 36 CFR 1150.61 - Prehearing conference.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Prehearing conference. 1150.61 Section 1150.61 Parks, Forests, and Public Property ARCHITECTURAL AND TRANSPORTATION BARRIERS... a part of the record. ...

  4. Origin of the Caribbean Plate Conference

    Institute of Scientific and Technical Information of China (English)

    Keith H. James; Maria Antonieta Lorente

    2006-01-01

    @@ An international research conference, entitled "Geology of the area between North and South America, with focus on the origin of the Caribbean Plate", took place in Siguenza, Spain, from May 29-June 2, 2006.

  5. China International Cotton Conference Concluded in Xinjiang

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ The 2007 China International Cotton Conference was held on June 27-29 in Urumqi, Xinjiang Municipality, China. With the theme "China's Cotton Industry on WTO and It's Implications The Global Market".

  6. III Advanced Ceramics and Applications Conference

    CERN Document Server

    Gadow, Rainer; Mitic, Vojislav; Obradovic, Nina

    2016-01-01

    This is the Proceedings of III Advanced Ceramics and Applications conference, held in Belgrade, Serbia in 2014. It contains 25 papers on various subjects regarding preparation, characterization and application of advanced ceramic materials.

  7. Student Support for EIPBN 2010 Conference

    Energy Technology Data Exchange (ETDEWEB)

    Reginald C. Farrow

    2011-03-11

    The 54th International Conference on Electron, Ion and Photon Beam Technology and Nanofabrication, 2010, held at the Egan Convention Center and Hilton in Anchorage, Alaska, June 1 to 4, 2010 was a great success in large part because financial support allowed robust participation from students. The conference brought together 444 engineers and scientists from industries and universities from all over the world to discuss recent progress and future trends. Among the emerging technologies that are within the scope of EIPBN is Nanofabrication for Energy Sources along with nanofabrication for the realization of low power integrated circuits. Every year, EIPBN provides financial support for students to attend the conference.The students gave oral and poster presentations of their research and many published peer reviewed articles in a special conference issue of the Journal of Vacuum Science and Technology B. The Department of Energy Office of Basic Energy Sciences supported 20 students from US universities with a $15,000.

  8. European Conference on Molecular Biology EMBO

    CERN Multimedia

    1967-01-01

    European Conference on Molecular Biology, which eventually led to the setting up of EMBO, was held at CERN in April. Olivier Reverdin is adressing the delegates. Bernard Gregory is on the left and Willy Spuhler in the centre.

  9. Survivorship conference highlights research for survivor care

    Science.gov (United States)

    More than 400 leading experts in cancer survivorship convened today for a conference, Cancer Survivorship Research: Translating Science to Care, to focus on such current concerns as how obesity might not have the same effects on all cancer survivors, and

  10. 5th International Conference on Atmospheric Electricity

    CERN Document Server

    Reiter, Reinhold; Landsberg, Helmut

    1976-01-01

    These Proceedings are published to give a full account of the Fifth International Conference on Atmospheric Electricity held in September 1974 in Garmisch-Partenkirchen in the Bavarian Alps in Germany. Traditionally, the Proceedings of these Conferences have served as reference books updating the textbooks and monographs on Atmospheric Electricity. As treated by these Conferences, Atmos­ pheric Electricity covers all aspects of this science, including the processes and problems which reach out into the Earth's environment as well as analogous processes on other planets and on the Moon. A history of these Conferences, an account of their purpose, and an outline of the scope and the preparation is to be found at the end of these Proceedings. There, also the Business Meetings of the involved organizations are mentioned. The Proceedings closely follow the original program and are accordingly organized into "Sessions". The papers printed in each "Session" in this book are the ones which were accepted for the sess...

  11. PREFACE: 10th Joint Conference on Chemistry

    Science.gov (United States)

    2016-02-01

    The 10th Joint Conference on Chemistry is an international conference organized by 4 chemistry departments of 4 universities in central Java, Indonesia. The universities are Sebelas Maret University, Diponegoro University, Semarang State University and Soedirman University. The venue was at Solo, Indonesia, at September 8-9, 2015. The total conference participants are 133 including the invited speakers. The conference emphasized the multidisciplinary chemical issue and impact of today's sustainable chemistry which covering the following topics: • Material innovation for sustainable goals • Development of renewable and sustainable energy based on chemistry • New drug design, experimental and theoretical methods • Green synthesis and characterization of material (from molecule to functionalized materials) • Catalysis as core technology in industry • Natural product isolation and optimization

  12. 11th International Conference FQAS 2015

    CERN Document Server

    Christiansen, Henning; Kacprzyk, Janusz; Larsen, Henrik; Pasi, Gabriella; Pivert, Olivier; Tré, Guy; Vila, Maria; Yazici, Adnan; Zadrożny, Sławomir

    2016-01-01

    This volume contains the papers presented at the Eleventh Flexible Query Answering Systems 2015 (FQAS-2015) held on October 26-28, 2015 in Cracow, Poland. The international conferences on Flexible Query Answering Systems (FQAS) are a series of premier conferences focusing on the key issue in the information society of providing easy, flexible, and intuitive access to information and knowledge to everybody, even people with a very limited computer literacy. In targeting this issue, the Conference draws on several research areas, such as information retrieval, database management, information filtering, knowledge representation, soft computing, management of multimedia information, and human-computer interaction. The Conference provides a unique opportunity for researchers, developers and practitioners to explore new ideas and approaches in a multidisciplinary forum.  .

  13. The Impressive Annual Conference of ITMF

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Experiencing the last annual conference of ITMFin Mauritius,we attended this spectacle pageant thisyear in Shanghai.Based on the features of currentsituation in textile industry,ITMF this time chantedthe slogan

  14. Latin American Conference on Agricultural Education

    Science.gov (United States)

    Agan, Ray

    1971-01-01

    Presents the subject matter of a UNESCO sponsored conference in Pamplona, Colombia, April 26- May 23, 1970 of school directors and Ministry officials in Agricultural Education from 12 Latin American Countries. (GB)

  15. Ammi Canada 2015 Annual Conference: Abstract Titles

    Directory of Open Access Journals (Sweden)

    2015-01-01

    Full Text Available Abstracts to be presented at the 2015 Annual Conference of the Association of Medical Microbiology and Infectious Disease Canada, April 16 to 18, Charlottetown, Prince Edward Island, alphabetized according to the surname of the first author.

  16. Bandwidth challenge teams at SC2003 conference

    CERN Multimedia

    2003-01-01

    Results from the fourth annual High-Performance Bandwidth Challenge, held in conjunction with SC2003, the international conference on high-performance computing and networking which occurred last week in Phoenix, AZ (1 page).

  17. China-DPRK Friendship Cities Conference Held

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    <正>The China-DPRK Friendship Cities Conference cosponsored by the CPAFFC and the Committee for Cultural Relations with Foreign Countries of DPR Korea (CCRFCK) was held in Pyongyang on May 12. A 36-member delegation

  18. The fifth international conference on Arabidopsis research

    Energy Technology Data Exchange (ETDEWEB)

    Hangarter, R.; Scholl, R.; Davis, K.; Feldmann, K.

    1993-12-31

    This volume contains abstracts of oral and poster presentations made in conjunction with the Fifth International Conference on Arabidopsis Research held August 19--22, 1993 at the Ohio State University, Columbus, Ohio.

  19. Physics at the FQMT'11 conference

    NARCIS (Netherlands)

    Špička, V.; Nieuwenhuizen, T.M.; Keefe, P.D.

    2012-01-01

    This paper deals with the recent state of the art of the following topics presented at the FQMT’11 conference: foundations of quantum physics, quantum measurement; nonequilibrium quantum statistical physics; quantum thermodynamics; quantum measurement, entanglement and coherence; dissipation,

  20. Global Conference on Applied Physics and Mathematics

    CERN Document Server

    2016-01-01

    The Global Conference on Applied Physics and Mathematics is organized by academics and researchers belonging to different scientific areas of the C3i/Polytechnic Institute of Portalegre (Portugal) and the University of Extremadura (Spain) with the technical support of ScienceKnow Conferences. The event has the objective of creating an international forum for academics, researchers and scientists from worldwide to discuss worldwide results and proposals regarding to the soundest issues related to Applied Physics and Mathematics. This event will include the participation of renowned keynote speakers, oral presentations, posters sessions and technical conferences related to the topics dealt with in the Scientific Program as well as an attractive social and cultural program. The papers will be published in the Proceedings e-books. The proceedings of the conference will be sent to possible indexing on Thomson Reuters (selective by Thomson Reuters, not all-inclusive) and Google Scholar. Those communications con...

  1. Eighteenth annual West Coast theoretical chemistry conference

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-05-01

    Abstracts are presented from the eighteenth annual west coast theoretical chemistry conference. Topics include molecular simulations; quasiclassical simulations of reactions; photodissociation reactions; molecular dynamics;interface studies; electronic structure; and semiclassical methods of reactive systems.

  2. 19th Polish Control Conference

    CERN Document Server

    Kacprzyk, Janusz; Oprzędkiewicz, Krzysztof; Skruch, Paweł

    2017-01-01

    This volume contains the proceedings of the KKA 2017 – the 19th Polish Control Conference, organized by the Department of Automatics and Biomedical Engineering, AGH University of Science and Technology in Kraków, Poland on June 18–21, 2017, under the auspices of the Committee on Automatic Control and Robotics of the Polish Academy of Sciences, and the Commission for Engineering Sciences of the Polish Academy of Arts and Sciences. Part 1 deals with general issues of modeling and control, notably flow modeling and control, sliding mode, predictive, dual, etc. control. In turn, Part 2 focuses on optimization, estimation and prediction for control. Part 3 is concerned with autonomous vehicles, while Part 4 addresses applications. Part 5 discusses computer methods in control, and Part 6 examines fractional order calculus in the modeling and control of dynamic systems. Part 7 focuses on modern robotics. Part 8 deals with modeling and identification, while Part 9 deals with problems related to security, fault ...

  3. NSF-CBMS Regional Conference

    CERN Document Server

    Rubenfeld, Lester

    1981-01-01

    The articles of these proceedings arise from a NSF-CBMS regional conference on the mathematical modeling of the hearing process, that was held at Rensselaer Polytechnic Institute in the summer of 1980. To put the a=ticles in perspective, it is best to briefly review the history of suc~ modeling. It has proceeded, more or less, in three stages. The first was initiated by Herman Helmholtz in the 1880's, whose theories dominated the subject for years. However, because of his lack of accurate experimental data and his heuristic arguments it became apparent that his models needed revision. Accordingly, based on the experimental observations of von Bekesy, the "long wave" theories were developed in the 1950's by investigators such as Zwislocki, Peterson, and Bogert. However, as the ex?eri~ents became more refined (such as Rhode's ~wssbauer Measurements) even these models came into question. This has brought on a flurry of 'activity in recent years into how to extend the models to account for these more recent eXT. ...

  4. 3rd International EXAFS Conference

    CERN Document Server

    Hedman, Britt; Penner-Hahn, James

    1984-01-01

    This volume contains the Proceedings of the Third International EXAFS Conference, hosted by Stanford University and the Stanford Synchrotron Radiation Laboratory on July 16-20, 1984. The meeting, co-chaired by Professors Arthur Bienenstock and Keith Hodgson, was attended by over 200 scientists representing a wide range of scientific disciplines. The format of the meeting consisted of 51 invited presenta­ tions and four days of poster sessions. This Proceedings is a compilation of 139 contributions from both invited speakers and authors of contributed posters. The last ten years has seen the rapid maturation of x-ray absorption spectrosco­ pyas a scientific discipline. The vitality of the field is reflected in the diver­ sity of applications found in the Proceedings. Recent work continues to probe the limits of x-ray spectroscopy, with proven techniques being extended to, for examp­ le, very low or high energy studies, to very dilute systems, and to studies of surface structure. In fact, the title of the c...

  5. First Swiss building and urban simulation conference. Conference proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Zweifel, G.; Citherlet, S.; Afjei, T.; Pahud, D.; Robinson, D.; Schaelin, A.

    2010-07-01

    These contributions presented at a conference, held in 2009 in Horw, near Lucerne, Switzerland, deal with the simulation of building technical services. Three contribution blocks dealt with thermal and heating, ventilation and air-conditioning (HVAC) simulation, airflow and stochastic modelling and urban simulation. In the thermal and HVAC simulation session, the potential and limitations of building energy performance simulation is examined from an engineering perspective, a parametric study of an air heat exchanger for the cooling of buildings is presented and a comparison of measured and estimated electric energy use and the impact of assumed occupancy patterns is made. Contributions on standard solutions for energy efficient heating and cooling with heat pumps, the validation and certification of dynamic building simulation tools, standards and tools for the energy performance of buildings with a simple chiller model and the system-simulation of a central solar heating plant with seasonal duct storage in Geneva, Switzerland, are presented. In the airflow and stochastic modelling session, the optimisation of air flow in operating theatres is examined, and air-flow phenomena in flats are explained with illustrations of computational fluid dynamics (CFD). Also, the comparison of test reference years to stochastically generated time series and a comprehensive stochastic model of window usage are discussed. Contributions on the simulation of air-flow patterns and wind loads on facades and the choice of appropriate simulation techniques for the thermal analysis of double skin facades complete the session. In the final Urban Simulation session, a new CFD approach for urban flow and pollution dispersion simulation is presented, a comprehensive micro-simulation of resource flows for sustainable urban planning, multi-scale modelling of the urban climate and the optimisation of urban energy demands using an evolutionary algorithm are discussed.

  6. The Technology Enhanced Conference - A Board Game!

    DEFF Research Database (Denmark)

    Pedersen, Annette

    ITMEDIA at the University of Copenhagen have been working with taking the academic conference online for years. Streaming events, using backchannel chat systems and Twitter, producing introductory pre-event videos, setting up audio debates with keynotes to enrich and prolong the conference...... been great, and maybe the board game can be used for other areas, where one needs to communicate complicated options for technology enhanced events or teaching and support organizers in making good choices....

  7. International Conference on Transformations in Engineering Education

    CERN Document Server

    2015-01-01

    This book comprises the proceedings of the International Conference on Transformations in Engineering Education conducted jointly by BVB College of Engineering & Technology, Hubli, India and Indo US Collaboration for Engineering Education (IUCEE). This event is done in collaboration with International Federation of Engineering Education Societies (IFEES), American Society for Engineering Education (ASEE) and Global Engineering Deans' Council (GEDC). The conference is about showcasing the transformational practices in Engineering Education space.

  8. Third International Conference on Large Meteorite Impacts

    Science.gov (United States)

    2003-01-01

    The Third International Conference on Large Meteorite Impacts (formerly Large Meteorite Impacts and Planetary Evolution) was held August 5-7, 2003, at the Kloesterle, Noerdlingen. The conference addressed a wide range of topics relating to planetary impacts and their effects on planetary crusts, climate, life on Earth, rocks, and rock-forming minerals. This CD-ROM contains the preface, table of contents, program, abstracts and indexes.

  9. International Conference on Extreme Learning Machines 2014

    CERN Document Server

    Mao, Kezhi; Cambria, Erik; Man, Zhihong; Toh, Kar-Ann

    2015-01-01

    This book contains some selected papers from the International Conference on Extreme Learning Machine 2014, which was held in Singapore, December 8-10, 2014. This conference brought together the researchers and practitioners of Extreme Learning Machine (ELM) from a variety of fields to promote research and development of “learning without iterative tuning”.  The book covers theories, algorithms and applications of ELM. It gives the readers a glance of the most recent advances of ELM.  

  10. International Conference on Extreme Learning Machine 2015

    CERN Document Server

    Mao, Kezhi; Wu, Jonathan; Lendasse, Amaury; ELM 2015; Theory, Algorithms and Applications (I); Theory, Algorithms and Applications (II)

    2016-01-01

    This book contains some selected papers from the International Conference on Extreme Learning Machine 2015, which was held in Hangzhou, China, December 15-17, 2015. This conference brought together researchers and engineers to share and exchange R&D experience on both theoretical studies and practical applications of the Extreme Learning Machine (ELM) technique and brain learning. This book covers theories, algorithms ad applications of ELM. It gives readers a glance of the most recent advances of ELM. .

  11. International Conference on Sustainable Energy for Africa

    Directory of Open Access Journals (Sweden)

    Royal Academy for Overseas Sciences

    2017-01-01

    Full Text Available The Royal Academy for Overseas Sciences will organize an International Conference on Sustainable Energy for Africa. This three-day conference will take place from 23 until 25 October 2017 at the Palais des Académies in Brussels. Papers and posters dealing with various themes related to the issue will be presented by academics and representatives of the industrial world. More information will soon be available on the website of the Academy: www.kaowarsom.be.

  12. Autonomous Rendezvous and Docking Conference, volume 1

    Science.gov (United States)

    1990-01-01

    This document consists of the presentation submitted at the Autonomous Rendezvous and Docking (ARD) Conference. It contains three volumes: ARD hardware technology; ARD software technology; and ARD operations. The purpose of this conference is to identify the technologies required for an on orbit demonstration of the ARD, assess the maturity of these technologies, and provide the necessary insight for a quality assessment of the programmatic management, technical, schedule, and cost risks.

  13. Autonomous Rendezvous and Docking Conference, volume 3

    Science.gov (United States)

    1990-01-01

    This document consists of the presentation submitted at the Autonomous Rendezvous and Docking (ARD) Conference. The document contains three volumes: ARD hardware technology; ARD software technology; and ARD operations. The purpose of this conference is to identify the technologies required for an on orbit demonstration of ARD, assess the maturity of these technologies, and provide the necessary insight for a quality assessment of programmatic management, technical, schedule, and cost risks.

  14. 10th World Earthquake Engineering Conference

    Science.gov (United States)

    Ranguelov, Boyko; Housner, George

    The 10th World Conference on Earthquake Engineering (10WCEE) took place from July 19 to 24 in Madrid, Spain. More than 1500 participants from 51 countries attended the conference. All aspects of earthquake engineering were covered and a worldwide update of modern research and practice, as well as future directions in the field, was provided through reports, papers, posters, two keynote lectures, ten state-ofthe-art reports, and eleven special theme sessions.

  15. 7th ICT Innovations 2015 Conference

    CERN Document Server

    Koceski, Saso

    2016-01-01

    This book offers a collection of selected papers presented at the Seventh International Conference on ICT Innovations held in October 2015, in Ohrid, Macedonia, with main topic Emerging Technologies for Better Living. The conference gathered academics, professionals and industrial practitioners that work on developing the emerging technologies, systems, applications in the industrial and business arena especially innovative commercial implementations, novel application of technology, and experience in applying recent ICT research advances to practical solutions.

  16. Computational Intelligence in Information Systems Conference

    CERN Document Server

    Au, Thien-Wan; Omar, Saiful

    2017-01-01

    This book constitutes the Proceedings of the Computational Intelligence in Information Systems conference (CIIS 2016), held in Brunei, November 18–20, 2016. The CIIS conference provides a platform for researchers to exchange the latest ideas and to present new research advances in general areas related to computational intelligence and its applications. The 26 revised full papers presented in this book have been carefully selected from 62 submissions. They cover a wide range of topics and application areas in computational intelligence and informatics.

  17. Third International Joint Conference on Computational Intelligence (IJCCI 2011)

    CERN Document Server

    Dourado, António; Rosa, Agostinho; Filipe, Joaquim; Computational Intelligence

    2013-01-01

    The present book includes a set of selected extended papers from the third International Joint Conference on Computational Intelligence (IJCCI 2011), held in Paris, France, from 24 to 26 October 2011. The conference was composed of three co-located conferences:  The International Conference on Fuzzy Computation (ICFC), the International Conference on Evolutionary Computation (ICEC), and the International Conference on Neural Computation (ICNC). Recent progresses in scientific developments and applications in these three areas are reported in this book. IJCCI received 283 submissions, from 59 countries, in all continents. This book includes the revised and extended versions of a strict selection of the best papers presented at the conference.

  18. Teaching of Psychology Conference: Ideas & Innovations. Proceedings from the Annual Conference (24th, Tarrytown, NY, March 19-20, 2010)

    Science.gov (United States)

    Howell-Carter, Marya, Ed.; Gonder, Jennifer, Ed.

    2010-01-01

    Conference proceedings of the 24th Annual Conference on the Teaching of Psychology: Ideas and Innovations, sponsored by the Psychology Department of the State University of New York at Farmingdale. The conference theme for 2010 was Fostering, Assessing, and Sustaining Student Engagement. The conference featured two keynote addresses from prominent…

  19. 5th International Conference OTEH 2012 - defense technology (Proceedings review)

    OpenAIRE

    Vlado Petar Đurković

    2013-01-01

    This article gives an overview of all papers and events at the Fifth International ScientificConference on Defense Technologies OTEH 2012 held in Belgrade, in the Military Technical Institute (VTI), from 18th to 19th September 2012.The paper review presents the Conference Sections by subjects and guest-lectureres as well as the institutions of all authors who actively participated at the Conference.About the ConferenceThe Fifth International Scientific Conference on Defense Technologies OTEH ...

  20. PREFACE: 13th International Conference on Liquid and Amorphous Metals

    Science.gov (United States)

    Popel, Pjotr; Gelchinskii, Boris; Sidorov, Valeriy; Son, Leonid; Sabirzjanov, Alexandre

    2007-06-01

    The state of the art in the field of liquid and amorphous metals and alloys is regularly updated through two series of complementary international conferences, the LAM (Liquid and Amorphous Metals) and the RQ (Rapidly Quenched Materials). The first series of the conferences started as LM-1 in 1966 at Brookhaven for the basic understanding of liquid metals. The subsequent LM conferences were held in Tokyo (1972) and Bristol (1976). The conference was renewed in Grenoble (1980) as a LAM conference including amorphous metals and continued in Los Angeles (1983), Garmisch-Partenkirchen (1986), Kyoto (1989), Vienna (1992), Chicago (1995), Dortmund (1998), Yokohama (2001) and Metz (2004). The conferences are mainly devoted to liquid and amorphous metals and alloys. However, communications on some non-metallic systems such as semi conductors, quasicrystals etc, were accepted as well. The conference tradition strongly encourages the participation of junior researchers and graduate students. The 13th conference of the LAM series was organized in Ekaterinburg, Russia, by the Institute of Metallurgy of the Ural Branch of the Russian Academy of Sciences (IMet UB RAS) and Ural State Pedagogical University (USPU) and held on 8-13 July 2007 under the chairmanship of Professors Pjotr Popel (USPU) and Boris Gelchinskii (IMet UB RAS). There were 242 active and about 60 guest participants from 20 countries who attended the conference. There were no parallel sessions and all oral reports were separated into three groups: invited talks (40 min), full-scale (25 min) and brief (15 min) oral reports. The program included 10 sessions, ranging from purely theoretical subjects to technological application of molten and amorphous alloys. The following sessions took place: A) Electronic structure and transport, magnetic properties; B) Phase transitions; C) Structure; D) Atomic dynamics and transport; E) Thermodynamics; F) Modelling, simulation; G) Surface and interface; H) Mechanical properties